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Sample records for tag detection efficiency

  1. Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins.

    Science.gov (United States)

    Takemoto, Y; Sato, M; Furuta, M; Hashimoto, Y

    1997-07-01

    We have generated cDNA expression vectors that efficiently produce tagged proteins. The newly introduced cloning site of this plasmid facilitates subcloning of cDNA in the lambda gt11 phage into the plasmid vector. Because the cDNA is inserted next to the motifs of the tagged DNA sequence, the protein produced by the tag sequence-coupled cDNA is easily detected by Western blot analysis or immunoprecipitation using commercially available antibodies. The double-tagged protein significantly enhances the efficiency of Western blot and immunoprecipitation detection as compared with the single-tagged protein.

  2. Efficiency of Portable Antennas for Detecting Passive Integrated Transponder Tags in Stream-Dwelling Salmonids.

    Directory of Open Access Journals (Sweden)

    Nolan P Banish

    Full Text Available Portable antennas have become an increasingly common technique for tracking fish marked with passive integrated transponder (PIT tags. We used logistic regression to evaluate how species, fish length, and physical habitat characteristics influence portable antenna detection efficiency in stream-dwelling brown trout (Salmo trutta, bull trout (Salvelinus confluentus, and redband trout (Oncorhynchus mykiss newberrii marked with 12-mm PIT tags. We redetected 56% (20/36 of brown trout, 34% (68/202 of bull trout, and 33% (20/61 of redband trout after a recovery period of 21 to 46 hours. Models indicate support for length and species and minor support for percent boulder, large woody debris, and percent cobble as parameters important for describing variation in detection efficiency, although 95% confidence intervals for estimates were large. The odds of detecting brown trout (1.5 ± 2.2 [mean ± SE] are approximately four times as high as bull trout (0.4 ± 1.6 or redband trout (0.3 ± 1.8 and species-specific differences may be related to length. Our reported detection efficiency for brown trout falls within the range of other studies, but is the first reported for bull trout and redband trout. Portable antennas may be a relatively unbiased way of redetecting varying sizes of all three salmonid species.

  3. Efficiency of portable antennas for detecting passive integrated transponder tags in stream-dwelling salmonids

    Science.gov (United States)

    Banish, Nolan P.; Burdick, Summer M.; Moyer, Katherine R.

    2016-01-01

    Portable antennas have become an increasingly common technique for tracking fish marked with passive integrated transponder (PIT) tags. We used logistic regression to evaluate how species, fish length, and physical habitat characteristics influence portable antenna detection efficiency in stream-dwelling brown trout (Salmo trutta), bull trout (Salvelinus confluentus), and redband trout (Oncorhynchus mykiss newberrii) marked with 12-mm PIT tags. We redetected 56% (20/36) of brown trout, 34% (68/202) of bull trout, and 33% (20/61) of redband trout after a recovery period of 21 to 46 hours. Models indicate support for length and species and minor support for percent boulder, large woody debris, and percent cobble as parameters important for describing variation in detection efficiency, although 95% confidence intervals for estimates were large. The odds of detecting brown trout (1.5 ± 2.2 [mean ± SE]) are approximately four times as high as bull trout (0.4 ± 1.6) or redband trout (0.3 ± 1.8) and species-specific differences may be related to length. Our reported detection efficiency for brown trout falls within the range of other studies, but is the first reported for bull trout and redband trout. Portable antennas may be a relatively unbiased way of redetecting varying sizes of all three salmonid species.

  4. Efficient refolding and immobilization of PMMA-tag-fused single-chain Fv antibodies for sensitive immunological detection on a PMMA plate.

    Science.gov (United States)

    Kumada, Yoichi; Ishikawa, Yasuyuki; Fujiwara, Yusuke; Takeda, Rui; Miyamoto, Ryosuke; Niwa, Daisuke; Momose, Shun; Kang, Bongmun; Kishimoto, Michimasa

    2014-09-01

    In this study, we investigated the efficient refolding and site-specific immobilization of single-chain variable fragments (scFvs) genetically fused with a poly(methylmethacrylate)-binding peptide (PMMA-tag). According to the results of an aggregation test of a scFv-PM in the presence of 0.5 M urea, aggregation was hardly detectable at a weak-alkaline pH (8.5) with lower concentrations of NaCl. Consequently, more than 93% recovery of the anti-RNase scFv-PM model was attained, when it was refolded by dialysis against 50 mM TAPS (pH8.5). These results suggested that the apparent isoelectric point (pI) of a target scFv was decreased to a great extent by the genetic fusion of a PMMA-tag containing 5 acidic amino acids, and, thus, the solubility of the scFv-PM in its semi-denatured form was considerably improved. We also designed alternative peptide-tags composed of plural aspartic acid residues (D5, D10 and D15-tags) to decrease the apparent pI value of the fusion protein. As a consequence, scFv-D5, scFv-D10 and scFv-D15 were also efficiently refolded with yields of more than 95%. It is noteworthy that even scFv-PS-D15, which had both a positively charged polystyrene-binding peptide (PS-tag) and a negatively charged D15-tag, was serially connected at the C-terminal region of scFvs, and also refolded with a yield of 96.1%. These results clearly indicate that controlling the apparent pI value of scFvs by the fusion of oligo-peptides composed of acidic amino acids at the C-terminus resulted in a high degree of recovery via dialysis refolding. According to the results of a sandwich ELISA using scFv-PMs, scFv-D15 and scFv-PS-D15 as ligands, high antigen-binding signals were detected from both the PMMA and phi-PS plates immobilized with scFv-PMs. Furthermore, the high antigen-binding activity of scFv-PMs was maintained in an adsorption state when it was immobilized on the surface of not only PMMA, but also hydrophilic PS (phi-PS) and polycarbonate (PC). These results

  5. Novel and efficient tag SNPs selection algorithms.

    Science.gov (United States)

    Chen, Wen-Pei; Hung, Che-Lun; Tsai, Suh-Jen Jane; Lin, Yaw-Ling

    2014-01-01

    SNPs are the most abundant forms of genetic variations amongst species; the association studies between complex diseases and SNPs or haplotypes have received great attention. However, these studies are restricted by the cost of genotyping all SNPs; thus, it is necessary to find smaller subsets, or tag SNPs, representing the rest of the SNPs. In fact, the existing tag SNP selection algorithms are notoriously time-consuming. An efficient algorithm for tag SNP selection was presented, which was applied to analyze the HapMap YRI data. The experimental results show that the proposed algorithm can achieve better performance than the existing tag SNP selection algorithms; in most cases, this proposed algorithm is at least ten times faster than the existing methods. In many cases, when the redundant ratio of the block is high, the proposed algorithm can even be thousands times faster than the previously known methods. Tools and web services for haplotype block analysis integrated by hadoop MapReduce framework are also developed using the proposed algorithm as computation kernels.

  6. Clone tag detection in distributed RFID systems.

    Science.gov (United States)

    Kamaludin, Hazalila; Mahdin, Hairulnizam; Abawajy, Jemal H

    2018-01-01

    Although Radio Frequency Identification (RFID) is poised to displace barcodes, security vulnerabilities pose serious challenges for global adoption of the RFID technology. Specifically, RFID tags are prone to basic cloning and counterfeiting security attacks. A successful cloning of the RFID tags in many commercial applications can lead to many serious problems such as financial losses, brand damage, safety and health of the public. With many industries such as pharmaceutical and businesses deploying RFID technology with a variety of products, it is important to tackle RFID tag cloning problem and improve the resistance of the RFID systems. To this end, we propose an approach for detecting cloned RFID tags in RFID systems with high detection accuracy and minimal overhead thus overcoming practical challenges in existing approaches. The proposed approach is based on consistency of dual hash collisions and modified count-min sketch vector. We evaluated the proposed approach through extensive experiments and compared it with existing baseline approaches in terms of execution time and detection accuracy under varying RFID tag cloning ratio. The results of the experiments show that the proposed approach outperforms the baseline approaches in cloned RFID tag detection accuracy.

  7. Clone tag detection in distributed RFID systems

    Science.gov (United States)

    Kamaludin, Hazalila; Mahdin, Hairulnizam

    2018-01-01

    Although Radio Frequency Identification (RFID) is poised to displace barcodes, security vulnerabilities pose serious challenges for global adoption of the RFID technology. Specifically, RFID tags are prone to basic cloning and counterfeiting security attacks. A successful cloning of the RFID tags in many commercial applications can lead to many serious problems such as financial losses, brand damage, safety and health of the public. With many industries such as pharmaceutical and businesses deploying RFID technology with a variety of products, it is important to tackle RFID tag cloning problem and improve the resistance of the RFID systems. To this end, we propose an approach for detecting cloned RFID tags in RFID systems with high detection accuracy and minimal overhead thus overcoming practical challenges in existing approaches. The proposed approach is based on consistency of dual hash collisions and modified count-min sketch vector. We evaluated the proposed approach through extensive experiments and compared it with existing baseline approaches in terms of execution time and detection accuracy under varying RFID tag cloning ratio. The results of the experiments show that the proposed approach outperforms the baseline approaches in cloned RFID tag detection accuracy. PMID:29565982

  8. Factors Affecting Detection Probability of Acoustic Tags in Coral Reefs

    KAUST Repository

    Bermudez, Edgar F.

    2012-05-01

    Acoustic telemetry is an important tool for studying the movement patterns, behaviour, and site fidelity of marine organisms; however, its application is challenged in coral reef environments where complex topography and intense environmental noise interferes with acoustic signals, and there has been less study. Therefore, it is particularly critical in coral reef telemetry studies to first conduct a long-term range test, a tool that provides informa- tion on the variability and periodicity of the transmitter detection range and the detection probability. A one-month range test of a coded telemetric system was conducted prior to a large-scale tagging project investigating the movement of approximately 400 fishes from 30 species on offshore coral reefs in the central Red Sea. During this range test we determined the effect of the following factors on transmitter detection efficiency: distance from receiver, time of day, depth, wind, current, moon-phase and temperature. The experiment showed that biological noise is likely to be responsible for a diel pattern of -on average- twice as many detections during the day as during the night. Biological noise appears to be the most important noise source in coral reefs overwhelming the effect of wind-driven noise, which is important in other studies. Detection probability is also heavily influenced by the location of the acoustic sensor within the reef structure. Understanding the effect of environmental factors on transmitter detection probability allowed us to design a more effective receiver array for the large-scale tagging study.

  9. Tag-Split Cache for Efficient GPGPU Cache Utilization

    Energy Technology Data Exchange (ETDEWEB)

    Li, Lingda; Hayes, Ari; Song, Shuaiwen; Zhang, Eddy

    2016-06-01

    Modern GPUs employ cache to improve memory system efficiency. However, large amount of cache space is underutilized due to irregular memory accesses and poor spatial locality which exhibited commonly in GPU applications. Our experiments show that using smaller cache lines could improve cache space utilization, but it also frequently suffers from significant performance loss by introducing large amount of extra cache requests. In this work, we propose a novel cache design named tag-split cache (TSC) that enables fine-grained cache storage to address the problem of cache space underutilization while keeping memory request number unchanged. TSC divides tag into two parts to reduce storage overhead, and it supports multiple cache line replacement in one cycle.

  10. trieFinder: an efficient program for annotating Digital Gene Expression (DGE) tags.

    Science.gov (United States)

    Renaud, Gabriel; LaFave, Matthew C; Liang, Jin; Wolfsberg, Tyra G; Burgess, Shawn M

    2014-10-13

    Quantification of a transcriptional profile is a useful way to evaluate the activity of a cell at a given point in time. Although RNA-Seq has revolutionized transcriptional profiling, the costs of RNA-Seq are still significantly higher than microarrays, and often the depth of data delivered from RNA-Seq is in excess of what is needed for simple transcript quantification. Digital Gene Expression (DGE) is a cost-effective, sequence-based approach for simple transcript quantification: by sequencing one read per molecule of RNA, this technique can be used to efficiently count transcripts while obviating the need for transcript-length normalization and reducing the total numbers of reads necessary for accurate quantification. Here, we present trieFinder, a program specifically designed to rapidly map, parse, and annotate DGE tags of various lengths against cDNA and/or genomic sequence databases. The trieFinder algorithm maps DGE tags in a two-step process. First, it scans FASTA files of RefSeq, UniGene, and genomic DNA sequences to create a database of all tags that can be derived from a predefined restriction site. Next, it compares the experimental DGE tags to this tag database, taking advantage of the fact that the tags are stored as a prefix tree, or "trie", which allows for linear-time searches for exact matches. DGE tags with mismatches are analyzed by recursive calls in the data structure. We find that, in terms of alignment speed, the mapping functionality of trieFinder compares favorably with Bowtie. trieFinder can quickly provide the user an annotation of the DGE tags from three sources simultaneously, simplifying transcript quantification and novel transcript detection, delivering the data in a simple parsed format, obviating the need to post-process the alignment results. trieFinder is available at http://research.nhgri.nih.gov/software/trieFinder/.

  11. Simple and Efficient Purification of Recombinant Proteins Using the Heparin-Binding Affinity Tag.

    Science.gov (United States)

    Jayanthi, Srinivas; Gundampati, Ravi Kumar; Kumar, Thallapuranam Krishnaswamy Suresh

    2017-11-01

    Heparin, a member of the glycosaminoglycan family, is known to interact with more than 400 different types of proteins. For the past few decades, significant progress has been made to understand the molecular details involved in heparin-protein interactions. Based on the structural knowledge available from the FGF1-heparin interaction studies, we have designed a novel heparin-binding peptide (HBP) affinity tag that can be used for the simple, efficient, and cost-effective purification of recombinant proteins of interest. HBP-tagged fusion proteins can be purified by heparin Sepharose affinity chromatography using a simple sodium chloride gradient to elute the bound fusion protein. In addition, owing to the high density of positive charges on the HBP tag, recombinant target proteins are preferably expressed in their soluble forms. The purification of HBP-fusion proteins can also be achieved in the presence of chemical denaturants, including urea. Additionally, polyclonal antibodies raised against the affinity tag can be used to detect HBP-fused target proteins with high sensitivity. © 2017 by John Wiley & Sons, Inc. Copyright © 2017 John Wiley & Sons, Inc.

  12. LOMA: A fast method to generate efficient tagged-random primers despite amplification bias of random PCR on pathogens

    Directory of Open Access Journals (Sweden)

    Lee Wah

    2008-09-01

    Full Text Available Abstract Background Pathogen detection using DNA microarrays has the potential to become a fast and comprehensive diagnostics tool. However, since pathogen detection chips currently utilize random primers rather than specific primers for the RT-PCR step, bias inherent in random PCR amplification becomes a serious problem that causes large inaccuracies in hybridization signals. Results In this paper, we study how the efficiency of random PCR amplification affects hybridization signals. We describe a model that predicts the amplification efficiency of a given random primer on a target viral genome. The prediction allows us to filter false-negative probes of the genome that lie in regions of poor random PCR amplification and improves the accuracy of pathogen detection. Subsequently, we propose LOMA, an algorithm to generate random primers that have good amplification efficiency. Wet-lab validation showed that the generated random primers improve the amplification efficiency significantly. Conclusion The blind use of a random primer with attached universal tag (random-tagged primer in a PCR reaction on a pathogen sample may not lead to a successful amplification. Thus, the design of random-tagged primers is an important consideration when performing PCR.

  13. Spatial-Temporal Event Detection from Geo-Tagged Tweets

    Directory of Open Access Journals (Sweden)

    Yuqian Huang

    2018-04-01

    Full Text Available As one of the most popular social networking services in the world, Twitter allows users to post messages along with their current geographic locations. Such georeferenced or geo-tagged Twitter datasets can benefit location-based services, targeted advertising and geosocial studies. Our study focused on the detection of small-scale spatial-temporal events and their textual content. First, we used Spatial-Temporal Density-Based Spatial Clustering of Applications with Noise (ST-DBSCAN to spatially-temporally cluster the tweets. Then, the word frequencies were summarized for each cluster and the potential topics were modeled by the Latent Dirichlet Allocation (LDA algorithm. Using two years of Twitter data from four college cities in the U.S., we were able to determine the spatial-temporal patterns of two known events, two unknown events and one recurring event, which then were further explored and modeled to identify the semantic content about the events. This paper presents our process and recommendations for both finding event-related tweets as well as understanding the spatial-temporal behaviors and semantic natures of the detected events.

  14. A comparison of tagging methods and their tagging space.

    Science.gov (United States)

    Ke, Xiayi; Miretti, Marcos M; Broxholme, John; Hunt, Sarah; Beck, Stephan; Bentley, David R; Deloukas, Panos; Cardon, Lon R

    2005-09-15

    Single-nucleotide polymorphism (SNP) tagging is widely used as a way of saving genotyping costs in association studies. A number of different tagging methods have been developed to reduce the number of markers to be genotyped while maintaining power for detecting effects on non-assayed SNPs. How the different methods perform in different settings, the degree to which they overlap and share common tags and how they differ are important questions. We investigated these questions by comparing three widely used tagging methods/algorithms--one haplotype r2-based method, one pair-wise r2-based method and one method which was based on haplotype diversity but focused on major haplotypes. Tagging efficiency was defined as the number of genotyped markers divided by the number of tagging SNPs. Tagging effectiveness was defined as the proportion of un-genotyped or 'hidden' SNPs being detected (having a pair-wise or haplotype r2 with a set of tagging SNPs over a threshold, e.g. haplotype r2> or =0.80). The ENCODE regions genotyped on the HapMap CEPH individuals were examined in this study. Tagging effectiveness was generally poor for rare SNPs than for common SNPs, for all three tagging methods. Inclusion of rare SNPs into initial HapMap scheme could enhance the performance of tags on rare hidden SNPs at the expense of increased genotyping cost. At a moderate tagging efficiency, more than 90% of hidden SNPs detected by tagging SNPs selected by one method were also detected by tagging SNPs selected by another method, and this figure could be increased to 100% if tagging efficiency was allowed to drop. These results indicate that the tagging space is highly concordant between different tagging methods, despite the fact that they often involve different sets of tagging SNPs.

  15. "Who Counterfeited My Viagra?" Probabilistic Item Removal Detection via RFID Tag Cooperation

    Directory of Open Access Journals (Sweden)

    Di Pietro Roberto

    2011-01-01

    Full Text Available We leverage RFID tag cooperation to enforce tampering detection. That is, we provide a set of probabilistic protocols that detect the absence of a tag from a system composed of a set of tags and a reader. Our proposals are able to detect which tag and for how long it has been taken away from the system. The grain of the detection can be tuned with respect to the resources available on the tags. Another merit of our solutions is to provide a proof-of-concept that a small level of cooperation among tags can further extend the range of applications RFID can support, possibly opening new veins of research. The proposed protocols fit the resource constraints of the several classes of RFID available on the market. In particular, the memory requirement ranges from few memory slots to a number of memory slots that is proportional to the number of rounds the presence of a tag is going to be checked. Computation is just one hash per round. This fully fledged set of protocols is thought to trade off the detection grain with the resources on the tag: the finer the item removal detection grain, the more resources a protocol requires. A thorough analysis for the removal detection probability is provided. Finally, extensive simulations support the analytical results, showing the viability of the proposed solutions.

  16. An incremental community detection method for social tagging systems using locality-sensitive hashing.

    Science.gov (United States)

    Wu, Zhenyu; Zou, Ming

    2014-10-01

    An increasing number of users interact, collaborate, and share information through social networks. Unprecedented growth in social networks is generating a significant amount of unstructured social data. From such data, distilling communities where users have common interests and tracking variations of users' interests over time are important research tracks in fields such as opinion mining, trend prediction, and personalized services. However, these tasks are extremely difficult considering the highly dynamic characteristics of the data. Existing community detection methods are time consuming, making it difficult to process data in real time. In this paper, dynamic unstructured data is modeled as a stream. Tag assignments stream clustering (TASC), an incremental scalable community detection method, is proposed based on locality-sensitive hashing. Both tags and latent interactions among users are incorporated in the method. In our experiments, the social dynamic behaviors of users are first analyzed. The proposed TASC method is then compared with state-of-the-art clustering methods such as StreamKmeans and incremental k-clique; results indicate that TASC can detect communities more efficiently and effectively. Copyright © 2014 Elsevier Ltd. All rights reserved.

  17. A chimeric affinity tag for efficient expression and chromatographic purification of heterologous proteins from plants

    Directory of Open Access Journals (Sweden)

    Frank eSainsbury

    2016-02-01

    Full Text Available The use of plants as expression hosts for recombinant proteins is an increasingly attractive option for the production of complex and challenging biopharmaceuticals. Tools are needed at present to marry recent developments in high-yielding gene vectors for heterologous expression with routine protein purification techniques. In this study we designed the Cysta-tag, a new purification tag for immobilized metal affinity chromatography (IMAC of plant-made proteins based on the protein-stabilizing fusion partner SlCYS8. We show that the Cysta-tag may be used to rapidly purify proteins under native conditions, and then be removed enzymatically to isolate the protein of interest. We also show that commonly used protease recognition sites for linking purification tags are differentially stable in leaves of the commonly used expression host Nicotiana benthamiana, with those linkers susceptible to cysteine proteases being less stable then serine protease-cleavable linkers. As an example we describe a Cysta-tag experimental scheme for the one-step purification of a clinically useful protein, human α1-antitrypsin, transiently expressed in N. benthamiana. With potential applicability to the variety of chromatography formats commercially available for IMAC-based protein purification, the Cysta-tag provides a convenient means for the efficient and cost-effective purification of recombinant proteins from plant tissues.

  18. Maximum Likelihood Approach for RFID Tag Set Cardinality Estimation with Detection Errors

    DEFF Research Database (Denmark)

    Nguyen, Chuyen T.; Hayashi, Kazunori; Kaneko, Megumi

    2013-01-01

    Abstract Estimation schemes of Radio Frequency IDentification (RFID) tag set cardinality are studied in this paper using Maximum Likelihood (ML) approach. We consider the estimation problem under the model of multiple independent reader sessions with detection errors due to unreliable radio...... is evaluated under dierent system parameters and compared with that of the conventional method via computer simulations assuming flat Rayleigh fading environments and framed-slotted ALOHA based protocol. Keywords RFID tag cardinality estimation maximum likelihood detection error...

  19. Short-chain fluorescent tryptophan tags for on-line detection of functional recombinant proteins

    Directory of Open Access Journals (Sweden)

    Siepert Eva-Maria

    2012-09-01

    Full Text Available Abstract Background Conventional fluorescent proteins, such as GFP, its derivatives and flavin mononucleotide based fluorescent proteins (FbFPs are often used as fusion tags for detecting recombinant proteins during cultivation. These reporter tags are state-of-the-art; however, they have some drawbacks, which can make on-line monitoring challenging. It is discussed in the literature that the large molecular size of proteins of the GFP family may stress the host cell metabolism during production. In addition, fluorophore formation of GFP derivatives is oxygen-dependent resulting in a lag-time between expression and fluorescence detection and the maturation of the protein is suppressed under oxygen-limited conditions. On the contrary, FbFPs are also applicable in an oxygen-limited or even anaerobic environment but are still quite large (58% of the size of GFP. Results As an alternative to common fluorescent tags we developed five novel tags based on clustered tryptophan residues, called W-tags. They are only 5-11% of the size of GFP. Based on the property of tryptophan to fluoresce in absence of oxygen it is reasonable to assume that the functionality of our W-tags is also given under anaerobic conditions. We fused these W-tags to a recombinant protein model, the anti-CD30 receptor single-chain fragment variable antibody (scFv Ki-4(scFv and the anti-MucI single-chain fragment variable M12(scFv. During cultivation in Microtiter plates, the overall tryptophan fluorescence intensity of all cultures was measured on-line for monitoring product formation via the different W-tags. After correlation of the scattered light signal representing biomass concentration and tryptophan fluorescence for the uninduced cultures, the fluorescence originating from the biomass was subtracted from the overall tryptophan signal. The resulting signal, thus, represents the product fluorescence of the tagged and untagged antibody fragments. The product fluorescence signal

  20. Analysis and design of power efficient semi-passive RFID tag

    International Nuclear Information System (INIS)

    Che Wenyi; Guan Shuo; Wang Xiao; Xiong Tingwen; Xi Jingtian; Tan Xi; Yan Na; Min Hao

    2010-01-01

    The analysis and design of a semi-passive radio frequency identification (RFID) tag is presented. By studying the power transmission link of the backscatter RFID system and exploiting a power conversion efficiency model for a multi-stage AC-DC charge pump, the calculation method for semi-passive tag's read range is proposed. According to different read range limitation factors, an intuitive way to define the specifications of tag's power budget and backscatter modulation index is given. A test chip is implemented in SMIC 0.18 μm standard CMOS technology under the guidance of theoretical analysis. The main building blocks are the threshold compensated charge pump and low power wake-up circuit using the power triggering wake-up mode. The proposed semi-passive tag is fully compatible to EPC C1G2 standard. It has a compact chip size of 0.54 mm 2 , and is adaptable to batteries with a 1.2 to 2.4 V output voltage.

  1. Method of remote powering and detecting multiple UWB passive tags in an RFID system

    Science.gov (United States)

    Dowla, Farid U [Castro Valley, CA; Nekoogar, Faranak [San Ramon, CA; Benzel, David M [Livermore, CA; Dallum, Gregory E [Livermore, CA; Spiridon, Alex [Palo Alto, CA

    2012-05-29

    A new Radio Frequency Identification (RFID), tracking, powering apparatus/system and method using coded Ultra-wideband (UWB) signaling is introduced. The proposed hardware and techniques disclosed herein utilize a plurality of passive UWB transponders in a field of an RFID-radar system. The radar system itself enables multiple passive tags to be remotely powered (activated) at about the same time frame via predetermined frequency UWB pulsed formats. Once such tags are in an activated state, an UWB radar transmits specific "interrogating codes" to put predetermined tags in an awakened status. Such predetermined tags can then communicate by a unique "response code" so as to be detected by an UWB system using radar methods.

  2. Tips on improving the efficiency of electrotransfer of target proteins from Phos-tag SDS-PAGE gel.

    Science.gov (United States)

    Kinoshita-Kikuta, Emiko; Kinoshita, Eiji; Matsuda, Ayumi; Koike, Tohru

    2014-11-01

    The sensitivity of Western blotting analysis after Phos-tag SDS-PAGE is occasionally inferior to that after normal (Phos-tag-free) SDS-PAGE under similar experimental conditions, possibly as a result of inefficient electrotransfer from the Phos-tag gel to the blotting membrane. We therefore present tips on improving the efficiency of electrotransfer of proteins in semidry and wet-tank blotting. When model samples containing several standard phosphoproteins were subjected to semidry blotting, their electrotransfer efficiencies after Phos-tag SDS-PAGE were markedly inferior to those of their dephosphorylated counterparts in the same gel. This was ameliorated by immersing the electrophoresed Phos-tag gel in a transfer buffer containing 1 mM EDTA for 30 min before electroblotting. Similarly, phosphoproteomes in crude cell extracts were inefficiently transferred by semidry blotting, but the efficiencies of their electrotransfer were improved by pretreatment with EDTA. In contrast, the efficiencies of wet-tank blotting of the same samples were not dependent on the degree of phosphorylation, and the efficiencies of electrotransfer of all proteins from Phos-tag gels were similar to those from normal gels. In some cases involving the use of a Phos-tag gel, addition of 0.1% w/v of SDS to the transfer buffer significantly improved the electrotransfer. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. Chemical tagging of chlorinated phenols for their facile detection and analysis by NMR spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Valdez, Carlos A. [Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States); Leif, Roald N. [Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

    2015-03-22

    A derivatization method that employs diethyl (bromodifluoromethyl) phosphonate (DBDFP) to efficiently tag the endocrine disruptor pentachlorophenol (PCP) and other chlorinated phenols (CPs) along with their reliable detection and analysis by NMR is presented. The method accomplishes the efficient alkylation of the hydroxyl group in CPs with the difluoromethyl (CF2H) moiety in extremely rapid fashion (5 min), at room temperature and in an environmentally benign manner. The approach proved successful in difluoromethylating a panel of 18 chlorinated phenols, yielding derivatives that displayed unique 1H, 19F NMR spectra allowing for the clear discrimination between isomerically related CPs. Due to its biphasic nature, the derivatization can be applied to both aqueous and organic mixtures where the analysis of CPs is required. Furthermore, the methodology demonstrates that PCP along with other CPs can be selectively derivatized in the presence of other various aliphatic alcohols, underscoring the superiority of the approach over other general derivatization methods that indiscriminately modify all analytes in a given sample. The present work demonstrates the first application of NMR on the qualitative analysis of these highly toxic and environmentally persistent species.

  4. Hirudin as a novel fusion tag for efficient production of lunasin in Escherichia coli.

    Science.gov (United States)

    Tian, Qinghua; Zhang, Ping; Gao, Zhan; Li, Hengli; Bai, Zhengli; Tan, Shuhua

    2017-07-03

    Fusion expression provides an effective means for the biosynthesis of longer peptides in Escherichia coli. However, the commonly used fusion tags are primarily suitable for laboratory scale applications due to the high cost of commercial affinity resins. Herein, a novel approach exploiting hirudin as a multipurpose fusion tag in combination with tobacco etch virus (TEV) protease cleavage has been developed for the efficient and cost-effective production of a 43-amino acid model peptide lunasin in E. coli at preparative scale. A fusion gene which allows for lunasin to be N-terminally fused to the C-terminus of hirudin through a flexible linker comprising a TEV protease cleavage site was designed and cloned in a secretion vector pTASH. By cultivation in a 7-L bioreactor, the fusion protein was excreted into the culture medium at a high yield of ~380 mg/L, which was conveniently recovered and purified by inexpensive HP20 hydrophobic chromatography at a recovery rate of ~80%. After polishing and cleavage with TEV protease, the finally purified lunasin was obtained with ≥95% purity and yield of ~86 mg/L culture medium. Conclusively, this hirudin tagging strategy is powerful in the production of lunasin and could be applicable for the production of other peptides at preparative scale.

  5. Measurement of c-jet tagging efficiency in ATLAS with W+c-jet events

    CERN Document Server

    Lapertosa, Alessandro; The ATLAS collaboration

    2016-01-01

    The correct identication of the jet quark flavour (flavour tagging) is of fundamental importance for hadron collider experiments, such as ATLAS and CMS at the Large Hadron Collider. In particular, it is an important tool for many physics analyses with heavy flavour jets in the final state (b-quarks from top quark decays, H to bb decays and new physics searches). The ATLAS experiment developed a new flavour tagging algorithm for Run II: in this contribution, the measurement of its tagging efficiency performed on a c-jet enriched sample will be presented, using 13 TeV data collected by ATLAS during 2015. A high purity c-jet sample has been selected by reconstructing W+c-jet events, with the W boson reconstructed via its decay into an electron and a neutrino and the c-jet identified via a soft muon stemming from a semileptonic c-hadron decay, exploiting the charge correlation between the electron and the muon.

  6. A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli

    Directory of Open Access Journals (Sweden)

    Jarmander Johan

    2012-09-01

    Full Text Available Abstract Background The discovery of the autotransporter family has provided a mechanism for surface expression of proteins in laboratory strains of Escherichia coli. We have previously reported the use of the AIDA-I autotransport system to express the Salmonella enterica serovar Enteritidis proteins SefA and H:gm. The SefA protein was successfully exposed to the medium, but the orientation of H:gm in the outer membrane could not be determined due to proteolytic cleavage of the N-terminal detection-tag. The goal of the present work was therefore to construct a vector containing elements that facilitates analysis of surface expression, especially for proteins that are sensitive to proteolysis or otherwise difficult to express. Results The surface expression system pAIDA1 was created with two detection tags flanking the passenger protein. Successful expression of SefA and H:gm on the surface of E. coli was confirmed with fluorescently labeled antibodies specific for the N-terminal His6-tag and the C-terminal Myc-tag. While both tags were detected during SefA expression, only the Myc-tag could be detected for H:gm. The negative signal indicates a proteolytic cleavage of this protein that removes the His6-tag facing the medium. Conclusions Expression levels from pAIDA1 were comparable to or higher than those achieved with the formerly used vector. The presence of the Myc- but not of the His6-tag on the cell surface during H:gm expression allowed us to confirm the hypothesis that this fusion protein was present on the surface and oriented towards the cell exterior. Western blot analysis revealed degradation products of the same molecular weight for SefA and H:gm. The size of these fragments suggests that both fusion proteins have been cleaved at a specific site close to the C-terminal end of the passenger. This proteolysis was concluded to take place either in the outer membrane or in the periplasm. Since H:gm was cleaved to a much greater extent

  7. Fluid pipeline leak detection and location with miniature RF tags

    Science.gov (United States)

    McIntyre, Timothy J.

    2017-05-16

    Sensors locate troublesome leaks in pipes or conduits that carry a flowing medium. These sensors, through tailored physical and geometric properties, preferentially seek conduit leaks or breaches due to flow streaming. The sensors can be queried via transceivers outside the conduit or located and interrogated inside by submersible unmanned vehicle to identify and characterize the nature of a leak. The sensors can be functionalized with other capabilities for additional leak and pipeline characterization if needed. Sensors can be recovered from a conduit flow stream and reused for future leak detection activities.

  8. Highly efficient one-step scarless protein tagging by type IIS restriction endonuclease-mediated precision cloning.

    Science.gov (United States)

    Xu, Zhen; Rui, Yan-Ning; Balzeau, Julien; Menezes, Miriam R; Niu, Airu; Hagan, John P; Kim, Dong H

    2017-08-12

    Protein tagging with a wide variety of epitopes and/or fusion partners is used routinely to dissect protein function molecularly. Frequently, the required DNA subcloning is inefficient, especially in cases where multiple constructs are desired for a given protein with unique tags. Additionally, the generated clones have unwanted junction sequences introduced. To add versatile tags into the extracellular domain of the transmembrane protein THSD1, we developed a protein tagging technique that utilizes non-classical type IIS restriction enzymes that recognize non-palindromic DNA sequences and cleave outside of their recognition sites. Our results demonstrate that this method is highly efficient and can precisely fuse any tag into any position of a protein in a scarless manner. Moreover, this method is cost-efficient and adaptable because it uses commercially available type IIS restriction enzymes and is compatible with the traditional cloning system used by many labs. Therefore, precision tagging technology will benefit a number of researchers by providing an alternate method to integrate an array of tags into protein expression constructs. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  9. Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection.

    Science.gov (United States)

    Verma, Vaishali; Kaur, Charanpreet; Grover, Payal; Gupta, Amita; Chaudhary, Vijay K

    2018-01-01

    The high-affinity interaction between biotin and streptavidin has opened avenues for using recombinant proteins with site-specific biotinylation to achieve efficient and directional immobilization. The site-specific biotinylation of proteins carrying a 15 amino acid long Biotin Acceptor Peptide tag (BAP; also known as AviTag) is effected on a specific lysine either by co-expressing the E. coli BirA enzyme in vivo or by using purified recombinant E. coli BirA enzyme in the presence of ATP and biotin in vitro. In this paper, we have designed a T7 promoter-lac operator-based expression vector for rapid and efficient cloning, and high-level cytosolic expression of proteins carrying a C-terminal BAP tag in E. coli with TEV protease cleavable N-terminal deca-histidine tag, useful for initial purification. Furthermore, a robust three-step purification pipeline integrated with well-optimized protocols for TEV protease-based H10 tag removal, and recombinant BirA enzyme-based site-specific in vitro biotinylation is described to obtain highly pure biotinylated proteins. Most importantly, the paper demonstrates superior sensitivities in indirect ELISA with directional and efficient immobilization of biotin-tagged proteins on streptavidin-coated surfaces in comparison to passive immobilization. The use of biotin-tagged proteins through specific immobilization also allows more efficient selection of binders from a phage-displayed naïve antibody library. In addition, for both these applications, specific immobilization requires much less amount of protein as compared to passive immobilization and can be easily multiplexed. The simplified strategy described here for the production of highly pure biotin-tagged proteins will find use in numerous applications, including those, which may require immobilization of multiple proteins simultaneously on a solid surface.

  10. Development of an automated method of detecting stereotyped feeding events in multisensor data from tagged rorqual whales.

    Science.gov (United States)

    Allen, Ann N; Goldbogen, Jeremy A; Friedlaender, Ari S; Calambokidis, John

    2016-10-01

    The introduction of animal-borne, multisensor tags has opened up many opportunities for ecological research, making previously inaccessible species and behaviors observable. The advancement of tag technology and the increasingly widespread use of bio-logging tags are leading to large volumes of sometimes extremely detailed data. With the increasing quantity and duration of tag deployments, a set of tools needs to be developed to aid in facilitating and standardizing the analysis of movement sensor data. Here, we developed an observation-based decision tree method to detect feeding events in data from multisensor movement tags attached to fin whales (Balaenoptera physalus ). Fin whales exhibit an energetically costly and kinematically complex foraging behavior called lunge feeding, an intermittent ram filtration mechanism. Using this automated system, we identified feeding lunges in 19 fin whales tagged with multisensor tags, during a total of over 100 h of continuously sampled data. Using movement sensor and hydrophone data, the automated lunge detector correctly identified an average of 92.8% of all lunges, with a false-positive rate of 9.5%. The strong performance of our automated feeding detector demonstrates an effective, straightforward method of activity identification in animal-borne movement tag data. Our method employs a detection algorithm that utilizes a hierarchy of simple thresholds based on knowledge of observed features of feeding behavior, a technique that is readily modifiable to fit a variety of species and behaviors. Using automated methods to detect behavioral events in tag records will significantly decrease data analysis time and aid in standardizing analysis methods, crucial objectives with the rapidly increasing quantity and variety of on-animal tag data. Furthermore, our results have implications for next-generation tag design, especially long-term tags that can be outfitted with on-board processing algorithms that automatically detect

  11. His-tag ELISA for the detection of humoral tumor-specific immunity

    Directory of Open Access Journals (Sweden)

    Disis Mary L

    2008-05-01

    Full Text Available Abstract Background The application of high throughput molecular techniques such as SEREX are resulting in the identification of a multitude of tumor associated antigens. As newly identified antigens are incorporated into a variety of clinical trials, standardization of immunologic monitoring methods becomes increasingly important. We questioned whether mammalian cell expression of a histadine-linked human protein could be used to produce antigen suitable for detecting tumor-specific humoral immunity and whether such an assay could be amenable to standardization for clinical use. Methods We designed a his-tagged capture ELISA based on lysate from genetically engineered CHO cells for detection of antibodies to insulin-like growth factor binding protein 2, a novel tumor antigen. We performed technical and preliminary clinical validation studies, including comparison to a standard indirect ELISA based on commercially prepared recombinant antigen. Results The his-tagged capture ELISA could be standardized. Precision experiments resulted in CVs 2 values of 0.99. In comparison to Western blot analysis, his-tag and indirect ELISA accurately identified 88% and 93% of samples, respectively. Sample concordance between capture and indirect assays was highly significant (p = 0.003. Furthermore, significantly greater levels of IGFBP-2 antibody immunity were found in cancer patients compared to normal controls (p = 0.008. Conclusion A genetically engineered cell lysate based ELISA can be amenable to standardization and can detect increased levels of antibody immunity to tumor-associated antigen in cancer patients compared to non tumor-bearing healthy controls.

  12. Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice

    Science.gov (United States)

    de Boer, Ernie; Rodriguez, Patrick; Bonte, Edgar; Krijgsveld, Jeroen; Katsantoni, Eleni; Heck, Albert; Grosveld, Frank; Strouboulis, John

    2003-06-01

    Proteomic approaches require simple and efficient protein purification methodologies that are amenable to high throughput. Biotinylation is an attractive approach for protein complex purification due to the very high affinity of avidin/streptavidin for biotinylated templates. Here, we describe an approach for the single-step purification of transcription factor complex(es) based on specific in vivo biotinylation. We expressed the bacterial BirA biotin ligase in mammalian cells and demonstrated very efficient biotinylation of a hematopoietic transcription factor bearing a small (23-aa) artificial peptide tag. Biotinylation of the tagged transcription factor altered neither the factor's protein interactions or DNA binding properties in vivo nor its subnuclear distribution. Using this approach, we isolated the biotin-tagged transcription factor and at least one other known interacting protein from crude nuclear extracts by direct binding to streptavidin beads. Finally, this method works efficiently in transgenic mice, thus raising the prospect of using biotinylation tagging in protein complex purification directly from animal tissues. Therefore, BirA-mediated biotinylation of tagged proteins provides the basis for the single-step purification of proteins from mammalian cells.

  13. Separation and detection of large phosphoproteins using Phos-tag SDS-PAGE.

    Science.gov (United States)

    Kinoshita, Eiji; Kinoshita-Kikuta, Emiko; Koike, Tohru

    2009-01-01

    We provide a standard phosphate-affinity SDS-PAGE (Mn(2+)-Phos-tag SDS-PAGE) protocol, in which Phos-tag is used to analyze large phosphoproteins with molecular masses of more than 200 kDa. A previous protocol required a long electrophoresis time of 12 h for separation of phosphoisotypes of large proteins ( approximately 150 kDa). This protocol, which uses a 3% (wt/vol) polyacrylamide gel strengthened with 0.5% (wt/vol) agarose, permits the separation of protein phosphoisotypes larger than 200 kDa within 2 h. In subsequent immunoblotting, phosphoisotypes of high-molecular-mass proteins, such as mammalian target of rapamycin (289 kDa), ataxia telangiectasia-mutated kinase (350 kDa) and p53-binding protein 1 (213 kDa), can be clearly detected as up-shifted migration bands on the improved Mn(2+)-Phos-tag SDS-PAGE gel. The procedure from the beginning of gel preparation to the end of electrophoresis requires about 4 h in this protocol.

  14. SU-E-I-65: Estimation of Tagging Efficiency in Pseudo-Continuous Arterial Spin Labeling (pCASL) MRI

    Energy Technology Data Exchange (ETDEWEB)

    Jen, M [Chang Gung University, Taoyuan City, Taiwan (China); Yan, F; Tseng, Y; Chen, C [Taipei Medical University - Shuang Ho Hospital, Ministry of Health and Welf, New Taipei City, Taiwan (China); Lin, C [GE Healthcare, Taiwan (China); GE Healthcare China, Beijing (China); Liu, H [UT MD Anderson Cancer Center, Houston, TX (United States)

    2015-06-15

    Purpose: pCASL was recommended as a potent approach for absolute cerebral blood flow (CBF) quantification in clinical practice. However, uncertainties of tagging efficiency in pCASL remain an issue. This study aimed to estimate tagging efficiency by using short quantitative pulsed ASL scan (FAIR-QUIPSSII) and compare resultant CBF values with those calibrated by using 2D Phase Contrast (PC) MRI. Methods: Fourteen normal volunteers participated in this study. All images, including whole brain (WB) pCASL, WB FAIR-QUIPSSII and single-slice 2D PC, were collected on a 3T clinical MRI scanner with a 8-channel head coil. DeltaM map was calculated by averaging the subtraction of tag/control pairs in pCASL and FAIR-QUIPSSII images and used for CBF calculation. Tagging efficiency was then calculated by the ratio of mean gray matter CBF obtained from pCASL and FAIR-QUIPSSII. For comparison, tagging efficiency was also estimated with 2D PC, a previously established method, by contrast WB CBF in pCASL and 2D PC. Feasibility of estimation from a short FAIR-QUIPSSII scan was evaluated by number of averages required for obtaining a stable deltaM value. Setting deltaM calculated by maximum number of averaging (50 pairs) as reference, stable results were defined within ±10% variation. Results: Tagging efficiencies obtained by 2D PC MRI (0.732±0.092) were significantly lower than which obtained by FAIRQUIPPSSII (0.846±0.097) (P<0.05). Feasibility results revealed that four pairs of images in FAIR-QUIPPSSII scan were sufficient to obtain a robust calibration of less than 10% differences from using 50 pairs. Conclusion: This study found that reliable estimation of tagging efficiency could be obtained by a few pairs of FAIR-QUIPSSII images, which suggested that calibration scan in a short duration (within 30s) was feasible. Considering recent reports concerning variability of PC MRI-based calibration, this study proposed an effective alternative for CBF quantification with pCASL.

  15. Simultaneous electrochemical detection of multiple tumor markers using metal ions tagged immunocolloidal gold.

    Science.gov (United States)

    Xu, Teng; Jia, Xinle; Chen, Xia; Ma, Zhanfang

    2014-06-15

    In this work, a sandwich-format electrochemical immunosensor has been fabricated in the aim of simultaneous detection of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) using metal ions tagged immunocolloidal gold nanocomposites as signal tags. The capture anti-CEA and anti-AFP were immobilized onto the chitosan-Au nanoparticles (CHIT-AuNPs) membrane modified glassy carbon electrode through glutaraldehyde (GA). The metal ion labels could be detected directly through differential pulse voltammetry (DPV) without metal pre-concentration, and the distinct voltammetric peaks had a close relationship with each sandwich-type immunoreaction. Under the optimized conditions, the multiplexed immunoassay exhibited good sensitivity and selectivity for the simultaneous determination of CEA and AFP with linear ranges of 0.01-50 ng mL(-1). The detection limits for CEA and AFP are 4.6 pg mL(-1) and 3.1 pg mL(-1), respectively. The method was successfully applied for the determination of AFP and CEA levels in clinical serum samples, and the results were in good agreement with standard enzyme linked immunosorbent assay (ELISA). This approach gives a promising simple and sensitive immunoassay strategy for clinical applications. Copyright © 2014 Elsevier B.V. All rights reserved.

  16. Myocardial deformation from tagged MRI in hypertrophic cardiomyopathy using an efficient registration strategy

    Science.gov (United States)

    Piella, G.; De Craene, M.; Oubel, E.; Larrabide, I.; Huguet, M.; Bijnens, B. H.; Frangi, A. F.

    2009-02-01

    This paper combines different parallelization strategies for speeding up motion and deformation computation by non-rigid registration of a sequence of images. The registration is performed in a two-level acceleration approach: (1) parallelization of each registration process using MPI and/or threads, and (2) distribution of the sequential registrations over a cluster. On a 24-node double quad-core Intel Xeon (2.66 GHz CPU, 16 GB RAM) cluster, the method is demonstrated to efficiently compute the deformation of a cardiac sequence reducing the computation time from more than 3 hours to a couple of minutes (for low downsampled images). It is shown that the distribution of the sequential registrations over the cluster together with the parallelization of each pairwise registration by multithreading lowers the computation time towards values compatible with clinical requirements (a few minutes per patient). The combination of MPI and multithreading is only advantageous for large input data sizes. Performances are assessed for the specific scenario of aligning cardiac sequences of taggedMagnetic Resonance (tMR) images, with the aim of comparing strain in healthy subjects and hypertrophic cardiomyopathy (HCM) patients. In particular, we compared the distribution of systolic strain in both populations. On average, HCM patients showed lower average values of strain with larger deviation due to the coexistence of regions with impaired deformation and regions with normal deformation.

  17. Studies on nitrogen use efficiency in turmeric using 15N tagged urea

    International Nuclear Information System (INIS)

    Jagadeeswaran, R.; Arulmozhiselvan, K.; Govindaswamy, M.; Murugappan, V.

    2004-01-01

    A greenhouse experiment was conducted with turmeric in order to understand the partitioning of N between shoot and rhizome and to study the N use efficiency using 15 N tagged urea. The experiment was conducted in a completely randomized block design with seven treatments consisting of whole N application as basal and split applications ranging from two to six, besides a control. The result indicated that N derived from 15 N urea (Ndff) increased with number of split application of N up to four splits in turmeric shoot and up to three splits in turmeric rhizome, both at 180 days as well as at harvest stage. Increasing the splits beyond this declined Ndff, which indicated that five and more number of splits of N would not help to increase the uptake of applied N. Thus, the present study clearly revealed that in turmeric, application of N in three splits would be optimal in increasing the Ndff in rhizome. Fertilizer N balance calculations clearly indicated that the recovery of N in turmeric was high in favour of four splits at 180 days growth stage (19.46 per cent) as well as at harvest (30.76 per cent). (author)

  18. Efficient protein production method for NMR using soluble protein tags with cold shock expression vector

    International Nuclear Information System (INIS)

    Hayashi, Kokoro; Kojima, Chojiro

    2010-01-01

    The E. coli protein expression system is one of the most useful methods employed for NMR sample preparation. However, the production of some recombinant proteins in E. coli is often hampered by difficulties such as low expression level and low solubility. To address these problems, a modified cold-shock expression system containing a glutathione S-transferase (GST) tag, the pCold-GST system, was investigated. The pCold-GST system successfully expressed 9 out of 10 proteins that otherwise could not be expressed using a conventional E. coli expression system. Here, we applied the pCold-GST system to 84 proteins and 78 proteins were successfully expressed in the soluble fraction. Three other cold-shock expression systems containing a maltose binding protein tag (pCold-MBP), protein G B1 domain tag (pCold-GB1) or thioredoxin tag (pCold-Trx) were also developed to improve the yield. Additionally, we show that a C-terminal proline tag, which is invisible in 1 H- 15 N HSQC spectra, inhibits protein degradation and increases the final yield of unstable proteins. The purified proteins were amenable to NMR analyses. These data suggest that pCold expression systems combined with soluble protein tags can be utilized to improve the expression and purification of various proteins for NMR analysis.

  19. A Novel Method for Mining SaaS Software Tag via Community Detection in Software Services Network

    Science.gov (United States)

    Qin, Li; Li, Bing; Pan, Wei-Feng; Peng, Tao

    The number of online software services based on SaaS paradigm is increasing. However, users usually find it hard to get the exact software services they need. At present, tags are widely used to annotate specific software services and also to facilitate the searching of them. Currently these tags are arbitrary and ambiguous since mostly of them are generated manually by service developers. This paper proposes a method for mining tags from the help documents of software services. By extracting terms from the help documents and calculating the similarity between the terms, we construct a software similarity network where nodes represent software services, edges denote the similarity relationship between software services, and the weights of the edges are the similarity degrees. The hierarchical clustering algorithm is used for community detection in this software similarity network. At the final stage, tags are mined for each of the communities and stored as ontology.

  20. Detecting host-parasitoid interactions in an invasive Lepidopteran using nested tagging DNA metabarcoding.

    Science.gov (United States)

    Kitson, James J N; Hahn, Christoph; Sands, Richard J; Straw, Nigel A; Evans, Darren M; Lunt, David H

    2018-02-27

    Determining the host-parasitoid interactions and parasitism rates for invasive species entering novel environments is an important first step in assessing potential routes for biocontrol and integrated pest management. Conventional insect rearing techniques followed by taxonomic identification are widely used to obtain such data, but this can be time-consuming and prone to biases. Here, we present a next-generation sequencing approach for use in ecological studies which allows for individual-level metadata tracking of large numbers of invertebrate samples through the use of hierarchically organised molecular identification tags. We demonstrate its utility using a sample data set examining both species identity and levels of parasitism in late larval stages of the oak processionary moth (Thaumetopoea processionea-Linn. 1758), an invasive species recently established in the United Kingdom. Overall, we find that there are two main species exploiting the late larval stages of oak processionary moth in the United Kingdom with the main parasitoid (Carcelia iliaca-Ratzeburg, 1840) parasitising 45.7% of caterpillars, while a rare secondary parasitoid (Compsilura concinnata-Meigen, 1824) was also detected in 0.4% of caterpillars. Using this approach on all life stages of the oak processionary moth may demonstrate additional parasitoid diversity. We discuss the wider potential of nested tagging DNA metabarcoding for constructing large, highly resolved species interaction networks. © 2018 John Wiley & Sons Ltd.

  1. Facile fabrication of nickel immobilized on magnetic nanoparticles as an efficient affinity adsorbent for purification of his-tagged protein.

    Science.gov (United States)

    Rashid, Zahra; Naeimi, Hossein; Zarnani, Amir-Hassan; Mohammadi, Fereshteh; Ghahremanzadeh, Ramin

    2017-11-01

    In the present research, an efficient, convenient, and inexpensive method for the one-pot synthesis of Fe 3 O 4 @Histidine is developed. Histidine is readily loaded on magnetic nanoparticles by one step and simple method without any supplemental linkers. In the structure of Fe 3 O 4 @Histidine, histidine covalently immobilized on the surface of Fe 3 O 4 , magnetic nanoparticles are able to trap Ni 2+ ions through a strong interaction between nickel and histidines in protein tag. Two coordination sites of nickel are occupied with ligand on the surface of magnetic nanoparticles and four coordination sites have been remained that these sites will be occupied with histidine tag of recombinant protein A. The functionalized nanoparticles were spherical and well separated with an average diameter around 30nm. The obtained magnetic nanoparticles have a saturation magnetization of about 54emu/g. Fe 3 O 4 @Histidine-Ni was used to enrich and purify 6×histidine-tagged recombinant protein-A directly from the mixture of lysed cells. It has been found that Ni(II)-immobilized Fe 3 O 4 @Histidine magnetic nanoparticles present negligible nonspecific protein adsorption and high His-tag protein binding capacity The average binding capacity (MW 42k Da), is 700±25μg·mg -1 (protein/Fe 3 O 4 @Histidine-Ni). Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Optimal Detection Range of RFID Tag for RFID-based Positioning System Using the k-NN Algorithm

    Directory of Open Access Journals (Sweden)

    Joon Heo

    2009-06-01

    Full Text Available Positioning technology to track a moving object is an important and essential component of ubiquitous computing environments and applications. An RFID-based positioning system using the k-nearest neighbor (k-NN algorithm can determine the position of a moving reader from observed reference data. In this study, the optimal detection range of an RFID-based positioning system was determined on the principle that tag spacing can be derived from the detection range. It was assumed that reference tags without signal strength information are regularly distributed in 1-, 2- and 3-dimensional spaces. The optimal detection range was determined, through analytical and numerical approaches, to be 125% of the tag-spacing distance in 1-dimensional space. Through numerical approaches, the range was 134% in 2-dimensional space, 143% in 3-dimensional space.

  3. Undesirable charge-enhancement of isobaric tagged phosphopeptides leads to reduced identification efficiency

    DEFF Research Database (Denmark)

    Thingholm, Tine E; Palmisano, Giuseppe; Kjeldsen, Frank

    2010-01-01

    labeling of proteins and peptides for in vitro cell culture systems (stable isotope labeling using amino acids in cell culture, SILAC) or isobaric peptide labels such as isobaric tags for relative and absolute quantitation (iTRAQ) and tandem mass tags (TMT) for both in vitro and in vivo systems...... identifications observed for large cell- or tissue-based data sets from labeled and nonlabeled peptide mixtures. Ammonia vapor sprayed perpendicular to the electrospray needle during ionization resulted in an overall decrease in the average charge states and a concomitant increase in phosphopeptide...

  4. Detection, Identification, Location, and Remote Sensing using SAW RFID Sensor Tags

    Science.gov (United States)

    Barton, Richard J.

    2009-01-01

    In this presentation, we will consider the problem of simultaneous detection, identification, location estimation, and remote sensing for multiple objects. In particular, we will describe the design and testing of a wireless system capable of simultaneously detecting the presence of multiple objects, identifying each object, and acquiring both a low-resolution estimate of location and a high-resolution estimate of temperature for each object based on wireless interrogation of passive surface acoustic wave (SAW) radiofrequency identification (RFID) sensor tags affixed to each object. The system is being studied for application on the lunar surface as well as for terrestrial remote sensing applications such as pre-launch monitoring and testing of spacecraft on the launch pad and monitoring of test facilities. The system utilizes a digitally beam-formed planar receiving antenna array to extend range and provide direction-of-arrival information coupled with an approximate maximum-likelihood signal processing algorithm to provide near-optimal estimation of both range and temperature. The system is capable of forming a large number of beams within the field of view and resolving the information from several tags within each beam. The combination of both spatial and waveform discrimination provides the capability to track and monitor telemetry from a large number of objects appearing simultaneously within the field of view of the receiving array. In the presentation, we will summarize the system design and illustrate several aspects of the operational characteristics and signal structure. We will examine the theoretical performance characteristics of the system and compare the theoretical results with results obtained from experiments in both controlled laboratory environments and in the field.

  5. Grizzly Bear Noninvasive Genetic Tagging Surveys: Estimating the Magnitude of Missed Detections.

    Science.gov (United States)

    Fisher, Jason T; Heim, Nicole; Code, Sandra; Paczkowski, John

    2016-01-01

    Sound wildlife conservation decisions require sound information, and scientists increasingly rely on remotely collected data over large spatial scales, such as noninvasive genetic tagging (NGT). Grizzly bears (Ursus arctos), for example, are difficult to study at population scales except with noninvasive data, and NGT via hair trapping informs management over much of grizzly bears' range. Considerable statistical effort has gone into estimating sources of heterogeneity, but detection error-arising when a visiting bear fails to leave a hair sample-has not been independently estimated. We used camera traps to survey grizzly bear occurrence at fixed hair traps and multi-method hierarchical occupancy models to estimate the probability that a visiting bear actually leaves a hair sample with viable DNA. We surveyed grizzly bears via hair trapping and camera trapping for 8 monthly surveys at 50 (2012) and 76 (2013) sites in the Rocky Mountains of Alberta, Canada. We used multi-method occupancy models to estimate site occupancy, probability of detection, and conditional occupancy at a hair trap. We tested the prediction that detection error in NGT studies could be induced by temporal variability within season, leading to underestimation of occupancy. NGT via hair trapping consistently underestimated grizzly bear occupancy at a site when compared to camera trapping. At best occupancy was underestimated by 50%; at worst, by 95%. Probability of false absence was reduced through successive surveys, but this mainly accounts for error imparted by movement among repeated surveys, not necessarily missed detections by extant bears. The implications of missed detections and biased occupancy estimates for density estimation-which form the crux of management plans-require consideration. We suggest hair-trap NGT studies should estimate and correct detection error using independent survey methods such as cameras, to ensure the reliability of the data upon which species management and

  6. Grizzly Bear Noninvasive Genetic Tagging Surveys: Estimating the Magnitude of Missed Detections

    Science.gov (United States)

    Fisher, Jason T.; Heim, Nicole; Code, Sandra; Paczkowski, John

    2016-01-01

    Sound wildlife conservation decisions require sound information, and scientists increasingly rely on remotely collected data over large spatial scales, such as noninvasive genetic tagging (NGT). Grizzly bears (Ursus arctos), for example, are difficult to study at population scales except with noninvasive data, and NGT via hair trapping informs management over much of grizzly bears’ range. Considerable statistical effort has gone into estimating sources of heterogeneity, but detection error–arising when a visiting bear fails to leave a hair sample–has not been independently estimated. We used camera traps to survey grizzly bear occurrence at fixed hair traps and multi-method hierarchical occupancy models to estimate the probability that a visiting bear actually leaves a hair sample with viable DNA. We surveyed grizzly bears via hair trapping and camera trapping for 8 monthly surveys at 50 (2012) and 76 (2013) sites in the Rocky Mountains of Alberta, Canada. We used multi-method occupancy models to estimate site occupancy, probability of detection, and conditional occupancy at a hair trap. We tested the prediction that detection error in NGT studies could be induced by temporal variability within season, leading to underestimation of occupancy. NGT via hair trapping consistently underestimated grizzly bear occupancy at a site when compared to camera trapping. At best occupancy was underestimated by 50%; at worst, by 95%. Probability of false absence was reduced through successive surveys, but this mainly accounts for error imparted by movement among repeated surveys, not necessarily missed detections by extant bears. The implications of missed detections and biased occupancy estimates for density estimation–which form the crux of management plans–require consideration. We suggest hair-trap NGT studies should estimate and correct detection error using independent survey methods such as cameras, to ensure the reliability of the data upon which species

  7. Grizzly Bear Noninvasive Genetic Tagging Surveys: Estimating the Magnitude of Missed Detections.

    Directory of Open Access Journals (Sweden)

    Jason T Fisher

    Full Text Available Sound wildlife conservation decisions require sound information, and scientists increasingly rely on remotely collected data over large spatial scales, such as noninvasive genetic tagging (NGT. Grizzly bears (Ursus arctos, for example, are difficult to study at population scales except with noninvasive data, and NGT via hair trapping informs management over much of grizzly bears' range. Considerable statistical effort has gone into estimating sources of heterogeneity, but detection error-arising when a visiting bear fails to leave a hair sample-has not been independently estimated. We used camera traps to survey grizzly bear occurrence at fixed hair traps and multi-method hierarchical occupancy models to estimate the probability that a visiting bear actually leaves a hair sample with viable DNA. We surveyed grizzly bears via hair trapping and camera trapping for 8 monthly surveys at 50 (2012 and 76 (2013 sites in the Rocky Mountains of Alberta, Canada. We used multi-method occupancy models to estimate site occupancy, probability of detection, and conditional occupancy at a hair trap. We tested the prediction that detection error in NGT studies could be induced by temporal variability within season, leading to underestimation of occupancy. NGT via hair trapping consistently underestimated grizzly bear occupancy at a site when compared to camera trapping. At best occupancy was underestimated by 50%; at worst, by 95%. Probability of false absence was reduced through successive surveys, but this mainly accounts for error imparted by movement among repeated surveys, not necessarily missed detections by extant bears. The implications of missed detections and biased occupancy estimates for density estimation-which form the crux of management plans-require consideration. We suggest hair-trap NGT studies should estimate and correct detection error using independent survey methods such as cameras, to ensure the reliability of the data upon which species

  8. A Pre-Detection Based Anti-Collision Algorithm with Adjustable Slot Size Scheme for Tag Identification

    Directory of Open Access Journals (Sweden)

    Chiu-Kuo LIANG

    2015-06-01

    Full Text Available One of the research areas in RFID systems is a tag anti-collision protocol; how to reduce identification time with a given number of tags in the field of an RFID reader. There are two types of tag anti-collision protocols for RFID systems: tree based algorithms and slotted aloha based algorithms. Many anti-collision algorithms have been proposed in recent years, especially in tree based protocols. However, there still have challenges on enhancing the system throughput and stability due to the underlying technologies had faced different limitation in system performance when network density is high. Particularly, the tree based protocols had faced the long identification delay. Recently, a Hybrid Hyper Query Tree (H2QT protocol, which is a tree based approach, was proposed and aiming to speedup tag identification in large scale RFID systems. The main idea of H2QT is to track the tag response and try to predict the distribution of tag IDs in order to reduce collisions. In this paper, we propose a pre-detection tree based algorithm, called the Adaptive Pre-Detection Broadcasting Query Tree algorithm (APDBQT, to avoid those unnecessary queries. Our proposed APDBQT protocol can reduce not only the collisions but the idle cycles as well by using pre-detection scheme and adjustable slot size mechanism. The simulation results show that our proposed technique provides superior performance in high density environments. It is shown that the APDBQT is effective in terms of increasing system throughput and minimizing identification delay.

  9. Detection and Tracking of a Novel Genetically Tagged Biological Simulant in the Environment

    Science.gov (United States)

    Emanuel, Peter A.; Buckley, Patricia E.; Sutton, Tiffany A.; Edmonds, Jason M.; Bailey, Andrew M.; Rivers, Bryan A.; Kim, Michael H.; Ginley, William J.; Keiser, Christopher C.; Doherty, Robert W.; Kragl, F. Joseph; Narayanan, Fiona E.; Katoski, Sarah E.; Paikoff, Sari; Leppert, Samuel P.; Strawbridge, John B.; VanReenen, Daniel R.; Biberos, Sally S.; Moore, Douglas; Phillips, Douglas W.; Mingioni, Lisa R.; Melles, Ogba; Ondercin, Daniel G.; Hirsh, Beth; Bieschke, Kendall M.; Harris, Crystal L.; Omberg, Kristin M.; Rastogi, Vipin K.; Van Cuyk, Sheila

    2012-01-01

    A variant of Bacillus thuringiensis subsp. kurstaki containing a single, stable copy of a uniquely amplifiable DNA oligomer integrated into the genome for tracking the fate of biological agents in the environment was developed. The use of genetically tagged spores overcomes the ambiguity of discerning the test material from pre-existing environmental microflora or from previously released background material. In this study, we demonstrate the utility of the genetically “barcoded” simulant in a controlled indoor setting and in an outdoor release. In an ambient breeze tunnel test, spores deposited on tiles were reaerosolized and detected by real-time PCR at distances of 30 m from the point of deposition. Real-time PCR signals were inversely correlated with distance from the seeded tiles. An outdoor release of powdered spore simulant at Aberdeen Proving Ground, Edgewood, MD, was monitored from a distance by a light detection and ranging (LIDAR) laser. Over a 2-week period, an array of air sampling units collected samples were analyzed for the presence of viable spores and using barcode-specific real-time PCR assays. Barcoded B. thuringiensis subsp. kurstaki spores were unambiguously identified on the day of the release, and viable material was recovered in a pattern consistent with the cloud track predicted by prevailing winds and by data tracks provided by the LIDAR system. Finally, the real-time PCR assays successfully differentiated barcoded B. thuringiensis subsp. kurstaki spores from wild-type spores under field conditions. PMID:23001670

  10. CP5 system, for simple and highly efficient protein purification with a C-terminal designed mini tag.

    Directory of Open Access Journals (Sweden)

    Hiroyuki Takeda

    Full Text Available There are many strategies to purify recombinant proteins of interest, and affinity purification utilizing monoclonal antibody that targets a linear epitope sequence is one of the essential techniques used in current biochemistry and structural biology. Here we introduce a new protein purification system using a very short CP5 tag. First, we selected anti-dopamine receptor D1 (DRD1 rabbit monoclonal antibody clone Ra62 (Ra62 antibody as capture antibody, and identified its minimal epitope sequence as a 5-amino-acid sequence at C-terminal of DRD1 (GQHPT-COOH, D1CE sequence. We found that single amino acid substitution in D1CE sequence (GQHVT-COOH increased dissociation rate up to 10-fold, and named the designed epitope sequence CP5 tag. Using Ra62 antibody and 2 peptides with different affinity, we developed a new affinity protein purification method, CP5 system. Ra62 antibody quickly captures CP5-tagged target protein, and captured CP5-tagged protein was eluted by competing with higher affinity D1CE peptide. By taking the difference of the affinity between D1CE and CP5, sharp elution under mild condition was achieved. Using CP5 system, we successfully purified deubiquitinase CYLD and E3 ubiquitin ligase MARCH3, and detected their catalytic activity. As to G protein-coupled receptors (GPCRs, 9 out of 12 cell-free synthesized ones were purified, demonstrating its purification capability of integral membrane proteins. CP5 tagged CHRM2 expressed by baculovirus-insect cell was also successfully purified by CP5 system. CP5 system offers several distinct advantages in addition to its specificity and elution performance. CP5 tag is easy to construct and handle because of its short length, which has less effect on protein characters. Mild elution of CP5 system is particulaly suitable for preparing delicate proteins such as enzymes and membrane proteins. Our data demonstrate that CP5 system provides a new promising option in protein sample preparation with

  11. Fully Printed Flexible Single-Chip RFID Tag with Light Detection Capabilities

    Directory of Open Access Journals (Sweden)

    Aniello Falco

    2017-03-01

    Full Text Available A printed passive radiofrequency identification (RFID tag in the ultra-high frequency band for light and temperature monitoring is presented. The whole tag has been manufactured by printing techniques on a flexible substrate. Antenna and interconnects are realized with silver nanoparticles via inkjet printing. A sprayed photodetector performs the light monitoring, whereas temperature measurement comes from an in-built sensor in the silicon RFID chip. One of the advantages of this system is the digital read-out and transmission of the sensors information on the RFID tag that ensures reliability. Furthermore, the use of printing techniques allows large-scale manufacturing and the direct fabrication of the tag on the desired surface. This work proves for the first time the feasibility of the embedment of large-scale organic photodetectors onto inkjet printed RFID tags. Here, we solve the problem of integration of different manufacturing techniques to develop an optimal final sensor system.

  12. Efficient Collision Detection in a Simulated Hydrocyclone

    NARCIS (Netherlands)

    van Eijkeren, D.F.; Krebs, T.; Hoeijmakers, Hendrik Willem Marie

    2015-01-01

    Hydrocyclones enhance oil–water separation efficiency compared to conventional separation methods. An efficient collision detection scheme with Np ln Np dependency on the number of particles is proposed. The scheme is developed to investigate the importance of particle–particle interaction for flow

  13. Detection efficiency of the multiple muon catalysis

    International Nuclear Information System (INIS)

    Fil'chenkov, V.V.

    2000-01-01

    This work is devoted to further development of the experimental method successfully used at JINR (Dubna) for the muon-catalyzed fusion (MCF) study. Influence of the pile up effects on the MCF product detection efficiency is considered. It is shown that the efficiency slightly depends on the MCF multiplicity

  14. Violation of local realism versus detection efficiency

    International Nuclear Information System (INIS)

    Massar, Serge; Pironio, Stefano

    2003-01-01

    We put bounds on the minimum detection efficiency necessary to violate local realism in Bell experiments. These bounds depend on simple parameters like the number of measurement settings or the dimensionality of the entangled quantum state. We derive them by constructing explicit local hidden variable models which reproduce the quantum correlations for sufficiently small detectors efficiency

  15. Trace detection of specific viable bacteria using tetracysteine-tagged bacteriophages.

    Science.gov (United States)

    Wu, Lina; Luan, Tian; Yang, Xiaoting; Wang, Shuo; Zheng, Yan; Huang, Tianxun; Zhu, Shaobin; Yan, Xiaomei

    2014-01-07

    Advanced methods are urgently needed to determine the identity and viability of trace amounts of pathogenic bacteria in a short time. Existing approaches either fall short in the accurate assessment of microbial viability or lack specificity in bacterial identification. Bacteriophages (or phages for short) are viruses that exclusively infect bacterial host cells with high specificity. As phages infect and replicate only in living bacterial hosts, here we exploit the strategy of using tetracysteine (TC)-tagged phage in combination with biarsenical dye to the discriminative detection of viable target bacteria from dead target cells and other viable but nontarget bacterial cells. Using recombinant M13KE-TC phage and Escherichia coli ER2738 as a model system, distinct differentiation between individual viable target cells from dead target cells was demonstrated by flow cytometry and fluorescence microscopy. As few as 1% viable E. coli ER2738 can be accurately quantified in a mix with dead E. coli ER2738 by flow cytometry. With fluorescence microscopic measurement, specific detection of as rare as 1 cfu/mL original viable target bacteria was achieved in the presence of a large excess of dead target cells and other viable but nontarget bacterial cells in 40 mL artificially contaminated drinking water sample in less than 3 h. This TC-phage-FlAsH approach is sensitive, specific, rapid, and simple, and thus shows great potential in water safety monitoring, health surveillance, and clinical diagnosis of which trace detection and identification of viable bacterial pathogens is highly demanded.

  16. Specific, sensitive, high-resolution detection of protein molecules in eukaryotic cells using metal-tagging transmission electron microscopy

    Science.gov (United States)

    Risco, Cristina; Sanmartín-Conesa, Eva; Tzeng, Wen-Pin; Frey, Teryl K.; Seybold, Volker; de Groot, Raoul J.

    2012-01-01

    Summary More than any other methodology, transmission electron microscopy (TEM) has contributed to our understanding of the architecture and organization of cells. With current detection limits approaching atomic resolution, it will ultimately become possible to ultrastructurally image intracellular macromolecular assemblies in situ. Presently, however, methods to unambiguously identify proteins within the crowded environment of the cell’s interior are lagging behind. We describe a novel approach, metal-tagging TEM (METTEM) that allows detection of intracellular proteins in mammalian cells with high specificity, exceptional sensitivity and at molecular scale resolution. In live cells treated with gold salts, proteins bearing a small metal-binding tag will form 1-nm gold nanoclusters, readily detectable in electron micrographs. The applicability and strength of METTEM is demonstrated by a study of Rubella virus replicase and capsid proteins, which revealed virus-induced cell structures not seen before. PMID:22579245

  17. Highly parallel and short-acting amplification with locus-specific primers to detect single nucleotide polymorphisms by the DigiTag2 assay.

    Directory of Open Access Journals (Sweden)

    Nao Nishida

    Full Text Available The DigiTag2 assay enables analysis of a set of 96 SNPs using Kapa 2GFast HotStart DNA polymerase with a new protocol that has a total running time of about 7 hours, which is 6 hours shorter than the previous protocol. Quality parameters (conversion rate, call rate, reproducibility and concordance were at the same levels as when genotype calls were acquired using the previous protocol. Multiplex PCR with 192 pairs of locus-specific primers was available for target preparation in the DigiTag2 assay without the optimization of reaction conditions, and quality parameters had the same levels as those acquired with 96-plex PCR. The locus-specific primers were able to achieve sufficient (concentration of target amplicon ≥5 nM and specific (concentration of unexpected amplicons <2 nM amplification within 2 hours, were also able to achieve detectable amplifications even when working in a 96-plex or 192-plex form. The improved DigiTag2 assay will be an efficient platform for screening an intermediate number of SNPs (tens to hundreds of sites in the replication analysis after genome-wide association study. Moreover, highly parallel and short-acting amplification with locus-specific primers may thus facilitate widespread application to other PCR-based assays.

  18. Billfish Tagging

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The SWFSC's constituent-based Billfish Tagging Program began in 1963 and since that time has provided conventional spaghetti type tags and tagging supplies to...

  19. Ultrasensitive aptamer-based multiplexed electrochemical detection by coupling distinguishable signal tags with catalytic recycling of DNase I.

    Science.gov (United States)

    Tang, Dianping; Tang, Juan; Li, Qunfang; Su, Biling; Chen, Guonan

    2011-10-01

    This work reports an aptamer-based, disposable, and multiplexed sensing platform for simultaneous electrochemical determination of small molecules, employing adenosine triphosphate (ATP) and cocaine as the model target analytes. The multiplexed sensing strategy is based on target-induced release of distinguishable redox tag-conjugated aptamers from a magnetic graphene platform. The electronic signal of the aptasensors could be further amplified by coupling DNase I with catalytic recycling of self-produced reactants. The assay was based on the change in the current at the various peak potentials in the presence of the corresponding signal tags. Experimental results revealed that the multiplexed electrochemical aptasensor enabled the simultaneous monitoring of ATP and cocaine in a single run with wide working ranges and low detection limits (LODs: 0.1 pM for ATP and 1.5 pM for cocaine). This concept offers promise for rapid, simple, and cost-effective analysis of biological samples.

