WorldWideScience

Sample records for spp falcata cv

  1. Medicago sativa spp. falcata

    African Journals Online (AJOL)

    STORAGESEVER

    2009-05-18

    May 18, 2009 ... Falcata (Medicago sativa spp. falcata L.), with its high resistance to cold weather, drought and disease, .... 40 plants. Seeds were grown in a greenhouse and seedlings were transplanted three months later to the experiment station in Hebei province ..... latitude range (from northern latitude 29° to northern.

  2. Deficiency symptoms of nitrogen, phosphorus, calcium and sulphur in pepper (Capsicum spp) cv malagueta

    International Nuclear Information System (INIS)

    Maruca, F.A.; Gouveia, P.R.; Teixeira, N.T.

    1989-01-01

    The pepper (Capsium spp) was cultivated in sand and nutritive solution to describe the deficiency symptoms of N, P, Ca and S. The deficiency symptoms and the first lack signals were clear to P and N nutrient. (author) [pt

  3. Ultrastructural changes and the distribution of arabinogalactan proteins during somatic embryogenesis of banana (Musa spp. AAA cv. 'Yueyoukang 1').

    Science.gov (United States)

    Pan, Xiao; Yang, Xiao; Lin, Guimei; Zou, Ru; Chen, Houbin; Samaj, Jozef; Xu, Chunxiang

    2011-08-01

    A better understanding of somatic embryogenesis in banana (Musa spp.) may provide a practical way to improve regeneration of banana plants. In this study, we applied scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to visualize the ultrastructural changes during somatic embryogenesis of banana (Musa AAA cv. 'Yueyoukang 1'). We also used histological and immunohistochemical techniques with 16 monoclonal antibodies to study the spatial distribution and cellular/subcellular localization of different arabinogalactan protein (AGP) components of the cell wall during somatic embryogenesis. Histological study with periodic acid-Schiff staining documented diverse embryogenic stages from embryogenic cells (ECs) to the late embryos. SEM revealed a mesh-like structure on the surface of proembryos which represented an early structural marker of somatic embryogenesis. TEM showed that ECs were rich in juvenile mitochondria, endoplasmic reticulum and Golgi stacks. Cells in proembryos and early globular embryos resembled ECs, but they were more vacuolated, showed more regular nuclei and slightly more developed organelles. Immunocytochemical study revealed that the signal of most AGP epitopes was stronger in starch-rich cells when compared with typical ECs. The main AGP component in the extracellular matrix surface network of banana proembryos was the MAC204 epitope. Later, AGP immunolabelling patterns varied with the developmental stages of the embryos. These results about developmental regulation of AGP epitopes along with developmental changes in the ultrastructure of cells are providing new insights into the somatic embryogenesis of banana. Copyright © Physiologia Plantarum 2011.

  4. Aroma Compounds Prevision using Artificial Neural Networks Influence of Newly Indigenous Saccharomyces SPP in White Wine Produced with Vitis Vinifera Cv Siria

    OpenAIRE

    Caldeira, A. Teresa; Martins, M. Rosário; Cabrita, Maria João; Ambrósio, Cristina; Arteiro, José; Neves, José; Vicente, Henrique

    2010-01-01

    Commercial yeasts strains of Saccharomyces cerevisae are frequently used in white wine production as starters in fermentation process, however, these strains can affect the wine characteristics. The aim of this study was to evaluate the effect of three strains of Saccharomyces spp. (var. 1, 2 and 3) on wine aroma compounds produced in microvinification assays. Microvinification assays were carried out with Vitis vinifera cv Síria grapes using the strains in study as starters. Aroma compounds ...

  5. Mineral fertilizer response and nutrient use efficiencies of East African highland banana (Musa spp., AAA-EAHB, cv. Kisansa)

    NARCIS (Netherlands)

    Nyombi, K.; Asten, van P.J.A.; Corbeels, M.; Taulya, G.; Leffelaar, P.A.; Giller, K.E.

    2010-01-01

    Poor yields of East African highland bananas (Musa spp., AAA-EAHB) on smallholder farms have often been attributed to problems of poor soil fertility. We measured the effects of mineral fertilizers on crop performance at two sites over two to three crop cycles; Kawanda in central Uganda and Ntungamo

  6. 40 CFR 180.1179 - Plant extract derived from Opuntia lindheimeri, Quercus falcata, Rhus aromatica, and Rhizophoria...

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Plant extract derived from Opuntia... Tolerances § 180.1179 Plant extract derived from Opuntia lindheimeri, Quercus falcata, Rhus aromatica, and... derived from Opuntia lindheimeri, Quercus falcata, Rhus aromatica, and Rhizophoria mangle is exempted from...

  7. Neuropharmacological and genotoxic evaluation of ethanol extract from Erythrina falcata leaves, a plant used in Brazilian folk medicine

    Directory of Open Access Journals (Sweden)

    Simone A. Dias

    2013-02-01

    Full Text Available The aim of the present study was to determine neurobehavioral and genotoxic activities of ethanol extract of Erythrina falcata Benth., Fabaceae, leaves on rats. Animals were treated with ethanol extract of E. falcata (100, 300 or 500 mg/kg; i.p. and the open field and elevated plus-maze tasks were used as behavioral models to investigate a possible effect on the locomotor and exploratory activity and anxiety, respectively. Genotoxic effect was investigated using the Comet assay. Ethanol extract of E. falcata leaves decreased the number of crossings and rearings in the open field task and increased the latency to start locomotion, though it was not able to affect habituation to apparatus measured 24h after the first session. Behavioral parameters in the plus-maze test were not affected by E. falcata. Ethanol extract did not increase damage index and damage frequency in blood or brain, indicating no genotoxic effect. The results suggest that ethanol extract of E. falcata leaves was able to affect locomotion, exploration, and motivation of animals without anxiolytic/anxiogenic effect, indicating a possible depressant action on the central nervous system. Furthermore, the lack of DNA damage in brain is an indicative that ethanol extract of E. falcata leaves may not induce neurotoxic effects.

  8. Neuropharmacological and genotoxic evaluation of ethanol extract from Erythrina falcata leaves, a plant used in Brazilian folk medicine

    Directory of Open Access Journals (Sweden)

    Simone A. Dias

    2013-04-01

    Full Text Available The aim of the present study was to determine neurobehavioral and genotoxic activities of ethanol extract of Erythrina falcata Benth., Fabaceae, leaves on rats. Animals were treated with ethanol extract of E. falcata (100, 300 or 500 mg/kg; i.p. and the open field and elevated plus-maze tasks were used as behavioral models to investigate a possible effect on the locomotor and exploratory activity and anxiety, respectively. Genotoxic effect was investigated using the Comet assay. Ethanol extract of E. falcata leaves decreased the number of crossings and rearings in the open field task and increased the latency to start locomotion, though it was not able to affect habituation to apparatus measured 24h after the first session. Behavioral parameters in the plus-maze test were not affected by E. falcata. Ethanol extract did not increase damage index and damage frequency in blood or brain, indicating no genotoxic effect. The results suggest that ethanol extract of E. falcata leaves was able to affect locomotion, exploration, and motivation of animals without anxiolytic/anxiogenic effect, indicating a possible depressant action on the central nervous system. Furthermore, the lack of DNA damage in brain is an indicative that ethanol extract of E. falcata leaves may not induce neurotoxic effects.

  9. Anxiolytic effects of Dolichandrone falcata Seem., Bignoniaceae, stem-bark in elevated plus maze and marble burying test on mice

    Directory of Open Access Journals (Sweden)

    Vishal B Badgujar

    2010-10-01

    Full Text Available Dolichandrone falcata Seem., Bignoniaceae, is a deciduous tree commonly known as Medshingi in local areas of Toranmal region of Maharashtra, India. Its bark paste is applied on fractured or dislocated bones, used as a fish poison; bark juice is used in cases of menorragia and leucorrhoea. The leaves of the plant have afforded chrysin-7-rutinoside. The present study was carried out to investigate the anxiolytic effects of methanol extract (DFBM, ethyl acetate extract (DFBEA and isolated compound DFB (V+VI of D. falcata stem-bark using animal models. Anxiolytic effects were studied by elevated plus maze (EPM and marble burying test (MBT assay. The crude dried DFBM and DFBEA extract was prepared in doses of 100, 200 and 400 mg/kg whereas DFB (V+VI compound was prepared in doses of 50, 100 and 200 mg/kg and were administered orally to mice for evaluation of anxiolytic activity. DFBEA 400 and DFB (V+VI 200 mg/kg produced highly significant (p <0.01 anxiolytic effects in dose dependent manner by increasing the time spent on and the number of entries into the open arms of the EPM and by decreasing the number of marbles buried by mice in MBT test. This study showed that the DFBM, DFBEA extracts and DFB (V+VI isolated compound possesses potential pharmacological active constituents flavonoids (like chrysin which may be responsible for the anxiolytic activity.

  10. The use of Euphorbia falcata extract as eco-friendly corrosion inhibitor of carbon steel in hydrochloric acid solution

    Energy Technology Data Exchange (ETDEWEB)

    El Bribri, A. [Laboratoire de Chimie Organique, Bioorganique et Environnement, Faculté des Sciences, Université Chouaib Doukkali, B.P. 20, M-24000 El Jadida (Morocco); Tabyaoui, M., E-mail: hamidtabyaoui@yahoo.fr [Laboratoire de Chimie Organique, Bioorganique et Environnement, Faculté des Sciences, Université Chouaib Doukkali, B.P. 20, M-24000 El Jadida (Morocco); Laboratoire des Matériaux, Nanoparticules et Environnement, Université Mohamed V Agdal, Faculté des Sciences, 4 Av. Ibn Battouta, B.P. 1014 RP, M-10000 Rabat (Morocco); Tabyaoui, B. [Laboratoire de Chimie Organique, Bioorganique et Environnement, Faculté des Sciences, Université Chouaib Doukkali, B.P. 20, M-24000 El Jadida (Morocco); El Attari, H.; Bentiss, F. [Laboratoire de Catalyse et de Corrosion des Matériaux (LCCM), Faculté des Sciences, Université Chouaib Doukkali, B.P. 20, M-24000 El Jadida (Morocco)

    2013-08-15

    Euphorbia falcata L. extract (EFE) was investigated as eco-friendly corrosion inhibitor of carbon steel in 1 M HCl using gravimetric, ac impedance, polarization and scanning electron microscopy (SEM) techniques. The experimental results show that EFE is good corrosion inhibitor and the protection efficiency is increased with the EEF concentration. The results obtained from weight loss and ac impedance studies were in reasonable agreement. Impedance experimental data revealed a frequency distribution of the capacitance, simulated as constant phase element. Polarization curves indicated that EFE is a mixed inhibitor. The corrosion inhibition was assumed to occur via adsorption of EFE molecules on the metal surface. The adsorption of the E. falcata extract was well described by the Langmuir adsorption isotherm. The calculated ΔG{sub ads}{sup o} value showed that the corrosion inhibition of the carbon steel in 1 M HCl is mainly controlled by a physisorption process. - Graphical abstract: Display Omitted - Highlights: • EFE is a good eco-friendly inhibitor for the corrosion of carbon steel in 1 M HCl. • EFE acts as mixed-type inhibitor in 1 M HCl medium. • Weight loss, ac impedance and polarization methods are in reasonable agreement. • The adsorption of EFE is well described by the Langmuir adsorption isotherm.

  11. Emgu CV essentials

    CERN Document Server

    Shi, Shin

    2013-01-01

    This book provides a practical guide to Emgu CV libraries, with sample code and examples used throughout to explain the concepts clearly. Each chapter deals with a different aspect of the Computer Vision field and the implementation of that topic in Emgu CV.If you are a C# programmer working on computer vision projects, this book is for you. You should have prior experience with C#.

  12. Avance al estudio radiológico del armamento de la necrópolis ibérica del Cabecico del Tesoro (Verdolay, Murcia. I. - Las Falcatas

    Directory of Open Access Journals (Sweden)

    García Cano, José Miguel

    2006-12-01

    Full Text Available

    We present a new study of the falcata swords decorated with silver inlay from the Iberian Iron Age cemetery at Cabecico del Tesoro (Murcia. Already known pieces are re-studied, while we also present new examples of previously unknown decorations, hidden under the corrosion layers but visible under radiographic examination.



    Se presenta un nuevo estudio de las falcatas decoradas con damasquinados en plata de la necrópolis ibérica del Cabecico del Tesoro (Murcia, Se reestudian piezas conocidas y se presentan nuevos casos de decoraciones previamente ocultas bajo la corrosión pero visibles en un examen radiológico efectuado sobre las piezas.

  13. Cloning and characterization of a novel stress-responsive WRKY transcription factor gene (MusaWRKY71) from Musa spp. cv. Karibale Monthan (ABB group) using transformed banana cells.

    Science.gov (United States)

    Shekhawat, Upendra K Singh; Ganapathi, Thumballi R; Srinivas, Lingam

    2011-08-01

    WRKY transcription factor proteins play significant roles in plant stress responses. Here, we report the cloning and characterization of a novel WRKY gene, MusaWRKY71 isolated from an edible banana cultivar Musa spp. Karibale Monthan (ABB group). MusaWRKY71, initially identified using in silico approaches from an abiotic stress-related EST library, was later extended towards the 3' end using rapid amplification of cDNA ends technique. The 1299-bp long cDNA of MusaWRKY71 encodes a protein with 280 amino acids and contains a characteristic WRKY domain in the C-terminal half. Although MusaWRKY71 shares good similarity with other monocot WRKY proteins the substantial size difference makes it a unique member of the WRKY family in higher plants. The 918-bp long 5' proximal region determined using thermal asymmetric interlaced-polymerase chain reaction has many putative cis-acting elements and transcription factor binding motifs. Subcellular localization assay of MusaWRKY71 performed using a GFP-fusion platform confirmed its nuclear targeting in transformed banana suspension cells. Importantly, MusaWRKY71 expression in banana plantlets was up-regulated manifold by cold, dehydration, salt, ABA, H2O2, ethylene, salicylic acid and methyl jasmonate treatment indicating its involvement in response to a variety of stress conditions in banana. Further, transient overexpression of MusaWRKY71 in transformed banana cells led to the induction of several genes, homologues of which have been proven to be involved in diverse stress responses in other important plants. The present study is the first report on characterization of a banana stress-related transcription factor using transformed banana cells.

  14. Doliroside A from Dolichos falcata Klein suppressing amyloid β-protein 42 fibrillogenesis: An insight at molecular level.

    Directory of Open Access Journals (Sweden)

    Dongpu Li

    Full Text Available A bioactive chemical constituent, doliroside A, from Chinese traditional herbal medicine Dolichos falcata Klein was isolated, purified and identified by 60% ethanol extraction, thin layer chromatography (TLC, high performance liquid chromatography (HPLC and nuclear magnetic resonance (NMR spectroscopy. Molecular interaction mechanism between doliroside and amyloid β42 protein was evaluated by thioflavin T fluorescence (ThT, circular dichroism (CD, atomic force microscope (AFM, and differential scanning calorimeter (DSC from the aspects of kinetics, secondary structure, morphology, and thermodynamics, respectively. Results show that the purity of doliroside A is 99.9% by HPLC, and its chemical structure is identified by 1H- and 13C-NMR. Doliroside A is observed to be concentration-dependent inhibiting the fibrillation of Aβ42 with the IC50 value of 26.57 ± 1.6 μM. CD and DSC results imply that doliroside A can bind to the nuclei and oligomers of Aβ42 to form a stable complex and suppress Aβ42 fibrillation. AFM images show that doliroside A, after bound to the nuclei and oligomers, redirect Aβ42 into off-pathway, amorphous oligomers. These findings not only provide a full insight into the molecular interaction mechanisms between Aβ42 and doliroside A, but also facilitate the development of new native anti-AD drug of doliroside A compound.

  15. Comparison of the phytoremediation potentials of Medicago falcata L. And Medicago sativa L. in aged oil-sludge-contaminated soil.

    Science.gov (United States)

    Panchenko, Leonid; Muratova, Anna; Turkovskaya, Olga

    2017-01-01

    Thirteen-year monitoring of the vegetation growing in the industrial and adjacent areas of an oil refinery showed the prevalence of yellow medick (Medicago falcata L.) over other plant species, including alfalfa (Medicago sativa L.). A comparative field study of the two Medicago species established that yellow medick and alfalfa exhibited similar resistance to soil petroleum hydrocarbons and that the pollutant concentration in their rhizosphere was 30% lower than that in the surrounding bulk soil. In laboratory pot experiments, yellow medick reduced the contaminant content by 18% owing to the degradation of the major heavy oil fractions, such as paraffins, naphthenes, and alcohol and benzene tars; and it was more successful than alfalfa. Both species were equally effective in stimulating the total number of soil microorganisms, but the number of hydrocarbon-oxidizing microorganisms, including polycyclic aromatic hydrocarbon degraders, was larger in the root zone of alfalfa. In turn, yellow medick provided a favorable balance of available nitrogen. Both Medicago species equally stimulated the dehydrogenase and peroxidase activities of the soil, and yellow medick increased the activity of soil polyphenol oxidase but reduced the activity of catalase. The root tissue activity of catalase, ascorbate oxidase, and tyrosinase was grater in alfalfa than in yellow medick. The peroxidase activity of plant roots was similar in both species, but nondenaturing polyacrylamide gel electrophoresis showed some differences in the peroxidase profiles of the root extracts of alfalfa and yellow medick. Overall, this study suggests that the phytoremediation potentials of yellow medick and alfalfa are similar, with some differences.

  16. Falcata ibérica con decoración damasquinada procedente del yacimiento de Cuesta del Espino (Córdoba

    Directory of Open Access Journals (Sweden)

    Sierra Montesinos, Manuel

    2006-12-01

    Full Text Available

    We would like to introduce an example of falcata, found by chance, which, in spite of not being completed at all, becomes very interesting due to its ornaments and also, due to know where it comes from for sure, from which we can deduce an arqueological approximation to the context, we believe funereal in which we must settle the piece down, as well as a first report on its formal and ornamental features.



    Presentamos un ejemplar de falcata aparecido de forma casual, que pese a estar incompleta resulta muy interesante por estar decorado y por conocer con bastante seguridad su lugar de procedencia, del cual se realiza una aproximación a su contexto arqueológico, creemos funerario, en que debe insertarse dicha pieza, así como un primer estudio de sus características formales y decorativas.

  17. Overexpression of MfPIP2-7 from Medicago falcata promotes cold tolerance and growth under NO3 (-) deficiency in transgenic tobacco plants.

    Science.gov (United States)

    Zhuo, Chunliu; Wang, Ting; Guo, Zhenfei; Lu, Shaoyun

    2016-06-14

    Plasma membrane intrinsic proteins (PIPs), which belong to aquaporins (AQPs) superfamily, are subdivided into two groups, PIP1 and PIP2, based on sequence similarity. Several PIP2s function as water channels, while PIP1s have low or no water channel activity, but have a role in water permeability through interacting with PIP2. A cold responsive PIP2 named as MfPIP2-7 was isolated from Medicago falcata (hereafter falcata), a forage legume with great cold tolerance, and transgenic tobacco plants overexpressing MfPIP2-7 were analyzed in tolerance to multiple stresses including freezing, chilling, and nitrate reduction in this study. MfPIP2-7 transcript was induced by 4 to 12 h of cold treatment and 2 h of abscisic acid (ABA) treatment. Pretreatment with inhibitor of ABA synthesis blocked the cold induced MfPIP2-7 transcript, indicating that ABA was involved in cold induced transcription of MfPIP2-7 in falcata. Overexpression of MfPIP2-7 resulted in enhanced tolerance to freezing, chilling and NO3 (-) deficiency in transgenic tobacco (Nicotiana tabacum L.) plants as compared with the wild type. Moreover, MfPIP2-7 was demonstrated to facilitate H2O2 diffusion in yeast. Higher transcript levels of several stress responsive genes, such as NtERD10B, NtERD10C, NtDREB1, and 2, and nitrate reductase (NR) encoding genes (NtNIA1, and NtNIA2) were observed in transgenic plants as compared with the wild type with dependence upon H2O2. In addition, NR activity was increased in transgenic plants, which led to alterations in free amino acid components and concentrations. The results suggest that MfPIP2-7 plays an important role in plant tolerance to freezing, chilling, and NO3 (-) deficiency by promoted H2O2 diffusion that in turn up-regulates expression of NIAs and multiple stress responsive genes.

  18. In vitro multiplication of banana (Musa sp.) cv. Grand Naine | Ahmed ...

    African Journals Online (AJOL)

    A micropropagation method is described for banana (Musa Spp.) Cv. Grand Naine. Suckers were surface sterilized with HgCl2 (0.1%) for 6 min which gave minimum contamination with maximum culture establishment. Of various treatment combinations, Murashige and Skoog (MS) medium + BAP 4.00 mg/l with IAA 2.00 ...

  19. Instant OpenCV starter

    CERN Document Server

    Dalal, Jayneil

    2013-01-01

    Get to grips with a new technology, understand what it is and what it can do for you, and then get to work with the most important features and tasks.A practical, quick, and hands-on guide for Python developers and hobbyists who want to get started with computer vision with OpenCV.This book is great for developers, hobbyists, and students new to computer vision who are looking to get a good grounding in how to use the OpenCV library. It's assumed that you will have some basic experience in C/C++ programming.

  20. Two myrmecophilous scale insects, Cryptostigma urichi (Cockerell (Hemiptera, Coccidae and Farinococcus multispinosus Morrison (Hemiptera, Pseudococcidae, cohabiting inside branches of Anadenanthera falcata (Benth. Speg. (Fabales, Fabaceae in the Cerrado area of São Paulo State, Brazil

    Directory of Open Access Journals (Sweden)

    André R. Nascimento

    2012-12-01

    Full Text Available The soft scale Cryptostigma urichi (Cockerell, 1894 and the mealybug Farinococcus multispinosus Morrison, 1922 are reported cohabiting inside branches of Anadenanthera falcata (Benth. Speg. (Angico-preto, both tended by the ant Azteca oecocordia Longino, 2007 (Hymenoptera, Formicidae. This interaction was observed in the Cerrado region sensu stricto, at the Federal University of São Carlos, UFSCar, Brazil. A. oecocordia is recorded for the first time in Brazil.

  1. Anxiolytic effects of Dolichandrone falcata Seem., Bignoniaceae, stem-bark in elevated plus maze and marble burying test on mice Efeitos ansiolíticos das cascas de Dolichandrone falcata Seem., Bignoniaceae, em teste do labirinto em cruz elevada e teste de esconder esferas, em camundongos

    Directory of Open Access Journals (Sweden)

    Vishal B Badgujar

    2010-11-01

    Full Text Available Dolichandrone falcata Seem., Bignoniaceae, is a deciduous tree commonly known as Medshingi in local areas of Toranmal region of Maharashtra, India. Its bark paste is applied on fractured or dislocated bones, used as a fish poison; bark juice is used in cases of menorragia and leucorrhoea. The leaves of the plant have afforded chrysin-7-rutinoside. The present study was carried out to investigate the anxiolytic effects of methanol extract (DFBM, ethyl acetate extract (DFBEA and isolated compound DFB (V+VI of D. falcata stem-bark using animal models. Anxiolytic effects were studied by elevated plus maze (EPM and marble burying test (MBT assay. The crude dried DFBM and DFBEA extract was prepared in doses of 100, 200 and 400 mg/kg whereas DFB (V+VI compound was prepared in doses of 50, 100 and 200 mg/kg and were administered orally to mice for evaluation of anxiolytic activity. DFBEA 400 and DFB (V+VI 200 mg/kg produced highly significant (p Dolichandrone falcata Seem., Bignoniaceae, é uma árvore do tipo decidua, comumente conhecida como "Medshingi" na da região Toranmal de Maharashtra, na Índia. Uma pasta da casca é aplicada em fraturas ou luxação dos ossos e usada como veneno de peixe; o suco da casca é usada em casos de menorragia e leucorréia. Das folhas da planta foi isolado crisina-7-rutinoside. O presente estudo foi realizado para investigar os efeitos ansiolíticos do extrato metanólico (DFBM, acetato de etila (DFBEA e compostos isolados DFB (V + VI de D. falcata utilizando a casca do tronco em modelos animais. Os efeitos ansiolíticos foram estudados por labirinto em cruz elevada (EPM e o ensaio de esconder esferas de mármore (MBT. Os extratos bruto e seco DFBM DFBEA foram preparados em doses de 100, 200 e 400 mg/kg, enquanto que o composto DFB (V + VI foi preparado em doses de 50, 100 e 200 mg/kg e foram administrados em camundongos para avaliação da atividade ansiolítica. DFBEA 400 and DFB (V+VI 200 mg/kg produziram efeitos

  2. C-V uniformity measurements

    International Nuclear Information System (INIS)

    Deming, R.O.; Keenan, W.A.

    1985-01-01

    Ion implantation's unique contributions to FET processing are excellent uniformity and repeatability at low dose. These could be verified on product wafers but were difficult to measure on monitor wafers. This paper presents an MOS technique that integrates the C-V curve directly to obtain part of the implanted dose - the partial dose. This technique is fast and insensitive to measurement noise and dot-diameter variation. It uses standard MOS wafers and processing. Neither photo-defined dots nor special substrates are required. Presently the integral CdV technique for partial dose is used in several FET lines to measure both implant repeatability and uniformity. (orig.)

  3. Characteristics of micro-propagated banana (Musa spp.) cultures ...

    African Journals Online (AJOL)

    Administrator

    2011-05-23

    May 23, 2011 ... was conducted to assess the effect of NaCl and PEG separately as well as in combination on plant micro- propagation efficiency of banana (Musa spp.) cv., Basrai. In this experiment, 4-weeks old plantlets of the 3rd sub- culture with well propagation on MS2b nutrient were sub- cultured on three differentially ...

  4. Acidithiobacillus caldus , Leptospirillum spp., Ferroplasma spp. and ...

    African Journals Online (AJOL)

    Acidithiobacillus caldus , Leptospirillum spp., Ferroplasma spp. and Sulphobacillus spp. mixed strains for use in cobalt and copper removal from water. ... Such findings suggest that if optimal conditions for biosorption of the metals by micro-organisms are achieved, this should afford a cost-effective method of removing metal ...

  5. INTERSTOCK EFFECT ON THE GROWTH OF MANDARIN CV BATU 55, TANGERINE CV PONTIANAK AND LIME CV NIMAS PROPAGATED BY GRAFTING

    Directory of Open Access Journals (Sweden)

    Sugiyatno A.

    2017-10-01

    Full Text Available Japansche citroen (JC rootstock is still the main choice for producing citrus seeds for its high availability and compatibility. Other rootstocks have been used in the production, yet they produce unsatisfying results. Based on the theory, there is an alternative to use rootstock as interstock in order to stimulate the growth of scion. The objective of this reseach is to understand the effect of interstock on the growth of Mandarin cv. Batu 55, Tangerine cv. Pontianak and Lime cv. Nimas. This research was conducted in the screen house of Tlekung Experimental Field of Indonesian Citrus and Subtropical Fruits Research Institute (ICSFRI in Batu-East Java, located on 950 m asl, from January to April 2016. This research was arranged in a CRD design with 4 replications and 24 interstock-scion combination treatments. The interstocks used in this study were Carizzo citrange, Citrumello, Poncirus trifoliata, Volkameriana, Rough lemon, Troyer citrange, Kanci and control (JC combined with 3 scions namely Mandarin cv. Batu 55, Tangerine cv. Pontianak and Lime cv. Nimas propagated by grafting. The result showed the percentage of successful grafting ranged between 37.5% to 100%, while the percentage of the shoot formed on grafted plants was 75%-100%. The use of Poncirus trifoliata as interstock has stimulated fastest bud break on Tangerine cv. Pontianak in just 24.75 days while the Troyer citrange has stimulated better shoot growth in Lime cv. Nimas, resulted to the highest shoot around 52.20 cm which was significantly different from other treatments. Meanwhile, Carizzo citrange has been found to have some influences to the plant diameter and the number of leave of Lime cv. Nimas but showed insignificant effect on the growth of its own diameter. The use of interstock showed that it can be used to promote the growth of scion which usually has slow growth when it is directly grafted on the rootstock.

  6. Wyoming CV Pilot Traveler Information Message Sample

    Data.gov (United States)

    Department of Transportation — This dataset contains a sample of the sanitized Traveler Information Messages (TIM) being generated by the Wyoming Connected Vehicle (CV) Pilot. The full set of TIMs...

  7. Nodulação e micorrização em Anadenanthera peregrina var. falcata em solo de cerrado autoclavado e não autoclavado Nodulation and mycorrhizal infection in Anadenanthera peregrina Var. falcata on autoclaved and non-autoclaved cerrado soil

    Directory of Open Access Journals (Sweden)

    E. Gross

    2004-02-01

    Full Text Available Anadenanthera peregrina var. falcata (angico-do-cerrado, uma leguminosa arbórea, forma associações simbióticas com bactérias fixadoras de nitrogênio (rizóbios e com fungos micorrízicos arbusculares. Com o objetivo de avaliar a eficiência da inoculação de fungos micorrízicos e rizóbios no crescimento inicial de plantas de angico-do-cerrado, crescidas em solo autoclavado e em solo não autoclavado com e sem inoculação, foi desenvolvido um experimento em casa de vegetação, utilizando raízes micorrizadas de milho e uma mistura de isolados de rizóbios como inoculantes. O crescimento das plantas foi influenciado positivamente pela concomitante inoculação do fungo micorrízico e do rizóbio, tendo as plantas desse tratamento apresentado biomassa cerca de 60 % maior do que o controle no décimo mês. A inoculação de apenas um dos microssimbiontes, entretanto, não provocou diferença na produção de biomassa das plantas. A percentagem de colonização micorrízica foi significativamente mais alta e o número de nódulos maior nas raízes das plantas crescidas no solo não autoclavado, ocasionados pela população de fungos e rizóbios nativos. Nesse tratamento, houve pequeno acúmulo de matéria no xilopódio, provavelmente em virtude do dreno fotossintético por parte dos microssimbiontes, e a concentração de P na parte aérea e xilopódio dessas plantas foi cerca de 1,2 e 8 vezes maior, respectivamente, por causa da colonização micorrízica.The leguminous tree Anadenanthera peregrina var. falcata (angico-do-cerrado forms symbiotic associations with nitrogen fixing bacteria (rhizobia and arbuscular mycorrhizal fungi. The aim of this study was the evaluation of the influence of rhizobial and arbuscular mycorrhizal inoculation on the initial growth of angico-do-cerrado plants, in autoclaved and non-autoclaved soil with and without inoculations. The experiment was carried out in a greenhouse using mycorrhized roots of maize and

  8. OpenCV for secret agents

    CERN Document Server

    Howse, Joseph

    2015-01-01

    This book is for programmers who want to expand their skills by building fun, smart, and useful systems with OpenCV. The projects are ideal in helping you to think creatively about the uses of computer vision, natural user interfaces, and ubiquitous computers (in your home, car, and hand).

  9. Armazenagem de morangos cv. Camarosa e cv. Verão em atmosfera modificada = Modified atmosphere storage of cv. Camarosa and cv. Verão strawberries

    Directory of Open Access Journals (Sweden)

    Renar João Bender

    2010-04-01

    Full Text Available A modificação da atmosfera de armazenagem é uma possibilidade de prolongar a vida pós-colheita de morangos. No presente trabalho, foram avaliadas quatro composições iniciais de atmosferas (3 kPa O2 + 10 kPa CO2; 5 kPa O2 + 15 kPa CO2; 21 kPa O2 + 15 kPa CO2; e ar ambiente em combinação com diferentes tempos de armazenagem. Morangos (Fragaria x ananassa Duch cv. Camarosa e Verão foram colhidos e colocados em embalagens de comercialização ainda nas lavouras. No galpão de embalamento, estas embalagens foram envoltas por sacos de polietileno com permeabilidade de 0,21 e 0,14 a 0,15 mL cm-2 hora-1 para O2 e para CO2, respectivamente. O emprego de atmosferas com concentrações iniciais de O2 e CO2, assim como o uso de polietileno contendo apenas ar ambiente, permite manter a qualidade de morangos cv. Camarosa por até seis dias de armazenagem a 1ºC. Morangos da cv. Verão não se beneficiaram da modificação deatmosfera a 4ºC. Os teores de sólidos solúveis totais, da acidez titulável e do ácido ascórbico, assim como a resistência à deformação, de morangos da cv. Camarosa não foram influenciados pelas combinações de atmosferas. Morangos da cv. Verão, no entanto, perderam significativamente firmeza de polpa e teores de ácido cítrico ao longo do período de armazenagem.Modified atmosphere storage is one of the possibilities to prolong the postharvest shelf life of strawberries. In the present work, four combinations of O2 and CO2 concentrations (3 kPa O2 + 10 kPa CO2; 5 kPa O2 + 15 kPa CO2; 21 kPa O2 + 15 kPa CO2; and ambient air were evaluated along various storage periods. Cv. Camarosa and cv. Verão strawberries (Fragaria x ananassa Duch were harvested from commercial groves and placed in trays and stored in low density polyethylene bags of 0.21 and 0.14 to 0.15 mL cm-2 hour-1 permeability to O2 and CO2, respectively. Modified atmospheres starting from known concentrations of O2 and CO2 or only ambient air maintain the

  10. The Limbic-Prefrontal Network Modulated by Electroacupuncture at CV4 and CV12

    Directory of Open Access Journals (Sweden)

    Jiliang Fang

    2012-01-01

    Full Text Available fMRI studies showed that acupuncture could induce hemodynamic changes in brain networks. Many of these studies focused on whether specific acupoints could activate specific brain regions and were often limited to manual acupuncture at acupoints on the limbs. In this fMRI study, we investigated acupuncture's modulation effects on brain functional networks by electroacupuncture (EA at acupoints on the midline of abdomen. Acupoints Guanyuan (CV4 and Zhongwan (CV12 were stimulated in 21 healthy volunteers. The needling sensations, brain activation, and functional connectivity were studied. We found that the limbic-prefrontal functional network was deactivated by EA at CV4 and CV12. More importantly, the local functional connectivity was significantly changed during EA stimulation, and the change persisted during the period after the stimulation. Although minor differences existed, both acupoints similarly modulated the limbic-prefrontal functional network, which is overlapped with the functional circuits associated with emotional and cognitive regulation.

  11. Assessing the Pathogenic Ability of Ralstonia pseudosolanacearum (Ralstonia solanacearum Phylotype I from Ornamental Rosa spp. Plants

    Directory of Open Access Journals (Sweden)

    Napoleon N. A. Tjou-Tam-Sin

    2017-11-01

    Full Text Available Ralstonia pseudosolanacearum (Ralstonia solanacearum phylotype I isolates found in stunted, yellowing, and wilted ornamental rose (Rosa spp. were assessed for their pathogenic ability in two rose cultivars (cv. “Armando” and cv. “Red Naomi” and in four solanaceous crops: tomato (Solanum lycopersicum cv. “Money Maker”, tobacco (Nicotiana tabacum cv. “White Burley”, eggplant (Solanum melongena cv. “Black Beauty” and sweet pepper (Capsicum annum cv. “Yolo Wonder”. Significant differences were observed in susceptibility between the two rose cultivars as well as between the two modes of inoculation performed. The cultivar “Armando” was significantly more susceptible than cultivar “Red Naomi,” exhibiting higher disease severity and incidence. Similarly, stem inoculation after wounding was found to be significantly more effective than soil drenching, resulting in higher disease severity. Additionally, a temperature dependency in susceptibility was observed for both cultivars irrespective of the mode of inoculation, however, this was significantly more pronounced upon soil drenching. The solanaceous crops all showed to be susceptible to the R. pseudosolanacearum isolates originated from the Rosa spp. plants. Furthermore, both rose cultivars were able to harbor symptomless infections with other R. pseudosolanacearum and R. solanacearum isolates than those isolated from rose. Our results clearly demonstrated that latent infections in a rose cultivar such as cv. “Red Naomi” do occur even at temperatures as low as 20°C. This latency poses high risks for the entire floricultural industry as latently infected Rosa spp. plants are propagated and distributed over various continents, including areas where climatic conditions are optimal for the pathogen.

  12. CV controls from design to operation

    CERN Document Server

    Blanc, D

    2002-01-01

    The cooling and Ventilation (CV) group has emphasised the need to redefine its organisational structure at the end of 98. The main objective of this operation was to ensure the CV group to be more competitive and efficient through the growing tasks of the LHC projects. The main evolution given to this reorganisation is that the new structure is more project oriented and then operates on three distinct axes: Design, Work and Operation. Process control project management requires a complete and early interaction and participation of all the actors involved. This procedure to be efficient and constructive must be considered and performed not only during the design stage but along the project planning phases and must go beyond the completion work including the process control operation activity. The paper explains the present project management for process control. It describes the present constraints and gives suggestions to a different approach to these projects to improve performances and efficiency of a contr...

  13. EARTH, MOON, SUN, AND CV ACCRETION DISKS

    International Nuclear Information System (INIS)

    Montgomery, M. M.

    2009-01-01

    Net tidal torque by the secondary on a misaligned accretion disk, like the net tidal torque by the Moon and the Sun on the equatorial bulge of the spinning and tilted Earth, is suggested by others to be a source to retrograde precession in non-magnetic, accreting cataclysmic variable (CV) dwarf novae (DN) systems that show negative superhumps in their light curves. We investigate this idea in this work. We generate a generic theoretical expression for retrograde precession in spinning disks that are misaligned with the orbital plane. Our generic theoretical expression matches that which describes the retrograde precession of Earths' equinoxes. By making appropriate assumptions, we reduce our generic theoretical expression to those generated by others, or to those used by others, to describe retrograde precession in protostellar, protoplanetary, X-ray binary, non-magnetic CV DN, quasar, and black hole systems. We find that spinning, tilted CV DN systems cannot be described by a precessing ring or by a precessing rigid disk. We find that differential rotation and effects on the disk by the accretion stream must be addressed. Our analysis indicates that the best description of a retrogradely precessing spinning, tilted, CV DN accretion disk is a differentially rotating, tilted disk with an attached rotating, tilted ring located near the innermost disk annuli. In agreement with the observations and numerical simulations by others, we find that our numerically simulated CV DN accretion disks retrogradely precess as a unit. Our final, reduced expression for retrograde precession agrees well with our numerical simulation results and with selective observational systems that seem to have main-sequence secondaries. Our results suggest that a major source to retrograde precession is tidal torques like that by the Moon and the Sun on the Earth. In addition, these tidal torques should be common to a variety of systems where one member is spinning and tilted, regardless if

  14. Learning openCV computer vision with the openCV library

    CERN Document Server

    Bradski, Gary

    2008-01-01

    Learning OpenCV puts you right in the middle of the rapidly expanding field of computer vision. Written by the creators of OpenCV, the widely used free open-source library, this book introduces you to computer vision and demonstrates how you can quickly build applications that enable computers to see" and make decisions based on the data. With this book, any developer or hobbyist can get up and running with the framework quickly, whether it's to build simple or sophisticated vision applications

  15. Improved tolerance toward fungal diseases in transgenic Cavendish banana (Musa spp. AAA group) cv. Grand Nain.

    Science.gov (United States)

    Vishnevetsky, Jane; White, Thomas L; Palmateer, Aaron J; Flaishman, Moshe; Cohen, Yuval; Elad, Yigal; Velcheva, Margarita; Hanania, Uri; Sahar, Nachman; Dgani, Oded; Perl, Avihai

    2011-02-01

    The most devastating disease currently threatening to destroy the banana industry worldwide is undoubtedly Sigatoka Leaf spot disease caused by Mycosphaerella fijiensis. In this study, we developed a transformation system for banana and expressed the endochitinase gene ThEn-42 from Trichoderma harzianum together with the grape stilbene synthase (StSy) gene in transgenic banana plants under the control of the 35S promoter and the inducible PR-10 promoter, respectively. The superoxide dismutase gene Cu,Zn-SOD from tomato, under control of the ubiquitin promoter, was added to this cassette to improve scavenging of free radicals generated during fungal attack. A 4-year field trial demonstrated several transgenic banana lines with improved tolerance to Sigatoka. As the genes conferring Sigatoka tolerance may have a wide range of anti-fungal activities we also inoculated the regenerated banana plants with Botrytis cinerea. The best transgenic lines exhibiting Sigatoka tolerance were also found to have tolerance to B. cinerea in laboratory assays.

  16. Propagación in vitro de semillas de la orquídea Comparettia falcata Poepp. & Endl. (Orchidaceae mediante técnicas simbióticas y asimbióticas

    Directory of Open Access Journals (Sweden)

    Héctor Karol Chávez

    2015-04-01

    Full Text Available Para su germinación, las orquídeas dependen de hongos micorrízicos y muchas de las características únicas de estas plantas están asociadas con el hongo que las coloniza. En este trabajo se evaluó la germinación de semillas de la orquídea Comparettia falcata en condiciones de laboratorio utilizando los métodos simbióticos: dos hongos micorrízicos de orquídeas de diferentes especies y un aislamiento patogénico de Rhizoctonia solani obtenido de arroz, y los asimbioticos: formulación de Knudson C y MS (Murashie & Skood. Se encontraron diferencias (P < 0.05 entre tratamientos, resultando mejor el método asimbiótico con la formulación Knudson C y el simbiótico con R. solani, patógeno de arroz. Además, se observó que los tratamientos donde se implementó la metodología simbiótica tuvieron menor porcentaje de contaminación por microorganismos que los tratamientos de tipo asimbiótico.

  17. Organisation des travaux ST-CV

    CERN Document Server

    Martel, C; CERN. Geneva. ST Division

    2003-01-01

    Débutée il y a deux ans, la phase de construction des installations de refroidissement du LHC a atteint en 2003 son apogée. De nombreux autres projets sont également gérés par le groupe dans divers secteurs du laboratoire. La spécificité du groupe est de réaliser des installations complexes impliquant des spécificités diverses telles qu’aéraulique, hydraulique, électrique, etc. Pour faire face à ces demandes la section travaux du groupe ST CV a adopté une structure capable de réaliser ces divers projets avec un minimum de personnel. La première partie de ce document décrit l’organisation de la section travaux ST CV. Dans une deuxième partie, les auteurs dressent un état des lieux des chantiers par projet en décrivant plus particulièrement les activités dans les domaines du traitement d’air et de refroidissement par eau.

  18. Uji Infeksi Mycosphaerella spp Terhadap Bibit Eucalyptus spp

    OpenAIRE

    Lidya Morita Sondang

    2009-01-01

    Tujuan penelitian ini adalah untuk mengetahui tingkat ketahanan 2 klon Eucalyptus spp yaitu Eucalyptus grandis x Eucalyptus pellita dan Eucalyptus grandis x Eucalyptus urophylla terhadap Mycosphaerella spp serta mengetahui virulensi Mycospaherella spp pada 2 kelas umur (2 dan 3 bulan) pada tanaman Eucalyptus spp. Penelitian ini dilaksanakan dengan pengambilan sampel bibit tanaman Eucalyptus grandis x Eucalyptus pellita dan Eucalyptus grandis x Eucalyptus urophylla dari pembibitan PT.Toba Pulp...

  19. Cloning and sequencing of Duck circovirus (DuCV).

    Science.gov (United States)

    Hattermann, K; Schmitt, C; Soike, D; Mankertz, A

    2003-12-01

    The genome of Duck circovirus (DuCV) is circular and 1996 nts in size. Two major open reading frames were identified, encoding the replicase (V1) and the capsid protein (C1). A stem-loop structure comprising the nonamer 5'-TATTATTAC, conserved in all circo-, nano- and geminiviruses, was found. Unique to DuCV, the region between the 3'-ends of the rep and cap gene contains four repeats of a 44-bp sequence. Phylogenetic analysis shows close relation of DuCV with Goose circovirus and suggests classification of DuCV as a new member of the genus Circovirus of the virus family Circoviridae.

  20. Using microsatellite (SSR) and morphological markers to assess the ...

    African Journals Online (AJOL)

    Falcata (Medicago sativa spp. falcata L.), with its high resistance to cold weather, drought and disease, plays an important role in alfalfa breeding. The aim of this study was to assess the genetic diversity of the 12 falcata populations in Eurasia using SSR markers and morphological traits. Regressions for genetic distance, ...

  1. due to Klebsiella spp.

    African Journals Online (AJOL)

    This was a prospective study undertaken at Hillbrow. Hospital in Johannesburg during a 3-month period. All patients from whose sputum cultures Klebsiella spp. were isolated in significant growth on primary blood agar plates following culture of fresh sputum specimens were entered into the study (2+ grO\\:vth was equal to.

  2. In vitro micropropagation of almond (Amygdalus communis L. cv ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-06-17

    Jun 17, 2008 ... This method here in described will be useful for the rapid multiplication of almond (A. communis L. cv. Nonpareil) in commercial exploitation. Key words: Amygdalus communis L.cv. Nonpareil, micropropagation, zygotic embryos, in vitro. INTRODUCTION. The almond (Prunus dulcis, syn. Prunus amygdalus ...

  3. C-V Test Structures for Metal Gate CMOS

    NARCIS (Netherlands)

    Bankras, R.G.; Tiggelman, M.P.J.; Negara, M. Adi; Sasse, G.T.; Schmitz, Jurriaan

    2006-01-01

    Gate leakage has complicated the layout and measurement of C-V test structures. In this paper the impact of metal gate introduction to C-V test structure design is discussed. The metal gate allows for wider-gate structures and for the application of n+-p+ diffusion edges. We show, both theoretically

  4. A practical introduction to computer vision with OpenCV

    CERN Document Server

    Dawson-Howe, Kenneth

    2014-01-01

    Explains the theory behind basic computer vision and provides a bridge from the theory to practical implementation using the industry standard OpenCV libraries Computer Vision is a rapidly expanding area and it is becoming progressively easier for developers to make use of this field due to the ready availability of high quality libraries (such as OpenCV 2).  This text is intended to facilitate the practical use of computer vision with the goal being to bridge the gap between the theory and the practical implementation of computer vision. The book will explain how to use the relevant OpenCV

  5. IDENTIFICATION OF DIFFERENT FUSARIUM SPP. IN ALLIUM SPP. IN GERMANY.

    Science.gov (United States)

    Boehnke, B; Karlovsky, P; Pfohl, K; Gamliel, A; Isack, Y; Dehne, H W

    2015-01-01

    In 2013 Allium cepa bulbs from different fields in Northern and Southern Germany, seeds and sets from onion breeders were analysed for infestation with Fusarium species. The same investigation was done in 2014 with different edible Allium spp. from local markets. Different Fusarium spp. were isolated and identified by morphological characterisation. 24 different Fusarium spp. were identified. The diversity of Fusarium spp. and the intensity of infestation was higher on edible bulbs compared to the younger sets and seeds. The analysed onions and other edible Allium spp. from local markets showed also high contents of different Fusarium species. The most prevalent identified Fusarium sp. in the analysed Allium spp. in Germany was Fusarium oxysporum which can cause the Fusarium Basal Rot, followed by Fusarium solani. Fusarium proliferatum, which can cause the Fusarium Salmon Blotch in onions, could be detected in about half of the sampled onion fields and in approximately 10% of all analysed onions from fields. Also in the onion sets, on the surface of the seeds and in other edible Allium spp. F. proliferatum could be identified. Besides F. proliferatum, further mycotoxin producing Fusarium spp. like Fusarium equiseti or Fusarium tricinctum were identified. Other Fusarium spp. like Fusarium sporotrichioides and Fusarium poae were first described in Allium sp. in this study. The two most prevalent Fusarium spp. F. oxysporum and F. solani are able to produce mycotoxins like enniatins, fumonisins, moniliformin and T-2 toxins. Fusarium sp. like F. proliferatum, F. equiseti and F. tricinctum are able to produce additional toxins like beauvericins, zearalenone and diacetoscirpenol. This high number of Fusarium spp., which are able to produce a broad spectrum of different mycotoxins, could be a potential health risk for human beings and livestock.

  6. Guidelines on CV networking information flow optimization for Texas.

    Science.gov (United States)

    2017-03-01

    Recognizing the fundamental role of information flow in future transportation applications, the research team investigated the quality and security of information flow in the connected vehicle (CV) environment. The research team identified key challe...

  7. Calibration of ADRET voltage generator type CV102. Program CODAV

    International Nuclear Information System (INIS)

    Lagarde, Gerard.

    1978-07-01

    The CODAV programm studied by the Metrology SES/SME laboratory is used for the calibration of ADRET voltage generator type CV.102. A JCAM.10 microcomputer run the measurement cycle and the printout of the results [fr

  8. CV pilot deployment concept phase 1, outreach plan -- ICF Wyoming.

    Science.gov (United States)

    2016-06-24

    The Wyoming Department of Transportations (WYDOT) Connected Vehicle (CV) Pilot Deployment Program is intended to develop a suite of applications that utilize vehicle to infrastructure (V2I) and vehicle to vehicle (V2V) communication technology to ...

  9. Uso eficiente del agua para el cultivo de rosa cv. Freedom bajo invernadero

    Directory of Open Access Journals (Sweden)

    John J. Arévalo-H

    2013-08-01

    Full Text Available Determinar el requerimiento de agua y el momento adecuado de distribución, es un factor fundamental para el cultivo de flores. El objetivo del presente trabajo fue determinar el efecto de la aplicación de tres láminas de riego sobre el desarrollo y producción del cultivo de la Rosa cv. Freedom, bajo invernadero. El experimento se realizó con tres láminas de riego en un cultivo de rosa (Rosa spp. cv. Freedom. Se analizó el crecimiento de los tallos florales, la vida en florero, la producción y la eficiencia en el uso del agua. Los tratamientos se basaron en tres niveles de evapotranspiración de cultivo (ETc (70, 80 y 100%. Durante el estudio, el cultivo se mantuvo en producción constante. Se aplicaron 384,87, 254,25 y 218,97 m³ correspondiente a 100, 80 e 70% de ETc respectivamente, significando un ahorro de agua de 14,0 y 21,8% en el 80 e 70% de ETc respectivamente. El coeficiente de cultivo (Kc vario entre 0,58 y 1,65, con una media de 1,13. No se encontraron diferencias en la producción. Las curvas de crecimiento presentaron incremento continuo, con ajuste al modelo logístico. El 70% de ETc presentó la mayor eficiencia en el uso del agua, con 185 tallos m-3. La conservación media de los tallos florales fue de 12 a 15 días. La mayor causa de pérdida fue la deshidratación, que inició en el día 11, comportamiento normal del tallo floral.

  10. DNA analysis in three populations of African spinach (Basella spp.)

    International Nuclear Information System (INIS)

    Grasso, G.; Van Duren, M.; Lee, K.S.; Morpurgo, R.

    1997-01-01

    African spinach (Basella spp.) is an important vegetable in West Africa, and was introduced by early colonialists. Its alien origin is supported by its narrow genetic variability. Flowcytometry and RAPD polymorphism were used to investigate genetic variation in three populations of Basella - 'Congo native', 'Cong domesticated', and an introduced cultivar, 'Sri Lanka' from Sri Lanka. Normal spinach (Spinacia oleracea) cv. 'Prince F 1 Hybrid' was used to test sensitivity and to verify detection of genetic variation. Nuclei were isolated from young leaves of Basella, stained with DAPI and ethidium bromide, and ploidy level and total DNA content were determined by using a flowcytometer. The two sexually propagated populations, 'Cong domesticated' and 'Sri Lanka' showed very low amount of genetic variation as revealed by RAPD analysis; the third population 'Congo native' showed a limited amount of polymorphism. (author). 8 refs, 1 fig., 2 tabs

  11. Anonymous voting for multi-dimensional CV quantum system

    International Nuclear Information System (INIS)

    Shi Rong-Hua; Xiao Yi; Shi Jin-Jing; Guo Ying; Lee, Moon-Ho

    2016-01-01

    We investigate the design of anonymous voting protocols, CV-based binary-valued ballot and CV-based multi-valued ballot with continuous variables (CV) in a multi-dimensional quantum cryptosystem to ensure the security of voting procedure and data privacy. The quantum entangled states are employed in the continuous variable quantum system to carry the voting information and assist information transmission, which takes the advantage of the GHZ-like states in terms of improving the utilization of quantum states by decreasing the number of required quantum states. It provides a potential approach to achieve the efficient quantum anonymous voting with high transmission security, especially in large-scale votes. (paper)

  12. Potensi Trichoderma Spp. Sebagai Agens Pengendali Fusarium Spp. Penyebab Penyakit Layu Pada Tanaman Stroberi

    OpenAIRE

    Dwiastuti, Mutia Erti; Fajri, Melisa N; Yunimar, Yunimar

    2015-01-01

    Layu yang disebabkan oleh Fusarium spp. merupakan salah satu penyakit penting tanaman stroberi (Fragaria x ananassa Dutch.) di daerah subtropika, yang dapat menggagalkan panen. Penelitian bertujuan untuk mempelajari potensi Trichoderma spp. dalam mengendalikan Fusarium spp. Isolat Trichoderma spp. diisolasi dari rizosfer tanaman stroberi dan Fusarium spp. diisolasi dari tanaman stroberi yang mengalami layu fusarium. Isolat cendawan dimurnikan, dikarakterisasi, dan dibandingkan dengan isolat c...

  13. Instant OpenCV for iOS

    CERN Document Server

    Kornyakov, Kirill

    2013-01-01

    Filled with practical, step-by-step instructions and clear explanations for the most important and useful tasks. This book uses a very practical approach, with each recipe and their associated sample projects or examples focusing on a particular aspect of the technology.This book is intended for OpenCV developers who are interested in porting their applications to the iOS platform. Basic experience with OpenCV, computer vision, Objective C, and other iOS tools is encouraged.

  14. Ensacamento de frutos de pereira cv. Housui Bagging of nashi pear cv. Housui

    Directory of Open Access Journals (Sweden)

    Ivan Dagoberto Faoro

    2004-04-01

    Full Text Available Foi avaliada a qualidade de frutos de pêra japonesa cv. Housui (Pyrus pyrifolia var. culta ensacados com diferentes tipos de sacos de papel e em duas épocas: 34 e 83 dias após a florada. O ensacamento não influenciou na firmeza, no teor de sólidos solúveis totais e no peso médio dos frutos. Sacos vermelhos não induziram aumento do pH da polpa, mas o uso de sacos duplos, sacos marrons, sacos de papel kraft marrons e sacos de pipoca brancos aumentaram significativamente o pH. Ao buscar-se maior precocidade de colheita, os melhores resultados foram obtidos com o uso de sacos pequenos de papel manteiga aos 34 dias após a floração e o uso de sacos grandes duplos ou sacos grandes marrons, 83 dias após a plena floração. O ensacamento 34 dias após a plena floração, com sacos grandes de papel duplo de cor marrom ou sacos de papel kraft marrons, ou ainda o uso de sacos pequenos parafinados transparentes de papel manteiga, aos 34 dias, seguidos pela colocação, aos 83 dias, dos dois tipos de sacos grandes citados anteriormente, resultaram em frutos de melhor qualidade externa (película de coloração homogênea e mais clara, lisa e com lenticelas pouco salientes. O uso de sacos vermelhos de papel manteiga e de sacos de pipoca brancos, com ou sem ensacamento prévio com sacos pequenos de papel manteiga parafinado, não resultaram em melhoria substancial da qualidade externa do fruto.The fruit quality of Nashi pears cv. Housui (Pyrus pyrifolia var. culta have been evaluated after bagging with different paper bags at two bagging dates: 34 and 83 days after full bloom. The bagging did not affect the fruit firmness, the total soluble solids and the average fruit weight. Red bags did not affect the pH of the fruit flesh. However the fruit bagged with double bags of brown color, brown kraft paper and white popcorn paper increased the pH. In order to harvest precociously, the use of small transparent paraffin paper bags 34 days after full bloom

  15. Agrobacterium mediated transformation of banana ( Musa sp.) cv ...

    African Journals Online (AJOL)

    Five lines were then selected on the basis of putative PCR positives and a Southern blot analysis gave hybridization signals with 1 to 4 copy number integration patterns characteristic of Agrobacterium mediated transformation. These results confirm stable gene integration in East African banana cultivar cv. Sukali Ndiizi ...

  16. The early-type close binary CV Velorum revisited

    NARCIS (Netherlands)

    Yakut, K.; Aerts, C.C.; Morel, T.

    2007-01-01

    Aims.Our goal was to improve the fundamental parameters of the massive close double-lined eclipsing B2.5V+B2.5V binary CV Velorum. Methods: We gathered new high-resolution échelle spectroscopy on 13 almost consecutive nights covering two orbits. We computed a simultaneous solution to all the

  17. An evaluation of Panicum maximum cv. Gatton: 3. The partial ...

    African Journals Online (AJOL)

    NDF disappearance from the total tract decreased with increasing pasture maturity during all seasons. It was concluded that P. maximum cv. Gatton pasture would best be utilized at the younger stages of development and that nutritional value would be higher in summer followed by younger autumn and winter pastures.

  18. Micropropagation of Anthurium andreanum cv. Terra | Farsi | African ...

    African Journals Online (AJOL)

    Anthurium anderanum cv. Terra from family Araceae, is a plant with a high commercial value. Conventional propagation of this plant performed via suckers and seeds planting that nowadays missed its application have been abolished. This study aimed to establish producible protocol for indirect in vitro regeneration of the ...

  19. Plant regeneration of Brassica oleracea subsp. italica (Broccoli) CV ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-06-03

    Jun 3, 2009 ... Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul. Ehsan, Malaysia. Accepted 20 March, 2009. Hypocotyls and shoot tips were used as explants in in vitro plant regeneration of broccoli (Brassica oleracea subsp.italica) cv. Green Marvel.

  20. Chemical control of weeds during the acclimatization of in vitro sugarcane plants cv. 'CP52-43'

    Directory of Open Access Journals (Sweden)

    Inoel García Ruiz

    2016-07-01

    Full Text Available Control of weeds in the acclimatization phase of in vitro sugarcane (Saccharum spp. plants is done manually which raises the cost of production. In order to determine the effectiveness of the chemical control of weeds with Dual Gold CE 96 (Mesotrione, in vitro plants of sugar cane cv. 'CP52-43' were acclimatized in worm humus and cachaça compost. Doses of 0.5, 1.0 and 1.5 l ha-1 p.c. Dual Gold CE 96 herbicide, were applied before and immediately to in vitro plants transplantation and the results were compared to manual weeding. After 50 days of culture, 15 plants were extracted per treatment and the number of leaves and shoots were quantified, the length was measured and the fresh mass of the aerial part and of the roots were determined. The results showed that Dual Gold CE 96 at doses of 0.5 l ha-1 applied before or after transplant, controls the weeds Portulaca oleracea (L., Amaranthus sp. (Mart, Sida acuta (Burm. F. and Eleusine indica (L., without affecting the growth of sugarcane cv. ‘CP52-43’ plants, compared to manual weeding.   Keywords: herbicides, Saccharum, substrate, survival

  1. Ultrastructural changes associated with cryopreservation of banana ( Musa spp.) highly proliferating meristems.

    Science.gov (United States)

    Helliot, B; Swennen, R; Poumay, Y; Frison, E; Lepoivre, P; Panis, B

    2003-03-01

    Cryopreservation has been shown to improve the frequency of virus elimination - specifically cucumber mosaic virus and banana streak virus - from banana ( Musa spp.) plants. To understand the mode of action of cryopreservation for the eradication of viral particles, we examined the ultrastructure of meristem tips at each step of the cryopreservation process. Excised meristematic clumps produced from infected banana plants belonging to cv. Williams (AAA, Cavendish subgroup) were cryopreserved through vitrification using the PVS-2 solution. We demonstrated that the cryopreservation method used only allowed survival of small areas of cells in the meristematic dome and at the base of the primordia. Cellular and subcellular changes occurring during the cryopreservation process are discussed.

  2. Qualitative and quantitative determination of protopine in Fumaria spp. by TLC-densitometry method

    Directory of Open Access Journals (Sweden)

    ATANAS PAVLOV

    2012-01-01

    Full Text Available A rapid and accurate TLC-densitometry method for qualitative and quantitative determination of protopine has been developed. The best separation was achieved using a mobile phase chloroform – ethyl acetate – methanol – ammonium hydroxide (80:80:40:0.05, v/v/v/v. The results obtained by this method (CV% 3.4 corresponded well with those obtained by using a HPLC method. The reliability of the proposed method was proved through reproducibility test with alkaloid extracts from Fumaria spp.

  3. [Research on Breeding of Dioscorea opposita cv. Tiegun].

    Science.gov (United States)

    Li, Jian-jun; Ren, Mei-ling; Wang, Jun; Jia, Guo-lun; Pei, Li-xin; Sun, Shu-wu

    2015-09-01

    To breeding the new varieties Dioscorea opposita cv. Tiegun with the best comprehensive properties. Seven new Dioscorea opposita. cv. Tiegun cultivars were screened by space mutation breeding of Dioscorea opposita cv. Tiegun bulbils. Yield,allantoin content,water soluble extractive and the resistance of these seven cultivars were compared with the main cultivar Dioscorea opposita cv. Tiegun( CK). Meanwhile, the nutrition quality of new cultivars No. 6 and No. 10 were compared with the main cultivar. (1) The fresh weight per plant ranked in the order as follows: No. 6 > No. 10 > No. 4 > No. 9 > No. 1 > CK > No. 2 > No. 8. The drying rate ranked in the order as follows: No. 2 > No. 10 > No. 9 > No. 6 > No. 8 > CK > No. 1 > No. 4. Dry weight per plant ranked in the order as follows: No. 10 > No. 9 > No. 6 > No. 2 > No. 1 > CK > No. 4 > No. 8. The fresh weight per plant, drying rate and dry weight per plant of No. 6 and No. 10 were higher than the main cultivar. (2) The allantoin content ranked in the order as follows: No. 6 > No. 4 > No. 10 > CK > No. 9 > No. 8 > No. 2 > No. 1. (3) The water soluble extractive contents ranked in the order as follows: No. 6 > No. 2 > No. 4 > No. 10 > No. 1 > CK > No. 9 > No. 8. The water soluble extractive content of No. 6 was higher than No. 10 and the main cultivar. (4) No. 10 had the best taste of dry, soft, sweet and fragrant, No. 6 had the taste of dry, floury and hard, and No. 9 had the taste of dry and crisp. (5) No. 6 had the strongest resistance to Gloeosporium pestis and Cykindrosporium dioscoreae; No. 10 had a middle resistance to Gloeosporium pestis and a strong resistance to Cykindrosporium dioscoreae; and the main cultivar had a middle degree of being prone to Gloeosporium pestis and a middle resistance to Cykindrosporium dioscoreae. (6) The content of starch, reducing sugar, protein and ash in No. 6 and No. 10 were higher than that of the main cultivar,while the content of water in No. 6 and No. 10 were lower

  4. Aeromonas spp.: an emerging pathogen?

    Directory of Open Access Journals (Sweden)

    Andrea Bartolini

    2015-12-01

    Full Text Available The aim of this study is to identify and monitor the presence of Aeromonas spp. strains in stool cultures. We analyzed 5564 stool cultures from September 2012 to August 2013. Sixty-three patients were positive for Aeromonas spp. The most frequent symptoms were: diarrhea (46.0% and abdominal pain (12.7%. Pediatric subjects were 28. Samples’ microscopic examination showed leukocytes in 38.1% of cases. It is still controversial whether Aeromonas are responsible for human gastroenteritis, but their presence in faecies of symptomatic patients supports their etiologic role. We propose search for toxins by polymerase chain reaction to identify strains that require an antibiotic therapy.

  5. Zoonotic potential of Helicobacter spp.

    Directory of Open Access Journals (Sweden)

    Irena Mladenova-Hristova

    2017-06-01

    Full Text Available The genus Helicobacter contains more than 35 species. Helicobacter pylori is the most important in terms of human health. Discovery of these helicobacters gives opportunity to understand the relationship between these bacteria which colonise the animal and human gut and their effect on the host. Infection with Helicobacter spp. and the associated diseases in their hosts allow us to study the pathogenic mechanisms. The potential zoonotic pathway for the transmission of Helicobacter spp. and epidemiology of this genus, deserve more attention to these emerging pathogens.

  6. Sarcocystis neurona and Neospora caninum in Brazilian opossums (Didelphis spp.): Molecular investigation and in vitro isolation of Sarcocystis spp.

    Science.gov (United States)

    Gondim, Leane S Q; Jesus, Rogério F; Ribeiro-Andrade, Müller; Silva, Jean C R; Siqueira, Daniel B; Marvulo, Maria F V; Aléssio, Felipe M; Mauffrey, Jean-François; Julião, Fred S; Savani, Elisa San Martin Mouriz; Soares, Rodrigo M; Gondim, Luís F P

    2017-08-30

    Sarcocystis neurona and Neospora spp. are protozoan parasites that induce neurological diseases in horses and other animal species. Opossums (Didelphis albiventris and Didelphis virginiana) are definitive hosts of S. neurona, which is the major cause of equine protozoal myeloencephalitis (EPM). Neospora caninum causes abortion in cattle and infects a wide range of animal species, while N. hughesi is known to induce neurologic disease in equids. The aims of this study were to investigate S. neurona and N. caninum in tissues from opossums in the northeastern Brazil, and to isolate Brazilian strains of Sarcocystis spp. from wild opossums for comparison with previously isolated strains. Carcasses of 39 opossums from Bahia state were available for molecular identification of Sarcocystis spp. and N. caninum in their tissues, and for sporocyst detection by intestinal scraping. In addition, Sarcocystis-like sporocysts from nine additional opossums, obtained in São Paulo state, were tested. Sarcocystis DNA was found in 16 (41%) of the 39 opossums' carcasses; N. caninum DNA was detected in tissues from three opossums. The sporocysts from the nine additional opossums from São Paulo state were tested by bioassay and induced infection in nine budgerigars, but in none of the gamma-interferon knockout mice. In vitro isolation was successful using tissues from all nine budgerigars. The isolated strains were maintained in CV-1 and Vero cells. Three of nine isolates presented contamination in cell culture and were discarded. Analysis of six isolates based on five loci showed that these parasites were genetically different from each other and also distinct from S. neurona, S. falcatula, S. lindsayi, and S. speeri. In conclusion, opossums in the studied regions were infected with N. caninum and Sarcocystis spp. and represent a potential source of infection to other animals. This is the first report of N. caninum infection in tissues from black-eared opossum (D. aurita or D

  7. Strategi Pemasaran Terumbu Karang Budidaya pada CV Bali Aquarium, Badung, Propinsi Bali

    OpenAIRE

    SURYAWARDANI, IGA OKA; PATNI, PUTU KIRANA; USTRIYANA, I NYOMAN GEDE

    2007-01-01

    The objectives of this research are to understand the marketing strategy undertaken by CV. Bali Aquarium, and to develop alternative marketing strategy that can be implemented by CV. Bali Aquarium. CV. Bali Aquarium undertakes an export business of coral. The coral production is undertaken by coral propagation technology that is located far away from the original coral habitat. The results indicate that CV. Bali Aquarium has developed marketing strategy of segmentation, targeting, pos...

  8. Enraizamento in vitro de pereira (Pyrus communis L. cv. Carrick

    Directory of Open Access Journals (Sweden)

    Erig Alan Cristiano

    2004-01-01

    Full Text Available O objetivo do presente trabalho foi avaliar o efeito do ácido naftalenoacético (ANA e do carvão ativado no enraizamento in vitro de pereira (Pyrus comunis L. cv. Carrick. Para tanto, microestacas de pereira com aproximadamente 0,8 a 1cm de comprimento foram utilizadas como explantes. Os tratamentos constituíram-se de três concentração de ANA no meio de cultura (0; 3,2 e 6,4mM e de duas concentração de carvão ativado (0 e 1%. A partir dos resultados obtidos no experimento, conclui-se que o ANA nas concentrações de 3,2 e 6,4mM e na ausência de carvão ativado no meio de cultura, possibilitou um melhor enraizamento de pereira cv. Carrick.

  9. EN-CV during LS1: upgrade, consolidation, maintenance, operation

    CERN Document Server

    Nonis, M

    2012-01-01

    The Cooling and Ventilation (CV) Group in the Engineering Department (EN) will be heavily involved in several projects and activities during the long shutdown in 2013 and 2014 (LS1) within a timeframe limited to around twelve months. According to the requests received so far, most projects are related to the upgrade of users' equipment, consolidation work, and the construction of new plants. However, through the experience gained from the first years of the LHC run, some projects are also needed to adapt the existing installations to the new operating parameters. Some of these projects are presented hereafter, outlining the impact that they will have on operational working conditions or risks of breakdown. Finally, EN-CV activities during LS1 for maintenance, operation, and commissioning will be mentioned since they represent a major workload for the Group.

