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Sample records for strong antioxidant activity

  1. Antioxidant and cytotoxic activities of carob tree fruit pulps are strongly influenced by gender and cultivar.

    Science.gov (United States)

    Custodio, L; Fernandes, E; Escapa, A L; Fajardo, A; Aligue, R; Albericio, F; Neng, N R; Nogueira, J M F; Romano, A

    2011-07-13

    Extracts from fruit pulps of six female cultivars and two hermaphrodite Portuguese carob trees [(Ceratonia siliqua L., Fabaceae)] exhibited strong antioxidant activity and were rich in phenolic compounds. The extracts decreased the viability of different human cancer cell lines on a dose- and time-dependent manner. Gender and cultivar significantly influenced the chemical content and the biological activities of the extracts. Extracts from hermaphrodite trees had a higher content of phenolic compounds, and exhibited higher antioxidant and cytotoxic activities. Among females, cv. Aida had the highest radical scavenging activity and total content of phenolics, Mulata the highest capacity to inhibit lipid oxidation and Gasparinha the strongest cytotoxic activity on HeLa cells. The decrease in cell viability was associated with apoptosis on HeLa and MDA-MB-231 lines. (+)-Catechin and gallic acid (GA) were the main compounds identified in the extracts, and GA contributed to the antioxidant activity. Our results show that the antioxidant and cytotoxic activities of carob tree fruit pulps are strongly influenced by gender and cultivar, and provide new knowledge about the advantages of hermaphrodite trees over female cultivars, namely, as a source of compounds with biological interest, which may represent an increase of their agronomic interest.

  2. Yucca gloriosa: a source of phenolic derivatives with strong antioxidant activity.

    Science.gov (United States)

    Bassarello, Carla; Bifulco, Giuseppe; Montoro, Paola; Skhirtladze, Alexandre; Benidze, Mariam; Kemertelidze, Ether; Pizza, Cosimo; Piacente, Sonia

    2007-08-08

    On the basis of the biological activities exhibited by the phenolic constituents of Yucca schidigera, the antioxidant activity of the methanol extract of Yucca gloriosa roots was evaluated in the TEAC assay. The strong activity exerted by this extract prompted investigation of its phenolic constituents, yielding three new phenolic derivatives, gloriosaols C, D, and E, along with gloriosaols A and B previously isolated from Y. gloriosa roots and yuccaols C-E isolated from Y. schidigera. ESIMS and NMR data of gloriosaols C-E closely resembled those reported for gloriosaols A and B, two diasteroisomers characterized by unusual spirostructures. Careful inspection of ROESY spectra revealed that gloriosaols C-E are diastereoisomers of gloriosaols A and B. A possible assignment of the relative configuration of gloriosaols C-E, derived according to an integrated NMR-quantum mechanical (QM) approach, which was already applied to the determination of the stereostructures of gloriosaols A and B, is also proposed. Gloriosaols A-E exhibited potent antioxidant activity measured by the TEAC assay, showing the potential use of Y. gloriosa as a source of antioxidant principles.

  3. Strong enhancement of antioxidant activity of Aloe vera extracts by gamma irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Eun Mi; Bai, Hyoung Woo; Lee, Seung Sik; Hong, Sung Hyun [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Cho, Jae Young; Chung, Byung Yeoup [Chonbuk National University, Jeonju (Korea, Republic of)

    2011-10-15

    The World Health Organization (WHO) has estimated that approximately 80% of individuals rely on traditional medicines. Among over 400 Aloe species, Aloe vera was the most accepted species for various medical, cosmetic and neutraceutical purposes. Aloe vera (syn.: Aloe barbadensis Miller) was a perennial succulent plant belonging to the Aloeaceae family (subfamily of the Asphodelaceae). It has been reported that Aloe vera extracts were useful in the treatment of wound and burn healing, minor skin infections, sebaceous cyst, diabetes, and elevated blood lipids in humans. Recent studies have shown that treatment with either Aloe vera crude gel or its extracts, such as acemannan, {beta}-sitosterol, and others, resulted in faster healing of wounds by stimulating fibroblast proliferation, collagen deposition, angiogenesis, and production of growth factors. Ionizing radiation technology has been developed to improve our daily life such as cancer therapy and sterilizing tool due to its unique feature that could be penetrated biomaterials leading to alter their own physical properties. More recently, many studies have attempted to apply the radiation technology to enhance their biological activities. At present, however, very little was known about whether naturally-occurring phenolic compounds of ethanolic aloe gel extracts that were altered their biological activities by ionizing radiation to serve as antioxidant in the body to reduce ROS produced by the stresses. The purpose of the current study was to investigate the influence of gamma irradiation on antioxidant activity of Aloe vera extracts, and open insight new possibilities that gamma ray could be a powerful tool for improving its own biological activities

  4. Strong enhancement of antioxidant activity of Aloe vera extracts by gamma irradiation

    International Nuclear Information System (INIS)

    Lee, Eun Mi; Bai, Hyoung Woo; Lee, Seung Sik; Hong, Sung Hyun; Cho, Jae Young; Chung, Byung Yeoup

    2011-01-01

    The World Health Organization (WHO) has estimated that approximately 80% of individuals rely on traditional medicines. Among over 400 Aloe species, Aloe vera was the most accepted species for various medical, cosmetic and neutraceutical purposes. Aloe vera (syn.: Aloe barbadensis Miller) was a perennial succulent plant belonging to the Aloeaceae family (subfamily of the Asphodelaceae). It has been reported that Aloe vera extracts were useful in the treatment of wound and burn healing, minor skin infections, sebaceous cyst, diabetes, and elevated blood lipids in humans. Recent studies have shown that treatment with either Aloe vera crude gel or its extracts, such as acemannan, β-sitosterol, and others, resulted in faster healing of wounds by stimulating fibroblast proliferation, collagen deposition, angiogenesis, and production of growth factors. Ionizing radiation technology has been developed to improve our daily life such as cancer therapy and sterilizing tool due to its unique feature that could be penetrated biomaterials leading to alter their own physical properties. More recently, many studies have attempted to apply the radiation technology to enhance their biological activities. At present, however, very little was known about whether naturally-occurring phenolic compounds of ethanolic aloe gel extracts that were altered their biological activities by ionizing radiation to serve as antioxidant in the body to reduce ROS produced by the stresses. The purpose of the current study was to investigate the influence of gamma irradiation on antioxidant activity of Aloe vera extracts, and open insight new possibilities that gamma ray could be a powerful tool for improving its own biological activities

  5. Antioxidants and embryo phenotype: is there experimental evidence for strong integration of the antioxidant system?

    Science.gov (United States)

    Possenti, Cristina Daniela; Karadas, Filiz; Colombo, Graziano; Caprioli, Manuela; Rubolini, Diego; Milzani, Aldo; Donne, Isabella Dalle; Saino, Nicola; Parolini, Marco

    2017-02-15

    Organisms have evolved complex defense systems against oxidative stress. Bird eggs contain maternally derived antioxidants that protect embryos from oxidative damage. The antioxidant system components are thought to be integrated, but few studies have analyzed the covariation between antioxidant concentrations, embryo 'oxidative status' and morphology. In addition, no study has tested the effects of experimental change in yolk antioxidant concentration on other antioxidants, on their reciprocal relationships and on their relationships with embryo oxidative status or growth, which are expected if antioxidants defenses are integrated. In yellow-legged gull ( Larus michahellis ) embryos, we analyzed the covariation between several antioxidants, markers of 'oxidative status' [total antioxidant capacity (TAC), concentration of pro-oxidants (TOS), lipid peroxidation (LPO) and protein carbonylation (PC)] in the yolk, liver and brain, and morphology. Yolk and liver antioxidant concentrations were positively correlated reciprocally and with embryo size, and positively predicted TAC but not oxidative status. TOS and LPO were positively correlated in the liver, while TAC and LPO were negatively correlated in the brain. Weak relationships existed between antioxidants and TOS, PC and LPO. The effects of antioxidants on oxidative status and morphology were non-synergistic. An experimental physiological increase in yolk vitamin E had very weak effects on the relationships between other antioxidants or oxidative status and vitamin E concentration, the concentration of other antioxidants or oxidative status; the covariation between other antioxidants and oxidative status, and relationships between morphology or oxidative status and other antioxidants, challenging the common wisdom of strong functional relationships among antioxidants, at least for embryos in the wild. © 2017. Published by The Company of Biologists Ltd.

  6. High content of dopamine, a strong antioxidant, in Cavendish banana.

    Science.gov (United States)

    Kanazawa, K; Sakakibara, H

    2000-03-01

    A strong water-soluble antioxidant was identified in the popular commercial banana Musa cavendishii. It is dopamine, one of the catecholamines. For suppressing the oxygen uptake of linoleic acid in an emulsion and scavenging a diphenylpicrylhydrazyl radical, dopamine had greater antioxidative potency than glutathione, food additives such as butylated hydroxyanisole and hydroxytoluene, flavone luteolin, flavonol quercetin, and catechin, and similar potency to the strongest antioxidants gallocatechin gallate and ascorbic acid. Banana contained dopamine at high levels in both the peel and pulp. Dopamine levels ranged from 80-560 mg per 100 g in peel and 2.5-10 mg in pulp, even in ripened bananas ready to eat. Banana is thus one of the antioxidative foods.

  7. A novel antioxidant peptide, purified from Bacillus amyloliquefaciens, showed strong antioxidant potential via Nrf-2 mediated heme oxygenase-1 expression.

    Science.gov (United States)

    Rahman, Md Saifur; Hee Choi, Yun; Seok Choi, Yoon; Alam, Md Badrul; Han Lee, Sang; Cheol Yoo, Jin

    2018-01-15

    A novel antioxidant peptide YD1 (∼1.0kDa), purified from the strain Bacillus amyloliquefaciens CBSYD1, displayed activity in several in vitro assays and was also efficient against Gram-positive, Gram-negative as well as multidrug-resistant (MDR) bacteria. Strain growth was adapted to bile-salt conditions where a clear halos-zone was observed in a bile-salt plate assay and was viable in different digestive track conditions. YD1 treatment on RAW 264.7 cells increased the transcriptional and translational activities of NF-E2-related factor-2 (Nrf-2) through the enhanced levels of heme oxygenase-1 (HO-1). Furthermore, the YD1-treated group showed higher levels of antioxidant enzymes compared to the oxidative stress group. YD1 demonstrated a strong antioxidant activity by decreasing nitric oxide (NO) and reactive oxygen species (ROS) in RAW 264.7 cells. This study suggests that YD1 and the strain could be a natural antioxidant and a probiotic candidate respectively. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Antioxidant activity of puha (Sonchus oleraceus L.) as assessed by the cellular antioxidant activity (CAA) assay.

    Science.gov (United States)

    McDowell, Arlene; Thompson, Scott; Stark, Mirjam; Ou, Zong-Quan; Gould, Kevin S

    2011-12-01

    There is considerable interest in antioxidant dietary components that can be protective against degenerative diseases in humans. Puha (Sonchus oleraceus L.) is a rich source of polyphenols, and exhibits strong antioxidant activity as measured by the 2,2-diphenylpicrylhydrazyl (DPPH) assay. However, the potential of puha to protect against degenerative diseases requires that low molecular weight antioxidants (LMWA) are absorbed by, and active in, human cells. The cellular antioxidant activity (CAA) assay was used to investigate the antioxidant activity of puha leaf extracts. Preparation methods of freezing and freeze-drying reduced the total polyphenolic content compared with fresh puha, but did not affect the LMWA potential as determined by the DPPH assay. The IC(50) values were 0.012 ± 0.003 mg/mL and 0.010 ± 0.005 mg/mL for freeze-dried and fresh puha leaves, respectively. Using the CAA assay, it was shown that LMWAs from foliar extracts of puha were effectively absorbed into HepG2 cells, and exerted antioxidant activity at levels comparable to those of extracts from blueberry fruits, the much-touted antioxidant superfood. Methylene blue staining of HepG2 cells indicated that puha extracts were not cytotoxic at concentrations below 100 mg DW/mL. The data indicate the potential of puha as a nutraceutical supplement for human health. Copyright © 2011 John Wiley & Sons, Ltd.

  9. Antioxidant activity of Potentilla fruticosa

    NARCIS (Netherlands)

    Miliauskas, G.; Beek, van T.A.; Venskutonis, P.R.; Linssen, J.P.H.; Waard, de P.; Sudhölter, E.J.R.

    2004-01-01

    The molecular structures of the radical scavenging compounds present in extracts of Potentilla fruticosa blossoms were elucidated and the antioxidant activities of various extracts were determined. The activities of the different fractions were monitored by off-line and on-line RP-HPLC DPPH. and

  10. Antioxidative activity of Geranium macrorrhizum

    NARCIS (Netherlands)

    Miliauskas, G.; Beek, van T.A.; Venskutonis, P.R.; Linssen, J.P.H.; Waard, de P.

    2004-01-01

    The composition of radical-scavenging compounds from Geranium macrorrhizum leaves was analyzed and the antioxidative activities of various extracts was determined. Seven compounds, namely gallic acid, ellagic acid, 4-galloyl quinic acid, the flavonoid quercetin and three of its glycosides,

  11. for antimicrobial and antioxidant activities

    African Journals Online (AJOL)

    Yomi

    2012-01-31

    Jan 31, 2012 ... An acetone extract of Loxostylis alata was separated into six fractions based on polarity by a solvent- solvent fractionation procedure and the different fractions were screened for antimicrobial and antioxidant activities. The number of antimicrobial compounds in the carbon tetrachloride (CCl4), chloroform ...

  12. Antioxidant and antimicrobial activities of polyphenols from ...

    African Journals Online (AJOL)

    the medicinal plants were screened for their antioxidant and antimicrobial activities against pathogenic micro organisms (Staphylococcus aureus, Streptococcus pyogenes, Esherichia coli and Candida albicans). The medicinal plants displayed different polyphenols contents and antioxidant activities. In addition, varying ...

  13. Antioxidant and antibacterial activities of polyphenols from ...

    African Journals Online (AJOL)

    Polyphenols from four medicinal plants of Burkina Faso, Combretum micranthum, Khaya senegalensis, Pterocarpus erinaceus and Sida acuta, were screened for their antioxidant and antimicrobial activities against pathogenic bacteria. The medicinal plants displayed different polyphenols contents and antioxidant activities.

  14. Antioxidant and antimicrobial activities of polyphenols from ...

    African Journals Online (AJOL)

    USER

    2010-05-17

    May 17, 2010 ... The antioxidant properties and antimicrobial potential of three ethnomedicinal plants, (Momordica charanta, Senna alata and Nauclea lafifolia) extracted with acetone were investigated. Polyphenols from the medicinal plants were screened for their antioxidant and antimicrobial activities against pathogenic.

  15. Cosmeceutical values, antimicrobial activities and antioxidant ...

    African Journals Online (AJOL)

    Jane

    2011-10-24

    Oct 24, 2011 ... Key words: Antimicrobial, antioxidant, ethanol extract, water extract, cashew leaves, cosmeceutical. INTRODUCTION ... Anacardium occidentale (Cashew) leaves extract could ..... Antioxidant activity at different contact time for treatment of different concentration CLE in water with different amount of GAC.

  16. Antioxidant and Anti-Inflammatory Activities of Barettin

    OpenAIRE

    Lind, Karianne F.; Hansen, Espen; ?sterud, Bjarne; Eilertsen, Karl-Erik; Bayer, Annette; Engqvist, Magnus; Leszczak, Kinga; J?rgensen, Trond ?.; Andersen, Jeanette H.

    2013-01-01

    In this paper, we present novel bioactivity for barettin isolated from the marine sponge Geodia barretti. We found that barettin showed strong antioxidant activity in biochemical assays as well as in a lipid peroxidation cell assay. A de-brominated synthetic analogue of barettin did not show the same activity in the antioxidant cell assay, indicating that bromine is important for cellular activity. Barettin was also able to inhibit the secretion of the inflammatory cytokines IL-1β and TNFα fr...

  17. Comparison of Antioxidant Evaluation Assays for Investigating Antioxidative Activity of Gallic Acid and Its Alkyl Esters in Different Food Matrices.

    Science.gov (United States)

    Phonsatta, Natthaporn; Deetae, Pawinee; Luangpituksa, Pairoj; Grajeda-Iglesias, Claudia; Figueroa-Espinoza, Maria Cruz; Le Comte, Jérôme; Villeneuve, Pierre; Decker, Eric A; Visessanguan, Wonnop; Panya, Atikorn

    2017-08-30

    The addition of antioxidants is one of the strategies to inhibit lipid oxidation, a major cause of lipid deterioration in foods leading to rancidity development and nutritional losses. However, several studies have been reported that conventional antioxidant assays, e.g., TPC, ABTS, FRAP, and ORAC could not predict antioxidant performance in several foods. This study aimed to investigate the performance of two recently developed assays, e.g., the conjugated autoxidizable triene (CAT) and the apolar radical-initiated conjugated autoxidizable triene (ApoCAT) assays to predict the antioxidant effectiveness of gallic acid and its esters in selected food models in comparison with the conventional antioxidant assays. The results indicated that the polarities of the antioxidants have a strong impact on antioxidant activities. In addition, different oxidant locations demonstrated by the CAT and ApoCAT assays influenced the overall antioxidant performances of the antioxidants with different polarities. To validate the predictability of the assays, the antioxidative performance of gallic acid and its alkyl esters was investigated in oil-in-water (O/W) emulsions, bulk soybean oils, and roasted peanuts as the lipid food models. The results showed that only the ApoCAT assay could be able to predict the antioxidative performances in O/W emulsions regardless of the antioxidant polarities. This study demonstrated that the relevance of antioxidant assays to food models was strongly dependent on physical similarities between the tested assays and the food structure matrices.

  18. Studies of the structure-antioxidant activity relationships and antioxidant activity mechanism of iridoid valepotriates and their degradation products.

    Science.gov (United States)

    Wang, Feifei; Zhang, Yumei; Wu, Shouhai; He, Yi; Dai, Zhong; Ma, Shuangcheng; Liu, Bin

    2017-01-01

    Oxidative stress has been associated with diverse diseases, including obesity, cancer and neurodegeneration. In fact, Valeriana jatamansi Jones (valerian) and its extracts possess strong antioxidant activities that extend their application in clinical practice to the treatment of these illnesses, even though the underlying mechanisms are not well understood. Iridoid valepotriate, a characteristic iridoid ester in valerian with poor chemical stability, possesses considerable antioxidant components. The original compounds and their degradation products have been found to exhibit strong antioxidant activities. However, the relationship between their structure and antioxidant effects and the mechanism underlying their oxidation resistance remain unclear. A forced degradation study using three iridoid valepotriates (valtrate, acevaltrate and 1-β acevaltrate) was performed in this work, and the structures of their degradation products were estimated by TLC-MS and LC-MS. Comparison of the antioxidant activities of the iridoid valepotriates before and after forced degradation revealed that degradation reduced the activities of the iridoid valepotriates in free radical scavenging and cytotoxic and cell apoptosis tests. The results suggested that the oxirane nucleus is important for defining the antioxidant profile of iridoid valepotriate. We uncovered possible mechanisms that could explain the antioxidant activities, including the generation of two hydroxyl groups through intramolecular transfer of an H• from an oxirane ring and a reduction in ROS levels through interactions with GABAergic signalling pathways.

  19. Studies of the structure-antioxidant activity relationships and antioxidant activity mechanism of iridoid valepotriates and their degradation products

    Science.gov (United States)

    Wang, Feifei; Zhang, Yumei; Wu, Shouhai; He, Yi; Dai, Zhong; Liu, Bin

    2017-01-01

    Oxidative stress has been associated with diverse diseases, including obesity, cancer and neurodegeneration. In fact, Valeriana jatamansi Jones (valerian) and its extracts possess strong antioxidant activities that extend their application in clinical practice to the treatment of these illnesses, even though the underlying mechanisms are not well understood. Iridoid valepotriate, a characteristic iridoid ester in valerian with poor chemical stability, possesses considerable antioxidant components. The original compounds and their degradation products have been found to exhibit strong antioxidant activities. However, the relationship between their structure and antioxidant effects and the mechanism underlying their oxidation resistance remain unclear. A forced degradation study using three iridoid valepotriates (valtrate, acevaltrate and 1-β acevaltrate) was performed in this work, and the structures of their degradation products were estimated by TLC-MS and LC-MS. Comparison of the antioxidant activities of the iridoid valepotriates before and after forced degradation revealed that degradation reduced the activities of the iridoid valepotriates in free radical scavenging and cytotoxic and cell apoptosis tests. The results suggested that the oxirane nucleus is important for defining the antioxidant profile of iridoid valepotriate. We uncovered possible mechanisms that could explain the antioxidant activities, including the generation of two hydroxyl groups through intramolecular transfer of an H• from an oxirane ring and a reduction in ROS levels through interactions with GABAergic signalling pathways. PMID:29232391

  20. Invitro Antioxidant Activities on Leaf Extracts of Syzygium Malaccense (L.) Merr and Perry

    OpenAIRE

    Savitha, Rabeque C; Padmavathy, S; Sundhararajan, A

    2011-01-01

    Oxidative stress and impaired antioxidant system have been implicated in the pathophysiology of various diseases. The objective of the study was to determine the quantitative phytochemicals and invitro antioxidant activity of fresh leaves of Syzygium malaccense. The result showed that the methanolic extract exhibited strong antioxidant activity and contains a higher amount of phenolics and flavonoids when compared to aqueous extract.

  1. Invitro antioxidant activities on leaf extracts of syzygium malaccense (L.) merr and perry.

    Science.gov (United States)

    Savitha, Rabeque C; Padmavathy, S; Sundhararajan, A

    2011-04-01

    Oxidative stress and impaired antioxidant system have been implicated in the pathophysiology of various diseases. The objective of the study was to determine the quantitative phytochemicals and invitro antioxidant activity of fresh leaves of Syzygium malaccense. The result showed that the methanolic extract exhibited strong antioxidant activity and contains a higher amount of phenolics and flavonoids when compared to aqueous extract.

  2. Antioxidant Activity from Various Tomato Processing

    Directory of Open Access Journals (Sweden)

    Retno Sri Iswari

    2016-04-01

    Full Text Available Tomato is one of the high antioxidant potential vegetables. Nowadays, there are many techniques of tomato processings instead of fresh consumption, i.e. boiled, steamed, juiced and sauteed. Every treatment of cooking will influence the chemical compound inside the fruits and the body's nutrition intake. It is important to conduct the research on antioxidant compound especially lycopene, β-carotene, vitamin C, α-tocopherol, and its activity after processing. This research has been done using the experimental method. Tomatoes were cooked into six difference ways, and then it was extracted using the same procedure continued with antioxidant measurement. The research results showed that steaming had promoted the higher antioxidant numbers (lycopene. α-tocopherol, β-carotene and vitamin C and higher TCA and antioxidant activities in the tomatoes than other processings. It was indicated that steaming was the best way to enhance amount, capacity and activities of antioxidants of the tomatoes.

  3. Antidiabetic, Antihyperlipidemic and Antioxidant Activities of ...

    African Journals Online (AJOL)

    Those with blood glucose levels > 190 ± 8 mg/dl were administered the methanol leaf extract of. Buchanania lanzan ... blood glucose level, serum lipid profile, and significantly (p < 0.05) increased antioxidant activity as ... Conclusion: MEBL exhibits antidiabetic, antihyperlipidemic and antioxidant activities in diabetic rat and.

  4. Antioxidant activity of lichen Cetraria aculeata

    Directory of Open Access Journals (Sweden)

    Tomović Jovica

    2016-01-01

    Full Text Available The aim of the present study is to investigate the antioxidant properties of the lichen Cetraria aculeata. Antioxidant activity of the methanol and ethyl acetate extracts of lichen was tested by different methods including determination of total phenolics content, determination of total antioxidant capacity, DPPH free radical scavenging activity, inhibitory activity towards lipid peroxidation, ferrous ion chelating ability and hydroxyl radical scavenging activity. The extracts of the lichen C. aculeata showed significant antioxidant activity. The methanol extract showed higher values for total phenolics and total antioxidant capacity compared to the ethyl acetate extract, while the ethyl acetate extract demonstrated better results for DPPH radical scavenging, inhibitory activity towards lipid peroxidation, chelating ability and hydroxyl radical scavenging than the methanol extract. This is the first report of the antioxidant properties of Cetraria aculeata growing in Serbia. The results of antioxidant activity indicate the application of this lichen as source of natural antioxidants that could be used as a possible food supplement, in the pharmaceutical industry and in the treatment of various diseases.

  5. Antiradical and antioxidant activities of new bio-antioxidants.

    Science.gov (United States)

    Kancheva, V D; Saso, L; Angelova, S E; Foti, M C; Slavova-Kasakova, A; Daquino, C; Enchev, V; Firuzi, O; Nechev, J

    2012-02-01

    Antioxidants could be promising agents for management of oxidative stress-related diseases. New biologically active compounds, belonging to a rare class of natural lignans with antiangiogenic, antitumoral and DNA intercalating properties, have been recently synthesized. These compounds are benzo[kl]xanthene lignans (1,2) and dihydrobenzofuran neolignans (3,4). The radical scavenging and chain-breaking antioxidant activities of compounds 1-4 were studied by applying different methods: radical scavenging activity by DPPH rapid test, chain-breaking antioxidant activity and quantum chemical calculations. All studied compounds were found to be active as DPPH scavengers but reaction time with DPPH and compounds' concentrations influenced deeply the evaluation. The highest values of radical scavenging activity (%RSAmax) and largest rate constants for reaction with DPPH were obtained for compounds 2 and 3. Comparison of %RSAmax with that of standard antioxidants DL-α-tocopherol (TOH), caffeic acid (CA) and butylated hydroxyl toluene (BHT) give the following new order of %RSA max: TOH (61.1%) > CA (58.6%) > 3 (36.3%) > 2 (28.1%) > 4 (6.7%) > 1 (3.6%) = BHT (3.6%). Chain-breaking antioxidant activities of individual compounds (0.1-1.0 mM) and of their equimolar binary mixtures (0.1 mM) with TOH were determined from the kinetic curves of lipid autoxidation at 80 °C. On the basis of a comparable kinetic analysis with standard antioxidants a new order of the antioxidant efficiency (i.e., protection factor, PF) of compounds 1-4 were obtained: 2 (7.2) ≥ TOH (7.0) ≥ CA (6.7) > 1 (3.1) > 3 (2.2) > ferulic acid FA (1.5) > 4 (0.6); and of the antioxidant reactivity (i.e. inhibition degree, ID): 2 (44.0) > TOH (18.7) > CA (9.3) > 1 (8.4) > 3 (2.8) > FA (1.0) > 4 (0.9). The important role of the catecholic structure in these compounds, which is responsible for the high chain-breaking antioxidant activity, is discussed and a reaction

  6. Identification of Major Phenolic Compounds from Nephelium lappaceum L. and Their Antioxidant Activities

    OpenAIRE

    Thitilertdecha, Nont; Teerawutgulrag, Aphiwat; Kilburn, Jeremy D.; Rakariyatham, Nuansri

    2010-01-01

    Nephelium lappaceum is a tropical fruit whose peel possesses antioxidant properties. Experiments on the isolation and identification of the active constituents were conducted, and on their antioxidant activity using a lipid peroxidation inhibition assay. The methanolic extract of N. lappaceum peels exhibited strong antioxidant properties. Sephadex LH-20 chromatography was utilized in the isolation of each constituent and the antioxidant properties of each was studied. The isolated compounds w...

  7. Cellular antioxidant activity of common vegetables.

    Science.gov (United States)

    Song, Wei; Derito, Christopher M; Liu, M Keshu; He, Xiangjiu; Dong, Mei; Liu, Rui Hai

    2010-06-09

    The measurement of antioxidant activity using biologically relevant assays is important to screen fruits, vegetables, natural products, and dietary supplements for potential health benefits. The cellular antioxidant activity (CAA) assay quantifies antioxidant activity using a cell culture model and was developed to meet the need for a more biologically representative method than the popular chemistry antioxidant capacity measures. The objective of the study was to determine the CAA, total phenolic contents, and oxygen radical absorbance capacity (ORAC) values of 27 vegetables commonly consumed in the United States. Beets, broccoli, and red pepper had the highest CAA values, whereas cucumber had the lowest. CAA values were significantly correlated to total phenolic content. Potatoes were found to be the largest contributors of vegetable phenolics and CAA to the American diet. Increased fruit and vegetable consumption is an effective strategy to increase antioxidant intake and decrease oxidative stress and may lead to reduced risk of developing chronic diseases, such as cancer and cardiovascular disease.

  8. Phytochemical Screening and Antioxidant Activities of Some ...

    African Journals Online (AJOL)

    Purpose: Oxidative stress has been shown to play an important role in the development of anaemia in malaria. Indeed, increase in total antioxidant status has been shown to be important in recovery from malaria. The antioxidant activities of four medicinal plants traditionally used in the treatment of malaria in southwestern ...

  9. Potent antioxidant dendrimers lacking pro-oxidant activity.

    Science.gov (United States)

    Lee, Choon Young; Sharma, Ajit; Uzarski, Rebecca L; Cheong, Jae Eun; Xu, Hao; Held, Rich A; Upadhaya, Samik K; Nelson, Julie L

    2011-04-15

    It is well known that antioxidants have protective effects against oxidative stress. Unfortunately, in the presence of transition metals, antioxidants, including polyphenols with potent antioxidant activities, may also exhibit pro-oxidant effects, which may irreversibly damage DNA. Therefore, antioxidants with strong free radical-scavenging abilities and devoid of pro-oxidant effects would be of immense biological importance. We report two antioxidant dendrimers with a surface rich in multiple phenolic hydroxyl groups, benzylic hydrogens, and electron-donating ring substituents that contribute to their potent free radical-quenching properties. To minimize their pro-oxidant effects, the dendrimers were designed with a metal-chelating tris(2-aminoethyl)amine (TREN) core. The dendritic antioxidants were prepared by attachment of six syringaldehyde or vanillin molecules to TREN by reductive amination. They exhibited potent radical-scavenging properties: 5 times stronger than quercetin and 15 times more potent than Trolox according to the 1,1-diphenyl-2-picrylhydrazyl assay. The antioxidant dendrimers also protected low-density lipoprotein, lysozyme, and DNA against 2,2'-azobis(2-amidinopropane) dihydrochloride-induced free radical damage. More importantly, unlike quercetin and Trolox, the two TREN antioxidant dendrimers did not damage DNA via their pro-oxidant effects when incubated with physiological amounts of copper ions. The dendrimers also showed no cytotoxicity toward Chinese hamster ovary cells. Copyright © 2010 Elsevier Inc. All rights reserved.

  10. Antioxidant and antimicrobial activities of Shorea kunstleri

    Directory of Open Access Journals (Sweden)

    Siti Suria Daud

    2014-08-01

    Full Text Available Objective: To evaluate antioxidant and antimicrobial activities of stembark of Shorea kunstleri (S. kunstleri together with analysis of phytochemical and total phenolic contents. Methods: Extraction was conducted with different solvent polarity of n-hexane, dichloromethane (DCM and methanol by using Soxhlet extraction. Total phenolic content was determined using Folin-Ciocalteu method. Free radical scavenging activity and inhibition of lipid peroxidation were evaluated with DPPH radical scavenging and ferric thiocyanate assays, respectively. Antimicrobial activities were performed using disc diffusion method, minimum inhibition concentration (MIC, minimum bactericidal concentration (MBC, and minimum fungicidal concentration. Results: S. kunstleri stembark extracts revealed presence of steroids, terpenoids, saponins, flavonoids, and phenolic compounds. Methanol extract exhibited the highest total phenolic content and free radical scavenging activity resulting in phenolic content of (8.340±0.003 g GAE/100 g of extract and (95.90±1.07% DPPH inhibition (IC50 value of 18.6 µg/mL, respectively. Ferric thiocyanate assay of n-hexane, DCM, and methanol extracts indicated lipid peroxidation inhibitory activity of (74.20±0.35%, (74.00±0.10%, and (72.80±0.27%, respectively. In antimicrobial and antifungal tests, methanol extract showed inhibition against Staphylococcus aureus (S. aureus, Candida albicans, and Candida tropicalis with inhibition zones of 10-12, 18-22, and 18-19 mm, respectively. The MIC test of methanol extract showed highest inhibition against Candida albicans and S. aureus (0.04 and 0.08 mg/mL, respectively while DCM extract exhibited the highest activity towards Candida tropicalis (MIC value of 0.63 mg/mL. Taken together, MBC test of methanol extract strongly demonstrated bactericidal effect against S. aureus with MBC value of 0.08 mg/mL. Conclusions: The study demonstrated that stembark extracts of S. kunstleri possessed antioxidant

  11. Enteromorpha compressa Exhibits Potent Antioxidant Activity

    Directory of Open Access Journals (Sweden)

    Sanaa M. M. Shanab

    2011-01-01

    Full Text Available The green macroalgae, Enteromorpha compressa (Linnaeus Nees, Ulva lactuca, and E. linza, were seasonally collected from Abu Qir bay at Alexandria (Mediterranean Sea This work aimed to investigate the seasonal environmental conditions, controlling the green algal growth, predominance, or disappearance and determining antioxidant activity. The freshly collected selected alga (E. compressa was subjected to pigment analysis (chlorophyll and carotenoids essential oil and antioxidant enzyme determination (ascorbate oxidase and catalase. The air-dried ground alga was extracted with ethanol (crude extract then sequentially fractionated by organic solvents of increasing polarity (petroleum ether, chloroform, ethyl acetate, and water. Antioxidant activity of all extracts was assayed using different methods (total antioxidant, DPPH [2, 2 diphenyl-1-picrylhydrazyl], ABTS [2, 2 azino-bis ethylbenzthiazoline-6-sulfonic acid], and reducing power, and β-carotene linoleic acid bleaching methods. The results indicated that the antioxidant activity was concentration and time dependent. Ethyl acetate fraction demonstrated higher antioxidant activity against DPPH method (82.80% compared to the synthetic standard butylated hydroxyl toluene (BHT, 88.5%. However, the crude ethanolic extract, pet ether, chloroform fractions recorded lower to moderate antioxidant activities (49.0, 66.0, and 78.0%, resp.. Using chromatographic and spectroscopic analyses, an active compound was separated and identified from the promising ethyl acetate fraction.

  12. Antioxidant activity potential of gamma irradiated carrageenan

    International Nuclear Information System (INIS)

    Abad, Lucille V.; Relleve, Lorna S.; Racadio, Charles Darwin T.; Aranilla, Charito T.; De la Rosa, Alumanda M.

    2013-01-01

    The antioxidant capacity of irradiated κ-, ι-, λ-carrageenans were investigated using the hydroxyl radical scavenging assay, reducing power assay and DPPH radical scavenging capacity assay. The degree of oxidative inhibition increased with increasing concentration and dose. The type of carrageenan had also an influence on its antioxidant activity which followed the order of lambda< iota< kappa. Increase in oxidative property with radiation dose can be attributed mainly to the depolymerization of the carrageenans with corresponding increase in reducing sugar. The antioxidant properties of these carrageenan oligomers were lower than that of ascorbic acid and galactose sugar. - Highlights: • The antioxidant capacity of gamma irradiated κ-, ι-, λ-carrageenans increased with increasing concentration and dose. • The type of carrageenan had an influence on its antioxidant activity which followed the order of lambda< iota< kappa. • Increase in oxidative property with radiation dose can be attributed mainly to the depolymerization of the carrageenans with corresponding increase in reducing sugar

  13. Antioxidant and antimicrobial activities of Canarium schweinfurthii ...

    African Journals Online (AJOL)

    diphenylpicrylhydrazyl (DPPH) radical scavenging assay and the -carotene bleaching test. Butylated hydroxytoluene (BHT) was employed as a positive control. The essential oil showed antioxidant and DPPH radical scavenging activities, and it displayed ...

  14. Phytochemical analysis and antioxidant activities of Combretum ...

    African Journals Online (AJOL)

    Phytochemical analysis and antioxidant activities of Combretum molle and Pericopsis laxiflora. Kossi-Kuma Agbalevon Koevi, Vinsoun Millogo, Jean Baptiste Hzounda Fokou, Abdou Sarr, Georges Anicet Ouedraogo, Emmanuel Bassene ...

  15. Antioxidant activity of the microalga Spirulina maxima

    Directory of Open Access Journals (Sweden)

    M.S. Miranda

    1998-08-01

    Full Text Available Spirulina maxima, which is used as a food additive, is a microalga rich in protein and other essential nutrients. Spirulina contains phenolic acids, tocopherols and ß-carotene which are known to exhibit antioxidant properties. The aim of the present study was to evaluate the antioxidant capacity of a Spirulina extract. The antioxidant activity of a methanolic extract of Spirulina was determined in vitro and in vivo. The in vitro antioxidant capacity was tested on a brain homogenate incubated with and without the extract at 37oC. The IC50 (concentration which causes a 50% reduction of oxidation of the extract in this system was 0.18 mg/ml. The in vivo antioxidant capacity was evaluated in plasma and liver of animals receiving a daily dose of 5 mg for 2 and 7 weeks. Plasma antioxidant capacity was measured in brain homogenate incubated for 1 h at 37oC. The production of oxidized compounds in liver after 2 h of incubation at 37oC was measured in terms of thiobarbituric acid reactant substances (TBARS in control and experimental groups. Upon treatment, the antioxidant capacity of plasma was 71% for the experimental group and 54% for the control group. Data from liver spontaneous peroxidation studies were not significantly different between groups. The amounts of phenolic acids, a-tocopherol and ß-carotene were determined in Spirulina extracts. The results obtained indicate that Spirulina provides some antioxidant protection for both in vitro and in vivo systems.

  16. Antioxidant and Anti-Inflammatory Activities of Barettin

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    Trond Ø. Jørgensen

    2013-07-01

    Full Text Available In this paper, we present novel bioactivity for barettin isolated from the marine sponge Geodia barretti. We found that barettin showed strong antioxidant activity in biochemical assays as well as in a lipid peroxidation cell assay. A de-brominated synthetic analogue of barettin did not show the same activity in the antioxidant cell assay, indicating that bromine is important for cellular activity. Barettin was also able to inhibit the secretion of the inflammatory cytokines IL-1β and TNFα from LPS-stimulated THP-1 cells. This combination of anti-inflammatory and antioxidant activities could indicate that barettin has an atheroprotective effect and may therefore be an interesting product to prevent development of atherosclerosis.

  17. Antioxidant and anti-inflammatory activities of barettin.

    Science.gov (United States)

    Lind, Karianne F; Hansen, Espen; Østerud, Bjarne; Eilertsen, Karl-Erik; Bayer, Annette; Engqvist, Magnus; Leszczak, Kinga; Jørgensen, Trond Ø; Andersen, Jeanette H

    2013-07-22

    In this paper, we present novel bioactivity for barettin isolated from the marine sponge Geodia barretti. We found that barettin showed strong antioxidant activity in biochemical assays as well as in a lipid peroxidation cell assay. A de-brominated synthetic analogue of barettin did not show the same activity in the antioxidant cell assay, indicating that bromine is important for cellular activity. Barettin was also able to inhibit the secretion of the inflammatory cytokines IL-1β and TNFα from LPS-stimulated THP-1 cells. This combination of anti-inflammatory and antioxidant activities could indicate that barettin has an atheroprotective effect and may therefore be an interesting product to prevent development of atherosclerosis.

  18. Antioxidant and antimicrobial activities of native Rosa sp. from British Columbia, Canada.

    Science.gov (United States)

    Yi, Ocksook; Jovel, Eduardo M; Towers, G H Neil; Wahbe, Tanya R; Cho, Dongwuk

    2007-05-01

    Indigenous traditional knowledge and western science have revealed the potential for significant nutritional and therapeutic benefits among natural antioxidants. We investigated antioxidant and antimicrobial activity of rose hip extracts (Rosa nutkana, Rosa pisocarpa and Rosa woodsii) from wild British Columbia populations using liposome oxidation and disc diffusion assays. All extracts exhibited strong antioxidant activity. R. nutkana pericarp extracts contained high phenolic concentrations and showed greater antioxidant and antimicrobial activity than seed extracts. R. woodsii seed extracts had a higher phenolic concentration and greater antioxidant activity than pericarp extracts. Antioxidant activity was correlated with antimicrobial activity, and both extracts showed antimicrobial activity against yeast and Gram-positive bacteria. Our study is the first to demonstrate the antioxidant and antimicrobial properties of wild British Columbia roses. The effectiveness of selected species compared with standards demonstrates the significance of this natural resource to the continued health of human populations, and the need for conservation practices.

  19. Activity-Guided Isolation of Antioxidant Compounds from Rhizophora apiculata

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    Hongbin Xiao

    2012-09-01

    Full Text Available Rhizophora apiculata (R. apiculata contains an abundance of biologically active compounds due its special salt-tolerant living surroundings. In this study, the total phenolic content and antioxidant activities of various extract and fractions of stem of R. apiculata were investigated. Results indicated that butanol fraction possesses the highest total phenolic content (181.84 mg/g GAE/g dry extract with strongest antioxidant abilities. Following in vitro antioxidant activity-guided phytochemical separation procedures, lyoniresinol-3α-O-β-arabinopyranoside (<strong>1strong>, lyoniresinol-3α-O-β-rhamnoside (<strong>2strong>, and afzelechin-3-O-L-rhamno-pyranoside (<strong>3strong> were separated from the butanol fraction. These compounds showed more noticeable antioxidant activity than a BHT standard in the DPPH, ABTS and hydroxyl radical scavenging assays. HPLC analysis results showed that among different plant parts, the highest content of <strong>1strong>–>3strong> was located in the bark (0.068%, 0.066% and 0.011%, respectively. The results imply that the R. apiculata might be a potential source of natural antioxidants and <strong>1strong>–>3strong> are antioxidant ingredients in R. apiculata.

  20. Antioxidant Activity of Hawaiian Marine Algae

    Directory of Open Access Journals (Sweden)

    Anthony D. Wright

    2012-02-01

    Full Text Available Marine algae are known to contain a wide variety of bioactive compounds, many of which have commercial applications in pharmaceutical, medical, cosmetic, nutraceutical, food and agricultural industries. Natural antioxidants, found in many algae, are important bioactive compounds that play an important role against various diseases and ageing processes through protection of cells from oxidative damage. In this respect, relatively little is known about the bioactivity of Hawaiian algae that could be a potential natural source of such antioxidants. The total antioxidant activity of organic extracts of 37 algal samples, comprising of 30 species of Hawaiian algae from 27 different genera was determined. The activity was determined by employing the FRAP (Ferric Reducing Antioxidant Power assays. Of the algae tested, the extract of Turbinaria ornata was found to be the most active. Bioassay-guided fractionation of this extract led to the isolation of a variety of different carotenoids as the active principles. The major bioactive antioxidant compound was identified as the carotenoid fucoxanthin. These results show, for the first time, that numerous Hawaiian algae exhibit significant antioxidant activity, a property that could lead to their application in one of many useful healthcare or related products as well as in chemoprevention of a variety of diseases including cancer.

  1. Antioxidant activity of Hawaiian marine algae.

    Science.gov (United States)

    Kelman, Dovi; Posner, Ellen Kromkowski; McDermid, Karla J; Tabandera, Nicole K; Wright, Patrick R; Wright, Anthony D

    2012-02-01

    Marine algae are known to contain a wide variety of bioactive compounds, many of which have commercial applications in pharmaceutical, medical, cosmetic, nutraceutical, food and agricultural industries. Natural antioxidants, found in many algae, are important bioactive compounds that play an important role against various diseases and ageing processes through protection of cells from oxidative damage. In this respect, relatively little is known about the bioactivity of Hawaiian algae that could be a potential natural source of such antioxidants. The total antioxidant activity of organic extracts of 37 algal samples, comprising of 30 species of Hawaiian algae from 27 different genera was determined. The activity was determined by employing the FRAP (Ferric Reducing Antioxidant Power) assays. Of the algae tested, the extract of Turbinaria ornata was found to be the most active. Bioassay-guided fractionation of this extract led to the isolation of a variety of different carotenoids as the active principles. The major bioactive antioxidant compound was identified as the carotenoid fucoxanthin. These results show, for the first time, that numerous Hawaiian algae exhibit significant antioxidant activity, a property that could lead to their application in one of many useful healthcare or related products as well as in chemoprevention of a variety of diseases including cancer.

  2. In vitro synergistic antioxidant activity and identification of antioxidant components from Astragalus membranaceus and Paeonia lactiflora.

    Directory of Open Access Journals (Sweden)

    Xiaoyan Xu

    Full Text Available Many traditionally used herbs demonstrate significantly better pharmacological effects when used in combination than when used alone. However, the mechanism underlying this synergism is still poorly understood. This study aimed to investigate the synergistic antioxidant activity of Astragalus membranaceus (AME and Paeonia Lactiflora (PL, and identify the potential antioxidant components by 1,1-diphenyl-2-picrylhydrazine (DPPH radical spiking test followed by a high performance liquid chromatography separation combined with diode array detection and tandem mass spectrometry analysis (DPPH-HPLC-DAD-MS/MS. Eight AME-PL combined extracts (E1-E8 were prepared based on bioactivity-guided fractionation. Among them, E1 exhibited the strongest synergistic effect in scavenging DPPH radicals and reducing ferric ions (P<0.05. Moreover, E1 presented strong cytoprotection against H2O2-induced oxidative damage in MRC-5 cells by suppressing the decrease of the superoxide dismutase (SOD, glutathione peroxidase (GSH-Px and catalase (CAT activities. A strong correlation between the increment of total phenolic/flavonoid and synergistic antioxidant activity, especially between the increment of total flavonoid and the increase in ferric reducing power was observed. Finally, seven antioxidant substances were identified in E1 as oxypaeoniflora, catechin, calycosin-7-O-β-D-glucopyranoside, fomononetin-7-O-β-D-glucopyranoside, 9,10-dimethoxy-pterocarpan-3-O-β-D-glucopyranoside, quercetin and 2'-dihydroxy-3',4'-dimethyl-isoflavan-7-O-β-D-glucopyranoside.

  3. Nano-zirconia - Evaluation of its antioxidant and anticancer activity.

    Science.gov (United States)

    Balaji, Siripireddy; Mandal, Badal Kumar; Ranjan, Shivendu; Dasgupta, Nandita; Chidambaram, Ramalingam

    2017-05-01

    Bioactivity of nanomaterials largely depends on its size, shape and crystalline nature. In this work, the smaller sized spherical shaped nano-zirconia (ZrO 2 NPs) (of ~9 to 11nm) was fabricated and studied its biological activity especially antioxidant and cytotoxicity against human colon carcinoma (HCT-116) and human lung carcinoma (A-549) cell lines. To have its real applications in biological aspects readily available Eucalyptus globulus (E. globulus) leaf extract was used as an effective capping and reducing agent for its synthesis. The prepared ZrO 2 NPs was characterized by using different sophisticated instrumentations such as UV-visible spectrophotometer, XRD, FTIR, TEM, SAED, EDX, DLS and fluorescence spectroscopy. Cellular mitochondrial activity i.e. cell viability was measured by MTT assay and anti-oxidant activity was determined by DPPH assay. The smaller sized ZrO 2 NPs showed strong antioxidant activity as well as cytotoxicity on human cancer cell lines. Comparative cytotoxic studies were conducted on human cancerous cell lines using different techniques. Results confirmed the efficient anti-cancer activities of the fabricated ZrO 2 NPs towards the tested cell lines as well as efficient anti-oxidant activity. This is the first study in which E. globulus leaf extract was used to synthesize smaller spherical shaped ZrO 2 NPs for improved bioactivity i.e. antioxidant and cytotoxicity. Copyright © 2017 Elsevier B.V. All rights reserved.

  4. Phenolic Extracts from Acacia mangium Bark and Their Antioxidant Activities

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    Liangliang Zhang

    2010-05-01

    Full Text Available Phenolic compounds are present at very high concentrations in the bark of Acacia mangium. These compounds are known to have strong antioxidant activity and thus different beneficial effects on human health. Phenolic compounds in bark of A. mangium were extracted and their antioxidant activities were investigated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH free radical-scavenging and ferric-reducing antioxidant power (FRAP assays. A central composite design has been employed to optimize the experimental conditions for a high total phenolic content and antioxidant activity. The desirability function approach has been employed to simultaneously optimize the three responses: total phenols, antiradical activity and FRAP. An extraction time of 90 min, liquid-solid ratio of 5, and temperature of 50 °C was predicted for the optimum experimental conditions using the desirability function. A significant linear relationship between antioxidant potency, antiradical activity and the content of phenolic compounds of bark extracts was observed. The structures of condensed tannins isolated from A. mangium were characterized by MALDI-TOF MS analyses. Condensed tannin oligomers from A. mangium were shown to be heterogeneous mixtures consisting of procyanidin and prodelphinidin structural units with polymerization degrees up to 9.

  5. Phenolic extracts from Acacia mangium bark and their antioxidant activities.

    Science.gov (United States)

    Zhang, Liangliang; Chen, Jiahong; Wang, Yongmei; Wu, Dongmei; Xu, Man

    2010-05-14

    Phenolic compounds are present at very high concentrations in the bark of Acacia mangium. These compounds are known to have strong antioxidant activity and thus different beneficial effects on human health. Phenolic compounds in bark of A. mangium were extracted and their antioxidant activities were investigated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging and ferric-reducing antioxidant power (FRAP) assays. A central composite design has been employed to optimize the experimental conditions for a high total phenolic content and antioxidant activity. The desirability function approach has been employed to simultaneously optimize the three responses: total phenols, antiradical activity and FRAP. An extraction time of 90 min, liquid-solid ratio of 5, and temperature of 50 degrees C was predicted for the optimum experimental conditions using the desirability function. A significant linear relationship between antioxidant potency, antiradical activity and the content of phenolic compounds of bark extracts was observed. The structures of condensed tannins isolated from A. mangium were characterized by MALDI-TOF MS analyses. Condensed tannin oligomers from A. mangium were shown to be heterogeneous mixtures consisting of procyanidin and prodelphinidin structural units with polymerization degrees up to 9.

  6. Compositional Analysis and Antioxidant Activity of Volatile ...

    African Journals Online (AJOL)

    camphor and phenolic compounds. There is no significant difference between the radical scavenging activities of the two essential oils. Keywords: S. verticillata, S. suffruticosa, essential oil, antioxidant activity, GC-MS, HPLC activity. Tropical Journal of Pharmaceutical Research is indexed by Science Citation Index ...

  7. Proliferative and antioxidant activity of Symphytum officinale root extract.

    Science.gov (United States)

    Sowa, Ireneusz; Paduch, Roman; Strzemski, Maciej; Zielińska, Sylwia; Rydzik-Strzemska, Ewelina; Sawicki, Jan; Kocjan, Ryszard; Polkowski, Janusz; Matkowski, Adam; Latalski, Michał; Wójciak-Kosior, Magdalena

    2018-03-01

    The root of Symphytum officinale L. is commonly used in folk medicine to promote the wound healing, reduce the inflammation and in the treatment of broken bones. The objective of our investigation was to analyse the extract from S. officinale in term of its antioxidant activity and the effect on cell viability and proliferation of human skin fibroblast (HSF). Moreover, the quantification of main phenolics and allantoin was conducted using HPLC-DAD method. Five compounds were found: rosmarinic, p-hydroxybenzoic, caffeic, chlorogenic and p-coumaric acid. DPPH, FRAP and TPC assay showed the high antioxidant activity of the extract. MTT test proved the stimulatory effect on cell metabolism and viability of HSF cells. Moreover, no changes in cytoskeleton structure and cells shape were observed. The obtained results indicate that non-toxic extract from S. officinale root has strong antioxidant potential and a beneficial effect on human skin fibroblasts.

  8. Preliminary phytochemical screening and antioxidant activity of ...

    African Journals Online (AJOL)

    GAE) and the reducing power of 0.071±0.03nm was obtained. The DPPH scavenging activity of the extract was found to be promising. There is no significant difference (p<0.05) in the antioxidant activity between the extract and those of the ...

  9. Antioxidant activity directed isolations form punica granatum

    International Nuclear Information System (INIS)

    Siddiqi, R.; Saeed, M.G.; Sayeed, S.A.

    2012-01-01

    The extracts derived from pomegranate juice following antioxidant activity directed isolation were screened for their antioxidant activity through their ability to scavenge 2,2- diphenyl-l-picrylhydrazyl (DPPH) radicals. Only fractions which exhibited >50 / 0 DPPH scavenging effect at each step of isolation were selected for further purification and their ability to reduce peroxide formation (peroxide value) in heated com oil. Phytochemical analysis of the pure compounds finally obtained, revealed the presence of pelargonidin-3- galactose (Pg-3-galactose), cyanidin-3-glucose (Cy-3-Glucose), gallic acid, quercetin and myricetin in the fractions exhibiting >50% DPPH scavenging potential. The order of antioxidant activity of these pure compounds by DPPH method was found to be gallic acid> quercetin> myricetin> Cy-3-galactose> Pg-3-Glucose while order with respect to reduction in peroxide value (PV) was the reverse of DPPH. (author)

  10. Antioxidant activities of Eryngium caucasicum inflorescence.

    Science.gov (United States)

    Motallebi Riekandeh, S; Mazandarani, M; Ebrahimzadeh, M A; Zargari, M

    2016-03-01

    Medicinal plants are good sources for discovery of safe and new antioxidants. The aim of present study was to evaluate the efficiencies of three methods for evaluated for antioxidants from Eryngium caucasicum. Ultrasonically assisted extraction, Soxhlet extraction and percolation method were evaluated for the extraction of antioxidants from E. caucasicum inflorescence. Antioxidant activities of extracts were evaluated with four different in vitro tests. Soxhlet extract had higher total phenolic and flavonoid contents than other extracts. Soxhlet extract showed the highest activity in DPPH radical scavenging (IC50 = 83.1 ± 2.1 µg ml-1). Soxhlet extract showed the best activity in iron chelatory (IC50 = 272 ± 6.3 µg ml-1) followed by ultrasonic extract. Percolation extract showed higher NO radical scavenging (IC50 = 390 ± 11.4 µg ml-1) than other extracts. Extracts showed good reducing power (p > 0.05). The results obtained indicated that all three extraction methods especially Soxhlet method could effectively extract antioxidants from this plant.

  11. Antioxidant Enzyme Activities of some Brassica Species

    Directory of Open Access Journals (Sweden)

    Rodica SOARE

    2017-11-01

    Full Text Available This paper set out to comparatively study five species: white cabbage (Brassica oleracea L. var. capitata alba Alef., red cabbage (Brassica oleracea L. var. capitata f. rubra Alef., Kale (Brassica oleracea L. var. Acephala, cauliflower (Brassica oleracea var. botrytis and broccoli (Brassica oleracea var. cymosa in order to identify those with high enzymatic and antioxidant activities. The enzymatic activity of superoxide dismutase (SOD, catalase (CAT and soluble peroxidase (POX as well as the antioxidant activity against 2.2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS radical cation were determined. Total superoxide dismutase activity was measured spectrophotometrically based on inhibition in the photochemical reduction of nitroblue tetrazolium. Total soluble peroxidase was assayed by measuring the increase in A436 due to the guaiacol oxidation and the catalase activity was assayed through the colorimetric method. The capacity of extracts to scavenge the ABTS radical cation was assessed colorimetric using Trolox as a standard. The obtained results show that studied enzymatic activities and the antioxidant activity against ABTS vary depending on the analyzed species. So, among the studied Brassicaceae species, it emphasize red cabbage with the highest enzymatic activity (CAT 22.54 mM H2O2/min/g and POX 187.2 mM ΔA/1min/1g f.w. and kale with highest antioxidant activity, of 767 μmol TE/100g f.w. The results of this study recommendintroducing the studied varieties in diet due to the rich antioxidant properties.

  12. Colour, phenolic content and antioxidant activity of grape juice

    Directory of Open Access Journals (Sweden)

    Vívian Maria Burin

    2010-12-01

    Full Text Available Viticultural practices in the State of Santa Catarina, Brazil, have shown economic growth, with the production of grapes used to produce wines and grape juice. Grapes are rich in phenolic compounds which have drawn attention not only because of their important role in the development of products derived from grapes, but also for their potential beneficial health effects. The objective of this study was to evaluate commercial, organic and homemade grape juices produced in Santa Catarina. Grape juices were analyzed for total phenolic content, colour, and antioxidant activity. The commercial juices had the highest average values for total monomeric anthocyanins and total phenolics. There was a strong positive correlation (R = 0.9566 between the antioxidant activity and total phenolic content for the commercial juice. In addition, the Principle Components Analysis showed a strong positive correlation between the red colour and total monomeric anthocyanins. However, the total monomeric anthocyanis and polymeric anthocyanins showed a negative correlation.

  13. EXTRACTION AND ANTIOXIDANT ACTIVITIES OF TWO SPECIES ...

    African Journals Online (AJOL)

    2013-06-30

    Jun 30, 2013 ... ABSTRACT. The antioxidant of ethanolic extract of two species of Origanum and essential oil of plant. Origanum vulgare were investigated and also the total phenolic and flavonoid content measured. The radical scavenging activity was measured using the 2,2-diphenyl-1- picrylhydrazyl (DPPH) method.

  14. preliminary phytochemical screening and antioxidant activity

    African Journals Online (AJOL)

    DR. AMINU

    ABSTRACT. Antioxidant evaluation of Albizia chevalieri used in Northern Nigerian traditional medicine, was carried out using the free radical scavenging activity of the 1, 1-diphenyl-2-picrylhydrazyl radical. (DPPH), total phenolics content and reducing power assay on the methanol leave extract. The results of the DPPH ...

  15. Hypolipidemic, Hypoglycemic and Antioxidant Activities of Flower ...

    African Journals Online (AJOL)

    HP

    1Department of Chemistry, University of Lucknow, Lucknow-226007, 2Division of Biochemistry, Central Drug. Research ... models, viz, triton WR-1339 - induced hyperlipimea in rats as well as fructose-rich high fat diet. To ..... peroxidation in rat liver microsomes in vitro (Note: Change (%) in antioxidant activity is shown in.

  16. Polyphenols content, antioxidant and antimicrobial activities of ...

    African Journals Online (AJOL)

    25 wild plants were collected from the south of Tunisia. The dried aerial parts were extracted under a continuous reflux set-up in a Soxhlet extractor with hexane, ethyl acetate, methanol and water. The extracts were screened for total phenolic content, antioxidant and antimicrobial activities. Total phenolic contents were ...

  17. Antioxidative Activities of Natural Hydroxy-Bearing ...

    African Journals Online (AJOL)

    ischemia, Alzheimer, Parkinsonism and aging. [1]. Natural antioxidants are capable of trapping radicals to protect .... were analysed using SPSS statistic program. (version 13.0, SPSS Inc, Chicago, IL, USA). RESULTS .... active compound in anti-hemolysis of RBCs among them, so the influence of the concentrations on the ...

  18. CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF APIS ...

    African Journals Online (AJOL)

    CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF APIS. MELLIFERA BEE POLLEN FROM NORTHWEST ALGERIA. A. Rebiai* and T.Lanez. University of El Oued, VTRS Laboratory, P.O. Box 789, 39000, El Oued, Algeria. Received: 08 November 2012 / Accepted: 23 December 2012 / Published online: 31 ...

  19. Hypolipidemic, Hypoglycemic and Antioxidant Activities of Flower ...

    African Journals Online (AJOL)

    Purpose: To investigate the anti-dyslipidemic, anti-oxidant and anti-diabetic activities of the aqueous extract and solvent fractions of A. violacea flowers. Methods: The aqueous extract was fractionated into petroleum ether, ether, chloroform, chloroformmethanol (4:1) and chloroform-methanol (3:2) fractions. Lipid lowering ...

  20. Assessment of antioxidant activity of natural extracts

    Directory of Open Access Journals (Sweden)

    Michel Prost

    2010-01-01

    Full Text Available The aim of this study was investigate and compare the antioxidant activity of extracts from Labiatae and Oleaceae cell cultures and a natural Verbenaceae extract on pig whole blood. The antioxidant activitiy was assessed using the KRL biological test. Extract obtained from Ajuga reptans (Labiatae cell cultures and titrated at 50% of phenylpropanoids expressed as teupolioside (T, extract obtained from Siringa vulgaris (Oleaceae cell cultures and titrated at 50% of phenylpropanoids expressed as verbascoside (V, V and T 50/50 mixture and a natural Verbenaceae extract (NE titrated at 5% of phenylpropanoids, expressed as verbascoside, were tested for their ability to protects pig red blood cells from 2,2’azobis (2-amidinopropane dihydrochloride (AAPH induced hemolysis. The protective effect of the compounds was dose-dependent at concentrations of 0.1 to 1g/L. The active principle in the NE shows an antioxidant activity 2.27 times higher (P<0.01 than the cell culture extract V and 5.11 times higher (P<0.01 than the cell culture extract T. Taking into account the total phenylpropanoids content of cell cultures and natural extract, these results clearly indicate a greater antioxidant activity for the natural Verbaneceae extract.

