WorldWideScience

Sample records for sporulation takes place

  1. Taking place, screening place

    DEFF Research Database (Denmark)

    Hansen, Kim Toft; Waade, Anne Marit

    2019-01-01

    We introduce location studies as a new empirical approach to screen studies. Location studies represent an interdisciplinary perspective, including media, aesthetics and geography, and reflect a growing interest in places in a global media and consumption culture. The chapter analyses two recent......) with one being traditional and the other being commercial; both dramas include discussions of localities and social heritage, and both use local sports as a common metaphor for social cohesion; and both series have been partly funded by a local film Danish commissioner. However, The Legacy is shot...... to a large extent in studios, while Norskov is shot entirely on location. The study is based on interviews with producers, broadcasters, location scouts, production designers and writers, as well as quantitative and qualitative textual analyses of television drama series, the geographical places, and related...

  2. Is channeling of fission tracks taking place?

    CERN Document Server

    Yada, K

    1999-01-01

    A single crystal of natural zircon which is sliced to have (010) basal plane and thinned by ion thinning is electron microscopically observed after slow neutron irradiation to ascertain whether channeling of the nuclear fission fragments is taking place or not. A fairly large number of the induced fission tracks are recognized at low magnification images where a considerable number of them are parallel to low-index lattice planes such as 100, 001, 101, 301, 103 though their directions changed some time up to several degrees. High resolution images of fission tracks often show a variety of zigzag passing of the tracks along low-index lattice planes in atomistic level. The rate of the tracks which are parallel to these low-index lattice planes is fairly high as about 45%, which strongly suggests that channeling of the fission tracks is taking place.

  3. Mathematical modeling of phase interaction taking place in materials processing

    International Nuclear Information System (INIS)

    Zinigrad, M.

    2002-01-01

    The quality of metallic products depends on their composition and structure. The composition and the structure are determined by various physico-chemical and technological factors. One of the most important and complicated problems in the modern industry is to obtain materials with required composition, structure and properties. For example, deep refining is a difficult task by itself, but the problem of obtaining the material with the required specific level of refining is much more complicated. It will take a lot of time and will require a lot of expanses to solve this problem empirically and the result will be far from the optimal solution. The most effective way to solve such problems is to carry out research in two parallel direction. Comprehensive analysis of thermodynamics, kinetics and mechanisms of the processes taking place at solid-liquid-gaseous phase interface and building of the clear well-based physico-chemical model of the above processes taking into account their interaction. Development of mathematical models of the specific technologies which would allow to optimize technological processes and to ensure obtaining of the required properties of the products by choosing the optimal composition of the raw materials. We apply the above unique methods. We developed unique methods of mathematical modeling of phase interaction at high temperatures. These methods allows us to build models taking into account: thermodynamic characteristics of the processes, influence of the initial composition and temperature on the equilibrium state of the reactions, kinetics of homogeneous and heterogeneous processes, influence of the temperature, composition, speed of the gas flows, hydrodynamic and thermal factors on the velocity of the chemical and diffusion processes. The models can be implemented in optimization of various metallurgical processes in manufacturing of steels and non-ferrous alloys as well as in materials refining, alloying with special additives

  4. 49 CFR 40.221 - Where does an alcohol test take place?

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Where does an alcohol test take place? 40.221... WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Testing Sites, Forms, Equipment and Supplies Used in Alcohol Testing § 40.221 Where does an alcohol test take place? (a) A DOT alcohol test must take place at an...

  5. Key European Grid event to take place in Geneva

    CERN Multimedia

    2006-01-01

    EGEE'06 is the main conference of the EGEE project, which is co-funded by the European Union and hosted by CERN. More than 90 partners all over Europe and beyond are working together in EGEE to provide researchers in both academia and industry with access to major computing resources, independent of their geographic location. The largest user community of the EGEE Grid is the High-Energy Physics community and in particular the LHC experiments, which are already making heavy use of the infrastructure to prepare for data taking. However, with the many new challenges faced by EGEE in its second phase that started in April this year, an even broader audience than at previous EGEE conferences is expected. In particular, a large number of related Grid projects will feature prominently in both plenary and parallel sessions during the 5 days of this event. Industry will also be well represented, highlighting the EGEE project's commitment to technology transfer to industry. CERN is the host of the conference, which i...

  6. Pirated Siderophores Promote Sporulation in Bacillus subtilis.

    Science.gov (United States)

    Grandchamp, Gabrielle M; Caro, Lews; Shank, Elizabeth A

    2017-05-15

    In microbial communities, bacteria chemically and physically interact with one another. Some of these interactions are mediated by secreted specialized metabolites that act as either intraspecies or interspecies signals to alter gene expression and to change cell physiology. Bacillus subtilis is a well-characterized soil microbe that can differentiate into multiple cell types, including metabolically dormant endospores. We were interested in identifying microbial interactions that affected sporulation in B. subtilis Using a fluorescent transcriptional reporter, we observed that coculturing B. subtilis with Escherichia coli promoted sporulation gene expression via a secreted metabolite. To identify the active compound, we screened the E. coli Keio Collection and identified the sporulation-accelerating cue as the siderophore enterobactin. B. subtilis has multiple iron acquisition systems that are used to take up the B. subtilis- produced siderophore bacillibactin, as well as to pirate exogenous siderophores such as enterobactin. While B. subtilis uses a single substrate binding protein (FeuA) to take up both bacillibactin and enterobactin, we discovered that it requires two distinct genes to sporulate in response to these siderophores (the esterase gene besA for bacillibactin and a putative esterase gene, ybbA , for enterobactin). In addition, we found that siderophores from a variety of other microbial species also promote sporulation in B. subtilis Our results thus demonstrate that siderophores can act not only as bacterial iron acquisition systems but also as interspecies cues that alter cellular development and accelerate sporulation in B. subtilis IMPORTANCE While much is known about the genetic regulation of Bacillus subtilis sporulation, little is understood about how other bacteria influence this process. This work describes an interaction between Escherichia coli and B. subtilis that accelerates sporulation in B. subtilis The interaction is mediated by the E

  7. Sporulation of Cyclospora sp. oocysts.

    OpenAIRE

    Smith, H V; Paton, C A; Mitambo, M M; Girdwood, R W

    1997-01-01

    Cyclospora sp. oocysts sporulated maximally at 22 and 30 degrees C for 14 days retarded sporulation. Up to 12% of human- and baboon-derived oocysts previously stored at 4 degrees C for 1 to 2 months sporulated when stored for 6 to 7 days at 30 degrees C.

  8. The Poetic Word in the Taking-Place of Language: Aesthetics, Ethics, and Politics

    Directory of Open Access Journals (Sweden)

    Maria Rosa Duarte de Oliveira

    2016-09-01

    Full Text Available This work aims to investigate the nature and function of the poetic word from its relation with aesthetics, ethics, and politics. We are stirred to know the ways poetic language, inscribed within language, may appear as a gesture of resistance and subversion. This is due to its investments both in non-representation and in the presentation of an (almost rising being in a hic et nunc of its presence in the "taking place" of language. Contemporary philosophers, such as Alain Badiou and Giorgio Agamben, suggest possible answers to this question through the operation of negativity that takes place in language so as to make it uninformative. Barthes also seems to follow the same path by lingering on the emptiness of the appearance of "this is" in the poetic form of haiku, which prevents any further interpretation. We expect that these ways of thinking the poetic may offer literary criticism new investigative parameters.

  9. Waste Disposal: Processes Taking Place (on the way) from the Repository to the Biosphere

    International Nuclear Information System (INIS)

    Put, M.

    2000-01-01

    The main objective of SCK-CEN's R and D programme on the processes taking place on the way from the repository to the biosphere is to provide reliable and defensible models and parameters on the migration of dissolved radionuclides and gases through the host formation (Boom Clay) and the backfill materials of a deep geological repository for high level radioactive waste. The programme and main achievements in this topical area in 1999 are summarised

  10. DETERMINING THE FEATURES OF SPORTSWEAR TAKING PLACE IN FAST FASHION COLLECTIONS

    Directory of Open Access Journals (Sweden)

    Birsen ÇİLEROĞLU

    2014-07-01

    Full Text Available Spor ts occupies the first place among most siginificant factors increasing quality of life . It has b ecome difficult to allocate proper time for sports in the course of heavy work pace and flow of life . Such circumstances have led people to increase minor sport activities which could be done during short times allocated from daily living, thus, orienting people‟s clothing preference towards sportswear . The feeling of easiness and comfort sportswear offer to individuals enhances further such preference . The feeling of comfort individuals feel in their clothing depends on the presence of physiologic and psy chologic coherence between their bodies and environment . Demand for sportswear allowing easy - movement increased upon rise in life dynamism and standards, it began to be preferred regarding comfort of use and to take its place in daily clothing, too, define d as “casual” clothing . Spor tswear being preferred very much ; has caused the firms making and producing fashion and clothing design to give place in their collections to sportswear category . Particularly, in firms where model and clothing varieties are pl enty and new model design is made in short intervals, named as, “fast fashion” , tendency towards sportswear is growing increasingly . The sale rates of sportswear, utilization rates of which are growing increasingly, has maximum value among total clothing s ales in E - business field, too. In this research, it has been aimed to determine the features of sportswear taking place in “fast fashion” clothing collections . In order to accomplish this aim, 2014 collections of four different brands taking place in natio nal and international markets have been examined through visual analysis method . In the examinations; sportswear styles of the brand, model and style differences between brands and states of using 2014 fashion trends have been taken into account. The data obtained at the end of the analyses made have been

  11. Questions and Answers Regarding Actions to Take When Ending Shelter-in-Place

    Energy Technology Data Exchange (ETDEWEB)

    Shumpert, B.

    2003-12-30

    Shelter-in-place has found increasing acceptance as an effective protective action option for communities participating in the Chemical Stockpile Emergency Preparedness Program. Studies have confirmed that it can provide optimum protection under certain accident conditions. However, emergency managers and planners, as well as the public, continue to be troubled by the need to end sheltering when the plume has passed in order to avoid sustained exposure to the small amount of agent that has penetrated the shelter. One of the concerns posed by this necessity is uncertainty regarding what hazards will then be faced in the environment outside the shelter and what actions can be taken to avoid those hazards. This report attempts to address those uncertainties. It recognizes that there is an extremely low probability that the environment outside the shelter will be contaminated with chemical agent residue. However, as people comply with an official recommendation to leave their shelters, they probably can't be certain that the environment is free from contamination. Therefore, this report identifies and explains specific and simple actions they can take to avoid the possibility of exposure to chemical agent hazards outside their shelters. It addresses such issues as the actions people should take upon ending shelter-in-place, what clothing they should wear, how they should handle animals, and what they should do about food in their homes and produce in their gardens.

  12. [Marketing approval and market surveillance of medical devices in Germany: Where does policy integration take place?].

    Science.gov (United States)

    Lang, Achim

    2014-01-01

    Since 2011 new regulatory measures regarding medical devices have been set up with the aim to eliminate obstacles to innovations and to find more coordinated ways to marketing authorisation and market surveillance. This essay investigates whether these new and existing coordination mechanisms build up to a Joined-up Government approach. The analysis shows that the regulatory process should be adjusted along several dimensions. First, many organisations lack awareness regarding their stakeholders and focus solely on their immediate organisational activities. Second, the regulatory process (marketing authorisation and market surveillance) is too fragmented for an effective communication to take place. Finally, the underlying strategy process is an ad-hoc approach lacking continuity and continued involvement of, in particular, the responsible federal ministries. Copyright © 2013. Published by Elsevier GmbH.

  13. RELIGIOUS TRANSFORMATION OF SENI DODOD TAKING PLACE AT MEKAR WANGI VILLAGE SOUTH BANTEN

    Directory of Open Access Journals (Sweden)

    Yuliawan Kasmahidayat

    2012-11-01

    Full Text Available Religious transformation of Seni Dodod taking place at Mekar Wangi Village, SaketiDistrict, Pandeglang Regency, South Banten, Banten Province enriches the analysis of thecommunity cultural pattern based on the art characteristics born and developing in thecommunity. In relation to that, this study describes the cultural phenomenon of religioustransformation as the ideological basis of the pre-modern, modern and postmodern communities.Multidisciplinary qualitative method was employed in this study and the data needed werecollected by in-depth interview, participatory observation and documentation techniques. Thefindings show that the Seni Dodod has the belief or the religion adhered to by the people living atMekar Wangi Village as its religious background. Such a belief affects the relation patternbetween the individuals and their community, nature and God. This is used as the reference inwhat is done in the agricultural process, making Seni Dodod a cult and realizatrion. When thecultural transformation process was taking place, it was found that there was a point of contactbetween traditional arts and modern arts outside the domain of Seni Dodod . Nowadays newcompositions of Seni Dodod have been created functioning as instruments of wedding andkhitanan rituals (khitanan = a feast celebrating a circumcision. The process of acculturation wasformally performed at school by using its compositions as the learning material of art andculture. Its application in its original form and composition was informally inherited throughSeni Dodod studios, the youth and villagers. Its religious meaningfulness was reflected from thecountry life performed by the people living at Mekar Wangi village. Such meaningfulness wasbased on that given by the leaders of pondok pesantren (Muslem boarding school and on theexplanation clarifying, showing, separating and elaborating seven clauses of Al-Qur’an. Themeaningfulness provided resulted in intactness of movements, the

  14. Makro- and micromorphological evidence of processes taking place during Albeluvisol development in S Norway

    Science.gov (United States)

    Sauer, Daniela; Schülli-Maurer, Isabelle; Sperstad, Ragnhild; Sørensen, Rolf

    2014-05-01

    We studied two soil chronosequences in S Norway to identify processes involved in Albeluvisol formation. For this purpose, field observation of vertical and horizontal sections of soil profiles, soil chemical and mineralogical analyses were carried out, and in particular, micromorphological analysis was applied. The study area is located at the western and eastern side of the Oslofjord, S Norway, in the counties Vestfold and Østfold. This region is characterized by continuous glacio-isostatic uplift over the entire Holocene. Hence, the age of the land surface continuously increases from the coast towards higher elevations. Twelve soil profiles in loamy marine sediments were studied. Based on macro- and micromorphological observations and analytical data progressive soil formation is characterized as follows: As soon as the land surface is raised above sea level, five major processes are initiated: 1) development of deep desiccation cracks, forming a polygonal pattern; 2) compaction, taking place as soon as the coarse pores have been drained; 3) pyrite oxidation and release of sulfuric acid; 4) carbonate dissolution by acids from pyrite and iron oxidation resulting in rapid decarbonatization of the originally calcareous sediments; 5) precipitation of iron hypocoatings and coatings in the capillary fringe Soon after these very early processes have taken place, limited water permeability of the fine-textured sediments leads to horizon differentiation into Ah, Eg and Btg horizons within less than 2.1 ka. Eg horizons become lighter in colour with time. Also illuvial clay is already observed in the 2.1 ka-old soil. Soil pH in the upper part of the E horizon of this soil is already too low for significant clay mobilization. Clay illuviation is still active in all soils studied, but the upper boundary of the zone where pH favours clay mobilization is at 20-50 cm depth. Progressive clay illuviation over time is recorded in increasing thickness of clay coatings and

  15. What it Takes to Successfully Implement Technology for Aging in Place: Focus Groups With Stakeholders.

    Science.gov (United States)

    Peek, Sebastiaan Theodorus Michaël; Wouters, Eveline J M; Luijkx, Katrien G; Vrijhoef, Hubertus J M

    2016-05-03

    There is a growing interest in empowering older adults to age in place by deploying various types of technology (ie, eHealth, ambient assisted living technology, smart home technology, and gerontechnology). However, initiatives aimed at implementing these technologies are complicated by the fact that multiple stakeholder groups are involved. Goals and motives of stakeholders may not always be transparent or aligned, yet research on convergent and divergent positions of stakeholders is scarce. To provide insight into the positions of stakeholder groups involved in the implementation of technology for aging in place by answering the following questions: What kind of technology do stakeholders see as relevant? What do stakeholders aim to achieve by implementing technology? What is needed to achieve successful implementations? Mono-disciplinary focus groups were conducted with participants (n=29) representing five groups of stakeholders: older adults (6/29, 21%), care professionals (7/29, 24%), managers within home care or social work organizations (5/29, 17%), technology designers and suppliers (6/29, 21%), and policy makers (5/29, 17%). Transcripts were analyzed using thematic analysis. Stakeholders considered 26 different types of technologies to be relevant for enabling independent living. Only 6 out of 26 (23%) types of technology were mentioned by all stakeholder groups. Care professionals mentioned fewer different types of technology than other groups. All stakeholder groups felt that the implementation of technology for aging in place can be considered a success when (1) older adults' needs and wishes are prioritized during development and deployment of the technology, (2) the technology is accepted by older adults, (3) the technology provides benefits to older adults, and (4) favorable prerequisites for the use of technology by older adults exist. While stakeholders seemed to have identical aims, several underlying differences emerged, for example, with regard

  16. Model of Wagons’ Placing-In and Taking-Out Problem in a Railway Station and Its Heuristic Algorithm

    Directory of Open Access Journals (Sweden)

    Chuijiang Guo

    2014-01-01

    Full Text Available Placing-in and taking-out wagons timely can decrease wagons’ dwell time in railway stations, improve the efficiency of railway transportation, and reduce the cost of goods transportation. We took the locomotive running times between goods operation sites as weights, so the wagons’ placing-in and taking-out problem could be regarded as a single machine scheduling problem, 1pijCmax, which could be transformed into the shortest circle problem in a Hamilton graph whose relaxation problem was an assignment problem. We used a Hungarian algorithm to calculate the optimal solution of the assignment problem. Then we applied a broken circle and connection method, whose computational complexity was O(n2, to find the available satisfactory order of wagons’ placing-in and taking-out. Complex problems, such as placing-in and transferring combined, taking-out and transferring combined, placing-in and taking-out combined, or placing-in, transferring, and taking-out combined, could also be resolved with the extended algorithm. A representative instance was given to illustrate the reliability and efficiency of our results.

  17. OECD Global Science Forum's Astronomy Workshop to take place in Munich

    Science.gov (United States)

    2003-11-01

    members of the national astronomical community. The International Astronomical Union (IAU) and the European Southern Observatory (ESO) are explicitly represented. Experts from the world-wide astronomy community have been invited to set the stage and provide input for the discussions. The choice by Germany and the OECD to make Munich the venue of this Global Science Forum Workshop is no coincidence. It is a recognition of the important role played by many institutions in the Munich region in the field of Astronomy and Astrophysics. They include the Ludwig-Maximilians-Universität where the Workshop will take place, the Max-Planck-Institut für Astrophysik, the Max-Planck Institut für Extraterrestrische Physik and the European Southern Observatory. These institutions are all participating in large programmes and projects in astronomy. ESO, for its part, is at the leading edge of world astronomy with its flagship facility, the Very Large Telescope in Paranal (Chile) and the newly started ALMA project at Chajnantor (Chile), being carried out in partnership between Europe and North America. Public Talks (Munich) on December 1, 2003 As a prelude to the Workshop, two public keynote presentations will take place on December 1 at the Deutsches Museum in Munich at 18:00 CET. The speakers are Malcolm Longair, Jacksonian Professor of Natural Philosophy and Head of Laboratory, Cavendish Laboratory, Cambridge (UK) and Martin Harwit, Professor Emeritus of Astronomy, Cornell University, and former Director of the National Air and Space Museum, Washington, DC (USA). The talks will be given in English and the entry to this public event is free. Professor Longair will speak on "Astrophysics and Cosmology in the Twenty-First Century" and Professor Harwit will speak on "The Growth of Understanding of our Universe". You can find more informaton on the Public Talks web page.

  18. Protein patterns of yeast during sporulation

    International Nuclear Information System (INIS)

    Litske Petersen, J.G.; Kielland-Brandt, M.C.; Nilsson-Tillgren, T.

    1979-01-01

    High resolution two-dimensional gel electrophoresis was used to study protein synthesis during synchronous meiosis and ascospore formation of Saccharomyces cerevisiae. The stained protein patterns of samples harvested at any stage between meiotic prophase and the four-spore stage in two sporulating strains showed the same approximately 250 polypeptides. Of these only a few seemed to increase or decrease in concentration during sporulation. The characteristic pattern of sporulating yeast was identical to the pattern of glucose-grown staitonary yeast cells adapted to respiration. The latter type of cells readily initiates meiosis when transferred to sporulation medium. This pattern differed from the protein patterns of exponentially growing cells in glucose or acetate presporulation medium. Five major proteins in stationary and sporulating yeast cells were not detected in either type of exponential culture. Two-dimensional autoradiograms of [ 35 S]methionine-labelled yeast proteins revealed that some proteins were preferentially labelled during sporulation, while other proteins were labelled at later stages. These patterns differed from the auroradiograms of exponentially growing yeast cells in glucose presporulation medium in a number of spots. No differences were observed when stained gels or autoradiograms of sporulating cultures and non-sporulating strains in sporulation medium were compared. (author)

  19. Taking back place-names – from dusty library to digital life

    DEFF Research Database (Denmark)

    Knudsen, Bo Nissen

    mobility of the book format into a digital context – by making the content available as an application for mobile devices such as smart phones and iPads? Adding geocodes to the name articles could open up the possibility of a digital place-name lexicon allowing the end user to move around in a place......Danish place-names have been under publication since 1922 in the scientific series Danmarks Stednavne (Place-Names of Denmark) but only recently the huge project of a digitization of the series has been undertaken. Around 120,000 name articles are now on their way to the web as part of the Digital...... atlas of the Danish historical-administrative geography. Digitization and presentation of a scientific place-names edition poses many interesting problems in itself, especially regarding the variation over time in both the selection of names and the build-up of scholarly knowledge. How are we to convey...

  20. DSC studies of retrogradation and amylose-lipid transition taking place in gamma-irradiated wheat starch

    International Nuclear Information System (INIS)

    Ciesla, K.; Gluszewski, W.; Eliasson, A.C.

    2006-01-01

    It has been already shown that degradation resulting from gamma irradiation induces a decrease in order of starch granules and influences gelatinisation taking place during heating of starch and flour suspensions. In presented paper, DSC (differential scanning calorimetry) studies were carried out for wheat starch, non-irradiated and irradiated using doses in the range from 5 to 30 kGy. The influence of the conditions applied during DSC measurements on the possibility to observe differences between the amylose-lipid complex transition and retrogradation taking place in the non-irradiated and particularly irradiated starch samples was checked. The better differentiation between the amylose-lipid complex transition taking place in particular samples accompanied by the better reproducity were obtained in the case of dense suspensions as compared to the watery suspensions as well as during the first analysis performed for the recrystallised gels

  1. An Experimental Investigation of the Process of Isotope Exchange that Takes Place when Heavy Water Is Exposed to the Atmosphere

    Science.gov (United States)

    Deeney, F. A.; O'Leary, J. P.

    2009-01-01

    We have used the recently developed method for rapid measurement of maximum density temperature to determine the rate at which hydrogen and deuterium isotope exchange takes place when a sample of heavy water is exposed to the atmosphere. We also provide a simple explanation for the observed linear rate of transition. (Contains 2 figures.)

  2. Advice from Rural Elders: What It Takes to Age in Place

    Science.gov (United States)

    Dye, Cheryl J.; Willoughby, Deborah F.; Battisto, Dina G.

    2011-01-01

    Older adults prefer to age in place (AIP), and there are psychological, physiological, and economic benefits in doing so. However, it is especially challenging to AIP in rural communities. AIP models have been tested in urban settings and age-segregated communities, but they are not appropriate for rural communities. This paper presents rural AIP…

  3. DETERMINING THE FEATURES OF SPORTSWEAR TAKING PLACE IN FAST FASHION COLLECTIONS

    OpenAIRE

    ÇİLEROĞLU, Birsen; KIVILCIMLAR, İnci

    2015-01-01

    Sports occupies the first place among most siginificant factors increasing quality of life. It has become difficult to allocate proper time for sports in the course of heavy work pace and flow of life. Such circumstances have led people to increase minor sport activities which could be done during short times allocated from daily living, thus, orienting people’s clothing preference towards sportswear. The feeling of easiness and comfort sportswear offer to individuals enhances further such pr...

  4. The albedo problem in the case of multiple synthetic scattering taking place in a plane-symmetric slab

    International Nuclear Information System (INIS)

    Shafiq, A.; Meyer, H.E. de; Grosjean, C.C.

    1985-01-01

    An approximate model based on an improved diffusion-type theory is established for treating multiple synthetic scattering in a homogeneous slab of finite thickness. As in the case of the exact treatment given in the preceding paper (Part I), it appears possible to transform the considered transport problem into an equivalent fictitious one involving multiple isotropic scattering, therefore permitting the application of an established corrected diffusion theory for treating isotropic scattering taking place in a convex homogeneous medium bounded by a vacuum in the presence of various types of sources. The approximate values of the reflection and transmission coefficients are compared with the rigorous values listed in Part I. In this way, the high accuracy of the approximation is clearly demonstrated. (author)

  5. DSC Studies of Retrogradation and Amylose-Lipid Complex Transition Taking Place in Gamma Irradiated Wheat Starch

    International Nuclear Information System (INIS)

    Ciesla, K.

    2006-01-01

    Degradation resulting from gamma irradiation induces decrease in order of starch granules and influences the processes occurring in starch-water system. Differential scanning calorimetry (DSC) was applied at present for studying the effect of radiation with doses of 5 - 30 kGy on amylose-lipid complex transition and retrogradation occurring in wheat starch gels. Influence of the conditions applied during DSC measurements and intermediate storage was tested on the possibility to observe radiation effect. Wheat starch was irradiated with 60 C o gamma rays in a gamma cell Issledovatiel placed in the Department of Radiation Chemistry, INCT. DSC measurements were performed for ca. 50% and ca. 20% gels during heating - cooling - heating cycles (up to 3 cycles) in the temperature range 10 - 150 degree at heating and cooling rates of 10, 5 and 2.5 degree min - 1. The Seiko DSC 6200 calorimeter was used. Decrease in amylose-lipid complex transition temperature was found already after irradiation of wheat starch with a dose of 5 kGy showing modificatin of the complex structure. The differences between the irradiated and the non-irradiated samples became the easier seen in the every foregoing heating or cooling cycle as compared to the preceeding one. It is because that thermal treatment causes decrease of transition temperature in all the irradiated samples, with no effect or increase of that temperature observed in the non-irradiated ones. Irradiation hinders retrogradation taking place in ca. 50% gels but facilitates retrogradation occurring in ca. 20 % gels. Moreover, the expanded differences between the amylose-lipid complex formed in the irradiated and non-irradiated gels result due to their recrystallisation. Storage of the gels induces decrease in the temperature of the complex transition as compared to the last cycle of the first analysis. That decrease was, however, more significant in the case of all the irradiated samples than in the case of the initial sample. In

  6. Where and How Do Aging Processes Take Place in Everyday Life? Answers From a New Materialist Perspective

    Directory of Open Access Journals (Sweden)

    Grit Höppner

    2018-04-01

    Full Text Available In the last decade, the focus of studies on age and aging has fundamentally changed from biological to symbolic, discursive, and cultural phenomena. Currently, the most studied topic in material gerontology is the materiality of age and aging in the context of everyday life. Scholars in this area have thus been making an important contribution to a material understanding of aging processes. As we understand them, however, both social constructivist and material gerontological concepts reach their limit when it comes to the questions of where and how aging processes actually take place in everyday life. In order to answer these two questions, we review social constructivist ideas with a particular focus on the “doing age” concept and material gerontological assumptions regarding human subjects, their material environments, and their relations. We then suggest rethinking bodily limitations and agencies addressed by scholars in the field of new materialism. The aim is to develop a new materialist-inspired understanding of aging processes that helps to reconstruct the material-discursive co-production of aging processes. These processes are deployed as mutual entanglements of materiality and meaning as well as of humans and non-human agency. This approach emphasizes the decentralization of the human actor and thus helps to map the material-discursive complexity of aging processes as relational co-products of humans and non-humans in everyday life.

  7. What does it take to make them stay? : how place satisfaction relates to willingness to stay of the creative class

    OpenAIRE

    Annell, Jonas; Terman, Felix

    2017-01-01

    Swedish students in smaller university host cities leave in favor of more attractive places after having completed their studies. Failing to retain newly graduated students is a problem for university host cities as educated people are associated with the group referred to as the creative class. Members of the creative class are drivers of regional economic growth, and have historically proven to be important for creating and developing the well-being of cities. Different place attributes hav...

  8. Modeling sporulation of Fusicladium carpophilum on nectarine twig lesions: relative humidity and temperature effects.

    Science.gov (United States)

    Lalancette, N; McFarland, K A; Burnett, A L

    2012-04-01

    The production of conidia by Fusicladium carpophilum on twig lesions was quantitatively modeled as a function of temperature and duration of high relative humidity. During peak sporulation periods in 2007, 2008, and 2009, 1-year-old twigs bearing abundant overwintering lesions were removed from a heavily infected 'Redgold' nectarine orchard, placed in trays at high relative humidity (>95%), and incubated at eight constant temperatures for seven durations, resulting in a factorial design of 56 treatment combinations. Conidia numbers were estimated with a hemacytometer. Results from a six-stage modeling process indicated that, at any given temperature, spore production during high relative humidity periods increased in a monomolecular- to Gompertz-like pattern. The Richards model, with shape parameters of 0.79 to 0.90, was found to provide the best overall fit. When the asymptote and rate parameters were derived as functions of temperature using Gaussian and quadratic models, respectively, the duration of high relative humidity and temperature described 90 to 94% of the variation in conidia production. Predictions of the final models were highly correlated with observed levels of sporulation (r > 0.94; P < 0.0001), indicating an excellent fit to the data. The optimum temperature for sporulation, based on fitting a Gaussian model to the maximum sporulation levels at each temperature, was 17.9 to 20.2°C, with an overall average of 18.8°C. The derived models give a quantitative description of sporulation by F. carpophilum and may have potential use in simulators and forecasting systems.

  9. Effects of sporulation pH on the heat resistance and the sporulation of Bacillus cereus.

    Science.gov (United States)

    Mazas, M; López, M; González, I; Bernardo, A; Martín, R

    1997-11-01

    Spores of Bacillus cereus ATCC 7004, 4342 and 9818 were obtained in nutrient agar at several pH from 5.9 to 8.3. The optimum pH for sporulation was around 7, but good production of spores was obtained in the range 6.5-8.3. With all three strains, D-values clearly dropped with sporulation pH, decreasing by about 65% per pH unit. z-Values were not significantly modified (P > 0.05) by this factor. Mean z-values of 7.13 degrees C +/- 0.16 for strain 7004, 7.67 degrees C +/- 0.04 for 4342 and 8.80 degrees C +/- 0.64 for 9818 were obtained.

  10. Sporulation and Germination of Paenibacillus larvae Cells.

    Science.gov (United States)

    Mahdi, Osama S; Fisher, Nathan A

    2018-02-22

    Endospores are metabolically dormant cells formed by a variety of Gram-positive bacteria within the phylum Firmicutes in response to nutrient limiting or otherwise unfavorable growth conditions. American foulbrood disease (AFB) is a serious disease of honeybees that is caused by the introduction of Paenibacillus larvae endospores into a honeybee colony. Progression to fulminant disease and eventual collapse of the colony requires multiple rounds of endospore germination, vegetative replication, endospore formation, and subsequent spread within the colony. This unit includes protocols for the in vitro sporulation and germination of P. larvae to assist investigators in the study of these processes. © 2018 by John Wiley & Sons, Inc. Copyright © 2018 John Wiley & Sons, Inc.

  11. Stuck between a ROC and a hard place? Barriers to the take up of green energy in the UK

    International Nuclear Information System (INIS)

    Diaz-Rainey, Ivan; Ashton, John K.

    2008-01-01

    This paper examines the UK mechanisms for ensuring future investment in renewable energy through consumer adoption of green energy tariffs and the Renewable Obligation Certificate (ROC) system. Using a national survey and focus groups the stated willingness by UK customers to pay a premium for renewable or green energy and actual take up of such tariffs is assessed. Substantial differences between willingness to pay for and the adoption of green energy tariffs are reported. This disparity is linked to a range of factors including consumer confusion, lack of supply, complexities of constructing 'green source' tariffs under the ROC system and a lack of customer trust. It is concluded that the re-definition of the green energy market in favour of 'green source' tariffs, greater direct compliance with the Renewable Obligation by addressing supply constraints, and efforts in providing clearer information and choices for consumers via a compulsory green energy accreditation scheme are required if willing consumers' are to contribute to investment in renewable energy

  12. The Investigation of Mobbing Events Taking Place at Higher Education Institutions in Turkey Considering the Reflections on Media

    Directory of Open Access Journals (Sweden)

    Çubukçu Zühal

    2015-08-01

    considering that they are published nationally, reference newspapers and belong to different publication groups. The data used in this study was taken from the archive of the three newspapers. When the archives of the newspapers was scanned, the word “mobbing” was taken as key word for the search. In qualitative researches, newspapers are considered to be public archive and used as reference documents. The data obtained was grouped depending on the newspapers which it was taken from, on the year of publication, on where they took place, on the gender of the victims and on who the sufferers are.

  13. Sporulation in soil as an overwinter survival strategy in Saccharomyces cerevisiae.

    Science.gov (United States)

    Knight, Sarah J; Goddard, Matthew R

    2016-02-01

    Due to its commercial value and status as a research model there is an extensive body of knowledge concerning Saccharomyces cerevisiae's cell biology and genetics. Investigations into S. cerevisiae's ecology are comparatively lacking, and are mostly focused on the behaviour of this species in high sugar, fruit-based environments; however, fruit is ephemeral, and presumably, S. cerevisiae has evolved a strategy to survive when this niche is not available. Among other places, S. cerevisiae has been isolated from soil which, in contrast to fruit, is a permanent habitat. We hypothesize that S. cerevisiae employs a life history strategy targeted at self-preservation rather than growth outside of the fruit niche, and resides in forest niches, such as soil, in a dormant and resistant sporulated state, returning to fruit via vectors such as insects. One crucial aspect of this hypothesis is that S. cerevisiae must be able to sporulate in the 'forest' environment. Here, we provide the first evidence for a natural environment (soil) where S. cerevisiae sporulates. While there are further aspects of this hypothesis that require experimental verification, this is the first step towards an inclusive understanding of the more cryptic aspects of S. cerevisiae's ecology. © FEMS 2015.

  14. How Quorum Sensing Connects Sporulation to Necrotrophism in Bacillus thuringiensis.

    Directory of Open Access Journals (Sweden)

    Stéphane Perchat

    2016-08-01

    Full Text Available Bacteria use quorum sensing to coordinate adaptation properties, cell fate or commitment to sporulation. The infectious cycle of Bacillus thuringiensis in the insect host is a powerful model to investigate the role of quorum sensing in natural conditions. It is tuned by communication systems regulators belonging to the RNPP family and directly regulated by re-internalized signaling peptides. One such RNPP regulator, NprR, acts in the presence of its cognate signaling peptide NprX as a transcription factor, regulating a set of genes involved in the survival of these bacteria in the insect cadaver. Here, we demonstrate that, in the absence of NprX and independently of its transcriptional activator function, NprR negatively controls sporulation. NprR inhibits expression of Spo0A-regulated genes by preventing the KinA-dependent phosphorylation of the phosphotransferase Spo0F, thus delaying initiation of the sporulation process. This NprR function displays striking similarities with the Rap proteins, which also belong to the RNPP family, but are devoid of DNA-binding domain and indirectly control gene expression via protein-protein interactions in Bacilli. Conservation of the Rap residues directly interacting with Spo0F further suggests a common inhibition of the sporulation phosphorelay. The crystal structure of apo NprR confirms that NprR displays a highly flexible Rap-like structure. We propose a molecular regulatory mechanism in which key residues of the bifunctional regulator NprR are directly and alternatively involved in its two functions. NprX binding switches NprR from a dimeric inhibitor of sporulation to a tetrameric transcriptional activator involved in the necrotrophic lifestyle of B. thuringiensis. NprR thus tightly coordinates sporulation and necrotrophism, ensuring survival and dissemination of the bacteria during host infection.

  15. Effect of saccharides on growth, sporulation rate and δ-endotoxin ...

    African Journals Online (AJOL)

    sucrose, lactose and maltose) as carbon sources on growth and sporulation rate of Bacillus thuringiensis MPK13 as well as δ-endotoxin production was investigated using 400 ml shake flasks culture. Substantially high growth and sporulation ...

  16. Effects of whey on the colonization and sporulation of arbuscular ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-05-18

    May 18, 2009 ... and positive in some applications (G.i + W50, G.i + W100), but the effects of soil pH, saltiness and CaCO3 content on both the colonization and sporulation are statistically insignificant. Key words: Arbuscular mycorrhizal fungus (AMF), whey, colonization, spore density. INTRODUCTION. The building and ...

  17. Effects of temperature on the germination, sporulation, and in - vivo ...

    African Journals Online (AJOL)

    This research was conducted to investigate the effect of temperature and on the germination, sporulation and in vivo infection of Sphaerotheca fuliginea on healthy water melon. The experiment was laid out in completely Randomized Design (CRD) in the laboratory with 5 replications in each case. Mycelium growth was ...

  18. When and Where Learning is Taking Place: Multisynaptic Changes in Strength During Different Behaviors Related to the Acquisition of an Operant Conditioning Task by Behaving Rats.

    Science.gov (United States)

    Fernández-Lamo, Iván; Delgado-García, José M; Gruart, Agnès

    2018-03-01

    Although it is generally assumed that brain circuits are modified by new experiences, the question of which changes in synaptic efficacy take place in cortical and subcortical circuits across the learning process remains unanswered. Rats were trained in the acquisition of an operant conditioning in a Skinner box provided with light beams to detect animals' approaches to lever and feeder. Behaviors such as pressing the lever, eating, exploring, and grooming were also recorded. Animals were chronically implanted with stimulating and recording electrodes in hippocampal, prefrontal, and subcortical sites relevant to the task. Field synaptic potentials were evoked during the performance of the above-mentioned behaviors and before, during, and after the acquisition process. Afferent pathways to the hippocampus and the intrinsic hippocampal circuit were slightly modified in synaptic strength during the performance of those behaviors. In contrast, afferent and efferent circuits of the medial prefrontal cortex were significantly modified in synaptic strength across training sessions, mostly at the moment of the largest change in the learning curve. Performance of behaviors nondirectly related to the acquisition process (exploring, grooming) also evoked changes in synaptic strength across training. This study helps to understand when and where learning is being engraved in the brain. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  19. Model-based definition of population heterogeneity and its effects on metabolism in sporulating Bacillus subtilis.

    Science.gov (United States)

    Morohashi, Mineo; Ohashi, Yoshiaki; Tani, Saeka; Ishii, Kotaro; Itaya, Mitsuhiro; Nanamiya, Hideaki; Kawamura, Fujio; Tomita, Masaru; Soga, Tomoyoshi

    2007-08-01

    The soil bacterium Bacillus subtilis forms dormant, robust spores as a tactic to ensure survival under conditions of starvation. However, the sporulating culture includes sporulating and non-sporulating cells, because a portion of the cell population initiates sporulation in wild-type strain. We anticipated that the population effect must be considered carefully to analyse samples yielding population heterogeneity. We first built a mathematical model and simulated for signal transduction of the sporulation cue to see what mechanisms are responsible for generating the heterogeneity. The simulated results were confirmed experimentally, where heterogeneity is primarily modulated by negative feedback circuits, resulting in generation of a bistable response within the sporulating culture. We also confirmed that mutants relevant to negative feedback yield either sporulating or non-sporulating subpopulations. To see the effect of molecular mechanism between sporulating and non-sporulating cells in distinct manner, metabolome analysis was conducted using the above mutants. The metabolic profiles exhibited distinct characteristics with time regardless of whether sporulation was initiated or not. In addition, several distinct characteristics of metabolites were observed between strains, which was inconsistent with previously reported data. The results imply that careful consideration must be made in the interpretation of data obtained from cells yielding population heterogeneity.

  20. Avoidance of plants unsuitable for the symbiotic fungus in leaf-cutting ants: Learning can take place entirely at the colony dump.

    Directory of Open Access Journals (Sweden)

    Andrés Arenas

    Full Text Available Plants initially accepted by foraging leaf-cutting ants are later avoided if they prove unsuitable for their symbiotic fungus. Plant avoidance is mediated by the waste produced in the fungus garden soon after the incorporation of the unsuitable leaves, as foragers can learn plant odors and cues from the damaged fungus that are both present in the recently produced waste particles. We asked whether avoidance learning of plants unsuitable for the symbiotic fungus can take place entirely at the colony dump. In order to investigate whether cues available in the waste chamber induce plant avoidance in naïve subcolonies, we exchanged the waste produced by subcolonies fed either fungicide-treated privet leaves or untreated leaves and measured the acceptance of untreated privet leaves before and after the exchange of waste. Second, we evaluated whether foragers could perceive the avoidance cues directly at the dump by quantifying the visits of labeled foragers to the waste chamber. Finally, we asked whether foragers learn to specifically avoid untreated leaves of a plant after a confinement over 3 hours in the dump of subcolonies that were previously fed fungicide-treated leaves of that species. After the exchange of the waste chambers, workers from subcolonies that had access to waste from fungicide-treated privet leaves learned to avoid that plant. One-third of the labeled foragers visited the dump. Furthermore, naïve foragers learned to avoid a specific, previously unsuitable plant if exposed solely to cues of the dump during confinement. We suggest that cues at the dump enable foragers to predict the unsuitable effects of plants even if they had never been experienced in the fungus garden.

  1. Avoidance of plants unsuitable for the symbiotic fungus in leaf-cutting ants: Learning can take place entirely at the colony dump.

    Science.gov (United States)

    Arenas, Andrés; Roces, Flavio

    2017-01-01

    Plants initially accepted by foraging leaf-cutting ants are later avoided if they prove unsuitable for their symbiotic fungus. Plant avoidance is mediated by the waste produced in the fungus garden soon after the incorporation of the unsuitable leaves, as foragers can learn plant odors and cues from the damaged fungus that are both present in the recently produced waste particles. We asked whether avoidance learning of plants unsuitable for the symbiotic fungus can take place entirely at the colony dump. In order to investigate whether cues available in the waste chamber induce plant avoidance in naïve subcolonies, we exchanged the waste produced by subcolonies fed either fungicide-treated privet leaves or untreated leaves and measured the acceptance of untreated privet leaves before and after the exchange of waste. Second, we evaluated whether foragers could perceive the avoidance cues directly at the dump by quantifying the visits of labeled foragers to the waste chamber. Finally, we asked whether foragers learn to specifically avoid untreated leaves of a plant after a confinement over 3 hours in the dump of subcolonies that were previously fed fungicide-treated leaves of that species. After the exchange of the waste chambers, workers from subcolonies that had access to waste from fungicide-treated privet leaves learned to avoid that plant. One-third of the labeled foragers visited the dump. Furthermore, naïve foragers learned to avoid a specific, previously unsuitable plant if exposed solely to cues of the dump during confinement. We suggest that cues at the dump enable foragers to predict the unsuitable effects of plants even if they had never been experienced in the fungus garden.

  2. Thin Places

    OpenAIRE

    Lockwood, Sandra Elizabeth

    2013-01-01

    This inquiry into the three great quests of the twentieth century–the South Pole, Mount Everest, and the Moon–examines our motivations to venture into these sublime, yet life-taking places. The Thin Place was once the destination of the religious pilgrim seeking transcendence in an extreme environment. In our age, the Thin Place quest has morphed into a challenge to evolve beyond the confines of our own physiology; through human ingenuity and invention, we reach places not meant to accommod...

  3. Molecular Mechanisms Regulating Sporulation in the Filamentous Fungus Ashbya gossypii

    DEFF Research Database (Denmark)

    Wasserstrom, Lisa

    , which is regulated by the pheromone response pathway. Most ascomycetes have been reported to produce meiotic spores, however, a sexual cycle has not yet been identified in the filamentous fungus Ashbya gossypii. The main focus of my doctoral thesis has therefore been to understand the mechanisms behind...... sporulation in this fungus.  The lifecycle of A. gossypii starting with a haploid spore that matures into spore-containing mycelia can be completed without the need for a mating partner. Spores in A. gossypii are thought to be derived sexually like all other Saccharomycetaceae species, but the sexual cycle...... suggesting that other proteins generate DSBs in this fungus. In summary, this work has led to better understanding of the components regulating sporulation in A. gossypii and their hierarchical organization....

  4. Effects of whey on the colonization and sporulation of arbuscular ...

    African Journals Online (AJOL)

    The aim of this study is to research the effect of 2 different doses of whey [50 ml kg-1(W50) and 100 ml kg-1(W100)], an important organic waste, on colonization and sporulation of arbuscular mycorhizal fungus (AMF) Glomus intraradices'(G.i.) inoculated to lentil plant and the effects of changing P ratio in the soil and plant as ...

