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Sample records for terapia intermedia estudio

  1. NRE57/12: Plan de Estudios de Graduado o Graduada en Terapia Ocupacional

    OpenAIRE

    Universidad de Granada

    2012-01-01

    Plan de Estudios de Graduado o Graduada en Terapia Ocupacional. Resoluci??n de 22 de febrero de 2012, de la Universidad de Granada, por la que se hace p??blico el Plan de Estudios de Graduado o Graduada en Terapia Ocupacional

  2. Hipnoterapia y terapia breve centrada en soluciones aplicada a síntomas por abuso sexual infantil: Un estudio de caso

    OpenAIRE

    Sánchez, Teresa; Téllez, Arnoldo

    2016-01-01

    En el estudio de caso que se presenta se aplicaron estrategias de hipnosis clínica en un contexto de intervención de terapia breve centrada en soluciones a un paciente de 40 años, víctima de abuso sexual infantil. El paciente presentaba cambios en s

  3. Significado de calidad de vida en pacientes con terapia de hemodiálisis: un estudio fenomenológico

    Directory of Open Access Journals (Sweden)

    Leslie Herbias Herbias

    2016-03-01

    Full Text Available La calidad de vida es uno de los aspectos importantes a abordar en pacientes en hemodiálisis. Objetivo: Comprender el significado de calidad de vida para personas que son sometidas a tratamiento de sustitución renal con hemodiálisis. Metodología: Estudio cualitativo fenomenológico descriptivo. Se realizaron entrevistas en profundidad a 12 personas en hemodiálisis. Las entrevistas fueron grabadas y transcritas textualmente. El análisis se realizó de acuerdo a Streubert, se resguardaron criterios de rigor y se contó con la aprobación del Comité científico de la Facultad de Enfermería de la Universidad Andrés Bello. Resultados: Calidad de vida fue descrita a través de tres categorías comprensivas: Salud, Familia y Actividades de la vida diaria, que involucra ámbitos físicos, psicológicos y sociales. Los participantes significan la calidad de vida cómo Seguir viviendo, ya que producto de la terapia pueden continuar con su vida. Conclusiones: Comprender lo que significa calidad de vida permite ampliar la mirada, fortalecer el vínculo con la familia y considerar lo crucial de esta terapia en la vida de las personas en hemodiálisis. Se sugiere fortalecer la relación enfermera-paciente con el fin de otorgar cuidados seguros y de calidad a los usuarios y sus familias.

  4. Efecto diferenciado de la psicoeducación y de la exposición y prevención de respuesta en la terapia cognitivo-conductual del trastorno obsesivo-compulsivo y estudio de variables moderadoras del tratamiento

    OpenAIRE

    Nogueira, Raquel; Romero, Pablo; Gavino, Aurora

    2015-01-01

    Efecto diferenciado de la psicoeducación y de la exposición y prevención de respuesta en la terapia cognitivo-conductual del trastorno obsesivo-compulsivo y estudio de variables moderadoras del tratamiento Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.

  5. Programa de Terapia de Remotivação em idosos institucionalizados: estudo piloto Programa de Terapia de Remotivación para adultos mayores institucionalizados: estudio piloto Remotivation Therapy Program for institutionalized elders: pilot study

    Directory of Open Access Journals (Sweden)

    Lia Araújo

    2011-12-01

    Full Text Available O desenvolvimento da Terapia de Remotivação na intervenção gerontológica, apesar de recente, tem vindo a demonstrar um impacto positivo, tanto em idosos com demência leve com algum comprometimento funcional e cognitivo, como em idosos autónomos e independentes. As experiências reportadas apontam para uma melhoria da interação entre indivíduos, o que contribui para a sua integração social e aumento da autoestima. Este artigo procura analisar os benefícios de um Programa de Terapia de Remotivação para idosos institucionalizados (ProTR, aos níveis da sintomatologia depressiva, ânimo e participação. Os sujeitos foram 14, divididos em duas condições: grupo experimental (que participou nas sessões do ProTR e grupo de controlo (sem intervenção. Os instrumentos utilizados foram a Escala Geriátrica de Ânimo, a Escala de Depressão Geriátrica e a Escala de Avaliação do Comportamento na Remotivação. Os resultados obtidos sugerem que o ProTR contribuiu para a diminuição da sintomatologia depressiva após a intervenção e para o aumento do ânimo no follow-up. Os benefícios encontrados neste estudo piloto justificam o futuro desenvolvimento e avaliação deste programa, nomeadamente junto de outras populações.El desarrollo de la Terapia de Remotivacióon en la intervención gerontológica, a pesar de ser reciente, ha demostrado tener un impacto positivo tanto en pacientes mayores con demencia leve con alguno deterioro funcional y cognitivo, como en mayores autónomos e independientes. Los experimentos reportados señalan una mejora de la interacción entre individuos, lo que contribuye a su integración y aumento de la autoestima. Este estudio procura analizar los beneficios de un Programa de Terapia de Remotivación para adultos mayores institucionalizados (ProTR en lo que concierne los niveles de sintomatología depresiva, ánimo y participación. Hubo 14 articipantes, divididos en dos grupos: el experimental (que

  6. Regression of extramedullary hematopoiesis with hydroxyurea therapy in ß-thalassemia intermedia Regressão da hematopoese extramedular na talassemia intermédia após terapia com hidroxiuréia

    Directory of Open Access Journals (Sweden)

    Perla Vicari

    2006-03-01

    Full Text Available Excessive ineffective erythropoiesis in thalassemia intermedia may cause extramedullary hematopoiesis (EMH, resulting in spleen and liver enlargement or masses in several tissues, mainly paravertebrally. Other less frequent locations of diffuse compensatory EMH are kidneys, adrenal glands, breasts, spinal cord, pleura, pericardium, duramater, adipose tissue and skin, although intrathoracic extramedullary hematopoiesis is a rare condition. Management strategies have included radiation and transfusion therapy. Hydroxyurea with transfusion therapy has been associated with clinical regression of EMH in thalassemia. We report an uncommon case of intrathoracic EMH in a patient with beta-thalassemia intermedia, that showed significant recovery with HU therapy.A excessiva eritropoese ineficaz na talassemia pode causar hemato-poese extramedular (HEM, resultando em hepatomegalia, esplenomegalia e massas de tecido hematopoético em diversos tecidos. Localizações de HEM compensatória menos freqüentes são rins, glândulas adrenais, canal medular, pleura, pericárdio, duramáter, tecido adiposo e pele. Entretanto, HEM intratorácica é condição rara. Estratégias terapêuticas incluem radiação e transfusões sanguíneas. O uso de hidroxiuréia concomitante a terapêutica transfusional foi associado à regressão clínica da HEM na talassemia. Nós descrevemos um caso de HEM intratorácica em paciente portadora de talassemia intermédia, com significante regressão do quadro após terapêutica isolada com hidroxiuréia.

  7. Aplicación de la terapia de aceptación y compromiso (ACT a la conducta inapetente: un estudio de caso

    Directory of Open Access Journals (Sweden)

    Kevin Egúsquiza Vásquez

    2015-12-01

    Full Text Available RESUMEN: En este artículo se presenta un estudio de caso acerca de la conducta inapetente abordado desde la Terapia de Aceptación y Compromiso. En primer lugar, se concibió a la evitación experiencial como una dimensión transdiagnóstica común a diversas problemáticas humanas. Seguidamente, se revisó la historia del participante y se prosiguió con la evaluación del caso mediante el Cuestionario Parental de Aceptación y Acción (PAAQ y un registro de duración de conducta, en base a todo ello se realizó un análisis funcional del caso. Posteriormente, se aplicó la Terapia de Aceptación y Compromiso (ACT con la finalidad de romper el repertorio de evitación y encaminar la vida de los principales involucrados hacia las acciones que se consideren valiosas; tras ello, se realizaron tres seguimientos. Finalmente, se analizaron y discutieron los resultados obtenidos a partir de los exitosos beneficios topográficos y funcionales reflejados de forma contingente a la aplicación de la ACT en el campo de los problemas de conducta alimentaria. ABSTRACT: In this article a case study on the loss of appetite approached from the Acceptance and Commitment Therapy is presented. First, experiential avoidance was conceptualized as a transdiagnostic dimension common to various human problems. Then, the story of the participant was reviewed and the assessment of the case was done by the Parental Acceptance and Action Questionnaire (PAAQ and a record length of conduct, based on all functional analysis of the case was conducted. Subsequently, Acceptance and Commitment Therapy (ACT was applied in order to break the avoidant repertoire and route the path of life towards the valued actions, after that three follow-ups were conducted. Finally, the results obtained from topographic and functional successful benefits were analyzed and discussed, contingent on the application of ACT in the field of eating behavior problems.

  8. INCORPORACIÓN Y CUMPLIMIENTO DE LA TERAPIA DE REEMPLAZO HORMONAL DEL CLIMATERIO FEMENINO ANTES Y DESPUÉS DEL ESTUDIO DEL WHI: REVISIÓN DE LA EVIDENCIA

    OpenAIRE

    Omar Arena F.,Jorge

    2005-01-01

    Objetivo: analizar los factores que afectan y/o determinan el inicio y cumplimiento de la terapia hormonal en el climaterio, y el efecto de como el estudio WHI y los trabajos vinculados a éste han impactado sobre esta terapéutica. Metodología: Se realizó una búsqueda de la información que hace referencia al tema en cuestión en las bases de datos MEDLINE y LILACS, seleccionando los trabajos para el análisis que presentaran mayor rigor científico, que fueran publicados en revistas arbitradas qu...

  9. Thalassemia intermedia: An overview

    African Journals Online (AJOL)

    Rabah M. Shawky

    2012-04-25

    Apr 25, 2012 ... Mutations that dis- rupt or delete either enhancer or LCR sequences interfere with. 246. R.M. Shawky, T.M. Kamal ... [23,28]. Hemolysis per se has been linked to the develop- ment of a hypercoagulable state and ...... Thalassemia intermedia: genetic and biochemical considerations. Ann N Y Acad Sci 1964 ...

  10. Terapia periodontal no quirúrgica

    OpenAIRE

    Botero Zuluaga, Leticia; Botero Botero, Alejandro; Bedoya Trujillo, Juan Sebastián; Guzmán Zuluaga, Isabel Cristina

    2012-01-01

    Introducción: en el tratamiento de las enfermedades periodontales contamos con la terapia periodontal no quirúrgica, la cual ha sido avalada científicamente mostrando su efectividad. El principal objetivo de este artículo es demostrar la efectividad de la terapia periodontal no quirúrgica evidenciada en múltiples estudios con relación a las indicaciones, aspectos microbiológicos, efectos en los tejidos y la importancia de la terapia de mantenimiento una vez finalizado el tratamiento. Métodos:...

  11. Estudio de células troncales mesenquimales para su empleo como vectores en terapias antitumorales: Visualización mediante técnicas de imagen molecular

    OpenAIRE

    Belmar López, Carolina; Martín Duque, María Pilar

    2013-01-01

    El cáncer es la enfermedad con mayor tasa de mortalidad en nuestros días, causando una muerte de cada cuatro casos, en USA en el año 2010 (Jemal et al, 2010). A pesar de los avances, muchos tipos de tumores siguen sin posibilidad de un tratamiento eficaz, por lo que la investigación en nuevas terapias es necesario. Las células malignas o tumorales no se encuentran solas, si no que conviven en un complejo microambiente denominado como estroma tumoral (Albini et al, 2007) y que pueden ser cara...

  12. THALASSAEMIA INTERMEDIA : AN UPDATE

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    Ali Taher

    2009-08-01

    Full Text Available Our understanding of the molecular and pathophysiological mechanisms underlying the disease process in patients with thalassaemia intermedia (TI has substantially increased over the past decade. TI encompasses a wide clinical spectrum of beta-thalassaemia phenotypes. Some TI patients are asymptomatic until adult life, whereas others are symptomatic from as young as 2 years of age. A number of clinical complications commonly associated with TI are rarely seen in thalassaemia major, including extramedullary hematopoiesis, leg ulcers, gallstones, thrombosis and pulmonary hypertension. There are a number of options currently available for managing patients with TI, including transfusion therapy, iron chelation therapy, modulation of foetal haemoglobin production and haematopoietic stem cell transplantation. However, at present, there are no clear guidelines for an orchestrated optimal treatment plan.

  13. THALASSAEMIA INTERMEDIA : AN UPDATE

    Directory of Open Access Journals (Sweden)

    Maria Domenica Cappellini

    2009-06-01

    Full Text Available

    Our understanding of the molecular and pathophysiological mechanisms underlying the disease process in patients with thalassaemia intermedia (TI has substantially increased over the past decade. TI encompasses a wide clinical spectrum of beta-thalassaemia phenotypes. Some TI patients are asymptomatic until adult life, whereas others are symptomatic from as young as 2 years of age. A number of clinical complications commonly associated with TI are rarely seen in thalassaemia major, including extramedullary hematopoiesis, leg ulcers, gallstones, thrombosis and pulmonary hypertension. There are a number of options currently available for managing patients with TI, including transfusion therapy, iron chelation therapy, modulation of foetal haemoglobin production and haematopoietic stem cell transplantation. However, at present, there are no clear guidelines for an orchestrated optimal treatment plan.

  14. CITYMARKETING EN CIUDADES INTERMEDIAS: CASO PALMIRA

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    Miguel Ezequiel Badillo Mendoza

    2010-01-01

    Full Text Available En este artículo se presentan los resultados de la investigación Citymarketing en ciudades intermedias: caso Palmira, específicamente en su etapa de diseño. Este proyecto surgió de la necesidad de generar acciones académicas y metodológicas para desarrollar un mecanismo que permitiera el diseño de un instrumento de gestión y promoción de la ciudad. La población de estudio se integró con habitantes de la ciudad, visitantes y dirigentes de diversos sectores del departamento del Valle del Cauca. La metodología empleada se basó en un estudio documental y el uso de instrumentos cualitativos, como cartografías urbanas, estudio de imaginarios y mediciones cuantitativas de comunicación publicitaria. Para su realización se estructuró en dos fases: La primera donde se estableció un eje conceptual para la implementación de un Plan de citymarketing en Palmira y una fase 2: donde se desarrolló la sensibilización, la caracterización de la ciudad, los lineamientos estratégicos, los parámetros para la construcción de la marca y el diseño del Plan de Comunicaciones.

  15. Niveles de bioelectricidad en amalgamas con y sin base intermedia

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    John Alexis Dominguez

    Full Text Available Los nuevos materiales estéticos no han ocasionado el abandono de las obturaciones de amalgama, es por eso que es importante proponer elementos que le den longevidad a las restauraciones. El propósito de este estudio fue determinar la relación entre los niveles de bioelectricidad y la presencia de base intermedia en obturaciones de amalgama. El presente estudio es un diseño de tipo descriptivo transversal, la población de estudio se conformó por un total de 30 obturaciones de pacientes que fueron atendidos en la Clínica Odontológica de la Universidad Cooperativa de Colombia de la ciudad de Pasto en el segundo semestre académico del 2007, los niveles de bioelectricidad se midieron en microamperios con un tester Techma TM-086 en sensibilidad de 20 µA. El mayor promedio de bioelectricidad lo generaron las restauraciones de amalgama que no presentaron base intermedia con un promedio de 0,02867 µA (Mann-Whitney - p=0,031 con respecto a las que tenían base intermedia con un promedio de -0.07133 µA , y la marca de amalgama que mas bioelectricidad generó fue la New Stetic con un promedio de 0,01535 µA (Kruskal wallis - p=0,008 con respecto a SDI con un promedio de -0.1633 µA y Kerr con un promedio de -0.098 µA. La intensidad del pasaje de corriente disminuye en las obturaciones de amalgamas cuando presentan base intermedia.

  16. Estudio de la pérdida dental en pacientes diagnosticados de periodontitis, tratados y controlados con terapia periodontal de soporte

    OpenAIRE

    Estefanía Fresco, Ruth

    2016-01-01

    173 p. Los objetivos son analizar la pérdida dental en los pacientes diagnosticados de periodontitis crónica o agresiva, tratados y mantenidos durante un mínimo de 5 años, así como reconocer cuáles son los factores de riesgo involucrados en la pérdida dental en estos pacientes. Para ello se diseñó un estudio retrospectivo y observacional, con un tamaño muestral de 175 pacientes. Los datos se recogieron revisando la historia clínica y los registros radiográficos, mediante una entrevista con...

  17. Forma intermedia de la enfermedad de Charcot‐Marie‐Tooth

    OpenAIRE

    Martínez Tamés, Míriam

    2017-01-01

    En este estudio, hemos re‐evaluado dos familias de Charcot‐Marie‐Tooth intermedia con herencia autosómica dominante (DI‐CMT). La primera familia está asociada a la mutación E396K del gen NEFL, y la segunda a la mutación N98S de este gen. La primera familia incluye cuatro pacientes en dos generaciones con edades comprendidas entre los 35 y los 59 años, cuyo fenotipo se caracteriza por neuropatía sensitivo‐motora dependiente de la longitud, que en dos casos afectó a la musculatura pelviana, y s...

  18. Efecto del uso de la terapia de reemplazo hormonal en la calidad de vida de las mujeres postmenopáusicas: Estudio descriptivo tipo análisis de tendencia. Consulta externa de ginecología, Hospital San Juan de Dios

    OpenAIRE

    Chang Lin, Sing-Hung; Montvelisky Karolicki, Herman

    2013-01-01

    En las últimas décadas, se ha dado un creciente interés en la comunidad científica mundial en investigar los cambios fisiológicos que acompañan al climaterio y los medicamentos que conforman la terapia de reemplazo hormonal (TRH), principalmente los estrógenos. Se han hecho inversiones cuantiosas para llevar a cabo los múltiples estudios sobre los efectos clínicos, beneficios y riesgos de estas hormonas, cuyos resultados muchas veces son contradictorios y no concluyentes (Notelovitz y Kanmaz...

  19. Severity of trauma victims admitted in intensive care units: comparative study among different indexes Gravedad de las víctimas de trauma, admitidas en unidades de terapia intensiva: estudio comparativo entre diferentes índices Gravidade das vítimas de trauma, admitidas em unidades de terapia intensiva: estudo comparativo entre diferentes índices

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    Lilia de Souza Nogueira

    2009-12-01

    Full Text Available This study compared the performance of the Injury Severity Score (ISS with the New Injury Severity Score (NISS and also the Simplified Acute Physiology Score II (SAPS II with the Logistic Organ Dysfunction System (LODS in trauma victims, in order to predict mortality and length of stay in Intensive Care Units (ICU, besides identifying which indexes have been the most effective to estimate these results. A retrospective analysis was done in the records of 185 victims admitted in ICU between June and December 2006. None of the four indexes properly discriminated the patients according to length of stay at the ICU. The ISS and the NISS did not show a good discriminating capacity in case of death, but the SAPS II and the LODS presented good performance to estimate mortality at the ICU. Results pointed towards the use of SAPS II and LODS when trauma victims are admitted in an ICU.Este estudio tuvo por objetivo comparar en víctimas de trauma el desempeño del Injury Severity Score (IS, con el New Injury Severity Score (NIS y, también, del Simplified Acute Physiology Score II (SAPS II, con el Logistic Organ Dysfunction System (LODS para predecir la mortalidad y el tiempo de permanencia en unidades de terapia intensiva (UTI, y también para identificar cuales índices fueron los más efectivos para estimar esos resultados. Fue realizado un análisis retrospectivo de las fichas de 185 víctimas, admitidas en una UTI, entre junio y diciembre de 2006. Los cuatro índices no discriminaron adecuadamente a los pacientes según el tiempo de permanencia en la UTI. El IS y el NIS no mostraron una buena capacidad discriminatoria para la ocurrencia de muerte, diferente del SAPS II y del LODS que presentaron un mejor desempeño para estimar la mortalidad en UTI. Los resultados apuntaron para el uso del SAPS II y del LODS cuando víctimas de trauma son internadas en una UTI.Este estudo objetivou comparar em vítimas de trauma o desempenho do Injury Severity Score

  20. La investigación en terapia familiar

    OpenAIRE

    Díaz Oropeza, Ismael Fernando

    2016-01-01

    El propósito de este trabajo es presentar un panorama sobre el desarrollo de la investigación empírica en la terapia familiar. Por lo cial, se abordan las tradiciones de investigación en psicoterapia, a saber los estudios sobre los resultados, los procesos y el progreso. Estas tradiciones han ido generando nuevas tendencias de indagación, como son los estudios sobre los factores comunes y específicos, la efectividad y la eficacia de la terapia y el intento de validar empíricamente los tratami...

  1. Terapia de choque

    DEFF Research Database (Denmark)

    Agustin, Oscar Garcia

    2012-01-01

    No hay alternativa, nos dicen. Ya no hay líneas rojas. Sólo un poco más de terapia de choque para aumentar el desorden bipolar entre los que tienen y los que no.......No hay alternativa, nos dicen. Ya no hay líneas rojas. Sólo un poco más de terapia de choque para aumentar el desorden bipolar entre los que tienen y los que no....

  2. Estudo comparativo do consumo e gasto com medicamentos em Unidades Pediátricas de Terapia Intensiva e Semi-intensiva Estudio comparativo del consumo y gasto con medicamentos en Unidades Pediátricas de Terapia Intensiva y Semi-intensiva Comparative study of drug utilization and drug expenses in Pediatric Intensive and Semi-intensive Care Units

    Directory of Open Access Journals (Sweden)

    Larissa Lenotti Zuliani

    2012-01-01

    Full Text Available OBJETIVO: Descrever e comparar o consumo e gastos com medicamentos em Unidades de Terapia Intensiva (UTI e Semi-intensiva Pediátricas. MÉTODOS: Estudo descritivo, exploratório, retrospectivo, com abordagem quantitativa por meio de estatística descritiva simples. A coleta de dados ocorreu no período de junho de 2007 a maio de 2008 nas UTI e Semi-intensiva Pediátricas de um hospital de ensino do interior do Estado de São Paulo, utilizando-se a classificação ABC. RESULTADOS: O gasto médio/leito da Unidade Cardiológica foi de R$ 1.400,00±0,26 leito/mês e da Neonatal de R$ 1.530,00±0,27 leito/mês, sendo menor na UTI e Semi-intensiva Pediátrica (R$ 260,00±0,13 leito/mês. Houve variação significativa do gasto mensal com medicamentos independentemente da taxa de ocupação. Na Classe A, os dez medicamentos de maior custo representaram 57,1, 54,3, e 46,3% do orçamento das UTI e Semi-intensivas Cardiológica, Neonatal e Pediátrica, respectivamente. Na Neonatal, os dez medicamentos mais consumidos corresponderam à Classe C, com 6,6% do orçamento, enquanto que nas outras unidades se enquadram oito, responsáveis por 7,8% do orçamento da Cardiológica e 7,7% da Pediátrica. CONCLUSÕES: A classificação ABC permitiu conhecer o consumo e os gastos com medicamentos; esse método favorece a gestão desses recursos nas unidades avaliadas.OBJETIVO: Describir y comparar el consumo y los gastos con medicamentos en Unidades de Terapia Intensiva (UTI y Semi-intensiva Pediátricas. MÉTODOS: Estudio descriptivo, exploratorio, retrospectivo, con abordaje cuantitativo mediante estadística descriptiva simple. La recolección de datos ocurrió en el periodo de junio de 2007 a mayo de 2008 en las UTI y Semi-intensiva Pediátricas de un hospital de enseñanza en el interior del Estado de São Paulo, utilizándose la clasificación ABC. RESULTADOS: El gasto medio/lecho de las Unidades Cardiológicas fue de R$ 1.400,00±0,26 lecho/mes y Neonatal de

  3. Atenção paliativa oncológica em Unidade de Terapia Intensiva: um estudo da produção científica da enfermagem Atención paliativa oncológica en Unidad de Terapia Intensiva: un estudio de la producción científica de la enfermería Cancer palliative care in an Intensive Care Unit: a scientific production study of Nursing

    Directory of Open Access Journals (Sweden)

    Ana Carolina Abeid Mendonça

    2012-12-01

    Full Text Available O estudo teve como objetivo analisar a produção científica da enfermagem na atenção paliativa oncológica em unidades de terapia intensiva. Pesquisa exploratória, descritiva, retrospectiva e bibliográfica de 23 artigos produzidos de 2000 a 2010. O referencial teórico-metodológico foi a Metodologia de Categorização Epistemológica para a Pesquisa na Enfermagem com ênfase na constituição do tema ou problema da pesquisa. Os resultados indicam que o conhecimento produzido concentra-se no âmbito internacional, setorizado predominantemente na unidade de terapia intensiva adulto. A análise dos temas focalizados indica a apreensão do fenômeno na esfera subjetiva, na ótica dos enfermeiros como sujeitos/consciência do conhecimento para apreensão de situações de enfermagem relacionadas às estratégias e obstáculos à implantação da atenção paliativa oncológica nesse cenário, além das contribuições da atenção paliativa oncológica para clientes e familiares. As repercussões demonstradas nos aspectos epistemológicos destacados possibilitam afirmar que a assistência de enfermagem a pessoas com câncer avançado sem possibilidades de cura na Unidade de Terapia Intensiva é permeada por desafios que requerem investigações para subsidiar critérios e estratégias para atuação da equipe de enfermagem para essa clientela.El estudio tuvo como objetivo analizar la producción científica de enfermería en la atención paliativa oncológica en Unidades de Terapia Intensiva (UTI. Investigación exploratoria, descriptiva, retrospectiva y bibliográfica de 23 artículos producidos entre 2000 y 2010. El referencial teórico-metodológico fue la Metodología de Categorización Epistemológica para Pesquisa en Enfermería con énfasis en la constitución del tema o problema de la investigación. Los resultados indican que el conocimiento producido se concentra en el ámbito internacional, sectorizada predominantemente en la unidad

  4. La terapia manual osteopática

    OpenAIRE

    Escarmis i Costa, Francesc; Martínez Gil, José Luis

    2001-01-01

    Este trabajo desarrolla brevemente la historia de la Osteopatía, su introducción en Europa y cómo desde Europa ha ido viniendo a España de la mano de los Fisioterapeutas. Resume así mismo la Filosofía de la Osteopatía y sus principios. Y, por último, nos revela la situación actual de la Osteopatía en España y la necesidad que creemos que existe para su mayor desarrollo, estudio e integración dentro de la Fisioterapia. Terapia y Rehabilitación

  5. Terapia cognitivo conductual integrativa

    OpenAIRE

    Fernández-Álvarez, Héctor; Fernández-Álvarez, Javier

    2017-01-01

    En los últimos años ha surgido un interés creciente por integrar los diversos enfoques que cohabitan en la terapia cognitivo conductual (TCC). Sin embargo, la integración en la TCC puede ser identifi cada desde sus comienzos. Este artículo presenta

  6. Psoriasis y nuevas terapias

    Directory of Open Access Journals (Sweden)

    Edgardo N. Chouela R., Dr.

    2011-11-01

    Esta reseña de las nuevas terapias disponibles de la psoriasis y de las que están en camino de ser aprobadas por las autoridades sanitarias, permitirá al lector tener una idea del estado actual del tratamiento de esta enfermedad.

  7. Degradation of human hemoglobin by Prevotella intermedia.

    Science.gov (United States)

    Guan, Su-Min; Nagata, Hideki; Shizukuishi, Satoshi; Wu, Jun-Zheng

    2006-01-01

    In this study, the ability of Prevotella intermedia, an obligate anaerobic rod, to degrade human hemoglobin was determined by SDS-PAGE and the degradation was quantified by scanning densitometry. Both bacterial cells and culture supernatants degraded hemoglobin. The hemoglobin degradation by P. intermedia was time-dependent, heat sensitive, pH related and was not influenced by iron restriction. Inhibition studies demonstrated that a cysteine protease might be involved in hemoglobin degradation and this protease might require metal ions for its activity and it might be thiol-requiring and trypsin-inducible. The results indicate that P. intermedia is capable to release heme from hemoglobin, hence provide a source of iron for its proliferation.

  8. Complete Genome Sequence of Prevotella intermedia Strain 17-2

    OpenAIRE

    Nambu, Takayuki; Yamane, Kazuyoshi; Maruyama, Hugo; Mashimo, Chiho; Yamanaka, Takeshi

    2015-01-01

    Prevotella intermedia, a Gram-negative black-pigmented anaerobic rod, is frequently isolated from not only periodontal pockets but also purulent infections. We report here the complete genome sequence of P.?intermedia strain 17-2, which is a non-exopolysaccharide-producing variant obtained from exopolysaccharide (EPS)-producing P.?intermedia strain 17 stock culture.

  9. Transfusion regimens in thalassemia intermedia

    Directory of Open Access Journals (Sweden)

    Z. Karakas

    2011-12-01

    Full Text Available Thalassemia intermedia (TI is a heterogeneous disease, in terms of both clinical manifestations and underlying molecular defects. Some TI patients are asymptomatic until adult life, whereas others are symptomatic from early childhood. In contrast with patients with Thalassemia major (TM, the severity of anemia is less and the patients do not require transfusions during at least the first few years of life. Many patients with TI, especially older ones, have been exposed to the multiple long-term effects of chronic anemia and tissue hypoxia and their compensatory reactions, including enhanced erythropoiesis and increased iron absorption. Bone marrow expansion and extramedullary hematopoiesis lead to bone deformities and liver and spleen enlargement. Therapeutic strategies in TI are not clear and different criteria are used to decide the initiation of transfusion and chelation therapy, modulation of fetal hemoglobin production, and hematopoietic stem cell transplantation on an individual basis. The clinical picture of well-treated TM patients with regular transfusionchelation therapy is better from TI patients who have not received adequate transfusion therapy. There is a significant role of early blood transfusion to prevent and treat complications commonly associated with TI, such as extramedullary erythropoiesis and bone deformities, autoimmune hemolytic anemia, leg ulcers, gallstones, pseudoxantoma elasticum, hyperuricosuria, gout and pulmonary hypertension, which are rarely seen in thalassemia major. Nowadays, indications of transfusion in patients with TI are chronic anemia (Hb < 7 g/dL, bone deformities, growth failure, extramedullary erythropoiesis, heart failure, pregnancy and preparation for surgical procedures. Conclusion: Adequate (regular or tailored transfusion therapy is an important treatment modality for increasing the quality of life in patients with thalassemia intermedia during childhood. 就临床表象和潜在的分子缺

  10. Historia e institucionalización de la terapia neural en Colombia

    OpenAIRE

    Sarmiento Rodríguez, Luz Dary

    2014-01-01

    La historia de la Terapia Neural que el mundo médico conoce data de tan sólo nueve décadas atrás, con la apertura generada por los estudios de los hermanos Huneke. Investigaciones recientes permiten concluir que las bases científicas de la Terapia Neural parten de la corriente rusa, desde la primera mitad del siglo XIX, a través de la escuela del Nervismo1 . La Terapia Neural en los países occidentales se encuentra en un proceso de definirse como un sistema médico, lo que exige...

  11. Study on the hemolysin from Prevotella intermedia and Prevotella nigrescens

    OpenAIRE

    鎌口, 有秀; 中村, 麗子; 岡本, 公彰; 馬場, 久衛; カマグチ, アリヒデ; ナカムラ, レイコ; オカモト, マサアキ; ババ, ヒサエ; Arihide, KAMAGUCHI; Reiko, NAKAMURA; Masaaki, OKAMOTO; Hisae, BABA

    2004-01-01

    Hemolysin genes of Prevotella intermedia that have been reported were not detected by PCR in many strains of P. intermedia and Prevotella nigrescens. In both organisms, hemolytic activity was observed in many strains which had not retained the hemolysin genes. Hemolysin was partially purified from the outer membranes of P. intermedia and P. nigrescens. This hemolysin was activated with SH reagents and inhibited with SH blockers. After heating at 100℃ for 10 minutes, the hemolytic activity was...

  12. Complete Genome Sequence of Prevotella intermedia Strain 17-2.

    Science.gov (United States)

    Nambu, Takayuki; Yamane, Kazuyoshi; Maruyama, Hugo; Mashimo, Chiho; Yamanaka, Takeshi

    2015-08-20

    Prevotella intermedia, a Gram-negative black-pigmented anaerobic rod, is frequently isolated from not only periodontal pockets but also purulent infections. We report here the complete genome sequence of P. intermedia strain 17-2, which is a non-exopolysaccharide-producing variant obtained from exopolysaccharide (EPS)-producing P. intermedia strain 17 stock culture. Copyright © 2015 Nambu et al.

  13. Terapia Génica

    Directory of Open Access Journals (Sweden)

    Ignacio Zarante

    2001-08-01

    Full Text Available

    Definición
    La terapia génica es el proceso por el cual se inserta material genético en una célula, con el fin de hacer que ésta produzca una proteína normal. Las utilidades van desde curar enfermedades unigénicas hasta modificar el equilibrio del sistema inmune, permitiendo la modulación de la respuesta contra cualquier antígeno. En esencia es cambiar la secuencia del genotipo de un organismo para que tenga implicaciones fenotípicas.

    Historia
    Desde el descubrimiento de las enzimas de restricción en el año de 1970 por Arber y Hamilton se sentaron las bases para transferir genes entre diferentes células u organismos, inclusive pertenecientes a diferentes especies. En 1978 se realizó la primera hormona recombinante insertando el gen de la insulina en una bacteria E. coli. De allí en adelante se afianzaron los conocimientos necesarios para transferir genes a células humanas con el fin de alterar el fenotipo patológico y generar una nueva forma terapéutica. La primera transferencia se realizó en el año de 1989 en un paciente con una inmunodeficiencia. Aunque no se encontraron efectos clínicos se explicitó que tampoco había efectos deletéreos como muchos apocalípticamente habían pronosticado. En 1990 se trató con terapia génica un paciente que padecía de la deficiencia de la enzima adenosin-deaminasa presentando infecciones bacterianas a repetición.

    Aunque la mejoría fue temporal, con este ensayo se comprobó que la terapia génica tenía posibilidades terapéuticas reales....

  14. Psoriasis y nuevas terapias

    OpenAIRE

    Chouela R., Edgardo N.

    2011-01-01

    La psoriasis es una enfermedad compleja, sistémica y crónica, que compromete la calidad de vida de los pacientes desde muy temprana edad y que requiere del compromiso del médico tratante para su manejo terapéutico. El mejor conocimiento de la fisiopatología de la enfermedad ha permitido el desarrollo de nuevas terapéuticas, algunas ya disponibles y otras en vías de serlo en los próximos años. Esta reseña de las nuevas terapias disponibles de la psoriasis y de las que están en camino de ...

  15. Proyecto y construcción de la losa intermedia del tramo de Santa Coloma de la Línea 9 del ferrocarril metropolitano de Barcelona

    OpenAIRE

    Buil Adrados, Alberto; Arranz Marco, Tomás; Ramos Schneider, Gonzalo; Ainchil Lavín, Javier Pablo

    2008-01-01

    La configuración del Túnel de la Línea 9 del Ferrocarril Metropolitano de Barcelona con una sección mayoritariamente en dos niveles obliga a un estudio minucioso de la solución de la losa intermedia de separación de ambos sentidos de circulación. Frente a la solución convencional de pórtico in situ ejecutado con carro integral en hormigón armado, se ha desarrollado una solución alternativa con la que obtiene un elevado grado de industrialización. La losa intermedia se ha ejecutado mediante un...

  16. Regeneración de defectos infraóseos no contenidos mediante terapia combinada de Emdogain® y BoneCeramic®. Estudio clínico controlado aleatorizado a doble ciego

    OpenAIRE

    Losada Martínez, Meritxell

    2016-01-01

    El presente estudio tiene como objetivo evaluar la regeneración periodontal de defectos infraóseos que, por sus características anatómicas, son no contenidos. Este tipo de defectos, anchos y con un número de paredes remanentes menor, tienen un potencial de regeneración inferior que aquéllos considerados más contenidos. Por un lado, las propiedades estructurales del defecto para albergar el coágulo son menores y, por otro lado, presentan una menor vascularización debido a la reducción del núm...

  17. Isolation and characterization of Yersinia intermedia strains from pig ...

    African Journals Online (AJOL)

    A total of 150 samples of pig tonsils were collected from slaughterhouse in Abidjan; the pigs were from pig farms located in different areas of Côte d'Ivoire. Samples were examined for the presence of Yersinia intermedia. Optimal recovery of Y. intermedia was achieved using two step enrichment procedures based on ...

  18. CATETERES EM TERAPIA INTENSIVA

    Directory of Open Access Journals (Sweden)

    Carolina de Deus Lisboa

    2014-01-01

    Full Text Available Pesquisa com os objetivos de identificar falhas na administração de medicamentos por sondas e caracterizar a interrupção ou não da nutrição no caso de medicamentos que exigem jejum relativo. Estudo epidemiológico, transversal, observacional, numa terapia intensiva, com amostra de 350 doses administradas por 56 técnicos de enfermagem. Resultados mostraram que não houve pausa entre a administração do medicamento e a infusão de dieta enteral em 116 (33,14% doses de medicamentos que necessitavam de jejum relativo, entre os quais captopril, varfarina sódica, levotiroxina sódica, digoxina e fenitoína sódica. A irrigação das sondas não ocorreu na maioria dos casos (94,28%. Conclui-se que é possível que os medicamentos citados tenham tido sua biodisponibilidade sérica reduzida, comprometendo sua eficácia terapêutica e que a falta da prática de irrigar sondas com água estéril, antes de administrar medicamentos, configura-se como a ausência de um cuidado específico fundamental para evitar a obstrução das mesmas.

  19. Does the frequency of Prevotella intermedia increase during pregnancy?

    Science.gov (United States)

    Gürsoy, M; Haraldsson, G; Hyvönen, M; Sorsa, T; Pajukanta, R; Könönen, E

    2009-08-01

    The former Bacteroides intermedius, currently including Prevotella intermedia and Prevotella nigrescens, has been associated with hormone-induced pregnancy gingivitis. The aim of the present longitudinal study was to determine whether only P. intermedia or P. nigrescens, or both species, are involved in the demonstrated microbial shift during pregnancy. Subgingival plaque and saliva samples, collected from 30 healthy pregnant women and 24 healthy non-pregnant women as their controls, were examined for the presence of pigmented gram-negative anaerobes. Altogether 2628 isolates were preliminarily identified as P. intermedia sensu lato, based on phenotypic testing. Their further identification was performed by using a 16S ribosomal DNA-based polymerase chain reaction (PCR). A mean of 8.3 P. intermedia sensu lato isolates from each subject/sampling was examined. During the second trimester, the mean number of P. intermedia sensu lato in plaque increased along with increasing signs of pregnancy gingivitis, and then both decreased. After delivery, gingival inflammation still decreased while the number of P. intermedia sensu lato transiently increased both in plaque and saliva. In the present study, the vast majority of isolates (95.3%) proved to be P. nigrescens and 2.5% were P. intermedia. The remaining 2.2% of the isolates could not be identified with PCR as P. intermedia or P. nigrescens. The corresponding percentages in the control population were 94.2%, 5.5%, and 0.3%. In the oral cavity of relatively young women without periodontitis, P. nigrescens, unlike P. intermedia, is a frequent finding. Conceivably, pregnant women harbor increasing numbers of P. nigrescens associated with pregnancy gingivitis.

  20. Ruptured Massa Intermedia Secondary to Hydrocephalus.

    Science.gov (United States)

    El Damaty, Ahmed; Langner, Soenke; Schroeder, Henry W S

    2017-01-01

    We report a case of ruptured massa intermedia (MI) as a sequela of hydrocephalus. A single case report is presented describing the sequelae of tumor bed hematoma after a posterior fossa hemangioblastoma resection in which the patient bled 3 days after surgery, resulting in secondary hydrocephalus and subsequently dilatation of the third ventricle, which resulted in rupture of the MI. The patient was managed on emergency basis with an external ventricular drain then endoscopically with a third ventriculostomy and clot extraction. Absent MI is not uncommon in hydrocephalic patients, and it is assumed to be the result of rupture from acute dilatation of the third ventricle. Our case report proves this assumption and documents the presence and absence of the MI before and after developing hydrocephalus. Copyright © 2016 Elsevier Inc. All rights reserved.

  1. Terapia de linguagem na afasia

    Directory of Open Access Journals (Sweden)

    Mauro Spinelli

    1977-12-01

    Full Text Available A terapia da linguagem na afasia foi estudada em 15 pacientes, 8 do sexo masculino e 7 do sexo feminino; a idade variou de menos de 10 anos a mais de 50 anos; em 11 casos a afasia era sequela de acidente vascular cerebral e, em 4, de traumatismo crânio-encefálico. Quanto ao tipo de afasia, 5 pacientes apresentavam déficits predominantemente de expressão e 10 apresentavam déficits receptivos e expressivos em graus equiparáveis. Em 6 casos a terapia foi iniciada dentro dos 3 primeiros meses de instalação do quadro; nos restantes foi iniciada mais tardiamente. Dos 15 pacientes, 11 apresentaram evolução satisfatória, a qual não depndeu do grau de afasia, do tipo de afasia, da idade do paciente, do tipo de patologia e do intervalo entre a instalação do quadro afásico e o início da terapia. A terapia da linguagem no paciente afásico é efetiva, o prognóstico não pode ser feito a priori. Somente a evolução do caso com sua retestagem criteriosa poderá dar dados realmente úteis para se falar em evolução.

  2. Uso de kits desechables para optimizar tiempos, movimientos y apego a protocolos de la terapia intravenosa

    OpenAIRE

    José Juan Escamilla Zamudio; Gretta Itzel Ramírez Chávez

    2017-01-01

    Introducción: En México un 95% de los pacientes hospitalizados requerirán un acceso vascular y terapia intravenosa; sin embargo, el uso de intervenciones estandarizadas favorece a la prevención y reducción de infecciones asociadas a catéter vascular. El objetivo es demostrar el beneficio al implementar el uso de kits desechables en procedimientos de terapia intravenosa en comparación con el método tradicional en instituciones de salud en México. Materiales y Métodos: Estudio prospectivo cuasi...

  3. Tendencias actuales y futuras de la terapia de familias

    Directory of Open Access Journals (Sweden)

    Francisco Almagro Domínguez

    1996-06-01

    Full Text Available Se exponen brevemente la situación actual y las perspectivas de la terapia familiar a partir de la información obtenida en el Séptimo Congreso de IFTA (International Family Therapy Association, celebrado en Guadalajara, México, en Octubre de 1995. Los criterios actuales abarcan desde el ciclo vital y las técnicas nuevas, hasta el tratamiento familiar del SIDA y el cáncer. El futuro se considera, en general, promisorio para estas terapias. Se habla de relacionar diagnósticos, enseñanza integrada a planes de estudio y entrenamiento de personal comunitario; todo ello como perspectiva de la terapia de familiasThe present situation and the outlooks of family therapy based on the information obtained at the 7th Congress of the International Family Therapy Association, held in Guadalajara, Mexico, in October, 1995, are briefly explained. New criteria embrace from the vital cycle and the new techniques to the family treatment of AIDS and cancer. In general, the future is considered as promising for these therapies. It is talked about relating diagnoses, integrating teaching to curricula, and training community personal; all of it as a prospect of family therapy.

  4. Periodontal status and Prevotella intermedia antibody in acute coronary syndrome.

    Science.gov (United States)

    Soejima, Hirofumi; Oe, Yoko; Nakayama, Hideki; Matsuo, Katsuhiko; Fukunaga, Takashi; Sugamura, Koichi; Kawano, Hiroaki; Sugiyama, Seigo; Shinohara, Masanori; Izumi, Yuichi; Ogawa, Hisao

    2009-11-12

    We performed periodontal examination and measured serum antibody levels against Prevotella intermedia in patients with acute coronary syndrome (ACS). Composite periodontal risk scores were significantly higher in the ACS group than in the coronary artery disease (CAD) group. Serum antibody levels were higher in the ACS group than in the CAD group and those were significantly correlated with the composite periodontal risk scores. These results provided important information about the status of P. intermedia infection in patients with ACS.

  5. Antibacterial and antibiofilm effects of iron chelators against Prevotella intermedia.

    Science.gov (United States)

    Moon, Ji-Hoi; Kim, Cheul; Lee, Hee-Su; Kim, Sung-Woon; Lee, Jin-Yong

    2013-09-01

    Prevotella intermedia, a major periodontopathogen, has been shown to be resistant to many antibiotics. In the present study, we examined the effect of the FDA-approved iron chelators deferoxamine (DFO) and deferasirox (DFRA) against planktonic and biofilm cells of P. intermedia in order to evaluate the possibility of using these iron chelators as alternative control agents against P. intermedia. DFRA showed strong antimicrobial activity (MIC and MBC values of 0.16 mg ml(-1)) against planktonic P. intermedia. At subMICs, DFRA partially inhibited the bacterial growth and considerably prolonged the bacterial doubling time. DFO was unable to completely inhibit the bacterial growth in the concentration range tested and was not bactericidal. Crystal violet binding assay for the assessment of biofilm formation by P. intermedia showed that DFRA significantly decreased the biofilm-forming activity as well as the biofilm formation, while DFO was less effective. DFRA was chosen for further study. In the ATP-bioluminescent assay, which reflects viable cell counts, subMICs of DFRA significantly decreased the bioactivity of biofilms in a concentration-dependent manner. Under the scanning electron microscope, P. intermedia cells in DFRA-treated biofilm were significantly elongated compared to those in untreated biofilm. Further experiments are necessary to show that iron chelators may be used as a therapeutic agent for periodontal disease.

  6. Invasion of Human Oral Epithelial Cells by Prevotella intermedia

    Science.gov (United States)

    Dorn, Brian R.; Leung, K.-P.; Progulske-Fox, Ann

    1998-01-01

    Invasion of oral epithelial cells by pathogenic oral bacteria may represent an important virulence factor in the progression of periodontal disease. Here we report that a clinical isolate of Prevotella intermedia, strain 17, was found to invade a human oral epithelial cell line (KB), whereas P. intermedia 27, another clinical isolate, and P. intermedia 25611, the type strain, were not found to invade the cell line. Invasion was quantified by the recovery of viable bacteria following a standard antibiotic protection assay and observed by electron microscopy. Cytochalasin D, cycloheximide, monodansylcadaverine, and low temperature (4°C) inhibited the internalization of P. intermedia 17. Antibodies raised against P. intermedia type C fimbriae and against whole cells inhibited invasion, but the anti-type-C-fimbria antibody inhibited invasion to a greater extent than the anti-whole-cell antibody. This work provides evidence that at least one strain of P. intermedia can invade an oral epithelial cell line and that the type C fimbriae and a cytoskeletal rearrangement are required for this invasion. PMID:9826397

  7. Prevotella intermedia induces prostaglandin E2 via multiple signaling pathways.

    Science.gov (United States)

    Guan, S-M; Fu, S-M; He, J-J; Zhang, M

    2011-01-01

    Prostaglandin E(2) (PGE(2)) plays important roles in the bone resorption of inflammatory diseases such as rheumatoid arthritis and periodontitis via specific prostaglandin receptors (i.e., EP1-EP4). In this study, the authors examined whether Prevotella intermedia regulates PGE(2) production and EP expression in human periodontal ligament fibroblasts (hPDLs); they also explored the potential signaling pathways involved in PGE(2) production. P. intermedia induced PGE(2) production and cyclooxygenase-2 (COX-2) expression in a dose- and time-dependent manner. Indomethacin and NS-398 completely abrogated the P. intermedia-induced PGE(2) production without modulating COX-2 expression. Specific inhibitors of extracellular signal-regulated kinase, c-Jun N-terminal kinase, p38, phosphatidylinositol 3-kinase, and protein kinase C--but not c-AMP and protein kinase A--significantly attenuated the P. intermedia-induced COX-2 and PGE(2) expression. P. intermedia reduced EP1 expression in a concentration- and time-dependent manner. The results indicate that the COX-2-dependent induction of PGE(2) by P. intermedia in hPDLs is mediated by multiple signaling pathways.

  8. Terapia ocupacional en medicina paliativa

    OpenAIRE

    Nicora, Yamila

    2009-01-01

    Terapia ocupacional interviene en el tratamiento de pacientes desde muy temprana edad (recién nacidos) hasta ancianos con déficit en el desempeño de alguna o todas las áreas ocupacionales: - Actividades de la vida diaria (AVD) básicas y personales - AVDI (instrumentales) - Educación - Trabajo - Juego - Ocio - Participación Social Se tienen en cuenta las habilidades de desempeño (motoras, de procesamiento...

  9. Beneficios de la terapia con resistómetro inspiratorio en los pacientes con esclerosis multiple frente a terapias respiratorias convencionales

    OpenAIRE

    Martín Sánchez, Carlos

    2016-01-01

    [ES] La Universidad de Salamanca (USAL) a través del programa de Doctorado en Oncología Clínica realiza un estudio con ASPRODES, la Asociación Salmantina de Esclerosis Múltiple (ASDEM) y la Asociación Zamorana de Esclerosis Múltiple (AZDEM) para llevar a cabo un estudio experimental con el fin de analizar las ventajas que la terapia respiratoria con resistómetro inspiratorio puede aportar a los pacientes con Esclerosis Múltiple frente a las técnicas de fisioterapia respiratoria convencional. ...

  10. La tendencia ambiental urbana en ciudades intermedias. Una propuesta metodológica

    Directory of Open Access Journals (Sweden)

    Pinzón Botero María Victoria

    2011-11-01

    Full Text Available Desde las últim as décadas, es evidente la preocupación m undial por los im pactos ambientales negativos que ponen en riesgo la sostenibilidad urbana y su calidad ambiental. Diferentes estudios exponen las variables más importantes para minim izar estos impactos ambientales, y en este sentido, este trabajo de investigación muestra cómo a partir de un diseño metodológico más sencillo con 27 variables am bientales estratégicas, es posible evaluar la tendencia am biental urbana de ciudades intermedias. Este grupo priorizado de variables trabajadas en el marco de una gestión compartida, orientará el desarrollo de la ciudad hacia una estructura territorial más sostenible, como reto para la planificación ambiental actual y con visión de futuro.

  11. Terapia guiada por objetivos en la resucitación del shock séptico

    OpenAIRE

    Paz Martín, Daniel

    2017-01-01

    Tras la publicación de un meta-análisis de los investigadores del grupo PRISM sobre Terapia Guiada por Objetivos en la Resucitación del Shock Séptico, presentamos esta lectura crítica del principal trabajo realizado a nivel europeo. En el estudio PROMISE, no se demostró beneficio del manejo protocolarizado de la sepsis frente a la práctica clínica habitual.

  12. Terapia nutricional e sepse neonatal

    OpenAIRE

    Freitas,Brunnella Alcantara Chagas de; Leão,Renata Teixeira; Gomes,Andréia Patrícia; Siqueira-Batista,Rodrigo

    2011-01-01

    O objetivo do presente artigo é revisar a literatura acerca dos conhecimentos atuais relativos à terapia nutricional - enteral e parenteral - para os recém-nascidos pré-termo, principalmente os de muito baixo peso, destacando seu efeito protetor na sepse neonatal e na enterocolite necrosante. As diferentes modalidades de alimentação do recém-nascido prematuro - especialmente para aqueles de muito baixo peso - e seu efeito protetor na diminuição de complicações (mormente as infecciosas) foram ...

  13. Relación empírica entre la Terapia Racional Emotivo-Conductual (TREC) de Ellis y la Terapia Cognitiva (TC) de Beck en una muestra costarricense

    OpenAIRE

    Cabezas Pizarro, Hannia; Lega, Leonor I.

    2012-01-01

    En este estudio se explora una posible relación empírica entre la terapia racional emotivoconductual (TREC) de Ellis y la terapia cognitiva (TC) de Beck, dos de los modelos más conspicuos en el campo de la psicoterapia cognitiva actual. Si bien este modelo utiliza el término “cognitivo” en forma tal que se encuentra asociado con el afecto, la fisiología y la conducta (Dobson, 1986), sostiene que las perturbaciones emocionales, en esencia, son causadas por el proceso cognitivo del individuo. E...

  14. Cuanta más psicología, mejor: eficacia para dejar de fumar de la terapia cognitiva conductual intensiva y de los parches de nicotina combinados con terapia cognitiva conductual intensiva y menos intensiva

    OpenAIRE

    Fernández Arias, Ignacio Gabino; García Vera, María Paz; Sanz Fernández, Jesús

    2014-01-01

    Para comparar la eficacia para dejar de fumar de la terapia cognitiva conductual intensiva (TCC/I), la TCC/I con parches de nicotina (TCC/I+PN) y la terapia cognitiva conductual no intensiva con parches de nicotina (TCC/NI+PN) se realizó un estudio experimental de medidas repetidas con 235 fumadores adultos asignados aleatoriamente a uno de esos tres tratamientos. Entre los pacientes que completaron el tratamiento (n = 152) la TCC/I+PN mostró tasas de abstinencia a los 6 meses y al año, mayor...

  15. Síndrome del "estudiante quemado"en alumnos de Terapia Ocupacional.

    OpenAIRE

    Juy Martín, Rubén

    2016-01-01

    [ES]En el ámbito escolar nos encontramos, frecuentemente, con compañeros que nos confiesan estar muy estresados y que han pensado en abandonar los estudios porque no pueden más. En el presente TFG se realiza un estudio para conocer cómo afecta “El Síndrome del estudiante quemado” a 30 alumnos de cuarto curso de Terapia Ocupacional de la Universidad de Salamanca. Para ello se utilizaron varios cuestionarios: Cuestionario sociodemográfico, la Escala de Cansancio Emocional (ECE) y la Escala d...

  16. Irrigación subgingival com clorhexidina en terapia periodontal no quirúrgica

    OpenAIRE

    Rösing, Cassiano Kuchenbecker; Toledo, Benedicto Egbert Correa de

    2009-01-01

    El objetivo del siguiente estudio es evaluar los efectos clínicos de la irrigación profesional subgingival con Clorhexidina como terapia adjunta a los procedimientos de raspado y alisado radicular. Este estudio fue desarrollado en 17 pacientes con cuatro bolsas periodontales de 6 mm o más de profundidad, los pacientes fueron divididos en cuatro grupos: solo raspado y alisado radicular; raspaje y alisado radicular más irrigación con solución salina; raspado y alisado radicular más irrigación c...

  17. Thalassemia intermedia: An overview | Shawky | Egyptian Journal of ...

    African Journals Online (AJOL)

    β-Thalassemia is considered the most common chronic hemolytic anemia in Egypt. Patients with β-thalassemia whose anemia is not so severe as to necessitate regular transfusions are said to have thalassemia intermedia. It is characterized by a significant genetic and clinical heterogeneity. The clinical phenotype ranges ...

  18. Irradiation for progressive paraparesis in β-thalassemia intermedia

    International Nuclear Information System (INIS)

    Wilson, J.J.; Hill, M.E.; Crookston, J.H.

    1980-01-01

    This article describes the case history of a patient suffering from β-thalassemia intermedia. The patient was treated with radiation to the thoraic spine (1500 rad in 5 days) and his condition improved. Subsequent tomography indicated the success of this type of irradiation without recourse to laminectomy. (T.I.)

  19. Nucleases from Prevotella intermedia can degrade neutrophil extracellular traps.

    Science.gov (United States)

    Doke, M; Fukamachi, H; Morisaki, H; Arimoto, T; Kataoka, H; Kuwata, H

    2017-08-01

    Periodontitis is an inflammatory disease caused by periodontal bacteria in subgingival plaque. These bacteria are able to colonize the periodontal region by evading the host immune response. Neutrophils, the host's first line of defense against infection, use various strategies to kill invading pathogens, including neutrophil extracellular traps (NETs). These are extracellular net-like fibers comprising DNA and antimicrobial components such as histones, LL-37, defensins, myeloperoxidase, and neutrophil elastase from neutrophils that disarm and kill bacteria extracellularly. Bacterial nuclease degrades the NETs to escape NET killing. It has now been shown that extracellular nucleases enable bacteria to evade this host antimicrobial mechanism, leading to increased pathogenicity. Here, we compared the DNA degradation activity of major Gram-negative periodontopathogenic bacteria, Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans. We found that Pr. intermedia showed the highest DNA degradation activity. A genome search of Pr. intermedia revealed the presence of two genes, nucA and nucD, putatively encoding secreted nucleases, although their enzymatic and biological activities are unknown. We cloned nucA- and nucD-encoding nucleases from Pr. intermedia ATCC 25611 and characterized their gene products. Recombinant NucA and NucD digested DNA and RNA, which required both Mg 2+ and Ca 2+ for optimal activity. In addition, NucA and NucD were able to degrade the DNA matrix comprising NETs. © 2016 The Authors Molecular Oral Microbiology Published by John Wiley & Sons Ltd.

  20. Efecto de la aplicación de terapia conductual en la adquisición de destrezas básicas para el aprendizaje, en tres niños con autismoa partir de la expresión artística

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    Hannia Cabezas Pizarro

    2004-01-01

    Full Text Available El presente estudio muestra el efecto de la utilización de terapia conductual aplicada, en tres niños con autismo, y cuyo propósito fue el de desarrollar aquellas conductas de entrada básicas para el aprendizaje como son el permanecer sentados, el control de llanto, el establecimiento de contacto ocular y la identificación del yo, consideradas habilidades básicas que deben tener los niños para incursionar en el proceso educativo. Una vez que el niño adquiere esta gama de habilidades, estos repertorios conductuales se convierten a su vez en básicos para destrezas más complejas. Siguiendo este modelo, se trabajó una segunda área que se denominó conducta intermedia, que requiere de las habilidades previamente establecidas, y dentro de las que se citan el seguimiento de instrucciones, la discriminación de objetos y la identificación de las partes del cuerpo, a su vez este repertorio conductual se convirtió en básico para desarrollar habilidades más complejas como el reconocimiento de colores, de modo que unas conductas se convierten en requisitos de otras, hasta llevar a término un programa de intervención educativo. El proceso de intervención educativa, contempló una gama amplia de habilidades, sin embargo para efectos de este estudio, se seleccionó una muestra representativa de cada área

  1. Menopausia y terapia hormonal de reemplazo

    OpenAIRE

    Cobo, Edgard; Fundación Valle de Lili

    1996-01-01

    La terapia hormonal en la menopausia/ menopausia y terapia hormonal de reemplazo (THR)/¿Qué es la menopausia?/ ¿Porqué hay tanto “ruido” acerca de la menopausia, si es un evento natural en la vida de toda mujer?/ ¿Qué significa terapia hormonal de reemplazo?(THR)/ ¿Cuáles son las ventajas de recibir la THR?/ Mejoraría en la calidad de vida/ Prevención de enfermedad/ ¿Quiere esto decir que absolutamente todas las mujeres deber recibir una THR?/ ¿Cuáles son las molestias más frecuentes a las qu...

  2. β-Thalassemia Intermedia: A Bird’s-Eye View

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    Anthony Haddad

    2014-03-01

    Full Text Available Beta-thalassemia is due to a defect in the synthesis of the beta-globin chains, leading to alpha/beta imbalance, ineffective erythropoiesis, and chronic anemia. The spectrum of thalassemias is wide, with one end comprising thalassemia minor, which consists of a mild hypochromic microcytic anemia with no obvious clinical manifestations, while on the other end is thalassemia major, characterized by patients who present in their first years of life with profound anemia and regular transfusion requirements for survival. Along the spectrum lies thalassemia intermedia, a term developed to describe patients with manifestations that are neither mild enough nor severe enough to be classified in the spectrum’s extremes. Over the past decade, our understanding of β-thalassemia intermedia has increased tremendously with regards to molecular information as well as pathophysiology. It is now clear that β-thalassemia intermedia has a clinical presentation as well as complications associated with the disease that are different from those of β-thalassemia major. This review is designed to tackle issues related to β-thalassemia intermedia from the basic definition of the disease to paramedical issues, namely the quality of life in these patients. Genetics and pathophysiology are revisited, as well as the complications specific to this disease. These complications include effects on several organ systems, including the cardiovascular, hepatic, endocrine, renal, brain, and skeletal systems. Extramedullary hematopoiesis is also discussed in this article. Risk factors are highlighted and cutoffs are identified to minimize morbidities in β-thalassemia intermedia. Several treatment modalities are considered by shining a light on the pros and cons of each modality, as well as the role of special pharmacological agents in the progress of the disease and its morbidities. Finally, health-related quality of life is discussed in these patients with a direct comparison

  3. HACIA UN CURRÍCULO FLEXIBLE: SALIDAS INTERMEDIAS Y CURSOS OPTATIVOS

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    Carlos Ortigoza Garcell

    2013-04-01

    Full Text Available Tradicionalmente los currículos de las carreras universitarias se han caracterizado por el predominio de una formación de tipo terminal con una sola salida al mercado laboral. Con el fin de contribuir de manera flexible desde la Formación Laboral y Profesional a que los estudiantes definan su trayectoria formativa, en este trabajo se analizan las ventajas de las salidas intermedias en una carrera universitaria, sin afectar la continuidad de los estudios superiores ni la calidad en la formación del egresado universitario. Estas permiten a los estudiantes obtener un título de nivel medio que los pone en condiciones de salir al mercado laboral con un perfil ocupacional relacionado con la carrera universitaria que estudia. Desde la perspectiva curricular la propuesta realizada se basa en currículos flexibles con salidas intermedias donde pueden ser de gran utilidad los cursos optativos y/o electivos.PALABRAS CLAVE: currículo; salidas intermedias; asignaturas optativasTOWARDS A FLEXIBLE CURRICULUM: INTERMEDIATE EXITS AND OPTIONAL COURSES ABSTRACTTraditionally, the curricula of the university degree courses have been focused on the formation of professionals within the required period for their graduation (5-6 years, with a single exit to the labour market. This research is aimed at contributing, from the Labour and Professional Formation of the students, to the definition of their formative process, according to their needs and interests. This paper also analyses the advantages of the intermediate exits in a university degree course, and how they allow students to obtain a college degree without affecting the continuation of the higher education studies or the quality of the formation of the university graduate. Such reality favours the insertion of the students in the labour market under conditions that suit the qualifications and experience related to the degree they are doing. From the curricular perspective, the proposal is based on

  4. Prevotella intermedia ATCC 25611 targets host cell lamellipodia in epithelial cell adhesion and invasion.

    Science.gov (United States)

    Gursoy, U K; Könönen, E; Uitto, V-J

    2009-08-01

    The Prevotella intermedia group bacteria, namely P. intermedia, Prevotella nigrescens, and Prevotella pallens, are phylogenetically closely related and potentially connected with oral and gastrointestinal tract disease pathogenesis. The aim of the present study was to examine whether these species differ in their capabilities of adhesion to and invasion of epithelial cells. Adhesion and invasion were assayed by standard antibiotic/culture assays and fluorescent microscopy techniques. The effect of Prevotella strains on epithelial cell viability was measured using a commercial cell proliferation assay. The strains P. intermedia ATCC 25611 and P. nigrescens ATCC 33263 adhered to epithelial cells, the adhesion numbers of P. intermedia being twice as high as those of P. nigrescens. These strains invaded epithelial cells but invasion was weak. The adhesion of P. intermedia was specifically targeted to epithelial cell lamellipodia. The number of adhered P. intermedia cells increased or decreased when the formation of lamellipodia was stimulated or inhibited, respectively. None of the tested strains showed toxic effects on epithelial cells; a clinical P. intermedia strain even increased the number of viable cells by about 20%. The results suggest that among the P. intermedia group bacteria, P. intermedia and P. nigrescens type strains can adhere to and invade epithelial cells, the capability of P. intermedia ATCC 25611(T) being highest in this context. This strain proved to have a special affinity in binding to epithelial cell lamellipodia.

  5. Depression prevalence in Intensive Care Unit nursing workers: a study at hospitals in a northwestern city of São Paulo State Prevalencia de depresión en trabajadores de enfermería de Unidades de Terapia Intensiva: estudio en hospitales de una ciudad del noroeste del Estado de Sao Paulo Prevalência de depressão em trabalhadores de enfermagem de Unidade de Terapia Intensiva: estudo em hospitais de uma cidade do noroeste do Estado São Paulo

    Directory of Open Access Journals (Sweden)

    Divane de Vargas

    2011-10-01

    Full Text Available This study aimed to estimate the prevalence of depression in nursing staff working in Intensive Care Units of hospitals from a city in Northwestern São Paulo State - Brazil, examining its association with participants' socio-demographic characteristics. The Beck Depression Inventory was applied to a sample of 67 nursing workers from three general hospitals, showing an 28.4% prevalence of depression. The analysis based on the multiple model showed a significant association between depression and marital status (OR=1.52, night work (OR=1.46 and double shifts (OR=2.11. Also, there were significant percentages of workers who reported discouragement, sadness and hopelessness. In conclusion, the prevalence of depression is significant and more attention should be paid to this problem workers at these units face. Further studies are needed in the attempt to broaden knowledge on the subject, which can support strategies to guarantee attention to ICU nursing workers' physical and mental health needs.Este estudio fue realizado con el objetivo de estimar la prevalencia de depresión, en trabajadores de enfermería de Unidades de Terapia Intensiva (UTI de hospitales de una ciudad del Noroeste del estado de Sao Paulo, analizando su asociación con las características sociodemográficas de los participantes. Se aplicó el Inventario de Depresión de Beck a una muestra de 67 trabajadores de enfermería de tres hospitales generales; se observó una prevalencia de 28,4% de depresión. El análisis por el modelo múltiple demostró asociación significativa entre depresión y estado civil (OR=1,52, trabajo nocturno (OR=1,46 y doble jornada (OR=2,11, fueron significativos también los porcentajes de trabajadores que relataron desanimo, tristeza y desesperanza. Se concluye que es significativa la prevalencia de depresión y que se debe dar mayor atención a ese problema enfrentado por los trabajadores de esas unidades. Se sugiere la realización de otros estudios

  6. CLINICAL APPLICATION OF RECOMBINANT ERYTHROPOIETIN IN BETA-THALASSAEMIA INTERMEDIA.

    Science.gov (United States)

    Asadov, Ch; Alimirzoyeva, Z; Hasanova, M; Mammadova, T; Shirinova, A

    2016-06-01

    Research objective is to study the efficacy of recombinant erythropoietin (epoetin alfa) as alternative method of treatment beta-thalassemia intermedia. Study involved 58 patients with beta-thalassemia intermedia (23 women and 35 men). In all observed patients was defined levels of hemoglobin (Hb), red blood cells (RBC), erythrocyte indexes (MCV, MCH, MCHC), hemoglobin fractions (HbA, HbA2, HbF), serum ferritin, serum erythropoietin before and after administrated rEPO. All patients received rEPO during 6 month at the dose - 10000 IU subcutaneously. The majority of patients - 39 (67%) had a good response to rEPO (increase in hemoglobin level more than 20 g/l); 16 patients (28%) had a mean response (increase in Hb 10 - 20 g/l); in 3 (5%) patients occurred poor response to rEPO therapy (increase in Hb beta-thalassemia intermedia patients there was a statistically significant change in the number of RBC, levels of HbF and sEPO. The evaluation of interdependence between the indices of the baseline sEPO and increased Hb values in patients after rEPO treatment revealed the presence of the reverse direct relationship (r=-0.67). Based on the results, it can be concluded that the use of rEPO in complex therapy of beta-thalassemia intermedia leads to increased levels of Hb and consequently reducing the need for blood transfusions, and accordingly expected to prevent severe complications of blood transfusion (alloimmunization, hypersplenism, iron overload, contamination transmissible infections) facilitating normal growth and development, and a better quality of life.

  7. Characterization of L-cysteine desulfhydrase from Prevotella intermedia.

    Science.gov (United States)

    Yano, T; Fukamachi, H; Yamamoto, M; Igarashi, T

    2009-12-01

    Hydrogen sulfide is responsible for lysis of red blood cells and is a major compound for oral malodor. To clarify the production mechanism of hydrogen sulfide in Prevotella intermedia, we found an L-cysteine desulfhydrase gene (lcs) homologue on the genome database of P. intermedia ATCC25611 and characterized its gene product. The lcs gene homologue cloned into pGEX6p-1 vector was expressed in Escherichia coli and purified. Lcs activity was assayed by detection of the reaction products (hydrogen sulfide and pyruvate) or its derivatives from L-cysteine. Site-directed mutagenesis was used to convert an amino acid of the Lcs molecule. The purified lcs gene product catalysed the degradation of L-cysteine to pyruvate, ammonia, and hydrogen sulfide, indicating that the protein is L-cysteine desulfhydrase. The enzyme required pyridoxal 5'-phosphate as a cofactor, and it was highly active at pH 7.0 and completely inhibited by ZnCl(2). The K(m) and V(max) of the enzyme were 0.7 mm and 4.2 micromol/min/mg, respectively. Replacement of Tyr-59, Tyr-118, Asp-198, and Lys-233 with any of the amino acids resulted in the complete disappearance of Lcs activity, implying that these amino acids are essential for enzyme activity. In addition, hydrogen sulfide produced by this enzyme lysed sheep red blood cells and modified hemoglobin. These results show the enzymatic properties of L-cysteine desulfhydrase from P. intermedia ATCC25611 and also suggest that the Lcs enzyme, which produces hydrogen sulfide from L-cysteine, is closely associated with the pathogenesis of P. intermedia.

  8. Pigment Production by the Edible Filamentous Fungus Neurospora Intermedia

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    Rebecca Gmoser

    2018-02-01

    Full Text Available The production of pigments by edible filamentous fungi is gaining attention as a result of the increased interest in natural sources with added functionality in the food, feed, cosmetic, pharmaceutical and textile industries. The filamentous fungus Neurospora intermedia, used for production of the Indonesian food “oncom”, is one potential source of pigments. The objective of the study was to evaluate the fungus’ pigment production. The joint effect from different factors (carbon and nitrogen source, ZnCl2, MgCl2 and MnCl2 on pigment production by N. intermedia is reported for the first time. The scale-up to 4.5 L bubble column bioreactors was also performed to investigate the effect of pH and aeration. Pigment production of the fungus was successfully manipulated by varying several factors. The results showed that the formation of pigments was strongly influenced by light, carbon, pH, the co-factor Zn2+ and first- to fourth-order interactions between factors. The highest pigmentation (1.19 ± 0.08 mg carotenoids/g dry weight biomass was achieved in a bubble column reactor. This study provides important insights into pigmentation of this biotechnologically important fungus and lays a foundation for future utilizations of N. intermedia for pigment production.

  9. Cryopreservation of Byrsonima intermedia embryos followed by room temperature thawing

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    Luciano Coutinho Silva

    2014-07-01

    Full Text Available Byrsonima intermedia is a shrub from the Brazilian Cerrado with medicinal properties. The storage of biological material at ultra-low temperatures (-196°C is termed cryopreservation and represents a promising technique for preserving plant diversity. Thawing is a crucial step that follows cryopreservation. The aim of this work was to cryopreserve B. intermedia zygotic embryos and subsequently thaw them at room temperature in a solution rich in sucrose. The embryos were decontaminated and desiccated in a laminar airflow hood for 0-4 hours prior to plunging into liquid nitrogen. The embryo moisture content (% MC during dehydration was assessed. Cryopreserved embryos were thawed in a solution rich in sucrose at room temperature, inoculated in a germination medium and maintained in a growth chamber. After 30 days, the embryo germination was evaluated. No significant differences were observed between the different embryo dehydration times, where they were dehydrated for at least one hour. Embryos with a MC between 34.3 and 20.3% were germinated after cryopreservation. In the absence of dehydration, all embryos died following cryopreservation. We conclude that B. intermedia zygotic embryos can be successfully cryopreserved and thawed at room temperature after at least one hour of dehydration in a laminar airflow bench.

  10. Diseño de nuevos materiales fotovoltaicos de banda intermedia

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    Tablero, C.

    2004-04-01

    Full Text Available A new kind of photovoltaic material with an intermediate band (MIB and semiconductor characteristics have been recently identified in a previous work. These MIB present an isolated half-filled intermediate band located in the semiconductor energy band-gap compare to the conventional materials used to manufacture photovoltaic devices. Quantum efficiencies of photovoltaic conversion of these materials are related to efficient light absorption. In this work we use quantum mechanical techniques to design this type of materials, specifically the Ga4P3Ti and Ga4As3Ti alloys systems. For this task we use an accurate first principles methods to calculate band diagram and optical material parameters. Density Functional Theory has been used with the semi-local generalized gradient approximation (GGA correction for the exchange-correlation potential, norm conserving non-local pseudopotential and confined lineal combination of atomic pseudo-orbitals. In order to further evaluate the potential of such materials of their light-absorption properties, direct optical transitions have been also determined using for that, the aforementioned first principles calculations.

    En trabajos recientes se han descrito una serie de nuevos materiales conocidos como materiales de banda intermedia (MIB que presentan una banda intermedia, aislada y parcialmente llena respecto a los semiconductores utilizados actualmente en la fabricación de dispositivos fotovoltaicos. Estas características hacen de este tipo de nuevos materiales candidatos para aumentar significativamente la absorción efectiva de la luz solar. En este trabajo utilizamos técnicas mecanocuánticas para el diseño y estudio de este tipo de materiales, en particular los sistemas Ga4P3Ti y Ga4As3Ti. En primer lugar analizamos las características estructurales y las propiedades electrónicas utilizando los diagramas de dispersión de energía. Para este estudio utilizamos el método DFT (Density Functional

  11. Susceptibilities of Eikenella corrodens, Prevotella intermedia, and Prevotella nigrescens Clinical Isolates to Amoxicillin and Tetracycline

    OpenAIRE

    Luong, N.; Tsai, J.; Chen, C.

    2001-01-01

    The AB Biodisk Etest showed that 106 (100%) and 98 (92%) isolates of Eikenella corrodens were susceptible to amoxicillin and tetracycline, respectively. Twenty-three (68%) Prevotella intermedia isolates and 14 (67%) Prevotella nigrescens isolates were susceptible to amoxicillin. Seventy-nine percent of the P. intermedia isolates and 67% of the P. nigrescens isolates were susceptible to tetracycline. A higher percentage of β-lactamase-producing isolates of P. intermedia and P. nigrescens were ...

  12. Development and evaluation of new primers for PCR-based identification of Prevotella intermedia.

    Science.gov (United States)

    Zhou, Yanbin; Liu, Dali; Wang, Yiwei; Zhu, Cailian; Liang, Jingping; Shu, Rong

    2014-08-01

    The aim of this study was to develop new Prevotella intermedia-specific PCR primers based on the 16S rRNA. The new primer set, Pi-192 and Pi-468, increased the accuracy of PCR-based P. intermedia identification and could be useful in the detection of P. intermedia as well as epidemiological studies on periodontal disease. Copyright © 2014 Elsevier Ltd. All rights reserved.

  13. NUEVAS TERAPIAS EN EL MANEJO DE LOS GLIOMAS DE ALTO GRADO

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    Dr. Raúl Valenzuela

    2017-05-01

    Full Text Available El reto de mejorar el tratamiento actual de los gliomas de alto grado ha sido abordado con inmensa creatividad, desarrollándose una gran variedad de estrategias para este fin. El análisis interino de un estudio fase III mostró que el beneficio de la terapia standard mejora al complementarla con terapia de campos eléctricos alternantes (Novo TTF-100 A; dadas las limitaciones de un análisis interino, queda por establecer la real magnitud y relevancia clínica de esta mejoría. El presunto beneficio de usar tratamiento antiviral contra citomegalovirus es muy controvertido y su utilidad no se ha demostrado en forma fehaciente. Las terapias antiangiogénicas han mostrado ser útiles para el manejo de las recurrencias, al menos como tratamiento sintomático, pero no han demostrado mejorar la sobrevida en pacientes de novo. La terapia dirigida contra señales intracelulares de crecimiento, conceptualmente muy atractiva, todavía no ha logrado resultados clínicos exitosos. Hay datos preliminares promisorios respecto al uso clínico de vacunas antitumorales y de inmunomodulación con inhibidores de “checkpoints”; también hay algunos datos preliminares a favor del uso de virus oncolíticos. La terapia genética parece estar en etapas aún muy tempranas de su desarrollo y probablemente demore más tiempo en llegar a demostrar utilidad clínica.

  14. Terapia inmunosupresora en pacientes con Nefropatía Membranosa Idiopática

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    Giovanna Llerena García

    2009-04-01

    Full Text Available Objetivo: Evaluar la respuesta a la terapia combinada según el esquema de Ponticelli en pacientes con Nefropatía Membranosa (NM idiopática, en la tasa de remisión de la proteinuria, la tasa de filtración glomerular (TFG y la frecuencia de complicaciones. Material y métodos: Estudio observacional descriptivo tipo serie de casos en pacientes con NM idiopàtica durante los años 1995 - 2005 que recibieron terapia inmunosupresora, según esquema de Ponticelli. Se determinó la tasa de remisión de la proteinuria y la evolución de la TFG. Resultados: Diez pacientes con NM idiopática cumplieron los criterios de inclusión. La mitad de los casos tuvo remisión parcial (RP al finalizar la terapia; no hubo casos con remisión completa (RC. Al último control, 10% presentó RC y 70% RP. El 80% de los pacientes presentó efectos adversos. Conclusiones: Al finalizar la terapia inmunosupresora según esquema de Ponticelli, se evidencia reducción de la proteinuria (p>0,05, y remisión al último control (p < 0,05, probablemente asociado al uso de bloqueadores del eje renina-angiotensina-aldosterona. Los efectos adversos asociados a la terapia inmunosupresora son frecuentes, sin embargo ningún paciente descontinuó el tratamiento. (Rev Med Hered 2009;20:60-69.

  15. Costo-beneficio de la terapia en ambiente húmedo versus terapia tradicional: el caso de un paciente con úlcera en la extremidad inferior

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    Blanca Lilia Reyes-Rocha

    Full Text Available Actualmente, ha cobrado relevancia el uso de la terapia húmeda en la cura de las heridas crónicas; sin embargo, persiste el uso de la terapia tradicional como técnica para lograr la cicatrización de las mismas. El caso que presentamos tiene como propósito determinar el costo-beneficio del empleo de apósitos de cura en ambiente húmedo versus cura tradicional en un paciente con úlcera en la extremidad inferior. Los resultados muestran que el coste es mayor con la terapia en ambiente húmedo; sin embargo, el periodo de cicatrización de la herida es menor en comparación con el tiempo de cicatrización que conlleva la cura tradicional; esto redunda en un beneficio para el paciente al alcanzar su recuperación en un menor tiempo. Estos hallazgos permiten proponer hipótesis para estudios posteriores bajo otro diseño metodológico, a fin de generar evidencia científica que coadyuve en la mejora de los procesos de atención en beneficio de la población con este tipo de padecimiento.