  1. Carotenoids from Phaffia rhodozyma : Antioxidant activity and ...

    African Journals Online (AJOL)

    The main goal of this work was to establish the stability and antioxidant activity of the extracts obtained through different techniques for recovering carotenoids from Phaffia rhodozyma NRRL-Y 17268. The best conditions for extracting carotenoids through cell rupture with dimethylsulfoxide (DMSO) were found to be a ...

  2. Antioxidant activities of curcumin in allergic rhinitis.

    Science.gov (United States)

    Altıntoprak, Niyazi; Kar, Murat; Acar, Mustafa; Berkoz, Mehmet; Muluk, Nuray Bayar; Cingi, Cemal

    2016-11-01

    We investigated the antioxidant effects of curcumin in an experimental rat model of allergic rhinitis (AR). Female Wistar albino rats (n = 34) were divided randomly into four groups: healthy rats (control group, n = 8), AR with no treatment (AR + NoTr group, n = 10), AR with azelastine HCl treatment (AR + Aze group, n = 8), and AR with curcumin treatment (AR + Curc group, n = 8). On day 28, total blood IgE levels were measured. For measurement of antioxidant activity, the glutathione (GSH) level and catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities were measured in both inferior turbinate tissue and serum. Malondialdehyde (MDA) levels were measured only in inferior turbinate tissue, and paraoxonase (PON) and arylesterase (ARE) activities were measured only in serum. Statistically significant differences were found for all antioxidant measurements (GSH levels and CAT, SOD, GSH-Px activities in the serum and tissue, MDA levels in the tissue, and PON and ARE activities in the serum) between the four groups. In the curcumin group, serum SOD, ARE, and PON and tissue GSH values were higher than the control group. Moreover, tissue GSH levels and serum GSH-Px activities in the curcumin group were higher than in the AR + NoTr group. In the azelastine group, except MDA, antioxidant measurement values were lower than in the other groups. Curcumin may help to increase antioxidant enzymes and decrease oxidative stress in allergic rhinitis. We recommend curcumin to decrease oxidative stress in allergic rhinitis.

  3. Antioxidant enzymes activities in obese Tunisian children

    Directory of Open Access Journals (Sweden)

    Sfar Sonia

    2013-01-01

    Full Text Available Abstract Background The oxidant stress, expected to increase in obese adults, has an important role in the pathogenesis of many diseases. It results when free radical formation is greatly increased or protective antioxidant mechanisms are compromised. The main objective of this study is to evaluate the antioxidant response to obesity-related stress in healthy children. Methods A hundred and six healthy children (54 obese and 52 controls, aged 6–12 years old, participated in this study. The collected data included anthropometric measures, blood pressure, fasting glucose, total cholesterol, triglycerides and enzymatic antioxidants (Superoxide dismutase: SOD, Catalase: CAT and Glutathione peroxidase: GPx. Results The first step antioxidant response, estimated by the SOD activity, was significantly higher in obese children compared with normal-weight controls (p  Conclusions The obesity-related increase of the oxidant stress can be observed even in the childhood period. In addition to the complications of an increased BMI, obesity itself can be considered as an independent risk factor of free radical production resulting in an increased antioxidant response.

  4. Antioxidant activity of Sphaerococcus coronopifolius associated bacteria

    Directory of Open Access Journals (Sweden)

    Nádia Fino

    2014-06-01

    Full Text Available Associated bacteria living on macroalgae surfaces are an interesting source of new secondary metabolites with biological activities. The aim of this study was the isolation and identification of epiphytic bacteria from the marine algae Sphaerococcus coronopifolius and the evaluation of the antioxidant activity of the bacteria extracts. The identification of epiphytic bacteria was determined by 16S rRNA gene sequencing. Bacteria extracts were obtained with methanol and dichloromethane (1:1 extraction. Antioxidant activity was evaluated by quantification of total phenolic content (TPC, 2,2-diphenyl-1-picrylhydrazyl (DPPH radical scavenging activity and oxygen radical absorbent capacity (ORAC. The extracts with higher antioxidant activity were tested on MCF-7 and HepG-2 cell lines in oxidative stress conditions induced by H2O2 at 0.2 mM and 0.5 mM, respectively. In total were isolated 21 Sphaerococcus coronopifolius associated bacteria and identified as Vibrio sp. (28.57%, Shewanella sp. (23.81%, Pseudoalteromonas sp. (19.05%, Bacillus sp. (9.52% and Halomonas sp. (9.52%. Two (9.52% of them presented less than 90% Basic Local Alignment Search Tool (BLAST match. The epiphytic bacteria with the most antioxidant potential evaluated by ORAC and DPPH methods were Sp2, Sp12, Sp23, Sp25 and Sp27. The strain Sp4 show high antioxidant activity in all antioxidant methods (ORAC, DPPH and TPC. In oxidative stress conditions on MCF-7 cell line, the extracts of bacteria (1mg.ml-1: 24hours Sp4 (16.15%, Sp25 (17.95% and Sp27 (10.65% prevented the cell death induced by H2O2. In the HepG-2 cell line was the extracts of Sp2 (9.01%, Sp4 (11.21%, Sp12 (7.20% and Sp23 (8.81% bacteria that high prevented the oxidative stress condition induced by H2O2. In conclusion, the Sphaerococcus coronopifolius associated bacteria can be an interesting and excellent source of marine natural compounds with antioxidant activity.

  5. Antioxidant activity of wheat and buckwheat flours

    Directory of Open Access Journals (Sweden)

    Sedej Ivana J.

    2010-01-01

    Full Text Available Antioxidative activities of wheat flours (type 500 and wholegrain and buckwheat flours (light and wholegrain were tested using 1,1-diphenyl-2-picrylhydrazyl (DPPH·-scavenging activity, reducing power and chelating activity on Fe2+. Also, the content of the total phenolics of ethanolic extracts was estimated. Polyphenolics content (expressed as gallic acid equivalent, GAE in wheat flours varied between 37.1 and 137.2 μg GAE/g extract, while its content in buckwheat flour were at least four time higher and ranged between 476.3 and 618.9μg GAE/g extract. Ethanolic extracts of buckwheat flours exhibited higher antioxidant activities in all the assays, except for chelating activity. Regarding all the obtained results, it can be concluded that bakery products produced with buckwheat flour could be regarded as potential functional foods.

  6. Antioxidant Activities of Total Pigment Extract from Blackberries

    Directory of Open Access Journals (Sweden)

    Jiechao Liu

    2005-01-01

    Full Text Available Total pigment has been extracted from blackberries and its antioxidant activity against lipid peroxidation and scavenging capacities towards superoxide anion radicals, hydroxyl radicals and nitrite in different in vitro systems have been investigated. The total pigment extract from blackberries (TPEB exhibited strong antioxidant activity against lipid peroxidation in a linoleic acid model system and scavenging capacities towards superoxide anion radicals, generated by a pyrogallol autoxidation system or by an illuminating riboflavin system, hydroxyl radicals generated by Fenton reaction, and nitrite. Furthermore, the antioxidant activities were correlated with the concentrations of the TPEB. In the test concentration range, the maximum inhibition percentage against linoleic acid peroxidation was 98.32 % after one week’s incubation, and the maximum scavenging percentages for the free radicals and nitrite inhibition in the above reactive systems reached 90.48, 96.48, 93.58 and 98.94 %, respectively. The TPEB is a natural, edible colorant with excellent antioxidant activities and health benefits and it seems to be applicable in both healthy food and medicine.

  7. In vitro and in vivo antioxidant activities of inulin

    OpenAIRE

    Shang, Hong-Mei; Zhou, Hai-Zhu; Yang, Jun-Yan; Li, Ran; Song, Hui; Wu, Hong-Xin

    2018-01-01

    This study was designed to investigate the in vitro and in vivo antioxidant activities of inulin. The in vitro assays demonstrated that the antioxidant activities of inulin, including the DPPH radical scavenging activity, ABTS scavenging activity and ferric reducing power, were weak and significantly lower than those of Vitamin C (P < 0.05). The influence of dietary supplementation with inulin on the antioxidant status of laying hens was evaluated with in vivo antioxidant assays. The results ...

  8. Evaluation of antioxidant activity and polyphenolic contents of two ...

    African Journals Online (AJOL)

    Total phenolic content were determined by Folin-Ciocalteu test, and antioxidant activity measured using cyclic voltammetry. Correlations between antioxidant activity and total phenolic content were also examined. Within each cultivar, the total phenolic content and antioxidant activity were highest in the peels, followed by ...

  9. Antioxidant, Antitubercular and Cytotoxic Activities of Piper imperiale

    Directory of Open Access Journals (Sweden)

    Sanjib Bhakta

    2012-04-01

    Full Text Available Phenolic compounds are widely distributed in Nature and act as pharmacologically active constituents in many herbal medicines. They have multiple biological properties, most notably antioxidant, antibacterial and cytotoxic activities. In the present study an attempt to correlate the phenolic composition of leaf, flower and wood extracts of Piper imperiale, with antioxidant, antitubercular and cytotoxic activities was undertaken. The total phenol content ranged from 1.98 to 6.94 mg GAE/gDW among ethanolic extracts, and gallic acid, catechin, epicatechin, ferulic acid, resveratrol and quercetin were identified and quantified by HPLC. DPPH and ABTS assays showed high antioxidant activity of the leaf extract (EC50ABTS = 15.6 µg/mL, EC50DPPH = 27.3 µg/mL with EC50 in the same order of magnitude as the hydroxyquinone (EC50ABTS = 10.2 µg/mL, EC50DPPH = 15.7 µg/mL. The flower extract showed strong antimicrobial activity against Mycobacterium tuberculosis H37Rv. All the extracts exhibited dose-dependent cytotoxic effects against MCF-7 cancer cells. This is the first time that a Piper extract has been found to be highly active against M. tuberculosis. This study shows the biological potential of Piper imperiale extracts and gives way to bio-guided studies with well-defined biological activities.

  10. Antioxidant activities of the constituents of Picris echoides

    Directory of Open Access Journals (Sweden)

    MILUTIN STEFANOVIC

    2002-01-01

    Full Text Available Some flavonoids with antioxidant properties from the aerial parts of the plant species Picris echoides (family Asteraceae were identified. Upon chromatography, the ethyl acetate extract afforded flavonoids, such as: flavone apigenin (1 and its glucoside, cosmosiin (2, as well as common plant constituents from this family, flavonol galetin (3, 3,4’,5,6,7-pentahydroxyflavone and 4,4’,6,7-tetrahydroxyaurone (4. The structure of the aurone 4 has not been described so far in the literature and presented a very rare type of aurone skeleton. The structures of the isolated compounds were determined by interpretation of their physical and spectral data. The antioxidant activities of different extracts from Picris echoides were measured by the Schaal oven test at 60ºC and by the Rancimat method at 100ºC. Water/ethanol extracts (2:8, v/v, in concentrations of 0.02 and 0.05 %, showed lower activity than commercial tocopherol (Tch. On the contrary, the purified ethyl acetate extracts showed a strong concentration-dependent antioxidant effect. The investigation demonstrated that galetin was the main flavonol from this origin. According to the results of the two methods, galetin (3 showed a two-fold better activity than did Tch and a lower activity than did butylated hydroxyanisole (BHA. The aurone 4 exhibited significantly lower antioxidant activity than did galetin at the same concentration level. Thus, the plant species P. echoides is a new and favorable source of natural lipid antioxidants.

  11. Mechanochemical synthesis and antioxidant activity

    Indian Academy of Sciences (India)

    Curcumin, the active ingredient of turmeric, is a diaryl- heptanoid natural product that is endowed with much bioactivity;1 3 yet it has limited applications as a drug due to its low bioavailability.4 Structural modifi- cation of curcumin has been explored much as a strategy to circumvent this inadequacy. Among such structurally.

  12. Antioxidant activities and polyphenolic contents of three selected Micromeria species from Croatia.

    Science.gov (United States)

    Vladimir-Knežević, Sanda; Blažeković, Biljana; Štefan, Maja Bival; Alegro, Antun; Koszegi, Tamás; Petrik, József

    2011-02-10

    Antioxidant activities of three selected Micromeria species growing in Croatia (M. croatica, M. juliana and M. thymifolia) were evaluated using five different antioxidant assays, in comparison with plant polyphenolic constituents and reference antioxidants. All studied ethanolic extracts exhibited considerable activity to scavenge DPPH and hydroxyl free radicals, reducing power, iron chelating ability and total antioxidant capacity in the order: M. croatica > M. juliana > M. thymifolia. Total polyphenol (9.69-13.66%), phenolic acid (5.26-6.84%), flavonoid (0.01-0.09%) and tannin (3.07-6.48%) contents in dried plant samples were determined spectrophotometrically. A strong positive correlation between antioxidant activities and contents of phenolic acids and tannins was found, indicating their responsibility for effectiveness of tested plants. Our findings established Micromeria species as a rich source of antioxidant polyphenols, especially the endemic M. croatica.

  13. Antioxidant Activities and Polyphenolic Contents of Three Selected Micromeria Species from Croatia

    Directory of Open Access Journals (Sweden)

    Maja Bival Štefan

    2011-02-01

    Full Text Available Antioxidant activities of three selected Micromeria species growing in Croatia (M. croatica, M. juliana and M. thymifolia were evaluated using five different antioxidant assays, in comparison with plant polyphenolic constituents and reference antioxidants. All studied ethanolic extracts exhibited considerable activity to scavenge DPPH and hydroxyl free radicals, reducing power, iron chelating ability and total antioxidant capacity in the order: M. croatica > M. juliana > M. thymifolia. Total polyphenol (9.69–13.66%, phenolic acid (5.26–6.84%, flavonoid (0.01–0.09% and tannin (3.07–6.48% contents in dried plant samples were determined spectrophotometrically. A strong positive correlation between antioxidant activities and contents of phenolic acids and tannins was found, indicating their responsibility for effectiveness of tested plants. Our findings established Micromeria species as a rich source of antioxidant polyphenols, especially the endemic M. croatica.

  14. Antioxidative Activity of Tobacco Leaf Protein Hydrolysates

    Directory of Open Access Journals (Sweden)

    Guohua Rao

    2007-01-01

    Full Text Available Discarded tobacco leaf protein hydrolysate (DTLPH was prepared by enzymatic hydrolysis using papain and then separated using ultrafiltration (UF membranes with molecular mass cut-off (MMCO of 10, 5, 3 and 1 kDa. Four permeate fractions including 10-K, 5-K, 3-K and 1-K (the permeate fractions from 10, 5, 3 and 1 kDa hydrolysate fractions were obtained. The 5-K hydrolysate fraction had high oxidation inhibilitory ratio (42.62 %, which was about twofold higher than the original hydrolysate and as high as that of vitamin E (α-tocopherol. The fractionated hydrolysates were superior to the original hydrolysate in the antioxidative activity tested. Moreover, these separated hydrolysates showed the enhanced functional property. The amino acid composition of 5-K hydrolysate was analyzed and the results show that the high antioxidative activity of 5-K hydrolysate was derived from high content of histidine, methionine, cystine and tryptophan.

  15. Antioxidant activities of Rambutan (Nephelium lappaceum L peel in vitro

    Directory of Open Access Journals (Sweden)

    Mistriyani,

    2017-09-01

    Full Text Available Rambutan, Nephelium lappaceum L, peel due to consumption of fresh rambutan fruit is taken into account as waste, therefore the exploration of rambutan peel as a natural antioxidant is highly needed. The aim of this study is to investigate the antioxidant activity of rambutan peel from two cultivars (Aceh and Binjai using ABTS radical assay of and ferric reducing activity power (FRAP and to correlate with total phenolics and flavonoids. The powdered rambutan peel is extracted using maceration technique using methanol as extracting solvent. The methanolic extract is added with warm water and fractionated using petroleum ether, chloroform, and ethyl acetate to get corresponding fractions. Rambutan cultivar Binjai revealed the higher ABTS antiradical activity than that of cultivar Aceh. Furthermore, among methanolic extract and its fraction, ethyl acetate fraction exhibited the highest antiradical activity using ABTS radical with IC50 values of 3.10 μg/mL and 0.77 μg/mL for Aceh and Binjai, respectively. The ethyl acetate fraction also revealed the highest FRAP values of 1424.897 ± 28.56 μg/mg fraction sample (Aceh and 968.57± 7.48 μg/mg fraction sample (Binjai. These activities were correlated with phenolics and flavonoid contents. Rambutan peel exhibited strong antioxidant activities, contained high amounts of phenolics and flavonoid and is potential to be developed as a functional food.

  16. Chemical Composition, antioxidant activity, functional properties and ...

    African Journals Online (AJOL)

    Chemical Composition, antioxidant activity, functional properties and inhibitory action of unripe plantain ( M. Paradisiacae ) flour. ... of dry matter (48.00 ± 3.96%) and starch (31.10 ± 0.44%) but was low in phenol (1.42 ± 0.03%), protein (3.15 ± 0.042%), ash (5.50 ± 0.42%) and total soluble sugar (0.64 ± 0.001%) (p < 0.05).

  17. Physicochemical characterization and antioxidant activity of commercial Portuguese honeys.

    Science.gov (United States)

    Aazza, Smail; Lyoussi, Badiaa; Antunes, Dulce; Miguel, Maria Graça

    2013-08-01

    The present study evaluated the physicochemical characteristics and antioxidant activity of 13 commercial honeys from diverse floral origin, produced in Portugal. The values of electrical conductivity of cardoon and pennyroyal honeys were superior to the maximum limits defined by European legislation. Citrus, strawberry tree, and 1 sample of lavender honeys had values of diastase activity below those determined by European legislation. Strawberry tree, pennyroyal, and cardoon honeys had the highest amounts of potassium that coincided with the highest electrical conductivity. Strawberry tree honey was the most effective as antioxidant along with cardoon and heather honeys. This ability was strongly correlated with the amounts of phenols and flavonoids and not with the levels of vitamin C or proline. © 2013 Institute of Food Technologists®

  18. In Vitro Analysis of Antioxidant Activities of Oxalis Corniculata Linn ...

    African Journals Online (AJOL)

    In Vitro Analysis of Antioxidant Activities of Oxalis Corniculata Linn. Fractions in Various Solvents. D Ahmed, S Zara, H Baig. Abstract. As part of our search for natural antioxidants, this work presents an evaluation of antioxidant activities of methanolic extract of Oxalis corniculata and its sub-fractions in hexane, chloroform, ...

  19. Total phenolic, condensed tannin and antioxidant activity of four ...

    African Journals Online (AJOL)

    Different species of functional agricultural crops may vary in antioxidant capacities. In this study, the antioxidant activities of methanol extracts from four species of Carya genus were compared by various antioxidant assays, including the reducing power, 1,1-diphenyl-2-pycrylhydrazyl (DPPH) radical scavenging activity and ...

  20. Major factors influencing antioxidant contents and antioxidant activity in grapes and wines

    Directory of Open Access Journals (Sweden)

    Jaromír Lachman

    2009-03-01

    Full Text Available Jaromír Lachman, Miloslav Šulc, Katerina Faitová, Vladimír PivecDepartment of Chemistry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech RepublicAbstract: Phenolic compounds in wines, especially in red wines, possess strong antioxidant activity, have the largest effect in decreasing atherosclerosis by both hypolipemic and antioxidant mechanisms. The long-term uptake of red wine has a positive impact on antioxidant activity (AA of blood plasma in rats in vivo and increases AA by 15%–20% compared to a control group. In the article the effect of total phenolics (TP, total anthocyanins (TA, individual anthocyanins, procyanidins and phenolics contained in red grapes, musts, grape seeds and skins and wines on the AA is discussed. Significant impact of varieties, viticultural regions and locations, climate conditions and vintage has been shown. Likewise, the ways and individual stages of the vinification technology process, and storage conditions affect color, TP, TA, and AA and health aspects of produced wines. Resveratrol, another free radical scavenger mainly contained in the skins of grapes, inhibits the risk of cardiovascular diseases. Higher amounts of trans-resveratrol (RES have been found in wines from cool and wet climate regions and lesser amounts are typical for warm and dry regions. Changes in the TP content and AA affected by grape variety, vineyard location and winemaking process in white and blue varieties from different vineyards of the Czech Republic were studied. Significant differences in TP among varieties were found. Analysis of variance showed statistically high differences among red and white wines and growing locations. Wines differed significantly in TP content and AA increased significantly during the winemaking process. Statistically significant differences in AA values were found among growing areas, wines and varieties. Significant positive correlations between TP

  1. GC-MS analysis of the antioxidant active fractions of Micromeria juliana with anticholinesterase activity.

    Science.gov (United States)

    Oztürk, Mehmet; Kolak, Ufuk; Duru, Mehmet Emin; Harmandar, Mansur

    2009-09-01

    The aerial parts of Micromeria juliana (L.) Bentham ex Reichb. were extracted with light petroleum, acetone and methanol, successively. The antioxidant activity of different concentrations of the extracts was evaluated using different antioxidant tests, namely total antioxidant (lipid peroxidation inhibition activity), DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, ferric reducing power, and metal chelating. Total antioxidant activity was determined using the beta-carotene-linoleic acid assay. Unexpectedly, the light petroleum extract exhibited strong lipid peroxidation inhibition activity. The extract was fractionated on a silica gel column and the antioxidant activity of the fractions was determined by the beta-carotene-linoleic assay at 25 microg/mL concentration. The fractions that exhibited more than 50% inhibition activity were analysed by GC and GC/MS; thus, the structure of fourteen compounds were elucidated. In addition, acetyl- and butyryl-cholinesterase inhibitory activities of the extracts were also determined in vitro. The light petroleum and acetone extracts were found to have mild butyrylcholinesterase inhibitory activity.

  2. In vitro and in vivo antioxidant activities of inulin.

    Science.gov (United States)

    Shang, Hong-Mei; Zhou, Hai-Zhu; Yang, Jun-Yan; Li, Ran; Song, Hui; Wu, Hong-Xin

    2018-01-01

    This study was designed to investigate the in vitro and in vivo antioxidant activities of inulin. The in vitro assays demonstrated that the antioxidant activities of inulin, including the DPPH radical scavenging activity, ABTS scavenging activity and ferric reducing power, were weak and significantly lower than those of Vitamin C (P inulin on the antioxidant status of laying hens was evaluated with in vivo antioxidant assays. The results indicated that inulin supplementation quadratically improved the egg production rate of the laying hens (P inulin levels increased (P inulin levels increased (P inulin has the potential to improve the antioxidant status of laying hens.

  3. Antioxidant and Antiproliferative Activity ofAsparagopsis taxiformis.

    Science.gov (United States)

    Neethu, P V; Suthindhiran, K; Jayasri, M A

    2017-01-01

    Asparagopsis taxiformis ( Rhodophyta ) is a species of red algae belonging to the family Bonnemaisoniaceae . The objective of the present study was to evaluate antioxidant and antiproliferative activity of four fractions (petroleum ether, chloroform, ethyl acetate, and methanol) of A. taxiformis . The red seaweed, A. taxiformis was collected from Mandapam Coastal Region, Gulf of Mannar, Tamil Nadu. Epiphytes present in algal extracts were cleaned and washed with seawater and fresh water. In vitro antioxidant activity was determined by hydrogen peroxide scavenging, ferric reducing antioxidant power, superoxide radical, metal-chelating activity, and phosphomolybdenum reduction assay. Further, the cytotoxic activity was evaluated using brine shrimp lethality assay. This method is rapid, reliable, inexpensive, and convenient as compared to other cytotoxicity assays. Gallic acid, ethylenediaminetetraacetic acid, ascorbic acid, and quercetin were used as reference antioxidant compounds. Reducing power of chloroform extract increased with increasing concentration of the extract. The radical scavenging activity of extracts was in the following order: ascorbic acid > methanol > chloroform > petroleum ether > ethyl acetate. Highest metal-chelating activity was observed in petroleum ether fractions (63%). Reduction of Mo (VI) to Mo (V) increased in methanol extract (27%) at 100 μg/ml. Moreover, all fractions had an inhibitory effect on the formation of hydroxyl radicals. Results showed that ethyl acetate, methanol, and petroleum ether fractions exhibited potent cytotoxic activity with median lethal concentration values of 84.33, 104.4, and 104.4 μg/ml, respectively. Thus, the results showed that red algae possess strong antioxidant and cytotoxic activity that suggests their possible use in the development of pharmaceutical drugs. Various fractions of red algae Asparagopsis taxiformis was evaluated for in vitro antioxidant and antiproliferative studies. All results indicate

  4. [Antioxidant activity of cationic whey protein isolate].

    Science.gov (United States)

    titova, M E; Komolov, S A; Tikhomirova, N A

    2012-01-01

    The process of lipid peroxidation (LPO) in biological membranes of cells is carried out by free radical mechanism, a feature of which is the interaction of radicals with other molecules. In this work we investigated the antioxidant activity of cationic whey protein isolate, obtained by the cation-exchange chromatography on KM-cellulose from raw cow's milk, in vitro and in vivo. In biological liquids, which are milk, blood serum, fetal fluids, contains a complex of biologically active substances with a unique multifunctional properties, and which are carrying out a protective, antimicrobial, regenerating, antioxidant, immunomodulatory, regulatory and others functions. Contents of the isolate were determined electrophoretically and by its biological activity. Cationic whey protein isolate included lactoperoxidase, lactoferrin, pancreatic RNase, lysozyme and angeogenin. The given isolate significantly has an antioxidant effect in model experimental systems in vitro and therefore may be considered as a factor that can adjust the intensity of lipid oxidation. In model solutions products of lipid oxidation were obtained by oxidation of phosphatidylcholine by hydrogen peroxide in the presence of a source of iron. The composition of the reaction mixture: 0,4 mM H2O2; 50 mcM of hemin; 2 mg/ml L-alpha-phosphatidylcholine from soybean (Sigma, German). Lipid peroxidation products were formed during the incubation of the reaction mixture for two hours at 37 degrees C. In our studies rats in the adaptation period immediately after isolation from the nest obtained from food given orally native cationic whey protein isolate at the concentration three times higher than in fresh cow's milk. On the manifestation of the antioxidant activity of cationic whey protein isolate in vivo evidence decrease of lipid peroxidation products concentration in the blood of rats from the experimental group receipt whey protein isolate in dos 0,6 mg/g for more than 20% (pisolate has an antioxidant

  5. Effect of extraction solvents on polyphenols and antioxidant activity of medicinal halophytes

    International Nuclear Information System (INIS)

    Qasim, M.; Aziz, I.; Gul, B.

    2016-01-01

    This study was conducted to determine the most effective solvent for extraction of polyphenols and antioxidant activity of medicinally important coastal halophytes (Thespesia populneoides, Salvadora persica, Ipomoea pes-caprae, Suaeda fruticosa and Pluchea lanceolata) known for high antioxidant potential. Five different solvents (water, 80% methanol, 80% ethanol, acetone and chloroform) were used to quantify polyphenols including total phenolic (TPC), total flavonoid (TFC) and proanthocyanidin contents (PC) and antioxidant capacity using DPPH radical scavenging and Ferric reducing antioxidant power (FRAP) activities. Among solvents of different polarities 80% methanol appeared most effective for polyphenol extraction. Thespesia populneoides had the highest polyphenols (TPC, TFC and PC) followed by Salvadora persica. Highest antioxidant activity was also found in T. populneoides and S. persica using the same solvent (80% methanol) which appeared better than synthetic antioxidants (BHA and BHT). The correlation analyses of each solvent showed strong to weak relationships among all studied parameters with maximum values (r and R2) in methanol followed by ethanol and water. Weaker correlation of acetone and chloroform indicates low capacity of these solvents both for polyphenol extraction and antioxidant activity. Our results reveal that aqueous methanol extracts of coastal halophytes had comparatively higher antioxidant activity than commercial antioxidants which indicate both their prospective efficacy and potential to replace synthetic derivatives from edible and medicinal products. (abstract)

  6. Antioxidant activity and phenolic content of leaf infusions of Myrtaceae species from Cerrado (Brazilian Savanna).

    Science.gov (United States)

    Takao, L K; Imatomi, M; Gualtieri, S C J

    2015-11-01

    There is considerable interest in identifying new antioxidants from plant materials. Several studies have emphasized the antioxidant activity of species belonging to the Myrtaceae family. However, there are few reports on these species from the Cerrado (Brazilian savanna). In this study, the antioxidant activity and phenolic content of 12 native Myrtaceae species from the Cerrado were evaluated (Blepharocalyx salicifolius, Eugenia bimarginata, Eugenia dysenterica, Eugenia klotzschiana, Hexachlamys edulis, Myrcia bella, Myrcia lingua, Myrcia splendens, Myrcia tomentosa, Psidium australe, Psidium cinereum, and Psidium laruotteanum). Antioxidant potential was assessed using the antioxidant activity index (AAI) by the DPPH method and total phenolic content (TPC) by the Folin-Ciocalteu assay. There was a high correlation between TPC and AAI values. Psidium laruotteanum showed the highest TPC (576.56 mg GAE/g extract) and was the most potent antioxidant (AAI = 7.97, IC50 = 3.86 µg·mL-1), with activity close to that of pure quercetin (IC50 = 2.99 µg·mL-1). The extracts of nine species showed IC50 of 6.24-8.75 µg·mL-1. Most species showed TPC and AAI values similar to or higher than those for Camellia sinensis, a commonly consumed tea with strong antioxidant properties. The results reveal that the analyzed Myrtaceae species from the Cerrado possess high phenolic contents and antioxidant activities. Thus, they are a potential source of new natural antioxidants.

  7. In vitro and in vivo antioxidant activities of inulin.

    Directory of Open Access Journals (Sweden)

    Hong-Mei Shang

    Full Text Available This study was designed to investigate the in vitro and in vivo antioxidant activities of inulin. The in vitro assays demonstrated that the antioxidant activities of inulin, including the DPPH radical scavenging activity, ABTS scavenging activity and ferric reducing power, were weak and significantly lower than those of Vitamin C (P < 0.05. The influence of dietary supplementation with inulin on the antioxidant status of laying hens was evaluated with in vivo antioxidant assays. The results indicated that inulin supplementation quadratically improved the egg production rate of the laying hens (P < 0.01. The antioxidant enzyme activities in the serum, including SOD, CAT, and GSH-Px, and the total antioxidant capacity increased quadratically as inulin levels increased (P < 0.001. The levels of MDA in the serum decreased quadratically as inulin levels increased (P < 0.001. These findings suggest that inulin has the potential to improve the antioxidant status of laying hens.

  8. Polyphenol content and antioxidant activity of fourteen wild edible fruits from Burkina Faso.

    Science.gov (United States)

    Lamien-Meda, Aline; Lamien, Charles Euloge; Compaoré, Moussa M Y; Meda, Roland N T; Kiendrebeogo, Martin; Zeba, Boukare; Millogo, Jeanne F; Nacoulma, Odile G

    2008-03-06

    A total of fourteen (14) species of wild edible fruits from Burkina Faso were analyzed for their phenolic and flavonoid contents, and their antioxidant activities using the DPPH, FRAP and ABTS methods. The data obtained show that the total phenolic and total flavonoid levels were significantly higher in the acetone than in the methanol extracts.Detarium microcarpum fruit had the highest phenolic and the highest flavonoid content,followed by that of Adansonia digitata, Ziziphus mauritiana, Ximenia americana and Lannea microcarpa. Significant amounts of total phenolics were also detected in the other fruit species in the following order of decreasing levels: Tamarindus indica > Sclerocaryabirrea > Dialium guineense > Gardenia erubescens > Diospyros mespiliformis > Parkiabiglobosa > Ficus sycomorus > Vitellaria paradoxa. Detarium microcarpum fruit also showed the highest antioxidant activity using the three antioxidant assays. Fruits with high antioxidant activities were also found to possess high phenolic and flavonoid contents. There was a strong correlation between total phenolic and flavonoid levels and antioxidant activities.

  9. Antioxidant and Anticancer activities of yeast grown on commercial ...

    African Journals Online (AJOL)

    Antioxidant and Anticancer activities of yeast grown on commercial media. Amal S. Shahat. Abstract. The use of Synthetic antioxidants is being widely restricted because of their reported toxic and carcinogenic effects. Thus, now there is considerable interest being focused towards finding antioxidants from natural sources ...

  10. Isoflavone content and antioxidant activity of Thai fermented ...

    African Journals Online (AJOL)

    USER

    2010-06-28

    Jun 28, 2010 ... Value in table expressed as mean ± SD. Means in each column with different letters are significantly different (p < 0.05). 1 TEAC: Trolox equivalent antioxidant capacity; 2 FRAP: Ferric reducing antioxidant power. Table 3. Weight variation, disintegration, antioxidant activity, and isoflavone contents of hard ...

  11. Antioxidant activities in extracts of selected indigenous vegetables ...

    African Journals Online (AJOL)

    Antioxidant activities in extracts of selected indigenous vegetables from Kenya and Malawi. ... intake of these vegetables species in sufficient concentrations should thus be recommended to enhance an optimal antioxidant capacity in the body. Keywords: Antioxidants, free radicals, health benefits, indigenous vegetables

  12. Chemical profiling and antioxidant activity of Bolivian propolis.

    Science.gov (United States)

    Nina, Nélida; Quispe, Cristina; Jiménez-Aspee, Felipe; Theoduloz, Cristina; Giménez, Alberto; Schmeda-Hirschmann, Guillermo

    2016-04-01

    Propolis is a relevant research subject worldwide. However, there is no information so far on Bolivian propolis. Ten propolis samples were collected from regions with high biodiversity in the main honey production places in Bolivia and were analyzed for their total phenolics (TP), flavonoids (TF) and antioxidant activity. The chemical profiles of the samples were assessed by TLC, HPLC-DAD, HPLC-DAD-MS/MS(n) and NMR analysis. TP, TF, TLC and NMR analysis showed significant chemical differences between the samples. Isolation of the main constituents by chromatography and identification by HPLC-DAD-MS/MS(n) achieved more than 35 constituents. According to their profiles, the Bolivian propolis can be classified into phenolic-rich and triterpene-rich samples. Propolis from the valleys (Cochabamba, Chuquisaca and Tarija) contained mainly prenylated phenylpropanoids, while samples from La Paz and Santa Cruz contained cycloartane and pentacyclic triterpenes. Phenolic-rich samples presented moderate to strong antioxidant activity while the triterpene-rich propolis were weakly active. High chemical diversity and differential antioxidant effects were found in Bolivian propolis. Our results provide additional evidence on the chemical composition and bioactivity of South American propolis. © 2015 Society of Chemical Industry.

  13. In Vitro and In Vivo Antioxidant Activity of Aged Ginseng (Panax ginseng).

    Science.gov (United States)

    Chung, Soo Im; Kang, Mi Young; Lee, Sang Chul

    2016-03-01

    Fresh ginseng roots were aged in an oven at 80°C for 14 d. The in vitro and in vivo antioxidant activities of this aged ginseng, in comparison with those of the white and red ginsengs, were evaluated. In in vitro antioxidant assays, the ethanolic extracts from aged ginseng showed significantly higher free radical scavenging activity and reducing power than those of the white and red ginsengs. In in vivo antioxidant assays, mice were fed a high fat diet supplemented with white, red, or aged ginseng powders. High fat feeding resulted in a significant increase in lipid peroxidation and a substantial decrease in antioxidant enzymes activities in the animals. However, diet supplementation of ginseng powders, particularly aged ginseng, markedly reduced lipid peroxidation and enhanced the antioxidant enzymes activities. The results illustrate that the aged ginseng has greater in vitro and in vivo antioxidant capacity than the white and red ginsengs. The aged ginseng also showed considerably higher total saponin, phenolic, and flavonoid contents, indicating that its antioxidant capacity may have been partly due to its high levels of antioxidant compounds. This new ginseng product may be useful as a functional food with strong antioxidant potential.

  14. Scuba diving activates vascular antioxidant system.

    Science.gov (United States)

    Sureda, A; Batle, J M; Ferrer, M D; Mestre-Alfaro, A; Tur, J A; Pons, A

    2012-07-01

    The aim was to study the effects of scuba diving immersion on plasma antioxidant defenses, nitric oxide production, endothelin-1 and vascular endothelial growth factor levels. 9 male divers performed an immersion at 50 m depth for a total time of 35 min. Blood samples were obtained before diving at rest, immediately after diving, and 3 h after the diving session. Leukocyte counts, plasma 8oxoHG, malondialdehyde and nitrite levels significantly increased after recovery. Activities of lactate dehydrogenase, creatine kinase, catalase and superoxide significantly increased immediately after diving and these activities remained high after recovery. Plasma myeloperoxidase activity and protein levels and extracellular superoxide dismutase protein levels increased after 3 h. Endothelin-1 concentration significantly decreased after diving and after recovery. Vascular endothelial growth factor concentration significantly increased after diving when compared to pre-diving values, returning to initial values after recovery. Scuba diving at great depth activated the plasma antioxidant system against the oxidative stress induced by elevated pO₂ oxygen associated with hyperbaria. The decrease in endothelin-1 levels and the increase in nitric oxide synthesis could be factors that contribute to post-diving vasodilation. Diving increases vascular endothelial growth factor plasma levels which can contribute to the stimulation of tissue resistance to diving-derived oxidative damage. © Georg Thieme Verlag KG Stuttgart · New York.

  15. Effects of extraction methods of phenolic compounds from Xanthium strumarium L. and their antioxidant activity

    Directory of Open Access Journals (Sweden)

    R. Scherer

    2014-03-01

    Full Text Available The effect of extraction methods and solvents on overall yield, total phenolic content, antioxidant activity, and the composition of the phenolic compounds in Xanthium strumarium extracts were studied. The antioxidant activity was determined by using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH, and the composition of the phenolic compounds was determined by HPLC-DAD and LC/MS. All results were affected by the extraction method, especially by the solvent used, and the best results were obtained with the methanol extract. The methanolic and ethanolic extracts exhibited strong antioxidant activity, and the chlorogenic and ferulic acids were the most abundant phenolic compounds in the extracts.

  16. Identification of major phenolic compounds from Nephelium lappaceum L. and their antioxidant activities.

    Science.gov (United States)

    Thitilertdecha, Nont; Teerawutgulrag, Aphiwat; Kilburn, Jeremy D; Rakariyatham, Nuansri

    2010-03-09

    Nephelium lappaceum is a tropical fruit whose peel possesses antioxidant properties. Experiments on the isolation and identification of the active constituents were conducted, and on their antioxidant activity using a lipid peroxidation inhibition assay. The methanolic extract of N. lappaceum peels exhibited strong antioxidant properties. Sephadex LH-20 chromatography was utilized in the isolation of each constituent and the antioxidant properties of each was studied. The isolated compounds were identified as ellagic acid (EA) (1), corilagin (2) and geraniin (3). These compounds accounted for 69.3% of methanolic extract, with geraniin (56.8%) as the major component, and exhibited much greater antioxidant activities than BHT in both lipid peroxidation (77-186 fold) and DPPH* (42-87 fold) assays. The results suggest that the isolated ellagitannins, as the principal components of rambutan peels, could be further utilized as both a medicine and in the food industry.

  17. Identification of Major Phenolic Compounds from Nephelium lappaceum L. and Their Antioxidant Activities

    Directory of Open Access Journals (Sweden)

    Nuansri Rakariyatham

    2010-03-01

    Full Text Available Nephelium lappaceum is a tropical fruit whose peel possesses antioxidant properties. Experiments on the isolation and identification of the active constituents were conducted, and on their antioxidant activity using a lipid peroxidation inhibition assay. The methanolic extract of N. lappaceum peels exhibited strong antioxidant properties. Sephadex LH-20 chromatography was utilized in the isolation of each constituent and the antioxidant properties of each was studied. The isolated compounds were identified as ellagic acid (EA (1, corilagin (2 and geraniin (3. These compounds accounted for 69.3% of methanolic extract, with geraniin (56.8% as the major component, and exhibited much greater antioxidant activities than BHT in both lipid peroxidation (77-186 fold and DPPH• (42-87 fold assays. The results suggest that the isolated ellagitannins, as the principal components of rambutan peels, could be further utilized as both a medicine and in the food industry.

  18. Antioxidant and antimicrobial activities of helinus lanceolatus

    International Nuclear Information System (INIS)

    Ajaib, M.

    2015-01-01

    The extracts of petroleum ether, chloroform, methanol and aqueous of Helinus lanceolatus were tested for their antioxidant potential, antibacterial and antifungal activities. The results revealed that the methanolic extract showed the highest zone of inhibition 50 ± 1.15 mm against Escherichia coli and aqueous extract shows excellent inhibition for fungi Aspergillus niger 17 ± 2.6 mm. The water extract showed highest DPPH radical scavenging activity i.e. 91.8 - 0.0.09% at a concentration of 500 mu g/ml with IC50 value 12.29 ± 0.59 micro g/ml relative to butylated hydroxyltoluene (BHT) having IC50 value 12.52 ± 0.89 micro g/ml. Chloroform extract showed highest antioxidant activity 0.840 ± 0.13 micro g/ml relative to standard 0.891 ± 0.13 micro g/ml while the highest FRAP value i.e. 90.66 ± 4.54 TE micro g/ml was shown by petroleum ether fraction. Methanolic extract also showed good value of inhibition of lipid peroxidation, i.e. 59.11 ± 0.12%. (author)

  19. Antioxidant activities of Barringtonia racemosa leaves

    Directory of Open Access Journals (Sweden)

    Nurul Syafizan, R.

    2017-12-01

    Full Text Available Proximate analysis of Barringtonia racemosa (putat leaves was carried out and total phenolic content (TPC was measured using total phenolic assay, also known as the Folin Ciocalteu (FC assay. Antioxidant activities of the methanolic and aqueous extracts were evaluated by ferric reducing antioxidant power (FRAP assay; the free radical scavenging capacity was determined by 1,1-diphenyl-1-picrylhydrazyl radical scavenging capacity (DPPH assay. Proximate analysis showed that the leaves contained 70.52% moisture, 0.96% ash, 2.54% protein, 0.26% fat, 1.38% crude fiber, and 25.71% carbohydrates. The TPC amount exhibited by the methanolic extract was 1585.20±25.54 mg GAE/100 g sample, which was higher compared with the aqueous plant extract with 1314.12±66.19 mg GAE/100 g (p<0.05. Significant (p<0.05 difference in terms of the flavonoid content was observed between the extracts obtained by methanolic and aqueous extraction. Total condensed tannin content showed significant (p<0.05 difference between the methanolic and the aqueous extracts, which were 33.11±0.61 mg CE/100 g and 13.38±0.87 mg CE/100 g, respectively. Methanolic extract of Barringtonia racemosa leaves showed a higher DPPH scavenging activity than the aqueous extract. FRAP value for the methanolic extract of the sample was higher than the aqueous extract, with a significant difference (p<0.05. Results of this study will provide a platform for the future paradigm of these potential natural sources of antioxidants in food and nutraceutical applications.

  20. Polysaccharides in Sipunculus nudus: Extraction condition optimization and antioxidant activities

    Science.gov (United States)

    Zhang, Qin; Dong, Lanfang; Tong, Tong; Wang, Qingchao; Xu, Mingzhu

    2017-02-01

    Marine organisms constitute unlimited resource of bioactive substances due to their high biodiversity and represent a valuable source of new compounds. This study optimized the alkali-extraction conditions and antioxidant activities of soluble polysaccharides from the body wall of Sipunculus nudus. The effects of solid-liquid ratio, extraction duration, extraction temperature, and alkali concentration on the yield of S. nudus polysaccharides (SNP) were examined, according to which the optimal combination of extraction parameters was obtained by an orthogonal test. The relative influencing importance of different extraction parameters on the yield of SNP followed the order as solid-liquid ratio > extraction temperature > alkali concentration > extraction duration. The highest extraction yield, 1.98%, was achieved under an optimal extraction condition: temperature 60°C, solid-liquid ratio 1:6 g mL-1, duration 5 h, and alkali (NaOH) mass fraction 6%. The in vitro antioxidant activities examination showed that extracted SNP under this optimized condition had strong power in reducing certain hydroxyl and superoxide radical scavenging abilities. The promising results showed that extracted SNP could be a potent natural antioxidant.

  1. Degradation and the antioxidant activity of polysaccharide from Enteromorpha linza.

    Science.gov (United States)

    Zhang, Zhongshan; Wang, Xiaomei; Mo, Xiaofang; Qi, Huimin

    2013-02-15

    Polysaccharide extracted from Enteromorpha linza possesses excellent antioxidant activities, but its molecular weight was greatly high which influences the activity. In this study, the combination of ascorbic acid and H(2)O(2) was used as degradation reagents in order to obtain the lower molecular weight product. The results suggested that the most effective molar ratio of the two reagents was 1. Three degraded polysaccharides were selected to evaluate their antioxidant activities in vitro and characterized the relationship between antioxidant activity and chemical characteristics. It was found that the degraded sample with lower molecular weight possessed the higher antioxidant activities. Copyright © 2012 Elsevier Ltd. All rights reserved.

  2. Antioxidant and Anti-Fatigue Activities of Flavonoids from Puerariae ...

    African Journals Online (AJOL)

    This study evaluated the antioxidant and anti-fatigue activities of flavonoids from Puerariae radix (FPR). In vitro antioxidant activities of FPR were investigated through hydroxyl and superoxide radical scavenging activities. In vivo anti-fatigue activity of FPR was investigated through loaded swimming exercise of mice. Results ...

  3. A comparative study on the antioxidant activity of fringe tree ...

    African Journals Online (AJOL)

    Administrator

    2007-02-19

    Feb 19, 2007 ... reducing activity, hydrogen peroxide (H2O2) scavenging and ferrous metal chelating activities were for used antioxidant evaluation of MEFT and EEFT. The both extracts neutralized the activities of radicals and inhibited the peroxidation reactions of linoleic acid emulsion. Total antioxidant activity was.

  4. Antioxidant activity of extracts obtained from residues of different oilseeds.

    Science.gov (United States)

    Matthäus, B

    2002-06-05

    Residues of the oil-extracting process of oilseeds contain bioactive substances such as phenolic compounds, which could be used as natural antioxidants for the protection of fats and oils against oxidative deterioration. Thus, the extraction of such constituents from residual material can be considered to contribute to the added value of these residues, which could justify their isolation. In the present work the fat-free residues of eight different oilseeds whose oils are usable for nutritional applications, and also as renewable resources, were extracted with 70% methanol, 70% acetone, water, and ethyl acetate/water, respectively. The resulting extracts were investigated regarding their content of total phenolic compounds by the Folin-Ciocalteau assay, sinapine, flavanoids, and the UV-absorption spectra. Further, the antioxidant activity of the extracts was characterized by the DPPH method, the beta-carotene-linoleic acid assay, and ESR investigations. The fat-free residues of the different oilseeds contained considerable amounts of extractable substances. The yields decreased with decreasing polarity of the solvent in the order water, 70% methanol, 70% acetone, and ethyl acetate. The ratio of total phenolic compounds to the extractable compounds ranged from 3 to 19%. There was no significant correlation between the amount of total extractable compounds and the total phenolic compounds (p < 0.001). All extracts showed remarkable antioxidant activities determined with the different methods. The effects depended strongly on the solvent used for the extraction as well as on the extracted residue. A correlation between the methods used for the characterization of the antioxidant activity and the composition of the residues could not be shown.

  5. Antioxidant activity of medicinal plant polysaccharides.

    Science.gov (United States)

    Kardosová, A; Machová, E

    2006-07-01

    Eleven polysaccharides have been isolated from the leaves of Arctium lappa var. herkules, Aloe barbadensis, Althaea officinalis var. robusta, Plantago lanceolata var. libor, aerial parts and roots of Rudbeckia fulgida var. sullivantii, stems of Mahonia aquifolium, and peach-tree (Prunus persica) gum exudates. The polysaccharides were investigated for their ability to inhibit peroxidation of soyabean lecithin liposomes by OH radicals. The highest inhibition was found with glucuronoxylans of A. officinalis var. robusta and P. lanceolata var. libor, aerial parts. Their antioxidant activity accounted for approximately 69% of the activity of the reference compound alpha-tocopherol. The activity of eight polysaccharides ranged from 20 to 45%, while the fructofuranan from P. lanceolata var. libor roots was practically inactive.

  6. In vitro antimicrobial activity on clinical microbial strains and antioxidant properties of Artemisia parviflora

    Directory of Open Access Journals (Sweden)

    Ahameethunisa Abdul R

    2012-11-01

    Full Text Available Abstract Background Artemisia parviflora leaf extracts were evaluated for potential antimicrobial and antioxidant properties. Antimicrobial susceptibility assay was performed against ten standard reference bacterial strains. Antioxidant activity was analyzed using the ferric thiocyanate and 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH assays. Radical scavenging activity and total phenolic content were compared. Phytochemical analyses were performed to identify the major bioactive constitution of the plant extract. Results Hexane, methanol and ethyl acetate extracts of A. parviflora leaves exhibited good activity against the microorganisms tested. The n-hexane extract of A. parviflora showed high inhibition of the growth of Pseudomonas aeruginosa, Escherichia coli and Shigella flexneri. Methanol extract showed strong radical scavenging and antioxidant activity, other extracts showed moderate antioxidant activity. The major derivatives present in the extracts are of terpenes, steroids, phenols, flavonoids, tannins and volatile oil. Conclusions The results obtained with n-hexane extract were particularly significant as it strongly inhibited the growth of P. aeruginosa, E. coli and S. flexneri. The major constituent of the n-hexane extract was identified as terpenes. Strong antioxidant activity could be observed with all the individual extracts. The antimicrobial and antioxidant property of the extracts were attributed to the secondary metabolites, terpenes and phenolic compounds present in A. parviflora and could be of considerable interest in the development of new drugs.

  7. Extraction and antioxidant activity of polysaccharides from Rana chensinensis skin.

    Science.gov (United States)

    Wang, Zhanyong; Zhao, Yuanyuan; Su, Tingting

    2015-01-22

    The extraction process of polysaccharides from Rana chensinensis skin was optimized by using a Box-Behnken design. The optimum extraction conditions were as follows: extraction time, 4.96h; extraction temperature, 100°C; ratio of water to raw material, 60; and extraction frequency, 1. Under these conditions, the experimental polysaccharide yield was 2.03±0.14%, which agreed with the predicted yield. The purified polysaccharide RCSP II was successfully obtained by diethylaminoethanol-Sepharose and Sepharose CL-6B column chromatography. In vitro experiments showed that RCSP II exhibited a strong scavenging activity against superoxide anion and 1,1-diphenyl-2-picrylhydrazyl radicals but a weak scavenging activity against hydroxyl radicals. RCSP II also showed a strong reducing capacity. Thus, this polysaccharide can be used as a natural antioxidant in functional foods or medicines. Copyright © 2014 Elsevier Ltd. All rights reserved.

  8. Antioxidant activity of Vitex agnus-castus L. extracts.

    Science.gov (United States)

    Sağlam, Hüsniye; Pabuçcuoğlu, Aysun; Kivçak, Bijen

    2007-11-01

    The ethanol, n-hexane and water extracts of Vitex agnus-castus L. leaves and fruits were screened for antioxidant activity. The antioxidant activity of plant extracts was determined by an improved assay based on the decolorization of the radical monocation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS.+). The water and ethanol extracts showed stronger antioxidant activity than the n-hexane extracts. Copyright (c) 2007 John Wiley & Sons, Ltd.

  9. Antioxidant Activity and Bioactive Components of Oxalidaceae Fruit Extracts

    International Nuclear Information System (INIS)

    Asna, N.A.; Noriham, A.

    2014-01-01

    had the highest value. There were strong positive correlations between antioxidant activity assays and TPC or TFC, hence indicating that the four types of Oxalidaceae fruits used in this study have the potential as natural antioxidant. (author)

  10. In vitro antioxidant activity of Vetiveria zizanioides root extract

    African Journals Online (AJOL)

    lmboera

    suitable antioxidants such as butyl hydroxy toluene, ascorbic acid, quercetin, alpha tocopherol, pyrocatechol and curcumin respectively. The generation of free radicals O2-, H2O2, OH and NO were effectively scavenged by the ethanolic extract of V.zizanioides. In all these methods, the extract showed strong antioxidant ...

  11. Antioxident activity of the mangrove endophytic fungus (Trichoderma sp.

    Directory of Open Access Journals (Sweden)

    Saravanakumar Kandasamy

    2014-07-01

    Full Text Available Objective: To test antioxidant property of the endophytic Trichoderma species isolated from the leaves of 12 mangroves of Andaman Nicobar Islands. Methods: Eight strains of Trichoderma species were found predominant and their crude extracts were assessed for antioxidant activity by using seven assays. Results: Total antioxidant activity varied with the strains and it was maximum in Trichoderma EMFCAS8 and other strains also showed considerable activity. Conclusions: This work concluded that mangroves are rich in endophytic Trichoderma species with potential for antioxidant activity.

  12. Antioxidant and antibacterial activities of different plants extracts ...

    African Journals Online (AJOL)

    Antioxidant and antibacterial activities of different plants extracts against Staphylococcus aureus isolated from soccer player's shoes and knowledge and applications about foot hygiene of the soccer players.

  13. Novel Indole-Based Analogs of Melatonin: Synthesis and in Vitro Antioxidant Activity Studies

    Directory of Open Access Journals (Sweden)

    Hanif Shirinzadeh

    2010-03-01

    Full Text Available The aim of this study was to synthesize and examine possible in vitro antioxidant effects of indole-based melatonin analogue compounds. As a part of our ongoing study nineteen indole hydrazide/hydrazone derivatives were synthesized, characterized and their in vitro antioxidant activity was investigated by three different assays: by evaluating their reducing effect against oxidation of a redox sensitive fluorescent probe, by examining their protective effect against H2O2-induced membrane lipid peroxidation and by determining their inhibitory effect on AAPH–induced hemolysis of human erythrocytes. The results indicated significant strong antioxidant activity for most of the compounds, when compared to melatonin.

  14. Role of paramagnetic polyconjugated clusters in lignin antioxidant activity (in vitro)

    International Nuclear Information System (INIS)

    Dizhbite, T; Ponomarenko, J; Andersone, A; Dobele, G; Lauberts, M; Krasilnikova, J; Telysheva, G; Mironova-Ulmane, N

    2012-01-01

    Using physico-chemical methods (EPR, SEC, Py-GC/MS and UV/VIS spectroscopy) and wet chemical analysis, the characteristics of 6 hardwood lignins in terms of functionality, molecular weight and composition of lignin substructures were determined and considered together with the results of DPPH., ABTS. + and O 2 . − antioxidant assays with the aim to understand the relationships governing antioxidant properties of lignin. The strong positive linear correlation between lignin antioxidant capacity in the three assays used and the extent of conjugation of paramagnetic polyconjugated clusters in lignin macromolecules was found. The biological activity of the most active alkaline lignins was assessed by in vitro experiment with human blood.

  15. Tetrahydrofolate and 5-methyltetrahydrofolate are folates with high antioxidant activity. Identification of the antioxidant pharmacophore.

    NARCIS (Netherlands)

    Rezk, BM; Haenen, GR; Vijgh, van der W.J.F.

    2003-01-01

    The presumed protective effect of folic acid on the pathogenesis of cardiovascular, hematological and neurological diseases and cancer has been associated with the antioxidant activity of folic acid. Peroxynitrite (PON) scavenging activity and inhibition of lipid peroxidation (LPO) of the

  16. Phenolic compounds and antioxidant activity of bulb extracts of six Lilium species native to China.

    Science.gov (United States)

    Jin, Lei; Zhang, Yanlong; Yan, Linmao; Guo, Yulong; Niu, Lixin

    2012-08-03

    Lily (Lilium) is used as an important edible and medical plant species with a vague taxonomic classification and a long history in China. Bulbs of six Lilium species (L. regale, L. concolor, L. pumilum, L. leucanthum, L. davidii var. unicolor and L. lancifolium) native to China were investigated with a view to their exploitation as a potential source of natural antioxidants due to their phenolic composition and dietary antioxidant potential. The results showed that all bulb extracts exhibited strong antioxidant activities, which generally correlated positively with the total phenolic contents (r = 0.68 to 0.94), total flavonoid contents (r = 0.51 to 0.89) and total flavanol contents (r = 0.54 to 0.95). High-performance liquid chromatography (HPLC) analysis revealed that rutin and kaempferol were the major phenolic components in the extracts. Hierarchical cluster analysis showed that L. regale belonged to the group with high phenolic content and strong antioxidant power. L. concolor and L. pumilum were arranged in one group characterized by moderate phenolic content and antioxidant capacity, while L. leucanthum, L. davidii var. unicolor and L. lancifolium were clustered in the third group with low phenolic content and weak antioxidant activity. These strongly suggest that lily bulbs may serve as a potential source of natural antioxidant for food and pharmaceutical applications.

  17. Anti-Inflammatory and Antioxidant Activities of Methanol Extracts and ...

    African Journals Online (AJOL)

    Anti-Inflammatory and Antioxidant Activities of Methanol Extracts and Alkaloid Fractions of four Mexican Medicinal Plants of Solanaceae. ... equivalents/g of extract), the best antioxidant activity (94.80% inhibition of DPPH and 97.57% of ABTS) and the highest anti-inflammatory activity (81.93% inhibition of the inflammation).

  18. Evaluation of antibacterial, antitumor, antioxidant activities and ...

    African Journals Online (AJOL)

    Results: Generally, yellow loosestrife extracts demonstrated antibacterial activity against Gram-positive bacteria (Staphylococcus aureus, S. epidermidis and Streptococcus pyogenes). Strong antitumor activity of yellow loosestrife was observed via potato disc diffusion bioassay. Nine different phenolics were also determined ...

  19. In vitro antioxidant activity of Rubus ellipticus fruits

    OpenAIRE

    Sharma, Uma Shankar; Kumar, Arun

    2011-01-01

    Various studies have been done to identify antioxidants from plant sources and efforts have been taken to incorporate it in conventional therapy. In our present study, petroleum ether, ethanolic, and aqueous extracts of Rubus ellipticus fruits have been evaluated for in vitro antioxidant activity using DPPH radical scavenging and reducing power assay. BHA was used as a standard antioxidant for DPPH radical scavenging activity. The reducing power assay of extracts was carried out with ascorbic...

  20. Cytotoxic and antioxidant activity of selected marine sponges

    Directory of Open Access Journals (Sweden)

    Chairman K

    2012-06-01

    Full Text Available Objective: To evaluate the anticancer activity of the crude extracts of Rhabdastrella globostellata (R. globostellata and Spirastrella inconstans (S. inconstans var. moeandrina Dendy. Methods: Soxhlet extraction method was used to extract the secondary metabolites and various assays antioxidant, anticancer and various assays were carried out. The extract were tested anticancer activity against a HeLa, Raw 264.7 and Hek-293. Results: The sponge extracts tested exhibited from median to high toxicity in at least one of the toxicity bioassays performed. The antioxidant activity of the isolated metabolite in ethylacetate solution was assessed by SOD and GTH assays and compared with that of other known natural antioxidants. Conclusions: Potent antioxidants have been detected among both phenolic metabolites and alkaloids. Antioxidant effects of tested compounds have been attributed to their action as chain-breaking antioxidants and/or as scavengers of radicals

  1. Evaluation of the Antioxidant Activity of Syzygium cumini Leaves

    Directory of Open Access Journals (Sweden)

    Yi Ming Lin

    2008-10-01

    Full Text Available The antioxidant activity of Syzygium cumini leaf extracts was investigated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH free radical-scavenging and ferric-reducing antioxidant power (FRAP assays. The methanolic extract and its four water, ethyl acetate, chloroform, and n-hexane fractions were prepared and subjected to antioxidant evaluation. The results showed that the ethyl acetate fraction had stronger antioxidant activity than the other ones. HPLC data indicated that S. cumini leaf extracts contained phenolic compounds, such as ferulic acid and catechin, responsible for their antioxidant activity. A significant linear relationship between antioxidant potency, free radical-scavenging ability and the content of phenolic compounds of leaf extracts supported this observation.

  2. Acetylcholinesterase Inhibition and in Vitro and in Vivo Antioxidant Activities of Ganoderma lucidum Grown on Germinated Brown Rice

    Directory of Open Access Journals (Sweden)

    Beong Ou Lim

    2013-06-01

    Full Text Available In this study, the acetylcholinesterase inhibition and in vitro and in vivo antioxidant activities of Ganoderma lucidum grown on germinated brown rice (GLBR were evaluated. In antioxidant assays in vitro, GLBR was found to have strong metal chelating activity, DPPH, ABTS, hydroxyl and superoxide radical scavenging activity. Cell-based antioxidant methods were used, including lipid peroxidation on brain homogenate and AAPH-induced erythrocyte haemolysis. In antioxidant assays in vivo, mice were administered with GLBR and this significantly enhanced the activities of antioxidant enzymes in the mice sera, livers and brains. The amount of total phenolic and flavonoid compounds were 43.14 mg GAE/g and 13.36 mg CE/g dry mass, respectively. GLBR also exhibited acetylcholinesterase inhibitory activity. In addition, HPLC analyses of GLBR extract revealed the presence of different phenolic compounds. These findings demonstrate the remarkable potential of GLBR extract as valuable source of antioxidants which exhibit interesting acetylcholinesterase inhibitory activity.

  3. Acetylcholinesterase inhibition and in vitro and in vivo antioxidant activities of Ganoderma lucidum grown on germinated brown rice.