  20. Effective detection of human leukocyte antigen risk alleles in celiac disease using tag single nucleotide polymorphisms.

    Directory of Open Access Journals (Sweden)

    Alienke J Monsuur

    Full Text Available BACKGROUND: The HLA genes, located in the MHC region on chromosome 6p21.3, play an important role in many autoimmune disorders, such as celiac disease (CD, type 1 diabetes (T1D, rheumatoid arthritis, multiple sclerosis, psoriasis and others. Known HLA variants that confer risk to CD, for example, include DQA1*05/DQB1*02 (DQ2.5 and DQA1*03/DQB1*0302 (DQ8. To diagnose the majority of CD patients and to study disease susceptibility and progression, typing these strongly associated HLA risk factors is of utmost importance. However, current genotyping methods for HLA risk factors involve many reactions, and are complicated and expensive. We sought a simple experimental approach using tagging SNPs that predict the CD-associated HLA risk factors. METHODOLOGY: Our tagging approach exploits linkage disequilibrium between single nucleotide polymorphism (SNPs and the CD-associated HLA risk factors DQ2.5 and DQ8 that indicate direct risk, and DQA1*0201/DQB1*0202 (DQ2.2 and DQA1*0505/DQB1*0301 (DQ7 that attribute to the risk of DQ2.5 to CD. To evaluate the predictive power of this approach, we performed an empirical comparison of the predicted DQ types, based on these six tag SNPs, with those executed with current validated laboratory typing methods of the HLA-DQA1 and -DQB1 genes in three large cohorts. The results were validated in three European celiac populations. CONCLUSION: Using this method, only six SNPs were needed to predict the risk types carried by >95% of CD patients. We determined that for this tagging approach the sensitivity was >0.991, specificity >0.996 and the predictive value >0.948. Our results show that this tag SNP method is very accurate and provides an excellent basis for population screening for CD. This method is broadly applicable in European populations.

  1. Towards the use of protein A-tagged gold nanoparticles for signal amplification of electrochemical immunosensors in virus detection

    International Nuclear Information System (INIS)

    Huy Tran, Quang; Thuy Nguyen, Thanh; Chung Pham, Van; Hong Hanh Nguyen, Thi; Tuan Mai, Anh

    2012-01-01

    In this paper we represent a study on the potential use of protein A-tagged gold nanoparticles applied for signal amplification of electrochemical immunosensors. Gold nanoparticles (GNPs) were synthesized by the chemical reduction of tetrachloroauric (III) acid trihydrate using sodium ascorbate, and then tagged with protein A (PrA) via ultracentrifugation. UV-Vis spectroscopy and transmission electron microscopy were used to verify the characteristics of formed GNPs/PrA complex. The analyzed results indicate that GNPs were found spherically, homogeneously, and with an average diameter of about 10 nm. Immunoelectron microscopy was then used to investigate the bioactivity of the GNPs/PrA complex in solution by the effective binding of GNPs to viral particles. Scanning electron and fluorescence microscopies were also used to investigate the distribution and the bioactivity of the GNPs/PrA complex on the surface of the interdigitated sensor. Consequently, this study provided some assumptions of the potential application of protein A-tagged gold nanoparticles for signal amplification of electrochemical immunosensors in virus detection from clinical samples

  2. Detection of viable myocardium by dobutamine stress tagging magnetic resonance imaging with three-dimensional analysis by automatic trace method

    Energy Technology Data Exchange (ETDEWEB)

    Saito, Isao [Yatsu Hoken Hospital, Narashino, Chiba (Japan); Watanabe, Shigeru; Masuda, Yoshiaki

    2000-07-01

    The present study attempted to detect the viability of myocardium by quantitative automatic 3-dimensional analysis of the improvement of regional wall motion using an magnetic resonance imaging (MRI) tagging method. Twenty-two subjects with ischemic heart disease who had abnormal wall motion on echocardiography at rest were enrolled. All patients underwent dobutamine stress echocardiography (DSE), coronary arteriography and left ventriculography. The results were compared with those of 7 normal volunteers. MRI studies were done with myocardial tagging using the spatial modulation of magnetization technique. Automatic tracing with an original program was performed, and wall motion was compared before and during dobutamine infusion. The evaluation of myocardial viability with MRI and echocardiography had similar results in 19 (86.4%) of the 22 patients; 20 were studied by positron emission tomography or thallium-201 single photon emission computed tomography for myocardial viability, or studied for improvement of wall motion following coronary intervention. The sensitivity of dobutamine stress MRI (DSMRI) with tagging was 75.9% whereas that of DSE was 65.5%. The specificity of DSMRI was 85.7% (6/7) and that of DSE was 100% (7/7). The accuracy of DSMRI was 77.8% (28/36) and that of DSE 72.2% (26/36). DSMRI was shown to be superior to DSE in terms of evaluation of myocardial viability. (author)

  3. GFP-tagged multimetal-tolerant bacteria and their detection in the rhizosphere of white mustard

    OpenAIRE

    Piotrowska-Seget, Zofia; Beściak, Grażyna; Bernaś, Tytus; Kozdrój, Jacek

    2011-01-01

    The introduction of rhizobacteria that tolerate heavy metals is a promising approach to support plants involved in phytoextraction and phytostabilisation. In this study, soil of a metal-mine wasteland was analyzed for the presence of metal-tolerant bacterial isolates, and the tolerance patterns of the isolated strains for a number of heavy metals and antibiotics were compared. Several of the multimetal-tolerant strains were tagged with a broad host range reporter plasmid (i.e. pPROBE-NT) bear...

  4. Detection and correction of blinking bias in image correlation transport measurements of quantum dot tagged macromolecules

    DEFF Research Database (Denmark)

    Durisic, Nela; Bachir, Alexia I; Kolin, David L

    2007-01-01

    Semiconductor nanocrystals or quantum dots (QDs) are becoming widely used as fluorescent labels for biological applications. Here we demonstrate that fluorescence fluctuation analysis of their diffusional mobility using temporal image correlation spectroscopy is highly susceptible to systematic...... application of the image correlation methods for measurement of the diffusion coefficient of glycosyl phosphatidylinositol-anchored proteins tagged with QDs as imaged on living fibroblasts Udgivelsesdato: 2007-Aug-15...

  5. Raft and floating radio frequency identification (RFID) antenna systems for detecting and estimating abundance of PIT-tagged fish in rivers

    Science.gov (United States)

    Fetherman, Eric R.; Avila, Brian W.; Winkelman, Dana L.

    2016-01-01

    Portable radio frequency identification (RFID) PIT tag antenna systems are increasingly being used in studies examining aquatic animal movement, survival, and habitat use, and their design flexibility permits application in a wide variety of settings. We describe the construction, use, and performance of two portable floating RFID PIT tag antenna systems designed to detect fish that were unavailable for recapture using stationary antennas or electrofishing. A raft antenna system was designed to detect and locate PIT-tagged fish in relatively long (i.e., ≥10 km) river reaches, and consisted of two antennas: (1) a horizontal antenna (4 × 1.2 m) installed on the bottom of the raft and used to detect fish in shallower river reaches (test using rocks marked with 32-mm PIT tags. Detection probability of PIT-tagged fish in the Cache la Poudre River, Colorado, using the raft antenna system, which covered 21% of the wetted area, was 0.14 ± 0.14. A shore-deployed floating antenna (14.6 × 0.6 m), which covered 100% of the wetted area, was designed for use by two operators for detecting and locating PIT-tagged fish in shorter (i.e., alternative to estimating abundance using traditional sampling methods such as electrofishing.

  6. Ultrasound tagged near infrared spectroscopy does not detect hyperventilation-induced reduction in cerebral blood flow

    DEFF Research Database (Denmark)

    Lund, Anton; Secher, Niels H.; Hirasawa, Ai

    2016-01-01

    Introduction: Continuous non-invasive monitoring of cerebral blood flow (CBF) may be important during anaesthesia and several options are available. We evaluated the CerOx monitor that employs ultrasound tagged near infrared spectroscopy to estimate changes in a CBF index (CFI).Methods: Seven...... by transcranial Doppler. Blood flow in the internal and external carotid artery (ICAf and ECAf) was determined using duplex ultrasonography and forehead skin blood flow (SkBF) and oxygenation (SskinO2) by laser Doppler and white light spectroscopy.Results: During hyperventilation MCAvmean and ICAf decreased by 44...

  7. Measurement of $b$-tagging Efficiency of $c$-jets in $t\\bar{t}$ Events Using a Likelihood Approach with the ATLAS Detector

    CERN Document Server

    The ATLAS collaboration

    2018-01-01

    A new technique is presented to measure the rate at which charm jets are tagged as $b$-jets based on a data sample of single lepton $t\\bar{t}$ events, where one of the $W$-bosons decays leptonically and the other decays to a $c$- and $s$-quark, or other quark pair combinations. The data sample was collected by the ATLAS detector at $\\sqrt{s} = 13$ TeV in 2015 and 2016 and corresponds to an integrated luminosity of 36 fb$^{-1}$. A kinematic likelihood technique is used to assign jets to the corresponding $t\\bar{t}$ decay products. A likelihood fit is used to extract the $c$-jet tagging efficiency from the pair of jets associated to $W$-boson decays. This new technique is used to calibrate the ATLAS MV2c10 $b$-tagging algorithm.

  8. Protein fusion tags for efficient expression and purification of recombinant proteins in the periplasmic space of E. coli.

    Science.gov (United States)

    Malik, Ajamaluddin

    2016-06-01

    Disulfide bonds occurred in majority of secreted protein. Formation of correct disulfide bonds are must for achieving native conformation, solubility and activity. Production of recombinant proteins containing disulfide bond for therapeutic, diagnostic and various other purposes is a challenging task of research. Production of such proteins in the reducing cytosolic compartment of E. coli usually ends up in inclusion bodies formation. Refolding of inclusion bodies can be difficult, time and labor consuming and uneconomical. Translocation of these proteins into the oxidative periplasmic compartment provides correct environment to undergo proper disulfide bonds formation and thus achieving native conformation. However, not all proteins can be efficiently translocated to the periplasm with the help of bacterial signal peptides. Therefore, fusion to a small well-folded and stable periplasmic protein is more promising for periplasmic production of disulfide bonded proteins. In the past decades, several full-length proteins or domains were used for enhancing translocation and solubility. Here, protein fusion tags that significantly increase the yields of target proteins in the periplasmic space are reviewed.

  9. Acoustic tag detections of green sturgeon in the Columbia River and Coos Bay estuaries, Washington and Oregon, 2010–11

    Science.gov (United States)

    Hansel, Hal C.; Romine, Jason G.; Perry, Russell W.

    2017-11-08

    The Columbia River, in Washington and Oregon, and Coos Bay, in Oregon, are economically important shipping channels that are inhabited by several fishes protected under the Endangered Species Act (ESA). Maintenance of shipping channels involves dredge operations to maintain sufficient in-channel depths to allow large ships to navigate the waterways safely. Fishes entrained by dredge equipment often die or experience delayed mortality. Other potential negative effects of dredging include increased turbidity, reductions in prey resources, and the release of harmful contaminants from the dredged sediments. One species of concern is the ESA-listed green sturgeon (Acipenser medirostris; Southern Distinct Population Segment). In this study, we used acoustic telemetry to identify habitat use, arrival and departure timing, and the extent of upstream migration of green sturgeon in the Columbia River and Coos Bay to help inform dredge operations to minimize potential take of green sturgeon. Autonomous acoustic receivers were deployed in Coos Bay from the mouth to river kilometer (rkm) 21.6 from October 2009 through October 2010. In the Columbia River Estuary, receivers were deployed between the mouth and rkm 37.8 from April to November in 2010 and 2011. A total of 29 subadult and adult green sturgeon were tagged with temperature and pressure sensor tags and released during the study, primarily in Willapa Bay and Grays Harbor, Washington, and the Klamath River, Oregon. Green sturgeon detected during the study but released by other researchers also were included in the study.The number of tagged green sturgeon detected in the two estuaries differed markedly. In Coos Bay, only one green sturgeon was detected for about 2 hours near the estuary mouth. In the Columbia River Estuary, 9 green sturgeon were detected in 2010 and 10 fish were detected in 2011. Green sturgeon entered the Columbia River from May through October during both years, with the greatest numbers of fish being

  10. Fuzzy logic-based approach to detecting a passive RFID tag in an outpatient clinic.

    Science.gov (United States)

    Min, Daiki; Yih, Yuehwern

    2011-06-01

    This study is motivated by the observations on the data collected by radio frequency identification (RFID) readers in a pilot study, which was used to investigate the feasibility of implementing an RFID-based monitoring system in an outpatient eye clinic. The raw RFID data collected from RFID readers contain noise and missing reads, which prevent us from determining the tag location. In this paper, fuzzy logic-based algorithms are proposed to interpret the raw RFID data to extract accurate information. The proposed algorithms determine the location of an RFID tag by evaluating its possibility of presence and absence. To evaluate the performance of the proposed algorithms, numerical experiments are conducted using the data observed in the outpatient eye clinic. Experiments results showed that the proposed algorithms outperform existing static smoothing method in terms of minimizing both false positives and false negatives. Furthermore, the proposed algorithms are applied to a set of simulated data to show the robustness of the proposed algorithms at various levels of RFID reader reliability.

  11. Dark-lumen MR colonography with fecal tagging: a comparison of water enema and air methods of colonic distension for detecting colonic neoplasms

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez Gomez, Sonia; Pages Llinas, Mario; Juan Garcia, Carmen de; Rimola Gibert, Jordi; Ayuso Colella, Juan R.; Ayuso Colella, Carmen [Hospital Clinico of Barcelona, Department of Radiology, Barcelona (Spain); Castells Garangou, Antoni; Bordas Alsina, Josep M. [Hospital Clinico of Barcelona, Department of Gastroenterology, Barcelona (Spain)

    2008-07-15

    The purpose was to evaluate MR colonography (MRC) with barium fecal tagging in detecting colorectal pathology and to determine how air-based and water-based colonic distension influences MRC. We studied 83 patients with high risk of colonic neoplasms. All received oral barium sulfate for colonic preparation before unenhanced and enhanced T1-weighted gradient-echo MRC using either water (n=54) or air (n=29) for colonic distension. Fecal tagging, distension, and artifacts were recorded. All patients underwent conventional colonoscopy within 2 weeks of MRC; the techniques were compared for detection of malignant neoplasms and polyps {>=}1 cm, 6-9 mm, and {<=}5 mm. Fecal tagging was ''good'' in 76% of the colonic segments in water-distended patients and 46% of air-distended patients. The degree of distension was ''good'' in 90.7% of water-distended patients and 44% of air-distended patients. Severe artifacts were present in 15% air-distended patients and 0.3% of water-distended patients. Both water-distended and air-distended MRC detected all malignant neoplasms and polyps {>=}1 cm, but more air-distended MRC were excluded for poor quality. MRC with fecal tagging is useful for detecting lesions {>=}1 cm. Air distension was inferior to water distension in most aspects. Water-based colonic distension should be used for barium-tagging MRC. (orig.)

  12. Dark-lumen MR colonography with fecal tagging: a comparison of water enema and air methods of colonic distension for detecting colonic neoplasms

    International Nuclear Information System (INIS)

    Rodriguez Gomez, Sonia; Pages Llinas, Mario; Juan Garcia, Carmen de; Rimola Gibert, Jordi; Ayuso Colella, Juan R.; Ayuso Colella, Carmen; Castells Garangou, Antoni; Bordas Alsina, Josep M.

    2008-01-01

    The purpose was to evaluate MR colonography (MRC) with barium fecal tagging in detecting colorectal pathology and to determine how air-based and water-based colonic distension influences MRC. We studied 83 patients with high risk of colonic neoplasms. All received oral barium sulfate for colonic preparation before unenhanced and enhanced T1-weighted gradient-echo MRC using either water (n=54) or air (n=29) for colonic distension. Fecal tagging, distension, and artifacts were recorded. All patients underwent conventional colonoscopy within 2 weeks of MRC; the techniques were compared for detection of malignant neoplasms and polyps ≥1 cm, 6-9 mm, and ≤5 mm. Fecal tagging was ''good'' in 76% of the colonic segments in water-distended patients and 46% of air-distended patients. The degree of distension was ''good'' in 90.7% of water-distended patients and 44% of air-distended patients. Severe artifacts were present in 15% air-distended patients and 0.3% of water-distended patients. Both water-distended and air-distended MRC detected all malignant neoplasms and polyps ≥1 cm, but more air-distended MRC were excluded for poor quality. MRC with fecal tagging is useful for detecting lesions ≥1 cm. Air distension was inferior to water distension in most aspects. Water-based colonic distension should be used for barium-tagging MRC. (orig.)

  13. Demonstration of the BNL Continuous Dual Trap Analyzer to Detect Perfluorocarbon Tracers for the Tag, Track and Location Program

    Energy Technology Data Exchange (ETDEWEB)

    Heiser,J.H.; Adams, J.; Dietz, R..; Milian, L.; Watson, T.

    2008-10-07

    The Tag, Track and Location System (TTL) Program is investigating methods of tracking an asset using perfluorocarbon tracers (PFT). The success of any TTL method requires sound detection/location instrumentation. Tracer Detection Technologies Corp (TDT), through a contract with the Office of Naval Research (ONR), is investigating different detection systems. The detections systems generally fall into two categories; proximity detectors and standoff detectors. Proximity detectors, as the name implies, need to be in close proximity (e.g., meter to 10's of meters) to the PFT source. Standoff detection searches for the PFT from a greater distance away from the source (e.g., 100's of meters to kilometers). Gas Chromatographs (GC) are generally considered a proximity detection systems, but in the case of PFTs should be considered for both proximity and standoff detection with the caveat that in standoff use the GC needs to be somewhere in the PFT plume, i.e., generally downwind of the source. With a properly sized PFT source, the right GC can afford fairly large standoff (distance from the source) distances; 100's of meters to kilometers downwind. Brookhaven National Laboratory (BNL) has such a GC system and offered to demonstrate the CDTA for TTL as a no cost addition to the TDTTTL project, of which BNL was a participant. BNL is a leading authority on the sampling, collection, release and detection of PFTs. In addition, the BNL team has extensive background in atmospheric dispersion, the application of PFTs to such studies and the development of applications utilizing PFTs such as building infiltration measurements, control room integrity determination, leak location and environmental investigations. This experience and expertise is essential in developing any PFT application were dispersion, dilution and overcoming environmental conditions and interferences are integral to success. BNL has developed sophisticated gas chromatography methods and

  14. A study of B0-B0(bar) oscillations frequency and determination of flavor-tagging efficiency using semileptonic and hadronic B0 decays

    Energy Technology Data Exchange (ETDEWEB)

    Barrera, Barbara

    2000-10-13

    B{sup 0}{bar B}{sup 0} flavor oscillations are studied in e{sup +}e{sup -} annihilation data collected with the BABAR detector at center-of-mass energies near the {Upsilon}(4S) resonance. One B is reconstructed in a hadronic or semileptonic decay mode, and the flavor of the other B in the event is determined with a tagging algorithm that exploits the relation between the flavor of the heavy quark and the charges of its decay products. Tagging performance is characterized by an efficiency {epsilon}{sub i} and a probability for mis-identification, w{sub i}, for each tagging category. We report a determination of the wrong-tag probabilities, w{sub i}, and a preliminary result for the time-dependent B{sup 0}{bar B}{sup 0} oscillation frequency, {Delta}m{sub d} = 0.512 {+-} 0.017 {+-} 0.022 {Dirac_h} ps{sup -1}.

  15. Computer assisted detection software for CT colonography: effect of sphericity filter on performance characteristics for patients with and without fecal tagging

    International Nuclear Information System (INIS)

    Dehmeshki, Jamshid; McQuillan, Justine; Honeyfield, Lesley; Amin, Hamdan; Halligan, Steve; Taylor, Stuart A.; Roddie, Mary E.

    2007-01-01

    The aim of this study is to investigate the effect of changing sphericity filter values on performance of a computer assisted detection (CAD) system for CT colonography for data with and without fecal tagging. Colonography data from 138 patients with 317 validated polyps were divided into those with (86) and without (52) fecal tagging. Polyp coordinates were established by three observers and datasets analysed subsequently by a proprietary CAD system used at four discrete sphericity filter settings. Prompts were compared with the known coordinates in order to determine sensitivity and specificity. Sensitivity was highest at low sphericity; of 164 polyps 6 mm or more, 144 (87.8%) were detected at sphericity 0.3, and 132 (80.1%) at sphericity 0.9. Of 42 polyps measuring 10 mm or more, 40 (95.2%) were detected at sphericity 0.3, and 36 (85.7%) at sphericity 0.9. There was no significant difference in sensitivity for tagged and un-tagged data but specificity was reduced in tagged data at low sphericity and significantly reduced in untagged data at high sphericity. CAD had a sensitivity of 95.2% for polyps measuring 1 cm or more and 87.8% for polyps 6 mm or more when used at a sphericity setting of 0.3. Higher sphericity settings increased specificity while reducing sensitivity. The bowel preparation used significantly impacts on specificity. (orig.)

  16. Raft and floating radio frequency identification (RFID) antenna systems for detecting and estimating abundance of PIT-tagged fish in rivers

    Science.gov (United States)

    Fetherman, Eric R.; Avila, Brian W.; Winkelman, Dana L.

    2016-01-01

    Portable radio frequency identification (RFID) PIT tag antenna systems are increasingly being used in studies examining aquatic animal movement, survival, and habitat use, and their design flexibility permits application in a wide variety of settings. We describe the construction, use, and performance of two portable floating RFID PIT tag antenna systems designed to detect fish that were unavailable for recapture using stationary antennas or electrofishing. A raft antenna system was designed to detect and locate PIT-tagged fish in relatively long (i.e., ≥10 km) river reaches, and consisted of two antennas: (1) a horizontal antenna (4 × 1.2 m) installed on the bottom of the raft and used to detect fish in shallower river reaches (<1 m), and (2) a vertical antenna (2.7 × 1.2 m) for detecting fish in deeper pools (≥1 m). Detection distances of the horizontal antenna were between 0.7 and 1.0 m, and detection probability was 0.32 ± 0.02 (mean ± SE) in a field test using rocks marked with 32-mm PIT tags. Detection probability of PIT-tagged fish in the Cache la Poudre River, Colorado, using the raft antenna system, which covered 21% of the wetted area, was 0.14 ± 0.14. A shore-deployed floating antenna (14.6 × 0.6 m), which covered 100% of the wetted area, was designed for use by two operators for detecting and locating PIT-tagged fish in shorter (i.e., <2 km) river reaches. Detection distances of the shore-deployed floating antenna were between 0.7 and 0.8 m, and detection probabilities during field deployment in the St. Vrain River exceeded 0.52. The shore-deployed floating antenna was also used to estimate abundance of PIT-tagged fish. Results suggest that the shore-deployed floating antenna could be used as an alternative to estimating abundance using traditional sampling methods such as electrofishing.

  17. TagCleaner: Identification and removal of tag sequences from genomic and metagenomic datasets

    Directory of Open Access Journals (Sweden)

    Lim Yan

    2010-06-01

    Full Text Available Abstract Background Sequencing metagenomes that were pre-amplified with primer-based methods requires the removal of the additional tag sequences from the datasets. The sequenced reads can contain deletions or insertions due to sequencing limitations, and the primer sequence may contain ambiguous bases. Furthermore, the tag sequence may be unavailable or incorrectly reported. Because of the potential for downstream inaccuracies introduced by unwanted sequence contaminations, it is important to use reliable tools for pre-processing sequence data. Results TagCleaner is a web application developed to automatically identify and remove known or unknown tag sequences allowing insertions and deletions in the dataset. TagCleaner is designed to filter the trimmed reads for duplicates, short reads, and reads with high rates of ambiguous sequences. An additional screening for and splitting of fragment-to-fragment concatenations that gave rise to artificial concatenated sequences can increase the quality of the dataset. Users may modify the different filter parameters according to their own preferences. Conclusions TagCleaner is a publicly available web application that is able to automatically detect and efficiently remove tag sequences from metagenomic datasets. It is easily configurable and provides a user-friendly interface. The interactive web interface facilitates export functionality for subsequent data processing, and is available at http://edwards.sdsu.edu/tagcleaner.

  18. Surface-Enhanced Raman Spectroscopy forStaphylococcus aureusDNA Detection by Using Surface-Enhanced Raman Scattering Tag on Au Film Over Nanosphere Substrate.

    Science.gov (United States)

    Chen, Jian; Wang, Jun-Feng; Wu, Xue-Zhong; Rong, Zhen; Dong, Pei-Tao; Xiao, Rui

    2018-06-01

    We developed a high-performance surface-enhanced Raman scattering (SERS) sensing platform that can be used for specific and sensitive DNA detection. The SERS platform combines the advantages of Au film over nanosphere (AuFON) substrate and Ag@PATP@SiO2 SERS tag. SERS tag-on-AuFON is a sensing system that operates by the self-assembly of SERS tag onto an AuFON substrate in the presence of target DNAs. The SERS signals can be dramatically enhanced by the formation of "hot spots" in the interstices between the assembled nanostructures, as confirmed by finite-difference time-domain (FDTD) simulation. As a new sensing platform, SERS tag-on-AuFON was utilized to detect Staphylococcus aureus (S. aureus) DNA with a limit of detection at 1 nM. A linear relationship was also observed between the SERS intensity at Raman peak 1439 cm-1 and the logarithm of target DNA concentrations ranging from 1 μM to 1 nM. Besides, the sensing platform showed good homogeneity, with a relative standard deviation of about 1%. The sensitive SERS platform created in this study is a promising tool for detecting trace biochemical molecules because of its relatively simple and effective fabrication procedure, high sensitivity, and high reproducibility of the SERS effect.

  19. Using Lanthanide Nanoparticles as Isotopic Tags for Biomarker Detection by Mass Cytometry

    Science.gov (United States)

    Cao, Pengpeng

    The development of robust, versatile, and high-throughput biosensing techniques has widespread implications for early disease detection and accurate diagnosis. An innovative technology, mass cytometry, has been developed to use isotopically-labelled antibodies to simultaneously study multiple parameters of single cells. The current detection sensitivity of mass cytometry is limited by the number of copies of a given isotope that can be attached to a given antibody. This thesis describes research on the synthesis, characterization, and bioconjugation of a new class of nanoparticle-based labelling agents to be employed for the detection of low-abundance biomarkers by mass cytometry. Hydrophobic lanthanide nanoparticles (Ln NPs) have been prepared by the Winnik group. To render the NPs water-soluble for biological applications, we coated the NP surface with a first generation of multidentate poly(ethylene glycol) (PEG)-based ligands via ligand exchange. We measured the size, morphology, and polydispersity of these hydrophilic NPs by transmission electron microscopy (TEM) and dynamic light scattering (DLS). The colloidal stability of the NPs was determined at various pH and in phosphate buffered saline (PBS) solutions. Tetradentate-PEG-coated NPs (Tetra-NPs) exhibited the best stability at pH 3 to 9, and in PBS. However, when cells were treated with Tetra-NPs in preliminary in vitro studies, significant undesirable non-specific binding (NSB) was observed. In order to tackle the NSB issue presented in the Tetra-NPs, we prepared a second generation of polymer-based ligands using ring-opening metathesis polymerization (ROMP). A small library of ROMP polymers was synthesized, characterized, and used to stabilize NPs in aqueous solutions. The ROMP-NPs were found to have significantly reduced NSB to cells by inductively coupled plasma-mass spectrometry (ICP-MS). To further modify the NPs, amine groups were introduced as functional handles to both the tetradentate-PEG and

  20. A Fluorogenic Probe for the Catalyst-Free Detection of Azide-Tagged Molecules

    Science.gov (United States)

    Friscourt, Frédéric; Fahrni, Christoph J.; Boons, Geert-Jan

    2012-01-01

    Fluorogenic reactions in which non- or weakly-fluorescent reagents produce highly fluorescent products can be exploited to detect a broad range of compounds including biomolecules and materials. We describe a modified dibenzocyclooctyne that under catalyst-free conditions undergoes fast strain-promoted cycloadditions with azides to yield strongly fluorescent triazoles. The cycloaddition products are more than 1000-fold brighter compared to the starting cyclooctyne, exhibit large Stokes shift, and can be excited above 350 nm, which is required for many applications. Quantum mechanical calculations indicate that the fluorescence increase upon triazole formation is due to large differences in oscillator strengths of the S0 S1 transitions in the planar C2v-symmetric starting material compared to the symmetry-broken and non-planar cycloaddition products. The new fluorogenic probe was successfully employed for labeling of proteins modified by an azide moiety. PMID:23095037

  1. First Results in the Use of Bovine Ear Notch Tag for Bovine Viral Diarrhoea Virus Detection and Genetic Analysis.

    Directory of Open Access Journals (Sweden)

    Christian Quinet

    Full Text Available Infection due to bovine viral diarrhoea virus (BVDV is endemic in most cattle-producing countries throughout the world. The key elements of a BVDV control programme are biosecurity, elimination of persistently infected animals and surveillance. Bovine viral diarrhoea (BVD is a notifiable disease in Belgium and an official eradication programme started from January 2015, based on testing ear notches sampled during the official identification and registration of calves at birth. An antigen-capture ELISA test based on the detection of BVDV Erns protein is used. Ear notch sample may also be used to characterize the genotype of the calf when appropriate elution/dilution buffer is added. Both BVDV antigen-ELISA analysis and animal traceability could be performed.With regards to the reference protocol used in the preparation of ear notch samples, alternative procedures were tested in terms of BVDV analytic sensitivity, diagnostic sensitivity and specificity, as well as quality and purity of animal DNA.The Allflex DNA Buffer D showed promising results in BVDV diagnosis and genome analyses, opening new perspectives for the livestock industry by the exploitation of the animal genome. Due to the high number of cattle involved in the Belgian official BVDV eradication programme based on ear notch tags sample, a large database on both BVDV status of newborn calves and cattle genome could be created for subsequent different uses (e.g. traceability, determination of parentage, genetic signatures throughout the genome associated with particular traits evolving through a more integrated animal health.

  2. Effective and efficient model clone detection

    DEFF Research Database (Denmark)

    Störrle, Harald

    2015-01-01

    Code clones are a major source of software defects. Thus, it is likely that model clones (i.e., duplicate fragments of models) have a significant negative impact on model quality, and thus, on any software created based on those models, irrespective of whether the software is generated fully...... automatically (“MDD-style”) or hand-crafted following the blueprint defined by the model (“MBSD-style”). Unfortunately, however, model clones are much less well studied than code clones. In this paper, we present a clone detection algorithm for UML domain models. Our approach covers a much greater variety...... of model types than existing approaches while providing high clone detection rates at high speed....

  3. Efficient online detection of temporal patterns

    Directory of Open Access Journals (Sweden)

    Shlomi Dolev

    2016-04-01

    Full Text Available Identifying a temporal pattern of events is a fundamental task of online (real-time verification. We present efficient schemes for online monitoring of events for identifying desired/undesired patterns of events. The schemes use preprocessing to ensure that the number of comparisons during run-time is minimized. In particular, the first comparison following the time point when an execution sub-sequence cannot be further extended to satisfy the temporal requirements halts the process that monitors the sub-sequence.

  4. Addition of urea and thiourea to electrophoresis sample buffer improves efficiency of protein extraction from TCA/acetone-treated smooth muscle tissues for phos-tag SDS-PAGE.

    Science.gov (United States)

    Takeya, Kosuke; Kaneko, Toshiyuki; Miyazu, Motoi; Takai, Akira

    2018-01-01

    Phosphorylation analysis by using phos-tag technique has been reported to be suitable for highly sensitive quantification of smooth muscle myosin regulatory light chain (LC 20 ) phosphorylation. However, there is another factor that will affect the sensitivity of phosphorylation analysis, that is, protein extraction. Here, we optimized the conditions for total protein extraction out of trichloroacetic acid (TCA)-fixed tissues. Standard SDS sample buffer extracted less LC 20 , actin and myosin phosphatase targeting subunit 1 (MYPT1) from TCA/acetone treated ciliary muscle strips. On the other hand, sample buffer containing urea and thiourea in addition to lithium dodecyl sulfate (LDS) or SDS extracted those proteins more efficiently, and thus increased the detection sensitivity up to 4-5 fold. Phos-tag SDS-PAGE separated dephosphorylated and phosphorylated LC 20 s extracted in LDS/urea/thiourea sample buffer to the same extent as those in standard SDS buffer. We have concluded that LDS (or SDS) /urea/thiourea sample buffer is suitable for highly sensitive phosphorylation analysis in smooth muscle, especially when it is treated with TCA/acetone. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. Evaluation of Luminex xTAG Gastrointestinal Pathogen Panel Assay for Detection of Multiple Diarrheal Pathogens in Fecal Samples in Vietnam.

    Science.gov (United States)

    Duong, Vu Thuy; Phat, Voong Vinh; Tuyen, Ha Thanh; Dung, Tran Thi Ngoc; Trung, Pham Duc; Minh, Pham Van; Tu, Le Thi Phuong; Campbell, James I; Le Phuc, Hoang; Ha, Ton Thi Thanh; Ngoc, Nguyen Minh; Huong, Nguyen Thi Thanh; Tam, Pham Thi Thanh; Huong, Dang Thao; Xang, Nguyen Van; Dong, Nguyen; Phuong, Le Thi; Hung, Nguyen Van; Phu, Bui Duc; Phuc, Tran My; Thwaites, Guy E; Vi, Lu Lan; Rabaa, Maia A; Thompson, Corinne N; Baker, Stephen

    2016-04-01

    Diarrheal disease is a complex syndrome that remains a leading cause of global childhood morbidity and mortality. The diagnosis of enteric pathogens in a timely and precise manner is important for making treatment decisions and informing public health policy, but accurate diagnosis is a major challenge in industrializing countries. Multiplex molecular diagnostic techniques may represent a significant improvement over classical approaches. We evaluated the Luminex xTAG gastrointestinal pathogen panel (GPP) assay for the detection of common enteric bacterial and viral pathogens in Vietnam. Microbiological culture and real-time PCR were used as gold standards. The tests were performed on 479 stool samples collected from people admitted to the hospital for diarrheal disease throughout Vietnam. Sensitivity and specificity were calculated for the xTAG GPP for the seven principal diarrheal etiologies. The sensitivity and specificity for the xTAG GPP were >88% for Shigellaspp.,Campylobacterspp., rotavirus, norovirus genotype 1/2 (GI/GII), and adenovirus compared to those of microbiological culture and/or real-time PCR. However, the specificity was low (∼60%) for Salmonella species. Additionally, a number of important pathogens that are not identified in routine hospital procedures in this setting, such as Cryptosporidiumspp. and Clostridium difficile, were detected with the GPP. The use of the Luminex xTAG GPP for the detection of enteric pathogens in settings, like Vietnam, would dramatically improve the diagnostic accuracy and capacity of hospital laboratories, allowing for timely and appropriate therapy decisions and a wider understanding of the epidemiology of pathogens associated with severe diarrheal disease in low-resource settings. Copyright © 2016 Duong et al.

  6. Developing detection efficiency standards for atom probe tomography

    Science.gov (United States)

    Prosa, Ty J.; Geiser, Brian P.; Lawrence, Dan; Olson, David; Larson, David J.

    2014-08-01

    Atom Probe Tomography (APT) is a near-atomic-scale analytical technique which, due to recent advances in instrumentation and sample preparation techniques, is being used on a variety of 3D applications. Total system detection efficiency is a key parameter for obtaining accurate spatial reconstruction of atomic coordinates from detected ions, but experimental determination of efficiency can be difficult. This work explores new ways to measure total system detection efficiency as well as the specimen characteristics necessary for such measurements. Composite specimens composed of a nickel/chromium multilayer core, National Institute of Standards and Technology Standard Reference Material 2135c, encapsulated with silver, silicon, or nickel were used to demonstrate the suitability of this approach for providing a direct measurement of APT efficiency. Efficiency measurements based on this multilayer encapsulated in nickel are reported.

  7. UTag: Long-range Ultra-wideband Passive Radio Frequency Tags

    Energy Technology Data Exchange (ETDEWEB)

    Dowla, F

    2007-03-14

    Long-range, ultra-wideband (UWB), passive radio frequency (RF) tags are key components in Radio Frequency IDentification (RFID) system that will revolutionize inventory control and tracking applications. Unlike conventional, battery-operated (active) RFID tags, LLNL's small UWB tags, called 'UTag', operate at long range (up to 20 meters) in harsh, cluttered environments. Because they are battery-less (that is, passive), they have practically infinite lifetimes without human intervention, and they are lower in cost to manufacture and maintain than active RFID tags. These robust, energy-efficient passive tags are remotely powered by UWB radio signals, which are much more difficult to detect, intercept, and jam than conventional narrowband frequencies. The features of long range, battery-less, and low cost give UTag significant advantage over other existing RFID tags.

  8. Zn(II)-Phos-Tag SDS-PAGE for Separation and Detection of a DNA Damage-Related Signaling Large Phosphoprotein.

    Science.gov (United States)

    Kinoshita, Eiji; Kinoshita-Kikuta, Emiko; Koike, Tohru

    2017-01-01

    In this chapter, we provide a standard protocol for phosphate-affinity sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Zn 2+ -Phos-tag SDS-PAGE). This technique uses a dizinc(II) complex of the phosphate-binding molecule Phos-tag in conjunction with a neutral-pH gel system, Tris [tris(hydroxymethyl)aminomethane], and acetic acid (Tris-AcOH), to detect shifts in the mobility of phosphorylated ataxia telangiectasia-mutated (ATM) kinase. This protocol, which employs a 3% (w/v) polyacrylamide gel strengthened with 0.5% (w/v) agarose, permits the separation of larger phosphoproteins with molecular masses in the order of 200 kDa over a period of approximately 4 h. Subsequently, multiple phosphorylated forms of high-molecular-mass ATM kinase (350 kDa) can be clearly detected via immunoblotting as multiple upshifted migration bands on the Zn 2+ -Phos-tag SDS-PAGE gel. The procedure described in this protocol requires a completion time of approximately 5 h from the beginning of gel preparation to the end of electrophoresis.

  9. Using Digital Acoustic Recording Tags to Detect Marine Mammals on Navy Ranges and Study their Responses to Naval Sonar

    Science.gov (United States)

    2011-02-01

    densirostris) off the coast of El Hierro in the Canary Islands. A tag was deployed with relative ease on an adult beaked whale which proceeded to carry it for...located off-shore of El Hierro by chance. On examination, we found that water ingress had again caused failure of the recording circuit early in the

  10. Sorption of his-tagged Protein G and Protein G onto chitosan/divalent metal ion sorbent used for detection of microcystin-LR.

    Science.gov (United States)

    Demey, Hary; Tria, Scherrine A; Soleri, Romain; Guiseppi-Elie, Anthony; Bazin, Ingrid

    2017-01-01

    A highly sensitive, specific, simple, and rapid chemiluminescence enzyme immunoassay (CLEIA) was developed for the determination of microcystin-LR (MC-LR) by using strategies for oriented immobilization of functionally intact polyclonal antibodies on chitosan surface. Several physicochemical parameters such as metal ion adsorption, hexahistidine-tagged Protein G sorption, the dilution ratio polyclonal antibody concentration, and peroxidase-labeled MC-LR concentration were studied and optimized. The sorption in batch system of G-histidine and G-proteins was studied on a novel sorbent consisting of chitosan/divalent metal ions. Transition metals as Ni ++ and Zn ++ were immobilized through interaction with -NH 2 groups of chitosan in order to supply a material capable to efficiently remove the proteins from aqueous solutions. The maximum uptake of divalent metals onto the chitosan material was found to be 230 mg g -1 for Zn ++ and 62 mg g -1 for Ni ++ . Experimental data were evaluated using the Langmuir and Freundlich models; the results were well fitted with the Langmuir model; chitosan/Ni ++ foam was found to be the best sorbent for G-protein, maximum sorption capacity obtained was 17 mg g -1 , and chitosan/Zn ++ was found to be the best for G-histidine with a maximum sorption capacity of 44 mg g -1 . Kinetic data was evaluated with pseudo-first- and pseudo-second-order models; the sorption kinetics were in all cases better represented by a pseudo-second-order model. Under optimum conditions, the calibration curve obtained for MC-LR gave detection limits of 0.5 ± 0.06 μg L -1 , the 50 % inhibition concentration (IC50) was 2.75 ± 0.03 μg L -1 , and the quantitative detection range was 0.5-25 μg L -1 . The limit of detection (LOD) attained from the calibration curves and the results obtained demonstrate the potential use of CLEIA with chitosan support as a screening tool for the analysis of pollutants in environmental samples.

  11. Monitoring and localization of buried plastic natural gas pipes using passive RF tags

    Science.gov (United States)

    Mondal, Saikat; Kumar, Deepak; Ghazali, Mohd. Ifwat; Chahal, Prem; Udpa, Lalita; Deng, Yiming

    2018-04-01

    A passive harmonic radio frequency (RF) tag on the pipe with added sensing capabilities is proposed in this paper. Radio frequency identification (RFID) based tagging has already emerged as a potential solution for chemical sensing, location detection, animal tagging, etc. Harmonic transponders are already quite popular compared to conventional RFIDs due to their improved signal to noise ratio (SNR). However, the operating frequency, transmitted power and tag efficiency become critical issues for underground RFIDs. In this paper, a comprehensive on-tag sensing, power budget and frequency analyses is performed for buried harmonic tag design. Accurate tracking of infrastructure burial depth is proposed to reduce the probability of failure of underground pipelines. Burial depth is estimated using phase information of received signals at different frequencies calculated using genetic algorithm (GA) based optimization for post processing. Suitable frequency range is determined for a variety of soil with different moisture content for small tag-antenna size. Different types of harmonic tags such as 1) Schottky diode, 2) Non-linear Transmission Line (NLTL) were compared for underground applications. In this study, the power, frequency and tag design have been optimized to achieve small antenna size, minimum signal loss and simple reader circuit for underground detection at up to 5 feet depth in different soil medium and moisture contents.

  12. Neutron detection efficiency for the measurement of the

    International Nuclear Information System (INIS)

    T. Eden; R. Madey; P. Markowitz; P.M. Rutt; B.D. Anderson; A.R. Baldwin; D. Barkhuff; K.B.Beard; W. Bertozzi; J.M. Cameron; C.C. Chang; G.W. Dodson; K. Dow; M. Farkhondeh; J.M. Finn; B.S. Flanders; C. Hyde-Wright; W.-D. Jiang; D. Keane; J.J. Kelly; W. Korsch; S. Kowalski; R. Lourie; J. McIntyre; D.M. Manley; Jean Mougey; B. Ni; T. Payerle; P.J. Pella; T. Reichelt; M. Spraker; D. Tieger; W. Turchinetz; Paul E. Ulmer; S. Van Verst; J. W. Watson; L.B. Weinstein; R. Roy Whitney; W.-M. Zhang

    1998-01-01

    We report a measurement of the neutron detection efficiency for an array of neutron detectors behind a lead-steel wall consisting of 10.16 cm thick lead bricks sandwiched between 3.18 cm steel plates. The neutron detection efficiency was obtained from the ratio of coincidence to single-arm proton cross sections using the gamma+ 2 1 H -->p+n reaction. Knowledge of the neutron detection efficiency was necessary to extract the cross section for the exclusive 2 H(e,e'n) 1 H reaction, which we used to obtain the elastic magnetic form factor G n M of the neutron. The measurements for three different neutron energies were carried out at the MIT-Bates Linear Accelerator Center with an incident electron beam energy of 254 MeV

  13. Study of an efficient application of the tagged bremsstrahlung in double-polarization experiments in the GeV range and the use of the inelastic electron scattering under extremely forward angles as alternative to the tagged bremsstrahlung

    International Nuclear Information System (INIS)

    Konrad, M.

    2006-03-01

    For the preparation of photonic probes for hadron physics the determination of energy and polarization of the photons is essential. In this dissertation in a first part a possibility of the determination of the degree of polarization by use of the asymmetry observables is presented. In a second part a possibility isd discussed to perform an energy and polarization tagging of nearly real photons in electron scattering under small Q 2 . By this method it should be possible to tag billions of photons per second

  14. An efficient circle detector not relying on edge detection

    Science.gov (United States)

    Cai, Jia; Huang, Panfeng; Chen, Lu; Zhang, Bin

    2016-06-01

    Accurate and efficient detection of circular modules fixed on non-cooperative target is a key technology for Tethered Space Robot. This paper presents an efficient circle detector based on region-growing of gradient and histogram distribution of Euclidean distance. Region-growing of gradient is applied to generate arc support regions from single point. And the corresponding square fitting areas are defined to accelerate the detection and decrease storage. A histogram is then used to count frequency of the distances that participates in the accumulator and the parameters of each circle are acquired. Finally, a verification strategy of circular integrity is designed to test the detection results. We have tested our algorithm on 35 images dealing with kinds of circles and ellipses. Experimental results demonstrate that our method is able to detect circular objects under occlusion, image noises and moderate shape deformations with a good precision.

  15. Detective quantum efficiency of medical x-ray image intensifiers

    CSIR Research Space (South Africa)

    Thirlwall, JT

    1998-11-01

    Full Text Available The recognition of small features of low contrast in the image recorded by an x-ray image intensifier is usually limited by photon noise. This detail-contrast perceptibility is dependent on the detective quantum efficiency (DQE) and the modulation...

  16. b-tagging in DELPHI at LEP

    CERN Document Server

    Abdallah, J; Adam, W; Adye, T; Adzic, P; Albrecht, T; Alderweireld, T; Alemany-Fernandez, R; Allmendinger, T; Allport, P P; Almehed, S; Amaldi, Ugo; Amapane, N; Amato, S; Anashkin, E; Andreazza, A; Andringa, S; Anjos, N; Antilogus, P; Apel, W D; Arnoud, Y; Ask, S; Åsman, B; Augustin, J E; Augustinus, A; Baillon, Paul; Ballestrero, A; Bambade, P; Barbier, R; Bardin, Dimitri Yuri; Barker, G; Baroncelli, A; Bates, M; Battaglia, Marco; Baubillier, M; Becks, K H; Begalli, M; Behrmann, A; Benekos, N C; Benvenuti, Alberto C; Bérat, C; Berggren, M; Berntzon, L; Bertrand, D; Besançon, M; Besson, N; Bibby, J; Biffi, P; Bloch, D; Blom, M; Bonesini, M; Boonekamp, M; Booth, P S L; Borisov, G; Botner, O; Bouquet, B; Bowcock, T J V; Boyko, I; Bracko, M; Branchini, P; Brenner, R; Brodet, E; Brückman, P; Brunet, J M; Bugge, L; Buschmann, P; Caccia, M; Calvi, M; Camporesi, T; Canale, V; Carena, F; Castro, N; Cavallo, F R; Chabaud, V; Chapkin, M M; Charpentier, P; Checchia, P; Chierici, R; Shlyapnikov, P; Chudoba, J; Chung, S U; Cieslik, K; Collins, P; Contri, R; Cosme, G; Cossutti, F; Costa, M J; Couchot, F; Crawley, B; Crennell, D J; Cuevas-Maestro, J; D'Almagne, B; D'Hondt, J; Dalmau, J; Da Silva, T; Da Silva, W; Della Ricca, G; De Angelis, A; de Boer, Wim; De Clercq, C; De Lotto, B; De Maria, N; De Min, A; De Paula, L S; Di Ciaccio, Lucia; Dijkstra, H; Di Simone, A; Doroba, K; Drees, J; Dris, M; Eigen, G; Ekelöf, T J C; Ellert, M; Elsing, M; Espirito-Santo, M C; Fanourakis, G K; Fassouliotis, D; Feindt, M; Fernández, J; Ferrer, A; Ferro, F; Flagmeyer, U; Föth, H; Fokitis, E; Fulda-Quenzer, F; Fuster, J A; Gandelman, M; García, C; Gavillet, P; Gazis, E N; Geralis, T; Gokieli, R; Golob, B; Gómez-Cadenas, J J; Gómez-Ceballos, G; Gonçalves, P; Graziani, E; Grosdidier, G; Grzelak, K; Guy, J; Haag, C; Hallgren, A; Hamacher, K; Hamilton, K; Hansen, J; Haug, S; Hauler, F; Hedberg, V; Hennecke, M; Hernando, J A; Herr, H; Heuser, J M; Holmgren, S O; Holt, P J; Houlden, M A; Hultqvist, K; Jackson, J N; Jalocha, P; Jarlskog, C; Jarlskog, G; Jarry, P; Jeans, D; Johansson, E K; Johansson, P D; Jonsson, P; Joram, C; Jungermann, L; Kapusta, F; Karlsson, M; Katsanevas, S; Katsoufis, E C; Keränen, R; Kernel, G; Kersevan, Borut P; Kiiskinen, A P; King, B T; Kjaer, N J; Kluit, P; Kokkinias, P; Kourkoumelis, C; Kuznetsov, O; Krumshtein, Z; Kucharczyk, M; Kucewicz, W; Kurowska, J; Lamsa, J; Leder, G; Ledroit, F; Leinonen, L; Leitner, R; Lemonne, J; Lepeltier, V; Lesiak, T; Liebig, W; Liko, D; Lipniacka, A; Lopes, J H; López, J M; Loukas, D; Lutz, P; Lyons, L; MacNaughton, J; Malek, A; Maltezos, S; Mandl, F; Marco, J; Marco, R; Maréchal, B; Margoni, M; Marin, J C; Mariotti, C; Markou, A; Martínez-Rivero, C; Martínez-Vidal, F; Masik, J; Mastroyiannopoulos, N; Matorras, F; Matteuzzi, C; Mazzucato, F; Mazzucato, M; McNulty, R; Meroni, C; Meyer, W T; Migliore, E; Mitaroff, W A; Mjörnmark, U; Moa, T; Moch, M; Mönig, K; Monge, R; Montenegro, J; Moraes, D; Moreno, S; Morettini, P; Müller, U; Münich, K; Mulders, M; Mundim, L; Murray, W; Muryn, B; Myatt, Gerald; Myklebust, T; Nassiakou, M; Navarria, Francesco Luigi; Nawrocki, K; Nicolaidou, R; Niezurawski, P; Nikolenko, M; Nomerotski, A; Norman, A; Nygren, A; Oblakowska-Mucha, A; Obraztsov, V F; Olshevskii, A G; Onofre, A; Orava, Risto; Österberg, K; Ouraou, A; Oyanguren, A; Paganoni, M; Paiano, S; Palacios, J P; Palka, H; Papadopoulou, T D; Pape, L; Parkes, C; Parodi, F; Parzefall, U; Passeri, A; Passon, O; Peralta, L; Perepelitsa, V F; Perrotta, A; Petrolini, A; Piedra, J; Pieri, L; Pierre, F; Pimenta, M; Piotto, E; Podobnik, T; Poireau, V; Pol, M E; Polok, G; Poropat, P; Pozdnyakov, V; Pukhaeva, N; Pullia, Antonio; Rames, J; Ramler, L; Read, A; Rebecchi, P; Rehn, J; Reid, D; Reinhardt, R; Renton, P B; Richard, F; Rídky, J; Rivero, M; Rodríguez, D; Romero, A; Ronchese, P; Rosenberg, E I; Roudeau, Patrick; Rovelli, T; Ruhlmann-Kleider, V; Ryabtchikov, D; Sadovskii, A; Salmi, L; Salt, J; Savoy-Navarro, A; Schwickerath, U; Segar, A; Sekulin, R L; Siebel, M; Sissakian, A N; Smadja, G; Smirnova, O G; Sokolov, A; Sopczak, A; Sosnowski, R; Spassoff, Tz; Stanitzki, M; Stavitski, I; Stocchi, A; Strauss, J; Stugu, B; Szczekowski, M; Szeptycka, M; Szumlak, T; Tabarelli de Fatis, T; Taffard, A C; Tegenfeldt, F; Timmermans, J; Tinti, N; Tkatchev, L G; Tobin, M; Todorovova, S; Tomaradze, A G; Tomé, B; Tonazzo, A; Tortosa, P; Travnicek, P; Treille, D; Trischuk, W; Tristram, G; Trochimczuk, M; Troncon, C; Turluer, M L; Tyapkin, I A; Tyapkin, P; Tyndel, M; Tzamarias, S; Uvarov, V; Valenti, G; van Dam, P; Van Eldik, J; Van Lysebetten, A; Van Remortel, N; Van Vulpen, I B; Vegni, G; Veloso, F; Venus, W A; Verbeure, F; Verdier, P; Verzi, V; Vilanova, D; Vitale, L; Vrba, V; Wahlen, H; Washbrook, A J; Weilhammer, Peter; Weiser, C; Wicke, D; Wickens, J H; Wilkinson, G; Winter, M; Witek, M; Yushchenko, O P; Zalewska-Bak, A; Zalewski, Piotr; Zavrtanik, D; Zimin, N I; Zinchenko, A I; Zupan, M

    2004-01-01

    The standard method used for tagging b-hadrons in the DELPHI experiment at the CERN LEP Collider is discussed in detail. The main ingredient of b-tagging is the impact parameters of tracks, which relies mostly on the vertex detector. Additional information, such as the mass of particles associated to a secondary vertex, significantly improves the selection efficiency and the background suppression. The paper describes various discriminating variables used for the tagging and the procedure of their combination. In addition, applications of b-tagging to some physics analyses, which depend crucially on the performance and reliability of b-tagging, are described briefly.

  17. Efficient expression of a soluble lipid transfer protein (LTP) of Platanus orientalis using short peptide tags and structural comparison with the natural form.

    Science.gov (United States)

    Salari, Farhad; Vahedi, Fatemeh; Chamani, Jamshidkhan; Varasteh, Abdolreza; Ketabdar, Hanieh; Sankian, Mojtaba

    2015-01-01

    Successful recombinant allergen-based immunotherapy has drawn a great deal of attention to use recombinant allergens for new therapeutic and/or diagnostic strategies. The Escherichia coli expression system is frequently used to produce recombinant allergens; however, protein expression in E. coli often results in inclusion bodies. Here, we focused on the expression of two recombinant soluble forms of Pla or 3 using solubility-enhancing peptide tags, human immune deficiency virus type 1 transactivator of transcription core domain and poly-arginine-lysine: rTAT-Pla or 3 and rPoly-Arg-Lys-Pla or 3. Structural characteristics and IgE reactivity of purified recombinant proteins were compared with natural Pla or 3 (nPla or 3) isolated from Platanus orientalis using circular dichroism spectra, fluorescence spectroscopy, and immunoblotting. Likewise, intrinsic viscosity and Stokes radius of the natural and recombinant Pla or 3 allergens were determined to analyze structural compactness in aqueous media. The results indicate high-level solubility and efficient expression of the fusion proteins (rTAT-Pla or 3 and rPoly-Arg-Lys-Pla or 3) compared with the wild-type recombinant. Furthermore, the similar structural characteristics and IgE-binding activities of the fusion proteins to nPla or 3 provide a promising tool for allergy diagnosis and treatment. © 2014 International Union of Biochemistry and Molecular Biology, Inc.

  18. A simultaneous measurement of the $b$-tagging efficiency scale factor and the $t\\bar{t}$ Production Cross Section at the Collider Detector at Fermilab

    Energy Technology Data Exchange (ETDEWEB)

    Hussain, Nazim; /McGill U.

    2011-07-01

    The ability to compare results between Monte Carlo and data is imperative in modern experimental high-energy physics analyses. The b-tagging efficiency Scale Factor (SF) allows for an accurate comparison of b quark identification in data samples and Monte Carlo. This thesis presents a simultaneous measurement of the SF for the SecVtx algorithm and the t{bar t} production cross section using 5.6 fb{sup -1} of p{bar p} collision data at {radical}s = 1.96 TeV collected by the Collider Detector at Fermilab (CDF) experiment. The t{bar t} cross section was measured to be 7.26 {+-} 0.47 pb, consistent with prior CDF analyses. The tight SF value was measured to be 0.925 {+-} 0.032 and the loose SF value was measured at 0.967 {+-} 0.033. These are the most precise SF SecVtx measurements to be performed at CDF to date.

  19. Hexagonal boron nitride neutron detectors with high detection efficiencies

    Science.gov (United States)

    Maity, A.; Grenadier, S. J.; Li, J.; Lin, J. Y.; Jiang, H. X.

    2018-01-01

    Neutron detectors fabricated from 10B enriched hexagonal boron nitride (h-10BN or h-BN) epilayers have demonstrated the highest thermal neutron detection efficiency among solid-state neutron detectors to date at about 53%. In this work, photoconductive-like vertical detectors with a detection area of 1 × 1 mm2 were fabricated from 50 μm thick free-standing h-BN epilayers using Ni/Au and Ti/Al bilayers as ohmic contacts. Leakage currents, mobility-lifetime (μτ) products under UV photoexcitation, and neutron detection efficiencies have been measured for a total of 16 different device configurations. The results have unambiguously identified that detectors incorporating the Ni/Au bilayer on both surfaces as ohmic contacts and using the negatively biased top surface for neutron irradiation are the most desired device configurations. It was noted that high growth temperatures of h-10BN epilayers on sapphire substrates tend to yield a higher concentration of oxygen impurities near the bottom surface, leading to a better device performance by the chosen top surface for irradiation than by the bottom. Preferential scattering of oxygen donors tends to reduce the mobility of holes more than that of electrons, making the biasing scheme with the ability of rapidly extracting holes at the irradiated surface while leaving the electrons to travel a large average distance inside the detector at a preferred choice. When measured against a calibrated 6LiF filled micro-structured semiconductor neutron detector, it was shown that the optimized configuration has pushed the detection efficiency of h-BN neutron detectors to 58%. These detailed studies also provided a better understanding of growth-mediated impurities in h-BN epilayers and their effects on the charge collection and neutron detection efficiencies.

  20. Yellowtail Tagging Data (MRDBS)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Yellowtail Flounder Tagging Program began in 2003 and works with commercial fishermen to tag and release yellowtaiI flounder with pink and yellow disc tags or...

  1. Efficient image duplicated region detection model using sequential block clustering

    Czech Academy of Sciences Publication Activity Database

    Sekeh, M. A.; Maarof, M. A.; Rohani, M. F.; Mahdian, Babak

    2013-01-01

    Roč. 10, č. 1 (2013), s. 73-84 ISSN 1742-2876 Institutional support: RVO:67985556 Keywords : Image forensic * Copy–paste forgery * Local block matching Subject RIV: IN - Informatics, Computer Science Impact factor: 0.986, year: 2013 http://library.utia.cas.cz/separaty/2013/ZOI/mahdian-efficient image duplicated region detection model using sequential block clustering.pdf

  2. Development of a single-antigen magnetic bead assay (SAMBA) for the sensitive detection of HPA-1a alloantibodies using tag-engineered recombinant soluble β3 integrin.

    Science.gov (United States)

    Skaik, Younis; Battermann, Anja; Hiller, Oliver; Meyer, Oliver; Figueiredo, Constanca; Salama, Abdulgabar; Blasczyk, Rainer

    2013-05-31

    Timely and accurate testing for human platelet antigen 1a (HPA-1a) alloantibodies is vital for clinical diagnosis of neonatal alloimmune thrombocytopenia (NAIT). Current antigen-specific assays used for the detection of HPA-1 alloantibodies are technically very complex and cumbersome for most diagnostic laboratories. Hence, we designed and applied recombinant soluble (rs) β3 integrins displaying HPA-1a or HPA-1b epitopes for the development of a single-antigen magnetic bead assay (SAMBA). Soluble HPA-1a and HPA-1b were produced recombinantly in human embryonic kidney 293 (HEK293) cells and differentially tagged. The recombinant soluble proteins were then immobilized onto paramagnetic beads and used for analysis of HPA-1 alloantibodies by enzyme-linked immunosorbent assay (ELISA). HPA-1a serum samples (n=7) from NAIT patients, inert sera and sera containing non-HPA-1a antibodies were used to evaluate the sensitivity and specificity of the SAMBA. Fusion of V5-His or GS-SBP-His tags to the rsβ3 integrins resulted in high-yield expression. SAMBA was able to detect all HPA-1a and -1b alloantibodies recognized by monoclonal antibody-specific immobilization of platelet antigens assay (MAIPA). No cross-reactions between the sera were observed. Two out of seven of the HPA-1a alloantibody-containing sera demonstrated weak to moderate reactivity in MAIPA but strong signals in SAMBA. SAMBA provides a very reliable method for the detection of HPA-1 antibodies with high specificity and sensitivity. This simple and rapid assay can be adapted for use in any routine laboratory and can be potentially adapted for use on automated systems. Copyright © 2013 Elsevier B.V. All rights reserved.

  3. Some Fundamental Limits on SAW RFID Tag Information Capacity and Collision Resolution

    Science.gov (United States)

    Barton, Richard J.

    2013-01-01

    In this paper, we apply results from multi-user information theory to study the limits of information capacity and collision resolution for SAW RFID tags. In particular, we derive bounds on the achievable data rate per tag as a function of fundamental parameters such as tag time-bandwidth product, tag signal-to-noise ratio (SNR), and number of tags in the environment. We also discuss the implications of these bounds for tag waveform design and tag interrogation efficiency

  4. An efficient voice activity detection algorithm by combining statistical model and energy detection

    Science.gov (United States)

    Wu, Ji; Zhang, Xiao-Lei

    2011-12-01

    In this article, we present a new voice activity detection (VAD) algorithm that is based on statistical models and empirical rule-based energy detection algorithm. Specifically, it needs two steps to separate speech segments from background noise. For the first step, the VAD detects possible speech endpoints efficiently using the empirical rule-based energy detection algorithm. However, the possible endpoints are not accurate enough when the signal-to-noise ratio is low. Therefore, for the second step, we propose a new gaussian mixture model-based multiple-observation log likelihood ratio algorithm to align the endpoints to their optimal positions. Several experiments are conducted to evaluate the proposed VAD on both accuracy and efficiency. The results show that it could achieve better performance than the six referenced VADs in various noise scenarios.

  5. An efficient voice activity detection algorithm by combining statistical model and energy detection

    Directory of Open Access Journals (Sweden)

    Wu Ji

    2011-01-01

    Full Text Available Abstract In this article, we present a new voice activity detection (VAD algorithm that is based on statistical models and empirical rule-based energy detection algorithm. Specifically, it needs two steps to separate speech segments from background noise. For the first step, the VAD detects possible speech endpoints efficiently using the empirical rule-based energy detection algorithm. However, the possible endpoints are not accurate enough when the signal-to-noise ratio is low. Therefore, for the second step, we propose a new gaussian mixture model-based multiple-observation log likelihood ratio algorithm to align the endpoints to their optimal positions. Several experiments are conducted to evaluate the proposed VAD on both accuracy and efficiency. The results show that it could achieve better performance than the six referenced VADs in various noise scenarios.

  6. Measurement of the Photoelectron Detection Efficiency of the HPD Anode

    CERN Document Server

    Carson, L; Soler, P

    2009-01-01

    This paper reports on measurements carried out on the Hybrid Photon Detectors (HPDs) of the LHCb RICH detectors. The purpose of these tests is to determine the photoelectron detection efficiency $\\eta$ of the HPD anode. Knowledge of $\\eta$ is required for an accurate simulation of the RICH detectors. It is found that this efficiency is $(93.3\\pm0.7)\\%$ for a 50 ns digital readout window, and $(87.9\\pm1.4)\\%$ for a 25 ns digital readout window. The 25 ns result exceeds the LHCb-RICH requirement of 85\\%, and is in agreement both with direct $\\eta$ measurements using preseries HPDs, and with indirect measurements from testbeams using preseries and production HPDs.

  7. Efficient RPG detection in noisy 3D image data

    Science.gov (United States)

    Pipitone, Frank

    2011-06-01

    We address the automatic detection of Ambush weapons such as rocket propelled grenades (RPGs) from range data which might be derived from multiple camera stereo with textured illumination or by other means. We describe our initial work in a new project involving the efficient acquisition of 3D scene data as well as discrete point invariant techniques to perform real time search for threats to a convoy. The shapes of the jump boundaries in the scene are exploited in this paper, rather than on-surface points, due to the large error typical of depth measurement at long range and the relatively high resolution obtainable in the transverse direction. We describe examples of the generation of a novel range-scaled chain code for detecting and matching jump boundaries.

  8. Wave study of compound eyes for efficient infrared detection

    Science.gov (United States)

    Kilinc, Takiyettin Oytun; Hayran, Zeki; Kocer, Hasan; Kurt, Hamza

    2017-08-01

    Improving sensitivity in the infrared spectrum is a challenging task. Detecting infrared light over a wide bandwidth and at low power consumption is very important. Novel solutions can be acquired by mimicking biological eyes such as compound eye with many individual lenses inspired from the nature. The nature provides many ingenious approaches of sensing and detecting the surrounding environment. Even though compound eye consists of small optical units, it can detect wide-angle electromagnetic waves and it has high transmission and low reflection loss. Insects have eyes that are superior compared to human eyes (single-aperture eyes) in terms of compactness, robustness, wider field of view, higher sensitivity of light intensity and being cheap vision systems. All these desired properties are accompanied by an important drawback: lower spatial resolution. The first step to investigate the feasibility of bio-inspired optics in photodetectors is to perform light interaction with the optical system that gather light and detect it. The most common method used in natural vision systems is the ray analysis. Light wave characteristics are not taken into consideration in such analyses, such as the amount of energy at the focal point or photoreceptor site, the losses caused by reflection at the interfaces and absorption cannot be investigated. In this study, we present a bio-inspired optical detection system investigated by wave analysis. We numerically model the wave analysis based on Maxwell equations from the viewpoint of efficient light detection and revealing the light propagation after intercepting the first interface of the eye towards the photoreceptor site.

  9. Electrochemical immunoassay for thyroxine detection using cascade catalysis as signal amplified enhancer and multi-functionalized magnetic graphene sphere as signal tag.

    Science.gov (United States)

    Han, Jing; Zhuo, Ying; Chai, Yaqin; Yu, Yanqing; Liao, Ni; Yuan, Ruo

    2013-08-06

    This paper constructed a reusable electrochemical immunosensor for the detection of thyroxine at an ultralow concentration using cascade catalysis of cytochrome c (Cyt c) and glucose oxidase (GOx) as signal amplified enhancer. It is worth pointing out that numerous Cyt c and GOx were firstly carried onto the double-stranded DNA polymers based on hybridization chain reaction (HCR), and then the amplified responses could be achieved by cascade catalysis of Cyt c and GOx recycling with the help of glucose. Moreover, multi-functionalized magnetic graphene sphere was synthesized and used as signal tag, which not only exhibited good mechanical properties, large surface area and an excellent electron transfer rate of graphene, but also possessed excellent redox activity and desirable magnetic property. With a sandwich-type immunoreaction, the proposed cascade catalysis amplification strategy could greatly enhance the sensitivity for the detection of thyroxine. Under the optimal conditions, the immunosensor showed a wide linear ranged from 0.05pg mL(-1) to 5ng mL(-1) and a low detection limit down to 15fg mL(-1). Importantly, the proposed method offers promise for reproducible and cost-effective analysis of biological samples. Copyright © 2013 Elsevier B.V. All rights reserved.

  10. Fusion of local and global features for efficient object detection

    Science.gov (United States)

    Le, Duy-Dinh; Satoh, Shin'ichi

    2005-02-01

    In this paper, we propose a two-stage method for efficient object detection that combines full advantages of AdaBoost and SVM to achieve a reasonable balance in both training and classification speed. In the first stage, we use Haar wavelet features and AdaBoost to train a cascade of classifiers for quick and efficient rejection. This cascade of classifiers consists of simple-to-complex classifiers that allow adapting to complexity of input patterns, rejects almost 90%-99% non-object patterns rapidly. Hard patterns, object-like patterns, which are passed through the first stage, will be classified by the second stage which uses a non linear SVM-based classifier with pixel-based global features. The nonlinear SVM classifier is robust enough in order to reach high performance. We have investigated our proposed method to detect different kinds of objects such as face and facial features like eye and mouth regions. In training, our system is roughly 25 times faster than the system trained by AdaBoost. In running, the experimental results show 1,000 times faster than SVM based method and slightly slower than AdaBoost based method with a comparable accuracy.

  11. Calibration and neutron detection efficiency in Double Chooz

    Energy Technology Data Exchange (ETDEWEB)

    Almazan, Helena; Buck, Christian; Haser, Julia; Lindner, Manfred [MPIK, Heidelberg (Germany); Collaboration: Double Chooz-Collaboration

    2016-07-01

    As an intense and pure source of low energy electron antineutrinos, nuclear reactors are one of the most powerful tools to analyse the neutrino oscillations. The Double Chooz experiment aims for a precise determination of the neutrino mixing angle θ{sub 13} with the new data from the near detector. In order to reach this precision, a high and accurately known detection efficiency of the inverse beta decay (IBD) signal - the antineutrino interaction - is required. Several methods are available for detector calibration. Cosmic muons and spallation neutron captures are some examples of natural sources that are used. Furthermore, signals created by artificial sources contribute to the calibration with well defined classes of events. LED Light Injection systems in the Inner Detector and the Inner Veto are used to measure PMT gains and time responses. Radioactive sources deployed inside the detector are used to determine the energy scale and the detector stability. The {sup 252}Cf source plays an important role in the detector calibration. In the spontaneous fissions of this isotope neutrons are produced with high multiplicity. An analysis of the neutron interactions in the scintillator can be used to estimate the detection efficiency of the delayed coincidence signal of the IBD reaction. New results from recent calibration campaigns will be presented, providing a crucial input for reactor antineutrino analysis with two detectors.

  12. Depth- and range-dependent variation in the performance of aquatic telemetry systems: understanding and predicting the susceptibility of acoustic tag-receiver pairs to close proximity detection interference.

    Science.gov (United States)

    Scherrer, Stephen R; Rideout, Brendan P; Giorli, Giacomo; Nosal, Eva-Marie; Weng, Kevin C

    2018-01-01

    Passive acoustic telemetry using coded transmitter tags and stationary receivers is a popular method for tracking movements of aquatic animals. Understanding the performance of these systems is important in array design and in analysis. Close proximity detection interference (CPDI) is a condition where receivers fail to reliably detect tag transmissions. CPDI generally occurs when the tag and receiver are near one another in acoustically reverberant settings. Here we confirm transmission multipaths reflected off the environment arriving at a receiver with sufficient delay relative to the direct signal cause CPDI. We propose a ray-propagation based model to estimate the arrival of energy via multipaths to predict CPDI occurrence, and we show how deeper deployments are particularly susceptible. A series of experiments were designed to develop and validate our model. Deep (300 m) and shallow (25 m) ranging experiments were conducted using Vemco V13 acoustic tags and VR2-W receivers. Probabilistic modeling of hourly detections was used to estimate the average distance a tag could be detected. A mechanistic model for predicting the arrival time of multipaths was developed using parameters from these experiments to calculate the direct and multipath path lengths. This model was retroactively applied to the previous ranging experiments to validate CPDI observations. Two additional experiments were designed to validate predictions of CPDI with respect to combinations of deployment depth and distance. Playback of recorded tags in a tank environment was used to confirm multipaths arriving after the receiver's blanking interval cause CPDI effects. Analysis of empirical data estimated the average maximum detection radius (AMDR), the farthest distance at which 95% of tag transmissions went undetected by receivers, was between 840 and 846 m for the deep ranging experiment across all factor permutations. From these results, CPDI was estimated within a 276.5 m radius of the

  13. A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile Red

    Science.gov (United States)

    Maes, Thomas; Jessop, Rebecca; Wellner, Nikolaus; Haupt, Karsten; Mayes, Andrew G.

    2017-03-01

    A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

  14. Detecting emerging trends and country specializations in Energy Efficiency

    Energy Technology Data Exchange (ETDEWEB)

    De Filippo, D.; Pandiella Dominique, A.

    2016-07-01

    The sector of Energy Efficiency is key in the development of a country. Energy saving means cost savings and this affects GDP growth and employment. In addition, there are environmental benefits such as savings in the use of natural resources or reducing carbon emissions. If we also take into account the deduction of foreign energy dependence, it is noted that it is a sector of vital economic and strategic importance. Proof of this is that energy efficiency is a priority policy in science and technology agenda of most countries (OECD, 2011). Although it is an area of global concern, the particularities of each context make both the issues and the areas of scientific and technological activity can be considerably different from one country to another. To study these peculiarities, scientific publications in international databases were analyzed. These outputs are a good indicator of the intensity and specialization of scientific and technological activity of countries. Also, the characteristics of energy efficiency sector -such as the crosscutting and interdisciplinarity- make it an attractive object to be approached from a bibliometric perspective. This methodological approach was used because bibliometrics has become one of the central models to analyze measure and evaluate different aspects of the scientific activity (Callon, Courtial, & Penan, 1995). Among its most frequent uses are, the analysis of scientific productivity (researchers, groups, institutions, disciplines or countries), and the study of its impact on the scientific community. However, it has had more innovative applications, as the detection of new research fronts, the study of collaborative networking between different institutional actors and the identification of niche of research. Given these considerations, the aim of this work is to show the results of a study in which we analyze scientific activity in energy efficiency through bibliometric indicators. The bibliometric research, constructed

  15. Efficient 1.8 t high-efficiency scintillation counter for neutron detection

    International Nuclear Information System (INIS)

    Bezrukov, L.B.; Enikeev, R.I.; Korchagin, V.B.; Ryazhskaya, O.G.

