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Sample records for tallinn estonian ssr

  1. Russification of non-Estonian pupils in Tallinn

    Directory of Open Access Journals (Sweden)

    Ülle Rannut

    2010-05-01

    Full Text Available In the article home languages, their qualities and use by pupils in grades 2 to 5 in Tallinn are analyzed. Two larger and more characteristic groups (Ukrainians and Belarusians and two groups with Islamic backgrounds (Azerbaijanis and Tatars were compared with the aim of analysing different language loss and preservation factors. Although Ukrainians and Belarusians form much larger ethnic groups within Estonia, they have not managed to preserve their language anywhere near as successfully as the Azerbaijanis and Tatars. Comparison of the data suggests that language preservation in Estonia and the ability to withstand Russification are most influenced by the degree of difference of the language from Russian and the religious difference from the Russian Orthodox faith (and Soviet era atheism. Home languages are characterised by their use mainly in the verbal form, with skills in the literary language generally low or even non-existent. Languages are for the most part learned informally, primarily through communication with parents and/or grandparents. The result is a supersaturated vernacular with a large degree of variation and loans from superstratum languages which falls far from the standard of the language in question.DOI: http://dx.doi.org/10.5128/ERYa6.15

  2. [Mati Erelt. Estonian Language] / Katrin Hiietamm

    Index Scriptorium Estoniae

    Hiietamm, Katrin

    2004-01-01

    Arvustus: Estonian language / [Estonian Academy of Sciences] ; edited by Mati Erelt.Tallinn : Teaduste Akadeemia Kirjastus, 2003. 412, [1] lk. : ill., kaart. (Linguistica Uralica. Supplementary series, 0868-4731 ; vol. 1)

  3. Valued Estonian Music CDs

    Index Scriptorium Estoniae

    2004-01-01

    2002. aastal välja antud eesti muusika heliplaatidest Artur Kapp "Symphonische Werke", Eduard Tubin "Symphonies No.9, No.10 and No.11", "Estonian Preludes", "Eesti heliloojad. Hortus Musicus", "Eesti Muusika Päevad", "Tallinn Saxophone Quartet. Estonian Contemporary Music", "Triskele. Kolga-Jaani vaimulikud rahvalaulud", "Helmekaala. Linnupuu Anne", "Modern Fox mängib Raimond Valgret",

  4. [Marge Rennit. Eesti muuseumid / Estonian museums] / Tapio Mäkeläinen

    Index Scriptorium Estoniae

    Mäkeläinen, Tapio

    2009-01-01

    Tutvustus: Eesti muuseumid = Estonian museums / [Eesti Muuseumiühing ; koostaja Marge Rennit ; tõlkija Tiina Mällo ; toimetaja Ivi Tammaru ; eessõna: Piret Õunapuu ; kujundaja Marek Allvee]. Tallinn : Oomen, 2008

  5. Tallinn - Taani linn / Ago Vallas

    Index Scriptorium Estoniae

    Vallas, Ago

    2002-01-01

    rets. rmt.: Zobel, Rein. Tallinn (Reval) keskajal : linnaehitus 13. - 14. sajandil = Tallinn (Reval) in the middle ages : town building in the 13 th - 14 th centuries. Tallinn : Eesti Entsüklopeediakirjastus, 2001. 213 lk. : ill., kaart

  6. Flux in Tallinn

    Index Scriptorium Estoniae

    2004-01-01

    Rahvusvahelise elektroonilise kunsti sümpoosioni ISEA2004 klubiõhtu "Flux in Tallinn" klubis Bon Bon. Eestit esindasid Ropotator, Ars Intel Inc., Urmas Puhkan, Joel Tammik, Taavi Tulev (pseud. Wochtzchee). Klubiõhtu koordinaator Andres Lõo

  7. Estonian literature / Janika Kronberg

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2003-01-01

    Sisu: Estonian literature - born on the margins of Europe ; Baltic German literature and its impact ; Seeking the contours of a 'truly' Estonian literature ; Literature and an independent Estonia ; Estonian literature in two cultural spheres ; The fifties and sixties ; Literature and congealed time ; A bold new Estonian literature

  8. Tallinn 2011 sai oma margi

    Index Scriptorium Estoniae

    2010-01-01

    Eesti Post andis 9. septembril 2010 välja 2011 aasta Euroopa kultuuripealinnale Tallinnale pühendatud postmargi, mis trükiti SA Tallinn 2011 ja Eesti Posti postmargi kavandamiseks korraldatud konkursi Heino Prunsvelti võidutöö "Lost in Tallinn" järgi

  9. Moda opjat vernulas v Tallinn - Fashion Is Back In Tallinn

    Index Scriptorium Estoniae

    2008-01-01

    Moeüritus Fashion is Back in Tallinn (FIBIT) Viru Keskuses Tallinnas. Gala-etendusel "Sinine, Must ja Valge" esitletud Anu Samarüütel-Longi, Tanel Veenre ja Arne Niidu moekollektsioonidest. Lisaks värvilised fotod

  10. WTC Tallinn kavandab olulist laienemist

    Index Scriptorium Estoniae

    2005-01-01

    World Trade Center Tallinn AS kompleksi arhitektuursest arengust ja laiendamisest. Laienemise ideede saamiseks korraldati 2004. a. arhitektuurikonkurss. Lühidalt OÜ Kavakava, OÜ Alver Trummal Arhitektid ja Arhitektuuribüroo Raivo Puusepp ideedest

  11. MINORITY LANGUAGES IN ESTONIAN SEGREGATIVE LANGUAGE ENVIRONMENTS

    Directory of Open Access Journals (Sweden)

    Elvira Küün

    2011-01-01

    Full Text Available The goal of this project in Estonia was to determine what languages are spoken by students from the 2nd to the 5th year of basic school at their homes in Tallinn, the capital of Estonia. At the same time, this problem was also studied in other segregated regions of Estonia: Kohtla-Järve and Maardu. According to the database of the population census from the year 2000 (Estonian Statistics Executive Office's census 2000, there are representatives of 142 ethnic groups living in Estonia, speaking a total of 109 native languages. At the same time, the database doesn’t state which languages are spoken at homes. The material presented in this article belongs to the research topic “Home Language of Basic School Students in Tallinn” from years 2007–2008, specifically financed and ordered by the Estonian Ministry of Education and Research (grant No. ETF 7065 in the framework of an international study called “Multilingual Project”. It was determined what language is dominating in everyday use, what are the factors for choosing the language for communication, what are the preferred languages and language skills. This study reflects the actual trends of the language situation in these cities.

  12. Overview of the Estonian Biofuels Association activities

    International Nuclear Information System (INIS)

    Hueues, Meelis

    2000-01-01

    Due to global warming and environment pollution because of widespread use of fossil fuels there are already tendencies to stabilize and decrease the consumption of these energy resources and take into use more renewable energy resources. Estonian Biofuels Association (EBA) is a non-profit association, which was founded on 8. of May 1998 in Tallinn. The EBA is an independent and voluntary alliance of its members. Fields of activity of the EBA are by biofuels research, developing and evaluation to engage environmental, biofuels and energy saving. EBA members are: energy consultants, scientists, as well as fuel suppliers, DH-companies, technology suppliers, energy service companies etc. The members of EBA are involved in different projects in Estonia, where biomass are produced and used for heating, where wood, waste, peat, rape oil and biogas resources are examined and put into use, and also projects which deal with energy saving and environment friendly equipment production for using biofuels. During our short experience we have noticed that people in Estonia have become more aware of biomass and their use, so the development of environment friendly and sustainable energetics will continue in Estonia. Available biofuels in Estonia could compete with fossil fuels if burnt rationally with high technology equipment. EBA members are convinced that biomass have perspective and that they could play an important role in improving Estonian economic and environmental situation. Modem biomass combustion devices are taken into use more the faster general wealth increases and EBA can raise people's awareness of bio fuel subject through special, courses and media. We want Estonian energy policy to develop towards widespread use of renewable energy resources, which would save energy and environment improve nation's foreign trade balance and create jobs mainly in rural areas

  13. The Adaptation of the Motivation to Lead Instrument to the Estonian Military Context

    Directory of Open Access Journals (Sweden)

    Antek Kasemaa

    2016-03-01

    Full Text Available Purpose: This study is a contribution to the validation of the Motivation to Lead (MTL scale proposed by Chan and Drasgow (2001 in order to measure three types of motivation to be a leader: affective, social-normative and calculative. This research examines the psychometrical properties of the MTL scale in the Estonian context.Methodology: The sample of 517 military and non-military individuals from the Estonian Defence Forces, Estonian Police and students from Tallinn University participated in the study. The original MTL scale was expanded by with the addition of ideological and patriotic dimensions proposed by Amit and colleagues (2007. The factorial structure of the MTL Scale was analysed by using exploratory and confirmatory factor analysis (respectively N = 170 and N = 347.Findings: The results confirmed that both three- and five- component MTL scales are applicable in the Estonian context; the pool of 35 items was reduced into 25 items with good internal reliability. Moreover, the results showed correlations between leadership self-efficacy and MTL components,and differences between leaders’ and non-leaders’ MTL. The results indicate that the MTL scale can be a reliable and useful instrument to measure leadership motivation in the Estonian military context.Originality: This study is the first to adapt the MTL scale to the Estonian context. In addition, it examines the validity of ideological and patriotic MTL as part of the general MTL construct outside of the Israeli samples.

  14. WTC Tallinn kavandab olulist laienemist

    Index Scriptorium Estoniae

    2005-01-01

    Kunagise Punase RET-i tööstuskvartali arhitektuurseks laiendamiseks korraldas WTC Tallinn AS arhitektuurikonkursi. Ülevaade OÜ Kavakava, arhitektuuribüroo Raivo Puusepp ja OÜ Alver Trummal Arhitektid ideedest ja kavanditest uue kompleksi kasutamisvõimalusteks

  15. Revisiting the Estonian Cyber Attacks: Digital Threats and Multinational Responses

    Directory of Open Access Journals (Sweden)

    Stephen Herzog

    2011-01-01

    Full Text Available In April 2007, the Estonian Government moved a memorial commemorating the Soviet liberation of the country from the Nazis to a less prominent and visible location in Tallinn. This decision triggered rioting among Russian-speaking minorities and cyber terrorism targeting Estonia's critical economic and political infrastructure. Drawing upon the Estonian cyber attacks, this article argues that globalization and the Internet have enabled transnational groups—such as the Russian diaspora—to avenge their grievances by threatening the sovereignty of nation-states in cyberspace. Sophisticated and virtually untraceable political "hacktivists" may now possess the ability to disrupt or destroy government operations, banking transactions, city power grids, and even military weapon systems. Fortunately, western countries banded together to effectively combat the Estonian cyber attacks and minimize their effects. However, this article concludes that in the age of globalization, interdependence, and digital interconnectedness, nation-states must engage in increased cooperative cyber-defense activities to counter and prevent devastating Internet attacks and their implications.

  16. Tallinn toetab tublisid tudengeid stipendiumiga

    Index Scriptorium Estoniae

    2012-01-01

    Tallinna Linnavalitsuse stipendiumist Tallinna Ülikooli ja Estonian Business School'i üliõpilastele. TLÜ: Triin Lauri, Birgy Lorenz, Margit Kaldmäe, Maria Smorževskihh-Smirnova, Karmen Jürisson, Irina Krotova, Külli Külaots, Triinu Maandi ja Rene Mäe; EBS: Laura Roosmaa, Maarja Murumägi ja Kätlin Pulk

  17. Estonian Folklore Archives

    Directory of Open Access Journals (Sweden)

    Risto Järv

    2013-10-01

    Full Text Available This essay presents the history and development of the The Estonian Folklore Archives, the central folklore archives of Estonia established in 1927, and examines its function in relation to the development of online databases. The Archives’ primary purpose has been to make the manuscript materials easily available for researchers working with the collection. The collections consist of manuscripts, sound recordings, photographs, and film and video materials, with an experimental multimedia collection as of 2008. The most extensive project related to the collections was the digitization in 2011-12 of Jakob Hurt’s folklore collection, stored in Kivike, a new file repository and archival infosystem of the Estonian Literary Museum.

  18. Estonian authors / Janika Kronberg, Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2007-01-01

    Tutvustus: Mattheus, Ülo.¡India armastus. Tallinn : Eesti Keele Sihtasutus, 2006 ;¡Teder,¡Tarmo. Onanistid. Tallinn :¡Tuum, 2006 ;¡Hirv, Indrek. Surmapõletaja. Tallinn :¡Tuum, 2006 ;¡Hvostov, Andrei. Henrik : näidend 12-s pildis. Tallinn : Kultuurileht, 2006. (Loomingu Raamatukogu ;¡20) ;¡Traat,¡Mats. Naised ja pojad. Tallinn : Looming, 2006, nr. 2-5 ;¡Traat, Mats. Sarviku armastus. Tallinn :¡Looming, 2006, nr. 11 ;¡Kaardipakk II. Tallinn : Näo Kirik, 2006 ;¡Kivirähk,¡Andrus. Mees, kes teadis ussisõnu. Tallinn :¡Eesti Keele Sihtasutus, 2007 ;¡Õunapuu,¡Ervin. Meie igapäevane jää :¡eesti gootika III. Tallinn : Varrak, 2006 ;¡Ehlvest, Jüri. Palverännak. Tallinn :¡Tuum, 2005

  19. Our Man in Tallinn / Martin Anderson

    Index Scriptorium Estoniae

    Anderson, Martin

    1997-01-01

    Muljeid kontserdist "Warner Classics Gala" "Estonia" kontserdisaalis, kus E. Mägi, E.-S. Tüüri, V. Tormise, U. Sisaski jt. loomingut esitasid "Camerata Tallinn", NYYD Ensemble, RAM, pianist Lauri Väinmaa

  20. Estonian Jumal ‘God’

    OpenAIRE

    Karl Pajusalu; Renate Pajusalu; Ilona Tragel; Ann Veismann

    2016-01-01

    The article focuses on semantics and the use of the word jumal ‘god’ in Estonian. At first, we give an overview of the etymology of jumal ‘god’ and its meanings in Standard Estonian and Estonian dialects. Then we examine jumal ‘god’ as a linguistic unit associated with different constructions and collocational patterns, bleaching of the meaning, and usage in fulfilling different communication goals. In our study we show how jumal ‘god’ can refer literally to the higher power of Christianity o...

  1. Estonian Airi uued soodsad pakkumised

    Index Scriptorium Estoniae

    2010-01-01

    Estonian Airi kodulehelt on võimalik osta lennupileteid koostööpartnerite poolt pakutavatele mandritevahelistele lendudele ning broneerida internetis hotellituba Euroopa suurima hotelli broneerimise teenust pakkuva ettevõtte Booking.com kaudu

  2. Tallinn laseb Ameerika kosilastel linnahalliga vabalt talitada / Tuuli Aug

    Index Scriptorium Estoniae

    Aug, Tuuli

    2010-01-01

    Tallinna linnahalli rendilepingust Ronald S. Lauderi firmaga Tallinn Entertainment LCC. Lepingu kohaselt hakkab linnahalli arendama Tallinna linna ja ameeriklaste loodav ühisettevõte. Et USA osapool annab ettevõttele laenu, loobub Tallinn selle tagasimaksmise aastateks linnahalli tegevuse juhtimisest

  3. Raimo Pullati XX "Vana Tallinn" / Tõnis Liibek

    Index Scriptorium Estoniae

    Liibek, Tõnis

    2009-01-01

    Ilmus professor Raimo Pullati poolt välja antava linnaajalugu ja -kultuuri tutvustava sarja "Vana Tallinn" XX köide. Tšehhi Teaduste Akadeemia raamatukogus toimus Raimo Pullati poolt välja antud entsüklopeedilise suurteose "Johann Christoph Brotze. Estonica" esitlus

  4. Short Outlines of Books by Estonian authors / Rutt Hinrikus

    Index Scriptorium Estoniae

    Hinrikus, Rutt, 1946-

    2007-01-01

    Tutvustus: Miljutina, Tamara. Inimesed minu elus. Tallinn : Varrak, 2007 ; Soosaar, Enn. Isa ja aeg. Tallinn : Kultuurileht, 2007. (Loomingu Raamatukogu ; 5) ; Paju, Imbi. Tõrjutud mälestused. Tallinn : Eesti Entsüklopeediakirjastus, 2007 ; Kaplinski, Jaan. Seesama jõgi. Tallinn : Vagabund, 2007 ; Laanem, Tiina. Väikesed vanamehed. Tallinn : Eesti Päevalehe Kirjastus, 2007 ; Vaarandi, Debora. Aastad ja päevad. Tallinn : Tänapäev, 2006 ; Berg, Maimu. Unustatud inimesed. Tallinn : Tuum, 2007 ; Heinsaar, Mehis. Rändaja õnn. Tallinn : Verb, 2007

  5. Four vision of contemporary Estonian art : the series of exhibitions Estonian art / Heie Treier

    Index Scriptorium Estoniae

    Treier, Heie, 1963-

    2000-01-01

    Neljast eesti kunsti näitusest 2000. a. märtsis-aprillis Tartus ja Tallinnas, nende kuraatoritest: Toomas Vint, Reet Varblane, Peeter Allik, Johannes Saar. Déj́-vu (Tallinn). Naturalism (Tallinn). AIDS kultuuris (Tallinn). Eneseksjäämine (Tartu)

  6. Estonian white paper on energy

    International Nuclear Information System (INIS)

    Hamburg, Arvi

    1997-01-01

    Energy policy, environmental protection and economy form a triangle of tightly linked sectors, and any solution of some energy problem can be solved only in the light of all the above mentioned factors. There are several energy master plans for Estonia, the first of them dating back to the years of the Soviet Union and ending the list with the plan to cover the years up to 2000. By now the basic principles of the Estonian energy policy have been prepared and Estonian Energy Concept is being worked out. The main goal of Estonian energy policy is ensure an effective and environmentally benign energy supply for the country. It means safety in energy supply, effective production and supply together with sufficient environmental protection. Energy Council in the role of an advisory voluntary organization for inspection of the energy system and finding measures to improve its efficiency is established with parliament members included. The Estonian Energy Research Institute and the Oil--Shale Research Institute serve as a scientific advisory board for the government in energy policy. It's important to emphasise that privatisation is no panacea, solving all the problems, and therefore we are facing hard to move in the right direction, satisfying all the consumers of energy

  7. Russification of non-Estonian pupils in Tallinn / Mart Rannut, Ülle Rannut

    Index Scriptorium Estoniae

    Rannut, Mart, 1959-

    2010-01-01

    Analüüsitakse õpilaste kodukeeli, nende kvaliteeti ja kasutust. Võrreldakse kahte suurt rühma (ukraina ja valgevene) ja kahte islami taustaga rühma (aserbaidžaani ja tatari), et analüüsida keele säilimise ja kao faktoreid

  8. ESADSE, Option Design: Workshop Tallinn For All

    Index Scriptorium Estoniae

    2011-01-01

    Rahvusvahelisest koostööprojektist "Cities For All - Tallinn For All". Projekti viis läbi Eesti Disainerite Liit. Ilona Gurjanova organiseeritud töötubades osalesid ajavahemikus september 2010 - september 2011 järgmised kõrgkoolid: Eesti Kunstiakadeemia, Mainori Kõrgkool, Tartu Ülikooli Pärnu Kolledž, Aalto Ülikool (Soome), Saint-Étienne Higher School of Art and Design (Prantsusmaa)

  9. Sustainable development and Estonian energetics

    International Nuclear Information System (INIS)

    Lausmaa, T.

    1997-01-01

    This conference was held 14 Nov 1997 in Tallinn, Estonia. The conference stressed the importance of the diminishing the negative impact of energy production on the environment. The Government and the Parliament should ensure the composing of short and long term master plans with the public participation for all sectors of the economy, based on the principles of sustainable development, the involved international treaties and the Sustainable Development Framework Act

  10. Short outlines of books by Estonian authors / Janika Kronberg, Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2006-01-01

    Arvustus: Krull, Hasso. Loomise mõnu ja kiri. Tallinn : Kultuurileht, 2006 ; Aleksejev, Tiit. Valge kuningriik. Tallinn : Varrak, 2006 ; Baturin, Nikolai. Sõnajalg kivis. Tallinn : Eesti Raamat, 2006 ; Nõu, Helga. Ood lastud rebasele. Tallinn : Eesti Keele Sihtasutus, 2006 ; Kaus, Jan. Tema. Tallinn : Tuum, 2006 ; Krull, Hasso. Talv. Tallinn : Tuum, 2006 ; Soomets, Triin. Väljas. Tallinn : Tuum, 2006 ; Kareva, Doris. Aja kuju. Tallinn : Verb, 2006 ; Vint, Toomas. Topeltvalguses. Tallinn : Eesti Keele Sihtasutus, 2005 ; Bristol, Piret. Sõud. Tallinn : Eesti Keele Sihtasutus, 2005 ; Valton, Arvo. Taltsutatud lammas : jutud. Tallinn : Kirjastuskeskus, 2005 ; Kivastik, Mart. Külmetava kunstniku portreed. Viinistu triloogia. Tartu : Väike Öömuusika, 2006

  11. Tallinn rekonstrueerib : juhtumid kahe objektiga / Triin Ojari

    Index Scriptorium Estoniae

    Ojari, Triin, 1974-

    1998-01-01

    1960. a. ehitatud hotell Tallinna (arhitektid Peeter Tarvas, Toivo Kallas) ümberehitus hotelliks Grand Mercure Tallinn. Ümberehituse autor - Kalle Rõõmuse arhitektuuribüroo (I. Vainu, A. Kukke, K. Smirnov, T. Pakri). 1939. a. valminud Pirita restorani (Regati baar) ja autobusside lõppjaama hoone (arhitekt Arthur Jürvetson) - Tallinnas ainus säilinud funktsionalistliku rannaarhitektuuri näide - ümberehitus Regati Maja Ärikeskuseks. Ümberehitusprojekt valmis Laansoo Grupis, arhitektid Anne Kose, Andrus Sisask. Kommentaarid Kalle Rõõmus, Andrus Sisask.

  12. Cultural Value Differences, Value Stereotypes, and Diverging Identities in Intergroup Conflicts: The Estonian Example

    Directory of Open Access Journals (Sweden)

    Henrik Dobewall

    2011-07-01

    Full Text Available

    An examination of the relationship between cultural values, value stereotypes and social identities in Estonia, where intergroup conflicts triggered riots in the capital
    Tallinn in April 2007, using data from the European Social Survey on cultural differences and value trends as the background to a survey exploring perceived
    group values and assessed social identities among ethnic Estonians and members of the Russian-speaking minority. The study, conducted in summer 2008, found
    agreement across both ethnic groups about the values of a typical group member, but no accuracy in their attribution. The Estonian students (n = 152 avoided
    Eastern-European identification, while the Russian-speaking students (n = 54 did not want to give up Estonia’s Soviet past. We found that attributed rather than
    self-rated value differences between groups caused the conflicts, whilst diverging identities were found to make value stereotypes more extreme.

  13. Short outlines of books by Estonian authors / Brita Melts, Rutt Hinrikus and Janika Kronberg

    Index Scriptorium Estoniae

    Melts, Brita

    2013-01-01

    Arvustus: Baturin, Nikolai. Lendav hollandlanna. Tallinn : Eesti Raamat, 2012 ; Friedenthal, Meelis. Mesilased. Tallinn : Varrak, 2012 ; Kaus, Jan. Koju. Tallinn : Tuum, 2012 ; Kaldmaa, Kätlin. Armastuse tähestik. Tallinn : NyNorden, 2012 ; Kivastik, Mart. Vietnami retsept. Tartu : Väike Öömuusika, 2012 ; Õnnepalu, Tõnu. Kuidas on elada. Tallinn : Varrak, 2012 ; Õnnepalu, Tõnu. Mandala. Tallinn : Varrak, 2012 ; Riismaa, Kaur. Rebase matmine. Tallinn : Näo Kirik, 2012 ; Vadi, Urmas. Tagasi Eestisse. Saarde-Pärnu : Jumalikud Ilmutused, 2012 ; Vint, Toomas. Avalikult abielust. Tallinn : Tulikiri, 2012 ; Kangro, Maarja. Dantelik auk. Tallinn : Eesti Keele Sihtasutus, 2012 ; Kaevats, Mihkel. Ungari kirsid & teisi luuletusi. Tallinn : Pegasus, 2012.

  14. Estonian Air to overhaul strategy / Matt Withers

    Index Scriptorium Estoniae

    Withers, Matt

    2008-01-01

    Estonian Air on majanduslikes raskustes, mida aitaks leevendada riigipoolne toetus. Majandus- ja kommunikatsiooniminister Juhan Parts leiab, et riik peaks omama lennufirma juhatuses esimehe kohta, et mõjutada rohkem vastuvõetavaid otsuseid ja investeeringuid

  15. Estonian Golf & Country Club / Urmas Oja

    Index Scriptorium Estoniae

    Oja, Urmas, 1981-2012

    2005-01-01

    Konkursil "Eesti parim puitehitis 2005" pälvis voodrilaua eripreemia Jõelähtme Estonian Golf & Country Club'i katus. Arhitekt Andres Siim. Sisearhitekt Juta Lember. Konstruktor: AS Resand. 11 värv. ill

  16. On the System of Person-Denoting Signs in Estonian Sign Language: Estonian Name Signs

    Science.gov (United States)

    Paales, Liina

    2010-01-01

    This article discusses Estonian personal name signs. According to study there are four personal name sign categories in Estonian Sign Language: (1) arbitrary name signs; (2) descriptive name signs; (3) initialized-descriptive name signs; (4) loan/borrowed name signs. Mostly there are represented descriptive and borrowed personal name signs among…

  17. Tallinn Fashion Week algas moeillustraatorite näitusega

    Index Scriptorium Estoniae

    2011-01-01

    Näitus "Uus Põhjamaade moeillustratsioon" Rotermanni kvartalis 18. sept.-ni. Osalejad Eestist, Soomest ja Rootsist loetletud. Näituse kujundaja Tuuli Aule. Väljapaneku loovjuhi Toomas Volkmanni selgitus. Tallinn Fashion Week'i põhiprogrammist

  18. Air Baltic: Estonian Air on nurka surutud / Teele Tammeorg

    Index Scriptorium Estoniae

    Tammeorg, Teele

    2010-01-01

    Air Balticu asepresidendi Janis Vanagsi hinnangul on Estonian Air aastaid jätnud tähelepanuta oma peamised turismiturud ning on praegu halvas seisus. Air Baltic on endiselt huvitatud Estonian Airi ostust. Majandus- ja kommunikatsiooniminister Juhan Partsi seisukoht

  19. Tallinn andis õpilastele preemia alkovaba noortejoogi leiutamise eest / Kai Maran

    Index Scriptorium Estoniae

    Maran, Kai

    2014-01-01

    Ettevõtluskonkursi ENTRUM raames sai Tallinna linna eripreemia "Parim Tallinna Start-up 2014" Tallinna Vanalinna Hariduskolleegiumi ja Estonian Business School'i koolinoorte start-up Passion Group idee eest toota tervisliku ja alkoholivaba mahlajookide seeria

  20. Short outlines of books by Estonian authors / Janika Kronberg, Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2004-01-01

    Tutvustus: Talvik, Heiti. Legendaarne. Tartu : Ilmamaa, 2004 ; Pilter, Lauri. Lohejas pilv : romaan lühijuttudes. Tallinn : Tuum, 2004 ; Raud, Rein. Hector ja Bernard. Tallinn : Tuum, 2004 ; Ruben, Aarne. Elajas trepi eelastmel. Tallinn : Tuum, 2004 ; fs. 2004. Tallinn : Tuum, 2004 ; Normet, Dagmar. ...ainult võti taskus. Tallinn : Varrak, 2004 ; Künnap, Asko. Ja sisalikud vastasid (kolmes kirjas). Tallinn : Näo Kirik, 2003 ; Kall, Toomas. Armas aeg. Tallinn : Tänapäev, 2004 ; Õunapuu, Ervin. Eesti gootika II. Tallinn : Umara, 2004 ; Soomets, Triin. Leping number 2 : luuletusi 2000-2003. Tallinn : Tuum, 2004 ; Tuulik, Jüri. Linnusita : [jutustused, lühijutud, näidendid ja vested]. Tallinn : Maalehe Raamat, 2004 ; Raudam, Toomas. Teie. Tallinn : Eesti Keele Sihtasutus, 2003 ; Härm, Viiu. Keegi teeline : luuletused. Tallinn : Varrak, 2004 ; Härm, Viiu. Õhuaken : romaan. Tallinn : Varrak, 2004

  1. Short Outlines of Books by Estonian Authors / Janika Kronberg, Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2009-01-01

    Arvustus: Karusoo, Merle. Kui ruumid on täis : Eesti rahva elulood teatritekstides 1982-2005. Tallinn : Varrak, 2008 ; Aleksejev, Tiit. Palveränd. Tallinn : Varrak, 2008 ; Saat, Mari. Lasnamäe lunastaja. Tallinn : Tuum, 2008 ; Tarand, Mari. Ajapildi sees. Tartu : Ilmamaa, 2008 ; Ojamaa, Liisi. Jõgi asfaldi all. Tallinn : Varrak, 2008 ; Kaalep, Ain. Muusad ja maastikud. Luuletused aastaist 1945-2008. Tallinn : Tänapäev, 2008 ; Toona, Elin. Ella. Tallinn : Kultuurileht, 2008 ; Hellerma, Kärt. Sinine missa. Valik reisikirju. Tallinn : Tuum, 2008 ; Raud, Rein. Vend. Tallinn : Tuum, 2008 ; Kruusa, Kalju. Pilvedgi mindgi liigutavadgi. Tallinn : Koma, 2008

  2. Short outlines of books by Estonian authors / Brita Melts, Rutt Hinrikus, Maret Vaher and Arno Oja

    Index Scriptorium Estoniae

    Melts, Brita

    2014-01-01

    Arvustus: Kronberg, Janika. Rännud kuue teejuhiga. Tallinn : Go Group, 2013 ; Kallaste, Helen. Kogutud hetked. Tallinn : Verb, 2012 ; Ahi, Eda. Gravitatsioon. Tallinn : Verb, 2013 ; Heinsaar, Mehis. Ülikond. Tallinn : Menu Kirjastus, 2013 ; Kann, Kaja. Eratee. Tallinn : Hea Lugu, 2013 ; Kivirähk, Andrus. Maailma otsas. Tallinn : Eesti Keele Sihtasutus, 2013 ; Kurvitz, Robert. Püha ja õudne lõhn. Tallinn : ZA/UM, 2013 ; Mattheus, Ülo. Tema salajane palve. Tallinn : Tuum, 2013 ; Kangro, Maarja. Dantelik auk. Tallinn : Eesti Keele Sihtasutus, 2012

  3. Short outlines of books by Estonian authors / Janika Kronberg, Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2008-01-01

    Arvustus: Kareva, Doris. Lõige. Tallinn : Verb, 2007 ; Õnnepalu, Tõnu. Flandria päevik. Tallinn : Varrak, 2007 ; Mihkelson, Ene. Katkuhaud. Tallinn : Varrak, 2007 ; Vint, Toomas. Mäluauguga naine. Tallinn : Eesti Keele Sihtasutus, 2007 ; Pervik, Aino. Suleline, Puhuja ja must munk. Tallinn : Tänapäev, 2007 ; Mutt, Mihkel. Siseemigrant. Novellid Rui taevalike kommentaaridega. Tallinn : Fabian, 2007 ; Nõlvak, Valentine. Ellujääja mälestused. Tallinn : Varrak, 2007 ; Tungal, Leelo. Täisminevik. Tallinn : Tänapäev, 2007 ; Kangro, Maarja. Tule mu koopasse, mateeria. Tallinn : Eesti Keele Sihtasutus, 2007

  4. Short outlines of books by Estonian authors / Janika Kronberg and Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2010-01-01

    Arvustus: Käsper, Kalle. Buridanid. I-V. Tallinn : Eesti Keele Sihtasutus, 2005-2010 ; Kivastik, Mart. Eesti asjad. Tartu : Kaks Musketäri, 2010 ; Kangro, Maarja. Ahvid ja solidaarsus. Tallinn : Eesti keele Sihtasutus, 2010 ; Põder, Rein. Unustatud. Tallinn : Eesti Raamat, 2010 ; Kesküla, Kalev. Elu sumedusest. Tallinn : Tuum, 2009 ; Pilv, Aare. Ramadaan. Tallinn : Tuum, 2010

  5. Stocks of organic carbon in Estonian soils

    Directory of Open Access Journals (Sweden)

    Kõlli, Raimo

    2009-06-01

    Full Text Available The soil organic carbon (SOC stocks (Mg ha–1 ofautomorphic mineral (9 soil groups, hydromorphic mineral (7, and lowland organic soils (4 are given for the soil cover or solum layer as a whole and also for its epipedon (topsoil layer. The SOC stocks for forest, arable lands, and grasslands and for the entire Estonian soil cover were calculated on the basis of the mean SOC stock and distribution area of the respective soil type. In the Estonian soil cover (42 400 km2, a total of 593.8 ± 36.9 Tg of SOC is retained, with 64.9% (385.3 ± 27.5 Tg in the epipedon layer (O, H, and A horizons and 35.1% in the subsoil (B and E horizons. The pedo-ecological regularities of SOC retention in soils are analysed against the background of the Estonian soil ordination net.

  6. Digimodernistlik eesti kirjanik / The Digimodernist Estonian Writer

    Directory of Open Access Journals (Sweden)

    Piret Viires

    2013-06-01

    Full Text Available The article examines the cultural situation following postmodernism in the first decade of the 21st century. To characterise this situation, the umbrella term “post-postmodernism” is used, as well as “neomodernism”, “altermodern”, “metamodernism”, “hypermodernity”, “performatism”, “critical realism” etc. All these approaches are, in a wider sense, united by their aim of opposing postmodernist cynicism and irony, and bringing back truth, simplicity and clarity. It has also been found that literature has returned or is returning to realism, and various cultural phenomena are emerging, which have been designated by the concept “new sincerity”.In descriptions of the current cultural situation, this trend seeking truth and simplicity is supported by approaches which emphasise the significance of technological developments during the last decade. A prominent figure here is Alan Kirby, who launched the term “digimodernism”, mainly linked with the adaptation and spread of Web 2.0 at the beginning of the 21st century: the blogosphere, Wikipedia, Twitter and Facebook.The article seeks answers to the question of whether we can talk about digimodernism in Estonian literature in the 2000s. In the 1990s Estonian writers were quite reluctant to undertake computer-technological experiments, and there are only a few examples of Estonian digital literature, whereas a change occurred in the 2000s. Many Estonian writers have had and still have their own blogs and surprisingly many have joined Facebook. The term “twitterature” is also familiar to Estonian writers. The article tackles the dominant topics in the blogs of Estonian writers and analyses their possible collective creative work on Facebook. A question is raised as to whether it is possible that the fragmentary narrative structure of blogs and Facebook has influenced mainstream literature.The article concludes that one essential change in Estonian literature in the

  7. DNA Fingerprinting Eastern Redbud Cultivars (Cercis canadensis) Using SSR Markers

    Science.gov (United States)

    In this study we present data for a subset of SSR loci, 76 out of the 130 high-quality loci, which were selected out of hundreds of SSR loci identified from a SSR-enriched library. SSR markers are abundant in eukaryotic genomes and are highly reproducible. Previously, we have used SSR markers to e...

  8. Short outlines of books by Estonian authors / Brita Melts, Rutt Hinrikus and Janika Kronberg

    Index Scriptorium Estoniae

    Melts, Brita

    2012-01-01

    Arvustus: Helme, Peeter. Varastatud aja lõpus. Tallinn : Tuum, 2011 ; Jõerüüt, Jaak. Kõik luuletused. Tallinn : Eesti Keele Sihtasutus, 2011 ; Kareva, Doris. Sa pole üksi. Tallinn : Verb, 2011 ; Paju, Imbi. Soome lahe õed : vaadates teiste valu. Tallinn : Hea lugu, 2011 ; Pervik, Aino. Matlena teekond. Tallinn : Mustvalge Kirjastus, 2010 ; Raud, Rein. Hotell Amalfi. Tallinn : Tuum, 2011 ; Sommer, Lauri. Räestu raamat. Tallinn : Menu Kirjastus, 2012 ; Kasemaa. Andrus. Leskede kadunud maailm. Tallinn : Varrak, 2012 ; Teede, Andra. Käigud. Pärnu : Jumalikud Ilmutused, 2011

  9. Valued Estonian Music CDs / Igor Garshnek

    Index Scriptorium Estoniae

    Garšnek, Igor, 1958-

    2003-01-01

    2002. aastal välja antud eesti muusika heliplaatidest Arvo Pärt "Summa", Lepo Sumera "Chamber Music", "Baltic Voices 1.", "Sequenzen - Europäische Orgelmusik des 20. Jahrhundrets mit...", "El silenco ئ Silence. Kuldar and Marje Sink. Songs of Mother and Son", "Riho Sibul. Estonian Dream Big Band", "Rull's Royce ئ Rull's Choice"

  10. English in the Estonian Multicultural Society.

    Science.gov (United States)

    Fonzari, Lorena

    1999-01-01

    Argues that the spread of English is not closely associated to a discourse of linguistic imperialism, basing the thesis on a case study in Estonia. Surveys of three generations of Estonians indicated that all welcomed English as a means of communications and technology in the modern world, reacting against the imposition of Russian language and…

  11. Resource and utilization of Estonian hydropower

    International Nuclear Information System (INIS)

    Raesaar, P.

    2005-01-01

    An overview of the Estonian hydropower resources and their utilization at present as well as prospective for the future are presented in this paper. A short overview of advantages of small hydropower stations and related issues is given. Some technological aspects are treated briefly. (authors)

  12. Transformational Leadership in the Estonian Defence Forces

    Directory of Open Access Journals (Sweden)

    Antek Kasemaa

    2015-06-01

    Full Text Available Purpose – The study is a contribution to the validation of the 15 items and 5 subscales Transformational Leadership Scale (TLS proposed by Rafferty and Griffin (2004. Design/methodology/approach – The sample includes participants from different levels of the Estonian Defence Forces (EDF military hierarchy (N=2570. The structure of the TLS was examined by using exploratory and confirmatory factor analyses. Additionally ANOVA was used to compare the results between different subsamples. Findings – TLS showed satisfactory reliability. Confirmatory factor analyses found TLS as valid five dimensions instrument to measure transformational leadership in the Estonian military context. Different management levels showed different emphases among the dimensions of transformational leadership. Research and practical limitations/implications – TLS will be an important tool to use in transformational leadership research in the Estonian military context and beyond. Additionally, the current research contributes to the development of alternative measurement tools besides the most commonly used MLQ. The limitation of the work will be the rather homogenous sample from the Estonian military, however it will open the door for the subsequent research using different samplings. Originality/value – The current research found TLS to be a reliable and valid instrument, very short and therefore easy to administrate, having the possibility to use it with five dimensional and as one general transformational instrument as well.

  13. Swedish-Estonian energy forest research cooperation

    International Nuclear Information System (INIS)

    Ross, J.; Kirt, E.; Koppel, A.; Kull, K.; Noormets, A.; Roostalu, H.; Ross, V.; Ross, M.

    1996-01-01

    The Organization of Estonian energetic economy is aimed at cutting the usage of oil, gas and coal and increasing the local resources firewood, oil-shale and peat for fuel. The resources of low-valued firewood-brushwood, fallen deadwood etc. are available during the following 10-15 years, but in the future the cultivation of energy forest (willow) plantations will be actual. During the last 20 years the Swedish scientists have been extensively studying the willow forest selection, cultivation and use in energetics and waste water purification systems. A Swedish-Estonian energy forest research project was started in 1993 between the Swedish Agricultural University on one hand and Toravere Observatory, Institute of Zoology and Botany, Estonian Academy of Sciences and Estonian Potato Processing Association on the other hand. In spring 5 willow plantations were established with the help of Swedish colleagues and obtained from Sweden 36000 willow cuttings. The aim of the project: a) To study experimentally and by means of mathematical modelling the biogeophysical aspects of growth and productivity of willow plantations in Sweden and Estonian climatological conditions. b) To study the possibility of using the willow plantations in waste waters purification. c) To study the economical efficiency of energy forest as an energy resource under the economic and environmental conditions of Estonia. d) To study the economic efficiency of willow plantations as a raw material for the basket industry in Estonia. e) To select the most productive and least vulnerable willow clones for practical application in energy plantations. During 1993 in all five plantations detailed analysis of soil properties has been carried out. In the plantation at Toravere Observatory phytometrical measurements were carried out - the growth of plant biomass leaf and stem area, vertical distribution of dry matter content, biomass and phyto area separately for leaves and stems has been performed. Some

  14. Tallinn makes most intellegent city list / Ella Karapetyan

    Index Scriptorium Estoniae

    Karapetyan, Ella

    2010-01-01

    Intelligent Community Forum (ICF) kuulutas välja 2010. aastal Smart21 nimekirja pääsenud kogukonnad. Tallinn asub nimekirjas 7. kohal. Nimekirja jõuavad suure majandusliku ja sotsiaalse arengu läbi teinud kogukonnad. 21. mail kuulutatakse välja võitnud kogukond

  15. Tallinn soovib linnahalli Ameerika investoritega ikka edasi minna / Raimo Poom

    Index Scriptorium Estoniae

    Poom, Raimo

    2010-01-01

    Tallinna linnavalitsus ootab enne otsustamist, kas pikendada Tallinna linnahalli arendamiseks sõlmitud eellepingut Ameerika ettevõttega Tallinn Entertainment LLC, Ameerika investorite delegatsiooni visiiti Tallinna. Rahandusminister Jürgen Ligi vastusest Tallinna abilinnapea Taavi Aasa kirjale, milles oli ettepanek kaaluda võimalust anda riigi garantii linnahalli arendamise projektile

  16. Kas Tallinn vajab uut brändi? / Andreas Sepp

    Index Scriptorium Estoniae

    Sepp, Andreas

    2010-01-01

    Tallinnas viibinud saksa loomemajanduse õppejõudude Bernd Hartmanni ja Jan Runge sõnul on kultuuripealinna aasta hea võimalus linnakuvandi parandamiseks. Nende arvates võiks Tallinn kultuuripealinna raames anda noortele disaineritele ja filmitegijatele loomingu eksponeerimiseks piisavalt võimalusi ning keskenduda kohaliku kultuurielu parandamisele

  17. Hoogsalt moodne Tallinn Fashion Week / Jaanika Terasmaa : intervjueerinud Annely Martin

    Index Scriptorium Estoniae

    Terasmaa, Jaanika

    2010-01-01

    Tallinn Fashion Week kestab 7. novembrini. Üks moenädala korraldajaid Jaanika Terasmaa, sellest, mida moenädal pakub tavainimesele ja kui oluline on selline ettevõtmine moeloojatele, moenädala eesmärgist

  18. Prevalence of cardiovascular disease risk factors in Tallinn, Estonia

    Directory of Open Access Journals (Sweden)

    Margit Kaldmäe

    2017-01-01

    Conclusions: The study established a high prevalence of cardiovascular disease risk factors in Estonian adults (20–65 years of age. Younger portion of the population and some extent ethnic considerations should be taken into account when designing future studies, health prevention activities and interventions.

  19. Eesti Metodisti Kirik : Balti Misjoni Keskus, Narva mnt. 51, Tallinn = Estonian Methodist Church / Vilen Künnapu

    Index Scriptorium Estoniae

    Künnapu, Vilen, 1948-

    2000-01-01

    Projekteerija Arhitektuuribüroo Künnapu & Padrik. Arhitektid Vilen Künnapu, Ain Padrik. Konstruktsioonide autor Andres Laur. Sisekujundajad Argo ja Katrin Vaikla. Projekt 1994, valmis 2000. Kirik on klaasja pikliku koolitiivaga (teoloogiline seminar). 7 ill.: I korruse plaan, lõige, välis- ja sisevaated

  20. Medieval socialist realism : Representations of Tallinn Old Town in Soviet Estonian Feature Films, 1969-1972 / Eva Näripea

    Index Scriptorium Estoniae

    Näripea, Eva

    2004-01-01

    Tallinna keskaegse vanalinna kujutamine Nõukogude Eesti mängufilmides : "Viimne reliikvia" (režii Grigori Kromanov, Tallinnfilm 1969), "Varastati Vana Toomas" (režii Semjon Shkolnikov, Tallinnfilm 1970), "Kolme katku vahel" (režii Virve Aruoja, Eesti Telefilm 1970), "Don Juan Tallinnas" (režii Arvo Kruusement, Tallinnfilm 1971), "Verekivi" (režii Madis Ojamaa, Tallinnfilm 1972)

  1. Short outlines of books by Estonian authors / Brita Melts and Rutt Hinrikus

    Index Scriptorium Estoniae

    Melts, Brita

    2011-01-01

    Arvustus: Vadi, Urmas. Kirjad tädi Annele. Pärnu : Jumalikud Ilmutused, 2010 ; Sommer, Lauri. Kolm yksiklast. Tallinn : Menu Kirjastus, 2010 ; Hargla, Indrek. Apteeker Melchior ja Oleviste mõistatus. Tallinn : Varrak, 2010 ; Hargla, Indrek. Apteeker Melchior ja Rataskaevu viirastus. Tallinn : Varrak, 2010 ; Pihelgas, Carolina. Metsas algavad hääled. Tallinn : Eesti Keele Sihtasutus, 2010

  2. SSR

    African Journals Online (AJOL)

    user

    MapDraw: a Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Hereditas. (Beijing). 25: 317–321. Liu SB, Zhou RH, Dong YC, Li P, Jia JZ (2006). Development utilization of introgression lines using a synthietic wheat as donor. Theor. Appl. Genet. 112: 1360-1373. Lu CM, Yang WY ...

  3. Vitality of the Estonian forests (results of the inventory and research)

    International Nuclear Information System (INIS)

    Karoles, K.

    1991-01-01

    Factors affecting Estonian forests are: The environmental, specially atmospheric pollution, - the foundation of new forests as monoculture on unsuitable locations, - mechanical damages by unsuitable forest machinery, - unfavourable water conditions, - Heterobasidion or Armillaria rot roots. Local damages in consequence of air pollutants are distributed in environments of Tallinn, Kivioli, Kohtla-Jaerve and the thermal power stations (Narva), where the SO 2 -content in the air is on the average higher than 50 (80) μg/m 3 . Pine forests on dry sand soils (600 ha damaged in 1989) and the older spruce forests show the new type of forest decline. High Al-ion concentration, disturbances of the Ca-Mg-metabolism, an extreme nutrient deficit, (specially N-deficit) and periodical water deficit as well as pathogenic fungi are damaging the trees. Spruces show nonspecific defoliation, needle necrosis, needlefall, occurence of fungal diseases. More damaged are the spruce forests in regions with basic precipitations and high sulphur-deposition. (orig./UWA) [de

  4. Short outlines of books by Estonian authors / Janika Kronberg, Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2008-01-01

    Arvustus: Afanasjev, Vahur. Kosmos. Tallinn : Jutulind, 2008 ; Hvostov, Andrei. Võõrad lood. Tallinn : Tänapäev, 2008 ; Lauli, Olle. Niguliste õpilased. Tallinn : Verb, 2007 ; Rooste, Jürgen. Tavaline eesti idioot. Pärnu : Ji, 2008 ; Talvet, Jüri. Silmad peksavad une seinu. Tartu : Ilmamaa, 2008 ; Beekman, Aimée. Proovielu. Tallinn : Varrak, 2008 ; Kivi, Aita. Lähedal. Tallinn : Ajakirjade Kirjastus, 2008 ; Beekman, Vladimir. Alles see oli. Tallinn : Tänapäev, 2008 ; Viiding, Elo. Püha Maama. Tallinn : Tuum, 2008 ; Nõu, Helga. Peaaegu geenius ehk Schrödingeri kassi otsimas. Tallinn : Atlex, 2008 ; Unt, Lii. Parim näitleja linnas. Varanasi päevaraamat. Tallinn : Eesti Ekspressi Kirjastus, 2007 ; Sild, Ivar. Tantsiv linn. Tallinn : Tuum, 2007

  5. Short outlines of books by Estonian authors / Janika Kronberg, Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2004-01-01

    Tutvustus: Baturin, Nikolai. Kentaur. Tallinn : Eesti Raamat, 2003 ; Kaplinski, Jaan. Isale. Tallinn : Varrak, 2003 ; Jaks, Ilmar. Pimedus. Tartu : Ilmamaa, 2003 ; Ivask, Ivar. Tähtede tähendust tunda. Tartu : Ilmamaa, 2003 ; Vint, Toomas. Minu abielu prostituudiga. Tallinn : Eesti Keele Sihtasutus, 2003 ; Ehin, Kristiina. Luigeluulinn. Tallinn : Huma, 2004 ; Kivisildnik. Päike, mida sa õhtul teed? Tallinn : Perioodika, 2003 ; Kivisildnik. Rahvuseepos Kalevipoeg ehk armastus. Tallinn : Tuum, 2003 ; Veidemann, Rein. Lastekodu. Tallinn : Eesti Keele Sihtasutus, 2003 ; Park, Eeva. Lõks lõpmatuses. Tallinn : Tänapäev, 2003 ; Traat, Mats. Islandi suvi. Tartu : Ilmamaa, 2003 ; Kauksi Ülle. Uibu. Tartu : Kauksi Ülle Mänedsment, 2003 ; Kross, Jaan. Kallid kaasteelised. Tallinn : Eesti Keele Sihtasutus, 2003 ; Undusk, Jaan. Quevedo. Tallinn : Perioodika, 2003

  6. Human Capital Reporting and its Linkage with Key Performance Indicators of Companies: Evidence from Estonian Companies Listed on Nasdax OMX Baltic

    Directory of Open Access Journals (Sweden)

    Emilia Startseva

    2015-12-01

    Full Text Available Today the subject of non-financial accounting and reporting has become very popular. Human Capital Reporting (HCR has become a vital part of everyday business activities of companies across the world. Most companies declare that human capital is the most important factor of their competiveness, which is also reflected in their CSR reports by incorporated social disclosures. The subject of the present research is human capital reporting and its linkage with the performance indicators of companies. The aim of this research is to establish whether there is a linkage between HCR and financial performance indicators of Estonian companies listed on the Nasdaq OMX Baltic (Tallinn market. The authors calculated various ratios and carried out HCR scoring to examine the presence of linkage between HCR reporting and companies’ financial performance. The results demonstrate that there is no direct relationship between the level of HCR and the financial performance of selected companies.

  7. Tallinna sünagoog = The Tallinn Synagogue

    Index Scriptorium Estoniae

    2008-01-01

    Tallinna sünagoogi (Karu 16, Tallinn) sisekujundusest. Arhitektid: Lembit-Kaur Stöör ja Tõnis Kimmel, sisearhitektid: Liis Lindvere ja Raili Paling, ornamentika töötas välja Martin Lazarev (KOKO Arhitektid). Akustik: Linda Madalik. Sisearhitektidest, nende tähtsamad tööd. I-IV korruse plaan, 3 värv. välis- ja 8 sisevaadet, fotod sisearhitektidest

  8. Tallinna Inglise kolledzhi spordihoone = The Tallinn English College's sports building

    Index Scriptorium Estoniae

    2008-01-01

    Tallinna Inglise kolledzhi spordihoone (Estonia pst. 10, Tallinn) sisekujundusest. Arhitektid: Lembit-Kaur Stöör ja Margus Maiste (KOKO Arhitektid), sisearhitektid: Liisi Murula (Mari OÜ), Liis Lindvere ja Raili Paling, ornamentika: Martin Lazarev (KOKO Arhitektid). Sisearhitektidest, nende tähtsamad tööd. 5 korruse plaani, 2 värv. välis- ja 11 sisevaadet, fotod sisearhitektidest

  9. Mart Remmeli "geograafilised figuurid" / Arvo Krikmann

    Index Scriptorium Estoniae

    Krikmann, Arvo, 1939-2017

    2005-01-01

    Refereeritakse broshüüri Remmel, Mart. Large data bases in quantitative history : some handling techniques. Tallinn : Academy of Sciences of the Estonian S.S.R., 1979. (Preprint ; KKI-12) inimgeograafia teooria vaatevinklist ning kommenteeritakse seda Eesti folkloorinähtuste geograafilist levikut puutuva materjaliga

  10. Short outlines of books by Estonian authors / Brita Melts, Rutt Hinrikus, Maire Iro and Maret Vaher

    Index Scriptorium Estoniae

    2013-01-01

    Arvustus: Pärtna, Maarja. Läved ja tüved. Tallinn : Eesti Keele Sihtasutus, 2013 ; Sauter, Peeter. Ära jäta mind rahule. Tallinn : P. Sauter, 2012 ; Viiding, Elo. Teised. Tallinn : Tuum, 2012 ; Mathura. Kumalasepäev. Lelle : Allikaäärne, 2012 ; Kadastik, Mart. Kevad saabub sügisel. Tallinn : Varrak, 2013 ; Mutt, Mihkel. Kooparahvas läheb ajalukku. Tallinn : Fabian, 2012 ; Hargla, Indrek. Apteeker Melchior ja Pirita kägistaja. Tallinn : Varrak, 2013 ; Raud, Rein. Rekonstruktsioon. Tallinn : Mustvalge Kirjastus, 2012 ; Toona, Elin. Into exile : a life story of war and peace. Woodsville (N.H.) : Lakeshore Press, 2013 ; Berg, Maimu. Moemaja. Tartu : Petrone Print, 2012 ; Kareva, Doris, Rooste, Jürgen. Elutants. Tallinn : Verb, 2013 ; Ehin, Kristiina. Paleontoloogi päevaraamat. Tartu : Petrone Print, 2013 ; Maria Lee. Stereomeetria. Tallinn : Verb, 2012

  11. Seltsi muuseumist riigi keskmuuseumiks: ikka ajutiste lahendustega / Changes in the Estonian National Museum from 1909 to the present.

    Directory of Open Access Journals (Sweden)

    Krista Aru

    2011-01-01

    Full Text Available Changes in the Estonian National Museum from 1909 to the presentThe Estonian National Museum was founded in Tartu in 1909 as part of the national movement. With its activities and connections in society, the ENM helped create Estonian society, the nation’s collective memory and identity.The ENM has always been – despite the changing locations, names, and content – one of the symbols of national identity. But at the same time, ENM has never had its own building designed specially for the museum’s purposes.Since 1909 there have been several attempts to establish a home for the ENM. At first (1909–1923, Estonian society wanted to establish the museum in the center of Tartu. The museum was intended to become a key institution of the growing nation and establishing the nation’s identity.At last in 1923 the ENM secured the Raadi manor, outside the center of the city, in a beautiful park, near the lake with its boats and water attractions. In this manor the first permanent exhibition of mainly 19th century Estonian peasant life was compiled, and the ENM operated in the Raadi manor from 1923–1940 as the “Estonian’s own museum” The years of alternating occupations, World War II, and political terror damaged and destroyed the whole society. The Raadi manor was destroyed in the war too, and the ENM itself was divided into two parts – the State Ethnographic Museum and the State Literary Museum. The collections of the ENM were given to Tallinn and to many different places inside and outside Tartu. Then began “the period of temporary location” that continues today. The museum is located in several places in the city of Tartu.In 1988, the prior name of the State Ethnographic Museum – the Estonian National Museum – was reinstated. Since the 1990s there have been many attempts to secure a special building for the ENM. Now, at last, as a result of serious economic pressure, we are closer to this goal than ever. During the last five

  12. Short outlines of books by Estonian authors / Brita Melts, Rutt Hinrikus and Janika Kronberg

    Index Scriptorium Estoniae

    Melts, Brita

    2015-01-01

    Arvustus: Viitol, Livia. Jutte ja novelle. Tallinn : Libri Livoniae, 2014 ; Talvet, Jüri. Ten Letters to Montaigne : "Self" and "other". Tartu : Tartu Ülikooli Kirjastus, 2014 ; Rooste, Jürgen. Suur sume, suur tume. [Luige (Kiili vald)] : Verb, 2014 ; Kross, Märten. Hullumäng. [Tallinn] : Tammerraamat, 2014 ; Kaus, Jan. Ma olen elus. Tallinn : Tuum, 2014 ; Kangro, Maarja. Hüppa tulle. Tallinn : Nähtamatu Ahv, 2014 ; Kasemaa, Andrus. Minu viimane raamat. Tallinn : Varrak, 2014 ; Raik, Katri. Minu Narva : kahe maailma vahel. [Tartu] : Petrone Print, 2013 ; Kareva, Doris. Maailma asemel. [Kordustr. Tallinn] : Verb, 2014

  13. Short outlines of books by Estonian authors / Brita Melts, Rutt Hinrikus and Janika Kronberg

    Index Scriptorium Estoniae

    Melts, Brita

    2012-01-01

    Arvustus: Kilusk, Ott. Veidrikud ja võpatused. Tallinn : Menu, 2012 ; Kilusk, Ott. Oktoobrikuine tuuker. Tallinn : Kirjastuskeskus, 2010 ; Hansen, Aime. Ma olin mereingel. Tallinn : Verb, 2011 ; Vallisoo, Mari. Koidutäht koolivihikus. Tartu : Ilmamaa, 2011 ; Sinijärv, Karl Martin. Krümitor 0671. Tallinn ; Näo Kirik, 2011 ; Aareleid, Kai. Vene veri. Tallinn : Varrak, 2011 ; Ashilevi, Jim. Ma olen elus olemise tunne. Luige : Verb, 2011 ; Traat, Mats. Vastsed Harala elulood. Tartu : Ilmamaa, 2011 ; Tamm, Margus. Unesnõiduja. Tallinn : Raudwara, 2011

  14. Development of genomic SSR and potential EST-SSR markers in ...

    African Journals Online (AJOL)

    In addition, forty four EST-SSRs which can be amplified with expected sizes were identified from a B. chinense root cDNA library. The genomic SSR markers and potential EST-SSR markers developed in the present study should be useful for genetic diversity and molecular marker assistant selection breeding research in ...

  15. Tallinn 2011 panustab tänavu turismifirmadesse / Kärt Anvelt, Jan Jõgis-Laats ; kommenteerib Roman Geiser

    Index Scriptorium Estoniae

    Anvelt, Kärt, 1973-

    2010-01-01

    Reklaamibüroo Zavod juht Marek Reinaas annab hinnangu SA Tallinn 2011 eelarvekava turundusosale. Kommunikatsioonispetsialist Daniel Vaarik viitab võimalusele kasutada Tallinn 2011 tutvustamiseks sotsiaalvõrgustikke, nt. Facebooki ja Twitterit

  16. The construction of gender in Estonian school textbooks / Jeannine Richards

    Index Scriptorium Estoniae

    Richards, Jeannine

    2001-01-01

    Uurimusest, mille eesmärgiks oli analüüsida nais- ja meessoo kujutamist inglise keele õpikus. Vaadeldavaks õpikuks on : Sotter, Ingrid ; Vahtra, Leida. English : step five. 5th print.- Tallinn : Koolibri, 1997

  17. Utilisation of Estonian energy wood resources

    Energy Technology Data Exchange (ETDEWEB)

    Muiste, P.; Tullus, H.; Uri, V. [Estonian Agricultural University, Tartu (Estonia)

    1996-12-31

    In the end of the Soviet period in the 1980s, a long-term energy programme for Estonia was worked out. The energy system was planned to be based on nuclear power and the share of domestic alternative sources of energy was low. The situation has greatly changed after the re-establishment of the Estonian independence, and now wood and peat fuels play an important role in the energy system. Energy consumption in Estonia decreased during the period 1970-1993, but this process has less influenced the consumption of domestic renewable fuels - peat and wood. It means that the share of these fuels has grown. The investment on substitution of imported fossil fuels and on conversion of boiler plants from fossil fuels to domestic fuels has reached the level of USD 100 million. The perspectives of the wood energy depend mainly on two factors; the resources and the price of wood energy compared with other fuels. The situation in wood market influences both the possible quantities and the price. It is typical that the quickly growing cost of labour power in Estonia is greatly affecting the price of energy wood. Though the price level of fuel peat and wood chips is lower than the world market price today, the conditions for using biofuels could be more favourable, if higher environmental fees were introduced. In conjunction with increasing utilisation of biofuels it is important to evaluate possible emissions or removal of greenhouse gases from Estonian forests 3 refs.

  18. Estonian greenhouse gas emissions inventory report

    Energy Technology Data Exchange (ETDEWEB)

    Punning, J.M.; Ilomets, M.; Karindi, A.; Mandre, M.; Reisner, V. [Inst. of Ecology, Tallinn (Estonia); Martins, A.; Pesur, A. [Inst. of Energy Research, Tallinn (Estonia); Roostalu, H.; Tullus, H. [Estonian Agricultural Univ., Tartu (Estonia)

    1996-07-01

    It is widely accepted that the increase of greenhouse gas concentrations in the atmosphere due to human activities would result in warming of the Earth`s surface. To examine this effect and better understand how the GHG increase in the atmosphere might change the climate in the future, how ecosystems and societies in different regions of the World should adapt to these changes, what must policymakers do for the mitigation of that effect, the worldwide project within the Framework Convention on Climate Change was generated by the initiative of United Nations. Estonia is one of more than 150 countries, which signed the Framework Convention on Climate Change at the United Nations Conference on Environment and Development held in Rio de Janeiro in June 1992. In 1994 a new project, Estonian Country Study was initiated within the US Country Studies Program. The project will help to compile the GHG inventory for Estonia, find contemporary trends to investigate the impact of climate change on the Estonian ecosystems and economy and to formulate national strategies for Estonia addressing to global climate change.

  19. Utilisation of Estonian energy wood resources

    International Nuclear Information System (INIS)

    Muiste, P.; Tullus, H.; Uri, V.