    Science.gov (United States)

    Hasnat, Abul; Pervin, Mehnaz; Lim, Beong Ou

    2013-06-07

    In this study, the acetylcholinesterase inhibition and in vitro and in vivo antioxidant activities of Ganoderma lucidum grown on germinated brown rice (GLBR) were evaluated. In antioxidant assays in vitro, GLBR was found to have strong metal chelating activity, DPPH, ABTS, hydroxyl and superoxide radical scavenging activity. Cell-based antioxidant methods were used, including lipid peroxidation on brain homogenate and AAPH-induced erythrocyte haemolysis. In antioxidant assays in vivo, mice were administered with GLBR and this significantly enhanced the activities of antioxidant enzymes in the mice sera, livers and brains. The amount of total phenolic and flavonoid compounds were 43.14 mg GAE/g and 13.36 mg CE/g dry mass, respectively. GLBR also exhibited acetylcholinesterase inhibitory activity. In addition, HPLC analyses of GLBR extract revealed the presence of different phenolic compounds. These findings demonstrate the remarkable potential of GLBR extract as valuable source of antioxidants which exhibit interesting acetylcholinesterase inhibitory activity.

  4. Total Phenolic Content and Antioxidant Activity of Some Malvaceae Family Species

    Directory of Open Access Journals (Sweden)

    Adriana Maria Fernandes de Oliveira

    2012-10-01

    Full Text Available The antioxidant activity of four species of the Malvaceae family (Sidastrum micranthum (A. St.-Hil. Fryxell, Wissadula periplocifolia (L. C. Presl, Sida rhombifolia (L. E. H. L and Herissantia crispa L. (Brizicky were studied using the total phenolic content, DPPH radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC assays. The antioxidant activity of the crude extract, phases and two isolated flavonoids, kaempferol 3,7-di-O-α-l-rhamnopyranoside (lespedin and kaempferol 3-O-β-d-(6''-E-p-coumaroil glucopyranoside (tiliroside was determined. The results showed that there is a strong correlation between total polyphenol contents and antioxidant activity of the crude extract of Sidastrum micranthum and Wissadula periplocifolia; however, this was not observed between Sida rhombifolia and Herissantia crispa. The ethyl acetate (EaF phase showed the best antioxidant effect in the total phenolics, DPPH and TEAC assays, followed by the chloroform (CfF phase, in most species tested. Lespedin, isolated from the EaF phase of W. periplocifolia and H. crispa may not be responsible for the antioxidant activity due to its low antioxidant activity (IC50: DPPH: 1,019.92 ± 68.99 mg/mL; TEAC: 52.70 ± 0.47 mg/mL; whereas tiliroside, isolated from W. periplocifolia, H. crispa and S. micrantum presented a low IC50 value (1.63 ± 0.86 mg/mL compared to ascorbic acid in the TEAC assay.

  5. Total Phenolic Content and Antioxidant Activity of Some Malvaceae Family Species.

    Science.gov (United States)

    Fernandes de Oliveira, Adriana Maria; Sousa Pinheiro, Lilian; Souto Pereira, Charlane Kelly; Neves Matias, Wemerson; Albuquerque Gomes, Roosevelt; Souza Chaves, Otemberg; Vanderlei de Souza, Maria de Fátima; Nóbrega de Almeida, Reinaldo; Simões de Assis, Temilce

    2012-10-26

    The antioxidant activity of four species of the Malvaceae family (Sidastrum micranthum (A. St.-Hil.) Fryxell, Wissadula periplocifolia (L.) C. Presl, Sida rhombifolia (L.) E. H. L and Herissantia crispa L. (Brizicky)) were studied using the total phenolic content, DPPH radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC) assays. The antioxidant activity of the crude extract, phases and two isolated flavonoids, kaempferol 3,7-di-O-α-l-rhamnopyranoside (lespedin) and kaempferol 3-O-β-d-(6''-E-p-coumaroil) glucopyranoside (tiliroside) was determined. The results showed that there is a strong correlation between total polyphenol contents and antioxidant activity of the crude extract of Sidastrum micranthum and Wissadula periplocifolia; however, this was not observed between Sida rhombifolia and Herissantia crispa. The ethyl acetate (EaF) phase showed the best antioxidant effect in the total phenolics, DPPH and TEAC assays, followed by the chloroform (CfF) phase, in most species tested. Lespedin, isolated from the EaF phase of W. periplocifolia and H. crispa may not be responsible for the antioxidant activity due to its low antioxidant activity (IC50: DPPH: 1,019.92 ± 68.99 mg/mL; TEAC: 52.70 ± 0.47 mg/mL); whereas tiliroside, isolated from W. periplocifolia, H. crispa and S. micrantum presented a low IC50 value (1.63 ± 0.86 mg/mL) compared to ascorbic acid in the TEAC assay.

  6. QSAR models for antioxidant activity of new coumarin derivatives.

    Science.gov (United States)

    Erzincan, P; Saçan, M T; Yüce-Dursun, B; Danış, Ö; Demir, S; Erdem, S S; Ogan, A

    2015-01-01

    This study presents 37 new antioxidant coumarin derivatives and strategies for structural modification to improve their antioxidant activities, the main ferric-reducing antioxidant power (FRAP) assay used to evaluate their antioxidant properties and the generation of validated quantitative structure-activity (antioxidant activity) relationship (QSAR) models. In an attempt to generate QSAR models, structures of all coumarin derivatives in the data set were fully optimized by semi-empirical PM6 method using SPARTAN 10 software. Descriptors were calculated by DRAGON 6.0 software. Multiple linear regression (MLR) models were developed with different training/test set combinations using QSARINS 2.2.1 software. Robustness, reliability and predictive power of the models were tested by internal and external validations. Applicability domain of the best two-descriptor model (nTR = 30; r(2) = 0.924; RMSETR = 0.213; nTEST = 7; r(2)ext = 0.887; RMSEext = 0.255; CCCext = 0.939) was determined. Descriptors appeared in the model revealed that complexity, H-bond donor and lipophilic character are important parameters in describing the antioxidant activity. Apart from the compounds in the data set, we also designed 31 new antioxidant coumarin derivatives and predicted their antioxidant activity using the best two-descriptor model. Most of these compounds are promising antioxidants.

  7. Phytochemical Contents and Antioxidant and Antiproliferative Activities of Selected Black and White Sesame Seeds

    Science.gov (United States)

    Abbasi, Arshad Mehmood; Zheng, Bisheng

    2016-01-01

    Sesame (Sesamum indicum L.) seeds are popular nutritional food but with limited knowledge about their antioxidant and antiproliferative activities of various varieties. Phytochemical profiles and antioxidant and antiproliferative activities of six varieties of sesame (Sesamum indicum L.) seeds were studied. Fenheizhi3 (black) cultivar exhibited the maximum contents of total phenolics and lignans and values of total oxygen radical absorbance capacity (ORAC) and antiproliferative activity (EC50) against HepG2 cells. Bound ORAC values showed strong associations with bound phenolics contents (r = 0.976, p 0.8, p < 0.05). Interestingly, nonlignan components in bound phenolics contributed to the antioxidant and antiproliferative activities. This study suggested that Fenheizhi3 variety is superior to the other five varieties as antioxidant supplements. PMID:27597975

  8. The antioxidant activity of wild medlar ( Mespilus germanica L.) fruit ...

    African Journals Online (AJOL)

    The medlar is an edible fruit. Modern medicine has recognized its healing properties in the treatment of some diseases. There is no scientific data in the literature about the antioxidant activity of methanol or aqueous extract of medlar fruit, leaf or stem bark. Antioxidant activities of these parts were evaluated by employing six ...

  9. Comparative Study of Antioxidant Activities of the Leaves and Stem ...

    African Journals Online (AJOL)

    Leaves and stem extracts of Ipomoea aquatic Forsk were analysed for their antioxidant activities. Folin-Ciocalteu and AlCl3 methods were used to quantify total phenolic and flavonoid contents while 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical assay was used to examine the antioxidant activity of the extracts.

  10. Phenolic Content, and Antioxidant and Antimicrobial Activities of ...

    African Journals Online (AJOL)

    Erah

    Methods: The content of total phenols, flavonoids and anthocyanins of the alcohol, hydroalcohol and aqueous extracts of ... Keywords: Crataegus oxyacantha L.; Natural phenolic compounds; Antioxidant and antimicrobial activity, Southeast Serbia. ..... Antioxidant activities of Sechium edule (Jacq.) Swart extracts, Food ...

  11. In Vitro Antioxidant Activity of Chitosan Aqueous Solution: Effect of ...

    African Journals Online (AJOL)

    Purpose: To investigate the effect of salt form on the antioxidant activities of chitosan aqueous solution. Methods: The antioxidant activities of chitosan acetate (CS-acetate), chitosan hydroxybenzotriazole (CS-HOBt), chitosan thiamine pyrophosphate (CS-TPP) and chitosan ethylenediaminetetraacetic acid (CS-EDTA) ...

  12. Antioxidant and antibacterial activities of Cladophora glomerata (L ...

    African Journals Online (AJOL)

    Cladophora is one of the largest filamentous green-algal genus and has a widespread distribution in Caspian Sea Coast. This study aimed at assaying the antimicrobial and antioxidant activities of Cladophora glomerata in South of Caspian sea. The antioxidant activity of the extract was investigated, including the total ...

  13. Response of total phenolic content and antioxidant activities of bush ...

    African Journals Online (AJOL)

    The positive health benefits associated with tea are made possible by the antioxidant activity of phenolic compounds present in tea. The total phenolic content and antioxidant activity of bush tea (Athrixia phylicoides DC.) and special tea (Monsonia burkeana) were studied. The extractions were done in triplicate using cold ...

  14. High Levels of Phytophenolics and Antioxidant Activities in Oryza ...

    African Journals Online (AJOL)

    Purpose: To investigate phenolic levels, phytophenolic profiles and total antioxidant activities of Oryza sativa, unpolished Thai rice. Methods: Unpolished Thai rice strains of Leum Phua, Klam, Hawm Nil and Black Rose were measured for antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ...

  15. Antioxidant, hypoglycemic and anti-diabetic activities of Ziziphus ...

    African Journals Online (AJOL)

    Antioxidant, hypoglycemic and anti-diabetic activities of Ziziphus spina-christi (L) Willd (Rhamnacae) leaf extract. Amal AM Al-Ghamdi, Abdelaaty A Shahat. Abstract. Purpose: To investigate the antioxidant, hypoglycemic and antidiabetic activities as well as the phenolic composition of Ziziphus spina-christi (ZSC) Methods: ...

  16. Comparative Antioxidant Activity of Hibiscus sabdariffa and Ascorbic ...

    African Journals Online (AJOL)

    Nx 6110

    Lipid peroxidation is becoming a popular biological marker of oxidative stress. Hibiscus sabdariffa has been reported to serve as a herbal remedy for various disease conditions, but studies on its antioxidant activity and the extent to which it acts remain scarce. The antioxidant activity of H. sabdariffa aqueous extracts, an.

  17. Antimicrobial And Antioxidant Activities Of Some Nigerian Medicinal ...

    African Journals Online (AJOL)

    Ten Nigerian plants suggested from their ethnomedical uses to possess antimicrobial and antioxidant activities were studied for their anti-microbial and anti-oxidant properties. Antimicrobial activity was tested against Escherichia coli NCTC 10418, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, ...

  18. Extraction and Antioxidant Activity of Phenolic Compounds from ...

    African Journals Online (AJOL)

    1School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, 2Department of Food Science, Xinyang. College of Agriculture ... Purpose: To investigate the extraction and antioxidant activity of phenolic compounds from Okra flowers. ..... antioxidant activities of essential oil and methanol extract of ...

  19. Comparative Antioxidant Activity of Hibiscus sabdariffa and Ascorbic ...

    African Journals Online (AJOL)

    Lipid peroxidation is becoming a popular biological marker of oxidative stress. Hibiscus sabdariffa has been reported to serve as a herbal remedy for various disease conditions, but studies on its antioxidant activity and the extent to which it acts remain scarce. The antioxidant activity of H. sabdariffa aqueous extracts, ...

  20. Antioxidant Activities of Methanol Extract and Solvent Fractions of ...

    African Journals Online (AJOL)

    Purpose: To determine the antioxidant activity of methanol extract (ME) and solvent fractions of Avrainvillea erecta as well as their total phenolic and flavonoid contents. Methods: The antioxidant activities of ME as well as its chloroform, butanol, and aqueous fractions (CF, BF and WF, respectively) of A. erecta were ...

  1. Antioxidant activities of solvent extracts from endemic Cyclamen ...

    African Journals Online (AJOL)

    Leaves extracts of CM exhibited higher antioxidant activity than tuber extracts with all the types of solvent used. All concentrations of petroleum ether, acetone, methanol and water extracts of CM leaves showed higher antioxidant activities than that of 0.5 mg of á-tocopherol (42%) and close to BHT (99.30%) and had 96.60, ...

  2. Application of KRL test to assess total antioxidant activity in pigs: sensitivity to dietary antioxidants.

    Science.gov (United States)

    Rossi, Raffaella; Pastorelli, Grazia; Corino, Carlo

    2013-04-01

    The application of Kit Radicaux Libres (KRL) test to assess total blood antioxidant activity in pigs was evaluated. The KRL has been validated and is widely used in humans for assessing the effectiveness of natural or pharmaceutical treatments, and in vitro to evaluate the antioxidant activities of natural or synthetic antioxidants. In this study the sensitivity of the KRL test in assessing the effectiveness of dietary antioxidant supplementation (vitamin E and plant extract) was evaluated in two different phases of pig breeding. The first trial, in post-weaned piglets (40 piglets/group) fed dietary vitamin E supplementation for 60 days, indicated that there was a higher total antioxidant activity (P=0.032) of whole blood and of red blood cells (P=0.001) than for control pigs. The second trial indicated that long-term supplementation of water soluble plant extract (20 pigs/group) from the leaves of Verbenaceae (Lippia spp.) tended (P=0.091) to increase antioxidant activity in the whole blood of treated, rather than control pigs. These results indicate that the KRL might be recommended as one of efficient means for evaluating antioxidant activity of dietary ingredients fed to pigs. Copyright © 2012 Elsevier Ltd. All rights reserved.

  3. Antioxidative activity of Silibum marianum cultivated in Mongolia

    Directory of Open Access Journals (Sweden)

    J Ganbaatar

    2014-12-01

    Full Text Available Silibum marianum (Milk thistle contains high amount of phenolic compounds with antioxidant properties. The aim of this work was to evaluate the antioxidant activity of  phenolic compounds included in Milk thistle. The  antioxidant properties of the leaves and seeds of milk thistle were examined by determining its ability to scavenge the 2,2-diphenyl-1-picrylhydrazyl (DPPH free radical. The results obtained from DPPH assay confirm that Milk thistle extracts have high antioxidative activity.DOI: http://doi.dx.org/10.5564/mjc.v15i0.323 Mongolian Journal of Chemistry 15 (41, 2014, p53-55

  4. Activity of Strongly Magnetized Neutron Stars

    Science.gov (United States)

    Beloborodov, Andrei

    This proposal is the continuation of a previous 3-year project that focused on modeling the nonthermal emission from magnetars and pulsars and testing the models against new observations, in particular by NuSTAR. The proposed project develops in two directions: (1) First-principle simulations of the magnetospheric electron-positron discharge using our code APERTURE (based on the particle-in-cell method), which is specifically designed for this purpose. Its performance is demonstrated by the first application to rotation-powered pulsars, and it can significantly advance our understanding of the magnetospheric activity of magnetars and pulsars. Our simulations involve a detailed implementation of radiative processes, tracking the emission and propagation of gammarays and production of electron-positron pairs. The results will provide new theoretical foundation for interpreting emission from the twisted magnetospheres of neutron stars. They will clarify, in particular, the radiative mechanism of magnetar bursts and persistent emission. (2) Investigation of magnetic field evolution inside neutron stars, which is ultimately responsible for driving the magnetospheric activity of magnetars and their surface heating. Our recent results suggest two novel phenomena in the solid crust of an active magnetar: thermoplastic waves and Hall-mediated avalanches. We propose to investigate scenarios for the global magnetic field evolution in the core and the crust, and its observables including (a) twisting of the external magnetosphere and the resulting nonthermal activity, (b) subsurface heating, and (c) sudden changes of the rotation rate. We will use our models and the rich accumulated data to disentangle the key dynamic processes inside magnetars. This analysis can constrain the magnetic fields hidden inside magnetars, the state of their core matter and its possible superfluidity.

  5. Antioxidant activity, phenolic content and colour of the Slovak cabernet sauvignon wines

    Directory of Open Access Journals (Sweden)

    Daniel Bajčan

    2016-01-01

    Full Text Available Antioxidants are specific substances that oxidize themselves and in this way they protect other sensitive bioactive food components against destruction. At the same time, they restrict the activity of free radicals and change them to less active forms. Grapes and wine are a significant source of antioxidants in human nutrition. One of the most important group occuring in grapes and wines are polyphenols. Many of phenolic compounds have been reported to have multiple biological activities, including cardioprotective, anti-inflammatory, anti-carcinogenic, antiviral and antibacterial properties attributed mainly to their antioxidant and antiradical activity. Therefore, it is important to know the content of polyphenols and their antioxidant effects in foods and beverages. Twenty-eight Cabernet Sauvignon wine samples, originated from different Slovak vineyard regions, were analyzed using spectrophotometry for the content of total polyphenols, content of total anthocyanins, antioxidant activity and wine colour density. Determined values of antioxidant activity in observed wines were within the interval 69.0 - 84.2% inhibition of DPPH (average value was 78.8% inhibition of DPPH and total polyphenol content ranged from 1,218 to 3,444 mg gallic acid per liter (average content was 2,424 mg gallic acid.L-1. Determined total anthocyanin contents were from 68.6 to 430.7 mg.L-1 (average content was 220.6 mg.L-1 and values of wine colour density ranged from 0.756 to 2.782 (average value was 1.399. The statistical evaluation of the obtained results did not confirm any linear correlations between total polyphenol content, resp. total anthocyanin content and antioxidant activity. The correlations between total polyphenol content and total anthocyanin content, resp. the content of total anthocyanins and wine colour density were strong. The results confirmed very strong correlations between wine colour density and total polyphenol content, resp. antioxidant

  6. Antioxidant activity and polyphenolic content of Phragmites karka under saline conditions

    International Nuclear Information System (INIS)

    Abideen, Z.; Qasim, M.; Rasheed, A.; Adnan, M.Y.; Khan, A.

    2015-01-01

    Phragmites karka (Retz.) Trin. ex Steud. is a halophytic grass found in inland saline marshes potentially useful for biofuel industry. Growth, polyphenol content and antioxidant activity in different plant parts were investigated after 45 days NaCl (0,100, 200 and 400 mM) treatments. An increased in polyphenolic content (5.06 to 12.81 mg GAE g-1 DW) and antioxidant activity (0.64-3.21 IC50 mg ml-1 for DPPH and 9.09-17.91 mM Fe+2 g-1 DW for FRAP) was observed with the increasing salt concentrations among different plant parts. Increase in plant biomass, phenolic content and antioxidant activity with lower MDA at 100 mM NaCl indicates a strong protection against oxidative damage. Leaves exhibited highest polyphenol and antioxidant activity, followed by stem and root. Coefficient of regression shows the high predictability of antioxidant activity (0.705) and phenolic contents (0.763) with an increase in salinity. Our data indicates a link between production of polyphenolic antioxidants and salt stress in P. karka which indicates salinity as an effective tool to produce antioxidant rich biomass for industrial purposes. (author)

  7. Antioxidant activities of protein hydrolysates obtained from the housefly larvae.

    Science.gov (United States)

    Zhang, Huan; Wang, Pan; Zhang, Ai-Jun; Li, Xuan; Zhang, Ji-Hong; Qin, Qi-Lian; Wu, Yi-Jun

    2016-09-01

    The housefly is an important resource insect and the housefly larvae are ideal source of food additives. The housefly larvae protein hydrolysates were obtained by enzymatic hydrolysis by alcalase and neutral proteinase. Their antioxidant activities were investigated, including the superoxide and hydroxyl radicalscavenging activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, reducing power and metal chelating activity. The antioxidant activities of both hydrolysates increased with their increasing concentrations. The alcalase hydrolysate (AH) showed higher scavenging activities against hydroxyl radical and superoxide anion radical at low concentrations and higher metal-chelating activity than the neutral proteinase hydrolysate (NPH). The NPH exhibited higher scavenging activity against DPPH free radical and higher reducing power than the AH. Both hydrolysates showed more than 50% superoxide anion radical-scavenging activity at 10 μg/mL. These results indicate that both housefly larvae protein hydrolysates display high antioxidant activities and they could serve as potential natural antioxidant food additives.

  8. Erythrocyte membrane stabilization effect and antioxidant activity of methyl methacrylate

    International Nuclear Information System (INIS)

    Popov, B.

    2004-01-01

    Methyl methacrylate (MMK) is a synthetic product with mild impact on human health that is not well studied on cellular basis. Here, human erythrocytes were used to investigate the effects MMK exerts on acid and heat-induced hemolysis. Biphasic effect of MMK was observed for acid-induced hemolysis; i.e., protection at low (0 - 0.05% v/v) and stimulation at higher (0.1- 0.4% v/v) concentrations. The maximal protective effect was produced at 0.03% (v/v). At this concentration MMK increased the temperatures of heat denaturation of erythrocyte membrane proteins, spectrin and integral proteins, by about 2 0 C and inhibited the heat-induced hemolysis by 20 %. This membrane stabilization effect of MMK is similar to that produced by some anti-inflammatory and antirheumatic drugs. The increased acid resistance possibly indicated anti-oxidant properties of MMK. The nonenzymatic antioxidant activity test evidenced that MMK has no superoxide dismutase-like activity but demonstrates strong catalase-like activity (about 900 kU/mmol at 0.05-0.1 mmol/l concentration). The results indicate that at low concentration MMK exerts benign effect on cellular membrane that could find therapeutic usage. (author)

  9. Antioxidant activity and calcium binding of isomeric hydroxybenzoates

    Directory of Open Access Journals (Sweden)

    Zichen Zhao

    2018-04-01

    Full Text Available The association constant for calcium binding to hydroxybenzoates in aqueous 0.16 M NaCl at 25 °C was found electrochemically to have the value Kass = 280 mol L−1 with ΔHo = −51 kJ mol−1, ΔSo = −122 J mol−1 K−1 for the 2-isomer (salicylate, Kass = 7 mol L−1 with ΔHo = −39 kJ mol−1, ΔSo = −116 J mol−1 K−1 for the 3-isomer, and Kass = 8 mol L−1 with ΔHo = −51 kJ mol−1, ΔSo = −155 J mol−1 K−1 for the 4-isomer. The 3- and 4-isomers were found more efficient as antioxidants than the 2-isomer in decreasing oxygen consumption rate in a peroxidating methyl linoleate emulsion and less sensitive to presence of calcium. All isomers were found prooxidative for iron-catalyzed initiation of oxidation due to enhanced radical formation as shown by electron spin resonance spectroscopy. Calcium salicylate was found to have low solubility with a solubility product Ksp = 4.49·10−6 based on activity with ΔHo = 67 kJ mol−1, ΔSo = 123 J mol−1 K−1 for dissolution in water, when corrected for the strong complex formation. Calcium in food and beverages may thus lower antioxidant activity of plant phenols through complexation or by precipitation. Keywords: Antioxidant activity, Calcium binding, 2-Hydroxybenzoate, 3-Hydroxybenzoate, 4-Hydroxybenzoate

  10. Study on the antioxidant activity and membrane interaction of a multiple antioxidant

    DEFF Research Database (Denmark)

    Jodko-Jodko-Piórecka, Kasia; Klösgen, Beate; Litwinienko, Grzegorz

    Catecholamines, including dopamine, have a role in the transduction of nervous stimuli. Apart from that, they might behave as endogenous phenolic antioxidants protecting the neuronal tissue from deleterious effects of oxidative stress. Results from our preliminary study1 even indicate a synergistic...... effect: an interplay of catecholamines with other molecules seems to enhance their antioxidant activity (e.g. 3,4-dihydroxyphenylalanine - L-DOPA -, and 2,2,5,7,8-pentamethyl-6-hydroxychroman - PMHC -, an analogue of α-tocopherol). Addition of small amounts of catecholamines suppresses or largely...... inhibits the peroxidation of lipids2. The understanding of the mechanism of antioxidant action of catecholamines and their interplay with other antioxidants in lipid membranes requires interdisciplinary research on the kinetics and thermodynamics of antioxidant(s)/membrane interactions. Here we report...

  11. Thai Fruits Exhibit Antioxidant Activity and Induction of Antioxidant Enzymes in HEK-293 Cells.

    Science.gov (United States)

    Anantachoke, Natthinee; Lomarat, Pattamapan; Praserttirachai, Wasin; Khammanit, Ruksinee; Mangmool, Supachoke

    2016-01-01

    The cellular antioxidant enzymes play the important role of protecting the cells and organisms from the oxidative damage. Natural antioxidants contained in fruits have attracted considerable interest because of their presumed safety and potential nutritional value. Even though antioxidant activities of many fruits have been reported, the effects of phytochemicals contained in fruits on the induction of antioxidant enzymes in the cells have not been fully defined. In this study, we showed that extracts from Antidesma ghaesembilla , Averrhoa bilimbi , Malpighia glabra , Mangifera indica, Sandoricum koetjape , Syzygium malaccense, and Ziziphus jujuba inhibited H 2 O 2 -induced intracellular reactive oxygen species production in HEK-293 cells. Additionally, these Thai fruit extracts increased the mRNA and protein expressions of antioxidant enzymes, catalase, glutathione peroxidase-1, and manganese superoxide dismutase. The consumption of Thai fruits rich in phenolic compounds may reduce the risk of oxidative stress.

  12. Thai Fruits Exhibit Antioxidant Activity and Induction of Antioxidant Enzymes in HEK-293 Cells

    Directory of Open Access Journals (Sweden)

    Natthinee Anantachoke

    2016-01-01

    Full Text Available The cellular antioxidant enzymes play the important role of protecting the cells and organisms from the oxidative damage. Natural antioxidants contained in fruits have attracted considerable interest because of their presumed safety and potential nutritional value. Even though antioxidant activities of many fruits have been reported, the effects of phytochemicals contained in fruits on the induction of antioxidant enzymes in the cells have not been fully defined. In this study, we showed that extracts from Antidesma ghaesembilla, Averrhoa bilimbi, Malpighia glabra, Mangifera indica, Sandoricum koetjape, Syzygium malaccense, and Ziziphus jujuba inhibited H2O2-induced intracellular reactive oxygen species production in HEK-293 cells. Additionally, these Thai fruit extracts increased the mRNA and protein expressions of antioxidant enzymes, catalase, glutathione peroxidase-1, and manganese superoxide dismutase. The consumption of Thai fruits rich in phenolic compounds may reduce the risk of oxidative stress.

  13. Phytochemical constituents and antioxidant activities of aqueous ...

    African Journals Online (AJOL)

    USER

    2010-08-02

    Aug 2, 2010 ... 0.05) than methanol extract (77.00 ± 0.84%). Nevertheless, methanol and aqueous extracts of C. afer possess anti-oxidative properties as well as bioactive metabolites. Thus, stem extracts of C. afer could serve as sources of antioxidants and bioactive compounds for nutrition and therapeutic purposes.

  14. Physiochemical composition and antioxidant activities of ...

    African Journals Online (AJOL)

    USER

    2010-07-12

    Jul 12, 2010 ... Dietary fiber consumption regardless of soluble as well as insoluble fibers was reported to have positive effects in lowering the risk of cardiovascular disease, gastrointestinal disease, colon cancer and obesity. (Rosamond, 2002). Antioxidant properties of MPP and MPJP. Antioxidant constituents.

  15. Relationship between Estradiol and Antioxidant Enzymes Activity of Ischemic Stroke

    Directory of Open Access Journals (Sweden)

    Nasrin Sheikh

    2009-01-01

    Full Text Available Some evidence suggests the neuroprotection of estrogen provided by the antioxidant activity of this compound. The main objective of this study was to determine the level of estradiol and its correlation with the activity of antioxidant enzymes, total antioxidant status and ferritin from ischemic stroke subjects. The study population consisted of 30 patients with acute ischemic stroke and 30 controls. There was no significant difference between estradiol in stroke and control group. The activity of superoxide dismutase and level of ferritin was higher in stroke compared with control group (<.05, <.001, resp.. There was no significant correlation between estradiol and glutathione peroxidase, glutathione reductase, catalase, total antioxidant status, and ferritin in stroke and control groups. We observed inverse correlation between estradiol with superoxide dismutase in males of stroke patients (=−0.54, =.029. Our results supported that endogenous estradiol of elderly men and women of stroke or control group has no antioxidant activity.

  16. Study of Stevia rebaudiana Bertoni antioxidant activities and cellular properties.

    Science.gov (United States)

    Bender, Cecilia; Graziano, Sara; Zimmermann, Benno F

    2015-01-01

    The aim of our study was to determine the antioxidant activities, cytotoxicity and proliferative properties in Stevia rebaudiana leaves and stems. Leaves extracts exhibited a higher antioxidant activity than stems extract, through oxygen radical absorbance capacity (ORAC) and cellular antioxidant activity (CAA) assays. Stevioside and rebaudioside A, the main sweetening metabolites in stevia leaves, exhibited a low ORAC value in comparison with plant extracts, while did not elicit any CAA. Stevia rebaudiana did not exhibit toxicity against HepG2 (hepatocellular carcinoma) human cells. No proliferative nor catalase modulations were observed in cells treated with such extracts. Our findings support the promising role of stevia that, apart from its sweetness, can act as a source of antioxidants, even at the intracellular level. This activity makes S. rebaudiana crude extract an interesting resource of natural sweetness with antioxidant properties which may find numerous applications in foods and nutritional supplements industries.

  17. Interactions between Carotenoids from Marine Bacteria and Other Micronutrients: Impact on Stability and Antioxidant Activity.

    Science.gov (United States)

    Sy, Charlotte; Dangles, Olivier; Borel, Patrick; Caris-Veyrat, Catherine

    2015-11-19

    Recently isolated spore-forming pigmented marine bacteria Bacillus indicus HU36 are sources of oxygenated carotenoids with original structures (about fifteen distinct yellow and orange pigments with acylated d-glucosyl groups). In this study, we evaluated the stability (sensitivity to iron-induced autoxidation) and antioxidant activity (inhibition of iron-induced lipid peroxidation) of combinations of bacterial HU36 carotenoids with the bacterial vitamin menaquinone MQ-7 and with phenolic antioxidants (vitamin E, chlorogenic acid, rutin). Unexpectedly, MQ-7 strongly improves the ability of HU36 carotenoids to inhibit Fe(II)-induced lipid peroxidation, although MQ-7 was not consumed in the medium. We propose that their interaction modifies the carotenoid antioxidant mechanism(s), possibly by allowing carotenoids to scavenge the initiating radicals. For comparison, β-carotene and lycopene in combination were shown to exhibit a slightly higher stability toward iron-induced autoxidation, as well as an additive antioxidant activity as compared to the carotenoids, individually. HU36 carotenoids and phenolic antioxidants displayed synergistic activities in the inhibition of linoleic acid peroxidation induced by heme iron, but not by free iron. Synergism could arise from antioxidants interacting via electron transfer through the porphyrin nucleus of heme iron. Overall, combining antioxidants acting via complementary mechanisms could be the key for optimizing the activity of this bacterial carotenoid cocktail.

  18. Antioxidant activity of wine assessed by different in vitro methods

    Directory of Open Access Journals (Sweden)

    Di Lorenzo Chiara

    2017-01-01

    Full Text Available Epidemiological studies have suggested that a diet rich in antioxidant compounds could help in counteracting the effects of reactive oxygen species, reducing the risk factors for chronic diseases. The moderate consumption of wine, especially red wine, has been associated with the reduction in mortalities from cardiovascular diseases. One of the possible reasons for the protective effect of wine can be identified in the high content of polyphenols (mainly flavonoids, which have significant antioxidant activity. Even though several in vitro tests have been developed for the measure of the antioxidant property, no method has showed a satisfactory correlation with the in vivo situation. On these bases, the aim of this study was the application and comparison of different in vitro methods to assess the antioxidant activity of red, rosé and white wines. The methods were: 1 Folin-Cocalteau's assay for the quantification of total polyphenol content; 2 the DPPH (1,1-diphenyl-2-picrylhydrazyl spectrophotometric assay and the Trolox Equivalent Antioxidant Capacity (TEAC spectrophotometric assay for measuring the antioxidant activity of samples; 3 High Performance Thin Layer Chromatography for separation of phenolic substances and assessment of the associated antioxidant activity; 4 electrochemical detection by using a biosensor. Although all the approaches show some limitations, this battery of tests offers a more reliable body of data on the antioxidant activity of vine derivatives.

  19. Reviews on Mechanisms of In Vitro Antioxidant Activity of Polysaccharides

    Science.gov (United States)

    Wang, Junqiao; Hu, Shuzhen; Nie, Shaoping; Yu, Qiang; Xie, Mingyong

    2016-01-01

    It is widely acknowledged that the excessive reactive oxygen species (ROS) or reactive nitrogen species (RNS) induced oxidative stress will cause significant damage to cell structure and biomolecular function, directly or indirectly leading to a number of diseases. The overproduction of ROS/RNS will be balanced by nonenzymatic antioxidants and antioxidant enzymes. Polysaccharide or glycoconjugates derived from natural products are of considerable interest from the viewpoint of potent in vivo and in vitro antioxidant activities recently. Particularly, with regard to the in vitro antioxidant systems, polysaccharides are considered as effective free radical scavenger, reducing agent, and ferrous chelator in most of the reports. However, the underlying mechanisms of these antioxidant actions have not been illustrated systematically and sometimes controversial results appeared among various literatures. To address this issue, we summarized the latest discoveries and advancements in the study of antioxidative polysaccharides and gave a detailed description of the possible mechanisms. PMID:26682009

  20. Measurement of Antioxidant Activity Towards Superoxide in Natural Waters.

    Directory of Open Access Journals (Sweden)

    D. Whitney King

    2016-11-01

    Full Text Available Antioxidants are a class of molecules that provide a protective function against reactive oxygen species (ROS in biological systems by out competing physiologically important molecules for ROS oxidation. In natural waters, the reactivity of antioxidants gives an estimate of oxidative stress and may determine the reactivity and distribution of reactive oxidants. We present an analytical method to measure antioxidant activity in natural waters through the competition between ascorbic acid, an antioxidant, and MCLA, a chemiluminescent probe for superoxide. A numerical kinetic model of the analytical method has been developed to optimize analytical performance. Measurements of antioxidant concentrations in pure and seawater are possible with detection limits below 0.1 nM. Surface seawater samples collected at solar noon contained over 0.4 nM of antioxidants and exhibited first-order decay with a half-life of 3-7 minutes, consistent with a reactive species capable of scavenging photochemically produced superoxide.

  1. Antioxidant and cytotoxic activity of mono- and bissalicylic acid derivatives

    Directory of Open Access Journals (Sweden)

    Đurendić Evgenija A.

    2014-01-01

    Full Text Available A simple synthesis of mono- and bis-salicylic acid derivatives 1-10 by the transesterification of methyl salicylate (methyl 2-hydroxybenzoate with 3-oxapentane-1,5-diol, 3,6- dioxaoctane-1,8-diol, 3,6,9-trioxaundecane-1,11-diol, propane-1,2-diol or 1-aminopropan- 2-ol in alkaline conditions is reported. All compounds were tested in vitro on three malignant cell lines (MCF-7, MDA-MB-231, PC-3 and one non-tumor cell line (MRC- 5. Strong cytotoxicity against prostate PC-3 cancer cells expressed compounds 3, 4, 6, 9 and 10, all with the IC50 less than 10 μmol/L, which were 11-27 times higher than the cytotoxicity of antitumor drug doxorubicin. All tested compounds were not toxic against the non-tumor MRC-5 cell line. Antioxidant activity of the synthesized derivatives was also evaluated. Compounds 2, 5 and 8 were better OH radical scavengers than commercial antioxidants BHT and BHA. The synthesized compounds showed satisfactory scavenger activity, which was studied by QSAR modeling. A good correlation between the experimental variables IC50 DPPH and IC50 OH and MTI (molecular topological indices molecular descriptors and CAA (accessible Connolly solvent surface area for the new compounds 1, 3, and 5 was observed.

  2. Antioxidant Activity of Some Plant Extracts Towards Xanthine Oxidase, Lipoxygenase and Tyrosinase

    Directory of Open Access Journals (Sweden)

    Pi-Yu Chen

    2009-08-01

    Full Text Available Natural products have the potential to be developed into new drugs for the treatment of various diseases. The aim of the present study was to screen the antioxidant activities of some common edible fruits, garden plants and medicinal plants indigenous to Taiwan. This was performed by assessing the activities of lipoxygenase, xanthine oxidase and tyrosinase following incubation with extracts from these plants. A further aim was to use HPLC-DAD and tyrosinase to chromatographically identify the antioxidative constituents obtained from an extract exhibiting strong antioxidative properties. The acetone extracts of 27 cultivated plant species from Taiwan were tested for antioxidant activities towards xanthine oxidase, tyrosinase and lipoxygenase using spectrophotometric assays. Koelreuteria henryi, Prunus campanulata, and Rhodiola rosea showed the highest xanthine oxidase inhibitory activities. Camellia sinensis, Rhodiola rosea, and Koelreuteria henryi exhibited good tyrosinase inhibitory activities and potent anti-lipoxygenase activities. As Koelreuteria henryi had notable significant inhibitory activities towards xanthine oxidase, tyrosinase, and lipoxygenase, it was further tested with tyrosinase and HPLC-DAD. The results from this part of the study revealed that the more powerful the antioxidant capability of the extracted component, the greater the decrease in peak height obtained after reacting with tyrosinase. Additional studies are warranted to further characterize the compounds responsible for the antioxidant properties of the examined extracts.

  3. Antioxidant Activity of Natural Products against Aluminium Fluoride ...

    African Journals Online (AJOL)

    Evaluation of antioxidant potential of natural products against Aluminium fluoride (AlF4) induced oxidative stress in albino mice were represented in the present study. Gossypin, Quercetin dehydrate, (-)-Epicatechin gallate, Gallic acid and Suramin sulphate (G-protein inhibitor) were evaluated for antioxidant activity by ...

  4. Cytotoxic and Antioxidant Activities of Antidesma thwaitesianum Müll ...

    African Journals Online (AJOL)

    natural antioxidants; however, no specific report regarding health benefits of Antidesma thwaitesianum fruits has been published to date. Thus, the present study is the first to determine the cytotoxic and antioxidant activities of mamao fruit and fruit waste extracts obtained by different extraction methods using chemical and ...

  5. Determination of secondary metabolites and antioxidant activity of ...

    African Journals Online (AJOL)

    and test samples, respectively. IC50, which is the concentration required to obtain a 50 % antioxidant capacity, was determined from a graph of antioxidant activity (%) against extract concentration (µg /mL). Total phenolic contents. The total phenolic content of the samples was determined by the Folin-Ciocalteu method [13].

  6. Antibacterial and antioxidant activities of the essential oils and ...

    African Journals Online (AJOL)

    The present study deals with the evaluation of the in vitro antioxidant and antibacterial activity of phenolic extracts and essential oils of two medicinal and aromatic plants Zygophyllum album and Myrtus communis by using the 2,2- diphenyl-2-picrylhydrazyl radical, total antioxidant power and agar diffusion methods and ...

  7. Propolis in Kenya: Antioxidative and Radical Scavenging Activity in ...

    African Journals Online (AJOL)

    Dept. of Food Science & Technology

    2013-01-01

    Jan 1, 2013 ... Phenolic compounds in plants have properties; that influence health and performance enhancement in humans [1]. ... These bioflavonoids are the main antioxidants found in plant extracts. [4]. Natural antioxidants ..... A New Chalcone with a Potent Radical Scavenging Activity and. Accompanying Phenolics ...

  8. Antidepressant and antioxidant activities of Artemisia absinthium L ...

    African Journals Online (AJOL)

    Artemisia absinthium (Asteraceae) is widely used in Iranian traditional medicine. Its effects may be correlated with the presence of antioxidant compounds. Methanolic extract of A. absinthium aerial part at flowering stage was screened for antioxidant activities by five complementary test systems. Also, its antidepressant ...

  9. Investigation of the antioxidant activity and quantification of the ...

    African Journals Online (AJOL)

    It is also rich in carotene, niacin, riboflavin, thiamine and vitamin C. Objectives: To determine the amounts of toxic heavy metals, quantify the amounts of vitamins A and E and investigate the antioxidant activities of Carrot. Method: The research investigated the antioxidant properties of carrot on the basis of the radical ...

  10. Antioxidant Activities of the Leaf Extract and Fractions of Cola ...

    African Journals Online (AJOL)

    Cola lepidota (CL) K. Schum (sterculiaceae) used in Nigeria folk medicine as febrifuges, pulmonary problems and cancer related ailments. This study evaluated scientifically the in vitro antioxidant activity of extract and fractions with a view to validate its folkloric usage. In vitro antioxidant properties of extract and fractions ...

  11. Antioxidant activity of the medicinal plant Coleus forskohlii Briq.

    African Journals Online (AJOL)

    user

    2011-03-28

    Mar 28, 2011 ... Antioxidant status of different parts of Coleus forskohlii including roots, stem, leaves and tubers was analyzed. For the enzymatic antioxidant properties, the activities of superoxide dismutase, peroxidase, polyphenol oxidase and catalase were significantly higher (P < 0.05) in tubers than in the leaves, roots.

  12. Antimicrobial and antioxidant activities of two endemic plants from ...

    African Journals Online (AJOL)

    In addition to the antioxidant activity of these plants, the total phenolic compounds and flavonoids were also measured in the extracts. ... that the extracts of A. scabriflorum and A. tchihatschewii possess antimicrobial and antioxidant properties, and therefore, they can be used as a natural preservative ingredient in food

  13. Antioxidant and free radical scavenging activities of plant extracts ...

    African Journals Online (AJOL)

    Twenty-two species of medicinal plants collected in the Mexican state of Morelos were selected to evaluate their free radical scavenging and antioxidant activities. The extracts from the aerial parts of the plants were obtained using hexane, acetone and methanol (66 extracts). The initial qualitative screening of antioxidants ...

  14. Total Antioxidant Activity, and Hesperidin, Diosmin, Eriocitrin and ...

    African Journals Online (AJOL)

    Total Antioxidant Activity, and Hesperidin, Diosmin, Eriocitrin and Quercetin Contents of Various Lemon Juices. ... Conclusion: The developed method can also be applied to the determination of related extracts of orange cultivars as well as various extracts of medicinal plants. Keywords: Antioxidant, Flavonoid, Hesperidin, ...

  15. Antioxidant Activities and Food Value of Five Underutilized Green ...

    African Journals Online (AJOL)

    ... iron, calcium and vitamin C. The antioxidant activities and phenolic antioxidant contents of the vegetables were also high. The health claims associated with some of these food values and bioactive compounds are noteworthy, thereby underlining the potential role of these underutilized vegetables as functional foods.

  16. Antioxidant and sgc-7901 cell inhibition activities of Rhizoma ...

    African Journals Online (AJOL)

    The objective of this research was to study the pharmacology of Dioscorea bulbifera L. on antioxidant and anticancer activity. Alcohol extracts of Dioscorea bulbifera L. were made out by different concentration alcohol; they were tested by Hydroxyl radical scavenging test, reducing capacity test and total antioxidant capacity ...

  17. Effect of maturity and harvest season on antioxidant activity ...

    African Journals Online (AJOL)

    Antioxidant activity diphenylpicrylhydrazyl (DPPH), the ferric-reducing antioxidant power assay (FRAP), nitric oxide (NO)], total polyphenols, phenolic compounds and ascorbic acid of Morinda citrifolia L. fruits were investigated as a function of maturity and three seasons patterns in Mexico. Maturity was evaluated in early, ...

  18. Antioxidant and antimicrobial activities of Heracleum nepalense D ...

    African Journals Online (AJOL)

    Antioxidant and antimicrobial activities of Heracleum nepalense D Don root. S Dash, L K Nath, S Bhise. Abstract. Purpose: The aim of the present study was to investigate antioxidant and antimicrobial effects of the methanol extract of Heracleum nepalense D.Don roots. Method: The antimicrobial effect was determined by ...

  19. Hepatoprotective and antioxidant activities of fruit pulp of limonia ...

    African Journals Online (AJOL)

    Hepatoprotective and antioxidant activities of fruit pulp of limonia acidissima linn. ... The fruit extract significantly restored the antioxidant enzyme level in the liver cells. Conclusion: MELA exhibited significant dose ... This study rationalized the ethno-medicinal use of the plant for curing hepatic injuries. Keywords: Limonia ...

  20. Modulation of Antioxidant Enzyme Expression and Activity by ...

    African Journals Online (AJOL)

    Renal toxicity produced by paraquat involves the generation of reactive oxygen species (ROS) which can overwhelm antioxidant defences, leading to oxidant injury. However, there are conflicting reports regarding the activity and/or expression of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) ...

  1. Chemical composition and in vitro antioxidant activities of ...

    African Journals Online (AJOL)

    ... and the results were compared to the reference BHT (butyl hydroxy toluene). In the three earlier mentioned assays, the essential oil demonstrated a potential antioxidant which may be considered as potent agent in food preservation and drug discovery. Key words: Thymelaea hirsuta, essential oil, antioxidant activities.

  2. The antioxidant activity of selected Romanian honeys

    Directory of Open Access Journals (Sweden)

    Irina DOBRE

    2010-12-01

    Full Text Available Four types of Apis mellifera honey collected in the eastern region of Romania were screened for their total phenolic content by the Folin-Ciocalteau method, for anti radical power as assessed by DPPHradical scavenging assay, for the FRAP assay-ferric reducing antioxidant power. The antioxidant effect over a substrate sensitive to lipid per oxidation and also the presence of some pigments with antioxidant action in honey-ABS 450 were evaluated. All assays revealed the following order of the obtained values: Lime honey>poly-flower honey>Sea buckthorn honey>Acacia honey.

  3. The study of antioxidant activity of white tea

    Directory of Open Access Journals (Sweden)

    O. V. Chernousova

    2018-01-01

    Full Text Available Antioxidants protect the organism from the harmful effects of free radicals. High content of antioxidants is characteristic for red wine, olive oil, tea. Today in Russia is well studied the antioxidant activity of black and green tea, but the choice of teas found in the sale, much wider. The aim of the work was to study antioxidant activity of white tea, as well as its comparison with the parameters of green tea and rooibos. Spectrophotometrically compared the antioxidant activity of aqueous extracts white, green tea, drink rooibos using the adrenaline autooxidation reaction in alkaline medium (pH 10.65. The correctness of the spectrophotometric determination was confirmed on the device “Tsvet Yauza-01-AA” (Russia. As a standard used quercetin. In the initial period of time (up to 5 min tea extracts “Java”, «Elixir immortality» and rooibos are equally affect the values of optical density. After 10 min the optical density takes different values for different extracts, however this time is recommended to determine antioxidant activity. The highest antioxidant activity of the extract has white tea «Greenfield». Rooibos extract antioxidant activity could be compared with “Elixir of immortality” white tea аctivity. The lowest value of antioxidant activity of green tea “Princess JAVA”. The effect of water temperature on the antioxidant activity of extracts was investigated. The highest value was found in tea «Greenfield» at a brewing temperature of 70 °C, which decreases with increasing temperature to 100 °C. The water temperature has a minimal effect on the antioxidant activity of the rooibos extract, but a small maximum at 80 °C is observed. The minimum value of antioxidant activity is the extract of green tea «Princess JAVA», but it increases with the temperature rise up to 100 °C. The study was conducted according to the content of antioxidants from the brewing time. For this, the device “Tsvet Yauza-01-AA” was

  4. Antioxidant activity of the various extracts of cyclamen graecum link tubers and leaves from turkey

    International Nuclear Information System (INIS)

    Metin, H.; Aydin, C.; Mammadov, R.

    2013-01-01

    In this study, antioxidant activities of various solvent extracts (methanol, ethanol, acetone and petroleum benzine) obtained from tubers and leaves of Cyclamen graecum Link (Primulaceae) were determined. Antioxidant properties of various extracts from C. graecum were evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and beta-carotene-linoleic acid assays. In addition, total phenolic contents in all the extracts of C. graecum tubers and leaves were determined as pyrocatechol equivalents. Leaves extracts of C. graecum exhibited higher antioxidant activity than tuber extracts with all the types of solvent used. All extracts of C. graecum tubers and leaves had effective free radical scavenging activity. The scavenging activity of C. graecum leaf-ethanol extract (97.3 +- 0.55%) was found more effective than BHT (92.30 +- 0.35%). A positive correlation was observed between antioxidant activity and amount of phenolic contents of the extracts. In this study, all types of extracts obtained from C. graecum have showed strong antioxidant activity. Therefore, this species can be used as a natural antioxidant in food processing and pharmaceutical industries. (author)

  5. Effect of freeze-drying on the antioxidant compounds and antioxidant activity of selected tropical fruits.

    Science.gov (United States)

    Shofian, Norshahida Mohamad; Hamid, Azizah Abdul; Osman, Azizah; Saari, Nazamid; Anwar, Farooq; Dek, Mohd Sabri Pak; Hairuddin, Muhammad Redzuan

    2011-01-01

    The effects of freeze-drying on antioxidant compounds and antioxidant activity of five tropical fruits, namely starfruit (Averrhoa carambola L.), mango (Mangifera indica L.), papaya (Carica papaya L.), muskmelon (Cucumis melo L.), and watermelon Citruluss lanatus (Thunb.) were investigated. Significant (p 0.05) change, however, observed in the ascorbic acid content of the fresh and freeze-dried fruits. Similarly, freeze-drying did not exert any considerable effect on β-carotene concentration of fruits, except for mango and watermelon, where significantly (p correlation was established between the result of TPC and antioxidant assays, indicating that phenolics might have been the dominant compounds contributing towards the antioxidant activity of the fruits tested.

  6. Screening of antioxidant activity in microalgae

    Directory of Open Access Journals (Sweden)

    Mariana F.G. Assunção

    2014-06-01

    Both sets of results indicate an interesting antioxidant potential in microalgae belonging to the groups Eustigmatophyceae and Chlorophyceae. Tested species of these groups showed ABTS•+ values comparable to grape and raspberry ethanolic extracts, confirmed also by the DPPH• method.

  7. Anti-oxidation activity of different types of natural Cordyceps sinensis and cultured Cordyceps mycelia.

    Science.gov (United States)

    Li, S P; Li, P; Dong, T T; Tsim, K W

    2001-05-01

    Cordyceps, one of the well-known traditional Chinese medicines, consists of the dried fungus Cordyceps sinensis growing on the larva of the caterpillar. It is commonly used for the replenishment of body health. One of the known pharmacological effects is its anti-oxidation activity. However, there is a great variation of the quality in different sources of Cordyceps. Here, the water extracts of various sources of natural C. sinensis and cultured Cordyceps mycelia were analyzed for their anti-oxidation activity by using three different assay methods such as the xanthine oxidase assay, the induction of hemolysis assay and the lipid peroxidation assay. The results showed that Cordyceps, in general, possesses a strong anti-oxidation activity in all assays tested. However, both natural and cultured Cordyceps showed the lowest inhibition in the lipid peroxidation when compared with the other two assay methods. The cultured Cordyceps mycelia had equally strong anti-oxidation activity as compared to the natural Cordyceps. Besides, the anti-oxidation activities were increased to 10-30 folds in the partially purified polysaccharide fractions from the cultured Cordyceps mycelia, which suggested that the activity could be derived partly from Cordyceps polysaccharides.

  8. Antioxidant activity of fermented broccoli and spinach by Kombucha culture

    Science.gov (United States)

    Artanti, Nina; Susilowati, Agustine; Aspiyanto, Lotulung, Puspa Dewi Narrij; Maryati, Yati

    2017-11-01

    Broccoli (Brassica oleracea L.) and spinach (Amaranthus spp.) are vegetables that known to have many benefit for health. Previous studies on the fermentation of those vegetables using kombucha cultured showed increase in bioactive components such as total polyphenol content. The current studies was performed to evaluate the antioxidant activity of fermented spinach and broccoli before (feed) and after treatment with filtration (retentate and permeate). Filtration was conducted using Stirred Ultrafiltration Cell (SUFC) with UF membrane 100,000 MWCO mode at fixed condition (stirred rotation 300 rpm, room temperature, pressure 40 psia). Antioxidant evaluation was conducted using 2,2-diphenyl-1-picril hydrazyl (DPPH) free radical scavenging activity assay. The results showed that all samples from fermented broccoli showed antioxidant activity (feed 15.82% inhibition and retentate 15.29% inhibition), with the best antioxidant activity was obtained from permeate (75.98% inhibition). Whereas from fermented spinach only permeate showed antioxidant activity (21.84% inhibition) and it significantly lower than broccoli permeate. The mass spectrum of LCMS analysis on broccoli samples showed the present of several mass spectrum with (M+H) range from 148.1 to 442.5 in feed, retentate and permeate. In those samples (M+H) 360.4 always has the highest relative intensity. These results suggest that fermented broccoli has potential for development as functional drink for the source of antioxidant and the permeate obtained from filtration treatment significantly increased the antioxidant activity.

  9. Comparison of antioxidant activity in commercial Ginkgo biloba preparations.

    Science.gov (United States)

    Mantle, David; Wilkins, Richard M; Gok, Muhamed Asim

    2003-10-01

    To compare the relative levels of antioxidant activity in vitro in Ginkgo biloba samples (in tablet or capsule form) from different commercial suppliers, to determine whether some brands may be more efficacious in their potential to increase endogenous antioxidant activity, and thereby counter oxidative stress related disorders. Antioxidant activity of the above sample extracts was determined in vitro against the ABTS.(+) (2-2'-amino-bis-3-ethylbenzthiazoline-6-sulfonic acid) radical, relative to Trolox (water-soluble vitamin E analogue) antioxidant standards, using an established assay procedure. The relative antioxidant activity of G. biloba sample extracts was expressed in terms of millimoles per liter of Trolox equivalent (TE) for the initial extract, micromol TE per whole tablet, nmol TE per mg tablet, and nmol TE per mg ginkgo content. Data (as mean +/- standard deviation (SD) from 4 separate estimations) obtained in this study showed a considerable variation (approximately 50-fold) in the level of antioxidant activity in preparations from different suppliers, particularly when compared on an equivalent (i.e., nmol TE/mg ginkgo) basis. Of the 18 products investigated, the highest level of antioxidant activity (whether expressed as micromol TE per whole tablet or nmol TE/mg ginkgo) was obtained for Pharma Nord Bio-Biloba (Pharma Nord, Morpeth, UK) tablets (p antioxidant activity of the various products investigated can be accounted for in terms of the physical nature of the G. biloba (i.e., dried leaf powder or standardized concentrated extract) used in tablet formulation. However, even when comparing products based on concentrated extract, the data demonstrated that there is still a considerable variation in antioxidant activity. Presumably this may result from differences in the manufacturing process between suppliers, which in turn may limit the efficacy of these preparations in the prevention or treatment of disease.

  10. Application of radiobiological techniques in studying antioxidant mechanisms: evaluation of their radioprotective, antioxidative and antiviral activities

    International Nuclear Information System (INIS)

    Hmamouchi, M.

    2000-01-01

    In the medical field, the oxidation phenomenon is the source of several pathologies (diabetes, cystic fibrosis, cancers,...). The natural oxidants are used as food preserving and skin ageing moderators. Several plant extracts with antioxidant activity were studied, this important antioxidant activity is probably due to their richness of compounds: polyphenols, phenolic acids, tocopherols, carotenoids, flavonoids,... Many techniques for evaluation and reactional mechanism study of the antioxidative activity are used. After selection, extraction, fractionation, activity screening, chemical analyses of molecules contained in the best active extracts, biological properties research of isolated redox pharmacophore, we have : - determined the structure of active products by spectroscopy and chromatography; - studied the antioxidative properties by EPR and spin trapping of the obtained extracts and molecules. The results of this first part of our work consists in evaluating the antioxidative degree of a great number of natural active principles, extracted from moroccan plants and pur obtained products. The second part consists in studying the action mechanisms using the LDL labelling (F. M.)

  11. Antioxidant Activity, Total Phenolic and Flavonoid Contents of Three Onobrychis Species from Iran

    Directory of Open Access Journals (Sweden)

    Roya Karamian , Mostafa Asadbegy

    2016-06-01

    Full Text Available Background: Plant phenolic compounds are a main group of plant natural products and flavonoids are the largest and best studied natural phenols. These substances possess a series of biological properties and act on biological systems as antioxidants. In present research, the aim is to determine in vitro total phenolics content and antioxidant activity of the methanolic extracts of three Onobrychis species belonging to the family Fabaceae, namely O. sosnovskyi Grossh., O. viciifolia Scop. and O. melanotricha Boiss. Furthermore, an attempt was made to identify any correlations between total phenolic content of the extracts with their antioxidant activities Methods: Total phenol and flavonoid contents of the extracts were determined by Folin-Ciocalteu and aluminum chloride methods, respectively. Antioxidant activity was evaluated by three different test systems, namely 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging (DPPH, metal-chelation activity and ß-carotene/linoleic acid model. Results: Results indicated that O. viciifolia extract contains the highest total phenolic content (10.38 ± 0.33mg GAE/g of dry extract. However, the species are not remarkable different (P 0.55 to 0.98. Conclusion: Our results showed that the examined Onobrychis extracts represent strong antioxidant activity; hence, they can be suggested as antioxidant agents for special use in future.

  12. Effect of gamma irradiation on antioxidant activity of Amoora rohitaka

    International Nuclear Information System (INIS)

    Rajurkar, N.S.; Gaikwad, K.N.

    2012-01-01

    The effect of a medium dose of gamma radiation on antioxidant activity of Amoora rohitaka was studied. Radiation doses were 0, 1, 3 and 5 kGy. Antioxidant activity was screened by using different assay. With increasing dose the formation of Maillard reaction products (MRPs) contributes to the increase in the antioxidant activity. MRPs are formed as a result of Maillard reaction. In ABTS [2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid di-ammonium salt] assay, ethanol extract showed increase in scavenging activity. It also showed a marked increase in polyphenolic contents. The present study reveals that gamma irradiation can be an efficient process to increase antioxidant activity of Amoora rohitaka. (author)

  13. Synthesis and Antioxidant Activity of Alkyl Nitroderivatives of Hydroxytyrosol

    Directory of Open Access Journals (Sweden)

    Elena Gallardo

    2016-05-01

    Full Text Available A series of alkyl nitrohydroxytyrosyl ether derivatives has been synthesized from free hydroxytyrosol (HT, the natural olive oil phenol, in order to increase the assortment of compounds with potential neuroprotective activity in Parkinson’s disease. In this work, the antioxidant activity of these novel compounds has been evaluated using Ferric Reducing Antioxidant Power (FRAP, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS, and Oxygen Radical Scavenging Capacity (ORAC assays compared to that of nitrohydroxytyrosol (NO2HT and free HT. New compounds showed variable antioxidant activity depending on the alkyl side chain length; compounds with short chains (2–4 carbon atoms maintained or even improved the antioxidant activity compared to NO2HT and/or HT, whereas those with longer side chains (6–8 carbon atoms showed lower activity than NO2HT but higher than HT.