  5. Light suppresses sporulation and epidemics of Peronospora belbahrii.

    Directory of Open Access Journals (Sweden)

    Yigal Cohen

    Full Text Available Peronospora belbahrii is a biotrophic oomycete attacking sweet basil. It propagates asexually by producing spores on dichotomously branched sporophores emerging from leaf stomata. Sporulation occurs when infected plants are incubated for at least 7.5h in the dark in moisture-saturated atmosphere at 10-27°C. Exposure to light suppresses spore formation but allows sporophores to emerge from stomata. Incandescent or CW fluorescent light of 3.5 or 6 µmoles.m(2.s(-1 respectively, caused 100% inhibition of spore formation on lower leaf surface even when only the upper leaf surface was exposed to light. The inhibitory effect of light failed to translocate from an illuminated part of a leaf to a shaded part of the same leaf. Inhibition of sporulation by light was temperature-dependent. Light was fully inhibitory at 15-27°C but not at 10°C, suggesting that enzyme(s activity and/or photoreceptor protein re-arrangement induced by light occur at ≥15°C. DCMU or paraquat could not abolish light inhibition, indicating that photosystem I and photosystem II are not involved. Narrow band led illumination showed that red light (λmax 625 nm was most inhibitory and blue light (λmax 440 nm was least inhibitory, suggesting that inhibition in P. belbahrii, unlike other oomycetes, operates via a red light photoreceptor. Nocturnal illumination of basil in the field (4-10 µmoles.m(2.s(-1 from 7pm to 7am suppressed sporulation of P. belbahrii and reduced epidemics of downy mildew, thus reducing the need for fungicide applications. This is the first report on red light inhibition of sporulation in oomycetes and on the practical application of light for disease control in the field.

  6. Temporal Expression of a Master Regulator Drives Synchronous Sporulation in Budding Yeast

    Directory of Open Access Journals (Sweden)

    Minghao Chia

    2016-11-01

    Full Text Available Yeast cells enter and undergo gametogenesis relatively asynchronously, making it technically challenging to perform stage-specific genomic and biochemical analyses. Cell-to-cell variation in the expression of the master regulator of entry into sporulation, IME1, has been implicated to be the underlying cause of asynchronous sporulation. Here, we find that timing of IME1 expression is of critical importance for inducing cells to undergo sporulation synchronously. When we force expression of IME1 from an inducible promoter in cells incubated in sporulation medium for 2 hr, the vast majority of cells exhibit synchrony during premeiotic DNA replication and meiotic divisions. Inducing IME1 expression too early or too late affects the synchrony of sporulation. Surprisingly, our approach for synchronous sporulation does not require growth in acetate-containing medium, but can be achieved in cells grown in rich medium until saturation. Our system requires solely IME1, because the expression of the N6-methyladenosine methyltransferase IME4, another key regulator of early sporulation, is controlled by IME1 itself. The approach described here can be combined easily with other stage-specific synchronization methods, and thereby applied to study specific stages of sporulation, or the complete sporulation program.

  7. Effect of health-promoting posters placed on the platforms of two train stations in Copenhagen, Denmark, on the choice between taking the stairs or the escalators

    DEFF Research Database (Denmark)

    Iversen, Mette Kathrine; Händel, M N; Nydal Jensen, Eva

    2007-01-01

    OBJECTIVE: The purpose of this study was to determine whether posters placed on the platforms of two train stations in Copenhagen, promoting use of the stairs, would encourage people to use the stairs rather than the adjacent escalator. An additional purpose was to see if the effect...

  8. Sporulation: how to survive on planet Earth (and beyond).

    Science.gov (United States)

    Huang, Mingwei; Hull, Christina M

    2017-10-01

    Sporulation is a strategy widely utilized by a wide variety of organisms to adapt to changes in their individual environmental niches and survive in time and/or space until they encounter conditions acceptable for vegetative growth. The spores produced by bacteria have been the subjects of extensive studies, and several systems such as Bacillus subtilis have provided ample opportunities to understand the molecular basis of spore biogenesis and germination. In contrast, the spores of other microbes, such as fungi, are relatively poorly understood. Studies of sporulation in model systems such as Saccharomyces cerevisiae and Aspergillus nidulans have established a basis for investigating eukaryotic spores, but very little is known at the molecular level about how spores function. This is especially true among the spores of human fungal pathogens such as the most common cause of fatal fungal disease, Cryptococcus neoformans. Recent proteomic studies are helping to determine the molecular mechanisms by which pathogenic fungal spores are formed, persist and germinate into actively growing agents of human disease.

  9. A new chemically defined medium for the growth and sporulation of Bacillus cereus strains in anaerobiosis.

    Science.gov (United States)

    Abbas, Amina Aicha; Planchon, Stella; Jobin, Michel; Schmitt, Philippe

    2014-10-01

    A new chemically defined liquid medium, MODS, was developed for the aerobic growth and anaerobic growth and sporulation of Bacillus cereus strains. The comparison of sporulation capacity of 18 strains of B. cereus has shown effective growth and spore production in anaerobiosis.. Copyright © 2014 Elsevier B.V. All rights reserved.

  10. Phosphatases modulate the bistable sporulation gene expression pattern in Bacillus subtilis

    NARCIS (Netherlands)

    Veening, JW; Hamoen, LW; Kuipers, OP

    Spore formation in the Gram- positive bacterium Bacillus subtilis is a last resort adaptive response to starvation. To initiate sporulation, the key regulator in this process, Spo0A, needs to be activated by the so-called phosphorelay. Within a sporulating culture of B. subtilis, some cells initiate

  11. Sporulation dynamics and spore heat resistance in wet and dry biofilms of Bacillus cereus

    NARCIS (Netherlands)

    Hayrapetyan, Hasmik; Abee, Tjakko; Nierop Groot, Masja

    2016-01-01

    Environmental conditions and growth history can affect the sporulation process as well as subsequent properties of formed spores. The sporulation dynamics was studied in wet and air-dried biofilms formed on stainless steel (SS) and polystyrene (PS) for Bacillus cereus ATCC 10987 and the

  12. NS [Nuclear Safety] update. Current safety and security activities and developments taking place in the Department of Nuclear Safety and Security, Issue no. 8, September 2008

    International Nuclear Information System (INIS)

    2008-09-01

    The current issue presents information about the following activities: 1) International Workshops on Denial of Shipments raise awareness of suppliers, recipients, regulators, carriers/consignors and international organizations of the problems relating to denials of radioactive shipments to determine effective measures to prevent or reduce the instances of shipment denials and delays. 2) Communication and knowledge Management in the Department of Nuclear Safety and Security (NS). 3) Nuclear Security at the Beijing Olympics - an excellent example of the IAEA's work in protecting large scale public events. 4) The Incident and Emergency Centre's Participation in the ConvEx 3 Exercise, 9-10 July 2008, which took place at the Laguna Verde nuclear power plant in Mexico. During the 43 hour long exercise, the Incident and Emergency Centre (IEC) was fully activated. Staff members participating in the exercise represented different departments within the IAEA and the diversity of their knowledge and experience ensured an effective response

  13. NS [Nuclear Safety] update. Current safety and security activities and developments taking place in the Department of Nuclear Safety and Security, Issue no. 6, March 2008

    International Nuclear Information System (INIS)

    2008-03-01

    The current issue presents information about the following activities: 1) International Conference on Illicit Nuclear Trafficking which took place in November 2007 in Edinburgh. The principal aim of the conference was to examine the threat and context of illicit nuclear trafficking of radioactive material, specifically, what is being done to combat such trafficking and where more needs to be done. The conference was also to consider how the obligations and commitments of the legally binding and non-binding international instruments could be and are being implemented by various States. 2) INSAG Message on Nuclear Safety Infrastructure in which the INSAG Chairman Richard Meserve addressed nuclear safety in the current context and various issues that warrant special attention. 3) approved for publication the Safety Requirements publication on Safety of Nuclear Fuel Cycle Facilities. 4) The Asian Nuclear Safety Network (ANSN)

  14. Radiation damage and its repair in non-sporulating bacteria

    International Nuclear Information System (INIS)

    Moseley, B.E.B.

    1984-01-01

    A review is given of radiation damage and its repair in non-sporulating bacteria. The identification and measurement of radiation damage in the DNA of the bacteria after exposure to ultraviolet radiation and ionizing radiation is described. Measuring the extent of DNA repair and ways of isolating repair mutants are also described. The DNA repair mechanisms for UV-induced damage are discussed including photoreactivation repair, excision repair, post-replication recombination repair and induced error-prone repair. The DNA repair mechanisms for ionizing radiation damage are also discussed including the repair of both single and double-strand breaks. Other aspects discussed include the effects of growth, irradiation medium and recovery medium on survival, DNA repair in humans, the commercial use of UV and ionizing radiations and the future of ionizing irradiation as a food treatment process. (U.K.)

  15. NS [Nuclear Safety] update. Current safety and security activities and developments taking place in the Department of Nuclear Safety and Security. Issue no. 2, January 2007

    International Nuclear Information System (INIS)

    2006-08-01

    This newsletter reports on the training of cardiologists in radiation protection, IAEA's safety review services and the operational safety assessment review team (OSART), the international conference on management of spent fuel and the recent INSAG (International Nuclear Safety Group) publications. The IAEA has begun a major international initiative to train interventional cardiologists in radiation protection. Starting with the first course in May 2004, so far 6 regional and 3 national training courses have been conducted with the participation of over 400 health professionals putting the IAEA in a leading role in this area. A programme of two days' training has been developed, covering possible and observed radiation effects among patients and staff, international standards, dose management techniques, examples of good and bad practice and examples indicating prevention of possible injuries as a result of good practice in radiation protection. The training material is freely available on CD and will be placed on the Radiological Protection of Patients website at http://rpop.iaea.org/

  16. Differential effects of sporulation temperature on the high pressure resistance of Clostridium botulinum type E spores and the interconnection with sporulation medium cation contents.

    Science.gov (United States)

    Lenz, Christian A; Vogel, Rudi F

    2015-04-01

    High pressure thermal (HPT) processing can be used to improve traditional preservation methods and increase food safety and durability, whereas quality related characteristics can be largely maintained. Clostridium (C.) botulinum type E is a non-proteolytic, psychrotrophic, toxin-producing spore former, commonly associated with aquatic environments in temperate regions of the northern hemisphere. Sporulation in nature is likely to occur under varying conditions including temperature and nutrient availability, which might affect resistance properties of resulting spores. In our study, we determined the effect of sporulation temperature (13-38 °C) on the resistance of three Clostridium botulinum type E strains to differently intense HPT treatments (200 MPa at 40 and 80 °C, and 800 MPa at 40 and 80 °C). Furthermore, the effect of cations on sporulation temperature-mediated alterations in HHP resistance was investigated. Results indicate that low and high sporulation temperatures can increase and decrease sporal HPT resistance, respectively, in a treatment-dependent (pressure level, treatment temperature) manner, whereas the trends observed are largely unaffected by pressure dwells (1 s-10 min). Furthermore, results show that the cation content of the sporulation medium (Ca(2+), Mg(2+), Mn(2+)) marginally influences and partially counteracts effects on the HPT resistance of spores grown at low and elevated temperatures, respectively. This suggests that sporulation temperature and medium cations provoke changes in some common spore resistance structures. Sporulation conditions can markedly affect spore resistance properties and, thus, should be considered for the experimental setup of worst case studies aiming to evaluate the effectiveness of food processes in terms of the inactivation of C. botulinum type E spores. Copyright © 2014 Elsevier Ltd. All rights reserved.

  17. Leaf processing behaviour in Atta leafcutter ants: 90% of leaf cutting takes place inside the nest, and ants select pieces that require less cutting.

    Science.gov (United States)

    Garrett, Ryan W; Carlson, Katherine A; Goggans, Matthew Scott; Nesson, Michael H; Shepard, Christopher A; Schofield, Robert M S

    2016-01-01

    Leafcutter ants cut trimmings from plants, carry them to their underground nests and cut them into smaller pieces before inoculating them with a fungus that serves as a primary food source for the colony. Cutting is energetically costly, so the amount of cutting is important in understanding foraging energetics. Estimates of the cutting density, metres of cutting per square metre of leaf, were made from samples of transported leaf cuttings and of fungal substrate from field colonies of Atta cephalotes and Atta colombica. To investigate cutting inside the nest, we made leaf-processing observations of our laboratory colony, A. cephalotes. We did not observe the commonly reported reduction of the leaf fragments into a pulp, which would greatly increase the energy cost of processing. Video clips of processing behaviours, including behaviours that have not previously been described, are linked. An estimated 2.9 (±0.3) km of cutting with mandibles was required to reduce a square metre of leaf to fungal substrate. Only about 12% (±1%) of this cutting took place outside of the nest. The cutting density and energy cost is lower for leaf material with higher ratios of perimeter to area, so we tested for, and found that the laboratory ants had a preference for leaves that were pre-cut into smaller pieces. Estimates suggest that the energy required to transport and cut up the leaf material is comparable to the metabolic energy available from the fungus grown on the leaves, and so conservation of energy is likely to be a particularly strong selective pressure for leafcutter ants.

  18. Lysogeny and Sporulation in Bacillus Isolates from the Gulf of Mexico▿ †

    Science.gov (United States)

    Mobberley, Jennifer; Authement, R. Nathan; Segall, Anca M.; Edwards, Robert A.; Slepecky, R. A.; Paul, J. H.

    2010-01-01

    Eleven Bacillus isolates from the surface and subsurface waters of the Gulf of Mexico were examined for their capacity to sporulate and harbor prophages. Occurrence of sporulation in each isolate was assessed through decoyinine induction, and putative lysogens were identified by prophage induction by mitomycin C treatment. No obvious correlation between ability to sporulate and prophage induction was found. Four strains that contained inducible virus-like particles (VLPs) were shown to sporulate. Four strains did not produce spores upon induction by decoyinine but contained inducible VLPs. Two of the strains did not produce virus-like particles or sporulate significantly upon induction. Isolate B14905 had a high level of virus-like particle production and a high occurrence of sporulation and was further examined by genomic sequencing in an attempt to shed light on the relationship between sporulation and lysogeny. In silico analysis of the B14905 genome revealed four prophage-like regions, one of which was independently sequenced from a mitomycin C-induced lysate. Based on PCR and transmission electron microscopy (TEM) analysis of an induced phage lysate, one is a noninducible phage remnant, one may be a defective phage-like bacteriocin, and two were inducible prophages. One of the inducible phages contained four putative transcriptional regulators, one of which was a SinR-like regulator that may be involved in the regulation of host sporulation. Isolates that both possess the capacity to sporulate and contain temperate phage may be well adapted for survival in the oligotrophic ocean. PMID:20008174

  19. Hypoxia and inactivity related physiological changes precede or take place in absence of significant rearrangements in bacterial community structure: The PlanHab randomized trial pilot study.

    Directory of Open Access Journals (Sweden)

    Robert Šket

    Full Text Available We explored the assembly of intestinal microbiota in healthy male participants during the randomized crossover design of run-in (5 day and experimental phases (21-day normoxic bed rest (NBR, hypoxic bed rest (HBR and hypoxic ambulation (HAmb in a strictly controlled laboratory environment, with balanced fluid and dietary intakes, controlled circadian rhythm, microbial ambiental burden and 24/7 medical surveillance. The fraction of inspired O2 (FiO2 and partial pressure of inspired O2 (PiO2 were 0.209 and 133.1 ± 0.3 mmHg for NBR and 0.141 ± 0.004 and 90.0 ± 0.4 mmHg for both hypoxic variants (HBR and HAmb; ~4000 m simulated altitude, respectively. A number of parameters linked to intestinal environment such as defecation frequency, intestinal electrical conductivity (IEC, sterol and polyphenol content and diversity, indole, aromaticity and spectral characteristics of dissolved organic matter (DOM were measured (64 variables. The structure and diversity of bacterial microbial community was assessed using 16S rRNA amplicon sequencing. Inactivity negatively affected frequency of defecation and in combination with hypoxia increased IEC (p < 0.05. In contrast, sterol and polyphenol diversity and content, various characteristics of DOM and aromatic compounds, the structure and diversity of bacterial microbial community were not significantly affected over time. A new in-house PlanHab database was established to integrate all measured variables on host physiology, diet, experiment, immune and metabolic markers (n = 231. The observed progressive decrease in defecation frequency and concomitant increase in IEC suggested that the transition from healthy physiological state towards the developed symptoms of low magnitude obesity-related syndromes was dose dependent on the extent of time spent in inactivity and preceded or took place in absence of significant rearrangements in bacterial microbial community. Species B. thetaiotamicron, B. fragilis, B

  20. Designated Places

    Data.gov (United States)

    California Department of Resources — Census 2000 Place Names provides a seamless statewide GIS layer of places, including census designated places (CDP), consolidated cities, and incorporated places,...

  1. Reconsidering the sporulation characteristics of hypervirulent Clostridium difficile BI/NAP1/027.

    Directory of Open Access Journals (Sweden)

    David A Burns

    Full Text Available Clostridium difficile is the leading cause of antibiotic-associated diarrhoea and a major burden to healthcare services worldwide. In recent years, C. difficile strains belonging to the BI/NAP1/027 type have become highly represented among clinical isolates. These so-called 'hypervirulent' strains are associated with outbreaks of increased disease severity, higher relapse rates and an expanded repertoire of antibiotic resistance. Spores, formed during sporulation, play a pivotal role in disease transmission and it has been suggested that BI/NAP1/027 strains are more prolific in terms of sporulation in vitro than 'non-epidemic' C. difficile types. Work in our laboratory has since provided credible evidence to the contrary suggesting that the strain-to-strain variation in C. difficile sporulation characteristics is not type-associated. However, the BI/NAP1/027 type is still widely stated to have an increased rate of sporulation. In this study, we analysed the sporulation rates of 53 C. difficile strains, the largest sample size used to-date in such a study, including 28 BI/NAP1/027 isolates. Our data confirm that significant variation exists in the rate at which different C. difficile strains form spores. However, we clearly show that the sporulation rate of the BI/NAP1/027 type was no higher than that of non-BI/NAP1/027 strains. In addition, we observed substantial variation in sporulation characteristics within the BI/NAP1/027 type. This work highlights the danger of assuming that all strains of one type behave similarly without studying adequate sample sizes. Furthermore, we stress the need for more rigorous experimental procedures in order to quantify C. difficile sporulation more accurately in the future.

  2. Identification of diacetonamine from soybean curd residue as a sporulation-inducing factor toward Bacillus spp.

    Science.gov (United States)

    Ikeda, Aki; Kim, Dongyeop; Hashidoko, Yasuyuki

    2017-12-01

    Under bioassay-guided investigation, a sporulation-inducing factor (SIF) toward Bacillus spp. was searched for in methanol (MeOH) extracts of soybean curd residues, and diacetonamine (1) was identified as the active compound. SIF was first isolated as a monoacetylated derivative (2, 4.1 mg from 655 g soybean curd residues), and its chemical structure was elucidated by field desorption mass spectrometry, electron ionization mass spectrometry, and nuclear magnetic resonance (NMR) analyses. After 48-h incubation, 40 µM diacetonamine hydrochloride (1b) exhibited sporulation-inducing activity with 35% sporulation frequency toward a Bacillus amyloliquefaciens wild-type strain (AHU 2170), whereas 40 µM diacetone acrylamide (3) showed 99% sporulation induction, which was much higher than that of 1b. Although Bacillus megaterium NBRC 15308 was sporulated by the treatment with 400 µM 1b with 36 and 70% sporulation frequency after 72- and 96-h incubation respectively, 3 at the same concentration showed only 2% sporulation after 72-h incubation. Hence, diacetonamine (1) was characterized as a genuine SIF from soybean curd residues, but it was uncertain whether 1 is a natural product or an artifact. Spores of B. amyloliquefaciens induced by 1b survived after treatment with heating at 95 °C for 10 min, also suggesting that 1 is genuine SIF in soybean curd residue. As sporulation induction is likely linked to activation of antibiotic production in some spore-forming Firmicutes bacteria, compound 1 would be a possible chemical tool to develop an effective fermentation technology in Bacillus species.

  3. Temperature and light intensity interaction on Cercospora coffeicola sporulation and conidia germination

    Directory of Open Access Journals (Sweden)

    Marília Goulart da Silva

    2016-04-01

    Full Text Available ABSTRACT Difficulty in obtaining abundant sporulation in culture of many species of Cercospora may be the limiting factor for studies of biology, systematics, and inoculation of the genus. Therefore, it is necessary to understand the nutritional and environmental requirements that influence mycelial growth, sporulation and germination. As it is difficult to obtain conidia of Cercospora coffeicola in vitro, different temperatures (17, 22, 27, and 32 °C and light intensities (80, 160, 240, and 320 μmol m-2 s-1 were evaluated to optimize pathogen sporulation and assess favorable conditions for spore germination, aiming for a strategy of disease control. The dark treatment (0 μmol m-2 s-1 was added for sporulation. A significant interaction was found between temperature and light intensity for both variables. The highest sporulation rate of C. coffeicola occurred at a light intensity of 240 μmol m-2 s-1 and air temperature of 22 °C, reaching 5.9x106 con mL-1. Germination was higher at temperature 17 °C and light intensity of 320 μmol m-2 s-1, reaching 52%. Interaction between light intensity and temperature proved to influence the processes of sporulation and germination of C. coffeicola.

  4. Myb transcription factors and light regulate sporulation in the oomycete Phytophthora infestans.

    Science.gov (United States)

    Xiang, Qijun; Judelson, Howard S

    2014-01-01

    Life cycle progression in eukaryotic microbes is often influenced by environment. In the oomycete Phytophthora infestans, which causes late blight on potato and tomato, sporangia have been reported to form mostly at night. By growing P. infestans under different light regimes at constant temperature and humidity, we show that light contributes to the natural pattern of sporulation by delaying sporulation until the following dark period. However, illumination does not permanently block sporulation or strongly affect the total number of sporangia that ultimately form. Based on measurements of sporulation-induced genes such as those encoding protein kinase Pks1 and Myb transcription factors Myb2R1 and Myb2R3, it appears that most spore-associated transcripts start to rise four to eight hours before sporangia appear. Their mRNA levels oscillate with the light/dark cycle and increase with the amount of sporangia. An exception to this pattern of expression is Myb2R4, which is induced several hours before the other genes and declines after cultures start to sporulate. Transformants over-expressing Myb2R4 produce twice the number of sporangia and ten-fold higher levels of Myb2R1 mRNA than wild-type, and chromatin immunoprecipitation showed that Myb2R4 binds the Myb2R1 promoter in vivo. Myb2R4 thus appears to be an early regulator of sporulation. We attempted to silence eight Myb genes by DNA-directed RNAi, but succeeded only with Myb2R3, which resulted in suppressed sporulation. Ectopic expression studies of seven Myb genes revealed that over-expression frequently impaired vegetative growth, and in the case of Myb3R6 interfered with sporangia dormancy. We observed that the degree of silencing induced by a hairpin construct was correlated with its copy number, and ectopic expression was often unstable due to epigenetic silencing and transgene excision.

  5. Myb transcription factors and light regulate sporulation in the oomycete Phytophthora infestans.

    Directory of Open Access Journals (Sweden)

    Qijun Xiang

    Full Text Available Life cycle progression in eukaryotic microbes is often influenced by environment. In the oomycete Phytophthora infestans, which causes late blight on potato and tomato, sporangia have been reported to form mostly at night. By growing P. infestans under different light regimes at constant temperature and humidity, we show that light contributes to the natural pattern of sporulation by delaying sporulation until the following dark period. However, illumination does not permanently block sporulation or strongly affect the total number of sporangia that ultimately form. Based on measurements of sporulation-induced genes such as those encoding protein kinase Pks1 and Myb transcription factors Myb2R1 and Myb2R3, it appears that most spore-associated transcripts start to rise four to eight hours before sporangia appear. Their mRNA levels oscillate with the light/dark cycle and increase with the amount of sporangia. An exception to this pattern of expression is Myb2R4, which is induced several hours before the other genes and declines after cultures start to sporulate. Transformants over-expressing Myb2R4 produce twice the number of sporangia and ten-fold higher levels of Myb2R1 mRNA than wild-type, and chromatin immunoprecipitation showed that Myb2R4 binds the Myb2R1 promoter in vivo. Myb2R4 thus appears to be an early regulator of sporulation. We attempted to silence eight Myb genes by DNA-directed RNAi, but succeeded only with Myb2R3, which resulted in suppressed sporulation. Ectopic expression studies of seven Myb genes revealed that over-expression frequently impaired vegetative growth, and in the case of Myb3R6 interfered with sporangia dormancy. We observed that the degree of silencing induced by a hairpin construct was correlated with its copy number, and ectopic expression was often unstable due to epigenetic silencing and transgene excision.

  6. Molecular Mechanisms Regulating Sporulation in the Filamentous Fungus Ashbya gossypii

    DEFF Research Database (Denmark)

    Wasserstrom, Lisa

    , which is regulated by the pheromone response pathway. Most ascomycetes have been reported to produce meiotic spores, however, a sexual cycle has not yet been identified in the filamentous fungus Ashbya gossypii. The main focus of my doctoral thesis has therefore been to understand the mechanisms behind...... remains unidentified. In this thesis I provide a comprehensive functional analysis of genes important for sporulation in A. gossypii. Previous results, together with findings presented in this work show that the role of the pheromone response pathway in A. gossypii has been rewired to regulate sporulation...... recombination that allows cross-over between homologous chromosomes. In S. cerevisiae, this requires double strand break (DSB) formation and subsequent repair via the components Spo11 and Dmc1. The work in this thesis show that the A. gossypii Spo11 and Dmc1 homologs are not required for sporulation, thus...

  7. Poly(3-hydroxybutyrate) fuels the tricarboxylic acid cycle and de novo lipid biosynthesis during Bacillus anthracis sporulation.

    Science.gov (United States)

    Sadykov, Marat R; Ahn, Jong-Sam; Widhelm, Todd J; Eckrich, Valerie M; Endres, Jennifer L; Driks, Adam; Rutkowski, Gregory E; Wingerd, Kevin L; Bayles, Kenneth W

    2017-06-01

    Numerous bacteria accumulate poly(3-hydroxybutyrate) (PHB) as an intracellular reservoir of carbon and energy in response to imbalanced nutritional conditions. In Bacillus spp., where PHB biosynthesis precedes the formation of the dormant cell type called the spore (sporulation), the direct link between PHB accumulation and efficiency of sporulation was observed in multiple studies. Although the idea of PHB as an intracellular carbon and energy source fueling sporulation was proposed several decades ago, the mechanisms underlying PHB contribution to sporulation have not been defined. Here, we demonstrate that PHB deficiency impairs Bacillus anthracis sporulation through diminishing the energy status of the cells and by reducing carbon flux into the tricarboxylic acid (TCA) cycle and de novo lipid biosynthesis. Consequently, this metabolic imbalance decreased biosynthesis of the critical components required for spore integrity and resistance, such as dipicolinic acid (DPA) and the spore's inner membrane. Supplementation of the PHB deficient mutant with exogenous fatty acids overcame these sporulation defects, highlighting the importance of the TCA cycle and lipid biosynthesis during sporulation. Combined, the results of this work reveal the molecular mechanisms of PHB contribution to B. anthracis sporulation and provide valuable insight into the metabolic requirements for this developmental process in Bacillus species. © 2017 John Wiley & Sons Ltd.

  8. Growth and sporulation of Bacillus cereus ATCC 14579 under defined conditions: temporal expression of genes for key sigma factors

    NARCIS (Netherlands)

    Vries, de Y.P.; Hornstra, L.M.; Vos, de W.M.; Abee, T.

    2004-01-01

    An airlift fermentor system allowing precise regulation of pH and aeration combined with a chemically defined medium was used to study growth and sporulation of Bacillus cereus ATCC 14579. Sporulation was complete and synchronous. Expression of sigA, sigB, sigF, and sigG was monitored with real-time

  9. Assessment of pheromone production and response in fission yeast by a halo test of induced sporulation

    DEFF Research Database (Denmark)

    Egel, R; Willer, M; Kjaerulff, S

    1994-01-01

    is assessed as an iodine-positive halo of sporulation surrounding the pheromone source, and the width of the halo is related to the amount of pheromone being produced. The assay is sufficiently sensitive to monitor the low amount of M-factor produced by an M mam1 strain, and its sensitivity towards P...

  10. Influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina

    Directory of Open Access Journals (Sweden)

    Diana Erica Gómez

    2013-06-01

    Full Text Available Fungi require special substrates for their isolation, vegetative growth and sporulation. In experiments conducted in the laboratory, the influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina conidia, the causal agent of soybean frogeye leaf spot, was assessed. The media potato sucrose agar, V-8 agar, tomato extract agar, soybean leaf extract agar, soybean seed extract agar, soybean meal agar, soybean flour agar and wheat flour agar were tested, added on the surface, with and without filter paper and under two light regimes, with 12 h light at 25°± 2°C and in the dark. A triple factorial 8x2x2 (substrates x light/dark x with/without filter paper design with four replicates was used. V-8 agar medium was employed and the pH was adjusted with HCl 0.1N or NaOH 0.1N before autoclaving to the values: 3, 4, 5, 6, 7 and 8, and the pH of V-8 agar medium is 6.7. The evaluation was done on the seventh day of incubation. Data underwent regression analysis. Sporulation was maximized on the agar media V-8, seed extract, oat flour, tomato extract, and potato sucrose in the presence of filter paper and 12h light. On V-8 medium, maximal sporulation was obtained with pH 6.7.

  11. Effect of plant sterols and tannins on Phytophthora ramorum growth and sporulation

    Science.gov (United States)

    Rachel A. Stong; Eli Kolodny; Rick G. Kelsey; M.P. Gonzalez-Hernandez; Jorge M. Vivanco; Daniel K. Manter

    2013-01-01

    Elicitin-mediated acquisition of plant sterols is required for growth and sporulation of Phytophthora spp. This study examined the interactions between elicitins, sterols, and tannins. Ground leaf tissue, sterols, and tannin-enriched extracts were obtained from three different plant species (California bay laurel, California black oak, and Oregon...

  12. SporeWeb : an interactive journey through the complete sporulation cycle of Bacillus subtilis

    NARCIS (Netherlands)

    Eijlander, Robyn T.; Jong, Anne de; Krawczyk, Antonina O.; Holsappel, Siger; Kuipers, Oscar P.

    2014-01-01

    Bacterial spores are a continuous problem for both food-based and health-related industries. Decades of scientific research dedicated towards understanding molecular and gene regulatory aspects of sporulation, spore germination and spore properties have resulted in a wealth of data and information.

  13. Effects of minerals on sporulation and heat resistance of Clostridium sporogenes.

    Science.gov (United States)

    Mah, Jae-Hyung; Kang, Dong-Hyun; Tang, Juming

    2008-12-10

    In this study, various mineral supplements, such as chloride salts (CaCl2, MgCl2, MnCl2, FeCl2 and KCl) supplying cations and calcium salts (CaCl2, CaCO3, CaSO4, Ca(OH)2 and CaHPO4) supplying anions, were tested if they could stimulate the sporulation of Clostridium sporogenes, a surrogate microorganism for C. botulinum. Of the cations tested, the addition of CaCl2 showed a slightly, but not significantly, greater increase in spore levels within 3 weeks of incubation, compared to that of the other cations. The optimum concentration of CaCl2 was 0.5%, which yielded nearly 10(4) CFU/ml of spores. Of the anions tested, CaCO3 promoted sporulation within one week, which was the most effective compound for promoting rapid sporulation among the minerals tested. CaSO4 produced a pattern of sporulation similar to that of CaCl2. While CaHPO4 resulted in the maximum production of spores after 4 weeks, Ca(OH)2 failed to induce sporulation. With an optimized concentration of 0.5% CaCO3, the spore yield was approximately 10(5) CFU/ml. The spores prepared in sporulation medium with CaCO3 (pH 5.0) had slightly, but not significantly, higher D values than those produced with CaCl2 (pH 5.0) at temperatures ranging from 113 to 121 degrees C. However, no significant differences were observed in Z values (both 10.76 degrees C). In a large scale spore production, D(121 degrees C) values of the spore crops prepared with CaCl2 and CaCO3 and resuspended in phosphate buffer (pH 7.0) were found to be both 0.92 min. In conclusion, our data suggest that CaCO3 is highly effective in reducing sporulation time as well as enhancing heat resistance.

  14. Connecting people to place

    NARCIS (Netherlands)

    Horlings, L.G.

    2016-01-01

    The article describes a process of preparing a research design on place-shaping, as outcome of a process of co-design between academic actors and non-academic actors in Brazil, South Africa and The Netherlands, taking place in the context of the project TRANSPLACE. The joint research design

  15. Women take the island: nation, profession, place Women take the island: nation, profession, place

    Directory of Open Access Journals (Sweden)

    Ruth Morse

    2008-04-01

    Full Text Available The Tempest has been one of Shakespeare’s most adapted plays. Its stage history is concomitantly a history of the British theatre, from regularized comedy to semi-opera to pantomime to opera. It has had other lives, too, from its position in romantic ideas of Shakespeare’s biography and his so-called farewell to the stage, to a supporting role as witness for the nineteenth-century Darwinians’ idea of the missing link, to a veritable efflorescence of walk-on parts, cameos, and star vehicles in twentieth-century psychoanalytic and social arguments about European expansion.2 The play has given us individual poems and paintings, not to speak of screen-plays for several film adaptations. The Tempest has been one of Shakespeare’s most adapted plays. Its stage history is concomitantly a history of the British theatre, from regularized comedy to semi-opera to pantomime to opera. It has had other lives, too, from its position in romantic ideas of Shakespeare’s biography and his so-called farewell to the stage, to a supporting role as witness for the nineteenth-century Darwinians’ idea of the missing link, to a veritable efflorescence of walk-on parts, cameos, and star vehicles in twentieth-century psychoanalytic and social arguments about European expansion.2 The play has given us individual poems and paintings, not to speak of screen-plays for several film adaptations.

  16. A Mutation in the Bacillus subtilis rsbU Gene That Limits RNA Synthesis during Sporulation.

    Science.gov (United States)

    Rothstein, David M; Lazinski, David; Osburne, Marcia S; Sonenshein, Abraham L

    2017-07-15

    Mutants of Bacillis subtilis that are temperature sensitive for RNA synthesis during sporulation were isolated after selection with a 32 P suicide agent. Whole-genome sequencing revealed that two of the mutants carried an identical lesion in the rsbU gene, which encodes a phosphatase that indirectly activates SigB, the stress-responsive RNA polymerase sigma factor. The mutation appeared to cause RsbU to be hyperactive, because the mutants were more resistant than the parent strain to ethanol stress. In support of this hypothesis, pseudorevertants that regained wild-type levels of sporulation at high temperature had secondary mutations that prevented expression of the mutant rsbU gene. The properties of these RsbU mutants support the idea that activation of SigB diminishes the bacterium's ability to sporulate. IMPORTANCE Most bacterial species encode multiple RNA polymerase promoter recognition subunits (sigma factors). Each sigma factor directs RNA polymerase to different sets of genes; each gene set typically encodes proteins important for responses to specific environmental conditions, such as changes in temperature, salt concentration, and nutrient availability. A selection for mutants of Bacillus subtilis that are temperature sensitive for RNA synthesis during sporulation unexpectedly yielded strains with a point mutation in rsbU , a gene that encodes a protein that normally activates sigma factor B (SigB) under conditions of salt stress. The mutation appears to cause RsbU, and therefore SigB, to be active inappropriately, thereby inhibiting, directly or indirectly, the ability of the cells to transcribe sporulation genes. Copyright © 2017 American Society for Microbiology.

  17. High-Throughput Genetic Screens Identify a Large and Diverse Collection of New Sporulation Genes in Bacillus subtilis

    Science.gov (United States)

    Brady, Jacqueline; Lim, Hoong Chuin; Bernhardt, Thomas G.; Rudner, David Z.

    2016-01-01

    The differentiation of the bacterium Bacillus subtilis into a dormant spore is among the most well-characterized developmental pathways in biology. Classical genetic screens performed over the past half century identified scores of factors involved in every step of this morphological process. More recently, transcriptional profiling uncovered additional sporulation-induced genes required for successful spore development. Here, we used transposon-sequencing (Tn-seq) to assess whether there were any sporulation genes left to be discovered. Our screen identified 133 out of the 148 genes with known sporulation defects. Surprisingly, we discovered 24 additional genes that had not been previously implicated in spore formation. To investigate their functions, we used fluorescence microscopy to survey early, middle, and late stages of differentiation of null mutants from the B. subtilis ordered knockout collection. This analysis identified mutants that are delayed in the initiation of sporulation, defective in membrane remodeling, and impaired in spore maturation. Several mutants had novel sporulation phenotypes. We performed in-depth characterization of two new factors that participate in cell–cell signaling pathways during sporulation. One (SpoIIT) functions in the activation of σE in the mother cell; the other (SpoIIIL) is required for σG activity in the forespore. Our analysis also revealed that as many as 36 sporulation-induced genes with no previously reported mutant phenotypes are required for timely spore maturation. Finally, we discovered a large set of transposon insertions that trigger premature initiation of sporulation. Our results highlight the power of Tn-seq for the discovery of new genes and novel pathways in sporulation and, combined with the recently completed null mutant collection, open the door for similar screens in other, less well-characterized processes. PMID:26735940

  18. The dev Operon Regulates the Timing of Sporulation during Myxococcus xanthus Development.

    Science.gov (United States)

    Rajagopalan, Ramya; Kroos, Lee

    2017-05-15

    Myxococcus xanthus undergoes multicellular development when starved. Thousands of rod-shaped cells coordinate their movements and aggregate into mounds in which cells differentiate into spores. Mutations in the dev operon impair development. The dev operon encompasses a clustered regularly interspaced short palindromic repeat-associated (CRISPR-Cas) system. Null mutations in devI , a small gene at the beginning of the dev operon, suppress the developmental defects caused by null mutations in the downstream devR and devS genes but failed to suppress defects caused by a small in-frame deletion in devT We provide evidence that the original mutant has a second-site mutation. We show that devT null mutants exhibit developmental defects indistinguishable from devR and devS null mutants, and a null mutation in devI suppresses the defects of a devT null mutation. The similarity of DevTRS proteins to components of the CRISPR-associated complex for antiviral defense (Cascade), together with our molecular characterization of dev mutants, support a model in which DevTRS form a Cascade-like subcomplex that negatively autoregulates dev transcript accumulation and prevents DevI overproduction that would strongly inhibit sporulation. Our results also suggest that DevI transiently inhibits sporulation when regulated normally. The mechanism of transient inhibition may involve MrpC, a key transcription factor, whose translation appears to be weakly inhibited by DevI. Finally, our characterization of a devI devS mutant indicates that very little exo transcript is required for sporulation, which is surprising since Exo proteins help form the polysaccharide spore coat. IMPORTANCE CRISPR-Cas systems typically function as adaptive immune systems in bacteria. The dev CRISPR-Cas system of M. xanthus has been proposed to prevent bacteriophage infection during development, but how dev controls sporulation has been elusive. Recent evidence supported a model in which DevR and DevS prevent

  19. Time Series Analysis of theBacillus subtilisSporulation Network Reveals Low Dimensional Chaotic Dynamics.

    Science.gov (United States)

    Lecca, Paola; Mura, Ivan; Re, Angela; Barker, Gary C; Ihekwaba, Adaoha E C

    2016-01-01

    Chaotic behavior refers to a behavior which, albeit irregular, is generated by an underlying deterministic process. Therefore, a chaotic behavior is potentially controllable. This possibility becomes practically amenable especially when chaos is shown to be low-dimensional, i.e., to be attributable to a small fraction of the total systems components. In this case, indeed, including the major drivers of chaos in a system into the modeling approach allows us to improve predictability of the systems dynamics. Here, we analyzed the numerical simulations of an accurate ordinary differential equation model of the gene network regulating sporulation initiation in Bacillus subtilis to explore whether the non-linearity underlying time series data is due to low-dimensional chaos. Low-dimensional chaos is expectedly common in systems with few degrees of freedom, but rare in systems with many degrees of freedom such as the B. subtilis sporulation network. The estimation of a number of indices, which reflect the chaotic nature of a system, indicates that the dynamics of this network is affected by deterministic chaos. The neat separation between the indices obtained from the time series simulated from the model and those obtained from time series generated by Gaussian white and colored noise confirmed that the B. subtilis sporulation network dynamics is affected by low dimensional chaos rather than by noise. Furthermore, our analysis identifies the principal driver of the networks chaotic dynamics to be sporulation initiation phosphotransferase B (Spo0B). We then analyzed the parameters and the phase space of the system to characterize the instability points of the network dynamics, and, in turn, to identify the ranges of values of Spo0B and of the other drivers of the chaotic dynamics, for which the whole system is highly sensitive to minimal perturbation. In summary, we described an unappreciated source of complexity in the B. subtilis sporulation network by gathering

  20. Places Connected:

    DEFF Research Database (Denmark)

    Hansen, Annette Skovsted

    experiences of place, however, when it is often the same people who experience many different places? Along with many other so-called donors in the 1950s, Denmark and Japan chose to invest in the education of own and other nationals involved in development and thereby financed personal connections between...

  1. Better Place

    DEFF Research Database (Denmark)

    Rask, Morten; Bakke, Nikolas; Lindhøj, Jan

    Better Place is trying to reshape the automotive industry by shifting transportation from a dependency on oil to a reliance on environmentally friendly renewable energy. Better Place is developing an extensive infrastructure system that will utilise overcapacity in the production of wind power...... among others and that will drive the global transportation industry to becoming driven by electric vehicles (EVs). Better Place does this by selling its customers 'mileage' and a car without a battery. The case highlights the internationalisation process of Better Place from an international business...... perspective in order to encourage a discussion and debate about how Better Place can make their grand vision a reality in the future by overcoming the obstacles that historically have been challenging the rise of the EV industry. The case includes a historical background of the EV industry by using Denmark...

  2. Environmental factors affecting sporulation of Fuligo septica (Myxomycetes on sugar cane bagasse

    Directory of Open Access Journals (Sweden)

    Chiappeta Alda de Andrade

    2003-01-01

    Full Text Available The influence of environmental factors on sporulation of Fuligo septica (L. Wigg. and the abundance of this species on sugar cane bagasse (Saccharum officinarum L., stored outdoors was studied.In Northeastern Brazil, between January/1997 and January/1998, a total of 29 specimens were collected through monthly collections of aethalia. The relationships between the abundance of aethalia and rainfall, temperature, relative humidity of the air and insolation were studied. Results indicated that on the substrate analyzed, F. septica was an abundant species. Sporulation occurred in all seasons of the year, with a well-defined peak at the end of winter and beginning of spring (August/September,which was strongly influenced by rainfall.

  3. Growth and sporulation of Metarhizium flavoviride var. Flavoviride on culture media and lighting regimes

    Directory of Open Access Journals (Sweden)

    Onofre Sideney Becker

    2001-01-01

    Full Text Available Entomopathogenic fungi from the genus Metarhizium are largely used for the biological control of agricultural pests by conidia spreading on the field. Although conidia production is well studied in M. anisopliae, only few research studies were done in M. flavoviride. The present work was carried out alming to evaluate the Mycelial growth and sporulation of the entomopathogenic fungus Metarhizium flavoviride var. flavoviride growing at 27 ± 2°C on Potato-dextrose-agar (PDA, Czapek-agar (CZP and a complete agar medium (CM under three lighting regimes, (continuous illumination, light/dark cycle and an black light/dark cycle were investigated. A completely randomized 3 × 3 (culture media × lighting regime factorial design with four replicates was used. The best mycelial growth and sporulation occurred on the PDA and CM media under continuous illumination (P <= 0,05.

  4. Sporulation of Metarhizium anisopliae var. Acridum and Beauveria bassiana on Rhammatocerus schistocercoides under humid and dry conditions

    Directory of Open Access Journals (Sweden)

    Magalhães Bonifácio Peixoto

    2000-01-01

    Full Text Available The sporulation of the fungi Metarhizium anisopliae var. acridum and Beauveria bassiana in cadavers of the grasshopper Rhammatocerus schistocercoides was studied in dry and humid environments. Both fungi were equally virulent against R. schistocercoides. However, internally, M. anisopliae produced more conidia than B. bassiana at 53% and 75% relative humidity. Externally, there was no sporulation at 53% and 75% RH, and M. anisopliae produced more conidia than B. bassiana at 100% RH.

  5. Les bactéries sporulées dans les conserves de légumes (petits pois ...

    African Journals Online (AJOL)

    Unicornis

    sporulées pouvant gêner l'innovation des procédés de fabrication industrielle [2]. La présence de B. ... cours des traitements technologiques de conservation. Ces bactéries sporulées ont probablement été sélectionnées par les process technologiques ou pourraient s'être adaptées à ces derniers; trouvant ainsi un terrain ...