    1996-01-01

    In the end of the Soviet period in the 1980s, a long-term energy programme for Estonia was worked out. The energy system was planned to be based on nuclear power and the share of domestic alternative sources of energy was low. The situation has greatly changed after the re-establishment of the Estonian independence, and now wood and peat fuels play an important role in the energy system. Energy consumption in Estonia decreased during the period 1970-1993, but this process has less influenced the consumption of domestic renewable fuels - peat and wood. It means that the share of these fuels has grown. The investment on substitution of imported fossil fuels and on conversion of boiler plants from fossil fuels to domestic fuels has reached the level of USD 100 million. The perspectives of the wood energy depend mainly on two factors; the resources and the price of wood energy compared with other fuels. The situation in wood market influences both the possible quantities and the price. It is typical that the quickly growing cost of labour power in Estonia is greatly affecting the price of energy wood. Though the price level of fuel peat and wood chips is lower than the world market price today, the conditions for using biofuels could be more favourable, if higher environmental fees were introduced. In conjunction with increasing utilisation of biofuels it is important to evaluate possible emissions or removal of greenhouse gases from Estonian forests 3 refs

  20. Tallinn pürib Euroopa kultuuripealinnaks / Age-Kristel Kartau

    Index Scriptorium Estoniae

    Kartau, Age-Kristel

    2003-01-01

    Kultuuriväärtuste ameti ettevõtmisest, idekampaaniast juhuks, kui Tallinn peaks saama Euroopa kultuuripealinna tiitli aastal 2008. Artiklis ka eesti noorte filmirežissööride Hendrik Toompere jun., Marko Raadi ja Andri Luubi sõidust Inglismaale osalemaks "Tyne-Tallinn Digital Challenge" nimelises projektis

  1. Multi-Ethnicity and Material Exchangesin Late Medieval Tallinn

    DEFF Research Database (Denmark)

    Naum, Magdalena

    2014-01-01

    . It explores ambiguities arising from daily interactions in the shared physical landscape of the town, such as material exchanges, and the development of new technological solutions, and the simultaneous insistence on maintenance of sharp inter-group boundaries. As material culture plays a significant role......his article examines the cultural and social dynamics of a multi-ethnic medieval town. Taking the lower town of Tallinn as a case study, this paper identifies the major urban ethnic groups living in the town and discusses their co-existence, self-definition, and processes of categorization...

  2. Estonian Vodkat tuleb toota Eesti rukkist / Andres Reimer

    Index Scriptorium Estoniae

    Reimer, Andres

    2010-01-01

    Piiritusjookide määrusega Eesti geograafiliseks tähiseks saanud Estonian Vodka nimetusega viinad peaksid olema tehtud kodumaisest toorainest ning viinavabrikute ja piiritusetehase üle võiks toimuda kvaliteedikontroll

  3. Short outlines of books by Estonian authors / Berk Vaher, Aare Pilv

    Index Scriptorium Estoniae

    Vaher, Berk, 1975-

    2005-01-01

    Tutvustus: Raudam, Toomas. Nips. Tallinn : Eesti Keele Sihtasutus, 2004 ; Ilus Armin : eesti lühiproosat 1987-2002 / koost. Udo Uibo. Tallinn : Varrak, 2004 ; Ehlvest, Jüri von. Rahuldus. Tartu : Ilukirjandus, 2004 ; Ki:wa. Roboti tee on nihe ; Salatühik. Tallinn : Eesti Kunstimuuseum, 2004 ; Sommer, Lauri. Nõidade õrnus. Sännä : Tiivaalune, 2004 ; Kalkun, Andreas. Pääväraamat. Tartu : Erakkond, 2004 ; Jõerüüt, Jaak. Uus raamat. Tallinn : Tuum, 2004 ; Krull, Hasso. Meeter ja Demeeter. Tallinn : Vagabund, 2004 ; Kõiv, Madis. Päev. Tartu : Akkon, 2004 ; Mihkelson, Ene. Uroboros. Tallinn : Tuum, 2004 ; Soomets, Triin. Toormaterjal. Tallinn : Verb, 2004 ; Vaher, Berk. Sekeldaja päevad. Tallinn : Tuum, 2004

  4. Short outlines of books by Estonian authors / Brita Melts, Rutt Hinrikus and Janika Kronberg

    Index Scriptorium Estoniae

    Melts, Brita

    2015-01-01

    Arvustus: Afanasjev, Vahur. Tünsamäe tigu. Tartu Tartu NAK, 2015 ; Vadi, Urmas. Kuidas me kõik reas niimoodi läheme. Tallinn : Tuum, 2014 ; Traat, Mats. Kolm Solveigi. Tartu : Ilmamaa, 2015 ; Saat, Mari. Matused ja laulupeod. Tartu : Petrone Print, 2015 ; Kivisilla, Veronika. Cantus firmus. Tallinn : Näo Kirik, 2015 ; Kolk, Jüri. Suur võidujooks. Tallinn : Tuum, 2015 ; Mudlum. Tõsine inimene. Tallinn : ZA/UM, 2014

  5. SSR allelic variation in almond (Prunus dulcis Mill.).

    Science.gov (United States)

    Xie, Hua; Sui, Yi; Chang, Feng-Qi; Xu, Yong; Ma, Rong-Cai

    2006-01-01

    Sixteen SSR markers including eight EST-SSR and eight genomic SSRs were used for genetic diversity analysis of 23 Chinese and 15 international almond cultivars. EST- and genomic SSR markers previously reported in species of Prunus, mainly peach, proved to be useful for almond genetic analysis. DNA sequences of 117 alleles of six of the 16 SSR loci were analysed to reveal sequence variation among the 38 almond accessions. For the four SSR loci with AG/CT repeats, no insertions or deletions were observed in the flanking regions of the 98 alleles sequenced. Allelic size variation of these loci resulted exclusively from differences in the structures of repeat motifs, which involved interruptions or occurrences of new motif repeats in addition to varying number of AG/CT repeats. Some alleles had a high number of uninterrupted repeat motifs, indicating that SSR mutational patterns differ among alleles at a given SSR locus within the almond species. Allelic homoplasy was observed in the SSR loci because of base substitutions, interruptions or compound repeat motifs. Substitutions in the repeat regions were found at two SSR loci, suggesting that point mutations operate on SSRs and hinder the further SSR expansion by introducing repeat interruptions to stabilize SSR loci. Furthermore, it was shown that some potential point mutations in the flanking regions are linked with new SSR repeat motif variation in almond and peach.

  6. Estonian Airi Boeing sattus kaks korda hädaolukorda / Krister Kivi

    Index Scriptorium Estoniae

    Kivi, Krister, 1975-

    2011-01-01

    Estonian Airi Boeing ES-ABL sattus 2011. aasta algus kahel korral ohtlikku olukorda. Lennuamet tegi Estonian Airile ettekirjutuse, kuid seda pole ameti kodulehel avaldatud. Lennufirma kapteni ja lennuohutuse inspektori Andreas Kari, lennuameti selgitusi

  7. Estonian Air valmistub odavate piletitega EasyJeti tulekuks / Erkki Erilaid

    Index Scriptorium Estoniae

    Erilaid, Erkki

    2004-01-01

    Estonian Air pidi langetama piletihindu Berliini ja Londoni liinil kolmandiku võrra, kuna oktoobri lõpust hakkab Tallinna lennujaamast reise tegema uus odavlennufirma Easy-Jet. Lisa: Estonian Airi hinnad internetis

  8. Short Outlines of Books by Estonian Authors / Janika Kronberg, Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2009-01-01

    Arvustus: Kaplinski, Jaan. Parallelle ja parallelisme. Tartu : Tartu Ülikooli Kirjastus, c2009 ; Hellat, Henn-Kaarel. Inimese tegemine I-V. Tallinn : Faatum, 2002-2008 ; Nõu, Helga. Elu täis üllatusi. Tartu : Atlex, 2009 ; Remsu, Olev. Musketäride muundumised. [Tallinn] : Eesti Keele Sihtasutus, 2008 ; Ristikivi, Karl. Päevaraamat 1957-1968. Tallinn : Varrak, 2008

  9. Energy potential of the Estonian rivers

    International Nuclear Information System (INIS)

    Reihan, Alvina; Kovalenko, Olga

    2002-01-01

    In the current study the river runoff, its long-term distribution and river slope were considered as factors in the evaluation of the hydro energy potential of the Estonian rivers. The analyses of the long-term runoff hydrographs showed the increase of the maximum discharges and the decrease of the minimum discharges, at the same time mean annual discharges were almost unchanged. It indicated that seasonal variability of the runoff decreased. The evaluation of the hydro energy potential of rivers showed, that the Narva and Paernu rivers are the most suitable for hydro energy production, then in decreasing order - the Kasari, Pedja, Piusa, Halliste, Vohandu, Jaegala rivers etc. The analyses of the Rannapungerja river runoff showed, that the activity of the restored Tudulinna hydropower plant had an influence on runoff in 2001: the minimum discharge was less than permitted by the legislative acts. Thus, the environmental aspects should have a high priority in hydropower plants reconstruction(author)

  10. simple sequence repeats (EST-SSR)

    African Journals Online (AJOL)

    Yomi

    2012-01-19

    Jan 19, 2012 ... 212 primer pairs selected, based on repeat patterns of n≥8 for di-, tri-, tetra- and penta-nucleotide repeat ... Cluster analysis revealed a high genetic similarity among the sugarcane (Saccharum spp.) breeding lines which could reduce the genetic gain in ..... The multiple allele characteristic of SSR com-.

  11. Chromosomal location of genomic SSR markers associated

    Indian Academy of Sciences (India)

    We have previously reported Xgwm382 as a diagnostic marker for disease resistance against yellow rust in Izgi2001 × ES14 F2 population. Among the same earlier tested 230 primers, one SSR marker (Xgwm311) also amplified a fragment which is present in the resistant parent and in the resistant bulks, but absent in the ...

  12. (SSR) and inter simple sequence repeat (ISSR)

    African Journals Online (AJOL)

    Finally, they were washed 3 to 4 times with sterile distilled water and inoculated aseptically on Murashige and Skoog (MS) basal medium free hormones. Single nodes resulted from seedlings cultured as explants. Inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) primers used produced different ...

  13. Joakim Helenius: Estonian Air võtku eeskuju airBalticust / Siim Sultson

    Index Scriptorium Estoniae

    Sultson, Siim

    2010-01-01

    Estonian Airi ühe võimaliku nõukogu esimehe Jaokim Heleniuse hinnangul on väikeste lennufirmade, nagu Estonian Air ja airBaltic jaoks päris palju tegutsemisruumi. Estonian Airil tuleb leida oma nišš ja kindel, kuid omanäoline strateegia

  14. Adapting the Survey of Attitudes towards Statistics (SATS-36) for Estonian Secondary School Students

    Science.gov (United States)

    Hommik, Carita; Luik, Piret

    2017-01-01

    The purpose of this study is to adapt the Survey of Attitudes Towards Statistics (SATS-36) for Estonian secondary school students in order to develop a valid instrument to measure students' attitudes within the Estonian educational context. The SATS-36 was administered to Estonian-speaking secondary school students before their compulsory…

  15. Short outlines of books by Estonian authors / Janika Kronberg and Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2010-01-01

    Arvustus: Kiin, Sirje. Marie Under : elu, luuletaja identiteet ja teoste vastuvõtt. Tallinn : Tänapäev, 2009 ; Laanes, Eneken. Lepitamatud dialoogid : subjekt ja mälu nõukogudejärgses eesti romaanis. Tartu : Tartu Ülikooli Kirjastus, 2009 ; Ehin, Kristiina. Emapuhkus. Tallinn : Pandekt, 2009 ; Tungal, Leelo. Seltsimees laps ja suured inimesed. Tallinn : Tänapäev, 2008 ; Tungal, Leelo. Samet ja saepuru, ehk, Seltsimees laps ja kirjatähed. Tallinn : Tänapäev, 2009 ; Õnnepalu, Tõnu. Paradiis. Tallinn : Varrak, 2009

  16. Short outlines of books by Estonian authors / Janika Kronberg, Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2006-01-01

    Arvustus: Runnel, Hando. Sinamu. Tartu : Ilmamaa, 2005 ; Talvet, Jüri. Tõrjumatu äär. Tartu : Ilmamaa, 2005 ; Nõu, Enn. Mõtusekuke viimne kogupauk. Tallinn : Eesti Keele Sihtasutus, 2005 ; Merca. Hele häärber. Tartu : Vilep & Vallik, 2005 ; Õnnepalu, Tõnu. Enne heinaaega ja hiljem : luulet ja luuletõlkeid 1983-2005. Tallinn : Eesti Keele Sihtasutus, 2005 ; Kaplinski, Jaan. Kõik on ime. Tartu : Ilmamaa, 2004 ; Unt, Mati. Vend Antigone, ema Oidipus. Tallinn : Kultuurileht, 2006 ; Laretei, Käbi. Otsekui tõlkes : teema variatsioonidega. Tallinn : Tänapäev, 2005 ; Jõerüüt, Jaak. Sõber : valik novelle 1979-2005. Tallinn : Tuum, 2005

  17. Short outlines of books by Estonian authors / Janika Kronberg, Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2005-01-01

    Tutvustus: Henno, Sass. Mina olin siin. 1 : esimene arest. Tallinn : Eesti Päevaleht, 2005 ; Kruusvall, Jaan. Sinetavad kaugused : valitud proosat. Tallinn : Eesti Raamat, 2004 ; Hirv, Indrek. Klaaskübara all : [esseed]. Tartu : Ilmamaa, 2004 ; Õudne Eesti : valimik eesti õudusjutte. Tallinn : Varrak, 2005 ; Ruitlane, Olavi. Kroonu. Räpina : Väiku Välläandja, 2005 ; Ehin, Kristiina. Kaitseala. Tallinn : Huma, 2005 ; Kivirähk, Andrus. Jutud. Tallinn : Eesti Kirjanduse Sihtasutus, 2005 ; Talvet, Jüri. Unest, lumest. Tartu : Ilmamaa, 2005 ; Künnap, Asko. Kõige ilusam sõda. Tallinn : Näo Kirik, 2004 ; Kaplinski, Jaan. Kaks päikest : teistmoodi muinaslood. Tallinn : Tänapäev, 2005 ; Alver, Betti. Koguja : suur luuleraamat. Tartu : Ilmamaa, 2005

  18. Estonian Airi president : uus äristrateegia toob ettevõttele edu / Borge Thornbech ; interv. Andres Reimer

    Index Scriptorium Estoniae

    Thornbech, Borge

    2007-01-01

    Estonian Air asutas regionaalsete lendude korraldamiseks ettevõtte Estonian Air Regional, idee on arendada tegevust lühidistantsidel ja luua ühenduslüli lennukompanii Euroopa-liinide vahel. Kommenteerivad Olev Schults, Oleg Harlamov, Rein Mark. Vt. samas: Kõik aktsionärid vannuvad Estonian Airile truudust; Estonian Air kaalub Tartu lennuliini avamist. Kaart: Reisijate jagunemine sihtkohtade vahel. Graafikud: Estonian Air kukkus kahjumisse

  19. Siim Nestor soovitab : Tallinn Doom Night vol.1. Odessa Pop / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2006-01-01

    Soome doom-metal ansambel Reverend Bizarre üritusel "Tallinn Doom Night vol.1" 8. dets. klubis Rockstar's. Indipoppi viljelev Rootsi duo My Darling Yoy! üritusel "Odessa Pop" 9. dets. Tallinnas klubis KuKu

  20. Uhiuus orkester Tallinn Sinfonietta / Ivo Lille ja Risto Joost ; intervjueerinud Kristina Kõrver

    Index Scriptorium Estoniae

    Lille, Ivo, 1977-

    2009-01-01

    Kontserdiagentuurist HIMusic Agency ja Tallinn Sinfoniettast räägivad dirigent R. Joost ja I. Lille. Avakontserdist Vene Kultuurikeskuses, kus oli kavas Mozarti sümfooniad 1, 10 ja 40 ning kontsertaariad bulgaaria soprani Sonya Youncheva esituses

  1. [Karin Hallas. Das Tallinner Mietshaus. Vom Historismus bis zum Jugendstil] / Paul Kaegbein

    Index Scriptorium Estoniae

    Kaegbein, Paul

    2006-01-01

    Arvustus: Karin Hallas. Das Tallinner Mietshaus. Vom Historismus bis zum Jugendstil. In: Architektur und bildende Kunst im Baltikum um 1900. Frankfurt am Main, Berlin, Bern, Bruxelles, New York, Wien: Lang 1999. S. 173-192.

  2. Tallinn 2011 ... mitte ainult koorikonkurss / Aivar Leštšinski

    Index Scriptorium Estoniae

    Leštšinski, Aivar, 1958-

    2011-01-01

    Festivali avapäeval esitletud CD-komplektist "Läbilõige eesti koorimuusikast" ja mõtteid, et riigitelevisioonis võik olla koorimuusikat puudutav saade ning koorifestivali "Tallinn 2011" tulemused

  3. Euroopa kultuuripealik 2011 (Tallinn) lõpuks leitud / Mikko Fritze ; interv. Kaarel Tarand

    Index Scriptorium Estoniae

    Fritze, Mikko, 1963-

    2007-01-01

    Tallinn on Euroopa 2011. a. kultuuripealinn. Intervjuu korraldava sihtasutuse tegevjuhi Mikko Fritzega. Tegevusprogrammist, ka infrastruktuuri projektidest (linnateater, Kultuurikatel, rannapromenaad, lauluväljak, linnahall) jm. SA bürool on kavatsus kolida Kultuurikatlasse

  4. "Tallinn Jazz Weekend" tõi kultuuripealinna Euroopa jazzielu korraldajad / Marje Ingel, Joosep Sang

    Index Scriptorium Estoniae

    Ingel, Marje

    2011-01-01

    Jazziorganisatsioonist Europe Jazz Network ja selle aastakonverentsist Tallinnas ning J. Sang selle raames Von Krahli teatris toimunud Tallinn Jazz Weekendi eesti jazzmuusika showcase-festivalist lühidalt

  5. The Role of Accountant in the Estonian Enterprise

    Directory of Open Access Journals (Sweden)

    Ergo Ottoson

    2016-06-01

    Full Text Available Recent developments and changes in the modern economy as well as falls and rises of large corporations have brought up the public interest towards the accounting discipline, and have resulted in a growing demand for accountant specialists as educated professionals. The aim of the present research is to find out what is the role of the accountant in the modern enterprise. The authors conducted a survey among Estonian accounting specialists and managers. The results of the survey clearly indicate that in most Estonian companies, accountants are actively assisting members of the board to make vital decisions, and their competence, knowledge and skills are highly valued by managers. The findings of the survey emphasize that Estonian accountants are highly qualified, keeping in line with the latest developments in the modern financial world.

  6. Arduino Based RFID Line Switching Using SSR

    Directory of Open Access Journals (Sweden)

    Michael E.

    2017-10-01

    Full Text Available The importance of line switching cannot be overemphasized as they are used to connect and disconnect substations to and from a distribution grid. At the cradle of technology line switching was achieved via the use of manual switches or fuses which could endanger life as a result of electrocution when expose during maintenance. This ill prompted the development of automated line switching using relays and contactors. With time this tends to fail as a result of wearing of the contact which is as a result of arcing and low voltage. To avert all these ills this paper presents Arduino based Radio Frequency Identification RFID line switching using Solid State Relay SSR. This is to ensure the safety of operators or technologist and to also avert the problem associated with relays and contactors using SSR. This was achieved using RFID RC-522 reader ardriuno Uno SSR and other discrete components. The system was tested and worked perfectly reducing the risk of electrocution and eliminating damage wearing of the contacts common with contactors and relays.

  7. Ethnic Self-Esteem and Intergroup Attitudes Among the Estonian Majority and the non-Estonian Minority

    Directory of Open Access Journals (Sweden)

    Maaris Raudsepp

    2009-11-01

    Full Text Available The study was focussed on the relationships between ethnic self-esteem and various indicators of intergroup attitudes in a representative sample of adult population of Estonia (N=1142. Attitudinal variables that discriminated most between persons with high and low ethnic self-esteem were identified. Among Estonians ethnic self-esteem was related to positive ingroup bias, readiness for outgroup contact, perceived threat from the outgroup, attitudes to non-Estonian minority, and attitudes toward minority integration. Among non-Estonians ethnic self-esteem was related to readiness for outgroup contact, ethnic sterotypes, and various attitudes towards minority integration. An attempt was made to reconstruct the system of intergroup attidues of prototypical persons with high and low ethnic selfesteem and to describe psychological implications of high and low ethnic self-esteem for members of majority and minority groups. Various theoretical models (social identity theory, integrated threat theory, social dominane theory were used for interpretation of the results.

  8. Short outlines of books by Estonian authors / Janika Kronberg, Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2003-01-01

    Talvet, Jüri. ElegÍa Estonia y otros poemas = Eesti eleegia ja teisi luuletusi / Spanish transl. by the author and Albert L̉zaro Tinaut. Ciudad de València : Palmart, 2002 ; Ehlvest, Jüri. Hobune eikuskilt. Tallinn : Tänapäev, 2002 ; Raudam, Toomas. Saint-Prousti vastu. Tallinn : Eesti Keele Sihtasutus, 2002 ; Tode, Emil. Raadio. Tallinn : Eesti Keele Sihtasutus, 2002 ; Vene, Ilmar. Pahustumine ehk Uusaja olemus. Tallinn : Eesti Keele Sihtasutus, 2002 ; Annus, Epp. Kuidas kirjutada aega. Tallinn : Underi ja Tuglase Kirjanduskeskus, 2002 ; Lepik, Kalju. Valguse riie ei vanu. Kogutud luuletused 1938-1999 ; Barker, Matt. Waanenburgi tapatalgud. Tartu : EYS Veljesto, 2002 ; Kesküla, Kalev. Platoni riik. Tallinn : Tuum, 2002 ; Vahing, Vaino. Mängud ja kõnelused. Tallinn : Eesti Keele Sihtasutus, 2002 ; Tuulik, Jüri. Üksik lind mere kohal. Tallinn : Maalehe Raamat, 2002 ; Tätte, Jaan. Näidendid. Tallinn : Maalehe Raamat, 2002 ; Kareva, Doris. Mandragora. Tallinn : Huma, 2002

  9. Short outlines of books by Estonian authors / Leena Kurvet-Käosaar, Brita Melts, Rutt Hinrikus and Janika Kronberg

    Index Scriptorium Estoniae

    2011-01-01

    Arvustus: Nurklik, Siim. Kas ma olen nüüd elus. Tallinn : S. Nurklik, 2010 ; Lintrop, Aado. Annapurna. Tartu : Ilmamaa, 2010 ; Lintrop, Aado. Õhtud sõidavad õue. Tartu : Ilmamaa, 2011 ; Saar, Anti. Tekste siledast ruumist. Tallinn : Eesti Keele Sihtasutus, 2009 ; Hvostov, Andrei. Sillamäe passioon. Tartu : Petrone Print, 2011 ; Nõu, Helga. Valetaja. Tallinn : Eesti Ajalehed, 2011 ; Malin, Jaan. Alati vahe. Tallinn : Tuum, 2010 ; Jõerüüt, Jaak. Muutlik. Tallinn : Tuum, 2010 ; Luik, Viivi. Varjuteater. Tallinn : Eesti Keele Sihtasutus, 2010

  10. Data Envelopment Analysis of Helsinki-Tallinn Transportation Chains

    Directory of Open Access Journals (Sweden)

    Olli-Pekka Hilmola

    2013-12-01

    Full Text Available The Baltic Sea shipping is at a crossroads as sulphur regulation will lead to excessive cost increases from the year 2015 onwards and CO2 emission trading is planned to be implemented for the entire shipping sector within the EU area. Therefore, shipping is going to be minimized and hinterland transportation (road and rail will act as substitute. This research analyzes the situation on one of the highest volume general cargo transportation routes of Finland (operating between Helsinki, Finland and Tallinn, Estonia, including loading and unloading at seaports and short sea shipping activity in between. Based on the efficiency evaluation results, it seems that containers should be favoured over semi-trailers – containers could be carried efficiently either in container ships or even at currently favoured RoRo or RoPax ships. Our research illustrates that pure container shipping with larger container ships within the analyzed route is not entirely out of question, but lead time and hinterland operations should receive more attention. Alternatively, RoRo and RoPax ships can also do something to increase their competitiveness in environmental harm caused and diesel consumption – higher cargo loads and utilization levels are short-term key for continued dominance.

  11. Personal control of privacy and data: Estonian experience.

    Science.gov (United States)

    Priisalu, Jaan; Ottis, Rain

    2017-01-01

    The Republic of Estonia leads Europe in the provision of public digital services. The national communications and transactions platform allows for twenty-first century governance by allowing for transparency, e-safety (inter alia privacy), e-security, entrepreneurship and, among other things, rising levels of prosperity, and well-being for all its Citizens. However, a series of Information Infrastructure attacks against the Estonian e-society infrastructure in 2007 became one of best known incidents and experiences that fundamentally changed both Estonian and international discussions about Cyber Security and Privacy. Estonian experience shows that an open and transparent attitude provides a good foundation for trust between the Citizen and the State, and gives more control to the real owner of the data - the Citizen. Another important lesson is that the Citizen needs to be confident in the government's ability to keep their data safe -- in terms of confidentiality, integrity and availability - establishing a strong link between privacy and information security. This paper discusses certain critical choices, context, and events connected to the birth and growth of the Estonian e-society in terms of Privacy.

  12. Kas Bombardier lennutab Estonian Airi üles? / Henrik Ilves

    Index Scriptorium Estoniae

    Ilves, Henrik

    2011-01-01

    Estonian Air on saanud oma käsutusse kaks kolmest ostetud lennukist Bombardier CRJ900, millega saavutatakse Boeing 737-ga võrreldes oluline kütuse kokkuhoid. 20 aastaga on müüdud CRJ-seeria lennukeid juba üle 1700

  13. Estonian Golf & Country Club / Liina Jänes

    Index Scriptorium Estoniae

    Jänes, Liina, 1977-

    2005-01-01

    Estonian Golf & Country Club'i etnomodernistlik golfikeskus ja klubihoone Jõelähtmel. Projekteerija: Arhitektuuristuudio Siim & Kreis. Autor Andres Siim. Konstruktor: Resand. Sisekujundaja Juta Lember (SAB Lember & Padar). Projekt 2004, valmis 2005. Ill.: I ja II korruse plaan, 3 värv. välis ja 3 sisevaadet

  14. Estonian Golf & Country Clubi klubihoone / Andres Siim, Alar Just

    Index Scriptorium Estoniae

    Siim, Andres

    2005-01-01

    Harjumaal Jõelähtme vallas rajab Estonian Golf & Country Club uut Jägala-Jõesuu spordi- ja puhkekeskust, mille südameks saab puidust golgiklubi hoone, mida tutvustavad klubihoone arhitekt ja üks inseneridest. Ill.: vaade ehitusele, projekti kaks vaadet, lõige

  15. Top 10 Estonian albums of 2004 / Igor Garshnek

    Index Scriptorium Estoniae

    Garšnek, Igor, 1958-

    2004-01-01

    Heliplaatidest: Arvo Pärt "Pro et contra", Various composers "Baltic Voices 2", Raimo Kangro "Displays", Toivo Tulev "Be Lost in the Call", Indrek Vau and Mati Mikalai "Estonian Trumpet Music", Erdmann/Sooäär "Dessert Time, Peer Gynt & Other Stories", Alo Mattiisen "50 parimat laulu", Riho Sibul "Must", Rein Rannap "Tantsib klaveril", Eesti Keeled "Kella tiksumist..."

  16. Organizational Commitment in Estonian University Libraries: A Review and Survey

    Science.gov (United States)

    Kont, Kate-Riin; Jantson, Signe

    2014-01-01

    The data used in this article is based on the reviewing of relevant literature to provide an overview of the concepts of organizational commitment, job security, and interpersonal relations, as well as on the results of the original online survey, conducted by the article's authors, held in 2012 in Estonian university libraries governed by public…

  17. SSR and EST-SSR-based genetic linkage map of cassava (Manihot esculenta Crantz).

    Science.gov (United States)

    Sraphet, Supajit; Boonchanawiwat, Athipong; Thanyasiriwat, Thanwanit; Boonseng, Opas; Tabata, Satoshi; Sasamoto, Shigemi; Shirasawa, Kenta; Isobe, Sachiko; Lightfoot, David A; Tangphatsornruang, Sithichoke; Triwitayakorn, Kanokporn

    2011-04-01

    Simple sequence repeat (SSR) markers provide a powerful tool for genetic linkage map construction that can be applied for identification of quantitative trait loci (QTL). In this study, a total of 640 new SSR markers were developed from an enriched genomic DNA library of the cassava variety 'Huay Bong 60' and 1,500 novel expressed sequence tag-simple sequence repeat (EST-SSR) loci were developed from the Genbank database. To construct a genetic linkage map of cassava, a 100 F(1) line mapping population was developed from the cross Huay Bong 60 by 'Hanatee'. Polymorphism screening between the parental lines revealed that 199 SSRs and 168 EST-SSRs were identified as novel polymorphic markers. Combining with previously developed SSRs, we report a linkage map consisted of 510 markers encompassing 1,420.3 cM, distributed on 23 linkage groups with a mean distance between markers of 4.54 cM. Comparison analysis of the SSR order on the cassava linkage map and the cassava genome sequences allowed us to locate 284 scaffolds on the genetic map. Although the number of linkage groups reported here revealed that this F(1) genetic linkage map is not yet a saturated map, it encompassed around 88% of the cassava genome indicating that the map was almost complete. Therefore, sufficient markers now exist to encompass most of the genomes and efficiently map traits in cassava.

  18. Short outlines of books by Estonian authors / Janika Kronberg, Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2003-01-01

    Sinijärv, Karl- Martin. Artutart & 39. Tallinn : Tuum, 2002 ; Beier, Priidu. Monaco. Tartu : P. Beier, 2002 ; Malin, Jaan. Sulle. Tartu : J. Malin, 2003 ; Liiv, Toomas. Luuletused 1968-2002. Tallinn : Tuum, 2003 ; Mägi, Arvo. Katk ja koolera. Tartu : Ilmamaa ; Kross, Jaan. Omaeluloolisus ja alltekst. Tallinn : Eesti Keele Sihtasutus, 2003 ; Kaplinski, Jaan ; Salminen, Johannes. Ööd valged ja mustad. Tallinn : Loomingu Raamatukogu, 2003 ; Kauksi Ülle. Käänüpäiv. Tartu : Kauksi Ülle Mänedsment', 2003 ; Luik, Tõnu. Filosoofiast kõnelda. Tartu : Ilmamaa, 2002 ; Teder, Tarmo. Pööningujutud. Tallinn : Eesti Keele Sihtasutus, 2002 ; Vetemaa, Enn. Minu väga magus elu ehk martsipanimeister. Tallinn : Tänapäev, 2002 ; Põldroos, Enn. Joonik kivi. Tallinn : Eesti Keele Sihtasutus, 2003 ; Suburg, Lilli. Kogutud kirjatööd. Tallinn : Eesti Raamat, 2002 ; Mikelsaar, Henn. Müüritud. Tartu : Ilmamaa, 2003 ; Kaus, Jan. Õndsate tund. Tallinn : Tuum, c2003

  19. SSR markers reveal genetic variation between improved cassava ...

    African Journals Online (AJOL)

    SERVER

    2007-12-03

    Dec 3, 2007 ... Afr. J. Biotechnol. 4(9): 873-881. Mba REC, Stephenson P, Edwards K, Melzer S, Mkumbira J, Gullberg. U. Apel K, Gale M, Tohme J, Fregene M (2000). Simple Sequence. Repeats (SSR) Markers Survey of the Cassava (Manihot esculenta. Crantz) genome: Towards an SSR-based molecular genetic map of.

  20. SSR markers reveal genetic variation between improved cassava ...

    African Journals Online (AJOL)

    ranged from 0.42 to 0.84, and 12 distinct DNA cluster groups were identified at 0.70 coefficients using Numerical Taxonomy and Multivariate Analysis System software package. For the genotype identification study, the 16 SSR primers were screened by their polymorphic information content (PIC) values. Five SSR primers ...

  1. An online conserved SSR discovery through cross-species comparison

    Directory of Open Access Journals (Sweden)

    Tun-Wen Pai

    2009-02-01

    Full Text Available Tun-Wen Pai1, Chien-Ming Chen1, Meng-Chang Hsiao1, Ronshan Cheng2, Wen-Shyong Tzou3, Chin-Hua Hu31Department of Computer Science and Engineering; 2Department of Aquaculture, 3Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan, Republic of ChinaAbstract: Simple sequence repeats (SSRs play important roles in gene regulation and genome evolution. Although there exist several online resources for SSR mining, most of them only extract general SSR patterns without providing functional information. Here, an online search tool, CG-SSR (Comparative Genomics SSR discovery, has been developed for discovering potential functional SSRs from vertebrate genomes through cross-species comparison. In addition to revealing SSR candidates in conserved regions among various species, it also combines accurate coordinate and functional genomics information. CG-SSR is the first comprehensive and efficient online tool for conserved SSR discovery.Keywords: microsatellites, genome, comparative genomics, functional SSR, gene ontology, conserved region

  2. Eesti Draamateatri interjööride renoveerimine : Pärnu mnt. 5, Tallinn = Renovation of the interior of the Estonian Drama Theatre : Pärnu mnt. 5, Tallinn / Epp Lankots

    Index Scriptorium Estoniae

    Lankots, Epp, 1976-

    2005-01-01

    Teatrihoone uus sisekujundus koostrati suuremahuliste rekonstrueerimistööde käigus. Sisearhitekt Mari Kurismaa pälvis Eesti Draamateatri renoveerimise eest Eesti Kultuurkapitali arhitektuuri sihtkapitali 2004. a. renoveerimispreemia. Autoritöö sulandub renoveerimisel algupärase interjööriga. Ill.: 1 värv. välis- ja 8 sisevaadet

  3. CLUPEA HARENGUS MEMBRAS: ABOUT THE ETYMOLOGY OF A CERTAIN FISH NAME IN ESTONIAN, LATVIAN AND LIVONIAN

    Directory of Open Access Journals (Sweden)

    Udo Uibo

    2012-01-01

    Full Text Available In this article, the names of Clupea harengus membras will be considered in Estonian, (Salaca Livonian and Latvian (respectively räim, reńǵ and reņģe ‘Baltic herring’. It will be shown that the source of all of these words is the Estonian-Swedish strämg (sträηg, or its preceding word shape *sträimg. The Latvian reņģe is borrowed from Estonian-Swedish through Salaca Livonian. Paul Ariste proposed an adequate etymology for the Estonian räim as early as 1933.

  4. [Pärtel Lippus. The acoustic features and perception of the Estonian quantity system] / Stefan Werner

    Index Scriptorium Estoniae

    Werner, Stefan

    2012-01-01

    Arvustus: Pärtel Lippus. The acoustic features and perception of the Estonian quantity system. Tartu : Tartu University Press, 2011. (Dissertationes philologiae estonicae Universitatis Tartuensis ; 29)

  5. Estonian Air püüdis viimase hetkeni Top Toursi päästa / Mirko Ojakivi

    Index Scriptorium Estoniae

    Ojakivi, Mirko

    2010-01-01

    Estonian Air kaalus Top Toursile appiminekut, sest reisifirma oli Estonian Airil üks olulisemaid koostööpartnereid. Abi oleks puudutanud ennekõike uutele lendudele allahindluse tegemist, räägiti ka võimalikust krediidist

  6. Eesti Lastekirjanduse Keskus = The Estonian Children's Literature Centre

    Index Scriptorium Estoniae

    2008-01-01

    Eesti Lastekirjanduse Keskuse hoone (1910, Pikk 73, Tallinn) sisekujundusest. Sisearhitektid: Malle Jürgenson, Krista Lepland ja Tea Tammelaan (Laika, Belka & Strelka OÜ). Varakambrit kaunistavad istmed erinevate illustraatorite tehtud maalingutega. Muinasjututoa aknaluuke kaunistavad Jüri Mildebergi loodud fantastilised tüübid. Pööningukorrust kujundas Piret Mildeberg. Sisearhitektidest, nende tähtsamad ühiselt tehtud tööd. 3 korruse plaani, värv. välisvaade, 9 sisevaadet, fotod sisearhitektidest

  7. Iseseisvusdeklaratsioonid 1776–1918. The Estonian Declaration of Sovereignty: An Example of the Civilizing Force of Hypocrisy

    Directory of Open Access Journals (Sweden)

    Hent Kalmo

    2012-06-01

    proposed amendments to the Soviet Constitution (that would have curbed the rights of the Union Republics by adopting a Declaration of Sovereignty, which effectively made the application of Soviet legal acts optional in the terrirory of the Estonian SSR. The Soviet leadership reacted in a confused manner, as it first responded positively to the Declaration, then condemned it as illegal, but also admitted that it was an expression of legitimate concerns. The fact that the notion of sovereignty was an important part of the orthodox Soviet constitutional doctrine, and the apprehension that the Estonian interpretative position might diffuse into other Union Republics, induced the Politburo to orchestrate a highly publicised denunciation of the Declaration. But this had the paradoxical effect of casting the Declaration as something that could be discussed, i.e. as something that deserved an argued rebuttal. In a way, the Politburo thus contributed to making the Estonian Declaration into a model that was later copied by almost all the Union Republics as they started either to head towards independence or jockey for a better position in the negotiations over the new Union Treaty, drafted in haste by the part of the Soviet leadership that was willing to opt for a confederate solution. The debate over the nature of Soviet federalism and its political aftermath illustrates the fact that a constitution which lies dormant under an authoritarian regime as a document of cynical hypocrisy has the potential to become a focal point for the formulation of political demands when circumstances change. The options open to the Soviet leadership in responding to the steps taken by the Baltic independence movements were clearly influenced, at least to some extent, by the vocabulary it was able to draw on to justify its response, especially since it had recently vowed to take the Soviet Constitution seriously in the future. Under propitious circumstances, its own hypocrisy could indeed exert a

  8. Critical Success Factors and information needs in Estonian industry

    Directory of Open Access Journals (Sweden)

    Aiki Tibar

    2002-01-01

    Full Text Available The article reports the results of the study on the critical success factors and related information needs in Estonian industry conducted in 1999. Data were collected by interviews with 27 managers and engineers from 16 manufacturing companies in various industries. Most of the critical success factors taken up were related to marketing, information management, quality management, product development and technological innovations. The information needs of managers and engineers were related to competitors, customers, markets, technology, regulations, etc. Some identified CSFs expressed also priorities for development by Estonian economic authorities: to support the implementation of new technologies and introduction of quality management methods. The finding that information management was perceived as a very critical area supports the result of the recent Finnish study on CSFs.

  9. Rupturing otherness: becoming Estonian in the context of contemporary Britain.

    Science.gov (United States)

    Märtsin, Mariann

    2010-03-01

    While identity construction continues to be a widely discussed and researched area in contemporary social sciences, the existing theories have overlooked the importance of understanding why and how identities as semiotic constructions emerge in individuals' consciousness in the flow of their everyday functioning. This article seeks to address this limitation in the theorizing by proposing an alternative conceptualization of identity, according to which identity construction is triggered by rupturing life-experience, which surfaces another perspective and makes the person aware of a possibility to be otherwise or of the reality of being different. Theoretical claims put forward in the paper are drawn from data gathered in a recent study, which explored lived-through experiences of young Estonians, who made study-visits to the United Kingdom. The discussed data will also highlight some interesting aspects in Estonians' self-definition as it is constructed in relation to Eastern-European identity in the context of contemporary Britain.

  10. Püha Siimeoni ja Naisprohvet Hanna kiriku restaureerimine : Ahtri 5, Tallinn = Restoration of the Church of Righteous Symeon and Prophetess Ann : Ahtri 5, Tallinn / Epp Lankots

    Index Scriptorium Estoniae

    Lankots, Epp, 1976-

    2005-01-01

    18. sajandil ehitatud, 2001. a. Eesti Apostlik-Õigeusu Kirikule tagastatud kirikuhoone restaureerimine. Ees on interjööride konserveerimistööd. Uuringud: Juhan Kilumets, Eva Mölder. Arhitektuurne projekt: Tõnu Parmakson, Ilmar Kannelmäe. Konstruktiivne projekt: Aleksander Danil. Teostus: J. Kilumets (Rändmeister), T. Parmakson (Frantsiskus), E. Mölder (Vana Tallinn). Ill.: 3 värv. välis- ja 3 sisevaadet

  11. Estonian Leader's Freedom Call Creates Storm / Anna Smolchenko

    Index Scriptorium Estoniae

    Smolchenko, Anna

    2008-01-01

    President Toomas Hendrik Ilvese esinemisest soome-ugri rahvaste maailmakongressil Hantõ-Mansiiskis, kohtumisest Venemaa presidendi Dmitri Medvedeviga, Eesti delegatsiooni saalist väljamarssimisest Venemaa riigiduuma väliskomisjoni esimehe Konstantin Kossatshovi sõnavõtu ajal. Ilmunud ka: St. Petersburg Times 1. juuli 2008, pealk.: Estonian Leader's Freedom Call Creates Controversy (lüh.). Vabariigi President töövisiidil Venemaal 27.-30.06.2008

  12. Exhibition of photography from the Estonian diaspora / Ellu Maar

    Index Scriptorium Estoniae

    Maar, Ellu, 1982-

    2010-01-01

    Näitus "Photography from the Estonian Diaspora / Väliseesti foto" Kumu Kunstimuuseumis 8.10.-19.11.2010, kuraatorid Eha Komissarov ja Ellu Maar. Näitus tutvustas 1944. a. Eestist lahkunud või juba võõrsil sündinud fotograafide (Eric Soovere, Karl Hintzer, Priit Vesilind, Rein Välme jt.) loomingut ja valikut väliseesti fotoarhiividest

  13. The Role of Accountant in the Estonian Enterprise

    OpenAIRE

    Ergo Ottoson; Monika Nikitina-Kalamae; Natalja Gurvitš

    2016-01-01

    Recent developments and changes in the modern economy as well as falls and rises of large corporations have brought up the public interest towards the accounting discipline, and have resulted in a growing demand for accountant specialists as educated professionals. The aim of the present research is to find out what is the role of the accountant in the modern enterprise. The authors conducted a survey among Estonian accounting specialists and managers. The results of the survey clearly indica...

  14. Factors Affecting SSR in Holstein Dairy Cows

    Directory of Open Access Journals (Sweden)

    Alireza Heravi Mosavi

    2016-08-01

    Full Text Available Introduction Secondary sex ratio (SSR is the proportion of males to females at birth. It has been shown in many different mammalian species, many factors are associated with SSR. Changes in secondary sex ratio in dairy cows is considered economically important and the ability to change it could affect the revenues and profitability of a dairy farm. Thus, sperm or embryo sexing techniques in recent years has attracted more attention. Most breed of dairy cattle are more likely to have female calf is born to use them as replacement heifers and in order to maintain their productive herd number. On the contrary, when the goal is the production of meat, bull calves due to higher growth rates and production efficiency, are more convenient and more economically efficient. The aim of present study was to investigate some key factors affecting SSR in Iranian Holstein cows. According to Fisher, the sex ratio in the population under the control of natural selection is not always the same. There is overwhelming evidence to support the theory that shows Fisher Primary and secondary sex ratio sex ratio can deviate from this balance and natural selection caused a change in this ratio can be in certain circumstances. For example, the secondary sex ratio of 52:48 has been reported in dairy cows. Studies on mammalian species suggest that several factors, including latitude of the location, the dominant regional climate model, time and frequency of mating to ovulation, diet, age of parents, physical score, breed and produced eggs from ovarian left or right can have a significant effect on the secondary sex ratio. Weather conditions may modify the internal environment and the effect on physiological mechanisms or through the impact on the frequency and type of foods available to parents, the secondary sex ratio is impressive. The impact on the quantity and quality of parent's access to food sources in many species of mammals, the sex ratio has been fixed. Previous

  15. Efficiency of Estonian grain farms in 2000 2004

    Directory of Open Access Journals (Sweden)

    N. VASILIEV

    2008-12-01

    Full Text Available The aim of this study is to analyse the efficiency of Estonian grain farms after Estonia’s transition to a market economy and during the accession period to the European Union (EU. The non-parametric method Data Envelopment Analysis (DEA was used to estimate the total technical, pure technical and scale efficiency of Estonian grain farms in 2000–2004. Mean total technical efficiency varied from 0.70 to 0.78. Of the grain farms 62% are operating under increasing returns to scale. Solely based on the DEA model it is not possible to determine optimum farm scale and the range of Estonian farm sizes operating efficiently is extensive. The most pure technically efficient farms were the smallest and the largest but the productivity of small farms is low compared to larger farms because of their small scale. Therefore, they are the least competitive. Since pre-accession period to the EU, large input slacks of capital have replaced the former excessive use of labour and land. This raises the question about the effects on efficiency of the EU’s investment support schemes in new member states.;

  16. Tallinn ostab Harju tänava krundid 150 miljoni krooni eest / Urmas Seaver

    Index Scriptorium Estoniae

    Seaver, Urmas, 1973-

    2006-01-01

    Ilmunud ka: Postimees : na russkom jazõke 26. jaan. lk. 4. Tallinn ostab OÜ-lt Celsea 150 miljoni krooni eest kuus krunti Harju tänava ääres, et rajada varemete asemele sügiseks haljasala. Tallinna abilinnapea Taavi Aasa sõnul ei ole tehing linnale kahjulik, linnavolikogu Isamaaliidu fraktsioon plaani ei toeta

  17. [Lea Kõiv. Paul Johansen und das Stadtarchiv Reval/Tallinn] / Paul Kaegbein

    Index Scriptorium Estoniae

    Kaegbein, Paul

    2008-01-01

    Arvustus: Lea Kõiv. Paul Johansen und das Stadtarchiv Reval/Tallinn. - Nordosteuropa als Geschichtsregion. Helsinki : Aue-Stiftung; Lübeck : Schmict-Röhmild, 2006. Lk. 45-59. Artiklis on kasutatud seni avalikkusele vähetuntud materjale ja osaliselt käsitletud avalikke vaidlusi, kuhu olid segatud nii Paul Johansen kui järgmine linnaarhivaar Rudolf Kenkmaa

  18. Public transport pricing policy : Empirical evidence from a fare-free scheme in Tallinn, Estonia

    NARCIS (Netherlands)

    Cats, O.; Reimal, T.; Susilo, Y.

    2014-01-01

    Cities worldwide are looking for new policies to attract travelers to shift from cars to public transport. Policies focused on reducing public transport fares are aimed at improving social inclusion and leading to a modal shift. The City of Tallinn, the capital of Estonia, has recently introduced a

  19. Madalmaade nukunäitlejate paremik hiilgas Tallinn Treffil / Tiiu Laks

    Index Scriptorium Estoniae

    Laks, Tiiu, 1984-

    2010-01-01

    5.-8. juunini 2010 toimuvast rahvusvahelisest festivalist Tallinn Treff. Hollandi trupi DudaPaiva lavastusest "Needus". Nukunäitleja Neville Tranteri (Austraalia-Holland) lavastusest "Punch ja Judy Afganistanis". Stef Vettersi (Belgia) etendusest "Tema kõrgeausus Ottfriedi tundmatu maailm"

  20. Euroopa kultuuripealinn Tallinn 2011 paneb PÖFF-ile õla alla

    Index Scriptorium Estoniae

    2011-01-01

    15. Pimedate Ööde Filmifestivali ja Euroopa Kultuuripealinna Tallinn 2011 koostöös alguse saanud noortele animavallas tegutsevatele professionaalidele mõeldud koolitussarjast AnimaCampus, rahvusvahelise filmituru Black Market ja programmi "60 sekundit üksindust aastal Null" toetamisest

  1. Bob Scott: Tallinn 2011 saab oma preemiaraha kätte / Bob Scott ; intervjueerinud Teele Tammeorg

    Index Scriptorium Estoniae

    Scott, Bob

    2010-01-01

    Euroopa kultuuripealinnade hindamiskomisjoni esimees räägib oma kohtumisest Tallinna linnapea Edgar Savisaare ja kultuuriministri Laine Jänesega ning sellest, et Tallinnal ja Eestil tuleb lisatoetuse saamiseks saata Euroopa Komisjonile vaid kiri, milles nad kinnitavad, et kultuuripealinna programmi jaoks on raha olemas

  2. Sex work in Tallinn, Estonia: the sociospatial penetration of sex work into society.

    Science.gov (United States)

    Aral, S O; St Lawrence, J S; Uusküla, A

    2006-10-01

    It is important to describe and understand the underlying patterns and dynamics that govern sex work in societies undergoing rapid political and social changes, its heterogeneity across populations, and its evolution through time in order to inform future research, sound policy formation, and programme delivery. To describe the socioeconomic and cultural determinants, organisational structure, distinct categories, and spatial patterning of sex work in Tallinn, Estonia, and identify recent temporal changes in sex work patterns. In-depth interviews with key informants; naturalistic observations of sex work and drug use venues, geo-mapping of sex work sites, review of media, public policy, and commissioned reports, and analyses of existing data. Sex work takes place in a hierarchy of locations in Tallinn ranging from elite brothels and "love flats" to truck stops. These sites vary in terms of their public health importance and social organisation. There are full time, part time, and intermittent male and female sex workers. Among others, the taxi driver, madam and the bartender are central roles in the organisation of sex work in Tallinn. Cell phone and internet technology enable sex work to be highly dispersed and spatially mobile. Future research and programmatic service delivery or outreach efforts should respond to the changing profile of sex work in Tallinn and its implications for STD/HIV epidemiology.

  3. Tallinn Fashion Week täidab nädala kodumaise moega / Karilin Engelbrecht

    Index Scriptorium Estoniae

    Engelbrecht, Karilin

    2009-01-01

    Tallinn Fashion Week'i üritustest. Ülevaade eesti moekunstnike loomingu esitlustest. Näitus "Uus Eesti moejoonistus" Arhitektuuri- ja Disainigaleriis. Anu Hindi moefotonäitus "Aeg maha 3" Iida galeriis. Raamatu "Eesti moepiibel 2010" esitlus Rotermanni kvartali kohvikus Platz. Oksana Tanditi ja Filippo Caroti moefotonäitus "Butterflies" Tallinna botaanikaaias

  4. Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers.

    Science.gov (United States)

    Missio, R F; Caixeta, E T; Zambolim, E M; Pena, G F; Zambolim, L; Dias, L A S; Sakiyama, N S

    2011-10-06

    Coffee is one of the main agrifood commodities traded worldwide. In 2009, coffee accounted for 6.1% of the value of Brazilian agricultural production, generating a revenue of US$6 billion. Despite the importance of coffee production in Brazil, it is supported by a narrow genetic base, with few accessions. Molecular differentiation and diversity of a coffee breeding program were assessed with gSSR and EST-SSR markers. The study comprised 24 coffee accessions according to their genetic origin: arabica accessions (six traditional genotypes of C. arabica), resistant arabica (six leaf rust-resistant C. arabica genotypes with introgression of Híbrido de Timor), robusta (five C. canephora genotypes), Híbrido de Timor (three C. arabica x C. canephora), triploids (three C. arabica x C. racemosa), and racemosa (one C. racemosa). Allele and polymorphism analysis, AMOVA, the Student t-test, Jaccard's dissimilarity coefficient, cluster analysis, correlation of genetic distances, and discriminant analysis, were performed. EST-SSR markers gave 25 exclusive alleles per genetic group, while gSSR showed 47, which will be useful for differentiating accessions and for fingerprinting varieties. The gSSR markers detected a higher percentage of polymorphism among (35% higher on average) and within (42.9% higher on average) the genetic groups, compared to EST-SSR markers. The highest percentage of polymorphism within the genetic groups was found with gSSR markers for robusta (89.2%) and for resistant arabica (39.5%). It was possible to differentiate all genotypes including the arabica-related accessions. Nevertheless, combined use of gSSR and EST-SSR markers is recommended for coffee molecular characterization, because EST-SSRs can provide complementary information.

  5. Olev Schults : SAS vajab Estonian Airi rahvusliku lennufirmana / Olev Schults ; interv. Andres Reimer

    Index Scriptorium Estoniae

    Schults, Olev

    2008-01-01

    Estonian Airi nõukogu esimees vastab küsimustele, kas SAS arendas Läti airBalticut Estonian SAS-i arvel, mis mõte on rahvuslikul lennukompaniil, kui riik ei tohi seda finantseerida, kuidas mõjutab investorite meeleolu SAS-i Eestis tabanud poliitikute kriitika tulv

  6. Estonian Air jäi kolmest Fokkerist ilma / Toivo Tänavsuu

    Index Scriptorium Estoniae

    Tänavsuu, Toivo

    2006-01-01

    Estonian Airil ei õnnestunud saada kolme väiksemat reaktiivlennukit, lennuparki püütakse mitmekesistada järgmistel aastatel. Sel aastal loodetakse saada juurde ühe Boeing 737-300. Lisa: Boeing: Estonian Air on tasemel

  7. The Representation of the Cold War in Three Estonian History Textbooks

    Science.gov (United States)

    Korbits, Keit

    2015-01-01

    The article looks at the discursive strategies different Estonian history textbooks employ to represent the Cold War period, and the "commonsense" ideologies instilled through these representations. The textbooks analysed include two history books dating back to the Estonian Soviet Socialist Republic and, for contrast, one written during…

  8. Meedia roll venekeelse elanikkonna informeerituse tagamisel ning identiteedi kujunemisel / Triin Vihalemm

    Index Scriptorium Estoniae

    Vihalemm, Triin, 1968-

    2000-01-01

    Venekeelse elanikkonna meediatarbimine on valdavalt elektroonsete kanalite keskne. Tabelid. Ilmunud ka: Integration of Estonian Society : Monitoring 2000. - Tallinn ; Integratsija Estonskogo obshtshestva : monitoring 2000. - Tallinn

  9. Generation and application of SSR markers in avocado

    International Nuclear Information System (INIS)

    Sharon, D.; Lavi, U.; Cregan, P.B.; Hillel, J.

    1998-01-01

    Simple Sequence Repeat (SSR) DNA markers were generated and applied to avocado. An SSR marker is based on a pair of primers which are synthesized on the basis of DNA sequences flanking a micro satellite. These markers are PCR based, quite polymorphic and abundant in several species. These are the markers, of choice in the human genome. The number of SSR markers in the avocado genome was calculated to be about 45,000, with the A/T micro satellite being the most frequent (1 in 40 kb). SSR markers are quite expensive to generate due to the required multi-step procedure; Screening a genomic library, about 66% of the positive clones turned out after sequencing to be SSR containing clones. In only about 55% of these, was it possible to synthesize primers and, of this group, only about 50% of the markers were useful for typing a specific family. Typing of five avocado cultivars using 59 SSR markers results in one to eight alleles per locus, mean heterozygosity ranging between 0.51 and 0.66 and gene diversity ranging between 0.42 and 0.66. The SSR markers were used to estimate the genetic relationships between various Persea species. The number of alleles in these species ranged between five and twelve with heterozygosity levels between 0.11-0.78 and gene diversity between 0.69-0.89. A preliminary genetic map, based on these SSR markers together with some DNA fingerprints (DFP) and randomly amplified polymorphic DNA (RAPD) markers, was drawn. The map consists of 12 linkage group having two to five markers each. Linkage analysis with several quantitative trait loci (QTLs) was performed by genetic typing and phenotypic assessment of the progeny of a controlled cross. The results of the interval mapping suggest that the gene(s) coding for the existence of fibers in the flesh, are probably linked to linkage group 3. (author)

  10. FullSSR: Microsatellite Finder and Primer Designer

    Directory of Open Access Journals (Sweden)

    Sebastián Metz

    2016-01-01

    Full Text Available Microsatellites are genomic sequences comprised of tandem repeats of short nucleotide motifs widely used as molecular markers in population genetics. FullSSR is a new bioinformatic tool for microsatellite (SSR loci detection and primer design using genomic data from NGS assay. The software was tested with 2000 sequences of Oryza sativa shotgun sequencing project from the National Center of Biotechnology Information Trace Archive and with partial genome sequencing with ROCHE 454® from Caiman latirostris, Salvator merianae, Aegla platensis, and Zilchiopsis collastinensis. FullSSR performance was compared against other similar SSR search programs. The results of the use of this kind of approach depend on the parameters set by the user. In addition, results can be affected by the analyzed sequences because of differences among the genomes. FullSSR simplifies the detection of SSRs and primer design on a big data set. The command line interface of FullSSR was intended to be used as part of genomic analysis tools pipeline; however, it can be used as a stand-alone program because the results are easily interpreted for a nonexpert user.

  11. New device based on the super spatial resolution (SSR) method

    International Nuclear Information System (INIS)

    Soluri, A.; Atzeni, G.; Ucci, A.; Bellone, T.; Cusanno, F.; Rodilossi, G.; Massari, R.

    2013-01-01

    Recently it have been described that innovative methods, namely Super Spatial Resolution (SSR), can be used to improve the scintigraphic imaging. The aim of SSR techniques is the enhancement of the resolution of an imaging system, using information from several images. In this paper we describe a new experimental apparatus that could be used for molecular imaging and small animal imaging. In fact we present a new device, completely automated, that uses the SSR method and provides images with better spatial resolution in comparison to the original resolution. Preliminary small animal imaging studies confirm the feasibility of a very high resolution system in scintigraphic imaging and the possibility to have gamma cameras using the SSR method, to perform the applications on functional imaging. -- Highlights: • Super spatial resolution brings a high resolution image from scintigraphic images. • Resolution improvement depends on the signal to noise ratio of the original images. • The SSR shows significant improvement on spatial resolution in scintigraphic images. • The SSR method is potentially utilizable for all scintigraphic devices

  12. "Tallinn Treff" - maailma lavade uus põlvkond Tallinnas / Meelis Pai, Vahur Keller, Reeda Toots ; intervjueerinud Margot Visnap

    Index Scriptorium Estoniae

    Pai, Meelis, 1968-

    2009-01-01

    30. maist 6. juunini toimuvast rahvusvahelisest nukuteatrifestivalist "Tallinn Treff" ning selle alafestivalist "Noor vaim" annavad ülevaate festivali peakorraldaja Meelis Pai, kusntiline juht Vahut Keller ja "Noor vaimu" juht Reeda Toots

  13. Programming Basics for Beginners. Experience of the Institute of Informatics at Tallinn University of Technology

    Directory of Open Access Journals (Sweden)

    Olga Mironova

    2017-11-01

    Full Text Available The present paper demonstrates the teaching approach in programming basics course for novices: schoolchildren of different ages and schoolteachers. This programming course was developed at the Institute of Informatics at Tallinn University of Technology in Tallinn, Estonia and it based on many years experience in teaching programming for non-IT first year students. The main aim of the chosen teaching approach in the course is to raise the motivation and keep the learners’ interest in programming field on the high level. The idea of developed teaching technique is the implementation of the visual programming before a serious textual coding. Furthermore, authors suggest readers some ways and methods to overcome learners’ difficulties in the first stage in a textual coding.

  14. SSR Analysis of Genetic Diversity Among 192 Diploid Potato Cultivars

    Directory of Open Access Journals (Sweden)

    Xiaoyan Song

    2016-05-01

    Full Text Available In potato breeding, it is difficult to improve the traits of interest at the tetraploid level due to the tetrasomic inheritance. A promising alternative is diploid breeding. Thus it is necessary to assess the genetic diversity of diploid potato germplasm for efficient exploration and deployment of desirable traits. In this study, we used SSR markers to evaluate the genetic diversity of diploid potato cultivars. To screen polymorphic SSR markers, 55 pairs of SSR primers were employed to amplify 39 cultivars with relatively distant genetic relationships. Among them, 12 SSR markers with high polymorphism located at 12 chromosomes were chosen to evaluate the genetic diversity of 192 diploid potato cultivars. The primers produced 6 to 18 bands with an average of 8.2 bands per primer. In total, 98 bands were amplified from 192 cultivars, and 97 of them were polymorphic. Cluster analysis using UPGMA showed the genetic relationships of all accessions tested: 186 of the 192 accessions could be distinguished by only 12 pairs of SSR primers, and the 192 diploid cultivars were divided into 11 groups, and 83.3% constituted the first group. Clustering results showed relatively low genetic diversity among 192 diploid cultivars, with closer relationship at the molecular level. The results can provide molecular basis for diploid potato breeding.

  15. A rapid situation assessment of the market for surrogate and illegal alcohols in Tallinn, Estonia.

    Science.gov (United States)

    Pärna, Kersti; Lang, Katrin; Raju, Kadi; Väli, Marika; McKee, Martin

    2007-01-01

    To understand the phenomenon of consumption of surrogate and illegal alcohols in Tallinn, capital of Estonia. This study, conducted in Tallinn in May 2006, used rapid situation assessment. Interviews with key informants in relevant settings such as emergency departments of hospitals, accommodation for the homeless, police etc. (n = 22), with alcohol abusers (n = 33), natural observations of surrogate sale and consumption venues (n = 46), and tracking of trade data were carried out. Key informants confirmed that consumption of illegal and surrogate alcohols are widely used by alcohol abusers, a finding confirmed by the alcohol abusers. Availability of surrogates varied by area of the city, mainly sold from street kiosks. Illegally produced spirits were also easily available. Sales of surrogates appear to have increased in recent years. A range of alcohol-containing substances that appear to be easily available at low cost, and that have high concentration of ethanol or contaminants known to be toxic, were identified in Tallinn. Alcohol policies in Estonia should address the consumption and availability of these substances.

  16. Estonian science breeds world-class researches / ToivoTänavsuu, Hille Tänavsuu

    Index Scriptorium Estoniae

    Tänavsuu, Toivo

    2010-01-01

    Eesti teadusfirmadest: biotehnoloogia ettevõtted Celecure AS, Protobios OÜ, Icosagen AS, Asper Biotech AS ja Solis BioDyne OÜ, geenitehnoloogia firma Genorama OÜ, keemiatehnoloogia ettevõte AS Cambrex Tallinn

  17. Acquisition of noun derivation in Estonian and Russian L1

    Directory of Open Access Journals (Sweden)

    Reili Argus

    2018-04-01

    Full Text Available Acquisition of derivation is not a well-studied area in first language research and a comparative approach to the acquisition of derivation in different languages doesn’t exist. There is no information on how a child acquires derivation in a language with a rich and regular system of derivational patterns, or in a language where derivation is productive, but the system of derivational patterns is opaque. According to general ideas of complexity in a language, the child should start to use simplex stems first and, only after that, complex ones, that is, complexity should increase in the course of acquisition. Our paper is intended to address these issues, based on longitudinal child data from typologically different languages, Estonian and Russian. The results revealed significant differences in the acquisition of noun derivation in the two languages under observation. The system of noun derivation is acquired at a faster pace in Russian, while Estonian children have far fewer noun derivatives in their speech and they use different derivation suffixes with less regularity. Even so, the so-called building block model may be applied for both languages only partially.