    1975-01-01

    Specific features and the principle of operation of a liquid scintillation counter designed for the detection of neutrons generated in showers due to inelastic scattering of cosmic ray muons are described. The detector consists of three rows of scintillation counters-modules by four modules in every row. Each module is scanned by two photomultipliers of the 49 type that extend to the windows out of plexiglass. The counter is filled with liquid organic scintillator based on white spirit with additives of 1g/1 PPO (2.5-diphenyloxazole) and 0.03 g/1 POPOP (1.4-di-[2-(5-phenyloxazole)]benzene) with 2.4 g/l Gd octoate dissolved in it. The volume of the counter is 1800 l, the area is 2 m 2 , it contains 1.8 t of organic scintillator. The general view of the counter is shown, the block-diagram for measuring neutron detection efficiency is presented. The efficiency of neutron detection measured with the aid of 252 Cf placed in the centre of the counter is (83 +- 1)%

  16. Remote monitoring of fish in small streams: A unified approach using PIT tags

    Science.gov (United States)

    Zydlewski, G.B.; Horton, G.; Dubreuil, T.; Letcher, B.; Casey, S.; Zydlewski, Joseph D.

    2006-01-01

    Accurate assessments of fish populations are often limited by re-observation or recapture events. Since the early 1990s, passive integrated transponders (PIT tags) have been used to understand the biology of many fish species. Until recently, PIT applications in small streams have been limited to physical recapture events. To maximize recapture probability, we constructed PIT antenna arrays in small streams to remotely detect individual fish. Experiences from two different laboratories (three case studies) allowed us to develop a unified approach to applying PIT technology for enhancing data assessments. Information on equipment, its installation, tag considerations, and array construction is provided. Theoretical and practical definitions are introduced to standardize metrics for assessing detection efficiency. We demonstrate how certain conditions (stream discharge, vibration, and ambient radio frequency noise) affect the detection efficiency and suggest that by monitoring these conditions, expectations of efficiency can be modified. We emphasize the importance of consistently estimating detection efficiency for fisheries applications.

  17. Quantitative comparison of DNA detection by GFP-lac repressor tagging, fluorescence in situ hybridization and immunostaining

    Directory of Open Access Journals (Sweden)

    Rohr Karl

    2007-12-01

    Full Text Available Abstract Background GFP-fusion proteins and immunostaining are methods broadly applied to investigate the three-dimensional organization of cells and cell nuclei, the latter often studied in addition by fluorescence in situ hybridization (FISH. Direct comparisons of these detection methods are scarce, however. Results We provide a quantitative comparison of all three approaches. We make use of a cell line that contains a transgene array of lac operator repeats which are detected by GFP-lac repressor fusion proteins. Thus we can detect the same structure in individual cells by GFP fluorescence, by antibodies against GFP and by FISH with a probe against the transgene array. Anti-GFP antibody detection was repeated after FISH. Our results show that while all four signals obtained from a transgene array generally showed qualitative and quantitative similarity, they also differed in details. Conclusion Each of the tested methods revealed particular strengths and weaknesses, which should be considered when interpreting respective experimental results. Despite the required denaturation step, FISH signals in structurally preserved cells show a surprising similarity to signals generated before denaturation.

  18. Efficiently detecting outlying behavior in video-game players

    Directory of Open Access Journals (Sweden)

    Young Bin Kim

    2015-12-01

    Full Text Available In this paper, we propose a method for automatically detecting the times during which game players exhibit specific behavior, such as when players commonly show excitement, concentration, immersion, and surprise. The proposed method detects such outlying behavior based on the game players’ characteristics. These characteristics are captured non-invasively in a general game environment. In this paper, cameras were used to analyze observed data such as facial expressions and player movements. Moreover, multimodal data from the game players (i.e., data regarding adjustments to the volume and the use of the keyboard and mouse was used to analyze high-dimensional game-player data. A support vector machine was used to efficiently detect outlying behaviors. We verified the effectiveness of the proposed method using games from several genres. The recall rate of the outlying behavior pre-identified by industry experts was approximately 70%. The proposed method can also be used for feedback analysis of various interactive content provided in PC environments.

  19. An efficient community detection algorithm using greedy surprise maximization

    International Nuclear Information System (INIS)

    Jiang, Yawen; Jia, Caiyan; Yu, Jian

    2014-01-01

    Community detection is an important and crucial problem in complex network analysis. Although classical modularity function optimization approaches are widely used for identifying communities, the modularity function (Q) suffers from its resolution limit. Recently, the surprise function (S) was experimentally proved to be better than the Q function. However, up until now, there has been no algorithm available to perform searches to directly determine the maximal surprise values. In this paper, considering the superiority of the S function over the Q function, we propose an efficient community detection algorithm called AGSO (algorithm based on greedy surprise optimization) and its improved version FAGSO (fast-AGSO), which are based on greedy surprise optimization and do not suffer from the resolution limit. In addition, (F)AGSO does not need the number of communities K to be specified in advance. Tests on experimental networks show that (F)AGSO is able to detect optimal partitions in both simple and even more complex networks. Moreover, algorithms based on surprise maximization perform better than those algorithms based on modularity maximization, including Blondel–Guillaume–Lambiotte–Lefebvre (BGLL), Clauset–Newman–Moore (CNM) and the other state-of-the-art algorithms such as Infomap, order statistics local optimization method (OSLOM) and label propagation algorithm (LPA). (paper)

  20. A Phosphorylation Tag for Uranyl Mediated Protein Purification and Photo Assisted Tag Removal

    DEFF Research Database (Denmark)

    Zhang, Qiang; Jørgensen, Thomas. J. D.; Nielsen, Peter E

    2014-01-01

    of enzymes available for this purpose. In the present study, we demonstrate the utility of the divalent uranyl ion in a new procedure for protein purification and tag removal. By employment of a GFP (green florescence protein) recombinant protein we show that uranyl binding to a phosphorylated C-terminal tag......Most protein purification procedures include an affinity tag fused to either the N or C-terminal end of the protein of interest as well as a procedure for tag removal. Tag removal is not straightforward and especially tag removal from the C-terminal end is a challenge due to the characteristics...... enables target protein purification from an E. coli extract by immobilized uranyl affinity chromatography. Subsequently, the tag can be efficiently removed by UV-irradiation assisted uranyl photocleavage. We therefore suggest that the divalent uranyl ion (UO22+) may provide a dual function in protein...

  1. CRISPR/Cas9 allows efficient and complete knock-in of a destabilization domain-tagged essential protein in a human cell line, allowing rapid knockdown of protein function.

    Directory of Open Access Journals (Sweden)

    Arnold Park

    Full Text Available Although modulation of protein levels is an important tool for study of protein function, it is difficult or impossible to knockdown or knockout genes that are critical for cell growth or viability. For such genes, a conditional knockdown approach would be valuable. The FKBP protein-based destabilization domain (DD-tagging approach, which confers instability to the tagged protein in the absence of the compound Shield-1, has been shown to provide rapid control of protein levels determined by Shield-1 concentration. Although a strategy to knock-in DD-tagged protein at the endogenous loci has been employed in certain parasite studies, partly due to the relative ease of knock-in as a result of their mostly haploid lifecycles, this strategy has not been demonstrated in diploid or hyperploid mammalian cells due to the relative difficulty of achieving complete knock-in in all alleles. The recent advent of CRISPR/Cas9 homing endonuclease-mediated targeted genome cleavage has been shown to allow highly efficient homologous recombination at the targeted locus. We therefore assessed the feasibility of using CRISPR/Cas9 to achieve complete knock-in to DD-tag the essential gene Treacher Collins-Franceschetti syndrome 1 (TCOF1 in human 293T cells. Using a double antibiotic selection strategy to select clones with at least two knock-in alleles, we obtained numerous complete knock-in clones within three weeks of initial transfection. DD-TCOF1 expression in the knock-in cells was Shield-1 concentration-dependent, and removal of Shield-1 resulted in destabilization of DD-TCOF1 over the course of hours. We further confirmed that the tagged TCOF1 retained the nucleolar localization of the wild-type untagged protein, and that destabilization of DD-TCOF1 resulted in impaired cell growth, as expected for a gene implicated in ribosome biogenesis. CRISPR/Cas9-mediated homologous recombination to completely knock-in a DD tag likely represents a generalizable and

  2. CRISPR/Cas9 allows efficient and complete knock-in of a destabilization domain-tagged essential protein in a human cell line, allowing rapid knockdown of protein function.

    Science.gov (United States)

    Park, Arnold; Won, Sohui T; Pentecost, Mickey; Bartkowski, Wojciech; Lee, Benhur

    2014-01-01

    Although modulation of protein levels is an important tool for study of protein function, it is difficult or impossible to knockdown or knockout genes that are critical for cell growth or viability. For such genes, a conditional knockdown approach would be valuable. The FKBP protein-based destabilization domain (DD)-tagging approach, which confers instability to the tagged protein in the absence of the compound Shield-1, has been shown to provide rapid control of protein levels determined by Shield-1 concentration. Although a strategy to knock-in DD-tagged protein at the endogenous loci has been employed in certain parasite studies, partly due to the relative ease of knock-in as a result of their mostly haploid lifecycles, this strategy has not been demonstrated in diploid or hyperploid mammalian cells due to the relative difficulty of achieving complete knock-in in all alleles. The recent advent of CRISPR/Cas9 homing endonuclease-mediated targeted genome cleavage has been shown to allow highly efficient homologous recombination at the targeted locus. We therefore assessed the feasibility of using CRISPR/Cas9 to achieve complete knock-in to DD-tag the essential gene Treacher Collins-Franceschetti syndrome 1 (TCOF1) in human 293T cells. Using a double antibiotic selection strategy to select clones with at least two knock-in alleles, we obtained numerous complete knock-in clones within three weeks of initial transfection. DD-TCOF1 expression in the knock-in cells was Shield-1 concentration-dependent, and removal of Shield-1 resulted in destabilization of DD-TCOF1 over the course of hours. We further confirmed that the tagged TCOF1 retained the nucleolar localization of the wild-type untagged protein, and that destabilization of DD-TCOF1 resulted in impaired cell growth, as expected for a gene implicated in ribosome biogenesis. CRISPR/Cas9-mediated homologous recombination to completely knock-in a DD tag likely represents a generalizable and efficient strategy to

  3. Efficient expression of codon-adapted affinity tagged super folder green fluorescent protein for synchronous protein localization and affinity purification studies in Tetrahymena thermophila.

    Science.gov (United States)

    Yilmaz, Gürkan; Arslanyolu, Muhittin

    2015-03-25

    A superior Green Fluorescent Protein (GFP) mutant, known as superfolder GFP (sfGFP), is more soluble, faster folding, and is the brightest of the known GFP mutants. This study aimed to create a codon-adapted sfGFP tag (TtsfGFP) for simultaneous protein localization and affinity purification in Tetrahymena thermophila. In vivo fluorescence spectroscopic analyses of clones carrying a codon-adapted and 6 × His tagged TtsfGFP cassette showed approximately 2-4-fold increased fluorescence emission compared with the control groups at 3 h. Fluorescence microscopy also revealed that TtsfGFP reached its emission maxima at 100 min, which was much earlier than controls expressing EGFP and sfGFP (240 min). A T. thermophila ATP-dependent DNA ligase domain containing hypothetical gene (H) was cloned into the 3' end of 6 × His-TtsfGFP to assess the affinity/localization dual tag feature. Fluorescence microscopy of the 6 × His-TtsfGFP-H clone confirmed its localization in the macro- and micronucleus of vegetative T. thermophila. Simultaneous affinity purification of TtsfGFP and TtsfGFP-H with Ni-NTA beads was feasible, as shown by Ni-NTA purified proteins analysis by SDS-PAGE and western blotting. We successfully codon adapted the N-terminal 6 × His-TtsfGFP tag and showed that it could be used for protein localization and affinity purification simultaneously in T. thermophila. We believe that this dual tag will advance T. thermophila studies by providing strong visual traceability of the target protein in vivo and in vitro during recombinant production of heterologous and homologous proteins.

  4. An Expressed Sequence Tag collection from the male antennae of the Noctuid moth Spodoptera littoralis: a resource for olfactory and pheromone detection research

    Directory of Open Access Journals (Sweden)

    Maïbèche-Coisné Martine

    2011-01-01

    Full Text Available Abstract Background Nocturnal insects such as moths are ideal models to study the molecular bases of olfaction that they use, among examples, for the detection of mating partners and host plants. Knowing how an odour generates a neuronal signal in insect antennae is crucial for understanding the physiological bases of olfaction, and also could lead to the identification of original targets for the development of olfactory-based control strategies against herbivorous moth pests. Here, we describe an Expressed Sequence Tag (EST project to characterize the antennal transcriptome of the noctuid pest model, Spodoptera littoralis, and to identify candidate genes involved in odour/pheromone detection. Results By targeting cDNAs from male antennae, we biased gene discovery towards genes potentially involved in male olfaction, including pheromone reception. A total of 20760 ESTs were obtained from a normalized library and were assembled in 9033 unigenes. 6530 were annotated based on BLAST analyses and gene prediction software identified 6738 ORFs. The unigenes were compared to the Bombyx mori proteome and to ESTs derived from Lepidoptera transcriptome projects. We identified a large number of candidate genes involved in odour and pheromone detection and turnover, including 31 candidate chemosensory receptor genes, but also genes potentially involved in olfactory modulation. Conclusions Our project has generated a large collection of antennal transcripts from a Lepidoptera. The normalization process, allowing enrichment in low abundant genes, proved to be particularly relevant to identify chemosensory receptors in a species for which no genomic data are available. Our results also suggest that olfactory modulation can take place at the level of the antennae itself. These EST resources will be invaluable for exploring the mechanisms of olfaction and pheromone detection in S. littoralis, and for ultimately identifying original targets to fight against moth

  5. Efficiency of template banks for binary black-hole detection

    International Nuclear Information System (INIS)

    Cokelaer, Thomas; Babak, Stas; Sathyaprakash, B S

    2004-01-01

    In the framework of matched filtering theory, which is the most promising method for the detection of gravitational waves emitted by coalescing binaries, we report on the ability of a template bank to catch a simulated binary black-hole gravitational wave signal. If we suppose that the incoming signal waveform is known a priori, then both the (simulated) signal and the templates can be based on the same physical model and therefore the template bank can be optimal in the sense of Wiener filtering. This turns out to be true for the case of neutron star binaries but not necessarily for the black-hole case. When the templates and the signal are based on different physical models the detection bank may still remain efficient. Nonetheless, it might be a judicious choice to use a phenomenological template family such as the so-called BCV templates to catch all the different physical models. In the first part of that report, we illustrate in a non-exhaustive study, by using Monte Carlo simulations, the efficiency of a template bank based on the stationary phase approximation and show how it catches simulated signals based on the same physical model but fails to catch signals built using other models (Pade, EOB, ...) especially in the case of high mass binaries. In the second part, we construct a BCV-template bank and test its validity by injecting simulated signals based on different physical models such as the PN-approximants, Pade-approximant and the effective one-body method. We show that it is suitable for a search pipeline since it gives a match higher than 95% for all the different physical models. The range of individual mass which has been used is [3-20]M o-dot

  6. Efficient multiparty quantum key agreement with collective detection.

    Science.gov (United States)

    Huang, Wei; Su, Qi; Liu, Bin; He, Yuan-Hang; Fan, Fan; Xu, Bing-Jie

    2017-11-10

    As a burgeoning branch of quantum cryptography, quantum key agreement is a kind of key establishing processes where the security and fairness of the established common key should be guaranteed simultaneously. However, the difficulty on designing a qualified quantum key agreement protocol increases significantly with the increase of the number of the involved participants. Thus far, only few of the existing multiparty quantum key agreement (MQKA) protocols can really achieve security and fairness. Nevertheless, these qualified MQKA protocols are either too inefficient or too impractical. In this paper, an MQKA protocol is proposed with single photons in travelling mode. Since only one eavesdropping detection is needed in the proposed protocol, the qubit efficiency and measurement efficiency of it are higher than those of the existing ones in theory. Compared with the protocols which make use of the entangled states or multi-particle measurements, the proposed protocol is more feasible with the current technologies. Security and fairness analysis shows that the proposed protocol is not only immune to the attacks from external eavesdroppers, but also free from the attacks from internal betrayers.

  7. Satellite Tags- Hawaii EEZ

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Satellite tagging was implemented in 2013. Satellite tagging is conducted using a Dan Inject air rifle and deployment arrows designed by Wildlife Computers. Two...

  8. Donor Tag Game

    Science.gov (United States)

    ... Donor Community > Games > Donor Tag Game Donor Tag Game This feature requires version 6 or later of ... of Needles LGBTQ+ Donors Blood Donor Community SleevesUp Games Facebook Avatars and Badges Banners eCards Make a ...

  9. Cooperative Tagging Center (CTC)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Cooperative Tagging Center (CTC) began as the Cooperative Game Fish Tagging Program (GTP) at Woods Hole Oceanographic Institute (WHOI) in 1954. The GTP was...

  10. MCNP to study the BF3 detection efficiency

    International Nuclear Information System (INIS)

    Castro, Vinicius A.; Cavalieri, Tassio A.; Siqueira, Paulo T.D.; Fedorenko, Giuliana G.; Coelho, Paulo R.P.; Madi Filho, Tufic

    2011-01-01

    One of the main parameters to monitor on the employment of the Boron Neutron Capture Therapy (BNCT) is the thermal neutron flux. It can be performed by different techniques such as the activation analysis and the detection by a Boron Trifluoride detector (BF 3 ). BF 3 detector is a real time neutron flux detector which retrieves results in real time. It is however necessary to study the efficiency of the BF 3 detectors when they are exposed to fields of different neutron energy spectra. BF 3 is known to have high efficiency for thermal neutrons (with energy up to 0.5 eV) due the presence of 10 B atoms in the detector. However, one must also understand how this detector interacts with other neutron energy ranges (epithermal and fast). This work shows the experiment and a set of associated simulations carried out in order to evaluate the BF 3 detector efficiency dependence on neutron energy spectra. A set of experiments was conducted in which a BF 3 detector was submitted to different mixed fields (field containing gamma rays and neutrons). These fields were generated by the interposition of paraffin layers with distinct thicknesses between the Am-Be source and the BF 3 detector. The BF 3 detector responses were recorded according to the number of paraffin planes used. MCNP simulations were also performed to study the detector responses on such experimental conditions. It has been possible to achieve the intended goal of evaluating the BF 3 detector response to different mixed irradiation fields. (author)

  11. An Efficient Conflict Detection Algorithm for Packet Filters

    Science.gov (United States)

    Lee, Chun-Liang; Lin, Guan-Yu; Chen, Yaw-Chung

    Packet classification is essential for supporting advanced network services such as firewalls, quality-of-service (QoS), virtual private networks (VPN), and policy-based routing. The rules that routers use to classify packets are called packet filters. If two or more filters overlap, a conflict occurs and leads to ambiguity in packet classification. This study proposes an algorithm that can efficiently detect and resolve filter conflicts using tuple based search. The time complexity of the proposed algorithm is O(nW+s), and the space complexity is O(nW), where n is the number of filters, W is the number of bits in a header field, and s is the number of conflicts. This study uses the synthetic filter databases generated by ClassBench to evaluate the proposed algorithm. Simulation results show that the proposed algorithm can achieve better performance than existing conflict detection algorithms both in time and space, particularly for databases with large numbers of conflicts.

  12. Substantial prevalence of microdeletions of the Y-chromosome in infertile men with idiopathic azoospermia and oligozoospermia detected using a sequence-tagged site-based mapping strategy

    Energy Technology Data Exchange (ETDEWEB)

    Najmabadi, H.; Huang, V.; Bhasin, D. [Drew Univ. of Medicine and Science, Los Angeles, CA (United States)] [and others

    1996-04-01

    Genes on the long arm of Y (Yq), particularly within interval 6, are believed to play a critical role in human spermatogenesis. Cytogenetically detectable deletions of this region are associated with azoospermia in men, but are relatively uncommon. The objective of this study was to validate a sequence-tagged site (STS)-mapping strategy for the detection of Yq microdeletions and to use this method to determine the proportion of men with idiopathic azoospermia or severe oligozoospermia who carry microdeletions in Yq. STS mapping of a sufficiently large sample of infertile men should also help further localize the putative gene(s) involved in the pathogenesis of male infertility. Genomic DNA was extracted from peripheral leukocytes of 16 normal fertile men, 7 normal fertile women, 60 infertile men, and 15 patients with the X-linked disorder, ichthyosis. PCR primers were synthesized for 26 STSs that span Yq interval 6. None of the 16 normal men of known fertility had microdeletions. Seven normal fertile women failed to amplify any of the 26 STSs, providing evidence of their Y specificity. No microdeletions were detected in any of the 15 patients with ichthyosis. Of the 60 infertile men typed with 26 STSs, 11 (18%; 10 azoospermic and 1 oligozoospermic) failed to amplify 1 or more STS. Interestingly, 4 of the 11 patients had microdeletions in a region that is outside the Yq region from which the DAZ (deleted in azoospermia gene region) gene was cloned. In an additional 3 patients, microdeletions were present both inside and outside the DAZ region. The physical locations of these microdeletions provide further support for the concept that a gene(s) on Yq deletion interval 6 plays an important role in spermatogenesis. The presence of deletions that do not overlap with the DAZ region suggests that genes other than the DAZ gene may also be implicated in the pathogenesis of some subsets of male infertility. 48 refs., 2 figs., 2 tabs.

  13. Voltammetric Detection of S100B Protein Using His-Tagged Receptor Domains for Advanced Glycation End Products (RAGE Immobilized onto a Gold Electrode Surface

    Directory of Open Access Journals (Sweden)

    Edyta Mikuła

    2014-06-01

    Full Text Available In this work we report on an electrochemical biosensor for the determination of the S100B protein. The His-tagged VC1 domains of Receptors for Advanced Glycation End (RAGE products used as analytically active molecules were covalently immobilized on a monolayer of a thiol derivative of pentetic acid (DPTA complex with Cu(II deposited on a gold electrode surface. The recognition processes between the RAGE VC1 domain and the S100B protein results in changes in the redox activity of the DPTA-Cu(II centres which were measured by Osteryoung square-wave voltammetry (OSWV. In order to verify whether the observed analytical signal originates from the recognition process between the His6–RAGE VC1 domains and the S100B protein, the electrode modified with the His6–RAGE C2 and His6–RAGE VC1 deleted domains which have no ability to bind S100B peptides were applied. The proposed biosensor was quite sensitive, with a detection limit of 0.52 pM recorded in the buffer solution. The presence of diluted human plasma and 10 nM Aβ1-40 have no influence on the biosensor performance.

  14. Thermo-mechanical actuator-based miniature tagging module for localization in capsule endoscopy

    Science.gov (United States)

    Chandrappan, Jayakrishnan; Ruiqi, Lim; Su, Nandar; Yen Yi, Germaine Hoe; Vaidyanathan, Kripesh

    2011-04-01

    Capsule endoscopy is a frontline medical diagnostic tool for the gastro intestinal tract disorders. During diagnosis, efficient localization techniques are essential to specify a pathological area that may require further diagnosis or treatment. This paper presents the development of a miniature tagging module that relies on a novel concept to label the region of interest and has the potential to integrate with a capsule endoscope. The tagging module is a compact thermo-mechanical actuator loaded with a biocompatible micro tag. A low power microheater attached to the module serves as the thermal igniter for the mechanical actuator. At optimum temperature, the actuator releases the micro tag instantly and penetrates the mucosa layer of a GI tract, region of interest. Ex vivo animal trials are conducted to verify the feasibility of the tagging module concept. X-ray imaging is used to detect the location of the micro tag embedded in the GI tract wall. The method is successful, and radiopaque micro tags can provide valuable pre-operative position information on the infected area to facilitate further clinical procedures.

  15. Efficient Mining and Detection of Sequential Intrusion Patterns for Network Intrusion Detection Systems

    Science.gov (United States)

    Shyu, Mei-Ling; Huang, Zifang; Luo, Hongli

    In recent years, pervasive computing infrastructures have greatly improved the interaction between human and system. As we put more reliance on these computing infrastructures, we also face threats of network intrusion and/or any new forms of undesirable IT-based activities. Hence, network security has become an extremely important issue, which is closely connected with homeland security, business transactions, and people's daily life. Accurate and efficient intrusion detection technologies are required to safeguard the network systems and the critical information transmitted in the network systems. In this chapter, a novel network intrusion detection framework for mining and detecting sequential intrusion patterns is proposed. The proposed framework consists of a Collateral Representative Subspace Projection Modeling (C-RSPM) component for supervised classification, and an inter-transactional association rule mining method based on Layer Divided Modeling (LDM) for temporal pattern analysis. Experiments on the KDD99 data set and the traffic data set generated by a private LAN testbed show promising results with high detection rates, low processing time, and low false alarm rates in mining and detecting sequential intrusion detections.

  16. Sensor-based material tagging system

    International Nuclear Information System (INIS)

    Vercellotti, L.C.; Cox, R.W.; Ravas, R.J.; Schlotterer, J.C.

    1991-01-01

    Electronic identification tags are being developed for tracking material and personnel. In applying electronic identification tags to radioactive materials safeguards, it is important to measure attributes of the material to ensure that the tag remains with the material. The addition of a microcontroller with an on-board analog-to-digital converter to an electronic identification tag application-specific integrated-circuit has been demonstrated as means to provide the tag with sensor data. Each tag is assembled into a housing, which serves as a scale for measuring the weight of a paint-can-sized container and its contents. Temperature rise of the can above ambient is also measured, and a piezoelectric detector detects disturbances and immediately puts the tag into its alarm and beacon mode. Radiation measurement was also considered, but the background from nearby containers was found to be excessive. The sensor-based tagging system allows tracking of the material in cans as it is stored in vaults or is moved through the manufacturing process. The paper presents details of the sensor-based material tagging system and describes a demonstration system

  17. Evaluation of Fish Movements, Migration Patterns and Populations Abundance with Streamwidth PIT Tag Interrogation Systems, Final Report 2002.

    Energy Technology Data Exchange (ETDEWEB)

    Zydlewski, Gayle B.; Casey, Sean

    2003-02-01

    Two remote Streamwidth PIT tag Interrogation systems (SPIs) were operated continuously for over one year to test the feasibility of these systems for generating movement, migration, survival and smolt production estimates for salmonids. A total of 1,588 juvenile (< 100 mm FL) naturally produced salmonids (7 coho salmon, 482 cutthroat trout, and 1,099 steelhead) were PIT tagged above the upstream-most SPI (9 sites approximately 1 linear km each) in Fall 2001. Age at tagging for wild caught cutthroat and steelhead was 1 year. SPIs were operating before any PIT tagged fish were released in the creek. Over 390,000 detections were recorded from October 2001 to 31 July 2002. Efficiencies were site dependent, but overall detection efficiency for the creek was 97% with 95% confidence intervals of 91-100%. PIT tag detection efficiency ranged from 55-100% depending on the SPI and varied throughout the year with average efficiencies of 73% and 89%. SPI efficiency of PIT tag detection was not completely dependent on electronics noise levels or environmental conditions. Fish from all tagging locations were detected at the SPIs. Steelhead and cutthroat trout were primarily detected moving in the Spring (April-June) coincident with the anticipated smolt migration. Steelhead were also detected moving past SPIs at lower numbers in the Fall and Winter. Travel time between SPIs (downstream movement) was highly dependent on time of year. Travel time in the Spring was significantly faster (34.4 {+-} 7.0 hours) for all species than during any other time of year (763.1 {+-} 267.0 hours). Steelhead and cutthroat migrating in the Spring were the same age as those that did not migrate in the Spring. Peak of steelhead migration recorded at the two SPIs was 5/11 and 5/12 and the peak in the screw trap was recorded on 5/17. Steelhead smolt production estimates using SPIs (3,802 with 95% confidence intervals of 3,440-4,245) was similar to those using more standard screw trap methods

  18. Evaluation of Fish Movements, Migration Patterns, and Population Abundance with Streamwidth PIT Tag Interrogation Systems, Final Report 2002.

    Energy Technology Data Exchange (ETDEWEB)

    Zydlewski, Gayle; Winter, Christiane; McClanahan, Dee

    2003-02-01

    Two remote Streamwidth PIT tag Interrogation systems (SPIs) were operated continuously for over one year to test the feasibility of these systems for generating movement, migration, survival and smolt production estimates for salmonids. A total of 1,588 juvenile (< 100 mm FL) naturally produced salmonids (7 coho salmon, 482 cutthroat trout, and 1,099 steelhead) were PIT tagged above the upstream-most SPI (9 sites approximately 1 linear km each) in Fall 2001. Age at tagging for wild caught cutthroat and steelhead was 1 year. SPIs were operating before any PIT tagged fish were released in the creek. Over 390,000 detections were recorded from October 2001 to 31 July 2002. Efficiencies were site dependent, but overall detection efficiency for the creek was 97% with 95% confidence intervals of 91-100%. PIT tag detection efficiency ranged from 55-100% depending on the SPI and varied throughout the year with average efficiencies of 73% and 89%. SPI efficiency of PIT tag detection was not completely dependent on electronics noise levels or environmental conditions. Fish from all tagging locations were detected at the SPIs. Steelhead and cutthroat trout were primarily detected moving in the Spring (April-June) coincident with the anticipated smolt migration. Steelhead were also detected moving past SPIs at lower numbers in the Fall and Winter. Travel time between SPIs (downstream movement) was highly dependent on time of year. Travel time in the Spring was significantly faster (34.4 {+-} 7.0 hours) for all species than during any other time of year (763.1 {+-} 267.0 hours). Steelhead and cutthroat migrating in the Spring were the same age as those that did not migrate in the Spring. Peak of steelhead migration recorded at the two SPIs was 5/11 and 5/12 and the peak in the screw trap was recorded on 5/17. Steelhead smolt production estimates using SPIs (3,802 with 95% confidence intervals of 3,440 - 4,245) was similar to those using more standard screw trap methods

  19. Detection Efficiency of a ToF Spectrometer from Heavy-Ion Elastic Recoil Detection

    International Nuclear Information System (INIS)

    Barbara, E. de; Marti, G. V.; Capurro, O. A.; Fimiani, L.; Mingolla, M. G.; Negri, A. E.; Arazi, A.; Figueira, J. M.; Pacheco, A. J.; Martinez Heimann, D.; Carnelli, P. F. F.; Fernandez Niello, J. O.

    2010-01-01

    The detection efficiency of a time-of-flight system based on two micro-channel plates (MCP) time zero detectors plus a conventional silicon surface barrier detector was obtained from heavy ion elastic recoil measurements (this ToF spectrometer is mainly devoted to measurements of total fusion cross section of weakly bound projectiles on different mass-targets systems). In this work we have used beams of 7 Li, 16 O, 32 S and 35 Cl to study the mass region of interest for its application to measurements fusion cross sections in the 6,7 Li+ 27 Al systems at energies around and above the Coulomb barrier (0.8V B ≤E≤2.0V B ). As the efficiency of a ToF spectrometer is strongly dependent on the energy and mass of the detected particles, we have covered a wide range of the scattered particle energies with a high degree of accuracy at the lowest energies. The different experimental efficiency curves obtained in that way were compared with theoretical electronic stopping power curves on carbon foils and were applied.

  20. Detecting binary black holes with efficient and reliable templates

    International Nuclear Information System (INIS)

    Damour, T.; Iyer, B.R.; Sathyaprakash, B.S.

    2001-01-01

    Detecting binary black holes in interferometer data requires an accurate knowledge of the orbital phase evolution of the system. From the point of view of data analysis one also needs fast algorithms to compute the templates that will be employed in searching for black hole binaries. Recently, there has been progress on both these fronts: On one hand, re-summation techniques have made it possible to accelerate the convergence of poorly convergent asymptotic post-Newtonian series and derive waveforms beyond the conventional adiabatic approximation. We now have a waveform model that extends beyond the inspiral regime into the plunge phase followed by the quasi-normal mode ringing. On the other hand, explicit Fourier domain waveforms have been derived that make the generation of waveforms fast enough so as not to be a burden on the computational resources required in filtering the detector data. These new developments should make it possible to efficiently and reliably search for black hole binaries in data from first interferometers. (author)

  1. Effects of image processing on the detective quantum efficiency

    Energy Technology Data Exchange (ETDEWEB)

    Park, Hye-Suk; Kim, Hee-Joung; Cho, Hyo-Min; Lee, Chang-Lae; Lee, Seung-Wan; Choi, Yu-Na [Yonsei University, Wonju (Korea, Republic of)

    2010-02-15

    The evaluation of image quality is an important part of digital radiography. The modulation transfer function (MTF), the noise power spectrum (NPS), and the detective quantum efficiency (DQE) are widely accepted measurements of the digital radiographic system performance. However, as the methodologies for such characterization have not been standardized, it is difficult to compare directly reported the MTF, NPS, and DQE results. In this study, we evaluated the effect of an image processing algorithm for estimating the MTF, NPS, and DQE. The image performance parameters were evaluated using the international electro-technical commission (IEC 62220-1)-defined RQA5 radiographic techniques. Computed radiography (CR) posterior-anterior (PA) images of a hand for measuring the signal to noise ratio (SNR), the slit images for measuring the MTF, and the white images for measuring the NPS were obtained, and various multi-Scale image contrast amplification (MUSICA) factors were applied to each of the acquired images. All of the modifications of the images obtained by using image processing had a considerable influence on the evaluated image quality. In conclusion, the control parameters of image processing can be accounted for evaluating characterization of image quality in same way. The results of this study should serve as a baseline for based on evaluating imaging systems and their imaging characteristics by MTF, NPS, and DQE measurements.

  2. Effects of image processing on the detective quantum efficiency

    Science.gov (United States)

    Park, Hye-Suk; Kim, Hee-Joung; Cho, Hyo-Min; Lee, Chang-Lae; Lee, Seung-Wan; Choi, Yu-Na

    2010-04-01

    Digital radiography has gained popularity in many areas of clinical practice. This transition brings interest in advancing the methodologies for image quality characterization. However, as the methodologies for such characterizations have not been standardized, the results of these studies cannot be directly compared. The primary objective of this study was to standardize methodologies for image quality characterization. The secondary objective was to evaluate affected factors to Modulation transfer function (MTF), noise power spectrum (NPS), and detective quantum efficiency (DQE) according to image processing algorithm. Image performance parameters such as MTF, NPS, and DQE were evaluated using the international electro-technical commission (IEC 62220-1)-defined RQA5 radiographic techniques. Computed radiography (CR) images of hand posterior-anterior (PA) for measuring signal to noise ratio (SNR), slit image for measuring MTF, white image for measuring NPS were obtained and various Multi-Scale Image Contrast Amplification (MUSICA) parameters were applied to each of acquired images. In results, all of modified images were considerably influence on evaluating SNR, MTF, NPS, and DQE. Modified images by the post-processing had higher DQE than the MUSICA=0 image. This suggests that MUSICA values, as a post-processing, have an affect on the image when it is evaluating for image quality. In conclusion, the control parameters of image processing could be accounted for evaluating characterization of image quality in same way. The results of this study could be guided as a baseline to evaluate imaging systems and their imaging characteristics by measuring MTF, NPS, and DQE.

  3. Effects of image processing on the detective quantum efficiency

    International Nuclear Information System (INIS)

    Park, Hye-Suk; Kim, Hee-Joung; Cho, Hyo-Min; Lee, Chang-Lae; Lee, Seung-Wan; Choi, Yu-Na

    2010-01-01

    The evaluation of image quality is an important part of digital radiography. The modulation transfer function (MTF), the noise power spectrum (NPS), and the detective quantum efficiency (DQE) are widely accepted measurements of the digital radiographic system performance. However, as the methodologies for such characterization have not been standardized, it is difficult to compare directly reported the MTF, NPS, and DQE results. In this study, we evaluated the effect of an image processing algorithm for estimating the MTF, NPS, and DQE. The image performance parameters were evaluated using the international electro-technical commission (IEC 62220-1)-defined RQA5 radiographic techniques. Computed radiography (CR) posterior-anterior (PA) images of a hand for measuring the signal to noise ratio (SNR), the slit images for measuring the MTF, and the white images for measuring the NPS were obtained, and various multi-Scale image contrast amplification (MUSICA) factors were applied to each of the acquired images. All of the modifications of the images obtained by using image processing had a considerable influence on the evaluated image quality. In conclusion, the control parameters of image processing can be accounted for evaluating characterization of image quality in same way. The results of this study should serve as a baseline for based on evaluating imaging systems and their imaging characteristics by MTF, NPS, and DQE measurements.

  4. Inntags: small self-structured epitopes for innocuous protein tagging.

    Science.gov (United States)

    Georgieva, Maya V; Yahya, Galal; Codó, Laia; Ortiz, Raúl; Teixidó, Laura; Claros, José; Jara, Ricardo; Jara, Mònica; Iborra, Antoni; Gelpí, Josep Lluís; Gallego, Carme; Orozco, Modesto; Aldea, Martí

    2015-10-01

    Protein tagging is widely used in approaches ranging from affinity purification to fluorescence-based detection in live cells. However, an intrinsic limitation of tagging is that the native function of the protein may be compromised or even abolished by the presence of the tag. Here we describe and characterize a set of small, innocuous protein tags (inntags) that we anticipate will find application in a variety of biological techniques.

  5. WebTag: Web browsing into sensor tags over NFC.

    Science.gov (United States)

    Echevarria, Juan Jose; Ruiz-de-Garibay, Jonathan; Legarda, Jon; Alvarez, Maite; Ayerbe, Ana; Vazquez, Juan Ignacio

    2012-01-01

    Information and Communication Technologies (ICTs) continue to overcome many of the challenges related to wireless sensor monitoring, such as for example the design of smarter embedded processors, the improvement of the network architectures, the development of efficient communication protocols or the maximization of the life cycle autonomy. This work tries to improve the communication link of the data transmission in wireless sensor monitoring. The upstream communication link is usually based on standard IP technologies, but the downstream side is always masked with the proprietary protocols used for the wireless link (like ZigBee, Bluetooth, RFID, etc.). This work presents a novel solution (WebTag) for a direct IP based access to a sensor tag over the Near Field Communication (NFC) technology for secure applications. WebTag allows a direct web access to the sensor tag by means of a standard web browser, it reads the sensor data, configures the sampling rate and implements IP based security policies. It is, definitely, a new step towards the evolution of the Internet of Things paradigm.

  6. WebTag: Web Browsing into Sensor Tags over NFC

    Directory of Open Access Journals (Sweden)

    Juan Jose Echevarria

    2012-06-01

    Full Text Available Information and Communication Technologies (ICTs continue to overcome many of the challenges related to wireless sensor monitoring, such as for example the design of smarter embedded processors, the improvement of the network architectures, the development of efficient communication protocols or the maximization of the life cycle autonomy. This work tries to improve the communication link of the data transmission in wireless sensor monitoring. The upstream communication link is usually based on standard IP technologies, but the downstream side is always masked with the proprietary protocols used for the wireless link (like ZigBee, Bluetooth, RFID, etc.. This work presents a novel solution (WebTag for a direct IP based access to a sensor tag over the Near Field Communication (NFC technology for secure applications. WebTag allows a direct web access to the sensor tag by means of a standard web browser, it reads the sensor data, configures the sampling rate and implements IP based security policies. It is, definitely, a new step towards the evolution of the Internet of Things paradigm.

  7. Tag Based Audio Search Engine

    OpenAIRE

    Parameswaran Vellachu; Sunitha Abburu

    2012-01-01

    The volume of the music database is increasing day by day. Getting the required song as per the choice of the listener is a big challenge. Hence, it is really hard to manage this huge quantity, in terms of searching, filtering, through the music database. It is surprising to see that the audio and music industry still rely on very simplistic metadata to describe music files. However, while searching audio resource, an efficient "Tag Based Audio Search Engine" is necessary. The current researc...

  8. Modeling of detective quantum efficiency considering scatter-reduction devices

    Energy Technology Data Exchange (ETDEWEB)

    Park, Ji Woong; Kim, Dong Woon; Kim, Ho Kyung [Pusan National University, Busan (Korea, Republic of)

    2016-05-15

    The reduction of signal-to-noise ratio (SNR) cannot be restored and thus has become a severe issue in digital mammography.1 Therefore, antiscatter grids are typically used in mammography. Scatter-cleanup performance of various scatter-reduction devices, such as air gaps,2 linear (1D) or cellular (2D) grids,3, 4 and slot-scanning devices,5 has been extensively investigated by many research groups. In the present time, a digital mammography system with the slotscanning geometry is also commercially available.6 In this study, we theoretically investigate the effect of scattered photons on the detective quantum efficiency (DQE) performance of digital mammography detectors by using the cascaded-systems analysis (CSA) approach. We show a simple DQE formalism describing digital mammography detector systems equipped with scatter reduction devices by regarding the scattered photons as additive noise sources. The LFD increased with increasing PMMA thickness, and the amounts of LFD indicated the corresponding SF. The estimated SFs were 0.13, 0.21, and 0.29 for PMMA thicknesses of 10, 20, and 30 mm, respectively. While the solid line describing the measured MTF for PMMA with 0 mm was the result of least-squares of regression fit using Eq. (14), the other lines were simply resulted from the multiplication of the fit result (for PMMA with 0 mm) with the (1-SF) estimated from the LFDs in the measured MTFs. Spectral noise-power densities over the entire frequency range were not much changed with increasing scatter. On the other hand, the calculation results showed that the spectral noise-power densities increased with increasing scatter. This discrepancy may be explained by that the model developed in this study does not account for the changes in x-ray interaction parameters for varying spectral shapes due to beam hardening with increasing PMMA thicknesses.

  9. Development of a high-efficient transformation system of Bacillus pumilus strain DX01 to facilitate gene isolation via gfp-tagged insertional mutagenesis and visualize bacterial colonization of rice roots.

    Science.gov (United States)

    Shen, Xinqian; Chen, Yunpeng; Liu, Tong; Hu, Xiaolu; Gu, Zhenfang

    2013-09-01

    A Tn5 transposition vector, pMOD-tet-egfp, was constructed and used for the random insertional mutagenesis of Bacillus pumilus. Various parameters were investigated to increase the transformation efficiency B. pumilus DX01 via Tn5 transposition complexes (transposome): bacterial growth phase, type of electroporation buffer, electric field strength, and recovery medium. Transformation efficiency was up to 3 × 10(4) transformants/μg of DNA under the optimized electroporation conditions, and a total of 1,467 gfp-tagged transformants were obtained. Fluorescence-activated cell sorting analysis showed that all gfp-tagged bacterial cells expressed GFP, indicating that foreign DNA has been successfully integrated into the genome of B. pumilus and expressed. Finally, flanking DNA sequences were isolated from several transformants and colonization of rice roots by B. pumilus DX01 was also studied. The method developed here will be useful for creating an insertion mutant library of gram-positive bacteria, thus facilitating their molecular genetic and cytological studies.

  10. Tagging vs. Controlled Vocabulary

    DEFF Research Database (Denmark)

    Bogers, Toine; Petras, Vivien

    2015-01-01

    elements like core bibliographic data, controlled vocabulary terms, reviews, and tags to the retrieval performance. Our comparison is done using a test collection of over 2 million book records with information elements from Amazon, the British Library, the Library of Congress, and LibraryThing. We find...... that tags and controlled vocabulary terms do not actually outperform each other consistently, but seem to provide complementary contributions: some information needs are best addressed using controlled vocabulary terms whereas other are best addressed using tags....

  11. Tags on healthcare information websites

    DEFF Research Database (Denmark)

    Lykke, Marianne; Ådland, Marit Kristine

    2018-01-01

    This paper explores tags and tagging behaviour on health information websites using an empirical, user-oriented, exploratory case study. Taggers and editors were interviewed about tags and tagging, while taggers solved tasks that included applying tags to a website. This qualitative data...... articles, request information, and value article content. Some of these show that tags are not only not only topical descriptions, but communicative by intent. This result can potentially inform the design of tagging features....

  12. W/Top/Higgs-tagging in ATLAS

    CERN Document Server

    Norjoharuddeen, Nurfikri; The ATLAS collaboration

    2017-01-01

    We present updates of W, Top and Higgs tagging studies with the ATLAS detector. The performance of 2 variable taggers, HEPTopTagger and shower deconstruction are compared in Monte Carlo simulations. To asses the modelling of the taggers’ performance, the tagging efficiencies are measured, with the full 2015+2016 dataset, in semi-leptonic top quark pair events and the background rejections are measured in dijet and photon+jet topologies. Recent developments in subjet reconstruction techniques for high transverse momentum Higgs->bb tagging are also presented.

  13. A modified version of the Monte Carlo computer code for calculating neutron detection efficiencies

    International Nuclear Information System (INIS)

    Nakayama, K.; Pessoa, E.F.; Douglas, R.A.

    1980-12-01

    A calculation of neutron detection efficiencies has been performed for organic scintillators using the Monte Carlo Method. Effects which contribute to the detection efficiency have been incorporated in the calculations as thoroughly as possible. The reliability of the results is verified by comparison with the efficiency measurements available in the literature for neutrons in the energy range between 1 and 170 MeV with neutron detection thresholds between O.1 and 22.3 MeV. (Author) [pt

  14. W and top tagging scale factors

    CERN Document Server

    CMS Collaboration

    2017-01-01

    This note presents an improved determination of the efficiency and data/MC scale factors for the identification of hadronically decaying top quarks using the full 2016 CMS dataset. Also shown is an improved measurement of the W boson tagging performance using the full 2016 CMS dataset. Finally methods for the extraction of W tagging scale factors at high transverse momentum using fully merged top quarks are shown.

  15. Ultra Wide Band RFID Neutron Tags for Nuclear Materials Monitoring

    Energy Technology Data Exchange (ETDEWEB)

    Nekoogar, F; Dowla, F; Wang, T

    2010-01-27

    Recent advancements in the ultra-wide band Radio Frequency Identification (RFID) technology and solid state pillar type neutron detectors have enabled us to move forward in combining both technologies for advanced neutron monitoring. The LLNL RFID tag is totally passive and will operate indefinitely without the need for batteries. The tag is compact, can be directly mounted on metal, and has high performance in dense and cluttered environments. The LLNL coin-sized pillar solid state neutron detector has achieved a thermal neutron detection efficiency of 20% and neutron/gamma discrimination of 1E5. These performance values are comparable to a fieldable {sup 3}He based detector. In this paper we will discuss features about the two technologies and some potential applications for the advanced safeguarding of nuclear materials.

  16. Ultra Wide Band RFID Neutron Tags for Nuclear Materials Monitoring

    International Nuclear Information System (INIS)

    Nekoogar, F.; Dowla, F.; Wang, T.

    2010-01-01

    Recent advancements in the ultra-wide band Radio Frequency Identification (RFID) technology and solid state pillar type neutron detectors have enabled us to move forward in combining both technologies for advanced neutron monitoring. The LLNL RFID tag is totally passive and will operate indefinitely without the need for batteries. The tag is compact, can be directly mounted on metal, and has high performance in dense and cluttered environments. The LLNL coin-sized pillar solid state neutron detector has achieved a thermal neutron detection efficiency of 20% and neutron/gamma discrimination of 1E5. These performance values are comparable to a fieldable 3 He based detector. In this paper we will discuss features about the two technologies and some potential applications for the advanced safeguarding of nuclear materials.

  17. QUAD TAG MODULE

    International Nuclear Information System (INIS)

    Shiino, K.; Maruyama, K.

    1981-08-01

    Electronic circuits called QUAD TAG MODULE were developed and constructed, which were of exclusive use for signals from scintillation counter hodoscope of the photon tagging system. The circuit has functions of amplifiers, discriminators, and strobed coincidences in one NIM module. A description on functions and specifications of the module is given. (author)

  18. Comparative Performance of Acoustic-tagged and PIT-tagged Juvenile Salmonids

    Energy Technology Data Exchange (ETDEWEB)

    Hockersmith, Eric E.; Brown, Richard S.; Liedtke, Theresa L.

    2008-02-01

    Numerous research tools and technologies are currently being used to evaluate fish passage and survival to determine the impacts of the Federal Columbia River Power System (FCRPS) on endangered and threatened juvenile salmonids, including PIT tags, balloon tags, hydroacoustic evaluations, radio telemetry, and acoustic telemetry. Each has advantages and disadvantages, but options are restricted in some situations because of limited capabilities of a specific technology, lack of detection capability downstream, or availability of adequate numbers of fish. However, there remains concern about the comparative effects of the tag or the tagging procedure on fish performance. The recently developed Juvenile Salmonid Acoustic Telemetry System (JSATS) acoustic transmitter is the smallest active acoustic tag currently available. The goal of this study was to determine whether fish tagged with the JSATS acoustic-telemetry tag can provide unbiased estimates of passage behavior and survival within the performance life of the tag. We conducted both field and laboratory studies to assess tag effects. For the field evaluation we released a total of 996 acoustic-tagged fish in conjunction with 21,026 PIT-tagged fish into the tailrace of Lower Granite Dam on 6 and 13 May. Travel times between release and downstream dams were not significantly different for the majority of the reaches between acoustic-tagged and PIT-tagged fish. In addition to the field evaluation, a series of laboratory experiments were conducted to determine if growth and survival of juvenile Chinook salmon surgically implanted with acoustic transmitters is different than untagged or PIT tagged juvenile Chinook salmon. Only yearling fish with integrated and non-integrated transmitters experienced mortalities, and these were low (<4.5%). Mortality among sub-yearling control and PIT-tag treatments ranged up to 7.7% while integrated and non-integrated treatments had slightly higher rates (up to 8.3% and 7

  19. An efficient and fast detection algorithm for multimode FBG sensing

    DEFF Research Database (Denmark)

    Ganziy, Denis; Jespersen, O.; Rose, B.

    2015-01-01

    We propose a novel dynamic gate algorithm (DGA) for fast and accurate peak detection. The algorithm uses threshold determined detection window and Center of gravity algorithm with bias compensation. We analyze the wavelength fit resolution of the DGA for different values of signal to noise ratio ...

  20. PIT Tagging Anurans

    Science.gov (United States)

    McCreary, Brome

    2008-01-01

    The following video demonstrates a procedure to insert a passive integrated transponder (PIT) tag under the skin of an anuran (frog or toad) for research and monitoring purposes. Typically, a 12.5 mm tag (0.5 in.) is used to uniquely identify individual anurans as smal as 40 mm (1.6 in.) in length from snout to vent. Smaller tags are also available and allow smaller anurans to be tagged. The procedure does not differ for other sizes of tages or other sizes of anurans. Anyone using this procedure should ensure that the tag is small enough to fit easily behind the sacral hump of the anuran, as shown in this video.

  1. Magnetospheric particle detection efficiency of a conical telescope

    International Nuclear Information System (INIS)

    Miah, M.A.; Mitchell, J.W.; Wefel, J.P.

    1989-01-01

    A semianalytic program has been developed to map the pitch angles of magnetospheric particles onto a detector telescope acceptance cone. The telescope fractional efficiency is defined as the fraction of the pitch angle cone in common with the telescope cone multiplied by the fractional perpendicular component of the exposed detector area, and normalized by 2π. Calculations have been performed as a function of the satellite's location, orbital inclination and the zenith angle of the telescope axis, both in dipole and real geomagnetic field models. At the dipole equator, the peak efficiency occurs at 90 0 pitch angle. In the real geomagnetic field model, the average value of the pitch angle for maximum efficiency is ≅ 88 0 . The efficiency function depends strongly upon latitude and is independent of longitude in a dipole field, but depends on longitude in the real field model. In either field model, altitude, angle of tilt and orbital inclination have little effect upon efficiency. The efficiency function calculated at the dipole equator can be used at the minimum magnetic field equator with little error, but not for points away from the B min position. The results are applied to calculate the absolute flux of magnetospheric particles observed near the equator. (orig.)

  2. ATLAS boosted object tagging 2

    CERN Document Server

    Caudron, Julien; The ATLAS collaboration

    2015-01-01

    A detailed study into the optimal techniques for identifying boosted hadronically decaying W or Z bosons is presented. Various algorithms for reconstructing, grooming and tagging bosonic jets are compared for W bosons with a wide range of transverse momenta using 8 TeV data and 8 TeV and 13 TeV MC simulations. In addition, given that a hadronic jet has been identified as resulting from the hadronic decay of a W or Z, a technique is developed to discriminate between W and Z bosons. The modeling of the tagging variables used in this technique is studied using 8 TeV pp collision data and systematic uncertainties for the tagger efficiency and fake rates are evaluated.

  3. Measuring the b-tagging efficiency in ATLAS data at √(s)=8 TeV and √(s)=13 TeV using the prelT-method

    International Nuclear Information System (INIS)

    Burmeister, Ingo

    2017-01-01

    This thesis presents the first measurement of the b-tagging efficiency ε b with the p T rel -method under Run 2 conditions at the ATLAS experiment. The measurement has been performed for semi-leptonically decaying b-hadrons with a muon in the final state. This has been achieved by selecting events with a muon associated to a jet by a cone based matching algorithm. The p T rel -variable is sensitive to the mass of the weakly decaying particle and is therefore sensitive to the jet-flavor. Exploiting this dependence, the flavor fractions are determined with a log-likelihood template fit to the p T rel -distribution in the tagged and untagged jet samples determined by the algorithm and operating point under study. The dataset used for this study corresponds to an integrated luminosity of 11.6 fb -1 of proton-proton-collisions taken with the ATLAS detector at √(s)=13 TeV center of mass energy. In addition, ε b has been measured for algorithms on trigger level using the p T rel -method. This has been conducted in a set of proton-proton-collisions recorded at the ATLAS experiment at √(s)=8 TeV center of mass energy. Here, a conditional calibration depending on off-line tagger decisions has been utilized.

  4. DICOM involving XML path-tag

    Science.gov (United States)

    Zeng, Qiang; Yao, Zhihong; Liu, Lei

    2011-03-01

    Digital Imaging and Communications in Medicine (DICOM) is a standard for handling, storing, printing, and transmitting information in medical imaging. XML (Extensible Markup Language) is a set of rules for encoding documents in machine-readable form which has become more and more popular. The combination of these two is very necessary and promising. Using XML tags instead of numeric labels in DICOM files will effectively increase the readability and enhance the clear hierarchical structure of DICOM files. However, due to the fact that the XML tags rely heavily on the orders of the tags, the strong data dependency has a lot of influence on the flexibility of inserting and exchanging data. In order to improve the extensibility and sharing of DICOM files, this paper introduces XML Path-Tag to DICOM. When a DICOM file is converted to XML format, adding simple Path-Tag into the DICOM file in place of complex tags will keep the flexibility of a DICOM file while inserting data elements and give full play to the advantages of the structure and readability of an XML file. Our method can solve the weak readability problem of DICOM files and the tedious work of inserting data into an XML file. In addition, we set up a conversion engine that can transform among traditional DICOM files, XML-DCM and XML-DCM files involving XML Path-Tag efficiently.

  5. SCC: Semantic Context Cascade for Efficient Action Detection

    KAUST Repository

    Heilbron, Fabian Caba

    2017-11-09

    Despite the recent advances in large-scale video analysis, action detection remains as one of the most challenging unsolved problems in computer vision. This snag is in part due to the large volume of data that needs to be analyzed to detect actions in videos. Existing approaches have mitigated the computational cost, but still, these methods lack rich high-level semantics that helps them to localize the actions quickly. In this paper, we introduce a Semantic Cascade Context (SCC) model that aims to detect action in long video sequences. By embracing semantic priors associated with human activities, SCC produces high-quality class-specific action proposals and prune unrelated activities in a cascade fashion. Experimental results in ActivityNet unveils that SCC achieves state-of-the-art performance for action detection while operating at real time.

  6. Efficiently detecting outlying behavior in video-game players

    OpenAIRE

    Kim, Young Bin; Kang, Shin Jin; Lee, Sang Hyeok; Jung, Jang Young; Kam, Hyeong Ryeol; Lee, Jung; Kim, Young Sun; Lee, Joonsoo; Kim, Chang Hun

    2015-01-01

    In this paper, we propose a method for automatically detecting the times during which game players exhibit specific behavior, such as when players commonly show excitement, concentration, immersion, and surprise. The proposed method detects such outlying behavior based on the game players’ characteristics. These characteristics are captured non-invasively in a general game environment. In this paper, cameras were used to analyze observed data such as facial expressions and player movements. M...

  7. Efficient method for events detection in phonocardiographic signals

    Science.gov (United States)

    Martinez-Alajarin, Juan; Ruiz-Merino, Ramon

    2005-06-01

    The auscultation of the heart is still the first basic analysis tool used to evaluate the functional state of the heart, as well as the first indicator used to submit the patient to a cardiologist. In order to improve the diagnosis capabilities of auscultation, signal processing algorithms are currently being developed to assist the physician at primary care centers for adult and pediatric population. A basic task for the diagnosis from the phonocardiogram is to detect the events (main and additional sounds, murmurs and clicks) present in the cardiac cycle. This is usually made by applying a threshold and detecting the events that are bigger than the threshold. However, this method usually does not allow the detection of the main sounds when additional sounds and murmurs exist, or it may join several events into a unique one. In this paper we present a reliable method to detect the events present in the phonocardiogram, even in the presence of heart murmurs or additional sounds. The method detects relative maxima peaks in the amplitude envelope of the phonocardiogram, and computes a set of parameters associated with each event. Finally, a set of characteristics is extracted from each event to aid in the identification of the events. Besides, the morphology of the murmurs is also detected, which aids in the differentiation of different diseases that can occur in the same temporal localization. The algorithms have been applied to real normal heart sounds and murmurs, achieving satisfactory results.

  8. Image detection and compression for memory efficient system analysis

    Science.gov (United States)

    Bayraktar, Mustafa

    2015-02-01

    The advances in digital signal processing have been progressing towards efficient use of memory and processing. Both of these factors can be utilized efficiently by using feasible techniques of image storage by computing the minimum information of image which will enhance computation in later processes. Scale Invariant Feature Transform (SIFT) can be utilized to estimate and retrieve of an image. In computer vision, SIFT can be implemented to recognize the image by comparing its key features from SIFT saved key point descriptors. The main advantage of SIFT is that it doesn't only remove the redundant information from an image but also reduces the key points by matching their orientation and adding them together in different windows of image [1]. Another key property of this approach is that it works on highly contrasted images more efficiently because it`s design is based on collecting key points from the contrast shades of image.

  9. Stearic acids at sn-1, 3 positions of TAG are more efficient at limiting fat deposition than palmitic and oleic acids in C57BL/6 mice.

    Science.gov (United States)

    Gouk, Shiou-Wah; Cheng, Sit-Foon; Ong, Augustine Soon-Hock; Chuah, Cheng-Hock

    2014-04-14

    In the present study, we investigated the effect of long-acyl chain SFA, namely palmitic acid (16:0) and stearic acid (18:0), at sn-1, 3 positions of TAG on obesity. Throughout the 15 weeks of the experimental period, C57BL/6 mice were fed diets fortified with cocoa butter, sal stearin (SAL), palm mid fraction (PMF) and high-oleic sunflower oil (HOS). The sn-1, 3 positions were varied by 16:0, 18:0 and 18:1, whilst the sn-2 position was preserved with 18:1. The HOS-enriched diet was found to lead to the highest fat deposition. This was in accordance with our previous postulation. Upon normalisation of total fat deposited with food intake to obtain the fat:feed ratio, interestingly, mice fed the SAL-enriched diet exhibited significantly lower visceral fat/feed and total fat/feed compared with those fed the PMF-enriched diet, despite their similarity in SFA-unsaturated fatty acid-SFA profile. That long-chain SFA at sn-1, 3 positions concomitantly with an unsaturated FA at the sn-2 position exert an obesity-reducing effect was further validated. The present study is the first of its kind to demonstrate that SFA of different chain lengths at sn-1, 3 positions exert profound effects on fat accretion.

  10. Measurement of the neutron detection efficiency of a 80% absorber-20% scintillating fibers calorimeter

    Energy Technology Data Exchange (ETDEWEB)

    Anelli, M.; Bertolucci, S. [Laboratori Nazionali di Frascati dell' INFN, Via E.Fermi 40, I-00044 Frascati (Italy); Bini, C., E-mail: cesare.bini@roma1.infn.i [Dipartimento di Fisica, Sapienza Universita di Roma, P.le A.Moro, 2 I-00185 Roma (Italy); INFN Sezione di Roma, P.le A.Moro, 2 I-00185 Roma (Italy); Branchini, P. [INFN Sezione di Roma Tre, Via della Vasca Navale, 84 I-00146 Roma (Italy); Corradi, G.; Curceanu, C. [Laboratori Nazionali di Frascati dell' INFN, Via E.Fermi 40, I-00044 Frascati (Italy); De Zorzi, G.; Di Domenico, A. [Dipartimento di Fisica, Sapienza Universita di Roma, P.le A.Moro, 2 I-00185 Roma (Italy); INFN Sezione di Roma, P.le A.Moro, 2 I-00185 Roma (Italy); Di Micco, B. [Dipartimento di Fisica dell' Universita ' Roma Tre' , Via della Vasca Navale, 84 I-00146 Roma (Italy); INFN Sezione di Roma Tre, Via della Vasca Navale, 84 I-00146 Roma (Italy); Ferrari, A. [Institute of Safety Research and Institute of Radiation Physics, Forschungszentrum Dresden-Rossendorf, PF 510119, 01314 Dresden (Germany); Fiore, S. [Dipartimento di Fisica, Sapienza Universita di Roma, P.le A.Moro, 2 I-00185 Roma (Italy); INFN Sezione di Roma, P.le A.Moro, 2 I-00185 Roma (Italy); Gauzzi, P., E-mail: paolo.gauzzi@roma1.infn.i [Dipartimento di Fisica, Sapienza Universita di Roma, P.le A.Moro, 2 I-00185 Roma (Italy); INFN Sezione di Roma, P.le A.Moro, 2 I-00185 Roma (Italy); Giovannella, S.; Happacher, F. [Laboratori Nazionali di Frascati dell' INFN, Via E.Fermi 40, I-00044 Frascati (Italy); Iliescu, M. [Laboratori Nazionali di Frascati dell' INFN, Via E.Fermi 40, I-00044 Frascati (Italy); ' Horia Hulubei' National Institute of Physics and Nuclear Engineering, Str. Atomistilor no. 407, P.O. Box MG-6 Bucharest-Magurele (Romania); Luca, A.; Martini, M.; Miscetti, S. [Laboratori Nazionali di Frascati dell' INFN, Via E.Fermi 40, I-00044 Frascati (Italy)

    2011-01-21

    The neutron detection efficiency of a sampling calorimeter made of 1 mm diameter scintillating fibers embedded in a lead/bismuth structure has been measured at the neutron beam of The Svedberg Laboratory at Uppsala. A significant enhancement of the detection efficiency with respect to a bulk organic scintillator detector with the same thickness is observed.

  11. Comparison of genetic detection efficiency of different markers ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-06-03

    Jun 3, 2009 ... Chinese native sheep populations, Hu sheep, Tong sheep, Small-tailed Han sheep and Tan sheep were used to study the efficiency of genetic markers. The genetic markers used in this study include morphological and ecological indices, blood protein enzyme, microsatellite DNA and the combination of.

  12. A hybrid approach for efficient anomaly detection using metaheuristic methods.

    Science.gov (United States)

    Ghanem, Tamer F; Elkilani, Wail S; Abdul-Kader, Hatem M

    2015-07-01

    Network intrusion detection based on anomaly detection techniques has a significant role in protecting networks and systems against harmful activities. Different metaheuristic techniques have been used for anomaly detector generation. Yet, reported literature has not studied the use of the multi-start metaheuristic method for detector generation. This paper proposes a hybrid approach for anomaly detection in large scale datasets using detectors generated based on multi-start metaheuristic method and genetic algorithms. The proposed approach has taken some inspiration of negative selection-based detector generation. The evaluation of this approach is performed using NSL-KDD dataset which is a modified version of the widely used KDD CUP 99 dataset. The results show its effectiveness in generating a suitable number of detectors with an accuracy of 96.1% compared to other competitors of machine learning algorithms.

  13. A hybrid approach for efficient anomaly detection using metaheuristic methods

    Directory of Open Access Journals (Sweden)

    Tamer F. Ghanem

    2015-07-01

    Full Text Available Network intrusion detection based on anomaly detection techniques has a significant role in protecting networks and systems against harmful activities. Different metaheuristic techniques have been used for anomaly detector generation. Yet, reported literature has not studied the use of the multi-start metaheuristic method for detector generation. This paper proposes a hybrid approach for anomaly detection in large scale datasets using detectors generated based on multi-start metaheuristic method and genetic algorithms. The proposed approach has taken some inspiration of negative selection-based detector generation. The evaluation of this approach is performed using NSL-KDD dataset which is a modified version of the widely used KDD CUP 99 dataset. The results show its effectiveness in generating a suitable number of detectors with an accuracy of 96.1% compared to other competitors of machine learning algorithms.

  14. An Efficient Framework for Road Sign Detection and Recognition

    Directory of Open Access Journals (Sweden)

    Duanling Li

    2014-02-01

    Full Text Available Road sign detection and recognition is a significant and challenging issue not only for assisting drivers but also navigating mobile robots. In this paper, we propose a novel and fast approach for the automatic detection and recognition of road signs. First, we use Hue Saturation Intensity (HSI color space to segment the road signs color. And then we locate the road signs based on the geometry symmetry, as almost all the shapes of road sign shapes are symmetrical such circle, rectangle, triangle and octagon. The proposed shape feature is further applied to classify the shape initially. Finally, the road signs are exactly recognized by support vector machine (SVM classifiers. We test our proposed method on real road images and the experimental results show that it can detect and recognize road signs rapidly and accurately.

  15. Flavour Tagging at LHCb

    CERN Document Server

    Grabalosa Gandara, M

    2009-01-01

    To do precise CP violation measurements, the most possible accurate knowledge of the flavour at production of the reconstructed B meson is required. This poster summarizes the flavour tagging performances for the LHCb experiment. We use same side an opposite side algorithms to establish wheter the meson contained a b or a b\\bar quark. The final decision is obtained through a combination of several methods. The use of control channels, decays to a flavour specific final state, will allow to determine the wrong tag fraction \\omega (the probability of a tag to be wrong), which can be used as input for the determination of CKM unitary triangle angles.

  16. Efficient Indoor Proximity and Separation Detection for Location Fingerprinting

    DEFF Research Database (Denmark)

    Kjærgaard, Mikkel Baun; Treu, Georg; Ruppel, Peter

    2008-01-01

    , and for distance calculations the particular building topology has to be taken into account. To address these challenges, this paper presents a novel approach for indoor proximity and separation detection, which uses location fingerprinting for indoor positioning of targets and walking distances for modeling...

  17. An efficient visual saliency detection model based on Ripplet transform

    Indian Academy of Sciences (India)

    A Diana Andrushia

    Many of these computational models find application in adaptive image compression, segmentation of object-of-interest in an image, automatic image thumb- nailing, object detection and recognition, visual tracking, automatic creation of image collage, content-aware image resizing, non-photo realistic rendering, etc. [2].

  18. Efficient Incorporation of Markov Random Fields in Change Detection

    DEFF Research Database (Denmark)

    Aanæs, Henrik; Nielsen, Allan Aasbjerg; Carstensen, Jens Michael

    2009-01-01

    of noise, implying that the pixel-wise classifier is also noisy. There is thus a need for incorporating local homogeneity constraints into such a change detection framework. For this modelling task Markov Random Fields are suitable. Markov Random Fields have, however, previously been plagued by lack...

  19. Tag-to-Tag Interference Suppression Technique Based on Time Division for RFID

    Directory of Open Access Journals (Sweden)

    Grishma Khadka

    2017-01-01

    Full Text Available Radio-frequency identification (RFID is a tracking technology that enables immediate automatic object identification and rapid data sharing for a wide variety of modern applications using radio waves for data transmission from a tag to a reader. RFID is already well established in technical areas, and many companies have developed corresponding standards and measurement techniques. In the construction industry, effective monitoring of materials and equipment is an important task, and RFID helps to improve monitoring and controlling capabilities, in addition to enabling automation for construction projects. However, on construction sites, there are many tagged objects and multiple RFID tags that may interfere with each other’s communications. This reduces the reliability and efficiency of the RFID system. In this paper, we propose an anti-collision algorithm for communication between multiple tags and a reader. In order to suppress interference signals from multiple neighboring tags, the proposed algorithm employs the time-division (TD technique, where tags in the interrogation zone are assigned a specific time slot so that at every instance in time, a reader communicates with tags using the specific time slot. We present representative computer simulation examples to illustrate the performance of the proposed anti-collision technique for multiple RFID tags.

  20. Tagged Vector Contour (TVC)

    Data.gov (United States)

    Kansas Data Access and Support Center — The Kansas Tagged Vector Contour (TVC) dataset consists of digitized contours from the 7.5 minute topographic quadrangle maps. Coverage for the state is incomplete....

  1. Design of a passive UHF RFID tag for the ISO18000-6C protocol

    International Nuclear Information System (INIS)

    Wang Yao; Wen Guangjun; Mao Wei; He Yanli; Zhu Xueyong

    2011-01-01

    This paper presents a new fully integrated wide-range UHF passive RFID tag chip design that is compatible with the ISO18000-6C protocol. In order to reduce the die area, an ultra-low power CMOS voltage regulator without resistors and an area-efficient amplitude shift keying demodulator with a novel adaptive average generator are both adopted. A low power clock generator is designed to guarantee the accuracy of the clock under ±4%. As the clock gating technology is employed to reduce the power consumption of the baseband processor, the total power consumption of the tag is about 14 μW with a sensitivity of -9.5 dBm. The detection distance can reach about 5 m under 4 W effective isotropic radiated power. The whole tag is fabricated in TSMC 0.18 μm CMOS technology and the chip size is 880 x 880 μm 2 . (semiconductor integrated circuits)

  2. Charge Tags for Most Comprehensive ESI-MS Monitoring of Morita-Baylis-Hillman (MBH)/aza-MBH Reactions: Solid Mechanistic View and the Dualistic Role of the Charge Tagged Acrylate.

    Science.gov (United States)

    Galaverna, Renan; Camilo, Nilton S; Godoi, Marla N; Coelho, Fernando; Eberlin, Marcos N

    2016-02-05

    Neutral and charge tagged reagents were used to investigate the mechanism of the classical Morita-Baylis-Hillman (MBH) reaction as well as its aza-version using mass spectrometry with electrospray ionization (ESI-MS). The use of an acrylate (activated alkene) with a methylimidazolium ion as a charge tag eliminates the requirement for adding acids as ESI(+) additives, which are normally used to favor protonation and therefore detection of reaction partners (reagents, intermediates, and products) by ESI(+)-MS. For both charge tagged reactions (MBH/aza-MBH), most reactants, intermediates, and the final adducts were efficiently detected in the form of abundant doubly and singly charged ions. Characterization of the reactions partners was performed via both tandem mass spectrometry (ESI(+)-MS/MS) and accurate m/z measurements. The charge tagged reactions also showed faster conversion rates when compare to the neutral reaction, indicating a dualistic role for the charge tagged acrylate. It acts as both the reagent and a cocatalyst due to the inherent ionic-coordination nature of the methylimidazolium ion, which stabilizes the zwitterionic intermediates and reagents through different types of coordination ion pairs. Hemiacetal intermediates for the rate-limiting proton transfer step were also intercepted and characterized for both classical and aza-MBH charge tagged reactions.

  3. EFFICIENT LANE DETECTION BASED ON ARTIFICIAL NEURAL NETWORKS

    Directory of Open Access Journals (Sweden)

    F. Arce

    2017-09-01

    Full Text Available Lane detection is a problem that has attracted in the last years the attention of the computer vision community. Most of approaches used until now to face this problem combine conventional image processing, image analysis and pattern classification techniques. In this paper, we propose a methodology based on so-called Ellipsoidal Neural Networks with Dendritic Processing (ENNDPs as a new approach to provide a solution to this important problem. The functioning and performance of the proposed methodology is validated with a real video taken by a camera mounted on a car circulating on urban highway of Mexico City.

  4. Efficient Lane Detection Based on Artificial Neural Networks

    Science.gov (United States)

    Arce, F.; Zamora, E.; Hernández, G.; Sossa, H.

    2017-09-01

    Lane detection is a problem that has attracted in the last years the attention of the computer vision community. Most of approaches used until now to face this problem combine conventional image processing, image analysis and pattern classification techniques. In this paper, we propose a methodology based on so-called Ellipsoidal Neural Networks with Dendritic Processing (ENNDPs) as a new approach to provide a solution to this important problem. The functioning and performance of the proposed methodology is validated with a real video taken by a camera mounted on a car circulating on urban highway of Mexico City.

  5. The tagged photon beam polarization of the jet target experiment

    International Nuclear Information System (INIS)

    Bianchi, N.; Muccifora, V.

    1989-01-01

    The applicability of the residual electron selection method to the tagging method of the jet target laboratory has been studied. With this end in view the behaviour of the polarized bremsstrahlung cross section in the range considered has been analysed, while the polarization increase by means of the RES has been evaluated. The vertical conditions of the focusing of the tagging spectrometer as a function of energy have been determined. Finally the gamma beam density and the tagging efficiency have been calculated

  6. A cytochrome c fusion protein domain for convenient detection, quantification, and enhanced production of membrane proteins in Escherichia coli--expression and characterization of cytochrome-tagged Complex I subunits.