  6. Place Branding

    DEFF Research Database (Denmark)

    Medway, Dominic; Swanson, Kathryn; Neirotti, Lisa Delpy

    2015-01-01

    Purpose: – The purpose of this paper is to report on a special session entitled “Place branding: Are we wasting our time?”, held at the American Marketing Association’s Summer Marketing Educators’ conference in 2014. Design/methodology/approach: – The report details the outcome of an Oxford-style...... of place branding towards an activity centred on “curation”. Originality/value: – The use of a competitive debating format as a means for exploring academic ideas and concepts in the place management field.......Purpose: – The purpose of this paper is to report on a special session entitled “Place branding: Are we wasting our time?”, held at the American Marketing Association’s Summer Marketing Educators’ conference in 2014. Design/methodology/approach: – The report details the outcome of an Oxford......: – The outcome of the debate points towards a need for place brands to develop as more inclusive and organic entities, in which case it may be best for place practitioners to avoid creating and imposing a place brand and instead help shape it from the views of stakeholder constituencies. This shifts the notion...

  7. Switch-like reprogramming of gene expression after fusion of multinucleate plasmodial cells of two Physarum polycephalum sporulation mutants

    Energy Technology Data Exchange (ETDEWEB)

    Walter, Pauline; Hoffmann, Xenia-Katharina; Ebeling, Britta; Haas, Markus; Marwan, Wolfgang, E-mail: wolfgang.marwan@ovgu.de

    2013-05-24

    Highlights: •We investigate reprogramming of gene expression in multinucleate single cells. •Cells of two differentiation control mutants are fused. •Fused cells proceed to alternative gene expression patterns. •The population of nuclei damps stochastic fluctuations in gene expression. •Dynamic processes of cellular reprogramming can be observed by repeated sampling of a cell. -- Abstract: Nonlinear dynamic processes involving the differential regulation of transcription factors are considered to impact the reprogramming of stem cells, germ cells, and somatic cells. Here, we fused two multinucleate plasmodial cells of Physarum polycephalum mutants defective in different sporulation control genes while being in different physiological states. The resulting heterokaryons established one of two significantly different expression patterns of marker genes while the plasmodial halves that were fused to each other synchronized spontaneously. Spontaneous synchronization suggests that switch-like control mechanisms spread over and finally control the entire plasmodium as a result of cytoplasmic mixing. Regulatory molecules due to the large volume of the vigorously streaming cytoplasm will define concentrations in acting on the population of nuclei and in the global setting of switches. Mixing of a large cytoplasmic volume is expected to damp stochasticity when individual nuclei deliver certain RNAs at low copy number into the cytoplasm. We conclude that spontaneous synchronization, the damping of molecular noise in gene expression by the large cytoplasmic volume, and the option to take multiple macroscopic samples from the same plasmodium provide unique options for studying the dynamics of cellular reprogramming at the single cell level.

  8. The RecA-Dependent SOS Response Is Active and Required for Processing of DNA Damage during Bacillus subtilis Sporulation.

    Directory of Open Access Journals (Sweden)

    Fernando H Ramírez-Guadiana

    Full Text Available The expression of and role played by RecA in protecting sporulating cells of Bacillus subtilis from DNA damage has been determined. Results showed that the DNA-alkylating agent Mitomycin-C (M-C activated expression of a PrecA-gfpmut3a fusion in both sporulating cells' mother cell and forespore compartments. The expression levels of a recA-lacZ fusion were significantly lower in sporulating than in growing cells. However, M-C induced levels of ß-galactosidase from a recA-lacZ fusion ~6- and 3-fold in the mother cell and forespore compartments of B. subtilis sporangia, respectively. Disruption of recA slowed sporulation and sensitized sporulating cells to M-C and UV-C radiation, and the M-C and UV-C sensitivity of sporangia lacking the transcriptional repair-coupling factor Mfd was significantly increased by loss of RecA. We postulate that when DNA damage is encountered during sporulation, RecA activates the SOS response thus providing sporangia with the repair machinery to process DNA lesions that may compromise the spatio-temporal expression of genes that are essential for efficient spore formation.

  9. In-planta Sporulation Capacity Enhances Infectivity and Rhizospheric Competitiveness of Frankia Strains.

    Science.gov (United States)

    Cotin-Galvan, Laetitia; Pozzi, Adrien C; Schwob, Guillaume; Fournier, Pascale; Fernandez, Maria P; Herrera-Belaroussi, Aude

    2016-01-01

    Frankia Sp+ strains maintain their ability to sporulate in symbiosis with actinorhizal plants, producing abundant sporangia inside host plant cells, in contrast to Sp- strains, which are unable to perform in-planta sporulation. We herein examined the role of in-planta sporulation in Frankia infectivity and competitiveness for root infection. Fifteen strains belonging to different Sp+ and Sp- phylogenetic lineages were inoculated on seedlings of Alnus glutinosa (Ag) and A. incana (Ai). Strain competitiveness was investigated by performing Sp-/Sp+ co-inoculations. Plant inoculations were standardized using crushed nodules obtained under laboratory-controlled conditions (same plant species, age, and environmental factors). Specific oligonucleotide primers were developed to identify Frankia Sp+ and/or Sp- strains in the resulting nodules. Single inoculation experiments showed that (i) infectivity by Sp+ strains was significantly greater than that by Sp- strains, (ii) genetically divergent Sp+ strains exhibited different infective abilities, and (iii) Sp+ and Sp- strains showed different host preferences according to the origin (host species) of the inocula. Co-inoculations of Sp+ and Sp- strains revealed the greater competitiveness of Sp+ strains (98.3 to 100% of Sp+ nodules, with up to 15.6% nodules containing both Sp+ and Sp- strains). The results of the present study highlight differences in Sp+/Sp- strain ecological behaviors and provide new insights to strengthen the obligate symbiont hypothesis for Sp+ strains.

  10. Two distinct pathways lead Bacillus thuringiensis to commit to sporulation in biofilm.

    Science.gov (United States)

    Verplaetse, Emilie; Slamti, Leyla; Gohar, Michel; Lereclus, Didier

    2017-05-01

    The spore-forming bacterium Bacillus thuringiensis is an efficient biofilm producer, responsible for persistent contamination of industrial food processing systems. B. thuringiensis biofilms are highly heterogeneous bacterial structures in which three distinct cell types controlled by quorum sensing regulators were identified: PlcR-controlled virulent cells, NprR-dependent necrotrophic cells and cells committed to sporulation, a differentiation process controlled by Rap phosphatases and Spo0A-P. Interestingly, a cell lineage study revealed that, in LB medium or in insect larvae, only necrotrophic cells became spores. Here we analyzed cellular differentiation undertaken by cells growing in biofilm in a medium optimized for sporulation. No virulent cells were identified; surprisingly, two distinct routes could lead to differentiation as a spore in this growth condition: the NprR-dependent route, followed by the majority of cells, and the newly identified NprR-independent route, which is followed by 20% of sporulating cells. Copyright © 2016. Published by Elsevier Masson SAS.

  11. Place (National)

    Data.gov (United States)

    Department of Transportation — This map layer includes cities and towns in the United States, Puerto Rico, and the U.S. Virgin Islands (NTAD). A city or town is a place with a recorded population,...

  12. Healthy Places

    Centers for Disease Control (CDC) Podcasts

    2007-04-10

    Every person has a stake in environmental public health. As the environment deteriorates, so does the physical and mental health of the people within it. Healthy places are those designed and built to improve the quality of life for all people who live, work, worship, learn, and play within their borders -- where every person is free to make choices amid a variety of healthy, available, accessible, and affordable options. The CDC recognizes significant health issues and places that are vital in developing the Healthy Places program and provides examples in this report.  Created: 4/10/2007 by CDC National Center for Environmental Health.   Date Released: 4/13/2007.

  13. Secret Places.

    Science.gov (United States)

    Ridolfi, Kerry

    1997-01-01

    Argues that children are as deep as the ocean, with secret places inside of them waiting to be opened. Notes that it is powerful for students to learn they can make sense of the world through words, and describes inviting them into poetry as they read poetry, create poetry packets, and write and revise poems. (SR)

  14. Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: ACCESS 2000 - niveau 1 : 13 & 14.11.03 (2 jours) C++ for Particle Physicists : 17 – 21.11.03 (6 X 3-hour lectures) Programmation automate Schneider TSX Premium – niveau 2 : 18 – 21.11.03 (4 jours) JAVA 2 Enterprise Edition – Part 1 : WEB Applications : 20 & ...

  15. Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Places available The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses : Introduction à Outlook : 19.8.2004 (1 journée) Outlook (short course I) : E-mail : 31.8.2004 (2 hours, morning) Outlook (short course II) : Calendar, Tasks and Notes : 31.8.2004 (2 hours, afternoon) Instructor-led WBTechT Study or Follow-up for Microsoft Applications : 7.9.2004 (morning) Outlook (short course III) : Meetings and Delegation : 7.9.2004 (2 hours, afternoon) Introduction ...

  16. Places disponibles/Places available

    CERN Multimedia

    2004-01-01

    Etant donné le délai d'impression du Bulletin, ces places peuvent ne plus être disponibles au moment de sa parution. Veuillez consulter notre site Web pour avoir la dernière mise à jour. The number of places available may vary. Please check our Web site to find out the current availability. Des places sont disponibles dans les cours suivants : / Places are available in the following courses : Introduction à Outlook : 19.8.2004 (1 journée) Outlook (short course I) : E-mail : 31.8.2004 (2 hours, morning) Outlook (short course II) : Calendar, Tasks and Notes : 31.8.2004 (2 hours, afternoon) Instructor-led WBTechT Study or Follow-up for Microsoft Applications : 7.9.2004 (morning) Outlook (short course III) : Meetings and Delegation : 7.9.2004 (2 hours, afternoon) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 7 & 8.9.2004 (2 jours) Joint PVSS JCOP Framework : 13 - 17.9.2004 (5 days) AutoCAD 2002 - niveau 1 : 13, 14, 23, 24.9.2004 (4 jours) Programmation S...

  17. Neurotoxin synthesis is positively regulated by the sporulation transcription factor Spo0A in Clostridium botulinum type E.

    Science.gov (United States)

    Mascher, Gerald; Mertaoja, Anna; Korkeala, Hannu; Lindström, Miia

    2017-10-01

    Clostridium botulinum produces the most potent natural toxin, the botulinum neurotoxin (BoNT), probably to create anaerobiosis and nutrients by killing the host, and forms endospores that facilitate survival in harsh conditions and transmission. Peak BoNT production coincides with initiation of sporulation in C. botulinum cultures, which suggests common regulation. Here, we show that Spo0A, the master regulator of sporulation, positively regulates BoNT production. Insertional inactivation of spo0A in C. botulinum type E strain Beluga resulted in significantly reduced BoNT production and in abolished or highly reduced sporulation in relation to wild-type controls. Complementation with spo0A restored BoNT production and sporulation. Recombinant DNA-binding domain of Spo0A directly bound to a putative Spo0A-binding box (CTTCGAA) within the BoNT/E operon promoter, demonstrating direct regulation. Spo0A is the first neurotoxin regulator reported in C. botulinum type E. Unlike other C. botulinum strains that are terrestrial and employ the alternative sigma factor BotR in directing BoNT expression, C. botulinum type E strains are adapted to aquatic ecosystems, possess distinct epidemiology and lack BotR. Our results provide fundamental new knowledge on the genetic control of BoNT production and demonstrate common regulation of BoNT production and sporulation, providing a key intervention point for control. © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.

  18. Δ(1-pyrroline-5-carboxylate/glutamate biogenesis is required for fungal virulence and sporulation.

    Directory of Open Access Journals (Sweden)

    Ziting Yao

    Full Text Available Proline dehydrogenase (Prodh and Δ(1-pyrroline-5-carboxylate dehydrogenase (P5Cdh are two key enzymes in the cellular biogenesis of glutamate. Recombinant Prodh and P5Cdh proteins of the chestnut blight fungus Cryphonectria parasitica were investigated and showed activity in in vitro assays. Additionally, the C. parasitica Prodh and P5Cdh genes were able to complement the Saccharomyces cerevisiae put1 and put2 null mutants, respectively, to allow these proline auxotrophic yeast mutants to grow on media with proline as the sole source of nitrogen. Deletion of the Prodh gene in C. parasitica resulted in hypovirulence and a lower level of sporulation, whereas deletion of P5Cdh resulted in hypovirulence though no effect on sporulation; both Δprodh and Δp5cdh mutants were unable to grow on minimal medium with proline as the sole nitrogen source. In a wild-type strain, the intracellular level of proline and the activity of Prodh and P5Cdh increased after supplementation of exogenous proline, though the intracellular Δ(1-pyrroline-5-carboxylate (P5C content remained unchanged. Prodh and P5Cdh were both transcriptionally down-regulated in cells infected with hypovirus. The disruption of other genes with products involved in the conversion of arginine to ornithine, ornithine and glutamate to P5C, and P5C to proline in the cytosol did not appear to affect virulence; however, asexual sporulation was reduced in the Δpro1 and Δpro2 mutants. Taken together, our results showed that Prodh, P5Cdh and related mitochondrial functions are essential for virulence and that proline/glutamate pathway components may represent down-stream targets of hypovirus regulation in C. parasitica.

  19. Metabolic alterations in broiler chickens experimentally infected with sporulated oocysts of Eimeria maxima

    OpenAIRE

    Freitas,Fagner Luiz da Costa

    2014-01-01

    Metabolic and morphometric alterations of the duodenal villi caused by parasitism of chickens by Eimeria maxima were evaluated, using 100 male Cobb birds, randomly distributed into two groups (control and infected). The infected group was inoculated with 0.5 ml of a solution containing 5×103 sporulated oocysts of Eimeria maxima. Ten birds per sample were sacrificed on the 6th, 11th, 22nd and 41st days post-infection (dpi). In order to evaluate the alterations, samples of duodenum, jejunum and...

  20. Comparison of the pathogenicity, growth, sporulation and morphology of Pezicula alba Guthrie (Gloeosporium album Osterw.

    Directory of Open Access Journals (Sweden)

    H. Borecka

    2013-12-01

    Full Text Available Sixteen isolates of Pezicula alba Guthr. were examined. The intensity of growth on various media, pathogenicity to apple fruits and twigs, colour of cultures and size of conidia were measured. Some isolates are pathogenic to fruits, some others to twigs; one isolate (only no. 19 is pathogenic to both twigs and fruits; many isolates are not - pathogenic at all. Culture growth, sporulation and size of conidia are not correlated with the pathogenicity of the isolate. The mean size of conidia is 21.29µm x 3.48µm.

  1. Post-mortem sporulation of Ceratomyxa shasta (Myxozoa) after death in adult Chinook salmon

    Science.gov (United States)

    Kent, Michael L.; Soderlund, K.; Thomann, E.; Schreck, Carl B.; Sharpton, T.J.

    2014-01-01

    Ceratomyxa shasta (Myxozoa) is a common gastrointestinal pathogen of salmonid fishes in the Pacific Northwest of the United States. We have been investigating this parasite in adult Chinook salmon (Oncorhynchus tshawytscha) in the Willamette River, Oregon. In prior work, we observed differences in the pattern of development of C. shasta in adult salmon compared to juvenile salmon. Adult salmon consistently had large numbers of prespore stages in many of the fish that survived to spawn in the fall. However, myxospores were rarely observed, even though they were exposed and presumably infected for months before spawning. We evaluated the ability of C. shasta to sporulate following fish death because it is reported that myxosores are common in carcasses of Chinook salmon. We collected the intestine from 30 adult salmon immediately after artificial spawning and death (T0). A total of 23 fish were infected with C. shasta based on histology, but only a few myxospores were observed in 1 fish by histology. Intestines of these fish were examined at T0 and T7 (latter held at 17 C for 7 days) using quantified wet mount preparations. An increase in myxospore concentrations was seen in 39% of these fish, ranging between a 1.5- to a 14.5-fold increase. The most heavily infected fish exhibited a 4.6-fold increase from 27,841 to 129,352 myxospores/cm. This indicates, supported by various statistical analyses, that under certain conditions presporogonic forms are viable and continue to sporulate after death in adult salmon. Considering the life cycle of C. shasta and anadromous salmon, the parasite may have evolved 2, non-mutually exclusive developmental strategies. In young fish (parr and smolts), the parasite sporulates shortly after infection and is released into freshwater from either live or dead fish before their migration to seawater, where the alternate host is absent. The second strategy occurs in adult salmon, particularly spring Chinook salmon, which become infected upon

  2. Envisioning place

    DEFF Research Database (Denmark)

    Schmidt, Garbi; Glick Schiller, Nina

    2016-01-01

    together, the articles contribute to an emerging relational social science by approaching urban sociabilities through four interrelated parameters: (1) a concept of place-making situated within trajectories of differential and multiscalar power; (2) a discursive analysis of narratives and silences......, including those about diversity and cultural difference, formulated by actors within different scales of power; (3) an analysis of how different temporalities make visible or invisible the presence, agency and interconnection of various actors engaged in city-making; and (4) a re-engagement with the notion...

  3. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74460

    2001-01-01

    Places are available in the following courses: Nouveautés de FileMaker : 20 - 23.03.01 (4 matins) Contract Follow-up : 9.4.01 (3 heures) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

  4. Designed Places

    DEFF Research Database (Denmark)

    Stender, Marie

    like the forces of nature. Based on a fieldwork in three new housing developments in Copenhagen, this paper aims to bring the financial crisis ‘down to earth’ by examining the material shapes it has taken in the spaces of everyday life. The ethnographic cases in question are places that are somewhat...... over-designed: Not only the places’ architecture and built environment but also their identity, image and social life has been carefully planned for. At the same time their design and architectural layout bear witness to a seemingly longing for that which is unplanned and beyond design, thus staging...

  5. Places available **

    CERN Multimedia

    2003-01-01

    Des places sont disponibles dans les cours suivants : Places are available in the following courses : WorldFIP 2003 pour utilisateurs : 11-14.2.03 (4 jours) DISP-2003 ? Spring I Term : Introduction to Digital Signal Processing : 20, 27.2, 6, 13, 20, 27.3, 3.4.03 (7 X 2-hour lectures) AXEL-2003 - Introduction to Accelerators : 24-28.2.03 (10 X 1-hour lectures) AutoCAD 2002 - niveau 1 : 24, 25.2 & 3, 4.3.03 (4 jours) Introduction à Windows 2000 au CERN : 25.2.03 (1/2 journée) LabView base 2/LabView Basics 2 : 10 & 11.3.03 (2 jours/2 days) langue à définir/Language to be decided C++ for Particle Physicists : 10 ? 14.3.03 (6 X 3-hour lectures) Introduction to PVSS : 10.3.03 (half day, afternoon) Basic PVSS : 11 - 13.3.03 (3 days) LabView avancé /LabView Advanced : 12 - 14.3.03 (3 jours/3days) Langue à définir/language to be decided AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 17, 18, 24 & 25.3.03 (6 jours) PVSS - JCOP Framework Tutorial : 14.3.03 (1 day) MAGNE-03 - Magnetism for Technical Ele...

  6. Places available **

    CERN Multimedia

    2003-01-01

    Des places sont disponibles dans les cours suivants : Places are available in the following courses : Introduction à Windows 2000 au CERN : 25.2.03 (1/2 journée) LabView base 2/LabView Basics 2 : 10 & 11.3.03 (2 jours/2 days) langue à définir/Language to be decided C++ for Particle Physicists : 10 - 14.3.03 (6 X 3-hour lectures) Introduction to PVSS : 10.3.03 (half day, afternoon) Basic PVSS : 11 - 13.3.03 (3 days) LabView avancé /LabView Advanced : 12 - 14.3.03 (3 jours/3days) Langue à définir/Language to be decided AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 17, 18, 24 & 25.3.03 (6 jours) PVSS - JCOP Framework Tutorial : 14.3.03 (1 day) CLEAN-2002 : Working in a cleanroom : 2.4.03 (half-day, afternoon, free course, registration required) LabView base 1/LabView Basics 1 : 9 - 11.4.03 (3 jours/3 days) Langue à définir/Language to be decided DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (...

  7. Places available **

    CERN Multimedia

    2003-01-01

    Des places sont disponibles dans les cours suivants : Places are available in the following courses : DISP-2003 - Spring I Term : Introduction to Digital Signal Processing : 20, 27.2, 6, 13, 20, 27.3, 3.4.03 (7 X 2-hour lectures) AXEL-2003 - Introduction to Accelerators : 24 - 28.2.03 (10 X 1-hour lectures) AutoCAD 2002 - niveau 1 : 24, 25.2 & 3, 4.3.03 (4 jours) Introduction à Windows 2000 au CERN : 25.2.03 (1/2 journée) LabView base 2/LabView Basics 2 : 10 & 11.3.03 (2 jours/2 days) langue à définir/Language to be decided C++ for Particle Physicists : 10 - 14.3.03 (6 X 3-hour lectures) Introduction to PVSS : 10.3.03 (half day, afternoon) Basic PVSS : 11 - 13.3.03 (3 days) LabView avancé /LabView Advanced : 12 - 14.3.03 (3 jours/3days) Langue à définir/language to be decided AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 17, 18, 24 & 25.3.03 (6 jours) PVSS - JCOP Framework Tutorial : 14.3.03 (1 day) CLEAN-2002 : Working in a cleanroom : 2.4.03 (half-day, afternoon, free course, regis...

  8. Places available **

    CERN Multimedia

    2003-01-01

    Des places sont disponibles dans les cours suivants : Places are available in the following courses : C++ for Particle Physicists : 10 - 14.3.03 (6 X 3-hour lectures) Introduction to PVSS : 10.3.03 (half day, afternoon) Basic PVSS : 11 - 13.3.03 (3 days) PVSS - JCOP Framework Tutorial : 14.3.03 (1 day) CLEAN-2002 : Working in a cleanroom : 2.4.03 (half-day, afternoon, free course, registration required) LabView base 1/LabView Basics 1 : 9 - 11.4.03 (3 jours/3 days) Langue à définir/language to be decided DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03 (6 jours) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 11 & 12.3.03 / 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 31.3 - 4.4.03 / 16...

  9. Places available**

    CERN Document Server

    2003-01-01

    If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses : EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) CLEAN-2002 : Working in a Cleanroom (free of charge) : 23.10.03 (half day) The EDMS-MTF in practice (free of charge) :  28 -  30.10.03 (6 half-day sessions) AutoCAD 2002 - Level 1 : 3, 4, 12, 13.11.03 (4 days) LabVIEW TestStand ver. 3 : 4 & 5.11.03 (2 days) Introduction to Pspice : 4.11.03 p.m. (half-day) Hands-on Introduction to Python Programm...

  10. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: Conception de PCB rapides dans le flot Cadence : 11.6.03 (matin) EXCEL 2000 - level 1 : 12 & 13.6.03 (2 days) Introduction to PVSS : 16.6.03 (p.m.) Basic PVSS : 17 - 19.6.03 (3 days) Réalisation de PCB rapides dans le flot Cadence : 17.6.03 (matin) PVSS - JCOP Framework Tutorial : 20.6.03 (1 day) Programmation automate Schneider : Programmation automate Schneider TSX Premium - 2ème niveau : 24 - 27.6.03 (4 jours) - audience : toute personne qui veux maitriser la mise en uvre et la programmation des fonctions spécialisées d'un automate TSX Premium - objectifs : maitriser la mise en uvre et la programmation des fonctions spécialisées d'un automate TSX Premium Cours de sécurité : Etre TSO au CERN : Prochaines sessions : 24, 25 & 27.6.03 - 4, 5 & 7.11.03 (session de 3 jours) ** The number of places available may vary. Please check our Web site to find out the current availability. If you wish to participate in one of these courses, pl...

  11. Places available **

    CERN Multimedia

    2003-01-01

    Des places sont disponibles dans les cours suivants : Places are available in the following course : Introduction to the CERN Engineering Data Management System : 28.1.03 (1 day) AutoCAD 2002 - niveau 1 : 24, 25.2 et 3, 4.3.03 (4 jours) AutoCAD 2002 - niveau 2 : 27 & 28.2.03 (2 jours) C++ for Particle Physicists : 10 - 14.3.03 (6 X 3 hour lectures) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 17, 18, 24 & 25.3.03 (6 jours) CLEAN-2002 : Working in a cleanroom : 25.3.03 (half-day, afternoon, free course, registration required) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 11 & 12.3.03 / 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 31.3 - 4.4.03 / 16 - 20.6.03 (5 jours/5 days) Réseau Simatic Net /Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 Ces cours seront donnés en français ou anglais en fonction des demandes / These courses will be given in French or English following the requests. * Etant do...

  12. Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: MATLAB Fundamentals and Programming Techniques (ML01) :2 & 3.12.03 (2 days) Oracle 8i : SQL : 3 - 5.12.03 (3 days) The EDMS MTF in practice : 5.12.03 (afternoon, free of charge) Modeling Dynamic Systems with Simulink (SL01) : 8 & 9.12.03 (2 days) Signal Processing with MATLAB (SG01) : 11 & ...

  13. Places available**

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The JAVA Programming Language Level 1 : 9 & 10.1.2004 (2 days) The JAVA Programming Language Level 2 : 11 to 13.1.2004 (3 days) LabVIEW base 1 : 25 - 27.2.2004 (3 jours) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon - free of charge) C++ for Particle Physicists : 8 - 12.3.2004 ( 6 X 4-hour sessions) LabVIEW Basics 1 : 22 - 24.3.20...

  14. Places available**

    CERN Document Server

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The JAVA Programming Language Level 1 :9 & 10.1.2004 (2 days) The JAVA Programming Language Level 2 : 11 to 13.1.2004 (3 days) Hands-on Introduction to Python Programming : 16 - 18.2.2004 (3 days - free of charge) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon - free of charge) C++ for Particle Physicists : 8 - 12.3.2004...

  15. Places available**

    CERN Document Server

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval Tel. 74924technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: MATLAB Fundamentals and Programming Techniques (ML01) : 2 & 3.12.03 (2 days) Oracle 8i : SQL : 3 - 5.12.03 (3 days) The EDMS MTF in practice : 5.12.03 (afternoon, free of charge) Modeling Dynamic Systems with Simulink (SL01) : 8 & 9.12.03 (2 days) Signal Processing with MATLAB (SG01) : 11 & 12.12.03 (2 days) The JAVA Programming Language - l...

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    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval Tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: JAVA 2 Enterprise Edition - Part 1 : WEB Applications : 20 & 21.11.03(2 days) FrontPage 2000 - niveau 1 : 20 & 21.11.03 (2 jours) Oracle 8i : SQL : 3 - 5.12.03 (3 days) Oracle 8i : Programming with PL/SQL : 8 - 10.12.03 (3 days) The JAVA Programming Language - leve...

  18. EVALUATION OF SUBSTRATES AND AMF SPORULATION IN THE PRODUCTION OF SEEDLINGS OF NATIVE FOREST SPECIES

    Directory of Open Access Journals (Sweden)

    Luciana de Moura Gonzaga

    2016-04-01

    Full Text Available ABSTRACT The objective of this study was to evaluate organic substrates in the production of canafistula (Peltophorum dubium (Spreng. Taub, cutieira (Joannesiaprinceps Vell., jatoba (Hymenaea courbaril L. and rubber tree (Hevea brasiliensis M. Arg. seedlings, native trees with potential use in forest restoration programs. The design was completely randomized with 10 substrate formulations with 4 repetitions of 3 plants for the four species. The evaluated substrates consisted of soil, bovine manure (BM, poultry manure (PM, chemical fertilizer (CF and sand, in different proportions. The experiment was concluded at the end of 180 days for canafistula, cutieira and rubber and 210 days for jatoba. At the end of these periods, the root (RDM, shoot (SDM and total (TDM the dry matters of the seedlings were determined. Quantification of AMF spores and normalization between samples through SPORES/RDM correction were also performed. The Scott-Knott test at 5% probability was applied. Regarding biomass production, only canafistula had significant difference among the tested substrates. In relation to sporulation, the highest values were observed in cutieira and rubber tree in substrate containing PM. The substrates composed of 40 or 50% soil + 20% sand + 30% or 40 PM for canafistula; 50% soil + 20% sand + 30% PM for cutieira; and for jatoba and rubber tree 60% soil + 20% sand + 20% PM, enabled the best results in terms of biomass production in seedlings and AMF sporulation.

  19. Functional analysis of the sporulation-specific diadenylate cyclase CdaS in Bacillus thuringiensis

    Science.gov (United States)

    Zheng, Cao; Ma, Yang; Wang, Xun; Xie, Yuqun; Ali, Maria K.; He, Jin

    2015-01-01

    Cyclic di-AMP (c-di-AMP) is a recently discovered bacterial secondary messenger molecule, which is associated with various physiological functions. In the genus Bacillus, the intracellular level and turnover of c-di-AMP are mainly regulated by three diadenylate cyclases (DACs), including DisA, CdaA and CdaS, and two c-di-AMP-specific phosphodiesterases (GdpP and PgpH). In this study, we demonstrated that CdaS protein from B. thuringiensis is a hexameric DAC protein that can convert ATP or ADP to c-di-AMP in vitro and the N-terminal YojJ domain is essential for the DAC activity. Based on the markerless gene knock-out method, we demonstrated that the transcription of cdaS was initiated by the sporulation-specific sigma factor σH and the deletion of cdaS significantly delayed sporulation and parasporal crystal formation. These findings contrast with similar experiments conducted using B. subtilis, wherein transcription of its cdaS was initiated by the sigma factor σG. Deletion of all the three DAC genes from a single strain was unsuccessful, suggesting that c-di-AMP is an indispensable molecule in B. thuringiensis. Phylogenetic analysis indicated increased diversity of CdaS in the B. cereus and B. subtilis Bacillus subgroups. In summary, this study identifies important aspects in the regulation of c-di-AMP in the genus Bacillus. PMID:26441857

  20. Maintenance and assessment of cell viability in formulation of non-sporulating bacterial inoculants.

    Science.gov (United States)

    Berninger, Teresa; González López, Óscar; Bejarano, Ana; Preininger, Claudia; Sessitsch, Angela

    2018-03-01

    The application of beneficial, plant-associated microorganisms is a sustainable approach to improving crop performance in agriculture. However, microbial inoculants are often susceptible to prolonged periods of storage and deleterious environmental factors, which negatively impact their viability and ultimately limit efficacy in the field. This particularly concerns non-sporulating bacteria. To overcome this challenge, the availability of protective formulations is crucial. Numerous parameters influence the viability of microbial cells, with drying procedures generally being among the most critical ones. Thus, technological advances to attenuate the desiccation stress imposed on living cells are key to successful formulation development. In this review, we discuss the core aspects important to consider when aiming at high cell viability of non-sporulating bacteria to be applied as microbial inoculants in agriculture. We elaborate the suitability of commonly applied drying methods (freeze-drying, vacuum-drying, spray-drying, fluidized bed-drying, air-drying) and potential measures to prevent cell damage from desiccation (externally applied protectants, stress pre-conditioning, triggering of exopolysaccharide secretion, 'helper' strains). Furthermore, we point out methods for assessing bacterial viability, such as colony counting, spectrophotometry, microcalorimetry, flow cytometry and viability qPCR. Choosing appropriate technologies for maintenance of cell viability and evaluation thereof will render formulation development more efficient. This in turn will aid in utilizing the vast potential of promising, plant beneficial bacteria as sustainable alternatives to standard agrochemicals. © 2018 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

  1. clpC operon regulates cell architecture and sporulation in Bacillus anthracis.

    Science.gov (United States)

    Singh, Lalit K; Dhasmana, Neha; Sajid, Andaleeb; Kumar, Prasun; Bhaduri, Asani; Bharadwaj, Mitasha; Gandotra, Sheetal; Kalia, Vipin C; Das, Taposh K; Goel, Ajay K; Pomerantsev, Andrei P; Misra, Richa; Gerth, Ulf; Leppla, Stephen H; Singh, Yogendra

    2015-03-01

    The clpC operon is known to regulate several processes such as genetic competence, protein degradation and stress survival in bacteria. Here, we describe the role of clpC operon in Bacillus anthracis. We generated knockout strains of the clpC operon genes to investigate the impact of CtsR, McsA, McsB and ClpC deletion on essential processes of B. anthracis. We observed that growth, cell division, sporulation and germination were severely affected in mcsB and clpC deleted strains, while none of deletions affected toxin secretion. Growth defect in these strains was pronounced at elevated temperature. The growth pattern gets restored on complementation of mcsB and clpC in respective mutants. Electron microscopic examination revealed that mcsB and clpC deletion also causes defect in septum formation leading to cell elongation. These vegetative cell deformities were accompanied by inability of mutant strains to generate morphologically intact spores. Higher levels of polyhydroxybutyrate granules accumulation were also observed in these deletion strains, indicating a defect in sporulation process. Our results demonstrate, for the first time, the vital role played by McsB and ClpC in physiology of B. anthracis and open up further interest on this operon, which might be of importance to success of B. anthracis as pathogen. © 2014 Society for Applied Microbiology and John Wiley & Sons Ltd.

  2. Asexual sporulation signalling regulates autolysis of Aspergillus nidulans via modulating the chitinase ChiB production.

    Science.gov (United States)

    Pócsi, I; Leiter, E; Kwon, N-J; Shin, K-S; Kwon, G-S; Pusztahelyi, T; Emri, T; Abuknesha, R A; Price, R G; Yu, J-H

    2009-08-01

    Elucidation of the regulation of ChiB production in Aspergillus nidulans. Mutational inactivation of the A. nidulans chiB gene resulted in a nonautolytic phenotype. To better understand the mechanisms controlling both developmental progression and fungal autolysis, we examined a range of autolysis-associated parameters in A. nidulans developmental and/or autolytic mutants. Investigation of disorganization of mycelial pellets, loss of biomass, extra-/intracellular chitinase activities, ChiB production and chiB mRNA levels in various cultures revealed that, in submerged cultures, initialization of autolysis and stationary phase-induced ChiB production are intimately coupled, and that both processes are controlled by the FluG-BrlA asexual sporulation regulatory pathway. ChiB production does not affect the progression of apoptotic cell death in the aging A. nidulans cultures. The endochitinase ChiB plays an important role in autolysis of A. nidulans, and its production is initiated by FluG-BrlA signalling. Despite the fact that apoptosis is an inseparable part of fungal autolysis, its regulation is independent to FluG-initiated sporulation signalling. Deletion of chiB and fluG homologues in industrial filamentous fungal strains may stabilize the hyphal structures in the autolytic phase of growth and limit the release of autolytic hydrolases into the culture medium.

  3. PLACES AVAILABLE

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    Places are available in the following courses: Utilisation du simulateur Simplorer : 30.5 - 1.6.01 (3 jours) Contract Follow-up : 11.6.01 (1/2 journée) LabView hands-on : 11.6.01 F ou E (1/2 journée) LabView Base 1 : 12 - 14.6.01 (3 jours) Habilitation électrique : superviseurs: 2 sessions d'une demi-journée les 12 et 19.6.01 If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

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    Places are available in the following courses: The JAVA programming language level 1: 8 - 9.2.01 (2 days) AutoCAD 2D niveau 1 : 12 - 16.2.01 (5 jours) The JAVA programming language level 2: 19 - 21.2.01 (3 days) C++ for Particle Physicists: 5 - 9.3.01 (20 hrs on 5 days) Contract Follow-up : 12.3.01 (3 heures) The JAVA programming language level 2: 12 - 14.3.01 (3 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

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    Places are available in the following courses: December 2002   PCAD Schémas - Débutants :  5 & 6.12.02  (2 jours) PCAD PCB - Débutants :  9 - 11.12.02  (3 jours) FrontPage 2000 - level 1:  9 & 10.12.02  (2 days) Introduction à la CAO Cadence (cours gratuit) :  10 & 11.12.02  (2 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval Tel.74924 monique.duval@cern.ch

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    Places are available in the following courses: CLEAN-2002 : Travailler en salle blanche (cours gratuit) : 13.08.2002 (matin) Introduction to the CERN Enginnering Data Management System :  27.8.02  (1 day) The CERN Engineering Data Management System for Advanced Users :  28.8.02  (1 day) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval Tel.74924 monique.duval@cern.ch    

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    Places are available in the following courses: C++ Programming Level 2 - Traps & Pitfalls:  16 - 19.7.02 (4 days) Frontpage 2000 - level 1 :  22 - 23.7.02  (2 days) Introduction à Windows 2000 au CERN : 24.7.02 (après-midi) CLEAN-2002 : Travailler en salle blanche (cours gratuit) : 13.08.2002 (matin) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval Tel.74924 monique.duval@cern.ch

  8. Placing knowledge

    DEFF Research Database (Denmark)

    Adriansen, Hanne Kirstine; Valentin, Karen; Nielsen, Gritt B.

    Internationalisation of higher education is premised by a seeming paradox: On the one hand, academic knowledge strives to be universal in the sense that it claims to produce generalizable, valid and reliable knowledge that can be used, critiqued, and redeveloped by academics from all over the world......; on the other hand, the rationale for strengthening mobility through internationalisation is based on an imagination of the potentials of particular locations (academic institutions). Intrigued by this tension between universality and particularity in academic knowledge production, this paper presents...... preliminary findings from a project that study internationalisation of higher education as an agent in the interrelated processes of place-making and knowledge-making. The project is based on three case-studies. In this paper, focus is on PhD students’ change of research environment. This is used as a case...

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    Places are available in the following courses: Introduction to Perl 5 : 2 - 3.7.01 (2 days) Introduction to Databases :  3 - 4.7.01 (2 days) JAVA programming language Level 2 : 4 - 6.7.01 (3 days) Enterprise JavaBeans :  9 - 11.7.01 (3 days) Design Patterns :  10 - 12.7.01 (3 days) C++ for Particle Physicists :  23 - 27.7.01 (6 3-hour lectures) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

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    Places are available in the following courses: The Java programming language (Level 1) : 8 - 9.2.01 (2 days) Architecture d'automatisme : 20 - 21.2.01 (2 jours) Programmation TSX Premium 1 (Schneider) : 26.2 - 2.3.01 (5 jours) C++ for Particle Physicists : 5 - 9.3.01 (6*3 hour lectures) The Java programming language (Level 2) : 12 - 14.3.2001 (3 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

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    Places are available in the following courses: Introduction to Databases :  3 - 4.7.01 (2 days) The JAVA programming language Level 2 : 4 - 6.7.01 (3 days) Enterprise JavaBeans :  9 - 11.7.01 (3 days) Design Patterns :  10 - 12.7.01 (3 days) C++ for Particle Physicists :  23 - 27.7.01 (6 3-hour lectures) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

  12. Growth and Sporulation of Bacillus cereus ATCC 14579 under Defined Conditions: Temporal Expression of Genes for Key Sigma Factors

    OpenAIRE

    de Vries, Ynte P.; Hornstra, Luc M.; de Vos, Willem M.; Abee, Tjakko

    2004-01-01

    An airlift fermentor system allowing precise regulation of pH and aeration combined with a chemically defined medium was used to study growth and sporulation of Bacillus cereus ATCC 14579. Sporulation was complete and synchronous. Expression of sigA, sigB, sigF, and sigG was monitored with real-time reverse transcription-PCR, and the pattern qualitatively resembled that of Bacillus subtilis. This method allows reproducible production of stable spores, while the synchronous growth and defined ...

  13. Growth and sporulation of Bacillus cereus ATCC 14579 under defined conditions: temporal expression of genes for key sigma factors.

    Science.gov (United States)

    de Vries, Ynte P; Hornstra, Luc M; de Vos, Willem M; Abee, Tjakko

    2004-04-01

    An airlift fermentor system allowing precise regulation of pH and aeration combined with a chemically defined medium was used to study growth and sporulation of Bacillus cereus ATCC 14579. Sporulation was complete and synchronous. Expression of sigA, sigB, sigF, and sigG was monitored with real-time reverse transcription-PCR, and the pattern qualitatively resembled that of Bacillus subtilis. This method allows reproducible production of stable spores, while the synchronous growth and defined conditions are excellently suitable for further gene expression studies of cellular differentiation of B. cereus.

  14. ‘We are a community [but] that takes a certain amount of energy’: Exploring shared visions, social action, and resilience in place-based community-led energy initiatives

    International Nuclear Information System (INIS)

    Parkhill, K.A.; Shirani, F.; Butler, C.; Henwood, K.L.; Groves, C.; Pidgeon, N.F.

    2015-01-01

    Highlights: • We engage with conceptual characteristics of 3 community-led energy case studies. • We examine data from interviews to explore the issues community energy groups face. • Shared visions, social action and social resilience are important to community energy. • Creating and maintaining shared visions, social action and social resilience is extremely challenging. - Abstract: In UK energy policy, community-led energy initiatives are increasingly being imbued with transformative power to facilitate low carbon transitions. The ways that such expectations for communities are manifesting in practice remains, however, relatively poorly understood. In particular, key conceptual developments in unpacking what constitutes ‘community’ that highlight the significance of ‘place’ along with important characteristics, such as shared visions, collective social action, and resilience, have yet to be comprehensively explored in the context of community-led energy initiatives. This paper uses an interpretive stance to engage with these conceptual ideas about community and provides insights into the nature of community and its meaning for developing energy-related initiatives and realising the wider goals of energy policy. The paper draws on data from in-depth qualitative, longitudinal interviews undertaken in two residential communities and one purely workplace-based community, which are engaged in community energy initiatives. We argue that there are difficulties and ambiguities in creating shared visions, achieving social action, and developing resilience that are related to the specificities of community in place, but that all three characteristics are likely to be important for the making of sustainable places

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    Des places sont disponibles dans les cours suivants : Places are available in the following course : WorldFIP 2003 pour utilisateurs : 11 - 14.2.03 (4 jours) AutoCAD 2002 - niveau 1 : 24, 25.2 & 3, 4.3.03 (4 jours) Introduction à Windows 2000 au CERN : 25.2.03 (1/2 journée) AutoCAD 2002 - niveau 2 : 27 & 28.2.03 (2 jours) C++ for Particle Physicists : 10 - 14.3.03 (6 X 3 hour lectures) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 17, 18, 24 & 25.3.03 (6 jours) CLEAN-2002 : Working in a cleanroom : 2.4.03 (half-day, afternoon, free course, registration required) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 11 & 12.3.03 / 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 31.3 - 4.4.03 / 16 - 20.6.03 (5 jours/5 days) Réseau Simatic Net /Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 Ces cours seront donnés en français ou anglais en fonction des demandes / These courses will be given in French o...

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    Places are available in the following courses: LabVIEW base 1 : 22 - 24.4.02 (3 jours) CLEAN 2002 : working in a cleanroom:  24.4.02  (half-day, pm) LabVIEW DSC (F) 25 & 26.4.02 (2 jours) AutoCAD : Mise à jour AutoCAD r-14 vers 2002 : 25 & 26.4.02 (2 jours) Cotations selon les normes GPS de l'ISO : 29 - 30.4.02 (2 jours) Introduction to the CERN Engineering Data Management System:  7.5.02  (1 day) LabVIEW Basics 2: 13 & 14.5.02 (2 days) AutoCAD Mechanical 6 PowerPack (F) : 13-14, 17, 21, 27-28.5.02 (6 jours) WorldFIP - Généralités : 14.5.2002 (1/2 journée) WorldFIP - Développer avec MicroFIP HANDLER : 14.5 - après-midi, 15.5.02 - matin (1 jour) WorldFIP - FullFIP FDM : FIP Device Manager (F) : 15.5 - après-midi, 16.5.02 - matin (1 jour) LabVIEW DAQ (F) : 15 & 16.5.02 (2 jours) EXCEL 2000 - niveau 2 : 22 & 23.5.02 (2 jours)...

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    Places are available in the following courses : CLEAN-2002 : Working in a cleanroom (free course, registration required) : 2.4.03 (half-day, afternoon) LabView base 1/LabView Basics 1 (Langue à définir/ language to be decided) : 9 - 11.4.03 (3 jours/3 days) DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03(6 jours) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 31.3 - 4.4.03 / 16 - 20.6.03 (5 jours/5 days) Réseau Simatic Net /Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 These courses will be given in French or English following the requests. Cours de sécurité : Etre TSO au CERN : 3 sessions sont programmées pour 2003 : 25...

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    Places are available in the following courses : CLEAN-2002 : Working in a cleanroom (free course, registration required): 11.4.03 (half-day, afternoon, ) LabView Basics 2 : 10 - 11.4.03 (3 days) DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) Oracle iDS Reports : Build Internet Reports : 5 - 9.5.03 (5 days) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03 (6 jours) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 31.3 - 4.4.03 / 16 - 20.6.03 (5 jours/5 days) Réseau Simatic Net /Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 These courses will be given in French or English following the requests. Cours de sécurité : Etre TSO au CERN : Prochaines sessions : 24, 25 & 27.6....

  1. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) Oracle iDS Reports : Build Internet Reports : 5 - 9.5.03 (5 days) LabView DAQ (language to be defined) : 8 & 9.5.03 AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03 (6 jours) AutoCAD 2002 - niveau 2 : 3 & 4.6.03 (2 jours) LabView DSC (language to be defined) : 19 & 20.6.03 Siemens SIMATIC Training : Introduction to STEP7 : 3 & 4.6.03 (2 days) STEP7 Programming : 16 - 20.6.03 (5 days) Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 (sessions of 2 days) These courses will be given in French or English following the requests. Cours de sécurité : Etre TSO au CERN : Prochaines sessions : 24, 25 & 27.6.03 - 4, 5 & 7.11.03 (session de 3 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description ...