  18. First genetic linkage map of Taraxacum koksaghyz Rodin based on AFLP, SSR, COS and EST-SSR markers.

    Science.gov (United States)

    Arias, Marina; Hernandez, Monica; Remondegui, Naroa; Huvenaars, Koen; van Dijk, Peter; Ritter, Enrique

    2016-08-04

    Taraxacum koksaghyz Rodin (TKS) has been studied in many occasions as a possible alternative source for natural rubber production of good quality and for inulin production. Some tire companies are already testing TKS tire prototypes. There are also many investigations on the production of bio-fuels from inulin and inulin applications for health improvement and in the food industry. A limited amount of genomic resources exist for TKS and particularly no genetic linkage map is available in this species. We have constructed the first TKS genetic linkage map based on AFLP, COS, SSR and EST-SSR markers. The integrated linkage map with eight linkage groups (LG), representing the eight chromosomes of Russian dandelion, has 185 individual AFLP markers from parent 1, 188 individual AFLP markers from parent 2, 75 common AFLP markers and 6 COS, 1 SSR and 63 EST-SSR loci. Blasting the EST-SSR sequences against known sequences from lettuce allowed a partial alignment of our TKS map with a lettuce map. Blast searches against plant gene databases revealed some homologies with useful genes for downstream applications in the future.

  19. First genetic linkage map of Taraxacum koksaghyz Rodin based on AFLP, SSR, COS and EST-SSR markers

    Science.gov (United States)

    Arias, Marina; Hernandez, Monica; Remondegui, Naroa; Huvenaars, Koen; van Dijk, Peter; Ritter, Enrique

    2016-01-01

    Taraxacum koksaghyz Rodin (TKS) has been studied in many occasions as a possible alternative source for natural rubber production of good quality and for inulin production. Some tire companies are already testing TKS tire prototypes. There are also many investigations on the production of bio-fuels from inulin and inulin applications for health improvement and in the food industry. A limited amount of genomic resources exist for TKS and particularly no genetic linkage map is available in this species. We have constructed the first TKS genetic linkage map based on AFLP, COS, SSR and EST-SSR markers. The integrated linkage map with eight linkage groups (LG), representing the eight chromosomes of Russian dandelion, has 185 individual AFLP markers from parent 1, 188 individual AFLP markers from parent 2, 75 common AFLP markers and 6 COS, 1 SSR and 63 EST-SSR loci. Blasting the EST-SSR sequences against known sequences from lettuce allowed a partial alignment of our TKS map with a lettuce map. Blast searches against plant gene databases revealed some homologies with useful genes for downstream applications in the future. PMID:27488242

  20. Estonian horticultural peat marketing: sales promotion and price formation. 2. part

    International Nuclear Information System (INIS)

    Hammer, Hele

    1999-01-01

    When forming prices, Estonian peat companies' decisions should be based on marginal cost analysis. Unfortunately most Estonian companies sell peat to intermediaries and cannot influence its price. Estonian peat producers have to choose between either selling peat directly or selling through a central marketing organization. Both systems have their pros and cons. Direct selling gives more freedom to individual producers but is more risky. Central marketing makes cost saving possible and is more effective and stable, but may alienate producers from clients and markets. Whichever marketing system Estonian peat companies choose, the most important elements in their marketing strategy should be: careful market analysis, personal sales, attending trade shows, catalogues, quality service and offering transportation services. (author)

  1. Problems of contemporary ecology. Temporal changes in Estonian nature and environment

    International Nuclear Information System (INIS)

    Frey, T.

    1997-01-01

    This conference was held 8-9 May 1997 at Tartu, Estonia. The proceedings of the 7. Estonian Conference in Ecology contain the results of mostly original research in environmental science, conservation and natural philosophy

  2. SAS paneb Estonian Airi kiirmüüki / Gert D. Hankewitz

    Index Scriptorium Estoniae

    Hankewitz, Gert D.

    2009-01-01

    Majandusraskustes lennufirma SAS kavatseb loobuda osalustest kõigis lennufirmades, mis ei sobi kokku nende uue tegevuskavaga. Majandus- ja kommunikatsiooniminister Juhan Parts arvab, et riik peaks leidma Estonian Airile kodumaised partnerid

  3. Development and validation of new SSR markers from expressed regions in the garlic genome

    Directory of Open Access Journals (Sweden)

    Meryem Ipek

    2015-02-01

    Full Text Available Only a limited number of simple sequence repeat (SSR markers is available for the genome of garlic (Allium sativum L. despite the fact that SSR markers have become one of the most preferred DNA marker systems. To develop new SSR markers for the garlic genome, garlic expressed sequence tags (ESTs at the publicly available GarlicEST database were screened for SSR motifs and a total of 132 SSR motifs were identified. Primer pairs were designed for 50 SSR motifs and 24 of these primer pairs were selected as SSR markers based on their consistent amplification patterns and polymorphisms. In addition, two SSR markers were developed from the sequences of garlic cDNA-AFLP fragments. The use of 26 EST-SSR markers for the assessment of genetic relationship was tested using 31 garlic genotypes. Twenty six EST-SSR markers amplified 130 polymorphic DNA fragments and the number of polymorphic alleles per SSR marker ranged from 2 to 13 with an average of 5 alleles. Observed heterozygosity and polymorphism information content (PIC of the SSR markers were between 0.23 and 0.88, and 0.20 and 0.87, respectively. Twenty one out of the 31 garlic genotypes were analyzed in a previous study using AFLP markers and the garlic genotypes clustered together with AFLP markers were also grouped together with EST-SSR markers demonstrating high concordance between AFLP and EST-SSR marker systems and possible immediate application of EST-SSR markers for fingerprinting of garlic clones. EST-SSR markers could be used in genetic studies such as genetic mapping, association mapping, genetic diversity and comparison of the genomes of Allium species.

  4. SSR-CE/FD assessment of Guizhou approved sugarcane cultivars and regional materials

    Science.gov (United States)

    Twelve sugarcane genotypes (three cultivars and nine clones involved in regional tests) from Guizhou Province, China were analyzed using SSR-capillary electrophoresis/fluorescence detection (SSR-CE/FD) technology to construct the SSR fingerprints and assess the genetic diversity. A total of 131 DNA ...

  5. Cross-species amplification of 105 Lolium perenne SSR loci in 23 species within the Poaceae

    DEFF Research Database (Denmark)

    Jensen, Louise Bach; Holm, Preben Bach; Lübberstedt, Thomas

    2007-01-01

    Amplification of 105 Lolium perenne SSR markers was studied in 23 grass species representing seven tribes from three subfamilies of Poaceae. Twelve of the SSR markers are published for the first time. Between 2% and 96% of the SSR markers could be amplified within a given species. A subset of eight...

  6. Development of SSR markers and construction of a linkage map in jute

    Indian Academy of Sciences (India)

    In this communication, we report an additional set of 607 novel SSR in 393 SSR containing sequences. However, primers could be designed for only 417 potentially useful SSR. Polymorphism survey was carried out for 374 primer pairs using two parental genotypes (JRO 524 and PPO4) of a mapping population developed ...

  7. Identification of SSR and RAPD markers associated with QTLs of ...

    African Journals Online (AJOL)

    SERVER

    2008-04-03

    Apr 3, 2008 ... Parents and. F3 families had significant differences in studied traits (p ≤. 0.01). In this study, SSR and RAPD markers were used together for constructing linkage groups and rescanning the genome of rapeseed to identify QTLs controlling winter survival and related traits. For this, the parental polymorphism ...

  8. Analysis of B-genome derived simple sequence repeat (SSR ...

    African Journals Online (AJOL)

    A study was conducted to investigate the genetic variability between 40 Musa genotypes maintained at the Musa germplasm collection of the International Institute for Tropical Agriculture, Ibadan using nine B-genome derived simple sequence repeat (SSR) markers. The nine primers produced reproducible and discrete ...

  9. Segregation analysis of microsatellite (SSR) markers in sugarcane polyploids

    Science.gov (United States)

    Although the microsatellite (SSR) DNA markers have been extensively used in sugarcane breeding research, little is known about its inheritance mechanism. To address this problem, a high throughput molecular genotyping experiment was conducted on 964 single pollen grains and a 288-self progeny S1 map...

  10. Inheritance of blast resistance and identification of SSR marker ...

    Indian Academy of Sciences (India)

    2013-08-02

    Aug 2, 2013 ... visualized on Gel Documentation System (Flour ChemTM. Alpha Innotech Corporation, San Leandro, USA). The SSR markers found polymorphic .... ysis of segregating generations, breeding lines and varieties having RDN 98-2 as a parent. Acknowledgements. Authors thank the Head of the Department of ...

  11. Simple sequence repeat (SSR)-based genetic variability among ...

    African Journals Online (AJOL)

    The objective of this study was to compare if simple sequence repeat (SSR) markers could correctly identify peanut genotypes with difference in specific leaf weight (SLW) and relative water content (RWC). Four peanut genotypes and two water regimes (FC and 1/3 available water; 1/3 AW) were arranged in factorial ...

  12. Identification of SSR and RAPD markers associated with QTLs of ...

    African Journals Online (AJOL)

    Because of importance of winter survival in winter type of Brassica napus, this study was performed to identify the QTLs controlling winter survival and related traits using SSR and RAPD markers. For this, an F2:3 population of 200 families derived from crossing between cv. 'SLMO46' (winter type and cold resistant) and cv.

  13. Development of simple sequence repeat (SSR) markers that are ...

    African Journals Online (AJOL)

    ajl user 1

    2012-02-07

    Feb 7, 2012 ... 2Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657,. Japan. 3College ... on the genome. SSR is becoming increasingly recognized as useful DNA markers for genetic analysis, marker assisted selection, and other purposes (Gupta and Varshney ...

  14. SSR marker based DNA fingerprinting and diversity study in rice ...

    African Journals Online (AJOL)

    The genetic diversity and DNA fingerprinting of 15 elite rice genotypes using 30 SSR primers on chromosome numbers 7-12 was investigated. The results revealed that all the primers showed distinct polymorphism among the cultivars studied indicating the robust nature of microsatellites in revealing polymorphism. Cluster ...

  15. Comparative evaluation of genetic diversity using RAPD, SSR and ...

    Indian Academy of Sciences (India)

    High bootstrap values were obtained at major nodes of phenograms through WINBOOT software. Comparison of RAPD, SSR and cytochrome P450 gene based markers, in terms of the quality of data output, indicated that SSRs and cyt P450 gene based markers are particularly promising for the analysis of plant genome ...

  16. Evaluation of genetic diversity in rice using SSR markers

    African Journals Online (AJOL)

    hemant

    2012-10-18

    Oct 18, 2012 ... The polymorphism information content (PIC) value for the SSR loci ranged from 0.36 to. 0.98. Higher PIC values were associated ... Since each marker system has specific advantages and disadvantages, the choice of the marker ... The objective of current studies was to estimate the genetic diversity among ...

  17. Study of simple sequence repeat (SSR) polymorphism for biotic ...

    African Journals Online (AJOL)

    home

    2013-10-02

    Oct 2, 2013 ... parents studied indicating the robust nature of microsatellites in revealing polymorphism. Based on this study, the large range of similarity values for related cultivars using microsatellites provides greater confidence for the assessment of simple sequence repeats (SSR) polymorphism. Key words: Simple ...

  18. Genetic relationships revealed by simple sequence repeat (SSR ...

    African Journals Online (AJOL)

    Genetic relationships revealed by simple sequence repeat (SSR) markers among Ghanaian cassava cultivars released by different research groups. ... Genetic diversity was observed within populations (HS = 0.552) and, therefore, suggesting a low rate of inter-population gene flow among the individuals constituting the ...

  19. Genetic diversity studies and identification of SSR markers ...

    Indian Academy of Sciences (India)

    Supplementary data, J. Genet. 92, 273–280. Ta b le. 1 . Jaccard's similarity coefficients b etween. 36 pigeonpea g enotypes b ased on. 24 polymorphic. SSR markers. IPA-. KPL-. BDN-. BDN-. IPA-. BDN-. IPA-. ICP-. BWR-. BSMR-. IPA. -. IPA. -. B. DN-. BWR-. MAL-. NDA-. ICP-. Genotype. Bahar. 204. 43. 2010. 2029. 8F.

  20. Chromosomal location of genomic SSR markers associated with ...

    Indian Academy of Sciences (India)

    Chromosomal location of genomic SSR markers associated with yellow rust resistance in Turkish bread wheat (Triticum aestivum L.) ... Faculty of Science, Department of Molecular Biology and Genetics, Gebze Institute of Technology, Cayirova Campus, 41700, Gebze, Kocaeli, Turkey; The Scientific and Technological ...

  1. Using microsatellite (SSR) and morphological markers to assess the ...

    African Journals Online (AJOL)

    Falcata (Medicago sativa spp. falcata L.), with its high resistance to cold weather, drought and disease, plays an important role in alfalfa breeding. The aim of this study was to assess the genetic diversity of the 12 falcata populations in Eurasia using SSR markers and morphological traits. Regressions for genetic distance, ...

  2. Comparative evaluation of genetic diversity using RAPD, SSR and ...

    Indian Academy of Sciences (India)

    similarity index value of 0.505, 0.504 and 0.499, respectively. ... dendrograms and principal coordinate analysis (PCA) plots derived from the binary data matrices of the three marker systems ... Comparison of RAPD, SSR and cytochrome P450 gene based markers, in terms of the quality of data output, indicated that.

  3. Supplementary data: Development of SSR markers and construction ...

    Indian Academy of Sciences (India)

    Debabrata Sarkar, Mohit K. Sinha, Harindra S. Balyan and Pushpendra K. Gupta. J. Genet. 91, 21-31. Table 1. Details of 66 polymorphic SSR, primary motif, complexity, type, primer sequences and expected product size. GenBank Marker acc. no. name. Primary motif. Complexity Type. Forward primer (5'-3'). Reverse primer ...

  4. Trace metal emissions from the Estonian oil shale fired power

    DEFF Research Database (Denmark)

    Aunela-Tapola, Leena A.; Frandsen, Flemming; Häsänen, Erkki K.

    1998-01-01

    a two-fraction particle sampling and subsequent absorption of the gaseous fraction. The analyses were principally performed with ICP-MS techniques. The trace metal contents of Estonian oil shale were found to be in the same order of magnitude as of coal on average. The high total particle concentrations...... in the flue gases of the studied oil shale plant contribute, however, to clearly higher total trace metal emission levels compared to modern coal fired power plants. Although the old electrostatic precipitators in the plant have been partly replaced by state-of-the-art electrostatic precipitators......, significant portions of some heavy metals (e.g., Hg, Cd, As and Pb) were found in the absorption liquids of the sampling line, indicating the presence of either vaporous metal species or metals condensed on very small particles in the flue gases. The experimental results were interpreted by theoretical...

  5. Early vocabulary in full term and preterm Estonian children.

    Science.gov (United States)

    Schults, Astra; Tulviste, Tiia; Haan, Elis

    2013-09-01

    Preterm birth is considered to be a high risk factor for child development and early vocabulary can be used as an indicator for later development. The aim of the present study is to compare the size of early vocabulary, proportional use of different word categories, and mean length of utterance (henceforth MLU) of preterm and full term children. The sample consisted of 40 preterm (corrected ages 16-25 months) and two matched groups of full term children. First full term group consisted of 120 children who were matched by age and gender. Second full term group consisted of 109 children who were matched by age, gender and size of productive vocabulary. The data for this study were gathered using the Estonian adaptation of MacArthur-Bates Communicative Development Inventory: Words and Sentences. Full term children who were matched by age and gender had larger vocabulary as compared to the preterm children's vocabulary (U = 1758.5, p = 0.01). Poisson regression yielded that age, gender, and preterm birth explained significantly the variance in the vocabulary size. Poisson regressions showed that all three variables explained significantly variance in proportional use of social terms and predicates. Age had significant effect for proportional use of common nouns. Age and preterm birth had a significant effect on the proportional use of function words. MLU was shorter in preterm than in full term children (U = 1125.0, p = 0.002). Estonian preterm children's vocabulary is slightly smaller than full term children's vocabulary. There is a difference in the proportions of word categories used, as preterm children use more social terms, and less predicates, and function words. Copyright © 2013 Elsevier Ltd. All rights reserved.

  6. The Effect of the 2008 War on Estonian Public Confidence in International Organisations: A Mixed Methods Study

    NARCIS (Netherlands)

    Moes, Jeroen; Melchior, Inge; Gugushvili, Alexi

    2014-01-01

    When the so-called 5 Day War between Russia and Georgia occurred in the summer of 2008, the Estonian public reaction was especially passionate. Two major explanations stem from the strong identification of Estonians with the situation of Georgia (post-Soviet state, small country, Russian

  7. Jäätmetega kimpus Estonian Cell annab muda põldudele kompostiks / Ulvar Käärt

    Index Scriptorium Estoniae

    Käärt, Ulvar, 1982-

    2007-01-01

    Kundas asuv Estonian Celli tehas jagab ümberkaudsetele talunikele jäätmetest lahtisaamiseks kompostimissegu. Seadused ei sätesta, kuidas Estonian Cellis tekkivaid jäätmeid töödelda. Kommenteerib Peeter Eek: Aasta läbi ei saa muda põllule viia

  8. Musical Practices and Methods in Music Lessons: A Comparative Study of Estonian and Finnish General Music Education

    Science.gov (United States)

    Sepp, Anu; Ruokonen, Inkeri; Ruismäki, Heikki

    2015-01-01

    This article reveals the results of a comparative study of Estonian and Finnish general music education. The aim was to find out what music teaching practices and approaches/methods were mostly used, what music education perspectives supported those practices. The data were collected using questionnaires and the results of 107 Estonian and 50…

  9. On the System of Place Name Signs in Estonian Sign Language

    Directory of Open Access Journals (Sweden)

    Liina Paales

    2011-05-01

    Full Text Available A place name sign is a linguistic-cultural marker that includes both memory and landscape. The author regards toponymic signs in Estonian Sign Language as representations of images held by the Estonian Deaf community: they reflect the geographical place, the period, the relationships of the Deaf community with hearing community, and the common and distinguishing features of the two cultures perceived by community's members. Name signs represent an element of signlore, which includes various types of creative linguistic play. There are stories hidden behind the place name signs that reveal the etymological origin of place name signs and reflect the community's memory. The purpose of this article is twofold. Firstly, it aims to introduce Estonian place name signs as Deaf signlore forms, analyse their structure and specify the main formation methods. Secondly, it interprets place-denoting signs in the light of understanding the foundations of Estonian Sign Language, Estonian Deaf education and education history, the traditions of local Deaf communities, and also of the cultural and local traditions of the dominant hearing communities. Both perspectives - linguistic and folkloristic - are represented in the current article.

  10. Standardized SSR allele naming and binning among projects.

    Science.gov (United States)

    Deemer, Dennis L; Nelson, C Dana

    2010-11-01

    Simple sequence repeats (SSRs) have proven to be extremely valuable DNA markers for genetic mapping and population genetic analyses. However, data collected across laboratories or even within laboratories are difficult to combine due to challenges in standardizing allele names, especially for nonmodel systems. Here we provide a new approach for standardizing SSR allele names that combines several previously recognized components for standardization, including reference samples/alleles, cumulative binsets, static between-allele spacing, and interval allele naming.

  11. Genetic parameters for milk coagulation properties in Estonian Holstein cows.

    Science.gov (United States)

    Vallas, M; Bovenhuis, H; Kaart, T; Pärna, K; Kiiman, H; Pärna, E

    2010-08-01

    The objective of this study was to estimate heritabilities and repeatabilities for milk coagulation traits [milk coagulation time (RCT) and curd firmness (E(30))] and genetic and phenotypic correlations between milk yield and composition traits (milk fat percentage and protein percentage, urea, somatic cell count, pH) in first-lactation Estonian Holstein dairy cattle. A total of 17,577 test-day records from 4,191 Estonian Holstein cows in 73 herds across the country were collected during routine milk recordings. Measurements of RCT and E(30) determined with the Optigraph (Ysebaert, Frepillon, France) are based on an optical signal in the near-infrared region. The cows had at least 3 measurements taken during the period from April 2005 to January 2009. Data were analyzed using a repeatability animal model. There was substantial variation in milk coagulation traits with a coefficient of variation of 27% for E(30) and 9% for the log-transformed RCT. The percentage of variation explained by herd was 3% for E(30) and 4% for RCT, suggesting that milk coagulation traits are not strongly affected by herd conditions (e.g., feeding). Heritability was 0.28 for RCT and 0.41 for E(30), and repeatability estimates were 0.45 and 0.50, respectively. Genetic correlation between both milk coagulation traits was negligible, suggesting that RCT and E(30) have genetically different foundations. Milk coagulation time had a moderately high positive genetic (0.69) and phenotypic (0.61) correlation with milk pH indicating that a high pH is related to a less favorable RCT. Curd firmness had a moderate positive genetic (0.48) and phenotypic (0.45) correlation with the protein percentage. Therefore, a high protein percentage is associated with favorable curd firmness. All reported genetic parameters were statistically significantly different from zero. Additional univariate random regression analysis for milk coagulation traits yielded slightly higher average heritabilities of 0.38 and 0

  12. [Heinz von zur Mühlen. Paul Johansen und die Sogenannten Undeutschen in Reval/Tallinn] / Paul Kaegbein

    Index Scriptorium Estoniae

    Kaegbein, Paul

    2008-01-01

    Arvustus: Heinz von zur Mühlen. Paul Johansen und die Sogenannten Undeutschen in Reval/Tallinn. - Nordosteuropa als Geschichtsregion. Helsinki : Aue-Stiftung :; Lübeck : Schmidt-Römhild, 2006. lk. 103-111. Paul Johanseni lähim töökaaslane kirjeldab teose "Deutsch und Undeutsch im mittelalterlichen und frühneuzeitlichen Reval" valmimist

  13. Uudised : Preemia "edition 49" noodigraafikale. Noor muusik ئ Tallinn 2000. Bergenisse sõidab Bezrodnaja / Hugo Alter

    Index Scriptorium Estoniae

    Alter, Hugo-Eduard, 1929-2003

    2000-01-01

    Kirjastuse "Edition 49" noodigraafikaga teos võitis muusikakirjastuspreemia "Frankfurt 2000". 6.-9. juunini toimub rahvusvaheline konkurss "Noor muusik ئ Tallinn 2000", millest võtavad osa kuni 13 aastased muusikud 11-st riigist. "Klassika-Eurovisiooni" Eesti võistluse finaali võitis A.-L. Bezrodnaja, kes esindab Eestit võistluse Euroopa finaalis juunis Bergenis

  14. Tallinn jäi kultuuripealinna projekti rahastamisel hätta / Maria-Elisa Rannajõe

    Index Scriptorium Estoniae

    Rannajõe, Maria-Elisa

    2007-01-01

    Järgmise aasta riigieelarvest on projekti "Tallinn Euroopa kultuuripealinn 2011" rahastamiseks eraldatud ette nähtud summast 10 miljonit krooni vähem. Tallinna abilinnapea Kaia Jäppineni sõnul on linn palunud Brüsselilt ajapikendust riigiga kokkuleppele jõudmiseks, pikendust anti 2-3 kuud

  15. Compiling the Dictionary of Word Associations in Estonian: From scratch to the database

    Directory of Open Access Journals (Sweden)

    Ene Vainik

    2018-04-01

    Full Text Available The present paper describes the project titled “The Dictionary of Word Associations in Estonian” undertaken by the author at the Institute of the Estonian Language. The general aim of the Dictionary is to provide insights into Estonians’ common-sense mind. It is meant to be a tool of self-reflection for Estonian native speakers and a guide for the foreigners who are eager enough to make themselves familiar with the Estonian cultural patterns of thought. The Dictionary will be published online. The number of keywords was initially limited to approximately 800. Specific emphasis is given to the stage of data collection by implementing the principles of citizen science.

  16. The Woman as Wolf (AT 409: Some Interpretations of a Very Estonian Folk Tale

    Directory of Open Access Journals (Sweden)

    Merili Metsvahi

    2013-12-01

    Full Text Available The article analyses tale type The Woman as Wolf, which is one of the most popular folk tales in the Estonian Folklore Archives and is represented there both in the form of a fairy tale and in the form of a legend. The vast majority of the versions of The Woman as Wolf were written down in the first part of the 20th century within Estonia and where recorded from Estonians. The article introduces the content of the tale, the origin of the first records from the early 19th century, and the dissemination area of the tale, which remains outside Western Europe: apart from the Estonian versions there are Sami, Karelian, Vepsian, Livonian, Latvian, Lithuanian, Russian, Ukrainian and Belarusian versions. While in almost all the Estonian versions the main protagonist is transformed into a wolf, in most of the versions written down in other areas and ethnic groups, another animal or bird replaces the wolf. The author is of the opinion that the Finnic area is central to the distribution of the folk tale The Woman as Wolf. The animal the woman is transformed into in the plot would not have been a wolf in earlier times. The article provides an explanation why the wolf is predominant in Estonian written sources. For that purpose the ways in which the wolf and werewolf were perceived in earlier Estonian folk belief are introduced. At the end of the article interpretation of the folk tale is provided. The author states that the plot and some of the motifs found in this folk tale reflect the difficulties women had in submitting to the norms and values of patriarchal order within their society.

  17. The Woman as Wolf (AT 409: Some Interpretations of a Very Estonian Folk Tale

    Directory of Open Access Journals (Sweden)

    Merili Metsvahi

    2014-01-01

    Full Text Available The article analyses tale type The Woman as Wolf, which is one of the most popular folk tales in the Estonian Folklore Archives and is represented there both in the form of a fairy tale and in the form of a legend. The vast majority of the versions of The Woman as Wolf were written down in the first part of the 20th century within Estonia and where recorded from Estonians. The article introduces the content of the tale, the origin of the first records from the early 19th century, and the dissemination area of the tale, which remains outside Western Europe: apart from the Estonian versions there are Sami, Karelian, Vepsian, Livonian, Latvian, Lithuanian, Russian, Ukrainian and Belarusian versions. While in almost all the Estonian versions the main protagonist is transformed into a wolf, in most of the versions written down in other areas and ethnic groups, another animal or bird replaces the wolf. The author is of the opinion that the Finnic area is central to the distribution of the folk tale The Woman as Wolf. The animal the woman is transformed into in the plot would not have been a wolf in earlier times. The article provides an explanation why the wolf is predominant in Estonian written sources. For that purpose the ways in which the wolf and werewolf were perceived in earlier Estonian folk belief are introduced. At the end of the article interpretation of the folk tale is provided. The author states that the plot and some of the motifs found in this folk tale reflect the difficulties women had in submitting to the norms and values of patriarchal order within their society.

  18. MODELLING DICLOFENAC AND IBUPROFEN RESIDUES IN MAJOR ESTONIAN SEASIDE CITIES

    Directory of Open Access Journals (Sweden)

    Erki Lember

    2016-06-01

    Full Text Available A theoretical model was developed to model the fate of two common pharmaceutical residues: diclofenac and ibuprofen in eight Estonian seaside cities that discharge their wastewaters directly into the Baltic Sea. The consumption rates of the active ingredients of diclofenac and ibuprofen from 2006-2014 were analysed. A decrease of 19.9% for diclofenac consumption and an increase of 14.1% for ibuprofen were found. The fate of diclofenac and ibuprofen were modelled by considering the human metabolism removal rate for pharmaceuticals, the removal rate of diclofenac and ibuprofen in activated sludge wastewater treatment plants (WWTP and annual flow rates. An average decrease from 1 to 0.8 µg/l (decrease of 20% for diclofenac and an increase from 11.4 to 13.4 µg/l (increase of 14.9% for ibuprofen for the concentration in the effluents of the WWTP were modelled. The model gives us a good overview about the theoretical concentrations of pharmaceutical residues in the environment and is helpful for evaluating environmental impacts.

  19. Chernobyl is still haunting us. Radionuclides in Estonian mushrooms

    International Nuclear Information System (INIS)

    Martin, J.; Vilde, R.; Martin, L.; Aaspollu, J.; Tekko, S.

    1993-01-01

    The disaster that happened at the Chernobyl nuclear power station in 1986 caused a sharp rise in radioactive pollution over an extensive area in the region of the Baltic Sea. To estimate the distribution and the concentration of Cs-137 and Sr-90 in Estonian ecosystems, samples of mushrooms, mosses, lichens and the upper horizons of soil were gathered from 63 test sites during 1991. We were particularly interested in the amounts of radionuclides in mushrooms because these are used as food by people. Dangerously high radionuclide concentrations were found in mushrooms gathered in North-East Estonia. Heightened concentrations were registered here and there all over the territory of Estonia, especially in mushrooms gathered in Central and South-Western Estonia. The Cs-137 content in mushrooms depends on its content in other components of the ecosystems, first and foremost on the concentrations of radiocaesium in mosses and litter, which, therefore, can be used as indicators in prognostication the radioactive pollution of mushrooms in a certain region. As Cs-137 migrates between various ecosystem components, it is necessary to check the radioactivity of the forest products used for food for still a number of years to come. The Sr-90 level was low in all the ecosystem components examined. (author). 3 figs., 10 refs

  20. The figure of the teacher in Estonian school discourse

    Directory of Open Access Journals (Sweden)

    Ott Puumeister

    2014-10-01

    Full Text Available The article concerns itself with the figure of the teacher in Estonian society. We do not concentrate on the educational system as a whole, but on one specific and crucial element in this apparatus - the teacher. We begin by offering a brief historical overview of the conditions of pedagogues in the 20th century before moving on to describe the adoption of neo-liberal free market policies since the 1990s and the effects these policies had and still have on education. Our main concern is to understand the teacher as an actor in power relations; to achieve this understanding we have selected as our examples 1 surveillance techniques in school environment that have direct relations to the state and the market; and 2 the 2012 educational workers' strike that made it quite clear that the teachers have been fixed to a position of wage workers. The overall and more abstract aim of the paper is to think about the social role of the teacher in Estonia.

  1. Environmental innovation through transport policy. The implementation of the free fare policy on public transport in Tallinn, Estonia

    Energy Technology Data Exchange (ETDEWEB)

    Gabaldon-Estevan, D.

    2016-07-01

    Urban areas are of increasing relevance when it comes to sustainability: First, about half of the world’s population now lives in cities (increasing to 60% by 2030). Second, cities are nowadays responsible for levels of resource consumption and waste generation that are higher beyond their share on world population. Third, cities are more vulnerable to disruptive events that can lead to restrictions on the provision of resources and to changes on the environment caused by climate change. And fourth, because they concentrate key resources (political, social, cultural…), cities are seen as strategic scenarios where to experiment and develop solutions to cope with the prevailing sustainability challenges driven by the major social and environmental transformations. Urban agglomerations can be seen as complex innovation systems where human activities are shaped in order to transform societies towards sustainable development. For this paper, we focus on the case of an environmental innovation regarding transport policy, the implementation of the fare-free policy on public transport for all inhabitants of Tallinn, Estonia. Tallinn, with 414,000 inhabitants in 2015, is the capital of Estonia and the largest city in the country. Over the last two decades the share of public transport trips decreased dramatically. After a public opinion poll in 2012, in which over 75% of the participants voted for a fare-free public transportation system (FFPTS) in Tallinn, the new policy was implemented on 1st January 2013. From that date on inhabitants of Tallinn could use all public transport services (busses, trams, trolly-busses) operated by city-run operators for free. Later the fare-free system was implemented also on trains within Tallinn. In this paper we analyze the context, in which this policy was implemented, the main characteristics of its implementation and its actual situation. (Author)

  2. Estonian Perceptions of Security: Not Only About Russia and the Refugees

    Directory of Open Access Journals (Sweden)

    Veebel Viljar

    2016-12-01

    Full Text Available The current study focuses on the Estonian perceptions of security and on the defence situation both globally and locally. The dynamic results of the public opinion surveys on security risks conducted in Estonia over the last 10 years (2006-2016 will be presented. In addition, to understand whether some of the security risks could be over- or underestimated in Estonia, these results will be compared with the views expressed recently by the World Economic Forum, particularly the Global Risks Report 2016. Also, the arguments why some topics have played or are currently playing key role in the Estonian security perception will be presented and discussed.

  3. Ancient buried valleys in the city of Tallinn and adjacent area

    Directory of Open Access Journals (Sweden)

    Vaher, Rein

    2010-03-01

    Full Text Available The distribution, morphology, fillings, and origin of buried valleys are discussed. The direction of the valleys varies from NW to NE. Within the Viru-Harju Plateau the valleys have a more or less symmetric profile, but asymmetric profiles are dominating in the pre-klint area. They are mainly filled with glacial (till, glaciofluvial (sand, gravel, and pebbles, glacio­lacustrine (varved clay, and marine (fine-grained sand deposits. The Tallinn valley with its tributary valleys (Saku and Sausti and fore-klint branches (Harku, Lilleküla, and Kadriorg looks like a river system. The fore-klint branches extend over 20 km in the Gulf of Finland. They are probably tributaries of the ancient river Pra-Neva. Most likely, the formation of valleys was continuous, starting from pre-Quaternary river erosion, and was sculptured by variable processes during the ice ages and influenced by flowing water during the interglacial periods.

  4. Eesti Tervishoiu Muuseum = Estonian Health Care Museum / Raili Paling, Liis Lindvere, Kadri Kaldam ; kommenteerinud Kadri Liimal

    Index Scriptorium Estoniae

    Paling, Raili

    2016-01-01

    Eesti Tervishoiu Muuseumi (Tallinn, Lai tn. 30) renoveeritud interjöör ja püsiekspositsioon. Autorid Jelena Altmäe, Kadri Kaldam, Andrus Kõresaar, Indrek Mikk, Jaanus Männik, Lea Laidra, Liis Lindvere, Raili Paling; kuraator Ülle Kask

  5. Postmodern diversity in Estonian architecture in the 70s and 80s / Marieke van Rooy

    Index Scriptorium Estoniae

    Rooy, Marieke van, 1974-

    2009-01-01

    Ülevaade Eesti Arhitektuurimuuseumis 28. 06.-24. 08. 2008 olnud näitusest ja kataloogist "Keskkond, projektid, kontseptsioonid. Tallinna Kooli arhitektid 1972-1985 = Environment, projects, concepts. Architects of the Tallinn School 1972-1985". Näituse kuraatorid ja kataloogi koostajad Andres Kurg ja Mari Laanemets

  6. Vabariigi Valitsuse istungitesaali mööbel = Furniture for the Estonian Government cabinet's meeting room

    Index Scriptorium Estoniae

    2008-01-01

    Stenbocki Maja istungitesaali (Rahukohtu 3, Tallinn) mööbel. Disainerid: Katrin Vaikla, Argo Vaikla (Vaikla Disain), Liis Tarbe (Torpedo Interior OÜ). Argo ja Katrin Vaiklast, Vaikla Disaini tähtsamad tööd. Liis Tarbest, koostöös Vaikla Disainiga valminud tööd. 6 värv. vaadet, fotod sisearhitektidest

  7. The problems and development potential of revenue autonomy in Estonian municipalities. Kohalike omavalitsuste tuluautonoomia probleemid ja arenguvõimalused Eestis

    Directory of Open Access Journals (Sweden)

    Janno Reiljan

    2013-01-01

    Full Text Available In a regionally heterogeneous country like Estonia, it is a difficult task to create a local government revenue structure that guarantees even supply of public services across the entire country and, at the same time, revenue autonomy for the municipalities. In the theoretical part of the current article the suitability of different sources of own revenues are analysed in the context of Estonian municipalities. The empirical part of the article compares the financing principles of Estonian municipalities with other EU countries. Finally, the proportions of different own sources of revenues in the budgets of Estonian local governments are examined and suggestions are made for changing the current system

  8. Estonian electricity supply scenarios for 2020 and their environmental performance

    Energy Technology Data Exchange (ETDEWEB)

    Koskela, Sirkka; Seppaelae, Jyri; Hiltunen, Marja-Riitta [Research Programme for Production and Consumption, Finnish Environment Institute, P.O. Box 140, 00251 Helsinki (Finland); Lipp, Ando; Pold, Evelin; Talve, Siret [CyclePlan Ltd, Akadeemia road 33, 12618 Tallinn (Estonia)

    2007-07-15

    Estonia is the only country in Europe with significant environmentally intensive oil shale-based energy production. However, the legal obligations of the EU will make substantial changes over the coming years to current electricity production technology. Increasing the use of alternative energy carriers for responding to future requirements has also been in focus. In this study, three different future electricity supply scenarios for Estonia in 2020 are considered and compared to the situation in 2002. They are based on domestic oil shale, imported natural gas, and imported nuclear power. According to the aims of the national energy policy, renewable energy sources were raised to 10% in all scenarios. Using the LCA methodology, the least damaging impact on the environment occurs in the 'nuclear scenario', with nuclear energy as the main energy source. The best scenario, however, depends on the weight or acceptance of accidental releases or other impacts not defined in this context. The 'Oil shale scenario' would be a slightly more damaging alternative than the 'Natural gas scenario' even if new technical solutions will remarkably improve the environmental performance of oil shale electricity production. Land use and waste disposal are crucial issues, particularly for oil shale and nuclear electricity production. However, the depletion of oil shale is not as critical an issue as the depletion of natural gas and uranium. According to the significance analysis of impact categories, climate change is the most significant impact on the environment in the scenarios. Future decisions on the development of the Estonian energy sector are most likely to be based on technological, economical and political aspects. Political aspects are likely to be the most significant. However, this type of study can give additional value to the discussion due to the increasing role of sustainability in energy issues. (author)

  9. An epidemiological perspective of personalized medicine: the Estonian experience.

    Science.gov (United States)

    Milani, L; Leitsalu, L; Metspalu, A

    2015-02-01

    The Estonian Biobank and several other biobanks established over a decade ago are now starting to yield valuable longitudinal follow-up data for large numbers of individuals. These samples have been used in hundreds of different genome-wide association studies, resulting in the identification of reliable disease-associated variants. The focus of genomic research has started to shift from identifying genetic and nongenetic risk factors associated with common complex diseases to understanding the underlying mechanisms of the diseases and suggesting novel targets for therapy. However, translation of findings from genomic research into medical practice is still lagging, mainly due to insufficient evidence of clinical validity and utility. In this review, we examine the different elements required for the implementation of personalized medicine based on genomic information. First, biobanks and genome centres are required and have been established for the high-throughput genomic screening of large numbers of samples. Secondly, the combination of susceptibility alleles into polygenic risk scores has improved risk prediction of cardiovascular disease, breast cancer and several other diseases. Finally, national health information systems are being developed internationally, to combine data from electronic medical records from different sources, and also to gradually incorporate genomic information. We focus on the experience in Estonia, one of several countries with national goals towards more personalized health care based on genomic information, where the unique combination of elements required to accomplish this goal are already in place. © 2014 The Authors. Journal of Internal Medicine published by John Wiley & Sons Ltd on behalf of The Association for the Publication of the Journal of Internal Medicine.

  10. The Estonian diaspora in South-West Russia in the 1920—30s: migration results

    Directory of Open Access Journals (Sweden)

    Stupin Yuri

    2010-11-01

    Full Text Available This article analyses the spatial features of the settling of Russian Estonians in the Northwest region at the “zenith” of diaspora on the basis of 1920, 1926, and 1939 censuses. The author identifies the principal settling areas and points out the geographical preconditions for the rapid decline of the diaspora.

  11. Gender Advantages and Gender Normality in the Views of Estonian Secondary School Students

    Science.gov (United States)

    Kuurme, Tiiu; Kasemaa, Gertrud

    2015-01-01

    The aim of the study on Estonian secondary school students was to obtain an overview of the gender-related views and experiences of the everyday school life by students, and to analyse the school-related factors in the development of gender roles and gender-related expectations. We view gender equality as a central condition for social…

  12. Alberta's Estonians 1899 - Present TLÜ Akadeemilises Raamatukogus / Sander Jürisson

    Index Scriptorium Estoniae

    Jürisson, Sander

    2014-01-01

    Tallinna Ülikooli Akadeemilises Raamatukogus on üleval näitus "Alberta's Estonians 1899 - Present", mis annab ülevaate Kanada Alberta provintsi eestlaste loost. Näitus valmis Alberta Eesti Kultuuripärandi Seltsi koostöös Alberta Provintsi Arhiivi Kultuuripärandi Osakonnaga Edmontonis

  13. The Perceived Impact of External Evaluation: The System, Organisation and Individual Levels-Estonian Case

    Science.gov (United States)

    Seema, Riin; Udam, Maiki; Mattisen, Heli; Lauri, Liia

    2017-01-01

    The purpose of this article is to provide an overview of how the employees of higher education institutions perceive the impact of external evaluations. The study was conducted using the concurrent mixed method and involved 361 employees from Estonian universities and professional higher education institutions. The results indicated that…

  14. Consumer Socialisation and Value Orientations among Estonian and Chinese Young People

    Science.gov (United States)

    Waerdahl, Randi; Kalmus, Veronika; Keller, Margit

    2011-01-01

    This paper asks if Estonian and Chinese tweens' access to pocket money influences their brand valuation, as well as value orientations in the context of perceived peer popularity and personal well-being. Surveys conducted in autumns 2006 (China n = 188) and 2007 (Estonia n = 111) show an inherent cultural resistance among tweens in both countries…

  15. Estonian Air võitis reisijaid juurde. Samas turuosa vähenes

    Index Scriptorium Estoniae

    2011-01-01

    Estonian Airi turuosa vähenes Tallinna lennujaamas regulaarliinidel 2010. aastal võrreldes 2009. aastaga 3,9% võrra, lende toimus 1,8% rohkem kui 2009. a. Lendude toimumise protsent oli 98,1, väljumise täpsus oli 86,7%. Andmeid ka 2010. aasta detsembrikuu kohta

  16. Tiger in Focus--A National Survey of ICT in Estonian Schools

    Science.gov (United States)

    Toots, Anu; Laanpere, Mart

    2004-01-01

    Estonia has not participated in international studies of ICT in education, nor have there been any similar studies at the national level up until the year 2000. The first survey of ICT in Estonian schools was conducted after completion of the national school computerization programme called Tiger Leap. This paper focuses on the targeted responses…

  17. Estonian Cell peab suu kasumist puhtaks pühkima / Kaisa Tahlfeld, Katre Pilvinski

    Index Scriptorium Estoniae

    Tahlfeld, Kaisa

    2008-01-01

    Estonian Cell saatis valitsusele pöördumise, milles palutakse teha parandusi elektrienergiaga seonduvates seadustes, mis aitaksid ettevõtet energia hinnatõusu ajal. Elektri hinnatõusu tõttu on muutunud küsitavaks ka Nitroferti tehase edasine eksisteerimine

  18. SAS võib omapoolse panusena kustutada osa Estonian Airi laenust / Gert D. Hankewitz

    Index Scriptorium Estoniae

    Hankewitz, Gert D.

    2011-01-01

    Lennufirma SAS investorsuhete asepresidendi Sture Stoleni sõnul ei ole tõenäoline, et nende firma maksaks mingit raha Estonian Airi kapitali, küll aga ei saa välistada võimalust, et võidakse kustutada osa antud laenust

  19. Introducing borrowings in the late 18th century : the Estonian translation of a Swedish cookbook by Cajsa Warg / Raimo Raag

    Index Scriptorium Estoniae

    Raag, Raimo, 1953-

    2002-01-01

    Rootsi kokaraamatu tõlkest eesti keelde ja selle osast eesti keele arengus: Warg, Christina. Köki ja Kokka Ramat, mis Rootsi kelest Eesti-ma Kele üllespandud on / [tlk. Johann Luthander]. Tallinn : [s.n.], 1781

  20. From nation-scape to nation-state : reconfiguring filmic space in post -soviet Estonian cinema / Eva Näripea

    Index Scriptorium Estoniae

    Näripea, Eva, 1980-

    2011-01-01

    Käsitleb nihkeid ruumikujutuses perestroikaaegses ja vahetult taasiseseisvumisjärgses Eesti filmikunstis, võrreldes kahte mängufilmi : Peeter Urbla "Ma pole turist, ma elan siin"(1988) ja Ilkka Järvilaturi "Tallinn pimeduses" (1993)

  1. Analysis of inheritance mode in chrysanthemum using EST-derived SSR markers

    NARCIS (Netherlands)

    Park, Sang Kun; Arens, Paul; Esselink, Danny; Lim, Jin Hee; Shin, Hak Ki

    2015-01-01

    To study the inheritance mode of hexaploid chrysanthemum (random or preferential chromosome pairing), a segregation analysis was carried out using SSR markers derived from chrysanthemum ESTs in the public domain. A total of 248 EST-SSR primer pairs were screened in chrysanthemum cultivars

  2. SAT, a flexible and optimized Web application for SSR marker development.

    Science.gov (United States)

    Dereeper, Alexis; Argout, Xavier; Billot, Claire; Rami, Jean-François; Ruiz, Manuel

    2007-11-29

    Simple Sequence Repeats (SSRs), or microsatellites, are among the most powerful genetic markers known. A common method for the development of SSR markers is the construction of genomic DNA libraries enriched for SSR sequences, followed by DNA sequencing. However, designing optimal SSR markers from bulk sequence data is a laborious and time-consuming process. SAT (SSR Analysis Tool) is a user-friendly Web application developed to minimize tedious manual operations and reduce errors. This tool facilitates the integration, analysis and display of sequence data from SSR-enriched libraries.SAT is designed to successively perform base calling and quality evaluation of chromatograms, eliminate cloning vector, adaptors and low quality sequences, detect chimera or partially digested sequences, search for SSR motifs, cluster and assemble the redundant sequences, and design SSR primer pairs. An additional virtual PCR step establishes primer specificity. Users may modify the different parameters of each step of the SAT analysis. Although certain steps are compulsory, such as SSR motifs search and sequence assembly, users do not have to run the entire pipeline, and they can choose selectively which steps to perform. A database allows users to store and query results, and to redo individual steps of the workflow. The SAT Web application is available at http://sat.cirad.fr/sat, and a standalone command-line version is also freely downloadable. Users must send an email to the SAT administrator tropgene@cirad.fr to request a login and password.

  3. Development and validation of new SSR markers from expressed regions in the garlic genome

    Science.gov (United States)

    Limited number of simple sequence repeat (SSR) markers is available for the genome of garlic (Allium sativum L.) although SSR markers have become one of the most preferred marker systems because they are typically co-dominant, reproducible, cross species transferable and highly polymorphic. In this ...

  4. Development of SSR markers and construction of a linkage map in jute

    Indian Academy of Sciences (India)

    2012-04-13

    Apr 13, 2012 ... [Das M., Banerjee S., Dhariwal R., Vyas S., Mir R. R., Topdar N., Kundu A., Khurana J. P., Tyagi A. K., Sarkar D., Sinha M. K., Balyan H. S. and Gupta P. K. 2012 Development of SSR markers .... under High Performance UV Transilluminator (Ultra Violet. Products, Cambridge, UK). Polymorphic SSR bands ...

  5. SAT, a flexible and optimized Web application for SSR marker development

    Directory of Open Access Journals (Sweden)

    Rami Jean-François

    2007-11-01

    Full Text Available Abstract Background Simple Sequence Repeats (SSRs, or microsatellites, are among the most powerful genetic markers known. A common method for the development of SSR markers is the construction of genomic DNA libraries enriched for SSR sequences, followed by DNA sequencing. However, designing optimal SSR markers from bulk sequence data is a laborious and time-consuming process. Results SAT (SSR Analysis Tool is a user-friendly Web application developed to minimize tedious manual operations and reduce errors. This tool facilitates the integration, analysis and display of sequence data from SSR-enriched libraries. SAT is designed to successively perform base calling and quality evaluation of chromatograms, eliminate cloning vector, adaptors and low quality sequences, detect chimera or partially digested sequences, search for SSR motifs, cluster and assemble the redundant sequences, and design SSR primer pairs. An additional virtual PCR step establishes primer specificity. Users may modify the different parameters of each step of the SAT analysis. Although certain steps are compulsory, such as SSR motifs search and sequence assembly, users do not have to run the entire pipeline, and they can choose selectively which steps to perform. A database allows users to store and query results, and to redo individual steps of the workflow. Conclusion The SAT Web application is available at http://sat.cirad.fr/sat, and a standalone command-line version is also freely downloadable. Users must send an email to the SAT administrator tropgene@cirad.fr to request a login and password.

  6. Molecular characterization of indigenous olive genotypes based on SSR analysis

    Directory of Open Access Journals (Sweden)

    Unver Hulya

    2016-01-01

    Full Text Available Trees of 25 widely grown olive genotypes were analyzed using a set of 10 SSR (simple sequence repeat primer pairs and to evaluate genetic diversity and reveal inter-cultivar relationships. Two well-known international olive cultivars (Chetoni and Manzanilla and four widely grown Turkish standard cultivars (Aycalik, Edincik Su, Gemlik, Kilis Yaglik are also included in the study to compare Kilis genotypes. The 10 polymorphic SSR loci exhibited 4 (UDO4 to 17 alleles (UDO43, with expected heterozygozity (He ranging from 0.510 to 0.887 and a mean of 0.692 presenting high polymorphism. In this study we did not determine identical genotypes and Polateli4 and Kilis Yağlık (0.75, Polateli3 and Polateli7 (0.75 and Polateli6 and Manzanilla (0.70 revealed the highest similarity ratio each other. The most genetically divergent cultivars were Elbeyli8 and Musabeyli5 (0.10; Elbeyli3 and Musabeyli7 (0.15 and Musabeyli6 and Elbeyli7 (0.15, respectively.

  7. SSR markers in characterization of sweet corn inbred lines

    Directory of Open Access Journals (Sweden)

    Srdić Jelena

    2008-01-01

    Full Text Available Sweet corn differs from field corn in many important traits. So its breeding although includes some standard procedures demand application of techniques that are important for determining special traits, all because of the specificity of its usage. Application of molecular markers becomes almost a necessity for the breeding of sweet corn, especially because this is the type of maize in which still no definitive heterotic patterns have been determined. So getting to know genetic divergence of the sweet corn inbred lines is of great importance for its breeding. In this paper we analyzed genetic similarity of six sweet corn inbreds based on SSR markers. 40 SSR primers were used in DNA amplification. Results were compared and correlated with the data on specific combining ability, obtained by the diallel analysis. The results of SCA were in concurrence with genetic similarity. Values of rank correlation coefficient were negative, indicating that more similar inbred lines had smaller estimates of SCA, and lines that were less similar had higher estimates of SCA. Rank correlation coefficient between SCA and GS according to Dice coefficient was between -0,16 and -0,57*.

  8. Molecular Characterization of Wheat Genotypes Using SSR Markers

    Directory of Open Access Journals (Sweden)

    Sheikh Arslan Sehgal

    2012-07-01

    Full Text Available Wheat (Triticum aestivum L. rusts are the most destructive and widespread among all other diseases of wheat because of their wide distribution, and their capacity to form new races that can attack previously resistant cultivars which result in serious yield losses. The molecular characterization and genetic diversity of 20 wheat genotypes was investigated using 34 polymorphic Simple Sequence Repeats (SSR screened primers. About thirty-one loci were found. Lr-19 gene was present in all 20 wheat genotypes that cause resistance against wheat rust. Shalimar-86 and Chakwal-86 showed the highest genetic diversity with SH-02 and Ufaq respectively, giving a 98.94% genetic similarity and a minimum genetic diversity was observed between Chakwal-50 and Bhakar which showed that they are 74% similar. The current research found that SSR makers could distinguish and characterize all of the genotypes, more screened primers could be used for study and for saturation of different regions in further research. The identification of rust resistant genes in Pakistani wheat germplasm will help in accelerating the breeding program in future, including pyramiding of different wheat resistant genes in wheat genotypes and varieties.

  9. Noor eesti teater ja Noor-Eesti. Young Estonian Theatre and Young Estonia

    Directory of Open Access Journals (Sweden)

    Katri Aaslav-Tepandi

    2012-04-01

    Full Text Available This article begins by examining points of intersection between two professional theatres, ”Estonia” and ”Vanemuine” (both established in 1906, their young directors – Karl Menning, Paul Pinna, Theodor Altermann, and Karl Jungholz, and the literary movement Young Estonia. Subsequently, we will consider Young Estonia’s theatrical ideals and the influence of these ideas on later Estonian theatrical life. Since not much information has survived regarding direct personal contacts between ”movers and shakers” in the theatre world and Young Estonians, the main focus here shall be on indirect creative connections and influences. One such context is education: like the Young Estonians, theatre activists of the younger generation aspired to place themselves on the larger map of European culture. Thus, their artistic beliefs and goals shall be examined in relation to those of Young Estonians’ quest for modern culture. Pinna, Altermann, Menning, Jungholz, and others went on study tours to Germany and France, where they were energized and inspired by innovative German and Russian theatres, by naturalistic staging, and by psychological realism, both in acting and in performance style. Among their models were A. Antoine’s Théâtre- Libre in Paris, K. Stanislavski’s Art Theatre in Moscow, O. Brahm’s Lessing-Theater, and M. Reinhardt’s Deutsches Theater in Berlin. These models were likewise known to the Young Estonians, but if theatre activists oriented themselves more fundamentally to German naturalist and realist dramatic art, Young Estonians were more taken with ”theatrical theatre” with its symbolist and impressionist influences. The Young Estonians attended performances at both theatres, ”Vanemuine” and ”Estonia”, and wrote numerous theatre reviews. Yet in the Young Estonia albums (yearbooks and in the magazine Young Estonia, theatre topics have a relatively modest representation. Young Estonians did not have direct

  10. The State, the Museum and the Ethnographer in Constructing National Heritage: Defining Estonian National Costumes in the 1930s

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    Marleen Nõmmela

    2011-03-01

    Full Text Available In this article I attempt to analyse the way in which the Estonian national costume, as heritage, was defined through the cooperation of the state, the museum and ethnographers in the 1930s. The nationalist state wished to strengthen the national identity of Estonia. The Estonian National Museum (ENM as a repository of memory and knowledge availed its resources to support cultural propaganda. The ethnographer Helmi Kurrik, a woman of strong will and keen interest in folk textiles, managed to fulfil her obligation at the expense of her own health. The primary result of her labours was a handbook entitled Eesti rahvarõivad (Estonian Folk Costumes (1938 which has influenced general knowledge of folk costumes in Estonia up to the present day – the ‘right’ national costumes are believed to derive from authentic ethnographical folk costumes held in the Estonian National Museum.

  11. Väärikas Estonian Air jäi kampaaniaga hätta / Alyona Stadnik

    Index Scriptorium Estoniae

    Stadnik, Alyona

    2010-01-01

    Estonian Air müüs veebikaupluse cherry.ee kaudu soodsaid kinkekaarte. Vastuolu tekkis asjaolust, et nõudlus kinkekaartide järele oli suurem kui pakkumine. Lennukompanii katkestas soodsate kinkekaartide müügi

  12. [Encapsulated voices : Estonian sound recordings from the German prisoner-of-war camps in 1916-1918] / Tõnu Tannberg

    Index Scriptorium Estoniae

    Tannberg, Tõnu, 1961-

    2013-01-01

    Arvustus: Encapsulated voices : Estonian sound recordings from the German prisoner-of-war camps in 1916-1918 (Das Baltikum in Geschichte und Gegenwart, 5). Hrsg. von Jaan Ross. Böhlau Verlag. Köln, Weimar und Wien 2012

  13. Estonian Business Schooli magistriharidus nüüd ka koju kätte / Madis Habakuk

    Index Scriptorium Estoniae

    Habakuk, Madis

    2007-01-01

    Sügisest hakkab Estonian Business School koos Mainori Kõrgkooliga pakkuma magistriõpet majandushariduseta inimestele, kus soovijatel on võimalus õppida EBSi Master of Business Administration programmi järgi

  14. Kanada rahastaja tõi Estonian Airi ostu lähemale / Rivo Sarapik ; kommenteerinud Andrus Ansip

    Index Scriptorium Estoniae

    Sarapik, Rivo, 1981-

    2010-01-01

    Estonian Air ja Bombardier sõlmisid lennukite hanke lepingu, tingimused ja hind jäid samaks, muutus vaid lennukite tarneaeg. Ostu rahastab Kanada finantseerimisasutus. Uued lennukid saabuvad Eestisse 2011. a. jaanuaris

  15. Use of SSR markers for DNA fingerprinting and diversity analysis of Pakistani sugarcane (Saccharum spp. hybrids) cultivars

    Science.gov (United States)

    In recent years SSR markers have been used widely for genetic analysis. The objective of this study was to use an SSR-based marker system to develop the molecular fingerprints and analyze the genetic relationship of sugarcane cultivars grown in Pakistan. Twenty-one highly polymorphic SSR markers wer...

  16. Health impact assessment of particulate pollution in Tallinn using fine spatial resolution and modeling techniques

    Directory of Open Access Journals (Sweden)

    Kimmel Veljo

    2009-03-01

    Full Text Available Abstract Background Health impact assessments (HIA use information on exposure, baseline mortality/morbidity and exposure-response functions from epidemiological studies in order to quantify the health impacts of existing situations and/or alternative scenarios. The aim of this study was to improve HIA methods for air pollution studies in situations where exposures can be estimated using GIS with high spatial resolution and dispersion modeling approaches. Methods Tallinn was divided into 84 sections according to neighborhoods, with a total population of approx. 390 000 persons. Actual baseline rates for total mortality and hospitalization with cardiovascular and respiratory diagnosis were identified. The exposure to fine particles (PM2.5 from local emissions was defined as the modeled annual levels. The model validation and morbidity assessment were based on 2006 PM10 or PM2.5 levels at 3 monitoring stations. The exposure-response coefficients used were for total mortality 6.2% (95% CI 1.6–11% per 10 μg/m3 increase of annual mean PM2.5 concentration and for the assessment of respiratory and cardiovascular hospitalizations 1.14% (95% CI 0.62–1.67% and 0.73% (95% CI 0.47–0.93% per 10 μg/m3 increase of PM10. The direct costs related to morbidity were calculated according to hospital treatment expenses in 2005 and the cost of premature deaths using the concept of Value of Life Year (VOLY. Results The annual population-weighted-modeled exposure to locally emitted PM2.5 in Tallinn was 11.6 μg/m3. Our analysis showed that it corresponds to 296 (95% CI 76528 premature deaths resulting in 3859 (95% CI 10236636 Years of Life Lost (YLL per year. The average decrease in life-expectancy at birth per resident of Tallinn was estimated to be 0.64 (95% CI 0.17–1.10 years. While in the polluted city centre this may reach 1.17 years, in the least polluted neighborhoods it remains between 0.1 and 0.3 years. When dividing the YLL by the number of

  17. Testing the Visual Soil Assessment tool on Estonian farm fields

    Science.gov (United States)

    Reintam, Endla; Are, Mihkel; Selge, Are

    2017-04-01

    Soil quality estimation plays important role in decision making on farm as well on policy level. Sustaining the production ability and good health of the soil the chemical, physical and biological indicators should be taken into account. The system to use soil chemical parameters is usually quite well established in most European counties, including Estonia. However, measuring soil physical properties, such bulk density, porosity, penetration resistance, structural stability ect is time consuming, needs special tools and is highly weather dependent. In that reason these parameters are excluded from controllable quality parameters in policy in Estonia. Within the project "Interactive Soil Quality Assessment in Europe and China for Agricultural Productivity and Environmental Resilience" (iSQAPER) the visual soil assessment (VSA) tool was developed for easy detection of soil quality as well the different soil friendly agricultural management practices (AMP) were detected. The aim of current study was to test the VSA tool on Estonian farm fields under different management practices and compare the results with laboratory measurements. The main focus was set on soil physical parameters. Next to the VSA, the undisturbed soil samples were collected from the depth of 5-10 cm and 25-30 cm. The study revealed that results of a visually assessed soil physical parameters, such a soil structure, soil structural stability, soil porosity, presence of tillage pan, were confirmed by laboratory measurements in most cases. Soil water stable structure measurement on field (on 1 cm2 net in one 1 l box with 4-6 cm air dry clods for 5-10 min) underestimated very well structured soil on grassland and overestimated the structure aggregates stability of compacted soil. The slightly better soil quality was detected under no-tillage compared to ploughed soils. However, the ploughed soil got higher quality points compared with minimum tillage. The slurry application (organic manuring) had

  18. Characterization and development of EST-SSR markers in sweet potato (Ipomoea batatas (L.) Lam).

    Science.gov (United States)

    Kim, Jin-Hee; Kim, Jun-Hoi; Jo, Won-Sam; Ham, Jeong-Gwan; Chung, Il Kyung; Kim, Kyung-Min

    2016-12-01

    In this study, a cDNA library was constructed from the total RNA of sweet potato leaves. A total of 789 copies of the cDNA were cloned in Escherichia coli by employing the pGEM-T Easy vector. Sequencing was carried out by Solgent Co. (Korea). As many as 579 expressed sequence tag-simple sequence repeat (EST-SSR) markers were designed (73.38%) from the known cDNA nucleotide base sequences. The lengths of the developed EST-SSR markers ranged from 100 to 499 bp (average length 238 bp). Their motif sequence types were varied, with most being dinucleotides and pentanucleotides, and the most commonly found motifs were CAGAAT (29.0%) and TCT (2.8%). Based on these SSR-containing sequences, 619 pairs of high-quality SSR primers were designed using WebSat and Primer3web. The total number of primers designed was 144. Polymorphism was evident in 82 EST-SSR markers among 20 Korean sweet potato cultivars tested and in 90 EST-SSR markers in the two parents of a mapping population, Yeseumi and Annobeny. In this study, the hexaploid sweet potato (2n = 6x = 90) EST-SSR markers were developed in the absence of full-sequence data. Moreover, by acting as a molecular tag for particular traits, the EST-SSR marker can also simultaneously identify information about the corresponding gene. These EST-SSR markers will allow the molecular analysis of sweet potato to be done more efficiently. Thus, we can develop high-quality sweet potato while overcoming the challenges from climate change and other unfavorable conditions.