  16. Terapia nutricional e sepse neonatal

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    Brunnella Alcantara Chagas de Freitas

    2011-12-01

    Full Text Available O objetivo do presente artigo é revisar a literatura acerca dos conhecimentos atuais relativos à terapia nutricional - enteral e parenteral - para os recém-nascidos pré-termo, principalmente os de muito baixo peso, destacando seu efeito protetor na sepse neonatal e na enterocolite necrosante. As diferentes modalidades de alimentação do recém-nascido prematuro - especialmente para aqueles de muito baixo peso - e seu efeito protetor na diminuição de complicações (mormente as infecciosas foram analisadas. A utilização preferencial do leite materno na nutrição enteral, o controle das ofertas energético-protéicas, o início precoce da nutrição enteral mínima, a introdução precoce da alimentação parenteral - nas primeiras 24 horas - e a utilização dos imunonutrientes que tenham estudos suficientes para fundamentar sua indicação podem se constituir em boas diretrizes adjuvantes na prevenção da sepse neonatal e da enterocolite necrosante. Sem embargo, percebe-se a necessidade de mais estudos - preferencialmente multicêntricos, controlados e randomizados - para esclarecer o papel protetor da nutrição no RNPT, não somente na prevenção de infecções, mas também para auxiliar o desenvolvimento neural e a prevenção de consequências deletérias futuras.

  17. V Jornadas de actualización en terapia ocupacional: Formación continuada en terapia ocupacional

    OpenAIRE

    Asensio, Carmen; García Lemos, C.; Viedma, María José de; Pérez Corrales, Jorge; Sánchez Cabeza, Ángel; Pascual Agudo, Elisa; Santos, Francisco de los

    2014-01-01

    V Jornadas de actualización en terapia ocupacional: Formación continuada en terapia ocupacional, celebradas en la Universidad Rey Juan Carlos campus de Alcorcón en 2014. Fisioterapia, Terapia Ocupacional, Rehabilitación y Medicina Física

  18. Terapia cognitivo-comportamental da fobia social

    OpenAIRE

    Ito,Lígia M; Roso,Miréia C; Tiwari,Shilpee; Kendall,Philip C; Asbahr,Fernando R

    2008-01-01

    OBJETIVO: Este artigo revisa aspectos relevantes da fobia social e os estágios de tratamento através da terapia cognitivo-comportamental em crianças, adolescentes e adultos. MÉTODO: A partir do banco de dados Medline, realizou-se revisão da literatura publicada a respeito do tratamento da fobia social por meio da terapia cognitivo-comportamental. RESULTADOS: Revisão da literatura sugere que a fobia social é uma condição prevalente e crônica, caracterizada por inibição social e timidez excessi...

  19. Uveítis intermedia: Experiencias terapéuticas Intermediate uveitis: Therapeutic experiences

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    Sandra M. Borges Pérez

    2001-12-01

    Full Text Available Se realizó un estudio descriptivo prospectivo a 50 ojos (41 pacientes con el diagnóstico de uveítis intermedia durante un período de 3 años. Esta patología fue más frecuente en la mujer (58 %, en edades jóvenes (25 a 45 años y en plena actividad laboral. Se presentan complicaciones y dentro de ellas el edema macular cistoide constituye la causa que más afecta la visión. La mejoría de la agudeza visual fue más notable en los pacientes bajo régimen de tratamiento médico acompañado de tratamiento quirúrgico (40 %. El 50 % de los pacientes presentaron recidivas.A descriptive and prospective study of 50 eyes (41 patients with the diagnosis of intermediate uveitis was conducted during 3 years. This pathology was more frequent in women (58 %, in young patients (25-45 years old and among workers. Among the complications, the cystoid macular edema was the main cause of vision affections. The improvement of visual acuity was more remarkable in patients under medical and surgical treatment (40 %. 50 % of the patients had relapses.

  20. SYNCHROTRON X-RAY ABSORPTION-EDGE COMPUTED MICROTOMOGRAPHY IMAGING OF THALLIUM COMPARTMENTALIZATION IN IBERIS INTERMEDIA

    Science.gov (United States)

    Thallium (TI) is an extremely toxic metal which, due to its similarities to K, is readily taken up by plants. Thallium is efficiently hyperaccumulated in Iberis intermedia as TI(I). Distribution and compartmentalization of TI in I. intermedia is highes...

  1. The identification of genes specific to Prevotella intermedia and Prevotella nigrescens using genomic subtractive hybridization.

    Science.gov (United States)

    Masakiyo, Yoshiaki; Yoshida, Akihiro; Shintani, Yasuyuki; Takahashi, Yusuke; Ansai, Toshihiro; Takehara, Tadamichi

    2010-06-01

    Prevotella intermedia and Prevotella nigrescens, which are often isolated from periodontal sites, were once considered two different genotypes of P. intermedia. Although the genomic sequence of P. intermedia was determined recently, little is known about the genetic differences between P. intermedia and P. nigrescens. The subtractive hybridization technique is a powerful method for generating a set of DNA fragments differing between two closely related bacterial strains or species. We used subtractive hybridization to identify the DNA regions specific to P. intermedia ATCC 25611 and P. nigrescens ATCC 25261. Using this method, four P. intermedia ATCC 25611-specific and three P. nigrescens ATCC 25261-specific regions were determined. From the species-specific regions, insertion sequence (IS) elements were isolated for P. intermedia. IS elements play an important role in the pathogenicity of bacteria. For the P. intermedia-specific regions, the genes adenine-specific DNA-methyltransferase and 8-amino-7-oxononanoate synthase were isolated. The P. nigrescens-specific region contained a Flavobacterium psychrophilum SprA homologue, a cell-surface protein involved in gliding motility, Prevotella melaninogenica ATCC 25845 glutathione peroxide, and Porphyromonas gingivalis ATCC 33277 leucyl-tRNA synthetase. The results demonstrate that the subtractive hybridization technique was useful for distinguishing between the two closely related species. Furthermore, this technique will contribute to our understanding of the virulence of these species. 2009 Elsevier Ltd. All rights reserved.

  2. Chair-side detection of Prevotella Intermedia in mature dental plaque by its fluorescence.

    Science.gov (United States)

    Nomura, Yoshiaki; Takeuchi, Hiroaki; Okamoto, Masaaki; Sogabe, Kaoru; Okada, Ayako; Hanada, Nobuhiro

    2017-06-01

    Prevotella intermedia/nigrescens is one of the well-known pathogens causing periodontal diseases, and the red florescence excited by the visible blue light caused by the protoporphyrin IX in the bacterial cells could be useful for the chair-side detection. The aim of this study was to evaluated levels of periodontal pathogen, especially P. intermedia in clinical samples of red fluorescent dental plaque. Thirty two supra gingival plaque samples from six individuals were measured its fluorescence at 640nm wavelength excited by 409nm. Periodontopathic bacteria were counted by the Invader PLUS PCR assay. Co-relations the fluorescence intensity and bacterial counts were analyzed by Person's correlation coefficient and simple and multiple regression analysis. Positive and negative predictive values of the fluorescence intensities for with or without P. intermedia in supragingival plaque was calculated. When relative fluorescence unit (RFU) were logarithmic transformed, statistically significant linear relations between RFU and bacterial counts were obtained for P. intermedia, Porphyromonas gingivalis and Tannerella forsythia. By the multiple regression analysis, only P. intermedia had statistically significant co-relation with fluorescence intensities. All of the fluorescent dental plaque contained P. intermedia m. In contrast, 28% of non-fluorescent plaques contained P. intermedia. To check the fluorescence dental plaque in the oral cavity could be the simple chair-side screening of the mature dental plaque before examining the periodontal pathogens especially P. intermedia by the PCR method. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Beta-thalassemia intermedia associated with moyamoya syndrome.

    Science.gov (United States)

    Göksel, Basak Karakurum; Ozdogu, Hakan; Yildirim, Tulin; Oğuzkurt, Levent; Asma, Suheyl

    2010-07-01

    Moyamoya syndrome (MMS) is a progressive disorder. We report a 19-year-old boy with beta-thalassemia who presented with a left hemiparesis. Brain MRI showed old middle cerebral artery and left frontal subcortical white matter infarcts. Brain magnetic resonance angiography and digital subtraction angiography revealed occlusion of the bilateral internal carotid arteries with a rich network of basal collateral vessels. To our knowledge this is the third report of beta-thalassemia intermedia and MMS, and the first report of a patient in Turkey. It emphasizes the potential for cerebral infarct due to anemia, protein S and thrombocytosis.

  4. Terapia de juego desde el Modelo Integrativo aplicada a escolares que presentan conductas agresivas

    OpenAIRE

    Figueroa Siguenza, María Elena

    2013-01-01

    La agresividad infantil es un dificultad a nivel escolar, este estudio busca dar una respuesta a esta problemática en base al diseño de un programa de terapia de juego grupal basada en el Modelo Integrativo Focalizado en la Personalidad. Para ello se utilizó la metodología de análisis de casos en donde se identificó a 7 niños que presentaban conductas agresivas a quienes se les aplicó pruebas diagnósticas y fundamentalmente se evaluó sus rasgos de personalidad considerando su importancia en e...

  5. DANO OCUPACIONAL NA UNIDADE DE TERAPIA INTENSIVA NEONATAL: A PERCEPÇÃO DA ENFERMEIRA

    OpenAIRE

    MARIA ALVANI DIAS PEDROZA; ANTONIA DO CARMO SOARES CAMPOS; MÁRCIA MARIA COELHO OLIVEIRA

    2006-01-01

    Se trata de estudio cuantitativo, exploratorio y descriptivo, con el objetivo de conocer la percepción de las enfermeras con respecto a la influencia del desempeño en la asistencia de enfermería y en el ambiente laboral. Tuvo por escenario la Unidad de Terapia Intensiva Neonatal (UTIN) de una maternidad escuela, en Fortaleza-CE. Recogimos los datos entre agosto y septiembre de 2004 a través de un cuestionario estructurado en parte, aplicado a 24 enfermeras activas. Verificamos que la mayor co...

  6. Relación de conteo de granulocitos inmaduros por citometria de flujo y niveles de procalcitonina como indicadores tempranos de sepsis en pacientes adultos de la unidad de terapia intensiva del Hospital Carlos Andrade Marin. 2011 – 2012

    OpenAIRE

    González Ñato, Rosa Julia; Reinoso Pérez, Karina Gabriela

    2014-01-01

    La sepsis constituye la principal causa de muerte en los pacientes de las Unidades de Terapia Intensiva, es considerada como la respuesta sistémica frente a la infección, en sus formas más severas. Estudios recientes muestran el significado clínico del recuento de Granulocitos inmaduros como un nuevo marcador de la inflamación aguda. Encontrándose que pacientes de las unidades de Terapia Intensiva pueden presentar un aumento de Granulocitos Inmaduros. Se realizó un estudio epidemiológico ana...

  7. Beneficios de la terapia cognitivo-conductual y la presoterapia en pacientes obesos: ensayo clínico aleatorizado Benefits of cognitive behavior therapy and acupressure therapy in obese patients: a randomized clinical trial

    OpenAIRE

    V. Torres; A. Mª Castro Sánchez; G. A. Matarán Peñarocha; I. Lara Palomo; Mª E. Aguilar Ferrándiz; C. Moreno Lorenzo

    2011-01-01

    Objetivo: el objetivo del presente trabajo fue analizar la modificación de los hábitos de vida en pacientes obesos mediante la terapia cognitivo-conductual administrada conjuntamente con la presoterapia. Métodos: Se realizó un estudio experimental con grupo control placebo. Se asignaron de forma aleatoria 40 pacientes a grupo de intervención (terapia cognitivo-conductual + presoterapia) y a grupo control (sesiones informativas). La dimensión de estudio fue la administración de un cuestionario...

  8. Evaluación de las terapias ecuestres en el tratamiento del trastorno de espectro autista y programación de intervención

    OpenAIRE

    Tabares Sánchez, Carmen

    2016-01-01

    Las Terapias Asistidas con Caballos están proliferando cada vez más como actividad terapéutica y representan una parte importante de la oferta de servicios en los centros ecuestres. Una de sus aplicaciones más frecuentes es la estimulación de personas con Trastorno de Espectro Autista (TEA). Sin embargo, hay escasez de estudios científicos que corroboren los efectos de esta técnica. El objetivo de esta investigación es verificar objetivamente si las Terapias Ecuestres conducen a una mej...

  9. Terapias cognitivo conductuales para el tratamiento de los trastornos de personalidad

    Directory of Open Access Journals (Sweden)

    Manuela Navarro-Leis

    2013-06-01

    Full Text Available Se han propuesto muchos tratamientos psicológicos para el abordaje de los trastornos de personalidad, realizándose gran variedad de estudios para comprobar la eficacia de los mismos. Y aunque hasta la fecha no se ha podido demostrar empíricamente que exista ningún tratamiento establecido como eficaz, si se ha demostrado que los tratamientos cognitivo conductuales y conductuales resultan probablemente eficaces para el tratamiento de estos trastornos, en concreto y de forma más específica para el Trastorno Límite de Personalidad. El objetivo de este trabajo es ofrecer una revisión a nivel teórico y empírico de los tres modelos de tratamiento considerados actualmente de mayor eficacia: Terapia Dialéctica Conductual, Terapia de Aceptación y Compromiso y Psicoterapia Analítico Funcional, incluidas dentro de las llamadas Terapias de conducta de tercera generación.

  10. Mycelial pellet formation by edible ascomycete filamentous fungi, Neurospora intermedia.

    Science.gov (United States)

    Nair, Ramkumar B; Lennartsson, Patrik R; Taherzadeh, Mohammad J

    2016-12-01

    Pellet formation of filamentous fungi in submerged culture is an imperative topic of fermentation research. In this study, we report for the first time the growth of filamentous ascomycete fungus, Neurospora intermedia in its mycelial pellet form. In submerged culture, the growth morphology of the fungus was successfully manipulated into growing as pellets by modifying various cultivation conditions. Factors such as pH (2.0-10.0), agitation rate (100-150 rpm), carbon source (glucose, arabinose, sucrose, and galactose), the presence of additive agents (glycerol and calcium chloride) and trace metals were investigated for their effect on the pellet formation. Of the various factors screened, uniform pellets were formed only at pH range 3.0-4.0, signifying it as the most influential factor for N. intermedia pellet formation. The average pellet size ranged from 2.38 ± 0.12 to 2.86 ± 0.38 mm. The pellet formation remained unaffected by the inoculum type used and its size showed an inverse correlation with the agitation rate of the culture. Efficient glucose utilization was observed with fungal pellets, as opposed to the freely suspended mycelium, proving its viability for fast-fermentation processes. Scale up of the pelletization process was also carried out in bench-scale airlift and bubble column reactors (4.5 L).

  11. Cuanta más psicología, mejor: eficacia para dejar de fumar. De la terapia cognitiva conductual intensiva y de los parches de nicotina combinados con terapia cognitiva conductual intensiva y menos intensiva

    Directory of Open Access Journals (Sweden)

    Ignacio Gabino Fernández Arias

    2014-01-01

    Full Text Available Para comparar la eficacia para dejar de fumar de la terapia cognitiva conductual intensiva (TCC/I, la TCC/I con parches de nicotina (TCC/I+PN y la terapia cognitiva conductual no intensiva con parches de nicotina (TCC/NI+PN se realizó un estudio experimental de medidas repetidas con 235 fumadores adultos asignados aleatoriamente a uno de esos tres tratamientos. Entre los pacientes que completaron el tratamiento (n= 152 la TCC/I+PN mostró tasas de abstinencia a los 6 meses y al año, mayores que la TCC/NI+PN, mientras que la TCC/I mostró tasas de abstinencia que no eran significativamente diferentes de las de los otros dos tratamientos. Estos resultados sugieren que los parches de nicotina se deberían utilizar siempre con una terapia cognitiva conductual lo más intensiva posible y que la utilización de esta última terapia en solitario debería gozar de mayor relevancia en las guías clínicas para dejar de fumar.

  12. Prevotella intermedia upregulates MMP-1 and MMP-8 expression in human periodontal ligament cells.

    Science.gov (United States)

    Guan, Su-Min; Shu, Lei; Fu, Shan-Min; Liu, Bin; Xu, Xiu-Li; Wu, Jun-Zheng

    2009-10-01

    Prevotella intermedia, a major periodontal pathogen, plays important roles in the initiation and development of periodontitis by stimulating the release of proinflammatory cytokines, proteinases and matrix metalloproteinases (MMPs). Our previous study demonstrated that P. intermedia induced MMP-9 expression in human periodontal ligament (hPDL) cells. In this study, we examined the effects of P. intermedia on other MMPs' expression. Semi-quantitative reverse transcriptase (RT)-PCR analysis revealed that P. intermedia ATCC 25611 supernatant increased MMP-1 and MMP-8 mRNA expression in a concentration- and time-dependent manner. Enzyme-linked immunosorbent assay and Western blot results confirmed the RT-PCR results at the protein level. Cyclooxygenase inhibitor indomethacin significantly attenuated the upregulatory effects of P. intermedia on MMP-1 and MMP-8 expression. Extracellular signal-related kinase inhibitor PD98059 and c-Jun N-terminal kinase inhibitor SP600125 considerably decreased the upregulated level of MMP-1, whereas p38 inhibitor SB203580 markedly inhibited MMP-8 expression, suggesting that prostaglandin E(2) and mitogen-activated protein kinase signaling pathways are involved in P. intermedia-induced MMP-1 and MMP-8 upregulation. Our results indicate that P. intermedia might contribute to periodontal connective tissue and bone matrix destruction through upregulating MMP production.

  13. Decontaminant solution on in vitro growth of Byrsonima intermedia seedlings

    Directory of Open Access Journals (Sweden)

    Luciano Coutinho Silva

    2015-04-01

    Full Text Available Byrsonima intermedia A. Juss. é uma planta medicinal e frutífera do Cerrado, cuja propagação convencional é difícil, devido à presença do endocarpo extremamente lignificado. Embora o hipoclorito de sódio (NaOCl seja amplamente utilizado para a desinfestação superficial, existem poucos relatos de seus efeitos sobre o crescimento do explante. Neste trabalho, o objetivo foi estudar o efeito de diferentes pHs e períodos de exposição de sementes de B. intermedia em uma solução de NaOCl. As sementes foram submetidas a diferentes tempos de exposição (1, 5 e 10 minutos numa solução de NaOCl com diferentes pHs (5, 7, 10 e 12 e, após o tratamento com hipoclorito de sódio, os embriões foram inoculados em meio WPM com a concentração de 50% de sais sem sacarose, ágar a 0,5% e pH 5,8 e, depois de 75 dias de cultivo, o crescimento das plântulas foi avaliado. A utilização de NaOCl é eficaz na desinfestação de sementes de B. intermedia, independente da variação do pH ou dos períodos de exposição. A porcentagem de plântulas normais, o comprimento da parte aérea e o número de folhas são positivamente afetados pela utilização de solução de NaOCl a pH de 8,5-8,9 ou pelo aumento do período de exposição, enquanto o número de raízes é afetado apenas pelo aumento do período de exposição na solução NaOCl

  14. Susceptibilities of Eikenella corrodens, Prevotella intermedia, and Prevotella nigrescens Clinical Isolates to Amoxicillin and Tetracycline

    Science.gov (United States)

    Luong, N.; Tsai, J.; Chen, C.

    2001-01-01

    The AB Biodisk Etest showed that 106 (100%) and 98 (92%) isolates of Eikenella corrodens were susceptible to amoxicillin and tetracycline, respectively. Twenty-three (68%) Prevotella intermedia isolates and 14 (67%) Prevotella nigrescens isolates were susceptible to amoxicillin. Seventy-nine percent of the P. intermedia isolates and 67% of the P. nigrescens isolates were susceptible to tetracycline. A higher percentage of β-lactamase-producing isolates of P. intermedia and P. nigrescens were identified with selective agar containing amoxicillin than with nonselective agar. PMID:11600394

  15. Efectos de las terapias ecuestres en personas con parálisis cerebral

    Directory of Open Access Journals (Sweden)

    Augusto Jiménez de la Fuente

    2017-12-01

    Full Text Available El objetivo del artículo es revisar la literatura científica existente a fin de comprobar los efectos de las terapias ecuestres en personas con parálisis cerebral. Se realizó una revisión bibliográfica en las bases de datos de Medline (PubMed, Dialnet, Scopus, Sportdiscus y Web of Science, estableciendo como criterios de inclusión para seleccionar los estudios clínicos por revisar que estos hubieran sido publicados entre 2001 y 2016, en castellano o inglés. Asimismo, se rechazaron aquellos en los que los sujetos recibieran una combinación de tratamientos farmacológicos. Se revisaron un total de 68 artículos, de los cuales 20 cumplieron los criterios de inclusión. Se analizaron la población objeto de estudio, el tipo de tratamiento, la temporalización y los resultados obtenidos. En general, se observan beneficios significativos en el uso de las terapias ecuestres como tratamiento para personas con parálisis cerebral.

  16. CULTURA DE SEGURANÇA DO PACIENTE EM TERAPIA INTENSIVA: RECOMENDAÇÕES DA ENFERMAGEM

    Directory of Open Access Journals (Sweden)

    Janeide Freitas de Mello

    2013-01-01

    Full Text Available Estudio cuantitativo, de tipo investigativo, transversal y comparativo que tuvo por objeto sistematizar las recomendaciones de enfermería sobre la seguridad del paciente en dos Unidades de Terapia Intensiva adulta de la Grande Florianópolis-SC, Brasil, en 2011. Resultó de la respuesta a una pregunta cualitativa aplicada con el Hospital Survey on Patient Safety Culture para 97 profesionales, con una tasa de respuesta del 93,8% correspondiente a 91 profesionales, y obtuvo 267 recomendaciones, clasificadas de acuerdo con las dimensiones del instrumento utilizado. Hubo un mayor número de recomendaciones para las dimensiones: aprendizaje organizacional y la mejora continua, con sugerencias envolviendo a la capacitación y al entrenamiento; personal en relación con la cuestión cuantitativa; y la percepción general de la seguridad, indicándose la mejoría de los procedimientos, los procesos y el apoyo de la gestión hospitalaria, dando énfasis a la mejoría de los recursos materiales y equipamientos. Destacadas por otros estudios, estas recomendaciones son esenciales para la promoción de la seguridad del paciente en las Unidades de Terapia Intensiva estudiadas.

  17. Approaches to management of beta-thalassemia intermedia

    Directory of Open Access Journals (Sweden)

    Joseph E. Maakaron

    2013-03-01

    Full Text Available Thalassemia intermedia is a genetically diverse group of diseases that is the result of an imbalance in the production of the alpha and beta chains with ensuing chronic hemolysis, ineffective erythropoiesis, and iron overload.Resulting complications include bone changes, hypercoagulability, and end-organ damage due to iron overload. This decade has witnessed major breakthroughs in the management of thalassemia. In this article, we examine these novelties in therapy including iron chelation therapy, stem cell transplant, and gene therapy.Iron chelation therapy has been revolutionized with the advent of deferasirox, a once-daily oral iron chelator, that has been shown to be safe and efficacious.Gene therapy was also at the core of this revolution with the discovery of novel gene elements and viral vectors allowing for better control and improved outcomes.

  18. Paraspinal extramedullary hematopoiesis in patients with thalassemia intermedia.

    Science.gov (United States)

    Haidar, Rachid; Mhaidli, Hani; Taher, Ali T

    2010-06-01

    Ineffective erythropoiesis in patients with thalassemia intermedia drives extramedullary hematopoietic tumor formation in several parts of the body. Paraspinal involvement has received increasing attention due to the associated morbidity secondary to spinal cord compression. Although the history and physical examination may help narrow the differential diagnosis, radiographic imaging remains essential to confirm the existence of hematopoietic tissue. Characteristic appearance has been observed mainly on magnetic resonance imaging. Several treatment options have been described, including transfusion therapy, laminectomy, radiotherapy, and the use of fetal hemoglobin inducing agents that decrease the hematopoietic drive. However, the ideal management scheme remains controversial. Until large prospective trials evaluate the efficacy and safety of the available treatment options, both in single and in combination therapy, an individualized approach should be entertained.

  19. Myopathy in horses with pituitary pars intermedia dysfunction (Cushing's disease).

    Science.gov (United States)

    Aleman, M; Watson, J L; Williams, D C; LeCouteur, R A; Nieto, J E; Shelton, G D

    2006-11-01

    Fifteen horses with pituitary pars intermedia dysfunction were studied. The horses were of various breeds and between 15 and 28 years of age. Control horses matched for breed and age were studied for comparison. Evaluations included complete blood cell count and serum biochemical analysis, electromyography, and gluteus medius muscle biopsies for histochemical, morphometric, and ultrastructural analysis. No differences were found between groups of horses on routine laboratory analysis or electromyography. We demonstrated that muscle wasting in diseased horses was the result of atrophy of types 2A and 2B muscle fibers and loss of type 2B myofibers. Mild non-specific non-inflammatory myopathic alterations such as myofiber size variation, internal nuclei, perimysial, endomysial and sarcoplasmic fat accumulation were observed. At the ultrastructural level, subsarcolemmal mitochondrial accumulation and increased lipid droplets were evident. Similar to other species, this study confirmed atrophy of type 2 fibers as the cause of muscle mass loss in horses with Cushing's disease.

  20. Lipoproteína a, aterosclerosis y terapia hormonal de reemplazo Lipoprotein a, atherosclerosis and replacement hormone therapy

    Directory of Open Access Journals (Sweden)

    Miguel Lugones Botell

    2005-08-01

    Full Text Available Se realizó una revisión sobre la lipoproteína plasmática, Lp(a, cuyo papel fisiológico es poco conocido. Se ha descrito una asociación entre las concentraciones aumentadas de Lp(a y el proceso aterosclerótico. Además, su exceso podría inducir una disminución de la actividad fibrinolítica y, por tanto, favorecer la trombosis. También analizamos la terapia hormonal de reemplazo. En relación con los efectos positivos, mejora los síntomas climatéricos y previene la osteoporosis, aunque entre los efectos adversos en las mujeres que la siguen, se ha descrito un ligero aumento del riesgo del tromboembolismo venoso, y más recientemente, en estudios realizados en EE.UU. en los años 2002 y 2004, en el ya conocido estudio (Women´s Health Initiative Study, se reportó mayor incidencia de eventos cardiovasculares para la terapia combinada con estrógenos conjugados equinos y medroxiprogesterona, y de stroke para la terapia estrogénica. Estos estudios pusieron en su lugar los efectos de esta terapia, que no es totalmente inocua. Se precisan estudios más amplios para definir el papel de la terapia hormonal de reemplazo y otras medidas terapéuticas sobre el sistema hemostático, el metabolismo lipídico y la enfermedad cardiovascular.A review of plasmatic lipoprotein, Lp(a, whose physiological role is little known, was made. An association between the augmented concentrations of Lp(a and the atherosclerotic proccess has been described. Besides, its excess may lead to a reduction of the fibrinolytic activity and, therefore, favor thrombosis. The replacement hormone therapy was also analyzed. In relation to its positive effects, it improves the climacteric symptoms and prevents osteoporosis. Among its adverse effects, it has been observed a mild increase of the risk for venous thromboembolism and, more recently, in the aleady known Women's Health Initiative Study, it was reported a higher incidence of cardiovascular events for the combined

  1. Splenectomy for people with thalassaemia major or intermedia.

    Science.gov (United States)

    Easow Mathew, Manu; Sharma, Akshay; Aravindakshan, Rajeev

    2016-06-14

    Thalassaemia is a genetic disease of the haemoglobin protein in red blood cells. It is classified into thalassaemia minor, intermedia and major, depending on the severity of the disease and the genetic defect. Thalassaemia major and intermedia require frequent blood transfusions to compensate for the lack of well-functioning red blood cells, although this need is significantly less in thalassaemia intermedia.Damaged or defective red blood cells are normally eliminated in the spleen. In people with thalassaemia there is a large quantity of defective red blood cells which results in an enlarged hyperfunctioning spleen (splenomegaly). Removal of the spleen may thus prolong red blood cell survival by reducing the amount of red blood cells removed from circulation and may ultimately result in the reduced need for blood transfusions. To assess the efficacy and safety of splenectomy in people with beta-thalassaemia major or intermedia. We searched the Cochrane Cystic Fibrosis and Genetic Disorders Review Group's Haemoglobinopathies Trials Register, compiled from searches of electronic databases and the handsearching of journals and conference abstract books. We also searched the reference lists of relevant articles and reviews.Date of the most recent search: 25 April 2016. We included randomised controlled studies and quasi-randomised controlled studies of people of any age with thalassaemia major or intermedia, evaluating splenectomy in comparison to conservative treatment (transfusion therapy and iron chelation) or other forms of splenectomy compared to each other (laparoscopic, open, radio-frequency). Two authors independently selected and extracted data from the single included study using a customised data extraction form and assessed the risk of bias. One study, including 28 participants was included in the review; the results were described, primarily, in a narrative manner. The study assessed the feasibility of splenectomy using laparoscopy in comparison to open

  2. Terapia gênica Gene therapy

    Directory of Open Access Journals (Sweden)

    Nance Beyer Nardi

    2002-01-01

    Full Text Available Terapia gênica é um procedimento médico que envolve a modificação genética de células como forma de tratar doenças. Os genes influenciam praticamente todas as doenças humanas, seja pela codificação de proteínas anormais diretamente responsáveis pela doença, seja por determinar suscetibilidade a agentes ambientais que a induzem. A terapia gênica é ainda experimental, e está sendo estudada em protocolos clínicos para diferentes tipos de doenças. O desenvolvimento de métodos seguros e eficientes de transferência gênica para células humanas é um dos pontos mais importantes na terapia gênica. Apesar do grande esforço dirigido na última década para o aperfeiçoamento dos protocolos de terapia gênica humana, e dos avanços importantes na pesquisa básica, as aplicações terapêuticas da tecnologia de transferência gênica continuam ainda em grande parte teóricas. O potencial da terapia gênica é muito grande, devendo ainda causar grande impacto em todos os aspectos da medicina.Gene therapy is a medical intervention that involves modifying the genetic material of living cells to fight disease. Genes influence virtually every human disease, either by encoding for abnormal proteins, which are directly responsible for the disease, or by causing a susceptibility to environmental agents which induce it. Gene therapy is still experimental, and is being studied in clinical trials for many different types of diseases. The development of safe and effective methods of implanting normal genes into the human cell is one of the most important technical issues in gene therapy. Although much effort has been directed in the last decade toward improvement of protocols in human gene therapy, and in spite of many considerable achievements in basic research, the therapeutic applications of gene transfer technology still remain mostly theoretical. The potential for gene therapy is huge and likely to impact on all aspects of medicine.

  3. Traducción al castellano de la Terapia de Activación Conductual para la depresión y adaptación a formato grupal. Datos preliminares de su aplicación en una muestra de pacientes deprimidos

    OpenAIRE

    Sánchez González, David

    2011-01-01

    La activación conductual se ha mostrado igual de eficaz que otras terapias bien establecidas para el tratamiento de la depresión, sin embargo todavía faltan por implementar versiones breves de la misma al formato grupal. En el presente estudio se evaluó si la Terapia de Activación Conductual para la Depresión adaptada a formato grupal produce una reducción de la sintomatología depresiva en el trastorno depresivo mayor. La idea central de la terapia es que los pacientes incrementen el número d...

  4. In vitro Resistance Testing of Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia to Triclosan.

    Science.gov (United States)

    Farsi, Deema; Tanner, Anne

    2016-04-01

    To determine the sensitivity of Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia to triclosan, and determine if these bacteria develop resistance to triclosan upon prolonged exposure. Susceptibility to triclosan was tested against three periodontal pathogens P. gingivalis, P. intermedia, and T. forsythia. Escherichia coli strains sensitive and resistant to triclosan were used as biological controls to confirm the efficacy of triclosan in the assays. Agar plates were prepared locally with vitamin K and hemin-supplemented medium. Porphyromonas gingivalis and P. intermedia did not grow on plates containing ≥ 2 μg/ml triclosan, while T. forsythia did not grow on ≥ 1.66 μg/ml. Colonies of P. intermedia resistant to triclosan developed after prolonged incubation at 2 μg/ml, but this resistance disappeared during subculture in the absence of triclosan. No significant resistance to triclosan was detected for these species. Dental products containing triclosan can be beneficial in controlling periodontal disease.

  5. Development of Lutzomyia intermedia and Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae larvae in different diets

    Directory of Open Access Journals (Sweden)

    WERMELINGER E. D.

    2001-01-01

    Full Text Available The objective of this research was to evaluate, in laboratory, the development of Lutzomyia intermedia and Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae larvae, vectors of leishmaniasis in Brazil, in the following diets: industrialized food for rabbits, dogs, hamsters and aquarium fishes, besides liver powder, cooked lettuce, wheat germ, beer yeast, oat, wheat bran and a diet denominated aged food. Except wheat bran for L. intermedia, all diets provided adequate development for both species, which showed that any of them can be used in laboratory insectaries for these insects. L. intermedia showed better development with most nutritious diets and both species presented better development with aged food. Fungi as an additional nutrient source for L. intermedia and L. longipalpis is suggested.

  6. Prevotella intermedia infection causing acute and complicated aortitis—A case report

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    C. Boersma

    2017-01-01

    CONCLUSION: Prevotella intermedia is a rare causative agent for aortitis. Acute aortitis is a challenging clinical entity which should be managed in an equipped medical center by an experienced multidisciplinary team.

  7. ELISA for the detection of venom antigens in experimental and clinical envenoming by Loxosceles intermedia spiders.

    Science.gov (United States)

    Chávez-Olórtegui, C; Zanetti, V C; Ferreira, A P; Minozzo, J C; Mangili, O C; Gubert, I C

    1998-04-01

    Enzyme linked immunosorbent assays (ELISA) were developed to detect antigens from Loxosceles intermedia spider venom. Hyperimmune horse anti-Loxosceles intermedia IgGs were prepared by immunoaffinity chromatography and used to set up a sandwich-type ELISA. The specificity of the assay was demonstrated by its capacity to correctly discriminate the circulating antigens in mice that were experimentally inoculated with L. intermedia venom from those inoculated with L. gaucho, L. laeta, and Phoneutria nigriventer spider venoms, Tityus serrulatus scorpion venom and Bothrops jararaca, Crotalus durissus terrificus, Lachesis muta muta and Micrurus frontalis snake venoms. Measurable absorbance signals were obtained with 0.8 ng of venom per assay. The ELISA also detected antigens in the sera of patients envenomed by L. intermedia. Therefore, after standardization for clinical use this ELISA may be a valuable tool for clinicians and epidemiologists.

  8. LA TERAPIA DE ACEPTACIÓN Y COMPROMISO PARA LOS TRASTORNOS POR ABUSO Y DEPENDENCIA DE SUSTANCIAS PSICOACTIVAS: UNA REVISIÓN DESCRIPTIVA

    Directory of Open Access Journals (Sweden)

    Laura Fernanda Perea-Gil

    2017-01-01

    Full Text Available El abuso y la dependencia de sustancias psicoactivas es una de las problemáticas asociadas a mayor deterioro en las diferentes áreas de ajuste de las personas que los presentan. Adicional a lo anterior, dentro de los tratamientos centrados en esta problemática, se presentan altos porcentajes de deserción y recaídas. Sobre esta base, resulta pertinente encontrar tratamientos psicológicos que puedan ser eficaces en el abordaje de dicha problemática. Con lo anterior, en las últimas décadas han surgido las denominadas Terapias de Tercera Generación, entre ellas, la Terapia de Aceptación y Compromiso que ha demostrado efectividad en el abordaje de las drogodependencias. Teniendo en cuenta esto, el objetivo del artículo es presentar una revisión de los estudios empíricos en adicciones basados en la Terapia de Aceptación y Compromiso, a partir de la búsqueda y el análisis de la literatura científica. De acuerdo con los resultados, se concluye que la Terapia de Aceptación y Compromiso es prometedora para el abordaje de las adicciones, sin embargo, se hace necesario realizar un mayor número de estudios, con el fin de ampliar su cuerpo empírico.

  9. Detection and comparison of specific hemin binding by Porphyromonas gingivalis and Prevotella intermedia.

    OpenAIRE

    Tompkins, G R; Wood, D P; Birchmeier, K R

    1997-01-01

    A radioligand assay was designed to detect and compare specific hemin binding by the periodontal anaerobic black-pigmenting bacteria (BPB) Porphyromonas gingivalis and Prevotella intermedia. The assay included physiological concentrations of the hemin-binding protein rabbit serum albumin (RSA) to prevent self-aggregation and nonspecific interaction of hemin with cellular components. Under these conditions, heme-starved P. intermedia cells (two strains) expressed a single binding site species ...

  10. Terapia psicológica para la esquizofrenia: Una realidad

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    Jennifer Del Toro Granados

    2013-10-01

    Full Text Available Resumen La esquizofrenia es entendida como un cuadro psiquiátrico, crónico y altamente discapacitante; en la cual, a pesar de las variaciones de la evolución en cada paciente, se observan los siguientes síntomas: ideas delirantes, alucinaciones, comportamiento desorganizado (síntomas positivos y aplanamiento afectivo (síntomas negativos. A finales de los años 70 se produce un cambio en la concepción etiológica de los trastornos psicóticos que comienzan a explicarse desde una perspectiva multicausal, es decir, como predeterminados e influidos por condiciones biopsicosociales. Uno de los más completos paquetes de intervención es la Terapia Psicológica Integral para la esquizofrenia (IPT. Los autores de este protocolo terapéutico parten del supuesto que los pacientes que reúnen criterios para esquizofrenia muestran déficits en varios niveles funcionales de organización de la conducta (nivel atencional-perceptivo, nivel cognitivo, nivel micro-social y nivel macro-social. En el presente análisis, se describen los hallazgos en términos de eficacia de la terapia psicológica integral para la esquizofrenia combinada con farmacoterapia; los distintos estudios reseñados indican que la IPT muestra superioridad terapéutica frente a otro tipo de intervenciones. (DUAZARY 2011, 93 - 98 AbstractSchizophrenia is defined as a table psychiatric, chronic and highly disabling, in the which, despite changes in the evolution of each patient, observe the following symptoms: ideas delusions, hallucinations, disorganized behavior (positive symptoms and blunted affect (symptoms negative. In the late 70’s is a change in the etiological concept of psychotic disorders starting to explain from a causes, ie as default and influenced by biopsychosocial conditions. One of the most complete packages of intervention is the therapy Comprehensive psychological schizophrenia (IPT. The authors of this therapeutic format asume Patients who meet criteria for

  11. El rol de las proteínas tirosina quinasas ZAP-70 y Syk en la activación de las células T: Estudios preclínicos de terapia génica para la deficiencia en ZAP-70

    OpenAIRE

    Steinberg, Marcos Wenceslao

    2003-01-01

    Las proteínas ZAP-70 y Syk componen la familia Syk de tirosina quinasas. Estas dos proteínas presentan secuencias de amino ácidos y estructuras similares, sin embargo ZAP-70 y Syk son reguladas diferentemente en términos de expresión y probablemente de función. En los linfocitos T, estas quinasas intervienen en la diferenciación y la activación celular mediada por el receptor de células T (TCR). En este trabajo hemos sido capaces de realizar un estudio comparativo del rol de ZAP-70 y de Syk e...

  12. Efectos de una terapia combinada de ejercicio terapéutico basado en el método pilates y movilización neuromeníngea en pacientes con dolor lumbar crónico inespecífico. Estudio de casos.

    OpenAIRE

    González Sánchez, Andrea

    2014-01-01

    Trabajo Fin de Grado (TFG) Objetivo: Conocer los efectos de un programa de tratamiento que combina el ejercicio terapéutico basado en el método Pilates y la Movilización Neuromeníngea en pacientes con dolor lumbar crónico inespecífico irradiado a miembros inferiores. Diseño: Estudio de casos. Participantes: Muestra de 7 pacientes, todas ellas mujeres de entre 39 y 61 años de edad, que presentan dolor lumbar crónico inespecífico irradiado a miembros inferiores. Intervención: Se realizó un p...

  13. Prevotella intermedia Stimulates Expansion of Vβ-Specific CD4+ T Cells

    Science.gov (United States)

    Leung, K.-P.; Torres, Barbara A.

    2000-01-01

    Recent evidence suggests that certain periodontal pathogens preferentially stimulate T cells expressing specific variable regions on the β chain (Vβ) of the T-cell receptor, which may indicate the presence of a superantigen. Superantigens are microbial proteins that activate large numbers of CD4+ T cells in a Vβ-specific manner. The purpose of this study was to determine whether Prevotella intermedia, a putative periodontal pathogen, activates populations of specific Vβ on CD4+ T cells. Among the bacterial strains tested, P. intermedia strain 17, a clinical isolate, induced the strongest proliferative response in peripheral blood mononuclear cells. Antibodies raised against whole cells of this organism blocked the proliferative activity. P. intermedia-induced proliferation was T-cell specific and required the presence of antigen-presenting cells. Flow cytometric analysis showed that CD4+ T-cell subsets expressing Vβ8, Vβ12, and Vβ17 expanded in response to P. intermedia strain 17. The ability of P. intermedia to stimulate CD4+-T-cell proliferation was further supported by the production profiles of key T-cell cytokines, gamma interferon and interleukin-2. The data collectively suggest that certain strains of P. intermedia can activate Vβ-specific T cells in a manner similar to that of other known microbial superantigens. PMID:10948175

  14. Protein Profile of Prevotella intermedia and Porphyromonas gingivalis Cell Isolated from Chronic Periodontitis Patient

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    Jessica Setyadarma Loviamanda

    2013-07-01

    Full Text Available Prevotella intermedia and Porphyromonas gingivalis are two of the specific microorganisms that frequently occurs in chronic periodontitis. As protein plays an important role in bacteria's life and its virulence factor, we tried to explore variation in P. intermedia and P. gingivalis cell protein profile which isolated from different periodontl pocket depth of chronic periodontitis patient. P. intermedia and P. gingivalis were identified using Polymerase Chain Reaction (PCR technique, and its protein profile were evaluated using Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE. SDS-PAGE result showed that there were significant difference between protein profile of P. intermedia cell which isolated from slhallow and deep pocket with dominant proteins of molecular weight 200 kDa, 110 kDa, 40 kDa, and 25 kDa, however, relationship between pocket depth and P. intermedia cell protein pofile could not be concluded because unrepresentative number of P. intermedia colonies. In the other hand the variation in P. gingivalis cell protein profile was not influenced by pocket depth with dominant protein called hemin binding protein (HbBp.DOI: 10.14693/jdi.v17i1.110

  15. Estudo fenomenológico sobre a vivência da morte em uma unidade de terapia intensiva neonatal Estudio fenomenológico sobre la vivencia de la muerte en la unidad de terapía intensiva neonatal Phenomenologic study about experiences when living the death in the neonatal critical care unit

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    Laureana Cartaxo Salgado Pereira Silva

    2010-10-01

    Full Text Available Esta investigação teve como objetivos: descrever a vivência de cuidado de profissionais de enfermagem de unidade de terapia intensiva neonatal diante da morte e compreender os sentimentos diante da morte do recém-nascido. Pesquisa qualitativa com abordagem fenomenológica, tendo como pergunta norteadora: Como você se sente diante da morte do recém-nascido na UTI onde trabalha? Participaram da entrevista 12 enfermeiros e técnicos de enfermagem da UTI. Emergiram sentimentos como culpa, fracasso e negação. Compreendendo o fenômeno estudado, afirmamos que a morte do recém-nascido no espaço da UTI é uma vivência de sentimentos conflituosos, por vezes dolorosos para os profissionais de enfermagem.Esta investigación tuvo como objetivo describir la experiencia de la atención de las enfermeras de la unidad neonatal de cuidados intensivos antes de la muerte y comprender los sentimientos antes de la muerte del recién nacido. Investigación cualitativa con abordaje fenomenológico, con la pregunta orientadora: ¿Cómo te sientes acerca de la muerte de los recién nacidos en la UCI donde usted trabaja? Asistió a la entrevista 12 enfermeras y la enfermería en la UCI. Surgido como sentimientos de culpa, el fracaso y la negación. Entender el fenómeno estudiado, afirmamos que la muerte del recién nacido dentro de la UCI es una experiencia de sentimientos contradictorios, a veces dolorosas para las enfermeras.This research aimed at describing the care experiences of neonatal critical care nurses when facing the death and to understand their fellings before the death of the newborn. Qualitative research with a phenomenological approach, with the guiding question: How do you feel about the death of the newborn ICU where you work? Attended the interview 12 nurses and ICU nursing. Emerging feelings such as guilt, failure and denial. Understanding the phenomenon being studied, we affirm that the death of the newborn within the ICU is an

  16. Tratamiento del hombro doloroso mediante terapia manual

    OpenAIRE

    Gabucio López, Pedro

    2008-01-01

    Introducción: Las disfunciones de hombro son un problema de salud común en las sociedades occidentales. Algunos protocolos de tratamiento han sido desarrollados mediante ensayos clínicos con pacientes que presentan dolor de hombro. Sin embargo, no hay evidencias que sustenten que un protocolo es mejor que otros. El principal objetivo de este trabajo es presentar un caso clínico en el que mediante terapia manual y la prescripción de ejercicios físicos se consigue la resolución del ...

  17. Los ritmos como terapia para la impulsividad

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    Mónica Triviño

    2012-10-01

    Full Text Available Investigaciones recientes muestran que el uso de patrones rítmicos facilita la respuesta óptima en el tiempo, por lo que el entrenamiento mediante ritmos podría proponerse como terapia novedosa ante problemas como la impulsividad. Esto podría beneficiar a pacientes con daño prefrontal o personas con trastorno por déficit de atención e hiperactividad (TDAH, que suelen mostrar conductas impulsivas, así como dificultad para estimar el paso del tiempo.

  18. Terapia Ocupacional Pediátrica

    OpenAIRE

    Pérez Fernández, Germán

    2016-01-01

    El artículo muestra la naturaleza y las actividades propias de la Terapia Ocupacional, y especifica sus posibilidades para promover el desarrollo global de los niños con Síndrome de Down, de modo que alcancen un nivel óptimo de funcionamiento en el hogar, la escuela y el juego o, lo que es lo mismo, logrando así un nivel de autonomía e independencia. Describe el procesamiento e integración sensorial, es decir, su capacidad para organizar e integrar los estímulos sensoriales, aplicándolos a la...

  19. Terapia cognitivo-conductual y psicosis

    OpenAIRE

    Cuevas Yust, Carlos; Perona Garcelán, Salvador Félix

    2002-01-01

    En una patología, la psicosis, tradicionalmente reservada al tratamiento psiquiátrico, resulta especialmente interesante y prometedora la aparición de las intervenciones psicológicas. De hecho, la terapia cognitivo-conductual (TCC) está mostrando ser una aproximación efectiva en el tratamiento de síntomas psicóticos positivos resistentes a la medicación neuroléptica, existiendo incluso alguna evidencia de que podría contribuir a la reducción de las recaídas. En el presente trabajo describimos...

  20. Primer aislamiento en Argentina de Staphylococcus aureus resistente a la meticilina adquirido en la comunidad con sensibilidad intermedia a la vancomicina y no sensibilidad a la daptomicina

    Directory of Open Access Journals (Sweden)

    Laura Errecalde

    Full Text Available Describimos el primer caso en Argentina de Staphylococcus aureus resistente a la meticilina adquirido en la comunidad con sensibilidad intermedia a la vancomicina y no sensibilidad a la daptomicina. Caso clínico: Paciente masculino, con antecedente de insuficiencia renal crónica en hemodiálisis y osteosíntesis de cadera debido a una fractura. Se internó por síndrome febril persistente luego del desplazamiento de la prótesis por un traumatismo. Se aisló de hemocultivos S. aureus resistente a la meticilina adquirido en la comunidad. Durante el tratamiento con vancomicina y daptomicina, se observó un aumento gradual de la CIM de vancomicina de 1 µg/ml (VSSA a 2 µg/ml (h-VISA y a 4 µg/ml (VISA, y la emergencia de no sensibilidad a daptomicina (CIM = 4 µg/ml. Al suspender la vancomicina y la daptomicina, la cepa revirtió al fenotipo de sensibilidad a ambas drogas. Es mandatorio evaluar por CIM la sensibilidad a vancomicina y a daptomicina intratratamiento cuando estas drogas se usan como terapia.

  1. Evaluación del impacto clínico y la seguridad de una unidad de dolor torácico en pacientes con probabilidad baja e intermedia de síndrome coronario agudo

    OpenAIRE

    Dagnovar Aristizábal O.; Cristina Montoya M.; Ángela M. Valencia C.; Juan M. Jaime T.; Jaime A. Gallo V.

    2015-01-01

    Antecedentes: Aunque las unidades de dolor torácico (UDT) permiten la estratificación y atención apropiada de los pacientes con dolor de pecho, en Colombia el desarrollo e implementación sistemática y estandarizada de este proceso de atención es escaso. Objetivo: Evaluar el impacto clínico y la seguridad del proceso de atención de una UDT en pacientes con probabilidad baja e intermedia de síndrome coronario agudo en Medellín, Colombia. Métodos: Estudio descriptivo que incluyó 277 pacien...

  2. Análisis de la eficacia de las acciones de juego del voleibol masculino y femenino de alto rendimiento en la fase inicial, intermedia y final de los sets

    OpenAIRE

    Batista Sucupira, Giuliano

    2014-01-01

    Tesis descargada desde TESEO En esta investigación, se ha presentado el análisis de la eficacia de todas las acciones de juego del voleibol, comparando equipos masculinos y femeninos de alto nivel en las fases inicial (0-8 puntos), intermedia (9-16 puntos) y final (17 hasta fin del set) de cada uno de los sets. Igualmente, se ha realizado el estudio de las correlaciones de las acciones de juego y las comparaciones entre los equipos ganadores y perdedores de los sets para ambos géneros. La ...

  3. Lamellar pathology in horses with pituitary pars intermedia dysfunction.

    Science.gov (United States)

    Karikoski, N P; Patterson-Kane, J C; Singer, E R; McFarlane, D; McGowan, C M

    2016-07-01

    Hoof lamellar pathology in horses with pituitary pars intermedia dysfunction (PPID) has not been described previously. To describe the histomorphometry and pathological lesions in hoof lamellar tissue of animals that had PPID with or without concurrent laminitis, with reference to age-matched controls. We hypothesised that lamellar lesions consistent with laminitis would be associated with PPID, even in animals without current or historical laminitis. Prospective case-control study. Mid-dorsal hoof histological sections were obtained post mortem from the forelimbs of 16 PPID-affected animals either with (n = 6) or without laminitis (n = 10) and 10 age- and breed-matched controls. Sections were examined by a blinded veterinary pathologist. The length and width of 10 primary epidermal lamellae were measured using image analysis software. The morphology and pathology of primary and secondary epidermal lamellae were then typed or graded in axial, middle and abaxial regions. Fasting serum insulin, plasma adrenocorticotropin and blood glucose concentration were measured from blood samples taken prior to euthanasia. All animals with PPID and laminitis had fasting hyperinsulinaemia (median 74.1 miu/l, interquartile range 49.9-349.5 miu/l) whereas PPID animals without laminitis had serum insulin concentrations below the upper limit of the reference range (morphological alteration and pathology consistent with laminitis. © 2015 EVJ Ltd.

  4. Células madre y terapia celular

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    Elso Manuel Cruz Cruz

    2015-03-01

    Full Text Available Una célula madre (CM o célula troncal es aquella capaz de dividirse indefinidamente y diferenciarse a distintos tipos de células especializadas, no sólo morfológica sino también funcionalmente. Las células madre pueden clasificarse atendiendo a su origen en células madre adultas (CMA y células madre embrionarias (CME. Por sus características, las CM tienen potencialidades para diversos usos en la práctica clínica. La primera es como vehículo terapéutico de genes, en el caso de enfermedades monogénicas o incluso como vehículo de terapias antitumorales o antiangiogénicas. La segunda y principal aplicación es, aprovechando su potencial de diferenciación, en la regeneración de tejidos destruidos o dañados, como terapia de reemplazo celular o medicina regenerativa. En esta área se están desarrollando trabajos de investigación donde se busca reemplazar células dañadas por otras que restituyan la función normal de los tejidos u órganos; en enfermedades como: diabetes mellitus, enfermedades cardiovasculares, enfermedad de Parkinson y enfermedades de células sanguíneas. Actualmente existen muchas investigaciones enfocadas a descubrir células progenitoras que sirvan como banco de células para usos terapéuticos. Se reporta el empleo de varias estrategias, las que incluyen: terapias celulares derivadas de células autólogas; terapias celulares derivadas de líneas celulares establecidas desde una variedad de CM que incluyen MO, cordón umbilical, CME, así como células de tejidos y órganos de animales genéticamente modificados. Las principales aplicaciones se realizan con CM de la médula ósea (MO, ya que esta es un recurso celular propio, accesible y abundante para la terapia celular. (1 En la MO existe un grupo heterogéneo de CMA; además de las hematopoyéticas (CMH están las: endoteliales, mesenquimales (CMM, de la población lateral, progenitoras adultas multipotentes, ovales y también células madre muy peque

  5. In Vitro Effects of Polyphosphate against Prevotella intermedia in Planktonic Phase and Biofilm.

    Science.gov (United States)

    Jang, Eun-Young; Kim, Minjung; Noh, Mi Hee; Moon, Ji-Hoi; Lee, Jin-Yong

    2016-02-01

    Polyphosphate (polyP) has gained a wide interest in the food industry due to its potential as a decontaminating agent. In this study, we examined the effect of sodium tripolyphosphate (polyP3; Na5P3O10) against planktonic and biofilm cells of Prevotella intermedia, a major oral pathogen. The MIC of polyP3 against P. intermedia ATCC 49046 determined by agar dilution method was 0.075%, while 0.05% polyP3 was bactericidal against P. intermedia in time-kill analysis performed using liquid medium. A crystal violet binding assay for the assessment of biofilm formation by P. intermedia showed that sub-MICs of polyP3 significantly decreased biofilm formation. Under the scanning electron microscope, decreased numbers of P. intermedia cells forming the biofilms were observed when the bacterial cells were incubated with 0.025% or higher concentrations of polyP3. Assessment of biofilm viability with LIVE/DEAD staining and viable cell count methods showed that 0.05% or higher concentrations of polyP3 significantly decreased the viability of the preformed biofilms in a concentration-dependent manner. The zone sizes of alpha-hemolysis formed on horse blood agar produced by P. intermedia were decreased in the presence of polyP3. The expression of the genes encoding hemolysins and the genes of the hemin uptake (hmu) locus was downregulated by polyP3. Collectively, our results show that polyP is an effective antimicrobial agent against P. intermedia in biofilms as well as planktonic phase, interfering with the process of hemin acquisition by the bacterium. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  6. Incidence of Prevotella intermedia and Prevotella nigrescens Carriage among Family Members with Subclinical Periodontal Disease

    Science.gov (United States)

    Fukui, Katsuhito; Kato, Naoki; Kato, Haru; Watanabe, Kunitomo; Tatematsu, Norichika

    1999-01-01

    We established a typing system for Prevotella intermedia and Prevotella nigrescens using the combination of PCR ribotyping and arbitrarily primed PCR (AP-PCR) fingerprinting and applied this system to the study of intrafamilial incidence of these species in the oral cavity. PCR ribotyping followed by subtyping by AP-PCR fingerprinting was applied to each type strain of P. intermedia and P. nigrescens and 54 isolates (32 isolates of P. intermedia and 24 isolates of P. nigrescens) from extraoral infections, resulting in an excellent discriminatory power (discrimination index, 0.99) for both species. A total of 18 subjects from six families, with the subjects from each family comprising the mother, the father, and a child who had subclinical early-stage to moderate adult periodontitis or simple gingivitis and who carried P. intermedia or P. nigrescens, or both, were enrolled in the study of intrafamilial carriage. When 20 colonies per specimen of subgingival plaque, if available, were picked from primary culture, 115 P. intermedia and 178 P. nigrescens isolates were recovered from the 18 subjects. Among the subjects studied, family members shared the same subtype strain(s) but non-family members did not. Multiple subtypes were found in 8 (57%) of the 14 P. nigrescens-positive subjects but in only 3 (27%) of the 11 P. intermedia-positive subjects; the difference was, however, not statistically significant (P = 0.14). These results suggest that the combination of PCR ribotyping and AP-PCR fingerprinting is well suited for the epidemiological study of P. intermedia and P. nigrescens and that each family seems to carry a distinct subtype(s) of these species. PMID:10488167

  7. Terapia trombolítica en pacientes con infarto agudo de miocardio en Cienfuegos

    Directory of Open Access Journals (Sweden)

    Lázaro Abilio Hernández Rodríguez

    2014-03-01

    Full Text Available Fundamento: el tratamiento trombolítico practicado a menos de dos horas, tras el inicio del infarto tiene resultados similares a la reperfusión mecánica. Objetivo: describir aspectos relevantes de la terapia trombolítica en pacientes con infarto agudo de miocardio con elevación del segmento ST en Cienfuegos. Métodos: estudio descriptivo, prospectivo, en 225 pacientes egresados (vivos y fallecidos desde el 1o de abril de 2012 al 31 marzo de 2013, de la Unidad de Cuidados Integrales del Corazón y la Unidad de Cuidados Intensivos Polivalentes, del Hospital Dr. Gustavo Aldereguía Lima. Las variables estudiadas se obtuvieron de la revisión de las historias clínicas e incluyeron: variables demográficas y clínicas, tiempos de demora, sitio, motivo de exclusión y nivel de prioridad para la trombolisis. Resultados: la media de edad superó los 60 años. Predominaron los hombres, hipertensos y de alto riesgo según la escala GRACE. El 29,7 % de los pacientes no recibió terapia trombolítica, debido mayormente al factor tiempo y la mayoría (82,3 % se trató en el Hospital. Las medianas de los tiempos de demora: síntomas-puerta, puerta-droga y síntomas-droga fueron 90, 35 y 140 minutos respectivamente. Conclusiones: la trombolisis extrahospitalaria acorta el tiempo total de isquemia, pero pocos pacientes se beneficiaron de ella. Los retrasos para la terapia trombolítica en Cienfuegos continúan por encima de los estándares requeridos.

  8. "Soy como un aspirador" : Medicina popular, Espiritualidad y New Age en las terapias de un curandero catalán

    OpenAIRE

    Riccò, Isabella

    2016-01-01

    El artículo presenta un estudio de caso de un “curandero” catalán, una figura eclética e interesante. Josep práctica distintos tipos de sanaciones: soluciona los males espirituales a través del trance, tira las cartas, cura la culebrilla (herpes zoster) con un cataplasma, distiende los nervios mediante una vela y saca la mala energía de los dedos. Sus terapias son muy heterogéneas y se han ido desarrollando durante los últimos 40 años. El objetivo del texto es acercarse al panorama de la medi...

  9. Estudio EPIFARM

    Directory of Open Access Journals (Sweden)

    Martín Morales A

    2010-12-01

    Full Text Available INTRODUCCIÓN Los farmacéuticos comunitarios pueden ser un importante primer punto de contacto con los pacientes con disfunción eréctil (DE, pero hasta la fecha no hay ningún estudio sobre las características de los hombres que acuden a un farmacéutico solicitando consejo o tratamiento para la DE. OBJETIVO Caracterizar los perfiles de los hombres que solicitan tratamiento para la DE en la farmacia, con o sin receta de inhibidores de la fosfodiesterasa tipo 5 (iPDE5. MÉTODOS Entre septiembre y noviembre de 2008 se realizó un estudio observacional, transversal y multicéntrico en farmacias comunitarias de España. De aquellos hombres que solicitaban consejo o tratamiento para la DE, cada investigador reclutó un paciente que tenía receta médica de iPDE5 y otro que acudía sin receta médica. Los farmacéuticos del estudio completaron un cuestionario de datos demográficos, clínicos y conductuales del paciente, incluido el Cuestionario de salud sexual para varones (Sexual Health Inventory for Men. VARIABLES PRINCIPALES Características demográficas y respuestas a los cuestionarios. RESULTADOS 574 farmacéuticos seleccionaron a 1.147 pacientes, de los cuales 1.113 fueron incluidos en el análisis. No se observaron diferencias estadísticas entre los grupos en cuanto al peso, la hipertensión, la diabetes mellitus, la hipercolesterolemia, la dislipidemia, la depresión o el estrés. Tampoco se observaron diferencias estadísticas respecto a la gravedad de la DE (p = 0,7892 ni a la proporción de hombres sin DE en cada grupo (p = 0,5755. En ambos grupos, los pacientes habían presentado síntomas de DE durante una media de veintiséis meses antes de la primera consulta a un profesional sanitario. Para el 60,2% de los pacientes incluidos en el grupo sin receta, la visita a la farmacia fue la primera ocasión en la que habían hablado de su DE con un profesional sanitario, y el 50% de aquellos que habían hablado previamente de la DE lo hab

  10. Effect of estradiol on planktonic growth, coaggregation, and biofilm formation of the Prevotella intermedia group bacteria.

    Science.gov (United States)

    Fteita, Dareen; Könönen, Eija; Söderling, Eva; Gürsoy, Ulvi Kahraman

    2014-06-01

    Alterations in the quantity and quality of biofilms at gingival margin are considered to play a role in the initiation and development of pregnancy-related gingivitis. Prevotella intermedia sensu lato is able to consume estradiol, the major sex hormone secreted during pregnancy, in the absence of vitamin K. The aim of the study was to examine the effect of estradiol on the planktonic growth, coaggregation, polysaccharide production, and biofilm formation of the P. intermedia group bacteria, namely P. intermedia, Prevotella nigrescens, and Prevotella pallens. In all experiments, the type strain (ATCC) and a clinical strain (AHN) of P. intermedia, P. nigrescens, and P. pallens were incubated with the concentrations of 0, 30, 90, and 120 nmol/L of estradiol. Planktonic growth was assessed by means of the colony forming unit method, while coaggregation and biofilm formation were assessed by spectrophotometric methods. In the determination of protein and polysaccharide levels, the Bradford and phenol-sulfuric acid methods were used, respectively. P. pallens AHN 9283 and P. nigrescens ATCC 33563 increased their numbers at planktonic stage with increasing estradiol concentrations. In 48-h biofilm tests, elevated protein levels were found for both strains of P. intermedia, and the strains P. nigrescens ATCC 33563 and P. pallens AHN 9283 in the presence of estradiol. The P. intermedia strains also increased the levels of polysaccharide formation in the biofilm. Coaggregation of the P. intermedia group organisms with Fusobacterium nucleatum was enhanced only in P. intermedia AHN 8290. In conclusion, our in vitro experiments indicate that estradiol regulates planktonic growth, coaggregation, polysaccharide production, and biofilm formation characteristics of P. intermedia, P. nigrescens, and P. pallens differently. These results may, at least partly, explain the differences seen in their contribution to the pathogenesis of pregnancy-related gingivitis

  11. Protective effect of an egg yolk-derived immunoglobulin (IgY) against Prevotella intermedia-mediated gingivitis.

    Science.gov (United States)

    Hou, Y-Y; Zhen, Y-H; Wang, D; Zhu, J; Sun, D-X; Liu, X-T; Wang, H-X; Liu, Y; Long, Y-Y; Shu, X-H

    2014-04-01

    To investigate the effects of an egg yolk-derived immunoglobulin (IgY) specific to Prevotella intermedia in vitro and in vivo. An IgY specific to P. intermedia was produced by immunizing hens with formaldehyde-inactivated P. intermedia and showed high titres when subjected to an ELISA. The obtained IgY inhibited the growth of P. intermedia in a dose-dependent manner at concentrations from 1 to 20 mg ml(-1) in Center for Disease Control and Prevention liquid medium. Forty rats were challenged with P. intermedia on gingivae and then randomly divided into four groups, which were syringed respectively with phosphate-buffered saline, 1 mg ml(-1) of tinidazole, 20 mg ml(-1) of nonspecific IgY and 20 mg ml(-1) of the IgY specific to P. intermedia at a dosage of 300 μl per day. Gingival index (GI), plaque index (PI), bleeding on probing (BOP), counts of white blood cell (WBC) and histopathological slide of the gums were measured after treatment for 15 days. The gingivitis rats treated with the IgY specific to P. intermedia showed significantly decreased GI, PI, BOP and WBC (P gingivitis. A new immunoglobulin specific to P. intermedia was developed from egg yolk. This specific IgY can dose-dependently inhibit the growth of P. intermedia and protect rats from gingivitis induced by P. intermedia. The new IgY has potential for the treatment of P. intermedia-mediated gingivitis. © 2013 The Society for Applied Microbiology.

  12. Tropical House Gecko (Hemidactylus mabouia predation on brown spiders (Loxosceles intermedia

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    E.N. Ramires

    2004-01-01

    Full Text Available Brown spiders (Loxosceles spp. are venomous arachnids, successfully adapted to urban habitats in Brazil. Loxoscelism became a serious public health problem in Paraná State, especially at the capital Curitiba, where the most abundant species is Loxosceles intermedia. Hemidactylus mabouia (Gekkonidae lizards are synanthropic predators of arthropods. In this paper, we describe the predatory behavior of the Tropical House Gecko H. mabouia on L. intermedia under laboratory conditions. Twelve geckos were observed, and all of them fed on brown spiders (n=123 observations. The attack consisted of a fast run followed by one bite on the spider’s abdomen or legs. The geckos did not attack L. intermedia anterior body parts, probably due to the fangs present in this region. Two Hemidactylus individuals were killed by L. intermedia bites: during a predatory encounter, and by an induced bite on a restrained lizard. The observations summarized in this paper show that H. mabouia could be used in the biological control of Loxosceles populations in human dwellings. However, additional field studies are necessary to quantify the impact of H. mabouia predation on urban populations of L. intermedia and other species of the same genus.

  13. Acoustic signals in the sand fly Lutzomyia (Nyssomyia intermedia (Diptera: Psychodidae

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    Peixoto Alexandre A

    2011-05-01

    Full Text Available Abstract Background Acoustic signals are part of the courtship of many insects and they often act as species-specific signals that are important in the reproductive isolation of closely related species. Here we report the courtship songs of the sand fly Lutzomyia (Nyssomyia intermedia, one of the main vectors of cutaneous leishmaniasis in Brazil. Findings Recordings were performed using insects from three localities from Eastern Brazil: Posse and Jacarepaguá in Rio de Janeiro State and Corte de Pedra in Bahia State. The three areas have remnants of the Brazilian Atlantic forest, they are endemic for cutaneous leishmaniasis and L. intermedia is the predominant sand fly species. We observed that during courtship L. intermedia males from all populations produced pulse songs consisting of short trains. No significant differences in song parameters were observed between the males of the three localities. Conclusions L. intermedia males produce acoustic signals as reported for some other sand flies such as the sibling species of the Lutzomyia longipalpis complex. The lack of differences between the males from the three localities is consistent with previous molecular studies of the period gene carried out in the same populations, reinforcing the idea that L. intermedia is not a species complex in the studied areas and that the three populations are likely to have similar vectorial capacities.

  14. Acoustic signals in the sand fly Lutzomyia (Nyssomyia) intermedia (Diptera: Psychodidae)

    Science.gov (United States)

    2011-01-01

    Background Acoustic signals are part of the courtship of many insects and they often act as species-specific signals that are important in the reproductive isolation of closely related species. Here we report the courtship songs of the sand fly Lutzomyia (Nyssomyia) intermedia, one of the main vectors of cutaneous leishmaniasis in Brazil. Findings Recordings were performed using insects from three localities from Eastern Brazil: Posse and Jacarepaguá in Rio de Janeiro State and Corte de Pedra in Bahia State. The three areas have remnants of the Brazilian Atlantic forest, they are endemic for cutaneous leishmaniasis and L. intermedia is the predominant sand fly species. We observed that during courtship L. intermedia males from all populations produced pulse songs consisting of short trains. No significant differences in song parameters were observed between the males of the three localities. Conclusions L. intermedia males produce acoustic signals as reported for some other sand flies such as the sibling species of the Lutzomyia longipalpis complex. The lack of differences between the males from the three localities is consistent with previous molecular studies of the period gene carried out in the same populations, reinforcing the idea that L. intermedia is not a species complex in the studied areas and that the three populations are likely to have similar vectorial capacities. PMID:21569534

  15. The effects of tetracycline, minocycline, doxycycline and ofloxacin on Prevotella intermedia biofilm.

    Science.gov (United States)

    Takahashi, N; Ishihara, K; Kimizuka, R; Okuda, K; Kato, T

    2006-12-01

    Prevotella intermedia, a black-pigmented, anaerobic, gram-negative bacterium, is associated with various type of periodontitis. Antibiotic treatments via a systemic or local route have been reported as being useful for treating periodontal disease. The purpose of this study was to examine the effects of four antibiotics, tetracycline (TET), minocycline (MINO), doxycycline (DOXY) and ofloxacin (OFLX) on P. intermedia biofilms at minimum inhibitory concentrations (MIC) from one-fold to 100-fold. MICs were determined for planktonic cells. Biofilm formation was determined with the crystal violet stain method and the bioactivities in the biofilms were determined with the adenosine triphosphate (ATP) -bioluminescent assay using a 96-well culture plate. At one-fold MIC, DOXY inhibited biofilm formation by P. intermedia ATCC 25611. Other antibiotics at one-fold MIC had no effects on the biofilm formation of tested bacterial strains. In P. intermedia ATCC 25611 biofilms, all the antibiotics tested showed inhibitory activities at five- to 100-fold MICs. In the biofilms of P. intermedia strains, except ATCC 25611, treated with three tetracycline antibiotics, the bioactivities were significantly increased, indicating the difficulties involved in designing antibiotic therapy for periodontal disease.

  16. Increase in probing depth is correlated with a higher number of Prevotella intermedia genotypes.

    Science.gov (United States)

    Alves, Ana Cláudia B A; Napimoga, Marcelo H; Klein, Marlise I; Hofling, Jose F; Gonçalves, Reginaldo B

    2006-01-01

    The aims of this study were to determine the genotypic diversity of Prevotella intermedia in subgingival plaque samples by using two techniques, arbitrarily primed polymerase chain reaction (AP-PCR) and heteroduplex analysis, and to assess the relationship of this diversity with increase in probing depth. The subgingival plaque samples were obtained from 12 patients using paper points inserted into periodontal pockets (diseased sites) and healthy gingival sulci (healthy sites) of the same subjects. After isolation and identification, AP-PCR was performed for genotypic characterization of P. intermedia (80 isolates). The clinical samples with a positive result for P. intermedia were amplified by 16S rRNA-based PCR method, and the amplicons were subjected to heteroduplex analysis. The agreement between the two methods was very high; the AP-PCR and heteroduplex analysis showed that subjects harbored between one and five distinct genotypes of P. intermedia, with a positive association between numbers of genotypes by AP-PCR (P = 0.0042) or heteroduplex (P = 0.0099) and increase in probing depth. No matching of P. intermedia genotypes was observed between healthy and diseased sites of the same individual. Interindividual analyses demonstrated absence of identical clones and indicated a high level of genetic diversity in the species. A clear relationship was observed between a higher number of genotypes and increase in probing depth; these results suggest that environmental challenges in the periodontal pockets may modulate the microbiota by selecting genotypes best able to exploit the environment.

  17. Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia.

    Science.gov (United States)

    Johnston, Christopher D; Skeete, Chelsey A; Fomenkov, Alexey; Roberts, Richard J; Rittling, Susan R

    2017-01-01

    Prevotella intermedia, a major periodontal pathogen, is increasingly implicated in human respiratory tract and cystic fibrosis lung infections. Nevertheless, the specific mechanisms employed by this pathogen remain only partially characterized and poorly understood, largely due to its total lack of genetic accessibility. Here, using Single Molecule, Real-Time (SMRT) genome and methylome sequencing, bisulfite sequencing, in addition to cloning and restriction analysis, we define the specific genetic barriers to exogenous DNA present in two of the most widespread laboratory strains, P. intermedia ATCC 25611 and P. intermedia Strain 17. We identified and characterized multiple restriction-modification (R-M) systems, some of which are considerably divergent between the two strains. We propose that these R-M systems are the root cause of the P. intermedia transformation barrier. Additionally, we note the presence of conserved Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) systems in both strains, which could provide a further barrier to exogenous DNA uptake and incorporation. This work will provide a valuable resource during the development of a genetic system for P. intermedia, which will be required for fundamental investigation of this organism's physiology, metabolism, and pathogenesis in human disease.

  18. Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia.

    Directory of Open Access Journals (Sweden)

    Christopher D Johnston

    Full Text Available Prevotella intermedia, a major periodontal pathogen, is increasingly implicated in human respiratory tract and cystic fibrosis lung infections. Nevertheless, the specific mechanisms employed by this pathogen remain only partially characterized and poorly understood, largely due to its total lack of genetic accessibility. Here, using Single Molecule, Real-Time (SMRT genome and methylome sequencing, bisulfite sequencing, in addition to cloning and restriction analysis, we define the specific genetic barriers to exogenous DNA present in two of the most widespread laboratory strains, P. intermedia ATCC 25611 and P. intermedia Strain 17. We identified and characterized multiple restriction-modification (R-M systems, some of which are considerably divergent between the two strains. We propose that these R-M systems are the root cause of the P. intermedia transformation barrier. Additionally, we note the presence of conserved Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR systems in both strains, which could provide a further barrier to exogenous DNA uptake and incorporation. This work will provide a valuable resource during the development of a genetic system for P. intermedia, which will be required for fundamental investigation of this organism's physiology, metabolism, and pathogenesis in human disease.

  19. Prevotella intermedia induces matrix metalloproteinase-9 expression in human periodontal ligament cells.

    Science.gov (United States)

    Guan, Su-Min; Shu, Lei; Fu, Shan-Min; Liu, Bin; Xu, Xiu-Li; Wu, Jun-Zheng

    2008-06-01

    Matrix metalloproteinases (MMPs) play pivotal roles in inflammatory diseases including chronic periodontitis. The effects of Prevotella intermedia, a major periodontal pathogen, on MMP-9 production in primary human periodontal ligament (hPDL) cells were examined in the present study. MMP-9 mRNA expression was measured by semiquantitative reverse transcriptase PCR and its protein secretion was assayed by gelatin zymography. Prevotella intermedia ATCC 25611 supernatant time and dose-dependently induced MMP-9 expression. In contrast, Porphyromanas gingivalis ATCC 33277 supernatants, Escherichia coli lipopolysacchride and IL-1beta exhibited no stimulatory effects on MMP-9 production in hPDL cells. Mitogen-activated protein kinases [MAPK, including extracellular signal-related kinases (ERK), c-jun N-terminal kinases (JNK) and p38] inhibitors exerted no effect on the P. intermedia-induced MMP-9 production, indicating that P. intermedia induced MMP-9 production through an MAPK-independent pathway. Our results demonstrated that P. intermedia may contribute to periodontal tissue destruction during chronic periodontitis by inducing MMP-9 production in hPDL cells.

  20. Intervención psicológica en la obesidad : Un estudio comparativo entre el enfoque cognitivo conductual y el enfoque humanista

    OpenAIRE

    Molina Vierge, Patricio

    2014-01-01

    En este estudio se compara la intervención psicológica por excelencia en casos de obesidad y sobrepeso, la intervención cognitivoconductual, con la intervención humanista. Mediante el seguimiento de tres grupos de investigación a los que se prescriben restricciones propias de una dieta hipocalórica, uno sometido a terapia humanista individual, otro sometido a terapia cognitivoconductual grupal y un tercero sometido a una formación nutricional (grupo de cuasicontrol), este estudio pretende det...

  1. Gestalt-Terapia e Terapia Sistêmica: o corpo em psicoterapia

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    Marília Toscano de Araujo Gurgel

    2008-06-01

    Full Text Available Este trabalho demonstra a contribuição da abordagem corporal da Gestalt-Terapia para a Terapia Familiar Sistêmica. O método utilizado foi a gravação de uma oficina de corpo estruturada em saberes das duas abordagens. A oficina proporcionou, a cada participante, maior qualidade de atenção, presença, contato e criatividade, uma forma mais integrada de construir sua identidade. O entrecruzamento das duas abordagens trouxe às pessoas maior consciência de quem são e como são no "mundo", ajudando-as no processo de assunção da história que contam. A experiência mostrou aos terapeutas as possibilidades que o trabalho corporal oferece como método de apreensão da realidade co-construída.

  2. A terapia cognitivo-comportamental no transtorno obsessivo-compulsivo

    OpenAIRE

    Cordioli,Aristides Volpato

    2008-01-01

    OBJETIVO: Descrever a terapia cognitivo-comportamental no tratamento dos sintomas do transtorno obsessivo-compulsivo. MÉTODO: Por meio da revisão de artigos e livros-texto, descrever as origens e os fundamentos da terapia cognitivo-comportamental no tratamento dos sintomas do transtorno obsessivo-compulsivo. Por meio da revisão no Medline de ensaios clínicos randomizados e metanálises, apontar as evidências de eficácia dessa modalidade de tratamento. RESULTADOS E CONCLUSÕES: A terapia cogniti...

  3. ARTE TERAPIA: HERRAMIENTA DE AYUDA EN LA ESCUELA

    OpenAIRE

    Alcázar Ruiz de Peralta, Karla Katherin

    2017-01-01

    La investigación tiene como Objetivo: incluir el arte terapia en la escuela, permitiendo que el docente de arte logre conocer el proceso de arte terapia de manera detallada, saber sus fundamentos, entender sus beneficios y ventajas, con la finalidad de poder aplicarla en el futuro como una herramienta de ayuda en la escuela. Método: la investigación es de diseño no experimental, de tipo descriptivo-analítico. Enfoque cualitativo. Conclusión: el Arte terapia, por ser un proceso adecuado para t...