  2. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Habilitation électrique : recyclage HT/BT : 11 - 15.3.2002  (2 * 2 heures) PVSS Basics :  8 - 12.4.02  (5 days) ELEC-2002 : Spring Term :  9, 11, 16, 18, 23, 25, 30.4.02 (7 * 2.5 hours) LabVIEW base 1 : 22 - 24.4.02 (3 jours) LabVIEW DSC (F) 25 & 26.4.02 (2 jours) LabVIEW Basics 2 : 13 & 14.5.02 (2 days) LabVIEW DAQ (F) : 15 & 16.5.02 (2 jours) Cours sur la migration AutoCAD :   AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé sur AutoCAD 2002 (5 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applica...

  3. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : CLEAN-2002 : Working in a cleanroom (free course, registration required): 11.4.03 (half-day, afternoon, ) LabView Basics 2 : 10 - 11.4.03 (3 days) DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) Oracle iDS Reports : Build Internet Reports : 5 - 9.5.03 (5 days) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03(6 jours) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 31.3 - 4.4.03 / 16 - 20.6.03 (5 jours/5 days) Réseau Simatic Net /Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 These courses will be given in French or English following the requests. Cours de sécurité : Etre TSO au CERN : Prochaines sessions : 24, 25 & 27.6...

  4. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) Programmation de pilotes périphériques : 5 - 8.5.03 (4 jours) Oracle iDS Reports : Build Internet Reports : 5 - 9.5.03 (5 days) LabView DAQ (language to be defined) : 8 & 9.5.03 AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.0 (6 jours) FrontPage 2000 - niveau 1 : 20 & 21.5.03 (2 jours) AutoCAD 2002 - niveau 2 : 3 & 4.6.03 (2 jours) EXCEL 2000 - niveau 1 : 10 & 11.6.03 (2 jours) EXCEL 2000 - level 1 : 12 & 13.6.03 (2 days) PowerPoint 2000 (F) : 17 & 18.6.03 (2 jours) FrontPage 2000 - niveau 2 : 19 & 20.6.03 (2 jours) LabView DSC (langue à décider/language to be defined) : 19 & 20.6.03 EXCEL 2000 - niveau 2 : 24 & 25.6.03 (2 jours) Siemens SIMATIC Training : Introduction to STEP7 : 3 & 4.6.03 (2 days) STEP7 Programming : 16 - 20.6.03 (5 days) Simatic Net Network : 26 & 27.6.03 ...

  5. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : CLEAN-2002 : Working in a cleanroom (free course, registration required): 11.4.03 (half-day, afternoon) LabView Basics 2 : 10 - 11.4.03 (3 days) DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) Oracle iDS Reports : Build Internet Reports : 5 - 9.5.03 (5 days) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03(6 jours) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 16 - 20.6.03 (5 jours/5 days) Réseau Simatic Net /Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 These courses will be given in French or English following the requests. Cours de sécurité : Etre TSO au CERN : Prochaines sessions : 24, 25 & 27.6.03 - 4, 5 & 7....

  6. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : Introduction to PVSS : 10.3.03 (half-day, afternoon) CLEAN-2002 : Working in a cleanroom : 2.4.03 (half-day, afternoon, free course, registration required) LabView Basics 1 : 9 - 11.4.03 (3 days) Language to be decided. DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures). AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03 (6 jours) Siemens SIMATIC Training: Introduction to STEP7 : 3 & 4.6.03 (2 days) STEP7 Programming : 31.3 - 4.4.03 / 16 - 20.6.03 (5 days) Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 These courses will be given in French or English following the requests. Cours de sécurité: Etre TSO au CERN : 3 sessions sont programmées pour 2003 : 25, 26 & 28.3.03 - 24, 25 & 27.6.03 - 4, 5 & 7.11.03 (sessions de 3 jours) ** The number o...

  7. PLACES AVAILABLE

    CERN Document Server

    Monique Duval

    2002-01-01

    Places are available in the following courses: LabVIEW Basics 2: 13 & 14.5.02 (2 days) WorldFIP - Généralités : 14.5.2002 (1/2 journée) WorldFIP - Développer avec MicroFIP HANDLER : 14.5 - après-midi, 15.5.02 - matin (1 jour) WorldFIP - FullFIP FDM : FIP Device Manager (F) : 15.5 - après-midi, 16.5.02 - matin (1 jour) LabVIEW DAQ (F) : 15 & 16.5.02 (2 jours) EXCEL 2000 - niveau 2 : 22 & 23.5.02 (2 jours) The CERN Engineering Data Management System for Advanced users:  30.5.02  (1 day) LabVIEW Basics 1:  3 - 5.6.02  (3 days) AutoCAD 2002 - condensé : 4 - 6.6.02 (3 jours) LabVIEW DAQ (E):  6 & 7.6.02  (2 days) AutoCAD 2002 - Level 1:  10 - 12 and 24 - 26.6.02  (6 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the c...

  8. PLACES AVAILABLE

    CERN Document Server

    Monique Duval

    2002-01-01

    Places are available in the following courses: Introduction to the CERN Engineering Data Management System:  7.5.02  (1 day) LabVIEW Basics 2: 13 & 14.5.02 (2 days) AutoCAD Mechanical 6 PowerPack (F) : 13-14, 17, 21, 27-28.5.02 (6 jours) WorldFIP - Généralités : 14.5.2002 (1/2 journée) WorldFIP - Développer avec MicroFIP HANDLER : 14.5 - après-midi, 15.5.02 - matin (1 jour) WorldFIP - FullFIP FDM : FIP Device Manager (F) : 15.5 - après-midi, 16.5.02 - matin (1 jour) LabVIEW DAQ (F) : 15 & 16.5.02 (2 jours) EXCEL 2000 - niveau 2 : 22 & 23.5.02 (2 jours) The CERN Engineering Data Management System for Advanced users:  30.5.02  (1 day) LabVIEW Basics 1:  3 - 5.6.02  (3 days) AutoCAD 2002 - condensé : 4 - 6.6.02 (3 jours) LabVIEW DAQ (E):  6 & 7.6.02  (2 days) AutoCAD 2002 - Level 1:  10 - ...

  9. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: ELEC-2002 : Spring Term :  9, 11, 16, 18, 23, 25, 30.4.02 (7 * 2.5 hours) Object-Oriented Analysis & Design: 16 - 19.4.02  (4 days) The CERN Engineering Data Management System for Advanced users:  16.4.02  (1 day) Migration from AutoCAD 14 towards AutoCAD Mechanical6 PowerPack:  17 - 19.4 and 2 &3.5.02  (5 days) AutoCAD - niveau 1 : 22, 23, 29, 30.4 et 6, 7.5.02 (6 jours) LabVIEW base 1 : 22 - 24.4.02 (3 jours) CLEAN 2002 : working in a cleanroom:  24.4.02  (half-day, pm) LabVIEW DSC (F) 25 & 26.4.02 (2 jours) AutoCAD : Mise à jour AutoCAD r-14 vers 2002 : 25 & 26.4.02 (2 jours) LabVIEW Basics 2 : 13 & 14.5.02 (2 days) EXCEL 2000 - niveau 1 : 15 & 16.5.02 (2 jours) LabVIEW DAQ (F) : 15 & 16.5.02 (2 jours) EXCEL 2000 - niveau 2 : 22 & 23.5.02 (2 jours) LabVIEW Basics 1:  3 - 5.6.02&a...

  10. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel.74924

    2001-01-01

    Places are available in the following courses: Habilitation électrique : superviseurs : 5.12.01 (1/2 journée) LabVIEW - Basics 1 :  10 - 12.12.01 (3 days) Introduction au PC et Windows 2000 : 12 & 14.12.01 (2 jours) LabVIEW - Basics 2 :  13 - 14.12.01 (2 days) Habilitation électrique : superviseurs : 17.12.2001 (1/2 journée) EXCEL 2000 - niveau 2 : 15 - 16.1.02 (2 jours) Sécurité dans les installations cryogéniques: 15-17.1.2002 (2 demi-journées) C++ Programming Level 2 - Traps and Pitfalls :  15 - 18.1.2002  (4 days) ELEC-2002 Winter Term: Readout and system electronics for Physics  15.1.2002 - 7.2.2002 (8 half- days) Nouveautés de WORD 2000 : 18.1.02 (1/2 journée) LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 - 25.1.02 (4 jours) Frontpage...

  11. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: C++ for Particle Physicists :  11 - 15.3.2002  (6 * 3 hour lectures) Programming the Web for Control Applications : 11, 12, 18, 19.3.2002  (4 * 2 hour lectures) Habilitation électrique : recyclage HT/BT (Français) : 13 - 14.3.2002 (2 * 2 heures) Introduction à la CAO CADENCE : 19 & 20.3.02 (2 jours) LabVIEW base 1 : 22 - 24.4.02 (3 jours) LabVIEW DSC (F) 25 & 26.4.02 (2 jours) LabVIEW Basics 2 : 13 & 14.5.02 (2 days) LabVIEW DAQ (F) : 15 & 16.5.02 (2 jours) Cours sur la migration AutoCAD :   AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé sur AutoCAD 2002 (5 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fil...

  12. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Introduction au PC et à Windows 2000 au CERN : 29 - 30.1.02 (2 jours) LabView Base 1 : 4 - 6.2.02 (3 jours) LabView DAQ  (F) : 7 & 8.2.02 (2 jours) Hands-on Object-Oriented Design & Programming with Java :  11 - 13.02.02 (3 days) PVSS basics :  18 - 22.2.02 (5 days) Introduction à Windows 2000 : 18.2.02 (1 demi-journée) Introduction to the CERN Engineering Data Management System :  20.2.02 (1 day) The CERN Engineering Data Management System for Advanced users :  21.2.02  (1 day) C++ for Particle Physicists :  11 - 15.3.2002  (6 * 3 hour lectures) Cours sur la migration AutoCAD : AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé sur AutoCAD 2002 (5 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electr...

  13. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: PVSS Basics :  8 - 12.4.02  (5 days) AutoCAD : Mise à jour AutoCAD r-14 vers 2002 : 25 & 26.4.02 (2 jours) ELEC-2002 : Spring Term :  9, 11, 16, 18, 23, 25, 30.4.02 (7 * 2.5 hours) Object-Oriented Analysis & Design: 16 - 19.4.02  (4 days) Migration from AutoCAD 14 towards AutoCAD Mechanical6 PowerPack:  17 - 19.4 and 2 &3.5.02  (5 days) LabVIEW base 1 : 22 - 24.4.02 (3 jours) LabVIEW DSC (F) 25 & 26.4.02 (2 jours) LabVIEW Basics 2 : 13 & 14.5.02 (2 days) EXCEL 2000 - niveau 2 : 22 & 23.5.02 (2 jours) LabVIEW DAQ (F) : 15 & 16.5.02 (2 jours) LabVIEW Basics 1:  3 - 5.6.02  (3 days) LabVIEW DAQ (E):  6 & 7.6.02  (2 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that...

  14. PLACES AVAILABLE

    CERN Multimedia

    Monique DUVAL

    2002-01-01

    Places are available in the following courses: LabView Basics 1 :  4 - 6.3.02  (3 days) CLEAN-2002 : Working in a Clean Room :  7.3.2002  (half day) LabView Base 2 : 11 & 12.3.02 (2 jours) C++ for Particle Physicists :  11 - 15.3.2002  (6 * 3 hour lectures) Programming the Web for Control Applications : 11, 12, 18, 19.3.2002  (4 * 2 hour lectures) Habilitation électrique : recyclage HT/BT (Français) : 13 - 14.3.2002 (2 * 2 heures) LabView Advanced :  13 - 15.3.02 (3 days) Introduction to the CERN Engineering Data Management System (EDMS) :  20.3.2002  (1 day) The CERN (EDMS) for Advanced Users :  21.3.2002  (1 day) LabVIEW DSC : 25 - 26.4.2002 (2 jours) LabVIEW DAQ : 15 - 16.5.2002 (2 jours) Cours sur la migration AutoCAD :   AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé ...

  15. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: PVSS Basics : 20 - 24.8.01 (5 days) PROFIBUS : 25 - 26.9.01 (2 jours) PROFIBUS : 27 - 28.9.01 (2 days) PCAD Schémas - débutants : 4 - 5.10.01 (2 jours) PCAD PCB - débutants : 8 - 10.10.01 (3 jours) Programming TSX Premium 1: 15 - 19.10.01 (5 days) Programmation TSX Premium 1 : 22 - 26.10.01 (5 jours) Programming TSX Premium 2: 19 - 23.11.01 (5 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) The following LabView courses will be given in either English or French according to demand LabVIEW - Base 1 / LabVIEW - Basics 1 : 10 - 12.9.01 (3 jours / 3 days) LabVIEW - DAQ / LabVIEW - DAQ : 13 - 14.9.01 (2 jours / 2 days) LabVIEW - Base 1 / LabVIEW - Basics 1 : 15 - 17.10.01 (3 jours / 3 days) LabVIEW - Base 2 / LabVIEW - Basics 2 : 18 - 19.10.01 (2 jours / 2 days) LabVIEW - Base 1 / LabVIEW - Basics 1 : 12 - 14.11.01 (3 jours / 3 days) LabVIEW - DAQ / LabVIEW - DAQ : 15 - 16.11.01 (2 jours / 2...

  16. PLACES AVAILABLE

    CERN Multimedia

    Technical training; Tel. 74924

    2001-01-01

    Places are available in the following courses: PROFIBUS : 25 - 26.9.01 (2 jours) PROFIBUS : 27 - 28.9.01 (2 days) Automates et réseaux de terrain : 3 - 4.10.2001 (2 jours) PCAD Schémas - débutants : 4 - 5.10.01 (2 jours) PCAD PCB - débutants : 8 - 10.10.01 (3 jours) Programmation TSX Premium 1 : 15 - 19.10.01 (5 jours) Programmation TSX Premium 1 : 22 - 26.10.01 (5 jours) Programming TSX Premium 2: 19 - 23.11.01 (5 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) Autocad Migration support courses: a detailed calendar will be published shortly for this series of sessions which will start on 15.10.2001. Registration is already open AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé sur AutoCAD 2002 (5 jours) The following LabView courses will be given in either English or French according to demand LabVIEW - Base 1 / LabVIEW - Basics 1 : 10 - 12.9.01 (3 jours / 3 days)...

  17. PLACES AVAILABLES

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: PVSS Basics : 20 - 24.8.01 (5 days) PROFIBUS : 25 - 26.9.01 (2 jours) PROFIBUS : 27 - 28.9.01 (2 days) PCAD Schémas - débutants : 4 - 5.10.01 (2 jours) PCAD PCB - débutants : 8 - 10.10.01 (3 jours) Programming TSX Premium 1: 15 - 19.10.01 (5 days) Programmation TSX Premium 1 : 22 - 26.10.01 (5 jours) Programming TSX Premium 2: 19 - 23.11.01 (5 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) The following LabView courses will be given in either English or French according to demand LabVIEW - Base 1 / LabVIEW - Basics 1 : 10 - 12.9.01 (3 jours / 3 days) LabVIEW - DAQ / LabVIEW - DAQ : 13 - 14.9.01 (2 jours / 2 days) LabVIEW - Base 1 / LabVIEW - Basics 1 : 15 - 17.10.01 (3 jours / 3 days) LabVIEW - Base 2 / LabVIEW - Basics 2 : 18 - 19.10.01 (2 jours / 2 days) LabVIEW - Base 1 / LabVIEW - Basics 1 : 12 - 14.11.01 (3 jours / 3 days) LabVIEW - DAQ / LabVIEW - DAQ : 15 - 16.11.01 (2 jours / 2...

  18. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: PVSS basics :  18 - 22.2.02 (5 days) Introduction à la CAO CADENCE : 20 & 21.2.02 (2 jours) LabView Basics 1 :  4 - 6.3.02  (3 days) Introduction au VHDL et utilisation du simulateur de CADENCE : 6 & 7.3.02 (2 jours) LabView Base 2 : 11 & 12.3.02 (2 jours) C++ for Particle Physicists :  11 - 15.3.2002  (6 * 3 hour lectures) LabView Advanced :  13 - 15.3.02 (3 days) Cours sur la migration AutoCAD :   AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé sur AutoCAD 2002 (5 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO...

  19. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: LabView DAQ  (F) : 7 & 8.2.02 (2 jours) Hands-on Object-Oriented Design & Programming with Java :  11 - 13.02.02 (3 days) PVSS basics :  18 - 22.2.02 (5 days) Introduction à Windows 2000 : 18.2.02 (1 demi-journée) Introduction to the CERN Engineering Data Management System :  20.2.02 (1 day) Introduction à la CAO CADENCE : 20 & 21.2.02 (2 jours) The CERN Engineering Data Management System for Advanced users :  21.2.02  (1 day) LabView Basics 1 :  4 - 6.3.02  (3 days) Introduction au VHDL et utilisation du simulateur de CADENCE : 6 & 7.3.02 (2 jours) LabView Base 2 : 11 & 12.3.02 (2 jours) C++ for Particle Physicists :  11 - 15.3.2002  (6 * 3 hour lectures) LabView Advanced :  13 - 15.3.02 (3 days) Cours sur la migration AutoCAD :   AutoCAD : Mise à...

  20. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Introduction à la CAO CADENCE : 20 & 21.2.02 (2 jours) LabView Basics 1 :  4 - 6.3.02  (3 days) Introduction au VHDL et utilisation du simulateur de CADENCE : 6 & 7.3.02 (2 jours) CLEAN-2002 : Working in a Clean Room :  7.3.2002  (1 day) LabView Base 2 : 11 & 12.3.02 (2 jours) C++ for Particle Physicists :  11 - 15.3.2002  (6 * 3 hour lectures) LabView Advanced :  13 - 15.3.02 (3 days) Cours sur la migration AutoCAD :   AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé sur AutoCAD 2002 (5 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisiona...

  1. Media places

    DEFF Research Database (Denmark)

    Linde, Per; Messeter, Jörn

    The impact that ubiquitous wireless network technologies and mobile phones have on our experience of the modern cityscape, has been a driving force in many research projects in recent years. The agendas differ in relation to perspectives, but it seems safe to claim that such technologies are no l......The impact that ubiquitous wireless network technologies and mobile phones have on our experience of the modern cityscape, has been a driving force in many research projects in recent years. The agendas differ in relation to perspectives, but it seems safe to claim that such technologies...... are no longer neutral layers in urban living, but rather an integrated part of the materialities of architecture and urban planning, the social dimensions of city life and emerging new cultural frameworks. Arguably, we have reached a point at which digital designs may be regarded as elements of our everyday...... of how meaning can emerge in the interplay between people, artifacts and place. This paper will present the early and ongoing work in the project “Media places”, which is part of the research platform for new media at Malmö University; MEDEA. The intention is to introduce conceptual foundations...

  2. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Introduction to C Programming :  21- 23.11.01 (3 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) Contract Follow-up (F) : 26.11.01 (1/2 journée) Habilitation électrique : électriciens network : 27 - 29.11.2001 (3 jours) Object-Oriented Analysis and Design :  27 - 30.11.2001  (4 days) Introduction to the CERN Engineering Data Management System :  30.11.2001 (1 day) Electromagnetic Compatibility (EMC): Introduction (bilingual) :  3.12.01 (half-day) Introduction to the CERN Engineering Data Management System : 07.12.2001 (1 day) LabVIEW - Basics 1 :  10 - 12.12.01 (3 days) LabVIEW - Basics 2 :  13 - 14.12.01 (2 days) EXCEL 2000 - niveau 2 : 15 - 16.1.02 (2 jours) C++ Programming Level 2 - Traps and Pitfalls :  15 - 18.1.2002  (4 days) Nouveautés de WORD 2000 : 18.1.02 (1/2 journée) FileMaker P...

  3. PLACES AVAILABLES

    CERN Multimedia

    Technical Training; Tel. 74924

    2000-01-01

    Places are available in the following courses:   C++ for Particle Physicists 20 - 24.11.00 6 lectures CANbus 20.11.00 1 journée CANopen 21 et 22.11.00 2 jours Sécutiré dans les installations cryogéniques 21 et 22.11.00 2 demi-journées The JAVA programming language level 2 27 ­ 29.11.00 3 days Contract Follow-up 27.11.00 3 heures 1/2 Cryogénie (introduction) 4 ­ 8.12.00 ANSYS Introduction : langue a décider suivant majorité 5 ­ 7.12.00 3 jours EXCEL 7, 8, 13 et 14.12.00 4 jours Contract Follow-up 15.12.00 3 heures 1/2 If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an “application for training” form available from your Divisional Secretariat or from your DTO (Divisional Training Officer)....

  4. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74460

    2001-01-01

    Places are available in the following courses: LabView Base 1 : 27-29.3.01 (3 jours) Contract Follow-up : 9.4.01 (3 heures) Introduction à PowerPoint : 24.4.01 (1 journée) Publier sur le Web : 25-27.4.01 (3 demi-journées) Programmation TSX Premium 2 : 15-16.5.01 (5 jours) LabView Base 2 : 27-29.3.01 (2 jours) Hands-on Object-oriented Analysis, Design & Programming with C++ :  23-27.4.01 (5 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

  5. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74460

    2001-01-01

    Places are available in the following courses: The JAVA programming language level 1 : 22 - 23.1.01 (2 days) Introduction to Databases : 23 - 24.1.01 (2 days) EXCEL : 24 - 25.1.01  et 1 - 2.2.01 (4 jours) Advanced and Modern Databases : 25 - 26.01.01 (2 days) UNIX pour non-programmeurs : 31.1 - 2.2.01 (3 jours) JAVA for non-programmers : 5 - 7.2.01 (3 days) Publier sur le Web :  6 - 8.2.01 (3 demi-journées) Contract Follow-up : 12.2.01 (3 heures) Introduction to Oracle SQL and PL/SQL : 12 - 16.2.01 (5 days) The JAVA programming language level 2 : 19 - 21.2.01 (3 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order...

  6. PLACES AVAILABLE

    CERN Multimedia

    TECHNICAL TRAINING; Tel. 74460

    2001-01-01

    Places are available in the following courses: Introduction to Databases : 23 - 24.1.01 (2 days) Advanced and Modern Databases : 25 - 26.01.01 (2 days) UNIX pour non-programmeurs : 31.1 - 2.2.01 (3 jours) JAVA for non-programmers : 5 - 7.2.01 (3 days) Contract Follow-up : 12.2.01 (3 heures) Introduction to Oracle SQL and PL/SQL : 12 - 16.2.01 (5 days) AutoCAD 2D niveau I : 12 - 16.2.02 (5 jours) The JAVA programming language level 2 : 19 - 21.2.2001 (3 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

  7. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Contract Follow-up (F) : 30.10.01 (1/2 journée) The CERN Engineering Data Management System for Electronics Design :  30.10.01 (1 day) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) Nouveautés d'EXCEL : 5.11.01 (1/2 journée) Introduction a Windows 2000 au CERN : 6.11.01 (1/2 journée) The Java programming language Level 1: 8 - 9.11.01 (2 days) LabView Base 1 : 12 - 14.11.01 (3 jours) Automates et réseaux de terrain : 13 & 14.11.01 (2 jours) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to XML :  19 - 20.11.01 (2 days) Programming TSX Premium 1 :  19 - 23.11.01  (5 days) Introduction to C Programming :  21- 23.11.01 (3 days) The Java programming language Level 2:  26 - 28.11.01 (3 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) Autocad Migration support courses: a detail...

  8. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Nouveautés d'EXCEL : 5.11.01 (1/2 journée) Introduction a Windows 2000 au CERN : 6.11.01 (1/2 journée) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) Design Patterns :  7 - 8.11.01 (2 days) The Java programming language Level 1: 8 - 9.11.01 (2 days) Automates et réseaux de terrain : 13 & 14.11.01 (3 jours) Introduction à Windows 2000 au CERN : 12 - 14.11.01 (1/2 journée) Introduction to Windows 2000 at CERN :  14.11.01  (half-day) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to C Programming :  21- 23.11.01 (3 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) Contract Follow-up (F) : 26.11.01 (1/2 journée) Object-Oriented Analysis and Design :  27 - 30.11.2001  (4 days) Hands-on Object-Oriented Design and Programming with C++ :  11 - 13.12.2...

  9. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Contract Follow-up (F) : 30.10.01 (1/2 journée) The CERN Engineering Data Management System for Electronics Design :  30.10.01 (1 day) Nouveautés d'Excel 2000 : 5.11.01 (1/2 journée) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) Introduction à Windows 2000 au CERN : 6.11.01 (1/2 journée) The Java programming language Level 1: 8 - 9.11.01 (2 days) LabView Base 1 : 12 - 14.11.01 (3 jours) LabVIEW DAQ (F) : 15 & 16.11.01 (2 jours) Automates et réseaux de terrain : 13 & 14.11.01 (2 jours) Introduction to PERL 5 :  15 - 16.11.01  (2 days) LabVIEW - DAQ : 15 - 16.11.01 (2 jours) Introduction to XML :  19 - 20.11.01 (2 days) Introduction to C Programming :  21- 23.11.01 (3 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) Object-Oriented Analysis and Design :  27 - 30.11.2001 (4 days) Hands...

  10. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Automates et réseaux de terrain : 13 & 14.11.01 (3 jours) Introduction à Windows 2000 au CERN : 12 - 14.11.01 (1/2 journée) Introduction to Windows 2000 at CERN :  14.11.01  (half-day) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Sécurité dans les installations cryogéniques : 21 - 22.11.2001 (2 demi-journées) Introduction to C Programming :  21- 23.11.01 (3 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) Contract Follow-up (F) : 26.11.01 (1/2 journée) Object-Oriented Analysis and Design :  27 - 30.11.2001  (4 days) Introduction to the CERN Engineering Data Management System :  30.11.2001 (1 day) Electromagnetic Compatibility (EMC): Introduction (bilingual) :  3.12.01 (half-day) Introduction to the CERN Engineering Data Management System : 07.12.2001...

  11. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Utilisation du simulateur Simplorer : 30.5 - 1.6.01 (3 jours) JAVA programming language level 1: 11-12.6.01 (2 days) LabView hands-on F ou E : 11.6.01 (1/2 journée) Comprehensive VHDL for EPLD/FPGA Design : 11 - 15.6.01 (5 days) Introduction au Langage C : 13 - 15.6.01 (3 jours) LabView Base 1 : 12 - 14.6.01 (3 jours) Habilitation électrique : superviseurs : 2 sessions d'une demi-journée les 12 et 19.6.01 Migration de LabVIEW 5 vers LabVIEW 6i Migration from LabVIEW 5 to LabVIEW 6I :  15.6.01 (1/2 journée/half-day) Introduction to Perl 5 : 2 - 3.7.01 (2 days) JAVA programming language level 2 : 4 - 6.7.01 (3 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from ...

  12. PLACES AVAILABLE

    CERN Multimedia

    Technical Traininf; Tel. 74924

    2001-01-01

    Places are available in the following courses: Electromagnetic Compatibility (EMC): Introduction (bilingual) :  3.12.01 (half-day) Habilitation électrique : superviseurs : 5.12.01 (1/2 journée) Introduction to the CERN Engineering Data Management System : 07.12.2001 (1 day) LabVIEW - Basics 1 :  10 - 12.12.01 (3 days) Introduction au PC et Windows 2000 : 12 & 14.12.01 (2 jours) LabVIEW - Basics 2 :  13 - 14.12.01 (2 days) Habilitation électrique : superviseurs : 17.12.2001 (1/2 journée) EXCEL 2000 - niveau 2 : 15 - 16.1.02 (2 jours) C++ Programming Level 2 - Traps and Pitfalls :  15 - 18.1.2002  (4 days) Nouveautés de WORD 2000 : 18.1.02 (1/2 journée) LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 - 25.1.02 (4 jours) Introduction au PC et à Windows 2000 au CERN : 29 - 30.1....

  13. Places available**

    CERN Document Server

    2003-01-01

    Places are available in the following courses : EXCEL 2000 - niveau 1 : 10 & 11.6.03 (2 jours) Conception de PCB rapides dans le flot Cadence : 11.6.03 (matin) EXCEL 2000 - level 1 : 12 & 13.6.03 (2 days) Introduction to PVSS : 16.6.03 (p.m.) Basic PVSS : 17 - 19.6.03 (3 days) Réalisation de PCB rapides dans le flot Cadence : 17.6.03 (matin) PVSS - JCOP Framework Tutorial : 20.6.03 (1 day) EXCEL 2000 - niveau 2 : 24 & 25.6.03 (2 jours) Siemens SIMATIC Training : Introduction to STEP7 : 3 & 4.6.03 (2 jours/2 days) STEP7 Programming : 16 - 20.6.03 (5 jours/5 days) Simatic Net Network : 26 & 27.6.03 (2 jours/2 days) These courses will be given in French or English following the requests. Programmation automate Schneider : Programmation automate Schneider TSX Premium - 1er niveau : 10 - 13.6.03 (4 jours) - audience : toute personne qui veux maitriser la msie en uvre et la programmation d'un automate TSX Premium - objectifs : maitriser la mise en uvre et la programmation d'un autom...

  14. Places available **

    CERN Document Server

    2003-01-01

    Places are available in the following courses: PIPES-2003 - Pratique du Sertissage de tubes métalliques et multicouches : 26.8.03 (stage pratique) The CERN Engineering Data Management System (EDMS) for Engineers : 27.8.03 (1 day, free of charge) CLEAN-2002 : Travailler en salle blanche : 4.9.03 (une demi-journée, séminaire gratuit) The CERN Engineering Data Management System (EDMS) for Local Administrators : 24 & 25.9.03 (2 days, free of charge) Siemens SIMATIC Training : Programmation STEP7 - niveau 1 : 29 - 2.10.03 (4 jours) - ouverture des inscriptions fin août Programmation STEP7 - niveau 2 : 13 - 17.10.03 (5 jours) - ouverture des inscriptions fin août Réseau Simatic Net : 22 & 23.10.03 (2 jours) - ouverture des inscriptions fin août CLEAN-2002 : Working in a Cleanroom : 23.20.03 (half day, free of charge) These courses will be given in French or Englis...

  15. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : FrontPage 2000 - niveau 1 : 20 & 21.5.03 (2 jours) PIPES-2003 : Pratique du sertissage de tubes métalliques et multicouches : 21.5.03 (1 jour) Introduction à la CAO Cadence : de la saisie de schéma Concept-HDL au PCB : 20 & 22.5.03 (2 jours) AutoCAD 2002 - niveau 2 : 3 & 4.6.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 5, 6, 12, 13, 26, 27.6.03 (6 jours) EXCEL 2000 - niveau 1 : 10 & 11.6.03 (2 jours) Conception de PCB rapides dans le flot Cadence : 11.6.03 (matin) EXCEL 2000 - level 1 : 12 & 13.6.03 (2 days) PowerPoint 2000 (F) : 17 & 18.6.03 (2 jours) Réalisation de PCB rapides dans le flot Cadence : 17.6.03 (matin) FrontPage 2000 - niveau 2 : 19 & 20.6.03 (2 jours) LabView DSC (langue à décider/language to be defined) : 19 & 20.6.03 EXCEL 2000 - niveau 2 : 24 & 25.6.03 (2 jours) Siemens SIMATIC Training: Introduction to STEP7 : 3 & 4.6.03 (2 days) STEP7 Programming : 16 - 20.6.03 (5 days) Simatic...

  16. Places available**

    CERN Document Server

    2003-01-01

    Places are available in the following courses : FrontPage 2000 - niveau 1: 20 & 21.5.03 (2 jours) PIPES-2003 : Pratique du sertissage de tubes métalliques et multicouches: 21.5.03 (1 jour) Introduction à la CAO Cadence: de la saisie de schéma Concept-HDL au PCB : 20 & 22.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (E): 5, 6, 12, 13, 26, 27.6.03 (6 days) EXCEL 2000 - niveau 1: 10 & 11.6.03 (2 jours) Conception de PCB rapides dans le flot Cadence: 11.6.03 (matin) EXCEL 2000 - level 1: 12 & 13.6.03 (2 days) Introduction to PVSS: 16.6.03 (half-day, pm) Basic PVSS: 17 - 19.6.03 (3 days) Réalisation de PCB rapides dans le flot Cadence: 17.6.03 (matin) LabView DSC (language to be defined): 19 & 20.6.03 PVSS - JCOP Framework Tutorial: 20.6.03 (1 day) EXCEL 2000 - niveau 2: 24 & 25.6.03 (2 jours) Siemens SIMATIC Training: Introduction to STEP7: 3 & 4.6.03 (2 days) STEP7 Programming: 16 - 20.6.03 (5 days) Simatic Net Network: 26 & 27.6.03 (2 days) These courses will be given...

  17. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: November 2002   Java Programming Language level 1 :  28 & 29.11.02  (2 days) December 2002   LabVIEW - DSC (English) :  2 - 3.12.02  (2 days) FileMaker (Français) :  2 - 5.12.02  (4 jours) PCAD Schémas - Débutants :  5 & 6.12.02  (2 jours) PCAD PCB - Débutants :  9 - 11.12.02  (3 jours) FrontPage 2000 - level 1:  9 & 10.12.02  (2 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training M...

  18. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: LabView Hands-on (bilingue/bilingual - gratuit/free of charge) : 13.9.02 (a.m.) LabView DAQ Hands-on (bilingue/bilingual - gratuit/free of charge) : 13.9.02 (p.m.) AutoCAD 2002 - niveau 1 : 19, 20, 26, 27.9.02 (4 jours) LabView Base 1 : 23 - 25.9.02 (3 jours) LabView DAQ (E) : 26 - 27.9.02 (2 days) AutoCAD Mechanical 6 PowerPack (F) : 30.9, 1, 2, 9, 10, 11.10.02 (6 jours) CLEAN-2002 : Working in a Cleanroom (free of charge) : 10.10.02 (half-day, p.m.) AutoCAD 2002 - niveau 2 : 14 - 15.10.02 (2 jours) AutoCAD 2002 - Level 1 : 17, 18, 24, 25.10.02 (4 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Of...

  19. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Introduction to Oracle 8i : SQL and PL/SQL:  7 - 11.10.02  (5 days) CLEAN-2002 : Working in a Cleanroom (free of charge):  10.10.02  (half-day, p.m.) AutoCAD 2002 - niveau 2 :  14 - 15.10.02  (2 jours) Introduction à DesignSpace :  16.10.02  (1 journée) Introduction to DesignSpace:  17.10.02  (1 day) AutoCAD 2002 - Level 1:  17, 18, 24, 25.10.02  (4 days) AutoCAD Mechanical 6 PowerPack (F) :  21, 22, 23.10 et 4, 5, 6.11.02  (6 jours) Introduction à ANSYS/Introduction to ANSYS (langue à définir suivant demande/ Language to be chosen according to demand):  21 - 25.10.02  (5 jours/days) HREF-2002: Helium Refrigeration Techniques (English-French, bilingual) :  21 - 25.10.2002  (7 half days) HREF-2002: Techniques de la Réfri...

  20. Places available**

    CERN Document Server

    2003-01-01

    Places are available in the following courses : The CERN EDMS for Local Administrators : 24 & 25.9.03 (2 days, free of charge) HeREF-2003 : Techniques de la réfrigération Hélium (cours en français avec support en anglais) : 6 - 10.10.2003 (7 demi-journées) The Java Programming Language Level 1 : 6 - 7.10.2003 (2 days) Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) AutoCAD 2002 - niveau 1 : 20, 21, 27, 28.10.03 (4 jours) CLEAN-2002 : Working in a Cleanroom : 23.10.03 (half day, free of charge) AutoCAD 2002 - Level 1 : 3, 4, 12, 13.11.03 (4 days) AutoCAD 2002 - niveau 2 : 10 & 11.11.03 (2 jours) ACCESS 2000 - niveau 1 : 13 & 14.11.03 (2 jours) AutoCAD Mechanical 6 PowerPack (E) : 17, 18, 24, 25.11 & 1, 2.12.03 (6...

  1. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Introduction à DesignSpace :  16.10.02  (1 journée) AutoCAD Mechanical 6 PowerPack (F) :  21, 22, 23.10 et 4, 5, 6.11.02  (6 jours) Introduction à ANSYS 21 - 25.10.02  (5 jours/days) HREF-2002: Helium Refrigeration Techniques (English-French, bilingual) :  21 - 25.10.2002  (7 half days) LabVIEW Basics 1 (English):  21 - 23.10.02  (3 days) LabVIEW Basics 2 (English):  24 & 25.10.02  (2 days) Oracle 8i : Access the Database with Java:  7 & 8.11.02  (2 days) AutoCAD 2002 - niveau 2 :  7 & 8.11.02  (2 jours) AutoCAD 2002 - Level 1:  14, 15, 21, 22.11.02  (4 days) LabVIEW - Advanced (English) :  18 - 20.11.2002  (3 days) AutoCAD 2002 - niveau 1 :  19, 20, 25, 26.11.02 (4 jours) Oracle iDS Designer: First Class:&...

  2. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Java 2 Enterprise Edition - Part 2: Enterprise JavaBeans:  18 - 20.9.02  (3 days) AutoCAD 2002 - niveau 1 :  19, 20, 26, 27.9.02  (4 jours) LabView Base 1 :  23 - 25.9.02  (3 jours) Object-Oriented Analysis & Design using UML:  25 - 27.9.02  (3 days) LabView DAQ (E):  26 - 27.9.02  (2 days) Introduction to Oracle 8i : SQL and PL/SQL:  7 - 11.10.02  (5 days) CLEAN-2002 : Working in a Cleanroom (free of charge):  10.10.02  (half-day, p.m.) AutoCAD 2002 - niveau 2 :  14 - 15.10.02  (2 jours) Introduction à DesignSpace :  16.10.02  (1 journée) Introduction to DesignSpace:  17.10.02  (1 day) AutoCAD 2002 - Level 1:  17, 18, 24, 25.10.02  (4 days) AutoCAD Mechanical 6 PowerPack (F) :  21, 22, 23.10 et 4, 5, 6.11....

  3. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: November 2002   LabView hands-on (bilingue/bilingual): 5.11.02 (matin/morning) LabView DAQ hands-on (bilingue/bilingual):  5.11.02  (après-midi afternoon) PCAD Schémas - Débutants :  5 & 6.11.02  (2 jours) PCAD PCB - Débutants :  9 - 11.11.02  (3 jours) Introduction au PC et Windows 2000 au CERN :  6 & 7.11.02  (2 jours) Oracle 8i : Access the Database with Java :  7 & 8.11.02  (2 days) Introduction to PVSS (free of charge):  11.11.2002 pm  (1/2 day) Basic PVSS:  12 - 14.11.02  (3 days) EXCEL 2000 - niveau 1 :  12 & 13.11.02  (2 jours) CLEAN-2002: Working in a Cleanroom (English, free of charge):  13.11.2002  (afternoon) LabView Base 1 :  13 - 15.11.02  (3 jours) AutoCAD 2002 - niveau 1 :  14, 15, 21, 22.11.02  (4 jours) LabVIEW - Advanced:  18 - 20.11.02  (3 days) Hands-on Object-Oriented Design and Programming with C++ :  19 - 21.11.02  (3 days)  LabVIEW - Basics 2:  21 - 22.11.02 ...

  4. Places available**

    CERN Document Server

    2003-01-01

    Places are available in the following courses: CLEAN-2002 : Travailler en salle blanche (séminaire gratuit) : 4.9.03 (une demi-journée) The CERN EDMS for Local Administrators (free of charge) : 24 & 25.9.03 (2 days) HeREF-2003 : Techniques de la réfrigération Hélium (cours en français avec support en anglais) : 6 - 10.10.2003 (7 demi-journées) The Java Programming Language Level 1 : 6 - 7.10.2003 (2 days) Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) AutoCAD 2002 - niveau 1 : 20, 21, 27, 28.10.03 (4 jours) CLEAN-2002 : Working in a Cleanroom (free of charge) : 23.10.03 (half day) AutoCAD Mechanical 6 PowerPack (E) : 23, 24, 30, 31.10 & 12, 13.11.03 (6 days) AutoCAD 2002 - niveau 2 : 10 & 11.11.03 (2 jours)...

  5. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: The CERN EDMS for Local Administrators : 24 & 25.9.03 (2 days, free of charge) HeREF-2003 : Techniques de la réfrigération Hélium cours en français avec support en anglais) : 6 - 10.10.2003 (7 demi-journées) The Java Programming Language Level 1 : 6 - 7.10.2003 (2 days) Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) AutoCAD 2002 - niveau 1 : 20, 21, 27, 28.10.03 (4 jours) CLEAN-2002 : Working in a Cleanroom : 23.10.03 (half day, free of charge) AutoCAD 2002 - Level 1 : 3, 4, 12, 13.11.03 (4 days) AutoCAD 2002 - niveau 2 : 10 & 11.11.03 (2 jours) ACCESS 2000 - niveau 1 : 13 & 14.11.03 (2 jours) AutoCAD Mechanical 6 PowerPack (E) : 17, 18, 24, 25.11 & 1, 2.12.03 (6 days) FrontPage 2000 - niveau 1 : 20 & 21.11.03 (2 jours) MAGNE-03 : Magnétisme pour l'électrotechnique : 25 - 27.11.03 (3 jours) ...

  6. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: LabView hands-on (bilingue/bilingual): 5.11.02 (matin/morning) LabView DAQ hands-on (bilingue/bilingual):  5.11.02  (après-midi afternoon) Introduction au PC et Windows 2000 au CERN:  6 & 7.11.02  (2 jours) Oracle 8i : Access the Database with Java:  7 & 8.11.02  (2 days) AutoCAD 2002 - niveau 2:  7 & 8.11.02  (2 jours) Introduction to PVSS (free of charge):  11.11.2002 pm  (1/2 day) Basic PVSS:  12 - 14.11.02  (3 days) EXCEL 2000 - niveau 1:  12 & 13.11.02  (2 jours) CLEAN-2002: Working in a Cleanroom (English, free of charge):  13.11.2002  (afternoon) LabView Base 1 :  13 - 15.11.02  (3 jours) AutoCAD 2002 - Level 1:  14, 15, 21, 22.11.2002  (4 days) LabVIEW - Advanced:  18 - 20.11.02  (3 days) Auto...

  7. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: PIPES-2003 - Pratique du sertissage de tubes métalliques et multicouches :26.8.03(stage pratique) The CERN EDMS for Engineers (free of charge) : 27.8.03 (1 day) CLEAN-2002 : Travailler en salle blanche (séminaire gratuit) : 4.9.03(une demi-journée) The CERN EDMS for Local Administrators (free of charge) : 24 & 25.9.03 (2 days) HeREF-2003 : Techniques de la réfrigération Hélium (cours en français avec support en anglais) : 6 - 10.10.2003 (7 demi-journées) The Java Programming Language Level 1 : 6 - 7.10.2003 (2 days) Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) AutoCAD 2002 - niveau 1 : 20, 21, 27, 28.10.03 (4 jours) CLEAN-2002 : Working in a Cleanroom (free of charge) : 23.10.03 (half day) AutoCAD Mechanical 6 PowerPack (E) : 23, 24, 30, 31.10 & 12, 13.11.03 (6 days) AutoCAD 2002 - niveau 2...

  8. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: The CERN EDMS for Local Administrators (free of charge) : 24 & 25.9.03 (2 days) HeREF-2003 : Techniques de la réfrigération Hélium (cours en français avec support en anglais) : 6 - 10.10.2003 (7 demi-journées) The Java Programming Language Level 1 : 6 - 7.10.2003 (2 days) Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) AutoCAD 2002 - niveau 1 : 20, 21, 27, 28.10.03 (4 jours) CLEAN-2002 : Working in a Cleanroom (free of charge) : 23.10.03 (half day) AutoCAD Mechanical 6 PowerPack (E) : 23, 24, 30, 31.10 & 12, 13.11.03 (6 days) AutoCAD 2002 - niveau 2 : 10 & 11.11.03 (2 jours) ACCESS 2000 - niveau 1 : 13 & 14.11.03 (2 jours) FrontPage 2000 - niveau 1 : 20...

  9. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: October 2002   Introduction to the CERN Engineering Data Management System (free of charge):  29.10.2002  (1 day) The CERN EDMS for Advanced users (free of charge):  30.10.2002  (1 day) November 2002   LabView hands-on (bilingue/bilingual): 5.11.02 (matin/morning) LabView DAQ hands-on (bilingue/bilingual):  5.11.02  (après-midi afternoon) Introduction au PC et Windows 2000 au CERN :  6 & 7.11.02  (2 jours) Oracle 8i : Access the Database with Java:  7 & 8.11.02  (2 days) AutoCAD 2002 - niveau 2 :  7 & 8.11.02  (2 jours) Introduction to PVSS (free of charge):  11.11.2002 pm  (1/2 day) Basic PVSS:  12 - 14.11.02  (3 days) EXCEL 2000 - niveau 1 :  12 & 13.11.02  (2 jours) CLEAN-2002: Working in a Cleanroom (English, free ...