  19. Efficiency of biparental crossing in sugarcane analyzed by SSR markers

    Directory of Open Access Journals (Sweden)

    João Messias dos Santos

    2014-07-01

    Full Text Available Sugarcane has hermaphrodite flowers, however, selfing and cross pollination may occur, resulting in selfed or hybrid progeny. The aim of this study was to analyze the paternity of progenies from biparental crosses, in order to identify true hybrids or progenies originating from pollen of unknown origin. Seventy-six progenies from four crosses were analyzed using three highly polymorphic microsatellite markers (SSR. Progenies showed moderate genetic similarity and were grouped into four distinct groups, according to the crosses. Transmission of alleles from parents to offspring was clearly observed, in which selfed individuals were not observed, and only true hybrids or progeny resulting from fertilization with pollen uncommon to both parents were. Results showed that there was contamination with pollen from unknown parents in sugarcane crosses, suggesting that errors in the pedigree may occur, and adjustment in the crossing procedure would decrease progenies from pollen of unknown origin.

  20. A population genetic study in the Ochamchir region, Abkhazia, SSR.

    Science.gov (United States)

    Ferrell, R E; Salamatina, N V; Dalakishvili, S M; Bakuradze, N A; Chakraborty, R

    1985-01-01

    The reported longevity of residents of the Soviet Socialist Republic of the Caucasus has focused considerable attention on this population. However, little is known of the genetic composition of this population. With this in mind, several village populations of the Ochamchir Region, Abkhazia, SSR, were typed for 37 discrete genetic blood groups, erythrocyte and plasma protein loci. Gene and haplotype frequencies calculated for the polymorphic markers were determined and the results used in an analysis of intervillage heterogeneity and genetic distance analysis comparing the Abkhazians to European and Asian reference populations. The Abkhazians are approximately equal distance from European and West Asian populations in a genetic sense, and this is consistent with their geographical location. In addition to the usual genetic polymorphisms, rare electrophoretic variants were encountered at the lactate dehydrogenase A and phosphohexose isomerase loci. These results suggest that the population of the Ochamchir Region is relatively homogeneous and not distinctly different from its geographical neighbors.

  1. Genetic variability assessment in the genus Passiflora by SSR markers

    Directory of Open Access Journals (Sweden)

    Claudia Lougon Paiva

    2014-09-01

    Full Text Available The genus Passiflora encompasses many species that are endemic to the Brazilian territory, including some with economic value. Studies on genetic diversity in this genus are fundamental because they allow understanding genetic variability and distance. The present study aimed to determine the genetic variability and distances among 10 species of the genus Passiflora by using microsatellite markers (Simple Sequence Repeat, SSR. Twenty-eight heterologous microsatellite markers were tested, but only 12 were used in the diversity analysis because they amplified in at least 80% of the species. A clear separation was observed among the subgenuses studied, as well as wide variation among the accessions of Passiflora. This knowledge enables breeders to explore diversity and transfer favorable alleles found in wild species.

  2. Genetic Diversity of Aromatic Rice Germplasm Revealed By SSR Markers

    Directory of Open Access Journals (Sweden)

    Saba Jasim Aljumaili

    2018-01-01

    Full Text Available Aromatic rice cultivars constitute a small but special group of rice and are considered the best in terms of quality and aroma. Aroma is one of the most significant quality traits of rice, and variety with aroma has a higher price in the market. This research was carried out to study the genetic diversity among the 50 aromatic rice accessions from three regions (Peninsular Malaysia, Sabah, and Sarawak with 3 released varieties as a control using the 32 simple sequence repeat (SSR markers. The objectives of this research were to quantify the genetic divergence of aromatic rice accessions using SSR markers and to identify the potential accessions for introgression into the existing rice breeding program. Genetic diversity index among the three populations such as Shannon information index (I ranged from 0.25 in control to 0.98 in Sabah population. The mean numbers of effective alleles and Shannon’s information index were 0.36 and 64.90%, respectively. Similarly, the allelic diversity was very high with mean expected heterozygosity (He of 0.60 and mean Nei’s gene diversity index of 0.36. The dendrogram based on UPGMA and Nei’s genetic distance classified the 53 rice accessions into 10 clusters. Analysis of molecular variance (AMOVA revealed that 89% of the total variation observed in this germplasm came from within the populations, while 11% of the variation emanated among the populations. These results reflect the high genetic differentiation existing in this aromatic rice germplasm. Using all these criteria and indices, seven accessions (Acc9993, Acc6288, Acc6893, Acc7580, Acc6009, Acc9956, and Acc11816 from three populations have been identified and selected for further evaluation before introgression into the existing breeding program and for future aromatic rice varietal development.

  3. Data of 10 SSR markers for genomes of homo sapiens and monkeys.

    Science.gov (United States)

    Reddy, K K V V V S; Raju, S Viswanadha; Someswara Rao, Chinta

    2017-06-01

    In this data, we present 10 Simple Sequence Repeat(SSR) markers TAGA, TCAT, GAAT, AGAT, AGAA, GATA, TATC, CTTT, TCTG and TCTA which are extracted from the genomes of homo sapiens and monkeys using string matching mechanism [1]. All loci showed 4 Base Pair(bp) in allele size, indicating that there are some polymorphisms between individuals correlating to the number of SSR repeats that maybe useful for the detection of similarity among the genotypes. Collectively, these data show that the SSR extraction is a valuable method to illustrate genetic variation of genomes.

  4. Strategies for Estonian rural family enterprises. Eesti maapiirkonna pereettevõtete strateegia

    Directory of Open Access Journals (Sweden)

    Maret Kirsipuu

    2013-01-01

    Full Text Available The paper seeks to analyse family businesses in rural areas, family business strategies and re-registration of sole proprietors with the Centre of Registers and Information Systems (hereinafter Commercial Register in 2009, and to provide an overview of entrepreneurship policies targeted at Estonian rural businesses. Layoffs have increased the number of unemployed; some of those who have lost employment opt for social assistance benefits, but some others decide to become entrepreneurs. Many enterprising people in Estonia have set up a family enterprise, mainly in the sphere of services, agriculture and tourism. The Estonian entrepreneurship policy supports enterprising people and approves of entrepreneurship as a promoter of national economic development. One of the most positive qualities of family enterprises is their short decision-making chain, which ensures rapid implementation of the strategy.

  5. The Role of Language in (Recreating Tatar Diaspora Identity: The Case of the Estonian Tatars

    Directory of Open Access Journals (Sweden)

    Maarja Klaas

    2015-06-01

    Full Text Available This paper focuses on the meanings assigned to Tatar language among the Tatar diaspora in Estonia. According to interviews with Estonian Tatars as well as descriptions of field material from Tatarstan, language is an important aspect of Tatar ethnic identity. This paper will track common discourses about the Tatar language and the way it is connected to Tatar ethnic identity. Issues concerning Tatar language are used to demonstrate various ways of enacting Tatarness in Estonia. It is shown that Estonian Tatars worry about the vitality and purity of Tatar language, but for some, marginalization of dialects is also an issue. People categorized with the same identity labels by self and others can experience and enact their Tatarness in a variety of different ways.

  6. The Challenges Organic Food Processors Meet at Small Emerging Market – Estonian Case

    OpenAIRE

    Sarapuu, Kerttu; Pehme, Sirli; Peetsmann, Elen; Matt, Darja

    2014-01-01

    Organic farming and demand for organic products is continually a growing trend all over the world (Willer et al., 2013). In Estonia the share of organic land is 15% of all agricultural land and the number of organic farmers is also growing (Vetemaa, Mikk 2013). Estonian organic food market is still in forming stage being affected by local organic farming development, marketing situation, economic situation and consumer attitudes. Organic processing has clearly not kept up with organic farming...

  7. Market Opportunities for Automotive Components Used in Non-automotive Applications Manufacturing : Estonian Industrial Market

    OpenAIRE

    Kalajärv, Jaana

    2012-01-01

    This thesis examines the opportunities available for automotive components in the Esto-nian non-automotive industrial market. The literature review part is mainly covers the theory of marketing research, because of the approach chosen for this paper. Only small part of marketing research process is re-searched closer in literature review section, because of the scope of the research. Three most relevant steps for the thesis are chosen from research process for further more in-vestigation,...

  8. Short outlines of books by Estonian authors : [annotations] / Rutt Hinrikus, Janika Kronberg

    Index Scriptorium Estoniae

    Hinrikus, Rutt, 1946-

    1998-01-01

    Laretei, Käbi. Eksiil; Toona, Elin. Lotukata; Park, Eeva. Naeru õpilane; Luik, Viivi. Inimese kapike; Laaman, Ilona. Vesi ahjus; Viiding, Juhan (Üdi, Jüri). Kogutud luuletused; Paju, Juhan. Katkenud romaan; Paju, Juhan. Hõõguv rist; Estonian short stories / toim. Kajar Pruul ja Darlene Reddaway; Traat, Mats. Kartaago kiirrong; Kauksi Ülle. Säng; Kross, Jaan. Paigallend; Puhvel, Madli. Symbol of dawn; Kaplinski, Jaan. Võimaluste võimalikkus; Kaplinski, Jaan. Usk on uskmatus

  9. Serological evidence of exposure to globally relevant zoonotic parasites in the Estonian population

    DEFF Research Database (Denmark)

    Lassen, Brian; Janson, Marilin; Viltrop, Arvo

    2016-01-01

    We investigated Estonian population and its selected subgroups for serological evidence of exposure to Ascaris lumbricoides, Echinococcus spp., Taenia solium, Toxocara canis, Toxoplasma gondii, and Trichinella spiralis. Serum samples from 999 adults representing general population, 248 children...... spp. seroprevalence was higher in children. By contrast, T. gondii seroprevalence was higher in animal caretakers, and lower in children, than in the general population. In the general population, the WB-confirmed Echinococcus spp. seroprevalence was 0.5%, T. solium cysticercosis seroprevalence was 0...

  10. Level of knowledge and sources of information about the rheumatoid arthritis in Estonian patients.

    Science.gov (United States)

    Põlluste, Kaja; Kallikorm, Riina; Lember, Margus

    2014-05-01

    The objective of this paper was to find out how many patients with rheumatoid arthritis (RA) know about several aspects of disease, to explain the associations between the level of self-rated knowledge and patients' background and health status and to compare the importance of the main sources of information. A random sample (n = 1,259) of adult Estonian RA patients was selected from the Estonian Health Insurance Fund Database. The patients completed a self-administered questionnaire, which included information about their socio-demographic and disease characteristics, use of health services, information about the disease, and sources of information. Regression analysis was used to calculate the associations between the independent variables and level of self-rated knowledge about several aspects of RA. The results of the study indicated that the self-reported ratings of knowledge about the disease in Estonian RA patients were rather low. Health professionals were mentioned as the primary sources of information, but the longer disease history and more frequent use of health services as considerable predictors of higher ratings of knowledge refer to role of personal experience in obtaining knowledge about the disease as well.

  11. Cancer incidence and thyroid disease among Estonian Chernobyl clean-up workers

    International Nuclear Information System (INIS)

    Auvinen, A.; Salomaa, S.; Boice, J.D. Jr.

    1998-09-01

    The report describes the development and summarizes the results of the project Cancer incidence and thyroid disease among Estonian Chernobyl clean-up workers. One of the goals of the report is to give research protocols and questionnaires for researchers involved in other studies. Eight previously published articles are also included summarizing the results. The development of the collaboration work of the project is described in the introduction of the report. Epidemiological methods are described in an article complemented by the protocol and English version of the questionnaire administered to all cleanup workers, as well as the data collection form of the thyroid study. The results from biological biodosimetry using both glycophorin A and FISH methods have shown that the radiation doses received by the Chernobyl cleanup workers were relatively low. Thyroid nodularity was not associated with any radiation exposure characteristic in the thyroid screening study. Estonian Chernobyl cleanup workers were followed up for cancer incidence through the Estonian Cancer Registry. No cases of leukemia or thyroid cancer were observed by the end of 1993. It is too early to observe possible effect on other types of cancer. However, mortality from suicides was increased compared with general population. Further follow-up and the extension to other Baltic countries in the future will undoubtedly strengthen the study. There are also plans for future projects covering areas from psychosocial factors to radiation biology

  12. DNA Profiles of MTG (Moderat Tahan Gano) Oil Palm Variety Based on SSR Marker

    Science.gov (United States)

    Putri, L. A. P.; Setiado, H.; Hardianti, R.

    2017-03-01

    The oil palm, an economically important tree in Indonesia, has been one of the world’s major sources of edible oil and a significant precursor of biodiesel fuel. The objectives of this study were to know DNA profile of commercial MTG (Moderat Tahan Gano) oil palm variety collections. A total of 10 trees MTG oil palm variety were used for analysis. In this experiment, the DNA profile diversity was assessed using mEgCIR0174 and SSR-1 loci of oil palm’s specific SSR markers. The results of the experiment indicated out of 3 alleles of pcr product of mEgCIR0174 (198, 203 and 208 bp) and SSR-1 (201, 217 and 232 bp). These preliminary results demonstrated SSR marker can be used to evaluate genetic relatedness among trees of MTG (Moderat Tahan Gano) oil palm variety derived from different crossing or difference to desease resistance trait or misslabeled.

  13. Supplementary Controller Design for SSR Damping in a Series-Compensated DFIG-Based Wind Farm

    Directory of Open Access Journals (Sweden)

    Minqiang Hu

    2012-11-01

    Full Text Available The increasing presence of wind power in power systems will likely drive the integration of large wind farms with electrical networks that are series-compensated to sustain large power flows. This may potentially lead to subsynchronous resonance (SSR issues. In this paper, a supplementary controller on the grid-side converter (GSC control loop is designed to mitigate SSR for wind power systems based on doubly fed induction generators (DFIGs with back-to-back converters. Different supplementary controller feedback signals and modulated-voltage injecting points are proposed and compared based on modal analysis and verified through root locus analysis to identify the optimal feedback signal and the most effective control location for SSR damping. The validity and effectiveness of the proposed supplemental control are demonstrated on the IEEE first benchmark model for computer simulations of SSR by means of time domain simulation analysis using Matlab/Simulink.

  14. Cranberry SSR multiplexing panels for DNA horticultural fingerprinting and genetic studies

    Science.gov (United States)

    Cranberry (Vaccinium macrocarpon) is in need of inexpensive high-throughput DNA fingerprinting methods for genetic research and germplasm purity testing for agricultural purposes. Therefore, we designed and validated 16-multiplexing panels containing 61 evenly distributed simple sequence (SSR) marke...

  15. Impedance characteristics of DFIGs considering the impacts of DFIG numbers and its application on SSR analysis

    Science.gov (United States)

    You, J. J.; Ning, W. Y.; Jiang, H.; Dong, Y. X.; Liu, H.; Li, Y.

    2017-05-01

    Sub-synchronous resonance (SSR) occurred in the power system with both Doubly Fed Induction Generators (DFIGs) and series-compensated lines. There have been several papers focusing on the analysis of such SSR phenomenon. To the authors’ knowledge, the impacts of DFIG numbers are seldom considered in existing papers. In this paper, the impedance characteristics of DFIGs under different numbers are analyzed. Then the relationship between DFIG numbers and SSR characteristics is discussed. The contributions to be included in the paper are as following: 1) the impedance of N DFIGs is roughly equal to 1/N of the impedance of one DFIG. 2) The SSR risk of system is related with both the numbers of DFIGs and the parameters of the series-compensated lines.

  16. Regulation of Transcription from Two ssrS Promoters in 6S RNA Biogenesis

    Science.gov (United States)

    Lee, Ji Young; Park, Hongmarn; Bak, Geunu; Kim, Kwang-sun; Lee, Younghoon

    2013-01-01

    ssrS-encoded 6S RNA is an abundant noncoding RNA that binds σ70-RNA polymerase and regulates expression at a subset of promoters in Escherichia coli. It is transcribed from two tandem promoters, ssrS P1 and ssrS P2. Regulation of transcription from two ssrS promoters in 6S RNA biogenesis was examined. Both P1 and P2 were growth phase-dependently regulated. Depletion of 6S RNA had no effect on growth-phase-dependent transcription from either promoter, whereas overexpression of 6S RNA increased P1 transcription and decreased P2 transcription, suggesting that transcription from P1 and P2 is subject to feedback activation and feedback inhibition, respectively. This feedback regulation disappeared in Δfis strains, supporting involvement of Fis in this process. The differential feedback regulation may provide a means for maintaining appropriate cellular concentrations of 6S RNA. PMID:23864284

  17. SSR genetic linkage map construction of pea (Pisum sativum L. based on Chinese native varieties

    Directory of Open Access Journals (Sweden)

    Xuelian Sun

    2014-04-01

    Full Text Available Simple sequence repeat (SSR markers have previously been applied to linkage mapping of the pea (Pisum sativum L. genome. However, the transferability of existing loci to the molecularly distinct Chinese winter pea gene pool was limited. A novel set of pea SSR markers was accordingly developed. Together with existing SSR sequences, the genome of the G0003973 (winter hardy × G0005527 (cold sensitive cross was mapped using 190 F2 individuals. In total, 157 SSR markers were placed in 11 linkage groups with an average interval of 9.7 cM and total coverage of 1518 cM. The novel markers and genetic linkage map will be useful for marker-assisted pea breeding.

  18. Genetic diversity of wild and cultivated Rubus species in Colombia using AFLP and SSR markers

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    Sandra Bibiana Aguilar

    2007-01-01

    Full Text Available The Andean blackberry belongs to the genus Rubus, the largest of the Rosaceae family and one of the mostdiverse of the plant kingdom. In Colombia Rubus glaucus Benth, known as the Andean raspberry or blackberry, is one of thenine edible of the genus out of forty-four reported species. In this study wild and cultivated genotypes, collected in the CentralAndes of Colombia were analyzed by AFLP and SSR markers. Sexual reproduction seems to play an important role inmaintaining the genetic variability in R. glaucus, and the viability of using the SSR of Rubus alceifolius to characterizeColombian Rubus species was clearly demonstrated. All species evaluated produced very specific banding patterns,differentiating them from the others. Both AFLP and SSR produced bands exclusive to each of the following species: R.robustus, R. urticifolius, R. glaucus, and R. rosifolius. The SSR markers differentiated diploid and tetraploid genotypes of R.glaucus.

  19. Assessment of Multiple GNSS Real-Time SSR Products from Different Analysis Centers

    Directory of Open Access Journals (Sweden)

    Zhiyu Wang

    2018-03-01

    Full Text Available The real-time State Space Representation (SSR product of the GNSS (Global Navigation Satellite System orbit and clock is one of the most essential corrections for real-time precise point positioning (PPP. In this work, the performance of current SSR products from eight analysis centers were assessed by comparing it with the final product and the accuracy of real-time PPP. Numerical results showed that (1 the accuracies of the GPS SSR product were better than 8 cm for the satellite orbit and 0.3 ns for the satellite clock; (2 the accuracies of the GLONASS (GLObalnaya NAvigatsionnaya Sputnikovaya Sistema SSR product were better than 10 cm for orbit RMS (Root Mean Square and 0.6 ns for clock STD (Standard Deviation; and (3 the accuracies of the BDS (BeiDou Navigation Satellite System and Galileo SSR products from CLK93 were about 14.54 and 4.42 cm for the orbit RMS and 0.32 and 0.18 ns for the clock STD, respectively. The simulated kinematic PPP results obtained using the SSR products from CLK93 and CLK51 performed better than those using other SSR products; and the accuracy of PPP based on all products was better than 6 and 10 cm in the horizontal and vertical directions, respectively. The real-time kinematic PPP experiment carried out in Beijing, Tianjin, and Shijiazhuang, China indicated that the SSR product CLK93 from Centre National d’Etudes Spatiales (CNES had a better performance than CAS01. Moreover, the PPP with GPS + BDS dual systems had a higher accuracy than those with only a GPS single system.

  20. Development of genomic SSR markers for fingerprinting lettuce (Lactuca sativa L. cultivars and mapping genes

    Directory of Open Access Journals (Sweden)

    Rauscher Gilda

    2013-01-01

    Full Text Available Abstract Background Lettuce (Lactuca sativa L. is the major crop from the group of leafy vegetables. Several types of molecular markers were developed that are effectively used in lettuce breeding and genetic studies. However only a very limited number of microsattelite-based markers are publicly available. We have employed the method of enriched microsatellite libraries to develop 97 genomic SSR markers. Results Testing of newly developed markers on a set of 36 Lactuca accession (33 L. sativa, and one of each L. serriola L., L. saligna L., and L. virosa L. revealed that both the genetic heterozygosity (UHe = 0.56 and the number of loci per SSR (Na = 5.50 are significantly higher for genomic SSR markers than for previously developed EST-based SSR markers (UHe = 0.32, Na = 3.56. Fifty-four genomic SSR markers were placed on the molecular linkage map of lettuce. Distribution of markers in the genome appeared to be random, with the exception of possible cluster on linkage group 6. Any combination of 32 genomic SSRs was able to distinguish genotypes of all 36 accessions. Fourteen of newly developed SSR markers originate from fragments with high sequence similarity to resistance gene candidates (RGCs and RGC pseudogenes. Analysis of molecular variance (AMOVA of L. sativa accessions showed that approximately 3% of genetic diversity was within accessions, 79% among accessions, and 18% among horticultural types. Conclusions The newly developed genomic SSR markers were added to the pool of previously developed EST-SSRs markers. These two types of SSR-based markers provide useful tools for lettuce cultivar fingerprinting, development of integrated molecular linkage maps, and mapping of genes.

  1. Comparative Analysis of Genetic Diversity and Structure in Rice Using ILP and SSR Markers

    Directory of Open Access Journals (Sweden)

    Ming HUANG

    2010-12-01

    Full Text Available Genetic diversity of 36 rice entries from the United States Department of Agriculture (USDA rice collection was assessed using 103 ILP (intron length polymorphism and 54 SSR (simple sequence repeats markers. A total of 236 and 332 alleles were detected by the ILP and SSR markers, respectively. On average, the SSR markers produced higher polymorphism information content value and number of alleles than the ILP markers. Whereas the Nei's genetic distance measured using the SSR markers was much higher than that measured using the ILP markers. Mantel's test indicated that there was a statistically significant correlation (r=0.827, P<0.001 between the two marker systems. UPGMA clustering based on the ILP and SSR markers resulted in consensus dendrograms. The cophenetic correlation coefficient (r=0.918, 0.878 and 0.924, P<0.001 for the ILP, SSR and combined markers, respectively showed a highly accurate dendrogram represented the genetic distance among these entries. The 36 entries were divided into four groups. Four African Oryza glaberrima accessions were clustered within a distinct group (I, and the remaining entries were separated into three groups (II, III and IV. All the entries could be also clustered into two main groups: One was composed of III and IV, considered as indica group, and the other was composed of I (O. glaberrima and II (japonica-like. Model-based cluster analysis revealed that the japonica-like group maintained very pure ancestry while the indica group shared mixed ancestry, especially for group III, which had seven admixtures sharing from 19.5% to 30.0% of ancestry with group IV (based on SSR markers. It is suggested that ILP and SSR markers could be very useful for the genetic study and breeding in rice.

  2. Development of genomic SSR markers for fingerprinting lettuce (Lactuca sativa L.) cultivars and mapping genes.

    Science.gov (United States)

    Rauscher, Gilda; Simko, Ivan

    2013-01-22

    Lettuce (Lactuca sativa L.) is the major crop from the group of leafy vegetables. Several types of molecular markers were developed that are effectively used in lettuce breeding and genetic studies. However only a very limited number of microsattelite-based markers are publicly available. We have employed the method of enriched microsatellite libraries to develop 97 genomic SSR markers. Testing of newly developed markers on a set of 36 Lactuca accession (33 L. sativa, and one of each L. serriola L., L. saligna L., and L. virosa L.) revealed that both the genetic heterozygosity (UHe = 0.56) and the number of loci per SSR (Na = 5.50) are significantly higher for genomic SSR markers than for previously developed EST-based SSR markers (UHe = 0.32, Na = 3.56). Fifty-four genomic SSR markers were placed on the molecular linkage map of lettuce. Distribution of markers in the genome appeared to be random, with the exception of possible cluster on linkage group 6. Any combination of 32 genomic SSRs was able to distinguish genotypes of all 36 accessions. Fourteen of newly developed SSR markers originate from fragments with high sequence similarity to resistance gene candidates (RGCs) and RGC pseudogenes. Analysis of molecular variance (AMOVA) of L. sativa accessions showed that approximately 3% of genetic diversity was within accessions, 79% among accessions, and 18% among horticultural types. The newly developed genomic SSR markers were added to the pool of previously developed EST-SSRs markers. These two types of SSR-based markers provide useful tools for lettuce cultivar fingerprinting, development of integrated molecular linkage maps, and mapping of genes.

  3. Field trial on glucose-induced insulin and metabolite responses in Estonian Holstein and Estonian Red dairy cows in two herds

    Directory of Open Access Journals (Sweden)

    Kaart Tanel

    2010-01-01

    Full Text Available Abstract Background Insulin secretion and tissue sensitivity to insulin is considered to be one of the factors controlling lipid metabolism post partum. The objective of this study was to compare glucose-induced blood insulin and metabolite responses in Estonian Holstein (EH, n = 14 and Estonian Red (ER, n = 14 cows. Methods The study was carried out using the glucose tolerance test (GTT performed at 31 ± 1.9 days post partum during negative energy balance. Blood samples were obtained at -15, -5, 5, 10, 20, 30, 40, 50 and 60 min relative to infusion of 0.15 g/kg BW glucose and analysed for glucose, insulin, triglycerides (TG, non-esterified fatty acids (NEFA, cholesterol and β-hydroxybutyrate (BHB. Applying the MIXED Procedure with the SAS System the basal concentration of cholesterol, and basal concentration and concentrations at post-infusion time points for other metabolites, area under the curve (AUC for glucose and insulin, clearance rate (CR for glucose, and maximum increase from basal concentration for glucose and insulin were compared between breeds. Results There was a breed effect on blood NEFA (P P P P P P th min nadir (P th min postinfusion (P Conclusion Our results imply that glucose-induced changes in insulin concentration and metabolite responses to insulin differ between EH and ER dairy cows.

  4. Role of environment in strengthening competitiveness of cities by example of European Green Capitals and Tallinn

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    Jüri Ratas

    2014-01-01

    Full Text Available Guaranteeing a high-level living environment is one of the most important preconditions to the balanced and sustainable economic and social development of the European Union. Serious environmental problems can be seen in European cities, which are the places where most of the population live and which create the highest number of jobs, economic growth and added value. The level of urbanisation in Europe was 72.7% in 2010 and the UN forecast that it will increase to 82.2% by 2050. The European Green Capital Award was created in 2006 in order to recognise cities that have contributed to the improvement of their quality of life. The cities that have won the award have started using the most innovative and efficient measures for increasing the city’s competitiveness, and are an example of how to achieve sustainable development for all cities, not just capitals. The environmental indicators of cities that have won the European Green Capital Award are high. In this article the problem is analysed using the example of Tallinn, the capital of Estonia, which strives for a place among cities with the highest quality of life in Europe. The objective of this article is to analyse the areas of the city’s environmental activities and environmental organisation in the context of increasing competitiveness. The original database used by the author in this article consists of the responses given by the governments of the cities who have already been awarded the title of European Green Capital when asked to describe the impact that applying for and achieving the title had on their cities

  5. Eesti antiigitõlke traditsioonid / Traditions of Estonian Translation from Ancient Greek and Latin

    Directory of Open Access Journals (Sweden)

    Janika Päll

    2012-06-01

    Full Text Available The tradition of translating ancient Greek and Roman authors into Estonian is short, beginning with first attempts at the end of the 18 th century and the close adaptations of two Anacreontic poems (21 and 24 by the first Estonian poet Kristjan Jaak Peterson (in 1818, which remained in manuscript for a long time. The continuous history of printed translations began in 1878 with the translations from Homer by Jaan Bergmann. At present, a new, extensive and regularly updated bibliography with a database of earlier translations is being created (EAB 2012, which also includes the translations in the journals and more extensive citations in articles, as printing separate books with ancient literature started very late (1908 and was very rare in the beginning.  The periods in Estonian translation reflect the history of the country. Almost every period has its own specific trends, beginning with the focus on Greek and the role of periodicals in the first, resembling the patchwork-model of translation that has been described by Karl Eimermacher. Alongside the wish to entertain and educate, we see a strong tendency to use these translations for the development of Estonian national identity by comparing the Estonian epic “Kalevipoeg” to Homer’s epics and translating pieces from Tacitus’ “Germania” as early references to Estonia and thereby extending Estonia’s written history.  The 1920s and 1930s bring first attempts to create a canon, with a stress on Latin and the translations made for school, as well as the development of verse translation. However, all this was disrupted by the almost total abandonment of the classical tradition during the war and the Stalinist period. The comeback in the 1960s brought the translation of central authors from the classical canon (Homer, Vergil, Sophocles, supported by other activities of canon-building (anticipated partly in the 1920s and 1930s: the composition of anthologies and histories of literature

  6. Transcriptomic analysis, genic SSR development, and genetic diversity of proso millet (Panicum miliaceum; Poaceae).

    Science.gov (United States)

    Hou, Siyu; Sun, Zhaoxia; Li, Yaoshen; Wang, Yijie; Ling, Hubin; Xing, Guofang; Han, Yuanhuai; Li, Hongying

    2017-07-01

    Proso millet ( Panicum miliaceum ; Poaceae) is a minor crop with good nutritional qualities and strong tolerance to drought stress and soil infertility. However, studies on genetic diversity have been limited due to a lack of efficient genetic markers. Illumina sequencing technology was used to generate short read sequences of proso millet, and de novo transcriptome assemblies were used to develop a de novo assembly of proso millet. Genic simple sequence repeat (SSR) markers were identified and used to detect polymorphism among 56 accessions. Population structure and genetic similarity coefficient were estimated. In total, 25,341 unique gene sequences and 4724 SSR loci were obtained from the transcriptome, of which 229 pairs of SSR primers were validated, which resulted in 14 polymorphic genic SSR primers exhibiting 43 total alleles. According to the ratio of polymorphic markers (6.1%, 14/229), there are potentially 288 polymorphic genic SSR markers available for genetic assay development in the future. Bayesian population analyses showed that the 56 accessions comprised two distinct groups. A genetic structure and cluster assay indicated that the accessions from the Loess Plateau of China shared a high genetic similarity coefficient with those from other regions and that there was no correlation between genetic diversity and geographic origin. The transcriptome sequencing data and millet-specific SSR markers developed in this study establish an excellent resource for gene discovery and may improve the development of breeding programs in proso millet in the future.

  7. Genetic diversity analysis of Valencia and Navel group sweet orange cultivars by SSR markers

    Directory of Open Access Journals (Sweden)

    İlknur POLAT

    2015-06-01

    Full Text Available Sweet orange [Citrus sinensis (L. Osbeck] fruit is one of the main citrus fruits, Navel and Valencia group sweet orange being the most representative and recognizable species of this species. The aims of this study were to determine genetic relationships and diversity of 84 Navel and 36 Valencia groups of sweet orange using SSR (simple sequence repeat molecular markers. Twenty-six SSR primers were tested on these accessions. Seven SSR primers produced thirteen polymorphic fragments, eight SSR primers produced monomorphic fragments, and eleven SSR primers produced no scorable fragments. Thirteen SSR primers produced a total of 29 fragments and 13 of them were polymorphic. The number of average polymorphic fragments per primer was 1.93. The mean polymorphism information content (PIC and marker index (MI are 0.16 and 11.74, respectively. The Dice’s similarity coefficient among Navel and Valencia group sweet oranges ranged from 0.42 to 1.00 and matrix correlation (r was 0.79. In the cluster analysis, Navel group sweet oranges were indicated as a separate group from Valencia group sweet oranges. ‘Antalya (40’ was most distinct accessions from the others.

  8. Eesti Vabaõhumuuseumi külastuskeskus - ennistuskoda Kanut - Tallinna muuseumide ühishoidla arhitektuurne ideevõistlus = Estonian Open Air Museum Visitor Centre - Kanut Restoration Centre - Joint Depository for Tallinn museums : an Idea Competitio

    Index Scriptorium Estoniae

    Põdra, Laila, 1967-

    2007-01-01

    Ideekonkursi eesmärgist, I preemia pälvinud tööst "Raudjas" (autorid: Siiri Vallner, Imdrek Peil), ostupreemia pälvinud töödest "Leevike" (autor AB Kosmos: Ott Kadarik, Mihkel Tüür, Villem Tomiste) ja "Vurr" (autorid: Leena Torim, Maia Tüür, Veronika Valk)

  9. Genetic similarity among Tunisian cultivated olive estimated through SSR markers

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    Sofiane Abdelhamid

    2013-02-01

    Full Text Available Olive (Olea europaea L. subsp. europaea var. europaea is one of the oldest fruit tree in the Mediterranean basin, and is cultivated for oil and canned fruit. Part of this interest is driven by the economic importance of olive oil which is increasing throughout the world due to its beneficial effect to human health. In Tunisia, olive has great socio-economic importance, with more than 60 millions olive trees cultivated for olive oil production including a wide range of cultivars which are widely extended from the north to the south regions of the country for its high economic value. Here, we applied microsatellites (SSRs molecular markers to assess the genetic variability of the most important Tunisian olive cultivars. In total, the 10 simple sequence repeats (SSR loci revealed 73 alleles with a mean number of 07 alleles per locus were detected. The polymorphism index content (PIC values were high (0.72 ranging from 0.86 at GAPU 103 to 0.56 at EMO 90. The analysis of the dendrogram showed six main separate groups.

  10. STUDY OF GENETIC VARIABILITY OF TRITICALE VARIETIES BY SSR MARKERS

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    Jana Ondroušková

    2013-04-01

    Full Text Available For the detection of genetic variability ten genotypes of winter triticale (×Triticosecale Wittmack, 2n = 6x = 42; BBAARR were selected: nine varieties and one breeding line with good bread-making quality KM 4-09 with the chromosome translocation 1R.1D 5+10-2. 25 microsatellites markers located in the genome A, B, D and R were chosen for analysis. Eighty-four alleles were detected with an average of 3.36 alleles per locus were detected. For each microsatellite statistical values were calculated diversity index (DI, probabilities of identity (PI and polymorphic information content (PIC were calculated and averages statistical values are: DI 0.55, PI 0.27 and 0.5 PIC. Overall dendrogram based on the UPGMA method (Jaccards similarity coefficient significantly distinguished two groups of genotypes and these groups were divided into sub-clusters. A set of 5 SSR markers (Xwms0752, Xbarc128, Xrems1237, Xwms0861 and Xbrac170 which have the calculated PIC value higher than 0.68 that are sufficient for the identification of the analyzed genotypes was described.

  11. Probabilistic safety assessment for TRIGA SSR 14 MW

    International Nuclear Information System (INIS)

    Mladin, Daniela; Preda, Marin; Mladin, Mirea; Stefan, Ion

    2006-01-01

    Probabilistic Safety Assessment is a method for risk public and personnel evaluation in a complex installation such as a nuclear reactor, a chemical installation, etc. PSA techniques are powerful tools for improvement in design, construction, operation, safety and design efficiency, for modification and management of such complex installations. International practice has shown that safety analysis both for power reactors and research reactors involves definition of postulated initiating events. These initiators depend on reactor type, nominal power, fuel characteristics, safety systems, etc. Such scenarios or accident sequences derived from these initiating events are postulated in some cases, on the basis of engineering judgement. In the others cases, they are developed from probabilistic view point, starting with initiating event frequency and continuing with safety systems failures and human errors, which may intervene in the evolution of the event. Starting from these initiating events, event trees technique allows the calculation of the frequencies of final damage states having environmental or only inside installation consequences, depending on the success or failure of the safety systems analysed separately in the fault trees. The paper presents results of the PSA model for TRIGA SSR 14 Mw reactor, more precisely the qualitative and quantitative analysis of initiating evens and event trees

  12. Genic and Intergenic SSR Database Generation, SNPs Determination and Pathway Annotations, in Date Palm (Phoenix dactylifera L..

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    Morad M Mokhtar

    Full Text Available The present investigation was carried out aiming to use the bioinformatics tools in order to identify and characterize, simple sequence repeats within the third Version of the date palm genome and develop a new SSR primers database. In addition single nucleotide polymorphisms (SNPs that are located within the SSR flanking regions were recognized. Moreover, the pathways for the sequences assigned by SSR primers, the biological functions and gene interaction were determined. A total of 172,075 SSR motifs was identified on date palm genome sequence with a frequency of 450.97 SSRs per Mb. Out of these, 130,014 SSRs (75.6% were located within the intergenic regions with a frequency of 499 SSRs per Mb. While, only 42,061 SSRs (24.4% were located within the genic regions with a frequency of 347.5 SSRs per Mb. A total of 111,403 of SSR primer pairs were designed, that represents 291.9 SSR primers per Mb. Out of the 111,403, only 31,380 SSR primers were in the genic regions, while 80,023 primers were in the intergenic regions. A number of 250,507 SNPs were recognized in 84,172 SSR flanking regions, which represents 75.55% of the total SSR flanking regions. Out of 12,274 genes only 463 genes comprising 896 SSR primers were mapped onto 111 pathways using KEGG data base. The most abundant enzymes were identified in the pathway related to the biosynthesis of antibiotics. We tested 1031 SSR primers using both publicly available date palm genome sequences as templates in the in silico PCR reactions. Concerning in vitro validation, 31 SSR primers among those used in the in silico PCR were synthesized and tested for their ability to detect polymorphism among six Egyptian date palm cultivars. All tested primers have successfully amplified products, but only 18 primers detected polymorphic amplicons among the studied date palm cultivars.

  13. Kodakondsus - kas integratsiooni eeldus või tulemus? / Klara Hallik

    Index Scriptorium Estoniae

    Hallik, Klara

    2000-01-01

    Eestlaste ja mitteeestlaste ootused kodakondsuspoliitika suhtes on jätkuvalt vastandlikud. Ilm. ka: Integration of Estonian Society : monitoring 2000.- Tallinn, 2000 ; Integratsija Estonskogo obshtshestva : monitoring 2000.- Tallinn, 2000

  14. External factors and the interference of L1 Estonian on L2 English pronunciation: An apparent-time study

    OpenAIRE

    Kristiina Ader; Merilin Miljan

    2015-01-01

    This study focusses on external factors of second language learning (L2) and their effect on L2 sound production. The aim was to find out whether young adults whose first language (L1) is Estonian speak L2 English with less accent than older speakers of L1 Estonian. Prior studies have claimed that more exposure to the target language lessens the effect of L1 interference (internal factors) (e.g. Piske et al. 2001, Muñoz, Llanes 2014). An apparent-time study was imple- mented in order to test ...

  15. Koolutatud kirjanduslugu / Jaan Puhvel

    Index Scriptorium Estoniae

    Puhvel, Jaan, 1932-

    2007-01-01

    Arvustus: Nirk, Endel. Estonian literature : historical survey with bibliographical application / [translated from Estonian by A. Hone, O. Mutt]. Tallinn : Perioodika, 1970. Ilmunud: Books Abroad, 1972, nr. 46, lk. 142-144, pealkirjata

  16. Veelahkme väljapurse / Jaan Puhvel

    Index Scriptorium Estoniae

    Puhvel, Jaan, 1932-

    2007-01-01

    Arvustus: Nirk, Endel. Estonian literature : historical survey with bibliographical application / [translated from Estonian by A. Hone, O. Mutt]. Tallinn : Perioodika, 1987. Ilmunud: Los Angeles Times Book Review 19. II 1989, lk. 2, pealkirjata

  17. Noor-Eesti rollist eesti kirjandus- ja kultuuriloos. The Role of Young Estonia in Estonian Literary and Cultural History

    Directory of Open Access Journals (Sweden)

    Sirje Olesk

    2012-04-01

    Full Text Available From the beginning of the 20th century on, the activities of the Young Estonia literary movement have had a significant influence on the development of Estonian culture. The group published five Young Estonia albums (1905–1915, the magazine Young Estonia, devoted to science, literature, and the arts (1910– 1911, and the newspaper Vaba Sõna (1914–1916, Free Word. The core of the group was composed of five or six active writers who maintained lively interactions with art and theatre circles. The publications of the Young Estonia Press brought about a revolutionary turn in the design and printing styles of the Estonian-language book. The questions of Young Estonia’s role and meaning have intrigued the Estonian cultural public at different times and in different ways. Young Estonia brought to cultural consciousness liberal views on the interpretation of art as well as an elitist aesthetics. At different times their openness to Europe irritated both the nationalist-minded and internationalists; thus the reception of the Young Estonia movement has been polemical throughout Estonian cultural history. Elapsed time has both opened the way for broader generalizations and, conversely, created a need to reconstruct the cultural context of the beginning of the 20th century. Polemic around Young Estonia was certainly caused by the group as a whole; however, in this article the role of individual members is also highlighted. It is emphasized that the political and aesthetic ideas of the Young Estonians changed synchronously with social conditions, from the 1905 Russian revolution to the world war that ravaged Europe. If the early part of this period saw a more social and nationalist bent to their thinking, then in the years of reaction, positions were taken that were more in keeping with l’art pour l’art. Young Estonia was not a hierarchical organization; rather, its small core group was composed of a narrow circle of friends with various

  18. Spatial patterns of soil organic carbon stocks in Estonian arable soils

    Science.gov (United States)

    Suuster, Elsa; Astover, Alar; Kõlli, Raimo; Roostalu, Hugo; Reintam, Endla; Penu, Priit

    2010-05-01

    Soil organic carbon (SOC) determines ecosystem functions, influencing soil fertility, soil physical, chemical and biological properties and crop productivity. Therefore the spatial pattern of SOC stocks and its appropriate management is important at various scales. Due to climate change and the contribution of carbon store in the soils, the national estimates of soil carbon stocks should be determined. Estonian soils have been well studied and mapped at a scale 1:10,000. Previous studies have estimated SOC stocks based on combinations of large groups of Estonian soils and the mean values of the soil profile database, but were not embedded into the geo-referenced databases. These studies have estimated SOC stocks of Estonian arable soils 122.3 Tg. Despite of available soil maps and databases, this information is still very poorly used for spatial soil modelling. The aim of current study is to assess and model spatial pattern of SOC stocks of arable soils on a pilot area Tartu County (area 3089 sq km). Estonian digital soil map and soil monitoring databases are providing a good opportunity to assess SOC stocks at various scales. The qualitative nature of the initial data from a soil map prohibits any straightforward use in modelling. Thus we have used several databases to construct models and linkages between soil properties that can be integrated into soil map. First step was to reorganize the soil map database (44,046 mapping units) so it can be used as an input to modelling. Arable areas were distinguished by a field layer of Agricultural Registers and Information Board, which provides precise information of current land use as it is the basis of paying CAP subsidies. The estimates of SOC content were found by using the arable land evaluation database of Tartu from the Estonian Land Board (comprising 950 sq km and 31,226 fields), where each soil type was assessed separately and average SOC content grouped by texture was derived. SOC content of epipedon varies in

  19. Samm välisilma poole / Kaido Jaanson

    Index Scriptorium Estoniae

    Jaanson, Kaido, 1940-

    2003-01-01

    Rets. rmt.: The Estonian Foreign Policy Yearbook 2003. Eesti välispoliitika aastaraamat. Tallinn, 2003 ; Danish Foreign Policy Yearbook 2003. - Copenhagen: Danish Institute for International Studies, 2003

  20. Characterization and development of EST-derived SSR markers in cultivated sweetpotato (Ipomoea batatas).

    Science.gov (United States)

    Wang, Zhangying; Li, Jun; Luo, Zhongxia; Huang, Lifei; Chen, Xinliang; Fang, Boping; Li, Yujun; Chen, Jingyi; Zhang, Xiongjian

    2011-10-20

    Currently there exists a limited availability of genetic marker resources in sweetpotato (Ipomoea batatas), which is hindering genetic research in this species. It is necessary to develop more molecular markers for potential use in sweetpotato genetic research. With the newly developed next generation sequencing technology, large amount of transcribed sequences of sweetpotato have been generated and are available for identifying SSR markers by data mining. In this study, we investigated 181,615 ESTs for the identification and development of SSR markers. In total, 8,294 SSRs were identified from 7,163 SSR-containing unique ESTs. On an average, one SSR was found per 7.1 kb of EST sequence with tri-nucleotide motifs (42.9%) being the most abundant followed by di- (41.2%), tetra- (9.2%), penta- (3.7%) and hexa-nucleotide (3.1%) repeat types. The top five motifs included AG/CT (26.9%), AAG/CTT (13.5%), AT/TA (10.6%), CCG/CGG (5.8%) and AAT/ATT (4.5%). After removing possible duplicate of published EST-SSRs of sweetpotato, a total of non-repeat 7,958 SSR motifs were identified. Based on these SSR-containing sequences, 1,060 pairs of high-quality SSR primers were designed and used for validation of the amplification and assessment of the polymorphism between two parents of one mapping population (E Shu 3 Hao and Guang 2k-30) and eight accessions of cultivated sweetpotatoes. The results showed that 816 primer pairs could yield reproducible and strong amplification products, of which 195 (23.9%) and 342 (41.9%) primer pairs exhibited polymorphism between E Shu 3 Hao and Guang 2k-30 and among the 8 cultivated sweetpotatoes, respectively. This study gives an insight into the frequency, type and distribution of sweetpotato EST-SSRs and demonstrates successful development of EST-SSR markers in cultivated sweetpotato. These EST-SSR markers could enrich the current resource of molecular markers for the sweetpotato community and would be useful for qualitative and quantitative

  1. Characterization and development of EST-derived SSR markers in cultivated sweetpotato (Ipomoea batatas

    Directory of Open Access Journals (Sweden)

    Li Yujun

    2011-10-01

    Full Text Available Abstract Background Currently there exists a limited availability of genetic marker resources in sweetpotato (Ipomoea batatas, which is hindering genetic research in this species. It is necessary to develop more molecular markers for potential use in sweetpotato genetic research. With the newly developed next generation sequencing technology, large amount of transcribed sequences of sweetpotato have been generated and are available for identifying SSR markers by data mining. Results In this study, we investigated 181,615 ESTs for the identification and development of SSR markers. In total, 8,294 SSRs were identified from 7,163 SSR-containing unique ESTs. On an average, one SSR was found per 7.1 kb of EST sequence with tri-nucleotide motifs (42.9% being the most abundant followed by di- (41.2%, tetra- (9.2%, penta- (3.7% and hexa-nucleotide (3.1% repeat types. The top five motifs included AG/CT (26.9%, AAG/CTT (13.5%, AT/TA (10.6%, CCG/CGG (5.8% and AAT/ATT (4.5%. After removing possible duplicate of published EST-SSRs of sweetpotato, a total of non-repeat 7,958 SSR motifs were identified. Based on these SSR-containing sequences, 1,060 pairs of high-quality SSR primers were designed and used for validation of the amplification and assessment of the polymorphism between two parents of one mapping population (E Shu 3 Hao and Guang 2k-30 and eight accessions of cultivated sweetpotatoes. The results showed that 816 primer pairs could yield reproducible and strong amplification products, of which 195 (23.9% and 342 (41.9% primer pairs exhibited polymorphism between E Shu 3 Hao and Guang 2k-30 and among the 8 cultivated sweetpotatoes, respectively. Conclusion This study gives an insight into the frequency, type and distribution of sweetpotato EST-SSRs and demonstrates successful development of EST-SSR markers in cultivated sweetpotato. These EST-SSR markers could enrich the current resource of molecular markers for the sweetpotato community and would

  2. Characterization and development of EST-derived SSR markers in cultivated sweetpotato (Ipomoea batatas)

    Science.gov (United States)

    2011-01-01

    Background Currently there exists a limited availability of genetic marker resources in sweetpotato (Ipomoea batatas), which is hindering genetic research in this species. It is necessary to develop more molecular markers for potential use in sweetpotato genetic research. With the newly developed next generation sequencing technology, large amount of transcribed sequences of sweetpotato have been generated and are available for identifying SSR markers by data mining. Results In this study, we investigated 181,615 ESTs for the identification and development of SSR markers. In total, 8,294 SSRs were identified from 7,163 SSR-containing unique ESTs. On an average, one SSR was found per 7.1 kb of EST sequence with tri-nucleotide motifs (42.9%) being the most abundant followed by di- (41.2%), tetra- (9.2%), penta- (3.7%) and hexa-nucleotide (3.1%) repeat types. The top five motifs included AG/CT (26.9%), AAG/CTT (13.5%), AT/TA (10.6%), CCG/CGG (5.8%) and AAT/ATT (4.5%). After removing possible duplicate of published EST-SSRs of sweetpotato, a total of non-repeat 7,958 SSR motifs were identified. Based on these SSR-containing sequences, 1,060 pairs of high-quality SSR primers were designed and used for validation of the amplification and assessment of the polymorphism between two parents of one mapping population (E Shu 3 Hao and Guang 2k-30) and eight accessions of cultivated sweetpotatoes. The results showed that 816 primer pairs could yield reproducible and strong amplification products, of which 195 (23.9%) and 342 (41.9%) primer pairs exhibited polymorphism between E Shu 3 Hao and Guang 2k-30 and among the 8 cultivated sweetpotatoes, respectively. Conclusion This study gives an insight into the frequency, type and distribution of sweetpotato EST-SSRs and demonstrates successful development of EST-SSR markers in cultivated sweetpotato. These EST-SSR markers could enrich the current resource of molecular markers for the sweetpotato community and would be useful for

  3. Validation of an Estonian version of the Parkinson's Disease Questionnaire (PDQ-39

    Directory of Open Access Journals (Sweden)

    Lai Taavi

    2008-03-01

    Full Text Available Abstract Introduction Diagnosis and management of Parkinson's disease (PD rely heavily on evaluation of clinical symptoms and patients' subjective perception of their condition. The purpose of this study was to evaluate the validity, acceptability, and reliability of the Estonian version of the 39-question Parkinson 's disease Questionnaire (PDQ-39. Methods Study subjects were approached during their regular clinic follow-up visits. 104 patients consented to the study and 81 completed questionnaires were used for subsequent testing of psychometric characteristics, validity and reliability. Results The content validity was assessed through qualitative content analysis during the pilot study. The patients indicated that the questions were relevant to measure the quality of life of people with PD. The analysis of means showed that the ceiling and floor effects of domain results were within the limits of 15% of Summary Index and of all domains except Stigma, Social Support and Communication where the ceiling effect was 16% to 24% of the responses. Convergent validity was interpreted through correlation between disease severity and PDQ-39 domains. There was a statistically significant difference between the domain scores in patients with mild versus moderate PD in domains of Mobility, ADL, and Communication but not for Stigma, Social Support and Cognition. The reliability was good, Cronbach alpha for all domains and summary index was over 0.8 and item-test correlations between domains and summary index ranged from 0.56 to 0.83. Conclusion The psychometric characteristics of an Estonian version of the PDQ-39 were satisfactory. The results of this study were comparable to the results of previous validation studies in other cultural settings in UK, USA, Canada, Spain and Italy. The Estonian version of the PDQ-39 is an acceptable, valid and reliable instrument for quality of life measurement in PD patients.

  4. Trends in smoking behaviour among Estonian physicians in 1982-2014.

    Science.gov (United States)

    Pärna, Kersti; Põld, Mariliis; Ringmets, Inge

    2017-07-25

    Smoking surveys among physicians have proved useful in highlighting the importance of physicians as healthy life style exemplars and role models in tobacco control and smoking cessation. The aim of this study was to give an overview of smoking behaviour among Estonian physicians from 1982 to 2014. Three cross-sectional postal surveys using a self-administered questionnaire were carried out among all practising physicians in Estonia. The number of physicians participating in this study was 3786 in 1982, 2735 in 2002, and 2902 in 2014. Data analysis involved calculating the age-standardized prevalences of smoking, prevalences of smoking by age group and mean age of smoking initiation. A non-parametric test for trend was used to assess significant changes in smoking over time. Age-standardized prevalence of current smoking among men was 39.7% in 1982, 20.9% in 2002, and 14.3% in 2014 and among women 12.2%, 8.0%, and 5.2%, respectively (p smoking among men and women was in age groups under 35 (from 55.2% to 16.7% and from 16.7% to 2.8%, respectively) and 35-44 (from 47.1% to 8.3% and from 19.5% to 5.1%, respectively) (p smoking initiation decreased from 20.4 to 19.3 among men and from 24.5 to 20.4 among women over the study period. In 1982-2014, smoking prevalence among Estonian physicians declined substantially. This may influence the willingness of society to recognize the health consequences of smoking which could give a support to the decline of the smoking epidemic in the country. Differences between smoking among male and female physicians persisted over the study period, but mean age of smoking initiation decreased. A further decline in smoking among Estonian physicians should be encouraged by special efforts targeted at physicians.

  5. Genetic diversity and DNA fingerprinting in jute (Corchorus spp. based on SSR markers

    Directory of Open Access Journals (Sweden)

    Liwu Zhang

    2015-10-01

    Full Text Available Genetic diversity analysis and DNA finger printing are very useful in breeding programs, seed conservation and management. Jute (Corchorus spp. is the second most important natural fiber crop after cotton. DNA fingerprinting studies in jute using SSR markers are limited. In this study, 58 jute accessions, including two control varieties (Huangma 179 and Kuanyechangguo from the official variety registry in China were evaluated with 28 pairs of SSR primers. A total of 184 polymorphic loci were identified. Each primer detected 3 to 15 polymorphic loci, with an average of 6.6. The 58 jute accessions were DNA-fingerprinted with 67 SSR markers from the 28 primer pairs. These markers differentiated the 58 jute accessions from one another, with CoSSR305-120 and CoSSR174-195 differentiating Huangma 179 and Kuanyechangguo, respectively. NTSYS-pc2.10 software was used to analyze the genetic diversity in the 58 jute accessions. Their genetic similarity coefficients ranged from 0.520 to 0.910 with an average of 0.749, indicating relatively great genetic diversity among them. The 58 jute accessions were divided into four groups with the coefficient 0.710 used as a value for classification, consistent with their species and pedigrees. All these results may be useful both for protection of intellectual property rights of jute accessions and for jute improvement.

  6. Investigation of SSR Characteristics of Hybrid Series Compensated Power System with SSSC

    Directory of Open Access Journals (Sweden)

    R. Thirumalaivasan

    2011-01-01

    Full Text Available The advent of series FACTS controllers, thyristor controlled series capacitor (TCSC and static synchronous Series Compensator (SSSC has made it possible not only for the fast control of power flow in a transmission line, but also for the mitigation of subsynchronous resonance (SSR in the presence of fixed series capacitors. SSSC is an emerging controller and this paper presents SSR characteristics of a series compensated system with SSSC. The study system is adapted from IEEE first benchmark model (FBM. The active series compensation is provided by a three-level twenty four-pulse SSSC. The modeling and control details of a three level voltage source converter-(VSC-based SSSC are discussed. The SSR characteristics of the combined system with constant reactive voltage control mode in SSSC has been investigated. It is shown that the constant reactive voltage control of SSSC has the effect of reducing the electrical resonance frequency, which detunes the SSR. The analysis of SSR with SSSC is carried out based on frequency domain method, eigenvalue analysis and transient simulation. While the eigenvalue and damping torque analysis are based on linearizing the D-Q model of SSSC, the transient simulation considers both D-Q and detailed three phase nonlinear system model using switching functions.

  7. Development and Characterization of 37 Novel EST-SSR Markers in Pisum sativum (Fabaceae

    Directory of Open Access Journals (Sweden)

    Xiaofeng Zhuang

    2013-01-01

    Full Text Available Premise of the study: Simple sequence repeat markers were developed based on expressed sequence tags (EST-SSR and screened for polymorphism among 23 Pisum sativum individuals to assist development and refinement of pea linkage maps. In particular, the SSR markers were developed to assist in mapping of white mold disease resistance quantitative trait loci. Methods and Results: Primer pairs were designed for 46 SSRs identified in EST contiguous sequences assembled from a 454 pyrosequenced transcriptome of the pea cultivar, ‘LIFTER’. Thirty-seven SSR markers amplified PCR products, of which 11 (30% SSR markers produced polymorphism in 23 individuals, including parents of recombinant inbred lines, with two to four alleles. The observed and expected heterozygosities ranged from 0 to 0.43 and from 0.31 to 0.83, respectively. Conclusions: These EST-SSR markers for pea will be useful for refinement of pea linkage maps, and will likely be useful for comparative mapping of pea and as tools for marker-based pea breeding.

  8. Development and characterization of microsatellite markers (SSR) in Sesamum (Sesamum indicum L.) species.

    Science.gov (United States)

    Spandana, B; Reddy, V Prathap; Prasanna, G John; Anuradha, G; Sivaramakrishnan, S

    2012-11-01

    Microsatellites, also known as simple sequence repeats (SSRs), are the class of repetitive DNA sequences present throughout the genome of many plant and animal species. Recent advances in molecular genetics had been the introduction of microsatellite markers to investigate the genetic structuring of natural plant populations. We have employed an enrichment strategy for microsatellite isolation by using multi-enzymes digestion, microsatellite oligoprobes, and streptavidin magnetic beads in Sesamum (Sesamum indicum L.). More than 200 SSR motifs were detected (SSR motifs ≥2 repeat units or 6 bp); 80 % of the clones contained SSR motifs. When regarding SSRs with four or more repeat units and a minimum length of 10 bp, 132 of them showed repeats. Eighteen SSR markers were initially characterized for optimum annealing temperature using a gradient PCR technique. Among the 18 SSR markers characterized, five were found to be polymorphic and used to analyze 60 Sesamum germplasm accessions. The maximum number of alleles detected was four with a single primer and the least number of two alleles with three primers with an average PIC value of 0.77. SSRs are a valuable tool for estimating genetic diversity and analyzing the evolutionary and historical development of cultivars at the genomic level in sesame breeding programs.

  9. Molecular evaluations of thirty one clones of poplar based on RAPD and SSR molecular markers

    Directory of Open Access Journals (Sweden)

    Singh M.K.

    2014-01-01

    Full Text Available Poplar is an important tree species valued all over the world for its wood importance. Despite limited knowledge of the levels of genetic diversity and relatedness, their cultivation as a source of plywood is widespread. In order to facilitate reasoned scientific decisions on its management and conservation and prepare for selective breeding programme, genetic analysis of 31 genotypes was performed using RAPD and SSR molecular markers. Twenty six RAPD primers and 14 SSR primers amplified a total of 236 and 85 scoreable bands of which 86.44% and 86.02% were polymorphic. The mean coefficient of gene differentiation (Gst was 0.388 and 0.341 indicating that 61.2% and 65.9% of the genetic variation resided within the populations. Analysis of molecular variance (AMOVA indicated that majority of genetic variation (94.6% using RAPD and 89% using SSR occurred among genotypes, while the variation between the three groups (categorized as tall, medium and small plants height was 5.4% (using RAPD and 11% (using SSR. The dendrogram obtained from NJ and STRUCTURE analysis revealed splitting of genotypes into four clusters with clear distinction between short, medium and tall height genotypes, indicated that genetic differentiations measure with respect to RAPD and SSR. However, both the markers were equally useful in providing some understanding about the genetic relationship of different genotypes of poplar that are important in the conservation and exploitation of poplar genetic resources.

  10. Genetic characterization of pea (Pisum sativum) germplasm from Turkey using morphological and SSR markers.

    Science.gov (United States)

    Sarikamiş, G; Yanmaz, R; Ermiş, S; Bakir, M; Yüksel, C

    2010-03-30

    The need for the conservation of plant genetic resources has been widely accepted. Germplasm characterization and evaluation yield information for more efficient utilization of these valuable resources. The aim of the present study was to characterize the pea germplasm conserved at the Aegean Agricultural Research Institute of Turkey using morphological and simple sequence repeat (SSR)-based molecular approaches. Genetic characterization of 30 pea genotypes collected from different regions of Turkey and 10 commercial pea cultivars was performed using the criteria of the International Union for the Protection of New Varieties of Plants (UPOV) (TG 7/9 Pisum sativum), and with 10 SSR markers. We originally tested 15 SSR markers; 10 of these markers were selected on the basis of high polymorphism information content in the molecular assays. Sixty-one alleles were detected at the 10 loci. The number of alleles per SSR locus ranged from 3 (PVSBE2) to 12 (AB53), with a mean of 6.1 alleles. The most informative loci were AB53 (12 alleles), AA355 (9 alleles), AD270 (8 alleles), A9 (7 alleles), AD61 (7 alleles), and AB25 (6 alleles). The UPGMA dendrogram defined by SSR markers revealed genetic relatedness of the pea genotypes. These findings can be used to guide future breeding studies and germplasm management of these pea genotypes.

  11. SSR-based genetic linkage map of Cucurbita moschata and its synteny with Cucurbita pepo.

    Science.gov (United States)

    Gong, L; Pachner, M; Kalai, K; Lelley, T

    2008-11-01

    The first SSR-based genetic linkage map of Cucurbita moschata was created by integrating the maps of two F2 populations with one common parent developed from the crosses Waltham Butternut (WB) x Nigerian Local (NL) and ZHOU (a hull-less type) x WB. The integrated C. moschata map comprises 205 SSR markers and two morphological traits (Gr and n). The map is composed of 27 linkage groups with a marker density of 7 cM. Comparing the C. moschata map with the published Cucurbita pepo map, we found a high level of macrosynteny. Seventy-two of 76 common SSR markers between C. moschata and C. pepo were located in homologous linkage groups. These markers in general have conserved orders and similar genetic distances; they represent orthologous loci. A reference map based on these SSRs was obtained. No major chromosomal rearrangement between the two species could be detected at present, although four SSR markers were mapped in nonhomologous linkage groups. The comparative alignment of SSR markers did not provide any indication of a possible ancient polyploid origin of the species. The comparative mapping of C. moschata and C. pepo reported here will be useful for further studies on Cucurbit evolution, gene isolation, and breeding work.

  12. Identification of molecular performance from oil palm clones based on SSR markers

    Science.gov (United States)

    Putri, Lollie Agustina P.; Basyuni, M.; Bayu, Eva S.; Arvita, D.; Arifiyanto, D.; Syahputra, I.

    2018-03-01

    In Indonesia, the oil palms are an important economic crop, producing food and raw materials for the food, confectionary, cosmetics and oleo-chemical industrial demands of oil palm products. Clonal oil palm offers the potential for greater productivity because it is possible to establish uniform tree stands comprising identical copies (clones) of a limited number of highly productive oil palms. Unfortunately, tissue culture sometimes accentuates the expression of detects in oil palm, particularly when embryogenesis is induced in particullar callus for prolonged periods. This research is conducted by taking individual tree sample of clone germplasm two years old. The purpose of this research is to molecular performance analysis of some oil palm clones based on SSR markers. A total of 30 trees oil palm clones were used for analysis. In this experiment, the DNA profile diversity was assessed using five loci of oil palm’s specific SSR markers. The results of the experiment indicated out of 3 SSR markers (FR-0779, FR-3663 and FR-0782) showed monomorphic of PCR product and 2 SSR markers (FR-0783 and FR- 3745) showed polymorphic of PCR product. There are 10 total number of PCR product. These preliminary results demonstrated SSR marker can be used to evaluate genetic relatedness among trees of oil palm clones.