  4. Terapia dialéctico conductual para el trastorno de personalidad límite

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    Irene de la Vega

    2013-06-01

    Full Text Available La terapia dialéctico conductual (TDC es una terapia psicológica de tercera generación desarrollada por Marsha Linehan (1993a, 1993b para tratar específicamente los síntomas característicos del trastorno límite de la personalidad: inestabilidad afectiva, trastorno de identidad, impulsividad y dificultades en las relaciones sociales. La TDC parte de un modelo biosocial del trastorno límite y combina técnicas cognitivo conductuales con aproximaciones zen y budistas basadas en la aceptación de la realidad tal como se presenta. El tratamiento consiste en un entrenamiento en habilidades, psicoterapia individual y atención en crisis y es llevado a cabo por un equipo coordinado de profesionales que ejercen distintas funciones. La TDC cuenta con estudios de eficacia y se está aplicando con éxito a otras poblaciones de pacientes con elevada impulsividad.

  5. Conforto na perspectiva de familiares de pessoas internadas em Unidade de Terapia Intensiva

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    Kátia Santana Freitas

    2012-01-01

    Full Text Available Se objetivó con el estudio comprender el significado de confort para familiares de personas en Unidad de Terapia Intensiva. Esta investigación cualitativa fue realizada en la Unidad de Terapia Intensiva general de un gran hospital, en Salvador-Bahia, Brasil. Catorce familiares fueron sometidos a la encuesta. Se utilizó la teoría del Interaccionismo Simbólico y se empleó la técnica de análisis de datos cualitativos. Las categorías Seguridad, Acogida, Información, Apoyo social y espiritual, Proximidad, Comodidad e Integración consigo y con el cotidiano expresaron los significados de confort que involucró la confianza de la competencia técnico científica y actitud solidaria y sensible del equipo, oportunidad de recuperación, acceso a la información y posibilidad de acercarse al pariente, apoyo de personas del entorno social, de fuentes espirituales y de la estructura ambiental del hospital y preservación del cuidado personal y actividades habituales. Se concluye que la familia es importante como objeto y sujeto de acciones en salud y debe estar enfocada en las políticas y programas de salud pública en Brasil.

  6. FUNÇÕES DESEMPENHADAS PELO ENFERMEIRO EM UNIDADE DE TERAPIA INTENSIVA

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    Micheli Grande Cenedési

    2012-01-01

    Full Text Available Estudio exploratorio, con enfoque cualitativo con objetivo general de identificar las funciones desarrolladas por enfermero en la Unidad de Terapia Intensiva, siguiendo el marco canadiense de función del enfermero (atención, educar, coordinar, supervisar y colaborar. Los datos fueron recolectados en 2009 mediante aplicación de cuestionario y observación sistemática de nueve enfermeros de una unidad de terapia intensiva de un hospital privado de Curitiba-PR, Brasil. Los datos obtenidos fueron clasificados por la técnica de análisis de contenido. Los resultados indican que los enfermeros son en su mayoría mujeres jóvenes, que no tienen experiencia en la unidad de cuidados intensivos. La observación de las actividades presenta que todas las funciones descritas en el marco son desarrolladas por los enfermeros del servicio, donde las funciones de coordinación, supervisión y atención técnica son las más significativas.

  7. Terapia musical en rehabilitación: una revisión narrativa (2004-2014

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    María del Carmen Gómez Álvaro

    2015-04-01

    Full Text Available Las investigaciones en el campo de la aplicación de la terapia musical como herramienta terapéutica han aumentado en los últimos diez años. Las revisiones narrativas publicadas hasta la fecha muestran los efectos y la eficacia de la terapia musical como estrategia de intervención rehabilitadora. Sin embargo, no tratan la temática desde una perspectiva general y ninguna de ellas ha incluido el término “musical therapy” entre los términos de búsqueda de literatura, quedando excluidos, por tanto, los resultados de toda una serie de estudios que han contribuido a esclarecer la eficacia de la musicoterapia en rehabilitación. Por estas razones, el objetivo de este trabajo es remediar esta laguna y revisar los hallazgos de investigaciones publicadas en los últimos diez años que han quedado excluidas de revisiones anteriores. Específicamente, esta contribución pretende responder a las preguntas de investigación: ¿Qué tipo de trastornos se pueden beneficiar eficazmente de la musicoterapia? y ¿Qué efectos positivos tiene la musicoterapia en rehabilitación? Para responder a estas preguntas, se llevó a cabo una búsqueda electrónica de literatura en las bases de datos Academic Search Complete, Medline y Science Direct. La búsqueda incluyó el término “musical therapy” y ciertos criterios de inclusión, tales como los reportes sobre estudios empíricos redactados en que utilizaran música como estimulación terapéutica. Como resultado, se revisaron en total veinticuatro estudios, de los que se analizaron una serie de elementos, tales como los grupos de participantes (experimental vs. control, los resultados obtenidos y las limitaciones. Como conclusión, afirmamos, con cautela, que la musicoterapia puede contribuir a la rehabilitación de las funciones cognitivas, motoras y sensoriales del daño cerebral; a la rehabilitación de la esquizofrenia y de la depresión primaria; a la mejoría de los trastornos neurodegenerativos

  8. Paisajes del delito y la inseguridad en las ciudades intermedias

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    Quim Bonastra

    2011-02-01

    Full Text Available Until very recently, phenomena like delinquency were only considered dysfunctions of the system. However, nowadays this phenomenon has become inherent to its way of functioning. This dynamic is influenced by factors such as the increasing polarization of developed economies or the enormous masses of displaced people, due to either economic or political reasons, from developing countries who, in rich countries, often fall in situations of precariousness. In the following text we will see how all these pieces are interrelated in an intermediate city, and we will analyse the fluctuation of violent or intimidating thefts, as well as the most common places where these are committed.Si hasta hace bien poco se había considerado que fenómenos como la delincuencia no eran más que disfunciones del sistema, en el mundo en el que vivimos este fenómeno se ha convertido en inherente a su manera de funcionar. Influyen en esta dinámica factores como la polarización creciente de las economías desarrolladas o las ingentes masas de desplazados, ya sea por razones económicas o políticas, de países en vías de desarrollo que, en los países ricos, caen las más de las veces en la precariedad. En el texto que sigue se verá como se engarzan todas estas piezas en una ciudad intermedia y se analizará la fluctuación de los robos con violencia o intimidación, así como los lugares típicos en los que éstos se cometen.DOWNLOAD THIS PAPER FROM SSRN: http://ssrn.com/abstract=1739302

  9. Serum Prohepcidin Levels in Children with Thalassemia Major and Intermedia

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    Celil Yılmaz

    2017-04-01

    Full Text Available Objective: To determine the role of hepcidin hormone levels in iron accumulation in patients with thalassemia major (TM and thalassemia intermedia (TI. Materials and Methods: Serum prohepcidin and ferritin levels were determined in 34 patient with TM, 10 patient with TI, who attended the Department of Pediatric Hematology Adnan Menderes University Medical Faculty and the Department of Pediatrics at Aydın Atatürk State Hospital between 1 September 2006 and 30 September 2007 and 40 control patients without infection/inflammation, hepatitis or liver failure. Serum prohepcidin levels were measured using a commercial enzyme-linked immunosorbent assay kit (DRG International, Inc. Marburg, Germany; ferritin was studied with chemiluminescence method (Immulite 2000 DPC. Results: Mean serum ferritin levels in TM, TI and control groups were 2347.97±1724.81 ng/mL (range: 144-8015 ng/mL, 1352.40±918.94 ng/mL (range: 311-3109 ng/mL, and 33.35±12.03 ng/mL (range: 20-69.1 ng/mL, respectively. Serum prohepcidin levels in the same groups were 221.78±74.38 ng/mL (range: 7l.14-446.57 ng/mL, 173.31±52.14 ng/mL (range: 100.83-267.69 ng/mL, and 218.20±50.37 ng/mL (range: 116.18-330.43 ng/mL, respectively. There was a statistically significant difference in prohepcidin levels between patients with TI and control group only (p=0.016. No correlation was found between prohepcidin and ferritin levels in all groups (r=-0.023, p=0.839. Conclusion: Low levels of prohepcidin in patients with TI may be related to increased erythropoietic activity. Prohepcidin can be an indicator of active erythropoiesis.

  10. Composition, Cytotoxic and Antimicrobial Activities of Satureja intermedia C.A.Mey Essential Oil.

    Science.gov (United States)

    Sharifi-Rad, Javad; Sharifi-Rad, Mehdi; Hoseini-Alfatemi, Seyedeh Mahsan; Iriti, Marcello; Sharifi-Rad, Majid; Sharifi-Rad, Marzieh

    2015-08-03

    In this study, the essential oil (EO) constituents from the aerial parts of Satureja intermedia C.A.Mey were detected by GC and GC/MS. The antimicrobial activity of EO on oral pathogens and its cytotoxicity to human cancer cells were determined by the microbroth dilution method and the crystal violet staining method, respectively. Thirty-nine compounds were identified and the main EO constituents were γ-terpinene (37.1%), thymol (30.2%), p-cymene (16.2%), limonene (3.9%), α-terpinene (3.3%), myrcene (2.5%), germacrene B (1.4%), elemicine (1.1%) and carvacrol (0.5%). The S. intermedia EO showed a concentration-dependent decrease in viability of Hep-G2 (hepatocellular carcinoma) and MCF-7 (breast adenocarcinoma) human cancer cell lines (p safety of S. intermedia EO as a new promising anticancer agent.

  11. Digital Distribution of Advertising for Publications (DDAP): a graphic arts prototype of electronic intermedia publishing (EIP)

    Science.gov (United States)

    Dunn, Patrice M.

    1998-01-01

    The Digital Distribution of Advertising for Publications (DDAP) is a graphic arts industry prototype of Electronic Intermedia Publishing (EIP). EIP is a strategic, multi- industrial concept that seeks to enable the capture and input of volumes of data (i.e., both raster and object oriented data -- as well as the latter's antecedent which is vector data -- color data and black-and-white data) from a multiplicity of devices; then flowing, controlling, manipulating, modifying, storing, retrieving, transmitting, and shipping, that data through an industrial process for output to a multiplicity of output devices (e.g., ink on paper, toner on paper, bits and bytes on CD ROM, Internet, Multimedia, HDTV, etc.). As the technical requirements of the print medium are among the most rigorous in the Intermedia milieu the DDAP prototype addresses some of the most challenging issues faced in Electronic Intermedia Publishing (EIP).

  12. Prevotella intermedia and Porphyromonas gingivalis in dental caries with periapical granuloma

    Directory of Open Access Journals (Sweden)

    Risya Cilmiaty

    2013-12-01

    Full Text Available Background: Dental caries with necrotic pulp is a multifactorial disease that attacks enamel involving tooth pulp. The anaerobic bacteria infection in the pulp chamber could induce the formation of periapical granuloma. However, the presence of the most frequently anaerobic bacteria identified in apical periodontitis, Porphyromonas gingivalis and Prevotella intermedia, in periapical granuloma have not been confirmed. Purpose: The aims of study were to determine the presence of Porphyromonas gingivalis and Prevotella intermedia in dental caries with necrotic pulp and to determine its relation to periapical granuloma. Methods: Thirty-six patients of dental caries with necrotic pulp in Dr. Moewardi General Hospital in Surakarta, Indonesia were involved and classified into two groups, the group of patients with periapical granuloma and the group of patients without periapical granuloma. The caries tooth was extracted, and the chronic periapical tissue was swabbed and cultured on blood agar medium in anaerobic condition. The bacterial DNA was extracted from the positive cultures and subjected for Polymerase Chain Reaction (PCR. Results: Periapical granuloma was more likely found in women (OR 5.5, 95% CI=1.277-23.693; RR 2.5, 95% CI= 1.025-6.100. Black colonies bacteria were associated with periapical granuloma (OR 2.2, 95% CI=0.517-9.594; RR 1.5, 95% CI=0.655-3.623. Porphyromonas gingivalis and Prevotella intermedia were detected in group with or without periapical granuloma, however, only Prevotella intermedia was associated with periapical granuloma (OR 1.6, 95% CI=0.418-5.903; RR 1.3, 95% CI=0.653-2.393. Conclusion: The presence of Porphyromonas gingivalis and Prevotella intermedia in periapical granuloma were confirmed, however, only Prevotella intermedia were associated with periapical granuloma.Latar belakang: Karies gigi dengan pulpa nekrosis adalah penyakit multifaktorial yang menyerang enamel hingga ruang pulpa gigi. Infeksi bakteri anaerob

  13. La terapia de la coherencia: un efonque constructivista apoyado por la neurociencia contemporánea

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    Michał Jasiński

    Full Text Available Resumen En el presente artículo se describe la terapia de la coherencia (TC. Con el fin de presentar este enfoque se realiza una breve descripción del constructivismo y su influencia tanto en la TC, como en la psicoterapia en general. Se continúa con la explicación detallada de la historia, teoría y metodología de la TC. Se describe también la reconsolidación de la memoria, un fenómeno descubierto recientemente en el campo de la neurociencia que parece ocurrir en el proceso de la TC y se presentan sus implicaciones para la psicoterapia. Se utiliza un breve estudio de caso para explicar el proceso de la TC de forma práctica. Finalmente, se describe el estado acual y el futuro de este enfoque terapéutico.

  14. Evolución de estrellas enanas blancas de Helio de masa baja e intermedia

    Science.gov (United States)

    Althaus, L. G.; Benvenuto, O. G.

    Numerosas observaciones realizadas particularmente en los últimos dos años parecen confirmar que las enanas blancas (EB) de helio de masa baja e intermedia son el resultado de la evolución de sistemas binarios cercanos. Con el objeto de realizar una adecuada interpretación de estos objetos son necesarios modelos de EBs de helio lo más detallado posibles. En este estudio presentamos cálculos detallados de la evolución de EBs de helio con masas entre M=0.1Msolar y M=0.5Msolar a intervalos de 0.05Msolar . Para ello, hemos tenido en cuenta los efectos de temperatura finita mediante un código de evolución estelar lo más actualizado posible. En particular, el transporte de energía es descripto en el marco del nuevo modelo para la convección turbulenta desarrollado por Canuto - Mazzitelli. Además hemos considerado la nueva ecuación de estado para plasmas de helio de Saumon et al. y nuevas opacidades radiativas OPAL. Las pérdidas por neutrinos fueron asimismo tenidas en cuenta. Excepto para las EBs más masivas, nuestros modelos iniciales están ubicados en las cercanías de la correspondiente línea de Hayashi para configuraciones de helio. Nuestros resultados muestran que existe una región prohibida en el diagrama observacional HR donde ninguna EB de helio puede encontrarse. Dicha región es para log{(L/Lsolar )}>= -0.25 and log{Teff} >= 4.45. Hemos encontrado también que los tracks evolutivos en el diagrama HR en el dominio de alta luminosidad (pre - EB) son fuertemente afectados por la eficiencia convectiva y que las pérdidas por neutrinos son importantes en los modelos más masivos. Finalmente hemos analizado la estructura de la zona convectiva externa encontrando que la teoría de Canuto - Mazzitelli conduce a un perfil convectivo muy diferente del dado por cualquier versión de la popular teoría de la mixing length. Si bién este comportamiento es decisivo en el contexto de las inestabilides pulsacionales, los radios y gravedades superficiales de

  15. Terapia cognitivo-conductual y psicosis

    Directory of Open Access Journals (Sweden)

    CARLOS CUEVAS-YUST

    2002-01-01

    Full Text Available En una patología, la psicosis, tradicionalmente reservada al tratamiento psiquiátrico, resulta especialmente interesante y prometedora la aparición de las intervenciones psicológicas. De hecho, la terapia cognitivo-conductual (TCC está mostrando ser una aproximación efectiva en el tratamiento de síntomas psicóticos positivos resistentes a la medicación neuroléptica, existiendo incluso alguna evidencia de que podría contribuir a la reducción de las recaídas. En el presente trabajo describimos las etapas de la TCC en la psicosis, así como su adaptación a esta patología.

  16. Direct quantitative differentiation between Prevotella intermedia and Prevotella nigrescens in clinical specimens.

    Science.gov (United States)

    Gmür, Rudolf; Thurnheer, Thomas

    2002-05-01

    This paper describes a quantitative fluorescent in situ hybridization (FISH) assay for the differential identification of Prevotella intermedia and Prevotella nigrescens in clinical samples, and compares its performance with less discriminatory culture and quantitative immunofluorescence (IF) assays. Fluorescence-labelled oligonucleotide probes directed to specific 16S rRNA sequences of P. intermedia, P. nigrescens, Prevotella pallens and Prevotella denticola were hybridized under stringent conditions with cultured reference strains or plaque samples from deep periodontal pockets. Probe specificity was defined with strains from multiple oral Prevotella species. The lower detection level of the assays was approximately 3x10(3) target cells per ml of plaque-sample suspension. P. intermedia, P. nigrescens, P. pallens and P. denticola were detected in plaques with prevalences of 69, 67, 0 and 28%, respectively. On average, 3.9 x 10(6) P. intermedia, 3.1 x 10(6) P. nigrescens and 5.6 x 10(5) P. denticola cells were counted per positive sample. All three species were found almost exclusively in dense mixed aggregates. Quantitative FISH data agreed satisfactorily with corresponding IF data (r=0.711). Both FISH and IF enumerations of the sum of P. intermedia and P. nigrescens markedly exceeded the c.f.u. counts of black-pigmented colonies in Porphyromonas gingivalis-free cultured subgingival plaques. The results demonstrate the validity of this new assay. Unlike established IF, culture, PCR or checkerboard DNA hybridization assays, this FISH assay differentiates quantitatively between P. intermedia and P. nigrescens, provides visual accuracy control, and offers insights into the spatial distribution of the target cells within a clinical sample.

  17. Interpain A, a cysteine proteinase from Prevotella intermedia, inhibits complement by degrading complement factor C3.

    Science.gov (United States)

    Potempa, Michal; Potempa, Jan; Kantyka, Tomasz; Nguyen, Ky-Anh; Wawrzonek, Katarzyna; Manandhar, Surya P; Popadiak, Katarzyna; Riesbeck, Kristian; Eick, Sigrun; Blom, Anna M

    2009-02-01

    Periodontitis is an inflammatory disease of the supporting structures of the teeth caused by, among other pathogens, Prevotella intermedia. Many strains of P. intermedia are resistant to killing by the human complement system, which is present at up to 70% of serum concentration in gingival crevicular fluid. Incubation of human serum with recombinant cysteine protease of P. intermedia (interpain A) resulted in a drastic decrease in bactericidal activity of the serum. Furthermore, a clinical strain 59 expressing interpain A was more serum-resistant than another clinical strain 57, which did not express interpain A, as determined by Western blotting. Moreover, in the presence of the cysteine protease inhibitor E64, the killing of strain 59 by human serum was enhanced. Importantly, we found that the majority of P. intermedia strains isolated from chronic and aggressive periodontitis carry and express the interpain A gene. The protective effect of interpain A against serum bactericidal activity was found to be attributable to its ability to inhibit all three complement pathways through the efficient degradation of the alpha-chain of C3 -- the major complement factor common to all three pathways. P. intermedia has been known to co-aggregate with P. gingivalis, which produce gingipains to efficiently degrade complement factors. Here, interpain A was found to have a synergistic effect with gingipains on complement degradation. In addition, interpain A was able to activate the C1 complex in serum, causing deposition of C1q on inert and bacterial surfaces, which may be important at initial stages of infection when local inflammatory reaction may be beneficial for a pathogen. Taken together, the newly characterized interpain A proteinase appears to be an important virulence factor of P. intermedia.

  18. Interpain A, a cysteine proteinase from Prevotella intermedia, inhibits complement by degrading complement factor C3.

    Directory of Open Access Journals (Sweden)

    Michal Potempa

    2009-02-01

    Full Text Available Periodontitis is an inflammatory disease of the supporting structures of the teeth caused by, among other pathogens, Prevotella intermedia. Many strains of P. intermedia are resistant to killing by the human complement system, which is present at up to 70% of serum concentration in gingival crevicular fluid. Incubation of human serum with recombinant cysteine protease of P. intermedia (interpain A resulted in a drastic decrease in bactericidal activity of the serum. Furthermore, a clinical strain 59 expressing interpain A was more serum-resistant than another clinical strain 57, which did not express interpain A, as determined by Western blotting. Moreover, in the presence of the cysteine protease inhibitor E64, the killing of strain 59 by human serum was enhanced. Importantly, we found that the majority of P. intermedia strains isolated from chronic and aggressive periodontitis carry and express the interpain A gene. The protective effect of interpain A against serum bactericidal activity was found to be attributable to its ability to inhibit all three complement pathways through the efficient degradation of the alpha-chain of C3 -- the major complement factor common to all three pathways. P. intermedia has been known to co-aggregate with P. gingivalis, which produce gingipains to efficiently degrade complement factors. Here, interpain A was found to have a synergistic effect with gingipains on complement degradation. In addition, interpain A was able to activate the C1 complex in serum, causing deposition of C1q on inert and bacterial surfaces, which may be important at initial stages of infection when local inflammatory reaction may be beneficial for a pathogen. Taken together, the newly characterized interpain A proteinase appears to be an important virulence factor of P. intermedia.

  19. Stress in nurses working in intensive care units El estrés de los enfermeros que actúan en una unidad de terapia intensiva Estresse de enfermeiros com atuação em unidade de terapia intensiva

    Directory of Open Access Journals (Sweden)

    Ana Maria Cavalheiro

    2008-02-01

    Full Text Available The present study aimed to identify the presence of stress in nurses working in intensive care units, the stressing agents and symptoms associated to the nurses' perceptions of stress, and to assess the correlation between the occurrence of stress, sources of stress, and symptoms shown by the nurses. Seventy-five nurses took part in the study. The data were collected from questionnaires, analyzed with the Pearson correlation coefficients, and adjusted by general linear models. The study showed the presence of stress related to work dissatisfaction, activities regarded as critical situations in intensive care units, symptoms related to cardiovascular, digestive and musculoskeletal disorders. The conclusion is that stress is present in nurses' activities in intensive care units, related to characteristics of the health area itself, causing dissatisfaction and stress-related symptoms.El presente estudio tuvo como objetivos: identificar la presencia de estrés en enfermeros que trabajan en unidades de terapia intensiva; identificar a los agentes estresantes y a los síntomas asociados al estrés, según la percepción del enfermero; y, evaluar la correlación entre la presencia del estrés, las fuentes del estrés y los síntomas presentados por los enfermeros. Setenta y cinco enfermeros participaron del estudio. Los datos fueron recolectados por medio de cuestionarios. El análisis fue realizado utilizando los coeficientes de correlación de Pearson y ajustando con modelos lineales generalizados. El estudio mostró la presencia de estrés correlacionado a: la insatisfacción en el trabajo; a las actividades consideradas como situaciones críticas en unidad de terapia intensiva; a los síntomas relacionados con alteraciones cardiovasculares; al aparato digestivo y a los músculos del esqueleto. La conclusión del estudio es que el estrés está presente en la actividad del enfermero en las unidades de terapia intensiva y está correlacionado a

  20. Ambiente Robótico Lúdico para Terapias de Rehabilitación de Pacientes Pediátricos con Lesión del Miembro Superior

    Directory of Open Access Journals (Sweden)

    Jairo A. Acevedo-Londoño

    2018-03-01

    Full Text Available Este trabajo presenta una propuesta de asistencia para las terapias de rehabilitación de niños con lesión del miembro superior, basada en el desarrollo de un ambiente lúdico de asistencia tecnológica, cuyo objetivo es contribuir al aumento del beneficio de las terapias, en cuanto a la motivación y avance en el componente de rehabilitación físico. Este ambiente se denominó SpinBOT, y está compuesto por:  un guante didáctico instrumentado con un acelerómetro, un módulo de control, una plataforma robótica y una herramienta de visualización. Se seleccionaron 4 sujetos de estudio, divididos en dos grupos, grupo objetivo y grupo de control. La intervención de la terapia consistió en el desarrollo de 3 tareas durante 4 semanas y 2 sesiones por semana. Mediante la valoración GAS, (del inglés: “Goal Attainment Scale” se observó el progreso del uso operativo del robot, lo que ayudó en la motivación para asistir a las terapias; además de, generar habilidades físicas y cognitivas.

  1. Incomplete paraplegia caused by extramedullary hematopoiesis in a patient with thalassemia intermedia.

    Science.gov (United States)

    Hisamud-Din, Nurhasyimah; Mustafah, Nadia Mohd; Fauzi, Aishah Ahmad; Hashim, Natiara Mohamad

    2017-01-01

    Extramedullary hematopoiesis (EMH) is the production of blood cell precursors outside the bone marrow that occur in various hematological diseases. In patients with thalassemia intermedia, ineffective erythropoiesis drives compensatory EMH in the liver, pancreas, pleura, spleen, ribs and spine. We describe a patient with thalassemia intermedia who presented with acute neurological symptoms caused by paraspinal EMH, which responded well to combination therapy of steroid, hypertransfusion, laminectomy and excision of pseudotumor and hydroxyurea therapy to boost the formation of fetal haemoglobin. Prompt recognition of EMH based on clinical presentation and typical radiological findings should be made. Early treatment is recommended to prevent irreversible damage to the spinal cord.

  2. Interactions of clinical isolates of Prevotella intermedia and Prevotella nigrescens with Porphyromonas gingivalis in biofilm formation.

    OpenAIRE

    Graziela Murta Barbosa

    2013-01-01

    Prevotella intermedia e Prevotella nigrescens são espécies comumente associadas Porphyromonas gingivalis. os objetivos foram verificar a co-agregação entre cepas de P. intermedia, P. nigrescens e P. gingivalis; quantificar a biomassa, avaliar a proporção dos microrganismos nos biofilmes mistos; verificar a interferência do co-cultivo pela técnica de dois compartimentos, avaliar os biofilmes em ensaios de Hibridização In Situ (FISH) e verificar o papel dos genes PINA0102 e PIN0398 de P. interm...

  3. Terapias cognitivo-conductuales en la fobia social, en adultos

    OpenAIRE

    San Vicente Montalbán, Ainara

    2015-01-01

    La fobia social es una enfermedad psiquiátrica que causa costes socioeconómicos significativos y que presenta una alta comorbilidad. Las terapias cognitivo-conductuales (TCC) pueden ser una alternativa de tratamiento efectiva, ya que buscan el cambio de pensamientos y comportamientos adquiridos en una enfermedad con un alto componente de aprendizaje. Objetivo: Verificar la efectividad de las terapias cognitivo-conductuales (TCC) con respecto a otros tratamientos empleados en la fobia soci...

  4. El enfoque sistémico en terapia familiar

    Directory of Open Access Journals (Sweden)

    Roxana Zevallos Vega

    1993-12-01

    Full Text Available La autora resalta algunos aspéctos de la aparición de la Terapia Familiar y describe las etapas por las que ha evolucionado, destacando el rol de autores como Hoffman, Minuchin, Maturana, entre otros. Se indica los inicios de la Terapia Familiar en el Perú, mencionándose la creación de grupos de profesionales que trabajan en este campo.

  5. El enfoque sistémico en terapia familiar

    OpenAIRE

    Roxana Zevallos Vega

    1993-01-01

    La autora resalta algunos aspéctos de la aparición de la Terapia Familiar y describe las etapas por las que ha evolucionado, destacando el rol de autores como Hoffman, Minuchin, Maturana, entre otros. Se indica los inicios de la Terapia Familiar en el Perú, mencionándose la creación de grupos de profesionales que trabajan en este campo.

  6. Aproximaciones proteómicas para el estudio del envejecimiento

    OpenAIRE

    Fanjul Hevia, Víctor

    2014-01-01

    La población mundial está envejeciendo progresivamente y la edad es el factor principal de riesgo de enfermedad cardiovascular (ECV), que a su vez es el principal problema de salud en países desarrollados. Aún no se conocen en profundidad los mecanismos moleculares que provocan el envejecimiento ni aquéllos que originan la ECV, y su estudio podría conducir hacia el desarrollo de estrategias preventivas o de terapias que alarguen significativamente la vida y mejoren su calidad. La progeria ...

  7. Modificación de la personalidad mediante una terapia cognitivo-conductual de afrontamiento al estrés

    Directory of Open Access Journals (Sweden)

    Julia Linares-Ortiz

    2014-01-01

    Full Text Available El objetivo de este estudio ha sido comprobar la posibilidad de modulación de variables de personalidad (tales como algunos de los Cinco Grandes Factores, o la personalidad resistente a través de la aplicación de un programa de afrontamiento del estrés. Para ello, han participado 26 personas del ámbito universitario con alto estrés percibido, distribuidas en dos grupos (grupo de tratamiento y grupo de control. Los instrumentos de evaluación seleccionados se clasificaron en dos grupos: variables psicológicas y emocionales y variables de personalidad. Los resultados encontrados mediantes un ANOVA de medidas repetidas mostraron que existía interacción momento x grupo en las variables optimismo, extraversión y responsabilidad, correspondientes al Modelo de los Cinco Grandes Factores, mostrando un incremento de las puntuaciones en estas variables en el grupo terapia y no encontrándose dicha interacción en el grupo control. Las puntuaciones en los componentes de neuroticismo, amabilidad, apertura a la experiencia y personalidad resistente no se modificaron en ninguno de los dos grupos. Este estudio apoya la idea de que modificando determinados parámetros emocionales relacionados con el estrés mediante terapia cognitivo-conductual se pueden ver modulados algunos factores de personalidad.

  8. Evaluation of the efficacy of vacuum cleaners for the integrated control of brown spider Loxosceles intermedia

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    E. N. Ramires

    2007-01-01

    Full Text Available Some venomous spiders of the genus Loxosceles can reach high population densities inside and around houses. In Brazil, most spider accidents are related to Loxosceles intermedia. Control of loxoscelism should utilize integrated pest management tools, such as vacuum cleaners, to eliminate egg sacs, webs and spiders. The present study tested the efficacy of one type of vacuum cleaner (for professional and domestic use in the control of L. intermedia populations. Cockroaches (Pycnoscelus surinamensis were used in some tests for comparison. Vacuuming using standard accessories or a paper tube resulted in the death of all female (n=60, male (n=60, young (n=60 and just-hatched (n=60 L. intermedia, and all egg sacs (n=5 were destroyed. The removal of the plastic plate present at the bottom of the vacuuming tube inside the machine allowed some spiders to survive the vacuuming process. When kept inside a vacuum bag full of dust and debris, adult females (n=10 survived for 10 days; however, significant mortality was observed among male (n=10 and young individuals (n=10. Addition of cornstarch to the vacuum bag did not affect the spiders (n=20. Vacuum cleaners, such as the one used in the present investigation, are promising tools for integrated management of L. intermedia and other spiders in domestic environments.

  9. Reconstruction of chromosome rearrangements between the two most ancestral duckweed species Spirodela polyrhiza and S. intermedia.

    Science.gov (United States)

    Hoang, Phuong T N; Schubert, Ingo

    2017-12-01

    The monophyletic duckweeds comprising five genera within the monocot order Alismatales are neotenic, free-floating, aquatic organisms with fast vegetative propagation. Some species are considered for efficient biomass production, for life stock feeding, and for (simultaneous) wastewater phytoremediation. The ancestral genus Spirodela consists of only two species, Spirodela polyrhiza and Spirodela intermedia, both with a similar small genome (~160 Mbp/1C). Reference genome drafts and a physical map of 96 BACs on the 20 chromosome pairs of S. polyrhiza strain 7498 are available and provide useful tools for further evolutionary studies within and between duckweed genera. Here we applied sequential comparative multicolor fluorescence in situ hybridization (mcFISH) to address homeologous chromosomes in S. intermedia (2n = 36), to detect chromosome rearrangements between both species and to elucidate the mechanisms which may have led to the chromosome number alteration after their evolutionary separation. Ten chromosome pairs proved to be conserved between S. polyrhiza and S. intermedia, the remaining ones experienced, depending on the assumed direction of evolution, translocations, inversion, and fissions, respectively. These results represent a first step to unravel karyotype evolution among duckweeds and are anchor points for future genome assembly of S. intermedia.

  10. Rhamnus intermedia Steud. et Hochst. - a New Source of Bioactive Phytochemicals

    Czech Academy of Sciences Publication Activity Database

    Samec, D.; Kremer, D.; Grúz, Jiří; Grubešić, R. J.; Piljac-Žegarac, J.

    2012-01-01

    Roč. 85, č. 2 (2012), s. 125-129 ISSN 0011-1643 R&D Projects: GA AV ČR KAN200380801 Institutional research plan: CEZ:AV0Z50380511 Keywords : Rhamnus intermedia * antioxidant activity * total phenols Subject RIV: EC - Immunology Impact factor: 0.614, year: 2012

  11. Composition, Cytotoxic and Antimicrobial Activities of Satureja intermedia C.A.Mey Essential Oil

    Directory of Open Access Journals (Sweden)

    Javad Sharifi-Rad

    2015-08-01

    Full Text Available In this study, the essential oil (EO constituents from the aerial parts of Satureja intermedia C.A.Mey were detected by GC and GC/MS. The antimicrobial activity of EO on oral pathogens and its cytotoxicity to human cancer cells were determined by the microbroth dilution method and the crystal violet staining method, respectively. Thirty-nine compounds were identified and the main EO constituents were γ-terpinene (37.1%, thymol (30.2%, p-cymene (16.2%, limonene (3.9%, α-terpinene (3.3%, myrcene (2.5%, germacrene B (1.4%, elemicine (1.1% and carvacrol (0.5%. The S. intermedia EO showed a concentration-dependent decrease in viability of Hep-G2 (hepatocellular carcinoma and MCF-7 (breast adenocarcinoma human cancer cell lines (p < 0.05. Antimicrobial screening of S. intermedia EO demonstrated slight antibacterial and antifungal activities against Streptococcus mutants, S. salivarius, Enterococcus faecalis, Staphylococcus aureus, Candida albicans and C. glabrata. Further preclinical studies are needed to assess the efficacy and safety of S. intermedia EO as a new promising anticancer agent.

  12. Identification and functional analysis of the gene cluster for fructan utilization in Prevotella intermedia.

    Science.gov (United States)

    Fuse, Haruka; Fukamachi, Haruka; Inoue, Mitsuko; Igarashi, Takeshi

    2013-02-25

    Fructanase enzymes hydrolyze the β-2,6 and β-2,1 linkages of levan and inulin fructans, respectively. We analyzed the influence of fructan on the growth of Prevotella intermedia. The growth of P. intermedia was enhanced by addition of inulin, implying that P. intermedia could also use inulin. Based on this finding, we identified and analyzed the genes encoding a putative fructanase (FruA), sugar transporter (FruB), and fructokinase (FruK) in the genome of strain ATCC25611. Transcript analysis by RT-PCR showed that the fruABK genes were co-transcribed as a single mRNA and semi-quantitative analysis confirmed that the fruA gene was induced in response to fructose and inulin. Recombinant FruA and FruK were purified and characterized biochemically. FruA strongly hydrolyzed inulin, with slight degradation of levan via an exo-type mechanism, revealing that FruA is an exo-β-d-fructanase. FruK converted fructose to fructose-6-phosphate in the presence of ATP, confirming that FruK is an ATP-dependent fructokinase. These results suggest that P. intermedia can utilize fructan as a carbon source for growth, and that the fructanase, sugar transporter, and fructokinase proteins we identified are involved in this fructan utilization. Copyright © 2012 Elsevier B.V. All rights reserved.

  13. Prevotella falsenii sp. nov., a Prevotella intermedia-like organism isolated from monkey dental plaque.

    Science.gov (United States)

    Sakamoto, Mitsuo; Kumada, Hidefumi; Hamada, Nobushiro; Takahashi, Yusuke; Okamoto, Masaaki; Bakir, Mohammad Abdul; Benno, Yoshimi

    2009-02-01

    Eight anaerobic, pigmented, non-spore-forming, Gram-negative, rod-shaped strains isolated from monkey oral cavities were characterized phenotypically and chemotaxonomically and their phylogenetic positions were determined using 16S rRNA gene sequence analysis. The 16S rRNA gene sequence analysis showed that these isolates represent a single species of the genus Prevotella. These strains were most closely related to Prevotella intermedia ATCC 25611(T), with 95.0 % 16S rRNA gene sequence similarity. The next most closely related species were Prevotella pallens and Prevotella nigrescens (92.7 and 92.1 % similarity to the respective type strains). The phenotypic and biochemical characteristics of the isolates were the same as those of P. intermedia JCM 12248(T) and P. nigrescens JCM 12250(T). The isolates could be differentiated from P. pallens JCM 11140(T) on the basis of mannose fermentation and alpha-fucosidase activity. The isolates could not be distinguished from P. intermedia or P. nigrescens using conventional biochemical tests. DNA-DNA hybridization experiments revealed the genomic distinctiveness of these eight strains with respect to P. pallens JCM 11140(T), P. intermedia JCM 12248(T) and P. nigrescens JCM 12250(T). On the basis of these data, strains 04013, 04021, 04043, 04052(T), 0406, 04113, 04111 and 04161 represent a novel Prevotella species, for which the name Prevotella falsenii sp. nov. is proposed. The type strain is 04052(T) (=JCM 15124(T) =CCUG 56137(T)).

  14. In vitro activation of the hemolysin in Prevotella nigrescens ATCC 33563 and Prevotella intermedia ATCC 25611.

    Science.gov (United States)

    Silva, Tarcília Aparecida; Noronha, Fátima Soares M; de Macêdo Farias, Luiz; Carvalho, Maria Auxiliadora R

    2004-01-01

    Hemolytic activity was evaluated in the putative periodontopathogens Prevotella intermedia and Prevotella nigrescens. Whole cells of both species present weak hemolytic activity evidenced only by solid media assays after 48 h of bacterial growth or after 5 h of interaction with erythrocytes at 37 degrees C in liquid assays. In this work we show that the use of crude extract allowed the detection of a higher hemolytic activity for P. intermedia, but surprisingly not for P. nigrescens. Incubation at 37 degrees C for 9 h, or treatment with trypsin or proteinase K, increased or exposed the hemolytic activity of P. intermedia and P. nigrescens crude extract, respectively. The activation process was inhibited by TLCK and PMSF but not by EDTA, E-64 or pepstatin A, indicating the serino-protease nature of the factor involved in activation of P. intermedia and P. nigrescens hemolysins. Both the buffer and the pH employed for cell fractionation influenced the activation of hemolysin, and the best results were obtained with Universal buffer at pH 8.0. The activated hemolysins acted optimally at pH 6.5 at 37 degrees C and the maximum hemolytic activity was detected at the early log phase of growth. The results of this study show for the first time a strong hemolytic activity for P. nigrescens and evidence of proteolytic activation of hemolysins produced by periodontopathogens.

  15. Antimicrobial Susceptibilities of Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens spp. Isolated in Spain

    OpenAIRE

    Andrés, María T.; Chung, Whasun O.; Roberts, Marilyn C.; Fierro, José F.

    1998-01-01

    The susceptibilities of 143 Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens isolates to 18 antimicrobial agents were tested. All P. gingivalis isolates were susceptible. In contrast, some Prevotella spp. (17%) were resistant to β-lactams, erythromycin, clindamycin, or tetracycline and carried resistance genes, ermF or tetQ, or β-lactamases.

  16. Antimicrobial Susceptibilities of Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens spp. Isolated in Spain

    Science.gov (United States)

    Andrés, María T.; Chung, Whasun O.; Roberts, Marilyn C.; Fierro, José F.

    1998-01-01

    The susceptibilities of 143 Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens isolates to 18 antimicrobial agents were tested. All P. gingivalis isolates were susceptible. In contrast, some Prevotella spp. (17%) were resistant to β-lactams, erythromycin, clindamycin, or tetracycline and carried resistance genes, ermF or tetQ, or β-lactamases. PMID:9797247

  17. Isolation of Yersinia enterocolitica and Y. intermedia from fatal cases of diarrhoeal illness in Bangladesh

    NARCIS (Netherlands)

    Butler, T.; Islam, M.; Islam, M. R.; Azad, A. K.; Huq, M. I.; Speelman, P.; Roy, S. K.

    1984-01-01

    From three fatal cases of diarrhoeal illness in Bangladesh, Yersinia species were isolated from tissues at post-mortem examination. One patient was infected with Y. enterocolitica serotype 0:7, 8 and two patients were infected with Y. intermedia. These patients were infected also with other enteric

  18. The contingency of intermedia agenda setting: a longitudinal study in Belgium

    NARCIS (Netherlands)

    Vliegenthart, R.; Walgrave, S.

    2008-01-01

    This large-scale study investigates how intermedia agenda-setting effects are moderated by five factors: (1) lag length; (2) medium type; (3) language/institutional barriers; (4) issue type; and (5) election or non-election context. Longitudinal analyses of daily attention to twenty-five issues in

  19. Experimental weed control of Najas marina ssp. intermedia and Elodea nuttallii in lakes using biodegradable jute matting

    Directory of Open Access Journals (Sweden)

    Markus A. Hoffmann

    2013-10-01

    Full Text Available The use of jute matting in managing the invasive aquatic macrophyte species Elodea nuttallii (Planch. H. St. John and Najas marina ssp. intermedia (Wolfg. ex Gorski Casper (Najas intermedia was studied in laboratory experiments and field trials. Four German lakes with predominant population of Najas intermedia or Elodea nuttalli were chosen for the experiment and areas between 150 and 300 m² were covered with jute textile. The effect of the matting on the growth of invasive and non-invasive macrophytes was determined through comparison with control transects. Biodegradable jute matting successfully suppressed the invasive macrophyte Najas intermedia and significantly reduced the growth of Elodea nuttalli in lakes. The results indicate that the capability of the matting to inhibit the growth of Elodea nuttallii and Najas intermedia depends on the mesh size of the jute weaving and that environmental conditions can affect its efficiency. Various indigenous species like Charales or Potamogeton pusillus L. were able to grow through the jute fabric and populate the treated areas. Until the end of the vegetation period, none of the invasive species were able to penetrate the covering and establish a stable population; in fact, in the subsequent year the jute matting affected only the spread of Najas intermedia. Jute matting proved to be an easy-to-use and cheap method to control the growth of Elodea nuttallii and Najas intermedia.

  20. Taxonomic study on Japanese Salvia (Lamiaceae): Phylogenetic position of S. akiensis, and polyphyletic nature of S. lutescens var. intermedia.

    Science.gov (United States)

    Takano, Atsuko

    2017-01-01

    Both Salvia akiensis and S. lutescens (Lamiaceae) are endemic to Japan. Salvia akiensis was recently described in 2014 in the Chugoku (= SW Honshu) region, and each four varieties of S. lutescens distributed allopatrically. Among varieties in S. lutescens , var. intermedia show a disjunctive distribution in the Kanto (=E Honshu) and Kinki (= W Honshu) regions. Recent field studies of S. lutescens var. intermedia revealed several morphological differences between the Kanto and Kinki populations. Here, I evaluated these differences among Salvia lutescens var. intermedia and its allies with morphological analysis and molecular phylogenetic analyses of nuclear ribosomal DNA (internal and external transcribed spacer regions) and plastid DNA ( ycf1-rps15 spacer, rbcL , and trnL-F ) sequences. Both morphological analysis and molecular phylogenetic analyses showed that S. lutescens var. intermedia from the Kinki region and var. lutescens were closely related to each other. However, var. intermedia from the Kanto region exhibited an association with S. lutescens var. crenata and var. stolonifera, which also grew in eastern Japan, rather than var. intermedia in the Kinki region. These results indicated that S. lutescens var. intermedia is not a taxon with a disjunctive distribution, but a combination of two or more allopatric taxa. Present study also suggested that S. akiensis was most closely related to S. omerocalyx .

  1. Development of Prevotella intermedia-specific PCR primers based on the nucleotide sequences of a DNA probe Pig27.

    Science.gov (United States)

    Kim, Min Jung; Hwang, Kyung Hwan; Lee, Young-Seok; Park, Jae-Yoon; Kook, Joong-Ki

    2011-03-01

    The aim of this study was to develop Prevotella intermedia-specific PCR primers based on the P. intermedia-specific DNA probe. The P. intermedia-specific DNA probe was screened by inverted dot blot hybridization and confirmed by Southern blot hybridization. The nucleotide sequences of the species-specific DNA probes were determined using a chain termination method. Southern blot analysis showed that the DNA probe, Pig27, detected only the genomic DNA of P. intermedia strains. PCR showed that the PCR primers, Pin-F1/Pin-R1, had species-specificity for P. intermedia. The detection limits of the PCR primer sets were 0.4pg of the purified genomic DNA of P. intermedia ATCC 49046. These results suggest that the PCR primers, Pin-F1/Pin-R1, could be useful in the detection of P. intermedia as well as in the development of a PCR kit in epidemiological studies related to periodontal diseases. Crown Copyright © 2010. Published by Elsevier B.V. All rights reserved.

  2. Comparison of the virulence of exopolysaccharide-producing Prevotella intermedia to exopolysaccharide non-producing periodontopathic organisms

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    Walker Clay B

    2011-08-01

    Full Text Available Abstract Background Evidence in the literature suggests that exopolysaccharides (EPS produced by bacterial cells are essential for the expression of virulence in these organisms. Secreted EPSs form the framework in which microbial biofilms are built. Methods This study evaluates the role of EPS in Prevotella intermedia for the expression of virulence. This evaluation was accomplished by comparing EPS-producing P. intermedia strains 17 and OD1-16 with non-producing P. intermedia ATCC 25611 and Porphyromonas gingivalis strains ATCC 33277, 381 and W83 for their ability to induce abscess formation in mice and evade phagocytosis. Results EPS-producing P. intermedia strains 17 and OD1-16 induced highly noticeable abscess lesions in mice at 107 colony-forming units (CFU. In comparison, P. intermedia ATCC 25611 and P. gingivalis ATCC 33277, 381 and W83, which all lacked the ability to produce viscous materials, required 100-fold more bacteria (109 CFU in order to induce detectable abscess lesions in mice. Regarding antiphagocytic activity, P. intermedia strains 17 and OD1-16 were rarely internalized by human polymorphonuclear leukocytes, but other strains were readily engulfed and detected in the phagosomes of these phagocytes. Conclusions These results demonstrate that the production of EPS by P. intermedia strains 17 and OD1-16 could contribute to the pathogenicity of this organism by conferring their ability to evade the host's innate defence response.

  3. Comparison of the virulence of exopolysaccharide-producing Prevotella intermedia to exopolysaccharide non-producing periodontopathic organisms.

    Science.gov (United States)

    Yamanaka, Takeshi; Yamane, Kazuyoshi; Furukawa, Tomoyo; Matsumoto-Mashimo, Chiho; Sugimori, Chieko; Nambu, Takayuki; Obata, Noboru; Walker, Clay B; Leung, Kai-Poon; Fukushima, Hisanori

    2011-08-25

    Evidence in the literature suggests that exopolysaccharides (EPS) produced by bacterial cells are essential for the expression of virulence in these organisms. Secreted EPSs form the framework in which microbial biofilms are built. This study evaluates the role of EPS in Prevotella intermedia for the expression of virulence. This evaluation was accomplished by comparing EPS-producing P. intermedia strains 17 and OD1-16 with non-producing P. intermedia ATCC 25611 and Porphyromonas gingivalis strains ATCC 33277, 381 and W83 for their ability to induce abscess formation in mice and evade phagocytosis. EPS-producing P. intermedia strains 17 and OD1-16 induced highly noticeable abscess lesions in mice at 107 colony-forming units (CFU). In comparison, P. intermedia ATCC 25611 and P. gingivalis ATCC 33277, 381 and W83, which all lacked the ability to produce viscous materials, required 100-fold more bacteria (109 CFU) in order to induce detectable abscess lesions in mice. Regarding antiphagocytic activity, P. intermedia strains 17 and OD1-16 were rarely internalized by human polymorphonuclear leukocytes, but other strains were readily engulfed and detected in the phagosomes of these phagocytes. These results demonstrate that the production of EPS by P. intermedia strains 17 and OD1-16 could contribute to the pathogenicity of this organism by conferring their ability to evade the host's innate defence response.

  4. Relative contribution of Prevotella intermedia and Pseudomonas aeruginosa to lung pathology in airways of patients with cystic fibrosis.

    Science.gov (United States)

    Ulrich, Martina; Beer, Isabelle; Braitmaier, Peter; Dierkes, Michaela; Kummer, Florian; Krismer, Bernhard; Schumacher, Ulrike; Gräpler-Mainka, Ute; Riethmüller, Joachim; Jensen, Peter Ø; Bjarnsholt, Thomas; Høiby, Niels; Bellon, Gabriel; Döring, Gerd

    2010-11-01

    Patients with cystic fibrosis (CF) with Pseudomonas aeruginosa lung infections produce endobronchial mucus plugs allowing growth of obligate anaerobes including Prevotella spp. Whether obligate anaerobes contribute to the pathophysiology of CF lung disease is unknown. The virulence of Prevotella intermedia and Ps aeruginosa was investigated in vitro and in mice, antibodies against P intermedia in CF sera were assessed and a culture-independent detection method for P intermedia/P nigrescens in CF sputum was tested. P intermedia reached cell numbers of >10(5)->10(7) colony-forming units (CFU)/ml sputum. The majority of patients with CF (16/17; 94.1%) produced antibodies against two immunoreactive antigens of P intermedia. Culture supernatant fluids, collected from 10(9) P intermedia cells, were more cytotoxic to respiratory epithelial cells in vitro and inflammatory in mouse lungs than respective fluids from anaerobically grown Ps aeruginosa, while fluids from aerobically grown Ps aeruginosa had the highest cytotoxicity and inflammation. Both pathological effects were largely reduced when culture supernatant fluids from 10(7) cells of either species were used. P intermedia cells (∼10(6)CFU/lung) did not induce mortality in the agar beads lung infection mouse model, while Ps aeruginosa cells caused death in 30% of mice due to rapid multiplication. A P intermedia/P nigrescens-specific PNA probe was significantly more sensitive than culture-dependent diagnostic assays to detect these strict anaerobes. Ps aeruginosa and P intermedia become significantly virulent in vitro and in vivo when cell numbers exceed 10(8) CFU/lung.

  5. Ablación de taquicardia supraventricular resistente a terapia médica y a cardioversión eléctrica en una gestante

    Directory of Open Access Journals (Sweden)

    Edison Muñoz Ortiz

    2018-01-01

    Full Text Available Las arritmias cardiacas son complicaciones frecuentes durante el embarazo, y de hecho se ha considerado que este estado incrementa la incidencia de arritmias en pacientes con y sin enfermedad cardiaca estructural. El manejo de las arritmias en la paciente embarazada debe tener en cuenta no solo el bienestar materno sino el fetal, característica que restringe muchas de las terapias usadas en población no embarazada; sin embargo, es poco frecuente la falta de respuesta a terapia médica y cardioversión eléctrica. Se presenta el caso de una paciente gestante con taquicardia supraventricular por reentrada por vía accesoria sin respuesta a diferentes medidas farmacológicas y a varios intentos de cardioversión eléctrica, que requirió estudio electrofisiológico y ablación emergentes. Adicionalmente, se listan algunos aspectos claves sobre el tema.

  6. La alimentación como terapia natural: la granada en el tratamiento de cáncer de próstata

    OpenAIRE

    Ruiz Aja, Elba

    2013-01-01

    El cáncer de próstata es uno de los tumores con mayor incidencia entre los hombres, siendo la tercera causa de fallecimiento por cáncer en el sexo masculino en España, detrás del cáncer de pulmón y del cáncer colorrectal. Debido a esto, es importante encontrar nuevas terapias que ayuden a detener e incluso reducir su progresión. La quimioprevención, que es el uso de ciertos alimentos para retrasar el proceso carcinogénico, ha demostrado ser útil como terapia alternativa en múltiples estudios,...

  7. Effect of azithromycin on Prevotella intermedia lipopolysaccharide-induced production of interleukin-6 in murine macrophages.

    Science.gov (United States)

    Choi, Eun-Young; Jin, Ji-Young; Choi, Jeom-Il; Choi, In Soon; Kim, Sung-Jo

    2014-04-15

    Interleukin-6 (IL-6) is a key proinflammatory cytokine which plays a central role in the pathogenesis of periodontal disease. Host modulatory agents targeting at inhibiting IL-6, therefore, appear to be beneficial in slowing the progression of periodontal disease and potentially reducing destructive aspects of the host response. The present study was designed to investigate the effect of the macrolide antibiotic azithromycin on IL-6 generation in murine macrophages treated with lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen implicated in inflammatory periodontal disease, and its mechanisms of action. Azithromycin significantly suppressed IL-6 production as well as its mRNA expression in P. intermedia LPS-activated RAW264.7 cells. LPS-induced activation of JNK and p38 was not affected by azithromycin treatment. Azithromycin failed to prevent P. intermedia LPS from degrading IκB-α. Instead, azithromycin significantly diminished nuclear translocation and DNA binding activity of NF-κB p50 subunit induced with LPS. Azithromycin inhibited P. intermedia LPS-induced STAT1 and STAT3 phosphorylation. In addition, azithromycin up-regulated the mRNA level of SOCS1 in cells treated with LPS. In conclusion, azithromycin significantly attenuated P. intermedia LPS-induced production of IL-6 in murine macrophages via inhibition of NF-κB, STAT1 and STAT3 activation, which is possibly related to the activation of SOCS1 signaling. Further in vivo studies are required to better evaluate the potential of azithromycin in the treatment of periodontal disease. Copyright © 2014 Elsevier B.V. All rights reserved.

  8. Inhibitory effects of lactoferrin on growth and biofilm formation of Porphyromonas gingivalis and Prevotella intermedia.

    Science.gov (United States)

    Wakabayashi, Hiroyuki; Yamauchi, Koji; Kobayashi, Tetsuo; Yaeshima, Tomoko; Iwatsuki, Keiji; Yoshie, Hiromasa

    2009-08-01

    Lactoferrin (LF) is an iron-binding antimicrobial protein present in saliva and gingival crevicular fluids, and it is possibly associated with host defense against oral pathogens, including periodontopathic bacteria. In the present study, we evaluated the in vitro effects of LF-related agents on the growth and biofilm formation of two periodontopathic bacteria, Porphyromonas gingivalis and Prevotella intermedia, which reside as biofilms in the subgingival plaque. The planktonic growth of P. gingivalis and P. intermedia was suppressed for up to 5 h by incubation with >or=130 microg/ml of human LF (hLF), iron-free and iron-saturated bovine LF (apo-bLF and holo-bLF, respectively), and >or=6 microg/ml of bLF-derived antimicrobial peptide lactoferricin B (LFcin B); but those effects were weak after 8 h. The biofilm formation of P. gingivalis and P. intermedia over 24 h was effectively inhibited by lower concentrations (>or=8 microg/ml) of various iron-bound forms (the apo, native, and holo forms) of bLF and hLF but not LFcin B. A preformed biofilm of P. gingivalis and P. intermedia was also reduced by incubation with various iron-bound bLFs, hLF, and LFcin B for 5 h. In an examination of the effectiveness of native bLF when it was used in combination with four antibiotics, it was found that treatment with ciprofloxacin, clarithromycin, and minocycline in combination with native bLF for 24 h reduced the amount of a preformed biofilm of P. gingivalis compared with the level of reduction achieved with each agent alone. These results demonstrate the antibiofilm activity of LF with lower iron dependency against P. gingivalis and P. intermedia and the potential usefulness of LF for the prevention and treatment of periodontal diseases and as adjunct therapy for periodontal diseases.

  9. Contribution of hly homologs to the hemolytic activity of Prevotella intermedia.

    Science.gov (United States)

    Suzuki, Naoko; Fukamachi, Haruka; Arimoto, Takafumi; Yamamoto, Matsuo; Igarashi, Takeshi

    2012-06-01

    Prevotella intermedia is a periodontal pathogen that requires iron for its growth. Although this organism has hemolytic activity, the precise nature of its hemolytic substances and their associated hemolytic actions are yet to be fully determined. In the present study, we identified and characterized several putative hly genes in P. intermedia ATCC25611 which appear to encode hemolysins. Six hly genes (hlyA, B, C, D, E, and hlyI) of P. intermedia were identified by comparing their nucleotide sequences to those of known hly genes of Bacteroides fragilis NCTC9343. The hlyA-E, and hlyI genes were overexpressed individually in the non-hemolytic Escherichia coli strain JW5181 and examined its contribution to the hemolytic activity on sheep blood agar plates. E. coli cells expressing the hlyA and hlyI genes exhibited hemolytic activity under anaerobic conditions. On the other hand, only E. coli cells stably expressing the hlyA gene were able to lyse the red blood cells when cultured under aerobic conditions. In addition, expression of the hlyA and hlyI genes was significantly upregulated in the presence of red blood cells. Furthermore, we found that the growth of P. intermedia was similar in an iron-limited medium supplemented with either red blood cells or heme. Taken together, our results indicate that the hlyA and hlyI genes of P. intermedia encode putative hemolysins that appear to be involved in the lysis of red blood cells, and suggest that these hemolysins might play important roles in the iron-dependent growth of this organism. Copyright © 2012 Elsevier Ltd. All rights reserved.

  10. Functional characterization and epitope analysis of a recombinant dermonecrotic protein from Loxosceles intermedia spider.

    Science.gov (United States)

    Felicori, L; Araujo, S C; de Avila, R A Machado; Sanchez, E F; Granier, C; Kalapothakis, E; Chávez-Olórtegui, C

    2006-10-01

    In the present study the recombinant form (recLiD1) of a dermonecrotic protein present in the Brazilian brown spider Loxosceles intermedia venom was expressed in Escherichia coli cells and purified by reversed-phase HPLC using a C8 Vydac column. About 25.8mg of purified recLiD1 was produced from a litre of bacterial culture. SDS/PAGE and immunoblot analysis of the recombinant protein revealed an apparent molecular weight of 32-35kDa. The later result was confirmed by mass spectrometry (32,758Da). recLiD1 displayed dermonecrotic and platelet aggregation activities which were qualitatively similar to that displayed by the crude L. intermedia venom. However, very low sphingomyelinase D enzymatic activity and complement-dependent haemolytic activities were observed. recLiD1 immunized BALB/c mice developed an antibody response. Anti-recLiD1 antibodies recognized L. intermedia venom in an indirect enzyme-linked immunosorbent assay (ELISA) and cross-reacted with crude venoms from L. intermedia, L. gaucho and L. laeta. An in vivo protection assay carried out 5 weeks after the end of the immunization protocol showed that 75% of the vaccinated mice could resist the challenge by 2.5LD(50) of L. intermedia venom. To characterize epitopes associated with protective antibodies, we prepare sets of immobilized synthetic 15 mer overlapping peptides covering the complete amino acid sequences of the recLiD1. Antibodies revealed one antigenic region in the N-terminal part of the toxin. The amino acid sequence of this epitope was found in several dermonecrotic proteins and some of its residues have been implicated with the active site of the toxin.

  11. Sendo enfermeira no centro de terapia intensiva

    Directory of Open Access Journals (Sweden)

    Adrina Katia Corrêa

    1995-09-01

    Full Text Available Encaminhando-me na perspectiva da fenomenologia, minha proposta é apreender, através dos depoimentos das enfermeiras que trabalham no CTI, os significados que as mesmas atribuem a essa vivência. Ao refletir sobre tais significados, aliando-os à minha experiência profissional, busco compreender o fenômeno ser enfermeira no CTI. Coletei depoimentos escritos das enfermeiras que atuavam no centro de terapia intensiva da Unidade de Emergência do Hospital das Clínicas da Faculdade de Medicina - Ribeirão Preto - USP, tendo como questão orientadora: o que significa para você ser enfermeira no CTI. Da análise desses depoimentos (MARTINS, 1992, emergiram as categorias temáticas, revelando que ser enfermeira no CTI é cuidar diretamente dos pacientes, é ter capacidade administrativa, é conviver com máquinas e equipamentos, exige muitos requisitos e o domínio de conhecimentos específicos, é desempenhar um trabalho importante, de responsabilidade e gratificante, bem como, conviver com situações limitantes e de estresse. A partir de uma reflexão compreensiva do modo de ser da enfermeira desse CTI, pretendo contribuir para busca de novas perspectivas do fazer, ampliando seu horizonte de possibilidades.

  12. Terapia familiar en los trastornos de personalidad

    Directory of Open Access Journals (Sweden)

    Laura Rodríguez Moya

    2013-06-01

    Full Text Available La enfermedad mental afecta no solo al paciente sino también a su familia, dificulta la comunicación intrafamiliar, puede generar patologías adicionales en los familiares y todo ello empeorar la evolución del trastorno. Las terapias familiares de tipo psicoeducativo mejoran el pronóstico de la enfermedad y además previenen la aparición de otros trastornos en los familiares. Los elementos más importantes de estas intervenciones son la psicoeducación sobre el trastorno, el re-establecimiento de una relación sana entre paciente-familia, el establecimiento de límites, la mejora de la comunicación familiar y el establecimiento de relaciones con la red social extensa, para evitar el aislamiento tanto de la familia como del paciente. Las intervenciones mixtas, individuales y familiares, han demostrado ser muy útiles en el caso de los trastornos de personalidad, en concreto en el Trastorno Límite de Personalidad (TLP.

  13. Percepções de profissionais de enfermagem sobre intervenções de Terapia Ocupacional em Saúde Mental em hospital universitário Percepciones de profesionales de enfermería sobre intervenciones de Terapia Ocupacional en Salud Mental en hospital universitario Perceptions of professional nurses about occupational therapy interventions in mental health at a university hospital

    Directory of Open Access Journals (Sweden)

    Solange Tedesco

    2011-01-01

    Full Text Available OBJETIVO: Conhecer as percepções de profissionais de enfermagem sobre intervenções grupais de Terapia Ocupacional em Saúde Mental realizadas com pacientes internados em um hospital universitário. MÉTODOS: Estudo de abordagem qualitativa do tipo descrito. As anotações das coordenadoras dos grupos de Terapia Ocupacional foram analisadas em relação às entrevistas abertas junto a n enfermeiras e n técnicos de enfermagem que participaram das intervenções grupais de Terapia Ocupacional pelo período de dois anos. As respostas foram submetidas à análise do Discurso do Sujeito Coletivo (DSC. RESULTADOS: Foram identificadas quatro ideias centrais do DSC: a Terapia Ocupacional como favorecedora do cuidado integrado, valorizando o sujeito e sua experiência; a terapia ocupacional como processo de ajuda e cuidado para a própria equipe; o grupo de Terapia Ocupacional como espaço de ressonância e facilitação no manejo com o paciente em razão da percepção de aspectos relacionais. CONCLUSÃO: As estratégias foram percebidas, quer como promotoras da reorganização da situação vivida pelo paciente na internação, quer como oportunidades de ensino e apoio para a equipe de enfermagem.OBJETIVO: Conocer las percepciones de profesionales de enfermería sobre intervenciones grupales de Terapia Ocupacional en Salud Mental realizadas con pacientes internados en un hospital universitario. MÉTODOS: Estudio de abordaje cualitativo del tipo descrito. Las anotaciones de las coordinadoras de los grupos de Terapia Ocupacional fueron analizadas en relación a las entrevistas abiertas junto a n enfermeras y n técnicos de enfermería que participaron de las intervenciones grupales de Terapia Ocupacional por el período de dos años. Las respuestas fueron sometidas al análisis de Discurso del Sujeto Colectivo (DSC. RESULTADOS: Se identificaron cuatro ideas centrales del DSC: la Terapia Ocupacional como favorecedora del cuidado integrado, valorizando

  14. ASSISTÊNCIA AO PACIENTE EM PARADA CARDIORRESPIRATÓRIA EM UNIDADE DE TERAPIA INTENSIVA

    Directory of Open Access Journals (Sweden)

    Luiza Taciana Rodrigues de Moura

    2012-01-01

    Full Text Available Atención a la parada cardiorrespiratoria debe ser sistemática, basada en los protocolos de soporte básico y avanzado de vida. El objetivo fue evaluar el conocimiento del personal del equipo de enfermeira de unidad de terapia intensiva en relación al reconocimiento de la parada cardiorrespiratoria y establecimiento de la reanimación de acuerdo con los protocolos. Estudio descriptivo y cuantitativo, llevado a cabo entre abril y junio de 2011. De los 33 profesionales que participaron del estudio , 54,5% no habían recibido formación previa sobre el tema, 93,9% acertaron parcialmente los ritmos de parada, 15,2 % acertaron totalmente las maniobras de ventilación en pacientes intubados. El bajo índice de aciertos totales señaló la necesidad de actualización del equipo de enfermería, con capacitación teórica y práctica periódicamente y evaluación sistemática del desempeño del equipo.

  15. El Uso terapéutico del self como herramienta en Terapia Ocupacional

    OpenAIRE

    Pérez Corrales, Jorge

    2014-01-01

    V Jornadas de actualización en terapia ocupacional: Formación continuada en terapia ocupacional, celebradas en la Universidad Rey Juan Carlos campus de Alcorcón en 2014. Fisioterapia, Terapia Ocupacional, Rehabilitación y Medicina Física

  16. Terapias creativas en la unidad de hospitalización breve psiquiátrica

    OpenAIRE

    Asensio, Carmen

    2014-01-01

    V Jornadas de actualización en terapia ocupacional: Formación continuada en terapia ocupacional, celebradas en la Universidad Rey Juan Carlos campus de Alcorcón en 2014. Fisioterapia, Terapia Ocupacional, Rehabilitación y Medicina Física

  17. Comparative genome analysis of Prevotella intermedia strain isolated from infected root canal reveals features related to pathogenicity and adaptation.

    Science.gov (United States)

    Ruan, Yunfeng; Shen, Lu; Zou, Yan; Qi, Zhengnan; Yin, Jun; Jiang, Jie; Guo, Liang; He, Lin; Chen, Zijiang; Tang, Zisheng; Qin, Shengying

    2015-02-25

    Many species of the genus Prevotella are pathogens that cause oral diseases. Prevotella intermedia is known to cause various oral disorders e.g. periodontal disease, periapical periodontitis and noma as well as colonize in the respiratory tract and be associated with cystic fibrosis and chronic bronchitis. It is of clinical significance to identify the main drive of its various adaptation and pathogenicity. In order to explore the intra-species genetic differences among strains of Prevotella intermedia of different niches, we isolated a strain Prevotella intermedia ZT from the infected root canal of a Chinese patient with periapical periodontitis and gained a draft genome sequence. We annotated the genome and compared it with the genomes of other taxa in the genus Prevotella. The raw data set, consisting of approximately 65X-coverage reads, was trimmed and assembled into contigs from which 2165 ORFs were predicted. The comparison of the Prevotella intermedia ZT genome sequence with the published genome sequence of Prevotella intermedia 17 and Prevotella intermedia ATCC25611 revealed that ~14% of the genes were strain-specific. The Preveotella intermedia strains share a set of conserved genes contributing to its adaptation and pathogenic and possess strain-specific genes especially those involved in adhesion and secreting bacteriocin. The Prevotella intermedia ZT shares similar gene content with other taxa of genus Prevotella. The genomes of the genus Prevotella is highly dynamic with relative conserved parts: on average, about half of the genes in one Prevotella genome were not included in another genome of the different Prevotella species. The degree of conservation varied with different pathways: the ability of amino acid biosynthesis varied greatly with species but the pathway of cell wall components biosynthesis were nearly constant. Phylogenetic tree shows that the taxa from different niches are scarcely distributed among clades. Prevotella intermedia ZT

  18. Terapia nutricional em Unidade de Terapia Intensiva Nutritional therapy in Intensive Care Unit

    Directory of Open Access Journals (Sweden)

    Iára Kallyanna Cavalcante Ferreira

    2007-03-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: Esta revisão tem como objetivo levantar os principais aspectos necessários para a realização de terapia nutricional segura e eficaz ao paciente crítico. CONTEÚDO: Foi feito um levantamento bibliográfico com livros didáticos e artigos científicos em Português, Inglês e Espanhol com resultados dos últimos 20 anos.A terapia nutricional é parte integrante dos cuidados do paciente em unidades de terapia intensiva. O seu sucesso envolve as etapas de avaliação nutricional, determinação das necessidades de calorias e nutrientes, decisão da via de infusão e o tipo de dieta empregada. CONCLUSÕES: O uso de nutrientes com a finalidade de melhorar a função imunológica (imunonutrientes, é cada vez mais freqüente, porém seu uso não está bem estabelecido para pacientes críticos. Mais estudos clínicos são necessários para estabelecer a melhor forma de nutrir o paciente crítico.BACKGROUND AND OBJECTIVES: The purpose of this review is to approach the main necessary aspects for the accomplishment of safety and efficient nutritional therapy to the critically ill patient. CONTENTS: Bibliographical survey with didactic books and scientific articles was made in Portuguese, English and Spanish with results of the last 20 years. Nutritional support is an integrant part in the care of patients in intensive care units. The success of the nutritional therapy involves the stages of nutritional assessment, determines the route of diet infusion and the calories and nutrients needs. CONCLUSIONS: The use of nutrients with immune function (immunonutrients is each more frequents, however, its use is not well established for critical illness. More clinical studies are necessary to establish the best form to nourish the critical ill patient.

  19. As terapias alternativas na assistência de enfermagem

    Directory of Open Access Journals (Sweden)

    Jackçon L. Savi

    1995-12-01

    Full Text Available Na seqüência do acompanhamento da disciplina "Métodos Terapêuticos Alternativos" (MTA. implantada no Curso de Graduação em Enfermagem da UFSC em 1990. focalizou-se os egressos com o objetivo de verificar a utilização das terapias alternativas na assistência de enfermagem que prestam como enfermeiros. Nenhum dos entrevistados posicionou-se contrário e a maioria confirmou utilizar uma ou mais terapias na assistência que prestam aos usuários dos serviços onde atuam.

  20. Terapia cognitivo-comportamental da depressão

    OpenAIRE

    Powell,Vania Bitencourt; Abreu,Neander; Oliveira,Irismar Reis de; Sudak,Donna

    2008-01-01

    OBJETIVO: Descrever o uso de técnicas cognitivas e revisar os estudos de eficácia da terapia cognitivo-comportamental no tratamento da depressão. MÉTODO: Revisão não-sistemática proveniente dos estudos originais, complementada por informações provenientes de metanálises e livros-texto especializados. RESULTADOS: Foram descritos os fundamentos da terapia cognitivo-comportamental no tratamento da depressão e revisadas as evidências de eficácia em curto e longo prazo. Discutimos igualmente o uso...

  1. Terapia cognitivo-comportamental da depressão

    OpenAIRE

    Powell, Vania Bitencourt; Abreu, Neander; Oliveira, Irismar Reis de; Sudak, Donna

    2008-01-01

    P. 73-80. OBJETIVO: Descrever o uso de técnicas cognitivas e revisar os estudos de eficácia da terapia cognitivo-comportamental no tratamento da depressão. MÉTODO: Revisão não-sistemática proveniente dos estudos originais, complementada por informações provenientes de metanálises e livros-texto especializados. RESULTADOS: Foram descritos os fundamentos da terapia cognitivo-comportamental no tratamento da depressão e revisadas as evidências de eficácia em curto e longo prazo. Discutim...

  2. As terapias alternativas na assistência de enfermagem

    Directory of Open Access Journals (Sweden)

    Jackçon L. Savi

    Full Text Available Na seqüência do acompanhamento da disciplina "Métodos Terapêuticos Alternativos" (MTA. implantada no Curso de Graduação em Enfermagem da UFSC em 1990. focalizou-se os egressos com o objetivo de verificar a utilização das terapias alternativas na assistência de enfermagem que prestam como enfermeiros. Nenhum dos entrevistados posicionou-se contrário e a maioria confirmou utilizar uma ou mais terapias na assistência que prestam aos usuários dos serviços onde atuam.

  3. Complicaciones cutáneas de la terapia inmunosupresora: estudio en trasplantados renales

    Directory of Open Access Journals (Sweden)

    Diego E. Jaramillo

    1988-01-01

    Full Text Available Se presenta la experiencia dermatológica obtenida durante el seguimiento prospectivo de 114 pacientes con trasplante renal, atendidos en el Hospital Universitario San Vicente de Paúl, de Medellín; su evolución post-trasplante fluctuaba entre 2 meses y 13 anos y el seguimiento dermatológico fue por dos anos. Se encontró un número elevado de manifestaciones cutáneas (promedio 4,8 por paciente; las más frecuentes fueron: acné, micosis, hirsutismo y xerosis; no se hallaron lesiones malignas. No hubo asociación de las manifestaciones cutáneas con la edad del paciente, el tipo de donante o el esquema Inmunosupresor. Dada la alta frecuencia de patología cutánea se sugiere que estos pacientes sean educados adecuadamente sobre este aspecto y examinados periódicamente con el fin de detectar y manejar a tiempo sus problemas dermatológicos benignos O potencialmente malignos.

  4. Complicaciones cutáneas de la terapia inmunosupresora: estudio en trasplantados renales

    OpenAIRE

    Diego E. Jaramillo; Beatriz Orozco

    1988-01-01

    Se presenta la experiencia dermatológica obtenida durante el seguimiento prospectivo de 114 pacientes con trasplante renal, atendidos en el Hospital Universitario San Vicente de Paúl, de Medellín; su evolución post-trasplante fluctuaba entre 2 meses y 13 anos y el seguimiento dermatológico fue por dos anos. Se encontró un número elevado de manifestaciones cutáneas (promedio 4,8 por paciente); las más frecuentes fueron: acné, micosis, hirsutismo y xerosis; no se hallaron lesiones malignas. No ...

  5. Leptin potentiates Prevotella intermedia lipopolysaccharide-induced production of TNF-alpha in monocyte-derived macrophages.

    Science.gov (United States)

    Kim, Sung-Jo

    2010-06-01

    In addition to regulating body weight, leptin is also recognized for its role in the regulation of immune function and inflammation. The purpose of this study was to investigate the effect of leptin on Prevotella (P.) intermedia lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-alpha production in differentiated THP-1 cells, a human monocytic cell line. LPS from P. intermedia ATCC 25611 was prepared by the standard hot phenol-water method. THP-1 cells were incubated in the medium supplemented with phorbol myristate acetate to induce differentiation into macrophage-like cells. The amount of TNF-alpha and interleukin-8 secreted into the culture medium was determined by enzyme-linked immunosorbent assay (ELISA). TNF-alpha and Ob-R mRNA expression levels were determined by semi-quantitative reverse transcription-polymerase chain reaction analysis. Leptin enhanced P. intermedia LPS-induced TNF-alpha production in a dose-dependent manner. Leptin modulated P. intermedia LPS-induced TNF-alpha expression predominantly at the transcriptional level. Effect of leptin on P. intermedia LPS-induced TNF-alpha production was not mediated by the leptin receptor. The ability of leptin to enhance P. intermedia LPS-induced TNF-alpha production may be important in the establishment of chronic lesion accompanied by osseous tissue destruction observed in inflammatory periodontal disease.

  6. Pi30 DNA probe may be useful for the identification of Prevotella intermedia at the species or strain level.

    Science.gov (United States)

    Shin, Yong Kook; Jeong, Seung-U; Yoo, So Young; Kim, Mi-Kwang; Kim, Hwa-Sook; Kim, Byung-Ock; Kim, Do Kyung; Hwang, Ho-Keel; Kook, Joong-Ki

    2004-01-01

    Recently, we introduced a new method for the rapid screening of bacterial species-or subspecies-specific DNA probes, named the "inverted dot blot hybridization screening method." This method has subsequently been then applied to develop species-or strain-specific DNA probes for Prevotella intermedia and Prevotella nigrescens. In a previous study, the inverted dot blot hybridization data showed that a probe, Pi30, was specific for P. intermedia. In this study, the DNA probe Pi30 was evaluated by Southern blot analysis to determine if it could distinguish P. intermedia from P. nigrescens. The data showed that the probe Pi30 reacted with the genomic DNAs from the reference strains and clinical isolates of both P. intermedia and P. nigrescens, but the size of the signal bands was different. In addition, the probe Pi30 reacted with a 1.4 kbp fragment from the genomic DNAs digested with Pst I of the P. intermedia strains but not with any fragments of P. nigrescens strains. The result indicates that the probe Pi30 could be useful for the identification of P. intermedia by restriction fragment length polymorphism (RFLP) at the species or strain level.

  7. Mídia Literary Multimodal and Intermedia Literacy: Buiding a Reader/Scriptor of New Medias

    Directory of Open Access Journals (Sweden)

    Ana Luiza Ramazzina Ghirardi

    2017-10-01

    Full Text Available This paper describes and discusses a course for undergraduate language students (Portuguese, Portuguese-French, Portuguese-English, Portuguese-Spanish held at the Escola de Filosofia, Letras e Ciências Humanas/UNIFESP, at the second semestre of 2015. The goal of the course, entitled “Literary Adaptation and Multimodality” was to help students understand, through the analysis of literary adaptations to different media, the key concepts in multimodality and intermedia literacy. The teaching strategy adopted was that of inviting the student to become a author/scriptor of a muldimodal intermedia transposition, reconfiguring the text of written literature (source media into different media – film, comics, stop-motion (source media.

  8. Networked Intermedia Agenda Setting: The Geography of a Hyperlinked Scandinavian News Ecology

    DEFF Research Database (Denmark)

    Sjøvaag, Helle; Stavelin, Eirik; Karlsson, Michael

    How does agenda setting work within the hyperlinked Scandinavian news ecology? This paper investigates intermedia agenda setting within and between the local, regional, national and supra-national levels in Sweden, Denmark and Norway; analyses the center/periphery dimensions of hyperlink connecti......How does agenda setting work within the hyperlinked Scandinavian news ecology? This paper investigates intermedia agenda setting within and between the local, regional, national and supra-national levels in Sweden, Denmark and Norway; analyses the center/periphery dimensions of hyperlink...... connections, and evaluates the extent of centralization of hyperlinked news agendas in the three countries. As hyperlinks are the backbone of the Internet, studying hyperlinking facilitates analyses of the relative connectedness of local, regional, national and international news agendas. The analysis...