  10. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Introduction to Oracle 8i : SQL and PL/SQL:  7 - 11.10.02  (5 days) CLEAN-2002 : Working in a Cleanroom (free of charge):  10.10.02  (half-day, p.m.) LabView Hands-on (bilingue/bilingual) : 10.10.02 (matin/morning) LabView DAQ Hands-on (bilingue/bilingual)  10.10.02 (après-midi /afternoon) Introduction à DesignSpace :  16.10.02  (1 journée) Introduction to DesignSpace:  17.10.02  (1 day) AutoCAD Mechanical 6 PowerPack (F) :  21, 22, 23.10 et 4, 5, 6.11.02  (6 jours) Introduction à ANSYS/Introduction to ANSYS (langue à définir suivant demande/ Language to be chosen according to demand):  21 - 25.10.02  (5 jours/days) HREF-2002: Helium Refrigeration Techniques (English-French, bilingual) :  21 - 25.10.2002  (7 half days) HREF-2002: Techniques de la...

  11. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: November 2002   Hands-on Object-Oriented Design and Programming with C++:  19 - 21.11.02  (3 days)  December 2002   LabVIEW - DSC (English) :  2 - 3.12.02  (2 days) AutoCAD 2002 - niveau 2 :  2 & 3.12.02  (2 jours) FileMaker (Français) :  2 - 5.12.02  (4 jours) PCAD Schémas - Débutants :  5 & 6.12.02  (2 jours) PCAD PCB - Débutants :  9 - 11.12.02  (3 jours) FrontPage 2000 - level 1:  9 & 10.12.02  (2 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisiona...

  12. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: November 2002   Introduction to PVSS (free of charge): 11.11.02  (afternoon) EXCEL 2000 - niveau 1 :  12 & 13.11.02  (2 jours) CLEAN-2002: Working in a Cleanroom (English, free of charge):  13.11.2002  (afternoon) AutoCAD 2002 - niveau 1 :  14, 15, 21, 22.11.02  (4 jours) Hands-on Object-Oriented Design and Programming with C++:  19 - 21.11.02  (3 days)  EXCEL 2000 - niveau 2 :  25 & 26.11.02  (2 jours) FrontPage 2000 - niveau 1 :  27 & 28.11.02  (2 jours) December 2002   LabVIEW - DSC (English) :  2 - 3.12.02  (2 days) AutoCAD 2002 - niveau 2 :  2 & 3.12.02  (2 jours) FileMaker (Français) :  2 - 5.12.02  (4 jours) PCAD Schémas - Débutants :  5 & 6.12.02 ...

  13. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: LabView Base 1 :  23 - 25.9.02  (3 jours) Object-Oriented Analysis & Design using UML:  25 - 27.9.02  (3 days) LabView DAQ (E):  26 - 27.9.02  (2 days) Introduction to Oracle 8i : SQL and PL/SQL:  7 - 11.10.02  (5 days) CLEAN-2002 : Working in a Cleanroom (free of charge):  10.10.02  (half-day, p.m.) AutoCAD 2002 - niveau 2 :  14 - 15.10.02  (2 jours) Introduction à DesignSpace :  16.10.02  (1 journée) Introduction to DesignSpace:  17.10.02  (1 day) AutoCAD 2002 - Level 1:  17, 18, 24, 25.10.02  (4 days) AutoCAD Mechanical 6 PowerPack (F) :  21, 22, 23.10 et 4, 5, 6.11.02  (6 jours) Introduction à ANSYS/Introduction to ANSYS (langue à définir suivant demande/ Language to be chosen according to demand):...

  14. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74460

    2001-01-01

    Places are available in the following courses: Introduction à PowerPoint : 26.2.01 (1 journée) Programmation TSX Premium 1 : 26.2 - 2.3.01 (5 jours) Premiers pas avec votre PC : 27.2 - 2.3.01 (4 matins) C++ for Particle Physicists :  5 - 9.3.01 (6*3 hour lectures) The CERN Engineering Data Management System for Electronic Design : 6.3.01 (1 day) The CERN Engineering Data Management System for Electronic Design : 7.3.01 (1 day) EXCEL : 6, 7 et 13, 14.3.01 (4 jours) The JAVA programming language level 2 : 12 - 14.3.01 (3 days) Nouveautés de FileMaker : 20 - 23.03.01 (4 matins) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be acc...

  15. PLACES AVAILABLE

    CERN Multimedia

    Enseignement Technique; Tél. 74924; Technical Training; Monique Duval; Tel. 74924

    2000-01-01

    Places are available in the following courses : Premiers pas avec votre PC 12 - 15.9.00 (4 demi-journées) WORD 20, 21 et 26, 27.9.2000 (4 jours) JAVA programming level 1 25 - 26.9.2000 (2 days) Gaz inflammables 1 26.9.2000 (1 journée) Advanced aspects of PERL 5 6.10.2000 (1 day) Initiation au WWW 10 - 12.10.00 (3 demi-journées) WORD : importer et manipuler des images 16.10.2000 (1 journée) FileMaker 17, 18 et 24, 25.10.00 (4 jours) Nouveautés de WORD 19 et 20.10.2000 (2 jours) ACCESS 1er niveau 30 - 31.10.00 (2 jours)Introduction à PowerPoint 6.11.00 (1 journée)Nouveautés d’EXCEL 7.11.2000(4 demi-journées)Excel 13, 14 et 20, 21.11.00 (4 jours) LabView hands-on 13.11.2000(4 hours)LabView Basics 1 14 - 16.11.2000 (3 days) MS-Project 1er niveau 14-17.11.00 (4 demi-journées) If you wish to participate in one of these courses, please discuss with your supervisor and apply elec...

  16. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: MS-Project 2000 - niveau 1 : 15 - 18.10.01 (4 demi-journées) LabView Base 2 : 18 & 19.10.01 (2 jours) WORD 2000 : importer et manipuler des images : 19.10.01 (1 journée) Contract Follow-up (F) : 30.10.01 (1/2 journée) The CERN Engineering Data Management System for Electronics Design :  30.10.01 (1 day) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) The Java programming language Level 1: 8 - 9.11.01 (2 days) LabView Base 1 : 12 - 14.11.01 (3 jours) Automates et réseaux de terrain : 13 & 14.11.01 (2 jours) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to XML :  19 - 20.11.01 (2 days) Programming TSX Premium 1 :  19 - 23.11.01  (5 days) Introduction to C Programming :  21- 23.11.01 (3 days) The Java programming language Level 2:  26 - 28.11.01 (3 days) Programmation TSX Premium 2 : 26 ...

  17. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Introduction à Windows 2000 au CERN : 2 sessions de _ journée les 24 et 25.9.01 PROFIBUS : 25 - 26.9.01 (2 jours) PROFIBUS : 27 - 28.9.01 (2 days) PowerPoint 2000 : 1 et 2.10.01 (2 jours) EXCEL 2000 - niveau 1 : 3 et 4.10.01 (2 jours) Automates et réseaux de terrain : 3 - 4.10.2001 (2 jours) PCAD Schémas - débutants : 4 - 5.10.01 (2 jours) Introduction à Outlook : 5.10.01 (1 journée) Frontpage 2000 - niveau 1 : 8 et 9.10.01 (2 jours) PCAD PCB - débutants : 8 - 10.10.01 (3 jours) C++ for Particle Physicists : 8 - 12.10.01 (6 3-hour lectures) MS-Project 2000 - niveau 1 : 15 - 18.10.01 (4 demi-journées) LabView Basics 1 :  15 - 17.10.01  (3 days) Programmation TSX Premium 1 : 15 - 19.10.01 (5 jours) WORD 2000 : importer et manipuler des images : 19.10.01 (1 journée) Programmation TSX Premium 1 : 22 - 26.10.01...

  18. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: LabVIEW - Basics 1 :  10 - 12.12.01 (3 days) Introduction to XML :  12 & 13.12.01 (2 days) Introduction au PC et Windows 2000 : 12 & 14.12.01 (2 jours) LabVIEW - Basics 2 :  13 - 14.12.01 (2 days) Habilitation électrique : superviseurs : 17.12.2001 (1/2 journée) MS-Project 2000 : 10 & 11.01.02 (2 jours) EXCEL 2000 - niveau 2 : 15 - 16.1.02 (2 jours) Sécurité dans les installations cryogéniques: 15-17.1.2002 (2 demi-journées) C++ Programming Level 2 - Traps and Pitfalls :  15 - 18.1.2002  (4 days) ELEC-2002 Winter Term: Readout and system electronics for Physics  15.1.2002 - 7.2.2002 (8 half- days) Nouveautés de WORD 2000 : 18.1.02 (1/2 journée) LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 -...

  19. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Introduction à Windows 2000 au CERN : 2 sessions de _ journée les 24 et 25.9.01 PROFIBUS : 25 - 26.9.01 (2 jours) PROFIBUS : 27 - 28.9.01 (2 days) EXCEL 2000 - niveau 1 : 3 et 4.10.01 (2 jours) Automates et réseaux de terrain : 3 - 4.10.2001 (2 jours) Introduction à Outlook : 5.10.01 (1 journée) Frontpage 2000 - niveau 1 : 8 et 9.10.01 (2 jours) C++ for Particle Physicists : 8 - 12.10.01 (6 lectures) MS-Project 2000 - niveau 1 : 15 - 18.10.01 (4 demi-journées) Programmation TSX Premium 1 : 15 - 19.10.01 (5 jours) WORD 2000 : importer et manipuler des images : 19.10.01 (1 journée) Programmation TSX Premium 1 : 22 - 26.10.01 (5 jours) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) The Java programming language Level 1: 8 - 9.11.01 (2 days) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to XML :  19 - 20.11.01 (2...

  20. PLACES AVAILABLE

    CERN Multimedia

    TECHNICAL TRAINING; Tel. 74460

    2001-01-01

    Places are available in the following courses: MS-Project 1er niveau : 20 - 23.2.01 (4 matins) Architecture d'automatisme : 20 - 21.2.01 (2 jours) Introduction à PowerPoint : 26.2.01 (1 journée) Programmation TSX Premium 1 (Schneider) : 26.2 - 2.3.01 (5 jours) Premiers pas avec votre PC : 27.2 - 2.3.01 (4 matins) C++ for Particle Physicists : 5 - 9.3.01 (6*3 hour lectures) EXCEL : 6, 7 et 13, 14.3.01 (4 jours) The JAVA programming language level 2 :  12 - 14.3.01 (3 days) Nouveautés de FileMaker :  20 - 23.03.01 (4 matins) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

  1. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel 74924

    2002-01-01

    Places are available in the following courses: LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 - 25.1.02 (4 jours) MS-Project 2000 : 24 & 25.01.02 (2 jours) Introduction au PC et à Windows 2000 au CERN : 29 - 30.1.02 (2 jours) LabView Base 1 : 4 - 6.2.02 (3 jours) LabView DAQ (E) : 7 & 8.02.02 (2 days) Hands-on Object-Oriented Design & Programming with Java : 11 - 13.02.02 (3 days) C++ for Particle Physicists : 11 - 15.3.2002 (6 * 3 hour lectures) Cours sur la migration AutoCAD : AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé sur AutoCAD 2002 (5 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO ...

  2. PLACES AVAILABLE

    CERN Multimedia

    Enseignement Technique; Tel. 74924

    2001-01-01

    Places are available in the following courses: MS-Project 2000 : 10 & 11.01.02 (2 jours) EXCEL 2000 - niveau 2 : 15 - 16.1.02 (2 jours) Sécurité dans les installations cryogéniques: 15-17.1.2002 (2 demi-journées) C++ Programming Level 2 - Traps and Pitfalls :  15 - 18.1.2002  (4 days) ELEC-2002 Winter Term: Readout and system electronics for Physics  15.1.2002 - 7.2.2002 (8 half- days) Nouveautés de WORD 2000 : 18.1.02 (1/2 journée) LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 - 25.1.02 (4 jours) MS-Project 2000 : 24 & 25.01.02 (2 jours) Introduction au PC et à Windows 2000 au CERN : 29 - 30.1.02 (2 jours) LabView Base 1 : 4 - 6.2.02 (3 jours) LabView DAQ  (E) :  7 & 8.02.02 (2 days) Hands-on Object-Oriented Design & Programming with Java :&nbs...

  3. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: EXCEL 2000 - niveau 1 : 3 et 4.10.01 (2 jours) Automates et réseaux de terrain : 3 - 4.10.2001 (2 jours) Introduction à Outlook : 5.10.01 (1 journée) C++ for Particle Physicists : 8 - 12.10.01 (6 lectures) Cadence Board Design tools : Upgrading to release 14 : 3 1-day sessions on 9, 10 & 11.10.01 MS-Project 2000 - niveau 1 : 15 - 18.10.01 (4 demi-journées) LabView Base 2 : 18 & 19.10.01 (2 jours) WORD 2000 : importer et manipuler des images : 19.10.01 (1 journée) The CERN Engineering Data Management System for Electronics Design :  30.10.01 (1 day) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) The Java programming language Level 1: 8 - 9.11.01 (2 days) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to XML :  19 - 20.11.01 (2 days) Programming TSX Premium 1 :  19 - 23.11.01  (5 days) Introd...

  4. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2002-01-01

    Places are available in the following courses: LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 - 25.1.02 (4 jours) MS-Project 2000 : 22, 24 & 25.01.02 (3 jours) Introduction au PC et à Windows 2000 au CERN : 29 - 30.1.02 (2 jours) LabView Base 1 : 4 - 6.2.02 (3 jours) LabView DAQ  (E) :  7 & 8.02.02 (2 days) Hands-on Object-Oriented Design & Programming with Java :  11 - 13.02.02 (3 days) PVSS basics :  11 - 15.2.02 (5 days) Introduction à Windows 2000 : 18.2.02 (1 demi-journée) Introduction to the CERN Engineering Data Management System :  20.2.02 (1 day) The CERN Engineering Data Management System for Advanced users :  21.2.02  (1 day) C++ for Particle Physicists :  11 - 15.3.2002  (6 * 3 hour lectures) Cours sur la migration AutoCAD : AutoCAD : Mise à...

  5. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Cadence Board Design tools : Upgrading to release 14 :  3 1-day sessions on 9, 10 & 11.10.01 MS-Project 2000 - niveau 1 : 15 - 18.10.01 (4 demi-journées) LabView Base 2 : 18 & 19.10.01 (2 jours) WORD 2000 : importer et manipuler des images : 19.10.01 (1 journée) Contract Follow-up (F) :  30.10.01 (1/2 journée) The CERN Engineering Data Management System for Electronics Design :  30.10.01 (1 day) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) The Java programming language Level 1: 8 - 9.11.01 (2 days) LabView Base 1 : 12 - 14.11.01 (3 jours) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to XML :  19 - 20.11.01 (2 days) Programming TSX Premium 1 :  19 - 23.11.01  (5 days) Introduction to C Programming :  21- 23.11.01 (3 days) The Java programming language Level 2:  26 - 28.11.01 (...

  6. Places disponibles/Places available

    CERN Multimedia

    2004-01-01

    Si vous désirez participer à l'un des cours suivants, veuillez en discuter avec votre superviseur et vous inscrire électroniquement en direct depuis les pages de description des cours dans le Web que vous trouvez à l'adresse : http://www.cern.ch/Training/ ou remplissez une « demande de formation » disponible auprès du Secrétariat de votre Division ou de votre DTO (Délégué divisionnaire à la formation). Les places seront attribuées dans l'ordre de réception des inscriptions. If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Off...

  7. Influence of glutamate on growth, sporulation, and spore properties of Bacillus cereus ATCC 14579 in defined medium

    NARCIS (Netherlands)

    Vries, de Y.P.; Atmadja, R.D.; Hornstra, L.M.; Vos, de W.M.; Abee, T.

    2005-01-01

    A chemically defined medium in combination with an airlift fermentor system was used to study the growth and sporulation of Bacillus cereus ATCC 14579. The medium contained six amino acids and lactate as the main carbon sources. The amino acids were depleted during exponential growth, while lactate

  8. Sporulation environment of emetic toxin-producing Bacillus cereus strains determines spore size, heat resistance and germination capacity

    NARCIS (Netherlands)

    Voort, van der M.; Abee, T.

    2013-01-01

    Aim Heat resistance, germination and outgrowth capacity of Bacillus cereus spores in processed foods are major factors in causing the emetic type of gastrointestinal disease. In this study, we aim to identify the impact of different sporulation conditions on spore properties of emetic

  9. Effect of environmental conditions and lesion age on sporulation of Phytophthora ramorum on California bay laurel, rhododendron, and camellia

    Science.gov (United States)

    Steve Tjosvold; David Chambers; Sylvia Mori

    2013-01-01

    The objective of our research was to determine the environmental conditions and lesion age favorable for Phytophthora ramorum sporulation under field conditions. For 2 years, new camellia, rhododendron, and California bay laurel (Umbellaria californica (Hook. & Arn.) Nutt.) nursery stock were seasonally inoculated (every 3 months) on foliage....

  10. Head of household education level as a factor influencing whether delivery takes place in the presence of a skilled birth attendant in Busia, Uganda: a cross-sectional household study.

    Science.gov (United States)

    Vallières, Frédérique; Hansen, Alexandria; McAuliffe, Eilish; Cassidy, Emma Louise; Owora, Paul; Kappler, Sam; Gathuru, Evelyn

    2013-02-21

    Assistance during delivery by a skilled attendant is recommended as a means to reduce child and maternal mortality. Globally, higher levels of maternal education have been associated with better health behaviours at delivery. However, given that heads of households tend to be the decision makers regarding accessing healthcare, some educated mothers may find themselves prevented from accessing healthcare at the point of delivery. We examined the association between head of household education level and health seeking behaviours at delivery across a sample of 392 households. Chi-squared analysis and odds ratios were calculated to measure the strength of the relationship between no, some primary, or some secondary or higher education attained by the head of household and the presence or absence of a skilled birth attendant at that child's birth, and whether the birth took place at a health facility. Heads of household (n = 392) were predominantly male (93.4% [(90.9%, 95.8%), a = 0.05]). We found a significant difference in skilled birth attendance between heads of households with some primary education and heads of household with some secondary education or higher (χ2 (1) = 6.231, p birth attendant (OR = 1.5,[1.1,2.1]). The difference in health centre delivery between heads of household with a primary education and heads of household with a secondary or higher education was also significant (χ2 (1) = 7.519, p rates of health centre deliveries and utilization of services provided by skilled health workers might lie, in part, in increasing overall education levels of heads of households, specifically the education of male heads of households.

  11. Taking Leave?

    CERN Multimedia

    2000-01-01

    Planning a holiday? Then if you're a member of the personnel, you'll need to use the Laboratory's new leave system that will be put in place on 1 October. Leave allocations don't change - you are entitled to just as much holiday as before - but instead of being credited annually, your leave will be credited on a monthly basis, and this information will be communicated on your salary slip. The reason for the change is that with the various new leave schemes such as Recruitment by Saved Leave (RSL) and the Progressive Retirement Programme (PRP), a streamlined procedure was required for dealing with all kinds of leave. In the new system, each member of the personnel will have leave accounts to which leave will be credited monthly from the payroll and debited each time an absence is registered in the CERN Electronic Document Handling system (EDH). Leave balances will appear on monthly pay slips, and full details of leave transactions and balances will be available through EDH at all times. As the leave will be c...

  12. Take heart!

    CERN Multimedia

    Alizée Dauvergne

    2010-01-01

    Recently, ten new semi-automatic defibrillators were installed at various locations around CERN. This is a preventive measure intended to provide cardiac arrest victims with the best possible response. The first responder could be you!   The Director-General has welcomed the initiative of the Medical Service and Fire Brigade for the installation of ten new semi-automatic defibrillators. You have probably seen them on your way to the restaurant, for example:  brand new semi-automatic defibrillators, ready for an emergency. Housed in a white wall-mounted case, the bright red defibrillators are marked with a white heart symbol crossed by a lightning bolt (see photo). The defibrillator is designed so that anyone can use it. “Anyone can use it, you don’t need to be a health professional,” says Dr Reymond from CERN's Medical Service. Together with the CERN Fire Brigade, he is behind the initiative to have these units put in place. And with good reason, as the unit...

  13. Assessment of pheromone production and response in fission yeast by a halo test of induced sporulation

    DEFF Research Database (Denmark)

    Egel, R; Willer, M; Kjaerulff, S

    1994-01-01

    of exogenous pheromone. The tester strains are heterozygous for mating type, are non-switching, and are mutated in one of the early subfunctions (either mat1-Mc or mat1-Pc), so that meiosis is only induced after exposure to exogenous pheromone (M-factor or P-factor, respectively). Pheromone activity...... is assessed as an iodine-positive halo of sporulation surrounding the pheromone source, and the width of the halo is related to the amount of pheromone being produced. The assay is sufficiently sensitive to monitor the low amount of M-factor produced by an M mam1 strain, and its sensitivity towards P......-factor is greatly increased by using a hyper-sensitive tester strain lacking the Sxa2 protease that is believed to degrade this pheromone. We also demonstrate that the production of P-factor is very much stimulated by exposure of P cells to M-factor....

  14. A model of cell-wall dynamics during sporulation in Bacillus subtilis

    Science.gov (United States)

    Yap, Li-Wei; Endres, Robert G.

    To survive starvation, Bacillus subtilis forms durable spores. After asymmetric cell division, the septum grows around the forespore in a process called engulfment, but the mechanism of force generation is unknown. Here, we derived a novel biophysical model for the dynamics of cell-wall remodeling during engulfment based on a balancing of dissipative, active, and mechanical forces. By plotting phase diagrams, we predict that sporulation is promoted by a line tension from the attachment of the septum to the outer cell wall, as well as by an imbalance in turgor pressures in the mother-cell and forespore compartments. We also predict that significant mother-cell growth hinders engulfment. Hence, relatively simple physical principles may guide this complex biological process.

  15. What is taking place in science classrooms?: A case study analysis of teaching and learning in seventh-grade science of one Alabama school and its impact on African American student learning

    Science.gov (United States)

    Norman, Lashaunda Renea

    This qualitative case study investigated the teaching strategies that improve science learning of African American students. This research study further sought the extent the identified teaching strategies that are used to improve African American science learning reflect culturally responsive teaching. Best teaching strategies and culturally responsive teaching have been researched, but there has been minimal research on the impact that both have on science learning, with an emphasis on the African American population. Consequently, the Black-White achievement gap in science persists. The findings revealed the following teaching strategies have a positive impact on African American science learning: (a) lecture-discussion, (b) notetaking, (c) reading strategies, (d) graphic organizers, (e) hands-on activities, (f) laboratory experiences, and (g) cooperative learning. Culturally responsive teaching strategies were evident in the seventh-grade science classrooms observed. Seven themes emerged from this research data: (1) The participating teachers based their research-based teaching strategies used in the classroom on all of the students' learning styles, abilities, attitudes towards science, and motivational levels about learning science, with no emphasis on the African American student population; (2) The participating teachers taught the state content standards simultaneously using the same instructional model daily, incorporating other content areas when possible; (3) The participating African American students believed their seventh-grade science teachers used a variety of teaching strategies to ensure science learning took place, that science learning was fun, and that science learning was engaging; (4) The participating African American students genuinely liked their teacher; (5) The participating African American students revealed high self-efficacy; (6) The African American student participants' parents value education and moved to Success Middle School

  16. Effects of selected herbicides and fungicides on growth, sporulation and conidial germination of entomopathogenic fungus Beauveria bassiana.

    Science.gov (United States)

    Celar, Franci A; Kos, Katarina

    2016-11-01

    The in vitro fungicidal effects of six commonly used fungicides, namely fluazinam, propineb, copper(II) hydroxide, metiram, chlorothalonil and mancozeb, and herbicides, namely isoxaflutole, fluazifop-P-butyl, flurochloridone, foramsulfuron, pendimethalin and prosulfocarb, on mycelial growth, sporulation and conidial germination of entomopathogenic fungus Beauveria bassiana (ATCC 74040) were investigated. Mycelial growth rates and sporulation at 15 and 25 °C were evaluated on PDA plates containing 100, 75, 50, 25, 12.5, 6.25 and 0% of the recommended application rate of each pesticide. The tested pesticides were classified in four scoring categories based on reduction in mycelial growth and sporulation. All pesticides, herbicides and fungicides tested had fungistatic effects of varying intensity, depending on their rate in the medium, on B. bassiana. The most inhibitory herbicides were flurochloridone and prosulfocarb, and fluazinam and copper(II) hydroxide were most inhibitory among the fungicides, while the least inhibitory were isoxaflutole and chlorothalonil. Sporulation and conidial germination of B. bassiana were significantly inhibited by all tested pesticides compared with the control treatment. Flurochloridone, foramsulfuron, prosulfocarb and copper(II) hydroxide inhibited sporulation entirely at 100% rate (99-100% inhibition), and the lowest inhibition was shown by fluazifop-P-butyl (22%) and metiram (33%). At 100% dosage, all herbicides in the test showed a high inhibitory effect on conidial germination. Conidial germination inhibition ranged from 82% with isoxaflutole to 100% with fluorochloridone, pendimethalin and prosulfocarb. At 200% dosage, inhibition rates even increased (96-100%). All 12 pesticides tested had a fungistatic effect on B. bassiana of varying intensity, depending on the pesticide and its concentration. B. bassiana is highly affected by some herbicides and fungicides even at very low rates. Flurochloridone, foramsulfuron

  17. Perspective Taking in Workplaces

    Directory of Open Access Journals (Sweden)

    Zappalà Salvatore

    2014-07-01

    Full Text Available Workplaces are often described as places in which individuals are motivated by their self-interests and in which negative events like time pressure, anxiety, conflict with co-workers, miscomprehensions, difficulties in solving problems, not-transmitted or not-exchanged information that lead to mistakes, and in some cases to injuries, stress or control, are part of everyday life (Dormann & Zapf, 2002; Schabracq, Winnubst and Cooper, 2003. Such situations are often the result of the limited comprehension of needs, skills, or information available to colleagues, supervisors, subordinates, clients or providers. However, workplaces are also places in which employees take care of clients, support colleagues and subordinates (Rhoades & Eisenberger, 2002, are enthusiastic about their job (Bakker et al., 2008, are motivated by leaders that encourage employees to transcend their own self-interests for the good of the group or the organization and provide them with the confidence to perform beyond expectations (Bass, 1997. Thus positive relationships at work are becoming a new interdisciplinary domain of inquiry (Dutton & Ragins, 2006. Within this positive relationships framework, in this paper we focus on a positive component of workplaces, and particularly on an individual cognitive and emotional process that has an important role in the workplace because it facilitates interpersonal relations and communications: it is the perspective taking process. In order to describe perspective taking, we will refer to some empirical studies and particularly to the review published by Parker, Atkins and Axtell in 2008 on the International Review of Industrial and Organizational Psychology.

  18. How Does Photosynthesis Take Place in Our Oceans?

    OpenAIRE

    Ghosh, Tonmoy; Mishra, Sandhya

    2017-01-01

    The food we eat ultimately comes from plants, either directly or indirectly. The importance of plants as the global kitchen can never be underestimated. Plants “eat” sunlight and carbon dioxide to produce their own food and food for the millions of other organisms dependent on them. A molecule, chlorophyll (Chl), is crucial for this process, since it absorbs sunlight. However, the way land plants produce their food is very different from the way plants in the oceans produce their food. Since ...

  19. BP-Mobil partnership. The common network takes place

    International Nuclear Information System (INIS)

    Anon.

    1997-01-01

    After the partnership between BP and Mobil was signed, the program of transformation of the petrol stations network started in November 1996 in the UK and concern 3300 stations in Europe and 800 stations in France. About 9100 stations will be transformed by the end of 1998. BP France is the operator for petroleum products (petrol, fuel, bitumens, LPG..) with a 70% share holding (30% for Mobil) while Mobil is the major shareholder (51%) for the lubricants and special products activities. The chemical, aviation nd maritime activities are not concerned. Thanks to the fusion of their down-file activities in Europe, the benefits of the partnership should reach 600 to 700 million of US Dollars each year. However the restructuring cost should reach 740 millions of US Dollars in two years, which doubles the initial estimation. Short paper. (J.S.)

  20. Using Personalized Education to Take the Place of Standardized Education

    Science.gov (United States)

    Gao, Pengyu

    2014-01-01

    Economic model has been greatly shifted from labor demanding to innovation demanding, which requires education system has to produce creative people. This paper illustrates how traditional education model accrued and developed based on satisfying the old economic model for labor demanding but did not meet the new social requirement for innovation…

  1. Does the measurement take place when nobody observes it

    Energy Technology Data Exchange (ETDEWEB)

    Gurvitz, Shmuel [Department of Particle Physics and Astrophysics, Weizmann Institute of Science, Rehovot (Israel); Beijing Computational Science Research Center, Beijing (China)

    2017-06-15

    We consider non-selective continuous measurements of a particle tunneling to a reservoir of finite band-width (Λ). The particle is continuously monitored by frequent projective measurements (''quantum trajectory''), separated by a time-interval τ. A simple analytical expression for the decay rate has been obtained. For Markovian reservoirs (Λ → ∞), no effect of the measurements is found. Otherwise for a finite Λ, the decay rate always depends on the measurement time τ. This result is compared with alternative calculations, with no intermediate measurements, but when the measurement device is included in the Schroedinger evolution. We found that the detector affects the system by the decoherence rate (Γ{sub d}), related to the detector's signal. Although both treatments are different, the final results become very close for τ = 2/Γ{sub d}. This τ corresponds to the minimal time for which the detector's signal can be distinguished by an ''observer''. This indicates a fundamental role of information in quantum motion and can be used for the extension of the quantum trajectory method for non-Markovian environments. (copyright 2016 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  2. I could never take the place of your man

    DEFF Research Database (Denmark)

    Andersen, Poul Houman; Munksgaard, Kristin B.

    We explore the dynamic tensions between the strategic change intentions by business actors and the rigidities of routines in business networks. We capture strategic change acts using the recent advances in the literature on network pictures as way to understand the value creation logics of manage...

  3. Application of Two-Stage Cultivation for Exploring the Nutritional Requirements for Sporulation of Three Biocontrol Fungi

    Directory of Open Access Journals (Sweden)

    Li Gao

    2015-01-01

    Full Text Available Fungicide was an important part in mycopesticides, which play an important role in pest management, while their mass production and commercialization faced problem. We found that the nutrition for mycelia growth and sporulation differences a lot. So, we developed “two-step method” to define the nutrition for sporulation in this paper. The results indicated that the novel method led to a great increase of spore yields for Beauveria bassiana (IBC1201, Lecanicillium lecanii (CA-1-G, and Pochonia chlamydosporia (HSY-12-14, respectively, of about 100, 2, and 16 times and, also reduced the cycle of mass production to 1/3 compared with common time for culturing.

  4. [Role of the ribosomes in controlling cellular differentiation and secondary metabolism in sporulating bacteria. I. Sporogenesis, antibiotic formation and the proteolytic activity of streptomycin-resistant mutants].

    Science.gov (United States)

    Lukin, A A; Korolev, V I

    1981-01-01

    A ribosomal mutant Bacillus subtilis IG1 resistant to 100 mkg/ml of streptomycin was isolated. The strA mutation is cotransduced with the cysA gene and, consequently, maps in the ribosomal cluster. The mutation does not influence cell division but does reduce a level of sporulation as well as its antibiotic and proteolytic activity. Involvement of ribosomes in the control of sporulation and secondary metabolism of spore forming bacteria is discussed.

  5. Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis.

    OpenAIRE

    Webb, C D; Decatur, A; Teleman, A; Losick, R

    1995-01-01

    We report the use of the green fluorescent protein (GFP) of Aequorea victoria to visualize cell-specific gene expression and protein subcellular localization during sporulation in Bacillus subtilis. Sporangia bearing the gene (gfp) for the green fluorescent protein fused to genes under the control of the sporulation transcription factor sigma F exhibited a forespore-specific pattern of fluorescence. Forespore-specific fluorescence could be detected with fusions to promoters that are utilized ...

  6. Les bactéries sporulées dans les conserves de légumes (petits pois ...

    African Journals Online (AJOL)

    Mots clés : Conserves végétales - Petits pois- Bactéries sporulées - Identification biochimique. For a consumer more and more aware about the risks for his health, regarding the food he buys, the aim of this study is to verify the hygienic quality of ten batches, each one contains five green peas cans, normal and identical.

  7. Dynamic sporulation gene co-expression networks for Bacillus subtilis 168 and the food-borne isolate Bacillus amyloliquefaciens: a transcriptomic model.

    Science.gov (United States)

    Omony, Jimmy; de Jong, Anne; Krawczyk, Antonina O; Eijlander, Robyn T; Kuipers, Oscar P

    2018-02-09

    Sporulation is a survival strategy, adapted by bacterial cells in response to harsh environmental adversities. The adaptation potential differs between strains and the variations may arise from differences in gene regulation. Gene networks are a valuable way of studying such regulation processes and establishing associations between genes. We reconstructed and compared sporulation gene co-expression networks (GCNs) of the model laboratory strain Bacillus subtilis 168 and the food-borne industrial isolate Bacillus amyloliquefaciens. Transcriptome data obtained from samples of six stages during the sporulation process were used for network inference. Subsequently, a gene set enrichment analysis was performed to compare the reconstructed GCNs of B. subtilis 168 and B. amyloliquefaciens with respect to biological functions, which showed the enriched modules with coherent functional groups associated with sporulation. On basis of the GCNs and time-evolution of differentially expressed genes, we could identify novel candidate genes strongly associated with sporulation in B. subtilis 168 and B. amyloliquefaciens. The GCNs offer a framework for exploring transcription factors, their targets, and co-expressed genes during sporulation. Furthermore, the methodology described here can conveniently be applied to other species or biological processes.

  8. Salt-sensitivity of σH and Spo0A prevents sporulation of Bacillus subtilis at high osmolarity avoiding death during cellular differentiation

    Science.gov (United States)

    Widderich, Nils; Rodrigues, Christopher D.A.; Commichau, Fabian M.; Fischer, Kathleen E.; Ramirez-Guadiana, Fernando H.; Rudner, David Z.; Bremer, Erhard

    2016-01-01

    Summary The spore-forming bacterium Bacillus subtilis frequently experiences high osmolarity as a result of desiccation in the soil. The formation of a highly desiccation-resistant endospore might serve as a logical osmostress escape route when vegetative growth is no longer possible. However, sporulation efficiency drastically decreases concomitant with an increase in the external salinity. Fluorescence microscopy of sporulation-specific promoter fusions to gfp revealed that high salinity blocks entry into the sporulation pathway at a very early stage. Specifically, we show that both Spo0A- and SigH-dependent transcription are impaired. Furthermore, we demonstrate that the association of SigH with core RNA polymerase is reduced under these conditions. Suppressors that modestly increase sporulation efficiency at high salinity map to the coding region of sigH and in the regulatory region of kinA, encoding one the sensor kinases that activates Spo0A. These findings led us to discover that B. subtilis cells that overproduce KinA can bypass the salt-imposed block in sporulation. Importantly, these cells are impaired in the morphological process of engulfment and late forespore gene expression and frequently undergo lysis. Altogether our data indicate that B. subtilis blocks entry into sporulation in high-salinity environments preventing commitment to a developmental program that it cannot complete. PMID:26712348

  9. Impacts of sporulation temperature, exposure to compost matrix and temperature on survival of Bacillus cereus spores during livestock mortality composting.

    Science.gov (United States)

    Stanford, K; Reuter, T; Gilroyed, B H; McAllister, T A

    2015-04-01

    To investigate impact of sporulation and compost temperatures on feasibility of composting for disposal of carcasses contaminated with Bacillus anthracis. Two strains of B. cereus, 805 and 1391, were sporulated at either 20 or 37°C (Sporulation temperature, ST) and 7 Log10 CFU g(-1) spores added to autoclaved manure in nylon bags (pore size 50 μm) or in sealed vials. Vials and nylon bags were embedded into compost in either a sawdust or manure matrix each containing 16 bovine mortalities (average weight 617 ± 33 kg), retrieved from compost at intervals over 217 days and survival of B. cereus spores assessed. A ST of 20°C decreased spore survival by 1·4 log10 CFU g(-1) (P Compost temperatures >55°C reduced spore survival (P compost temperatures were key factors influencing survival of B. cereus spores in mortality compost. Composting may be most appropriate for the disposal of carcasses infected with B. anthracis at ambient temperatures ≤20°C under thermophillic composting conditions (>55°C). © 2015 The Society for Applied Microbiology.

  10. Metabolic alterations in broiler chickens experimentally infected with sporulated oocysts of Eimeria maxima

    Directory of Open Access Journals (Sweden)

    Fagner Luiz da Costa Freitas

    Full Text Available Metabolic and morphometric alterations of the duodenal villi caused by parasitism of chickens by Eimeria maxima were evaluated, using 100 male Cobb birds, randomly distributed into two groups (control and infected. The infected group was inoculated with 0.5 ml of a solution containing 5×103 sporulated oocysts of Eimeria maxima. Ten birds per sample were sacrificed on the 6th, 11th, 22nd and 41st days post-infection (dpi. In order to evaluate the alterations, samples of duodenum, jejunum and ileum fragments were collected after necropsy for histological analysis. Villus biometry was determined by means of a slide graduated in microns that was attached to a binocular microscope. To evaluate the biochemical data, 5 ml of blood were sampled from the birds before sacrifice. The statistical analyses were performed using the GraphPad 5 statistical software for Windows. Tukey's multiple comparison test (p <0.05 was performed for the different dpi's and the unpaired t test for the difference between the groups. Infection by E. maxima causes both qualitative and quantitative alterations to the structure of the intestinal villi, thereby interfering with the absorption of nutrients such as calcium, phosphorus, magnesium, protein and lipids, with consequent reductions in the birds' weights.

  11. Metabolic alterations in broiler chickens experimentally infected with sporulated oocysts of Eimeria maxima.

    Science.gov (United States)

    Freitas, Fagner Luiz da Costa

    2014-01-01

    Metabolic and morphometric alterations of the duodenal villi caused by parasitism of chickens by Eimeria maxima were evaluated, using 100 male Cobb birds, randomly distributed into two groups (control and infected). The infected group was inoculated with 0.5 ml of a solution containing 5 × 10³ sporulated oocysts of Eimeria maxima. Ten birds per sample were sacrificed on the 6th, 11th, 22nd and 41st days post-infection (dpi). In order to evaluate the alterations, samples of duodenum, jejunum and ileum fragments were collected after necropsy for histological analysis. Villus biometry was determined by means of a slide graduated in microns that was attached to a binocular microscope. To evaluate the biochemical data, 5 ml of blood were sampled from the birds before sacrifice. The statistical analyses were performed using the GraphPad 5 statistical software for Windows. Tukey's multiple comparison test (p <0.05) was performed for the different dpi's and the unpaired t test for the difference between the groups. Infection by E. maxima causes both qualitative and quantitative alterations to the structure of the intestinal villi, thereby interfering with the absorption of nutrients such as calcium, phosphorus, magnesium, protein and lipids, with consequent reductions in the birds' weights.

  12. Cgl-SLT2 is required for appressorium formation, sporulation and pathogenicity in Colletotrichum gloeosporioide

    Directory of Open Access Journals (Sweden)

    H.Y. Yong

    2013-12-01

    Full Text Available The mitogen-activated protein (MAP kinase pathways has been implicated in the pathogenicity of various pathogenic fungi and plays important roles in regulating pathogenicity-related morphogenesis. This work describes the isolation and characterization of MAP kinase gene, Cgl-SLT2, from Colletotrichum gloeosporioides. A DNA sequence, including 1,633 bp of Cgl-SLT2 open-reading frame and its promoter and terminator regions, was isolated via DNA walking and cloned. To analyze gene function, a gene disruption cassette containing hygromycin-resistant gene was constructed, and Cgl-SLT2 was inactivated via gene deletion. Analysis on Cgl-slt2 mutant revealed a defect in vegetative growth and sporulation as compared to the wild-type strain. When grown under nutrient-limiting conditions, hyperbranched hyphal morphology was observed in the mutant. Conidia induction for germination on rubber wax-coated hard surfaces revealed no differences in the percentage of conidial germination between the wild-type and Cgl-slt2 mutant. However, the percentage of appressorium formation in the mutant was greatly reduced. Bipolar germination in the mutant was higher than in the wild-type at 8-h post-induction. A pathogenicity assay revealed that the mutant was unable to infect either wounded or unwounded mangoes. These results suggest that the Cgl-SLT2 MAP kinase is required for C. gloeosporioides conidiation, polarized growth, appressorium formation and pathogenicity.

  13. MrSkn7 controls sporulation, cell wall integrity, autolysis, and virulence in Metarhizium robertsii.

    Science.gov (United States)

    Shang, Yanfang; Chen, Peilin; Chen, Yixiong; Lu, Yuzhen; Wang, Chengshu

    2015-04-01

    Two-component signaling pathways generally include sensor histidine kinases and response regulators. We identified an ortholog of the response regulator protein Skn7 in the insect-pathogenic fungus Metarhizium robertsii, which we named MrSkn7. Gene deletion assays and functional characterizations indicated that MrSkn7 functions as a transcription factor. The MrSkn7 null mutant of M. robertsii lost the ability to sporulate and had defects in cell wall biosynthesis but was not sensitive to oxidative and osmotic stresses compared to the wild type. However, the mutant was able to produce spores under salt stress. Insect bioassays using these spores showed that the virulence of the mutant was significantly impaired compared to that of the wild type due to the failures to form the infection structure appressorium and evade host immunity. In particular, deletion of MrSkn7 triggered cell autolysis with typical features such as cell vacuolization, downregulation of repressor genes, and upregulation of autolysis-related genes such as extracellular chitinases and proteases. Promoter binding assays confirmed that MrSkn7 could directly or indirectly control different putative target genes. Taken together, the results of this study help us understand the functional divergence of Skn7 orthologs as well as the mechanisms underlying the development and control of virulence in insect-pathogenic fungi. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  14. Roles of the DYRK kinase Pom2 in cytokinesis, mitochondrial morphology, and sporulation in fission yeast.

    Directory of Open Access Journals (Sweden)

    Pengcheng Wu

    Full Text Available Pom2 is predicted to be a dual-specificity tyrosine-phosphorylation regulated kinase (DYRK related to Pom1 in Schizosaccharomyces pombe. DYRKs share a kinase domain capable of catalyzing autophosphorylation on tyrosine and exogenous phosphorylation on serine/threonine residues. Here we show that Pom2 is functionally different from the well-characterized Pom1, although they share 55% identity in the kinase domain and the Pom2 kinase domain functionally complements that of Pom1. Pom2 localizes to mitochondria throughout the cell cycle and to the contractile ring during late stages of cytokinesis. Overexpression but not deletion of pom2 results in severe defects in cytokinesis, indicating that Pom2 might share an overlapping function with other proteins in regulating cytokinesis. Gain and loss of function analyses reveal that Pom2 is required for maintaining mitochondrial morphology independently of microtubules. Intriguingly, most meiotic pom2Δ cells form aberrant asci with meiotic and/or forespore membrane formation defects. Taken together, Pom2 is a novel DYRK kinase involved in regulating cytokinesis, mitochondrial morphology, meiosis, and sporulation in fission yeast.

  15. Assessing the Impact of Germination and Sporulation Conditions on the Adhesion of Bacillus Spores to Glass and Stainless Steel by Fluid Dynamic Gauging.

    Science.gov (United States)

    Xu Zhou, Ke; Li, Nan; Christie, Graham; Wilson, D Ian

    2017-11-01

    The adhesion of spores of 3 Bacillus species with distinctive morphologies to stainless steel and borosilicate glass was studied using the fluid dynamic gauging technique. Marked differences were observed between different species of spores, and also between spores of the same species prepared under different sporulation conditions. Spores of the food-borne pathogen B. cereus were demonstrated to be capable of withstanding shear stresses greater than 1500 Pa when adhered to stainless steel, in contrast to spores of Bacillus subtilis and Bacillus megaterium, which detached in response to lower shear stress. An extended DLVO model was shown to be capable of predicting the relative differences in spore adhesion between spores of different species and different culture conditions, but did not predict absolute values of force of adhesion well. Applying the model to germinating spores showed a significant reduction in adhesion force shortly after triggering germination, indicating a potential strategy to achieve enhanced removal of spores from surfaces in response to shear stress, such as during cleaning-in-place procedures. Spore-forming bacteria are a concern to the food industry because they have the potential to cause food-borne illness and product spoilage, while being strongly adhesive to processing surfaces and resistant to cleaning-in-place procedures. This work is of significance to the food processors and manufacturers because it offers insight to the properties of spore adhesion and identifies a potential strategy to facilitate the removal of spores during cleaning procedures. © 2017 The Authors. Journal of Food Science published by Wiley Periodicals, Inc. on behalf of Institute of Food Technologists.