  13. ALARM, a life saving training program for inpatient mental health care staff. Tallinn, Estland (27-30 augustus 2014) : Oral presentation European Symposium on Suicide and Suicidal Behaviour

    NARCIS (Netherlands)

    Nienke Kool

    2014-01-01

    Oral presentation European Symposium on Suicide and Suicidal Behaviour. Titel: ALARM, a life saving training program for inpatient mental health care staff. Tallinn, Estland (27-30 augustus 2014) Introduction Despite precautions, suicide does happen. Sometimes patients are found while attempting

  14. Retsensioon : Riigikogu VII, VIII ja IX koosseis. Statistikat ja kommentaare. Peatoimetaja Aaro Mõttus. Tallinn : Riigikogu Kantselei, Eesti Rahvusraamatukogu, 2004. 326 lk. / Mart Nutt

    Index Scriptorium Estoniae

    Nutt, Mart, 1962-

    2004-01-01

    Arvustus: Riigikogu VII, VIII ja IX koosseis. Statistikat ja kommentaare / peatoim. Aaro Mõttus. Tallinn : Riigikogu Kantselei, Eesti Rahvusraamatukogu, 2004. - Lisatud: Ott Heinapuu tutvustav sõnum retsenseeritud väljaande kohta lk. 193. - Vt. ka: http://www.riigikogu.ee/rito/index.php?id=11357

  15. Estonian experience in establishing the national radiation protection infrastructure in the newly independent State

    International Nuclear Information System (INIS)

    Kalam, J.

    2001-01-01

    The Estonian Radiation Protection Centre (ERPC) was established on 4 January 1996 as the regulatory authority for radiation protection and safety of radiation sources. The report explains the ERPC's structure and its main functions and activities, and provides information on the regulations that have been approved or are planned to be adopted. Reference is made to radiological emergency preparedness and, in particular, to the status of development of the system of regulatory control by authorization and inspection of radiation practices in the country. (author)

  16. Variation in tryptophan hydroxylase-2 gene is not associated to male completed suicide in Estonian population.

    Science.gov (United States)

    Must, Anne; Tasa, Gunnar; Lang, Aavo; Vasar, Eero; Kõks, Sulev; Maron, Eduard; Väli, Marika

    2009-04-03

    Dysfunction of the central serotonergic system has been related to a spectrum of psychiatric disorders, including suicidal behavior. Tryptophan hydroxylase isoform 2 (TPH2) is the rate-limiting enzyme in the biosynthetic pathway of serotonin, being expressed in serotonergic neurons of raphe nuclei. We investigated genetic variation in TPH2 gene in two samples of male subjects: 288 suicide completers and 327 volunteers, in order to reveal any associations between 14 single nucleotide polymorphisms and completed suicide. No associations were revealed neither on allelic nor haplotype level. Our finding does not support the hypothesis of TPH2 being a susceptibility factor for completed suicide in males of Estonian origin.

  17. Development of unigene-derived SSR markers in cowpea (Vigna unguiculata) and their transferability to other Vigna species.

    Science.gov (United States)

    Gupta, S K; Gopalakrishna, T

    2010-07-01

    Unigene sequences available in public databases provide a cost-effective and valuable source for the development of molecular markers. In this study, the identification and development of unigene-based SSR markers in cowpea (Vigna unguiculata (L.) Walp.) is presented. A total of 1071 SSRs were identified in 15 740 cowpea unigene sequences downloaded from the National Center for Biotechnology Information. The most frequent SSR motifs present in the unigenes were trinucleotides (59.7%), followed by dinucleotides (34.8%), pentanucleotides (4%), and tetranucleotides (1.5%). The copy number varied from 6 to 33 for dinucleotide, 5 to 29 for trinucleotide, 5 to 7 for tetranucleotide, and 4 to 6 for pentanucleotide repeats. Primer pairs were successfully designed for 803 SSR motifs and 102 SSR markers were finally characterized and validated. Putative function was assigned to 64.7% of the unigene SSR markers based on significant homology to reported proteins. About 31.7% of the SSRs were present in coding sequences and 68.3% in untranslated regions of the genes. About 87% of the SSRs located in the coding sequences were trinucleotide repeats. Allelic variation at 32 SSR loci produced 98 alleles in 20 cowpea genotypes. The polymorphic information content for the SSR markers varied from 0.10 to 0.83 with an average of 0.53. These unigene SSR markers showed a high rate of transferability (88%) across other Vigna species, thereby expanding their utility. Alignment of unigene sequences with soybean genomic sequences revealed the presence of introns in amplified products of some of the SSR markers. This study presents the distribution of SSRs in the expressed portion of the cowpea genome and is the first report of the development of functional unigene-based SSR markers in cowpea. These SSR markers would play an important role in molecular mapping, comparative genomics, and marker-assisted selection strategies in cowpea and other Vigna species.

  18. Characterization of SSR genomic abundance and identification of SSR markers for population genetics in Chinese jujube (Ziziphus jujuba Mill.

    Directory of Open Access Journals (Sweden)

    Peng-cheng Fu

    2016-02-01

    Full Text Available Chinese jujube (Ziziphus jujuba Mill. [Rhamnaceae], native to China, is a major dried fruit crop in Asia. Although many simple sequence repeat (SSR markers are available for phylogenetic analysis of jujube cultivars, few of these are validated on the level of jujube populations. In this study, we first examined the abundance of jujube SSRs with repeated unit lengths of 1–6 base pairs, and compared their distribution with those in Arabidopsis thaliana. We identified 280,596 SSRs in the assembled genome of jujube. The density of SSRs in jujube was 872.60 loci/Mb, which was much higher than in A. thaliana (221.78 loci/Mb. (A+ T-rich repeats were dominant in the jujube genome. We then randomly selected 100 SSRs in the jujube genome with long repeats and used them to successfully design 70 primer pairs. After screening using a series of criteria, a set of 20 fluorescently labeled primer pairs was further selected and screened for polymorphisms among three jujube populations. The average number of alleles per locus was 12.8. Among the three populations, mean observed and expected heterozygosities ranged from 0.858 to 0.967 and 0.578 to 0.844, respectively. After testing in three populations, all SSRs loci were in Hardy-Weinberg equilibrium (HWE in at least one population. Finally, removing high null allele frequency loci and linked loci, a set of 17 unlinked loci was in HWE. These markers will facilitate the study of jujube genetic structure and help elucidate the evolutionary history of this important fruit crop.

  19. Three Decades of Sustained Silent Reading: A Meta-Analytic Review of the Effects of SSR on Attitude toward Reading.

    Science.gov (United States)

    Yoon, Jun-Chae

    2002-01-01

    Investigates the overall effect of Sustained Silent Reading (SSR) on attitude toward reading and identifies the moderator variables of SSR on it. Suggests that providing a fixed period of time for students to read materials of their own choosing either for pleasure or for information facilitates their attitude toward reading. Supports the…

  20. WGSSAT: A High-Throughput Computational Pipeline for Mining and Annotation of SSR Markers From Whole Genomes.

    Science.gov (United States)

    Pandey, Manmohan; Kumar, Ravindra; Srivastava, Prachi; Agarwal, Suyash; Srivastava, Shreya; Nagpure, Naresh S; Jena, Joy K; Kushwaha, Basdeo

    2018-03-16

    Mining and characterization of Simple Sequence Repeat (SSR) markers from whole genomes provide valuable information about biological significance of SSR distribution and also facilitate development of markers for genetic analysis. Whole genome sequencing (WGS)-SSR Annotation Tool (WGSSAT) is a graphical user interface pipeline developed using Java Netbeans and Perl scripts which facilitates in simplifying the process of SSR mining and characterization. WGSSAT takes input in FASTA format and automates the prediction of genes, noncoding RNA (ncRNA), core genes, repeats and SSRs from whole genomes followed by mapping of the predicted SSRs onto a genome (classified according to genes, ncRNA, repeats, exonic, intronic, and core gene region) along with primer identification and mining of cross-species markers. The program also generates a detailed statistical report along with visualization of mapped SSRs, genes, core genes, and RNAs. The features of WGSSAT were demonstrated using Takifugu rubripes data. This yielded a total of 139 057 SSR, out of which 113 703 SSR primer pairs were uniquely amplified in silico onto a T. rubripes (fugu) genome. Out of 113 703 mined SSRs, 81 463 were from coding region (including 4286 exonic and 77 177 intronic), 7 from RNA, 267 from core genes of fugu, whereas 105 641 SSR and 601 SSR primer pairs were uniquely mapped onto the medaka genome. WGSSAT is tested under Ubuntu Linux. The source code, documentation, user manual, example dataset and scripts are available online at https://sourceforge.net/projects/wgssat-nbfgr.

  1. Genetic variation and DNA fingerprinting of durian types in Malaysia using simple sequence repeat (SSR) markers.

    Science.gov (United States)

    Siew, Ging Yang; Ng, Wei Lun; Tan, Sheau Wei; Alitheen, Noorjahan Banu; Tan, Soon Guan; Yeap, Swee Keong

    2018-01-01

    Durian ( Durio zibethinus ) is one of the most popular tropical fruits in Asia. To date, 126 durian types have been registered with the Department of Agriculture in Malaysia based on phenotypic characteristics. Classification based on morphology is convenient, easy, and fast but it suffers from phenotypic plasticity as a direct result of environmental factors and age. To overcome the limitation of morphological classification, there is a need to carry out genetic characterization of the various durian types. Such data is important for the evaluation and management of durian genetic resources in producing countries. In this study, simple sequence repeat (SSR) markers were used to study the genetic variation in 27 durian types from the germplasm collection of Universiti Putra Malaysia. Based on DNA sequences deposited in Genbank, seven pairs of primers were successfully designed to amplify SSR regions in the durian DNA samples. High levels of variation among the 27 durian types were observed (expected heterozygosity, H E  = 0.35). The DNA fingerprinting power of SSR markers revealed by the combined probability of identity (PI) of all loci was 2.3×10 -3 . Unique DNA fingerprints were generated for 21 out of 27 durian types using five polymorphic SSR markers (the other two SSR markers were monomorphic). We further tested the utility of these markers by evaluating the clonal status of shared durian types from different germplasm collection sites, and found that some were not clones. The findings in this preliminary study not only shows the feasibility of using SSR markers for DNA fingerprinting of durian types, but also challenges the current classification of durian types, e.g., on whether the different types should be called "clones", "varieties", or "cultivars". Such matters have a direct impact on the regulation and management of durian genetic resources in the region.

  2. Development of a set of SSR markers for genetic polymorphism detection and interspecific hybrid jute breeding

    Directory of Open Access Journals (Sweden)

    Dipnarayan Saha

    2017-10-01

    Full Text Available Corchorus capsularis (white jute and C. olitorius (dark jute are the two principal cultivated species of jute that produce natural bast fiber of commercial importance. We have identified 4509 simple sequence repeat (SSR loci from 34,163 unigene sequences of C. capsularis to develop a non-redundant set of 2079 flanking primer pairs. Among the SSRs, trinucleotide repeats were most frequent (60% followed by dinucleotide repeats (37.6%. Annotation of the SSR-containing unigenes revealed their putative functions in various biological and molecular processes, including responses to biotic and abiotic signals. Eighteen expressed gene-derived SSR (eSSR markers were successfully mapped to the existing single-nucleotide polymorphism (SNP linkage map of jute, providing additional anchor points. Amplification of 72% of the 74 randomly selected primer pairs was successful in a panel of 24 jute accessions, comprising five and twelve accessions of C. capsularis and C. olitorius, respectively, and seven wild jute species. Forty-three primer pairs produced an average of 2.7 alleles and 58.1% polymorphism in a panel of 24 jute accessions. The mean PIC value was 0.34 but some markers showed PIC values higher than 0.5, suggesting that these markers can efficiently measure genetic diversity and serve for mapping of quantitative trait loci (QTLs in jute. A primer polymorphism survey with parents of a wide-hybridized population between a cultivated jute and its wild relative revealed their efficacy for interspecific hybrid identification. For ready accessibility of jute eSSR primers, we compiled all information in a user-friendly web database, JuteMarkerdb (http://jutemarkerdb.icar.gov.in/ for the first time in jute. This eSSR resource in jute is expected to be of use in characterization of germplasm, interspecific hybrid and variety identification, and marker-assisted breeding of superior-quality jute.

  3. Comparative assessment of genetic diversity in Sesamum indicum L. using RAPD and SSR markers.

    Science.gov (United States)

    Dar, Aejaz Ahmad; Mudigunda, Sushma; Mittal, Pramod Kumar; Arumugam, Neelakantan

    2017-05-01

    Sesame (Sesamum indicum L.) is an ancient oilseed crop known for its nutty seeds and high-quality edible oil. It is an unexplored crop with a great economic potential. The present study deals with assessment of genetic diversity in the crop. Twenty two RAPD and 18 SSR primers were used for analysis of the 47 different sesame accessions grown in different agroclimatic zones of India. A total of 256 bands were obtained with RAPD primers, of which 191 were polymorphic. SSR primers gave 64 DNA bands, of which all of were polymorphic. The Jaccard's similarity coefficient of RAPD, SSR, and pooled RAPD and SSR data ranged from 0.510 to 0.885, 0.167 to 0.867, and 0.505 to 0.853, respectively. Maximum polymorphic information content was reported with SSRs (0.194) compared to RAPDs (0.186). Higher marker index was observed with RAPDs (1.426) than with SSRs (0.621). Similarly, maximum resolving power was found with RAPD (4.012) primers than with SSRs (0.884). The RAPD primer RPI-B11 and SSR primer S16 were the most informative in terms of describing genetic variability among the varieties under study. At a molecular level, the seed coat colour was distinguishable by the presence and absence of a group of marker amplicon/s. White and brown seeded varieties clustered close to each other, while black seeded varieties remained distanced from the cluster. In the present study, we found higher variability in Sesamum indicum L. using RAPD and SSR markers and these could assist in DNA finger printing, conservation of germplasm, and crop improvement.

  4. External factors and the interference of L1 Estonian on L2 English pronunciation: An apparent-time study

    Directory of Open Access Journals (Sweden)

    Kristiina Ader

    2015-04-01

    Full Text Available This study focusses on external factors of second language learning (L2 and their effect on L2 sound production. The aim was to find out whether young adults whose first language (L1 is Estonian speak L2 English with less accent than older speakers of L1 Estonian. Prior studies have claimed that more exposure to the target language lessens the effect of L1 interference (internal factors (e.g. Piske et al. 2001, Muñoz, Llanes 2014. An apparent-time study was imple- mented in order to test whether a change in learner setting (i.e. limited exposure to the target language vs. abundant exposure shows any evidence which can be explained by such external factors. Data was elicited from the speech samples of 97 speakers of L1 Estonian and L1 interference effects were analysed sound by sound. The results show that the younger generation displays less L1 Estonian interference than the older generation. Both greater exposure to the target language and earlier age of L2 learning onset correlate with the ability to produce more sounds target-like.

  5. Exploring Constructivist Social Learning Practices in Aiding Russian-Speaking Teachers to Learn Estonian: An Action Research Approach

    Science.gov (United States)

    Kiilo, Tatjana; Kutsar, Dagmar

    2012-01-01

    Based on appreciative inquiry and threshold concepts from an intercultural learning perspective, the article makes insights into the constructivist social learning practice of Estonian language learning amongst Russian-speaking teachers in Estonia. The application of educational action research methodology, more specifically that of Bridget…

  6. Proficiency Assessment of Male Volleyball Teams of the 13-15-Year Age Group at Estonian Championships

    Science.gov (United States)

    Stamm, Meelis; Stamm, Raini; Koskel, Sade

    2008-01-01

    Study aim: Assessment of feasibility of using own computer software "Game" at competitions. Material and methods: The data were collected during Estonian championships in 2006 for male volleyball teams of the 13-15-years age group (n = 8). In all games, the performance of both teams was recorded in parallel with two computers. A total of…

  7. The Winding Road on the Media Landscape: The Establishment of Estonian (Television) Broadcasting between 1992 and 2016

    NARCIS (Netherlands)

    Jõesaar, Andres

    2017-01-01

    textabstractThis article aims to explore the ways in which Estonian broadcasting (with a focus on television) tackled the challenges of transforming from a monopolistic party propaganda machine into a modern dual media system in which public service broadcasting and newly created private enterprises

  8. Enhancing Teachers' Curriculum Ownership via Teacher Engagement in State-Based Curriculum-Making: The Estonian Case

    Science.gov (United States)

    Mikser, Rain; Kärner, Anita; Krull, Edgar

    2016-01-01

    Teachers' curriculum ownership is increasingly gaining attention in many countries. It is particularly important that under the conditions of centralized curriculum-making, teachers as final implementers of curricular ideas identify themselves with these ideas. This study investigates Estonian upper secondary school teachers' views on the impact…

  9. Tabud ja reeglid. Sissevaateid eesti laagriromaani / Taboos and Rules. Insights into Prison Camp Novels by Estonian Writers

    Directory of Open Access Journals (Sweden)

    Anneli Kõvamees

    2013-12-01

    Full Text Available The article concentrates on Estonian novels depicting Soviet prison camps in the 1940s and 1950s. The goal is to map themes, motifs and characteristics in such novels, concentrating on various taboos and rules in the prison camp environment. For a long time the Soviet prison camp theme was not publicly discussed in Estonia due to political reasons. Texts dealing with prison camps could appear in print only outside the Soviet Union; the way Estonians saw these historical events and hellish experiences were depicted mostly in exile novels. Most notable are the novels by Arved Viirlaid (b. 1922, e.g., Kes tappis Eerik Hormi? (Who Killed Eerik Horm? (1974, Surnud ei loe (The Dead do not Read (1975, Vaim ja ahelad (Mind and Chains (1961. Estonian prison camp novels can be seen as “the literature of testimony”, to use the term by Leona Toker. Dramatic historical events are written down to record the events and to show the inhumane nature of Soviet society. These records of the dramatic past follow certain patterns and create certain self- and hetero-images. A prison camp is a closed territory within a closed territory; prison camps can be seen as small models of Soviet society. Prison camp novels give a detailed view of the environment of the prison camp, its inhabitants and activities. Two central aspects are labour and food; the life of the prisoner whirls around these. The most important thing is to survive, which often leads to moral decline, e.g., stealing, cheating. However, there are lines Estonians do not cross, e.g., cannibalism or homosexual relationships with superiors. Estonians are always depicted as political prisoners (not common criminals and heterosexuals, while Russians are portrayed mainly as criminals and often also as homosexuals. Another important component of the image of the Estonians is their enterprising spirit and ability to manage even under very difficult conditions. Therefore, several oppositions can be identified, e

  10. BASIC COLOUR TERMS IN FIVE FINNOUGRIC LANGUAGES AND ESTONIAN SIGN LANGUAGE: A COMPARATIVE STUDY

    Directory of Open Access Journals (Sweden)

    Liivi Hollman

    2012-01-01

    Full Text Available In this paper we compare five Finno-Ugric languages – Estonian,Finnish, Hungarian, Udmurt and Komi-Zyrian – and the Estonian Sign Language (unclassified in different aspects: established basic colour terms, the proportion of basic colour terms and different colour terms in the collected word-corpora, the cognitive salience index values in the list task and the number of dominant colour tiles in the colour naming task. The data was collected, using the field method of Davies and Corbett, from all languages under consideration, providing a distinctive foundation for linguistic comparison. We argue that Finno-Ugric languages seem to possess relatively large colour vocabularies,especially due to their rich variety of word-formation types,e.g. the composition of compound words. All of the languages under consideration have developed to Stage VI or VII, possessing 7 to 11lexicalised basic colour terms. The cognitive salience index helps to distinguish primary and secondary basic colour terms, showing certain comprehensive patterns which are similar to Russian and English.

  11. Linking a population biobank with national health registries-the estonian experience.

    Science.gov (United States)

    Leitsalu, Liis; Alavere, Helene; Tammesoo, Mari-Liis; Leego, Erkki; Metspalu, Andres

    2015-04-16

    The Estonian population-based biobank, with 52,000 participants' genetic and health data, is the largest epidemiological cohort in the Baltic region. Participants were recruited through a network of medical professionals throughout Estonia (population 1.34 million). Unique legislation as well as a broad consent form give the Estonian Genome Center, a research institute of the University of Tartu, permission to re-contact participants and to retrieve participants' data from national registries and databases. In addition to two re-contacting projects to update the health data of participants, extensive clinical characterizations have been retrieved from national registries and hospital databases regularly since 2010. Acquiring data from electronic health records and registries has provided a means to update and enhance the database of the Genome Center in a timely manner and at low cost. The resulting database allows a wide spectrum of genomic and epidemiological research to be conducted with the aim of benefitting public health. Future plans include linking the genome center database with the national health information system through X-road and exchanging data in real time, as well as using the genetic data and the technical infrastructure available for piloting personalized medicine in Estonia.

  12. Linking a Population Biobank with National Health Registries—The Estonian Experience

    Science.gov (United States)

    Leitsalu, Liis; Alavere, Helene; Tammesoo, Mari-Liis; Leego, Erkki; Metspalu, Andres

    2015-01-01

    The Estonian population-based biobank, with 52,000 participants’ genetic and health data, is the largest epidemiological cohort in the Baltic region. Participants were recruited through a network of medical professionals throughout Estonia (population 1.34 million). Unique legislation as well as a broad consent form give the Estonian Genome Center, a research institute of the University of Tartu, permission to re-contact participants and to retrieve participants’ data from national registries and databases. In addition to two re-contacting projects to update the health data of participants, extensive clinical characterizations have been retrieved from national registries and hospital databases regularly since 2010. Acquiring data from electronic health records and registries has provided a means to update and enhance the database of the Genome Center in a timely manner and at low cost. The resulting database allows a wide spectrum of genomic and epidemiological research to be conducted with the aim of benefitting public health. Future plans include linking the genome center database with the national health information system through X-road and exchanging data in real time, as well as using the genetic data and the technical infrastructure available for piloting personalized medicine in Estonia. PMID:25894366

  13. Linking a Population Biobank with National Health Registries—The Estonian Experience

    Directory of Open Access Journals (Sweden)

    Liis Leitsalu

    2015-04-01

    Full Text Available The Estonian population-based biobank, with 52,000 participants’ genetic and health data, is the largest epidemiological cohort in the Baltic region. Participants were recruited through a network of medical professionals throughout Estonia (population 1.34 million. Unique legislation as well as a broad consent form give the Estonian Genome Center, a research institute of the University of Tartu, permission to re-contact participants and to retrieve participants’ data from national registries and databases. In addition to two re-contacting projects to update the health data of participants, extensive clinical characterizations have been retrieved from national registries and hospital databases regularly since 2010. Acquiring data from electronic health records and registries has provided a means to update and enhance the database of the Genome Center in a timely manner and at low cost. The resulting database allows a wide spectrum of genomic and epidemiological research to be conducted with the aim of benefitting public health. Future plans include linking the genome center database with the national health information system through X-road and exchanging data in real time, as well as using the genetic data and the technical infrastructure available for piloting personalized medicine in Estonia.

  14. Behind the Curtains of e-State: Determinants of Online Sexual Harassment among Estonian Children

    Directory of Open Access Journals (Sweden)

    Veronika Kalmus

    2012-11-01

    Full Text Available This article analyses the risk of receiving online sexual messages and experiencing harm among Estonian children. In particular, the paper examines the association between receiving sexual messages and behavioural, psychological and demographic characteristics, and the social mediation of children’s Internet use. Estonian data from EU Kids Online survey are used, involving 780 children aged 11-16. Results demonstrate that 19% of children who use the Internet have received online sexual messages, and 6% have felt disturbed. The probability of receiving sexual messages online is higher for children with risky online and offline behaviour and psychological difficulties. Perceiving online messages as sexually harassing is higher among children with excessive Internet use, lower levels of parental monitoring and higher levels of peer mediation of Internet use. The risk of exposure to harassing sexual messages also differs by age and, more notably, by the minority status. Mediation by parents and teachers plays an insignificant role in reducing teenagers’ risks of receiving sexual messages.

  15. Radon in Estonian buildings. Establishment of a measurement system and obtained results

    International Nuclear Information System (INIS)

    Pahapill, L.; Rulkov, A.; Swedjemark, G.A.

    1996-12-01

    One purpose of this project was the establishment of a radon monitoring programme inside the state environmental monitoring programme. Another purpose was to investigate regions, expected to have high radon levels indoors. A new method for the long-term measurement of indoor radon was established and the staff for these measurements was trained. The results of the measurement can be used by Estonian decision-makers to work out rules and standards. There is no legislative act in the field of radiation in Estonian at this time. To summarize the results of the measurements we can say that indoor radon concentrations vary by region. The radon investigations must be continued to identify the risk areas and types of housing construction. The results of the state radon monitoring are provided to the municipalities, who advice the owners of planned new houses to select the right construction for the house. A new project will follow with an investigation of radon in randomly selected dwellings, training and equipment for radon measurement in soil, and general advice with regard to radon, as well as assistance in preparing information about radon. 7 refs, 5 figs

  16. Mental health and alcohol problems among Estonian cleanup workers 24 years after the Chernobyl accident.

    Science.gov (United States)

    Laidra, Kaia; Rahu, Kaja; Tekkel, Mare; Aluoja, Anu; Leinsalu, Mall

    2015-11-01

    To study the long-term mental health consequences of the 1986 Chernobyl nuclear accident among cleanup workers from Estonia. In 2010, 614 Estonian Chernobyl cleanup workers and 706 geographically and age-matched population-based controls completed a mail survey that included self-rated health, the Posttraumatic Stress Disorder Checklist (PCL), alcohol symptoms (AUDIT), and scales measuring depressive, anxiety, agoraphobia, fatigue, insomnia, and somatization symptoms. Respondents were dichotomized into high (top quartile) and low symptom groups on each measure. Logistic regression analysis detected significant differences between cleanup workers and controls on all measures even after adjustment for ethnicity, education, marital status, and employment status. The strongest difference was found for somatization, with cleanup workers being three times more likely than controls to score in the top quartile (OR = 3.28, 95% CI 2.39-4.52), whereas for alcohol problems the difference was half as large (OR = 1.52, 95% CI 1.16-1.99). Among cleanup workers, arrival at Chernobyl in 1986 (vs. later) was associated with sleep problems, somatization, and symptoms of agoraphobia. The toll of cleanup work was evident 24 years after the Chernobyl accident among Estonian cleanup workers indicating the need for focused mental health interventions.

  17. Groundwater flow model of the Estonian oil shale mining area towards to innovative system

    Energy Technology Data Exchange (ETDEWEB)

    Lind, H. [Tallinn Univ. of Technology (Estonia). Dept. of Mining

    2010-07-01

    Changes in the Estonian groundwater regime are anticipated as oil shale deposits are mined. This paper described a dynamic groundwater flow model used to develop a 3-D groundwater elevation map of the Estonian oil shale mining area. The model was used to provide preliminary estimations of water inflow into the working underground mine areas. The model included 9 closed underground mines, 5 active mine sites, and 2 small open-cast sites. The closed mine sites were filled with water flowing in from the working mine sites. New mines and dewatering programs are planned for the future. A database from observation wells installed within the Keila-Kukruse aquifer was used to extract outputs and determine time steps. The model included 35 pumping stations from the active mine sites. The hydraulic properties for each model layer were defined in 4 model zones. Results of the model showed higher water in-flows from the closed underground sites than earlier predictions had anticipated. 9 refs., 1 tab., 4 figs.

  18. THE INFLUENCE OF SOCIAL STATUS AND ETHNICITY ON DIET IN MEDIAEVAL TALLINN AS SEEN THROUGH STABLE ISOTOPE ANALYSIS

    DEFF Research Database (Denmark)

    Lightfoot, Emma; Naum, Magdalena; Kadakas, Villu

    2016-01-01

    , for comparative purposes. The individuals were investigated using carbon, nitrogen and oxygen stable isotope analysis. The results indicate that while all analysed individuals consumed diets based on C3 plants and animals consuming C3 plants, some individuals also consumed small amounts of C4 plants (likely......Food encodes social and cultural values and has an important role to play in defining identities. In mixed populations, diet can be used to distinguish between ‘us’ and ‘them’. This study investigates the extent to which the inhabitants of mediaeval Tallinn, an important trading centre, used food...... millet) or seafood. C 4 plants were consumed by the urban residents buried at the Church of the Holy Spirit and one or two individuals from Sulevimägi. It is thought that millet was an imported food consumed by the middle class. Marine foods were consumed by the individuals at St Catherine’s Church...

  19. Vene kirjandus venestusaja eesti koolides. Russian Literature in the Estonian Schools of the Russification Era

    Directory of Open Access Journals (Sweden)

    Ülle Pärli

    2012-04-01

    Full Text Available This article aims to give an overview of literary instruction in schools of the russification era at the beginning of the 20th century in Estonia; this was likewise the curriculum of literary study offered to the generation of educated youth which included the Young Estonians. Based on official documents, archival materials, and memoirs, and through analyses of anthologies, literary histories, and teaching methods in use at the time, we attempt to reconstruct the outlines of literary reading and requirements for students in various types of schools. When, as a result of school reform, Russian became the language of instruction; lessons in Russian became central in the curriculum, alongside the word of God; selections from Russian literature were read in the original language. In the lower grades, teaching was by the so-called ”natural method”, intended to guarantee swift achievement of fluency in ”living Russian language”; this was later replaced by systematic textual analysis, which distinguished between belles lettres and other types of texts. However, the study of literature was always subordinated to the goals of language instruction. In institutions of secondary education, study of Russian literature was separate from language instruction. Indeed, Russian literature was the only literature systematically studied in high schools (though one must keep in mind that not all schools completely followed the official program. Private schools were especially noticeable for their greater freedom, though all of them had to take general curriculum directives into account. Reading of literary texts connected with other languages thus had to remain almost purely illustrative. In view of the above, in the upper grades of elementary school and high school, students obtained a thorough introduction to the Russian classics. According to the official school curriculum, Russian literary history ended with Nikolai Gogol. Attempts were made to ignore

  20. XML Genetic Structure of SSR1 & SSR2 loci from Iranian Mycobacterium Avium Subspecies Paratuberculosis Isolates by a Short Sequence Repeat Analysis Approach

    OpenAIRE

    Aida Chalesh (MSc); Keyvan Tadayon ( PhD

    2016-01-01

    Background and Objective: Paratuberculosis has been repeatedly reported from Iranian ruminant herds. The extrem fastidious nature of Mycobacterium avium subspecies paratuberculsos hinders genomic diversity studies of the pathogen. Short Sequence Repeat analysis is one of the genome-based approches recently developed to overcome this difficulty. In this study we describe the application of SSR genotyping on three Iranian MAP type strains plus the III & V vaccinal strain. Methods: A...

  1. Characterization of variable EST SSR markers for Norway spruce (Picea abies L.

    Directory of Open Access Journals (Sweden)

    Spiess Nadine

    2011-10-01

    Full Text Available Abstract Background Norway spruce is widely distributed across Europe and the predominant tree of the Alpine region. Fast growth and the fact that timber can be harvested cost-effectively in relatively young populations define its status as one of the economically most important tree species of Northern Europe. In this study, EST derived simple sequence repeat (SSR markers were developed for the assessment of putative functional diversity in Austrian Norway spruce stands. Results SSR sequences were identified by analyzing 14,022 publicly available EST sequences. Tri-nucleotide repeat motifs were most abundant in the data set followed by penta- and hexa-nucleotide repeats. Specific primer pairs were designed for sixty loci. Among these, 27 displayed polymorphism in a testing population of 16 P. abies individuals sampled across Austria and in an additional screening population of 96 P. abies individuals from two geographically distinct Austrian populations. Allele numbers per locus ranged from two to 17 with observed heterozygosity ranging from 0.075 to 0.99. Conclusions We have characterized variable EST SSR markers for Norway spruce detected in expressed genes. Due to their moderate to high degree of variability in the two tested screening populations, these newly developed SSR markers are well suited for the analysis of stress related functional variation present in Norway spruce populations.

  2. AFLP/SSR mapping of resistance genes to Alectra vogelii in cowpea ...

    African Journals Online (AJOL)

    user

    ., 1997) to total crop loss of the highly susceptible cultivar Black- eye (Riches, 1990). A. vogelii can produce as ... resistance gene Rac1 (Myers et al., 1996). Ouédraogo et al., (2001) ... RAPD (Garcia-Mas et al., 2000). The SSR technique is ...

  3. Analysis of esterase isozyme and SSR for mutagenic progenies induced by space mutation in mustard

    International Nuclear Information System (INIS)

    Shen Jinjuan; Liu Yihua; Zhang Zhaorong; Ran Guangkui; Zhao Shouzhong; Xiao Li

    2012-01-01

    Seeds of five mustard (Brassica juncea Coss) varieties were carried into outer space by 'Shijian No.8' satellite. After five years' consecutive planting and selection, ten relatively stable mutant lines were obtained, which had significant variation in agronomic and economic characters. The mutant lines and their original varieties without space mutation treatment as control were studied by esterase isozyme and SSR analyses. Electrophoresis analysis of esterase isozymes indicated that there were differences between mutant lines and their controls in enzyme types and enzyme activity. Different mustard varieties had different enzymographs, and so did the mutants induced by space mutation, which shows different sensitivity among different mustard varieties. The SSR analysis showed that large differences were found in the SSR loci between mutant lines and their original variety, the variation frequency was between 9.52% and 57.14% with an average frequency of 26.19% for all the mutant lines. Among the mutant SSR loci, about 56.36% showed changes in band number and 43.64% in molecular weight. These results indicated that the ten mutant lines had large genetic difference in phenotype, genomic sequence and gene expression, and the outer space mutation would be an effective method to develop new mustard germplasm and variety. (authors)

  4. Spanish version of the Sleep Self-Report (SSR): factorial structure and psychometric properties.

    Science.gov (United States)

    Orgilés, M; Owens, J; Espada, J P; Piqueras, J A; Carballo, J L

    2013-03-01

    The Sleep Self-Report (SSR) is a questionnaire initially created for use with a sample from the USA to assess sleep patterns and problems in school-aged children. The objective of this study was to validate the SSR among a Spanish sample. Participants were 1228 Spanish children from 8 to 12 years of age who completed the questionnaires at school anonymously. Internal consistency was good (ω = 0.85). Convergent validity with anxiety (r = 0.54) and perceived welfare (r = -0.53) measures, and divergent validity with a measure of academic performance and positive influence of peers (r = -0.22) were acceptable. Exploratory analysis suggested a factorial structure composed by four subscales: sleep quality, sleep anxiety, bedtime refusal and sleep routines. Confirmatory analysis indicated a good fit for the model (RMSEA = 0.04; GFI = 0.95; AGFI = 0.93; χ(2)/gl = 2.48). The SSR has demonstrated to have good psychometric properties in the Spanish-speaking sample of children. The factorial structure supported by exploratory and confirmatory analysis examines the most relevant areas of sleep in children. The satisfactory psychometric properties support the use of the Spanish version of the SSR by researchers and clinicians. © 2012 Blackwell Publishing Ltd.

  5. Development of a core set of SSR markers for the characterization of Gossypium germplasm

    Science.gov (United States)

    Molecular markers such as simple sequence repeats (SSR) are a useful tool for characterizing genetic diversity of Gossypium germplasm collections. Genetic profiles by DNA fingerprinting of cotton accessions can only be compared among different collections if a common set of molecular markers are us...

  6. Development of Genome-Wide SSR Markers from Angelica gigas Nakai Using Next Generation Sequencing

    Directory of Open Access Journals (Sweden)

    Jinsu Gil

    2017-09-01

    Full Text Available Angelica gigas Nakai is an important medicinal herb, widely utilized in Asian countries especially in Korea, Japan, and China. Although it is a vital medicinal herb, the lack of sequencing data and efficient molecular markers has limited the application of a genetic approach for horticultural improvements. Simple sequence repeats (SSRs are universally accepted molecular markers for population structure study. In this study, we found over 130,000 SSRs, ranging from di- to deca-nucleotide motifs, using the genome sequence of Manchu variety (MV of A. gigas, derived from next generation sequencing (NGS. From the putative SSR regions identified, a total of 16,496 primer sets were successfully designed. Among them, we selected 848 SSR markers that showed polymorphism from in silico analysis and contained tri- to hexa-nucleotide motifs. We tested 36 SSR primer sets for polymorphism in 16 A. gigas accessions. The average polymorphism information content (PIC was 0.69; the average observed heterozygosity (HO values, and the expected heterozygosity (HE values were 0.53 and 0.73, respectively. These newly developed SSR markers would be useful tools for molecular genetics, genotype identification, genetic mapping, molecular breeding, and studying species relationships of the Angelica genus.

  7. Development of Novel Polymorphic EST-SSR Markers in Bailinggu (Pleurotus tuoliensis for Crossbreeding

    Directory of Open Access Journals (Sweden)

    Yueting Dai

    2017-11-01

    Full Text Available Identification of monokaryons and their mating types and discrimination of hybrid offspring are key steps for the crossbreeding of Pleurotus tuoliensis (Bailinggu. However, conventional crossbreeding methods are troublesome and time consuming. Using RNA-seq technology, we developed new expressed sequence tag-simple sequence repeat (EST-SSR markers for Bailinggu to easily and rapidly identify monokaryons and their mating types, genetic diversity and hybrid offspring. We identified 1110 potential EST-based SSR loci from a newly-sequenced Bailinggu transcriptome and then randomly selected 100 EST-SSRs for further validation. Results showed that 39, 43 and 34 novel EST-SSR markers successfully identified monokaryons from their parent dikaryons, differentiated two different mating types and discriminated F1 and F2 hybrid offspring, respectively. Furthermore, a total of 86 alleles were detected in 37 monokaryons using 18 highly informative EST-SSRs. The observed number of alleles per locus ranged from three to seven. Cluster analysis revealed that these monokaryons have a relatively high level of genetic diversity. Transfer rates of the EST-SSRs in the monokaryons of closely-related species Pleurotus eryngii var. ferulae and Pleurotus ostreatus were 72% and 64%, respectively. Therefore, our study provides new SSR markers and an efficient method to enhance the crossbreeding of Bailinggu and closely-related species.

  8. Genetic diversity based on SSR markers in maize (Zea mays L ...

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Genetics; Volume 87; Issue 3. Genetic diversity based on SSR markers in maize (Zea mays L.) landraces from Wuling mountain region in China. Yao Qi-Lun Fang Ping Kang Ke-Cheng Pan Guang-Tang. Research Note Volume 87 Issue 3 December 2008 pp 287-291 ...

  9. SSR mining in oil palm EST database: application in oil palm ...

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Genetics; Volume 89; Issue 2. SSR mining in oil palm EST ... Research Article Volume 89 Issue 2 August 2010 pp 135-145 ... The polymerase chain reaction (PCR) products of three primer-pairs detected in E. guineensis, E. oleifera, C. nucifera and Jessinia bataua were cloned and sequenced.

  10. Genetic diversity based on SSR markers in maize (Zea mays L ...

    Indian Academy of Sciences (India)

    be used in maize breeding for improving disease and pest. *For correspondence. E-mail: yql641@yahoo.com.cn. resistance ... DNA isolation and SSR analysis. Genomic DNA was isolated from the third-fresh leaf follow- ... C. The PCR amplification products were separated on 6% (w/v) denatured polyacrylamide gel and vi-.

  11. Analysis of average standardized SSR allele size supports domestication of soybean along the Yellow River

    NARCIS (Netherlands)

    Li, Y.H.; Zhang, C.; Smulders, M.J.M.; Li, W.; Ma, Y.S.; Xu, Qu; Chang, R.Z.; Qiu, Li-Juan

    2013-01-01

    Soybean (Glycine max) was domesticated in China from its wild progenitor G. soja. The geographic region of domestication is, however, not exactly known. Here we employed the directional evolution of SSR (microsatellite) repeats (which mutate preferentially into longer alleles) to analyze the

  12. A high density barley microsatellite consensus map with 775 SSR loci

    NARCIS (Netherlands)

    Varshney, R.K.; Marcel, T.C.; Ramsay, L.; Russell, J.; Roder, M.S.; Stein, N.; Waugh, R.; Langridge, P.; Niks, R.E.; Graner, A.

    2007-01-01

    A microsatellite or simple sequence repeat (SSR) consensus map of barley was constructed by joining six independent genetic maps based on the mapping populations 'Igri x Franka', 'Steptoe x Morex', 'OWBRec x OWBDom', 'Lina x Canada Park', 'L94 x Vada' and 'SusPtrit x Vada'. Segregation data for

  13. APPLICATION OF RYE SSR MARKERS FOR DETECTION OF GENETIC DIVERSITY IN TRITICALE

    Directory of Open Access Journals (Sweden)

    Želmíra Balážová

    2016-06-01

    Full Text Available Present study aims to testify usefulness of particular rye SSR markers for the detection of genetic diversity degree in the set of 20 triticale cultivars coming from different European countries. For this purpose, a set of six rye SSR markers were used. The set of six polymorphic markers provided 22 alleles with an average frequency of 3.67 alleles per locus. The number of alleles ranged between 2 (SCM43 and 5 (SCM28, SCM86. Resulting from the number and frequency of alleles diversity index (DI, polymorphic information content (PIC and probability of identity (PI were calculated. An average value of PIC for 6 SSR markers was 0.505, the highest value was calculated for rye SSR marker SCM86 (0.706. Based on UPGMA algorithm, a dendrogram was constructed. In dendrogram cultivars were divided into two main clusters. The first cluster contained two cultivars, Russian cultivar Greneder and Slovak cultivar Largus, and second included 18 cultivars. Genetically the closest were two Greek cultivars (Niobi and Thisbi and were close to other Greek cultivar Vrodi. It was possible to separate triticale cultivars of spring and winter form in dendrogram. Results showed the utility of rye microsatellite markers for estimation of genetic diversity of European triticale genotypes leading to genotype identification.

  14. Discordant signals of genetic differentiation in the Cannabis genus based on SNP and SSR data

    OpenAIRE

    Henry, Philippe

    2017-01-01

    Recently published studies have identified contrasting patterns of clustering in the Cannabis genus based on Single Nulceotide Polymorphism (SNPs) and microsatellite (SSR) markers. Here we review the differences of previously identified population structure with particular focus on the affinity of NLD, BLD and NLH Cannabis varieties.

  15. Construction of a SNP and SSR linkage map in autotetraploid blueberry using genotyping by sequencing

    Science.gov (United States)

    A mapping population developed from a cross between two key highbush blueberry cultivars, Draper × Jewel (Vaccinium corymbosum), segregating for a number of important phenotypic traits, has been utilized to produce a genetic linkage map. Data on 233 single sequence repeat (SSR) markers and 1794 sing...

  16. Re-conceptualizing mother tongue tuition of Estonian abroad as a transnational phenomenon

    Directory of Open Access Journals (Sweden)

    Maarja Siiner

    2017-05-01

    Full Text Available The governmental initiative called the Compatriots Programme, which supports language tuition in Estonian schools and societies abroad, reveals an increased interest in developing intergenerational language transmission in the growing Estonian diaspora. This transnational language political activity signals a new era in language policy, where nation states are increasingly decentralized by migration. The evaluation of the program furthermore reveals that organizing such schools requires a willingness to take language political agency, typically conducted by well-educated and well-integrated resourceful transnational multilingual parents. The present article outlines the results of an ethnographic study of the process of establishing the Estonian School in Copenhagen. This step has demanded a change in the mindset still prevailing in Estonia that language political activities, such as planning language acquisition, are solely the responsibility of the state. Since the prevailing language ideology in Denmark is not favorable towards multilingualism in migrant languages, intergenerational language transmission furthermore presupposes a feeling of ownership of the language and high language self-esteem. "Hargmaise keelepoliitika sünd. Eesti keeleõppe korraldamise võimalikkusest välismaal Taani näitel" Hargmaisus, kasvav väljarändajate arv ja sellega ka eesti keele rääkijate hulk välismaal on jätnud oma jälje eesti keelepoliitikale. Kui varem uuriti peamiselt seda, kuidas eesti keel muukeelses kontekstis muutub, siis viimasel kümnendil on riik asunud aktiivselt toetama eesti keele jätkuvat kasutamist välismaal, rahastades rahvuskaaslaste programmi abil haridusprogramme. Kuid millised faktorid määravad selle, kas uus eestlaste põlvkond oskab ja tahab eesti keelt rääkida? Artikkel hindab etnograafilises ja sotsiolingvistilises võtmes Kopenhaagenis kolm aastat tegutsenud Eesti Kooli ja Lasteklubi näitel, millised on keelekasutust

  17. A simple ssr analysis for genetic diversity estimation of maize landraces

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    Ignjatovic-Micic Dragana

    2015-01-01

    Full Text Available collection of 2217 landraces from western Balkan (former Yugoslavia is maintained at Maize Research Institute Zemun Polje gene bank. Nine flint and nine dent accessions from six agro-ecological groups (races, chosen on the basis of diverse pedigrees, were analyzed for genetic relatedness using phenotypic and simple sequence repeat (SSR markers. One of the aims was to establish a reliable set of SSR markers for a rapid diversity analysis using polyacrilamide gels and ethidium bromide staining. In the principal component analysis (PCA the first three principal components accounted for 80.86% of total variation and separated most of the flint from dent landraces. Ten SSR primers revealed a total of 56 and 63 alleles in flint and dent landraces, respectively, with low stuttering and good allele resolution on the gels. High average PIC value (0.822 also supports informativeness and utility of the markers used in this study. Higher genetic variation was observed among flint genotypes, as genetic distances between flint landraces covered a larger range of values (0.11- 0.38 than between dent (0.22 - 0.33 genotypes. Both phenotypic and SSR analyses distinguished flint and dent landraces, but neither of them could abstract agro-ecological groups. The SSR method used gave clear, easy to read band patterns that could be used for reliable allele frequency determination. Genetic diversity revealed for both markers indicated that the landraces were highly adapted to specific environmental conditions and purposes and could be valuable sources of genetic variability. [Projekat Ministarstva nauke Republike Srbije, br. TR31028: Exploitation of maize diversity to improve grain quality and drought tolerance

  18. Characterization and compilation of polymorphic simple sequence repeat (SSR markers of peanut from public database

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    Zhao Yongli

    2012-07-01

    Full Text Available Abstract Background There are several reports describing thousands of SSR markers in the peanut (Arachis hypogaea L. genome. There is a need to integrate various research reports of peanut DNA polymorphism into a single platform. Further, because of lack of uniformity in the labeling of these markers across the publications, there is some confusion on the identities of many markers. We describe below an effort to develop a central comprehensive database of polymorphic SSR markers in peanut. Findings We compiled 1,343 SSR markers as detecting polymorphism (14.5% within a total of 9,274 markers. Amongst all polymorphic SSRs examined, we found that AG motif (36.5% was the most abundant followed by AAG (12.1%, AAT (10.9%, and AT (10.3%.The mean length of SSR repeats in dinucleotide SSRs was significantly longer than that in trinucleotide SSRs. Dinucleotide SSRs showed higher polymorphism frequency for genomic SSRs when compared to trinucleotide SSRs, while for EST-SSRs, the frequency of polymorphic SSRs was higher in trinucleotide SSRs than in dinucleotide SSRs. The correlation of the length of SSR and the frequency of polymorphism revealed that the frequency of polymorphism was decreased as motif repeat number increased. Conclusions The assembled polymorphic SSRs would enhance the density of the existing genetic maps of peanut, which could also be a useful source of DNA markers suitable for high-throughput QTL mapping and marker-assisted selection in peanut improvement and thus would be of value to breeders.

  19. RAPD and SSR Polymorphisms in Mutant Lines of Transgenic Wheat Mediated by Low Energy Ion Beam

    International Nuclear Information System (INIS)

    Wang Tiegu; Huang Qunce; Feng Weisen

    2007-01-01

    Two types of markers-random amplified polymorphic DNA (RAPD) and simple sequence repeat DNA (SSR)-have been used to characterize the genetic diversity among nine mutant lines of transgenic wheat intermediated by low energy ion beam and their four receptor cultivars. The objectives of this study were to analyze RAPD-based and SSR-based genetic variance among transgenic wheat lines and with their receptors, and to find specific genetic markers of special traits of transgenic wheat lines. 170 RAPD primers were amplified to 733 fragments in all the experimental materials. There were 121 polymorphic fragments out of the 733 fragments with a ratio of polymorphic fragments of 16.5%. 29 SSR primer pairs were amplified to 83 fragments in all the experiment materials. There were 57 polymorphic fragments out of the 83 fragments with a ratio of polymorphic fragments of 68.7%. The dendrograms were prepared based on a genetic distance matrix using the UPGMA (Unweighted Pair-group Method with Arithmetic averaging) algorithm, which corresponded well to the results of the wheat pedigree analysis and separated the 13 genotypes into four groups. Association analysis between RAPD and SSR markers with the special traits of transgenic wheat mutant lines discovered that three RAPD markers, s1, opt-16, and f14, were significantly associated with the muticate trait, while three SSR markers, Rht8 (Xgwm261), Rht-B1b, and Rht-D1b, highly associated with the dwarf trait. These markers will be useful for marker-assistant breeding and can be used as candidate markers for further gene mapping and cloning

  20. Grammar School Students’ Opinions on the Art Curriculum: An Estonian, Portuguese and Slovenian Comparative Study

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    Tomaž Zupančič

    2015-09-01

    Full Text Available The article presents the attitude of grammar school students towards the art curriculum. It first provides an overview of the characteristics of contemporary art education, with an emphasis on the postmodern art curriculum and on linking course content with students’ interests. The study is based on the descriptive and causal non-experimental method, with a sample comprising 387 Slovenian, Estonian and Portuguese students. It was established that the students place the highest value on developing creativity, and are less interested in art history content and learning about the basics of the formal art language. They are attracted to contemporary topics, such as graffiti, multicultural art, the use of new media, and digital technologies. The results of the study provide opportunities for future comparative analyses and starting points for updating art curricula.

  1. Role of fatty acids in the formation of Estonian oil shale (kukersite)

    Energy Technology Data Exchange (ETDEWEB)

    Klesment, I.

    1973-01-01

    Study of the composition of the tar from semicoking of Estonian kukersite, by separation and chromatographic and spectroscopic analysis, showed that approximately 40 percent of the carbon of the bituminous material existed as linear chains. At the beginning of fossilization the original biological material for the formation of the kerogen of the kukersite (saturated and unsaturated fatty acids C/sub 14/-C/sub 18/) was oxidized at the double bonds of the unsaturated acids, and carbonyl and ..beta..-polycarbonyl groups were formed. During pyrolysis of the polycarbonyl chains, alkylresorcinols were formed containing linear alkyl chains with less than or equal to 10 carbon atoms. The phenols, boiling at 280/sup 0/-430/sup 0/C, have a much higher alkylresorcinols content than those obtained during semicoking of other solid fuels.

  2. Comparative analysis of idiom selection and sequencing 5 in Estonian basic school EFL coursebooks

    Directory of Open Access Journals (Sweden)

    Rita Anita Forssten

    2017-05-01

    Full Text Available The article investigates the selection and sequencing of the idioms encountered in two locally-produced and international coursebook series currently employed in Estonian basic schools. It is hypothesized that there exists a positive correlation between idioms’ difficulty and coursebooks’ language proficiency level. The hypothesis is tested through a statistical analysis of the idioms found which are categorized in terms of their analysability into three categories where category 1 includes analysable semi-literal idioms, category 2 comprises analysable semi-transparent idioms, and category 3 encompasses non-analysable opaque idioms, and then analysed through an online language corpus (British National Corpus. The results of the study reveal that the coursebook authors under discussion have disregarded idioms’ frequency as a criterion for selection or sequencing, whereas the factor utilized to some extent is the degree of analysability.

  3. Whole-word frequency and inflectional paradigm size facilitate Estonian case-inflected noun processing.

    Science.gov (United States)

    Lõo, Kaidi; Järvikivi, Juhani; Baayen, R Harald

    2018-06-01

    Estonian is a morphologically rich Finno-Ugric language with nominal paradigms that have at least 28 different inflected forms but sometimes more than 40. For languages with rich inflection, it has been argued that whole-word frequency, as a diagnostic of whole-word representations, should not be predictive for lexical processing. We report a lexical decision experiment, showing that response latencies decrease both with frequency of the inflected form and its inflectional paradigm size. Inflectional paradigm size was also predictive of semantic categorization, indicating it is a semantic effect, similar to the morphological family size effect. These findings fit well with the evidence for frequency effects of word n-grams in languages with little inflectional morphology, such as English. Apparently, the amount of information on word use in the mental lexicon is substantially larger than was previously thought. Copyright © 2018 Elsevier B.V. All rights reserved.

  4. [Kreem, Juhan. Sketches of a Clerk. Pen-and-ink Drawings in the Margins of the Medieval Accoung Books of Reval (Tallinn)] / Anu Mänd

    Index Scriptorium Estoniae

    Mänd, Anu, 1968-

    2008-01-01

    Arvustus: Kreem, Juhan. Sketches of a Clerk. Pen-and-ink Drawings in the Margins of the Medieval Accoung Books of Reval (Tallinn). (Krems, 2006). Juhan Kreemi raamat käsitleb Tallinna rae keskaegsetes arveraamatutes leiduvaid sulejoonistusi. Autor on välja selgitanud nende, esmapilgul juhuslikena näivate joonistuste otstarbe ning püüdnud nende kaudu avada tolleaegsete linnaametnike mõttemalle

  5. Chromosomal microarray analysis as a first-tier clinical diagnostic test: Estonian experience.

    Science.gov (United States)

    Zilina, Olga; Teek, Rita; Tammur, Pille; Kuuse, Kati; Yakoreva, Maria; Vaidla, Eve; Mölter-Väär, Triin; Reimand, Tiia; Kurg, Ants; Ounap, Katrin

    2014-03-01

    Chromosomal microarray analysis (CMA) is now established as the first-tier cytogenetic diagnostic test for fast and accurate detection of chromosomal abnormalities in patients with developmental delay/intellectual disability (DD/ID), multiple congenital anomalies (MCA), and autism spectrum disorders (ASD). We present our experience with using CMA for postnatal and prenatal diagnosis in Estonian patients during 2009-2012. Since 2011, CMA is on the official service list of the Estonian Health Insurance Fund and is performed as the first-tier cytogenetic test for patients with DD/ID, MCA or ASD. A total of 1191 patients were analyzed, including postnatal (1072 [90%] patients and 59 [5%] family members) and prenatal referrals (60 [5%] fetuses). Abnormal results were reported in 298 (25%) patients, with a total of 351 findings (1-3 per individual): 147 (42%) deletions, 106 (30%) duplications, 89 (25%) long contiguous stretches of homozygosity (LCSH) events (>5 Mb), and nine (3%) aneuploidies. Of all findings, 143 (41%) were defined as pathogenic or likely pathogenic; for another 143 findings (41%), most of which were LCSH, the clinical significance remained unknown, while 61 (18%) reported findings can now be reclassified as benign or likely benign. Clinically relevant findings were detected in 126 (11%) patients. However, the proportion of variants of unknown clinical significance was quite high (41% of all findings). It seems that our ability to detect chromosomal abnormalities has far outpaced our ability to understand their role in disease. Thus, the interpretation of CMA findings remains a rather difficult task requiring a close collaboration between clinicians and cytogeneticists.

  6. Identification, evaluation, and application of the genomic-SSR loci in ramie

    Directory of Open Access Journals (Sweden)

    Ming-Bao Luan

    2016-09-01

    Full Text Available To provide a theoretical and practical foundation for ramie genetic analysis, simple sequence repeats (SSRs were identified in the ramie genome and employed in this study. From the 115 369 sequences of a specific-locus amplified fragment library, a type of reduced representation library obtained by high-throughput sequencing, we identified 4774 sequences containing 5064 SSR motifs. SSRs of ramie included repeat motifs with lengths of 1 to 6 nucleotides, and the abundance of each motif type varied greatly. We found that mononucleotide, dinucleotide, and trinucleotide repeat motifs were the most prevalent (95.91%. A total of 98 distinct motif types were detected in the genomic-SSRs of ramie. Of them, The A/T mononucleotide motif was the most abundant, accounting for 41.45% of motifs, followed by AT/TA, accounting for 20.30%. The number of alleles per locus in 31 polymorphic microsatellite loci ranged from 2 to 7, and observed and expected heterozygosities ranged from 0.04 to 1.00 and 0.04 to 0.83, respectively. Furthermore, molecular identity cards (IDs of the germplasms were constructed employing the ID Analysis 3.0 software. In the current study, the 26 germplasms of ramie can be distinguished by a combination of five SSR primers including Ibg5-5, Ibg3-210, Ibg1-11, Ibg6-468, and Ibg6-481. The allele polymorphisms produced by all SSR primers were used to analyze genetic relationships among the germplasms. The similarity coefficients ranged from 0.41 to 0.88. We found that these 26 germplasms were clustered into five categories using UPGMA, with poor correlation between germplasm and geographical distribution. Our study is the first large-scale SSR identification from ramie genomic sequences. We have further studied the SSR distribution pattern in the ramie genome, and proposed that it is possible to develop SSR loci from genomic data for population genetics studies, linkage mapping, quantitative trait locus mapping, cultivar fingerprinting

  7. Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L.) A. Rich

    Science.gov (United States)

    Chankaew, Sompong; Kaga, Akito; Naito, Ken; Ehara, Hiroshi; Tomooka, Norihiko

    2015-01-01

    Vigna vexillata (L.) A. Rich. (tuber cowpea) is an underutilized crop for consuming its tuber and mature seeds. Wild form of V. vexillata is a pan-tropical perennial herbaceous plant which has been used by local people as a food. Wild V. vexillata has also been considered as useful gene(s) source for V. unguiculata (cowpea), since it was reported to have various resistance gene(s) for insects and diseases of cowpea. To exploit the potential of V. vexillata, an SSR-based linkage map of V. vexillata was developed. A total of 874 SSR markers successfully amplified single DNA fragment in V. vexillata among 1,336 SSR markers developed from Vigna angularis (azuki bean), V. unguiculata and Phaseolus vulgaris (common bean). An F2 population of 300 plants derived from a cross between salt resistant (V1) and susceptible (V5) accessions was used for mapping. A genetic linkage map was constructed using 82 polymorphic SSR markers loci, which could be assigned to 11 linkage groups spanning 511.5 cM in length with a mean distance of 7.2 cM between adjacent markers. To develop higher density molecular linkage map and to confirm SSR markers position in a linkage map, RAD markers were developed and a combined SSR and RAD markers linkage map of V. vexillata was constructed. A total of 559 (84 SSR and 475 RAD) markers loci could be assigned to 11 linkage groups spanning 973.9 cM in length with a mean distance of 1.8 cM between adjacent markers. Linkage and genetic position of all SSR markers in an SSR linkage map were confirmed. When an SSR genetic linkage map of V. vexillata was compared with those of V. radiata and V. unguiculata, it was suggested that the structure of V. vexillata chromosome was considerably differentiated. This map is the first SSR and RAD marker-based V. vexillata linkage map which can be used for the mapping of useful traits. PMID:26398819

  8. Characterization and Amplification of Gene-Based Simple Sequence Repeat (SSR) Markers in Date Palm.

    Science.gov (United States)

    Zhao, Yongli; Keremane, Manjunath; Prakash, Channapatna S; He, Guohao

    2017-01-01

    The paucity of molecular markers limits the application of genetic and genomic research in date palm (Phoenix dactylifera L.). Availability of expressed sequence tag (EST) sequences in date palm may provide a good resource for developing gene-based markers. This study characterizes a substantial fraction of transcriptome sequences containing simple sequence repeats (SSRs) from the EST sequences in date palm. The EST sequences studied are mainly homologous to those of Elaeis guineensis and Musa acuminata. A total of 911 gene-based SSR markers, characterized with functional annotations, have provided a useful basis not only for discovering candidate genes and understanding genetic basis of traits of interest but also for developing genetic and genomic tools for molecular research in date palm, such as diversity study, quantitative trait locus (QTL) mapping, and molecular breeding. The procedures of DNA extraction, polymerase chain reaction (PCR) amplification of these gene-based SSR markers, and gel electrophoresis of PCR products are described in this chapter.

  9. Genetic similarity among Tunisian olive cultivars and two unknown feral olive trees estimated through SSR markers.

    Science.gov (United States)

    Ben-Ayed, Rayda; Sans-Grout, Cinderella; Moreau, Fabienne; Grati-Kamoun, Naziha; Rebai, Ahmed

    2014-06-01

    We used eight informative microsatellite markers for fingerprinting and evaluation of genetic similarity among 15 Tunisian olive (Olea europaea L.) cultivars and two feral unknown trees named Soulela 1 and Soulela 2. Thirty-one alleles were revealed, and the number of alleles per SSR varied from 2 (UDO12) to 6 (GAPU71A). Cluster analysis grouped cultivars into three main clusters. The two unknown varieties could not be reliably classified into any of these cultivar groups. SSR analysis indicated the presence of three erroneous denominations of cultivars. We resolved two synonymy cases (Zalmati and Chemlali; Rkhami and Chetoui) and one case of homonymy (Chemlali Tataouine). Genetic analyses of DNA extracted from leaves, oils, and embryos of the two unknown cultivars and the two major Tunisian olive cultivars (Chemlali and Chetoui) were also studied. We conclude that the reliable identification of these two feral cultivars needs to be addressed by a larger set of markers.

  10. Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species

    Directory of Open Access Journals (Sweden)

    María F. Pomponio

    2015-08-01

    Full Text Available Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus. Area of the study: samples were obtained from natural populations of Argentina. Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P.alba, P. denudans, P. hassleriP. chilensis, P. flexuosa, and interspecific hybrids. Main results: In P. alba, the PIC average value was 0.36; and 6 out of the 11 primers showed high values of polymorphism ranging from 0.40 to 0.71. The cross-species transferability was high with high percentages of polymorphic loci. Research highlights: The SSR markers developed in P.alba were easily transferred to other Prosopis species which did not have functional markers.