  9. Extramedullary hematopoiesis with spinal cord compression in a child with thalassemia intermedia.

    Science.gov (United States)

    Ileri, Talia; Azik, Fatih; Ertem, Mehmet; Uysal, Zumrut; Gozdasoglu, Sevgi

    2009-09-01

    Spinal cord compression due to extramedullary hematopoiesis is an extremely rare complication of thalassemia intermedia. No cases with this complication have been reported in the first decade of life, because masses of heterotropic marrow developed in patients as a result of continuous erythropoiesis. We report the 9-year-old patient suffering from thalassemia intermedia and presenting spinal cord compression. We also review the literature about treatment options, because there is no consensus about the optimal treatment of these patients. Our patient was successfully treated with radiation therapy followed by hydroxyurea. With this combination therapy, he had no recurrence during the 4-year follow-up period. Clinical awareness of this phenomenon with the early treatment is essential for optimizing the successful outcome.

  10. [Removal of oral Prevotella intermedia Endotoxin by octyl phenyl polyoxyethylene ether extraction method].

    Science.gov (United States)

    Wang, Ai-wu; Liu, Yan; Hu, Kong-xin; Cheng, Qian

    2012-12-01

    To investigate an effective purification method for removing endotoxin from Prevotella intermedia. The main protein ingredients of bacteria prepared from ammonium sulfate precipitation were further treated with octyl phenyl polyoxyethylene ether (Triton X-114), and then processed at 4°C, 37°C and 25°C. The obtained aqueous phase after at least two more cycle repeated operations was assayed for endotoxin by Western blotting, LAL-clotting method, in vitro cell stimulation and in vivo animal experiments. Western blotting and LAL-clotting method demonstrated that the reduction in endotoxin level was greater than 99.99% and recovery of the proteins after endotoxin removal was greater than 90% with Triton X-114 treatment for 3 cycles. The cytokines expression level was lower in both in vitro cell stimulation and in vivo animal experiments than in untreated group (P Prevotella intermedia.

  11. Evaluation of fixative solutions for ultrastructural analysis of brown spider Loxosceles intermedia (araneae: sicariidae) tissues

    OpenAIRE

    Costa-Ayub,C. L. S.; Faraco,C. D.; Freire,C. A.

    2006-01-01

    In view of the widely varying compositions of fixative solutions used for studying spiders, five different fixative formulas were tested for fixing male brown-spider (Loxosceles intermedia) gonad tissues. The brown spider represents a public health problem in Curitiba (Paraná State, Brazil). Morphological study of its gonads may aid in understanding the reproductive strategies of this species, and possibly in developing a reproduction control program. The fixatives tested contained glutaralde...

  12. Gibberella intermedia the pathogen of St. John's Wort, coneflower and marshmallow in Serbia

    Directory of Open Access Journals (Sweden)

    Pavlović Snežana Đ.

    2009-01-01

    Full Text Available Gibberella intermedia (Kuhlmann Samuels et al. (anamorf: Fusarium proliferatum /Matsushima/ Nirenberg was isolated from seeds of St. John's wort, marshmallow, and coneflower, as well as from roots and stalks of marshmallow and roots of coneflower. These plants had symptoms of leaf chlorosis, malformation, withering and plant dwarfing and were collected from several localities in Serbia during five-year investigations of mycopopulations of the mentioned plants. The morphological characteristics of the pathogen were described.

  13. Gibberella intermedia the pathogen of St. John's Wort, coneflower and marshmallow in Serbia

    OpenAIRE

    Pavlović Snežana Đ.; Stojšin Vera B.; Stojanović Saša D.; Starović Mira S.; Bagi Ferenc F.; Budakov Dragana B.

    2009-01-01

    Gibberella intermedia (Kuhlmann) Samuels et al. (anamorf: Fusarium proliferatum /Matsushima/ Nirenberg) was isolated from seeds of St. John's wort, marshmallow, and coneflower, as well as from roots and stalks of marshmallow and roots of coneflower. These plants had symptoms of leaf chlorosis, malformation, withering and plant dwarfing and were collected from several localities in Serbia during five-year investigations of mycopopulations of the mentioned plants. The morphological characterist...

  14. DHA suppresses Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages.

    Science.gov (United States)

    Choi, Eun-Young; Jin, Ji-Young; Choi, Jeom-Il; Choi, In Soon; Kim, Sung-Jo

    2014-04-14

    Several reports have indicated that dietary intake of DHA is associated with lower prevalence of periodontitis. In the present study, we investigated the effect of DHA on the production of proinflammatory mediators in murine macrophage-like RAW264.7 cells stimulated with lipopolysaccharide (LPS) isolated from Prevotella intermedia, a pathogen implicated in inflammatory periodontal disease, and its mechanisms of action. LPS was isolated from lyophilised P. intermedia ATCC 25,611 cells using the standard hot-phenol-water protocol. Culture supernatants were collected and assayed for NO, IL-1β and IL-6. Real-time PCR analysis was carried out to detect the expression of inducible NO synthase (iNOS), IL-1β, IL-6 and haeme oxygenase-1 (HO-1) mRNA. Immunoblot analysis was carried out to quantify the expression of iNOS and HO-1 protein and concentrations of signalling proteins. DNA-binding activities of NF-κB subunits were determined using an ELISA-based assay kit. DHA significantly attenuated the production of NO, IL-1β and IL-6 at both gene transcription and translation levels in P. intermedia LPS-activated RAW264.7 cells. DHA induced the expression of HO-1 in cells treated with P. intermedia LPS. Selective inhibition of HO-1 activity by tin protoporphyrin IX significantly mitigated the inhibitory effects of DHA on LPS-induced NO production. DHA significantly attenuated the phosphorylation of c-Jun N-terminal kinase induced by LPS. In addition, DHA suppressed the transcriptional activity of NF-κB by regulating the nuclear translocation and DNA-binding activity of NF-κB p50 subunit and inhibited the phosphorylation of signal transducer and activator of transcription 1. Further in vivo studies are needed to better evaluate the potential of DHA in humans as a therapeutic agent to treat periodontal disease.

  15. Cloning and Biochemical Characterization of a Class A β-Lactamase from Prevotella intermedia

    Science.gov (United States)

    Madinier, I.; Fosse, T.; Giudicelli, J.; Labia, R.

    2001-01-01

    The gene encoding a β-lactamase of Prevotella intermedia was cloned and sequenced. This gene, called cfxA2, shared 98% identity with cfxA, the structural gene of a β-lactamase previously described in Bacteroides vulgatus. The deduced protein sequence had a K272E substitution. CfxA2 had the characteristics of class A, group 2e β-lactamases. PMID:11451705

  16. Anti-inflammatory and antinociceptive effects of the stem bark of Byrsonima intermedia A. Juss.

    Science.gov (United States)

    Orlandi, Lidiane; Vilela, Fabiana C; Santa-Cecília, Flávia V; Dias, Danielle F; Alves-da-Silva, Geraldo; Giusti-Paiva, Alexandre

    2011-10-11

    Byrsonima intermedia A. Juss. is popularly known as "murici pequeno" and is native to the Brazilian Cerrado. This species has been used as an antimicrobial, anti-hemorrhagic, anti-diarrheal and anti-inflammatory. Nevertheless, scientific information regarding Byrsonima intermedia is limited; there are no reports related to its possible anti inflammatory and antinociceptive effects. This study employed in vivo inflammatory and nociceptive models to evaluate the scientific basis for the traditional use of Byrsonima intermedia. Carrageenan-induced paw edema, peritonitis and fibrovascular tissue growth induced by s.c. cotton pellet implantation tests were used to investigate the anti-inflammatory activity of Byrsonima intermedia aqueous extract (BiAE) in rats. Mechanical nociceptive paw, formalin and hot plate tests were used to evaluate the antinociceptive activity in mice. High-performance liquid chromatography (HPLC), phytochemistry screening and determination of total phenolics and flavonoids were used to determine the chemical profile of the BiAE. BiAE at test doses of 30-300 mg/kg p.o. clearly demonstrated anti-inflammatory effects by reduced carrageenan-induced paw edema, by inhibited leukocyte recruitment into the peritoneal cavity and, in the model of chronic inflammation, by using the cotton pellet-induced fibrovascular tissue growth in rats. The extracts at test doses of 30-300 mg/kg p.o. clearly demonstrated antinociceptive activity in all tests. Administration of the opioid receptor antagonist naloxone completely inhibited the antinociceptive effect induced by BiAE (100 mg/kg). BiAE markedly exhibits anti-inflammatory action in rats and antinociceptive activity in mice. Thus, it may be useful in the treatment of inflammatory hyperalgesic disorders, which supports previous claims of its traditional use. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  17. Terapia de la risa en un grupo de mujeres adultas/ Laughter therapy in a group of elderly women

    Directory of Open Access Journals (Sweden)

    María M. Villam

    2013-05-01

    Full Text Available Objetivo: explorar los beneficios de la terapia de la risa en un grupo de mujeres adultas pertenecientes a un centro gerontológico del Municipio de Envigado (Colombia. Metodología: estudio con enfoque cualitativo mediante entrevista semiestructurada a 10 adultas entre 59 y 97 años de edad, antes y después de la aplicación de 5 sesiones, una semanal, de terapia de la risa por parte del grupo Mediclaun “payasos hospitalarios”. Resultados: muchos de los cambios percibidos en las adultas coinciden con la literatura en lo que respecta a una actitud más positiva, incremento de la confianza en el otro y la aceptación; expresan el pasado con menos dolor y un mayor agrado frente al acompañamiento familiar. Conclusiones: La terapia de la risa permite al adulto mayor encontrar fortalezas que puedan ayudarle a mejorar su presente, a su vez, lograr cambios en el individuo que se le revierten positivamente y a su relación con el entorno Objective: to explore the benefits of laughter therapy in a group of elderly women belonging to a gerontology center (Envigado, Colombia. Methodology: a qualitative study using a semi-structured interview with 10 adult women aged 59 to 97. The participants were interviewed before and after applying 5 weekly sessions of laughter therapy. Said therapy was administered by the Mediclaun "hospital clowns" group. Results: many of the changes observed in adult women are consistent with the results described in the literature in relation to a more positive attitude, increased confidence in people and acceptance; the participants expressed the past with less pain and had a greater liking towards family support. Conclusions: laughter therapy allows seniors to find the strength to improve their present situation, thus causing positive changes in their own self and in their relationship with their surroundings

  18. Experiencias de profesionales de enfermería en terapias alternativas y complementarias aplicadas a personas en situaciones de dolor

    Directory of Open Access Journals (Sweden)

    BLANCA CECILIA VANEGAS DE AHOGADO

    2008-07-01

    Full Text Available Para conocer las experiencias de profesionales de enfermería en el uso de terapias alternativas y complementarias aplicadas durante el cuidado de la salud a personas en situación de dolor, se realizó un estudio cualitativo, como trabajo de grado de un grupo de estudiantes -de pregrado- de Enfermería de la Uni-versidad El Bosque, entre los años 2005 y 2007, en el que participaron cuatro profesionales de enfermería, con varios años de experiencia en la utilización de Terapia Floral, Acupuntura, Homeopatía, Quiromasaje y Naturopatía; es decir, aquellas terapias que, además permiten una acción sinérgica al combinarse con los procedimientos terapéuticos médico-farmacológicos-quirúrgicos convencionales, a los cuales complementan; la selección de participantes se hizo mediante la técnica de bola de nieve. La información se recogió a través de entrevistas profundas semiestructuradas. El análisis de los resultados, permitió conocer que dichas experiencias no tienen como único propósito aliviar determinado tipo de dolor, sino, por lo general, valorar el estado de salud y hacer las intervenciones con enfoque integral, considerando a la persona como un ser holístico; por otra parte, este trabajo les ha proporcionado a las participantes gran satisfacción y posibilidades de desarrollo personal.

  19. Efectividad de la terapia cognitivo-conductual en la pérdida de peso tras dos años de cirugía bariátrica en pacientes con obesidad mórbida

    OpenAIRE

    V Abilés; J Abilés; S Rodríguez-Ruiz; V Luna; F Martín; N Gándara; MC Fernández-Santaella

    2013-01-01

    Objetivo: Evaluar la efectividad de la Terapia Cognitivo Conductual (TCC) en el éxito de la pérdida de peso postoperatoria tras 2 años de CB. Metodología: Estudio observacional prospectivo en el que se incluyeron pacientes entre 18 y 59 años candidatos a CB, entre enero de 2007 y junio de 2010, realizando seguimiento postoperatorio hasta junio de 2012. Se compararon dos grupos de estudio de acuerdo a su participación en TCC o no. Se tomaron datos de peso corporal y estatura, a partir de las c...

  20. Equine pituitary pars intermedia dysfunction: current perspectives on diagnosis and management

    Directory of Open Access Journals (Sweden)

    Spelta CW

    2015-08-01

    Full Text Available Caroline W Spelta Townsville Vet Clinic, Townsville, QLD, Australia Abstract: Equine pituitary pars intermedia dysfunction (PPID is a neurodegenerative disease of the hypothalamus, resulting in the loss of dopaminergic inhibition of pars intermedia. An oxidative stress injury of unknown etiology has been suggested to initiate the neurodegeneration. While hypertrichosis (formerly known as hirsutism is considered pathognomic for advanced disease, the antemortem diagnosis of subclinical and early disease has continued to prove difficult. Numerous tests have been used with varying sensitivities and specificities. The overnight dexamethasone suppression test, originally documented to have 100% sensitivity and specificity in horses with advanced disease, has proven to be less valuable in identifying early disease. Basal plasma adrenocorticotropin concentrations have improved sensitivity and specificity when sampled during the autumn months, and α-melanocyte-stimulating hormone, while not yet commercially available, shows promise as a sensitive and specific single sample test. Recent advances in our knowledge include the strong association between laminitis and hyperinsulinemia, both common clinical signs associated with PPID. The pathogenesis of hyperinsulinemia, laminitis, and their association with this disease is a focus of current research. The dopamine agonist pergolide mesylate is still the mainstay of medical management, with studies on oral bioavailability, pharmacokinetics, and long-term survival rates now published. Keywords: PPID, ACTH, α-MSH, laminitis, pergolide, hypertrichosis, pars pituitary intermedia dysfunction

  1. Effect of pollen irradiation on hybridization of wheat and Eltrigia intermedia and their embryo development

    International Nuclear Information System (INIS)

    Li Guiying; Wang Linqing; Shi Jinguo

    2005-01-01

    Spikes of Eltrigia intermedia were radiated with 5-100 Gy γ-ray during anthesis, and then their pollens were collected to pollinate to the common wheat 'J-11' and 'Chinese Spring'. The effects of pollen irradiation on the seed setting, embryo development, embryo culture and plantlet rate were studied. The results showed that low dose (5-9 Gy) of irradiation enhanced the seed setting for Chinese spring x E. intermedia, but no such effect for J-11xE. intermedia. Irradiation with all doses damaged embryo development, percentage of seeds with embryos; rate of immature hybrid embryos developing into plantlets decreases with the increased doses. Percentage of seeds with abnormal embryos increased significantly with the doses. 12.9%-14.5% of embryos could develop into plants in 30 Gy treatment, which seldom occur to wheat. Embryos in 50 Gy-100 Gy treatment were affected so serious that even none of them could develop into plants in vitro culture. It may be an effective approach to obtaining more mutants and enhancing induced mutation breeding by using combination of pollen irradiation, immature embryo rescue and anther culture of the resultant progenies. (authors)

  2. AdpC is a Prevotella intermedia 17 leucine-rich repeat internalin-like protein.

    Science.gov (United States)

    Iyer, Divya; Anaya-Bergman, Cecilia; Jones, Kevin; Yanamandra, Sai; Sengupta, Dipanwita; Miyazaki, Hiroshi; Lewis, Janina P

    2010-06-01

    The oral bacterium Prevotella intermedia attaches to and invades gingival epithelial cells, fibroblasts, and endothelial cells. Several genes encoding proteins that mediate both the adhesion and invasion processes are carried on the genome of this bacterium. Here, we characterized one such protein, AdpC, belonging to the leucine-rich repeat (LRR) protein family. Bioinformatics analysis revealed that this protein shares similarity with the Treponema pallidum LRR (LRR(TP)) family of proteins and contains six LRRs. Despite the absence of a signal peptide, this protein is localized on the bacterial outer membrane, indicating that it is transported through an atypical secretion mechanism. The recombinant form of this protein (rAdpC) was shown to bind fibrinogen. In addition, the heterologous host strain Escherichia coli BL21 expressing rAdpC (V2846) invaded fibroblast NIH 3T3 cells at a 40-fold-higher frequency than control E. coli BL21 cells expressing a sham P. intermedia 17 protein. Although similar results were obtained by using human umbilical vein endothelial cells (HUVECs), only a 3-fold-increased invasion of V2846 into oral epithelial HN4 cells was observed. Thus, AdpC-mediated invasion is cell specific. This work demonstrated that AdpC is an important invasin protein of P. intermedia 17.

  3. AdpC Is a Prevotella intermedia 17 Leucine-Rich Repeat Internalin-Like Protein▿

    Science.gov (United States)

    Iyer, Divya; Anaya-Bergman, Cecilia; Jones, Kevin; Yanamandra, Sai; Sengupta, Dipanwita; Miyazaki, Hiroshi; Lewis, Janina P.

    2010-01-01

    The oral bacterium Prevotella intermedia attaches to and invades gingival epithelial cells, fibroblasts, and endothelial cells. Several genes encoding proteins that mediate both the adhesion and invasion processes are carried on the genome of this bacterium. Here, we characterized one such protein, AdpC, belonging to the leucine-rich repeat (LRR) protein family. Bioinformatics analysis revealed that this protein shares similarity with the Treponema pallidum LRR (LRRTP) family of proteins and contains six LRRs. Despite the absence of a signal peptide, this protein is localized on the bacterial outer membrane, indicating that it is transported through an atypical secretion mechanism. The recombinant form of this protein (rAdpC) was shown to bind fibrinogen. In addition, the heterologous host strain Escherichia coli BL21 expressing rAdpC (V2846) invaded fibroblast NIH 3T3 cells at a 40-fold-higher frequency than control E. coli BL21 cells expressing a sham P. intermedia 17 protein. Although similar results were obtained by using human umbilical vein endothelial cells (HUVECs), only a 3-fold-increased invasion of V2846 into oral epithelial HN4 cells was observed. Thus, AdpC-mediated invasion is cell specific. This work demonstrated that AdpC is an important invasin protein of P. intermedia 17. PMID:20308299

  4. Cleavage of CD14 and LBP by a protease from Prevotella intermedia

    Science.gov (United States)

    Deschner, James; Singhal, Anuradha; Long, Ping; Liu, Chau-Ching; Piesco, Nicholas

    2016-01-01

    Periodontitis is an inflammatory disease caused by subgingival microorganisms and their components, such as lipopolysaccharide (LPS). Responses of the host to LPS are mediated by CD14 and LPS-binding protein (LBP). In this study, it was determined that proteases from a periodontal pathogen, Prevotella intermedia, cleave CD14 and LBP, and thereby modulate the virulence of LPS. Culture supernatants from two strains of P. intermedia (ATCC 25611 and 25261) cleaved CD14 and LBP in a concentration-dependent manner. Zymographic and molecular mass analysis revealed the presence of a membrane-associated, 170-kDa, monomeric protease. Class-specific inhibitors and stimulators demonstrated that this enzyme is a metal-requiring, thiol-activated, cysteine protease. The protease was stable over a wide range of temperatures (4–56 °C) and pH values (4.5–8.5). This enzyme also decreased the expression of interleukin-1β (IL-1β)-specific mRNA in the LPS-activated macrophage-like cell lines U937 and THP-1 in a concentration-dependent manner, indicating that it also cleaves membrane-associated CD14. Furthermore, addition of soluble CD14 abrogated protease-mediated inhibition of IL-1 mRNA expression induced by LPS. The observations suggest that proteolysis of CD14 and LBP by P. intermedia protease might modulate the virulence of LPS at sites of periodontal infections. PMID:12728301

  5. Del Distrito Industrial al Desarrollo Territorial: estrategias de innovación en ciudades intermedias

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    Ricardo Méndez

    2006-01-01

    Full Text Available Los contrastes territoriales se ha incrementado desde el inicio de los procesos de globalización e integración, pero, al mismo tiempo, las ciudades están mostrando diferente capacidad de respuesta ante esas transformaciones. Dentro de la Unión Europea, el policentrismo y el papel de las ciudades intermedias han cobrado creciente importancia en la actual agenda política, especialmente en el caso de las regiones periféricas con problemas estructurales que dificultan su desarrollo. El artículo hace una revisión crítica del paradigma de los distritos industriales y los ambientes innovadores para centrarse en su posible utilidad en la búsqueda de las claves para la construcción de ciudades intermedias innovadoras. Se concede especial importancia a los actores locales, las redes institucionales, la organización de sistemas productivos locales y la gobernabilidad territorial. Por último, se plantean algunas propuestas para apoyar la consolidación de procesos innovativos en ciudades intermedias.

  6. Terapia antiviral para VIH-SIDA

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    Alicia Tarinas Reyes

    2000-12-01

    Full Text Available En los últimos años, muchos agentes antivirales nuevos han sido incorporados a la quimioterapéutica. En esta revisión se resumen tanto los fármacos establecidos de años atrás como los nuevos medicamentos desarrollados para el tratamiento de individuos infectados por VIH. El AZT fue el primero aprobado en marzo de 1987, le siguió el ddl (1991, ddC (1992, d4T (1994, 3TC (1995. Luego fue aprobado el primer inhibidor de proteasa, saquinavir en diciembre de 1995, seguido de ritonavir (1996, indinavir (1996, nelfinavir (1997; además de otros inhibidores de la reverso transcriptasa como nevirapine (1996, delavirdine (1997, efavirenz (1998, entre otros. En estos momentos se siguen buscando y desarrollando nuevas terapias alternativas para esta afección. En este trabajo se exponen algunas de las características de dichos medicamentos, como son: mecanismos de acción (sobre qué enzima actúa cada uno y cómo lo hacen, el ciclo viral, dosificación, incompatibilidades y reacciones adversas.During the last years many new antiviral agents have been incorporated to the chemotherapeutics. The pharmaceuticals established years ago as well as the new ones developed to treat HIV infected individuals are included in this review. The AZT was the first approved in March, 1987, followed by ddl (1991, ddc (1992, d4t (1994, and 3TC (1995. Later, the first protease inhibitor, saquinovir, was approved in December, 1995, followed by ritonavir (1996, indinavir (1996, and nelfinavir (1997; in addition to other inhibitors of the reverse transcriptase as neviparine (1996, delavirdine (1997, and efavirenz (1998, among others. At present new alternative therapies for this affection are being searched and developed. Some of the characteristics of these dugs, such as: action mechanisms (on which enzime each of them act and how they do it, viral cycle, dosage, incompatibilites and adverse reactions are dealt with in this paper.

  7. Vocabulario inglés-español de productos de apoyo utilizados en terapia ocupacional para personas con enfermedades neuromusculares

    OpenAIRE

    Tamara Varela Vila; Elena Sánchez Trigo; Valentina Marta Rodríguez

    2012-01-01

    En este trabajo presentamos el vocabulario bilingüe (inglés-español) de productos de apoyo utilizados en terapia ocupacional para personas con enfermedades neuromusculares que hemos elaborado, así como los criterios y la metodología seguidos. Los productos de apoyo son aquellos elementos y tecnologías que permiten aumentar las capacidades funcionales de los afectados. El objetivo de este estudio es doble. Por un lado, contribuir a la difusión de la terminología propia de un ámbito muy novedos...

  8. Lumbalgia mecánica crónica y terapia manual ortopédica. A propósito de un caso

    OpenAIRE

    Martínez Varona, Silvia

    2013-01-01

    Se trata de un estudio sobre lumbalgia mecánica crónica y su abordaje de tratamiento respecto a la terapia manual ortopédica. Para ello, se analiza un caso clínico en el que se estudian los resultados e un tratamiento basado en técnicas articulares y de estabilización muscular. Para finalizar se hace un repaso actualizado en cuanto a la bibliografía encontrada sobre el tema y se llegan a unas conclusiones determinadas. Grado en Fisioterapia

  9. Manejo de la sialorrea en personas con enfermedad de Parkinson mediante terapia conductivo conductual y estimulación termo-táctil

    OpenAIRE

    Tapia, S.

    2014-01-01

    Este estudio propone un tratamiento fonoaudiológico para trabajar con personas que presentan sialorrea (producción de saliva que el paciente percibe como excesiva) producto de la Enfermedad de Parkinson (EP). Dieciocho personas con diagnóstico de EP y sialorrea, las cuales se dividieron en dos grupos; A: recibió sólo Terapia Cognitivo Conductual (TCC) (concientización del proceso deglutorio), mientras el B: recibió TCC más Estimulación Termo Táctil (ETT) (se realiza estimulación sensorial con...

  10. Efectividad de la terapia de liberación miofascial en el tratamiento de la cervicalgia mecánica en el ámbito laboral

    OpenAIRE

    Rodríguez Fuentes, Iván

    2013-01-01

    [Resumen] El dolor cervical presenta una elevada prevalencia y repercute significativamente en el ámbito laboral y social de los individuos que lo padecen. Considerando el interés surgido por el sistema fascial como elemento vertebrador corporal y dada la escasez de estudios que muestren la efectividad de su tratamiento, el objetivo de esta investigación fue evaluar la efectividad y el coste-efectividad de la terapia de liberación miofascial en pacientes con cervicalgia mecánica laboral re...

  11. Enfermeiros especialistas em Terapia Nutricional no Brasil: onde e como atuam Enfermeros especalistas en Terapia Nutricional en Brasil: donde y como estan actuando Nurse specialists in Nutritional Therapy in Brasil: where and how they are acting

    Directory of Open Access Journals (Sweden)

    Deolinda Marçal Vieira dos Santos

    2006-12-01

    Full Text Available A desnutrição hospitalar prevalece. Dentre 105.841 enfermeiros registrados no COFEN, 47 são especialistas em Terapia Nutricional (0,04%. Objetivos: caracterizar a população e saber como e onde atuam, se há aplicabilidade da legislação na prática. Sujeitos: enfermeiros especialistas pela Sociedade Brasileira de Nutrição Parenteral e Enteral. Estudo descritivo cujo instrumento foi um questionário aplicado. Resultados: Participação de 57,4% da população, 85,2% graduaram-se em escolas públicas; na década de 80 59,3% realizaram habilitação, sendo 37,4% em Terapia Nutricional 3,7%; 63% realizaram capacitação lato sensu 63% em áreas diferentes da Terapia Nutricional, 11,1% realizaram capacitação strictu sensu em Terapia Nutricional. 66,7% atuam em hospitais, sendo que 59,2% atuam direta ou indiretamente com Terapia Nutricional, enfermeiros assistenciais 44,0%. 85,2% aplicam a legislação na prática. Conclusão: a população é representativa, porém insuficiente para a demanda no país. Faz-se necessário investigar quem está coordenando e qual equipe administra a Terapia Nutricional.Desnutrição hospitalar domina. Hay no Brasil, 47 expertos en Terapia Nutricional entre 105.841 enfermeros registrados en el COFEN (0.04%. Objetivos: caracterizar la población, saber como y donde actúan, si hay aplicabilidad de la legislación práctica. Ciudadanos: enfermeros expertos en Terapia Nutricional por la Sociedad Brasilera de Nutrición Parenteral e Enteral. Estudio descriptivo con aplicación de cuestionario. Resultados: Participación de 57.4% de la población, 85.2% se graduaron en escuelas públicas, 59.3% en la década de 80. Se calificaron 37.4%. El 3.7% de la muestra en Terapia Nutricional. 63% obtuve capacitación lato sensu en áreas distintas de la Terapia Nutricional. El 11.1% obtuvo capacitación strictu sensu en Terapéutica Nutricional. Actuan en el hospital 66,7%, de manera directa o indirectamente con la Terapia

  12. ALCOOLISMO NO CONTEXTO FAMILIAR: ESTRATÉGIAS DE ENFRENTAMENTO DAS IDOSAS USUÁRIAS DA TERAPIA COMUNITÁRIA

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    Maria de Oliveira Ferreira Filha

    2012-01-01

    Full Text Available El alcoholismo en el contexto familiar genera daños significativos a la mujer anciana que se torna la víctima más evidente de esta problemática. Estudio documental, realizado en la Secretaria de Salud de João Pessoa, Paraíba, en julio/2008, con objetivo de identificar estrategias utilizadas por las ancianas para enfrentarse al alcoholismo en la familia y las hablas espontáneas expresadas en el último momento las ruedas de la terapia. Fueron consultadas 776 fichas de informaciones de la terapia comunitaria integradora. Los hallazgos fueron organizados en narraciones y divididos en categorías de análisis. Las estrategias más utilizadas fueron: espiritualidad (33,3%, grupos de autoayuda (26,6% y fortalecimiento de las familias (13,3%; y las hablas más frecuentes: coraje (15,5%, esperanza (13,3%, fe (11,1%, confianza y unión (6,6%. La relación entre los resultados apunta como efecto positivo el empoderamiento, fortalecimiento de lazos y la resiliencia, permitiendo a la familia la superación de los dilemas cotidianos delante del alcoholismo.

  13. Terapia cognitiva-conductual (TCC Como tratamiento para la depresion: Una revisión del estado del arte

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    Juliana Vanessa Puerta Polo

    2013-10-01

    Full Text Available ResumenLa terapia cognitivo - conductual (TCC se ha transformado en una de las estrategias por excelencia de tipo no farmacológico para el tratamiento de la depresión. A través del presente estudio se brinda una visión general de las técnicas centrales que los teóricos de la TCC expusieron a través de los últimos 50 años, y que se han estado implementado para el abordaje de dicho trastorno mental. De igual manera, se pretendió informar al lector acerca de las innovaciones y adaptaciones más recientes de este modelo de psicoterapia. Se concluye que la TCC ha demostrado su efectividad en el tratamiento de la depresión, por lo cual debe promoverse este tipo de terapia. (DUAZARY 2011 No. 2, 251 - 257AbstractThe cognitive - behavioral therapy (CBT has become one of the strategies for excellence in non-pharmacological treatment of depression. Through this study provided an overview of the theorists core techniques of CBT exposed through last 50 years and have been implemented for addressing the mental disorder. Similarly, it is intended to inform the reader about the latest innovations and adaptations of this model of psychotherapy. We conclude that CBT has been proven effective in the treatment of depression, which should promote this type of therapy.Keywords: cognitive-behavioral therapy; depression; review.

  14. Genome sequence of Prevotella intermedia SUNY aB G8-9K-3, a biofilm forming strain with drug-resistance.

    Science.gov (United States)

    Moon, Ji-Hoi; Kim, Minjung; Lee, Jae-Hyung

    Prevotella intermedia has long been known to be as the principal etiologic agent of periodontal diseases and associated with various systemic diseases. Previous studies showed that the intra-species difference exists in capacity of biofilm formation, antibiotic resistance, and serological reaction among P. intermedia strains. Here we report the genome sequence of P. intermedia SUNY aB G8-9K-3 (designated ATCC49046) that displays a relatively high antimicrobial resistant and biofilm-forming capacity. Genome sequencing information provides important clues in understanding the genetic bases of phenotypic differences among P. intermedia strains. Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

  15. Genome sequence of Prevotella intermedia SUNY aB G8-9K-3, a biofilm forming strain with drug-resistance

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    Ji-Hoi Moon

    Full Text Available Abstract Prevotella intermedia has long been known to be as the principal etiologic agent of periodontal diseases and associated with various systemic diseases. Previous studies showed that the intra-species difference exists in capacity of biofilm formation, antibiotic resistance, and serological reaction among P. intermedia strains. Here we report the genome sequence of P. intermedia SUNY aB G8-9K-3 (designated ATCC49046 that displays a relatively high antimicrobial resistant and biofilm-forming capacity. Genome sequencing information provides important clues in understanding the genetic bases of phenotypic differences among P. intermedia strains.

  16. Effect of quercetin on the production of nitric oxide in murine macrophages stimulated with lipopolysaccharide from Prevotella intermedia.

    Science.gov (United States)

    Cho, Yun-Jung; Kim, Sung-Jo

    2013-08-01

    Nitric oxide (NO) is a short-lived bioactive molecule that is known to play an important role in the pathogenesis of periodontal disease. In the current study, we investigated the effect of the flavonoid quercetin on the production of NO in murine macrophages activated with lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen related to inflammatory periodontal disease, and tried to elucidate the underlying mechanisms of action. LPS was isolated from P. intermedia ATCC 25611 cells by the standard hot phenol-water method. The concentration of NO in cell culture supernatants was determined by measuring the accumulation of nitrite. Inducible NO synthase (iNOS) and heme oxygenase-1 (HO-1) protein expression, phosphorylation of c-Jun N-terminal kinase (JNK) and p38, inhibitory κB (IκB)-α degradation, and signal transducer and activator of transcription 1 (STAT1) phosphorylation were analyzed via immunoblotting. Quercetin significantly attenuated iNOS-derived NO production in RAW246.7 cells activated by P. intermedia LPS. In addition, quercetin induced HO-1 protein expression in cells activated with P. intermedia LPS. Tin protoporphyrin IX (SnPP), a competitive inhibitor of HO-1, abolished the inhibitory effect of quercetin on LPS-induced NO production. Quercetin did not affect the phosphorylation of JNK and p38 induced by P. intermedia LPS. The degradation of IκB-α induced by P. intermedia LPS was inhibited when the cells were treated with quercetin. Quercetin also inhibited LPS-induced STAT1 signaling. Quercetin significantly inhibits iNOS-derived NO production in murine macrophages activated by P. intermedia LPS via anti-inflammatory HO-1 induction and inhibition of the nuclear factor-κB and STAT1 signaling pathways. Our study suggests that quercetin may contribute to the modulation of host-destructive responses mediated by NO and appears to have potential as a novel therapeutic agent for treating inflammatory periodontal disease.

  17. Characteristics of Prevotella intermedia-specific CD4+ T cell clones from peripheral blood of a chronic adult periodontitis patient

    Science.gov (United States)

    Wassenaar, A; Reinhardus, C; Abraham-Inpijn, L; Snijders, A; Kievits, F

    1998-01-01

    Periodontitis is a chronic destructive inflammatory disease associated with periodontopathic bacteria. In addition, autoantigens such as collagen and heat shock proteins (hsp) have been suggested to play a role. Established periodontal lesions are characterized by dense infiltrations of immune cells such as cytokine-producing CD4+ and CD8+ T cells. CD4+ T cells specific for Prevotella intermedia can be isolated from lesional gingiva, suggesting an active role for CD4+ T cells in the response to this bacterium. We therefore investigated the characteristics of a panel of 13 P. intermedia-specific CD4+ T cells generated from the peripheral blood of a patient with chronic adult periodontitis. All 13 P. intermedia-specific CD4+ T cells recognized the antigens in the context of HLA-DR. The T cell clones were mainly classified as Th0, producing comparable amounts of interferon-gamma (IFN-γ) and IL-4, and Th2, producing high amounts of IL-4 and almost no IFN-γ. None of the P. intermedia-specific T cell clones recognized antigens of the periodontopathic bacteria Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis and of the autoantigens collagen and hsp. The reactivity profile of the T cell clones to size-fractionated cell envelope antigens of P. intermedia indicated that P. intermedia-specific CD4+ T cell clones recognize probably five different antigen specificities in the context of the MHC class II molecules, DR7 or DR15. These results suggest that a broad panel of cell-associated protein antigens play a role in the induction of P. intermedia-specific CD4+ T cell response. PMID:9697992

  18. Anti-inflammatory effect of extract and fractions from the leaves of Byrsonima intermedia A. Juss. in rats.

    Science.gov (United States)

    Moreira, Lucimara Q; Vilela, Fabiana C; Orlandi, Lidiane; Dias, Danielle F; Santos, Ana Laura A; da Silva, Marcelo A; Paiva, Renato; Alves-da-Silva, Geraldo; Giusti-Paiva, Alexandre

    2011-11-18

    Byrsonima intermedia is commonly used for its antiseptic, antimicrobial, and anti-inflammatory properties in the treatment of diarrhea and dysentery in Brazilian folk medicine. The purpose of this study was to examine the anti-inflammatory activity of the aqueous extract and fractions of Byrsonima intermedia leaves. Rats with carrageenan-induced paw edema and fibrovascular tissue growth, which was induced by subcutaneous implantation of a cotton pellet, were used as acute and chronic animal models of inflammation to investigate the anti-inflammatory effects of the aqueous extract and the individual ethyl acetate (EtOAc) and aqueous fractions of Byrsonima intermedia and catechin. High-performance liquid chromatography (HPLC) was used to determine the fingerprint chromatogram of the aqueous extract and fractions of Byrsonima intermedia. The crude aqueous extract at test doses of 30-300 mg/kg p.o. clearly demonstrated anti-inflammatory effects by reducing carrageenan-induced paw edema, as did the ethyl acetate (100mg/kg) and aqueous fractions (30-100mg/kg). In the chronic inflammation rat animal model with fibrovascular tissue growth, the aqueous extract of Byrsonima intermedia (BiAE) at doses of 30-300 mg/kg and the individual EtOAc and aqueous fractions at doses of 30-100mg/kg and catechin significantly reduced the formation of granulomatous tissue. The presence of catechin and phenolic compounds in the extract and fractions of Byrsonima intermedia was confirmed using HPLC. BiAE and the individual EtOAc and aqueous fractions of Byrsonima intermedia exhibited chronic and acute anti-inflammatory efficacy in rats, which supports previous claims of its use in traditional medicine. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  19. Complicações decorrentes da terapia intravenosa em pacientes cirúrgicos Complicaciones causadas por la terapia intravenosa en pacientes quirúrgicos Complications due to intravenous therapy in surgical patients

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    Renata Cristina de Campos Pereira

    2000-10-01

    Full Text Available A enfermagem desempenha papel primordial na prevenção e redução das complicações relacionadas ao acesso venoso. O estudo teve como objetivo o levantamento das complicações decorrentes da terapia intravenosa. Os dados de maior expressividade obtidos foram: 77,3% das punções foram realizadas pelo auxiliar de enfermagem; 68% não usaram luvas durante o procedimento; 60% das punções tiveram cuidado de enfermagem insatisfatório; 47% dos dispositivos permaneceram "in situ" de 24 a 72 horas; dentre as complicações, 20% relacionaram-se com infiltração, 5,4% infiltração e hematoma e 5,3% obstrução. Os dados sugerem a necessidade de um aprimoramento da equipe de enfermagem relacionado a terapia intravenosa.Enfermería desempeña un papel primordial en la prevención y reducción de complicaciones relacionadas con el acceso venoso. El estudio tiene como objetivo la identificación de complicaciones ocurridas en la terapia intravenosa. Los datos más relevantes que fueron obtenidos son: 77,3% de las punciones fueron realizadas por auxiliares de enfermería; 68% no usaron guantes durante el procedimiento; 60% de las punciones tuvieron cuidado de enfermería insatisfactorio; 20% relacionados con infiltraciones; 5,4% con infiltraciones y hematomas y, 5,3% con obstrucciones. Los datos sugieren la necesidad de capacitar al equipo de enfermería con respecto a la terapia intravenosa.Nursing plays an important role in prevention and reduction of complications related to venous access. This study had the purpose to find complications derived from intravenous therapy. Major data were: 77.3% of the venous accesses were performed by auxiliary nursing; 68% of the procedures were performed without gloves; 60% of the accesses were not satisfactorily performed by nurses; 47% of the cannulae were "in situ" between 24 and 72 hours; among the complications, 20% were related to infiltration, 5.4% to infiltration and hematoma and 5.3% to obstruction. Data

  20. Ressincronização cardíaca e terapia celular: existe terapia associativa?

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    Oswaldo T. Greco

    Full Text Available Nos últimos anos, a terapia de ressincronização cardíaca tem sido uma conduta bem definida para pacientes com insuficiência cardíaca (IC, classe funcional III ou IV, que não tiveram resposta à otimização terapêutica. Estudos já mostraram esta eficácia, mas um grupo bem definido de miocardiopatias dilatadas com áreas de fibrose não tem tido o mesmo sucesso (30%-40%. Por isto decidimos associar a estes pacientes o implante de células-tronco. A partir de 04/2005 iniciamos estes implantes em pacientes (pcs. com IC, classe funcional III/IV, otmizados e fração de ejeção <35% com dissincronia ventricular. Total de 25 pacientes (16 homens, idade entre 30-80anos (média de 58 e 44% de etiologia chagásica (11 pcs. Ressincronização e terapia celular foram por via epicárdica (12 pcs e endocárdica (13 pcs e a seleção celular pela filtração por Ficoll e 3.0x10(8 de células infundidas CD34 + (5,0 x 10(6 e CD 133 + (2,5 x 10(6. Após 24 meses de evolução observamos que 84,8% destes pcs permaneceram vivos e a mortalidade (15,2% não estava relacionada ao procedimento. A sobrevida dos portadores de marcapasso foi maior (52,4% e os chagásicos tiveram uma pior sobrevida, mas com teste de long-rank de 0,218 e 0,626, respectivamente. Nos casos de pcs com ressincronizador isoladamente, os resultados não parecem ser tão bons,e nós optamos pela associação de células-tronco como uma alternativa segura e adequada para estes graves pcs com insuficiência cardíaca e miocardiopatia dilatada.

  1. ENFOQUE TEÓRICO Y CONCEPTUAL PARA EL ESTUDIO DE LAS URBANIZACIONES CERRADAS

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    Matilde Malizia

    2011-01-01

    Full Text Available Este trabajo presenta un enfoque teórico-conceptual para el estudio de las urbanizaciones cerradas en ciudades intermedias. En América Latina la investigación urbana fue, en primer lugar, investigación sobre la gr ciudad y los fenómenos resultantes del actual proceso de urbanización. Por lo tanto es escasa la información disponible para el estudio de estos emprendimientos en ciudades de menor tamaño. A través de la bibliografía se puede observar que las ur ciones cerradas adquieren una especificidad propia en las ciudades intermedias. ubican en la periferia de las ciudades, cercanas a espacios verdes con buenas condiciones ambientales y paisajísticas. Impulsan el desarrollo y/o mejora de l infraestructura de servicios. Modifican los procesos de segregación social y fragmentación espacial, acorde a los actuales procesos urbanos. Las barreras físicas se convierten en barreras sociales y marcan profundas diferencias entre quienes viven adent y quienes viven afuera. Finalmente, estas urbanizaciones ocupan superficies pe ueñas en comparación con las extensiones ocupadas por los emprendimientos construidos en las grandes ciudades, situación que, a su vez, limita el tamaño de los espacios ofrecidos.

  2. Factores de riesgo de neumonía nosocomial en terapia intensiva. Hospital “Dr. Ernesto Guevara”

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    Yadira Santiesteban Escalona

    2015-11-01

    Full Text Available Se realizó un estudio analítico, de tipo caso control pareados 1:2, para determinar los factores de riesgo de neumonía nosocomial en pacientes ingresados en terapia intensiva del Hospital “Dr. Ernesto Guevara de la Serna” de Las Tunas, en el período de enero de 2010 a diciembre de 2011. El universo fue de 1035 pacientes ingresados en terapia intensiva (UCI, en dicho período, de ellos constituyeron la muestra, denominada “casos”, 56 pacientes que desarrollaron Neumonía Nosocomial durante su estadía en UCI. Para el “control” de los factores de confusión, por cada caso, se parearon dos pacientes sin diagnóstico de neumonía. La información se obtuvo de fuentes secundarias (historias clínicas. A los casos y a los controles se les llenó una planilla de vaciamiento de datos con las variables analizadas. Los datos se procesaron utilizando el paquete de programas estadísticos Epinfo, versión 6. Para el estudio de los factores de riesgo se realizaron análisis univariados, evaluándose: ODDS RATIO, intervalo de confianza y probabilidad. Resultaron ser factores de riesgo para presentar neumonía nosocomial: la intubación endotraqueal, los trastornos de conciencia, el aislamiento de bacilos no fermentadores, citrobacter diversus y/o klebsiella ssp., haber requerido neurocirugía, el uso de tratamiento antibiótico previo, antiácidos anti H2, nutrición parenteral y la estadía en UCI mayor de siete días.

  3. Terapia comunitária como recurso de abordagem do problema do abuso do álcool, na atenção primária Terapia comunitaria como medio de abordar el problema del abuso de alcohol en la atención primaria Community Therapy as a Method to Address the Problem of Alcohol Abuse in Primary Care

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    Francinete Alves de Oliveira Giffoni

    2011-06-01

    Full Text Available Este estudo teve por objetivo identificar potencialidades da terapia comunitária (TC como recurso de abordagem do problema do abuso do álcool na atenção primária, sob a perspectiva de um usuário. A pesquisa foi realizada na sede do Projeto Quatro Varas, Fortaleza, CE. Empregou-se a abordagem qualitativa de cunho etnográfico, utilizando entrevista semiestruturada e observação participante. Dos 20 participantes da terapia entrevistados, um foi escolhido para entrevista de aprofundamento. Construíram-se dois núcleos temáticos: os efeitos da terapia na abordagem do alcoolismo e terapia como recurso de apoio social, na abordagem dos problemas relacionados ao uso de álcool. Resultados indicam que o diálogo estabelecido na terapia promove ressignificação da problemática e redirecionamento de itinerários terapêuticos, no sentido da gestão da própria vida e busca da cidadania. Conclui-se que a TC favorece a formação de teia de relações sistêmicas, que amplia a compreensão dos problemas decorrentes do uso de álcool, constituindo-se em efetiva estratégia para sua abordagem no campo da saúde comunitária.Este estudio tuvo como objetivo identificar el potencial de la terapia comunitaria (TC como medio de abordar el problema del abuso de alcohol en la atención primaria desde la perspectiva del usuario. La investigación fue realizada en la Sede del Proyecto Cuatro Varas, Fortaleza-CE, Brasil. Fue empleado un abordaje cualitativo de tipo etnográfico, utilizando la entrevista semiestructurada y la observación participante. De los 20 participantes de la terapia entrevistados, uno fue escogido para la entrevista en profundidad. Construimos dos núcleos temáticos: los efectos de la terapia en el abordaje del alcoholismo y la terapia como recurso de apoyo social en el abordaje de los problemas relacionados al uso de alcohol. Los resultados indican que el diálogo establecido en la terapia promueve resignificación de la problem

  4. Expression Profiles of TGF-β and TLR Pathways in Porphyromonas gingivalis and Prevotella intermedia Challenged Osteoblasts.

    Science.gov (United States)

    Aydin, Kubra; Ekinci, Fatma Yesim; Korachi, May

    2015-04-01

    The presence of certain oral pathogens at implant sites can hinder the osseointegration process. However, it is unclear how and by what microorganisms it happens. This study investigated whether the presence of oral pathogens of Porphyromonas gingivalis and Prevotella intermedia individually, play a role in the failure of bone formation by determining the expression profiles of Transforming Growth Factor Beta (TGF-β/Bone Morphogenic Protein (BMP) and Toll-Like Receptor (TLR) pathways in challenged osteoblasts. Cell viability of P. gingivalis and P. intermedia challenged osteoblasts were determined by WST assay. Changes in osteoblast morphology and inhibition of mineralization were observed by Scanning Electron Microscopy (SEM) and Von Kossa staining, respectively. Expression of TGF-β and TLR pathway genes on challenged cells were identified by RT profiler array. Both P. gingivalis and P. intermedia challenges resulted in reduced viability and mineralization of osteoblasts. Viability was reduced to 56.8% (P. gingivalis) and 52.75% (P. intermedia) at 1000 multiplicity. Amongst 48 genes examined, expressions of BMPER, SMAD1, IL8 and NFRKB were found to be highly upregulated by both bacterial challenges (Fold Change > 4). P. gingivalis and P. intermedia could play a role in implant failure by changing the expression profiles of genes related to bone formation and resorption.

  5. ENKAPSULASI DAN STABILITAS PIGMEN KAROTENOID DARI Neurospora intermedia N-1 (Encapsulation and the Stability of Carotenoids from Neurospora intermedia N-1

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    T. Gusdinar

    2011-11-01

    Full Text Available ABSTRAK Neurospora sp merupakan spesies fungi yang dilaporkan menghasilkan pigmen karotenoid, yaitu metabolit sekunder yang termasuk kelompok pigmen yang berwarna kuning, jingga atau merah jingga. Pada penelitian ini telah dilakukan enkapsulasi pigmen karotenoid Neurospora intermedia N-1 menggunakan kopolimer gelatin-maltodekstrin. Suspensi dari campuran ekstrak karotenoid dengan kopolimer gelatin-maltodekstrin, dikeringkan dengan alat spray drier sehingga diperoleh serbuk karotenoid GME (gelatin-maltodekstrin-ekstrak dan diuji stabilitasnya terhadap pengaruh penyimpanan RH 20-30%, selama 5 minggu. Hasil enkapsulasi ekstrak karotenoid diperoleh serbuk GME dengan nilai EY (encapsulation yields ± 48%. Mikroenkapsulasi ekstrak karotenoid tersebut dapat meningkatkan kelarutannya dalam air dan stabil pada kondisi RH 20-30%, stabilitas menurun mulai pada minggu ke 3. Analisis KCKT terhadap serbuk GME menunjukkan penurunan kandungan β-karoten sekitar 30%, setelah penyimpanan 5 minggu. Analisis SEM terhadap serbuk GME menunjukkan partikel yang berbentuk bulat dan berlekuk di permukaan dengan ukuran ±1 µm. ABSTRACT Neurospora sp, a species of fungi was reported containing the carotenoid pigments, the secondary metabolite of the  yellow, orange or red-orange pigments group. On this research, the carotenoid pigments of Neurospora intermedia N-1 has been encapsulated by a copolymer of gelatin-maltodextrins. The encapsulated products were dried by a spray drier and the carotenoid powder was determined its stability to the storage influence at RH 20-30%,  for 5 weeks. Encapsulation product of carotenoids extract obtained the  GME powder with EY value± 48%. Microencapsulation of this extract was increased the water solubility and  stable at RH 20-30% condition,   the stability was decreased start at the third week. HPLC analysis of GME powder showed the decreasing of β-carotene about 30%, after storage for 5 weeks. SEM analysis of GME powder showed

  6. Germinação in vitro de murici-pequeno (Byrsonima intermedia A. Juss. In vitro germination of (Byrsonima intermedia A. Juss.

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    Raírys Cravo Nogueira

    2004-10-01

    Full Text Available O murici-pequeno (Byrsonima intermedia A. Juss., espécie medicinal do Cerrado, apresenta dificuldade de propagação sexuada decorrente da baixa taxa de germinação e emergência lenta das plântulas em campo. Objetivou-se com o presente trabalho estudar aspectos da germinação in vitro de murici-pequeno mediante avaliação do efeito dos meios de cultura MS e WPM e diferentes concentrações de BAP. Frutos maduros foram coletados de populações naturais, dos quais sementes e embriões foram excisados e utilizados como explante. Pelos resultados, infere-se que os meios de cultura mais eficientes para a germinação in vitro de sementes e embriões são o MS e WPM 50%, sem sacarose, apresentando 60% e 100% de germinação, respectivamente. Observou-se também que não se faz necessária a adição de BAP na germinação in vitro de embriões.The murici-pequeno (Byrsonima intermedia A. Juss. is a medicinal plant of cerrado that presents difficulties of sexual propagation due to a low rate of seed germination and plant emergency in the field. The objective of this work was study aspects of the in vitro germination of murici-pequeno evaluating the effects of MS and WPM culture media and different concentrations of BAP. Mature fruits were collected from native plants and their seeds and embryos excised and used as explants. The results indicated that the most effective culture media used for the in vitro seed and embryo germination were MS and WPM 50%, both with no sucrose, presenting 60 and 100% germination, respectively. The use of BAP is not required for in vitro embryo germination.

  7. Terapia hormonal y calidad del hueso Hormone therapy and bone quality

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    2005-08-01

    Full Text Available La osteoporosis se reconoce como uno de los problemas de salud de la población femenina posmenopáusica, y la terapia hormonal de reemplazo (THR como una de las medidas terapéuticas efectivas para evitar la fractura. Nos propusimos mostrar la experiencia acumulada en relación con el efecto de la terapia hormonal de reemplazo sobre la calidad del hueso. En un estudio retrospectivo realizado en 42 mujeres con edades entre 40 y 59 años que asistieron a la Clínica de Climaterio y Osteoporosis y a la consulta multidisciplinaria de climaterio del Hospital Ginecoobstétrico “Ramón González Coro” entre enero de 1997 y diciembre del año 2003, se determinó la calidad ósea mediante absorciometría dual de rayos X en región lumbar (L2-L4 o por ultrasonido del calcáneo (USCAL y recibieron tratamiento continuado con terapia estrogénica (E o con estrógenos progestagenos (EP durante no menos de un año (n = 30. Las mujeres que no pudieron recibir THR fueron agrupadas y evaluadas como grupo control (n =12. Durante el tiempo de observación promedio de 2 años, las mujeres que recibieron THR mejoraron su calidad ósea en el 16,8 %, mientras que las del grupo control empeoraron en el 8 % de los casos. Estos resultados iniciales, aunque son modestos, muestran la utilidad de la THR para mejorar la calidad del hueso y la necesidad de continuar estudios que permitan definir en nuestro medio la persistencia de la mejoría ósea, así como la magnitud de la osteoporosis posmenopáusica.Osteoporosis is recognized as one of the health problems of the female postmenopausic population and the replacement hormone therapy (RHT as one of the effective therapeutical measures to prevent fracture. We proposed ourselves to show the experience accumulated in relation to the effect of the replacement hormone therapy on the bone quality. In a retrospective study conducted among 42 women aged 30-59 that attended the Climacteric and Osteoporosis Clinic and the

  8. Ergonomía y Terapia Ocupacional = Ergonomics and Occupational Therapy

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    Guzman Suárez, Olga Beatriz

    2008-02-01

    Full Text Available RESUMENEn éste artículo se dan a conocer algunas de las aplicaciones más importantes de la ergonomía en Terapia Ocupacional, puesto que a pesar de tener un gran reconocimiento en el sector trabajo, en donde hay un mayor predominio de estudio por parte de la ergonomía, aún se desconocen muchas de las actividades que pueden ser realizadas por éstos profesionales. Terapia Ocupacional abarca el estudio de todas las etapas de la vida del individuo, en cuanto a su desempeño ocupacional se refiere, lo cual garantiza un amplio campo de actuación profesional, que permite profundizar e investigar en diversas áreas, para que desde su experiencia y resultados se facilite el aporte a muchos proyectos de investigación que requieren de conceptos que son transversales a diferentes disciplinas y que pueden constituirse en conocimiento nuevo para la sociedad científica. Con la creación del Grupo de Salud Ocupacional y Ergonomía en la Universidad Manuela Beltrán, se pretende “Desarrollar proyectos de investigación en el campo de la ergonomía, aplicables en los contextos: laboral, escolar, social y familiar; que permitan la adaptación de diferentes entornos al ser humano y a su vez mejorar condiciones tendientes al logro de un óptimo desempeño y productividad”[1]. De esta manera, se inician proyectos de investigación con estudiantes de Terapia Ocupacional, cuyos avances han permitido escribir el presente articulo, se espera además la vinculación de otras áreas para su fortalecimiento. ABSTRACTIn this article they bring themselves to light some of the most important applications of the ergonomics in occupational therapy, since in spite of having a great recognition in the work sector, where there is a greater predominance of study on the part of the ergonomics, still many of the activities they do not know themselves that can be carried out for these professionals. The occupational therapy covers the study of all the phases of the life of

  9. Seguridad de la terapia de interferón alfa 2b recombinante más ribavirina en la hepatitis crónica C

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    Yoan Antonio Sánchez Rodríguez

    2011-03-01

    Full Text Available INTRODUCCIÓN: la hepatitis crónica C ha adquirido rango de pandemia. El virus de la hepatitis C se ha convertido en la causa principal de hepatitis crónica, cirrosis hepática, hepatocarcinoma, y trasplante de hígado a nivel mundial. OBJETIVO: identificar los efectos adversos asociados a la terapia combinada interferón alfa 2b recombinante más ribavirina durante la evolución del tratamiento y hasta 8 semanas después de finalizado, así como los principales efectos asociados a salidas temporales o definitivas de esta terapia. MÉTODOS: estudio de farmacovigilancia cuya serie estuvo conformada por 122 pacientes con hepatitis crónica C atendidos en el Instituto de Gastroenterología desde mayo de 2001 hasta mayo de 2006. Se utilizó interferón alfa 2b recombinante (3 millones de unidades 3 veces por semana más ribavirina (1 000 o 1 200 mg diarios en dependencia del peso corporal durante 48 semanas. RESULTADOS: el 88,5 % del total de casos presentó efectos adversos; de ellos el 79,5 % correspondió al síndrome seudogripal, seguido de manifestaciones hematológicas, neuropsiquiátricas, gastrointestinales, entre otras menos frecuentes. El 6,6 % de la serie presentó salidas temporales del tratamiento por efecto adverso distinto de la anemia y 4 pacientes, salidas definitivas del estudio, tres por anemia hemolítica severa y uno con hipertiroidismo no controlable. CONCLUSIONES: la terapia combinada interferón alfa 2b recombinante más ribavirina resulta segura, donde el mayor número de casos presentó síndrome seudogripal como efecto adverso más frecuente. Las manifestaciones hematológicas asociadas a las salidas definitivas del estudio permitieron recomendar seguimiento estricto de la hemoglobina y profundizar en el diagnóstico y tratamiento de los principales efectos adversos presentes en otros sistemas y asociados a esta terapia.

  10. Aceptación y flexibilidad psicológica ante el contexto docente: un estudio piloto

    OpenAIRE

    Montesinos Marín, Francisco

    2011-01-01

    Se presenta un estudio piloto en el que participaron profesores universitarios dirigido a conocer la utilidad de una intervención orientada a desarrollar competencias para la gestión de emociones y a la clarificación de valores desde la perspectiva de la aceptación psicológica tal como se entiende en la Terapia de Aceptación y Compromiso (Hayes, Strohsal y Wilson, 1999). El estudio permitió identificar emociones y pensamientos que pueden funcionar como barreras psicológicas ...

  11. Efectividad de la terapia floral para el tratamiento de la depresión en pacientes adultos mayores con cáncer

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    Arely Díaz Cifuentes

    2014-08-01

    Full Text Available Se realizó un estudio experimental con el objetivo de determinar la efectividad de la terapia floral en el tratamiento de la depresión leve y moderada, en adultos mayores con cáncer, en el Hospital Provincial Docente de Oncología María Curie, de la provincia de Camagüey, Cuba; el período de estudio comprendió desde enero de 2011 hasta enero de 2012. El universo contempló los pacientes neoplásicos de la tercera edad con síntomas de depresión, remitidos a la consulta de Medicina Tradicional y Natural de la institución y la muestra quedó integrada por 100 pacientes que cumplían los criterios de inclusión y exclusión, los cuales se dividieron en dos grupos de 50 cada uno: el A con tratamiento floral y el B con tratamiento convencional. Se evaluó la persistencia de los síntomas depresivos a los tres y a los seis meses después de aplicado el tratamiento. La terapia floral fue efectiva en el 94% de los casos. Se evidenció la existencia de situaciones intensas antes de ser diagnosticados con cáncer, la más relevante fue la separación familiar, en el 70% de la muestra. Los pacientes del grupo A evolucionaron mejor que los del B. En los primeros, se redujeron notablemente los síntomas depresivos a los tres meses, situación más notable a los seis meses de tratamiento, avalando como efectiva la terapia floral empleada. No se reportaron efectos adversos

  12. Barreiras à implementação da Terapia Compressiva Barreras a la implementación de la Terapia Compresiva Barriers to implementation of Compression Therapy

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    Alda Pereira

    2012-03-01

    Full Text Available Apesar da efetividade da terapia compressiva no tratamento de úlceras venosas ser evidenciada cientificamente, ela não é amplamente usada. O objetivo deste estudo é determinar quais as barreiras/obstáculos à sua implementação e utilização mais ampla. Uma revisão da literatura permitiu definir um conjunto de barreiras potenciais, que foram posteriormente validadas e hierarquizadas segundo peso/importância por peritos em úlcera de perna, num Painel de Delphi online. Depois desenvolveu-se um estudo do tipo transversal descritivo analítico, através da aplicação de questionários construídos para o efeito a uma amostra acidental de 241 profissionais de saúde (enfermeiros e médicos que trabalham em Cuidados de Saúde Primários. Com os dados construímos e validámos a escala de Avaliação de Barreiras à Implementação da Terapia Compressiva na Úlcera Venosa (ABITCUV. Nos resultados destacamos que, relativamente às barreiras que constituem a escala, os profissionais de saúde consideraram, por ordem decrescente de peso/importância: o deficit de formação, o deficit de recursos materiais e financeiros, a ausência de normatização e protocolização de práticas. Depois surgiram a recusa do cliente, falta de motivação e reconhecimento institucional do profissional e resistência do profissional à mudança/novas práticas. Esta hierarquização das barreiras feita pelos profissionais revelou-se similar à sugerida pelos peritos no Painel de Delphi.Pese a que la efectividad de la terapia compresiva en el tratamiento de úlceras venosas sea evidente científicamente, esta no es ampliamente usada. El objetivo de este estudio es determinar cuáles son las barreras/obstáculos a su implementación y utilización más amplia. Una revisión de la literatura permitió definir un conjunto de barreras potenciales, que fueron posteriormente validadas y jerarquizadas según su peso/importancia por expertos en úlcera de pierna, en un

  13. TERAPIA DE INTERACCIÓN PADRES-HIJOS (PCIT

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    Rafael Ferro García

    2014-09-01

    Full Text Available La Terapia de Interacción Padres-Hijos (PCIT es una terapia breve para tratar los problemas de conducta en la infancia. Es un tratamiento que está demostrado válido empíricamente y puede ser considerada como un tratamiento bien establecido para tratar conductas problema en la infancia según las normas de la APA. Este trabajo expone sus características principales, sus componentes clínicos, y los factores que afectan a la efectividad. Hace una revisión de la literatura existente de las investigaciones sobre su efectividad y eficacia. También, se exponen las últimas adaptaciones en diferentes contextos, formatos, problemas y distintas poblaciones.

  14. DANO OCUPACIONAL NA UNIDADE DE TERAPIA INTENSIVA NEONATAL: A PERCEPÇÃO DA ENFERMEIRA

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    MARIA ALVANI DIAS PEDROZA

    2006-01-01

    Full Text Available Se trata de estudio cuantitativo, exploratorio y descriptivo, con el objetivo de conocer la percepción de las enfermeras con respecto a la influencia del desempeño en la asistencia de enfermería y en el ambiente laboral. Tuvo por escenario la Unidad de Terapia Intensiva Neonatal (UTIN de una maternidad escuela, en Fortaleza-CE. Recogimos los datos entre agosto y septiembre de 2004 a través de un cuestionario estructurado en parte, aplicado a 24 enfermeras activas. Verificamos que la mayor concentración de enfermeras (un 37,5% pertenece al grupo de las que ya tienen entre uno a cinco años de trabajo y que 19 (un 79% de ellas poseen otro trabajo, sometidas a una altísima carga horaria. El exceso de trabajo, el estrés y el ruido son los factores que más incomodan en el local, desencadenando el exceso de actividades y cansancio físico. Concluimos que el estrés existente en esta unidad es lo que causa mayor daño a la práctica laboral. El hecho de que esto ocurra con mucha frecuencia nos plantea una reflexión sobre las consecuencias que aporta a la salud del profesional /cuidador, mostrándonos la necesidad de mejorar las condiciones de trabajo.

  15. Bases científicas de la terapia de conducta: nuevas propuestas para un viejo problema

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    Xavier Bornas

    2002-01-01

    Full Text Available En este trabajo se revisa críticamente el recurrente tópico de las bases científicas de la Terapia de Conducta. Asumiendo que la investigación científica de la conducta es conveniente, si no necesaria, para el desarrollo tecnológico y aplicado, se propone tomar en consideración algunos conceptos relativamente nuevos de la Ciencia (por ejemplo, el de caos determinístico y utilizarlos, al menos tentativamente, para procurar obtener un conocimiento mejor de la complejidad de la conducta humana. La incorporación de aquellos conceptos abre una nueva perspectiva complementaria para el estudio de la conducta que se caracteriza por: (a conceder una importancia capital al cambio o flujo continuo de conducta y los procesos de retroalimentación del sistema, (b destacar, además de las relaciones lineales, las de tipo no lineal entre la conducta y el ambiente, y (c tener en cuenta la emergencia de nuevos patrones de comportamiento. Se discute también la necesidad de la investigación de laboratorio y el análisis de los datos así obtenidos.

  16. Treatment with hydroxyurea in thalassemia intermedia with paravertebral pseudotumors of extramedullary hematopoiesis.

    Science.gov (United States)

    Cario, H; Wegener, M; Debatin, K-M; Kohne, E

    2002-08-01

    Excessive ineffective erythropoiesis in thalassemia intermedia may cause paravertebral pseudotumors of extramedullary hematopoiesis. Due to the proximity to the spinal canal, these paravertebral masses carry the risk of severe neurological damage. Treatment strategies include hypertransfusion, radiotherapy, and laminectomy. Hydroxyurea, stimulating fetal hemoglobin synthesis, may represent an alternative therapeutic approach. We report on a 26-year-old patient suffering from thalassemia intermedia with progressive anemia symptoms and presenting multiple intrathoracic paravertebral pseudotumors of extramedullary hematopoiesis. Hypertransfusion therapy and splenectomy were followed by regular transfusion (baseline hemoglobin 10 g/dl) and chelation with desferrioxamine. With this treatment, clinical symptoms disappeared, paravertebral hematopoietic masses did not progress, but severe hemosiderosis developed within a few years. Hydroxyurea therapy was initiated to increase the efficacy of erythropoiesis, thereby reducing the required transfusion volume but suppressing concomitantly further expansion of extramedullary hematopoiesis, and finally leading to a reduction of transfusional iron load. Treatment was started with 4 mg/kg per day and stepwise increased to 12.5 mg/kg per day. The fetal hemoglobin concentration increased from 4.5 to 5.5 g/dl after 1 year and to 9.9 g/dl after 2 years of treatment. The yearly transfusion volume was halved during the 1st year of treatment. At present, after 26 months of treatment, the patient has been transfusion-independent for 10 months. Serum ferritin levels decreased from 2844 to 1335 ng/ml. Size and shape of paravertebral hematopoietic pseudotumors remained stable. No side effects of hydroxyurea have been observed. In thalassemia intermedia patients with extramedullary hematopoiesis, hydroxyurea may lead to independence from regular transfusion therapy without further expansion of ectopic hematopoietic tissue.

  17. Comparative Evaluation of Renal Findings in Beta-Thalassemia Major and Intermedia

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    Ali Derakhshan

    2008-01-01

    Full Text Available Thalassemia is a systematic disease in which the renal involvement has not yet been scrupulously studied. In a cross-sectional study, the renal findings of 50 cases of thalassemia intermedia (group 1 were compared to 58 patients with thalassemia major (group 2. Blood urea nitrogen, serum creatinine, uric acid, calcium, phosphorus, urinalysis, and ultrasonographical findings were evaluated. Mean age was 18 ± 3.0 in group 1 and 17 ± 3.5 years in group 2. The mean of serum ferritin levels was 871 ± 81.8 ng/ml in group 1 vs. 3503 ± 201 ng/ml in thalassemia major (p < 0.05. Ninety-two percent of the patients in group 1 were on hydroxyurea at the time of evaluation. Serum uric acid was significantly higher in group 1 than group 2 patients (5.74 ± 2.95 vs. 4.12 ± 0.9 mg/dl, p < 0.05. Microscopic hematuria (red blood cell in high power field of urine microscopy > 5 was observed among 19 children (17.6%; 17 of them were in group 1. In contrast, children with thalassemia major had significantly higher serum creatinine (0.89 ± 0.18 vs. 0.59 ± 0.37 mg/dl, p < 0.05 and blood urea nitrogen values (12.14 ± 5.58 vs. 13.85 ± 3.54 mg/dl, p < 0.05. We conclude that significant renal involvement is not a frequent complication in children and young adults suffering from thalassemia. Hyperuricemia and microscopic hematuria are more common in thalassemia intermedia than thalassemia major. Microscopic hematuria in thalassemia intermedia might be related to either hypercalciuria or hyperuricosuria.

  18. Prevalence, risk factors and clinical signs predictive for equine pituitary pars intermedia dysfunction in aged horses.

    Science.gov (United States)

    McGowan, T W; Pinchbeck, G P; McGowan, C M

    2013-01-01

    Equine pituitary pars intermedia dysfunction (PPID) is an ageing-related neurodegenerative disorder. The prevalence and risk factors for PPID using seasonally adjusted basal adrenocorticotropic hormone (ACTH) concentrations in aged horses have not been previously reported. To determine the prevalence, risk factors and clinical signs predictive for PPID in a population of horses aged ≥ 15 years in Queensland, Australia. Owner-reported data was obtained using a postal questionnaire distributed to an equestrian group. A subgroup of surveyed owners were visited and a veterinary physical examination performed on all horses aged ≥ 15 years. Blood samples were analysed for basal plasma alpha melanocyte-stimulating hormone (α-MSH) and ACTH concentrations, routine haematology and selected biochemistry. Aged horses with elevations above seasonally adjusted cut-off values for basal plasma ACTH were considered positive for PPID. Positive horses were compared with their aged counterparts to determine risk factors and clinical signs associated with PPID. Pituitary pars intermedia dysfunction was prevalent in aged horses (21.2%) despite owners infrequently reporting it as a known or diagnosed disease or disorder. Numerous clinical or historical signs were associated with an increased risk of PPID in the univariable model, but only age (odds ratio (OR) 1.18; 95% confidence interval (CI) 1.11-1.25, P<0.001) and owner-reported history of hirsutism (OR 7.80; 95% CI 3.67-16.57, P<0.001) remained in the final multivariable model. There were no routine haematological or biochemical variables supportive of a diagnosis of PPID. Pituitary pars intermedia dysfunction occurs commonly in aged horses despite under-recognition by owners. The increased risk of PPID with age supports that this is an ageing associated condition. Aged horses with clinical or historical signs consistent with PPID, especially owner-reported hirsutism (delayed shedding and/or long hair coat), should be tested and

  19. Prevotella intermedia infection causing acute and complicated aortitis-A case report.

    Science.gov (United States)

    Boersma, C; Kampschreur, L M; Buter, H; Doorenbos, B M; Klinkert, P; Koning, G G

    2017-01-01

    Aortitis is a general term that refers to all conditions involving an inflammation of the aortic wall. This case report describes the surgical approach of a patient with infectious and symptomatic aortitis caused by the rare vector Prevotella intermedia. A 44-year old male patient was admitted with fever and general discomfort after a period of sore throat in a non-teaching hospital. After two weeks he developed acute abdominal and back pain accompanied by sweating and elevated infection parameters. Computed tomography angiography revealed atherosclerotic changes of the infrarenal aorta with a locally contained rupture of the aorta alongside peri-aortal signs of inflammation (and aortitis aspects). An urgent aortic reconstruction was performed according to Nevelsteen. The blood cultures turned out positive for Prevotella intermedia. Postoperatively the patient received antibiotics for six weeks. The patient recovered uneventful from this infection and surgical procedure. A complicated and acute aortitis is a rare but potentially life-threatening disease. The aetiology can be ordered into two main groups; inflammatory and infectious. Diagnosis is based upon symptoms, biochemical values, microbiological results and imaging modalities. Treatment depends on aetiology and should be discussed in an experienced multidisciplinary setting. Infectious aortitis should be treated with antibiotics for at least six weeks with close monitoring of the patient's clinic and biochemical values, even after surgery. Prevotella intermedia is a rare causative agent for aortitis. Acute aortitis is a challenging clinical entity which should be managed in an equipped medical center by an experienced multidisciplinary team. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  20. Transcriptome changes associated with anaerobic growth in Yersinia intermedia (ATCC29909.

    Directory of Open Access Journals (Sweden)

    Lavanya Babujee

    Full Text Available The yersiniae (Enterobacteriaceae occupy a variety of niches, including some in human and flea hosts. Metabolic adaptations of the yersiniae, which contribute to their success in these specialized environments, remain largely unknown. We report results of an investigation of the transcriptome under aerobic and anaerobic conditions for Y. intermedia, a non-pathogenic member of the genus that has been used as a research surrogate for Y. pestis. Y. intermedia shares characteristics of pathogenic yersiniae, but is not known to cause disease in humans. Oxygen restriction is an important environmental stimulus experienced by many bacteria during their life-cycles and greatly influences their survival in specific environments. How oxygen availability affects physiology in the yersiniae is of importance in their life cycles but has not been extensively characterized.Tiled oligonucleotide arrays based on a draft genome sequence of Y. intermedia were used in transcript profiling experiments to identify genes that change expression in response to oxygen availability during growth in minimal media with glucose. The expression of more than 400 genes, constituting about 10% of the genome, was significantly altered due to oxygen-limitation in early log phase under these conditions. Broad functional categorization indicated that, in addition to genes involved in central metabolism, genes involved in adaptation to stress and genes likely involved with host interactions were affected by oxygen-availability. Notable among these, were genes encoding functions for motility, chemotaxis and biosynthesis of cobalamin, which were up-regulated and those for iron/heme utilization, methionine metabolism and urease, which were down-regulated.This is the first transcriptome analysis of a non-pathogenic Yersinia spp. and one of few elucidating the global response to oxygen limitation for any of the yersiniae. Thus this study lays the foundation for further experimental

  1. Novos conceitos em cuidados paliativos na unidade de terapia intensiva

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    Cristina Bueno Terzi Coelho

    Full Text Available RESUMO Alguns dos pacientes admitidos em uma unidade de terapia intensiva podem enfrentar condições de doença terminal, que geralmente levam à morte. O conhecimento sobre cuidados paliativos é recomendado para os profissionais de saúde encarregados do cuidado destes pacientes. Em muitas situações, os pacientes devem ser avaliados diariamente, já que a introdução de novos tratamentos pode ou não ser benéfica para eles. As discussões entre os membros da equipe de saúde, relacionadas ao prognóstico e aos objetivos do tratamento, devem ser avaliadas cuidadosamente em cooperação com os pacientes e seus familiares. A adoção na unidade de terapia intensiva de protocolos relacionados a pacientes em final da vida é fundamental. É importante ter uma equipe multidisciplinar para determinar se é necessário deixar de iniciar ou mesmo retirar tratamentos avançados. Além disto, pacientes e familiares devem ser informados de que os cuidados paliativos envolvem o melhor tratamento possível para aquela situação específica, assim como respeitar suas vontades e considerar as bases sociais e espirituais dos mesmos. Assim, o objetivo desta revisão foi apresentar os cuidados paliativos como uma opção razoável para dar suporte à equipe da unidade de terapia intensiva na assistência a pacientes com doença terminal. São apresentadas atualizações com relação a dieta, ventilação mecânica e diálise nestes pacientes. Ainda, discutiremos o programa, comum nos Estados Unidos, conhecido como filosofia hospice, como alternativa ao ambiente da unidade de terapia intensiva/hospital.

  2. Double heterozygocity for hemoglobin C and beta thalassemia dominant: A rare case of thalassemia intermedia

    Directory of Open Access Journals (Sweden)

    Alexandra Agapidou

    2018-01-01

    Full Text Available Beta thalassemia dominant results from mutations in the β globin chain gene resulting in the production of elongated, highly unstable beta globin chains. Several such mutations have been described and in a heterozygous state they may confer a phenotype more severe than that of β thalassemia trait and lead to a clinical syndrome of thalassemia intermedia and its associated complications such as extramedullary hemopoiesis, bone disease, endocrinopathies and iron overload even in the absence of transfusion. In this report we present a case of double heterozygocity for HbC and β thalassemia dominant leading to a series of complications that were treated successfully once the correct diagnosis was made.

  3. Intermedia transfer factors for fifteen toxic pollutants released to air basins in California

    Energy Technology Data Exchange (ETDEWEB)

    McKone, T.E.; Daniels, J.I. [Lawrence Livermore National Lab., CA (United States); Chiao, F.F.; Hsieh, D.P.H. [Univ. of California, Davis, CA (United States)

    1993-10-01

    This report provides a summary definition of the intermedia-transfer factors (ITFs). Methods are discussed for estimating these parameters in the absence of measured values, and the estimation errors inherent in these estimation methods are considered. A detailed summary is provided of measured and estimated ITF values for fifteen air contaminants. They include: 1,3 butadiene; cadmium; cellosolve; cellosolve acetate; chloroform; di-2-ethylhexylphthalate; 1,4-dioxame; hexachlorobenzene; inorganic arsenic; inorganic lead; nickel; tetrachloroethylene; toluene; toluene-2,4-diisocyanate; and 1,3-xylene. Recommendations are made regarding the expected value and variance in these values for use in exposure models.

  4. Composition, Cytotoxic and Antimicrobial Activities of Satureja intermedia C.A.Mey Essential Oil

    OpenAIRE

    Sharifi-Rad, Javad; Sharifi-Rad, Mehdi; Hoseini-Alfatemi, Seyedeh; Iriti, Marcello; Sharifi-Rad, Majid; Sharifi-Rad, Marzieh

    2015-01-01

    In this study, the essential oil (EO) constituents from the aerial parts of Satureja intermedia C.A.Mey were detected by GC and GC/MS. The antimicrobial activity of EO on oral pathogens and its cytotoxicity to human cancer cells were determined by the microbroth dilution method and the crystal violet staining method, respectively. Thirty-nine compounds were identified and the main EO constituents were γ-terpinene (37.1%), thymol (30.2%), p-cymene (16.2%), limonene (3.9%), α-terpinene (3.3%...