  10. Workshop On Applications Payroll (Steam) CV. S & D Jaya

    OpenAIRE

    Leonita Dewanti; Sahni Damerianta, SKom, MMSI

    2003-01-01

    In a small and large companies require payroll processing is right - deadly accurate from the datas of each employee starts the way it works until the absence of a superior to subordinates sari payroll of each employee must vary to obtain accurate calculations should therefore relate to the employee services . Therefore, the payroll in the computer system is helping the financial administration to proceed in this case a "CV. S & D JAYA "need to use a computer from a manual t...

  11. STRATEGI PEMASARAN PRODUK DIGITAL PRINTING PADA CV. FNB DIGITAL JAMBI

    OpenAIRE

    Yuniarti, Yenni; Mauliana, Sarah

    2012-01-01

    CV. Digital FNB is one of the companies that involved in Digital Printing business. Based on survey in the middle of 2011-2012, there are 31 digital printing businesses in the city of Jambi. In order to examine the marketing stratey implemented, descriptive study has been conducted, uses interview, observation, and documentation as instruments. The strategy that observed in this study consist of marketing strategy including market segmentation, targeting, product strategy, pricing strategy, ...

  12. Final Environmental Assessment for the CV-22 Interim Beddown

    Science.gov (United States)

    2016-04-01

    the use of a biological simulant (i.e., Bacillus thuringiensis ), improved “housekeeping,” and periodic ground disturbance for construction of test...www.env.nm.gov/aqb/permit/documents/Public_Notice_Kirtland_AFB_ 1517M5_14Aug13. pdf . Accessed June 2015. Reese 2016 Reese, I. 2016. Personal communication...044. pdf . Accessed December 2015. USAF 2015a USAF. 2015. CV-22 Osprey Fact Sheet. Available at http://www.af.mil/DesktopModules/ArticleCS

  13. Applications in Foreign Currency Prima CV Ellindo Money Changer

    OpenAIRE

    Wisnu Sutrisno; Ire Puspa Wardhani, Skom, MMSI

    2004-01-01

    This explains the scientific writing about the design of application programs forforeign currency transactions by using Visual Basic 6.0 programming language.In scientific writing database is also used by Visual Data Manager (VisData)integrated in Visual Basic 6.0 program itself. Consists of three tables namelyCurrency,Customer and Transaction.In the end application program for foreign currency transactions will be applied to theCV. Prima Ellindo Money Changer for ease in performing activitie...

  14. Efeito das condições de manejo nas características sensoriais de banana (Musa spp. cv. Pacovan

    Directory of Open Access Journals (Sweden)

    Maria Sueli Francisco

    2014-06-01

    Full Text Available Estudou-se o efeito de quatro condições de manejo sobre a aceitação de banana 'Pacovan'. Os frutos foram colhidos no ponto de maturação fisiológica ideal para o armazenamento, em quatro propriedades rurais do município de Bananeiras - PB, seguindo-se delineamento inteiramente casualizado. Os tratamentos foram identificados como: P1: irrigação+adubação química e orgânica +controle químico de plantas invasoras; P2: controle manual de plantas invasoras; P3: irrigação+adubação química+controle manual de plantas invasoras; P4: controle manual e químico de plantas invasoras. Em cada propriedade, foram colhidos cinco cachos de banana que foram submetidos à climatização com carbureto de cálcio por 12 horas para uniformização da maturação. Após esse período, foram mantidos à temperatura ambiente até sua completa maturação. O painel sensorial foi composto por 62 julgadores não treinados, com idade média de 18 anos. Os atributos aroma, sabor, textura e cor da polpa foram avaliados por meio de teste de aceitação, utilizando-se de escala hedônica estruturada de 9 pontos. Para o teste de intenção de compra, foi utilizada escala estruturada de 5 pontos. As amostras foram apresentadas aos julgadores na forma de rodelas, com espessura aproximada de 1,5 cm. Os resultados foram submetidos à análise de variância (ANOVA, e as médias, comparadas pelo teste de Tukey, ao nível de 5% de probabilidade. Para todos os atributos avaliados,os valores situaram-se entre 7,34 e 7,68, posicionando-se na escala hedônica entre os termos 'gostei moderadamente' e 'gostei muito'. Para intenção de compra, o percentual de aceitação foi de 74,19; 72,58; 72,58 e 70,97%, respectivamente, para as amostras P1, P2, P3 e P4. O tipo de manejo não influenciou na aceitação da banana 'Pacovan', sendo os frutos considerados pelos julgadores como produto de boa aceitação.

  15. Genome-wide analysis of transcription factors during somatic embryogenesis in banana (Musa spp.) cv. Grand Naine.

    Science.gov (United States)

    Shivani; Awasthi, Praveen; Sharma, Vikrant; Kaur, Navjot; Kaur, Navneet; Pandey, Pankaj; Tiwari, Siddharth

    2017-01-01

    Transcription factors BABY BOOM (BBM), WUSCHEL (WUS), BSD, LEAFY COTYLEDON (LEC), LEAFY COTYLEDON LIKE (LIL), VIVIPAROUS1 (VP1), CUP SHAPED COTYLEDONS (CUC), BOLITA (BOL), and AGAMOUS LIKE (AGL) play a crucial role in somatic embryogenesis. In this study, we identified eighteen genes of these nine transcription factors families from the banana genome database. All genes were analyzed for their structural features, subcellular, and chromosomal localization. Protein sequence analysis indicated the presence of characteristic conserved domains in these transcription factors. Phylogenetic analysis revealed close evolutionary relationship among most transcription factors of various monocots. The expression patterns of eighteen genes in embryogenic callus containing somatic embryos (precisely isolated by Laser Capture Microdissection), non-embryogenic callus, and cell suspension cultures of banana cultivar Grand Naine were analyzed. The application of 2, 4-dichlorophenoxyacetic acid (2, 4-D) in the callus induction medium enhanced the expression of MaBBM1, MaBBM2, MaWUS2, and MaVP1 in the embryogenic callus. It suggested 2, 4-D acts as an inducer for the expression of these genes. The higher expression of MaBBM2 and MaWUS2 in embryogenic cell suspension (ECS) as compared to non-embryogenic cells suspension (NECS), suggested that these genes may play a crucial role in banana somatic embryogenesis. MaVP1 showed higher expression in both ECS and NECS, whereas MaLEC2 expression was significantly higher in NECS. It suggests that MaLEC2 has a role in the development of non-embryogenic cells. We postulate that MaBBM2 and MaWUS2 can be served as promising molecular markers for the embryogencity in banana.

  16. The draft genome of Corchorus olitorius cv. JRO-524 (Navin

    Directory of Open Access Journals (Sweden)

    Debabrata Sarkar

    2017-06-01

    Full Text Available Here, we present the draft genome (377.3 Mbp of Corchorus olitorious cv. JRO-524 (Navin, which is a leading dark jute variety developed from a cross between African (cv. Sudan Green and indigenous (cv. JRO-632 types. We predicted from the draft genome a total of 57,087 protein-coding genes with annotated functions. We identified a large number of 1765 disease resistance-like and defense response genes in the jute genome. The annotated genes showed the highest sequence similarities with that of Theobroma cacao followed by Gossypium raimondii. Seven chromosome-scale genetically anchored pseudomolecules were constructed with a total size of 8.53 Mbp and used for synteny analyses with the cocoa and cotton genomes. Like other plant species, gypsy and copia retrotransposons were the most abundant classes of repeat elements in jute. The raw data of our study are available in SRA database of NCBI with accession number SRX1506532. The genome sequence has been deposited at DDBJ/EMBL/GenBank under the accession LLWS00000000, and the version described in this paper will be the first version (LLWS01000000.

  17. Differentiation of Phytopathogenic agrobacterium spp.

    OpenAIRE

    Kuzmanović, Nemanja; Ivanović, Milan; Ćalić, Anđelka; Gašić, Katarina; Obradović, Aleksa

    2011-01-01

    Due to the difficulties in differentiation of phytopathogenic Agrobacterium spp. and lack of a standardized protocol, we carried out selection and evaluation of suitable methods based on the bacterial physiological, genetic and pathogenic properties. Strains of Agrobacterium tumefaciens, A. rhizogenes and A. vitis were differentiated using standard bacteriological and molecular methods. The biochemical and physiological tests confirmed authenticity of the s...

  18. Morphological and molecular characterization of Fusarium spp. associated with olive trees dieback in Tunisia.

    Science.gov (United States)

    Trabelsi, Rahma; Sellami, Hanen; Gharbi, Yâakoub; Krid, Samira; Cheffi, Manel; Kammoun, Sonia; Dammak, Mariem; Mseddi, Aymen; Gdoura, Radhouane; Triki, Mohamed Ali

    2017-05-01

    Dieback and wilting symptoms caused by complex soilborne fungi are nowadays the most serious threatening disease affecting olive trees (Olea europaea) in Tunisia and presumably in many Mediterranean basin countries. Fusarium is one of the important phytopathogenic genera associated with dieback symptoms of olive trees. The objective of the present study was to confirm the pathogenicity of Fusarium spp. isolated from several olive-growing areas in Tunisia. According to the pathogenic test done on young olive trees (cv. Chemlali), 23 out of 104 isolates of Fusarium spp. were found to be pathogenic and the others were weakly or not pathogenic. The pathogenic Fusarium spp. isolates were characterized using molecular methods based on ITS PCR. Isolation results revealed the predominance of Fusarium solani (56.5%) and F. oxysporum species (21.7%) compared to F. chalmydosporum (8.7%), F. brachygibbosum (8.7%) and F. acuminatum (4.34%). Based on pathogenicity test, disease severity was highly variable among the 23 pathogenic isolates tested (P Fusarium spp. might be a major agent causing dieback disease of olive trees in Tunisia.

  19. Capacidad de recuperación de 23 accesiones de Leucaena spp. después de la poda Recovery capacity of 23 Leucaena spp. accessions after pruning

    Directory of Open Access Journals (Sweden)

    Hilda B Wencomo

    2011-03-01

    Full Text Available Se evaluaron 23 accesiones de Leucaena spp. durante dos años, con el objetivo de conocer la variabilidad existente en la población en cuanto a la capacidad de recuperación después de la poda. Cuando las plantas sobrepasaban los 2 m, se realizó la poda de uniformidad a un 1 m de altura sobre el nivel del suelo. Las mediciones fueron: el grosor del tallo, el número de rebrotes y su longitud con una frecuencia semanal, a partir de lo cual se calculó la velocidad de crecimiento de cada accesión; además se determinó el rendimiento. El análisis de componentes principales, el de conglomerados y el de regresión lineal simple se realizaron a través del programa estadístico SPSS versión 11.5 para Microsoft Windows®. Se observó variabilidad en los indicadores evaluados (86,11% acumulado en la componente y todos contribuyeron a su explicación. A su vez, el análisis de conglomerados permitió la formación de tres grupos. Se concluye que todas las accesiones presentaron capacidad de recuperación después de la poda, con diferencias entre ellas. Las accesiones Leucaena leucocephala cv. Cunningham, cv. Perú, CIAT-9119, CIAT-9438, CIAT-751, CIAT-7988, CIAT-7384, CIAT-7929, CIAT-17480, cv. Ipil-Ipil y cv. CNIA-250; L. lanceolata CIAT-17255 y CIAT-17501 y L. diversifolia CIAT-17270 fueron las de mayor capacidad de recuperación. Por ello se recomienda, en futuras investigaciones relacionadas con la caracterización, evaluación y selección de estas especies, realizar estudios que incluyan su persistencia en el tiempo bajo condiciones de poda, así como su empleo en el fomento de nuevos sistemas silvopastoriles.Twenty three Leucaena spp. accessions were evaluated for two years, in order to know the variability existing in the population regarding the recovery capacity after pruning. When the plants were higher than 2 m, the uniformity pruning was made at 1 m above the soil level. The measurements were: stem diameter, number of regrowths and

  20. Analisis Daya Saing untuk Meningkatkan Kualitas Layanan terhadap Pelanggan dengan Pendekatan CRM di CV. BLITZSPOT

    Directory of Open Access Journals (Sweden)

    Suryadi Muhammad

    2017-04-01

    Full Text Available CV. Blitzspot adalah perusahaan yang bergerak di bidang jasa penyediaan layanan internet (internet service provider, yang berkantor pusat di Komplek Padasuka Indah Ruko Blok A-1, Cimahi, Jawa Barat. Berdasarkan hasil wawancara dan hasil observasi pada data transaksi CV. Blitzspot, konsumen CV. Blitzspot yang melakukan transaksi tiap tahunnya selalu berkurang, hal ini dikarenakan konsumen sering membandingkan layanan-layanan yang ditawarkan oleh para pesaing CV. Blitzspot. Bila permasalahan tersebut belum teratasi, konsumen CV. Blitzspot akan menurun tiap tahunnya dan akan selalu berpindah ke pesaing CV. Blitzspot. Direktur Utama CV. Blitzspot ingin menganalisis daya saing untuk menentukan layanan-layanan yang akan diberikan kepada pelanggan CV. Blitzspot dan penentuan strategi untuk mempertahankan konsumen CV. Blitzspot.  Metode Analisis daya saing yang digunakan adalah Model Analisis Berlian Porter dan Metode Pengelompokkan Pelanggan yang digunakan adalah Metode LRFM. Berdasarkan hasil pengujian dan pembahasan yang telah dibuat, maka dapat ditarik kesimpulan bahwa Direktur Utama dapat menganalisis daya saing CV. Blitzspot dengan menggunakan Berlian Porter untuk meningkatkan kualitas layanan terhadap pelanggan CV. Blitzspot.

  1. Effect of Sodium Cyanide on Wheat (Triticum durum cv. Altar and T. aestivum cv. Cumhuriyet)

    DEFF Research Database (Denmark)

    Gemici, Meliha; Karshenass, A.M.; Tan, Kit

    2008-01-01

    The effect of sodium cyanide on the morphology of stem, leaves and grain yields of Triticum durum cv. Altar and Triticum aestivum cv. Cumhuriyet grown under glass was studied. Seeds were planted in six different sets of pots containing ordinary garden soil. After formation of the first leaves...

  2. Induction of single flower mutant in Hibiscus cv. 'Alipur Beauty'

    International Nuclear Information System (INIS)

    Banerji, B.K.; Datta, S.K.

    1986-01-01

    Stem cuttings of Hibicus cv. 'Alipur Beauty' were irradiated with 0, 1.0, 2.0 Krad of gamma rays in 60 Co source and planted just after irradiation. Data were recorded on days to sprout, number of sprouts, sprouting percentage, survival, plant height, leaf number and size, abnormal leaves and plants, floral abnormalities and induction of somatic mutations in flower colour/form. Some of the characters like plant height, leaf number, abnormal leaves and plants and leaf size were recorded after 60 and 120 days. 7 refs. (author)

  3. Odour-active compounds in banana fruit cv. Giant Cavendish.

    Science.gov (United States)

    Pino, Jorge A; Febles, Yanet

    2013-11-15

    Application of solid-phase microextraction, simultaneous distillation-extraction and liquid-liquid extraction, combined with GC-FID, GC-MS, aroma extract dilution analysis, and odour activity value were used to analyse volatile compounds from banana fruit cv. Giant Cavendish and to estimate the most odour-active compounds. The analyses led to the identification of 146 compounds, 124 of them were positively identified. Thirty-one odourants were considered as odour-active compounds and contribute to the typical banana aroma, eleven of them are reported for the first time as odour-active compounds. Copyright © 2013 Elsevier Ltd. All rights reserved.

  4. OpenCV 3.0 computer vision with Java

    CERN Document Server

    Baggio, Daniel Lélis

    2015-01-01

    If you are a Java developer, student, researcher, or hobbyist wanting to create computer vision applications in Java then this book is for you. If you are an experienced C/C++ developer who is used to working with OpenCV, you will also find this book very useful for migrating your applications to Java. All you need is basic knowledge of Java, with no prior understanding of computer vision required, as this book will give you clear explanations and examples of the basics.

  5. Analisa Risiko Proyek Pengembangan Software Pada CV. XYZ

    OpenAIRE

    Apriatono, Nicolas Adriaan; Wibowo, Adi; Gunawan, Ibnu

    2015-01-01

    CV.XYZ is a small size company that works on software engineering.The worker in this company is not many, between 5 – 8 people only. The problem in this company is there are no identification of risks that can happen. The examples of the problems are the clients suddenly request some features for their software, no milestone on the project, no data about clients' system and no monitoring on the ongoing projects. These things can hold back the company's performance. Thus, risk analysis is need...

  6. The phenolic extractives in southern red oak (Quercus falcata Michx. var. falcata)

    Science.gov (United States)

    Seiji Ohara; Richard W. Hemingway

    1989-01-01

    The bark of southern red oak (Quercus falcala Michx. var. falcala) is a rich source of quercitrin (quercetin-3-rhamnoside). It contains only low concentrations of (+)-catechin and no significant amounts of epicatechin or gallocatechin. The three major dimeric proanthocyanidins present are epicatechin-(4β→8)-...

  7. Induced mutations in Iraqi bread wheat cv. Saber Beg

    International Nuclear Information System (INIS)

    Ibrahim, I.F.; Haidar, H.O.

    1989-01-01

    ''Saber Beg'', is a local wheat cultivar important in the semi-arid zone of Iraq where the rainfall is less than 450 mm per year. This cultivar has a good baking quality, but is of low productivity, high susceptibility to common bunt (Tilletia spp.) and to leaf rust (Puccinia recondita Rob. ex Desm.), but only in the rainy season. A mutation breeding programme using gamma irradiation has been started in 1978 to improve this cultivar. Seeds of all main tillers from M 1 plants were harvested and artificially inoculated with teliospores of Tilletia spp. All the seeds from healthy M 2 plants were inoculated again and sown in the same area. Out of 22920 M 3 plants, 244 resistant ones were selected. During subsequent screening for 4 generations, however, only 3 mutants were confirmed

  8. Assessment of Genetic Diversity in Opuntia spp. Portuguese Populations Using SSR Molecular Markers

    Directory of Open Access Journals (Sweden)

    Carlos M. G. Reis

    2018-04-01

    Full Text Available The Opuntia spp., most likely few individuals, were introduced in the Iberian Peninsula in the beginning of the 16th century, after the discovery of America, spreading afterwards throughout the Mediterranean basin. We analysed, for the first time, the genetic diversity in a set of 19 Portuguese Opuntia spp. populations from the species O. ficus-indica, O. elata, O. dillenii and O. robusta using nuclear microsatellite (nuSSR markers. The Italian cultivars ‘Bianca’, ‘Gialla’ and ‘Rossa’ were included in the study for comparison purposes. The nuSSR amplifications produced from five to 16 alleles, with an average of 9.2 alleles per primer pair, and average polymorphism information content of 0.71. The estimated Dice coefficient among populations varied from 0.26 to 1.0, indicating high interspecific genetic diversity but low genetic diversity at the intraspecific level. The hierarchical clustering analysis revealed four major groups that clearly separated the four Opuntia species. Among the O. ficus-indica populations, two sub-clusters were found, one including the white pulp fruits (with cv. Bianca and the other with the orange pulp ones and including the cv. Gialla, the cv. Rossa, and one pale yellow pulp population. No genetic differences were found between the inermis form, O. ficus-indica f. ficus-indica, and the rewilded spiny one, O. ficus-indica f. amyclaea. The dendrogram indicated that the clustering pattern was unrelated to geographical origin. The results revealed a low level of genetic diversity among the Portuguese populations of O. ficus-indica.

  9. Perancangan Kemasan Transportasi Buah Jambu Air (Syzygium aqueum cv Camplong

    Directory of Open Access Journals (Sweden)

    Iswahyudi

    2015-04-01

    Full Text Available Jamboo cv Camplong was an exotic fruit from Sampang Indonesia which had high economic values. The quality of fresh Jamboo was greatly influenced by the types of packaging and ways of its transportation that affected its shelf life. The purpose of this research were designing a primary packaging and analyzing the quality of Jamboo cv Camplong after short transportation (from Sampang to Surabaya. Farmers used conventional packaging with capacity of 8.4 kg/box (dimension 478 mm x 146 mm x 354 mm. Based on theoretical packaging design showed that the flute BC cardboard (capacity 4.5 kg/box, dimension 357 mm x 217 mm x 216 mm with partition flute A cardboard. In fact, there was needed modification of the dimension (342 mm x 210 mm x 200 mm because of the fruits diameter (60-65 mm. This result did not change the efficiency usage of transportation space (91-95% and compression strength of box can support (7 boxes/ stack. The result showed that the mechanical damage after transportation were 20.87% for conventional packaging (as control and 7.70% for modification packaging design (packaging with partition.

  10. Resistance selection on banana CV. Ambon Kuning Against Fusarium Wilt

    International Nuclear Information System (INIS)

    Sutarto, Ismiyarti; Meldia, Yeni; Jumjunidang

    1998-01-01

    This research was conducted in order to study the occurrence of mutation on irradiated plantlets and their resistance of plants of banana cv. Ambon Kuning against Fusarium wilt. Plantlets of banana cv. Ambon Kuning sized 5 cm were exposed to gamma rays at the doses 5 - 35 Gy intervals, then were subcultured for obtained M 1 V 5 plantlets. More over, the planlets were acclimatized and were planted in the field was already infected by Fasarium (f).culbense (FOC). The result indicated that irradiated plantlets of the doses 20 - 35 Gy were not able to survive up to 6 months after exposing to gamma rays. Abnormalities of M 1 V 5 plantlets originated from irradiated plantlets at the doses 10 and 15 Gy were shown on rossette plantlets with rigid and dark green leaves, and the formation of smooth mass morphologically shaped like calculus. The appearance of plant height and number of suckers of suckers of M 1 V 5 plants in the field was quite various. The number of survival plants after 8 moths planting was 8, 7, 15, and 28, respectively originated from untreated plants and irradiated plantlets at the doses 5, 10, and 15 Gy. After one year planting , only 2 plants were able to survive from irradiated plantlet at the dose 15 Gy. The plants could produce 27 plantlets obtained from culturing their shoot tips. Further study of these plantlets was needed in order create the stability of their resistance to FOC. (author)

  11. Flowering induction of Hemerocallis hybrida cv. Graziela Barroso by gibberellic acid aplication / Indução do florescimento de Hemerocallis hybrida cv. Graziela Barroso pela aplicação de ácido giberélico

    Directory of Open Access Journals (Sweden)

    Nilce Nazareno da Fonte

    2009-12-01

    Full Text Available Hemerocallis spp. production is important in Santa Catarina State. The flowers have a great use in landscaping, and bloom in spring. This work had the aim of studying the flower induction in Hemerocallis hybrida cv Graziela Barroso by different applications of gibberellic acid (0 mgL-1 control; 15 mgL-1; 30 mgL-1 GA3 during summer, autumn and winter. A completely randomized design was used. Treatments represented a factorial arrangement of three GA3 concentrations and ten application occasions in each season. Interaction between the factors was evaluated by the following variables: dry matter (summer - the best value found was 13,81 g (in 30 mgL-1 GA3 in the April, 16 application, and foliar area (autumn - 436,3 cm2 (in 30 mgL-1 GA3 the July, 1st application. The gibberellic acid cannot be indicated to increase the commercial qualities (blooming and growing of Hemerocallis hybrida cv Graziela Barroso. A produção de Hemerocallis spp., planta muito utilizada no paisagismo, que floresce naturalmente na primavera, tem destaque em Santa Catarina. O objetivo do presente trabalho foi estudar a indução do florescimento em Hemerocallis hybrida cultivar Graziela Barroso, pela aplicação ácido giberélico (0 mgL-1 controle; 15 mgL-1; 30 mgL-1 de GA3, no verão (janeiro-abril, outono (abril-julho e inverno (julho-outubro, de forma a aprimorar seus atributos comerciais. Foi utilizado um delineamento experimental inteiramente casualizado, com fatorial 3x10 (três doses de GA3 e dez datas de aplicação para cada estação. Houve interação entre os fatores estudados apenas para acúmulo de massa seca (verão, sendo o melhor valor encontrado 13,81 g (30 mgL-1 de GA3, na aplicação de 16 de abril; e área foliar (outono, 436,3 cm2 (30 mg L-1 de GA3 na aplicação de 1 de julho. Não se pode indicar o GA3 nas concentrações estudadas para aprimorar atributos comerciais (florescimento e crescimento na espécie em questão.

  12. Wilting and biological additive effect on in situ degradability and chemical composition of Arachis pintoi cv Belomonte silage

    OpenAIRE

    Rosana Aparecida Possenti; Evaldo Ferrari Júnior; Valdinei Tadeu Paulino; Ivani Pozar Otsuk; Patrícia Brás

    2010-01-01

    The purpose of this work was to evaluate the effect of wilting and biological additive amendment on chemical composition, fermentation and ruminal degradability of Arachis pintoi cv Belmonte silage. The following treatments were analysed: T1- Arachis pintoi cv Belmonte fresh forage; T2 - Arachis pintoi cv Belmonte fresh forage plus bacterial additive added to the forage prior to the ensilage; T3- Arachis pintoi cv Belmonte wilted by the sun for 4 hours; T4- Arachis pintoi cv Belmonte wilted b...

  13. Effect of fertilization on yields and fodder value of winter pea cv. Maksimirski ozimi in wheat cv. Sana mixture

    Directory of Open Access Journals (Sweden)

    Darko Uher

    2006-12-01

    Full Text Available Two year field trials (1999-2001 were carried out to determine the effect of seed winter pea inoculation and nitrogen top-dressing effect on nodule dry weight of pea root and also on the dry matter yield and fodder value of winter pea cv. Maksimirski ozimi and wheat cv. Sana mixture. Just before sowing the inoculation of pea seeds was performed by the indigenous variety of Rhizobium leguminosarum bv. viciae which is part of the microbial collection of the Department of Microbiology at the Faculty of Agriculture University of Zagreb. The highest total nodule dry weight on pea root was determined on the inoculated variant 2 (0.710 g/variant. Total dry matter yields were ranging from 8.64 t ha-1 (control up to 10.98 t ha-1 (inoculation. Yields crude proteins pea in 2001 were ranging from 942 kg ha-1 (control up to 1510 kg ha-1 (inoculation and for wheat, those values were from 450 kg ha-1 (control up to 813 kg ha-1 (nitrogen top-dressing. Total crude proteins mixture yields were from 1392 kg ha-1 (control up to 2140 kg ha-1 (inoculation.

  14. Effect of fertilization on yields and fodder value of winter pea cv. Maksimirski ozimi in triticale cv. Clercal mixture

    Directory of Open Access Journals (Sweden)

    Darko Uher

    2007-10-01

    Full Text Available Two year field trials (1999 - 2001 were performed to determine theinoculation seed winter pea and nitrogen top-dressing effect on nodule dry weight of pea root and also on the dry matter yield of winter pea cv. Maksimirski ozimi and triticale cv. Clercal mixture. Immediately before sowing the inoculation of pea seeds was performed by the indigenous variety of Rhizobium leguminosarum bv. viciae which is part of Department of Microbiology collection at the Faculty of Agriculture University of Zagreb. The highest total nodule dry weight on pea root was determined on the inoculated variant 2 (0,635 g / variant. Total dry matter yields were ranging from 9,10 t ha-1 (control up to 12,06 t ha-1 (nitrogen top-dressing. Crude proteins pea yields in 2001 were ranging from 798 kg ha-1 (nitrogen topdressing + inoculation up to 1087 kg ha-1 (inoculation. For triticale, those values were from 568 kg ha-1 (control up to 957 kg ha-1 (nitrogen topdressing. Total crude proteins mixture yield ranged from 1414 kg ha-1 (control up to 1808 kg ha-1 (nitrogen top-dressing.

  15. Cloning and sequencing of columbid circovirus (coCV), a new circovirus from pigeons.

    Science.gov (United States)

    Mankertz, A; Hattermann, K; Ehlers, B; Soike, D

    2000-01-01

    The complete nucleotide sequence of columbid circovirus (CoCV) isolated from pigeons is described. CoCV was amplified using a consensus primer PCR approach directed against conserved sequences within the rep genes of vertebrate circoviruses. The genome of CoCV is circular and 2037 nt in size. It displays 55% homology to the genome of psittacine beak and feather disease virus and is more distantly related (circo-, nano- and geminiviruses. Phylogenetic analyses suggest classification of CoCV as member of the genus Circovirus of the virus family Circoviridae.

  16. Pengaruh Budaya Organisasi, Kepemimpinan, dan Motivasi Kerja terhadap Kepuasan Kerja Karyawan CV Surya Raya

    Directory of Open Access Journals (Sweden)

    Laksmi Sito Dwi Irvianti

    2012-05-01

    Full Text Available This research was conducted on CV Surya Raya which is engaged in car shipping. The aim of this study is to determine the influence of organization culture, leadership, and working motivation partially to employees’ satisfaction in CV Surya Raya. The data was collected by conducting interviews with the company and distributing questionnaires to 67 employees as respondents and then analyzed using linear regression analysis method. From the research results, leadership variable does not have a significant impact on employees' job satisfaction in CV Surya Raya. Whereas, the motivation variable has greater influence on job satisfaction of CV Surya Raya employees compared to the organization's culture.

  17. CV activities on the LHC complex during the long shutdown

    CERN Document Server

    Deleval, S; Body, Y; Obrecht, M; Moccia, S; Peon, G

    2011-01-01

    The presentation gives an overview of the major projects and work foreseen to be performed during next long shutdown on cooling and ventilation plants. Several projects are needed following the experience of the last years when LHC was running, in particular the modifications in the water cooling circuits presently in overflow. Some other projects are linked to the CV consolidation plan. Finally, most of the work shall be done to respond to additional requests: SR buildings air conditioning, the need to be able to clean and maintain the LHC cooling towers without a complete stop of cooling circuits, the upgrade of the air conditioning of the CCC rack room cooling etc. For all these activities, the author will detail constraints and the impact on the schedule and on the operation of the plants that will however need to run for most of the shutdown duration. The consequence of postponing the long shutdown from 2012 to 2013 will be also covered.

  18. Gamma irradiation studies on Gladiolus cv. White Friendship

    International Nuclear Information System (INIS)

    Banerji, B.K.; Datta, S.K.; Sharma, S.C.

    1994-01-01

    Dormant corms of Gladiolus cv. White Friendship were irradiated with 250, 500, 750, 1000 and 1250 Gy of gamma rays. Reduction in survival, plant height, number of leaves and floret, spike length, leaf and corm size and delayed flowering were recorded after irradiation. Morphological abnormalities in foliage and florets and chromosomal aberration during root tip mitosis increased with increase in exposure. Flowering was ceased cent per cent in highest dose of 1250 Gy. LD 50 on survival basis was found in 750 Gy exposure. MV 2 and MV 3 also followed the similar pattern of results as exhibited by MV 1 plants. Pink flower colour mutation was detected in few plants as sectorial chimeric form in MV 2 in treated population. In MV 3 one plant produced spike with lighter pink florets in 750 Gy treatment. This mutant has been isolated in pure form. (author). 10 refs., 2 figs., 2 tabs

  19. Propolis extract in postharvest conservation Solo papaya cv. 'Golden'

    Directory of Open Access Journals (Sweden)

    Flávia Regina Passos

    2016-12-01

    Full Text Available The high perishability of papaya (Carica papaya L. reduces its lifespan as well as limits marketing. Coating the fruits is an alternative to aid food preservation. We aimed to evaluate the effects of coating propolis extract on the physicochemical characteristics of papaya stored at room temperature. Solo papayas cv. 'Golden' were randomly divided into five postharvest treatment groups, three forms of dip-coating (70% alcohol, hydroalcoholic extract of propolis to 2.5%, and hydroalcoholic extract of propolis to 5% and two controls (one uncoated and one with refrigerated uncoated fruits. The weight loss, firmness, soluble solids (SS, titratable acidity (TA, SS/TA, and hydrogen potential (pH were evaluated at the intervals of 4 days every 12th storage day. Sensory analysis was performed on the 4th day of storage of papayas and evaluated by untrained through the acceptance testing. Treatments “refrigerated”, “propolis 2.5%”, and “5% propolis” presented with the lowest weight loss. The firmness level for treatment propolis 5% was superior to that of control, alcohol, and 2.5% propolis treatments. The SS was greater for propolis 5% treatment, which only differed from the alcohol treatment. The TA and SS/TA did not vary with the treatments, but with the storage time. The pH of refrigerated papaya showed significant differences in relation to other treatments. The fruits subjected to refrigerated treatment presented with chilling injury. Papayas coated with propolis extract showed sensory acceptability similar to that in other treatments on the 4th day of storage. The coating of propolis extract is a promising alternative for the control of weight loss and for the maintenance of firmness in Solo papaya cv. 'Golden'.

  20. African Journal of Biotechnology - Vol 8, No 10 (2009)

    African Journals Online (AJOL)

    Using microsatellite (SSR) and morphological markers to assess the genetic diversity of 12 falcata (Medicago sativa spp. falcata) populations from Eurasia · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT DOWNLOAD FULL TEXT ... DOWNLOAD FULL TEXT. HW Karuri, EM Ateka, R Amata, AB Nyende, AWT Muigai ...

  1. In vitro organogenesis of zucchini squash cv. Caserta Organogênese in vitro de abobrinha-de-moita cv. Caserta

    Directory of Open Access Journals (Sweden)

    Liliane Cristina L Stipp

    2012-06-01

    Full Text Available A protocol for the in vitro culture of Cucurbita pepo cv. Caserta was studied, using a cotyledon segment with an attached hypocotyl fragment as an explant. First, to determine the optimal seedling age, explants were collected from 4 to 6-day-old in vitro germinated seedlings and cultured in MS basal medium supplemented with benzylaminopurine (BAP, 4.5 µM, under a 16-h photoperiod at 27ºC. Based on the results obtained, the explants collected from the 4-day-old seedlings were then cultured in MS basal medium supplemented with different concentrations of BAP (0, 1.1, 2.2, 3.3, 4.5, or 5.5 µM and incubated under a 16-h photoperiod at 27ºC. In vitro organogenesis was most efficient with explants collected from 4-day-old seedlings cultured in medium supplemented with 4.5 µM of BAP. After 4 weeks of incubation the development of adventitious buds at the cotyledon/hypocotyl junction could be observed. These buds were transferred to elongation and rooting medium and the developed plants were acclimatized to greenhouse conditions. The morphogenic process was characterized using light and scanning electron microscopy analyses to confirm the organogenesis. The results showed that this alternate explant is efficient for in vitro culture of zucchini squash cv. Caserta. The protocol will be further examined for future use in genetic transformation experiments in this species.O objetivo do trabalho foi estudar um protocolo para o cultivo in vitro de Cucurbita pepo cv. Caserta utilizando como explante um segmento de cotilédone associado a um fragmento do hipocótilo. Explantes foram coletados de plântulas germinadas in vitro com 4 a 6 dias de idade, cultivados em meio de cultura basal MS suplementado com benzilaminopurina (BAP, 4,5 µM, e incubados sob fotoperíodo de 16 h, a 27ºC. Com base nos resultados obtidos, explantes coletados de plântulas germinadas in vitro com 4 dias de idade foram cultivados em meio de cultura MS, suplementados com

  2. Ryegrass cv. Lema and guava cv. Paluma biomonitoring suitability for estimating nutritional contamination risks under seasonal climate in Southeastern Brazil.

    Science.gov (United States)

    Bulbovas, Patricia; Camargo, Carla Z S; Domingos, Marisa

    2015-08-01

    The risks posed by nutrient deposition due to air pollution on ecosystems and their respective services to human beings can be appropriately estimated by bioindicator plants when they are well acclimated to the study region environmental conditions. This assumption encouraged us to comparatively evaluate the accumulation potential of ryegrass cv. Lema and guava cv. Paluma macro and micronutrients. We also indicated the most appropriate species for biomonitoring nutrient contamination risks in tropical areas of Southeastern Brazil, which are characterized by marked dry and wet seasons and complex mixtures of air pollutants from different sources (industries, vehicle traffic and agriculture). The study was conducted in 14 sites with different neighboring land uses, within the Metropolitan Region of Campinas, central-eastern region of São Paulo State. The exposure experiments with ryegrass and guava were consecutively repeated 40 (28 days each) and 12 (84 days each) times, respectively, from Oct/2010 to Sept/2013. Macro and micronutrients were analyzed and background concentrations and enrichment ratios (ER) were estimated to classify the contamination risk within the study region. Significantly higher ER suggested that ryegrass were the most appropriate accumulator species for N, S, Mg, Fe, Mn, Cu and Zn deposition and guava for K, Ca, P and B deposition. Based on these biomonitoring adjustments, we concluded that the nutrient deposition was spatially homogeneous in the study area, but clear seasonality in the contamination risk by nutritional inputs was evidenced. Significantly higher contamination risk by S, Fe, K and B occurred during the dry season and enhanced contamination risk by Mn, Cu and Zn were highlighted during the wet season. Distinctly high contamination risk was estimated for S, Fe and Mn in several exposure experiments. Copyright © 2015 Elsevier Inc. All rights reserved.

  3. Death of pastures syndrome: tissue changes in Urochloa hybrida cv. Mulato II and Urochloa brizantha cv. Marandu

    Directory of Open Access Journals (Sweden)

    N. G. Ribeiro-Júnior

    Full Text Available Abstract The quality of forage production is a prerequisite to raising livestock. Therefore, income losses in this activity, primarily cattle raising, can result in the impossibility of economic activity. Through the qualitative and quantitative anatomical study of Urochloa hybrida cv. Mulato II and U. brizantha cv. Marandu, we searched for descriptions and compared changes in the individual vegetative body from populations with death syndrome pastures (DPS. Specimens were collected at different physiological stages from farms in northern Mato Grosso. After collection, the individuals were fixed in FAA50 and stored in 70% alcohol. Histological slides were prepared from the middle third of the sections of roots, rhizomes, and leaves, and the proportions and characteristics of tissues were evaluated in healthy, intermediate, and advanced stages of DPS. Changes were compared between cultivars. With the advancement of the syndrome, the following changes were observed: a more marked decrease in the length of roots in U. hybrida; disorganization of the cortical region of the roots and rhizome cultivars; fungal hyphae in roots and aerenchyma formation in U. hybrida; a decrease in sclerenchyma fiber proportions in roots and leaves; sclerification of the epidermis of U. brizantha rhizomes; and an increase in pericyclic fibers in U. hybrida. Furthermore, there was a decrease in the volume of epidermal cells of the abaxial face of the leaves of both cultivars, with a greater reduction in U. hybrida; a gradual decrease in thickness in the midrib of leaves similar to leaf mesophyll; conduction system obstructions; partial or total cell lysis in roots and rhizomes affected by the syndrome. Obstructions in sieve tube element and companion cells, and sometimes obstruction in xylem vessel elements. The evolution of DPS in cultivars was similar, but there were variations, arising probably from the physiological response to stress, such as aerenchyma formation in

  4. CV-Online tahab Londoni börsile / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Personaliotsinguga tegelev firma tahab tulevikus minna Londoni ja OMXi börsile. Diagramm: CV-Online Group loodab tänavu esmakordselt positiivse kulumieelse ärikasumi. Vt. samas: Üks CV-Online'i aktsionäridest on ka Google'i aktsionär; Väsinud ootamast esimest IPOt

  5. PERUMUSAN STRATEGI BERSAING JAHE INSTAN PRODUK CV. INTRAFOOD SURAKARTA MENGGUNAKAN PERCEPTUAL MAPPING

    Directory of Open Access Journals (Sweden)

    Mohd. Harisudin

    2013-11-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui posisi bersaing jahe instan produk CV. Intrafood dan strategi bersaing yang dapat direkomendasikan untuk meraih keberasilannya. Metode dasar yang digunakan adalah analisis deskriptif. Penentuan lokasi penelitian ditentukan dengan metode purposive, yaitu CV. Intrafood Surakarta. Jenis data yang digunakan dalam penelitian ini adalah data primer dan data sekunder. Metode analisis data menggunakan analisis Perceptual Mapping. Dari hasil penelitian diperoleh informasi bahwa jahe instan produk CV Intrafood berada pada peringkat ke-2 dari produk empat jahe instan yang diperbandingkan. Atribut yang dapat dijadikan kekuatan utama dalam meningkatkan pemasaran jahe instan produk CV. Intrafood adalah manfaat produk. Sisi yang paling lemah adalah atribut desain kemasan dan kinerja produk dalam kemasan. Abstract This study aims to determine the competitive position of the product instant ginger CV. Intrafood and competitive strategy can be recommended to achieve success. The basic method used is descriptive analysis. Determining the location of the study are determined by purposive method, namely CV. Intrafood Surakarta. Data used in this study is primary data and secondary data. Method analyzed using Perceptual Mapping. From the results of the study concluded that instant ginger of CV Intrafood product ranks second of four instant ginger products are compared. Attributes that can be used as a major force in improving product marketing instant ginger CV. Intrafood is the benefits of the product. The weakest side is the packaging design and performance attributes of the product in the packaging.

  6. New Missions for the CV-22 "OSPREY" in a Changing World

    National Research Council Canada - National Science Library

    Settergren, Daniel

    2000-01-01

    The CV-22 OSPREY will become operational within the next few years. Although it is seen as a replacement for the aging, but still capable, MH-53J PAVELOW, the CV-22 improves upon the basic operating capabilities of its predecessor...

  7. How Adequate is your CV? Analyzing French CVs with ReaderBench

    NARCIS (Netherlands)

    Gutu, Gabriel; Dascalu, Mihai; Trausan-Matu, Stefan; Lepoivre, Olivier

    2017-01-01

    This study is aimed at presenting a new ReaderBench-based tool built to support candidates in increasing the quality of their CV for a job opening. Both the visual quality and the textual content are considered while also providing an overview and corresponding feedback for the entire CV.

  8. Inoculación de rizobacterias promotoras del crecimiento vegetal en tomate (Solanum lycopersicum L. cv. afrodita en invernadero

    Directory of Open Access Journals (Sweden)

    Bernardo Espinosa Palomeque

    2017-04-01

    Full Text Available La producción de los cultivos agrícolas, entre otros factores, es impactada por el clima, el suelo, el agua y los microorganismos rizosféricos. De estos últimos, las rizobacterias promotoras del crecimiento vegetal (RPCV, desempeñan funciones importantes para las plantas como es la producción de reguladores del crecimiento vegetal y disminuir o prevenir los efectos de microorganismos f itopatógenos. El objetivo de este estudio fue evaluar el efecto de la inoculación de tres RPCV: Bacillus spp., Aeromonas spp., y Pseudomonas lini Delorme, utilizando dos sustratos: 1 compost+arena de río+perlita; y 2 arena de río; y como testigos ambos sustratos sin RPCV (lo que dio un total de 8 tratamientos, sobre el rendimiento y calidad de frutos de tomate (Solanum lycopersicum L. cv. Afrodita desarrollado bajo condiciones de invernadero. El diseño experimental utilizado fue bloques completamente al azar con cinco repeticiones, con arreglo factorial 2 × 4, los factores fueron: A sustratos y B RPCV. Las variables evaluadas en fruto fueron: diámetro polar y ecuatorial, espesor de pericarpio, contenido de sólidos solubles, firmeza, fenoles totales y capacidad antioxidante, así como rendimiento total. Los datos fueron analizados estadísticamente por análisis de varianza y las comparaciones de medias mediante la prueba de DMS 0.05%. Los frutos del tratamiento T1 (50 compost + 40 arena de río + 10 perlita + Bacillus spp. presentaron los mayores diámetros polar y ecuatorial, contenido de sólidos solubles, firmeza, contenido de fenoles totales y capacidad antioxidante, 6.54 cm, 5.50 cm, 5.94 ºBrix, 12.54 N, 51.70 mg de AG 100 g-1 FF y 66.68 µM Trolox g-1 FF, respectivamente; en comparación con el resto de los tratamientos. La aplicación de RPCV y la utilización de sustratos a base de compost podrían ser una alternativa de manejo en la producción de tomate en invernadero para incrementar el rendimiento y la calidad nutracéutica de los

  9. Rumen parameters of sheep fed Arachis pintoi cv. Belmonte hay

    Directory of Open Access Journals (Sweden)

    Gisele Machado Fernandes

    2012-12-01

    Full Text Available Studies on animal nutrition have shown factors that affect livestock yield and their interaction with the environment, through assessments on food nutritional values, nutrient metabolism in livestock and biochemical parameters related to nutrition and animal breeding. Inclusion levels of hay Arachis pintoi cv. Belmonte in the diet of sheep were studied by measuring the dry matter intake (DMI, production of short chain fatty acids (SCFA, ammonia nitrogen (NH3-N and rumen pH. Four males Santa Inês sheep with cannulas in the rumen were used in a 4x4 Latin Square design with four periods (21 days each and four treatments that corresponded to the inclusion levels (0%, 30%, 60% and 100% of Arachis pintoi cv. Belmonte to replace grass hay Cynodon dactylon cv. Coastcross in the diet. The DMI showed a quadratic effect increased until treatment with 60% of Arachis and reduced in the treatment with 100% of the legume. Significant interaction was observed between treatments and sampling times for NH3-N and acetate, propionate and butyrate concentration and the acetate:propionate ratio. There was no interaction between treatments and sampling time for rumen pH and total VFA concentration. It is observed a rapid increase in ammonia concentration until 2 hours after the feeding, and then a decline is seen. At 8 hours after the feeding, the quadratic effect occurred (P0.05 increasing NH3-N concentration with 60% of Arachis, declining afterwards. The acetate, propionate and butyrate concentration, showed a quadratic effect (P0.01 in the sampling time after the feeding for treatment with 60% of Arachis with the concentration rising until 4 hours and declining thereafter. For total SCFA concentration, when analyzed individually, we observed the effect of sampling time (P0.05, in which the means showed a quadratic effect, with maximum increase until 4 hours after the feeding, reducing at 8 hours. For acetate:propionate ratio was a linear effect (P0

  10. Rat and poultry feeding studies with soybean meal produced from imidazolinone-tolerant (CV127) soybeans.

    Science.gov (United States)

    He, Xiaoyun; de Brum, Paulo A R; Chukwudebe, Amechi; Privalle, Laura; Reed, Andrew; Wang, Yanqing; Zhou, Cui; Wang, Cuiyan; Lu, Jing; Huang, Kunlun; Contri, Daniela; Nakatani, Andreia; de Avila, Valdir S; Klein, Claudete H; de Lima, Gustavo J M M; Lipscomb, Elizabeth A

    2016-02-01

    The safety and nutritional properties of CV127 soybeans were evaluated in rat and broiler feeding studies. Some episodic differences were observed between rats fed CV127, Conquista, and the standard diet for the endpoints examined. None of these differences were considered treatment related, adverse, or biologically meaningful. In general, birds fed diets containing CV127, Conquista, or Monsoy 8001 showed no significant differences in growth and performance response variables. Chickens fed diets containing Coodetec 217 had lower body weight and weight gain for all developmental periods compared to CV127, but no significant differences were found in feed conversion for the two diets during any development period. The results of both feeding studies demonstrate that CV127 soybeans are as safe, wholesome, and nutritionally valuable as the other soybean meals tested, including those varieties for which histories of safe use have been established and well documented. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Detection of Cryptosporidium spp., Entamoeba spp. and Monocercomonas spp. in the gastrointestinal tract of snakes by in-situ hybridization.

    Science.gov (United States)

    Richter, B; Kübber-Heiss, A; Weissenböck, H; Schmidt, P

    2008-01-01

    This report describes the development of a diagnostic method for protozoal infections of the gastrointestinal tract of captive snakes, based on chromogenic in-situ hybridization with probes designed for the detection of 18S rRNA genes from Cryptosporidium spp., Entamoeba spp., Entamoeba invadens and Monocercomonas spp. The specificity of the probes was confirmed with the help of parasitic cultures and gene sequence analysis. The probes gave clear positive signals. Of 182 snakes examined, seven were positive with the Cryptosporidium probe, 13 with the Entamoeba probe (of which nine were also positive with the E. invadens probe), and 34 with the Monocercomonas probe.

  12. ANALISIS KELAYAKAN INVESTASI PENAMBAHAN MESIN FRAIS BARU PADA CV. XYZ

    Directory of Open Access Journals (Sweden)

    Febri Muhammad Rachadian

    2013-04-01

    Full Text Available Perkembangan teknologi sangat pesat sehingga dunia perindustrian harus mengikuti perkembangannya. Penerapan teknologi diharapkan dapat meningkatkan proses produksi sehingga dapat menghasilkan produk yang berkualitas. Pada industri pembuatan mesin cetak di CV XYZ, mesin-mesin yang digunakan telah hampir lebih dari 20 tahun. Ada indikasi bahwa mesin-mesin tersebut sudah lewat umur pakainya. Alternatif yang digunakan selama ini ialah penggantian komponen yang rusak. Peneliti ingin membandingkan alternative tersebut dengan  penambahan mesin frais baru. Untuk itu diperlukan analisis kelayakan investasi penambahan mesin baru. Dalam perkiraan kurun waktu 10 tahun dan suku bunganya 15 % dengan menggunakan NPV, penambahan mesin frais menarik sebesar Rp 461.201.000 dibandingkan penggantian komponen yang rusak sebesar Rp 211.227.000. Sebaliknya pada PBP, penggantian komponen yang rusak masih mengungguli dengan 1 tahun 4 bulan dibandingkan penambahan mesin frais baru selama 2 tahun 1 bulan. Begitupun juga PI penggantian komponen yang rusak masih menarik sebesar 4,2 dibandingkan PI penambahan mesin frais baru sebesar 2,56. Dapat disimpulkan bahwa dalam jangka waktu 10 tahun, alternatif penggantian komponen yang rusak masih lebih baik dibandingkan penambahan mesin frais baru. Namun, untuk investasi jangka panjang, mungkin saja alternatif penambahan mesin frais baru lebih menarik. Kata Kunci: penggantian komponen rusak, mesin frais baru, mesin cetak Abstract The rapid development of technology makes the world of industry must follow its development. Application of technology is expected to improve the production process so it can be produce a quality product. On the industrial manufacture of printing machines in CV XYZ, the machines have been used almost more than 20 years. There are indications that those machines passed the age lifetime. The alternatives that have been used during this time are the replacement of defective parts. Researchers wanted to

  13. MICROPROPAGAÇÃO DO KIWI CV. HAYWARD

    Directory of Open Access Journals (Sweden)

    SCALIZE FÁBIO EDUARDO

    2001-01-01

    Full Text Available O presente trabalho teve como objetivo desenvolver um protocolo para a obtenção de mudas de kiwi (Actinidia chinensis Planch, cv. Hayward, por meio do cultivo in vitro de cotilédones. Utilizou-se o meio de MURASHIGE & SKOOG (1962 -- MS, suplementado com dois tipos de auxina (AIA e AIB e uma citocinina (BAP. Foram verificados os efeitos de três doses de auxinas (0,125; 0,250 e 0,375 mg.L-1, combinadas com três doses de citocinina (0,5; 1,0 e 1,5 mg.L-1 na capacidade morfogênica dos explantes. Procedeu-se o estudo histológico dos órgãos das plântulas obtidas in vitro, e verificou-se, também, a capacidade de aclimatação das mudas ex vitro. A menor dose de AIB (0,125 mg.L-1, independentemente das doses de BAP, foi a mais eficaz na morfogênese dos explantes. Não foram verificadas alterações histológicas e anatômicas das plântulas obtidas in vitro. Aos três meses após o cultivo ex vitro dos explantes, verificou-se a sobrevivência de 88% das plantas transplantadas em condições de campo.

  14. EVALUASI SISTEM INFORMASI PERSEDIAAN PADA CV. SARANA TELEMAXINDO

    Directory of Open Access Journals (Sweden)

    Nelly Nelly

    2010-10-01

    Full Text Available Evaluation objectives of inventory information systems at CV. Facilities Telemaxindo was to evaluate the weaknesses of the implementation of internal control, as well as estimating and assessing possible risks that might arise. The research method used is literature study of reference books in the library, and field study consisted of observation, interviews with relevant parties, creating check lists, check the inventory information systems documentation, and testing of inventory application systems. The results are findings of control weaknesses in the implementation of security management, operational management control, boundary control, input control, and output control on the present inventory information system. These weaknesses could pose a risk and if not dealt soon it could hurt the company. The resulting conclusion of the security management control, boundary control, control input, and output control is not running well so that it needs to be improved in accordance with the recommendations, while operational management control has been running fairly well.Keywords: evaluation, information system, inventory

  15. A virtual computing infrastructure for TS-CV SCADA systems

    CERN Document Server

    Poulsen, S

    2008-01-01

    In modern data centres, it is an emerging trend to operate and manage computers as software components or logical resources and not as physical machines. This technique is known as â€ワvirtualisation” and the new computers are referred to as â€ワvirtual machines” (VMs). Multiple VMs can be consolidated on a single hardware platform and managed in ways that are not possible with physical machines. However, this is not yet widely practiced for control system deployment. In TS-CV, a collection of VMs or a â€ワvirtual infrastructure” is installed since 2005 for SCADA systems, PLC program development, and alarm transmission. This makes it possible to consolidate distributed, heterogeneous operating systems and applications on a limited number of standardised high-performance servers in the Central Control Room (CCR). More generally, virtualisation assists in offering continuous computing services for controls and maintaining performance and assuring quality. Implementing our systems in a vi...

  16. Antidiabetic and anticancer activities of Mangifera indica cv. Okrong leaves

    Science.gov (United States)

    Ganogpichayagrai, Aunyachulee; Palanuvej, Chanida; Ruangrungsi, Nijsiri

    2017-01-01

    Diabetes and cancer are a major global public health problem. Plant-derived agents with undesirable side-effects were required. This study aimed to evaluate antidiabetic and anticancer activities of the ethanolic leaf extract of Mangifera indica cv. Okrong and its active phytochemical compound, mangiferin. Antidiabetic activities against yeast α-glucosidase and rat intestinal α-glucosidase were determined using 1 mM of p-nitro phenyl-α-D-glucopyranoside as substrate. Inhibitory activity against porcine pancreatic α-amylase was performed using 1 mM of 2-chloro-4 nitrophenol-α-D-maltotroside-3 as substrate. Nitrophenol product was spectrophotometrically measured at 405 nm. Anticancer activity was evaluated against five human cancer cell lines compared to two human normal cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Mango leaf extract and mangiferin exhibited dose-dependent inhibition against yeast α-glucosidase with the IC50 of 0.0503 and 0.5813 mg/ml, respectively, against rat α-glucosidase with the IC50 of 1.4528 and 0.4333 mg/ml, respectively, compared to acarbose with the IC50 of 11.9285 and 0.4493 mg/ml, respectively. For anticancer activity, mango leaf extract, at ≥200 μg/ml showed cytotoxic potential against all tested cancer cell lines. In conclusion, mango leaf possessed antidiabetic and anticancer potential in vitro. PMID:28217550

  17. Future Objectives of Maintenance Management in the CV Group

    CERN Document Server

    Annila, L

    1999-01-01

    The ST/CV group has an extensive maintenance contract with a multinational consortium (Gematec-F/I/DE). Annually, the contract costs the group about 6 MCHF. Today the technical monitoring of the performance within the contract has become too irregular and different people's roles have not been sufficiently well defined. It has therefore been decided that all the technical, financial and performance-indicating data should be centrally co-ordinated within the group. The objective is to enable an efficient follow-up of the contract with a view to optimization. Another objective is to migrate to the new, more proficient version of the Computer Aided Maintenance Management (CAMM) software and learn to use it to its full potential. This means using its statistics and analysis features in addition to controlling the maintenance activities, spare-parts store, costs, etc. A further aim is to develop standards for the maintenance and the CAMM. This paper will discuss these objectives and the schedule for their implemen...

  18. Nitrogen fertilization on the establishment of Arachis pintoi cv. Belmonte

    Directory of Open Access Journals (Sweden)

    Rita Manuele Porto Sales

    2012-11-01

    Full Text Available The objective was to evaluate the effect of nitrogen fertilization on the establishment of forage peanut (Arachis pintoi cv. Belmonte propagated vegetatively. The experiment was conducted in a greenhouse in a completely randomized design with treatments arranged in a 2 × 4 factorial design - two ages (70 and 85 days after planting and four nitrogen doses (0, 40, 80 and 120 kg/ha - with four replications. Morphogenetic and structural characteristics and production were evaluated. The nitrogen accelerated the establishment of the forage peanut with an increase in dry weight of green leaves and stolons. The greatest length of stolons (48.0 cm was obtained with a dose equivalent to 86 kg N/ha and higher density of stolons (20 stolons/vase between 78 and 82 kg N/ha. Nitrogen fertilization also reduced the phyllochron from 6.7 to 4.6 days/leaf. These data were more intense at 85 days, suggesting greater photosynthetic contribution during this period related to the large number of leaves after 70 days. Therefore, nitrogen can be an important tool to accelerate the establishment of pure stands of forage peanut.

  19. Characterization of esterases from Cucurbita pepo cv. "Eskandrani".

    Science.gov (United States)

    Fahmy, Afaf S; Abo-Zeid, Amal Z; Mohamed, Tarek M; Ghanem, Hala M; Borai, Ibrahim H; Mohamed, Saleh A

    2008-01-01

    Two of the six esterases identified in Cucurbita pepo cv. "Eskandrani" were purified to homogeneity using two chromatography steps: anion exchange and gel filtration. The molecular weights of C. pepo esterases EIc and EII were 50,000 +/- 1500 and 68,000 +/- 1900 Da from gel filtration and 47,000 and 66,000 Da from SDS/PAGE, respectively, suggesting a monomeric structure for both enzymes. Esterases EIc and EII had K(m) values of 1.22 and 1.56 mM and pH optima at 9.0 and 8.0, respectively. The substrate specificity of C. pepo esterases EIc and EII were determined for a number of p-nitrophenyl esters, where their affinity toward these substrates were decreased as carbon atom number increased. Esterases EIc and EII had the same temperature optima, 40 degrees C. Thermal stability studies of esterases EIc and EII indicated that half maximal activities of EIc and EII esterases were reached at 55 degrees C and 50 degrees C, while they lost 45%, 51% and 70%, 77% of their activities after 30 and 90 min of incubation at 40 degrees C, respectively. The effect of different metal cations and inhibitors were examined. The inhibition studies revealed that the active sites of the two esterases contain serine and cysteine residues. The characteristics of C. pepo esterases are closely similar to those of microbial esterases used in food processing and food industry.

  20. Consolidation des installations ST/CV du complexe PS

    CERN Document Server

    Kühnl-Kinel, J

    2001-01-01

    Le PS (Synchrotron à Protons) a été inauguré officiellement le 5 février 1960 et a donné naissance au complexe des machines, lequel a évolué au fil des années. L'augmentation du nombre d'accélérateurs faisant partie du complexe PS, et leurs puissances électriques absorbées, suscite une croissance élevée en besoin de refroidissement. Cette dernière passe de 2 MW en 1960 à 25 MW à la fin des années 70, pour arriver au chiffre de 55 MW en 1998. Durant toutes ces années, les services techniques, hormis la construction des nouvelles installations, maintiennent et exploitent leurs équipements existants malgré les nombreuses contraintes parmi lesquelles sont citées l'âge des installations et leur accessibilité. Pour faire face aux demandes permanentes, afin d'avoir une fiabilité maximum des installations ST/CV, il est nécessaire de les consolider continuellement. Dans le présent document, les travaux de consolidation de ces équipements en vue du futur programme de physique sont présent�...

  1. Campylobacter spp. and birds of prey.

    Science.gov (United States)

    Dipineto, Ludovico; De Luca Bossa, Luigi Maria; Russo, Tamara Pasqualina; Cutino, Eridania Annalisa; Gargiulo, Antonio; Ciccarelli, Francesca; Raia, Pasquale; Menna, Lucia Francesca; Fioretti, Alessandro

    2014-06-01

    A total of 170 birds of prey admitted to two Wildlife Rescue and Rehabilitation Centers of Italy were examined. Birds were divided by diurnal (n = 15) and nocturnal (n = 7) species, sampled by cloacal swabs, and examined for Campylobacter spp. by cultural and molecular methods. Campylobacter spp. were isolated in 43 out of the 170 (25.3%) birds of prey examined. Among these, 43/43 (100%) were identified as Campylobacter jejuni and 10/43 (23.3%) were identified as Campylobacter coli recovered from mixed infections. Diurnal birds of prey showed a significantly higher prevalence value (P = 0.0006) for Campylobacter spp. than did nocturnal birds of prey.

  2.   The fungus Trichoderma spp. on vegetables

    DEFF Research Database (Denmark)

    Winding, Anne

      The fungus Trichoderma spp. on vegetables   Anne Winding, National Environmental Research Institute, Roskilde, AU   Trichoderma spp. is a naturally occurring fungus in soil and T. harzianum is an active ingredient in microbial pest control agents (MPCA) active against root pathogenic fungi....... The MPCA is administered to the plants by watering. The fungus establishes in the root zone and exerts its beneficial effect by general increase of resistance against pathogenic fungi. The natural occurrence of Trichoderma spp. and the fate and survival of the introduced T. harzianum on vegetables...

  3. Anatomy and morphology character of five Indonesian banana cultivars (Musa spp. of different ploidy level

    Directory of Open Access Journals (Sweden)

    ISSIREP SUMARDI

    2010-04-01

    Full Text Available Sumardi I, Wulandari M (2011 Anatomy and morphology character of five Indonesian banana cultivars (Musa spp. of different ploidy level. Biodiversitas 12: 167-175. In Indonesia there are many cultivars of banana, and some of them produce edible fruits. Beside their morphology, the character which necessary as a tool for classification is anatomical character. The aim of this research were to describe the anatomical character and morphology of fives Indonesian banana cultivars based on their level of ploidy. The cultivars were collected from Banana Germplasm Plantation, Yogyakarta District, Indonesia. The samples of roots, rhizome, and leaf were collected from five banana cultivars i.e.: Musa acuminata cv Penjalin, M.balbisiana cv Kluthuk warangan, M.acuminata cv Ambon warangan, M.paradisiaca cv Raja nangka , and M. paradisiaca cv Kluthuk susu. For anatomy observation samples were prepared using paraffin method, stained with 1% safranin in 70% ethanol. To observe the structure of stomata and epidermis surface, slide were prepared using modification of whole mount method. Slides were observed using Olympus BHB microscope completed with Olympus camera BM-10A. Stem and leaf morphology character of diploid level (AA and BB genome is different with triploid level (AAA, AAB, and ABB genome. Anatomy and morphology character of root and rhizome of banana in diploid level (AA and BB genome and triploid level (AAA, AAB, and ABB genome is quite similar. Distribution of stomata is found in leaf and pseudostem. Stomata is found in adaxial and abaxial epidermis layer. The size of guard cells in triploid cultivars was longer than that diploid cultivars. The root composse of epidermis layer, cortex and cylinder vascular of five cultivar’s root show anomalous structure. Rhizome consist of peripheric and centre zone. Anatomically, this was no differences in the rizome structur among five banana cultivars. The row of vascular bundles act as demarcation area

  4. Assembly of the Lactuca sativa, L. cv. Tizian draft genome sequence reveals differences within major resistance complex 1 as compared to the cv. Salinas reference genome.

    Science.gov (United States)

    Verwaaijen, Bart; Wibberg, Daniel; Nelkner, Johanna; Gordin, Miriam; Rupp, Oliver; Winkler, Anika; Bremges, Andreas; Blom, Jochen; Grosch, Rita; Pühler, Alfred; Schlüter, Andreas

    2018-02-10

    Lettuce (Lactuca sativa, L.) is an important annual plant of the family Asteraceae (Compositae). The commercial lettuce cultivar Tizian has been used in various scientific studies investigating the interaction of the plant with phytopathogens or biological control agents. Here, we present the de novo draft genome sequencing and gene prediction for this specific cultivar derived from transcriptome sequence data. The assembled scaffolds amount to a size of 2.22 Gb. Based on RNAseq data, 31,112 transcript isoforms were identified. Functional predictions for these transcripts were determined within the GenDBE annotation platform. Comparison with the cv. Salinas reference genome revealed a high degree of sequence similarity on genome and transcriptome levels, with an average amino acid identity of 99%. Furthermore, it was observed that two large regions are either missing or are highly divergent within the cv. Tizian genome compared to cv. Salinas. One of these regions covers the major resistance complex 1 region of cv. Salinas. The cv. Tizian draft genome sequence provides a valuable resource for future functional and transcriptome analyses focused on this lettuce cultivar. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. [Study on antitumor effect and its toxicity of Ipomoea Batatas Poir Cv anthocyanins].

    Science.gov (United States)

    Liu, Ning; Wang, Hongbing; Wang, Chunbo

    2008-07-01

    To investigate the antitumor effect and estimate the toxicity of Ipomoea Batatas Poir Cv anthocyanins. Mice sarcinoma S180 and mice liver cancer H22 were administered with positive Ftorafur and different dose of Ipomoea Batatas Poir Cv anthocyanins by pouring into the stomach. The antitumor effects of Ipomoea Batatas Poir Cv anthocyanins were observed by calculating the inhibition rate. Subchronic toxicity tests and micronucleus test of bone marrow cell in mice and Ames test were carried out to evaluate the toxicity. At the doses of 150 mg and 75 mg Ipomoea Batatas Poir Cv anthocyanins, the rates of sarcinoma 180 inhibition were 45.04% and 36.64% respectively. The rate of liver cancer H22 inhibition at the dose of 150mg Batatas Poir Cv anthocyanins was 33.33% . The result of subchronic toxicity tests showed that Ipomoea Batatas Poir Cv anthocyanins had no obvious toxicity to rats. The results of the Ames test and micronucleus test were negative. Ipomoea Batatas Poir Cv anthocyanins could have inhibitory effect on transplantation tumor of mice, and have no toxicity and no mutation.

  6. Reclassification of Hart and Northwest Africa 6047: Criteria for distinguishing between CV and CK3 chondrites

    Science.gov (United States)

    Dunn, Tasha L.; Gross, Juliane

    2017-11-01

    The single parent body model for the CV and CK chondrites (Greenwood et al.) was challenged by Dunn et al., who argued that magnetite compositions could not be reconciled by a single metamorphic sequence (i.e., CV3 → CK3 → CK4-6). Cr isotopic compositions, which are distinguishable between the CV and CK chondrites, also support two different parent bodies (Yin et al.). Despite this, there are many petrographic and mineralogical similarities between the unequilibrated (petrologic type 3) CK chondrites and the CV chondrites (also type 3), which may result in misclassification of samples. Hart and Northwest Africa 6047 (NWA 6047) are an excellent example of this. In this study, we revisit the classification of Hart and NWA 6047 using magnetite compositions, petrography, and compositions of olivine, the most ubiquitous mineral in both CV and CK chondrites. Not only do our results suggest that NWA 6047 and Hart were misclassified, but our assessment of CV and CK3 chondrites has also led to the development of criteria that can be used to distinguish between CV and CK3 chondrites. These criteria include: abundances of Cr2O3, TiO2, NiO, and Al2O3 in magnetite; Fa content and NiO abundance of matrix olivine; FeO content of chondrules; and the chondrule:matrix ratio. Classification as a CV chondrite is also supported by the presence of igneous chondrule rims, calcium-aluminum-rich inclusions, and an elongated petrofabric. However, none of these petrographic characteristics can be used conclusively to distinguish between CV and CK3 chondrites.

  7. Karakteristik Tepung Prebiotik Umbi Uwi (Dioscorea Spp)

    OpenAIRE

    Winarti, Sri; Saputro, Erwan Adi

    2013-01-01

    Umbi uwi (Dioscorea spp.) merupakan salah satu jenis umbi yang banyak tumbuh di Indonesia memiliki kandungan karbohidrat yang tinggi. Keanekaragaman uwi sangat banyak baik dilihat dari bentuk, ukuran, warna, maupun rasa umbinya. Terdapat lebih dari 600 spesies dari genus Dioscorea spp. tersebar di berbagai negara, termasuk Indonesia, antara lain Dioscorea hispida (gadung), Dioscorea esculenta (gembili), Discorea bulbifera (gembolo), Dioscorea alata (uwi ungu/purple yam), Dioscorea opposita (...

  8. Genetic manipulation of Methanosarcina spp.

    Directory of Open Access Journals (Sweden)

    Petra Regine Adelheid Kohler

    2012-07-01

    Full Text Available The discovery of the third domain of life, the Archaea, is one of the most exciting findings of the last century. These remarkable prokaryotes are well known for their adaptations to extreme environments; however, Archaea have also conquered moderate environments. Many of the archaeal biochemical processes, such as methane production, are unique in nature and therefore of great scientific interest. Although formerly restricted to biochemical and physiological studies, sophisticated systems for genetic manipulation have been developed during the last two decades for methanogenic archaea, halophilic archaea and thermophilic, sulfur-metabolizing archaea. The availability of these tools has allowed for more complete studies of archaeal physiology and metabolism and most importantly provides the basis for the investigation of gene expression, regulation and function. In this review we provide an overview of methods for genetic manipulation of Methanosarcina spp., a group of methanogenic archaea that are key players in the global carbon cycle and which can be found in a variety of anaerobic environments.