  14. Antioxidant gap and lipid peroxidation in patients with rheumatoid arthritis: Relationship to disease manifestations and activity

    Directory of Open Access Journals (Sweden)

    Anuradha B. Patil

    2012-10-01

    Full Text Available Objective: To explore relationships between total antioxidant gap and lipid peroxidation with respect to the disease severity of Rheumatoid arthritis (RA, which may have further implications in understanding rheumatoid pathology and therapeutic management of the disease. Method: The present of study was designed to investigate the relationship between antioxidant gap and certain antioxidant parameters with disease activity in rheumatoid arthritis patients. A significantly increased lipid peroxidation, measured as malondialdehyde (MDA, was demonstrated in the plasma of rheumatoid arthritis patients. Results: MDA was observed in RA patients (0.98暲0.34 毺 mol/L than those found in controls (0.98暲0.34 毺 mol/L. The antioxidant gap was significantly decreased. total antioxidant gap (0.34暲0.14 mmol/L and total antioxidant capacity (1.34 暲0.16 mmol/L were significantly lower in RA patients as compared to healthy controls (0.76暲0.33 and 1.78暲0.35 mmol/Lrespectivel. Discussion: The excessive production of ROS disturbs redox status including antioxidant gap and can exacerbating inflammation and affecting tissue damage in RA, as exemplified by their strong association with disease activity

  15. Effect of ionizing radiation on antioxidants and antibacterial activities of Inula Viscasa

    International Nuclear Information System (INIS)

    Rhimi, W.; Issam, B; Saidi, M; Abdennacer, B; Maroua, J

    2015-01-01

    In the present study, the irradiation processing of Tunisian Inula Viscosa samples was carried out at dose of 5 kGy. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging activity, the azinobis ethylbenzothiazoline 6-sulphonic acid (ABTS) Ferric reducing antioxidant power (FRAP) assay and the antibacterial activities of both control and irradiated samples extracted in methanol and ethanol were evaluated. The results showed that the irr Tunisian Inula Viscosa extracts had strong antioxidant ability. The scavenger DPPH, ABTS and FRAP values of all extracts decreased significantly after irradiation. In addition, all extracts were effective against all the gram positive and gram negative pathogens. Gamma irradiation preserved the antibacterial activities of extracts and enhanced significantly (p≺0.05) the activity of extracts against E.coli. These data indicated the potential use of gamma-irradiation as a safe technique for preservation of Inula Viscosa as a medicinal plant with effective antioxidant and antibacterial activities

  16. Chemical Composition, Antioxidant, and Antimicrobial Activities of Lichen Umbilicaria cylindrica (L. Delise (Umbilicariaceae

    Directory of Open Access Journals (Sweden)

    Nedeljko T. Manojlovic

    2012-01-01

    Full Text Available The phytochemical analysis of methanol and chloroform extracts of Umbilicaria cylindrica was determined by HPLC-UV method. The predominant phenolic compound in both extracts was depsidone, salazinic acid (1. Besides salazinic acid, the tested extracts of U. cylindrica contain norstictic acid (2, methyl-β-orcinol carboxylate (3, ethyl haematommate (4, atranorin (5, and usnic acid (6, in different amounts and relations. The lichen extracts showed comparable and strong antioxidant activity, exhibited higher DPPH and hydroxyl radical scavengings, chelating activity, and inhibitory activity towards lipid peroxidation. The lichen extracts demonstrated important antimicrobial activity against eight strains with MIC values from 15.62 to 62.50 μg/mL. This is the first report of the detail chemical composition and antioxidant activity of the lichen Umbilicaria cylindrica, and the results suggest that this lichen can be used as a new source of the natural antioxidants and the substances with antimicrobial features.

  17. Isolation, identification and activity of natural antioxidants from horehound (Marrubium vulgare L.) cultivated in Lithuania

    NARCIS (Netherlands)

    Pukalskas, A.; Venskutonis, P.R.; Salido, S.; Waard, de P.; Beek, van T.A.

    2012-01-01

    In an earlier screening of Lithuanian plants, horehound (Marrubium vulgare) showed good antioxidant activity and as this species is used in herbal teas and cough pastilles it was selected for further investigation. Some fractions of the aerial parts were strong scavengers of the model free radicals

  18. Antioxidant activity of leaf extracts from different Hibiscus sabdariffa accessions and simultaneous determination five major antioxidant compounds by LC-Q-TOF-MS.

    Science.gov (United States)

    Wang, Jin; Cao, Xianshuang; Jiang, Hao; Qi, Yadong; Chin, Kit L; Yue, Yongde

    2014-12-17

    Hibiscus sabdariffa has gained attention for its antioxidant activity. There are many accessions of H. sabdariffa in the world. However, information on the quantification of antioxidant compounds in different accessions is rather limited. In this paper, a liquid chromatography/quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS) method for simultaneous determination of five antioxidant compounds (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, and isoquercitrin) in H. sabdariffa leaves was developed. The method was validated for linearity, sensitivity, precision, repeatability and accuracy. The validated method has been successfully applied for determination of the five analytes in eight accessions of H. sabdariffa. The eight accessions of H. sabdariffa were evaluated for their antioxidant activities by DPPH free radical scavenging assay. The investigated accessions of H. sabdariffa were rich in rutin and exhibited strong antioxidant activity. The two accessions showing the highest antioxidant activities were from Cuba (No. 2) and Taiwan (No. 5). The results indicated that H. sabdariffa leaves could be considered as a potential antioxidant source for the food industry. The developed LC-Q-TOF-MS method is helpful for quality control of H. sabdariffa.

  19. Enhanced antioxidant activity of polyolefin films integrated with grape tannins.

    Science.gov (United States)

    Olejar, Kenneth J; Ray, Sudip; Kilmartin, Paul A

    2016-06-01

    A natural antioxidant derived from an agro-waste of the wine industry, grape tannin, was incorporated by melt blending into three different polyolefins (high-density polyethylene, linear low-density polyethylene and polypropylene) to introduce antioxidant functionality. Significant antioxidant activity was observed at 1% tannin inclusion in all polymer blends. The antioxidant activity was observed to increase steadily with a greater concentration of grape tannins, the highest increases being seen with polypropylene. The mechanical and thermal properties of the polymer films following antioxidant incorporation were minimally altered with up to 3% grape tannins. All of the polyolefin-grape tannin films successfully passed the leachability test following USP661 standard protocol. Superior antioxidant activity was established in polyolefin thin films by utilization of a bulk grape extract obtained from winery waste. Significant increases in antioxidant activity were seen with 1% extract inclusion. This not only demonstrates the potential for food packaging applications of the polyolefin blends, but also valorizes the agro-waste. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

  20. Antioxidant activity in selected Slovenian organic and conventional crops

    Directory of Open Access Journals (Sweden)

    Manca KNAP

    2015-12-01

    Full Text Available The demand for organically produced food is increasing. There is widespread belief that organic food is substantially healthier and safer than conventional food. According to literature organic food is free of phytopharmaceutical residues, contain less nitrates and more antioxidants. The aim of the present study was to verify if there are any differences in the antioxidant activity between selected Slovenian organic and conventional crops. Method of DPPH (2,2-diphenyl-1-picryhydrazyl was used to determine the antioxidant activity of 16 samples from organic and conventional farms. The same varieties of crops were analysed. DPPH method was employed to measure the antioxidant activity of polar antioxidants (AAp and antioxidant activity of fraction in ethyl acetate soluble antioxidants (EA AA. Descriptive statistics and variance analysis were used to describe differences between farming systems. Estimated differences between interactions for the same crop and different farming practice were mostly not statistically significant except for the AAp for basil and beetroot. Higher statistically significant values were estimated for conventional crops. For the EA AA in broccoli, cucumber, rocket and cherry statistically significant higher values were estimated for organic production.

  1. A Caco-2 cell-based quantitative antioxidant activity assay for antioxidants.

    Science.gov (United States)

    Wan, Hongxia; Liu, Dong; Yu, Xiangying; Sun, Haiyan; Li, Yan

    2015-05-15

    A Caco-2 cell-based antioxidant activity (CAA) assay for quantitative evaluation of antioxidants was developed by optimizing seeding density and culture time of Caco-2 cells, incubation time and concentration of fluorescent probe (2',7'-dichlorofluorescin diacetate, DCFH-DA), incubation way and incubation time of antioxidants (pure phytochemicals) and DCFH-DA with cells, and detection time of fluorescence. Results showed that the CAA assay was of good reproducibility and could be used to evaluate the antioxidant activity of antioxidants at the following conditions: seeding density of 5 × 10(4)/well, cell culture time of 24h, co-incubation of 60 μM DCFH-DA and pure phytochemicals with Caco-2 cells for 20 min and fluorescence recorded for 90 min. Additionally, a significant correlation was observed between CAA values and rat plasma ORAC values following the intake of antioxidants for selected pure phytochemicals (R(2) = 0.815, p < 0.01), demonstrating the good biological relevance of CAA assay. Copyright © 2014 Elsevier Ltd. All rights reserved.

  2. Antioxidative and Hepatoprotective Activities of Deinoxanthin-Rich ...

    African Journals Online (AJOL)

    Antioxidative and Hepatoprotective Activities of Deinoxanthin-Rich Extract from Deinococcus radiodurans R1 against Carbon Tetrachloride-Induced Liver Injury in Mice. J Cheng, Z Zhang, Z Zheng, G Lv, L Wang, B Tian, Y Hua ...

  3. Antioxidant and antibacterial activities of ethanolic extracts of ...

    African Journals Online (AJOL)

    Antioxidant and antibacterial activities of ethanolic extracts of Asparagus officinalis cv. Mary Washington: Comparison of in vivo and in vitro grown plant bioactivities. Arash Khorasani, Wirakarnain Sani, Koshy Philip, Rosna Mat Taha, Arash Rafat ...

  4. Antioxidant activity of black bean (Phaseolus vulgaris L. protein hydrolysates

    Directory of Open Access Journals (Sweden)

    Jarine Amaral do EVANGELHO

    2016-01-01

    Full Text Available Abstract The objective of this work was to study the effect of enzymatic hydrolysis of black bean protein concentrate using different enzymes. Bean proteins were extracted and hydrolyzed over a period of 120 min using the enzymes pepsin or alcalase. The protein hydrolysates’ molecular weight was assayed by electrophoresis and the antioxidant activity was evaluated by the capturing methods of free radicals ABTS●+ and DPPH. Electrophoretic results showed that the bands above 50 kDa disappeared, when the beans protein was subjected to hydrolysis with pepsin. The bean protein hydrolysate obtained by hydrolysis with alcalase enzyme, showed higher antioxidant activity for inhibition of the radical ABTS●+. However, the hydrolysates obtained by hydrolysis with pepsin had higher antioxidant activity for inhibition of the radical DPPH. The use of pepsin and alcalase enzymes, under the same reaction time, produced black bean protein hydrolysates with different molecular weight profiles and superior antioxidant activity than the native bean protein.

  5. Antioxidant activities of the selected plants from the family ...

    African Journals Online (AJOL)

    . Antioxidant activities of these extracts were evaluated through DPPH• radical scavenging, phosphomolybdate and ferric thiocyanate (FTC) methods. Methanolic extract of Cinnamomum zeylanicum and Cinnamomum tamala showed highest ...

  6. In vitro Cytotoxic and Antioxidant Activity of Leaf Extracts of ...

    African Journals Online (AJOL)

    In vitro Cytotoxic and Antioxidant Activity of Leaf Extracts of Mangrove Plant, Phoenix paludosa Roxb. Sameera R. Samarakoon, Chanthirika Shanmuganathan, Meran K. Ediriweera, Kamani H. Tennekoon, Poorna Piyathilaka, Ira Thabrew, E Dilip de Silva ...

  7. Purification, composition analysis and antioxidant activity of different ...

    African Journals Online (AJOL)

    Purification, composition analysis and antioxidant activity of different polysaccharides from the fruiting bodies of Pholiota adiposa. Yongxin Nie, Hongxia Jiang, Yanyou Su, Changxiang Zhu, Jiarui Li, Fujiang Wen ...

  8. Antioxidant, antimicrobial and neutrophil-modulating activities of herb extracts

    Czech Academy of Sciences Publication Activity Database

    Denev, P.; Kratchanova, M.; Číž, Milan; Lojek, Antonín; Vašíček, Ondřej; Blazheva, D.; Nedelcheva, P.; Vojtek, L.; Hyršl, P.

    2014-01-01

    Roč. 61, č. 2 (2014), s. 359-367 ISSN 0001-527X Institutional support: RVO:68081707 Keywords : herbs * polyphenols * antioxidant activity Subject RIV: BO - Biophysics Impact factor: 1.153, year: 2014

  9. Antibacterial and Antioxidant Activities of Liquidambar Orientalis Mill. Various Extracts Against Bacterial Pathogens Causing Mastitis

    Directory of Open Access Journals (Sweden)

    Gülten Ökmen

    2017-08-01

    Full Text Available Antibiotic resistance is being constantly developed worldwide. Coagulase Negative Staphylococci (CNS and Staphylococcus aureus are common causes of bovine subclinical mastitis. Bioactive compound of medicinal plants shows anti-microbial, anti-mutagenic and anti-oxidant effects. The anti-bacterial and anti-oxidant activities of Liquidambar orientalis (L. orientalis extracts on subclinical mastitis causing bacteria in cows have not been reported to date. The aim of the present study was to examine anti-bacterial and anti-oxidant effects of L. orientalis leaf extracts on S. aureus and CNS isolated from cows with subclinical mastitis symptoms. In this study, 3.2 mg/mL minimum inhibitory concentration (MIC of ethanol extracts of L. orientalis has shown to be a most potent anti-bacterial and anti-oxidant for all isolated bacterial species from mastitis cows. In this study, it was investigated anti-bacterial and anti-oxidant potentials of acetone, methanol and ethanol extracts of the L. orientalis. The acetone extract showed maximum inhibition zone against S. aureus numbered 17 (12 mm. In addition to anti-bacterial properties, anti-oxidant activity of L. orientalis extract was examined by ABTS [2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid] free radical assay. Trolox was used as a positive control anti-oxidant. Ethanol extract exhibited a strong anti-oxidant activity like Trolox anti-oxidant which was effective at 2.58 mM concentration. Bioactive compounds of sweet gum may be useful to screening mastitis causing bacteria for clinical applications.

  10. Antioxidant Activity of Water-soluble Polysaccharides from Brasenia schreberi

    OpenAIRE

    Xiao, Huiwen; Cai, Xueru; Fan, Yijun; Luo, Aoxue

    2016-01-01

    Objective: In order to investigate the antioxidant activities of polysaccharides (BPL-1 and BPL-2), one of the most important functional constituents in Brasenia schreberi was isolated from the external mucilage of B. schreberi (BPL-1) and the plant in vivo (BPL-2). This paper examines the relationship between the content of sulfuric radicals and uronic acid in BPL and the antioxidant activity of BPL. Materials and Methods: The free radicals, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic ...

  11. [Effect of antioxidants with different antiradical activity on cornea preservation].

    Science.gov (United States)

    Travkin, A G; Vinetskaia, M I; Slepukhina, L V

    1976-11-01

    Changes of chemoluminescence intensity, collagen mucopolysaccaride content in the rabbit eye cornea were studied on 32 cornea of shinshilla eye conservated by dehydration under hipotenia (2 divided by 4 degrees C) for 60 days with the application of antioxidants (tocoferol, chlorhydrate 2-ethyl, 6-methyl-3-oxipyridine) having different antiradical activity. It is concluded that the application of natural antioxidant alpha-tocoferol having high antiradical activity is expedient for the conservation of cornea.

  12. Antioxidative and Anticholinesterase Activity of Cyphomandra betacea Fruit

    OpenAIRE

    Ali Hassan, Siti Hawa; Abu Bakar, Mohd Fadzelly

    2013-01-01

    Cyphomandra betacea is one of the underutilized fruits which can be found in tropical and subtropical countries. This study was conducted to determine the antioxidant activity and phytochemical contents in different parts (i.e., flesh and peel) of the fruits. Antioxidants were analyzed using DPPH and ABTS free radical scavenging assays as well as FRAP assay. Anticholinesterase activity was determined using enzymatic assay using acetyl cholinesterase enzyme. For 80% methanol extract, the peel ...

  13. Phytochemical study, antioxidant and antibacterial activities of Stemodia maritima

    Directory of Open Access Journals (Sweden)

    Francisca R. L. da Silva

    2014-01-01

    Full Text Available Stemodinol, a new natural compound, together with known compounds including jaceidin, stemodin, stemodinoside B, isocrenatoside, verbascoside, crenatoside, and isoverbascoside, were isolated from Stemodia maritima Linn. The antioxidant (DPPH method and antimicrobial activities of stemodin, stemodinoside B, and crenatoside were investigated. Among the components tested, only crenatoside isolated from the roots showed a high antioxidant power. Stemodin and stemodinoside B exhibited antibacterial activities.

  14. Edible flowers - antioxidant activity and impact on cell viability

    OpenAIRE

    Kuceková, Zdenka; Mlček, Jiří; Humpolíček, Petr; Rop, Otakar

    2013-01-01

    The phenolic compound composition, antioxidant activity and impact on cell viability of edible flower extracts of Allium schoenoprasum; Bellis perennis; Cichorium intybus; Rumex acetosa; Salvia pratensis; Sambucus nigra; Taraxacum officinale; Tragopogon pratensis; Trifolium repens and Viola arvensis was examined for the first time. Total phenolic content of the flowers of these plants fell between 11.72 and 42.74 mg of tannin equivalents/kg of dry matter. Antioxidant activity ranged from 35.5...

  15. Antioxidant activity of commonly consumed vegetables in yemen.

    Science.gov (United States)

    Al-Mamary, Mohammed Ali

    2002-09-01

    Of late, more attention has been paid to the role of natural antioxidants, especially phenolic compounds, which may act both by reducing the content of toxic compounds in foods and by supplying the human body with exogenous antioxidants. The total phenolic contents and the total antioxidant activities (% AA) of 17 commonly consumed vegetables in Yemen were determined. Total phenolics was measured colorimetrically using the Folin-Ciocalteu reagent with D-catechin as the standard. The antioxidant activity (%AA) of vegetables using their juices at different levels (50μl, 100μl, 200μl) was assayed in vitro by the inhibition of liver homogenate oxidation mediated by FeSO4 / ascorbate system. The % AA was measured by the TBAR method. The results showed that for all vegetable juices, increased antioxidant activity or decreased prooxidant activity increased with volume of juice. With the addition of 50μl from each sample, most vegetable juices showed antioxidant activity and it appears that coriander had exceptionally high antioxidant activity (60%). However, garlic, yellow onion, parsley, red onion and Cissus rotundifolia showed considerably high antioxidant activities (54-41 %). Other vegetables with low antioxidant activities (27 to 3 %) showed an order of increase in %AA as follows: tomato > spinach > horseradish-leaves > carrot > cabbage > chilli > leek > horseradish-roots. At the same, time some vegetable juices such as that of green pepper, cucumber and lettuce showed initial prooxidant activities (-11 to -40 %). The complete inhibition of oxidation was noticed when the highest level of juices (200μl) was used in the cases of coriander, onions, garlic and parsley. The correlation between total phenolics and the antioxidant activities of vegetables with different levels of juices (50μl, 100μl and 200μl) showed positive but not significant correlations (R = 0.41 - 0.55). Some vegetables such as carrots and tomatoes show the highest relative antioxidant

  16. Phenolic profile and antioxidant activities of olive mill wastewater.

    Science.gov (United States)

    El-Abbassi, Abdelilah; Kiai, Hajar; Hafidi, Abdellatif

    2012-05-01

    Olive trees play an important role in the Moroccan agro-economy, providing both employment and export revenue. However, the olive oil industry generates large amounts of wastes and wastewaters. The disposal of these polluting by-products is a significant environmental problem that needs an adequate solution. On one hand, the phytotoxic and antimicrobial effects of olive mill wastewaters are mainly due to their phenolic content. The hydrophilic character of the polyphenols results in the major proportion of natural phenols being separated into the water phase during the olive processing. On other hand, the health benefits arising from a diet containing olive oil have been attributed to its richness in phenolic compounds that act as natural antioxidants and are thought to contribute to the prevention of heart diseases and cancers. Olive mill wastewater (OMW) samples have been analysed in terms of their phenolic constituents and antioxidant activities. The total phenolic content, flavonoids, flavanols, and proanthocyanidins were determined. The antioxidant and radical scavenging activity of phenolic extracts and microfiltred samples was evaluated using different tests (iron(II) chelating activity, total antioxidant capacity, DPPH assays and lipid peroxidation test). The obtained results reveal the considerable antioxidant capacity of the OMW, that can be considered as an inexpensive potential source of high added value powerful natural antioxidants comparable to some synthetic antioxidants commonly used in the food industry. Copyright © 2011 Elsevier Ltd. All rights reserved.

  17. Antioxidant and antimicrobial activities of selected medicinal plants from Algeria

    Directory of Open Access Journals (Sweden)

    Krimat Soumia

    2014-06-01

    Full Text Available Objective: To evaluate the antioxidant and antimicrobial activity of methanolic extract extracts of selected Algerian medicinal plants. Methods: Antioxidant activity of extracts was evaluated in terms of radical scavenging potential (2,2-diphenyl-1-picrylhydrazyl and β-carotene bleaching assay. Total phenolic contents and flavonoid contents were also measured. Antimicrobial activity of these plants was examined against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. Results: The values of IC50 ranged from 4.30 μg/mL to 486.6 μg/mL for the DPPH method, while total antioxidant activity using β-carotene/linoleic acid bleaching assay ranged from 17.03% to 86.13%. It was found that Pistacia lentiscus showed the highest antioxidant capacities using DPPH assay (IC50=4.30 μg/mL, while Populus trimula, Origanum glandulosum, Centaurea calcitrapa, Sysimbrium officinalis and Rhamnus alaternus showed the highest percent of total antioxidant activity in β-carotene/linoleic acid bleaching assay. Total phenolic and flavonoid contents ranged from 3.96 to 259.65 mg GAE/g extract and from 1.13 to 26.84 mg QE/g extract, respectively. The most interesting antimicrobial activity was obtained from Sysimbrium officinalis, Rhamnus alaternus, Origanum glandulosum, Cupressus sempervirens, Pinus halipensis and Centaurea calcitrapa. Conclusions: The results indicated that the plants tested may be potential sources for isolation of natural antioxidant and antimicrobial compounds.

  18. Cellulase-assisted extraction and antioxidant activity of the polysaccharides from garlic.

    Science.gov (United States)

    Pan, Saikun; Wu, Shengjun

    2014-10-13

    In the present study, the polysaccharides were prepared from garlic by using a cellulase-assisted extraction method and the antioxidant activity of garlic polysaccharides (GPs) was evaluated. To improve the yield of GPs, the influences of the several factors such as extraction time, temperature, pH, and cellulase amount on the extraction efficiency were studied. The optimal conditions for extraction of GPs were determined as follows: time, 80 min; temperature, 45 °C; pH, 5; cellulase amount, 8000 U/g. Under the optimised extraction conditions, the yield of GPS reached up to 35.34%. The GPs product exhibited strong antioxidant activity including hydroxyl radical scavenging activity, 2,2-diphenyl-β-picrylhydrazyl radical scavenging activity, and reducing power. The results suggest that the GPs could be used as potential antioxidants. Copyright © 2014 Elsevier Ltd. All rights reserved.

  19. Chemical compositions and antioxidant activities of water extracts of Chinese propolis.

    Science.gov (United States)

    Guo, Xiali; Chen, Bin; Luo, Liping; Zhang, Xi; Dai, Ximo; Gong, Shangji

    2011-12-14

    The present study investigated the chemical composition and antioxidant activity of the water extract of propolis (WEP) collected from 26 locations in China. Spectrophotometry was used to determine the physicochemical properties and the chemical constituents of WEP. Phenolic compounds in WEP were identified by RP-HPLC-DAD with reference standards. The antioxidant activities [characterized by reducing power and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging ability] of WEP were also measured. Results show that epicatechin, p-coumaric acid, morin, 3,4-dimethoxycinnamic acid, naringenin, ferulic acid, cinnamic acid, pinocembrin, and chrysin are the major functional phenolic compounds in Chinese WEPs. Furthermore, most WEPs show strong antioxidant activities, which are significantly correlated with E(1cm)(1%), an index for the estimation of the quality of WEP. WEPs also contain many more active constituents than ethanol extracts of propolis.

  20. Comparative assessment of antioxidant activity of chicory products

    Directory of Open Access Journals (Sweden)

    Z. A. Badretdinova

    2016-01-01

    Full Text Available There are various methods for determining the antioxidant activity. They are differing by the source of oxidation, oxidized compounds and methods for the measurement of oxidized compounds. Methods for determination of antioxidant activity provide a wide range of results, so the evaluation of the antioxidant activity should be carried out in several ways, and the results should be interpreted with caution. In assessing antioxidant capacity is necessary to consider not only the nature and content of the reducing agent in the test facility, but also the possibility of their mutual influence. The objects of research in this research work were Instant chicory (Leroux, Fried chicory (Leroux, Instant chicory (LLC Favorit, Instant chicory (LLC SlavKofe, Instant chicory with hawthorn (Iceberg Ltd and K, Instant chicory (LLC Flagistom, Instant chicory (Ltd. Around the World, Instant chicory (LLC Beta +, Chicory flour (Leroux, Chicory flour. Antioxidant activity of the products of chicory determined by spectrophotometric (using adrenaline and 2,2 – diphenyl- 1-picrylhydrazyl, and amperometric methods. These results indicate that chicory products possess antioxidant activity but not give fully correlated with each other results. The highest antioxidant activity have fried products from chicory – Instant chicory (LLC SlavKofe and Instant chicory with hawthorn (Iceberg Ltd and K. No fried products from chicory (Chicory flour (Leroux have lower antioxidant activity then fried products from chicory. Because during frying new compounds are formed – chikoreol, which comprises acetic and valeric acid, acrolein and furfural, furfuryl alcohol, diacetyl. Best convergence results provide spectrophotometric methods using epinephrine and 2,2 – diphenyl-1-picrylhydrazyl.

  1. Antimicrobial and antioxidant activity of lemon balm Kombucha

    Directory of Open Access Journals (Sweden)

    Velićanski Aleksandra S.

    2007-01-01

    Full Text Available Kombucha is a beverage traditionally produced by metabolic activity of yeasts and acetic acid bacteria. The antimicrobial activity of lemon balm kombucha as well as of particular control samples was determined by agar-well diffusion method. Antioxidant activity on stable 1,1-diphenyl-2-picrylhydrazyl radicals of lemon balm kombucha and lemon balm tea was determined by electron spin resonance spectroscopy. Acetic acid, Kombucha samples and heat-denaturated kombucha showed significant antimicrobial activity against bacteria. However, there was no activity against yeasts and moulds. Kombucha showed higher antioxidant activity than tea sample for all applied sample volumes.

  2. Correlations between polyphenolic composition and antioxidant activity of Venetian propolis.

    Science.gov (United States)

    Gregoris, Elena; Stevanato, Roberto

    2010-01-01

    Four propolis samples have been picked up in the Venetian region, from different orography and habitative density areas with the purpose to: (i) evaluate propolis' antioxidant activity, measured by inhibition of lipid peroxidation; (ii) determine the polyphenolic components--flavonoids and caffeic acid derivatives--which give antioxidant activity to propolis; (iii) verify the potential correlations between antioxidant activity, polyphenolic content, that has been determined by Folin-Ciocalteu, enzymatic, DPPH quenching, TEAC-like assays, and spectroscopic characteristics of propolis and (iv) correlate chemical structure and antioxidant efficacy of each of the major components. The possible localization of the lipophylic components of propolis into the phospholipidic bilayer by thermal analysis (DSC) and spin label EPR techniques has also been investigated. Copyright 2009 Elsevier Ltd. All rights reserved.

  3. Antioxidant Activity of Marine Algal Polyphenolic Compounds: A Mechanistic Approach.

    Science.gov (United States)

    Fernando, I P Shanura; Kim, Misook; Son, Kwang-Tae; Jeong, Yoonhwa; Jeon, You-Jin

    2016-07-01

    Polyphenolic compounds isolated from marine algae exhibit a broad spectrum of beneficial biological properties, including antioxidant, anticancer, antimicrobial, anti-inflammatory, and antidiabetic activities, along with several other bioactivities centered on their antioxidant properties. Consequently, polyphenolic compounds are increasingly being investigated for their potential use in food, cosmetic, and pharmaceutical applications. The antioxidant activities of these compounds have been explored widely through experimental studies. Nonetheless, a theoretical understanding of the structural and electronic properties could broaden research perspectives, leading to the identification and synthesis of efficient structural analogs with prophylactic uses. This review briefly summarizes the current state of knowledge regarding antioxidant polyphenolic compounds in marine algae with an attempt to describe the structure-activity relationship.

  4. Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms.

    Science.gov (United States)

    Zhang, Lan; Hu, Yu; Duan, Xiaoyu; Tang, Tingting; Shen, Yingbin; Hu, Bin; Liu, Aiping; Chen, Hong; Li, Cheng; Liu, Yuntao

    2018-02-16

    Water-soluble polysaccharides were extracted from the caps and stipes of thirteen boletus mushrooms representing five different species collected in Southwest China. Investigations of their structures and antioxidant activities allowed an evaluation of structure-function relationships. The polysaccharides were composed mainly of the monosaccharides arabinose, xylose, mannose, glucose and galactose. Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes. FT-IR spectral analysis of the polysaccharides revealed that most of polysaccharides from boletus mushrooms (except Boletus edulis) contained a pyranose ring. The antioxidant activities of the polysaccharides in stipes showed a significant correlation with their monosaccharide composition, and were also related to their molecular weight and anomeric configuration. Suillellus luridus collected in Pingwu, Mianyang, Sichuan, China had remarkably superior antioxidant activity and might be developed as a natural antioxidant. Copyright © 2018 Elsevier B.V. All rights reserved.

  5. Antioxidant activity of organic extracts from aqueous infusions of sage.

    Science.gov (United States)

    Matsingou, Triantafillia Christina; Petrakis, Nicolaos; Kapsokefalou, Maria; Salifoglou, Athanasios

    2003-11-05

    The antioxidant activity of aqueous infusions of sage emerges from specific components present in that herb. In an attempt to investigate the chemical nature and properties of these components, four organic solvent extracts from aqueous infusions of sage were examined. HPLC analyses of these extracts led to the separation of a number of components, of which four were identified and quantified through the use of standard compounds of known chromatographic HPLC profiles. These compounds are the diterpenes carnosic acid, carnosol, and rosmanol and the hydroxycinnamic acid caffeic acid. The antioxidant activity and polyphenol content were determined in the four organic solvent extracts and the left-over aqueous fraction. Both polyphenolic and nonpolyphenolic substances present in the extracts arise as significant contributors to the observed antioxidant activity of the derived extracts and thus sage itself. In this sense, they reflect the antioxidant potential of the aqueous infusions of sage toward reactive oxygen species generated through variable mechanisms of iron-promoted oxidative processes.

  6. Antioxidant and Anti-Adipogenic Activities of Trapa japonica Shell Extract Cultivated in Korea.

    Science.gov (United States)

    Lee, DooJin; Lee, Ok-Hwan; Choi, Geunpyo; Kim, Jong Dai

    2017-12-01

    Trapa japonica shell contains phenolic compounds such as tannins. Studies regarding the antioxidant and anti-adipogenic effects of Trapa japonica shell cultivated in Korea are still unclear. Antioxidant and anti-adipogenic activities were measured by in vitro assays such as 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging activity, 2,2'-azinobis( 3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activity, ferric reducing ability of plasma assay, reducing power, superoxide dismutase-like activity, and iron chelating ability in 3T3-L1 cells. We also measured the total phenol and flavonoids contents (TPC and TFC, respectively) in Trapa japonica shell extract. Our results show that TPC and TFC of Trapa japonica shell extract were 157.7±0.70 mg gallic acid equivalents/g and 25.0±1.95 mg quercetin equivalents/g, respectively. Trapa japonica shell extract showed strong antioxidant activities in a dose-dependent manner in DPPH and ABTS radical scavenging activities and other methods. Especially, the whole antioxidant activity test of Trapa japonica shell extract exhibited higher levels than that of butylated hydroxytoluene as a positive control. Furthermore, Trapa japonica shell extract inhibited lipid accumulation and reactive oxygen species production during the differentiation of 3T3-L1 preadipocytes. Trapa japonica shell extract possessed a significant antioxidant and anti-adipogenic property, which suggests its potential as a natural functional food ingredient.

  7. Synthesis and antioxidant activity of star-shape phenolic antioxidants catalyzed by acidic nanocatalyst based on reduced graphene oxide.

    Science.gov (United States)

    Golestanzadeh, Mohsen; Naeimi, Hossein; Zahraie, Zohreh

    2017-02-01

    Phenolic antioxidants play important role in prevention of oxidation in different industrials. The research objective in the current study was synthesis and evaluate of antioxidant activity of star-shape phenolic antioxidants. The synthetic compounds were prepared in the presence of sulfonated reduced graphene oxide. The antioxidant activity of synthesized compounds was investigated by spectrophotometrically method according to the DPPH assay. Overall, these compounds are potentially important antioxidant and also to limit activity of reactive oxygen species. Copyright © 2016 Elsevier B.V. All rights reserved.

  8. Antioxidant Activity of Potato Peel Extracts in a Fish-RapeseedOil Mixture and in Oil-in-Water Emulsions

    DEFF Research Database (Denmark)

    Farvin, Sabeena; Nielsen, Nina Skall; Jacobsen, Charlotte

    2010-01-01

    The objectives of the present work were (a) to extract the phenolic fraction from the peels of two Danish varieties of potatoes, viz. Sava and Bintje, and examine their antioxidant capacity in in-vitro systems (b) to evaluate the effect of these extracts on the storage stability of a fish- rapeseed...... oil mixture and oil-in-water emulsions. Multiple antioxidant activity of the potato peel extracts was evident from in-vitro systems as they showed strong reducing power, radical scavenging ability, ferrous ion chelating activity and prevented oxidation in a liposome model system. The Sava variety......, which showed strong antioxidant activity in in-vitro systems, was tested in oil and oil-in- water emulsions. Ethanolic extracts of Sava (C1,600 mg/kg) prevented lipid oxidation in emulsions and in oil. Water extracts showed no antioxidant activity in oil whereas it showed pro-oxidant activity...

  9. Antimicrobial and antioxidant activities of two polyketides from lichen-endophytic fungus Preussia sp.

    Science.gov (United States)

    Paudel, Babita; Bhattarai, Keshab; Bhattarai, Hari Datta

    2018-02-23

    Two compounds, compounds 1 and 2, were isolated from Preussia sp. The molecular structures of both compounds were elucidated by analyzing one-dimensional (1D) and 2D nuclear magnetic resonance data along with high-resolution mass spectrometry data. Compound 1 was obtained as novel in structure, and compound 2 was recently reported elsewhere. Compound 1 did not show antioxidant and antimicrobial activities and brine shrimp toxicity, while compound 2 showed strong antioxidant activity (DPPH reduction capacity; IC50=3 μg/mL) and brine shrimp toxicity (LD50=50 μg/mL).

  10. Response of nutrients, minerals, antioxidant leaf pigments, vitamins, polyphenol, flavonoid and antioxidant activity in selected vegetable amaranth under four soil water content.

    Science.gov (United States)

    Sarker, Umakanta; Oba, Shinya

    2018-06-30

    Four selected vegetable amaranths were grown under four soil water content to evaluate their response in nutrients, minerals, antioxidant leaf pigments, vitamins, polyphenol, flavonoid and total antioxidant activity (TAC). Vegetable amaranth was significantly affected by variety, soil water content and variety × soil water content interactions for all the traits studied. Increase in water stress, resulted in significant changes in proximate compositions, minerals (macro and micro), leaf pigments, vitamin, total polyphenol content (TPC), and total flavonoid content (TFC) of vegetable amaranth. Accessions VA14 and VA16 performed better for all the traits studied. Correlation study revealed a strong antioxidant scavenging activity of leaf pigments, ascorbic acid, TPC and TFC. Vegetable amaranth can tolerate soil water stress without compromising the high quality of the final product in terms of nutrients and antioxidant profiles. Therefore, it could be a promising alternative crop in semi-arid and dry areas and also during dry seasons. Copyright © 2018. Published by Elsevier Ltd.

  11. Antioxidant Activity and Hepatoprotective Potential of Polyalthia longifolia and Cassia spectabilis Leaves against Paracetamol-Induced Liver Injury

    Directory of Open Access Journals (Sweden)

    Subramanion L. Jothy

    2012-01-01

    Full Text Available In the present study, in vitro antioxidant, free radical scavenging capacity, and hepatoprotective activity of methanol extracts from Polyalthia longifolia and Cassia spectabilis were evaluated using established in vitro models such as ferric-reducing antioxidant power (FRAP, 2,2-diphenyl-1-picryl-hydrazyl (DPPH•, hydroxyl radical (OH•, nitric oxide radical (NO• scavenging, metal chelating, and antilipidperoxidation activities. Interestingly, all the extracts showed considerable in vitro antioxidant and free radical scavenging activities in a dose-dependent manner when compared to the standard antioxidant which verified the presence of strong antioxidant compound in leaf extracts tested. Phenolic and flavonoid content of these extracts is significantly correlated with antioxidant capacity. Since P. longifolia extract was exhibited better in vitro antioxidant activities, it was subjected for in vivo hepatoprotective activity in paracetamol-intoxicated mice. Therapy of P. longifolia showed the liver protective effect on biochemical and histopathological alterations. Moreover, histological studies also supported the biochemical finding, that is, the maximum improvement in the histoarchitecture of the liver. Results revealed that P. longifolia leaf extract could protect the liver against paracetamol-induced oxidative damage by possibly increasing the antioxidant protection mechanism in mice. Our findings indicated that P. longifolia and C. spectabilis have potential as good sources of natural antioxidant/antiaging compounds.

  12. The TEAC antioxidant activity of 4-hydroxybenzoates

    NARCIS (Netherlands)

    Tyrakowska, B.; Soffers, A.E.M.F.; Szymusiak, H.; Boeren, S.; Boersma, M.G.; Lemanska, J.; Vervoort, J.; Rietjens, I.M.C.M.

    1999-01-01

    The influence of pH, intrinsic electron donating capacity, and intrinsic hydrogen atom donating capacity on the antioxidant potential of series of hydroxy and fluorine substituted 4-hydroxybenzoates was investigated experimentally and also on the basis of computer calculations. The pH-dependent

  13. Proximate, mineral composition, antioxidant activity, and total ...

    African Journals Online (AJOL)

    ... of protein supplement, valuable minerals and dietary antioxidants. They can serve as scavengers of free radicals reported to be associated with occurrence of chronic and degenerative diseases such as cardiovascular diseases. Keywords: Peppers, phytochemicals, Nutritional composition, Free radicals, Diseases ...

  14. Antioxidant activities of ethanolic extracts of four Tunisian olive varieties

    Directory of Open Access Journals (Sweden)

    majda Elkateb

    2015-12-01

    Full Text Available Olive leaves extracts of four olive varieties from Tunisian origin: Chitoui, Chimlali, Sahli and Zarrazi were studied for their phenolic contents, antioxidant activities and their effect on corn oil stability. The amounts of total polyphenolics, flavonoids and proanthocyanidins were determined by spectrophotometry. The antioxidant activities of extracts were evaluated and compared with butylated hydroxytoluene (BHT using a             β-carotene bleaching assay and a 2,2-diphenyl-1-picrylhydrazyl (DPPH radical scavenging method. Chimlali ethanol extract showed the highest total phenolics, flavonoids and proanthocyanidins contents expressed respectively in gallic acid, in quercetin  and catechin equivalent per gram of dried matter 245.5; 9.12 and 40.92. Chimlali ethanol extract showed the highest antioxidant activity coefficient (AAC of 863.636 at 200 mg/ml by the β-carotene bleaching method and the highest scavenging activity of 7.5 µg.mL-1 at 0.1 mg/mL by the DPPH method. The obtained results showed that the variety Chimlali is the richest in phenolic compounds, flavonoids, and proanthocyanidins and  the most potent of antioxidant activity. Analysis of the oxidation results of corn oil, heated to 110°C and during frying, showed a significant antioxidant effect of ethanolic olive leaves extracts relative to the BHT. Olive leaves extracts from varieties Chimlali and Sahli showed the highest antioxidant activity. The synthetic antioxidant BHT exhibits a lower antioxidant power than the extracts from varieties Sahli and Chimlali but is comparable to other varieties. The results suggest the introduction of olive leaves extracts in food preparation, both during storage or heating food, for better preservation.

  15. Effect of Freeze-Drying on the Antioxidant Compounds and Antioxidant Activity of Selected Tropical Fruits

    Directory of Open Access Journals (Sweden)

    Muhammad Redzuan Hairuddin

    2011-07-01

    Full Text Available The effects of freeze-drying on antioxidant compounds and antioxidant activity of five tropical fruits, namely starfruit (Averrhoa carambola L., mango (Mangifera indica L., papaya (Carica papaya L., muskmelon (Cucumis melo L., and watermelon Citruluss lanatus (Thunb. were investigated. Significant (p < 0.05 differences, for the amounts of total phenolic compounds (TPC, were found between the fresh and freeze-dried fruit samples, except muskmelon. There was no significant (p > 0.05 change, however, observed in the ascorbic acid content of the fresh and freeze-dried fruits. Similarly, freeze-drying did not exert any considerable effect on β-carotene concentration of fruits, except for mango and watermelon, where significantly (p < 0.05 higher levels were detected in the fresh samples. The results of DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging and reducing power assays revealed that fresh samples of starfruit and mango had relatively higher antioxidant activity. In case of linoleic acid peroxidation inhibition measurement, a significant (p < 0.05 but random variation was recorded between the fresh and freeze-dried fruits. Overall, in comparison to β-carotene and ascorbic acid, a good correlation was established between the result of TPC and antioxidant assays, indicating that phenolics might have been the dominant compounds contributing towards the antioxidant activity of the fruits tested.

  16. In vitro antioxidant activities of edible artichoke (Cynara scolymus L.) and effect on biomarkers of antioxidants in rats

    DEFF Research Database (Denmark)

    Jimenez-Escrig, A.; Dragsted, Lars Ove; Daneshvar, Bahram

    2003-01-01

    Artichoke (Cynara scolymus L.), an edible vegetable from the Mediterranean area, is a good source of natural antioxidants such as vitamin C, hydroxycinnamic acids, and flavones. The antioxidant activity of aqueous-organic extracts of artichoke were determined using three methods: (a) free radical 2......,2-diphenyl-1-picrylhydrazyl (DPPH.) scavenging, (b) ferric-reducing antioxidant power (FRAP), and...

  17. Comparison in antioxidant and antitumor activities of pine polyphenols and its seven biotransformation extracts by fungi

    OpenAIRE

    Hui Li; Zhenyu Wang

    2017-01-01

    Microbial transformation can strengthen the antioxidant and antitumor activities of polyphenols. Polyphenols contents, antioxidant and antitumor activities of pine polyphenols and its biotransformation extracts by Aspergillus niger, Aspergillus oryzae, Aspergillus carbonarius, Aspergillus candidus, Trichodermas viride, Mucor wutungkiao and Rhizopus sp were studied. Significant differences were noted in antioxidant and antitumor activities. The highest antioxidant activities in Trolox equivale...

  18. Antioxidants

    Science.gov (United States)

    Antioxidants are man-made or natural substances that may prevent or delay some types of cell damage. Antioxidants are found in many foods, including fruits and ... are also available as dietary supplements. Examples of antioxidants include Beta-carotene Lutein Lycopene Selenium Vitamin A ...

  19. Antioxidative and anticholinesterase activity of Cyphomandra betacea fruit.

    Science.gov (United States)

    Ali Hassan, Siti Hawa; Abu Bakar, Mohd Fadzelly

    2013-01-01

    Cyphomandra betacea is one of the underutilized fruits which can be found in tropical and subtropical countries. This study was conducted to determine the antioxidant activity and phytochemical contents in different parts (i.e., flesh and peel) of the fruits. Antioxidants were analyzed using DPPH and ABTS free radical scavenging assays as well as FRAP assay. Anticholinesterase activity was determined using enzymatic assay using acetyl cholinesterase enzyme. For 80% methanol extract, the peel of the fruit displayed higher antioxidant activity in both FRAP and ABTS free radical scavenging assays while the flesh displayed higher antioxidant activity in the DPPH assay. Total phenolic and total flavonoid content were higher in the peel with the values of 4.89 ± 0.04 mg gallic acid equivalent (GAE)/g and 3.36 ± 0.01 mg rutin equivalent (RU)/g, respectively. Total anthocyanin and carotenoid content were higher in the flesh of the fruit with the values of 4.15 ± 0.04 mg/100 g and 25.13 ± 0.35 mg/100 g. The anticholinesterase was also higher in the peel of C. betacea. The same trends of phytochemicals, antioxidant, and anticholinesterase were also observed in the distilled water extracts. These findings suggested that C. betacea has a potential as natural antioxidant-rich nutraceutical products.

  20. Antioxidative and Anticholinesterase Activity of Cyphomandra betacea Fruit

    Directory of Open Access Journals (Sweden)

    Siti Hawa Ali Hassan

    2013-01-01

    Full Text Available Cyphomandra betacea is one of the underutilized fruits which can be found in tropical and subtropical countries. This study was conducted to determine the antioxidant activity and phytochemical contents in different parts (i.e., flesh and peel of the fruits. Antioxidants were analyzed using DPPH and ABTS free radical scavenging assays as well as FRAP assay. Anticholinesterase activity was determined using enzymatic assay using acetyl cholinesterase enzyme. For 80% methanol extract, the peel of the fruit displayed higher antioxidant activity in both FRAP and ABTS free radical scavenging assays while the flesh displayed higher antioxidant activity in the DPPH assay. Total phenolic and total flavonoid content were higher in the peel with the values of 4.89 ± 0.04 mg gallic acid equivalent (GAE/g and 3.36 ± 0.01 mg rutin equivalent (RU/g, respectively. Total anthocyanin and carotenoid content were higher in the flesh of the fruit with the values of 4.15 ± 0.04 mg/100 g and 25.13 ± 0.35 mg/100 g. The anticholinesterase was also higher in the peel of C. betacea. The same trends of phytochemicals, antioxidant, and anticholinesterase were also observed in the distilled water extracts. These findings suggested that C. betacea has a potential as natural antioxidant-rich nutraceutical products.

  1. Analgesic and Antioxidant Activities of Stem Bark Extract and Fractions of Petersianthus macrocarpus

    Science.gov (United States)

    Orabueze, Celestina Ifeoma; Adesegun, Sunday Adeleke; Coker, Herbert Alexander

    2016-01-01

    Background: Petersianthus macrocarpus (Lecythidaceae) is widely used in the folk medicine in Nigeria to relieve pain and fever associated with malaria. This study evaluated the analgesic and antioxidant activities of the methanol extract and fractions of the stem bark of the plant. Materials and Methods: The analgesic activity was determined in mice using hotplate and acetic acid-induced writhing models. Morphine sulphate (5 mg/kg, i.p.) and aspirin (100 mg/ml, p.o.) were used as reference analgesic agents. The antioxidant potential was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical; reducing power, iron chelating properties and determination of total phenolic content. Results: The extract at 200 and 500 mg/kg, produced an insignificant (P > 0.05) increase in pain threshold in hotplate but a significant (P < 0.05) increase at 1000 mg/kg. The extract significantly (P < 0.05) reduced the writhing induced by acetic acid in mice in a dose dependent manner. Fractionation increased the analgesic activities significantly (P < 0.05) in ethyl acetate and aqueous fractions (200 mg/kg). The extract demonstrated strong DPPH radical scavenging activity with IC50 0.05 mg/ml, good reducing power and weak iron chelating activities. The total phenol content was 142.32 mg/gin term of gallic acid. The antioxidant effects were more pronounced in ethyl acetate and aqueous fractions. Conclusion: The findings of the study suggested that the extract has strong analgesic and antioxidant activities which reside mainly in the polar fractions thus confirming the traditional use of the plant to alleviate pains. SUMMARY Analgesic and antioxidant activities of extract and solvent fractions of Petersianthus macrocarpus investigated indicated that extract has analgesic and antioxidant properties that reside mainly in the polar fractions. Abbreviations Used: DMSO: Dimethyl sulphoxide, ANOVA: analysis of variance, EDTA: ethylene diamne tetraacetic acid, SDM: standard deviation of mean

  2. Antioxidant Activity in the Blood and Testes of the Mottled Brown ...

    African Journals Online (AJOL)

    IJAAAR

    homogenate fluids were centrifuged separately to harvest the supernatants and analysed for the antioxidant indices. Statistical comparisons were made between the serum and testicular antioxidant indices. Result showed that the activities of catalase, superoxide dismutase, glutathione peroxidase and total antioxidant.

  3. Polyphenol Content and Antioxidant Activity of Fourteen Wild Edible Fruits from Burkina Faso

    Directory of Open Access Journals (Sweden)

    Odile G. Nacoulma

    2008-03-01

    Full Text Available A total of fourteen (14 species of wild edible fruits from Burkina Faso wereanalyzed for their phenolic and flavonoid contents, and their antioxidant activities usingthe DPPH, FRAP and ABTS methods. The data obtained show that the total phenolic andtotal flavonoid levels were significantly higher in the acetone than in the methanol extracts.Detarium microcarpum fruit had the highest phenolic and the highest flavonoid content,followed by that of Adansonia digitata, Ziziphus mauritiana, Ximenia americana andLannea microcarpa. Significant amounts of total phenolics were also detected in the otherfruit species in the following order of decreasing levels: Tamarindus indica > Sclerocaryabirrea > Dialium guineense > Gardenia erubescens > Diospyros mespiliformis > Parkiabiglobosa > Ficus sycomorus > Vitellaria paradoxa. Detarium microcarpum fruit alsoshowed the highest antioxidant activity using the three antioxidant assays. Fruits with highantioxidant activities were also found to possess high phenolic and flavonoid contents.There was a strong correlation between total phenolic and flavonoid levels and antioxidantactivities.

  4. Antioxidant activities and essential oil composition of Herba Artemisiae Scopariae from China.

    Science.gov (United States)

    Jiang, Shan; Lai, Pengxiang; Li, Jie; Wang, Guichun

    2012-01-01

    The essential oil in traditional Chinese medicine (TCM) Herba Artemisiae Scopariae (HAC) grown in China was obtained by hydrodistillation and studied by GC and GC-MS. Twenty compounds were identified representing 96.6% of the essential oil, of which the most prominent were n-hexadecanoic acid (33.1%), caryophyllene oxide (19.1%) and spathulenol (9.9%). The antioxidant activity of the essential oil (25-400 µg/ml) of HAC was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing/antioxidant power (FRAP) assay. The essential oil of HAC exhibited a strong antioxidant activity, which possess a good potential for use in the food and pharmaceutical industry.

  5. Impact of functional properties and release kinetics on antioxidant activity of biopolymer active films and coatings.

    Science.gov (United States)

    Benbettaïeb, Nasreddine; Tanner, Cadhla; Cayot, Philippe; Karbowiak, Thomas; Debeaufort, Frédéric

    2018-03-01

    This work deals with the study of the release kinetics of some natural antioxidants (ferulic acid, caffeic acid and tyrosol) from chitosan-fish gelatin edible films immersed ethanol at 96%, as well as the kinetics of their antioxidant activity using the DPPH assay. The aim was to determine how film functional properties influence the release kinetic and antioxidant activity. The addition of antioxidants to chitosan-fish gelatin matrix decreased the water vapour permeability by more than 30%. The tensile strength (TS) increased up to 50% after the incorporation of antioxidants. Some molecular interactions between polymer chains and antioxidants were confirmed by FTIR where spectra displayed a shift of the amide-III peak. Films containing caffeic acid or a caffeic-ferulic acid mixture exhibited the highest radical scavenging activity, leading to a 90% antioxidant activity at equilibrium but the release rate controlled the efficacy of the system. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. Antiradical, Chelating and Antioxidant Activities of Hydroxamic Acids and Hydroxyureas

    Directory of Open Access Journals (Sweden)

    Branka Zorc

    2011-07-01

    Full Text Available Reactive oxygen species, along with reactive nitrogen species, may play an important role in the pathogenesis and progress of many diseases, including cancer, diabetes and sickle cell disease. It has been postulated that hydroxyurea, one of the main treatments in sickle cell disease, achieves its activity partly also through its antioxidant properties. A series of hydroxyurea derivatives of L- and D-amino acid amides and cycloalkyl-N-aryl-hydroxamic acids was synthesized and investigated for their radical scavenging activity, chelating properties and antioxidant activity. All the compounds showed exceptional antiradical activities. For example, free radical scavenging activities of investigated hydroxyureas were higher than the activity of standard antioxidant, butylated hydroxyanisole (BHA. Moreover, most of the investigated hydroxamic acids were stronger Fe2+ ion chelators than quercetin. In addition, the investigated compounds, especially hydroxamic acids, were proven to be excellent antioxidants. They were as effective as BHA in inhibiting b-carotene-linoleic acid coupled oxidation. It is reasonable to assume that the antioxidant activity of the investigated compounds could contribute to their previously proven biological properties as cytostatic and antiviral agents.

  7. Biochemical evaluation of antioxidant activity and polysaccharides fractions in seaweeds

    Directory of Open Access Journals (Sweden)

    A. Tariq

    2015-01-01

    Full Text Available In the present study ethanol and water extracts of 15 seaweeds, Dictyota dichotoma var. velutricata, Dictyota indica, Iyengaria stellata, Padina pavonia, Sargassum swartzii, Sargassum variegatum, Stoechospermum marginatum, Stokeyia indica, Jolyna laminarioides, Caulerpa taxifolia, Halimeda tuna, Ulva fasciata, Ulva lactuca, Solieria robusta, and Melanothamnus afaqhusainii, were evaluated for their antioxidant potential by ABTS or 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid, superoxide and total antioxidant capacity (TAC assays.  The activity was concentration dependent and the variation in antioxidant potential was also observed by different assays in both extracts.  Ethanol extract of D. dichotoma var. velutricata, D. indica and S. marginatum demonstrated highest activity by TAC assay.  The antioxidant potential in organic solvent fractions of seaweeds namely P. pavonia, S. swartzii, S. marginatum and M. afaqhusainii was also determined and chloroform fraction of all the four seaweeds showed highest activity by superoxide assay.  Antioxidant activity of extracted fractions of polysaccharides from S. indica, C. taxifolia and D. dichotoma var. velutricata was also evaluated by superoxide method.  Polysaccharide fractions of S. indica obtained from HCl (at 700C and room temperature and water extract demonstrated highest activity respectively.  All the polysaccharide fractions of C. taxifolia showed excellent activity except CaClF70°C. Polysaccharide fractions of D. dichotoma var. velutricata also exhibited very good activity.

  8. Antioxidant Activity of Hawaiian Marine Algae

    OpenAIRE

    Kelman, Dovi; Posner, Ellen Kromkowski; McDermid, Karla J.; Tabandera, Nicole K.; Wright, Patrick R.; Wright, Anthony D.

    2012-01-01

    Marine algae are known to contain a wide variety of bioactive compounds, many of which have commercial applications in pharmaceutical, medical, cosmetic, nutraceutical, food and agricultural industries. Natural antioxidants, found in many algae, are important bioactive compounds that play an important role against various diseases and ageing processes through protection of cells from oxidative damage. In this respect, relatively little is known about the bioactivity of Hawaiian algae that cou...

  9. The Antioxidant Activity and Their Major Antioxidant Compounds from Acanthopanax senticosus and A. koreanum

    Directory of Open Access Journals (Sweden)

    Young-Hyun Kim

    2015-07-01

    Full Text Available The antioxidant activity and chlorogenic acid and caffeic acid contents were investigated from different parts of Acanthopanax senticosus and A. koreanum. Antioxidant activity was assessed by various in vitro assays such as DPPH, ABTS, FRAP, reducing power assays and ORAC, and the chlorogenic acid and caffeic acid were validated by HPLC chromatography. Among the various extracts, the fruit extracts of A. senticosus and A. koreanum exhibited strongest antioxidant activities including ABTS, FRAP, reducing power and ORAC, however, strongest DPPH radical scavenging activity was observed from the leaf extract of A. senticosus. In addition, the antioxidant activities of various extracts were correlated with total phenolic and proanthocyanidin contents. The major phenolic contents from various parts of these plants observed that leaf extract of A. senticosus expressed higher levels of chlorogenic acid (14.86 mg/dry weigh g and caffeic acid (3.09 mg/dry weigh g than other parts. Therefore, these results suggest that the leaf of A. senticosus may be an excellent natural source for functional foods and pharmaceutical agents, and the validated method was useful for the quality control of A. senticosus.

  10. Assay for the antioxidant and radioprotectant activity of extracts form endemic plants

    International Nuclear Information System (INIS)

    Kim, Jin Kyu; Kim, Ji Hyang; Woo, Hyun Jung; Plewa, Michael J.

    2004-01-01

    Since radiation damage and oxygen poisoning occur through the formation of reactive oxygen species, it is a challenging task to develop agents with high antioxidant and radioprotectant activities from plant species. In this study, several species of Korean endemic plants were chosen as experimental candidates. Water-and ethanol extracts were made from the candidates and tested for their antioxidant and radioprotectant activities. In vitro antioxidant assay of the aqueous-organic extracts was carried out using the free radical 2,2-diphenyl-1-picryl-hydrazyl scavenging method. Radioprotective effects were tested by means of experimental on irradiated cell cultures and animals. Among others, the water-extract of Ixeris dentata leaves showed a marked effect on the viability of B16 melanoma cells and provided a radioprotective effect on the number of the leukocytes in the irradiated rodents. DNA damage in the lymphocytes after γ-irradiation decreased in the extract administered animals. Many of the extracts tested in this study showed a slightly lower activity in free radical scavenging than the well-known chemical antiozidants such as ascorbic acid, butylated hydroxytuluene, and glutathione. However, some extracts showed an antioxidant activity similar to that of α-tocopherol acetate and caffeine. These results support the optimistic view for developing radioprotective agents from the Korean endemic plants that showed a strong antioxidant activity

  11. Assay for the antioxidant and radioprotectant activity of extracts form endemic plants

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jin Kyu; Kim, Ji Hyang; Woo, Hyun Jung [KAERI, Taejeon (Korea, Republic of); Plewa, Michael J. [University of Illinois, Illinosi (United States)

    2004-07-01

    Since radiation damage and oxygen poisoning occur through the formation of reactive oxygen species, it is a challenging task to develop agents with high antioxidant and radioprotectant activities from plant species. In this study, several species of Korean endemic plants were chosen as experimental candidates. Water-and ethanol extracts were made from the candidates and tested for their antioxidant and radioprotectant activities. In vitro antioxidant assay of the aqueous-organic extracts was carried out using the free radical 2,2-diphenyl-1-picryl-hydrazyl scavenging method. Radioprotective effects were tested by means of experimental on irradiated cell cultures and animals. Among others, the water-extract of Ixeris dentata leaves showed a marked effect on the viability of B16 melanoma cells and provided a radioprotective effect on the number of the leukocytes in the irradiated rodents. DNA damage in the lymphocytes after {gamma}-irradiation decreased in the extract administered animals. Many of the extracts tested in this study showed a slightly lower activity in free radical scavenging than the well-known chemical antiozidants such as ascorbic acid, butylated hydroxytuluene, and glutathione. However, some extracts showed an antioxidant activity similar to that of {alpha}-tocopherol acetate and caffeine. These results support the optimistic view for developing radioprotective agents from the Korean endemic plants that showed a strong antioxidant activity.

  12. COLOUR, PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF THE SLOVAK ALIBERNET RED WINE SAMPLES

    Directory of Open Access Journals (Sweden)

    Daniel Bajčan

    2015-02-01

    Full Text Available Sixteen Alibernet wine samples, originated from three most important Slovak vineyard regions, were evaluated spectrophotometrically for the content of total polyphenols, content of total anthocyanins, antioxidant activity and wine colour density. The determined total polyphenol contents in observed wines ranged from 2141 to 4274 mg gallic acid per liter (average content was 3057 mg gallic acid/L and total anthocyanin contents ranged from 73.4 to 791.2 mg/L (average content was 403.4 mg/L. Determined values of antioxidant activity were within the interval 54.8 – 86.8% (average value was 74.5% and the values of wine colour density varied between 0.944 and 4.592 (average value was 2.317. The statistical evaluation of the obtained results confirmed only weak linear correlation between total polyphenol content and total anthocyanin content, however strong linear correlations between total anthocyanin content and antioxidant activity, resp. wine colour density were observed. Our results also confirmed very highly significant linear relationship between wine colour density and total polyphenol content, resp. antioxidant activity and between antioxidant activity and total phenolic content.

  13. Gamma irradiation improves the antioxidant activity of Aloe vera (Aloe barbadensis miller) extracts

    International Nuclear Information System (INIS)

    Lee, Eun Mi; Bai, Hyoung-Woo; Lee, Seung Sik; Hong, Sung Hyun; Cho, Jae-Young; Byung, Yeoup Chung

    2012-01-01

    Aloe has been widely used in food products, pharmaceuticals, and cosmetics because of its aromatic and therapeutic properties. In the present study, the ethanolic extracts of aloe gel were gamma-irradiated from 10 to 100 kGy. After gamma irradiation, the color of the ethanolic extracts of aloe gel changed to red; this color persisted up to 40 kGy but disappeared above 50 kGy. Liquid chromatography/mass spectrometry analysis demonstrated the production of a new, unknown compound (m/z=132) after gamma irradiation of the ethanolic extracts of aloe gel. The amount of this unknown compound increased with increasing irradiation up to 80 kGy, and it was degraded at 100 kGy. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by the 1,1-diphenyl-2-picrylhydrazyl-radical scavenging capacity. The antioxidant activity of aloe extract was dramatically increased from 53.9% in the non-irradiated sample to 92.8% in the sample irradiated at 40 kGy. This strong antioxidant activity was retained even at 100 kGy. These results indicate that gamma irradiation of aloe extract can enhance its antioxidant activity through the formation of a new compound. Based on these results, increased antioxidant activity of aloe extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals.