  5. Gene expression profile and pathogenicity of biofilm-forming Prevotella intermedia strain 17.

    Science.gov (United States)

    Yamanaka, Takeshi; Furukawa, Tomoyo; Matsumoto-Mashimo, Chiho; Yamane, Kazuyoshi; Sugimori, Chieko; Nambu, Takayuki; Mori, Naoki; Nishikawa, Hiroyuki; Walker, Clay B; Leung, Kai-Poon; Fukushima, Hisanori

    2009-01-16

    Prevotella intermedia (P. intermedia), a gram-negative, black-pigmented anaerobic rod, has been implicated in the development of chronic oral infection. P. intermedia strain 17 was isolated from a chronic periodontitis lesion in our laboratory and described as a viscous material producing strain. The stock cultures of this strain still maintain the ability to produce large amounts of viscous materials in the spent culture media and form biofilm-like structures. Chemical analyses of this viscous material showed that they were mainly composed of neutral sugars with mannose constituting 83% of the polysaccharides. To examine the biological effect of the extracellular viscous materials, we identified and obtained a naturally-occurring variant strain that lacked the ability to produce viscous materials in vitro from our stock culture collections of strain 17, designated as 17-2. We compared these two strains (strains 17 versus 17-2) in terms of their capacities to form biofilms and to induce abscess formation in mice as an indication of their pathogenicity. Further, gene expression profiles between these two strains in planktonic condition and gene expression patterns of strain 17 in solid and liquid cultures were also compared using microarray assays. Strain 17 induced greater abscess formation in mice as compared to that of the variant. Strain 17, but not 17-2 showed an ability to interfere with the phagocytic activity of human neutrophils. Expression of several genes which including those for heat shock proteins (DnaJ, DnaK, ClpB, GroEL and GroES) were up-regulated two to four-fold with statistical significance in biofilm-forming strain 17 as compared to the variant strain 17-2. Strain 17 in solid culture condition exhibited more than eight-fold up-regulated expression levels of several genes which including those for levanase, extracytoplasmic function-subfamily sigma factor (sigmaE; putative) and polysialic acid transport protein (KpsD), as compared to those of

  6. Gene expression profile and pathogenicity of biofilm-forming Prevotella intermedia strain 17

    Directory of Open Access Journals (Sweden)

    Mori Naoki

    2009-01-01

    Full Text Available Abstract Background Prevotella intermedia (P. intermedia, a gram-negative, black-pigmented anaerobic rod, has been implicated in the development of chronic oral infection. P. intermedia strain 17 was isolated from a chronic periodontitis lesion in our laboratory and described as a viscous material producing strain. The stock cultures of this strain still maintain the ability to produce large amounts of viscous materials in the spent culture media and form biofilm-like structures. Chemical analyses of this viscous material showed that they were mainly composed of neutral sugars with mannose constituting 83% of the polysaccharides. To examine the biological effect of the extracellular viscous materials, we identified and obtained a naturally-occurring variant strain that lacked the ability to produce viscous materials in vitro from our stock culture collections of strain 17, designated as 17-2. We compared these two strains (strains 17 versus 17-2 in terms of their capacities to form biofilms and to induce abscess formation in mice as an indication of their pathogenicity. Further, gene expression profiles between these two strains in planktonic condition and gene expression patterns of strain 17 in solid and liquid cultures were also compared using microarray assays. Results Strain 17 induced greater abscess formation in mice as compared to that of the variant. Strain 17, but not 17-2 showed an ability to interfere with the phagocytic activity of human neutrophils. Expression of several genes which including those for heat shock proteins (DnaJ, DnaK, ClpB, GroEL and GroES were up-regulated two to four-fold with statistical significance in biofilm-forming strain 17 as compared to the variant strain 17-2. Strain 17 in solid culture condition exhibited more than eight-fold up-regulated expression levels of several genes which including those for levanase, extracytoplasmic function-subfamily sigma factor (σE; putative and polysialic acid transport

  7. PET CT imaging in extramedullary hematopoiesis and lung cancer surprise in a case with thalassemia intermedia

    Directory of Open Access Journals (Sweden)

    Semra Paydaş

    2011-03-01

    Full Text Available Extramedullary hematopoiesis (EMH is the production of hematopoietic precursors outside the bone marrow cavity, and it causes mass effects according to its localization. Magnetic resonance imaging (MRI and/or computed tomography (CT scans are used most commonly to detect EMH foci. We report herein a case with thalassemia intermedia causing paravertebral mass associated with EMH detected by CT scan. We further evaluated the case with positron emission tomography (PET CT, and lung cancer, which was not revealed in the CT scan, was detected coincidentally.

  8. Los límites de la ciudad intermedia : conectividad e innovación

    OpenAIRE

    Basurto Alvarez, Rodolfo

    2014-01-01

    Este trabajo vincula conectividad e innovación para fijar los alcances de la segunda modernidad de una ciudad intermedia (Hermosillo, Sonora, México), estableciendo sus restricciones para ascender como nodo de comunicación sur-norte de la ciudad global. Se evalúa la diversidad incipiente del ecosistema urbano mediante el referente hipotético de la existencia de flujos comunicacionales de una sola dirección (norte-sur) en el funcionamiento de las empresas multinacionales. ...

  9. Description of the agonistic behaviors of Loxosceles laeta, L. hirsuta, and L. intermedia (Araneae: Sicariidae)

    OpenAIRE

    Fischer, Marta L.

    2008-01-01

    Os grupos de radiação evolutiva de Loxosceles Heinecken & Lowe, 1835 além de compartilharem caracteres morfológicos aparentemente também apresentam comportamentos semelhantes. Assim, o presente estudo objetivou descrever o comportamento agonístico dos machos das espécies do grupo spadicea - Loxosceles intermedia Mello-Leitão, 1934 e Loxosceles hirsuta Mello-Leitão, 1931 - e laeta -Loxosceles laeta (Nicolet 1849) - ocorrentes no estado do Paraná. Para tal, os machos foram pareados, sendo o inv...

  10. Uso de terapias integrativas e complementares por pacientes em quimioterapia

    Directory of Open Access Journals (Sweden)

    Julyane Felipette Lima

    2015-09-01

    Full Text Available Objetivo: Conhecer as terapias integrativas e complementares utilizadas pelos pacientes em quimioterapia oncológica. Metodologia: Estudo qualitativo, realizado com seis pacientes oncológicos em tratamento quimioterápico, em um serviço de oncologia do sul do Rio Grande do Sul, Brasil. Os dados foram coletados por meio de entrevista, em junho de 2010, sendo a análise de conteúdo tipo temática. Resultados: As terapias apontadas neste estudo foram a homeopatia, a fitoterapia e as plantas medicinais e percebeu-se que elas aumentam a sensação de bem-estar, possibilitam o estabelecimento de vínculos positivos com profissionais da saúde, e fazem parte do saber popular e estão interligadas ao conhecimento científico. Conclusões: Apreende-se que uso de terapias integrativas e complementares em oncologia permite a aproximação do profissional enfermeiro com o paciente em quimioterapia, conhecendo-o em sua complexidade.

  11. Terapia comportamental cognitiva: uma comparação entre perspectivas

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    Rachel Rodrigues Kerbauy

    Full Text Available A Terapia Comportamental Cognitiva desenvolveu-se a partir de dados e maneiras de trabalhar da Terapia Cognitiva e da Terapia Comportamental. Essas origens e as várias influências parecem determinar maneiras diferentes de conduzir a terapia o que se procurou analisar neste trabalho. Optou-se pela apresentação do trabalho de três autores: Ellis, Beck e Meichembaum como representativos da área embora se reconheça que há diferenças entre algumas concepções e também unanimidade na explicação de comportamentos, especialmente emocionais.

  12. Células madre: problemas biológicos que dificultan la terapia celular

    OpenAIRE

    Zapata, Agustín

    2015-01-01

    La terapia celular es una terapia avanzada en la que las células pueden ser usadas como auténticos medicamentos. Como elemento esencial de la medicina regenerativa, el objetivo principal de estas terapias es la sustitución de tejidos dañados o deficientes por otros sanos o funcionales Las células madre (células stem), ya sean estas embrionarias, pluripotentes inducidas o adultas, son una fuente potencialmente ilimitada de células para la investigación o para su uso clínico en terapia celul...

  13. Varianti genetiche associate al rimodellamento inverso dopo terapia di resincronizzazione cardiaca

    OpenAIRE

    De Maria, Renata; Lunati, M.; Landolina, M.; Gasparini, M.; Schmitz, Boris; Sanzo, A.; Galimberti, P.; Campolo, J.; Parolini, M.; Brand, S.M.; Parodi, O.

    2011-01-01

    In pazienti con scompenso cardiaco cronico sistolico (HF) la terapia di resincronizzazione cardiaca (CRT) determina effetti favorevoli sulle dimensioni e la funzione ventricolare noti come rimodellamento inverso (RR).

  14. Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia.

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    Natalia M Tavares

    Full Text Available BACKGROUND: Leishmania transmission occurs in the presence of insect saliva. Immunity to Phlebotomus papatasi or Lutzomyia longipalpis saliva or salivary components confers protection against an infection by Leishmania in the presence of the homologous saliva. However, immunization with Lutzomyia intermedia saliva did not protect mice against Leishmania braziliensis plus Lu. intermedia saliva. In the present study, we have studied whether the immunization with Lu. longipalpis saliva or a DNA plasmid coding for LJM19 salivary protein would be protective against L. braziliensis infection in the presence of Lu. intermedia saliva, the natural vector for L. braziliensis. METHODOLOGY/PRINCIPAL FINDINGS: Immunization with Lu. longipalpis saliva or with LJM19 DNA plasmid induced a Delayed-Type Hypersensitivity (DTH response against Lu. longipalpis as well as against a Lu. intermedia saliva challenge. Immunized and unimmunized control hamsters were then intradermally infected in the ears with L. braziliensis in the presence of Lu. longipalpis or Lu. intermedia saliva. Animals immunized with Lu. longipalpis saliva exhibited smaller lesion sizes as well as reduced disease burdens both at lesion site and in the draining lymph nodes. These alterations were associated with a significant decrease in the expression levels of IL-10 and TGF-β. Animals immunized with LJM19 DNA plasmid presented similar findings in protection and immune response and additionally increased IFN-γ expression. CONCLUSIONS/SIGNIFICANCE: Immunization with Lu. longipalpis saliva or with a DNA plasmid coding LJM19 salivary protein induced protection in hamsters challenged with L. braziliensis plus Lu. intermedia saliva. These findings point out an important role of immune response against saliva components, suggesting the possibility to develop a vaccine using a single component of Lu. longipalpis saliva to generate protection against different species of Leishmania, even those

  15. Population-Genomic Insights into Variation in Prevotella intermedia and Prevotella nigrescens Isolates and Its Association with Periodontal Disease

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    Yifei Zhang

    2017-09-01

    Full Text Available High-throughput sequencing has helped to reveal the close relationship between Prevotella and periodontal disease, but the roles of subspecies diversity and genomic variation within this genus in periodontal diseases still need to be investigated. We performed a comparative genome analysis of 48 Prevotella intermedia and Prevotella nigrescens isolates that from the same cohort of subjects to identify the main drivers of their pathogenicity and adaptation to different environments. The comparisons were done between two species and between disease and health based on pooled sequences. The results showed that both P. intermedia and P. nigrescens have highly dynamic genomes and can take up various exogenous factors through horizontal gene transfer. The major differences between disease-derived and health-derived samples of P. intermedia and P. nigrescens were factors related to genome modification and recombination, indicating that the Prevotella isolates from disease sites may be more capable of genomic reconstruction. We also identified genetic elements specific to each sample, and found that disease groups had more unique virulence factors related to capsule and lipopolysaccharide synthesis, secretion systems, proteinases, and toxins, suggesting that strains from disease sites may have more specific virulence, particularly for P. intermedia. The differentially represented pathways between samples from disease and health were related to energy metabolism, carbohydrate and lipid metabolism, and amino acid metabolism, consistent with data from the whole subgingival microbiome in periodontal disease and health. Disease-derived samples had gained or lost several metabolic genes compared to healthy-derived samples, which could be linked with the difference in virulence performance between diseased and healthy sample groups. Our findings suggest that P. intermedia and P. nigrescens may serve as “crucial substances” in subgingival plaque, which may

  16. Population-Genomic Insights into Variation in Prevotella intermedia and Prevotella nigrescens Isolates and Its Association with Periodontal Disease

    Science.gov (United States)

    Zhang, Yifei; Zhen, Min; Zhan, Yalin; Song, Yeqing; Zhang, Qian; Wang, Jinfeng

    2017-01-01

    High-throughput sequencing has helped to reveal the close relationship between Prevotella and periodontal disease, but the roles of subspecies diversity and genomic variation within this genus in periodontal diseases still need to be investigated. We performed a comparative genome analysis of 48 Prevotella intermedia and Prevotella nigrescens isolates that from the same cohort of subjects to identify the main drivers of their pathogenicity and adaptation to different environments. The comparisons were done between two species and between disease and health based on pooled sequences. The results showed that both P. intermedia and P. nigrescens have highly dynamic genomes and can take up various exogenous factors through horizontal gene transfer. The major differences between disease-derived and health-derived samples of P. intermedia and P. nigrescens were factors related to genome modification and recombination, indicating that the Prevotella isolates from disease sites may be more capable of genomic reconstruction. We also identified genetic elements specific to each sample, and found that disease groups had more unique virulence factors related to capsule and lipopolysaccharide synthesis, secretion systems, proteinases, and toxins, suggesting that strains from disease sites may have more specific virulence, particularly for P. intermedia. The differentially represented pathways between samples from disease and health were related to energy metabolism, carbohydrate and lipid metabolism, and amino acid metabolism, consistent with data from the whole subgingival microbiome in periodontal disease and health. Disease-derived samples had gained or lost several metabolic genes compared to healthy-derived samples, which could be linked with the difference in virulence performance between diseased and healthy sample groups. Our findings suggest that P. intermedia and P. nigrescens may serve as “crucial substances” in subgingival plaque, which may reflect changes in

  17. A terapia comunitária em um centro de atenção psicossocial: (desatando pontos relevantes La terapia comunitaria en los centros de atención psicosocial: (desatando nudos relevantes Community therapy at psychosocial care centers: (disconnecting relevant points

    Directory of Open Access Journals (Sweden)

    Maria de Oliveira Ferreira Filha

    2010-06-01

    Full Text Available Este estudo objetiva descrever a implantação da Terapia Comunitária (TC em um Centro de Atenção Psicossocial (CAPS, identificar problemas vivenciados por usuários e familiares e estratégias de enfrentamento, além de analisar a contribuição da Terapia Comunitária para inclusão social do usuário. Consiste num estudo predominantemente qualitativo desenvolvido em um Centro de Atenção Psicossocial. O material foi produzido em 12 rodas de Terapia tendo sido utilizadas a técnica de entrevista semi-estruturada e as fichas de avaliação da TC. Os resultados evidenciaram como problemas principais: conflitos familiares, insônia e abandono. Várias estratégias de enfrentamento foram mencionadas: apoio familiar, crença religiosa e ajuda profissional. Comprovou-se que a participação dos usuários tem contribuído para a inclusão social destes ao relatarem mudanças de comportamento nos relacionamentos interpessoais. A experiência tem mostrado ótimos resultados, pois o estudo trabalha uma abordagem que contempla aspectos sociais ainda negligenciados.Este estudio tiene por objetivo describir la implantación de la Terapia Comunitaria (TC en un Centro de Atención Psico-social (CAPS, identificar problemas vivenciados por los usuarios y sus familiares y las estrategias de enfrentamiento, además de analizar la contribución de la Terapia Comunitaria para la inclusión social del usuario. Es un estudio predominantemente cualitativo desarrollado en un Centro de Atención Psico-social. El material fue elaborado a partir de la realización de 12 ruedas de Terapia, y se utilizaron la técnica de entrevista semi-estructurada y las fichas de evaluación de la Terapia Comunitaria. Los resultados evidenciaron problemas como: conflictos familiares, insomnio y abandono. Se mencionaron varias estrategias de enfrentamiento: apoyo familiar, fe religiosa, ayuda profesional. Se comprobó que la participación de los usuarios contribuyó para la inclusi

  18. Spinal cord compression due to extramedullary hematopoiesis in beta-thalassemia intermedia

    International Nuclear Information System (INIS)

    Munn, Rita K.; Kramer, Carol A.; Arnold, Susanne M.

    1998-01-01

    Background: Extramedullary hematopoiesis (EMH) occurs in many disorders, including thalassemias and other hemoglobinopathies, and commonly presents in the spleen and liver. We present a case of spinal cord compression in a patient with beta-thalassemia intermedia, and review the literature and available treatment options. Patient and Methods: A 35-year-old black female with beta-thalassemia intermedia presented with a 3-week history of back pain and lower extremity weakness. Neurologic examination was consistent with spinal cord compression, and gadolinium enhanced magnetic resonance imaging (MRI) confirmed this diagnosis. She was given intravenous steroids and radiotherapy was begun in 200 cGy fractions to a total dose of 2000 cGy. Results: At the completion of radiotherapy the patient was ambulatory with mild residual weakness. MRI scans 16 months later showed smaller, but persistent masses, and she remains asymptomatic 5 years from her diagnosis. Conclusion: Recognition of spinal cord EMH requires prompt physical examination and MRI for accurate diagnosis. EMH can be managed with radiation, surgery, transfusions, or a combination of these therapies. Radiation in conservative doses of (750-3500 cGy) is non-invasive, avoids the surgical risks of potentially severe hemorrhage and incomplete resection, and has a high complete remission rate in the majority of patients. Relapse rates are moderate (37.5%), but retreatment provides excellent chance for second remission

  19. Noninvasive analysis of skin iron and zinc levels in beta-thalassemia major and intermedia

    International Nuclear Information System (INIS)

    Gorodetsky, R.; Goldfarb, A.; Dagan, I.; Rachmilewitz, E.A.

    1985-01-01

    Diagnostic x-ray spectrometry, a method based on x-ray fluorescence analysis, was used for noninvasive determination of iron and zinc in two distinct skin areas, representing predominantly dermal and epidermal tissues, in 56 patients with beta-thalassemia major and intermedia. The mean iron levels in the skin of patients with beta-thalassemia major and intermedia were elevated by greater than 200% and greater than 50%, respectively, compared with control values. The zinc levels of both skin areas examined were within the normal range. The data indicate that the rate and number of blood transfusions, which correlated well with serum ferritin levels (r . 0.8), are not the only factors that determine the amount of iron deposition in the skin (r less than 0.6). Other sources of iron intake contribute to the total iron load in the tissues, particularly in patients who are not given multiple transfusions. The noninvasive quantitation of skin levels may reflect the extent of iron deposition in major parenchymal organs. Repeated DXS examinations of the skin could monitor the clearance of iron from the tissues of patients with iron overload in the course of therapy with chelating agents

  20. Breakdown of albumin and haemalbumin by the cysteine protease interpain A, an albuminase of Prevotella intermedia.

    Science.gov (United States)

    Byrne, Dominic P; Manandhar, Surya P; Potempa, Jan; Smalley, John W

    2015-09-24

    Prevotella intermedia is a Gram-negative black-pigmenting oral anaerobe associated with periodontitis in humans, and has a haem requirement for growth, survival and virulence. It produces an iron porphyrin-containing pigment comprising monomeric iron (III) protoporphyrin IX (Fe(III)PPIX.OH; haematin). The bacterium expresses a 90-kDa cysteine protease termed interpain A (InpA) which both oxidizes and subsequently degrades haemoglobin, releasing haem. However, it is not known whether the enzyme may play a role in degrading other haem-carrying plasma proteins present in the gingival sulcus or periodontal pocket from which to derive haem. This study evaluated the ability of InpA to degrade apo- and haem-complexed albumin. Albumin breakdown was examined over a range of pH and in the presence of reducing agent; conditions which prevail in sub- and supra-gingival plaque. InpA digested haemalbumin more efficiently than apoalbumin, especially under reducing conditions at pH 7.5. Under these conditions InpA was able to substantially degrade the albumin component of whole human plasma. The data point to InpA as an efficient "albuminase" with the ability to degrade the minor fraction of haem-bound albumin in plasma. InpA may thus contribute significantly to haem acquisition by P. intermedia under conditions of low redox potential and higher pH in the inflamed gingival crevice and diseased periodontal pocket where haem availability is tightly controlled by the host.

  1. Mutagenic evaluation and chemical investigation of Byrsonima intermedia A. Juss. leaf extracts.

    Science.gov (United States)

    Sannomiya, Miriam; Cardoso, Cássia R P; Figueiredo, Maria E; Rodrigues, Clenilson M; dos Santos, Lourdes C; dos Santos, Fabio V; Serpeloni, Juliana M; Cólus, Ilce M S; Vilegas, Wagner; Varanda, Eliana A

    2007-06-13

    Byrsonima intermedia is a native species of the cerrado formation (tropical American savannah). In Brazil, this plant has been used for the treatment of fever, in ulcers, as a diuretic, as antiasthmatics and in skin infections. Members of the genus Byrsonima (Malpighiaceae) are employed not only in the folk medicine but also as food to make juice, jellies and liquor. The aim of this work was to evaluate the mutagenic effects of Byrsonima intermedia, common name 'murici'. Phytochemical analysis of methanol extract furnished (+)-catechin, (-)-epicatechin, quercetin-3-O-beta-d-galactopyranoside, methyl gallate, gallic acid, quercetin-3-O-alpha-l-arabinopyranoside, amentoflavone, quercetin, quercetin-3-O-(2''-O-galloyl)-beta-galactopyranoside and quercetin-3-O-(2''-O-galloyl)-alpha-arabinopyranoside. Methanol, hydromethanol and chloroform extracts were evaluated in mutagenic assay with Salmonella typhimurium (Ames test) and mice (Micronucleus test). The methanolic extract presented signs of mutagenic activity for the strains TA98 and TA100 in the Ames assay. Mutagenicity was not observed in vivo.

  2. Microhabitats occupied by Loxosceles intermedia and Loxosceles laeta (Araneae: Sicariidae) in Curitiba, Paraná, Brazil.

    Science.gov (United States)

    Fischer, Marta Luciane; Vasconcellos-Neto, João

    2005-09-01

    A survey was done of the environments and substrata occupied by Loxosceles intermedia Mello-Leitão, 1934 and Loxosceles laeta (Nicolet, 1849) (Araneae: Sicariidae) in urban woods and anthropic constructions in Curitiba. In total, 2,099 trees, 364 decaying logs as well as stones and roots, built-up areas, and wasteland in six urban parks and 60 residences were inspected. In total, 1,775 m2 of vegetation was inspected, but spiders and their vestiges were collected only in and around buildings in urban parks and residences. L. intermedia was more common than L. laeta and occurred both indoors and outdoors, whereas L. laeta was more common indoors in wooden houses. The two species did not occur in the same microhabitats, although both preferred paper, wood, and construction materials. Spiders collected in urban parks were heavier than those collected in residences, although only males collected in urban parks were larger than those from residences. The lack of vestiges indicative of a previous occupation in the urban parks suggested that both species occupied primarily the anthropic environment where they found numerous substrata that offered thermal isolation and suitable conditions for web fixation, ecdysis, and reproduction.

  3. Kinetic and mechanistic characterization of the Sphingomyelinases D from Loxosceles intermedia spider venom.

    Science.gov (United States)

    de Andrade, Sonia A; Murakami, Mario T; Cavalcante, Danielle P; Arni, Raghuvir K; Tambourgi, Denise V

    2006-03-15

    Envenomation by arachnids of the genus Loxosceles leads to local dermonecrosis and serious systemic toxicity mainly induced by sphingomyelinases D (SMase D). These enzymes catalyze the hydrolysis of sphingomyelin resulting in the formation of ceramide-phosphate and choline as well as the cleavage of lysophosphatidyl choline generating the lipid mediator lysophosphatidic acid. We have, previously, cloned and expressed two functional SMase D isoforms, named P1 and P2, from Loxosceles intermedia venom and comparative protein sequence analysis revealed that they are highly homologous to SMase I from Loxosceles laeta which folds to form an (alpha/beta)8 barrel. In order to further characterize these proteins, pH dependence kinetic experiments and chemical modification of the two active SMases D isoforms were performed. We show here that the amino acids involved in catalysis and in the metal ion binding sites are strictly conserved in the SMase D isoforms from L. intermedia. However, the kinetic studies indicate that SMase P1 hydrolyzes sphingomyelin less efficiently than P2, which can be attributed to a substitution at position 203 (Pro-Leu) and local amino acid substitutions in the hydrophobic channel that could probably play a role in the substrate recognition and binding.

  4. Terapia cognitivo-comportamental da fobia social: modelos e técnicas

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    Gustavo J. Fonseca D'El Rey

    2006-08-01

    Full Text Available A fobia social é um dos transtornos mentais mais prevalentes na população geral. As principais formas de tratamento são a psicoterapia e a farmacoterapia. Este artigo tem como objetivo realizar uma breve revisão bibliográfica dos mais importantes modelos e técnicas cognitivas e comportamentais da fobia social. Os modelos antigos - modelo de déficit de habilidades sociais, modelo de crenças irracionais, modelo da vulnerabilidade cognitiva e os modelos integrativos - serão revisados brevemente. Serão apresentadas as técnicas de tratamento - como a exposição, reestruturação cognitiva, técnicas de relaxamento e treino de habilidades sociais. Estudos de metaanálise sobre a eficácia da terapia cognitivo-comportamental no tratamento da fobia social também serão descritos.La fobia social es uno de los trastornos mentales más prevalentes en la población general. Las principales formas de tratamiento son la psicoterapia y la farmacoterapia. Este artículo posee como objetivo realizar una breve revisión bibliográfica de los más importantes modelos y técnicas cognitivas y comportamentales de la fobia social. Los modelos antiguos - modelo de déficit de habilidades sociales, modelo de creencias irracionales, modelo de la vulnerabilidad cognitiva y los modelos integrativos - serán revisados brevemente. Serán presentadas las técnicas de tratamiento - como la exposición, reestructuración cognitiva, técnicas de relajamiento y entrenamiento de habilidades sociales. Estudios de metaanálisis sobre la eficacia de la terapia cognitivo-comportamental en el tratamiento de la fobia social también serán descritos.Social phobia is a widespread mental disorder in the general population. The main forms of treatment are psychotherapy and pharmacotherapy. This article has as objective to carry out a brief bibliographical review of the most important cognitive and behavior models and techniques of social phobia. The old models - social

  5. Síndrome de lipodistrofia en pacientes con VIH/sida que reciben terapia antirretroviral de gran actividad en Tepic, México

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    Rogelio Alberto Fernández Argüelles

    2012-06-01

    Full Text Available Objetivo: determinar la relación entre la manifestación del síndrome de lipodistrofia y la terapia antirretroviral de gran actividad en pacientes con VIH/sida. Métodos: se realizó un estudio descriptivo, transversal y correlacional en pacientes con VIH/sida que reciben terapia antirretroviral de gran actividad, atendidos entre marzo y diciembre de 2007 en el Centro Ambulatorio de Prevención y Atención del Sida e Infecciones de Transmisión Sexual (CAPASITS de Tepic, Nayarit, México. La definición y diagnóstico del síndrome se realizó mediante el método de The National Centre in HIV Epidemiology and Clinical Research (NCHECR de Australia. Se utilizó la prueba de chi cuadrado para evaluar la dependencia entre el síndrome y la terapia. Se evaluaron 175 pacientes (128 hombres y 47 mujeres, de 19 a 72 años de edad. Resultados: se diagnosticaron 141 pacientes (80,6 con síndrome de lipodistrofia (IC95% 74,7-86.4 %; el 82,6 % correspondió a hombres y el 74,5 % a mujeres. Según la severidad, el porcentaje fue de 17 % de grado 1, 3 % de grado 2, 10 % de grado 3 y 51 % de grado IV. Las pruebas de chi cuadrado para evaluar dependencia entre el síndrome y la terapia resultaron no significativas. Conclusiones: el síndrome de lipodistrofia severo resulta un serio problema para la apariencia de pacientes de VIH/sida que reciben o no la terapia antirretroviral y que agrega un riesgo cardiovascular importante que debe ser considerado para intentar su prevención o tratamiento. Aunque los resultados pueden presentar sesgos y limitaciones, aportan una aproximación importante para sustentar y planificar intervenciones sanitarias que disminuirían el impacto del síndrome en la salud de los pacientes con VIH/sida.

  6. Perfil Clínico-Epidemiológico de Pacientes que inician Terapia Intensiva con Estatinas para La Prevención Secundaria de Enfermedad Vascular en España

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    Diego Macías Saint Gerons

    2015-01-01

    Full Text Available Las nuevas recomendaciones respecto a la utilización de estatinas potentes y/o dosis altas (terapia intensiva para el tratamiento de la enfermedad cardiovascular se han basado en la extrapolación de los resultados de los ensayos clínicos. El objetivo fue describir el perfil clínico-epidemiológico de los pacientes que inician tratamiento con estatinas para la prevención secundaria en España y estudiar qué factores determinan la utilización de estatinas en terapia intensiva. Métodos: Estudio transversal a partir de 88.751 pacientes ≥45 años con enfermedad cardiovascular que iniciaron tratamiento con estatinas (enero 2007-diciembre 2011. Los tratamientos con dosis superiores a 40 mg/día de simvastatina (o estatina equipotente se consideraron terapia intensiva. Se construyeron modelos de regresión logística multivariante con el fin de examinar la asociación de las variables relacionadas con la prescripción de terapia intensiva respecto al uso de la terapia moderada. Resultados: Se identificó a 16.857 personas adultas que iniciaron tratamiento con estatinas para la prevención secundaria. Los factores predictores para el inicio de terapia intensiva fueron el año de prescripción, sexo masculino (odds ratio ajustada: 1,70; IC95%: 1,44-2,00, edad >75 años (1,39; 1,15-1,69, historia previa de enfermedad isquémica coronaria (1,71; 1,44-2,04, accidente isquémico transitorio de cualquier localización (1,24; 0,97-1,59, tabaquismo (1,62; 1,34-1,95, hipertensión (1,41; 1,20-1,65 y tratamiento reciente con fibratos (2,32; 1,27-4,26. Conclusiones: La utilización de terapia intensiva con estatinas está determinada por el tipo de evento vascular previo y con la edad (>75 años, en los que el balance beneficio-riesgo podría ser discutible. No se encuentran diferencias estadísticamente significativas en función del c-LDL.

  7. Aspectos histopatológicos y receptores hormonales en mucosa endometrial de mujeres posmenopáusicas con terapia hormonal

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    Victoria Valer

    2005-03-01

    Full Text Available Objetivos: Determinar si existe correlación directa entre la terapéutica hormonal administrada en mujeres posmenopaúsicas, el comportamiento de los receptores estrogénicos y progesterónicos en la mucosa endometrial y los cuadros histológicos más frecuentes de patologías endometriales secundarias al tratamiento. Material y Métodos: Estudio prospectivo, longitudinal de 40 mujeres posmenopaúsicas entre los 43 y 60 años con síndrome climatérico, administrándoles 2 mg de 17 beta estradiol y 1 mg de acetato de noretisterona por día, en forma continua y por vía oral. Se realizó una biopsia endometrial basal y otra postratamiento al octavo mes. Las muestras fueron procesadas para estudio histopatológico e inmunohistoquímico para receptores estrogénicos y progesterónicos. Resultados: La menarquia se presentó a una edad promedio de 13,2 años y la menopausia fisiológica entre 42 y 52 años, con una edad promedio de 46,4 años. La sintomatología climatérica mejoró notablemente con la terapia hormonal. El estudio histopatológico de mucosa endometrial basal mostró 10% de hiperplasia simple y 90% de atrofia; postratamiento, todos los casos presentaron atrofia. Los receptores se expresaron en la mucosa endometrial basal: RE (+ 12,5%, RP (++ 12,5%; postratamiento RE (+ 2,5%, RE (+++ 2,5% y RP(+ 2,5%. Conclusiones: La terapia en dosis diarias vía oral de 2 mg de 17 beta estradiol y 1 mg de acetato de noretisterona disminuye la sintomatología climatérica y produce atrofia endometrial en las pacientes con hiperplasia previa. Existe disminución estadísticamente significativa entre la primera muestra basal y la del octavo mes de la expresión de receptores estrogénicos y progesterona.

  8. Modelo de intervención de Terapia Ocupacional Comunitaria con Personas Mayores en el Municipio de Alcobendas

    OpenAIRE

    Viedma, María José de

    2014-01-01

    V Jornadas de actualización en terapia ocupacional: Formación continuada en terapia ocupacional, celebradas en la Universidad Rey Juan Carlos campus de Alcorcón en 2014. Fisioterapia, Terapia Ocupacional, Rehabilitación y Medicina Física

  9. Éxito de la terapia breve centrada en soluciones en su seguimiento

    OpenAIRE

    Domínguez del Val, Ángela

    2011-01-01

    A patir de una muestra (N=40) extraída del Centro Clínico en Terapia Sistémica de la Universidad Pontificia de Salamanca se estudia el éxito que tiene la aplicación de la Terapia Breve Centrada en Soluciones (TBCS)

  10. Asistencia ventricular izquierda para terapia de destino: primera experiencia en septuagenarios

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    Sebastian V. Rojas

    2016-10-01

    Conclusiones: Nuestros resultados iniciales demuestran que el implante mínimamente invasivo de DAVI puede ser realizado de manera eficaz, segura y con buenos resultados en pacientes septuagenarios terapia de destino. Los bajos niveles de eventos adversos durante el seguimiento por 2 años demuestran que la terapia de destino puede ser realizada incluso en pacientes septuagenarios.

  11. Estudo da competência vetorial de Lutzomyia intermedia (Lutz & Neiva, 1912 para Leishmania (Viannia braziliensis, Vianna, 1911 Study of the vectorial competence of Lutzomyia intermedia (Lutz & Neiva, 1912 to Leishmania (Viannia braziliensis, Vianna, 1911

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    Antonio Carlos da Silva

    2001-04-01

    Full Text Available Estudou-se a competência vetorial de Lutzomyia intermedia (Diptera: Psychodidae do Vale do Ribeira (SP para estirpes de Leishmania (Viannia braziliensis (Kinetoplastida: Trypanosomatidae, mediante pesquisa de infectividade natural; exposições de fêmeas silvestres e colonizadas (F1 às lesões de hamsters experimentalmente infectados e testes de transmissão via picada. A infectividade natural e os testes de transmissão revelaram-se negativos e, nas exposições, foram obtidas positividades de 74% (123+/166 dissecados e 70% (115+/164 dissecados para fêmeas silvestres e colonizadas respectivamente, e o desenvolvimento das formas evolutivas compatíveis com o modelo Peripilaria. A suscetibilidade às estirpes testadas associada aos indicadores epidemiológicos concorrem para a suspeita do papel vetorial de Lutzomyia intermedia na região estudada.This paper investigated the vectorial competence of Lutzomyia intermedia (Diptera: Psychodidae in Vale do Ribeira (SP to strains of Leishmania (Viannia braziliensis (Kinetoplastida: Trypanosomatidae, by means of a search for natural infection; exposure of wild and colonized females (F1 to the lesions of experimentally infected hamsters and transmission tests by bite. The natural infection and the transmission tests were negative. In the exposures of Lu. intermedia to infected lesions we found rates of 74% (123+/166 dissected and 70% (115+/164 dissected for the wild and colonized females respectively. The development of the parasites was compatible with the development model of Peripilaria. The susceptibility of the tested strains associated with the epidemiological indicators contribute to the vectorial role suspicion of Lutzomyia intermedia in the studied region.

  12. Does estradiol have an impact on the dipeptidyl peptidase IV enzyme activity of the Prevotella intermedia group bacteria?

    Science.gov (United States)

    Fteita, Dareen; Könönen, Eija; Gürsoy, Mervi; Söderling, Eva; Gürsoy, Ulvi Kahraman

    2015-12-01

    Initiation and development of pregnancy-associated gingivitis is seemingly related to the microbial shift towards specific gram-negative anaerobes in subgingival biofilms. It is known that Prevotella intermedia sensu lato is able to use estradiol as an alternative source of growth instead of vitamin K. The aim of the present study was to investigate the impact of estradiol on the bacterial dipeptidyl peptidase IV (DPPIV) enzyme activity in vitro as a virulent factor of the Prevotella intermedia group bacteria, namely P. intermedia, Prevotella nigrescens, Prevotella pallens, and Prevotella aurantiaca. In all experiments, 2 strains of each Prevotella species were used. Bacteria were incubated with the concentrations of 0, 30, 90, and 120 nmol/L of estradiol and were allowed to build biofilms at an air-solid interface. DPPIV activities of biofilms were measured kinetically during 20 min using a fluorometric assay. The enzyme activity was later related to the amount of protein produced by the same biofilm, reflecting the biofilm mass. Estradiol significantly increased DPPIV activities of the 8 Prevotella strains in a strain- and dose-dependent manner. In conclusion, our in vitro experiments indicate that estradiol regulates the DPPIV enzyme activity of P. intermedia, P. nigrescens, P. pallens, and P. aurantiaca strains differently. Our results may, at least partly, explain the role of estradiol to elicit a virulent state which contributes to the pathogenesis of pregnancy-related gingivitis. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. In vitro effects of N-acetyl cysteine alone and in combination with antibiotics on Prevotella intermedia.

    Science.gov (United States)

    Moon, Ji-Hoi; Jang, Eun-Young; Shim, Kyu Sang; Lee, Jin-Yong

    2015-05-01

    N-acetyl cysteine (NAC) is an antioxidant that possesses anti-inflammatory activities in tissues. In the field of dentistry, NAC was demonstrated to prevent the expression of LPS-induced inflammatory mediators in phagocytic cells and gingival fibroblasts during the inflammatory process, but the effect of NAC on oral pathogens has been rarely studied. Here, we examined the effect of NAC against planktonic and biofilm cells of Prevotella intermedia, a major oral pathogen. NAC showed antibacterial activity against the planktonic P. intermedia with MIC value of 3 mg/ml and significantly decreased biofilm formation by the bacterium even at sub MIC. NAC did not affect the antibiotic susceptibility of planktonic P. intermedia, showing indifference (fractional inhibitory concentration index of 0.5-4) results against the bacterium in combination with ampicillin, ciprofloxacin, tetracycline or metronidazole. On the other hand, viability of the pre-established bacterial biofilm exposed to the antibiotics except metronidazole was increased in the presence of NAC. Collectively, NAC may be used for prevention of the biofilm formation by P. intermedia rather than eradication of the pre-established bacterial biofilm. Further studies are required to explore antibacterial and anti-biofilm activity of NAC against mixed population of oral bacteria and its modulatory effect on antibiotics used for oral infectious diseases.

  14. The relationship of Prevotella intermedia, Prevotella nigrescens and Prevotella melaninogenica in the supragingival plaque of children, caries and oral malodor.

    Science.gov (United States)

    Tanaka, Shoji; Yoshida, Mikako; Murakami, Yukio; Ogiwara, Takako; Shoji, Masao; Kobayashi, Satoko; Watanabe, Sigeru; Machino, Mamoru; Fujisawa, Seiichiro

    2008-01-01

    A relationship between the distribution of periodontal bacteria species and malodor in children has not been sufficiently investigated. The present study was undertaken to determine the presence of 3 periodontopathic bacteria (Prevotella spp. P. intermedia, P. nigrescens, P. melaninogenica) in the supragingival plaques of 3 to 16-year-old children with different oral health conditions and oral malodor. The number of decayed and filled primary teeth (df) and Decayed, Missing and Filled permanent teeth (DMF), Papillary Marginal and Attached gingivitis (PMA) index, Oral Hygiene Index (OHI), and oral malodor of each subject were determined prior to the collection of supragingival plaques. Three periodontopathic bacteria (P. intermedia, P. nigrescens, P. melaninogenica) in supragingival plaques were detected by using an immunoslot blot assay with monoclonal antibodies specific for each microorganism. The frequencies of periodontopathic bacteria in children with and without caries were not significantly different from each other. Positivity for P. intermedia, but not for P. nigrescens or P. melaninogenica was correlated with oral malodor. Oral malodor was also correlated with the debris index, a component of OHI. The group with the higher OHI showed a higher prevalence of periodontopathic bacteria. For the 3 periodontopathic bacteria in the subjects tested, plaques positive for any of them were not age related. However the frequencies of all 3 periodontopathic bacteria were the highest in the 3-6-year olds. The supragingival plaques in children can harbor 3 species of periodontopathic bacteria, P. intermedia, P. nigrescens, and P. melaninogenica.

  15. Differentially regulated proteins in Prevotella intermedia after oxidative stress analyzed by 2D electrophoresis and mass spectrometry.

    Science.gov (United States)

    Santos, Simone G; Diniz, Cláudio G; Silva, Vânia L; Lima, Francisca L; Andrade, Hélida M; Chapeaurouge, Donat A; Perales, Jonas; Serufo, José Carlos; Carvalho, Maria Auxiliadora R; Farias, Luiz M

    2012-02-01

    Prevotella intermedia is a rod-shaped, Gram-negative anaerobic bacterium found in human indigenous microbiota that plays an important role in opportunistic infections. The successful colonization depends on the ability of anaerobes to respond to oxidative stress (OS) in oxygenated tissues as well as to resist oxidative events from the host immune system until anaerobic conditions are present at the infection site. As knowledge of the mechanisms of protection against OS in Prevotella is limited, studies are needed to clarify aspects of molecular biology, physiology and ecology of this bacterium. The aim of this study was to access the proteins differentially regulated in P. intermedia after exposure to molecular oxygen by using two-dimensional gel electrophoresis (2DE) associated with the approach of MALDI-TOF/TOF Tandem Mass Spectrometry. The identity of the protein was evaluated by database search for homologous genomic sequences of P. intermedia strain 17 (TIGR). Twenty five out of 72 proteins found were identified as up-regulated (17) or down-regulated (9). These proteins were related to a variety of metabolic process, some of which could be associated to antioxidant and redox regulatory roles. Our data indicate that OS may stimulate an adaptive response in P. intermedia whose effect on its biology may be evidenced by the increase in aerotolerance and changes in protein abundance in the oxygen adapted cells. Copyright © 2011 Elsevier Ltd. All rights reserved.

  16. Ciência Ocupacional e Terapia Ocupacional: algumas reflexões/ Occupational Science and Occupational Therapy: some reflections

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    Elson Ferreira Costa

    2017-11-01

    por la historia de vida de cada individuo. Por lo tanto, este artículo dirigido a través de una reflexión teórica entender las ocupaciones, bajo los auspicios de los supuestos de Ciencia de la Ocupación y su relación con la terapia ocupacional. Se trata de un estudio de revisión narrativa como método. Los resultados apuntan que una de las líneas de investigación desarrolladas en la Ciencia Ocupacional es la que enfoca la forma, la función y el significado de la ocupación. La Ciencia Ocupacional trata de cuestiones universales y singulares sobre la ocupación, con posibilidades de aplicación a la Terapia Ocupacional. Así, esta disciplina puede ser una fuente de evidencia para esta profesión.Palabras-clave: Ciencia; Ocupaciones; Terapia ocupacional.

  17. Terapia nutricional no traumatismo cranioencefálico grave

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    Brenno Belazi Nery de Souza Campos

    2012-03-01

    Full Text Available O objetivo do presente artigo é revisar a literatura e organizar os principais achados, gerando recomendações baseadas nas melhores evidências encontradas relativas à terapia nutricional nos casos de traumatismo cranioencefálico. O traumatismo cranioencefálico permanece uma patologia altamente letal, apesar dos avanços em seu diagnóstico e tratamento. Poucas intervenções terapêuticas tem se mostrado eficazes em melhorar este quadro. Há múltiplas alterações metabólicas e hidroeletrolíticas decorrentes do traumatismo cranioencefálico, caracterizadas por um estado hipermetabólico associado a um intense catabolismo, que levam a necessidades nutricionais específicas. Na literatura atual não há diretrizes específicas para terapia nutricional em pacientes vítimas de traumatismo cranioencefálico grave, mas há muitos dados interessantes e questões que estão sendo melhores estudadas, possibilitando um melhor direcionamento da terapia nutricional neste cenário. Além de avaliação e acompanhamento por uma equipe multiprofissional qualificada e treinada para estas questões, a introdução precoce do suporte nutricional, a utilização preferencial da via enteral com a infusão adequada de calorias, o uso de formulações adequadas e nutricionalmente equilibradas para cada caso específico, associadas a utilização de imunonutrientes específicos, melhor controle hidroeletrolítico e metabólico, além de melhor entendimento fisiopatológico e das consequências das próprias terapêuticas instituídas, parece modificar os desfechos destes casos.

  18. Introducción a la terapia asistida con animales: tipologías de terapia en España

    OpenAIRE

    García Martín, Carmen

    2011-01-01

    Se hace una revisión teórico-bibliográfica sobre la terapia asistida con animales, centrándose en animales como perros, caballos y delfines, en los diferentes ámbitos de intervención en España, incluyendo datos recogidos sobre los resultados de este tipo de terapia en algunas asociaciones del país

  19. Intraspecies Variability Affects Heterotypic Biofilms of Porphyromonas gingivalis and Prevotella intermedia: Evidences of Strain-Dependence Biofilm Modulation by Physical Contact and by Released Soluble Factors.

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    Graziela Murta Barbosa

    Full Text Available It is well known that strain and virulence diversity exist within the population structure of Porphyromonas gingivalis. In the present study we investigate intra- and inter-species variability in biofilm formation of Porphyromonas gingivalis and partners Prevotella intermedia and Prevotella nigrescens. All strains tested showed similar hydrophobicity, except for P. gingivalis W83 which has roughly half of the hydrophobicity of P. gingivalis ATCC33277. An intraspecies variability in coaggregation of P. gingivalis with P. intermedia was also found. The association P. gingivalis W83/P. intermedia 17 produced the thickest biofilm and strain 17 was prevalent. In a two-compartment system P. gingivalis W83 stimulates an increase in biomass of strain 17 and the latter did not stimulate the growth of P. gingivalis W83. In addition, P. gingivalis W83 also stimulates the growth of P. intermedia ATCC25611 although strain W83 was prevalent in the association with P. intermedia ATCC25611. P. gingivalis ATCC33277 was prevalent in both associations with P. intermedia and both strains of P. intermedia stimulate the growth of P. gingivalis ATCC33277. FISH images also showed variability in biofilm structure. Thus, the outcome of the association P. gingivalis/P. intermedia seems to be strain-dependent, and both soluble factors and physical contact are relevant. The association P. gingivalis-P. nigrescens ATCC33563 produced larger biomass than each monotypic biofilm, and P. gingivalis was favored in consortia, while no differences were found in the two-compartment system. Therefore, in consortia P. gingivalis-P. nigrescens physical contact seems to favor P. gingivalis growth. The intraspecies variability found in our study suggests strain-dependence in ability of microorganisms to recognize molecules in other bacteria which may further elucidate the dysbiosis event during periodontitis development giving additional explanation for periodontal bacteria, such as P

  20. Intraspecies Variability Affects Heterotypic Biofilms of Porphyromonas gingivalis and Prevotella intermedia: Evidences of Strain-Dependence Biofilm Modulation by Physical Contact and by Released Soluble Factors.

    Science.gov (United States)

    Barbosa, Graziela Murta; Colombo, Andrea Vieira; Rodrigues, Paulo Henrique; Simionato, Maria Regina Lorenzetti

    2015-01-01

    It is well known that strain and virulence diversity exist within the population structure of Porphyromonas gingivalis. In the present study we investigate intra- and inter-species variability in biofilm formation of Porphyromonas gingivalis and partners Prevotella intermedia and Prevotella nigrescens. All strains tested showed similar hydrophobicity, except for P. gingivalis W83 which has roughly half of the hydrophobicity of P. gingivalis ATCC33277. An intraspecies variability in coaggregation of P. gingivalis with P. intermedia was also found. The association P. gingivalis W83/P. intermedia 17 produced the thickest biofilm and strain 17 was prevalent. In a two-compartment system P. gingivalis W83 stimulates an increase in biomass of strain 17 and the latter did not stimulate the growth of P. gingivalis W83. In addition, P. gingivalis W83 also stimulates the growth of P. intermedia ATCC25611 although strain W83 was prevalent in the association with P. intermedia ATCC25611. P. gingivalis ATCC33277 was prevalent in both associations with P. intermedia and both strains of P. intermedia stimulate the growth of P. gingivalis ATCC33277. FISH images also showed variability in biofilm structure. Thus, the outcome of the association P. gingivalis/P. intermedia seems to be strain-dependent, and both soluble factors and physical contact are relevant. The association P. gingivalis-P. nigrescens ATCC33563 produced larger biomass than each monotypic biofilm, and P. gingivalis was favored in consortia, while no differences were found in the two-compartment system. Therefore, in consortia P. gingivalis-P. nigrescens physical contact seems to favor P. gingivalis growth. The intraspecies variability found in our study suggests strain-dependence in ability of microorganisms to recognize molecules in other bacteria which may further elucidate the dysbiosis event during periodontitis development giving additional explanation for periodontal bacteria, such as P. gingivalis and P

  1. Desarrollo curricular, conciencia ambiental y tecnologia para estudiantes de intermedia: Una investigacion en accion

    Science.gov (United States)

    Rodriguez Ramos, Teresita

    Se llevó a cabo una investigación en acción con los propósitos de 1) documentar las relaciones de las tecnologías de la información y la comunicación en las clases de ciencias de escuela intermedia como elemento de apoyo cuando se aborda el tema ambiental y sus conceptos pertinentes, a partir de las observaciones de la investigadora, así como las entrevistas y diarios reflexivos de los estudiantes de una escuela intermedia en la zona metropolitana, y luego 2) diseñar una unidad instruccional sobre el tema ambiental que integre actividades tecnologías para el curso de ciencias de la escuela intermedia según el modelo PROCIC y las observaciones que hayan iniciado los estudiantes participantes. Finalmente, se plantearon las implicaciones educativas para el currículo del Programa de Ciencias al instrumentar este modelo de unidad mediante PROCIC, e integrado la tecnología y el tema ambiental. Los hallazgos se analizaron y se categorizaron de acuerdo con las preguntas de investigación. El hallazgo principal de la investigación aborda las cuatro relaciones centrales en las que se articula la utilización de las tecnologías y sus aplicaciones en la clase de ciencias. Estas cuatro relaciones que recogen la posición de los estudiantes son: 1) Perspectiva de los estudiantes hacia la tecnología. 2) Participación de los estudiantes en los aspectos docentes. 3) Aprendizaje estudiantil sobre el ambiente, y 4) Conciencia ambiental en relación con la vida diaria. Estas relaciones ponen de manifiesto,cómo se plantea en las implicaciones, la necesidad de más investigación en acción en la sala de clases, la importancia—como tema transversal—de la conciencia ambiental mediante la tecnología al construir conocimientos significativos dentro y fuera de la escuela, asó como, valorar la investigación y la dialogicidad en la sala de clases como actividades que obligan al reexamen de la práctica didáctica en su formas curriculares de objetivos, recursos

  2. Different water use strategies of juvenile and adult Caragana intermedia plantations in the Gonghe Basin, Tibet Plateau.

    Directory of Open Access Journals (Sweden)

    Zhiqing Jia

    Full Text Available In a semi-arid ecosystem, water is one of the most important factors that affect vegetation dynamics, such as shrub plantation. A water use strategy, including the main water source that a plant species utilizes and water use efficiency (WUE, plays an important role in plant survival and growth. The water use strategy of a shrub is one of the key factors in the evaluation of stability and sustainability of a plantation.Caragana intermedia is a dominant shrub of sand-binding plantations on sand dunes in the Gonghe Basin in northeastern Tibet Plateau. Understanding the water use strategy of a shrub plantation can be used to evaluate its sustainability and long-term stability. We hypothesized that C. intermedia uses mainly deep soil water and its WUE increases with plantation age. Stable isotopes of hydrogen and oxygen were used to determine the main water source and leaf carbon isotope discrimination was used to estimate long-term WUE. The root system was investigated to determine the depth of the main distribution. The results showed that a 5-year-old C. intermedia plantation used soil water mainly at a depth of 0-30 cm, which was coincident with the distribution of its fine roots. However, 9- or 25-year-old C. intermedia plantations used mainly 0-50 cm soil depth water and the fine root system was distributed primarily at soil depths of 0-50 cm and 0-60 cm, respectively. These sources of soil water are recharged directly by rainfall. Moreover, the long-term WUE of adult plantations was greater than that of juvenile plantations.The C. intermedia plantation can change its water use strategy over time as an adaptation to a semi-arid environment, including increasing the depth of soil water used for root growth, and increasing long-term WUE.

  3. Different water use strategies of juvenile and adult Caragana intermedia plantations in the Gonghe Basin, Tibet Plateau.

    Science.gov (United States)

    Jia, Zhiqing; Zhu, Yajuan; Liu, Liying

    2012-01-01

    In a semi-arid ecosystem, water is one of the most important factors that affect vegetation dynamics, such as shrub plantation. A water use strategy, including the main water source that a plant species utilizes and water use efficiency (WUE), plays an important role in plant survival and growth. The water use strategy of a shrub is one of the key factors in the evaluation of stability and sustainability of a plantation. Caragana intermedia is a dominant shrub of sand-binding plantations on sand dunes in the Gonghe Basin in northeastern Tibet Plateau. Understanding the water use strategy of a shrub plantation can be used to evaluate its sustainability and long-term stability. We hypothesized that C. intermedia uses mainly deep soil water and its WUE increases with plantation age. Stable isotopes of hydrogen and oxygen were used to determine the main water source and leaf carbon isotope discrimination was used to estimate long-term WUE. The root system was investigated to determine the depth of the main distribution. The results showed that a 5-year-old C. intermedia plantation used soil water mainly at a depth of 0-30 cm, which was coincident with the distribution of its fine roots. However, 9- or 25-year-old C. intermedia plantations used mainly 0-50 cm soil depth water and the fine root system was distributed primarily at soil depths of 0-50 cm and 0-60 cm, respectively. These sources of soil water are recharged directly by rainfall. Moreover, the long-term WUE of adult plantations was greater than that of juvenile plantations. The C. intermedia plantation can change its water use strategy over time as an adaptation to a semi-arid environment, including increasing the depth of soil water used for root growth, and increasing long-term WUE.

  4. Rendimiento escolar: su abordaje desde la terapia de juego familiar

    OpenAIRE

    Padilla, Xóchitl; Ruiz, Verónica

    2016-01-01

    La evaluación del área emocional cuando existe bajo aprovechamiento escolar permite tener más herramientas para el diagnóstico. Adicionalmente la revisión bibliográfica, el trabajo interdisciplinario y el juego ofrecen herramientas para la toma de decisiones. En este trabajo, se describe el proceso de evaluación e intervención clínica de una menor de nueve años con dificultades académicas. La madre solicitó terapias de aprendizaje debido a “bajas calificaciones y problemas de socialización”. ...

  5. Estresse ocupacional na enfermagem atuante na unidade de terapia intensiva

    OpenAIRE

    Borges Teixeira, Larissa; Portela Veloso, Lorena Uchôa; Universidade Estadual do Piauí; Pereira Ribeiro, Ítalo Arão; Universidade Estadual do Piauí; Nunes de Oliveira, Tauane; Instituto de Ensino Superior Franciscano; Leal Cortez, Antônio Carlos; Universidade Estadual do Piauí

    2017-01-01

    Introdução: O estresse tem sido um dos problemas que mais tem acometido a equipe de enfermagem dentro da unidade de terapia intensiva (UTI), causando prejuízo funcional, bem como instabilidade na saúde desses profissionais. Objetivo: Descrever, através da revisão integrativa, o estresse no trabalho da enfermagem na UTI, bem como identificar fatores, sinais e sintomas associados ao estresse ocupacional. Método: Trata-se de uma pesquisa integrativa, utilizando como banco de dados a Biblioteca V...

  6. INVESTIGACION Y DESARROLLO DE TERAPIAS BIOLOGICAS OPTIMIZADAS PARA TUMORES UROLOGICOS

    OpenAIRE

    SALAZAR ONFRAY, FLAVIO

    2011-01-01

    El proyecto FONDEF INVESTIGACIÓN Y DESARROLLO DE TERAPIAS BIOLÓGICAS OPTIMIZADAS PARA TUMORES UROLÓGICOS buscaba elaborar nuevas alternativas terapéuticas oncológicas, enfocadas en cáncer de próstata hormono-resistente, basados en el uso de la tecnología RapidDC, nombre modificado finalmente a TAPCells, junto con la capacidad inmunopotenciadora del nuevo adyuvante Inmunocyanin. El desarrollo de este proyecto avanzó desde la habilitación de unidades físicas (laboratorios) para la inve...

  7. El enfoque sistémico en terapia familiar

    OpenAIRE

    Zevallos Vega, Roxana

    2013-01-01

    The author reviews sorne aspects of the onset of Family Therapy. The different stages are described, emphasizing the work of authors such as Hoffrnan, Minuchin, Maturana, among others. The beginning of Family Therapy in Peru is outlined and professional groups working in the field are mnentioned La autora resalta algunos aspéctos de la aparición de la Terapia Familiar y describe las etapas por las que ha evolucionado, destacando el rol de autores como Hoffman, Minuchin, Maturana, entre otr...

  8. Terapia gênica para osteoporose Gene therapy for osteoporosis

    Directory of Open Access Journals (Sweden)

    Rafael Pacheco da Costa

    2011-01-01

    Full Text Available A osteoporose é considerada um dos problemas de saúde mais comuns e sérios da população idosa mundial. É uma doença crônica e progressiva, caracterizada pela diminuição da massa óssea e deterioração da microarquitetura do tecido ósseo. A terapia gênica representa uma nova abordagem para o tratamento da osteoporose e tem como princípio devolver a função comprometida pelo metabolismo. Esta revisão visa focar os trabalhos relevantes desenvolvidos nos últimos anos, disponibilizados nas bases de dados médicas, e que utilizaram a terapia gênica para o tratamento da osteoporose em modelos animais, bem como, as perspectivas futuras desta terapia. A maioria dos estudos utiliza os genes BMPs, PTH e OPG na tentativa de restabelecer a massa óssea. Apesar da carência de novas moléculas, todos os genes empregados nos estudos se mostraram eficientes no tratamento da doença. Os benefícios que a terapia gênica proporcionará aos pacientes no futuro devem contribuir substancialmente para o aumento na qualidade de vida dos idosos. Em breve, protocolos clínicos envolvendo humanos irão beneficiar os indivíduos com osteoporose.Osteoporosis is considered one of the most common and serious problems affecting the elderly population worldwide. It is a chronic and progressive disease, characterized by decreased bone mass and degeneration of the microarchitecture of the bone tissue. Gene therapy represents a new approach in osteoporosis treatment, and its main function is to restore the compromised function in the metabolism. This review aims to elucidate the main studies on gene therapy in recent years, in the medical databases, that use gene therapy for the treatment of osteoporosis in animal models, as well as the future prospects of this therapy. The majority of the studies use the BMP, PTH and OPG genes, in an attempt to reestablish bone mass. Despite the lack of new molecules, all genes employed in these studies have proven to be

  9. ESTUDIOS SEROEPIDEMIOLÓGICOS

    OpenAIRE

    Neus Cardeñosa Marín

    2009-01-01

    Los estudios seroepidemiológicos permiten estudiar la distribución de las enfermedades de manera indirecta, mediante la detección sérica de marcadores de infección y de inmunidad. Estos estudios son aplicables en investigaciones en salud pública, investigaciones destinadas a determinar la prevalencia o incidencia de ciertas infecciones en particular, para evaluar programas de control e inmunización y para evaluar antecedentes históricos cuando un nuevo microorganismo es descubierto. En ...

  10. Comportamiento de la terapia SU-JOK en pacientes ansiosos en un área de salud

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    Carmen Luisa González Hidalgo

    2012-01-01

    Full Text Available Abstract This article presents the results of a 2009 research paper. This papers purpose was to determine the effect of Su-Jok therapy in anxious patients. Anxiety is one of the most frequent causes in psychology sessions. The research work allowed for evaluation of Su-Jok therapy performance in anxious patients at “Céspedes Argote” health center in Arroyo Naranjo, Havana, 2009. An empiric, observational, descriptive and transverse study was carried out. Patients were chosen from a simple random sampling of 144 patients selected from a group of 325 anxious patients. The collected data was processed in Microsoft Excel database; by statistical package SPSS, Version 11.0. Cattell questionnaire was used to assess their anxiety and Su-Jok therapy was applied as the sole treatment. Better evolved patients (inferior and average anxiety who reached 74% were able to relieve the anxiety during the first weeks of treatment obtaining better results than those who attended sessions every day. It was concluded that Su-Jok therapy is an effective way to reduce patient’s anxiety. Resumen El presente artículo presenta los resultados de un trabajo de investigación del año 2009, que permitió determinar el efecto de la terapia Su-Jok en pacientes ansiosos del Policlínico “Céspedes Argote” de Arroyo Naranjo, Habana. Se realizó un estudio empírico, observacional, descriptivo y transversal. Del universo de 325 pacientes ansiosos, se seleccionó una muestra probabilística aleatoria simple de 144. Se utilizó el cuestionario Cattell para medir la ansiedad y se aplicó como único tratamiento la terapia Su-Jok. Los datos recopilados se procesaron en una base de datos en Microsoft Excel, mediante el paquete estadístico SPSS, versión 11.0. El 74% de la muestra logró aliviar la ansiedad desde las primeras semanas y hubo mejores resultados en los que asistieron siempre. En conclusión, se demostró la efectividad del tratamiento con terapia Su-Jok en

  11. Current status of Complementary Therapies in Spain in nursing degree Situación actual de las Terapias Complementarias en España en el Grado de Enfermería Estado atual do ensino de Terapias Complementares na formação superior de Enfermagem na Espanha

    Directory of Open Access Journals (Sweden)

    Ana Belén Fernández-Cervilla

    2013-06-01

    Full Text Available OBJECTIVE: Describe the current situation of complementary therapies in nursing education in schools and nursing schools in Spain. METHOD: Descriptive study. Study population all faculties andschools ascribed to Spain. Data collection through observation sheet. Analysis units Curricula. Variables (credits, course type, location, type of therapy. Descriptive analysis of relative and absolute data using Excel spreadsheet. RESULTS: Most of the faculties and schoolsofnursingscrutinized the subject of Complementary Therapies has disappeared and those that aparace is an optional subject. CONCLUSION: Complementary Therapies training in Spain is deficient because it is not collected as a core subject and / or obligatory. The absence of Complementary Therapies in the curriculum, questions such as their value in training, conceptualization which teachers, the impact on quality of care, training of teachers responsible for teaching the subject, as well as the number of credits and the course being in the degree of Grado. OBJETIVO: Describir la situación actual de las Terapias Complementarias en la formación enfermera en las escuelas y facultades de enfermería en España. MÉTODO: estudio observacional descriptivo transversal. Población de estudio todas las facultades, escuelas públicas y adscritas de España. Recolección de datos mediante una ficha de observación. Unidades de análisis los Planes de Estudio. Variables (créditos, tipo de asignatura, ubicación, tipo de terapia. Análisis descriptivo de los datos relativos y absolutos mediante hoja de Excel. RESULTADOS: en la mayoría de las facultades y escuelas escrutadas la asignatura de Terapias Complementarias ha desaparecido y en aquellas que aparece es una asignatura optativa. CONCLUSÍON: la formación en Terapias Complementarias en España es deficiente, debido a que no se recoge como asignatura troncal y/o obligatoria. La ausencia de las Terapias Complementarias en el currículum, plantea

  12. La periferia espontánea en las ciudades intermedias latinoamericanas: perspectivas de solución desde la dimensión territorial-ambiental de la sostenibilidad

    Directory of Open Access Journals (Sweden)

    José Jorge Peralta Arias

    2017-05-01

    Full Text Available La investigación aborda el problema de la insostenibilidad de la periferia espontánea en las ciudades intermedias latinoamericanas, cuya informalidad ha determinado un crecimiento discontinuo, una baja densidad y el déficit de infraestructuras y dotaciones; a esto, se suman los riesgos futuros por desastres de origen natural asociados al cambio climático. En dicho contexto, el trabajo se plantea como objetivo central contribuir a solucionar este problema, a partir de una propuesta metodológica para el estudio de las periferias espontáneas de las ciudades latinoamericanas de escala intermedia, desde la perspectiva del desarrollo sostenible, poniendo énfasis en la dimensión física, ambiental y climática. Para ello, la investigación se organiza en tres partes: revisión y análisis de la información existente sobre la temática y consulta a expertos, para la determinación de criterios; la determinación de acciones prioritarias; y una propuesta de estructura que sustenta un sistema de indicadores. Los resultados del trabajo proponen una batería de treinta y nueve (39 indicadores referenciales, articulados en cuatro subsistemas relacionados con: la vulnerabilidad física-ambiental y climática, el eje ambiental, el desarrollo urbano y la cohesión social. Estos constituyen la principal conclusión del trabajo, el que, se espera, será útil para comenzar a tomar las primeras decisiones frente al problema de este tipo de periferias.

  13. Terapias combinadas en la esquizofrenia, ¿Agregamos, mezclamos y confundimos, o bien, deconstruímos, y combinamos?.

    OpenAIRE

    Jorge Luis Tizón García

    2004-01-01

    Se realiza una reflexión sobre el estado actual de los programas terapéuticos o terapias que intentan combinar diversos tipos de terapias biológicas, psicológicas y psicosociales. Se ilustra el tema con viñetas clínicas de tratamientos de pacientes agorafóbicos y esquizofrénicos. La conclusión fundamental es que, hoy por hoy, con algunas excepciones, más que terapias combinadas se están practicando agregaciones de terapias o incluso mezclas más o menos confusas de terapias. Entre otros motivo...

  14. In Vitro Antibacterial and Antifungal Activity of Lavandula x intermedia Emeric ex Loisel. ‘Budrovka’

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    Sanda Vladimir-Knezevic

    2011-05-01

    Full Text Available This study aimed to evaluate the in vitro antibacterial and antifungal activities of Lavandula x intermedia Emeric ex Loisel. ‘Budrovka’, an indigenous Croatian cultivar of lavandin. For that purpose the activity of ethanolic extracts of flowers, inflorescence stalks and leaves against thirty one strains of bacteria, yeasts, dermatophytes and moulds were studied using both the agar well diffusion and broth dilution assays. Among the investigated extracts found to be effective against a broad spectrum of microorganisms, the flower extract was considered to be the most potent one. Linalool and rosmarinic acid, as the most abundant constituents found, are very likely major contributors to the observed antimicrobial effects. The results suggest that flowers of lavandin ‘Budrovka’ could serve as a rich source of natural terpene and polyphenol antimicrobial agents.

  15. Histopathological hoof laminar changes in horses with Pituitary Pars Intermedia Adenoma: cases report

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    L. M. Laskoski

    2015-10-01

    Full Text Available ABSTRACTLaminitis in horses is often associated with endocrine disorders, especially the pituitary pars intermedia dysfunction (PPID in older animals. Morphologic exams of the laminar tissue of the hoof were performed in two horses with suspected PPID, with no clinical signs of laminitis. Changes compatible with laminitis of endocrine origin were observed, such as rounding of the nuclei of the basal cells, thinning and stretching of the secondary epidermal laminae and tissue proliferation. PPID horses with no clinical signs of laminitis may be affected by lesions of the laminar tissue of the hoof that compromise the integrity of the dermal-epidermal junction and may develop clinical symptoms of the disease. It has been suggested that the development stage of endocrine laminitis is longer, but further studies should be conducted to confirm it.

  16. [Phylogenetic diversity of microorganisms associated with the deep-water sponge Baikalospongia intermedia].

    Science.gov (United States)

    Kalyzhnaya, O V; Itskovich, V B

    2014-07-01

    The diversity of bacteria associated with deep-water sponge Baikalospongia intermedia was evaluated by sequence analysis of 16S rRNA genes from two sponge samples collected in Lake Baikal from depths of 550 and 1204 m. A total of 64 operational taxonomic units, belonging to nine bacterial phyla, Proteobacteria (classes Alphaproteobacteria,. Betaproteobacteria, Gammaproteobacteria, and Deltaproteobacteria), Actinobacteria, Planctomycetes, Cloroflexi, Verrucomicrobia, Acidobacteria, Chlorobi, and Nitrospirae, including candidate phylum WS5, were identified. Phylogenetic analysis showed that the examined communities contained phylotypes exhibiting homology to uncultured bacteria from different lake ecosystems, freshwater sediments, soil and geological formations. Moreover, a number of phylotypes were relative to psychrophilic, methane-oxidizing, sulfate-reducing bacteria, and to microorganisms resistant to the influence of heavy metals. It seems likely that the unusual habitation conditions of deep-water sponges contribute to the taxonomic diversity of associated bacteria and have an influence on the presence of functionally important microorganisms in bacterial communities.

  17. Flujo vaginal. Etiología en una unidad intermedia de salud

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    Luz Marina Alzate

    1987-12-01

    Full Text Available En una serie de 584 mujeres que consultaron por flujo vaginal a una Unidad Intermedia de Salud se halló que la entidad más frecuente era la vaginosis bacteriana (vaginitis inespecífica o por Gardnerella vaginalis con un 34,8% seguida por la candidiasis (9,4% y la tricomoniasis (43%; hubo también infecciones mixtas (7,2%; la candidiasis, la tricomoniasis y las infecciones mixtas, pero no la vaginosis bacteriana, fueron más frecuentes en mujeres embarazadas; se hace énfasis en la facilidad de diagnosticar vaginosis bacteriana en el laboratorio corriente con base en criterios de muy fácil determinación.

  18. A chinese case of prevotella intermedia and streptococcus constellatus intracranial mixed infection.

    Science.gov (United States)

    Mo, Shanying; Wei, Liuhua; Chen, Hongmou; Li, Rui; Li, Shuping; Luo, Guolan

    2018-02-01

    Streptococcal Species is increasingly recognized as a potentially preventable emerging infection in human's brain with high prevalence around the world. Streptococcus constellatus is one of the most common pathogens. Meanwhile, anaerobic bacteria are the rare causes for intracranial infection. To date, intracranial mixed infection caused by Prevotella intermedia and Streptococcus constellatus has not been reported. We reported a Chinese case to raise the global awareness of severity of the intracranial mixed infection. Here, we illustrated the epidemiological risk factors, clinical manifestations and outcomes of the patient. For patients who suffer from exacerbated brain infection with fetid cerebrospinal fluid, early repeated imaging is urgently needed and empiric antibiotic therapy should consider anaerobic and aerobic bacteria in these situations.

  19. de estudios observacionales

    Directory of Open Access Journals (Sweden)

    Erik von Elm

    2008-01-01

    un documento de explicación y elaboración al que puede accederse libremente en los sitios web de PLoS Medicine, Annals of Internal Medicine y Epidemiology. Esperamos que la declaración STROBE contribuya a mejorar la calidad de la publicación de los estudios observacionales.

  20. ESTUDIOS SEROEPIDEMIOLÓGICOS

    Directory of Open Access Journals (Sweden)

    Neus Cardeñosa Marín

    2009-01-01

    y la evaluación de los programas de vacunaciones. Para la buena calidad de los estudios seroepidemiológicos es importante que la muestra de sueros utilizada sea representativa de la población total que queramos estudiar, y se debe tener en cuenta que la prueba serológica a utilizar debe tener una elevada sensibilidad.

  1. Resumen estudios sobre tabaquismo

    OpenAIRE

    Gónzalez, M.M. (M.)

    2009-01-01

    Este documento se realizó para publicar en la página Web de la Gerencia de Atención primaria del Área III. Este documento es una recopilación de estudios sobre tabaquismo. Gerencia de Atención Primaria Área III

  2. Side effects of Deferasirox Iron Chelation in Patients with Beta Thalassemia Major or Intermedia

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    Murtadha Al-Khabori

    2013-03-01

    Full Text Available Objectives: Chelating agents remain the mainstay in reducing the iron burden and extending patient survival in homozygous beta-thalassemia but adverse and toxic effects may increase with the institution and long term use of this essential therapy. This study aimed to estimate the incidence of deferasirox (DFX side effects in patients with thalassemia major or intermedia.Methods: A retrospective study of 72 patients (mean age: 20.3±0.9 yrs; 36 male, 36 female with thalassemia major or intermedia treated at Sultan Qaboos University Hospital, Oman, was performed to assess the incidence of side effects related to deferasirox over a mean of 16.7 month follow-up period.Results: Six patients experienced rashes and 6 had gastro-intestinal upset. DFX was discontinued in 18 patients for the following reasons: persistent progressive rise(s in serum creatinine (7 patients; 40% mean serum creatinine rise from baseline, feeling unwell (2, severe diarrhea (1, pregnancy (1, death unrelated to chelator (2 and rise in serum transaminases (2. Three patients were reverted to desferoxamine and deferiprone combination therapy as DFX was no longer biochemically effective after 18 months of therapy. There was no correlation between baseline serum ferritin and serum creatinine or a rise in serum creatinine. Cardiac MRI T2* did not change with DFX therapy. However, there was an improvement in liver MRI T2* (p=0.013.Conclusion: Renal side effects related to deferasirox appear to be higher than those reported in published clinical trials. Further larger studies are required to confirm these findings.

  3. Aplicabilidade de indicador de qualidade subjetivo em Terapia Intensiva Aplicación de indicadores subjectivos de calidad en Terapia Intensiva Application of subjective quality indicators in Intensive Care

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    Sônia Regina de Oliveira e Silva de Souza

    2006-04-01

    Full Text Available Objetivamos descrever a percepção dos clientes em relação à hospitalização na Unidade de Terapia Intensiva (UTI. Desenvolvemos um estudo descritivo com abordagem qualitativa, na UTI de um hospital universitário do Rio de Janeiro, no período de maio/2003 a maio/2004. Participaram do estudo 32 clientes após alta da UTI em condição de narrar sua hospitalização. A coleta dos dados ocorreu por via de um questionário. Consideramos que os clientes demonstraram satisfação a assistência de enfermagem intensivista, o que incomoda são os estressores físicos e ambientais. O estudo aponta questões que necessitam de uma contínua discussão considerando o estresse que permeia as atividades e o ambiente da UTI e destaca a relevância do trabalho com indicadores de qualidade subjetivos em terapia intensiva.Tenemos como objetivo describir la percepión de los clientes en relación a la hospitalización en la Unidad de Cuidados Intensivos. Desarrollamos un estudio descriptivo con enfoque cualitativo en la Unidad de Cuidados Intensivos de un Hospital Universitario de Rio de Janeiro, en el periodo de mayo/2003 a mayo/2004. Participarón del estudio 32 clientes tras la alta de esta Unidad condición de narrar su hospitalización. Los datos fueron recogidos por un cuestionario. Consideramos que los clientes demostrarón satisfacción con respecto a la asistencia de enfermería intensiva y lo que les incomoda son los estreses físicos y ambientales. El estudio apunta cuestiones que necesitan una continua discusión considerando el estrés que rodea las actividades, el ambiente de la Unidade de Cuidados Intensivos y destaca la relevancia del trabajo con ondicativos de calidad subjetivos en Terapia intensiva.Our aim is to describe the clients' perception related to to the admission in the Intensive Care. We have developed a descriptive study based on a qualitative approach in the Intensive Care in a university hospital in RJ, from May, 2003 to May

  4. Reflexões acerca da assistência de enfermagem e o discuso de humanização em terapia intensiva Reflexiones referentes a la ayuda de enfermería y el discurso de la humanización en terapia intensiva Reflections concerning the assistance of nursing and the speech of humanization in intensive therapy

    Directory of Open Access Journals (Sweden)

    Roberto Carlos Lyra da Silva

    2008-03-01

    Full Text Available Este estudo é uma reflexão que objetiva buscar como produção científica de enfermagem o cuidado que envolve o uso de tecnologias/máquinas em unidades de terapia intensiva, a par tir do entendimento acerca do cuidado, tecnologia e humanização, e as implicações para a prática de cuidar em unidades tecnológicas de clientes críticos. Traz reflexões acerca da temática "humanização em terapia intensiva", a par tir do aprofundamento das leituras e discussão dos dados, na construção de uma tese em curso na Escola Anna Nery, cujo objeto de investigação é "o significado do cuidado em unidades de terapia intensiva". Verificou-se que qualquer que seja a reflexão acerca das práticas de cuidar em terapia intensiva que desconsidere seus conceitos, o contexto e suas bases possibilitadoras poderá incorrer em equívocos comprometedores, capazes de se constituírem obstáculos epistemológicos.El estudio es una reflexión la cual objetiva buscar como producción científica de enfermeria el cuidado que implica el uso de tecnologias y de maquinas en unidades de la terapia intensiva, del referente cuidado, la tecnología y a la humanización, y sus implicaciones para el práctico de cuidar en unidades tecnológicas de clientes críticos. Está es una reflexión referente a "humanización en terapia intensiva", a par tir de las lecturas y discusión de los datos, en la construcción de una tesis de doutorado en curso en la Escuela de Enfermeria Ana Nery, cujo objeto de la investigación es el significado del cuidado en unidades del terapia intensiva. Fue verificado cuales sean las reflexiones referente a las prácticas de cuidar en la terapia intensiva que no considera sus conceptos, el contexto y sus bases, posiblemente, podrá incurrir en errores para comprometerse, capaz de constituir obstáculos epistemologicos.This study is a reflection that objective to search as scientific production of nursing the care that involves the use of

  5. Studies on the feeding habits of Lutzomyia (N. intermedia (Diptera, Psychodidae, vector of cutaneous leishmaniasis in Brazil Estudos sobre os hábitos alimentares de Lutzomyia (N. intermedia (Diptera, Psychodidae, vetor de leishmaniose cutânea no Brasil

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    Margarete Martins dos Santos Afonso

    2005-12-01

    Full Text Available The precipitin test was applied to identify the blood meal sources of Lutzomyia intermedia, collected in two distinct habitats: inside houses and in the peridomicile, in Mesquita, Rio de Janeiro State, Brazil, a transmission area of Leishmania (V. braziliensis. The following antisera were tested: human, avian, equine, rodent, and opossum. From a total of 370 females analyzed, 128 specimens from the domicile and 59 from the peridomicile reacted with specific antisera. The anthropophily of L. intermedia was confirmed in both habitats; likewise, the feeding of this sand fly species on domestic animals, observed in previous entomological surveys, was confirmed by the strong reactivity with avian, canine, and equine antisera. However, feeding on rodents, mammals frequently found inside and around houses, represents further evidence related to the vector competence of L. intermedia, since synanthropic and sylvatic rodents have been considered a putative reservoir of L. (V. braziliensis.O teste de precipitina foi aplicado na identificação das fontes alimentares de Lutzomyia intermedia, de ambientes intra e peri-domiciliar, do Município de Mesquita, Estado do Rio de Janeiro, Brasil, área de transmissão de Leishmania (V. braziliensis. Foram testados os seguintes antisoros: homem, ave, cão, eqüino, gambá e roedor. De um total de 370 fêmeas analisadas, revelaram reatividade, contra os antisoros testados, 128 espécimes do intra-domicílio e 59 do ambiente peridomiciliar. A antropofilia de L. intermedia foi confirmada nos dois ambientes trabalhados; da mesma forma, a alimentação deste flebotomíneo em animais domésticos, observada anteriormente em levantamentos entomológicos, foi comprovada pela forte reatividade com antisoros de ave, cão e eqüino. Entretanto, a alimentação em roedores, animais sinantrópicos no ambiente domiciliar (interior e ao redor das residências, é mais uma forte evidência da competência vetorial de L. intermedia

  6. Traducción de significados en terapia familiar

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    Emilio Ricci

    2008-10-01

    Full Text Available Este artículo hace un reexamen parcial de algunos conceptos específicos, elaboraciones e ideas sobre la creación, efectiva, de la relación terapéutica, así como del enfoque de terapia familiar y de terapeuta “como estimulador de recursos” para activar de un modo directo a la familia y así producir elaboraciones y respuestas significativas, indispensable para hacer una elección terapéutica. ¿Cuáles son los aspectos que un terapeuta tendría que considerar para ser realmente auténtico en la relación terapéutica? ¿Cómo transformar la petición de ayuda terapéutica en un espacio de crecimiento para todo el sistema terapéutico (sistema familia – sistema terapeuta? ¿Qué se entiende con traducción de significados en terapia familiar? Estos aspectos son desarrollados siguiendo el enfoque relacional-sistémico.