  16. Determining the Pathogenic Potential of Non-sporulating Molds Isolated from Cutaneous Specimens.

    Science.gov (United States)

    Jeyaprakasam, Nantha Kumar; Razak, Mohd Fuat Abdul; Ahmad, Noor Azimah Binti; Santhanam, Jacinta

    2016-06-01

    Although non-sporulating molds (NSM) are frequently isolated from patients and have been recognized as agents of pulmonary disease, their clinical significance in cutaneous specimens is relatively unknown. Therefore, this study aimed to identify NSM and to determine the keratinolytic activity of isolates from cutaneous sites. NSM isolates from clinical specimens such as skin, nail, and body fluids were identified based on their ribosomal DNA sequences. Of 17 NSM isolates (7 Ascomycota, 10 Basidiomycota), eleven were identified to species level while five were identified to the genus level. These include Schizophyllum commune, a known human pathogen, Phoma multirostrata, a plant pathogen, and Perenniporia tephropora, a saprophyte. To determine fungal pathogenicity, keratinolytic activity, a major virulence factor, was evaluated ex vivo using human nail samples by measuring dye release from keratin azure, for NSM along with pathogens (Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis and Fusarium spp.) and nonpathogenic (endophyte) fungi for comparison. This study showed that pathogenic fungi had the highest keratinolytic activity (7.13 ± 0.552 keratinase units) while the nonpathogenic endophytes had the lowest activity (2.37 ± 0.262 keratinase units). Keratinolytic activity of two Ascomycota NSM (Guignardia mangiferae and Hypoxylon sp.) and one Basidiomycota NSM (Fomitopsis cf. meliae) was equivalent to that of pathogenic fungi, while Xylaria feejeensis showed significantly higher activity (p < 0.05) than nonpathogenic endophytes. These results indicate that the pathogenic ability of NSM is species dependent; clinical isolates, especially more frequently isolated species, may be involved in disease etiology.

  17. Transience and Place: Exploring Tourists' Experiences of Place

    Directory of Open Access Journals (Sweden)

    Jens Kristian Steen Jacobsen

    1998-02-01

    Full Text Available This paper explores tourists' transient place experiences. The focus is on what is commonly called sightseeing, confined mainly to experiences of locations not previously visited. This type of travel experience, seeing actual places or objects for one's self and taking photographs, is regarded here as an important ritual in modernity. With reference to the concept of adventurer, the paper attempts to describe various aspects of novel travel experiences. For instance, ephemeral tourist sensations of place are compared to «love at first sight», a blissful experience of meeting with a place to which one feels strongly attracted. While transient encounters may also provide access to dreamlike place experiences, adventurous tourists are always in danger of being caught up in other roles, or of becoming embroiled in a new everyday life with accompanying routines. By way of conclusion, it is proposed that the restrictions on time inherent in today's holiday tours usually result in transient place experiences as the only alternative for those desiring to gain first-time impressions of several places or larger geographic areas.

  18. Increase of the Bacillus thuringiensis secreted toxicity against lepidopteron larvae by homologous expression of the vip3LB gene during sporulation stage.

    Science.gov (United States)

    Sellami, Sameh; Jamoussi, Kaïs; Dabbeche, Emna; Jaoua, Samir

    2011-09-01

    The Vegetative insecticidal Vip3A proteins display a wide range of insecticidal spectrum against several agricultural insect pests. The fact that the expression of vip3 genes occurs only during the vegetative growth phase of Bacillus thuringiensis is a limiting factor in term of production level. Therefore, extending the synthesis of the Vip proteins to the sporulation phase is a good alternative to reach high levels of toxin synthesis. In this study, we have demonstrated that the maximal production of the secreted Vip3LB (also called Vip3Aa16) during the growth of the wild-type strain B. thuringiensis BUPM 95 is reached at the end of the vegetative growth phase, and that the protein remains relatively stable in the culture supernatant during the late sporulation stages. The vip3LB gene was cloned and expressed under the control of the sporulation dependant promoters BtI and BtII in B. thuringiensis BUPM 106 (Vip3(-)) and BUPM 95 (Vip3(+)) strains. The examination of the culture supernatants during the sporulation phase evidenced the synthesis of Vip3LB and its toxicity against the second-instars larvae of the Lepidopteron insect Spodoptera littoralis for the recombinant BUPM 106. Moreover, there was an increase of the Vip3LB synthesis level and an enhancement of the oral toxicity for the recombinant BUPM 95 resulting from the expression of the vip3LB gene during both the vegetative and sporulation phases and the relative stability of the Vip3LB protein.

  19. The comER Gene Plays an Important Role in Biofilm Formation and Sporulation in both Bacillus subtilis and Bacillus cereus.

    Science.gov (United States)

    Yan, Fang; Yu, Yiyang; Wang, Luyao; Luo, Yuming; Guo, Jian-Hua; Chai, Yunrong

    2016-01-01

    Bacteria adopt alternative cell fates during development. In Bacillus subtilis, the transition from planktonic growth to biofilm formation and sporulation is controlled by a complex regulatory circuit, in which the most important event is activation of Spo0A, a transcription factor and a master regulator for genes involved in both biofilm formation and sporulation. In B. cereus, the regulatory pathway controlling biofilm formation and cell differentiation is much less clear. In this study, we show that a novel gene, comER, plays a significant role in biofilm formation as well as sporulation in both B. subtilis and B. cereus. Mutations in the comER gene result in defects in biofilm formation and a delay in spore formation in the two Bacillus species. Our evidence supports the idea that comER may be part of the regulatory circuit that controls Spo0A activation. comER likely acts upstream of sda, a gene encoding a small checkpoint protein for both sporulation and biofilm formation, by blocking the phosphor-relay and thereby Spo0A activation. In summary, our studies outlined a conserved, positive role for comER, a gene whose function was previously uncharacterized, in the regulation of biofilm formation and sporulation in the two Bacillus species.

  20. The comER Gene Plays an Important Role in Biofilm Formation and Sporulation in both Bacillus subtilis and Bacillus cereus

    Science.gov (United States)

    Yan, Fang; Yu, Yiyang; Wang, Luyao; Luo, Yuming; Guo, Jian-hua; Chai, Yunrong

    2016-01-01

    Bacteria adopt alternative cell fates during development. In Bacillus subtilis, the transition from planktonic growth to biofilm formation and sporulation is controlled by a complex regulatory circuit, in which the most important event is activation of Spo0A, a transcription factor and a master regulator for genes involved in both biofilm formation and sporulation. In B. cereus, the regulatory pathway controlling biofilm formation and cell differentiation is much less clear. In this study, we show that a novel gene, comER, plays a significant role in biofilm formation as well as sporulation in both B. subtilis and B. cereus. Mutations in the comER gene result in defects in biofilm formation and a delay in spore formation in the two Bacillus species. Our evidence supports the idea that comER may be part of the regulatory circuit that controls Spo0A activation. comER likely acts upstream of sda, a gene encoding a small checkpoint protein for both sporulation and biofilm formation, by blocking the phosphor-relay and thereby Spo0A activation. In summary, our studies outlined a conserved, positive role for comER, a gene whose function was previously uncharacterized, in the regulation of biofilm formation and sporulation in the two Bacillus species. PMID:27446060

  1. Place Branding in Systems of Place

    DEFF Research Database (Denmark)

    Zenker, Sebastian; Andéhn, Mikael

    2015-01-01

    be lost and its meaning relies on a reading of the brand by anyone receiving its message, the control that can be exerted over which meaning is attributed to a company brand appears almost absolute when compared to a place brand. A place is a brand often long before it is formally branded. A place brand.......g. the European Union or Africa). Using the example of nation branding for Sudan and Slovenia, one can identify supranational places such as “sub-Saharan Africa” or “Eastern Europe”, carrying their own highly salient and often negative meaning in much of the Western world. We explore how association to a system...... of place is thought to influence the prospects of branding campaigns and introduce and discuss how reassociation can serve as means of mitigation of negative supranational belongingness. In doing so, we discuss also the challenges of interregional branding in this regard, where perceptions of two (or more...

  2. Kinetic modeling of sporulation and product formation in stationary phase by Bacillus coagulans RK-02 vis-à-vis other Bacilli.

    Science.gov (United States)

    Das, Subhasish; Sen, Ramkrishna

    2011-10-01

    A logistic kinetic model was derived and validated to characterize the dynamics of a sporogenous bacterium in stationary phase with respect to sporulation and product formation. The kinetic constants as determined using this model are particularly important for describing intrinsic properties of a sporogenous bacterial culture in stationary phase. Non-linear curve fitting of the experimental data into the mathematical model showed very good correlation with the predicted values for sporulation and lipase production by Bacillus coagulans RK-02 culture in minimal media. Model fitting of literature data of sporulation and product (protease and amylase) formation in the stationary phase by some other Bacilli and comparison of the results of model fitting with those of Bacillus coagulans helped validate the significance and robustness of the developed kinetic model. Copyright © 2011 Elsevier Ltd. All rights reserved.

  3. THE EFFECT OF GROWTH PARAMETERS ON THE ANTIBIOTIC ACTIVITY AND SPORULATION IN BACILLUS SPP. ISOLATED FROM SOIL

    Directory of Open Access Journals (Sweden)

    Alev Usta

    2013-04-01

    Full Text Available Fifty-two Bacillus strains, which were isolated from different soil samples, were screened for antibiotic properties. The Bacillus strains were checked for antibacterial properties by the cross-streak method against 5 test pathogens, and 25 Bacillus strains had an effect on the test microorganisms. One strain of Bacillus, which exhibited the largest inhibition zone (25 mm against Shigella sonnei, was named Bacillus sp. EA62. The antibacterial activity from Bacillus sp. EA62 was tested in six different culture media against Shigella sonnei using the agar well diffusion method. The best activity medium was selected and used for further studies. The influence of the incubation period, pH, and different glucose and nitrogen concentrations on the antibacterial activity was studied. The optimal conditions for the strongest antibiotic activity were found to be 72 hours (18 mm, pH 7.5 (23 mm, 3% glucose (25 mm, and 0.3% nitrogen concentration (23 mm. Additionally, the relationship between the antibiotic activity and sporulation was investigated. Accordingly, it was determined that the increase of the activity paralleled sporulation.

  4. Antimicrobial copper alloy surfaces are effective against vegetative but not sporulated cells of gram-positive Bacillus subtilis

    Science.gov (United States)

    San, Kaungmyat; Long, Janet; Michels, Corinne A; Gadura, Nidhi

    2015-01-01

    This study explores the role of membrane phospholipid peroxidation in the copper alloy mediated contact killing of Bacillus subtilis, a spore-forming gram-positive bacterial species. We found that B. subtilis endospores exhibited significant resistance to copper alloy surface killing but vegetative cells were highly sensitive to copper surface exposure. Cell death and lipid peroxidation occurred in B. subtilis upon copper alloy surface exposure. In a sporulation-defective strain carrying a deletion of almost the entire SpoIIA operon, lipid peroxidation directly correlated with cell death. Moreover, killing and lipid peroxidation initiated immediately and at a constant rate upon exposure to the copper surface without the delay observed previously in E. coli. These findings support the hypothesis that membrane lipid peroxidation is the initiating event causing copper surface induced cell death of B. subtilis vegetative cells. The findings suggest that the observed differences in the kinetics of copper-induced killing compared to E. coli result from differences in cell envelop structure. As demonstrated in E. coli, DNA degradation was shown to be a secondary effect of copper exposure in a B. subtilis sporulation-defective strain. PMID:26185055

  5. Alternative Sigma Factors SigF, SigE, and SigG Are Essential for Sporulation in Clostridium botulinum ATCC 3502

    OpenAIRE

    Kirk, David G.; Zhang, Zhen; Korkeala, Hannu; Lindström, Miia

    2014-01-01

    Clostridium botulinum produces heat-resistant endospores that may germinate and outgrow into neurotoxic cultures in foods. Sporulation is regulated by the transcription factor Spo0A and the alternative sigma factors SigF, SigE, SigG, and SigK in most spore formers studied to date. We constructed mutants of sigF, sigE, and sigG in C. botulinum ATCC 3502 and used quantitative reverse transcriptase PCR and electron microscopy to assess their expression of the sporulation pathway on transcription...

  6. Place branding, embeddedness and endogenous rural development

    NARCIS (Netherlands)

    Donner, Mechthild; Horlings, Lummina; Fort, Fatiha; Vellema, Sietze

    2017-01-01

    This article deals with place branding on the regional scale, in the rural context of food and tourism networks in Europe. Place branding is linked to the concepts of endogenous rural development, territory and embeddedness, by analysing how the valorisation of specific rural assets takes shape.

  7. Populated Places of Iowa

    Data.gov (United States)

    Iowa State University GIS Support and Research Facility — This coverage contains points that represent populated places, ie. cities, towns, villages or any other named place where people live. The coverage was developed...

  8. The effect of metal ions commonly present in food on gene expression of sporulating Bacillus subtilis cells in relation to spore wet heat resistance.

    NARCIS (Netherlands)

    Oomes, S.J.C.M.; Brul, S.

    2004-01-01

    Bacillus subtilis is a food spoilage spore-forming bacterium. The spores can be very heat-resistant and may cause problems in the production of foods. Varying the metal concentration in the sporulation media is known to influence the heat resistance of the spores. The effect of changing the metal

  9. Dynamic sporulation gene co-expression networks for Bacillus subtilis 168 and the food-borne isolate Bacillus amyloliquefaciens : a transcriptomic model

    NARCIS (Netherlands)

    Omony, Jimmy; de Jong, Anne; Krawczyk, Antonina O; Eijlander, Robyn T; Kuipers, Oscar P

    2018-01-01

    Sporulation is a survival strategy, adapted by bacterial cells in response to harsh environmental adversities. The adaptation potential differs between strains and the variations may arise from differences in gene regulation. Gene networks are a valuable way of studying such regulation processes and

  10. A Gene Expressed during Sexual and Asexual Sporulation in Phytophthora infestans is a Member of the Puf Family of Translational Regulators

    DEFF Research Database (Denmark)

    Cvitanich, Cristina; Judelson, Howard S.

    2003-01-01

    A gene from Phytophthora infestans that was previously identified as being induced during the development of sexual spores was also found to be active during asexual sporulation. The gene, M90, was expressed as a 3.1-kb primary transcript containing two introns and was predicted to encode a member...

  11. Ebishushani: people poses places

    NARCIS (Netherlands)

    Andrea Stultiens

    2014-01-01

    Ebifananyi II – People Poses Places Andrea Stultiens People Poses Places is the second part of Ebifananyi, a book series that visualises historical Ugandan photo collections. In People Poses Places we delve into the archive of the photographer Musa Katuramu. In the mid 1930s, teacher and carpenter

  12. Compensated takings and negotiated solutions

    International Nuclear Information System (INIS)

    Thompson, R.P.

    1993-01-01

    More than 30 year ago, the USA began protecting its environment with regulatory laws. However, this approach has progressed to the point where our economic health may have needlessly incurred long-term damage. The article explores the implications of a resurgent demand for legal guarantees for compensation when environmental regulations take individual property. What will happen when a price tag is attached to new or existing environmental laws? Some predict dire results; however, a more balanced and efficient policy may lie ahead. The discussion explores the implications of recent rulings, describes possible outcomes, and offers several alternatives to the traditional lobby-and-appeal tactics used in setting environmental policy. The authors conclude that as the concept of a regulatory taking is restored, more innovative negotiated solutions will take place between competing interests as they are given more equal legal footing

  13. Take Charge. Take the Test. PSA (:30)

    Centers for Disease Control (CDC) Podcasts

    2012-03-07

    As part of the Take Charge. Take the Test. campaign, this 30 second PSA encourages African American women to get tested for HIV. Locations for a free HIV test can be found by visiting hivtest.org/takecharge or calling 1-800-CDC-INFO (1-800-232-4636).  Created: 3/7/2012 by National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention (NCHHSTP).   Date Released: 3/7/2012.

  14. Where is 'place' in Aging in place?

    DEFF Research Database (Denmark)

    Blaakilde, Anne Leonora

    2015-01-01

    The aim of this article is to contribute a transnational perspective to the field of environmental gerontology and the concept of aging in place. Seniors from the northern hemisphere, among them Danish citizens, are increasingly adapting to transnational lives as they move to warmer climates...

  15. The importance of places and place branding

    NARCIS (Netherlands)

    Kovács, Z.; Musterd, S.; Musterd, S.; Kovács, Z.

    2013-01-01

    In the analytical part of this study, we highlight that, despite globalisation and growing uniformity, there is still an important role for place itself in the location decisions of economic players. Part III of this volume deals with this. Even though city-regions across the world have become

  16. Place-Specific Computing

    DEFF Research Database (Denmark)

    Messeter, Jörn

    2009-01-01

    An increased interest in the notion of place has evolved in interaction design based on the proliferation of wireless infrastructures, developments in digital media, and a ‘spatial turn’ in computing. In this article, place-specific computing is suggested as a genre of interaction design...... that addresses the shaping of interactions among people, place-specific resources and global socio-technical networks, mediated by digital technology, and influenced by the structuring conditions of place. The theoretical grounding for place-specific computing is located in the meeting between conceptions...... examples of place-specific computing are presented from a series of pilot studies, conducted in close collaboration with design students in Malmö, Berlin, Cape Town and Rome, that generated 36 design concepts in the genre. Reflecting on these examples, issues in the design of place-specific computing...

  17. Morphological and genetic characterization of group I Clostridium botulinum type B strain 111 and the transcriptional regulator spoIIID gene knockout mutant in sporulation.

    Science.gov (United States)

    Hosomi, Koji; Kuwana, Ritsuko; Takamatsu, Hiromu; Kohda, Tomoko; Kozaki, Shunji; Mukamoto, Masafumi

    2015-06-01

    Clostridium botulinum is a heat-resistant spore-forming bacterium that causes the serious paralytic illness botulism. Heat-resistant spores may cause food sanitation hazards and sporulation plays a central role in the survival of C. botulinum. We observed morphological changes and investigated the role of the transcriptional regulator SpoIIID in the sporulation of C. botulinum type B strain 111 in order to elucidate the molecular mechanism in C. botulinum. C. botulinum type B formed heat-resistant spores through successive morphological changes corresponding to those of Bacillus subtilis, a spore-forming model organism. An analysis of the spoIIID gene knockout mutant revealed that the transcriptional regulator SpoIIID contributed to heat-resistant spore formation by C. botulinum type B and activated the transcription of the sigK gene later during sporulation. Transcription of the spoIIID gene, which differed from that in B. subtilis and Clostridium difficile, was observed in the sigE gene knockout mutant of C. botulinum type B. An analysis of the sigF gene knockout mutant showed that the sporulation-specific sigma factor SigF was essential for transcription of the spoIIID gene in C. botulinum type B. These results suggest that the regulation of sporulation in C. botulinum is not similar to that in B. subtilis and other clostridia. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. Alternative sigma factors SigF, SigE, and SigG are essential for sporulation in Clostridium botulinum ATCC 3502.

    Science.gov (United States)

    Kirk, David G; Zhang, Zhen; Korkeala, Hannu; Lindström, Miia

    2014-08-01

    Clostridium botulinum produces heat-resistant endospores that may germinate and outgrow into neurotoxic cultures in foods. Sporulation is regulated by the transcription factor Spo0A and the alternative sigma factors SigF, SigE, SigG, and SigK in most spore formers studied to date. We constructed mutants of sigF, sigE, and sigG in C. botulinum ATCC 3502 and used quantitative reverse transcriptase PCR and electron microscopy to assess their expression of the sporulation pathway on transcriptional and morphological levels. In all three mutants the expression of spo0A was disrupted. The sigF and sigE mutants failed to induce sigG and sigK beyond exponential-phase levels and halted sporulation during asymmetric cell division. In the sigG mutant, peak transcription of sigE was delayed and sigK levels remained lower than that in the parent strain. The sigG mutant forespore was engulfed by the mother cell and possessed a spore coat but no peptidoglycan cortex. The findings suggest that SigF and SigE of C. botulinum ATCC 3502 are essential for early sporulation and late-stage induction of sigK, whereas SigG is essential for spore cortex formation but not for coat formation, as opposed to previous observations in B. subtilis sigG mutants. Our findings add to a growing body of evidence that regulation of sporulation in C. botulinum ATCC 3502, and among the clostridia, differs from the B. subtilis model. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

  19. Regulation of neurotoxin production and sporulation by a Putative agrBD signaling system in proteolytic Clostridium botulinum.

    Science.gov (United States)

    Cooksley, Clare M; Davis, Ian J; Winzer, Klaus; Chan, Weng C; Peck, Michael W; Minton, Nigel P

    2010-07-01

    A significant number of genome sequences of Clostridium botulinum and related species have now been determined. In silico analysis of these data revealed the presence of two distinct agr loci (agr-1 and agr-2) in all group I strains, each encoding putative proteins with similarity to AgrB and AgrD of the well-studied Staphylococcus aureus agr quorum sensing system. In S. aureus, a small diffusible autoinducing peptide is generated from AgrD in a membrane-located processing event that requires AgrB. Here the characterization of both agr loci in the group I strain C. botulinum ATCC 3502 and of their homologues in a close relative, Clostridium sporogenes NCIMB 10696, is reported. In C. sporogenes NCIMB 10696, agr-1 and agr-2 appear to form transcriptional units that consist of agrB, agrD, and flanking genes of unknown function. Several of these flanking genes are conserved in Clostridium perfringens. In agreement with their proposed role in quorum sensing, both loci were maximally expressed during late-exponential-phase growth. Modulation of agrB expression in C. sporogenes was achieved using antisense RNA, whereas in C. botulinum, insertional agrD mutants were generated using ClosTron technology. In comparison to the wild-type strains, these strains exhibited drastically reduced sporulation and, for C. botulinum, also reduced production of neurotoxin, suggesting that both phenotypes are controlled by quorum sensing. Interestingly, while agr-1 appeared to control sporulation, agr-2 appeared to regulate neurotoxin formation.

  20. My Place Is Not Your Place

    DEFF Research Database (Denmark)

    Zenker, Sebastian; Beckmann, Suzanne C.

    2013-01-01

    Purpose – Cities increasingly compete with each other for attracting tourists, investors, companies, or residents. Marketers therefore focus on establishing the city as a brand, disregarding that the perception and knowledge of a city differ dramatically between the target audiences. Hence, place...... branding should emphasize much more the perceptions of the different target groups and develop strategies for advanced place brand management. The aim of this paper is to assess the important discrepancies between the city brand perceptions of different target groups with the help of network analysis....... Design/methodology/approach – In two empirical studies, the important discrepancies between the city brand perceptions of different target groups are assessed with the help of network analysis. Study 1 consists of 40 qualitative in-depth-interviews and study 2 uses an online qualitative open...

  1. Management and communication courses – Places available

    CERN Multimedia

    2012-01-01

    There are places available in some management and communication courses taking place in the period January to March 2013.   For more information on the course, click on the course title, which will bring you to the training catalogue. You can then sign-up on-line. For advice, you can contact: Erwin Mosselmans, tel. 74125, erwin.mosselmans@cern.ch Nathalie Dumeaux, tel. 78144, nathalie.dumeaux@cern.ch Courses in English (or bilingual) Session Duration Language Availability How to get, as a supervisor, the most out of the annual interview 21 January 1 day English 2 places Project Engineering 24 and 25 January 2 days English 6 places How to get, as a supervisor, the most out of the annual interview 31 January 1 day English 6 places Conflict Resolution for Managers 19 and 20 February 2 days English One more place Project Scheduling & Costing 6 and 7 March 2 days English 2 places Communicati...

  2. Taking iron supplements

    Science.gov (United States)

    ... medlineplus.gov/ency/article/007478.htm Taking iron supplements To use the sharing features on this page, ... levels. You may also need to take iron supplements as well to rebuild iron stores in your ...

  3. Taking multiple medicines safely

    Science.gov (United States)

    ... medlineplus.gov/ency/patientinstructions/000883.htm Taking multiple medicines safely To use the sharing features on this ... directed. Why You May Need More Than One Medicine You may take more than one medicine to ...

  4. The value of place

    Science.gov (United States)

    Dentzau, Michael W.

    2014-03-01

    This commentary seeks to expand the dialogue on place-based science education presented in Katie Lynn Brkich's article, where the connections fifth grade students make between their formal earth science curriculum and their lived experiences are highlighted. The disconnect between the curriculum the students are offered and their immediate environment is clear, and we are presented with examples of how they strive to make connections between the content and what they are familiar with—namely their surroundings. "Place" is identified as a term with complex meanings and interpretations, even in the scope of place-based science education, and understanding how the term is used in any given scenario is essential to understanding the implications of place-based education. Is place used as a location, locale or a sense of place? To understand "place" is to acknowledge that for the individual, it is highly situational, cultural and personal. It is just such attributes that make place-based education appealing, and potentially powerful, pedagogically on one hand, yet complex for implementation on the other. The argument is posed that place is particularly important in the context of education about the environment, which in its simplest manifestation, connects formal science curriculum to resources that are local and tangible to students. The incorporation of place in such a framework seeks to bridge the gap between formal school science subjects and students' lived experiences, yet acknowledges the tensions that can arise between accommodating place meanings and the desire to acculturate students into the language of the scientific community. The disconnect between guiding policy frameworks and the reality of the Next Generation Science Standards is addressed opening an avenue for further discussion of the importance of socio-cultural frameworks of science learning in an ever increasing era of accountability.

  5. Technical training: places available

    CERN Multimedia

    HR Department

    2007-01-01

    CERN Technical Training: Open Courses (April - June 2007) The following course sessions are currently scheduled in the framework of the CERN Technical Training Programme 2007:   AutoCAD 2006 - niveau 1 (course in French): 25.4.- 26.4.2007 & 2.5. - 3.5.2007 (4 days in 2 modules, 5 places available) AutoCAD 2006 - niveau 1 (course in French): 27.6.- 28.6.2007 & 3.7. - 4.7.2007 (4 days in 2 modules, 5 places available) AutoCAD Mechanical 2006 (course in French) 21.6.-22.6.2007 (2 days, 8 places available) * NEW COURSE* Automate de securite S7 (course in French) 14.5.-16.5.2007 (3 days, 4 places available) * NEW COURSE* Automate de securite S7 (course in French): 9.5.-11.5.2007 (3 days, 4 places available) JCOP - Joint PVSS-JCOP Frameswork (course in English): 21.5.-25.5.2007 (5 days, 12 places available) JCOP - Finite State Machines in the JCOP Frameswork (course in English): 12.6.-14.6.2007 (3 days, 12 places available) LabVIEW Basics 1 (in English): 2.-4.5.2007 (3 days, 7 places ...

  6. Technical training - places available

    CERN Multimedia

    2012-01-01

    If you would like more information on a course, or for any other inquiry/suggestions, please contact Technical.Training@cern.ch Valeria Perez Reale, Learning Specialist, Technical Programme Coordinator (Tel.: 62424) Eva Stern and Elise Romero, Technical Training Administration (Tel.: 74924) HR Department »Electronics design Next Session Duration Language Availability Comprehensive VHDL for FPGA Design 08-Oct-12 to 12-Oct-12 5 days English 3 places available Foundations of Electromagnetism and Magnet Design (EMAG) 14-Nov-12 to 27-Nov-12 6 days English 20 places available Impacts de la suppression du plomb (RoHS) en électronique 26-Oct-12 to 26-Oct-12 8 hours French 15 places available Introduction to VHDL 10-Oct-12 to 11-Oct-12 2 days English 7 places available LabVIEW Real Time and FPGA 13-Nov-12 to 16-Nov-12 5 days French 5 places available »Mechanical design Next Se...

  7. Place-Specific Computing

    DEFF Research Database (Denmark)

    Messeter, Jörn; Johansson, Michael

    An increased interest in the notion of place has evolved in interaction design. Proliferation of wireless infrastructure, developments in digital media, and a ‘spatial turn’ in computing provides the base for place-specific computing as a suggested new genre of interaction design. In the REcult...... project place- specific computing is explored through design oriented research. This article reports six pilot studies where design students have designed concepts for place-specific computing in Berlin (Germany), Cape Town (South Africa), Rome (Italy) and Malmö (Sweden). Background and arguments...... for place-specific computing as a genre of interaction design are described. A total number of 36 design concepts designed for 16 designated zones in the four cities are presented. An analysis of the design concepts is presented indicating potentials, possibilities and problems as directions for future...

  8. Management and communication courses – Places available

    CERN Multimedia

    2013-01-01

    There are places available in some management and communication courses taking place in the period April to June 2013.   For more information on the course, click on the course title, this will bring you to the training catalogue. You can then sign-up online. For advice, you can contact: - Erwin Mosselmans, tel. 74125, erwin.mosselmans@cern.ch - Nathalie Dumeaux, tel. 78144, nathalie.dumeaux@cern.ch Courses in English (or bilingual) Sessions Duration Language Availability Managing stress 29 and 30 May 2 days English 3 places Making Presentations 30, 31 May & 25 June 3 days English 2 places Communicating Effectively - Residential course 4 to 6 June 3 days Bilingual 9 places Handling difficult conversations (Adapted from Dealing with Conflict) 7 and 14 June and 13 September 3 days English 6 places Voice and Nonverbal Behaviour in Speech Communication 17 and 18 June 1 day 4 hours English 7 plac...

  9. Connecting people to place : Sustainable place-shaping practices as transformative power.

    NARCIS (Netherlands)

    Horlings, Lummina

    2016-01-01

    The article describes a process of preparing a research design on place-shaping, as outcome of a process of co-design between academic actors and non-academic actors in Brazil, South Africa and The Netherlands, taking place in the context of the project TRANSPLACE. The joint research design

  10. Brand new authentic places

    DEFF Research Database (Denmark)

    Stender, Marie

    created and transformed by architects and designers, estate agents and, eventually, residents? Based on ethnographic fieldwork in 3-4 new residential neighbourhoods in the Copenhagen Region, the project will investigate how stories of authenticity give places meaning and identity. The project will unfold...... neighbourhoods stories of authenticity seems to be of great importance giving value and identity to place and people. By way of design and branding new places are implied with notions of the real, the original and the unique referring to e.g. its historical past, architectural uniqueness, sustainability or sense...

  11. Sporulation and diversity of arbuscular mycorrhizal fungi in Brazil Pine in the field and in the greenhouse.

    Science.gov (United States)

    Moreira, Milene; Nogueira, Marco A; Tsai, Siu M; Gomes-da-Costa, Sandra M; Cardoso, Elke J B N

    2007-09-01

    The aim of this work was to assess the sporulation and diversity of arbuscular mycorrhizal fungi (AMF) at different forest sites with Araucaria angustifolia (Bert.) O. Ktze. (Brazil Pine). In addition, a greenhouse experiment was carried out to test the use of traditional trap plants (maize + peanut) or A. angustifolia to estimate the diversity of AMF at each site. Soil samples were taken in two State Parks at southwestern Brazil: Campos do Jordão (Parque Estadual de Campos do Jordão [PECJ]) and Apiaí (Parque Estadual Turístico do Alto Ribeira [PETAR]), São Paulo State, in sites of either native or replanted forest. In PECJ, an extra site of replanted forest that was impacted by accidental fire and is now in a state of recuperation was also sampled. The spore densities and their morphological identification were compiled at each site. In the greenhouse, soil samples from each site were used as inoculum to promote spore multiplication on maize + peanut or A. angustifolia grown on a sandy, low-fertility substrate. Plants were harvested, respectively, after 4 months or 1 year of growth and assessed for mycorrhizal root colonization. Spore counts and identification were also performed in the substrate, after the harvest of plants. Twenty-five taxa were identified considering all sites. Species richness and diversity were greater in native forest areas, being Acaulospora, the genus with the most species. Differences in number of spores, diversity, and richness were found at the different sites of each State Park. Differences were also found when maize + peanut or A. angustifolia were used as trap plants. The traditional methodology using trap plants seems to underestimate the diversity of the AMF. The use of A. angustifolia as trap plant showed similar species richness to the field in PECJ, but the identified species were not necessarily the same. Nevertheless, for PETAR, both A. angustifolia and maize + peanut underestimated the species richness. Because the AMF

  12. Technical training - Places available

    CERN Multimedia

    2012-01-01

    If you would like more information on a course, or for any other inquiry/suggestions, please contact Technical.Training@cern.ch Valeria Perez Reale, Learning Specialist, Technical Programme Coordinator (Tel.: 62424) Eva Stern and Elise Romero, Technical Training Administration (Tel.: 74924)   Electronics design Next Session Duration Language Availability Certified LabVIEW Associate Developer (CLAD) 06-Dec-12 to 06-Dec-12 1 hour English One more place available Compatibilité électromagnetique (CEM): Applications 23-Nov-12 to 23-Nov-12 3.5 hours English 3 places available Compatibilité électromagnétique (CEM): Introduction 23-Nov-12 to 23-Nov-12 3 hours English 43 places available Effets des Radiations sur les composants et systèmes électroniques 11-Dec-12 to 12-Dec-12 1 day 4 hours French 9 places available LabVIEW for beginners ...

  13. History, Criticism and Place

    DEFF Research Database (Denmark)

    Hinds, Mat; Carter, Adrian; Malpas, Jeff

    2012-01-01

    Rory Spence and Richard Leplastrier shared a conversation and friendship that lasted 20years until Spence's death in 2004. The discussions focused largely upon issues of place, distilled through the practice of Leplastrier....

  14. Marine Place Names

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains the geographic place names for features in the U.S territorial waters and outer continental shelf. These names can be used to find or define a...

  15. Parks, People and Places

    DEFF Research Database (Denmark)

    Molin, Julie Frøik

    This thesis suggests, by applying a ‘place’ perspective, that involving users in operational management of green spaces holds potentials for enhancing ‘place attachment’ of urban inhabitants. As this can also build their commitment to decision making on their local living environment, green space...... maintenance becomes a potentially important setting for governance processes. Through a number of qualitative case studies, based on semi-structured interviews with municipal and community actors in Denmark and England, this dissertation explores the status and potentials of applying a place-based governance...... dimensions ‘actors’, ‘resources’ and ‘rules’. Moreover, the impacts of place-based governance processes on green space quality are examined via a review of previous scientific articles. The thesis concludes that place-based governance offers potential for bringing users and managers closer together, through...

  16. 'A Place to Sit'

    DEFF Research Database (Denmark)

    Hvejsel, Marie Frier; Klok, Julie Skovgaard; Bøhnke, Mia Marker

    Published in 2014 on the occasion of the third 'A Place to Sit' exhibition as a reflection upon three years of teaching tectonic method in architecture using the furniture scale as a learning basis.......Published in 2014 on the occasion of the third 'A Place to Sit' exhibition as a reflection upon three years of teaching tectonic method in architecture using the furniture scale as a learning basis....

  17. Self-Placing Concrete

    OpenAIRE

    ECT Team, Purdue

    2007-01-01

    Certain concrete pours have areas where the congestion of reinforcing bars make placement of concrete almost impossible. Using conventional placing and vibration techniques, the resulting concrete can have considerable honeycombing due to the development of voids. Self-placing concrete is a possible solution to the problem. Also known as self-compactable concrete, self-consolidating concrete, flowable concrete, and non-vibration concrete. These concretes eliminate the need for vibration in a ...

  18. Technical training - Places available

    CERN Multimedia

    2012-01-01

    If you would like more information on a course, or for any other inquiry/suggestions, please contact Technical.Training@cern.ch Valeria Perez Reale, Learning Specialist, Technical Programme Coordinator (Tel.: 62424) Eva Stern and Elise Romero, Technical Training Administration (Tel.: 74924) HR Department Electronic Design Next Session Duration Language Availability Comprehensive VHDL for FPGA Design 08-Oct-12 to 12-Oct-12 5 days English 4 places Electrostatique / Protection ESD 28-Sep-12 to 28-Sep-12 3 hours French 25 places Impacts de la suppression du plomb (RoHS) en électronique 26-Oct-12 to 26-Oct-12 8 hours French 14 places Introduction to VHDL 10-Oct-12 to 11-Oct-12 2 days English 9 places LabVIEW Real Time and FPGA 13-Nov-12 to 16-Nov-12 5 days French 5 places LabVIEW for Experts 24-Sep-12 to 28-Sep-12 5 days English 6 places LabVIEW for beginners 15-Oct-12 to 17-...

  19. Increase of the Bacillus thuringiensis secreted toxicity against lepidopteron larvae by homologous expression of the vip3LB gene during sporulation stage

    OpenAIRE

    Sellami, Sameh; Jamoussi, Kais; Dabbeche, Emna; Jaoua, Samir

    2011-01-01

    The Vegetative insecticidal Vip3A proteins display a wide range of insecticidal spectrum against several agricultural insect pests. The fact that the expression of vip3 genes occurs only during the vegetative growth phase of Bacillus thuringiensis is a limiting factor in term of production level. Therefore, extending the synthesis of the Vip proteins to the sporulation phase is a good alternative to reach high levels of toxin synthesis. In this study, we have demonstrated that the maximal pro...

  20. Quantitative proteome analysis of Streptomyces coelicolor Nonsporulating liquid cultures demonstrates a complex differentiation process comparable to that occurring in sporulating solid cultures

    DEFF Research Database (Denmark)

    Manteca, Angel; Jung, Hye R; Schwämmle, Veit

    2010-01-01

    involved in primary metabolism (ribosome, Krebs cycle, and energy production) were detected in greater abundance in MI. The most remarkable protein abundance differences between MII from solid and liquid cultures were associated with the final stages of hyphae compartmentalization and spore formation.......Streptomyces species produce many clinically important secondary metabolites and present a complex developmental cycle that includes programmed cell death (PCD) phenomena and sporulation. Industrial fermentations are usually performed in liquid cultures, conditions in which Streptomyces strains...

  1. Taking technological infrastructure seriously

    NARCIS (Netherlands)

    Mair, C.S.

    2017-01-01

    Taking Technological Infrastructure Seriously attempts to take stock of a sea-change in the way modern infrastructural resources are provided. Unlike traditional infrastructure, such as roads and electricity cables, where the State has largely been responsible for its provision, much of the key

  2. Mathematics of Risk Taking

    Indian Academy of Sciences (India)

    user

    Mathematics of Risk Taking. K B Athreya and M G Nadkarni. Keywords. Investor, risk taking, fair game, martingale, random walk, gam- bling, population. (left) K B Athreya is a visiting professor at IMI,. Mathematics Department,. IISc, Bangalore and is also a professor of mathematics and statistics at Iowa State. University ...

  3. Growth and sporulation defects in Bacillus subtilis mutants with a single rrn operon can be suppressed by amplification of the rrn operon.

    Science.gov (United States)

    Yano, Koichi; Masuda, Kenta; Akanuma, Genki; Wada, Tetsuya; Matsumoto, Takashi; Shiwa, Yuh; Ishige, Taichiro; Yoshikawa, Hirofumi; Niki, Hironori; Inaoka, Takashi; Kawamura, Fujio

    2016-01-01

    The genome of Bacillus subtilis strain 168 encodes ten rRNA (rrn) operons. We previously reported that strains with only a single rrn operon had a decreased growth and sporulation frequency. We report here the isolation and characterization of suppressor mutants from seven strains that each have a single rrn operon (rrnO, A, J, I, E, D or B). The suppressor mutants for strain RIK656 with a single rrnO operon had a higher frequency of larger colonies. These suppressor mutants had not only increased growth rates, but also increased sporulation frequencies and ribosome levels compared to the parental mutant strain RIK656. Quantitative PCR analyses showed that all these suppressor mutants had an increased number of copies of the rrnO operon. Suppressor mutants were also isolated from the six other strains with single rrn operons (rrnA, J, I, E, D or B). Next generation and capillary sequencing showed that all of the suppressor mutants had tandem repeats of the chromosomal locus containing the remaining rrn operon (amplicon). These amplicons varied in size from approximately 9 to 179 kb. The amplifications were likely to be initiated by illegitimate recombination between non- or micro-homologous sequences, followed by unequal crossing-over during DNA replication. These results are consistent with our previous report that rrn operon copy number has a major role in cellular processes such as cell growth and sporulation.

  4. The cisA cistron of Bacillus subtilis sporulation gene spoIVC encodes a protein homologous to a site-specific recombinase.

    Science.gov (United States)

    Sato, T; Samori, Y; Kobayashi, Y

    1990-02-01

    The nucleotide sequence of the sporulation gene spoIVC cisA in Bacillus subtilis was determined and found to encode a protein of 500 amino acid residues with a calculated molecular weight of 57,481, which is in good agreement with the size of the gene product estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The amino acid sequence of the N-terminal region of this protein is homologous to the site-specific DNA recombinases. Hybridization of a 3.6-kilobase EcoRI fragment carrying the spoIVC cisA gene with the EcoRI-restricted chromosomal DNA prepared from cells of various stages showed that DNA rearrangement occurs only in the mother cell in the region adjacent to spoIVC cisA 3 h after the initiation of sporulation. This result coincides with that of Stragier et al. (P. Stragier, B. Kunkel, L. Kroos, and R. Losick, Science 243:507-512, 1989). The timing of the DNA rearrangement coincides very well with the timing of spoIVC cisA gene expression. The DNA rearrangement was not observed in spoIVC cisA mutants. These results strongly suggest that the spoIVC cisA gene encodes a site-specific DNA recombinase having a very important role in sporulation.

  5. In vitro cytotoxicity induced by Clostridium perfringens isolate carrying a chromosomal cpe gene is exclusively dependent on sporulation and enterotoxin production.

    Science.gov (United States)

    Yasugi, Mayo; Sugahara, Yuki; Hoshi, Hidenobu; Kondo, Kaori; Talukdar, Prabhat K; Sarker, Mahfuzur R; Yamamoto, Shigeki; Kamata, Yoichi; Miyake, Masami

    2015-08-01

    Clostridium perfringens type A is a common source of food poisoning (FP) and non-food-borne (NFB) gastrointestinal diseases in humans. In the intestinal tract, the vegetative cells sporulate and produce a major pathogenic factor, C. perfringens enterotoxin (CPE). Most type A FP isolates carry a chromosomal cpe gene, whereas NFB type A isolates typically carry a plasmid-encoded cpe. In vitro, the purified CPE protein binds to a receptor and forms pores, exerting a cytotoxic activity in epithelial cells. However, it remains unclear if CPE is indispensable for C. perfringens cytotoxicity. In this study, we examined the cytotoxicity of cpe-harboring C. perfringens isolates co-cultured with human intestinal epithelial Caco-2 cells. The FP strains showed severe cytotoxicity during sporulation and CPE production, but not during vegetative cell growth. While Caco-2 cells were intact during co-culturing with cpe-null mutant derivative of strain SM101 (a FP strain carrying a chromosomal cpe gene), the wild-type level cytotoxicity was observed with cpe-complemented strain. In contrast, both wild-type and cpe-null mutant derivative of the NFB strain F4969 induced Caco-2 cell death during both vegetative and sporulation growth. Collectively, the Caco-2 cell cytotoxicity caused by C. perfringens strain SM101 is considered to be exclusively dependent on CPE production, whereas some additional toxins should be involved in F4969-mediated in vitro cytotoxicity. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Hexameric assembly of membrane fusion protein YknX of the sporulation delaying efflux pump from Bacillus amyloliquefaciens.

    Science.gov (United States)

    Xu, Yongbin; Jo, Inseong; Wang, Lulu; Chen, Jinli; Fan, Shengdi; Dong, Yuesheng; Quan, Chunshan; Ha, Nam-Chul

    2017-11-04

    Membrane fusion proteins (MFPs) play an essential role in the action of the drug efflux pumps and protein secretion systems in bacteria. The sporulation delaying protein (SDP) efflux pump YknWXYZ has been identified in diverse Bacillus species. The MFP YknX requires the ATP-binding cassette (ABC) transporter YknYZ and the Yip1 family protein YknW to form a functional complex. To date, the crystal structure, molecular function and mechanism of action of YknX remain unknown. In this study, to characterize the structural and biochemical roles of YknX in the functional assembly of YknWXYZ from B. amyloliquefaciens, we successfully obtained crystals of the YknX protein that diffracted X-rays to a resolution of 4.4 Å. We calculated an experimentally phased map using single-wavelength anomalous diffraction (SAD), revealing that YknX forms a hexameric assembly similar to that of MacA from Gram-negative bacteria. The hexameric assembly of YknX exhibited a funnel-like structure with a central channel and a conical mouth. Functional studies in vitro suggest that YknX can bind directly to peptidoglycan. Our study provides an improved understanding of the assembly of the YknWXYZ efflux pump and the role of YknX in the complex. Copyright © 2017 Elsevier Inc. All rights reserved.

  7. A versatile class of cell surface directional motors gives rise to gliding motility and sporulation in Myxococcus xanthus.

    Directory of Open Access Journals (Sweden)

    Morgane Wartel

    2013-12-01

    Full Text Available Eukaryotic cells utilize an arsenal of processive transport systems to deliver macromolecules to specific subcellular sites. In prokaryotes, such transport mechanisms have only been shown to mediate gliding motility, a form of microbial surface translocation. Here, we show that the motility function of the Myxococcus xanthus Agl-Glt machinery results from the recent specialization of a versatile class of bacterial transporters. Specifically, we demonstrate that the Agl motility motor is modular and dissociates from the rest of the gliding machinery (the Glt complex to bind the newly expressed Nfs complex, a close Glt paralogue, during sporulation. Following this association, the Agl system transports Nfs proteins directionally around the spore surface. Since the main spore coat polymer is secreted at discrete sites around the spore surface, its transport by Agl-Nfs ensures its distribution around the spore. Thus, the Agl-Glt/Nfs machineries may constitute a novel class of directional bacterial surface transporters that can be diversified to specific tasks depending on the cognate cargo and machinery-specific accessories.