  9. Differentiation of Phytopathogenic Agrobacterium spp.

    Directory of Open Access Journals (Sweden)

    Nemanja Kuzmanović

    2011-01-01

    Full Text Available Due to the difficulties in differentiation of phytopathogenic Agrobacterium spp. and lack of a standardized protocol, we carried out selection and evaluation of suitable methods based on the bacterial physiological, genetic and pathogenic properties. Strains of Agrobacterium tumefaciens, A. rhizogenes and A. vitis were differentiated using standard bacteriological and molecular methods. The biochemical and physiological tests confirmed authenticity of the strains. Two duplex PCR methods were conducted with four different primer pairs. In all strains, presence of plasmid virD2 and virC pathogenicity genes was detected. Chromosomal pehA gene was determined in A. vitis strain. Pathogenicity was confirmed on carrot slices and young plants of tomato and sunflower. Strains of A. tumefaciens and A. vitis were pathogenic on all test plants, while strain of A. rhizogenes induced characteristic symptoms only on carrot slices. The tests used in this study provided reliable discrimination between the three species and confirmed their identity as tumorigenic (TiAgrobacterium tumefaciens and A. vitis, and rhizogenic (Ri A. rhizogenes.

  10. ANALISIS PENGUKURAN PRODUKTIVITAS PADA CV. CITRA JEPARA FURNITURE

    Directory of Open Access Journals (Sweden)

    Darminto Pujotomo

    2012-02-01

    Full Text Available CV. Citra Jepara adalah perusahaan furnitur ekspor. Pada masa itu, perusahaan hanya melihat level keuntungan untuk mengkaji perusahaan telah berjalan lebih baik atau kebalikannya. Hasil persepsi membuat perusahaan hanya sedikit memperhatikan efisiensi pemakaian sumber daya yang dimiliki (input sehingga perusahaan membutuhkan biaya besar untuk melakukan aktivitas perusahaan.Penelitian ini mempelajari evaluasi produktivitas perusahaan dan pengukuran serta hubungan antara level produktivitas dengan level profitabilitas (kemampuan perusahaan untuk menciptakan laba yang dapat dicapai oleh perusahaan.Hasil penelitian ini tentang pengukuran produktivitas parsial memperlihatkan level produktivitas berubah-ubah dan masih memerlukan usaha peningkatan produktivitas parsial terutama dalam pemakaian masing-masing input dengan efisien. Level Total Factor Productivity selama perioda pengukuran memperlihatkan level pertumbuhan positif. Ini melukiskan perusahaan sukses dalam mengelola input tenaga kerja dan modal kolektif yang dimiliki. Level Total Productivity selama perioda pengukuran menunjukkan level pertumbuhan yang berubah-ubah. Ini melukiskan perusahaan itu belum dapat mengelola input yang dimiliki keseluruhan dengan sukses. Untuk melukiskan hubungan antara produktivitas total dengan profitabilitas dari hasil penelitian bisa diketahui faktor produktivitas (0,998 mempunyai pengaruh lebih besar dibandingkan pemulihan harga (0,457 untuk peningkatan profitabilitas perusahaan selama kurun waktu satu tahun perioda pengukuran. Diperlukan usaha untuk meningkatkan profitabilitas perusahaan untuk periode mendatang disertai usaha untuk meningkatkan produktivitas yang memusatkan pada peningkatan produktivitas material dan produktivitas modal sebagai input yang sangat mempengaruhi profitabilitas perusahaan dengan cara berkelanjutkan dan berkesinambungan. Kata kunci: pengukuran evaluasi dan produktivitas, profitabilitas    CV. Citra Jepara is an export furniture

  11. Novel circulatory connection from the acupoint Zhong Wan(CV12 to pancreas

    Directory of Open Access Journals (Sweden)

    Minsoo Kim

    2008-03-01

    Full Text Available Objectives : Demonstrating a novel circulatory path from the acupoint(CV12 to the pancreas. Method : Alcian blue(1% solution, 20μl, pH 7.4 was injected into the acupoint(CV12. Two hours later the surfaces of internal organs were observed by using a stereomicroscope. Results : Alcian blue arrived and colored the omental fat band(OFB on the pancreas. The OFB connected the head and tail of the pancreas, the pancreas and the spleen, and the pancreas and the stomach. Conclusion : The existence of a novel circulatory path from the acupoint CV12 to the pancreas and its OFB was demonstrated.

  12. Potential growing model for the standard carnation cv. Delphi

    Directory of Open Access Journals (Sweden)

    Miguel Ángel López M.

    2014-08-01

    Full Text Available The cut flower business requires exact synchronicity between product offer and demand in consumer countries. Having tools that help to improve this synchronicity through predictions or crop growth monitoring could provide an important advantage to program standards and corrective agronomic practices. At the Centro de Biotecnología Agropecuaria, SENA (SENA's Biotechnology, Agricultural and Livestock Center, located in Mosquera, Cundinamarca, a trial with standard carnation cv. Delphi grown under greenhouse conditions was carried out. The objective of this study was to build a simple model of dry matter (DM production and partition of on-carnation flower stems. The model was based on the photosynthetically active radiation (PAR MJ m-2 d-1 and temperature as exogenous variables and assumed no water or nutrient limitations or damage caused by pests, disease or weeds. In this model, the daily DM increase depended on the PAR, the light fraction intercepted by the foliage (F LINT and the light use efficiency (LUE g MJ-1. The LUE in the vegetative and reproductive stages reached values of 1.31 and 0.74 g MJ-1, respectively. The estimated extinction coefficient (k value corresponded to 0.53 and the maximum F LINT was between 0.79 and 0.82. Partitioning between the plant vegetative and reproductive stages was modeled based on the hypothesis that the partition is regulated by the source sink relationship. The estimated partition coefficient for the vegetative stage of the leaves was 0.63 and 0.37 for the stems. During the reproductive stage, the partitioning coefficients of leaves, stems and flower buds were 0.05, 0.74, and 0.21, respectively.

  13. Effect of Sodium Cyanide on Wheat (Triticum durum cv. Altar and T. aestivum cv. Cumhuriyet)

    DEFF Research Database (Denmark)

    Gemici, Meliha; Karshenass, A.M.; Tan, Kit

    2008-01-01

    The effect of sodium cyanide on the morphology of stem, leaves and grain yields of Triticum durum cv. Altar and Triticum aestivum cv. Cumhuriyet grown under glass was studied. Seeds were planted in six different sets of pots containing ordinary garden soil. After formation of the first leaves...... of individual plants was monitored until grain production. It was found that the sodium cyanide concentrations in the feed solutions affected plant stature, with the plants becoming progressively dwarfed with increasing dosage. Anomalies in the morphological and anatomical structure of the plant were also noted...

  14. Occurrence of Babesia spp., Rickettsia spp. and Bartonella spp. in Ixodes ricinus in Bavarian public parks, Germany

    Directory of Open Access Journals (Sweden)

    Mahling Monia

    2011-07-01

    Full Text Available Abstract Background Only limited information is available about the occurrence of ticks and tick-borne pathogens in public parks, which are areas strongly influenced by human beings. For this reason, Ixodes ricinus were collected in public parks of different Bavarian cities in a 2-year survey (2009 and 2010 and screened for DNA of Babesia spp., Rickettsia spp. and Bartonella spp. by PCR. Species identification was performed by sequence analysis and alignment with existing sequences in GenBank. Additionally, coinfections with Anaplasma phagocytophilum were investigated. Results The following prevalences were detected: Babesia spp.: 0.4% (n = 17, including one pool of two larvae in 2009 and 0.5 to 0.7% (n = 11, including one pool of five larvae in 2010; Rickettsia spp.: 6.4 to 7.7% (n = 285, including 16 pools of 76 larvae in 2009. DNA of Bartonella spp. in I. ricinus in Bavarian public parks could not be identified. Sequence analysis revealed the following species: Babesia sp. EU1 (n = 25, B. divergens (n = 1, B. divergens/capreoli (n = 1, B. gibsoni-like (n = 1, R. helvetica (n = 272, R. monacensis IrR/Munich (n = 12 and unspecified R. monacensis (n = 1. The majority of coinfections were R. helvetica with A. phagocytophilum (n = 27, but coinfections between Babesia spp. and A. phagocytophilum, or Babesia spp. and R. helvetica were also detected. Conclusions I. ricinus ticks in urban areas of Germany harbor several tick-borne pathogens and coinfections were also observed. Public parks are of particularly great interest regarding the epidemiology of tick-borne pathogens, because of differences in both the prevalence of pathogens in ticks as well as a varying species arrangement when compared to woodland areas. The record of DNA of a Babesia gibsoni-like pathogen detected in I. ricinus suggests that I. ricinus may harbor and transmit more Babesia spp. than previously known. Because of their high recreational value for human beings, urban green

  15. Occurrence of Babesia spp., Rickettsia spp. and Bartonella spp. in Ixodes ricinus in Bavarian public parks, Germany.

    Science.gov (United States)

    Schorn, Sabine; Pfister, Kurt; Reulen, Holger; Mahling, Monia; Silaghi, Cornelia

    2011-07-15

    Only limited information is available about the occurrence of ticks and tick-borne pathogens in public parks, which are areas strongly influenced by human beings. For this reason, Ixodes ricinus were collected in public parks of different Bavarian cities in a 2-year survey (2009 and 2010) and screened for DNA of Babesia spp., Rickettsia spp. and Bartonella spp. by PCR. Species identification was performed by sequence analysis and alignment with existing sequences in GenBank. Additionally, coinfections with Anaplasma phagocytophilum were investigated. The following prevalences were detected: Babesia spp.: 0.4% (n = 17, including one pool of two larvae) in 2009 and 0.5 to 0.7% (n = 11, including one pool of five larvae) in 2010; Rickettsia spp.: 6.4 to 7.7% (n = 285, including 16 pools of 76 larvae) in 2009. DNA of Bartonella spp. in I. ricinus in Bavarian public parks could not be identified. Sequence analysis revealed the following species: Babesia sp. EU1 (n = 25), B. divergens (n = 1), B. divergens/capreoli (n = 1), B. gibsoni-like (n = 1), R. helvetica (n = 272), R. monacensis IrR/Munich (n = 12) and unspecified R. monacensis (n = 1). The majority of coinfections were R. helvetica with A. phagocytophilum (n = 27), but coinfections between Babesia spp. and A. phagocytophilum, or Babesia spp. and R. helvetica were also detected. I. ricinus ticks in urban areas of Germany harbor several tick-borne pathogens and coinfections were also observed. Public parks are of particularly great interest regarding the epidemiology of tick-borne pathogens, because of differences in both the prevalence of pathogens in ticks as well as a varying species arrangement when compared to woodland areas. The record of DNA of a Babesia gibsoni-like pathogen detected in I. ricinus suggests that I. ricinus may harbor and transmit more Babesia spp. than previously known. Because of their high recreational value for human beings, urban green areas are likely to remain in the research focus on

  16. Epidemiology of Salmonella spp., Listeria monocytogenes and Campylobacter spp., in the poultry chain production system

    Directory of Open Access Journals (Sweden)

    Realpe-Delgado, María Elena

    2016-10-01

    Full Text Available Salmonella spp., Campylobacter spp., and L. monocytogenes are zoonotic foodborne pathogens, associated with the consumption of contaminated foods of animal origin. In this study we determined the prevalence and risk factors associated with the presence of these microorganisms at all stages of the production system, in two Colombian poultry companies (EI-EI-I and II. In EI-I, Campylobacter spp., and Salmonella spp., were isolated from 10 % and 4.4 % of the specimens, and S. Heidelberg was the predominant serotype. Salmonella spp., was found in 6 % of hands and stool samples of workers. S. Saphra was the most prevalent serotype. In EI-II, the prevalence of Campylobacter spp., and Salmonella spp., from animal specimens was 7 % and 17 %, respectively. L. monocytogenes was not detected. This study established the prevalence of these zoonotic pathogens through the production chain and showed the presence of pathogen carriers among workers/food handlers. “Lack of medical examination of employees in the previous year” was found to be a possible risk factor for carriage of Salmonella spp.

  17. Isothermal microcalorimetry for antifungal susceptibility testing of Mucorales, Fusarium spp., and Scedosporium spp.

    NARCIS (Netherlands)

    Furustrand Tafin, U.; Meis, J.F.G.M.; Trampuz, A.

    2012-01-01

    We evaluated isothermal microcalorimetry for real-time susceptibility testing of non-Aspergillus molds. MIC and minimal effective concentration (MEC) values of Mucorales (n = 4), Fusarium spp. (n = 4), and Scedosporium spp. (n = 4) were determined by microbroth dilution according to the Clinical

  18. Implementation Yamaha Motorcycle Sales Promotion in the CV. Tri Star Kencana Bangkinang Kampar District

    OpenAIRE

    Ibrahim, Mariaty; Diana, Nova

    2015-01-01

    Sales promotion is a seriesof actions to get the attention of consumers and provide stimulus to prospective customers. Integrative designed to promote and offer items that are considered by customers differ in important and unique to them. This research was conducted at CV. Tri Star Kencana Bangkinang Kampar District, the objective of this study is to investigate the implementation of the campaign carried out by CV. Tri Star Kencana Bangkinang and to know the problems found by using quantitat...

  19. Evaluation of faba beans ( Vicia faba cv. Fiord) and sweet lupins ...

    African Journals Online (AJOL)

    Evaluation of faba beans ( Vicia faba cv. Fiord) and sweet lupins ( Lupizus albus cv. Kiev) as protein sources for ... P igsi n the metabolisrna ndN balance trial consurned5 % less( P < 0.01)o 1'thel upind ietc omparedto the fabab eand iet (l 370 vs. | 440 gld).N o significandt ift'erenceisn the digestiblee nergy( DE) contenta nd ...

  20. Pengaruh Kualitas Pelayanan terhadap Loyalitas Pelanggan di Rental Mobil CV. Ilham Motor Gandu Mlarak Ponorogo

    OpenAIRE

    Dhika Amalia

    2015-01-01

    The purpose of this study was to determine the amount of quality customer service and loyalty to know a very dominant factor affecting customer loyalty in the CV . Ilham Motor. This research method is the primary data from the questionnaires , sampling technique is a technique associated with customer 100 CV Ilham Motor . Data analysis is a quantitative method to test the validity , reliability testing , multiple linear regression analysis , the correlation coefficient analytic , analytic det...

  1. Effect of Sales Promotion Costs Yamaha Motorcycle Brand in Scorpio CV. Jadi Cemerlang Jaya Pekanbaru

    OpenAIRE

    Rikson, Andi; ', Jushermi; Nursanti, Aida

    2014-01-01

    This research is conducted to determine the effect of Promotion Cost (CostAdvertising, Sales Promotion Costs, and Cost Personal Selling) Motorcycles for Sale Yamaha Scorpio Brands at CV. Jadi Cemerlang Jaya Pekanbaru and promotion costs to determine which is the most dominant effect on the Yamaha Motorcycle Sales BrandScorpio at CV. Jadi Cemerlang Jaya Pekanbaru. To analyze the data, in this study used linear regression test is to prove the direction of the influence of service as an independ...

  2. Identification and discrimination of Toxoplasma gondii, Sarcocystis spp., Neospora spp., and Cryptosporidium spp. by righ-resolution melting analysis.

    Directory of Open Access Journals (Sweden)

    Hllytchaikra Ferraz Fehlberg

    Full Text Available The objective of this study was to standardize the high-resolution melting method for identification and discrimination of Toxoplasma gondii, Sarcocystis spp., Neospora spp., and Cryptosporidium spp. by amplification of 18S ribosomal DNA (rDNA using a single primer pair. The analyses were performed on individual reactions (containing DNA from a single species of a protozoan, on duplex reactions (containing DNA from two species of protozoa in each reaction, and on a multiplex reaction (containing DNA of four parasites in a single reaction. The proposed method allowed us to identify and discriminate the four species by analyzing the derivative, normalized, and difference melting curves, with high reproducibility among and within the experiments, as demonstrated by low coefficients of variation (less than 2.2% and 2.0%, respectively. This is the first study where this method is used for discrimination of these four species of protozoa in a single reaction.

  3. Identification and discrimination of Toxoplasma gondii, Sarcocystis spp., Neospora spp., and Cryptosporidium spp. by righ-resolution melting analysis.

    Science.gov (United States)

    Fehlberg, Hllytchaikra Ferraz; Maciel, Bianca Mendes; Albuquerque, George Rêgo

    2017-01-01

    The objective of this study was to standardize the high-resolution melting method for identification and discrimination of Toxoplasma gondii, Sarcocystis spp., Neospora spp., and Cryptosporidium spp. by amplification of 18S ribosomal DNA (rDNA) using a single primer pair. The analyses were performed on individual reactions (containing DNA from a single species of a protozoan), on duplex reactions (containing DNA from two species of protozoa in each reaction), and on a multiplex reaction (containing DNA of four parasites in a single reaction). The proposed method allowed us to identify and discriminate the four species by analyzing the derivative, normalized, and difference melting curves, with high reproducibility among and within the experiments, as demonstrated by low coefficients of variation (less than 2.2% and 2.0%, respectively). This is the first study where this method is used for discrimination of these four species of protozoa in a single reaction.

  4. Activation of bovine neutrophils by Brucella spp.

    Science.gov (United States)

    Keleher, Lauren L; Skyberg, Jerod A

    2016-09-01

    Brucellosis is a globally important zoonotic infectious disease caused by gram negative bacteria of the genus Brucella. While many species of Brucella exist, Brucella melitensis, Brucella abortus, and Brucella suis are the most common pathogens of humans and livestock. The virulence of Brucella is largely influenced by its ability to evade host factors, including phagocytic killing mechanisms, which are critical for the host response to infection. The aim of this study was to characterize the bovine neutrophil response to virulent Brucella spp. Here, we found that virulent strains of smooth B. abortus, B. melitensis, B. suis, and virulent, rough, strains of Brucella canis possess similar abilities to resist killing by resting, or IFN-γ-activated, bovine neutrophils. Bovine neutrophils responded to infection with a time-dependent oxidative burst that varied little between Brucella spp. Inhibition of TAK1, or SYK kinase blunted the oxidative burst of neutrophils in response to Brucella infection. Interestingly, Brucella spp. did not induce robust death of bovine neutrophils. These results indicate that bovine neutrophils respond similarly to virulent Brucella spp. In addition, virulent Brucella spp., including naturally rough strains of B. canis, have a conserved ability to resist killing by bovine neutrophils. Copyright © 2016 Elsevier B.V. All rights reserved.

  5. Widespread Oceanospirillaceae Bacteria in Porites spp.

    Directory of Open Access Journals (Sweden)

    Mark D. Speck

    2012-01-01

    Full Text Available We present evidence that a clade of bacteria in the Oceanospirillaceae is widely distributed in Porites spp. and other hermatypic corals. Bacteria 16S rDNA clone libraries were prepared from community genomic DNA extracted from Porites compressa and Porites lobata surface mucus and adjacent seawater collected along a line transect off Maui. Phylogenetic affiliations of operational taxonomic units (OTUs defined at the 97% level of nucleotide identity varied within and between the respective Porites spp. along the transect and differed from those in the seawater. One OTU (C7-A01, however, occurred in all mucus samples from both Porites species. C7-A01c affiliates with a clade of uncultivated Oceanospirillum-like bacteria; the nearest neighbors of this OTU have been reported only in the surface mucus layer of Porites spp. and other stony corals, in reef-dwelling invertebrates, and the corallivorous six-banded angelfish, Pomacanthus sexstriatus.

  6. Prevalence of Brucella spp in humans

    Directory of Open Access Journals (Sweden)

    Catharina de Paula Oliveira Cavalcanti Soares

    2015-10-01

    Full Text Available Objective: to determine the seroprevalence of Brucella spp in humans.Method: this is an observational study, developed with 455 individuals between 18 and 64 years old, who use the Estratégia de Saúde da Família (Brazil's family health strategy. The serum samples of volunteers underwent buffered acid antigen tests, such as screening, agar gel immunodiffusion and slow seroagglutination test in tubes and 2-Mercaptoethanol.Results: among the samples, 1.98% has responded to buffered-acid antigen, 2.85% to agar gel immunodiffusion test and 1.54% to the slow seroagglutination tests on tubes/2-Mercaptoethanol. The prevalence of Brucella spp was 4.4%, represented by the last two tests.Conclusion: the results of this research suggest that the studied population is exposed to Brucella spp infection.

  7. Association of Treponema spp. with canine periodontitis.

    Science.gov (United States)

    Nordhoff, Marcel; Rühe, Bärbel; Kellermeier, Claudia; Moter, Annette; Schmitz, Rose; Brunnberg, Leo; Wieler, Lothar H

    2008-03-18

    To evaluate the association of oral Treponema (T.) spp. with severity of canine periodontitis, subgingival plaque samples of dogs of various breeds undergoing surgery were investigated. A wide range of oral Treponema spp. was analysed by a molecular and culture-independent approach applying DNA-DNA dot blot hybridization analysis and fluorescence in situ hybridization using Treponema specific oligonucleotide probes specific for phylogenetic groups I-VII of oral treponemes as well as probes specific for T. socranskii and T. denticola. To assess the periodontal status of affected dogs clinical parameters were measured and the periodontal status was classified from grade 0 (physiological periodont) to 3 (severe periodontitis). The periodontal status correlated significantly with an increasing concentration of volatile sulfur compounds (VSC, r=0.854) determined with a Halimeter, indicating a positive correlation between the presence of VSC-producing bacteria and periodontitis. In this study Treponema spp. of phylogenetic groups III, V-VII were not detected in any sample, whereas T. denticola-like treponemes were found only in 2 of 51 animals. However, treponemes belonging to phylogenetic groups I, II and IV of oral treponemes or T. socranskii were found in up to 64.84% of the dogs. The detection rate of Treponema spp. was significantly associated with an increased periodontal status. Treponemes present in periodontal lesions were also visualized by fluorescence in situ hybridization of gingival biopsies showing Treponema spp. not only in the microbial biofilm but also within the gingival tissue. The data presented here indicate that oral Treponema spp. are associated with canine periodontitis. Similar to human periodontitis, treponemes of groups I, II and IV and T. socranskii were found more frequently the higher the degree of periodontitis was.

  8. Prevalence of Salmonella spp. in reptiles

    OpenAIRE

    加藤, 行男; 村上, 賢

    2006-01-01

    A total of 291 fecal samples from 252 wild reptiles and 39 pet reptiles were examined for the prevalence of Salmonella spp. in Japan. Salmonella spp. were isolated from 29 (11.5%) of 252 wild reptiles and 22 (55.6%) of 39 pet reptiles. The isolates were identified into subspecies I to IV. The majority of isolates (43.6%) belonged to subspecies I and these isolates could be identified into 9 serovars. The serovars isolated were found to be S. Newport, S. Litchifield and S. Thompson which cause...

  9. Produtividade e valor nutritivo da Brachiaria brizantha cv. Marandu em um sistema silvipastoril Productivity and nutritional value of Brachiaria brizantha cv. Marandu in a silvopastoral system

    Directory of Open Access Journals (Sweden)

    L.F. Sousa

    2007-08-01

    Full Text Available O experimento foi realizado em sistema silvipastoril (Ssp localizado no bioma de Cerrado, no município de Lagoa Santa, Minas Gerais, no ano agrícola de 2004-2005. Avaliou-se a influência do Ssp composto pela arbórea bolsa-de-pastor (Zeyheria tuberculosa sobre os parâmetros produção, atributos morfogênicos, composição bromatológica e valor nutritivo da gramínea Brachiaria brizantha cv. Marandu, e os efeitos sobre as condições microclimáticas. O delineamento experimental foi em blocos ao acaso, com cinco blocos (épocas de corte, dois tratamentos (T1 - B. brizantha cv. Marandu sombreada com a Z. tuberculosa e T2 - B. brizantha cv. Marandu, a pleno sol e três repetições (parcelas. O sombreamento proporcionado pela Z. tuberculosa, apesar de reduzir a produção de matéria seca (MS da gramínea, favoreceu o aumento do teor de proteina bruta (PB e não alterou a produção de PB/ha e nem afetou a concentração de FDN. Os teores de FDA foram mais altos que os encontrados a pleno sol, o que provavelmente resultou em menor degradabilidade in vitro da MS e da matéria orgânica, não alterando a degradabilidade efetiva.The experiment was carried out in a Silvopastoral system (Sps located in Brazilian Savannah, Lagoa Santa county, Minas Gerais State, during the agricultural year of 2004-2005. It was studied the influence of Sps formed by "Bolsa de Pastor" (Zeyheria tuberculosa on the production, morphogenic attributes, bromatological composition and nutritional value of the graminaceous Brachiaria brizantha cv. Marandu as well as on the microclimatic aspects. The Sps was established in 1982, in a red-yellow latossol by the natural regeneration process. The experiment was based on a randomized block design, with 5 blocks (cutting times, 2 treatments (T1 - B. brizantha cv. Marandu shaded by Z. tuberculosa and T2 - B. brizantha cv. Marandu at open sky and three repetitions (plots. Although the DM production of the graminaceous in the Sps

  10. FORMULASI STRATEGI PENINGKATAN PRODUKSI DOMBA CV MITRA TANI FARM, CIAMPEA, BOGOR

    Directory of Open Access Journals (Sweden)

    Amalia Sholehana

    2014-04-01

    Full Text Available ABSTRACTThis research was conducted to formulate to increase the sheep production at CV Mitra Tani Farm (CV MT Farm, Ciampea, Bogor. The research was conducted using descriptive analysis by analyzing the problem experienced by CV. MitraTani Farm. Primary data and secondary data were gathered at this research. The research was also conducted using several analytical methods such as value chain mapping, internal factor evaluation (IFE and external factor evaluation (EFE matrix, SWOT and quantitative strategic planning matrix (QSPM. Based on the value chain analysis, the current production of sheep at CV MT farm is highly depending on the supply from its partners and internal farmers. According to the IFE and EFE results, the differences of each weighted values were respectively 2,120 and 0,686 so the position of the company in the SWOT matrix was situated at Quadrant I. Therefore the company needs to use a growth strategy. The QSPM sequenced the strategy priority as follow (1 increasing the number of lambs, (2 developing the management, (3 strengthening the capital and ownership, (4 improving the quality through technology. The result of the gross profit margin calculation, if the sheep‘s selling is increased up to 1000 sheep per month then the possible annual profit that can be earned by CV MT farm is 40,34% of the total production.Keywords: MT Farm, sheep production, strategies, SWOT, QSPM, gross profit marginABSTRAKPenelitian ini dilakukan untuk memformulasikan strategi peningkatan produksi domba di CV Mitra Tani Farm (CV MT Farm, Ciampea, Bogor. Pendekatan penelitian yang diterapkan adalah analisis deskriptif yang dilakukan dengan mempelajari permasalahan dari objek yang diteliti. Data yang dikumpulkan dalam penelitian ini adalah data primer dan data sekunder. Penelitian dilakukan menggunakan alat analisis berupa pemetaan rantai nilai,  matriks IFE (Internal Factor Evaluation dan EFE (External Factor Evaluation, SWOT, dan QSPM (Quantitative

  11. Dormancia y tratamientos pregerminativos en las semillas de Leucaena spp. cosechadas en suelo ácido Dormancy and pregerminative treatments in Leucaena spp. seeds harvested on acid soil

    Directory of Open Access Journals (Sweden)

    Yolanda González

    2009-12-01

    Full Text Available Se desarrolló un experimento con semillas de accesiones de Leucaena spp. cosechadas en suelo ácido y almacenadas bajo condiciones ambientales, para determinar el período e intensidad de la dormancia y su ruptura. Para ello se empleó un diseño de clasificación simple y cuatro réplicas, con los siguientes tratamientos: 1 semilla sin tratar (control; 2 corte de cubierta; y 3 agua a 80°C durante 2 minutos. En cada una de las accesiones hubo diferencias significativas entre los tratamientos aplicados y los mayores valores de germinación se encontraron con el corte de cubierta. Todas las accesiones mostraron estado dormático (entre 10 y 98%, el cual se apreció en la germinación del control y en la viabilidad con el corte de cubierta. Los mayores valores de dormancia en las semillas cosechadas en el 2006 se detectaron en L. macrophylla CIAT 17233, L. macrophylla CIAT 17231, L. diversifolia CIAT 17270 y L. leucocephala IH-1140, cuya dureza fue de 98,0; 97,0; 80,0 y 74,7%, respectivamente; en las cosechadas en el 2003 y 2004, los porcentajes de dureza fueron de 89,0; 69,0 y 60,0% para L. lanceolata CIAT 17252, L. macrophylla CIAT 17238 y L. leucocephala cv. K-67, respectivamente. Se concluye que las semillas de las accesiones presentaron dormancia poscosecha, que varió entre 10 y 98%. Además la escarificación térmica provocó su ruptura, por lo que se recomienda aplicar este procedimiento antes de la siembra a las semillas de Leucaena spp. que se cosechen en suelo ácido de Cascajal.A trial was conducted with seeds of Leucaena spp accessions harvested on acid soil and stored under ambient conditions, in order to determine the period and intensity of dormancy and its breaking. For that purpose a simple classification design and four replications were used, with the following treatments: 1 untreated seed (control; 2 coat cut; and 3 water at 80ºC for 2 minutes. In each one of the accessions there were significant differences among the

  12. Leptospira spp. in commensal rodents, Beijing, China.

    Science.gov (United States)

    Wang, Chengmin; He, Hongxuan

    2013-04-01

    Leptospirosis is a widely distributed zoonosis, and rats (Rattus spp.) are its most common source. We found antibodies to leptospires in 115 (30.2%) of 381 commensal rodents from Beijing, China. Commensal rodents might represent a potential source for human and pet leptospirosis in urban environments.

  13. SPAWNING MIGRATION OF LABEOBARBUS SPP. (PISCES ...

    African Journals Online (AJOL)

    Pairwise comparison of the Labeobarbus spp. showed temporal segregation in all sampling months, except L. intermedius and L. brevicephalus that did not show temporal segregation with L. nedgia. The best management option to protect these species is closed season that should be strictly implemented during the ...

  14. EKOLOGI Anopheles spp. DI KABUPATEN LOMBOK TENGAH

    Directory of Open Access Journals (Sweden)

    Majematang Mading

    2014-07-01

    Full Text Available Malaria remains a public health problem in West Nusa Tenggara Province. Central Lombok District is one of the areas with high case of malaria. Annual Malaria Incidence (AMI was increased from 5.9 ‰ in 2006, 6.7‰ up to 8.1‰ in 2008. The objective of the study is to describe the ecological condition of Anopheles spp. through observation, measurement of environmental variables, larvae and adult collection. This research was an observational research with cross-sectional study. The population of this study is all mosquitos and breeding habitats of Anopheles spp. that exist in the research location. Ecological observations carried out on anopheles breeding habitats including acidity, salinity, shaded places and aquatic biota. Air temperature and humidity measured at the adult mosquitoes trapping sites. The result showed that pH values of water is around 9.00, salinity in the breeding habitats around 14 ppm, and water biota (i.e. moss, grass, shrimps, fishes, tadpoles and crabs surrounded by bushes with larvae density 0,1-28,8 each dipping. The air measurement at the time was between 23°-27° Celsius and 65%-84% humidity. This research concludes that ecology and environmental conditions were supporting the development of larvae and adult mosquito of Anopheles spp.Keywords: ecology, Anopheles spp., Central Lombok

  15. Toward in vitro fertilization in Brachiaria spp.

    NARCIS (Netherlands)

    Dusi, D.; Alves, E.R.; Willemse, M.T.M.; Falcao, R.; Valle, do C.B.; Carneiro, V.T.C.

    2010-01-01

    Brachiaria are forage grasses widely cultivated in tropical areas. In vitro pollination was applied to accessions of Brachiaria spp. by placing pollen of non-dehiscent anthers on a solid medium near isolated ovaries. Viability and in vitro germination were tested in order to establish good

  16. Occurrence of Cronobacter spp. in retail foods

    Czech Academy of Sciences Publication Activity Database

    Hochel, I.; Růžičková, H.; Krásný, Lukáš; Demnerová, K.

    2012-01-01

    Roč. 112, č. 6 (2012), s. 1257-1265 ISSN 1364-5072 Grant - others:GA ČR(CZ) GAP503/10/0664 Institutional research plan: CEZ:AV0Z50200510 Keywords : biotype * antibiotic resistance * Cronobacter spp Subject RIV: CE - Biochemistry Impact factor: 2.196, year: 2012

  17. Regeneration in selected Cucurbita spp. germplasm

    OpenAIRE

    Gisbert Domenech, Maria Carmen; Picó Sirvent, María Belén N:2949; Nuez Viñals, Fernando

    2011-01-01

    Gisbert Domenech, MC.; Picó Sirvent, MBN.; Nuez Viñals, F. (2011). Regeneration in selected Cucurbita spp. germplasm. Report- Cucurbit Genetics Cooperative. 33-34:53-54. http://hdl.handle.net/10251/62926 Senia 53 54 33-34

  18. Identification and pathogenicity assessment of Fusarium spp ...

    African Journals Online (AJOL)

    Durum wheat is the major cereal crop cultivated in Tunisia; covering over 40% of the cereal growing areas. Durum wheat production remains below expectation due to its low productivity that is attributed to the chronically abiotic and biotic stresses. Fusarium head blight (FHB) caused by Fusarium spp. has become an ...

  19. Induced systemic resistance by fluorescent Pseudomonas spp.

    NARCIS (Netherlands)

    Bakker, P.A.H.M.; Pieterse, C.M.J.; Loon, L.C. van

    2007-01-01

    Fluorescent Pseudomonas spp. have been studied for decades for their plant growth-promoting effects through effective suppression of soilborne plant diseases. The modes of action that play a role in disease suppression by these bacteria include siderophore-mediated competition for iron, antibiosis,

  20. Suppressive effects of metabolites from Photorhabdus spp. and Xenorhabdus spp. on phytopathogens of peach and pecan

    Science.gov (United States)

    Our objective was to determine the suppressive abilities of bacterial metabolites derived from Photorhabdus and Xenorhabdus spp. on Glomerella cingulata, Phomopsis sp., Phytophthora cactorum, and Fusicladosporium effusum, which are fungal or oomycete pathogens of pecan, and Monilinia fructicola, a f...

  1. Biological control of saltcedar (Tamarix spp.) by saltcedar leaf beetles (Diorhabda spp.): effects on small mammals

    Science.gov (United States)

    The spread of introduced saltcedar (Tamarix spp.) throughout many riparian systems across the western United States motivated the introduction of biological control agents that are specific to saltcedar, saltcedar leaf beetles (Diorhabda carinulata, D. elongata; Chrysomelidae). I monitored small mam...

  2. 21 CFR 866.3350 - Leptospira spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Leptospira spp. serological reagents. 866.3350... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3350 Leptospira spp. serological reagents. (a) Identification. Leptospira spp. serological reagents are devices that...

  3. 21 CFR 866.3415 - Pseudomonas spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Pseudomonas spp. serological reagents. 866.3415... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3415 Pseudomonas spp. serological reagents. (a) Identification. Pseudomonas spp. serological reagents are devices that...

  4. 21 CFR 866.3550 - Salmonella spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Salmonella spp. serological reagents. 866.3550... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3550 Salmonella spp. serological reagents. (a) Identification. Salmonella spp. serological reagents are devices that...

  5. 21 CFR 866.3740 - Streptococcus spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Streptococcus spp. serological reagents. 866.3740... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3740 Streptococcus spp. serological reagents. (a) Identification. Streptococcus spp. serological reagents are devices...

  6. Vibrio spp. and Salmonella spp., presence and susceptibility in crabs Ucides cordatus Vibrio spp. e Salmonella spp. em caranguejos, Ucides cordatus

    Directory of Open Access Journals (Sweden)

    Regine H.S.F. Vieira

    2004-08-01

    Full Text Available The presence of Vibrio spp. and Salmonella spp. in crabs marketed at the Bezerra de Menezes Ave., Fortaleza, State of Ceará, Brazil, was assessed between February and May, 2003. The number of individuals sampled in each one of the fifteen weekly samplings ranged between four and eight. Seven strains of Salmonella, from four different samplings, were identified, being five of them identified as serotype S. Senftenberg and two as S. Poona. All strains of Salmonella were sensitive to the tested anti-microbial drugs, with the exception of tetracycline and nalidixic acid, for which an intermediary sensibility was found. The MPN's for Vibrio ranged between 110/g and 110,000/g. Of the forty five Vibrio strains isolated from the crab samples, only 10 were identified up to the species level: two V. alginolyticus and eight V. parahaemolyticus. Bacteria belonging to the Enterobacteriaceae and Pseudomonaceae families were also identified, namely Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Pantoea agglomerans and Pseudomonas aeruginosa. The proper cooking of the animals is recommended in order to avoid problems for the consumers of this crustacean.Foram pesquisadas a presença de Vibrio spp. e de Salmonella spp. em caranguejos comercializados na Av. Bezerra de Menezes, Fortaleza, Ceará, Brasil, no período entre fevereiro e maio de 2003. O número de indivíduos em cada, das quinze coletas realizadas, semanalmente, variava entre quatro e oito dependendo do tamanho dos animais, totalizando um número de 90 (noventa animais examinados. Foram identificadas sete cepas de Salmonella spp. provenientes de quatro coletas: cinco foram identificadas como sorovar S. Senftenberg e duas como S. Poona. Todas as cepas de Salmonella, isoladas das amostras de caranguejos, apresentaram sensibilidade aos antimicrobianos testados, com exceção de tetraciclina e ácido nalidíxico para os quais elas apresentaram uma sensibilidade intermediária. Os NMPs

  7. 3CV+2: modelo de calidad para la construcción de la vivienda 3CV+2: quality model for dwelling construction

    Directory of Open Access Journals (Sweden)

    Salvador García Rodríguez

    2008-08-01

    Full Text Available Este artículo presenta el modelo y los resultados que, desde Junio de 2005, se han alcanzado en el desarrollo del Programa Nacional de Certificación de los Procesos Constructivos de la Vivienda en base al Modelo 3cv+2. El programa se ha desarrollado con base a un convenio entre el Tecnológico de Monterrey y la Cámara Nacional de la Industria de Desarrollo y Promoción de Vivienda. Actualmente se encuentran voluntariamente en el programa 52 empresas inmobiliarias de diferentes estados de la República Mexicana. El modelo de calidad 3cv+2 es una metodología que busca establecer en los niveles operativos de la construcción criterios que permitan reducir la variabilidad del proceso de construcción, y además permita de manera sistemática, y en un proceso de mejora continua, evaluar el desempeño de calidad en proyectos de construcción de vivienda. El modelo 3cv+2 define en su nombre el alcance del mismo, 3 criterios de calidad para la vivienda (insumos, proceso y producto, apoyado por 2 auditorías (+2, la auditoría interna de la propia empresa, mas la auditoría externa hecha por el Tecnológico de Monterrey. El modelo 3cv+2 busca garantizar que los insumos utilizados en la construcción de las viviendas sean de calidad superior, y que los procesos de ejecución posean características de calidad, productividad, eficiencia, racionalización, y estandarización; de tal manera que insumos y productos sean de alto valor agregado, y garanticen la calidad especificada en las etapas parciales y en la vivienda terminada.This paper presents the model and the results that have been achieved during the development of the National Quality Certification Program based on the model 3cv +2 from June 2005. This program has been developed based on an agreement between the Monterrey Tech and the National Chamber of Housing Industry. Nowadays, the program has 52 voluntary real estate companies from different states of Mexico. The 3cv+2 quality model is a

  8. Presence of Staphylococcus spp. and Candida spp. in the human oral cavity

    Directory of Open Access Journals (Sweden)

    Martins Clélia Aparecida de Paiva

    2002-01-01

    Full Text Available The presence of yeasts and staphylococci in the oral cavity is important because they can act as supplementary microbiota and in certain situations can cause oral or systemic diseases. The aim of this work was to study the prevalence of Candida spp. and Staphylococcus spp. in the human oral cavity. Oral rinses were collected from sixty-eight individuals according to the technique described by Samaranayake and MacFarlane and then cultured on Sabouraud medium supplemented with chloramphenicol and Baird-Parker agar. After the incubation period, the microorganisms were isolated and identified through biochemical tests. The data obtained were statistically analysed by ANOVA. Candida spp. were isolated from 61.76% of the examined individuals and C. albicans was the more frequently isolated specie. Staphylococcus spp. were isolated from 95.60% of the individuals and 41 strains were coagulase negative (63%. Among the coagulase positive strains, nine were S. aureus, 11 S. hyicus and 4 S. schleiferi subspecie coagulans. No correlation was observed between the counts (cfu of the isolated Candida spp. and Staphylococcus spp.

  9. Segmentation of kidney using C-V model and anatomy priors

    Science.gov (United States)

    Lu, Jinghua; Chen, Jie; Zhang, Juan; Yang, Wenjia

    2007-12-01

    This paper presents an approach for kidney segmentation on abdominal CT images as the first step of a virtual reality surgery system. Segmentation for medical images is often challenging because of the objects' complicated anatomical structures, various gray levels, and unclear edges. A coarse to fine approach has been applied in the kidney segmentation using Chan-Vese model (C-V model) and anatomy prior knowledge. In pre-processing stage, the candidate kidney regions are located. Then C-V model formulated by level set method is applied in these smaller ROI, which can reduce the calculation complexity to a certain extent. At last, after some mathematical morphology procedures, the specified kidney structures have been extracted interactively with prior knowledge. The satisfying results on abdominal CT series show that the proposed approach keeps all the advantages of C-V model and overcome its disadvantages.

  10. Immobilization of enzymatic extracts of Portulaca oleracea cv. roots for oxidizing aqueous bisphenol A.

    Science.gov (United States)

    Matsushima, Kazuki; Kaneda, Hirokazu; Harada, Kazuo; Matsuura, Hideyuki; Hirata, Kazumasa

    2015-05-01

    Water pollution from the release of industrial wastewater is a serious problem for almost every industry. Enzymes from portulaca, Portulaca oleracea cv., have been investigated for their ability to degrade bisphenol A (BPA), one of the well-known estrogenic pollutants. Enzymatic crude extracts from P. oleracea cv. roots were immobilized on aminopropyl-modified glass beads. They maintained BPA metabolic activity over a broad range of pH values and temperatures. The immobilized enzyme was reusable with more than 50 % of its initial activity retained after 12 batch reactions and no loss of activity after storage for 1 month at -30 °C. Thus, the immobilization of extracts from P. oleracea cv. roots is a useful method for removing BPA from industrial wastewater.

  11. Khatyrka, a new CV3 find from the Koryak Mountains, Eastern Russia

    Science.gov (United States)

    MacPherson, Glenn J.; Andronicos, Christopher L.; Bindi, Luca; Distler, Vadim V.; Eddy, Michael P.; Eiler, John M.; Guan, Yunbin; Hollister, Lincoln S.; Kostin, Alexander; Kryachko, Valery; Steinhardt, William M.; Yudovskaya, Marina; Steinhardt, Paul J.

    2013-08-01

    A new meteorite find, named Khatyrka, was recovered from eastern Siberia as a result of a search for naturally occurring quasicrystals. The meteorite occurs as clastic grains within postglacial clay-rich layers along the banks of a small stream in the Koryak Mountains, Chukotka Autonomous Okrug of far eastern Russia. Some of the grains are clearly chondritic and contain Type IA porphyritic olivine chondrules enclosed in matrices that have the characteristic platy olivine texture, matrix olivine composition, and mineralogy (olivine, pentlandite, nickel-rich iron-nickel metal, nepheline, and calcic pyroxene [diopside-hedenbergite solid solution]) of oxidized-subgroup CV3 chondrites. A few grains are fine-grained spinel-rich calcium-aluminum-rich inclusions with mineral oxygen isotopic compositions again typical of such objects in CV3 chondrites. The chondritic and CAI grains contain small fragments of metallic copper-aluminum-iron alloys that include the quasicrystalline phase icosahedrite. One grain is an achondritic intergrowth of Cu-Al metal alloys and forsteritic olivine ± diopsidic pyroxene, both of which have meteoritic (CV3-like) oxygen isotopic compositions. Finally, some grains consist almost entirely of metallic alloys of aluminum + copper ± iron. The Cu-Al-Fe metal alloys and the alloy-bearing achondrite clast are interpreted to be an accretionary component of what otherwise is a fairly normal CV3 (oxidized) chondrite. This association of CV3 chondritic grains with metallic copper-aluminum alloys makes Khatyrka a unique meteorite, perhaps best described as a complex CV3 (ox) breccia.

  12. Detoxification of microcystin-LR in water by Portulaca oleracea cv.

    Science.gov (United States)

    Isobe, Takatoshi; Okuhata, Hiroshi; Miyasaka, Hitoshi; Jeon, Bong-Seok; Park, Ho-Dong

    2014-03-01

    Microcystin-LR (0.02 μg/ml) in the hydroculture medium of Portulaca oleracea cv., became below the detection level (<0.0001 μg/ml) by HPLC analysis after 7 days. The toxicity of microcystin estimated with protein phosphatase inhibition assay, however, remained at 37% of the initial level, indicating that microcystin-LR was transformed by P. oleracea cv. into unknown compound(s) of lower toxicity. Copyright © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  13. The Role of Malassezia spp. in Atopic Dermatitis

    Science.gov (United States)

    Glatz, Martin; Bosshard, Philipp P.; Hoetzenecker, Wolfram; Schmid-Grendelmeier, Peter

    2015-01-01

    Malassezia spp. is a genus of lipophilic yeasts and comprises the most common fungi on healthy human skin. Despite its role as a commensal on healthy human skin, Malassezia spp. is attributed a pathogenic role in atopic dermatitis. The mechanisms by which Malassezia spp. may contribute to the pathogenesis of atopic dermatitis are not fully understood. Here, we review the latest findings on the pathogenetic role of Malassezia spp. in atopic dermatitis (AD). For example, Malassezia spp. produces a variety of immunogenic proteins that elicit the production of specific IgE antibodies and may induce the release of pro-inflammatory cytokines. In addition, Malassezia spp. induces auto-reactive T cells that cross-react between fungal proteins and their human counterparts. These mechanisms contribute to skin inflammation in atopic dermatitis and therefore influence the course of this disorder. Finally, we discuss the possible benefit of an anti-Malassezia spp. treatment in patients with atopic dermatitis. PMID:26239555

  14. CTX-M extended-spectrum β-lactamase-producing Klebsiella spp, Salmonella spp, Shigella spp and Escherichia coli isolates in Iranian hospitals.

    Science.gov (United States)

    Bialvaei, Abed Zahedi; Kafil, Hossein Samadi; Asgharzadeh, Mohammad; Aghazadeh, Mohammad; Yousefi, Mehdi

    2016-01-01

    This study was conducted in Iran in order to assess the distribution of CTX-M type ESBLs producing Enterobacteriaceae. From January 2012 to December 2013, totally 198 E. coli, 139 Klebsiella spp, 54 Salmonella spp and 52 Shigella spp from seven hospitals of six provinces in Iran were screened for resistance to extended-spectrum cephalosporins. After identification and susceptibility testing, isolates presenting multiple-drug resistance (MDR) were evaluated for ESBL production by the disk combination method and by Etest using (cefotaxime and cefotaxime plus clavulanic acid). All isolates were also screened for blaCTX-M using conventional PCR. A total of 42.92%, 33.81%, 14.81% and 7.69% of the E. coli, Klebsiella spp, Salmonella spp and Shigella spp isolates were MDR, respectively. The presence of CTX-M enzyme among ESBL-producing isolates was 85.18%, 77.7%, 50%, and 66.7%, in E. coli, Klebsiella spp, Salmonella spp and Shigella spp respectively. The overall presence of CTX-M genes in Enterobacteriaceae was 15.4% and among the resistant isolates was 47.6%. This study indicated that resistance to β-lactams mediated by CTX-M enzymes in Iran had similar pattern as in other parts of the world. In order to control the spread of resistance, comprehensive studies and programs are needed. Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

  15. PREVALENCE OF BABESIA SPP., EHRLICHIA SPP., AND TICK INFESTATIONS IN OKLAHOMA BLACK BEARS (URSUS AMERICANUS).

    Science.gov (United States)

    Skinner, Delaina; Mitcham, Jessica R; Starkey, Lindsay A; Noden, Bruce H; Fairbanks, W Sue; Little, Susan E

    2017-10-01

    American black bears (Ursus americanus) are commonly infested with ticks throughout their range, but there are few surveys for tick-borne disease agents in bears. To characterize tick infestations and determine the prevalence of current infection with Babesia spp. and past or current infection with Ehrlichia spp. in newly re-established populations of black bears in east central and southeastern Oklahoma, US, we identified adult (n=1,048) and immature (n=107) ticks recovered from bears (n=62). We evaluated serum and whole blood samples from a subset (n=49) for antibodies reactive to, and characteristic DNA fragments of, Ehrlichia spp., as well as characteristic DNA fragments of Babesia spp. Amblyomma americanum, the most common tick identified, was found on a majority (56/62; 90%) of bears and accounted for 697/1,048 (66.5%) of all ticks recovered. Other ticks included Dermacentor variabilis (338/1,048; 32.3%) from 36 bears, Amblyomma maculatum (9/1,048; 0.9%) from three bears, and Ixodes scapularis (4/1,048; 0.4%) from three bears. Antibodies reactive to Ehrlichia spp. were detected in every bear tested (49/49; 100%); maximum inverse titers to Ehrlichia chaffeensis ranged from 64-4,096 (geometric mean titer 1,525). However, PCR failed to identify active infection with E. chaffeensis, Ehrlichia ewingii, or an Ehrlichia ruminantium-like agent. Infection with Babesia spp. was detected by PCR in 3/49 (6%) bears. Together these data confirm that tick infestations and infection with tick-borne disease agents are common in bears in the southern US. The significance of these infestations and infections to the health of bears, if any, and the identity of the Ehrlichia spp. responsible for the antibody reactivity seen, warrant further evaluation.

  16. Easy storage strategies for Sporothrix spp. strains.

    Science.gov (United States)

    Brilhante, Raimunda Sâmia Nogueira; Silva, Natalya Fechine; Lima, Rita Amanda Chaves de; Caetano, Érica Pacheco; Alencar, Lucas Pereira de; Castelo-Branco, Débora de Souza Collares Maia; Moreira, José Luciano Bezerra; Bandeira, Silviane Praciano; Camargo, Zoilo Pires de; Rodrigues, Anderson Messias; Bandeira, Tereza de Jesus Pinheiro Gomes; Monteiro, André Jalles; Cordeiro, Rossana de Aguiar; Sidrim, José Júlio Costa; Rocha, Marcos Fábio Gadelha

    2015-04-01

    The present study evaluated the maintenance of Sporothrix spp. (6 Sporothrix brasiliensis; 6 S. schenckii; 5 S. mexicana, and 3 S. globosa) in saline at 4°C, and in 10% glycerol plus either 10% lactose or 10% sucrose, at -20°C and -80°C. Viability was assessed after 3, 6, and 9 months of storage, through the recovery of strains on potato dextrose agar and analysis of macro- and micromorphological features. Conidium quantification was performed before and after storage, at 3, 6 and 9 months. 100% viability was observed, regardless of storage conditions or time period. Storage at 4°C and at -20°C did not alter the number of conidia, but lower conidium counts were observed at -80°C. This study shows that the combination of glycerol with lactose or sucrose is effective to maintain Sporothrix spp. at freezing temperatures.

  17. Induced systemic resistance by fluorescent Pseudomonas spp.

    OpenAIRE

    Bakker, P.A.H.M.; Pieterse, C.M.J.; Loon, L.C. van

    2007-01-01

    Fluorescent Pseudomonas spp. have been studied for decades for their plant growth-promoting effects through effective suppression of soilborne plant diseases. The modes of action that play a role in disease suppression by these bacteria include siderophore-mediated competition for iron, antibiosis, production of lytic enzymes, and induced systemic resistance (ISR). The involvement of ISR is typically studied in systems in which the Pseudomonas bacteria and the pathogen are inoculated and rema...

  18. The influence of virus diseases on grape polyphenols of cv. 'Refosk'

    International Nuclear Information System (INIS)

    Tomazic, I.; Vrhovsek, U.; Korosec-Koruza, Z.

    2003-01-01

    External stimuli such as microbial infections, ultraviolet radiation, and chemical stressors can modulate the synthesis of polyphenols in the plants. Cv. 'Refosk' was used to show the influence of the GLRaV-1 and rugose wood (RW) on the polyphenols in grape. The infection shifted polyphenols from seeds to grape skins but had no impact on anthocyanins

  19. Lupin seed meal ( Lupinus albus cv. Buttercup) as a source of ...

    African Journals Online (AJOL)

    Two experiments were conducted to evaluate L. a/bus cv. Buttercup as an alternative to heated full-fat soya bean meal for early weaned piglets. In Experiment 1, the performance of piglets, fed diets with 0%,4%,8% and 12% lupins and formulated to contain equivalent amounts of digestible energy and lysine, was used to ...

  20. RADISH CV. MOKHOVSKIY IS A SOURCE OF MS- AND MF-LINES AT BREEDING FOR HETEROSIS

    Directory of Open Access Journals (Sweden)

    M. I. Fedorova

    2015-01-01

    Full Text Available Based on hybrid and inbreed population of radish cv. Mokhovskiy using CMS-ogura nine lines with 100% of male sterility and fertile maintainers were selected. Using the multiplex PCR, the presence of cytoplasm of Ogura type in ms-lines and its absence in mf-lines were confirmed.

  1. Optimized growth and plant regeneration for callus of Lilium longiflorum cv. Nellie White

    Science.gov (United States)

    The rates of growth and regeneration were compared for compact callus, friable callus, and suspension cells of Lilium longiflorum cv. Nellie White to determine the optimal culture conditions. The fresh weight was higher for compact callus induced from bulb scales cultured on Murashige and Skoog’s m...

  2. Lipid abnormalities in streptozotocin-diabetes: Amelioration by Morus indica L. cv Suguna leaves

    OpenAIRE

    Andallu, B.; Vinay Kumar, A. V.; Varadacharyulu, N. Ch.

    2009-01-01

    AIM: To observe the influence of mulberry (Morus indica L. cv Suguna) leaves on lipid abnormalities in STZ-diabetic rats. MATERIALS AND METHODS: Treatment with dried mulberry leaf powder for a period of 8 weeks in hyperglycemic and hyperlipidemic STZ-diabetic rats. RESULTS: Mulberry leaves regulated fasting blood glucose, ameliorated the abnormalities in lipid profile as indicated by significant (P

  3. Agrobacterium-mediated transformation of Easter lily (Lilium longiflorum cv. Nellie White)

    Science.gov (United States)

    Conditions were optimized for transient transformation of Lilium longiflorum cv. Nellie White using Agrobacterium tumefaciens. Bulb scale and basal meristem explants were inoculated with A. tumefaciens strain AGL1 containing the binary vector pCAMBIA 2301 which has the uidA gene that codes for ß-gl...

  4. Effect of LEDs on flower bud induction in Chrysanthemum morifolium cv. Zembla

    NARCIS (Netherlands)

    Singh, M.; Ieperen, van W.; Heuvelink, E.

    2013-01-01

    The effect of LEDs was studied to induce flower under artificial long days (LD) in Chrysanthemum morifolium cv. Zembla plants, using light emitting diodes (LED) @ PAR m-2 s-1 80% Red / 20% Blue maintained @ 100 µ mol m- 2 s- 1 using royal blue light @ 455 nm and red light @ 640 nm wavelengths and

  5. PlantCV v2: Image analysis software for high-throughput plant phenotyping.

    Science.gov (United States)

    Gehan, Malia A; Fahlgren, Noah; Abbasi, Arash; Berry, Jeffrey C; Callen, Steven T; Chavez, Leonardo; Doust, Andrew N; Feldman, Max J; Gilbert, Kerrigan B; Hodge, John G; Hoyer, J Steen; Lin, Andy; Liu, Suxing; Lizárraga, César; Lorence, Argelia; Miller, Michael; Platon, Eric; Tessman, Monica; Sax, Tony

    2017-01-01

    Systems for collecting image data in conjunction with computer vision techniques are a powerful tool for increasing the temporal resolution at which plant phenotypes can be measured non-destructively. Computational tools that are flexible and extendable are needed to address the diversity of plant phenotyping problems. We previously described the Plant Computer Vision (PlantCV) software package, which is an image processing toolkit for plant phenotyping analysis. The goal of the PlantCV project is to develop a set of modular, reusable, and repurposable tools for plant image analysis that are open-source and community-developed. Here we present the details and rationale for major developments in the second major release of PlantCV. In addition to overall improvements in the organization of the PlantCV project, new functionality includes a set of new image processing and normalization tools, support for analyzing images that include multiple plants, leaf segmentation, landmark identification tools for morphometrics, and modules for machine learning.

  6. An evaluation of Panicum maximum cv. Gatton: 1. The effect of ...

    African Journals Online (AJOL)

    Unknown

    The aim of this study was to evaluate the livestock production potential of Panicum maximum cv. Gatton ... consumed which varies according to the quantity and quality of forage on offer (Heitschmidt et al., 1990). The .... Dry matter availability differed between treatments both at the beginning and end of each grazing period.

  7. STRATEGI SEGMENTASI PASAR TERHADAP VOLUME PENJUALAN BUSANA MUSLIM PADA CV RABBANI ASYSA MAKASSAR

    OpenAIRE

    KARASE, HASNAWATI

    2014-01-01

    2014 Hasnawati Karase Haris Maupa Mukhtar Penelitian ini bertujuan untuk menganalisis strategi segmentasi pasar terhadap volume penjualan busana muslim pada CV Rabbani Asysa Makassar. Data penelitian ini diperoleh dari kuesioner (primer) mengenai segmentasi pasar Rabbani Makassar dengan mengambil sampel sebanyak 75 responden. Temuan penelitian ini menunjukkan bahwa variabel strategi segmentasi pasar yang terdiri dari segmentasi geografis, segmentasi demografis, segm...

  8. PlantCV v2: Image analysis software for high-throughput plant phenotyping

    Directory of Open Access Journals (Sweden)

    Malia A. Gehan

    2017-12-01

    Full Text Available Systems for collecting image data in conjunction with computer vision techniques are a powerful tool for increasing the temporal resolution at which plant phenotypes can be measured non-destructively. Computational tools that are flexible and extendable are needed to address the diversity of plant phenotyping problems. We previously described the Plant Computer Vision (PlantCV software package, which is an image processing toolkit for plant phenotyping analysis. The goal of the PlantCV project is to develop a set of modular, reusable, and repurposable tools for plant image analysis that are open-source and community-developed. Here we present the details and rationale for major developments in the second major release of PlantCV. In addition to overall improvements in the organization of the PlantCV project, new functionality includes a set of new image processing and normalization tools, support for analyzing images that include multiple plants, leaf segmentation, landmark identification tools for morphometrics, and modules for machine learning.

  9. In vitro propagation of Rosa hybrida L. cv. Al-Taif Rose plant | Attia ...

    African Journals Online (AJOL)

    In this study, a protocol for in vitro propagation of Rosa hybrida L. cv. Al-Taif Rose was established using nodal segments harboring axillary buds as explants. In vitro stages of shoot initiation, multiplication and elongation were performed. Explants were cultured on solid Murashige and Skoog medium (MS) supplemented ...

  10. Oxygen isotope systematics of chondrules in the Allende CV3 chondrite: High precision ion microprobe studies

    Digital Repository Service at National Institute of Oceanography (India)

    Rudraswami, N.G.; Ushikubo, T.; Nakashima, D.; Kita, N.T.

    in local dust-rich protoplanetary disk, from which the CV3 parent asteroid formed. A compilation of 225 olivine and low-Ca pyroxene isotopic data from 36 chondrules analyzed in the present study lie between carbonaceous chondrite anhydrous mineral (CCAM...

  11. NALISIS PENGAKUAN PENDAPATAN BERDASARKAN PSAK NOMOR 23 PADA CV TILZAK KABUPATEN PINRANG

    Directory of Open Access Journals (Sweden)

    ILHAM ARIFIN

    2017-05-01

    This research was conducted at CV. Tilzak Pinrang which is a business as a cog in the business of selling. Based on research that has been done, it is not all revenue recognition in accordance with IAS 23 is not dibuatkannya marked revenue balance sheet and income statement, so that the impact to the company's earnings were only included proceeds earned in one year.

  12. Morphological characters of Panicum maximum cv Gatton planted from different source of sapling

    Directory of Open Access Journals (Sweden)

    Fanindi A

    2014-03-01

    Full Text Available Morphological characters and agronomic information of Panicum maximum cv Gatton is required in forage crop breeding activities. Therefore a research was conducted to determine the morphology and characteristic of generative phase of Panicum maximum cv Gatton. The study was conducted in greenhouses, using a 40 cm diameter pot. The planting medium was soil from Ciawi (Latosol. Research design was Completely Randomized Design, with 10 replications. Treatments used were 2 types of planting material, 1 derived from the pols (vegetative and 2 seeds. Plants used were Panicum maximum cv Gatton that have been collected and evaluated at the IRIAP, Ciawi. Variables measured were plant morphology, reproductive traits, seed production per panicle and seed quality. Results showes that morphological characters of cv Gatton derived from seed and pols was not significantly different (p > 0.05, whereas the flowering time and the time of seeds mature after cutting derived from the seeds was faster (p < 0.05 than cultivars derived from pols. While the production of seed derived from the seeds was higher (p < 0.05 when compared to that derived from the pols.

  13. Sales Systems on Madju Cv.menara Using Visual Basic 6.0

    OpenAIRE

    Rohmat Rohmat

    2006-01-01

    Sales System is a system that can support operational CV.Menara Madju in improvingservice to customers. System that contains information about products that complete withits specifications include the price. Scientific writing illustrates how to create a designSales System using Visual Basic 6.0.Scientific Writing Hopefully this can help to develop a more Interactive Sales Systemsand Innovative.

  14. Evaluation of possible equivalent circuits for the description of the CV characteristics of heavily irradiated Si diodes

    Science.gov (United States)

    Campbell, D.; Chilingarov, A.; Sloan, T.

    2005-10-01

    Different equivalent circuit diagrams are evaluated for the representation of the CV characteristics, measured with standard equipment, for a typical Si diode after heavy irradiation. A general approach is developed and several minimal models are analysed. A possible mechanism is proposed for the frequency dependence of the depletion voltage extracted from the CV measurements.

  15. Efect of intercellular extracts from banana inoculated leaves with Mycosphaerella fijiensis Morelet, on chloroplast electronic transport of Grande naine (AAA cv.

    Directory of Open Access Journals (Sweden)

    Michel Leiva-Mora

    2003-01-01

    Full Text Available Some foliar pathogens colonize intercellular spaces of damage tissues during infection process, mediated by toxins production and diffusion to kill adjacent healthy cells. Due to the absence of reliable bioassays, the physiologic effects of several phytotoxins are still ignored on cellular membranous systems of the affected cells. In the present work it was extracted the intercellular content from not inoculated and inoculated banana leaves with different Mycosphaerella fijiensis strains. Their effects on chloroplasts of Grande naine cv were evaluated by the absorbance evolution (595 nm of Hill reactive (DCPIP, mixture with 810 ì l of chloroplasts suspension and 99 ì l of the intercellular contents. The electronic exchange on chloroplasts suspension was inhibited by intercellular contents of inoculated leaves. The intercellular contents from leaves inoculated with I1 (high virulence strain had a major inhibiter effect respect to leaves inoculates with G1 strain (low virulence, showing a correspondence between the inhibiter effect of intercellular contents and the affection levels of affected tissues. The procedures used in this work will let to make studies concerned with Mycosphaerella fijiensis-Musa spp interactions and the future breeding programs. Key words: banana breeding, black Sigatoka, host pathogen interaction, physiological bioassays

  16. ANALISIS KEBUTUHAN TENAGA KERJA RESERVASI TERHADAP PERENCANAAN PENGEMBANGAN SUMBER DAYA MANUSIA PADA CV. CAN TOUR & TRAVEL

    Directory of Open Access Journals (Sweden)

    Maya Christina

    2017-01-01

    Full Text Available Employment remains the fundamental probel faced by many countries including Indonesia. One of the efforts the Government of Indonesia in extending employment opportunities is to develop the tourism sector. Bali is one of the potential tourist destinastions and it needs to be marketed. Cooperation between the Travel Bureau with the provider of the accommodation or hotel is one of the ways the potential market and the most instrumental in the development of the company, namely human resources required or called by labor. CV. CAN Tour and Travel is one of the tourist industry in Bali trip with a number of guest handling that quite a lot. This research aims to know the availability of labor needs resevartion in order CV. CAN Tour and Travel development and also to find out how the workforce qualification is required. Determination technique using the key informant that was labor reservation CV. CAN Tour and Travel, while the base of the informant was CV. CAN Tour and Travel Manager. Data analysis technique used is the analysis of workload and workforce needs analysis. The research results obtained indicate that shortage of time working as many as 210 minutes. So the manpower needed by CV. CAN Tour and Travel is two people with the fact that there is only 1 nowaday. It can be seen from the burden of the work done in a day’s work and the workforce absences. So labor needs on the Reservation Department is supposed to be 3 people, with 2 staff 1 reservations and ticketing staff. It was necessary increase manpower in the Labour Departmen’s Reservation in the reservation and also training to develop the ability to work.

  17. Fermentability of an enzymatically modified solubilised potato polysaccharide (SPP)

    DEFF Research Database (Denmark)

    Olesen, M.; Gudmund-Høyer, E.; Norsker, Merete

    1998-01-01

    : Seven healthy volunteers ingested in random order on seven different days: 20 g SPP; bread made of 180 g wheat flour served with 20 g raw SPP; bread baked of 180 g wheat flour and 20 g SPP; bread made from 180 g what flour; 20 g lactulose; 20 g oat bran; and 20 g wheat bran. The hydrogen breath test...... was used to evaluate oro-coecal transit time (OCTT) and fermentation. RESULTS: Fermentation of SPP yielded a measurable increase in end-expiratory H2. The total incremental increase in end expiratory H2 due to SPP was unaffected of whether SPP was served alone, as the raw flour served with bread, or baked...... into bread. The OCTT for raw SPP was significantly delayed compared to lactulose (P = 0.01). The OCTT for SPP baked into bread was significantly delayed compared to raw SPP (P = 0.01), indicating that SPP may be used as a marker of oro-coecal transit time for as well the fluid phase as the solid phase...