  7. Terapia antihipertensiva en enfermedad renal crónica

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    V. Rodrigo Tagle, Dr.

    2010-07-01

    Full Text Available La enfermedad renal crónica (ERC constituye una de las causas más frecuentes de hipertensión arterial (HTA secundaria. Existe una nueva clasificación de la ERC basada en la presencia de un daño estructural con o sin un deterioro de la velocidad de filtración glomerular (VFG. La prevalencia de la HTA varía principalmente según la magnitud de la VFG y la etiología de la enfermedad del parénquima renal. Los mecanismos hipertensógenos primordiales son: la retención de sodio e hipervolemia, y la activación del eje renina angiotensina aldosterona. La HTA es también un factor de progresión de la ERC. La terapia se basa en la restricción de sodio, diuréticos y bloqueadores del eje renina angiotensina. Consideraciones farmacológicas y clínicas son fundamentales para un efectivo uso de esta terapia. Los objetivos son la prevención cardiovascular y renal, a través de la obtención de determinados niveles de presión arterial (PA.

  8. Occupational stress of workers in a public hospital of belo horizonte: a case study in intensive care units Estrés ocupacional de los trabajadores de un hospital público de belo horizonte: un estudio de caso en los centros de tratamiento intensivo Estresse ocupacional dos trabalhadores de um hospital público de belo horizonte: um estudo de caso nos centros de terapia intensiva

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    Maria de Lourdes Ulhôa

    2011-09-01

    Full Text Available This survey was conducted in 2009 to identify the main factors contributing to occupational stress of health workers in three adult ICUs of a public municipal hospital in Belo Horizonte. For this purpose a structured questionnaire was used with 28 questions taken from a version of the Job Stress Scale of the Demand-Control model of Karasek, abridged and adapted to Portuguese. This is a quantitative case study of a descriptive nature. One may consider that the objectives presented were achieved and that results indicated high levels of demand, control and social support. Correlation between the constructs showed no statistically significant differences for ICUs under study. Workers experience the work process actively and even when demands are excessive, they generate less harmful impacts because the worker can develop strategies to deal with difficulties of planning working hours according to his biological rhythms. The need to conduct research in the occupational health area is emphasized to develop and orient the hospital sectors together with the human resources sector to offer improved conditions for obtaining occupational health quality for these professionals.Esta investigación fue realizada en 2009 y tuvo como objetivo identificar los principales factores que contribuyen para el estrés ocupacional de los trabajadores de la salud en los tres CTIs adulto de un hospital público municipal de Belo Horizonte. Por ese motivo, se aplicó un cuestionario estructurado compuesto de 28 preguntas obtenidas de la versión resumida y adaptada para el portugués de la Job Stress Scale, proveniente del modelo de Demanda-Control de Karasek. Se trata de un estudio de caso cuantitativo de naturaleza descriptiva. Se puede considerar que los objetivos presentados fueron alcanzados y que los resultados indicaron altos niveles de Demanda, Control y Apoyo Social. La correlación entre los constructos no presenta diferencias estadísticamente significativas

  9. Eficacia y seguridad de la terapia tópica con capsaicina 0,075% versus mentol 1%, en el tratamiento del prurito de la foliculitis eosinofílica asociada al virus de la inmunodeficiencia adquirida

    OpenAIRE

    Galarza, Carlos; Instituto de Investigaciones Clínicas, Facultad de Medicina - Universidad Nacional Mayor de San Marcos. Lima, Perú. Servicio de Dermatología, Hospital Nacional Dos de Mayo. Lima, Perú. Servicio Académico Asistencial de Dermatología, Facultad deMedicina - Universidad Nacional Mayor de San Marcos. Lima, Perú; Ramos, Willy; Instituto de Investigaciones Clínicas, Facultad de Medicina - Universidad Nacional Mayor de San Marcos. Lima, Perú; Servicio Académico Asistencial de Dermatología, Facultad deMedicina - Universidad Nacional Mayor de San Marcos. Lima, Perú; Chía, Humberto; Servicio Académico Asistencial de Dermatología, Facultad deMedicina - Universidad Nacional Mayor de San Marcos. Lima, Perú; Ronceros, Gerardo; Instituto de Investigaciones Clínicas, Facultad de Medicina - Universidad Nacional Mayor de San Marcos. Lima, Perú; Santiani, Jesús; Instituto de Investigaciones Clínicas, Facultad de Medicina - Universidad Nacional Mayor de San Marcos. Lima, Perú; Gutiérrez, Ericson; Instituto de Investigaciones Clínicas, Facultad de Medicina - Universidad Nacional Mayor de San Marcos. Lima, Perú; Ñavincopa, Marcos; Instituto de Medicina Tropical Daniel Alcides Carrión, Facultad de Medicina - Universidad Nacional Mayor de San Marcos. Lima, Perú. Servicio de Enfermedades Infecciosas y Tropicales, Hospital Nacional Dos de Mayo. Lima, Perú; Vilcarromero, María; Instituto de Investigaciones Clínicas, Facultad de Medicina - Universidad Nacional Mayor de San Marcos. Lima, Perú; Ávila, Jack; Instituto de Investigaciones Clínicas, Facultad de Medicina - Universidad Nacional Mayor de San Marcos. Lima, Perú; Hancco, Jorge; Instituto de Investigaciones Clínicas, Facultad de Medicina - Universidad Nacional Mayor de San Marcos. Lima, Perú; Gámez, Deny; Clínica San Pablo. Lima, Perú

    2013-01-01

    Objetivo: Determinar la eficacia y seguridad de la terapia tópica con capsaicina 0,075% versus mentol 1% en el tratamiento del prurito de la foliculitis eosinofílica asociada al virus de la inmunodeficiencia adquirida (VIH). Diseño: Estudio clínico aleatorizado y doble ciego. Lugar: Servicios de Enfermedades Infecciosas y Tropicales (SEIT) y Dermatología, Hospital Nacional Dos de Mayo, Lima, Perú. Participantes: Pacientes con foliculitis eosinofílica asociada al VIH. Intervenciones: La muestr...

  10. Eficacia y seguridad de la terapia tópica con capsaicina 0,075% versus mentol 1%, en el tratamiento del prurito de la foliculitis eosinofílica asociada al virus de la inmunodeficiencia adquirida

    OpenAIRE

    Carlos Galarza; Willy Ramos; Humberto Chía; Gerardo Ronceros; Jesús Santiani; Ericson Gutiérrez; Marcos Ñavincopa; María Vilcarromero; Jack Ávila; Jorge Hancco; Deny Gámez

    2007-01-01

    Objetivo: Determinar la eficacia y seguridad de la terapia tópica con capsaicina 0,075% versus mentol 1% en el tratamiento del prurito de la foliculitis eosinofílica asociada al virus de la inmunodeficiencia adquirida (VIH). Diseño: Estudio clínico aleatorizado y doble ciego. Lugar: Servicios de Enfermedades Infecciosas y Tropicales (SEIT) y Dermatología, Hospital Nacional Dos de Mayo, Lima, Perú. Participantes: Pacientes con foliculitis eosinofílica asociada al VIH. Intervenciones: La muestr...

  11. Efecto de la aplicación de Terapia conductual en la adquisición de destrezas básicas para el aprendizaje, en tres niños con autismo.

    OpenAIRE

    Cabezas Pizarro, Hannia

    2004-01-01

    El presente estudio muestra el efecto de lautilización de terapia conductual aplicada, en tres ni-ños con autismo, y cuyo propósito fue el de desarrollaraquellas conductas de entrada básicas para el aprendizaje como son el permanecer sentados, el control dellanto, el establecimiento de contacto ocular y la identificación del yo, consideradas habilidades básicasque deben tener los niños para incursionar en el proceso educativo. Una vez que el niño adquiere esta gama de habilidades, estos reper...

  12. Valoración de la ingesta de nutrientes y energía en paciente crítico bajo terapia nutricional enteral Nutrients and energy intake assessment in the critically ill patient on enteral nutritional therapy

    OpenAIRE

    J. Abilés; G. Lobo; A. Pérez de la Cruz; M. Rodríguez; E. Aguayo; M. A. Cobo; R. Moreno-Torres; A. Aranda; J. Llopis; C. Sánchez; E. Planells

    2005-01-01

    Introducción y objetivos: El enfermo crítico es especialmente susceptible a la desnutrición debido a que su situación hipermetabólica conlleva a un aumento de los requerimientos nutricionales, que muchas veces no se cubren con el aporte de las fórmulas enterales suministradas. El estudio de la ingesta nutricional resulta imprescindible en este tipo de paciente para conocer en qué medida se cubren sus necesidades energético-nutricionales, mejorando y monitorizando la terapia clínico-nutriciona...

  13. Evaluación y tratamiento de terapia con ondas de choque piezoeléctricas en tendinopatías crónicas resistentes a tratamiento rehabilitador convencional

    OpenAIRE

    Ortega Moreno, Iván

    2016-01-01

    El manejo de las tendinopatías crónicas refractarias a otros tratamientos conservadores convencionales puede incluir la terapia con ondas de choque como una alternativa de tratamiento que mejora el dolor y la funcionalidad mediante un tratamiento eficaz no invasivo y con escasos efectos secundarios. Tras dos años de experiencia en el tratamiento de tendinopatías mediante ondas de choque con un generador de ondas piezoeléctricas en el Hospital del Henares, se propone un estudio prospectivo par...

  14. DESEMPEÑO DE UNA PELÍCULA DE MAÍZ AZUL EN EL ENVASADO DE UN QUESO DE HUMEDAD INTERMEDIA

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    CECILIA ROJAS DE G.

    Full Text Available La PCT/MX2009/000092, describe un método para obtener películas a partir de harina de cereales mediante método de "casting" y empleocomo almohadillas antimicrobianas yenvases termosellables para alimentos secos. Debido a su sensibilidad al agua y baja resistencia mecánica para contener alimentos de humedad intermedia; se reformuló la matriz biopolimérica para mejorar ambos parámetros.La película derivada de la reformulación fue caracterizada mecánicamente (ASTM D1708, se midió el "swelling" y se observó mediante microscopía electrónica de barrido.El desempeño de la película fue evaluado envasando unalimento perecedero: un quesosemimadurado,sometiéndolo a almacenamiento refrigerado durante 2 meses. Control: se emplearon queso del mismo lote envasado al vacío en película alta barrera. Durante los estudios de estabilidad en anaquel se evaluaron parámetros críticos microbiológicos como fisicoquímicos para el alimento envasado.Se observó que si bien las películas activas controlaron la carga microbiana, la integridad del envase se vio alterada debido a absorción de agua por contacto con el alimento, observándose ruptura de la estructura e integridad del biopolímeroy en consecuencia, exposición del producto y término de su vida útil.

  15. Correlation of male genital filaments and female spermathecal ducts in New World sand flies of the Lutzomyia intermedia species complex (Diptera: Psychodidae, Phlebotominae

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    Marcondes Carlos Brisola

    2003-01-01

    Full Text Available The lengths of the male genital filaments and female spermathecal ducts were measured in phlebotomine sand flies of the Lutzomyia intermedia species complex and the ratios between these characters calculated. Ratios for L. intermedia s. s. from Northeast vs Southeast Brazil (Espírito Santo and Minas Gerais, Espírito Santo/Minas Gerais vs Rio de Janeiro/São Paulo and L. intermedia vs L. neivai were significantly different at P < 0.1, 0.05 and 0.01 respectively when compared using ANOVA. The spermathecal ducts and genital filaments of L. intermedia were significantly longer than those of L. neivai (P < 0.01 and could be used to differentiate these species. The taxonomic and biological significance of these differences is discussed.

  16. Effect of caffeic acid phenethyl ester on Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages.

    Science.gov (United States)

    Choi, E-Y; Choe, S-H; Hyeon, J-Y; Choi, J-I; Choi, I S; Kim, S-J

    2015-12-01

    Caffeic acid phenethyl ester (CAPE) has numerous potentially beneficial properties, including antioxidant, immunomodulatory and anti-inflammatory activities. However, the effect of CAPE on periodontal disease has not been studied before. This study was designed to investigate the efficacy of CAPE in ameliorating the production of proinflammatory mediators in macrophages activated by lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen implicated in periodontal disease. LPS from P. intermedia ATCC 25611 was isolated by using the standard hot phenol-water method. Culture supernatants were assayed for nitric oxide (NO), interleukin (IL)-1β and IL-6. We used real-time polymerase chain reaction to quantify inducible NO synthase, IL-1β, IL-6, heme oxygenase (HO)-1 and suppressors of cytokine signaling (SOCS) 1 mRNA expression. HO-1 protein expression and levels of signaling proteins were assessed by immunoblot analysis. DNA-binding activities of NF-κB subunits were analyzed by using the enzyme-linked immunosorbent assay-based kits. CAPE exerted significant inhibitory effects on P. intermedia LPS-induced production of NO, IL-1β and IL-6 as well as their mRNA expression in RAW264.7 cells. CAPE-induced HO-1 expression in cells activated with P. intermedia LPS, and selective inhibition of HO-1 activity by tin protoporphyrin IX attenuated the inhibitory effect of CAPE on LPS-induced NO production. CAPE did not interfere with IκB-α degradation induced by P. intermedia LPS. Instead, CAPE decreased nuclear translocation of NF-κB p65 and p50 subunits induced with LPS, and lessened LPS-induced p50 binding activity. Further, CAPE showed strong inhibitory effects on LPS-induced signal transducer and activator of transcription 1 and 3 phosphorylation. Besides, CAPE significantly elevated SOCS1 mRNA expression in P. intermedia LPS-stimulated cells. Modulation of host response by CAPE may represent an attractive strategy towards the treatment of periodontal disease

  17. Situación de los Estudios Clínicos en Colombia

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    Alexander Carreño Dueñas

    2013-06-01

    Full Text Available Antecedentes: Los estudios clínicos son un tipo de investigación que ayudan a la ciencia médica a encontrar nuevas formas de prevenir, detectar, diagnosticar o tratar enfermedades y hoy en día son los estudios que aportan la mayor evidencia científica frente al uso de nuevas terapias farmacológicas. Objetivo: Describir la situación actual de los estudios clínicos en Colombia. Métodos: Se consultaron las bases de datos de registro de estudios clínicos, se realizó una revisión de los aspectos regulatorios y la normatividad relacionada. Resultados: no es posible determinar la cantidad exacta de estudios clínicos registrados en Colombia debido a que no se cuenta con un sistema de registro. Los estudios clínicos registrados difieren en las bases de datos consultadas. Desde 1995 a 2013 se identificaron 738 ensayos únicos, el 90% de ellos fueron patrocinados por industrias farmacéuticas, 62% fueron fase III y se han desarrollado principalmente en enfermedades crónicas como el cáncer, las enfermedades cardiovasculares y las degenerativas. Conclusiones: Colombia carece de un sistema de registro de estudios clínicos que permita realizar un seguimiento adecuado por parte de la comunidad y los profesionales de la salud. Debido a que el mayor porcentaje de estos estudios han sido patrocinados por la Industria Farmacéutica, se hace necesario fomentar y desarrollar estrategias que permitan el desarrollo de investigación propia

  18. Effect of nitric oxide-releasing derivative of indomethacin on Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages.

    Science.gov (United States)

    Choe, So-Hui; Choi, Eun-Young; Hyeon, Jin-Yi; Choi, In Soon; Kim, Sung-Jo

    2017-10-14

    The purpose of this study was to investigate the influences of NCX 2121, a nitric oxide (NO)-releasing derivative of indomethacin, upon the generation of proinflammatory mediators using murine macrophages activated by lipopolysaccharide (LPS) isolated from Prevotella intermedia, which is one of the pathogens implicated in periodontal diseases. Inducible NO synthase (iNOS)-derived NO, IL-1β and IL-6 as well as their relevant mRNA were significantly attenuated by NCX 2121 in RAW264.7 cells activated by P. intermedia LPS. NCX 2121 was much more effective than the parental compound indomethacin in reducing these proinflammatory mediators. NCX 2121 triggered induction of heme oxygenase-1 (HO-1) in cells exposed to P. intermedia LPS, and its inhibitory influence upon P. intermedia LPS-elicited NO generation was notably blocked by SnPP treatment. NCX 2121 attenuated NF-κB-dependent SEAP release induced by P. intermedia LPS. NCX 2121 did not display inhibitory action towards IκB-α degradation triggered by LPS. Instead, it significantly diminished nuclear translocation as well as DNA-binding action of NF-κB p50 subunit elicited by P. intermedia LPS. Further, NCX 2121 significantly up-regulated SOCS1 mRNA expression in cells challenged with P. intermedia LPS. In summary, NCX 2121 down-regulates P. intermedia LPS-elicited generation of NO, IL-1β and IL-6 in murine macrophages in a mechanism that involves anti-inflammatory HO-1 induction as well as decrement of NF-κB activation, which may be associated with SOCS1 expression. NCX 2121 may have potential benefits as a host immunomodulatory agent for the therapy of periodontal disease. Copyright © 2017 Elsevier Inc. All rights reserved.

  19. Prevotella intermedia stimulates tissue-type plasminogen activator and plasminogen activator inhibitor-2 expression via multiple signaling pathways in human periodontal ligament cells.

    Science.gov (United States)

    Guan, Su-Min; He, Jian-Jun; Zhang, Ming; Shu, Lei

    2011-06-01

    Prevotella intermedia is an important periodontal pathogen that induces various inflammatory and immune responses. In this study, we investigated the effects of P. intermedia on the plasminogen system in human periodontal ligament (hPDL) cells and explored the signaling pathways involved. Using semi-quantitative reverse transcription (RT)-PCR and quantitative real-time RT-qPCR, we demonstrated that P. intermedia challenge increased tissue-type plasminogen activator (tPA) and plasminogen activator inhibitor (PAI)-2 expression in a concentration- and time-dependent manner, but exerted no influence on urokinase-type plasminogen activator and PAI-1mRNA expression in hPDL cells. Prevotella intermedia stimulation also enhanced tPA protein secretion as confirmed by enzyme-linked immunosorbent assay. Western blot results revealed that P. intermedia treatment increased phosphorylation of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 kinase (p38). ERK, JNK and protein kinase C inhibitors significantly attenuated the P. intermedia-induced tPA and PAI-2 expression. Furthermore, p38 and phosphatidylinositol 3-kinase inhibitors markedly decreased PAI-2 expression, whereas they showed no or little inhibition on tPA expression. In contrast, inhibition of protein kinase A greatly enhanced the upregulatory effect of P. intermedia on tPA and PAI-2 expression. Our results suggest that P. intermedia may contribute to periodontal tissue destruction by upregulating tPA and PAI-2 expression in hPDL cells via multiple signaling pathways. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  20. Instrumento para habilitación de los servicios de terapia neural en Colombia

    OpenAIRE

    Huertas Pacheco, Oscar Mateo

    2013-01-01

    La Terapia Neural se basa en un modelo de atención integrativo que se realiza teniendo en cuenta la individualidad de cada paciente. Por esto, no es suficiente extrapolar los mismos estándares de la medicina convencional para formular estándares de habilitación. Se requiere entonces, superar el imaginario de que únicamente es una terapia infiltrativa de un anestésico local, y así, trascender a una terapia holística que aborda al ser humano. Utilizando el método Delphi se consultó, en suces...

  1. Avaliação clinica comparativa entre duas tecnicas de tratamento radicular na terapia periodontal

    OpenAIRE

    Lucio Flavio Teixeira Damis

    2000-01-01

    Resumo: Este estudo clínico teve como proposição comparar dois tipos de terapia para o tratamento da doença periodontal. A terapia tradicional que utiliza a raspagem e alisamento radicular com curetas manuais ­ grupo controle - e a terapia alternativa que propõe apenas destacar os cálculos usando curetas manuais e escovar a superficie radicular com solução iodada a 2% - grupo teste. Foram selecionados para cada grupo 40 faces de dentes unirradiculares com bolsas periodontais supra­ ósseas, co...

  2. La terapia breve: una alternativa de intervención psicológica efectiva

    OpenAIRE

    Hewitt Ramírez, Nohelia; Gantiva Díaz, Carlos Andrés

    2009-01-01

    El objetivo de este trabajo es describir y estructurar los orígenes, bases teóricas y los procedimientos de la terapia breve desde la perspectiva terapéutica cognitivo conductual. Se inicia con la identifi cación de las bases teóricas de la terapia breve, principalmente las teorías motivacionales relacionadas con la disponibilidad al cambio y la autoefi cacia desde la teoría social cognitiva. Se realiza un paralelo entre las características principales de la terapia breve en contraposición co...

  3. Terapias alternativas con animales para niños con necesidades especiales

    OpenAIRE

    Cea-Chueca, Aihnoa

    2014-01-01

    El presente trabajo de fin de grado consiste en la realización de un análisis y descripción de la literatura acerca de las distintas terapias animales, destinadas a niños con necesidades especiales: cómo y por qué surgieron, en qué consisten las terapias, cómo podemos utilizarlas, etc. Las terapias alternativas con animales se conocen como una alternativa terapéutica, donde se utiliza a un animal como co-terapeuta para poder desarrollar diferentes capacidades de los niños con necesidades espe...

  4. Terapias Psicológicas para el Tratamiento del Dolor Crónico

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    Jenny Moix

    2011-01-01

    Full Text Available A pesar de que existe abundante literatura que muestra la efectividad del tratamiento cognitivo-conductual del dolor crónico, en nuestro país, se carecía de un manual del mismo. Por este motivo, diseñamos un protocolo donde se describen las diferentes técnicas de dicha terapia. En el presente artículo, realizamos una detallada descripción del mismo. Antes de adentrarnos en la terapia cognitivo-conductual, presentamos una revisión de las principales terapias psicológicas que se emplean en el tratamiento del dolor crónico.

  5. Terapias Cognitiva e Cognitivo-Comportamental em dependência química

    OpenAIRE

    Silva,Cláudio Jerônimo da; Serra,Ana Maria

    2004-01-01

    Este artigo descreve o estado atual da Terapia Cognitiva, Comportamental, Prevenção de Recaída e Treinamento de Habilidades no tratamento de usuários de drogas. O objetivo é apresentar uma revisão sobre teorias e técnicas da Terapia Cognitiva e outras abordagens que dela derivam. Terapias Cognitiva e Comportamental, bem como Prevenção da Recaída e Treinamento de Habilidades, são tratamentos limitados no tempo, orientados em uma meta, e que utilizam sessões estruturadas, assumindo, assim, uma ...

  6. Aplicación de nuevas tecnologías en personas mayores con trastorno cognitivo leve-moderado desde la Terapia Ocupacional

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    María del Carmen Rodríguez Martínez

    2017-05-01

    Full Text Available Esta revisión bibliográfica pretende ofrecer una visión de las posibilidades que ofrecen las nuevas tecnologías a personas mayores con deterioro cognitivo leve-moderado para retardar o frenar los síntomas de deterioro cognitivo. Actualmente, las nuevas tecnologías de la información y la comunicación tienen mucho protagonismo y se han convertido en una herramienta con mucho potencial para este tipo de personas. Para ello, se han  analizado una serie de estudios sobre el uso de las nuevas tecnologías en personas mayores con trastornos cognitivos con el fin de ofrecer una visión más clara sobre los datos obtenidos hasta el día de hoy respecto a este campo de investigación en Terapia Ocupacional. Se seleccionaron un total de 204 artículos, tras un proceso de duplicación y tras pasar diferentes herramientas de calidad, quedaron un total de 7 artículos. Los resultados encontrados indican que las nuevas tecnologías contribuyen a disminuir algunos síntomas asociados a la enfermedad, como la ansiedad y la depresión, mejorando además ciertas funciones cognitivas en personas que tienen un deterioro cognitivo leve-moderado, siendo importante combinarlas con las terapias tradicionales.

  7. Mitogen-activated protein kinases and phosphatidylinositol 3-kinase are involved in Prevotella intermedia-induced proinflammatory cytokines expression in human periodontal ligament cells.

    Science.gov (United States)

    Guan, Su-Min; Zhang, Ming; He, Jian-Jun; Wu, Jun-Zheng

    2009-08-28

    Chronic periodontitis is an inflammatory disease affecting periodontal connective tissues and alveolar bone. Proinflammatory mediators induced by periodontal pathogens play vital roles in the initiation and progression of the disease. In this study, we examined whether Prevotella intermedia induces proinflammatory cytokines expression in human periodontal ligament cells (hPDLs). The mRNA expression and protein production were determined by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbant assay (ELISA) respectively. P. intermedia treatment dose- and time-dependently increased IL-6, IL-8 and M-CSF, but not IL-1beta and TNF-alpha mRNA expression and protein secretion. Preincubation of hPDLs with extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38 kinase and phosphatidylinositol 3-kinase (PI3K) inhibitors PD98059, SP600125, SB203580 and LY294002 resulted in significant reduction in P. intermedia-induced IL-6, IL-8 and M-CSF expression. Blocking the synthesis of prostaglandin E(2) (PGE(2)) by indomethacin also abolished the stimulatory effects of P. intermedia on cytokines expression. Our results indicate that P. intermedia induces proinflammatory cytokines through MAPKs and PI3K signaling pathways, and PGE(2) is involved in the P. intermedia-induced proinflammatory cytokines upregulation.

  8. Production of monoclonal antibodies capable of neutralizing dermonecrotic activity of Loxosceles intermedia spider venom and their use in a specific immunometric assay.

    Science.gov (United States)

    Alvarenga, Larissa M; Martins, Maria S; Moura, Juliana F; Kalapothakis, Evanguedes; Oliveira, Júlio C; Mangili, Oldemir C; Granier, Claude; Chávez-Olórtegui, Carlos

    2003-12-01

    We have produced 13 mAbs for Loxosceles intermedia crude venom. Twelve were reactive against proteins of 32-35 kDa and one of these Li mAb(7) showed high neutralizing potency for the dermonecrotic activity of L. intermedia venom. This Li mAb(7) showed no cross-reactivity, with Loxosceles laeta (Brazil), L. laeta (Perú) and Loxosceles gaucho venoms. The mAbs were produced by immunization with the crude venom and screened by enzyme-linked immunosorbent assay (ELISA) using L. intermedia whole venom or dermonecrotic fraction (DNF) as antigens coated onto microtitre plates. A sensitive two-site immunometric assay was designed and shown to be useful for identifying and quantifying DNF from L. intermedia in biological samples. The Li mAb(7) coated onto microtitre plates and hyperimmune horse anti-L. intermedia IgGs prepared by immunoaffinity chromatography and conjugated to horseradish peroxidase were used to set up a sandwich-type ELISA. Measurable absorbance signals were obtained with 0.2 ng of L. intermedia crude venom per assay.

  9. Terapia ocupacional e cultura: atravessamento, recurso ou campo de atuação? / Occupational Therapy and culture: crossing, resource or practice field?

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    Monica Villaça Gonçalves

    2017-11-01

    , protegiendotambiénsu diversidade. Para esta investigación, elegimosel enfoque cualitativo a partir de un enfoque analítico descriptivo, teniendo como base el método documentaldeldiario de campo de lasclases de los dos semestres consecutivos de la disciplina de Terapia Ocupacional Social de uncurso de una Universidad Publica. Los resultados obtenidos se dividieranentres categorias de análisis: (1 Cultura atravesandolapráctica, (2 Cultura como recurso y (3 Cultura como um campo de acción de la Terapia Ocupacional. Se entiende que la Cultura puede definir como campo específico de acción, con una finalidade determinada, lo que apunta para lanecesidad de los estúdios y uma formacción dirigida enparticular  aaquellas políticas, servicios y prácticas. Los datos de este estudio especialmente sumado a las diversas experienciasde la Terapia Ocupacional enelámbito de la Cultura han demonstrado lanecesidad de replantearlaformaciónprofesional. Una pista importante llevaría a uma revisión de lasdirectrices curriculares nacionales, teniendo em cuentaelámbito de la Cultura como locus de produccióndelconociento y laintervencióndel terapeuta ocupacional. Se apunta que és necesarioinvertiren neste tipo de formación para laconsolidación de lasprácticas de el terapeuta ocupacional enelámbito de la Cultura.Palalvras claves: Terapia Ocupacional, Cultura, Ciudadanía Cultural, Formación Profesional.

  10. Número de horas de cuidados de enfermagem em unidade de terapia intensiva de adultos Número de horas de cuidados de enfermería en unidad de terapia intensiva Number of nursing care hours in an intensive care unit

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    Ana Maria Tranquitelli

    2007-09-01

    Full Text Available O adequado dimensionamento de pessoal de enfermagem nas Terapias Intensivas tem sido uma grande preocupação para os enfermeiros que nelas atuam a despeito dos diferentes modelos de classificação dos pacientes propostos, no âmbito nacional e internacional. No presente estudo de caso, os objetivos foram estabelecer o tempo médio despendido na assistência direta de enfermagem em uma Unidade de Terapia Intensiva geral e calcular o número médio de horas de assistência direta de enfermagem prestada a esses pacientes. Para tanto, primeiramente, classificaram-se os procedimentos de enfermagem desenvolvidos com pacientes da UTI, de acordo com a sua complexidade: baixa, média e alta. A seguir, cronometrou-se o tempo médio despendido na realização desses procedimentos, a fim de se encontrar o tempo médio de assistência direta de enfermagem prestada aos pacientes, durante um período de três meses consecutivos.La dimensión adecuada del personal de enfermería en los centros de terapias intensivas ha sido una gran preocupación para los enfermeros que actúan en ellos, llevando en consideración los diferentes modelos de clasificación de los pacientes propuestos, tanto en el ámbito nacional como en el internacional. En este estudio, los objetivos fueron establecer el tiempo medio que se utilizó en la asistencia directa de enfermería en un centro de terapia intensiva (CTI general y calcular el número medio de horas de asistencia directa dispendidas con estos pacientes. Para eso fueron clasificados primeramente los procesos de enfermería desarrollados con pacientes de CTI, de acuerdo con su complejidad: baja, media y alta. Enseguida se cronometró el tiempo medio que se gastó en la realización de estos procedimientos con la finalidad de encontrar el tiempo medio de la atención de enfermería dedicada a los pacientes, durante un periodo de tiempo de tres meses consecutivos.The adequate sizing of the nursing staff in Intensive Care

  11. The production of 7-methyljuglone, plumbagin and quercetin in wild and cultivated Drosera rotundifolia and Drosera intermedia

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    B. Baranyai

    2016-09-01

    Full Text Available The recent establishment of Sphagnum farming areas has created large artificial habitats where Drosera grows under semi-natural conditions. Here we test the suitability, for pharmaceutical purposes, of two Drosera species collected from such areas. We measured the concentration of the biologically active compounds 7-methyljuglone, plumbagin and quercetin in Drosera rotundifolia and D. intermedia. All three compounds were found in pharmacologically suitable concentrations with 7-methlyjuglone characteristic for D. rotundifolia and plumbagin for D. intermedia. The concentrations required for pharmacological purposes were achieved within one year, but higher concentrations occurred in older plants and plants in flower. Concentrations did not differ between plants collected in the morning and in the afternoon. Drosera plants cultivated under semi-natural conditions are suitable as sources of raw materials for industrial pharmacological applications.

  12. Evaluation of Antioxidant Potential of Lavandula x intermedia Emeric ex Loisel. 'Budrovka': A Comparative Study with L. angustifolia Mill.

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    Maja Bival Štefan

    2010-08-01

    Full Text Available A Croatian indigenous cultivar of lavandin, Lavandula x intermedia 'Budrovka' (Lamiaceae was studied for the phenolic acids, flavonoids, anthocyanins, procyanidins and total tannins, as well as total polyphenols content, in the flower, inflorescence stalk and leaf ethanolic extracts. Antioxidant potentials on these plant part extracts were assessed by the DPPH free radical scavenging activity, iron chelating activity, reducing power, lipid peroxidation inhibition properties and total antioxidant capacity assays. All results were compared with those of Lavandula angustifolia, the only member of the Lavandula genus officially used in modern phytotherapy. Based on the results of our parallel study, we may suggest that Lavandula x intermedia 'Budrovka' is as potent an antioxidant as Lavandula angustifolia and the antioxidant activity of the Lavandula extracts is mainly due to the presence of rosmarinic acid. A good correlation was found between the polyphenol contents and antioxidant activities of the extracts

  13. Curcumin suppresses the production of interleukin-6 in Prevotella intermedia lipopolysaccharide-activated RAW 264.7 cells.

    Science.gov (United States)

    Kim, Sung-Jo

    2011-06-01

    Curcumin is known to exert numerous biological effects including anti-inflammatory activity. In this study, we investigated the effects of curcumin on the production of interleukin-6 (IL-6) by murine macrophage-like RAW 264.7 cells stimulated with lipopolysaccharide (LPS) from Prevotella intermedia, a major cause of inflammatory periodontal disease, and sought to determine the underlying mechanisms of action. LPS was prepared from lyophilized P. intermedia ATCC 25611 cells by the standard hot phenol-water method. Culture supernatants were collected and assayed for IL-6. We used real-time polymerase chain reaction to detect IL-6 mRNA expression. IκB-α degradation, nuclear translocation of NF-κB subunits, and STAT1 phosphorylation were characterized via immunoblotting. DNA-binding of NF-κB was also analyzed. Curcumin strongly suppressed the production of IL-6 at both gene transcription and translation levels in P. intermedia LPS-activated RAW 264.7 cells. Curcumin did not inhibit the degradation of IκB-α induced by P. intermedia LPS. Curcumin blocked NF-κB signaling through the inhibition of nuclear translocation of NF-κB p50 subunit. Curcumin also attenuated DNA binding activity of p50 and p65 subunits and suppressed STAT1 phosphorylation. Although further study is required to explore the detailed mechanism of action, curcumin may contribute to blockade of the host-destructive processes mediated by IL-6 and appears to have potential therapeutic values in the treatment of inflammatory periodontal disease.

  14. Simultaneous Detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA Gene-Directed Multiplex PCR

    Science.gov (United States)

    Conrads, Georg; Flemmig, Thomas F.; Seyfarth, Ilse; Lampert, Friedrich; Lütticken, Rudolf

    1999-01-01

    In a 16S rRNA gene-directed multiplex PCR, Prevotella intermedia- and Bacteroides forsythus-specific reverse primers were combined with a single conserved forward primer. A 660-bp fragment and an 840-bp fragment that were specific for both species could be amplified simultaneously. A total of 152 clinical samples, subgingival plaque and swabs of three different oral mucosae, from 38 periodontitis patients were used for the evaluation. PMID:10203541

  15. Comparative genome analysis of Prevotella intermedia strain isolated from infected root canal reveals features related to pathogenicity and adaptation

    OpenAIRE

    Ruan, Yunfeng; Shen, Lu; Zou, Yan; Qi, Zhengnan; Yin, Jun; Jiang, Jie; Guo, Liang; He, Lin; Chen, Zijiang; Tang, Zisheng; Qin, Shengying

    2015-01-01

    Background Many species of the genus Prevotella are pathogens that cause oral diseases. Prevotella intermedia is known to cause various oral disorders e.g. periodontal disease, periapical periodontitis and noma as well as colonize in the respiratory tract and be associated with cystic fibrosis and chronic bronchitis. It is of clinical significance to identify the main drive of its various adaptation and pathogenicity. In order to explore the intra-species genetic differences among strains of ...

  16. Prevenzione delle infezioni ospedaliere in una terapia intensiva

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    G.B. Orsi

    2003-05-01

    Full Text Available

    Obiettivi: sorveglianza delle infezioni ospedaliere (IO nell’unità di terapia intensiva (UTI di un grande ospedale romano.

    Metodi. tutti i pazienti ricoverati nell’UTI tra il 1.1.2000 ed il 31.12.2001 per ›48 ore. Lo studio, applicando le definizioni di caso dei CDC, ha considerato le seguenti infezioni: infezioni polmonari (POL, setticemie (SET, infezioni delle vie urinarie (IVU, infezioni della ferita chirurgica (IFC. Inoltre sono stati valutati eventuali fattori di rischio preesistenti, procedure invasive, isolamento dei microrganismi e loro suscettibilità agli antibiotici.

    Sulla base dei risultati epidemiologici preliminari, dall’ottobre 2000 al marzo 2001 sono stati effettuati una serie di interventi che hanno modificato alcune importanti variabili associate alle IO.

  17. Tratamiento de Terapia Ocupacional en el accidente cerebrovascular

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    Domingo García, AM

    2006-02-01

    Full Text Available RESUMENEntre los muchos pacientes que necesitan tratamiento rehabilitador en Terapia Ocupacional están los que en la edad adulta han sufrido un accidente cerebrovascular.Uno de los factores de riesgo asociados con más frecuencia a las alteraciones del sistema nervioso central es el progresivo envejecimiento de la población, debido a esto orientaré el siguiente artículo hacia la intervención sobre la población geriátrica.La Terapia Ocupacional ofrece un tratamiento global que abarca las áreas funcional, motriz, sensorial, perceptivo y cognitiva. No debemos olvidar el asesoramiento realizado al paciente, a la familia y/ o cuidadores sobre el manejo de la persona que ha sufrido un ictus, la adaptación del entorno y la prescripción, uso y manejo de ayudas técnicas cuando sea necesario.Las propuestas terapéuticas que expondré a continuación, no son recetas únicas que se puedan emplear del mismo modo en todos las personas que hayan sufrido un ictus. Con cada paciente será necesario una evaluación individualizada de su situación y una adaptación de la terapia según sus déficit específicos.La meta final de la Terapia Ocupacional es la integración óptima del paciente dentro de su entorno familiar y social, con el mayor grado de autonomía posible.ABSTRACTAmong the patients who need Occupational Therapy’s rehabilitation treatment, there are those who have suffered a cerebrovascular damage when elderly.People’s gradual ageing is one of the risk factors in the nervous central system’s alterations and this is why I am going to write about intervention on geriatric population.The Occupational Therapy’s rehabilitation treatment works on the functional, motor, sensorial, perceptive and cognitive areas. Moreover, we should not forget to advise the patient and his/her family or caregivers about the way of treating ,the environment’s adaptation, the prescription and use of technical aids when needed.The following therapeutic

  18. Biocidal Potential and Chemical Composition of Industrial Essential Oils from Hyssopus officinalis, Lavandula × intermedia var. Super, and Santolina chamaecyparissus.

    Science.gov (United States)

    Ortiz de Elguea-Culebras, Gonzalo; Sánchez-Vioque, Raúl; Berruga, María Isabel; Herraiz-Peñalver, David; González-Coloma, Azucena; Andrés, María Fé; Santana-Méridas, Omar

    2018-01-01

    This work presents the biocidal (insecticidal, ixodicidal, nematicidal, and phytotoxic) effects and chemical compositions of three essential oils obtained from the industrial steam distillation (IEOs) of hyssop (Hyssopus officinalis L.), lavandin (Lavandula × intermedia or L. × hybrida var. Super), and cotton lavender (Santolina chamaecyparissus L.). Their chemical composition analyzed by gas chromatography coupled to mass spectrometry showed 1,8-cineole (53%) and β-pinene (16%) as the major components of H. officinalis, linalyl acetate (38%) and linalool (29%) of L. × intermedia; and 1,8-cineole (10%) and 8-methylene-3-oxatricyclo[5.2.0.0 2,4 ]nonane (8%) in S. chamaecyparissus. The biocidal tests showed that L. × intermedia IEO was the most active against the insect Spodoptera littoralis and toxic to the tick Hyalomma lusitanicum, IEO of H. officinalis was strongly active against S. littoralis, and finally, S. chamaecyparissus IEO was a strong antifeedant against the aphid Rhopalosiphum padi, toxic to H. lusitanicum and with moderate effects against Leptinotarsa decemlineata, S. littoralis, and Lolium perenne. © 2018 Wiley-VHCA AG, Zurich, Switzerland.

  19. Prevotella intermedia induces severe bacteremic pneumococcal pneumonia in mice with upregulated platelet-activating factor receptor expression.

    Science.gov (United States)

    Nagaoka, Kentaro; Yanagihara, Katsunori; Morinaga, Yoshitomo; Nakamura, Shigeki; Harada, Tatsuhiko; Hasegawa, Hiroo; Izumikawa, Koichi; Ishimatsu, Yuji; Kakeya, Hiroshi; Nishimura, Masaharu; Kohno, Shigeru

    2014-02-01

    Streptococcus pneumoniae is the leading cause of respiratory infection worldwide. Although oral hygiene has been considered a risk factor for developing pneumonia, the relationship between oral bacteria and pneumococcal infection is unknown. In this study, we examined the synergic effects of Prevotella intermedia, a major periodontopathic bacterium, on pneumococcal pneumonia. The synergic effects of the supernatant of P. intermedia (PiSup) on pneumococcal pneumonia were investigated in mice, and the stimulation of pneumococcal adhesion to human alveolar (A549) cells by PiSup was assessed. The effects of PiSup on platelet-activating factor receptor (PAFR) transcript levels in vitro and in vivo were analyzed by quantitative real-time PCR, and the differences between the effects of pneumococcal infection induced by various periodontopathic bacterial species were verified in mice. Mice inoculated with S. pneumoniae plus PiSup exhibited a significantly lower survival rate, higher bacterial loads in the lungs, spleen, and blood, and higher inflammatory cytokine levels in the bronchoalveolar lavage fluid (macrophage inflammatory protein 2 and tumor necrosis factor alpha) than those infected without PiSup. In A549 cells, PiSup increased pneumococcal adhesion and PAFR transcript levels. PiSup also increased lung PAFR transcript levels in mice. Similar effects were not observed in the supernatants of Porphyromonas gingivalis or Fusobacterium nucleatum. Thus, P. intermedia has the potential to induce severe bacteremic pneumococcal pneumonia with enhanced pneumococcal adhesion to lower airway cells.

  20. The influence of molecular oxygen exposure on the biology of Prevotella intermedia, with emphasis on its antibiotic susceptibility.

    Science.gov (United States)

    dos Santos, S G; Diniz, C G; da Silva, V L; Souza, N C; de Lima, F L; Bomfim, M R Q; de Carvalho, M A R; Farias, L M

    2007-10-01

    This study focuses on investigating the molecular and physiological characteristics of Prevotella intermedia after molecular oxygen exposure (MOE) and the effect on drug susceptibility patterns. Samples of P. intermedia were used as parent strains: ATCC25611 and four clinical isolates. Strains adapted to oxidative stress by MOS were obtained by the enrichment technique. Drug susceptibility was evaluated by minimal inhibitory concentrations (MIC) using agar dilution. Arbitrarily primed-polymerase chain reaction (AP-PCR) was used to evaluate the genetic diversity of all strains and physiological analyses were made by sodiumdodecylsulfate-polyacrylamide gel electrophoresis and two-dimensional electrophoresis of crude, cell-free extracts. The genetic profile showed that lineages with altered MIC values were selected after MOE. Overall, we found significant decrease in drug susceptibility for the aero-strains against all tested antimicrobials (amoxicillin, amoxicillin+clavulanic acid, clindamycin, chloramphenicol, ertapenen and metronidazole). We also observed markedly different protein expression patterns between the parent and selected aero-strains. MOE induces changes in the genetic profile and protein expression patterns of P. intermedia that may also be linked to its drug resistance mechanisms. The effects of MOE on anaerobic bacterial physiology and behaviour may influence antimicrobial susceptibility patterns with potential consequences to antimicrobial chemotherapy.

  1. Human dental stem cells suppress PMN activity after infection with the periodontopathogens Prevotella intermedia and Tannerella forsythia.

    Science.gov (United States)

    Hieke, Cathleen; Kriebel, Katja; Engelmann, Robby; Müller-Hilke, Brigitte; Lang, Hermann; Kreikemeyer, Bernd

    2016-12-15

    Periodontitis is characterized by inflammation associated with the colonization of different oral pathogens. We here aimed to investigate how bacteria and host cells shape their environment in order to limit inflammation and tissue damage in the presence of the pathogen. Human dental follicle stem cells (hDFSCs) were co-cultured with gram-negative P. intermedia and T. forsythia and were quantified for adherence and internalization as well as migration and interleukin secretion. To delineate hDFSC-specific effects, gingival epithelial cells (Ca9-22) were used as controls. Direct effects of hDFSCs on neutrophils (PMN) after interaction with bacteria were analyzed via chemotactic attraction, phagocytic activity and NET formation. We show that P. intermedia and T. forsythia adhere to and internalize into hDFSCs. This infection decreased the migratory capacity of the hDFSCs by 50%, did not disturb hDFSC differentiation potential and provoked an increase in IL-6 and IL-8 secretion while leaving IL-10 levels unaltered. These environmental modulations correlated with reduced PMN chemotaxis, phagocytic activity and NET formation. Our results suggest that P. intermedia and T. forsythia infected hDFSCs maintain their stem cell functionality, reduce PMN-induced tissue and bone degradation via suppression of PMN-activity, and at the same time allow for the survival of the oral pathogens.

  2. [Effects of cutting and reseeding on the ground-dwelling arthropod community in Caragana intermedia forest in desert steppe].

    Science.gov (United States)

    Liu, Ren-Tao; Chai, Yong-Qing; Yang, Xin-Guo; Song, Nai-Ping; Wang, Xin-Yun; Wang, Lei

    2013-01-01

    Taking a 25-year-old Caragana intermedia forest in desert steppe as test object, an investigation was conducted on the ground-dwelling arthropod community in cutting and no-cutting stands with and without reseeding, aimed to understand the effects of cutting, reseeding and their interaction on the individual number and group richness of ground-dwelling arthropod in C. intermedia forest. There were significantly lower number and richness of ground-dwelling arthropod in the open spaces than under the shrubs in the no-cutting and no-reseeding stands. Cutting, reseeding and both of them could significantly increase the number and richness of ground-dwelling arthropod in the open spaces, but not under the shrubs, compared with no cutting or reseeding. Consequently, there were no significant differences in the distribution of ground-dwelling arthropod in the open spaces and under the shrubs in the cutting, reseeding, or cutting and reseeding stands. Further, there was a similar buffer effect between cutting and reseeding on the ground-dwelling arthropod. No significant differences were observed in the ground-dwelling arthropod distribution, between cutting stand and reseeding stand, between cutting stand and cutting and reseeding stand, and between reseeding stand and cutting and reseeding stand. It was suggested that cutting, reseeding, or both of them could significantly improve the ground-dwelling arthropod diversity especially in the open spaces, being beneficial for the restoration of degraded grassland ecosystem and the rational management on artificial C. intermedia forest in desert steppe.

  3. Descrição do comportamento agonístico de Loxosceles laeta, L. hirsuta e L. intermedia (Araneae: Sicariidae Description of the agonistic behaviors of Loxosceles laeta, L. hirsuta, and L. intermedia (Araneae: Sicariidae

    Directory of Open Access Journals (Sweden)

    Marta L. Fischer

    2008-12-01

    Full Text Available Os grupos de radiação evolutiva de Loxosceles Heinecken & Lowe, 1835 além de compartilharem caracteres morfológicos aparentemente também apresentam comportamentos semelhantes. Assim, o presente estudo objetivou descrever o comportamento agonístico dos machos das espécies do grupo spadicea - Loxosceles intermedia Mello-Leitão, 1934 e Loxosceles hirsuta Mello-Leitão, 1931 - e laeta -Loxosceles laeta (Nicolet 1849 - ocorrentes no estado do Paraná. Para tal, os machos foram pareados, sendo o invasor colocado nas teias do residente, descritos e quantificados os padrões motores exibidos. O comportamento agonístico de machos de L. laeta, L. intermedia e L. hirsuta foi caracterizado pelo afrontamento, defesa e combate, sendo apenas o último ritualizado e relacionado com peculiaridades de cada espécie, tendo em comum a sinalização de tamanho e força. Além dos padrões motores exibidos durante o comportamento agonístico serem semelhantes com aqueles utilizados no comportamento sexual, os padrões motores de L. intermedia e L. hirsuta foram mais semelhantes entre si e diferentes de L. laeta, sugerindo relação com o grupo de radiação evolutiva.The evolutionary groups of species of Loxosceles Heinecken & Lowe, 1835, besides having morphological characters in common, may also show similar behaviors. The present study describes the agonistic behavior of males of members of the spadicea group - Loxosceles intermedia Mello-Leitão, 1934 and L hirsuta Mello-Leitão, 1931 - and the laeta-group - L. laeta (Nicolet, 1849 - that occur in the state of Paraná. In experiments with paired males, an intruder male was placed in the web of a resident male web, and the motor patterns displayed were described and quantified. Males of all three species showed confrontation, defense and combat behaviors. The combat was ritualized and related to peculiarities of each species, while sharing the signaling of strength and size. The motor patterns shown

  4. Efeitos da terapia física descongestiva na cicatrização de úlceras venosas Efectos de la terapia física descongestiva en la cicatrización de úlceras venosas Effects of the decongestive physiotherapy in the healing of venous ulcers

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    Roberta Azoubel

    2010-12-01

    Full Text Available Objetivou-se neste estudo verificar os efeitos da terapia física descongestiva (TFD na cicatrização de úlceras venosas. Trata-se de um estudo intervencionista, quase experimental, do qual participaram 20 clientes, divididos em 2 grupos: o grupo controle (n=10 e o grupo de intervenção (n=10. Os clientes do primeiro grupo foram tratados apenas com curativo convencional e os do segundo grupo, com curativo convencional e terapia física descongestiva (associação de técnicas: drenagem linfática manual, enfaixamento compressivo, elevação dos membros inferiores, exercícios miolinfocinéticos e cuidados com a pele. Ambos os grupos foram tratados durante seis meses. Os clientes submetidos à TFD apresentaram significante redução de edema e da dor, além de melhora no processo cicatricial. Os resultados permitiram verificar que a terapia descongestiva estimula o processo de cicatrização de úlceras venosas, melhorando a qualidade de vida dos indivíduos.En este estudio se objetivó verificar los efectos de la terapia física descongestiva (TFD en la cicatrización de úlceras venosas. Se trató de un estudio intervencionista, casi experimental, del cual participaron veinte pacientes que constituyeron dos grupos: el grupo control (n=10 y el grupo de intervención (n=10. Los pacientes del primer grupo fueron tratados apenas con curaciones convencionales, mientras que los del segundo grupo recibieron curación convencional y terapia física descongestiva (asociación de técnicas: drenaje linfático manual, fajamiento compresivo, elevación de los miembros inferiores, ejercicios miolinfocinéticos y cuidados con la piel. Ambos grupos fueron tratados durante seis meses. Los pacientes sometidos a TFD presentaron significativa reducción de edema y dolor, y mejora en el proceso cicatricial. Los resultados permitieron verificar que la terapia descongestiva estimula el proceso de cicatrización de úlceras venosas, mejorando la calidad de vida de

  5. Assessment of phlebitis, infiltration and extravasation events in neonates submitted to intravenous therapy Avaliacion de la ocurrencia de flebitis, infiltración y extravasamiento en neonatos submetidos a terapia intravenosa Avaliação da ocorrência de flebite, infiltração e extravasamento em neonatos submetidos à terapia intravenosa

    Directory of Open Access Journals (Sweden)

    Ana Caroline Rodrigues Gomes

    2011-09-01

    Full Text Available This study aimed to describe phlebitis, infiltration and extravasation events in newborn infants hospitalized at the neonatal intensive care unit of a public maternity in Rio de Janeiro, Brazil. A quantitative and descriptive study was carried out, involving 36 newborns under intravenous therapy and indicated for the removal of the peripheral intravenous device. Fifty puncture sites were assessed immediately after the peripheral catheter removal, resulting in an average 1.40 punctures per infant. Complications were responsible for 48% of catheter removals before discharge from treatment, predominantly infiltration (79.2%, followed by phlebitis (16.7% and extravasation (4.2%. To avoid aggravations and enhance the security of newborns submitted to intravenous therapy, the nursing team should periodically assess the peripheral venous access and gain knowledge on interventions needed when signs of complications are detected.El objetivo de este estudio fue describir la ocurrencia de flebitis, infiltración y extravasamiento en recién nacidos internados en la unidad de terapia intensiva neonatal de una maternidad pública de Rio de Janeiro. Se trata de un estudio cuantitativo descriptivo con 36 recién nacidos en uso de terapia intravenosa y con indicación de remoción del dispositivo intravenoso periférico. Fueron evaluados 50 sitios de punción inmediatamente después de la remoción de catéteres periféricos originando una media de 1,40 punciones venosas por neonato. Las complicaciones fueron responsables por 48% de las remociones de los catéteres, antes del alta del tratamiento, con predominio de infiltraciones (79,2%, seguida por flebitis (16,7% e extravasamiento (4,2%. Con el fin de evitar los lesiones y promover la seguridad de los recién nacidos sometidos a terapia intravenosa, el equipo de enfermería debe evaluar periódicamente el acceso venoso periférico y obtener conocimiento acerca de las intervenciones necesarias cuando

  6. Fungal His-tagged nitrilase from Gibberella intermedia: gene cloning, heterologous expression and biochemical properties.

    Science.gov (United States)

    Gong, Jin-Song; Li, Heng; Zhu, Xiao-Yan; Lu, Zhen-Ming; Wu, Yan; Shi, Jing-Song; Xu, Zheng-Hong

    2012-01-01

    Nitrilase is an important member of the nitrilase superfamiliy. It has attracted substantial interest from academia and industry for its function of converting nitriles directly into the corresponding carboxylic acids in recent years. Thus nitrilase has played a crucial role in production of commercial carboxylic acids in chemical industry and detoxification of nitrile-contaminated wastes. However, conventional studies mainly focused on the bacterial nitrilase and the potential of fungal nitrilase has been far from being fully explored. Research on fungal nitrilase gene expression will advance our understanding for its biological function of fungal nitrilase in nitrile hydrolysis. A fungal nitrilase gene from Gibberella intermedia was cloned through reverse transcription-PCR. The open reading frame consisted of 963 bp and potentially encoded a protein of 320 amino acid residues with a theoretical molecular mass of 35.94 kDa. Furthermore, the catalytic triad (Glu-45, Lys-127, and Cys-162) was proposed and confirmed by site-directed mutagenesis. The encoding gene was expressed in Escherichia coli Rosetta-gami (DE3) and the recombinant protein with His(6)-tag was purified to electrophoretic homogeneity. The purified enzyme exhibited optimal activity at 45°C and pH 7.8. This nitrilase was specific towards aliphatic and aromatic nitriles. The kinetic parameters V(max) and K(m) for 3-cyanopyridine were determined to be 0.81 µmol/min·mg and 12.11 mM through Hanes-Woolf plot, respectively. 3-Cyanopyridine (100 mM) could be thoroughly hydrolyzed into nicotinic acid within 10 min using the recombinant strain with the release of about 3% nicotinamide and no substrate was detected. In the present study, a fungal nitrilase was cloned from the cDNA sequence of G. intermedia and successfully expressed in E. coli Rosetta-gami (DE3). The recombinant strain displayed good 3-cyanopyridine degradation efficiency and wide substrate spectrum. This fungal nitrilase might be a

  7. Fungal His-tagged nitrilase from Gibberella intermedia: gene cloning, heterologous expression and biochemical properties.

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    Jin-Song Gong

    Full Text Available Nitrilase is an important member of the nitrilase superfamiliy. It has attracted substantial interest from academia and industry for its function of converting nitriles directly into the corresponding carboxylic acids in recent years. Thus nitrilase has played a crucial role in production of commercial carboxylic acids in chemical industry and detoxification of nitrile-contaminated wastes. However, conventional studies mainly focused on the bacterial nitrilase and the potential of fungal nitrilase has been far from being fully explored. Research on fungal nitrilase gene expression will advance our understanding for its biological function of fungal nitrilase in nitrile hydrolysis.A fungal nitrilase gene from Gibberella intermedia was cloned through reverse transcription-PCR. The open reading frame consisted of 963 bp and potentially encoded a protein of 320 amino acid residues with a theoretical molecular mass of 35.94 kDa. Furthermore, the catalytic triad (Glu-45, Lys-127, and Cys-162 was proposed and confirmed by site-directed mutagenesis. The encoding gene was expressed in Escherichia coli Rosetta-gami (DE3 and the recombinant protein with His(6-tag was purified to electrophoretic homogeneity. The purified enzyme exhibited optimal activity at 45°C and pH 7.8. This nitrilase was specific towards aliphatic and aromatic nitriles. The kinetic parameters V(max and K(m for 3-cyanopyridine were determined to be 0.81 µmol/min·mg and 12.11 mM through Hanes-Woolf plot, respectively. 3-Cyanopyridine (100 mM could be thoroughly hydrolyzed into nicotinic acid within 10 min using the recombinant strain with the release of about 3% nicotinamide and no substrate was detected.In the present study, a fungal nitrilase was cloned from the cDNA sequence of G. intermedia and successfully expressed in E. coli Rosetta-gami (DE3. The recombinant strain displayed good 3-cyanopyridine degradation efficiency and wide substrate spectrum. This fungal nitrilase might be

  8. Estenosis traqueal: Estudio preliminar

    OpenAIRE

    Rafael Pinilla González; Asbel Vicente de la Cruz

    2000-01-01

    Se realizó un estudio retrospectivo y descriptivo con la utilización de los expedientes clínicos de 5 pacientes con estenosis traqueal, mayoritariamente como consecuencia de intubación prolongada, y en el que se tuvo en cuenta aspectos relacionados con el diagnóstico, el tratamiento y las complicaciones. Existió un predominio del sexo femenino y edad media de 44,6 años. La intubación prolongada de origen diverso fue la causa de la estenosis en 4 de los 5 pacientes y en 1 fue posterior a la re...

  9. Efecto de la terapia física y terapia ocupacional en una paciente con Síndrome de West: Reporte de un caso

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    Herminio Teófilo Camacho-Conchucos

    2014-10-01

    Full Text Available Se presenta el caso de una niña de 12 años con diagnóstico de Síndrome de West. Asistió a consulta médica por primera vez a los 8 meses de edad, sus convulsiones se acentuaron a la edad de dos años y medio, lo que agravó el retraso psicomotor. Inicio terapia física y terapia ocupacional, empleando técnicas de neurodesarrollo y marcos de referencia respectivamente. A la presentación del caso se observaron avances favorables en aspectos cualitativos más que cuantitativos.

  10. Transferencia génica asistida por campos magnéticos: estudios en células gliales.

    OpenAIRE

    Pardo, Joaquin; Sosa, Yolanda Elena; Reggiani, Paula Cecilia; Arciniegas, M. I.; Sanchez, Francisco Homero; Goya, Rodolfo Gustavo

    2015-01-01

    Las nanopartículas magnéticas (MNP) complejadas con vectores génicos pueden, en presencia de un campo magnético externo, amplificar sustancialmente la eficiencia de la transferencia génica. Esta técnica, denominada magnetofección, es de gran interés en el campo de la terapia génica. En este estudio se caracterizó la mejora de transferencia génica en células gliales B92 utilizando complejos constituidos por diferentes proporciones de...

  11. Biosynthesis of silver nanoparticles using Euglena gracilis, Euglena intermedia and their extract.

    Science.gov (United States)

    Li, Yong; Tang, Xiaoling; Song, Wenshuang; Zhu, Lina; Liu, Xingang; Yan, Xiaomin; Jin, Chengzhi; Ren, Qingguang

    2015-02-01

    Extracellular and intracellular biosynthesis of silver nanoparticles (AgNPs) by Euglena gracilis (EG) strain and Euglena intermedia (EI) strain are reported in this study. The obtained nanoparticles showed an absorption peak approximates 420 nm in the UV-visible spectrum, corresponding to the plasmon resonance of AgNPs. According to the result of inductively coupled plasma-atomic emission spectrometer, the intakes of silver ions by EI and EG are roughly equal. The transmission electron microscope (TEM) analysis of the successful in vivo and in vitro synthesised AgNPs indicated the sizes, ranging from 6 to 24 nm and 15 to 60 nm in diameter, respectively, and a spherical-shaped polydispersal of the particles. The successful formation of AgNPs has been confirmed by energy dispersive X-ray analysis connected to the TEM. The Fourier transform infrared spectroscopy measurements reveal the presence of bioactive functional groups such as amines are found to be the capping and stabilising agents of nanoparticles. To our knowledge, this is the first report where two kinds of Euglena microalga were used as the potential source for in vivo and in vitro biosynthesis of AgNPs.

  12. POLÍTICAS URBANAS PARA EL DESARROLLO Y LA INNOVACIÓN EN LAS CIUDADES INTERMEDIAS

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    Henar Pascual Ruiz-Valdepeñas

    2008-01-01

    Full Text Available En los últimos años se asiste al fortalecimiento del papel de las ciudades intermedias al estimarsesu capacidad para lograr una mayor cohesión económica, social y territorial en Europa. Las políticas territoriales se orientan a reforzar sus ventajas competitivas y a potenciar la configuración y consolidación de redes de cooperación en las que las ciudades actúan como partes de un modelo policéntrico. La valoración de los impactos de los programas europeos en las ciudades y el análisis de las políticas y estrategias urbanas, ponen de relieve la naturaleza específica de los diversos niveles de intervención pública en el territorio y la multiplicidad de las interacciones que se generan entre ellos. En el nivel local de intervención, la planificación estratégica amplía el abanico de objetivos y actuaciones y aporta nuevos instrumentos que ponen el acento en el reforzamiento de los factores de competitividad urbana.

  13. Functional morphology of the reproductive system of Galathea intermedia (Decapoda: Anomura).

    Science.gov (United States)

    Kronenberger, Katrin; Brandis, Dirk; Türkay, Michael; Storch, Volker

    2004-10-01

    Spermatophore formation in Galathea intermedia begins in the proximal part of the vas deferens. The contents subsequently form a spermatophoric ribbon, the so-called "secondary spermatophore," in its distal part. A strongly muscular ductus ejaculatorius is present in the coxa of the fifth pereiopod which builds up pressure for the extrusion of the spermatophoric ribbon. After extrusion, the ribbon is caught by the first gonopod, while the second gonopod dissolves the matrix of the ribbon. During copulation the spermatophores are randomly placed on the sternum of the female, near the genital opening, by the fifth pereiopods of the male. Subsequent ovulation of the female via the genital opening, an active process accomplished through muscular activity, results in fertilization of the eggs by the exploding spermatophores. External intersexes are characterized by both male and female external sexual characters, but in all individuals only male gonads are present. No trace of a female reproductive system could be detected. Thus, these external intersexes are exclusively functional males. 2004 Wiley-Liss, Inc.

  14. Improving the catalytic potential and substrate tolerance of Gibberella intermedia nitrilase by whole-cell immobilization.

    Science.gov (United States)

    Li, Heng; Yang, Tao; Gong, Jin-Song; Xiong, Lei; Lu, Zhen-Ming; Li, Hui; Shi, Jin-Song; Xu, Zheng-Hong

    2015-01-01

    Comparative studies of immobilized and free cells of Gibberella intermedia CA3-1 in bioconversion of 3-cyanopyridine to nicotinic acid were performed. Entrapping method was chosen based on the advantages in enzymatic activity recovery, mechanical strength and preparation procedure. Four entrapment matrices were investigated and sodium alginate was screened to be the most suitable material. Maximal nitrilase activity of alginate immobilized cells was obtained under conditions of 2 % alginate, 0.6 % CaCl2, 0.4 g cell/g alginate, 1.8 mm bead size. The immobilized cells showed excellent substrate tolerance even when the 3-cyanopyridine concentration was 700 mM. The half-lives of immobilized cells at 30, 40 and 50 °C were 315, 117.5 and 10.9 h, respectively, correspondingly 1.4, 1.6 and 1.7-fold compared with that of the free cells. Efficient reusability of immobilized cells up to 28 batches was achieved and 205.7 g/(g dcw) nicotinic acid was obtained with 80.55 % enzyme activity preserved.

  15. A new autocatalytic activation mechanism for cysteine proteases revealed by Prevotella intermedia interpain A.

    Science.gov (United States)

    Mallorquí-Fernández, Noemí; Manandhar, Surya P; Mallorquí-Fernández, Goretti; Usón, Isabel; Wawrzonek, Katarzyna; Kantyka, Tomasz; Solà, Maria; Thøgersen, Ida B; Enghild, Jan J; Potempa, Jan; Gomis-Rüth, F Xavier

    2008-02-01

    Prevotella intermedia is a major periodontopathogen contributing to human gingivitis and periodontitis. Such pathogens release proteases as virulence factors that cause deterrence of host defenses and tissue destruction. A new cysteine protease from the cysteine-histidine-dyad class, interpain A, was studied in its zymogenic and self-processed mature forms. The latter consists of a bivalved moiety made up by two subdomains. In the structure of a catalytic cysteine-to-alanine zymogen variant, the right subdomain interacts with an unusual prodomain, thus contributing to latency. Unlike the catalytic cysteine residue, already in its competent conformation in the zymogen, the catalytic histidine is swung out from its active conformation and trapped in a cage shaped by a backing helix, a zymogenic hairpin, and a latency flap in the zymogen. Dramatic rearrangement of up to 20A of these elements triggered by a tryptophan switch occurs during activation and accounts for a new activation mechanism for proteolytic enzymes. These findings can be extrapolated to related potentially pathogenic cysteine proteases such as Streprococcus pyogenes SpeB and Porphyromonas gingivalis periodontain.

  16. un estudio comparativo

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    Federico Varona

    2007-01-01

    Full Text Available La comunicación efectiva es uno de los mayores retos que tienen hoy las organizaciones y empresas tanto a nivel nacional como internacional (global. Este artículo presenta los resultados de la investigación realizada por un equipo internacional de investigadores interesados en descubrir y comparar las conductas comunicativas o estilos de comunicación de los empleados finlandeses y mexicanos cuando interactúan con sus superiores. Para ello presentamos: primero, un breve marco teórico del estudio; segundo, la metodología; tercero, los resultados del análisis estadístico comparativo entre los empleados de Finlandia y México; cuarto, las conclusiones generales y su explicación cultural; y quinto, las implicaciones teóricas y prácticas de este estudio con respecto a las competencias comunicativas necesarias para la comunicación efectiva entre empleados y superiores tanto en organizaciones nacionales como internacionales (globales.

  17. Factores de riesgo de las diarreas complicadas en niños que ingresan en terapia intensiva

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    Mariela González Acosta

    2014-08-01

    Full Text Available Se realizó un estudio analítico, de tipo caso de control, en los pacientes ingresados en el hospital provincial pediátrico “Mártires de Las Tunas” con diagnóstico de enfermedad diarreica aguda complicada. Se estudió el período comprendido entre el primero de enero y el 31 de diciembre de 2012, para determinar los factores de riesgo que incidieron en la complicación de esta enfermedad. El universo estuvo constituido por todos los pacientes con diagnóstico de enfermedad diarreica aguda. La muestra se dividió en dos grupos: uno con 70 niños, que ingresaron en la sala de terapia intensiva del referido hospital en este periodo (grupo estudio con diagnóstico de enfermedad diarreica aguda complicada; y otro, con 140, que incluyó a aquellos pacientes que ingresaron en la sala de gastroenterología y no presentaron complicaciones (grupo control. Los datos se recogieron a partir de fuentes primarias y se almacenaron en una base de datos, creada con el programa computarizado Epiinfo versión 6; se realizó el procesamiento estadístico con cálculo de Odds ratio, con índice de confiabilidad de 95%. Se encontró que en las diarreas complicadas existió asociación con los siguientes factores: edad menor de un año, desnutrición, inmunodepresión, malas condiciones socioeconómicas, bajo nivel cultural en los padres, enfermedades crónicas asociadas y el uso previo de medicamentos

  18. Biogeographical aspects of the occurrence of Nyssomyia neivai and Nyssomyia intermedia (Diptera: Psychodidae in a sympatric area of the Brazilian savannah

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    Lara Saraiva

    2012-11-01

    Full Text Available Nyssomyia intermedia and Nyssomyia neivai constitute a species complex associated with Leishmania transmission. The aim of this study was to analyse the ecological profiles of the Ny. intermedia and Ny. neivai populations in a sympatric area in the Brazilian savannah along the banks of the Velhas River. Captures were performed from July 2003-June 2005 in two distinct environments: a gallery forest with various degrees of anthropogenic modification and animal shelters. A total of 20,508 Ny. neivai (86% and Ny. intermedia (14% sandflies were collected. The difference between the proportions of the sandflies that were collected (Ny. neivai/Ny. intermedia per bank was significant. The right bank presented a greater number of sandflies (65% and more preserved vegetation. The abundance of Ny. neivai was higher than that of Ny. intermedia on both banks. The results demonstrate that anthropic activities can affect the sandfly populations in this area, thereby leading to a reduction in species abundance. Nevertheless, the environments with higher levels of antropogenic modification displayed sandfly population numbers that favour the Leishmania transmission cycle.

  19. Acquisition of complement inhibitor serine protease factor I and its cofactors C4b-binding protein and factor H by Prevotella intermedia.

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    Sven Malm

    Full Text Available Infection with the Gram-negative pathogen Prevotella intermedia gives rise to periodontitis and a growing number of studies implies an association of P. intermedia with rheumatoid arthritis. The serine protease Factor I (FI is the central inhibitor of complement degrading complement components C3b and C4b in the presence of cofactors such as C4b-binding protein (C4BP and Factor H (FH. Yet, the significance of complement inhibitor acquisition in P. intermedia infection and FI binding by Gram-negative pathogens has not been addressed. Here we show that P. intermedia isolates bound purified FI as well as FI directly from heat-inactivated human serum. FI bound to bacteria retained its serine protease activity as shown in degradation experiments with (125I-labeled C4b. Since FI requires cofactors for its activity we also investigated the binding of purified cofactors C4BP and FH and found acquisition of both proteins, which retained their activity in FI mediated degradation of C3b and C4b. We propose that FI binding by P. intermedia represents a new mechanism contributing to complement evasion by a Gram-negative bacterial pathogen associated with chronic diseases.

  20. Acquisition of complement inhibitor serine protease factor I and its cofactors C4b-binding protein and factor H by Prevotella intermedia.

    Science.gov (United States)

    Malm, Sven; Jusko, Monika; Eick, Sigrun; Potempa, Jan; Riesbeck, Kristian; Blom, Anna M

    2012-01-01

    Infection with the Gram-negative pathogen Prevotella intermedia gives rise to periodontitis and a growing number of studies implies an association of P. intermedia with rheumatoid arthritis. The serine protease Factor I (FI) is the central inhibitor of complement degrading complement components C3b and C4b in the presence of cofactors such as C4b-binding protein (C4BP) and Factor H (FH). Yet, the significance of complement inhibitor acquisition in P. intermedia infection and FI binding by Gram-negative pathogens has not been addressed. Here we show that P. intermedia isolates bound purified FI as well as FI directly from heat-inactivated human serum. FI bound to bacteria retained its serine protease activity as shown in degradation experiments with (125)I-labeled C4b. Since FI requires cofactors for its activity we also investigated the binding of purified cofactors C4BP and FH and found acquisition of both proteins, which retained their activity in FI mediated degradation of C3b and C4b. We propose that FI binding by P. intermedia represents a new mechanism contributing to complement evasion by a Gram-negative bacterial pathogen associated with chronic diseases.

  1. DE AGUA. ESTUDIO PRELIMINAR

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    Esther E. Pellizzari

    2015-01-01

    Full Text Available El objetivo del presente estudio fue investigar la resistencia al arsénico en cultivos puros de Pseudomonas aeruginosa , aislada de aguas subterráneas de Presidencia Roque Sáenz Peña, provincia de Chaco y evaluar la posibilidad de su uso para la remoción de este contaminante presente en las aguas subterráneas. Las cepas fueron inmovilizadas en piedra natural y se cu ltivaron en caldo de sales y 1 mgAs/L . Se observó la resistencia al arsénico y la formación de biofilm , logrando la interacción entre la s células, roca y arsénico . L a remoción de arsénico se evaluó durante 3 meses y el porcentaje de eliminación de arsénico al final del experimento fue 60%.