    Science.gov (United States)

    Gustavsson, Tobias; Trane, Maria; Moparthi, Vamsi K; Miklovyte, Egle; Moparthi, Lavanya; Górecki, Kamil; Leiding, Thom; Arsköld, Sindra Peterson; Hägerhäll, Cecilia

    2010-08-01

    Overproduction of membrane proteins can be a cumbersome task, particularly if high yields are desirable. NADH:quinone oxidoreductase (Complex I) contains several very large membrane-spanning protein subunits that hitherto have been impossible to express individually in any appreciable amounts in Escherichia coli. The polypeptides contain no prosthetic groups and are poorly antigenic, making optimization of protein production a challenging task. In this work, the C-terminal ends of the Complex I subunits NuoH, NuoL, NuoM, and NuoN from E. coli Complex I and the bona fide antiporters MrpA and MrpD were genetically fused to the cytochrome c domain of Bacillus subtilis cytochrome c(550). Compared with other available fusion-protein tagging systems, the cytochrome c has several advantages. The heme is covalently bound, renders the proteins visible by optical spectroscopy, and can be used to monitor, quantify, and determine the orientation of the polypeptides in a plethora of experiments. For the antiporter-like subunits NuoL, NuoM, and NuoN and the real antiporters MrpA and MrpD, unprecedented amounts of holo-cytochrome fusion proteins could be obtained in E. coli. The NuoHcyt polypeptide was also efficiently produced, but heme insertion was less effective in this construct. The cytochrome c(550) domain in all the fusion proteins exhibited normal spectra and redox properties, with an E(m) of about +170 mV. The MrpA and MrpD antiporters remained functional after being fused to the cytochrome c-tag. Finally, a his-tag could be added to the cytochrome domain, without any perturbations to the cytochrome properties, allowing efficient purification of the overexpressed fusion proteins.

  7. ATLAS Boosted Objects tagging performance

    CERN Document Server

    Ferreira de Lima, D E; The ATLAS collaboration

    2014-01-01

    In the session on the performance of jet substructure reconstruction and boosted object tagging. This first contribution focuses on boosted object tagging performance. Material includes the shower deconstruction CONF note (missed BOOST2013, public), the Boosted boson tagging note (ATL-PHYS-PUB-2014-004, public since April). New papers on W/Z dsicrimination or top tagging are unlikely.

  8. Facets: Ersatz, Resource and Tag

    Science.gov (United States)

    Frické, Martin H.

    2013-01-01

    Introduction: Faceted classification appears to be of utmost importance. Ersatz facets, resource faceting and tag faceting: The distinctions are drawn between facets and ersatz facets, and between faceted resources and faceted tags. Single tag resource faceting and multiple tag information object faceting: The basic features are explored of single…

  9. Photon-number-resolving SSPDs with system detection efficiency over 50% at telecom range

    Science.gov (United States)

    Zolotov, P.; Divochiy, A.; Vakhtomin, Yu.; Moshkova, M.; Morozov, P.; Seleznev, V.; Smirnov, K.

    2018-02-01

    We used technology of making high-efficiency superconducting single-photon detectors as a basis for improvement of photon-number-resolving devices. By adding optical cavity and using an improved NbN superconducting film, we enhanced previously reported system detection efficiency at telecom range for such detectors. Our results show that implementation of optical cavity helps to develop four-section device with quantum efficiency over 50% at 1.55 µm. Performed experimental studies of detecting multi-photon optical pulses showed irregularities over defining multi-photon through single-photon quantum efficiency.

  10. Group-based Motion Detection for Energy-Efficient Localisation

    Directory of Open Access Journals (Sweden)

    Alban Cotillon

    2012-10-01

    Full Text Available Long-term outdoor localization remains challenging due to the high energy profiles of GPS modules. Duty cycling the GPS module combined with inertial sensors can improve energy consumption. However, inertial sensors that are kept active all the time can also drain mobile node batteries. This paper proposes duty cycling strategies for inertial sensors to maintain a target position accuracy and node lifetime. We present a method for duty cycling motion sensors according to features of movement events, and evaluate its energy and accuracy profile for an empirical data trace of cattle movement. We further introduce the concept of group-based duty cycling, where nodes that cluster together can share the burden of motion detection to reduce their duty cycles. Our evaluation shows that both variants of motion sensor duty cycling yield up to 78% improvement in overall node power consumption, and that the group-based method yields an additional 20% power reduction during periods of low mobility.

  11. Development of techniques for tagging precursor and essential chemicals

    Energy Technology Data Exchange (ETDEWEB)

    Swansiger, W.A.; Shepodd, T.J. [Sandia National Labs., Livermore, CA (United States); Phillips, M.L.F. [Sandia National Labs., Albuquerque, NM (United States)

    1994-01-01

    The ability to identify the manufacturers and distributors of chemicals seized in raids of illicit drug labs would be of great value in controlling the diversion of these chemicals. We developed a tagging scheme based on the addition of sub-ppM concentrations of various combinations of rare-earth elements to the target chemicals and evaluated a number of techniques for detecting the tags. We developed soluble tags for tagging liquids and selected Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) as the preferred detection technique. We developed insoluble tags for tagging solids and developed methods to analyze them and mix them into solid precursors. We have successfully demonstrated the tagging of several solvents and two of the precursor chemicals used in one of the most popular clandestine methamphetamine syntheses (ephedrine reacting with hydriodic acid/red phosphorus). The tagging scheme is capable of yielding tens of thousands of signatures (using holmium as an internal standard and up to 9 rare-earths at up to 3 concentrations yields 3{sup 9} {minus} 1 = 19,682 signatures) and is applicable to most of the chemicals on the precursor and essential chemicals list. In the concentrations employed, the tags are safe enough to be added to pharmaceuticals and cheap enough to tag tanker loads of chemicals.

  12. Orientation-defined boundaries are detected with low efficiency.

    Science.gov (United States)

    Solomon, Joshua A; Morgan, Michael J

    2017-09-01

    When compared with other summary statistics (mean size, size variance, orientation variance), visual estimates of average orientation are inefficient. Observers act as if they use information from no more than two or three items. We hypothesised that observers would attain greater sampling efficiency when their task did not require an explicit representation of mean orientation. We tested this hypothesis using a texture-segmentation task. Two arrays of 32 wavelets each were presented; one left and one right of fixation. Orientations in the target array were sampled from wrapped normal distributions having two different means with the same variance. One distribution defined orientations above the horizontal meridian, the other defined orientations below the meridian. All orientations in the other array were defined by a single wrapped normal distribution having the same variance as each of the distributions in the target array. Contrary to our hypothesis, results indicate that observers effectively ignored all but one item from the top and bottom of each array. In fact, we found no change in the threshold difference between the target's two means when all but one item from the top and bottom of each array were removed. We are forced to conclude that the visual system does not compute the average of more than a few orientations, even for texture segmentation. Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. Patterned arrays for the efficient detection of whole cells

    Science.gov (United States)

    Alexander, Troy A.

    2006-10-01

    Surface-Enhanced-Raman-Spectroscopy (SERS) is potentially a very sensitive technique for the detection of biological agents (i.e., proteins, viruses or whole cell bacteria). However, since initial reports, its utility has not been realized. Its limited acceptance as a routine analysis technique for both chemical and biological agents is largely due to the lack of reproducible SERS-active substrates. Most established SERS substrate fabrication schemes are based on selfassembly of the metallic (typically, Au, Ag, Pt, Pd or Cu) surfaces responsible for enhancement. Further, these protocols do not lend themselves to the stringent control over the enhancing feature shape, size, and placement on a nanometer scale. SERS can be made a more robust and attractive spectroscopic technique for biological agents by developing quantifiable, highly sensitive, and highly selective SERS-active substrates. Recently, novel SERS-active substrates, fabricated from nano-patterned Si and Au have been commercialized and are easily obtained in the marketplace. Commercialized Au SERS-active substrates fabricated using semiconductor manipulation and routine metal vapor deposition techniques used for the spectral analysis of intact bacterial cells. This talk will focus on the substrate characterization (microscopic and spectral) and application towards whole cells.

  14. Theoretical determination of the neutron detection efficiency of plastic track detectors. Pt. 1

    International Nuclear Information System (INIS)

    Pretzsch, G.

    1982-01-01

    A theoretical model to determine the neutron detection efficiency of organic solid state nuclear track detectors without external radiator is described. The model involves the following calculation steps: production of heavy charged particles within the detector volume, characterization of the charged particles by appropriate physical quantities, application of suitable registration criteria, formation of etch pits. The etch pits formed are described by means of a distribution function which is doubly differential in both diameter and depth of the etch pits. The distribution function serves as the input value for the calculation of the detection efficiency. The detection efficiency is defined as the measured effect per neutron fluence. Hence it depends on the evaluation technique considered. The calculation of the distribution function is carried out for cellulose triacetate. The determination of the concrete detection efficiency using the light microscope and light transmission measurements as the evaluation technique will be described in further publications. (orig.)

  15. Entanglement of distant atoms by projective measurement: the role of detection efficiency

    Energy Technology Data Exchange (ETDEWEB)

    Zippilli, Stefano; Olivares-Renteria, Georgina A; Morigi, Giovanna [Departament de Fisica, Universitat Autonoma de Barcelona, E-08193 Bellaterra (Spain); Schuck, Carsten; Rohde, Felix; Eschner, Juergen [ICFO-Institut de Ciencies Fotoniques, E-08860 Castelldefels, Barcelona (Spain)], E-mail: stefano.zippilli@uab.es

    2008-10-15

    We assess proposals for entangling two distant atoms by measurement of emitted photons, analyzing how their performance depends on the photon detection efficiency. We consider schemes based on measurement of one or two photons and compare them in terms of the probability to obtain the detection event and of the conditional fidelity with which the desired entangled state is created. Based on an unraveling of the master equation, we quantify the parameter regimes in which one or the other scheme is more efficient, including the possible combination of the one-photon scheme with state purification. In general, protocols based on one-photon detection are more efficient in setups characterized by low photon detection efficiency, while at larger values two-photon protocols are preferable. We give numerical examples based on current experiments.

  16. Entanglement of distant atoms by projective measurement: the role of detection efficiency

    International Nuclear Information System (INIS)

    Zippilli, Stefano; Olivares-Renteria, Georgina A; Morigi, Giovanna; Schuck, Carsten; Rohde, Felix; Eschner, Juergen

    2008-01-01

    We assess proposals for entangling two distant atoms by measurement of emitted photons, analyzing how their performance depends on the photon detection efficiency. We consider schemes based on measurement of one or two photons and compare them in terms of the probability to obtain the detection event and of the conditional fidelity with which the desired entangled state is created. Based on an unraveling of the master equation, we quantify the parameter regimes in which one or the other scheme is more efficient, including the possible combination of the one-photon scheme with state purification. In general, protocols based on one-photon detection are more efficient in setups characterized by low photon detection efficiency, while at larger values two-photon protocols are preferable. We give numerical examples based on current experiments.

  17. Single-photon detectors combining high efficiency, high detection rates, and ultra-high timing resolution

    Directory of Open Access Journals (Sweden)

    Iman Esmaeil Zadeh

    2017-11-01

    Full Text Available Single-photon detection with high efficiency, high time resolution, low dark counts, and high photon detection rates is crucial for a wide range of optical measurements. Although efficient detectors have been reported before, combining all performance parameters in a single device remains a challenge. Here, we show a broadband NbTiN superconducting nanowire detector with an efficiency exceeding 92%, over 150 MHz photon detection rate, and a dark count rate below 130 Hz operated in a Gifford-McMahon cryostat. Furthermore, with careful optimization of the detector design and readout electronics, we reach an ultra-low system timing jitter of 14.80 ps (13.95 ps decoupled while maintaining high detection efficiencies (>75%.

  18. Development of T9 type thin gap chamber and measurement of the detection efficiency

    International Nuclear Information System (INIS)

    Yan Zhen; Feng Cunfeng; Zhu Chengguang; Sun Yansheng; Zhang Naijian; He Mao

    2006-01-01

    The development and production of 400 sets of T9 type TGC (Thin Gap Chamber) for ATLAS experiment are undertaken by Shandong University. The process of development of T9 type TGC is introduced first and then the method of measuring the detection efficiency of the TGC is described. The testing result of detection efficiency of 288 sets of T9 type TGC built by Shandong University shows that all the detectors excellently fulfill the quality requirements of ATLAS experiment. (authors)

  19. MR Colonography with fecal tagging: Barium vs. barium ferumoxsil

    DEFF Research Database (Denmark)

    Achiam, M.P.; Chabanova, E.; Logager, V.B.

    2008-01-01

    ) with fecal tagging may be a method of gaining further patient acceptance and widespread use, but the method has to be optimized. The aim of our study was to evaluate the quality of a new contrast agent mixture and to validate a new method for evaluating the tagging efficiency of contrast agents. Materials...

  20. Determination of local absolute detection efficiency of a ceratron with 55Fe Auger electrons

    International Nuclear Information System (INIS)

    Mori, C.; Sugiyama, T.; Watanabe, T.

    1983-01-01

    The local absolute detection efficiency of a Ceratron (channel electron multiplier made of ceramics) was determined with collimated Mn K Auger electrons ( 5 keV) emitted from 55 Fe as a function of electron incident position and applied voltage. The local efficiency at the channel inlet did not depend so much on the applied voltage. The efficiency at the funnel increased with the applied voltage, while it was always lower than that at the channel inlet. (orig.)

  1. An efficient and rapid method for protein detection with an example ...

    African Journals Online (AJOL)

    The CMW method has similar detection capabilities as the conventional CBB method. However, compared with the CBB protocol, it did not produce pungent odors, and was more efficient and rapid, which saved more than 4 to 10 h. This method is an acceptable alternative for the preliminary detection of protein expression.

  2. Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Vu Thi Ty Hang

    2017-09-01

    Full Text Available Background: Acute respiratory infections (ARI are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam. Methods: Four hundred and forty two nose and throat swabs were collected in viral transport medium, and were tested with Luminex xTAG Respiratory Viral Panel FAST v2. Multiplex RT-PCR and single RT-PCR were used as references.    Results: Overall, viral pathogens were detected in a total count of 270/294 (91.8%, 95% CI 88.1-94.7 by the Luminex among reference assays, whilst 112/6336 (1.8%, 95% CI, 1.4-2.1 of pathogens were detected by the Luminex, but not by reference assays. Frequency of pathogens detected by Luminex and reference assays was 379 and 292, respectively. The diagnostic yield was 66.7% (295/442, 95%CI 62.1-71.1% for the Luminex assay and 54.1% (239/442, 95% CI, 49.3-58.8% for reference assays. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range: 0.91 (0.83-1.00] were found for 10/15 viral agents. Conclusions: The Luminex assay is a high throughput multiplex platform for rapid detection of common viral pathogens causing ARI. Although the current high cost may prevent Luminex assays from being widely used, especially in limited resource settings where ARI are felt most, its introduction in clinical diagnostics may help reduce unnecessary use of antibiotic prescription.

  3. Tag gas burnup based on three-dimensional FTR analysis

    International Nuclear Information System (INIS)

    Kidman, R.B.

    1976-01-01

    Flux spectra from a three-dimensional diffusion theory analysis of the Fast Test Reactor (FTR) are used to predict gas tag ratio changes, as a function of exposure, for each FTR fuel and absorber subassembly plenum. These flux spectra are also used to predict Xe-125 equilibrium activities in absorber plena in order to assess the feasibility of using Xe-125 gamma rays to detect and distinguish control rod failures from fuel rod failures. Worst case tag burnup changes are used in conjunction with burnup and mass spectrometer uncertainties to establish the minimum spacing of tags which allows the tags to be unambiguously identified

  4. Evaluation of the efficiency of FDG PET/CT in detection and characterization of skeletal metastases

    Directory of Open Access Journals (Sweden)

    Ahmed Wafaie

    2014-03-01

    Conclusion: Fused PET/CT was highly efficient in evaluation of skeletal metastases with superior performance in: detection of early bone marrow infiltration not apparent on CT, resolution of metabolic activity before definite signs of complete healing on CT, detection of missed sclerotic metastases on PET due to their relatively low metabolic activity, detection of intra and extra osseous recurrence and differentiation of benign from malignant bone lesions.

  5. Tagging behaviour with support from controlled vocabulary

    DEFF Research Database (Denmark)

    Lykke, Marianne; Høj, Anne Lyhne; Madsen, Line Nørgaard

    2011-01-01

    The paper investigates how knowledge structures from a controlled vocabulary affect tagging. The study is a comparative analysis of tags assigned in two tagging systems, a simple tagging system (control system) that provides suggestions from two tag clouds (all users tags and my tags) and an enha...

  6. Low Power High-Efficiency Shift Register Using Implicit Pulse-Triggered Flip-Flop in 130 nm CMOS Process for a Cryptographic RFID Tag

    Directory of Open Access Journals (Sweden)

    Mohammad Torikul Islam Badal

    2016-12-01

    Full Text Available The shift register is a type of sequential logic circuit which is mostly used for storing digital data or the transferring of data in the form of binary numbers in radio frequency identification (RFID applications to improve the security of the system. A power-efficient shift register utilizing a new flip-flop with an implicit pulse-triggered structure is presented in this article. The proposed flip-flop has features of high performance and low power. It is composed of a sampling circuit implemented by five transistors, a C-element for rise and fall paths, and a keeper stage. The speed is enhanced by executing four clocked transistors together with a transition condition technique. The simulation result confirms that the proposed topology consumes the lowest amounts of power of 30.1997 and 22.7071 nW for parallel in –parallel out (PIPO and serial in –serial out (SISO shift register respectively covering 22 µm2 chip area. The overall design consist of only 16 transistors and is simulated in 130 nm complementary-metal-oxide-semiconductor (CMOS technology with a 1.2 V power supply.

  7. Efficient optimal joint channel estimation and data detection for massive MIMO systems

    KAUST Repository

    Alshamary, Haider Ali Jasim

    2016-08-15

    In this paper, we propose an efficient optimal joint channel estimation and data detection algorithm for massive MIMO wireless systems. Our algorithm is optimal in terms of the generalized likelihood ratio test (GLRT). For massive MIMO systems, we show that the expected complexity of our algorithm grows polynomially in the channel coherence time. Simulation results demonstrate significant performance gains of our algorithm compared with suboptimal non-coherent detection algorithms. To the best of our knowledge, this is the first algorithm which efficiently achieves GLRT-optimal non-coherent detections for massive MIMO systems with general constellations.

  8. The use of external electronic tags on fish: an evaluation of tag retention and tagging effects

    DEFF Research Database (Denmark)

    Jepsen, Niels; Thorstad, Eva B.; Havn, Torgeir

    2015-01-01

    publications, reporting effects of external tagging for 80 different fish species, which constitute the main basis for this review. External attachment holds certain benefits compared to other tagging methods, for example, speed of application, and it may be the only option for fishes with a body shape...... unsuitable for surgical implantation, or when using tags with sensors recording the external environment. The most commonly reported problems with external tags are tissue damage, premature tag loss, and decreased swimming capacity, but the effects are highly context dependent and species specific. Reduced...... growth and survival have also been recorded, but direct mortality caused by external tagging seems rare. Most of the studies reviewed evaluate tag retention, survival, and tissue reactions. There is a general need for more research on the effects of external tagging of fish with electronic tags...

  9. TagPad

    DEFF Research Database (Denmark)

    Bornoe, Nis; Barkhuus, Louise

    2013-01-01

    The area of cyberinfrastructures has looked extensively at research within the natural sciences, however, the social sciences have been largely overlooked in terms of novel data collection and analysis systems. We developed a probe tool, TagPad, to look at the process for social science data...

  10. Memory Efficient VLSI Implementation of Real-Time Motion Detection System Using FPGA Platform

    Directory of Open Access Journals (Sweden)

    Sanjay Singh

    2017-06-01

    Full Text Available Motion detection is the heart of a potentially complex automated video surveillance system, intended to be used as a standalone system. Therefore, in addition to being accurate and robust, a successful motion detection technique must also be economical in the use of computational resources on selected FPGA development platform. This is because many other complex algorithms of an automated video surveillance system also run on the same platform. Keeping this key requirement as main focus, a memory efficient VLSI architecture for real-time motion detection and its implementation on FPGA platform is presented in this paper. This is accomplished by proposing a new memory efficient motion detection scheme and designing its VLSI architecture. The complete real-time motion detection system using the proposed memory efficient architecture along with proper input/output interfaces is implemented on Xilinx ML510 (Virtex-5 FX130T FPGA development platform and is capable of operating at 154.55 MHz clock frequency. Memory requirement of the proposed architecture is reduced by 41% compared to the standard clustering based motion detection architecture. The new memory efficient system robustly and automatically detects motion in real-world scenarios (both for the static backgrounds and the pseudo-stationary backgrounds in real-time for standard PAL (720 × 576 size color video.

  11. Ontologies and tag-statistics

    International Nuclear Information System (INIS)

    Tibély, Gergely; Vicsek, Tamás; Pollner, Péter; Palla, Gergely

    2012-01-01

    Due to the increasing popularity of collaborative tagging systems, the research on tagged networks, hypergraphs, ontologies, folksonomies and other related concepts is becoming an important interdisciplinary area with great potential and relevance for practical applications. In most collaborative tagging systems the tagging by the users is completely ‘flat’, while in some cases they are allowed to define a shallow hierarchy for their own tags. However, usually no overall hierarchical organization of the tags is given, and one of the interesting challenges of this area is to provide an algorithm generating the ontology of the tags from the available data. In contrast, there are also other types of tagged networks available for research, where the tags are already organized into a directed acyclic graph (DAG), encapsulating the ‘is a sub-category of’ type of hierarchy between each other. In this paper, we study how this DAG affects the statistical distribution of tags on the nodes marked by the tags in various real networks. The motivation for this research was the fact that understanding the tagging based on a known hierarchy can help in revealing the hidden hierarchy of tags in collaborative tagging systems. We analyse the relation between the tag-frequency and the position of the tag in the DAG in two large sub-networks of the English Wikipedia and a protein-protein interaction network. We also study the tag co-occurrence statistics by introducing a two-dimensional (2D) tag-distance distribution preserving both the difference in the levels and the absolute distance in the DAG for the co-occurring pairs of tags. Our most interesting finding is that the local relevance of tags in the DAG (i.e. their rank or significance as characterized by, e.g., the length of the branches starting from them) is much more important than their global distance from the root. Furthermore, we also introduce a simple tagging model based on random walks on the DAG, capable of

  12. Ontologies and tag-statistics

    Science.gov (United States)

    Tibély, Gergely; Pollner, Péter; Vicsek, Tamás; Palla, Gergely

    2012-05-01

    Due to the increasing popularity of collaborative tagging systems, the research on tagged networks, hypergraphs, ontologies, folksonomies and other related concepts is becoming an important interdisciplinary area with great potential and relevance for practical applications. In most collaborative tagging systems the tagging by the users is completely ‘flat’, while in some cases they are allowed to define a shallow hierarchy for their own tags. However, usually no overall hierarchical organization of the tags is given, and one of the interesting challenges of this area is to provide an algorithm generating the ontology of the tags from the available data. In contrast, there are also other types of tagged networks available for research, where the tags are already organized into a directed acyclic graph (DAG), encapsulating the ‘is a sub-category of’ type of hierarchy between each other. In this paper, we study how this DAG affects the statistical distribution of tags on the nodes marked by the tags in various real networks. The motivation for this research was the fact that understanding the tagging based on a known hierarchy can help in revealing the hidden hierarchy of tags in collaborative tagging systems. We analyse the relation between the tag-frequency and the position of the tag in the DAG in two large sub-networks of the English Wikipedia and a protein-protein interaction network. We also study the tag co-occurrence statistics by introducing a two-dimensional (2D) tag-distance distribution preserving both the difference in the levels and the absolute distance in the DAG for the co-occurring pairs of tags. Our most interesting finding is that the local relevance of tags in the DAG (i.e. their rank or significance as characterized by, e.g., the length of the branches starting from them) is much more important than their global distance from the root. Furthermore, we also introduce a simple tagging model based on random walks on the DAG, capable of

  13. Associated Particle Tagging (APT) in Magnetic Spectrometers

    Energy Technology Data Exchange (ETDEWEB)

    Jordan, David V.; Baciak, James E.; Stave, Sean C.; Chichester, David; Dale, Daniel; Kim, Yujong; Harmon, Frank

    2012-10-16

    Summary In Brief The Associated Particle Tagging (APT) project, a collaboration of Pacific Northwest National Laboratory (PNNL), Idaho National Laboratory (INL) and the Idaho State University (ISU)/Idaho Accelerator Center (IAC), has completed an exploratory study to assess the role of magnetic spectrometers as the linchpin technology in next-generation tagged-neutron and tagged-photon active interrogation (AI). The computational study considered two principle concepts: (1) the application of a solenoidal alpha-particle spectrometer to a next-generation, large-emittance neutron generator for use in the associated particle imaging technique, and (2) the application of tagged photon beams to the detection of fissile material via active interrogation. In both cases, a magnetic spectrometer momentum-analyzes charged particles (in the neutron case, alpha particles accompanying neutron generation in the D-T reaction; in the tagged photon case, post-bremsstrahlung electrons) to define kinematic properties of the relevant neutral interrogation probe particle (i.e. neutron or photon). The main conclusions of the study can be briefly summarized as follows: Neutron generator: • For the solenoidal spectrometer concept, magnetic field strengths of order 1 Tesla or greater are required to keep the transverse size of the spectrometer smaller than 1 meter. The notional magnetic spectrometer design evaluated in this feasibility study uses a 5-T magnetic field and a borehole radius of 18 cm. • The design shows a potential for 4.5 Sr tagged neutron solid angle, a factor of 4.5 larger than achievable with current API neutron-generator designs. • The potential angular resolution for such a tagged neutron beam can be less than 0.5o for modest Si-detector position resolution (3 mm). Further improvement in angular resolution can be made by using Si-detectors with better position resolution. • The report documents several features of a notional generator design incorporating the

  14. An ultra-low-power area-efficient non-volatile memory in a 0.18 μm single-poly CMOS process for passive RFID tags

    International Nuclear Information System (INIS)

    Jia Xiaoyun; Feng Peng; Zhang Shengguang; Wu Nanjian; Zhao Baiqin; Liu Su

    2013-01-01

    This paper presents an ultra-low-power area-efficient non-volatile memory (NVM) in a 0.18 μm single-poly standard CMOS process for passive radio frequency identification (RFID) tags. In the memory cell, a novel low-power operation method is proposed to realize bi-directional Fowler—Nordheim tunneling during write operation. Furthermore, the cell is designed with PMOS transistors and coupling capacitors to minimize its area. In order to improve its reliability, the cell consists of double floating gates to store the data, and the 1 kbit NVM was implemented in a 0.18 μm single-poly standard CMOS process. The area of the memory cell and 1 kbit memory array is 96 μm 2 and 0.12 mm 2 , respectively. The measured results indicate that the program/erase voltage ranges from 5 to 6 V The power consumption of the read/write operation is 0.19 μW/0.69 μW at a read/write rate of (268 kb/s)/(3.0 kb/s). (semiconductor integrated circuits)

  15. Application of the EXtrapolated Efficiency Method (EXEM) to infer the gamma-cascade detection efficiency in the actinide region

    International Nuclear Information System (INIS)

    Ducasse, Q.; Jurado, B.; Mathieu, L.; Marini, P.; Morillon, B.; Aiche, M.; Tsekhanovich, I.

    2016-01-01

    The study of transfer-induced gamma-decay probabilities is very useful for understanding the surrogate-reaction method and, more generally, for constraining statistical-model calculations. One of the main difficulties in the measurement of gamma-decay probabilities is the determination of the gamma-cascade detection efficiency. In Boutoux et al. (2013) [10] we developed the EXtrapolated Efficiency Method (EXEM), a new method to measure this quantity. In this work, we have applied, for the first time, the EXEM to infer the gamma-cascade detection efficiency in the actinide region. In particular, we have considered the 238 U(d,p) 239 U and 238 U( 3 He,d) 239 Np reactions. We have performed Hauser–Feshbach calculations to interpret our results and to verify the hypothesis on which the EXEM is based. The determination of fission and gamma-decay probabilities of 239 Np below the neutron separation energy allowed us to validate the EXEM.

  16. Determination of alpha particle detection efficiency of an imaging plate (IP) detector

    International Nuclear Information System (INIS)

    Rahman, N.M; Iida, Takao; Yamazawa, Hiromi; Moriizumi, Jun

    2006-01-01

    In order to determine the detection efficiency of the imaging plate (IP) detector, the true radioactivity of the alpha particles, which sampled in the collection media, should be known. The true radioactivity could be accurately predicted with the help of the reference alpha spectrometer measurement. The detection efficiency calculated for the IP was estimated with the theoretical curve and the experimental data. It is assumed that the air sample contained the decay products of both 222 Rn and 220 Rn series, the most significant sources of alpha particles. The present study estimated the detection efficiency of the IP as 39.3% with an uncertainty of 2.9 that is well enough to confirm the future use of the IP as a radiation detector. Experimental materials and methods are described. (S.Y.)

  17. Energy-efficient area coverage for intruder detection in sensor networks

    CERN Document Server

    He, Shibo; Li, Junkun

    2014-01-01

    This Springer Brief presents recent research results on area coverage for intruder detection from an energy-efficient perspective. These results cover a variety of topics, including environmental surveillance and security monitoring. The authors also provide the background and range of applications for area coverage and elaborate on system models such as the formal definition of area coverage and sensing models. Several chapters focus on energy-efficient intruder detection and intruder trapping under the well-known binary sensing model, along with intruder trapping under the probabilistic sens

  18. Minimum detection efficiency for the loophole-free confirmation of quantum contextuality

    International Nuclear Information System (INIS)

    Xiang Yang; Hong Fang-Yu

    2013-01-01

    Klyachko—Can—Binicioğlu—Shumovsky (KCBS) inequality is a Bell-like inequality, the violation of which can be used to confirm the existence of quantum contextuality. However, the imperfection of detection efficiency may cause the so-called loophole in actual KCBS's experiments. We derive an alternative KCBS inequality to deal with the loophole in actual KCBS's experiments. We prove that if the experimental data violate this KCBS inequality, the loophole-free violation of the original KCBS inequality will occur. We show that the minimum detection efficiency needed for a loophole-free violation of the KCBS inequality is about 0.9738

  19. Minimum detection efficiencies for a loophole-free observable-asymmetric Bell-type test

    International Nuclear Information System (INIS)

    Garbarino, G.

    2010-01-01

    We discuss the problem of finding the most favorable conditions for closing the detection loophole in a test of local realism with a Bell inequality. For a generic nonmaximally entangled two-qubit state and two incompatible bases to be adopted for alternative measurements of two observables a and b on each party, we apply Hardy's proof of nonlocality without inequality and derive an Eberhard-like inequality. For an infinity of nonmaximally entangled states we find that it is possible to refute local realism by requiring perfect detection efficiency for only one of the two observables, say b, to be measured on each party: The test is free from the detection loophole for any value of the detection efficiency corresponding to the other observable a. The maximum tolerable noise in such a loophole-free observable-asymmetric test is also evaluated.

  20. Superior triacylglycerol (TAG) accumulation in starchless mutants of Scenedesmus obliquus: (II) evaluation of TAG yield and productivity in controlled photobioreactors

    Science.gov (United States)

    2014-01-01

    Background Many microalgae accumulate carbohydrates simultaneously with triacylglycerol (TAG) upon nitrogen starvation, and these products compete for photosynthetic products and metabolites from the central carbon metabolism. As shown for starchless mutants of the non-oleaginous model alga Chlamydomonas reinhardtii, reduced carbohydrate synthesis can enhance TAG production. However, these mutants still have a lower TAG productivity than wild-type oleaginous microalgae. Recently, several starchless mutants of the oleaginous microalga Scenedesmus obliquus were obtained which showed improved TAG content and productivity. Results The most promising mutant, slm1, is compared in detail to wild-type S. obliquus in controlled photobioreactors. In the slm1 mutant, the maximum TAG content increased to 57 ± 0.2% of dry weight versus 45 ± 1% in the wild type. In the wild type, TAG and starch were accumulated simultaneously during initial nitrogen starvation, and starch was subsequently degraded and likely converted into TAG. The starchless mutant did not produce starch and the liberated photosynthetic capacity was directed towards TAG synthesis. This increased the maximum yield of TAG on light by 51%, from 0.144 ± 0.004 in the wild type to 0.217 ± 0.011 g TAG/mol photon in the slm1 mutant. No differences in photosynthetic efficiency between the slm1 mutant and the wild type were observed, indicating that the mutation specifically altered carbon partitioning while leaving the photosynthetic capacity unaffected. Conclusions The yield of TAG on light can be improved by 51% by using the slm1 starchless mutant of S. obliquus, and a similar improvement seems realistic for the areal productivity in outdoor cultivation. The photosynthetic performance is not negatively affected in the slm1 and the main difference with the wild type is an improved carbon partitioning towards TAG. PMID:24883102

  1. Toward an Integrated Framwork for Data-Efficient Parametric Adaptive Detection

    Science.gov (United States)

    2012-02-27

    SINR and smallest MSE among all linear solutions in the Krylov subspace; and 2) is CFAR with non-decreasing detection probability as k increases...adaptive processing (STAP), reduced-rank detection, signal-to-interference-and-noise ratio (SINR), constant false-alarm rate ( CFAR ), efficient...covariance matrix of the disturbance (including interference, noise, etc.) and show that all CG-MF detectors attain constant false alarm rate ( CFAR ) and

  2. Marginal space learning for medical image analysis efficient detection and segmentation of anatomical structures

    CERN Document Server

    Zheng, Yefeng

    2014-01-01

    Presents an award winning image analysis technology (Thomas Edison Patent Award, MICCAI Young Investigator Award) that achieves object detection and segmentation with state-of-the-art accuracy and efficiency Flexible, machine learning-based framework, applicable across multiple anatomical structures and imaging modalities Thirty five clinical applications on detecting and segmenting anatomical structures such as heart chambers and valves, blood vessels, liver, kidney, prostate, lymph nodes, and sub-cortical brain structures, in CT, MRI, X-Ray and Ultrasound.

  3. An Efficient Implementation of FPGA Based Face Detection and Face Recognition System Using haar Classifiers

    OpenAIRE

    G,Premalatha

    2013-01-01

    This paper introduces a novel technique to detect faces similarly recognizes in real-time with very high rate. It is essentially a feature-based approach, in which a classifier is trained for Haar-like rectangular features selected by Ada Boost algorithm and efficient representation method histogram equalization is used for varying illumination in the image. The face detection system generates an integral image window to perform a Haar feature classification during one clock cycle. And then i...

  4. Tag-elese or The Language of Tags

    Directory of Open Access Journals (Sweden)

    Jan Simons

    2008-01-01

    Full Text Available The core "meme" of Web 2.0 from which almost all other memes radiated was: 'You control your own data' (O'Reilly, 2005, 3. Key instruments for this user control are tagging systems that allow users to freely assign keywords of their own choosing to Internet resources of their own making as well as to documents produced by others. Of course, freely chosen keywords tags do not necessarily follow prefixed taxonomies or classification systems. But going by the maxim that interaction creates similarity and similarity creates interaction, the idea - or hope - is, however, that the tagging practices of individual users will eventually converge into an emergent common vocabulary or folksonomy (Merholz, 2004; Shirky, 2005; Vander Wal, 2005b; Mika, 2007. It is far from clear, however, that free tagging systems will eventually yield controlled vocabularies, and there are many incentives for idiosyncratic, ambiguous, and inconsistent uses of tags. Left to themselves, free tagging systems seem to be too wild and too chaotic for any order to emerge. But are these free tagging systems really as "feral" as they seem to be, or do they only look uncontrolled because one has been looking for order in the wrong place? I have done a quick-and-dirty" analysis of Flickr's tag cloud. The concept was: if folksonomies encourage users to tap on their own vernacular, everyday natural language must somehow "guide" the tagging practices of users of tagging systems. Flickr's tag cloud has been choosen because it may teach us something about tagging systems and folksonomies, and not - or not primarily - because of what tags may tell us about pictures.

  5. Efficient drilling problem detection. Early fault detection by the combination of physical models and artificial intelligence

    Energy Technology Data Exchange (ETDEWEB)

    Nyboe, Roar

    2009-09-15

    The drilling of an oil or gas well is an expensive undertaking. Hence, it is not surprising that mistakes and accidents during drilling incur a high cost. Accidents could result in the loss of expensive equipment and subsequent delays setting back the operation for days or weeks and thus running up large bills on rig-time and personnel hours. Some types of accidents also pose a risk to the personnel or the environment. In this dissertation we study alarm systems which could give the driller an early warning of upcoming problems, and thus provide time to avoid these accidents. We explore alarm systems which combine advanced physical models of the well and drilling process with artificial intelligence and time series analysis. Finally, we determine the advantages as well as the challenges of this approach. It is our hope that this dissertation is accessible to both practitioners in machine learning and control engineering, as well as to petroleum engineers with a passing familiarity with machine learning. Hence this dissertation starts with a quick introduction to drilling problems and some terms from time series analysis and machine learning. We then briefly describe the theory of observer-based fault detection and isolation. Theories of supervisory control systems are also introduced, as these concern both the choice of algorithms and how AI-based alarm systems integrate with the rest of the operation. From chapter 6 and onward, the challenges to fault detection in drilling are discussed. We focus on clarifying what restrictions the available training data put on our choice of machine learning methods. In chapter 8 and 9, we propose ways to combine machine learning and observer-based fault detection. Experimental results are presented in chapter 10, before we end with concluding remarks in chapter 11. Our main conclusion, reflected in our experimental results, is that physical models and artificial intelligence can be combined to produce hybrid alarm systems that

  6. Energy-Efficient Fault-Tolerant Dynamic Event Region Detection in Wireless Sensor Networks

    DEFF Research Database (Denmark)

    Enemark, Hans-Jacob; Zhang, Yue; Dragoni, Nicola

    2015-01-01

    Fault-tolerant event detection is fundamental to wireless sensor network applications. Existing approaches usually adopt neighborhood collaboration for better detection accuracy, while need more energy consumption due to communication. Focusing on energy efficiency, this paper makes an improvement...... to a hybrid algorithm for dynamic event region detection, such as real-time tracking of chemical leakage regions. Considering the characteristics of the moving away dynamic events, we propose a return back condition for the hybrid algorithm from distributed neighborhood collaboration, in which a node makes...

  7. VirusDetect: An automated pipeline for efficient virus discovery using deep sequencing of small RNAs

    Science.gov (United States)

    Accurate detection of viruses in plants and animals is critical for agriculture production and human health. Deep sequencing and assembly of virus-derived siRNAs has proven to be a highly efficient approach for virus discovery. However, to date no computational tools specifically designed for both k...

  8. Measurement and simulation of neutron detection efficiency in lead-scintillating fiber calorimeters

    International Nuclear Information System (INIS)

    Anelli, M.; Bertolucci, S.; Bini, C.; Branchini, P.; Curceanu, C.; De Zorzi, G.; Di Domenico, A.; Di Micco, B.; Ferrari, A.; Fiore, S.; Gauzzi, P.; Giovannella, S.; Happacher, F.; Iliescu, M.; Martini, M.; Miscetti, S.; Nguyen, F.; Passeri, A.; Prokofiev, A.; Sciascia, B.

    2009-01-01

    The overall detection efficiency to neutrons of a small prototype of the KLOE lead-scintillating fiber calorimeter has been measured at the neutron beam facility of The Svedberg Laboratory, TSL, Uppsala, in the kinetic energy range [5-175] MeV. The measurement of the neutron detection efficiency of a NE110 scintillator provided a reference calibration. At the lowest trigger threshold, the overall calorimeter efficiency ranges from 30% to 50%. This value largely exceeds the estimated 8-15% expected if the response were proportional only to the scintillator equivalent thickness. A detailed simulation of the calorimeter and of the TSL beam line has been performed with the FLUKA Monte Carlo code. First data-MC comparisons are encouraging and allow to disentangle a neutron halo component in the beam.

  9. Measurement and simulation of neutron detection efficiency in lead-scintillating fiber calorimeters

    Energy Technology Data Exchange (ETDEWEB)

    Anelli, M.; Bertolucci, S. [Laboratori Nazionali di Frascati, INFN (Italy); Bini, C. [Dipartimento di Fisica dell' Universita ' La Sapienza' , Roma (Italy); INFN Sezione di Roma, Roma (Italy); Branchini, P. [INFN Sezione di Roma Tre, Roma (Italy); Curceanu, C. [Laboratori Nazionali di Frascati, INFN (Italy); De Zorzi, G.; Di Domenico, A. [Dipartimento di Fisica dell' Universita ' La Sapienza' , Roma (Italy); INFN Sezione di Roma, Roma (Italy); Di Micco, B. [Dipartimento di Fisica dell' Universita ' Roma Tre' , Roma (Italy); INFN Sezione di Roma Tre, Roma (Italy); Ferrari, A. [Fondazione CNAO, Milano (Italy); Fiore, S.; Gauzzi, P. [Dipartimento di Fisica dell' Universita ' La Sapienza' , Roma (Italy); INFN Sezione di Roma, Roma (Italy); Giovannella, S., E-mail: simona.giovannella@lnf.infn.i [Laboratori Nazionali di Frascati, INFN (Italy); Happacher, F. [Laboratori Nazionali di Frascati, INFN (Italy); Iliescu, M. [Laboratori Nazionali di Frascati, INFN (Italy); IFIN-HH, Bucharest (Romania); Martini, M. [Laboratori Nazionali di Frascati, INFN (Italy); Dipartimento di Energetica dell' Universita ' La Sapienza' , Roma (Italy); Miscetti, S. [Laboratori Nazionali di Frascati, INFN (Italy); Nguyen, F. [Dipartimento di Fisica dell' Universita ' Roma Tre' , Roma (Italy); INFN Sezione di Roma Tre, Roma (Italy); Passeri, A. [INFN Sezione di Roma Tre, Roma (Italy); Prokofiev, A. [Svedberg Laboratory, Uppsala University (Sweden); Sciascia, B. [Laboratori Nazionali di Frascati, INFN (Italy)

    2009-12-15

    The overall detection efficiency to neutrons of a small prototype of the KLOE lead-scintillating fiber calorimeter has been measured at the neutron beam facility of The Svedberg Laboratory, TSL, Uppsala, in the kinetic energy range [5-175] MeV. The measurement of the neutron detection efficiency of a NE110 scintillator provided a reference calibration. At the lowest trigger threshold, the overall calorimeter efficiency ranges from 30% to 50%. This value largely exceeds the estimated 8-15% expected if the response were proportional only to the scintillator equivalent thickness. A detailed simulation of the calorimeter and of the TSL beam line has been performed with the FLUKA Monte Carlo code. First data-MC comparisons are encouraging and allow to disentangle a neutron halo component in the beam.

  10. Social Tagging of Mission Data

    Science.gov (United States)

    Norris, Jeffrey S.; Wallick, Michael N.; Joswig, Joseph C.; Powell, Mark W.; Torres, Recaredo J.; Mittman, David S.; Abramyan, Lucy; Crockett, Thomas M.; Shams, Khawaja S.; Fox, Jason M.; hide

    2010-01-01

    Mars missions will generate a large amount of data in various forms, such as daily plans, images, and scientific information. Often, there is a semantic linkage between images that cannot be captured automatically. Software is needed that will provide a method for creating arbitrary tags for this mission data so that items with a similar tag can be related to each other. The tags should be visible and searchable for all users. A new routine was written to offer a new and more flexible search option over previous applications. This software allows users of the MSLICE program to apply any number of arbitrary tags to a piece of mission data through a MSLICE search interface. The application of tags creates relationships between data that did not previously exist. These tags can be easily removed and changed, and contain enough flexibility to be specifically configured for any mission. This gives users the ability to quickly recall or draw attention to particular pieces of mission data, for example: Give a semantic and meaningful description to mission data; for example, tag all images with a rock in them with the tag "rock." Rapidly recall specific and useful pieces of data; for example, tag a plan as"driving template." Call specific data to a user s attention; for example, tag a plan as "for:User." This software is part of the MSLICE release, which was written in Java. It will run on any current Windows, Macintosh, or Linux system.

  11. Efficient alpha particle detection by CR-39 applying 50 Hz-HV electrochemical etching method

    International Nuclear Information System (INIS)

    Sohrabi, M.; Soltani, Z.

    2016-01-01

    Alpha particles can be detected by CR-39 by applying either chemical etching (CE), electrochemical etching (ECE), or combined pre-etching and ECE usually through a multi-step HF-HV ECE process at temperatures much higher than room temperature. By applying pre-etching, characteristics responses of fast-neutron-induced recoil tracks in CR-39 by HF-HV ECE versus KOH normality (N) have shown two high-sensitivity peaks around 5–6 and 15–16 N and a large-diameter peak with a minimum sensitivity around 10–11 N at 25°C. On the other hand, 50 Hz-HV ECE method recently advanced in our laboratory detects alpha particles with high efficiency and broad registration energy range with small ECE tracks in polycarbonate (PC) detectors. By taking advantage of the CR-39 sensitivity to alpha particles, efficacy of 50 Hz-HV ECE method and CR-39 exotic responses under different KOH normalities, detection characteristics of 0.8 MeV alpha particle tracks were studied in 500 μm CR-39 for different fluences, ECE duration and KOH normality. Alpha registration efficiency increased as ECE duration increased to 90 ± 2% after 6–8 h beyond which plateaus are reached. Alpha track density versus fluence is linear up to 10 6  tracks cm −2 . The efficiency and mean track diameter versus alpha fluence up to 10 6  alphas cm −2 decrease as the fluence increases. Background track density and minimum detection limit are linear functions of ECE duration and increase as normality increases. The CR-39 processed for the first time in this study by 50 Hz-HV ECE method proved to provide a simple, efficient and practical alpha detection method at room temperature. - Highlights: • Alpha particles of 0.8 MeV were detected in CR-39 by 50 Hz-HV ECE method. • Efficiency/track diameter was studied vs fluence and time for 3 KOH normality. • Background track density and minimum detection limit vs duration were studied. • A new simple, efficient and low-cost alpha detection method

  12. Construction of Highly Efficient Resonance Energy Transfer Platform inside a Nanosphere for Ultrasensitive Electrochemiluminescence Detection.

    Science.gov (United States)

    Chen, Miao-Miao; Wang, Ying; Cheng, Shi-Bo; Wen, Wei; Zhang, Xiuhua; Wang, Shengfu; Huang, Wei-Hua

    2018-03-29

    Electrochemiluminescence (ECL) detection has attracted increasing attention as a promising analytical approach. A considerable number of studies showed that ECL intensity can be definitely improved by resonance energy transfer (RET), while the RET efficiency is strongly dependent on the distance between exited donors and acceptors. Herein we disclose for the first time a highly enhanced RET strategy to promote the energy transfer efficiency by co-encapsulating the donor ([Ru(bpy)3]2+)/acceptor (CdTe quantum dots, CdTe QDs) pairs into a silica nanosphere. Plenty of [Ru(bpy)3]2+ and CdTe QDs closed packed inside a single nanosphere greatly shortens the electron-transfer path and increases the RET probability, therefore significantly enhancing the luminous efficiency. Further combining with molecularly imprinting technique, we develop a novel ECL sensor for ultrasensitive and highly selective detection of target molecules. Proof of concept experiments showed that extremely low detection limits of sub-fg/mL (S/N=3) with broad linear ranges (fg/mL to ng/mL) could be obtained for detection of two kinds of mycotoxin (α-ergocryptine and ochratoxin A) that are recognized as potential health hazards at very low concentrations. This strategy combining enhanced RET system and molecularly imprinting technique, represents a versatile ECL platform toward low-cost, rapid, ultrasensitive and highly selective detection of target molecules in diverse applications.

  13. High efficiency processing for reduced amplitude zones detection in the HRECG signal

    Science.gov (United States)

    Dugarte, N.; Álvarez, A.; Balacco, J.; Mercado, G.; Gonzalez, A.; Dugarte, E.; Olivares, A.

    2016-04-01

    Summary - This article presents part of a more detailed research proposed in the medium to long term, with the intention of establishing a new philosophy of electrocardiogram surface analysis. This research aims to find indicators of cardiovascular disease in its early stage that may go unnoticed with conventional electrocardiography. This paper reports the development of a software processing which collect some existing techniques and incorporates novel methods for detection of reduced amplitude zones (RAZ) in high resolution electrocardiographic signal (HRECG).The algorithm consists of three stages, an efficient processing for QRS detection, averaging filter using correlation techniques and a step for RAZ detecting. Preliminary results show the efficiency of system and point to incorporation of techniques new using signal analysis with involving 12 leads.

  14. An Energy efficient application specific integrated circuit for electrocardiogram feature detection and its potential for ambulatory cardiovascular disease detection.

    Science.gov (United States)

    Jain, Sanjeev Kumar; Bhaumik, Basabi

    2016-03-01

    A novel algorithm based on forward search is developed for real-time electrocardiogram (ECG) signal processing and implemented in application specific integrated circuit (ASIC) for QRS complex related cardiovascular disease diagnosis. The authors have evaluated their algorithm using MIT-BIH database and achieve sensitivity of 99.86% and specificity of 99.93% for QRS complex peak detection. In this Letter, Physionet PTB diagnostic ECG database is used for QRS complex related disease detection. An ASIC for cardiovascular disease detection is fabricated using 130-nm CMOS high-speed process technology. The area of the ASIC is 0.5 mm(2). The power dissipation is 1.73 μW at the operating frequency of 1 kHz with a supply voltage of 0.6 V. The output from the ASIC is fed to their Android application that generates diagnostic report and can be sent to a cardiologist through email. Their ASIC result shows average failed detection rate of 0.16% for six leads data of 290 patients in PTB diagnostic ECG database. They also have implemented a low-leakage version of their ASIC. The ASIC dissipates only 45 pJ with a supply voltage of 0.9 V. Their proposed ASIC is most suitable for energy efficient telemetry cardiovascular disease detection system.

  15. Passive UHF RFID Tag with Multiple Sensing Capabilities

    Directory of Open Access Journals (Sweden)

    José Fernández-Salmerón

    2015-10-01

    Full Text Available This work presents the design, fabrication, and characterization of a printed radio frequency identification tag in the ultra-high frequency band with multiple sensing capabilities. This passive tag is directly screen printed on a cardboard box with the aim of monitoring the packaging conditions during the different stages of the supply chain. This tag includes a commercial force sensor and a printed opening detector. Hence, the force applied to the package can be measured as well as the opening of the box can be detected. The architecture presented is a passive single-chip RFID tag. An electronic switch has been implemented to be able to measure both sensor magnitudes in the same access without including a microcontroller or battery. Moreover, the chip used here integrates a temperature sensor and, therefore, this tag provides three different parameters in every reading.

  16. Study of mast cell count in skin tags

    Directory of Open Access Journals (Sweden)

    Zaher Hesham

    2007-01-01

    Full Text Available Background: Skin tags or acrochordons are common tumors of middle-aged and elderly subjects. They consist of loose fibrous tissue and occur mainly on the neck and major flexures as small, soft, pedunculated protrusions. Objectives: The aim was to compare the mast cells count in skin tags to adjacent normal skin in diabetic and nondiabetic participants in an attempt to elucidate the possible role of mast cells in the pathogenesis of skin tags. Participants and Methods: Thirty participants with skin tags were divided into group I (15 nondiabetic participants and group II (15 diabetic participants. Three biopsies were obtained from each participant: a large skin tag, a small skin tag and adjacent normal skin. Mast cell count from all the obtained sections was carried out, and the mast cell density was expressed as the average mast cell count/high power field (HPF. Results: A statistically significant increase in mast cells count in skin tags in comparison to normal skin was detected in group I and group II. There was no statistically significant difference between mast cell counts in skin tags of both the groups. Conclusion: Both the mast cell mediators and hyperinsulinemia are capable of inducing fibroblast proliferation and epidermal hyperplasia that are the main pathologic abnormalities seen in all types of skin tags. However, the presence of mast cells in all examined skin tags regardless of diabetes and obesity may point to the possible crucial role of mast cells in the etiogenesis of skin tags through its interaction with fibroblasts and keratinocytes.

  17. A method to measure prompt fission neutron spectrum using gamma multiplicity tagging

    Energy Technology Data Exchange (ETDEWEB)

    Blain, E., E-mail: blaine2@rpi.edu; Daskalakis, A.; Block, R.C.; Barry, D.; Danon, Y.

    2016-01-01

    In order to improve on current prompt fission neutron spectrum measurements, a gamma multiplicity tagging method was developed at the Rensselaer Polytechnic Institute Gearttner Linear Accelerator Center. This method involves using a coincidence requirement on an array of BaF{sub 2} gamma detectors to determine the timing of a fission event. This allows for much larger fission samples to be used due to the higher penetrability of gammas compared to fission fragments. Additionally, since the method relies on gammas as opposed to fission fragments, the effects of the low level discriminator, used in fission chambers to eliminate alpha events, are not seen. A {sup 252}Cf fission chamber was constructed in order to determine the viability of this method as well as the efficiency when compared to a fission chamber. The implemented multiple gamma tagging method was found to accurately reproduce the prompt fission neutron spectrum for the spontaneous fission of {sup 252}Cf and to detect 30% of fission events. - Highlights: • A method measuring prompt fission neutron spectrum using gamma rays was proposed. • The method tags fission as reliably as a fission chamber and with better timing. • The gamma tag method shows good agreement with existing {sup 252}Cf data and models. • This eliminates the need of a fission chamber and allows larger samples to be measured.

  18. Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay

    Directory of Open Access Journals (Sweden)

    Dabisch-Ruthe Mareike

    2012-07-01

    Full Text Available Abstract Background A broad spectrum of pathogens is causative for respiratory tract infections, but symptoms are mostly similar. Therefore, the identification of the causative viruses and bacteria is only feasible using multiplex PCR or several monoplex PCR tests in parallel. Methods The analytical sensitivity of three multiplex PCR assays, RespiFinder-19, RespiFinder-SMART-22 and xTAG-Respiratory-Virus-Panel-Fast-Assay (RVP, were compared to monoplex real-time PCR with quantified standardized control material. All assays include the most common respiratory pathogens. Results To compare the analytical sensitivity of the multiplex assays, samples were inoculated with 13 different quantified viruses in the range of 101 to 105 copies/ml. Concordant results were received for rhinovirus, whereas the RVP detected influenzavirus, RSV and hMPV more frequently in low concentrations. The RespiFinder-19 and the RespiFinder-SMART-22 showed a higher analytical sensitivity for adenoviruses and coronaviruses, whereas the RVP was incapable to detect adenovirus and coronavirus in concentrations of 104 copies/ml. The RespiFinder-19 and RespiFinder-SMART-22A did not detect influenzaviruses (104 copies/ml and RSV (103 copies/ml. The detection of all 13 viruses in one sample was only achieved using monoplex PCR. To analyze possible competitive amplification reactions between the different viruses, samples were further inoculated with only 4 different viruses in one sample. Compared to the detection of 13 viruses in parallel, only a few differences were found. The incidence of respiratory viruses was compared in tracheal secretion (TS samples (n = 100 of mechanically ventilated patients in winter (n = 50 and summer (n = 50. In winter, respiratory viruses were detected in 32 TS samples (64% by RespiFinder-19, whereas the detection rate with RVP was only 22%. The most frequent viruses were adenovirus (32% and PIV-2 (20%. Multiple infections were detected

  19. Nuclear structure via isomer tagging of fission fragments

    Science.gov (United States)

    Wu, C. Y.; Cline, D.; Simon, M. W.; Stoyer, M. A.

    1997-10-01

    The high efficiency for detecting high-fold γ rays by large Ge arrays makes it possible to study the detailed spectroscopy of many neutron-rich nuclei produced by fission. Major progress has been made using sealed spontaneous fission sources. Considerable improvement in selectivity is provided, with an open source, both by gating on isomers and by detection of both fission fragments in coincidence with the deexcitation γ rays (see the preceding contribution). The reconstructed kinematics allows a measure of fragment mass and the Doppler shift correction of γ rays. In a recent experiment, fission fragments were detected using half of the CHICO array and an annular PPAC in coincidence with deexcitation γ rays detected by the Rochester array of eight Compton-suppressed Ge detectors. The annular PPAC was located only 1.0" from a 3.7 μCi ^252Cf source for efficient isomer tagging. The correlation was studied between delayed, within a time window between 150 ns and 10 μs after a fission occurring, and prompt γ rays. Several prominent feeding patterns to isomers in the mass region around 100 and 130 are identified by such correlation study. Experimental details and results will be presented.

  20. Review on SAW RFID tags.

    Science.gov (United States)

    Plessky, Victor P; Reindl, Leonhard M

    2010-03-01

    SAW tags were invented more than 30 years ago, but only today are the conditions united for mass application of this technology. The devices in the 2.4-GHz ISM band can be routinely produced with optical lithography, high-resolution radar systems can be built up using highly sophisticated, but low-cost RF-chips, and the Internet is available for global access to the tag databases. The "Internet of Things," or I-o-T, will demand trillions of cheap tags and sensors. The SAW tags can overcome semiconductor-based analogs in many aspects: they can be read at a distance of a few meters with readers radiating power levels 2 to 3 orders lower, they are cheap, and they can operate in robust environments. Passive SAW tags are easily combined with sensors. Even the "anti-collision" problem (i.e., the simultaneous reading of many nearby tags) has adequate solutions for many practical applications. In this paper, we discuss the state-of-the-art in the development of SAW tags. The design approaches will be reviewed and optimal tag designs, as well as encoding methods, will be demonstrated. We discuss ways to reduce the size and cost of these devices. A few practical examples of tags using a time-position coding with 10(6) different codes will be demonstrated. Phase-coded devices can additionally increase the number of codes at the expense of a reduction of reading distance. We also discuss new and exciting perspectives of using ultra wide band (UWB) technology for SAW-tag systems. The wide frequency band available for this standard provides a great opportunity for SAW tags to be radically reduced in size to about 1 x 1 mm(2) while keeping a practically infinite number of possible different codes. Finally, the reader technology will be discussed, as well as detailed comparison made between SAW tags and IC-based semiconductor device.

  1. Measurement and simulation of the neutron detection efficiency with a Pb-scintillating fiber calorimeter

    Energy Technology Data Exchange (ETDEWEB)

    Anelli, M; Bertolucci, S; Curceanu, C; Giovannella, S; Happacher, F; Iliescu, M; Martini, M; Miscetti, S [Laboratori Nazionali di Frascati, INFN (Italy); Battistoni, G [Sezione INFN di Milano (Italy); Bini, C; Zorzi, G De; Domenico, Adi; Gauzzi, P [Ubiversita degli Studi ' La Sapienza' e Sezine INFN di Roma (Italy); Branchini, P; Micco, B Di; Ngugen, F; Paseri, A [Universita degli di Studi ' Roma Tre' e Sezione INFN di Roma Tre (Italy); Ferrari, A [Fondazione CNAO, Milano (Italy); Prokfiev, A [Svedberg Laboratory, Uppsala University (Sweden); Fiore, S, E-mail: matteo.martino@inf.infn.i

    2009-04-01

    We have measured the overall detection efficiency of a small prototype of the KLOE PB-scintillation fiber calorimeter to neutrons with kinetic energy range [5,175] MeV. The measurement has been done in a dedicated test beam in the neutron beam facility of the Svedberg Laboratory, TSL Uppsala. The measurements of the neutron detection efficiency of a NE110 scintillator provided a reference calibration. At the lowest trigger threshold, the overall calorimeter efficiency ranges from 28% to 33%. This value largely exceeds the estimated {approx}8% expected if the response were proportional only to the scintillator equivalent thickness. A detailed simulation of the calorimeter and of the TSL beam line has been performed with the FLUKA Monte Carlo code. The simulated response of the detector to neutrons is presented together with the first data to Monte Carlo comparison. The results show an overall neutron efficiency of about 35%. The reasons for such an efficiency enhancement, in comparison with the typical scintillator-based neutron counters, are explained, opening the road to a novel neutron detector.

  2. Buddy Tag CONOPS and Requirements.

    Energy Technology Data Exchange (ETDEWEB)

    Brotz, Jay Kristoffer [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Deland, Sharon M. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-12-01

    This document defines the concept of operations (CONOPS) and the requirements for the Buddy Tag, which is conceived and designed in collaboration between Sandia National Laboratories and Princeton University under the Department of State Key VerificationAssets Fund. The CONOPS describe how the tags are used to support verification of treaty limitations and is only defined to the extent necessary to support a tag design. The requirements define the necessary functions and desired non-functional features of the Buddy Tag at a high level

  3. Flavour tagging performance in LHCb

    International Nuclear Information System (INIS)

    Grabalosa Gandara, Marc

    2009-01-01

    To do precise CP violation measurements, the best possible determination of the flavour of the B-meson is necessary. This report summarizes the flavour tagging performances for the LHCb experiment. The flavour tagging is obtained through a combination of several methods, based on different signatures. The use of control channels, which are decays to flavour-specific final states, will allow to determine the wrong tag fraction ω (the probability of a tag to be wrong), which can be used as an input for the determination of CKM unitarity triangle angles.

  4. Airborne virus sampling: Efficiencies of samplers and their detection limits for infectious bursal disease virus (IBDV

    Directory of Open Access Journals (Sweden)

    Yang Zhao

    2014-09-01

    Full Text Available [b]Introduction[/b]. The airborne transmission of infectious diseases in livestock production is increasingly receiving research attention. Reliable techniques of air sampling are crucial to underpin the findings of such studies. This study evaluated the physical and biological efficiencies and detection limits of four samplers (Andersen 6-stage impactor, all-glass impinger “AGI-30”, OMNI-3000 and MD8 with gelatin filter for collecting aerosols of infectious bursal disease virus (IBDV. [b]Materials and Method[/b]. IBDV aerosols mixed with a physical tracer (uranine were generated in an isolator, and then collected by the bioaerosol samplers. Samplers’ physical and biological efficiencies were derived based on the tracer concentration and the virus/tracer ratio, respectively. Detection limits for the samplers were estimated with the obtained efficiency data. [b]Results.[/b] Physical efficiencies of the AGI-30 (96% and the MD8 (100% were significantly higher than that of the OMNI-3000 (60%. Biological efficiency of the OMNI-3000 (23% was significantly lower than 100% (P < 0.01, indicating inactivation of airborne virus during sampling. The AGI-30, the Andersen impactor and the MD8 did not significantly inactivate virus during sampling. The 2-min detection limits of the samplers on airborne IBDV were 4.1 log[sub]10[/sub] 50% egg infective dose (EID[sub]50[/sub] m [sup]-3[/sup] for the Andersen impactor, 3.3 log[sub]10[/sub] EID50 m [sup]-3[/sup] for the AGI-30, 2.5 log[sub]10[/sub] EID50 m [sup]-3[/sup] for the OMNI-3000, and 2.9 log[sub]10[/sub] EID[sub]50[/sub] m [sup]-3[/sup] for the MD8. The mean half-life of IBDV aerosolized at 20 °C and 70% was 11.9 min. Conclusion. Efficiencies of different samplers vary. Despite its relatively low sampling efficiency, the OMNI-3000 is suitable for use in environments with low viral concentrations because its high flow rate gives a low detection limit. With the 4 samplers investigated, negative air

  5. Efficiency of Airborne Sample Analysis Platform (ASAP) bioaerosol sampler for pathogen detection.

    Science.gov (United States)

    Sharma, Anurag; Clark, Elizabeth; McGlothlin, James D; Mittal, Suresh K

    2015-01-01

    The threat of bioterrorism and pandemics has highlighted the urgency for rapid and reliable bioaerosol detection in different environments. Safeguarding against such threats requires continuous sampling of the ambient air for pathogen detection. In this study we investigated the efficacy of the Airborne Sample Analysis Platform (ASAP) 2800 bioaerosol sampler to collect representative samples of air and identify specific viruses suspended as bioaerosols. To test this concept, we aerosolized an innocuous replication-defective bovine adenovirus serotype 3 (BAdV3) in a controlled laboratory environment. The ASAP efficiently trapped the surrogate virus at 5 × 10(3) plaque-forming units (p.f.u.) [2 × 10(5) genome copy equivalent] concentrations or more resulting in the successful detection of the virus using quantitative PCR. These results support the further development of ASAP for bioaerosol pathogen detection.

  6. Efficient video-equipped fire detection approach for automatic fire alarm systems

    Science.gov (United States)

    Kang, Myeongsu; Tung, Truong Xuan; Kim, Jong-Myon

    2013-01-01

    This paper proposes an efficient four-stage approach that automatically detects fire using video capabilities. In the first stage, an approximate median method is used to detect video frame regions involving motion. In the second stage, a fuzzy c-means-based clustering algorithm is employed to extract candidate regions of fire from all of the movement-containing regions. In the third stage, a gray level co-occurrence matrix is used to extract texture parameters by tracking red-colored objects in the candidate regions. These texture features are, subsequently, used as inputs of a back-propagation neural network to distinguish between fire and nonfire. Experimental results indicate that the proposed four-stage approach outperforms other fire detection algorithms in terms of consistently increasing the accuracy of fire detection in both indoor and outdoor test videos.

  7. Efficiency of Airborne Sample Analysis Platform (ASAP Bioaerosol Sampler for Pathogen Detection

    Directory of Open Access Journals (Sweden)

    Anurag eSharma

    2015-05-01

    Full Text Available The threat of bioterrorism and pandemics has highlighted the urgency for rapid and reliable bioaerosol detection in different environments. Safeguarding against such threats requires continuous sampling of the ambient air for pathogen detection. In this study we investigated the efficacy of the Airborne Sample Analysis Platform (ASAP 2800 bioaerosol sampler to collect representative samples of air and identify specific viruses suspended as bioaerosols. To test this concept, we aerosolized an innocuous replication-defective bovine adenovirus serotype 3 (BAdV3 in a controlled laboratory environment. The ASAP efficiently trapped the surrogate virus at 5×10E3 plaque-forming units (p.f.u. [2×10E5 genome copy equivalent] concentrations or more resulting in the successful detection of the virus using quantitative PCR. These results support the further development of ASAP for bioaerosol pathogen detection.

  8. W-tagging performance in 13 TeV simulation

    CERN Document Server

    CMS Collaboration

    2016-01-01

    In the new energy regime explored by the Large Hadron Collider, it has become important to distinguish between jets originating from a single quark/gluon ("one-prong") and jets originating from the merging of the decay products of a W boson ("two-prong"). Several different algorithms have been developed to identify such merged W jets, with the same techniques also applicable for tagging highly energetic Z and Higgs bosons. In this note, the mistagging rate and efficiency for tagging W jets is measured in 13 TeV simulation for three different W-tagging algorithms.

  9. A measurement of Rb using mutually exclusive tags

    Science.gov (United States)

    Barate, R.; Buskulic, D.; Decamp, D.; Ghez, P.; Goy, C.; Lees, J.-P.; Lucotte, A.; Minard, M.-N.; Nief, J.-Y.; Pietrzyk, B.; Casado, M. P.; Chmeissani, M.; Comas, P.; Crespo, J. M.; Delfino, M.; Fernandez, E.; Fernandez-Bosman, M.; Garrido, Ll.; Juste, A.; Martinez, M.; Miquel, R.; Mir, Ll. M.; Orteu, S.; Padilla, C.; Park, I. C.; Pascual, A.; Perlas, J. A.; Riu, I.; Sanchez, F.; Teubert, F.; Colaleo, A.; Creanza, D.; de Palma, M.; Gelao, G.; Iaselli, G.; Maggi, G.; Maggi, M.; Marinelli, N.; Nuzzo, S.; Ranieri, A.; Raso, G.; Ruggieri, F.; Selvaggi, G.; Silvestris, L.; Tempesta, P.; Tricomi, A.; Zito, G.; Huang, X.; Lin, J.; Ouyang, Q.; Wang, T.; Xie, Y.; Xu, R.; Xue, S.; Zhang, J.; Zhang, L.; Zhao, W.; Abbaneo, D.; Alemany, R.; Becker, U.; Bazarko, A. O.; Bright-Thomas, P.; Cattaneo, M.; Cerutti, F.; Drevermann, H.; Forty, R. W.; Frank, M.; Hagelberg, R.; Harvey, J.; Janot, P.; Jost, B.; Kneringer, E.; Knobloch, J.; Lehraus, I.; Lutters, G.; Mato, P.; Minten, A.; Moneta, L.; Pacheco, A.; Pusztaszeri, J.-F.; Ranjard, F.; Rensing, P.; Rizzo, G.; Rolandi, L.; Schlatter, D.; Schmitt, M.; Schneider, O.; Tejessy, W.; Tomalin, I. R.; Wachsmuth, H.; Wagner, A.; Ajaltouni, Z.; Barrès, A.; Boyer, C.; Falvard, A.; Ferdi, C.; Gay, P.; Guicheney, C.; Henrard, P.; Jousset, J.; Michel, B.; Monteil, S.; Montret, J.-C.; Pallin, D.; Perret, P.; Podlyski, F.; Proriol, J.; Rosnet, P.; Rossignol, J.-M.; Fearnley, T.; Hansen, J. B.; Hansen, J. D.; Hansen, J. R.; Hansen, P. H.; Nilsson, B. S.; Rensch, B.; Wäänänen, A.; Daskalakis, G.; Kyriakis, A.; Markou, C.; Simopoulou, E.; Siotis, I.; Vayaki, A.; Blondel, A.; Bonneaud, G.; Brient, J. C.; Bourdon, P.; Rougé, A.; Rumpf, M.; Valassi, A.; Verderi, M.; Videau, H.; Candlin, D. J.; Parsons, M. I.; Focardi, E.; Parrini, G.; Zachariadou, K.; Corden, M.; Georgiopoulos, C.; Jaffe, D. E.; Antonelli, A.; Bencivenni, G.; Bologna, G.; Bossi, F.; Campana, P.; Capon, G.; Casper, D.; Chiarella, V.; Felici, G.; Laurelli, P.; Mannocchi, G.; Murtas, F.; Murtas, G. P.; Passalacqua, L.; Pepe-Altarelli, M.; Curtis, L.; Dorris, S. J.; Halley, A. W.; Knowles, I. G.; Lynch, J. G.; O'Shea, V.; Raine, C.; Scarr, J. M.; Smith, K.; Teixeira-Dias, P.; Thompson, A. S.; Thomson, E.; Thomson, F.; Turnbull, R. M.; Geweniger, C.; Graefe, G.; Hanke, P.; Hansper, G.; Hepp, V.; Kluge, E. E.; Putzer, A.; Schmidt, M.; Sommer, J.; Tittel, K.; Werner, S.; Wunsch, M.; Beuselinck, R.; Binnie, D. M.; Cameron, W.; Dornan, P. J.; Girone, M.; Goodsir, S.; Martin, E. B.; Moutoussi, A.; Nash, J.; Sedgbeer, J. K.; Stacey, A. M.; Williams, M. D.; Dissertori, G.; Ghete, V. M.; Girtler, P.; Kuhn, D.; Rudolph, G.; Betteridge, A. P.; Bowdery, C. K.; Colrain, P.; Crawford, G.; Finch, A. J.; Foster, F.; Hughes, G.; Sloan, T.; Williams, M. I.; Galla, A.; Giehl, I.; Greene, A. M.; Hoffmann, C.; Jakobs, K.; Kleinknecht, K.; Quast, G.; Renk, B.; Rohne, E.; Sander, H.-G.; van Gemmeren, P.; Zeitnitz, C.; Aubert, J. J.; Benchouk, C.; Bonissent, A.; Bujosa, G.; Calvet, D.; Carr, J.; Coyle, P.; Diaconu, C.; Etienne, F.; Konstantinidis, N.; Leroy, O.; Motsch, F.; Payre, P.; Rousseau, D.; Talby, M.; Sadouki, A.; Thulasidas, M.; Trabelsi, K.; Aleppo, M.; Ragusa, F.; Berlich, R.; Blum, W.; Brown, D.; Büscher, V.; Dietl, H.; Dydak, F.; Ganis, G.; Gotzhein, C.; Kroha, H.; Lütjens, G.; Lutz, G.; Männer, W.; Moser, H.-G.; Richter, R.; Rosado-Schlosser, A.; Schael, S.; Settles, R.; Seywerd, H.; St. Denis, R.; Stenzel, H.; Wiedenmann, W.; Wolf, G.; Boucrot, J.; Callot, O.; Chen, S.; Choi, Y.; Cordier, A.; Davier, M.; Duflot, L.; Grivaz, J.-F.; Heusse, Ph.; Höcker, A.; Jacholkowska, A.; Jacquet, M.; Kim, D. W.; Le Diberder, F.; Lefrançois, J.; Lutz, A.-M.; Nilolic, I.; Schune, M.-H.; Simion, S.; Tournefier, E.; Veillet, J.-J.; Videau, I.; Zerwas, D.; Azzurri, P.; Bagliesi, G.; Batignani, G.; Bettarini, S.; Bozzi, C.; Calderini, G.; Carpinelli, M.; Ciocci, M. A.; Ciulli, V.; dell'Orso, R.; Fantechi, R.; Ferrante, I.; Foà, L.; Forti, F.; Giassi, A.; Giorgi, M. A.; Gregorio, A.; Ligabue, F.; Lusiani, A.; Marrocchesi, P. S.; Messineo, A.; Palla, F.; Sanguinetti, G.; Sciabà, A.; Spagnolo, P.; Steinberger, J.; Tenchini, R.; Tonelli, G.; Vannini, C.; Venturi, A.; Verdini, P. G.; Blair, G. A.; Bryant, L. M.; Chambers, J. T.; Gao, Y.; Green, M. G.; Medcalf, T.; Perrodo, P.; Strong, J. A.; von Wimmersperg-Toeller, J. H.; Botterill, D. R.; Clifft, R. W.; Edgecock, T. R.; Haywood, S.; Maley, P.; Norton, P. R.; Thompson, J. C.; Wright, A. E.; Bloch-Devaux, B.; Colas, P.; Emery, S.; Kozanecki, W.; Lançon, E.; Lemaire, M. C.; Locci, E.; Perez, P.; Rander, J.; Renardy, J.-F.; Roussarie, A.; Schuller, J.-P.; Schwindling, J.; Trabelsi, A.; Vallage, B.; Black, S. N.; Dann, J. H.; Johnson, R. P.; Kim, H. Y.; Litke, A. M.; McNeil, M. A.; Taylor, G.; Booth, C. N.; Boswell, R.; Brew, C. A. J.; Cartwright, S.; Combley, F.; Kelly, M. S.; Lehto, M.; Newton, W. M.; Reeve, J.; Thompson, L. F.; Böhrer, A.; Brandt, S.; Cowan, G.; Grupen, C.; Saraiva, P.; Smolik, L.; Stephan, F.; Apollonio, M.; Bosisio, L.; Della Marina, R.; Giannini, G.; Gobbo, B.; Musolino, G.; Rothberg, J.; Wasserbaech, S.; Armstrong, S. R.; Charles, E.; Elmer, P.; Ferguson, D. P. S.; Gao, Y. S.; González, S.; Greening, T. C.; Hayes, O. J.; Hu, H.; Jin, S.; McNamara, P. A.; Nachtman, J. M.; Nielsen, J.; Orejudos, W.; Pan, Y. B.; Saadi, Y.; Scott, I. J.; Walsh, J.; Sau, Lan Wu; Wu, X.; Yamartino, J. M.; Zobernig, G.