  18. INK128 Exhibits Synergy with Azoles against Exophiala spp. and Fusarium spp.

    Science.gov (United States)

    Gao, Lujuan; Sun, Yi; He, Chengyan; Li, Ming; Zeng, Tongxiang; Lu, Qiaoyun

    2016-01-01

    Infections of Exophiala spp. and Fusarium spp. are often chronic and recalcitrant. Systemic disseminations, which mostly occur in immunocompromised patients, are often refractory to available antifungal therapies. The conserved target of rapamycin (TOR) orchestrates cell growth and proliferation in response to nutrients and growth factors, which are important for pathogenicity and virulence. INK128 is a second-generation ATP-competitive TOR inhibitor, which binds the TOR catalytic domain and selectively inhibits TOR. In the present study, we investigated the in vitro activities of INK128 alone and the interactions of INK128 with conventional antifungal drugs including itraconazole, voriconazole, posaconazole, and amphotericin B against 18 strains of Exophiala spp. and 10 strains of Fusarium spp. via broth microdilution checkerboard technique system adapted from Clinical and Laboratory Standards Institute broth microdilution method M38-A2. INK128 alone was inactive against all isolates tested. However, favorable synergistic effects between INK128 and voriconazole were observed in 61% Exophiala strains and 60% Fusarium strains, despite Fusarium strains exhibited high MIC values (4-8 μg/ml) against voriconazole. In addition, synergistic effects of INK128/itraconazole were shown in 33% Exophiala strains and 30% Fusarium strains, while synergy of INK128/posaconazole were observed in 28% Exophiala strains and 30% Fusarium strains. The effective working ranges of INK128 were 0.125-2 μg/ml and 1-4 μg/ml against Exophiala isolates and Fusarium isolates, respectively. No synergistic effect was observed when INK128 was combined with amphotericin B. No antagonism was observed in all combinations. In conclusion, INK128 could enhance the in vitro antifungal activity of voriconazole, itraconazole and posaconazole against Exophiala spp. and Fusarium spp., suggesting that azoles, especially voriconazole, combined with TOR kinase inhibitor might provide a potential strategy to

  19. A survey of Babesia spp. and Hepatozoon spp. in wild canids in Israel.

    Science.gov (United States)

    Margalit Levi, Maayan; Nachum-Biala, Yaarit; King, Roni; Baneth, Gad

    2018-03-20

    Babesia spp. and Hepatozoon spp. are apicomplexan parasites that infect a variety of animals, including canids. Their life-cycle includes an invertebrate hematophagous vector as a definitive host and vertebrates as intermediate hosts. The aims of this study were to investigate the prevalence and risk factors for Babesia spp. and Hepatozoon spp. infections in wild golden jackals (Canis aureus) and red foxes (Vulpes vulpes) in Israel and to compare spleen with blood sample polymerase chain reaction (PCR) for the detection of infection. Blood and spleen samples from 109 golden jackals and 21 red foxes were tested by PCR for the detection of Babesia spp. and Hepatozoon spp. using primers for the 18S ribosomal (r) RNA gene. Hepatozoon canis was detected in 50/109 (46%) of the jackals and 9/21 (43%) of the foxes. "Babesia vulpes" (the Babesia microti-like piroplasm) was detected in 4/21 (19%) of the foxes and in none of the jackals. A previously unknown genotype termed Babesia sp. MML related to Babesia lengau (96-97% identity) was detected in 1/109 (1%) of the jackals and 4/21 (19%) of the foxes. Further characterization of this genotype carried out by PCR of the rRNA internal transcribed spacer 2 (ITS2) indicated that it had only 87% identity with the B. lengau ITS2. Sex (male or female), age (juvenile or adult) and geographic zone (North, Central or South Israel) were not found to be significant risk factors for these protozoan infections. The prevalence of "B. vulpes" and Babesia sp. MML infections was significantly higher in foxes compared to jackals (χ 2  = 15.65, df = 1, P Babesia related to B. lengau from Africa.

  20. Existence of Cryptosporidium spp. oocysts and Giardia spp. cysts in well water in Nineveh governorate

    OpenAIRE

    R. G. Mohammad

    2011-01-01

    The study included examination of 110 water samples from well distributed in Mosul city and few towns and villages around it from May 2009 to March 2010 for detection of Cryptosporidium spp oocysts and Giardia spp cysts in well water. The results revealed that the prevalence of Cryptosporidium oocysts was 16.36% while the prevalence of Giardia cysts was 12.72%. The percentages of prevalence with Cryptosporidium and Giardia were in high rate in Bartilla and some villages around it 20% for Cryp...

  1. Quantification of viable but nonculturable Salmonella spp. and Shigella spp. during sludge anaerobic digestion and their reactivation during cake storage.

    Science.gov (United States)

    Fu, B; Jiang, Q; Liu, H-B; Liu, H

    2015-10-01

    The presence of viable but nonculturable (VBNC) bacterial pathogens which often fail to be detected by cultivation and can regain the cultivability if the living conditions improve were reported. The objective of this study was to determine the occurrence of VBNC Salmonella spp. and Shigella spp. in the biosolids during anaerobic digestion and its reactivation during the cake storage. The occurrence of VBNC Salmonella spp. and Shigella spp. during mesophilic, temperature-phased, thermophilic anaerobic digestion of sewage sludge and the subsequent storage were studied by RT-qPCR and most probable number (MPN) method. The VBNC incidence of Salmonella spp. and Shigella spp. during thermophilic digestion was four orders of magnitude higher than those of mesophilic digestion. Accordingly, higher resuscitation ratio of VBNC pathogens was also achieved in thermophilic digested sludge. As a result, the culturable Salmonella typhimurium contents in thermophilic digested sludge after cake storage were two orders of magnitude higher than mesophilic digestion. Both quantitative PCR and reverse transcription quantitative PCR assay results showed the two bacterial counting numbers remained stable throughout the cake storage. The results indicate that the increase in the culturable Salmonella spp. and Shigella spp. after centrifugal dewatering was attributed to the resuscitation from the VBNC state to the culturable state. Thermophilic anaerobic digestion mainly induced Salmonella spp. and Shigella spp. into VBNC state rather than killed them, suggesting that the biological safety of sewage sludge by temperature-phased anaerobic digestion should be carefully assessed. © 2015 The Society for Applied Microbiology.

  2. Pathogenic Leptospira spp. in bats: Molecular investigation in Southern Brazil.

    Science.gov (United States)

    Mayer, Fabiana Quoos; Dos Reis, Emily Marques; Bezerra, André Vinícius Andrade; Cerva, Cristine; Rosa, Júlio; Cibulski, Samuel Paulo; Lima, Francisco Esmaile Sales; Pacheco, Susi Missel; Rodrigues, Rogério Oliveira

    2017-06-01

    The present study aimed to investigate the frequency of pathogenic Leptospira spp. in Brazilian bats and to determine possible risk factors associated to it. Ninety two bats of 12 species were evaluated. Whole genomic DNA from kidneys was extracted and real-time PCR specific to pathogenic Leptospira spp. was applied. Association between the frequency of specimens positive for Leptospira spp. and sex, age, bat species or family, season of collection, geographic localization and feeding habits was evaluated. The results showed that 39.13% of analyzed bats were found positive for Leptospira spp. Nine bat species had at least one positive result. There was no association among the evaluated variables and frequency of pathogenic Leptospira spp. Although the limitations due to lack of Leptospira spp. isolation, leptospiral carriage was demonstrated in bats of different species from southern Brazil, which reinforces the need for surveillance of infectious agents in wild animals. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Contamination by Salmonella spp., Campylobacter spp. and Listeria spp. of most popular chicken- and pork-sausages sold in Reunion Island.

    Science.gov (United States)

    Trimoulinard, A; Beral, M; Henry, I; Atiana, L; Porphyre, V; Tessier, C; Leclercq, A; Cardinale, E

    2017-06-05

    One of the most popular meat products of the local "cuisine" is sausage composed with 100% chicken or 100% pork. In this study, we aimed to determine the presence of Salmonella spp., Campylobacter spp. and Listeria spp. in chicken- and pork-sausages, quantify Salmonella spp. population and identify the factors that could be associated with contamination in the outlets. Two hundred and three batches of pork and chicken sausages were randomly collected from 67 local outlets (supermarkets, groceries and butcher shops). Salmonella spp. was detected in 11.8% (95% confidence interval (CI): [10.0; 13.5]) of samples, Campylobacter spp. in 1.5% [0.7; 4.2] and Listeria monocytogenes in 5.9% [4.4; 7.3]. Most probable number of Salmonella spp. varied between 6cfu per gram to 320cfu per gram. Salmonella serotypes isolated from pork and chicken sausages were S. Typhimurium (45.8%), S. London (20.8%), S. Derby (16.7%), S. Newport (8.33%), S. Blockley (4.2%) and S. Weltevreden (4.17%). Using a logistic (mixed-effect) regression model, we found that Salmonella spp. contamination was positively associated with sausages sold in papers or plastic bags and no control of rodents. Chicken sausages were associated with a decreasing risk of Salmonella contamination. Listeria monocytogenes contamination was positively associated with the presence of fresh rodent droppings in the outlet and negatively when the staff was cleaning regularly their hands with soap and water or water only. All the sampled outlets of Reunion Island were not equivalent in terms of food safety measures. Increasing awareness of these traders remains a cornerstone to limit the presence of Salmonella spp. and Listeria spp. in sausages, particularly in a tropical context (high temperature and humidity). Copyright © 2017 Elsevier B.V. All rights reserved.

  4. Chemical Components and Cardiovascular Activities of Valeriana spp.

    Science.gov (United States)

    Chen, Heng-Wen; Wei, Ben-Jun; He, Xuan-Hui; Liu, Yan; Wang, Jie

    2015-01-01

    Valeriana spp. is a flowering plant that is well known for its essential oils, iridoid compounds such as monoterpenes and sesquiterpenes, flavonoids, alkaloids, amino acids, and lignanoids. Valeriana spp. exhibits a wide range of biological activities such as lowering blood pressure and heart rate, antimyocardial ischemia reperfusion injury, antiarrhythmia, and regulation of blood lipid levels. This review focuses on the chemical constituents and cardiovascular activities of Valeriana spp. PMID:26788113

  5. Chemical Components and Cardiovascular Activities of Valeriana spp.

    Directory of Open Access Journals (Sweden)

    Heng-Wen Chen

    2015-01-01

    Full Text Available Valeriana spp. is a flowering plant that is well known for its essential oils, iridoid compounds such as monoterpenes and sesquiterpenes, flavonoids, alkaloids, amino acids, and lignanoids. Valeriana spp. exhibits a wide range of biological activities such as lowering blood pressure and heart rate, antimyocardial ischemia reperfusion injury, antiarrhythmia, and regulation of blood lipid levels. This review focuses on the chemical constituents and cardiovascular activities of Valeriana spp.

  6. New, simple medium for selective, differential recovery of Klebsiella spp.

    OpenAIRE

    Tomás, J M; Ciurana, B; Jofre, J T

    1986-01-01

    A highly selective, differential medium for the enumeration and isolation of Klebsiella spp. was developed. With pure cultures, 100% recovery of Klebsiella spp. was observed. Recovery of Klebsiella spp. on MacConkey-inositol-potassium tellurite (MCIK) agar was as good as or better than on MacConkey-inositol-carbenicillin agar either with pure cultures or environmental samples. Recovery and percent colony confirmation with MCIK agar were greater and easier to obtain than for other proposed Kle...

  7. Screening of Gibberellic Acid Production by Pseudomonas SPP

    International Nuclear Information System (INIS)

    Khine Zar Wynn Myint; Khin Mya Lwin; Myo Myint

    2010-12-01

    The microbial gibberellic acid (GA3) production of Pseudomonas spp., was studied and qualitatively indentified by UV spectrophotometer. 20 strains of Pseudomonas spp., were isolated and screened the gibberellic acid productivily in King's B medium. Among them, only four strains can produce microbial gibberellic acid. The Rf values and colour appearance under UV were the same as authentic gibberellic acid. Moreover, the gibberellic acid producer strains were identified as Pseudomonas spp., by cultural, biochemical and drug sensitivity pattern.

  8. Avaliação da Brachiaria brizantha cv. marandu em sistemas silvipastoris Evaluation of Brachiaria brizantha cv. marandu in silvopastoral systems

    Directory of Open Access Journals (Sweden)

    G.R. Moreira

    2009-06-01

    Full Text Available Avaliou-se a degradabilidade in situ da matéria seca (MS da Brachiaria brizantha cv. marandu colhida em dois sistemas silvipastoris compostos pelas arbóreas, ipê felpudo (Zeyheria tuberculosa e aroeira (Myracrodruon urundeuva, localizados no bioma Cerrado, município de Lagoa Santa, MG. O período de coleta da forragem foi de dezembro de 2004 a abril de 2005. O delineamento utilizado foi em blocos ao acaso, segundo esquema de parcelas subdivididas, sendo as parcelas os tratamentos e as sub-parcelas os tempos de degradação. O sombreamento com as arbóreas não influenciou a degradabilidade in situ da matéria seca da forrageira.In situ degradability of dry matter of Brachiaria brizantha cv. marandu harvested in two silvopastoral systems was evaluated. One system was composed by Zeyheria tuberculosa tree and the other by Myracrodruon urundeuva, both located at Brazilian Savannah, Lagoa Santa, MG. A completely ramdomized block experimental design in a splitplot arrangement was used. The treatments were allocated in the plots and the times in the splitplot. No effect of shade was observed for in situ dry matter degradability.

  9. Effect of inoculation and nitrogen top-dressing on yields and fodder value of winter pea cv. Maksimirski ozimi in wheat cv. Sana mixture

    Directory of Open Access Journals (Sweden)

    Darko Uher

    2007-06-01

    Full Text Available Two year field trials (1999 - 2001 were performed to determine theinoculation seed winter pea and nitrogen top-dressing effect on number and active nodules of pea root and also on the green mass and dry matter yield of winter pea cv. Maksimirski ozimi and wheat cv. Sana mixture. Immediately before sowing the inoculation of pea seeds was accomplished by the indigenous variety of Rhizobium leguminosarum bv. viciae which belongs to the collection of Department of Microbiology at the Faculty of Agriculture University of Zagreb. The highest total nodule number on pea root (159 was determined on the inoculated variant as well as active nodule 144. Average mixture green mass yield were ranging from 24,65 t ha-1 (control up to 35,50 t ha-1 (inoculation. Total dry matter yields were ranging from 3,93 t ha-1 (control up to 5,66 t ha-1 (inoculation. Yields crude proteins pea in 2001 were ranging from 692 kg ha-1 (control up to 1058 kg ha-1 (inoculation and for wheat, those values ranged from 199 kg ha-1 (control up to 454 kg ha-1 (nitrogen top-dressing. Total crude proteins mixture yields were in range from 891 kg ha-1 (control up to 1360 kg ha-1 (inoculation.

  10. Effect of inoculation and nitrogen top-dressing on yields and fodder value of winter pea cv. Maksimirski ozimi in triticale cv. Clercal mixture

    Directory of Open Access Journals (Sweden)

    Darko Uher

    2006-07-01

    Full Text Available Two year field trials (1999-2001 were aimed to determine the inoculation seed winter pea and nitrogen top-dressing effect on number and active nodules of pea root and also on the green mass and dry matter yield of winter pea cv. Maksimirski ozimi and triticale cv. Clercal mixture. Immediately before sowing the inoculation of pea seeds was done by the indigenous variety of Rhizobium leguminosarum bv. viciae which belongs to the collection of Department of Microbiology at the Faculty of Agriculture University of Zagreb. The highest total nodule number on pea root (134 was determined on the inoculated variant as well as active nodule (120. Average mixture green mass yields were ranging from 20.9 t ha-1 (control up to 30.1 t ha-1 (inoculation + nitrogen top-dressing. Total dry matter yields were ranging from 3.71 t ha-1 (control up to 5.36 t ha-1 (nitrogen top-dressing. Pea crude protein yields in 2001 were ranging from 500 kg ha-1 (nitrogen top-dressing up to 642 kg ha-1 (inoculation and for triticale, those values were from 322 kg ha-1 (control up to 498 kg ha-1 (nitrogen top-dressing. Total mixture crude proteins yields were from 845 kg ha-1 (control up to 998 kg ha-1 (nitrogen top-dressing.

  11. Fox grape cv. Bordô (Vitis labrusca L. and grapevine cv. Chardonnay (Vitis vinifera L. cultivated in vitro under different carbohydrates, amino acids and 6-Benzylaminopurine levels

    Directory of Open Access Journals (Sweden)

    Dayse Cristina de Carvalho

    2013-04-01

    Full Text Available The aim of this work was to study the influence of sucrose and glucose, amino acids and BAP (6-Benzylaminopurine levels on in vitro shoot regeneration of fox grape cv. Bordô and grapevine cv. Chardonnay. The nodal segments from micropropagated material were used as explants and half-strength MS medium as the basal medium. Sucrose and glucose at 15, 30 and 45 g.L-1 were tested as a carbon source and the supplementation of adenine, asparagine, alanine, glycine, cysteine, glutamine, arginine was tested at 40 g.L-1. The BAP levels (1 and 5 μM in solid and double-phase media were evaluated and compared with a control medium without BAP. Bordô had best in vitro growth than Chardonnay. Sucrose was a better carbohydrate source than glucose for both the cultivars. Bordô and Chardonnay had different amino acid preferences for some parameters. In conclusion, for in vitro shoot regeneration from the nodal segments, culture on solid medium with 5 μM BAP, 15 g.L-1 sucrose for Bordô and 45 g.L-1 sucrose for Chardonnay showed better results. Similarly, the supplementation of 40 g.L-1 arginine for Bordô and 40 g.L-1 arginine or glycine for Chardonnay showed better results.

  12. Thermal stability of oils added with avocado (Persea americana cv. Hass) or olive (Olea europaea cv. Arbequina) leaf extracts during the French potatoes frying.

    Science.gov (United States)

    Jiménez, Paula; García, Paula; Bustamante, Andrés; Barriga, Andrés; Robert, Paz

    2017-04-15

    Effect of the addition of avocado (Persea americana cv. Hass) or olive (Olea europaea cv. Arbequina) hydroalcoholic leaf extracts (AHE and OHE, respectively) on thermal stability of canola oil (CO) and high oleic sunflower oil (HOSO) during French potatoes frying at 180°C was studied. The extracts were characterized by the total phenolic content, phenol chromatographic profiles and antioxidant activity. B-type trimer procyanidins were the major phenolic compounds identified in AHE. OHE showed higher phenol content, antioxidant activity regarding AHE. CO+OHE and HOSO+OHE decreased the formation of polar compounds and showed an anti-polymeric effect with respect to oils without extracts, whereas AHE extract showed a prooxidant effect on HOSO. Therefore, OHE showed an antioxidant effect on HOSO and CO under the studied conditions. In addition, all systems (CO+AHE, HOSO+AHE, CO+OHE and HOSO+OHE) increased the retention of tocopherols. These results demonstrate the potential utility of OHE as natural antioxidant for oils. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. Pathogenic and molecular characterisation of Pythium spp. inducing ...

    African Journals Online (AJOL)

    Pathogenic and molecular characterisation of Pythium spp. inducing root rot symptoms in other crops intercropped with beans in Southwestern Uganda. Virginia Gichuru, Robin Buruchara, Patrick Okori ...

  14. Efeito de reguladores de crescimento em uva apirênica, cv. BRS Clara Effect of growth regulators on the seedless grape cv. BRS Clara

    Directory of Open Access Journals (Sweden)

    Jair Costa Nachtigal

    2005-08-01

    Full Text Available O presente trabalho teve por objetivo verificar o efeito da aplicação de diferentes concentrações do ácido giberélico (AG3, do thidiazuron (TDZ e do forchlorfenuron (CPPU no aumento do tamanho dos cachos e das bagas e no teor de sólidos solúveis totais de uva sem semente, cv. BRS Clara. As plantas foram conduzidas no sistema de latada, sobre o porta-enxerto IAC 572, no espaçamento 2,5 x 2,0m e com irrigação por microaspersão. Os experimentos foram conduzidos na Estação Experimental de Viticultura Tropical, da Embrapa Uva e Vinho, em Jales-SP. Os reguladores de crescimento foram aplicados via pulverização localizada no cacho, utilizando-se de concentrações de 0 a 4mg.L-1 de CPPU; 0 a 10mg.L-1 de TDZ, e de 0 a 90mg.L-1 de AG3, isolados ou em conjunto. De modo geral, a aplicação dos reguladores de crescimento promove a melhoria da qualidade dos cachos da cv. BRS Clara; o uso do TDZ e do CPPU em conjunto com o AG3 produz um efeito sinérgico, proporcionando melhor resposta do que o uso isolado do AG3; os tratamentos com 60mg.L-1 de AG3; 20mg.L-1 de AG3 + 4mg.L-1 de CPPU, e 10mg.L-1 de AG3 + 5mg.L-1 de TDZ proporcionam os melhores resultados para o aumento do diâmetro das bagas; a aplicação de AG3 antes da floração da cv. BRS Clara provoca abortamento excessivo, reduzindo a qualidade comercial dos cachos; a utilização de concentrações elevadas dos reguladores reduz o teor de sólidos solúveis totais.The objective of this study was to evaluate the effect of different concentrations of gibberellic acid (AG3, thidiazuron (TDZ and forchlorfenuron (CPPU on the size of bunches and berries and on the total soluble solids content, when applied in different concentrations on the seedless grapes, cv. BRS Clara. The grapevines were grafted on the rootstock IAC 572, trained in the pergola system and, spaced 2.5 x 2.0m and irrigated by micro sprinklers. The experiments were carried out at the Embrapa Grape and Wine Experimental

  15. SPP retains interest in geothermal project

    International Nuclear Information System (INIS)

    Sobinkovic, B.

    2008-01-01

    Slovensky plynarensky priemysel (SPP) officially indicated that it intended to drop its project of using geothermal energy in the Kosicka kotlina. This spring it published an advert that it was looking for a company that wished to acquire a majority stake in the company, Geoterm Kosice. The company was established to commercially develop this geothermal source. But it seems SPP does not want to drop the project completely. It has kept some important cards, such as control over the land where the boreholes are located. Any company that wants to use geothermal energy needs a ruling issued by the Ministry of Environment defining the exploration area. Geothermal sources were found in the villages of Durkov, Svinica, Bidovce and Olsovany. Not so long ago the area was assigned to Geoterm but from May 9 2008 the area can be explored by Slovgeoterm. Both companies have the same majority shareholder - SPP. It controls 96% of Geoterm shares and 50% of Slovgeoterm. So far it has only officially announced its intention to sell the Geoterm shares. But as far as the use of the geothermal resource is concerned since May Slovgeoterm has played a key role.The company focuses on the utilization of geothermal energy. In addition to the project in the Kosice region, it has also participated in a project to heat more than a thousand flats using geothermal water in Galanta and a project to heat greenhouses in Podhajske. There are also other geothermal projects running in Presov and Michalovce. Icelandic company, Enex, with the same specialisation controls 28% of the company and a further 20% is owned by the investment group, NEFCO based in Helsinki. Two percent of the company is owned by its general director and the general proxy of Geoterm, Otto Halas. And so without the agreement of this company no-one can start any activities related to the utilization of geothermal energy. (authors)

  16. SPP retains interest in geothermal project

    International Nuclear Information System (INIS)

    Anon

    2007-01-01

    Slovensky plynarensky priemysel (SPP) officially indicated that it intended to drop its project of using geothermal energy in the Kosicka kotlina. This spring it published an advert that it was looking for a company that wished to acquire a majority stake in the company, Geoterm Kosice. The company was established to commercially develop this geothermal source. But it seems SPP does not want to drop the project completely. It has kept some important cards, such as control over the land where the boreholes are located Any company that wants to use geothermal energy needs a ruling issued by the Ministry of Environment defining the exploration area. Geothermal sources were found in the villages of Durkov, Svinica, Bidovce and Olsovany. Not so long ago the area was assigned to Geoterm but from May 9 the area can be explored by Slovgeoterm. Both companies have the same majority shareholder - SPP. It controls 96% of Geoterm shares and 50% of Slovgeoterm. So far it has only officially announced its intention to sell the Geoterm shares. But as far as the use of the geothermal resource is concerned since May Slovgeoterm has played a key role.The company focuses on the utilization of geothermal energy. In addition to the project in the Kosice region, it has also participated in a project to heat more than a thousand flats using geothermal water in Galanta and a project to heat greenhouses in Podhajske. There are also other geothermal projects running in Presov and Michalovce. Icelandic company, Enex, with the same specialisation controls 28% of the company and a further 20% is owned by the investment group, NEFCO based in Helsinki. Two percent of the company is owned by its general director and the general proxy of Geoterm, Otto Halas. And so without the agreement of this company no-one can start any activities related to the utilization of geothermal energy. (authors)

  17. GC-FID/MS Profiling of Supercritical CO2 Extracts of Peels from Citrus aurantium, C. sinensis cv. Washington navel, C. sinensis cv. Tarocco and C. sinensis cv. Doppio Sanguigno from Dubrovnik Area (Croatia).

    Science.gov (United States)

    Jerković, Igor; Drulžić, Jasmina; Marijanović, Zvonimir; Gugić, Mirko; Jokić, Stela; Roje, Marin

    2015-07-01

    The peels of Citrus aurantium L. and Citrus sinensis Osbeck cultivars from the Dubrovnik region (south Croatia) were extracted by supercritical CO2 at 40 degrees C and 10 MPa at 1.76 kg/h to obtain enriched extracts in comparison with simple pressing of the peels. The extracts were analyzed in detail by gas chromatography and mass spectrometry (GC-FID/MS). Relevant similarities among the peel oil compositions of C. aurantium and C. sinensis cultivars were found with limonene predominance (up to 54.3%). The principal oxygenated monoterpenes were linalool (3.0%-5.9%), α-terpineol (0.7%-2.4%), linalyl acetate (0.0%-5.0%), geranyl acetate (0.0%-0.4%), (Z)-citral (0.0%-1.8%) and (E)-citral (0.0%-1.9%). Several sesquiterpenes were found with minor percentages. Coumarin derivatives were identified in all the samples among the relevant compounds. Isogeijerin dominated in the peels of C. sinensis cv. Tarocco (15.3%) and C. aurantium (11.2%). Scoparone ranged from 0.1% to 0.5% in all the samples. Bergapten (up to 1.4%), osthole (up to 1.1%) and 7-methoxy-8-(2-formylpropyl)coumarin (up to 1.1%) were found mostly in C. sinensis cv. Doppio Sanguigno. It was possible to indicate a few other differences among the extracts such as higher percentage of linalool, linalyl and geranyl acetates, as well as the abundance of sabinene and isogeijerin in C. aurantium or the occurrence of β-sinensal in C. sinensis cultivars.

  18. Growth of Pseudomonas spp. in cottage cheese

    DEFF Research Database (Denmark)

    Østergaard, Nina Bjerre; Dalgaard, Paw

    of spoilage microorganisms in cottage cheese can cause undesirable alterations in flavour, odour, appearance and texture. Contamination and growth of psychrotolerant pseudomonads including Pseudomonas fragi and Pseudomonas putida has been reported for cottage cheese but the influence of these bacteria...... (pH 7.0) showed interesting results. Despite a lower pH value in the cottage cheese, compared to the dressing, more rapid growth was observed. This may be caused by insufficient amounts of oxygen in the cream dressing having a negative effect on growth of Pseudomonas spp. At 15˚C growth...

  19. First report of Colletotrichum spp. causing diseases on Capsicum spp. in Sabah, Borneo, Malaysia

    Directory of Open Access Journals (Sweden)

    H.K. Yun

    2009-08-01

    Full Text Available Blackish or orange liquid-like spots were found on (n=100 fruits of chillies (Capsicum sold in five local markets in Kota Kinabalu, Sabah, Malaysia. Colletotrichum gloeosporioides and C. capsici were identified as the causal agents of an anthracnose disease. This is the first report of Colletotrichum spp. as the causal agent of anthracnose infected chillies in Sabah.

  20. Control potential of Meloidogyne javanica and Ditylenchus spp. using fluorescent Pseudomonas and Bacillus spp.

    Directory of Open Access Journals (Sweden)

    Marcielly F. Turatto

    Full Text Available ABSTRACT Plant Growth Promoting Rhizobacteria (PGPR have different mechanisms of action in the development of plants, such as growth promotion, production of phytohormones and antibiotic substances and changes in root exudates. These help to control plant diseases. In order to evaluate the potential of microorganisms in the control of Meloidogyne javanica and Ditylenchus spp., five rhizobacteria isolated from rhizosphere of garlic cultivated in the Curitibanos (SC region were tested. Hatching chambers were set on Petri dishes, in which were added 10 mL of bacterial suspension and 1 mL of M. javanica eggs suspension, at the rate of 4500, on the filter paper of each chamber. The same procedure was performed with 300 juvenile Ditylenchus spp. The experimental design was completely randomized, with four replications. The evaluations were performed every 72 h for nine days. The antagonized population of nematodes was determined in Peters counting chamber, determining the percentage hatching (for M. javanica and motility (for Ditylenchus spp. Isolates CBSAL02 and CBSAL05 significantly reduced the hatching of M. javanica eggs (74% and 54.77%, respectively and the motility of Ditylenchus spp. (55.19% and 53.53%, respectively in vitro. Isolates were identified as belonging to the genera Pseudomonas (CBSAL05 and Bacillus (CBSAL02.

  1. Characterization of polyphenols and evaluation of antioxidant capacity in grape pomace of the cv. Malbec.

    Science.gov (United States)

    Antoniolli, Andrea; Fontana, Ariel R; Piccoli, Patricia; Bottini, Rubén

    2015-07-01

    Low molecular weight polyphenols (LMW-PPs) and anthocyanins, along with the antioxidant capacity, were assessed in grape pomace extract (GPE) of red grape (Vitis vinifera L.) cv. Malbec. Twenty-six phenolics (13 LMW-PPs and 13 anthocyanins) were characterized and quantified by HPLC-MWD and UPLC-ESI-MS. The maximum concentrations of LMW-PPs corresponded to the flavanols (+)-catechin and (-)-epicatechin, whereas malvidin-3-glucoside was the most abundant anthocyanin. Piceatannol, a stilbene analogue to resveratrol with higher antioxidant activity, was firstly identified and quantified in GPE of the cv. Malbec. The antioxidant activity for Malbec GPE determined by oxygen radical absorbance capacity (ORAC) assay was 2,756 μmol TEg(-1) GPE. Therefore, the data reported sustain the use of winemaking by-products as a cheap source of phenolic compounds suitable for biotechnological applications, as a strategy for sustainable oenology. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Potato cv. Romano reaction to primary and secondary infection with potato necrotic strain Y virus (PVYNTN

    Directory of Open Access Journals (Sweden)

    Drago Milošević

    2015-01-01

    Full Text Available Primary and secondary infections with PVYNTN were investigated on forty plants of the potato cv. Romano inoculated in a greenhouse in Serbia in 2012 and 2013. PVY isolates were collected from the potato growing region of Čačak and identified by ELISA and RT-PCR methods. The sequence of the Serbian isolate 3D (Acc. No. KJ946936 showed 100% match with seven PVY isolates deposited in GenBank and described as NTN. A significant difference was detected between PVYNTN symptoms exibited on leaves of the cv. Romano under primary and secondary infections. The findings are significant because they are based on symptoms observed, so that it is clear that there are two distinct types of infection: primary and secondary. Symptoms of primary and secondary infection were the same on potato tubers and had the form of necrotic rings.

  3. The control volume radial basis function method CV-RBF with Richardson extrapolation in geochemical problems

    Science.gov (United States)

    Florez, W. F.; Portapila, M.; Hill, A. F.; Power, H.; Orsini, P.; Bustamante, C. A.

    2015-03-01

    The aim of this paper is to present how to implement a control volume approach improved by Hermite radial basis functions (CV-RBF) for geochemical problems. A multi-step strategy based on Richardson extrapolation is proposed as an alternative to the conventional dual step sequential non-iterative approach (SNIA) for coupling the transport equations with the chemical model. Additionally, this paper illustrates how to use PHREEQC to add geochemical reaction capabilities to CV-RBF transport methods. Several problems with different degrees of complexity were solved including cases of cation exchange, dissolution, dissociation, equilibrium and kinetics at different rates for mineral species. The results show that the solution and strategies presented here are effective and in good agreement with other methods presented in the literature for the same cases.

  4. Sensitivity of Phaseolus vulgaris cv. `CIAP 7247F' plants to Glufosinate ammonium herbicide in greenhouse

    Directory of Open Access Journals (Sweden)

    Idalmis Bermúdez-Caraballoso

    2014-01-01

    Full Text Available Genetic breeding in Phaseolus by genetic transformation requires an efficient selection system. The present investigation was aimed to determine the minimum lethal concentration of glufosinate-ammonium (Finale ® in beans plants cv. `CIAP 7247F' grown in greenhouse. Different concentrations of the herbicide were applied to the foliage of plants in acclimatization phase (20, 30 y 40 mg l-1 and the control. Results showed that the minimum lethal concentration in plants in acclimatization phase was 30 mg l-1. Results also demonstrated that is possible the use of the herbicide as a selective agent of beans transformants cv. `CIAP 7247F' carrying the bar gene. Keywords: genetic transformation, herbicide, selective agent, tissue culture

  5. Vigor tests to evaluate the physiological quality of corn seeds cv. 'Sertanejo'

    Directory of Open Access Journals (Sweden)

    Daniela Vieira dos Anjos Sena

    Full Text Available ABSTRACT: The vigor tests are intended to monitor the quality of seeds as it allows reliably distinguish lots of low and high vigor, providing additional information to the germination test. So in this study the objective was to identify the most efficient vigor tests on stratification of lots of corn seeds cv. 'Sertanejo'. The experiment was conducted at Laboratory of Seed Analysis at the Center of agricultural Science of the Universidade Federal da Paraíba, using samples from 20 seed lots in a completely randomized design. For characterization of the lots it was evaluated water content, germination and vigor (cold test, electrical conductivity, accelerated aging, seedling emergence in field and germination at low temperatures. The accelerated aging, electrical conductivity and field emergence of seedlings tests are the most efficient for classification of lots of corn seeds cv. 'Sertanejo' in levels of vigor, been the seeds of lot 1 the most vigorous.

  6. Relationship between manganese toxicity and waterlogging tolerance in Zea mays L. cv. Saracura

    Directory of Open Access Journals (Sweden)

    Dayane Meireles da Silva

    2017-01-01

    Full Text Available The objective of this study was to evaluate the relationship between excess manganese and waterlogging tolerance in 18th selection cycle Zea mays L. cv. Saracura plants. Maize plants were transferred to plastic receptacles containing solutions with different concentrations of manganese. Leaves and roots were evaluated at the beginning of treatment and after 7, 14, and 21 days for chlorophyll content, biomass production and antioxidant metabolism. Mn was strongly translocated from the roots to the leaves, triggering a decrease in chlorophyll content. Excess Mn promoted an increase in reactive oxygen species that was accompanied by higher levels of antioxidative enzyme activity and lipid peroxidation. Zea mays L. cv. Saracura efficiently sequestered relatively large amounts of Mn in the leaves, with a significant impact on metabolism; however, we did not observe visual symptoms or a large decrease in biomass production.

  7. Xoconostle fruit (Opuntia matudae Scheinvar cv. Rosa) by-products as potential functional ingredients.

    Science.gov (United States)

    Morales, Patricia; Barros, Lillian; Ramírez-Moreno, Esther; Santos-Buelga, Celestino; Ferreira, Isabel C F R

    2015-10-15

    There is a lack of information on the potential use of xoconostle cultivars as sources of antioxidants for food, pharmaceutical and colorant industries. The aim of this study was to provide a phytochemical characterisation and antioxidant activity evaluation of Opuntia matudae Scheinvar cv. Rosa by-products (epicarp and endocarp mucilage's), in order to evaluate their interest as sources of functional ingredients for human or animal foods. These by-products showed a high content in glucose, citric and linoleic acids, tocopherols, and isorhamnetin-O-(di-deoxyhexosyl-hexoside) (mainly in epicarp), and presented relevant antioxidant properties. The obtained results support the use of O. matudae Scheinvar cv. Rosa agro-industrial by-products as functional food ingredients, namely for antioxidant-enriched formulations, instead of being discarded. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. Sim4CV: A Photo-Realistic Simulator for Computer Vision Applications

    KAUST Repository

    Müller, Matthias

    2018-03-24

    We present a photo-realistic training and evaluation simulator (Sim4CV) (http://www.sim4cv.org) with extensive applications across various fields of computer vision. Built on top of the Unreal Engine, the simulator integrates full featured physics based cars, unmanned aerial vehicles (UAVs), and animated human actors in diverse urban and suburban 3D environments. We demonstrate the versatility of the simulator with two case studies: autonomous UAV-based tracking of moving objects and autonomous driving using supervised learning. The simulator fully integrates both several state-of-the-art tracking algorithms with a benchmark evaluation tool and a deep neural network architecture for training vehicles to drive autonomously. It generates synthetic photo-realistic datasets with automatic ground truth annotations to easily extend existing real-world datasets and provides extensive synthetic data variety through its ability to reconfigure synthetic worlds on the fly using an automatic world generation tool.

  9. Slurry sampling in serum blood for mercury determination by CV-AFS

    International Nuclear Information System (INIS)

    Aranda, Pedro R.; Gil, Raul A.; Moyano, Susana; De Vito, Irma; Martinez, Luis D.

    2009-01-01

    The heavy metal mercury (Hg) is a neurotoxin known to have a serious health impact even at relatively low concentrations. A slurry method was developed for the sensitive and precise determination of mercury in human serum blood samples by cold vapor generation coupled to atomic fluorescence spectrometry (CV-AFS). All variables related to the slurry formation were studied. The optimal hydrochloric concentration and tin(II) chloride concentration for CV generation were evaluated. Calibration within the range 0.1-10 μg L -1 Hg was performed with the standard addition method, and compared with an external calibration. Additionally, the reliability of the results obtained was evaluated by analyzing mercury in the same samples, but submitted to microwave-assisted digestion method. The limit of detection was calculated as 25 ng L -1 and the relative standard deviation was 3.9% at levels around of 0.4 μg L -1 Hg

  10. An improved technique for quasi-static C-V measurements

    International Nuclear Information System (INIS)

    Turan, R.; Finstad, T.G.

    1990-10-01

    A new automated quasi-static C-V measurement technique for MOS capacitors has been developed. This techniques uses an integrating electrometer to measure the charge accumulated on a MOS capacitor in response of a small voltage step. Making use of the internal data storage system of a commercial electrometer and a personal computer, the charge Q on the MOS capacitor is measured as a function of time t and stored. The capacitance is then obtained by analyzing this Q-t data set. A Si MOS sample is measured and analyzed in terms of interface charges as an example. Advantages over a commercial quasi-static meter which uses similar measurement technique are presented. It is also shown that this technique is potentially capable of measuring both high and low frequency C-V curves simultaneously. 9 refs. 5 figs

  11. Electrochemical behaviour of gold modified with contaminated TMP amine adlayers studied by STM, CV, EPR

    Energy Technology Data Exchange (ETDEWEB)

    Krukowski, P. [Division of Physics and Technology of Nanometric Structures, Solid State Physics Department, University of Lodz, Pomorska 149/153, 90-236 Lodz (Poland)], E-mail: pkrukowski@std2.phys.uni.lodz.pl; Kowalczyk, P.J. [Division of Physics and Technology of Nanometric Structures, Solid State Physics Department, University of Lodz, Pomorska 149/153, 90-236 Lodz (Poland); Krzyczmonik, P. [Department of General and Inorganic Chemistry, University of Lodz, Narutowicza 68, 90-136 Lodz (Poland); Olejniczak, W.; Klusek, Z.; Puchalski, M. [Division of Physics and Technology of Nanometric Structures, Solid State Physics Department, University of Lodz, Pomorska 149/153, 90-236 Lodz (Poland); Gwozdzinski, K. [Department of Molecular Biophysics, University of Lodz, Banacha 12/16, 90-237 Lodz (Poland)

    2009-01-15

    Scanning tunnelling microscopy (STM), cyclic voltammetry (CV) and electron paramagnetic resonance spectroscopy (EPR) were used to investigate the influence of the TMP amine derivative on Au (1 1 1). The STM results show that the gold surface covered by the adlayer of the TMP derivative is easily modified (holes formation) after increasing the bias voltage to 0.5 V. The CV and EPR results show the electrochemical origin of observed STM topography changes. It is suggested that TMP could be oxidized to the nitroxyl TEMPO radical which adsorbs on Au in the form of an oxoammonium cation. Such an oxoammonium cation at the potential of 0.5 V forms a permanent complex of gold and the nitroxyl radical which could be easily desorbed during STM imaging.

  12. Cryopreservation of banana’s cv Grand Naine in vitro rhizomes

    Directory of Open Access Journals (Sweden)

    LUCIANA C.N. LONDE

    2017-10-01

    Full Text Available ABSTRACT The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryoprotectants and pre-freezing techniques are important factor. Therefore, the objective of this study was to verify the effects of different cryoprotectants with and without 1% phloroglucinol and pre-cooling periods on the development of a protocol for cryopreservation of in vitro rhizomes ofMusa accuminata(AAA cv Grand Naine banana. The addition of 1% phloroglucinol to the cryoprotective solutions, such as PVS2 enhanced recovery of cryopreserved banana rhizomes. In addition, pre-cooling of explants in ice for 3 hours in PVS2 + 1% of phloroglucinol allowed efficient cryopreservation of banana rhizomes, followed by successful recovery and regeneration of in vitro shoots of banana cv Grand Naine.

  13. ANALISIS WANPRESTASI KEAGENAN PENJUALAN KENDARAAN BERMOTOR (Studi CV Nobel Perdana Kabupaten Tulang Bawang

    Directory of Open Access Journals (Sweden)

    Heri Yulianto

    2017-03-01

    Full Text Available Standard by PT Sumber Trada Motor leads to no availability of vehicles to be sold by outlets CV Nobel Perdana. Giving great discounts and gifts, making consumers tend to buy directly from outlets PT Sumber Trada Motor. This agency system turns into competition due to the sales and market share CV Nobel Perdana excellent. The usefulness of this study as a researcher competence in the field of business law violations agreement. The problem of research is how the legal provisions breach agreement, the implementation of competition law to businesses which violate the agreement. Is expected to the Government through the KPPU to crack down on employers who violate the law No. 5 of 1999 on Competition, so as to control the businesses that destroy the independence of other businesses.   Keywords: Standard, Agreement, Agency

  14. Auditory extinction and dichotic listening cv task in cerebral infarction preliminary report

    Directory of Open Access Journals (Sweden)

    Mauro Muszkat

    1990-06-01

    Full Text Available Six stroke patients were studied using a dichotic listening¹ CV task, 4 with left hemisphere infarction, 2 with right hemisphere infarction. It was observed a «lesion--effect», a shift of hemisphere prevalence to the side opposite a brain lesion. The authors suggest that the lesion-effect can be explained by the auditory extinction phenomenon at the linguistic level.

  15. Xoconostle fruit (Opuntia matudae Scheinvar cv. Rosa) by-products as potential functional ingredients

    OpenAIRE

    Morales, Patricia; Barros, Lillian; Ramírez-Moreno, Esther; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.

    2015-01-01

    There is a lack of information on the potential use of xoconostle cultivars as sources of antioxidants for food, pharmaceutical and colorant industries. The aim of this study was to provide a phytochemical characterisation and antioxidant activity evaluation of Opuntia matudae Scheinvar cv. Rosa by-products (epicarp and endocarp mucilage's), in order to evaluate their interest as sources of functional ingredients for human or animal foods. These by-products showed a high content in glucose, c...

  16. Qualitative analysis of laser cutting of CV joints for automobile industry

    Science.gov (United States)

    Aboites, Vicente; Ramirez, Roberto; Rayas, Juan

    2005-02-01

    The optimization of an automatic laser cutting system is reported. This CO2 laser system assisted by an O2 gas jet is used in the cutting of Constant Velocity (CV) joint for the automotive industry. The experimental parameters varied in order to obtain cuts with low roughness were the laser power, cutting speed and oxygen pressure. A mathematical model is presented which explains many of the features of the qualitative optimization realized.

  17. Qualitative analysis of laser cutting of CV joints for the automobile industry

    Science.gov (United States)

    Aboites, V.; Ramírez, R.; Rayas, J.

    2006-02-01

    The optimization of an automatic laser cutting system is reported. This CO II laser system assisted by an O II gas jet is used in the cutting of Constant Velocity (CV) joint for the automotive industry. The experimental parameters varied in order to obtain cuts with low roughness were the laser power, cutting speed and oxygen pressure. A mathematical model is presented which explains many of the features of the qualitative optimization realized.

  18. Analisa Internal dan Eksternal pada CV. Gading Mas Surya Sidoarjo dalam Rangka Strategi Bersaing

    OpenAIRE

    Lesal, Indriani

    2015-01-01

    Strategi merupakan faktor internal yang penting untuk dipertimbangkan dalam perencanaan dan pengambilan keputusan. Setiap Perusahaan, baik yang bergerak di bidang jasa maupun non jasa, dalam melakukan kegiatan bisnis memerlukan strategi yang mampu menempatkan Perusahaan pada posisi yang terbaik, mampu bersaing serta terus berkembang dengan mengoptimalkan semua potensi sumber daya yang dimiliki. Tujuan penelitian ini adalah merumuskan strategi bersaing yang sesuai digunakan oleh CV. Gading Mas...

  19. Analisis Dan Rekayasa Supplier Relationship Management System Pada CV. Softcom Medan

    OpenAIRE

    Sukiman

    2012-01-01

    CV. SOFTCOM is a business entity engages in the computer sales and repair services. At the beginning of its establishment, the company did not see the importance of specialized brand in selling its product. The background of this study was the phenomenon in which many suppliers and different brands of the products found in the market that the company found it difficult to serve its customers claiming the damage of the sold products, the time of product delivered by the suppliers was frequentl...

  20. Phytoremediation of 4,4'-thiodiphenol (TDP) and other bisphenol derivatives by Portulaca oleracea cv.

    Science.gov (United States)

    Okuhata, Hiroshi; Ninagawa, Masahiko; Takemoto, Naomichi; Ji, Hezhe; Miyasaka, Hitoshi; Iwamoto, Ai; Nagae, Masaki; Ishibashi, Yasuhiro; Arizono, Koji

    2013-01-01

    4,4'-Thiodiphenol (TDP) is a bisphenol derivative, and there has been no report on TDP removal by any plants or pure bacterial cultures. The removal of TDP by Portulaca oleracea cv., a floricultural herbal plant, was examined with a hydroculture system, and 97% of TDP was removed after 4 days culture. Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  1. Exploring the SDSS Data Set with Linked Scatter Plots. I. EMP, CEMP, and CV Stars

    Energy Technology Data Exchange (ETDEWEB)

    Carbon, Duane F.; Henze, Christopher; Nelson, Bron C., E-mail: Duane.F.Carbon@nasa.gov [NASA Ames Research Center, NASA Advanced Supercomputing Facility, Moffett Field, CA, 94035-1000 (United States)

    2017-02-01

    We present the results of a search for extremely metal-poor (EMP), carbon-enhanced metal-poor (CEMP), and cataclysmic variable (CV) stars using a new exploration tool based on linked scatter plots (LSPs). Our approach is especially designed to work with very large spectrum data sets such as the SDSS, LAMOST, RAVE, and Gaia data sets, and it can be applied to stellar, galaxy, and quasar spectra. As a demonstration, we conduct our search using the SDSS DR10 data set. We first created a 3326-dimensional phase space containing nearly 2 billion measures of the strengths of over 1600 spectral features in 569,738 SDSS stars. These measures capture essentially all the stellar atomic and molecular species visible at the resolution of SDSS spectra. We show how LSPs can be used to quickly isolate and examine interesting portions of this phase space. To illustrate, we use LSPs coupled with cuts in selected portions of phase space to extract EMP stars, CEMP stars, and CV stars. We present identifications for 59 previously unrecognized candidate EMP stars and 11 previously unrecognized candidate CEMP stars. We also call attention to 2 candidate He ii emission CV stars found by the LSP approach that have not yet been discussed in the literature.

  2. Anthelmintic Activities of Aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana

    Directory of Open Access Journals (Sweden)

    Rong-Jyh Lin

    2014-02-01

    Full Text Available Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae, commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant, liriodenine (1, lysicamine (2, (--anonaine (3, (--asimilobine (4, (--caaverine (5, (--N-methylasimilobine (6, (--nuciferine (7, (--nornuciferine (8, (--roemerine (9, 7-hydroxydehydronuciferine (10 and cepharadione B (11 were isolated and identification and anthelmintic activities of aporphine was evaluated against Anisakis simplex and Hymenolepis nana. This study found that the above constituents killed H. nana or reduced their spontaneous movements (oscillation/peristalsis. However, the above constituents at various concentrations demonstrated no larvicidal effect or ability to halt spontaneous parasite movement for 72 h against A. simplex, respectively. In addition, according to an assay of cestocidal activity against H. nana and nematocidal activity against A. simplex, we found that the above compounds showed greater lethal efficacy on H. nana than against A. simplex. Further investigation showed that these above constituents have effects against peroxyl radicals under cestocidal effect. Together, these findings suggest that these constituents of Nelumbo nucifera Gaertn. cv. Rosa-plena might be used as anthelmintic agents against H. nana.

  3. Anti- Sporothrix spp. activity of medicinal plants

    Directory of Open Access Journals (Sweden)

    Stefanie Bressan Waller

    Full Text Available ABSTRACT Cases of sporotrichosis in humans and animals without satisfactory clinical response have increased, a warning sign of strains resistant to conventional antifungal agents. The urgent search for alternative therapies was an incentive for research on medicinal plants with anti-Sporothrix spp. properties. A bibliographic survey was performed based on scientific papers about in vitro and in vivo antifungal activity of essential oils and extracts of plants in differents solvents against the fungal of the Sporothrix schenckii complex. The study methodology consisted of a literature review in Google Scholar, Science Direct, Pubmed, Bireme and Springer link with papers from 1986 to 2015. We found 141 species of plants that were investigated, of which 100 species were concentrated in 39 botanical families that had confirmed anti-Sporothrix activity. Combretaceae, Asteraceae and Lamiaceae represented the botanical families with the greatest number of plants species with antifungal potential, using different methodologies. However, there are few studies with medicinal plants in experimental infection in animals that prove their activity in the treatment of sporotrichosis. It reinforces the need for further research related to standardization of in vitro methodologies and in vivo studies related to safety and to toxicity potential of these plants with anti-Sporothrix spp. activity.

  4. Strongyloides spp. infections of veterinary importance.

    Science.gov (United States)

    Thamsborg, Stig M; Ketzis, Jennifer; Horii, Yoichiro; Matthews, Jacqueline B

    2017-03-01

    This paper reviews the occurrence and impact of threadworms, Strongyloides spp., in companion animals and large livestock, the potential zoonotic implications and future research. Strongyloides spp. infect a range of domestic animal species worldwide and clinical disease is most often encountered in young animals. Dogs are infected with Strongyloides stercoralis while cats are infected with different species according to geographical location (Strongyloides felis, Strongyloides tumefaciens, Strongyloides planiceps and perhaps S. stercoralis). In contrast to the other species, lactogenic transmission is not a primary means of infection in dogs, and S. stercoralis is the only species considered zoonotic. Strongyloides papillosus in calves has been linked to heavy fatalities under conditions of high stocking density. Strongyloides westeri and Strongyloides ransomi of horses and pigs, respectively, cause only sporadic clinical disease. In conclusion, these infections are generally of low relative importance in livestock and equines, most likely due to extensive use of macrocyclic lactone anthelmintics and/or improved hygiene. Future prevalence studies need to include molecular typing of Strongyloides species in relation to different hosts. More research is urgently needed on the potential zoonotic capacity of Strongyloides from dogs and cats based on molecular typing, information on risk factors and mapping of transmission routes.

  5. Streptomyces spp. in the biocatalysis toolbox.

    Science.gov (United States)

    Spasic, Jelena; Mandic, Mina; Djokic, Lidija; Nikodinovic-Runic, Jasmina

    2018-04-01

    About 20,100 research publications dated 2000-2017 were recovered searching the PubMed and Web of Science databases for Streptomyces, which are the richest known source of bioactive molecules. However, these bacteria with versatile metabolism are powerful suppliers of biocatalytic tools (enzymes) for advanced biotechnological applications such as green chemical transformations and biopharmaceutical and biofuel production. The recent technological advances, especially in DNA sequencing coupled with computational tools for protein functional and structural prediction, and the improved access to microbial diversity enabled the easier access to enzymes and the ability to engineer them to suit a wider range of biotechnological processes. The major driver behind a dramatic increase in the utilization of biocatalysis is sustainable development and the shift toward bioeconomy that will, in accordance to the UN policy agenda "Bioeconomy to 2030," become a global effort in the near future. Streptomyces spp. already play a significant role among industrial microorganisms. The intention of this minireview is to highlight the presence of Streptomyces in the toolbox of biocatalysis and to give an overview of the most important advances in novel biocatalyst discovery and applications. Judging by the steady increase in a number of recent references (228 for the 2000-2017 period), it is clear that biocatalysts from Streptomyces spp. hold promises in terms of valuable properties and applicative industrial potential.

  6. CV Raman

    Indian Academy of Sciences (India)

    style, philosophy and motivations. We thus have here an authoritative biography of the most .... What motivated Raman to do the extraordinary things he did against all odds? What was the secret of his success? ... to Professor S. Chandrasekhar for permission to quote some of his statements. I am very indebted to Prof.

  7. Aminoethoxivinilglicina no controle do amadurecimento de frutos de caqui cv. Fuyu Aminoethoxyvinylglycine in the ripening control of persimmon fruits cv. Fuyu

    Directory of Open Access Journals (Sweden)

    Angela Fuentes Fagundes

    2006-04-01

    Full Text Available Objetivou-se avaliar o efeito de aminoethoxivinilglicina (AVG, aplicado na pós-colheita, no amadurecimento de frutos de caqui (Diospyros kaki L. cv. Fuyu, armazenados à temperatura de 0 ± 2 ºC. Os frutos foram imersos durante dois minutos em solução de AVG, na concentração de 0; 415; 830 e 1.200 g ha-1 e dissolvidos em água destilada e adição de espalhante adesivo (óleo vegetal a 0,02% (v/v, secos à temperatura ambiente no barracão e armazenados em câmara fria a 0 ± 2 ºC e 95±2 % UR, e avaliados aos 32 e 52 dias com relação à firmeza, sólidos solúveis, pH da polpa, acidez titulável e injúria por frio. O AVG mostrou-se promissor no uso pós-colheita em caqui, onde os frutos tratados conservaram índices de firmeza linear em função da dose e teores de AT, SS e pH aceitáveis para o 'Fuyu'. Porém os frutos apresentaram translucidez, característica de injúria por frio, indicando a necessidade de novos estudos para se compreender a fisiologia pós-colheita deste fruto.An experiment was carried out to analyze the effect of the application of Aminoethoxyvinylglycine (AVG in post harvest application in persimmon fruits (Diospyros kaki L. cv. Fuyu stored at a temperature of 0 ± 2ºC. The fruits were immersed for two minutes into an AVG solution at a concentration of 0; 415; 830; 1200 g ha-1 and dissolved in distillated water. Then it was added adhesive spreader (vegetable oil 0.02% (v/v, dried at room temperature and stored at 0 ± 2 ºC and 95±2 % humidity. The treatments were evaluated on the 32nd and 52nd days to check the firmness, soluble solids, pH of the pulp, tritable acidity and chilling injury. The AVG may be promising in post harvest use with persimmon fruits, where treated fruits preserved linear levels of firmness in function of the doses and TA, SS and pH levels are acceptable for cv. Fuyu. However, fruits showed translucency, which are characteristics of chilling injury, indicating that new studies are

  8. Genomic, proteomic and morphological characterization of two novel broad host lytic bacteriophages (phi)PD10.3 and (phi)PD23.1 infecting pectinolytic Pectobacterium spp. and Dickeya spp.

    NARCIS (Netherlands)

    Czajkowski, Robert; Ozymko, Zofia; de Jager, Victor; Siwinska, Joanna; Smolarska, Anna; Ossowicki, Adam; Narajczyk, Magdalena; Lojkowska, Ewa

    2015-01-01

    Pectinolytic Pectobacterium spp. and Dickeya spp. are necrotrophic bacterial pathogens of many important crops, including potato, worldwide. This study reports on the isolation and characterization of broad host lytic bacteriophages able to infect the dominant Pectobacterium spp. and Dickeya spp.

  9. Response of African eggplants to Fusarium spp. and identification of ...

    African Journals Online (AJOL)

    user

    2016-03-16

    Mar 16, 2016 ... Eggplant (Solanum spp.) production in Arumeru district and other parts of Africa is severely affected by wilting diseases of unknown etiology. Fusarium spp. characterized through morphological and sequence analysis of the translation elongation factor associated with Fusarium wilt of eggplants was.

  10. Severe Candida spp. infections: new insights into natural immunity.

    NARCIS (Netherlands)

    Meer, J.W.M. van der; Veerdonk, F.L. van de; Joosten, L.A.B.; Kullberg, B.J.; Netea, M.G.

    2010-01-01

    Invasive infections caused by Candida spp. are associated with high mortality. Colonisation by Candida spp. and the capacity of the host to recognise them as potential pathogens are essential steps in the development of these infections. The major pathogen-associated molecular patterns of Candida

  11. Genetic characterization of Salmonella and Shigella spp. isolates ...

    African Journals Online (AJOL)

    In order to detect and identify the target bacteria, samples were analysed by culture-dependent and culture-independent techniques (quantitative real-time PCR). Genetic relatedness was established using Sanger sequencing of the invA gene of Salmonella spp. and ipaH of Shigella spp. Results of this study displayed the ...

  12. Plant regulators for rooting of hardwood cuttings of blackberry plants cv. Xavante/ Reguladores vegetais no enraizamento de estacas lenhosas da amoreira-preta cv. Xavante

    Directory of Open Access Journals (Sweden)

    Renato Vasconcelos Botelho

    2008-08-01

    Full Text Available Aiming to evaluate the use of plant regulators for rooting of hardwood cuttings of blackberry cv. Xavante, two experiments were carried out in a greenhouse. In the first one the concentrations of indolil butyric acid varied in 0, 1000, 1500, 2000, 2500 and 3000 mg L-1. And in the second one, the plant regulator that was used was paclobutrazol at the concentrations of 0, 200, 400, 600, 800 and 1000 mg L-1. For each experiment the base of the cuttings were dipped in the solutions for ten seconds. After that, the cuttings were planted in rigid plastic vases with sand and kept under intermittent misty system. The following variables were evaluated, 71 days after planting: percentage of rooted cuttings, percentage of callus presence, average length of roots, number of roots, fresh weight of roots, percentage of sprouting. The treatment of hardwood cuttings with 2000 mg L-1 IBA had the greatest percentage of rooting (60%. Nevertheless, the treatment with 1000 mg L-1 IBA attained already high rooting percentage (56%, with 96% of sprouted cuttings and 17.6 roots per cutting. Cuttings treated with PBZ presented greater callus presence but they did not root, thus it is not possible to recommend this product for rooting of blackberry plants cv. Xavante among the tested concentrations.Com o objetivo de avaliar o emprego de reguladores vegetais no enraizamento de estacas lenhosas da amora-preta cv. Xavante dois experimentos foram conduzidos em casa-de-vegetação. No primeiro, empregou-se o ácido indol butírico (AIB nas concentrações de 0, 1000, 1500, 2000, 2500, 3000 mg L-1. No segundo, o regulador utilizado foi o paclobutrazol (PBZ nas concentrações de 0, 200, 400, 600, 800, 1000 mg L-1. Em ambos os experimentos as estacas ficaram imersas por 10 segundos nas respectivas soluções. Posteriormente foram plantados em vasos de plástico rígido contendo areia, sendo mantidas em casade- vegetação sob nebulização intermitente. Após 71 dias do plantio

  13. BACTERIAL CONTAMINATION CONTROL IN BANANA EXPLANTS (Musa AAA cv. CAIPIRA) CONTROLE DE BACTÉRIAS CONTAMINANTES EM EXPLANTES DE BANANEIRA (Musa AAA cv. CAIPIRA)

    OpenAIRE

    Juliana Domingues Lima; Wilson da Silva Moraes

    2007-01-01

    Esse trabalho teve por objetivo testar métodos de controle de contaminação bacteriana no processo de multiplicação in vitro de bananeira (Musa AAA cv. Caipira), utilizando-se hipoclorito de sódio (NaOCl), antibiótico rifampicina e suas combinações. Não houve oxidação excessiva dos explantes após a imersão em NaOCl ou rifampicina. O melhor tratamento para explantes recém isolados foi imersão em NaOCl a 1% (v/v), dura...

  14. WNT Inhibitory Activity of Malus Pumila miller cv Annurca and Malus domestica cv Limoncella Apple Extracts on Human Colon-Rectal Cells Carrying Familial Adenomatous Polyposis Mutations

    Directory of Open Access Journals (Sweden)

    Gennaro Riccio

    2017-11-01

    Full Text Available Inhibitors of the Wingless-related Integration site (WNT/β-catenin pathway have recently been under consideration as potential chemopreventive agents against Familial Adenomatous Polyposis (FAP. This autosomal-dominant syndrome is caused by germline mutations in the gene coding for the protein APC and leads to hyperactivation of the WNT/β-catenin signaling pathway, uncontrolled intestinal cell proliferation and formation of adenocarcinomas. The aim of the present work was to: (i test, on in vitro cultures of cells carrying FAP mutations and on ex vivo biopsies of FAP patients, the WNT inhibitory activity of extracts from two common southern Italian apples, Malus pumila Miller cv. ‘Annurca’ and Malus domestica cv ‘Limoncella’; (ii identify the mechanisms underpinning their activities and; (iii evaluate their potency upon gastrointestinal digestion. We here show that both Annurca and Limoncella apple extracts act as WNT inhibitors, mostly thanks to their polyphenolic contents. They inhibit the pathway in colon cells carrying FAP mutations with active dilutions falling in ranges close to consumer-relevant concentrations. Food-grade manufacturing of apple extracts increases their WNT inhibitory activity as result of the conversion of quercetin glycosides into the aglycone quercetin, a potent WNT inhibitor absent in the fresh fruit extract. However, in vitro simulated gastrointestinal digestion severely affected WNT inhibitory activity of apple extracts, as result of a loss of polyphenols. In conclusion, our results show that apple extracts inhibit the WNT pathway in colon cells carrying FAP mutations and represent a potential nutraceutical alternative for the treatment of this pathology. Enteric coating is advisable to preserve the activity of the extracts in the colon-rectal section of the digestive tract.

  15. WNT Inhibitory Activity of Malus Pumila miller cv Annurca and Malus domestica cv Limoncella Apple Extracts on Human Colon-Rectal Cells Carrying Familial Adenomatous Polyposis Mutations.

    Science.gov (United States)

    Riccio, Gennaro; Maisto, Maria; Bottone, Sara; Badolati, Nadia; Rossi, Giovanni Battista; Tenore, Gian Carlo; Stornaiuolo, Mariano; Novellino, Ettore

    2017-11-18

    Inhibitors of the Wingless-related Integration site (WNT)/β-catenin pathway have recently been under consideration as potential chemopreventive agents against Familial Adenomatous Polyposis (FAP). This autosomal-dominant syndrome is caused by germline mutations in the gene coding for the protein APC and leads to hyperactivation of the WNT/β-catenin signaling pathway, uncontrolled intestinal cell proliferation and formation of adenocarcinomas. The aim of the present work was to: (i) test, on in vitro cultures of cells carrying FAP mutations and on ex vivo biopsies of FAP patients, the WNT inhibitory activity of extracts from two common southern Italian apples, Malus pumila Miller cv. 'Annurca' and Malus domestica cv 'Limoncella'; (ii) identify the mechanisms underpinning their activities and; (iii) evaluate their potency upon gastrointestinal digestion. We here show that both Annurca and Limoncella apple extracts act as WNT inhibitors, mostly thanks to their polyphenolic contents. They inhibit the pathway in colon cells carrying FAP mutations with active dilutions falling in ranges close to consumer-relevant concentrations. Food-grade manufacturing of apple extracts increases their WNT inhibitory activity as result of the conversion of quercetin glycosides into the aglycone quercetin, a potent WNT inhibitor absent in the fresh fruit extract. However, in vitro simulated gastrointestinal digestion severely affected WNT inhibitory activity of apple extracts, as result of a loss of polyphenols. In conclusion, our results show that apple extracts inhibit the WNT pathway in colon cells carrying FAP mutations and represent a potential nutraceutical alternative for the treatment of this pathology. Enteric coating is advisable to preserve the activity of the extracts in the colon-rectal section of the digestive tract.

  16. A combined SEM, CV and EIS study of multi-layered porous ceramic reactors for flue gas purification

    DEFF Research Database (Denmark)

    He, Zeming; Andersen, Kjeld Bøhm; Nygaard, Frederik Berg

    2013-01-01

    The effect of sintering temperature of 12-layered porous ceramic reactors (comprising 5 cells) was studied using scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The difference in microstructures of the reactors was evaluated by SEM...

  17. Automated and connected vehicle (AV/CV) test bed to improve transit, bicycle, and pedestrian safety : technical report.

    Science.gov (United States)

    2017-02-01

    Crashes involving transit vehicles, bicyclists, and pedestrians are a concern in Texas, especially in urban areas. This research explored the potential of automated and connected vehicle (AV/CV) technology to reduce or eliminate these crashes. The pr...

  18. Application of the Geriatric Anxiety Inventory-Chinese Version (GAI-CV) to older people in Beijing communities.

    Science.gov (United States)

    Yan, Yue; Xin, Tao; Wang, Dahua; Tang, Dan

    2014-03-01

    The Geriatric Anxiety Inventory (GAI) was developed to assess anxiety in older adults. The objectives of this work were as follows: (a) to analyze the psychometric properties of the Chinese version of the GAI (GAI-CV), and (b) to explore the extent of anxiety and related factors in the elderly Chinese residents of Beijing. Participants in this study included 1,047 people (59.4% female) more than 60 years old who were living in the community. They were randomly selected from 15 communities in Beijing. Basic information was collected. Anxiety was measured using the GAI-CV, the Self-Rating Anxiety Scale (SAS), and the Beck Anxiety Inventory (BAI). The GAI-CV exhibited good internal consistency (Cronbach's α = 0.94) and demonstrated good concurrent validity against the SAS (r = 0.52, p = 0.018) and the BAI (r = 0.560, p = 0.000). Item response theory (IRT) analyses showed that the items of the GAI-CV exhibited high difficulty (0.97-2) and discrimination parameters (1.91-5.33). The items exhibited information parameters greater than 1.25 with the exceptions of items 2, 12, and 18. The GAI-CV scores were significantly associated with gender, age, and chronic disease. However, no significant differences due to marriage or education were found. The GAI is a new scale that was specifically designed to measure anxiety in older people. The results of this study suggest that the GAI-CV had good psychometric properties, but some items need to be modified. IRT analyses indicated that the GAI-CV provided good measures of anxiety across the moderately high to very high levels. The GAI-CV may be a useful instrument for further research studies aimed at analyzing high-level anxiety among older adults in China.

  19. Physiological and morphological responces of Brachiaria spp. to flooding Respostas morfológicas e fisiológicas de Brachiaria spp. ao alagamento do solo

    Directory of Open Access Journals (Sweden)

    MOACYR BERNARDINO DIAS-FILHO

    2000-10-01

    Full Text Available The physiological and morphological responses of the forage grasses Brachiaria brizantha cv. Marandu, B. decumbens and B. humidicola were compared for plants grown in pots under flooding and well-drained conditions for 14 days. Flooding reduced specific leaf area and biomass allocation to roots in all species and enhanced leaf senescence in B. brizantha and B. decumbens. Relative growth rate was reduced by flooding in B. brizantha and B. decumbens, but not in B. humidicola.Leaf elongation rate was unaffected by flooding in B. decumbens and B. humidicola, but declined in B. brizantha since the first day of flooding. Net photosynthesis and leaf chlorophyll content were reduced by flooding in B. brizantha; however, no flooding effect could be detected in the other two species. For all species, there was a close relationship between net photosynthesis and stomatal conductance under flooding. These results show that the studied species have distinct degrees of tolerance to flood, B. brizantha is intolerant, B. decumbens is moderately tolerant and B. humidicola is tolerant. Because leaf elongation rate was immediately depressed by flooding only in B. brizantha, this measurement could be appropriate as an early detection mechanism for relative flood tolerance in Brachiaria spp.As respostas morfológicas e fisiológicas de Brachiaria brizantha cv. Marandu, B. decumbens e B. humidicola foram comparadas em plantas cultivadas em vasos, sob solo alagado e bem drenado durante 14 dias. O alagamento reduziu a área foliar específica e a alocação de biomassa para as raízes em todas as três espécies e aumentou a senescência foliar em B. brizantha e B. decumbens. O alagamento reduziu a taxa de crescimento relativo em B. brizantha e B. decumbens, mas não em B. humidicola. A taxa de elongação foliar não foi afetada pelo alagamento em B. decumbens e B. humidicola, mas diminuiu em B. brizantha desde o primeiro dia de alagamento. A fotossíntese líquida e

  20. Pseudomonas spp. convert metmyoglobin into deoxymyoglobin.

    Science.gov (United States)

    Motoyama, Michiyo; Kobayashi, Miho; Sasaki, Keisuke; Nomura, Masaru; Mitsumoto, Mitsuru

    2010-01-01

    Meat 'reddening' by bacteria was observed in chilled beef. To identify the reddening bacteria, isolates were inoculated onto beef and the changes in CIE L*a*b* values monitored. As a result, two Pseudomonas spp., including Pseudomonas fragi which is commonly observed in raw meat, were selected and identified as reddening bacteria. The reddening was coincidentally occurred with the appearance of slime, and the increase in thiobarbituric acid-reactive substances (TBARS) was simultaneously suppressed. In myoglobin-containing nutrient broth, it is shown spectroscopically that P. fragi converted metmyoglobin into deoxymyoglobin. It was concluded that the meat reddening was due to the formation of deoxymyoglobin, induced by the very-low-oxygen tension brought about by Pseudomonad's oxygen consumption: This oxygen depletion simultaneously suppressed TBARS increase.