  14. Gamma irradiation improves the antioxidant activity of Aloe vera (Aloe barbadensis miller) extracts

    Science.gov (United States)

    Mi Lee, Eun; Bai, Hyoung-Woo; Sik Lee, Seung; Hyun Hong, Sung; Cho, Jae-Young; Yeoup Chung, Byung

    2012-08-01

    Aloe has been widely used in food products, pharmaceuticals, and cosmetics because of its aromatic and therapeutic properties. In the present study, the ethanolic extracts of aloe gel were gamma-irradiated from 10 to 100 kGy. After gamma irradiation, the color of the ethanolic extracts of aloe gel changed to red; this color persisted up to 40 kGy but disappeared above 50 kGy. Liquid chromatography/mass spectrometry analysis demonstrated the production of a new, unknown compound (m/z=132) after gamma irradiation of the ethanolic extracts of aloe gel. The amount of this unknown compound increased with increasing irradiation up to 80 kGy, and it was degraded at 100 kGy. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by the 1,1-diphenyl-2-picrylhydrazyl-radical scavenging capacity. The antioxidant activity of aloe extract was dramatically increased from 53.9% in the non-irradiated sample to 92.8% in the sample irradiated at 40 kGy. This strong antioxidant activity was retained even at 100 kGy. These results indicate that gamma irradiation of aloe extract can enhance its antioxidant activity through the formation of a new compound. Based on these results, increased antioxidant activity of aloe extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals.

  15. Structural characterization and evaluation of the antioxidant activities of polysaccharides extracted from Qingzhuan brick tea.

    Science.gov (United States)

    Yang, Xinhe; Huang, Mingjun; Qin, Caiqin; Lv, Bangyu; Mao, Qingli; Liu, Zhonghua

    2017-08-01

    The crude tea polysaccharides (CTPS) from Qingzhuan brick tea(QZBT) were extracted and fractionated to afford two fractions, namely TPS-1 and TPS-2. Analyses were conducted concerning the structural characterization and antioxidant activities of these samples. Component analysis revealed that the carbohydrate, uronic acid, protein and polyphenol contents of these samples differed significantly. Fourier transform infrared analysis showed that these samples showed similar characteristic absorption peaks for polysaccharides. Ultraviolet-visible spectroscopy, circular dichroism, scanning electron microscopy and thermogravimetric analyses indicated that there were considerable differences in the presence of protein, surface features, conformational characteristics and thermodynamic behaviors. For antioxidant activities in vitro, CTPS, TPS-1 and TPS-2 exhibited concentration-dependent antioxidant activities, with TPS-2 showing significantly higher antioxidant activity than CTPS and TPS-1. These results provide a scientific and strong foundation for the use of tea polysaccharides(TPS) from QZBT and further research towards the relationships between the characteristics and antioxidant activities of TPS. Copyright © 2017 Elsevier B.V. All rights reserved.

  16. Antioxidant activities of various fruits and vegetables produced in Taiwan.

    Science.gov (United States)

    Huang, Hui-Yu; Chang, Chen-Kang; Tso, Tim K; Huang, Ju-Jen; Chang, Wei-Wei; Tsai, Ying-Chieh

    2004-08-01

    Fruits and vegetables have been known to contain a variety of antioxidant components. It has been suggested that antioxidants may protect biomolecules from oxidative damage and therefore be associated with reduced risks of cardiovascular disease and certain cancer. The antioxidant abilities of various parts of eight common fruits and vegetables produced in Taiwan were investigated, including tomato, guava, squash, tangerine, wax gourd, pineapple, chayote, and eggplant. Squash, wax gourd, tomato, and guava seeds showed the highest antioxidant activities in thiobarbituric acid assay. Wax guard and squash seeds showed the highest antioxidant activities in iodometric assay. At the level of 1 g fresh sample, low-density lipoprotein peroxidation was inhibited by at least 90% by tomato meat, guava meat, squash seed, wax gourd meat, core, and seed, and eggplant skin. The total phenolic content was significantly correlated with antioxidant activities measured by thiobarbituric acid (r=0.715, P<0.01) and iodometric (r=0.749, P<0.01) assays. The results of this study could be used for development of merchandise with potential health benefits from agricultural products.

  17. Antioxidant Activity in Extracts of 27 Indigenous Taiwanese Vegetables

    Directory of Open Access Journals (Sweden)

    Pi-Yu Chao

    2014-05-01

    Full Text Available The objectives of this study were to identify the antioxidants and antioxidant axtivity in 27 of Taiwan’s indigenous vegetables. Lycium chinense (Lc, Lactuca indica (Li, and Perilla ocymoides (Po contained abundant quercetin (Que, while Artemisia lactiflora (Al and Gynura bicolor (Gb were rich in morin and kaempferol, respectively. Additionally, Nymphoides cristata (Nc and Sechium edule (Se-yellow had significantly higher levels of myricetin (Myr than other tested samples. Cyanidin (Cyan and malvidin (Mal were abundant in Gb, Abelmoschus esculentus Moench (Abe, Po, Anisogonium esculentum (Retz. Presl (Ane, Ipomoea batatas (Ib-purple, and Hemerocallis fulva (Hf-bright orange. Relatively high levels of Trolox equivalent antioxidant capacity (TEAC, oxygen radical absorption capacity (ORAC, and 1,1-diphenyl-2-picryl-hydrazyl (DPPH radical scavenger were generated from extracts of Toona sinensis (Ts and Po. Significant and positive correlations between antioxidant activity and polyphenols, anthocyanidins, Que, Myr, and morin were observed, indicating that these phytochemicals were some of the main components responsible for the antioxidant activity of tested plants. The much higher antioxidant activity of Po, Ts, and Ib (purple leaf may be related to their higher Cyan, Que, and polyphenol content.

  18. Antioxidant Activity in Extracts of 27 Indigenous Taiwanese Vegetables

    Science.gov (United States)

    Chao, Pi-Yu; Lin, Su-Yi; Lin, Kuan-Hung; Liu, Yu-Fen; Hsu, Ju-Ing; Yang, Chi-Ming; Lai, Jun-You

    2014-01-01

    The objectives of this study were to identify the antioxidants and antioxidant axtivity in 27 of Taiwan’s indigenous vegetables. Lycium chinense (Lc), Lactuca indica (Li), and Perilla ocymoides (Po) contained abundant quercetin (Que), while Artemisia lactiflora (Al) and Gynura bicolor (Gb) were rich in morin and kaempferol, respectively. Additionally, Nymphoides cristata (Nc) and Sechium edule (Se)-yellow had significantly higher levels of myricetin (Myr) than other tested samples. Cyanidin (Cyan) and malvidin (Mal) were abundant in Gb, Abelmoschus esculentus Moench (Abe), Po, Anisogonium esculentum (Retz.) Presl (Ane), Ipomoea batatas (Ib)-purple, and Hemerocallis fulva (Hf)-bright orange. Relatively high levels of Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorption capacity (ORAC), and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenger were generated from extracts of Toona sinensis (Ts) and Po. Significant and positive correlations between antioxidant activity and polyphenols, anthocyanidins, Que, Myr, and morin were observed, indicating that these phytochemicals were some of the main components responsible for the antioxidant activity of tested plants. The much higher antioxidant activity of Po, Ts, and Ib (purple leaf) may be related to their higher Cyan, Que, and polyphenol content. PMID:24858497

  19. Antioxidant activity in extracts of 27 indigenous Taiwanese vegetables.

    Science.gov (United States)

    Chao, Pi-Yu; Lin, Su-Yi; Lin, Kuan-Hung; Liu, Yu-Fen; Hsu, Ju-Ing; Yang, Chi-Ming; Lai, Jun-You

    2014-05-23

    The objectives of this study were to identify the antioxidants and antioxidant axtivity in 27 of Taiwan's indigenous vegetables. Lycium chinense (Lc), Lactuca indica (Li), and Perilla ocymoides (Po) contained abundant quercetin (Que), while Artemisia lactiflora (Al) and Gynura bicolor (Gb) were rich in morin and kaempferol, respectively. Additionally, Nymphoides cristata (Nc) and Sechium edule (Se)-yellow had significantly higher levels of myricetin (Myr) than other tested samples. Cyanidin (Cyan) and malvidin (Mal) were abundant in Gb, Abelmoschus esculentus Moench (Abe), Po, Anisogonium esculentum (Retz.) Presl (Ane), Ipomoea batatas (Ib)-purple, and Hemerocallis fulva (Hf)-bright orange. Relatively high levels of Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorption capacity (ORAC), and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenger were generated from extracts of Toona sinensis (Ts) and Po. Significant and positive correlations between antioxidant activity and polyphenols, anthocyanidins, Que, Myr, and morin were observed, indicating that these phytochemicals were some of the main components responsible for the antioxidant activity of tested plants. The much higher antioxidant activity of Po, Ts, and Ib (purple leaf) may be related to their higher Cyan, Que, and polyphenol content.

  20. Antioxidant, antibacterial activity, and phytochemical characterization of Melaleuca cajuputi extract.

    Science.gov (United States)

    Al-Abd, Nazeh M; Mohamed Nor, Zurainee; Mansor, Marzida; Azhar, Fadzly; Hasan, M S; Kassim, Mustafa

    2015-10-24

    The threat posed by drug-resistant pathogens has resulted in the increasing momentum in research and development for effective alternative medications. The antioxidant and antibacterial properties of phytochemical extracts makes them attractive alternative complementary medicines. Therefore, this study evaluated the phytochemical constituents of Melaleuca cajuputi flower and leaf (GF and GL, respectively) extracts and their antioxidant and antibacterial activities. Radical scavenging capacity of the extracts was estimated using 2,2-diphenyl-2-picrylhydrazyl and Fe(2+)-chelating activity. Total antioxidant activity was determined using ferric reducing antioxidant power assay. Well diffusion, minimum inhibitory concentration, and minimum bactericidal concentration assays were used to determine antibacterial activity against eight pathogens, namely Staphylococcus aureus, Escherichia coli, Bacillus cereus, Staphylococcus epidermidis, Salmonella typhimurium, Klebsiella pneumonia, Streptococcus pneumoniae, and Pasteurella multocida. We identified and quantified the phytochemical constituents in methanol extracts using liquid chromatography/mass spectrometry (LC/MS) and gas chromatography (GC)/MS. This study reports the antioxidant and radical scavenging activity of M. cajuputi methanolic extracts. The GF extract showed better efficacy than that of the GL extract. The total phenolic contents were higher in the flower extract than they were in the leaf extract (0.55 ± 0.05 and 0.37 ± 0.05 gallic acid equivalent per mg extract dry weight, respectively). As expected, the percentage radical inhibition by GF was higher than that by the GL extract (81 and 75 %, respectively). A similar trend was observed in Fe(2+)-chelating activity and β-carotene bleaching tests. The antibacterial assay of the extracts revealed no inhibition zones with the Gram-negative bacteria tested. However, the extracts demonstrated activity against B. cereus, S. aureus, and S. epidermidis. In

  1. Synthesis, antimicrobial and antioxidative activity of some new isatin derivatives

    Directory of Open Access Journals (Sweden)

    Šekularac Gavrilo M.

    2014-01-01

    Full Text Available The isatin derivatives, Schiff bases, were synthesized by the reaction of isatin and various substituted primary amines and characterized by several spectroscopic methods. Investigation of the antimicrobial activity of the synthesized compounds was performed by the agar dilution method, against different strains of bacteria and one fungi. The antioxidative activity of the synthesized compounds was also determined. Some of the compounds have shown the significant activity against the selected strains of microorganisms and the antioxidative activity. [Projekat Ministarstva nauke Republike Srbije, br. 172013 i III 46010

  2. Antioxidant Activities and Chemical Constituents of Flavonoids from the Flower of Paeonia ostii.

    Science.gov (United States)

    Zhang, Huifang; Li, Xiaofang; Wu, Ke; Wang, Mengke; Liu, Pu; Wang, Xinsheng; Deng, Ruixue

    2016-12-23

    Paeonia ostii is a traditional medicinal plant popularly used in China. This study intended to evaluate the antioxidant properties and the chemical components of the flavonoid-rich extracts from the flowers of P. ostii . The results showed that the flavonoid-rich extracts from the flowers of P. ostii had strong scavenging capacities on 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS), hydroxyls, superoxide anions, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals in a dose-dependent manner. Five flavonoids, dihydrokaempferol ( 1 ), apigenin-7- O -β-d-glucoside ( 2 ), apigenin-7- O -β-d-neohesperidoside ( 3 ), kaempferol-7- O -β-d-glucopyranoside ( 4 ), and kaempferol-3- O -β-d-glucopyranosyl-7- O -β-d-glucopyranoside ( 5 ), were isolated from the flavonoid-rich extracts of the flowers of P. ostii . High-performance liquid chromatography (HPLC) analysis revealed that compounds 3 and 4 were abundant in the P. ostii flower and in flavonoid-rich extracts. The main components of the flower of P. ostii are flavonoids. The high antioxidant activity of the flavonoid-rich extracts may be attributed to the high content of flavonoids. The five isolated flavonoids were the primary antioxidant ingredients, and may play important roles in the strong antioxidant activities of this flower. Based on the obtained results, the flower of P. ostii could be a potential source of natural antioxidants in food and pharmaceutical applications.

  3. Evaluation of Antioxidant and Anti-neuroinflammatory Activities of ...

    African Journals Online (AJOL)

    ... NF-κB activation in LPS-stimulated BV-2 cells. Conclusion: HFE can be considered as a useful therapeutic and preventive approach for the treatment of neurodegenerative diseases and oxidative stress-related diseases. Keywords: Hizikia fusiforme, Antioxidant activity, Anti-neuroinflammation activity, Inducible nitric oxide ...

  4. Antioxidative and antibacterial activity of the methanol extract of ...

    African Journals Online (AJOL)

    Antioxidative and antibacterial activity of the methanol extract of Artemisia anomala S. Moore. ... activity against all five test bacteria and most activity against Gram-negative ones. The minimum inhibitory concentration (MIC) against Proteus vulgaris, Escherichia coli and Salmonella typhi were 1.5, 4.5, 3.9 mg/ml, respectively.

  5. Urolithins display both antioxidant and pro-oxidant activities depending on assay system and conditions.

    Science.gov (United States)

    Kallio, Tuija; Kallio, Johanna; Jaakkola, Mari; Mäki, Marianne; Kilpeläinen, Pekka; Virtanen, Vesa

    2013-11-13

    The biological effects of polyphenolic ellagitannins are mediated by their intestinal metabolites, urolithins. This study investigated redox properties of urolithins A and B using ORAC assay, three cell-based assays, copper-initiated pro-oxidant activity (CIPA) assay, and cyclic voltammetry. Urolithins were strong antioxidants in the ORAC assay, but mostly pro-oxidants in cell-based assays, although urolithin A was an antioxidant in cell culture medium. Parent compound ellagic acid was a strong extracellular antioxidant, but showed no response in the intracellular assay. The CIPA assay confirmed the pro-oxidant activity of ellagitannin metabolites. In the cell proliferation assay, urolithins but not ellagic acid decreased growth and metabolism of HepG2 liver cells. In cyclic voltammetry, the oxidation of urolithin A was partly reversible, but that of urolithin B was irreversible. These results illustrate how strongly measured redox properties depend on the employed assay system and conditions and emphasize the importance of studying pro-oxidant and antioxidant activities in parallel.

  6. Antioxidant activity relationship of phenolic compounds in Hypericum perforatum L.

    Directory of Open Access Journals (Sweden)

    Petrović Slobodan S

    2011-06-01

    Full Text Available Abstract Background The St John's Wort (Hypericum perforatum; Clusiaceae has been used in traditional and modern medicine for a long time due to its high content of biologically active phenolics. The purpose of this work was to develop a method for their fractionation and identification, and to determine the most active antioxidant compounds in plant extract. Results An LC-MS method which enables fast qualitative and semiquantitative analysis was developed. The composition determined is in agreement with the previous results, where 6 flavonoids, 4 naphthodianthrones and 4 phloroglucinols have been identified. Significant antioxidant activity was determined for most of the fractions by DPPH assay (the lowest IC50 of 0.52 μg/ml, NO scavenging (6.11 μg/ml, superoxide scavenging (1.86 μg/ml, lipid peroxidation (0.0079 μg/ml and FRAP (the highest reduction capacity of 104 mg Fe equivalents/g assays. Conclusion LC-MS technique has been successfully applied for a quick separation and identification of the major components of H. perforatum fractions. Majority of the fractions analyzed have expressed a very high antioxidative activity when compared to synthetic antioxidants. The antioxidant activity could be attributed to flavonoids and phenolic acids, while phloroglucinols and naphthodianthrones showed no significant activity. It is demonstrated that it is possible to obtain, by fractionation, H. perforatum preparations with significantly increased phloroglucinols-to-naphthodianthrones ratio (up to 95:5.

  7. Study on the antioxidant and antitumoral activity of propolis

    Directory of Open Access Journals (Sweden)

    Elisa del Río del Rosal

    Full Text Available ABSTRACT Introduction: Propolis is the substance that protects the hive, a resin of complex and viscous composition bees use in the repair and protection of the hive. The material from which propolis arises are the resins, shoots and petioles of the leaves of different plants, so it has a very complex chemical composition that varies depending on the flora of the bees collection. It offers an antimicrobial, anti-inflammatory capacity related to its antioxidant, immunomodulatory power, among others. Aims: In this work, antioxidant and antitumoral activities of different propolis collected from different areas of the province of Malaga, comparing them with one from the Bohemian region to the south of the Czech Republic are studied. Material and methods: Antioxidant activity was determined according to the ABTS+/S2O8K2 method. In addition, the quantity of total proteins from the nitrogen content and subsequently the cytotoxicity and antitumoral activity of the propolis of Puerto de la Torre, north of Malaga, are measured according to the 3-(4,5-dimetiltiazol-2-il-2,5-diphenyl tetrazolium bromide method. Results: It was observed that propolis has high antioxidant activity, although it has a lower amount of proteins. Propolis has high toxicity and higher antitumoral activity against colon cancer than leukemia. Discussion: With all these data, it can be concluded that propolis offers different activities of interest, for the food and cosmetic industry, among which the high antioxidant and antitumoral capacity.

  8. Antioxidant activity and phenylpropanoids of Phlomis lychnitis L

    DEFF Research Database (Denmark)

    López, Víctor; Jäger, Anna Katharina; Akerreta, Silvia

    2010-01-01

    Phlomis lychnitis L. (Lamiaceae) is consumed as a traditional herbal tea in Spain. The antioxidant-protective effects as well as its phytoconstituents have never been established. The ability of the methanolic extract to protect cells from oxidative stress was evaluated in rat pheochromocytoma...... cells (PC12) using hydrogen peroxide as toxic agent. The viability of PC12 cells pre-treated with the methanolic extract of Phlomis lychnitis, determined by the MTT and LDH assays, was significantly improved at the highest dose (p antioxidant activity was confirmed evaluating the capacity...... of the plant to scavenge ABTS, DPPH, O(2) . (-) radicals and to inhibit XO. Bioassay guided fractionation led to antioxidant compounds. Qualitative HPLC/DAD/ESI/MS analysis reported phenylpropanoids, verbascoside being the major antioxidant constituent....

  9. Phytochemicals Screening and Activities of Hydrophilic and Lipophilic Antioxidant of Some Fruit Peels

    International Nuclear Information System (INIS)

    Khairusy Syakirah Zulkifli; Noriham Abdullah; Aminah Abdullah; Nurain Aziman; Wan Saidatul Syida Wan Kamarudin

    2012-01-01

    This study was conducted to screen the secondary metabolites compounds including alkaloids, tannins, saponins and flavonoids as well as to determine the antioxidant activities of four types of fruit peels namely Psidium guajava (guava), Mangifera indica (Chakonan mango), Citrus sinensis (Navel orange) and Malus sylvestris (Granny Smith apple). The hydrophilic and lipophilic antioxidant activities were investigated using three different assays such as Ferric Reducing Antioxidant Power (FRAP), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and Oxygen Radical Absorbance Capacity (ORAC). Total Phenolic Content (TPC) and Total Flavonoids Content (TFC) were also conducted and the correlations between the antioxidant assays with TPC and TFC were evaluated. The TPC in the peels extract ranged between 204.90 to 517.00 mg GAE/ g extract weight while TFC ranged between 97.48 to 177.86 mg QE/ g extract weight. The FRAP, EC 50 scavenging activity and ORAC values were 18.78 to 45.36 mM TE/ 100 g extract weight, 0.146 to 0.717 mg/ ml scavenging effect and 37.54 to 60.59 μM TE/ g extract weight respectively. The extract of M. indica peels appeared to be as potent as ascorbic acid with maximum inhibition of 74 % at 200 ppm. M. indica peels showed highest value in all antioxidant assays and in TPC while the highest in TFC was found in M. sylvestris peels. There were strong correlations between all antioxidant assays with TPC but very weak correlations with TFC. This study suggested that the extracts of fruit peels are convenient to be use as functional ingredients in food product development as they are rich in antioxidant activities. (author)

  10. Enhancing antioxidant activity of sesamol at frying temperature by addition of additives through reducing volatility.

    Science.gov (United States)

    Hwang, Hong-Sik; Winkler-Moser, Jill K; Vermillion, Karl; Liu, Sean X

    2014-11-01

    Additives were evaluated to investigate their effects on volatility of sesamol at frying temperature with the hypothesis that the interaction between an additive and sesamol would reduce sesamol volatility. Twenty-two additive : sesamol combinations were examined by thermogravimetric analysis (TGA) under nitrogen in neat form and in soybean oil. The results indicate that these additives could bind to or interact with sesamol and consequently reduced its volatility. (1) H NMR study provided evidence for hydrogen bonding between sesamol and a hydroxyl group, an amino group, and ether groups. Subsequent heating tests were conducted to investigate the effect of the reduced volatility of sesamol on antioxidant activity in soybean oil at 180 °C. Oxidation of soybean oil was monitored with gel permeation chromatography for formation of polymerized triacylglycerols and with (1) H NMR for loss of olefinic and bisallylic protons. Sesamol retained in soybean oil during the heating process was determined by high-performance liquid chromatography. A strong correlation between the retained sesamol and the antioxidant activity was observed. The mixture of 830 ppm sesamol and mono-/diglycerides, polysorbate 20 or l-carnosine showed much improved antioxidant activity compared to sesamol itself and slightly better antioxidant activity than 200 ppm tert-butylhydroquinone. It is believed that this method can also be used for many other antioxidants for which volatility is a problem. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.

  11. The antimicrobial activity of Liquidambar orientalis mill. against food pathogens and antioxidant capacity of leaf extracts.

    Science.gov (United States)

    Okmen, G; Turkcan, O; Ceylan, O; Gork, G

    2014-01-01

    Medicinal plants are an important source of substances which are claimed to induce antimicrobial, antimutagenic and antioxidant effects. Many plants have been used due to their antimicrobial treatments. Antimicrobial and antioxidant activities of L. orientalis have not been reported to the present day. The aim of this work was to investigate of the antimicrobial and antioxidant potentials of different extracts from L. orientalis. The extracts were screened for antimicrobial activity against different food pathogens. These bacteria include 4 Gram positive and 3 Gram negative bacteria and one fungi. The leaf extracts of plant were tested by disc diffusion assay. The MIC was evaluated on plant extracts as antimicrobial activity. In addition to, the plant extracts were tested against the stable DPPH (2,2-diphenyl-1-picryl-hydrazylhydrate) free-radical. The acetone, ethanol and methanol extracts of L. orientalis showed maximum inhibition zone of 12 mm against Yersinia enterocolitica, Listeria monocytogenes and Staphylococcus aureus. In addition to, the methanol extract displayed a strong antioxidant activity (trolox equivalent = 2.23 mM). L. orientalis extracts have antimicrobial, and antioxidant potential. Our results support the use of this plant in traditional medicine and suggest that some of the plant extracts possess compounds with good antibacterial properties that can be used as antibacterial agents in the search for new drugs.

  12. Antioxidant and Antimicrobial Activities of Quinoa (Chenopodium quinoa Willd.) Seeds Cultivated in Korea

    Science.gov (United States)

    Park, Jin Hwa; Lee, Yun Jin; Kim, Yeon Ho; Yoon, Ki Sun

    2017-01-01

    The objective of this study was to investigate the antioxidant and antimicrobial properties of quinoa cultivated in Korea and to compare it with imported quinoa from the USA and Peru. The highest amount of total flavonoid contents (TFC) with 20.91 mg quercetin equivalents/100 g was measured in quinoa seed extract cultivated in Korea, while the total phenolic contents (TPC) were significantly higher in quinoa from the USA (16.28 mg gallic acid equivalents/100 g). In addition, quinoa extracts cultivated in Korea displayed a superior antioxidant ability in both, ferric reducing antioxidant power and 1,1-diphenyl-2-picrylhydrazyl values. There was a high correlation between TFC and antioxidant activity and a low correlation between TPC and antioxidant activity. The antimicrobial activity of the quinoa extracts was determined using a disc diffusion assay and optical density method. In both assays, the quinoa seed extracts did not have strong antimicrobial activity against foodborne bacteria, including Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, Escherichia coli, Salmonella Typhimurium, and Campylobacter jejuni. PMID:29043217

  13. Antioxidant and Antimicrobial Activities of Quinoa (Chenopodium quinoa Willd.) Seeds Cultivated in Korea.

    Science.gov (United States)

    Park, Jin Hwa; Lee, Yun Jin; Kim, Yeon Ho; Yoon, Ki Sun

    2017-09-01

    The objective of this study was to investigate the antioxidant and antimicrobial properties of quinoa cultivated in Korea and to compare it with imported quinoa from the USA and Peru. The highest amount of total flavonoid contents (TFC) with 20.91 mg quercetin equivalents/100 g was measured in quinoa seed extract cultivated in Korea, while the total phenolic contents (TPC) were significantly higher in quinoa from the USA (16.28 mg gallic acid equivalents/100 g). In addition, quinoa extracts cultivated in Korea displayed a superior antioxidant ability in both, ferric reducing antioxidant power and 1,1-diphenyl-2-picrylhydrazyl values. There was a high correlation between TFC and antioxidant activity and a low correlation between TPC and antioxidant activity. The antimicrobial activity of the quinoa extracts was determined using a disc diffusion assay and optical density method. In both assays, the quinoa seed extracts did not have strong antimicrobial activity against foodborne bacteria, including Staphylococcus aureus , Listeria monocytogenes , Bacillus cereus , Escherichia coli , Salmonella Typhimurium, and Campylobacter jejuni .

  14. Alkyl caffeates improve the antioxidant activity, antitumor property and oxidation stability of edible oil.

    Directory of Open Access Journals (Sweden)

    Jun Wang

    Full Text Available Caffeic acid (CA is distributed widely in nature and possesses strong antioxidant activity. However, CA has lower solubility in non-polar media, which limits its application in fat-soluble food. To increase the lipophilicity of natural antioxidant CA, a series of alkyl caffeates were synthesized and their antioxidant and antitumor activities were investigated. The antioxidant parameters, including the induction period, acid value and unsaturated fatty acid content, of the alkyl caffeates in edible oil were firstly investigated. The results indicated that alkyl caffeates had a lower DPPH IC₅₀ (14-23 µM compared to CA, dibutyl hydroxy toluene (BHT and Vitamin C (24-51 µM, and significantly inhibited four human cancer cells (SW620, SW480, SGC7901 and HepG2 with inhibition ratio of 71.4-78.0% by a MTT assay. With regard to the induction period and acid value assays, methyl and butyl caffeates had higher abilities than BHT to restrain the oxidation process and improve the stability of edible oil. The addition of ethyl caffeate to oil allowed maintenance of a higher unsaturated fatty acid methyl ester content (68.53% at high temperatures. Overall, the alkyl caffeats with short chain length (n<5 assessed better oxidative stability than those with long chain length. To date, this is the first report to the correlations among the antioxidant activity, anticancer activity and oxidative stability of alkyl caffeates.

  15. Antioxidant activity of Egyptian Eucalyptus camaldulensis var. brevirostris leaf extracts.

    Science.gov (United States)

    El-Ghorab, Ahmed H; El-Massry, Khaled F; Marx, Friedhelm; Fadel, Hoda M

    2003-02-01

    Leaves from Eucalvptus camaldulensis var. brevirostris trees, planted in the Nile delta in Egypt, were examined for the antioxidant activity of their nonvolatile compounds. The extracts obtained by ethanol digestion and by supercritical fluid extraction (SFE; CO2 with 15% ethanol) showed the most promising antioxidative activities. In order to identify the most active compounds, both extracts were subjected to a semipreparative reversed-phase HPLC separation, the main fractions were collected, tested for antioxidative activity and analysed by different chromatographical and spectroscopical methods for identification of the most relevant compounds. Gallic and ellagic acid were found to be the prevailing antioxidants in the ethanolic extract. The main two compounds of the SFE extract with antioxidative activity revealed to be flavones. To a high degree of probability they were identified as 5-hydroxy-7,4'-dimethoxy flavone and 5-hydroxy-7,4'-dimethoxy-8-methyl flavone, respectively. The extracts obtained by ethanoldigestion were dried and administered to rats for toxicity evaluation (up to 3 g/kg body weight). No mortality was observed which indicates a very low lethality of the tested extract.

  16. Spectroscopic studies on the antioxidant activity of ellagic acid

    Science.gov (United States)

    Kilic, Ismail; Yeşiloğlu, Yeşim; Bayrak, Yüksel

    2014-09-01

    Ellagic acid (EA, C14H6O8) is a natural dietary polyphenol whose benefits in a variety of diseases shown in epidemiological and experimental studies involve anti-inflammation, anti-proliferation, anti-angiogenesis, anticarcinogenesis and anti-oxidation properties. In vitro radical scavenging and antioxidant capacity of EA were clarified using different analytical methodologies such as total antioxidant activity determination by ferric thiocyanate, hydrogen peroxide scavenging, 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, 2,2‧-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity and superoxide anion radical scavenging, ferrous ions (Fe2+) chelating activity and ferric ions (Fe3+) reducing ability. EA inhibited 71.2% lipid peroxidation of a linoleic acid emulsion at 45 μg/mL concentration. On the other hand, butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), α-tocopherol and ascorbic acid displayed 69.8%, 66.8%, 64.5% and 59.7% inhibition on the peroxidation of linoleic acid emulsion at the same concentration, respectively. In addition, EA had an effective DPPH• scavenging, ABTSrad + scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, ferric ions (Fe3+) reducing power and ferrous ions (Fe2+) chelating activities. Also, those various antioxidant activities were compared to BHA, BHT, α-tocopherol and ascorbic acid as references antioxidant compounds. These results suggested that EA can be used in the pharmacological, food industry and medicine because of these properties.

  17. The antioxidant activity of selected wild orchids of Nepal

    Directory of Open Access Journals (Sweden)

    Mukesh Babu Chand

    2016-09-01

    Full Text Available Objective: To assess the antioxidant activity as well as the total polyphenolics and flavonoids content of thirteen extracts from nine wild orchids of Nepal. Methods: First, a preliminary phytochemical screening of thirteen extracts of nine orchids was carried out using established protocols. Then, the total polyphenolic and flavonoid contents of each extract were evaluated using the Folin-Ciocalteu's phenol reagent and aluminium chloride methods respectively. Finally, antioxidant activity was determined by 2, 2-diphenyl-1-picryl hydrazyl free radical scavenging assay and expressed in terms of half inhibition concentrations (IC50 values. Results: All the tested parameters showed significant variation at P = 0.05. The total flavonoids varied with the greatest amounts found in the leaves of Rhynchostylis retusa and the smallest amounts found in the roots of Gastrochilus acutifolius (G. acutifolius. The amount of total polyphenolics was highest in the stems of Vanda cristata and lowest in the leaves of G. acutifolius. Antioxidant activity was highest in the stems of Vanda cristata and lowest in the leaves of G. acutifolius. Conclusions: Some of the orchid extracts studied exhibited levels of antioxidant activity which were comparable to or even higher than those found for extracts of medicinal plants and orchids studied previously. It is suggested that drugs and natural food additives can be extracted from those wild orchids with high levels of antioxidant activity.

  18. Relationship between Antioxidant and Anticancer Activity of Trihydroxyflavones

    Directory of Open Access Journals (Sweden)

    Ignas Grigalius

    2017-12-01

    Full Text Available Plant polyphenols have been highlighted not only as chemopreventive, but also as potential anticancer substances. Flavones are a subclass of natural flavonoids reported to have an antioxidant and anticancer activity. The aim of our study was to evaluate antioxidant and anticancer activity of seventeen trihydroxyflavone derivatives, including apigenin (API and baicalein (BCL. Also, we wanted to find out if there is a correlation between those two effects. Cell growth inhibition testing was carried out using MTT assay in three different human cancer cell lines: lung (A549, breast (MCF-7 and brain epithelial (U87. Antioxidant activity was determined by the DPPH radical scavenging method. Thirteen trihydroxyflavones possessed anticancer activity against at least one tested cancer cell line. They were more active against the MCF-7 cell line, and the lowest activity was determined against the U87 cell line. The majority of compounds inhibited cancer cell growth at EC50 values between 10–50 µM. The most active compound was 3’,4’,5-trihydroxyflavone 7, especially against A549 and MCF-7 cell lines. The correlation between anti-proliferative and antioxidant activity was only moderate, and it was determined for A549 and U87 cancer cell lines. The most important fragment for those two effects is the ortho-dihydroxy group in ring B. Conclusions. Trihydroxyflavones demonstrated anticancer activity. Further and more detailed studies should to be carried out to estimate the structure–activity relationship of these compounds.

  19. In Vivo Antioxidant Activity of Deacetylasperulosidic Acid in Noni

    Directory of Open Access Journals (Sweden)

    De-Lu Ma

    2013-01-01

    Full Text Available Deacetylasperulosidic acid (DAA is a major phytochemical constituent of Morinda citrifolia (noni fruit. Noni juice has demonstrated antioxidant activity in vivo and in human trials. To evaluate the role of DAA in this antioxidant activity, Wistar rats were fed 0 (control group, 15, 30, or 60 mg/kg body weight per day for 7 days. Afterwards, serum malondialdehyde concentration and superoxide dismutase and glutathione peroxidase activities were measured and compared among groups. A dose-dependent reduction in malondialdehyde was evident as well as a dose-dependent increase in superoxide dismutase activity. DAA ingestion did not influence serum glutathione peroxidase activity. These results suggest that DAA contributes to the antioxidant activity of noni juice by increasing superoxide dismutase activity. The fact that malondialdehyde concentrations declined with increased DAA dose, despite the lack of glutathione peroxidase-inducing activity, suggests that DAA may also increase catalase activity. It has been previously reported that noni juice increases catalase activity in vivo but additional research is required to confirm the effect of DAA on catalase. Even so, the current findings do explain a possible mechanism of action for the antioxidant properties of noni juice that have been observed in human clinical trials.

  20. Studies on the antioxidant activity of Echinacea root extract.

    Science.gov (United States)

    Hu, C; Kitts, D D

    2000-05-01

    Methanol extracts of freeze-dried Echinacea (E. angustifolia, E. pallida, and E. purpurea) roots were examined for free radical scavenging capacities and antioxidant activities. Root extracts of E. angustifolia, E. pallida, and E. purpurea were capable of scavenging hydroxyl radical. Similar scavenging activities for each variety were found for both 1,1-diphenyl-2-picrylhydrazyl radical and ABTS radical. Meanwhile, antioxidant activities of all three varieties of Echinacea were found to delay the formation of conjugated diene hydroperoxide induced by the thermal decomposition of 2, 2'-azobis(2-amidinopropane) dihydrochloride and extend the lag phase of peroxidation of soybean liposomes. Echinacea root extracts suppressed the oxidation of human low-density lipoprotein, as evaluated by reduced agarose electrophoretic mobility following oxidative modification by Cu(2+). The mechanisms of antioxidant activity of extracts derived from Echinacea roots included free radical scavenging and transition metal chelating.

  1. In vitro and in vivo antioxidant activity of a fructan from the roots of Arctium lappa L.

    Science.gov (United States)

    Liu, Wei; Wang, Jiajia; Zhang, Zhenzhen; Xu, Jinnan; Xie, Zhuohong; Slavin, Margaret; Gao, Xiangdong

    2014-04-01

    To explore new antioxidant resource from food, a water-soluble polysaccharide (ALP1) was extracted and purified from the roots of Arctium lappa L. (A. lappa L.) through hot water extraction followed by ethanol precipitation, ion-exchange chromatography and gel filtration. The antioxidant activity of ALP1 was then evaluated in vitro and in vivo. ALP1 was characterized as a fructan composed of fructose and glucose in the ratio of 13.0:1.0, with an average molecular weight of 4600 Da. The linkages in ALP1 were →1)-Fruf-(2→, Fruf-(2→ and Glcp-(1→. In vitro antioxidant assays demonstrated that ALP1 possessed moderate ABTS(+) scavenging activity, strong hydroxyl radical scavenging activity and strong ferrous ion chelating activity. In in vivo antioxidant assays, ALP1 administration significantly enhanced antioxidant enzyme activities and total antioxidant capacity, as well as decreased the levels of malondialdehyde (MDA) in both the serum and liver of aging mice. These results suggest that ALP1 has potential as a novel natural antioxidant in food industry and pharmaceuticals. Copyright © 2014 Elsevier B.V. All rights reserved.

  2. pH dependent antioxidant activity of lettuce (L. sativa) and synergism with added phenolic antioxidants.

    Science.gov (United States)

    Altunkaya, Arzu; Gökmen, Vural; Skibsted, Leif H

    2016-01-01

    Influence of pH on the antioxidant activities of combinations of lettuce extract (LE) with quercetin (QC), green tea extract (GTE) or grape seed extract (GSE) was investigated for both reduction of Fremy's salt in aqueous solution using direct electron spin resonance (ESR) spectroscopy and in L-α-phosphatidylcholine liposome peroxidation assay measured following formation of conjugated dienes. All examined phenolic antioxidants showed increasing radical scavenging effect with increasing pH values by using both methods. QC, GTE and GSE acted synergistically in combination with LE against oxidation of peroxidating liposomes and with QC showing the largest effect. The pH dependent increase of the antioxidant activity of the phenols is due to an increase of their electron-donating ability upon deprotonation and to their stabilization in alkaline solutions leading to polymerization reaction. Such polymerization reactions of polyphenolic antioxidants can form new oxidizable -OH moieties in their polymeric products resulting in a higher radical scavenging activity. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract

    Directory of Open Access Journals (Sweden)

    Neelesh Sharma

    2014-09-01

    Full Text Available This study evaluated the chemical composition, antioxidant, anti-inflammatory and anticancer activities of a Euphorbia hirta L. extract. The antioxidant activities of whole E. hirta ethanol extract were determined by electron spin resonance spectrophotometric analysis of 1,1-diphenyl-2-picryl-hydrazyl (DPPH, hydroxyl, and alkyl radical levels and by using an online high-performance liquid chromatography (HPLC-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid assay. The E. hirta ethanol extract (0.5 mg/mL exhibited DPPH-scavenging activity of 61.19% ± 0.22%, while the positive control (0.5 mg/mL ascorbic acid had 100% ± 0.22% activity. The concentration of the extract required to trap 50% of DPPH (IC50 was 0.205 mg/mL. Online HPLC analysis of the extract also showed strong antioxidant activity. The anti-inflammatory activity of the E. hirta extract was assessed in lipopolysaccharide-induced RAW 264.7 macrophages. The anti-inflammatory activity was highest in the presence of 200 µg/mL E. hirta extract, and nitric oxide production was decreased significantly (p < 0.05. The extract also showed selective anticancer activity at a concentration of 100 µg/mL (p < 0.05. These results indicated that E. hirta may warrant further investigation for the development of antioxidant, anti-inflammatory, and anticancer herbal medications.

  4. Flavonoid Glycosides from Siparuna gigantotepala Leaves and Their Antioxidant Activity.

    Science.gov (United States)

    Torres Castañeda, Harlen Gerardo; Colmenares Dulcey, Ana Julia; Isaza Martínez, José Hipólito

    2016-01-01

    Two new flavonol glycosides were isolated from the leaves of Siparuna gigantotepala. Their structures were determined to be kaempferol 3-O-β-xylopyranosyl-(1→2)-α-arabinofuranoside (1) and kaempferol 3,7-di-O-methyl-4'-O-α-rhamnopyranosyl-(1→2)-β-glucopyranoside (2). In addition, three known flavonol glycosides, rutin (3), kaempferol 3-O-rutinoside (4), and kaempferol 3,7-di-O-methyl-4'-O-rutinoside (5), and three flavonol aglycones, quercetin (6), kaempferol 3,7-dimethyl ether (7), and kaempferol 3,7,4'-trimethyl ether (8), were also isolated and are reported here for the first time in this species. The structures of compounds 1 and 2 were established on the basis of their LC-MS and one- and two-dimensional (1D)- and (2D)-NMR spectroscopic analyses, combined with acid methanolysis and silylation of sugar moieties for GC-MS. Evaluation of the antioxidant activity, conducted in the 96-well plate format, showed that the flavonoids isolated possess strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and moderate oxygen radical absorption capacity.

  5. Antiproliferation effects and antioxidant activity of two new Lactobacillus strains

    Directory of Open Access Journals (Sweden)

    Parisa Shokryazdan

    2017-11-01

    Full Text Available Abstract The microorganisms most commonly used as probiotics are lactic acid bacteria, especially those of the genus Lactobacillus. In the present study, two Lactobacillus strains, L. pentosus ITA23 and L. acidipiscis ITA44, previously isolated from mulberry silage, were characterized for their antiproliferative and antioxidant activities. The antiproliferative effects of the strains were investigated using the MTT assay with breast cancer (MDA-MB-231, liver cancer (HepG2 and normal liver (Chang cell lines. The strains were tested for their antioxidant activity using the FRAP and ABTS methods. The results showed that the two Lactobacillus strains had good antiproliferative effects against both cancer cell lines tested, while their effects on the normal cells were weak. Based on the results of the antioxidant tests, the intact cells and cell-free extracts of the two Lactobacillus strains showed more than 135 and less than 50 µg trolox/ml of antioxidant activity, respectively. Lactobacillus pentosus ITA23 and L. acidipiscis ITA44 can be considered as potential probiotic candidates for humans because of their antioxidant activity and antiproliferation effects against cancer cells.

  6. Biosynthesis of silver nanoparticles from Catharanthus roseus leaf extract and assessing their antioxidant, antimicrobial, and wound-healing activities.

    Science.gov (United States)

    Al-Shmgani, Hanady S A; Mohammed, Wasnaa H; Sulaiman, Ghassan M; Saadoon, Ali H

    2017-09-01

    Biosynthesis of silver nanoparticles (AgNPs) from Catharanthus roseus leaf extract was carried out, and their characterization, as well as antioxidant, antimicrobial, and wound-healing activities were evaluated. Color change, UV-vis spectrum, XRD, FTIR, and AFM assessments supported the biosynthesis and characterization of AgNPs. The synthesized AgNPs showed strong in vitro antioxidant and antimicrobial activities against various pathogens. The in vivo assessment of wound healing in AgNPs-treated mice revealed their effectiveness in closuring and reducing size of wounds. Such potent bioactivity may justify their biomedical use as antioxidant and antimicrobial agents for controlling various health-related diseases, particularly in wound healing.

  7. Antioxidant Activities of Three Rubus Species Growing in Ethiopia ...

    African Journals Online (AJOL)

    80% methanol extract, and acetone and methanol soxhlet fractions of the three plants tested showed antioxidant activity in a concentration-dependent manner. The 80% methanolic extract of R. steudneri showed the strongest activity in the DPPH radical scavenging assay (IC50 = 6.5 mg/ml). In the deoxyribose degradation ...

  8. Antioxidant activity of Piper caninum and Cyclooxygenase-2 ...

    African Journals Online (AJOL)

    Background: This study investigated on antioxidant activity of Piper caninum and cyclooxygenase-2 inhibition by methoxylated flavones. Materials and methods: The present study was carried out to quantify the total phenolic content and free radical scavenging activities of the crude extracts by Folin-Ciocalteu and 1 ...

  9. In vitro Antifungal, Antioxidant and Cytotoxic Activities of a Partially ...

    African Journals Online (AJOL)

    higher antifungal activity on Candida albicans than on Aspergillus fumigatus. AMP III fraction showed greater in vitro antioxidant activity than the aqueous extract. SDS-PAGE analyses revealed the presence of two protein bands with molecular weight approximately of 16 and 67 KDa in AMP III. Protein concentration was 240 ...

  10. Antioxidant Activities of Methanol Extract and Solvent Fractions of ...

    African Journals Online (AJOL)

    2015-02-09

    Feb 9, 2015 ... and weak blood anticoagulant activity [12] detected in A. erecta extracts, no other bioactivities have been reported for the species. Extracts and compounds isolated from other. Avrainvillea species have been found to exhibit antioxidant activity [10]. It is therefore likely that. A. erecta may also contain potent ...

  11. Comparative profiling of biomarker psoralen in antioxidant active ...

    African Journals Online (AJOL)

    Comparative profiling of biomarker psoralen in antioxidant active extracts of different species of genus Ficus by validated HPTLC method. ... an important tool to assure the therapeutic dose of active ingredients in herbal formulations as well as for standardization and quality control of bulk drugs and in-process formulations.

  12. Antioxidant activity of acetone and ethanolic leaves extracts of ...

    African Journals Online (AJOL)

    The measured antioxidant properties included free radical scavenging activities against 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azinobis-3- ethylbenzothizoline 6-sulfonic acid (ABTS), ferric reducing power activities, hydrogen peroxide, nitric oxide scavenging properties and lipid peroxidation. The results show that the ...

  13. Characterization and Antioxidant Activity of the Complex of ...

    African Journals Online (AJOL)

    infrared spectrometry (IR), differential scanning calorimetry (DSC) and x-ray diffractometry (XRD). The antioxidant activity was examined by DPPH and ABTS radical-scavenging activities. Results: Phloridzin in the complex was molecularly dispersed in HP-β-CD matrix. The complex was an effective scavenger of DPPH and ...

  14. Antimicrobial and antioxidant activities of Momordica charantia from ...

    African Journals Online (AJOL)

    In the present study, unripe/ripe seed and fruit ethanol extracts of M. charantia from Turkey were screened for their potential antimicrobial and antioxidant activities. The antimicrobial activities of the extract were determined against four gram positive bacteria, seven gram negative bacteria, and one yeast with disc diffusion ...

  15. Antioxidant activity in some selected Indian medicinal plants | Kumar ...

    African Journals Online (AJOL)

    The study was carried out to determine the antioxidant activity of selected medicinal plants namely Albizia amara, Achyranthes aspera, Cassia fistula, Cassia auriculata and Datura stramonium by inhibition of lipid peroxidation technique. The highest inhibition of lipid peroxidation activity was observed in A. amara (96%) ...

  16. Comparative study of in vitro antioxidant activity of foliar endophytic ...

    African Journals Online (AJOL)

    Endophytic fungi that reside in plant tissues are a potential source of secondary metabolites with biological activities. In our study, we investigated the detection of the antioxidant activity of the crude fungal extract of the genera Cladosporium, Alternaria, Aspergillus and Penicillium: endophytic fungi isolated from the leaves of ...

  17. Cytotoxic, anti-inflammatory and antioxidant activities of four different ...

    African Journals Online (AJOL)

    Purpose: To evaluate the cytotoxic, anti-inflammatory and antioxidant activities of four different solvent extracts obtained from the aerial parts of Galega officinalis L. Methods: The hexane, DCM, methanol and water extracts of G. officinalis were successively obtained by soxhlet extraction method. The cytotoxic activity of the ...

  18. Evaluation of the antioxidant and antityrosinase activities of three ...

    African Journals Online (AJOL)

    The antioxidant activities were performed on - carotene-linoleic acid, reducing power, 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferrous ions chelating abilities and xanthine oxidase inhibitory activity. In addition to this, phenolic compounds were also detected. The acetonic and methanolic extracts of P. nebrodensis showed the ...

  19. Evaluation of Antioxidant and Antifungal Activities of Polyphenol-rich ...

    African Journals Online (AJOL)

    Purpose: To evaluate the antioxidant and antifungal activities of polyphenol-rich extracts of the dried fruit pulp of Garcinia pedunculata (GP) and Garcinia morella (GM) to determine their traditional claims of therapeutic activity against certain diseases. Methods: Analysis of total phenolic (TP) and flavonoid (TF) contents of the ...

  20. Improvement of antioxidant and moisture-preserving activities of Sargassum horneri polysaccharide enzymatic hydrolyzates.

    Science.gov (United States)

    Shao, Ping; Chen, Xiaoxiao; Sun, Peilong

    2015-03-01

    In the previous study, we have found that polysaccharides isolated from Sargassum horneri exhibited bioactivities. The aim of this study was to investigate the antioxidant and moisture-preserving activities of molecular weight alteration of Sargassum horneri polysaccharide in vitro. For this purpose, the homogeneous active polysaccharide SHP was isolated from Sargassum horneri, and response surface methodology was employed to optimize the enzymatic degradation conditions to get SHP-derived fragments with different molecular weight. Results proved that the polysaccharide is capable of scavenging both ABTS and DPPH radicals in vitro. The study revealed that the polysaccharides had strong moisture-absorption and -retention capacities as compared to propanediol and glycerin. Furthermore, these data demonstrated that molecular weight had a certain effect on antioxidant activities and strong moisture-retention capacities of the polysaccharide from Sargassum horneri. Copyright © 2014 Elsevier B.V. All rights reserved.

  1. Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae

    Directory of Open Access Journals (Sweden)

    F. Xavier Malcata

    2013-04-01

    Full Text Available A growing market for novel antioxidants obtained from non-expensive sources justifies educated screening of microalgae for their potential antioxidant features. Characterization of the antioxidant profile of 18 species of cyanobacteria (prokaryotic microalgae and 23 species of (eukaryotic microalgae is accordingly reported in this paper. The total antioxidant capacity, accounted for by both water- and lipid-soluble antioxidants, was evaluated by the (radical cation ABTS method. For complementary characterization of cell extracts, a deoxyribose assay was carried out, as well as a bacteriophage P22/Salmonella-mediated approach. The microalga Scenedesmus obliquus strain M2-1 exhibited the highest (p > 0.05 total antioxidant capacity (149 ± 47 AAU of intracellular extracts. Its scavenger activity correlated well with its protective effects against DNA oxidative damage induced by copper(II-ascorbic acid; and against decay in bacteriophage infection capacity induced by H2O2. Finally, performance of an Ames test revealed no mutagenic effects of the said extract.

  2. The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilms.

    Science.gov (United States)

    Peralta, Mariana Andrea; Ortega, María Gabriela; Cabrera, José Luis; Paraje, María Gabriela

    2018-02-21

    The antioxidant effect of 8PP, a prenylflavonoid from Dalea elegans on Candida albicans biofilms, was investigated. We previously reported that sensitive (SCa) and resistant C. albicans (RCa) biofilms were strongly inhibited by this compound, in a dose-depending manner (50 μM-100 μM), with a prooxidant effect leading to accumulation of endogenous oxidative metabolites and increased antioxidant defenses. In this work, the antifungal activity of high concentrations of 8PP (200-1000 μM), the cellular stress imbalance and the architecture of biofilms were evaluated. Biofilms were studied by crystal violet and confocal scanning laser microscopy (CSLM) with COMSTAT analysis. Superoxide anion radical, the activity of the superoxide dismutase and the total antioxidant capacity were measured. Intracellular ROS were detected by a DCFH-DA and visualized by CSLM; reactive nitrogen intermediates by Griess. An antioxidant effect was detected at 1000 μM and levels of oxidant metabolites remained low, with major changes in the SCa. COMSTAT analysis showed that biofilms treated with higher concentrations exhibited different diffusion distances with altered topographic surface architectures, voids, channels and pores that could change the flow inside the matrix of biofilms. We demonstrate for first time, a concentration-dependent antioxidant action of 8PP, which can alter its antifungal activity on biofilms. Copyright © 2018 Elsevier Ltd. All rights reserved.

  3. Determining antioxidant activities of lactobacilli cell-free supernatants by cellular antioxidant assay: a comparison with traditional methods.

    Directory of Open Access Journals (Sweden)

    Jiali Xing

    Full Text Available Antioxidant activity of lactic acid bacteria is associated with multiple health-protective effects. Traditional indexes of chemical antioxidant activities poorly reflect the antioxidant effects of these bacteria in vivo. Cellular antioxidant activity (CAA assay was used in this study to determine the antioxidant activity of cell-free supernatants (CFSs of 10 Lactobacillus strains. The performance of the CAA assay was compared with that of four chemical antioxidant activity assays, namely, DPPH radical scavenging, hydroxyl radical scavenging (HRS, reducing power (RP, and inhibition of linoleic acid peroxidation (ILAP. Results of the CAA assay were associated with those of DPPH and ILAP assays, but not with those of RP and HRS assays. The inter- and intra-specific antioxidant activities of CFS were characterized by chemical and CAA assays. L. rhamnosus CCFM 1107 displayed a high antioxidative effect similar to positive control L. rhamnosus GG ATCC 53103 in all of the assays. The CAA assay is a potential method for the detection of antioxidant activities of lactobacilli CFSs.

  4. Evaluation of the antioxidant, anti-inflammatory, and anticancer activities of Euphorbia hirta ethanolic extract.

    Science.gov (United States)

    Sharma, Neelesh; Samarakoon, Kalpa W; Gyawali, Rajendra; Park, Yang-Ho; Lee, Sung-Jin; Oh, Sung Jong; Lee, Tae-Hoon; Jeong, Dong Kee

    2014-09-15

    This study evaluated the chemical composition, antioxidant, anti-inflammatory and anticancer activities of a Euphorbia hirta L. extract. The antioxidant activities of whole E. hirta ethanol extract were determined by electron spin resonance spectrophotometric analysis of 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl, and alkyl radical levels and by using an online high-performance liquid chromatography (HPLC)-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay. The E. hirta ethanol extract (0.5 mg/mL) exhibited DPPH-scavenging activity of 61.19% ± 0.22%, while the positive control (0.5 mg/mL ascorbic acid) had 100% ± 0.22% activity. The concentration of the extract required to trap 50% of DPPH (IC50) was 0.205 mg/mL. Online HPLC analysis of the extract also showed strong antioxidant activity. The anti-inflammatory activity of the E. hirta extract was assessed in lipopolysaccharide-induced RAW 264.7 macrophages. The anti-inflammatory activity was highest in the presence of 200 µg/mL E. hirta extract, and nitric oxide production was decreased significantly (p hirta may warrant further investigation for the development of antioxidant, anti-inflammatory, and anticancer herbal medications.

  5. Antioxidant Activity of Phenolic Compounds from Fava Bean Sprouts.

    Science.gov (United States)

    Okumura, Koharu; Hosoya, Takahiro; Kawarazaki, Kai; Izawa, Norihiko; Kumazawa, Shigenori

    2016-06-01

    Fava beans are eaten all over the world and recently, marketing for their sprouts began in Japan. Fava bean sprouts contain more polyphenols and l-3,4-dihydroxyphenylalanine (l-DOPA) than the bean itself. Our antioxidant screening program has shown that fava bean sprouts also possess a higher antioxidant activity than other commercially available sprouts and mature beans. However, the individual constituents of fava bean sprouts are not entirely known. In the present study, we investigated the phenolic compounds of fava bean sprouts and their antioxidant activity. Air-dried fava bean sprouts were treated with 80% methanol and the extract was partitioned in water with chloroform and ethyl acetate. HPLC analysis had shown that the ethyl acetate-soluble parts contained phenolic compounds, separated by preparative HPLC to yield 5 compounds (1-5). Structural analysis using NMR and MS revealed that the compounds isolated were kaempferol glycosides. All isolated compounds had an α-rhamnose at the C-7 position with different sugars attached at the C-3 position. Compounds 1-5 had β-galactose, β-glucose, α-rhamnose, 6-acetyl-β-galactose and 6-acetyl-β-glucose, respectively, at the C-3 position. The amount of l-DOPA in fava bean sprouts was determined by the quantitative (1) H NMR technique. The l-DOPA content was 550.45 mg ± 11.34 /100 g of the raw sprouts. The antioxidant activities of compounds 2-5 and l-DOPA were evaluated using the 2,2-diphenyl-1-picrylhydrazyl scavenging assay. l-DOPA showed high antioxidant activity, but the isolated kaempferol glycosides showed weak activity. Therefore, it can be suggested that l-DOPA contributed to the antioxidant activity of fava bean sprouts. © 2016 Institute of Food Technologists®

  6. Antioxidant activity and potential photoprotective from amazon native flora extracts.

    Science.gov (United States)

    Martins, Francislene J; Caneschi, César A; Vieira, José L F; Barbosa, Wagner; Raposo, Nádia R B

    2016-08-01

    Plant species are sources of active compounds that can fight and/or prevent damage caused by reactive oxygen species, which enables the development of natural products that can help to prevent premature aging caused by exposure to solar radiation. This study assessed the antioxidant and photoprotective activities of six dried extracts of plants from the Brazilian Amazon biome. Plant extracts were prepared in 70% (v/v) ethanol by dynamic maceration for 72h in the dark, and then filtered, concentrated and lyophilized. The extracts were subjected to a phytochemical screening. The antioxidant activity was measured using a 2,2-diphenyl-1-picrylhydrazyl assay and the photoprotection assay was performed using the diffuse transmittance technique. The data obtained from the antioxidant activity assay was evaluated by Student's t-test for independent samples, with the aid of Statistical Package for Social Sciences v.14.0 for Windows software. The flavonoids represent a special metabolites class present in all analyzed extracts. The antioxidant activity (μgmL(-1)) decreased in the following order: Aniba canelilla (1.80±0.16), Brosimum acutifolium (2.84±0.38), Dalbergia monetaria (5.46±0.17) or Caesalpinia pyramidalis (6.45±1.18), Arrabidaea chica (15.35±0.86), and Aspidosperma nitidum (99.14±2.3). Only D. monetaria showed a considerable sun protection factor allowing for labeling (6.0±0.3). The D. monetaria extract was considered the most promising sample because it had optimal antioxidant and photoprotective activities against solar radiation, considering the limit established by regulatory agencies. These extracts with antioxidant potential can be used in photoprotective formulations, providing synergistic photoprotective effect or elevating the adeed value of the product. Additionally, these formulations are attractive to a population who searchs for products made with natural ingredients. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. Evaluation of the Antioxidant Activity of the Marine Pyrroloiminoquinone Makaluvamines.

    Science.gov (United States)

    Alonso, Eva; Alvariño, Rebeca; Leirós, Marta; Tabudravu, Jioji N; Feussner, Klaus; Dam, Miriam A; Rateb, Mostafa E; Jaspars, Marcel; Botana, Luis M

    2016-10-27

    Makaluvamines are pyrroloiminoquinones isolated from Zyzzya sponges. Until now, they have been described as topoisomerase II inhibitors with cytotoxic effects in diverse tumor cell lines. In the present work, seven makaluvamines were tested in several antioxidant assays in primary cortical neurons and neuroblastoma cells. Among the alkaloids studied, makaluvamine J was the most active in all the assays. This compound was able to reduce the mitochondrial damage elicited by the well-known stressor H₂O₂. The antioxidant properties of makaluvamine J are related to an improvement of the endogenous antioxidant defenses of glutathione and catalase. SHSY5Y assays proved that this compound acts as a Nrf2 activator leading to an improvement of antioxidant defenses. A low concentration of 10 nM is able to reduce the reactive oxygen species release and maintain a correct mitochondrial function. Based on these results, non-substituted nitrogen in the pyrrole plus the presence of a p -hydroxystyryl without a double bond seems to be the most active structure with a complete antioxidant effect in neuronal cells.

  8. Synergistic antioxidant activity of green tea with some herbs

    Directory of Open Access Journals (Sweden)

    Dheeraj P Jain

    2011-01-01

    Full Text Available Cardiovascular diseases, cancer, arthritis, etc. are caused by free radicals that are byproducts of metabolic pathways. Selected plants namely Vitis vinifera, Phyllanthus emblica L., Punica granatum, Cinnamomum cassia, Ginkgo biloba L., and Camellia sinensis Linn. are reported to produce antioxidant property. This study is undertaken to support the hypothesis that formulation of a polyherbal combination of these plants shows a synergistic effect with green tea. The extracts of each drug were characterized by phytochemical studies and tests for phenolics and flavonoids. In vitro antioxidant activity for individual drug and its combination was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH, superoxide, and nitric oxide free radical scavenging methods. Our results suggest that a combination of all these herbs with green tea can synergistically enhance antioxidant activity and thus lower doses of each herb with green tea may be used. Antioxidant potential of polyherbal combination was also comparable to that of standard ascorbic acid. Studies showed that selected individual plants contained abundant quantity of phenolics and flavonoids and their polyherbal combination with green tea was found to produce best antioxidant activity among all individual extracts. This will help in avoiding undesirable side effects due to higher doses of single herb.