  2. Segurança na terapia medicamentosa: uma revisão bibliográfica Seguridad en la terapia medicamentosa: una revisión bibliográfica Security in the medicine therapy: a bibliographical revision

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    Anna Bianca Ribeiro Melo

    2008-03-01

    Full Text Available Trata-se de revisão bibliográfica que objetivou analisar as publicações científicas de enfermeiros sobre erros no manejo da terapia medicamentosa realizada pela enfermagem, indexados em bancos de dados no período de 2000 a 2006. Foram pesquisadas as bibliotecas virtuais MedLine, Lilacs e Scielo. A amostra foi constituída por 16 publicações que foram analisadas quanto aos fatores de erro e propostas ou não de barreiras para erro. Destaca-se que estudos em relação a erros em medicação são relativamente recentes e poucos se referem à realidade brasileira. Entre os motivos apontados para a ocorrência de erros tem-se a ilegibilidade da letra do médico na prescrição, a carga de trabalho excessiva da enfermagem, interrupções freqüentes durante as etapas da prescrição e administração de medicamentos, número reduzido de funcionários e inexperiência do profissional de enfermagem que prepara a medicação. Três estudos apresentaram propostas de barreiras relacionadas à informatização do processo.Se trata de una revisión bibliográfica con el objetivo de analizar las publicaciones de enfermeros acerca de errores con la terapia medicamentosa indexados en bancos de datos entre 2000 a 2006. La muestra consistió en 16 estudios que fueron analizados si presentaban los factores del error y si tenían propuestas de barreras para el error. Se ha visto que este tipo de estudio es reciente y que pocos hablan de la realidad brasilera. Entre las causas de error están la letra del médico, la cantidad de trabajo de los enfermeros, inter r upciones frecuentes, número pequeño de profesionales de enfermería y la inexperiencia de quien prepara la medicación. Tres estudios han hablado de barreras a los errores relacionadas a la informatización.It is a bibliographical revision that objectified to analyze scientific publications of nurses on, errors in the handling of the medicine therapy, carried through for the nursing, indexded in

  3. APROXIMACIÓN BIOÉTICA A LAS TERAPIAS REPARATIVAS: TRATAMIENTO PARA EL CAMBIO DE LA ORIENTACIÓN HOMOSEXUAL APROXIMAÇÃO BIOÉTICA ÀS TERAPIAS REPARADORAS: TRATAMENTO PARA A MUDANÇA DE ORIENTAÇÃO HOMOSSEXUAL BIOETHICAL APPROACH TO RE-CONSTRUCTIVE THERAPIES: TREATMENT TO CHANGE THE HOMOSEXUAL ORIENTATION

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    Gabriel Jaime Montoya Montoya

    2006-01-01

    Full Text Available La articulación temática entre bioética y estudios de género resulta evidente, además de vigente, en el análisis de las llamadas terapias reparativas para la "corrección" o cambio de la orientación homosexual. En este artículo se exponen los elementos básicos que, a manera de insumos, permiten el diálogo bioético sobre este asunto. Se exponen los fundamentos de la orientación sexual y su reconocimiento como un derecho humano. Además, se toman algunos elementos teóricos respecto del empleo del poder médico como mecanismo de domesticación. En la parte final se analizan algunas características de las terapias reparativas y se exponen las razones que no hacen éticamente sustentable su aplicaciónA articulação temática entre bioética e estudos de gênero resulta evidente, além de vigente, na análise das chamadas terapias reparadoras de "correção" ou mudança da orientação homossexual. Neste artigo se expõem os elementos básicos que, em forma de insumos, permitem o diálogo bioético sobre este assunto. São apresentados os fundamentos da orientação sexual e seu reconhecimento como um direito humano. Além disso, aborda-se alguns elementos teóricos a respeito do emprego do poder médico como mecnismo de domestificação. Na parte final se analizam algumas características das terapias reparadoras e se expõe as razões que não tornam eticamente sustentável sua aplicaçãoThe interrelation between bioethics and gender studies is clear and contingent. It is obvious in the analysis of the re-constructive therapies for the "correction" or change of the homosexual orientation. This paper shows the fundamental elements which allow the bioethics dialogue about it in a summary way. Also, the fundamentals of the sexual orientation and their acknowledgement as a human right are exposed. Furthermore, some theoretical aspects are taken with respect to the use of physician power in the domesticating process. The paper finalizes

  4. Knowledge of Patients with Mechanical Valve Prostheses Concerning Chronic Oral Anticoagulant Therapy Conocimiento de los pacientes portadores de prótesis valvular mecánica sobre la terapia de anticoagulación oral crónica Conhecimento de pacientes portadores de prótese valvar mecânica sobre a terapia com anticoagulação oral crônica

    Directory of Open Access Journals (Sweden)

    Helena Teixeira Rocha

    2010-08-01

    Full Text Available Adherence to chronic Oral Anticoagulant Therapy (OAT is directly related to patients' understanding of the therapy. This study verified the knowledge of patients with mechanical valve prostheses concerning OAT. This is a contemporary cross-sectional study, the sample is composed of patients with mechanical valve prostheses (MVP in outpatient follow-up. A 10-question instrument was used; answers were ‘know' (1 point, ‘know partially' (half point, or ‘do not know' (zero. Patients were grouped according to the result obtained: ≤ 4 points was considered insufficient knowledge; > 4 ≤ 8 equated to moderate knowledge; and > 8 was considered appropriate knowledge. Of the 110 patients, 61.8% presented moderate knowledge, 40.9% were not able to name at least one factor that may alter the INR (International Normalized Ratio and 37.3% were not able to report their INR target range. The majority of patients presented moderate knowledge concerning the treatment. Strategies to improve knowledge on the topic should be implemented to minimize risks.El objetivo de este estudio fue verificar el conocimiento de pacientes con prótesis valvular sobre la terapia de anticoagulación oral. La adhesión a la terapia de anticoagulación oral crónica está directamente relacionada al entendimiento del paciente sobre esta terapia. Se trata de un estudio transversal contemporáneo, en el cual se incluyeron pacientes de ambulatorio con prótesis valvular mecánica (PVM. Se aplicó un cuestionario con 10 preguntas; las respuestas fueron "sabe" (1 punto, "sabe parcialmente" (medio punto, "no sabe" (cero puntos. Los pacientes fueron agrupados según la puntuación obtenida: ≤ 4 puntos, conocimiento insuficiente; > 4 ≤ 8 puntos, conocimiento regular; > 8 puntos, conocimiento adecuado. De los 110 pacientes incluidos, el 61,8% demostró conocimiento regular, 40,9% no supieron citar un factor que altera el REI (razón estandarizada internacional y 37,3% no supieron

  5. Paciente quirúrgico séptico en terapia intensiva

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    Rubén Bembibre Taboada

    1997-08-01

    Full Text Available Se realizó un estudio anual de 121 pacientes quirúrgicos sépticos ingresados en Terapia Intensiva en el Hospital Provincial Clinicoquirúrgico Docente "Dr. Gustavo Aldereguía Lima" y se aplicaron métodos computadorizados para el procesamiento de datos y aplicación de pruebas de significación estadística. La edad media de la serie fue de 40,4 para vivos y 47,35 para fallecidos; la mayor letalidad correspondió a pancreatitis hemorrágica (80 y cirugía gastroduodenal (45,5. En el 41,32 % el diagnóstico se basó en el examen físico y ultrasonido abdominal. Existió un incremento franco de evolución desfavorable al aumentar el número de intervenciones quirúrgicas. Los gérmenes más frecuentes fueron Klebsiella pneumoniae, E. Coli, Pseudomona aureginosa y Proteus mirabilis. Los anti-bióticos más usados fueron metronidazol, penicilinas, y aminoglucósidos. Se destaca el 8,2 de incidencia para fallo multiórgano (FMO con 100 % de letalidad y un predominio de la evolución favorable en 84 enfermos y 37 que fallecieronA one year-period study of 121 surgical septic patients admitted to the Intensive Care Unit of "Dr. Gustavo Aldereguía Lima" Clinicosurgical Provincial Hospital was carried out by the application of computerized methods for data processing and the application of statistical significance tests. Average age of the series was 40.4 for alive subjects, and 47.35 for deceased subjects; the greatest lethality rate corresponded to haemorrhagic pancreatitis (80 and to gastroduodenal surgery (45.5. In 41.32 % the diagnosis was based on physical examination and abdominal ultrasound. There was a frank increase of unfavourable evolution as the number of surgical procedures increased. Germs most frequently found were Klebsiella pneumonia, E. coli, Pseudomona aeruginosa and Proteus mirabilis. Antibiotics most frequently used were metronidazole, penicillins, and aminoglycosides. An incidence of 8.2 for multiple organ failure is highlithed

  6. Clinical evolution of adult, elderly and very elderly patients admitted in Intensive Care Units Evolución clínica de adultos, ancianos y muy ancianos internados en Unidades de Terapia Intensiva Evolução clínica de adultos, idosos e muito idosos internados em Unidade de Terapia Intensiva

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    Verônica Cunha Rodrigues de Oliveira

    2011-12-01

    Full Text Available This study compared clinical outcomes among adult, elderly and very elderly patients admitted to Intensive Care Units (ICUs located in São Paulo, Brazil. This retrospective, longitudinal and comparative study included 279 adult (≥18 and El estudio comparó la evolución clínica de adultos, ancianos y muy ancianos internados en Unidades de Terapia Intensiva localizadas en Sao Paulo, Brasil. El estudio fue retrospectivo longitudinal, del tipo comparativo. Participaron 279 adultos (≥18 y O estudo comparou a evolução clínica de adultos, idosos e muito idosos, internados em Unidades de Terapia Intensiva, localizadas em São Paulo, Brasil. Trata-se de estudo retrospectivo-longitudinal, do tipo comparativo. Participaram 279 adultos (≥18 e <60 anos, 216 idosos (≥60 e <80 anos e 105 muito idosos (≥80 anos. Os adultos diferiram dos outros grupos em relação à unidade de destino e evolução da gravidade, segundo Simplified Acute Physiology Score II. Foi mais prevalente o encaminhamento dos adultos para unidades de internação, porém, os idosos e muito idosos, sobreviventes à internação na unidade crítica, apresentaram melhora mais acentuada antes da alta. Entre adultos e idosos ocorreu diferença em relação à mortalidade, com maior taxa no grupo mais velho; entretanto, a mortalidade dos muito idosos e adultos foi similar. Em geral, os resultados indicaram que a idade mais avançada não foi fator associado aos desfechos indesejáveis da assistência intensiva.

  7. AS CORES DO AMBIENTE DA UNIDADE DE TERAPIA INTENSIVA

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    Nélio Barbosa Boccanera

    2004-12-01

    Full Text Available Os objetivos deste trabalho foram descrever as cores mais freqüentemente utilizadas nas paredes, tetos, mobiliários e roupas das Unidades de Terapia Intensiva e verificar com os funcionários e pacientes desta unidade as cores que gostariam que existissem dentro deste ambiente e a associação que eles estabelecem com as cores. Foi realizada pesquisa descritiva exploratória, com enfoque quanti-qualitativo. A amostra foi constituída de pacientes e profissionais da UTI de três hospitais públicos de Goiânia em 2002. Observou-se a existência de cores monótonas e neutras nestes ambientes, em tons que vão do branco ao preto, enquanto outras nuanças de cores aparecem num percentual menor. Constatou-se uma preferência pelas cores azul-claro, branco, verde-claro e amarelo entre os dois seguimentos pesquisados. Concluiu-se que as pessoas estabelecem associações com as cores; que a cor pode auxiliar no estabelecimento do equilíbrio e contribuir para harmonia do corpo, da mente e das emoções.

  8. Cultura paulista na terapia comunitária integrativa

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    Josefa Emília Lopes Ruiz

    2016-12-01

    Full Text Available Este trabalho propõe resgatar os elementos típicos da cultura do estado de São Paulo e sua influência na constituição dos indivíduos enquanto valores, crenças e práticas que devem ser trabalhados no módulo de Antropologia Cultural do curso de Terapia Comunitária Integrativa, uma prática em saúde mental criada pelo Prof. Dr. Adalberto Barreto, que prioriza o acolhimento e a inclusão social.  Consta o histórico do surgimento da TCI em vários polos formadores do estado e como o tema cultura vem sendo trabalhado nos cursos de formação. A ênfase na formação está no reconhecimento e respeito à diversidade e na valorização da cultura pelo indivíduo, funcionando como um agente de "empoderamento", melhorando sua autoestima e colaborando na transformação da realidade.

  9. El abandono en las terapias psicológicas

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    Manuel Porcel Medina

    2005-01-01

    Full Text Available En el siguiente trabajo teórico queremos mostrar algunas reflexiones en torno al fenómeno conocido como abandono en las terapias psicológicas, haciendo hincapié en su naturaleza, en su uso como parámetro predictor de éxitos terapéuticos y en la conveniencia o no de intentar reducir o aminorar dicho fenómeno. A través de la incursión teórica en la comprensión del abandono, concluiremos que: las ceremonias de tratamiento médico-biológico, esto es, el proceso terapéutico que sigue el curso: síntomas-diagnóstico-tratamiento, tiene una serie de características que lo hacen sumamente facilitador del fenómeno de abandono psicoterapéutico. Como veremos, esta facilitación vendrá mediada porque este tipo de ceremonias pueden propiciar el fenómeno que llamamos conflicto de normas irresuelto personalmente, a través de la adquisición, por parte del paciente de “un papel pasivo en su curación”.

  10. Hydroxyurea appears beneficial in patients with beta-thalassaemia major and intermedia

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    S. F.S.A. Abdul Wahid

    2007-06-01

    Full Text Available Patients with severe inherited β-globin chain disorders may have milder illness if they produce high levels of fetal hemoglobin (HbF. Hydroxyurea (HU has been shown to enhance HbF levels in patients with sickle cell disease and may be useful in β-thalassemias. We administered HU to 13 patients with β-thalassemia intermedia or major, including 6 splenectomized patients. The patients received escalating doses (10 to 25 mg/kg/d of HU for around 2 years (median: 21 months, range: 8 - 55 months. Eleven patients responded with an increase in the pre-transfusion HbF levels, from a base line median of 8.0% (2.5 - 61.3% to 28.0% (6.6 - 49.2% and 40.7% (4.8 - 72.3% at 3 months and 18 months post-HU, respectively. A concomitant increment in median hemoglobin levels was noted at 1, 3 and 18 months of HU therapy. Six of 7 transfusion-dependent patients who had an increment of HbF (one with β-thalassemia major also had reduced transfusion requirement over the 2-year period of HU therapy. Response to HU was also shown by a reduction in spleen size. Apart from oral ulcers that resolved upon dose reduction of HU, no significant toxicity was noted. We conclude that increased HbF production in β-thalassemia patients, with an improvement in erythropoiesis, can be achieved using HU with minimal toxicity. (Med J Indones 2007; 16:78-83 Keywords: fetal hemoglobin (HbF, erythropoiesis, toxicity

  11. Plasma fructosamine concentrations in horses with pituitary pars intermedia dysfunction with and without laminitis.

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    Knowles, E J; Menzies-Gow, N J; Mair, T S

    2014-03-01

    Plasma fructosamine concentration ([fructosamine]) is believed to reflect medium term, average blood glucose concentration and in a previous study was higher in horses with active laminitis than in normal horses. Pituitary pars intermedia dysfunction (PPID) is associated with hyperglycaemia and laminitis. To test the hypotheses that: [fructosamine] is higher in PPID cases than normal animals; furthermore, that within cases of PPID [fructosamine] is higher in those with active laminitis than nonlaminitic cases and in cases that have been affected by active laminitis in the preceding year than those that have not. Observational, case-control/cross-sectional study. [Fructosamine] was measured in cases of PPID (n = 46) and normal animals (n = 139). A normal range was calculated; values were compared between the 2 groups and within the PPID group, between cases with and without active laminitis and between cases that had and had not been affected by active laminitis in the preceding year. In normal animals mean [fructosamine] was 248.7 μmol/l; the normal range (mean ± 2 s.d.) was 195.5-301.9 μmol/l. Plasma [fructosamine] was not higher in PPID cases than in normal animals. In PPID cases, [fructosamine] was significantly (P = 0.006) higher in cases with active laminitis (mean ± s.d. 261.2 ± 39.2 μmol/l) compared with those without active laminitis (234.5 ± 32.9 μmol/), but [fructosamine] was not higher in cases that had been affected by active laminitis in the preceding year than those that had not. In horses affected by PPID, [fructosamine] is higher in cases with active laminitis than in cases without active laminitis. Clinical use of [fructosamine] is limited due to overlap with the normal range. Further studies into glucose and protein metabolism in cases of PPID are warranted. © 2013 EVJ Ltd.

  12. Molecular Basis of β-Thalassemia Intermedia in Erbil Province of Iraqi Kurdistan.

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    Shamoon, Rawand P; Al-Allawi, Nasir A S; Cappellini, Maria D; Di Pierro, Elena; Brancaleoni, Valentina; Granata, Francesca

    2015-01-01

    β-Thalassemia intermedia (β-TI) is a clinical term describing a range of clinical phenotypes that are intermediate in severity between the carrier state and β-thalassemia major (β-TM). To characterize the molecular basis of β-TI in Erbil Province, Northern Iraq, 83 unrelated patients were investigated. Detection of β-globin gene mutations was carried out by reverse hybridization assay and direct gene sequencing. All patients were screened for the XmnI polymorphism by direct sequencing of HBG2 ((G)γ promoter gene). Detection of α-globin gene deletions and triplication was carried out using the reverse hybridization assay. Four main molecular patterns were identified in association with the β-TI phenotype, namely: β(+)/β(+) (38.5%), β(+)/β(0) (21.6%), β(0)/β(0) (31.3%), and β(0)/wild type (8.4%). IVS-I-6 (T > C) was the most frequently encountered mutation (55 alleles, 34.6%), followed by IVS-II-1 (G > A) and codon 8 (-AA); furthermore, we report for the first time from Iraq two β(+) mutations, -87 (C > G) and 5' untranslated region (5'UTR) +22 (G > A). The XmnI polymorphism was detected in 47.0% of patients, mainly in association with the β(0)/β(0) genotype. The α-globin gene deletions were encountered in four cases, including one case with (- -(FIL)) double gene deletion, a report that is the first from our country. The α-globin gene triplication was detected in five of the seven heterozygous β-thalassemia (β-thal) patients. Similar to other Mediterranean countries, inheritance of mild β-globin mutations was the main molecular pattern underlying β-TI in our patients followed by the ameliorating effect of the XmnI polymorphism.

  13. Byrsonima intermedia A. Juss.: gastric and duodenal anti-ulcer, antimicrobial and antidiarrheal effects in experimental rodent models.

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    Santos, Raquel Cássia; Kushima, Hélio; Rodrigues, Clenilson Martins; Sannomiya, Miriam; Rocha, Lúcia Regina Machado; Bauab, Taís Maria; Tamashiro, Jorge; Vilegas, Wagner; Hiruma-Lima, Clélia Akiko

    2012-03-27

    An ethnopharmacological survey indicated that the leaves of Byrsonima intermedia A. Juss. (Malpighiaceae), a medicinal species commonly found in the Brazilian Cerrado, can be used against gastroduodenal disorders, such as gastric ulcers and diarrhea. The objective of this study was to evaluate the effects of a methanolic extract of Byrsonima intermedia (MBI) leaves on gastric and duodenal ulcers and to assess the antimicrobial and antidiarrheal effects of this extract. The anti-ulcerogenic effect of MBI was investigated with different ulcerogenic agents in rodents (mice and rats), including non-steroidal anti-inflammatory drug (NSAID), HCl/ethanol, pyloric ligature, absolute ethanol, cysteamine and ischemia-reperfusion. The gastroprotective effect of MBI was assessed by analysing the volume of gastric juice, pH, total acidity, mucus, NO, sulfhydryl compound, vanilloid receptor, glutathione (GSH) levels, and myeloperoxidase (MPO) activity in the gastric and duodenal mucosa. The gastric and duodenal healing effects of MBI were also evaluated during 7 or 14 days of treatment. The antidiarrheal action (measured by intestinal motility and diarrhea induced by castor oil) and anti-bacterial action of MBI against Staphylococcus aureus, Escherichia coli and Helicobacter pylori were also evaluated by microdilution methods. The phytochemical profile from MBI indicated the presence of phenolic acids, flavan-3-ols, oligomeric proanthocyanidins, and flavonoids. MBI (500mg/kg, p.o.) significantly inhibited totally gastric and duodenal lesions (69%) and healed gastric (49% on 14 days) and duodenal lesions (45% on 7 and 14 days). The MBI exert gastroprotective action by participation of endogenous sulfhydryl compounds, vanilloid receptors and increase in GSH level to effective gastric and duodenal protection. MBI also displayed curative (42%) and preventive (49%) antidiarrheal effects by involvement of opiate receptors and also antimicrobial effects in vitro. Byrsonima intermedia

  14. Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens in endodontic lesions detected by culture and by PCR.

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    Gomes, B P F A; Jacinto, R C; Pinheiro, E T; Sousa, E L R; Zaia, A A; Ferraz, C C R; Souza-Filho, F J

    2005-08-01

    he aim of this study was to investigate the presence of four black-pigmented bacteria, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens, in endodontic infections by culture and polymerase chain reaction (PCR) analyses. Microbial samples were obtained from 50 teeth with untreated necrotic pulps (primary infection) and from 50 teeth with failing endodontic treatment (secondary infection). Microbiological strict anaerobic techniques were used for serial dilution, plating, incubation, and identification. For PCR detection, the samples were analyzed using species-specific primers of 16S rDNA and the downstream intergenic spacer region. Culture and PCR detected the test species in 13/100 and 50/100 of the study teeth, respectively. The organisms were cultured from 11/50 (22%) of primarily infected root canal samples and from 2/50 (4%) of secondary root canal samples. PCR detection identified the target species in 32/50 (64%) and 18/50 (36%) of primary and secondary infections, respectively. P. gingivalis was rarely isolated by culture methods (1%), but was the most frequently identified test species by PCR (38%). Similarly, P. endodontalis was not recovered by culture from any tooth studied, but was detected by PCR in 25% of the sampled teeth. PCR-based identification also showed higher detection rates of P. intermedia (33%) and P. nigrescens (22%) than culture (13%). In conclusion, P. gingivalis, P. endodontalis, P. intermedia, and P. nigrescens were identified more frequently in teeth with necrotic pulp than in teeth with failing endodontic treatment. Also, a higher frequency of black-pigmented species was detected by PCR than by culture.

  15. La terapia breve: una alternativa de intervención psicológica efectiva

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    Nohelia Hewitt Ramírez

    2009-10-01

    Full Text Available El objetivo de este trabajo es describir y estructurar los orígenes, bases teóricas y los procedimientos de la terapia breve desde la perspectiva terapéutica cognitivo conductual. Se inicia con la identificación de las bases teóricas de la terapia breve, principalmente las teorías motivacionales relacionadas con la disponibilidad al cambio y la autoeficacia desde la teoría social cognitiva. Se realiza un paralelo entre las características principales de la terapia breve en contraposición con las terapias tradicionales. Por último, se propone una estructura procedimental general de estrategias clínicas a emplear en la terapia breve con soporte empírico, la cual puede ser utilizada en diferentes problemáticas con variaciones específicas de acuerdo con el problema tratado o las características específicas del consultante.

  16. La terapia breve: una alternativa de intervención psicológica efectiva

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    Carlos Andrés Gantiva Díaz

    2009-06-01

    Full Text Available El objetivo de este trabajo es describir y estructurar los orígenes, bases teóricas y los procedimientos de la terapia breve desde la perspectiva terapéutica cognitivo conductual. Se inicia con la identifi cación de las bases teóricas de la terapia breve, principalmente las teorías motivacionales relacionadas con la disponibilidad al cambio y la autoefi cacia desde la teoría social cognitiva. Se realiza un paralelo entre las características principales de la terapia breve en contraposición con las terapias tradicionales. Por último, se propone una estructura procedimental general de estrategias clínicas a emplear en la terapia breve con soporte empírico, la cual puede ser utilizada en diferentes problemáticas con variaciones específi cas de acuerdo con el problema tratado o las características específi cas del consultante

  17. Biology of the first generation of a laboratory colony of Nyssomyia intermedia (Lutz & Neiva, 1912 and Nyssomyia neivai (Pinto, 1926 (Diptera: Psychodidae

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    José Dilermando Andrade Filho

    2004-10-01

    Full Text Available The phlebotomine sand flies Nyssomyia intermedia (Lutz & Neiva, 1912 and Nyssomyia neivai (Pinto, 1926 are very close and may be involved in the transmission of Leishmania spp. Ross, 1903 in Brazil. The biology of the first laboratory-reared generations of these species, descended from insects captured in Além Paraíba (N. intermedia and Corinto (N. neivai in the Brazilian state of Minas Gerais, is described here. The captured females were fed on hamsters and maintained individually in rearing pots. Laboratory temperature and relative humidity were maintained at 25-26ºC and 80% respectively. The productivity of the first generation of N. intermedia was greater than that of N. neivai, and its development time clearly shorter, particularly for the second and third larval instars.

  18. Biology of the first generation of a laboratory colony of Nyssomyia intermedia (Lutz & Neiva, 1912) and Nyssomyia neivai (Pinto, 1926) (Diptera: Psychodidae).

    Science.gov (United States)

    Andrade Filho, José Dilermando; Galati, Eunice A Bianchi; Falcão, Alda Lima

    2004-10-01

    The phlebotomine sand flies Nyssomyia intermedia (Lutz & Neiva, 1912) and Nyssomyia neivai (Pinto, 1926) are very close and may be involved in the transmission of Leishmania spp. Ross, 1903 in Brazil. The biology of the first laboratory-reared generations of these species, descended from insects captured in Além Paraíba (N. intermedia) and Corinto (N. neivai) in the Brazilian state of Minas Gerais, is described here. The captured females were fed on hamsters and maintained individually in rearing pots. Laboratory temperature and relative humidity were maintained at 25-26 masculineC and 80% respectively. The productivity of the first generation of N. intermedia was greater than that of N. neivai, and its development time clearly shorter, particularly for the second and third larval instars.

  19. Eficacia y seguridad de la terapia tópica con capsaicina 0,075% versus mentol 1%, en el tratamiento del prurito de la foliculitis eosinofílica asociada al virus de la inmunodeficiencia adquirida

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    Carlos Galarza

    2007-09-01

    Full Text Available Objetivo: Determinar la eficacia y seguridad de la terapia tópica con capsaicina 0,075% versus mentol 1% en el tratamiento del prurito de la foliculitis eosinofílica asociada al virus de la inmunodeficiencia adquirida (VIH. Diseño: Estudio clínico aleatorizado y doble ciego. Lugar: Servicios de Enfermedades Infecciosas y Tropicales (SEIT y Dermatología, Hospital Nacional Dos de Mayo, Lima, Perú. Participantes: Pacientes con foliculitis eosinofílica asociada al VIH. Intervenciones: La muestra estuvo constituida por 40 pacientes con foliculitis eosinofílica asociada al VIH, quienes fueron distribuidos mediante muestreo aleatorio sistemático en dos grupos: el grupo 1 conformado por 20 pacientes que recibieron la terapia 1; y el grupo 2, conformado por 20 pacientes que recibieron la terapia 2. Los pacientes recibieron tratamiento con capsaicina al 0,075% y mentol 1%; la aplicación se realizó en el área afectada, cada 6 horas, durante 45 días. Se implementó un sistema de visitas (domiciliarias u hospitalarias, se evaluó la eficacia en la reducción del prurito (escala de Likert y se registró los eventos. Principales medidas de resultados: Respuesta clínica a la administración de capsaicina y mentol. Resultados: La respuesta fue buena en 90% de los pacientes que recibieron capsaicina 0,075%, en comparación con 40% en el grupo que recibió mentol al 1% (p = 0,001, con OR = 0,074 (IC 95 %: 0,013 - 0,411. La eficacia de las terapias tópicas fue modificada por el recuento de linfocitos CD4 (p 0,05. Conclusiones: La terapia tópica con capsaicina es eficaz y segura en el tratamiento del prurito, en la foliculitis eosinofílica asociada al VIH.

  20. α:Non–α and Gγ:Aγ globin chain ratios in thalassemia intermedia patients treated with hydroxyurea

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    Abbas Najjari

    2014-05-01

    Conclusions: Improvement in α:non-α ratio and consequent decrease of free α-globin chain might be the cause of beneficial effects of hydroxyurea therapy. Two patients who felt better didn't show significant increase in their fetal hemoglobin level, and this is in contradiction with the hypothesis claiming that the HbF level increase is the cause of such therapeutic effect. In spite of the unclear mechanism of action of this drug, hydroxyurea therapy had noticeable impacts on thalassemia intermedia and also sickle cell disease and even patients suffering from thalassemia major.

  1. Anti-inflammatory effects and the underlying mechanisms of action of daidzein in murine macrophages stimulated with Prevotella intermedia lipopolysaccharide.

    Science.gov (United States)

    Choi, E-Y; Jin, J-Y; Lee, J-Y; Choi, J-I; Choi, I S; Kim, S-J

    2012-04-01

    Host modulatory agents directed at inhibiting specific proinflammatory mediators could be beneficial in terms of attenuating periodontal disease progression and potentially enhancing therapeutic responses. The aim of this study was to investigate whether daidzein could modulate the production inflammatory mediators in macrophages stimulated with lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen implicated in periodontal disease, and to delineate underlying mechanisms of action. LPS was extracted from P. intermedia ATCC 25611 cells by the standard hot phenol-water method. The amounts of nitric oxide (NO) and interleukin-6 (IL-6) secreted into the culture medium were assayed. A real-time PCR was performed to quantify inducible nitric oxide synthase (iNOS) and IL-6 mRNA expression. We used immunoblot analysis to characterize iNOS protein expression, phosphrylation of c-Jun N-terminal kinase (JNK) and p38, degradation of inhibitory κB-α (IκB-α), nuclear translocation of nuclear factor-κB (NF-κB) subunits and phosphorylation of signal transducer and activator of transcription 1 (STAT1). The DNA-binding activity of NF-κB was assessed by using ELISA-based kits. Daidzein significantly inhibited the production of NO and IL-6, as well as their mRNA expression, in P. intermedia LPS-treated RAW264.7 cells. The JNK and p38 pathways were not involved in the regulation of LPS-induced NO and IL-6 release by daidzein. Daidzein inhibited the degradation of IκB-α induced by P. intermedia LPS. In addition, daidzein suppressed NF-κB transcriptional activity via regulation of the nuclear translocation and DNA-binding activity of NF-κB p50 subunit and blocked STAT1 phosphorylation. Although additional studies are required to dissect the molecular mechanism of action, our results suggest that daidzein could be a promising agent for treating inflammatory periodontal disease. Further research in animal models of periodontitis is necessary to better evaluate the

  2. β-Lactamase Production in Prevotella intermedia, Prevotella nigrescens, and Prevotella pallens Genotypes and In Vitro Susceptibilities to Selected Antimicrobial Agents

    Science.gov (United States)

    Mättö, Jaana; Asikainen, Sirkka; Väisänen, Marja-Liisa; Von Troil-Lindén, Birgitta; Könönen, Eija; Saarela, Maria; Salminen, Kari; Finegold, Sydney M.; Jousimies-Somer, Hannele

    1999-01-01

    The present study investigated the β-lactamase production of 73 Prevotella intermedia, 84 Prevotella nigrescens, and 14 Prevotella pallens isolates and their in vitro susceptibilities to six antimicrobial agents. The P. intermedia and P. nigrescens isolates were recovered from oral and extraoral samples obtained from subjects in two geographic locations from 1985 to 1995. The clonality of the β-lactamase-positive and β-lactamase-negative isolates and the clustering of the genotypes were studied by arbitrarily primed-PCR fingerprinting. β-Lactamase production was detected in 29% of P. intermedia isolates, 29% of P. nigrescens isolates, and 57% of P. pallens isolates. No difference in the frequencies of β-lactamase production by P. intermedia and P. nigrescens between isolates from oral and extraoral sites, between isolates obtained at different time periods, or between P. intermedia isolates from different geographic locations was observed. However, the P. nigrescens isolates from the United States were significantly more frequently (P = 0.015) β-lactamase positive than those from Finland. No association between the genotypes and β-lactamase production or between the genotypes and the sources of the isolates was found. The penicillin G MICs at which 90% of the isolates were inhibited were 8 μg/ml for P. intermedia, 8 μg/ml for P. nigrescens, and 16 μg/ml for P. pallens. For the β-lactamase-negative isolates, the corresponding values were 0.031, 0.031, and 0.125 μg/ml, and for the β-lactamase-positive isolates, the corresponding values were 16, 8, and 32 μg/ml. All isolates were susceptible to amoxicillin-clavulanate, cefoxitin, metronidazole, azithromycin, and trovafloxacin. The MICs of amoxicillin-clavulanate and cefoxitin were relatively higher for the β-lactamase-positive population than for the β-lactamase-negative population. PMID:10508011

  3. Prevotella intermedia lipopolysaccharide stimulates release of tumor necrosis factor-alpha through mitogen-activated protein kinase signaling pathways in monocyte-derived macrophages.

    Science.gov (United States)

    Kim, Sung-Jo; Choi, Eun-Young; Kim, Eun Gyung; Shin, Su-Hwa; Lee, Ju-Youn; Choi, Jeom-Il; Choi, In-Soon

    2007-11-01

    The purpose of this study was to investigate the effects of lipopolysaccharide from Prevotella intermedia, a major cause of inflammatory periodontal disease, on the production of tumor necrosis factor (TNF)-alpha and the expression of TNF-alpha mRNA in differentiated THP-1 cells, a human monocytic cell line. The potential involvement of the three main mitogen-activated protein kinase (MAPK) signaling pathways in the induction of TNF-alpha production was also investigated. Lipopolysaccharide from P. intermedia ATCC 25611 was prepared by the standard hot phenol-water method. THP-1 cells were incubated in the medium supplemented with phorbol myristate acetate to induce differentiation into macrophage-like cells. It was found that P. intermedia lipopolysaccharide can induce TNF-alpha mRNA expression and stimulate the release of TNF-alpha in differentiated THP-1 cells without additional stimuli. Treatment of the cells with P. intermedia lipopolysaccharide resulted in a simultaneous activation of three MAPKs [extracellular signal-related kinase 1/2 (ERK1/2), c-Jun N-terminal kinase 1/2 (JNK1/2) and p38]. Pretreatment of the cells with MAPK inhibitors effectively suppressed P. intermedia lipopolysaccharide-induced TNF-alpha production without affecting the expression of TNF-alpha mRNA. These data thus provided good evidence that the MAPK signaling pathways are required for the regulation of P. intermedia lipopolysaccharide-induced TNF-alpha synthesis at the level of translation more than at the transcriptional level.

  4. Efectividad de distintas terapias físicas en el tratamiento conservador de la fascitis plantar. Revisión sistemática

    Directory of Open Access Journals (Sweden)

    Ana María Díaz López

    2014-01-01

    Full Text Available Fundamentos: La fascitis plantar es la afección más frecuente de dolor no traumático en el tobillo-pie. Se presenta más en mujeres entre los 40-70 años con comienzo progresivo y difuso en planta del pie o tobillo que poco a poco se agudiza impidiendo la marcha. El objetivo de este trabajo es determinar si las distintas terapias físicas utilizadas en el tratamiento conservador de la fascitis plantar de al menos un mes de evolución son efectivas individualmente y/o combinadas entre sí. Métodos: Revisión sistemática en las bases de datos The Cochrane Library Plus, Medline, Lilacs, IBECS, IME, PEDro y Enfispo sin restricción de fecha, en español e inglés. Se incluyeron ensayos clínicos controlados aleatoriamente con personas adultas diagnosticadas de fascitis plantar, estudios de intervención, prospectivos y revisiones sistemáticas. La evaluación de la elegibilidad de los estudios fue desarrollada por dos revisores de manera independiente, estandarizada y no cegada. Para clasificarlos, se utilizó la escala crítica PEDro, formulario de calidad metodológica y una revisión crítica de cada resumen y si esto no fue concluyente evaluación del texto completo. Resultados: Se revisó un total de 32 artículos a texto completo. Las técnicas más empleadas fueron los estiramientos y las ondas de choque, aunque los mejores resultados se obtuvieron combinando varias técnicas. Las ondas de choque fueron efectivas cuando otras técnicas fracasaron. Conclusiones: Las terapias físicas empleadas en los distintos estudios han demostrado ser eficaces aunque en distinta medida ya sea para disminuir el dolor o aliviar los síntomas de la fascitis plantar.

  5. Avaliação da adesão à terapia antirretroviral em pacientes com AIDS Evaluation of adherence to antiretroviral therapy for AIDS patients

    Directory of Open Access Journals (Sweden)

    Richardson Augusto Rosendo da Silva

    2017-01-01

    RESUMEN: Objetivo: Este estudio tuvo como objetivo evaluar la adherencia al tratamiento antirretroviral para pacientes con SIDA.Método: Estudio de carácter cuantitativo, campo, llevado a cabo en un centro de referencia para las enfermedades infecciosas en Natal / RN, de agosto 2010 a julio 2011. Se recogieron los datos a través de gráficos, cuestionarios y registros de farmacia dispensadora.Resultados: De los 402 pacientes estudiados (70,2% eran varones, con una media de 35 años, y el 90,0% mostraron contaminación momento 1-5 años. Se observó que el 30% de los pacientes se adhirió al tratamiento. Conclusión: La adherencia se encuentra más baja que las recomendadas en la literatura, para aumentar la adherencia al tratamiento antirretroviral es esencial para llevar a cabo las estrategias para aumentar el conocimiento y la participación de los usuarios. Descriptores:Cumplimiento de la Medicación; Terapia Antirretroviral Altamente Activa; Infecciones por VIH.

  6. A experiência vivida pelas famílias de crianças hospitalizadas em uma unidade de terapia intensiva neonatal

    Directory of Open Access Journals (Sweden)

    Maria de Lourdes Centa

    2004-01-01

    Full Text Available El objetivo de este estudio fue identificar la experiencia vivida por los familiares de los niños internados en la Unidad de Terapia Intensiva Neonatal (UTIN de un hospital en Curitiba. Los datos fueron obtenidos a travéz de la entrevista semi-estructurada. La muestra estuvo compuesta por 18 familiares de niños internados en la UTIN durante los meses de junio y julio del 2003. Se trata de un estudio exploratório-descriptivo. Después del análisis y la discusión de datos surgió como categoría central “vivenciando el internamiento del recién nacido en la UTI neonatal”, originando las siguientes categorías: definición de la UTI Neonatal; razones de internación del recién nacido; perspectiva de información; significado de la experiencia vivida; sentimientos generados; reacción de los familiares; evaluación del cuidado; participación de los familiares en el cuidado; evaluación de la asistencia recibida y su envolvimento en el cuidado. En “vivenciando la internación del recién nacido en la UTI Neonatal” por los familiares, concluimos que, no obstante la atención prestada por el equipo multiprofesional, ellos sienten la necesidad de mayores informaciones, orientaciones, consejos y apoyo.

  7. O cuidar de crianças egressas da unidade de terapia intensiva neonatal no espaço domiciliar: instrumentos mediadores das famílias

    Directory of Open Access Journals (Sweden)

    Juliana Medeiros de Moraes Rezende Montenegro

    2004-01-01

    Full Text Available Este estudio presenta los instrumentos mediadores que las familias utilizan en el domicilio para cuidar del niño egresado de la terapia intensiva neonatal, con el objectivo de describir y analizar como fueron interiorizados e incorporados por las familias en el cotidiano del cuidado. Se trata de un estudio cualitativo, desarrollado a través del método creativo sensible a través de la implementación de la dinámica grupal cuerpo-saber. El escenario fue el domicilio de las familias inscritas en el Proyecto de Extensión “La salud del bebé comienza en casa” de la UFRJ, distribuidas por el Municipio de Río de Janeiro-Brasil. Participaron cinco grupos familiares, nueve estudiantes académicas y tres enfermeras. Se concluyó que, al cuidar del cuerpo diminuto y desafiador del niño, al lidiar con la demanda de los cuidados medicamentosos y los relacionados a los hábitos de vida diaria, las familias interiorizaron e incorporaron instrumentos disponibles en el medio hospitalario (gasa, algodón, jeringa, remedios, etc y en el medio domiciliario (bacía, balde,vasos, biberones, etc.

  8. Aplicación de la terapia de interacción padres-hijos en un caso de trastorno negativista desafiante

    OpenAIRE

    Ferro, Rafael; Vives, Carmen; Ascanio, Lourdes

    2010-01-01

    La terapia de interacción padres-hijos (PCIT) es una forma de terapia para tratar problemas de la infancia que integra la terapia de juego tradicional y los métodos clásicos operantes. Ha demostrado ser útil en problemas como: desobediencia, hiperac

  9. Concordia. Un estudio comparativo

    Directory of Open Access Journals (Sweden)

    Graciela Mingo de Bevilacqua

    2006-01-01

    Full Text Available Este estudio comparativo buscó develar las heterogeneidades de la pobreza urbana que enmarcan la vida de las familias pobres de dos aglomerados de la Provincia de Entre Ríos: Paraná y Concordia. Se partió del concepto de pobreza como fenómeno heterogéneo, no sólo por su magnitud e intensidad, sino también por su configuración, condicionada por el entorno urbano y las estructuras económico-sociales. En la última década del siglo XX y en los primeros años del actual siglo XXI, la pobreza se fue acentuando; tuvieron lugar en la Argentina las transformaciones estructurales que, sin duda, profundizaron las desigualdades sociales. Ellas se pueden sintetizar en: reforma del estado, privatizaciones y reforma laboral, procesos recesivos y terciarización de la economía. Las perspectivas de descripción y análisis metodológico se encuadran en el estilo de complementariedad, en pos de una aproximación más profunda a la reproducción de la pobreza y las estrategias de los hogares pobres.

  10. Cross-species amplification of primers developed from Plantago major and P. intermedia in two Hawaiian Plantago species from the section Plantago.

    Science.gov (United States)

    Wolff, K; Houston, K; Dunbar-Co, S

    2009-05-01

    Thirty-nine primers, developed from the sister species Plantago major and P. intermedia, were tested in two Hawaiian Plantago species from the section Plantago. Eight primers were polymorphic, of which three were published earlier, and five are new ones presented here. Amplification and polymorphism levels appeared to be high in these Hawaiian species. These markers will be valuable for further mating system and evolutionary studies in species from the section Plantago that are closely related to P. major and P. intermedia. © 2009 Blackwell Publishing Ltd.

  11. Characteristics of β-lactamases and their genes (blaA and blaB in Yersinia intermedia and Y. frederiksenii

    Directory of Open Access Journals (Sweden)

    Sharma Sachin

    2007-04-01

    Full Text Available Abstract Background The presence of β-lactamases in Y. enterocolitica has been reported to vary with serovars, biovars and geographical origin of the isolates. An understanding of the β-lactamases in other related species is important for an overall perception of antibiotic resistance in yersiniae. The objective of this work was to study the characteristics of β-lactamases and their genes in strains of Y. intermedia and Y. frederiksenii, isolated from clinical and non-clinical sources in India. Results The enzymes, Bla-A (a constitutive class A penicillinase and Bla-B (an inducible class C cephalosporinase were found to be present in all the clinical and non-clinical strains of Y. intermedia and Y. frederiksenii by double disc diffusion method. The results showed differential expression of Bla-A as indicated by presence/absence of synergy whereas expression of Bla-B was quite consistent. The presence of these enzymes was also reflected in the high minimum inhibitory concentrations, MIC50 (126–1024 mg/L and MIC90 (256–1024 mg/L of β-lactam antibiotics against these species. Restriction fragment length polymorphism (RFLP revealed heterogeneity in both blaA and blaB genes of Y. intermedia and Y. frederiksenii. The blaA gene of Y. intermedia shared significant sequence identity (87–96% with blaA of Y. enterocolitica biovars 1A, 1B and 4. The sequence identity of blaA of Y. frederiksenii with these biovars was 77–79%. The sequence identity of blaB gene of Y. intermedia and Y. frederiksenii was more (85% with that of Y. enterocolitica biovars 1A, 1B and 2 compared to other species viz., Y. bercovieri, Y. aldovae and Y. ruckeri. Isoelectric focusing data further revealed that both Y. intermedia and Y. frederiksenii produced Bla-A (pI 8.7 and "Bla-B like" (pI 5.5–7.1 enzymes. Conclusion Both Y. intermedia and Y. frederiksenii showed presence of blaA and blaB genes and unequivocal expression of the two β-lactamases. Limited heterogeneity

  12. Study of intermediate configurations during the fuel reload in BWRs; Estudio de configuraciones intermedias durante la recarga de combustible en BWR's

    Energy Technology Data Exchange (ETDEWEB)

    Fuentes M, L.; Castillo M, J. A.; Ortiz S, J. J.; Perusquia del C, R. [ININ, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico); Jacinto C, S., E-mail: luis.fuentes@inin.gob.mx [Universidad Autonoma del Estado de Yucatan, Calle 60 No. 491-A por 57, 97000 Merida, Yucatan (Mexico)

    2012-10-15

    The criticality state of the core of a boiling water reactor (BWR) was evaluated, during the reload process for the intermediate states between the load pattern of cycle end and the beginning of the next, using the information of the load pattern of the operation cycles 13 and 14 of Unit 1 of the nuclear power plant of Laguna Verde. For this evaluation the codes CASMO-4 and Simulate-3 for conditions of the core in cold were used. The strategy consisted on moving assemblies with 4 burned cycles of the reactor core. Later on were re situated the remaining assemblies, placing them in the positions to occupy in the next operation cycle. Finally, was carried out the assemblies load of fresh fuel. In each realized change, it was observing the behavior of the k-effective value that is the parameter used to evaluate the criticality state of each state of the core change. In a second stage, was designed a program that builds in automatic way each one of the intermediate cores and also analyzes the criticality state of the reactor core after each withdrawal, re situated and load of fuel assemblies. (Author)

  13. Zircon Carburation Studies as Intermediate Stage in the Zirconium Fabrication; Estudios encaminados a la carburacion del circon como etapa intermedia en la obtencion de circonio

    Energy Technology Data Exchange (ETDEWEB)

    Almagro Huertas, V.; Saenz de Tejada Gonzalez, L.; Lopez Rodriguez, M.

    1963-07-01

    Zirconium carbide and carbonitride mixtures were obtained by Kroll's method.Reaction products have been identified by micrography and X-ray diffraction analysis. The optimum graphite content in the initial charge for the carburation reaction has been studied. zirconium, silicon and carbon content in the final product has been controlled as a function of current in the furnace and reaction time.Further chlorination of the final product was performed successfully. (Author) 16 refs.

  14. A terapia cognitiva de Aaron Beck como reflexividade na alta modernidade: uma sociologia do conhecimento

    Directory of Open Access Journals (Sweden)

    Cláudio Ivan de Oliveira

    Full Text Available Uma sociologia do conhecimento interpreta a produção do conhecimento como vinculada a situações sociais. Partindo dessa premissa epistemológica, nosso artigo objetivou interpretar a terapia cognitiva de Aaron Beck como conhecimento construído e construtor do fenômeno de reflexividade da alta modernidade, conforme interpretada por Anthony Giddens. Nossa hipótese é que a reorientação do sistema de crenças do cliente, proposta pela terapia cognitiva, é uma forma de reconstrução reflexiva da autoidentidade, visando superar conflitos típicos da instabilidade da alta modernidade. Assinalamos aspectos na terapia cognitiva que a identificam com a reflexividade, sobretudo a ideia de que a construção da autoidentidade é uma tarefa na qual o indivíduo se engaja reflexivamente.

  15. Sobre la terapia génica para enfermedades de la retina.

    Science.gov (United States)

    Fischer, M Dominik

    2017-07-11

    Las mutaciones en un gran número de genes provocan degeneración de la retina y ceguera sin que exista actualmente cura alguna. En las últimas décadas, la terapia génica para enfermedades de la retina ha evolucionado y se ha convertido en un nuevo y prometedor paradigma terapéutico para estas enfermedades poco comunes. Este artículo refleja las ideas y los conceptos que parten de la ciencia básica hacia la aplicabilidad de la terapia génica en el ámbito clínico. Se describen los avances y las reflexiones actuales sobre la eficacia de los ensayos clínicos en la actualidad y se discuten los posibles obstáculos y soluciones de cara al futuro de la terapia génica para enfermedades de la retina. © 2017 S. Karger AG, Basel.

  16. Periodontitis in cardiovascular disease patients with or without Marfan syndrome--a possible role of Prevotella intermedia.

    Directory of Open Access Journals (Sweden)

    Jun-ichi Suzuki

    Full Text Available BACKGROUND: Although periodontitis is a risk factor for cardiovascular disease (CVD, the influence of periodontitis on Marfan syndrome (MFS with CVD is unclear. The aim of this study was to assess the relationship between periodontal bacterial burden and MSF with CVD. METHODS AND RESULTS: The subjects were patients with MFS with CVD (n = 47; age and gender matched non-MFS CVD patients (n = 48 were employed as controls. Full-mouth clinical measurements, including number of teeth, probing of pocket depth (PD, bleeding on probing (BOP and community periodontal index (CPI were recorded. We also evaluated the existence of three periodontal pathogens, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Prevotella intermedia using polymerase chain reaction assays. Serum antibody titers against the pathogens were also measured. We revealed that MFS with CVD patients had periodontitis more frequently than the age and gender matched non-MFS CVD control subjects. MFS with CVD patients had significantly severer periodontitis, fewer remaining teeth and deeper PD compared to the non-MFS CVD controls. Furthermore, the serum antibody titer level against Prevotella intermedia was significantly lower in MFS plus CVD patients compared to the non-MFS CVD patients. CONCLUSION: Periodontitis may influence the pathophysiology of cardiovascular complications in MFS patients. A specific periodontal pathogen might be a crucial therapeutic target to prevent CVD development.

  17. Isolation and characterization of Gibberella intermedia CA3-1, a novel and versatile nitrilase-producing fungus.

    Science.gov (United States)

    Wu, Yan; Gong, Jin-Song; Lu, Zhen-Ming; Li, Heng; Zhu, Xiao-Yan; Li, Hui; Shi, Jing-Song; Xu, Zheng-Hong

    2013-11-01

    Nitrilase-mediated biocatalysis has attracted substantial attention for its application in carboxylic acid production in recent years. In the present study, the fungus CA3-1 was isolated and identified as Gibberella intermedia based on its morphology, its 18S ribosomal DNA (rDNA), and internal transcribed spacer (ITS) sequences. The enzymatic properties of G. intermedia resting cells were determined, and the optimum activity was achieved at 40 °C with pH 7.6. The half-lives of the nitrilase at 30, 40, and 50 °C were 231.1, 72.9, and 6.4 h, respectively. This Gibberella nitrilase showed a wide substrate spectrum with high specificity for heterocyclic and aliphatic nitriles. It remained extremely active in 5% propanol. The presence of Ag(+), Hg(2+), and excess substrate inhibited the nitrilase activity, whereas Fe(2+), Mn(2+), and Li(+) improved enzyme activity. 3-Cyanopyridine (50 mM) was hydrolyzed into nicotinic acid within 30 min, whereas only nitrilase is a promising candidate for commercial application in nicotinic acid production. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. A Preliminary Study of the Effects of pH upon Fluorescence in Suspensions of Prevotella intermedia.

    Directory of Open Access Journals (Sweden)

    Christopher K Hope

    Full Text Available The quantification of fluorescence in dental plaque is currently being developed as a diagnostic tool to help inform and improve oral health. The oral anaerobe Prevotella intermedia exhibits red fluorescence due to the accumulation of porphyrins. pH affects the fluorescence of abiotic preparations of porphyrins caused by changes in speciation between monomers, higher aggregates and dimers, but this phenomenon has not been demonstrated in bacteria. Fluorescence spectra were obtained from suspensions of P. intermedia that were adjusted to pHs commensurate with the range found within dental plaque. Two fluorescent motifs were identified; 410 nm excitation / 634 nm emission (peak A and 398 nm excitation / 622 nm emission (peak B. A transition in the fluorescence spectra was observed from peak A to peak B with increasing pH which was also evident as culture age increased from 24 hours to 96 hours. In addition to these 'blue-shifts', the intensity of peak A increased with pH whilst decreasing with culture age from 24 to 96 hours. A bacterium's relationship with the local physiochemical environment at the time of image capture may therefore affect the quantification of dental plaque fluorescence.

  19. Effects of oxidative stress on the virulence profile of Prevotella intermedia during experimental infection in gnotobiotic mice.

    Science.gov (United States)

    Santos, Simone G; Diniz, Claúdio G; Silva, Vânia L; Martins, Wanderlany A; Cara, Denise C; Souza, Natalia C; Serufo, José C; Nicoli, Jacques R; Carvalho, Maria Auxiliadora R; Farias, Luiz M

    2007-03-01

    Prevotella intermedia is a component of the indigenous microbiota but is also responsible for anaerobic infections of the gastrointestinal tract and oral cavity. The aim of the present study was to investigate the influence of oxidative stress on the in vivo pathogenicity of P. intermedia. Germ-free mice were challenged intraperitoneally with parental (wt) or oxidative stress adapted (aero) strains. Bacterial virulence was evaluated by histopathology, hyperaemia and blood analysis [C-reactive protein (CRP), serum albumin and white blood cells (WBCs)], 3 and 10 days after challenge. CRP levels and WBC count were higher in animals challenged with the aero strain, and the albumin level was lower in this group, only 10 days after infection (P<0.05). Body weight gain was significantly reduced whereas hyperaemia and ratios of spleen/organ weight were increased in animals challenged with the aero strain (P<0.05). The liver of animals challenged with the aero strain showed hyperaemia, vasodilatation as well as an increase in the number of inflammatory cells and liver/organ weight ratio (P<0.05). Similar, but more discrete, alterations were observed in the small intestine of animals challenged with the aero strain. Studies on stress responses of this putative pathogen may help to better understand the aggressive potential and virulence markers of anaerobic bacteria.

  20. Periodontitis in cardiovascular disease patients with or without Marfan syndrome--a possible role of Prevotella intermedia.

    Science.gov (United States)

    Suzuki, Jun-ichi; Imai, Yasushi; Aoki, Mieko; Fujita, Daishi; Aoyama, Norio; Tada, Yuko; Wakayama, Kouji; Akazawa, Hiroshi; Izumi, Yuichi; Isobe, Mitsuaki; Komuro, Issei; Nagai, Ryozo; Hirata, Yasunobu

    2014-01-01

    Although periodontitis is a risk factor for cardiovascular disease (CVD), the influence of periodontitis on Marfan syndrome (MFS) with CVD is unclear. The aim of this study was to assess the relationship between periodontal bacterial burden and MSF with CVD. The subjects were patients with MFS with CVD (n = 47); age and gender matched non-MFS CVD patients (n = 48) were employed as controls. Full-mouth clinical measurements, including number of teeth, probing of pocket depth (PD), bleeding on probing (BOP) and community periodontal index (CPI) were recorded. We also evaluated the existence of three periodontal pathogens, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Prevotella intermedia using polymerase chain reaction assays. Serum antibody titers against the pathogens were also measured. We revealed that MFS with CVD patients had periodontitis more frequently than the age and gender matched non-MFS CVD control subjects. MFS with CVD patients had significantly severer periodontitis, fewer remaining teeth and deeper PD compared to the non-MFS CVD controls. Furthermore, the serum antibody titer level against Prevotella intermedia was significantly lower in MFS plus CVD patients compared to the non-MFS CVD patients. Periodontitis may influence the pathophysiology of cardiovascular complications in MFS patients. A specific periodontal pathogen might be a crucial therapeutic target to prevent CVD development.

  1. Monocytic Cell Activation by Nonendotoxic Glycoprotein from Prevotella intermedia ATCC 25611 Is Mediated by Toll-Like Receptor 2

    Science.gov (United States)

    Sugawara, Shunji; Yang, Shuhua; Iki, Koichi; Hatakeyama, Junko; Tamai, Riyoko; Takeuchi, Osamu; Akashi, Sachiko; Espevik, Terje; Akira, Shizuo; Takada, Haruhiko

    2001-01-01

    Lipopolysaccharide (LPS) preparations from gram-negative black-pigmented bacteria such as Porphyromonas gingivalis and Prevotella intermedia activate cells from non-LPS-responsive C3H/HeJ mice, but it is still unclear whether this activity is due to the unique structure of LPS or to a minor component(s) responsible for the activity in the preparation. A nonendotoxic glycoprotein with bioactivity against cells from C3H/HeJ mice was purified from a hot phenol-water extract of P. intermedia ATCC 25611 and designated Prevotella glycoprotein (PGP). Treatment of human monocytic THP-1 cells with 22-oxyacalcitriol (OCT) induced maturation and marked expression of CD14 on the cells, but the cells constitutively expressed Toll-like receptor 2 (TLR2) and TLR4 on the cells irrespective of the treatment. PGP induced a high level of interleukin-8 production at doses of 100 ng/ml and higher in OCT-treated THP-1 cells compared with Salmonella LPS, and the production was significantly inhibited by anti-CD14 and anti-TLR2 but not anti-TLR4 antibodies. Consistent with this, TLR2-deficient murine macrophages did not respond to PGP. It was also shown that PGP activity on the THP-1 cells was LPS-binding protein dependent and was inhibited by a synthetic lipid A precursor IVA. These results indicate that PGP activates monocytic cells in a CD14- and TLR2-dependent manner. PMID:11447173

  2. A Preliminary Study of the Effects of pH upon Fluorescence in Suspensions of Prevotella intermedia.

    Science.gov (United States)

    Hope, Christopher K; Billingsley, Karen; de Josselin de Jong, Elbert; Higham, Susan M

    2016-01-01

    The quantification of fluorescence in dental plaque is currently being developed as a diagnostic tool to help inform and improve oral health. The oral anaerobe Prevotella intermedia exhibits red fluorescence due to the accumulation of porphyrins. pH affects the fluorescence of abiotic preparations of porphyrins caused by changes in speciation between monomers, higher aggregates and dimers, but this phenomenon has not been demonstrated in bacteria. Fluorescence spectra were obtained from suspensions of P. intermedia that were adjusted to pHs commensurate with the range found within dental plaque. Two fluorescent motifs were identified; 410 nm excitation / 634 nm emission (peak A) and 398 nm excitation / 622 nm emission (peak B). A transition in the fluorescence spectra was observed from peak A to peak B with increasing pH which was also evident as culture age increased from 24 hours to 96 hours. In addition to these 'blue-shifts', the intensity of peak A increased with pH whilst decreasing with culture age from 24 to 96 hours. A bacterium's relationship with the local physiochemical environment at the time of image capture may therefore affect the quantification of dental plaque fluorescence.

  3. Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom

    International Nuclear Information System (INIS)

    Ullah, Anwar; Giuseppe, Priscila Oliveira de; Murakami, Mario Tyago; Trevisan-Silva, Dilza; Wille, Ana Carolina Martins; Chaves-Moreira, Daniele; Gremski, Luiza Helena; Silveira, Rafael Bertoni da; Sennf-Ribeiro, Andrea; Chaim, Olga Meiri; Veiga, Silvio Sanches; Arni, Raghuvir Krishnaswamy

    2011-01-01

    Wild-type and H12A-mutant class II phospholipase D from L. intermedia venom were crystallized; the crystals diffracted to maximum resolutions of 1.95 and 1.60 Å, respectively. Phospholipases D are the major dermonecrotic component of Loxosceles venom and catalyze the hydrolysis of phospholipids, resulting in the formation of lipid mediators such as ceramide-1-phosphate and lysophosphatidic acid which can induce pathological and biological responses. Phospholipases D can be classified into two classes depending on their catalytic efficiency and the presence of an additional disulfide bridge. In this work, both wild-type and H12A-mutant forms of the class II phospholipase D from L. intermedia venom were crystallized. Wild-type and H12A-mutant crystals were grown under very similar conditions using PEG 200 as a precipitant and belonged to space group P12 1 1, with unit-cell parameters a = 50.1, b = 49.5, c = 56.5 Å, β = 105.9°. Wild-type and H12A-mutant crystals diffracted to maximum resolutions of 1.95 and 1.60 Å, respectively

  4. Differential Midgut Attachment of Leishmania (Viannia braziliensis in the Sand Flies Lutzomyia (Nyssomyia whitmani and Lutzomyia (Nyssomyia intermedia

    Directory of Open Access Journals (Sweden)

    Rodrigo P. Soares

    2010-01-01

    Full Text Available The interaction between Leishmania and sand flies has been demonstrated in many Old and New World species. Besides the morphological differentiation from procyclic to infective metacyclic promastigotes, the parasite undergoes biochemical transformations in its major surface lipophosphoglycan (LPG. An upregulation of β-glucose residues was previously shown in the LPG repeat units from procyclic to metacyclic phase in Leishmania (Viannia braziliensis, which has not been reported in any Leishmania species. LPG has been implicated as an adhesion molecule that mediates the interaction with the midgut epithelium of the sand fly in the Subgenus Leishmania. These adaptations were explored for the first time in a species from the Subgenus Viannia, L. (V. braziliensis with its natural vectors Lutzomyia (Nyssomyia intermedia and Lutzomyia (Nyssomyia whitmani. Using two in vitro binding techniques, phosphoglycans (PGs derived from procyclic and metacyclic parasites were able to bind to the insect midgut and inhibit L. braziliensis attachment. Interestingly, L. braziliensis procyclic parasite attachment was ∼11-fold greater in the midgut of L. whitmani than in L. intermedia. The epidemiological relevance of L. whitmani as a vector of American Cutaneous Leishmaniasis (ACL in Brazil is discussed.

  5. Terapia ocupacional como instrumento nas ações de saude mental

    OpenAIRE

    Maria Jose Benetton

    1994-01-01

    Resumo: A Terapia Ocupacional tem no empirismo, muitas vezes, um elemento dificultador de sua transmissão. Ela toma o que de experimentado e pronto existe no empirismo da clínica buscando a generalização de maneira a poder demonstrar tais experimentos como pressupostos para a criação e o estabelecimento de técnicas. Como se trata da aplicabilidade das intervenções da Terapia Ocupacional na área de Saúde Mental, os procedimentos clínicos analisados foram escolhidos dentro desse universo. Com t...

  6. Prevención cardiovascular y de la osteoporosis con terapia hormonal sustitutiva

    OpenAIRE

    Martinez-Gonzalez, M.A. (Miguel Ángel); López-García, G. (Guillermo)

    1999-01-01

    Se recomienda actualmente que todo médico -sea cual sea su especialidad- aproveche la oportunidad que supone la atención clínica a cualquier mujer postmenopáusica o que se halle en torno a la menopausia para aconsejarle acerca de los beneficios y riesgos que supone la terapia hormonal sustitutiva (THS) con estrógenos y progesterona. Una vez que sea adecuadamente informada de estos beneficios y riesgos, debe ser la propia mujer quien decida si va a iniciar o no esta terapia, teniendo ...

  7. Beneficios de la terapia ecuestre en población escolar especial.

    OpenAIRE

    Ceña Ortego, Ana

    2017-01-01

    Este trabajo investiga cuáles son los beneficios de la hipoterapia o terapia ecuestre en niños en edad escolar con necesidades educativas especiales. Manifiesta la oportunidad de su uso en centros escolares, sean de integración o no. Muestra las bondades que ofrece la equino terapia como recurso educativo y como actividad extraescolar de los centros. Además ejemplifica la facilidad de coordinación y colaboración entre centros escolares y centros ecuestres. La finalidad del mismo es comprobar ...

  8. Terapias biológicas contra el cáncer

    OpenAIRE

    Barba Villanueva, Sara; Prieto Montón, Juan

    2016-01-01

    El cáncer es una de las enfermedades más importantes a nivel mundial, tanto en incidencia como en mortalidad. La terapia antineoplásica por excelencia es la quimioterapia, basada en fármacos de acción inespecífica que los hacen presentar una elevada toxicidad y efectos secundarios. Con el objetivo de solucionar estos problemas, se están desarrollando cada vez más una serie de tratamientos dirigidos diseñados para bloquear de forma específica el proceso tumoral. Así, las terapias biológicas...

  9. Terapia génica: ¿Qué es y para qué sirve?

    OpenAIRE

    Ruiz-Castellanos, M. (M.); Sangro, B. (Bruno)

    2005-01-01

    La terapia génica se ha desarrollado como un método de acercamiento al tratamiento de las enfermedades humanas basado en la transferencia de material genético a las células de un individuo. Habitualmente la finalidad de esta transferencia de material genético es restablecer una función celular que estaba abolida o defectuosa, introducir una nueva función o bien interferir con una función existente. Así, las distintas estrategias de la terapia génica se basan en la combinación de tres elemento...

  10. Prevalência de interações medicamentosas em unidades de terapia intensiva no Brasil

    OpenAIRE

    Carvalho,Rhanna Emanuela Fontenele Lima de; Reis,Adriano Max Moreira; Faria,Leila Márcia Pereira de; Zago,Karine Santana de Azevedo; Cassiani,Silvia Helena De Bortoli

    2013-01-01

    OBJETIVO: Determinar a prevalência de interações medicamentosas em unidades de terapia intensiva e analisar a significância clínica das interações identificadas. MÉTODOS: Estudo multicêntrico, transversal e retrospectivo desenvolvido com 1124 pacientes em sete unidades de terapia intensiva (UTI) de hospitais de ensino no Brasil. As informações sobre os medicamentos administrados com 24 horas e 120 horas de internação foram obtidas nas prescrições. RESULTADOS: Em 24 horas 70,6% dos pacientes a...

  11. Terapia sistémica latinoamericana: ¿Es solamente una ilusión?

    OpenAIRE

    Marcelo Rodriguez Ceberio

    2016-01-01

    RESUMEN: El artículo presenta una visión crítica pero alentadora de la terapia sistémica en Latinoamérica. Observa desde la historia de las primeras investigaciones sistémicas y sus protagonistas, hasta definir qué es la psicoterapia desde esta perspectiva. Recoge una serie de impresiones que se adentran en la terapia misma y en el rol del psicólogo sistémico, pero el análisis no queda reducido allí: se contextualiza en las diferentes realidades problemáticas que afectan a los países latinos....

  12. Terapia miofuncional en deglución atípica

    OpenAIRE

    García Veganzones, Esther

    2017-01-01

    En el presente trabajo se desarrolla un programa de intervención logopédica sobre un caso de Deglución Atípica, patología que se caracteriza fundamentalmente por un conjunto de alteraciones en las acciones musculares de la cavidad oral, asociadas generalmente a una respiración bucal. Para abordar el tratamiento de dicha alteración, se recurre a la terapia miofuncional. Con esta terapia se corrigen los desequilibrios bucofaciales que impiden realizar de manera correcta funciones como la mastic...

  13. Número de horas de cuidados de enfermagem em unidade de terapia intensiva de adultos

    OpenAIRE

    Tranquitelli,Ana Maria; Ciampone,Maria Helena Trench

    2007-01-01

    O adequado dimensionamento de pessoal de enfermagem nas Terapias Intensivas tem sido uma grande preocupação para os enfermeiros que nelas atuam a despeito dos diferentes modelos de classificação dos pacientes propostos, no âmbito nacional e internacional. No presente estudo de caso, os objetivos foram estabelecer o tempo médio despendido na assistência direta de enfermagem em uma Unidade de Terapia Intensiva geral e calcular o número médio de horas de assistência direta de enfermagem prestada...

  14. Terapia fonológica com uso de computador: relato de caso

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    Larissa Llaguno Pereira

    2013-06-01

    Full Text Available O tema desse artigo aborda as mudanças fonológicas a partir da terapia fonológica com o uso de computador em casos de desvio fonológico. Realizou-se estudo de caso de quatro sujeitos (S1, S2, S3 e S4 com idade entre 4:7 e 5:3, de ambos os gêneros, com desvio fonológico. Realizou-se a Avaliação Fonológica da Criança, Exame Articulatório Repetitivo, Avaliação do Sistema Estomatognático, Teste de Figuras para Discriminação Fonêmica, Prova de Estimulabilidade e avaliação audiológica. Os sujeitos foram divididos em duplas de acordo com a semelhança dos sistemas fonológicos, sendo que um sujeito de cada dupla foi tratado com terapia fonológica padrão e outro com uso de computador como principal recurso terapêutico (terapia experimental. Os dados das avaliações dos sujeitos foram analisados e, após, foram comparados os resultados dos submetidos à terapia padrão (S1 e S2, com os pareados submetidos à terapia experimental, com auxílio do computador, (S3 e S4 por meio do teste Exato de Fisher, considerando significância estatística de 5% (p<0,05. Verificou-se que a evolução dos sujeitos S3 e S4 submetidos à terapia experimental foi maior em relação à evolução de S1 e S2 submetidos à terapia padrão, sendo este resultado estatisticamente significante. Os achados da pesquisa demonstram terem sido favoráveis o uso da terapia experimental no tratamento de crianças com desvio fonológico. Contudo, há necessidade de realização de outros estudos com ampliação casuística para confirmação dos achados.

  15. Terapia con anticuerpos monoclonales en Cardiología y Medicina Interna

    OpenAIRE

    Christian David Adams-Sánchez; Gabriel Jaime Tobón-García

    2016-01-01

    La terapia biológica basada en anticuerpos monoclonales, constituyen una nueva herramienta terapéutica, que de la mano del desarrollo de la biología molecular permiten intervenir puntos claves en la etiopatogenia de la enfermedad, permitiendo una terapia personalizada. Se han desarrollado nuevos anticuerpos monoclonales que intervienen en las enfermedades cardiovasculares, por lo que el entendimiento de los principios terapéuticos, los tipos y el mecanismo de acción de estos fármacos es esenc...

  16. CONTRIBUIÇÕES DA TERAPIA COGNITIVO COMPORTAMENTAL NO TRATAMENTO DA OBESIDADE

    OpenAIRE

    Ana Raquel Mattoso Chagas Martins; Alice Pessanha Rocha de Assis; Amanda Barbosa de Freitas; Bianca Machado da Silva Rangel; Larissa Barreto Bastos; Erika Costa Barreto Monteiro de Barros

    2016-01-01

    Levando em consideração que o homem é o único animal capaz de modificar seu corpo através do pensamento, a Terapia Cognitivo Comportamental (TCC) vem se configurando como um importante aliado no combate à obesidade, proporcionando uma reestruturação cognitiva capaz de modificar não apenas um corpo obeso, mas modificar todo um estilo de vida. A terapia cognitivo-comportamental (TCC) tem sido amplamente usada no tratamento da obesidade em associação com outras intervenções, como o acompanhament...

  17. Trastornos de la alimentación y Terapia Ocupacional: Anorexia nerviosa

    OpenAIRE

    López Domínguez, Esther María

    2016-01-01

    Trabajo Fin de Grado Curso 2015-2016 [ES]Se realizó una revisión bibliográfica sobre la anorexia nerviosa y la terapia ocupacional, intentando analizar la evolución de este trastorno que actualmente está tan en apogeo, entre otros factores como los autores, las palabras de las cuales hacen más uso en sus títulos, el idioma y las revistas que más auge han tenido en dicho tema. Además, justificando la importancia que tiene la intervención desde la terapia ocupacional en los tr...

  18. Revisão sistemática do uso da dexametasona como terapia adjuvante na meningite bacteriana em crianças Revisión sistemática del uso de la dexametasona como terapia adyuvante en la meningitis bacteriana en niños Systematic review of dexamethasone as an adjuvant therapy for bacterial meningitis in children

    Directory of Open Access Journals (Sweden)

    João Antonio G. G. Prats

    2012-12-01

    Full Text Available OBJETIVO: Analisar a melhor evidência disponível nos últimos 15 anos com relação aos benefícios da terapia adjuvante com dexametasona na meningite bacteriana em população pediátrica. FONTES DE DADOS: Das bases de dados Medline, Lilacs e SciELO, foram analisados ensaios clínicos randomizados de 1996 a 2011, os quais comparavam a dexametasona ao placebo e/ou a outra terapia adjuvante em pacientes com meningite bacteriana diagnosticada laboratorialmente por critérios quimiocitológicos e/ou bacteriológicos, na faixa etária de 29 dias aos 18 anos. Os desfechos avaliados foram mortalidade e ocorrência de sequelas neurológicas e/ou auditivas. Foram excluídos estudos relacionados à meningite tuberculosa. SÍNTESE DOS DADOS: Com os critérios utilizados, foram identificadas cinco publicações correspondentes a quatro protocolos de estudo. Nenhum dos estudos mostrou diferenças entre a dexametasona e o placebo para os desfechos avaliados. Os estudos analisados tiveram alta qualidade (escore de Jadad et al=5. CONCLUSÕES: As evidências encontradas na literatura são insuficientes para indicar de forma rotineira o uso da dexametasona como terapia adjuvante para redução de mortalidade, perda auditiva e sequelas neurológicas em pacientes pediátricos com meningite bacteriana não tuberculosa.OBJETIVO: El presente estudio tiene por objetivo el análisis de la mejor evidencia disponible los últimos 15 años respecto a los beneficios de la terapia adyuvante con dexametasona en la meningitis bacteriana en población pediátrica por medio de revisión sistemática. FUENTES DE DATOS: De las bases de datos Medline, Lilacs y ScieLO, se analizaron ensayos clínicos aleatorios de 1996 a 2011 que comparaban la dexametasona al placebo y/u otra terapia adyuvante, en pacientes con meningitis bacteriana diagnosticada laboratorialmente por criterios quimiocitológicos y/o bacteriológicos, en la franja de edad de 29 días a 18 años. Los desenlaces

  19. MEXCODE* CASO DE ESTUDIO

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    Gabriel Arturo García Gómez

    2000-01-01

    Full Text Available El caso plantea la reactivación de un proyecto que se presentó en el año 1995 como alternativa para el desarrollo del centro del Valle y mejora de la eficiencia en el manejo del comercio exterior de la Cuenca Pacífica. El proyecto se revivió a raíz de la necesidad de la compañía patrocinadora de mejorar sus flujos de caja proyectados y la reciente autorización para su funcionamiento. El proyecto es el popularmente denominado “Puerto Seco” de la ciudad de Guadalajara de Buga, internamente nombrado Mexcode. Los estudios iniciales estaban encaminados a que el proyecto fuera manejado por el municipio de Buga, sin embargo, posteriormente se vio la necesidad de buscar que pasara a manos de la empresa privada. El municipio, después de analizar las propuestas de varios inversionistas de la región en las cuales se evaluaba la ubicación dentro de la zona de los predios de sus empresas, su área, proximidad a la línea férrea y a la doble calzada Buga-Tuluá (sitio de paso del 80% de la carga de importación y exportación por Buenaventura y evaluar aspectos relativos a su trayectoria empresarial y compromiso con el desarrollo de la región, decidió otorgarle el proyecto al Grupo Empresarial Agri-Supplies S.A.

  20. Ocorrência de acidente do trabalho em uma unidade de terapia intensiva Ocurrencia de accidentes de trabajo en una unidad de terapia intensiva The occurrence of work accidents at an intensive care unit

    Directory of Open Access Journals (Sweden)

    Vera Médice Nishide

    2004-04-01

    Full Text Available Este estudo, de caráter descritivo, identificou os acidentes do trabalho ocorridos com trabalhadores de enfermagem de uma unidade de terapia intensiva, correlacionando-os com o procedimento que estava sendo executado pelo trabalhador no momento do acidente. Os dados foram coletados por meio de entrevista individual, realizado com 68 trabalhadores, no ano de 2001. Constatou-se que os acidentes ocorreram, predominantemente, devido ao contato da pele e da mucosa com sangue e secreções, ferimento por material perfurocortante, queda e lesões na coluna vertebral. Os acidentes acontecidos estavam relacionados aos procedimentos de aspiração de tubo orotraqueal, manuseio de excretas/secreções, preparo de medicação, coleta de sangue arterial, piso molhado e transporte de paciente. Concluiu-se que são necessárias mudanças no ambiente de trabalho e programas de prevenção, para minimizar os acidentes em procedimentos de assistência aos pacientes.Este estudio, de carácter descriptivo, identificó los accidentes de trabajo ocurridos con trabajadores de enfermería de una unidad de terapia intensiva, correlacionándolos con los procedimientos que estaban siendo ejecutados por el trabajador en el momento del accidente. Los datos fueron recolectados a través de una entrevista individual, realizada con 68 trabajadores durante el ano 2001. Se verificó que los accidentes en su mayoría fueron causados por contacto de piel y mucosa con sangre y secreciones, heridas por material perforo-cortantes, caídas y lesiones en la columna vertebral. Los accidentes estaban relacionados con los procedimientos de aspiración de tubos orotraqueales, manoseo de excretas/secreciones, preparación de medicamentos, recolección de sangre arterial, piso mojado y transporte de pacientes. El estudio dejó como conclusión la necesidad de modificaciones en el ambiente de trabajo y en los programas de prevención, para disminuir los accidentes durante los procedimientos

  1. Relatives' experience of intensive care: the other side of hospitalization Vivencias de familiares en terapia intensiva: el otro lado del ingreso Vivências de familiares em terapia intensiva: o outro lado da internação

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    Urizzi Fabiane

    2007-08-01

    Full Text Available This study aims at understanding the experience concerning family members of patients in the Intensive Care Unit (ICU, with the purpose of contributing to care humanization in this context. Considering the nature of the research object, this research was carried out to understand the phenomenon Being a family that experiences the hospitalization of a family member in ICU. Phenomenology was used as a methodological reference framework. Seventeen family members of adult patients in the Intensive Care Unit (ICU at the Santa Casa in Londrina were interviewed from September to December 2004. Through analysis of these interviews, some theme categories emerged: difficult, painful, speechless experience; experiencing and recognizing somebody's life: approaching the patient's suffering; break-up of the family's daily routine; fear of having a family member die; ICU: a fearsome scene, but necessary; concern with family care. Some issues related to the family's attendance in the ICU were discussed, contributing to the establishment of humanized care delivery to critical patients and their families' uniqueness.La propuesta de este estudio es comprender las vivencias de familiares de pacientes ingresados en Unidad de Terapia Intensiva (UTI, con la intención de contribuir para la humanización del cuidado en ese contexto. Considerando la naturaleza del objeto de investigación, el estudio fue conducido en la búsqueda de la comprensión del fenómeno Ser una familia que pasa por la experiencia del ingreso de un familiar en la UTI. La fenomenología se utilizó como referencial metodológico. Se entrevistaron a 17 familias de pacientes adultos ingresados en un UTI de la Santa Casa de Londrina, de septiembre hasta diciembre de 2004. Del análisis de esas entrevistas, emergieron las categorías temáticas: experiencia difícil, dolorosa, sin palabras; ponerse en el lugar y percibir el otro: proximidad al sufrimiento del paciente; rompimiento de la relación con

  2. Spirituality in self-care for intensive care nursing professionals La espiritualidad en el cuidado de si para profesionales de enfermería en terapia intensiva A espiritualidade no cuidado de si para profissionais de enfermagem em terapia intensiva

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    Luciana Winterkorn Dezorzi

    2008-04-01

    Full Text Available This study aimed to understand how spirituality permeates the process of caring for oneself and for others in the intensive care scenario from nursing professionals' point of view. This study used the qualitative approach of Cabral's Creative-Sensitive Method to guide information production and analysis in nine art and experience workshops. Nine nursing caregivers from the Intensive Care Unit (ICU of a university hospital participated in the study. This article presents one of the topics that emerged during this process: spirituality in self-care, which is evidenced in the daily practices that take place through prayers, close contact with nature, as well as in the sense of connection with a Higher Power that provides peace, welfare, and greater strength to ICU caregivers' life and work. Self-knowledge emerged as an essential practice in caring for oneself, in order to deliver better care to others.Este estudio tuvo por objetivo comprender como la espiritualidad envuelve al proceso de cuidar de sí mismo y de otros, en el mundo de la terapia intensiva, bajo la perspectiva de los profesionales de enfermería. La investigación se caracterizó por un abordaje cualitativo del método creativo sensible de Cabral, que guió la producción y el análisis de las informaciones en nueve talleres de arte y experiencias. Participaron del estudio nueve cuidadoras de enfermería del Centro de Tratamiento Intensivo (CTI de un hospital universitario. Este artículo presenta uno de los temas que surgieron en la investigación: la espiritualidad en el cuidado de sí mismo, la que fue evidenciada en las prácticas cotidianas que acontecían por medio de la oración, del contacto íntimo con la naturaleza, así como en el sentimiento de conexión con una Fuerza Superior que propiciaba tranquilidad, bienestar y fortalecía la vida y el trabajo de las cuidadoras en el CTI. El auto conocimiento se reveló como una práctica esencial en el cuidado de sí mismo que

  3. Estenosis traqueal: Estudio preliminar

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    Rafael Pinilla González

    2000-12-01

    Full Text Available Se realizó un estudio retrospectivo y descriptivo con la utilización de los expedientes clínicos de 5 pacientes con estenosis traqueal, mayoritariamente como consecuencia de intubación prolongada, y en el que se tuvo en cuenta aspectos relacionados con el diagnóstico, el tratamiento y las complicaciones. Existió un predominio del sexo femenino y edad media de 44,6 años. La intubación prolongada de origen diverso fue la causa de la estenosis en 4 de los 5 pacientes y en 1 fue posterior a la reparación de lesión traqueal yatrógena. La instalación de la estenosis tuvo lugar entre 8 días y 3 meses. La tomografía lineal en 3 pacientes y en 2 la radiografía simple de tráquea anteroposterior y lateral verificaron el diagnóstico. La endoscopia traqueobronquial preoperatoria fue de utilidad en la caracterización de la estenosis. En todos los pacientes se realizó tratamiento quirúrgico al resecar el área estenótica con posterior anastomosis terminoterminal y fijación del cuello en flexión durante 8 días como promedio. Dos pacientes mostraron granulomas en el nivel de la línea de sutura, los cuales fueron tratados por vacuolización con láser. No se reportó mortalidad en la muestra estudiadaA retrospective and descriptive study was conducted by using the medical histories of 5 patients with tracheal stenosis, mostly as a result of prolongued intubation. Aspects connected with the diagnosis, treatment and complications were taken into account. It was observed a predominance of females and an average age of 44.6. Prolongued intubation of different origin was the cause of stenosis in 4 of the 5 patients, whereas in the other it occurred after the reparation of the tracheal and athrogenous lesion. The installation of stenosis took place between 8 days and 3 months. Lineal tomography in 3 patients and anteroposterior and lateral simple X-ray of the trachea in 2 confirmed the diagnosis. Preoperative tracheobronchial endoscopy was

  4. TERAPIAS COMPLEMENTARIAS Y ALTERNATIVAS EN VIH/SIDA

    OpenAIRE

    BRAVO GONZÁLEZ,VICTORIA; VALDEBENITO COT,FRANCISCA; CIANELLI ACOSTA,ROSINA; FERRER LAGUNAS,LILIAN; IRARRÁZABAL VARGAS,LISETTE

    2009-01-01

    A pesar de ser una prioridad mundial, ONUSIDA en el 2004 reporta que sólo un 10% de las personas viviendo con VIH o SIDA en el mundo tiene acceso a los medicamentos antirretrovirales (TAR), único tratamiento que ha demostrado disminución de la mortalidad de las personas que viven con VIH y SIDA. A pesar de ello, la utilización de TAR conlleva efectos adversos inmediatos y mediatos que conducen a otras patologías crónicas. El presente estudio bibliográfico describe la literatura disponible sob...