    1997-02-01

    A measurement of Rb using five mutually exclusive hemisphere tags has been performed by ALEPH using the full LEP1 statistics. Three tags are designed to select the decay of the Z0 to b quarks, while the remaining two selectZ0 decays to c and light quarks, and are used to measure the tagging efficiencies. The result, Rb = 0.2159 +/- 0.0009 (stat) +/- 0.0011 (syst), is in agreement with the electroweak theory prediction of 0.2158 +/- 0.0003.

  10. A Measurement of $R_b$ using Mutually Exclusive Tags

    CERN Document Server

    Barate, R; Décamp, D; Ghez, P; Goy, C; Lees, J P; Lucotte, A; Minard, M N; Nief, J Y; Pietrzyk, B; Casado, M P; Chmeissani, M; Comas, P; Crespo, J M; Delfino, M C; Fernández, E; Fernández-Bosman, M; Garrido, L; Juste, A; Martínez, M; Miquel, R; Mir, L M; Orteu, S; Padilla, C; Park, I C; Pascual, A; Perlas, J A; Riu, I; Sánchez, F; Teubert, F; Colaleo, A; Creanza, D; De Palma, M; Gelao, G; Iaselli, Giuseppe; Maggi, G; Maggi, M; Marinelli, N; Nuzzo, S; Ranieri, A; Raso, G; Ruggieri, F; Selvaggi, G; Silvestris, L; Tempesta, P; Tricomi, A; Zito, G; Huang, X; Lin, J; Ouyang, Q; Wang, T; Xie, Y; Xu, R; Xue, S; Zhang, J; Zhang, L; Zhao, W; Abbaneo, D; Alemany, R; Becker, U; Bazarko, A O; Bright-Thomas, P G; Cattaneo, M; Cerutti, F; Drevermann, H; Forty, Roger W; Frank, M; Hagelberg, R; Harvey, J; Janot, P; Jost, B; Kneringer, E; Knobloch, J; Lehraus, Ivan; Lutters, G; Mato, P; Minten, Adolf G; Moneta, L; Pacheco, A; Pusztaszeri, J F; Ranjard, F; Rensing, P E; Rizzo, G; Rolandi, Luigi; Schlatter, W D; Schmitt, M; Schneider, O; Tejessy, W; Tomalin, I R; Wachsmuth, H W; Wagner, A; Ajaltouni, Ziad J; Barrès, A; Boyer, C; Falvard, A; Ferdi, C; Gay, P; Guicheney, C; Henrard, P; Jousset, J; Michel, B; Monteil, S; Montret, J C; Pallin, D; Perret, P; Podlyski, F; Proriol, J; Rosnet, P; Rossignol, J M; Fearnley, Tom; Hansen, J B; Hansen, J D; Hansen, J R; Hansen, P H; Nilsson, B S; Rensch, B; Wäänänen, A; Daskalakis, G; Kyriakis, A; Markou, C; Simopoulou, Errietta; Siotis, I; Vayaki, Anna; Blondel, A; Bonneaud, G R; Brient, J C; Bourdon, P; Rougé, A; Rumpf, M; Valassi, Andrea; Verderi, M; Videau, H L; Candlin, D J; Parsons, M I; Focardi, E; Parrini, G; Zachariadou, K; Corden, M; Georgiopoulos, C H; Jaffe, D E; Antonelli, A; Bencivenni, G; Bologna, G; Bossi, F; Campana, P; Capon, G; Casper, David William; Chiarella, V; Felici, G; Laurelli, P; Mannocchi, G; Murtas, F; Murtas, G P; Passalacqua, L; Pepé-Altarelli, M; Curtis, L; Dorris, S J; Halley, A W; Knowles, I G; Lynch, J G; O'Shea, V; Raine, C; Scarr, J M; Smith, K; Teixeira-Dias, P; Thompson, A S; Thomson, E; Thomson, F; Turnbull, R M; Geweniger, C; Graefe, G; Hanke, P; Hansper, G; Hepp, V; Kluge, E E; Putzer, A; Schmidt, M; Sommer, J; Tittel, K; Werner, S; Wunsch, M; Beuselinck, R; Binnie, David M; Cameron, W; Dornan, Peter J; Girone, M; Goodsir, S M; Martin, E B; Moutoussi, A; Nash, J; Sedgbeer, J K; Stacey, A M; Williams, M D; Dissertori, G; Ghete, V M; Girtler, P; Kuhn, D; Rudolph, G; Betteridge, A P; Bowdery, C K; Colrain, P; Crawford, G; Finch, A J; Foster, F; Hughes, G; Sloan, Terence; Williams, M I; Galla, A; Giehl, I; Greene, A M; Hoffmann, C; Jakobs, K; Kleinknecht, K; Quast, G; Renk, B; Rohne, E; Sander, H G; Van Gemmeren, P; Zeitnitz, C; Aubert, Jean-Jacques; Benchouk, C; Bonissent, A; Bujosa, G; Calvet, D; Carr, J; Coyle, P; Diaconu, C A; Etienne, F; Konstantinidis, N P; Leroy, O; Motsch, F; Payre, P; Rousseau, D; Talby, M; Sadouki, A; Thulasidas, M; Trabelsi, K; Aleppo, M; Ragusa, F; Berlich, R; Blum, Walter; Brown, D; Büscher, V; Dietl, H; Dydak, Friedrich; Ganis, G; Gotzhein, C; Kroha, H; Lütjens, G; Lutz, Gerhard; Männer, W; Moser, H G; Richter, R H; Rosado-Schlosser, A; Schael, S; Settles, Ronald; Seywerd, H C J; Saint-Denis, R; Stenzel, H; Wiedenmann, W; Wolf, G; Boucrot, J; Callot, O; Chen, S; Choi, Y; Cordier, A; Davier, M; Duflot, L; Grivaz, J F; Heusse, P; Höcker, A; Jacholkowska, A; Jacquet, M; Kim, D W; Le Diberder, F R; Lefrançois, J; Lutz, A M; Nikolic, I A; Schune, M H; Simion, S; Tournefier, E; Veillet, J J; Videau, I; Zerwas, D; Azzurri, P; Bagliesi, G; Batignani, G; Bettarini, S; Bozzi, C; Calderini, G; Carpinelli, M; Ciocci, M A; Ciulli, V; Dell'Orso, R; Fantechi, R; Ferrante, I; Foà, L; Forti, F; Giassi, A; Giorgi, M A; Gregorio, A; Ligabue, F; Lusiani, A; Marrocchesi, P S; Messineo, A; Palla, Fabrizio; Sanguinetti, G; Sciabà, A; Spagnolo, P; Steinberger, Jack; Tenchini, Roberto; Tonelli, G; Vannini, C; Venturi, A; Verdini, P G; Blair, G A; Bryant, L M; Chambers, J T; Gao, Y; Green, M G; Medcalf, T; Perrodo, P; Strong, J A; Von Wimmersperg-Töller, J H; Botterill, David R; Clifft, R W; Edgecock, T R; Haywood, S; Maley, P; Norton, P R; Thompson, J C; Wright, A E; Bloch-Devaux, B; Colas, P; Emery, S; Kozanecki, Witold; Lançon, E; Lemaire, M C; Locci, E; Pérez, P; Rander, J; Renardy, J F; Roussarie, A; Schuller, J P; Schwindling, J; Trabelsi, A; Vallage, B; Black, S N; Dann, J H; Johnson, R P; Kim, H Y; Litke, A M; McNeil, M A; Taylor, G; Booth, C N; Boswell, R; Brew, C A J; Cartwright, S L; Combley, F; Kelly, M S; Lehto, M H; Newton, W M; Reeve, J; Thompson, L F; Böhrer, A; Brandt, S; Cowan, G D; Grupen, Claus; Saraiva, P; Smolik, L; Stephan, F; Apollonio, M; Bosisio, L; Della Marina, R; Giannini, G; Gobbo, B; Musolino, G; Rothberg, J E; Wasserbaech, S R; Armstrong, S R; Charles, E; Elmer, P; Ferguson, D P S; Gao, Y S; González, S; Greening, T C; Hayes, O J; Hu, H; Jin, S; McNamara, P A; Nachtman, J M; Nielsen, J; Orejudos, W; Pan, Y B; Saadi, Y; Scott, I J; Walsh, J; Wu Sau Lan; Wu, X; Yamartino, J M; Zobernig, G

    1997-01-01

    A measurement of $R_b$ using five mutually exclusive hemisphere tags has been pe rformed by ALEPH using the full LEP1 statistics. Three tags are designed to sele ct the decay of the $Z^0$ to $b$ quarks, while the remaining two select $Z^0$ decays to $c$ and light quarks, and are used to measure the tagging efficiencies. The result, {$R_b~=~0.2159~\\pm~0.0009\\mbox{(stat)}~\\pm 0.0011\\mbox{(syst)}$}, is in agreement with the electroweak theory prediction of $0.2158 \\pm 0.0003$.

  11. Efficient and robust quantum random number generation by photon number detection

    International Nuclear Information System (INIS)

    Applegate, M. J.; Thomas, O.; Dynes, J. F.; Yuan, Z. L.; Shields, A. J.; Ritchie, D. A.

    2015-01-01

    We present an efficient and robust quantum random number generator based upon high-rate room temperature photon number detection. We employ an electric field-modulated silicon avalanche photodiode, a type of device particularly suited to high-rate photon number detection with excellent photon number resolution to detect, without an applied dead-time, up to 4 photons from the optical pulses emitted by a laser. By both measuring and modeling the response of the detector to the incident photons, we are able to determine the illumination conditions that achieve an optimal bit rate that we show is robust against variation in the photon flux. We extract random bits from the detected photon numbers with an efficiency of 99% corresponding to 1.97 bits per detected photon number yielding a bit rate of 143 Mbit/s, and verify that the extracted bits pass stringent statistical tests for randomness. Our scheme is highly scalable and has the potential of multi-Gbit/s bit rates

  12. Combined DECS Analysis and Next-Generation Sequencing Enable Efficient Detection of Novel Plant RNA Viruses.

    Science.gov (United States)

    Yanagisawa, Hironobu; Tomita, Reiko; Katsu, Koji; Uehara, Takuya; Atsumi, Go; Tateda, Chika; Kobayashi, Kappei; Sekine, Ken-Taro

    2016-03-07

    The presence of high molecular weight double-stranded RNA (dsRNA) within plant cells is an indicator of infection with RNA viruses as these possess genomic or replicative dsRNA. DECS (dsRNA isolation, exhaustive amplification, cloning, and sequencing) analysis has been shown to be capable of detecting unknown viruses. We postulated that a combination of DECS analysis and next-generation sequencing (NGS) would improve detection efficiency and usability of the technique. Here, we describe a model case in which we efficiently detected the presumed genome sequence of Blueberry shoestring virus (BSSV), a member of the genus Sobemovirus, which has not so far been reported. dsRNAs were isolated from BSSV-infected blueberry plants using the dsRNA-binding protein, reverse-transcribed, amplified, and sequenced using NGS. A contig of 4,020 nucleotides (nt) that shared similarities with sequences from other Sobemovirus species was obtained as a candidate of the BSSV genomic sequence. Reverse transcription (RT)-PCR primer sets based on sequences from this contig enabled the detection of BSSV in all BSSV-infected plants tested but not in healthy controls. A recombinant protein encoded by the putative coat protein gene was bound by the BSSV-antibody, indicating that the candidate sequence was that of BSSV itself. Our results suggest that a combination of DECS analysis and NGS, designated here as "DECS-C," is a powerful method for detecting novel plant viruses.

  13. Efficient Hybrid Detection of Node Replication Attacks in Mobile Sensor Networks

    Directory of Open Access Journals (Sweden)

    Ze Wang

    2017-01-01

    Full Text Available The node replication attack is one of the notorious attacks that can be easily launched by adversaries in wireless sensor networks. A lot of literatures have studied mitigating the node replication attack in static wireless sensor networks. However, it is more difficult to detect the replicas in mobile sensor networks because of their node mobility. Considering the limitations of centralized detection schemes for static wireless sensor networks, a few distributed solutions have been recently proposed. Some existing schemes identified replicated attacks by sensing mobile nodes with identical ID but different locations. To facilitate the discovery of contradictory conflicts, we propose a hybrid local and global detection method. The local detection is performed in a local area smaller than the whole deployed area to improve the meeting probability of contradictory nodes, while the distant replicated nodes in larger area can also be efficiently detected by the global detection. The complementary two levels of detection achieve quick discovery by searching of the replicas with reasonable overhead.

  14. An efficient detection of epileptic seizure by differentiation and spectral analysis of electroencephalograms.

    Science.gov (United States)

    Kang, Jae-Hwan; Chung, Yoon Gi; Kim, Sung-Phil

    2015-11-01

    Epilepsy is a critical neurological disorder resulting from abnormal hyper-excitability of neurons in the brain. Studies have shown that epilepsy can be detected in electroencephalography (EEG) recordings of patients suffering from seizures. The performance of EEG-based epileptic seizure detection relies largely on how well one can extract features from an EEG that characterize seizure activity. Conventional feature extraction methods using time-series analysis, spectral analysis and nonlinear dynamic analysis have advanced in recent years to improve detection. The computational complexity has also increased to obtain a higher detection rate. This study aimed to develop an efficient feature extraction method based on Hjorth's mobility to reduce computational complexity while maintaining high detection accuracy. A new feature extraction method was proposed by computing the spectral power of Hjorth's mobility components, which were effectively estimated by differentiating EEG signals in real-time. Using EEG data in five epileptic patients, this method resulted in a detection rate of 99.46% between interictal and epileptic EEG signals and 99.78% between normal and epileptic EEG signals, which is comparable to most advanced nonlinear methods. These results suggest that the spectral features of Hjorth's mobility components in EEG signals can represent seizure activity and may pave the way for developing a fast and reliable epileptic seizure detection method. Copyright © 2015 Elsevier Ltd. All rights reserved.

  15. An efficient abnormal cervical cell detection system based on multi-instance extreme learning machine

    Science.gov (United States)

    Zhao, Lili; Yin, Jianping; Yuan, Lihuan; Liu, Qiang; Li, Kuan; Qiu, Minghui

    2017-07-01

    Automatic detection of abnormal cells from cervical smear images is extremely demanded in annual diagnosis of women's cervical cancer. For this medical cell recognition problem, there are three different feature sections, namely cytology morphology, nuclear chromatin pathology and region intensity. The challenges of this problem come from feature combination s and classification accurately and efficiently. Thus, we propose an efficient abnormal cervical cell detection system based on multi-instance extreme learning machine (MI-ELM) to deal with above two questions in one unified framework. MI-ELM is one of the most promising supervised learning classifiers which can deal with several feature sections and realistic classification problems analytically. Experiment results over Herlev dataset demonstrate that the proposed method outperforms three traditional methods for two-class classification in terms of well accuracy and less time.

  16. Kepler Planet Detection Metrics: Pixel-Level Transit Injection Tests of Pipeline Detection Efficiency for Data Release 25

    Science.gov (United States)

    Christiansen, Jessie L.

    2017-01-01

    This document describes the results of the fourth pixel-level transit injection experiment, which was designed to measure the detection efficiency of both the Kepler pipeline (Jenkins 2002, 2010; Jenkins et al. 2017) and the Robovetter (Coughlin 2017). Previous transit injection experiments are described in Christiansen et al. (2013, 2015a,b, 2016).In order to calculate planet occurrence rates using a given Kepler planet catalogue, produced with a given version of the Kepler pipeline, we need to know the detection efficiency of that pipeline. This can be empirically determined by injecting a suite of simulated transit signals into the Kepler data, processing the data through the pipeline, and examining the distribution of successfully recovered transits. This document describes the results for the pixel-level transit injection experiment performed to accompany the final Q1-Q17 Data Release 25 (DR25) catalogue (Thompson et al. 2017)of the Kepler Objects of Interest. The catalogue was generated using the SOC pipeline version 9.3 and the DR25 Robovetter acting on the uniformly processed Q1-Q17 DR25 light curves (Thompson et al. 2016a) and assuming the Q1-Q17 DR25 Kepler stellar properties (Mathur et al. 2017).

  17. LHCb Tag Collector

    CERN Document Server

    Fuente Fernàndez, P; Cousin, N

    2011-01-01

    The LHCb physics software consists of hundreds of packages, each of which is developed by one or more physicists. When the developers have some code changes that they would like released, they commit them to the version control system, and enter the revision number into a database. These changes have to be integrated into a new release of each of the physics analysis applications. Tests are then performed by a nightly build system, which rebuilds various configurations of the whole software stack and executes a suite of run-time functionality tests. A Tag Collector system has been developed using solid standard technologies to cover both the use cases of developers and integration managers. A simple Web interface, based on an AJAX-like technology, is available. Integration with software management and Nightly Build programs is possible via a Python API. Data are stored in a relational database with the help of an ORM (Object-Relational Mapping) library.

  18. Quantifying Tourist Behavior Patterns by Travel Motifs and Geo-Tagged Photos from Flickr

    Directory of Open Access Journals (Sweden)

    Liu Yang

    2017-11-01

    Full Text Available With millions of people traveling to unfamiliar cities to spend holidays, travel recommendation becomes necessary to assist tourists in planning their trips more efficiently. Serving as a prerequisite to travel recommender systems, understanding tourist behavior patterns is therefore of great importance. Recently, geo-tagged photos on social media platforms like Flickr have provided a rich data source that captures location histories of tourists and reflects their preferences. This article utilizes geo-tagged photos from Flickr to extract trajectories of tourists and then extends the concept of motifs from topological spaces, to temporal spaces and to semantic spaces, for detecting tourist mobility patterns. By representing trajectories in terms of three distinct types of travel motif and further using them to measure user similarity, typical tourist travel behavior patterns associated with distinct sightseeing tastes/preferences are identified and analyzed for tourism recommendation. Our empirical results confirm that the proposed analytical framework is effective to uncover meaningful tourist behavior patterns.

  19. Engineering a novel multifunctional green fluorescent protein tag for a wide variety of protein research.

    Directory of Open Access Journals (Sweden)

    Takuya Kobayashi

    Full Text Available BACKGROUND: Genetically encoded tag is a powerful tool for protein research. Various kinds of tags have been developed: fluorescent proteins for live-cell imaging, affinity tags for protein isolation, and epitope tags for immunological detections. One of the major problems concerning the protein tagging is that many constructs with different tags have to be made for different applications, which is time- and resource-consuming. METHODOLOGY/PRINCIPAL FINDINGS: Here we report a novel multifunctional green fluorescent protein (mfGFP tag which was engineered by inserting multiple peptide tags, i.e., octa-histidine (8xHis, streptavidin-binding peptide (SBP, and c-Myc tag, in tandem into a loop of GFP. When fused to various proteins, mfGFP monitored their localization in living cells. Streptavidin agarose column chromatography with the SBP tag successfully isolated the protein complexes in a native form with a high purity. Tandem affinity purification (TAP with 8xHis and SBP tags in mfGFP further purified the protein complexes. mfGFP was clearly detected by c-Myc-specific antibody both in immunofluorescence and immuno-electron microscopy (EM. These findings indicate that mfGFP works well as a multifunctional tag in mammalian cells. The tag insertion was also successful in other fluorescent protein, mCherry. CONCLUSIONS AND SIGNIFICANCE: The multifunctional fluorescent protein tag is a useful tool for a wide variety of protein research, and may have the advantage over other multiple tag systems in its higher expandability and compatibility with existing and future tag technologies.

  20. Proton and hydrogen atom detection efficiency of resistance strip magnetic electron multiplier particle-counting system

    Energy Technology Data Exchange (ETDEWEB)

    Wehrenberg, P.J.; Clark, K.C.

    1976-10-01

    The absolute detection efficiency for protons and for hydrogen atoms in the energy range 5--60 keV is determined for a resistance strip magnetic electron multiplier particle-counting system. Significant history-dependent gain variations are discussed. The detector system is suitable for use in coincidence experiments requiring particle-counting rates to 1.0 MHz and timing accuracies of 3.0 nsec. (AIP)

  1. An improved accelerated frame slotted aloha (afsa) algorithm for tag collision in rfid

    OpenAIRE

    Ahmad, Tanveer; feng, Yan tian

    2014-01-01

    The efficiency of tag identification in an RFID system can be low down due to the tag collision problems. the tag collision problem occurs when a reader try to read multiple tags in an interrogation zone. as a result the reader does not identify the tag correctly. that causes a loss of information or data interference. to solve such kind of issues a series of aloha based algorithm and binary search algorithm have beed proposed. the most simple popular and good giving performance algorithm are...

  2. Device-free object tracking using passive tags

    CERN Document Server

    Han, Jinsong; Zhao, Kun; Jiang, Zhiping

    2014-01-01

    This SpringerBrief examines the use of cheap commercial passive RFID tags to achieve accurate device-free object-tracking. It presents a sensitive detector, named Twins, which uses a pair of adjacent passive tags to detect uncooperative targets (such as intruders). Twins leverages a newly observed phenomenon called critical state that is caused by interference among passive tags.The author expands on the previous object tracking methods, which are mostly device-based, and reveals a new interference model and their extensive experiments for validation. A prototype implementation of the Twins-ba

  3. Antibody tagged gold nanoparticles as scattering probes for the pico molar detection of the proteins in blood serum using nanoparticle tracking analyzer.

    Science.gov (United States)

    Kashid, Sahebrao Balaso; Tak, Rajesh D; Raut, Rajesh Warluji

    2015-09-01

    We report a rapid one-step immunoassay to detect protein using antibody conjugated gold nanoparticles (AbGNPs) where the targeted protein concentration was determined by analyzing the gold nanoparticle aggregation caused by antibody-antigen interactions using nanoparticles tracking analysis (NTA) technique. The sandwich structure constituting the binding of the targeted human IgG to the gold nanoparticle conjugates with goat anti human monoclonal IgG (AbGNPs) was confirmed by transmission electron microscopy. The binding of human IgG (antigen, mentioned hence forth as AT) induce AbGNPs to form dimers or trimers through a typical antibody-antigen-antibody sandwich structure that can be analyzed for the sensitive determination on the basis of change in hydrodynamic diameter of AbGNPs. By this method the minimum detectable concentration of AT is found to be below 2pg/ml. We expect that a significant change in the hydrodynamic diameter of AbGNP could form the basis for the rapid one-step immunoassay development. Copyright © 2015 Elsevier B.V. All rights reserved.

  4. Study of the photo-detection efficiency of FBK High-Density silicon photomultipliers

    International Nuclear Information System (INIS)

    Zappalà, G.; Regazzoni, V.; Acerbi, F.; Ferri, A.; Gola, A.; Paternoster, G.; Zorzi, N.; Piemonte, C.

    2016-01-01

    This work presents a study of the factors contributing to the Photo-Detection Efficiency of Silicon Photomultipliers (SiPMs): Quantum Efficiency, Triggering Probability and Fill Factor. Two different SiPM High-Density technologies are tested, NUV-HD, based on n-on-p junction, and RGB-HD, based on p-on-n junction, developed at FBK, Trento. The quantum efficiency was measured on photodiodes produced along with the SiPMs. The triggering probability, as a function of wavelength and bias voltage, was measured on circular Single Photon Avalanche Diodes (SPADs) with 100% fill factor. Square SPADs, having the same layout of single SiPM cells, were studied to measure the effective fill factor and compare it to the nominal value. The comparison of the circular and square SPADs allows to get the transition region size between the effective active area of the cell and the one defined by the layout.

  5. Development of a novel fluorescent tag O-2-[aminoethyl]fluorescein for the electrophoretic separation of oligosaccharides

    International Nuclear Information System (INIS)

    Kazarian, Artaches A.; Smith, Jason A.; Hilder, Emily F.; Breadmore, Michael C.; Quirino, Joselito P.; Suttil, James

    2010-01-01

    This study describes the development of a novel fluorescent tag, O-2-[aminoethyl]fluorescein, for the separation of sugars by capillary electrophoresis with fluorescence detection using an argon ion laser. The tag was synthesised using three consecutive steps namely: esterification, alkylation and hydrolysis, specifically designed to offer a flexible way in which to make an assortment of fluorescent tags from cheap and readily available starting reagents (typically less than $1 per g of fluorescent tag). Via this flexible synthetic pathway, O-2-[aminoethyl]fluorescein was designed and synthesised with a spacer group to lower steric effects between the fluorescein backbone and the reducing end of the carbohydrate which were anticipated to improve the reactivity of the tag. The newly synthesised tag, O-2-[aminoethyl]fluorescein was evaluated against structurally similar commercial fluorescent motifs namely fluorescent 5-aminomethylfluorescein and non-fluorescent 5-aminofluorescein. Kinetic studies indicated that O-2-[aminoethyl]fluorescein showed similar labeling efficiencies as 5-aminomethylfluorescein, but were achieved in only 30 min, supporting the notion of improved reactivity of the spacer group. The sensitivity of O-2-[aminoethyl]fluorescein was evaluated using maltoheptaose with a detection limit of 1 nM obtained, which was slightly higher than that of 0.3 nM obtained with 5-aminomethylfluorescein, and was due to its lower quantum yield (0.24) when conjugated to the sugar. The separation performance of the tag was also benchmarked with the two commercial reagents using a range of corn syrup oligosaccharides, from 4 to 10 glucose units, typically found in rice starch. Separations were performed using an electrolyte containing 100 mM boric acid, tris at pH 8.65 as background electrolyte, 30 kV applied voltage, 50 μm I.D. x 40 cm (30 cm effective length) capillary. The novel tag showed better resolution of small oligosaccharides, G3 and G4, than the other two

  6. Sensitive Western-Blot Analysis of Azide-Tagged Protein Post Translational Modifications Using Thermoresponsive Polymer Self-Assembly.

    Science.gov (United States)

    Liu, Tong; Zhang, Wanjun; Zhang, Zheng; Chen, Mingli; Wang, Jianhua; Qian, Xiaohong; Qin, Weijie

    2018-02-06

    Western-blot (WB) is a powerful analytical technique for protein identification in complex biological samples and has been widely used in biological studies for decades. Detection specificity and sensitivity of WB largely relies on quality of the antibodies and performance of the conjugated HRP. However, the application of WB analysis for the detection of protein post-translational modifications (PTMs) is hampered by the low abundance of protein PTMs and by the limited availability of antibodies that specifically differentiate various kinds of PTMs from their protein substrates. Therefore, new recognition mechanisms and signal amplification strategies for WB analysis of protein PTMs is in high demand. In this work, we prepared a soluble polymer that detects various azide-tagged PTM proteins in WB analysis using triarylphosphine and HRP modified thermoresponsive polymer. Specific and efficient detection of azide-tagged PTM protein is achieved via the bioorthogonal reaction between azide and triarylphosphine. More importantly, the chemiluminiscent signal in the WB analysis is largely amplified by the temperature induced self-assembly of numerous thermoresponsive polymer chains carrying multiple HRPs. As a result, approximately 100 times more sensitive detection than commercial antibodies is achieved by this method using standard PTM proteins. Though, this new reagent does not directly detect native PTMs in cell, tissue or blood samples, it still has important application potential in protein PTM studies, considering the wide availability of azide-tagging techniques to a variety of PTMs.

  7. Semiotic dynamics and collaborative tagging.

    Science.gov (United States)

    Cattuto, Ciro; Loreto, Vittorio; Pietronero, Luciano

    2007-01-30

    Collaborative tagging has been quickly gaining ground because of its ability to recruit the activity of web users into effectively organizing and sharing vast amounts of information. Here we collect data from a popular system and investigate the statistical properties of tag cooccurrence. We introduce a stochastic model of user behavior embodying two main aspects of collaborative tagging: (i) a frequency-bias mechanism related to the idea that users are exposed to each other's tagging activity; (ii) a notion of memory, or aging of resources, in the form of a heavy-tailed access to the past state of the system. Remarkably, our simple modeling is able to account quantitatively for the observed experimental features with a surprisingly high accuracy. This points in the direction of a universal behavior of users who, despite the complexity of their own cognitive processes and the uncoordinated and selfish nature of their tagging activity, appear to follow simple activity patterns.

  8. Engineering the ATLAS TAG Browser

    CERN Document Server

    Zhang, Q; The ATLAS collaboration

    2011-01-01

    ELSSI is a web-based event metadata (TAG) browser and event-level selection service for ATLAS. TAGs from all ATLAS physics and Monte Carlo data sets are routinely loaded into Oracle databases as an integral part of event processing. As data volumes increase, more and more sites are joining the distributed TAG data hosting topology. Meanwhile, TAG content and database schemata continue to evolve as new user requirements and additional sources of metadata emerge. All of this has posed many challenges to the development of ELSSI, which must support vast amounts of TAG data while source, content, geographic locations, and user query patterns may change over time. In this paper, we describe some of the challenges encountered in the process of developing ELSSI, and the software engineering strategies adopted to address those challenges. Approaches to management of access to data, browsing, data rendering, query building, query validation, execution, connection management, and communication with auxiliary services a...

  9. Accuracy and efficiency of detection dogs: a powerful new tool for koala conservation and management.

    Science.gov (United States)

    Cristescu, Romane H; Foley, Emily; Markula, Anna; Jackson, Gary; Jones, Darryl; Frère, Céline

    2015-02-10

    Accurate data on presence/absence and spatial distribution for fauna species is key to their conservation. Collecting such data, however, can be time consuming, laborious and costly, in particular for fauna species characterised by low densities, large home ranges, cryptic or elusive behaviour. For such species, including koalas (Phascolarctos cinereus), indicators of species presence can be a useful shortcut: faecal pellets (scats), for instance, are widely used. Scat surveys are not without their difficulties and often contain a high false negative rate. We used experimental and field-based trials to investigate the accuracy and efficiency of the first dog specifically trained for koala scats. The detection dog consistently out-performed human-only teams. Off-leash, the dog detection rate was 100%. The dog was also 19 times more efficient than current scat survey methods and 153% more accurate (the dog found koala scats where the human-only team did not). This clearly demonstrates that the use of detection dogs decreases false negatives and survey time, thus allowing for a significant improvement in the quality and quantity of data collection. Given these unequivocal results, we argue that to improve koala conservation, detection dog surveys for koala scats could in the future replace human-only teams.

  10. ELD-Net: An efficient deep learning architecture for accurate saliency detection.

    Science.gov (United States)

    Lee, Gayoung; Tai, Yu-Wing; Kim, Junmo

    2017-08-09

    Recent advances in saliency detection have utilized deep learning to obtain high-level features to detect salient regions in scenes. In this paper, we propose ELD-Net, a unified deep learning framework for accurate and efficient saliency detection. We show that hand-crafted features can provide complementary information to enhance saliency detection that uses only high-level features. Our method uses both low-level and high-level features for saliency detection. High-level features are extracted using GoogLeNet, and low-level features evaluate the relative importance of a local region using its differences from other regions in an image. The two feature maps are independently encoded by the convolutional and the ReLU layers. The encoded low-level and high-level features are then combined by concatenation and convolution. Finally, a linear fully connected layer is used to evaluate the saliency of a queried region. A full resolution saliency map is obtained by querying the saliency of each local region of an image. Since the high-level features are encoded at low resolution, and the encoded high-level features can be reused for every query region, our ELD-Net is very fast. Our experiments show that our method outperforms state-of-the-art deep learning-based saliency detection methods.

  11. Efficient pedestrian detection from aerial vehicles with object proposals and deep convolutional neural networks

    Science.gov (United States)

    Minnehan, Breton; Savakis, Andreas

    2016-05-01

    As Unmanned Aerial Systems grow in numbers, pedestrian detection from aerial platforms is becoming a topic of increasing importance. By providing greater contextual information and a reduced potential for occlusion, the aerial vantage point provided by Unmanned Aerial Systems is highly advantageous for many surveillance applications, such as target detection, tracking, and action recognition. However, due to the greater distance between the camera and scene, targets of interest in aerial imagery are generally smaller and have less detail. Deep Convolutional Neural Networks (CNN's) have demonstrated excellent object classification performance and in this paper we adopt them to the problem of pedestrian detection from aerial platforms. We train a CNN with five layers consisting of three convolution-pooling layers and two fully connected layers. We also address the computational inefficiencies of the sliding window method for object detection. In the sliding window configuration, a very large number of candidate patches are generated from each frame, while only a small number of them contain pedestrians. We utilize the Edge Box object proposal generation method to screen candidate patches based on an "objectness" criterion, so that only regions that are likely to contain objects are processed. This method significantly reduces the number of image patches processed by the neural network and makes our classification method very efficient. The resulting two-stage system is a good candidate for real-time implementation onboard modern aerial vehicles. Furthermore, testing on three datasets confirmed that our system offers high detection accuracy for terrestrial pedestrian detection in aerial imagery.

  12. Efficient Near Maximum-Likelihood Detection for Underdetermined MIMO Antenna Systems Using a Geometrical Approach

    Directory of Open Access Journals (Sweden)

    Arogyaswami Paulraj

    2007-12-01

    Full Text Available Maximum-likelihood (ML detection is guaranteed to yield minimum probability of erroneous detection and is thus of great importance for both multiuser detection and space-time decoding. For multiple-input multiple-output (MIMO antenna systems where the number of receive antennas is at least the number of signals multiplexed in the spatial domain, ML detection can be done efficiently using sphere decoding. Suboptimal detectors are also well known to have reasonable performance at low complexity. It is, nevertheless, much less understood for obtaining good detection at affordable complexity if there are less receive antennas than transmitted signals (i.e., underdetermined MIMO systems. In this paper, our aim is to develop an effcient detection strategy that can achieve near ML performance for underdetermined MIMO systems. Our method is based on the geometrical understanding that the ML point happens to be a point that is “close” to the decoding hyperplane in all directions. The fact that such proximity-close points are much less is used to devise a decoding method that promises to greatly reduce the decoding complexity while achieving near ML performance. An average-case complexity analysis based on Gaussian approximation is also given.

  13. Efficient Near Maximum-Likelihood Detection for Underdetermined MIMO Antenna Systems Using a Geometrical Approach

    Directory of Open Access Journals (Sweden)

    Paulraj Arogyaswami

    2007-01-01

    Full Text Available Maximum-likelihood (ML detection is guaranteed to yield minimum probability of erroneous detection and is thus of great importance for both multiuser detection and space-time decoding. For multiple-input multiple-output (MIMO antenna systems where the number of receive antennas is at least the number of signals multiplexed in the spatial domain, ML detection can be done efficiently using sphere decoding. Suboptimal detectors are also well known to have reasonable performance at low complexity. It is, nevertheless, much less understood for obtaining good detection at affordable complexity if there are less receive antennas than transmitted signals (i.e., underdetermined MIMO systems. In this paper, our aim is to develop an effcient detection strategy that can achieve near ML performance for underdetermined MIMO systems. Our method is based on the geometrical understanding that the ML point happens to be a point that is "close" to the decoding hyperplane in all directions. The fact that such proximity-close points are much less is used to devise a decoding method that promises to greatly reduce the decoding complexity while achieving near ML performance. An average-case complexity analysis based on Gaussian approximation is also given.

  14. Fluorescent QDs-polystyrene composite nanospheres for highly efficient and rapid protein antigen detection

    Energy Technology Data Exchange (ETDEWEB)

    Zhou, Changhua; Mao, Mao [Henan University, Key Laboratory for Special Functional Materials of the Ministry of Education (China); Yuan, Hang [Tsinghua University, Life Science Division, Graduate School at Shenzhen (China); Shen, Huaibin [Henan University, Key Laboratory for Special Functional Materials of the Ministry of Education (China); Wu, Feng; Ma, Lan, E-mail: malan@sz.tsinghua.edu.cn [Tsinghua University, Life Science Division, Graduate School at Shenzhen (China); Li, Lin Song, E-mail: lsli@henu.edu.cn [Henan University, Key Laboratory for Special Functional Materials of the Ministry of Education (China)

    2013-09-15

    In this paper, high-quality carboxyl-functionalized fluorescent (red, green, and blue emitting) nanospheres (46-103 nm) consisting of hydrophobic quantum dots (QDs) and polystyrene were prepared by a miniemulsion polymerization approach. This miniemulsion polymerization approach induced a homogeneous distribution and high aqueous-phase transport efficiency of fluorescent QDs in composite nanospheres, which proved the success of our encoding QDs strategy. The obtained fluorescent nanospheres exhibited high stability in aqueous solution under a wide range of pH, different salt concentrations, PBS buffer, and thermal treatment at 80 Degree-Sign C. Based on the red emitting composite nanosphere, we performed fluorescent lateral flow immunoassay (LFIA) strips for high-sensitivity and rapid alpha-fetal protein detection. The detection limit reached 0.1 ng/mL, which was 200 times higher than commercial colloidal gold-labeled LFIA strips, and it reached similar detection level in enzyme-linked immunosorbent assay kit.

  15. Fluorescent QDs-polystyrene composite nanospheres for highly efficient and rapid protein antigen detection

    Science.gov (United States)

    Zhou, Changhua; Mao, Mao; Yuan, Hang; Shen, Huaibin; Wu, Feng; Ma, Lan; Li, Lin Song

    2013-09-01

    In this paper, high-quality carboxyl-functionalized fluorescent (red, green, and blue emitting) nanospheres (46-103 nm) consisting of hydrophobic quantum dots (QDs) and polystyrene were prepared by a miniemulsion polymerization approach. This miniemulsion polymerization approach induced a homogeneous distribution and high aqueous-phase transport efficiency of fluorescent QDs in composite nanospheres, which proved the success of our encoding QDs strategy. The obtained fluorescent nanospheres exhibited high stability in aqueous solution under a wide range of pH, different salt concentrations, PBS buffer, and thermal treatment at 80 °C. Based on the red emitting composite nanosphere, we performed fluorescent lateral flow immunoassay (LFIA) strips for high-sensitivity and rapid alpha-fetal protein detection. The detection limit reached 0.1 ng/mL, which was 200 times higher than commercial colloidal gold-labeled LFIA strips, and it reached similar detection level in enzyme-linked immunosorbent assay kit.

  16. Measurement of the detection efficiency of the KLOE calorimeter for neutrons between 22 and 174 MeV

    Energy Technology Data Exchange (ETDEWEB)

    Anelli, M. [Laboratori Nazionali di Frascati dell' INFN, Frascati (Italy); Battistoni, G. [Sezione INFN di Milano (Italy); Bertolucci, S. [Laboratori Nazionali di Frascati dell' INFN, Frascati (Italy); Bini, C. [Sapienza Universita di Roma (Italy); Sezione INFN di Roma (Italy); Branchini, P. [Sezione INFN di Roma Tre (Italy); Curceanu, C. [Laboratori Nazionali di Frascati dell' INFN, Frascati (Italy); De Zorzi, G.; Di Domenico, A. [Sapienza Universita di Roma (Italy); Sezione INFN di Roma (Italy); Di Micco, B. [Universita degli di Studi ' Roma Tre' (Italy); Sezione INFN di Roma Tre (Italy); Ferrari, A. [Fondazione CNAO, Milano (Italy); Fiore, S. [Sapienza Universita di Roma (Italy); Sezione INFN di Roma (Italy)], E-mail: salvatore.fiore@roma1.infn.it; Gauzzi, P. [Sapienza Universita di Roma (Italy); Sezione INFN di Roma (Italy); Giovannella, S.; Happacher, F. [Laboratori Nazionali di Frascati dell' INFN, Frascati (Italy); Iliescu, M. [Laboratori Nazionali di Frascati dell' INFN, Frascati (Italy); IFIN-HH, Bucharest (Romania); Martini, M.; Miscetti, S. [Laboratori Nazionali di Frascati dell' INFN, Frascati (Italy); Nguyen, F. [Universita degli di Studi ' Roma Tre' (Italy); Sezione INFN di Roma Tre (Italy); Passeri, A. [Sezione INFN di Roma Tre (Italy); Prokofiev, A. [Svedberg Laboratory, Uppsala University (Sweden)] (and others)

    2009-01-01

    A prototype of the high-sampling lead-scintillating fiber KLOE calorimeter has been exposed to neutron beams of 21, 46 and 174 MeV, provided by the The Svedberg Laboratory, Uppsala, to test its neutron detection efficiency. The measurement of the neutron detection efficiency of an NE110 scintillator provided a reference calibration. The measured efficiency is larger than what expected considering the scintillator thickness of the KLOE prototype only. This result proves the existence of a contribution from the passive material to neutron detection efficiency, in a high-sampling calorimeter configuration.

  17. A suite of standard post-tagging evaluation metrics can help assess tag retention for field-based fish telemetry research

    Science.gov (United States)

    Gerber, Kayla M.; Mather, Martha E.; Smith, Joseph M.

    2017-01-01

    Telemetry can inform many scientific and research questions if a context exists for integrating individual studies into the larger body of literature. Creating cumulative distributions of post-tagging evaluation metrics would allow individual researchers to relate their telemetry data to other studies. Widespread reporting of standard metrics is a precursor to the calculation of benchmarks for these distributions (e.g., mean, SD, 95% CI). Here we illustrate five types of standard post-tagging evaluation metrics using acoustically tagged Blue Catfish (Ictalurus furcatus) released into a Kansas reservoir. These metrics included: (1) percent of tagged fish detected overall, (2) percent of tagged fish detected daily using abacus plot data, (3) average number of (and percent of available) receiver sites visited, (4) date of last movement between receiver sites (and percent of tagged fish moving during that time period), and (5) number (and percent) of fish that egressed through exit gates. These metrics were calculated for one to three time periods: early (of the study (5 months). Over three-quarters of our tagged fish were detected early (85%) and at the end (85%) of the study. Using abacus plot data, all tagged fish (100%) were detected at least one day and 96% were detected for > 5 days early in the study. On average, tagged Blue Catfish visited 9 (50%) and 13 (72%) of 18 within-reservoir receivers early and at the end of the study, respectively. At the end of the study, 73% of all tagged fish were detected moving between receivers. Creating statistical benchmarks for individual metrics can provide useful reference points. In addition, combining multiple metrics can inform ecology and research design. Consequently, individual researchers and the field of telemetry research can benefit from widespread, detailed, and standard reporting of post-tagging detection metrics.

  18. Integrated detection of both 5-mC and 5-hmC by high-throughput tag sequencing technology highlights methylation reprogramming of bivalent genes during cellular differentiation.

    Science.gov (United States)

    Gao, Fei; Xia, Yudong; Wang, Junwen; Luo, Huijuan; Gao, Zhaowei; Han, Xu; Zhang, Juyong; Huang, Xiaojun; Yao, Yu; Lu, Hanlin; Yi, Na; Zhou, Baojin; Lin, Zhilong; Wen, Bo; Zhang, Xiuqing; Yang, Huanming; Wang, Jun

    2013-04-01

    5-methylcytosine (5-mC) can be oxidized to 5-hydroxymethylcytosine (5-hmC). Genome-wide profiling of 5-hmC thus far indicates 5-hmC may not only be an intermediate form of DNA demethylation but could also constitute an epigenetic mark per se. Here we describe a cost-effective and selective method to detect both the hydroxymethylation and methylation status of cytosines in a subset of cytosines in the human genome. This method involves the selective glucosylation of 5-hmC residues, short-Sequence tag generation and high-throughput sequencing. We tested this method by screening H9 human embryonic stem cells and their differentiated embroid body cells, and found that differential hydroxymethylation preferentially occurs in bivalent genes during cellular differentiation. Especially, our results support hydroxymethylation can regulate key transcription regulators with bivalent marks through demethylation and affect cellular decision on choosing active or inactive state of these genes upon cellular differentiation. Future application of this technology would enable us to uncover the status of methylation and hydroxymethylation in dynamic biological processes and disease development in multiple biological samples.

  19. Simplified, enhanced protein purification using an inducible, autoprocessing enzyme tag.

    Directory of Open Access Journals (Sweden)

    Aimee Shen

    2009-12-01

    Full Text Available We introduce a new method for purifying recombinant proteins expressed in bacteria using a highly specific, inducible, self-cleaving protease tag. This tag is comprised of the Vibrio cholerae MARTX toxin cysteine protease domain (CPD, an autoprocessing enzyme that cleaves exclusively after a leucine residue within the target protein-CPD junction. Importantly, V. cholerae CPD is specifically activated by inositol hexakisphosphate (InsP(6, a eukaryotic-specific small molecule that is absent from the bacterial cytosol. As a result, when His(6-tagged CPD is fused to the C-terminus of target proteins and expressed in Escherichia coli, the full-length fusion protein can be purified from bacterial lysates using metal ion affinity chromatography. Subsequent addition of InsP(6 to the immobilized fusion protein induces CPD-mediated cleavage at the target protein-CPD junction, releasing untagged target protein into the supernatant. This method condenses affinity chromatography and fusion tag cleavage into a single step, obviating the need for exogenous protease addition to remove the fusion tag(s and increasing the efficiency of tag separation. Furthermore, in addition to being timesaving, versatile, and inexpensive, our results indicate that the CPD purification system can enhance the expression, integrity, and solubility of intractable proteins from diverse organisms.

  20. Coenzyme- and His-tag-induced crystallization of octopine dehydrogenase

    International Nuclear Information System (INIS)

    Smits, Sander H. J.; Mueller, Andre; Grieshaber, Manfred K.; Schmitt, Lutz

    2008-01-01

    The crystal structure of octopine dehydrogenase revealed a specific role of the His 5 tag in inducing the crystal contacts required for successful crystallization. Over the last decade, protein purification has become more efficient and standardized through the introduction of affinity tags. The choice and position of the tag, however, can directly influence the process of protein crystallization. Octopine dehydrogenase (OcDH) without a His tag and tagged protein constructs such as OcDH-His 5 and OcDH-LEHis 6 have been investigated for their crystallizability. Only OcDH-His 5 yielded crystals; however, they were multiple. To improve crystal quality, the cofactor NADH was added, resulting in single crystals that were suitable for structure determination. As shown by the structure, the His 5 tag protrudes into the cleft between the NADH and l-arginine-binding domains and is mainly fixed in place by water molecules. The protein is thereby stabilized to such an extent that the formation of crystal contacts can proceed. Together with NADH, the His 5 tag obviously locks the enzyme into a specific conformation which induces crystal growth

  1. Early and efficient detection of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures.

    Directory of Open Access Journals (Sweden)

    Benson R Kidenya

    Full Text Available Early, efficient and inexpensive methods for the detection of pulmonary tuberculosis are urgently needed for effective patient management as well as to interrupt transmission. These methods to detect M. tuberculosis in a timely and affordable way are not yet widely available in resource-limited settings. In a developing-country setting, we prospectively evaluated two methods for culturing and detecting M. tuberculosis in sputum. Sputum samples were cultured in liquid assay (micro broth culture in microplate wells and growth was detected by microscopic observation, or in Löwenstein-Jensen (LJ solid media where growth was detected by visual inspection for colonies. Sputum samples were collected from 321 tuberculosis (TB suspects attending Bugando Medical Centre, in Mwanza, Tanzania, and were cultured in parallel. Pulmonary tuberculosis cases were diagnosed using the American Thoracic Society diagnostic standards. There were a total of 200 (62.3% pulmonary tuberculosis cases. Liquid assay with microscopic detection detected a significantly higher proportion of cases than LJ solid culture: 89.0% (95% confidence interval [CI], 84.7% to 93.3% versus 77.0% (95% CI, 71.2% to 82.8% (p = 0.0007. The median turn around time to diagnose tuberculosis was significantly shorter for micro broth culture than for the LJ solid culture, 9 days (interquartile range [IQR] 7-13, versus 21 days (IQR 14-28 (p<0.0001. The cost for micro broth culture (labor inclusive in our study was US $4.56 per sample, versus US $11.35 per sample for the LJ solid culture. The liquid assay (micro broth culture is an early, feasible, and inexpensive method for detection of pulmonary tuberculosis in resource limited settings.

  2. Detection efficiency of the VLF World-Wide Lightning Location Network (WWLLN: initial case study

    Directory of Open Access Journals (Sweden)

    C. J. Rodger

    2006-12-01

    Full Text Available An experimental Very Low Frequency (VLF World-Wide Lightning Location Network (WWLLN has been developed through collaborations with research institutions across the world, providing global real-time locations of lightning discharges. As of April 2006, the network included 25 stations providing coverage for much of the Earth. In this paper we examine the detection efficiency of the WWLLN by comparing the locations from this network with lightning location data purchased from a commercial lightning location network operating in New Zealand. Our analysis confirms that WWLLN favours high peak current return stroke lightning discharges, and that discharges with larger currents are observed by more stations across the global network. We then construct a first principles detection efficiency model to describe the WWLLN, combining calibration information for each station with theoretical modelling to describe the expected amplitudes of the VLF sferics observed by the network. This detection efficiency model allows the prediction of the global variation in WWLLN lightning detection, and an estimate of the minimum CG return stroke peak current required to trigger the network. There are strong spatial variations across the globe, primarily due to station density and sensitivity.

    The WWLLN is currently best suited to study the occurrence and impacts of high peak-current lightning. For example, in 2005 about 12% of the global elve-producing lightning will have been located by the network. Since the lightning-EMP which produce elves has a high mean rate (210 per minute it has the potential to significantly influence the ionosphere on regional scales.

  3. Evaluation of gel electrophoresis conditions for the separation of metal-tagged proteins with subsequent laser ablation ICP-MS detection.

    Science.gov (United States)

    Raab, Andrea; Pioselli, Barbara; Munro, Caroline; Thomas-Oates, Jane; Feldmann, Jörg

    2009-01-01

    Although laser ablation (LA)-ICP-MS has been reported for the determination of metalloproteins separated by gel electrophoretic techniques (GE), systematic studies that define the conditions essential for successful measurements are still scarce. In this paper we present the results of our studies of basic conditions for the effective application of GE-LA-ICP-MS for the separation of metal-binding proteins, focusing on their stability during GE and post-separation gel treatment. The stability of metal-protein complexes (haemoglobin, myoglobin, superoxide dismutase, carbonic anhydrase, transferrin, albumin, cytochrome c) during GE is dependent on the nature of the metal-protein interaction and the principle of separation. We have observed that non-denaturing GE is a suitable separation technique for most metal-protein complexes (e.g. Zn in carbonic anhydrase and Fe in Tf and myoglobin were quantitatively recovered in a spiked liver cytosol), whereas separation by denaturing GE strongly impaired the stability of the complexes. Equally important is the post-separation treatment of the gel to enable successful detection of the metal. LA-ICP-MS requires drying of the gel without loss of protein-bound metal or cracking of the gel. This was successfully achieved using glycerol followed by heating. We demonstrate that staining of the gel prior to LA-ICP-MS using silver or Coomassie blue is not recommended, since most protein-bound metal is lost during the staining procedure. Furthermore it has been shown that only line scanning with a speed of less than 30 microm/s can reliably distinguish between lines 1 mm apart, while raster spot analysis carries the risk of misinterpretation due to contamination in/on inhomogeneous gels.

  4. Addition of Carbon to the Culture Medium Improves the Detection Efficiency of Aflatoxin Synthetic Fungi

    Directory of Open Access Journals (Sweden)

    Tadahiro Suzuki

    2016-11-01

    Full Text Available Aflatoxin (AF is a harmful secondary metabolite that is synthesized by the Aspergillus species. Although AF detection techniques have been developed, techniques for detection of AF synthetic fungi are still required. Techniques such as plate culture methods are continually being modified for this purpose. However, plate culture methods require refinement because they suffer from several issues. In this study, activated charcoal powder (carbon was added to a culture medium containing cyclodextrin (CD to enhance the contrast of fluorescence and improve the detection efficiency for AF synthetic fungi. Two culture media, potato dextrose agar and yeast extract sucrose agar, were investigated using both plate and liquid cultures. The final concentrations of CD and carbon in the media were 3 mg/mL and 0.3 mg/mL, respectively. Addition of carbon improved the visibility of fluorescence by attenuating approximately 30% of light scattering. Several fungi that could not be detected with only CD in the medium were detected with carbon addition. The carbon also facilitated fungal growth in the potato dextrose liquid medium. The results suggest that addition of carbon to media can enhance the observation of AF-derived fluorescence.

  5. The efficiency of concentration methods used to detect enteric viruses in anaerobically digested sludge

    Directory of Open Access Journals (Sweden)

    Tatiana Prado

    2013-02-01

    Full Text Available The presence of enteric viruses in biosolids can be underestimated due to the inefficient methods (mainly molecular methods used to recover the viruses from these matrices. Therefore, the goal of this study was to evaluate the different methods used to recover adenoviruses (AdV, rotavirus species A (RVA, norovirus genogroup II (NoV GII and the hepatitis A virus (HAV from biosolid samples at a large urban wastewater treatment plant in Brazil after they had been treated by mesophilic anaerobic digestion. Quantitative polymerase chain reaction (PCR was used for spiking experiments to compare the detection limits of feasible methods, such as beef extract elution and ultracentrifugation. Tests were performed to detect the inhibition levels and the bacteriophage PP7 was used as an internal control. The results showed that the inhibitors affected the efficiency of the PCR reaction and that beef extract elution is a suitable method for detecting enteric viruses, mainly AdV from biosolid samples. All of the viral groups were detected in the biosolid samples: AdV (90%, RVA, NoV GII (45% and HAV (18%, indicating the viruses' resistance to the anaerobic treatment process. This is the first study in Brazil to detect the presence of RVA, AdV, NoV GII and HAV in anaerobically digested sludge, highlighting the importance of adequate waste management.

  6. A method for real-time memory efficient implementation of blob detection in large images

    Directory of Open Access Journals (Sweden)

    Petrović Vladimir L.

    2017-01-01

    Full Text Available In this paper we propose a method for real-time blob detection in large images with low memory cost. The method is suitable for implementation on the specialized parallel hardware such as multi-core platforms, FPGA and ASIC. It uses parallelism to speed-up the blob detection. The input image is divided into blocks of equal sizes to which the maximally stable extremal regions (MSER blob detector is applied in parallel. We propose the usage of multiresolution analysis for detection of large blobs which are not detected by processing the small blocks. This method can find its place in many applications such as medical imaging, text recognition, as well as video surveillance or wide area motion imagery (WAMI. We explored the possibilities of usage of detected blobs in the feature-based image alignment as well. When large images are processed, our approach is 10 to over 20 times more memory efficient than the state of the art hardware implementation of the MSER.

  7. Modified EAS Tag Used as a Resistive Sensor Platform

    Directory of Open Access Journals (Sweden)

    Henrik Andersson

    2012-11-01

    Full Text Available In this article, a modified design of an RF Radio Frequency Electronic Article Surveillance (EAS tag, used as a sensor platform, is manufactured and characterized. EAS tags are passive devices consisting of a capacitor and coil, tuned to a resonance frequency readable by the detector equipment, in this case 8.2 MHz. They were originally used to detect whether merchandise was being moved through the detection gates at shop exits, in which case an alarm was triggered. If the capacitance is divided in two and a resistive sensor device inserted in between, the resonant Inductor-Capacitor (LC circuit becomes an Inductor-Capacitor-Capacitor-Resistor LCCR circuit and can be used as a sensor tag. A high sensor resistance means that one capacitor is decoupled, leading to one resonance frequency, while a low resistance will couple both capacitances into the circuit, resulting in a lower resonance frequency. Different types of resistive sensors exist that are able to detect properties such as pressure, moisture, light and temperature. The tag is manufactured in Aluminum foil on a polyetylentereftalat (PET substrate, resulting in a cost effective RF-platform for various resistive sensors. Two types of tags are designed and manufactured, one with parallel plate capacitors and the other with interdigital capacitors. To test the tags, a resistive tilt sensor is mounted and the tags are characterized using a network analyzer. It is shown that for high resistance, the tags have a resonance frequency of morethan 10 MHz while for low values the frequency approaches 8.2 MHz.

  8. Spectrally efficient polarization multiplexed direct-detection OFDM system without frequency gap.

    Science.gov (United States)

    Wei, Chia-Chien; Zeng, Wei-Siang; Lin, Chun-Ting

    2016-01-25

    We experimentally demonstrate a spectrally efficient direct-detection orthogonal frequency-division multiplexing (DD-OFDM) system. In addition to polarization-division multiplexing, removing the frequency gap further improves the spectral efficiency of the OFDM system. The frequency gap between a reference carrier and OFDM subcarriers avoids subcarrier-to-subcarrier beating interference (SSBI) in traditional DD-OFDM systems. Without dynamic polarization control, the resulting interference after square-law direct detection in the proposed gap-less system is polarization-dependent and composed of linear inter-carrier interference (ICI) and nonlinear SSBI. Thus, this work proposes an iterative multiple-input multiple-output detection scheme to remove the mixed polarization-dependent interference. Compared to the previous scheme, which only removes ICI, the proposed scheme can further eliminate SSBI to achieve the improvement of ∼ 7 dB in signal-to-noise ratio. Without the need for polarization control, we successfully utilize 7-GHz bandwidth to transmit a 39.5-Gbps polarization multiplexed OFDM signal over 100 km.

  9. Energy Efficient Monitoring for Intrusion Detection in Battery-Powered Wireless Mesh Networks

    KAUST Repository

    Hassanzadeh, Amin

    2011-07-18

    Wireless Mesh Networks (WMN) are easy-to-deploy, low cost solutions for providing networking and internet services in environments with no network infrastructure, e.g., disaster areas and battlefields. Since electric power is not readily available in such environments battery-powered mesh routers, operating in an energy efficient manner, are required. To the best of our knowledge, the impact of energy efficient solutions, e.g., involving duty-cycling, on WMN intrusion detection systems, which require continuous monitoring, remains an open research problem. In this paper we propose that carefully chosen monitoring mesh nodes ensure continuous and complete detection coverage, while allowing non-monitoring mesh nodes to save energy through duty-cycling. We formulate the monitoring node selection problem as an optimization problem and propose distributed and centralized solutions for it, with different tradeoffs. Through extensive simulations and a proof-of-concept hardware/software implementation we demonstrate that our solutions extend the WMN lifetime by 8%, while ensuring, at the minimum, a 97% intrusion detection rate.

  10. Evaluation of the Detection Efficiency of LYSO Scintillator in the Fiber-Optic Radiation Sensor

    Directory of Open Access Journals (Sweden)

    Chan Hee Park

    2014-01-01

    Full Text Available The aim of this study was to develop and evaluate fiber-optic sensors for the remote detection of gamma rays in areas that are difficult to access, such as a spent fuel pool. The fiber-optic sensor consists of a light-generating probe, such as scintillators for radiation detection, plastic optical fibers, and light-measuring devices, such as PMT. The (Lu,Y2SiO5:Ce(LYSO:Ce scintillator was chosen as the light-generating probe. The (Lu,Y2SiO5:Ce(LYSO:Ce scintillator has higher scintillation efficiency than the others and transmits light well through an optical fiber because its refraction index is similar to the refractive index of the optical fiber. The fiber-optic radiation sensor using the (Lu,Y2SiO5:Ce(LYSO:Ce scintillator was evaluated in terms of the detection efficiency and reproducibility for examining its applicability as a radiation sensor.

  11. Advanced Multilayer Composite Heavy-Oxide Scintillator Detectors for High Efficiency Fast Neutron Detection

    Science.gov (United States)

    Ryzhikov, Vladimir D.; Naydenov, Sergei V.; Pochet, Thierry; Onyshchenko, Gennadiy M.; Piven, Leonid A.; Smith, Craig F.

    2018-01-01

    We have developed and evaluated a new approach to fast neutron and neutron-gamma detection based on large-area multilayer composite heterogeneous detection media consisting of dispersed granules of small-crystalline scintillators contained in a transparent organic (plastic) matrix. Layers of the composite material are alternated with layers of transparent plastic scintillator material serving as light guides. The resulting detection medium - designated as ZEBRA - serves as both an active neutron converter and a detection scintillator which is designed to detect both neutrons and gamma-quanta. The composite layers of the ZEBRA detector consist of small heavy-oxide scintillators in the form of granules of crystalline BGO, GSO, ZWO, PWO and other materials. We have produced and tested the ZEBRA detector of sizes 100x100x41 mm and greater, and determined that they have very high efficiency of fast neutron detection (up to 49% or greater), comparable to that which can be achieved by large sized heavy-oxide single crystals of about Ø40x80 cm3 volume. We have also studied the sensitivity variation to fast neutron detection by using different types of multilayer ZEBRA detectors of 100 cm2 surface area and 41 mm thickness (with a detector weight of about 1 kg) and found it to be comparable to the sensitivity of a 3He-detector representing a total cross-section of about 2000 cm2 (with a weight of detector, including its plastic moderator, of about 120 kg). The measured count rate in response to a fast neutron source of 252Cf at 2 m for the ZEBRA-GSO detector of size 100x100x41 mm3 was 2.84 cps/ng, and this count rate can be doubled by increasing the detector height (and area) up to 200x100 mm2. In summary, the ZEBRA detectors represent a new type of high efficiency and low cost solid-state neutron detector that can be used for stationary neutron/gamma portals. They may represent an interesting alternative to expensive, bulky gas counters based on 3He or 10B neutron

  12. A wireless sensor tag platform for container security and integrity

    Science.gov (United States)

    Amaya, Ivan A.; Cree, Johnathan V.; Mauss, Fredrick J.

    2011-04-01

    Cargo containers onboard ships are widely used in the global supply chain. The need for container security is evidenced by the Container Security Initiative launched by the U.S. Bureau of Customs and Border Protection (CBP). One method of monitoring cargo containers is using low power wireless sensor tags. The wireless sensor tags are used to set up a network that is comprised of tags internal to the container and a central device. The sensor network reports alarms and other anomalies to a central device, which then relays the message to an outside network upon arrival at the destination port. This allows the port authorities to have knowledge of potential security or integrity issues before physically examining the container. Challenges of using wireless sensor tag networks for container security include battery life, size, environmental conditions, information security, and cost among others. PNNL developed an active wireless sensor tag platform capable of reporting data wirelessly to a central node as well as logging data to nonvolatile memory. The tags, operate at 2.4 GHz over an IEEE 802.15.4 protocol, and were designed to be distributed throughout the inside of a shipping container in the upper support frame. The tags are mounted in a housing that allows for simple and efficient installation or removal prior to, during, or after shipment. The distributed tags monitor the entire container volume. The sensor tag platform utilizes low power electronics and provides an extensible sensor interface for incorporating a wide range of sensors including chemical, biological, and environmental sensors.

  13. Efficient, Decentralized Detection of Qualitative Spatial Events in a Dynamic Scalar Field

    Directory of Open Access Journals (Sweden)

    Myeong-Hun Jeong

    2015-08-01

    Full Text Available This paper describes an efficient, decentralized algorithm to monitor qualitative spatial events in a dynamic scalar field. The events of interest involve changes to the critical points (i.e., peak, pits and passes and edges of the surface network derived from the field. Four fundamental types of event (appearance, disappearance, movement and switch are defined. Our algorithm is designed to rely purely on qualitative information about the neighborhoods of nodes in the sensor network and does not require information about nodes’ coordinate positions. Experimental investigations confirm that our algorithm is efficient, with O(n overall communication complexity (where n is the number of nodes in the sensor network, an even load balance and low operational latency. The accuracy of event detection is comparable to established centralized algorithms for the identification of critical points of a surface network. Our algorithm is relevant to a broad range of environmental monitoring applications of sensor networks.

  14. An efficient sampling algorithm for uncertain abnormal data detection in biomedical image processing and disease prediction.

    Science.gov (United States)

    Liu, Fei; Zhang, Xi; Jia, Yan

    2015-01-01

    In this paper, we propose a computer information processing algorithm that can be used for biomedical image processing and disease prediction. A biomedical image is considered a data object in a multi-dimensional space. Each dimension is a feature that can be used for disease diagnosis. We introduce a new concept of the top (k1,k2) outlier. It can be used to detect abnormal data objects in the multi-dimensional space. This technique focuses on uncertain space, where each data object has several possible instances with distinct probabilities. We design an efficient sampling algorithm for the top (k1,k2) outlier in uncertain space. Some improvement techniques are used for acceleration. Experiments show our methods' high accuracy and high efficiency.

  15. Quantum tagging for tags containing secret classical data

    International Nuclear Information System (INIS)

    Kent, Adrian

    2011-01-01

    Various authors have considered schemes for quantum tagging, that is, authenticating the classical location of a classical tagging device by sending and receiving quantum signals from suitably located distant sites, in an environment controlled by an adversary whose quantum information processing and transmitting power is potentially unbounded. All of the schemes proposed elsewhere in the literature assume that the adversary is able to inspect the interior of the tagging device. All of these schemes have been shown to be breakable if the adversary has unbounded predistributed entanglement. We consider here the case in which the tagging device contains a finite key string shared with distant sites but kept secret from the adversary, and show this allows the location of the tagging device to be authenticated securely and indefinitely. Our protocol relies on quantum key distribution between the tagging device and at least one distant site, and demonstrates a new practical application of quantum key distribution. It also illustrates that the attainable security in position-based cryptography can depend crucially on apparently subtle details in the security scenario considered.

  16. TOWARDS A MORE EFFICIENT DETECTION OF EARTHQUAKE INDUCED FAÇADE DAMAGES USING OBLIQUE UAV IMAGERY

    Directory of Open Access Journals (Sweden)

    D. Duarte

    2017-08-01

    Full Text Available Urban search and rescue (USaR teams require a fast and thorough building damage assessment, to focus their rescue efforts accordingly. Unmanned aerial vehicles (UAV are able to capture relevant data in a short time frame and survey otherwise inaccessible areas after a disaster, and have thus been identified as useful when coupled with RGB cameras for façade damage detection. Existing literature focuses on the extraction of 3D and/or image features as cues for damage. However, little attention has been given to the efficiency of the proposed methods which hinders its use in an urban search and rescue context. The framework proposed in this paper aims at a more efficient façade damage detection using UAV multi-view imagery. This was achieved directing all damage classification computations only to the image regions containing the façades, hence discarding the irrelevant areas of the acquired images and consequently reducing the time needed for such task. To accomplish this, a three-step approach is proposed: i building extraction from the sparse point cloud computed from the nadir images collected in an initial flight; ii use of the latter as proxy for façade location in the oblique images captured in subsequent flights, and iii selection of the façade image regions to be fed to a damage classification routine. The results show that the proposed framework successfully reduces the extracted façade image regions to be assessed for damage 6 fold, hence increasing the efficiency of subsequent damage detection routines. The framework was tested on a set of UAV multi-view images over a neighborhood of the city of L’Aquila, Italy, affected in 2009 by an earthquake.

  17. Towards a More Efficient Detection of Earthquake Induced FAÇADE Damages Using Oblique Uav Imagery

    Science.gov (United States)

    Duarte, D.; Nex, F.; Kerle, N.; Vosselman, G.

    2017-08-01

    Urban search and rescue (USaR) teams require a fast and thorough building damage assessment, to focus their rescue efforts accordingly. Unmanned aerial vehicles (UAV) are able to capture relevant data in a short time frame and survey otherwise inaccessible areas after a disaster, and have thus been identified as useful when coupled with RGB cameras for façade damage detection. Existing literature focuses on the extraction of 3D and/or image features as cues for damage. However, little attention has been given to the efficiency of the proposed methods which hinders its use in an urban search and rescue context. The framework proposed in this paper aims at a more efficient façade damage detection using UAV multi-view imagery. This was achieved directing all damage classification computations only to the image regions containing the façades, hence discarding the irrelevant areas of the acquired images and consequently reducing the time needed for such task. To accomplish this, a three-step approach is proposed: i) building extraction from the sparse point cloud computed from the nadir images collected in an initial flight; ii) use of the latter as proxy for façade location in the oblique images captured in subsequent flights, and iii) selection of the façade image regions to be fed to a damage classification routine. The results show that the proposed framework successfully reduces the extracted façade image regions to be assessed for damage 6 fold, hence increasing the efficiency of subsequent damage detection routines. The framework was tested on a set of UAV multi-view images over a neighborhood of the city of L'Aquila, Italy, affected in 2009 by an earthquake.

  18. Signal Processing and Its Effect on Scanning Efficiencies for a Field Instrument for Detecting Low-energy Radiation.

    Science.gov (United States)

    Marianno, Craig M

    2015-07-01

    Signal processing within a radiation detector affects detection efficiency. Currently, organizations such as private industry, the U.S. Navy, Army, and Air Force are coupling some detector systems with data collection devices to survey large land areas for radioactive contamination. As detector technology has advanced and analog data collection has turned to digital, signal processing is becoming prevalent in some instruments. Using a NIST traceable (241)Am source, detection efficiency for a field instrument for detecting low-energy radiation (FIDLER) was examined for both a static and scanning mode. Experimental results were compared to Monte Carlo-generated efficiencies. Stationary data compared nicely to the theoretical results. Conversely, scanning detection efficiencies were considerably different from their theoretical counterparts. As speed increased, differences in detection efficiency approached two orders of magnitude. To account for these differences, a quasi time-dependent Monte Carlo simulation was created mimicking the signal processing undertaken by the FIDLER detection system. By including signal processing, experimental results fell within the bounds of the Monte Carlo-generated efficiencies, thus demonstrating the negative effects of such processing on detection efficiencies.

  19. Ultramild protein-mediated click chemistry creates efficient oligonucleotide probes for targeting and detecting nucleic acids

    DEFF Research Database (Denmark)

    Nåbo, Lina J.; Madsen, Charlotte S.; Jensen, Knud J.

    2015-01-01

    Functionalized synthetic oligonucleotides are finding growing applications in research, clinical studies, and therapy. However, it is not easy to prepare them in a biocompatible and highly efficient manner. We report a new strategy to synthesize oligonucleotides with promising nucleic acid...... targeting and detection properties. We focus in particular on the pH sensitivity of these new probes and their high target specificity. For the first time, human copper(I)-binding chaperon Cox17 was applied to effectively catalyze click labeling of oligonucleotides. This was performed under ultramild...

  20. A spectrally efficient detect-and-forward scheme with two-tier adaptive cooperation

    KAUST Repository

    Benjillali, Mustapha

    2011-09-01

    We propose a simple relay-based adaptive cooperation scheme to improve the spectral efficiency of "Detect-and-Forward" (DetF) half-duplex relaying in fading channels. In a new common framework, we show that the proposed scheme offers considerable gainsin terms of the achievable information ratescompared to conventional DetF relaying schemes for both orthogonal and non-orthogonal source/relay transmissions. The analysis leads on to a general adaptive cooperation strategy based on the maximization of information rates at the destination which needs to observe only the average signal-to-noise ratios of the links. © 2006 IEEE.

  1. Single photon timing resolution and detection efficiency of the IRST silicon photo-multipliers

    International Nuclear Information System (INIS)

    Collazuol, G.; Ambrosi, G.; Boscardin, M.; Corsi, F.; Dalla Betta, G.F.; Del Guerra, A.; Dinu, N.; Galimberti, M.; Giulietti, D.; Gizzi, L.A.; Labate, L.; Llosa, G.; Marcatili, S.; Morsani, F.; Piemonte, C.; Pozza, A.; Zaccarelli, L.; Zorzi, N.

    2007-01-01

    Silicon photo-multipliers (SiPM) consist in matrices of tiny, passive quenched avalanche photo-diode cells connected in parallel via integrated resistors and operated in Geiger mode. Novel types of SiPM are being developed at FBK-IRST (Trento, Italy). Despite their classical shallow junction n-on-p structure the devices are unique in their enhanced photo-detection efficiency (PDE) for short-wavelengths and in their low level of dark rate and excess noise factor. After a summary of the extensive SiPM characterization we will focus on the study of PDE and the single photon timing resolution

  2. Validity and efficiency of conformal anomaly detection on big distributed data

    Directory of Open Access Journals (Sweden)

    Ilia Nouretdinov

    2017-05-01

    Full Text Available Conformal Prediction is a recently developed framework for reliable confident predictions. In this work we discuss its possible application to big data coming from different, possibly heterogeneous data sources. On example of anomaly detection problem, we study the question of saving validity of Conformal Prediction in this case. We show that the straight forward averaging approach is invalid, while its easy alternative of maximizing is not very efficient because of its conservativeness. We propose the third compromised approach that is valid, but much less conservative. It is supported by both theoretical justification and experimental results in the area of energy engineering.

  3. Efficient estimation of dynamic density functions with an application to outlier detection

    KAUST Repository

    Qahtan, Abdulhakim Ali Ali

    2012-01-01

    In this paper, we propose a new method to estimate the dynamic density over data streams, named KDE-Track as it is based on a conventional and widely used Kernel Density Estimation (KDE) method. KDE-Track can efficiently estimate the density with linear complexity by using interpolation on a kernel model, which is incrementally updated upon the arrival of streaming data. Both theoretical analysis and experimental validation show that KDE-Track outperforms traditional KDE and a baseline method Cluster-Kernels on estimation accuracy of the complex density structures in data streams, computing time and memory usage. KDE-Track is also demonstrated on timely catching the dynamic density of synthetic and real-world data. In addition, KDE-Track is used to accurately detect outliers in sensor data and compared with two existing methods developed for detecting outliers and cleaning sensor data. © 2012 ACM.