  9. Elimination of seaweed odour and its effect on antioxidant activity

    Science.gov (United States)

    Keyimu, Xiren Guli; Abdullah, Aminah

    2014-09-01

    The objectives of this study were to determine the most effective method to remove odour from Sargassum muticum seaweeds and studied their antioxidant properties. Ten grams of wet seaweeds (10 grams dried seaweeds soaked in 100 ml water for 2 hours) were soaked in 100 mL of 1%, 3% and 5% of gum Arabic, rice flour, lemon juice, respectively, and 1% of vinegar. There effect of each treatment on antioxidant level were determined by using the total phenolic content (TPC), free radical scavenging ability expressed as a DPPH value, and oxygen radical absorbance capacity (ORAC) and compared to control seaweeds sample (soaked in water only). For sensory attribute, seven trained panellists were asked to evaluate the fishy odour of 11 treated seaweed samples. The fishy odour characteristics and antioxidant activity of treated seaweeds were compared against the control sample (soaked seaweeds), and subjected to statistical analysis. Results showed that 3% and 5% lemon juice and 5% rice flour were able to eliminate the fishy odour of seaweed. However, the antioxidant activity was significantly higher (Plemon juice compared to other treatments. Therefore, 5% of lemon juice-treated seaweeds contained the least fishy odour and retained the highest antioxidant activity.

  10. Chemical Constituents and Antioxidant Activity of Teucrium barbeyanum Aschers

    Directory of Open Access Journals (Sweden)

    Mohamed Ali A. Alwahsh

    2015-01-01

    Full Text Available The different extracts of the aerial parts of Teucrium barbeyanum Aschers. were investigated for the chemical constituents and antioxidant activities. The chemical investigation of the plant led to the isolation of eleven known compounds through column chromatography in which nine were flavonoids and the other two were simple phenolic compounds. The compounds were characterized using NMR techniques ( 1H, 13C, DEPT-135 and 90, COSY, HMQC, HMBC and NOESY, UV spectroscopy and EI/ESI spectrometry. The isolated compounds were identified as 5-hydroxy-3,6,7,4′-tetramethoxyflavone (1, salvigenin (2, 5-hydroxy-6,7,3′,4′-tetramethoxyflavone (3, chrysosplenetin (4, cirsilineol (5, cirsimaritin (6, cirsiliol (7, apigenin (8 and luteolin (9, in addition to methyl caffeate (10 and 4-hydroxybenzoic acid (11. The antioxidant activit of the extracts was evaluated using 2,2-Diphenyl,1-picrylhydrazyl (DPPH, 2,2′-Azino-bis(3-ethylbenzothiazoline 6 sulfonic acid diammonium salt (ABTS and ferric reducing antioxidant power (FRAP methods. Ethyl acetate and butanol extracts showed comparable antioxidant activity to known antioxidants; trolox and ascorbic acid and the highest total phenolic and flavonoid contents. The active components were extracted efficiently in 70% aqueous methanol after defatting procedure. This is the first time the aforementioned compounds are isolated from this plant, and there has been no previous report on the biological studies on this species.

  11. Evaluation of the Antioxidant Activity of the Marine Pyrroloiminoquinone Makaluvamines

    Directory of Open Access Journals (Sweden)

    Eva Alonso

    2016-10-01

    Full Text Available Makaluvamines are pyrroloiminoquinones isolated from Zyzzya sponges. Until now, they have been described as topoisomerase II inhibitors with cytotoxic effects in diverse tumor cell lines. In the present work, seven makaluvamines were tested in several antioxidant assays in primary cortical neurons and neuroblastoma cells. Among the alkaloids studied, makaluvamine J was the most active in all the assays. This compound was able to reduce the mitochondrial damage elicited by the well-known stressor H2O2. The antioxidant properties of makaluvamine J are related to an improvement of the endogenous antioxidant defenses of glutathione and catalase. SHSY5Y assays proved that this compound acts as a Nrf2 activator leading to an improvement of antioxidant defenses. A low concentration of 10 nM is able to reduce the reactive oxygen species release and maintain a correct mitochondrial function. Based on these results, non-substituted nitrogen in the pyrrole plus the presence of a p-hydroxystyryl without a double bond seems to be the most active structure with a complete antioxidant effect in neuronal cells.

  12. Liquid Fermentation of Ganoderma applanatum and Antioxidant Activity of Exopolysaccharides.

    Science.gov (United States)

    Zhong-Hua, Liu; Xiao-Ge, Hou; Jin-Hui, Zhao; Le, H E

    2015-01-01

    The medium composition and fermentation conditions of Ganoderma applanatum(GA) strain were optimized by the liquid shake flask fermentation, and the antioxidant activity of exopolysaccharides was investigated. The results showed that the optimal conditions of the liquid fermentation of GA strain were as follows: Carbon source was corn powder, nitrogen source was soy powder, the initial pH was 6.0, the inoculum size was 8%, the fermentation temperature was 32(o)C, the fermentation time was 7 d. The exopolysaccharides of GA strain could scavenge hydroxyl radicals(HR) and superoxide anion radicals(SAR), and the concentration of exopolysaccharides was positively related to the antioxidant activity.

  13. ANTIOXIDANT, ANTI-INFLAMMATORY ACTIVITY OF GEORGIAN LEGUMINOUS CROPS CULTURES.

    Science.gov (United States)

    Chkhikvishvili, I; Mamniashvili, T; Gogia, N; Enukidze, M; Machavariani, M; Sanikidze, T

    2017-11-01

    The aim of the study was to identify the common in Georgia leguminous crops culture with pronounced antioxidant, anti-inflammatory activity. The primary evaluation of the antiinflammatory effects of beans was performed on the experimental models of MDCK and Jurkat cells model systems. Extracts of various varieties of legumes (Beans "Kidney", Meadow beans, Beans Shulavera, Batumian beans, Beans "Udelebi", green peas, peas Shulavera, lentils Lens Culinaris, Soy) were added to the intact or incubated under oxidative stress conditions Jurkat and MDCK cells. Cells' vitality was determined by MTT test. On the basis of analysis of the obtained results, we concluded that: - Meadow beans extract (low doses) revealed cytoprotective effect on the intact and incubated under oxidative stress conditions immune (Jurkat) and epithelial (MDCK) cells. High antioxidant, cytoprotective activity of this extract correlates with high polyphenols content in it. - The extract of Shulavera beans did affect the intact Jurkat and MDCK cells, but showed pronounced cytoprotective activity on these cells incubated under the oxidative stress conditions. High antioxidant, cytoprotective activity of this extract correlates with high content of polyphenols in it. - Low dose of lentils Lens Culinaris extracts revealed cytoprotective activity on the incubated under oxidative stress conditions MDCK cells, but was inactive in case of intact MDCK and incubated in different conditions immune Jurkat cells. The selective antioxidant activity of this extract is related with its other constituent components, but not polyphenols. - Despite high polyphenols content and high antioxidant activity in vivo, Batumian beans revealed moderate cytoprotective activity on intact and incubated under oxidative stress conditions Jurkat cells, suppressive activity on the intact MDCK cells and was inactive in relation to the incubated under oxidative stress conditions MDCK cells. Based on these findings, we can identify

  14. Antioxidant and cytotoxic activities of three species of tropical seaweeds.

    Science.gov (United States)

    Chia, Yin Yin; Kanthimathi, M S; Khoo, Kong Soo; Rajarajeswaran, Jayakumar; Cheng, Hwee Ming; Yap, Wai Sum

    2015-09-29

    Three species of seaweeds (Padina tetrastromatica, Caulerpa racemosa and Turbinaria ornata) are widely consumed by Asians as nutraceutical food due to their antioxidant properties. Studies have shown that these seaweeds exhibit bioactivities which include antimicrobial, antiviral, anti-hypertensive and anticoagulant activities. However, investigations into the mechanisms of action pertaining to the cytotoxic activity of the seaweeds are limited. The aim of this study was to determine the antioxidant and cytotoxic activities of whole extracts of P. tetrastromatica, C. racemosa and T. ornata, including the cellular events leading to the apoptotic cell death of the extract treated-MCF-7 cells. Bioassay guided fractionation was carried out and the compounds identified. Powdered samples were sequentially extracted for 24 h. Their antioxidant activities were assessed by the DPPH radical, superoxide, nitric oxide and hydroxyl radical scavenging assays. The cytotoxic activity of the extract-treated MCF-7cells was assessed using the MTT assay. The most potent fraction was subjected to bioassay guided fractionation with column chromatography. All the fractions were tested for cytotoxic activity, caspase activity and effect on DNA fragmentation. All three seaweeds showed potent radical scavenging activities in the various assays. The activity of the cellular antioxidant enzymes, superoxide dismutase, catalase and glutathione reductase, in MCF-7 cells, decreased in a time-dependent manner. The partially purified fractions exhibited higher cytotoxic activity, as assessed by the MTT assay, than the whole extracts in the breast adenocarcinoma cell line, MCF-7. LC-MS analysis revealed the presence of bioactive alkaloids such as camptothecin, lycodine and pesudopelletierine. Based on the results obtained, all three seaweeds are rich sources of enzymatic and non-enzymatic antioxidants which could contribute to their reported medicinal benefits.

  15. Antioxidant potential of water hyacinth (Eichornia crassipes): In vitro antioxidant activity and phenolic composition

    DEFF Research Database (Denmark)

    Surendraraj, A.; Farvin, Sabeena; Anandan, R.

    2013-01-01

    The aims of the present study were (a) to extract and quantify the main phenolic acids and tocopherols from the petiole, leaves, and flowers of Eichornia crassipes; (b) to evaluate the antioxidant capacity of the extracts in four in vitro systems (1,1-diphenyl-2-pycryl-hydrazyl [DPPH] radical...... and in the antioxidant activities of extracts from the various parts of E. crassipes. Out of the 11 phenolic acids analyzed, ethanolic extracts contained high amounts of gallic, protocatechuic, gentisic, and p-hydroxybenzoic acid, whereas, water extracts contained less amounts of a varied number of phenolic acids....... Ethanolic extracts of flower, which contained the highest total phenolic content, were found to have high DPPH radical scavenging activity and reducing power. However, ethanolic extracts of leaf exerted a high Fe2+ chelating activity and also inhibited lipid peroxidation process both in liposomes and fish...

  16. Hop pellets as an interesting source of antioxidant active compounds

    Directory of Open Access Journals (Sweden)

    Andrea Holubková

    2013-02-01

    Full Text Available Hop is a plant used by humankind for thousands of years. This plant is one of the main and indispensable raw materials for the beer production. It is used for various dishes preparation in the cuisine. Hop is also used to inhibit bacterial contamination. The hop extracts are used for its sedative, antiseptic and antioxidant properties in medicine, as a part of many phytopharmaceuticals. The present paper have focused on the extraction of polyphenolic compounds from 4 samples of hop pellets varieties of Aurora, Saaz, Lublin and Saphir, on the analyzing of bioactive substances (polyphenolics and flavonoids in prepared extracts and on the determination of antioxidant activity.  The highest content of polyphenolic substances was determined in the sample Lublin (153.06 mg gallic acid (GAE/g and Saaz (151.87 mg GAE/g. The amount of flavonoids in the samples  was descending order Saaz > Saphir > Aurora > Lublin. Hops, as plant, is known by high content of antioxidant active substances. Antioxidant activity was determined using three independent spectrofotometric methods, radical scavenging assays using 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS and 1,1-diphenyl-2-picrylhydrazyl (DPPH radical and ferric reducing antioxidant power (FRAP. The sample Aurora showed the highest ability to scavenge of ABTS radical cation. Antioxidant activity continued to decline in a row Saphir> Lublin> Saaz. The same trend was also observed by using the FRAP assay. The most effective DPPH radical scavengering activity had the sample Saaz a Saphir (p>0.05.doi:10.5219/270 Normal 0 21 false false false SK X-NONE X-NONE

  17. Compounds from Sedum caeruleum with antioxidant, anticholinesterase, and antibacterial activities.

    Science.gov (United States)

    Bensouici, Chawki; Kabouche, Ahmed; Karioti, Anastasia; Öztürk, Mehmet; Duru, Mehmet Emin; Bilia, Anna Rita; Kabouche, Zahia

    2016-01-01

    This is the first study on the phytochemistry, antioxidant, anticholinesterase, and antibacterial activities of Sedum caeruleum L. (Crassulaceae). The objective of this study is to isolate the secondary metabolites and determine the antioxidant, anticholinesterase, and antibacterial activities of S. caeruleum. Six compounds (1-6) were isolated from the extracts of S. caeruleum and elucidated using UV, 1D-, 2D-NMR, and MS techniques. Antioxidant activity was investigated using DPPH(•), CUPRAC, and ferrous-ions chelating assays. Anticholinesterase activity was determined against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes using the Ellman method. Antibacterial activity was performed according to disc diffusion and minimum inhibitory concentration (MIC) methods. Isolated compounds were elucidated as ursolic acid (1), daucosterol (2), β-sitosterol-3-O-β-D-galactopyranoside (3), apigenin (4), apigetrin (5), and apiin (6). The butanol extract exhibited highest antioxidant activity in all tests (IC50 value: 28.35 ± 1.22 µg/mL in DPPH assay, IC50 value: 40.83 ± 2.24 µg/L in metal chelating activity, and IC50 value: 23.52 ± 0.44 µg/L in CUPRAC), and the highest BChE inhibitory activity (IC50 value: 36.89 ± 0.15 µg/L). Moreover, the chloroform extract mildly inhibited (MIC value: 80 µg/mL) the growth of all the tested bacterial strains. Ursolic acid (1), daucosterol (2), β-sitosterol-3-O-β-D-galactopyranoside (3), apigenin (4), apigetrin (5), and apiin (6) were isolated from Sedum caeruleum for the first time. In addition, a correlation was observed between antioxidant and anticholinesterase activities of bioactive ingredients of this plant.

  18. Essential oil composition and antioxidant activity of Pterocarya fraxinifolia.

    Science.gov (United States)

    Ebrahimzadeh, M A; Nabavi, S F; Nabavi, S M

    2009-07-01

    Current research into free radicals has confirmed that plants rich in antioxidants play an essential role in the prevention of many diseases. The potential antioxidant activities of Pterocarya fraxinifolia bark and leaves investigated employing six in vitro assay systems. IC50 for DPPH radical-scavenging activities were 3.89 +/- 0.09 for leaves and 41.57 +/- 1.30 microg mL(-1) for bark, respectively. The leaf extract exhibited a good reducing power at 2.5 and 80 microg mL(-1) that was comparable with Vit C (p > 0.05). The extracts also showed weak nitric oxide-scavenging activity and Fe2+ chelating ability. The peroxidation inhibition of extracts exhibited values from 92 to 93% at 72nd h, almost at the same pattern of Vitamin C activity (p > 0.05). Based on higher total phenol and flavonoid contents in leaves, higher antioxidant activities were observed in leaf extract. In addition, chemical composition of leaf essential oil was determined. The major compound was bisabolol oxide A (23.6%). Sesquiterpenes and monoterpenes are the major compounds in leaves essential oil. Presence of these compounds may be a reason for the good antioxidant activity of leaf extract.

  19. Seasonal Difference in Antioxidant Capacity and Active Compounds Contents of Eucommia ulmoides Oliver Leaf

    Directory of Open Access Journals (Sweden)

    Jingfang Zhang

    2013-02-01

    Full Text Available Leaf of Eucommia ulmoides Oliver (EU is a Traditional Chinese Medicine and a functional food in China. Antioxidant contents of EU leaves, which were collected monthly during the period of May–October in three years, were determined. Samples’ antioxidant capacity was characterized by DPPH radical scavenging activity, hydroxyl radical scavenging activity, ferrous chelating ability, and antioxidant capacity in linoleic acid emulsion and in rapeseed oil assays. The results showed that contents of some active compounds and antioxidant activity were related to a certain time of the year. Samples collected in August showed high content of phenolics, and the samples collected in May contained higher amount of flavonoids than other samples. Leaves collected in May or June exhibited high contents of rutin, quercetin, geniposidic acid and aucubin. The August leaves showed stable and high DPPH radical scavenging activity, and ferrous chelating ability. May samples showed strong inhibitory effects on oxidation of rapeseed oil and linoleic acid. The DPPH radical scavenging activity was related to the total phenolics content. Flavonoids played an important role in the inhibitory effects on rapeseed oil and linoleic acid oxidation. Therefore, August and May were indicated as the best months to harvest EU leaves for industry.

  20. Yucca schidigera bark: phenolic constituents and antioxidant activity.

    Science.gov (United States)

    Piacente, Sonia; Montoro, Paola; Oleszek, Wieslaw; Pizza, Cosimo

    2004-05-01

    Two new phenolic constituents with unusual spirostructures, named yuccaols D (1) and E (2), were isolated from the MeOH extract of Yucca schidigera bark. Their structures were established by spectroscopic (ESIMS and NMR) analysis. The new yuccaols D and E, along with resveratrol (3), trans-3,3',5,5'-tetrahydroxy-4'-methoxystilbene (4), yuccaols A-C (5-7), yuccaone A (8), larixinol (9), the MeOH extract of Yucca schidigera bark, and the phenolic portion of this extract, were assayed for antioxidant activity by measuring the free radical scavenging effects using two different assays, namely, the Trolox Equivalent Antioxidant Capacity (TEAC) assay and the coupled oxidation of beta-carotene and linoleic acid (autoxidation assay). The significant activities exhibited by the phenolic fraction and its constituents in both tests show the potential use of Y. schidigera as a source of antioxidant principles.

  1. Chilean prosopis mesocarp flour: phenolic profiling and antioxidant activity.

    Science.gov (United States)

    Schmeda-Hirschmann, Guillermo; Quispe, Cristina; Soriano, Maria Del Pilar C; Theoduloz, Cristina; Jiménez-Aspée, Felipe; Pérez, Maria Jorgelina; Cuello, Ana Soledad; Isla, Maria Inés

    2015-04-17

    In South America, the mesocarp flour of Prosopis species plays a prominent role as a food resource in arid areas. The aim of this work was the characterization of the phenolic antioxidants occurring in the pod mesocarp flour of Chilean Prosopis. Samples were collected in the Copiapo, Huasco and Elqui valleys from the north of Chile. The samples of P. chilensis flour exhibited a total phenolic content ranging between 0.82-2.57 g gallic acid equivalents/100 g fresh flour weight. The highest antioxidant activity, measured by the DPPH assay, was observed for samples from the Huasco valley. HPLC-MS/MS analysis allowed the tentative identification of eight anthocyanins and 13 phenolic compounds including flavonol glycosides, C-glycosyl flavones and ellagic acid derivatives. The antioxidant activity and the phenolic composition in the flour suggest that this ancient South American resource may have potential as a functional food.

  2. Chilean Prosopis Mesocarp Flour: Phenolic Profiling and Antioxidant Activity

    Directory of Open Access Journals (Sweden)

    Guillermo Schmeda-Hirschmann

    2015-04-01

    Full Text Available In South America, the mesocarp flour of Prosopis species plays a prominent role as a food resource in arid areas. The aim of this work was the characterization of the phenolic antioxidants occurring in the pod mesocarp flour of Chilean Prosopis. Samples were collected in the Copiapo, Huasco and Elqui valleys from the north of Chile. The samples of P. chilensis flour exhibited a total phenolic content ranging between 0.82–2.57 g gallic acid equivalents/100 g fresh flour weight. The highest antioxidant activity, measured by the DPPH assay, was observed for samples from the Huasco valley. HPLC-MS/MS analysis allowed the tentative identification of eight anthocyanins and 13 phenolic compounds including flavonol glycosides, C-glycosyl flavones and ellagic acid derivatives. The antioxidant activity and the phenolic composition in the flour suggest that this ancient South American resource may have potential as a functional food.

  3. Comparison of Antioxidant Activities of Melanin Fractions from Chestnut Shell

    Directory of Open Access Journals (Sweden)

    Zeng-Yu Yao

    2016-04-01

    Full Text Available Chestnut shell melanin can be used as a colorant and antioxidant, and fractionated into three fractions (Fr. 1, Fr. 2, and Fr. 3 with different physicochemical properties. Antioxidant activities of the fractions were comparatively evaluated for the first time. The fractions exhibited different antioxidative potential in different evaluation systems. Fr. 1, which is only soluble in alkaline water, had the strongest peroxidation inhibition and superoxide anion scavenging activity; Fr. 2, which is soluble in alkaline water and hydrophilic organic solvents but insoluble in neutral and acidic water, had the greatest power to chelate ferrous ions; and Fr. 3, which is soluble both in hydrophilic organic solvents and in water at any pH conditions, had the greatest hydroxyl (·OH and 1,1-diphenyl-2-picryl-hydrazyl (DPPH· radicals scavenging abilities, reducing power, and phenolic content. The pigment fractions were superior to butylated hydroxytolune (BHT in ·OH and DPPH· scavenging and to ethylene diamine tetraacetic acid (EDTA in the Fe2+–chelation. They were inferior to BHT in peroxidation inhibition and O2·− scavenging and reducing power. However, BHT is a synthetic antioxidant and cannot play the colorant role. The melanin fractions might be used as effective biological antioxidant colorants.

  4. Antioxidant and anticholinesterase activities of eleven edible plants.

    Science.gov (United States)

    Boğa, Mehmet; Hacıbekiroğlu, Işıl; Kolak, Ufuk

    2011-03-01

    Consumers have become more interested in beneficial effects of vegetables, fruits, and tea to protect their health. The antioxidant potential and anticholinesterase activity of eleven edible plants were investigated. The dichloromethane, ethanol and water extracts prepared from celery [Apium graveolens L. (Umbelliferae)], Jerusalem artichoke [Helianthus tuberosus L. (Compositae)], spinach [Spinacia oleracea L. (Chenopodiaceae)], chard [Beta vulgaris L. var. cicla (Chenopodiaceae)], purslane [Portulaca oleracea L. (Portulacaceae)], ispit, or borage [Trachystemon orientale (L.) G. Don (Boraginaceae)], garden rocket [Eruca sativa Mill. (Brassicaceae)], red cabbage [Brassica oleracea L. var. capitata f. rubra DC. (Cruciferae)], lime flower [Tilia tomentosa Moench (Tiliaceae)], cinnamon [Cinnamomum cassia Presl. (Lauraceae)], and rosehip [Rosa canina L. (Rosaceae)], were tested to determine their antioxidant and anticholinesterase activities by using CUPRAC (cupric reducing antioxidant capacity) and Ellman methods, respectively, for the first time. As a result, the dichloromethane, ethanol and water extracts of cinnamon showed the best antioxidant effect among the extracts of the tested plants. The ethanol extract of cinnamon exhibited 63.02% inhibition against acetylcholinesterase and 85.11% inhibition against butyrylcholinesterase (BChE) at 200 µg/mL concentration while the dichloromethane extract of garden rocket possessed the highest inhibition (91.27%) against BChE among all the tested extracts. This study indicated that the ethanol extract of cinnamon may be a new potential resource of natural antioxidant and anticholinesterase compounds.

  5. Isolation, purification and antioxidant activity of polysaccharides from the leaves of maca (Lepidium Meyenii).

    Science.gov (United States)

    Caicai, Kang; Limin, Hao; Liming, Zhang; Zhiqiang, Zheng; Yongwu, Yang

    2018-02-01

    Two fractions of polysaccharides (MLP-1 and MLP-2) were extracted from the leaves of maca (Lepidium Meyenii Walp.) by water, and purified using DEAE-52 ion exchange resin and sephadex G-200 clumns chromatography. An investigation was carried out for their structural characterization and antioxidant activity in vitro. The results indicated that MLP-1 was mainly composed of ribose, rhamnose, arabinose, xylose, mannose, glucose and galactose, with the molar ratio of 0.12:0.32:1.50:0.32:1.03:1.00:0.93; the MLP-2 was a homopolysaccharide composed of glucose. The molecular weight (Mw) of MLP-1 was 42756Da, and the Mw of MLP-2 was 93541Da. The FT-IR spectra showed the general characteristic absorption peak of maca leaf polysaccharides (MLPs). The evaluation of antioxidant activity revealed that MLP-1 had strong scavenging effects in vitro on hydroxyl, superoxide anion and DPPH radicals, whose EC 50 (mg/mL) was 0.44, 0.21, and 0.82, respectively. Both MLP-1 and MLP-2 presented dose-dependently positive effects on the antioxidant-related parameters. The results suggested that the purified MLP-1 displayed better antioxidant capacities than that of MLP-1, which could be explored as potential antioxidant agents for the complementary medicine or functional foods. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Study of Convolvulus pluricaulis for antioxidant and anticonvulsant activity.

    Science.gov (United States)

    Verma, Sristi; Sinha, Reema; Kumar, Puspendra; Amin, Faizal; Jain, Jainendra; Tanwar, Shivani

    2012-03-01

    Convolvulus pluricaulis Choisy is a perennial wild herb commonly found on sandy & rocky areas under xerophytic conditions in northern India. It is a reputed drug of ayurveda and reported to posses antioxidant, brain tonic, nervine tonic, laxative and has been used in anxiety, neurosis, epilepsy, insomnia, burning sensation, oedema and urinary disorders. In the present study, methanolic extract of whole plant of Convolvulus pluricaulis Choisy was evaluated for antioxidant activity by using 1, 1-diphenyl-2-picryl- hydrazyl (DPPH) free radical scavenging model and anticonvulsant activity by using maximal electroshock seizure model. In antioxidant activity, ascorbic acid was used as standard agent while results of anticonvulsant studies were compared with phenytoin. Results of antioxidant activity have demonstrated significant free radical scavenging effect for methanolic extract of Convolvulus pluricaulis Choisy. IC50 value of methanolic extract was observed as 41.00μg/ml as compared to 2.03μg/ml of ascorbic acid. Methanolic extract of C. pluricaulis was evaluated for anticonvulsant activity at 250, 500 and 1000mg/kg. Experimental results have shown that at the dose of 500 and 1000mg/kg, C. pluricaulis didn't abolish the hind limb extension, but reduced the mean recovery time from convulsion.

  7. Anibacterial, antibiofilm and antioxidant activity of Potamogeton nodosus Poir. extracts

    Directory of Open Access Journals (Sweden)

    Đurđević Jelena

    2014-01-01

    Full Text Available Antibacterial, antibiofilm and antioxidant activity of acetone, ethanol, ethyl acetate and diethyl ether extracts of P. nodosus Poir. were tested. In vitro antibacterial activity was investigated by microdilution method. Minimum inhibitory concentration (MIC and minimum bactericidal concentration (MBC have been determined. The values were in the range from 5 to > 5 mg/ml. Antibiofilm activity was tested by crystal violet assay. The biofilm inhibitory concentration (BIC values were from 2.03 to > 10 mg/ml. The best results against biofilm formation had ethanol and ethyl acetate extracts. The total phenolic content was established by using Folin-Ciocalteu method. The highest total phenol content was found in the ethyl acetate extract (28.45 mg GA/g while the lowest content in the ethanol extract (22.74 mg GA/g. The concentrations of flavonoids were determined using aluminium chloride method. The concentration of total flavonoid content was 102.09 mg RU/g for the ethyl acetate extract, while the lowest content was noticed in diethyl ether extract (32.93 mg RU/g. The antioxidant activity was determined by the DPPH free radicals assay. Antioxidative efficiency was in the range from 641.77 to 8811.63 μg/ml. The tested extracts showed weak antioxidant activity (AAI < 0.5.

  8. Anticancer and antioxidant activities of methanol extracts and fractions of some Cameroonian medicinal plants.

    Science.gov (United States)

    Tagne, Richard Simo; Telefo, Bruno Phelix; Nyemb, Jean Noel; Yemele, Didiane Mefokou; Njina, Sylvain Nguedia; Goka, Stéphanie Marie Chekem; Lienou, Landry Lienou; Nwabo Kamdje, Armel Hervé; Moundipa, Paul Fewou; Farooq, Ahsana Dar

    2014-09-01

    To obtain a scientific basis of the use of plant-derived preparations by many rural people in Cameroon, for their primary health care needs in the treatment of diseases such as cancer. The antiproliferative effect of 11 plants methanol crude extracts on four cancer cells using sulforhodamine-B assay and their antioxidant activities using 1-diphenyl-2-picrylhydrazyl radical and nitric oxide radical scavenging ability were investigated. The Ekebergia senegalensis (E. senegalensis) and Protea elliotii (P. elliotii) extracts were selected based on their antioxidant and anticancer activities, and partition in hexane, dichloromethane, ethyl acetate, butanol and methanol was done. Each fraction was submitted to antioxidant and anticancer activities, and the effect of the dichloromethane fraction (the most antiproliferative fraction) on NCI-H460 cell cycle was determined by flow cytometry. The most antiproliferative substances were found for the extracts from E. senegalensis, P. elliotii, Terminalia macroptera and Vitellaria paradoxa. Whereas the most antioxidant substances were found for the extracts from Cissus populnea, E. senegalensis, P. elliotii, Terminalia macroptera, Vitellaria paradoxa, and Gardenia aqualla. Dichloromethane fraction of P. elliotii was found to be highly antiproliferative to NCI-H460 cancer cells and showed S phase arrest cell cycle progression. Ethyl acetate n-butanol and methanol fractions showed quite strong antioxidant activity for both E. senegalensis and P. elliotii, as compared to that of gallic acid. Overall, the antiproliferative and antioxidant activities of some of the extracts lend some support to their use in the traditional medicine of Adamawa Region, Cameroon to treat cancer. Copyright © 2014 Hainan Medical College. Published by Elsevier B.V. All rights reserved.

  9. Antioxidant potential of water hyacinth (Eichornia crassipes): In vitro antioxidant activity and phenolic composition

    DEFF Research Database (Denmark)

    Surendraraj, Alagarsamy; Farvin, Sabeena; Anandan, R.

    2011-01-01

    The aims of the present study were (a) to extract and quantify the main phenolic acids and tocopherols from the petiole, leaf and flowers of Eichornia crassipes, (b) to evaluate the antioxidant capacity of the extracts in four in vitro systems (DPPH radical scavenging ability, iron chelating...... activity, reducing power and prevention of oxidation in a liposome model system) and its effectiveness in retarding lipid peroxidation in fish oil by accelerated stability test. Significant differences were observed in total and individual phenolic content and antioxidant activities of extracts from...... the various parts of E. crassipes. Out of the 11 phenolic acids analysed, ethanolic extracts contained high amounts gallic, protocatechuic, gentisic and phydroxybenzoic acid, whereas, water extracts contained less amounts of varied number of phenolic acids. Ethanolic extracts of flower, which contained...

  10. Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae

    Directory of Open Access Journals (Sweden)

    G. R. Souza

    2017-08-01

    Full Text Available Abstract This study was carried out to assess the antibacterial, cytotoxic and antioxidant activities of extracts of Morus nigra L. HPLC was used to determine the fingerprint chromatogram of the crude ethanolic extract (Mn-EtOH. The antibacterial effect was assessed through the method of microdilution. The cytotoxicity was tested against human tumour cell lines using the 3-(4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2H-tetrazolium bromide (MTT assay. The total phenolic and flavonoid contents were also assessed through the Folin-Ciocalteu and aluminum chloride methods, respectively. Antioxidant activities of the extracts were evaluated by using 2,2-diphenyl-1-picrylhydrazil (DPPH radical scavenging and β-carotene-linoleic acid bleaching methods. The presence of phenolic compounds in Mn-EtOH was confirmed using HPLC. The extracts showed activity against most microorganisms tested. The extracts did not show any expressive antiproliferative effect in the assessment of cytotoxicity. The most significant total phenolic content was 153.00 ± 11.34 mg of gallic acid equivalent/g to the ethyl acetate extract (AcOEt. The total flavonoid content was 292.50 ± 70.34 mg of catechin equivalent/g to the AcOEt extract, which presented the best antioxidant activity (IC50 50.40 ± 1.16 μg/mL for DPPH scavenging. We can conclude that this species shows strong antibacterial and antioxidant activities, as well as weak cytotoxic effects.

  11. Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae).

    Science.gov (United States)

    Souza, G R; Oliveira-Junior, R G; Diniz, T C; Branco, A; Lima-Saraiva, S R G; Guimarães, A L; Oliveira, A P; Pacheco, A G M; Silva, M G; Moraes-Filho, M O; Costa, M P; Pessoa, C Ó; Almeida, J R G S

    2017-08-17

    This study was carried out to assess the antibacterial, cytotoxic and antioxidant activities of extracts of Morus nigra L. HPLC was used to determine the fingerprint chromatogram of the crude ethanolic extract (Mn-EtOH). The antibacterial effect was assessed through the method of microdilution. The cytotoxicity was tested against human tumour cell lines using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The total phenolic and flavonoid contents were also assessed through the Folin-Ciocalteu and aluminum chloride methods, respectively. Antioxidant activities of the extracts were evaluated by using 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging and β-carotene-linoleic acid bleaching methods. The presence of phenolic compounds in Mn-EtOH was confirmed using HPLC. The extracts showed activity against most microorganisms tested. The extracts did not show any expressive antiproliferative effect in the assessment of cytotoxicity. The most significant total phenolic content was 153.00 ± 11.34 mg of gallic acid equivalent/g to the ethyl acetate extract (AcOEt). The total flavonoid content was 292.50 ± 70.34 mg of catechin equivalent/g to the AcOEt extract, which presented the best antioxidant activity (IC50 50.40 ± 1.16 μg/mL) for DPPH scavenging. We can conclude that this species shows strong antibacterial and antioxidant activities, as well as weak cytotoxic effects.

  12. Antioxidant activities of extracts from Barkleyanthus salicifolius (Asteraceae) and Penstemon gentianoides (Scrophulariaceae).

    Science.gov (United States)

    Domínguez, Mariana; Nieto, Antonio; Marin, Juan C; Keck, Anna-Sigrid; Jeffery, Elizabeth; Céspedes, Carlos L

    2005-07-27

    Various extracts of the aerial parts of Barkleyanthus salicifolius (Asteraceae) and Penstemon gentianoides (Scrophulariaceae) have been used in folk medicine to treat many ailments, particularly inflammation and migraine. Neither the bioactive components responsible nor the mechanisms involved have been evaluated. Here are reported antioxidant activities of their methanol, dichloromethane, and ethyl acetate extracts. Samples were evaluated for oxygen radical absorption capacity (ORAC), ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging, and inhibition of the formation of thiobarbituric acid reactive species (TBARS), a measure of lipid peroxidation. Antioxidant activities were strongly correlated with total polyphenol content. The most active extracts from P. gentianoides in scavenging DPPH radicals and inhibiting TBARS formation were the methanol extract (A) and a further ethyl acetate extract of this (E). Partition E was further divided into eight fractions, and both E and the fractions were compared for activity against butylated hydroxytoluene, quercetin, and tocopherol. Partition E and the most active fractions, 5 and 6, were found to have I(50) values of 14.1, 38.6, and 41.8 ppm, respectively, against DPPH and 18.5, 26.0, and 12.7 ppm, respectively, against TBARS formation. Consistent with this finding, partition E and fractions 4-6 had the greatest ORAC and FRAP values. These results show that these plants could be useful antioxidant sources.

  13. Phenolic Profiles and Antioxidant Activity of Lotus Root Varieties

    Directory of Open Access Journals (Sweden)

    Yang Yi

    2016-06-01

    Full Text Available Lotus root attracts increasing attention mainly because of its phenolic compounds known as natural antioxidants. Its thirteen varieties were systematically analyzed on the content, distribution, composition and antioxidant activity of phenolic compounds for a better understanding of this aquatic vegetable. The respective mean contents of total phenolics in their flesh, peel and nodes were 1.81, 4.30 and 7.35 mg gallic acid equivalents (GAE/g fresh weight (FW, and those of total flavonoids were 3.35, 7.69 and 15.58 mg rutin equivalents/g FW. The phenolic composition determined by a high-performance liquid chromatography method varied significantly among varieties and parts. The phenolics of flesh were mainly composed of gallocatechin and catechin; those of peel and node were mainly composed of gallocatechin, gallic acid, catechin and epicatechin. The antioxidant activities of phenolic extracts in increasing order were flesh, peel and node; their mean concentrations for 50% inhibition of 2,2-diphenyl-1-picrylhydrazyl radical were 46.00, 26.43 and 21.72 µg GAE/mL, and their mean values representing ferric reducing antioxidant power were 75.91, 87.66 and 100.43 µg Trolox equivalents/100 µg GAE, respectively. “Zoumayang”, “Baheou”, “No. 5 elian” and “Guixi Fuou” were the hierarchically clustered varieties with relatively higher phenolic content and stronger antioxidant activity as compared with the others. Especially, their nodes and peels are promising sources of antioxidants for human nutrition.

  14. Antimicrobial and antioxidant activity of lemon balm Kombucha

    OpenAIRE

    Velićanski Aleksandra S.; Cvetković Dragoljub D.; Markov Siniša L.; Tumbas Vesna T.; Savatović Slađana M.

    2007-01-01

    Kombucha is a beverage traditionally produced by metabolic activity of yeasts and acetic acid bacteria. The antimicrobial activity of lemon balm kombucha as well as of particular control samples was determined by agar-well diffusion method. Antioxidant activity on stable 1,1-diphenyl-2-picrylhydrazyl radicals of lemon balm kombucha and lemon balm tea was determined by electron spin resonance spectroscopy. Acetic acid, Kombucha samples and heat-denaturated kombucha showed significant antimicro...

  15. Essential Oil Composition, Antibacterial and Antioxidant Activities of ...

    African Journals Online (AJOL)

    picrylhydrazyl (DPPH) and deoxyribose degradation assays and was found to possess good radical scavenging activity with IC50 values of 11.2 and 3.74 μl/ml, respectively. Keywords: antibacterial, antioxidant, Mentha aquatica, essential oil, capillary gas ...

  16. Observation of antioxidant activity of leaves, callus and suspension ...

    African Journals Online (AJOL)

    GREGORY

    2011-12-16

    Dec 16, 2011 ... Antioxidant activity and phenolic compound was found in Justicia gendarussa via total phenolic content (TPC) and α,α-diphenyl-β-pycrilhydrazil hydrate (DPPH) radical scavenging assays. The assays were applied on aqueous and methanolic extracts of leaves, callus culture and cell suspension culture.

  17. Antibacterial and Antioxidant Activity of Fahrenheitia zeylanica (Thw ...

    African Journals Online (AJOL)

    Fahrenheitia zeylanica (Thw.) Airy is a lofty evergreen tree belonging to the family Euphorbiaceae. The present study was conducted to evaluate antibacterial and antioxidant activity of solvent extracts of leaves of F. zeylanica. The powdered leaf material was sequentially extracted using ethyl acetate, chloroform and ...

  18. Nanoemulsion of ethanolic extracts of propolis and its antioxidant activity

    Science.gov (United States)

    Mauludin, R.; Primaviri, D. S.; Fidrianny, I.

    2015-09-01

    Propolis contains several antioxidant compounds which can be used in topical application to protect skin against free radical and prevent skin cancer and skin aging. Ethanolic extracts of propolis (EEP) provided the greatest antioxidant activity but has very small solubility in water thus was prepared in nanoemulsion (NE). EEP contains steroid/triterpenoid, flavonoid, and saponin. EEP had the value of DPPH scavenging activity 61.14% and IC50 0.41629 ppm. The best NE formulation consisted of 26.25% Kolliphor RH40; 8.75% glycerin; 5% rice bran oil; and 3% EEP. NE was transparent, had particle size of 23.72 nm and polydispersity index of 0.338. Based on TEM morphology, NE was almost spherical and has particle size below 50 nm. NE propolis revealed to be physically stable after stability test within 63 days at 25°C and passed 6 cycles of Freeze and Thaw test without separated. NE propolis reduced around 58% of free radical DPPH similar to antioxidant activity of the original extracts. Antioxidant activity of NE propolis is relatively stable after stored for 6 weeks. NE propolis was proven to be safe by primary irritation test with the value of primary irritation index (OECD) was 0.

  19. Extraction and antioxidant activity of phenolic compounds from ...

    African Journals Online (AJOL)

    Purpose: To use response surface methodology to optimize the extraction of the phenolic compounds in wheat bran treated by steam explosion, and to determine the antioxidant activity of the extract obtained. Methods: By using response surface methodology, the effects of extraction time, methanol concentration ...

  20. Ultrasonic-Assisted Extraction and Antioxidant Activity of Flavonoids ...

    African Journals Online (AJOL)

    Purpose: To identify the main flavonoid in Adinandra nitida leaf, employ response surface methodology to optimize its ultrasonic-assisted extraction, and determine the antioxidant activity of the obtained extract. Methods: The main flavonoid in Adinandra nitida leaf was obtained by traditional solvent extraction and ...

  1. Extraction and antioxidant activities of two species Origanum plant ...

    African Journals Online (AJOL)

    The antioxidant of ethanolic extract of two species of Origanum and essential oil of plant Origanum vulgare were investigated and also the total phenolic and flavonoid content measured. The radical scavenging activity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenolic and flavonoid ...

  2. Determination of antioxidant activity in methanolic and chloroformic ...

    African Journals Online (AJOL)

    Administrator

    2011-06-06

    Jun 6, 2011 ... The total antioxidant activity results indicated that, the inhibition percent of methanolic extract was significantly higher than the inhibition percent of chloroformic extract in the FTC and TBA methods. A higher IC50 value for free radical scavenging was found for methanolic extract when compared with ...

  3. Chemical compositions and antioxidant activity of Heracleum persicum essential oil

    Directory of Open Access Journals (Sweden)

    Maryam Gharachorloo

    2018-02-01

    Full Text Available ABSTRACT In this study essential oil of the aerial parts of Heracleum persicum a spice widely used in Iran was isolated by conventional hydrodistillation (HD and microwave-assisted hydrodistillation (MAHD techniques. The extraction yield was determined and the chemical compositions of essential oils were identified by the application of gas chromatography/mass spectrometry (GC/MS. The antioxidant activity was determined by two different methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH free radical scavenging and oven test methods. Although the main compounds of essential oils by the both extraction methods were similar, the essential oil extracted by HD with lower extraction efficiency showed more diverse compounds. The evaluation of antioxidant activity of the essential oil measured by delay in sunflower oil oxidation indicated that the antioxidant activity was dependent on the concentration which increased when higher concentrations of the essential oils were applied. The results of DPPH radical assay also indicated that the percentage of inhibition increased with increasing of essential oil concentration and IC50 value for essential oil extracted by MAHD method was obtained 1.25 mg/mL. Therefore the Heracleum persicum essential oil might be recommended for use as a flavoring agent and a source of natural antioxidants in functional foods, formulation of the supplements and in medicinal due to numerous pharmacological activities.

  4. In vitro antioxidant and free radical scavenging activity of Leonurus ...

    African Journals Online (AJOL)

    The total phenolic compounds in the extracts were determined as gallic acid equivalents, and total flavonoid contents were calculated as quercetin equivalents from a calibration curve. Keywords: Antioxidant activity, Fe2+ chelating, 1,1-diphenyl-2-picryl hydrazyl (DPPH), phenol, Leonurus cardiaca, Grammosciadium ...

  5. rich extract on total polyphenols and antioxidant activity obtained by ...

    African Journals Online (AJOL)

    Z. Ghouila

    USTHB, Organic Functional Analysis Laboratory, 16111 Bab Ezzouar, Algiers, Algeria. 3. Department of General ... Keywords: Ahmeur Bouamer, extraction, grape seeds, total polyphenols, antioxidant activity. ... in food and pharmaceutical industry like BHA and BHT, which their undesirable effect on health was often ...

  6. Antioxidant activity study and total phenolic determination of leaf ...

    African Journals Online (AJOL)

    Methodology and results: This work was used to study the antioxidant activity of two extracts of Ximenia americana (Tallow wood) by the methods of ABTS•+ and DPPH using ascorbic acid as standard. It determined the rate of total phenolic in these extracts by the Folin-Ciocalteu (FC) method using Gallic acid as standard.

  7. In vitro antioxidant and antimicrobial activities of aerial parts of ...

    African Journals Online (AJOL)

    Folin-Ciocalteu and aluminum chloride procedures were used to quantify the total phenolic and flavonoid contents, respectively, while antioxidant properties were determined by two methods: phosphomolybdenum and DPPH assays. Antimicrobial activity was also evaluated by disc diffusion method. Results: The ethyl ...

  8. Antioxidant and Antimicrobial Activities of Flavonoid Glycosides from ...

    African Journals Online (AJOL)

    Dennettia tripetala a Nigerian medicinal plant widely employed in the management of oxidative stress related diseases and infections was investigated for its active constituents. A DPPH (1,1-diphenyl-2-picrylhydrazine) guided fractionation was used to target and isolate the antioxidant constituents of the ethyl acetate ...

  9. Evaluation of proximate and antioxidant activities of Ethiopian ...

    African Journals Online (AJOL)

    ... domesticated for their leaves, fruits or both; eaten as vegetables or used in traditional medicine. However, S. aethiopicum and S. macrocarpum are the most cultivated and most utilized in Nigeria. Nutritional composition, phytochemical screening and antioxidant activity of S. aethiopicum and S. macrocarpon were studied.

  10. Evaluation of phytochemical constituents and antioxidant activity of ...

    Indian Academy of Sciences (India)

    2013-10-01

    Oct 1, 2013 ... stress (Goyal et al. 2010). Polyphenols, naturally occurring in many fruits, account for the majority of antioxidant activity (Li et al. 2012). However, polyphenols can under- go various reactions in the course of ..... ethnomedicinal species in a subtropical forest of Meghalaya, northeast India. Asian Pac. J. Trop.

  11. Improvement of nutritional quality and antioxidant activities of yeast ...

    African Journals Online (AJOL)

    This study evaluated the potential use and improves the health beneficial properties of the soybean waste manufacturing products by solid-state fermentation of six GRAS different yeast strains, including extractable antioxidant activities and bioavailable nutritional compositions. In comparison with nonfermented okara ...

  12. In vitro antioxidant activity and phytochemical screening of methanol ...

    African Journals Online (AJOL)

    Both extracts showed no significant differences (p > 0.05) in their levels of thiobarbituric acid reactive substances (TBARS). The results suggest that D. edulis and F. capensis leaves possess ample antioxidant activity and phytochemical constituents which may play important roles in the pharmaceutical and food industries.

  13. Antioxidant activities of seed extracts from Dalbergia odorifera T. Chen

    African Journals Online (AJOL)

    The heartwood or root of Dalbergia odorifera T. Chen is an important traditional Chinese medicine. Antioxidant activities of seed extracts from D. odorifera T. Chen were first investigated in this study. Ethanolic extracts were suspended in distilled water and partitioned successively with petroleum ether, ethyl acetate, ...

  14. Antimicrobial and Antioxidant Activities of the Essential Oils of Some ...

    African Journals Online (AJOL)

    Purpose: To determine the antimicrobial and antioxidant activities of Pulicaria inuloides and Ocimum forskolei essential oils. Methods: Steam distillation of the aerial parts of P. inuloides and O. forskolei was performed using a Clevenger apparatus. Essential oils were analyzed by gas chromatography–mass spectrometry.

  15. Antioxidant activity of maillard reaction products from lysine-glucose ...

    African Journals Online (AJOL)

    Maillard reaction (MR) was carried out in L-lysine-D-glucose (Lys-Glu) model system heated at 120°C for 0 to 10 h without pH control. Optical property (UV-Vis absorbance and fluorescence) development of MR was monitored. Antioxidant activity of maillard reaction products (MRPs) was investigated by a series of in vitro ...

  16. Seasonal variation in phytochemicals and antioxidant activities in ...

    African Journals Online (AJOL)

    Edoja

    2014-01-22

    Jan 22, 2014 ... Full Length Research Paper. Seasonal variation in phytochemicals and antioxidant activities in different tissues of various Broccoli cultivars. Shiva Ram Bhandari and Jung-Ho Kwak*. Vegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration,.

  17. Unintended effects were investigated in antioxidant activity between ...

    African Journals Online (AJOL)

    Other than the targeted approach on compositional analysis, non-targeted approaches on genomics, proteomics and metabolomics are developing to search for unintended effects with respect to genetically modified (GM) food safety assessments. Antioxidant activity system was closely related with plant growth and ...

  18. Antioxidant activity of the exudate from Aloe barbadensis leaves in ...

    African Journals Online (AJOL)

    The effects of the exudate of Aloe barbadensis leaves on oxidative stress and some antioxidant status of streptozotocin induced - diabetic rats were studied. There was significant reduction in scavenging enzymes like superoxide dismutase (SOD) activity and significant increase in signs of oxidative tissue damage, such as ...

  19. Retention of phenolics, carotenoids, and antioxidant activity in the ...

    African Journals Online (AJOL)

    The effects of three drying methods on phenolic and carotenoid content and antioxidant activity (AOA) of Tainong 66 cultivar sweet potato (Ipomoea batatas Lam.) were investigated. Roots of 12 batches of freshly harvested tuberous root were washed in tap water, allowed to dry, peeled and cut into small pieces of 0.5 to 1 ...

  20. Extraction and antioxidant activity of phenolic compounds from ...

    African Journals Online (AJOL)

    Purpose: To use response surface methodology to optimize the extraction of the phenolic compounds in wheat bran treated by steam explosion, and to determine the antioxidant activity of the extract obtained. Methods: By using response surface methodology, the effects of extraction time, methanol concentration, ...

  1. Antioxidant activity of nebivolol in the rat aorta

    NARCIS (Netherlands)

    de Groot, Annemieke A.; Mathy, Marie-Jeanne; van Zwieten, Pieter A.; Peters, Stephan L. M.

    2004-01-01

    The beta-blocker nebivolol is a racemic mixture Of D- and L-enantiomers that displays negative inotropic as well as direct vasorelaxant activity. In addition, it has been proposed that nebivolol exerts endothelium-protective effects caused by its antioxidant properties. In the present study we

  2. Screening of various botanical extracts for antioxidant activity using ...

    African Journals Online (AJOL)

    Aiming at the exploration of herbal use by society, crude extracts of the seeds of some commonly used medicinal plants (Vitis vinifera, Tamarindus indica and Glycin max) were screened for their free radical scavenging properties using ascorbic acid as standard antioxidant. Free radical scavenging activity was evaluated ...

  3. Composition and antioxidant and antifungal activities of the ...

    African Journals Online (AJOL)

    In this study, the oil constituents of Lippia gracilis were identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The antioxidant and antifungal activities were also evaluated. The leaf oil showed a yield of 3.7% and its main constituents were thymol (70.3%), p-cymene (9.2%), thymol ...

  4. Antioxidant activity, phenolic and flavonoid contents of some wild ...

    African Journals Online (AJOL)

    This study reported on five plants known for their use in traditional medicine in southeastern Algeria, namely: Artemisia campestris, Asteriscus pygmaeus, Pituranthos chlorantus, Pallenis spinosa and Aizoon hispanicum. The work aimed to determine the antioxidant activity, phenol and flavonoid contents of their aqueous ...

  5. total phenols and antioxidant activities of natural honeys

    African Journals Online (AJOL)

    B. S. Chandravanshi

    substance produced by honeybees (Apis mellifera) from the nectar of blossoms and secretions of plants. It is known to have both enzymatic and non-enzymatic antioxidant activities [2, 3]. Though, honey is a highly supersaturated solution of a complex mixture of sugars, it also contains small amount of other constituents ...

  6. Cytotoxic and Antioxidant Activities of Antidesma thwaitesianum Müll ...

    African Journals Online (AJOL)

    Purpose: To investigate the cytotoxic and antioxidant activities of the fruit and fruit waste (residue and marc) extracts of Antidesma thwaitesianum Müll. Arg., known as mamao in Thai, using chemical and cell-based assays. Methods: The cytotoxicity of mamao fruit and fruit waste extracts obtained by expression, maceration ...

  7. Antioxidant activity and total phenolic and flavonoid content of ...

    African Journals Online (AJOL)

    ajl yemi

    2011-12-19

    Dec 19, 2011 ... Full Length Research Paper. Antioxidant activity and total phenolic and flavonoid content of Astragalus squarrosus Bunge. Jinous Asgarpanah1*, Saeed Mohammadi Motamed2, Avishan Farzaneh1, Bahareh. Ghanizadeh1 and Simin Tomraee2. 1Young Researchers Club (YRC), Pharmaceutical Sciences ...

  8. Antioxidant activities of extracts from areca (Areca catectu L.) flower ...

    African Journals Online (AJOL)

    The antioxidant activities of areca ( Areca catectu L.) flower, husk and seed extracts were evaluated using 3 complementary in vitro assays, inhibition of DPPH (1,1-diphenyl-2- picrylhydrazyl) radicals, inhibition of hydroxyl radicals and reducing power system. The EC50 values were calculated for all the methods in order to ...

  9. Antioxidant activity and total phenolic and flavonoid content of ...

    African Journals Online (AJOL)

    The antioxidant capacity of the flowering aerial parts of Astragalus squarrosus was determined by 1,1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and ferric thiocyanate methods. The phenolic and flavonoid content was also measured. A. squarrosus showed weak free radical scavenging activity with the DPPH ...

  10. Antioxidant and Antimicrobial Activity of Polyphenol Extracts from ...

    African Journals Online (AJOL)

    Purpose: To assess the antioxidant and antimicrobial activities of polyphenolic extracts of three wild red wild berry fruit species from Southeast Serbia, viz, European cornel (Cornus mas), blackthorn (Prunus spinosa L.) and wild blackberry (Rubus fruticosus). Methods: Polyphenol content was determined using ...

  11. Antioxidant activity of the inflorescents of Vernonia calvoana ...

    African Journals Online (AJOL)

    Vernonia calvoana is an indigenous green leafy vegetable that grows around the Cameroons and the southern part of Nigeria. This study was designed to evaluate the phytochemical and antioxidant activity of V. calvoana inflorescents using the following methods: 1, 1 – diphenyl-2-picrylhydrazyl radical (DPPH), Ferric ...

  12. Antioxidant activities of Rosmarinus officinalis L. essential oil ...

    African Journals Online (AJOL)

    The essential oils of Rosmarinus officinalis L. growing in a rural area within the Nkonkobe Municipality of the Eastern Cape, South Africa, were extracted using the solvent free microwave extractor (SFME) and hydro-distillation (HD) methods. The antioxidant and free radical scavenging activity of the obtained oils were tested ...

  13. In vitro antioxidant activities of extracts of Vernonia amygdalina and ...

    African Journals Online (AJOL)

    Vernonia amygdalina and Ocimum gratissimum occupy prominent positions in the diets of several Nigerian ethnic groups. This study therefore compared the phenolic content and some antioxidative activities of the ethanolic leaf extracts of these two plants. Ethanolic extracts of freshly plucked leaves were obtained using ...

  14. Antioxidant and antimicrobial activities of solvent fractions of ...

    African Journals Online (AJOL)

    activity with standard antioxidants, gallic acid and quercetin included in the study. All fractions displayed moderate antimicrobial ..... had reported a similar finding, reporting that fungi are more resistant to plant extracts than ... The study received partial financial support from the Uni- versity of Ibadan Senate research grant ...

  15. Antioxidant activity in vitro and hepatoprotective effects in vivo of ...

    African Journals Online (AJOL)

    Background: Chinese medicine has its own uniqueness, advantageous in the treatment of hepatic diseases, and they were widely used in the oxidation. At the same time, oxidation is one of the mechanism of protect liver. Materials and Methods: In the present study, the antioxidant activity in vitro of different extracts from ...

  16. Alpha-Glucosidase Inhibitory and Antioxidant Activity of Solvent ...

    African Journals Online (AJOL)

    Alpha-Glucosidase Inhibitory and Antioxidant Activity of Solvent Extracts and Fractions of Typha domingensis (Typhaceae) Fruit. Tsun-Thai Chai, Mei-Jee Chiam, Chi-Hou Lau, Nor Ismaliza Mohd Ismail, Hean-Chooi Ong, Fazilah Abd Manan, Fai-Chu Wong ...

  17. Polyphenolic constituents and antioxidant/antiradical activity in ...

    African Journals Online (AJOL)

    Alstonia scholaris (Linn.) leaves extracted in aqueous, dichloromethane (DCM), methanolic and ethanolic solvents were assessed for different polyphenolic constituents endowed with antioxidant/antiradical activity. Total phenolic, flavonoids and tannin contents were significantly (P<0.05) higher in ethanolic extract as ...

  18. Antioxidant and antimicrobial activities of solvent fractions of ...

    African Journals Online (AJOL)

    Results: The ethyl acetate fraction having the IC50 value of 6.50 μg/mL demonstrated comparable DPPH radical-scavenging activity with standard antioxidants, gallic acid and quercetin included in the study. All fractions displayed moderate antimicrobial potential against the tested pathogens with the zone of inhibition that ...

  19. Polyphenolic constituents and antioxidant/antiradical activity in ...

    African Journals Online (AJOL)

    USER

    2015-11-25

    Nov 25, 2015 ... The crushed leaves boiled in the edible oil have been used ... polyphenolic constituents endowed with high antioxidant/ antiradical activity in different extracts of A. scholaris leaves. MATERIALS AND METHODS. Collection and ... container of soxhlet apparatus according to method described by. Harborne ...

  20. Unintended effects were investigated in antioxidant activity between ...

    African Journals Online (AJOL)

    Jane

    2011-08-22

    Aug 22, 2011 ... Other than the targeted approach on compositional analysis, non-targeted approaches on genomics, proteomics and metabolomics are developing to search for unintended effects with respect to genetically modified (GM) food safety assessments. Antioxidant activity system was closely related with.

  1. Determination of antioxidant and α-glucosidase inhibitory activities ...

    African Journals Online (AJOL)

    Antioxidant and α-glucosidase inhibitory activities as well as luteolin contents were analysed in extracts of Chrysanthemum morifolium Ramat (CM), which is a traditional medicinal plant and an important oriental herbal medicine for the treatment of eye diseases in China and Japan. In this study, the results show that the ...

  2. evaluation of antioxidant activity and polyphenolic contents of two ...

    African Journals Online (AJOL)

    A. Djouadi*, T. Lanez and C. Boubekri

    2016-05-01

    May 1, 2016 ... Polyphenolic compounds and antioxidant activity in eggplants (Solanum melongena L.) seem to depend on cultivar, ... two eggplant varieties studied, significant differences in total phenolic content were found in ... In general, the phenolic content of different parts of ethanolic extracts decreased as follows:.

  3. Antioxidant and Anti-proliferative Activities of Flavonoids from ...

    African Journals Online (AJOL)

    Purpose: To investigate the chemical composition of Bidens pilosa L. var. radiata. Sch Bip. (BP), as well as its antioxidant and anti-proliferative activities. Methods: The whole herb of BP was extracted with 95 % ethanol, which was then partitioned sequentially with petroleum ether, ethyl acetate and n-butyl alcohol to obtain ...

  4. Antioxidant activities and flavonoid contents of selected plants ...

    African Journals Online (AJOL)

    user

    2012-09-27

    Sep 27, 2012 ... The methanolic extract of three parasitic plants belonging to family Loranthaceae (Plicosepalus acacia,. Plicosepalus curviflorus and Phragmanthera austro arabica) were investigated for their antioxidant activity. The free radical and nitrogen oxide scavenging abilities were evaluated using diphenyl-1-.

  5. Cytotoxicity, Total Phenolic Contents and Antioxidant Activity of the ...

    African Journals Online (AJOL)

    The leaves of Annona muricata were extracted using ethanol and the extracts were evaluated for cytotoxicity using Brine Shrimp Lethality Assay, total phenolic content (TPC) and antioxidant activity using DPPH radical scavenging assay. The crude extract showed 73.33 % mortality at 1000 μg/mL concentration and its ...

  6. Antioxidant and Antimicrobial Activities of Ethanol Extracts of Cynara ...

    African Journals Online (AJOL)

    Erah

    benefits were due to its high fiber content [2-. 4]. The purpose of this study was to characterize antimicrobial and antioxidant activity of the freeze-dried ethanol extracts of artichoke. Another was to determine its total phenol and flavonoid contents. EXPERIMENTAL. Plant material and its extraction. The dried leaves of Cynara ...

  7. Response of total phenolic content and antioxidant activities of bush

    African Journals Online (AJOL)

    user

    the shade at ambient temperature (Mudau and. Ngezimana, 2014) before being ground with a commercial .... Saturated solution of sodium bicarbonate (20% Na2CO3,. 7.5 ml) was added to the reaction before it was ..... Effects of solvent extraction on phenolic content and antioxidant activity of the by-product of eggplant ...

  8. Effect of Heat on Antioxidant Activity of Some Tropical Leafy ...

    African Journals Online (AJOL)

    USER

    These results suggest that although thermal treatments enhances the nutritional value of vegetables by increasing the bio-accessible phenolic content and total antioxidant activity, long simmer ..... (Odukoya et al., 2007; Morales et al., 2002). Diverse groups of phenolics, being the widest spread secondary metabolite in plant ...

  9. Antioxidant and free radical scavenging activities of plant extracts ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-06-17

    Jun 17, 2008 ... Twenty-two species of medicinal plants collected in the Mexican state of Morelos were selected to evaluate their free radical scavenging and antioxidant activities. The extracts from the aerial parts of the plants were obtained using hexane, acetone and methanol (66 extracts). The initial qualitative screening.

  10. Antioxidant activities of the selected plants from the family ...

    African Journals Online (AJOL)

    USER

    2010-02-15

    Feb 15, 2010 ... were found in n-butanol extract of Ricinus communis. Antioxidant activities of these extracts were evaluated through DPPH• radical scavenging, phosphomolybdate and ferric thiocyanate (FTC) methods. Methanolic extract of Cinnamomum zeylanicum and Cinnamomum tamala showed highest antiradical.