  8. Sporulation capability and amylosome conservation among diverse human colonic and rumen isolates of the keystone starch‐degrader Ruminococcus bromii

    Science.gov (United States)

    Mukhopadhya, Indrani; Moraïs, Sarah; Laverde‐Gomez, Jenny; Sheridan, Paul O.; Walker, Alan W.; Kelly, William; Klieve, Athol V.; Ouwerkerk, Diane; Duncan, Sylvia H.; Louis, Petra; Koropatkin, Nicole; Cockburn, Darrell; Kibler, Ryan; Cooper, Philip J.; Sandoval, Carlos; Crost, Emmanuelle; Juge, Nathalie; Bayer, Edward A.

    2017-01-01

    Summary Ruminococcus bromii is a dominant member of the human colonic microbiota that plays a ‘keystone’ role in degrading dietary resistant starch. Recent evidence from one strain has uncovered a unique cell surface ‘amylosome’ complex that organizes starch‐degrading enzymes. New genome analysis presented here reveals further features of this complex and shows remarkable conservation of amylosome components between human colonic strains from three different continents and a R. bromii strain from the rumen of Australian cattle. These R. bromii strains encode a narrow spectrum of carbohydrate active enzymes (CAZymes) that reflect extreme specialization in starch utilization. Starch hydrolysis products are taken up mainly as oligosaccharides, with only one strain able to grow on glucose. The human strains, but not the rumen strain, also possess transporters that allow growth on galactose and fructose. R. bromii strains possess a full complement of sporulation and spore germination genes and we demonstrate the ability to form spores that survive exposure to air. Spore formation is likely to be a critical factor in the ecology of this nutritionally highly specialized bacterium, which was previously regarded as ‘non‐sporing’, helping to explain its widespread occurrence in the gut microbiota through the ability to transmit between hosts. PMID:29159997

  9. Place Reference In Interaction

    Directory of Open Access Journals (Sweden)

    Enfield N.J.

    2017-12-01

    Full Text Available The language of place and space has been intensively studied in relation to grammatical characteristics, cross-linguistic variation, and cognition, as well as with regard to further questions central to social anthropology, psychology, and more. With this special issue, we focus on the pragmatic functions of references to places, as observed in informal social interaction. When people make reference to places in casual, everyday conversation, how do they do it, in what situations, and to what ends? We offer the first collection of findings from research on place reference in spontaneous, multi-party speech, with studies based on conversations recorded in the diverse geographic and cultural environments of outback Australia, highland New Guinea, island Indonesia and rural Mexico. The authors explore, from a range of angles, how and why people talk about place, for example, in regard to the vocabulary and grammar that a language has available to categorise space, and how people choose from among referential options in situated conversation to achieve communicative, social, and practical goals.

  10. Planned place of birth

    DEFF Research Database (Denmark)

    Overgaard, Charlotte; Coxon, Kirstie; Stewart, Mary

    in Denmark Coxon K et al: Planned place of birth in England: perceptions of accessing obstetric units, midwife led units and home birth amongst women and their partners. How these papers interrelate These papers draw upon recent research in maternity care, undertaken in Denmark and in England. In both......Title Planned place of birth: issues of choice, access and equity. Outline In Northern European countries, giving birth is generally safe for healthy women with uncomplicated pregnancies, and their babies. However, place of birth can affect women’s outcomes and experiences of birth. Whilst tertiary...... centres provide appropriate medical supervision to women with complex pregnancies, the likelihood of receiving interventions including surgical birth is increased for low risk women in these settings. In this symposium, we consider issues of choice, access and equitable care for women in the context...

  11. (Re)tasting places

    DEFF Research Database (Denmark)

    Hedegaard, Liselotte

    2015-01-01

    What does geographical origin mean? It is an expression that associates food and wine with a specific place, an association embedded in the concept ‘terroir’ that refers to the complex interaction between a physical environment and local craftsmanship. It is a claim protected through labelling......-schemes and a claim that adds value to the place-related foods. However, viewing the connection between food and place as a question of proving a relationship or as a matter of protecting commercial claims does not seem to provide a satisfactory account for the status of geographically designated foods as being...... particularly attractive Central to the interest of this paper is to approach an understanding of geographical origin as a point of reference for taste. In terms of being sensory experience, taste is subjective. It is difficult to describe verbally and yet at the same time it is a trigger of the memory of past...

  12. 1. On note taking.

    Science.gov (United States)

    Plaut, Alfred B J

    2005-02-01

    In this paper the author explores the theoretical and technical issues relating to taking notes of analytic sessions, using an introspective approach. The paper discusses the lack of a consistent approach to note taking amongst analysts and sets out to demonstrate that systematic note taking can be helpful to the analyst. The author describes his discovery that an initial phase where as much data was recorded as possible did not prove to be reliably helpful in clinical work and initially actively interfered with recall in subsequent sessions. The impact of the nature of the analytic session itself and the focus of the analyst's interest on recall is discussed. The author then describes how he modified his note taking technique to classify information from sessions into four categories which enabled the analyst to select which information to record in notes. The characteristics of memory and its constructive nature are discussed in relation to the problems that arise in making accurate notes of analytic sessions.

  13. Ageing in Communal Place

    DEFF Research Database (Denmark)

    Aarhus, Rikke; Ballegaard, Stinne Aaløkke; Grönvall, Erik

    2009-01-01

    In this paper we adopt the position that design of social media for the elderly and virtual senior communities may be informed by studying ‘real’ senior communities. Since current research efforts target the role of social media and virtual communities for supporting seniors ageing in place, i.e....

  14. Ageing in communal place

    DEFF Research Database (Denmark)

    Aarhus, Rikke; Ballegaard, Stinne Aaløkke; Grönvall, Erik

    2009-01-01

    In this paper we adopt the position that design of social media for the elderly and virtual senior communities may be informed by studying `real´senior communities. Since current research efforts target the role of social media and virtual communities for supporting seniors ageing in place, i.e. ...

  15. The power of place.

    Science.gov (United States)

    Lock, Lin R; Gibb, Heather J

    2003-06-01

    to describe the power that 'place' holds over the postnatal-care experiences of women. a study informed by phenomenology within a feminist framework was undertaken to examine the experiences of women electing early postnatal discharge. Three extended conversations with each woman participating in the study were audiotaped and transcribed. Journal notes made by the researcher added to the audiotaped data. Thematic analysis revealed major structures of experience. data were obtained from conversations with women in their respective homes. five women, parity 1-3, living in the Sydney metropolitan area and birthing in their local hospitals participated in the study. four major constructs of experience were revealed through analysis and include spatiality, corporeality, temporality and relationality. In this paper, components of spatiality expressed through the power place exerts in matters of physical environment,control, confidence, safety, time, talk and the heart of the matter are presented. the experiences of women entering the foreign place of hospital to birth their children were those of alienation and disempowerment while the familiar territory of home offered stronger feelings of security and support. failing to recognise the impact of place on the experiences of postnatal women reduces the likelihood that midwives will be able to offer sensitive and appropriate care.

  16. Designing Places for Learning.

    Science.gov (United States)

    Meek, Anne, Ed.

    This book presents information about the condition of schools around the United States. It also describes the link between architecture and academic success and offers suggestions for improving the design of existing and future school buildings. Eleven articles look at schools as places of deep meaning and show how that view can alter approaches…

  17. Technical training - Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 16.5.2006 (May-November course sessions) Technical Training: Places available The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Title Hours Date Language ACROBAT 7.0 : Utilisation de fichiers PDF 8 8.05.06 F WORD 2003 - niveau 2 : ECDL 16 22-23.05.06 23-24.05.06 F Comprehensive VHDL for FPGA Design 40 29.05-2.06.06 E C++ Programming Part 2 - Advanced C++ and its Traps and Pitfalls 32 30.05-2.06.06 E ACROBAT 7.0 : Utilisation de fichiers PDF 24 7-9.06.06 E AutoCAD Mechanical 2006 16 13-14.06.06 F CERN EDMS for Local Administrators 16 13-14.06.06 E LabVIEW Base 2 32 27.06-5.07.06 F C++ Programming Part 3 - Templates and the STL (Standard Template Library) 16 27-28.06.06 E C++ Programming Part 4 - Exceptions 8 29.06.06 E FrontPage 2003 - niveau 1 16 29-...

  18. Teaching With Historic Places.

    Science.gov (United States)

    Greenberg, Ronald M., Ed.

    1993-01-01

    Designed for social studies educators, this theme issue presents 11 articles about historic places that feature a variety of ideas for elementary and secondary lesson plans, curricula, and program development. The articles are: (1) "Where did History Happen?" (Beth M. Boland); (2) "Creating a Partnership" (Carol D. Shull); (3)…

  19. Place in Transition

    DEFF Research Database (Denmark)

    Mikkelsen, Jacob Bjerre; Lange, Ida Sofie Gøtzsche

    from the 'Everyday World'. Within mobilities studies, research has focused on different aspects and consequences of the post-oil society (see Dennis & Urry 2009, Urry 2013). This paper discusses the conception of place within the enclosed 'Oil World' with point of departure in relocation...

  20. Technical training - Places available

    CERN Multimedia

    2003-01-01

    * Etant donné le délai d'impression du Bulletin, ces places peuvent ne plus être disponibles au moment de sa parution. Veuillez consulter notre site Web pour avoir la dernière mise à jour. ** The number of places available may vary. Please check our Web site to find out the current availability. Des places sont disponibles dans les cours suivants : Places are available in the following courses: Hands-on Introduction to Python Programming 12 – 14.11.03 (3 days) ACCESS 2000 – niveau 1 13 & 14.11.03 (2 jours) C++ for Particle Physicists 17 – 21.11.03 (6 x 3-hour lectures) Programmation automate Schneider TSX Premium – niveau 2  18 – 21.11.03 (4 jours) Planification de projet avec MS-Project/Project Planning with MS-Project (gratuit/free of charge – langue à définir/language to be defined) : 18 & 25.11.03 (2 jours/2 days) JAVA 2 Enterprise Edition – Part 1 : WEB...

  1. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The Joint PVSS JCOP Framework: 14 - 18.6.2004 (5 days) EXCEL 2003 - niveau 2 : 17 & 18.6.2004 (2 jours) MAGNE-04 : Magnétisme pour l'électrotechnique : 6 au 8.7.2004 (3 jours) Technical Training Monique Duval - Tel.74924 technical.training@cern.ch

  2. Taking a Long and Global View

    DEFF Research Database (Denmark)

    Ougaard, Morten

    ‘the free development of each is the condition for the free development of all’. And the fourth element is the idea that the transition to this new and better society will take place through a revolution led by the industrial working class. These elements combine outstanding and path-breaking social...

  3. Teachable Moment: Google Earth Takes Us There

    Science.gov (United States)

    Williams, Ann; Davinroy, Thomas C.

    2015-01-01

    In the current educational climate, where clearly articulated learning objectives are required, it is clear that the spontaneous teachable moment still has its place. Authors Ann Williams and Thomas Davinroy think that instructors from almost any discipline can employ Google Earth as a tool to take advantage of teachable moments through the…

  4. The Goodwin model: simulating the effect of light pulses on the circadian sporulation rhythm of Neurospora crassa.

    Science.gov (United States)

    Ruoff, P; Vinsjevik, M; Monnerjahn, C; Rensing, L

    2001-03-07

    The Goodwin oscillator is a minimal model that describes the oscillatory negative feedback regulation of a translated protein which inhibits its own transcription. Now, over 30 years later this scheme provides a basic description of the central components in the circadian oscillators of Neurospora, Drosophila, and mammals. We showed previously that Neurospora's resetting behavior by pulses of temperature, cycloheximide or heat shock can be simulated by this model, in which degradation processes play an important role for determining the clock's period and its temperature-compensation. Another important environmental factor for the synchronization is light. In this work, we show that on the basis of a light-induced transcription of the frequency (frq) gene phase response curves of light pulses as well as the influence of the light pulse length on phase shifts can be described by the Goodwin oscillator. A relaxation variant of the model predicts that directly after a light pulse inhibition in frq -transcription occurs, even when the inhibiting factor Z (FRQ) has not reached inhibitory concentrations. This has so far not been experimentally investigated for frq transcription, but it complies with a current model of light-induced transcription of other genes by a phosphorylated white-collar complex. During long light pulses, the relaxational model predicts that the sporulation rhythm is arrested in a steady state of high frq -mRNA levels. However, experimental results indicate the possibility of oscillations around this steady state and more in favor of the results by the original Goodwin model. In order to explain the resetting behavior by two light pulses, a biphasic first-order kinetics recovery period of the blue light receptor or of the light signal transduction pathway has to be assumed. Copyright 2001 Academic Press.

  5. Heat shock and prolonged heat stress attenuate neurotoxin and sporulation gene expression in group I Clostridium botulinum strain ATCC 3502.

    Science.gov (United States)

    Selby, Katja; Mascher, Gerald; Somervuo, Panu; Lindström, Miia; Korkeala, Hannu

    2017-01-01

    Foodborne pathogenic bacteria are exposed to a number of environmental stresses during food processing, storage, and preparation, and in the human body. In order to improve the safety of food, the understanding of molecular stress response mechanisms foodborne pathogens employ is essential. Many response mechanisms that are activated during heat shock may cross-protect bacteria against other environmental stresses. To better understand the molecular mechanisms Clostridium botulinum, the causative agent of botulism, utilizes during acute heat stress and during adaptation to stressfully high temperature, the C. botulinum Group I strain ATCC 3502 was grown in continuous culture at 39°C and exposed to heat shock at 45°C, followed by prolonged heat stress at 45°C to allow adaptation of the culture to the high temperature. Growth in continuous culture was performed to exclude secondary growth phase effects or other environmental impacts on bacterial gene transcription. Changes in global gene expression profiles were studied using DNA microarray hybridization. During acute heat stress, Class I and III heat shock genes as well as members of the SOS regulon were activated. The neurotoxin gene botA and genes encoding the neurotoxin-associated proteins were suppressed throughout the study. Prolonged heat stress led to suppression of the sporulation machinery whereas genes related to chemotaxis and motility were activated. Induced expression of a large proportion of prophage genes was detected, suggesting an important role of acquired genes in the stress resistance of C. botulinum. Finally, changes in the expression of a large number of genes related to carbohydrate and amino acid metabolism indicated remodeling of the cellular metabolism.

  6. Compost Addition Enhanced Hyphal Growth and Sporulation of Arbuscular Mycorrhizal Fungi without Affecting Their Community Composition in the Soil

    Science.gov (United States)

    Yang, Wei; Gu, Siyu; Xin, Ying; Bello, Ayodeji; Sun, Wenpeng; Xu, Xiuhong

    2018-01-01

    Arbuscular mycorrhizal (AM) fungi form symbiotic associations with most crop plant species in agricultural ecosystems, and are conspicuously influenced by various agricultural practices. To understand the impact of compost addition on AM fungi, we examined effect of four compost rates (0, 11.25, 22.5, and 45 Mg/ha) on the abundance and community composition of AM fungi in seedling, flowering, and mature stage of soybean in a 1-year compost addition experiment system in Northeast China. Soybean [Glycine max (L.) Merrill] was used as test plant. Moderate (22.5 Mg/ha) and high (45 Mg/ha) levels of compost addition significantly increased AM root colonization and extraradical hyphal (ERH) density compared with control, whereas low (11.5 Mg/ha) level of compost addition did not cause significant increase in AM root colonization and ERH density. AM fungal spore density was significantly enhanced by all the compost rates compared with control. The temporal variations analysis revealed that, AM root colonization in seedling stage was significantly lower than in flowering and mature stage. Although AM fungal operational taxonomic unit richness and community composition was unaffected by compost addition, some abundant AM fungal species showed significantly different response to compost addition. In mature stage, Rhizophagus fasciculatum showed increasing trend along with compost addition gradient, whereas the opposite was observed with Paraglomus sp. In addition, AM fungal community composition exhibited significant temporal variation during growing season. Further analysis indicated that the temporal variation in AM fungal community only occurred in control treatment, but not in low, moderate, and high level of compost addition treatments. Our findings highlighted the significant effects of compost addition on AM growth and sporulation, and emphasized that growth stage is a stronger determinant than 1-year compost addition in shaping AM fungal community in black soil of

  7. Compost Addition Enhanced Hyphal Growth and Sporulation of Arbuscular Mycorrhizal Fungi without Affecting Their Community Composition in the Soil

    Directory of Open Access Journals (Sweden)

    Wei Yang

    2018-02-01

    Full Text Available Arbuscular mycorrhizal (AM fungi form symbiotic associations with most crop plant species in agricultural ecosystems, and are conspicuously influenced by various agricultural practices. To understand the impact of compost addition on AM fungi, we examined effect of four compost rates (0, 11.25, 22.5, and 45 Mg/ha on the abundance and community composition of AM fungi in seedling, flowering, and mature stage of soybean in a 1-year compost addition experiment system in Northeast China. Soybean [Glycine max (L. Merrill] was used as test plant. Moderate (22.5 Mg/ha and high (45 Mg/ha levels of compost addition significantly increased AM root colonization and extraradical hyphal (ERH density compared with control, whereas low (11.5 Mg/ha level of compost addition did not cause significant increase in AM root colonization and ERH density. AM fungal spore density was significantly enhanced by all the compost rates compared with control. The temporal variations analysis revealed that, AM root colonization in seedling stage was significantly lower than in flowering and mature stage. Although AM fungal operational taxonomic unit richness and community composition was unaffected by compost addition, some abundant AM fungal species showed significantly different response to compost addition. In mature stage, Rhizophagus fasciculatum showed increasing trend along with compost addition gradient, whereas the opposite was observed with Paraglomus sp. In addition, AM fungal community composition exhibited significant temporal variation during growing season. Further analysis indicated that the temporal variation in AM fungal community only occurred in control treatment, but not in low, moderate, and high level of compost addition treatments. Our findings highlighted the significant effects of compost addition on AM growth and sporulation, and emphasized that growth stage is a stronger determinant than 1-year compost addition in shaping AM fungal community in

  8. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 9.5.2006 (May-October course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: find out the curren Hours Date Language LabVIEW Application Development 24 15-17.05.06 E LabVIEW Advanced Programming 16 18-19.05.06 E PERL 5: Advanced Aspects 8 18.05.06 E Technique du vide 16 18-19.05.06 F FileMaker - niveau 2 16 11-12.05.06 F WORD 2003 - niveau 2 : ECDL 16 22-23.05.06 F Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE 16 23-24.05.06 F Comprehensive VHDL for FPGA Design 40 29.05-2.06.06 E C++ Programming Part 2 - Advanced C++ and its Traps and Pitfalls 32 30.05-2.06.06 E Python: Hands-on Introduction 24 7-9.06.06 E AutoCAD Mechanical 2006 16 13-14.06.06 F CERN EDMS for Local Administrators 16 13-14.06.06...

  9. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 7.2.2006 (February-May course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Title Hours Date Language WORD 2003 (Short Course IV) - HowTo... Work with master document 3 27.02.06 E-F JAVA: Level 2 32 28.02-3.03.06 E Manipulation des images 4 28.02.06 F ACCESS 2003 - Level 2: ECDL AM5 16 2-3.03.06 E-F C++ for Particle Physicists 20 6-10.03.06 E PowerPoint 2003 8 9.03.06 F JCOP: Control System Integration using JCOP Tools 24 14-16.03.06 E EXCEL 2003 (Short Course III) - HowTo... Pivot tables 3 20.03.06 E-F EXCEL 2003 (Short Course IV) - HowTo....Link cells, worksheets and workbooks 3 20.03.06 E-F JCOP: Finite State Machines in the JCOP Framework 24 21-23.03.06 E Object-Oriented Analysis and Design using UML 24 21-23.03.06 E FrontPage 2003 - niveau 1 16 27-28.03.06 F JCOP: Joint PVSS-JCOP Fram...

  10. Technical Training: Places available

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    Davide Vitè

    2006-01-01

    Places available as of 7.2.2006 (February-May course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Title Hours Date Language WORD 2003 (Short Course II) - HowTo... Mail merge 3 13.03.06 E-F EXCEL 2003 (Short Course III) - HowTo... Pivot tables 3 20.03.06 E-F EXCEL 2003 (Short Course IV) - HowTo....Link cells, worksheets and workbooks 3 20.03.06 E-F Object-Oriented Analysis and Design using UML 24 21-23.03.06 E EXCEL 2003 - niveau 1 16 22-23.03.06 F FrontPage 2003 - niveau 1 16 27-28.03.06 F Oracle Forms Developer 10g: Move to the Web 16 27-28.03.06 E Oracle JDeveloper 10g: Build Applications with ADF 24 29-31.03.06 E ACCESS 2003 - Level 2: ECDL AM5 16 3-4.03.06 E-F JAVA 2 Enterprise Edition - Part 1: Web Applications 16 3-4.04.06 E JCOP: Control System Integration using JCOP Tools 24 4-6.04.06 E JAVA 2 Enterprise Edition...

  11. Technical Training: Places available

    CERN Multimedia

    2006-01-01

    Places available as of 21.3.2006 (March-October course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: find out the curren Hours Date Language ACROBAT 7.0 : Utilisation de fichiers PDF 8 8.05.06 F Project Planning with MS-Project 16 9.05-6.06.06 E STEP7: niveau 1 32 9-12.05.06 E-F Oracle: Programming with PL/SQL 24 10-12.05.06 E FileMaker - niveau 2 16 11-12.05.06 F LabVIEW Application Development 24 15-17.05.06 E LabVIEW Advanced Programming 16 18-19.05.06 E PERL 5: Advanced Aspects 8 18.05.06 E Technique du vide 16 18-19.05.06 F WORD 2003 - niveau 2 : ECDL 16 22-23.05.06 F Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE 16 23-24.05.06 F Comprehensive VHDL for FPGA Design 40 29.05-2.06.06 E C++ Programming Part 2 - Advanced C++ and its T...

  12. Technical Training: Places available

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    Davide Vitè

    2006-01-01

    Places available as of 30.5.2006 (June-November course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Titre Heure Date Langue ACROBAT 7.0 : Utilisation de fichiers PDF 8 6.06.06 F Introduction à InDesign 16 7-8.06.06 F Python: Hands-on Introduction 24 7-9.06.06 E LabVIEW Base 2 16 22-23.06.06 F FileMaker - niveau 1 16 26-27.06.06 F C++ Programming Part 3 - Templates and the STL (Standard Template Library) 16 27-28.06.06 E C++ Programming Part 4 - Exceptions 8 29.06.06 E FrontPage 2003 - niveau 1 16 29-30.06.06 F Manipulation des images 4 6.07.06 F Introduction to Databases and Database Design 16 11-12.07.06 E ACCESS 2003 - Level 1: ECDL 16 13-14.07.06 E-F Design Patterns 16 25-26.07.06 E Introduction à Dreamweaver MX 16 ...

  13. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 21.3.2006 (March-October course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Title Hours Date Language FrontPage 2003 - niveau 1 16 27-28.03.06 F Oracle Forms Developer 10g: Move to the Web 16 27-28.03.06 E ACCESS 2003 - Level 2: ECDL AM5 16 3-4.03.06 E-F JAVA 2 Enterprise Edition - Part 1: Web Applications 16 3-4.04.06 E JAVA 2 Enterprise Edition - Part 2: Enterprise JavaBeans 24 5-7.04.06 E AutoCAD Mechanical 2006 16 11-12.04.06 F FrontPage 2003 - niveau 2 16 24-25.04.06 F C++ Programming Part 1 - Introduction to Object-Oriented Design and Programming 24 25-27.04.06 E AutoCAD 2006 - niveau 1 32 27.04-4.05.06 F Oracle: SQL 24 3-5.05.06 E EXCEL 2003 (Short Course I) - HowTo... Work with formulae 3 4.05.06 (am) E-F EXCEL 2003 (Short Course II) - HowTo... Format your worksheet for printing 3 4...

  14. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 7.2.2006 (February-May course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Title Hours Date Language WORD 2003 (Short Course II) - HowTo... Mail merge 3 09-02-06 E-F ACCESS 2003 - Level 1: ECDL M5 16 13 to 14-02-06 E-F OUTLOOK 2003 (Short Course II) - Calendar, Tasks and Notes 3 16-02-06 E-F WORD 2003 (Short Course III) - HowTo... Work with long documents 3 16-02-06 E-F CERN EDMS - Introduction 8 21.02.06 E OUTLOOK 2003 (Short Course III) - Meetings and Delegation 3 27-02-06 E-F WORD 2003 (Short Course IV) - HowTo... Work with master document 3 27-02-06 E-F JAVA: Level 2 32 28-02-06 to 03-03-06 E Manipulation des images 4 28.02.06 F ACCESS 2003 - Level 2: ECDL AM5 16 02 to 03-03-06 E-F FrontPage 2003 - niveau 2 16 02 to 03-03-06 F C++ for Particle Physicists 20 06 to 10-03-06 E FileMaker - niv...

  15. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 11.7.2006 (July-December course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Titre Heure Date Langue Design Patterns 16 25-26.07.06 E CERN EDMS for Local Administrators 16 1-2.08.06 E ANSYS DesignModeler 16 29-30.08.06 F CERN EDMS – Introduction 8 5.09.06 E CERN EDMS MTF en pratique 4 6.09.06 F LabVIEW Basics 1 24 4-6.09.06 E ANSYS Workbench 32 12-15.09.06 F AutoCAD Mechanical 2006 16 12-13.09.06 F CERN EDMS for Engineers 8 12.09.06 E Software Engineering in the Small and the Large 16 12-13.09.06 E LabVIEW Basics 2 16 14-15.09.06 E LabVIEW: Working efficiently with LabWIEW 8 8 18.09.06 E PCAD Schémas ? Introduction 16 21-22.09.06 F PCAD PCB - Introduction  24 27-29.09.06 F C++ for Particle Physicists ...

  16. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 27.6.2006 (July-December course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Titre Heure Date Langue Manipulation des images 4 6.07.06 F Introduction to Databases and Database Design 16 11-12.07.06 E ACCESS 2003 - Level 1: ECDL 16 13-14.07.06 E-F Design Patterns 16 25-26.07.06 E CERN EDMS for Local Administrators 16 1-2.08.06 E ANSYS DesignModeler 16 29-30.08.06 F CERN EDMS - Introduction 8 5.09.06 E CERN EDMS MTF en pratique 4 6.09.06 F LabVIEW Basics 1 24 4-6.09.06 E ANSYS Workbench 32 12-15.09.06 F AutoCAD Mechanical 2006 16 12-13.09.06 F CERN EDMS for Engineers 8 12.09.06 E Software Engineering in the Small and the Large 16 12-13.09.06 E AutoCAD 2006 - niveau 1 32 14-21.09.06 F LabVIEW Basics 2 ...

  17. Technical training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 13.6.2006 (June-December course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Titre Heure Date Langue LabVIEW Base 2 16 22-23.06.06 F FileMaker - niveau 1 16 26-27.06.06 F C++ Programming Part 3 - Templates and the STL (Standard Template Library) 16 27-28.06.06 E C++ Programming Part 4 - Exceptions 8 29.06.06 E FrontPage 2003 - niveau 1 16 29-30.06.06 F Manipulation des images 4 6.07.06 F Introduction to Databases and Database Design 16 11-12.07.06 E ACCESS 2003 - Level 1: ECDL 16 13-14.07.06 E-F Design Patterns 16 25-26.07.06 E Introduction à Dreamweaver MX 16 26-27.07.06 F ANSYS DesignModeler 16 29-30.08.06 F LabVIEW Basics 1 24 4-6.09.06 E ANSYS Workbench 32 12-15.09.06 F AutoCAD Mechanical 20...

  18. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 19.7.2006 (August-December course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Titre Heure Date Langue CERN EDMS for Local Administrators 16 1-2.08.06 E ANSYS DesignModeler 16 29-30.08.06 F OUTLOOK 2003 (Short Course I) - E-mail 3 1.09.06 E/F OUTLOOK 2003 (Short Course II) - Calendar, Tasks and Notes 3 1.09.06 E/F CERN EDMS - Introduction 8 5.09.06 E CERN EDMS MTF en pratique 4 6.09.06 F LabVIEW Basics 1 24 11-13.09.06 E ANSYS Workbench 32 12-15.09.06 F CERN EDMS for Engineers 8 12.09.06 E Software Engineering in the Small and the Large 16 12-13.09.06 E LabVIEW Basics 2 16 14-15.09.06 E EXCEL 2003 (Short Course I) - HowTo... Work with formulae 3 15.09.06 E/F WORD 2003 (Short Course III) - HowTo... Work with long docu...

  19. Technical Training: Places available

    CERN Multimedia

    DAvide Vitè

    2006-01-01

    Places available as of 25.7.2006 (August-December course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Titre Heure Date Langue CERN EDMS for Local Administrators 16 1-2.08.06 E ANSYS DesignModeler 16 29-30.08.06 F EXCEL 2003 - niveau 1 : ECDL 16 30-31.08.06 F OUTLOOK 2003 (Short Course I) - E-mail 3 1.09.06 E/F OUTLOOK 2003 (Short Course II) - Calendar, Tasks and Notes 3 1.09.06 E/F CERN EDMS - Introduction 8 5.09.06 E CERN EDMS MTF en pratique 4 6.09.06 F ANSYS Workbench 32 12-15.09.06 F CERN EDMS for Engineers 8 12.09.06 E Software Engineering in the Small and the Large 16 12-13.09.06 E LabVIEW Basics 2 16 14-15.09.06 E WORD 2003 (Short Course III) - HowTo... Work with long documents 3 15.09.06 E/F EXCEL 2003 (Short Course I) - HowTo... Wor...

  20. Favorite places: spatial and temporal dimensions of place attachment

    OpenAIRE

    Wood, Stephen

    2015-01-01

    From the editor: "Independent researcher Stephen Wood probes his interest in place attachment by examining two lived dialectics: the spatial tension between inward and outward aspects of place; and the temporal tension between repetitive and singular events relating to place."

  1. Take Three: Seasonal Flu

    Centers for Disease Control (CDC) Podcasts

    2010-09-29

    In this podcast, Dr. Joe Bresee describes how to keep from getting seasonal flu and spreading it to others by taking these three steps.  Created: 9/29/2010 by National Center for Immunization and Respiratory Diseases (NCIRD).   Date Released: 9/29/2010.

  2. Mathematics of Risk Taking

    Indian Academy of Sciences (India)

    https://www.ias.ac.in/article/fulltext/reso/014/01/0066-0079. Keywords. Investor; risk taking; fair game; martingale; random walk; gambling; population. Author Affiliations. K B Athreya1 2 M G Nadkarni3. Department of Mathematics Iowa State University, Ames, Iowa; I M I, Department of Mathematics, Indian Institute of Science, ...

  3. Rapid inventory taking system

    International Nuclear Information System (INIS)

    Marsden, P.S.S.F.

    1980-01-01

    A data processing system designed to facilitate inventory taking is described. The process depends upon the earliest possible application of computer techniques and the elimination of manual operations. Data is recorded in optical character recognition (OCR) 'A' form and read by a hand held wand reader. Limited validation checks are applied before recording on mini-tape cassettes. 5 refs

  4. Simulating Price-Taking

    Science.gov (United States)

    Engelhardt, Lucas M.

    2015-01-01

    In this article, the author presents a price-takers' market simulation geared toward principles-level students. This simulation demonstrates that price-taking behavior is a natural result of the conditions that create perfect competition. In trials, there is a significant degree of price convergence in just three or four rounds. Students find this…

  5. Taking of history

    DEFF Research Database (Denmark)

    Langebæk, Rikke

    2007-01-01

    Learning how to take a history is an extremely important discipline in the education of veterinary students. In our opinion the fact that this discipline is often neglected in traditional teaching is a big mistake. The mere thought of facing a real client can be almost paralysing to even the smar......Learning how to take a history is an extremely important discipline in the education of veterinary students. In our opinion the fact that this discipline is often neglected in traditional teaching is a big mistake. The mere thought of facing a real client can be almost paralysing to even...... the smartest student. So the more familiar a student can become with these situations the better. Since september 2006, veterinary students at Faculty of Life Sciences, University of Copenhagen, have received training in the discipline of history taking, using innovative educational methods: Online...... The students prepare themselves for the course by going online at home the day before class. Here they find a narrated PowerPoint presentation containing : 1) The principles of history taking 2) Client diversities – and the obstacles one might have to face with these different types of clients Video In class...

  6. Taking minutes of meetings

    CERN Document Server

    Gutmann, Joanna

    2016-01-01

    aking Minutes of Meetings guides you through the entire process behind minute taking: arranging the meeting; writing the agenda; creating the optimum environment; structuring the meeting and writing notes up accurately. The minute-taker is one of the most important and powerful people in a meeting and you can use this opportunity to develop your knowledge, broaden your horizons and build credibility within the organization. Taking Minutes of Meetings is an easy to read 'dip-in, dip-out' guide which shows you how to confidently arrange meetings and produce minutes. It provides hands-on advice about the sections of a meeting as well as tips on how to create an agenda, personal preparation, best practice advice on taking notes and how to improve your accuracy. Brand new chapters of this 4th edition include guidance on using technology to maximize effectiveness and practical help with taking minutes for a variety of different types of meetings. The creating success series of books... With over one million copi...

  7. Matters of place : the making of place and identity

    OpenAIRE

    Daniels, Jen; Baldacchino, Godfrey; Vodden, Kelly; Vodden, Kelly; Baldacchino, Godfrey; Gibson, Ryan

    2015-01-01

    Place is a key factor influencing individual and social behaviour, modes of living, and well-being (Halseth et al., 2010). Place-based development frameworks address this empirical imperative by their sensitivity to the existing assets and challenges experienced in a place. Some scholars and development practitioners recognize that “competitive advantage” comes from assets and resources nested in place. Others are drawn to place and place-making in response to fears of homog...

  8. Experiencing the enchantment of place and mobility

    DEFF Research Database (Denmark)

    Bærenholdt, Jørgen Ole

    2016-01-01

    that experiences (Erlebnisse) are practised as living adventures, where intersecting worlds are not apart from each other. These practices are performed in restless mobilities among places, where the connections and hints between place and mobility are central in making absence-presence tensions produce......Experiences of place and mobility play central roles not only in what was traditionally understood as tourism, but also in the broader practices of travelling and visiting sites and sights. On the one hand, such experiences are performed to an extent where it is difficult to isolate the sites...... and movements experienced per se, since visitors and travellers take part in ‘doing’ places and mobility. On the other, experience sites and routes stand out with specific traces and characteristics affording some – and not other – experiences. This paper discusses conceptual understandings that may help...

  9. Emerging place image

    DEFF Research Database (Denmark)

    Ooi, Can-Seng; Peji´c Kristensen, Tatjana; Lomanová Pedersen, Zdenka

    2004-01-01

    and perceptions.This paper introduces the concept of the orientalist tourist gaze, and demonstrates howorientalism may manifest in tourism. Data on how these two countries are imagined werecollected in Denmark.Keywords: destination identity, host society-guest interaction, impact of tourism, orientalism......Tourism offers an arena through which a place identity is imagined, negotiated and contained.This paper compares the Czech Republic and Slovakia, and show how these countriesconstruct and assert their identities through tourism. They both share a common history asCzechoslovakia, however...

  10. Signs in Place

    DEFF Research Database (Denmark)

    Hamid, Salmiah Binti Abdul; Jensen, Ole B.; Silva, Victor

    Travelling in unfamiliar areas is usually very interesting, however it can also be stressful. People travel or move around in an urban space according to their needs, and the environment can also influence the way people move about from one place to another. If a person gets lost, a map or GPS can...... and geosemiotic studies with regards to the road traffic signs used in urban spaces. The paper ends with a discussion on how people choreograph their movement in their everyday life from two different perspectives: above vs. below....

  11. Signs In Place

    DEFF Research Database (Denmark)

    Hamid, Salmiah Binti Abdul; Jensen, Ole B.; Silva, Victor

    2012-01-01

    Travelling in unfamiliar areas is usually very interesting; however, it can also be stressful. People travel or move around in an urban space according to their needs, and the environment can influence the way people move about from one place to another. If a person gets lost, a map or GPS can...... and geosemiotic studies with regards to the road traffic signs used in urban spaces. The paper ends with a discussion on how people choreograph their movement in their everyday life from two different perspectives: above vs. below...

  12. Tourism Sociabilities and Place

    DEFF Research Database (Denmark)

    Bødker, Mads; Browning, David

    2013-01-01

    Proposing new design opportunities, this paper challenges received notions of tourism, arguing that tourism is fundamentally social and concerned with making place. This turn makes tourism not only a convenient testing ground for technology concepts, but increasingly also for more sensitive...... renderings of, and interventions in, tourism as a relational and social practice. Using examples from commercial, arts, and design projects, and providing excerpts from our own fieldwork and design workshops with tourists and locals, this paper outlines three challenges through a conceptual lens that we see...... as productive for appropriate interaction design of tourism technologies....

  13. The Conserved Actinobacterial Two-Component System MtrAB Coordinates Chloramphenicol Production with Sporulation in Streptomyces venezuelae NRRL B-65442

    Directory of Open Access Journals (Sweden)

    Nicolle F. Som

    2017-06-01

    Full Text Available Streptomyces bacteria make numerous secondary metabolites, including half of all known antibiotics. Production of antibiotics is usually coordinated with the onset of sporulation but the cross regulation of these processes is not fully understood. This is important because most Streptomyces antibiotics are produced at low levels or not at all under laboratory conditions and this makes large scale production of these compounds very challenging. Here, we characterize the highly conserved actinobacterial two-component system MtrAB in the model organism Streptomyces venezuelae and provide evidence that it coordinates production of the antibiotic chloramphenicol with sporulation. MtrAB are known to coordinate DNA replication and cell division in Mycobacterium tuberculosis where TB-MtrA is essential for viability but MtrB is dispensable. We deleted mtrB in S. venezuelae and this resulted in a global shift in the metabolome, including constitutive, higher-level production of chloramphenicol. We found that chloramphenicol is detectable in the wild-type strain, but only at very low levels and only after it has sporulated. ChIP-seq showed that MtrA binds upstream of DNA replication and cell division genes and genes required for chloramphenicol production. dnaA, dnaN, oriC, and wblE (whiB1 are DNA binding targets for MtrA in both M. tuberculosis and S. venezuelae. Intriguingly, over-expression of TB-MtrA and gain of function TB- and Sv-MtrA proteins in S. venezuelae also switched on higher-level production of chloramphenicol. Given the conservation of MtrAB, these constructs might be useful tools for manipulating antibiotic production in other filamentous actinomycetes.

  14. Sporulation Temperature Reveals a Requirement for CotE in the Assembly of both the Coat and Exosporium Layers of Bacillus cereus Spores.

    Science.gov (United States)

    Bressuire-Isoard, Christelle; Bornard, Isabelle; Henriques, Adriano O; Carlin, Frédéric; Broussolle, Véronique

    2016-01-01

    The Bacillus cereus spore surface layers consist of a coat surrounded by an exosporium. We investigated the interplay between the sporulation temperature and the CotE morphogenetic protein in the assembly of the surface layers of B. cereus ATCC 14579 spores and on the resulting spore properties. The cotE deletion affects the coat and exosporium composition of the spores formed both at the suboptimal temperature of 20°C and at the optimal growth temperature of 37°C. Transmission electron microscopy revealed that ΔcotE spores had a fragmented and detached exosporium when formed at 37°C. However, when produced at 20°C, ΔcotE spores showed defects in both coat and exosporium attachment and were susceptible to lysozyme and mutanolysin. Thus, CotE has a role in the assembly of both the coat and exosporium, which is more important during sporulation at 20°C. CotE was more represented in extracts from spores formed at 20°C than at 37°C, suggesting that increased synthesis of the protein is required to maintain proper assembly of spore surface layers at the former temperature. ΔcotE spores formed at either sporulation temperature were impaired in inosine-triggered germination and resistance to UV-C and H2O2 and were less hydrophobic than wild-type (WT) spores but had a higher resistance to wet heat. While underscoring the role of CotE in the assembly of B. cereus spore surface layers, our study also suggests a contribution of the protein to functional properties of additional spore structures. Moreover, it also suggests a complex relationship between the function of a spore morphogenetic protein and environmental factors such as the temperature during spore formation. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  15. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: LabVIEW Real-Time (F) : 7 - 9.6.2005 (3 jours) LabVIEW Migration 6 to 7: 14.6.2005 (1 day) FrontPage 2003 - niveau 2 : 16 & 17.6.2005 (2 jours) Utilisation de fichiers PDF avec ACROBAT 6.0 : 20.6.2005 (1journée) Introduction to ANSYS: 21 - 24.6.2005 (4 days) WORD 2003 (Short Course I) - HowTo... Work with repetitive tasks /AutoText, AutoFormat, AutoCorrect, Find/Replace) : 4.7.2005 (afternoon) WORD 2003 (Short Course II) - HowTo... Mail merge: 5.7.2005 (afternoon) WORD 2003 (Short Course III) - HowTo... Work with long documents : 6.7.2005 (afternoon) ACCESS 2003 - Level 2 - ECDL AM5: 5 - 8.7.2005 (4 mornings) EXCEL 2003 (Short Course I) - HowTo... Work with formulae: 7.7.2005 (afternoon) EXCEL 2003 (Short Course II) - HowTo... Format your worksheet for printing: 8.7.2005 (aftern...

  16. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: LabVIEW base 1 : 4 - 6.7.2005 (3 jours) LabVIEW Basics 2: 7 - 8.7.2005 (2 days) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.7.2005 (1 jour) FrontPage 2003 - niveau 1 : 6-7.7.2005 (2 jours) WORD 2003 (Short Course I) - HowTo... Work with repetitive tasks /AutoText, AutoFormat, AutoCorrect, Find/Replace) : 4.7.2005 (afternoon) WORD 2003 (Short Course II) - HowTo... Mail merge: 5.7.2005 (afternoon) WORD 2003 (Short Course III) - HowTo... Work with long documents : 6.7.2005 (afternoon) OUTLOOK (Short Course I) - E-mail: 6.7.2005 (morning) OUTLOOK (Short Course II) - Calendar, Tasks and Notes: 7.7.2005 (morning) OUTLOOK (Short Course III) - Meetings and Delegation: 8.7.2005 (morning) EXCEL 2003 (Short Course I) - HowTo... Work with formulae: 7.7.2005 (afternoon) EXCEL 2003 (Short Course II) - HowTo... Format y...

  17. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: JAVA 2 Enterprise Edition - Part 1: WEB Applications: 21 & 22.2.2005 (2 days) FrontPage 2003 - niveau 1 : 21 & 22.2.2005 (2 jours) JAVA 2 Enterprise Edition - Part 2: Enterprise JavaBeans: 23 - 25.2.2005 (3 days) Utilisation des fichiers PDF avec ACROBAT 6.0 : 1.3.2005 (1 journée) ELEC-2005 - Spring Term: Integrated circuits and VLSI technology for physics: 1 - 17.3.2005 (6 X 2.30-hours lectures) C++ for Particle Physicists: 7 - 11.3.2005 (6 X 3-hour lectures) Joint PVSS JCOP Framework : 14 - 18.3.2005 (5 days) AutoCAD 2002 - niveau 1 : 15, 16, 21& 22.3.2005 (4 jours) FrontPage 2003 - niveau 2 : 21 & 22.3.2005 (2 jours) FileMaker - niveau 2 : 4 & 5.4.2005 (2 jours) EXCEL 2003 - niveau 2 : 11 & 12.4.2005 (2 jours) LabVIEW intermediate 1: 11 - 13....

  18. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Utilisation des fichiers PDF avec ACROBAT 6.0 : 1.3.2005 (1 journée) ELEC-2005 - Spring Term: Integrated circuits and VLSI technology for physics: 1 - 17.3.2005 (6 x 2.5-hour lectures) C++ for Particle Physicists: 7 - 11.3.2005 (6 x 3-hour lectures) Joint PVSS JCOP Framework : 14 - 18.3.2005 (5 days) AXEL-2005; Introduction to Particle Accelerators : 14- 18.3.2005 (10 x 1 h lectures) ACCESS 2003 - Level 1: ECDL M5: 15 - 16.3.2005 (2 days) AutoCAD 2002 - niveau 1 : 15, 16, 21& 22.3.2005 (4 jours) FrontPage 2003 - niveau 2 : 21 & 22.3.2005 (2 jours) Hands-on Object-Oriented Design and Programming with C++ : 22 - 24.3.2005 (3 days) FileMaker - niveau 2 : 4 & 5.4.2005 (2 jours) EXCEL 2003 - niveau 2 : 11 & 12.4.2005 (2 jours) LabVIEW Intermediate 1: 11 - 13.4.2005...