  5. TERAPIA GÉNICA Y PRINCIPIOS ÉTICOS A TERAPIA GÊNICA E OS PRINCÍPIOS ÉTICOS GEN THERAPY AND ETHICAL PRINCIPLES

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    Eduardo Rodríguez Yunta

    2003-01-01

    Full Text Available La terapia génica constituye una forma de manipulación genética que trata de corregir o disminuir los efectos que ocasionan enfermedades de origen genético. Existen grandes esperanzas en el desarrollo de este tipo de terapia, sobre todo para enfermedades somáticas de origen genético que no tienen curación. Pero hay también numerosas dificultades técnicas que no están del todo resueltas. No se ha demostrado todavía eficacia clínica y se pueden producir daños irreversibles en el organismo. Por una parte, existe la necesidad de regular por medio de protocolos que deben ser rigurosamente analizados por comités de evaluación ética y científica y, por otra, hace falta un diálogo entre países, con el fin de evitar una presentación exitista de la técnica en el mercado sin mencionar sus dificultades inherentes. Una de las preocupaciones mayores es que la aceptación paulatina de este tipo de terapia, y la eliminación de restricciones, permita el uso de la técnica para terapia génica germinal y para terapia génica de mejoría, cuya validez ética es cuestionada. Usar la terapia génica en células germinales conlleva el riesgo de introducir daños genéticos en generaciones posterioresA terapia gênica constitui uma forma de manipulação genética que trata de corrigir ou diminuir os efeitos que ocasionam enfermidades de origem genética. Existem grandes esperanças no desenvolvimento deste tipo de terapia, sobretudo para doenças somáticas de origem genética que não tem cura. Existem, não obstante isso numerosos dificuldades técnicas que não estão totalmente resolvidas. Não se demostrou ainda a eficácia clínica e se pode causar danos irreversíveis no organismo. De um lado, existe a necessidade de regular por meio de protocolos que devem ser rigorosamente analisados por comitês de avaliação ética e científica, por outra, faz falta um diálogo entre países, para se evitar uma apresentação unilateral exitosa da t

  6. Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts fromEphedra intermediaIndigenous to Balochistan.

    Science.gov (United States)

    Gul, Rahman; Jan, Syed Umer; Faridullah, Syed; Sherani, Samiullah; Jahan, Nusrat

    2017-01-01

    The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the E. intermedia to prove its uses in Pakistani folk medicines for the treatment of asthma and bronchitis. Antioxidant activity was analyzed by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay. Standard methods were used for the identification of cardiac glycosides, phenolic compounds, flavonoids, anthraquinones, and alkaloids. High performance liquid chromatography (HPLC) was used for quantitative purpose of ephedrine alkaloids in E. intermedia . The quantitative separation was confirmed on Shimadzu 10AVP column (Shampack) of internal diameter (id) 3.0 mm and 50 mm in length. The extract of the solute in flow rate of 1 ml/min at the wavelength 210 nm and methanolic extract showed the antioxidant activity and powerful oxygen free radicals scavenging activities and the IC50 for the E. intermedia plant was near to the reference standard ascorbic acid. The HPLC method was useful for the quantitative purpose of ephedrine (E) and pseudoephedrine (PE) used for 45 samples of one species collected from central habitat in three districts (Ziarat, Shairani, and Kalat) of Balochistan. Results showed that average alkaloid substance in E. intermedia was as follows: PE (0.209%, 0.238%, and 0.22%) and E (0.0538%, 0.0666%, and 0.0514%).

  7. Use of PCR and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Techniques for Differentiation of Prevotella intermedia Sensu Stricto and Prevotella nigrescens

    Science.gov (United States)

    Premaraj, Thyagaseely; Kato, Naoki; Fukui, Katsuhito; Kato, Haru; Watanabe, Kunitomo

    1999-01-01

    Primers were designed from 16S rRNA sequences of Prevotella intermedia sensu stricto and Prevotella nigrescens and were used to discriminate these two species by PCR. The results were compared with those from the PCR technique using primers designed from arbitrarily primed PCR products by Guillot and Mouton (E. Guillot and C. Mouton, J. Clin. Microbiol. 35:1876–1882, 1997). The specificities of both assays were studied by using P. intermedia ATCC 25611, P. nigrescens ATCC 33563, 174 clinical isolates of P. intermedia sensu lato, and 59 reference strains and 58 clinical isolates of other Prevotella species and/or common oral flora. In addition, the usefulness and reliability of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in the differentiation of the two species were examined by comparing the results with those from PCR assays. The controversial lipase test for distinguishing these species was also carried out. Unambiguous differentiation was made by both PCR assays, and the results matched each other. The SDS-PAGE assay was found to misidentify a few strains tested, compared with the results of PCR assays. The lipase test was positive for both species, including the reference strains of P. intermedia and P. nigrescens. We conclude that both PCR assays are simple, rapid, reliable, and specific methods which could be used in clinical studies and that the lipase test is not valuable in the differentiation. The reliable discrimination of the two species by SDS-PAGE is questionable. PMID:10074526

  8. Draft Whole-Genome Sequences of Periodontal PathobiontsPorphyromonas gingivalis,Prevotella intermedia, andTannerella forsythiaContain Phase-Variable Restriction-Modification Systems.

    Science.gov (United States)

    Haigh, Richard D; Crawford, Liam A; Ralph, Joseph D; Wanford, Joseph J; Vartoukian, Sonia R; Hijazi, Karolin; Wade, William; Oggioni, Marco R

    2017-11-16

    Periodontal disease comprises mild to severe inflammatory host responses to oral bacteria that can cause destruction of the tooth-supporting tissue. We report genome sequences for 18 clinical isolates of Porphyromonas gingivalis , Prevotella intermedia , and Tannerella forsythia , Gram-negative obligate anaerobes that play a role in the periodontal disease process. Copyright © 2017 Haigh et al.

  9. FLOW CHAMBER STUDY OF THE ADHESION OF PREVOTELLA-INTERMEDIA TO GLASS AFTER PRECONDITIONING WITH MUTANS STREPTOCOCCAL SPECIES - KINETICS AND SPATIAL ARRANGEMENT

    NARCIS (Netherlands)

    COWAN, M; BUSSCHER, HJ

    1993-01-01

    The adhesion kinetics and spatial arrangement of a clinical isolate of Prevotella intermedia on bare glass and on glass which had been previously exposed to Streptococcus rattus or Streptococcus cricetus were studied using a parallel plate flow system and image analysis. When S. cricetus was the

  10. Cultura de seguridad del paciente en la prestación de servicios profesionales de Terapia Ocupacional

    Directory of Open Access Journals (Sweden)

    Olga Peñas Felizzola

    2017-01-01

    Full Text Available Objetivo: Caracterizar el ambiente de seguridad del paciente en un servicio de Terapia Ocupacional donde se desarrollan actividades docentes y asistenciales para el programa de la Universidad Nacional de Colombia. Materiales y m étodos : Estudio descriptivo transversal, con aplicación de la Encuesta sobre clima de seguridad del paciente en servicios del área de la salud (versión para Colombia, adaptada y en formato electrónico, a tres terapeutas ocupacionales vinculadas durante 2013 a 2015 en un escenario docente asistencial de la Universidad Nacional de Colombia. Consulta de reportes y estadísticas del servicio correspondientes al periodo de análisis. Resultados: Entre los hallazgos de la encuesta resaltan el subregistro de eventos, aunque no haya una percepción punitiva frente a su reporte; se percibe comunicación abierta, con necesidad de mejorar la retroalimentación ante los errores; considerable rotación e insuficiencia de personal; se aprecia apoyo institucional al tema; y se evalúa como positivo el aprendizaje organizacional y mejoramiento continuo del servicio. Conclusiones: En términos globales, las profesionales valoran bien el ambiente de seguridad del paciente en el servicio analizado, pero debe trabajarse en pro de los aspectos claves que eviden - ciaron debilidades en las categorías analizadas, donde preocupa la aparente falta de registro de eventos y la consiguiente debilidad para establecer medidas correctivas. Además, es importante capitalizar estas experiencias y procesos sobre gestión de la calidad y seguridad del paciente, articulando con las actividades de formación y docencia que también se llevan a cabo allí.

  11. Uso de terapia antirretroviral de gran actividad (TARGA y transmisión vertical del VIH. Cuba 1989-2008

    Directory of Open Access Journals (Sweden)

    Tania Roig Álvarez

    2013-06-01

    Full Text Available OBJETIVOS: describir el uso de terapia antirretroviral de gran actividad (TARGA en gestantes cubanas infectadas por el VIH y el comportamiento de la transmisión vertical del VIH, según tratamiento antirretroviral materno usado durante la gestación. MÉTODOS: se realizó un estudio de corte transversal donde se incluyeron 319 niños hijos de mujeres seropositivas al VIH, identificándose en ellos la presencia de infección o no por el VIH; para el análisis se utilizaron medidas descriptivas, la prueba de comparación de tasas y la razón de prevalencia. RESULTADOS: la transmisión vertical del VIH de forma general fue de 5% y esta fue mayor en los años en que no se usaba TARGA (9,5% vs 3,1%; p=0,049. Inicialmente solo recibían TARGA gestantes clasificadas como sida. En el transcurso del tiempo se observó un incremento del uso de este esquema de tratamiento en gestantes y una disminución en la infección de la descendencia (26,3% en 2003 vs 86,7% en 2008 y 10,5% en 2003 vs 2,1% en 2008; respectivamente. La prevalencia de la enfermedad disminuyó al usar un esquema de tratamiento materno de mayor actividad (tendencia lineal p=0,017. CONCLUSIONES: el perfeccionamiento del programa de prevención cubano ha disminuido la transmisión vertical del VIH.

  12. Efecto de la terapia antiretroviral de gran actividad (TARGA en pacientes enrolados en un Hospital Público en Lima-Perú.

    Directory of Open Access Journals (Sweden)

    Juan Echevarría Zárate

    2007-10-01

    Full Text Available Introducción: En mayo del 2004, el Ministerio de Salud (MINSA en colaboración con el Fondo Global de lucha contra el SIDA, la tuberculosis y la malaria implementó el programa nacional (PN para brindar terapia antiretroviral en forma gratuita a todos los pacientes infectados con el VIH con indicación de tratamiento de acuerdo a la guía del PN. Objetivos: Describir las características pre-tratamiento antiretroviral, respuesta a la terapia antiretroviral de gran actividad (TARGA, toxicidad y tasa de mortalidad de la población de pacientes que iniciaron TARGA a través del PN en un hospital general. Material y Métodos: Revisamos las historias clínicas, fichas médicas, resultados de laboratorio y tarjetas de TARGA de todos los pacientes enrolados en el PN en el Hospital Nacional Cayetano Heredia (HNCH y describimos las características pre-tratamiento antiretroviral, respuesta a la TARGA, toxicidad y tasa de mortalidad. Los pacientes seleccionados para este estudio fueron sujetos infectados con el VIH que: (i tenían 18 años o más; (ii iniciaron TARGA en el PN entre mayo 2004 y abril 2006; y (iii no habían recibido terapia antiretroviral anteriormente (naïve. Se consideró como éxito virológico a la presencia de por lo menos un valor de carga viral indetectable (VIH-1 ARN < 400 copias/mL entre los 3-6 meses posteriores al inicio de la TARGA. Resultados: Se incluyeron en el estudio a 453 pacientes: 68% fueron varones, la edad promedio fue 35,7 ± 9,5 años y el peso fue 55,6 ± 10,8 Kg. El valor medio de CD4 pre-tratamiento fue 98 células/mm3 y el de carga viral fue 278 438 VIH-1 ARN copias/mL. Descontinuaron el tratamiento 84 pacientes (22 abandonaron, 59 fallecieron y 3 fueron transferidos a otra institución. Aquellos que continuaron fueron seguidos en promedio 337 días; el 38% (142/369 interrumpió temporalmente la TARGA, principalmente debido a toxicidad medicamentosa (82%. Se obtuvo éxito virológico en 85% de los pacientes

  13. Diseño de un modelo de historia clínica para terapia neural acorde con la reglamentación actual en Colombia / Design of a model of clinical history for neural therapy according to the current regulation in Colombia

    OpenAIRE

    Segura Chaves, Paulo Renato

    2010-01-01

    Objetivo: Presentar un diseño de historia clínica que incluya los diferentes principios de la Terapia Neural según Huneke teniendo en cuenta la actual legislación colombiana. Metodología: por medio de un estudio descriptivo se diseñó un modelo inicial de historia clínica que fue sometido a evaluación por parte de expertos permitiendo la realización del diseño final. Resultados: El diseño final cumplió con las características de integralidad, funcionalidad, secuencialidad, seguridad, utilidad,...

  14. Efecto de la aplicación de terapia conductual en la adquisición de destrezas básicas para el aprendizaje, en tres niños con autismoa partir de la expresión artística

    OpenAIRE

    Hannia Cabezas Pizarro

    2004-01-01

    El presente estudio muestra el efecto de la utilización de terapia conductual aplicada, en tres niños con autismo, y cuyo propósito fue el de desarrollar aquellas conductas de entrada básicas para el aprendizaje como son el permanecer sentados, el control de llanto, el establecimiento de contacto ocular y la identificación del yo, consideradas habilidades básicas que deben tener los niños para incursionar en el proceso educativo. Una vez que el niño adquiere esta gama ...

  15. Prevalencia del tratamiento adjunto con antipsicóticos en pacientes adultos predominantemente latinos con depresión no psicótica: Un estudio retrospectivo.

    OpenAIRE

    Abulseoud, Osama A; Department of Psychiatry and Behavioral sciences, Keck School of Medicine at the University of Southern California, Los Angeles, CA 90033. USA Department of Psychiatry and Psychology, Mayo Clinic College of Medicine, Rochester, MN 55905.; Costales, Jesse; Department of Psychiatry and Behavioral sciences, Keck School of Medicine at the University of Southern California, Los Angeles, CA 90033.; Peterson, Melanie; Department of Psychiatry and Behavioral sciences, Keck School of Medicine at the University of Southern California, Los Angeles, CA 90033.; Miller, Joseph D; Department of Cell and Neurobiology Keck School of Medicine at University of Southern California, Los Angeles, CA 90033. USA Department of Pharmacology, American University of the Caribbean School of Medicine.; Lagomasino, Isabel T; Department of Psychiatry and Behavioral sciences, Keck School of Medicine at the University of Southern California, Los Angeles, CA 90033.

    2014-01-01

    El uso de antipsicóticos atípicos como terapia adjunta a antidepresivos en el tratamiento del trastorno depresivo mayor (TDM) no psicótico fue práctica común por un largo periodo antes de que los ensayos clínicos a doble-ciego, controlados (con placebo) y al azar, llevados a cabo a gran escala, demostraran su eficacia. El presente estudio se propuso evaluar la frecuencia con la cual pacientes diagnosticados con TDM recibieron tratamiento con agentes antipsicóticos (AP) y examinar los efectos ...

  16. CONTRIBUIÇÕES DA TERAPIA COGNITIVO COMPORTAMENTAL NO TRATAMENTO DA OBESIDADE

    Directory of Open Access Journals (Sweden)

    Ana Raquel Mattoso Chagas Martins

    2016-08-01

    Full Text Available Levando em consideração que o homem é o único animal capaz de modificar seu corpo através do pensamento, a Terapia Cognitivo Comportamental (TCC vem se configurando como um importante aliado no combate à obesidade, proporcionando uma reestruturação cognitiva capaz de modificar não apenas um corpo obeso, mas modificar todo um estilo de vida. A terapia cognitivo-comportamental (TCC tem sido amplamente usada no tratamento da obesidade em associação com outras intervenções, como o acompanhamento nutricional e a prática de atividade física. A TCC é uma terapia de duração breve e com sessões estruturadas nas quais o paciente é solicitado a colaborar ativamente na identificação e na mudança de seus comportamentos e sentimentos que contribuem para a obesidade. O objetivo do presente trabalho é verificar a eficácia do apoio psicoterápico com base na Terapia Cognitivo Comportamental na redução de peso de pacientes obesos. Palavras-chave: Cognitivo; Comportamental; Obesidade.

  17. Atitude de Estudantes de Medicina frente a Terapias Alternativas e Complementares

    Directory of Open Access Journals (Sweden)

    Nayara Mendes Morales

    Full Text Available O uso das Terapias Alternativas e Complementares (TAC vem aumentando ao longo dos anos no Brasil e no mundo com o advento de políticas de saúde favoráveis ao emprego dessas práticas. No entanto, as escolas médicas ainda não oferecem disciplinas curriculares sobre este tema. Com o intuito de descrever as atitudes dos estudantes de Medicina de uma universidade do Sul do Brasil com relação às terapias complementares, foi realizado um estudo observacional transversal descritivo, por meio da aplicação de um questionário. Houve 65,22% de taxa de resposta, e a terapia mais conhecida pelos estudantes foi a Fitoterapia (90,3%. A maioria deles demonstrou atitudes favoráveis com relação às terapias, e 75,26% gostariam de aprender sobre o tema em aulas curriculares.

  18. Terapia comportamental cognitiva para pessoas com transtorno bipolar Cognitive behavioral therapy for bipolar disorders

    Directory of Open Access Journals (Sweden)

    Francisco Lotufo Neto

    2004-10-01

    Full Text Available Descrição dos objetivos e principais técnicas da terapia comportamental cognitiva usadas para a psicoterapia das pessoas com transtorno bipolar.Objectives and main techniques of cognitive behavior therapy for the treatment of bipolar disorder patients are described.

  19. Uso de kits desechables para optimizar tiempos, movimientos y apego a protocolos de la terapia intravenosa

    Directory of Open Access Journals (Sweden)

    José Juan Escamilla Zamudio

    2017-09-01

    Cómo citar este artículo: Escamilla JJ, Ramírez GI. Uso de kits desechables para optimizar tiempos, movimientos y apego a protocolos de la terapia intravenosa. Rev Cuid. 2017; 8(3: 1749-57. http://dx.doi.org/10.15649/cuidarte.v8i3.412

  20. Arte africano como punto de partida para una actividad de arte terapia

    OpenAIRE

    Vassiliadou Yiannaka, María

    2001-01-01

    La visita a una exposición de arte africano realizada con un grupo de pacientes de un Hospital Psiquiátrico sirve en este artículo para reflexionar sobre los aspectos implicados en la organización de actividades de arte terapia y sobre el problema de la accesibilidad de todos los ciudadanos a la vida cultural

  1. NCX 4040, a nitric oxide-donating aspirin derivative, inhibits Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages.

    Science.gov (United States)

    Choi, Eun-Young; Choe, So-Hui; Hyeon, Jin-Yi; Park, Hae Ryoun; Choi, Jeom-Il; Choi, In Soon; Kim, Sung-Jo

    2015-12-05

    In this study, the effects and underlying mechanisms of NCX 4040, a nitric oxide (NO)-donating aspirin derivative, on the production of proinflammatory mediators were examined using murine macrophages exposed to lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen implicated in the etiology of periodontal disease. NCX 4040 significantly reduced P. intermedia LPS-induced production of inducible NO synthase (iNOS)-derived NO, IL-1β and IL-6 as well as their mRNA expression in RAW264.7 cells. Notably, NCX 4040 was much more effective than the parental compound aspirin in reducing LPS-induced production of inflammatory mediators. NCX 4040 induced the expression of heme oxygenase-1 (HO-1) in cells treated with P. intermedia LPS, and the suppressive effect of NCX 4040 on LPS-induced NO production was significantly reversed by SnPP, a competitive HO-1 inhibitor. NCX 4040 did not influence LPS-induced phosphorylation of JNK and p38. IκB-α degradation as well as nuclear translocation and DNA-binding activities of NF-κB p65 and p50 subunits induced by P. intermedia LPS were significantly reduced by NCX 4040. Besides, LPS-induced phosphorylation of STAT1 and STAT3 was significantly down-regulated by NCX 4040. Further, NCX 4040 elevated the SOCS1 mRNA in cells stimulated with LPS. This study indicates that NCX 4040 inhibits P. intermedia LPS-induced production of NO, IL-1β and IL-6 in murine macrophages through anti-inflammatory HO-1 induction and suppression of NF-κB, STAT1 and STAT3 activation, which is associated with the activation of SOCS1 signaling. NCX 4040 could potentially be a promising tool in the treatment of periodontal disease, although further studies are required to verify this. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Evaluación de la terapia mecánica periodontal en bolsas profundas: Respuesta clínica y bacteriológica Evaluation of periodontal mechanical therapy in deep pockets: Clinical and bacteriological response

    Directory of Open Access Journals (Sweden)

    G Bazzano

    2012-12-01

    Full Text Available Objetivos: Evaluar la composición microbiológica y los parámetros clínicos de bolsas periodontales ≥5 mm de profundidad al inicio, 1 semana, 3 y 12 meses post raspado y alisado radicular. Materiales y Métodos: Se tomaron registros clínicos y muestras de placa subgingival de 44 sitios de pacientes con diagnóstico de periodontitis crónica. Se identificaron por técnica de Reacción en Cadena de la Polimerasa (PCR patógenos putativos periodontales: Aggregatibacter actinomycetemcomitans (Aa, Porphyromonas gingivalis (Pg, Treponema denticola (Td, Tannerella forsythia (Tf y Prevotella intermedia (Pi. Los pacientes recibieron terapia mecánica periodontal y fueron reevaluados a los 7 días, 3 y 12 meses. Resultados: Luego del tratamiento, todos los parámetros clínicos (Placa Bacteriana, Hemorragia, Supuración, Profundidad al Sondaje y Nivel de Inserción Clínica se redujeron significativamente y los valores obtenidos se mantuvieron hasta los 12 meses. Al inicio, las especies bacterianas prevalentes fueron Pg, presente en 66% de los sitios, Tf (55% y Td (41%. Los sitios más profundos se relacionaron con las asociaciones Tf-Td (6.8 mm y Tf-Td-Pi (7 mm. Post terapia, el número de sitios positivos para Td, Tf y Pg se redujo significativamente. Conclusiones: El raspado y alisado radicular mejoró significativamente los parámetros clínicos y redujo la prevalencia de los patógenos periodontales Pg, Tf y Td en bolsas periodontales profundas. Los resultados obtenidos se mantuvieron hasta los 12 meses. No se detectaron mayores pérdidas de inserción clínica en el 86% de los sitios a 3 meses y en 79% a los 12 meses. Los sitios en los que el tratamiento no fue efectivo en la eliminación de patógenos a los 12 meses desarrollaron mayores profundidades de sondaje.Objectives: To evaluate the microbial composition and clinical parameters of periodontal pockets with probing depth ≥5 mm at baseline, 1 week, 3 and 12 months after scaling and root

  3. Eficácia da terapia cognitiva processual no transtorno de estresse pós-traumático

    OpenAIRE

    Érica Panzani Duran

    2016-01-01

    Objetivo: Avaliar a eficácia da Terapia Cognitiva Processual na melhora de pacientes com Transtorno de Estresse Pós-traumático. Desenho do estudo - Estudo randomizado, com dois grupos paralelos. Intervenções - Dois modelos de Terapia Comportamental Cognitiva - Terapia Cognitiva Processual e Exposição (os pacientes receberam tratamento farmacológico). Duração e frequência: Após a avaliação inicial (semana 00), as sessões de psicoterapia tiveram frequência semanal, durante onze semanas, e quinz...

  4. A persistent cough as atypical clinical presentation of intrathoracic extramedullary hematopoiesis (EMH) in a female with thalassemia intermedia.

    Science.gov (United States)

    Abdulla, Mohammad Aj; Yassin, Mohamed A; Abdelrazek, Mohamed; Mudawi, Deena; Ibrahim, Firyal; Soliman, Dina S; ElOmri, Halima; Nashwan, Abdulqadir J; Fernihough, Liam; De Sanctis, Vincenzo; Soliman, Ashraf T

    2018-02-16

    Extramedullary hematopoiesis (EMH) is a rare disorder, defined as the appearance of hematopoietic elements outside the bone marrow or peripheral blood. The most common sites of EMH are liver and spleen, but it has been documented in other organs such as the mediastinum, lymph nodes, breast, and central nervous system. EMH occurs as a compensatory mechanism for bone marrow dysfunction in severe thalassemia. We report a case of EMH presenting as a posterior mediastinal mass in a 34-year-old woman with thalassemia intermedia with chronic cough and shortness of breath on exertion. The diagnosis of EMH was confirmed by a CT-guided fine needle biopsy. All symptoms disappeared after surgical removal of the mass.

  5. Ethanol and Protein from Ethanol Plant By-Products Using Edible Fungi Neurospora intermedia and Aspergillus oryzae.

    Science.gov (United States)

    Bátori, Veronika; Ferreira, Jorge A; Taherzadeh, Mohammad J; Lennartsson, Patrik R

    2015-01-01

    Feasible biorefineries for production of second-generation ethanol are difficult to establish due to the process complexity. An alternative is to partially include the process in the first-generation plants. Whole stillage, a by-product from dry-mill ethanol processes from grains, is mostly composed of undegraded bran and lignocelluloses can be used as a potential substrate for production of ethanol and feed proteins. Ethanol production and the proteins from the stillage were investigated using the edible fungi Neurospora intermedia and Aspergillus oryzae, respectively. N. intermedia produced 4.7 g/L ethanol from the stillage and increased to 8.7 g/L by adding 1 FPU of cellulase/g suspended solids. Saccharomyces cerevisiae produced 0.4 and 5.1 g/L ethanol, respectively. Under a two-stage cultivation with both fungi, up to 7.6 g/L of ethanol and 5.8 g/L of biomass containing 42% (w/w) crude protein were obtained. Both fungi degraded complex substrates including arabinan, glucan, mannan, and xylan where reductions of 91, 73, 38, and 89% (w/v) were achieved, respectively. The inclusion of the current process can lead to the production of 44,000 m(3) of ethanol (22% improvement), around 12,000 tons of protein-rich biomass for animal feed, and energy savings considering a typical facility producing 200,000 m(3) ethanol/year.

  6. Interleukin 10-Dominant Immune Response and Increased Risk of Cutaneous Leishmaniasis After Natural Exposure to Lutzomyia intermedia Sand Flies.

    Science.gov (United States)

    Carvalho, Augusto M; Cristal, Juqueline R; Muniz, Aline C; Carvalho, Lucas P; Gomes, Regis; Miranda, José C; Barral, Aldina; Carvalho, Edgar M; de Oliveira, Camila I

    2015-07-01

    Leishmaniasis is caused by parasites transmitted to the vertebrate host by infected sand flies. During transmission, the vertebrate host is also inoculated with sand fly saliva, which exerts powerful immunomodulatory effects on the host's immune response. We conducted a prospective cohort analysis to characterize the human immune response to Lutzomyia intermedia saliva in 264 individuals, from an area for cutaneous leishmaniasis (CL) caused by Leishmania braziliensis. Antibodies were found in 150 individuals (56.8%); immunoglobulin G1 and G4 were the predominant subclasses. Recall responses to salivary gland sonicate showed elevated production of interleukin 10 (IL-10), interleukin 13, interferon γ, CXCL9, and CCL2 compared with controls. CD4(+)CD25(+) T cells, including Foxp3(+) cells, were the main source of IL-10. L. braziliensis replication was increased (P < .05) in macrophages cocultured with saliva-stimulated lymphocytes from exposed individuals and addition of anti-IL-10 reverted this effect. Positive correlation between antibody response to saliva and cellular response to Leishmania was not found. Importantly, individuals seropositive to saliva are 2.1 times more likely to develop CL (relative risk, 2.1; 95% confidence interval, 1.07-4.2; P < .05). Exposure to L. intermedia sand flies skews the human immune response, facilitating L. braziliensis survival in vitro, and increases the risk of developing CL. © The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  7. Ethanol and Protein from Ethanol Plant By-Products Using Edible Fungi Neurospora intermedia and Aspergillus oryzae

    Directory of Open Access Journals (Sweden)

    Veronika Bátori

    2015-01-01

    Full Text Available Feasible biorefineries for production of second-generation ethanol are difficult to establish due to the process complexity. An alternative is to partially include the process in the first-generation plants. Whole stillage, a by-product from dry-mill ethanol processes from grains, is mostly composed of undegraded bran and lignocelluloses can be used as a potential substrate for production of ethanol and feed proteins. Ethanol production and the proteins from the stillage were investigated using the edible fungi Neurospora intermedia and Aspergillus oryzae, respectively. N. intermedia produced 4.7 g/L ethanol from the stillage and increased to 8.7 g/L by adding 1 FPU of cellulase/g suspended solids. Saccharomyces cerevisiae produced 0.4 and 5.1 g/L ethanol, respectively. Under a two-stage cultivation with both fungi, up to 7.6 g/L of ethanol and 5.8 g/L of biomass containing 42% (w/w crude protein were obtained. Both fungi degraded complex substrates including arabinan, glucan, mannan, and xylan where reductions of 91, 73, 38, and 89% (w/v were achieved, respectively. The inclusion of the current process can lead to the production of 44,000 m3 of ethanol (22% improvement, around 12,000 tons of protein-rich biomass for animal feed, and energy savings considering a typical facility producing 200,000 m3 ethanol/year.

  8. Detection of Porphyromonas endodontalis, Porphyromonas gingivalis and Prevotella intermedia in primary endodontic infections in a Chinese population.

    Science.gov (United States)

    Cao, H; Qi, Z; Jiang, H; Zhao, J; Liu, Z; Tang, Z

    2012-08-01

    To assess the prevalence of three black-pigmented bacterial species (Porphyromonas endodontalis, Porphyromonas gingivalis and Prevotella intermedia) using microarray technology in root canals of teeth associated with primary endodontic infections in a Chinese population. Microbial samples were taken from root canals of 80 teeth with pulp necrosis and primary endodontic infections in a Chinese population. DNA extracted from the samples was amplified by PCR with universal bacterial primers based on 16S rRNA gene sequences, and the products hybridized with the microarrays in which the specific oligonucleotide probes were added. The results of hybridization were screened by a confocal laser scanner. Pearson chi-square test and the two-sided Fisher exact test were used to analyse whether a significant association existed between the species and symptoms as well as in co-existence of two target organisms by a statistical software package (SAS 8.02). The 16S rRNA gene microarray detected at least one of the three test species in 76% of the study teeth. P. endodontalis, P. gingivalis and P. intermedia were found in 50%, 33% and 45%, respectively. A significant association was found in the presence of the pair P. endodontalis / P. gingivalis (P < 0.005). Both P. endodontalis (P <0.05) and P. gingivalis (P <0.005) had a statistically significant association with the presence of a sinus tract. The simultaneous presence of P. endodontalis and P. gingivalis was also associated with the presence of a sinus tract (P<0.005) and abscess formation (P<0.05). The three black-pigmented bacteria were prevalent in teeth with pulp necrosis and primary endodontic infections in a Chinese population. P. gingivalis and P. endodontalis were associated with the presence of sinus tract and abscess formation. © 2012 International Endodontic Journal.

  9. Bases da terapia celular em cardiologia Cell therapy in cardiology

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    Antonio Carlos C. Carvalho

    2009-05-01

    Full Text Available O implante de células para o tratamento de doenças cardiovasculares encontra-se sob investigação em vários centros no mundo. Várias linhagens celulares, de células-tronco bem caracterizadas a frações contendo diferentes tipos de células, têm sido investigadas em modelos animais. Apesar dos avanços obtidos na última década, na área de ciência básica, com relação a esta nova modalidade terapêutica, diversas questões permanecem sem resposta. Pouco ainda se sabe sobre os mecanismos através dos quais a terapia celular possa gerar resultados efetivos. Adicionalmente, a melhor via para o transplante, o número total e a concentração de células, e o melhor tipo celular permanecem questões importantes, ainda sem definição. É fato de que diversas células da medula óssea exercem seus efeitos através de mecanismos parácrinos e de que existe um complexo mecanismo de interação, contato e liberação de sinais entre essas células e as outras populações celulares nos órgãos lesados. Atualmente, a maioria dos estudos em humanos se concentra em células de origem adulta e autóloga, em oposição ao uso de células de origem embrionária. Esta revisão analisa os principais ensaios clínicos que utilizaram células derivadas de medula óssea em quatro cardiopatias: doença arterial coronariana aguda e crônica, e nas cardiomiopatias chagásica e dilatada. Os resultados desses estudos demonstram que o procedimento é seguro e exequível, e potencialmente eficaz. Inquestionavelmente, mais estudos pré-clínicos e clínicos são necessários para acessar o real potencial benefício desse novo modelo terapêutico.Cell transplantation for the treatment of cardiovascular diseases is being investigated in many centers throughout the world. Various cell lines, from well characterized stem cells to cell fractions containing different types of cells, have been investigated in animal models. Despite progress in the basic research of

  10. Terapia nutricional no diabetes gestacional Nutritional therapy in gestational diabetes

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    Patricia de Carvalho Padilha

    2010-02-01

    Full Text Available Trata-se de uma revisão da literatura científica sobre a terapia nutricional no Diabetes Mellitus Gestacional, sem restrição de data e com fontes primárias indexadas nas bases de dados SciELO, PubMed, Medline. Os resultados desta revisão apontam a intervenção nutricional como uma importante aliada no controle do Diabetes Mellitus Gestacional, trazendo potenciais benefícios à saúde materno-fetal. Na avaliação do estado nutricional materno devem ser empregados os indicadores antropométricos, dietéticos, bioquímicos, clínicos e funcional. Neste sentido, a avaliação dietética deve ser detalhada, com atenção para o fracionamento e composição das refeições, e grupos de alimentos presentes. No planejamento nutricional a distribuição de macronutrientes em relação ao consumo energético diário deve ser 45-65% de carboidratos, 15-20% de proteínas e 20-35% de lipídeos. Quanto a recomendação dos edulcorantes, são liberados para gestantes acesulfame K, aspartame, neotame, sacarina e sucralose. A atividade física também deve fazer parte da estratégia de tratamento do Diabetes Mellitus Gestacional, embora o impacto do exercício nas complicações neonatais ainda mereça ser rigorosamente testado. Ademais, estudos associam a habilidade de aconselhamento nutricional com a melhorara na adesão ao cuidado nutricional. Diante desses achados, para sucesso no controle do DMG são necessários: a participação da equipe inter e multidisciplinar, o cuidado pré-natal precoce, com assistência nutricional oportuna e a garantia da assistência de qualidade ao longo da gestação.This is a scientific literature review about nutritional therapy in gestational diabetes mellitus, without date restriction and using the SciELO, PubMed and Medline databases. The results of this review show that nutritional intervention is an important tool for managing gestational diabetes mellitus, and potentially benefits the mother's and fetal health

  11. Resultados del seguimiento de pacientes con Trastorno Obsesivo Compulsivo tratados con dos variantes de Terapia Cognitiva Follow-Up of patients with Obsessive Compulsive Disorder treated with two variants of Cognitive Therapy

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    Ricardo Rodríguez Biglieri

    2007-12-01

    Full Text Available El artículo presenta los resultados del seguimiento a 6 meses realizado a pacientes con trastorno obsesivo compulsivo en el marco del proyecto UBACyT P079. Para el estudio se utilizó un diseño de caso único (A-B con replicación directa entre sujetos. Ocho pacientes fueron asignados aleatoriamente a una de las dos condiciones de tratamiento ofrecidas: 1 Terapia centrada en exposición y Prevención de respuesta; 2 Terapia Metacognitiva. Dos de los pacientes en la primera condición abandonaron el tratamiento tras la tercera sesión. Los cuatro de la segunda condición finalizaron el protocolo establecido. Los resultados para ambos tipos de terapia muestran una mejoría significativa en la sintomatología obsesiva y depresiva tras su aplicación, resultados que se han mantenido estables en las evaluaciones de seguimiento, sugiriendo la utilidad clínica de ambas terapias. Se discuten las implicaciones teóricas y prácticas de los resultados y se sugieren nuevas líneas de investigación.The article presents the follow-up at six months of patients with obsessive compulsive disorder treated in the context of UBACyT project P079. A single case design (A-B between subjects with direct replication was used. Eight patients were randomly assigned to one of two different treatment conditions: 1 Exposure and Response Prevention Therapy (Ex/PR; 2 Metacognitive Therapy (MT. Two patients in the Ex/PR condition dropped out after the third session. There was no dropout of patients under MT condition. Outcomes from both kinds of treatment show a significant improvement in obsessive and depressive sympthomatology after the interventions. These findings were sustained at the follow- up evaluations, suggesting that both treatments may be of clinical utility. Theoretical and practical implications are discussed, and further lines of investigation are outlined.

  12. O PACIENTE DE UNIDADE DE TERAPIA INTENSIVA E A PERCEPÇÃO DOS CUIDADOS DE HIGIENE CORPORAL

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    Cilene Aparecida Costardi Ide

    Full Text Available Neste estudo foram analisados aspectos relacionados à maneira como pacientes críticos percebem, vivenciam e avaliam o atendimento à necessidade de higiene corporal enquanto internados numa Unidade de Terapia Intensiva (UTI.

  13. Papel de D. Ángel Alonso Miguel en el desarrollo de la Terapia Ocupacional en España

    OpenAIRE

    Máximo Bocanegra, Nuria

    2010-01-01

    Tesis Doctotal leída en la Universidad Rey Juan Carlos en noviembre de 2010. Director de la Tesis: Dr. D. Juan Carlos Miangolarra Page Fisioterapia, Terapia Ocupacional, Rehabilitación y Medicina Física

  14. Uso dos equipamentos de proteção individual em unidade de terapia intensiva Uso de los equipamientos de protección individual en unidad de terapia intensiva Use of personal protective equipment in the intensive care unit

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    Ellen Lucy Vale de Souza

    2011-07-01

    Full Text Available Apesar dos grandes avanços na prevenção de infeções hospitalares, a adesão aos equipamentos de proteção individual continua sendo um importante desafio entre os trabalhadores da saúde. Foi objetivo deste estudo analisar a utilização de equipamentos de proteção individual (EPIs pelos trabalhadores de enfermagem nas unidades de terapia intensiva de um hospital de emergência localizado no município de Fortaleza/CE, Brasil. Trata-se de um estudo exploratório, descritivo e transversal, realizado nas quatro unidades da referida instituição. A coleta de dados ocorreu nos meses de setembro e outubro de 2008, através de entrevista e observação, respeitando-se os aspetos ético-legais. Participaram do estudo 45 trabalhadores da equipe de enfermagem da UTI, nas categorias de técnico e auxiliar. Os resultados revelam que os técnicos e auxiliares de enfermagem têm consciência da importância do uso de EPIs, porém não os utilizam com a devida frequência na prática de seu cotidiano laboral, devido a problemas de ordem comportamental e de logística. Os resultados deste estudo oferecem bases para uma reflexão quanto à importância da identificação de facilidades e dificuldades encontradas pela equipe na utilização dos EPIs.Apesar de los grandes avances en la prevención de infecciones hospitalarias, la adhesión a los equipamientos de protección individual sigue siendo un importante desafío entre los trabajadores de la salud. Este estudio tuvo por objetivo analizar la utilización de equipamientos de protección individual (EPIs por los trabajadores de enfermería en las unidades de terapia intensiva de un hospital de emergencia localizado en el municipio de Fortaleza/CE, Brasil. Se trata de un estudio exploratorio, descriptivo y transversal, realizado en las cuatro unidades de la referida institución. La recolección de datos transcurrió durante los meses de septiembre a octubre de 2008, mediante entrevista y observaci

  15. Obtención de la tenacidad de fractura dinámica de iniciación de materiales elastoplásticos a velocidades de deformación intermedias

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    Fernández-Sáez, José

    2001-06-01

    Full Text Available An earlier paper dealt with the experimental techniques used to determine the dynamic fracture properties of linear elastic materials. Here we describe those most commonly used as elastoplastic materials, limiting the study to the initiation fracture toughness at the intermediate strain rate (of around 10 s2 s-1 . In this case the inertial forces are negligible and it is possible to apply the static solutions. With this stipulation, the analysis can be based on the methods of testing in static conditions. The dynamic case differs basically, from the static one, in the influence of the strain rate on the properties of the material.

    En un trabajo previo se han expuesto las técnicas experimentales aplicables a la determinación de las propiedades de fractura en condiciones dinámicas de materiales elásticos y lineales. En este trabajo se describen las más utilizadas para la determinación de las propiedades de fractura en condiciones dinámicas de materiales elastoplásticos. El estudio se restringe a la determinación de la tenacidad de fractura dinámica de iniciación a velocidades de deformación intermedias (del orden de 102 s-1, caso en el que las condiciones son tales que las fuerzas de inercia son despreciables y, por tanto, son aplicables las soluciones estáticas. Esta premisa permite utilizar como base de análisis la metodología de los ensayos en condiciones estáticas. La diferencia fundamental con el caso estático estriba en la influencia de la velocidad de deformación en las propiedades del material.

  16. Carbon monoxide-releasing molecule-3 suppresses Prevotella intermedia lipopolysaccharide-induced production of nitric oxide and interleukin-1β in murine macrophages.

    Science.gov (United States)

    Choi, Eun-Young; Choe, So-Hui; Hyeon, Jin-Yi; Choi, Jeom-Il; Choi, In Soon; Kim, Sung-Jo

    2015-10-05

    This study was performed to analyze the effect of carbon monoxide (CO)-releasing molecule-3 (CORM-3) in alleviating the production of proinflammatory mediators in macrophages treated with lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen associated with periodontal disease, and its possible mechanisms of action. LPS was isolated using the hot phenol-water method. Culture supernatants were assayed for nitric oxide (NO) and interleukin-1β (IL-1β). Gene expression was quantified by real-time PCR, and protein expression by immunoblotting. DNA-binding activities of NF-κB subunits were determined using an ELISA-based kit. CORM-3 suppressed the production of inducible NO synthase (iNOS)-derived NO and IL-1β at both gene transcription and translation levels in P. intermedia LPS-activated RAW264.7 cells. CORM-3 enhanced heme oxygenase-1 (HO-1) expression in cells stimulated with P. intermedia LPS, and inhibition of HO-1 activity by SnPP notably reversed the suppressive effect of CORM-3 on LPS-induced production of NO. LPS-induced phosphorylation of p38 and JNK was not affected by CORM-3. CORM-3 did not influence P. intermedia LPS-induced degradation of IκB-α. Instead, nuclear translocation of NF-κB p65 and p50 subunits was blocked by CORM-3 in LPS-treated cells. In addition, CORM-3 reduced LPS-induced p65 and p50 binding to DNA. Besides, CORM-3 significantly suppressed P. intermedia LPS-induced phosphorylation of STAT1. Overall, this study indicates that CORM-3 suppresses the production of NO and IL-1β in P. intermedia LPS-activated murine macrophages via HO-1 induction and inhibition of NF-κB and STAT1 pathways. The modulation of host inflammatory response by CORM-3 would be an attractive therapeutic approach to attenuate the progression of periodontal disease. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. Terapia comunitária: relato de uma experiência

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    Ângela Cristina Nogueira

    2017-03-01

    Full Text Available No presente trabalho apresentamos uma experiência de Terapia Comunitária Integrativa, realizada em classes do ensino médio, no Colégio Modelo Luís Eduardo Magalhães, situado em um bairro de periferia da cidade de Salvador, pertencente à rede pública do Estado da Bahia. O objetivo principal do trabalho consistiu em acolher pessoas da comunidade escolar (alunos, pais, mães, avós e/ou responsáveis e da administração do Colégio (direção, coordenadores, professores e funcionários, na tentativa de promover mudanças nas atitudes dessas Instituições, de pessoas outras ligadas ao processo de educação, dos alunos e familiares envolvidos, da comunidade a que pertencem e, consequentemente, da Sociedade como um todo. A metodologia utilizada consistiu, primeiramente em criar espaço necessário, dentro do horário escolar em paralelo com a atividade curricular normal onde foram realizadas rodas de terapia, visando o desenvolvimento do aluno como ser humano e como cidadão. Essas rodas de terapia, cuja característica é ser trabalho grupal aberto, foram desenvolvidas no período de Maio/2010 a Junho/2011, com alunos da primeira, segunda e terceira séries, e com professores dessas classes, nos turnos matutino e vespertino. Depoimentos e questionários aplicados, nesse período, nas rodas de terapia comunitária nos deram subsídios para dados estatísticos que anexaremos ao trabalho. Embora os resultados ainda não tenham sido aferidos no aspecto quantitativo, os depoimentos de professores e alunos e responsáveis atestam o êxito obtido nos resultados das rodas de terapia comunitária, ali realizadas, o que nos leva a concluir que vale a pena continuar investindo e lutando pela inclusão da Terapia Comunitária na Educação.

  18. Bacterial Resistance and Mortality in an Intensive Care Unit Resistencia bacteriana y mortalidad en un Centro de Terapia Intensiva Resistência bacteriana e mortalidade em um centro de terapia intensiva

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    Adriana Cristina de Oliveira

    2010-12-01

    Full Text Available The goal was to identify risk factors for healthcare-associated infections by resistant microorganisms and patient mortality in an Intensive Care Unit. A prospective and descriptive epidemiological research was conducted from 2005 till 2008, involving 2300 patients. Descriptive statistics, bivariate and multivariate logistic regression analysis were used. In bivariate analysis, infection caused by resistant microorganism was significantly associated to patients with community-acquired infection (p=0.03; OR=1.79 and colonization by resistant microorganism (pSe objetivó identificar factores de riesgo para el desarrollo de infecciones relacionadas al cuidar en salud, por microorganismos resistentes, y también investigar su relación con la mortalidad de los pacientes en un centro de terapia intensiva. Se trata de un estudio epidemiológico prospectivo realizado entre 2005 y 2008, envolviendo 2.300 pacientes. Se utilizó la estadística descriptiva y el análisis de regresión logístico bivariado y multivariado. En el análisis bivariado, la infección por microorganismos resistentes estuvo significativamente asociada a pacientes con infección comunitaria (p=0,03; OR=1,79 y a la colonización por microorganismo resistente (pObjetivou-se identificar fatores de risco para o desenvolvimento de infecções, relacionadas ao cuidar em saúde, por microrganismos resistentes e a mortalidade dos pacientes em um centro de terapia intensiva. Trata-se de estudo epidemiológico prospectivo, realizado entre 2005 e 2008, envolvendo 2.300 pacientes. Utilizou-se estatística descritiva, análise de regressão logística bivariada e multivariada. Na análise bivariada, a infecção por microrganismo resistente esteve significativamente associada a pacientes com infecção comunitária (p=0,03; OR=1,79 e colonização por microrganismo resistente (p<0,01; OR=14,22. Na análise multivariada, severidade clínica C (p=0,03; OR=0,25 e colonização por microrganismo

  19. Fatores associados à carga de trabalho de enfermagem em Unidade de Terapia Intensiva Factores asociados a la carga de trabajo de enfermería en Unidad de Terapia Intensiva Factors associated with nursing workload in adult intensive care units

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    Leilane Andrade Gonçalves

    2007-12-01

    Full Text Available Os objetivos desta pesquisa foram analisar a carga de trabalho de enfermagem e os fatores associados a ela, no primeiro dia de internação dos pacientes na Unidade de Terapia Intensiva (UTI. Trata-se de um estudo retrospectivo, de corte transversal e com abordagem quantitativa, realizado em abril de 2002 e outubro de 2004. Os dados foram extraídos de um banco de dados que reuniu informações de 5 UTIs de dois hospitais privados e a amostra foi constituída por 214 pacientes adultos que permaneceram no mínimo 24 horas na UTI. A média do escore total do Nursing Activities Score (NAS foi de 69,9% e mediana de 68,0%. Verificou-se, segundo a mediana, que 109 (50,9% indivíduos tiveram alta carga de trabalho de enfermagem e 105 (49,1% baixa carga. Observou-se também que a gravidade, a idade do paciente e o tipo de tratamento não foram fatores associados à demanda de trabalho de enfermagem.Los objetivos de esta pesquisa fueron analizar la carga de trabajo de enfermería y los factores asociados a ella, en el primer día de internación de los pacientes en la Unidad de Terapia Intensiva (UTI. Se trata de un estudio retrospectivo, de corte transversal y con abordaje cuantitativa, realizado en abril de 2002 y octubre de 2004. Los datos fueron extraídos de un banco de datos que reunió informaciones de 5 UTIs de dos hospitales particulares y la muestra fue constituida por 214 pacientes adultos que permanecieron en el mínimo de 24 horas en la UTI. La media de escore total del Nursing Activities Store (NAS fue de 69,9% y mediana de 68,0%. Se verificó, según la mediana, que 109 (50,9% individuos tuvieron alta carga de trabajo de enfermería y 105 (49,1% baja carga. Se observó también que la gravedad, la edad del paciente y el tipo de tratamiento no fueron factores asociados a la demanda de trabajo de enfermería.This study was aimed both at analyzing the nursing workload on the first day of admission of patients in Intensive Care Units (ICUs and

  20. Qualidade de vida de pacientes com câncer de mama em terapia adjuvante Calidad de vida de pacientes con cáncer de mama en terapia adyuvante Quality of life of breast cancer patients in adjuvant therapy

    Directory of Open Access Journals (Sweden)

    Adriana Cristina Nicolussi

    2011-12-01

    Full Text Available O objetivo do estudo foi avaliar a Qualidade de Vida (QV, identificar os domínios afetados em pacientes com câncer de mama em terapia adjuvante e correlacioná-los com características sócio-demográficas, clínicas e terapêuticas. Estudo transversal, realizado com 35 pacientes entre agosto de 2007 e dezembro de 2008. Utilizou-se o instrumento Quality of Life Core-30-Questionnaire (QLQ-C30. A Qualidade de Vida geral foi considerada pouco satisfatória. Os domínios afetados foram: função emocional, insônia, dor e fadiga. Nas correlações, as pacientes acima de 60 anos apresentaram pior escore na função emocional, as submetidas à cirurgia referiram mais constipação, as que realizavam radioterapia obtiveram pior escore para QV geral e as que estavam em quimioterapia há mais de seis ciclos apresentaram a função emocional afetada e dispnéia. Concluiu-se que os tratamentos adjuvantes afetaram de algum modo as pacientes, causando déficit na função emocional e relataram mais sintomas, prejudicando assim sua QV.El objetivo fue evaluar la calidad de vida (CV, identificar los dominios afectados en pacientes con cáncer de mama en terapia adyuvante y correlacionarlos con las características socio-demográficas, clínicas y terapéuticas. Estudio transversal, con 35 pacientes, realizado entre agosto de 2007 y diciembre de 2008. Fue utilizado el instrumento Quality of Life Core-30-Questionnaire (QLQ-C30. La calidad de vida fue considerada poco satisfactoria. Los dominios más afectados fueron: función emocional, insomnio, dolor, fatiga. En las correlaciones, las pacientes con más de 60 años presentaron peores promedios en la función emocional, las pacientes que fueron sometidas a la cirugía refirieron más estreñimiento, las que recibían radioterapia presentaron peores promedios en CV general y las que habían recibido más de seis ciclos de quimioterapia presentaron la función emocional afectada y la disnea. Se concluyó que los

  1. Dispersal and survival of Nyssomyia intermedia and Nyssomyia neivai (Diptera: Psychodidae: Phlebotominae in a cutaneous leishmaniasis endemic area of the speleological province of the Ribeira Valley, state of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Eunice AB Galati

    2009-12-01

    Full Text Available The dispersal and survival of the phlebotomines Nyssomyia intermedia and Nyssomyia neivai (both implicated as vectors of the cutaneous leishmaniasis agent in an endemic area was investigated using a capture-mark-release technique in five experiments from August-December 2003 in municipality of Iporanga, state of São Paulo, Brazil. A total of 1,749 males and 1,262 females of Ny. intermedia and 915 males and 411 females of Ny. neivai were marked and released during the five experiments. Recapture attempts were made using automatic light traps, aspiration in natural resting places and domestic animal shelters and Shannon traps. A total of 153 specimens (3.48% were recaptured: 2.59% (78/3,011 for Ny. intermedia and 5.35% (71/1,326 for Ny. neivai. Both species were recaptured up to 144 h post-release, with the larger part of them recaptured within 48 h. The median dispersion distances for Ny. intermedia and Ny. neivai, respectively, were 109 m and 100 m. The greatest dispersal range of Ny. intermedia was 180 m, while for Ny. neivai one female was recaptured in a pasture at 250 m and another in a pigsty at 520 m, showing a tendency to disperse to more open areas. The daily survival rates calculated based on regressions of the numbers of marked insects recaptured on the six successive days after release were 0.746 for males and 0.575 for females of Ny. intermedia and 0.649 for both sexes of Ny. neivai. The size of the populations in the five months ranged from 8,332-725,085 for Ny. intermedia males, 2,193-104,490 for Ny. intermedia females, 1,687-350,122 for Ny. neivai males and 254-49,705 for Ny. neivai females.

  2. Foto personali e foto di famiglia come strumento per la terapia. Il “Come, Cosa e Perché” delle tecniche di FotoTerapia

    Directory of Open Access Journals (Sweden)

    Judy Weiser

    2010-12-01

    Full Text Available L’articolo spiega come e perché, l’utilizzo delle foto personali e quelle di famiglia delle persone in terapia durante il loro trattamento (“Tecniche di FotoTerapia” possa aumentare e approfondire tale processo, e migliorare la qualità dei risultati terapeutici. Si descrive come le foto creino un ponte in grado di raggiungere aree profonde del vissuto del cliente dove le parole non possono arrivare – fornendo quindi un eccellente mezzo per esaminare ed esplorare i sentimenti con il minimo dell’interferenza e delle difese cognitive. Basato sul principio che le fotografie presentano accanto ai dettagli visivi un contenuto emotivo, questo articolo mostra come il terapeuta formato nelle Tecniche di FotoTerapia possa usare le foto personali e quelle di famiglia del cliente per evocare ricordi, richiamare sentimenti, riconnettere pensieri, nascondere segreti, e portare alla luce il passato con nuove modalità. Si mostra come le Tecniche di FotoTerapia siano basate sul fatto che tutte le fotografie parlano il linguaggio metaforico e simbolico dell’inconscio, senza usare le parole, e quindi ogni immagine fotografica è in grado, indipendentemente dalla modalità preferita dal terapeuta, di raggiungere informazioni e sentimenti precedentemente bloccati o fortemente difesi. Dopo l’” Introduzione” e le Sezioni relative alle “Arti (e Arte Terapia” e “Fotografie (e Fotografia” che propongono le basi teoriche delle tecniche di FotoTerapia, l’articolo procede da una rassegna generale dell’“Uso delle Fotografie nella Pratica Terapeutica” a una spiegazione più dettagliata della “Cornice di Lavoro delle Tecniche di FotoTerapia”, discutendo i principi e le specifiche applicazioni di ogni tecnica, da usare singolarmente o in combinazione con le altre. È inoltre inclusa una comparazione tra le tecniche di “FotoTerapia” e le analoghe applicazioni sia della “Foto Arte Terapia” che della “Fotografia Terapeutica

  3. técnica de relajación vs fármaco de síntesis en el tratamiento de la ansiedad del paciente oncológico: estudio del metabolismo cerebral mediante Pet con 18f-fluorodesoxiglucosa (fdg)

    OpenAIRE

    Pifarré Montaner, Paloma

    2014-01-01

    Existe un crecimiento progresivo de la utilización de terapias naturales (TN) en occidente y especialmente en los pacientes oncológicos. El cáncer es una de los problemas de salud fundalmentales de nuestra sociedad. Cuando existe una sospecha o diagnóstico de cáncer, los estudios de diagnóstico por la imagen tienen un papel fundalmental en la actividad clínica de este grupo de pacientes. Estos estudios pueden conllevar un grado importante de ansiedad. La tesis tiene dos objetivos fundamentale...

  4. Terapia comportamental construtiva: uma outra face da clínica comportamental

    Directory of Open Access Journals (Sweden)

    Luc Vandenberghe

    2007-12-01

    Full Text Available Este artigo apresenta a terapia comportamental construtiva conforme elaborada por Beata Bakker-de Pree. A abordagem, desenvolvida nos Países Baixos, traz uma filosofia do processo terapêutico que é compatível com a de várias terapias comportamentais conhecidas. Acrescenta uma perspectiva valiosa em sua descrição explícita de alguns ingredientes importantes do trabalho clínico, que não estão em destaque nos outros modelos comportamentais, sendo estas a técnica de diferenciação livre e a análise funcional do comportamento saudável do cliente.

  5. La estructura de la pareja: implicaciones para la terapia cognitivo conductual

    Directory of Open Access Journals (Sweden)

    JOSÉ ANTONIO GARCÍA HIGUERA

    2002-01-01

    Full Text Available La terapia de pareja cognitivo conductual ha mostrado su eficacia de manera empírica (Chambless et al., 1998. Sin embargo, existen limitaciones (Christensen y Heavey 1999 que indican la necesidad de su potenciación. Este artículo repasa la situación de la terapia de pareja, con las aportaciones actuales, enmarcando todo ello en una visión estructural de la pareja, como ente social y relación diádica, que permita una compresión de los avances que se están dando, y aporte indicaciones sobre los caminos que seguirá en un futuro inmediato.

  6. Modelos animales de degeneración cognitiva y uso de transplantes neurales de células madre como terapia

    Directory of Open Access Journals (Sweden)

    Alcyr Alves de Oliveira

    2017-02-01

    Full Text Available Existen pocos modelos animales de degeneración cognitiva que podrían ser considerados tan importantes como el modelo de lesión colinérgica de la enfermedad de Alzheimer. La enfermedad de Alzheimer es una patología neurodegenerativa asociada con el envejecimiento y con degeneración cognitiva acentuada y progresiva. Sus complejas y múltiples vías neurales secuenciales pueden conducir a la comprensión de que las intervenciones de agente único pueden ser insuficiente para impactar el curso de esta enfermedad. El tratamiento farmacológico actual es paliativo y, aunque pueda retardar el progreso de la enfermedad, no es capaz de ofrecer una cura. La combinación de drogas y sustancias antioxidantes han sido investigadas, sin embargo, existen pocos estudios que demuestran resultados consistentes basados en esta presunción. Así, el objetivo de este artículo fue destacar las terapias basadas en la reposición celular como potencial tratamiento para enfermedades del sistema nervioso periférico y central.

  7. Terapia familiar sistémica: una aproximación a la teoría y la práctica clínica

    Directory of Open Access Journals (Sweden)

    David Villarreal-Zegarra

    2016-06-01

    Full Text Available RESUMEN: El presente estudio teórico, tiene como objetivo proporcionar un acercamiento conceptual y técnico a la terapia familiar sistémica (TFS. El trabajo inicia señalando los postulados de la teoría general de los sistemas, la cibernética de segundo orden, la teoría de la comunicación humana orientados a la representación práctica, la concepción de la familia, su ciclo evolutivo, las etapas de crisis que vivencian y como se llega a formar un sistema terapéutico. Se concluye que el enfoque es posee evidencia empírica, que sostiene, y en la actualidad la TFS se está transformando, abandonando y/o reinventando algunos de sus postulados originales por nuevos constructos. ABSTRACT: This theoretical study aims to provide a conceptual and technical approach to systemic family therapy (SFT. The work starts pointing the postulates of general systems theory, cybernetics second-order theory of human communication practice-oriented representation, the conception of the family, their evolutionary cycle stages of crisis vivencian as you get to form a therapeutic system. It is concluded that the approach is empirical evidence has, holding, and today the SFT is becoming abandoning and / or reinventing some of its original tenets of new constructs.

  8. La Terapia de Aceptación y Compromiso (ACT en el consumo de sustancias como estrategia de Evitación Experiencial

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    Carmen Luciano

    2010-01-01

    Full Text Available Numerosos estudios han corroborado que la regulación de los eventos privados focalizada en la evitación experiencial juega un papel fundamental, tanto en el desarrollo de las adicciones como en su mantenimiento, llegando a producir el patrón destructivo que caracteriza a las personas ancladas en el consumo desajustado de sustancias. Las contingencias a la base del comportamiento adictivo constituyen el Trastorno de Evitación Experiencial (TEE que implica una estrategia inflexible, formada por un conjunto de acciones dirigidas al escape y la evitación de malestar, y sustentada por la coherencia ¿establecida socialmente- que supone la necesidad de regular el malestar de modo inmediato. La Terapia de Aceptación y Compromiso (ACT está diseñada para el abordaje de la Evitación Experiencial Destructiva (el TEE. En el contexto del comportamiento adictivo, la ACT ha mostrado ser eficaz en el desarrollo de una vida consistente con los valores personales cuyo coste principal supone afrontar el malestar que se deriva de la abstinencia y otras sensaciones, pensamientos y recuerdos que pudieran ser dolorosos. El presente artículo tiene como objetivo la exposición ejemplificada de los componentes fundamentales de ACT en el caso de personas con problemas de adicciones a diferentes sustancias.

  9. Una reflexión sobre la Psicología Positiva y la Terapia de Aceptación y Compromiso

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    Carmen Luciano

    2006-01-01

    Full Text Available La Psicología Positiva aboga por el estudio científico de la felicidad, el análisis de las condiciones que la hacen posible y la búsqueda de indicadores objetivos ligados tanto al bienestar de los ciudadanos en general como a la promoción de características personales que se relacionan con este bienestar y que comprenden un acopio de experiencias subjetivas calificadas de positivas. La Terapia de Aceptación y Compromiso analiza los denominados trastornos psicológicos desde una perspectiva funcional, centrada en la evidencia experimental sobre las características del hecho de ser verbales y lo que ello trae consigo en el ámbito cultural de referencia. En este breve artículo se presentan los puntos de encuentro, algunas de las divergencias y, especialmente, las aportaciones que diluciden métodos preventivos y clínicos para que las personas podamos afrontar la vida en armonía con las características de la condición humana.

  10. INFECÇÃO URINÁRIA EM UNIDADE DE TERAPIA INTENSIVA: UM INDICADOR DE PROCESSO PARA PREVENÇÃO

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    Mayra Gonçalves Menegueti

    2012-01-01

    Full Text Available Este estudio descriptivo con objetivo de evaluar los factores predisponentes para ocurrencia de Infección del Tracto Urinario en pacientes críticamente enfermos en unidad de terapia intensiva. Se utilizó un indicador de evaluación de las condiciones de manutención del cateterismo vesical de demora, constituido de cinco componentes. La recolección de datos ocurrió entre julio y agosto de 2007, diariamente, por medio de observaciones directas. De 471 observaciones, el componente posicionamiento de la bolsa de recogida obtuvo 100% de adecuación, sistema cerrado de drenaje 99%, flujo de orina sin obstáculos 96%, volumen de orina abajo de dos tercios del nivel de la bolsa de recogida 96% y fijación de la sonda vesical fue atendida en 7%, comprometiendo el indicador evaluado. Los indicadores de proceso son herramientas útiles para la prevención del control de infección, pues permiten evaluaciones sistemáticas de las intervenciones y, consecuentemente, proposiciones de estrategias educativas mejor estructuradas.

  11. Respostas fisiológicas e comportamentais de recém-nascidos durante o manuseio em unidade de terapia intensiva neonatal

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    Fernanda Jorge Magalhães

    2011-01-01

    Full Text Available En la Unidad de Terapia Intensiva Neonatal (UTIN, el recién nacido (RN recibe tratamiento de urgencia para mejorar su condición clínica, con varios episodios de manejo. El objetivo fue verificar las respuestas fisiológicas y de comportamiento del recién nacido hospitalizado en la UTIN y proponer un protocolo de manejo mínimo del RN en la UTIN. Estudio exploratorio, descriptivo, planteo cuantitativo; realizado en la UTIN de un hospital universitario, en Fortaleza/ Ceará. Muestra de 26 RNs; los datos se recogieron en abril de 2010. Se comprobó que los recién nacidos presentaron un promedio de ocho respuestas fisiológicas o de comportamiento. Las principales fueron: arquear las cejas, respuestas faciales y aumento del tono muscular, lo que puede suponer para el recién nacido malestar, desorganización y dolor ante los procedimientos. Se concluye que el manejo excesivo altera los aspectos fisiológicos y de comportamiento del RN, de modo que el protocolo de manejo mínimo del RN puede ser una estrategia para mantener el bienestar.

  12. Ideación suicida y respuesta a la terapia cognitiva conductual en adolescentes puertorriqueños/as con depresión mayor

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    Jeannette Rosselló

    2011-01-01

    Full Text Available En Puerto Rico existe una alta prevalencia de depresión e ideación suicida (IS en adolescentes. Este estudio examinó la respuesta a una terapia cognitivo-conductual para la depresión con un protocolo para el manejo del riesgo suicida incorporado. Participaron 115 adolescentes con depresión mayor. Se realizaron análisis descriptivos, pruebas t de medidas repetidas, análisis de significancia clínica e índice de cambio confiable, correlaciones y de regresión múltiple. La IS al comienzo del tratamiento no tuvo un efecto sobre el tratamiento para la depresión. Un 34.8% de los/as participantes demostraron un índice de cambio confiable de las puntuaciones del pre al post tratamiento en IS y un 89.6% evidenciaron cambio clínico significativo en post-tratamiento. El nivel de desesperanza y la IS al comienzo del tratamiento mostraron un valor predictivo hacia el nivel de IS al finalizar el tratamiento. En conclusión, la mayoría de los/as adolescentes con depresión e IS tratados con la TCC redujeron significativamente sus pensamientos suicidas.

  13. Full structure and insight into the gene cluster of the O-specific polysaccharide of Yersinia intermedia H9-36/83 (O:17).

    Science.gov (United States)

    Sizova, Olga V; Shashkov, Alexander S; Kondakova, Anna N; Knirel, Yuriy A; Shaikhutdinova, Rima Z; Ivanov, Sergei A; Kislichkina, Angelina A; Kadnikova, Lidia A; Bogun, Aleksandr G; Dentovskaya, Svetlana V

    2018-05-02

    Lipopolysaccharide was isolated from bacteria Yersinia intermedia H9-36/83 (O:17) and degraded with mild acid to give an O-specific polysaccharide, which was isolated by GPC on Sephadex G-50 and studied by sugar analysis and 1D and 2D NMR spectroscopy. The polysaccharide was found to contain 3-deoxy-3-[(R)-3-hydroxybutanoylamino]-d-fucose (d-Fuc3NR3Hb) and the following structure of the heptasaccharide repeating unit was established: The structure established is consistent with the gene content of the O-antigen gene cluster. The O-polysaccharide structure and gene cluster of Y. intermedia are related to those of Hafnia alvei 1211 and Escherichia coli O:103. Copyright © 2018 Elsevier Ltd. All rights reserved.

  14. Postembryonic development of Loxosceles intermedia Mello-Leitão, 1934, L. laeta (Nicolet, 1849 and L. gaucho Gertsch, 1967 (Araneae; Sicariidae breeding under conditions of monospecific diet

    Directory of Open Access Journals (Sweden)

    Emanuel Marques da Silva

    2005-05-01

    Full Text Available The influence of monospecific feeding on the post-embryonic development of L. intermedia, L. laeta and L. gaucho was evaluated. Two hundred and ten spiderlings were individualized in containers (120 ml, 105 being fed with Tenebrio molitor larvae and 105 with Pycnoscelus surinamensis nymphs. In the three species, the number of molts varied according to the diet. The number of spiders that reached maturity was lower in L. gaucho. In L. intermedia, the duration of the post-embryonic period was greater when larvae are fed. Mortality was higher in the second instar in the three species, the highest frequency being registered for L. gaucho. The data provided evidence that monospecific feeding influenced the post-embryonic development of the studied species. This influence might intensified by specific characteristics such as origin, habits and habitats.

  15. Indução de calos em explantes foliares de Murici-pequeno (Byrsonima intermedia A. Juss. Calli induction from leaf explants of murici-pequeno (Byrsonima intermedia A. Juss.

    Directory of Open Access Journals (Sweden)

    Raírys Cravo Nogueira

    2007-04-01

    Full Text Available O murici-pequeno (Byrsonima intermedia A. Juss. é um arbusto do cerrado cujo chá da casca do caule apresenta atividade adstringente nas diarréias e disenterias. O gênero Byrsonima apresenta taxa de germinação baixa e emergência lenta da plântula, dificultando a propagação sexuada. Objetivou-se com este trabalho obter calos friáveis em explantes foliares de murici-pequeno para estudos futuros em suspensão celular e metabolismo secundário, assim como embriogênese somática. Avaliou-se o efeito de diferentes concentrações da auxina 2,4-D e a sua interação com as citocininas TDZ e BAP na calogênese. Os resultados demonstraram que na ausência de 2,4-D não ocorre formação de calo em explantes foliares. A adição de BAP ou TDZ não influencia o processo de calogênese. Para a indução e proliferação de calo em explantes foliares de murici-pequeno, os resultados recomendam o uso de meio MS, acrescido de 1,0 mg L-1 de 2,4-D, mantendo os explantes em condição de escuro por 30 dias.Byrsonima intermedia A. Juss is a shrub of cerrado whose husk presents medicinal activities in diarrheas and dysenteries. The Byrsonima genus present low germination rate and slow plantlet emergency which makes difficult sexual propagation. The objective of this work was to obtain friable callus in leaf explants for future studies with suspension cells and secondary metabolites as well as somatic embryogenesis. The effect of different concentrations of 2,4-D and its interaction with TDZ and BAP in callus formation was evaluated. The results demonstrated that there is no formation of callus in leaf explants maintained in absence of 2,4-D. The addition of TDZ or BAP had no influence in the calogenesis process. For callus induction and proliferation, the results suggest the use of MS medium supplemented with 1.0 mg L-1 2,4-D maintaining the explants in the dark.

  16. Terapia EMDR en el trastorno límite de personalidad

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    Dolores Mosquera

    2013-06-01

    Full Text Available Los trastornos de personalidad se han asociado a problemas de apego y trauma temprano, siendo de todos ellos el trastorno límite el que presenta unos porcentajes más elevados. La aplicación de terapias orientadas al trauma como EMDR (Desensibilización y Reprocesamiento por Movimientos Oculares en estos trastornos ofrece interesantes posibilidades para su abordaje.

  17. Habilidades Clínicas en la Terapia Conductual Tercera Generación

    Directory of Open Access Journals (Sweden)

    Jorge Barraca Mairal

    2009-01-01

    Full Text Available En este artículo se repasan las técnicas de tres de las terapias de tercera generación conductual (Terapia de Aceptación y Compromiso, Psicoterapia Analítico Funcional y Terapia Conductual Dialéctica para reflexionar sobre el tipo de destrezas que el terapeuta debe desarrollar si pretende lograr una actuación competente al practicarlas. Este examen lleva a distinguir entre: (1 habilidades de escucha y discriminación de respuesta; (2 habilidades de fluidez, repertorio y competencia verbal; (3 habilidades de ejecución motora; (4 habilidades de imaginación-creatividad; (5 habilidades de conexión-empatía; y (6 otras habilidades (trabajo en grupo entre terapeutas, meditación, atención plena, etc.. Un análisis de estas destrezas permite concluir que los nuevos desarrollos parten de las tradicionales habilidades del terapeuta cognitivo-conductual pero replanteadas y con formas decididamente más elaboradas. Por otro lado, se revela que las competencias habitualmente entrenadas en la licenciatura, así como las que pueden derivarse exclusivamente de la lectura de los textos en que se divulgan estas terapias, resultan limitadas para un trabajo eficaz dentro de estos nuevos modelos. En conclusión, se propone la implementación de una formación práctica o procedimental a través de talleres, materiales audio-visuales y supervisión de casos.

  18. Terapia cognitivo-comportamental da depressão Cognitive-behavioral therapy for depression

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    Vania Bitencourt Powell

    2008-10-01

    Full Text Available OBJETIVO: Descrever o uso de técnicas cognitivas e revisar os estudos de eficácia da terapia cognitivo-comportamental no tratamento da depressão. MÉTODO: Revisão não-sistemática proveniente dos estudos originais, complementada por informações provenientes de metanálises e livros-texto especializados. RESULTADOS: Foram descritos os fundamentos da terapia cognitivo-comportamental no tratamento da depressão e revisadas as evidências de eficácia em curto e longo prazo. Discutimos igualmente o uso de tratamento farmacológico concomitante à terapia cognitivo-comportamental. CONCLUSÕES: A terapia cognitivo-comportamental é uma das abordagens que apresentam mais evidências empíricas de eficácia no tratamento da depressão, quer oferecida de forma isolada ou em combinação com farmacoterapia.OBJECTIVE: To describe the use of cognitive techniques and to review studies on the efficacy of CBT in the treatment of depression. METHOD: A non-systematic review of the literature of original studies complemented with data from meta-analyses and specialized textbooks. RESULTS: The fundamentals of cognitive-behavioral therapy in the treatment of depression are described and the evidence of short- and long-term efficacy is reviewed. The use of pharmacological therapy in conjunction with CBT is also discussed. CONCLUSIONS: CBT in the treatment of depression is one of the therapeutic modalities with the highest empirical evidence of efficacy, whether applied alone or in combination with pharmacotherapy.

  19. La terapia di Niccolò: la macchinetta con la panciolla

    Directory of Open Access Journals (Sweden)

    Fabiana Fossati

    2009-12-01

    Full Text Available In questo articolo dal taglio preminentemente clinico, l’autrice ci fa entrare nella stanza d’analisi conducendoci attraverso la terapia di un bambino di quattro anni e mezzo che presenta una diagnosi di “Disturbo Generalizzato dello Sviluppo”. Fin da subito è evidente l’impostazione dialogica fra materiale clinico e riflessioni personali dell’analista, impostazione che ha il grande pregio di permettere al lettore di seguire in vivo il progresso del  lavoro  terapeutico,  attraverso  una  finestra  sulla  terapia  e  sui  processi  mentali dell’analista stesso.Il processo terapeutico si dispiega in questo modo attraverso il racconto dei processi di cambiamento del mondo esterno e interno del bambino, che nel corso della terapia compie quasi una seconda nascita, un dischiudersi all’esterno attraverso un processo di strutturazione del Sé.Di grande interesse risulta inoltre il rapporto con i genitori del bambino, che attraverso la terapia del proprio figlio, e un contatto diretto con la terapeuta per mezzo di sedute a frequenza quindicinale, operano loro stessi un processo di cambiamento, interiorizzando una funzione di rispecchiamento e significazione dei vissuti propri e del bambino, grazie all’identificazione con la terapeuta stessa. In tal senso, oltre che una pregevole narrazione clinica, questo lavoro presenta degli spunti di enorme interesse per quanto riguarda il lavoro con i genitori nelle terapie in età evolutiva, toccando importanti tematiche da un punto di vista clinico, che possono aprire delle utili riflessioni sulla genitorialità in generale.

  20. Tratamento cirúrgico e terapias adjuvantes na papilomatose respiratória

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    Melissa Ameloti Gomes Avelino

    2013-10-01

    Full Text Available Papilomatose respiratória recorrente ou papilomatose laríngea recorrente é uma doença da laringe, causada pelo papiloma vírus humano, caracterizada por lesões epiteliais verrucosas e, geralmente, recorrentes. Na literatura são descritos diversos tipos de tratamento, como cirurgia a frio, a laser e/ou uso de microdebridador, além das terapias adjuvantes; todas no sentindo de diminuir possíveis sequelas permanentes da doença. OBJETIVO: Realizar uma revisão de literatura a respeito desta doença com ênfase nas técnicas cirúrgicas e terapias adjuvantes mais utilizadas atualmente. MÉTODO: Utilizou-se a metodologia de revisão bibliográfica, por meio de levantamentos em base de dados eletrônicos de domínio público, entre 1992-2012, utilizando-se as palavras-chave: papiloma, infecções por papillomavírus, laringe, terapêutica, vacinas contra papillomavírus. RESULTADOS: Foram levantados 357 artigos, dos quais 49 foram usados como base para esta revisão. Os trabalhos científicos apontam para a redução de recidiva na maioria das terapêuticas adjuvantes. Entretanto, o levantamento demonstrou metodologias e amostras diferentes, o que não permitiu comparar os tipos de tratamento e de terapias adjuvantes. CONCLUSÃO: A escolha da técnica cirúrgica varia entre os autores, porém, há uma tendência atual ao uso do microdebridador. As terapias adjuvantes recentes, como cidofovir, vacina tetravalente contra o papiloma vírus humano e bevacizumab, necessitam de estudos mais amplos.