  11. Development and validation of genic-SSR markers in sesame by RNA-seq

    Science.gov (United States)

    2012-01-01

    Background Sesame (Sesamum indicum L.) is one of the most important oil crops; however, a lack of useful molecular markers hinders current genetic research. We performed transcriptome sequencing of samples from different sesame growth and developmental stages, and mining of genic-SSR markers to identify valuable markers for sesame molecular genetics research. Results In this study, 75 bp and 100 bp paired-end RNA-seq was used to sequence 24 cDNA libraries, and 42,566 uni-transcripts were assembled from more than 260 million filtered reads. The total length of uni-transcript sequences was 47.99 Mb, and 7,324 SSRs (SSRs ≥15 bp) and 4,440 SSRs (SSRs ≥18 bp) were identified. On average, there was one genic-SSR per 6.55 kb (SSRs ≥15 bp) or 10.81 kb (SSRs ≥18 bp). Among perfect SSRs (≥18 bp), di-nucleotide motifs (48.01%) were the most abundant, followed by tri- (20.96%), hexa- (25.37%), penta- (2.97%), tetra- (2.12%), and mono-nucleotides (0.57%). The top four motif repeats were (AG/CT)n [1,268 (34.51%)], (CA/TG)n [281 (7.65%)], (AT/AT)n [215 (5.85%)], and (GAA/TTC)n [131 (3.57%)]. A total of 2,164 SSR primer pairs were identified in the 4,440 SSR-containing sequences (≥18 bp), and 300 SSR primer pairs were randomly chosen for validation. These SSR markers were amplified and validated in 25 sesame accessions (24 cultivated accessions, one wild species). 276 (92.0%) primer pairs yielded PCR amplification products in 24 cultivars. Thirty two primer pairs (11.59%) exhibited polymorphisms. Moreover, 203 primer pairs (67.67%) yielded PCR amplicons in the wild accession and 167 (60.51%) were polymorphic between species. A UPGMA dendrogram based on genetic similarity coefficients showed that the correlation between genotype and geographical source was low and that the genetic basis of sesame in China is narrow, as previously reported. The 32 polymorphic primer pairs were validated using an F2 mapping population; 18 primer pairs exhibited

  12. Mining and Development of Novel SSR Markers Using Next Generation Sequencing (NGS Data in Plants

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    Sima Taheri

    2018-02-01

    Full Text Available Microsatellites, or simple sequence repeats (SSRs, are one of the most informative and multi-purpose genetic markers exploited in plant functional genomics. However, the discovery of SSRs and development using traditional methods are laborious, time-consuming, and costly. Recently, the availability of high-throughput sequencing technologies has enabled researchers to identify a substantial number of microsatellites at less cost and effort than traditional approaches. Illumina is a noteworthy transcriptome sequencing technology that is currently used in SSR marker development. Although 454 pyrosequencing datasets can be used for SSR development, this type of sequencing is no longer supported. This review aims to present an overview of the next generation sequencing, with a focus on the efficient use of de novo transcriptome sequencing (RNA-Seq and related tools for mining and development of microsatellites in plants.

  13. [Development of new SSR markers from EST of SSH cDNA libraries on rose fragrance].

    Science.gov (United States)

    Yan, Hui-Jun; Zhang, Hao; Xie, Ji-Rong; Li, Shu-Fa; Jian, Hong-Ying; Qiu, Xian-Qin; Wang, Qi-Gang; Wang, Ji-Hua; Tang, Kai-Xue

    2009-09-01

    The new SSR markers of rose related fragrance were developed based on the SSH cDNA libraries of rose floral scent mutant. In this study, 10 EST-SSRs (2.6%) from 391 ESTs in the libraries were identified. Six EST-SSRs primers were designed to sequence flanking SSRs. The primer pairs designed were screened on the wild-type Jinyindao, which has flowers full of pleasant scent, and the mutant-type Wangriqinghuai without perceivable floral scent. Five primer pairs were amplified effectively in Jinyindao and Wangriqinghuai, and 3 were polymorphic between Jinyindao and Wangriqinghuai. Eighteen rose cultivars including fragrant roses and nonfragrant roses were identified by the five prime pairs. These results proved that EST-SSR markers are effective markers to identify the polymorphism of the rose.

  14. Kolm Eesti Robinsoni: Daniel Defoe romaan eesti tõlkes / Three Estonian Robinsons: Daniel Defoe’s Novel in Estonian Translation

    Directory of Open Access Journals (Sweden)

    Ene-Reet Soovik

    2012-06-01

    Full Text Available The article discusses three Estonian translations of Daniel Defoe’s Robinson Crusoe into Estonian with the focus on the completeness of the translated texts and the characterisation given to these in paratextual information. While there are several translations and versions of the tex t available in E stonian that have either used a mediating language or do not proceed directly from Defoe’s novel, three editions explicitly list Defoe’s English-language Robinson Crusoe as their source text. These are Rudolf Sirge’s translation from 1950 and two editions translated by Valter Rummel that appeared in 1984 (reprinted in 2001 and 2007, respectively. The article sets out to discover the main differences between the three editions and the possible reasons that may have triggered their publication in Estonia at those particular times. In order to approach the issues, a general framework derived from descriptive translation studies is employed with an emphasis on Gideon Toury’s chrestomatic treatment of translation norms. Thus an attempt is made to detect the preliminary translational norms regarding translation policy, particularly the choice of texts to be translated, as well as the matricial norms that concern the fullness of the translated text and are part of operational norms manifested in the translator’s decisions which, in two of the cases at hand, may also have been decisions made by the editor or the censor. Rudolf Sirge’s translation appeared at a time when Estonia had fairly recently been incorporated into the Soviet Union and there was a lack of children’s literature ideologically appreciated by the regime. This may account for the packaging of the book as a work with a strong didactic bent, while its primary audience was taken to be children and young adults for whom the protagonist served as an example of a hard-working and tenacious hero to be emulated by young Soviets. The target text has been considerably shortened as

  15. Genetic diversity of Phytophthora sojae isolates in Heilongjiang Province in China assessed by RAPD and EST-SSR

    Science.gov (United States)

    Wu, J. J.; Xu, P. F.; Liu, L. J.; Wang, J. S.; Lin, W. G.; Zhang, S. Z.; Wei, L.

    Random-amplified polymorphic DNA (RAPD) and EST-SSR markers were used to estimate the genetic relationship among thirty-nine P.sojae isolates from three locations in Heilongjiang Province, and nine isolates from Ohio in America were made as reference strains. 10 of 50 RAPD primers and 5 of 33 EST-SSR were polymorphic across 48 P.sojae isolates. Similarity values among P.sojae isolates were from 49% to 82% based on the RAPD data. The similarities based on EST-SSR markers ranged from 47% to 85%. The genetic diversity revealed by EST-SSR marker analysis was higher than that obtained from RAPD. The similarity matrices for the SSR data and the RAPD data were moderately correlated (r = 0.47). Genetic similarity coefficients were also relatively lower, which demonstrated complicated genetic background within each location. The high similarity values range revealed the ability of RAPD/EST-SSR markers to distinguish even among morphological similar phytophthora.

  16. Estonian exceptionalism

    Index Scriptorium Estoniae

    2011-01-01

    Ulatuslikud kärped ja liitumine eurotsooniga toovad Eestist häid majandusuudiseid: töötus langeb, majandus kasvab, eksport tõuseb, eelarve on plussis, reitinguagentuur Fitch krediidireiting tõusis tasemele A+

  17. Characterization of novel SSR markers in diverse sainfoin (Onobrychis viciifolia) germplasm.

    Science.gov (United States)

    Kempf, Katharina; Mora-Ortiz, Marina; Smith, Lydia M J; Kölliker, Roland; Skøt, Leif

    2016-08-30

    Sainfoin is a perennial forage legume with beneficial properties for animal husbandry due to the presence of secondary metabolites. However, worldwide cultivation of sainfoin is marginal due to the lack of varieties with good agronomic performance, adapted to a broad range of environmental conditions. Little is known about the genetics of sainfoin and only few genetic markers are available to assist breeding and genetic investigations. The objective of this study was to develop a set of SSR markers useful for genetic studies in sainfoin and their characterization in diverse germplasm. A set of 400 SSR primer combinations were tested for amplification and their ability to detect polymorphisms in a set of 32 sainfoin individuals, representing distinct varieties or landraces. Alleles were scored for presence or absence and polymorphism information content of each SSR locus was calculated with an adapted formula taking into account the tetraploid character of sainfoin. Relationships among individuals were visualized using cluster and principle components analysis. Of the 400 primer combinations tested, 101 reliably detected polymorphisms among the 32 sainfoin individuals. Among the 1154 alleles amplified 250 private alleles were observed. The number of alleles per locus ranged from 2 to 24 with an average of 11.4 alleles. The average polymorphism information content reached values of 0.14 to 0.36. The clustering of the 32 individuals suggested a separation into two groups depending on the origin of the accessions. The SSR markers characterized and tested in this study provide a valuable tool to detect polymorphisms in sainfoin for future genetic studies and breeding programs. As a proof of concept, we showed that these markers can be used to separate sainfoin individuals based on their origin.

  18. Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species

    OpenAIRE

    María F. Pomponio; Cintia Acuña; Vivien Pentreath; Diego L. Lauenstein; Susana M. Poltri; Susana Torales

    2015-01-01

    Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus. Area of the study: samples were obtained from natural populations of Argentina. Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P.alba, P. denudans, P. hassleriP. chilensis, P. flexuosa, and interspecific hybrids. Main res...

  19. Genetic variation of european maize genotypes (zea mays l. Detected using ssr markers

    Directory of Open Access Journals (Sweden)

    Martin Vivodík

    2017-01-01

    Full Text Available The SSR molecular markers were used to assess genetic diversity in 40 old European maize genotypes. Ten SSR primers revealed a total of 65 alleles ranging from 4 (UMC1060 to 8 (UMC2002 and UMC1155 alleles per locus with a mean value of 6.50 alleles per locus. The PIC values ranged from 0.713 (UMC1060 to 0.842 (UMC2002 with an average value of 0.810 and the DI value ranged from 0.734 (UMC1060 to 0.848 (UMC2002 with an average value of 0.819. 100% of used SSR markers had PIC and DI values higher than 0.7 that means high polymorphism of chosen markers used for analysis. Probability of identity (PI was low ranged from 0.004 (UMC1072 to 0.022 (UMC1060 with an average of 0.008. A dendrogram was constructed from a genetic distance matrix based on profiles of the 10 maize SSR loci using the unweighted pair-group method with the arithmetic average (UPGMA. According to analysis, the collection of 40 diverse accessions of maize was clustered into four clusters. The first cluster contained nine genotypes of maize, while the second cluster contained the four genotypes of maize. The third cluster contained 5 maize genotypes. Cluster 4 contained five genotypes from Hungary (22.73%, two genotypes from Poland (9.10%, seven genotypes of maize from Union of Soviet Socialist Republics (31.81%, six genotypes from Czechoslovakia (27.27%, one genotype from Slovak Republic (4.55% and one genotype of maize is from Yugoslavia (4.55%. We could not distinguish 4 maize genotypes grouped in cluster 4, (Voroneskaja and Kocovska Skora and 2 Hungarian maize genotypes - Feheres Sarga Filleres and Mindszentpusztai Feher, which are genetically the closest.

  20. Phylogeny and sequence variability of the Sarcocystis singaporensis Zaman and Colley, (1975) 1976 ssrDNA

    Czech Academy of Sciences Publication Activity Database

    Šlapeta, Jan Roger; Kyselová, Iveta; Richardson, A. O.; Modrý, David; Lukeš, Julius

    2002-01-01

    Roč. 88, č. 9 (2002), s. 810-815 ISSN 0932-0113 R&D Projects: GA ČR GA524/00/P015; GA AV ČR KSK6005114 Institutional research plan: CEZ:AV0Z6022909 Keywords : Sarcocystis * phylogeny * ssrDNA Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.046, year: 2002

  1. Use of genome sequence data in the design and testing of SSR markers for Phytophthora species.

    Science.gov (United States)

    Schena, Leonardo; Cardle, Linda; Cooke, David E L

    2008-12-19

    Microsatellites or single sequence repeats (SSRs) are a powerful choice of marker in the study of Phytophthora population biology, epidemiology, ecology, genetics and evolution. A strategy was tested in which the publicly available unigene datasets extracted from genome sequences of P. infestans, P. sojae and P. ramorum were mined for candidate SSR markers that could be applied to a wide range of Phytophthora species. A first approach, aimed at the identification of polymorphic SSR loci common to many Phytophthora species, yielded 171 reliable sequences containing 211 SSRs. Microsatellites were identified from 16 target species representing the breadth of diversity across the genus. Repeat number ranged from 3 to 16 with most having seven repeats or less and four being the most commonly found. Trinucleotide repeats such as (AAG)n, (AGG)n and (AGC)n were the most common followed by pentanucleotide, tetranucleotide and dinucleotide repeats. A second approach was aimed at the identification of useful loci common to a restricted number of species more closely related to P. sojae (P. alni, P. cambivora, P. europaea and P. fragariae). This analysis yielded 10 trinucleotide and 2 tetranucleotide SSRs which were repeated 4, 5 or 6 times. Key studies on inter- and intra-specific variation of selected microsatellites remain. Despite the screening of conserved gene coding regions, the sequence diversity between species was high and the identification of useful SSR loci applicable to anything other than the most closely related pairs of Phytophthora species was challenging. That said, many novel SSR loci for species other than the three 'source species' (P. infestans, P. sojae and P. ramorum) are reported, offering great potential for the investigation of Phytophthora populations. In addition to the presence of microsatellites, many of the amplified regions may represent useful molecular marker regions for other studies as they are highly variable and easily amplifiable from

  2. DEVELOPMENT OF EST-SSR MARKERS TO ASSESS GENETIC DIVERSITY OF BROCCOLI AND ITS RELATED SPECIES

    Directory of Open Access Journals (Sweden)

    Nur Kholilatul Izzah

    2017-01-01

    Full Text Available Development of Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR markers derived from public database is known to be more efficient, faster and low cost. The objective of this study was to generate a new set of EST-SSR markers for broccoli and its related species and their usefulness for assessing their genetic diversity. A total of 202 Brassica oleracea ESTs were retrieved from NCBI and then assembled into 172 unigenes by means of CAP3 program. Identification of SSRs was carried out using web-based tool, RepeatMasker software. Afterwards, EST-SSR markers were developed using Primer3 program. Among the identified SSRs, trinucleotide repeats were the most common repeat types, which accounted for about 50%. A total of eight primer pairs were successfully designed and yielded amplification products. Among them, five markers were polymorphic and displayed a total of 30 alleles with an average number of six alleles per locus. The polymorphic markers were subsequently used for analyzing genetic diversity of 36 B. oleracea cultivars including 22 broccoli, five cauliflower and nine kohlrabi cultivars based on genetic similarity matrix as implemented in NTSYS program. At similarity coefficient of 61%, a UPGMA clustering dendrogram effectively separated 36 genotypes into three main groups, where 30 out of 36 genotypes were clearly discriminated. The result obtained in the present study would help breeders in selecting parental lines for crossing. Moreover, the novel EST-SSR markers developed in the study could be a valuable tool for differentiating cultivars of broccoli and related species.

  3. Assessment of genetic diversity of silver fir (Abies alba Mill. in Serbia using SSR markers

    Directory of Open Access Journals (Sweden)

    Popović Vladan

    2017-01-01

    Full Text Available The paper presents the results of the analysis of genetic variability of eight populations of silver fir (Abies alba Mill. in Serbia obtained using SSR markers. The genomic DNA was isolated from tissue of needles of all eight populations. Due to the costly and lengthy process a small number of the SSR markers for Abies alba have been developed, so in this study were used the microsatellite markers of related species. The obtained results indicate a low level of the genetic variability between natural populations of silver fir. The total number of alleles detected with nine SSR markers in eight studied populations of silver fir is 28. The range of alleles varies from two for NFF15 to six for SF78 with an average of 3.1 alleles per locus. The mean value of genetic similarity between populations is 0.92. The smallest genetic similarity between pairs of populations is 0.82 (Dubočica Bare and Stara Planina; Dubočica Bare and Tara and the greatest genetic similarity is 1 (Zlatar and Stara Planina, Zlatar and Tara, Stara Planina and Tara. A basic insight into the level of genetic diversity of natural populations of silver fir in Serbia, which are located in a relatively small area, has been given using a set of SSR markers. The obtained results can be used in the future strategy for the management and regeneration of silver fir forests. [[Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR 31070: The developments of technological procedures in forestry in order to attain an optimal forest cover percentage

  4. Science and Technology in the Latvian SSR on its Twentieth Anniversary

    Science.gov (United States)

    1961-10-18

    the way to the use or low percentage ores, naturalŕ brine and Industrial effluents . Resean and his colleagues have studied over thirty hitherto...introduced herbaceous tannin - bearing plants has shown that in the conditions of the Latvian S.S.R. in terms of yield and content of tannides in...their roots the mot promising species for introduction into lture are taran tannin and Tsien-Chan dock. On the basis Df the investigation, instructions

  5. Use of genome sequence data in the design and testing of SSR markers for Phytophthora species

    Directory of Open Access Journals (Sweden)

    Cardle Linda

    2008-12-01

    Full Text Available Abstract Background Microsatellites or single sequence repeats (SSRs are a powerful choice of marker in the study of Phytophthora population biology, epidemiology, ecology, genetics and evolution. A strategy was tested in which the publicly available unigene datasets extracted from genome sequences of P. infestans, P. sojae and P. ramorum were mined for candidate SSR markers that could be applied to a wide range of Phytophthora species. Results A first approach, aimed at the identification of polymorphic SSR loci common to many Phytophthora species, yielded 171 reliable sequences containing 211 SSRs. Microsatellites were identified from 16 target species representing the breadth of diversity across the genus. Repeat number ranged from 3 to 16 with most having seven repeats or less and four being the most commonly found. Trinucleotide repeats such as (AAGn, (AGGn and (AGCn were the most common followed by pentanucleotide, tetranucleotide and dinucleotide repeats. A second approach was aimed at the identification of useful loci common to a restricted number of species more closely related to P. sojae (P. alni, P. cambivora, P. europaea and P. fragariae. This analysis yielded 10 trinucleotide and 2 tetranucleotide SSRs which were repeated 4, 5 or 6 times. Conclusion Key studies on inter- and intra-specific variation of selected microsatellites remain. Despite the screening of conserved gene coding regions, the sequence diversity between species was high and the identification of useful SSR loci applicable to anything other than the most closely related pairs of Phytophthora species was challenging. That said, many novel SSR loci for species other than the three 'source species' (P. infestans, P. sojae and P. ramorum are reported, offering great potential for the investigation of Phytophthora populations. In addition to the presence of microsatellites, many of the amplified regions may represent useful molecular marker regions for other studies as

  6. Genetic diversity in watermelon (Citrullus lanatus) landraces from Zimbabwe revealed by RAPD and SSR markers.

    Science.gov (United States)

    Mujaju, C; Sehic, J; Werlemark, G; Garkava-Gustavsson, L; Fatih, M; Nybom, H

    2010-08-01

    Low polymorphism in cultivated watermelon has been reported in previous studies, based mainly on US Plant Introductions and watermelon cultivars, most of which were linked to breeding programmes associated with disease resistance. Since germplasm sampled in a putative centre of origin in southern Africa may harbour considerably higher variability, DNA marker-based diversity was estimated among 81 seedlings from eight accessions of watermelon collected in Zimbabwe; five accessions of cow-melons (Citrullus lanatus var. citroides) and three of sweet watermelons (C. lanatus var. lanatus). Two molecular marker methods were used, random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) also known as microsatellite DNA. Ten RAPD primers produced 138 markers of which 122 were polymorphic. Nine SSR primer pairs detected a total of 43 alleles with an average of 4.8 alleles per locus. The polymorphic information content (PIC) ranged from 0.47 to 0.77 for the RAPD primers and from 0.39 to 0.97 for the SSR loci. Similarity matrices obtained with SSR and RAPD, respectively, were highly correlated but only RAPD was able to provide each sample with an individual-specific DNA profile. Dendrograms and multidimensional scaling (MDS) produced two major clusters; one with the five cow-melon accessions and the other with the three sweet watermelon accessions. One of the most variable cow-melon accessions took an intermediate position in the MDS analysis, indicating the occurrence of gene flow between the two subspecies. Analysis of molecular variation (AMOVA) attributed most of the variability to within-accessions, and contrary to previous reports, sweet watermelon accessions apparently contain diversity of the same magnitude as the cow-melons.

  7. Novel and Stress Relevant EST Derived SSR Markers Developed and Validated in Peanut.

    Directory of Open Access Journals (Sweden)

    Tejas C Bosamia

    Full Text Available With the aim to increase the number of functional markers in resource poor crop like cultivated peanut (Arachis hypogaea, large numbers of available expressed sequence tags (ESTs in the public databases, were employed for the development of novel EST derived simple sequence repeat (SSR markers. From 16424 unigenes, 2784 (16.95% SSRs containing unigenes having 3373 SSR motifs were identified. Of these, 2027 (72.81% sequences were annotated and 4124 gene ontology terms were assigned. Among different SSR motif-classes, tri-nucleotide repeats (33.86% were the most abundant followed by di-nucleotide repeats (27.51% while AG/CT (20.7% and AAG/CTT (13.25% were the most abundant repeat-motifs. A total of 2456 EST-SSR novel primer pairs were designed, of which 366 unigenes having relevance to various stresses and other functions, were PCR validated using a set of 11 diverse peanut genotypes. Of these, 340 (92.62% primer pairs yielded clear and scorable PCR products and 39 (10.66% primer pairs exhibited polymorphisms. Overall, the number of alleles per marker ranged from 1-12 with an average of 3.77 and the PIC ranged from 0.028 to 0.375 with an average of 0.325. The identified EST-SSRs not only enriched the existing molecular markers kitty, but would also facilitate the targeted research in marker-trait association for various stresses, inter-specific studies and genetic diversity analysis in peanut.

  8. SSR mining in oil palm EST database: application in oil palm ...

    Indian Academy of Sciences (India)

    Elaeis oleifera. Colombia. 10. Panama. 10. Costa Rica. 10. Subtotal. 30. Palmae Cocoeae Butiinae. Cocos nucifera. Solomon Island. 3. Palmae Areceae. Euterpeinae ..... Ngoot-Chin Ting et al. Ta b le. 6 . Su mmary o f th e tran sferab ility o f. 1. 0. SSR lo ci acro ss sp ecies an d tax a in th e. P almae family. Palm ae. Cocoeae.

  9. With or without articles? A comparison of article-like determiners in Estonian and Finnish

    Directory of Open Access Journals (Sweden)

    Helen Hint

    2017-10-01

    Full Text Available In this paper, we compare the use and functions of definite and indefinite article-like determiners in Estonian and Finnish. Our main aim is to explore whether the factors that explain the choice of particular determiner forms are similar in Estonian and Finnish. We use a picture-sequence based elicitation experiment to collect spoken narratives from adult native speakers of Estonian and Finnish, and apply non-parametric tree and forest models to analyze the data. Our findings indicate that number of mention and animacy are important predictor variables in both languages, but their exact effect is divergent. We also find that in Finnish, case of the determiner NP proves to be an important factor, while in Estonian, syntactic role of the NP explains some aspects of determiner form choice. Nevertheless, the overall usage frequency of determiners is modest in the Estonian and Finnish data, and the process of grammaticalizing articles is only in initial stages in both languages. *** Artikliga, artiklita? Eesti ja soome keele artiklilaadsete määratlejate võrdlus Siinses uurimuses analüüsime võrdlevalt artiklilaadseid definiitseid ja indefiniitseid määratlejaid eesti ja soome keeles. Eelkõige kõrvutame eesti keele definiitset määratlejat see ja soome keele definiitseid määratlejaid se ja tämä ning eesti ja soome indefiniitset määratlejat üks/yks(i. Samuti vaatleme eesti keele possessiivpronoomeni oma ning soome 3sg possessiivsufiksi (-nsa/-nsä, -Vn referentsiaalseid omadusi. Uurimuse põhieesmärgiks on selgitada, millised on peamised määratlejate kasutust mõjutavad keelelised faktorid eesti ja soome keeles ning kas need faktorid on keeliti sarnased või erinevad. Ühtlasi otsime vastust küsimusele, kas eesti ja soome keeles on põhjust rääkida määratlejate grammatisatsioonist artikliteks. Uuritav keelematerjal pärineb pildiseeria põhjal kogutud suulistest narratiividest. Uurimuses osales 20 eesti ja 20 soome keele

  10. Tallinn-Milano-Tallinn / Irina Raud

    Index Scriptorium Estoniae

    Raud, Irina, 1945-

    1992-01-01

    4. arhitektuurinäitus "Salon International de Ĺ Architecture" toimus Milanos (28.09.91-06.10.91). Eestit esindasid arhitektid Andres Alver, Tiit Trummal, Vilen Künnapu, Ain Padrik, Jüri Okas, Marika Lõoke ja Irina Raud

  11. Transverse Kick Analysis of SSR1 Due to Possible Geometrical Variations in Fabrication

    International Nuclear Information System (INIS)

    Yakovlev, V.P.; Awida, M.H.; Berrutti, P.; Gonin, I.V.; Khabiboulline, T.N.

    2012-01-01

    Due to fabrication tolerance, it is expected that some geometrical variations could happen to the SSR1 cavities of Project X, like small shifts in the transverse direction of the beam pipe or the spoke. It is necessary to evaluate the resultant transverse kick due to these geometrical variations, in order to make sure that they are within the limits of the correctors in the solenoids. In this paper, we report the transverse kick values for various fabrications errors and the sensitivity of the beam to these errors. Transverse kick that could happen in SSR1 cavities due to geometrical variations of the fabricated cavities from the designed geometry has been analysed and evaluated. From fabrication experience, three kinds of variations were under investigation concerning the alignment of both the beam pipe and spoke with respect to the beam axis. Simulation study has been carried out implementing these variations in the simulation model. CMM measurements of five fabricated SSR1 cavities were carried out to investigate the amount of physical misalignments of the beam pipe and spoke. Bead-pull measurements were also conducted to evaluate the transverse kick values in the fabricated cavities. Simulation and measurements are relatively in good agreement. Maximum kick in the fabricated cavities is within 154 keV that would induce about 1.12 mrad beam deviation, which could be definitely corrected with the 10 mrad specified correctors of Project X.

  12. Global dimming and urbanization: did stronger negative SSR trends collocate with regions of population growth?

    Directory of Open Access Journals (Sweden)

    A. Imamovic

    2016-03-01

    Full Text Available Global dimming refers to the decrease in surface solar radiation (SSR observed from the 1960s to the 1980s at different measurement sites all around the world. It is under debate whether anthropogenic aerosols emitted from urban areas close to the measurement sites are mainly responsible for the dimming. In order to assess this urbanization impact on SSR, we use spatially explicit population density data of 0.08° resolution to construct population indices (PI at 157 high data quality sites. Our study extends previous population-based studies by incorporating distance-weighting as a simple aerosol diffusion model. We measured urbanization in the surrounding of a site as the PI change from 1960 to 1990 and found no negative correlation with the corresponding SSR trends from 1964 to 1989 for the 92 sites in Europe and Japan. For the 39 sites in China the correlation coefficients are significant at the 5 % level and reach around −0.35, while for the 26 remaining Asian, mostly Russian sites the correlation coefficients reach around −0.55 at the 1 % significance level. Results are similar, when the absolute levels of PIs are taken as an indicator for urbanization. Our findings call into question the existence of an urbanization effect for the sites in Europe and Japan, while such an effect cannot be ruled out for the sites in Asia, especially in Russia.

  13. Confirmation of root-knot nematode resistant gene Rmi1 using SSR markers

    Directory of Open Access Journals (Sweden)

    Musarrat Ramzan

    2017-02-01

    Full Text Available Background: The Root Knot Nematode (RKN is a serious economic threat to various cultivated crops worldwide. It is a devastating pest of soybean and responsible to cause severe yield loss in Pakistan. The cultivation of resistant soybean varieties against this pest is the sustainable strategy to manage the heavy loss and increase yield. There is an utmost need to identify RKN resistant varieties of soybean against cultivated in Pakistan. The presented study is an attempt to identify and confirm the presence of resistant gene Rmi1 in soybean. Method: Molecular studies have been done using Simple Sequence Repeat (SSR marker system to identify resistant soybean varieties against Root Knot Nematode (RKN using fifteen (15 indigenous cultivars and four (4 US cultivars. DNA was isolated, purified, quantified and then used to employ various SSR markers. The amplified product is observed using gel documentation system after electrophoresis. Results: Diagnostic SSR markers Satt-358 and Satt-492 have shown the presence of Rmi1 gene in all resistance carrying genotypes. Satt-358 amplified the fragment of 200 bp and Satt-492 generated 232 bp bands in all resistant genotypes. This study confirmed the Rmi gene locus (G248A-1 in all internationally confirmed resistant including six (6 native varieties. Conclusion: These investigations have identified six (6 resistant cultivars revealing the effective and informative sources that can be utilized in breeding programs for the selection of RKN resistance soybean genotypes in Pakistan.

  14. Alleviation SSR and Low Frequency Power Oscillations in Series Compensated Transmission Line using SVC Supplementary Controllers

    Science.gov (United States)

    Kumar, Sanjiv; Kumar, Narendra

    2017-06-01

    In this work, supplementary sub-synchronous damping controllers (SSDC) are proposed for damping sub-synchronous oscillations in power systems with series compensated transmission lines. Series compensation have extensively been used as effective means of increasing the power transfer capability of a transmission lines and improving transient stability limits of power systems. Series compensation with transmission lines may cause sub-synchronous resonance (SSR). The eigenvalue investigation tool is used to ascertain the existence of SSR. It is shown that the addition of supplementary controller is able to stabilize all unstable modes for T-network model. Eigenvalue investigation and time domain transient simulation of detailed nonlinear system are considered to investigate the performance of the controllers. The efficacies of the suggested supplementary controllers are compared on the IEEE first benchmark model for computer simulations of SSR by means of time domain simulation in Matlab/Simulink environment. Supplementary SSDC are considered in order to compare effectiveness of SSDC during higher loading in alleviating the small signal stability problem.

  15. Molecular characterization of sour orange (Citrus aurantium) accessions and their relatives using SSR and SRAP markers.

    Science.gov (United States)

    Polat, I; Kacar, Y A; Yesiloglu, T; Uzun, A; Tuzcu, O; Gulsen, O; Incesu, M; Kafa, G; Turgutoglu, E; Anil, S

    2012-09-13

    Citrus production with its many varieties is of importance since it provides economically important products for Turkish exports. Sour orange is a rootstock commonly used for propagating the different scion varieties. Knowledge of the genetic diversity of the rootstock accessions would be useful in order to improve citrus breeding programs. We studied genetic relationships and diversity of 51 accessions of sour orange (Citrus aurantium) and their relatives using SSR (simple sequence repeat) and SRAP (sequence-related amplified polymorphism) molecular markers. Twenty-one SRAP primer combinations were tested on these accessions and relatives, producing 167 polymorphic fragments, with a mean of 8.0 and a mean polymorphism information content value of 0.47. Seventeen SSR primers also produced 30 polymorphic fragments, with a mean of 1.4 per primer and a mean polymorphism information content value of 0.39. The unweighted pair-group method with arithmetic average analysis using combined SSR and SRAP data showed a similarity range from 0.71 to 1.00 among the accessions. In the cluster analysis, sour orange relatives were indicated as a separate group from sour orange. 'Macrophylla' and 'Mexican lime' were the accessions most distinct (0.71) from the others. We conclude that genetic diversity in these sour orange accessions is lower and some of them were identical.

  16. Global dimming and urbanization: did stronger negative SSR trends collocate with regions of population growth?

    Science.gov (United States)

    Imamovic, Adel; Tanaka, Katsumasa; Folini, Doris; Wild, Martin

    2016-04-01

    Global dimming refers to the decrease in surface solar radiation (SSR) observed from the 1960s to the 1980s at different measurement sites all around the world. It is under debate whether anthropogenic aerosols emitted from urban areas close to the measurement sites are mainly responsible for the dimming. In order to assess this urbanization impact on SSR, we use spatially explicit population density data of 0.08° resolution to construct population indices (PI) at 157 high data quality sites. Our study extends previous population-based studies by incorporating distance-weighting as a simple aerosol diffusion model. We measured urbanization in the surrounding of a site as the PI change form 1960 to 1990 and found no negative correlation with the corresponding SSR trends from 1964 to 1989 for the 92 sites in Europe and Japan. For the 39 sites in China the correlation coefficients are significant at the 5 % level and reach around -0.35, while for the 26 remaining Asian, mostly Russian sites the correlation coefficients reach around -0.55 at the 1 % significance level. Results are similar, when the absolute levels of PIs are taken as an indicator for urbanization. Our findings call into question the existence of an urbanization effect for the sites in Europe and Japan, while such an effect cannot be ruled out for the sites in Asia, especially in Russia.

  17. Developing new SSR markers from ESTs of pea (Pisum sativum L.).

    Science.gov (United States)

    Gong, Ya-ming; Xu, Sheng-chun; Mao, Wei-hua; Hu, Qi-zan; Zhang, Gu-wen; Ding, Ju; Li, Ya-dan

    2010-09-01

    The development of expressed sequence tags (ESTs) from pea has provided a useful source for mining novel simple sequence repeat (SSR) markers. In the present research, in order to find EST-derived SSR markers, 18 552 pea ESTs from the National Center for Biotechnology Information (NCBI) database were downloaded and assembled into 10 086 unigenes. A total of 586 microsatellites in 530 unigenes were identified, indicating that merely 5.25% of sequences contained SSRs. The most abundant SSRs within pea were tri-nucleotide repeat motifs, and among all the tri-nucleotide repeats, the motif GAA was the most abundant type. In total, 49 SSRs were used for primer design. EST-SSR loci were subsequently screened on 10 widely adapted varieties in China. Of these, nine loci showed polymorphic profiles that revealed two to three alleles per locus. The polymorphism information content value ranged from 0.18 to 0.58 with an average of 0.41. Furthermore, transferable analysis revealed that some of these loci showed transferability to faba bean. Because of their polymorphism and transferability, these nine novel EST-SSRs will be valuable tools for marker-assisted breeding and comparative mapping of pea in the future.

  18. Supervision, mentorship and peer networks: how Estonian early career researchers get (or fail to get support

    Directory of Open Access Journals (Sweden)

    Jaana Eigi

    2018-03-01

    Full Text Available The paper analyses issues related to supervision and support of early career researchers in Estonian academia. We use nine focus groups interviews conducted in 2015 with representatives of social sciences in order to identify early career researchers’ needs with respect to support, frustrations they may experience, and resources they may have for addressing them. Our crucial contribution is the identification of wider support networks of peers and colleagues that may compensate, partially or even fully, for failures of official supervision. On the basis of our analysis we argue that support for early career researchers should take into account the resources they already possess but also recognise the importance of wider academic culture, including funding and employment patterns, and the roles of supervisors and senior researchers in ensuring successful functioning of support networks. Through analysing the conditions for the development of early career researchers – producers of knowledge – our paper contributes to social epistemology understood as analysis of specific forms of social organisation of knowledge production.

  19. Frost related dieback in Estonian energy plantations of willows in relation to fertilisation and pathogenic bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Cambours, M.A.; Nejad, P. [Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Box 7026, 750 07 Uppsala (Sweden); Heinsoo, K. [Institute of Zoology and Botany, Estonian Agricultural University, Riia 181, 51014 Tartu (Estonia); Granhall, U. [Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, 750 07 Uppsala (Sweden)

    2006-03-15

    Two 9-year old Estonian Salix plantations suffering from dieback were studied: one situated on poor mineral soil and divided into fertilised and unfertilised plots (Saare plantation) and another growing on a well-decomposed and nitrogen-rich organic soil, without fertiliser application (Kambja plantation). Bacteria from internal tissues of visually damaged shoots from seven clones were isolated in spring and autumn. The strains were subsequently biochemically characterised and tested for ice nucleation activity and pathogenicity on Salix. Some strains were also analysed with 16S rRNA. High numbers of culturable bacteria were found, belonging mainly to Erwinia, Sphingomonas, Pseudomonas and Xanthomonas spp. Fertilised plots were significantly more colonised by bacteria than unfertilised plots and also more extensively damaged, showing a lower density of living plants after 7 years of culture. More ice nucleation active (INA) strains were found in Saare fertilised plots and at Kambja than in Saare unfertilised plots. Likewise, most pathogenic strains were isolated from Saare fertilised plots and from Kambja. For some of the willow clones studied, dieback appeared to be related to both clonal frost sensitivity and abundance of INA and pathogenic bacteria. The plantations probably suffered from the presence of high amounts of pathogens and from frost related injuries aggravated by INA bacteria. Most probably the fertilisation at Saare and the nitrogen-rich soil at Kambja created a favourable environment for bacterial development and led to high dieback levels after the first harvest. (author)

  20. The very first words of Estonian children: A comparison of two parental report types

    Directory of Open Access Journals (Sweden)

    Astra Schults

    2015-06-01

    Full Text Available Background: Parental recognition has been shown to produce more accurate information about child’s vocabulary compared to remembering. The differences in reporting vocabulary are of interest. Our aims were to determine the extent of vocabulary overlap in the two report types, to identify the word report frequency and the proportions of word categories according to the report type. Methods: The subjects were 219 children (125 boys and 94 girls at the age of 0;8 to 1;4 (M = 10.41, SD = 1.96 who had one to three words reported in the ECDI Infant Form. In this study parents reported children’s current vocabulary in two ways: Estonian version of MacArthur-Bates Communicative Development Inventory: Language and Gestures (henceforth ECDI Infant Form and free recall. Results: On 40% of occasions a word was reported in both ways. Three most frequent words in both report types were aitäh ‘thanks’, nämm-nämm ‘yum-yum’, and emme ‘mommy’. Words belonging to the categories of sound effects and animal sounds as well as social terms had higher average frequency of reports than common nouns regardless of report type. Conclusions: These results may reflect differences in what parents consider to be a word and raise a question of social desirability in parental reports.

  1. Fungal associates of Pyrola rotundifolia, a mixotrophic Ericaceae, from two Estonian boreal forests.

    Science.gov (United States)

    Vincenot, Lucie; Tedersoo, Leho; Richard, Franck; Horcine, Houria; Kõljalg, Urmas; Selosse, Marc-André

    2008-12-01

    Pyrola rotundifolia (Ericaceae, Pyroleae tribe) is an understorey subshrub that was recently demonstrated to receive considerable amount of carbon from its fungal mycorrhizal associates. So far, little is known of the identity of these fungi and the mycorrhizal anatomy in the Pyroleae. Using 140 mycorrhizal root fragments collected from two Estonian boreal forests already studied in the context of mixotrophic Ericaceae in sequence analysis of the ribosomal DNA internal transcribed spacer region, we recovered 71 sequences that corresponded to 45 putative species in 19 fungal genera. The identified fungi were mainly ectomycorrhizal basidiomycetes, including Tomentella, Cortinarius, Russula, Hebeloma, as well as some ectomycorrhizal and/or endophytic ascomycetes. The P. rotundifolia fungal communities of the two forests did not differ significantly in terms of species richness, diversity and nutritional mode. The relatively high diversity retrieved suggests that P. rotundifolia does not have a strict preference for any fungal taxa. Anatomical analyses showed typical arbutoid mycorrhizae, with variable mantle structures, uniseriate Hartig nets and intracellular hyphal coils in the large epidermal cells. Whenever compared, fungal ultrastructure was congruent with the molecular identification. Similarly to other mixotrophic and autotrophic pyroloids in the same forests, P. rotundifolia shares its mycorrhizal fungal associates with surrounding trees that are likely a carbon source for pyroloids.

  2. Symptoms of anxiety and depression in Estonian medical students with sleep problems.

    Science.gov (United States)

    Eller, Triin; Aluoja, Anu; Vasar, Veiko; Veldi, Marlit

    2006-01-01

    High emotional stress in medical students has been observed in many studies. Our aim in this article was to assess the prevalence of symptoms of anxiety and depression among Estonian medical students and to find relationships between sleep complaints and emotional symptoms. The study group consisted of 413 medical students, ages 19-33 years, at the University of Tartu. Each was asked to complete two questionnaires: the Emotional State Questionnaire (EST-Q), containing 28 questions, and the Questionnaire on Sleep and Daytime Habits, with 25 questions. The anxiety and depression subscales from the EST-Q were applied. From the study group, 21.9% students had symptoms of anxiety, and 30.6% had symptoms of depression. The frequency of anxiety and depressive symptoms was higher in females. In regression and multiple regression analysis, we determined which sleep problems were related to emotional symptoms. The associations were different for men and women. In women, anxiety remained significantly related to waking up because of nightmares and feeling tired in the morning; depressive symptoms were related to difficulties in getting to sleep at night, waking up because of nightmares and nocturnal eating habits, daytime sleepiness, and sleepiness during school lessons. In men, significant relations were clear only for depression: difficulties in falling asleep at night before an exam and subjective sleep quality. The study demonstrated that a high percentage of medical students had emotional symptoms. We found that some sleep problems indicated underlying symptoms of anxiety and depression. (c) 2006 Wiley-Liss, Inc.

  3. Trade Union Responses to Transnational Labour Mobility in the Finnish-Estonian Context

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    Rolle Alho

    2013-09-01

    Full Text Available This article examines trade union strategies in relation to labor migration in Estonia and Finland, drawing on face-to-face interviews with trade unionists and official union statements. The study considers the national trade union strategies located in two separate but interconnected localities that represent different approaches to market economy. Previous research suggests that the national industrial relations system is a key factor in explaining unions’ labor migration strategies. Unions operating in liberal market economies are claimed to be more open toward immigration and more inclusive toward immigrants than unions in coordinated markets. This study analyzes the extent to which this theory holds in the context of Estonia and Finland—Finland representing a coordinated market economy and Estonia a liberal market economy. Furthermore, the analysis examines how the emergence of a translocal labor market, resulting from the geographical vicinity and linguistic affinity between Finland and Estonia as well as from free mobility within the EU, is reflected in trade union approaches to labor migration. The study finds that Finnish trade union strategies influence labor mobility, whereas Estonian trade unions remain bystanders in the issue.

  4. Kohtumispaigad: Tallinna kohvikute interjööre = Meeting Places : Interiors of Tallinn Cafes / Kai Lobjakas ; tõlk. Laila Põdra

    Index Scriptorium Estoniae

    Lobjakas, Kai, 1975-

    2003-01-01

    Tallinna kohvikutest aastail 1930-2003. Vaadeldakse kohvikuid Corso/Harju, Feischner/Tallinn, Palace, Kultas/Moskva, Energia, Narva, Pärl, Pegasus, Tuluke, Szolnok, Tuljak, Vigri, Toome, Corrida, Võitlev Sõna, Robert's Coffee, Rossini, Popular. Sisekujundajad: R. Natus, V. Tomassov, K. Herman, B. Linde, J. Siirak, V. Asi, V. Tamm, L. Pärtelpoeg, N. Kultas, P. Aren, H. Karro, G. Nahkur, L. Tarvas, M. Grünberg, A. Murdmaa, M. Tammik, G. Sarv, A. Maasik, A. Nurk, R. Treu, M. Sulengo, R. Tiitus. 22 vaadet, kaart

  5. Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR Marker Resources for Diversity Analysis of Mango (Mangifera indica L.

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    Natalie L. Dillon

    2014-01-01

    Full Text Available In this study, a collection of 24,840 expressed sequence tags (ESTs generated from five mango (Mangifera indica L. cDNA libraries was mined for EST-based simple sequence repeat (SSR markers. Over 1,000 ESTs with SSR motifs were detected from more than 24,000 EST sequences with di- and tri-nucleotide repeat motifs the most abundant. Of these, 25 EST-SSRs in genes involved in plant development, stress response, and fruit color and flavor development pathways were selected, developed into PCR markers and characterized in a population of 32 mango selections including M. indica varieties, and related Mangifera species. Twenty-four of the 25 EST-SSR markers exhibited polymorphisms, identifying a total of 86 alleles with an average of 5.38 alleles per locus, and distinguished between all Mangifera selections. Private alleles were identified for Mangifera species. These newly developed EST-SSR markers enhance the current 11 SSR mango genetic identity panel utilized by the Australian Mango Breeding Program. The current panel has been used to identify progeny and parents for selection and the application of this extended panel will further improve and help to design mango hybridization strategies for increased breeding efficiency.

  6. Genetic diversity of Vietnamese lowland rice germplasms as revealed by SSR markers in relation to seedling vigour under submergence

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    Hien Thi Thu Vu

    2016-01-01

    Full Text Available In the direct-seeding rice cultivation system, seedling vigour is one of the most important traits for stable stand establishment during early seedling stages, particularly under submergence that is caused by temporal flash flood. We studied the genetic diversity in a set of 40 Vietnamese lowland rice varieties using 30 simple sequence repeat (SSR markers covering all rice chromosomes. A total of 111 alleles were detected, with a mean of 3.7 alleles per locus. The number of polymorphic alleles detected by each SSR marker ranged from 2 to 6. The fragment size of a given SSR locus varied between 85 and 650 bp and the frequency of a major allele at each locus ranged from 32.5% to 76.9%. Polymorphism information content value varied from 0.355 to 0.774 with an average of 0.594. The genetic similarity calculated between pairs of rice varieties ranged from 0.03 to 0.97 with an average of 0.27. According to a constructed dendrogram of unweighted pair group method with arithmetic mean based on the SSR marker analysis, the tested rice varieties were clustered into two major groups consisting of five subgroups. Significant correlations existed between the mean genetic similarity and the mean seedling vigour estimated by shoot length under submergence among the tested varieties. Our results suggested usefulness of the SSR marker system to assess genetic diversity in Vietnamese rice germplasms in relation to their seedling vigour under submergence.

  7. Leaf Transcriptome Sequencing for Identifying Genic-SSR Markers and SNP Heterozygosity in Crossbred Mango Variety 'Amrapali' (Mangifera indica L.).

    Science.gov (United States)

    Mahato, Ajay Kumar; Sharma, Nimisha; Singh, Akshay; Srivastav, Manish; Jaiprakash; Singh, Sanjay Kumar; Singh, Anand Kumar; Sharma, Tilak Raj; Singh, Nagendra Kumar

    2016-01-01

    Mango (Mangifera indica L.) is called "king of fruits" due to its sweetness, richness of taste, diversity, large production volume and a variety of end usage. Despite its huge economic importance genomic resources in mango are scarce and genetics of useful horticultural traits are poorly understood. Here we generated deep coverage leaf RNA sequence data for mango parental varieties 'Neelam', 'Dashehari' and their hybrid 'Amrapali' using next generation sequencing technologies. De-novo sequence assembly generated 27,528, 20,771 and 35,182 transcripts for the three genotypes, respectively. The transcripts were further assembled into a non-redundant set of 70,057 unigenes that were used for SSR and SNP identification and annotation. Total 5,465 SSR loci were identified in 4,912 unigenes with 288 type I SSR (n ≥ 20 bp). One hundred type I SSR markers were randomly selected of which 43 yielded PCR amplicons of expected size in the first round of validation and were designated as validated genic-SSR markers. Further, 22,306 SNPs were identified by aligning high quality sequence reads of the three mango varieties to the reference unigene set, revealing significantly enhanced SNP heterozygosity in the hybrid Amrapali. The present study on leaf RNA sequencing of mango varieties and their hybrid provides useful genomic resource for genetic improvement of mango.

  8. Development and characterization of genic SSR markers in Medicago truncatula and their transferability in leguminous and non-leguminous species.

    Science.gov (United States)

    Gupta, Sarika; Prasad, Manoj

    2009-09-01

    Expressed sequence tag (EST)-derived simple sequence repeat (eSSR) markers are important resources for gene discovery and comparative mapping aimed at crop improvement. In this study, we developed eSSR markers for Medicago truncatula and assessed their cross-species transferability. We detected 36,847 non-redundant sequences ("unigenes") from 198,642 M. truncatula EST sequences. Mining of microsatellites from the 36,847 unigene sequences (representing approximately 25.8 Mb) revealed 14,637 eSSRs in 11,750 SSR-containing ESTs, and primer pairs were successfully designed for 4,636 (39.5%). Of the 14 637 eSSRs, 82.6% were mononucleotide repeats and the rest (in descending order of abundance) were tri-, di-, penta-, and tetranucleotide repeats. When less stringent SSR detection criteria were used, the frequency of dinucleotide repeat motifs increased more than twofold, and the frequencies of di- (11%) and trinucleotide motifs (10.6%) were almost equal. This demonstrates that the eSSR frequency and distribution were related to the choice of search criteria. Forty-one randomly selected primer pairs were validated, and their transferability in three leguminous and three non-leguminous species was assessed. The markers showed a high level of transferability in the leguminous (53%-71%) and non-leguminous (33%-44%) species. The validation studies thus demonstrate the utility of the Medicago eSSRs in assessing genomic relationships in both leguminous and non-leguminous species.

  9. EST-SSR marker revealed effective over biochemical and morphological scepticism towards identification of specific turmeric (Curcuma longa L.) cultivars.

    Science.gov (United States)

    Sahoo, Ambika; Jena, Sudipta; Kar, Basudeba; Sahoo, Suprava; Ray, Asit; Singh, Subhashree; Joshi, Raj Kumar; Acharya, Laxmikanta; Nayak, Sanghamitra

    2017-05-01

    Turmeric (Curcuma longa L., family Zingiberaceae) is one of the most economically important plants for its use in food, medicine, and cosmetic industries. Cultivar identification is a major constraint in turmeric, owing to high degree of morphological similarity that in turn, affects its commercialization. The present study addresses this constraint, using EST-SSR marker based, molecular identification of 8 elite cultivars and 88 accessions in turmeric. Fifty EST-SSR primers were screened against eight cultivars of turmeric (Suroma, Roma, Lakadong, Megha, Alleppey Supreme, Kedaram, Pratibha, and Suvarna); out of which 11 primers showed polymorphic banding pattern. The polymorphic information content (PIC) of these primers ranged from 0.13 to 0.48. However, only three SSR loci (CSSR 14, CSSR 15, and CSSR 18) gave reproducible unique banding pattern clearly distinguishing the cultivars 'Lakadong' and 'Suvarna' from other cultivars tested. These three unique SSR markers also proved to be effective in identification of 'Lakadong' cultivars when analysed with 88 accessions of turmeric collected from different agro-climatic regions. Furthermore, two identified cultivars (Lakadong and Suvarna) could also be precisely differentiated when analysed and based on phylogenetic tree, with other 94 genotypes of turmeric. The novel SSR markers can be used for identification and authentication of two commercially important turmeric cultivars 'Lakadong' and 'Suvarna'.

  10. Omaeluloolisus nullindatel / Estonian Life Writing of the 2000s as a Continuity Over the Post-Soviet Period

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    Rutt Hinrikus

    2013-06-01

    Full Text Available A distinctive feature of the (literary culture of the 2000s is a sudden increase in the publication of works of life writing in different genres. Although life writing as a phenomenon has received considerably more critical attention in recent years, it has not yet settled within the literary canon, and theoretical debates concerning its diverse textual practises are still in initial phases. The current article provides an overview of Estonian life writing of the 2000s, viewing the emergence of new forms, themes and perspectives as a continuity within the wider framework of the post-Soviet period and in comparison with some recent developments and theoretical foci of life writing studies in general.The processes of the construction of subjectivity and identity in a number of autobiographical writings published in the first half of the 2000s can be traced back to the emergence of Estonian “memorial culture” in the late 1980s and 1990s, where oral and written testimonial accounts of personal experience focusing on the period of the Soviet occupation played an important role in the process of dismantling the official Soviet discourses of history and contributed to the process of regaining independence. A number of life writing texts by well-known Estonian literary figures published in the mid-2000s employ modes of collective and individual self-conception and self-reflection similar to those prevalent in the texts of life stories. No longer employed for the purposes of implementing socio-political change, these works confirm and consolidate such modes of identification and self-representation within the framework of (literary culture. Another important trend of Estonian life writing of the 2000s involves “textual games”, where lived experience and the construction of fictional worlds are intertwined in a complex and self-conscious manner. Visible most strongly in the work of Tõnu Õnnepalu and Madis Kõiv, this feature of Estonian life

  11. GENETIC DIVERSITY OF RUSSIAN ADVANCED WHEAT CULTIVARS REVEALED WITH SSR MARKERS

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    Potokina E.K.

    2012-08-01

    Full Text Available A quick and reproducible tool for cultivar identification is useful to assess in certified seed production programs and to resolve legal conflicts over the recognition of a seed stock (Mangini et al., 2009. In order to obtain plant breeders’ rights (PBR the new variety has to pass the distinctness-uniformity-stability (DUS criteria in which the candidate breeding lines are compared with existing cultivars on the basis of a series of morphological traits. Although these traits are informative and practical, they exhibit a polygenic control and are subject to environmental influences. Seed storage protein electrophoresis is included in the DUS testing guidelines, but the low level of polymorphism limits the ability to distinguish different genotypes. The resolving power of DNA markers is significantly higher allowing to prove that a new variety is unique from all other varieties that have already been described and that all individuals are as identical as possible.As a part of research project initiated in 2011 we are developing a molecular identification key for 320 wheat cultivars registered in Russian Federation and the Republic of Belarus using SSR markers. A subset of 24 cultivars was randomly selected from the 320 cultivars and screened with 84 genomic SSR primers (Xbarc, Xgwm. At the present stage of project the SSRs with high discriminating ability were further analysed on a set of 96 genotypes. The power of each primer to distinguish among the studied genotypes was estimated by Polymorphism Information Content and the Resolving power. With the selected markers 96 genotypes were easily discriminated. A molecular identification key to distinguish Russian advanced wheat cultivars using SSR-profiling is discussed. We suggest the reproducible fingerprint system for the identification of Russian hexaploid wheat cultivars that could be employed in certified seed production programs to identify sources of seed contamination, and to distinguish

  12. SSR marker variations in Brassica species provide insight into the origin and evolution of Brassica amphidiploids.

    Science.gov (United States)

    Thakur, Ajay Kumar; Singh, Kunwar Harendra; Singh, Lal; Nanjundan, Joghee; Khan, Yasin Jeshima; Singh, Dhiraj

    2018-01-01

    Oilseed Brassica represents an important group of oilseed crops with a long history of evolution and cultivation. To understand the origin and evolution of Brassica amphidiploids, simple sequence repeat (SSR) markers were used to unravel genetic variations in three diploids and three amphidiploid Brassica species of U's triangle along with Eruca sativa as an outlier. Of 124 Brassica-derived SSR loci assayed, 100% cross-transferability was obtained for B. juncea and three subspecies of B. rapa , while lowest cross-transferability (91.93%) was obtained for Eruca sativa . The average % age of cross-transferability across all the seven species was 98.15%. The number of alleles detected at each locus ranged from one to six with an average of 3.41 alleles per primer pair. Neighbor-Joining-based dendrogram divided all the 40 accessions into two main groups composed of B. juncea / B. nigra/B. rapa and B. carinata/B. napus/B. oleracea . C-genome of oilseed Brassica species remained relatively more conserved than A- and B-genome. A- genome present in B. juncea and B. napus seems distinct from each other and hence provides great opportunity for generating diversity through synthesizing amphidiploids from different sources of A- genome. B. juncea had least intra-specific distance indicating narrow genetic base. B. rapa appears to be more primitive species from which other two diploid species might have evolved. The SSR marker set developed in this study will assist in DNA fingerprinting of various Brassica species cultivars, evaluating the genetic diversity in Brassica germplasm, genome mapping and construction of linkage maps, gene tagging and various other genomics-related studies in Brassica species. Further, the evolutionary relationship established among various Brassica species would assist in formulating suitable breeding strategies for widening the genetic base of Brassica amphidiploids by exploiting the genetic diversity present in diploid progenitor gene pools.

  13. Molecular Assortment of Lens Species with Different Adaptations to Drought Conditions Using SSR Markers.

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    Dharmendra Singh

    Full Text Available The success of drought tolerance breeding programs can be enhanced through molecular assortment of germplasm. This study was designed to characterize molecular diversity within and between Lens species with different adaptations to drought stress conditions using SSR markers. Drought stress was applied at seedling stage to study the effects on morpho-physiological traits under controlled condition, where tolerant cultivars and wilds showed 12.8-27.6% and 9.5-23.2% reduction in seed yield per plant respectively. When juxtaposed to field conditions, the tolerant cultivars (PDL-1 and PDL-2 and wild (ILWL-314 and ILWL-436 accessions showed 10.5-26.5% and 7.5%-15.6% reduction in seed yield per plant, respectively under rain-fed conditions. The reductions in seed yield in the two tolerant cultivars and wilds under severe drought condition were 48-49% and 30.5-45.3% respectively. A set of 258 alleles were identified among 278 genotypes using 35 SSR markers. Genetic diversity and polymorphism information contents varied between 0.321-0.854 and 0.299-0.836, with mean value of 0.682 and 0.643, respectively. All the genotypes were clustered into 11 groups based on SSR markers. Tolerant genotypes were grouped in cluster 6 while sensitive ones were mainly grouped into cluster 7. Wild accessions were separated from cultivars on the basis of both population structure and cluster analysis. Cluster analysis has further grouped the wild accessions on the basis of species and sub-species into 5 clusters. Physiological and morphological characters under drought stress were significantly (P = 0.05 different among microsatellite clusters. These findings suggest that drought adaptation is variable among wild and cultivated genotypes. Also, genotypes from contrasting clusters can be selected for hybridization which could help in evolution of better segregants for improving drought tolerance in lentil.

  14. Desenho e validação de iniciadores microssatélites SSR para mamoneira

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    Edna Lôbo Machado

    2013-11-01

    Full Text Available O objetivo deste trabalho foi desenhar, validar e otimizar pares de iniciadores microssatélites SSR para mamoneira. O desenho dos pares de iniciadores foi feito por meio do aplicativo Websat, a partir de sequências depositadas no GenBank do National Center for Biotechnology Information (GenBank/NCBI, e a sua qualidade foi aferida com uso do aplicativo web NetPrimer. Foram utilizadas diferentes concentrações de DNA, cloreto de magnésio, pares de iniciadores, dNTPs e temperatura de anelamento para otimização das condições de PCR. Um total de 30 pares de iniciadores SSR foi desenhado, sintetizado e otimizado. O gel de agarose foi utilizado para detecção dos produtos amplificados, e o gel desnaturante de poliacrilamida, na otimização das condições de PCR e na identificação de polimorfismo. Os pares de iniciadores apresentaram percentagem média de guanina/citosina (GC igual a 47,29% e produtos amplificados com tamanhos entre 128 e 381 pb. Vinte e nove pares de iniciadores SSR (96,7% foram validados, dos quais nove foram polimórficos (23,3%. As concentrações otimizadas para amplificação são: DNA, 25 ng; cloreto de magnésio, 1,2 mmol L-1; iniciadores Forward e Reverse, 0,4 mmol L-1; dNTPs, 0,1 mmol L-1; e temperatura de anelamento, 62 a 64ºC. As ferramentas de bioinformática Websat e Net Primer podem ser utilizadas para desenvolver iniciadores microssatélites de qualidade, para a mamoneira, a partir de sequências depositadas no GenBank/NCBI.

  15. Molecular Assortment of Lens Species with Different Adaptations to Drought Conditions Using SSR Markers

    Science.gov (United States)

    Singh, Dharmendra; Singh, Chandan Kumar; Tomar, Ram Sewak Singh; Taunk, Jyoti; Singh, Ranjeet; Maurya, Sadhana; Chaturvedi, Ashish Kumar; Pal, Madan; Singh, Rajendra; Dubey, Sarawan Kumar

    2016-01-01

    The success of drought tolerance breeding programs can be enhanced through molecular assortment of germplasm. This study was designed to characterize molecular diversity within and between Lens species with different adaptations to drought stress conditions using SSR markers. Drought stress was applied at seedling stage to study the effects on morpho-physiological traits under controlled condition, where tolerant cultivars and wilds showed 12.8–27.6% and 9.5–23.2% reduction in seed yield per plant respectively. When juxtaposed to field conditions, the tolerant cultivars (PDL-1 and PDL-2) and wild (ILWL-314 and ILWL-436) accessions showed 10.5–26.5% and 7.5%–15.6% reduction in seed yield per plant, respectively under rain-fed conditions. The reductions in seed yield in the two tolerant cultivars and wilds under severe drought condition were 48–49% and 30.5–45.3% respectively. A set of 258 alleles were identified among 278 genotypes using 35 SSR markers. Genetic diversity and polymorphism information contents varied between 0.321–0.854 and 0.299–0.836, with mean value of 0.682 and 0.643, respectively. All the genotypes were clustered into 11 groups based on SSR markers. Tolerant genotypes were grouped in cluster 6 while sensitive ones were mainly grouped into cluster 7. Wild accessions were separated from cultivars on the basis of both population structure and cluster analysis. Cluster analysis has further grouped the wild accessions on the basis of species and sub-species into 5 clusters. Physiological and morphological characters under drought stress were significantly (P = 0.05) different among microsatellite clusters. These findings suggest that drought adaptation is variable among wild and cultivated genotypes. Also, genotypes from contrasting clusters can be selected for hybridization which could help in evolution of better segregants for improving drought tolerance in lentil. PMID:26808306

  16. PROBLEMS OF THE EFFICIENCY INCREASING OF TRANSPORTATION BY AIR OF UKRAINIAN SSR (1960-1980

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    Anatoliy Gorban

    2015-11-01

    Full Text Available The article is devoted to the problems of the efficiency increasing of the air transportation. The difficulties of increasing the efficiency of transportation by air in Ukrainian SSR in 1960-1980 were researched, factors that adversely affected the organization of the transport sector were determined and depicted. The article analyzes what caused such difficulties and it was found out that the causes of these difficulties are connected with the organizational problems of air transport of Ukrainian SSR, which negatively affected the operation of the industry. The central aim of the research is to focus on the main problems of air transport of Ukrainian SSR. So, we should say that the transport operation of those years was distributed too unevenly and was dependent on the population density of the territory of the republic. Purpose of the article is to determine, compile and analyze the factors that negatively affected the organization of air transportation of the Ukrainian republic and reduced the efficiency of its operation. Results of the research shows technical, organization and economical deficiency of air transport of Ukrainian SSR which caused the ineffectiveness of this type of transport and determines the nature of such difficulties. Statement of the problem. During the specified period (1960–1980 the air transport had undergone rapid development. Many new airlines were opened, airports were being built and reconstructed, the terms of exploiting of turbojet aircrafts were increased, the speed of planes was increasing. All these facts ensured safe and reliable air connection of all district centers, connected Ukraine with the other Soviet republics and foreign countries by air corridors. Ukrainian Department of Civil Aviation became the biggest regional Department of the Ministry of Civil Aviation of the USSR. But, at the same time the intensity of the increase of cargo and passenger transportation since 1970s led to accumulation of

  17. Use of Simple Sequence Repeat (SSR) markers for DNA fingerprinting and diversity analysis of sugarcane (Saccharum spp.) cultivars resistant and susceptible to red rot

    Science.gov (United States)

    In recent years SSR markers have been used widely for the genetic analysis. The objective of present research was to use SSR markers to develop DNA-based genetic identification and analyze genetic relationship of sugarcane cultivars grown in Pakistan either resistant or susceptible to red rot. Twent...