  11. Observation of antioxidant activity of leaves, callus and suspension ...

    African Journals Online (AJOL)

    Antioxidant activity and phenolic compound was found in Justicia gendarussa via total phenolic content (TPC) and ,-diphenyl--pycrilhydrazil hydrate (DPPH) radical scavenging assays. The assays were applied on aqueous and methanolic extracts of leaves, callus culture and cell suspension culture of J. gendarussa. Callus ...

  12. Antioxidant activity of aqueous extract of a Tolypocladium sp ...

    African Journals Online (AJOL)

    Tolypocladium sp. Ts-1 was isolated from the fruiting body of a wild Cordyceps sinensis, one of the best known traditional Chinese medicine and health foods. The antioxidant activities of hot-water extracts from cultured mycelia of Tolypocladium sp. were assessed in different in vitro systems. The extracts showed ...

  13. Antioxidant activity of aqueous extract of a Tolypocladium sp. fungus ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-09-03

    Sep 3, 2008 ... Tolypocladium sp. Ts-1 was isolated from the fruiting body of a wild Cordyceps sinensis, one of the best known traditional Chinese medicine and health foods. The antioxidant activities of hot-water extracts from cultured mycelia of Tolypocladium sp. were assessed in different in vitro systems. The extracts ...

  14. Improvement of nutritional quality and antioxidant activities of yeast ...

    African Journals Online (AJOL)

    Jane

    2011-06-27

    Jun 27, 2011 ... the nutritional quality and antioxidant activities of the substrate were enhanced by solid yeast treatment fermentation. Thus, scope exists for ..... may have definite advantages in a diet. It can act as a suitable replacement ..... varying degrees, while giving a good performance in its scavenging of free radicals.

  15. Antioxidant, anti-inflammatory and antinociceptive activities of ...

    African Journals Online (AJOL)

    Background of study: Plants used for traditional medicine contain a wide range of substances which can be used to treat various infectious diseases. Aim: The study evaluated the in vitro antioxidant, antinociceptive, and anti-inflammatory activities of the methanolic extract of Justicia secunda Vahl leaf. Methods: The acute ...

  16. Fulvic acid affects pepper antioxidant activity and fruit quality

    African Journals Online (AJOL)

    standard

    2012-08-23

    Aug 23, 2012 ... analysis). Methanol extracts of freeze-dried fruits were prepared for the determination of antioxidant activity and total phenolic content. Weighed pepper fruit samples (5 g) were placed in a glass beaker and homogenised with 50 ml of methanol at. 24°C overnight. The homogenate was filtered and then.

  17. Correlation of antioxidant activity of dried berry infusions with the ...

    African Journals Online (AJOL)

    Antioxidant activity was measured by ferric reducing ability of plasma (FRAP) assay in seven types of infusions prepared from commercial dried berry fruit products: Rosa canina, Vaccinium vitis-idaea, Hiphophae rhamnoides, Hibiscus sabdariffa and three fruit mixtures. Total polyphenols (TP), total anthocyanins and the ...

  18. A novel acyclic diterpenic alcohol isolated from antioxidant active ...

    African Journals Online (AJOL)

    Background: Isolation and characterization of a new compound from the antioxidant active ethanol extract of leaves of an endemic plant Centaurothamnus maximus. Methods: The air dried powdered leaves of the plant was extracted successively with n-hexane, dichloromethane, ethyl acetate and ethanol. The obtained ...

  19. Antioxidant Activities of the Leaf Extract and Fractions of Cola ...

    African Journals Online (AJOL)

    Prof. Ogunji

    the possibility of chronic diseases such as diabetes, cancer and cardiovascular diseases (Myojin et al.,. 2008; Saha et al., 2004; Horax et al., 2005; Semiz and Sen, .... in 4 varieties of bitter melons (Momordica charantia) and antioxidant activities of their extracts. J. Food. Sci. 70(4): 275-280. Ito, N., Fukushima, S., Hagiwara, ...

  20. Application of metabolite profiling and antioxidant activity in ...

    African Journals Online (AJOL)

    This paper investigates the effect of processing and extraction method on chemical profiles and antioxidant activity of Malaysian stingless bee propolis. High Performance Thin Layer Chromatography (HPTLC) analysis in combination with chemometric shows that some of the compounds were degraded or not detected in ...

  1. Synthesis and evaluation of antioxidant and antimicrobial activities ...

    African Journals Online (AJOL)

    Purpose: To synthesize and evaluate Schiff base Tin (II) complexes for antioxidant and antimicrobial activities. Methods: The complexes of Tin (II) chloride with various Schiff base derivative of 2-Hydroxy-1- naphthaldehyde (HN) were synthesized and characterized by various physiochemical techniques, including elemental ...

  2. In vitro Antifungal, Antioxidant and Cytotoxic Activities of a Partially ...

    African Journals Online (AJOL)

    Purpose: To determine the in vitro antifungal and antioxidant activities of the aqueous extract and protein fraction of Atlantia monophylla Linn (Rutaceae) leaf. Methods: Ammonium sulphate (0 – 80 %) precipitation method was used to extract protein from the leaves of A. monophylla Linn (Rutaceae). In vitro antifungal ...

  3. In vitro callus induction and antioxidant activity of Rauwolfia ...

    African Journals Online (AJOL)

    Background: Rauwolfia vomitoria Afzel. (Apocynaceae) is a medicinal plant valued for its antipsychotic effect and used in various herbal preparations. Objectives: This study was designed to develop a protocol for callus initiation in Rauvolfia vomitoria and to investigate the antioxidant activity of the wild plant and the ...

  4. Investigation of antioxidant activity and lipophilicity parameters of some preservatives

    Energy Technology Data Exchange (ETDEWEB)

    Korotkova, E.I. [Department of Phys. Anal. Chem., Tomsk Polytechnic University, Lenin 30, Tomsk (Russian Federation) and Scientific Research Center ' Polyant' , Kulagina 5-29, Tomsk (Russian Federation)]. E-mail: eikor@mail.ru; Avramchik, O.A. [Department of Phys. Anal. Chem., Tomsk Polytechnic University, Lenin 30, Tomsk (Russian Federation); Scientific Research Center ' Polyant' , Kulagina 5-29, Tomsk (Russian Federation); Angelov, T.M. [Department of Quality Control, Unipharm-JSCo, Traiko Stanoev 3, Sofia (Bulgaria); Karbainov, Y.A. [Department of Phys. Anal. Chem., Tomsk Polytechnic University, Lenin 30, Tomsk (Russian Federation)

    2005-10-10

    The preservatives have been widely used in food, drugs and cosmetic products to prevent their aging and decay. Antioxidant and antimicrobial activities of some preservatives, such as benzoic acid, sorbic acid and alkyl-parabens, were considered in this work. Study of antioxidant properties of these compounds was carried out by differential pulse voltammetry together with evaluation of their influence on the kinetics of the electrochemical oxygen reduction. Mathematical models of the processes were obtained and their adequacy was estimated using design of experiment methods. As a result, the kinetic parameters of interaction between reactive oxygen species and preservatives were evaluation. Finally, the use of these substances as antimicrobials and antioxidants ensured product stability and safety has been recommended.

  5. Assessment of antioxidant activity in Victorian marine algal extracts using high performance thin-layer chromatography and multivariate analysis.

    Science.gov (United States)

    Agatonovic-Kustrin, Snezana; Morton, David W; Ristivojević, Petar

    2016-10-14

    The aim of this study was to develop and validate a rapid and simple high performance thin layer chromatographic (HPTLC) method to screen for antioxidant activity in algal samples. 16 algal species were collected from local Victorian beaches. Fucoxanthin, one of the most abundant marine carotenoids was quantified directly from the HPTLC plates before derivatization, while derivatization either with 2,2-diphenyl-1-picrylhydrazyl (DPPH) or ferric chloride (FeCl 3 ) was used to analyze antioxidants in marine algae, based on their ability to scavenge non biological stable free radical (DPPH) or to chelate iron ions. Principal component analysis of obtained HPTLC fingerprints has classified algae species into 5 groups according to their chemical/antioxidant profiles. The investigated brown algae samples were found to be rich in non-and moderate-polar compounds and phenolic compounds with antioxidant activity. Most of the phenolic iron chelators also have shown free radical scavenging activity. Strong positive and significant correlations between total phenolic content and DPPH radical scavenging activity showed that, phenolic compounds, including flavonoids are the main contributors of antioxidant activity in these species. The results suggest that certain brown algae possess significantly higher antioxidant potential when compared to red or green algae and could be considered for future applications in medicine, dietary supplements, cosmetics or food industries. Cystophora monilifera extract was found to have the highest antioxidant concentration, followed by Zonaria angustata, Cystophora pectinate, Codium fragile, and Cystophora pectinata. Fucoxanthin was found mainly in the brown algae species. The proposed methods provide an edge in terms of screening for antioxidants and quantification of antioxidant constituents in complex mixtures. The current application also demonstrates flexibility and versatility of a standard HPTLC system in the drug discovery. Proposed

  6. Constituents and Antioxidant Activity of Bleeding Sap from Various Xinjiang Grapes.

    Science.gov (United States)

    Le, Lv; Umar, Anwar; Iburaim, Arkin; Moore, Nicholas

    2017-10-01

    Wine grape sap or bleeding sap of grapes (GBS) is commonly used in Xinjiang (China) for therapeutic aims. Do variations in composition related to region and variety affect its properties? GBS samples originating in various parts of Xinjiang (Turpan, Hotan, Kashgar, and Atush) were tested for phenols and polyphenols, polysaccharides, saponin, proteins, individual amino acids, and minerals. Their antioxidant activity was measured using ascorbic acid as reference. Polyphenol content varied from 2.6 to 6.6 mg/L, polysaccharides 18.3-816 mg/L, saponin 6.25-106 mg/L, and protein 3.0-22.4 mg/L. Mineral elements and amino acids ranged from 6.20 to 201.2 mg/L and 0.06-118.7 mg/L, respectively. ·OH scavenging ability varied from 70% to over 90%, higher than Vitamin C. Grapes from Turpan had lower antioxidant activity than other grapes even though the polyphenol content was generally higher. Bleeding sap of Xinjiang grape is rich in amino acids, polysaccharides, polyphenols, and protein. The contents are different according to the origin, related possibly to species, climate, and environment. Antioxidant effects were not correlated with polyphenol content. Antioxidant activity of plants or plant extracts is often associated with polyphenolsBleeding sap of grapes has strong antioxidant propertiesBleeding sap from different grape varieties from different parts of Xinjiang (China) had different polyphenol concentrationsThere was no correlation of polyphenol concentrations with antioxidant activity. Abbreviations used: GBS: Bleeding sap of grapes; PITC: phenyl isothiocyanate.

  7. Antioxidative, anticholinesterase and antityrosinase activities of the ...

    African Journals Online (AJOL)

    The solvent extracts of a red alga Grateloupia lancifolia were used to evaluate biofunctional activities. The diethyl ether extract showed the highest total phenolic content and biological activities. The IC50 value of the diethyl ether extract on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was 113.8 μg/ml.

  8. Antioxidant activity of aqueous extract of noni in dilutent for ram semen cryopreservation

    Directory of Open Access Journals (Sweden)

    Ana Lauren Costa Nascimento

    2016-03-01

    Full Text Available Noni (Morinda citrifolia L. is a fruit consumed worldwide because of its nutritional and therapeutic properties resulting from the large amount of phenolic compounds, which has aroused interest of the scientific community. In order to identify new natural sources of antioxidants, the objective of this study was to evaluate the performance of noni in diluent for ram semen cryopreservation. A completely randomized design consisting of four treatments and three repetitions per treatment was used. The treatments differed in terms of the concentration of the aqueous extract of noni added to the diluent: control, no addition of the extract, and three concentrations (24, 72, and 120 µg/mL. The physical and chemical variables of the mature fruit were evaluated: total acidity (8.78, pH (4.12, and soluble solids (8.18%. The vitamin C content was 309.42 mg per 100 g fresh matter. The aqueous extract of noni was also evaluated regarding the quantity of total phenolic compounds, antioxidant activity, and lipid peroxidation inhibition capacity. The aqueous extract contained a moderate amount of phenolic compounds (47.96 ± 1.95 mg gallic acid equivalent/100 g extract. The concentrations of the aqueous extract of 72 and 120 µg/mL in diluent used for semen cryopreservation inhibited lipid peroxidation by 21.75% and 51.32%, respectively. There was no positive effect of the lowest concentration (24 µg/mL. The antioxidant activity index of noni was 33.33, corresponding to very strong antioxidant activity. The aqueous extract of noni exhibits very strong antioxidant activity and its addition to the diluent for semen cryopreservation at a concentration of 72 µg/mL is able to inhibit lipid peroxidation.

  9. Total phenolics, flavonoids, tannins and antioxidant activity of lima beans conserved in a Brazilian Genebank

    Directory of Open Access Journals (Sweden)

    Tânia da Silveira Agostini-Costa

    2015-02-01

    Full Text Available The objective of this study was to characterize for the first time polyphenols and DPPH (2-diphenyl-1-picryhydrazyl radical antioxidant activity in commonly cultivated accessions of Phaseolus lunatus from an ex situ germplasm collection maintained by Embrapa, in Brazil. Furthermore, the study aimed to detect changes in total polyphenols, total flavonoids and condensed tannin for the same accessions after regeneration in a greenhouse. The results showed the diversity of the lima bean collection for phenolic compounds, which were strongly correlated with antioxidant activity. Lima beans accessions with the highest polyphenols and antioxidant activity were those with colored seeds. Conservation through cold storage of P. lunatus seeds in a cold chamber in the germplasm collection did not necessarily affect phenolic compounds. Variations observed in values after regeneration seeds may be mainly results of biotic and abiotic factors, including not only cultivar, but also environmental conditions. This study suggests that polyphenols in the lima beans present antioxidant activity, with possible beneficial effects for human health. It was expected that the potential of this tasty legume can be also used as a functional food crop and/or as a new ingredient in gastronomy.

  10. Bioactive compounds and antioxidant activity in scalded Jalapeño pepper industrial byproduct (Capsicum annuum).

    Science.gov (United States)

    Sandoval-Castro, Claudia Jaqueline; Valdez-Morales, Maribel; Oomah, B Dave; Gutiérrez-Dorado, Roberto; Medina-Godoy, Sergio; Espinosa-Alonso, L Gabriela

    2017-06-01

    Bioactive compounds and antioxidant activity were evaluated from industrial Jalapeño pepper byproducts and simulated non processed byproducts from two Mexican states (Chihuahua and Sinaloa) to determine their value added potential as commercial food ingredients. Aqueous 80% ethanol produced about 13% of dry extract of polar compounds. Total phenolic content increased and capsaicin and dihydrocapsaicin decreased on scalding samples (80 °C, 2 min) without affecting ascorbic acid. The major phenolic compounds, rutin, epicatechin and catechin comprised 90% of the total compounds detected by HPLC of each Jalapeño pepper byproducts. ORAC analysis showed that the origin and scalding process affected the antioxidant activity which correlated strongly with capsaicin content. Although scalding decreased capsaicinoids (up to 42%), phenolic content by (up to 16%), and the antioxidant activity (variable). Jalapeño pepper byproduct is a good source of compounds with antioxidant activity, and still an attractive ingredient to develop useful innovative products with potential food/non-food applications simultaneously reducing food loss and waste.

  11. Antioxidant activity of extract from Polygonum aviculare L.

    Directory of Open Access Journals (Sweden)

    CHIN-YUAN HSU

    2006-01-01

    Full Text Available Free radicals induce numerous diseases by lipid peroxidation, protein peroxidation, and DNA damage. It has been reported that numerous plant extracts have antioxidant activities to scavenge free radicals. Whether Polygonum aviculare L. (Polygonaceae has antioxidant activity is unknown. In this study, dried Polygonum aviculare L. was extracted by ethanol, and the extract was lyophilized. The antioxidant activities of extract powder were examined by free radical scavenging assays, superoxide radical scavenging assays, lipid peroxidation assays and hydroxyl radical-induced DNA strand scission assays. The results show that the IC50 value of Polygonum aviculare L. extract is 50 µg/ml in free radical scavenging assays, 0.8 µg/ml in superoxide radical scavenging assays, and 15 µg/ml in lipid peroxidation assays, respectively. Furthermore, Polygonum aviculare L. extract has DNA protective effect in hydroxyl radical-induced DNA strand scission assays. The total phenolics and flavonoid content of extract is 677.4 ± 62.7 µg/g and 112.7 ± 13 µg/g. The results indicate that Polygonum aviculare L. extract clearly has antioxidant effects

  12. Antigenotoxic and antioxidant activities of palmarosa and citronella essential oils.

    Science.gov (United States)

    Sinha, Sonali; Biswas, Dhrubojyoti; Mukherjee, Anita

    2011-10-11

    Essential oils of palmarosa and citronella have been extensively used in ancient Indian and South-east Asian traditional medicines. These essential oils have been reported to exhibit antimicrobial, anti parasitic effects against bacteria, yeasts, filamentous fungi, and viruses. In the present study the oils were tested for their potential antigenotoxic and antioxidant properties in human lymphocyte cells. The antigenotoxic effect on human lymphocyte cells (measurement of cell viability, DNA damage) was studied using trypan blue dye exclusion test, plasmid pBR322 DNA strand scission, and comet assay. Anti-oxidant activity was evaluated by DPPH(+) free radical scavenging and lipid peroxidation assay. The essential oils showed a good antigenotoxic activity against methyl methanesulphonate (MMS) and hydrogen peroxide. In addition, a significant dose dependent antioxidant activity was observed. Our data provide evidence that support the usage of palmarosa and citronella essential oils in traditional herbal preparations. They can constitute a natural source of a new and safe antioxidant. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  13. Phenolic acids and antioxidant activity of wheat species: a review

    Directory of Open Access Journals (Sweden)

    Leváková Ľudmila

    2017-10-01

    Full Text Available Wheat (genus Triticum is considered to be an important source of polyphenols, plant secondary metabolites with numerous health-promoting effects. Many phytochemicals are responsible for the high antioxidant activity of whole grain products. However, there is a lack of information about composition of phenolic acids and their concentrations in different Triticum species. Despite the fact that the increased consumption of whole grain cereals and whole grain-based products has been closely related to reduced risk of chronic diseases, bioactive compounds found in whole grain cereals have not achieved as much attention as the bioactive compounds in vegetables and fruits. Recent studies have revealed that the content of bioactive compounds and antioxidant capacity of whole grain cereals have been regularly undervalued in the literature, because they contain more polyphenols and other phytochemicals than was reported in the past. Phenolic acids represent a large group of bioactive compounds in cereals. These compounds play a significant role in the possible positive effects of the human diet rich in whole grain cereals, especially in wheat and provide health benefits associated with demonstrably diminished risk of chronic disease development. Ferulic acid, the primary and the most abundant phenolic acid contained in wheat grain, is mainly responsible for the antioxidant activity of wheat, particularly bran fraction. In this paper, selected phenolic compounds in wheat, their antioxidant activity and health benefits related to consumption of whole grain cereals are reviewed.

  14. Anticancer and Antioxidant Activity of Bread Enriched with Broccoli Sprouts

    Science.gov (United States)

    Gawlik-Dziki, Urszula; Świeca, Michał; Dziki, Dariusz; Sęczyk, Łukasz; Złotek, Urszula; Różyło, Renata; Kaszuba, Kinga; Ryszawy, Damian; Czyż, Jarosław

    2014-01-01

    This study is focused on antioxidant and anticancer capacity of bread enriched with broccoli sprouts (BS) in the light of their potential bioaccessibility and bioavailability. Generally, bread supplementation elevated antioxidant potential of product (both nonenzymatic and enzymatic antioxidant capacities); however, the increase was not correlated with the percent of BS. A replacement up to 2% of BS gives satisfactory overall consumers acceptability and desirable elevation of antioxidant potential. High activity was especially found for extracts obtained after simulated digestion, which allows assuming their protective effect for upper gastrointestinal tract; thus, the anticancer activity against human stomach cancer cells (AGS) was evaluated. A prominent cytostatic response paralleled by the inhibition of AGS motility in the presence of potentially mastication-extractable phytochemicals indicates that phenolic compounds of BS retain their biological activity in bread. Importantly, the efficient phenolics concentration was about 12 μM for buffer extract, 13 μM for extracts after digestion in vitro, and 7 μM for extract after absorption in vitro. Our data confirm chemopreventive potential of bread enriched with BS and indicate that BS comprise valuable food supplement for stomach cancer chemoprevention. PMID:25050366

  15. Fatty Acids, Antioxidants and Physical Activity in Brain Aging

    Directory of Open Access Journals (Sweden)

    Hércules Rezende Freitas

    2017-11-01

    Full Text Available Polyunsaturated fatty acids and antioxidants are important mediators in the central nervous system. Lipid derivatives may control the production of proinflammatory agents and regulate NF-κB activity, microglial activation, and fatty acid oxidation; on the other hand, antioxidants, such as glutathione and ascorbate, have been shown to signal through transmitter receptors and protect against acute and chronic oxidative stress, modulating the activity of different signaling pathways. Several authors have investigated the role of these nutrients in the brains of the young and the aged in degenerative diseases such as Alzheimer’s and Parkinson’s, and during brain aging due to adiposity- and physical inactivity-mediated metabolic disturbances, chronic inflammation, and oxidative stress. Through a literature review, we aimed to highlight recent data on the role of adiposity, fatty acids, antioxidants, and physical inactivity in the pathophysiology of the brain and in the molecular mechanisms of senescence. Data indicate the complexity and necessity of endogenous/dietary antioxidants for the maintenance of redox status and the control of neuroglial signaling under stress. Recent studies also indicate that omega-3 and -6 fatty acids act in a competitive manner to generate mediators for energy metabolism, influencing feeding behavior, neural plasticity, and memory during aging. Finding pharmacological or dietary resources that mitigate or prevent neurodegenerative affections continues to be a great challenge and requires additional effort from researchers, clinicians, and nutritionists in the field.

  16. Anticancer and antioxidant activity of bread enriched with broccoli sprouts.

    Science.gov (United States)

    Gawlik-Dziki, Urszula; Świeca, Michał; Dziki, Dariusz; Sęczyk, Łukasz; Złotek, Urszula; Różyło, Renata; Kaszuba, Kinga; Ryszawy, Damian; Czyż, Jarosław

    2014-01-01

    This study is focused on antioxidant and anticancer capacity of bread enriched with broccoli sprouts (BS) in the light of their potential bioaccessibility and bioavailability. Generally, bread supplementation elevated antioxidant potential of product (both nonenzymatic and enzymatic antioxidant capacities); however, the increase was not correlated with the percent of BS. A replacement up to 2% of BS gives satisfactory overall consumers acceptability and desirable elevation of antioxidant potential. High activity was especially found for extracts obtained after simulated digestion, which allows assuming their protective effect for upper gastrointestinal tract; thus, the anticancer activity against human stomach cancer cells (AGS) was evaluated. A prominent cytostatic response paralleled by the inhibition of AGS motility in the presence of potentially mastication-extractable phytochemicals indicates that phenolic compounds of BS retain their biological activity in bread. Importantly, the efficient phenolics concentration was about 12 μM for buffer extract, 13 μM for extracts after digestion in vitro, and 7 μM for extract after absorption in vitro. Our data confirm chemopreventive potential of bread enriched with BS and indicate that BS comprise valuable food supplement for stomach cancer chemoprevention.

  17. Volatile compounds and antioxidative activity of Porophyllum tagetoides extracts.

    Science.gov (United States)

    Jimenez, M; Guzman, A P; Azuara, E; Garcia, O; Mendoza, M R; Beristain, C I

    2012-03-01

    Porophyllum tagetoides is an annual warm-weather herb that has an intense typical smell. Its leaves are commonly used in soup preparation and traditional medicine for treatment of inflammatory diseases. Its volatile compounds and antioxidant properties were evaluated in crude, aqueous and ethanol leaf extract and an oil emulsion using different antioxidant assays in vitro, such as: DPPH radical scavenging activity, redox potential, polyphenol content, reducing power and optical density. A high antioxidative activity was found when comparing leaves with stems. The crude extract from leaves showed a very high reducing power (2.88 ± 0.20 O.D.) and DPPH radical-scavenging activity (54.63 ± 4.80%), in concordance with a major concentration of vitamin C (23.97 ± 0.36 mg/100 g). Instead, the highest polyphenol content (264.54 ± 2.17 mg GAE/g of sample) and redox potential (561.23 ± 0.15 mV) were found by the ethanol and aqueous extract, respectively. Aldehydes and terpenes such as nonanal, decanal, trans-pineno, β-myrcene and D-limonene were the major volatiles found. This study suggests that Porophyllum tagetoides extracts could be used as antioxidants.

  18. Anticancer and Antioxidant Activity of Bread Enriched with Broccoli Sprouts

    Directory of Open Access Journals (Sweden)

    Urszula Gawlik-Dziki

    2014-01-01

    Full Text Available This study is focused on antioxidant and anticancer capacity of bread enriched with broccoli sprouts (BS in the light of their potential bioaccessibility and bioavailability. Generally, bread supplementation elevated antioxidant potential of product (both nonenzymatic and enzymatic antioxidant capacities; however, the increase was not correlated with the percent of BS. A replacement up to 2% of BS gives satisfactory overall consumers acceptability and desirable elevation of antioxidant potential. High activity was especially found for extracts obtained after simulated digestion, which allows assuming their protective effect for upper gastrointestinal tract; thus, the anticancer activity against human stomach cancer cells (AGS was evaluated. A prominent cytostatic response paralleled by the inhibition of AGS motility in the presence of potentially mastication-extractable phytochemicals indicates that phenolic compounds of BS retain their biological activity in bread. Importantly, the efficient phenolics concentration was about 12 μM for buffer extract, 13 μM for extracts after digestion in vitro, and 7 μM for extract after absorption in vitro. Our data confirm chemopreventive potential of bread enriched with BS and indicate that BS comprise valuable food supplement for stomach cancer chemoprevention.

  19. Aldose reductase inhibitory activity and antioxidant capacity of pomegranate extracts.

    Science.gov (United States)

    Karasu, Cimen; Cumaoğlu, Ahmet; Gürpinar, Ali Rifat; Kartal, Murat; Kovacikova, Lucia; Milackova, Ivana; Stefek, Milan

    2012-03-01

    The pomegranate, Punica granatum L., has been the subject of current interest as a medicinal agent with wide-ranging therapeutic indications. In the present study, pomegranate ethanolic seed and hull extracts were tested, in comparison with a commercial sample, for the inhibition of aldose reductase, an enzyme involved in the etiology of diabetic complications. In vitro inhibition of rat lens aldose reductase was determined by a conventional method. Pomegranate ethanolic hull extract and commercial pomegranate hull extract exhibited similar aldose reductase inhibitory activity characterized by IC(50) values ranging from 3 to 33.3 μg/ml. They were more effective than pomegranate ethanolic seed extract with IC(50) ranging from 33.3 to 333 μg/ml. Antioxidant action of the novel compounds was documented in a DPPH test and in a liposomal membrane model, oxidatively stressed by peroxyl radicals. All the plant extracts showed considerable antioxidant potential in the DPPH assay. Pomegranate ethanolic hull extract and commercial pomegranate hull extract executed similar protective effects on peroxidatively damaged liposomal membranes characterized by 10ethanolic seed extract showed significantly lower antioxidant activity compared to both hull extracts studied. Pomegranate extracts are thus presented as bifunctional agents combining aldose reductase inhibitory action with antioxidant activity and with potential therapeutic use in prevention of diabetic complications.

  20. Total antioxidant activity of yacon tubers cultivated in Brazil

    Directory of Open Access Journals (Sweden)

    Juciane Abreu Ribeiro Pereira

    Full Text Available ABSTRACT Yacon (Smallanthus sonchifolius is a tuberous root from the Andean region in the South America rich em water, fructooligosaccharides and phenolic compounds, some of which are natural antioxidants and may help prevent the deleterious action of free radicals in the body. The yacon has attracted much attention due to their potential health benefits to humans. In this study the levels of total phenolics, tannins, phenolic acids, and total antioxidant activity were measured in the peel and pulp of yacon tubers both in the fresh and flour forms. The flours of yacon presented higher concentrations of total phenolics and tannins, especially peel flour. The yacon pulp flour stood out as the main source of phenolic acids, mainly caffeic and chlorogenic acid. The total antioxidant activity assessed by DPPH (2,2-Diphenyl-1-picrylhydrazyl and ABTS (2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid assays was higher in the yacon peel flour. The total antioxidant activity was correlated with the total phenolic content and tannins by the DPPH and ABTS assays. These results suggest that yacon can be used as an alternative food source of phenolic compounds that help prevent degenerative processes caused by oxidative stress, especially in the flours form.

  1. Antioxidant activity of the probiotic consortium in vitro

    Directory of Open Access Journals (Sweden)

    Saule Saduakhasova

    2014-01-01

    Full Text Available Introduction: Available evidence suggests that probiotics have different biological functions that depend on several mechanisms, such as antioxidant and DNA-protective activities. The probiotic consortium includes bacterial cultures such as Streptococcus thermophilus, Lactococcus lactis, Lactobacillus plantarum, and other bacterial cultures isolated from traditional Kazakh dairy products (ayran, kumys, shubat, and healthy clinical material. The aim of this study was to investigate the total antioxidant activity of the consortium of probiotic bacteria and to determine the activity of superoxide dismutase, glutathione reductase, and DNA-protective action. Material and methods: In vitro comet assay was used to determine the antigenotoxicity of the probiotic consortium. Total antioxidant activity was determined using a method of analysis with Trolox as the equivalent. The analysis method of superoxide dismutase activity assesses the inhibition rate of the nitroblue tetrazolium reduction to formazan by superoxide dismutase. Determination of glutathione reductase activity is based on the measurement of the NADPH oxidation speed. Results: A significantly high level of the total antioxidant activity of the probiotic consortium intact cells (15.3 mM/ml was observed whereas the activity index of  lysate  was 11.1 mM/ml. The superoxide dismutase activity of probiotic consortium lysate was evaluated, with values that peaked at 0.24 U/mg protein. The superoxide dismutase activity of the consortium was lower in comparison to L.fernentum E-3 and L.fernentum E-18 cultures with values of 0.85 U/mg and 0.76 U/mg protein, respectively. SOD activity of probiotic consortium whole cells was not observed, which is typical for lactic acid bacteria. Glutathione reductase plays an important role in the optimal protection from oxidative stress. Glutathione reductase activity of the studied probiotic consortium was low; moreover, the activity of the lysate was two times

  2. Antioxidant activity and irritation property of venoms from Apis species.

    Science.gov (United States)

    Somwongin, Suvimol; Chantawannakul, Panuwan; Chaiyana, Wantida

    2018-04-01

    Pharmacological effects of bee venom has been reported, however, it has been restricted to the bee venom collected from European honey bee (Apis mellifera). The aim of the present study was to compare the antioxidant activities and irritation properties of venoms collected from four different Apis species in Thailand, which includes Apis cerena (Asian cavity nesting honeybee), Apis florea (dwarf honeybee), Apis dorsata (giant honeybee), and A. mellifera. Melittin content of each bee venom extracts was investigated by using high-performance liquid chromatography. Ferric reducing antioxidant power, 2, 2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid), and 1, 1-diphenyl-2-picrylhydrazyl assay were used to determine the antioxidant activity, whereas, hen's egg test chorioallantoic membrane assay was used to determine the irritation property of each bee venom extracts. Melittin was the major constituent in all bee venom extracts. The melittin content in A. dorsata, A. mellifera, A. florea, and A. cerena were 95.8 ± 3.2%, 76.5 ± 1.9%, 66.3 ± 8.6%, and 56.8 ± 1.8%, respectively. Bee venom extract from A. dorsata possessed the highest antioxidant activity with the inhibition of 41.1 ± 2.2% against DPPH, Trolox equivalent antioxidant capacity of 10.21 ± 0.74 mM Trolox/mg and equivalent concentration (EC 1 ) of 0.35 ± 0.02 mM FeSO 4 /mg. Bee venom extract from A. mellifera exhibited the highest irritation, followed by A. cerena, A. dorsata, and A. florea, respectively. Melittin was the compound responsible for the irritation property of bee venom extracts since it could induce severe irritation (irritation score was 13.7 ± 0.5, at the concentration of 2 mg/ml). The extract from A. dorsata which possessed the highest antioxidant activity showed no irritation up to the concentration of 0.1 mg/ml. Therefore, bee venom extract from A. dorsata at the concentration not more than 0.1 mg/ml would be suggested for using

  3. Phytochemical analysis and antioxidant activities of Trigona Apicalis propolis extract

    Science.gov (United States)

    Rosli, Nur Liyana; Roslan, Husniyati; Omar, Eshaifol Azam; Mokhtar, Norehan; Hapit, Nor Hussaini Abdul; Asem, Nornaimah

    2016-12-01

    Propolis is a resinous substance found in beehives. It provides beneficial effects on human health and has been used to treat many diseases since ancient times. The objectives of this study were to analyze the phytochemical profile of propolis derived from local T. apicalis species and its antioxidant activities. The ethanolic extract of propolis was subjected to HPLC analysis to analyze its phytochemical profile. The propolis extract was later tested for antioxidant capacities by using DPPH radical scavenging assay. TPC and TFC were performed to determine the correlation with its antioxidant activities. TEAC for each serial dilution sample was 2621.15 (4.76 mg/mL), 2050.85 (2.38 mg/mL), 1883.27 (1.19 mg/mL), 1562.67 (0.59 mg/mL), 1327.82 (0.29 mg/mL), 1164.49 (0.15 mg/mL), 983.27 (0.07 mg/mL), and 944.79 (0.04 mg/mL). The results demonstrated that the antioxidant activities of propolis extract were dose dependent. The IC50 of propolis for DPPH assay was 4.27 mg/ml. Correlation values of TPC and TFC against DPPH indicate that the antioxidant activities of propolis extract used in this study could be mainly influenced by the phenolic and flavonoid contents. These findings highlighted the importance of quality analysis in order to ensure the consistency of biological effects or therapy of a natural product, such as propolis.

  4. Essential oil of Psidium cattleianum leaves: antioxidant and antifungal activity.

    Science.gov (United States)

    Castro, Micheli R; Victoria, Francine N; Oliveira, Daniela H; Jacob, Raquel G; Savegnago, Lucielli; Alves, Diego

    2015-02-01

    Psidium cattleianum Sabine (Myrtacea) is rich in vitamin C and phenolic compounds, including epicatechin and gallic acid as the main components. To evaluate the antifungal and antioxidant capacity in vitro of the essential oil of araçá (EOA). The acute toxicity of the EOA also was evaluated in mice. The leaves of the P. cattleianum were extracted by steam distillation. The antioxidant capacity was evaluated by in vitro tests [1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS), ferric ion reducing antioxidant power (FRAP), linoleic acid oxidation, thiobarbituric acid reactive species (TBARS)], and ex vivo analysis [TBARS, δ-aminulevunilate dehydratase (δ-Ala-D) and catalase activity, non-protein thiols (NPSH), and ascorbic acid levels]. The toxicity was studied in mice by a single oral administration of EOA; and the antifungal activity was performed with five strains of fungi. The EOA exhibited antioxidant activity in the FRAP assay and reduced lipid peroxidation in the cortex (Imax = 32.90 ± 2.62%), hippocampus (IC50 = 48.00 ± 3.00 µg/ml and Imax = 32.90 ± 2.62%), and cerebellum (Imax = 45.40 ± 14.04%) of mice. Acute administration of the EOA by the oral route did not cause toxicological effects in mice (LD50 > 500 µg/ml). The EOA also showed antifungal activity through of the determination minimum inhibitory concentration (MIC) values ranging from 41.67 ± 18.04 to 166.70 ± 72.17 µg/ml for tested strains. The results of present study indicate that EOA possess antioxidant properties, antifungal and not cause toxicity at tested doses.

  5. Antioxidant and Cytotoxic Activities of Centella asiatica (L) Urb.

    Science.gov (United States)

    Pittella, Frederico; Dutra, Rafael C.; Junior, Dalton D.; Lopes, Miriam T. P.; Barbosa, Nádia R.

    2009-01-01

    In the present study, the phenolic (Folin-Dennis) and flavonoid (colorimetric assay) constituents, antioxidant [2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay] and cytotoxic activities of an aqueous extract (AE) of Centella asiatica leaves were investigated. The aqueous extract (50 g/L) was obtained by infusion followed by cold maceration for 24 h. The levels of phenolic and flavonoid compounds were 2.86 g/100 g and 0.361 g/100 g, respectively. The AE showed elevated DPPH scavenging activity, with an IC50 value of 31.25 μg/mL. The AE had a promising activity against mouse melanoma (B16F1), human breast cancer (MDA MB-231) and rat glioma (C6) cell lines, with IC50 values of 698.0, 648.0 and 1000.0 μg/mL, respectively. A positive correlation was established between the level of flavonoids, antioxidant and antitumor activities. PMID:19865514

  6. Antioxidant and Cytotoxic Activities of Centella asiatica (L Urb.

    Directory of Open Access Journals (Sweden)

    Frederico Pittella

    2009-08-01

    Full Text Available In the present study, the phenolic (Folin-Dennis and flavonoid (colorimetric assay constituents, antioxidant [2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH assay] and cytotoxic activities of an aqueous extract (AE of Centella asiatica leaves were investigated. The aqueous extract (50 g/L was obtained by infusion followed by cold maceration for 24 h. The levels of phenolic and flavonoid compounds were 2.86 g/100 g and 0.361 g/100 g, respectively. The AE showed elevated DPPH scavenging activity, with an IC50 value of 31.25 μg/mL. The AE had a promising activity against mouse melanoma (B16F1, human breast cancer (MDA MB-231 and rat glioma (C6 cell lines, with IC50 values of 698.0, 648.0 and 1000.0 μg/mL, respectively. A positive correlation was established between the level of flavonoids, antioxidant and antitumor activities.

  7. Mitochondrial respiratory and antioxidative enzyme activities in turkey meat.

    Science.gov (United States)

    Janisch, S; Wicke, M; Krischek, C

    2012-02-01

    Meat quality and (anti)oxidative metabolism of m. pectoralis superficialis (MPS), m. gastrocnemius (MG) and m. iliotibilialis lateralis (MIL) from turkey toms were analysed. After slaughter, pH of MPS and MG decreased and electrical conductivity of the MPS increased. The MG had generally higher pH values. The meat lightness (L) and redness (a) increased in MG and MPS after slaughter. The MPS always had higher L and lower a values. Mitochondrial respiratory activities (MRA) were higher in the MIL than the MPS. The activities of superoxide dismutase (SOD) and glutathione peroxidase, analysed in the MPS, increased and the glutathione reductase activity decreased after slaughter. Meat samples with lower pH24 h p.m. had higher drip loss and L values. The MRA were tendentially lower and the SOD activities higher in these samples. These results indicate a relation between the meat quality, the antioxidative metabolism and mitochondrial respiration.

  8. Purification, structural characterization, and antioxidant activity of antioxidant substance from the red seaweed Gloiopeltis tenax.

    Science.gov (United States)

    Lim, Byung-Lak; Ryu, Il-Hwan

    2009-04-01

    An antioxidant substance (AOS) obtained from an enzymatic extract of the red seaweed Gloiopeltis tenax was purified by DEAE-Sephadex CL-6B and Sephadex G-100 column chromatography. The purification yield of AOS was 14.3%. The AOS predominantly contained fucose, mannose, and galactose but also contained a sulfate group. The structure of AOS was investigated by periodate oxidation, desulfation, Fourier transform-infrared spectroscopy, and nuclear magnetic resonance spectroscopy. AOS was mainly composed of alternating units of beta-D-Glc(1 --> 2)alpha-D-Man(1 --> 4)beta-D-Gal(1 --> 4)alpha-D-Man(1 --> 4)beta-D-Gal alpha-D-Man (1 --> 4) beta-D-Glc (or Xyl)- and branched linkage of alpha-D-Man(1 --> 3) alpha-D-Fuc. In addition, the fucose residues were shown to be 2-O- and 4-O-sulfated and, therefore, were either terminal or 3-linked. The antioxidative activity of AOS was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the beta-carotene-linoleate assay systems and was compared with those of butylated hydroxytoluene and ascorbic acid (AscA). The results showed that AOS exhibited higher antioxidative activity than AscA in the DPPH assay model and in the beta-carotene-linoleate assay system at all of the four concentration levels tested (from 50 to 200 microg/mL). These results suggested that AOS from the red seaweed G. tenax is an efficient novel antioxidant.

  9. In Vitro Antioxidant versus Metal Ion Chelating Properties of Flavonoids: A Structure-Activity Investigation

    Science.gov (United States)

    Cherrak, Sabri Ahmed; Mokhtari-Soulimane, Nassima; Berroukeche, Farid; Bensenane, Bachir; Cherbonnel, Angéline; Merzouk, Hafida; Elhabiri, Mourad

    2016-01-01

    Natural flavonoids such as quercetin, (+)catechin and rutin as well as four methoxylated derivatives of quercetin used as models were investigated to elucidate their impact on the oxidant and antioxidant status of human red blood cells (RBCs). The impact of these compounds against metal toxicity was studied as well as their antiradical activities with DPPH assay. Antihemolytic experiments were conducted on quercetin, (+)catechin and rutin with excess of Fe, Cu and Zn (400 μM), and the oxidant (malondialdehyde, carbonyl proteins) and antioxidant (reduced glutathione, catalase activity) markers were evaluated. The results showed that Fe and Zn have the highest prooxidant effect (37 and 33% of hemolysis, respectively). Quercetin, rutin and (+)catechin exhibited strong antioxidant properties toward Fe, but this effect was decreased with respect to Zn ions. However, the Cu showed a weak antioxidant effect at the highest flavonoid concentration (200 μM), while a prooxidant effect was observed at the lowest flavonoid concentration (100 μM). These results are in agreement with the physico-chemical and antiradical data which demonstrated that binding of the metal ions (for FeNTA: (+)Catechin, KLFeNTA = 1.6(1) × 106 M-1 > Rutin, KLFeNTA = 2.0(9) × 105 M-1 > Quercetin, KLFeNTA = 1.0(7) × 105 M-1 > Q35OH, KLFeNTA = 6.3(8.7) × 104 M-1 > Quercetin3’4’OH and Quercetin 3OH, KLFeNTA ~ 2 × 104 M-1) reflects the (anti)oxidant status of the RBCs. This study reveals that flavonoids have both prooxidant and antioxidant activity depending on the nature and concentration of the flavonoids and metal ions. PMID:27788249

  10. Antibacterial and antioxidant activities of Origanum compactum ...

    African Journals Online (AJOL)

    The oil was investigated for its in vitro antibacterial activity against a panel of standard reference strains using well diffusion and broth dilution methods. In solid medium, the oil was found to be remarkably active against all tested strains except Pseudomonas which showed resistance. In liquid medium the Minimum Inhibitory ...

  11. Antioxidant and antimicrobial activities of callus culture and leaf ...

    African Journals Online (AJOL)

    The callus culture extract (CCE) gave the lowest MIC value of 0.78 mg/ mL for most of the bacteria and fungi and the lowest MBC values of 0.78 mg/ mL and 1.56 mg/ mL against bacteria and fungi, respectively. ... Keywords: Crotalaria retusa; In vitro propagation; Callus culture; Antimicrobial activity; Antioxidant activity ...

  12. Statistical relationship of strong earthquakes with planetary geomagnetic field activity

    Science.gov (United States)

    Pogrebnikov, M. M.; Komarovski, N. I.; Kopytenko, Y. A.; Pushel, A. P.

    1984-12-01

    Earlier studies reported a significant decrease in the geomagnetic field before strong earthquakes. Possible relationships between earthquakes with magnitude greater than 7 (Soviet scale) and planetary terrestrial magnetic field activity as characterized by the K sub p index were investigated. A total of 100 cases of strong earthquakes on magnetically quiet days in 1965 to 1975 were studied. The K sub p indexes were studied for two days before and two days after the earthquakes. The dispersion curve shows a significant decrease one day before each event. The relationship of the planetary K sub p index with seismic activity indicates that the period of preparation for an earthquake and at the moment of the shock are reflected in the terrestrial magnetic field.

  13. Antioxidant activity of alstonia Angustifolia ethanolic leaf extract

    Science.gov (United States)

    Rahim, Nurhidayah Ab; Zakaria, Noorzafiza; Dzulkarnain, Syarifah Masyitah Habib; Azahar, Nazar Mohd Zabadi Mohd; Abdulla, Mahmood Ameen

    2017-10-01

    In current study, the ability of the ethanolic extract of Alstonia angustifolia in scavenging free radicals was assessed by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and hydrogen peroxide (H2O2) radical scavenging assay. The results suggested that the ethanolic extract of A. angustifolia leaves has a notable antioxidant activity. In FRAP assay, it showed that the extract have higher total antioxidant activity with FRAP value is 1868.33 µM/g Fe (ii) dry mass ± 0.15 than the control, quercetin with FRAP value is 1336.9 µM/g Fe (II) dry mass ± 0.12 and ascorbic acid with FRAP value is 1720 µM/g Fe (II) dry mass ± 0.02. For DPPH assay, the IC50 value of the extract is 384.77 while the IC50 value of standards of ascorbic acid and quercetin are 18.07 µg/ml and 39.60 µg/ml, respectively. For H2O2 scavenging assay, the IC50 value for the extract was discovered to be 186.77 µg/ml compared to standard ascorbic acid 466.56 µg/ml. Thus, the study suggests that A. angustifolia ethanolic leaf extract has a good origin of natural antioxidants and might be beneficial in impeding the oxidative stress progression thus averting diseases that related to free radicals.

  14. Hydrophilic and lipophilic antioxidant activities of guava fruits.

    Science.gov (United States)

    Thaipong, Kriengsak; Boonprakob, Unaroj; Cisneros-Zevallos, Luis; Byrne, David H

    2005-01-01

    The objectives of this study were to evaluate the hydrophilic antioxidant activity (AOAH) and the lipophilic antioxidant activity (AOAL); and their correlations with vitamin C, and total phenolic and beta-carotene contents in fresh guava fruits of one white flesh clone ('Allahabad Safeda') and three pink flesh clones ('Fan Retief', 'Ruby Supreme,' and an advanced selection). A ferric reducing antioxidant power assay was used to estimate both AOAH and AOAL from methanol and dichloromethane extracts, respectively. The white flesh clone, 'Allahabad Safeda,' showed higher levels of both AOAH [33.3 microM Trolox equivalents (TE)/g fresh weight (FW)] and AOAL (0.25 microM TE/g FW) than the pink flesh clones that ranged from 15.5 to 30.4 and from 0.12 to 0.13 microM TE/g FW for AOAH and AOAL, respectively. The AOAH was positively correlated with vitamin C (r = 0.92, p < 0.01) and total phenolic (r = 0.97, p < 0.01) but was negatively correlated with beta-carotene (r = -0.73, p = 0.03). The AOAL was not correlated with these antioxidants.

  15. Antioxidant activity of oregano essential oil (Origanum vulgare L.

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    Stanojević, Lj.P.

    2016-12-01

    Full Text Available Essential oil obtained from oregano (Origanum vulgare L. by Clevenger-type hydrodistillation and hydromodulus 1:10 m/v during 180 minutes, has been investigated in this work. Qualitative and quantitative composition of the oil was determined by GC-MS and GC-FID spectrometry. Antioxidant activity of the obtained oil was examined spectrophotometrically by DPPH test (after 20, 30, 45 and 60 minutes of incubation and TBA-MDA assay. The yield of essential oil was 4.1 mL/100 g of plant material. Seven components were identified: α-thujene, myrcene, α-terpinene, o-cymene, γ-terpinene, thymol and carvacrol. The major components were thymol (45% and carvacrol (37.4%. Oil incubated for 60 minutes has shown the best antioxidant activity according to DPPH test. The concentrations of essential oil, required for neutralization of 50% of initial DPPH radical concentration (EC50, were 0.761, 0.590, 0.360 and 0.326 mg/mL, after 20, 30, 45 and 60 minutes of incubation, respectively. Lipid peroxidation inhibition of 92.3% was achieved by 1.35 mg/mL essential oil concentration. The results obtained indicate that oregano essential oil is a good source of natural antioxidants with potential application in food and pharmaceutical industries, as a safer alternative to the synthetic antioxidants.

  16. Antioxidant Activities of Total Phenols of Prunella vulgaris L. in Vitro and in Tumor-bearing Mice

    Directory of Open Access Journals (Sweden)

    Feng Shi

    2010-12-01

    Full Text Available Prunella vulgaris L. (PV, Labiatae is known as a self-heal herb. The different extracts of dried spikes were studied for the best antioxidant active compounds. The 60% ethanol extract (P-60 showed strong antioxidant activity based on the results of 2,2’-azino-di(3-ethylbenzthiazoline-6-sulfonic acid (ABTS˙+, 2,2-diphenyl-1-picrylhydrazyl (DPPH and ferric reducing antioxidant power (FRAP assay methods. High performance liquid chromatography (HPLC and LC/MS analysis showed that the main active compounds in P-60 were phenols, such as caffeic acid, rosmarinic acid, rutin and quercetin. Total phenols were highly correlated with the antioxidant activity (R2 = 0.9988 in ABTS˙+; 0.6284 in DPPH and 0.9673 FRAP tests. P-60 could inhibit significantly the tumor growth in C57BL/6 mice. It can also been showed that increased superoxide dismutase (SOD activity and decreased malondialdehyde (MDA content in serum of tumor-bearing mice. These results suggested that P-60 extract had high antioxidant activity in vitro and in vivo and total phenols played an important role in antioxidant activity for inhibition of tumor growth.

  17. Angiotensin-I Converting Enzyme (ACE Inhibitory and Anti-Oxidant Activities of Sea Cucumber (Actinopyga lecanora Hydrolysates

    Directory of Open Access Journals (Sweden)

    Raheleh Ghanbari

    2015-12-01

    Full Text Available In recent years, food protein-derived hydrolysates have received considerable attention because of their numerous health benefits. Amongst the hydrolysates, those with anti-hypertensive and anti-oxidative activities are receiving special attention as both activities can play significant roles in preventing cardiovascular diseases. The present study investigated the angiotensin-I converting enzyme (ACE inhibitory and anti-oxidative activities of Actinopyga lecanora (A. lecanora hydrolysates, which had been prepared by alcalase, papain, bromelain, flavourzyme, pepsin, and trypsin under their optimum conditions. The alcalase hydrolysate showed the highest ACE inhibitory activity (69.8% after 8 h of hydrolysis while the highest anti-oxidative activities measured by 2,2-diphenyl 1-1-picrylhydrazyl radical scavenging (DPPH (56.00% and ferrous ion-chelating (FIC (59.00% methods were exhibited after 24 h and 8 h of hydrolysis, respectively. The ACE-inhibitory and anti-oxidative activities displayed dose-dependent trends, and increased with increasing protein hydrolysate concentrations. Moreover, strong positive correlations between angiotensin-I converting enzyme (ACE inhibitory and anti-oxidative activities were also observed. This study indicates that A. lecanora hydrolysate can be exploited as a source of functional food owing to its anti-oxidant as well as anti-hypertension functions.

  18. Anti hypoxic and antioxidant activity of Hibiscus esculentus seeds

    Energy Technology Data Exchange (ETDEWEB)

    Ebrahimzadeh, M. A.; Nabavi, S. F.; Nabavi, S. M.; Eslami, B.

    2010-07-01

    The anti hypoxic and antioxidant activities of Hibiscus esculentus seeds were investigated employing eight in vitro assay systems. Anti hypoxic activity was investigated in two models, haemic and circulatory. The effects were pronounced in both models of hypoxia. The anti hypoxic effects were dose-dependent. The results indicated that the extracts have a protective effect against hypoxia induced lethality in mice. The extracts showed antioxidant activity in some models. IC{sub 5}0 for DPPH radical-scavenging activity was 234 {+-} 8.9 {mu}g ml{sup 1}. The extracts showed weak nitric oxide-scavenging activity between 0.1 and 1.6 mg ml{sup -}1. The extracts showed weak Fe{sup 2}+ chelating ability. IC{sub 5}0 were 150 {+-} 13 {mu}g ml{sup -}1. The extracts also exhibited low antioxidant activity in the linoleic acid model but were capable of scavenging hydrogen peroxide in a concentration dependent manner. The total amount of phenolic compounds in each extract was determined as gallic acid equivalents and total flavonoid contents were calculated as quercetin equivalents from a calibration curve. Pharmacological effects may be attributed, at least in part, to the presence of phenols and flavonoids in the extracts. (Author) 40 refs.

  19. Assessment of red onion on antioxidant activity in rat.

    Science.gov (United States)

    Lee, Bora; Jung, Ji-Hye; Kim, Hyun-Sook

    2012-11-01

    Oxidative stress related to the aging process can increase the risk of degenerative disease. Red onions contain antioxidative compounds. This study was designed to investigate the effect of dietary red onion peel and/or flesh on antioxidative activity in rats. Twenty Sprague-Dawley male rats (18 weeks old) were divided into four groups. Each group was raised for 4 weeks on a red onion free control diet (ND), red onion diet containing 5% red onion peel (RP), 5% red onion flesh (RF), or 5% red onion peel+flesh (RPF). The results demonstrated that serum SOD activity was significantly increased in the RP and RPF groups, whereas glutathione peroxidase (GPx) activity was significantly higher in the RF group than in the ND group. Catalase activity and ORAC activity in liver showed upward tendency in the RP, RF, and RPF groups although the differences were not statistically significant. Liver malondialdehyde levels in the RPF group were significantly lower than those in the ND group were. In conclusion, red onion may enhance antioxidant defense mechanism through the induction of plasma SOD and GPx activities and inhibited liver lipid peroxidation. Therefore, red onion may exert important protective effects against oxidative stress related diseases. Crown Copyright © 2012. Published by Elsevier Ltd. All rights reserved.

  20. Antimicrobial and antioxidant activity of natural honeys of different origin

    Directory of Open Access Journals (Sweden)

    Miartina Fikselová

    2014-10-01

    Full Text Available To examine the antimicrobial and antioxidant activity of 15 natural honeys, honey samples were collected from different locations of Slovakia, Poland and Serbia. For antimicrobial activity determination honey solutions were prepared at three concentrations: 50, 25 and 12.5 % (by mass per volume. The potential antimicrobial activity of  selected samples against four species of bacteria (Escherichia coli CCM 3988, Pseudomonas aeroginosa CCM 1960, Staphylococcus epidermis CCM 4418, Bacillus cereus CCM 2010 and two species of yeasts (Saccharomyces cerevisiae CCM 8191, Candida albicans CCM 8216 was studied using the disc diffusion method. After incubation, the zones of inhibition of the growth of the microorganisms around the disks were measured. The strongest antimicrobial activity was shown at honey samples of 50 % concentration against Escherichia coli, Pseudomonas aeroginosa and Staphylococcus epidermis. Against Saccharomyces cerevisae and Candida albicans very low (at 50 %, 25 % concentration or zero antifugal (at 12.5 % concentration activity was determined. From the results obtained it was shown the variable ability of honey samples to scavenge stable free radical DPPH. TEACDPPH values ranged between 0.1-1.0 mmol.kg-1. As the antioxidative best source buckwheat honey was manifested and the lowest antioxidant activity was shown at acacia honey.

  1. Chemical composition and antioxidant activity of Origanum vulgare subsp. vulgare essential oil from Iran

    Directory of Open Access Journals (Sweden)

    M. Vazirian

    2015-12-01

    Full Text Available Background and Objectives: Essential oils are very complex mixture of components and their composition may vary in different species or varieties or even within the same variety. Origanum vulgare L. subsp. vulgare is one of the most distributed subspecies within the genus Origanum and has been found to be a poor-oil, categorized in cymyl, bornane or sabinyl chemotypes with higher proportion of sesquiterpenes. In this experiment, the Iranian sample was studied for the chemical composition of the oil and evaluation of its antioxidant activity. Methods: Essential oil was obtained by hydro-distillation and analyzed by GC/MS for determination of components. Antioxidant activity was evaluated by radical scavenging ability (DPPH method and reducing power (FRAP assay. Results: The sample belonged to “thymol” chemotype with the main components as thymol (37.13%, gama-terpinene (9.67%, carvacrol (9.57%, carvacrol methyl ether (6.88, cis-alpha-bisabolene (6.80%, eucalyptol (3.82%, p-cymene (3.58% and elemol (2.04%. The oil of plant showed very strong antioxidant activity (IC50=2.5 µg/mL in DPPH method, which was stronger than the standard antioxidants (Vit E and BHA, p

  2. Antioxidant and anti-tumor activity of a polysaccharide from freshwater clam, Corbicula fluminea.

    Science.gov (United States)

    Liao, Ningbo; Chen, Shiguo; Ye, Xingqian; Zhong, Jianjun; Wu, Nian; Dong, Shilei; Yang, Bo; Liu, Donghong

    2013-04-25

    The fresh water clam Corbicula fluminea is currently one of the most economically important aquatic species in China because of its nutritional value and pharmacological activity. In order to explore the potential of C. fluminea as a natural resource of bioactive compounds, a papain-released polysaccharide designated CFPS-2 was isolated. Chemical composition analysis indicated that CFPS-2 contained glucosamine, glucose, galactose, fucose, protein and sulfate groups, with an average molecular weight of about 22 kDa. Furthermore, the antioxidant and antitumor activities, in vitro, of the polysaccharide fractions (crude CFPS and purified CFPS-2) were evaluated. CFPS-2, which exhibited strong antioxidant activities in a dose dependent manner also showed significant inhibitory effects on growth of human gastric cancer cells (SGC7901) and human ovarian carcinoma cells (SKOV3 and A2780). The present results suggest that CFPS-2 could be a potential candidate for the development of novel functional food ingredient.

  3. Antioxidant activity of wild edible plants in the Black Sea Region of Turkey

    Energy Technology Data Exchange (ETDEWEB)

    Ozen, T.

    2010-07-01

    The anti oxidative activity of the 80% ethanol extract obtained from eleven commonly consumed wild edible plants was determined according to the phospho molybdenum method, reducing power, metal chelating, superoxide anion and free radical scavenging activity and compared to standard compounds such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and trolox. Total phenolic compounds, flavonoids, and anthocyanins in the extracts were also measured. Trachystemon orientalis, Vaccinium mrytillus, Rumex acetosella Polygonum amphibium, Beta vulgaris, and Similax Excelsa had the highest antioxidant capacities. Overall results showed that these plants can serve as good sources of bioactive polyphenols in the human diet and can be regarded as good candidates for nutritional supplement formulations due to their high concentrations of total phenolic compounds, flavonoids and anthocyanins as well as their strong antioxidant activity. (Author) 42 refs.

  4. Antioxidant Activity of Coconut (Cocos nucifera L.) Protein Fractions.

    Science.gov (United States)

    Li, Yan; Zheng, Yajun; Zhang, Yufeng; Xu, Jianguo; Gao, Gang

    2018-03-20

    Coconut cake is an abundant and good potential edible protein source. However, until now it has not been extensively used in the food industry. To promote its usage, the characterization, nutrition value and antioxidant activity of coconut cake protein fractions (albumin, globulin, prolamine, glutelin-1 and glutelin-2) were studied. Results revealed that all the albumin, globulin, glutelin-1 and glutelin-2 fractions showed a high nutrition value. The prolamine, glutelin-1 and glutelin-2 all exhibited good radical scavenging activity and reducing power, and the globulin and prolamine showed high ion chelating ability (89.14-80.38%). Moreover, all the fractions except glutelin-2 could effectively protect DNA against oxidative damage. Several peptides containing five to eight amino acids with antioxidant activity were also identified by LC-MS/MS from the globulin and glutelin-2 fractions. The results demonstrated that the coconut cake protein fractions have potential usages in functional foods.

  5. Orange Peel Extracts: Chemical Characterization, Antioxidant, Antioxidative Burst, and Phytotoxic Activities.

    Science.gov (United States)

    Erukainure, Ochuko L; Ebuehi, Osaretin A T; Iqbal Chaudhary, M; Mesaik, M Ahmed; Shukralla, Ahmed; Muhammad, Aliyu; Zaruwa, Moses Z; Elemo, Gloria N

    2016-01-01

    The search for novel drugs and alternative medicine has led to increased research in medicinal plants. Among such plants is the orange fruit. Its peels have been utilized for long as an active ingredient in most traditional medicines. This study aims at investigating the chemical properties of the hexane and dichloromethane (DCM) extracts of orange peel as well as their biological potentials. Blended peels were extracted with n-hexane and n-dichloromethane, respectively. The resulting extracts were subjected to gas chromatography mass spectrometry (GCMS) characterization. The extracts were also assayed for free radical scavenging ability against 1,1 -diphenyl -2 picrylhydrazyl (DPPH), antioxidative burst via measuring luminol -amplified chemiluminescence response in human blood, and phytotoxicity against lemna minor. GCMS analysis revealed a predominance of fatty acid methyl esters in the hexane extract, while the DCM extract had more ketone metabolites. The DCM extract had significant (p < .05) higher free radical scavenging and antioxidative burst activities compared to the hexane. Both extracts revealed a significantly (p < .05) high phytotoxicity activity. Results from this study indicated that solvent type played a vital a role in the extraction of secondary metabolites, which are responsible for the observed biological activities. The higher activities by the DCM extract can be attributed to its constituents as revealed by GCMS analysis. There is great need to explore the phytotoxicity potentials of both extracts as natural herbicides.