  1. Rhabdochlamydia spp. in an Oregon raptor.

    Science.gov (United States)

    Jouffroy, Sophie J; Schlueter, Andrew H; Bildfell, Robert J; Rockey, Daniel D

    2016-07-01

    PCR-based approach was used to examine the rate of Chlamydia positivity in raptors from wild bird rehabilitation centers in Oregon. Three of 82 birds were identified as positive for Chlamydia with this PCR. Sequence analysis of 16S ribosomal DNA from 2 of these birds confirmed the presence of DNA from phylum Chlamydiae. One bird was positive for Chlamydia psittaci in both choanal and cloacal swabs. The second bird, a louse-infested red-tailed hawk, had evidence of choanal colonization by "Candidatus Rhabdochlamydia" spp. Our study describes evidence of this Chlamydia-like organism in the United States. This survey also suggests that the carriage rate of C. psittaci is low in raptors in Oregon wild bird rehabilitation centers, and that care must be taken in the design of PCR primers for phylum Chlamydiae such that colonization by insect endosymbionts is not mistaken for an infection by known chlamydial pathogens. © 2016 The Author(s).

  2. Antifungal Streptomyces spp. associated with the infructescences of Protea spp. in South Africa

    Directory of Open Access Journals (Sweden)

    Zander Human

    2016-11-01

    Full Text Available Common saprophytic fungi are seldom present in Protea infructescences, which is strange given the abundance of mainly dead plant tissue in this moist protected environment. We hypothesized that the absence of common saprophytic fungi in Protea infructescences could be due to a special symbiosis where the presence of microbes producing antifungal compounds protect the infructescence. Using a culture based survey, employing selective media and in vitro antifungal assays, we isolated antibiotic producing actinomycetes from infructescences of Protea repens and P. neriifolia from two geographically separated areas. Isolates were grouped into three different morphological groups and appeared to be common in the Protea spp. examined in this study. The three groups were supported in 16S rRNA and multi-locus gene trees and were identified as potentially novel Streptomyces spp. All of the groups had antifungal activity in vitro. Streptomyces sp. Group 1 had inhibitory activity against all tested fungi and the active compound produced by this species was identified as fungichromin. Streptomyces spp. Groups 2 and 3 had lower inhibition against all tested fungi, while Group 3 showed limited inhibition against Candida albicans and Sporothrix isolates. The active compound for Group 2 was also identified as fungichromin even though its production level was much lower than Group 1. The antifungal activity of Group 3 was linked to actiphenol. The observed antifungal activity of the isolated actinomycetes could contribute to protection of the plant material against common saprophytic fungi, as fungichromin was also detected in extracts of the infructescence. The results of this study suggest that the antifungal Streptomyces spp. could play an important role in defining the microbial population associated with Protea infructescences.

  3. Susceptibilidad de genotipos de Solanum spp. al nematodo causante del nudo radical Meloidogyne spp. (chitwood

    Directory of Open Access Journals (Sweden)

    Cristian Gelpud Chaves

    2011-01-01

    Full Text Available El cultivo del lulo (Solanum quitoense L. presenta una disminución en su productividad, debido al ataque de patógenos como el nematodo del nudo radical Meloidogyne spp., en el Departamento de Nariño (Colombia, se han reportado incidencias cercanas al 79%, y pérdidas del 50%. En la presente investigación, se colectaron 45 genotipos de (Solanum quitoense L. en los Departamentos de Nariño y Putumayo y 4 genotipos silvestres (S. mammosum, S. hirtum, S. marginatum y S. umbellatum buscando fuentes de resistencia al nematodo. Se inocularon 9 plantas de cada genotipo de dos meses de edad con 10000 huevos de Meloidogyne spp., dejando tres testigos por cada material. Las variables evaluadas fueron: altura de planta, severidad, incidencia, peso fresco (tallo y raíz y especies prevalentes de Meloidogyne spp. Se hizo una clasificación de genotipos mediante escala de resistencia y regresión entre la severidad y las demás variables para establecer el efecto de Meloidogyne spp. sobre los genotipos de planta. Los resultados mostraron 100% de incidencia del nematodo en todos los genotipos, 2.04% genotipos resistentes, 34.7% moderadamente resistentes, 42.8% moderadamente susceptibles, 18.3% susceptibles, y 2.04% altamente susceptibles. El genotipo SQbr05 resistente, no se vio afectado por la severidad, al contrario SQbc04 genotipo susceptible, mostró reducciones significativas en peso fresco de tallo y raíz, (R² = 0.71 y 0.98, el genotipo silvestre (S. mammosum es altamente susceptible, Meloidogyne incognita presentó 55.31% de presencia. El genotipo SQbr05 es promisorio para ser evaluado en campo.

  4. Antifungal Streptomyces spp. Associated with the Infructescences of Protea spp. in South Africa

    Science.gov (United States)

    Human, Zander R.; Moon, Kyuho; Bae, Munhyung; de Beer, Z. Wilhelm; Cha, Sangwon; Wingfield, Michael J.; Slippers, Bernard; Oh, Dong-Chan; Venter, Stephanus N.

    2016-01-01

    Common saprophytic fungi are seldom present in Protea infructescences, which is strange given the abundance of mainly dead plant tissue in this moist protected environment. We hypothesized that the absence of common saprophytic fungi in Protea infructescences could be due to a special symbiosis where the presence of microbes producing antifungal compounds protect the infructescence. Using a culture based survey, employing selective media and in vitro antifungal assays, we isolated antibiotic producing actinomycetes from infructescences of Protea repens and P. neriifolia from two geographically separated areas. Isolates were grouped into three different morphological groups and appeared to be common in the Protea spp. examined in this study. The three groups were supported in 16S rRNA and multi-locus gene trees and were identified as potentially novel Streptomyces spp. All of the groups had antifungal activity in vitro. Streptomyces sp. Group 1 had inhibitory activity against all tested fungi and the active compound produced by this species was identified as fungichromin. Streptomyces spp. Groups 2 and 3 had lower inhibition against all tested fungi, while Group 3 showed limited inhibition against Candida albicans and Sporothrix isolates. The active compound for Group 2 was also identified as fungichromin even though its production level was much lower than Group 1. The antifungal activity of Group 3 was linked to actiphenol. The observed antifungal activity of the isolated actinomycetes could contribute to protection of the plant material against common saprophytic fungi, as fungichromin was also detected in extracts of the infructescence. The results of this study suggest that the antifungal Streptomyces spp. could play an important role in defining the microbial population associated with Protea infructescences. PMID:27853450

  5. Detection of relapsing fever Borrelia spp., Bartonella spp. and Anaplasmataceae bacteria in argasid ticks in Algeria.

    Directory of Open Access Journals (Sweden)

    Ismail Lafri

    2017-11-01

    Full Text Available Argasid ticks (soft ticks are blood-feeding arthropods that can parasitize rodents, birds, humans, livestock and companion animals. Ticks of the Ornithodoros genus are known to be vectors of relapsing fever borreliosis in humans. In Algeria, little is known about relapsing fever borreliosis and other bacterial pathogens transmitted by argasid ticks.Between May 2013 and October 2015, we investigated the presence of soft ticks in 20 rodent burrows, 10 yellow-legged gull (Larus michahellis nests and animal shelters in six locations in two different bioclimatic zones in Algeria. Six species of argasid ticks were identified morphologically and through 16S rRNA gene sequencing. The presence and prevalence of Borrelia spp., Bartonella spp., Rickettsia spp. and Anaplasmataceae was assessed by qPCR template assays in each specimen. All qPCR-positive samples were confirmed by standard PCR, followed by sequencing the amplified fragments. Two Borrelia species were identified: Borrelia hispanica in Ornithodoros occidentalis in Mostaganem, and Borrelia cf. turicatae in Carios capensis in Algiers. One new Bartonella genotype and one new Anaplasmataceae genotype were also identified in Argas persicus.The present study highlights the presence of relapsing fever borreliosis agents, although this disease is rarely diagnosed in Algeria. Other bacteria of unknown pathogenicity detected in argasid ticks which may bite humans deserve further investigation.

  6. Detection of relapsing fever Borrelia spp., Bartonella spp. and Anaplasmataceae bacteria in argasid ticks in Algeria.

    Science.gov (United States)

    Lafri, Ismail; El Hamzaoui, Basma; Bitam, Idir; Leulmi, Hamza; Lalout, Reda; Mediannikov, Oleg; Chergui, Mohamed; Karakellah, Mohamed; Raoult, Didier; Parola, Philippe

    2017-11-01

    Argasid ticks (soft ticks) are blood-feeding arthropods that can parasitize rodents, birds, humans, livestock and companion animals. Ticks of the Ornithodoros genus are known to be vectors of relapsing fever borreliosis in humans. In Algeria, little is known about relapsing fever borreliosis and other bacterial pathogens transmitted by argasid ticks. Between May 2013 and October 2015, we investigated the presence of soft ticks in 20 rodent burrows, 10 yellow-legged gull (Larus michahellis) nests and animal shelters in six locations in two different bioclimatic zones in Algeria. Six species of argasid ticks were identified morphologically and through 16S rRNA gene sequencing. The presence and prevalence of Borrelia spp., Bartonella spp., Rickettsia spp. and Anaplasmataceae was assessed by qPCR template assays in each specimen. All qPCR-positive samples were confirmed by standard PCR, followed by sequencing the amplified fragments. Two Borrelia species were identified: Borrelia hispanica in Ornithodoros occidentalis in Mostaganem, and Borrelia cf. turicatae in Carios capensis in Algiers. One new Bartonella genotype and one new Anaplasmataceae genotype were also identified in Argas persicus. The present study highlights the presence of relapsing fever borreliosis agents, although this disease is rarely diagnosed in Algeria. Other bacteria of unknown pathogenicity detected in argasid ticks which may bite humans deserve further investigation.

  7. Pertumbuhan Optimum Penicillium spp. dan Cunninghamella spp. yang Diisolasi dari Pakan dan Efek Toksiknya pada Mencit (Mus musculus

    Directory of Open Access Journals (Sweden)

    N. Ramli

    2009-04-01

    Full Text Available Fungi are a major microorganism present in the feedstuffs that able to reduce nutritive value and produce toxin that harmful for animal. Penicillium spp. and Cunninghamella spp. were dominant present in the feedstuffs in tropical regions. The objectives of this study were to find out the optimum pH and temperature of Penicillium spp. and Cunninghamella spp. on agar media and to find out the effect of feeding diet containing corn contaminated with the fungi at level of 0%, 50% and 100% on the performance and relative organ weight of 45 mice (Mus musculus. Cunninghamella spp. grew at pH range of 3-9, and Penicillium spp. at pH range of 3-7. The temperature for the optimum growth of both fungi was on room temperature (28±2 oC. Feed consumption and daily gain (ADG of mice were not significantly affected by content of corn contaminated with Cunninghamella spp. Conversely, corn contaminated with Penicillium spp. significantly (P<0.05 reduced feed consumption and ADG of mice. Treatments had no effect on liver and hearth relative weight, but significantly influenced relative weight of kidney and lymph. Lymph relative weight of mice fed ration containing Penicillium-contaminated corn was lower (P<0.05 than that of control. Mice treated with contaminated corn from both fungi at the level 100% also significantly (P<0.05 had higher kidney relative weight than that of control. It was concluded that the toxic effect of Penicillium spp. was higher than that of Cunninghamella spp.

  8. Leptospira spp. in Rodents and Shrews in Germany

    Directory of Open Access Journals (Sweden)

    Anne Mayer-Scholl

    2014-07-01

    Full Text Available Leptospirosis is an acute, febrile disease occurring in humans and animals worldwide. Leptospira spp. are usually transmitted through direct or indirect contact with the urine of infected reservoir animals. Among wildlife species, rodents act as the most important reservoir for both human and animal infection. To gain a better understanding of the occurrence and distribution of pathogenic leptospires in rodent and shrew populations in Germany, kidney specimens of 2973 animals from 11 of the 16 federal states were examined by PCR. Rodent species captured included five murine species (family Muridae, six vole species (family Cricetidae and six shrew species (family Soricidae. The most abundantly trapped animals were representatives of the rodent species Apodemus flavicollis, Clethrionomys glareolus and Microtus agrestis. Leptospiral DNA was amplified in 10% of all animals originating from eight of the 11 federal states. The highest carrier rate was found in Microtus spp. (13%, followed by Apodemus spp. (11% and Clethrionomys spp. (6%. The most common Leptospira genomospecies determined by duplex PCR was L. kirschneri, followed by L. interrogans and L. borgpetersenii; all identified by single locus sequence typing (SLST. Representatives of the shrew species were also carriers of Leptospira spp. In 20% of Crocidura spp. and 6% of the Sorex spp. leptospiral DNA was detected. Here, only the pathogenic genomospecies L. kirschneri was identified.

  9. Leptospira spp. in Rodents and Shrews in Germany

    Science.gov (United States)

    Mayer-Scholl, Anne; Hammerl, Jens Andre; Schmidt, Sabrina; Ulrich, Rainer G.; Pfeffer, Martin; Woll, Dietlinde; Scholz, Holger C.; Thomas, Astrid; Nöckler, Karsten

    2014-01-01

    Leptospirosis is an acute, febrile disease occurring in humans and animals worldwide. Leptospira spp. are usually transmitted through direct or indirect contact with the urine of infected reservoir animals. Among wildlife species, rodents act as the most important reservoir for both human and animal infection. To gain a better understanding of the occurrence and distribution of pathogenic leptospires in rodent and shrew populations in Germany, kidney specimens of 2973 animals from 11 of the 16 federal states were examined by PCR. Rodent species captured included five murine species (family Muridae), six vole species (family Cricetidae) and six shrew species (family Soricidae). The most abundantly trapped animals were representatives of the rodent species Apodemus flavicollis, Clethrionomys glareolus and Microtus agrestis. Leptospiral DNA was amplified in 10% of all animals originating from eight of the 11 federal states. The highest carrier rate was found in Microtus spp. (13%), followed by Apodemus spp. (11%) and Clethrionomys spp. (6%). The most common Leptospira genomospecies determined by duplex PCR was L. kirschneri, followed by L. interrogans and L. borgpetersenii; all identified by single locus sequence typing (SLST). Representatives of the shrew species were also carriers of Leptospira spp. In 20% of Crocidura spp. and 6% of the Sorex spp. leptospiral DNA was detected. Here, only the pathogenic genomospecies L. kirschneri was identified. PMID:25062275

  10. Importance of winter pea cv. Maksimirski rani in milk production on family farms

    Directory of Open Access Journals (Sweden)

    Darko Uher

    2010-03-01

    Full Text Available Forage pea (Pisum sativum L. is gaining importance as a forage legume in the Republic of Croatia. Pea seed contains 20-30 percent of protein, it is utilized without thermal treatment in feeding different types and categories of livestock, and with stable yield it provides an appreciable income per hectare. Two-year field trials (2005-2006 were carried out to determine the effect of winter pea seed inoculation and nitrogen top-dressing on the number and mass (g/plant-1 of root nodules and also on the yield and quality of winter pea cv. Maksimirski rani in a mixture with wheat cv. Sana. Just before sowing, pea seeds were inoculated with the strain Rhizobium leguminosarum bv. viciae 1001 from the microbial collection of the Department of Microbiology, Faculty of Agriculture, University of Zagreb. The highest number of root nodules (43 nodules/plant, as well as the highest nodule mass (0.219 g/plant-1 were determined in the inoculated variant. The highest number of pods (19.0 and seeds per plant (60 were determined in the inoculated variant as well. The highest 1000-seed mass (132 g and seed mass per plant (7.93 g were also determined in the inoculated variant. Average pea seed yield ranged from 2949 kg ha-1 (control up to 3353 kg ha-1 (inoculation. The conclusion of this research is that the highest seed (3353 kg ha-1 and crude protein yields (833 kg ha-1 were obtained with inoculated forage winter pea cv. Maksimirski rani. Seed inoculation of the studied pea cultivar Maksimirski rani with the strain Rhizobium leguminosarum bv. viciae 1001 influenced also higher milk production per hectare compared to the control and the nitrogen top-dressed variant.

  11. Fenologia da aceroleira cv. Olivier em Junqueirópolis-SP

    OpenAIRE

    Adriano,Elisa; Leonel,Sarita

    2012-01-01

    A aceroleira é uma planta de clima tropical que produz frutos muito ricos em vitamina C. Apesar de ser amplamente difundida, são poucos os trabalhos realizados com essa cultura no País. O presente trabalho objetivou avaliar a fenologia e o crescimento dos frutos da aceroleira cv. Olivier em duas épocas. Para tanto, foi implantado um experimento em pomar comercial no município de Junqueirópolis-SP. Foram avaliados dois ciclos reprodutivos: setembro de 2009 e janeiro de 2010, sendo que, em cada...

  12. Regeneration of plants from leaves of Chrysanthemum morifolium Ram. cv. Bronze Bornholm in in vitro cultures

    Directory of Open Access Journals (Sweden)

    Aurelia Ślusarkiewicz-Jarzina

    2014-01-01

    Full Text Available Plants were obtained from cultured in vitro leaves of Chrysanthemum morifolium Ram. cv. Bronze Bornholm. The leaves were inoculated on Murashige and Skoog medium (MS supplemented with cytokinins (kinetin - KIN, zeatin - ZEA, 6-benzyloaminopurine - BAP and auxins (2,4-dichlorophenoxyacetic acid - 2,4-D, α-naphtaleneacetic acid - NAA, 3-indolilacetic acid - IAA, p-fluorophenylalanine - PFA in various combinations and concentra-tions. The most suitable medium was that one which contained 4 mg/l KIN, 2 mg/l NAA and 50 mg/l PFA.

  13. Business Strategy of CV Jaya Sampurna in Facing Soft Drink Distributor Competition in Bekasi

    OpenAIRE

    Nurmala, Seri; Hartiwi, Hartiwi

    2015-01-01

    CV Jaya Sampurna is a soft drink distributor in Bekasi. Distributor company has a low profit margin, moreover the product sold is Fast Moving Consumers Goods with a very low profit margin (3.5%–5%). Therefore the company depends on a high volume of sales. Besides, there are many competitors in this industry, thus the business strategy is needed to improve the competitive advantage of the company. Given the conditions,this research was to identify the internal and the external factors, to iden...

  14. Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca

    Directory of Open Access Journals (Sweden)

    Glaucia Almeida de Morais

    1998-06-01

    Full Text Available Determination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.

  15. Análisis fitoquímico de frutos de Prunus persica cv Vesuvio

    OpenAIRE

    Muñoz Benito, Álvaro

    2014-01-01

    Los compuestos fenólicos desempeñan un papel importante en la calidad de los alimentos y contribuyen al aroma y sabor de la fruta. Además, han mostrado una importante actividad fisiológica en seres humanos como antioxidantes y agentes contra el cáncer. En este trabajo se ha iniciado el estudio fitoouirnico del fruto de Prunus oersice cv Vesuvio. una variedad de melocotón típica de la región de Campania, en el sur de Italia. Para ello la piel del melocotón deshidratada se somete...

  16. Herpes simplex virus produces larger plaques when assayed on ultraviolet irradiated CV1 cells

    International Nuclear Information System (INIS)

    Coohill, T.P.; Babich, M.A.; Taylor, W.D.; Snipes, W.

    1980-01-01

    Plaque development for either untreated or UV treated irradiated Herpes simplex virus Type 1 was faster when assayed on UV irradiated CV1 cells. This Large Plaque Effect only occurred if a minimum delay of 12h between cell irradiation and viral inoculation was allowed. Shorter delays gave plaques that were smaller than controls (unirradiated virus-unirradiated cells). The effect was maximal for a 48-h delay and remained unchanged for delays as long as 84h. The effect was greatest for cell exposures of 10Jm -2 . (author)

  17. Influence of gamma radiation on carbohydrates metabolism of ripening papaya (Carica papaya L. cv. Solo)

    International Nuclear Information System (INIS)

    Gomez, M.L.P.A.; Lajolo, F.M.; Cordenunsi, B.R.

    1999-01-01

    Food irradiation is one of the most promising treatments that can be utilized for fruits disinfestation and extension of shelf life. The authors studied the influence of 0,5 kGy of Gamma irradiation on the soluble carbohydrates composition of papaya (Carica papaya L. cv. Solo) fruit, and on sucrose metabolizing enzymes: sucrose synthase (SS), sucrose-phosphate synthase, acid and neutral invertases activities, during ripening. The results demonstrated that ethylene production, total soluble sugars, sucrose content, and sucrose-phosphate synthase and invertases activities were affected by irradiation, but not respiration, glucose and fructose content, and SS activity. (author)

  18. QUALIDADE DE GOIABAS CV. 'PALUMA' SUBMETIDAS A INJÚRIAS MECÂNICAS E FRIGOARMAZENAMENTO

    OpenAIRE

    HELTON DE SOUZA SILVA; RAILENE HÉRICA CARLOS ROCHA; FRANCISCO DE ASSIS DE SOUSA

    2013-01-01

    This work aimed to evaluate the effect of mechanical injuries caused by impact, abrasion and cut in guavas cv. 'Paluma' under fruit quality during cold storage at 10°C, and at environment temperature. Was to adopt the completely randomized design in a split-plot in time considering as plots, the mechanical injuries and subplots, the storage periods (0, 5, 8, 11, 14 and 17 days) with four replicates of two fruits per experimental unit. The mechanical injuries were induced considering the follo...

  19. Fruit quality of barbados cherry trees cv. Olivier in two stages of maturity

    OpenAIRE

    Adriano, Elisa; Leonel, Sarita; Evangelista, Regina Marta

    2011-01-01

    A acerola é um fruto de grande potencial econômico e nutricional devido ao seu alto teor de vitamina C, destacando-se como alimento funcional. É comercializada principalmente na forma de polpa congelada e fruto in natura. O objetivo deste estudo foi avaliar a qualidade dos frutos da aceroleira cv. Olivier em dois estádios de maturação. Para tanto, foram colhidas amostras de frutos em um pomar comercial no município de Junqueirópolis-SP. Foram realizadas a determinação da cor externa dos fruto...

  20. Chemical and ecological control methods for Epitrix spp.

    Directory of Open Access Journals (Sweden)

    A. G. S. Cuthbertson

    2015-01-01

    Full Text Available Very little information exists in regards to the control options available for potato flea beetles, Epitrix spp. This short review covers both chemical and ecological options currently available for control of Epitrix spp. Synthetic pyrethroids are the weapon of choice for the beetles. However, the impetus in integrated pest management is to do timely (early-season applications with something harsh which will give long-term protection at a time when there are not a lot of beneficials in the field. Finding the balance for control of Epitrix spp. is proving difficult.

  1. Interaction of Salmonella spp. with the intestinal microbiota

    Directory of Open Access Journals (Sweden)

    Brian MM Ahmer

    2011-05-01

    Full Text Available Salmonella spp. are major cause of human morbidity and mortality worldwide. Upon entry into the human host, Salmonella spp. must overcome the resistance to colonization mediated by the gut microbiota and the innate immune system. They successfully accomplish this by inducing inflammation and mechanisms of innate immune defense. Many models have been developed to study Salmonella spp. interaction with the microbiota that have helped to identify factors necessary to overcome colonization resistance and to mediate disease. Here we review the current state of studies into this important pathogen/microbiota/host interaction in the mammalian gastrointestinal tract.

  2. Occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system, Paraná, Southern Brazil

    Directory of Open Access Journals (Sweden)

    Jonatas Campos Almeida

    Full Text Available The purpose of this study was to investigate the occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system. Samples of raw and treated water were collected and concentrated using the membrane filtration technique. Direct Immunofluorescence Test was performed on the samples. DNA extraction using a commercial kit was performed and the DNA extracted was submitted to a nested-PCR reaction (n-PCR and sequencing. In the immunofluorescence, 2/24 (8.33% samples of raw water were positive for Giardia spp.. In n-PCR and sequencing, 2/24 (8.33% samples of raw water were positive for Giardia spp., and 2/24 (8.33% samples were positive for Cryptosporidium spp.. The sequencing showed Cryptosporidium parvum and Giardia duodenalis DNA. In raw water, there was moderate correlation among turbidity, color and Cryptosporidium spp. and between turbidity and Giardia spp.. The presence of these protozoans in the water indicates the need for monitoring for water-treatment companies.

  3. Distribution of aliphatic amines in CO, CV, and CK carbonaceous chondrites and relation to mineralogy and processing history

    Science.gov (United States)

    Aponte, José C.; Abreu, Neyda M.; Glavin, Daniel P.; Dworkin, Jason P.; Elsila, Jamie E.

    2017-12-01

    The analysis of water-soluble organic compounds in meteorites provides valuable insights into the prebiotic synthesis of organic matter and the processes that occurred during the formation of the solar system. We investigated the concentration of aliphatic monoamines present in hot acid water extracts of the unaltered Antarctic carbonaceous chondrites, Dominion Range (DOM) 08006 (CO3) and Miller Range (MIL) 05013 (CO3), and the thermally altered meteorites, Allende (CV3), LAP 02206 (CV3), GRA 06101 (CV3), Allan Hills (ALH) 85002 (CK4), and EET 92002 (CK5). We have also reviewed and assessed the petrologic characteristics of the meteorites studied here to evaluate the effects of asteroidal processing on the abundance and molecular distributions of monoamines. The CO3, CV3, CK4, and CK5 meteorites studied here contain total concentrations of amines ranging from 1.2 to 4.0 nmol g-1 of meteorite; these amounts are 1-3 orders of magnitude below those observed in carbonaceous chondrites from the CI, CM, and CR groups. The low-amine abundances for CV and CK chondrites may be related to their extensive degree of thermal metamorphism and/or to their low original amine content. Although the CO3 meteorites, DOM 08006 and MIL 05013, do not show signs of thermal and aqueous alteration, their monoamine contents are comparable to those observed in moderately/extensively thermally altered CV3, CK4, and CK5 carbonaceous chondrites. The low content of monoamines in pristine CO carbonaceous chondrites suggests that the initial amounts, and not asteroidal processes, play a dominant role in the content of monoamines in carbonaceous chondrites. The primary monoamines, methylamine, ethylamine, and n-propylamine constitute the most abundant amines in the CO3, CV3, CK4, and CK5 meteorites studied here. Contrary to the predominance of n-ω-amino acid isomers in CO3 and thermally altered meteorites, there appears to be no preference for the larger n-amines.

  4. Horses seropositive for Toxoplasma gondii, Sarcocystis spp. and Neospora spp.: Possible risk factors for infection in Brazil.

    Science.gov (United States)

    Cazarotto, Chrystian J; Balzan, Alexandre; Grosskopf, Rhayana K; Boito, Jhonatan P; Portella, Luiza P; Vogel, Fernanda F; Fávero, Juscivete F; de C Cucco, Diego; Biazus, Angelisa H; Machado, Gustavo; Da Silva, Aleksandro S

    2016-10-01

    Many parasitic diseases are considered asymptomatic, even though some studies have shown that they may cause pathological changes in the host. Therefore, the aim of this study was to evaluate the occurrence of antibodies against Toxoplasma gondii, Neospora spp. and Sarcocystis spp. in horses, and to identify the risk factors for disease. For this, 174 horses were studied, 90 males and 84 females aged between two and 20 years old. Blood samples were collected and stored in tubes without anticoagulant to obtain serum, which was subjected to serological tests for T. gondii, Sarcocystis spp., and Neospora spp. using indirect immunofluorescence assay (IFA). IFA results were as follows: Sarcocystis spp. 41.37% (72/174) (CI95%-34.05-49.09); T. gondii 32.18% (56/174) (CI95%-25.42-39.74) and Neospora spp. 48.27% (84/174) (CI95%-40.68.50-55.93). Out of 174 horses, 81 had simple infection, 61 had mixed infections with two or three of these pathogens, and therefore, only 32 horses showed no antibodies to any of these pathogens. No risk factors for Sarcocystis spp. and T. gondii infection were identified. However, there was a significant (1.22-CI95%-1.02-1.52) relationship between animal age and Neospora spp. infection, since older animals showed higher prevalence. Therefore, it was possible to conclude that T. gondii and Neospora spp. affect horses in Southern Brazil, however all the animals studied were asymptomatic without reproductive, neurological or locomotor problems. Copyright © 2016 Elsevier Ltd. All rights reserved.

  5. Comparative effects of artemisia vulgaris and charcoal moxa stimulating Zhongwan (CV 12) on body temperature in healthy participants: a cross-over single-blind randomized study.

    Science.gov (United States)

    Go, Ho-Yeon; Lee, Ju Ah; Park, Sunyoung; Park, Sunju; Park, Jeong-Su; Cheon, Chunhoo; Ko, Seong-Gyu; Kong, Kyung-Hwan; Jun, Chan-yong; Park, Jong-hyeong; Shin, Mi-Ran; Lee, Se-Hoon

    2015-10-01

    To evaluate the efficacy, safety, satisfaction, discomfort and patient preference of moxa cones of artemisia vulgaris and charcoal moxa. This comparative study of moxibustion treatment with Artemisia vulgaris and charcoal moxa cone stimulating Zhongwan (CV 12) is a cross-over single-blinded, randomized clinical trial. A total of 40 healthy subjects (24 males and 16 females) participated in this study. Two subjects dropped out of the trial. Thirty-eight subjects were treated with Artemisia vulgaris and charcoal moxa cones for 30 min in a cross-over design. After treatment, the patients underwent a 30 minute waiting period, and then the temperatures at Tanzhong (CV 17), Zhongwan (CV 12) and Guanyuan (CV 4) were measured using digital infrared thermal imaging. After the use of Artemisia vulgaris moxa, the patients' body temperatures were slightly lowered at Tanzhong (CV 17), Zhongwan (CV 12) and Guanyuan (CV 4), but the changes were not statistically significant. After the use of charcoal moxa, the patients' body temperatures were somewhat increased at Zhongwan (CV 12) and Guanyuan (CV 4), but the changes were not statistically significant. After Artemisia vulgaris moxa use, the body temperature difference between Zhongwan (CV 12) and Guanyuan (CV 4) was significantly increased. After charcoal moxa use, the body temperature difference between Tanzhong (CV 17) and Zhongwan (CV 12) was significantly decreased in males and in the whole group. This change was caused by the difference in the moxibustion type and by gender differences. This pilot study found that moxibustion did not raise the body temperature, but temperature differences between acupoints were affected. Further large-scale randomized controlled trials are needed for the effect of moxibustion on body temperature.

  6. ORGANIC FERTILIZER AS PHOSPHORUS SUPPLIER FOR Brachiaria brizantha CV. MARANDÚ FERTILIZANTE ORGÂNICO COMO FONTE DE FÓSFORO NO CULTIVO INICIAL DE Brachiaria brizantha CV. MARANDÚ

    Directory of Open Access Journals (Sweden)

    Diego Henriques Santos

    2010-05-01

    Full Text Available

    Phosphorus is considered an essential element for plants, but it is found in low amounts in Brazilian soils, mainly in areas destined to pastures, and the application of organic matter contributes to the improvement of physical, chemical and biological soil attributes. The research had the objective of evaluating the application effects of the Bokashi organic compound on soil macro and micronutrients contents and leaves, as well as on Brachiaria brizantha cv. Marandú dry mass yield, evaluating the potential of the organic fertilizer as phosphorus source, in comparison with conventional chemical fertilizers. Different organic compound doses supplied the soil with 17 kg P2O5 ha-1, 34 kg P2O5 ha-1, 68 kg P2O5 ha-1, 136 kg P2O5 ha-1, and 204 kg P2O5 ha-1, comparing it with the conventional chemical fertilizer and the control. The results revealed that the organic fertilizer Bokashi can adequately replace the conventional chemical fertilizer of Brachiaria brizantha cv. Marandú, supplying the soil and plant with appropriate nutrients levels and maintaining the dry mass yield.

    KEY-WORDS: Organic matter; organic fertilizers; plants mineral nutrition; Bokashi

  7. Biosynthesized Silver Nanoparticles Used in Preservative Solutions for Chrysanthemum cv. Puma

    Directory of Open Access Journals (Sweden)

    Luis M. Carrillo-López

    2016-01-01

    Full Text Available The use of pulse solutions containing antimicrobials has been reported, but more research is necessary. To increase vase life and to study their effect on opening inflorescences, silver nanoparticles were used in vase solutions for cv. Puma Chrysanthemum stems. The nanoparticles were synthesized biologically using Chenopodium ambrosioides L. applied at concentrations of 0.01, 0.05, 0.1, 0.5, 1, and 5 mM and compared with a control. Treatments were replicated five times. The stems were cut to 50 cm and observed until the end of their vase life. Low concentrations of silver nanoparticles promoted inflorescence opening and leaf yellowing, while the control leaves remained green, but there was a lower degree of inflorescence opening. High concentrations of silver nanoparticles (0.5, 1, and 5 mM caused senescence due to low water uptake through the stems. Statistical differences in inflorescence opening and diameter, bacterial growth (CFU mL−1 in vase solutions, fresh weight, water uptake, and vase life were found among treatments. Longer vase life and less weight loss were observed in the stems exposed to low concentrations of silver nanoparticles. Low concentrations of silver nanoparticles promoted inflorescence opening and increased vase life of Chrysanthemum cv. Puma.

  8. Determination of polyphenols and antioxidant activity of Vitis labrusca cv. baile berries.

    Science.gov (United States)

    Nile, Shivraj Hariram; Park, Se Won

    2015-10-01

    Grape juice and grape skin extracts are important commercial source of polyphenolic compounds which exert different functional properties such as color potential, antimicrobial, antioxidant activity, and health benefits. In this paper we describe a sensitive and specific assay for determination of bioactive polyphenolic compounds in Campbell Early (Vitis labrusca cv. baile). Five polyphenolic components were separated on an Agilent Zorbax Extend C18 Column (250 mm x 4.6 mm x 5 μm) and detected by a diode array detector. The mobile phase was composed of (a) aqueous phosphoric acid (0.2%, v/v); and (b) acetonitrile using a gradient elution. Analytes were performed at 25 degrees C with a flow rate of 0.8 ml/min and UV detection at 280, 360, and 520 nm. All calibration curves showed good linear regression (r2 ≥ 0.9999) within tested ranges. Overall intra- and inter-day variations were less than 1.90%, and the average recoveries were 95.5-105% for analytes. The antioxidant activity determined by DPPH radical assay, ranged from 86-105 for extracts, and 165-252 for studied standards (μM trolox/100 g dry wt.). The proposed method would be sensitive enough and reliable for quality control in functional food and modernization of Campbell Early (Vitis labrusca cv. baile) as potent antioxidant agents.

  9. Preliminary results of the pCT scanner testing at CV-28

    Energy Technology Data Exchange (ETDEWEB)

    Setti, Joao A.P.; Schelin, Hugo R.; Paschuk, Sergei A.; Milhoretto, Edney; Rocha, Rodrigo L. [Federal University of Technology (UTFPR), Curitiba, PR (Brazil)], E-mail: setti@utfpr.edu.br, E-mail: schelin@cpgei.cefetpr.br, E-mail: sergei@utfpr.edu.brj, E-mail: edneymilhoretto@yahoo.com, E-mail: rodrigo.luis.rocha@gmail.com; Ribeiro Junior, Sebastiao [Instituto de Tecnologia para o Desenvolvimento (LACTEC), Curitiba, PR (Brazil)], E-mail: ribeiro@lactec.org.br; Evseev, Ivan G.; Assis, Joaquim T. de; Yevseyeva, Olga [Universidade do Estado do Rio de Janeiro (UERJ), Nova Friburgo, RJ (Brazil). Inst. Politecnico], E-mail: evseev@iprj.uerj.br, E-mail: joaquim@iprj.uerj.br, E-mail: yevseyeva@iprj.uerj.br; Lopes, Ricardo T. [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Coordenacao dos Programas de Pos-graduacao de Engenharia (COPPE). Lab. de Instrumentacao Nuclear], E-mail: ricardo@lin.ufrj.br; Vinagre Filho, Ubirajara M. [Instituto de Engenharia Nuclear IEN/CNEN-RJ, Rio de Janeiro, RJ (Brazil)], E-mail: bira@ien.gov.br

    2007-07-01

    In the present work the first results obtained with the low energy proton beam of CV-28 at IEN/CNEN and the prototype of a computerized tomography device developed in UFTPR are presented. The system installed in the scatterings chamber of the cyclotron line 3 includes the proton scatter, turntable with translation (1st generation CT scheme), the set of collimators for the secondary proton beam formation and the Si(Li) ORTEC detector. The main parameters of the tomography turntable and the collimators were chosen based on computer simulations with SRIM2006 and GEANT4. A cylindrical glass tube was used as the irradiated sample. On this stage, only the translation of the turntable perpendicular to the proton beam direction was fulfilled. The measured proton energy spectra have, in general, a predicted behavior. However, the experiment revealed some problems with the secondary proton beam formation that should be solved prior to obtain a first pCT image at CV-28. (author)

  10. Production and quality of mini watermelon cv. Smile irrigated with saline water

    Directory of Open Access Journals (Sweden)

    Alan B. O. de Sousa

    Full Text Available ABSTRACT The purpose of this study was to evaluate the salt tolerance of mini watermelon (cv. Smile. The experiment was carried out in the greenhouse of the Department of Biosystems Engineering of ESALQ/USP. The experimental design was randomized blocks. The plants were irrigated with five levels of salinity (S1 = 1.0; S2 = 2.0; S3 = 3.0; S4 = 4.0 and S5 = 5.0 dS m-1. At 85 days after the beginning of the experiment, the plants and the physical and chemical variables of the fruit were evaluated. Salinity negatively affected the variables: length of the main stem, stem diameter, number of leaves, number of branches, leaf area, fresh and dry matter. Regarding the physical and chemical characteristics of fruits, salinity reduced the mass, diameter and the pH of the fruit, but increased its vitamin C content. In general, the results suggest that the mini watermelon (cv. Smile is moderately sensitive to salinity.

  11. Slurry sampling in serum blood for mercury determination by CV-AFS

    Energy Technology Data Exchange (ETDEWEB)

    Aranda, Pedro R. [Departmento de Quimica Analitica, Facultad de Quimica, Bioquimica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, P.O. Box 375, 5700 San Luis (Argentina); Instituto de Quimica San Luis (INQUISAL-CONICET), Chacabuco y Pedernera, P.O. Box 375, 5700 San Luis (Argentina)], E-mail: paranda@unsl.edu.ar; Gil, Raul A. [Departmento de Quimica Analitica, Facultad de Quimica, Bioquimica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, P.O. Box 375, 5700 San Luis (Argentina); Instituto de Quimica San Luis (INQUISAL-CONICET), Chacabuco y Pedernera, P.O. Box 375, 5700 San Luis (Argentina)], E-mail: ragil@unsl.edu.ar; Moyano, Susana [Departmento de Quimica Analitica, Facultad de Quimica, Bioquimica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, P.O. Box 375, 5700 San Luis (Argentina)], E-mail: smoyano@unsl.edu.ar; De Vito, Irma [Departmento de Quimica Analitica, Facultad de Quimica, Bioquimica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, P.O. Box 375, 5700 San Luis (Argentina)], E-mail: devito@unsl.edu.ar; Martinez, Luis D. [Departmento de Quimica Analitica, Facultad de Quimica, Bioquimica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, P.O. Box 375, 5700 San Luis (Argentina); Instituto de Quimica San Luis (INQUISAL-CONICET), Chacabuco y Pedernera, P.O. Box 375, 5700 San Luis (Argentina)], E-mail: ldm@unsl.edu.ar

    2009-01-30

    The heavy metal mercury (Hg) is a neurotoxin known to have a serious health impact even at relatively low concentrations. A slurry method was developed for the sensitive and precise determination of mercury in human serum blood samples by cold vapor generation coupled to atomic fluorescence spectrometry (CV-AFS). All variables related to the slurry formation were studied. The optimal hydrochloric concentration and tin(II) chloride concentration for CV generation were evaluated. Calibration within the range 0.1-10 {mu}g L{sup -1} Hg was performed with the standard addition method, and compared with an external calibration. Additionally, the reliability of the results obtained was evaluated by analyzing mercury in the same samples, but submitted to microwave-assisted digestion method. The limit of detection was calculated as 25 ng L{sup -1} and the relative standard deviation was 3.9% at levels around of 0.4 {mu}g L{sup -1} Hg.

  12. Intestinal Permeability and Cellular Antioxidant Activity of Phenolic Compounds from Mango (Mangifera indica cv. Ataulfo) Peels.

    Science.gov (United States)

    Pacheco-Ordaz, Ramón; Antunes-Ricardo, Marilena; Gutiérrez-Uribe, Janet A; González-Aguilar, Gustavo A

    2018-02-08

    Mango ( Mangifera indica cv. Ataulfo) peel contains bound phenolics that may be released by alkaline or acid hydrolysis and may be converted into less complex molecules. Free phenolics from mango cv. Ataulfo peel were obtained using a methanolic extraction, and their cellular antioxidant activity (CAA) and permeability were compared to those obtained for bound phenolics released by alkaline or acid hydrolysis. Gallic acid was found as a simple phenolic acid after alkaline hydrolysis along with mangiferin isomers and quercetin as aglycone and glycosides. Only gallic acid, ethyl gallate, mangiferin, and quercetin were identified in the acid fraction. The acid and alkaline fractions showed the highest CAA (60.5% and 51.5%) when tested at 125 µg/mL. The value of the apparent permeability coefficient (Papp) across the Caco-2/HT-29 monolayer of gallic acid from the alkaline fraction was higher (2.61 × 10 -6 cm/s) than in the other fractions and similar to that obtained when tested pure (2.48 × 10 -6 cm/s). In conclusion, mango peels contain bound phenolic compounds that, after their release, have permeability similar to pure compounds and exert an important CAA. This finding can be applied in the development of nutraceuticals using this important by-product from the mango processing industry.

  13. Business Strategy of CV Jaya Sampurna in Facing Soft Drink Distributor Competition in Bekasi

    Directory of Open Access Journals (Sweden)

    Seri Nurmala

    2015-11-01

    Full Text Available CV Jaya Sampurna is a soft drink distributor in Bekasi. Distributor company has a low profit margin, moreover the product sold is Fast Moving Consumers Goods with a very low profit margin (3.5%–5%. Therefore the company depends on a high volume of sales. Besides, there are many competitors in this industry, thus the business strategy is needed to improve the competitive advantage of the company. Given the conditions,this research was to identify the internal and the external factors, to identify the formulation of business strategy alternative, and to identify the recommendation of functional strategy for CV Jaya Sampurna. Research used descriptive method; and the type of research used was case study. Data were gathered from questionnaires and interview to three member of managers: Director, Operational Manager, and Finance Manager. Data wereanalyzed by using EFE, IFE, SWOT, IE, Grand Strategy Matrix and QSPM. The result of this research implies that the alternative strategy based on QSPM’s result was market penetration

  14. C-V characterization of Schottky- and MIS-gate SiGe/Si HEMT structures

    International Nuclear Information System (INIS)

    Onojima, Norio; Kasamatsu, Akihumi; Hirose, Nobumitsu; Mimura, Takashi; Matsui, Toshiaki

    2008-01-01

    Electrical properties of Schottky- and metal-insulator-semiconductor (MIS)-gate SiGe/Si high electron mobility transistors (HEMTs) were investigated with capacitance-voltage (C-V) measurements. The MIS-gate HEMT structure was fabricated using a SiN gate insulator formed by catalytic chemical vapor deposition (Cat-CVD). The Cat-CVD SiN thin film (5 nm) was found to be an effective gate insulator with good gate controllability and dielectric properties. We previously investigated device characteristics of sub-100-nm-gate-length Schottky- and MIS-gate HEMTs, and reported that the MIS-gate device had larger maximum drain current density and transconductance (g m ) than the Schottky-gate device. The radio frequency (RF) measurement of the MIS-gate device, however, showed a relatively lower current gain cutoff frequency f T compared with that of the Schottky-gate device. In this study, C-V characterization of the MIS-gate HEMT structure demonstrated that two electron transport channels existed, one at the SiGe/Si buried channel and the other at the SiN/Si surface channel

  15. a Fast Segmentation Algorithm for C-V Model Based on Exponential Image Sequence Generation

    Science.gov (United States)

    Hu, J.; Lu, L.; Xu, J.; Zhang, J.

    2017-09-01

    For the island coastline segmentation, a fast segmentation algorithm for C-V model method based on exponential image sequence generation is proposed in this paper. The exponential multi-scale C-V model with level set inheritance and boundary inheritance is developed. The main research contributions are as follows: 1) the problems of the "holes" and "gaps" are solved when extraction coastline through the small scale shrinkage, low-pass filtering and area sorting of region. 2) the initial value of SDF (Signal Distance Function) and the level set are given by Otsu segmentation based on the difference of reflection SAR on land and sea, which are finely close to the coastline. 3) the computational complexity of continuous transition are successfully reduced between the different scales by the SDF and of level set inheritance. Experiment results show that the method accelerates the acquisition of initial level set formation, shortens the time of the extraction of coastline, at the same time, removes the non-coastline body part and improves the identification precision of the main body coastline, which automates the process of coastline segmentation.

  16. QUALIDADE DE GOIABAS CV. 'PALUMA' SUBMETIDAS A INJÚRIAS MECÂNICAS E FRIGOARMAZENAMENTO

    Directory of Open Access Journals (Sweden)

    HELTON DE SOUZA SILVA

    2013-01-01

    Full Text Available This work aimed to evaluate the effect of mechanical injuries caused by impact, abrasion and cut in guavas cv. 'Paluma' under fruit quality during cold storage at 10°C, and at environment temperature. Was to adopt the completely randomized design in a split-plot in time considering as plots, the mechanical injuries and subplots, the storage periods (0, 5, 8, 11, 14 and 17 days with four replicates of two fruits per experimental unit. The mechanical injuries were induced considering the following criteria: T1, witness, without fruit visible damage; T2, fruits impacts, two impacts per fruit at a height of 85 cm; T3, fruits subjected two cuts, on opposite sides, depth of 2 mm and length 30 mm; T4, fruits subjected to abrasion, area of 10 cm2. Was evaluated the exterior color, the external appearance, the internal appearance, the severity of disease. Injury due to impact, abrasion and cut no influence on in exterior color, external appearance, internal appearance and severity of diseases of guava cv. 'Paluma' during storage for 11 days at 10oC. But, the quality was impaired by mechanical injuries six days after exposure to 30°C.

  17. Quality characteristics of soybean pasted (Doenjang) manufactured with 2 soybean mutant lines derived from cv. baekwon

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Kyung Jun; Kang, Si Yong; Choi, Hong Il; Kim, Jin Baek [Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup (Korea, Republic of)

    2016-11-15

    In order to identification of the possibility of manufacturing soybean paste (doenjang) with soybean mutant lines induced from gamma-ray mutagenesis, this study was performed to investigate the quality characteristics of doenjang using two soybean mutant lines, Baekwon-1 (BW-1) and Baekwon-2 (BW-2) and their original cultivar (cv. Baekwon, BW) for 8 weeks. The BW and two mutant lines (BW-1 and BW-2) were showed higher content of amino type nitrogen than control (cv. Taegwang). The pH decreased and the titratable acidity increased all the samples during aging period. The lightness, redness and yellowness of doenjang were the lowest in BW. Total free sugar content of doenjang was the highest in control (10.43%) after 4 weeks and composed mainly fructose and glucose. The order of the free amino acid content was Glutamic acid>Leucine>Lysine>Phenylalanine>Aspartic acid in control, Glutamic acid>Leucine >Arginine>Lysine>Phenylalanine in BW, Glutamic acid>Lysine>Phenylalanine>Aspartic acid>Valine in BW-1 and Glutamic acid>Arginine>Lysine>Phenylalanine>Aspartic acid in BW-2, respectively. Our results showed that it is possible to increase the quality of doenjang using soybean mutant lines in manufacturing soybean paste.

  18. Le bureau d'études TS/CV/BE pour les besoins du projet LHC

    CERN Document Server

    Martel, C

    2004-01-01

    Le bureau d'études TS/CV/BE propose un appui technique personnalisé aux différentes sections du groupe TS/CV, responsables des projets liés aussi bien à la machine LHC, ses zones expérimentales, que d'autres projets associés. L'équipe, constituée historiquement et exclusivement de personnel d'entreprise extérieure, dispose d'un effectif variable, adapté à des besoins planifiés. Le choix d'utiliser les mêmes logiciels de CAO que la communauté du CERN, a limité la proportion de spécialistes au sein de l'équipe. Une méthodologie particulière a donc été mise en place pour satisfaire les demandes, tout en faisant face à l'étendue et à la complexité des installations de ventilation et de refroidissement. Des compétences externes spécialisées sont également sollicitées, au cas par cas, pour optimiser les coûts de fonctionnement.

  19. A FAST SEGMENTATION ALGORITHM FOR C-V MODEL BASED ON EXPONENTIAL IMAGE SEQUENCE GENERATION

    Directory of Open Access Journals (Sweden)

    J. Hu

    2017-09-01

    Full Text Available For the island coastline segmentation, a fast segmentation algorithm for C-V model method based on exponential image sequence generation is proposed in this paper. The exponential multi-scale C-V model with level set inheritance and boundary inheritance is developed. The main research contributions are as follows: 1 the problems of the "holes" and "gaps" are solved when extraction coastline through the small scale shrinkage, low-pass filtering and area sorting of region. 2 the initial value of SDF (Signal Distance Function and the level set are given by Otsu segmentation based on the difference of reflection SAR on land and sea, which are finely close to the coastline. 3 the computational complexity of continuous transition are successfully reduced between the different scales by the SDF and of level set inheritance. Experiment results show that the method accelerates the acquisition of initial level set formation, shortens the time of the extraction of coastline, at the same time, removes the non-coastline body part and improves the identification precision of the main body coastline, which automates the process of coastline segmentation.

  20. Somatic embryogenesis from bud and leaf explants of date palm (Phoenix dactylifera L.) cv. Najda.

    Science.gov (United States)

    Mazri, Mouaad Amine; Belkoura, Ilham; Meziani, Reda; Mokhless, Boutaïna; Nour, Souad

    2017-05-01

    An efficient regeneration system through somatic embryogenesis was developed for date palm cv. Najda. Adventitious bud and proximal leaf segments cultured on Murashige and Skoog (MS) medium supplemented with various combinations of auxins and cytokinins induced embryogenesis after at least 6 months of culture. Somatic embryogenesis induction seemed correlated with the type of the explant, the induction period and the auxin used. The highest rate of somatic embryogenesis (86.0%) was obtained on bud explants cultured on MS medium supplemented with 45.0 µM 2,4-dichlorophenoxyacetic acid (2,4-D), and 4.5 µM kinetin or 4.5 µM 6-(dimethylallylamino) purine (2iP). Whereas, low levels of embryogenesis were obtained on media supplemented with 1-naphthalene acetic acid (NAA) or 2-naphthoxyacetic acid (NOA). Proximal leaf segments showed somatic embryogenesis only when cultured on media supplemented with 2,4-D or picloram. Statistical analysis revealed significant effects of explant type and plant growth regulators (PGRs) combination on somatic embryogenesis. Somatic embryos were germinated successfully on PGR-free MS medium with or without activated charcoal (50.0-60.0 and 26.6-36.6%, respectively), and 80.0% of plantlets survived after transferring to a glasshouse for 6 months. Our results will be useful for large-scale propagation of date palm cv. Najda, characterized by high fruit quality and bayoud disease resistance.

  1. Exploring the SDSS Dataset with Linked Scatter Plots: I. EMP, CEMP, and CV Stars

    Science.gov (United States)

    Carbon, Duane F.; Henze, Christopher; Nelson, Bron C.

    2017-01-01

    We present the results of a search for EMP, CEMP, and cataclysmic variable stars using a new exploration tool based on linked scatter plots (LSPs). Our approach is especially designed to work with very large spectrum data sets such as the SDSS, LAMOST, RAVE, and Gaia data sets and can be applied to stellar, galaxy, and quasar spectra. As a demonstration, we conduct a search for EMP, CEMP, and cataclysmic variable stars in the SDSS DR10 data set. We first created a 3326-dimensional phase space containing nearly 2 billion measures of the strengths of over 1600 spectral features in 569,738 SDSS stars. These measures capture essentially all the stellar atomic and molecular species visible at the resolution of SDSS spectra. We show how LSPs can be used to quickly isolate and examine interesting portions of this phase space. To illustrate, we use LSPs coupled with cuts in selected portions of phase space to extract EMP stars, C-rich EMP stars, and CV stars. We present identifications for 59 previously unrecognized candidate EMP stars and 11 previously unrecognized candidate CEMP stars. We also call attention to 2 candidate He II emission CV stars found by the LSP approach that have not yet been discussed in the literature.

  2. PENINGKATAN EFEKTIFITAS DAN EFISIENSI BIAYA KUALITAS MELALUI PENEDEKATAN SIMULASI (Studi Kasus di CV. SINAR BAJA ELEKTRIC

    Directory of Open Access Journals (Sweden)

    I Gede Agus Widyadana

    2003-01-01

    Full Text Available The paper discusses about how to increase product quality and still concern with quality cost. We add factor of time to count quality cost and the effect to quality product. Time has important function in this research, so simulation is used to analyze quality control system. The simulation on CV. Sinar Baja Elektrik shows that the propose method can decrease product reject about 8% and quality cost until 16,89% from total sales. Abstract in Bahasa Indonesia : Makalah ini membahas mengenai upaya untuk meningkatkan kualitas produk dengan memperhatikan biaya kualitas. Penelitian ini memasukan tambahan faktor waktu dalam memperhitungkan biaya kualitas serta dampaknya pada kualitas produk. Oleh karena faktor waktu memegang peranan yang penting, maka analisa sistem pengendalian kualitas pada penelitian ini menggunakan metode simulasi. Hasil simulasi di CV. Sinar Baja Elektrik menunjukan metode yang diusulkan dapat menurunkan hasil rata-rata tingkat kelolosan produk cacat untuk tiap tipe speaker hingga berkisar 8%, dan total biaya kualitas secara keseluruhan dapat diturunkan hingga sebesar 16.89% dari total penjualan. Kata kunci: biaya kualitas, efisiensi, efektivitas, simulasi.

  3. Somatic embryos formation from immature cotyledons in Phaseolus vulgaris cv. CIAP 7247

    Directory of Open Access Journals (Sweden)

    Raúl Collado

    2011-10-01

    Full Text Available Common bean, like most of legumes, is recalcitrant to in vitro culture, so the establishment of a regeneration procedure via somatic embryogenesis in this specie has not been possible. Therefore, determining the effect of factors that influencing on somatic embryos formation constitutes an essential step to develop a regeneration protocol via somatic embryogenesis in common bean. Cotyledons of immature seeds were used as initial plant material to achieve the somatic embryos formation in Phaseolus vulgaris cv. CIAP 7247. Five 2,4-D concentrations (10, 20, 30, 40 y 50 mg l-1 and cotyledon orientation on culture medium were studied. After eight weeks on culture, the number of explants that formed somatic embryos and the number of somatic embryos per explant were evaluated. The results demonstrated that the major number of explants that formed somatic embryos and the number of somatic embryos formed per explant were obtained with 40 y 50 mg l-1 of 2,4-D. Somatic embryos were formed on abaxial side from the cotyledons when this side was placed opposite to the culture medium. Somatic embryos on cotyledons from immature seeds of Phaseolus vulgaris L. cv. CIAP 7247 were formed. It was demonstrated that the addition of 2,4-D into culture medium, and the explant orientation had a determining effect on somatic embryogenesis in this important legume. Key words: common bean, regeneration, somatic embryogenesis

  4. Morphogenic and structural traits of Brachiaria brizantha cv. Marandu under different nitrogen levels and residues heights

    Directory of Open Access Journals (Sweden)

    Eleuza Clarete Junqueira de Sales

    2014-10-01

    Full Text Available The objective of this study was to evaluate the rate of leaf appearance, leaf elongation rate, number of green leaves, length of blade and stem of Brachiaria brizantha cv. Marandu under different nitrogen levels and two residue heights. A randomized block design was used in a 4 x 2 factorial scheme, four doses of nitrogen (100, 200, 300 and 400 kg N ha-1 year-1 and two residue heights (5 and 15 cm. There was significant interaction between residue height and nitrogen levels for leaf appearance rate (P> 0.05 of signal grass, which was influenced negatively and linearly (P 0.05 between the levels of nitrogen x residue height on the number of green leaves. The dosage that provided the highest stalk in the residue height of 5 cm was of 271.5 kg N ha -1. An increase of 0.0745 cm tiller -1 day-1 for 1 kg of N ha -1 applied to height of 15 cm of residue was observed. Nitrogen fertilization contributes positively to growth and development of rates of appearance and leaf extension. The management of pasture of Brachiaria brizantha cv. Marandu with 5 cm of residue and fertilization with 100 kg N ha -1 provided better answer morphogenic traits.

  5. Storage and methyl jasmonate in postharvest conservation of roses cv. Avalanche

    Directory of Open Access Journals (Sweden)

    Elka Fabiana Aparecida Almeida

    2017-07-01

    Full Text Available The use of methyl jasmonate has demonstrated its efficiency to extend the vase life of cut flowers. The objective of this study was to evaluate the effect of methyl jasmonate associated with storage at low temperatures on the postharvest quality of Rosa cv. Avalanche stems. The treatments consisted of 125, 250, 500 and 1000 μM of methyl jasmonate, besides the control with distilled water. The flower buds were sprayed with 4 mL of the solution, according to the treatments, and then kept in a cold chamber (1 °C for periods of 2 and 6 days. Subsequently, the stems were taken to the postharvest laboratory at a temperature of 16 °C. Better quality, higher fresh weight and water absorption were observed in flower stems stored for 2 days. The application of methyl jasmonate caused less turgescence and greater darkening of roses. It was possible to conclude that two days is the best storage time at 1 °C and the use of methyl jasmonate does not maintain the quality of roses cv. Avalanche after harvest.

  6. Response of African eggplants to Fusarium spp. and identification of ...

    African Journals Online (AJOL)

    Response of African eggplants to Fusarium spp. and identification of sources of resistance. Phoebe Kirigo Mwaniki, Mathew Musumbale Abang, Isabel Nyokabi Wagara, Joseph Ngwela Wolukau, Schroers Hans-Josef ...

  7. Physiology and immunology of mucosal barriers in catfish (Ictalurus spp.)

    Science.gov (United States)

    The mucosal barriers of catfish (Ictalurus spp.) constitute the first line of defense against pathogen invasion while simultaneously carrying out a diverse array of other critical physiological processes, including nutrient adsorption, osmoregulation, waste excretion, and environmental sensing. Catf...

  8. Symbiotic relationship of Thiothrix spp. with an echinoderm

    Energy Technology Data Exchange (ETDEWEB)

    Brigmon, R.L. [Westinghouse Savannah River Co., Aiken, SC (United States); De Ridder, C. [Univ. Libre de Bruxelles, Brussels (Belgium). Lab. de Biologie Marine

    1998-09-01

    Thiothrix-like bacteria have been reported as symbionts in invertebrates from sulfide-rich habitats. Isolation of these symbiotic Thiothrix-like bacteria has failed, and the organisms have not been previously identified with certainty. The genus Thiothrix was created for ensheathed filamentous bacteria that oxidize sulfide and deposit sulfur granules internally, attach to substrates, produce gliding gonidia, and form rosettes. Immunoassay procedures were used to investigate the symbiotic relationship of Thiothrix spp. in the intestinal cecum of the spatangoid species Echinocardium cordatum. Thiothrix spp. were identified in nodule samples from E. cordatum digestive tubes based on microscopic examination, enzyme-linked immunosorbent assay, and indirect immunofluorescence. Thiothrix spp. protein made up as much as 84% of the total protein content of the nodules. This is the first identification of Thiothrix spp. internally symbiotic with marine invertebrates.

  9. Interlaboratorium vergelijking van het onderzoek naar Aeromonas spp. in drinkwater

    NARCIS (Netherlands)

    Havelaar AH; During M; Versteegh JFM

    1986-01-01

    Door middel van onderzoek van zes kunstmatig besmette gesimuleerde monsters drinkwater ( 4 Aeromonas spp., 1 Klebsiella oxytoca, 1 Pseudomonas aeruginosa) werd een vergelijking gemaakt van de resultaten van Aeromonas onderzoek in 14 laboratoria. De tellingen in de deelnemende laboratoria

  10. Application of the biosurfactants produced by Bacillus spp. (SH 20 ...

    African Journals Online (AJOL)

    Application of the biosurfactants produced by Bacillus spp. (SH 20 and SH 26) and P. aeruginosa SH 29 isolated from the rhizosphere soil of an Egyptian salt marsh plant for the cleaning of oil - contaminataed vessels and enhancing the biodegradat.

  11. Comparative Genomic Analysis of Holospora spp., Intranuclear Symbionts of Paramecia

    Directory of Open Access Journals (Sweden)

    Sofya K. Garushyants

    2018-04-01

    Full Text Available While most endosymbiotic bacteria are transmitted only vertically, Holospora spp., an alphaproteobacterium from the Rickettsiales order, can desert its host and invade a new one. All bacteria from the genus Holospora are intranuclear symbionts of ciliates Paramecium spp. with strict species and nuclear specificity. Comparative metabolic reconstruction based on the newly sequenced genome of Holospora curviuscula, a macronuclear symbiont of Paramecium bursaria, and known genomes of other Holospora species shows that even though all Holospora spp. can persist outside the host, they cannot synthesize most of the essential small molecules, such as amino acids, and lack some central energy metabolic pathways, including glycolysis and the citric acid cycle. As the main energy source, Holospora spp. likely rely on nucleotides pirated from the host. Holospora-specific genes absent from other Rickettsiales are possibly involved in the lifestyle switch from the infectious to the reproductive form and in cell invasion.

  12. Studies on Thiobacilli spp. isolated from sandy beaches of Kerala

    Digital Repository Service at National Institute of Oceanography (India)

    Gore, P.S.; Raveendran, O.; Unnithan, R.V.

    Occurrence, isolation and oxidative activity of Thiobacilli spp. from some sandy beaches of Kerala are reported. These organisms were encountered in polluted beaches and were dominant during monsoon in all the beaches...

  13. Nepenthes rafflesiana pitcher liquid has antifungal activity against Candida spp.

    OpenAIRE

    Hanna Yolanda; Ingrid M. Makahinda; Maureen Aprilia; Nikki Sanjaya; Harry Gunawan; Rita Dewi

    2015-01-01

    BACKGROUND To develop new effective antifungals, it is essential to search for antifungal compounds from plants such as Nepenthes spp., which have their greatest diversity in Indonesia. Since chitin-induced liquid (CIL) from Nepenthes khasiana pitchers has antifungal activity, due to their naphthoquinone content, this study aimed to evaluate antifungal activity of Nepenthes rafflesiana pitcher liquids on Candida spp. METHODS Collected pitcher liquids were of 3 types: non-induced l...

  14. Isolation of Cronobacter spp. (Enterobacter sakazakii from artisanal mozzarella

    Directory of Open Access Journals (Sweden)

    Francesco Casalinuovo

    2014-02-01

    Full Text Available Cronobacter spp. (Enterobacter sakazakii is an opportunistic bacterial pathogen capable of causing disease and even fatalities in newborn infants within the first weeks of life if consumed as part of the diet. Premature and immunocompromised newborn infants are at particular risk. The microorganism has been isolated from a variety of foods including contaminated infant milk formula powder and milk powder substitute. The study aimed to evaluate the level of microbiological contamination in 47 samples of mozzarella cheese made with cow’s milk collected from artisan cheese producers in Southern Italy. Samples were collected from commercial sales points and underwent qualitative and quantitative microbiological analyses to test for the bacterial contaminants most commonly found in milk and cheese products. The 47 samples underwent qualitative and quantitative microbiological tests according to ISO UNI EN standards. Analyses focused on Staphylococcus aures, Salmonella spp., Listeria monocytogenes, Pseudomonas spp., E. coli, Yersinia spp., total coliforms and Cronobacter sakazakii. The ISO/TS 22964:2006 method was used to investigate possible contamination by C. sakazakii. Biochemical identification was carried out using an automated system for identification and susceptibility tests. None of the samples examined resulted positive for Salmonella spp. or Listeria spp. Only one sample resulted positive for Staphylococcus aureus. Pseudomonas spp. was isolated in 10 (21% of 47 samples. High levels of total coliforms were found in 10 of 47 samples. Cronobacter spp. (Enterobacter sakazakii was isolated in one sample. This is the first study to confirm isolation of C. sakazakii in artisan mozzarella cheese made from cow’s milk. The presence of C. sakazakii could be related to external contamination during the phases of production or to the use of contaminated milk. Since mozzarella is recommended in the diet of children and adults of all ages, this

  15. Phenotypic characterization of canine Malassezia spp., isolates

    Directory of Open Access Journals (Sweden)

    Angélica Hurtado-Suárez

    2016-09-01

    Full Text Available Objective. To characterize and identify yeasts of the genus Malassezia by phenotypic features. Materials and methods. First, the macroscopic and microscopic morphological characteristics were described. In addition we performed biochemical and physiological assays as Tweens and Cremophor, including more. Results. Our results evidenced of 105 isolates obtained from dogs diagnosed with external otitis, it was possible to identify two distinct species from 46 isolates within the Malassezia genus: 36.19% (n=38 were identified as M. pachydermatis and 7.62% (n=8 as M. furfur. According to phenotypic patterns the remaining 56.19% (n=59 were reported as Malassezia spp., possibly corresponding to M. furfur and/or M. pachydermatis. Conclusions. Results emphasize the necessity to characterize according to species. It is not feasible to define Malassezia by species based on morphological, biochemical, and physiological findings. Therefore, molecular genotyping should be performed to identify markers allowing a more precise isolate identification. This would broaden our epidemiological knowledge regarding different species involved in canine otitis pathologies.

  16. Biopharmaceutical potentials of Prosopis spp. (Mimosaceae, Leguminosa

    Directory of Open Access Journals (Sweden)

    Santhaseelan Henciya

    2017-01-01

    Full Text Available Prosopis is a commercially important plant genus, which has been used since ancient times, particularly for medicinal purposes. Traditionally, Paste, gum, and smoke from leaves and pods are applied for anticancer, antidiabetic, anti-inflammatory, and antimicrobial purposes. Components of Prosopis such as flavonoids, tannins, alkaloids, quinones, or phenolic compounds demonstrate potentials in various biofunctions, such as analgesic, anthelmintic, antibiotic, antiemetic, microbial antioxidant, antimalarial, antiprotozoal, antipustule, and antiulcer activities; enhancement of H+, K+, ATPases; oral disinfection; and probiotic and nutritional effects; as well as in other biopharmaceutical applications, such as binding abilities for tablet production. The compound juliflorine provides a cure in Alzheimer disease by inhibiting acetylcholine esterase at cholinergic brain synapses. Some indirect medicinal applications of Prosopis spp. are indicated, including antimosquito larvicidal activity, chemical synthesis by associated fungal or bacterial symbionts, cyanobacterial degradation products, “mesquite” honey and pollens with high antioxidant activity, etc. This review will reveal the origins, distribution, folk uses, chemical components, biological functions, and applications of different representatives of Prosopis.

  17. Banana (Musa. spp.) strain HD-1 appraisal

    International Nuclear Information System (INIS)

    Longyan, G.; Xinguo, L.; Lingxia, W.; Xuefei, J.