  18. Molecular breeding in Brassica for salt tolerance: importance of microsatellite (SSR) markers for molecular breeding in Brassica

    Science.gov (United States)

    Kumar, Manu; Choi, Ju-Young; Kumari, Nisha; Pareek, Ashwani; Kim, Seong-Ryong

    2015-01-01

    Salinity is one of the important abiotic factors for any crop management in irrigated as well as rainfed areas, which leads to poor harvests. This yield reduction in salt affected soils can be overcome by improving salt tolerance in crops or by soil reclamation. Salty soils can be reclaimed by leaching the salt or by cultivation of salt tolerance crops. Salt tolerance is a quantitative trait controlled by several genes. Poor knowledge about mechanism of its inheritance makes slow progress in its introgression into target crops. Brassica is known to be a good reclamation crop. Inter and intra specific variation within Brassica species shows potential of molecular breeding to raise salinity tolerant genotypes. Among the various molecular markers, SSR markers are getting high attention, since they are randomly sparsed, highly variable and show co-dominant inheritance. Furthermore, as sequencing techniques are improving and softwares to find SSR markers are being developed, SSR markers technology is also evolving rapidly. Comparative SSR marker studies targeting Arabidopsis thaliana and Brassica species which lie in the same family will further aid in studying the salt tolerance related QTLs and subsequent identification of the “candidate genes” and finding out the origin of important QTLs. Although, there are a few reports on molecular breeding for improving salt tolerance using molecular markers in Brassica species, usage of SSR markers has a big potential to improve salt tolerance in Brassica crops. In order to obtain best harvests, role of SSR marker driven breeding approaches play important role and it has been discussed in this review especially for the introgression of salt tolerance traits in crops. PMID:26388887

  19. Molecular diversity and phylogeny of Triticum-Aegilops species possessing D genome revealed by SSR and ISSR markers

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    Moradkhani Hoda

    2015-12-01

    Full Text Available The aim of this study is investigation the applicability of SSR and ISSR markers in evaluating the genetic relationships in twenty accessions of Aegilops and Triticum species with D genome in different ploidy levels. Totally, 119 bands and 46 alleles were detected using ten primers for ISSR and SSR markers, respectively. Polymorphism Information Content values for all primers ranged from 0.345 to 0.375 with an average of 0.367 for SSR, and varied from 0.29 to 0.44 with the average 0.37 for ISSR marker. Analysis of molecular variance (AMOVA revealed that 81% (ISSR and 84% (SSR of variability was partitioned among individuals within populations. Comparing the genetic diversity of Aegilops and Triticum accessions, based on genetic parameters, shows that genetic variation of Ae. crassa and Ae. tauschii species are higher than other species, especially in terms of Nei’s gene diversity. Cluster analysis, based on both markers, separated total accessions in three groups. However, classification based on SSR marker data was not conformed to classification according to ISSR marker data. Principal co-ordinate analysis (PCoA for SSR and ISSR data showed that, the first two components clarified 53.48% and 49.91% of the total variation, respectively. This analysis (PCoA, also, indicated consistent patterns of genetic relationships for ISSR data sets, however, the grouping of accessions was not completely accorded to their own geographical origins. Consequently, a high level of genetic diversity was revealed from the accessions sampled from different eco-geographical regions of Iran.

  20. The Baltic Klint beneath the central Baltic Sea and its comparison with the North Estonian Klint

    Science.gov (United States)

    Tuuling, Igor; Flodén, Tom

    2016-06-01

    Along its contact with the Baltic Shield, the margin of the East European Platform reveals a well-developed, flooded terraced relief. The most striking and consistent set of escarpments at the contact of the Lower Palaeozoic calcareous and terrigenous rocks, known as the Baltic Klint (BK), extends from northwest of Russia to the Swedish island of Öland. Marine seismic reflection profiling in 1990-2004 revealed the central Baltic Sea Klint (BSK) section in detail and enabled comparison of its geology/geomorphology with a classical klint-section onshore, namely the North Estonian Klint (NEK). The conception of the BK onshore, which is based on the land-sea separating terraced relief in northern Estonia, is not fully applicable beneath the sea. Therefore, we consider that the BSK includes the entire terraced Cambrian outcrop. We suggest the term "Baltic Klint Complex" to include the well-terraced margin of the Ordovician limestone outcrop, which is weakly developed in Estonia. Because of a steady lithological framework of the bedrock layers across the southern slope of the Fennoscandian Shield, the central BSK in the western and the NEK in the eastern part of the Baltic Homocline have largely identical morphologies. The North Estonian Ordovician limestone plateau with the calcareous crest of the BK extends across the central Baltic Sea, whereas morphological changes/variations along the Klint base occur due to the east-westerly lithostratigraphic/thickness changes in the siliciclastic Cambrian sequence. The verge of the NEK, located some 30-50 m above sea level, starts to drop in altitude as its east-westerly course turns to northeast-southwest in western Estonia. Further westwards, the BK shifts gradually into southerly deepening (0.1-0.2°) layers as its crest drops to c. 150 m below sea level (b.s.l.) near Gotska Sandön. This course change is accompanied by a considerable decrease in thickness of the platform sedimentary cover, as below the central Baltic Sea the

  1. Dataset of SSR markers for ISSR-Suppression-PCR to detect genetic variation in Garcinia mangostana L. in Peninsular Malaysia

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    Sri A’jilah Samsir

    2016-09-01

    Full Text Available In this dataset, we present 15 Simple Sequence Repeat (SSR markers with the motifs (ACn, (GAn, and (ACn(AGn using a ISSR-Suppression-PCR technique in order to discriminate Garcinia mangostana from diverse geographical origins in Peninsular Malaysia. A few loci showed differences between 3 and 6 bp in allele size, indicating that there are some polymorphisms between individuals correlating to the number of SSR repeats that may be useful for differentiate of genotypes. Collectively, these data show that the ISSR-Suppression-PCR is a valuable method to illustrate genetic variation of selected G. mangostana in Malaysia.

  2. Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean (Ricinus communis L.

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    Yang Jun-Bo

    2010-12-01

    Full Text Available Abstract Background The castor bean (Ricinus communis L., a monotypic species in the spurge family (Euphorbiaceae, 2n = 20, is an important non-edible oilseed crop widely cultivated in tropical, sub-tropical and temperate countries for its high economic value. Because of the high level of ricinoleic acid (over 85% in its seed oil, the castor bean seed derivatives are often used in aviation oil, lubricants, nylon, dyes, inks, soaps, adhesive and biodiesel. Due to lack of efficient molecular markers, little is known about the population genetic diversity and the genetic relationships among castor bean germplasm. Efficient and robust molecular markers are increasingly needed for breeding and improving varieties in castor bean. The advent of modern genomics has produced large amounts of publicly available DNA sequence data. In particular, expressed sequence tags (ESTs provide valuable resources to develop gene-associated SSR markers. Results In total, 18,928 publicly available non-redundant castor bean EST sequences, representing approximately 17.03 Mb, were evaluated and 7732 SSR sites in 5,122 ESTs were identified by data mining. Castor bean exhibited considerably high frequency of EST-SSRs. We developed and characterized 118 polymorphic EST-SSR markers from 379 primer pairs flanking repeats by screening 24 castor bean samples collected from different countries. A total of 350 alleles were identified from 118 polymorphic SSR loci, ranging from 2-6 per locus (A with an average of 2.97. The EST-SSR markers developed displayed moderate gene diversity (He with an average of 0.41. Genetic relationships among 24 germplasms were investigated using the genotypes of 350 alleles, showing geographic pattern of genotypes across genetic diversity centers of castor bean. Conclusion Castor bean EST sequences exhibited considerably high frequency of SSR sites, and were rich resources for developing EST-SSR markers. These EST-SSR markers would be particularly

  3. Personality traits and eating habits in a large sample of Estonians.

    Science.gov (United States)

    Mõttus, René; Realo, Anu; Allik, Jüri; Deary, Ian J; Esko, Tõnu; Metspalu, Andres

    2012-11-01

    Diet has health consequences, which makes knowing the psychological correlates of dietary habits important. Associations between dietary habits and personality traits were examined in a large sample of Estonians (N = 1,691) aged between 18 and 89 years. Dietary habits were measured using 11 items, which grouped into two factors reflecting (a) health aware and (b) traditional dietary patterns. The health aware diet factor was defined by eating more cereal and dairy products, fish, vegetables and fruits. The traditional diet factor was defined by eating more potatoes, meat and meat products, and bread. Personality was assessed by participants themselves and by people who knew them well. The questionnaire used was the NEO Personality Inventory-3, which measures the Five-Factor Model personality broad traits of Neuroticism, Extraversion, Openness, Agreeableness, and Conscientiousness, along with six facets for each trait. Gender, age and educational level were controlled for. Higher scores on the health aware diet factor were associated with lower Neuroticism, and higher Extraversion, Openness and Conscientiousness (effect sizes were modest: r = .11 to 0.17 in self-ratings, and r = .08 to 0.11 in informant-ratings, ps < 0.01 or lower). Higher scores on the traditional diet factor were related to lower levels of Openness (r = -0.14 and -0.13, p < .001, self- and informant-ratings, respectively). Endorsement of healthy and avoidance of traditional dietary items are associated with people's personality trait levels, especially higher Openness. The results may inform dietary interventions with respect to possible barriers to diet change.

  4. Characterization of the vaginal micro- and mycobiome in asymptomatic reproductive-age Estonian women.

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    Tiina Drell

    Full Text Available The application of high-throughput sequencing methods has raised doubt in the concept of the uniform healthy vaginal microbiota consisting predominantly of lactobacilli by revealing the existence of more variable bacterial community composition. As this needs to be analyzed more extensively and there is little straightforward data regarding the vaginal mycobiome of asymptomatic women we aimed to define bacterial and fungal communities in vaginal samples from 494 asymptomatic, reproductive-age Estonian women. The composition of the vaginal microbiota was determined by amplifying bacterial 16S rRNA and fungal internal transcribed spacer-1 (ITS-1 regions and subsequently sequencing them using 454 Life Sciences pyrosequencing. We delineated five major bacterial community groups with distinctive diversity and species composition. Lactobacilli were among the most abundant bacteria in all groups, but also members of genus Gardnerella had high relative abundance in some of the groups. Microbial diversity increased with higher vaginal pH values, and was also higher when a malodorous discharge was present, indicating that some of the women who consider themselves healthy may potentially have asymptomatic bacterial vaginosis (BV. Our study is the first of its kind to analyze the mycobiome that colonizes the healthy vaginal environment using barcoded pyrosequencing technology. We observed 196 fungal operational taxonomic units (OTUs, including 16 OTUs of Candida spp., which is more diverse than previously recognized. However, assessing true fungal diversity was complicated because of the problems regarding the possible air-borne contamination and bioinformatics used for identification of fungal taxons as significant proportion of fungal sequences were assigned to unspecified OTUs.

  5. Non-cancer morbidity among Estonian Chernobyl cleanup workers: a register-based cohort study.

    Science.gov (United States)

    Rahu, Kaja; Bromet, Evelyn J; Hakulinen, Timo; Auvinen, Anssi; Uusküla, Anneli; Rahu, Mati

    2014-05-14

    To examine non-cancer morbidity in the Estonian Chernobyl cleanup workers cohort compared with the population sample with special attention to radiation-related diseases and mental health disorders. Register-based cohort study. Estonia. An exposed cohort of 3680 men (cleanup workers) and an unexposed cohort of 7631 men (population sample) were followed from 2004 to 2012 through the Population Registry and Health Insurance Fund database. Morbidity in the exposed cohort compared with the unexposed controls was estimated in terms of rate ratio (RR) with 95% CIs using Poisson regression models. Elevated morbidity in the exposed cohort was found for diseases of the nervous system, digestive system, musculoskeletal system, ischaemic heart disease and for external causes. The most salient excess risk was observed for thyroid diseases (RR=1.69; 95% CI 1.38 to 2.07), intentional self-harm (RR=1.47; 95% CI 1.04 to 2.09) and selected alcohol-related diagnoses (RR=1.25; 95% CI 1.12 to 1.39). No increase in morbidity for stress reactions, depression, headaches or sleep disorders was detected. No obvious excess morbidity consistent with biological effects of radiation was seen in the exposed cohort, with the possible exception of benign thyroid diseases. Increased alcohol-induced morbidity may reflect alcohol abuse, and could underlie some of the higher morbidity rates. Mental disorders in the exposed cohort were probably under-reported. The future challenge will be to study mental and physical comorbidities in the Chernobyl cleanup workers cohort. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

  6. Corporate Governance from the Perspective of Stakeholder Theory and in Light of Perceptions among Estonian Owners and Managers of Relations with Stakeholders / Mari Kooskora

    Index Scriptorium Estoniae

    Kooskora, Mari, 1969-

    2006-01-01

    Äriühingute valitsemise kontseptsioon ja teoreetiline taust; Eesti omanike ja tippjuhtide ootused suhetes erinevate huvigruppidega ning äriühingute valitsemine huvigruppide teooriast lähtudes. Skeem: The stakeholder model. Tabelid: Contractual and community stakeholders; Overview of how perceptions of the main stakeholder groups among Estonian business leaders between 1995-2004 have changed

  7. Economic modelling of the capture-transport-sink scenario of industrial CO2 emissions: The Estonian-Latvian cross-border case study

    NARCIS (Netherlands)

    Shogenova, A.; Shogenov, K.; Pomeranceva, R.; Nulle, I.; Neele, F.; Hendriks, C.

    2011-01-01

    Industrial CO2 emissions and opportunities for CO2 geological storage in the Baltic Region were studied within the EU GeoCapacity project supported by the European Union Framework Programme 6. Estonia produces the largest amounts of CO2 emissions in the region, due to the combustion of Estonian oil

  8. “... if there’s a party, then there’s definitely alcohol”. Construction of partying practices and abstinence in Estonian youth forums

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    Parder Mari-Liisa

    2015-12-01

    Full Text Available AIMS – Adolescents’ abstinence from alcohol has not been much researched in terms of providing suggestions for prevention strategies. This study aims to fill that gap by offering a practice theory-inspired analysis of how the unwritten rules of partying practices are communicated between posters of Estonian youth forums.

  9. Attitudes of Academic Staff towards Their Own Work and towards External Evaluation, from the Perspective of Self-Determination Theory: Estonian Case

    Science.gov (United States)

    Seema, Riin; Udam, Maiki; Mattisen, Heli

    2016-01-01

    The purpose of this study was to ascertain the attitudes of academic staff towards their own work as well as towards external evaluations. The study was based on (1) an analysis of assessment reports of institutional accreditations conducted by the Estonian Quality Agency for Higher and Vocational Education and (2) self-determination theory on…

  10. Euroopa-ihalusest taasiseseisvusperioodi autobiograafiates / European Identifications in Post-Soviet Estonian Life Writing

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    Leena Kurvet-Käosaar

    2016-12-01

    Full Text Available Teesid: Artikkel käsitleb Euroopat kui identiteedi ja minaduse sõlmpunkti ja enesevahenduse mõõdet kolmes omaelulookirjutuslikus teoses, mille autoritel on väljapaistev positsioon eesti (kirjanduskultuuris: Jaan Krossi „Kallid kaasteelised“ (2003a, 2008, Jaan Kaplinski „Isale“ (2003 ja Tõnu Õnnepalu „Flandria päevik“ (2007. Artikkel keskendub sellele, kuidas küsimused suhestumise trajektooridest Euroopa mäluruumiga haakuvad Krossi, Kaplinski ja Õnnepalu teostes esiletuleva enesemääratlusliku raamiga, neist lähtuvate vastastikuse kõnetuse võimaluste ja oma aegruumi tunnetuse pidepunktidega. Kuigi tegemist on eesti keeles ilmunud ning eesti kultuuriruumi lugejale suunatud teostega, seostuvad neis väljajoonistuvad enesemääratluse teljed viimastel aastakümnetel hoogustunud laiemate aruteludega ühtse Euroopa mäluraami ning identiteedi võimalikkusest ja selle toimimise tingimustest.   In recent years, the question of the possibility of a shared frame of memory and identity in Europe, its desired manifestations and practices for attaining it as well as its obstacles and limitations to it have gained prominence in scholarly debates in a number of disciplines. In terms of the division between the East and West of Europe, these discussions take as their starting point the collapse of the Soviet system in 1989 as well as the European Union enlargement in 2004. Far from uniform, the exceedingly complex and contrasting ranges of arguments have put forward a varied palette of perspectives and suggestions about possible and desirable implications of Europe in different socio-political and cultural configurations. Taking these debates as my starting point, the current article offers an analysis of three life writing works by contemporary Estonian authors and intellectuals, Kallid kaasteelised (Dear Fellow Travellers, 2003, 2008 by Jaan Kross, Isale (To My Father, 2003 by Jaan Kaplinski and Flandria päevik (The Flemish Diary

  11. Omaeluloolisus eesti teatris: Merle Karusoo lavastustest. Life Narratives and Estonian Theatre: The Productions of Merle Karusoo

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    Piret Kruuspere

    2012-04-01

    Full Text Available Any consideration of Estonian theatre from the point of view of biographical theatre needs to include the work of playwright and director Merle Karusoo. Productions based on various life narratives (diaries, letters, biographical interviews form the core of her work that can be defined as biographical or memory theatre. Her work has also been viewed within the context of community theatre or political theatre; Karusoo has herself referred to her work as sociological theatre. Life narratives have functioned in Karusoo’s productions as the basis for restoring oppressed or denied collective discourses of memory. Her productions emerged within the framework of the more general process of restoration of historical heritage and the rehabilitation of collective memory at the end of the 1980s and the beginning of the 1990s. Life story can be viewed as the essence of Merle Karusoo’s theatre. The personal in the life story in the production activates the emotional memory of the audience; for older generations such theatre facilitates a legitimisation of remembering one’s life story in entirety, and for younger generations it functions as a vehicle of collective, historical and national memory. The current article outlines the main stages of Karusoo’s biographical theatre, highlights major productions of each stage and provides an overview of their reception. Karusoo’s theatre dates back to 1980s. Productions based on life stories of the generations born in 1950s and 1960s, Meie elulood (Our Biographies and Kui ruumid on täis ... (Full Rooms both in 1982, mediated fragments of life stories of 16 drama students, focusing on the processes of self-conception and -reflection of young persons. In the context of the Soviet regime that exerted firm ideological control over the private lives of its citizens, Karusoo’s productions struck an especially powerful and unusual chord. Karusoo’s biographical theatre has gathered momentum and assumed a more

  12. Genetic Characterization of Green Bean (Phaseolus vulgaris L.) Accessions from Turkey with SCAR and SSR Markers.

    Science.gov (United States)

    Madakbaş, Seher Yıldız; Sarıkamış, Gölge; Başak, Hakan; Karadavut, Ufuk; Özmen, Canan Yüksel; Daşçı, Mete Gürhan; Çayan, Selin

    2016-08-01

    Characterization, conservation, and utilization of genetic resources is essential for the sustainability in agriculture. Plant genetic resources are important for breeding efforts designed for the generation of new cultivars or for the improvement of existing ones. Green bean has been cultivated extensively in Turkey giving rise to local accessions through selection over time and adaptation to various environmental conditions. The objective of the present study was to determine the genetic relationships of green bean accessions collected from Kırşehir Province of Turkey, located at the central Anatolia. Within a population of 275 green bean accessions, 50 accessions were selected on the basis of morphological observations for further evaluation with SSR and STS/SCAR markers together with 4 reference cultivars of Andean and Mesoamerican origin. SSR markers selected on the basis of high polymorphism information content revealed the genetic relatedness of selected green bean accessions. STS/SCAR markers associated with bean anthracnose, common bacterial blight, white mold, halo blight, and phaseolin protein demonstrated the inheritance of resistance traits of local accessions at the selected loci. These findings may help better utilize genetic resources and furthermore are expected to facilitate forthcoming breeding studies for the generation of novel cultivars well adapted to the region.

  13. Molecular genetic diversity of Punica granatum L. (pomegranate) as revealed by microsatellite DNA markers (SSR).

    Science.gov (United States)

    Hasnaoui, Nejib; Buonamici, Anna; Sebastiani, Federico; Mars, Messaoud; Zhang, Dapeng; Vendramin, Giovanni G

    2012-02-01

    Pomegranate (Punica granatum L.) is one of the oldest known edible fruits and more and more it arouse interest of scientific community given its numerous biological activities. However, information about its genetic resources and characterization using reliable molecular markers are still scarce. In the present study, we report the development of 4 new polymorphic SSR markers. They have been used in addition to 11 SSRs previously published to investigate molecular diversity of 33 P. granatum ecotypes. Based on the multi-locus profiles, twenty-two distinctive genotypes were identified. Globally, quite low genetic diversity has been revealed, as measured by allele richness (2.83 per locus) and heterozygosity (He=0.245; Ho=0.243), reflecting the narrow genetic background of the plant material. Four synonymous groups could be detected involving 15 accessions. Results of ordination and cluster analysis suggested that almost all the Tunisian cultivars share similar genetic background, and are likely derived from a small number of introductions in ancient times. Results issued from this study provide essential information to project a pomegranate core-collection without plant material duplication and for sustainable management of pomegranate landraces at national and international level. Furthermore, these SSR markers are powerful tool for marker assisted selection (MAS) program and for QTL studies. Copyright © 2011 Elsevier B.V. All rights reserved.

  14. Development of an SSR-based identification key for Tunisian local almonds

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    Hassouna Gouta

    2012-04-01

    Full Text Available Ten simple sequence repeat (SSR loci were used to study polymorphism in 54 almond genotypes. All genotypes used in this study originated from almond-growing areas in Tunisia with different climatic conditions ranging from the sub-humid to the arid and are preserved in the national collection at Sidi Bouzid. Using ten SSR, 130 alleles and 250 genotypes were revealed. In order to develop an identification key for each accession, the data were analysed separately for each microsatellite marker. The most polymorphic microsatellite (CPDCT042 was used as a first marker. Two microsatellite loci (CPDCT042 and CPDCT025 were sufficient to discriminate among all accessions studied. Neighbour-joining clustering and principal coordinate analysis were performed to arrange the genotypes according to their genetic relationships and origin. The results are discussed in the context of almond collection management, conformity checks, identification of homonyms, and screening of the local almond germplasm. Furthermore, this microsatellite-based key is a first step toward a marker-assisted identification almond database.

  15. Species discrimination, population structure and linkage disequilibrium in Eucalyptus camaldulensis and Eucalyptus tereticornis using SSR markers.

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    Shanmugapriya Arumugasundaram

    Full Text Available Eucalyptus camaldulensis and E. tereticornis are closely related species commonly cultivated for pulp wood in many tropical countries including India. Understanding the genetic structure and linkage disequilibrium (LD existing in these species is essential for the improvement of industrially important traits. Our goal was to evaluate the use of simple sequence repeat (SSR loci for species discrimination, population structure and LD analysis in these species. Investigations were carried out with the most common alleles in 93 accessions belonging to these two species using 62 SSR markers through cross amplification. The polymorphic information content (PIC ranged from 0.44 to 0.93 and 0.36 to 0.93 in E. camaldulensis and E. tereticornis respectively. A clear delineation between the two species was evident based on the analysis of population structure and species-specific alleles. Significant genotypic LD was found in E. camaldulensis, wherein out of 135 significant pairs, 17 pairs showed r(2≥0.1. Similarly, in E. tereticornis, out of 136 significant pairs, 18 pairs showed r(2≥0.1. The extent of LD decayed rapidly showing the significance of association analyses in eucalypts with higher resolution markers. The availability of whole genome sequence for E. grandis and the synteny and co-linearity in the genome of eucalypts, will allow genome-wide genotyping using microsatellites or single nucleotide polymorphims.

  16. SSR characterization of Oryza glumaepatula populations from the Brazilian Amazon and Cerrado biomes.

    Science.gov (United States)

    Abreu, Aluana Gonçalves; Rosa, Thalita Marra; Borba, Tereza Cristina de Oliveira; Vianello, Rosana Pereira; Rangel, Paulo Hideo Nakano; Brondani, Claudio

    2015-08-01

    The level and distribution of the genetic variability in 18 natural populations of Oryza glumaepatula that were collected from two Brazilian states were estimated using a set of 23 highly informative SSR markers. Samples comprising 78 and 117 individuals from populations of the states of Tocantins and Roraima, respectively, were evaluated in order to integrate and support previous studies that were carried out with populations of O. glumaepatula from Brazil. A total of 189 alleles were identified with an average of 8.22 alleles per locus. The 11 populations from Roraima presented, in combination, a higher genetic diversity (HE = 0.245) compared with that of the seven populations from Tocantins (HE = 0.212). All of the populations showed high and significant inbreeding values (mean f = 0.59); however, the mean was higher in Tocantins populations, indicating a higher gene flow in Roraima populations. The overall coefficient of genetic differentiation (FST) among the populations was high and significant (0.59) and was higher in Tocantins due to the isolation of each population, in contrast to Roraima, where gene flow occurred more frequently. The SSR panel used in this work resulted to be informative (polymorphism information content = 0.201) for assessing genetic structure in O. glumaepatula populations.

  17. De novo transcriptomic analysis of cowpea (Vigna unguiculata L. Walp.) for genic SSR marker development.

    Science.gov (United States)

    Chen, Honglin; Wang, Lixia; Liu, Xiaoyan; Hu, Liangliang; Wang, Suhua; Cheng, Xuzhen

    2017-07-11

    Cowpea [Vigna unguiculata (L.) Walp.] is one of the most important legumes in tropical and semi-arid regions. However, there is relatively little genomic information available for genetic research on and breeding of cowpea. The objectives of this study were to analyse the cowpea transcriptome and develop genic molecular markers for future genetic studies of this genus. Approximately 54 million high-quality cDNA sequence reads were obtained from cowpea based on Illumina paired-end sequencing technology and were de novo assembled to generate 47,899 unigenes with an N50 length of 1534 bp. Sequence similarity analysis revealed 36,289 unigenes (75.8%) with significant similarity to known proteins in the non-redundant (Nr) protein database, 23,471 unigenes (49.0%) with BLAST hits in the Swiss-Prot database, and 20,654 unigenes (43.1%) with high similarity in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Further analysis identified 5560 simple sequence repeats (SSRs) as potential genic molecular markers. Validating a random set of 500 SSR markers yielded 54 polymorphic markers among 32 cowpea accessions. This transcriptomic analysis of cowpea provided a valuable set of genomic data for characterizing genes with important agronomic traits in Vigna unguiculata and a new set of genic SSR markers for further genetic studies and breeding in cowpea and related Vigna species.

  18. Linkage disequilibrium and population structure in Fragaria chiloensis revealed by SSR markers transferred from commercial strawberry

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    Felipe Alberto Oñate

    2017-11-01

    Full Text Available The Chilean strawberry [Fragaria chiloensis (L. Mill.] is the maternal progenitor of the commercial strawberry (Fragaria ´ ananassa Duch., which is characterized by fruits with high organoleptic quality and is well-suited to areas where drought and salinity represent a constraint on crop growth and productivity. We examined the patterns of linkage disequilibrium, genetic diversity and population structure among 54 accessions of F. chiloensis to understand the genetic basis of this species. We used a core microsatellite marker set (n = 95 from a consensus linkage map of strawberry. A transferability rate of 82.1% (78/95 was found, and 38 markers were selected for this study. The SSR primers produced a total of 259 alleles, which varied between 112 and 342 bp. Lower genetic diversity at the species level (HE = 0.17, Shannon’s index = 0.28 was found compared to previous studies of this species. No climatic region pattern for SSR diversity was observed. Structure analysis suggests that the accessions are grouped into three significantly differentiated clusters. Pairwise estimates of φST indicated a low degree of differentiation between the three genetic groups (φST = 0.023 to 0.06. These groups are in concordance with potential glacial refugia in the region, with many accessions being an admixture of them.

  19. Development of simple sequence repeat (SSR) markers of sesame (Sesamum indicum) from a genome survey.

    Science.gov (United States)

    Wei, Xin; Wang, Linhai; Zhang, Yanxin; Qi, Xiaoqiong; Wang, Xiaoling; Ding, Xia; Zhang, Jing; Zhang, Xiurong

    2014-04-22

    Sesame (Sesamum indicum), an important oil crop, is widely grown in tropical and subtropical regions. It provides part of the daily edible oil allowance for almost half of the world's population. A limited number of co-dominant markers has been developed and applied in sesame genetic diversity and germplasm identity studies. Here we report for the first time a whole genome survey used to develop simple sequence repeat (SSR) markers and to detect the genetic diversity of sesame germplasm. From the initial assembled sesame genome, 23,438 SSRs (≥5 repeats) were identified. The most common repeat motif was dinucleotide with a frequency of 84.24%, followed by 13.53% trinucleotide, 1.65% tetranucleotide, 0.3% pentanucleotide and 0.28% hexanucleotide motifs. From 1500 designed and synthesised primer pairs, 218 polymorphic SSRs were developed and used to screen 31 sesame accessions that from 12 countries. STRUCTURE and phylogenetic analyses indicated that all sesame accessions could be divided into two groups: one mainly from China and another from other countries. Cluster analysis classified Chinese major sesame varieties into three groups. These novel SSR markers are a useful tool for genetic linkage map construction, genetic diversity detection, and marker-assisted selective sesame breeding.

  20. Genomic validation of PB 260 clone of rubber (Hevea brasiliensis) at Cikumpay Plantation by SSR marker

    Science.gov (United States)

    Royani, J. I.; Safarrida, A.; Rachmawati, I.; Khairiyah, H.; Mustika, I. P.; Suyono, A.; Rudiyana, Y.; Kubil; Nurjaya; Arianto, A.

    2017-05-01

    Rubber from Hevea brasiliensis is the only commercial natural rubber in the world. Propagation of rubber trees usually done by grafting and seed germination. BPPT had been producing rubber tree by in vitro technique with embryo somatic methods. Validation of mother plant for in vitro propagation is important to compare between mother plant and propagated plants. The aim for this research was to validation of PB 260 clone that planted at Cikumpay Plantation by SSR marker. Sampling of 10 rubber leaves were done at Cikumpay Plantation based on GPS position from the area of PB 260 clone. Rubber leaves were isolated with CTAB modification method to obtained DNA. Four of SSR primers from rubber, i.e.: hmac 4, hmac 5, hmct 1, and hmct 5, were used as primers to amplification of rubber DNA. The result showed that no band that different from 10 rubber of PB 260 clone at Cikumpay Plantation. This research will continue to compare genomic validation between mother plant and propagated plants that had been produced from BPPT.

  1. Genetic diversity of the Bambara groundnut (Vigna subterranea (L.) Verdc.) as assessed by SSR markers.

    Science.gov (United States)

    Somta, P; Chankaew, S; Rungnoi, O; Srinives, P

    2011-11-01

    Bambara groundnut ( Vigna subterranea (L.) Verdc.) is an important African legume crop. In this study, a collection consisting of 240 accessions was analyzed using 22 simple sequence repeat (SSR) markers. In total, 166 alleles were detected, with a mean of 7.59 alleles per locus. Allelic and gene diversities were higher in the west African and Cameroon/Nigeria regions with 6.68 and 6.18 alleles per locus, and 0.601 and 0.571, respectively. The genetic distance showed high similarity between west African and Cameroon/Nigeria accessions. Principal coordinate analyses and neighbor-joining analysis consistently revealed that the majority of west African accessions were grouped with Cameroon/Nigeria accessions, but they were differentiated from east African, central African, and southeast Asian accessions. Population structure analysis showed that two subpopulations existed, and most of the east African accessions were restricted to one subpopulation with some Cameroon/Nigeria accessions, whereas most of the west African accessions were associated with most of the Cameroon/Nigeria accessions in the other subpopulation. Comparison with SSR analysis of other Vigna cultigens, i.e., cultivated azuki bean ( Vigna angularis ) and mungbean ( Vigna radiata ), reveals that the mean gene diversity of Bambara groundnut was lower than azuki bean but higher than mungbean.

  2. Fostering eGovernment as State Social Responsibility (SSR: Case Study of an Australian City Council

    Directory of Open Access Journals (Sweden)

    Sinara Rao Karna

    2012-12-01

    Full Text Available           Democracies around the world now face Citizen-apathy. This is a concern now more than ever faced by countries around the globe. eGovernment is undoubtedly a platform to deliberate and enable citizens regain confidence and faith in democratic  processes. Citizens now seek Verifiable, Open, Transparent, Empathetic, Responsive and Sensitive Electronic Democracy and Government (VOTERS EDG, Karna, 2012. Similar to corporate world, there are voices stressing on govenments for the need to understand the stakeholders, their involvement, relationships and responsibilities of a state in eGovernance. Citizens everywhere now demand Verifiable, Open, Transparent, Empathetic, Responsive and Sensitive Electronically Democratic Government as a State Social Responsibity (SSR. Peoples movements and outbursts against authorities with the help of Word of Mouse (Karna, 2012 have established that transparent and open governance is the need of the hour. This paper presents findings of the study conducted in an Australian City Council for preparing the city council for ‘City e-readiness’ to initiate e-Government activities. We propose the idea of ‘Centrality of Citizens’ in context of eGovernment. We further build upon the original concept of deeming eGovernment as ‘State Social Responsibility’ (SSR (Karna, 2010, by governments at all levels.  

  3. Making a Mark—Time Changing Politics from Estonia: An Alternative Idea for the British, Bulgarian and Estonian EU Presidency

    Directory of Open Access Journals (Sweden)

    Fischer Dirk-Hinnerk

    2016-02-01

    Full Text Available Estonia is famous among politicians for its e-Governance policies. One core ingredient for this progress is the abolishment of outdated technologies and standards as this kind of legacy policy slows down the innovation process. This short paper adapts the Estonian approach towards legacy policy and proposes a political symbol initiated by the EU Presidency Trio of the United Kingdom, Bulgaria and Estonia. The entire topic was inspired by the motivation of the Estonian government to use the presidency to make a mark for Estonia. The twice-yearly occurring process of introducing and disabling the summertime, is not only annoying but has also almost exclusively negative impacts. Meanwhile, the summertime itself comes with many aspects of questionable impact and even opposing empirical results, but it also has some generally agreed upon positive influences. This paper takes side against the process of changing times, but does not take position for one of the possible solutions. This short article shall only inform and show the possibilities in regard to the summer time, the EU presidency and the political symbolism of celebrating the centenary of the First World War.

  4. Diversity of Helicobacter pylori genotypes among Estonian and Russian patients with perforated peptic ulcer, living in Southern Estonia.

    Science.gov (United States)

    Sillakivi, T; Aro, H; Ustav, M; Peetsalu, M; Peetsalu, A; Mikelsaar, M

    2001-02-05

    To compare the genomic variation of Helicobacter pylori in samples obtained from patients with perforated peptic ulcer, living in the same area of Estonia but belonging to different nationalities, 50 non-consecutive patients (32 Estonians and 18 Russians) admitted in the Tartu University Hospital in 1997-1999 were studied. Gastric samples of antral mucosa were obtained during operation and analysed histologically and with PCR for detection of different genotypes of H. pylori (cagA and vacA s and m subtypes). Among the 50 perforated peptic ulcer patients with histologically proven H. pylori colonisation no sample of gastric mucosa showed the s1b subtype of the vacA gene. The perforated peptic ulcer patients were mainly infected with cagA (82%) and s1 (98%) genotypes of H. pylori. The distribution of s1a/m1, s1a/m2 and s2/m2 subtypes of vacA genes was statistically different in Estonian and Russian patients (Ppeptic ulcer from Southern Estonia.

  5. Isamaalaulud ja okupatsioonirežiim – nostalgia, utoopia ja reaalsus. Estonian Patriotic Songs and the Occupational Regime – Nostalgia, Utopia and Reality

    Directory of Open Access Journals (Sweden)

    Kanni Labi

    2012-04-01

    Full Text Available Estonian knowledge of history emanates from the fact that constituting an independent nation has for the most part been nothing but a utopia, and was a reality for a relatively short time. When it comes to selfidentification though, the expression ’a singing people’ is often used by the Estonians to describe themselves. Nostalgia for freedom is reflected in the Estonian culture of almost all periods and is carried on by the singing tradition, where patriotic songs emerge independent of different musical tastes within a particular generation and form an important part of a common Estonian repertoire. Patriotic songs occupy a central place in several fields of Estonian culture: besides music culture also in popular culture,and literary history – the works of poetry which have gained the most popularity are those which when put to music have been the most widely spread among the people. The main part of the most popular Estonian patriotic songs are choral songs from the national awakening at the end of the 19th century. Despite the national programmes aiming to wipe out ‘bourgeois nationalism’, they were sung at the song festivals in the Soviet era and were published in song books, expressing the people’s nostalgia for freedom lost. After the end of the Second World War, there was an attempt at launching a kind of patriotic new creation, where patriotism was merged with Soviet pathos; the aim was to show that the people’s utopia was in fact communism, but not a single one of those songs made it into the people’s common repertoire. Only the patriotic songs composed in the 1980s during the so-called new national awakening reached a popularity comparable to that of the old songs. The discourse on ’Estonianness’ and the shaping of a matching repertoire under imperial Russian rule took place under very different circumstances than its preservation and development in the second half of the 20th century under Soviet occupation, but

  6. [Nordosteuropa als Geschichtsregion : Beiträge des III. Internationalen Symposiums zur Deutschen Kultur und Geschichte im Europäischen Nordosten vom 20. - 22. September 2001 in Tallinn (Estland)] / Janet Laidla

    Index Scriptorium Estoniae

    Laidla, Janet, 1982-

    2008-01-01

    Arvustus: Nordosteuropa als Geschichtsregion : Beiträge des III. Internationalen Symposiums zur Deutschen Kultur und Geschichte im Europäischen Nordosten vom 20. - 22. September 2001 in Tallinn (Estland). (Helsinki : Aue-Stiftung ; Lübeck : Scmidt-Römhild, 2006)

  7. The Futures of Adult Educator(s): Agency, Identity and Ethos. Joint Conference Proceedings of the 2nd ESREA/ReNAdET Meeting and the 4th TQF Seminar (Tallinn, Estonia, November 9-11, 2011)

    Science.gov (United States)

    Heikkinen, Anja, Ed.; Jogi, Larissa, Ed.; Jutte, Wolfgang, Ed.; Zarifis, Georgios K., Ed.

    2012-01-01

    This edited volume contains the papers presented in the 2nd ESREA|ReNAdet meeting that was jointly organised with the VET & CULTURE Network in the University of Tallinn (Estonia), 9-11 November 2011. The papers that appear in the volume discuss the future (or the futures) of adult educators in respect to issues of developing their identities…

  8. [Nordosteuropa als Geschichtsregion. Beiträge des III. Internationalen Symposiums zur deutschen Kultur und Geschichte im europäischen Nordosten vom 20.-22. September 2001 in Tallinn

    Index Scriptorium Estoniae

    Lawaty, Andreas

    2011-01-01

    Arvustus : Nordosteuropa als Geschichtsregion. Beiträge des III. Internationalen Symposiums zur deutschen Kultur und Geschichte im europäischen Nordosten vom 20.-22. September 2001 in Tallinn. Lübeck : Verlag Schmidt-Römhild 2006. (Veröffentlihungen der Aue-Stiftung. 17)

  9. (SSR) markers

    African Journals Online (AJOL)

    Yomi

    2012-04-03

    Apr 3, 2012 ... erucic acid and seed glucosinolate. The grouping of accessions .... DNA extraction. The plants of all accessions were cultivated for one month in the field. Leaves from 3 to 5 seedlings for each accession were pooled together for DNA isolation. Genomic DNA was extracted according to the protocol of Doyle ...

  10. (SSR) markers

    African Journals Online (AJOL)

    SAM

    2014-07-30

    Jul 30, 2014 ... al., 2004; Lefebvre et al., 2001; Moon et al., 2003; Paran et al., 1998; Prince et al., 1992). These markers have proven to be very useful in assessing genetic diversity and phylogeny, characterization of germplasm and detection of duplicates, parental verification in crosses, gene tagging in marker assisted ...

  11. (SSR) markers

    African Journals Online (AJOL)

    aghomotsegin

    Unknown. Unknown. V. vinifera L. 2000s. Chile. Stary goru. Ancient variety of Japan. V. vinifera L. 1980s. Japan. Medoc Noir. Unknown. V. vinifera L. 1980s. France. Vidal Blanc. Ugni blanc × seyval blanc. V. vinifera L. 1940s. France. Table 2. Summary of genetic variation statistics for the 19 simple sequence repeat markers ...

  12. Eesti autobiograafilise kirjutuse kujunemisest 18. sajandist Teise maailmasõjani. The Development of Estonian Autobiographical Writing from the 18th Century to the Second World War

    Directory of Open Access Journals (Sweden)

    Rutt Hinrikus

    2012-04-01

    Full Text Available In this article I examine the development of Estonian autobiographical writing from its first manifestations to published memoirs, and the development of life writing and its diversification. The beginnings of life writing can be traced back to Estonian folk song and Estonian incidental poetry. The Moravian Brethren movement in Estonia in the 18th century promoted the spread of canonical autobiography. The Moravian Brethren offered alternative opportunities for self-realisation for Estonians who were serfs, and were therefore popular with the people. The practice of the Moravian Brethren made use of retelling and writing about the life of the congregation members, which sometimes became suitable biographies in print, especially stories of awakening. Several manuscript biographies have survived from the Brethren times, such as the biographies of Mäletu Jaan and Mihkel Sarapuu. In addition to the history of the Moravian Brethren movement, these biographies give information about the educational situation and living conditions of the people of the time. The Estonian life writing tradition emerged within the reigning Baltic German cultural space thanks to the Estophiles among the Baltic Germans (J. H. Rosenplänter and the first Estonian men of letters; from the early 19th century we have the diary by Rosenplänter, an estophile pastor from Pärnu, and the diary by the Estonian poet, the then-student Kristjan Jaak Peterson, both in the Estonian language. Johann Voldemar Jannsen, the founder of Estonian-language journalism, kept a diary in the German language for a longer period of time; it was usual that the first Estonian intellectuals (Lilli Suburg, and others in the late 19th century wrote in German. Admittedly, the first Estonian-language life history was written by a forward-looking 19th century peasant named Märt Mitt (1833-1912, who was conscious of himself as a historical subject and gave his memoirs, begun in the 1880s, a memorable title

  13. Aaviku Housing, Tallinn

    Index Scriptorium Estoniae

    2005-01-01

    Arhitektibüroo Emil Urbel OÜ projekteeritud Tallinna Aaviku elamurajoon märgiti ära arhitektuurikonkursil "Parim Euroopas - värv". Eestist kuulus nominente esitanud žüriisse arhitekt Markus Kaasik

  14. Wow! Tallinn! / Jaak Rand

    Index Scriptorium Estoniae

    Rand, Jaak

    2006-01-01

    Jüri Sillarti mängufilm "Kuldrannake". Kunagise retrobändi kokkutulekut kujutava filmi stsenarist on Hans Luik oma näidendi "Tõe hetk" põhjal, operaator on Mait Mäekivi, produtsendid Kristian Taska ja Hans H. Luik, tootjafirmaks Taska Productions. Lisatud Jüri Sillarti filmograafia lk. 83

  15. Three vibrio-resistance related EST-SSR markers revealed by selective genotyping in the clam Meretrix meretrix.

    Science.gov (United States)

    Nie, Qing; Yue, Xin; Chai, Xueliang; Wang, Hongxia; Liu, Baozhong

    2013-08-01

    The clam Meretrix meretrix is an important commercial bivalve distributed in the coastal areas of South and Southeast Asia. In this study, marker-trait association analyses were performed based on the stock materials of M. meretrix with different vibrio-resistance profile obtained by selective breeding. Forty-eight EST-SSR markers were screened and 27 polymorphic SSRs of them were genotyped in the clam stocks with different resistance to Vibrio parahaemolyticus (11-R and 11-S) and to Vibrio harveyi (09-R and 09-C). Allele frequency distributions of the SSRs among different stocks were compared using Pearson's Chi-square test, and three functional EST-SSR markers (MM959, MM4765 and MM8364) were found to be associated with vibrio-resistance trait. The 140-bp allele of MM959 and 128-bp allele of MM4765 had significantly higher frequencies in resistant groups (11-R and 09-R) than in susceptive/control groups (11-S and 09-C) (P markers were consistent with the three subgroups distinctions. The putative functions of contig959, contig4765 and contig8364 also suggested that the three SSR-involved genes might play important roles in immunity of M. meretrix. All these results supported that EST-SSR markers MM959, MM4765 and MM8364 were associated with vibrio-resistance and would be useful for marker-assisted selection (MAS) in M. meretrix genetic breeding. Copyright © 2013 Elsevier Ltd. All rights reserved.

  16. Assessment of Genetic Variation within Commercial Iranian Pomegranate (Punica granatum L. Cultivars, Using ISSR and SSR Markers

    Directory of Open Access Journals (Sweden)

    Meysam Madadi

    2017-07-01

    Full Text Available Pomegranate is one of the most important horticultural crops in Iran, and has been cultivated for thousands of years in this country. At this period due to selection of superior cultivars from nature or mutation emerged in these cultivars, and their vegetative propagation, substantial genetic variation has occurred within and among the cultivars. Thus, each cultivar may consist of different clones. According to this issue, diversity within four commercial cultivars of pomegranate was analyzed. Two molecular marker systems including ISSR and SSR were used to evaluate variability between 36 samples of four commercial cultivars. ISSR markers produced 114 amplification products, out of which 97 were polymorphic (83.23%. Mean resolving power was 2.96 for ISSR markers. 19 SSR molecular markers were used, 15 of which amplified polymorphic products, while the remaining ones monomorphic., The number of polymorphic alleles per locus ranged from two to four (average 3.6. The observed and expected heterozygosities ranged from 0.04 to 0.92 and 0.14 to 0.62, respectively. In addition, mean polymorphic information content was 0.45 for SSR loci. Our results showed that commercial Iranian pomegranate have different clones. Therefore, ISSR and SSR markers can be a useful tools for detecting clones of each cultivar.

  17. Fluorescence- and capillary electrophoresis (CE)-based SSR DNA fingerprinting and a molecular identity database for the Louisiana sugarcane industry

    Science.gov (United States)

    A database of Louisiana sugarcane molecular identity has been constructed and is being updated annually using FAM or HEX or NED fluorescence- and capillary electrophoresis (CE)-based microsatellite (SSR) fingerprinting information. The fingerprints are PCR-amplified from leaf DNA samples of current ...

  18. 78 FR 11939 - Social Security Ruling, SSR 13-2p.; Titles II and XVI: Evaluating Cases Involving Drug Addiction...

    Science.gov (United States)

    2013-02-20

    ...'' to ``addiction'' in the forthcoming DSM-V. For this SSR, there is no substantive difference between... as defined in the latest edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM) published by the American Psychiatric Association.\\3\\ See Question 4. In general, the DSM defines Substance...

  19. The use of sequence-based SSR mining for the development of a vast collection of microsatellites in Aquilegia Formosa

    Science.gov (United States)

    Brandon Schlautman; Vera Pfeiffer; Juan Zalapa; Johanne Brunet

    2014-01-01

    Numerous microsatellite markers were developed for Aquilegia formosafrom sequences deposited within the Expressed Sequence Tag (EST), Genomic Survey Sequence (GSS), and Nucleotide databases in NCBI. Microsatellites (SSRs) were identified and primers were designed for 9 SSR containing sequences in the Nucleotide database, 3803 sequences in the EST...

  20. Language Policy, Tacit Knowledge, and Institutional Learning: The Case of the Swiss Public Service Broadcaster SRG SSR

    Science.gov (United States)

    Perrin, Daniel

    2011-01-01

    "Promoting public understanding" is what the programming mandate asks the Swiss public broadcasting company SRG SSR to do. From a sociolinguistic perspective, this means linking speech communities with other speech communities, both between and within the German-, French-, Italian-, and Romansh-speaking parts of Switzerland. In the…

  1. Identification of QTLs Associated with Callogenesis and Embryogenesis in Oil Palm Using Genetic Linkage Maps Improved with SSR Markers.

    NARCIS (Netherlands)

    Ting, N.C.; Jansen, J.; Nagappan, J.; Ishak, Z.; Chin, C.W.; Tan, S.G.; Cheah, S.C.; Singh, R.

    2013-01-01

    Clonal reproduction of oil palm by means of tissue culture is a very inefficient process. Tissue culturability is known to be genotype dependent with some genotypes being more amenable to tissue culture than others. In this study, genetic linkage maps enriched with simple sequence repeat (SSR)

  2. Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.

    Science.gov (United States)

    Gong, L; Stift, G; Kofler, R; Pachner, M; Lelley, T

    2008-06-01

    Until recently, only a few microsatellites have been available for Cucurbita, thus their development is highly desirable. The Austrian oil-pumpkin variety Gleisdorfer Olkürbis (C. pepo subsp. pepo) and the C. moschata cultivar Soler (Puerto Rico) were used for SSR development. SSR-enriched partial genomic libraries were established and 2,400 clones were sequenced. Of these 1,058 (44%) contained an SSR at least four repeats long. Primers were designed for 532 SSRs; 500 primer pairs produced fragments of expected size. Of these, 405 (81%) amplified polymorphic fragments in a set of 12 genotypes: three C. moschata, one C. ecuadorensis, and eight C. pepo representing all eight cultivar groups. On an average, C. pepo and C. moschata produced 3.3 alleles per primer pair, showing high inter-species transferability. There were 187 SSR markers detecting polymorphism between the USA oil-pumpkin variety "Lady Godiva" (O5) and the Italian crookneck variety "Bianco Friulano" (CN), which are the parents of our previous F(2) mapping population. It has been used to construct the first published C. pepo map, containing mainly RAPD and AFLP markers. Now the updated map comprises 178 SSRs, 244 AFLPs, 230 RAPDs, five SCARs, and two morphological traits (h and B). It contains 20 linkage groups with a map density of 2.9 cM. The observed genome coverage (Co) is 86.8%.

  3. SSR analysis demonstrates that olive production in the southern Marmara region in Turkey uses a single genotype.

    Science.gov (United States)

    Ipek, A; Barut, E; Gulen, H; Oz, A T; Tangu, N A; Ipek, M

    2009-10-20

    The southern Marmara region in Turkey was surveyed to determine the olive cultivars that are used for olive production. Genetic diversity analysis using simple sequence repeat (SSR) markers indicated that the cultivar Gemlik is the major olive cultivar grown in this region, while other olive cultivars are grown only for use as pollinators of Gemlik or for growers' own exotic consumption. Although the quality of Gemlik is widely accepted in Turkey, its tendency toward alternate bearing is a major drawback. Twenty-four genotypes were selected within the cultivar Gemlik because of their tolerance to alternate bearing. These selected genotypes have the same SSR alleles as Gemlik, making them good candidates for developing a Gemlik olive with reduced alternate bearing. About 8% of samples did not share the same SSR alleles with Gemlik, though these genotypes were identified as Gemlik by the growers. Some other standard cultivars that are grown in other regions of Turkey were mistakenly called Gemlik in this region, probably due to the popularity of this cultivar in the southern Marmara region. In conclusion, as indicated by SSR analysis, Gemlik has become the standard cultivar in this region; future research should be focused on techniques to improve the production and quality of table olives and olive oil from this cultivar.

  4. 78 FR 8217 - Social Security Ruling, SSR 13-1p; Titles II and XVI: Agency Processes for Addressing Allegations...

    Science.gov (United States)

    2013-02-05

    ... SOCIAL SECURITY ADMINISTRATION [Docket No. SSA-2012-0071] Social Security Ruling, SSR 13-1p... Administration. ACTION: Notice of Social Security Ruling; Correction. SUMMARY: The Social Security Administration published a document in the Federal Register of January 29, 2013, in FR Doc. 2013-01833, on page 6168, in...

  5. 78 FR 9987 - Social Security Ruling, SSR 13-1p; Titles II and XVI: Agency Processes for Addressing Allegations...

    Science.gov (United States)

    2013-02-12

    ... SOCIAL SECURITY ADMINISTRATION [Docket No. SSA-2012-0071] Social Security Ruling, SSR 13-1p... Administration. ACTION: Notice of Social Security Ruling; Correction. SUMMARY: The Social Security Administration published a document in the Federal Register of January 29, 2013, in FR Doc. 2013-01833, on page 6168, in...

  6. 78 FR 22361 - Social Security Ruling, SSR 13-1p; Titles II and XVI: Agency Processes for Addressing Allegations...

    Science.gov (United States)

    2013-04-15

    ... SOCIAL SECURITY ADMINISTRATION [Docket No. SSA-2012-0071] Social Security Ruling, SSR 13-1p... Administration. ACTION: Notice of Social Security Ruling; Correction. SUMMARY: The Social Security Administration published a document in the Federal Register of January 29, 2013, in FR Doc. 2013-01833, on page 6171, in...

  7. Assessment of genetic diversity in Brassica juncea Brassicaceae genotypes using phenotypic differences and SSR markers

    Directory of Open Access Journals (Sweden)

    Vinu V.

    2013-12-01

    Full Text Available Las especies de mostaza del género Brassica representan uno de los cultivos de semillas oleaginosas más importantes en India, sin embargo, su diversidad genética es poco conocida. Para la utilización de genotipos en programas de cultivos resulta esencial un mayor conocimiento sobre este tema. Debido a ello, se evaluó la diversidad genética entre 44 genotipos de mostaza de la India Brassica juncea incluyendo variedades y líneas puras de diferentes zonas agro-climáticas de la India y algunos genotipos exóticos Australia, Polonia y China. Para ello, se utilizaron marcadores SSR específicos del genoma A y B y datos fenotípicos del rendimiento de 12 cosechas y sus características. De los 143 primers evaluados, 134 reportaron polimorfismo y un total de 355 alelos fueron amplificados. Se generaron dendrogramas a partir de los coeficientes de similitud de Jaccard y de disimilitud Manhattan, basados en un algoritmo de vinculación promedio UPGMA. Se utilizaron datos de marcadores genéticos y datos fenotípicos. Los genotipos se agruparon en cuatro grupos basados en las distancias genéticas. Ambos patrones de agrupamiento, tanto los basados en los coeficientes de similitud de Jaccard como los basados en los de disimilitud Manhattan, separaron independientemente los genotipos por su genealogía y origen, de una manera efectiva. El PCoA reveló que la agrupación de genotipos basada en datos de marcadores SSR, es más convincente que los datos fenotípicos, sin embargo, se observó que la correlación entre las matrices de distancia fenotípica y genética resultó muy baja r=0.11. Por lo tanto, para estudios de diversidad basados en marcadores moleculares es necesario realizar más pruebas. Los marcadores SSR constituyen herramientas más eficientes para discriminar entre genotipos de B. juncea, que las características cuantitativas.

  8. Analysis of simple sequence repeats in rice bean (Vigna umbellata using an SSR-enriched library

    Directory of Open Access Journals (Sweden)

    Lixia Wang

    2016-02-01

    Full Text Available Rice bean (Vigna umbellata Thunb., a warm-season annual legume, is grown in Asia mainly for dried grain or fodder and plays an important role in human and animal nutrition because the grains are rich in protein and some essential fatty acids and minerals. With the aim of expediting the genetic improvement of rice bean, we initiated a project to develop genomic resources and tools for molecular breeding in this little-known but important crop. Here we report the construction of an SSR-enriched genomic library from DNA extracted from pooled young leaf tissues of 22 rice bean genotypes and developing SSR markers. In 433,562 reads generated by a Roche 454 GS-FLX sequencer, we identified 261,458 SSRs, of which 48.8% were of compound form. Dinucleotide repeats were predominant with an absolute proportion of 81.6%, followed by trinucleotides (17.8%. Other types together accounted for 0.6%. The motif AC/GT accounted for 77.7% of the total, followed by AAG/CTT (14.3%, and all others accounted for 12.0%. Among the flanking sequences, 2928 matched putative genes or gene models in the protein database of Arabidopsis thaliana, corresponding with 608 non-redundant Gene Ontology terms. Of these sequences, 11.2% were involved in cellular components, 24.2% were involved molecular functions, and 64.6% were associated with biological processes. Based on homolog analysis, 1595 flanking sequences were similar to mung bean and 500 to common bean genomic sequences. Comparative mapping was conducted using 350 sequences homologous to both mung bean and common bean sequences. Finally, a set of primer pairs were designed, and a validation test showed that 58 of 220 new primers can be used in rice bean and 53 can be transferred to mung bean. However, only 11 were polymorphic when tested on 32 rice bean varieties. We propose that this study lays the groundwork for developing novel SSR markers and will enhance the mapping of qualitative and quantitative traits and marker

  9. Transcriptome sequencing of field pea and faba bean for discovery and validation of SSR genetic markers

    Directory of Open Access Journals (Sweden)

    Kaur Sukhjiwan

    2012-03-01

    Full Text Available Abstract Background Field pea (Pisum sativum L. and faba bean (Vicia faba L. are cool-season grain legume species that provide rich sources of food for humans and fodder for livestock. To date, both species have been relative 'genomic orphans' due to limited availability of genetic and genomic information. A significant enrichment of genomic resources is consequently required in order to understand the genetic architecture of important agronomic traits, and to support germplasm enhancement, genetic diversity, population structure and demographic studies. Results cDNA samples obtained from various tissue types of specific field pea and faba bean genotypes were sequenced using 454 Roche GS FLX Titanium technology. A total of 720,324 and 304,680 reads for field pea and faba bean, respectively, were de novo assembled to generate sets of 70,682 and 60,440 unigenes. Consensus sequences were compared against the genome of the model legume species Medicago truncatula Gaertn., as well as that of the more distantly related, but better-characterised genome of Arabidopsis thaliana L.. In comparison to M. truncatula coding sequences, 11,737 and 10,179 unique hits were obtained from field pea and faba bean. Totals of 22,057 field pea and 18,052 faba bean unigenes were subsequently annotated from GenBank. Comparison to the genome of soybean (Glycine max L. resulted in 19,451 unique hits for field pea and 16,497 unique hits for faba bean, corresponding to c. 35% and 30% of the known gene space, respectively. Simple sequence repeat (SSR-containing expressed sequence tags (ESTs were identified from consensus sequences, and totals of 2,397 and 802 primer pairs were designed for field pea and faba bean. Subsets of 96 EST-SSR markers were screened for validation across modest panels of field pea and faba bean cultivars, as well as related non-domesticated species. For field pea, 86 primer pairs successfully obtained amplification products from one or more template

  10. Field Performance of Five Soybean Mutants Under Drought Stress Conditions and Molecular Analysis Using SSR Markers

    Directory of Open Access Journals (Sweden)

    Y Yuliasti

    2017-08-01

    Full Text Available The objectives of this research wereto evaluate (1 the performance of soybean mutant lines under drought stress conditions, and(2 the genetic diversity and relationship among the mutant lines using SSR markers.The field evaluation was conducted during the dry season of 2011 and 2012 at the experimental Farm of Mataram University, West Nusa Tenggara, Indonesia. The field experiment was set up in a randomized block design. Ten mutant lines and two control varieties were evaluated in four replications. Genetic distance among evaluated lines were determined based on allelic diversity analysis using 40 simple sequence repeat (SSR loci. Under drought stress conditions, two mutant lines, Kdl3 and Kdl8,showed a better performance compared to the other ones. The high yielding mutant lines were Kdl3and Kdl8, which yielded 1.75 t ha-1and 1.69 t ha-1, respectively, compared to the parent and national control, Panderman 1.43 t ha-1 and Muria 1.32 t ha-1. These mutant linesrequired 30.75 to 32days to flower and 79.75 to 83.75 day to harvest with relatively short plant height 28.25 and 23.35 cmrespectively. Those mutant characters were better than those of the other three mutants, the original parents, and the control soybean species. Since the evaluated soybean mutant lines yielded more under drought stress conditions than the standard varieties, they can be used and registered as drought-tolerant soybean mutants. Moreover, the evaluated soybean accessions showed a wide genetic distance. The accessions were clustered into two groups according to their genetic background, namelygroup I (the Panderman with three mutant lines and group II (the Muria with two mutant lines. Twenty-three out of 40 evaluated SSR loci, including AW31, BE806, CMAC7L, S080, S126, S57, S171, S224, S285, S294, S393, S294, S383, S511, S511, S520, S540, S547, S551, S571, S577, and S578, provided polymorphic alleles between the parents and their mutants and could be used to differentiate

  11. Effect of the bradykinin 1 receptor antagonist SSR240612 after oral administration in Mycobacterium tuberculosis-infected mice.