  4. Fast Temporal Activity Proposals for Efficient Detection of Human Actions in Untrimmed Videos

    KAUST Repository

    Heilbron, Fabian Caba

    2016-12-13

    In many large-scale video analysis scenarios, one is interested in localizing and recognizing human activities that occur in short temporal intervals within long untrimmed videos. Current approaches for activity detection still struggle to handle large-scale video collections and the task remains relatively unexplored. This is in part due to the computational complexity of current action recognition approaches and the lack of a method that proposes fewer intervals in the video, where activity processing can be focused. In this paper, we introduce a proposal method that aims to recover temporal segments containing actions in untrimmed videos. Building on techniques for learning sparse dictionaries, we introduce a learning framework to represent and retrieve activity proposals. We demonstrate the capabilities of our method in not only producing high quality proposals but also in its efficiency. Finally, we show the positive impact our method has on recognition performance when it is used for action detection, while running at 10FPS.

  5. A General Method for Intracellular Protein Delivery through 'E-tag' Protein Engineering and Arginine Functionalized Gold Nanoparticles.

    Science.gov (United States)

    Mout, Rubul; Rotello, Vincent M

    2017-12-20

    In this protocol, we describe a method for direct cytosolic protein delivery that avoids endosomal entrapment of the delivered proteins. We achieved this by tagging the desired protein with an oligo glutamic acid tag (E-tag), and subsequently using carrier gold nanoparticles to deliver these E-tagged proteins. When E-tagged proteins and nanoparticles were mixed, they formed nanoassemblies, which got fused to cell membrane upon incubation and directly released the E-tagged protein into cell cytosol. We used this method to deliver a wide variety of proteins with different sizes, charges, and functions in various cell lines (Mout et al ., 2017). To use this protocol, the first step is to generate the required materials (gold nanoparticles, recombinant E-tagged proteins). Laboratory-synthesis of gold nanoparticles has been previously described (Yang et al ., 2011). Desired E-tagged proteins can be cloned from the corresponding genes, and expressed and purified using standard laboratory procedures. We will use E-tagged green fluorescent protein (GFP) as a reference protein here. Users can simply insert an E-tag into their protein of interest, at either terminus. To achieve maximum delivery efficiency, we suggest users testing different length of E-tags. For example, we inserted E = 0 to 20 (E0 means no E-tag insertion, and E20 means 20 glutamic acids insertion in a row) to most of the proteins we tested, and screened for optimal E-tagged length for highest delivery efficiency. E10-tagged proteins gave us the highest delivery efficiency for most of the proteins (except for Cas9, where E20 tag showed highest delivery efficiency). Once these materials are ready, it takes about ~10 min to make the E-tagged protein and nanoparticle nanoassemblies, which are immediately used for delivery. Complete delivery (~100% for GFP-E10) is achieved in less than 3 h.

  6. Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity.

    Science.gov (United States)

    Aviv, Gili; Gal-Mor, Ohad

    2018-01-01

    Western blotting is a common approach to detect the presence of a target protein in biological samples or proteins mixture using specific antibodies. This method is also useful to study regulation of virulence determinants by analyzing changes in protein expression between different genetic backgrounds or under varying environmental conditions. To avoid the need to raise specific antibodies for each studied protein, commercial antibody against commonly used peptidic epitopes can be utilized if the right target tagged version is constructed. Here we describe a C-terminal fusion between a protein of interest and the two hemagglutinin A (2HA) tag. The tagged protein is cloned into a low-copy number vector and expressed under its native promoter in Salmonella enterica. Then, the expression of the tagged protein can be analyzed by Western blotting and commercially available anti-2HA antibodies.

  7. Efficiency of GC-MS method in detection of beeswax adulterated with paraffin

    Directory of Open Access Journals (Sweden)

    Waś Ewa

    2016-06-01

    Full Text Available The efficiency of the gas chromatography - mass detector (GC-MS technique for the detection of beeswax adulterated with paraffin, was evaluated. For this purpose, beeswax samples with paraffin additions (3, 5, 10, 30, 50% were analysed. Since not enough is known about paraffin compositions, and since it is difficult to detect paraffin in beeswax, the aim of our research was also to compare the hydrocarbon composition of different types of paraffin. The analysis showed that the types of paraffin available on the market, differ qualitatively and quantitatively as far as their hydrocarbon compositions are concerned. In all kinds of paraffin, we found homologous series of n-alkanes that were much longer than those in beeswax. In beeswax, the amount of added paraffin that is possible to detect, differs and depends on the kind of paraffin used for adulteration. In this study, the minimum estimated percent that was detected using the GC-MS technique, was 3%. The adulteration is indicated by the presence of hydrocarbons containing over 35 carbon atoms in the molecule, and by the higher contents of n-alkanes (C20H42 - C35H72, in comparison to the concentration of these compounds determined in pure beeswax. We also presented the results of the quality control of commercial beeswax. Based on our results, it can be stated that beeswax adulteration is currently a problem.

  8. An Energy-Efficient Multi-Tier Architecture for Fall Detection Using Smartphones.

    Science.gov (United States)

    Guvensan, M Amac; Kansiz, A Oguz; Camgoz, N Cihan; Turkmen, H Irem; Yavuz, A Gokhan; Karsligil, M Elif

    2017-06-23

    Automatic detection of fall events is vital to providing fast medical assistance to the causality, particularly when the injury causes loss of consciousness. Optimization of the energy consumption of mobile applications, especially those which run 24/7 in the background, is essential for longer use of smartphones. In order to improve energy-efficiency without compromising on the fall detection performance, we propose a novel 3-tier architecture that combines simple thresholding methods with machine learning algorithms. The proposed method is implemented on a mobile application, called uSurvive, for Android smartphones. It runs as a background service and monitors the activities of a person in daily life and automatically sends a notification to the appropriate authorities and/or user defined contacts when it detects a fall. The performance of the proposed method was evaluated in terms of fall detection performance and energy consumption. Real life performance tests conducted on two different models of smartphone demonstrate that our 3-tier architecture with feature reduction could save up to 62% of energy compared to machine learning only solutions. In addition to this energy saving, the hybrid method has a 93% of accuracy, which is superior to thresholding methods and better than machine learning only solutions.

  9. An Energy Efficient ECG Signal Processor Detecting Cardiovascular Diseases on Smartphone.

    Science.gov (United States)

    Jain, Sanjeev Kumar; Bhaumik, Basabi

    2017-04-01

    A novel disease diagnostic algorithm for ECG signal processing based on forward search is implemented in Application Specific Integrated Circuit (ASIC) for cardiovascular disease diagnosis on smartphone. An ASIC is fabricated using 130-nm CMOS low leakage process technology. The area of our PQRST ASIC is 1.21 mm 2 . The energy dissipation of PQRST ASIC is 96 pJ with a supply voltage of 0.9 V. The outputs from the ASIC are fed to an Android application that generates diagnostic report and can be sent to a cardiologist via email. The ASIC and Android application are verified for the detection of bundle branch block, hypertrophy, arrhythmia and myocardial infarction using Physionet PTB diagnostic ECG database. The failed detection rate is 0.69%, 0.69%, 0.34% and 1.72% for bundle branch block, hypertrophy, arrhythmia and myocardial infarction respectively. The AV block is detected in all the three patients in the Physionet St. Petersburg arrhythmia database. Our proposed ASIC together with our Android application is the most suitable for an energy efficient wearable cardiovascular disease detection system.

  10. Design of an HF-Band RFID System with Multiple Readers and Passive Tags for Indoor Mobile Robot Self-Localization.

    Science.gov (United States)

    Mi, Jian; Takahashi, Yasutake

    2016-07-29

    Radio frequency identification (RFID) technology has already been explored for efficient self-localization of indoor mobile robots. A mobile robot equipped with RFID readers detects passive RFID tags installed on the floor in order to locate itself. The Monte-Carlo localization (MCL) method enables the localization of a mobile robot equipped with an RFID system with reasonable accuracy, sufficient robustness and low computational cost. The arrangements of RFID readers and tags and the size of antennas are important design parameters for realizing accurate and robust self-localization using a low-cost RFID system. The design of a likelihood model of RFID tag detection is also crucial for the accurate self-localization. This paper presents a novel design and arrangement of RFID readers and tags for indoor mobile robot self-localization. First, by considering small-sized and large-sized antennas of an RFID reader, we show how the design of the likelihood model affects the accuracy of self-localization. We also design a novel likelihood model by taking into consideration the characteristics of the communication range of an RFID system with a large antenna. Second, we propose a novel arrangement of RFID tags with eight RFID readers, which results in the RFID system configuration requiring much fewer readers and tags while retaining reasonable accuracy of self-localization. We verify the performances of MCL-based self-localization realized using the high-frequency (HF)-band RFID system with eight RFID readers and a lower density of RFID tags installed on the floor based on MCL in simulated and real environments. The results of simulations and real environment experiments demonstrate that our proposed low-cost HF-band RFID system realizes accurate and robust self-localization of an indoor mobile robot.

  11. An Design of HF-Band RFID System with Multiple Readers and Passive Tags for Indoor Mobile Robot Self-Localization

    Directory of Open Access Journals (Sweden)

    Jian Mi

    2016-07-01

    Full Text Available Radio frequency identification (RFID technology has already been explored for efficient self-localization of indoor mobile robots. A mobile robot equipped with RFID readers detects passive RFID tags installed on the floor in order to locate itself. The Monte-Carlo localization (MCL method enables the localization of a mobile robot equipped with an RFID system with reasonable accuracy, sufficient robustness and low computational cost. The arrangements of RFID readers and tags and the size of antennas are important design parameters for realizing accurate and robust self-localization using a low-cost RFID system. The design of a likelihood model of RFID tag detection is also crucial for the accurate self-localization. This paper presents a novel design and arrangement of RFID readers and tags for indoor mobile robot self-localization. First, by considering small-sized and large-sized antennas of an RFID reader, we show how the design of the likelihood model affects the accuracy of self-localization. We also design a novel likelihood model by taking into consideration the characteristics of the communication range of an RFID system with a large antenna. Second, we propose a novel arrangement of RFID tags with eight RFID readers, which results in the RFID system configuration requiring much fewer readers and tags while retaining reasonable accuracy of self-localization. We verify the performances of MCL-based self-localization realized using the high-frequency (HF-band RFID system with eight RFID readers and a lower density of RFID tags installed on the floor based on MCL in simulated and real environments. The results of simulations and real environment experiments demonstrate that our proposed low-cost HF-band RFID system realizes accurate and robust self-localization of an indoor mobile robot.

  12. Go Pink! The Effect of Secondary Quanta on Detective Quantum Efficiency

    Energy Technology Data Exchange (ETDEWEB)

    Watson, Scott [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-09-05

    Photons are never directly observable. Consequently, we often use photoelectric detectors (eg CCDs) to record associated photoelectrons statistically. Nonetheless, it is an implicit goal of radiographic detector designers to achieve the maximum possible detector efficiency1. In part the desire for ever higher efficiency has been due to the fact that detectors are far less expensive than associated accelerator facilities (e.g. DARHT and PHERMEX2). In addition, higher efficiency detectors often have better spatial resolution. Consequently, the optimization of the detector, not the accelerator, is the system component with the highest leverage per dollar. In recent years, imaging scientists have adopted the so-called Detective Quantum Efficiency, or DQE as a summary measure of detector performance. Unfortunately, owing to the complex nature of the trade-space associated with detector components, and the natural desire for simplicity and low(er) cost, there has been a recent trend in Los Alamos to focus only on the zerofrequency efficiency, or DQE(0), when designing such systems. This narrow focus leads to system designs that neglect or even ignore the importance of high-spatial-frequency image components. In this paper we demonstrate the significant negative impact of these design choices on the Noise Power Spectrum1 (NPS) and recommend a more holistic approach to detector design. Here we present a statistical argument which indicates that a very large number (>20) of secondary quanta (typically visible light and/or recorded photo-electrons) are needed to take maximum advantage of the primary quanta (typically x-rays or protons) which are available to form an image. Since secondary particles come in bursts, they are not independent. In short, we want to maximize the pink nature of detector noise at DARHT.

  13. Large-area NbN superconducting nanowire avalanche photon detectors with saturated detection efficiency

    Science.gov (United States)

    Murphy, Ryan P.; Grein, Matthew E.; Gudmundsen, Theodore J.; McCaughan, Adam; Najafi, Faraz; Berggren, Karl K.; Marsili, Francesco; Dauler, Eric A.

    2015-05-01

    Superconducting circuits comprising SNSPDs placed in parallel—superconducting nanowire avalanche photodetectors, or SNAPs—have previously been demonstrated to improve the output signal-to-noise ratio (SNR) by increasing the critical current. In this work, we employ a 2-SNAP superconducting circuit with narrow (40 nm) niobium nitride (NbN) nanowires to improve the system detection efficiency to near-IR photons while maintaining high SNR. Additionally, while previous 2-SNAP demonstrations have added external choke inductance to stabilize the avalanching photocurrent, we show that the external inductance can be entirely folded into the active area by cascading 2-SNAP devices in series to produce a greatly increased active area. We fabricated series-2-SNAP (s2-SNAP) circuits with a nanowire length of 20 μm with cascades of 2-SNAPs providing the choke inductance necessary for SNAP operation. We observed that (1) the detection efficiency saturated at high bias currents, and (2) the 40 nm 2-SNAP circuit critical current was approximately twice that for a 40 nm non-SNAP configuration.

  14. An Energy-Efficient Cluster-Based Vehicle Detection on Road Network Using Intention Numeration Method

    Directory of Open Access Journals (Sweden)

    Deepa Devasenapathy

    2015-01-01

    Full Text Available The traffic in the road network is progressively increasing at a greater extent. Good knowledge of network traffic can minimize congestions using information pertaining to road network obtained with the aid of communal callers, pavement detectors, and so on. Using these methods, low featured information is generated with respect to the user in the road network. Although the existing schemes obtain urban traffic information, they fail to calculate the energy drain rate of nodes and to locate equilibrium between the overhead and quality of the routing protocol that renders a great challenge. Thus, an energy-efficient cluster-based vehicle detection in road network using the intention numeration method (CVDRN-IN is developed. Initially, sensor nodes that detect a vehicle are grouped into separate clusters. Further, we approximate the strength of the node drain rate for a cluster using polynomial regression function. In addition, the total node energy is estimated by taking the integral over the area. Finally, enhanced data aggregation is performed to reduce the amount of data transmission using digital signature tree. The experimental performance is evaluated with Dodgers loop sensor data set from UCI repository and the performance evaluation outperforms existing work on energy consumption, clustering efficiency, and node drain rate.

  15. Near-Duplicate Web Page Detection: An Efficient Approach Using Clustering, Sentence Feature and Fingerprinting

    Directory of Open Access Journals (Sweden)

    J. Prasanna Kumar

    2013-02-01

    Full Text Available Duplicate and near-duplicate web pages are the chief concerns for web search engines. In reality, they incur enormous space to store the indexes, ultimately slowing down and increasing the cost of serving results. A variety of techniques have been developed to identify pairs of web pages that are aldquo;similarardquo; to each other. The problem of finding near-duplicate web pages has been a subject of research in the database and web-search communities for some years. In order to identify the near duplicate web pages, we make use of sentence level features along with fingerprinting method. When a large number of web documents are in consideration for the detection of web pages, then at first, we use K-mode clustering and subsequently sentence feature and fingerprint comparison is used. Using these steps, we exactly identify the near duplicate web pages in an efficient manner. The experimentation is carried out on the web page collections and the results ensured the efficiency of the proposed approach in detecting the near duplicate web pages.

  16. An Efficient Forensic Method for Copy–move Forgery Detection based on DWT-FWHT

    Directory of Open Access Journals (Sweden)

    B. Yang

    2013-12-01

    Full Text Available As the increased availability of sophisticated image processing software and the widespread use of Internet, digital images are easy to acquire and manipulate. The authenticity of the received images is becoming more and more important. Copy-move forgery is one of the most common forgery methods. When creating a Copy-move forgery, it is often necessary to add or remove important features from an image. To carry out such forensic analysis, various technological instruments have been developed in the literatures. However, most of them are time-consuming. In this paper, a more efficient method is proposed. First, the image size is reduced by Discrete Wavelet Transform (DWT. Second, the image is divided into overlapping blocks of equal size and, feature of each block is extracted by fast Walsh-Hadamard Transform (FWHT. Duplicated regions are then detected by lexicographically sorting all features of the image blocks. To make the range matching more efficient, multi-hop jump (MHJ algorithm is using to jump over some the “unnecessary testing blocks” (UTB. Experimental results demonstrated that the proposed method not only is able to detect the copy-move forgery accurately but also can reduce the processing time greatly compared with other methods.

  17. The influence of aerosol particle collection mechanisms on the WIPP alpha-6 CAM detection efficiency

    International Nuclear Information System (INIS)

    Bartlett, W.T.

    1996-01-01

    Alpha-6 continuous air monitors (CAMs) are used at the Waste Isolation Pilot Plant (WIPP) in the underground salt mine proposed for transuranic waste disposal near Carlsbad, New Mexico. Previous reviews of CAM operational data indicate that alpha spectra, background subtract methods, and alpha detection efficiency are significantly affected by salt aerosol. Gravimetric analyses of CAM sampling filters indicate that sampling-filter salt deposits are of sufficient magnitude to cause spectral degradation and efficiency losses. It was previously assumed that salt aerosol was mechanically collected on the surface of the sampling filter, but other aerosol collection mechanisms, such as electrostatic, diffusional, and inertial impaction, cannot be ruled out. Microscopic observations of the sampling filters indicate that particle form complex structures on the sampling filter surface, and that electrostatic, diffusional, and inertial impaction are occurring. Aerosol particles are likely to penetrate the complex surface salt matrix, and alpha particle energy will be lost before reaching the CAM detector. Penetration of a polydisperse aerosol into the sampling-filter salt deposits accounts for degraded spectra and efficiency loss observed at the WIPP. It was recommended that CAMs should not be considered operational when 0.5 to 2.0 mg cm -2 of sampling-filter salt is present on the sampling filter

  18. Time properties of MWPC using electronegative gases, and their efficiency of detection for normal and abnormal particles (quarks)

    CERN Document Server

    Breidenbach, M; Tirler, R

    1973-01-01

    The efficiency for detection of fractionally charged particles of multiwire proportional chambers using electronegative gases has been investigated, using a model to simulate the ion production and drift in a MWPC and comparing the results with experimental data. (10 refs).

  19. Editorial Tag Endogeneity for News Websites

    OpenAIRE

    Bruno Ribeiro; Ricardo Morla; Amílcar Correia

    2013-01-01

    Editors and journalists at some news websites label their articles with structure and content-related editorial tags. Each article can have more than one tag and each tag can be used in more than one article. A network of tags can be defined whose edges are all possible pairs of tags in each article. Because editorial tags relate to structure and content rather than individual articles, the analysis of a network of editorial tags could assist editorial decisions to prioritize types of content...

  20. Playing tag with ANN: boosted top identification with pattern recognition

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Leandro G. [Institut de Biologie de l’École Normale Supérieure (IBENS), Inserm 1024- CNRS 8197,46 rue d’Ulm, 75005 Paris (France); Backović, Mihailo [Center for Cosmology, Particle Physics and Phenomenology - CP3,Universite Catholique de Louvain,Louvain-la-neuve (Belgium); Cliche, Mathieu [Laboratory for Elementary Particle Physics, Cornell University,Ithaca, NY 14853 (United States); Lee, Seung J. [Department of Physics, Korea Advanced Institute of Science and Technology,335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Korea, Republic of); School of Physics, Korea Institute for Advanced Study,Seoul 130-722 (Korea, Republic of); Perelstein, Maxim [Laboratory for Elementary Particle Physics, Cornell University,Ithaca, NY 14853 (United States)

    2015-07-17

    Many searches for physics beyond the Standard Model at the Large Hadron Collider (LHC) rely on top tagging algorithms, which discriminate between boosted hadronic top quarks and the much more common jets initiated by light quarks and gluons. We note that the hadronic calorimeter (HCAL) effectively takes a “digital image' of each jet, with pixel intensities given by energy deposits in individual HCAL cells. Viewed in this way, top tagging becomes a canonical pattern recognition problem. With this motivation, we present a novel top tagging algorithm based on an Artificial Neural Network (ANN), one of the most popular approaches to pattern recognition. The ANN is trained on a large sample of boosted tops and light quark/gluon jets, and is then applied to independent test samples. The ANN tagger demonstrated excellent performance in a Monte Carlo study: for example, for jets with p{sub T} in the 1100–1200 GeV range, 60% top-tag efficiency can be achieved with a 4% mis-tag rate. We discuss the physical features of the jets identified by the ANN tagger as the most important for classification, as well as correlations between the ANN tagger and some of the familiar top-tagging observables and algorithms.

  1. Playing tag with ANN: boosted top identification with pattern recognition

    International Nuclear Information System (INIS)

    Almeida, Leandro G.; Backović, Mihailo; Cliche, Mathieu; Lee, Seung J.; Perelstein, Maxim

    2015-01-01

    Many searches for physics beyond the Standard Model at the Large Hadron Collider (LHC) rely on top tagging algorithms, which discriminate between boosted hadronic top quarks and the much more common jets initiated by light quarks and gluons. We note that the hadronic calorimeter (HCAL) effectively takes a “digital image" of each jet, with pixel intensities given by energy deposits in individual HCAL cells. Viewed in this way, top tagging becomes a canonical pattern recognition problem. With this motivation, we present a novel top tagging algorithm based on an Artificial Neural Network (ANN), one of the most popular approaches to pattern recognition. The ANN is trained on a large sample of boosted tops and light quark/gluon jets, and is then applied to independent test samples. The ANN tagger demonstrated excellent performance in a Monte Carlo study: for example, for jets with p T in the 1100–1200 GeV range, 60% top-tag efficiency can be achieved with a 4% mis-tag rate. We discuss the physical features of the jets identified by the ANN tagger as the most important for classification, as well as correlations between the ANN tagger and some of the familiar top-tagging observables and algorithms.

  2. Tissue-specific tagging of endogenous loci in Drosophila melanogaster

    Directory of Open Access Journals (Sweden)

    Kate Koles

    2016-01-01

    Full Text Available Fluorescent protein tags have revolutionized cell and developmental biology, and in combination with binary expression systems they enable diverse tissue-specific studies of protein function. However these binary expression systems often do not recapitulate endogenous protein expression levels, localization, binding partners and/or developmental windows of gene expression. To address these limitations, we have developed a method called T-STEP (tissue-specific tagging of endogenous proteins that allows endogenous loci to be tagged in a tissue specific manner. T-STEP uses a combination of efficient CRISPR/Cas9-enhanced gene targeting and tissue-specific recombinase-mediated tag swapping to temporally and spatially label endogenous proteins. We have employed this method to GFP tag OCRL (a phosphoinositide-5-phosphatase in the endocytic pathway and Vps35 (a Parkinson's disease-implicated component of the endosomal retromer complex in diverse Drosophila tissues including neurons, glia, muscles and hemocytes. Selective tagging of endogenous proteins allows, for the first time, cell type-specific live imaging and proteomics in complex tissues.

  3. Adaptive Energy-Efficient Target Detection Based on Mobile Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Tengyue Zou

    2017-05-01

    Full Text Available Target detection is a widely used application for area surveillance, elder care, and fire alarms; its purpose is to find a particular object or event in a region of interest. Usually, fixed observing stations or static sensor nodes are arranged uniformly in the field. However, each part of the field has a different probability of being intruded upon; if an object suddenly enters an area with few guardian devices, a loss of detection will occur, and the stations in the safe areas will waste their energy for a long time without any discovery. Thus, mobile wireless sensor networks may benefit from adaptation and pertinence in detection. Sensor nodes equipped with wheels are able to move towards the risk area via an adaptive learning procedure based on Bayesian networks. Furthermore, a clustering algorithm based on k-means++ and an energy control mechanism is used to reduce the energy consumption of nodes. The extended Kalman filter and a voting data fusion method are employed to raise the localization accuracy of the target. The simulation and experimental results indicate that this new system with adaptive energy-efficient methods is able to achieve better performance than the traditional ones.

  4. An efficient unsupervised fetal QRS complex detection from abdominal maternal ECG.

    Science.gov (United States)

    Varanini, M; Tartarisco, G; Billeci, L; Macerata, A; Pioggia, G; Balocchi, R

    2014-08-01

    Non-invasive fetal heart rate is of great relevance in clinical practice to monitor fetal health state during pregnancy. To date, however, despite significant advances in the field of electrocardiography, the analysis of abdominal fetal ECG is considered a challenging problem for biomedical and signal processing communities. This is mainly due to the low signal-to-noise ratio of fetal ECG and difficulties in cancellation of maternal QRS complexes, motion and electromyographic artefacts. In this paper we present an efficient unsupervised algorithm for fetal QRS complex detection from abdominal multichannel signal recordings combining ICA and maternal ECG cancelling, which outperforms each single method. The signal is first pre-processed to remove impulsive artefacts, baseline wandering and power line interference. The following steps are then applied: maternal ECG extraction through independent component analysis (ICA); maternal QRS detection; maternal ECG cancelling through weighted singular value decomposition; enhancing of fetal ECG through ICA and fetal QRS detection. We participated in the Physionet/Computing in Cardiology Challenge 2013, obtaining the top official scores of the challenge (among 53 teams of participants) of event 1 and event 2 concerning fetal heart rate and fetal interbeat intervals estimation section. The developed algorithms are released as open-source on the Physionet website.

  5. Efficient and robust ventricular tachycardia and fibrillation detection method for wearable cardiac health monitoring devices.

    Science.gov (United States)

    Prabhakararao, Eedara; Manikandan, M Sabarimalai

    2016-09-01

    In this Letter, the authors propose an efficient and robust method for automatically determining the VT and VF events in the electrocardiogram (ECG) signal. The proposed method consists of: (i) discrete cosine transform (DCT)-based noise suppression; (ii) addition of bipolar sequence of amplitudes with alternating polarity; (iii) zero-crossing rate (ZCR) estimation-based VTVF detection; and (iv) peak-to-peak interval (PPI) feature based VT/VF discrimination. The proposed method is evaluated using 18,000 episodes of different ECG arrhythmias taken from 6 PhysioNet databases. The method achieves an average sensitivity (Se) of 99.61%, specificity (Sp) of 99.96%, and overall accuracy (OA) of 99.92% in detecting VTVF and non-VTVF episodes by using a ZCR feature. Results show that the method achieves a Se of 100%, Sp of 99.70% and OA of 99.85% for discriminating VT from VF episodes using PPI features extracted from the processed signal. The robustness of the method is tested using different kinds of ECG beats and various types of noises including the baseline wanders, powerline interference and muscle artefacts. Results demonstrate that the proposed method with the ZCR, PPI features can achieve significantly better detection rates as compared with the existing methods.

  6. Adaptive Energy-Efficient Target Detection Based on Mobile Wireless Sensor Networks.

    Science.gov (United States)

    Zou, Tengyue; Li, Zhenjia; Li, Shuyuan; Lin, Shouying

    2017-05-04

    Target detection is a widely used application for area surveillance, elder care, and fire alarms; its purpose is to find a particular object or event in a region of interest. Usually, fixed observing stations or static sensor nodes are arranged uniformly in the field. However, each part of the field has a different probability of being intruded upon; if an object suddenly enters an area with few guardian devices, a loss of detection will occur, and the stations in the safe areas will waste their energy for a long time without any discovery. Thus, mobile wireless sensor networks may benefit from adaptation and pertinence in detection. Sensor nodes equipped with wheels are able to move towards the risk area via an adaptive learning procedure based on Bayesian networks. Furthermore, a clustering algorithm based on k -means++ and an energy control mechanism is used to reduce the energy consumption of nodes. The extended Kalman filter and a voting data fusion method are employed to raise the localization accuracy of the target. The simulation and experimental results indicate that this new system with adaptive energy-efficient methods is able to achieve better performance than the traditional ones.

  7. Tempting To Tag: An Experimental Comparison Of Four Tagging Input Mechanisms

    Directory of Open Access Journals (Sweden)

    Mark Melenhorst

    2010-01-01

    Full Text Available Tagging helps achieve improved indexing and recommendation of resources (e.g., videos or pictures in large data collections. In order to reap the benefits of tagging, people must be persuaded to label the resources they consume. This paper reports on a study in which four different tagging input mechanisms and their effect on users' motivation to tag were compared. The mechanisms consisted of a standard tag input box, a chatbot-like environment, a bookmarking mechanism, and a "tag and vote" game. The results of our experiment show that the use of the nonstandard tagging input mechanisms does not affect users' motivation to tag. In some instances tagging mechanisms were found to distract users from their primary task: consuming resources. Persuading people to tag might be accomplished more effectively by using other motivating tagging mechanisms (e.g., tagging games, or motivation could be created by explaining the usefulness of tagging.

  8. MR Colonography with fecal tagging: Barium vs. barium ferumoxsil

    DEFF Research Database (Denmark)

    Achiam, M.P.; Chabanova, E.; Logager, V.B.

    2008-01-01

    and Methods. Twenty patients referred to CC underwent dark lumen MRC prior to the colonoscopy. Two groups of patients received two different oral contrast agents (barium sulfate and barium sulfate/ferumoxsil) as a laxative-free fecal tagging prior to the MRC. After MRC, the contrast agent was rated...... qualitatively (with the standard method using contrast-to-wall ratio) and subjectively (using a visual analog scale [VAS]) by three different blinded observers. Results. Evaluated both qualitatively and subjectively, the tagging efficiency of barium sulfate/ferumoxsil was significantly better (P ... barium sulfate alone. The VAS method for evaluating the tagging efficiency of contrast agents showed a high correlation (observer 11, r = 0.91) to the standard method using contrast-to-wall ratio and also a high interclass correlation (observer 11 and III = 0.89/0.85). MRC found I of 22 (5%) polyps

  9. Evaluation of Tag Attachments on Small Cetaceans

    Science.gov (United States)

    2013-09-30

    tag on the dorsal fin trailing edge. Dr. Randall Wells of CZS managed the project and coordinated tag production and field tests. All of these...modeling demonstrated that horizontally oriented tags created significantly less drag than vertically oriented tags. Undesirable turbulence was identified...successfully calved in 2013. Follow-up assessments in July found no indication of health problems associated with the tags. The anti-fouling

  10. A new efficient statistical test for detecting variability in the gene expression data.

    Science.gov (United States)

    Mathur, Sunil; Dolo, Samuel

    2008-08-01

    DNA microarray technology allows researchers to monitor the expressions of thousands of genes under different conditions. The detection of differential gene expression under two different conditions is very important in microarray studies. Microarray experiments are multi-step procedures and each step is a potential source of variance. This makes the measurement of variability difficult because approach based on gene-by-gene estimation of variance will have few degrees of freedom. It is highly possible that the assumption of equal variance for all the expression levels may not hold. Also, the assumption of normality of gene expressions may not hold. Thus it is essential to have a statistical procedure which is not based on the normality assumption and also it can detect genes with differential variance efficiently. The detection of differential gene expression variance will allow us to identify experimental variables that affect different biological processes and accuracy of DNA microarray measurements.In this article, a new nonparametric test for scale is developed based on the arctangent of the ratio of two expression levels. Most of the tests available in literature require the assumption of normal distribution, which makes them inapplicable in many situations, and it is also hard to verify the suitability of the normal distribution assumption for the given data set. The proposed test does not require the assumption of the distribution for the underlying population and hence makes it more practical and widely applicable. The asymptotic relative efficiency is calculated under different distributions, which show that the proposed test is very powerful when the assumption of normality breaks down. Monte Carlo simulation studies are performed to compare the power of the proposed test with some of the existing procedures. It is found that the proposed test is more powerful than commonly used tests under almost all the distributions considered in the study. A

  11. DELISHUS: an efficient and exact algorithm for genome-wide detection of deletion polymorphism in autism

    Science.gov (United States)

    Aguiar, Derek; Halldórsson, Bjarni V.; Morrow, Eric M.; Istrail, Sorin

    2012-01-01

    Motivation: The understanding of the genetic determinants of complex disease is undergoing a paradigm shift. Genetic heterogeneity of rare mutations with deleterious effects is more commonly being viewed as a major component of disease. Autism is an excellent example where research is active in identifying matches between the phenotypic and genomic heterogeneities. A considerable portion of autism appears to be correlated with copy number variation, which is not directly probed by single nucleotide polymorphism (SNP) array or sequencing technologies. Identifying the genetic heterogeneity of small deletions remains a major unresolved computational problem partly due to the inability of algorithms to detect them. Results: In this article, we present an algorithmic framework, which we term DELISHUS, that implements three exact algorithms for inferring regions of hemizygosity containing genomic deletions of all sizes and frequencies in SNP genotype data. We implement an efficient backtracking algorithm—that processes a 1 billion entry genome-wide association study SNP matrix in a few minutes—to compute all inherited deletions in a dataset. We further extend our model to give an efficient algorithm for detecting de novo deletions. Finally, given a set of called deletions, we also give a polynomial time algorithm for computing the critical regions of recurrent deletions. DELISHUS achieves significantly lower false-positive rates and higher power than previously published algorithms partly because it considers all individuals in the sample simultaneously. DELISHUS may be applied to SNP array or sequencing data to identify the deletion spectrum for family-based association studies. Availability: DELISHUS is available at http://www.brown.edu/Research/Istrail_Lab/. Contact: Eric_Morrow@brown.edu and Sorin_Istrail@brown.edu Supplementary information: Supplementary data are available at Bioinformatics online. PMID:22689755

  12. Modeling the Fission Fragment Detection Efficiency of the NIFFTE fissionTPC

    Science.gov (United States)

    Bowden, Nathaniel; Niffte Collaboration

    2017-09-01

    The goal of the Neutron Induced Fission Fragment Tracking Experiment (NIFFTE) is to measure neutron-induced fission cross section ratios with unprecedented precision. The NIFFTE Collaboration has designed and built a Time Projection Chamber, the fissionTPC, for this purpose. The detector enables charged particle tracking with full three-dimensional charge cloud reconstruction, allowing for the characterization of fission fragments originating from a thin central target. Quantifying the fission fragment detection efficiency is a central element of these cross section ratio measurements. Here we describe how the wealth of data captured for every fission event allows us to build and validate a detailed Monte Carlo efficiency model. Effects such as anisotropy, fission fragment energy degradation, and target thickness, composition, and roughness must all be taken into account. LLNL-ABS-733618. This work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344.

  13. Frequency interleaving towards spectrally efficient directly detected optical OFDM for next-generation optical access networks.

    Science.gov (United States)

    Mehedy, Lenin; Bakaul, Masuduzzaman; Nirmalathas, Ampalavanapillai

    2010-10-25

    In this paper, we theoretically analyze and demonstrate that spectral efficiency of a conventional direct detection based optical OFDM system (DDO-OFDM) can be improved significantly using frequency interleaving of adjacent DDO-OFDM channels where OFDM signal band of one channel occupies the spectral gap of other channel and vice versa. We show that, at optimum operating condition, the proposed technique can effectively improve the spectral efficiency of the conventional DDO-OFDM system as much as 50%. We also show that such a frequency interleaved DDO-OFDM system, with a bit rate of 48 Gb/s within 25 GHz bandwidth, achieves sufficient power budget after transmission over 25 km single mode fiber to be used in next-generation time-division-multiplexed passive optical networks (TDM-PON). Moreover, by applying 64- quadrature amplitude modulation (QAM), the system can be further scaled up to 96 Gb/s with a power budget sufficient for 1:16 split TDM-PON.

  14. The influence of chemical etching time on efficiency of radon detection using CR-39

    International Nuclear Information System (INIS)

    Reway, Adriana P.; Kappke, Jaqueline; Narloch, Danielle C.; Del Claro, Flavia; Paschuk, Sergei A.; Correa, Janine N.

    2015-01-01

    Natural radiation is the principal source of human exposure to ionizing radiation. Radon is noble radioactive gas that emanates from the soil and rocks entering the atmosphere of dwellings where it could be accumulated. The inhalation of 222 Rn represents a significant health risk. Solid-State Nuclear Track Detectors (SSNTD) represents an efficient method for alpha particle detection and measurements of the activity concentration of 222 Rn. The aim of present work was to study the etching time impact on CR-39 efficiency in radon activity measurements. The investigation was performed using 80 CR-39 detectors, which were exposed to a source of radon. After the exposition, alpha particle tracks development was achieved by chemical etching using 6.25M NaOH solution and ethanol (2%) at 70°C. Etching alpha particle tracks were identified and counted manually using the optical microscope with magnification of 100x and glass overlay mask. The etching time ranged from 7 to 14 hours. The results show that there is an increase in the number of visible tracks with increased etching time. The number of traces obtained for 7 hours and 8 hours of revelation was 1430 +/- 90 and 2090 +/- 160, respectively. However, for etching time of 13 and 14 hours was not observed statistical increase in the number of visible tracks. The number of tracks in this situation was 3630 +/- 180 and 3870 +/- 160 to 13 and 14 hours etching. Thus, for assumed etching parameters, the etching optimal time was observed 14 hours. (author)

  15. Efficient Data Collection by Mobile Sink to Detect Phenomena in Internet of Things

    Directory of Open Access Journals (Sweden)

    Amany Abu Safia

    2017-10-01

    Full Text Available With the rapid development of Internet of Things (IoT, more and more static and mobile sensors are being deployed for sensing and tracking environmental phenomena, such as fire, oil spills and air pollution. As these sensors are usually battery-powered, energy-efficient algorithms are required to extend the sensors’ lifetime. Moreover, forwarding sensed data towards a static sink causes quick battery depletion of the sinks’ nearby sensors. Therefore, in this paper, we propose a distributed energy-efficient algorithm, called the Hilbert-order Collection Strategy (HCS, which uses a mobile sink (e.g., drone to collect data from a mobile wireless sensor network (mWSN and detect environmental phenomena. The mWSN consists of mobile sensors that sense environmental data. These mobile sensors self-organize themselves into groups. The sensors of each group elect a group head (GH, which collects data from the mobile sensors in its group. Periodically, a mobile sink passes by the locations of the GHs (data collection path to collect their data. The collected data are aggregated to discover a global phenomenon. To shorten the data collection path, which results in reducing the energy cost, the mobile sink establishes the path based on the order of Hilbert values of the GHs’ locations. Furthermore, the paper proposes two optimization techniques for data collection to further reduce the energy cost of mWSN and reduce the data loss.

  16. Neutral B meson flavor tagging

    CERN Document Server

    Wilson, R J

    2001-01-01

    We present an investigation of the use of net charge and kaon identification to tag the flavor of neutral B mesons. The net charge of the neutral B meson decay products is zero if all charged particles are used and slightly non-zero if only undiscriminated hadronic final states are used. The net charge of the kaons alone correctly tags the identity of the neutral meson in at least a third of all decays. We have parametrized the particle identification capability of several techniques, such as dE/dx in time projection chambers, LEP/SLC ring-imaging chambers and an enhanced BaBar DIRC. Using these parametrisations we compare the relative tagging power of each technique to that of an ideal detector. (8 refs).

  17. A Framework and Classification for Fault Detection Approaches in Wireless Sensor Networks with an Energy Efficiency Perspective

    DEFF Research Database (Denmark)

    Zhang, Yue; Dragoni, Nicola; Wang, Jiangtao

    2015-01-01

    Wireless Sensor Networks (WSNs) are more and more considered a key enabling technology for the realisation of the Internet of Things (IoT) vision. With the long term goal of designing fault-tolerant IoT systems, this paper proposes a fault detection framework for WSNs with the perspective of energy...... approaches for the comparison of several characteristics, namely, energy efficiency, correlation model, evaluation method, and detection accuracy. The design guidelines given in this paper aim at providing an insight into better design of energy-efficient detection approaches in resource-constraint WSNs....

  18. Enhanced detection and study of murine norovirus-1 using a more efficient microglial cell line

    Directory of Open Access Journals (Sweden)

    Lu Yuanan

    2009-11-01

    Full Text Available Abstract Background Human Noroviruses are the predominant cause of non-bacterial gastroenteritis worldwide. To facilitate prevention and control, a norovirus isolated from mice can provide a model to understand human noroviruses. To establish optimal viral infectivity conditions for murine noroviruses, several cell lines of hematopoietic lineage, including murine BV-2, RAW 264.7, and TIB, as well as human CHME-5, were tested comparatively for their sensitivity to murine norovirus-1. Results Except for CHME-5, all three murine-derived cell lines were susceptible to MNV infection. Viral infection of these cells was confirmed by RT-PCR. Using both viral plaque and replication assays, BV-2 and RAW 264.7 cells were determined to have comparable sensitivities to MNV-1 infection. Comparisons of cell growth characteristics, general laboratory handling and potential in-field applications suggest the use of BV-2 to be more advantageous. Conclusion Results obtained from these studies demonstrate that an immortalized microglial cell line can support MNV-1 replication and provides a more efficient method to detect and study murine noroviruses, facilitating future investigations using MNV-1 as a model to study, detect, and control Human Norovirus.

  19. Efficient deep learning model for mitosis detection using breast histopathology images.

    Science.gov (United States)

    Saha, Monjoy; Chakraborty, Chandan; Racoceanu, Daniel

    2018-03-01

    Mitosis detection is one of the critical factors of cancer prognosis, carrying significant diagnostic information required for breast cancer grading. It provides vital clues to estimate the aggressiveness and the proliferation rate of the tumour. The manual mitosis quantification from whole slide images is a very labor-intensive and challenging task. The aim of this study is to propose a supervised model to detect mitosis signature from breast histopathology WSI images. The model has been designed using deep learning architecture with handcrafted features. We used handcrafted features issued from previous medical challenges MITOS @ ICPR 2012, AMIDA-13 and projects (MICO ANR TecSan) expertise. The deep learning architecture mainly consists of five convolution layers, four max-pooling layers, four rectified linear units (ReLU), and two fully connected layers. ReLU has been used after each convolution layer as an activation function. Dropout layer has been included after first fully connected layer to avoid overfitting. Handcrafted features mainly consist of morphological, textural and intensity features. The proposed architecture has shown to have an improved 92% precision, 88% recall and 90% F-score. Prospectively, the proposed model will be very beneficial in routine exam, providing pathologists with efficient and - as we will prove - effective second opinion for breast cancer grading from whole slide images. Last but not the least, this model could lead junior and senior pathologists, as medical researchers, to a superior understanding and evaluation of breast cancer stage and genesis. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Experimental Study on Inkjet-Printed Passive UHF RFID Tags on Versatile Paper-Based Substrates

    Directory of Open Access Journals (Sweden)

    Han He

    2016-01-01

    Full Text Available We present the possibilities and challenges of passive UHF RFID tag antennas manufactured by inkjet printing silver nanoparticle ink on versatile paper-based substrates. The most efficient manufacturing parameters, such as the pattern resolution, were determined and the optimal number of printed layers was evaluated for each substrate material. Next, inkjet-printed passive UHF RFID tags were fabricated on each substrate with the optimized parameters and number of layers. According to our measurements, the tags on different paper substrates showed peak read ranges of 4–6.5 meters and the tags on different cardboard substrates exhibited peak read ranges of 2–6 meters. Based on their wireless performance, these inkjet-printed paper-based passive UHF RFID tags are sufficient for many future wireless applications and comparable to tags fabricated on more traditional substrates, such as polyimide.

  1. Pothole tagging system

    CSIR Research Space (South Africa)

    Joubert, D

    2011-11-01

    Full Text Available of complicated algorithms to produce 3-D data. A survey is presented in [8] on various automated distress detection systems that utilise machine vision. In [9] a digital camera is used, while in [10] a structured light approach, utilising both a laser and a... drawing of the sensor rack. will be used. Such a high performance laptop is needed due to the steep processing and memory requirements imposed by the high rate of data generation from both the high speed camera and the Kinect. The laptop is also...

  2. Interference Helps to Equalize the Read Range and Reduce False Positives of Passive RFID Tags

    DEFF Research Database (Denmark)

    Krigslund, Rasmus; Popovski, Petar; Pedersen, Gert Frølund

    2012-01-01

    In various applications of RFID systems a reader should reliably get the ID of the tags that are within a bounded proximity region, termed the interrogation zone. This gives rise to two types of errors 1) False Negative Detections (FNDs), when tags within the intended interrogation zone cannot...... interference enables design of well-defined interrogation zones for passive RFID systems....

  3. Effective detective quantum efficiency for two mammography systems: Measurement and comparison against established metrics

    Energy Technology Data Exchange (ETDEWEB)

    Salvagnini, Elena [UZ Gasthuisberg, Medical Imaging Research Center and Department of Radiology, Herestraat 49, B-3000 Leuven, Belgium and SCK-CEN, Boeretang 200, B-2400 Mol (Belgium); Bosmans, Hilde; Marshall, Nicholas W. [UZ Gasthuisberg, Medical Imaging Research Center and Department of Radiology, Herestraat 49, B-3000 Leuven (Belgium); Struelens, Lara [SCK-CEN, Boeretang 200, B-2400 Mol (Belgium)

    2013-10-15

    Purpose: The aim of this paper was to illustrate the value of the new metric effective detective quantum efficiency (eDQE) in relation to more established measures in the optimization process of two digital mammography systems. The following metrics were included for comparison against eDQE: detective quantum efficiency (DQE) of the detector, signal difference to noise ratio (SdNR), and detectability index (d′) calculated using a standard nonprewhitened observer with eye filter.Methods: The two systems investigated were the Siemens MAMMOMAT Inspiration and the Hologic Selenia Dimensions. The presampling modulation transfer function (MTF) required for the eDQE was measured using two geometries: a geometry containing scattered radiation and a low scatter geometry. The eDQE, SdNR, and d′ were measured for poly(methyl methacrylate) (PMMA) thicknesses of 20, 40, 60, and 70 mm, with and without the antiscatter grid and for a selection of clinically relevant target/filter (T/F) combinations. Figures of merit (FOMs) were then formed from SdNR and d′ using the mean glandular dose as the factor to express detriment. Detector DQE was measured at energies covering the range of typical clinically used spectra.Results: The MTF measured in the presence of scattered radiation showed a large drop at low spatial frequency compared to the low scatter method and led to a corresponding reduction in eDQE. The eDQE for the Siemens system at 1 mm{sup −1} ranged between 0.15 and 0.27, depending on T/F and grid setting. For the Hologic system, eDQE at 1 mm{sup −1} varied from 0.15 to 0.32, again depending on T/F and grid setting. The eDQE results for both systems showed that the grid increased the system efficiency for PMMA thicknesses of 40 mm and above but showed only small sensitivity to T/F setting. While results of the SdNR and d′ based FOMs confirmed the eDQE grid position results, they were also more specific in terms of T/F selection. For the Siemens system at 20 mm PMMA

  4. Effective detective quantum efficiency for two mammography systems: measurement and comparison against established metrics.

    Science.gov (United States)

    Salvagnini, Elena; Bosmans, Hilde; Struelens, Lara; Marshall, Nicholas W

    2013-10-01

    The aim of this paper was to illustrate the value of the new metric effective detective quantum efficiency (eDQE) in relation to more established measures in the optimization process of two digital mammography systems. The following metrics were included for comparison against eDQE: detective quantum efficiency (DQE) of the detector, signal difference to noise ratio (SdNR), and detectability index (d') calculated using a standard nonprewhitened observer with eye filter. The two systems investigated were the Siemens MAMMOMAT Inspiration and the Hologic Selenia Dimensions. The presampling modulation transfer function (MTF) required for the eDQE was measured using two geometries: a geometry containing scattered radiation and a low scatter geometry. The eDQE, SdNR, and d' were measured for poly(methyl methacrylate) (PMMA) thicknesses of 20, 40, 60, and 70 mm, with and without the antiscatter grid and for a selection of clinically relevant target/filter (T/F) combinations. Figures of merit (FOMs) were then formed from SdNR and d' using the mean glandular dose as the factor to express detriment. Detector DQE was measured at energies covering the range of typical clinically used spectra. The MTF measured in the presence of scattered radiation showed a large drop at low spatial frequency compared to the low scatter method and led to a corresponding reduction in eDQE. The eDQE for the Siemens system at 1 mm(-1) ranged between 0.15 and 0.27, depending on T/F and grid setting. For the Hologic system, eDQE at 1 mm(-1) varied from 0.15 to 0.32, again depending on T/F and grid setting. The eDQE results for both systems showed that the grid increased the system efficiency for PMMA thicknesses of 40 mm and above but showed only small sensitivity to T/F setting. While results of the SdNR and d' based FOMs confirmed the eDQE grid position results, they were also more specific in terms of T/F selection. For the Siemens system at 20 mm PMMA, the FOMs indicated Mo/Mo (grid out) as

  5. Novel expressed sequence tag- simple sequence repeats (EST ...

    African Journals Online (AJOL)

    Using different bioinformatic criteria, the SUCEST database was used to mine for simple sequence repeat (SSR) markers. Among 42,189 clusters, 1,425 expressed sequence tag- simple sequence repeats (EST-SSRs) were identified in silico. Trinucleotide repeats were the most abundant SSRs detected. Of 212 primer pairs ...

  6. The influence of chemical etching time on efficiency of radon detection using CR-39

    Energy Technology Data Exchange (ETDEWEB)

    Reway, Adriana P.; Kappke, Jaqueline; Narloch, Danielle C., E-mail: adrireway@hotmail.com, E-mail: jaquelinekappke@gmail.com, E-mail: daninarloch@hotmail.com [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil). Departamento Academico de Fisica; Del Claro, Flavia; Paschuk, Sergei A., E-mail: flaviadelclaro@gmail.com, E-mail: spaschuk@gmail.com [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil). Programa de Pos-Graduaca em Engenharia Eletrica e Informatica Industrial; Correa, Janine N., E-mail: janine_nicolosi@hotmail.com [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil). Departamento Academico de Construcao Civil

    2015-07-01

    Natural radiation is the principal source of human exposure to ionizing radiation. Radon is noble radioactive gas that emanates from the soil and rocks entering the atmosphere of dwellings where it could be accumulated. The inhalation of {sup 222}Rn represents a significant health risk. Solid-State Nuclear Track Detectors (SSNTD) represents an efficient method for alpha particle detection and measurements of the activity concentration of {sup 222}Rn. The aim of present work was to study the etching time impact on CR-39 efficiency in radon activity measurements. The investigation was performed using 80 CR-39 detectors, which were exposed to a source of radon. After the exposition, alpha particle tracks development was achieved by chemical etching using 6.25M NaOH solution and ethanol (2%) at 70°C. Etching alpha particle tracks were identified and counted manually using the optical microscope with magnification of 100x and glass overlay mask. The etching time ranged from 7 to 14 hours. The results show that there is an increase in the number of visible tracks with increased etching time. The number of traces obtained for 7 hours and 8 hours of revelation was 1430 +/- 90 and 2090 +/- 160, respectively. However, for etching time of 13 and 14 hours was not observed statistical increase in the number of visible tracks. The number of tracks in this situation was 3630 +/- 180 and 3870 +/- 160 to 13 and 14 hours etching. Thus, for assumed etching parameters, the etching optimal time was observed 14 hours. (author)

  7. A comparison of digital radiography systems in terms of effective detective quantum efficiency

    Energy Technology Data Exchange (ETDEWEB)

    Bertolini, Marco; Nitrosi, Andrea; Rivetti, Stefano; Lanconelli, Nico; Pattacini, Pierpaolo; Ginocchi, Vladimiro; Iori, Mauro [Department of Advanced Technology, Medical Physics Unit, Azienda Ospedaliera ASMN, Istituto di Ricovero e Cura a Carattere Scientifico, Reggio Emilia 42123 (Italy); Fisica Medica, Ospedale di Sassuolo S.p.A., Modena 41049 (Italy); Alma Mater Studiorum, Physics Department, University of Bologna, Bologna 40127 (Italy); Department of Diagnostic Imaging, Radiology Unit, Azienda Ospedaliera ASMN, Istituto di Ricovero e Cura a Carattere Scientifico, Reggio Emilia 42123 (Italy); Department of Diagnostic Imaging, Radiology Unit, Azienda USL, Reggio Emilia 42122 (Italy); Department of Advanced Technology, Medical Physics Unit, Azienda Ospedaliera ASMN, Istituto di Ricovero e Cura a Carattere Scientifico, Reggio Emilia 42123 (Italy)

    2012-05-15

    Purpose: The purpose of this study is to compare digital radiography systems using the metric effective detective quantum efficiency (eDQE), which better reflects digital radiography imaging system performance under clinical operating conditions, in comparison with conventional metrics such as modulation transfer function (MTF), normalized noise power spectra (NNPS), and detective quantum efficiency (DQE). Methods: The eDQE was computed by the calculation of the MTF, the NNPS, the phantom attenuation and scatter, and estimation of x-ray flux. The physical characterization of the systems was obtained with the standard beam conditions RQA5 and RQA9, using the PA Chest phantom proposed by AAPM Report no. 31 simulating the attenuation and scatter characteristics of the adult human thorax. The MTF (eMTF) was measured by using an edge test placed at the frontal surface of the phantom, the NNPS (eNNPS) was calculated from images of the phantom acquired at three different exposure levels covering the operating range of the system (E{sub 0}, which is the exposure at which a system is normally operated, 1/3 E{sub 0}, and 3 E0), and scatter measurements were assessed by using a beam-stop technique. The integral of DQE (IDQE) and eDQE (IeDQE) was calculated over the whole spatial frequency range. Results: The eMTF results demonstrate degradation due to magnification and the presence of scattered radiation. The eNNPS was influenced by the grid presence, and in some systems, it contained structured noise. At typical clinical exposure levels, the magnitude of eDQE(0) with respect to DQE(0) at RQA9 beam conditions was 13%, 17%, 16%, 36%, and 24%, respectively, for Carestream DRX-1, Carestream DRX-1C, Carestream Direct View CR975, Philips Digital Diagnost VM, and GE Revolution XR/d. These results were confirmed by the ratio of IeDQE and IDQE in the same conditions. Conclusions: The authors confirm the robustness and reproducibility of the eDQE method. As expected, the DR systems

  8. A comparison of digital radiography systems in terms of effective detective quantum efficiency

    International Nuclear Information System (INIS)

    Bertolini, Marco; Nitrosi, Andrea; Rivetti, Stefano; Lanconelli, Nico; Pattacini, Pierpaolo; Ginocchi, Vladimiro; Iori, Mauro

    2012-01-01

    Purpose: The purpose of this study is to compare digital radiography systems using the metric effective detective quantum efficiency (eDQE), which better reflects digital radiography imaging system performance under clinical operating conditions, in comparison with conventional metrics such as modulation transfer function (MTF), normalized noise power spectra (NNPS), and detective quantum efficiency (DQE). Methods: The eDQE was computed by the calculation of the MTF, the NNPS, the phantom attenuation and scatter, and estimation of x-ray flux. The physical characterization of the systems was obtained with the standard beam conditions RQA5 and RQA9, using the PA Chest phantom proposed by AAPM Report no. 31 simulating the attenuation and scatter characteristics of the adult human thorax. The MTF (eMTF) was measured by using an edge test placed at the frontal surface of the phantom, the NNPS (eNNPS) was calculated from images of the phantom acquired at three different exposure levels covering the operating range of the system (E 0 , which is the exposure at which a system is normally operated, 1/3 E 0 , and 3 E0), and scatter measurements were assessed by using a beam-stop technique. The integral of DQE (IDQE) and eDQE (IeDQE) was calculated over the whole spatial frequency range. Results: The eMTF results demonstrate degradation due to magnification and the presence of scattered radiation. The eNNPS was influenced by the grid presence, and in some systems, it contained structured noise. At typical clinical exposure levels, the magnitude of eDQE(0) with respect to DQE(0) at RQA9 beam conditions was 13%, 17%, 16%, 36%, and 24%, respectively, for Carestream DRX-1, Carestream DRX-1C, Carestream Direct View CR975, Philips Digital Diagnost VM, and GE Revolution XR/d. These results were confirmed by the ratio of IeDQE and IDQE in the same conditions. Conclusions: The authors confirm the robustness and reproducibility of the eDQE method. As expected, the DR systems performed

  9. Efficient sequential Bayesian inference method for real-time detection and sorting of overlapped neural spikes.

    Science.gov (United States)

    Haga, Tatsuya; Fukayama, Osamu; Takayama, Yuzo; Hoshino, Takayuki; Mabuchi, Kunihiko

    2013-09-30

    Overlapping of extracellularly recorded neural spike waveforms causes the original spike waveforms to become hidden and merged, confounding the real-time detection and sorting of these spikes. Methods proposed for solving this problem include using a multi-trode or placing a restriction on the complexity of overlaps. In this paper, we propose a rapid sequential method for the robust detection and sorting of arbitrarily overlapped spikes recorded with arbitrary types of electrodes. In our method, the probabilities of possible spike trains, including those that are overlapping, are evaluated by sequential Bayesian inference based on probabilistic models of spike-train generation and extracellular voltage recording. To reduce the high computational cost inherent in an exhaustive evaluation, candidates with low probabilities are considered as impossible candidates and are abolished at each sampling time to limit the number of candidates in the next evaluation. In addition, the data from a few subsequent sampling times are considered and used to calculate the "look-ahead probability", resulting in improved calculation efficiency due to a more rapid elimination of candidates. These sufficiently reduce computational time to enable real-time calculation without impairing performance. We assessed the performance of our method using simulated neural signals and actual neural signals recorded in primary cortical neurons cultured on a multi-electrode array. Our results demonstrated that our computational method could be applied in real-time with a delay of less than 10 ms. The estimation accuracy was higher than that of a conventional spike sorting method, particularly for signals with multiple overlapping spikes. Copyright © 2013 Elsevier B.V. All rights reserved.

  10. Quantifying Visual-Representativeness of Social Image Tags Using Image Tag Clarity

    Science.gov (United States)

    Sun, Aixin; Bhowmick, Sourav S.

    Tags associated with images in various social media sharing web sites are valuable information source for superior image retrieval experiences. Due to the nature of tagging, many tags associated with images are not visually descriptive. In this chapter, we propose Image Tag Clarity to evaluate the effectiveness of a tag in describing the visual content of its annotated images, which is also known as the image tag visual-representativeness. It is measured by computing the zero-mean normalized distance between the tag language model estimated from the images annotated by the tag and the collection language model. The tag/collection language models are derived from the bag of visual-word local content features of the images. The visual-representative tags that are commonly used to annotate visually similar images are given high tag clarity scores. Evaluated on a large real-world dataset containing more than 269K images and their associated tags, we show that the image tag clarity score can effectively identify the visual-representative tags from all tags contributed by users. Based on the tag clarity scores, we have made a few interesting observations that could be used to support many tag-based applications.

  11. Effect of finite detection efficiency on the observation of the dipole-dipole interaction of a few Rydberg atoms

    International Nuclear Information System (INIS)

    Ryabtsev, I. I.; Tretyakov, D. B.; Beterov, I. I.; Entin, V. M.

    2007-01-01

    We have developed a simple analytical model describing multiatom signals that are measured in experiments on dipole-dipole interaction at resonant collisions of a few Rydberg atoms. It has been shown that finite efficiency of the selective field-ionization detector leads to the mixing up of the spectra of resonant collisions registered for various numbers of Rydberg atoms. The formulas which help to estimate an appropriate mean Rydberg atom number for a given detection efficiency are presented. We have found that a measurement of the relation between the amplitudes of collisional resonances observed in the one- and two-atom signals provides a straightforward determination of the absolute detection efficiency and mean Rydberg atom number. We also performed a testing experiment on resonant collisions in a small excitation volume of a sodium atomic beam. The resonances observed for 1-4 detected Rydberg atoms have been analyzed and compared with theory

  12. Application of cine MRI-tagging method to aortic dessection

    International Nuclear Information System (INIS)

    Yoshioka, Kunihiro; Takahashi, Tsuneo; Kamata, Hiroyuki; Kikuchi, Kenichi; Yamaguchi, Kojiro.

    1992-01-01

    For the evaluation of aortic dissection, ECG-gated SE or cine MR imaging has been usually performed. However, detection of slow flow in the false lumen and differentiation between mural thrombus and slow flow are sometimes difficult. Because paradoxical enhancement due to slow blood flow simulates thrombus. We performed cine MR imaging with persaturation tagging, which clearly showed differentiation between thrombus and paradoxical enhancement. We concluded that cine MR imaging with tagging method was useful to evaluate the slow flow and thrombus in the false lumen. (author)

  13. Theory and experiments on Peano and Hilbert curve RFID tags

    Science.gov (United States)

    McVay, John; Hoorfar, Ahmad; Engheta, Nader

    2006-05-01

    Recently, there has been considerable interest in the area of Radio Frequency Identification (RFID) and Radio Frequency Tagging (RFTAG). This emerging area of interest can be applied for inventory control (commercial) as well as friend/foe identification (military) to name but a few. The current technology can be broken down into two main groups, namely passive and active RFID tags. Utilization of Space-Filling Curve (SFC) geometries, such as the Peano and Hilbert curves, has been recently investigated for use in completely passive RFID applications [1, 2]. In this work, we give an overview of our work on the space-filling curves and the potential for utilizing the electrically small, resonant characteristics of these curves for use in RFID technologies with an emphasis on the challenging issues involved when attempting to tag conductive objects. In particular, we investigate the possible use of these tags in conjunction with high impedance ground-planes made of Hilbert or Peano curve inclusions [3, 4] to develop electrically small RFID tags that may also radiate efficiently, within close proximity of large conductive objects [5].

  14. HMSRP Hawaiian Monk Seal Tag Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains records for all tags applied to Hawaiian monk seals since 1981. These tags were applied by PSD personnel and cooperating scientists as part of...

  15. Satellite Tags- Guam/CNMI EEZ

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Satellite tagging was implemented in 2013. Satellite tagging is conducted using a Dan Inject air rifle and deployment arrows designed by Wildlife Computers. Two...

  16. A Novel Security Method For RFID Tags

    Directory of Open Access Journals (Sweden)

    Imran Ali Jokhio

    2012-07-01

    Full Text Available RFID (Radio Frequency Identification tags use light weighted security methods because of cost constraints. In this paper a lightweight security method is investigated and proved that it significantly lacks in protecting RFID tags against simple cloning attack. In order to protect RFID tag from cloning attacks a novel security method is proposed in this paper. The proposed security method provides high level computational difficulty against the three basic attacking techniques, i.e. eavesdropping, replay and man in the middle. In order to clone a tag, attacker eavesdrops the tag responses and creates a replica of the tag. The novel security method presented in this paper increases the hardness to avoid guessing tag secrets resulting in a conditional none clone able tags. The proposed security method is also evaluated using propositional logic proofs to demonstrate the level of security it can provide.

  17. The Complex Dynamics of Collaborative Tagging

    NARCIS (Netherlands)

    H. Halpin; V. Robu (Valentin); H. Shepherd

    2007-01-01

    textabstractThe debate within the Web community over the optimal means by which to organize information often pits formalized classifications against distributed collaborative tagging systems. A number of questions remain unanswered, however, regarding the nature of collaborative tagging systems

  18. Efficient feature selection using a hybrid algorithm for the task of epileptic seizure detection

    Science.gov (United States)

    Lai, Kee Huong; Zainuddin, Zarita; Ong, Pauline

    2014-07-01

    Feature selection is a very important aspect in the field of machine learning. It entails the search of an optimal subset from a very large data set with high dimensional feature space. Apart from eliminating redundant features and reducing computational cost, a good selection of feature also leads to higher prediction and classification accuracy. In this paper, an efficient feature selection technique is introduced in the task of epileptic seizure detection. The raw data are electroencephalography (EEG) signals. Using discrete wavelet transform, the biomedical signals were decomposed into several sets of wavelet coefficients. To reduce the dimension of these wavelet coefficients, a feature selection method that combines the strength of both filter and wrapper methods is proposed. Principal component analysis (PCA) is used as part of the filter method. As for wrapper method, the evolutionary harmony search (HS) algorithm is employed. This metaheuristic method aims at finding the best discriminating set of features from the original data. The obtained features were then used as input for an automated classifier, namely wavelet neural networks (WNNs). The WNNs model was trained to perform a binary classification task, that is, to determine whether a given EEG signal was normal or epileptic. For comparison purposes, different sets of features were also used as input. Simulation results showed that the WNNs that used the features chosen by the hybrid algorithm achieved the highest overall classification accuracy.

  19. Practical expressions describing detective quantum efficiency in flat-panel detectors

    Science.gov (United States)

    Kim, H. K.

    2011-11-01

    In radiology, image quality excellence is a balance between system performance and patient dose, hence x-ray systems must be designed to ensure the maximum image quality is obtained for the lowest consistent dose. The concept of detective quantum efficiency (DQE) is widely used to quantify, understand, measure, and predict the performance of x-ray detectors and imaging systems. Cascaded linear-systems theory can be used to estimate DQE based on the system design parameters and this theoretical DQE can be utilized for determining the impact of various physical processes, such as secondary quantum sinks, noise aliasing, reabsorption noise, and others. However, the prediction of DQE usually requires tremendous efforts to determine each parameter consisting of the cascaded linear-systems model. In this paper, practical DQE formalisms assessing both the photoconductor- and scintillator-based flat-panel detectors under quantum-noise-limited operation are described. The developed formalisms are experimentally validated and discussed for their limits. The formalisms described in this paper would be helpful for the rapid prediction of the DQE performances of developing systems as well as the optimal design of systems.

  20. Set-up and methods for SiPM Photo-Detection Efficiency measurements

    International Nuclear Information System (INIS)

    Zappalà, G.; Acerbi, F.; Ferri, A.; Gola, A.; Paternoster, G.; Zorzi, N.; Piemonte, C.

    2016-01-01

    In this work, a compact set-up and three different methods to measure the Photo-Detection Efficiency (PDE) of Silicon Photomultipliers (SiPMs) and Single-Photon Avalanche Diodes (SPADs) are presented. The methods, based on either continuous or pulsed light illumination, are discussed in detail and compared in terms of measurement precision and time. For the SiPM, these methods have the feature of minimizing the effect of both the primary and correlated noise on the PDE estimation. The PDE of SiPMs (produced at FBK, Trento, Italy) was measured in a range from UV to NIR, obtaining similar results with all the methods. Furthermore, the advantages of measuring, when possible, the PDE of SPADs (of the same technology and with the same layout of a single SiPM cell) instead of larger devices are also discussed and a direct comparison between measurement results is shown. Using a SPAD, it is possible to reduce the measurement complexity and uncertainty since the correlated noise sources are reduced with respect to the SiPM case.

  1. Performance of SEM scintillation detector evaluated by modulation transfer function and detective quantum efficiency function.

    Science.gov (United States)

    Bok, Jan; Schauer, Petr

    2014-01-01

    In the paper, the SEM detector is evaluated by the modulation transfer function (MTF) which expresses the detector's influence on the SEM image contrast. This is a novel approach, since the MTF was used previously to describe only the area imaging detectors, or whole imaging systems. The measurement technique and calculation of the MTF for the SEM detector are presented. In addition, the measurement and calculation of the detective quantum efficiency (DQE) as a function of the spatial frequency for the SEM detector are described. In this technique, the time modulated e-beam is used in order to create well-defined input signal for the detector. The MTF and DQE measurements are demonstrated on the Everhart-Thornley scintillation detector. This detector was alternated using the YAG:Ce, YAP:Ce, and CRY18 single-crystal scintillators. The presented MTF and DQE characteristics show good imaging properties of the detectors with the YAP:Ce or CRY18 scintillator, especially for a specific type of the e-beam scan. The results demonstrate the great benefit of the description of SEM detectors using the MTF and DQE. In addition, point-by-point and continual-sweep e-beam scans in SEM were discussed and their influence on the image quality was revealed using the MTF. © 2013 Wiley Periodicals, Inc.

  2. The bremsstrahlung tagged photon beam in Hall B at JLab

    CERN Document Server

    Sober, D I; Longhi, A; Matthews, S K; O'Brien, J T; Berman, B L; Briscoe, W J; Cole, P L; Connelly, J P; Dodge, W R; Murphy, L Y; Philips, S A; Dugger, M K; Lawrence, D; Ritchie, B G; Smith, E S; Lambert, J M; Anciant, E; Audit, G; Auger, T; Marchand, C; Klusman, M; Napolitano, J; Khandaker, M A; Salgado, C W; Sarty, A J

    2000-01-01

    We describe the design and commissioning of the photon tagging beamline installed in experimental Hall B at the Thomas Jefferson National Accelerator Facility (JLab). This system can tag photon energies over a range from 20% to 95% of the incident electron energy, and is capable of operation with beam energies up to 6.1 GeV. A single dipole magnet is combined with a hodoscope containing two planar arrays of plastic scintillators to detect energy-degraded electrons from a thin bremsstrahlung radiator. The first layer of 384 partially overlapping small scintillators provides photon energy resolution, while the second layer of 61 larger scintillators provides the timing resolution necessary to form a coincidence with the corresponding nuclear interaction triggered by the tagged photon. The definitions of overlap channels in the first counter plane and of geometric correlation between the two planes are determined using digitized time information from the individual counters. Auxiliary beamline devices are briefl...

  3. A suite of standard post-tagging evaluation metrics can help assess tag retention for field-based fish telemetry research

    Science.gov (United States)

    Gerber, Kayla M.; Mather, Martha E.; Smith, Joseph M.

    2017-01-01

    Telemetry can inform many scientific and research questions if a context exists for integrating individual studies into the larger body of literature. Creating cumulative distributions of post-tagging evaluation metrics would allow individual researchers to relate their telemetry data to other studies. Widespread reporting of standard metrics is a precursor to the calculation of benchmarks for these distributions (e.g., mean, SD, 95% CI). Here we illustrate five types of standard post-tagging evaluation metrics using acoustically tagged Blue Catfish (Ictalurus furcatus) released into a Kansas reservoir. These metrics included: (1) percent of tagged fish detected overall, (2) percent of tagged fish detected daily using abacus plot data, (3) average number of (and percent of available) receiver sites visited, (4) date of last movement between receiver sites (and percent of tagged fish moving during that time period), and (5) number (and percent) of fish that egressed through exit gates. These metrics were calculated for one to three time periods: early ( 5 days early in the study. On average, tagged Blue Catfish visited 9 (50%) and 13 (72%) of 18 within-reservoir receivers early and at the end of the study, respectively. At the end of the study, 73% of all tagged fish were detected moving between receivers. Creating statistical benchmarks for individual metrics can provide useful reference points. In addition, combining multiple metrics can inform ecology and research design. Consequently, individual researchers and the field of telemetry research can benefit from widespread, detailed, and standard reporting of post-tagging detection metrics.

  4. Plastic-casting intrinsic-surface unique identifier (tag)

    International Nuclear Information System (INIS)

    Palm, R.G.; De Volpi, A.

    1995-04-01

    This report describes the development of an authenticated intrinsic-surf ace tagging method for unique- identification of controlled items. Although developed for control of items limited by an arms control treaty, this method has other potential applications to keep track of critical or high-value items. Each tag (unique-identifier) consists of the intrinsic, microscopic surface topography of a small designated area on a controlled item. It is implemented by making a baseline plastic casting of the designated tag area and usually placing a cover (for example, a bar-code label) over this area to protect the surface from environmental alteration. The plastic casting is returned to a laboratory and prepared for high-resolution scanning electron microscope imaging. Several images are digitized and stored for use as a standard for authentication of castings taken during future inspections. Authentication is determined by numerically comparing digital images. Commercially available hardware and software are used for this tag. Tag parameters are optimized, so unique casting images are obtained from original surfaces, and images obtained from attempted duplicate surfaces are detected. This optimization uses the modulation transfer function, a first principle of image analysis, to determine the parameters. Surface duplication experiments confirmed the optimization

  5. Plastic-casting intrinsic-surface unique identifier (tag)

    Energy Technology Data Exchange (ETDEWEB)

    Palm, R.G.; De Volpi, A.

    1995-04-01

    This report describes the development of an authenticated intrinsic-surf ace tagging method for unique- identification of controlled items. Although developed for control of items limited by an arms control treaty, this method has other potential applications to keep track of critical or high-value items. Each tag (unique-identifier) consists of the intrinsic, microscopic surface topography of a small designated area on a controlled item. It is implemented by making a baseline plastic casting of the designated tag area and usually placing a cover (for example, a bar-code label) over this area to protect the surface from environmental alteration. The plastic casting is returned to a laboratory and prepared for high-resolution scanning electron microscope imaging. Several images are digitized and stored for use as a standard for authentication of castings taken during future inspections. Authentication is determined by numerically comparing digital images. Commercially available hardware and software are used for this tag. Tag parameters are optimized, so unique casting images are obtained from original surfaces, and images obtained from attempted duplicate surfaces are detected. This optimization uses the modulation transfer function, a first principle of image analysis, to determine the parameters. Surface duplication experiments confirmed the optimization.

  6. Endogenous gene tagging with fluorescent proteins.

    Science.gov (United States)

    Fetter, John; Samsonov, Andrey; Zenser, Nathan; Zhang, Fan; Zhang, Hongyi; Malkov, Dmitry

    2015-01-01

    Human genome manipulation has become a powerful tool for understanding the mechanisms of numerous diseases including cancer. Inserting reporter sequences in the desired locations in the genome of a cell can allow monitoring of endogenous activities of disease related genes. Native gene expression and regulation is preserved in these knock-in cells in contrast to cell lines with target overexpression under an exogenous promoter as in the case of transient transfection or stable cell lines with random integration. The fusion proteins created using the modern genome editing tools are expressed at their physiological level and thus are more likely to retain the characteristic expression profile of the endogenous proteins in the cell. Unlike biochemical assays or immunostaining, using a tagged protein under endogenous regulation avoids fixation artifacts and allows detection of the target's activity in live cells. Multiple gene targets could be tagged in a single cell line allowing for the creation of effective cell-based assays for compound screening to discover novel drugs.

  7. Infrared tag and track technique

    Science.gov (United States)

    Partin, Judy K.; Stone, Mark L.; Slater, John; Davidson, James R.