    2016-01-01

    Being one of the important tropical and subtropical fruit trees, banana (Musa spp.) belongs to the family Musaceae and the order Scitaminae with two genera, Musa and Ensete. In a field survey, research team has discovered a potential banana mutant strain HD-1 with a sound economic value. The results of the finding are as follows: based on Simmonds classification, the pseudostem of banana strain HD-1 is relatively short and purplish red; its upright outward petiole groove has red edges and wraps its pseudostem loosely. Its ploidy is 3, AAA type. Karyotype analysis shows that the number of chromosomes is 33, the karyotype formula is 2n=3x=33=2L + 3 M2 + 4 M1 + 2 S, HD-1 is classified as 1B type. With the help of ISSR molecular markers, we find thatbanana HD-1 has the closest relationship with Pubei and Tianbao dwarf banana; the similarity coefficient is 0.81. In an artificial simulation tests of cold, drought and salt resistance environment changes of physiological and biochemical indexes indicate that HD-1 exhibits stronger defense capability than Brazil banana. By way of inoculation with injury of root dipping method, we respectively treat two kinds of banana seedlings inoculated Banana Fusarium wilt race 4 small species. The results show that their resistance evaluation scores are 3 and 4, disease levels are susceptible and high sensitivity respectively. We conclude that HD-1 has stronger resistance ability to Fusarium wilt than Brazil banana. (author)

  18. Biopharmaceutical potentials of Prosopis spp. (Mimosaceae, Leguminosa).

    Science.gov (United States)

    Henciya, Santhaseelan; Seturaman, Prabha; James, Arthur Rathinam; Tsai, Yi-Hong; Nikam, Rahul; Wu, Yang-Chang; Dahms, Hans-Uwe; Chang, Fang Rong

    2017-01-01

    Prosopis is a commercially important plant genus, which has been used since ancient times, particularly for medicinal purposes. Traditionally, Paste, gum, and smoke from leaves and pods are applied for anticancer, antidiabetic, anti-inflammatory, and antimicrobial purposes. Components of Prosopis such as flavonoids, tannins, alkaloids, quinones, or phenolic compounds demonstrate potentials in various biofunctions, such as analgesic, anthelmintic, antibiotic, antiemetic, microbial antioxidant, antimalarial, antiprotozoal, antipustule, and antiulcer activities; enhancement of H + , K + , ATPases; oral disinfection; and probiotic and nutritional effects; as well as in other biopharmaceutical applications, such as binding abilities for tablet production. The compound juliflorine provides a cure in Alzheimer disease by inhibiting acetylcholine esterase at cholinergic brain synapses. Some indirect medicinal applications of Prosopis spp. are indicated, including antimosquito larvicidal activity, chemical synthesis by associated fungal or bacterial symbionts, cyanobacterial degradation products, "mesquite" honey and pollens with high antioxidant activity, etc. This review will reveal the origins, distribution, folk uses, chemical components, biological functions, and applications of different representatives of Prosopis. Copyright © 2016. Published by Elsevier B.V.

  19. Analysis of constituents of metal elements and amino acids in new cultivar Citrus reticulata Blanco cv. Ougan fruit from China.

    Science.gov (United States)

    Chen, Xiangtao; Yuan, Ke; Si, Jinping

    2009-01-01

    The aim of this research is to establish a new kind of simple and rapid method to determine amino acids and metal elements in Citrus reticulata Blanco cv. Ougan fruit. The high-performance liquid chromatography pre-column derivation method was used to test the contents of amino acids found in the cv. fruit. The results showed that there were 17 kinds of amino acids in the fruits of two different mature periods. They could be separated easily within 30 min. The correlation coefficients between the peak area of amino acid and the content of the amino acid were above 0.99. The sample-added recovery rate of amino acids was between 96.0% and 102.4%. Meanwhile, the microwave-aided dissolving procedure was adopted for dissolving of the cv. fruit to determine the 15 metal elements in the cv. fruit under the best conditions of flame atomic absorption spectrophotometry. The results showed that there were high contents of trace elements and amino acids in the fruit of two different ripening periods. The correlation coefficients between the peak areas of amino acids and the concentration of the elements are satisfactory. These results may provide us with the scientific evidence for further studies and the exploitation of C. reticulata Blanco cv. Ougan.

  20. THE INFLUENCE OF JOB STRESS AND JOB STATISFACTION ON EMPLOYEE’S ORGANIZATIONAL COMMITMENT AT CV. DIPO PRODUCTION

    Directory of Open Access Journals (Sweden)

    Widya Paramita

    2016-09-01

    Full Text Available The purpose of this research are: 1 To find a description of the job stress, job satisfaction and organizational commitment at CV. Dipo Production, 2 To determine whether there is an influence of job stress on employee’s organizational commitment at CV. Dipo Production, 3 To determine whether there is an influence of job statisfaction on employee’s organizational commitment at CV. Dipo Production, 4 To determine whether there is influence of job stress and job statisfaction simultaneously on employee’s organizational commitment at CV. Dipo Production. The sample of this research are all employees who work as a Tour Leader at CV. Dipo Production.Research data retrieval by survey method with questionnaires and the data processed with SPSS version 22.This research using descriptive and explanatory analysis. The Results of regression showed that job stress has a negative and significant effect on organizational commitment. While job statisfaction has a positive and significant effect on organizational commitment.

  1. Susceptibilidad de genotipos de Solanum spp. al nematodo causante del nudo radical Meloidogyne spp. (chitwood

    Directory of Open Access Journals (Sweden)

    Gelpud Chaves Cristian

    2011-03-01

    Full Text Available

    El cultivo del lulo (Solanum quitoense L. presenta una disminución en su productividad, debido al ataque de patógenos como el nematodo del nudo radical Meloidogyne  spp., en el Departamento  de Nariño (Colombia, se han reportado incidencias cercanas al 79%, y pérdidas del 50%.   En la presente investigación, se colectaron 45 genotipos de (Solanum quitoense  L. en los Departamentos  de Nariño  y Putumayo  y 4 genotipos  silvestres  (S. mammosum, S. hirtum,       S. marginatum  y S. umbellatum buscando fuentes de resistencia al nematodo. Se inocularon 9 plantas de cada genotipo de dos meses de edad con 10000 huevos de Meloidogyne spp., dejando tres testigos por cada material. Las variables evaluadas fueron: altura de planta, severidad, incidencia, peso fresco (tallo y raíz y especies prevalentes de Meloidogyne spp. Se hizo una clasificación de genotipos mediante escala de resistencia y regresión entre la severidad y las demás variables para establecer el efecto de Meloidogyne spp. sobre los genotipos de planta. Los resultados mostraron 100% de incidencia del nematodo en  todos  los  genotipos,  2.04%  genotipos  resistentes,  34.7%  moderadamente  resistentes, 42.8% moderadamente susceptibles, 18.3% susceptibles, y 2.04% altamente susceptibles. El genotipo SQbr05 resistente, no se vio afectado por la severidad, al contrario SQbc04 genotipo susceptible, mostró reducciones significativas en peso fresco de tallo y raIz, (R2 = 0.71 y 0.98,el genotipo silvestre (S. mammosum es altamente susceptible, Meloidogyne incognita presentó 55.31% de presencia. El genotipo SQbr05 es promisorio para ser evaluado en campo.

  2. A review of Sarcocystis spp. shed by opossums (Didelphis spp. in Brazil

    Directory of Open Access Journals (Sweden)

    Samantha Yuri Oshiro Branco Valadas

    2016-08-01

    Full Text Available South American opossums are the definitive hosts of Sarcocystis neurona, Sarcocystis falcatula, Sarcocystis speeri and Sarcocystis lindsayi. The sporocysts of these species of Sarcocystis are morphologically similar and methods like infectivity and pathogenicity for intermediate hosts (immunodeficient mice and psittacine birds and molecular tools are used for identification. Opossums are synanthropic wild animals, and widely distributed in Brazilian territory. Previous studies have shown high environmental contamination with S. neurona sporocysts in several Brazilian regions. This paper reviews information on Sarcocystis spp. shed by various opossum species and its occurrence in Brazil.

  3. High efficiency transformation of banana [Musa acuminata L. cv. Matti (AA)] for enhanced tolerance to salt and drought stress through overexpression of a peanut salinity-induced pathogenesis-related class 10 protein.

    Science.gov (United States)

    Rustagi, Anjana; Jain, Shalu; Kumar, Deepak; Shekhar, Shashi; Jain, Mukesh; Bhat, Vishnu; Sarin, Neera Bhalla

    2015-01-01

    Bananas and plantains (Musa spp. L.) are important subsistence crops and premium export commodity in several countries, and susceptible to a wide range of environmental and biotic stress conditions. Here, we report efficient, rapid, and reproducible Agrobacterium-mediated transformation and regeneration of an Indian niche cultivar of banana [M. acuminata cv. Matti (AA)]. Apical meristem-derived highly proliferative multiple shoot clump (MSC) explants were transformed with the Agrobacterium strain EHA105 harboring a binary vector pCAMBIA-1301 carrying hptII and uidA. Sequential agro-infiltration (10 min, 400 mmHg), infection (additional 35 min, Agrobacterium density A 600 = 0.8) and co-cultivation (18 h) regimen in 100 µM acetosyringone containing liquid medium were critical factors yielding high transformation efficiency (~81 %) corroborated by transient GUS expression assay. Stable transgenic events were recovered following two cycles of meristem initiation and selection on hygromycin containing medium. Histochemical GUS assay in several tissues of transgenic plants and molecular analyses confirmed stable integration and expression of transgene. The protocol described here allowed recovery of well-established putative transgenic plantlets in as little as 5 months. The transgenic banana plants could be readily acclimatized under greenhouse conditions, and were phenotypically similar to the wild-type untransformed control plants (WT). Transgenic plants overexpressing Salinity-Induced Pathogenesis-Related class 10 protein gene from Arachis hypogaea (AhSIPR10) in banana cv. Matti (AA) showed better photosynthetic efficiency and less membrane damage (P < 0.05) in the presence of NaCl and mannitol in comparison to WT plants suggesting the role of AhSIPR10 in better tolerance of salt stress and drought conditions.

  4. [The relevance of Candida spp. in chronic periodontal disease].

    Science.gov (United States)

    Razina, I N; Chesnokova, M G; Nedoseko, V B

    The aim of the study was to assess the correlation of Candida spp. incidence in periodontal tissues with various clinical manifestations of chronic periodontal disease (CPD). Ninety patients with CPD were included in the study in which Candida spp. was evaluated in periodontal pockets content and gingival biopsy material. In severe CPD more Candida spp. were seen in gingival biopsy than in periodontal pockets (p=0.0006). Candida spp. incidence and quantity correlated directly with the disease grade showing incidence increase from 40 to 73.3% and quantity increase from 0.8±0.18 до 3.6±0.49 lg CFU/ml in light and severe CPD, correspondingly Candida spp. had statistically significant association with cyanotic gingival color (p=0.0018), tongue plaque and swelling (р=0.0042), lip exfoliation (р=0.0030), periodontal pockets depth >5 mm (р=0.0030), oral mucosa hyperemia (р=0.0157), alveolar bone destruction >1/2 of root length (р=0.0157). These data prove the relevance of Candida spp. and mycological assessment of gingival biopsy in CPD patients.

  5. The impact of cold on photosynthesis in genotypes of Coffea spp.--photosystem sensitivity, photoprotective mechanisms and gene expression.

    Science.gov (United States)

    Batista-Santos, P; Lidon, F C; Fortunato, A; Leitão, A E; Lopes, E; Partelli, F; Ribeiro, A I; Ramalho, J C

    2011-05-15

    Environmental constraints disturb plant metabolism and are often associated with photosynthetic impairments and yield reductions. Among them, low positive temperatures are of up most importance in tropical plant species, namely in Coffea spp. in which some acclimation ability has been reported. To further explain cold tolerance, the impacts on photosynthetic functioning and the expression of photosynthetic-related genes were analyzed. The experiments were carried out along a period of slow cold imposition (to allow acclimation), after chilling (4°C) exposure and in the following rewarming period, using 1.5-year-old coffee seedlings of 5 genotypes with different cold sensitivity: Coffea canephora cv. Apoatã, Coffea arabica cv. Catuaí, Coffea dewevrei and 2 hybrids, Icatu (C. arabica×C. canephora) and Piatã (C. dewevrei×C. arabica). All genotypes suffered a significant leaf area loss only after chilling exposure, with Icatu showing the lowest impact, a first indication of a higher cold tolerance, contrasting with Apoatã and C. dewevrei. During cold exposure, net photosynthesis and Chl a fluorescence parameters were strongly affected in all genotypes, but stomatal limitations were not detected. However, the extent of mesophyll limitation, reflecting regulatory mechanisms and/or damage, was genotype dependent. Overnight retention of zeaxanthin was common to Coffea genotypes, but the accumulation of photoprotective pigments was highest in Icatu. That down-regulated photochemical events but efficiently protected the photosynthetic structures, as shown, e.g., by the lowest impacts on A(max) and PSI activity and the strongest reinforcement of PSII activity, the latter possibly reflecting the presence of a photoprotective cycle around PSII in Icatu (and Catuaí). Concomitant to these protection mechanisms, Icatu was the sole genotype to present simultaneous upregulation of caCP22, caPI and caCytf, related to, respectively, PSII, PSI and to the complex Cytb(6)/f

  6. Development of multiplex real-time PCR assay for the detection of Brucella spp., Leptospira spp. and Campylobacter foetus.

    Science.gov (United States)

    Selim, Abdelfattah M; Elhaig, Mahmoud M; Gaede, Wolfgang

    2014-12-29

    Abortion among dairy cattle is one of the major causes of economic losses in the livestock industry. This study describes a 1-step multiplex real-time polymerase chain reaction (PCR) to detect Brucella spp., Leptospira spp. and Campylobacter foetus, these are significant bacteria commonly implicated in bovine abortion. ß-actin was added to the same PCR reaction as an internal control to detect any extraction failure or PCR inhibition. The detection limit of multiplex real-time PCR using purified DNA from cultured organisms was set to 5 fg for Leptospira spp. and C. foetus and to 50 fg for Brucella spp. The multiplex real-time PCR did not produce any non-specific amplification when tested with different strains of the 3 pathogens. This multiplex real-time PCR provides a valuable tool for diagnosis, simultaneous and rapid detection for the 3 pathogens causing abortion in bovine.

  7. Development of multiplex real-time PCR assay for the detection of Brucella spp., Leptospira spp. and Campylobacter foetus

    Directory of Open Access Journals (Sweden)

    Abdelfattah M. Selim

    2014-12-01

    Full Text Available Abortion among dairy cattle is one of the major causes of economic losses in the livestock industry. This study describes a 1-step multiplex real-time polymerase chain reaction (PCR to detect Brucella spp., Leptospira spp. and Campylobacter foetus, these are significant bacteria commonly implicated in bovine abortion. ß-actin was added to the same PCR reaction as an internal control to detect any extraction failure or PCR inhibition. The detection limit of multiplex real-time PCR using purified DNA from cultured organisms was set to 5 fg for Leptospira spp. and C. foetus and to 50 fg for Brucella spp. The multiplex real-time PCR did not produce any non-specific amplification when tested with different strains of the 3 pathogens. This multiplex real-time PCR provides a valuable tool for diagnosis, simultaneous and rapid detection for the 3 pathogens causing abortion in bovine.

  8. Molecular Detection of Legionella spp. and their associations with Mycobacterium spp., Pseudomonas aeruginosa and amoeba hosts in a drinking water distribution system

    Data.gov (United States)

    U.S. Environmental Protection Agency — Quantity of Legionella spp., Mycobacterium spp., Acanthamoeba,Vermamoeba vermiformis and Pseudomonas aeruginosa were estimated using qPCR methods. This dataset is...

  9. Estudio para la mejora del trazado y seguridad vial de la carretera CV-20 entre el municipio de Montanejos y la intersección con la carretera CV-198 (Provincia de Castellón)

    OpenAIRE

    GIMENO GIL, SALVADOR

    2017-01-01

    [EN] The object of this study is the analysis of the deficiencies that are presented on the CV-20 road between Montanejos and the intersection with the CV-198, in order to propose an alternative that, in accordance with the regulations and various criteria that satisfy the comfort and expectations of the driver, solve the problems detected. In this way it will be possible to improve the safety and comfort of the road users. First, based on the data provided by the related stations and tho...

  10. Reactivation of UV- and γ-irradiated herpes virus in UV- and X-irradiated CV-1 cells

    International Nuclear Information System (INIS)

    Takimoto, K.; Niwa, O.; Sugahara, T.

    1982-01-01

    Enhanced reactivation of UV- and γ-irradiated herpes virus was investigated by the plaque assay on CV-1 monkey kidney monolayer cells irradiated with UV light or X-rays. Both UV- and X-irradiated CV-1 cells showed enhancement of survival of UV-irradiated virus, while little or no enhancement was detected for γ-irradiated virus assayed on UV- or X-irradiated cells. The enhanced reactivation of UV-irradiated virus was greater when virus infection was delayed 24 or 48 h, than for infection immediately following the irradiation of cells. Thus the UV- or X-irradiated CV-1 cells are able to enhance the repair of UV damaged herpes virus DNA, but not of γ-ray damaged ones. (author)

  11. A microsatellite library for Carica papaya L. cv. Sunrise solo Marcadores microssatélites para Carica papaya L. cv. Sunrise solo

    Directory of Open Access Journals (Sweden)

    Silvia Correa Santos

    2003-08-01

    Full Text Available In experimental areas of the Education and Researches Ilha Solteira and Jaboticabal UNESP/Campus farms were selected and tagged 20 hermaphrodite plants and 20 feminine of cultivar Sunrise Solo, Improved Sunrise Solo cv.72/12 and Baixinho of Santa Amália.The seeds origined of the selected fruits were cropped to be analysed the self-pollination efficiency and frequency of the sex in the progenies. After that, samples of the young leaf of the matrix plants were colected for the extration of the DNA. It was built five library enriched of microsatellite sequencies, using probes (TCA10, (TC13, (GATA4, (CAC10 e (TGAG8.It was possible the development of the primers only in the library that has utilized the probe (TCA10. This probe allowed the design of 32 primer pairs. From these, 31 presented pattern of unique band in agarose Metaphor and in acrilamide. For primer S36 were observed 2 bands, but with no polymorphism to differentiation in the sexual form at papaya tree culture. However, these primers can be tested, in the futures, in the investigation of the others features in segregated populations of this specie and the related species, germoplasm analysis, cultivars identification, parent evolution and molecular markers for the assisted plant breeding programs.Em áreas experimentais das Fazendas de Ensino e Pesquisa da UNESP/Campus de Ilha Solteira e Jaboticabal foram selecionadas e marcadas 20 plantas hermafroditas e 20 femininas dos cultivares Sunrise Solo, Improved Sunrise Solo cv.72/12 e Baixinho de Santa Amália. As sementes provenientes dos frutos selecionados foram plantadas para analisar-se a eficiência da autofecundação e a freqüência dos sexos nas progênies. Posteriormente, amostras de tecido foliar jovem das plantas matrizes foram coletadas para a extração de DNA. Foram construídas cinco bibliotecas enriquecidas de seqüências microsatélites, utilizando-se as sondas (TCA10, (TC13, (GATA4, (CAC10 e (TGAG8. Foi possível o

  12. Avaliação da cv. Merlot para elaboração de vinho tinto Evaluation of cv. Merlot to make red wine

    Directory of Open Access Journals (Sweden)

    Luiz Antenor Rizzon

    2003-12-01

    Full Text Available A uva Merlot é uma das responsáveis pelas características dos vinhos tintos de Saint Émillion, região de Bordeaux, França. Foi introduzida no Rio Grande do Sul através da Estação Agronômica de Porto Alegre. É, atualmente, uma das cultivares tinta de Vitis vinifera L. com maior volume de produção da Serra Gaúcha. É utilizada para a elaboração de vinho tinto para ser consumido jovem. O objetivo do presente trabalho foi avaliar o potencial enológico da cv. Merlot para a elaboração de vinho tinto fino. Para isso, realizaram-se estudos para caracterizar o cacho, o mosto e o vinho nas safras de 1987 a 1994, em Bento Gonçalves, RS. Os resultados evidenciaram que a cv. Merlot tem cacho com peso médio de 161,9g e baga esférica e pequena, pesando 1,61g. No mosto, o valor médio de açúcar é de 18,4ºBrix; o da acidez total, de 104meqL-1; e o do pH, de 3,21. O vinho se caracteriza por ter teores médios de 10,8% v/v de álcool; 70,3meqL-1 de acidez titulável; e pH de 3,49. Sob o aspecto sensorial, o vinho Merlot apresenta cor vermelho-violáceo com boa intensidade. Gustativamente distingue-se pela maciez, equilíbrio e fineza.Merlot is a traditional grape of the Saint Émillion wines, Bordeaux region - France. It was introduced into, the State of Rio Grande do Sul by the Estação Agronômica de Porto Alegre, Brazil. Today, of the red grape cultivars it shows the largest production in the Serra Gaúcha region. The purpose of this work was to evaluate the enological potential of Merlot grapes for red winemaking. To achieve this purpose, variables related to berry clusters, must and wine were measured between 1987 and 1994 vintages, from Merlot vineyards located in the municipality of Bento Gonçalves. The results showed that Merlot grapes have clusters of medium size (161.9g and small berries (1.61g. The must showed adequate sugar level (18.4ºBrix, high titratable acidity (104mqL-1 and medium pH 3.21. The wine Merlot had an

  13. Avaliação da cv. Tannat para elaboração de vinho tinto Evaluation of cv. Tannat to elaborate red wine

    Directory of Open Access Journals (Sweden)

    Luiz Antenor Rizzon

    2004-06-01

    Full Text Available A uva Tannat, originária do Sudoeste da França, foi introduzida no Rio Grande do Sul, pela Estação Experimental de Caxias do Sul. É uma cultivar difundida na Serra Gaúcha para elaboração de vinho tinto, utilizado para corte e para a produção de vinho varietal. O objetivo do presente trabalho foi avaliar o potencial enológico da cv. Tannat para a elaboração de vinho tinto fino nessa região vitícola. Para isso, realizaram-se estudos para caracterizar o cacho, o mosto e o vinho nas safras de 1987 a 1994, a partir de um vinhedo localizado em Bento Gonçalves, RS. Os resultados evidenciaram que a cv. Tannat tem cacho com peso médio de 216,1g e baga esférica pequena (1,54g. O mosto apresenta teor elevado de acidez titulável (155meq/L-1. O vinho se caracteriza por teores elevados em: polifenóis totais (I 280= 43,0, taninos (1,9g/L, antocianinas (539mg/L, potássio (1.856mg/L-1 e soma dos álcoois superiores (512mg/L-1. As características sensoriais do vinho Tannat na Serra Gaúcha evidenciam uma cor vermelha-violácea intensa, tânicos, nervosos, relativamente duros e aromas de frutas vermelhas. É recomendável para corte com outros vinhos tintos finos para intensificar sua cor e estrutura e também para a produção de vinho varietal podendo melhorar com o amadurecimento em barricas de carvalho.The Tannat grape is native to the Southwest of France, and was introduced into the State of Rio Grande do Sul by the Caxias do Sul Experimental Station. It is a cultivar diffused in the Serra Gaucha region with the purpose of elaborating varietal red wine for blending. The purpose of this work was to evaluate the enological potential of the Tannat grape for red wine in this region. To achieve this objective, variables related to the berries, clusters, must, and wine were evaluated from the 1987 to 1994 vintages, in a vineyard established in Bento Gonçalves, RS, Brazil. The results showed that Tannat grapes have large clusters (216.1g and

  14. Estiolamento na micropropagação do Abacaxizeiro cv. Pérola Etiolated in micropropagation of cv. Pérola Pineapple plant

    Directory of Open Access Journals (Sweden)

    Maria Aparecida Moreira

    2003-10-01

    Full Text Available Objetivou-se estudar o efeito do estiolamento na micropropagação de abacaxizeiro cv. Pérola e posterior recuperação dos brotos estiolados, realizando-se dois experimentos. No primeiro, os caules utilizados como explantes foram obtidos de brotos pré-estabelecidos in vitro, dos quais foram retiradas as folhas. O estiolamento foi induzido colocando-se os explantes em tubos de ensaio no escuro por 20, 40 e 80 dias, contendo os seguintes meios de cultura: 1 MS suplementado com 0,1 mg.L-1 de ANA e 0,5 mg.L-1 de BAP, 2 MS suplementado com 1,8 mg.L-1 de ANA e 2 mg.L-1 de BAP e 3 MS sem reguladores de crescimento. Para número de brotos, o melhor meio foi o MS + 1,8 mg.L-1 de ANA e 2 mg.L-1 de BAP, obtendo-se média de 10,26 brotações aos 40 dias no escuro. Para comprimento de brotos estiolados, o meio MS sem reguladores de crescimento foi significativamente melhor quando os explantes foram mantidos por 80 dias no escuro, apresentando comprimento médio de 10,86 cm. No segundo experimento, os brotos estiolados, com e sem o ápice, foram colocados horizontalmente em placas contendo meios de cultura com idêntica formulação aos descritos anteriormente. Para número total de brotos, brotações estioladas sem ápice em meio MS suplementado com 1,8mg.L-1 de ANA e 2 mg.L-1 de BAP promoveram o melhor resultado, correspondendo a 10,61 brotações por explante.It was aimed to produce micropropagated plantlets of pineapple cv. Pérola by using the etiolated technique and subsequent recovery of etiolated shoots. Two experiments were carried out. In the first, the stalks used as explant were obtained from in vitro shoots established without leaves. The etiolation was induced by putting the explants in test tubes in the darkness for 20, 40 and 80 days with the media: 1 MS supplemented with ANA 0.1mg.L-1 and BAP 0.5mg.L-1, 2 MS supplemented with ANA 1.8mg.L-1 and BAP 2mg.L-1 and 3 MS without growth regulators. The best results for shoot number were obtained

  15. Energy saving in Bellota Mexico S.A. de C.V.; Ahorro de energia en Bellota Mexico S.A de C.V.

    Energy Technology Data Exchange (ETDEWEB)

    Salazar Lopez, Jose Albeiro [Bellota Mexico S.A. de C.V. (Mexico)

    2003-07-01

    The present article intends to make a general description of the different activities that were developed in Bellota Mexico S.A. de C.V. during year 2001 and which lead to obtaining the national prize of energy saving granted by the Comision Federal de Electricidad (CFE) and the Fideicomiso para el Ahorro de Energia Electrica (FIDE). In the executed actions stands out an entirely coordinated program that includes: lighting systems, motors, refrigeration systems, transformers, special projects (settling tanks), capacitors, compressed air pipeline, induction furnaces, machinery in general, maximum demand control, preventive maintenance, measuring systems, operational and organizational systems. In all these subjects a fast description is made of the performed actions and the obtained results. It is mentioned in addition the importance of making a power diagnosis and the use of historical data for the planning of the strategies to follow. Also reference is made of the use of appropriate technology that considers the use of high-energy efficiency equipment, such as motors, lamps, compressors, etc. Finally an evaluation is made of the obtained results where the improvement of the electrical parameters stands out such as: reduction of the power index in 35.06%, reduction of the maximum demand in 11.94%, increase of the power factor from 93.53% in 98.21%. The obtained results demonstrate that investing in energy saving it is a good business. [Spanish] El presente trabajo tiene por objeto hacer una descripcion general de las diferentes actividades que se desarrollaron en Bellota Mexico S.A. de C.V. durante el ano 2001 y que condujeron a la obtencion del premio nacional de ahorro de energia otorgado por la Comision Federal de Electricidad (CFE) y el Fideicomiso para el Ahorro de Energia Electrica (FIDE). En las acciones ejecutadas se destaca todo un programa coordinado que abarca: sistemas de iluminacion, motores, sistemas de refrigeracion, transformadores, proyectos

  16. Induced mutations in cucumber for resistance to nematodes (Meloidogyne spp.)

    International Nuclear Information System (INIS)

    Udalov, V.B.; Prikhod'ko, V.F.

    1989-01-01

    Full text: Seeds were treated with EI and selection was carried out over 5 years. In the susceptible cv. ''M15'' treated with 0.05% EI for 21 h 7.6% of plants were found resistant, 72% only slightly susceptible. Mutants were used in crosses and hybrids showed 57-89% resistance over 6 generations. (author)

  17. Assessment of Duplex PCR for the simultaneous diagnose of Mycobacterium spp. and Brucella spp. in cattle

    Directory of Open Access Journals (Sweden)

    Ariel Escobar

    2013-03-01

    Full Text Available Tuberculosis and brucellosis remain important causes of morbidity and mortality in many countries, for the detection of both diseases requires efficient and sensitive tool for effectuate the diagnosis. This study was aimed to evaluate and compare the duplex PCR versus the nested PCR, for detection of Brucella spp. (BR and Mycobacterium spp. (TB. A total of 100 samples of tissues from tracheo-bronchial lymph nodes, bovine lung and bacterial isolate as positive controls were used. Were evaluated ten combinations of primers which were designed to flank the segment of the 16S rRNA sequence (RB and antigen gen MPB70 (TB, the best result for the Duplex PCR was obtained with the primers Bru-2F/Bru-2R for BR and Tub-1F/Tub-N-R for TB. The amplification of the products was 225 and 230-bp respectively. In order to compare the results of the proposed technique, all samples were initially analyzed and compared between PCR and nested PCR (Kappa, k = 0.85 and the concordance between Duplex PCR and nested PCR (k = 0.88 for the two bacteria was very good.

  18. Existence of Cryptosporidium spp. oocysts and Giardia spp. cysts in well water in Nineveh governorate

    Directory of Open Access Journals (Sweden)

    R. G. Mohammad

    2011-01-01

    Full Text Available The study included examination of 110 water samples from well distributed in Mosul city and few towns and villages around it from May 2009 to March 2010 for detection of Cryptosporidium spp oocysts and Giardia spp cysts in well water. The results revealed that the prevalence of Cryptosporidium oocysts was 16.36% while the prevalence of Giardia cysts was 12.72%. The percentages of prevalence with Cryptosporidium and Giardia were in high rate in Bartilla and some villages around it 20% for Cryptosporidium and 17.14% for Giardia, the low rates were in Mosul city 10% for both protozoa. The highest prevalence rate of Cryptosporidium was in March 38.46% and the lowest was in November and July 0%. The highest prevalence rate of Giardia was in October 23.53% and the lowest rate in July 0%. This first study shows the presence of Cryptosporidium oocysts and Giardia cysts in well water (Ground water in Nineveh governorate.

  19. ACIDENTES POR SERPENTE (BOTHROPS SPP. E CROTALLUS SPP. EM CRIANÇAS: RELATO DE DOIS CASOS

    Directory of Open Access Journals (Sweden)

    Beatriz Ferreira Martins

    2012-01-01

    Full Text Available Los accidentes provocados por serpientes poseen importancia sanitaria debido a la frecuencia y gravedad. La estandarización de la atención es imprescindible para auxiliar a los equipos de salud en el diagnóstico precoz y en el tratamiento específico a las víctimas. Pero, la evolución clínica del caso presenta particularidades, principalmente en accidentes infantiles y en ancianos. El objetivo fue describir y analizar dos casos clínicos de accidentes infantiles con serpientes de los géneros Bothrops spp. y Crotallus spp., notificados a un centro de información y asistencia toxicológica del Noroeste del Paraná/Brasil. Se encontraron aspectos positivos en la atención, como precocidad del acceso a la unidad de salud, identificación de síntomas y seroterapia precoz, y no ocurrencia de reacciones adversas a la seroterapia antivenenosa, y como aspectos negativos, la ausencia de la solicitud de exámenes bioquímicos preconizados y alta hospitalaria precoz pos seroterapia, considerando la posibilidad de reacciones adversas.

  20. Infestation of Pseudopiazurus papayanus (Marshall) (Coleoptera: Curculionidae) on Carica spp. and Vasconcella spp. genotypes

    International Nuclear Information System (INIS)

    Fancelli, Marilene; Sanches, Nilton F.; Dantas, Jorge L.L.; Caldas, Ranulfo C.; Morales, Cinara F.G.

    2008-01-01

    The papaya borer weevil, Pseudopiazurus papayanus (Marshall), is generally considered a secondary pest, but it has been reported in high infestations in Northeast Brazil. This work aimed at evaluating the occurrence of P. papayanus and reporting its infestation level in papaya genotypes kept at the germplasm bank of EMBRAPA Cassava and Tropical Fruits (Cruz das Almas, Bahia, Brazil). The number of larvae, pupae and adults found in each plant of 65 Carica spp. genotypes and of three Vasconcella spp. genotypes was registered in three to five plants of each genotype, by cutting the exsudating trunks lengthwise. Papaya borer weevil was found in C. papaya and V. cauliflora but not in those of V. quercifolia. Among the evaluated genotypes, 52.4% of those belonging to the Solo group were infested, against 25.0% of the Formosa group. Larval infestation was the best criterion for sorting out genotypes concerning this insect infestation. This is also the first occurrence of the papaya borer weevil . (author)

  1. Prevalence of antibiotic resistant coliform bacteria, Enterococcus spp. and Staphylococcus spp. in wastewater sewerage biofilm.

    Science.gov (United States)

    Lépesová, Kristína; Kraková, Lucia; Pangallo, Domenico; Medveďová, Alžbeta; Olejníková, Petra; Mackuľak, Tomáš; Tichý, Jozef; Grabic, Roman; Birošová, Lucia

    2018-03-28

    Urban wastewater contains different micropollutants and high number of different microorganisms. Some bacteria in wastewater can attach to the surfaces and form biofilm, which gives bacteria advantage in fight against environmental stress. This work is focused on bacterial community analysis in biofilms isolated from influent and effluent sewerage of wastewater treatment plant in Bratislava. Biofilm microbiota detection was performed by culture-independent and culture-dependent approaches. Composition of bacterial strains was detected by denaturing gradient gel electrophoresis fingerprinting coupled with the construction of 16S rRNA clone libraries. The biofilm collected at the inlet point was characterized primarily by the presence of Pseudomonas sp., Acinetobacter sp. and Janthinobacterium sp. clones, while in the biofilm isolated at outflow of wastewater treatment plant members of Pseudomonas genus were largely detected. Beside this analysis prevalence of antibiotics and resistant coliforms, Enterococcus spp. and Staphylococcus spp. in sewerage was studied. In influent wastewater were dominant antibiotics like azithromycin, clarithromycin and ciprofloxacin. Removal efficiency of these antibiotics notably azithromycin and clarithromycin were 30% in most cases. The highest number of resistant bacteria with predominance of coliforms was detected in sample of effluent biofilm. Multidrug resistant strains in effluent biofilm showed very good ability to form biofilm. Copyright © 2018. Published by Elsevier Ltd.

  2. Plasmodium spp. and Haemoproteus spp. infection in birds of the Brazilian Atlantic Forest detected by microscopy and polymerase chain reaction

    Directory of Open Access Journals (Sweden)

    Raquel Tostes

    2015-01-01

    Full Text Available In recent years haemosporidian infection by protozoa of the genus Plasmodium and Haemoproteus, has been considered one of the most important factors related to the extinction and/or population decline of several species of birds worldwide. In Brazil, despite the large avian biodiversity, few studies have been designed to detect this infection, especially among wild birds in captivity. Thus, the objective of this study was to analyze the prevalence of Plasmodium spp. and Haemoproteus spp. infection in wild birds in captivity in the Atlantic Forest of southeastern Brazil using microscopy and the polymerase chain reaction. Blood samples of 119 different species of birds kept in captivity at IBAMA during the period of July 2011 to July 2012 were collected. The parasite density was determined based only on readings of blood smears by light microscopy. The mean prevalence of Plasmodium spp. and Haemoproteus spp. infection obtained through the microscopic examination of blood smears and PCR were similar (83.19% and 81.3%, respectively, with Caracara plancus and Saltator similis being the most parasitized. The mean parasitemia determined by the microscopic counting of evolutionary forms of Plasmodium spp. and Haemoproteus spp. was 1.51%. The results obtained from this study reinforce the importance of the handling of captive birds, especially when they will be reintroduced into the wild.

  3. Transcriptomic signatures of ash (Fraxinus spp. phloem.

    Directory of Open Access Journals (Sweden)

    Xiaodong Bai

    2011-01-01

    Full Text Available Ash (Fraxinus spp. is a dominant tree species throughout urban and forested landscapes of North America (NA. The rapid invasion of NA by emerald ash borer (Agrilus planipennis, a wood-boring beetle endemic to Eastern Asia, has resulted in the death of millions of ash trees and threatens billions more. Larvae feed primarily on phloem tissue, which girdles and kills the tree. While NA ash species including black (F. nigra, green (F. pennsylvannica and white (F. americana are highly susceptible, the Asian species Manchurian ash (F. mandshurica is resistant to A. planipennis perhaps due to their co-evolutionary history. Little is known about the molecular genetics of ash. Hence, we undertook a functional genomics approach to identify the repertoire of genes expressed in ash phloem.Using 454 pyrosequencing we obtained 58,673 high quality ash sequences from pooled phloem samples of green, white, black, blue and Manchurian ash. Intriguingly, 45% of the deduced proteins were not significantly similar to any sequences in the GenBank non-redundant database. KEGG analysis of the ash sequences revealed a high occurrence of defense related genes. Expression analysis of early regulators potentially involved in plant defense (i.e. transcription factors, calcium dependent protein kinases and a lipoxygenase 3 revealed higher mRNA levels in resistant ash compared to susceptible ash species. Lastly, we predicted a total of 1,272 single nucleotide polymorphisms and 980 microsatellite loci, among which seven microsatellite loci showed polymorphism between different ash species.The current transcriptomic data provide an invaluable resource for understanding the genetic make-up of ash phloem, the target tissue of A. planipennis. These data along with future functional studies could lead to the identification/characterization of defense genes involved in resistance of ash to A. planipennis, and in future ash breeding programs for marker development.

  4. Paraneoplastic Choreoathetosis in a Patient with Small Cell Lung Carcinoma and Anti-CRMP5/CV2: A Case Report

    DEFF Research Database (Denmark)

    Lassen, Lisbeth Landschoff; Somnier, Finn; Aydin, Dogu

    2016-01-01

    Introduction: The occurrence of more or less monosymptomatic paraneoplastic choreoathetosis associated with anti-CRMP5/CV2 antibodies is rare. Typically, such autoantibodies are associated with a more classical syndrome - paraneoplastic encephalomyelitis. Frequently, small cell lung carcinoma (SCLC...... 14 months after the onset of the symptoms. Conclusion: We report paraneoplastic choreoathetosis associated with anti-CRMP5/CV2 antibodies. Such published case histories are rare. Although expected, we did not find any reduced signal intensity at the basal ganglia on the T1-weighted or increased...

  5. Sonication inhibited browning but decreased polyphenols contents and antioxidant activity of fresh apple (malus pumila mill, cv. Red Fuji) juice.

    Science.gov (United States)

    Sun, Yujing; Zhong, Liezhou; Cao, Lianfei; Lin, Wenwen; Ye, Xingqian

    2015-12-01

    Enzyme browning is the main challenge in the preparation of fresh apple juice. The influence of sonication on browning, as well as polyphenols and antioxidant activity of fresh apple juice was investigated. It was found that ultrasound can inhibit the browning of fresh apple (Malus pumila Mill, cv. Red Fuji) juice, but decreased the contents of total phenolic content (TPC), total flavonoid content (TFC) and chlorogenic acid and reduced the antioxidant activity. On the whole, ultrasound technology cannot be used to the antibrowning of fresh apple (Malus pumila Mill, cv. Red Fuji) juice.

  6. [FISH analysis of chromosomes of sweet potato(Ipomoea batatas cv.Xushu No.18)].

    Science.gov (United States)

    Tang, Jia-Li; Qi, Da-Shi; Zhang, Yu; Liu, Hui-Juan; Sun, Jian-Ying; Cao, Qing-He; Ma, Dai-Fu; Li, Zong-Yun

    2010-02-01

    In order to understand the chromosome structure of sweet potato (Ipomoea batatas cv. Xushu 18), molecular cytogenetic analyses were carried out on I. batatas. by using 45S rDNA fluorescence in situ hybridization (45S rDNA-FISH), self genomic in situ hybridization (self-GISH), and silver staining techniques. Twelve, sixteen, and eighteen regions were silver stained in the interphase nucleus of I. batatas. The results of FISH analysis demonstrated 16 or 18 signals with different intensity on chromosomes of I. batatas. Self-GISH analysis showed that the intensive signals on I. batatas mitotic chromosomes were distributed along the chromosomes. However, the signals located in centromeric, subcentromeric, and telomeric regions were stronger and denser than those in other regions.

  7. Analysis of morphological traits of bird's foot trefoil plants cv. Bokor transformed with Agrobacterium rhizogenes

    Directory of Open Access Journals (Sweden)

    Nikolić Radomirka

    2005-01-01

    Full Text Available An efficient method for genetic transformation and shoot regeneration was achieved in bird's foot trefoil cv. Bokor using A. rhizogens. The transformed shoots were regenerated on hairy root segments in high frequency. After rooting and acclimation, transformed To plants were grown in experimental field. Analysis of morphological traits and chemical content in ten unintentionally chosen To bird's foot trefoil plants (genotypes no. 2 and no. 5 was performed. They were compared to those of control non-transformed plants. The traits as a number of stems per plant, length of internodes in longest stem, number of flowers per plant and plan high were very significant differed than the same traits in control plants, while there were no significant differences in the leaf area. No signs of the rol genes genotype and "T" phenotype were present. The transformed plants had significantly higher content of cellulose, while the protein and nitrogen contents of are in the range of control plants.

  8. T4 RIA precision profiles automatically computed using a small programmable pocket calculator HP-41 CV

    International Nuclear Information System (INIS)

    Ditu, M.; Waud, J.M.; Hamilton, R.G.; Wagner, H.N.

    1984-01-01

    Precision profiles as useful tool for assurance of assay quality in 10 independent T4 RIAs have been automatically obtained using a small programmable pocket calculator HP-41 CV. For each T4 assay batch, the within-assay coefficient of variation varied from 7 to 11% in the hormone concentration range of 2 to 20μg/dl. The difference in coefficient of variation for all the 10 successive assay batches of T4 never exceeded 1% in the same hormone concentrations regions. All the above findings demonstrate that the precision profile can be used as a powerful tool for assessing the assay quality and consistency of overall random error between successive assay batches [fr

  9. Proliferation Potential of 18-Month-Old Callus of Ananas comosus L. cv. Moris

    Directory of Open Access Journals (Sweden)

    A.E. De Silva

    2006-01-01

    Full Text Available Differential effect of plant growth regulators and additives in proliferation of 18-month-old calli of Ananas comosus L. cv. Moris were assessed in vitro. The proliferation of callus relied on the growth regulators and additives. Of the different auxins supplemented in the Murashige and Skoog (MS media, 32.22 μM α-naphthaleneacetic acid (NAA gave the highest mean fresh weight of callus (46.817 g. Medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D was inferior to NAA, while b-naphthoxy acetic acid (BNOA and p-chlorophenoxy acetic acid (4-CPA were not effective in proliferating 18-months old callus. Addition of casein hydrolysate and coconut water to NAA supplemented medium showed better proliferation and production of callus. However, in terms of callus production, NAA at 32.22 μM was economically better.

  10. Odour-active compounds in guava (Psidium guajava L. cv. Red Suprema).

    Science.gov (United States)

    Pino, Jorge A; Bent, Leandra

    2013-09-01

    Solid phase microextraction and simultaneous distillation-extraction combined with GC-FID, GC/MS, aroma extract dilution analysis and odour activity values were used to analyse volatile compounds from guava (Psidium guajava L. cv. Red Suprema) and to estimate the most odour-active compounds. The analysis led to the detection of 141 compounds, 121 of which were positively identified. The composition of guava fruit volatiles included 43 esters, 37 terpenes, 18 aldehydes, 16 alcohols, ten acids, six ketones, four furans and seven miscellaneous compounds. Seventeen odorants were considered as odour-active compounds, with (E)-β-ionone, ethyl hexanoate, ethyl butanoate, hexanal, (Z)-3-hexenal, hexyl acetate, (E)-2-hexenal and limonene contributing most to the typical guava aroma of this cultivar. © 2013 Society of Chemical Industry.

  11. Purification, characterization, thermal, and high-pressure inactivation of pectin methylesterase from bananas (cv Cavendish).

    Science.gov (United States)

    Ly Nguyen, B; Van Loey, A; Fachin, D; Verlent, I; Indrawati; Hendrickx, M; Hendrickx, I M

    2002-06-20

    Pectin methylesterase (PME) was extracted from bananas (cv Cavendish) and purified by affinity chromatography on a CNBr-Sepharose-PME inhibitor (PMEI) column. A single protein and PME activity peak was obtained. For banana PME, a biochemical characterization in terms of molar mass (MM), pI, and kinetic parameters was performed. In a second step, the thermal and high-pressure stability of the enzyme was studied. Isothermal inactivation of purified banana PME could be described by a first-order kinetic model in a temperature range of 65 degrees to 72.5 degrees C, whereas its isobaric-isothermal inactivation followed a fractional-conversion model. Banana PME was found to be more thermally stable compared with PMEs extracted from orange, tomato, and apple. Copyright 2002 Wiley Periodicals, Inc.

  12. Study of the TAF 2012 DriveLine (CV-CD

    Directory of Open Access Journals (Sweden)

    Alexandru POZDÎRCĂ

    2016-06-01

    Full Text Available TAF is the acronym for Articulated Forestry Tractor – a heavy vehicle uses in a logging operation for pulling cut trees out of a forest, in a process called “skidding”. TAF use a cardan shafts transmission from the gear box (CV to distribution box (CD. The distribution box split the movement thru axels or winch. The study applied the cardan theory on a specific case and based on the numeric results a few design modifications are proposed for reducing the angular velocity pulse on the CD input shaft. Theoretical results may have a significant importance in practice, impacting the reduction of transmission noise and vibrations - already claimed in the case of the distribution box for the current constructive solution. An original AutoLISP program are made in order to analyze and optimize the transmission, with graphical results.

  13. Physiological responses of PEA (Pisum sativum cv. meteor) to irrigation salinity

    International Nuclear Information System (INIS)

    Shahid, M.A.; Pervez, M.A.; Balal, R.M.; Azhar, N.; Shahzad, J.; Ubaidullah

    2008-01-01

    The effects of irrigation water or soil salinity on physiological aspects of pea (Pisum sativum cv.Meteor) were contrived. Ten weeks old pea plants were treated with NaCl at 0, 40, 90 and 140 mM in nutrient solution Plants were grown in controlled environment and harvested at each 3 days interval for decisiveness 0 physiological parameters. Photosynthetic rate, relative water content, stomatal conductance and chlorophyll contents reduced by increasing the NaCI concentration while CO/sub 2/ concentration and free proline content intensified. By experiment it was adumbrated that high salinity level along with prolonged accentuate duration is more drastic to pea plants physiology. Results also exhibited that pea plants could indulge 40 and 90 mM NaCl but are sensitive to 140 mM. (author)

  14. Micropropagation from cultured nodal explants of rose (Rosa hybrida L. cv. ‘Perfume Delight’

    Directory of Open Access Journals (Sweden)

    Kamnoon Kanchanapoom

    2010-01-01

    Full Text Available A method for the micropropagation of rose (Rosa hybrida L. cv. ‘Perfume Delight’ was developed. First to fifth nodal explants from young healthy shoots were excised and cultured on basal medium of Murashige and Skoog (1962, MS containing several concentrations of BA and NAA. Multiple shoot formation of up to 3 shoots was obtained on MS medium supplemented with 3 mg/l BA and 0.003 mg/l NAA. Shoot readily rooted on ¼MS medium devoid of growth regulators.Rooted plantlets were hardened and established in pots at 100% survival. In vitro flowering was observed on rose plantscultured on MS medium containing 3 mg/l BA and 0.003 mg/l NAA.

  15. Changes in endogenous growth inhibitors in onion bulbs (Allium cepa L. cv. Sochaczewska during storage

    Directory of Open Access Journals (Sweden)

    Elżbieta Kielak

    2013-12-01

    Full Text Available Changes in inhibitor activity in the onion bulbs (Allium cepa L. cv. Sochaczewska during storage were investigated. Onions were dried under an umbrella roof until October 15th or November 15th and thereafter stored in a cold-room at 0-1°C until May 15th. The activity of inhibitors fluctuated markedly during the storage period. At least two peaks and two decreases of inhibitor activity were observed. The weather conditions seemed to strongly influence the level and the date of appearance of inhibitors in onions. Higher inhibitor activity is usually connected with better storage and less sprouting of onions during storage. Prolonged drying under an umbrella roof enhanced onion quality after storage only in these cases when it actually improved the drying of onions.

  16. Development of seedlings of watermelon cv. Crimson Sweet irrigated with biosaline water

    Directory of Open Access Journals (Sweden)

    José E. S. B. da Silva

    2015-09-01

    Full Text Available ABSTRACTThe limited access and the scarcity of good quality water for agriculture are some of the major problems faced in agricultural areas, particularly in arid and semiarid regions. The aim of this study was to evaluate the quality of watermelon seedlings (cv. Crimson Sweet, irrigated with different concentrations of biosaline water of fish culture. The experimental design was completely randomized with five treatments, corresponding to biosaline water at different concentrations (0, 33, 50, 67 and 100%, and four replicates of 108 seedlings. Watermelon seeds were sown in plastic trays filled with commercial substrate and irrigated with different solutions of biosaline water. Seedlings were harvested for biometric analysis at 14, 21 and 28 days after sowing. The use of biosaline water did not affect emergence and establishment of seedlings until 14 days after sowing, the period recommended for transplantation. However, the use of biosaline water affected the development of seedlings with longer exposure time.

  17. Effect of Gamma Irradiation to Yellow Flesh Watermelon cv. ''Huay Sai Thong''

    International Nuclear Information System (INIS)

    Puripunyavanich, Vichai

    2003-06-01

    Yellow flesh watermelon seeds cv. Huay Sai Thong was irradiated with gamma rays. The experiment has been shown LD 50 dose at 420 Gy and GR 50 dose at 480 Gy approximately. Seeds of watermelon irradiated at 200 and 400 Gy were sown to find out mutation characteristics. The growth rate of M 1 plants was lower than the normal one. In M 2 plants, some irradiated lines showed non-dark green stripe on surface of fruit. Genetic testing was done according to skin color on watermelon fruit. It was found that the normal stripe characteristic was dominant and non-stripe characteristic was recessive. The Short length vine characteristic was selected till M 7 generation. The two lines of non-stripe watermelon and two lines of normal-stripe watermelon were selected for short length vine characteristic

  18. Humudifucol and Bioactive Prenylated Polyphenols from Hops (Humulus lupulus cv. "Cascade").

    Science.gov (United States)

    Forino, Martino; Pace, Simona; Chianese, Giuseppina; Santagostini, Laura; Werner, Markus; Weinigel, Christina; Rummler, Silke; Fico, Gelsomina; Werz, Oliver; Taglialatela-Scafati, Orazio

    2016-03-25

    Humulus lupulus (hop plant) has long been used in traditional medicine as a sedative and antimicrobial agent. More recently, attention has been devoted to the phytoestrogenic activity of the plant extracts as well as to the anti-inflammatory and chemopreventive properties of the prenylated chalcones present. In this study, an Italian sample of H. lupulus cv. "Cascade" has been investigated and three new compounds [4-hydroxycolupulone (6), humudifucol (7) and cascadone (8)] have been purified and identified by means of NMR spectroscopy along with four known metabolites. Notably, humudifucol (7) is the first prenylated dimeric phlorotannin discovered in nature. Because structurally related phloroglucinols from natural sources were found previously to inhibit microsomal prostaglandin E2 synthase (mPGES)-1 and 5-lipoxygenase (5-LO), the isolated compounds were evaluated for their bioactivity against these pro-inflammatory target proteins. The prenylated chalcone xanthohumol inhibited both enzymes at low μM concentrations.

  19. Compositional investigation of phenolic polymers isolated from Vitis vinifera L. Cv. Pinot Noir during fermentation.

    Science.gov (United States)

    Aron, Patricia M; Kennedy, James A

    2007-07-11

    Phenolic polymer material extracted during commercial red wine fermentations (Vitis vinifera L. cv. Pinot noir) was isolated and analyzed to characterize its chemical composition. Phenolic polymer isolates were prepared from samples taken throughout fermentation and isolated by adsorption chromatography. Isolates were subjected to phloroglucinolysis to analyze the proanthocyanidin amount as well as the subunit composition. Results of phloroglucinolysis revealed that the proanthocyanidin content of individual phenolic polymer isolates varied from 27 to 54%. Subsequent analyses were done in an attempt to quantify materials other than known proanthocyanidin subunits. Results of all experiments indicate that up to 82% of the phenolic polymer isolates could be accounted for by mass. While this figure accounts for a significant portion of the polymeric phenolic material, further investigation will be needed to qualify the remaining 18%.

  20. Increasing sustainability through the use of organic matters/manures in banana production. cv. harichal

    International Nuclear Information System (INIS)

    Miano, T.F.; Baloch, M.A.; Baloch, A.F.; Miano, T.F.

    2005-01-01

    A banana experiment was carried out with cv. Harichal under the ecological conditions of Tando Jam to study the effect of organic manures/matter on the growth and bunch weight (yield) of banana. The treatments applied were ; FYM, Dry leaves, Stalk of the banana bunch and control with constant doses of NPK (136g + 57g + 148g per plant). Minimum days (490.33) from planting to harvest were observed under the treatment of FYM followed by stalk of the bunch and dried leaves. The highest single fruit weight (107 g), fruit length( 18.30 cm) bunch weight (25.46 kg) and calculated yield per hectare (33.80 tons) were observed under FYM with NPK fertilizer followed by stalk of the bunch and dried leaves. (author)

  1. Use of nootkatone as a senescence indicator for Rouge La Toma Cv. grapefruit (Citrus paradisi Macf.).

    Science.gov (United States)

    Biolatto, Andrea; Sancho, Ana M; Cantet, Rodolfo J C; Güemes, Daniel R; Pensel, Norma A

    2002-08-14

    The objective of this research was to study the usefulness of nootkatone as a senescence indicator for Rouge La Toma cv. grapefruit (Citrus paradisi Macf.), simulating different treatments that included the normal postharvest handling of citrus fruits: temperature conditioning, cold storage, shipment periods to overseas markets such as Japan and the U.S., marketing conditions, and storage at nonchilling temperature (control treatments). The highest nootkatone levels, determined by GLC-MS analyses, were detected in fruits subjected to control treatments. No significant differences were observed in nootkatone levels between treatments either with or without temperature conditioning prior to the start of the cold storage. Levels of nootkatone increased throughout time for all assayed treatments. The linear regressions of nootkatone levels showed correlation coefficients of 0.80 and 0.83 with storage time (29 and 42 days, respectively). Therefore, nootkatone appears to be a good indicator of senescence for Rouge La Toma grapefruit.

  2. Induction of in vivo mutation in chrysanthemum (Dendranthema grandiflora Tzvelev) cv. Pink Repin breeding

    International Nuclear Information System (INIS)

    Neto, Augusto Tulmann; Latado, Rodrigo Rocha

    1997-01-01

    Mutation induction was used with the objective of obtaining mutants for flower colour of chrysanthemum, cv. Repin (pink colour). Rooted cuttings were irradiated with 20 Gy of gamma rays and before the selection the cutting back method was used to advance the generations. The frequency of colour mutants observed was 5.8%. Among the mutants obtainedthe white and dark-pink-coloured ones were evaluated in yield trial and post-harvest. The results indicated that these mutants mantained the same agronomical characteristics showed by the control, with the exception of plant height in the white mutant that was shorter. Due to commercial interest of the producers, these mutants were multiplied and released as new cultivars. The white flower colour mutant was named Cristiane and the dark-pink, Ingrid. This was the first example of cultivars from an ornamental plant released by mutation breeding in Brazil. (author)

  3. Sensory analysis and volatile compounds of olive oil (cv. Cobrancosa) from different irrigation regimes

    Energy Technology Data Exchange (ETDEWEB)

    Fernandes-Silva, A. A.; Falco, V.; Correia, C. M.; Villalobos, F. J.

    2013-05-01

    The aim of this study was to assess the effect of different irrigation strategies on the sensory quality of virgin olive oil VOO) from the cv. cobrancosa- integrated into a protected denomination of origin of Azeite de Tras-os-Montes in the Northeast of Portugal. Three irrigation treatments were applied: (T2)-full irrigation, which received a seasonal water equivalent of 100% of the estimated crop evapotranspiration (ET{sub c}), (T1)-continuous deficit irrigation (30% ETc) and (T0)- rainfed treatment. Data were collected from two consecutive crop years (2005-2006). Olive oil samples were analyzed for volatiles by GC-MS and the results compared with sensory evaluation data. Total volatile compounds tended to decrease with the amount of water applied. The characteristics pungent and bitter were more pronounced in olive oils from T0 and T1, which had higher polyphenolic concentrations, with a strong positive relationship with this variable and the bitter attribute. The Principal Components Analysis clearly separates the three olive oils from 2005, the driest year, and aggregates into a single group the three samples from 2006, suggesting no effect of irrigation on volatile compounds in years with a rainy spring and a marked effect in years with severe drought, suggesting that the effect of the trees’ water status on these variables occurs throughout the crop season and not just during the oil accumulation phase. In general, olive oil from the cv. Cobrançosa is more bitter than pungent and has a typical nutty sensory attribute shown by a strong positive relationship between benzaldehyde and the sensory notes of almonds and nuts. (Author) 34 refs.

  4. PARIWISATA KREATIF DAN KEGIATAN EKSTRAKURIKULER BERBASIS BAMBU DALAM PENGEMBANGAN MODEL BISNIS CV SURATIN BAMBOO

    Directory of Open Access Journals (Sweden)

    Dewa Ayu Tenara Kardinia Cidhy

    2016-09-01

    Full Text Available The objective of this study was to design a prototype of a future business model which would be adopted to achieve balance between the aspects of new business opportunities and competitive advantages of CV Suratin Bamboo. The analytical method utilized in the study was the business model canvas approach (BMC supported by the blue ocean strategy and SWOT.  The results revealed that through this business model development, bamboo workshop is expected to provide information regarding bamboo derived products, raise awareness of preserving the indigenous bamboo culture, increase sense of belonging to domestic products, as well as become the primary support of innovation and ideas in constructing Indonesia as a nation. Meanwhile, for businesses actors, besides increasing revenue, conducting bamboo workshops is expected to increase the company growths for the long term. The opportunity to maneuver in the development of bamboo-based business model demonstrates that bamboo business with high quality products has both tangible and intangible potentials to be developed further.Keywords: bamboo, blue ocean strategy, business model canvas, creative tourism, extracurricularABSTRAKTujuan penelitian ini adalah merancang prototype model bisnis masa depan yang akan digunakan untuk menemukan keseimbangan antara aspek peluang bisnis yang baru dan aspek keuntungan kompetitif CV Suratin Bamboo. Metode analisis yang digunakan adalah dengan pendekatan kanvas model bisnis (BMC dilengkapi dengan blue ocean strategy serta SWOT. Hasil penelitian menunjukkan melalui pengembangan model bisnis ini, pelatihan bambu diharapkan mampu memberikan informasi tentang produk turunan bambu, meningkatkan kesadaran melestarikan kearifan lokal budaya bambu, meningkatkan rasa cinta produk dalam negeri, serta menjadi pendorong lahirnya inovasi dan gagasan yang membangun bangsa Indonesia. Sementara bagi pelaku usaha, selain peningkatan revenue, pelatihan bambu diharapkan mampu meningkatkan

  5. Rooting and acclimatization of the Japanese plum tree, cv. América

    Directory of Open Access Journals (Sweden)

    Juliana de Magalhães Bandeira

    2012-06-01

    Full Text Available Rooting and acclimatization are limiting steps in plant micropropagation, especially in woody plant species. This study aimed to evaluate the IAA and IBA effect on the in vitro rooting and acclimatization of micropropagated shoots of Japanese plum (Prunus salicina Lindl. cv. América. Shoots from 3 to 4 cm long were inoculated in MS medium with half salt and vitamin concentrations (MS/2 added with IAA and IBA (0, 0.25, 0.5, 0.75 and 1 mg L-1. After a 20-day period in in vitro cultivation, the shoots were evaluated, and then transferred to a greenhouse, and evaluated after 30 days. At the end of the in vitro cultivation period, no significant interactions were observed for number of roots per shoot and rooting percentage, but a significant effect was recorded for auxin type only, for which shoots grown in media added with IBA showed high values - 0.87 and 41.95%, respectively. A linear increase response from 1.45 to 5.75 cm was verified for root length of shoots cultivated in IBA medium; however, no significant effect was observed, and a 0.86 cm average root length per shoot grown in medium added with IAA was found. After 30 days of acclimatization period, the largest survival percentage was obtained from shoots cultivated in medium with 1 mg L-1 of IBA and IAA (88% and 92%, respectively. Although, IBA provided the highest in vitro rooting, most of the surviving shoots were those originated in IAA-added medium, probably because IBA promoted longer fibrous roots, less appropriate for transplant and soil fixation, as they are easily damaged. It was concluded that in vitro rooting with the addition of the highest IAA concentration (1 mg L-1 provided the greatest plant survival during the acclimatization period of the Japanese plum cv. América.

  6. [Phenolic compounds in leaves insertions of Mentha × villosa Huds. cv. Snežná].

    Science.gov (United States)

    Tekeľová, Daniela; Bittner Fialová, Silvia; Tóth, Jaroslav; Czigle, Szilvia

    Lamiaceae plants mostly accumulate active ingredients in their leaves. The subfamily Nepetoideae, including the genus Mentha L., is characterized by the presence of essential oil and antioxidant phenolics, chiefly hydroxycinnamic acids with predominance of rosmarinic acid, and flavonoids. Mentha × piperita and M. spicata are the most broadly used mints in both medicine and industry, while M. x villosa is less known in our country. Herbal drugs in the form of leaves are usually analysed unpartitioned, while single leaves insertions have only been studied occasionally. Therefore, the aim of this work was the quantification of the active compounds content in the leaves pairs of Mentha × villosa Huds. cv. Snežná, using pharmacopoeial methods: total hydroxycinnamic derivatives expressed as rosmarinic acid (THD) and luteolin-type flavonoids. THD content ranged from 6.7% to 9.4% in the leaves pairs water extracts, and from 6.6% to 14.0% in methanol extracts. Flavonoids contents, expressed as luteolin-7-O-glucoside, ranged from 4.0% to 8.8% in water extracts, and from 4.0% to 10.5% in methanol extracts. Antioxidant activity (DPPH) expressed as SC50 ranged from 10.2 to 16.9 μg.ml-1 (drug dry weight) in water extracts, and from 10.7 to 21.6 μg.ml-1 in methanol extracts. The highest content of phenolic compounds as well as the highest antioxidant activity were found to be in the top sheet, while the lowest content of phenolic compounds and lowest antioxidant activity were detected in the leaves of the middle stem part.Key words: Mentha × villosa Huds cv. Snežná hydroxycinnamic derivatives rosmarinic acid luteolin-7-O-glucoside DPPH.

  7. The Rosa chinensis cv. Viridiflora phyllody phenotype is associated with misexpression of flower organ identity genes

    Directory of Open Access Journals (Sweden)

    Huijun Yan

    2016-07-01

    Full Text Available Phyllody is a flower abnormality in which leaf-like structures replace flower organs in all whorls. Here we investigated the origin and the molecular mechanism of phyllody phenotype in Rosa chinensis cv. Viridiflora, an ancient naturally occurring Chinese mutant cultivar. Reciprocal grafting experiments and microscopy analyses, demonstrated that the phyllody phenotype in Viridiflora is not associated with phytoplasmas infection. Transcriptome comparisons by the mean of RNA-Seq identified 672 up-regulated and 666 down-regulated genes in Viridiflora compared to its closely related genotype R. chinensis cv. Old Blush. A fraction of these genes are putative homologues of genes known to be involved in flower initiation and development. We show that in flower whorl 2 of Viridiflora, a down-regulation of the floral organ identity genes RcPISTILLATA (RcPI, RcAPETALA3 (RcAP3 and RcSEPALLATA3 (RcSEP3, together with an up-regulation of the putative homologue of the gene SUPPRESSOR of OVEREXPRESSION of CONSTANS1 (RcSOC1 are likely at the origin of the loss of petal identity and leaf-like structures formation. In whorl 3 of Viridiflora, ectopic expression of RcAPETALA2 (RcAP2 along with the down regulation of RcPI, RcAP3 and RcSEP3 is associated with loss of stamens identity and leaf-like structures formation. In whorl 4, the ectopic expression of RcAP2 associated with a down-regulation of RcSEP3 and of the C-class gene RcAGAMOUS correlate with loss of pistil identity. The latter also suggested the antagonist effect between the A and C class genes in the rose. Together, these data suggest that modified expression of the ABCE flower organ identity genes is associated with the phyllody phenotype in the rose Viridiflora and that these genes are important for normal flower organs development.

  8. Presence of Borrelia spp. DNA in ticks, but absence of Borrelia spp. and of Leptospira spp. DNA in blood of fever patients in Madagascar.

    Science.gov (United States)

    Hagen, Ralf Matthias; Frickmann, Hagen; Ehlers, Julian; Krüger, Andreas; Margos, Gabriele; Hizo-Teufel, Cecilia; Fingerle, Volker; Rakotozandrindrainy, Raphael; Kalckreuth, Vera von; Im, Justin; Pak, Gi Deok; Jeon, Hyon Jin; Rakotondrainiarivelo, Jean Philibert; Heriniaina, Jean Noël; Razafindrabe, Tsiry; Konings, Frank; May, Jürgen; Hogan, Benedikt; Ganzhorn, Jörg; Panzner, Ursula; Schwarz, Norbert Georg; Dekker, Denise; Marks, Florian; Poppert, Sven

    2018-01-01

    The occurrence of tick-borne relapsing fever and leptospirosis in humans in Madagascar remains unclear despite the presence of their potential vectors and reservoir hosts. We screened 255 Amblyomma variegatum ticks and 148 Rhipicephalus microplus ticks from Zebu cattle in Madagascar for Borrelia-specific DNA. Borrelia spp. DNA was detected in 21 Amblyomma variegatum ticks and 2 Rhipicephalus microplus ticks. One Borrelia found in one Rhipicephalus microplus showed close relationship to Borrelia theileri based on genetic distance and phylogenetic analyses on 16S rRNA and flaB sequences. The borreliae from Amblyomma variegatum could not be identified due to very low quantities of present DNA reflected by high cycle threshold values in real-time-PCR. It is uncertain whether these low numbers of Borrelia spp. are sufficient for transmission of infection from ticks to humans. In order to determine whether spirochaete infections are relevant in humans, blood samples of 1009 patients from the highlands of Madagascar with fever of unknown origin were screened for Borrelia spp. - and in addition for Leptospira spp. - by real-time PCR. No target DNA was detected, indicating a limited relevance of these pathogens for humans in the highlands of Madagascar. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Molecular Detection of Legionella spp. and their associations with Mycobacterium spp., Pseudomonas aeruginosa and amoeba hosts in a drinking water distribution system.

    Science.gov (United States)

    Lu, J; Struewing, I; Vereen, E; Kirby, A E; Levy, K; Moe, C; Ashbolt, N

    2016-02-01

    This study investigated waterborne opportunistic pathogens (OPs) including potential hosts, and evaluated the use of Legionella spp. for indicating microbial water quality for OPs within a full-scale operating drinking water distribution system (DWDS). To investigate the occurrence of specific microbial pathogens within a major city DWDS we examined large volume (90 l drinking water) ultrafiltration (UF) concentrates collected from six sites between February, 2012 and June, 2013. The detection frequency and concentration estimates by qPCR were: Legionella spp. (57%/85 cell equivalent, CE l(-1) ), Mycobacterium spp. (88%/324 CE l(-1) ), Pseudomonas aeruginosa (24%/2 CE l(-1) ), Vermamoeba vermiformis (24%/2 CE l(-1) ) and Acanthamoeba spp. (42%/5 cyst equivalent, CE l(-1) ). There was no detection of the following microorganisms: human faecal indicator Bacteroides (HF183), Salmonella enterica, Campylobacter spp., Escherichia coli O157:H7, Giardia intestinalis, Cryptosporidium spp. or Naegleria fowleri. There were significant correlations between the qPCR signals of Legionella spp. and Mycobacterium spp., and their potential hosts V. vermiformis and Acanthamoeba spp. Sequencing of Legionella spp. demonstrated limited diversity, with most sequences coming from two dominant groups, of which the larger dominant group was an unidentified species. Other known species including Legionella pneumophila were detected, but at low frequency. The densities of Legionella spp. and Mycobacterium spp. were generally higher (17 and 324 folds, respectively) for distal sites relative to the entry point to the DWDS. Legionella spp. occurred, had significant growth and were strongly associated with free-living amoebae (FLA) and Mycobacterium spp., suggesting that Legionella spp. could provide a useful DWDS monitoring role to indicate potential conditions for non-faecal OPs. The results provide insight into microbial pathogen detection that may aid in the monitoring of microbial water

  10. Significance and Roles of Proteus spp. Bacteria in Natural Environments.

    Science.gov (United States)

    Drzewiecka, Dominika

    2016-11-01

    Proteus spp. bacteria were first described in 1885 by Gustav Hauser, who had revealed their feature of intensive swarming growth. Currently, the genus is divided into Proteus mirabilis, Proteus vulgaris, Proteus penneri, Proteus hauseri, and three unnamed genomospecies 4, 5, and 6 and consists of 80 O-antigenic serogroups. The bacteria are known to be human opportunistic pathogens, isolated from urine, wounds, and other clinical sources. It is postulated that intestines are a reservoir of these proteolytic organisms. Many wild and domestic animals may be hosts of Proteus spp. bacteria, which are commonly known to play a role of parasites or commensals. However, interesting examples of their symbiotic relationships with higher organisms have also been described. Proteus spp. bacteria present in soil or water habitats are often regarded as indicators of fecal pollution, posing a threat of poisoning when the contaminated water or seafood is consumed. The health risk may also be connected with drug-resistant strains sourcing from intestines. Positive aspects of the bacteria presence in water and soil are connected with exceptional features displayed by autochthonic Proteus spp. strains detected in these environments. These rods acquire various metabolic abilities allowing their adaptation to different environmental conditions, such as high concentrations of heavy metals or toxic substances, which may be exploited as sources of energy and nutrition by the bacteria. The Proteus spp. abilities to tolerate or utilize polluting compounds as well as promote plant growth provide a possibility of employing these microorganisms in bioremediation and environmental protection.