    Science.gov (United States)

    Rodrigues-Junior, Valnês S; Pail, Priscilla B; Villela, Anne D; Falcão, Virgínia C A; Dadda, Adílio S; Abbadi, Bruno L; Pesquero, João B; Santos, Diógenes S; Basso, Luiz A; Campos, Maria M

    2018-03-01

    The role, if any, played by the kinin system in tuberculosis infection models, either in vivo or in vitro, was investigated. The effects of Mycobacterium tuberculosis infection on C57BL/6 wild type, B 1 R-/-, B 2 R-/- and double B 1 R/B 2 R knockout mice were evaluated. Immunohistochemistry analysis was carried out to assess B 1 R and B 2 R expression in spleens and lungs of M. tuberculosis-infected mice. In addition, in vitro experiments with M. tuberculosis-infected macrophages were performed. The in vivo effects of HOE-140 and SSR240612 on the mice model of infection were also evaluated. Infected B 2 R-/- mice exhibited increased splenomegaly, whereas decreased spleen weight in infected double B 1 R/B 2 R knockout mice was observed. The bacterial load, determined as colony-forming units, did not differ in the spleens and lungs of the studied mouse strains. Importantly, immunohistochemical analysis revealed that B 1 R was upregulated in both spleens and lungs of infected mice. M. tuberculosis-infected macrophages incubated with SSR240612, alone or in combination with des-Arg 9 -BK, for four days, displayed a marked inhibitory effect on CFU counts. However, the pre-incubation of the selective B 1 R (des-Arg 9 -BK and SSR240612) and B 2 R (BK and HOE-140) agonists and antagonists, respectively, did not significantly affect the bacterial loads. A statistically significant reduction in the CFU of M. tuberculosis in lungs and spleens of animals treated with SSR240612, but not with HOE-140, was observed. Further efforts should be pursued to clarify whether or not SSR240612 might be considered an option for the treatment of tuberculosis. Copyright © 2018. Published by Elsevier Ltd.

  12. Computation of Estonian CORS data using Bernese 5.2 and Gipsy 6.4 softwares

    Science.gov (United States)

    Kollo, Karin; Kall, Tarmo; Liibusk, Aive

    2017-04-01

    GNSS permanent station network in Estonia (ESTREF) was established already in 2007. In 2014-15 extensive reconstruction of ESTREF was carried out, including the establishment of 18 new stations, change of the hardware in CORS stations as well as establishing GNSS-RTK service for the whole Estonia. For GNSS-RTK service one needs precise coordinates in well-defined reference frame, i.e., ETRS89. For long time stability of stations and time-series analysis the re-processing of Estonian CORS data is ongoing. We re-process data from 2007 until 2015 with program Bernese GNSS 5.2 (Dach, 2015). For the set of ESTREF stations established in 2007, we perform as well computations with GIPSY 6.4 software (Ries et al., 2015). In the computations daily GPS-only solution was used. For precise orbits, final products from CODE (CODE analysis centre at the Astronomical Institute of the University of Bern) and JPL (Jet Propulsion Laboratory) for Bernese and GIPSY solutions were used, respectively. The cut-off angle was set to 10 degrees in order to avoid near-field multipath influence. In GIPSY, precise point positioning method with fixing ambiguities was used. Bernese calculations were performed based on double difference processing. Antenna phase centers were modelled based on igs08.atx and epnc_08.atx files. Vienna mapping function was used for mapping tropospheric delays. For the GIPSY solution, the higher order ionospheric term was modelled based on IRI-2012b model. For the Bernese solution higher order ionospheric term was neglected. FES2004 ocean tide loading model was used for the both computation strategies. As a result, two solutions using different scientific GNSS computation programs were obtained. The results from Bernese and GIPSY solutions were compared, using station repeatability values, RMS and coordinate differences. KEYWORDS: GNSS reference station network, Bernese GNSS 5.2, Gipsy 6.4, Estonia. References: Dach, R., S. Lutz, P. Walser, P. Fridez (Eds); 2015

  13. Conservation and synteny of SSR loci and QTLs for vegetative propagation in four Eucalyptus species.

    Science.gov (United States)

    Marques, M.; Brondani, V.; Grattapaglia, D.; Sederoff, R.

    2002-08-01

    Conservation of microsatellite loci, heterozygous in Eucalyptus grandis, Eucalyptus urophylla, Eucalyptus tereticornis and Eucalyptus globulus, allowed us to propose homeologies among genetic linkage groups in these species, supported by at least three SSR loci in two different linkage groups. Marker-trait associations for sprouting and adventitious rooting ability were also compared in the four species. Putative quantitative trait loci (QTLs) influencing vegetative propagation traits were located on homeologous linkage groups. Our findings indicate high transferability of microsatellite markers between Eucalyptus species of the Symphyomyrtus subgenus and establish foundations for the use of synteny in the genetic analysis of this genus. Microsatellite markers should help integrate eucalypt genetic linkage maps from various sources. The availability of comparative linkage maps provides a basis of more-efficient use of genetic information for molecular breeding and evolutionary studies in Eucalyptus.

  14. Epidemiology of allergic diseases of the respiratory passages in the Kazakh SSR

    Energy Technology Data Exchange (ETDEWEB)

    Moshkevich, V.S.

    1985-01-01

    Over a period of 20 years, the authors have been studying the distribution, aetiology and causes of increasing incidence of allergic respiratory diseases in various climatogeographic zones of the Kazakh SSR. Large groups of people living in towns and in the country were examined by various methods. The number of patients seeking advice in health service establishments because of allergic rhinitis and bronchial asthma was found to increase every year. A number of factors influencing the incidence of disease were pointed out, such as the character of diet, duration of the person's stay, vaccination against brucellosis, pollution of the atmosphere, local flora, climate, and other factors. Morbidity also depended on the methods of studying the epidemiology of respiratory allergoses. The obtained results will help health service authorities in taking specific measures to reduce morbidity from the mentioned pathological condition.

  15. SuperSafe Reactor (SSR): a supercritical water-cooled small reactor

    Energy Technology Data Exchange (ETDEWEB)

    Yetisir, M.; Gaudet, M., E-mail: yetisirm@aecl.ca [Atomic Energy of Canada Limited, Chalk River, Ontario (Canada); Duffey, R. [DSM Associates Inc., Idaho (United States)

    2013-07-01

    A new small modular reactor (SMR) is presented for a 300 MW(e) nuclear generating station, which is referred to as the SuperSafe Reactor© (SSR). The SSR is a smaller version of the Canadian supercritical water-cooled reactor (SCWR), which is designed to operate at supercritical conditions (pressure of 25 MPa and fluid temperatures of up to 625{sup o}C) at the turbine inlet with a high cycle efficiency of greater than 45%. The supercritical turbine technology and associated components used in the balance of plant are similar to and derived from existing supercritical fossil-fired plants. To avoid a large pressure vessel subject to supercritical water pressures and for enhanced safety, the reactor core consists of multiple fuel channels, which are submerged in a subcooled heavy-water moderator pool inside a low-pressure calandria vessel. Each fuel channel consists of a pressure tube, a ceramic insulator and a fuel bundle assembly. Energy from nuclear fission at normal operating conditions is used to heat the light water coolant to the supercritical state so that very high thermal efficiencies can be achieved. To provide inherent safety, the moderator provides additional cooling to fuel channels under postulated accident scenarios. This design feature also enables the use of a natural circulation flashing-flow driven passive moderator cooling. Another inherent safety feature of the proposed design and a major safety goal is to achieve a passive 'no core melt' configuration for the channels and fuel. (author)

  16. SSR allelic variation of rice variety Hangxiangnuo bred by space mutation

    International Nuclear Information System (INIS)

    Yang Tifeng; Liu Chuanguang; Pan Dajian; Fan Zhilan; Li Chen; Chen Jianyou; Liu Bin; Jiang Yijun; Gao Yun; Zhou Hanqin

    2011-01-01

    Hangxiangnuo, an indica fragrant glutinous rice mutant, was induced by space environment. Comparing with its wild type Nanfengnuo, the yield and blast resistance of Hangxiangnuo are improved significantly and the grain shape became slender and with fragrance. To understand the mechanisms of space mutation and identify the changes at molecular level associated with phenotypic variations, SSR allelic variation analysis were performed on Hangxiangnuo and Nanfengnuo in this study. The results showed that 45 loci were polymorphic among the 156 SSR loci tested throughout the genome, the frequency of variation was 28.85%. Among the polymorphic loci, 42 loci only showed variations in the molecular weight of the amplified bands, only on locus increased the number of amplification bands in Hangxiangnuo and two loci were differed by heterozygous loci (with two amplification bands at one locus) detected in Nanfengnuo and homozygous loci in Hangxiangnuo. It suggests that the change of some loci in mutants was due to the normal segregation and recombination of heterozygous loci of the wild type. The variation frequencies among different chromosomes were quite different, with the highest one at 50.00% detected on chromosomes 7, 8 and 12, and the lowest at 6.25% on chromosome 6. The polymorphic loci were clustered on chromosomes throughout the genome indicating that large DNA segments mutation is one of the major variation patterns induced by space environment. Some of reported QTLs involved in grain shape, yield, fragrance and blast resistance were found to be located exactly in the mutated regions. Therefore, further study is needed to confirm that these QTLs are responsible for the trait variations. (authors)

  17. Assessment of Genetic Diversity and Population Genetic Structure of Corylus mandshurica in China Using SSR Markers.

    Directory of Open Access Journals (Sweden)

    Jian-Wei Zong

    Full Text Available Corylus mandshurica, also known as pilose hazelnut, is an economically and ecologically important species in China. In this study, ten polymorphic simple sequence repeat (SSR markers were applied to evaluate the genetic diversity and population structure of 348 C. mandshurica individuals among 12 populations in China. The SSR markers expressed a relatively high level of genetic diversity (Na = 15.3, Ne = 5.6604, I = 1.8853, Ho = 0.6668, and He = 0.7777. According to the coefficient of genetic differentiation (Fst = 0.1215, genetic variation within the populations (87.85% were remarkably higher than among populations (12.15%. The average gene flow (Nm = 1.8080 significantly impacts the genetic structure of C. mandshurica populations. The relatively high gene flow (Nm = 1.8080 among wild C. mandshurica may be caused by wind-pollinated flowers, highly nutritious seeds and self-incompatible mating system. The UPGMA (unweighted pair group method of arithmetic averages dendrogram was divided into two main clusters. Moreover, the results of STRUCTURE analysis suggested that C. mandshurica populations fell into two main clusters. Comparison of the UPGMA dendrogram and the Bayesian STRUCTURE analysis showed general agreement between the population subdivisions and the genetic relationships among populations of C. mandshurica. Group I accessions were located in Northeast China, while Group II accessions were in North China. It is worth noting that a number of genetically similar populations were located in the same geographic region. The results further showed that there was obvious genetic differentiation among populations from Northeast China to North China. Results from the Mantel test showed a weak but still significant positive correlation between Nei's genetic distance and geographic distance (km among populations (r = 0.419, P = 0.005, suggesting that genetic differentiation in the 12 C. mandshurica populations might be related to geographic

  18. Genetic variability of a Brazilian Capsicum frutescens germplasm collection using morphological characteristics and SSR markers.

    Science.gov (United States)

    Carvalho, S I C; Bianchetti, L B; Ragassi, C F; Ribeiro, C S C; Reifschneider, F J B; Buso, G S C; Faleiro, F G

    2017-07-06

    Characterization studies provide essential information for the conservation and use of germplasm in plant breeding programs. In this study, 103 Capsicum frutescens L. accessions from the Active Germplasm Bank of Embrapa Hortaliças, representative of all five Brazilian geographic regions, were characterized based on morphological characteristics and microsatellite (or simple sequence repeat - SSR) molecular markers. Morphological characterization was carried out using 57 descriptors, and molecular characterization was based on 239 alleles from 24 microsatellite loci. From the estimates of genetic distances among accessions, based on molecular characterization, a cluster analysis was carried out, and a dendrogram was established. Correlations between morphological and molecular variables were also estimated. Twelve morphological descriptors were monomorphic for the set of C. frutescens accessions, and those with the highest degree of polymorphism were stem length (14.0 to 62.0 cm), stem diameter (1.0 to 4.2 cm), days to flowering (90 to 129), days to fruiting (100 to 140), fruit weight (0.1 to 1.4 g), fruit length (0.6 to 4.6 cm), and fruit wall thickness (0.25 to 1.5 mm). The polymorphism information content for the SSR loci varied from 0.36 (EPMS 417) to 0.75 (CA49), with an overall mean of 0.57. The correlation value between morphological and molecular characterization data was 0.6604, which was statistically significant. Fourteen accessions were described as belonging to the morphological type tabasco, 85 were described as malagueta, and four were malaguetinha, a morphological type confirmed in this study. The typical morphological pattern of malagueta was described. Six similarity groups were established for C. frutescens based on the dendrogram and are discussed individually. The genetic variability analyzed in the study highlights the importance of characterizing genetic resources available for the development of new C. frutescens cultivars with the potential

  19. Assessment of Genetic Diversity in Opuntia spp. Portuguese Populations Using SSR Molecular Markers

    Directory of Open Access Journals (Sweden)

    Carlos M. G. Reis

    2018-04-01

    Full Text Available The Opuntia spp., most likely few individuals, were introduced in the Iberian Peninsula in the beginning of the 16th century, after the discovery of America, spreading afterwards throughout the Mediterranean basin. We analysed, for the first time, the genetic diversity in a set of 19 Portuguese Opuntia spp. populations from the species O. ficus-indica, O. elata, O. dillenii and O. robusta using nuclear microsatellite (nuSSR markers. The Italian cultivars ‘Bianca’, ‘Gialla’ and ‘Rossa’ were included in the study for comparison purposes. The nuSSR amplifications produced from five to 16 alleles, with an average of 9.2 alleles per primer pair, and average polymorphism information content of 0.71. The estimated Dice coefficient among populations varied from 0.26 to 1.0, indicating high interspecific genetic diversity but low genetic diversity at the intraspecific level. The hierarchical clustering analysis revealed four major groups that clearly separated the four Opuntia species. Among the O. ficus-indica populations, two sub-clusters were found, one including the white pulp fruits (with cv. Bianca and the other with the orange pulp ones and including the cv. Gialla, the cv. Rossa, and one pale yellow pulp population. No genetic differences were found between the inermis form, O. ficus-indica f. ficus-indica, and the rewilded spiny one, O. ficus-indica f. amyclaea. The dendrogram indicated that the clustering pattern was unrelated to geographical origin. The results revealed a low level of genetic diversity among the Portuguese populations of O. ficus-indica.

  20. Development and Integration of an SSR-Based Molecular Identity Database into Sugarcane Breeding Program

    Directory of Open Access Journals (Sweden)

    Yong-Bao Pan

    2016-04-01

    Full Text Available Sugarcane breeding is very difficult and it takes 12 to 14 years to develop a new cultivar for commercial production. This is because sugarcane varieties are highly polyploid, inter-specific hybrids with 100 to 130 chromosomes that may vary across geographical areas. Other obstacles/constraints include the small size of flowers that may not synchronize but may self-pollinate, difficulty in distinguishing hybrids from self progenies, extreme (G × E interactive effect, and potential variety mis-identification during vegetative propagation and varietal exchange. To help cane breeders circumvent these constraints, a simple sequence repeats (SSR-based molecular identity database has been developed at the United States Department of Agriculture-Agricultural Research Service, Sugarcane Research Unit in Houma, LA. Since 2005, approximately 2000 molecular identities have been constructed for clones of sugarcane and related Saccharum species that cover geographical areas including Argentina, Australia, Bangladesh, China, Colombia, India, Mexico, Pakistan, South Africa, Thailand, USA (Louisiana, Florida, Texas, and Hawaii, and Venezuela. The molecular identity database is updated annually and has been utilized to: (1 provide molecular descriptors to newly registered cultivars; (2 identify in a timely fashion any mislabeled or unidentifiable clones from cross parents and field evaluation plots; (3 develop de novo clones of energy cane with S. spontaneum cytoplasm; (4 provide clone-specific fingerprint information for assessing cross quality and paternity of polycross; (5 determine genetic relatedness of parental clones; (6 select F1 hybrids from (elite × wild or (wild × elite crosses; and (7 investigate the inheritance of SSR markers in sugarcane. The integration of the molecular identity database into the sugarcane breeding program may improve the overall efficacy of cultivar development and commercialization.

  1. GENETIC DIVERSITY OF UPLAND RICE LANDRACES FROM JAVA ISLAND AS REVEALED BY SSR MARKERS

    Directory of Open Access Journals (Sweden)

    Sutoro Sutoro

    2015-09-01

    Full Text Available Java Island is one of origins of a large number of indigenous upland rice accessions, which may serve as valuable plant genetic resources for future crop improvement in Indonesia. However, these landraces especially non-glutinous and glutinous rice are rapidly being lost because of land-use, agricultural practices and other factors. A better understanding of genetic diversity of local upland rice is important for crop improvement program, crop management and conservation strategy. This study aimed to evaluate the genetic diversity of upland rice landraces originating from Java Island. A total of 82 upland rice accessions comprising of 55 non-glutinous rice and 27 glutinous type were genotyped using the 16 simple sequence repeat (SSR markers. The result showed that a total of 74 alleles were found with major allele frequency found on RM431 (0.96. Most of the SSR markers (56.3% showed high discriminating power as represented by polymorphic informa-tion content (PIC value higher than 0.5. A moderate genetic diversity index was detected in all landraces, which was 0.55. Genetic diversity index of non-glutinous and glutinous rice were 0.54 and 0.53, respectively. Their genetic distance was about 0.057. The phylogenetic tree generated two main clusters that demonstrated discrimination among landraces according to the individual genetic properties rather than their geographical origins and grain types (non-glutinous and glutinous type. The levels of genetic diversity were varied across rice types and geographical origins. According to the regions, the closest genetic distance was found between upland rice landraces from Central Java and West Java (0.040. The information derived from this study is important, in combination with phenotypic data, to identify desired useful traits came from different origins of the gene pool to be used for breeding purposes.

  2. Report on the development of putative functional SSR and SNP markers in passion fruits.

    Science.gov (United States)

    da Costa, Zirlane Portugal; Munhoz, Carla de Freitas; Vieira, Maria Lucia Carneiro

    2017-09-06

    Passionflowers Passiflora edulis and Passiflora alata are diploid, outcrossing and understudied fruit bearing species. In Brazil, passion fruit cultivation began relatively recently and has earned the country an outstanding position as the world's top producer of passion fruit. The fruit's main economic value lies in the production of juice, an essential exotic ingredient in juice blends. Currently, crop improvement strategies, including those for underexploited tropical species, tend to incorporate molecular genetic approaches. In this study, we examined a set of P. edulis transcripts expressed in response to infection by Xanthomonas axonopodis, (the passion fruit's main bacterial pathogen that attacks the vines), aiming at the development of putative functional markers, i.e. SSRs (simple sequence repeats) and SNPs (single nucleotide polymorphisms). A total of 210 microsatellites were found in 998 sequences, and trinucleotide repeats were found to be the most frequent (31.4%). Of the sequences selected for designing primers, 80.9% could be used to develop SSR markers, and 60.6% SNP markers for P. alata. SNPs were all biallelic and found within 15 gene fragments of P. alata. Overall, gene fragments generated 10,003 bp. SNP frequency was estimated as one SNP every 294 bp. Polymorphism rates revealed by SSR and SNP loci were 29.4 and 53.6%, respectively. Passiflora edulis transcripts were useful for the development of putative functional markers for P. alata, suggesting a certain level of sequence conservation between these cultivated species. The markers developed herein could be used for genetic mapping purposes and also in diversity studies.

  3. Evaluation of rice germplasm under salt stress at the seedling stage through SSR markers

    Directory of Open Access Journals (Sweden)

    M. Al-Amin

    2013-06-01

    Full Text Available Twenty eight rice germplasms were used for identification of salt tolerant rice genotypes at the seedling stage at the experimental farm and Biotechnology laboratory of the Bangladesh Institute of Nuclear Agriculture (BINA, Mymensingh during February 2009 to October 2009. Phenotyping for salinity screening of the rice genotypes was done using salinized (EC level 12 dS m-1 nutrient solution in hydroponic system. Genotypes were evaluated for salinity tolerance on 1-9 scale based on seedling growth parameters following modified Standard Evaluation Scoring (SES of IRRI. Phenotypically, on the basis of SES and % total dry matter (TDM reduction of the genotypes viz. PBSAL-614, PBSAL-613, PBSAL-730, Horkuch, S-478/3 Pokkali and PBSAL (STL-15 were found to be salt tolerant; on the other hand Iratom-24, S-653/32, S-612/32, S-604/32, S-633/32, Charnock (DA6, BINA Dhan-6 and S-608/32 were identified as salt susceptible. For genotyping, ten SSR markers were used for polymorphism, where 3 primers (RM127, RM443 and RM140 were selected for evaluation of salt tolerance. In respect of Primer RM127, 7 lines were found salt tolerant and 11 lines were moderately tolerant and 10 lines were susceptible. Nine tolerant, 9 moderately tolerant and 10 susceptible lines were found when the primer RM140 was used and primer RM443 identified 8 lines as tolerant, 9 lines as moderately tolerant and 11 lines as susceptible. Thus, the salt tolerant lines can be used in further evaluation for salinity tolerance and the SSR markers used in this study are proving valuable for identifying salt tolerant genes in marker assisted breeding.

  4. Antimicrobial Resistance Profiles of Campylobacter spp. Isolated from Broiler Chicken Meat of Estonian, Latvian and Lithuanian Origin at Estonian Retail Level and from Patients with Severe Enteric Infections in Estonia.

    Science.gov (United States)

    Mäesaar, M; Kramarenko, T; Meremäe, K; Sõgel, J; Lillenberg, M; Häkkinen, L; Ivanova, M; Kovalenko, K; Hörman, A; Hänninen, M-L; Roasto, M

    2016-03-01

    The resistance patterns of Campylobacter spp. isolated from retail broiler chicken meat originating either from Estonia, Lithuania or Latvia collected in Estonia were determined. Additionally, in collaboration with the laboratories of several Estonian hospitals, antimicrobial susceptibility patterns were determined for Campylobacter isolates from patients with severe Campylobacter enteric infections. The isolates were identified at the species level by the PCR method. Respectively, 88.8% of the isolates were C. jejuni, and 11.2% were C. coli. In total, 126 Campylobacter isolates of broiler chicken meat and human origin were tested for minimal inhibitory concentrations (MICs) with the broth microdilution VetMIC(TH) method (National Veterinary Institute; Uppsala, Sweden) for a total of six antimicrobials. Resistance to one or more antimicrobials was detected in 62 (63.3%) of Campylobacter broiler chicken meat isolates and in 20 (71.4%) of human-origin isolates. Large proportions of the broiler chicken meat isolates were resistant to ciprofloxacin (60.2%). Multidrug resistance (i.e. to three or more unrelated antimicrobials) was detected in five (5.1%) C. jejuni isolates. Among the human isolates, 20 (71.4%) were resistant to fluoroquinolones, and two (7.1%) C. jejuni isolates exhibited multidrug resistance. The chicken meat isolates of Estonian origin were the most susceptible. However, a high proportion of fluoroquinolone-resistant C. jejuni isolates were found in Latvian and Lithuanian products. The results of this study indicate that the problems caused by the inappropriate use of antimicrobials extend beyond the country in which a food originates; therefore, both domestic and international interventions and agreements are required to implement common policies on antimicrobial usage and to minimize the emergence of Campylobacter drug resistance. © 2015 Blackwell Verlag GmbH.

  5. Chemical composition of anthropogenic particles on needles collected close to the Estonian oil-shale power plants

    International Nuclear Information System (INIS)

    Meinander, O.

    1995-01-01

    Within the countries surrounding the Baltic Sea, north-eastern Estonia is among the most polluted areas. Emissions from the oil-shale power plants produce air pollution problems both locally and on a larger scale. In the atmosphere, pollutants mix and convert. Consequently, the particles deposited due to the use of oil-shale can have various chemical compositions. From the point of view of air chemistry, ecological effects and air pollution modelling, knowledge of the chemical composition of the deposited particles can be of great value. The aim of this work was to study the chemical composition of single anthropogenic particles occurring on needle surfaces in north-eastern Estonia and Southern Finland close to the Estonian oil-shale power plants. For the purpose, scanning electron microscopical microanalysis was used

  6. The Problems of Estonian R&D and Innovation Strategy and the Demand-Side Innovation Policies

    Directory of Open Access Journals (Sweden)

    Tõnu Roolaht

    2012-12-01

    Full Text Available The second larger Estonian R&D and Innovation Strategy ‘Knowledge-based Estonia 2007-2013’ is aimed at continuing the advancement of research and development efforts towards an innovative knowledge-based society and economic system in Estonia. Fostering of knowledge-based high-tech industries is seen as paramount for retaining country’s competitive advantage. However, the mid-term evaluations indicate that several goals of the strategy might not be achievable by 2013. In fact, the policy measures have been much more successful in developing scientific research, as indicated by increased international publication, number of patents, and number of researchers and engineers. The advances in development of high-tech products and services through innovations are noticeable but less prominent. The purpose of this study is to suggest the role for demand-side innovation policies in helping to advance commercial development and innovation

  7. Individual and work-related risk factors for musculoskeletal pain: a cross-sectional study among Estonian computer users

    Science.gov (United States)

    2014-01-01

    Background Occupational use of computers has increased rapidly over recent decades, and has been linked with various musculoskeletal disorders, which are now the most commonly diagnosed occupational diseases in Estonia. The aim of this study was to assess the prevalence of musculoskeletal pain (MSP) by anatomical region during the past 12 months and to investigate its association with personal characteristics and work-related risk factors among Estonian office workers using computers. Methods In a cross-sectional survey, the questionnaires were sent to the 415 computer users. Data were collected by self-administered questionnaire from 202 computer users at two universities in Estonia. The questionnaire asked about MSP at different anatomical sites, and potential individual and work related risk factors. Associations with risk factors were assessed by logistic regression. Results Most respondents (77%) reported MSP in at least one anatomical region during the past 12 months. Most prevalent was pain in the neck (51%), followed by low back pain (42%), wrist/hand pain (35%) and shoulder pain (30%). Older age, right-handedness, not currently smoking, emotional exhaustion, belief that musculoskeletal problems are commonly caused by work, and low job security were the statistically significant risk factors for MSP in different anatomical sites. Conclusions A high prevalence of MSP in the neck, low back, wrist/arm and shoulder was observed among Estonian computer users. Psychosocial risk factors were broadly consistent with those reported from elsewhere. While computer users should be aware of ergonomic techniques that can make their work easier and more comfortable, presenting computer use as a serious health hazard may modify health beliefs in a way that is unhelpful. PMID:24884911

  8. Tõlkepärl eesti ilukirjanduse algusaegadest – esimene eestikeelne robinsonaad / A Translation Gem from the Beginnings of Estonian Literature - the First Robinsonade

    Directory of Open Access Journals (Sweden)

    Ave Mattheus

    2015-06-01

    Full Text Available Teesid: Artiklis uuritakse Eesti Kirjandusmuuseumis asuvat mahukat, ligi 800 lk tõlkekäsikirja „Norem Robinson“, mida võib pidada esimeseks eesti kirjanduse täiemahuliseks robinsonaadiks. Selle valmistas Pärnu koolmeister Heinrich Gottlieb Lorenzsonn saksa pedagoogi ja koolikirjaniku Joachim Heinrich Campe menukast noorsooromaanist „Robinson der Jüngere“ (1779–1780. Tõlge valmis 1822.–1823. aastal, kuid jõudis trükki alles 1842. aastal tugevasti kärbitud ja mugandatud kujul. Toetudes deskriptiivse tõlkeuurimuse analüüsikategooriatele, vaadeldakse artiklis, millised tegurid tõlkeprotsessi suunasid ja milline oli kultuuriruum, kuhu tõlge omal ajal paigutus. SU M M A R Y This article discusses a voluminous manuscript translation of almost 800 pages entitled Norem Robinson (Engl. Robinson the Younger, from the collections of the Estonian Literary Museum. This manuscript can be considered as the first complete Robinsonade in Estonian literature. Its author is a schoolteacher from Pärnu, Heinrich Gottlieb Lorenzsonn (1803–1847, who translated it from the youth novel Robinson der Jüngere(1779–1780, Engl. Robinson the Younger, a bestseller by the educator, writer and a major representative of German Enlightenment, Heinrich Joachim Campe. Lorenzsonn’s translation was completed in 1822–1823, but not printed until 1842 in a strongly adapted version titled Norema Robinsoni ello ja juhtumised ühhe tühja sare peäl (Engl. The Life and Adventures of Robinson the Younger on a deserted island. The print version of the Robinsonade lacks a pedagogical frame story, where the father tells children about the adventures of Robinson and takes the opportunity to discuss and imitate with children all the actions taken by Robinson the Younger. Due to this and other extirpated parts, the possible target audience was enlarged – besides children and youth, the text was now addressed to adults as well. In accordance with the

  9. Transcriptome sequencing of mung bean (Vigna radiate L. genes and the identification of EST-SSR markers.

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    Honglin Chen

    Full Text Available Mung bean (Vigna radiate (L. Wilczek is an important traditional food legume crop, with high economic and nutritional value. It is widely grown in China and other Asian countries. Despite its importance, genomic information is currently unavailable for this crop plant species or some of its close relatives in the Vigna genus. In this study, more than 103 million high quality cDNA sequence reads were obtained from mung bean using Illumina paired-end sequencing technology. The processed reads were assembled into 48,693 unigenes with an average length of 874 bp. Of these unigenes, 25,820 (53.0% and 23,235 (47.7% showed significant similarity to proteins in the NCBI non-redundant protein and nucleotide sequence databases, respectively. Furthermore, 19,242 (39.5% could be classified into gene ontology categories, 18,316 (37.6% into Swiss-Prot categories and 10,918 (22.4% into KOG database categories (E-value < 1.0E-5. A total of 6,585 (8.3% were mapped onto 244 pathways using the Kyoto Encyclopedia of Genes and Genome (KEGG pathway database. Among the unigenes, 10,053 sequences contained a unique simple sequence repeat (SSR, and 2,303 sequences contained more than one SSR together in the same expressed sequence tag (EST. A total of 13,134 EST-SSRs were identified as potential molecular markers, with mono-nucleotide A/T repeats being the most abundant motif class and G/C repeats being rare. In this SSR analysis, we found five main repeat motifs: AG/CT (30.8%, GAA/TTC (12.6%, AAAT/ATTT (6.8%, AAAAT/ATTTT (6.2% and AAAAAT/ATTTTT (1.9%. A total of 200 SSR loci were randomly selected for validation by PCR amplification as EST-SSR markers. Of these, 66 marker primer pairs produced reproducible amplicons that were polymorphic among 31 mung bean accessions selected from diverse geographical locations. The large number of SSR-containing sequences found in this study will be valuable for the construction of a high-resolution genetic linkage maps, association

  10. The effect of hormone therapy on women's quality of life in the first year of the Estonian Postmenopausal Hormone Therapy trial

    OpenAIRE

    Veerus, Piret; Hovi, Sirpa-Liisa; Sévon, Tiina; Hunter, Myra; Hemminki, Elina

    2012-01-01

    Background For postmenopausal women, the main reason to start hormone therapy (HT) is to reduce menopausal symptoms and to improve quality of life (QOL). The aim of this study was to analyse the impact of HT on different aspects of symptom experience and QOL during a randomised trial. A total of 1823 postmenopausal women were recruited into the Estonian Postmenopausal Hormone Therapy (EPHT) trial in 1999–2001. Women were randomised to blind HT, open-label HT, placebo or non-treatment ...

  11. THE CHOICES BETWEEN FAIR VALUE AND HISTORICAL COST ACCOUNTING FOR NON-CURRENT REAL ASSETS AT LATVIAN, LITHUANIAN AND ESTONIAN LISTED COMPANIES DURING THE REPORTING YEAR OF 2011

    OpenAIRE

    Justine Jaunzeme

    2013-01-01

    Stock investors require financial statements of listed companies for their investment decision-making. J. Gassen and K. Schwedler (2008) have empirically proved that EU investors who use financial statements prefer reported assets’ fair values over historical costs. The purpose of this study is to test whether the Latvian, Lithuanian and Estonian listed companies set accounting policies for their non-current real assets in favor of EU investors who prefer fair values over historical costs. Th...

  12. Genetic diversity analysis of Amomum tsao-ko in Jinping County of Yunnan Province using SSR markers

    Science.gov (United States)

    Ma, Mengli; Wang, Tiantao; Lei, En; Meng, Hengling; Xie, Linyan; Zhu, Kunlong; Duan, Shaoze; Li, Wenqiang; Lu, Bingyue

    2017-08-01

    Genetic diversity analysis is very important for germplasm resources conservation and utilization. The objective of this study was to assess the genetic diversity among 44 individuals of Amomum tsao-ko from Jinping County of Yunnan Province using 5 selected SSR (simple sequence repeat) markers. A total of 23 polymorphic loci were detected among these germplasms, with an average of 4.6 polymorphic loci per SSR primer combination. The percentage of polymorphic loci was 100%, whereas the mean effective number of alleles (Ne), observed heterozygosity(Ho), expected heterozygosity (He), Shannon's information index (I), and the mean polymorphism information content (PIC) were 3. 410, 0. 491, 0. 679, 1.266 and 0. 672, respectively, indicating that the Amomum tsao-ko germplasms from Jinping County had high genetic diversity.

  13. Development and characterization of 59 polymorphic cDNA-SSR markers for the edible oil crop Sesamum indicum (Pedaliaceae).

    Science.gov (United States)

    Wang, Linhai; Zhang, Yanxin; Qi, Xiaoqiong; Gao, Yuan; Zhang, Xiurong

    2012-10-01

    Polymorphic simple sequence repeat markers from transcript sequences (cDNA-simple sequence repeat [SSR]) were developed for the edible oil crop Sesamum indicum to facilitate the genetic study of this species. • We found 7702 SSR loci in the 60960 unigenes, and 1550 primer pairs were designed and synthesized. In total, 59 primer pairs showed polymorphism within 36 individuals; the number of alleles per locus ranged from two to four, and the expected and observed heterozygosity ranged from 0.05 to 0.74 and 0 to 0.30, respectively. • These polymorphic markers will greatly facilitate studies of the genetic structure of S. indicum populations as well as the identification and conservation of the species.

  14. Association of SSR markers with contents of fatty acids in olive oil and genetic diversity analysis of an olive core collection.

    Science.gov (United States)

    Ipek, M; Ipek, A; Seker, M; Gul, M K

    2015-03-27

    The purpose of this research was to characterize an olive core collection using some agronomic characters and simple sequence repeat (SSR) markers and to determine SSR markers associated with the content of fatty acids in olive oil. SSR marker analysis demonstrated the presence of a high amount of genetic variation between the olive cultivars analyzed. A UPGMA dendrogram demonstrated that olive cultivars did not cluster on the basis of their geographic origin. Fatty acid components of olive oil in these cultivars were determined. The results also showed that there was a great amount of variation between the olive cultivars in terms of fatty acid composition. For example, oleic acid content ranged from 57.76 to 76.9% with standard deviation of 5.10%. Significant correlations between fatty acids of olive oil were observed. For instance, a very high negative correlation (-0.812) between oleic and linoleic acids was detected. A structured association analysis between the content of fatty acids in olive oil and SSR markers was performed. STRUCTURE analysis assigned olive cultivars to two gene pools (K = 2). Assignment of olive cultivars to these gene pools was not based on geographical origin. Association between fatty acid traits and SSR markers was evaluated using the general linear model of TASSEL. Significant associations were determined between five SSR markers and stearic, oleic, linoleic, and linolenic acids of olive oil. Very high associations (P olive.

  15. Non-market value of Estonian seminatural grasslands: a contingent valuation study. Eesti poolloodusliku rohumaa turuväline väärtus: tingliku hindamise uuring

    Directory of Open Access Journals (Sweden)

    Helli Lepasaar

    2015-12-01

    Full Text Available Seminatural grasslands i.e. the floodplain meadows, seashore meadows, wooded meadows, dry meadows, wooded pastures are the very traditional part of Estonian landscapes, which play an important role in the appearance of the landscape in general and also serve as an important habitat for many plant and animal species. In order to preserve the seminatural grasslands continuous annual mowing and/or pasturing is needed. This activity is not economically profitable and needs subsidizing. The authors of the work raise a hypothesis that the Estonian seminatural grasslands could be viewed as a valuable non-market environmental good for which a significant public demand exists. In order to find out the non-market value of the seminatural grasslands a contingent valuation study was carried out among the Estonian working-age population (size of the sample 1061 individuals. The average individual willingness to pay was 11.3 euros. During the study, the authors constructed the total demand function and discovered that the total annual demand for seminatural grasslands was 17.9 million euros.

  16. Comparative analysis of genetic diversity among Chinese watermelon germplasmsusing SSR and SRAP markers, and implications for future genetic improvement

    OpenAIRE

    WANG, PANGQIAO; LI, QIONG; Hu, Jianbin; SU, YAN

    2015-01-01

    The genetic diversity of watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] in China, the world's largest producer of watermelon fruits, has not been examined. Two molecular markers, sequence-related amplified polymorphism (SRAP) and simple sequence repeat (SSR), were used to investigate the genetic variation and genetic relationship among 54 Chinese watermelon accessions, as well as 7 accessions from Africa, the United States, and Japan. SRAP assay generated 312 bands, ...

  17. Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.

    Science.gov (United States)

    Gong, L.; Stift, G.; Kofler, R.; Pachner, M.

    2008-01-01

    Until recently, only a few microsatellites have been available for Cucurbita, thus their development is highly desirable. The Austrian oil-pumpkin variety Gleisdorfer Ölkürbis (C. pepo subsp. pepo) and the C. moschata cultivar Soler (Puerto Rico) were used for SSR development. SSR-enriched partial genomic libraries were established and 2,400 clones were sequenced. Of these 1,058 (44%) contained an SSR at least four repeats long. Primers were designed for 532 SSRs; 500 primer pairs produced fragments of expected size. Of these, 405 (81%) amplified polymorphic fragments in a set of 12 genotypes: three C. moschata, one C. ecuadorensis, and eight C. pepo representing all eight cultivar groups. On an average, C. pepo and C. moschata produced 3.3 alleles per primer pair, showing high inter-species transferability. There were 187 SSR markers detecting polymorphism between the USA oil-pumpkin variety “Lady Godiva” (O5) and the Italian crookneck variety “Bianco Friulano” (CN), which are the parents of our previous F2 mapping population. It has been used to construct the first published C. pepo map, containing mainly RAPD and AFLP markers. Now the updated map comprises 178 SSRs, 244 AFLPs, 230 RAPDs, five SCARs, and two morphological traits (h and B). It contains 20 linkage groups with a map density of 2.9 cM. The observed genome coverage (Co) is 86.8%. Electronic supplementary material The online version of this article (doi:10.1007/s00122-008-0750-2) contains supplementary material, which is available to authorized users. PMID:18379753

  18. Variability of red rot-resistant somaclones of sugarcane genotype S97US297 assessed by RAPD and SSR.

    Science.gov (United States)

    Shahid, M T H; Khan, F A; Saeed, A; Fareed, I

    2011-08-26

    Sugarcane breeding under climatic conditions of Pakistan is very difficult due to unavailability of viable fuzz (seed). Somaclonal variation can provide an alternative for improvement of existing genotypes. Six hundred and twenty-seven somaclones were developed from sugarcane genotype S97US297, and protocols for callogenesis and organogenesis were developed using Murashige and Skoog medium. Two types of explants, leaf and pith, and two auxins, 2,4-dichlorophenoxy acetic acid and indole-3-acetic acid, were tested to optimize callogenesis for root establishment. Leaves as explants with 3.0 mg/L 2,4-dichlorophenoxy acetic acid gave the best results, both for callus induction and proliferation. Half-strength Murashige and Skoog medium with 1.5 mg/L indole-3-butyric acid proved to be the best for rooting. Red rot-resistant somaclones of the R(2) generation along with the parent were assessed for genetic variability at the molecular level using RAPD and SSR markers. Polymorphism based on RAPD and SSR was 32 and 67%, respectively. Polymorphic information content ranged from 0.06-0.45 for RAPD and 0.06-0.47 for SSR. We conclude that somaclonal variation of sugarcane varieties is sufficient to allow selection.

  19. Transferability of simple sequence repeat (SSR) markers developed in guava (Psidium guajava L.) to four Myrtaceae species.

    Science.gov (United States)

    Rai, Manoj K; Phulwaria, Mahendra; Shekhawat, N S

    2013-08-01

    Present study demonstrated the cross-genera transferability of 23 simple sequence repeat (SSR) primer pairs developed for guava (Psidium guajava L.) to four new targets, two species of eucalypts (Eucalyptus citriodora, Eucalyptus camaldulensis), bottlebrush (Callistemon lanceolatus) and clove (Syzygium aromaticum), belonging to the family Myrtaceae and subfamily Myrtoideae. Off the 23 SSR loci assayed, 18 (78.2%) gave cross-amplification in E. citriodora, 14 (60.8%) in E. camaldulensis and 17-17 (73.9%) in C. lanceolatus and S. aromaticum. Eight primer pairs were found to be transferable to all four species. The number of alleles detected at each locus ranged from one to nine, with an average of 4.8, 2.6, 4.5 and 4.6 alleles in E. citriodora, E. camaldulensis, C. lanceolatus and S. aromaticum, respectively. The high levels of cross-genera transferability of guava SSRs may be applicable for the analysis of intra- and inter specific genetic diversity of target species, especially in E. citriodora, C. lanceolatus and S. aromaticum, for which till date no information about EST-derived as well as genomic SSR is available.

  20. Genetic Diversity of Namibian Pennisetum glaucum (L. R. BR. (Pearl Millet Landraces Analyzed by SSR and Morphological Markers

    Directory of Open Access Journals (Sweden)

    Billy McBenedict

    2016-01-01

    Full Text Available Current Pennisetum glaucum (L. R. BR. cultivars in Namibia have overall poor performance posing a threat to the nation’s food security because this crop is staple for over 70% of the Namibian population. The crop suffers from undesirable production traits such as susceptibility to diseases, low yield, and prolonged reproductive cycle. This study aimed to understand the genetic diversity of the crop in Namibia by simple sequence repeats (SSRs and morphology analysis. A total of 1441 genotypes were collected from the National Gene Bank representing all the Namibian landraces. A sample of 96 genotypes was further analyzed by SSR using Shannon-Wiener diversity index and revealed a value of 0.45 indicating low genetic diversity. Ordination using Principal Coordinate Analysis (PCoA on SSR data confirmed clusters generated by UPGMA for the 96 P. glaucum accessions. UPGMA phenograms of 29 morphological characterized genotypes were generated for SSR and morphology data and the two trees revealed 78% resemblance. Lodging susceptibility, tillering attitude, spike density, fodder yield potential, early vigour, and spike shape were the phenotypic characters upon which some clusters were based in both datasets. It is recommended that efforts should be made to widen the current gene pool in Namibia.

  1. Rahvusliku ajaloo tõlgendusi eesti draamas ja teatris 1970.–1980. aastatel. Interpretations of National History in Estonian Drama and Theatre in the 1970s–1980s

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    Piret Kruuspere

    2012-04-01

    Full Text Available According to theatre scholar Freddie Rokem, theatre portraying or performing historical events is seeking to overcome both the separation and the exclusion from the past, as well as ’striving to create a community where the events from this past will matter again’. This article covers the topic of interpretations of national history in Estonian theatre and in original dramaturgy during the Soviet era, with the focus on aspects like national self-reflection and the relationship to the common past. The main focus is on the 1970s, with examples from Rein Saluri’s, Mati Unt’s and Jaan Kaplinski’s drama productions. During the period in question, re-tellings of national history on Estonian theatre stages were clothed in metaphors, allusions and secret codes – Aesopian language. Within the Soviet cultural context, I analyse if and to what extent theatre of the time displayed resistance, political theatre or social allegory. Theatre was also connected with the principle of playing or playfulness, which on one hand indicates national resistance, national endurance, and a certain survival strategy, but on the other hand indicates the Estonian as being an involuntary homo ludens – the Playing Man, who through various enforced roles is trying to adjust to the whirlwinds of history. Saluri’s first play, the intellectual drama Külalised (The Guests, opens with an allusion to a drama classic the world over, William Shakespeare’s Hamlet, adding a powerful national-symbolic background to the play. The role-play which permeates and structures the play (The Host–The Guest however, displays allegorical references to changes in the status and self-image of Estonians. In Unt’s play Peaproov (Dress Rehearsal, the principle of playing/acting sheds ironic light on the makers of an historical film and their readiness to create superficially flashy interpretations at any cost: this take acts as an estranging and generalising reflection in a context

  2. A second generation framework for the analysis of microsatellites in expressed sequence tags and the development of EST-SSR markers for a conifer, Cryptomeria japonica

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    Ueno Saneyoshi

    2012-04-01

    Full Text Available Abstract Background Microsatellites or simple sequence repeats (SSRs in expressed sequence tags (ESTs are useful resources for genome analysis because of their abundance, functionality and polymorphism. The advent of commercial second generation sequencing machines has lead to new strategies for developing EST-SSR markers, necessitating the development of bioinformatic framework that can keep pace with the increasing quality and quantity of sequence data produced. We describe an open scheme for analyzing ESTs and developing EST-SSR markers from reads collected by Sanger sequencing and pyrosequencing of sugi (Cryptomeria japonica. Results We collected 141,097 sequence reads by Sanger sequencing and 1,333,444 by pyrosequencing. After trimming contaminant and low quality sequences, 118,319 Sanger and 1,201,150 pyrosequencing reads were passed to the MIRA assembler, generating 81,284 contigs that were analysed for SSRs. 4,059 SSRs were found in 3,694 (4.54% contigs, giving an SSR frequency lower than that in seven other plant species with gene indices (5.4–21.9%. The average GC content of the SSR-containing contigs was 41.55%, compared to 40.23% for all contigs. Tri-SSRs were the most common SSRs; the most common motif was AT, which was found in 655 (46.3% di-SSRs, followed by the AAG motif, found in 342 (25.9% tri-SSRs. Most (72.8% tri-SSRs were in coding regions, but 55.6% of the di-SSRs were in non-coding regions; the AT motif was most abundant in 3′ untranslated regions. Gene ontology (GO annotations showed that six GO terms were significantly overrepresented within SSR-containing contigs. Forty–four EST-SSR markers were developed from 192 primer pairs using two pipelines: read2Marker and the newly-developed CMiB, which combines several open tools. Markers resulting from both pipelines showed no differences in PCR success rate and polymorphisms, but PCR success and polymorphism were significantly affected by the expected PCR product size

  3. Characterization of the global transcriptome for cotton (Gossypium hirsutum L.) anther and development of SSR marker.

    Science.gov (United States)

    Zhang, Xianwen; Ye, Zhenwei; Wang, Tiankang; Xiong, Hairong; Yuan, Xiaoling; Zhang, Zhigang; Yuan, Youlu; Liu, Zhi

    2014-11-10

    Cotton is an important fiber plant, and it's attractive to elucidate the molecular mechanism of anther development due to the close relationship between the anther fertility and boll-setting, and also fiber yield. In the present paper, 47.2 million paired-end reads with average length of 82.87 bp from the anthers of TM-1 (Gossypium hirsutum L.), a genetic standard line, were generated through transcriptome sequencing, and 210,965 unigenes of more than 100 bp were obtained. BLAST, KEGG, COG, and GO analyses showed that the genes were enriched in the processes of transcription, translation, and post-translation as well as hormone signal transduction, the transcription factor families, and cell wall-related genes mainly participating in cell expansion and carbohydrate metabolism. Further analysis identified 11,153 potential SSRs. A suit of 5122 primer pair sequences were designed, and 82 of 300 randomly selected primer pairs produced reproducible amplicons that were polymorphic among 22 cotton accessions from G. hirsutum, Gossypium barbadense and Gossypium arboreum. The UPGMA clustering analysis further confirmed high quality and effectiveness of these novel SSR markers. The present study provided insights into the transcriptome profile of the cotton and established a public information platform for functional genomics and molecular breeding. Copyright © 2014 Elsevier B.V. All rights reserved.

  4. Design And Development Of An Automatic Single Phase Protective Device Using Ssr

    Directory of Open Access Journals (Sweden)

    Michael E.

    2017-10-01

    Full Text Available Since the discovery of energy safety has been a paramount subject matter. This we can see in todays electrical systems where protective devices such as fuse and circuit breakers are used to prevent fire hazards resulting from overload overvoltage and short circuits. However with all the revolution in technology these options may be considered less smart since the fuse made with wire strands calculated for specific current capacity faults permanently when the specified current rating is exceeded. While the circuit breaker which is made up of mechanical switch fails as a result of carbon forming and the wearing away of the contacts because of arcing. As a means of improvement this paper presents the design and development of an automatic single phase protective device using solid state relay SSR. This study is to ensure automatic cut off from power supply in cases of overvoltage above 240 V AC or when overload and short circuit current above 8amps is detected without permanent damage of a fuse placed along current path. Also the design will ensure that there is an automatic close circuit whenever the trigger switch is momentary switch is closed. The system is achieved via the use of PIC micro-controller current sensor and other discrete components. The system is tested and works well inhibiting the frequent faulting of fuses. It also helps to prevent hazard as a result of overvoltage overload and short circuit and ensures a close circuit when the trigger switch is closed.

  5. Foundation characteristics of edible Musa triploids revealed from allelic distribution of SSR markers.

    Science.gov (United States)

    Hippolyte, I; Jenny, C; Gardes, L; Bakry, F; Rivallan, R; Pomies, V; Cubry, P; Tomekpe, K; Risterucci, A M; Roux, N; Rouard, M; Arnaud, E; Kolesnikova-Allen, M; Perrier, X

    2012-04-01

    The production of triploid banana and plantain (Musa spp.) cultivars with improved characteristics (e.g. greater disease resistance or higher yield), while still preserving the main features of current popular cultivars (e.g. taste and cooking quality), remains a major challenge for Musa breeders. In this regard, breeders require a sound knowledge of the lineage of the current sterile triploid cultivars, to select diploid parents that are able to transmit desirable traits, together with a breeding strategy ensuring final triploidization and sterility. Highly polymorphic single sequence repeats (SSRs) are valuable markers for investigating phylogenetic relationships. Here, the allelic distribution of each of 22 SSR loci across 561 Musa accessions is analysed. We determine the closest diploid progenitors of the triploid 'Cavendish' and 'Gros Michel' subgroups, valuable information for breeding programmes. Nevertheless, in establishing the likely monoclonal origin of the main edible triploid banana subgroups (i.e. 'Cavendish', 'Plantain' and 'Mutika-Lujugira'), we postulated that the huge phenotypic diversity observed within these subgroups did not result from gamete recombination, but rather from epigenetic regulations. This emphasizes the need to investigate the regulatory mechanisms of genome expression on a unique model in the plant kingdom. We also propose experimental standards to compare additional and independent genotyping data for reference.

  6. Genetic diversity of loquat germplasm (Eriobotrya japonica (Thunb) Lindl) assessed by SSR markers.

    Science.gov (United States)

    Soriano, José Miguel; Romero, Carlos; Vilanova, Santiago; Llácer, Gerardo; Badenes, María Luisa

    2005-02-01

    Genetic relationships among 40 loquat (Eriobotrya japonica (Thunb) Lindl) accessions that originated from different countries and that are part of the germplasm collection of the Instituto Valenciano de Investigaciones Agrarias (IVIA) (Valencia, Spain) were evaluated using microsatellites. Thirty primer pairs flanking microsatellites previously identified in Malus x domestica (Borkh.) were assayed. Thirteen of them amplified polymorphic products and unambiguously distinguished 34 genotypes from the 40 accessions analyzed. Six accessions showing identical marker patterns were Spanish local varieties thought to have been derived from 'Algerie' by a mutational process very common in loquat species. A total of 39 alleles were detected in the population studied, with a mean value of 2.4 alleles per locus. The expected and observed heterozygosities were 0.46 and 51% on average, respectively, leading to a negative value of the Wright's fixation index (-0.20). The values of these parameters indicate a smaller degree of genetic diversity in the set of loquat accessions analyzed than in other members of the Rosaceae family. Unweighted pair-group method (UPGMA) cluster analysis, based on Nei's genetic distance, generally grouped genotypes according to their geographic origins and pedigrees. The high number of alleles and the high expected heterozygosity detected with SSR markers developed in Malus x domestica (Borkh.) make them a suitable tool for loquat cultivar identification, confirming microsatellite marker transportability among genera in the Rosaceae family.

  7. Exploration and Exploitation of Novel SSR Markers for Candidate Transcription Factor Genes in Lilium Species

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    Manosh Kumar Biswas

    2018-02-01

    Full Text Available Lilies (Lilium sp. are commercially important horticultural crops widely cultivated for their flowers and bulbs. Here, we conducted large-scale data mining of the lily transcriptome to develop transcription factor (TF-associated microsatellite markers (TFSSRs. Among 216,768 unigenes extracted from our sequence data, 6966 unigenes harbored simple sequence repeats (SSRs. Seventy-one SSRs were associated with TF genes, and these were used to design primers and validate their potential as markers. These 71 SSRs were accomplished with 31 transcription factor families; including bHLH, MYB, C2H2, ERF, C3H, NAC, bZIP, and so on. Fourteen highly polymorphic SSRs were selected based on Polymorphic Information Content (PIC values and used to study genetic diversity and population structure in lily accessions. Higher genetic diversity was observed in Longiflorum compared to Oriental and Asiatic populations. Lily accessions were divided into three sub-populations based in our structure analysis, and an un-rooted neighbor-joining tree effectively separated the accessions according to Asiatic, Oriental, and Longiflorum subgroups. Finally, we showed that 46 of the SSR-associated genes were differentially expressed in response to Botrytis elliptica infection. Thus, our newly developed TFSSR markers represent a powerful tool for large-scale genotyping, high-density and comparative mapping, marker-aided backcrossing, and molecular diversity analysis of Lilium sp.

  8. A Set of 20 New SSR Markers Developed and Evaluated in Mandevilla Lindl.

    Directory of Open Access Journals (Sweden)

    Alev Oder

    2016-09-01

    Full Text Available Mandevilla is an ornamental crop with a bright future worldwide because of its high commercial acceptance and added value. However, as with most ornamental species, there are few molecular tools to support cultivar breeding and innovation. In this work, we report the development and analysis of 20 new Simple Sequence Repeat (SSR markers in Mandevilla. Microsatellites were isolated from two enriched small-insert genomic libraries of Mandevilla × amabilis. The diversity parameters estimated after their amplification in a group of 11 commercial genotypes illustrate the effect of two opposite drifts: the high relatedness of cultivars belonging to the same commercial group and the high divergence of other cultivars, especially M. × amabilis. Based on their different band patterns, six genotypes were uniquely distinguished, and two groups of sport mutations remained undistinguishable. The amplification of the SSRs in three wild species suggested the existence of unexploited diversity available to be introgressed into the commercial pool. This is the first report of available microsatellites in Mandevilla. The development process has provided some clues concerning the genome structure of the species, and the SSRs obtained will help to create new products and to protect existing and upcoming plant innovations.

  9. Genetic Diversity in Jatropha curcas L. Assessed with SSR and SNP Markers

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    Juan M. Montes

    2014-08-01

    Full Text Available Jatropha curcas L. (jatropha is an undomesticated plant that has recently received great attention for its utilization in biofuel production, rehabilitation of wasteland, and rural development. Knowledge of genetic diversity and marker-trait associations is urgently needed for the design of breeding strategies. The main goal of this study was to assess the genetic structure and diversity in jatropha germplasm with co-dominant markers (Simple Sequence Repeats (SSR and Single Nucleotide Polymorphism (SNP in a diverse, worldwide, germplasm panel of 70 accessions. We found a high level of homozygosis in the germplasm that does not correspond to the purely outcrossing mating system assumed to be present in jatropha. We hypothesize that the prevalent mating system of jatropha comprise a high level of self-fertilization and that the outcrossing rate is low. Genetic diversity in accessions from Central America and Mexico was higher than in accession from Africa, Asia, and South America. We identified makers associated with the presence of phorbol esters. We think that the utilization of molecular markers in breeding of jatropha will significantly accelerate the development of improved cultivars.

  10. Keragaman Genetik Kacang Bogor (Vigna subterranea L. Verdc. Berdasarkan Marka SSR (Simple Sequence Repeat

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    Zikril Illahi

    2017-01-01

    Full Text Available ABSTRACTBambara groundnut (Vigna subterranea L. Verdc. is an important underutilized legume crop in Indonesia. The aim of this research is to study genetic diversity of bambara groundnut from Sukabumi and Sumedang, West Java, Indonesia. This study used 107 bambara groundnut accessions, which consisted of 57 accessions from Sukabumi and 50 accessions from Sumedang. We use five simple sequence repeat (SSR to analyze the accessions. Totally nine alleles were detected, with a mean of 1.8 alleles per locus. Allelic and gene diversities were higher in Sumedang (1.8 alleles per locus and 0.119 than in Sukabumi population (1.4 alleles per locus and 0.020, respectively. We constructed a phylogenic tree by Neighbor-Joining analysis based on genetic distances (DA and showed the tree divided bambara groundnut accessions into two broad groups according to the origin of samples (Sukabumi and Sumedang. Results from the phylogenic tree are in line with those from the population structure analysis. Keywords: allele diversity, bambara groundnut, genetic distance, under utilized legume

  11. PROBLEMS OF TRANSPORT COMPLEX STAFFING OF UKRAINIAN SSR IN 1960-1980

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    Anatoliy Gorban

    2016-11-01

    Full Text Available In the article the staffing problems of transport complex in the period of its fundamental technical change and intensive development of 1960–1980-ies are described. The main purpose of this article is to analyze the peculiarities of personnel potential development of transport complex of the Ukrainian SSR in the years of the intensive development of transport sectors, figure out the social component of the measures to improve the efficiency of transport networks. Methodology. In this article we used the method of analysis. The qualitative personnel structure, training of skilled workers in the educational institutions and other forms of improvement of personal skills of transport workers are analyzed. The causes of staff turnover and low labour discipline are studied. Practical implementation. This article shows the possible opportunities for the modern transport complex of Ukraine, how to be successful and competitive in the market. The historical background of this article can be very useful for present managers of the transport complex. Results. The author makes a general conclusion that the main causes of personnel fluctuation in transport brunches are low wages, pour conditions of work and lack of accomodations for the staff. The social and practical measures to improve effectiveness of work with personnel of all brunches of transport system are characterized.

  12. Eesti keele astmevaheldusest ja prosoodiast / Kalevi Wiik ; soomekeelsest käsikirjast tõlkinud Väino Klaus

    Index Scriptorium Estoniae

    Wiik, Kalevi

    1997-01-01

    Arvustus: Hint, Mati. Eesti keele astmevahelduse ja prosoodiasüsteemi tüpoloogilised probleemid : [artiklivalimik] = Typological problems of Estonian grade alternation and prosodical system = Viron kielen astevaihtelun ja prosodian typologiset ongelmat. Tallinn ; Helsinki : Eesti Keele Instituut, 1997

  13. [Mati Hint. Eesti keele astmevahelduse ja prosoodiasüsteemi tüpoloogilised probleemid] / Paul Alvre

    Index Scriptorium Estoniae

    Alvre, Paul

    1997-01-01

    Arvustus: Hint, Mati. Eesti keele astmevahelduse ja prosoodiasüsteemi tüpoloogilised probleemid : [artiklivalimik] = Typological problems of Estonian grade alternation and prosodical system = Viron kielen astevaihtelun ja prosodian typologiset ongelmat. Tallinn ; Helsinki : Eesti Keele Instituut, 1997

  14. Värske kõrvaga kuuldud uuest Eesti muusikast / Elo Masing

    Index Scriptorium Estoniae

    Masing, Elo

    2011-01-01

    Eksperimentaalset Eesti muusikat tutvustava heliplaadi "Estonian Music Now 2011 - Exploratory Music From Estonia" esitluskontserdist 31. oktoobril Londonis Cafe OTOs, kus esinesid duo Taavi Kerikmäe - Mart Soo, Taavo Remmel ning Free Tallinn Trio

  15. Eesti album ehk elu läheb edasi / Igor Kotjuh

    Index Scriptorium Estoniae

    Kotjuh, Igor, 1978-

    2005-01-01

    Arvustus: Semenenko, Svetlan. Estonski albom, ili, Jeshtsho ne vsjo poterjano = Eesti album, ehk, Mitte kõik ei ole veel kaotatud = Estonian album, or, Not everything is lost yet. Tallinn : Ilo, 2004

  16. Tüür, Erkki-Sven: Architectonics VI. Passion. Illusion / Guy S. Rickards

    Index Scriptorium Estoniae

    Rickards, Guy S.

    1996-01-01

    Uuest heliplaadist "Tüür, Erkki-Sven: Architectonics VI. Passion. Illusion. Crystallisatio. Requiem. Estonian Philharmonic Chamber Choir, Tallinn Chamber Orchestra, Tõnu Kaljuste. ECM New Series 449 459-2 (64 minutes: DDD)

  17. Arvo Pärt. Litany / Peter Korfmacher

    Index Scriptorium Estoniae

    Korfmacher, Peter

    1996-01-01

    Uuest heliplaadist "Pärt, Arvo. Litany; Psalom; Trisagion. Hilliard Ensemble, Estonian Philharmonic Chamber Choir, Tallinn Chamber Orchestra, Tõnu Kaljuste"; Lithuanian Chamber Orchestra, Saulius Sondeckis. ECM 1592/449 810-2

  18. Pärt, Arvo. Litany; Psalom; Trisagion / Andreas Obst

    Index Scriptorium Estoniae

    Obst, Andreas

    1996-01-01

    Pärt, Arvo. Litany; Psalom; Trisagion. Hilliard Ensemble, Estonian Philharmonic Chamber Choir, Tallinn Chamber Orchestra, Tõnu Kaljuste; Lithuanian Chamber Orchestra, Saulius Sondeckis. ECM 1592/449 810-2

  19. Pärt, Arvo: Litany. Psalom. Trisagion / Robert Cowan

    Index Scriptorium Estoniae

    Cowan, Robert

    1996-01-01

    Uuest heliplaadist "Pärt, Arvo: Litany. Psalom. Trisagion. Hilliard Ensemble, Estonian Philharmonic Chamber Choir, Tallinn Chamber Orchestra, Tõnu Kaljuste; Lithuanian Chamber Orchestra, Saulius Sondeckis" ECM New Series 449 810-4; 449 810-2 (42 minutes: DDD)

  20. [Joonas Sildre ; Andreas Trossek. Narratiivsus piltides] / Sven Vabar

    Index Scriptorium Estoniae

    Vabar, Sven, 1977-

    2010-01-01

    Arvustus: Trossek, Andreas ; Sildre, Joonas. Narratiivsus piltides. Eesti '00 aastate autorikoomiks. Osa 2 = Narration in pictures. Estonian alternative comics from the '00s. Part 2. Tallinn : Haus Galerii, 2009