    2007-12-04

    A method of covertly tagging an object for later tracking includes providing a material capable of at least one of being applied to the object and being included in the object, which material includes deuterium; and performing at least one of applying the material to the object and including the material in the object in a manner in which in the appearance of the object is not changed, to the naked eye.

  8. Approximation properties of haplotype tagging

    Directory of Open Access Journals (Sweden)

    Dreiseitl Stephan

    2006-01-01

    Full Text Available Abstract Background Single nucleotide polymorphisms (SNPs are locations at which the genomic sequences of population members differ. Since these differences are known to follow patterns, disease association studies are facilitated by identifying SNPs that allow the unique identification of such patterns. This process, known as haplotype tagging, is formulated as a combinatorial optimization problem and analyzed in terms of complexity and approximation properties. Results It is shown that the tagging problem is NP-hard but approximable within 1 + ln((n2 - n/2 for n haplotypes but not approximable within (1 - ε ln(n/2 for any ε > 0 unless NP ⊂ DTIME(nlog log n. A simple, very easily implementable algorithm that exhibits the above upper bound on solution quality is presented. This algorithm has running time O((2m - p + 1 ≤ O(m(n2 - n/2 where p ≤ min(n, m for n haplotypes of size m. As we show that the approximation bound is asymptotically tight, the algorithm presented is optimal with respect to this asymptotic bound. Conclusion The haplotype tagging problem is hard, but approachable with a fast, practical, and surprisingly simple algorithm that cannot be significantly improved upon on a single processor machine. Hence, significant improvement in computatational efforts expended can only be expected if the computational effort is distributed and done in parallel.

  9. Predicting floods with Flickr tags.

    Science.gov (United States)

    Tkachenko, Nataliya; Jarvis, Stephen; Procter, Rob

    2017-01-01

    Increasingly, user generated content (UGC) in social media postings and their associated metadata such as time and location stamps are being used to provide useful operational information during natural hazard events such as hurricanes, storms and floods. The main advantage of these new sources of data are twofold. First, in a purely additive sense, they can provide much denser geographical coverage of the hazard as compared to traditional sensor networks. Second, they provide what physical sensors are not able to do: By documenting personal observations and experiences, they directly record the impact of a hazard on the human environment. For this reason interpretation of the content (e.g., hashtags, images, text, emojis, etc) and metadata (e.g., keywords, tags, geolocation) have been a focus of much research into social media analytics. However, as choices of semantic tags in the current methods are usually reduced to the exact name or type of the event (e.g., hashtags '#Sandy' or '#flooding'), the main limitation of such approaches remains their mere nowcasting capacity. In this study we make use of polysemous tags of images posted during several recent flood events and demonstrate how such volunteered geographic data can be used to provide early warning of an event before its outbreak.

  10. Generation of longer 3' cDNA fragments from massively parallel signature sequencing tags.

    Science.gov (United States)

    Silva, Ana Paula M; Chen, Jianjun; Carraro, Dirce M; Wang, San Ming; Camargo, Anamaria A

    2004-07-06

    Massively Parallel Signature Sequencing (MPSS) is a powerful technique for genome-wide gene expression analysis, which, similar to SAGE, relies on the production of short tags proximal to the 3'end of transcripts. A single MPSS experiment can generate over 10(7) tags, providing a 10-fold coverage of the transcripts expressed in a human cell. A significant fraction of MPSS tags cannot be assigned to known transcripts (orphan tags) and are likely to be derived from transcripts expressed at very low levels (approximately 1 copy per cell). In order to explore the potential of MPSS for the characterization of the human transcriptome, we have adapted the GLGI protocol (Generation of Longer cDNA fragments from SAGE tags for Gene Identification) to convert MPSS tags into their corresponding 3' cDNA fragments. GLGI-MPSS was applied to 83 orphan tags and 41 cDNA fragments were obtained. The analysis of these 41 fragments allowed the identification of novel transcripts, alternative tags generated from polymorphic and alternatively spliced transcripts, as well as the detection of artefactual MPSS tags. A systematic large-scale analysis of the genome by MPSS, in combination with the use of GLGI-MPSS protocol, will certainly provide a complementary approach to generate the complete catalog of human transcripts.

  11. Generation of longer 3′ cDNA fragments from massively parallel signature sequencing tags

    Science.gov (United States)

    Silva, Ana Paula M.; Chen, Jianjun; Carraro, Dirce M.; Wang, San Ming; Camargo, Anamaria A.

    2004-01-01

    Massively Parallel Signature Sequencing (MPSS) is a powerful technique for genome-wide gene expression analysis, which, similar to SAGE, relies on the production of short tags proximal to the 3′end of transcripts. A single MPSS experiment can generate over 107 tags, providing a 10-fold coverage of the transcripts expressed in a human cell. A significant fraction of MPSS tags cannot be assigned to known transcripts (orphan tags) and are likely to be derived from transcripts expressed at very low levels (∼1 copy per cell). In order to explore the potential of MPSS for the characterization of the human transcriptome, we have adapted the GLGI protocol (Generation of Longer cDNA fragments from SAGE tags for Gene Identification) to convert MPSS tags into their corresponding 3′ cDNA fragments. GLGI-MPSS was applied to 83 orphan tags and 41 cDNA fragments were obtained. The analysis of these 41 fragments allowed the identification of novel transcripts, alternative tags generated from polymorphic and alternatively spliced transcripts, as well as the detection of artefactual MPSS tags. A systematic large-scale analysis of the genome by MPSS, in combination with the use of GLGI-MPSS protocol, will certainly provide a complementary approach to generate the complete catalog of human transcripts. PMID:15247327

  12. Use of an external source (60Co) for 32P detection efficiency determination by the Cerenkov effect, in soil extracts

    International Nuclear Information System (INIS)

    Nascimento Filho, V.F. do.

    1975-03-01

    The detection of 32 P in aqueous extracts is usually made with the aid of a Geiger-Muller detector, with thin window and sample on a planchet. Presently the technique is being developed of detection of high energy beta particles emitters ( 32 P, 42 K, 86 Rb) through the Cerenkov effect, using a commercial liquid scintillation system. This technique, despite being approximately 30 times more sensitive, has the inconvenience of varying the detection efficiency, mainly for color samples (soil extracts, for instance). From this stems the need for determining the detection efficiency for each sample. The internal standardization and channels ratio methods show a series of drawbacks, mainly the non-reutilization of the samples (1st method) and statistical uncertainty for low activity samples (2nd method). The elimination of these dreawbacks can be achieved through the utilization of the external standardization method. A 60 Co source with 1,4 μCi activity has been adapted to the sample elevator of the detector system, and a comparison was made with the channels ratio method to evaluate the efficiency of 32 P detection in soil extracts (P extraction and fractionation). The external standardization method showed to be more accurate, besides being influenced to a lesser degree by high voltage variation, sample volume and vial types. In the case of large samples, it is advisable to carry out detection in vials filled up to their full capacity; in the case of small samples, the whole volume should be transferred to the vials and completed up to 9 ml for nylon vials,10 ml for glass vials and 11 to 14 ml for polyethilene vials. On the other hand, plastic vials showed higher detection efficiency than ones. As to background radiation, the lowest rates were given by nylon vials and the highest by Beckman glass vials [pt

  13. Recovery efficiency and limit of detection of aerosolized Bacillus anthracis Sterne from environmental surface samples.

    Science.gov (United States)

    Estill, Cheryl Fairfield; Baron, Paul A; Beard, Jeremy K; Hein, Misty J; Larsen, Lloyd D; Rose, Laura; Schaefer, Frank W; Noble-Wang, Judith; Hodges, Lisa; Lindquist, H D Alan; Deye, Gregory J; Arduino, Matthew J

    2009-07-01

    After the 2001 anthrax incidents, surface sampling techniques for biological agents were found to be inadequately validated, especially at low surface loadings. We aerosolized Bacillus anthracis Sterne spores within a chamber to achieve very low surface loading (ca. 3, 30, and 200 CFU per 100 cm(2)). Steel and carpet coupons seeded in the chamber were sampled with swab (103 cm(2)) or wipe or vacuum (929 cm(2)) surface sampling methods and analyzed at three laboratories. Agar settle plates (60 cm(2)) were the reference for determining recovery efficiency (RE). The minimum estimated surface concentrations to achieve a 95% response rate based on probit regression were 190, 15, and 44 CFU/100 cm(2) for sampling steel surfaces and 40, 9.2, and 28 CFU/100 cm(2) for sampling carpet surfaces with swab, wipe, and vacuum methods, respectively; however, these results should be cautiously interpreted because of high observed variability. Mean REs at the highest surface loading were 5.0%, 18%, and 3.7% on steel and 12%, 23%, and 4.7% on carpet for the swab, wipe, and vacuum methods, respectively. Precision (coefficient of variation) was poor at the lower surface concentrations but improved with increasing surface concentration. The best precision was obtained with wipe samples on carpet, achieving 38% at the highest surface concentration. The wipe sampling method detected B. anthracis at lower estimated surface concentrations and had higher RE and better precision than the other methods. These results may guide investigators to more meaningfully conduct environmental sampling, quantify contamination levels, and conduct risk assessment for humans.

  14. Gravitational waves from inspiralling compact binaries: Hexagonal template placement and its efficiency in detecting physical signals

    International Nuclear Information System (INIS)

    Cokelaer, T.

    2007-01-01

    Matched filtering is used to search for gravitational waves emitted by inspiralling compact binaries in data from the ground-based interferometers. One of the key aspects of the detection process is the design of a template bank that covers the astrophysically pertinent parameter space. In an earlier paper, we described a template bank that is based on a square lattice. Although robust, we showed that the square placement is overefficient, with the implication that it is computationally more demanding than required. In this paper, we present a template bank based on an hexagonal lattice, which size is reduced by 40% with respect to the proposed square placement. We describe the practical aspects of the hexagonal template bank implementation, its size, and computational cost. We have also performed exhaustive simulations to characterize its efficiency and safeness. We show that the bank is adequate to search for a wide variety of binary systems (primordial black holes, neutron stars, and stellar-mass black holes) and in data from both current detectors (initial LIGO, Virgo and GEO600) as well as future detectors (advanced LIGO and EGO). Remarkably, although our template bank placement uses a metric arising from a particular template family, namely, stationary phase approximation, we show that it can be used successfully with other template families (e.g., Pade resummation and effective one-body approximation). This quality of being effective for different template families makes the proposed bank suitable for a search that would use several of them in parallel (e.g., in a binary black hole search). The hexagonal template bank described in this paper is currently used to search for nonspinning inspiralling compact binaries in data from the Laser Interferometer Gravitational-Wave Observatory (LIGO)

  15. Recovery Efficiency and Limit of Detection of Aerosolized Bacillus anthracis Sterne from Environmental Surface Samples ▿

    Science.gov (United States)

    Estill, Cheryl Fairfield; Baron, Paul A.; Beard, Jeremy K.; Hein, Misty J.; Larsen, Lloyd D.; Rose, Laura; Schaefer, Frank W.; Noble-Wang, Judith; Hodges, Lisa; Lindquist, H. D. Alan; Deye, Gregory J.; Arduino, Matthew J.

    2009-01-01

    After the 2001 anthrax incidents, surface sampling techniques for biological agents were found to be inadequately validated, especially at low surface loadings. We aerosolized Bacillus anthracis Sterne spores within a chamber to achieve very low surface loading (ca. 3, 30, and 200 CFU per 100 cm2). Steel and carpet coupons seeded in the chamber were sampled with swab (103 cm2) or wipe or vacuum (929 cm2) surface sampling methods and analyzed at three laboratories. Agar settle plates (60 cm2) were the reference for determining recovery efficiency (RE). The minimum estimated surface concentrations to achieve a 95% response rate based on probit regression were 190, 15, and 44 CFU/100 cm2 for sampling steel surfaces and 40, 9.2, and 28 CFU/100 cm2 for sampling carpet surfaces with swab, wipe, and vacuum methods, respectively; however, these results should be cautiously interpreted because of high observed variability. Mean REs at the highest surface loading were 5.0%, 18%, and 3.7% on steel and 12%, 23%, and 4.7% on carpet for the swab, wipe, and vacuum methods, respectively. Precision (coefficient of variation) was poor at the lower surface concentrations but improved with increasing surface concentration. The best precision was obtained with wipe samples on carpet, achieving 38% at the highest surface concentration. The wipe sampling method detected B. anthracis at lower estimated surface concentrations and had higher RE and better precision than the other methods. These results may guide investigators to more meaningfully conduct environmental sampling, quantify contamination levels, and conduct risk assessment for humans. PMID:19429546

  16. Behavior Analysis Based on Coordinates of Body Tags

    Science.gov (United States)

    Luštrek, Mitja; Kaluža, Boštjan; Dovgan, Erik; Pogorelc, Bogdan; Gams, Matjaž

    This paper describes fall detection, activity recognition and the detection of anomalous gait in the Confidence project. The project aims to prolong the independence of the elderly by detecting falls and other types of behavior indicating a health problem. The behavior will be analyzed based on the coordinates of tags worn on the body. The coordinates will be detected with radio sensors. We describe two Confidence modules. The first one classifies the user's activity into one of six classes, including falling. The second one detects walking anomalies, such as limping, dizziness and hemiplegia. The walking analysis can automatically adapt to each person by using only the examples of normal walking of that person. Both modules employ machine learning: the paper focuses on the features they use and the effect of tag placement and sensor noise on the classification accuracy. Four tags were enough for activity recognition accuracy of over 93% at moderate sensor noise, while six were needed to detect walking anomalies with the accuracy of over 90%.

  17. Modulation transfer function and detective quantum efficiency of electron bombarded charge coupled device detector for low energy electrons

    Czech Academy of Sciences Publication Activity Database

    Horáček, Miroslav

    2005-01-01

    Roč. 76, č. 9 (2005), 093704:1-6 ISSN 0034-6748 R&D Projects: GA ČR(CZ) GA202/03/1575 Keywords : electron bombarded CCD * modulation transfer function * detective quantum efficiency Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 1.235, year: 2005

  18. Photon detection efficiency of laboratory-based x-ray phase contrast imaging techniques for mammography: a Monte Carlo study

    Science.gov (United States)

    Saghamanesh, S.; Aghamiri, S. M.; Kamali-Asl, A.; Yashiro, W.

    2017-09-01

    An important challenge in real-world biomedical applications of x-ray phase contrast imaging (XPCI) techniques is the efficient use of the photon flux generated by an incoherent and polychromatic x-ray source. This efficiency can directly influence dose and exposure time and ideally should not affect the superior contrast and sensitivity of XPCI. In this paper, we present a quantitative evaluation of the photon detection efficiency of two laboratory-based XPCI methods, grating interferometry (GI) and coded-aperture (CA). We adopt a Monte Carlo approach to simulate existing prototypes of those systems, tailored for mammography applications. Our simulations were validated by means of a simple experiment performed on a CA XPCI system. Our results show that the fraction of detected photons in the standard energy range of mammography are about 1.4% and 10% for the GI and CA techniques, respectively. The simulations indicate that the design of the optical components plays an important role in the higher efficiency of CA compared to the GI method. It is shown that the use of lower absorbing materials as the substrates for GI gratings can improve its flux efficiency by up to four times. Along similar lines, we also show that an optimized and compact configuration of GI could lead to a 3.5 times higher fraction of detected counts compared to a standard and non-optimised GI implementation.

  19. Secure passive RFID tag with seal

    Energy Technology Data Exchange (ETDEWEB)

    Nekoogar, Faranak; Reynolds, Matthew; Lefton, Scott; Dowla, Farid; Twogood, Richard

    2017-11-14

    A secure passive RFID tag system comprises at least one base station and at least one passive RFID tag. The tag includes a fiber optic cable with the cable ends sealed within the tag and the middle portion forming an external loop. The loop may be secured to at least portions of an object. The tag transmits and receives an optical signal through the fiber optic cable, and the cable is configured to be damaged or broken in response to removal or tampering attempts, wherein the optical signal is significantly altered if the cable is damaged or broken. The tag transmits the optical signal in response to receiving a radio signal from the base station and compares the transmitted optical signal to the received optical signal. If the transmitted optical signal and the received optical signal are identical, the tag transmits an affirmative radio signal to the base station.

  20. Specifically and wash-free labeling of SNAP-tag fused proteins with a hybrid sensor to monitor local micro-viscosity.

    Science.gov (United States)

    Wang, Chao; Song, Xinbo; Chen, Lingcheng; Xiao, Yi

    2017-05-15

    Viscosity, as one of the major factors of intracellular microenvironment, influences the function of proteins. To detect local micro-viscosity of a protein, it is a precondition to apply a viscosity sensor for specifically target to proteins. However, all the reported small-molecule probes are just suitable for sensing/imaging of macro-viscosity in biological fluids of entire cells or organelles. To this end, we developed a hybrid sensor BDP-V BG by connecting a viscosity-sensitive boron-dipyrromethene (BODIPY) molecular rotor (BDP-V) to O 6 -benzylguanine (BG) for specific detection of local micro-viscosity of SNAP-tag fused proteins. We measured and calculated the reaction efficiency between the sensor and SNAP-tag protein in vitro to confirm the high labeling specificity. We also found that the labeling reaction results in a 53-fold fluorescence enhancement for the rotor, which qualifies it as a wash-free sensor with ignorable background fluorescence. The high sensitivity of protein labeled sensor (BDP-V-SNAP) to the changes of local viscosity was evaluated by detecting the enhancement of fluorescence lifetimes. Further, with the sensor BDP-V BG, we achieved high specific labeling of cells expressing two SNAP-tag fused proteins (nuclear histone H2B and mitochondrial COX8A). Two-photon excited fluorescence lifetime imaging revealed that, the micro-viscosities nearby the SNAP-tag fused two proteins are distinct. The different changes of local micro-viscosity of SNAP-tag fused histone protein in apoptosis induced by three nucleus-targeted drugs were also characterized for the first time. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. Study of an efficient application of the tagged bremsstrahlung in double-polarization experiments in the GeV range and the use of the inelastic electron scattering under extremely forward angles as alternative to the tagged bremsstrahlung; Studie eines effizienten Einsatzes der markierten Bremsstrahlung bei Doppelpolarisationsexperimenten im GeV-Bereich und der Nutzung der inelastischen Elektronstreuung unter extremen Vorwaertswinkeln als Alternative zur markierten Bremsstrahlung

    Energy Technology Data Exchange (ETDEWEB)

    Konrad, M.

    2006-03-15

    For the preparation of photonic probes for hadron physics the determination of energy and polarization of the photons is essential. In this dissertation in a first part a possibility of the determination of the degree of polarization by use of the asymmetry observables is presented. In a second part a possibility isd discussed to perform an energy and polarization tagging of nearly real photons in electron scattering under small Q{sup 2}. By this method it should be possible to tag billions of photons per second.

  2. Beta particle detection efficiency of the radiation sensor made from a mixture of polyaniline and titanium oxide

    Energy Technology Data Exchange (ETDEWEB)

    Tamura, M., E-mail: tamuram@hep.sc.niigata-u.ac.jp [Graduate School of Science and Technology, Niigata University, Niigata 950-2181 (Japan); Carlit Holdings Co., Ltd., Tokyo 104-0031 (Japan); Miyata, H., E-mail: miyata@hep.sc.niigata-u.ac.jp [Graduate School of Science and Technology, Niigata University, Niigata 950-2181 (Japan); Department of Physics, Niigata University, Niigata 950-2181 (Japan); Katsumata, M. [Department of Physics, Niigata University, Niigata 950-2181 (Japan); Matsuda, K.; Ueno, T.; Ito, D. [Graduate School of Science and Technology, Niigata University, Niigata 950-2181 (Japan); Suzuki, T. [Carlit Holdings Co., Ltd., Tokyo 104-0031 (Japan)

    2016-08-21

    We developed a new real-time radiation sensor using an organic semiconductor and measured its β-particle detection sensitivity. This sensor is fabricated by simply combining a p-type semiconductor, polyaniline (Pani), with an n-type semiconductor, TiO{sub 2}, and processing the compound. Since Pani and TiO{sub 2} are both inexpensive materials, the sensor can be fabricated at a lower cost than inorganic semiconductor sensors. The signal of each fabricated sensor was measured by a charge sensitive ADC for the irradiation of β-particles. The response signal data of the ADC for each irradiation was measured to calculate the detection efficiency of the detector. The maximum detection efficiency measured as β-particle sensitivity of the sensor was 1%. This β-particle sensitivity is higher than that reported of Pani sensors in the past.

  3. External tagging does not affect the feeding behavior of a coral reef fish, Chaetodon vagabundus (Pisces: Chaetodontidae)

    KAUST Repository

    Berumen, Michael L.

    2009-11-10

    Increasingly, the ability to recognize individual fishes is important for studies of population dynamics, ecology, and behavior. Although a variety of methods exist, external tags remain one of the most widely applied because they are both effective and cost efficient. However, a key assumption is that neither the tagging procedure nor the presence of a tag negatively affects the individual. While this has been demonstrated for relatively coarse metrics such as growth and survival, few studies have examined the impact of tags and tagging on more subtle aspects of behavior. We tagged adult vagabond butterflyfish (Chaetodon vagabundus) occupying a 30-ha insular reef in Kimbe Bay, Papua New Guinea, using a commonly-utilized t-bar anchor tag. We quantified and compared feeding behavior (bite rate), which is sensitive to stress, of tagged and untagged individuals over four separate sampling periods spanning 4 months post-tagging. Bite rates did not differ between tagged and untagged individuals at each sampling period and, combined with additional anecdotal observations of normal pairing behavior and successful reproduction, suggest that tagging did not adversely affect individuals. © Springer Science+Business Media B.V. 2009.

  4. An oligonucleotide-tagged microarray for routine diagnostics of colon cancer by genotyping KRAS mutations

    DEFF Research Database (Denmark)

    Liu, Yuliang; Guðnason, Haukur; Li, Yiping

    2014-01-01

    mutations at codon 12 of KRAS derived from cancer cells and clinical samples could be unambiguously detected. KRAS mutations were accurately detected when the mutant DNA was present only in 10% of the starting mixed materials including wild-type genomic DNA, which was isolated from either cancer cells...... or spiked fecal samples. The immobilized tag-probes were stable under multiple thermal cycling treatments, allowing re-use of the tag-microarray and further optimization to solid PCR. Our results demonstrated that a novel oligonucleotide-tagged microarray system has been developed which would be suitable...

  5. Directly Printable Organic ASK Based Chipless RFID Tag for IoT Applications

    Directory of Open Access Journals (Sweden)

    A. Habib

    2017-06-01

    Full Text Available A chipless RFID tag with unique ASK encoding technique is presented in this paper. The coding efficiency is enhanced regarding tag capacity. The amplitude varia¬tions of the backscattered RFID signal is used for encoding data instead of OOK. Strips of different widths are used to have amplitude variations. The ASK technique is applied using three different substrates of Kapton®HN, PET, and paper. To incorporate ASK technique, dual polarized rhombic shaped resonators are designed. These tags operate in the frequency range of 3.1–10.6 GHz with size of 70 × 42 mm^2. The presented tags are flexible and offer easy printability. The paper-based decomposable organic tag appears as an ultra low-cost solution for wide scale tracking. This feature enables them to secure a prominent position in the emerging fields of IoT and green electronics.

  6. Evaluation of species-dependent detection efficiencies in the aerosol mass spectrometer

    Science.gov (United States)

    Mass concentrations of chemical species calculated from the aerosol mass spectrometer (AMS) depend on two factors: particle collection efficiency (CE) and relative ionization efficiency (RIE, relative to the primary calibrant ammonium nitrate). While previous studies have characterized CE, RIE is re...

  7. Highly selective end-tagged antimicrobial peptides derived from PRELP.

    Directory of Open Access Journals (Sweden)

    Martin Malmsten

    Full Text Available BACKGROUND: Antimicrobial peptides (AMPs are receiving increasing attention due to resistance development against conventional antibiotics. Pseudomonas aeruginosa and Staphylococcus aureus are two major pathogens involved in an array of infections such as ocular infections, cystic fibrosis, wound and post-surgery infections, and sepsis. The goal of the study was to design novel AMPs against these pathogens. METHODOLOGY AND PRINCIPAL FINDINGS: Antibacterial activity was determined by radial diffusion, viable count, and minimal inhibitory concentration assays, while toxicity was evaluated by hemolysis and effects on human epithelial cells. Liposome and fluorescence studies provided mechanistic information. Protease sensitivity was evaluated after subjection to human leukocyte elastase, staphylococcal aureolysin and V8 proteinase, as well as P. aeruginosa elastase. Highly active peptides were evaluated in ex vivo skin infection models. C-terminal end-tagging by W and F amino acid residues increased antimicrobial potency of the peptide sequences GRRPRPRPRP and RRPRPRPRP, derived from proline arginine-rich and leucine-rich repeat protein (PRELP. The optimized peptides were antimicrobial against a range of gram-positive S. aureus and gram-negative P. aeruginosa clinical isolates, also in the presence of human plasma and blood. Simultaneously, they showed low toxicity against mammalian cells. Particularly W-tagged peptides displayed stability against P. aeruginosa elastase, and S. aureus V8 proteinase and aureolysin, and the peptide RRPRPRPRPWWWW-NH(2 was effective against various "superbugs" including vancomycin-resistant enterococci, multi-drug resistant P. aeruginosa, and methicillin-resistant S. aureus, as well as demonstrated efficiency in an ex vivo skin wound model of S. aureus and P. aeruginosa infection. CONCLUSIONS/SIGNIFICANCE: Hydrophobic C-terminal end-tagging of the cationic sequence RRPRPRPRP generates highly selective AMPs with potent

  8. Dogs can detect scat samples more efficiently than humans: an experiment in a continuous Atlantic Forest remnant

    Directory of Open Access Journals (Sweden)

    Márcio L. de Oliveira

    2012-04-01

    Full Text Available Scat-detection dogs have been used to locate feces of rare and elusive species across tropical biomes. However their detection efficiency in relation to human observers has rarely been evaluated. In this study, we evaluated the ability of a scat detection dog to locate feces in comparison with human researchers. Human researchers and a scat detection dog surveyed for deer (Mazama spp. feces in dense ombrofilous Atlantic forest in the Paranapiacaba continuum, SP, Brazil. A controlled experiment was used to assess the maximum effective perpendicular distance from a transect search line that the dog could detect a Mazama spp fecal sample. Results from a linear regression model revealed that the maximum effective perpendicular distance from a transect search line that the dog could detect a scat was 7.2 m. The detection success from our surveys in the Atlantic forest was zero for humans and 0.15 samples/ha or 0.20 samples/km walked for the dog team. Our results demonstrated the importance of scat-detection dogs for non invasive sampling and provide data relevant for the design of future studies.

  9. Direct determination of recoil ion detection efficiency for coincidence time-of-flight studies of molecular fragmentation

    Science.gov (United States)

    Ben-Itzhak, I.; Carnes, K. D.; Ginther, S. G.; Johnson, D. T.; Norris, P. J.; Weaver, O. L.

    1993-06-01

    Molecular fragmentation of diatomic and small polyatomic molecules caused by fast ion impact has been studied. The evaluation of the cross sections of the different fragmentation channels depends strongly on the recoil ion detection efficiency, ɛ r (single ions proportional to ɛ r, and ion pairs to ɛ r2, etc.). A method is suggested for the direct determination of this detection efficiency. This method is based on the fact that fast H + + CH 4 collisions produce C 2+ fragments only in coincidence with H + and H +2 fragments, that is, there is a negligible number of C 2+ singles, if any. The measured yield of C 2+ singles is therefore due to events in which the H +m of the H +m +C 2+ ion pair was not detected and thus is proportional to 1-ɛ r. Methane fragmentation caused by 1 MeV proton impact is used to evaluate directly the recoil ion detection efficiency and to demonstrate the method of deriving the cross sections of all breakup channels.

  10. Gene Tagging Strategies To Assess Protein Expression, Localization, and Function in Drosophila

    Science.gov (United States)

    Kanca, Oguz; Bellen, Hugo J.; Schnorrer, Frank

    2017-01-01

    Analysis of gene function in complex organisms relies extensively on tools to detect the cellular and subcellular localization of gene products, especially proteins. Typically, immunostaining with antibodies provides these data. However, due to cost, time, and labor limitations, generating specific antibodies against all proteins of a complex organism is not feasible. Furthermore, antibodies do not enable live imaging studies of protein dynamics. Hence, tagging genes with standardized immunoepitopes or fluorescent tags that permit live imaging has become popular. Importantly, tagging genes present in large genomic clones or at their endogenous locus often reports proper expression, subcellular localization, and dynamics of the encoded protein. Moreover, these tagging approaches allow the generation of elegant protein removal strategies, standardization of visualization protocols, and permit protein interaction studies using mass spectrometry. Here, we summarize available genomic resources and techniques to tag genes and discuss relevant applications that are rarely, if at all, possible with antibodies. PMID:28978772

  11. Preliminary evaluation of a fluorescence and radioisotope nuclear smuggling deterrence tag - final report (IL500E)

    International Nuclear Information System (INIS)

    Hartenstein, S.D.; Aryaeinejad, R.; Delmastro, J.R.

    1997-04-01

    This report summarizes the efforts completed in identifying candidate fluorescence compounds and radioisotopes for a developing tagging system. The tagging system is being developed as a deterrent to nuclear smuggling, by providing a means of: (1) tracing materials and pilferers to the facility of origin for any recovered special nuclear materials, (2) inventory control of long-term stored items containing special nuclear materials, and (3) tracking materials transferred between facilities. The system uses three types of materials to cover a range of applications intended to prevent the pilfering of special nuclear materials. One material, fluorescent compounds which are invisible without ultraviolet or near-infrared detection systems, is marked on controlled items with a tracking pattern that corresponds to a specified item in a specified location in the data control system. The tagging system uses an invisible, fluorescent dusting powder to mark equipment and personnel who inappropriately handle the tagged material. The tagging system also uses unique combinations of radionuclides to identify the facility of origin for any special nuclear material. This report also summarizes the efforts completed in identifying hardware that will be used for the tagging system. This hardware includes the devices for applying the tagging materials, the commercially available fluorescence detection systems, and gamma ray detection systems assembled from existing, commercially available technologies

  12. Extending birthday paradox theory to estimate the number of tags in RFID systems.

    Directory of Open Access Journals (Sweden)

    Masoud Shakiba

    Full Text Available The main objective of Radio Frequency Identification systems is to provide fast identification for tagged objects. However, there is always a chance of collision, when tags transmit their data to the reader simultaneously. Collision is a time-consuming event that reduces the performance of RFID systems. Consequently, several anti-collision algorithms have been proposed in the literature. Dynamic Framed Slotted ALOHA (DFSA is one of the most popular of these algorithms. DFSA dynamically modifies the frame size based on the number of tags. Since the real number of tags is unknown, it needs to be estimated. Therefore, an accurate tag estimation method has an important role in increasing the efficiency and overall performance of the tag identification process. In this paper, we propose a novel estimation technique for DFSA anti-collision algorithms that applies birthday paradox theory to estimate the number of tags accurately. The analytical discussion and simulation results prove that the proposed method increases the accuracy of tag estimation and, consequently, outperforms previous schemes.

  13. Extending birthday paradox theory to estimate the number of tags in RFID systems.

    Science.gov (United States)

    Shakiba, Masoud; Singh, Mandeep Jit; Sundararajan, Elankovan; Zavvari, Azam; Islam, Mohammad Tariqul

    2014-01-01

    The main objective of Radio Frequency Identification systems is to provide fast identification for tagged objects. However, there is always a chance of collision, when tags transmit their data to the reader simultaneously. Collision is a time-consuming event that reduces the performance of RFID systems. Consequently, several anti-collision algorithms have been proposed in the literature. Dynamic Framed Slotted ALOHA (DFSA) is one of the most popular of these algorithms. DFSA dynamically modifies the frame size based on the number of tags. Since the real number of tags is unknown, it needs to be estimated. Therefore, an accurate tag estimation method has an important role in increasing the efficiency and overall performance of the tag identification process. In this paper, we propose a novel estimation technique for DFSA anti-collision algorithms that applies birthday paradox theory to estimate the number of tags accurately. The analytical discussion and simulation results prove that the proposed method increases the accuracy of tag estimation and, consequently, outperforms previous schemes.

  14. Detecting and correcting partial errors: Evidence for efficient control without conscious access.

    Science.gov (United States)

    Rochet, N; Spieser, L; Casini, L; Hasbroucq, T; Burle, B

    2014-09-01

    Appropriate reactions to erroneous actions are essential to keeping behavior adaptive. Erring, however, is not an all-or-none process: electromyographic (EMG) recordings of the responding muscles have revealed that covert incorrect response activations (termed "partial errors") occur on a proportion of overtly correct trials. The occurrence of such "partial errors" shows that incorrect response activations could be corrected online, before turning into overt errors. In the present study, we showed that, unlike overt errors, such "partial errors" are poorly consciously detected by participants, who could report only one third of their partial errors. Two parameters of the partial errors were found to predict detection: the surface of the incorrect EMG burst (larger for detected) and the correction time (between the incorrect and correct EMG onsets; longer for detected). These two parameters provided independent information. The correct(ive) responses associated with detected partial errors were larger than the "pure-correct" ones, and this increase was likely a consequence, rather than a cause, of the detection. The respective impacts of the two parameters predicting detection (incorrect surface and correction time), along with the underlying physiological processes subtending partial-error detection, are discussed.

  15. Evaluation of carbon nanotube based copper nanoparticle composite for the efficient detection of agroviruses

    Science.gov (United States)

    Nanomaterials based sensors offer sensitivity and selectivity for the detection of a specific analyte-of-the-interest. Described here is a novel assay for the detection of a DNA sequence based on nanostructured carbon nanotubes/copper nanoparticles composite. This assay was modeled on strong electro...

  16. Improved High Efficiency MCPs for Detection of Photons and Large Biomolecules Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This SBIR Phase I proposes to investigate the use of proprietary coating materials to enhance the sensitivity, efficiency, and lifetime of microchannel plate (MCP)...

  17. Design of a Humidity Sensor Tag for Passive Wireless Applications.

    Science.gov (United States)

    Wu, Xiang; Deng, Fangming; Hao, Yong; Fu, Zhihui; Zhang, Lihua

    2015-10-07

    This paper presents a wireless humidity sensor tag for low-cost and low-power applications. The proposed humidity sensor tag, based on radio frequency identification (RFID) technology, was fabricated in a standard 0.18 μm complementary metal oxide semiconductor (CMOS) process. The top metal layer was deposited to form the interdigitated electrodes, which were then filled with polyimide as the humidity sensing layer. A two-stage rectifier adopts a dynamic bias-voltage generator to boost the effective gate-source voltage of the switches in differential-drive architecture, resulting in a flat power conversion efficiency curve. The capacitive sensor interface, based on phase-locked loop (PLL) theory, employs a simple architecture and can work with 0.5 V supply voltage. The measurement results show that humidity sensor tag achieves excellent linearity, hysteresis and stability performance. The total power-dissipation of the sensor tag is 2.5 μW, resulting in a maximum operating distance of 23 m under 4 W of radiation power of the RFID reader.

  18. Job optimization in ATLAS TAG-based distributed analysis

    Science.gov (United States)

    Mambelli, M.; Cranshaw, J.; Gardner, R.; Maeno, T.; Malon, D.; Novak, M.

    2010-04-01

    The ATLAS experiment is projected to collect over one billion events/year during the first few years of operation. The efficient selection of events for various physics analyses across all appropriate samples presents a significant technical challenge. ATLAS computing infrastructure leverages the Grid to tackle the analysis across large samples by organizing data into a hierarchical structure and exploiting distributed computing to churn through the computations. This includes events at different stages of processing: RAW, ESD (Event Summary Data), AOD (Analysis Object Data), DPD (Derived Physics Data). Event Level Metadata Tags (TAGs) contain information about each event stored using multiple technologies accessible by POOL and various web services. This allows users to apply selection cuts on quantities of interest across the entire sample to compile a subset of events that are appropriate for their analysis. This paper describes new methods for organizing jobs using the TAGs criteria to analyze ATLAS data. It further compares different access patterns to the event data and explores ways to partition the workload for event selection and analysis. Here analysis is defined as a broader set of event processing tasks including event selection and reduction operations ("skimming", "slimming" and "thinning") as well as DPD making. Specifically it compares analysis with direct access to the events (AOD and ESD data) to access mediated by different TAG-based event selections. We then compare different ways of splitting the processing to maximize performance.

  19. An efficient flow-based botnet detection using supervised machine learning

    DEFF Research Database (Denmark)

    Stevanovic, Matija; Pedersen, Jens Myrup

    2014-01-01

    Botnet detection represents one of the most crucial prerequisites of successful botnet neutralization. This paper explores how accurate and timely detection can be achieved by using supervised machine learning as the tool of inferring about malicious botnet traffic. In order to do so, the paper...... introduces a novel flow-based detection system that relies on supervised machine learning for identifying botnet network traffic. For use in the system we consider eight highly regarded machine learning algorithms, indicating the best performing one. Furthermore, the paper evaluates how much traffic needs...... to accurately and timely detect botnet traffic using purely flow-based traffic analysis and supervised machine learning. Additionally, the results show that in order to achieve accurate detection traffic flows need to be monitored for only a limited time period and number of packets per flow. This indicates...

  20. B-tagging and the search for neutral supersymmetric Higgs bosons at D0

    Energy Technology Data Exchange (ETDEWEB)

    Scanlon, Tim [Imperial College, London (United Kingdom)

    2006-10-01

    A search for neutral supersymmetric Higgs bosons and work relating to the improvement of the b-tagging and trigger capabilities at the D0 detector during Run II of the Fermilab Tevatron collider is presented. The search for evidence of the Higgs sector in the Standard Model (SM) and supersymmetric extensions of the SM are a high priority for the D0 collaboration, and b-tagging and good triggers are a vital component of these searches. The development and commissioning of the first triggers at D0 which use b-tagging is outlined, along with the development of a new secondary vertex b-tagging tool for use in the Level 3 trigger. Upgrades to the Level 3 trigger hit finding code, which have led to significant improvements in the quality and efficiency of the tracking code, and by extension the b-tagging tools, are also presented. An offline Neural Network (NN) b-tagging tool was developed, trained on Monte Carlo and extensively tested and measured on data. The new b-tagging tool significantly improves the b-tagging performance at D0, for a fixed fake rate relative improvements in signal efficiency range from ~ 40% to ~ 15%. Fake rates, for a fixed signal efficiency, are typically reduced to between a quarter and a third of their value. Finally, three versions of the search for neutral supersymmetric Higgs bosons are presented. The latest version of the analysis makes use of almost 1 fb-1 of data, the new NN b-tagger and the new b-tagging triggers, and has set one of the world's best limits on the supersymmetric parameter tanβ in the mass range 90 to 150 GeV.

  1. B-tagging and the search for neutral supersymmetric Higgs bosons at D0

    International Nuclear Information System (INIS)

    Scanlon, Tim; Imperial Coll., London

    2006-01-01

    A search for neutral supersymmetric Higgs bosons and work relating to the improvement of the b-tagging and trigger capabilities at the D0 detector during Run II of the Fermilab Tevatron collider is presented. The search for evidence of the Higgs sector in the Standard Model (SM) and supersymmetric extensions of the SM are a high priority for the D0 collaboration, and b-tagging and good triggers are a vital component of these searches. The development and commissioning of the first triggers at D0 which use b-tagging is outlined, along with the development of a new secondary vertex b-tagging tool for use in the Level 3 trigger. Upgrades to the Level 3 trigger hit finding code, which have led to significant improvements in the quality and efficiency of the tracking code, and by extension the b-tagging tools, are also presented. An offline Neural Network (NN) b-tagging tool was developed, trained on Monte Carlo and extensively tested and measured on data. The new b-tagging tool significantly improves the b-tagging performance at D0, for a fixed fake rate relative improvements in signal efficiency range from ∼ 40% to ∼ 15%. Fake rates, for a fixed signal efficiency, are typically reduced to between a quarter and a third of their value. Finally, three versions of the search for neutral supersymmetric Higgs bosons are presented. The latest version of the analysis makes use of almost 1 fb -1 of data, the new NN b-tagger and the new b-tagging triggers, and has set one of the world's best limits on the supersymmetric parameter tanβ in the mass range 90 to 150 GeV

  2. Tag-gas encapsulated assembly

    International Nuclear Information System (INIS)

    Murabayashi, Hideki.

    1980-01-01

    Purpose: To ensure tag-gas release upon failure of a fuel cladding tube with no effects from viscosity and surface tension of low melting alloy, by engaging a closing valve body to the inside surface of an opening of the assembly and securing it by means of a low melting alloy. Constitution: A tag-gas capsule is fabricated from a hollow cylindrical body having an outer diameter corresponding to the inner diameter of a fuel pin cladding tube and welded with an upper end plug and a lower end plug. The upper end plug is formed with an opening of a small diameter at the center and with a concaved face at the inside surface. A valve body of a shape just fitting to the concaved face is engaged and secured by means of a low melting alloy to the inside surface of the upper end plug. When the temperature of the capsule arrives at a predetermined temperature to fuse the alloy, the self weight of the valve body overcomes the viscosity and the surface tension of the alloy and the valve body falls instantly. (Sekiya, K.)

  3. Learner Corpora without Error Tagging

    Directory of Open Access Journals (Sweden)

    Rastelli, Stefano

    2009-01-01

    Full Text Available The article explores the possibility of adopting a form-to-function perspective when annotating learner corpora in order to get deeper insights about systematic features of interlanguage. A split between forms and functions (or categories is desirable in order to avoid the "comparative fallacy" and because – especially in basic varieties – forms may precede functions (e.g., what resembles to a "noun" might have a different function or a function may show up in unexpected forms. In the computer-aided error analysis tradition, all items produced by learners are traced to a grid of error tags which is based on the categories of the target language. Differently, we believe it is possible to record and make retrievable both words and sequence of characters independently from their functional-grammatical label in the target language. For this purpose at the University of Pavia we adapted a probabilistic POS tagger designed for L1 on L2 data. Despite the criticism that this operation can raise, we found that it is better to work with "virtual categories" rather than with errors. The article outlines the theoretical background of the project and shows some examples in which some potential of SLA-oriented (non error-based tagging will be possibly made clearer.

  4. Energy calibration of the photon tagging system

    International Nuclear Information System (INIS)

    Endo, Satoru; Niki, Kazuaki; Suzuki, Akira

    1989-07-01

    There is a photon tagging system at the 1.3 GeV Electron Synchrotron (ES), and the system is designed to produce a monochromatic photon beam with an energy resolution of ±5 MeV. To calibrate the energies of tagged photons produced by this system, electron beams of various energies from ES are injected directly into this system. As the result of this calibration, we conclude that the photon tagging system has the performance of designed values. (author)

  5. B-tagging in CMS at LHC

    CERN Document Server

    Cucciarelli, S

    2003-01-01

    This report provides a review of the main algorithms for offline inclusive b-tagging developed within the CMS community. Two b-tag algorithms, one based on the impact parameter measurement and the other based on the secondary vertices are discussed. The performance of these algorithms are presented for several jet transverse energies and pseudorapidity regions. An additional decay length based b-tag is also described and its preliminary performance is presented. (4 refs) .

  6. Preliminary Assessment of Detection Efficiency for the Geostationary Lightning Mapper Using Intercomparisons with Ground-Based Systems

    Science.gov (United States)

    Bateman, Monte; Mach, Douglas; Blakeslee, Richard J.; Koshak, William

    2018-01-01

    As part of the calibration/validation (cal/val) effort for the Geostationary Lightning Mapper (GLM) on GOES-16, we need to assess instrument performance (detection efficiency and accuracy). One major effort is to calculate the detection efficiency of GLM by comparing to multiple ground-based systems. These comparisons will be done pair-wise between GLM and each other source. A complication in this process is that the ground-based systems sense different properties of the lightning signal than does GLM (e.g., RF vs. optical). Also, each system has a different time and space resolution and accuracy. Preliminary results indicate that GLM is performing at or above its specification.

  7. BlueDetect: An iBeacon-Enabled Scheme for Accurate and Energy-Efficient Indoor-Outdoor Detection and Seamless Location-Based Service

    Directory of Open Access Journals (Sweden)

    Han Zou

    2016-02-01

    Full Text Available The location and contextual status (indoor or outdoor is fundamental and critical information for upper-layer applications, such as activity recognition and location-based services (LBS for individuals. In addition, optimizations of building management systems (BMS, such as the pre-cooling or heating process of the air-conditioning system according to the human traffic entering or exiting a building, can utilize the information, as well. The emerging mobile devices, which are equipped with various sensors, become a feasible and flexible platform to perform indoor-outdoor (IO detection. However, power-hungry sensors, such as GPS and WiFi, should be used with caution due to the constrained battery storage on mobile device. We propose BlueDetect: an accurate, fast response and energy-efficient scheme for IO detection and seamless LBS running on the mobile device based on the emerging low-power iBeacon technology. By leveraging the on-broad Bluetooth module and our proposed algorithms, BlueDetect provides a precise IO detection service that can turn on/off on-board power-hungry sensors smartly and automatically, optimize their performances and reduce the power consumption of mobile devices simultaneously. Moreover, seamless positioning and navigation services can be realized by it, especially in a semi-outdoor environment, which cannot be achieved by GPS or an indoor positioning system (IPS easily. We prototype BlueDetect on Android mobile devices and evaluate its performance comprehensively. The experimental results have validated the superiority of BlueDetect in terms of IO detection accuracy, localization accuracy and energy consumption.

  8. BlueDetect: An iBeacon-Enabled Scheme for Accurate and Energy-Efficient Indoor-Outdoor Detection and Seamless Location-Based Service.

    Science.gov (United States)

    Zou, Han; Jiang, Hao; Luo, Yiwen; Zhu, Jianjie; Lu, Xiaoxuan; Xie, Lihua

    2016-02-22

    The location and contextual status (indoor or outdoor) is fundamental and critical information for upper-layer applications, such as activity recognition and location-based services (LBS) for individuals. In addition, optimizations of building management systems (BMS), such as the pre-cooling or heating process of the air-conditioning system according to the human traffic entering or exiting a building, can utilize the information, as well. The emerging mobile devices, which are equipped with various sensors, become a feasible and flexible platform to perform indoor-outdoor (IO) detection. However, power-hungry sensors, such as GPS and WiFi, should be used with caution due to the constrained battery storage on mobile device. We propose BlueDetect: an accurate, fast response and energy-efficient scheme for IO detection and seamless LBS running on the mobile device based on the emerging low-power iBeacon technology. By leveraging the on-broad Bluetooth module and our proposed algorithms, BlueDetect provides a precise IO detection service that can turn on/off on-board power-hungry sensors smartly and automatically, optimize their performances and reduce the power consumption of mobile devices simultaneously. Moreover, seamless positioning and navigation services can be realized by it, especially in a semi-outdoor environment, which cannot be achieved by GPS or an indoor positioning system (IPS) easily. We prototype BlueDetect on Android mobile devices and evaluate its performance comprehensively. The experimental results have validated the superiority of BlueDetect in terms of IO detection accuracy, localization accuracy and energy consumption.

  9. Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase.

    Science.gov (United States)

    Yin, Jun; Straight, Paul D; McLoughlin, Shaun M; Zhou, Zhe; Lin, Alison J; Golan, David E; Kelleher, Neil L; Kolter, Roberto; Walsh, Christopher T

    2005-11-01

    An 11-residue peptide with the sequence DSLEFIASKLA was identified from a genomic library of Bacillus subtilis by phage display as an efficient substrate for Sfp phosphopantetheinyl transferase-catalyzed protein labeling by small molecule-CoA conjugates. We name this peptide the "ybbR tag," because part of its sequence is derived from the ybbR ORF in the B. subtilis genome. The site of Sfp-catalyzed ybbR tag labeling was mapped to the underlined Ser residue, and the ybbR tag was found to have a strong tendency for adopting an alpha-helical conformation in solution. Here we demonstrate that the ybbR tag can be fused to the N or C termini of target proteins or inserted in a flexible loop in the middle of a target protein for site-specific protein labeling by Sfp. The short size of the ybbR tag and its compatibility with various target proteins, the broad substrate specificity of Sfp for labeling the ybbR tag with small-molecule probes of diverse structures, and the high specificity and efficiency of the labeling reaction make Sfp-catalyzed ybbR tag labeling an attractive tool for expanding protein structural and functional diversities by posttranslational modification.

  10. CT colonography with rectal iodine tagging: Feasibility and comparison with oral tagging in a colorectal cancer screening population

    Energy Technology Data Exchange (ETDEWEB)

    Neri, Emanuele, E-mail: emanuele.neri@med.unipi.it [Diagnostic and Interventional Radiology – Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa (Italy); Mantarro, Annalisa; Faggioni, Lorenzo; Scalise, Paola; Bemi, Pietro; Pancrazi, Francesca [Diagnostic and Interventional Radiology – Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa (Italy); D’Ippolito, Giuseppe [Federal University of São Paulo – Sena Madureira 1500 – Vila Mariana, UNIFESP, São Paulo, SP (Brazil); Bartolozzi, Carlo [Diagnostic and Interventional Radiology – Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa (Italy)

    2015-09-15

    %} 95.3 ÷ 99.8%) vs 97.2% (CI{sub 95%} 89.4 ÷ 99.5%), respectively (p > 0.05). Polyp detection rates were not statistically different between groups 1 and 2 (p > 0.05). Overall examination time was significantly shorter with rectal than with oral tagging (18.3 ± 3.5 vs 215.6 ± 10.3 minutes, respectively; p < 0.0001). Conclusions: Rectal iodine tagging can be an effective alternative to oral tagging for CTC with the advantages of greater patient acceptance and lower overall examination time.

  11. CT colonography with rectal iodine tagging: Feasibility and comparison with oral tagging in a colorectal cancer screening population

    International Nuclear Information System (INIS)

    Neri, Emanuele; Mantarro, Annalisa; Faggioni, Lorenzo; Scalise, Paola; Bemi, Pietro; Pancrazi, Francesca; D’Ippolito, Giuseppe; Bartolozzi, Carlo

    2015-01-01

    .4 ÷ 99.5%), respectively (p > 0.05). Polyp detection rates were not statistically different between groups 1 and 2 (p > 0.05). Overall examination time was significantly shorter with rectal than with oral tagging (18.3 ± 3.5 vs 215.6 ± 10.3 minutes, respectively; p < 0.0001). Conclusions: Rectal iodine tagging can be an effective alternative to oral tagging for CTC with the advantages of greater patient acceptance and lower overall examination time

  12. The Efficiency of Operating Microscope Compared with Unaided Visual Examination, Conventional and Digital Intraoral Radiography for Proximal Caries Detection

    OpenAIRE

    Peker, Ilkay; Toraman Alkurt, Meryem; Bala, Oya; Altunkaynak, Bulent

    2009-01-01

    Objective. The purpose of this study was to evaluate the efficiency of operating microscope compared with unaided visual examination, conventional and digital intraoral radiography for proximal caries detection. Materials and Methods. The study was based on 48 extracted human posterior permanent teeth. The teeth were examined with unaided visual examination, operating microscope, conventional bitewing and digital intraoral radiographs. Then, true caries depth was determined by histological ex...

  13. Graph based techniques for tag cloud generation

    DEFF Research Database (Denmark)

    Leginus, Martin; Dolog, Peter; Lage, Ricardo Gomes

    2013-01-01

    Tag cloud is one of the navigation aids for exploring documents. Tag cloud also link documents through the user defined terms. We explore various graph based techniques to improve the tag cloud generation. Moreover, we introduce relevance measures based on underlying data such as ratings...... or citation counts for improved measurement of relevance of tag clouds. We show, that on the given data sets, our approach outperforms the state of the art baseline methods with respect to such relevance by 41 % on Movielens dataset and by 11 % on Bibsonomy data set....

  14. Using Interference to Block RFID Tags

    DEFF Research Database (Denmark)

    Krigslund, Rasmus; Popovski, Petar; Pedersen, Gert Frølund

    We propose a novel method to block RFID tags from responding, using intentional interference. We focus on the experimental evaluation, where we impose interference on the download and uplink, respectively. The results are positive, where modulated CCI shows most effective to block a tag.......We propose a novel method to block RFID tags from responding, using intentional interference. We focus on the experimental evaluation, where we impose interference on the download and uplink, respectively. The results are positive, where modulated CCI shows most effective to block a tag....

  15. Discharge residence of TLD tagged fish

    International Nuclear Information System (INIS)

    Romberg, G.P.; Prepejchal, W.

    1974-01-01

    Although visual observations suggested that fish remained in the discharge for considerable periods, temperature-sensitive tags indicated the majority of fish spend less than 50 hr or 10 percent of the time at discharge temperatures. During 1974 a second fish tagging study was conducted, using temperature-sensitive tags to yield discharge residence times of Lake Michigan salmonids at Point Beach thermal discharge. Preliminary results revealed that many fish tag values were close to Unit I line indicating that calculated maximum discharge residence times for these fish will be nearly 100 percent of the elapsed time

  16. Passive wireless tags for tongue controlled assistive technology interfaces

    Science.gov (United States)

    Rakibet, Osman O.; Horne, Robert J.; Kelly, Stephen W.

    2016-01-01

    Tongue control with low profile, passive mouth tags is demonstrated as a human–device interface by communicating values of tongue-tag separation over a wireless link. Confusion matrices are provided to demonstrate user accuracy in targeting by tongue position. Accuracy is found to increase dramatically after short training sequences with errors falling close to 1% in magnitude with zero missed targets. The rate at which users are able to learn accurate targeting with high accuracy indicates that this is an intuitive device to operate. The significance of the work is that innovative very unobtrusive, wireless tags can be used to provide intuitive human–computer interfaces based on low cost and disposable mouth mounted technology. With the development of an appropriate reading system, control of assistive devices such as computer mice or wheelchairs could be possible for tetraplegics and others who retain fine motor control capability of their tongues. The tags contain no battery and are intended to fit directly on the hard palate, detecting tongue position in the mouth with no need for tongue piercings. PMID:27222736

  17. The bremsstrahlung tagged photon beam in Hall B at JLab

    Energy Technology Data Exchange (ETDEWEB)

    Sober, D.I.; Crannell, Hall; Longhi, Alberto; Matthews, S.K.; O' Brien, J.T. E-mail: obrienj@cua.edu; Berman, B.L.; Briscoe, W.J.; Cole, Philip L.; Connelly, J.P.; Dodge, W.R.; Murphy, L.Y.; Philips, S.A.; Dugger, M.K.; Lawrence, D.; Ritchie, B.G.; Smith, E.S.; Lambert, James M.; Anciant, E.; Audit, G.; Auger, T.; Marchand, C.; Klusman, M.; Napolitano, J.; Khandaker, M.A.; Salgado, C.W.; Sarty, A.J

    2000-02-01

    We describe the design and commissioning of the photon tagging beamline installed in experimental Hall B at the Thomas Jefferson National Accelerator Facility (JLab). This system can tag photon energies over a range from 20% to 95% of the incident electron energy, and is capable of operation with beam energies up to 6.1 GeV. A single dipole magnet is combined with a hodoscope containing two planar arrays of plastic scintillators to detect energy-degraded electrons from a thin bremsstrahlung radiator. The first layer of 384 partially overlapping small scintillators provides photon energy resolution, while the second layer of 61 larger scintillators provides the timing resolution necessary to form a coincidence with the corresponding nuclear interaction triggered by the tagged photon. The definitions of overlap channels in the first counter plane and of geometric correlation between the two planes are determined using digitized time information from the individual counters. Auxiliary beamline devices are briefly described, and performance results to date under real operating conditions are presented. The entire photon-tagging system has met or exceeded its design goals.

  18. Efficiency of Airborne Sample Analysis Platform (ASAP) bioaerosol sampler for pathogen detection

    OpenAIRE

    Sharma, Anurag; Clark, Elizabeth; McGlothlin, James D.; Mittal, Suresh K.

    2015-01-01

    The threat of bioterrorism and pandemics has highlighted the urgency for rapid and reliable bioaerosol detection in different environments. Safeguarding against such threats requires continuous sampling of the ambient air for pathogen detection. In this study we investigated the efficacy of the Airborne Sample Analysis Platform (ASAP) 2800 bioaerosol sampler to collect representative samples of air and identify specific viruses suspended as bioaerosols. To test this concept, we aerosolized an...

  19. Estimation for aerial detection effectiveness with cooperation efficiency factors of early-warning aircraft in early-warning detection SoS under BSC framework

    Science.gov (United States)

    Zhu, Feng; Hu, Xiaofeng; He, Xiaoyuan; Guo, Rui; Li, Kaiming; Yang, Lu

    2017-11-01

    In the military field, the performance evaluation of early-warning aircraft deployment or construction is always an important problem needing to be explored. As an effective approach of enterprise management and performance evaluation, Balanced Score Card (BSC) attracts more and more attentions and is studied more and more widely all over the world. It can also bring feasible ideas and technical approaches for studying the issue of the performance evaluation of the deployment or construction of early-warning aircraft which is the important component in early-warning detection system of systems (SoS). Therefore, the deep explored researches are carried out based on the previously research works. On the basis of the characteristics of space exploration and aerial detection effectiveness of early-warning detection SoS and the cardinal principle of BSC are analyzed simply, and the performance evaluation framework of the deployment or construction of early-warning aircraft is given, under this framework, aimed at the evaluation issue of aerial detection effectiveness of early-warning detection SoS with the cooperation efficiency factors of the early-warning aircraft and other land based radars, the evaluation indexes are further designed and the relative evaluation model is further established, especially the evaluation radar chart being also drawn to obtain the evaluation results from a direct sight angle. Finally, some practical computer simulations are launched to prove the validity and feasibility of the research thinking and technologic approaches which are proposed in the paper.

  20. An Efficient Algorithm for the Detection of Exposed and Hidden Wormhole Attack

    Directory of Open Access Journals (Sweden)

    ZUBAIR AHMED KHAN

    2016-07-01

    Full Text Available MANETs (Mobile Ad Hoc Networks are slowly integrating into our everyday lives, their most prominent uses are visible in the disaster and war struck areas where physical infrastructure is almost impossible or very hard to build. MANETs like other networks are facing the threat of malicious users and their activities. A number of attacks have been identified but the most severe of them is the wormhole attack which has the ability to succeed even in case of encrypted traffic and secure networks. Once wormhole is launched successfully, the severity increases by the fact that attackers can launch other attacks too. This paper presents a comprehensive algorithm for the detection of exposed as well as hidden wormhole attack while keeping the detection rate to maximum and at the same reducing false alarms. The algorithm does not require any extra hardware, time synchronization or any special type of nodes. The architecture consists of the combination of Routing Table, RTT (Round Trip Time and RSSI (Received Signal Strength Indicator for comprehensive detection of wormhole attack. The proposed technique is robust, light weight, has low resource requirements and provides real-time detection against the wormhole attack. Simulation results show that the algorithm is able to provide a higher detection rate, packet delivery ratio, negligible false alarms and is also better in terms of Ease of Implementation, Detection Accuracy/ Speed and processing overhead.

  1. Safety, tumor trafficking and immunogenicity of chimeric antigen receptor (CAR)-T cells specific for TAG-72 in colorectal cancer.

    Science.gov (United States)

    Hege, Kristen M; Bergsland, Emily K; Fisher, George A; Nemunaitis, John J; Warren, Robert S; McArthur, James G; Lin, Andy A; Schlom, Jeffrey; June, Carl H; Sherwin, Stephen A

    2017-01-01

    T cells engineered to express chimeric antigen receptors (CARs) have established efficacy in the treatment of B-cell malignancies, but their relevance in solid tumors remains undefined. Here we report results of the first human trials of CAR-T cells in the treatment of solid tumors performed in the 1990s. Patients with metastatic colorectal cancer (CRC) were treated in two phase 1 trials with first-generation retroviral transduced CAR-T cells targeting tumor-associated glycoprotein (TAG)-72 and including a CD3-zeta intracellular signaling domain (CART72 cells). In trial C-9701 and C-9702, CART72 cells were administered in escalating doses up to 10 10 total cells; in trial C-9701 CART72 cells were administered by intravenous infusion. In trial C-9702, CART72 cells were administered via direct hepatic artery infusion in patients with colorectal liver metastases. In both trials, a brief course of interferon-alpha (IFN-α) was given with each CART72 infusion to upregulate expression of TAG-72. Fourteen patients were enrolled in C-9701 and nine in C-9702. CART72 manufacturing success rate was 100% with an average transduction efficiency of 38%. Ten patients were treated in CC-9701 and 6 in CC-9702. Symptoms consistent with low-grade, cytokine release syndrome were observed in both trials without clear evidence of on target/off tumor toxicity. Detectable, but mostly short-term (≤14 weeks), persistence of CART72 cells was observed in blood; one patient had CART72 cells detectable at 48 weeks. Trafficking to tumor tissues was confirmed in a tumor biopsy from one of three patients. A subset of patients had 111 Indium-labeled CART72 cells injected, and trafficking could be detected to liver, but T cells appeared largely excluded from large metastatic deposits. Tumor biomarkers carcinoembryonic antigen (CEA) and TAG-72 were measured in serum; there was a precipitous decline of TAG-72, but not CEA, in some patients due to induction of an interfering antibody to the TAG-72

  2. Efficient capture, rapid killing and ultrasensitive detection of bacteria by a nano-decorated multi-functional electrode sensor.

    Science.gov (United States)

    Wu, Runrun; Ma, Yue; Pan, Jianming; Lee, Shih-Hui; Liu, Jinxin; Zhu, Hengjia; Gu, Runxin; Shea, Kenneth J; Pan, Guoqing

    2018-03-15

    In this work, we demonstrated a nano-decorated porous impedance electrode sensor for efficient capture, rapid killing and ultrasensitive detection of bacteria. The multi-functional sensor was prepared by a facile sonochemical method via in situ deposition of antibacterial prickly Zn-CuO nanoparticles and graphene oxide (GO) nanosheets on a Ni porous electrode. Due to the surface burr-like nanostructures, the nano-decorated impedance sensor exhibited very good bacterial-capture efficiency (70 - 80% in 20min) even at a low concentration of 50 CFU mL -1 , rapid antibacterial rate (100% killing in 30min) and high detection sensitivity (as low as 10 CFU mL -1 ). More importantly, the nano-decorated sensor has proven to be highly effective in quantitative detection of bacteria in a biological sample, for example, a rat blood sample spiked with E. coli. Despite the complexity of blood, the sensor still exhibited excellent detection precision within 30min at bacteria concentrations ranging from 10 - 10 5 CFU mL -1 . The simplicity, rapidity, sensitivity, practicability and multifunctionality of this impedance sensor would greatly facilitate applications in portable medical devices for on-the-spot diagnosis and even the possibility for simultaneous therapy of diseases caused by bacterial infections. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Measuring abnormal movements in free-swimming fish with accelerometers: implications for quantifying tag and parasite load.

    Science.gov (United States)

    Broell, Franziska; Burnell, Celene; Taggart, Christopher T

    2016-03-01

    Animal-borne data loggers allow movement, associated behaviours and energy expenditure in fish to be quantified without direct observations. As with any tagging, tags that are attached externally may adversely affect fish behaviour, swimming efficiency and survival. We report on free-swimming wild Atlantic cod (Gadus morhua) held in a large mesocosm that exhibited distinctly aberrant rotational swimming (scouring) when externally tagged with accelerometer data loggers. To quantify the phenomenon, the cod were tagged with two sizes of loggers (18 and 6 g; parasite-load shedding in the wild. The results infer a more careful interpretation of data derived from external tags and the careful consideration of tag type, drag, buoyancy and placement, as well as animal buoyancy and species. © 2016. Published by The Company of Biologists Ltd.

  4. Calculation of efficiency of high-energy neutron detection by plastic scintillators

    International Nuclear Information System (INIS)

    Telegin, Yu.N.

    1977-01-01

    A computer was used to calculate neutron (5-30O MeV) registration effeciencies with plastic scintillators 2,5,10, 20,30,40 and 50 cm thick. The results are shown in the form of tables. The contributions to efficiency of various processes have been analysed. The calculation results may be used in planning experiments with neutron counters

  5. Method for detecting binding efficiencies of synthetic oligonucleotides: Targeting bacteria and insects

    Science.gov (United States)

    Expanding applications of gene-based targeting biotechnology in functional genomics and the treatment of plants, animals, and microbes has synergized the need for new methods to measure binding efficiencies of these products to their genetic targets. The adaptation and innovative use of Cell–Penetra...

  6. Efficiency of a Strategy for Detecting Back Random Responding on the Personality Assessment Inventory

    Science.gov (United States)

    Morey, Leslie C.; Hopwood, Christopher J.

    2004-01-01

    The efficiency of a new strategy to identify back random responding (BRR) on the Personality Assessment Inventory (L. C. Morey, 1991) was studied using random manipulations of item responses from community (904 adult nonpatients) and clinical (1,079 adult patients) respondents. The strategy involved a comparison of short-form and full-instrument T…

  7. ROXAS – an efficient and accurate tool to detect vessels in diffuse-porous species

    NARCIS (Netherlands)

    Wegner, L.; Arx, von G.; Sass, U.G.W.; Eilmann, B.

    2013-01-01

    Wood-anatomical parameters form a valuable archive to study past limitations on tree growth and act as a link between dendrochronology and ecophysiology. Yet, analysing these parameters is a time-consuming procedure and only few long chronologies exist. To increase measurement efficiency of

  8. Use of impedance tagging to monitor fuel cell stack performance

    Science.gov (United States)

    Silva, Gregory

    Fuel cells are electrochemical device that are traditionally assembled in stacks to perform meaningful work. Monitoring the state of the stack is vitally important to ensure that it is operating efficiently and that constituent cells are not failing for one of a several common reasons including membrane dehydration, gas diffusion layer flooding, reactant starvation, and physical damage. Current state-of-the-art monitoring systems are costly and require at least one connection per cell on the stack, which introduces reliability concerns for stacks consisting of hundreds of cells. This thesis presents a novel approach for diagnosing problems in a fuel cell stack that attempts to reduce the cost and complexity of monitoring cells in a stack. The proposed solution modifies the electrochemical impedance spectroscopy (EIS) response of each cell in the stack by connecting an electrical tag in parallel with each cell. This approach allows the EIS response of the entire stack to identify and locate problems in the stack. Capacitors were chosen as tags because they do not interfere with normal stack operation and because they can generate distinct stack EIS responses. An experiment was performed in the Center for Automation Technologies an Systems (CATS) fuel cell laboratory at Rensselaer Polytechnic Institute (RPI) to perform EIS measurements on a single cell with and without capacitor tags to investigate the proposed solution. The EIS data collected from this experiment was used to create a fuel cell model to investigate the proposed solution under ideal conditions. This thesis found that, although the concept shows some promise in simulations, significant obstacles to implementing the proposed solution. Observed EIS response when the capacitor tags were connected did not match the expected EIS response. Constraints on the capacitor tags found by the model impose significant manufacturing challenges to the proposed solution. Further development of the proposed solution is

  9. Efficient Forest Fire Detection Index for Application in Unmanned Aerial Systems (UASs

    Directory of Open Access Journals (Sweden)

    Henry Cruz

    2016-06-01

    Full Text Available This article proposes a novel method for detecting forest fires, through the use of a new color index, called the Forest Fire Detection Index (FFDI, developed by the authors. The index is based on methods for vegetation classification and has been adapted to detect the tonalities of flames and smoke; the latter could be included adaptively into the Regions of Interest (RoIs with the help of a variable factor. Multiple tests have been performed upon database imagery and present promising results: a detection precision of 96.82% has been achieved for image sizes of 960 × 540 pixels at a processing time of 0.0447 seconds. This achievement would lead to a performance of 22 f/s, for smaller images, while up to 54 f/s could be reached by maintaining a similar detection precision. Additional tests have been performed on fires in their early stages, achieving a precision rate of p = 96.62%. The method could be used in real-time in Unmanned Aerial Systems (UASs, with the aim of monitoring a wider area than through fixed surveillance systems. Thus, it would result in more cost-effective outcomes than conventional systems implemented in helicopters or satellites. UASs could also reach inaccessible locations without jeopardizing people’s safety. On-going work includes implementation into a commercially available drone.

  10. SGR: A New Efficient Kernel for Outlier Detection in Sensor Data Minimizing Mise

    Directory of Open Access Journals (Sweden)

    Seema SHARMA

    2015-06-01

    Full Text Available In sensor network, collected data is error prone due to errors during sensors and transmission. Sometimes, the sensed data may appear to be erroneous due to large deviation from normal data distribution. Such data points termed as outliers may contain some important pattern. Outliers, if neglected as erroneous data, may result in failure to detect important phenomenon. Hence, it is necessary to not only detect such data points but analyze them further to establish the reason behind such data values. The presence of outliers may distort contained information. To ensure that the information is correctly extracted, it is necessary to identify the outliers and isolate them during knowledge extraction phase. In this paper, we propose a novel unsupervised algorithm for detecting outliers based on density by coupling two principles: first, kernel density estimation and second assigning an outlier score to each object. A new kernel function building a smoother version of density estimate is proposed. An outlier score is assigned to each object by comparing local density estimate of each object to its neighbors. The two steps provide a framework for outlier detection that can be easily applied to discover new or unusual types of outliers. Experiments performed on synthetic and real datasets suggest that the proposed approach can detect outliers precisely and achieve high recall rates which in turn demonstrate the potency of the proposed approach.

  11. Improved efficiency access control equipment and explosive, weapons and drug abuse detection

    Energy Technology Data Exchange (ETDEWEB)

    Jenkins, A.; Milford, A.; Woollven, J.

    1985-01-01

    The second generation portal explosives detector has been designed with increased detection capability and convenience in service. The method of detection and performance relative to the first generation is described. A novel method of auto-calibration and self diagnosis is described and results are discussed. Improvements in convenience of operation have been achieved and operating space and costs reduced by combining metal detection capability, together with explosives detection. This allows both alarm signal and diagnostic outputs to be combined on a single remote panel in the guard room, and reduces the number of guards needed to man the access control. This type of access control is entirely a defensive measure against attack but a further additional feature is proposed which will also check the state of mind of all personnel passing through the check point. Any person suffering from the effect of narcotic or alcohol will be detected by their inability to reproduce their normal signature. A new method of signature analysis in five dimensions is described together with proposals for integrating the check without increasing the time in the test area. Some recent results on the effects of alcohol on signature reproduction is given.

  12. Engineering the ATLAS TAG Browser

    International Nuclear Information System (INIS)

    Zhang Qizhi

    2011-01-01

    ELSSI is a web-based event metadata (TAG) browser and event-level selection service for ATLAS. In this paper, we describe some of the challenges encountered in the process of developing ELSSI, and the software engineering strategies adopted to address those challenges. Approaches to management of access to data, browsing, data rendering, query building, query validation, execution, connection management, and communication with auxiliary services are discussed. We also describe strategies for dealing with data that may vary over time, such as run-dependent trigger decision decoding. Along with examples, we illustrate how programming techniques in multiple languages (PHP, JAVASCRIPT, XML, AJAX, and PL/SQL) have been blended to achieve the required results. Finally, we evaluate features of the ELSSI service in terms of functionality, scalability, and performance.

  13. A new tagging system for production of recombinant proteins in Drosophila S2 cells using the third domain of the urokinase receptor

    DEFF Research Database (Denmark)

    Gårdsvoll, Henrik; Hansen, Line V; Jørgensen, Thomas J D

    2007-01-01

    The use of protein fusion tag technology greatly facilitates detection, expression and purification of recombinant proteins, and the demands for new and more effective systems are therefore expanding. We have used a soluble truncated form of the third domain of the urokinase receptor...... as a convenient C-terminal fusion partner for various recombinant extracellular human proteins used in basic cancer research. The stability of this cystein-rich domain, which structure adopts a three-finger fold, provides an important asset for its applicability as a fusion tag for expression of recombinant...... proteins. Up to 20mg of intact fusion protein were expressed by stably transfected Drosophila S2 cells per liter of culture using this strategy. Purification of these secreted fusion proteins from the conditioned serum free medium of S2 cells was accompanied by an efficient one-step immunoaffinity...

  14. Efficient extraction and detection of aromatic toxicants from crude oil and tar balls using multiple cyclodextrin derivatives.

    Science.gov (United States)

    Serio, Nicole; Levine, Mindy

    2015-06-15

    Herein we report the efficient extraction of aromatic analytes from crude oil and tar balls using multiple cyclodextrin derivatives. The known propensity of the cyclodextrins to bind hydrophobic guests in their hydrophobic interiors enhanced the extraction of aromatic analytes from the oil layer to the aqueous layer, with methyl-β-cyclodextrin and β-cyclodextrin providing the most significant enhancement in extraction efficiencies of aromatic toxicants (69% aromatic toxicants in aqueous layer in the presence of methyl-β-cyclodextrin compared to 47% in cyclodextrin-free solution for tar ball oil extraction), and provide optimal tunability for developing efficient extraction systems. The cyclodextrin derivatives also promoted efficient energy transfer in the aqueous solutions, with up to 86% efficient energy transfer observed in the presence of γ-cyclodextrin compared to 50% in the absence of cyclodextrin for oil spill oil extraction. Together, this dual function extraction followed by detection system has potential in the development of environmental remediation systems. Copyright © 2015 Elsevier Ltd. All rights reserved.