  6. Antioxidant and antibacterial activity of Thai medicinal plant (Capparis micracantha)

    Science.gov (United States)

    Laoprom, Nonglak; Sangprom, Araya; Chaisri, Patcharaporn

    2018-04-01

    This work aims to study the antioxidants capacity, Total phenolic content and antibacterial activity of Thai medicinal plant for the treatment of dermatitis-related inflammations, Capparis micracantha. Crude extract from stem of Thai medicinal plant was extracted with hexane, ethyl acetate, methanol and water. The antioxidant activities (IC50) was evaluated with 1,1-diphenyl-1-princylhydrazyl (DPPH) radical scavenging assay. Total phenolic content (TPC) was determined by using Folin-Ciocalteu method. Bacterial activities was tested with four human pathogenic bacteria; Escherichia coli, Listeria monocytogenes, Staphylococcus aureus and Stapylococcus epidermidis by using agar diffusion assay. Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were also determined by broth dilution method. For antioxidant activity, the methanol fraction from stem extract showed the highest activity with an IC50 of 2.4 mg/ml. Water extraction was the high TPC with 10,136.9 mg GAE/g dry weight. Methanol and water extraction showed the remarkable inhibition of bacterial growth was shown against L. monocytogenes and S. aureus. In addition, ethyl acetate, methanol and water fraction from stem extract against S. epidermidis. The present finding suggests that the extract of C. micracantha could be used to discover bioactive natural products that may serve as pharmaceutical products.

  7. Antioxidant and cyclooxygenase activities of fatty acids found in food.

    Science.gov (United States)

    Henry, Geneive E; Momin, Rafikali A; Nair, Muraleedharan G; Dewitt, David L

    2002-04-10

    Several commercially available C-8 to C-24 saturated and unsaturated fatty acids (1-29) were assayed for cyclooxygenase-I (COX-I) and cyclooxygenase-II (COX-II) inhibitory and antioxidant activities. Among the saturated fatty acids tested at 60 microg mL(-1), there was an increase in antioxidant activity with increasing chain length from octanoic acid to myristic acid (C-8-C-14) and a decrease thereafter. All unsaturated fatty acids tested at 60 microg mL(-1) showed good antioxidant activity except for undecylenic acid (12), cis-5-dodecenoic acid (13), and nervonic acid (29). The highest inhibitory activities among the saturated fatty acids tested on cyclooxygenase enzymes COX-I and COX-II were observed for decanoic acid to lauric acid (3-5) at 100 microg mL(-1). Similarly, among the unsaturated fatty acids tested, the highest activities were observed for cis-8,11,14-eicosatrienoic acid (25) and cis-13,16-docosadienoic acid (27) at 100 microg mL(-1).

  8. Chemical composition and antioxidant activity of two strawberry cultivars

    Directory of Open Access Journals (Sweden)

    Đilas Sonja M.

    2011-01-01

    Full Text Available The macro- and micro-chemical composition, as well as antioxidant activity of two strawberry cultivars, Marmolada and Clery, were studied. Results showed a noticeable difference in the sugar, protein and pectin contents. Clery had 6.92% and Marmolada 4.93% of total sugar. Also, protein and pectin contents were higher in the Clery cultivar. No significant difference was observed in acidity, as well as in ash and cellulose content. Marmolada had a higher content of total phenolics and flavonoids (228.04 mg GAE /100 g FW and 136.01 mg RE/100 g FW, respectively . The anthocyanins content in Marmolada (32.0 mg CGE/100 g FW was slightly lower than in Clery (36.0 mg CGE/100 g FW. The antioxidant activity was evaluated spectrophotometrically, using 2,2-diphenyl- 1-picrylhydrazyl (DPPH radical scavenging activity assay. The DPPH free radical scavenging activity, expressed as EC50 value, of Marmolada (0.77 mg/ml was higher than of Clery (0.83 mg/ml. There was a significant positive correlation (R2>0.90 between the concentration of phenolics/flavonoids/anthocyanins and DPPH radical scavenging activity of both strawberry cultivars. These results also showed that the antioxidant value of 100 g FW Marmolada and Clery is equivalent to 237.91 mg and 219.01 mg of vitamin C, respectively.

  9. Antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts

    Directory of Open Access Journals (Sweden)

    Chaabane Fadwa

    2012-09-01

    Full Text Available Abstract Background Plants play a significant role in maintaining human health and improving the quality of human life. They serve humans well as valuable components of food, as well as in cosmetics, dyes, and medicines. In fact, many plant extracts prepared from plants have been shown to exert biological activity in vitro and in vivo. The present study explored antioxidant and antigenotoxic effects of Daphne gnidium leaf extracts. Methods The genotoxic potential of petroleum ether, chloroform, ethyl acetate, methanol and total oligomer flavonoid (TOF enriched extracts from leaves of Daphne gnidium, was assessed using Escherichia coli PQ37. Likewise, the antigenotoxicity of the same extracts was tested using the “SOS chromotest test”. Antioxidant activities were studied using non enzymatic and enzymatic method: NBT/Riboflavine and xantine oxidase. Results None of the different extracts produced a genotoxic effect, except TOF extract at the lowest tested dose. Our results showed that D. gnidium leaf extracts possess an antigenotoxic effect against the nitrofurantoin a mutagen of reference. Ethyl acetate and TOF extracts were the most effective in inhibiting xanthine oxidase activity. While, methanol extract was the most potent superoxide scavenger when tested with the NBT/Riboflavine assay. Conclusions The present study has demonstrated that D. gnidium leaf extract possess antioxidant and antigenotoxic effects. These activities could be ascribed to compounds like polyphenols and flavonoid. Further studies are required to isolate the active molecules.

  10. Antioxidant and antimutagenic activity of Curcuma caesia Roxb. rhizome extracts

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    Heisanam Pushparani Devi

    2015-01-01

    Full Text Available The rhizomes of Curcuma caesia Roxb. (zingiberacea are traditionally used in treatment of various ailments and metabolic disorders like leukoderma, asthma, tumours, piles, bronchitis, etc. in Indian system of medicine. Considering the importance of natural products in modern phytomedicine, the antioxidant and antimutagenic activities of C. caesia Roxb. rhizome extract and its fractions were evaluated. The ethanolic fraction showed highest antioxidant activity by DPPH assay (86.91% comparable to ascorbic acid (94.77% with IC50 value of 418 μg/ml for EECC followed by MECC (441.90 μg/ml > EAECC(561 μg/ml > AECC(591 μg/ml. Based on the antioxidant activity, three of the rhizome extracts were evaluated for their antimutagenic properties against indirect acting mutagen cyclophosphamide (CP using Salmonella typhimurium strains TA98 and TA100. The antimutagenic activity of the extracts against indirect acting mutagen cyclophosphamide in the presence of mammalian metabolic activation system was found to be significant (p < 0.01, p < 0.05. All the extracts showed similar antimutagenicity in dose dependent manner. The total phenolic content as well as reducing ability of the extracts was also determined.

  11. Evaluation and comparison of the content of total polyphenols and antioxidant activity of selected species of the genus Allium

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    Marianna LENKOVÁ

    2016-12-01

    Full Text Available The species of the genus Allium are very important crops for human health. They contain many health beneficial substances, such as polyphenols (especially flavonoids, sulphur compounds, vitamins, mineral substances and substances with antioxidant activity. This work has focused on the comparison of total phenolic content and antioxidant activity of selected species of the genus Allium – garlic (Allium sativum L., chives (Allium schoenoprasum L., ramson (Allium ursinum L. and red, yellow and white onion (Allium cepa L.. Samples of plant material were collected at the stage of full maturity in the area of Nitra. Total polyphenols content was determined using the spectrophotometric method of Folin-Ciocalteu agents. Determined the content of total polyphenols were in the range 444.3 - 1591 mg*kg-1. Total polyphenols content in the observed crops declined in the following order: chives > red onion > garlic > yellow onion > ramson > white onion. Antioxidant activity was measured by the spectrophotometric method using a compound DPPH (2.2-diphenyl-1-picryhydrazyl. Determined the value of antioxidant activity ranged 12.29 – 76.57%. Antioxidant activity observed in crops declined in the following order: chives > ramson > red onion > yellow onion > garlic > white onion. In all the analysed crop plants was confirmed by the strong dependence of the antioxidant activity and the total content of polyphenolic substances.

  12. Evaluation of Antioxidant Activities of Ampelopsin and Its Protective Effect in Lipopolysaccharide-Induced Oxidative Stress Piglets

    Science.gov (United States)

    Hou, Xiang; Zhang, Jingfei; Ahmad, Hussain; Zhang, Hao; Xu, Ziwei; Wang, Tian

    2014-01-01

    The aim of this study was to investigate the antioxidant potential of ampelopsin (APS) by using various methods in vitro, as well as to determine effects of APS on LPS-induced oxidative stress in piglets. The results showed that APS exhibited excellent free radical scavenging by DPPH, ABTS, O2•−, H2O2 and ferric reducing antioxidant power. Ampelopsin also protected pig erythrocytes against AAPH-induced apoptosis and hemolysis, decreased total superoxide dismutase activity, and increased lipid peroxidation. Furthermore the results demonstrated that APS enhanced the total antioxidant capacity and decreased the malondialdehyde and protein carbonyl contents in LPS-treated piglets. The results of the present investigation suggest that APS possesses a strong antioxidant activity and alleviates LPS-induced oxidative stress, possibly due to its ability to prevent reactive oxygen species. PMID:25268121

  13. Development of Antioxidant Activity during Milk Fermentation by Wild Isolates of Lactobacillus helveticus

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    Aazam Namdari

    2016-07-01

    Full Text Available Background and Objective: Oxidative stress, due to free radicals, brings injury to the body by attacking large molecules and cell organs, and is the main reason of many diseases. Fermentation of foods containing large amount of proteins such as milk by special species of lactic acid bacteria is a potential way in enhancement of the antioxidative activity of foods. This study aimed at evaluating non-common starter species isolates of Lactobacillus helveticus for their capability to produce fermented milk enriched in antioxidant peptides.Materials and Methods: Reconstituted skim milk (11% was inoculated with 7 wild isolates of Lactobacillus helveticus, and after 24 h fermentation at 37ºC, the samples were kept 4ºC and for 14 days. Viable cell number, acidification and proteolysis degree in the milk fermented by each isolate were assessed in 1, 7 and 14 days. Development of antioxidant activity was measured using DPPH and ABTS●+ radial scavenging activities during the storage period.Results and Conclusion: Though some slight strain-dependent differences were observed in growth, acidification and proteolysis, all the samples showed considerably strong antioxidant activity (at least 62.32±3.66% and 57.64±1.42% measured using DPPH and ABTS●+ radicals, respectively through the whole storage period. In vitro simulated gastrointestinal digestion indicated that DPPH radical-scavenging activity of the antioxidative peptidic supernatants was not affected significantly by consecutive pepsin-pancreatin hydrolysis in most of the samples. These evidences support Lactobacillus helveticus as a promising functional culture able to promote health benefits in dairy-based functional foods.Conflict of interest: The authors declare that there is no conflict of interest.

  14. Antioxidative, anticholinesterase and antityrosinase activities of the ...

    African Journals Online (AJOL)

    user

    2012-05-15

    May 15, 2012 ... In addition to undesirable color and flavor, the quinine compounds produced in the browning ... necessary to search and develop novel tyrosinase inhibitors with potent activities and fewer side effects. Dementia is a chronic progressive mental disorder, which adversely affects memory, thinking, compre-.

  15. Bioactive content, hepatoprotective and antioxidant activities of ...

    African Journals Online (AJOL)

    2Department of Pharmacology and Toxicology, Dubai Pharmacy College, Dubai, United Arab Emirate, 3Department of. Pharmaceutical ... biological activities. Methods: Reversed-phase high-performance liquid chromatography (RP-HPLC) was employed for the ..... Yet, clinical application of those plants needs extensive in ...

  16. Fermentation optimization and antioxidant activities of mycelia ...

    African Journals Online (AJOL)

    The mycelia polysaccharides from Morchella esculenta are active ingredients in a number of medicines that play important roles in immunity improvement and tumor growth inhibition. So far, the production of polysaccharides from M. esculenta mycelia has not been commercialized. The aims of this work were to screen and ...

  17. Carotenoids from Phaffia rhodozyma: Antioxidant activity and ...

    African Journals Online (AJOL)

    Aghomotsegin

    2015-06-10

    Jun 10, 2015 ... billion in 2018. They are mostly produced by chemical synthesis. However, the biotechnological production of these pigments by yeasts has been highlighted for possibly using low cost substrates in cultivation, control of metabolites with biological activity, designation of natural substances, the small space ...

  18. Antioxidative, anticholinesterase and antityrosinase activities of the ...

    African Journals Online (AJOL)

    user

    2012-05-15

    May 15, 2012 ... 1Department of Marine Food Science and Technology, Gangeung-Wonju National University, Gangneung 210-702,. Republic of ... The solvent extracts of a red alga Grateloupia lancifolia were used to evaluate biofunctional activities. ...... content, and peroxide value of essential oil and extracts of some.

  19. Antimicrobial, antioxidant and anticancer activities of Strychnos ...

    African Journals Online (AJOL)

    Background: Strychnos lucida R. Br. (Loganiaceae), a well-known indigenous medicine in Timor Leste, has been used for the treatment of ailments such as malaria, diarrhoea, fever, hypertension, cancer, diabetes mellitus and skin infections. Its pharmacological activity has never been reported. The aim of this study was to ...

  20. Antioxidant potentialities and Antiradical Activities of Oxalis ...

    African Journals Online (AJOL)

    SARAH

    2017-08-31

    Aug 31, 2017 ... Eaten raw or added to stew to make it taste, juice taken by sick people to ... Food, medicinal Eaten as raw vegetable, its juice used as medicine to treat, wound, stomach upset, flue in infants and spice to make the stew tasty. 12. Ha. NK. Food and ..... biological activities. For example, Cushine and Lamb.

  1. Hepatoprotective and Antioxidant Activities of Tribulus Terrestris

    NARCIS (Netherlands)

    Harraz, Fathalla M; Ghazy, Nabila M; Hammoda, Hala M; Nafeaa, Abeer A.; Abdallah, Ingy I.

    2015-01-01

    Tribulus terrestris L. has been used in folk medicine throughout history. The present study examined the acute toxicity of the total ethanolic extract of T. Terrestris followed by investigation of the hepatoprotective activity of the total ethanolic extract and different fractions of the aerial

  2. Polyphenols content and antioxidant activity of paprika and pepper spices

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    Soňa Škrovánková

    2017-01-01

    Full Text Available Paprika spices (Capsicium annuum and black pepper spices (Piper nigrum are very popular seasonings for culinary and industrial utilization due to the change of sensory quality (taste, aroma, color of foods and meals with their addition; their health promoting properties; and also, relevant antioxidant activity. Polyphenols are often responsible for the antioxidant capacity of plant products therefore in our study the content of polyphenols (TP and antioxidant activity (TAA were assessed in two common culinary spices - paprika spices (12, ground powder spices and pepper spices (20, unground and ground, black, green, white and colored spices of Czech, Austrian, and Slovak producers. These parameters were determined using spectrometric method, for total polyphenols method with Folin-Ciocaulteu reagent; the antioxidant activity (TAA of aqueous and ethanolic extracts of spices was measured by DPPH method with IC50 evaluation. For paprika the total polyphenol content ranged from 14.67 to 28.78 mg GAE.g-1. However, there is only weak connection between the pungency of the spices and the polyphenolic amount, the hotter samples of paprika spices have slightly higher values of TP than sweet types. Also, more pungent paprika products showed a higher potency in scavenging of DPPH free radical than sweeter ones; and ethanolic extracts had slightly higher TAA values (8.73 to 16.17 mg AAE.g-1 than aqueous spice extracts (4.45 to 16.24 mg AAE.g-1. Phenolic amount for pepper spices was assessed in the range of 12.03 to 22.88 mg GAE.g-1. Generally, paprika spices contained more polyphenols than pepper spices. The values of TAA of pepper spices were in the range from 7.07 to 15.81 mg AAE.g-1 for aqueous extracts and from 8.25 to 15.93 mg AAE.g-1 for ethanolic extracts respectively. The highest TAA values were observed for white ground pepper and unground black pepper spices. Unground black pepper samples had higher TAA than ground black pepper. The extent of

  3. Evaluation of the antioxidant and hepatoprotective activity of Terminalia catappa.

    Science.gov (United States)

    Lin, C C; Chen, Y L; Lin, J M; Ujiie, T

    1997-01-01

    The free radical scavenging and antihepatotoxic activity from Terminalia catappa was studied. Treatment with T. catappa water extracts showed antihepatotoxic activity against CCl4-induced toxicity in the rat liver that was tested. The crude drug also exhibited anti-oxidant effects in FeCl2-Ascorbic acid induced lipid peroxidation in the rat liver homogenate. Moreover, the superoxide radical scavenger effect of T. catappa was demonstrated using electron spin resonance (ESR) and spintrapping technique. The results indicate that T. catappa possesses good antihepatotoxic activity and superoxide radical scavenger activity.

  4. Standardization and in vitro antioxidant activity of jatamansi rhizome

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    Mhaveer Singh

    2015-01-01

    Full Text Available Background: Nardostachys jatamansi Linn. commonly known as jatamansi is a well notorious drug in Indian systems of medicines having various health-related benefits and employed in various herbal formulations due to the presence of high levels of valuable phenolic constituents. The present study was aimed to quality assessment of Jatamansi rhizome by studying macro- and micro-scopic characters along with physicochemical tests, chemo-profiling using thin layer chromatography (TLC, and gas chromatography–mass spectrometry (GC-MS, in vitro antioxidant activity. Materials and Methods: Standardization was carried out as per the pharmacopeial guidelines and contaminant estimation was carried out by analyzing the samples for the determination of heavy metals, pesticides, and aflatoxins. Chemo-profiling was done with TLC by optimizing the mobile phase for different extracts. The GC-MS chemo-profiling was also carried out by using hexane soluble fraction of the hydroalcoholic extract. The drug is well known for a protective role in the human body as an antioxidant, so total phenolic contents and in vitro antioxidant efficacy was also determined by using established methods. Results:The results of quality control and anatomical studies were very much useful for its identification, whereas significant antioxidant efficacy was also observed. The drug was found free of contaminants when analyzed for pesticides and aflatoxins, whereas heavy metals were found under the pharmacopeial limit. Conclusion: The findings of the present research can be utilized for the identification and quality control of the jatamansi rhizome.

  5. Determination of antimicrobial effect, antioxidant activity and ...

    African Journals Online (AJOL)

    In addition, the phenolic contents were determined as follows: the catechin was 20 mg/g, the ferulic acid was 15 mg/g, the p-coumaric acid was 10 mg/g, and the cinnamic acid was 6 mg/g. The results showed that T. boudieri has antimicrobial activity on the gram negative and positive bacteria as well as yeast, and it also has ...

  6. Chemical composition and antioxidant activity of berry fruits

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    Stajčić Slađana M.

    2012-01-01

    Full Text Available The main chemical composition, contents of total phenolic (TPh, total flavonoid (TF, and total monomeric anthocyianin (TMA, as well as the antioxidant activity of two raspberry cultivars (Meeker and Willamette, two blackberry cultivars (Čačanska bestrna and Thornfree and wild bilberry were studied. The raspberry cultivars had the highest total solids among fruits investigated. Bilberry fruits had the highest sugar-to-acid ratio. Blackberry fruits were richer in crude fibers (cellulose in comparison to raspberry and bilberry fruits. The content of pectic substances was highest in the bilberry. Also, bilberry had a highest content of TPh (808.12 mg GAE/100 g FW, TF (716.31 mg RE/100 g FW and TMA (447.83 mg CGE/100 g FW. The antioxidant activity was evaluated spectrophotometrically, using 2,2-diphenyl-1-picrylhydrazyl (DPPH radical scavenging activity assay. The DPPH free radical scavenging activity, expressed as the EC50 value (in mg of fresh weight of berry fruit per ml of the reaction mixture, of bilberry (0.3157 ± 0.0145 mg/ml was the highest. These results also showed that the antioxidant value of 100 g FW bilberry, raspberry - Willamette, raspberry - Meeker, blackberry - Čačanska bestrna and blackberry - Thornfree is equivalent to 576.50 mg, 282.74 mg, 191.58 mg, 222.28 mg and 272.01 mg of vitamin C, respectively. There was a significant positive correlation between the antioxidant activities and content of total phenolics (RTPh 2=0.9627, flavonoids (RTF 2=0.9598 and anthocyanins (RTMA 2=0.9496 in berry fruits. [Projekat Ministarstva nauke Republike Srbije, br. TR 31044

  7. Antioxidant activity and optimization of extraction of polysaccharide from the roots of Dipsacus asperoides.

    Science.gov (United States)

    Tan, Li-Hong; Zhang, Dan; Yu, Bao; Zhao, Sheng-Ping; Wang, Jian-Wei; Yao, Ling; Cao, Wei-Guo

    2015-11-01

    Polysaccharide extraction from Dipsacus asperoides roots (DAP) was proved to possess strong antioxidant activities, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-Azobis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging activities, inhibiting β-carotene bleaching and strong reducing power. Cell assay demonstrated that the crude DAP possessed antioxidant activity and were effective against H2O2-induced L02 cells injury. Then, response surface methodology (RSM) was applied to optimize the ultrasonic extraction of DAP. The optimum variables given by central composite design (CCD) were as follows: ratio of water to raw material, 38.61mL/g; ultrasonic power, 308.68W; extraction time, 38.61min; and extraction temperature, 89°C. Under these conditions, the maximum yield of DAP obtained was 7.12±0.45%. Moreover, high performance liquid chromatography (HPLC) analysis suggested that the monosaccharide compositions of DAP contained primarily mannose, ribose, glucose, galactose, xylose and arabinose, with a molar ratio of 0.22:0.48:2.29:0.34:1.39:1.41. The results of the present study showed that DAP could be considered as potential sources of natural antioxidants. Copyright © 2015 Elsevier B.V. All rights reserved.

  8. Total phenolics and antioxidant activity of five medicinal plant

    International Nuclear Information System (INIS)

    Sousa, Cleyton Marcos de M.; Silva, Hilris Rocha e; Vieira-Junior, Gerardo Magela; Ayres, Mariane Cruz C.; Costa, Charllyton Luis S. da; Araajo, Delton Servulo; Cavalcante, Luis Carlos D.; Barros, Elcio Daniel S.; Araujo, Paulo Breitner de M.; Brandao, Marcela S.; Chaves, Mariana H.

    2007-01-01

    This paper describes total phenolics content and antioxidant activity in the ethanolic extract of leaves, bark and roots of five medicinal plants: Terminalia brasiliensis Camb., Terminalia fagifolia Mart. and Zucc., Copernicia cerifera (Miller) H.E. Moore, Cenostigma macrophyllum Tul. var. acuminata Teles Freire and Qualea grandiflora Mart. The total phenolics content of the plant extracts, determined by the Folin-Ciocalteu method, varied from 250.0 ±8,2 to 763,63 ±13.03 mg of gallic acid equivalent/g dry EtOH extract. The antioxidant activity of extracts was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay system. Extract of bark from T. brasiliensis, the most active, with an EC 50 value of 27.59 ± 0.82 μg/mL, was comparable to rutin (EC 50 = 27.80 ± 1.38) and gallic acid (EC 50 = 24.27 ± 0.31), used as positive controls. The relationship between total phenolic content and antioxidant activity was positive and significant for T. brasiliensis, C. macrophyllum and C. cerifera. (author)

  9. Rosmarinus officinalis essential oil: antiproliferative, antioxidant and antibacterial activities

    Science.gov (United States)

    Hussain, Abdullah Ijaz; Anwar, Farooq; Chatha, Shahzad Ali Shahid; Jabbar, Abdul; Mahboob, Shahid; Nigam, Poonam Singh

    2010-01-01

    The aim of this work was to investigate and compare the antiproliferative, antioxidant and antibacterial activities of Rosmarinus officinalis essential oil, native to Pakistan. The essential oil content from the leaves of R. officinalis was 0.93 g 100g-1. The GC and GC-MS analysis revealed that the major components determined in R. officinalis essential oil were 1,8-cineol (38.5%), camphor (17.1%), α-pinene (12.3%), limonene (6.23%), camphene (6.00%) and linalool (5.70%). The antiproliferative activity was tested against two cancer (MCF-7 and LNCaP) and one fibroblast cell line (NIH-3T3) using the MTT assay, while, the antioxidant activity was evaluated by the reduction of 2, 2-diphenyl-1-picryl hydrazyl (DPPH) and measuring percent inhibition of peroxidation in linoleic acid system. The disc diffusion and modified resazurin microtitre-plate assays were used to evaluate the inhibition zones (IZ) and minimum inhibitory concentration (MIC) of R. officinalis essential oil, respectively. It is concluded from the results that Rosmarinus officinalis essential oil exhibited antiproliferative, antioxidant and antibacterial activities. PMID:24031588

  10. Antioxidant and Antimicrobial Activities of Pogostemon cablin (Blanco Benth.

    Directory of Open Access Journals (Sweden)

    Bhanuz Dechayont

    2017-01-01

    Full Text Available Pogostemon cablin (Lamiaceae has been widely used in traditional medicine. In this study, the antioxidant and antimicrobial activities of leaves from P. cablin extracts were investigated. The water extracts had the highest total phenolic content 116.88±0.48 mg gallic acid equivalent/g of dry plant extract. Nevertheless, high levels of total flavonoid content were found in ethanolic extracts 280.12±2.04 mg quercetin equivalent/g of dry plant extract. The highest antioxidant activities were found for the ethanolic extract (IC50=18±0.90, 20±0.24 μg/mL by DPPH and ABTS scavenging assays, respectively. Both extracts showed moderate inhibition of superoxide inhibition (O2∙- and nitric oxide (NO production in concentration-dependent manner. Antibacterial activity was calculated by disk diffusion, minimum inhibitory concentration (MIC, and minimum bacterial concentration (MBC. The ethanolic extract had the greatest activity against methicillin resistant Staphylococcus aureus, methicillin sensitive S. aureus, and Streptococcus pyogenes with zone diameters of 11.67±1.53, 10.33±2.52, and 10.33±1.15 mm, respectively. The corresponding MIC and MBCs were 5, 0.625, and 0.039 mg/mL. P. cablin extracts contain antioxidant and antibacterial properties that should be exploited for possible clinical application.

  11. Rosmarinus officinalis essential oil: antiproliferative, antioxidant and antibacterial activities

    Directory of Open Access Journals (Sweden)

    Abdullah Ijaz Hussain

    2010-12-01

    Full Text Available The aim of this work was to investigate and compare the antiproliferative, antioxidant and antibacterial activities of Rosmarinus officinalis essential oil, native to Pakistan. The essential oil content from the leaves of R. officinalis was 0.93 g 100g-1. The GC and GC-MS analysis revealed that the major components determined in R. officinalis essential oil were 1,8-cineol (38.5%, camphor (17.1%, α-pinene (12.3%, limonene (6.23%, camphene (6.00% and linalool (5.70%. The antiproliferative activity was tested against two cancer (MCF-7 and LNCaP and one fibroblast cell line (NIH-3T3 using the MTT assay, while, the antioxidant activity was evaluated by the reduction of 2, 2-diphenyl-1-picryl hydrazyl (DPPH and measuring percent inhibition of peroxidation in linoleic acid system. The disc diffusion and modified resazurin microtitre-plate assays were used to evaluate the inhibition zones (IZ and minimum inhibitory concentration (MIC of R. officinalis essential oil, respectively. It is concluded from the results that Rosmarinus officinalis essential oil exhibited antiproliferative, antioxidant and antibacterial activities.

  12. Rosmarinus officinalis essential oil: antiproliferative, antioxidant and antibacterial activities.

    Science.gov (United States)

    Hussain, Abdullah Ijaz; Anwar, Farooq; Chatha, Shahzad Ali Shahid; Jabbar, Abdul; Mahboob, Shahid; Nigam, Poonam Singh

    2010-10-01

    The aim of this work was to investigate and compare the antiproliferative, antioxidant and antibacterial activities of Rosmarinus officinalis essential oil, native to Pakistan. The essential oil content from the leaves of R. officinalis was 0.93 g 100g(-1). The GC and GC-MS analysis revealed that the major components determined in R. officinalis essential oil were 1,8-cineol (38.5%), camphor (17.1%), α-pinene (12.3%), limonene (6.23%), camphene (6.00%) and linalool (5.70%). The antiproliferative activity was tested against two cancer (MCF-7 and LNCaP) and one fibroblast cell line (NIH-3T3) using the MTT assay, while, the antioxidant activity was evaluated by the reduction of 2, 2-diphenyl-1-picryl hydrazyl (DPPH) and measuring percent inhibition of peroxidation in linoleic acid system. The disc diffusion and modified resazurin microtitre-plate assays were used to evaluate the inhibition zones (IZ) and minimum inhibitory concentration (MIC) of R. officinalis essential oil, respectively. It is concluded from the results that Rosmarinus officinalis essential oil exhibited antiproliferative, antioxidant and antibacterial activities.

  13. Phytochemicals Content, Antioxidant and α-Glucosidase Inhibition Activity of Bouea Macrophylla Griff Seed Extract

    International Nuclear Information System (INIS)

    Zainah Adam; Hazlina Ahmad Hassali; Rosniza Razali

    2016-01-01

    Bouea macrophylla Griff or locally known as kundang is one of the common fruit plant available in Malaysia. This plant from Anacardiaceae family is native to Southeast Asia particularly in Malaysia, Thailand and Indonesia. Medicinal values of this plant is not yet been explored. The present study was done to evaluate phytochemicals constituents in B. macrophylla seed extract qualitatively and quantitatively. Biological evaluations focusing on antioxidant and α-glucosidase inhibition were also performed. Qualitative phytochemicals screening revealed the presence of anthraquinones, terpenoids, flavanoids, tannins, alkaloids, glycosides, reducing sugar, steroids, triterpenes, phenolic, coumarine and proteins in B. macrophylla seed extract. Quantitative determination showed that B. macrophylla seed extract contains high amount of phenolic compounds (689.17±37.50 mg GAE/ g extract), but low amount of flavonoids (2.78±0.01 mg QE/ g extract), suggesting that most of the phenolics in B. macrophylla seed extract were non-flavonoids. Antioxidant assays showed that the extract possesses strong reducing power and DPPH radical scavenging activity (IC 50 : 4.73±0.51 μg/ ml). These activities were almost comparable to that of vitamin C. α-Glucosidase inhibition study showed that the extract inhibited alpha-glucosidase activity potently with the IC 50 value of 0.55±0.04 mg/ ml, suggesting the ability of the plant to delay glucose absorption in small intestine, hence reduces hyperglycemia in diabetic condition. Potent antioxidant and α-glucosidase inhibitory activity of the extract might be attributed to the presence of high amount of phenolic compounds. In conclusion, this study showed that B. macrophylla seed extract contains various phytochemicals, possess strong antioxidant property and showed promising antidiabetic activity. These results indicate that B. macrophylla might have the potential to be developed as new pharmacological agent targeting on oxidative stress

  14. Sulfated polysaccharides with antioxidant and anticoagulant activity from the sea cucumber Holothuria fuscogliva

    Science.gov (United States)

    Li, Rongfeng; Yu, Huahua; Yue, Yang; Liu, Song; Xing, Rong'e.; Chen, Xiaolin; Li, Pengcheng

    2017-07-01

    Sea cucumber is a traditional nutritional food and medicinal resource with many bioactive components in China. Holothuria fuscogliva is a big sea cucumber with a rich of bioactive polysaccharides. To investigate the bioactivities of the polysaccharides from sea cucumber H. fuscogliva, we prepared the sulfated polysaccharides (HfP) from sea cucumber H. fuscogliva using a protease hydrolysis method. Antioxidant activities of HfP were investigated, including hydroxyl radical scavenging activity and superoxide radical scavenging activity. And, the anticoagulant activities of HfP were studied, including the activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT). The average molecular weight was 1 867.1 Da, with a sulfate content of 20.7%. In addition, the molar ratio of monosaccharide composition of HfP was Man: Rha: Glc A: Glc: Gal: Xyl: Fuc=0.083 6: 0.437: 0.134: 0:1.182: 0.748: 1. It had a strong antioxidant activity, the hydroxyl and superoxide radical scavenging activity EC50 of HfP was 3.74 and 0.037 mg/mL, respectively. It also showed a good anticoagulant activity in our study. The APTT of HfP was much higher than that of heparin sodium, and the PT and TT of HfP was close to that of heparin sodium at a low concentration. Therefore, HfP shows a good antioxidant and anticoagulant activity and it may become a potential candidate of the natural antioxidant and anticoagulant and will have a good application future in health product or medicine industry.

  15. Antimutagenic and antioxidant activities of some bioflavours from wine.

    Science.gov (United States)

    Antonella, Di Sotto; Federico, Durazzi; Grazia, Sarpietro Maria; Gabriela, Mazzanti

    2013-10-01

    Monoterpenes limonene and its metabolic derivatives, α-terpineol and 1,8-cineol, commonly found as aroma wine components, were studied for their antimutagenicity by the bacterial reverse mutation assay on different strains. Substances were also tested for their antioxidant activity, i.e. radical scavenger, chelation, reduction, and lipid peroxidation inhibition. Limonene and its metabolites, α-terpineol and 1,8-cineol, resulted able to inhibit the chemically-induced mutagenesis, although with a different specificity. The antimutagenicity of limonene has been generally retained by its metabolites and sometimes increased. In particular, α-terpineol exhibited the strongest inhibition, moreover it showed to be a remarkable ferrous ions chelating agent. Limonene and 1,8-cineol were devoid of antioxidant activity. Present results are a starting point in evaluating the potential of α-terpineol as a chemopreventive agent and suggest potential functional dietary benefits of wine. Copyright © 2013 Elsevier Ltd. All rights reserved.

  16. Tannins from Canarium album with potent antioxidant activity*

    Science.gov (United States)

    Zhang, Liang-liang; Lin, Yi-ming

    2008-01-01

    The contents of total phenolics and extractable condensed tannins in the leaves, twigs and stem bark of Canarium album were determined. The structural heterogeneity of condensed tannins from stem bark was characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nuclear magnetic resonance (NMR) analyses. The results show the predominance of signals representative of procyanidins and prodelphinidins. In addition, epicatechin and epigallocatechin polymers with galloylated procyanidin or prodelphinidin were also observed. The tannins were screened for their potential antioxidant activities using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) model systems. Tannins extracted from leaves, twigs and stem bark all showed a very good DPPH radical scavenging activity and ferric reducing power. PMID:18500781

  17. Tannins from Canarium album with potent antioxidant activity.

    Science.gov (United States)

    Zhang, Liang-liang; Lin, Yi-ming

    2008-05-01

    The contents of total phenolics and extractable condensed tannins in the leaves, twigs and stem bark of Canarium album were determined. The structural heterogeneity of condensed tannins from stem bark was characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nuclear magnetic resonance (NMR) analyses. The results show the predominance of signals representative of procyanidins and prodelphinidins. In addition, epicatechin and epigallocatechin polymers with galloylated procyanidin or prodelphinidin were also observed. The tannins were screened for their potential antioxidant activities using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) model systems. Tannins extracted from leaves, twigs and stem bark all showed a very good DPPH radical scavenging activity and ferric reducing power.

  18. In vitro cytotoxic and antioxidant activities of phenolic components of Algerian Achillea odorata leaves

    Directory of Open Access Journals (Sweden)

    Hanane Boutennoun

    2017-03-01

    Full Text Available In this study, methanol extract from Achillea odorata was evaluated for its phenolic contents using Folin–Ciocalteu reagent, and antioxidant activity using: 1,1-diphenyl-2-picrylhidrazyl (DPPH radical scavenging activity, reducing activity of H2O2 and ferric reducing power assay. The total phenolic content was determined as gallic acid (GAE equivalent. Flavonoids and flavonols contents were determined as quercetin (QE equivalents. The cytotoxicity of the plant extract was tested against three tumor cell lines: MCF-7, Hep2 and WEHI using 3-(4,5-dimethyl thiazol-2-yl-2,5-diphynyl tetrazolium bromide (MTT assay. Preliminary screening was concluded in the presence of substances with large therapeutic values. The total phenolic content confirmed the presence of total phenolics in the extract and showed strong association with antioxidant activity. An important content of flavonoids and flavonols was also detected. The results of the antioxidant activities obtained indicate that A. odorata recorded a good capacity. For the cytotoxic activity, the results showed the plant extract significantly inhibited tumor cell growth and colony formation at various concentrations.

  19. Evaluation of the antioxidant activity of rice bran extracts using different antioxidant assays

    Directory of Open Access Journals (Sweden)

    Rehman Bajwa, Jawad -ur-

    2006-09-01

    Full Text Available In the present work the antioxidant activity of different solvent (100% methanol, 80% methanol, 100% acetone, 80% acetone extracts of rice bran was evaluated following different antioxidant assays and using sunflower oil as oxidation substrate. The rice bran extracts were evaluated from the estimate of % inhibition of peroxidation in linoleic acid system, total phenolics content (TPC and loss of β-carotene in a linoleic acid system. Additionally, crude concentrated rice bran extracts were added into the sunflower oil samples and stored under ambient conditions. The extent of oxidative deterioration was followed by the measurement of peroxide-, p-anisidine-, conjugated diene-, and triene- values. The general order of antioxidant efficacy of rice bran extracts as determined by various antioxidant assays was 80% methanolic extract > 100% methanolic  extract > 80% acetone extract > 100% acetone extract. The results of the present comprehensive analysis demonstrate that rice bran extracts of the Super Kernel variety indigenous to Pakistan are a viable source of natural antioxidants and might be exploited for functional foods and nutraceutical applications.Se evalúa la actividad antioxidante diferentes extractos (100% metanol, 80% metanol, 100% acetona and 80% acetona de salvado de arroz -var. Super Kernel- mediante diferentes ensayos y utilizando aceite de girasol como substrato. Los ensayos utilizados fueron la estimación del % de inhibición de la peroxidación en sistemas con ácido linoleico, el contenido total en compuestos fenólicos y la pérdida de β-caroteno en sistemas con ácido linoleico. Adicionalmente, los concentrados de extractos de salvado de arroz se añadieron a aceite de girasol y las muestras se almacenaron a temperatura ambiente. La extensión de la oxidación se evaluó mediante el índice de peróxidos, el índice de p-anisidina, así como la formación de dienos y trienos conjugados. El orden de la eficacia antioxidante

  20. Antioxidant Activity, Colour and Mineral Content Of Herbal Tea Prepared From Cosmos Caudatus Leaves at Different Maturity Stages

    International Nuclear Information System (INIS)

    Dian Nashiela Fatanah; Noriham Abdullah; Nooraain Hashim; Azizah Abd Hamid

    2016-01-01

    In recent years, increasing consumption of herbal tea is a worldwide trend because of their refreshing taste, positive potential health effect, convenience, abundant resources and also cheaper in price. However, different maturity stages of plants being used as raw material could affect the antioxidant activity, colour and mineral content in herbal tea. Thus, this present study was aimed at determining antioxidant activity, colour and mineral content of herbal tea prepared from Cosmos caudatus (C. caudatus) leaves at three different maturity stages namely young, mature and old. All prepared herbal tea were analysed for total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), 2-2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, colour and mineral content. The results demonstrated that herbal tea prepared from young leaves had strong (p<0.05) antioxidant activity compared to mature and old leaves for all assays tested. Pearsons correlation coefficient also revealed that TPC and TFC exhibited a strong positive correlation with reducing power but negatively correlated with DPPH scavenging activity indicating that these compounds are major contributors to the antioxidant activity. In fact, the darker (p<0.05) colour of C. caudatus herbal tea prepared from young leaves relates to their higher antioxidant activity as compared to mature and old leaves. However, C. caudatus herbal tea prepared from old leaves showed significantly higher in mineral content compared to mature and young leaves. Hence, it can be concluded that, as maturity increased, antioxidant activity and colour in C. caudatus herbal tea decreased and vice-versa for mineral content. (author)

  1. Study on antioxidant activity of Echinacea purpurea L. extracts and ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-10-05

    Oct 5, 2009 ... This study investigates the antioxidant activity of Echinacea Purpurea L. (EP) extracts and its impact on cell viability. The polysaccharides content of EP was 159.8 ± 12.4 mg/g dry weight (DW), with extracts obtained by applying 55% ethanol at 55°C containing 11.0 ±1.0 mg gallic acid equivalent/g DW of ...

  2. Phytochemistry and antioxidant activity of Lallemantia iberica aerial parts

    Directory of Open Access Journals (Sweden)

    N. Khosravi Dehaghi

    2016-06-01

    Full Text Available Background and objectives:Lallemantia iberica (Lamiaceae is a medicinal plant distributed in different parts of Iran. This research, has evaluated the phytochemical constituents and antioxidant activity of the aerial parts of the plant. Methods: Different chromatographic methods such as column chromatographies using Silica gel (normal and reversed phases, Sephadex LH-20 and HPLC were used for isolation of the compounds from the ethyl acetate and methanol extract of L. iberica aerial parts. The structures of the isolated compounds were elucidated using 1H-NMR, 13C-NMR and EI-MS. Antioxidant activity of the extracts were also evaluated in DPPH and FRAP tests. Results: Two sterols,β-sitosterol acetate (1, β-sitosterol (2, one triterpenoic acid,ursolic acid (3, one polyphenol, rosmarinic acid (4 and six flavonoides,Luteolin-7-O-glucoside (5, 4'-methoxy-luteolin-7-O-glucoside (6, apigenin-7-O-glucoside (7, Luteolin (8,  diosmetin (9, apigenin (10 were isolated and identified from the ethyl acetate and methanol extracts. The antioxidant activity of the ethyl acetate (IC50 189.95±2.8 μg/mL and the methanol extracts (IC50 140±1.2 μg/mL were compared to the standard antioxidant, BHA (IC50 100±1.6 μg/mL in DPPH method. The reducing power of the ethyl acetate (300.28 μmol Eq FeSO4.7H2O/mg DW, the methanol extract (553.14 μmol Eq FeSO4.7H2O/mg DW and BHA (558.36 μmol Eq FeSO4.7H2O/mg of standard were elucidated in FRAP assay. Conclusion: The results introduce L. iberica as a medicinal plant with valuable constituents which are responsible of different pharmacological activities.

  3. Antioxidant activity and acute toxicity of Neoglaziovia variegata ...

    African Journals Online (AJOL)

    The most significant total phenolic content was of 543.50 ± 9.38 mg of gallic acid equivalent/g for ethyl acetate extract (AcOEt), which presented the best antioxidant activity (IC50 5.08 ± 0.20 μg/ml) for DPPH scavenging. The acute toxicity of Nv-EtOH was performed 2.0 g/kg intraperitoneally and 5.0 g/kg orally in mice.

  4. Variation of Antioxidant Activity and Phenolic Content of Some ...

    African Journals Online (AJOL)

    The antioxidant activity in short term air dried Ocimum sanctum plant methanol extract (IC50 = 0.03 ± 0.01 mg/ l) was the highest among the other plant extracts. However, Coriandrum sativum plant ethanol extract produced higher phenolic content (70.24±3.4/66.57±4.1 mg/g) than other plant extracts. It was found that there ...

  5. Phenolic compounds and antioxidant activities in some fruits and ...

    African Journals Online (AJOL)

    The highest values of TEAC were found in garlic (9.6 μmol), okra (3 μmol), spinach (2.2 μmol), tamarind (2.2 μmol) and onion (2.1 μmol). These data revealed that, some local fruits and vegetables from Burkina Faso are potential sources of bioactive compounds. Key words: Antioxidant activity, phenolic compounds, fruits, ...

  6. Soybean Extract Antioxidant Protective Activity Against Copper-Induced Cytotoxicity

    OpenAIRE

    Mansour I. Almansour

    2008-01-01

    The antioxidant activity of soybean crude extract against induced oxidative damage has been achieved through quails Coturnix coturnix treatment with various copper sulphate concentrations alone or with a protective dose of soybean crude extract. Several parameters of oxidative stress together with liver and kidney function tests in serum and liver tissue homogenate were studied. Hematologic indices and liver copper content were determined. Obtained data showed a significant increase in...

  7. Study on antioxidant activity of Echinacea purpurea L. extracts and ...

    African Journals Online (AJOL)

    This study investigates the antioxidant activity of Echinacea Purpurea L. (EP) extracts and its impact on cell viability. The polysaccharides content of EP was 159.8 ± 12.4 mg/g dry weight (DW), with extracts obtained by applying 55% ethanol at 55°C containing 11.0 ±1.0 mg gallic acid equivalent/g DW of total phenolic ...

  8. Antioxidant and antimicrobial activities of Bauhinia racemosa L. stem bark

    Directory of Open Access Journals (Sweden)

    Kumar R.S.

    2005-01-01

    Full Text Available The present study was carried out to evaluate the antioxidant and antimicrobial activities of a methanol extract of Bauhinia racemosa (MEBR (Caesalpiniaceae stem bark in various systems. 1,1-Diphenyl-2-picryl-hydrazyl (DPPH radical, superoxide anion radical, nitric oxide radical, and hydroxyl radical scavenging assays were carried out to evaluate the antioxidant potential of the extract. The antioxidant activity of the methanol extract increased in a concentration-dependent manner. About 50, 100, 250, and 500 µg MEBR inhibited the peroxidation of a linoleic acid emulsion by 62.43, 67.21, 71.04, and 76.83%, respectively. Similarly, the effect of MEBR on reducing power increased in a concentration-dependent manner. In DPPH radical scavenging assays the IC50 value of the extract was 152.29 µg/ml. MEBR inhibited the nitric oxide radicals generated from sodium nitroprusside with an IC50 of 78.34 µg/ml, as opposed to 20.4 µg/ml for curcumin. Moreover, MEBR scavenged the superoxide generated by the PMS/NADH-NBT system. MEBR also inhibited the hydroxyl radical generated by Fenton's reaction, with an IC50 value of more than 1000 µg/ml, as compared to 5 µg/ml for catechin. The amounts of total phenolic compounds were also determined and 64.7 µg pyrocatechol phenol equivalents were detected in MEBR (1 mg. The antimicrobial activities of MEBR were determined by disc diffusion with five Gram-positive, four Gram-negative and four fungal species. MEBR showed broad-spectrum antimicrobial activity against all tested microorganisms. The results obtained in the present study indicate that MEBR can be a potential source of natural antioxidant and antimicrobial agents.

  9. In vitro and in vivo antioxidant activities of polysaccharide purified from aloe vera (Aloe barbadensis) gel.

    Science.gov (United States)

    Kang, Min-Cheol; Kim, Seo Young; Kim, Yoon Taek; Kim, Eun-A; Lee, Seung-Hong; Ko, Seok-Chun; Wijesinghe, W A J P; Samarakoon, Kalpa W; Kim, Young-Sun; Cho, Jin Hun; Jang, Hyeang-Su; Jeon, You-Jin

    2014-01-01

    The in vitro and in vivo antioxidant potentials of a polysaccharide isolated from aloe vera gel were investigated. Enzymatic extracts were prepared from aloe vera gel by using ten digestive enzymes including five carbohydrases and five proteases. Among them, the highest yield was obtained with the Viscozyme extract and the same extract showed the best radical scavenging activity. An active polysaccharide was purified from the Viscozyme extract using ethanol-added separation and anion exchange chromatography. Purified aloe vera polysaccharide (APS) strongly scavenged radicals including DPPH, hydroxyl and alkyl radicals. In addition, APS showed a protective effect against AAPH-induced oxidative stress and cell death in Vero cells as well as in the in vivo zebrafish model. In this study, it is proved that both the in vitro and in vivo antioxidant potentials of APS could be further utilized in relevant industrial applications. Copyright © 2013 Elsevier Ltd. All rights reserved.

  10. Modification of the cellular antioxidant activity (CAA) assay to study phenolic antioxidants in a Caco-2 cell line.

    Science.gov (United States)

    Kellett, Mary E; Greenspan, Phillip; Pegg, Ronald B

    2018-04-01

    In vitro assays are widely used to analyze the antioxidant potential of compounds, but they cannot accurately predict antioxidant behavior in living systems. Cell-based assays, like the cellular antioxidant activity (CAA) assay, are gaining importance as they provide a biological perspective. When the CAA assay was employed to study phenolic antioxidants using hepatocarcinoma (HepG2) cells, quercetin showed antioxidant activity in HepG2 cells; 25 and 250μM quercetin reduced fluorescence by 17.1±0.9% and 58.6±2.4%, respectively. (+)-Catechin, a phenolic antioxidant present in many foods, bestowed virtually no CAA in HepG2 cells. When Caco-2 cells were employed, more robust antioxidant activity was observed; 50μM (+)-catechin and quercetin reduced fluorescence by 54.1±1.4% and 63.6±0.9%, respectively. Based on these results, likely due to differences in active membrane transport between the cell types, the Caco-2-based CAA assay appears to be a more appropriate method for the study of certain dietary phenolics. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Antioxidant and Hepatoprotective Activities of Flavonoids from Bauhinia hookeri

    Directory of Open Access Journals (Sweden)

    Eman Al-Sayed

    2016-05-01

    Full Text Available In a previous study, the total ethanol extract of Bauhinia hookeri showed a significant hepatoprotective effect in CCl 4-induced toxicity model in mice. However, the active components responsible for the activity were not identified. Therefore, this study was undertaken to determine if the activity of B. hookeri extract is due to its flavonoid content. The hepatoprotective activity of B. hookeri flavonoids was determined by measuring the activities of alanine aminotransferase (ALT and aspartate aminotransferase (AST in the culture medium of HepG2 cells challenged with CCl 4. The lipid peroxidation and antioxidant parameters, superoxide dismutase (SOD and glutathione (GSH were estimated in the cell lysates. The isolated flavonoids were identified by mass, UV and NMR spectral data. This study revealed that B. hookeri flavonoid fraction and its pure compounds ( kaempferol 3-O-β- D-glucoside, quercetin 3-O-β- D-glucoside and c atechin 3-O-α- L-rhamnoside possess a promising hepatoprotective activity as evidenced from the normalized levels of ALT and AST. This was attributed partly to their potent antioxidant activity as demonstrated by the increased GSH levels, SOD activity and reduced lipid peroxidation. The whole flavonoid fraction showed the highest cytoprotective activity and was more effective than silymarin. This study highlights a promising natural hepatoprotective remedy derived from B. hookeri.

  12. Antioxidant, Antigenotoxic, Antimicrobial Activities and Phytochemical Analysis of Dianthus carmelitarum

    Directory of Open Access Journals (Sweden)

    Rezzan Aliyazicioglu

    2017-05-01

    Full Text Available In this study, we investigated the phytochemical composition, antioxidant, antimicrobial, and antigenotoxic properties of the aqueous extract of Dianthus carmelitarum for the first time. The phenolic and volatile compounds, antioxidant, antimicrobial and antigenotoxic activities of the extract were determined by HPLC and SPME-GC-FID/MS, spectrophotometric, agar well diffusion methods and comet assay, respectively. The polyphenolic content and ferric reducing power values of the extract were found 12.6 ± 0.27 mg gallic acid and 238 ± 2.89 μM trolox equivalents per g sample, respectively. Syringaldehyde and chlorogenic acid were detected as major phenolic compounds, while terpenes were determined as major volatile compound. Dianthus carmelitarum extract especially exhibited moderate antimicrobial activity against Mycobacterium smegmatis. Extract reduced H 2O 2-induced DNA damage in a concentration dependent manner in fibroblast cells compared to positive control (only 20 μM H 2O 2 treatment. Dianthus carmelitarum can be considered in the food, cosmetic, and drug industries due to its antioxidant, antimicrobial, and antigenotoxic activities.

  13. Antioxidant activity of medicinal plant extracts in cookies.

    Science.gov (United States)

    Mišan, Aleksandra; Mimica-Dukić, Neda; Sakač, Marijana; Mandić, Anamarija; Sedej, Ivana; Simurina, Olivera; Tumbas, Vesna

    2011-01-01

    The antioxidant activity of ethanolic extracts of parsley, buckthorn, mint, caraway, and their mixture "Vitalplant" was evaluated, and the potential of "Vitalplant" mixture extract to retard the process of lipid oxidation in cookies was tested. The antioxidant activity was estimated by 2 direct (ESR) and 4 indirect (spectrophotometric) tests and correlated with the total phenolic and flavonoid content. The potential of "Vitalplant" mixture extract to retard the process of lipid oxidation in cookies was measured by thiobarbituric acid (TBA)-reactive-substances assay (TBARS) and DPPH˙ (1,1-diphenyl-2-picryl-hydrazyl) test. Significantly different (P buckthorn > "Vitalplant" > parsley > caraway. Total flavonoid content varied from 0.510% (parsley) to 2.05% ("Vitalplant"). A statistically significant correlation was found between IC(50) values on DPPH˙ and total flavonoid content of the samples (r=- 0.94, P cookies in dose-dependent manner. Supplementation of cookies with a mixture of Petroselini fructus, Frangulae cortex, Mentha piperitae folium, Carvi fructus can retard the process of lipid oxidation and elevate antioxidant activity of the final product. © 2011 Institute of Food Technologists®

  14. Antioxidant Activities of Polyphenols Extracted from Perilla frutescens Varieties

    Directory of Open Access Journals (Sweden)

    Linghua Meng

    2008-12-01

    Full Text Available Various cultivars of Perilla frutescens (L. (var. crispa and var. frutescens Britt. were harvested in China and Japan. They were easily differentiated on the basis of their foliage color, that varied from red to green. Water extracts of dried plants were investigated for their antioxidant activity (AA and their polyphenolic compounds compared. Among them, cinnamic acid derivatives (coumaroyl tartaric acid, caffeic acid and rosmarinic acid, flavonoids (apigenin 7-O-caffeoylglucoside, scutellarein 7-O-diglucuronide, luteolin 7-O-diglucuronide, apigenin 7-O-diglucuronide, luteolin 7-O-glucuronide, and scutellarein 7-O-glucuronide and anthocyanins (mainly cis-shisonin, shisonin, malonylshisonin and cyanidin 3-O-(E-caffeoylglucoside-5-O-malonylglucoside were quantified. AA assays are based on the inhibition of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH. The DPPH radical scavenging activity was calculated as Trolox ® [(±-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid] equivalent antioxidant capacity (TEAC. The mean amount of total phenolics of the water extracts (4-29 µmol/100 mL and the TEAC value calculated (23-167 µmol TE/100 mL confirmed the high antioxidant activity of these leaf water extracts. These results were highly correlated within some o-dihydroxylated polyphenolic compounds and AA.

  15. Mineral components and anti-oxidant activities of tropical seaweeds

    Science.gov (United States)

    Takeshi, Suzuki; Yumiko, Yoshie-Stark; Joko, Santoso

    2005-07-01

    Seaweeds are known to hold substances of high nutritional value; they are the richest resources of minerals important to the biochemical reactions in the human body. Seaweeds also hold non-nutrient compounds like dietary fiber and polyphenols. However, there is not enough information on the mineral compounds of tropical seaweeds. Also we are interested in the antioxidant activities of seaweeds, especially those in the tropical area. In this study, Indonesian green, brown and red algae were used as experimental materials with their mineral components analyzed by using an atomic absorption spectrophotometer. The catechins and flavonoids of these seaweeds were extracted with methanol and analyzed by high performance liquid chromatography (HPLC); the antioxidant activities of these seaweeds were evaluated in a fish oil emulsion system. The mineral components of tropical seaweeds are dominated by calcium, potassium and sodium, as well as small amounts of copper, iron and zinc. A green alga usually contains epigallocatechin, gallocatechin, epigallocatechin gallate and catechin. However, catechin and its isomers are not found in some green and red algae. In the presence of a ferrous ion catalyst, all the methanol extracts from the seaweeds show significantly lower peroxide values of the emulsion than the control, and that of a green alga shows the strongest antioxidant activity. The highest chelation on ferrous ions is also found in the extract of this alga, which is significantly different from the other methanol extracts in both 3 and 24 h incubations.

  16. Evaluating Antiproliferative and Antioxidant Activity of Marrubium crassidens

    Directory of Open Access Journals (Sweden)

    Sanaz Hamedeyazdan

    2014-10-01

    Full Text Available Purpose: Naturally occurring substances as novel drugs in cancer therapy, at all times, represent a challenge to science since medicinal plants are proving to be brilliant sources of new chemopreventive agents. Methods: In the present study, methanol extract from aerial parts of Marrubium crassidens was assessed for its antiproliferative activity in the breast cancer cell line MCF-7 through MTT bioassay using cell viability and cytotoxicity indices. The antioxidant property of M. crassidens extract together with its phenolic and flavonoids content were evaluated, as well. Results: According to data obtained in the study, M. crassidens exhibited antiproliferative activity with a gradual rise in cytotoxicty effect setting out on 240μg/mL concentration of the extract. Moreover, the RC50 value for antioxidant activity of the extract was determined as 40μg/mL and values for the total phenolic and flavonoids were calculated as 512.64mg gallic acid equivalent and 212.73mg quercetin equivalent per 100g of dry plant material. Conclusion: Generally, the observed antiproliferative and antioxidant properties of M. crassidens could be certified to the high amounts of phenolic and flavonoid content detected in the extract.

  17. Evaluating Antiproliferative and Antioxidant Activity of Marrubium crassidens.

    Science.gov (United States)

    Hamedeyazdan, Sanaz; Sharifi, Simin; Nazemiyeh, Hossein; Fathiazad, Fatemeh

    2014-10-01

    Naturally occurring substances as novel drugs in cancer therapy, at all times, represent a challenge to science since medicinal plants are proving to be brilliant sources of new chemopreventive agents. In the present study, methanol extract from aerial parts of Marrubium crassidens was assessed for its antiproliferative activity in the breast cancer cell line MCF-7 through MTT bioassay using cell viability and cytotoxicity indices. The antioxidant property of M. crassidens extract together with its phenolic and flavonoids content were evaluated, as well. According to data obtained in the study, M. crassidens exhibited antiproliferative activity with a gradual rise in cytotoxicty effect setting out on 240µg/mL concentration of the extract. Moreover, the RC50 value for antioxidant activity of the extract was determined as 40µg/mL and values for the total phenolic and flavonoids were calculated as 512.64mg gallic acid equivalent and 212.73mg quercetin equivalent per 100g of dry plant material. Generally, the observed antiproliferative and antioxidant properties of M. crassidens could be certified to the high amounts of phenolic and flavonoid content detected in the extract.

  18. Antifungal and antioxidant activity of Crassocephalum bauchiense (Hutch.) Milne-Redh ethyl acetate extract and fractions (Asteraceae).

    Science.gov (United States)

    Mouokeu, Raymond Simplice; Ngane, Rosalie A Ngono; Njateng, Guy S S; Kamtchueng, Monique O; Kuiate, Jules-Roger

    2014-04-17

    Crassocephalum bauchiense is a flowering plant, found in the West Region of Cameroon. Previous studied has highlighted the antibacterial and the dermal toxicological safety as well as the immunomodulatory activities of the ethyl acetate extract of its dry leaves. As an extension of the previous researches, the current work has been undertaken to evaluate the in vitro antifungal and antioxidant activities of C. bauchiense dried leaves ethyl acetate extract and fractions. The extract was obtained by maceration in ethyl acetate and further fractionated into six fractions labeled F1 to F6 by flash chromatography. The antifungal activity of the extract and fractions against yeasts and dermatophytes was evaluated using broth microdilution method. Antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) and β-carotene - linoleic acid assays. The extract (MIC = 0.125 - 4 mg/ml) was found to be more active on dermatophytes and yeasts compared to the fractions. The ethyl acetate extract and fractions exhibited strong scavenging activity on DPPH (CI50 = 28.57 - 389.38 μg/ml). The fractions F3 and F6 expressed best antioxidant activity on DPPH radicals compared to the crude extract. The results of these findings clearly showed that C. bauchiense ethyl acetate extract has a significant antifungal and antioxidant activity. It is therefore a source of active compounds that might be used as antifungal and antioxidant agents.

  19. In vitro antioxidant activities of the fractions of Coccinia grandis l. leaf ...

    African Journals Online (AJOL)

    The present study was aimed at investigating the antioxidant activities of the various fractions of the hydromethanolic extract of the leaves of Coccinia grandis L. Voigt. (Cucurbitaceae). The antioxidant activities of the fractions have been evaluated by using nine in vitro assays and were compared to standard antioxidants ...

  20. The antioxidants activity of flavonoids (procyanidol, quercetin, luteolin and kaempherol) in human lymphocytes exposed to ionizing radiation in vitro

    International Nuclear Information System (INIS)

    Stankovic, M.; Leskovac, A.; Petrovic, S.

    2007-01-01

    Phenolic components (flavonoids), may be important in prevention of DNA damage. We have tested the hypothesis that the flavonoids are protective against the DNA-damaging by γ-radiation in vitro. The study confirms strong antioxidant activity and radioprotective properties of flavonoids [sr