  11. Protein Chips for Detection of Salmonella spp. from Enrichment Culture

    Directory of Open Access Journals (Sweden)

    Palmiro Poltronieri

    2016-04-01

    Full Text Available Food pathogens are the cause of foodborne epidemics, therefore there is a need to detect the pathogens in food productions rapidly. A pre-enrichment culture followed by selective agar plating are standard detection methods. Molecular methods such as qPCR have provided a first rapid protocol for detection of pathogens within 24 h of enrichment culture. Biosensors also may provide a rapid tool to individuate a source of Salmonella contamination at early times of pre-enrichment culture. Forty mL of Salmonella spp. enrichment culture were processed by immunoseparation using the Pathatrix, as in AFNOR validated qPCR protocols. The Salmonella biosensor combined with immunoseparation showed a limit of detection of 100 bacteria/40 mL, with a 400 fold increase to previous results. qPCR analysis requires processing of bead-bound bacteria with lysis buffer and DNA clean up, with a limit of detection of 2 cfu/50 μL. Finally, a protein chip was developed and tested in screening and identification of 5 common pathogen species, Salmonella spp., E. coli, S. aureus, Campylobacter spp. and Listeria spp. The protein chip, with high specificity in species identification, is proposed to be integrated into a Lab-on-Chip system, for rapid and reproducible screening of Salmonella spp. and other pathogen species contaminating food productions.

  12. Opuntia spp.: Characterization and Benefits in Chronic Diseases

    Directory of Open Access Journals (Sweden)

    María del Socorro Santos Díaz

    2017-01-01

    Full Text Available Opuntia species have been used for centuries as food resources and in traditional folk medicine for their nutritional properties and their benefit in chronic diseases, particularly diabetes, obesity, cardiovascular diseases, and cancer. These plants are largely distributed in America, Africa, and the Mediterranean basin. Opuntia spp. have great economic potential because they grow in arid and desert areas, and O. ficus-indica, the domesticated O. species, is used as a nutritional and pharmaceutical agent in various dietary and value-added products. Though differences in the phytochemical composition exist between wild and domesticated (O. ficus-indica Opuntia spp., all Opuntia vegetatives (pear, roots, cladodes, seeds, and juice exhibit beneficial properties mainly resulting from their high content in antioxidants (flavonoids, ascorbate, pigments (carotenoids, betalains, and phenolic acids. Other phytochemical components (biopeptides, soluble fibers have been characterized and contribute to the medicinal properties of Opuntia spp. The biological properties of Opuntia spp. have been investigated on cellular and animal models and in clinical trials in humans, allowing characterization and clarification of the protective effect of Opuntia-enriched diets in chronic diseases. This review is an update on the phytochemical composition and biological properties of Opuntia spp. and their potential interest in medicine.

  13. Comprehensive and comparative lipidome analysis of Vitis vinifera L. cv. Pinot Noir and Japanese indigenous V. vinifera L. cv. Koshu grape berries

    Science.gov (United States)

    Arita, Kayo; Honma, Taro

    2017-01-01

    Vitis vinifera cv. Koshu is an indigenous grape cultivar that has been cultivated for more than a thousand years in Japan and one of the most important cultivars in white winemaking. To improve Koshu wine quality, it is necessary to identify the metabolites in Koshu berry. We conducted a comprehensive and comparative lipidome analysis of Koshu and Pinot Noir berries cultivated in the same location in Japan using GC-MS/MS for fatty acids and LC-MS for glycerolipids and glycerophospholipids. Koshu skins and juices contained 22 and 19 fatty acids, respectively, whereas 23 and 20 fatty acids were detected in Pinot Noir skins and juices. C22:6n3 and C24:0 contents in Koshu skins were two and three times higher than those in Pinot Noir skins. C24:0 content in Koshu juices was also higher than that in Pinot Noir juices. Forty-nine lipid components (six digalactosyldiacylglycerols, one monogalactosyldiacylglycerol, 10 phosphatidylcholines, 12 phosphatidylethanolamines, and 20 triglycerides) were detected in Pinot Noir and Koshu skins. Strong peaks were observed for MGDG 36:6, DGDG 36:6, PC 34:2, PC 36:5, TG 54:6, TG 54:7, and TG 54:8 in Koshu skins. The contents of 36 of the 49 lipid components were significantly higher in Pinot Noir skins than Koshu skins. Pinot Noir skins contained more lipids whose alkyl chains have more than 18 carbons than Koshu skins. Further analysis of both lipid profiles revealed that the number of double bonds in a fatty acid molecule in Pinot Noir skins and juices was significantly larger than that in Koshu skins and juices. A strong relationship exists between the heat requirement of grapevine cultivars and the level of fatty acid desaturation. C18-fatty acids were the major components in Koshu and Pinot Noir berries. The expression levels of C18-fatty acid desaturases regulated the accumulation of C18-unsaturated fatty acids in berry skins. The loss of C18:3 in Koshu berries at the end of ripening was observed. Koshu might effectively convert

  14. AVALIAÇÃO DA CV. CABERNET FRANC PARA ELABORAÇÃO DE VINHO TINTO EVALUATION OF CV. CABERNET FRANC TO ELABORATE RED WINE

    Directory of Open Access Journals (Sweden)

    Luiz Antenor RIZZON

    2001-08-01

    Full Text Available A uva Cabernet Franc, originária da região de Bordeaux, França, foi introduzida no Rio Grande do Sul através da Estação Agronômica de Porto Alegre. É utilizada para a elaboração de vinho tinto para ser consumido jovem, embora apresente aptidão para envelhecer. Face a sua importância, conduziu-se este trabalho com o objetivo de determinar as características agronômicas e enológicas da uva Cabernet Franc para elaboração de vinho tinto. Para isso, realizaram-se estudos para caracterizar o cacho, o mosto e o vinho tinto nas safras de 1987 a 1994. Os resultados obtidos evidenciaram que a cv. Cabernet Franc tem cacho médio, formado por bagas pequenas. O mosto possui teores de açúcar e de acidez adequados para vinificação. O vinho apresenta elevado teor de K e de álcoois superiores. No aspecto sensorial, apresenta cor vermelho-rubi, com reflexos violáceos quando jovem, e de intensidade variável em função das safras vitícolas. No olfato, apresenta aroma com notas vegetais e frutadas, que lembram pimentão e frutas vermelhas, respectivamente. Gustativamente, o vinho apresenta boa estrutura, equilíbrio e personalidade marcante.Cabernet Franc grape is originated from Bordeaux, France. It was introduced in the Serra Gaúcha region in the south of Brazil, by the Estação Agronômica the Porto Alegre, RS. It is used to elaborate young red wine, but it can be aged too. Due to the importance of Cabernet Franc, this work had the objective to determine its agronomic and winemaking characteristics. To achieve this purpose, variables related to the berries, clusters, must, and wine were performed from 1987 to 1994. The results showed that Cabernet Franc grapes have clusters of medium size and small berries. The must has adequate sugar and titratable acidity levels for vinification. The Cabernet Franc wine has a high concentration of K and higher alcohols. The sensory evaluation showed that it has a ruby color with violet reflexes when

  15. Comprehensive and comparative lipidome analysis of Vitis vinifera L. cv. Pinot Noir and Japanese indigenous V. vinifera L. cv. Koshu grape berries.

    Science.gov (United States)

    Arita, Kayo; Honma, Taro; Suzuki, Shunji

    2017-01-01

    Vitis vinifera cv. Koshu is an indigenous grape cultivar that has been cultivated for more than a thousand years in Japan and one of the most important cultivars in white winemaking. To improve Koshu wine quality, it is necessary to identify the metabolites in Koshu berry. We conducted a comprehensive and comparative lipidome analysis of Koshu and Pinot Noir berries cultivated in the same location in Japan using GC-MS/MS for fatty acids and LC-MS for glycerolipids and glycerophospholipids. Koshu skins and juices contained 22 and 19 fatty acids, respectively, whereas 23 and 20 fatty acids were detected in Pinot Noir skins and juices. C22:6n3 and C24:0 contents in Koshu skins were two and three times higher than those in Pinot Noir skins. C24:0 content in Koshu juices was also higher than that in Pinot Noir juices. Forty-nine lipid components (six digalactosyldiacylglycerols, one monogalactosyldiacylglycerol, 10 phosphatidylcholines, 12 phosphatidylethanolamines, and 20 triglycerides) were detected in Pinot Noir and Koshu skins. Strong peaks were observed for MGDG 36:6, DGDG 36:6, PC 34:2, PC 36:5, TG 54:6, TG 54:7, and TG 54:8 in Koshu skins. The contents of 36 of the 49 lipid components were significantly higher in Pinot Noir skins than Koshu skins. Pinot Noir skins contained more lipids whose alkyl chains have more than 18 carbons than Koshu skins. Further analysis of both lipid profiles revealed that the number of double bonds in a fatty acid molecule in Pinot Noir skins and juices was significantly larger than that in Koshu skins and juices. A strong relationship exists between the heat requirement of grapevine cultivars and the level of fatty acid desaturation. C18-fatty acids were the major components in Koshu and Pinot Noir berries. The expression levels of C18-fatty acid desaturases regulated the accumulation of C18-unsaturated fatty acids in berry skins. The loss of C18:3 in Koshu berries at the end of ripening was observed. Koshu might effectively convert

  16. Safety evaluation of a new anxiolytic product containing botanicals Souroubea spp. and Platanus spp. in dogs.

    Science.gov (United States)

    Masic, Aleksandar; Liu, Rui; Simkus, Kristen; Wilson, Jeff; Baker, John; Sanchez, Pablo; Saleem, Ammar; Harris, Cory C; Durst, Tony; Arnason, John T

    2018-01-01

    Separation anxiety and noise aversion are common behavioral problems in dogs. They elicit fear responses such as cowering, seeking out the owner, and attempting to escape. This can result in property damage, injury to the dog, and disruption of the owner-pet bond, possibly leading to pet abandonment or euthanasia. A novel botanical anxiolytic product was evaluated for safety in dogs as the target animal species. Its intended use is for the treatment and prevention of anxiety and noise aversion in dogs. It contains a defined mixture of Souroubea spp. vine and Platanus spp. bark, delivering the active principle, betulinic acid, at a recommended dose of 1 mg/kg body weight (BW). In the current target animal safety study, 16 healthy male beagle dogs were administered either a placebo or the newly formulated botanical tablets at 0.5×, 2.5×, or 5× the recommended dose (1 mg/kg BW) over 28 d. The dogs were monitored for occurrence of any systemic or local adverse events. In the investigation presented here, there were no clinically significant adverse effects following treatment, as determined by clinical observations, physical examinations, BW, hematology, clinical biochemistry, and urinalysis. Pharmacokinetic analysis demonstrated that the concentration of betulinic acid in serum was below 0.020 μg/mL in treated animals. Under the conditions of these studies, the formulated blend of S. sympetala and P. occidentalis, when administered up to 5× the intended dose for 28 consecutive d, showed no adverse effects on the health of dogs.

  17. Screening of Lactobacillus spp. and Pediococcus spp. for glycosidase activities that are important in oenology.

    Science.gov (United States)

    Grimaldi, A; Bartowsky, E; Jiranek, V

    2005-01-01

    To assess glycosidase activities from a range of Lactobacillus and Pediococcus species and characterize these activities under conditions pertinent to the wine industry. Lactic acid bacteria were cultured in MRS broth supplemented with apple juice before being harvested, washed and assayed for glycosidase activity using p-nitrophenol-linked substrates. All strains exhibited a detectable capacity for the hydrolysis of the beta- and alpha-d-glucopyranosides. The magnitude of these activities and their response to the physico-chemical parameters investigated varied in a strain-dependent manner. The use of an assay buffer with a pH below 4 generally resulted in a reduced hydrolysis of both substrates while temperature optima ranged between 35 and 45 degrees C. The effect of the inclusion of ethanol in the assay buffer (up to 12%, v/v) ranged from near complete inhibition to increases in activity approaching 80%. With the clear exception of a single strain, glucose and fructose (0.1-20 g l(-1)) acted as inhibitors. An assessment of glycosidase activity during simultaneous exposure to glucose and ethanol at a pH of 3.5 suggested that ethanol decreased loss of activity under these wine-like conditions. Lactobacillus spp. and Pediococcus spp. possess varying degrees of beta- and alpha-d-glucopyranosidase activities, which in turn are influenced differently by exposure to ethanol and/or sugars, temperature and pH. Several strains appeared suited for further evaluation under winemaking conditions. This work highlights the fact that strains of Lactobacillus and Pediococcus have the potential to influence the glycoside composition of wine. Tailoring of wine may therefore be possible through selective application of strains or enzymatic extracts thereof.

  18. Herbal products containing Hibiscus sabdariffa L., Crataegus spp., and Panax spp.: Labeling and safety concerns.

    Science.gov (United States)

    Nunes, Maria Antónia; Rodrigues, Francisca; Alves, Rita C; Oliveira, Maria Beatriz P P

    2017-10-01

    Herbs have been used from ancient times for infusion preparation based on their potential health effects. In particular, the consumption of Hibiscus sabdariffa L., Crataegus spp. and Panax spp. has been largely associated to cardiovascular benefits. In this work, the label information of 52 herbal products for infusion preparation containing the referred herbs was analyzed and discussed, taking into consideration the European Union regulation for herbal products, which intends to protect public health and harmonize the legal framework in Member States. Details about the cardiovascular-related statements and warning notifications about consumption were considered. Also, regulatory issues and possible herb-drug interactions were explored and discussed. A total of 14 of the 52 herbal products selected presented health claims/statements on the label. Hibiscus was present in the majority of the products and, in some cases, it was mentioned only in the ingredients list and not on the product front-of-pack. Despite the promising outcomes of these plants to modulate cardiovascular risk markers, consumers with some sort of cardiovascular dysfunction and/or under medication treatments should be aware to carefully analyze the labels and consult additional information related to these herbal products. Manufacturers have also a huge responsibility to inform consumers by presenting awareness statements. Lastly, health professionals must advise and alert their patients about possible interactions that could occur between the concomitant consumption of drugs and herbs. Overall, there is still a real need of additional studies and clinical trials to better understand herbs effects and establish a science-based guidance to assess their safety. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Mineral fertilizers improve the sensory quality of East African Highland bananas (Musa AAA-EA, cv. Kisansa)

    NARCIS (Netherlands)

    Taulya, G.; Asten, van P.J.A.; Nowakunda, K.; Kaddu-Mukasa, P.

    2010-01-01

    Some farmers in Uganda believe that fertilizers negatively affect the sensory attributes of cooking type bananas. This belief may hamper the adoption of fertilizers. To verify the validity of this belief, bunches (Musa AAA-EA, cv. ‘Kisansa’) from fertilized (i.e. N-P-K-Mg-Zn-S-B-Mo) and

  20. Boron, cobalt and molybdenum in the knottiness and production on pea (Pisum sativum L.) cv Bolero culture

    International Nuclear Information System (INIS)

    Oliveira Junior, A.; Teixeira, N.T.; Maluf, C.E.; Campos, M.F. de

    1989-01-01

    The test carried out to study the effect of Boron, Cobalt and Molybdenum on the knottiness and production of the pea (Pisum sativum L.)cv Bolero culture, in controlled conditions. The results showed that Boron didn't affect the knottiness and it increased the production, while, Cobalt and Molybdenum promoted significant increase in the knottiness and productivity. (author) [pt

  1. Inherited transgene expression of the uidA and bar genes in Lilium longiflorum cv. Nellie White

    Science.gov (United States)

    The expression of two transgenes, bar and uidA, was studied in Lilium longiflorum cv. Nellie White plants. ‘Nellie White’ had been transformed using the gene gun to bombard with pDM327 that contains the bar-uidA fusion gene under control of the CaMV 35S promoter. PCR analysis confirmed that eight ...

  2. Dehydropyrrolizidine alkaloids, including monoesters with an unusual esterifying acid, from cultivated Crotalaria juncea (Sunn Hemp cv. 'Tropic Sun')

    Science.gov (United States)

    Cultivation of Crotalaria juncea L. (Sunn Hemp cv. ‘Tropic Sun’) is recommended as a green manure crop in a rotation cycle to improve soil condition, help control erosion, suppress weeds, and reduce soil nematodes. Because C. juncea belongs to a genus that is known for the production of toxic dehydr...

  3. Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening

    Science.gov (United States)

    Fruit ripening is a physiological and biochemical process genetically programmed to regulate fruit quality parameters like firmness, flavor, odor and color, as well as production of ethylene in climacteric fruit. In this study, a transcriptomic analysis of mango (Mangifera indica L.) mesocarp cv. "K...

  4. A peroxisomally localized acyl-activating enzyme is required for volatile benzenoid formation in a Petuniaxhybrida cv. 'Mitchell Diploid' flower.

    NARCIS (Netherlands)

    Colquhoun, T.A.; Marciniak, D.M.; Wedde, A.E.; Kim, J.Y.; Schwieterman, M.L.; Levin, L.A.; Van Moerkercke, A.; Schuurink, R.C.; Clark, D.G.

    2012-01-01

    Floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis is a complex and coordinate cellular process executed by petal limb cells of a Petuniaxhybrida cv. 'Mitchell Diploid' (MD) plant. In MD flowers, the majority of benzenoid volatile compounds are derived from a core phenylpropanoid pathway

  5. Automated and connected vehicle (AV/CV) test bed to improve transit, bicycle, and pedestrian safety : concept of operations plan.

    Science.gov (United States)

    2017-02-01

    This document presents the Concept of Operations (ConOps) Plan for the Automated and Connected Vehicle (AV/CV) Test Bed to Improve Transit, Bicycle, and Pedestrian Safety. As illustrated in Figure 1, the plan presents the overarching vision and goals...

  6. The effect of vowel height on Voice Onset Time in stop consonants in CV sequences in spontaneous Danish

    DEFF Research Database (Denmark)

    Mortensen, Johannes; Tøndering, John

    2013-01-01

    Voice onset time has been reported to vary with the height of vowels following the stop consonant. This paper investigates the effects of vowel height on VOT in Danish CV sequences with stop consonants in Danish spontaneous speech. A significant effect of vowel height on VOT was found...

  7. Environmental Factors Correlated with the Metabolite Profile of Vitis vinifera cv. Pinot Noir Berry Skins along a European Latitudinal Gradient

    Czech Academy of Sciences Publication Activity Database

    Del-Castillo-Alonso, M. Á.; Castagna, A.; Csepregi, K.; Hideg, É.; Jakab, G.; Jansen, M. A. K.; Jug, T.; Llorens, L.; Mátai, A.; Martínez-Lüscher, J.; Monforte, L.; Neugart, S.; Olejníčková, Julie; Ranieri, A.; Schödl-Hummel, K.; Schreiner, M.; Soriano, G.; Teszlák, P.; Tittmann, S.; Urban, Otmar; Verdaguer, D.; Zipoli, G.; Martínez-Abaigar, J.; Núñez-Olivera, E.

    2016-01-01

    Roč. 64, č. 46 (2016), s. 8722-8734 ISSN 0021-8561 Institutional support: RVO:67179843 Keywords : berry skins * Europe * hydroxylation ratios * latitudinal gradient * phenolic composition * solar radiation * ultraviolet radiation * Vitis vinifera cv. Pinot Noir Subject RIV: EH - Ecology, Behaviour Impact factor: 3.154, year: 2016

  8. Water Deficit Effect on Ratio of Seed to Berry Fresh Weight and Berry Weight Uniformity in Winegrape cv. Merlot

    Science.gov (United States)

    Field-grown grapevines cv. Merlot (Vitis vinifera L.) were differentially irrigated in a randomized block design during two growing seasons to maintain a high or low level of vine water stress between fruit set and harvest. Detached berries from clusters harvested at maturity were individually weig...

  9. Sistemas e condições de colheita e armazenamento na qualidade de morangos cv. Camarosa Systems and conditions of harvest and storage in strawberries cv's Camarosa quality

    Directory of Open Access Journals (Sweden)

    Marcelo Barbosa Malgarim

    2006-08-01

    Full Text Available O objetivo deste trabalho foi avaliar a qualidade de morangos 'Camarosa' submetidos a diferentes sistemas de colheita, luz UV-C, atmosfera modificada e armazenamento por diferentes períodos, visando ao aumento da vida útil e à redução de perdas após a colheita. A colheita foi realizada em dois sistemas: no sistema um, o produtor realizou a colheita sem luvas, em caixas de madeira e sem utilizar o pré-resfriamento das frutas; no sistema dois, a colheita foi realizada por pessoal treinado, com utilização de luvas desinfetadas, em caixas plásticas lavadas e desinfetadas, foi empregado o pré-resfriamento a -3ºC durante 1 hora, até a temperatura da polpa das frutas atingir 4ºC. Os tratamentos foram: T1- controle (frutas sem luz UV-C e sem filme de polietileno; T2- frutas submetidas à luz UV-C durante 6 minutos; T3- frutas acondicionadas em filme de polietileno; T4- frutas submetidas à luz UV-C e acondicionadas em filme de polietileno. O armazenamento foi realizado à temperatura de 0±0,5ºC e UR de 90-95% por períodos de 3; 6 e 9 dias, seguidos de simulação da comercialização por 3 dias, à temperatura de 8±0,5ºC e UR de 75-80%. Na colheita e após o armazenamento, avaliaram-se: perda de massa; cor; firmeza de polpa; sólidos solúveis (SS; acidez total titulável (ATT; relação SS/ATT; ácido ascórbico, e incidência de podridões. A utilização de pré-resfriamento reduziu a perda de massa e a porcentagem de podridões. A perda de massa diminuiu nas frutas tratadas com filme. A luz UV-C reduziu a porcentagem de podridões. Morangos 'Camarosa' colhidos cuidadosamente, submetidos a pré-resfriamento, com utilização de luz UV-C e filme de polietileno, mantêm a qualidade durante nove dias de armazenamento a 0ºC e 3 dias de comercialização a 8ºC.The objective of this work was to evaluate the cv. Camarosa's quality of strawberries submitted to different harvest systems, UV-C light, exposure modified atmosphere and

  10. Produção integrada e convencional de pêssegos cv. Marli Integrated and convetional production of peach cv. Marli

    Directory of Open Access Journals (Sweden)

    José Luis da Silva Nunes

    2004-12-01

    Full Text Available O cultivo de pessegueiros é uma atividade de grande importância econômica no Sul do Brasil, onde se destaca o Estado do Rio Grande do Sul como maior produtor brasileiro. Um dos aspectos mais importantes na produção de alimentos da atualidade é a redução no uso de agroquímicos, com menor contaminação do ambiente e riscos reduzidos de resíduos. Este trabalho visou a comparar os sistemas de Produção Convencional (PC e Integrada (PI de pêssegos e foi realizado no ano de 2001, no município de São Jerônimo - RS, latitude 30°05'52" S, longitude 51°39'08" W e altitude de 46 metros. Áreas de um pomar comercial da cv. Marli foram avaliadas em relação às principais práticas de manejo da planta e do solo, controle fitossanitário, aspectos econômicos, bem como à qualidade da fruta. Na área conduzida sob PI, foram utilizadas as práticas de manejo preconizadas pelas Normas de Produção Integrada de Pêssegos (NPIP e, na área conduzida no sistema de PC, as plantas foram manejadas de acordo com as práticas comumente utilizadas pelo produtor. A produção de pêssegos, em ambos os sistemas, não foi afetada. Na área de PI, houve menor número de pêssegos por planta; entretanto, as frutas apresentaram maior peso médio. A maioria dos pêssegos da PI foram classificados como CAT I (diâmetro superior a 57 mm. As frutas produzidas na PC são, na maioria, de CAT II (de 48 a 57 mm. A qualidade pós-colheita não apresentou diferenças em relação à acidez, firmeza e cor. Com base nestes resultados, podemos concluir que é possível produzir pêssegos de qualidade com produtividade no sistema de PI.The peach-trees cultivation is a very economic important activity in South of Brazil, where highlights the State of Rio Grande do Sul as the largest Brazilian producer. One of the most important aspects in actuality is the food prodution with reduction of agrochemicals components, reducing the risks of environment contamination. This work

  11. Rapid detection of listeria spp. using an internalin A aptasensor based on carbon-metal nanohybrid structures

    Science.gov (United States)

    Vanegas, D. C.; Rong, Yue; Schwalb, N.; Hills, K. D.; Gomes, C.; McLamore, E. S.

    2015-05-01

    Foodborne outbreaks caused by Listeria monocytogenes continue to raise major public health concerns worldwide. In the United States alone, the centers for disease control and prevention have confirmed the occurrence of 183 cases of listeriosis with 39 fatalities within the last 3 years. Standard methods for the detection of pathogenic strains require up to 7 days to yield results, thus faster techniques with the same level of reliability for bacteria detection are desirable. This study reports on the development of a rapid, accurate, and sensitive electrochemical biosensor for rapid testing of Listeria spp. based on the selective binding of InlA aptamers to internalins in the cell membrane of the target bacteria. Hybrid nanomaterial platforms based on reduced graphene oxide and nanoplatinum were deposited onto Pt/Ir electrodes for enhancing electrochemical transduction during the recognition events. InlA aptamers were immobilized onto the nanomaterial platforms via metal-thiol adsorption. Aptamer loading onto different platform nanostructures was investigated through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The detection mechanism was evaluated by recording the electrochemical response to several bacterial dilutions in PBS buffer using the non-pathogenic species Listeria innocua. These preliminary results show that the aptasensor can be tuned for detection of Listeria concentrations as low as 100 CFU/ml in less than 3 hours (including incubation time and data analysis). The developed aptasensor opens a promising direction for rapid testing of Listeria monocytogenes in food products.

  12. Characterization of N2-fixing plant growth promoting endophytic and epiphytic bacterial community of Indian cultivated and wild rice (Oryza spp.) genotypes.

    Science.gov (United States)

    Banik, Avishek; Mukhopadhaya, Subhra Kanti; Dangar, Tushar Kanti

    2016-03-01

    The diversity of endophytic and epiphytic diazotrophs in different parts of rice plants has specificity to the niche (i.e. leaf, stem and root) of different genotypes and nutrient availability of the organ. Inoculation of the indigenous, polyvalent diazotrophs can facilitate and sustain production of non-leguminous crops like rice. Therefore, N2-fixing plant growth promoting bacteria (PGPB) were isolated from different parts of three Indian cultivated [Oryza sativa L. var. Sabita (semi deep/deep water)/Swarna (rain fed shallow lowland)/Swarna-Sub1(submergence tolerant)] and a wild (O. eichingeri) rice genotypes which respond differentially to nitrogenous fertilizers. Thirty-five isolates from four rice genotypes were categorized based on acetylene reduction assay on nitrogenase activity, biochemical tests, BIOLOG and 16S rRNA gene sequencing. The bacteria produced 9.36-155.83 nmole C2H4 mg(-1) dry bacteria h(-1) and among them nitrogenase activity of 11 potent isolates was complemented by nifH-sequence analysis. Phylogenetic analysis based on 16S rDNA sequencing divided them into five groups (shared 95-100 % sequence homology with type strains) belonging to five classes-alpha (Ancylobacter, Azorhizobium, Azospirillum, Rhizobium, Bradyrhizobium, Sinorhizobium, Novosphingobium, spp.), beta (Burkholderia sp.), gamma (Acinetobacter, Aeromonas, Azotobacter, Enterobacter, Klebsiella, Pantoea, Pseudomonas, Stenotrophomonas spp.) Proteobacteria, Bacilli (Bacillus, Paenibacillus spp.) and Actinobacteria (Microbacterium sp.). Besides, all bacterial strains possessed the intrinsic PGP traits of like indole (0.44-7.4 µg ml(-1)), ammonia (0.18-6 mmol ml(-1)), nitrite (0.01-3.4 mol ml(-1)), and siderophore (from 0.16-0.57 μmol ml(-1)) production. Inoculation of rice (cv. Swarna) seedlings with selected isolates had a positive impact on plant growth parameters like shoot and root elongation which was correlated with in vitro PGP attributes. The results indicated that the

  13. Efficacy of Trichoderma spp. against Common Fungal Pathogens

    Directory of Open Access Journals (Sweden)

    Tančić-Živanov Sonja

    2017-01-01

    Full Text Available Nowadays, organic food production demands more environmental friendly control of plant diseases. Biocontrol based on Trichoderma spp. usage is a promising due to Trichoderma is aggressive to broad range of phytopathogenic fungi. Given that, the aim of this study was to test in vitro antagonistic ability of ten native Serbian Trichoderma strains to ten common fungal pathogens. Study confirmed that Trichoderma spp. inhibits radial growth of Ascochyta pinodella (76.9%, A. pinodes (60.0%, A. pisi (68.5%, Fusarium graminearum (71.1%, F. proliferatum (63.9%, F. verticillioides (62.6%, F. oxysporum (63.9%, Macrophomina phaseolina (63.8%, and Pyrenophora teres (83.9%. These are first reports of Trichoderma spp. in vitro efficacy against A. pisi, A. pinodes, A. pinodella and P. teres. The lowest inhibitory effect was registered in dual cultures with Sclerotinia sclerotiorum - 52.2%.

  14. Hemorrhagic stroke secondary to Bothrops spp. venom: A case report.

    Science.gov (United States)

    Delgado, Anna Beatriz Temoteo; Gondim, Caio Cesar Vaz Lacet; Reichert, Lucas Pereira; da Silva, Pedro Hugo Vieira; Souza, Rodrigo Marmo da Costa E; Fernandes, Thiago Monteiro de Paiva; Calvo, Bernardino Fernandez

    2017-06-15

    The Bothrops spp. venom contain metalloproteinases that contributes to vascular and hemorrhagic effects. This case report describes a 58 years-old patient from the city of Dona Inês, Paraiba, Brazil victim of an ophidian accident by Bothrops spp. The vascular and hemorrhagic effects of venom components have triggered a hemorrhagic stroke. Brazil has about 600 deaths annually due to ophidian accidents. However, as reports have been precarious, the obtaining of epidemiological-clinical data has been affected. This case highlights the importance of prior knowledge of possible neurological and vascular complications in Bothrops spp. venom to increase the effectiveness of an adequate treatment. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. Frecuencia de aislamiento de Staphylococcus spp meticilina resistentes y Enterococcus spp vancomicina resistentes en hospitales de Cuba Frequency of methicilline-resistant Staphylococcus spp and vancomycin-resistant Enterococcus spp isolates in Cuban hospitals

    Directory of Open Access Journals (Sweden)

    Leonora González Mesa

    2005-12-01

    Full Text Available La resistencia a meticilina en el género Staphylococcus spp es un problema creciente en el ámbito mundial. La producción de una PBP alterada (PBP2a con baja afinidad a betalactámicos, mediada por el gen mec A, es la responsable de esta resistencia. Mientras que los Staphylococcus spp todavía permanecen sensibles a vancomicina, algunos Enterococcus spp han adquirido la capacidad de neutralizar esta droga. En nuestro país no se conocen datos actualizados sobre la tasa de infección por S. aureus meticilina resistente (SAMR, ni sobre la circulación de este germen en la comunidad, tampoco existen reportes de Enterococcus spp vancomicina resistente (EVR. En este estudio fueron analizadas 774 cepas, colectadas en hospitales del país. Se determinó el mecanismo de resistencia utilizando métodos sugeridos por las guías NCCLS. El 9.3 % (23 de los S. aureus aislados en los hospitales y 4.0% (7 S. aureus aislados en la comunidad, fueron SAMR, portadores del gen mec A, el 69.9 % (72 de Staphylococcus coagulasa negativo, fueron resistentes a oxacilina. En la detección del Enterococcus spp vancomicina resistente (EVR, se encontró una cepa portadora de este fenotipo. Nuestros resultados revelan que en nuestro país los SAMR no son un problema en los hospitales, ni en el ambiente comunitario, a pesar de que se reporta por primera vez la circulación de estos en la comunidad y la circulación de EVR en el ambiente hospitalario, su frecuencia es muy baja lo que refleja los avances obtenidos en la aplicación de políticas encaminadas a racionalizar el uso y consumo de antibióticos.Resistance to methicilline in Staphylococcus spp genus is a growing problem worldwide. The production of an altered penicillin-fixing protein with low mecA gen-mediated affinity to beta-lactams is responsible for this resistance. Although Staphylococcus spp still remain susceptible to vancomycin, some Enterococcus spp have acquired the capacity of neutralizing this drug. In

  16. PCR detection of Bartonella spp. in the dog

    Directory of Open Access Journals (Sweden)

    Jarmila Konvalinová

    2014-01-01

    Full Text Available Our study aimed at using PCR to identify the incidence of Bartonella spp. in blood of dogs. Altogether 286 dogs of 92 breeds aged 3 month to 17 years were tested from October 2008 to December 2009. Healthy dogs as well as dogs with various clinical symptoms of disease were included in the group. Samples were tested by polymerase chain reaction (PCR specific for the presence of Bartonella spp. Following the DNA examination in 286 dogs by PCR and subsequent sequencing, two samples were identified as Bartonella henselae (0.7%. Other species of Bartonella were not found. It was the first time in the Czech Republic when incidence of Bartonella spp. was determined in dogs.

  17. SPPTOOLS: Programming tools for the IRAF SPP language

    Science.gov (United States)

    Fitzpatrick, M.

    1992-01-01

    An IRAF package to assist in SPP code development and debugging is described. SPP is the machine-independent programming language used by virtually all IRAF tasks. Tools have been written to aide both novice and advanced SPP programmers with development and debugging by providing tasks to check the code for the number and type of arguments in all calls to IRAF VOS library procedures, list the calling sequences of IRAF tasks, create a database of identifiers for quick access, check for memory which is not freed, and a source code formatter. Debugging is simplified since the programmer is able to get a better understanding of the structure of his/her code, and IRAF library procedure calls (probably the most common source of errors) are automatically checked for correctness.

  18. Test structures for accurate UHF C-V measurements of nano-scale CMOSFETs with HfSiON and TiN metal gate

    NARCIS (Netherlands)

    Lee, Kyong-Taek; Schmitz, Jurriaan; Brown, George A.; Heh, Dawei; Choi, Rino; Harris, Rusty; Song, Seung-Chul; Lee, Byoung Hun; Han, In-Sikh; Lee, Hi-Deok; Jeong, Yoon-Ha

    2007-01-01

    Test structures for accurate UHF capacitance –voltage (C-V) measurements of high performance CMOSFETs with Hf-based high-k dielectric and TiN metal gate are analyzed. It is shown that series resistance or substrate resistance between the channel region and body contact plays a role in UHF C-V

  19. Isolation and characterization of a virus (CvV-BW1 that infects symbiotic algae of Paramecium bursaria in Lake Biwa, Japan

    Directory of Open Access Journals (Sweden)

    Kasahara Masahiro

    2010-09-01

    Full Text Available Abstract Background We performed an environmental study of viruses infecting the symbiotic single-celled algae of Paramecium bursaria (Paramecium bursaria Chlorella virus, PBCV in Lake Biwa, the largest lake in Japan. The viruses detected were all Chlorella variabilis virus (CvV = NC64A virus. One of them, designated CvV-BW1, was subjected to further characterization. Results CvV-BW1 formed small plaques and had a linear DNA genome of 370 kb, as judged by pulsed-field gel electrophoresis. Restriction analysis indicated that CvV-BW1 DNA belongs to group H, one of the most resistant groups among CvV DNAs. Based on a phylogenetic tree constructed using the dnapol gene, CvV was classified into two clades, A and B. CvV-BW1 belonged to clade B, in contrast to all previously identified virus strains of group H that belonged to clade A. Conclusions We conclude that CvV-BW1 composes a distinct species within C. variabilis virus.

  20. High floral bud abscission and lack of open flower abscission in Dendrobium cv. Miss Teen: rapid reduction of ethylene sensitivity in the abscission zone

    NARCIS (Netherlands)

    Bunya-atichart, K.; Ketsa, S.; Doorn, van W.G.

    2006-01-01

    We studied the abscission of floral buds and open flowers in cut Dendrobium inflorescences. Abscission of floral buds was high and sensitive to ethylene in all cultivars studied. Many open flowers abscised in most cultivars, but cv. Willie exhibited only small amount of floral fall and cv. Miss Teen

  1. High Prevalence of Anaplasma spp. in Small Ruminants in Morocco.

    Science.gov (United States)

    Ait Lbacha, H; Alali, S; Zouagui, Z; El Mamoun, L; Rhalem, A; Petit, E; Haddad, N; Gandoin, C; Boulouis, H-J; Maillard, R

    2017-02-01

    The prevalence of infection by Anaplasma spp. (including Anaplasma phagocytophilum) was determined using blood smear microscopy and PCR through screening of small ruminant blood samples collected from seven regions of Morocco. Co-infections of Anaplasma spp., Babesia spp, Theileria spp. and Mycoplasma spp. were investigated and risk factors for Anaplasma spp. infection assessed. A total of 422 small ruminant blood samples were randomly collected from 70 flocks. Individual animal (breed, age, tick burden and previous treatment) and flock data (GPS coordinate of farm, size of flock and livestock production system) were collected. Upon examination of blood smears, 375 blood samples (88.9%) were found to contain Anaplasma-like erythrocytic inclusion bodies. Upon screening with a large spectrum PCR targeting the Anaplasma 16S rRNA region, 303 (71%) samples were found to be positive. All 303 samples screened with the A. phagocytophilum-specific PCR, which targets the msp2 region, were found to be negative. Differences in prevalence were found to be statistically significant with regard to region, altitude, flock size, livestock production system, grazing system, presence of clinical cases and application of tick and tick-borne diseases prophylactic measures. Kappa analysis revealed a poor concordance between microscopy and PCR (k = 0.14). Agreement with PCR is improved by considering microscopy and packed cell volume (PCV) in parallel. The prevalence of double infections was found to be 1.7, 2.5 and 24% for Anaplasma-Babesia, Anaplasma-Mycoplasma and Anaplasma-Theileria, respectively. Co-infection with three or more haemoparasites was found in 1.6% of animals examined. In conclusion, we demonstrate the high burden of anaplasmosis in small ruminants in Morocco and the high prevalence of co-infections of tick-borne diseases. There is an urgent need to improve the control of this neglected group of diseases. © 2015 Blackwell Verlag GmbH.

  2. Electroacupuncture stimulation at CV4 prevents ovariectomy-induced osteoporosis in rats via Wnt-β-catenin signaling.

    Science.gov (United States)

    Fan, Huailing; Ji, Feng; Lin, Ying; Zhang, Mulan; Qin, Wei; Zhou, Qi; Wu, Qiang

    2016-03-01

    The present study aimed to investigate the effect of electroacupuncture stimulation at CV4 (also termed Guanyuan) on femoral osteocalcin also termed bone gla protein (BGP), alkaline phosphatase (ALP), bone mineral density (BMD) and biomechanics, as well as the Wnt‑β‑catenin signaling pathway in rats with postmenopausal osteoporosis. Female Sprague‑Dawley rats (4.5‑months old) were randomly divided into sham, Ovx, CV4 and mock groups (n=10/group). With the exception of those in the sham group, the rats were ovariectomized to induce postmenopausal osteoporosis. The rats in the CV4 and mock groups were given electroacupuncture at CV4 and non‑acupoint, respectively. The rats in the Ovx model and sham groups underwent identical fixing procedures, but did not undergo electroacupuncture. Following treatment, hematoxylin and eosin staining was used to observe morphological changes in the left femoral trabecular bone, and a three‑point‑bending test was used to analyze femur biomechanics and determine the BMD. In addition, an enzyme‑linked immunosorbent assay was used to measure the serum levels of ALP/BGP and reverse transcription‑quantitative polymerase chain reaction was used detect the expression levels of Wnt3a, β‑catenin and Runx2. In the present study, it was demonstrated that electroacupuncture at CV4 significantly improved the osteoporotic morphological changes that occurred in the ovariectomized rats, increased serum ALP and BGP levels, enhanced the maximum and fracture loads, increased BMD (Posteoporosis, possibly by activating the Wnt‑β‑catenin signaling pathway.

  3. Studies on stem cuttings of kiwi (Actinidia chinensis PL. CV Bruno

    Directory of Open Access Journals (Sweden)

    Elizabeth O. Ono

    2000-01-01

    Full Text Available The work was carried out to study the effects of some auxins and boron in the rooting of kiwi (Actinidia chinensis Planch cv Bruno stem cuttings.. These cuttings were treated on the base individually with H2O; NAA 300 mg.L-1; IBA 300 mg.L-1; NAA 300 mg.L-1 + Boron; IBA 300 mg.L-1 + Boron; NAA 0,5%-talc and IBA 0,5%-talc. After the treatments, the cuttings were placed in styrofoam trays with vermiculite under moist conditions for 120 days. The evaluation of auxin and boric acid effects were made by observing rooted stem cuttings percentage; reducing and total sugar analysis (g/100 g of dry matter; and tryptophan analysis (in µg/100 mg of dry matter. The effects of such treatments were observed during four seasons of the year. The results showed that summer season was the best for rooting. Use of IBA or NAA in the cuttings showed to be unnecessary.O presente trabalho teve como objetivo, estudar o efeito de auxinas sintéticas e do boro, sobre o enraizamento de estacas caulinares de kiwi (Actinidia chinensisPlanch. cv Bruno. As estacas continham dois nós com aproximadamente 10 cm de comprimento, contendo 2 folhas cortadas ao meio. As bases das estacas receberam os seguintes tratamentos: control (H2O; NAA 300 mg.L-1; IBA 300 mg.L-1; NAA 300 mg.L-1 + B; IBA 300 mg.L-1 + B; NAA 0,5%-pó e IBA 0,5%-pó. Após os tratamentos as estacas foram plantadas em bandejas de enraizamento contendo vermiculita pura e colocadas em câmara de nebulização por 120 dias até a coleta das mesmas. Para a avaliação do efeito das auxinas e boro, foram realizadas as seguintes observações: 1. porcentagem de estacas enraizadas; 2. análise de açúcares redutores e açúcares totais (em g/100 g de matéria seca; 3. análise de triptofano (em µg/100 mg de matéria seca. Além disso, foram verificados o efeito dos tratamentos em quatro épocas, que corresponderam às estações do ano (primavera, verão, outono e inverno. Através dos resultados obtidos no processo de

  4. SPP propagation in nonlinear glass-metal interface

    KAUST Repository

    Sagor, Rakibul Hasan

    2011-12-01

    The non-linear propagation of Surface-Plasmon-Polaritons (SPP) in single interface of metal and chalcogenide glass (ChG) is considered. A time domain simulation algorithm is developed using the Finite Difference Time Domain (FDTD) method. The general polarization algorithm incorporated in the auxiliary differential equation (ADE) is used to model frequency-dependent dispersion relation and third-order nonlinearity of ChG. The main objective is to observe the nonlinear behavior of SPP propagation and study the dynamics of the whole structure. © 2011 IEEE.

  5. An Ectopic Case of Tunga spp. Infection in Peru

    Science.gov (United States)

    Maco, Vicente; Maco, Vicente P.; Gotuzzo, Eduardo

    2010-01-01

    Tungiasis is a neglected ectoparasitism of impoverished areas in South America and sub-Saharan Africa. The sand flea Tunga spp. preferably infests the soles and the periungueal and interdigital regions of the feet. Ectopic tungiasis is rare, even in highly endemic areas. We describe a case of an indigenous patient in Peru who presented with a nodular lesion in the extensor aspect of the knee and whose biopsy was compatible with Tunga spp. This is the first documented case of knee tungiasis in an endemic country. The historical, clinical, histological, and current epidemiological aspects of tungiasis in Peru are discussed here. PMID:20519602

  6. [Isolation of Candida spp. from ascites in cirrhotic patients].

    Science.gov (United States)

    Saludes, Paula; Araguás, Cristina; Sánchez-Delgado, Jordi; Dalmau, Blai; Font, Bernat

    2016-10-01

    The isolation of Candida spp. in ascites of cirrhotic patients is an uncommon situation in clinical practice. Factors that have been associated with increased susceptibility to primary fungal peritonitis are exposure to broad-spectrum antibiotics and immunosuppression, a typical situation of these patients. We report seven episodes of Candida spp. isolation in ascites of cirrhotic patients detected in our hospital during the past 15years. Copyright © 2015 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.

  7. High prevalence of Leptospira spp. in sewer rats (Rattus norvegicus)

    DEFF Research Database (Denmark)

    Krøjgaard, L H; Villumsen, S; Markussen, M D K

    2009-01-01

    Earlier studies on the ecology of leptospirosis in temperate regions focused mainly on free-ranging rats in rural areas. Here we report on the occurrence of Leptospira spp. in Rattus norvegicus living in sewers in a suburban area in Copenhagen, Denmark. In 2006-2007, about 30 rats were captured...... in sewers at each of six different locations. Rat kidneys were screened by PCR for pathogenic Leptospira spp. In one location no infected rats were found, whereas the prevalence in the remaining five locations ranged between 48% and 89%. Micro-agglutination tests showed that serogroup Pomona, Sejroe...

  8. Brunfelsia spp (yesterday, today, tomorrow) toxicity in four dogs.

    Science.gov (United States)

    Singh, M; Cowan, S; Child, G

    2008-06-01

    Four dogs were treated for acute toxicity following ingestion of the popular garden shrub 'Yesterday, today, tomorrow' (Brunfelsia spp). Clinical signs included vomiting, diarrhoea, muscle tremors, anxiousness, opisthotonus and seizures. All dogs recovered following treatment with any or all of general anaesthetic, gastric lavage, enema, diazepam, phenobarbitone or propofol sedation. Brunfelsia spp toxicity should be considered in young, previously healthy dogs presenting with gastrointestinal signs that rapidly progress to muscle tremors and seizures. Examination of faeces was required for diagnosis in all cases. Owners should also be questioned thoroughly about their dogs' access to such plants.

  9. HUBUNGAN MASA KERJA DAN SIKAP KERJA DENGAN KEJADIAN SINDROM KARPAL PADA PEMBATIK CV. PUSAKA BERUANG LASEM

    Directory of Open Access Journals (Sweden)

    Cris Purwandari Mulyawati Agustin

    2014-10-01

    Full Text Available Pembatik merupakan pekerja sektor informal, dimana pada saat membatik melakukan gerakan berulang, gerakan tangan dengan kekuatan, posisi fleksi dan ekstensi, sehingga menyebabkan stress pada jaringan disekitar terowongan karpal. Tujuan penelitian ini untuk mengetahui hubungan masa kerja dan sikap kerja dengan kejadian STK pada pembatik CV. Pusaka Beruang.Penelitian ini menggunakan pendekatan cross sectional. Populasi dalam penelitian berjumlah 68 orang. Sampel penelitian berjumlah 22 responden. Instrument penelitian berupa kuesioner dan pengukuran tes phalen. Data dianalisis dengan uji Chi-square.Hasil penelitian menunjukkan bahwa ada hubungan masa kerja p=0,029 dan sikap kerja p=0,031 dengan kejadian STK. Masa kerja > 4 tahun dan level sikap kerja yang tinggi mempunyai risiko STK karena terjadi stress disekitar jaringan terowongan karpal.Simpulan pada penelitian ini ada hubungan antara masa kerja dan sikap kerja dengan kejadian STK. Saran untuk pekerja diharapkan dapat melakukan istirahat secara teratur setiap 15-20 menit untuk mengurangi risiko STK. Batik is an informal sector workers describe or designed batik with the traditional way of working. Which time both do repetitive motion, hand movements with strength, position, position the extension and flexion, hand static, thus causing stress on the network around the carpal tunnel. The purpose of this research is to know the relation tenure and working attitude with STK batik meker in CV. Pusaka Beruang. This research uses the approach of cross sectional. The population in this research is totalling 68 peoples. Sample research amounted to 22 respondents. Research Instrument in the form of questionnaires and measurement test phalen. Data were analyzed by Chi square test. The results showed that there was a relationship tenure p = 0.029 and working attitude p = 0.031 with STK events. Tenure > 4 years and a high level of work attitudes have STK risk due to stress around the carpal tunnel syndrome

  10. Occurrence of anti-Leptospira spp. antibodies in Rhipidomys spp. from a forest fragment of the Brazilian Cerrado.

    Science.gov (United States)

    Gomes, D O; Ramos, G B; Alves, V B A; Ciuffa, A Z; Cuccato, L P; Dos Reis, T F M; Lima, A M C; Gonçalves, M C; Tolesano, G V; Rodrigues, V S; Szabó, M P J

    2017-03-01

    Leptospirosis is a zoonotic disease of world importance, and its transmission depends on the interaction between humans and animals. Given the necessity to investigate potential hosts of Leptospira spp., this study verified the prevalence of different serovars in the species of Rhipidomys spp., a widespread sigmodont rodent in Brazil. The studied population originates from a semi-evergreen forest located in the county of Uberlândia, in the state of Minas Gerais. The microscopic agglutination test (MAT) was performed with 14 serovars. Thirteen out of the 43 wild rodents captured showed a positive agglutination reaction, with a greater prevalence of the serovars Pyrogenes, Copenhageni, and Canicola. This study found a prevalence of 30.3% anti-Leptospira spp. antibodies; all positive animals were reactive to more than one serovar.

  11. Competitive nodulation blocking of cv. Afghanistan pea is related to high levels of nodulation factors made by some strains of Rhizobium leguminosarum bv. viciae.

    Science.gov (United States)

    Hogg, Bridget; Davies, Andrea E; Wilson, Karen E; Bisseling, Ton; Downie, J Allan

    2002-01-01

    Cultivar Afghanistan peas are resistant to nodulation by many strains of Rhizobium leguminosarum bv. viciae but are nodulated by strain TOM, which carries the host specificity gene nodX. Some strains that lack nodX can inhibit nodulation of cv. Afghanistan by strain TOM. We present evidence that this "competitive nodulation-blocking" (Cnb) phenotype may result from high levels of Nod factors inhibiting nodulation of cv. Afghanistan peas. The TOM nod gene region (including nodX) is cloned on pIJ1095, and strains (including TOM itself) carrying pIJ1095 nodulate cv. Afghanistan peas very poorly but can nodulate other varieties normally. The presence of pIJ1095, which causes increased levels of Nod factor production, correlates with Cnb. Nodulation of cv. Afghanistan by TOM is also inhibited by a cloned nodD gene that increases nod gene expression and Nod factor production. Nodulation of cv. Afghanistan can be stimulated if nodD on pIJ1095 is mutated, thus severely reducing the level of Nod factor produced. Repression of nod gene expression by nolR eliminates the Cnb phenotype and can stimulate nodulation of cv. Afghanistan. Addition of Nod factors to cv. Afghanistan roots strongly inhibits nodulation. The Cnb+ strains and added Nod factors inhibit infection thread initiation by strain TOM. The sym2A allele determines resistance of cv. Afghanistan to nodulation by strains of R. leguminosarum bv. viciae lacking nodX. We tested whether sym2A is involved in Cnb by using a pea line carrying the sym2A region introgressed from cv. Afghanistan; nodulation in the introgressed line was inhibited by Cnb+ strains. Therefore, the sym2A region has an effect on Cnb, although another locus (or loci) may contribute to the stronger Cnb seen in cv. Afghanistan.

  12. Manutenção da qualidade de rosas cortadas cv. Vega em soluções conservantes Keeping quality of cut roses cv. Vega in holding solutions

    Directory of Open Access Journals (Sweden)

    Júlia de Pietro

    2012-03-01

    Full Text Available A rosa é considerada uma das flores de corte mais tradicionais e requisitadas no mercado mundial. Entretanto, após o corte, essas flores tornam-se altamente perecíveis devido à elevada atividade metabólica que conduz aos processos de senescência. Assim, o experimento foi realizado para verificar o efeito do uso de soluções conservantes na fisiologia pós-colheita de rosas vermelhas cortadas cultivar Vega. Utilizou-se o delineamento inteiramente casualizado, em esquema fatorial composto por dois fatores: sete tratamentos pós-colheita e quatro datas de avaliação. As flores permaneceram nas seguintes soluções de manutenção: 1 Água destilada; 2 8-hidroxiquinolina (200 mg L-1; 3 8-hidroxiquinolina (200 mg L-1 + Sacarose (20 mg L-1; 4 Ácido cítrico (75 mg L-1; 5 Ácido cítrico (75 mg L-1 + Sacarose (20 mg L-1; 6 6-benziladenina (60 mg L-1; 7 6 benziladenina (60 mg L-1 + Sacarose (20 mg L-1. Foram realizadas análises qualitativas e fisiológicas ao longo do período de avaliação. O tratamento com 8-hidroxiquinolina e ácido cítrico revelaram-se mais promissores para manter a qualidade e prolongar a vida de vaso das flores, o que foi evidenciado pela menor perda de massa fresca, atividade respiratória e conteúdo relativo de água; melhor manutenção do conteúdo de carboidratos solúveis e redutores, antocianina e coloração.The rose is considered one of the most traditional and required cut flowers in the world market. However, after cutting, these flowers become highly perishable, because the high metabolic activity leads to senescence processes. The experiment was accomplished to verify the effect of holding solutions on the postharvest physiology of cut red roses, cv. Vega. A randomized design in a factorial scheme composed by two factors was used: seven postharvest treatments and four evaluation dates. The flowers remained in the following holding solutions: 1 Distilled water; 2 8-hydroxyquinoline citrate (200 mg L-1; 3 8

  13. Controle do amadurecimento do kiwi cv. monty com 1-metilciclopropeno The use of 1-methylcyclopropene in ripening control of kiwi cv. monty

    Directory of Open Access Journals (Sweden)

    Julius Blum

    2009-03-01

    Full Text Available O 1-metilciclopropeno (1-MCP é um gás que atua inibindo o sítio de ação do etileno, aumentando o tempo de armazenamento de frutos. O objetivo deste trabalho foi avaliar o efeito deste gás na conservação pós-colheita de kiwi 'Monty', à temperatura ambiente. Ele consistiu na exposição dos frutos a concentrações de 0; 0,5 e 1,0 mg dm-3 de 1-MCP, durante 24 horas, e posterior estocagem a 20 ± 4 ºC, por até 20 dias, com avaliações a cada 4 dias. Avaliaram-se: firmeza, translucidez da polpa, sólidos solúveis (SS, pH, acidez titulável (AT, pectina, % de frutos firmes para transporte e consumo. A translucidez da polpa mostrou-se um parâmetro adequado para monitoramento do amadurecimento, visto que houve correlação com a firmeza. A aplicação de 1-MCP preservou a firmeza e preveniu a translucidez da polpa, prolongando a qualidade para transporte e consumo por 4 e 12 dias, respectivamente, e não teve efeito sobre o pH, SS e pectinas. Nos tratamentos com 1-MCP, ocorreu um aumento inicial na acidez, mas com o passar do tempo, ocorreu diminuição da mesma, da firmeza e aumento dos sólidos solúveis e da translucidez da polpa, caracterizando o amadurecimento dos frutos.The gas 1-methylcyclopropene acts inhibiting the action site of ethylene, increasing the postharvest period of the fruits. The aim of this work was to evaluate the effect of this gas on postharvest conservation of kiwifruit cv. Monty at ambient temperature. The study consisted of fruit exposition to 1MCP at the concentrations of 0, 0,5 and 1,0 mg dm-3 during 24 hours, and stored at 20 ± 4 ºC per 20 days, with evaluations at 4 days intervals. It had been evaluated: firmness of pulp, pulp color, soluble solids, pH, total acidity, % of pectin and % of firm fruits for transport and consumption. The color of pulp was an adequate parameter to evaluate the ripening of fruits, since it had correlation with the firmness. The application of 1MCP preserved the firmness the

  14. Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua.

    Science.gov (United States)

    Marcenaro, Delfia; Valkonen, Jari P T

    2016-01-01

    Common bean (Phaseolus vulgaris L.) is an important legume with high nutritional value. In Nicaragua, certified healthy seeds of local bean varieties are not available, and seedborne fungi have gained little attention. Here, were surveyed seedborne pathogenic fungi in an important local bean cultivar, 'INTA Rojo'. Beans grown in the four main production areas in Nicaragua (Boaco, Carazo, Estelí, Matagalpa) for future use as seed stock were sampled from four seed storehouses and six seed lots. A total of 133 fungal strains were isolated from surface-sterilized beans and inoculated to healthy lima beans (Phaseolus lunatus) under controlled conditions. Eighty-seven isolates caused symptoms of varying severity in the seedlings, including discoloration, necrotic lesions, cankers, rot, and lethal necrosis. Pathogenic isolates were divided into eight phenotypically distinguishable groups based on morphology and growth characteristics on artificial growth medium, and further identified by analysis of the internal transcribed spacer sequences (ITS1 and ITS2) of the ribosomal RNA genes. The pathogenic isolates belonged to eight genera. Fusarium spp. (F. chlamydosporum, F. equiseti, F. incarnatum), Lasiodiplodia theobromae, Macrophomina phaseolina, and Penicillium citrinum were the most damaging and common fungi found in the seed lots. Furthermore, Corynespora cassiicola, Colletotrichum capsisi, Colletotrichum gloeosporioides, Aspergillus flavus, and Diaporthe sp. (Phomopsis) were seedborne in cultivar 'INTA Rojo' and found to be pathogenic to bean seedlings. This study reveals, for the first time, many seedborne pathogenic fungi in beans in Nicaragua; furthermore, prior to this study, little information was available concerning F. equiseti, F. incarnatum, L. theobromae, C. cassiicola, and Diaporthe spp. as seedborne pathogens of common bean. Our results lay the basis for developing diagnostic tools for seed health inspection and for further study of the epidemiology

  15. IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea PROPAGAÇÃO IN VITRO DE MUSSAENDA (Mussaenda erythrophylla cv. Rosea

    Directory of Open Access Journals (Sweden)

    Alexandre Bosco de Oliveira

    2010-05-01

    Full Text Available

    This research aimed to develop a protocol for the micropropagation of Mussaenda erytrophylla cv. Rosea. Calcium hypochlorite or sodium hypochlorite (2.5% was used for the sterilization of bracts segments, with or without previous immersion in alcohol 70%. For callus induction, bracts segments were inoculated on MS medium with different concentrations of BAP and NAA. For buds induction, callus were inoculated on MS medium with different concentrations of cytokinins BAP, 2iP, and KIN (0.0 mg L-1, 0.5 mg L-1, 1.0 mg L-1, and 2.0 mg L-1. For multiplication, nodal segments from in vitro plants were inoculated in MS medium with BAP, KIN, and 2iP, in the concentrations of 0.0 mg L-1, 0.5 mg L-1, 1.0 mg L-1, and 2.0 mg L-1. For rooting, nodal segments from in vitro plants were inoculated using two MS medium dilutions: full strength and half strength of MS basal salt mixture (macronutrients salts and different concentrations of IBA (0.00 mg L-1, 0.25 mg L-1, 0.50 mg L-1, and 1.00 mg L-1. The best sterilization treatment was achieved by using calcium hypochlorite combined with immersion in alcohol. For callus induction, the MS medium with 0.4 mg L-1 of NAA plus 4.0 mg L-1 of BAP was more efficient. Bud multiplication was improved in MS medium with 0.5 mg L-1 of 2iP or 1.0 mg L-1 of KIN. The highest percentage of explants with roots without emission of callus was observed in the medium with 50% and 100% of MS salts without IBA.

  16. Resistance to Antibiotics in Strains of Staphylococcus spp., Enterococcus spp. and Escherichia coli Isolated from Rectal Swabs of Pigs

    Directory of Open Access Journals (Sweden)

    M. Kolář

    2008-01-01

    Full Text Available The study aimed at determining the level of resistance of selected bacterial species (Staphylococcus spp., Enterococcus spp., Escherichia coli isolated from rectal swabs of pigs to antimicrobial agents. The tested strains were isolated from piglets aged 7 to 30 days. Bacterial species were identified by standard microbiological techniques and susceptibility to antibiotics was determined quantitatively by the standard microdilution method. Resistance of the Staphylococcus aureus strain to oxacillin was confirmed by detection of the mecA gene and PBP2a. A total of 115 Staphylococcus spp. isolates were collected. In the case of Staphylococcus aureus, the methicillin-resistant strain (MRSA was identified. Moreover, higher frequency of coagulase-negative staphylococci with minimum inhibitory concentration of oxacillin ≥ 0.5 mg/l was noticed. Inducible resistance to clindamycin in the Staphylococcus hominis strain was also detected. The strains of Enterococcus spp. (61 isolates exhibited high resistance to tetracycline (98.5%, erythromycin (86.8% and chloramphenicol (54.4%. Vancomycin-resistant enterococci were not isolated. In the case of Escherichia coli strains (111 isolates, higher frequency of resistant strains to tetracycline (81.1% and ampicillin (62.2% was documented. Resistance to fluoroquinolones and production of broad-spectrum β-lactamases was not noticed. The presented study may be considered as a pilot project assessing the prevalence of resistant bacteria in piglets kept on a single farm. It demonstrated the presence of resistant strains of Staphylococcus spp., including one MRSA strain, Enterococcus spp. and Escherichia coli. These strains may be present as a result of postnatal colonization with both bacterial microflora of dams and environmental microflora.

  17. Variables Associated with Infections of Cattle by Brucella abortus., Leptospira spp. and Neospora spp. in Amazon Region in Brazil.

    Science.gov (United States)

    Chiebao, D P; Valadas, S Y O B; Minervino, A H H; Castro, V; Romaldini, A H C N; Calhau, A S; De Souza, R A B; Gennari, S M; Keid, L B; Soares, R M

    2015-10-01

    The frequency of Neospora spp., Leptospira spp. and Brucella abortus infections in adult cattle was determined in herds of the State of Pará, Brazil, which is an important region for cattle production located in the Amazon region. A total of 3466 adult female cattle from 176 herds were tested, leading to a frequency of seropositive animals of 14.7%, 3.7% and 65.5% and a herd positivity of 87.4%, 41.3% and 98.8% for infections caused by Neospora spp., B. abortus and Leptospira spp., respectively. The five most frequently diagnosed serologic responses to Leptospira spp. were those against serovars hardjo, wolfii, grippotyphosa, hebdomadis and shermani. The following associations were found: practice of artificial insemination, large farm size, large herd size, large number of dogs and high number of total abortions per year with the presence of antibodies against serovar hardjo; positive results to serovar grippotyphosa with the presence of dogs; inappropriate disposal of aborted foetuses with positivity to serovar hebdomadis. Serovar grippotyphosa was also associated with number of episodes of abortions. Neospora spp. positive herds were associated with episodes of abortion and B. abortus infection with the disposal of dead animals and aborted foetuses on pastures and with the use of artificial insemination. In conclusion, the high frequency of brucellosis, leptospirosis and neosporosis in the region may be a consequence of social, natural and raising conditions as: (i) climate conditions that favour the survival and spread of pathogens in the environment; (ii) farms located in regions bordering forest areas; (iii) farms in areas of difficult access to the veterinary service; (iv) extensive beef herds raised at pastures with different age and productive groups inter-mingled; and (v) minimal concerns regarding hygiene practices and disease prevention measures. © 2013 Blackwell Verlag GmbH.

  18. Potencial mutualismo entre Cipocereus minensis (Werderm.) F.Ritter (Cactaceae) e Silvestritermes spp. e Nasutitermes spp. (Isoptera:Termitidae)

    OpenAIRE

    Souza, Julya Pires; Lopes, Sabrina Aparecida; Mendonça Filho, Carlos Victor; Santos, Thiago

    2017-01-01

    O objetivo foi verificar se a associação entre \\textit{Cipocereus minensis} e \\textit{Silvestritermes spp.} e \\textit{Nasutitermes spp.} influencia o sucesso reprodutivo do cacto, pois os cupinzeiros funcionam como manchas de nutrientes. Foram observados quinzenalmente 30 indivíduos de \\textit{C. minensis} divididos em 2 grupos: 19 indivíduos associados ao cupinzeiro; 11 indivíduos afixados em rochas. Foram empregados modelos lineares generalizados mistos onde o substrato, as temperaturas mé...

  19. Thermal degradation of anthocyanins from purple potato (cv. Purple Majesty) and impact on antioxidant capacity.

    Science.gov (United States)

    Nayak, Balunkeswar; Berrios, Jose De J; Powers, Joseph R; Tang, Juming

    2011-10-26

    Degradation parameters of purified anthocyanins from purple-fleshed potato (cv. Purple Majesty) heated at high temperatures (100-150 °C) were determined. Purified anthocyanins, prepared by removing salts, sugars, and colorless nonanthocyanin phenolics from the crude extract, were monitored and quantified using HPLC and spectrophotometry for heat-induced degradation products. Separation of colorless phenolics from the anthocyanins was confirmed using HPLC at two wavelengths, 280 and 520 nm. The degradation kinetics of purified anthocyanins followed a first-order reaction with reaction rate constants (k values) of 0.0262-0.2855 min(-1), an activation energy of 72.89 kJ/mol, thermal death times (D values) of 8.06-8789 min, and a z value of 47.84 °C over the temperature range of 100-150 °C. The enthalpy and entropy of activation were 59.97 kJ/mol and -116.46 J/mol·K, respectively. The antioxidant capacity in the purified anthocyanins, measured by DPPH and ABTS assays, was increased after the thermal treatment, indicating antioxidant activities of degradation products in the samples.

  20. [Screening of the anti-tumor active fraction from Ipomoea batatas Lam. (cv.simon) leaves].

    Science.gov (United States)

    Lü, Shuhe; Lin, Cong; Xu, Pingsheng

    2015-05-01

    Three fractions (SM, SM-A, SM-B) were prepared from different polarity parts of Ipomoea batatas Lam. (cv.simon) leaves and the anti-tumor potency as well as the dose-response relations were evaluated. The anti-tumor activities of fraction SM, SM-A or SM-B were screened by MTS in human hepatic cancer Hep3B cells, lung cancer A549 cells or gastric carcinoma MGC803 cells, respectively. The three fractions all showed anti-tumor activities in three cancer cells with different sensitivity. Among them, SM-B was the most potent fraction with IC50 values at 15.17 mg/L, 72.64 mg/L or 165.47 mg/L in MGC803 cells, A549 cells or Hep3B cells, respectively (P<0.05). Th e extraction of Brazil sweet potato leaves displayed anti-tumor activity and SM-B was the most potent fraction.

  1. An efficient Agrobacterium-mediated transformation of strawberry cv. Camarosa by a dual plasmid system.

    Science.gov (United States)

    Haddadi, Fatemeh; Aziz, Maheran Abd; Abdullah, Siti Nor Akmar; Tan, Soon Guan; Kamaladini, Hossein

    2015-02-23

    An Agrobacterium-mediated transformation method was applied to introduce the luciferase reporter gene under the control of the CaMV35S promoter in the pGreen0049 binary vector into strawberry cv. Camarosa. The in vitro regeneration system of strawberry leaves to be used in the transformation was optimized using different TDZ concentrations in MS medium. TDZ at 16 µM showed the highest percentage (100%) of shoot formation and the highest mean number of shoots (24) produced per explant. Studies on the effects of different antibiotics, namely timentin, cefotaxime, carbenicillin and ampicillin, on shoot regeneration of strawberry leaf explants showed the best shoot regeneration in the presence of 300 mg/L timentin and 150 mg/L cefotaxime. Assessment of the different factors affecting Agrobacterium mediated-transformation of strawberry with the luciferase gene showed the highest efficiency of putative transformant production (86%) in the treatment with no preculture, bacterial OD600 of 0.6 and the addition of 150 mg/L cefotaxime in the pre-selection and selection media. The presence of the luciferase gene in the plant genome was verified by the luciferase reporter gene assay, nested PCR amplification and dot blot of genomic DNA isolated from the young leaves of each putatively transformed plantlet.