  15. To tag or not to tag: animal welfare, conservation and stakeholder considerations in fish tracking studies that use electronic tags

    Energy Technology Data Exchange (ETDEWEB)

    Cooke, Steven J.; Nguyen, Vivian M.; Murchie, Karen J.; Thiem, Jason D.; Donaldson, Michael R.; Hinch, Scott G.; Brown, Richard S.; Fisk, Aaron

    2013-11-01

    The advent and widespread adoption of electronic tags (including biotelemetry and biologging devices) for tracking animals has provided unprecedented information on the biology, management, and conservation of fish in the world’s oceans and inland waters. However, use of these tools is not without controversy. Even when scientific and management objectives may best be achieved using electronic tags, it is increasingly important to further consider other factors such as the welfare of tagged animals (i.e., the role of training and science-based surgical guidelines, anesthetic use, inability to maintain sterile conditions in field environments), the ethics of tagging threatened species vs. using surrogates, stakeholder perspectives on tagging (including aboriginals), as well as use of data emanating from such studies (e.g., by fishers to facilitate exploitation). Failure to do so will have the potential to create conflict and undermine scientific, management and public confidence in the use of this powerful tool. Indeed, there are already a number of examples of where tracking studies using electronic tags have been halted based on concerns raised by researchers, authorities, or stakeholders. Here we present a candid evaluation of several factors that should be considered when determining when to tag or not to tag fish with electronic devices. It is not our objective to judge the merit of previous studies. Rather, we hope to stimulate debate and discussion regarding the use of electronic tags to study fish. Relatedly, there is a need for more research to address these questions (e.g., what level of cleanliness is needed when conducting surgeries, what type of training should be required for fish surgery) including human dimensions studies to understand perspectives of different actors including society as a whole with respect to tagging and tracking studies.

  16. StereoBox: A Robust and Efficient Solution for Automotive Short-Range Obstacle Detection

    Directory of Open Access Journals (Sweden)

    Alberto Broggi

    2007-07-01

    Full Text Available This paper presents a robust method for close-range obstacle detection with arbitrarily aligned stereo cameras. System calibration is performed by means of a dense grid to remove perspective and lens distortion after a direct mapping between image pixels and world points. Obstacle detection is based on the differences between left and right images after transformation phase and with a polar histogram, it is possible to detect vertical structures and to reject noise and small objects. Found objects' world coordinates are transmitted via CAN bus; the driver can also be warned through an audio interface. The proposed algorithm can be useful in different automotive applications, requiring real-time segmentation without any assumption on background. Experimental results proved the system to be robust in several envitonmental conditions. In particular, the system has been tested to investigate presence of obstacles in blind spot areas around heavy goods vehicles (HGVs and has been mounted on three different prototypes at different heights.

  17. StereoBox: A Robust and Efficient Solution for Automotive Short-Range Obstacle Detection

    Directory of Open Access Journals (Sweden)

    Broggi Alberto

    2007-01-01

    Full Text Available This paper presents a robust method for close-range obstacle detection with arbitrarily aligned stereo cameras. System calibration is performed by means of a dense grid to remove perspective and lens distortion after a direct mapping between image pixels and world points. Obstacle detection is based on the differences between left and right images after transformation phase and with a polar histogram, it is possible to detect vertical structures and to reject noise and small objects. Found objects' world coordinates are transmitted via CAN bus; the driver can also be warned through an audio interface. The proposed algorithm can be useful in different automotive applications, requiring real-time segmentation without any assumption on background. Experimental results proved the system to be robust in several envitonmental conditions. In particular, the system has been tested to investigate presence of obstacles in blind spot areas around heavy goods vehicles (HGVs and has been mounted on three different prototypes at different heights.

  18. Efficient Proximity Detection among Mobile Users via SelfTuning Policies

    DEFF Research Database (Denmark)

    Yiu, Man Lung; Hou U, Leong; Saltenis, Simonas

    2010-01-01

    applications and massively multiplayer online games. Existing proximity detection solutions either incur substantial location update costs or their performance does not scale well to a large number of users. Motivated by this, we present a centralized proximity detection solution that assigns each mobile......Given a set of users, their friend relationships, and a distance threshold per friend pair, the proximity detection problem is to find each pair of friends such that the Euclidean distance between them is within the given threshold. This problem plays an essential role in friend-locator...... client with a mobile region. We then design a self-tuning policy to adjust the radius of the region automatically, in order to minimize communication cost. In addition, we analyze the communication cost of our solutions, and provide valuable insights on their behaviors. Extensive experiments suggest...

  19. Efficient examination to detect the location of cancer in cases with positive sputum cytology

    International Nuclear Information System (INIS)

    Moriya, Hiroshi; Yaginuma, Koji; Shibuya, Hiroko

    1993-01-01

    In order to localize cancers in 55 cases with positive sputum cytology, we examined chest CT and otolaryngeal findings, in addition to performing bronchoscopic examinations. Consequently, 30 cases had lung cancer, 5 had laryngopharyngeal cancer and 3 had cancer of the oral cavity. Otolaryngeal observation was useful for detection of these cancers of the upper respiratory tract. In 30 lung cancers, 23 were roentgenographically occult cancers. But, among these 23, 12 had positive findings on chest CT. CT was useful for the cases of roentgenographically occult lung cancer. Especially, CT was very effective for detection of small cancer lesions in the peripheral lung, which were undetectable bronchofiberscopically. Three of 20 cases, in which no cancers were detected after the initial examination, had cancer lesions 1-2 years later. These results suggest that CT, otolaryngeal observation and intensive follow-up of undetectable cases are useful for localizing cancer in cases with positive sputum cytology. (author)

  20. Efficient Error Detection in Soft Data Fusion for Cooperative Spectrum Sensing

    KAUST Repository

    Saqib Bhatti, Dost Muhammad

    2018-03-18

    The primary objective of cooperative spectrum sensing (CSS) is to determine whether a particular spectrum is occupied by a licensed user or not, so that unlicensed users called secondary users (SUs) can utilize that spectrum, if it is not occupied. For CSS, all SUs report their sensing information through reporting channel to the central base station called fusion center (FC). During transmission, some of the SUs are subjected to fading and shadowing, due to which the overall performance of CSS is degraded. We have proposed an algorithm which uses error detection technique on sensing measurement of all SUs. Each SU is required to re-transmit the sensing data to the FC, if error is detected on it. Our proposed algorithm combines the sensing measurement of limited number of SUs. Using Proposed algorithm, we have achieved the improved probability of detection (PD) and throughput. The simulation results compare the proposed algorithm with conventional scheme.

  1. Comparing different types of patagial tags for use on vultures ...

    African Journals Online (AJOL)

    Raptor research often requires identifying individuals. Researchers place patagial tags on raptors to facilitate such identification. Researchers in southern African use two main types of patagial tags: hard plastic ear tags originally designed for cattle and soft vinyl tags. We deployed both types of tags on vultures in Botswana.

  2. Comparing different types of patagial tags for use on vultures ...

    African Journals Online (AJOL)

    Researchers in southern African use two main types of patagial tags: hard plastic ear tags originally designed for cattle and soft vinyl tags. We deployed both types of tags on vultures in Botswana. Based on our observations, we recommend using soft vinyl tags as they appear to be more aerodynamic and can be read from ...

  3. Efficiency of Individual Tree Detection Approaches Based on Light-Weight and Low-Cost UAS Imagery in Australian Savannas

    Directory of Open Access Journals (Sweden)

    Grigorijs Goldbergs

    2018-01-01

    Full Text Available The reliability of airborne light detection and ranging (LiDAR for delineating individual trees and estimating aboveground biomass (AGB has been proven in a diverse range of ecosystems, but can be difficult and costly to commission. Point clouds derived from structure from motion (SfM matching techniques obtained from unmanned aerial systems (UAS could be a feasible low-cost alternative to airborne LiDAR scanning for canopy parameter retrieval. This study assesses the extent to which SfM three-dimensional (3D point clouds—obtained from a light-weight mini-UAS quadcopter with an inexpensive consumer action GoPro camera—can efficiently and effectively detect individual trees, measure tree heights, and provide AGB estimates in Australian tropical savannas. Two well-established canopy maxima and watershed segmentation tree detection algorithms were tested on canopy height models (CHM derived from SfM imagery. The influence of CHM spatial resolution on tree detection accuracy was analysed, and the results were validated against existing high-resolution airborne LiDAR data. We found that the canopy maxima and watershed segmentation routines produced similar tree detection rates (~70% for dominant and co-dominant trees, but yielded low detection rates (<35% for suppressed and small trees due to poor representativeness in point clouds and overstory occlusion. Although airborne LiDAR provides higher tree detection rates and more accurate estimates of tree heights, we found SfM image matching to be an adequate low-cost alternative for the detection of dominant and co-dominant tree stands.

  4. Evaluation of PIT-tagging in cyprinids

    DEFF Research Database (Denmark)

    Skov, Christian; Brodersen, J.; Brönmark, C.

    2005-01-01

    Laboratory and field experiments were used to investigate how different marking procedures, with 23 mm PIT (passive integrated transponders) - tags. affected mortality, body condition and tag expulsion in small roach Rutilus rutilus and rudd Scardinus erythrophthalmus (117 to 163 mm total length...

  5. Deep Learning in Flavour Tagging at the ATLAS experiment

    CERN Document Server

    Lanfermann, Marie Christine; The ATLAS collaboration

    2017-01-01

    A novel higher-level flavour tagging algorithm called DL1 has been developed using a neural network at the ATLAS experiment at the CERN Large Hadron Collider. We have investigated the potential of Deep Learning in flavour tagging using higher-level inputs from lower-level physics-motivated taggers. A systematic grid search over architectures and the training hyperparameter space is presented. In this novel neural network approach, the jet flavours are treated on an equal footing while training with multiple output nodes, which provides a highly flexible tagger. The DL1 studies presented show that the obtained neural network improves discrimination against both light-jets and c-jets, and also provides a novel c-tagging possibility. The performance for arbitrary background mixtures can be fine-tuned after the training by using iso-efficiency lines of constant signal efficiency, according to the to the needs of the physics analysis. The resulting DL1 tagger is described and a detailed set of performance plots pr...

  6. Efficient voice activity detection in reverberant enclosures using far field microphones

    DEFF Research Database (Denmark)

    Petsatodis, Theodore; Boukis, Christos

    2009-01-01

    An algorithm suitable for voice activity detection under reverberant conditions is proposed in this paper. Due to the use of far-filed microphones the proposed solution processes speech signals of highly-varying intensity and signal to noise ratio, that are contaminated with several echoes...

  7. Convolution Comparison Pattern: An Efficient Local Image Descriptor for Fingerprint Liveness Detection.

    Science.gov (United States)

    Gottschlich, Carsten

    2016-01-01

    We present a new type of local image descriptor which yields binary patterns from small image patches. For the application to fingerprint liveness detection, we achieve rotation invariant image patches by taking the fingerprint segmentation and orientation field into account. We compute the discrete cosine transform (DCT) for these rotation invariant patches and attain binary patterns by comparing pairs of two DCT coefficients. These patterns are summarized into one or more histograms per image. Each histogram comprises the relative frequencies of pattern occurrences. Multiple histograms are concatenated and the resulting feature vector is used for image classification. We name this novel type of descriptor convolution comparison pattern (CCP). Experimental results show the usefulness of the proposed CCP descriptor for fingerprint liveness detection. CCP outperforms other local image descriptors such as LBP, LPQ and WLD on the LivDet 2013 benchmark. The CCP descriptor is a general type of local image descriptor which we expect to prove useful in areas beyond fingerprint liveness detection such as biological and medical image processing, texture recognition, face recognition and iris recognition, liveness detection for face and iris images, and machine vision for surface inspection and material classification.

  8. Designing efficient surveys: spatial arrangement of sample points for detection of invasive species

    Czech Academy of Sciences Publication Activity Database

    Berec, Luděk; Kean, J. M.; Epanchin-Niell, R.; Liebhold, A. M.; Haight, R. G.

    2015-01-01

    Roč. 17, č. 1 (2015), s. 445-459 ISSN 1387-3547 Grant - others:National Science Foundation(US) DEB-0553768 Institutional support: RVO:60077344 Keywords : biosecurity * early pest detection * eradication Subject RIV: EH - Ecology, Behaviour Impact factor: 2.855, year: 2015 http://link.springer.com/article/10.1007%2Fs10530-014-0742-x

  9. Efficient snoring and breathing detection based on sub-band spectral statistics.

    Science.gov (United States)

    Sun, Xiang; Kim, Jin Young; Won, Yonggwan; Kim, Jung-Ja; Kim, Kyung-Ah

    2015-01-01

    Snoring, a common symptom in the general population may indicate the presence of obstructive sleep apnea (OSA). In order to detect snoring events in sleep sound recordings, a novel method was proposed in this paper. The proposed method operates by analyzing the acoustic characteristics of the snoring sounds. Based on these acoustic properties, the feature vectors are obtained using the mean and standard deviation of the sub-band spectral energy. A support vector machine is then applied to perform the frame-based classification procedure. This method was demonstrated experimentally to be effective for snoring detection. The database for detection included full-night audio recordings from four individuals who acknowledged having snoring habits. The performance of the proposed method was evaluated by classifying different events (snoring, breathing and silence) from the sleep sound recordings and comparing the classification against ground truth. The proposed algorithm was able to achieve an accuracy of 99.61% for detecting snoring events, 99.16% for breathing, and 99.55% for silence.

  10. Fast and efficient detection of tuberculosis antigens using liposome encapsulated secretory proteins of Mycobacterium tuberculosis

    Directory of Open Access Journals (Sweden)

    Dileep Tiwari

    2017-04-01

    Conclusion: Our study demonstrated that the newly developed liposome tuberculosis antigen card test detected antigens in our study population with approximately 97.48% sensitivity and 95.79% specificity. This is the first study to report the liposomal encapsulation of culture filtrate proteins from M. tuberculosis for diagnostic application.

  11. Efficient strategy for detecting gene × gene joint action and its application in schizophrenia

    NARCIS (Netherlands)

    Won, Sungho; Kwon, Min-Seok; Mattheisen, Manuel; Park, Suyeon; Park, Changsoon; Kihara, Daisuke; Cichon, Sven; Ophoff, Roel; Nöthen, Markus M.; Rietschel, Marcella; Baur, Max; Uitterlinden, Andre G.; Hofmann, A.; Lange, Christoph; Kahn, René S.; Linszen, Don H.; van Os, Jim; Wiersma, Durk; Bruggeman, Richard; Cahn, Wiepke; de Haan, Lieuwe; Krabbendam, Lydia; Myin-Germeys, Inez

    2014-01-01

    We propose a new approach to detect gene × gene joint action in genome-wide association studies (GWASs) for case-control designs. This approach offers an exhaustive search for all two-way joint action (including, as a special case, single gene action) that is computationally feasible at the

  12. A synthesis of tagging studies examining the behaviour and survival of anadromous salmonids in marine environments.

    Directory of Open Access Journals (Sweden)

    S Matthew Drenner

    Full Text Available This paper synthesizes tagging studies to highlight the current state of knowledge concerning the behaviour and survival of anadromous salmonids in the marine environment. Scientific literature was reviewed to quantify the number and type of studies that have investigated behaviour and survival of anadromous forms of Pacific salmon (Oncorhynchus spp., Atlantic salmon (Salmo salar, brown trout (Salmo trutta, steelhead (Oncorhynchus mykiss, and cutthroat trout (Oncorhynchus clarkii. We examined three categories of tags including electronic (e.g. acoustic, radio, archival, passive (e.g. external marks, Carlin, coded wire, passive integrated transponder [PIT], and biological (e.g. otolith, genetic, scale, parasites. Based on 207 papers, survival rates and behaviour in marine environments were found to be extremely variable spatially and temporally, with some of the most influential factors being temperature, population, physiological state, and fish size. Salmonids at all life stages were consistently found to swim at an average speed of approximately one body length per second, which likely corresponds with the speed at which transport costs are minimal. We found that there is relatively little research conducted on open-ocean migrating salmonids, and some species (e.g. masu [O. masou] and amago [O. rhodurus] are underrepresented in the literature. The most common forms of tagging used across life stages were various forms of external tags, coded wire tags, and acoustic tags, however, the majority of studies did not measure tagging/handling effects on the fish, tag loss/failure, or tag detection probabilities when estimating survival. Through the interdisciplinary application of existing and novel technologies, future research examining the behaviour and survival of anadromous salmonids could incorporate important drivers such as oceanography, tagging/handling effects, predation, and physiology.

  13. Novel Use of PIT Tags in Sea Cucumbers: Promising Results with the Commercial Species Cucumaria frondosa.

    Directory of Open Access Journals (Sweden)

    Bruno L Gianasi

    Full Text Available The lack of a reliable and innocuous mark-recapture method has limited studies that would provide essential information for the management of commercial sea cucumbers. Tagging sea cucumbers is notoriously difficult because of their plastic nature and autolysis capacities. The markers that have so far been tested, mainly on or through the body wall, were either lost rapidly or had major drawbacks (e.g. suitable only for batch identification, requiring complex analysis, causing infections, necrosis, behavioural changes and mortality. The present study explored the efficacy of passive integrated transponder (PIT tags for individually marking sea cucumbers by assessing retention rates and long-term side effects of tags inserted in previously unstudied tissues/organs. Individuals of the species Cucumaria frondosa were tagged in the body wall, aquapharyngeal bulb and at the base of the oral tentacles. They were monitored closely for evidence of stress, infection, change in feeding and spawning behaviour and tag retention rate. Implanting the tag in an oral tentacle to reach the hydrovascular system of the aquapharyngeal bulb achieved the best retention rates in full-size individuals: from a maximum of 92% after 30 days to 68% at the end of the experimental period (300 days. Efficacy was lower in smaller individuals (84% after 30 d and 42% after 300 d. Following a slight increase in cloacal movements for 15 h post tagging, no side effect was noted in sea cucumbers tagged in the aquapharyngeal bulb via the tentacles. Feeding and spawning behaviours were not affected and no signs of infections or abnormal cell development in the vicinity of the tags were observed. This study indicates that marking sea cucumbers with 8.2 mm long PIT tags implanted via the oral tentacle is an effective technique, yielding relatively high retention rates over long periods without any detectable physiological or behavioural effects.

  14. Development of an economic and efficient strategy to detect HBsAg: application of "gray-zones" in ELISA and combined use of several detection assays.

    Science.gov (United States)

    Peng, Jing; Cheng, Liming; Yin, Botao; Guan, Qing; Liu, Yanling; Wu, Shiji; Wang, Bin; Tang, Ning; Zhang, Biyu; Wang, Lian; Yang, Dongliang; Sun, Ziyong

    2011-11-20

    ELISA and CMIA are commonly used for detection of HBsAg. However, few investigations have been performed to evaluate their value in clinical practice, especially when jointly used. A reasonable and economic HBsAg testing algorithm is in great need. A total of 161,426 specimens in China were tested for 5 serum HBV markers with commonly used ELISA kits. 498 of these specimens were further tested for HBsAg by another ELISA kit, a CMIA kit and an HBsAg confirmatory assay. The sensitivities of the 2 ELISA kits were 76.21% and 88.42%, respectively. However, when using "gray-zones", the sensitivities were significantly improved to 97.43% and 96.43%. Furthermore, the combined use of the 2 ELISA kits and their "gray-zones" improved the sensitivity to 99.04%. Nevertheless, 2.91% of the samples with S/CO values below the lower "gray-zone" limits were reactive by the CMIA kit and then confirmed as HBsAg positive. However, 71.43% of the samples with HBsAg values within 0.05 and 0.10 IU/ml detected by the CMIA kit could not be confirmed. As a rational and economic strategy, combined use of "gray-zones" in ELISA and several different detection assays can significantly increase the efficiency of HBsAg detection. Copyright © 2011 Elsevier B.V. All rights reserved.

  15. Sentiment topic mining based on comment tags

    Science.gov (United States)

    Zhang, Daohai; Liu, Xue; Li, Juan; Fan, Mingyue

    2018-03-01

    With the development of e-commerce, various comments based on tags are generated, how to extract valuable information from these comment tags has become an important content of business management decisions. This study takes HUAWEI mobile phone tags as an example using the sentiment analysis and topic LDA mining method. The first step is data preprocessing and classification of comment tag topic mining. And then make the sentiment classification for comment tags. Finally, mine the comments again and analyze the emotional theme distribution under different sentiment classification. The results show that HUAWEI mobile phone has a good user experience in terms of fluency, cost performance, appearance, etc. Meanwhile, it should pay more attention to independent research and development, product design and development. In addition, battery and speed performance should be enhanced.

  16. Measurement of Fission Neutron Spectrum and Multiplicity using a Gamma Tag Double Time-of-flight Setup

    Science.gov (United States)

    Blain, E.; Daskalakis, A.; Danon, Y.

    2014-05-01

    Recent efforts have been made to improve the prompt fission neutron spectrum and nu-bar measurements for Uranium and Plutonium isotopes particularly in the keV region. A system has been designed at Rensselaer Polytechnic Institute (RPI) utilizing an array of EJ-301 liquid scintillators as well as lithium glass and plastic scintillators to experimentally determine these values. An array of BaF2 detectors was recently obtained from Oak Ridge National Laboratory to be used in conjunction with the neutron detectors. The system uses a novel gamma tagging method for fission which can offer an improvement over conventional fission chambers due to increased sample mass. A coincidence requirement on the gamma detectors from prompt fission gammas is used as the fission tag for the system as opposed to fission fragments in a conventional fission chamber. The system utilizes pulse digitization using Acqiris 8 bit digitizer boards which allow for gamma/neutron pulse height discrimination on the liquid scintillators during post processing. Additionally, a 252Cf fission chamber was designed and constructed at RPI which allowed for optimization and testing of the system without the need for an external neutron source. The characteristics of the gamma tagging method such as false detection rate and detection efficiency were determined using this fission chamber and verified using MCNP Polimi modeling. Prompt fission neutron spectrum data has been taken using the fission chamber focusing on the minimum detectable neutron energy for each of the various detectors. Plastic scintillators were found to offer a significant improvement over traditional liquid scintillators allowing energy measurements down to 50 keV. Background was also characterized for all detectors and will be discussed.

  17. More efficient integrated safeguards by applying a reasonable detection probability for maintaining low presence probability of undetected nuclear proliferating activities

    International Nuclear Information System (INIS)

    Otsuka, Naoto

    2013-01-01

    Highlights: • A theoretical foundation is presented for more efficient Integrated Safeguards (IS). • Probability of undetected nuclear proliferation activities should be maintained low. • For nations under IS, the probability to start proliferation activities is very low. • The fact can decrease the detection probability of IS by dozens of percentage points. • The cost of IS per nation can be cut down by reducing inspection frequencies etc. - Abstract: A theoretical foundation is presented for implementing more efficiently the present International Atomic Energy Agency (IAEA) integrated safeguards (ISs) on the basis of fuzzy evaluation of the probability that the evaluated nation will continue peaceful activities. It is shown that by determining the presence probability of undetected nuclear proliferating activities, nations under IS can be maintained at acceptably low proliferation risk levels even if the detection probability of current IS is decreased by dozens of percentage from the present value. This makes it possible to reduce inspection frequency and the number of collected samples, allowing the IAEA to cut costs per nation. This will contribute to further promotion and application of IS to more nations by the IAEA, and more efficient utilization of IAEA resources from the viewpoint of whole IS framework

  18. An Optimal Bahadur-Efficient Method in Detection of Sparse Signals with Applications to Pathway Analysis in Sequencing Association Studies.

    Directory of Open Access Journals (Sweden)

    Hongying Dai

    Full Text Available Next-generation sequencing data pose a severe curse of dimensionality, complicating traditional "single marker-single trait" analysis. We propose a two-stage combined p-value method for pathway analysis. The first stage is at the gene level, where we integrate effects within a gene using the Sequence Kernel Association Test (SKAT. The second stage is at the pathway level, where we perform a correlated Lancaster procedure to detect joint effects from multiple genes within a pathway. We show that the Lancaster procedure is optimal in Bahadur efficiency among all combined p-value methods. The Bahadur efficiency,[Formula: see text], compares sample sizes among different statistical tests when signals become sparse in sequencing data, i.e. ε →0. The optimal Bahadur efficiency ensures that the Lancaster procedure asymptotically requires a minimal sample size to detect sparse signals ([Formula: see text]. The Lancaster procedure can also be applied to meta-analysis. Extensive empirical assessments of exome sequencing data show that the proposed method outperforms Gene Set Enrichment Analysis (GSEA. We applied the competitive Lancaster procedure to meta-analysis data generated by the Global Lipids Genetics Consortium to identify pathways significantly associated with high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides, and total cholesterol.

  19. An Optimal Bahadur-Efficient Method in Detection of Sparse Signals with Applications to Pathway Analysis in Sequencing Association Studies.

    Science.gov (United States)

    Dai, Hongying; Wu, Guodong; Wu, Michael; Zhi, Degui

    2016-01-01

    Next-generation sequencing data pose a severe curse of dimensionality, complicating traditional "single marker-single trait" analysis. We propose a two-stage combined p-value method for pathway analysis. The first stage is at the gene level, where we integrate effects within a gene using the Sequence Kernel Association Test (SKAT). The second stage is at the pathway level, where we perform a correlated Lancaster procedure to detect joint effects from multiple genes within a pathway. We show that the Lancaster procedure is optimal in Bahadur efficiency among all combined p-value methods. The Bahadur efficiency,[Formula: see text], compares sample sizes among different statistical tests when signals become sparse in sequencing data, i.e. ε →0. The optimal Bahadur efficiency ensures that the Lancaster procedure asymptotically requires a minimal sample size to detect sparse signals ([Formula: see text]). The Lancaster procedure can also be applied to meta-analysis. Extensive empirical assessments of exome sequencing data show that the proposed method outperforms Gene Set Enrichment Analysis (GSEA). We applied the competitive Lancaster procedure to meta-analysis data generated by the Global Lipids Genetics Consortium to identify pathways significantly associated with high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides, and total cholesterol.

  20. Folic acid functionalized silver nanoparticles with sensitivity and selectivity colorimetric and fluorescent detection for Hg2+ and efficient catalysis.

    Science.gov (United States)

    Su, Dongyue; Yang, Xin; Xia, Qingdong; Zhang, Qi; Chai, Fang; Wang, Chungang; Qu, Fengyu

    2014-09-05

    In this research, folic acid functionalized silver nanoparticles (FA-AgNPs) were selected as a colorimetric and a 'turn on' fluorescent sensor for detecting Hg(2+). After being added into Hg(2+), AgNPs can emit stable fluorescence at 440 nm when the excitation wavelength is selected at 275 nm. The absorbance and fluorescence of the FA-AgNPs could reflect the concentration of the Hg(2+) ions. Thus, we developed a simple, sensitive analytical method to detect Hg(2+) based on the colorimetric and fluorescence enhancement of FA-AgNPs. The sensor exhibits two linear response ranges between absorbance and fluorescence intensity with Hg(2+) concentration, respectively. Meanwhile, a detection limit of 1 nM is estimated based on the linear relationship between responses with a concentration of Hg(2+). The high specificity of Hg(2+) with FA-AgNPs interactions provided the excellent selectivity towards detecting Hg(2+) over other metal ions (Pb(2+), Mg(2+), Zn(2+), Ni(2+), Cu(2+), Co(2+), Ca(2+), Mn(2+), Fe(2+), Cd(2+), Ba(2+), Cr(6+) and Cr(3+)). This will provide a simple, effective and multifunctional colorimetric and fluorescent sensor for on-site and real-time Hg(2+) ion detection. The proposed method can be applied to the analysis of trace Hg(2+) in lake water. Additionally, the FA-AgNPs can be used as efficient catalyst for the reduction of 4-nitrophenol and potassium hexacyanoferrate (III).

  1. Measurement of Rb Using a Vertex Mass Tag

    International Nuclear Information System (INIS)

    Steiner, R.; Benvenuti, A.C.; Coller, J.A.; Hedges, S.J.; Johnson, A.S.; Shank, J.T.; Whitaker, J.S.; Allen, N.J.; Cotton, R.; Dervan, P.J.; Hasan, A.; McKemey, A.K.; Watts, S.J.; Caldwell, D.O.; Lu, A.; Yellin, S.J.; Cavalli-Sforza, M.; Coyne, D.G.; Fernandez, J.P.; Liu, X.; Reinertsen, P.L.; Schalk, T.; Schumm, B.A.; DOliveira, A.; Johnson, R.A.; Meadows, B.T.; Nussbaum, M.; Dima, M.; Harton, J.L.; Smy, M.B.; Staengle, H.; Wilson, R.J.; Baranko, G.; Fahey, S.; Fan, C.; Krishna, N.M.; Lauber, J.A.; Nauenberg, U.; Wagner, D.L.; Bazarko, A.O.; Bolton, T.; Rowson, P.C.; Shaevitz, M.H.; Camanzi, B.; Mazzucato, E.; Piemontese, L.; Calcaterra, A.; De Sangro, R.; Peruzzi, I.; Piccolo, M.; Eisenstein, B.I.; Gladding, G.; Karliner, I.; Shapiro, G.; Steiner, H.; Bardon, O.; Burrows, P.N.; Busza, W.; Cowan, R.F.; Dong, D.N.; Fero, M.J.; Gonzalez, S.; Kendall, H.W.; Lath, A.; Lia, V.; Osborne, L.S.; Quigley, J.; Taylor, F.E.; Torrence, E.; Verdier, R.; Williams, D.C.

    1998-01-01

    We report a new measurement of R b =Γ Z 0 →bbar b /Γ Z 0 →hadrons using a double tag technique, where the b hemisphere selection is based on the reconstructed mass of the B hadron decay vertex. The measurement was performed using a sample of 130x10 3 hadronic Z 0 events, collected with the SLD detector at SLC. The method utilizes the 3D vertexing abilities of the CCD pixel vertex detector and the small stable SLC beams to obtain a high b -tagging efficiency and purity. We obtain R b =0.2142±0.0034(stat) ±0.0015(syst)±0.0002( R c ) . copyright 1998 The American Physical Society

  2. Tagged at first listen: an examination of social tagging practices in a music recommender system

    Directory of Open Access Journals (Sweden)

    Audrey Laplante

    2015-01-01

    Full Text Available http://dx.doi.org/10.5007/1518-2924.2015v20nesp1p33 Social tagging has become a very common way to index different types of resources on the web. Less prevalent in music than in other domains, social tagging is nevertheless used in a popular recommender system, Last.fm. Although the number of publications on tagging and folksonomies has exploded in the last few years, music tagging is still not well studied. In this paper, we present a study of tagging practices of Last.fm users. We examine the social tagging of songs during the first three months after their release. Our analysis shows that the release of a song triggers a burst in tagging activity that lasts two weeks, after what it decreases sharply and then remains fairly constant for the next ten weeks. We also find that a majority of songs do not get tagged during the first week and that tagging was positively related to popularity. Finally, we find that tags that have been frequently applied to a given song are more likely to be genre related, shorter in length, and relatively objective than tags that have been applied only once.

  3. Atom probe tomography evaporation behavior of C-axis GaN nanowires: Crystallographic, stoichiometric, and detection efficiency aspects

    International Nuclear Information System (INIS)

    Diercks, David R.; Gorman, Brian P.; Kirchhofer, Rita; Sanford, Norman; Bertness, Kris; Brubaker, Matt

    2013-01-01

    The field evaporation behavior of c-axis GaN nanowires was explored in two different laser-pulsed atom probe tomography (APT) instruments. Transmission electron microscopy imaging before and after atom probe tomography analysis was used to assist in reconstructing the data and assess the observed evaporation behavior. It was found that the ionic species exhibited preferential locations for evaporation related to the underlying crystal structure of the GaN and that the species which evaporated from these locations was dependent on the pulsed laser energy. Additionally, the overall stoichiometry measured by APT was significantly correlated with the energy of the laser pulses. At the lowest laser energies, the apparent composition was nitrogen-rich, while higher laser energies resulted in measurements of predominantly gallium compositions. The percent of ions detected (detection efficiency) for these specimens was found to be considerably below that shown for other materials, even for laser energies which produced the expected Ga:N ratio. The apparent stoichiometry variation and low detection efficiency appear to be a result of evaporation of Ga ions between laser pulses at the lowest laser energies and evaporation of neutral N 2 species at higher laser energies. All of these behaviors are tied to the formation of nitrogen-nitrogen bonds on the tip surface, which occurred under all analysis conditions. Similar field evaporation behaviors are therefore expected for other materials where the anionic species readily form a strong diatomic bond

  4. Efficient Structural System Reliability Updating with Subspace-Based Damage Detection Information

    DEFF Research Database (Denmark)

    Döhler, Michael; Thöns, Sebastian

    modelling is introduced building upon the non-destructive testing reliability which applies to structural systems and DDS containing a strategy to overcome the high computational efforts for the pre-determination of the DDS reliability. This approach takes basis in the subspace-based damage detection method......Damage detection systems and algorithms (DDS and DDA) provide information of the structural system integrity in contrast to e.g. local information by inspections or non-destructive testing techniques. However, the potential of utilizing DDS information for the structural integrity assessment...... and prognosis is hardly exploited nor treated in scientific literature up to now. In order to utilize the information provided by DDS for the structural performance, usually high computational efforts for the pre-determination of DDS reliability are required. In this paper, an approach for the DDS performance...

  5. An Efficient Integrated Approach for the Detection of Exudates and Diabetic Maculopathy in Colour fundus Images

    OpenAIRE

    B.Ramasubramanian; G.Mahendran

    2012-01-01

    Diabetic Retinopathy (DR) is a major cause of blindness. Exudates are one of the primary signs ofdiabetic retinopathy which is a main cause of blindness that could be prevented with an early screeningprocess In this approach, the process and knowledge of digital image processing to diagnose exudatesfrom images of retina is applied. An automated method to detect and localize the presence of exudatesand Maculopathy from low-contrast digital images of Retinopathy patient’s with non-dilated pupil...

  6. PHACK: An Efficient Scheme for Selective Forwarding Attack Detection in WSNs

    Directory of Open Access Journals (Sweden)

    Anfeng Liu

    2015-12-01

    Full Text Available In this paper, a Per-Hop Acknowledgement (PHACK-based scheme is proposed for each packet transmission to detect selective forwarding attacks. In our scheme, the sink and each node along the forwarding path generate an acknowledgement (ACK message for each received packet to confirm the normal packet transmission. The scheme, in which each ACK is returned to the source node along a different routing path, can significantly increase the resilience against attacks because it prevents an attacker from compromising nodes in the return routing path, which can otherwise interrupt the return of nodes’ ACK packets. For this case, the PHACK scheme also has better potential to detect abnormal packet loss and identify suspect nodes as well as better resilience against attacks. Another pivotal issue is the network lifetime of the PHACK scheme, as it generates more acknowledgements than previous ACK-based schemes. We demonstrate that the network lifetime of the PHACK scheme is not lower than that of other ACK-based schemes because the scheme just increases the energy consumption in non-hotspot areas and does not increase the energy consumption in hotspot areas. Moreover, the PHACK scheme greatly simplifies the protocol and is easy to implement. Both theoretical and simulation results are given to demonstrate the effectiveness of the proposed scheme in terms of high detection probability and the ability to identify suspect nodes.

  7. Computationally efficient algorithm for photoplethysmography-based atrial fibrillation detection using smartphones.

    Science.gov (United States)

    Schack, Tim; Safi Harb, Yosef; Muma, Michael; Zoubir, Abdelhak M

    2017-07-01

    Atrial fibrillation (AF) is one of the major causes of stroke, heart failure, sudden death, and cardiovascular morbidity and the most common type of arrhythmia. Its diagnosis and the initiation of treatment, however, currently requires electrocardiogram (ECG)-based heart rhythm monitoring. The photoplethysmogram (PPG) offers an alternative method, which is convenient in terms of its recording and allows for self-monitoring, thus relieving clinical staff and enabling early AF diagnosis. We introduce a PPG-based AF detection algorithm using smartphones that has a low computational cost and low memory requirements. In particular, we propose a modified PPG signal acquisition, explore new statistical discriminating features and propose simple classification equations by using sequential forward selection (SFS) and support vector machines (SVM). The algorithm is applied to clinical data and evaluated in terms of receiver operating characteristic (ROC) curve and statistical measures. The combination of Shannon entropy and the median of the peak rise height achieves perfect detection of AF on the recorded data, highlighting the potential of PPG for reliable AF detection.

  8. Detection Efficiency of Microcalcification using Computer Aided Diagnosis in the Breast Ultrasonography Images

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Jin Soo; Ko, Seong Jin; Kang, Se Sik; Kim, Jung Hoon; Choi, Seok Yoon; Kim, Chang Soo [Dept. of Radiological Science, Catholic University of Pusan, Pusan (Korea, Republic of); Park, Hyung Hu [Dept. of Health Science, Graduate School of Kosin University, Pusan (Korea, Republic of)

    2012-09-15

    Digital Mammography makes it possible to reproduce the entire breast image. And it is used to detect microcalcification and mass which are the most important point of view of nonpalpable early breast cancer, so it has been used as the primary screening test of breast disease. It is reported that microcalcification of breast lesion is important in diagnosis of early breast cancer. In this study, six types of texture features algorithms are used to detect microcalcification on breast US images and the study has analyzed recognition rate of lesion between normal US images and other US images which microcalification is seen. As a result of the experiment, Computer aided diagnosis recognition rate that distinguishes mammography and breast US disease was considerably high 70-98%. The average contrast and entropy parameters were low in ROC analysis, but sensitivity and specificity of four types parameters were over 90%. Therefore it is possible to detect microcalcification on US images. If not only six types of texture features algorithms but also the research of additional parameter algorithm is being continually proceeded and basis of practical use on CAD is being prepared, it can be a important meaning as pre-reading. Also, it is considered very useful things for early diagnosis of breast cancer.

  9. Efficient and automatic image reduction framework for space debris detection based on GPU technology

    Science.gov (United States)

    Diprima, Francesco; Santoni, Fabio; Piergentili, Fabrizio; Fortunato, Vito; Abbattista, Cristoforo; Amoruso, Leonardo

    2018-04-01

    In the last years, the increasing number of space debris has triggered the need of a distributed monitoring system for the prevention of possible space collisions. Space surveillance based on ground telescope allows the monitoring of the traffic of the Resident Space Objects (RSOs) in the Earth orbit. This space debris surveillance has several applications such as orbit prediction and conjunction assessment. In this paper is proposed an optimized and performance-oriented pipeline for sources extraction intended to the automatic detection of space debris in optical data. The detection method is based on the morphological operations and Hough Transform for lines. Near real-time detection is obtained using General Purpose computing on Graphics Processing Units (GPGPU). The high degree of processing parallelism provided by GPGPU allows to split data analysis over thousands of threads in order to process big datasets with a limited computational time. The implementation has been tested on a large and heterogeneous images data set, containing both imaging satellites from different orbit ranges and multiple observation modes (i.e. sidereal and object tracking). These images were taken during an observation campaign performed from the EQUO (EQUatorial Observatory) observatory settled at the Broglio Space Center (BSC) in Kenya, which is part of the ASI-Sapienza Agreement.

  10. A Compact Dual-Band RFID Tag Antenna Mountable on Metallic Objects

    Directory of Open Access Journals (Sweden)

    Byeonggwi Mun

    2015-01-01

    Full Text Available A compact (50 × 50 × 4 mm3 dual-band radio frequency identification (RFID tag antenna mountable on metallic objects is proposed for the ultra-high frequency (UHF band (917∼923.5 MHz and the microwave (MW band (2.4∼2.45 GHz. With the proximity-coupled feed loop, the proposed antenna consists of two symmetric planar inverted-F antenna (PIFA elements for the UHF band passive tag and a meander microstrip patch antenna for the MW band active tag. The performance of the proposed antenna is verified by mounting it on the different sizes of the metallic object. Furthermore, the passive tag antenna in the UHF band furthermore may be used for energy harvesting techniques to improve the lifetime of the active tag in the MW band. The measured maximum read range is 5.50 m in the UHF band and 14.15 m in the MW band when the proposed tag antenna is mounted on the metallic objects. The total efficiency for all operating frequency bands is higher than 50%. High isolation (>12 dB between tag antennas in the UHF band and the MW band is achieved.

  11. Performance and Benchmarking of Multisurface UHF RFID Tags for Readability and Reliability

    Directory of Open Access Journals (Sweden)

    Joshua Bolton

    2017-01-01

    Full Text Available As the price of passive radio frequency identification (RFID tags continues to decrease, more and more companies are considering item-level tagging. Although the use of RFID is simple, its proper application should be studied to achieve maximum efficiency and utilization in the industry. This paper is intended to demonstrate the test results of various multisurface UHF tags from different manufacturers for their readability under varying conditions such as orientation of tags with respect to reader, distance of tag from the reader, and materials used for embedding tags. These conditions could affect the reliability of RFID systems used for varied applications. In this paper, we implement a Design for Six Sigma Research (DFSS-R methodology that allows for reliability testing of RFID systems. In this paper, we have showcased our results about the benchmarking of UHF RFID tags and have put forward an important observation about the blind spots observed at different distances and orientations along different surfaces, which is primarily due to the polarity of the antenna chosen.

  12. Improvements in Boron Plate Coating Technology for Higher Efficiency Neutron Detection and Coincidence Counting Error Reduction

    Energy Technology Data Exchange (ETDEWEB)

    Menlove, Howard Olsen [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Henzlova, Daniela [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-08-25

    This informal report presents the measurement data and information to document the performance of the advanced Precision Data Technology, Inc. (PDT) sealed cell boron-10 plate neutron detector that makes use of the advanced coating materials and procedures. In 2015, PDT changed the boron coating materials and application procedures to significantly increase the efficiency of their basic corrugated plate detector performance. A prototype sealed cell unit was supplied to LANL for testing and comparison with prior detector cells. Also, LANL had reference detector slabs from the original neutron collar (UNCL) and the new Antech UNCL with the removable 3He tubes. The comparison data is presented in this report.

  13. Detection efficiency for radionuclides decaying by electron capture and gamma-Ray

    International Nuclear Information System (INIS)

    Grau, A.; Fernandez, A.

    1985-01-01

    In this paper, the electron capture partial counting efficiency vs the figure of merit for electron-capture and gamma-ray emitters has been computed. The radionuclides tabulated are 48 c r, 54 M n, 57 C o 56 N i, 72 S e, 73 A s, 85 S r, 88 Z r, 92 N b, 103 P d, 111 l n, 119 S b, 125 I , 139 C e and 152 D y. It has been assumed that the liquid is a toluene based scintillator solution in standard glass vials containing 15 cm 3 . (Author) 17 refs

  14. Neutron sensitivity and detection efficiency of 3He and 10BF3 counters

    International Nuclear Information System (INIS)

    Khokonov, A. Kh.; Savoiskii, Yu. V.; Kamarzaev, A. V.

    2010-01-01

    An analytic relationship between the efficiency, sensitivity, and the coefficient of working-isotope utilization is established for gas-filled cylindrical thermal-neutron counters. This dependence is expressed in terms of the ratio of the characteristic length of a counter to its diameter. The characteristic size is introduced as the ratio of the total absorption cross section to the length of the counter. The parameters of basic 3 He and 10 BF 3 counters used in NM-64 neutron monitors are calculated.

  15. CT colonography with rectal iodine tagging: Feasibility and comparison with oral tagging in a colorectal cancer screening population.

    Science.gov (United States)

    Neri, Emanuele; Mantarro, Annalisa; Faggioni, Lorenzo; Scalise, Paola; Bemi, Pietro; Pancrazi, Francesca; D'Ippolito, Giuseppe; Bartolozzi, Carlo

    2015-09-01

    To evaluate feasibility, diagnostic performance, patient acceptance, and overall examination time of CT colonography (CTC) performed through rectal administration of iodinated contrast material. Six-hundred asymptomatic subjects (male:female=270:330; mean 63 years) undergoing CTC for colorectal cancer screening on an individual basis were consecutively enrolled in the study. Out of them, 503 patients (group 1) underwent CTC with rectal tagging, of which 55 had a total of 77 colonic lesions. The remaining 97 patients (group 2) were randomly selected to receive CTC with oral tagging of which 15 had a total of 20 colonic lesions. CTC findings were compared with optical colonoscopy, and per-segment image quality was visually assessed using a semi-quantitative score (1=poor, 2=adequate, 3=excellent). In 70/600 patients (11.7%), CTC was performed twice with both types of tagging over a 5-year follow-up cancer screening program. In this subgroup, patient acceptance was rated via phone interview two weeks after CTC using a semi-quantitative scale (1=poor, 2=fair, 3=average, 4=good, 5=excellent). Mean per-polyp sensitivity, specificity, positive and negative predictive values of CTC with rectal vs oral tagging were 96.1% (CI95% 85.4÷99.3%) vs 89.4% (CI95% 65.4÷98.1%), 95.3% (CI95% 90.7÷97.8%) vs 95.8% (CI95% 87.6÷98.9%), 86.0% (CI95% 73.6÷93.3) vs 85.0% (CI95% 61.1÷96.0%), and 98.8% (CI95% 95.3÷99.8%) vs 97.2% (CI95% 89.4÷99.5%), respectively (p>0.05). Polyp detection rates were not statistically different between groups 1 and 2 (p>0.05). Overall examination time was significantly shorter with rectal than with oral tagging (18.3±3.5 vs 215.6±10.3 minutes, respectively; pRectal iodine tagging can be an effective alternative to oral tagging for CTC with the advantages of greater patient acceptance and lower overall examination time. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  16. Pop-up Archival Transmitting (PAT) fish tag data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The cooperative tagging center (CTC) began deploying electronic tags in 2002. To date over 300 tags have been deployed. The following species have been monitored:...

  17. Energy resolution and efficiency of phonon-mediated kinetic inductance detectors for light detection

    Energy Technology Data Exchange (ETDEWEB)

    Cardani, L., E-mail: laura.cardani@roma1.infn.it [Dipartimento di Fisica, Sapienza Università di Roma, Piazzale Aldo Moro 2, 00185 Roma (Italy); Physics Department, Princeton University, Washington Road, 08544, Princeton, New Jersey (United States); Colantoni, I.; Coppolecchia, A. [Dipartimento di Fisica, Sapienza Università di Roma, Piazzale Aldo Moro 2, 00185 Roma (Italy); Cruciani, A.; Vignati, M.; Bellini, F.; Casali, N.; Cosmelli, C. [Dipartimento di Fisica, Sapienza Università di Roma, Piazzale Aldo Moro 2, 00185 Roma (Italy); INFN - Sezione di Roma, Piazzale Aldo Moro 2, 00185 Roma (Italy); Di Domizio, S. [Dipartimento di Fisica, Università degli Studi di Genova, Via Dodecaneso 33, 16146 Genova (Italy); INFN - Sezione di Genova, Via Dodecaneso 33, 16146 Genova (Italy); Castellano, M. G. [Istituto di Fotonica e Nanotecnologie - CNR, Via Cineto Romano 42, 00156 Roma (Italy); Tomei, C. [INFN - Sezione di Roma, Piazzale Aldo Moro 2, 00185 Roma (Italy)

    2015-08-31

    The development of sensitive cryogenic light detectors is of primary interest for bolometric experiments searching for rare events like dark matter interactions or neutrino-less double beta decay. Thanks to their good energy resolution and the natural multiplexed read-out, Kinetic Inductance Detectors (KIDs) are particularly suitable for this purpose. To efficiently couple KIDs-based light detectors to the large crystals used by the most advanced bolometric detectors, active surfaces of several cm{sup 2} are needed. For this reason, we are developing phonon-mediated detectors. In this paper, we present the results obtained with a prototype consisting of four 40 nm thick aluminum resonators patterned on a 2 × 2 cm{sup 2} silicon chip, and calibrated with optical pulses and X-rays. The detector features a noise resolution σ{sub E} = 154 ± 7 eV and an (18 ± 2)% efficiency.

  18. Efficient induction of Wheat-agropyron cristatum 6P translocation lines and GISH detection.

    Directory of Open Access Journals (Sweden)

    Liqiang Song

    Full Text Available The narrow genetic background restricts wheat yield and quality improvement. The wild relatives of wheat are the huge gene pools for wheat improvement and can broaden its genetic basis. Production of wheat-alien translocation lines can transfer alien genes to wheat. So it is important to develop an efficient method to induce wheat-alien chromosome translocation. Agropyroncristatum (P genome carries many potential genes beneficial to disease resistance, stress tolerance and high yield. Chromosome 6P possesses the desirable genes exhibiting good agronomic traits, such as high grain number per spike, powdery mildew resistance and stress tolerance. In this study, the wheat-A. cristatum disomic addition was used as bridge material to produce wheat-A. cristatum translocation lines induced by (60Co-γirradiation. The results of genomic in situ hybridization showed that 216 plants contained alien chromosome translocation among 571 self-pollinated progenies. The frequency of translocation was 37.83%, much higher than previous reports. Moreover, various alien translocation types were identified. The analysis of M2 showed that 62.5% of intergeneric translocation lines grew normally without losing the translocated chromosomes. The paper reported a high efficient technical method for inducing alien translocation between wheat and Agropyroncristatum. Additionally, these translocation lines will be valuable for not only basic research on genetic balance, interaction and expression of different chromosome segments of wheat and alien species, but also wheat breeding programs to utilize superior agronomic traits and good compensation effect from alien chromosomes.

  19. Double positive effect of adding hexaethyelene glycol when optimizing the hybridization efficiency of a microring DNA detection assay

    Energy Technology Data Exchange (ETDEWEB)

    Van Eeghem, Anabelle, E-mail: anabelle.vaneeghem@gmail.com [Polymer Chemistry and Biomaterials Research Group, Department of Organic and Macromolecular Chemistry, Ghent University (Belgium); Center for Nano- and Biophotonics, Ghent University (Belgium); Werquin, Sam [Center for Nano- and Biophotonics, Ghent University (Belgium); Photonics Research Group, Department of Information Technology, Ghent University – IMEC (Belgium); Hoste, Jan-Willem, E-mail: janwillem.hoste@ugent.be [Center for Nano- and Biophotonics, Ghent University (Belgium); Photonics Research Group, Department of Information Technology, Ghent University – IMEC (Belgium); Goes, Arne [Polymer Chemistry and Biomaterials Research Group, Department of Organic and Macromolecular Chemistry, Ghent University (Belgium); Agrosavfe NV, Technologiepark 4 (Bio-incubator), Zwijnaarde (Belgium); Vanderleyden, Els [Polymer Chemistry and Biomaterials Research Group, Department of Organic and Macromolecular Chemistry, Ghent University (Belgium); Center for Nano- and Biophotonics, Ghent University (Belgium); Bienstman, Peter [Center for Nano- and Biophotonics, Ghent University (Belgium); Photonics Research Group, Department of Information Technology, Ghent University – IMEC (Belgium); Dubruel, Peter [Polymer Chemistry and Biomaterials Research Group, Department of Organic and Macromolecular Chemistry, Ghent University (Belgium); Center for Nano- and Biophotonics, Ghent University (Belgium)

    2017-05-31

    Highlights: • The hybridization efficiency of a DNA assay was investigated based on SOI microring resonators. • A 4-fold increase in efficiency was obtained by using HEG as backfilling agent, as well as improving robustness. • The dual polarization microring technique shows that HEG reorients the DNA in an upright position. • Hybridizing at 35 °C and with a buffer containing 50 v/v% of formamide greatly improves the robustness. - Abstract: In this paper, a method for detection of DNA molecules using silicon-on-insulator (SOI) microring resonators is described. The influence of temperature and the use of formamide on the hybridization efficiency were studied. It was shown that 50 v/v% of formamide in the hybridization buffer can ensure hybridization when working close to physiological temperature. Furthermore, the use of hexaethylene glycol (HEG) as backfilling agent was studied in order to resolve issues of non-specific adsorption to the surface. The results indicated that not only non-specific binding was reduced significantly but also that HEG improves the orientation of the DNA probes on the surface. This led to a 4-fold increase in hybridization efficiency and thus in an equal decrease in the detection limit, compared to hybridization without the use of HEG. An improvement in robustness of the assay was also observed. This DNA reorientation hypothesis was confirmed by studying the thickness and density of the layers by using dual polarization microring sensing. Finally, the different steps in the sensing experiment were characterized in more detail by static contact angle (SCA) and X-ray photoelectron spectroscopy (XPS) analysis. The results showed quantitatively that the surface modifications were successful.

  20. Assessing Nitrogen Treatment Efficiency in Schima Superba Seedlings Detected Using Hyperspectral Reflectance

    Directory of Open Access Journals (Sweden)

    Miaomiao Cheng

    2014-01-01

    Full Text Available The sharp change in nitrate (N deposition fluxes due to anthropogenic influences has major consequences for terrestrial plant productivity. Early detection of plants under nitrate stress is important for forest management in the subtropical region. This study used leaf-scale hyperspectral reflectance measurements to detect the seedling growth response of Schima superba (S. superba under simulated N deposition during a period of two years. Two-year-old S. superba seedlings were planted under natural field conditions and treated with four N treatments at CK, LN-6, MN-10, and HN-24g N m-2 year-1. The chlorophyll content and leaf reflectance were examined to detect the N addition temporal effects. Results indicated that S. superba responded significantly with differences in chlorophyll content and leaf reflectance to N additional treatment. Compared with the N deficiency (CK plots, plots with higher N addition rate (HN reduced the chlorophyll concentration of S. superba seedlings. However, the long-term observed impact of LN and MN treatments increased the S. superba chlorophyll during the two years. Nitrogen additional treatments can be distinguished using the hyperspectral indices (R700/R720, R695/R420, and R695/R760 retrieved from the differences in leaf reflectance at the green spectrum and the red spectrum. The derivative shift to longer wavelength peaks with increasing N supply, accompanied by the increase in chlorophyll content. Leaf reflectance at 559 nm was negatively correlated with leaf chlorophyll content (R = -0.77. The identified N specific spectral ratios may be used for image interpretation and plant N status diagnosis for site-specific N management.

  1. Fluorescence detection using optical waveguide collection device with high efficiency on assembly of nitrogen vacancy centers in diamond

    Science.gov (United States)

    Zhang, Shaowen; Ma, Zongmin; Qin, Li; Fu, Yueping; Shi, Yunbo; Liu, Jun; Li, Yan Jun

    2018-01-01

    In this letter, we propose a fluorescence waveguide excitation and collection (FWEC) method that allows for an excess of 45% collection efficiency of pump photons into optically detected magnetic resonance. The FWEC system used can collect fluorescence 96 times higher than the confocal system under spin manipulation with a microwave. Furthermore, the signal-to-noise ratio (SNR) of the FWEC system is improved 9 times compared with that of the confocal system. In addition, the increase in contrast observed using the FWEC system shows that the integration of the system is much improved with 3D printing technology. Thus, this research has a great potential application in subsequent magnetic detection and quantum optics.

  2. Computational efficient unsupervised coastline detection from single-polarization 1-look SAR images of complex coastal environments

    Science.gov (United States)

    Garzelli, Andrea; Zoppetti, Claudia; Pinelli, Gianpaolo

    2017-10-01

    Coastline detection in synthetic aperture radar (SAR) images is crucial in many application fields, from coastal erosion monitoring to navigation, from damage assessment to security planning for port facilities. The backscattering difference between land and sea is not always documented in SAR imagery, due to the severe speckle noise, especially in 1-look data with high spatial resolution, high sea state, or complex coastal environments. This paper presents an unsupervised, computationally efficient solution to extract the coastline acquired by only one single-polarization 1-look SAR image. Extensive tests on Spotlight COSMO-SkyMed images of complex coastal environments and objective assessment demonstrate the validity of the proposed procedure which is compared to state-of-the-art methods through visual results and with an objective evaluation of the distance between the detected and the true coastline provided by regional authorities.

  3. Detecting and Preventing Beacon Replay Attacks in Receiver-Initiated MAC Protocols for Energy Efficient WSNs

    DEFF Research Database (Denmark)

    Di Mauro, Alessio; Fafoutis, Xenofon; Mödersheim, Sebastian Alexander

    2013-01-01

    nodes, pretending to have a fake identity within the network. To prevent this attack we propose RAP, a challenge-response authentication protocol that is able to detect and prevent the beacon replay attack. The effectiveness of the protocol is formally verified using OFMC and ProVerif. Furthermore, we......In receiver-initiated MAC protocols for Wireless Sensor Networks (WSNs), communication is initiated by the receiver of the data through beacons containing the receiver's identity. In this paper, we consider the case of a network intruder that captures and replays such beacons towards legitimate...

  4. VIBRATIONS DETECTION IN INDUSTRIAL PUMPS BASED ON SPECTRAL ANALYSIS TO INCREASE THEIR EFFICIENCY

    Directory of Open Access Journals (Sweden)

    Belhadef RACHID

    2016-01-01

    Full Text Available Spectral analysis is the key tool for the study of vibration signals in rotating machinery. In this work, the vibration analy-sis applied for conditional preventive maintenance of such machines is proposed, as part of resolved problems related to vibration detection on the organs of these machines. The vibration signal of a centrifugal pump was treated to mount the benefits of the approach proposed. The obtained results present the signal estimation of a pump vibration using Fourier transform technique compared by the spectral analysis methods based on Prony approach.

  5. A minimally invasive multiple marker approach allows highly efficient detection of meningioma tumors

    Directory of Open Access Journals (Sweden)

    Meese Eckart

    2006-12-01

    Full Text Available Abstract Background The development of effective frameworks that permit an accurate diagnosis of tumors, especially in their early stages, remains a grand challenge in the field of bioinformatics. Our approach uses statistical learning techniques applied to multiple antigen tumor antigen markers utilizing the immune system as a very sensitive marker of molecular pathological processes. For validation purposes we choose the intracranial meningioma tumors as model system since they occur very frequently, are mostly benign, and are genetically stable. Results A total of 183 blood samples from 93 meningioma patients (WHO stages I-III and 90 healthy controls were screened for seroreactivity with a set of 57 meningioma-associated antigens. We tested several established statistical learning methods on the resulting reactivity patterns using 10-fold cross validation. The best performance was achieved by Naïve Bayes Classifiers. With this classification method, our framework, called Minimally Invasive Multiple Marker (MIMM approach, yielded a specificity of 96.2%, a sensitivity of 84.5%, and an accuracy of 90.3%, the respective area under the ROC curve was 0.957. Detailed analysis revealed that prediction performs particularly well on low-grade (WHO I tumors, consistent with our goal of early stage tumor detection. For these tumors the best classification result with a specificity of 97.5%, a sensitivity of 91.3%, an accuracy of 95.6%, and an area under the ROC curve of 0.971 was achieved using a set of 12 antigen markers only. This antigen set was detected by a subset selection method based on Mutual Information. Remarkably, our study proves that the inclusion of non-specific antigens, detected not only in tumor but also in normal sera, increases the performance significantly, since non-specific antigens contribute additional diagnostic information. Conclusion Our approach offers the possibility to screen members of risk groups as a matter of routine

  6. Numerical Study of Plasmonic Efficiency of Gold Nanostripes for Molecule Detection

    Directory of Open Access Journals (Sweden)

    Thomas Grosges

    2015-01-01

    Full Text Available In plasmonics, the accurate computation of the electromagnetic field enhancement is necessary in determining the amplitude and the spatial extension of the field around nanostructures. Here, the problem of the interaction between an electromagnetic excitation and gold nanostripes is solved. An optimization scheme, including an adaptive remeshing process with error estimator, is used to solve the problem through a finite element method. The variations of the electromagnetic field amplitude and the plasmonic active zones around nanostructures for molecule detection are studied in this paper taking into account the physical and geometrical parameters of the nanostripes. The evolution between the sizes and number of nanostripes is shown.

  7. Improved Detection Technique for the Uncoded MIMO Systems Using an Efficient K-best Algorithm.

    OpenAIRE

    Hellassa Wassim; Boukari Karima

    2015-01-01

    This paper presents a new algorithm for spatially multiplexed MIMO detection that exploits the resources available in the search procedure to enhance the error performance of the K-best approach of search. The proposed algorithm exploits the heuristics of the nodes and it is referred as the Heuristic K-best algorithm (HK-best). Unlike the conventional K-best algorithm, the HK-best algorithm sort the visited nodes in each layer based on the path metric and the heuristic. Simulation results sho...

  8. Design and testing of RFID sensor tag fabricated using inkjet-printing and electrodeposition

    Science.gov (United States)

    Chien Dang, Mau; Son Nguyen, Dat; Dung Dang, Thi My; Tedjini, Smail; Fribourg-Blanc, Eric

    2014-06-01

    The passive RFID tag with an added sensing function is of interest to many applications. In particular, applications where RFID tagging is already considered to be the next step, such as food items, are a specific target. This paper demonstrates a flexible RFID tag sensor fabricated using a low cost technique with an added zero-cost sensing function. It is more specifically applied to the sensing of degradable food, in particular beef meat in our demonstrated example. To reach this, the antenna is designed in such a way to be sensitive to the variation of the dielectric permittivity of the meat over time. The design of the sensing tag as well as its fabrication process are described. The fabrication involves inkjet printing of a silver nanoparticle based ink on a commercial low cost PET film to create a seed layer. It is followed by a copper electrodeposition step on top of the silver pattern to complete the tag to obtain the desired thickness and conductivity of the tag antenna. The results of the electrical tests showed that with the inkjet printing-electrodeposition combination it is possible to produce flexible electrically conductive patterns for practical RFID applications. The tag was then tested in close-to-real-world conditions and it is demonstrated that it can provide a sensing function to detect the consumption limit of the packaged beef.

  9. Enhanced UHF RFID tags for drug tracing.

    Science.gov (United States)

    Catarinucci, Luca; Colella, Riccardo; De Blasi, Mario; Patrono, Luigi; Tarricone, Luciano

    2012-12-01

    Radio Frequency Identification (RFID) technology is playing a crucial role for item-level tracing systems in healthcare scenarios. The pharmaceutical supply chain is a fascinating application context, where RFID can guarantee transparency in the drug flow, supporting both suppliers and consumers against the growing counterfeiting problem. In such a context, the choice of the most adequate RFID tag, in terms of shape, frequency, size and reading range, is crucial. The potential presence of items containing materials hostile to the electromagnetic propagation exasperates the problem. In addition, the peculiarities of the different RFID-based checkpoints make even more stringent the requirements for the tag. In this work, the performance of several commercial UHF RFID tags in each step of the pharmaceutical supply chain has been evaluated, confirming the expected criticality. On such basis, a guideline for the electromagnetic design of new high-performance tags capable to overcome such criticalities has been defined. Finally, driven by such guidelines, a new enhanced tag has been designed, realized and tested. Due to patent pending issues, the antenna shape is not shown. Nevertheless, the optimal obtained results do not lose their validity. Indeed, on the one hand they demonstrate that high performance item level tracing systems can actually be implemented also in critical operating conditions. On the other hand, they encourage the tag designer to follow the identified guidelines so to realize enhanced UHF tags.

  10. Comparison of four PCR methods for efficient detection of Trypanosoma cruzi in routine diagnostics.

    Science.gov (United States)

    Seiringer, Peter; Pritsch, Michael; Flores-Chavez, María; Marchisio, Edoardo; Helfrich, Kerstin; Mengele, Carolin; Hohnerlein, Stefan; Bretzel, Gisela; Löscher, Thomas; Hoelscher, Michael; Berens-Riha, Nicole

    2017-07-01

    Due to increased migration, Chagas disease has become an international health problem. Reliable diagnosis of chronically infected people is crucial for prevention of non-vectorial transmission as well as treatment. This study compared four distinct PCR methods for detection of Trypanosoma cruzi DNA for the use in well-equipped routine diagnostic laboratories. DNA was extracted of T. cruzi-positive and negative patients' blood samples and cultured T. cruzi, T. rangeli as well as Leishmania spp. One conventional and two real-time PCR methods targeting a repetitive Sat-DNA sequence as well as one conventional PCR method targeting the variable region of the kDNA minicircle were compared for sensitivity, intra- and interassay precision, limit of detection, specificity and cross-reactivity. Considering the performance, costs and ease of use, an algorithm for PCR-diagnosis of patients with a positive serology for T. cruzi antibodies was developed. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

  11. Near-infrared phosphorescent polymeric nanomicelles: efficient optical probes for tumor imaging and detection.

    Science.gov (United States)

    Kumar, Rajiv; Ohulchanskyy, Tymish Y; Roy, Indrajit; Gupta, Sandesh K; Borek, Carsten; Thompson, Mark E; Prasad, Paras N

    2009-07-01

    We report a formulation of near-infrared (near-IR) phosphorescent polymeric nanomicelles and their use for in vivo high-contrast optical imaging, targeting, and detection of tumors in small animals. Near-IR phosphorescent molecules of Pt(II)-tetraphenyltetranaphthoporphyrin (Pt(TPNP)) were found to maintain their near-IR phosphorescence properties when encapsulated into phospholipid nanomicelles. The prepared phosphorescent micelles are of approximately 100 nm size and are highly stable in aqueous suspensions. A large spectral separation between the Pt(TPNP) absorption, with a peak at approximately 700 nm, and its phosphorescence emission, with a peak at approximately 900 nm, allows a dramatic decrease in the level of background autofluorescence and scattered excitation light in the near-IR spectral range, where the signal from the phosphorescent probe is observed. In vivo animal imaging with subcutaneously xenografted tumor-bearing mice has resulted in high contrast optical images, indicating highly specific accumulation of the phosphorescent micelles into tumors. Using optical imaging with near-IR phosphorescent nanomicelles, detection of smaller, visually undetectable tumors has also been demonstrated.

  12. Near infrared phosphorescent polymeric nanomicelles: efficient optical probes for tumor imaging and detection

    Science.gov (United States)

    Kumar, Rajiv; Ohulchanskyy, Tymish Y.; Roy, Indrajit; Gupta, Sandesh K.; Borek, Carsten; Thompson, Mark E.; Prasad, Paras N.

    2013-01-01

    We report a formulation of near infrared (NIR) phosphorescent polymeric nanomicelles and their use for in vivo high contrast optical imaging, targeting and detection of tumors in small animals. NIR phosphorescent molecules of Pt(II)-tetraphenyltetranaphthoporphyrin [Pt(TPNP)] were found to maintain their NIR phosphorescence properties when encapsulated into phospholipid nanomicelles. The prepared phosphorescent micelles are of ~100 nm size and are highly stable in aqueous suspensions. A large spectral separation between Pt(TPNP) absorption, peaked at ~700 nm, and its phosphorescence emission, with peak at ~ 900 nm, allows a dramatic decrease in the level of background autofluorescence and scattered excitation light in the NIR spectral range, where the signal from phosphorescent probe is observed. In vivo animal imaging with subcutaneously xenograted tumor-bearing mice has resulted in high contrast optical images, indicating highly specific accumulation of the phosphorescent micelles into tumors. Using optical imaging with NIR phosphorescent nanomicelles, detection of smaller, visually undetectable tumors has also been demonstrated. PMID:20355951

  13. Efficient DSA-DP hybrid lithography conflict detection and guiding template assignment

    Science.gov (United States)

    Ou, Jiaojiao; Cline, Brian; Yeric, Greg; Pan, David Z.

    2017-03-01

    In recent years, directed self-assembly (DSA) has demonstrated tremendous potential to reduce cost for multiple patterning with fewer masks, especially for via patterning. DSA is considered as one of the next generation lithography candidates or complementary lithography techniques to extend 193i lithography further for the sub- 7 nm nodes. In this work, we focus on the simultaneous DSA guiding template assignment and decomposition with DSA and double patterning (DSA-DP) hybrid lithography for 7nm technology node. We first analyze the placement error of DSA patterns with different shapes and sizes. We then propose a graph-based approach to reduce the problem size and solve the problem more efficiently without affecting the optimality of the results. The experimental results demonstrate that we can achieve a 50% reduction in both the number of variables and constraints compared to previous work, which leads to a 50X speed up in runtime.

  14. Evaluation of the detection efficiency of an x-ray image sensor

    International Nuclear Information System (INIS)

    Wulveryck, J.M.; Mouze, D.

    2000-01-01

    This work deals with the measurements of the spectral efficiency of an x-ray camera by a straightforward method, using laboratory x-ray sources. By mean of a fluorescence wavelength dispersive spectrometer (WDS), we carry out measurements in a large energy range, typically from a few keV to tenths of keV. The camera consists of a slow scan transfer device (CCD) optically coupled to a scintillator screen (Y 2 O 2 S:Eu). The spectral responsivity of the x-ray imaging detector - in ADU/keV units - is discussed. In particularly, the assumption of a linear conversion from incident x-ray energy to visible photon energy is confirmed by the experiment

  15. Communication methods, systems, apparatus, and devices involving RF tag registration

    Science.gov (United States)

    Burghard, Brion J [W. Richland, WA; Skorpik, James R [Kennewick, WA

    2008-04-22

    One technique of the present invention includes a number of Radio Frequency (RF) tags that each have a different identifier. Information is broadcast to the tags from an RF tag interrogator. This information corresponds to a maximum quantity of tag response time slots that are available. This maximum quantity may be less than the total number of tags. The tags each select one of the time slots as a function of the information and a random number provided by each respective tag. The different identifiers are transmitted to the interrogator from at least a subset of the RF tags.

  16. b-tagging performance at 13 TeV for the ATLAS experiment

    International Nuclear Information System (INIS)

    Lapertosa, A.

    2017-01-01

    The correct identification of jets containing b hadrons (hence b-tagging) is of capital importance for the hadron collider experiments, such as ATLAS and CMS at the Large Hadron Collider. In particular, b-tagging is an important tool for many physics analyses: for top quark studies and Higgs boson searches, as well as the search for new physics fenomena beyond Standard Model. ATLAS developed its own algorithms for b-jet identification, exploiting the typical properties of the b quarks and the B hadrons emerging from the jet: the long lifetime, the high decay multiplicity and the high invariant mass above all. The status of the most recent b-tagging algorithms developed by the ATLAS experiment is presented, along with the methods used to measure the b-tagging efficiency on a charm jets sample at 13TeV with 2015 data.

  17. 3.56-bits/cm Compact Inkjet Printed and Application Specific Chipless RFID Tag

    KAUST Repository

    Khan, Munawar M.

    2015-10-26

    © 2002-2011 IEEE. In this letter, a 28.5-bit chipless RFID tag, based on paper substrate and realized using inkjet printing technique is presented. Operating within ultrawideband, the tag occupies a compact size of 2 × 4 cm2. Focusing on applications requiring time and date identification, a novel encoding technique is presented that allows efficient frequency band allocation based on the number of required instances of time and date variables. A figure of merit (FOM) relating coding capacity and tag dimensions coined as code density is also introduced. A systematic design process followed by simulations and verified through measurements reveal a high code density of 3.56 bits/cm2 for the presented chipless tag.

  18. A Secure RFID Tag Authentication Protocol with Privacy Preserving in Telecare Medicine Information System.

    Science.gov (United States)

    Li, Chun-Ta; Weng, Chi-Yao; Lee, Cheng-Chi

    2015-08-01

    Radio Frequency Identification (RFID) based solutions are widely used for providing many healthcare applications include patient monitoring, object traceability, drug administration system and telecare medicine information system (TMIS) etc. In order to reduce malpractices and ensure patient privacy, in 2015, Srivastava et al. proposed a hash based RFID tag authentication protocol in TMIS. Their protocol uses lightweight hash operation and synchronized secret value shared between back-end server and tag, which is more secure and efficient than other related RFID authentication protocols. Unfortunately, in this paper, we demonstrate that Srivastava et al.'s tag authentication protocol has a serious security problem in that an adversary may use the stolen/lost reader to connect to the medical back-end server that store information associated with tagged objects and this privacy damage causing the adversary could reveal medical data obtained from stolen/lost readers in a malicious way. Therefore, we propose a secure and efficient RFID tag authentication protocol to overcome security flaws and improve the system efficiency. Compared with Srivastava et al.'s protocol, the proposed protocol not only inherits the advantages of Srivastava et al.'s authentication protocol for TMIS but also provides better security with high system efficiency.

  19. Preparative SDS PAGE as an Alternative to His-Tag Purification of Recombinant Amelogenin.

    Science.gov (United States)

    Gabe, Claire M; Brookes, Steven J; Kirkham, Jennifer

    2017-01-01

    Recombinant protein technology provides an invaluable source of proteins for use in structure-function studies, as immunogens, and in the development of therapeutics. Recombinant proteins are typically engineered with "tags" that allow the protein to be purified from crude host cell extracts using affinity based chromatography techniques. Amelogenin is the principal component of the developing enamel matrix and a frequent focus for biomineralization researchers. Several groups have reported the successful production of recombinant amelogenins but the production of recombinant amelogenin free of any tags, and at single band purity on silver stained SDS PAGE is technically challenging. This is important, as rigorous structure-function research frequently demands a high degree of protein purity and fidelity of protein sequence. Our aim was to generate His-tagged recombinant amelogenin at single band purity on silver stained SDS PAGE for use in functionality studies after His-tag cleavage. An acetic acid extraction technique (previously reported to produce recombinant amelogenin at 95% purity directly from E. coli ) followed by repeated rounds of nickel column affinity chromatography, failed to generate recombinant amelogenin at single band purity. This was because following an initial round of nickel column affinity chromatography, subsequent cleavage of the His-tag was not 100% efficient. A second round of nickel column affinity chromatography, used in attempts to separate the cleaved His-tag free recombinant from uncleaved His-tagged contaminants, was still unsatisfactory as cleaved recombinant amelogenin exhibited significant affinity for the nickel column. To solve this problem, we used preparative SDS PAGE to successfully purify cleaved recombinant amelogenins to single band purity on silver stained SDS PAGE. The resolving power of preparative SDS PAGE was such that His-tag based purification of recombinant amelogenin becomes redundant. We suggest that acetic

  20. Tagging Water Sources in Atmospheric Models

    Science.gov (United States)

    Bosilovich, M.

    2003-01-01

    Tagging of water sources in atmospheric models allows for quantitative diagnostics of how water is transported from its source region to its sink region. In this presentation, we review how this methodology is applied to global atmospheric models. We will present several applications of the methodology. In one example, the regional sources of water for the North American Monsoon system are evaluated by tagging the surface evaporation. In another example, the tagged water is used to quantify the global water cycling rate and residence time. We will also discuss the need for more research and the importance of these diagnostics in water cycle studies.