  2. Impacts of strigolactone on shoot branching under phosphate starvation in chrysanthemum (Dendranthema grandiflorum cv. Jinba)

    Science.gov (United States)

    Xi, Lin; Wen, Chao; Fang, Shuang; Chen, Xiaoli; Nie, Jing; Chu, JinFang; Yuan, Cunquan; Yan, Cunyu; Ma, Nan; Zhao, Liangjun

    2015-01-01

    Chrysanthemum (Dendranthema grandiflorum cv. Jinba) shoot branching is determined by bud outgrowth during the vegetative growth stage. The degree of axillary bud outgrowth is highly influenced by environmental conditions, such as nutrient availability. Here, we demonstrated that phosphorus (Pi) starvation significantly reduces axillary bud outgrowth in chrysanthemum. A strigolactone (SL) biosynthesis gene, DgCCD7, was isolated and characterized as an ortholog of MAX3/DAD3/RMS5/D17. By using ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS), three putative SLs were identified and levels of all three SLs showed strong increase under Pi starvation conditions. Determinations of the distribution of SLs and regulation of DgCCD7/8 in response to Pi changes in root indicate that SL acts systemically. However, temporal expression patterns of biosynthesis and signaling genes in nodes revealed that Pi starvation causes a local response of SL pathway. Treatment of node segments with or without auxin and Pi revealed that in the absence of exogenous auxin, Pi delayed axillary buds outgrowth and up-regulated local SL pathway genes. These data indicated that an auxin-SL regulatory loop responded to Pi starvation for delaying bud outgrowth locally, root biosynthesized SLs were transported acropetally and functioned in shoot branching inhibition under Pi starvation. We proposed that SLs contributed to chrysanthemum shoot branching control in response to Pi-limiting conditions in a systemic way. PMID:26442011

  3. Occurrence of metaxenia and false hybrids in Brassica juncea L. cv. Kikarashina × B. napus

    Science.gov (United States)

    Tsuda, Mai; Konagaya, Ken-ichi; Okuzaki, Ayako; Kaneko, Yukio; Tabei, Yutaka

    2011-01-01

    Imported genetically modified (GM) canola (Brassica napus) is approved by Japanese law. Some GM canola varieties have been found around importation sites, and there is public concern that these may have any harmful effects on related species such as reduction of wild relatives. Because B. juncea is distributed throughout Japan and is known to be high crossability with B. napus, it is assumed to be a recipient of B. napus. However, there are few reports for introgression of cross-combination in B. juncea × B. napus. To assess crossability, we artificially pollinated B. juncea with B. napus. After harvesting a large number of progeny seeds, we observed false hybrids and metaxenia of seed coats. Seed coat color was classified into four categories and false hybrids were confirmed by morphological characteristics and random amplified polymorphic DNA (RAPD) markers. Furthermore, the occurrence of false hybrids was affected by varietal differences in B. napus, whereas that of metaxenia was related to hybridity. Therefore, we suggest that metaxenia can be used as a marker for hybrid identification in B. juncea L. cv. Kikarashina × B. napus. Our results suggest that hybrid productivity in B. juncea × B. napus should not be evaluated by only seed productivity, crossability ought to be assessed the detection of true hybrids. PMID:23136472

  4. The impact of high temperatures on Vitis vinifera cv. Semillon grapevine performance and berry ripening

    Directory of Open Access Journals (Sweden)

    Dennis H Greer

    2013-12-01

    Full Text Available The heat event that occurred in many parts of Australia in 2009 was the worst on record for the past decade, with air temperatures exceeding 40oC for 14 days. Our aim was to assess the impacts of this heat event on vine performance, including ripening, yield and gas exchange of Vitis vinifera cv. Semillon grown in a Riverina vineyard. To assess the affect of high temperatures on Semillon grapevines, the vines were covered with a protective layer to reduce radiant heating and were compared with vines exposed to ambient conditions. The heat event had major effects on ripening; reducing the rate by 50% and delaying harvest ripeness and causing a high incidence of berry shrivel and sunburn. Yield was not affected. Photosynthesis was reduced 35% by the heat event while transpiration increased nearly 3-fold and was accounted for by increased stomatal conductance. The conclusion of this study was that heat events delayed ripening in Semillon berries and caused a significant reduction in berry quality. Strategies to minimise the radiant load during heat events are required and this study has confirmed a protective layer can reduce canopy temperatures and enhance berry quality.

  5. PERDAS QUALITATIVAS DE MAÇÃS CV. GALA EM ARMAZENAMENTO REFRIGERADO

    Directory of Open Access Journals (Sweden)

    BENDER RENAR JOÃO

    2001-01-01

    Full Text Available O presente trabalho foi desenvolvido com o objetivo de dimensionar as perdas de qualidade de maçãs cv. Gala durante o armazenamento refrigerado. Para determinar estas perdas, foi feito um acompanhamento durante três anos consecutivos, com frutos de três tamanhos: calibre em torno de 100 frutos/caixa de 18kg, calibre 130 e calibre acima de 200. Quinzenalmente, durante os primeiros 90 dias de armazenagem no primeiro ano, 144 dias no segundo ano e 211 dias no terceiro ano, amostras de 20 a 30 maçãs foram avaliadas para perda de peso e parâmetros qualitativos como a firmeza de polpa, os sólidos solúveis totais (SST e acidez titulável. As variáveis que apresentaram as melhores correlações com o período de armazenagem e, portanto, podem auxiliar na estimativa de possíveis perdas quantitativas durante o armazenamento em ar-refrigerado foram a firmeza de polpa e a acidez titulável. As maiores perdas de qualidade, com exceção da firmeza de polpa, foram observadas em frutos dos tamanhos menores.

  6. Quebra de dormência de gemas de macieira cv. Eva tratadas com cianamida hidrogenada

    Directory of Open Access Journals (Sweden)

    CRUZ JÚNIOR ÁLVARO DE OLIVEIRA

    2002-01-01

    Full Text Available A maioria das cultivares de macieiras plantadas no Brasil apresentam deficiência e desuniformidade de brotação, devido à má distribuição e/ou baixo número de horas de frio abaixo de 7,2masculine C, constituindo-se num entrave à produtividade. Mesmo utilizando-se de cultivares de baixa exigência de frio invernal, como a cultivar Eva, tem-se verificado a necessidade de se fazer a quebra de dormência para uniformizar a brotação, compatibilizar o florescimento e antecipar e melhorar a produção em regiões com invernos amenos ou anos de baixo acúmulo de frio. Avaliou-se o efeito da aplicação de cianamida hidrogenada em três diferentes concentrações (0,5; 1,0 e 1,5 % associada ao óleo mineral (3% na brotação de gemas laterais e terminais de macieiras da cv. Eva, e a fenologia das cvs. Eva e Anabela. Concluiu-se que a concentração de cianamida hidrogenada influi na percentagem de gemas laterais e terminais brotadas, sendo a concentração de 1,5% de cianamida hidrogenada associada a 3% de óleo mineral a mais efetiva na quebra da dormência das gemas

  7. Evaluating the success of vegetative propagation techniques in loquat cv. mardan

    International Nuclear Information System (INIS)

    Abbasi, N.A.; Hafiz, I.A.; Qureshi, A.A.

    2014-01-01

    Loquat cv. Mardan is a promising variety in loquat growing areas of northern Punjab and Khyber Pakhtoon Khwa. Loquat growers face serious issue of true to type plants availability. An attempt was made to explore different vegetative propagation techniques to find out most successful method for nursery industry. In the study propagation through cutting (during spring) and grafting during different suitable seasons (spring and monsoon) was examined. Cuttings were treated with different growth regulators to promote rooting. PBZ, IBA and their combinations were applied to the cuttings. Highest success percentage (40%) was achieved with PBZ. This limited success in rooting of cuttings was complete failure in getting plant survival at the end of the study. In the second phase of the experiment different grafting techniques i.e. tongue, cleft and side grafting were evaluated during two different seasons i.e., spring and monsoon. Cleft grafting in the months of March/ April proved to be most successful method in terms of success (70%), days taken for shoot growth, no. of leaves, shoots length, no. of branches. While in monsoon season side grafting was significantly better with a success of 85%. Overall, monsoon side grafting proved to be more successful than any other method. (author)

  8. Biocontrol of gray mold on Rosa Hybrida cv. Baccara with Bacillus Subtilis

    Directory of Open Access Journals (Sweden)

    E. S. Mousavi

    2017-06-01

    Full Text Available The bacteria Bacillus subtilis was investigated for control of gray mold, postharvest quality and antioxidant enzymes of Rosa hybrida cv. Baccara. The results indicated that the treatment of Bacillus subtilis suspension of 1 × 108cfu mL−1 with resulted in a remarkably improved control of Botrytis cinerea infections. CAT activity in treated flower by antagonism were significantly more than those control (P ≤ 0.05 at 25◦C, RH 60-70%. POD activity cut flowers increased during the flower bud development with the lowest activity present at water-sprayed control. Enhanced by antagonism could be due to either induced resistance or direct effects of these chemicals on Botrytis. The proper concentration of Bacillus subtilis can thus provide an effective strategy to increase postharvest vase life of Rosa. Postharvest antagonism application prolonged vase-life in cut rose flowers by improving the reactive oxygen species (ROS scavenging capacity related to CAT and POD activity

  9. [Chemical quality of Chrysanthemum morifolium cv. 'Hangju' (HJ) induced by sulfur fumigation processing].

    Science.gov (United States)

    Wang, Shan; Hao, Li-Juan; Zhu, Jing-Jing; Zhang, Qi-Wei; Wang, Zhi-Min; Zhang, Xian; Lu, Tu-Lin

    2014-04-01

    Eight compounds from six Chrysanthemum morifolium cv. 'Hangju' were determined and multivariate statistics, including principle component analysis (PCA) and partial least squares discriminate analysis (PLS-DA) were used to investigate the potential damaging effect of sulfr-fumigating process. Meanwhile, S, Mn, Fe, Cu, Pb were also detected by using ICP-MS and ICP-OES. In this study, dramatic chemical changes were found that the contents of flavonoid aglycones remarkably increased while those of glycosides and hydroxycinnamoylquinic acids were significantly reduced. The PCA score plots showed six samples were clearly classified into the non-fumigated and sulfur-fumigated types. And according to VIP > 1, the most important chemical markers were luteolin, apigenin and luteolin-7-O-glucoside which indicated that the extracted three components might have a marked effect on the discrimination among different group samples. And then, it was found that the residue of sulfur of SHJ were significantly higher than NHJ (P sulfur-fumigated HJ with combining the quantitative chemical analysis and multivariate statistical analysis, and then the result will provide some evidence to evaluat the quality of HJ and control its processing.

  10. Formation of callus and somatic embryos of Cocos nucifera L. cv. Indio Amarillo using semisolid medium

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    Igor Bidot

    2002-07-01

    Full Text Available The objective of this work was evaluated the effect of the concentration of 2,4-D and the type of explant in the formation of callus of Cocos nucifera L. cv. Indio Amarillo. The biggest percentages of explants with callus were obtained with the sections 1 in all the 2,4-D concentration, with a gradual decrease when the section belongs to the most external region of the somatic embryo caulinar zone. The best results in the callus formation process was obtained with the 2,4-D concentrations of 28.0 mg.l-1 and 35.0 mg.l-1, with 90% and 70% of the explants with callus and an average level of development according to the scale of 3.5 and 2.2 at ten weeks of culture. With the objective of to form somatic embryos, the callus obtained with 28.0 mg.l-1 of 2,4-D were put in a culture medium of similar composition to the callus formation medium, but with a lower concentration of 2,4-D (21.0 mg.l-1 and at five weeks of culture was observed as average seven somatic embryos per callus, some of them germinated. Key words: callogenesis, coconut tree, somatic embryogenesis, plant grow regulators

  11. An Efficient Agrobacterium-Mediated Transformation of Strawberry cv. Camarosa by a Dual Plasmid System

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    Fatemeh Haddadi

    2015-02-01

    Full Text Available An Agrobacterium-mediated transformation method was applied to introduce the luciferase reporter gene under the control of the CaMV35S promoter in the pGreen0049 binary vector into strawberry cv. Camarosa. The in vitro regeneration system of strawberry leaves to be used in the transformation was optimized using different TDZ concentrations in MS medium. TDZ at 16 µM showed the highest percentage (100% of shoot formation and the highest mean number of shoots (24 produced per explant. Studies on the effects of different antibiotics, namely timentin, cefotaxime, carbenicillin and ampicillin, on shoot regeneration of strawberry leaf explants showed the best shoot regeneration in the presence of 300 mg/L timentin and 150 mg/L cefotaxime. Assessment of the different factors affecting Agrobacterium mediated-transformation of strawberry with the luciferase gene showed the highest efficiency of putative transformant production (86% in the treatment with no preculture, bacterial OD600 of 0.6 and the addition of 150 mg/L cefotaxime in the pre-selection and selection media. The presence of the luciferase gene in the plant genome was verified by the luciferase reporter gene assay, nested PCR amplification and dot blot of genomic DNA isolated from the young leaves of each putatively transformed plantlet.

  12. Evaluation of oenological potential on clonal selections of cv. Cabernet Sauvignon from Chile

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    Ceppi de Lecco C.

    2014-01-01

    Full Text Available 13 clonal selections of cv Cabernet Sauvignon, established in Nancagua VI Region, Chile (34.39 °S 71.17 °W. The genetic identity of the vines was confirmed by ampelography and microsatellite markers (SSR. Evaluations in the 2012–2013 season include: performance, analytical and sensorial parameters on the wines made by microvinification. The results were statistically analyzed with the Statgraphics Plus program and multiple comparison test of Tukey at 95% confidence level. Sensorially, the wines were evaluated by a panel of 12 experts. The results were likewise analyzed by testing principal components (PCA with covariance matrix without rotation. In the season studied the selection 108 highlighted with a high yield (kg/plant, in the composition of the wine selections generally highlighted for contents of total polyphenols and anthocyanins over average. The results show typical sensory characteristics of wines from that grape variety, and it was possible to group the selections by their attributes (PCA with cherry red wines at different intensities, but without significant differences, with fruity and vegetal aromas, interesting complex flavors and with structured tannins.

  13. HUBUNGAN ANTARA KECERDASAN EMOSIONAL DENGAN ORGANIZATIONAL CITIZENSHIP BEHAVIOR (OCB PADA KARYAWAN CV. ANEKA ILMU SEMARANG

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    Wiwik Sumiyarsih

    2012-04-01

    Full Text Available Karyawan diharapkan untuk menunjukkan perilaku organizational citizenship atau organizational citizenship behavior (OCB, karena OCB dapat meningkatkan efektivitas, produktivitas, dan kesejahteraan organisasi. Penelitian ini bertujuan untuk mengetahui hubungan antara kecerdasan emosional dengan OCB. Subjek penelitian ini adalah 113 karyawan CV. Aneka Ilmu Semarang. Teknik pengambilan sampel dilakukan dengan proporsional random sampling. Metode pengumpulan data menggunakan dua skala, yaitu skala OCB sebanyak 26 item (α = 0,887 dan skala kecerdasan emosional sebanyak 24 item (α = 0.865. Metode analisis menggunakan analisis regresi sederhana dengan perolehan rxy = 0,747 dengan tingkat signifikansi korelasi pada p = 0,001 (p <0,05, yang berarti ada hubungan yang signifikan antara OCB dengan kecerdasan emosional. Tanda positif pada koefisien korelasi menunjukkan arah hubungan positif, yang berarti bahwa semakin tinggi kecerdasan emosional maka semakin tinggi OCB. Kecerdasan emosional memberikan kontribusi sebesar 55,9% dari OCB. Ada faktor lain sebesar 44,1% yang juga berperan namun tidak terungkap dalam penelitian ini. Kata Kunci: kecerdasan emosional, OCB, karyawan

  14. STRUCTURE AND NUTRITIVE VALUE OF DEFERRED PASTURE OF BRACHIARIA DECUMBENS CV. BASILISK DURING THE GRAZING PERIOD

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    Manoel Eduardo Rozalino Santos

    2011-07-01

    Full Text Available The experiment aimed to understand the effect of grazing period on morphology and nutritive value of deferred Brachiaria decumbens cv. Basilisk pasture and on hand-plucking sample with cattle. Subdivided plots were used according to a randomized block design with two replicates. Four grazing periods (1, 31, 57 and 88 days and two forage samples (available in pasture (AP and obtained by hand-plucking (HP were studied. The live leaf laminae (LLL, potentially digestible neutral detergent fiber (PDNDF, potentially digestible dry matter (PDDM and crude protein (CP levels were higher and dead leaf laminae (DLL, and dead stem (DS, neutral detergent fiber (NDF and indigestible NDF percentages were lower in sample of HP in relation to forage AP. The grazing period decreased linearly the LLL, PDNDF, PDDM percentages, as well as increased linearly DS and indigestible NDF levels in forages. The potential selectivity indexes (PSI of LLL and indigestible NDF increased linearly with grazing period. The PSI of live stem was smaller and the PSI of CP was higher in interim periods of grazing. The reduction in deferring period results in B. decumbens with better morphological composition and nutritional value, which favors the animal selectivity.

  15. Perbaikan Pengelolaan Pergudangan Melalui Penerapan Sistem Informasi Pergudangan di CV. ABB

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    Hery Hamdi Azwir

    2017-05-01

    Full Text Available Warehousing management problems that exist in the CV. ABB is the management of warehouses that are susceptible to manipulation of data, methods of work are still very manual such as recording a spare part only handwritten by the warehouse, frequent loss of spare parts in the warehouse, the bon false, and the lack of supervision instruments for oversee the spare parts in the warehouse. To overcome these problems, then made improvements through the implementation of warehouse management information system. Improvements in the form of procurement supervision instruments using the application PHP7 and MySQL, because the existence of the application makes it easy to keep an eye on spare parts in the warehouse, ranging from spare part comes in, spare part out, anyone who requests a part, stock of spare parts of existing in the warehouse, stock outs are used to help finance the manufacture purchase order, reports the spare parts that go into the warehouse of the supplier and request a report part. Not only is it a system of warehousing information can facilitate in making purchase order finance and carry out the approval of the request part of the user. Thus the system becomes more transparent.

  16. Preharvest UV-C radiation influences physiological, biochemical, and transcriptional changes in strawberry cv. Camarosa.

    Science.gov (United States)

    de Oliveira, Isadora Rubin; Crizel, Giseli Rodrigues; Severo, Joseana; Renard, Catherine M G C; Chaves, Fabio Clasen; Rombaldi, Cesar Valmor

    2016-11-01

    Ultraviolet C (UV-C) radiation is known for preventing fungal decay and enhancing phytochemical content in fruit when applied postharvest. However, limited knowledge is available regarding fruit responses to preharvest application of UV-C radiation. Thus, the effects of UV-C radiation on photosynthetic efficiency, dry matter accumulation and partitioning, fruit yield and decay, phytochemical content, and relative transcript accumulation of genes associated with these metabolic pathways were monitored in strawberry (Fragaria x ananassa Duch.) cv. Camarosa. A reduction in photosynthetic efficiency was followed by a decrease in light harvesting complex LhcIIb-1 mRNA accumulation as well as a decrease in yield per plant. Phenylalanine ammonia lyase activity, phenolic, anthocyanin, and L-ascorbic acid contents were higher in UV-C treated fruit. In addition, preharvest UV-C treatment reduced microorganism incidence in the greenhouse and on the fruit surface, increased the accumulation of β-1,3-Gluc and PR-1 mRNA, and prevented fruit decay. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  17. Trehalose 6-phosphate signal is closely related to sorbitol in apple (Malus domestica Borkh. cv. Gala

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    Wen Zhang

    2017-02-01

    Full Text Available Trehalose-6-phosphate (Tre6P is a precursor of trehalose, which is widespread in nature and greatly influences plant growth and development. Tre6P acts as a signal of carbon availability in many plants, but little is known about the function of Tre6P in rosaceous plants, which have specific sorbitol biosynthesis and transportation pathways. In the present study, Tre6P levels and Sorbitol:Tre6P ratios were analyzed in apple (Malus domestica, Borkh. cv. Gala. Tre6P levels were positively correlated with sorbitol content but negatively correlated with sucrose, glucose, and fructose content in developing fruit. However, under sorbitol-limited conditions, Tre6P levels were positively correlated with both sorbitol and sucrose. In the presence of different exogenous sugar supply, Tre6P levels increased corresponding with sorbitol, but this was not the case with sucrose. In addition, Tre6P content and sorbitol:Tre6P ratios were more highly correlated with ADP-glucose levels under sorbitol-limited conditions and fruit development stages, respectively. These results suggest that Tre6P is more closely related to sorbitol than other soluble sugars and has an important role in influencing carbon metabolism in apple.

  18. Agrobacterium-mediated transformation of Vitis Cv. Monastrell suspension-cultured cells: Determination of critical parameters.

    Science.gov (United States)

    Chu, Mingyu; Quiñonero, Carmen; Akdemir, Hülya; Alburquerque, Nuria; Pedreño, María Ángeles; Burgos, Lorenzo

    2016-05-01

    Although some works have explored the transformation of differentiated, embryogenic suspension-cultured cells (SCC) to produce transgenic grapevine plants, to our knowledge this is one of the first reports on the efficient transformation of dedifferentiated Vitis vinifera cv Monastrell SCC. This protocol has been developed using the sonication-assisted Agrobacterium-mediated transformation (SAAT) method. A construct harboring the selectable nptII and the eyfp/IV2 marker genes was used in the study and transformation efficiencies reached over 50 independent transformed SCC per gram of infected cells. Best results were obtained when cells were infected at the exponential phase. A high density plating (500 mg/dish) gave significantly better results. As selective agent, kanamycin was inefficient for the selection of Monastrell transformed SCC since wild type cells were almost insensitive to this antibiotic whereas application of paromomycin resulted in very effective selection. Selected eyfp-expressing microcalli were grown until enough tissue was available to scale up a new transgenic SCC. These transgenic SCC lines were evaluated molecularly and phenotypically demonstrating the presence and integration of both transgenes, the absence of Agrobacterium contamination and the ability of the transformed SCC to grow in highly selective liquid medium. The methodology described here opens the possibility of improving the production of valuable metabolites. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:725-734, 2016. © 2016 American Institute of Chemical Engineers.

  19. Tolerance of mango cv. ´Ataulfo' to irradiation with Co-60 vs. hydrothermal phytosanitary treatment

    Science.gov (United States)

    Gómez-Simuta, Y.; Hernández, Emilio; Aceituno-Medina, Marysol; Liedo, Pablo; Escobar-López, Arseny; Montoya, Pablo; Bravo, Bigail; Hallman, Guy J.; Bustos, M. Emilia; Toledo, Jorge

    2017-10-01

    The use of ionizing irradiation or the use of hot water treatment (HWT) has been demonstrated as a successful commercial phytosanitary treatment during the past two decades. Several countries currently use this technology for commercial treatments to meet plant quarantine requirements. However, hydrothermal treatment has been found to significantly affect the firmness of ;Ataulfo; mango fruit, the susceptibility to damage by cold and it also accelerates their maturation. In this study, we focused on the effect of irradiation doses on the sensorial quality and the physiochemical properties of mango cv ;Ataulfo; compared with the traditional hot water treatment. We found that doses of 150 Gy and 300 Gy of gamma radiation can be applied successfully as well as the hot water treatment. There was no significant difference in between irradiation treatments in terms of weight loss, external and internal color, pH, soluble solids, titratable acidity and firmness, and consumer's acceptance. There was no adverse effect of color appearance, odor and flavor, indicating that consumers will have the willingness to buy and consume irradiated mangoes. Irradiation of mangoes can be a successful post-harvest treatment as an alternative to the hot water treatment.

  20. [In vitro and in vivo effects of mango pulp (Mangifera indica cv. Azucar) in colon carcinogenesis].

    Science.gov (United States)

    Corrales-Bernal, Andrea; Amparo Urango, Luz; Rojano, Benjamín; Maldonado, Maria Elena

    2014-03-01

    Mango pulp contains ascorbic acid, carotenoids, polyphenols, terpenoids and fiber which are healthy and could protect against colon cancer. The aim of this study was to evaluate the antiproliferative and preventive capacity of an aqueous extract of Mangifera indica cv. Azúcar on a human colon adenocarcinoma cell line (SW480) and in a rodent model of colorectal cancer, respectively. The content of total phenolics, flavonoids and carotenoids were also analyzed in the extract. SW480 cell growth was inhibited in a dose and time dependent manner by 22.3% after a 72h exposure to the extract (200 µg/ mL). Colon carcinogenesis was initiated in Balb/c mice by two intra-peritoneal injections of azoxymethane (AOM) at the third and fourth week of giving mango in drinking water (0.3%, 0.6%, 1.25%). After 10 weeks of treatment, in the colon of mice receiving 0.3% mango, aberrant crypt foci formation was inhibited more than 60% (p=0,05) and the inhibition was dose-dependent when compared with controls receiving water. These results show that mango pulp, a natural food, non toxic, part of human being diet, contains bioactive compounds able to reduce growth of tumor cells and to prevent the appearance of precancerous lesions in colon during carcinogenesis initiation.

  1. Optimization of freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel.

    Science.gov (United States)

    Mehrnoush, Amid; Mustafa, Shuhaimi; Yazid, Abdul Manap Mohd

    2012-01-01

    Response surface methodology (RSM) along with central composite design (CCD) was applied to optimize the freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel. The effect of pectinase content (-2.66, 62.66 mg/mL), Arabic gum (-1.21, 10.21%, w/v), and maltodextrin (0.73, 7.26%, w/v) as independent variables on activity, yield, and storage stability of freeze-dried enzyme was evaluated. Storage stability of pectinase was investigated after one week at 4 °C and yield percentage of the enzyme after encapsulation was also determined. The independent variables had the most significant (p mango peel were obtained using 30 mg/mL of pectinase content, 4.5 (%, w/v) of Arabic gum, and 4 (%, w/v) of maltodextrin. Under these conditions, the maximum activity (11.12 U/mL), yield (86.4%) and storage stability (84.2%) of encapsulated pectinase were achieved.

  2. Nitrogen and potassium fertilization in yield and macronutrients contents of heliconia cv. Golden Torch

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    Márkilla Z. Beckmann-Cavalcante

    2016-04-01

    Full Text Available ABSTRACT This study aimed to evaluate the effect of nitrogen (N and potassium (K fertilization in yield and contents of macronutrients in heliconia plants (Heliconia psittacorum x Heliconia spathocircinata Aristeguieta cv. Golden Torch. The experiment was set in a randomized block design, in a 4 x 4 factorial, corresponding to N doses (0, 120, 180 and 240 g of N hole-1 and K doses (0, 120, 180 and 240 g of K2O hole-1 with four replicates and five rhizomes per plot, under field conditions. After one year of cultivation, yield (number of flower stems per square meter and the leaf contents of N, P, K, Ca, Mg and S were determined. The applied N doses did not influence either yield or the leaf contents of the evaluated macronutrients. K fertilization favored the increase in leaf K content and the decrease in the contents of P, Ca and Mg in leaves of heliconias. Under the conditions of this experiment, it is recommended to apply 161.46 g of K2O hole-1 for a maximum yield of 9.89 stems m-2 per month.

  3. Zinc biofortification improves phytochemicals and amino-acidic profile in Brassica oleracea cv. Bronco.

    Science.gov (United States)

    Barrameda-Medina, Yurena; Blasco, Begoña; Lentini, Marco; Esposito, Sergio; Baenas, Nieves; Moreno, Diego A; Ruiz, Juan M

    2017-05-01

    Zn deficiency is currently listed as a major risk factor for human health. Recently, a complimentary solution to mineral malnutrition termed 'biofortification' has been proposed. The aim of this study was to investigate the possible effects of a Zn-biofortification program on Zn levels, amino acidic profile and the phytochemicals content in an edible leafy vegetable, such as Brassica oleracea cv. Bronco. Our results indicate that supplementation of 80-100μM Zn is optimal for maintaining the normal growth of plants and to promote the major Zn concentration in the edible part of B. oleracea. Any further increase of Zn supply induced an accumulation of total amino acids, and increased the enzymatic activities involved in sulfur assimilation and synthesis of phenols, finally resulting in a foliar accumulation of glucosinolates and phenolic compounds. Thus, it could be proposed that the growth of B. oleracea under 80-100μM Zn may increase the intake of this micronutrient and other beneficial compunds for the human health. Copyright © 2017 Elsevier B.V. All rights reserved.

  4. Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex.

    Science.gov (United States)

    Zare, Hamid; Moosavi-Movahedi, Ali Akbar; Salami, Maryam; Mirzaei, Morteza; Saboury, Ali Akbar; Sheibani, Nader

    2013-03-01

    Ficin (EC 3.4.22.3), a cysteine endoproteolytic protease in fig trees' latex, has multiple isoforms. Until now, no data on autolysis of individual ficins (ficin isoforms) are available. Following purification, ficins' autolysis was determined by HPLC chromatogram changes and ultrafiltrations at different temperatures and storage times. These results showed that the number of HPLC peaks in latex proteins purification of Ficus carica cv. Sabz varied from previous fig varieties or cultivars. Proteolytic activity of ficins was inhibited by specific cysteine protease inhibitors, confirming the participation of the cysteine residue in the active site. The zeta potential of the first two eluted peaks (I and II) was negative, while that of other peaks were positive. All ficins were susceptible to autolysis when stored at high temperatures. In contrast, only the last two ficins (B, C) were prone to autolysis at cold temperature after long storage period. The rate of degradation of the ficins was significantly increased with the increased storage time. The ficin (A) related to peak (III) had the highest and the lowest surface hydrophobic patches and ratio of autolytic to proteolytic activity, respectively. Copyright © 2012 Elsevier Ltd. All rights reserved.

  5. The BANANA Project. V. Misaligned and Precessing Stellar Rotation Axes in CV Velorum

    Science.gov (United States)

    Albrecht, Simon; Winn, Joshua N.; Torres, Guillermo; Fabrycky, Daniel C.; Setiawan, Johny; Gillon, Michaël; Jehin, Emmanuel; Triaud, Amaury; Queloz, Didier; Snellen, Ignas; Eggleton, Peter

    2014-04-01

    As part of the Binaries Are Not Always Neatly Aligned project (BANANA), we have found that the eclipsing binary CV Velorum has misaligned rotation axes. Based on our analysis of the Rossiter-McLaughlin effect, we find sky-projected spin-orbit angles of βp = -52° ± 6° and βs = 3° ± 7° for the primary and secondary stars (B2.5V + B2.5V, P = 6.9 days). We combine this information with several measurements of changing projected stellar rotation speeds (vsin i sstarf) over the last 30 yr, leading to a model in which the primary star's obliquity is ≈65°, and its spin axis precesses around the total angular momentum vector with a period of about 140 yr. The geometry of the secondary star is less clear, although a significant obliquity is also implicated by the observed time variations in the vsin i sstarf. By integrating the secular tidal evolution equations backward in time, we find that the system could have evolved from a state of even stronger misalignment similar to DI Herculis, a younger but otherwise comparable binary. Based on observations made with ESOs 2.2 m Telescopes at the La Silla Paranal Observatory under programme ID 084.C-1008 and under MPIA guaranteed time.

  6. Effect of foliar fertilization on Ananas comosus L. Merr. cv. `Cayena lisa' acclimatization

    Directory of Open Access Journals (Sweden)

    Ortelio Hurtado

    2015-07-01

    Full Text Available The low survival and slow growth of in vitro pineapple plants (Ananas comosus L. Merr. in acclimatization stage limit the use of biotechnological techniques for it propagation. The aim of this study was to determine the effect of foliar fertilization in the acclimatization of pineapple plants cv. `Smooth Cayenne'. Two variants of foliar fertilization were compared. The first, plants were fertilized daily after the last irrigation with a minimum dose increased until three months of culture. The second included the same fertilizer at maximum dose with daily dose foliar applications after the last irrigation 10 days from planting to three months of cultivation. As a control, unfertilized plants were included. Every 20 days to three months of culture height (cm of plants was measured, the number of leaves per plant was quantified and the length and width of the leaves was measured. It was observed that fertilization had effect under the experimental conditions tested on the plants variables. After 90 days of culture plants obtained in the treatment with daily fertilization at maximun dose (option 2, met the requirements of height, length and width of the leaf for transplantation to field conditions. Key words: pineapple, propagation, zeolite

  7. Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids.

    Science.gov (United States)

    Kanamoto, Hirosuke; Yamashita, Atsushi; Asao, Hiroshi; Okumura, Satoru; Takase, Hisabumi; Hattori, Masahira; Yokota, Akiho; Tomizawa, Ken-Ichi

    2006-04-01

    Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However, broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene (aadA) under the control of lettuce chloroplast regulatory expression elements, flanked by two adjacent lettuce plastid genome sequences allowing its targeted insertion between the rbcL and accD genes. On average, we obtained 1 transplastomic lettuce plant per bombardment. We show that lettuce leaf chloroplasts can express transgene-encoded GFP to approximately 36% of the total soluble protein. All transplastomic T0 plants were fertile and the T1 progeny uniformly showed stability of the transgene in the chloroplast genome. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants.

  8. Rhizospheric Microflora Escalating Aroma Constituents and Yield Attributes in Ocimum tenuiflorum (L. cv. CIM-Ayu

    Directory of Open Access Journals (Sweden)

    Shilpi Khare Saikia

    2014-01-01

    Full Text Available The exploration of rhizospheric microbial flora for crop yield enhancement is well established. Rhizospheric microbes influence the plant physiology by imparting several beneficial effects, namely, Nitrogen fixation, increased nutrient uptake, and secondary metabolites production on their host plants. The present study investigates the response of Bacillus megaterium ATCC No. 13525, Pseudomonas fluorescens ATCC No. 14581, and Trichoderma viride MTCC No. 167 in alone and combined treatments for their effect on growth and yield parameters in a commercially important Ocimum tenuiflorum L. cv. CIM-Ayu. The plant is therapeutically important for its essential oil constituents, namely, eugenol, β-caryophyllene, and various monoterpenes. The combination treatments, T7 (B. megaterium + P. fluorescens and T8 (B. megaterium + P. fluorescens + T. viride, showed maximum enhancement (27.27% of percentage essential oil as compared to untreated control. Nutrient uptake especially N2 content was significantly increased (43% with the treatment T8 (B. megaterium + P. fluorescens + T. viride. Amongst major essential oil constituents, eugenol content was maximally increased by 58.5% as compared to 42.9% (control indicating a cumulative role of microbial inoculants for crop yield boost-up.

  9. Optimization of Freeze Drying Conditions for Purified Pectinase from Mango (Mangifera indica cv. Chokanan) Peel

    Science.gov (United States)

    Mehrnoush, Amid; Mustafa, Shuhaimi; Yazid, Abdul Manap Mohd

    2012-01-01

    Response surface methodology (RSM) along with central composite design (CCD) was applied to optimize the freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel. The effect of pectinase content (−2.66, 62.66 mg/mL), Arabic gum (−1.21, 10.21%, w/v), and maltodextrin (0.73, 7.26%, w/v) as independent variables on activity, yield, and storage stability of freeze-dried enzyme was evaluated. Storage stability of pectinase was investigated after one week at 4 °C and yield percentage of the enzyme after encapsulation was also determined. The independent variables had the most significant (p < 0.05) effect on pectinase activity and yield of the enzyme. It was observed that the interaction effect of Arabic gum and maltodextrin improved the enzymatic properties of freeze-dried pectinase. The optimal conditions for freeze-dried pectinase from mango peel were obtained using 30 mg/mL of pectinase content, 4.5 (%, w/v) of Arabic gum, and 4 (%, w/v) of maltodextrin. Under these conditions, the maximum activity (11.12 U/mL), yield (86.4%) and storage stability (84.2%) of encapsulated pectinase were achieved. PMID:22489134

  10. Optimization of Freeze Drying Conditions for Purified Pectinase from Mango (Mangifera indica cv. Chokanan Peel

    Directory of Open Access Journals (Sweden)

    Abdul Manap Mohd Yazid

    2012-03-01

    Full Text Available Response surface methodology (RSM along with central composite design (CCD was applied to optimize the freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan peel. The effect of pectinase content (−2.66, 62.66 mg/mL, Arabic gum (−1.21, 10.21%, w/v, and maltodextrin (0.73, 7.26%, w/v as independent variables on activity, yield, and storage stability of freeze-dried enzyme was evaluated. Storage stability of pectinase was investigated after one week at 4 °C and yield percentage of the enzyme after encapsulation was also determined. The independent variables had the most significant (p < 0.05 effect on pectinase activity and yield of the enzyme. It was observed that the interaction effect of Arabic gum and maltodextrin improved the enzymatic properties of freeze-dried pectinase. The optimal conditions for freeze-dried pectinase from mango peel were obtained using 30 mg/mL of pectinase content, 4.5 (%, w/v of Arabic gum, and 4 (%, w/v of maltodextrin. Under these conditions, the maximum activity (11.12 U/mL, yield (86.4% and storage stability (84.2% of encapsulated pectinase were achieved.

  11. Sequence analysis of ORF IV RTBV isolated from tungro infected Oryza sativa L. cv Ciherang

    Science.gov (United States)

    Hastilestari, Bernadetta Rina; Astuti, Dwi; Estiati, Amy; Nugroho, Satya

    2015-09-01

    The Effort to increase rice production is often constrained by pest and disease such as Tungro. The Tungro disease is caused by the joint infection with two dissimilar viruses; a bacil-form-DNA virus, the Rice tungro bacilliform virus(RTBV) and the spherical RNA virus, Rice tungro spherical virus (RTSV) and transmitted by Green leafhopper (Nephotettix virescens). The symptom of disease is caused by the presence of RTBV. The genome of RTBV consists of four Open reading frames (ORFs) which encode functional proteins. Of the four, ORF IV is unique because it exists only in RTBV. The most efficient method of generating disease resistance plants is to look for natural sources of resistance genes in wild or germplasm and then transfer the gene and the accompanying resistance in cultivated crop varieties. The aim of this study is, therefore, to isolate and analyze of 1170 bp gene of ORF 4 of Tungro virus isolated from an Indonesian rice cultivar, Ciherang (Oryza sativa L. cv Indica). DNA sequencing analysis using BLAST showed 94% similarity with the reference sequence gen bank Acc.M65026.1. The comparisons and mutation analysis of DNA sequences were discussed in this research.

  12. Application of OpenCV in Asus Tinker Board for face recognition

    Science.gov (United States)

    Chen, Wei-Yu; Wu, Frank; Hu, Chung-Chiang

    2017-06-01

    The rise of the Internet of Things to promote the development of technology development board, the processor speed of operation and memory capacity increases, more and more applications, can already be completed before the data on the board computing, combined with the network to sort the information after Sent to the cloud for processing, so that the front of the development board is no longer simply retrieve the data device. This study uses Asus Tinker Board to install OpenCV for real-time face recognition and capture of the face, the acquired face to the Microsoft Cognitive Service cloud database for artificial intelligence comparison, to find out what the face now represents the mood. The face of the corresponding person name, and finally, and then through the text of Speech to read the name of the name to complete the identification of the action. This study was developed using the Asus Tinker Board, which uses ARM-based CPUs with high efficiency and low power consumption, plus improvements in memory and hardware performance for the development board.

  13. EFFICIENCY OF RAW MATERIAL INVENTORIES IN IMPROVING SUPPLY CHAIN PERFORMANCE of CV. FIVA FOOD

    Directory of Open Access Journals (Sweden)

    Artadi Nugraha

    2016-01-01

    Full Text Available The production and number of processed food industries have slightly increased; as a result, the companies must compete to maximize their profits by conducting their efficient production process. CV. Fiva Food is one of the companies in the field of processed foods, especially in processed meat that has implemented supply chain management. It is necessary for the company to take measurements of its performance and efficiency for the entire supply chain such as procurement of raw materials. The purposes of this study were to analyze the performance of the company's supply chain and determine the most efficient  method of procurement for its raw materials as well as and to provide recommendations for the company to improve its performance of entire supply chain. This study used SCOR in analyzing the performance of supply chain and EOQ and POQ method to be compared with the method that the company uses to determine which method of procurement for raw materials is the most efficient one. The result showed that based on the matrix, the company's performance is unfavorable when it was compared to the benchmark performance of inventory days of supply. In addition, this study showed that the POQ method produces the lowest total inventory cost with savings of Rp6.647.015 for raw materials of MDM whereas EOQ method produced the lowest total inventory cost with savings of Rp222.153,78 for raw materials of FQ85CL. Keywords: performance suppy chain, SCOR, fiva food, EOQ, POQ

  14. Vitis vinifera L. cv Pinot noir pomace and lees as potential sources of bioactive compounds.

    Science.gov (United States)

    Reis, Gabriel M; Faccin, Henrique; Viana, Carine; Rosa, Marcelo Barcellos da; de Carvalho, Leandro M

    2016-11-01

    Food and agricultural industries generate substantial quantities of phenolic-rich by-products that could be valuable natural sources of antioxidants. The aim of this study was to identify and quantify the phenolic compounds and radical scavenging activities of two by-products (pomace and lees) from Vitis vinifera L. cv Pinot noir. We found a different distribution of phenolic classes (flavanols, flavonols, phenolic acids and stilbenes) and singular scavenging activity against free radicals (hydroxyl, superoxide and peroxyl radicals). The major class of phenolics in pomace was flavanols and in lees was flavonols, with catechin (117.9 ± 2.5 μg g(-1)) and quercetin (42.4 ± 1.2 μg g(-1)) being the most abundant individual compounds. We also found high potential on scavenging activity against superoxide radicals in pomace (80% of scavenging activity) and radical peroxyl (67% scavenging activity). These results show the possibility of using Pinot noir by-products as promising additives or as a source for the development of new products in different segments of the food and cosmetic industries.

  15. Stable MSAP markers for the distinction of Vitis vinifera cv Pinot noir clones.

    Science.gov (United States)

    Ocaña, Juan; Walter, Bernard; Schellenbaum, Paul

    2013-11-01

    Grapevine is one of the most economically important fruit crops. Molecular markers have been used to study grapevine diversity. For instance, simple sequence repeats are a powerful tool for identification of grapevine cultivars, while amplified fragment length polymorphisms have shown their usefulness in intra-varietal diversity studies. Other techniques such as sequence-specific amplified polymorphism are based on the presence of mobile elements in the genome, but their detection lies upon their activity. Relevant attention has been drawn toward epigenetic sources of variation. In this study, a set of Vitis vinifera cv Pinot noir clones were analyzed using the methylation-sensitive amplified polymorphism technique with isoschizomers MspI and HpaII. Nine out of fourteen selective primer combinations were informative and generated two types of polymorphic fragments which were categorized as "stable" and "unstable." In total, 23 stable fragments were detected and they discriminated 92.5 % of the studied clones. Detected stable polymorphisms were either common to several clones, restricted to a few clones or unique to a single clone. The identification of these stable epigenetic markers will be useful in clonal diversity studies. We highlight the relevance of stable epigenetic variation in V. vinifera clones and analyze at which level these markers could be applicable for the development of forthright techniques for clonal distinction.

  16. Plant nutritional status modulates glutamine synthetase levels in ripe tomatoes (Solanum lycopersicum cv. Micro-Tom).

    Science.gov (United States)

    Scarpeci, Telma E; Marro, Martin L; Bortolotti, Santiago; Boggio, Silvana B; Valle, Estela M

    2007-02-01

    Tomato (Solanum lycopersicum) fruit ripening implies that chloroplastic proteins are degraded and new proteins are synthesized. Supplementary nutrition is frequently required when tomato plants begin to fruit and continues until the end of the plant's life cycle. Ammonium assimilation is crucial in these fruit maturation and ripening processes. Glutamine synthetase (GS; EC 6.3.1.2), the main ammonium-fixing enzyme in plants, could not be detected in red fruits of several tomato varieties when growing under standard nutrition. In this paper, we analyze the influence of the nutritional status on the ammonium assimilation capacity of ripe tomato (cv. Micro-Tom) fruit. For this purpose, GS expression and protein profiles were followed in mature green and red fruits harvested from plants grown under standard or supplemented nutrition. Under standard nutrient regime (weekly supplied with 0.5 x Hoagland solution) GS activity was found in chloroplasts (GS2) of mature green fruits, but it was not detected either in the chromoplasts or in the cytosol of red fruits. When plants were shifted to a supplemented nutritional regime (daily supplied with 0.5 x Hoagland solution), GS was found in red fruits. Also, cytosolic transcripts (gs1) preferentially accumulated in red fruits under high nutrition. These results indicate that mature green Micro-Tom fruits assimilate ammonia through GS2 under standard nutrition, while ripe red fruits accumulate GS1 under high nutrition, probably in order to assimilate the extra N-compounds made available through supplemented nutrition.

  17. Nutritional disorder of lettuce cv. Veronica in nutrient solution with suppressed macronutrients

    Directory of Open Access Journals (Sweden)

    Thiago Batista Firmato de Almeida

    2011-06-01

    Full Text Available Lettuce is the most popular of the leafy vegetables. It is known worldwide and its consumption occurs mainly in the natural form. The objective was to evaluate the effect of macronutrient omission on the growth and nutritional status of the lettuce cv. Veronica, and to describe the visual symptoms of nutritional deficiency. The treatments complete consisted of the solution and the individual omission of N, P, K, Ca, Mg and S, under a completely randomized design with three replications. Plants were grown in Hoagland & Arnon, in pots (8L. At 56 days after transplant we evaluated plant height, leaf number, leaf area, SPAD index, dry matter of shoots, roots and whole plant, levels of macronutrients in shoots and roots, and the nature of nutritional disorders. The omission of nutrients affected the growth variables. The nutrients found in lettuce plants from nutrient solution and the complete omission in the shoot were, respectively, N= 23.2 to 9.5, P= 5.4 to 1.3, K= 58,9 to 3.2, Ca= 12.1 to 3.6, Mg= 5.5 to 0.7 and S= 3.2 to 1.5g.kg-1. The omission of macronutrients caused quality losses, since it affected the nutrition of the vegetables and this resulted in morphological changes, reflected as symptoms of deficiency for each nutrient.

  18. Effect of postharvest hot-water and heat treatment on quality of date palm (cv. Stamaran

    Directory of Open Access Journals (Sweden)

    I. Hazbavi

    2015-06-01

    Full Text Available In this research, fresh harvested date palm (Phoenix dactylifera L. cv. “Stamaran” was treated with hot water rinsing and dried (HWR at 50 °C (HWR-50, 60 °C (HWR-60 and 70 °C (HWR-70. The effect of these heat treatments on fruit quality was investigated during 6 months at ambient temperature storage (25 °C of temperature and 75% of humidity. Moisture, pH, color, weight loss, Brix and firmness of the samples were studied. Results indicated that during storage the moisture content and color changed significantly. The major change was observed for firmness where a maximum force for puncture test varied from about 3.5 to 2 N forces for all samples after 6 months of storage at 25 °C. Harvesting at Tamr stage followed by treating the fruits with hot water, drying and storing at 25 °C (Especially HWR-70 showed to be a promising method for maintaining date palm fruit storage quality.

  19. Embryogenic callus formation in Dioscorea rotundata Poir cv. `Blanco de Guinea'

    Directory of Open Access Journals (Sweden)

    Dayana Rodríguez González

    2014-07-01

    Full Text Available Yam contributes to energy and nutritional requirements of most of the population of developing countries. However, their extensive culture is constrained by the limited availability of planting material with physiological and sanitary quality, and also part of the harvesting is used as seed in the next planting. For this reason, it is necessary to establish a methodology for plant regeneration and somatic embryogenesis could facilitate their micropropagation and genetic improvement. This study aimed to form embryogenic callus in Dioscorea rotundata Poir cv. `White Guinea'. The effect of the addition of 2,4-dichlorophenoxyacetic acid (2,4-D (0, 1.0, 2.0 and 4.0 mg l-1 was determined, in combination with three types of explants from in vitro plants (leaves petiole, petiole segments and root sections. The highest percentage of embryogenic callus was obtained with 1.0 mg l-1 2,4-D and leaves with petiole as explants. These were characterized by the presence of compact whitish nodules. Key words: 2,4-dichlorophenoxyacetic acid, somatic embryogenesis, micropropagation, yam

  20. Perianth bottom-specific blue color development in Tulip cv. Murasakizuisho requires ferric ions.

    Science.gov (United States)

    Shoji, Kazuaki; Miki, Naoko; Nakajima, Noriyuki; Momonoi, Kazumi; Kato, Chiharu; Yoshida, Kumi

    2007-02-01

    The entire flower of Tulipa gesneriana cv. Murasakizuisho is purple, except the bottom, which is blue. To elucidate the mechanism of the different color development in the same petal, we prepared protoplasts from the purple and blue epidermal regions and measured the flavonoid composition by HPLC, the vacuolar pH by a proton-selective microelectrode, and element contents by the inductively coupled plasma (ICP) method. Chemical analyses revealed that the anthocyanin and flavonol compositions in both purple and blue colored protoplasts were the same; delphinidin 3-O-rutinoside (1) and major three flavonol glycosides, manghaslin (2), rutin (3) and mauritianin (4). The vacuolar pH values of the purple and blue protoplasts were 5.5 and 5.6, respectively, without any significant difference. However, the Fe(3+) content in the blue protoplast was approximately 9.5 mM, which was 25 times higher than that in the purple protoplasts. We could reproduce the purple solution by mixing 1 with two equimolar concentrations of flavonol with lambda(vismax) = 539 nm, which was identical to that of the purple protoplasts. Furthermore, addition of Fe(3+) to the mixture of 1-4 gave the blue solution with lambda(vismax) = 615 nm identical to that of the blue protoplasts. We have established that Fe(3+) is essential for blue color development in the tulip.

  1. Alkaloids from Narcissus poeticus cv. Pink Parasol of various structural types and their biological activity.

    Science.gov (United States)

    Šafratová, Marcela; Hošťálková, Anna; Hulcová, Daniela; Breiterová, Kateřina; Hrabcová, Veronika; Machado, Marta; Fontinha, Diana; Prudêncio, Miguel; Kuneš, Jiří; Chlebek, Jakub; Jun, Daniel; Hrabinová, Martina; Nováková, Lucie; Havelek, Radim; Seifrtová, Martina; Opletal, Lubomír; Cahlíková, Lucie

    2018-02-01

    Fifteen Amaryllidaceae alkaloids (1-15) of various structural types were isolated by standard chromatographic methods from fresh bulbs of Narcissus poeticus cv. Pink Parasol. The chemical structures were elucidated by MS, and 1D and 2D NMR spectroscopic analyses, and by comparison with literature data. Narcipavline (5) and narcikachnine (6) are reported here for the first time. In their structure are combined two basic structural types of Amaryllidaceae alkaloids (galanthamine- and galanthindole-structural types), which represent a new structural type of these compounds. Alkaloids isolated in sufficient amounts were evaluated for their human erythrocytic acetylcholinesterase, and human serum butyrylcholinesterase (HuBuChE) inhibition activity using Ellman's method. Z-Gly-Pro-p-nitroanilide was used as substrate in the prolyl oligopeptidase (POP) assay. Untested alkaloids were also screened for their cytotoxic activity against a small panel of human cancer cells, which spanned cell lines from different tissue types. In parallel, MRC-5 human fibroblasts were employed to determine overall toxicity against noncancerous cells. Some compounds were evaluated for their antiprotozoal activity. The newly isolated alkaloid narcipavline (5) showed interesting HuBuChE inhibition activity (IC 50  = 24.4 ± 1.2 µM), and norlycoramine (11) demonstrated promising POP inhibition (IC 50  = 0.21 ± 0.01 mM).

  2. Do sawfish Pristis spp. represent South Africa's first local extirpation ...

    African Journals Online (AJOL)

    Do sawfish Pristis spp. represent South Africa's first local extirpation of marine elasmobranchs in the modern era? ... African Journal of Marine Science ... This paper traces the decline in sawfish catches from 1951 and assesses the current population status and local extinction risk, based on historical and current records up ...

  3. Molecular characterization of Azotobacter spp. nifH gene Isolated ...

    African Journals Online (AJOL)

    The nifH gene sequence of the nitrogen-fixing bacterium Azotobacter spp. was determined with the use of polymerase chain reaction (PCR). The Azotobacter species was isolated from marine source in two different seasons. They were cultivated under laboratory conditions using Nitrogen free Azotobacter specific medium.

  4. Motility patterns of filamentous sulfur bacteria, Beggiatoa spp

    DEFF Research Database (Denmark)

    Dunker, Rita; Røy, Hans; Kamp, Anja

    2011-01-01

    The large sulfur bacteria, Beggiatoa spp., live on the oxidation of sulfide with oxygen or nitrate, but avoid high concentrations of both sulfide and oxygen. As gliding filaments, they rely on reversals in the gliding direction to find their preferred environment, the oxygen–sulfide interface.We ...... Beggiatoa accumulate high nitrate concentrations in internal vacuoles as an alternative electron acceptor to oxygen....

  5. Asymptomatic presence of Nosema spp. in Spanish commercial apiaries.

    Science.gov (United States)

    Fernández, José Manuel; Puerta, Francisco; Cousinou, Mercedes; Dios-Palomares, Rafaela; Campano, Francisco; Redondo, Laura

    2012-10-01

    Nosemosis is caused by intracellular parasites (Nosema apis and Nosema ceranae) that infect the midgut epithelial cells in adult honey bees. Recent studies relate N. ceranae to Colony Collapse Disorder and there is some suggestion that Nosema spp., especially N. ceranae, induces high mortality in honey bees, a fact that is considered as a serious threat for colony survival. 604 samples of adult honey bees for Nosema spp. analysis were collected from beekeeping colonies across Spain and were analysed using PCR with capillary electrophoresis. We also monitored 77 Andalusian apiaries for 2 years; the sampled hives were standard healthy colonies, without any special disease symptoms. We found 100% presence of Nosema spp. in some locations, indicating that this parasite was widespread throughout the country. The two year monitoring indicated that 87% of the hives with Nosema spp. remained viable, with normal honey production and biological development during this period of time. The results of these trials indicated that both N. ceranae and N. apis could be present in these beehives without causing disease symptom and that there is no evidence for the replacement of N. apis by N. ceranae, supporting the hypothesis that nosemosis is not the main reason of the collapse and death of beehives. Copyright © 2012 Elsevier Inc. All rights reserved.

  6. Remarks concerning two sympatric seedeaters Poliospiza spp. in ...

    African Journals Online (AJOL)

    Turner, D.A., Finch, B.F, & Hunter, N.D. Remarks concerning the all-black coastal boubous. (Laniarius spp.) of Kenya and southern Somalia. Bulletin of the British ... northern striatipectus bears little or no similarity to the southern African nominate reichardi, itself largely endemic to the miombo woodlands of Zambia and ...

  7. Prevalence and antibiotics susceptibility profile of Enterococcus spp ...

    African Journals Online (AJOL)

    This study investigated the prevalence and antibiotics susceptibility of Enterococcus spp. isolated from patients and some selected hospital environment in Abuja, Nigeria. The samples included clinical and environmental. The clinical samples included stool, urine and wound swabs while the environmental samples ...

  8. 21 CFR 866.3270 - Flavobacterium spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Flavobacterium spp. serological reagents. 866.3270 Section 866.3270 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... disease caused by bacteria belonging to the genus Flavobacterium and provides epidemiological information...

  9. 21 CFR 866.3140 - Corynebacterium spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Corynebacterium spp. serological reagents. 866.3140 Section 866.3140 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN... belonging to the genus Corynebacterium and provides epidemiological information on diseases caused by these...

  10. 21 CFR 866.3600 - Schistosoma spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Schistosoma spp. serological reagents. 866.3600 Section 866.3600 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... genus Schistosoma. Schistosomiasis is characterized by a variety of acute and chronic infections. Acute...

  11. 21 CFR 866.3065 - Bordetella spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Bordetella spp. serological reagents. 866.3065 Section 866.3065 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... identification aids in the diagnosis of diseases caused by bacteria belonging to the genus Bordetella and...

  12. 21 CFR 866.3660 - Shigella spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Shigella spp. serological reagents. 866.3660 Section 866.3660 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... the diagnosis of shigellosis caused by bacteria belonging to the genus Shigella and provides...

  13. 21 CFR 866.3085 - Brucella spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Brucella spp. serological reagents. 866.3085 Section 866.3085 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES...) caused by bacteria belonging to the genus Brucella and provides epidemiological information on diseases...

  14. 21 CFR 866.3355 - Listeria spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Listeria spp. serological reagents. 866.3355 Section 866.3355 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... belonging to the genus Listeria, and provides epidemiological information on diseases caused by these...

  15. 21 CFR 866.3200 - Echinococcus spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Echinococcus spp. serological reagents. 866.3200 Section 866.3200 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... tapeworms belonging to the genus Echinococcus and provides epidemiological information on this disease...

  16. 21 CFR 866.3630 - Serratia spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Serratia spp. serological reagents. 866.3630 Section 866.3630 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... identification aids in the diagnosis of disease caused by bacteria belonging to the genus Serratia and provides...

  17. 21 CFR 866.3340 - Klebsiella spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Klebsiella spp. serological reagents. 866.3340 Section 866.3340 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... belonging to the genus Klebsiella and provides epidemiological information on these diseases. These...

  18. 21 CFR 866.3040 - Aspergillus spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Aspergillus spp. serological reagents. 866.3040 Section 866.3040 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... the genus Aspergillus. Aspergillosis is a disease marked by inflammatory granulomatous (tumor-like...

  19. 21 CFR 866.3125 - Citrobacter spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Citrobacter spp. serological reagents. 866.3125 Section 866.3125 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... bacteria belonging to the genus Citrobacter and provides epidemiological information on diseases caused by...

  20. 21 CFR 866.3300 - Haemophilus spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Haemophilus spp. serological reagents. 866.3300 Section 866.3300 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... belonging to the genus Haemophilus and provides epidemiological information on diseases cause by these...

  1. 21 CFR 866.3375 - Mycoplasma spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Mycoplasma spp. serological reagents. 866.3375 Section 866.3375 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES.... The identification aids in the diagnosis of disease caused by bacteria belonging to the genus...

  2. 21 CFR 866.3870 - Trypanosoma spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Trypanosoma spp. serological reagents. 866.3870 Section 866.3870 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... protozoans belonging to the genus Trypanosoma. Trypanosomiasis in adults is a chronic disease characterized...

  3. 21 CFR 866.3035 - Arizona spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Arizona spp. serological reagents. 866.3035 Section 866.3035 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES.... The identification aids in the diagnosis of disease caused by bacteria belonging to the genus Arizona...

  4. Abalone Haliotis spp. are in considerable demand worldwide ...

    African Journals Online (AJOL)

    spamer

    Abalone Haliotis spp. are in considerable demand worldwide, especially in the Far East. This has en- couraged industries that involve not only harvesting natural populations but also, as such populations have declined, setting up artificial cultures of appropriate species (Lo-Chai Chen 1990, Britz 1996, Knauer et al. 1996).

  5. Genetic diversity analysis of mustard ( Brassica spp.) germplasm ...

    African Journals Online (AJOL)

    Molecular characterization of 16 mustard (Brassica spp.) genotypes by using 12 RAPD markers revealed that three primers GLA-11, OPB-04 and OPD-02 showed good technical resolution and sufficient variations among different genotypes. A total of 40 RAPD bands were scored of which 38 (94.87%) polymorphic ...

  6. Response of Soybean to Inoculation with Bradyrhizobium spp. in ...

    African Journals Online (AJOL)

    Soybean [Glycine max (L.) Merrill] is an important crop in Ethiopia. However, its productivity is constrained by a number of factors among which soil salinity is one the major problems. Therefore, field and greenhouse experiments were conducted to examine the effectiveness of exotic and locally isolated Bradyrhizobium spp.

  7. Investigation of tick-borne bacteria (Rickettsia spp., Anaplasma spp., Ehrlichia spp. and Borrelia spp.) in ticks collected from Andean tapirs, cattle and vegetation from a protected area in Ecuador.

    Science.gov (United States)

    Pesquera, Cristina; Portillo, Aránzazu; Palomar, Ana M; Oteo, José A

    2015-01-24

    Ixodid ticks play an important role in the transmission and ecology of infectious diseases. Information about the circulation of tick-borne bacteria in ticks is lacking in Ecuador. Our aims were to investigate the tick species that parasitize Andean tapirs and cattle, and those present in the vegetation from the buffer zone of the Antisana Ecological Reserve and Cayambe-Coca National Park (Ecuador), and to investigate the presence of tick-borne bacteria. Tick species were identified based on morphologic and genetic criteria. Detection of tick-borne bacteria belonging to Rickettsia, Anaplasma, Ehrlichia and Borrelia genera was performed by PCRs. Our ticks included 91 Amblyomma multipunctum, 4 Amblyomma spp., 60 Rhipicephalus microplus, 5 Ixodes spp. and 1 Ixodes boliviensis. A potential Candidatus Rickettsia species closest to Rickettsia monacensis and Rickettsia tamurae (designated Rickettsia sp. 12G1) was detected in 3 R. microplus (3/57, 5.3%). In addition, Anaplasma spp., assigned at least to Anaplasma phagocytophilum (or closely related genotypes) and Anaplasma marginale, were found in 2 A. multipunctum (2/87, 2.3%) and 13 R. microplus (13/57, 22.8%). This is the first description of Rickettsia sp. in ticks from Ecuador, and the analyses of sequences suggest the presence of a potential novel Rickettsia species. Ecuadorian ticks from Andear tapirs, cattle and vegetation belonging to Amblyomma and Rhipicephalus genera were infected with Anaplasmataceae. Ehrlichia spp. and Borrelia burgdorferi sensu lato were not found in any ticks.

  8. Trichoderma spp. from rhizosphere soil and their antagonism ...

    African Journals Online (AJOL)

    One hundred and forty six (146) isolates of Trichoderma spp. were obtained from rhizosphere soils of potato plants in the middle areas of Gansu Province, China. By means of dual culture method, they were examined for antagonism against Fusarium sambucinum, which causes potato dry rot. Ten of the isolates were found ...

  9. Prevalence and antibiogram of Shigella and Salmonella spp. from ...

    African Journals Online (AJOL)

    Diarrheal diseases remain the major cause of morbidity and mortality in children under five years of age. Salmonella and Shigella species are among the leading causes of diarrhea in children. The aim of this study was to determine the prevalence and antimicrobial profiles of Salmonella and Shigella spp. in children less ...

  10. Seroprevalence of Neospora spp. in horses from Central Province of ...

    African Journals Online (AJOL)

    HP

    2013-02-27

    Feb 27, 2013 ... Vet. Parasitol. 79:269-274. Hoane JS, Gennari SM, Dubey JP, Ribeiro MG, Borges AS Yai, LE,. Aguiar DM, Cavalcante GT, Bonesi GL, Howe DK (2006). Prevalence of Sarcocystis neurona and Neospora spp. infection in horses from. Brazil based on presence of serum antibodies to parasite surface antigen.

  11. Molecular detection of Brucella spp. from broth culture of clinical ...

    African Journals Online (AJOL)

    PCR was employed to detect Brucella spp. from broth cultures of clinical samples using a group specific primer based on IS6501 sequence. The sensitivity and specificity of this assay was confirmed by Southern hybridization analysis using a digoxigenin-labeled DNA probe while reproducibility of the analysis was ...

  12. Salmonella spp. dynamics in wild blueberry, Vaccinium angustifolium Aiton

    Science.gov (United States)

    A six-year field study was conducted in the two major wild, or lowbush, blueberry growing regions in Maine, Midcoast and Downeast. This study used data from two cropping cycles (four years) to model the dynamics of Salmonella spp. prevalence in wild blueberry fields (Vaccinium angustifolium Aiton). ...

  13. The isoprenoid-precursor dependence of Plasmodium spp

    NARCIS (Netherlands)

    van der Meer, Jan-Ytzen; Hirsch, Anna K. H.

    2012-01-01

    Due to the increase in resistance of Plasmodium spp. against available antimalarials, there is a need for new, effective and innovative drugs. The non-mevalonate pathway for the biosynthesis of the universal isoprenoid precursors, which is absent in humans, is suggested as an attractive source of

  14. Anti-inflammatory of both Eucalyptus spp. and Pistascia lentiscus ...

    African Journals Online (AJOL)

    Background: Eucalyptus spp. and Pistascia lentiscus are among the Palestinian trees that are traditionally used in folkloric medicine in treating many diseases; leaves of which are thought to have anti-inflammatory, antibacterial and antioxidant effects. The goal of this study is to evaluate the in vitro inhibitory effect of ...

  15. Aeromonas spp. in het Nederlandse drinkwater; een orienterend onderzoek

    NARCIS (Netherlands)

    Versteegh JFM; During M; Havelaar AH; Koot W

    1986-01-01

    In dit rapport worden uitvoering en resultaten van een orienterend onderzoek naar het voorkomen van Aeromonas spp. in het Nederlandse drinkwater in 1985 besproken. Het bleek dat in 71% van de onderzochte monsters (rein water "af pompstation" en water uit het distributienet) in 100 ml

  16. Host Responses to Malassezia spp. in the Mammalian Skin

    Directory of Open Access Journals (Sweden)

    Florian Sparber

    2017-11-01

    Full Text Available The skin of mammalian organisms is home for a myriad of microbes. Many of these commensals are thought to have beneficial effects on the host by critically contributing to immune homeostasis. Consequently, dysbiosis can have detrimental effects for the host that may manifest with inflammatory diseases at the barrier tissue. Besides bacteria, fungi make an important contribution to the microbiota and among these, the yeast Malassezia widely dominates in most areas of the skin in healthy individuals. There is accumulating evidence that Malassezia spp. are involved in a variety of skin disorders in humans ranging from non- or mildly inflammatory conditions such as dandruff and pityriasis versicolor to more severe inflammatory skin diseases like seborrheic eczema and atopic dermatitis. In addition, Malassezia is strongly linked to the development of dermatitis and otitis externa in dogs. However, the association of Malassezia spp. with such diseases remains poorly characterized. Until now, studies on the fungus–host interaction remain sparse and they are mostly limited to experiments with isolated host cells in vitro. They suggest a multifaceted crosstalk of Malassezia spp. with the skin by direct activation of the host via conserved pattern recognition receptors and indirectly via the release of fungus-derived metabolites that can modulate the function of hematopoietic and/or non-hematopoietic cells in the barrier tissue. In this review, we discuss our current understanding of the host response to Malassezia spp. in the mammalian skin.

  17. Host Responses to Malassezia spp. in the Mammalian Skin

    Science.gov (United States)

    Sparber, Florian; LeibundGut-Landmann, Salomé

    2017-01-01

    The skin of mammalian organisms is home for a myriad of microbes. Many of these commensals are thought to have beneficial effects on the host by critically contributing to immune homeostasis. Consequently, dysbiosis can have detrimental effects for the host that may manifest with inflammatory diseases at the barrier tissue. Besides bacteria, fungi make an important contribution to the microbiota and among these, the yeast Malassezia widely dominates in most areas of the skin in healthy individuals. There is accumulating evidence that Malassezia spp. are involved in a variety of skin disorders in humans ranging from non- or mildly inflammatory conditions such as dandruff and pityriasis versicolor to more severe inflammatory skin diseases like seborrheic eczema and atopic dermatitis. In addition, Malassezia is strongly linked to the development of dermatitis and otitis externa in dogs. However, the association of Malassezia spp. with such diseases remains poorly characterized. Until now, studies on the fungus–host interaction remain sparse and they are mostly limited to experiments with isolated host cells in vitro. They suggest a multifaceted crosstalk of Malassezia spp. with the skin by direct activation of the host via conserved pattern recognition receptors and indirectly via the release of fungus-derived metabolites that can modulate the function of hematopoietic and/or non-hematopoietic cells in the barrier tissue. In this review, we discuss our current understanding of the host response to Malassezia spp. in the mammalian skin. PMID:29213272

  18. Occurrence of Trichinella spp . in wild animals in northwestern Libya ...

    African Journals Online (AJOL)

    The present study determined the occurrence of Trichinella spp. in captured and some perished wildlife animals which included 70 hedgehogs, 19 red foxes, 13 common jackals and 8 crested porcupines in northwestern Libya. Muscle samples of these animals were examined by trichinoscopy. Trichinella larvae were ...

  19. Transformation Artisanale Des Crevettes ( Penaeus Spp) Au Sud Du ...

    African Journals Online (AJOL)

    Transformation Artisanale Des Crevettes ( Penaeus Spp) Au Sud Du Bénin : Évaluation Des Performances Techniques Des Équipements Et Procédés De Fumage. ... This study aims to evaluate and compare the technical performances of the main important traditional smoking processes in the south of Benin. Therefore ...

  20. Antifungals susceptibility pattern of Candida spp . isolated from ...

    African Journals Online (AJOL)

    The highest susceptibility of the isolates was seen for nystatin 62 (83.78%), ketoconazole 61 (82.43%) and fluconazole 60 (81.08%). Conclusion: Despite the noticeable resistance of Candida spp. isolates to miconazole and itraconazole, the results indicate that nystatin, ketoconazole and fluconazole are the drugs of choice ...