  19. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: LabVIEW Real-Time (F) : 7 - 9.6.2005 (3 jours) LabVIEW Migration 6 to 7: 14.6.2005 (1 day) IT3T/1 - Read your mail and more with Outlook 2003 : 14.6.2005 (IT Technical Training Tutorial, free of charge) IT3T/2 - Creating, managing and using distribution lists with Simba2 : 16.6.2005 (IT Technical Training Tutorial, free of charge) FrontPage 2003 - niveau 2 : 16 & 17.6.2005 (2 jours) Utilisation de fichiers PDF avec ACROBAT 6.0 : 20.6.2005 (1journée) Introduction to ANSYS: 21 - 24.6.2005 (4 days) IT3T/3 - Working remotely with Windows XP : 28.6.2005 (IT Technical Training Tutorial, free of charge) IT3T/4 - Editing Websites with Frontpage 2003 : 30.6.2005 (IT Technical Training Tutorial, free of charge) WORD 2003 (Short Course I) - HowTo... Work with repetitive tasks /AutoText, AutoFormat, AutoC...

  20. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Title Hours Date Language ACCESS 2003 - Level 2: ECDL AM5 16 19 to 20-01-06 E-F AutoCAD 2006 - niveau 1 32 19 to 25-01-06 F C++ Programming Advanced - Traps and Pitfalls 32 24 to 27-01-06 E STEP7 : Level 1 32 24 to 27-01-06 E LabVIEW Basics 2 16 26 to 27-01-06 E AutoCAD Mechanical 2006 16 30 to 31-01-06 F DIAdem : base 24 01 to 03-02-06 F FrontPage 2003 - niveau 1 16 02 to 03-02-06 F ACROBAT 7.0 : Utilisation de fichiers PDF 8 06-02-06 F Manipulation des images 4 08-02-06 F OUTLOOK 2003 (Short Course I) - E-mail 3 09-02-06 E-F WORD 2003 (Short Course II) - HowTo... Mail merge 3 09-02-06 E-F ACCESS 2003 - Level 1: ECDL M5 16 13 to 14-02-06 E-F JCOP: Control System Integration using JCOP Tools 24 14 to 16-02-06 E OUTLOOK 2003 (Short Course II) - Calendar, Tasks and Note...

  1. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: JAVA 2 Enterprise Edition - Part 1: WEB Applications: 21 & 22.2.2005 (2 days) FrontPage 2003 - niveau 1 : 21 & 22.2.2005 (2 jours) JAVA 2 Enterprise Edition - Part 2: Enterprise JavaBeans: 23 - 25.2.2005 (3 days) Utilisation des fichiers PDF avec ACROBAT 6.0 : 1.3.2005 (1 journée) Hands-on Object-Oriented Design and Programming with C++ : 1 - 3.3.2005 (3 days) ELEC-2005 - Spring Term: Integrated circuits and VLSI technology for physics: 1 - 17.3.2005 (6 x 2.5-hour lectures) C++ for Particle Physicists: 7 - 11.3.2005 (6 x 3-hour lectures) Joint PVSS JCOP Framework : 14 - 18.3.2005 (5 days) ACCESS 2003 - Level 1 : 15 - 16.3.2005 (2 days) AutoCAD 2002 - niveau 1 : 15, 16, 21& 22.3.2005 (4 jours) FrontPage 2003 - niveau 2 : 21 & 22.3.2005 (2 jours) FileMaker - ...

  2. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Joint PVSS JCOP Framework: 8 - 12.8.2005 (5 days) WORD 2003 (Short Course I) - HowTo... Work with AutoTasks (AutoText, AutoFormat, AutoCorrect, Lists, Find/Replace): 25.8.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 25.8.2005 (afternoon) EXCEL 2003 (Short Course I) - HowTo... Work with formulae: 26.8.2005 (morning) WORD 2003 (Short Course III) - HowTo... Work with long documents: 26.8.2005 (afternoon) FrontPage 2003 - niveau 1 : 1 - 2.9.2005 (2 jours) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.9.2005 (1 jour) LabVIEW Basics 1 : 5 - 7.9.2005 (3 days, dates to be confirmed) Introduction à Windows XP au CERN : 12.9.2005 (1 demi-journée) FrontPage 2003 - niveau 2 : 15 - 16.9.2005 (2 jours) AutoCAD 2005 - niveau 1 : 22, 23, 28, 29.9.2005 (4 jours) MAGNE-05 - Magn&...

  3. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Hands-on Introduction to Python Programming: 28 - 30.6.2005 (3 days) Introduction to ANSYS: 28.6 - 1.7.2005 (4 days) IT3T/3 - Working remotely with Windows XP: 28.6.2005 (IT Technical Training Tutorial, free of charge) IT3T/4 - Editing Websites with Frontpage 2003: 30.6.2005 (IT Technical Training Tutorial, free of charge) LabVIEW base 1 : 4 - 6.7.2005 (3 jours) LabVIEW Basics 2: 7 - 8.7.2005 (2 days) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.7.2005 (1 jour) FrontPage 2003 - niveau 1 : 6-7.7.2005 (2 jours) WORD 2003 (Short Course I) - HowTo... Work with repetitive tasks /AutoText, AutoFormat, AutoCorrect, Find/Replace) : 4.7.2005 (afternoon) WORD 2003 (Short Course II) - HowTo... Mail merge: 5.7.2005 (afternoon) WORD 2003 (Short Course III) - HowTo... Work with long documents : 6.7.2005 (afternoon) ACCES...

  4. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Joint PVSS JCOP Framework: 8 - 12.8.2005 (5 days) FrontPage 2003 - niveau 1 : 1 - 2.9.2005 (2 jours) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.9.2005 (1 jour) LabVIEW Basics 1 : 5 - 7.9.2005 (3 days, dates to be confirmed) Introduction à Windows XP au CERN : 12.9.2005 (1 demi-journée) FrontPage 2003 - niveau 2 : 15 - 16.9.2005 (2 jours) AutoCAD 2005 - niveau 1 : 22, 23, 28, 29.9.2005 (4 jours) MAGNE-05 - Magnétisme pour l'électrotechnique : 27 - 29.9.2005 (3 jours) LabVIEW Application Development (Intermediate I): 5 - 7.12.2005 (3 days) LabVIEW Advanced Programming (Intermediate 2): 8 - 9.12.2005 (2 days) ENSEIGNEMENT TECHNIQUE TECHNICAL TRAINING Monique Duval 74924 technical.training@cern.ch

  5. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: FrontPage 2003 - niveau 1 : 9 & 10.5.2005 (2 jours) ELEC-2005 - Summer Term: System electronics for physics - Issues : 10, 12, 17, 19, 24, 26 & 31.5.2005 (7 x 2h lectures) AutoCAD 2005 - niveau 1 : 12, 13, 18, 19.5.2005 (4 jours) ACCESS 2003 - Level 1: ECDL M5 : 11 & 12.5.2005 (2 days) Object-Oriented Analysis and Design using UML: 17 - 19.5.2005 (3 days) Synplify Pro Training: 18.5.2005 (1 day) Finite State Machines in the JCOP Framework: 24 - 26.5.2005 (3 days) The Joint PVSS JCOP Framework: 30.5 - 3.6.2005 (5 days) Introduction à la CAO CADENCE : 31.5 - 1.6.2005 (2 jours) LabVIEW Real-Time (F) : 7 - 9.6.2005 (3 jours) LabVIEW Migration 6 to 7: 14.6.2005 (1 day) FrontPage 2003 - niveau 2 : 16 & 17.6.2005 (2 jours) Utilisation de fichiers PDF avec ACROBAT 6.0 : 20.6.2005 (1journée) Intr...

  6. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: FrontPage 2003 - niveau 1 : 9 & 10.5.2005 (2 jours) ELEC-2005 - Summer Term: System electronics for physics - Issues : 10, 12, 17, 19, 24, 26 & 31.5.2005 (7 x 2h lectures) AutoCAD 2005 - niveau 1 : 11, 12, 18, 19.5.2005 (4 jours) Object-Oriented Analysis and Design using UML: 17 - 19.5.2005 (3 days) Synplify Pro Training: 18.5.2005 (1 day) Finite State Machines in the JCOP Framework: 24 - 26.5.2005 (3 days) The Joint PVSS JCOP Framework: 30.5 - 3.6.2005 (5 days) Introduction à la CAO CADENCE : 31.5 - 1.6.2005 (2 jours) LabVIEW Real-Time (F) : 7 - 9.6.2005 (3 jours) LabVIEW Migration 6 to 7: 14.6.2005 (1 day) FrontPage 2003 - niveau 2 : 16 & 17.6.2005 (2 jours) Utilisation de fichiers PDF avec ACROBAT 6.0 : 20.6.2005 (1journée) Introductio...

  7. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Hands-on Introduction to Python Programming: 28 - 30.6.2005 (3 days) Introduction to ANSYS: 21 - 24.6.2005 (4 days) IT3T/3 - Working remotely with Windows XP: 28.6.2005 (IT Technical Training Tutorial, free of charge) IT3T/4 - Editing Websites with Frontpage 2003: 30.6.2005 (IT Technical Training Tutorial, free of charge) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.7.2005 (1 jour) FrontPage 2003 - niveau 1 : 6-7.7.2005 (2 jours) LabVIEW base 1 : 4 - 6.7.2005 (3 jours) LabVIEW Basics 2: 7 - 8.7.2005 (2 days) WORD 2003 (Short Course I) - HowTo... Work with repetitive tasks /AutoText, AutoFormat, AutoCorrect, Find/Replace) : 4.7.2005 (afternoon) WORD 2003 (Short Course II) - HowTo... Mail merge: 5.7.2005 (afternoon) WORD 2003 (Short Course III) - HowTo (4 mornings) EXCEL 2003 (Short Course I) - HowTo... Work ...

  8. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Title Hours Date Language AutoCAD Mechanical 2006 16 30 to 31-01-06 F DIAdem : base 24 01 to 03-02-06 F ACROBAT 7.0 : Utilisation de fichiers PDF 8 06-02-06 F Manipulation des images 4 08-02-06 F OUTLOOK 2003 (Short Course I) - E-mail 3 09-02-06 E-F WORD 2003 (Short Course II) - HowTo... Mail merge 3 09-02-06 E-F ACCESS 2003 - Level 1: ECDL M5 16 13 to 14-02-06 E-F JCOP: Control System Integration using JCOP Tools 24 14 to 16-02-06 E OUTLOOK 2003 (Short Course II) - Calendar, Tasks and Notes 3 16-02-06 E-F WORD 2003 (Short Course III) - HowTo... Work with long documents 3 16-02-06 E-F FrontPage 2003 - niveau 1 16 23 to 24-02-06 F OUTLOOK 2003 (Short Course III) - Meetings and Delegation 3 27-02-06 E-F WORD 2003 (Short Course IV) - HowTo... Work with master document 3 27-02-06 E-F ...

  9. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Joint PVSS JCOP Framework: 8 - 12.8.2005 (5 days) WORD 2003 (Short Course I) - HowTo... Work with AutoTasks (AutoText, AutoFormat, AutoCorrect, Lists, Find/Replace): 25.8.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 25.8.2005 (afternoon) EXCEL 2003 (Short Course I) - HowTo... Work with formulae: 26.8.2005 (morning) WORD 2003 (Short Course III) - HowTo... Work with long documents: 26.8.2005 (afternoon) FrontPage 2003 - niveau 1 : 1 - 2.9.2005 (2 jours) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.9.2005 (1 jour) LabVIEW Basics 1 : 5 - 7.9.2005 (3 days, dates to be confirmed) Introduction à Windows XP au CERN : 12.9.2005 (1 demi-journée) FrontPage 2003 - niveau 2 : 15 - 16.9.2005 (2 jours) AutoCAD 2005 - niveau 1 : 22, 23, 28, 29.9.2005 (4 jours) Finite State Machines in the JCOP Framew...

  10. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The JAVA Programming Language Level 1: 11 & 12.1.2005 (2 days) Introduction to XML : 13 & 14.1.2005 (2 days) The CERN EDMS for Local Administrators: 19 & 20.1.2005 (2 days - free course) Programmation Unity-Pro pour utilisateurs de Schneider PL7-Pro : 24 - 28.1.2005 (8 demi-journées) LabVIEW base 1/basics 1 : 31.1 - 1.2.2005 (2 j size="2">LabVIEW base 2/basics 2 : 3 & 4.2.2005 (2 jours/2 days - langue à décider/language to be decided) Finite State Machines in the JCOP Framework: 1 - 3.2.2005 (3 days - free course) The JAVA Programming Language Level 2: 7 - 9.2.2005 (3 days) C++ Programming Advanced -Traps and Pitfalls: 8 - 11.2.2005 (4 days) Joint PVSS JCOP Framework: 14 - 18.2.2005 (5 days) JAVA 2 Enterprise Edition - Part 1: ...

  11. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The JAVA Programming Language Level 1: 11 & 12.1.2005 (2 days) Introduction to XML : 13 & 14.1.2005 (2 days) ELEC-2005.:Winter term: Introduction to electronics in HEP: 18, 20, 25, 27.1, 1 & 3.2.2005 (6 x 2h lectures) The CERN EDMS for Local Administrators: 19 & 20.1.2005 (2 days - free course) Programmation Unity-Pro pour utilisateurs de Schneider PL7-Pro : 24 - 28.1.2005 (8 demi-journées) CLEAN-2002 : Travailler en salle propre : 25.1.2005 (après-midi, cours gratuit) Compatibilité électromagnétique (CEM) : installation et remèdes : 25 - 27.1.2005 (3 jours) Finite State Machines in the JCOP Framework: 1 - 3.2.2005 (3 days - free course) The JAVA Programming Language Level 2: 7 - 9...

  12. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: ELEC-2005 - Spring Term: Integrated circuits and VLSI technology for physics: 1 - 17.3.2005 (6 x 2.5-hour lectures) C++ for Particle Physicists: 7 - 11.3.2005 (6 x 3-hour lectures) Joint PVSS JCOP Framework : 14 - 18.3.2005 (5 days) Oracle 9i: SQL: 14 -16.3.2005 (3 days) AXEL-2005; Introduction to Particle Accelerators : 14- 18.3.2005 (10 x 1 h lectures) ACCESS 2003 - Level 1: ECDL M5: 15 - 16.3.2005 (2 days) AutoCAD 2002 - niveau 1 : 15, 16, 21& 22.3.2005 (4 jours) FrontPage 2003 - niveau 2 : 21 & 22.3.2005 (2 jours) Hands-on Object-Oriented Design and Programming with C++ : 22 - 24.3.2005 (3 days) FileMaker - niveau 2 : 4 & 5.4.2005 (2 jours) Oracle 9i: Programming with PL/SQL: 4 - 6.4.2005 (3 days) EXCEL 2003 - niveau 2 : 11 & 12.4.2005 (2 jours) LabVIEW Intermedia...

  13. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: LabVIEW Intermediate 1: 11 - 13.4.2005 (3 days) ACCESS 2003 - Level 2 - ECDL AM5: 13 & 14.4.2005 (2 days) LabVIEW Intermediate 2: 14 & 15.4.2005 (2 days) PowerPoint 2003 (F) : 25.4.2005 (1 jour) WORD 2003 - niveau 1 : 2 & 3.5.2005 (2 jours) FrontPage 2003 - niveau 1 : 9 & 10.5.2005 (2 jours) ANSYS Workbench (F) : 9 - 12.5.2005 (4 jours) ELEC-2005 - Summer Term: System electronics for physics - Issues : 10, 12, 17, 19, 24, 26 & 31.5.2005 (7 x 2h lectures) La technique du vide : 12 & 13.5.2005 (2 jours) Finite State Machines in the JCOP Framework: 24 - 26.5.2005 (3 days) The Joint PVSS JCOP Framework ; 30.5 - 3.6.2005 (5 days) LabVIEW Migration 6 to 7: 14.6.2005 (1 day) Introduction to ANSYS: 21 - 24.6.2005 (4 days) ENSEIGNEMENT ...

  14. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: PowerPoint 2003 (F) : 25.4.2005 (1 jour) WORD 2003 - niveau 1 : 2 & 3.5.2005 (2 jours) FrontPage 2003 - niveau 1 : 9 & 10.5.2005 (2 jours) ANSYS Workbench (F) : 9 - 12.5.2005 (4 jours) ELEC-2005 - Summer Term: System electronics for physics - Issues : 10, 12, 17, 19, 24, 26 & 31.5.2005 (7 x 2h lectures) AutoCAD 2005 - niveau 1 : 11, 12, 18, 19.5.2005 (4 jours) ACCESS 2003 - Level 1: ECDL M5 : 11 & 12.5.2005 (2 days) La technique du vide : 12 & 13.5.2005 (2 jours) Finite State Machines in the JCOP Framework: 24 - 26.5.2005 (3 days) The Joint PVSS JCOP Framework: 30.5 - 3.6.2005 (5 days) LabVIEW Migration 6 to 7: 14.6.2005 (1 day) Introduction to ANSYS: 21 - 24.6.2005 (4 days) ENSEIGNEMENT TECHNIQUE TECHNICAL TRAINING Monique Duval 74924 tech...

  15. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: ACCESS 2003 - Level 1: ECDL M5: 15 - 16.3.2005 (2 days) Hands-on Object-Oriented Design and Programming with C++ : 22 - 24.3.2005 (3 days) FileMaker - niveau 2 : 4 & 5.4.2005 (2 jours) Oracle 9i: Programming with PL/SQL: 4 - 6.4.2005 (3 days) EXCEL 2003 - niveau 2 : 11 & 12.4.2005 (2 jours) LabVIEW Intermediate 1: 11 - 13.4.2005 (3 days) ACCESS 2003 – Level 2 – ECDL AM5: 13 & 14.4.2005 (2 days) LabVIEW Intermediate 2: 14 & 15.4.2005 (2 days) PowerPoint 2003 (F) : 18.4.2005 (1 jour) Joint PVSS JCOP Framework : 25 – 29.4.2005 (5 days) ELEC-2005 - Summer Term: System electronics for physics - Issues : 10, 12, 17, 19, 24, 26 & 31.5.2005 (7 x 2h lectures) AutoCAD 2002 – niveau 1 : 11, 12, 18 & 19.5.2005 (4 jours) LabVIEW M...

  16. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: LabVIEW base 2/basics 2 : 3 & 4.2.2005 (2 jours/2 days - langue à décider/language to be decided) Finite State Machines in the JCOP Framework: 1 - 3.2.2005 (3 days - free course) The JAVA Programming Language Level 2: 7 - 9.2.2005 (3 days) JAVA 2 Enterprise Edition - Part 1: WEB Applications: 21 & 22.2.2005 (2 days) FrontPage 2003 - niveau 1 : 21 & 22.2.2005 (2 jours) JAVA 2 Enterprise Edition - Part 2: Enterprise JavaBeans: 23 - 25.2.2005 (3 days) ELEC-2005 - Spring Term: Integrated circuits and VLSI technology for physics: 1 - 17.3.2005 (6 X 2.30-hours lectures) C++ for Particle Physicists: 7 - 11.3.2005 (6 X 3-hour lectures) Joint PVSS JCOP Framework : 14 - 18.3.2005 (5 days) AutoCAD 2002 - niveau 1 : 15, 16, 21& 22.3.2005 (4 jours) FrontPage 20...

  17. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Hands-on Object-Oriented Design and Programming with C++ : 22 - 24.3.2005 (3 days) FileMaker - niveau 2 : 4 & 5.4.2005 (2 jours) EMAG-2004 - Electromagnetic Design and Mathematical Optimization in Magnet Technology: 4- 14.4.2005 (8 x 3h) EXCEL 2003 - niveau 2 : 11 & 12.4.2005 (2 jours) LabVIEW Intermediate 1: 11 - 13.4.2005 (3 days) ACCESS 2003 - Level 2 - ECDL AM5: 13 & 14.4.2005 (2 days) LabVIEW Intermediate 2: 14 & 15.4.2005 (2 days) PowerPoint 2003 (F) : 18.4.2005 (1 jour) Joint PVSS JCOP Framework : 25 - 29.4.2005 (5 days) WORD 2003 - niveau 1 : 2 & 3.5.2005 (2 jours) ELEC-2005 - Summer Term: System electronics for physics - Issues : 10, 12, 17, 19, 24, 26 & 31.5.2005 (7 x 2h lectures) AutoCAD 2002 - niveau 1 : 11, 12, 18 & 19.5.2005 (4 jour...

  18. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: LabVIEW Migration 6 to 7: 14.6.2005 (1 day) IT3T/1 - Read your mail and more with Outlook 2003 : 14.6.2005 (IT Technical Training Tutorial, free of charge) IT3T/2 - Creating, managing and using distribution lists with Simba2 : 16.6.2005 (IT Technical Training Tutorial, free of charge) FrontPage 2003 - niveau 2 : 16 & 17.6.2005 (2 jours) size="2">(1journée) Hands-on Introduction to Python Programming: 28 - 30.6.2005 (3 days) Introduction to ANSYS: 21 - 24.6.2005 (4 days) IT3T/3 - Working remotely with Windows XP: 28.6.2005 (IT Technical Training Tutorial, free of charge) IT3T/4 - Editing Websites with Frontpage 2003: 30.6.2005 (IT Technical Training Tutorial, free of charge) LabVIEW base 1 : 4 - 6.7.2005 (3 jours) LabVIEW Basics 2: AutoCorrect, Find/Replace) : 4.7.2005 (afternoon) WORD 2003 (Shor...

  19. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Introduction à ANSYS : 23 - 26.11.2004 (4 jours) FileMaker - niveau 1 : 23 - 26.11.2004 (4 jours) Project Planning with MS-Project : 25.11 & 2.12.2004 (2 days) Explicit Dynamics with ANSYS/LS-Dyna : 7 - 9.12.2004 (3 days) Introduction to PERL 5 : 8 & 9.12.2004 (2 days) PCAD Schémas - Débutants : 9 & 10.12.2004 (2 jours) The JAVA Programming Language Level 1 : 11 & 12.1.2005 (2 days) Introduction to XML : 13 & 14.1.2005 (2 days) Introduction to the CERN EDMS : 18.1.2005 (1 day - free course) The CERN EDMS for Local Administrators : 19 & 20.1.2005 (2 days - free course) Programmation Unity-Pro pour utilisateurs de Schneider PL7-Pro : 24 - 28.1.2005 (8 demi-journées) CLEAN-2002 : Travaill...

  20. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Explicit Dynamics with ANSYS/LS-Dyna : 7 - 9.12.2004 (3 days) Introduction to PERL 5 : 8 & 9.12.2004 (2 days) PCAD Schémas - Débutants : 9 & 10.12.2004 (2 jours) Advanced aspects of PERL 5: 10.12.2004 (1 day) PCAD PCB – Débutants : 13 - 15.12.2004 (3 jours) The JAVA Programming Language Level 1 : 11 & 12.1.2005 (2 days) Introduction to XML : 13 & 14.1.2005 (2 days) Introduction to the CERN EDMS : 18.1.2005 (1 day - free course) The CERN EDMS for Local Administrators : 19 & 20.1.2005 (2 days - free course) Programmation Unity-Pro pour utilisateurs de Schneider PL7-Pro : 24 - 28.1.2005 (8 demi-journées) CLEAN-2002 : Travailler en salle propre : 25.1.2005 (après-midi, cours gratuit) Compatibilit&eac...

  1. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: C++ for Particle Physicists : 15 - 19.11.2004 (6 X 3 hours sessions) Word 2003 - niveau 1 : 22 & 23.11.2004 (2 jours) Introduction à ANSYS : 23 - 26.11.2004 (4 jours) Project Planning with MS-Project : 25.11 & 2.12.2004 (2 days) Explicit Dynamics with ANSYS/LS-Dyna : 7 - 9.12.2004 (2 days) PCAD Schémas - Débutants : 9 & 10.12.2004 (2 jours) The JAVA Programming Language Level 1 : 11 & 12.1.2005 (2 days) Introduction to XML : 13 & 14.1.2005 (2 days) CLEAN-2002 : Travailler en salle propre : 25.1.2005 (après-midi, cours gratuit) Compatibilité électromagnétique (CEM) : installation et remèdes : 25 - 27.1.2005 (3 jours) Finite State Machines in the JCOP Fr ...

  2. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: FileMaker - niveau 1 : 23 - 26.11.2004 (4 jours) Explicit Dynamics with ANSYS/LS-Dyna : 7 - 9.12.2004 (3 days) Introduction to PERL 5 : 8 & 9.12.2004 (2 days) PCAD Schémas - Débutants : 9 & 10.12.2004 (2 jours) Advanced aspects of PERL 5: 10.12.2004 (1 day) PCAD PCB – Débutants : 13 - 15.12.2004 (3 jours) The JAVA Programming Language Level 1 : 11 & 12.1.2005 (2 days) Introduction to XML : 13 & 14.1.2005 (2 days) Introduction to the CERN EDMS : 18.1.2005 (1 day - free course) The CERN EDMS for Local Administrators : 19 & 20.1.2005 (2 days - free course) Programmation Unity-Pro pour utilisateurs de Schneider PL7-Pro : 24 - 28.1.2005 (8 demi-journées) CLEAN-2002 : Travailler en salle propre : 25.1.2005 ...

  3. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: FileMaker - niveau 1 :  20 - 21.10.2005 (2 jours) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) Finite State Machines in the JCOP Framework : 25 - 27.10.2005 (3 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) OUTLOOK (Short Course I) - E-mail: 2.11.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 2.11.2005 (afternoon) FrontPage 2003 - niveau 1 :  3 - 4.11.2005 (2 jours) AutoCAD 2006 - niveau 1 : 3, 4, 9, 10.11.2005 (4 jours) Joint PVSS JCOP Framework : 7 - 11.11.2005 (5 days) OUTLOOK (Short Course II) - Calendar, Tasks and Notes: 16.11.2005 (morning) Joint PVSS JCOP Framework : 21 - 25.11.2005 (5 days) OUTLOOK (Short Course III) - Meetings and Delegation: 30.11.2005 (morning) WORD 2003 (Short Course III) - HowTo... Work with long documents : ...

  4. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: The Java Programming Language Level 1: 21 - 23.11.2005 (3 days) OUTLOOK (Short Course III) - Meetings and Delegation: 30.11.2005 (morning) WORD 2003 (Short Course III) - HowTo... Work with long documents : 30.11.2005 (afternoon) FrontPage 2003 - niveau 2 : 5 - 6.12.2005 (2 jours) Introduction à ANSYS Classique : 6 - 9.12.2005 (4 jours) EXCEL 2003 (Short Course II) - HowTo... Format your worksheet for printing: 7.12.2005 (morning) PCAD Schémas - Introduction : 8 - 9.12.2005 (2 jours) ACCESS 2003 - Level 2 - ECDL AM5: 14 - 15.12.2005 (2 days) LabVIEW Basics I:  23 - 25.1.2006 (3 days) C++ Programming Advanced - Traps and Pitfalls: 24 - 27.1.2006 (4 days) LabVIEW Basics II:  26 - 27.1.2006 (2 days) Joint PVSS-JCOP Framework: 30.1 - 3.2.2006 (5 days, free of charge) Finite State Machines in the JCOP Framework : ...

  5. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Finite State Machines in the JCOP Framework:  26 - 28.9.2005 (3 days) WORD 2003 (Short Course IV) - HowTo... Work with master document: 28.9.2005 (morning) Joint PVSS JCOP Framework : 3 - 7.10.2005 (5 days) Introduction à Dreamweaver MX :  3 - 4.10.2005 (2 jours) Utilisation des fichiers PDF avec Acrobat 7.0 :   4.10.2005 (1 journée) LaTeX par la pratique :   4 - 6.10.2005 (3 matinées) PowerPoint 2003 (F) :  7.10.2005 (1 journée) FileMaker - niveau 1 :  20 - 21.10.2005 (2 jours) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) WORD 2003 (Short Course I) - HowTo... Work with AutoTasks: 26.10.2005 (afternoon) OUTLOOK (Short Course I) - E-mail: 2.11.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 2.11....

  6. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Joint PVSS JCOP Framework : 7 - 11.11.2005 (5 days) ELEC-2005 Autumn Term - Electronics applications in HEP experiments: 8.11 - 8.12.2005 (10 x 2h lectures) The EDMS-MTF in practice: 9.11.2005 (morning, free of charge) The CERN EDMS for Local Administrators: 15-16.11.2005 (2 days, free of charge) OUTLOOK (Short Course II) - Calendar, Tasks and Notes: 16.11.2005 (morning) Hands-On Object Oriented Design and Programming with C++ : 16 - 18.11.2005 (3 days) The CERN EDMS for Engineers: 17.11.2005 (1 day, free of charge) The Java Programming Language Level 1: 21 - 23.11.2005 (3 days) Hands-on Introduction to Python Programming: 28 - 30.11.2005 (3 days) OUTLOOK (Short Course III) - Meetings and Delegation: 30.11.2005 (morning) WORD 2003 (Short Course III) - HowTo... Work with long documents : 30.11.2005 (afternoon) Fron...

  7. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: OUTLOOK (Short Course III) - Meetings and Delegation: 30.11.2005 (morning) WORD 2003 (Short Course III) - HowTo... Work with long documents: 30.11.2005 (afternoon) FrontPage 2003 - niveau 2 : 5 - 6.12.2005 (2 jours) Introduction à ANSYS Classique : 6 - 9.12.2005 (4 jours) EXCEL 2003 (Short Course II) - HowTo... Format your worksheet for printing: 7.12.2005 (morning) AutoCAD - mise à jour d’AutoCAD 2002 à AutoCAD 2006 : 8.12.2005 (1 journée - pour les utilisateurs d’AutoCAD 2002) PCAD Schémas - Introduction : 8 - 9.12.2005 (2 jours) Finite State Machines in the JCOP Framework: 13 - 15.12.2005 (3 days) PCAD PCB - Introduction : 14 - 16.12.2005 (3 jours) ACCESS 2003 - Level 2 - ECDL AM5: 14 - 15.12.2005 (2 days) AutoCAD 2006 - niveau 1 : 19, 20, 24 & 25.1.2006 (4 jours) LabVIEW Basics I:  23 - 25.1.2006...

  8. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: FrontPage 2003 - niveau 2 : 5 - 6.12.2005 (2 jours) Introduction à ANSYS Classique : 6 - 9.12.2005 (4 jours) EXCEL 2003 (Short Course II) - HowTo... Format your worksheet for printing: 7.12.2005 (morning) PCAD Schémas - Introduction : 8 - 9.12.2005 (2 jours) Finite State Machines in the JCOP Framework: 13 - 15.12.2005 (3 days) ACCESS 2003 - Level 2 - ECDL AM5: 14 - 15.12.2005 (2 days) PCAD PCB - Introduction : 14 - 16.12.2005 (3 jours) AutoCAD 2006 - niveau 1 : 19, 20, 24 & 25.1.2006 (4 jours) LabVIEW Basics I:  23 - 25.1.2006 (3 days) C++ Programming Advanced - Traps and Pitfalls: 24 - 27.1.2006 (4 days) STEP7 Programming Level 1: 24 - 27.1.2006 (4 days) LabVIEW Basics II:  26 - 27.1.2006 (2 days) AutoCAD Mechanical 2006 : 30 - 31.1.2006 (2 jours; suite du cours AutoCAD 2006 - niveau 1) Joint PVS...

  9. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Introduction à Windows XP au CERN : 12.9.2005 (1 demi-journée) FrontPage 2003 - niveau 2 : 15 - 16.9.2005 (2 jours) Finite State Machines in the JCOP Framework:  26 - 28.9.2005 (3 days) MAGNE-05 - Magnétisme pour l'électrotechnique : 27 - 29.9.2005 (3 jours) WORD 2003 (Short Course IV) - HowTo... Work with master document: 28.9.2005 (morning) Joint PVSS JCOP Framework : 3 - 7.10.2005 (5 days) Introduction à Dreamweaver MX :  3 - 4.10.2005 (2 jours) Utilisation des fichiers PDF avec Acrobat 7.0 :   4.10.2005 (1 journée) LaTeX par la pratique :   4 - 6.10.2005 (3 matinées) PowerPoint 2003 (F) :  7.10.2005 (1 journée) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) WORD 2003 (Short Course I) - HowTo... Work with Auto...

  10. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: FrontPage 2003 - niveau 1 : 1 - 2.9.2005 (2 jours) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.9.2005 (1 jour) LabVIEW Basics 1:  5 â€" 7.9.2005 (3 days) Introduction à Windows XP au CERN : 12.9.2005 (1 demi-journée) FrontPage 2003 - niveau 2 : 15 - 16.9.2005 (2 jours) AutoCAD 2005 - niveau 1 : 22, 23, 28, 29.9.2005 (4 jours) Finite State Machines in the JCOP Framework:  26 - 28.9.2005 (3 days) MAGNE-05 - Magnétisme pour l'électrotechnique : 27 - 29.9.2005 (3 jours) Joint PVSS JCOP Framework : 3 - 7.10.2005 (5 days) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) OUTLOOK (Short Course I) - E-mail: 2.11.2005 (morning) OUTLOOK (Short Course II) - Calendar, Tasks and Notes: 16.11.2005 (morning) OU...

  11. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: FileMaker - niveau 1 :  20 - 21.10.2005 (2 jours) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) Finite State Machines in the JCOP Framework : 25 - 27.10.2005 (3 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) WORD 2003 (Short Course I) - HowTo... Work with AutoTasks: 26.10.2005 (afternoon) OUTLOOK (Short Course I) - E-mail: 2.11.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 2.11.2005 (afternoon) FrontPage 2003 - niveau 1 :  3 - 4.11.2005 (2 jours) AutoCAD 2006 - niveau 1 : 3, 4, 9, 10.11.2005 (4 jours) Joint PVSS JCOP Framework : 7 - 11.11.2005 (5 days) OUTLOOK (Short Course II) - Calendar, Tasks and Notes: 16.11.2005 (morning) Joint PVSS JCOP Framework : 21 - 25.11.2005 (5 days) OUTLOOK (Short Course III) - Meetings and Delegation: 30.11....

  12. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) Finite State Machines in the JCOP Framework : 25 - 27.10.2005 (3 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) Introduction to the CERN EDMS: 2.11.2005 (1 day, free of charge) OUTLOOK (Short Course I) - E-mail: 2.11.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 2.11.2005 (afternoon) FrontPage 2003 - niveau 1 :  3 - 4.11.2005 (2 jours) AutoCAD 2006 - niveau 1 : 3, 4, 9, 10.11.2005 (4 jours) Joint PVSS JCOP Framework : 7 - 11.11.2005 (5 days) The CERN EDMS for Engineers: 8.11.2005 (1 day, free of charge) The EDMS-MTF in practice: 9.11.2005 (morning, free of charge) The CERN EDMS for Local Administrators: 15-16.11.2005 (2 days, free of charge) OUTLOOK (Short Course II) - Calendar, Tasks and Notes: ...

  13. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.9.2005 (1 jour) LabVIEW Basics 1:  5 - 7.9.2005 (3 days) Introduction à Windows XP au CERN : 12.9.2005 (1 demi-journée) FrontPage 2003 - niveau 2 : 15 - 16.9.2005 (2 jours) AutoCAD 2005 - niveau 1 : 22, 23, 28, 29.9.2005 (4 jours) Finite State Machines in the JCOP Framework:  26 - 28.9.2005 (3 days) MAGNE-05 - Magnétisme pour l'électrotechnique : 27 - 29.9.2005 (3 jours) WORD 2003 (Short Course IV) - HowTo... Work with master document: 28.9.2005 (morning) Joint PVSS JCOP Framework : 3 - 7.10.2005 (5 days) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) WORD 2003 (Short Course I) - HowTo... Work with AutoTasks: 26.10.2005 (afternoon) OUTLOOK (Short Course I) - E-mail: 2...

  14. Technical training: places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Introduction to the CERN EDMS: 2.11.2005 (1 day, free of charge) OUTLOOK (Short Course I) - E-mail: 2.11.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 2.11.2005 (afternoon) FrontPage 2003 - niveau 1 :  3 - 4.11.2005 (2 jours) AutoCAD 2006 - niveau 1 : 3, 4, 9, 10.11.2005 (4 jours) Joint PVSS JCOP Framework : 7 - 11.11.2005 (5 days) The CERN EDMS for Engineers: 8.11.2005 (1 day, free of charge) The EDMS-MTF in practice: 9.11.2005 (morning, free of charge) The CERN EDMS for Local Administrators: 15-16.11.2005 (2 days, free of charge) OUTLOOK (Short Course II) - Calendar, Tasks and Notes: 16.11.2005 (morning) Hands-On Object Oriented Design and Programming with C++ : 16 - 18.11.2005 (3 days) The Java Programming Language Level 1: 21 - 23.11.2005 (3 days) Hands-on Introduction to Python Prog...

  15. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Joint PVSS JCOP Framework : 3 - 7.10.2005 (5 days) Utilisation des fichiers PDF avec Acrobat 7.0 :   4.10.2005 (1 journée) LaTeX par la pratique :   4 - 6.10.2005 (3 matinées) PowerPoint 2003 (F) :  7.10.2005 (1 journée) FileMaker - niveau 1 :  20 - 21.10.2005 (2 jours) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) Finite State Machines in the JCOP Framework : 25 - 27.10.2005 (3 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) WORD 2003 (Short Course I) - HowTo... Work with AutoTasks: 26.10.2005 (afternoon) OUTLOOK (Short Course I) - E-mail: 2.11.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 2.11.2005 (afternoon) FrontPage 2003 - niveau 1 :  3 - 4.11.2005 (2 jours) AutoCAD 2006 - niveau 1 : 3, 4, 9, 10.11.2005 (4 jours) Joint PVSS JCO...

  16. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: ELEC-2005 Autumn Term - Electronics applications in HEP experiments: 8.11 - 8.12.2005 (10 x 2h lectures) The CERN EDMS for Local Administrators: 15-16.11.2005 (2 days, free of charge) OUTLOOK (Short Course II) - Calendar, Tasks and Notes: 16.11.2005 (morning) Hands-On Object Oriented Design and Programming with C++ : 16 - 18.11.2005 (3 days) The CERN EDMS for Engineers: 17.11.2005 (1 day, free of charge) The Java Programming Language Level 1: 21 - 23.11.2005 (3 days) Hands-on Introduction to Python Programming: 28 - 30.11.2005 (3 days) OUTLOOK (Short Course III) - Meetings and Delegation: 30.11.2005 (morning) WORD 2003 (Short Course III) - HowTo... Work with long documents : 30.11.2005 (afternoon) FrontPage 2003 - niveau 2 : 5 - 6.12.2005 (2 jours) LabVIEW Application Development (Intermediate 1): 5 - 7.12.2005 (3 d...

  17. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: WORD 2003 (Short Course I) - HowTo... Work with AutoTasks (AutoText, AutoFormat, AutoCorrect, Lists, Find/Replace): 25.8.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 25.8.2005 (afternoon) EXCEL 2003 (Short Course I) - HowTo... Work with formulae: 26.8.2005 (morning) WORD 2003 (Short Course III) - HowTo... Work with long documents: 26.8.2005 (afternoon) FrontPage 2003 - niveau 1 : 1 - 2.9.2005 (2 jours) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.9.2005 (1 jour) LabVIEW base 1 : 5 - 7.9.2005 (3 jours) Introduction à Windows XP au CERN : 12.9.2005 (1 demi-journée) FrontPage 2003 - niveau 2 : 15 - 16.9.2005 (2 jours) AutoCAD 2005 - niveau 1 : 22, 23, 28, 29.9.2005 (4 jours) Finite State Machines in the JCOP Framework:  26 - 28.9.2005 (3 days) MAGNE-05 - Magnétisme pour l'électrotechniq...

  18. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    Le nombre de places disponibles peut varier. Veuillez consulter notre site Web pour avoir la dernière mise à jour. Des places sont disponibles dans les cours suivants :   Finite State Machines in the JCOP Framework:  26 - 28.9.2005 (3 days) MAGNE-05 - Magnétisme pour l'électrotechnique : 27 - 29.9.2005 (3 jours) WORD 2003 (Short Course IV) - HowTo... Work with master document: 28.9.2005 (morning) Joint PVSS JCOP Framework : 3 - 7.10.2005 (5 days) Introduction à Dreamweaver MX :  3 - 4.10.2005 (2 jours) Utilisation des fichiers PDF avec Acrobat 7.0 :   4.10.2005 (1 journée) LaTeX par la pratique :   4 - 6.10.2005 (3 matinées) PowerPoint 2003 (F) :  7.10.2005 (1 journée) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) WORD 2003 (Short Course I) - HowTo... Work with AutoTasks: 26.10.2005 (afternoon) OUTLOOK (Short Course I) - E-mail: 2.11.2005 (morning) WORD 2003 (...

  19. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Instructor-led WBTechT Study or Follow-up for Microsoft Applications : 7.9.2004 7.9.2004 Outlook (short course III) : Meetings and Delegation : 7.9.2004 (2 hours, afternoon) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 7 & 8.9.2004 (2 jours) Joint PVSS JCOP Framework : 13 - 17.9.2004 (5 days) Programmation STEP7 niveau 1 : 14-17.9.2004 (4 jours) ANSYS : Thermal Analysis : 22 - 24.9.2004 (3 days) LabVIEW Migration 6 à 7 : 23.9.2004 (one day) ANSYS : Advanced Topics : 27.9 - 1.10.2004 (5 days) Word 2003 - niveau 2 : 27 & 28.9.2004 (2 jours) LabVIEW - Basics 1 : 27 - 29.9.2004 (3 days) MAGNE-04 : Magnétisme pour l'électrotechnique : 28 - 30.9.2004 (3 jours) LabVIEW - Basics 2 : 30.9 & 1.10.2004 (2 days) Introduction à Windows XP au CERN : 4.10.2004 (matin) ...

  20. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Joint PVSS JCOP Framework : 9 - 13.8.2004 (5 days) Outlook (I): E-mail : 31.8.2004 (2 hours, morning) Outlook (II): Calendar, Tasks and Notes : 31.8.2004 (2 hours, afternoon) Hands-on Introduction to Python Programming : 1 - 3.9.2004 (3 days - free course) Instructor-led WBTechT Study or Follow-up for Microsoft Applications : 7.9.2004 (morning) Outlook (III): Meetings and Delegation : 7.9.2004 (2 hours, afternoon) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 7 & 8.9.2004 (2 jours) Joint PVSS JCOP Framework : 13 - 17.9.2004 (5 days) AutoCAD 2002 - niveau 1 : 13, 14, 23, 24.9.2004 (4 jours) Programmation STEP7 niveau 1 : 14 - 17.9.2004 (4 jours) FrontPage 2003 - niveau 1 : 20 & 21.9.2004 (2 jours) ANSYS: Thermal Analysis : 22 - 24.9.2004 (3 days) ANSYS: Advanced Topics : 27.9...

  1. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: LabVIEW - Basics 2 : 30.9 & 1.10.2004 (2 days) Introduction à Windows XP au CERN : 4.10.2004 (matin) The EDMS MTF in practice : 4.10.2004 (afternoon) The CERN EDMS for Engineers : 6.10.2004 (1 day) FrontPage 2003 - niveau 1 : 7 & 8.10.04 (2 jours) Hands-On Object Oriented Design and Programming with C++ : 9 - 12.11.2004 (3 days) FileMaker - niveau 1 : 18 & 19.10.2004 (2 jours) Outlook (short course I) : E-mail : 22.10.2004 (2 hours, morning) Outlook (short course II) : Calendar, Tasks and Notes: 22.10.2004 (2 hours, afternoon) Excel 2003 - niveau 1 : 4 & 5.11.2004 (2 jours) Instructor-led WTechT Study or Follow-up for Microsoft Applications : 9.11.2004 (morning) Outlook (Short Course III) : Meetings and Delegation : 9.11...

  2. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: ANSYS : Thermal Analysis : 22 - 24.9.2004 (3 days) LabVIEW Migration 6 à 7 : 23.9.2004 (one day) ANSYS : Advanced Topics : 27.9 - 1.10.2004 (5 days) Word 2003 - niveau 2 : 27 & 28.9.2004 (2 jours) LabVIEW - Basics 1 : 27 - 29.9.2004 (3 days) LabVIEW - Basics 2 : 30.9 & 1.10.2004 (2 days) Introduction à Windows XP au CERN : 4.10.2004 (matin) The EDMS MTF in practice : 4.10.2004 (afternoon) The CERN EDMS for Engineers : 6.10.2004 (1 day) FrontPage 2003 - niveau 1 : 7 & 8.10.04 (2 jours) Outlook (short course I) : E-mail : 22.10.2004 (2 hours, morning) Outlook (short course II) : Calendar, Tasks and Notes: 22.10.2004 (2 hours, afternoon) Instructor-led WTechT Study or Fo...

  3. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The CERN EDMS for Engineers : 6.10.2004 (1 day) FileMaker - niveau 1 : 18 & 19.10.2004 (2 jours) Outlook (short course I) : E-mail : 22.10.2004 (2 hours, morning) Outlook (short course II) : Calendar, Tasks and Notes: 22.10.2004 (2 hours, afternoon) Excel 2003 - niveau 1 : 4 & 5.11.2004 (2 jours) LabVIEW Intermediate I : 8 - 10.11.2004 (3 days) Instructor-led WTechT Study or Follow-up for Microsoft Applications : 9.11.2004 (morning) Hands-On Object Oriented Design and Programming with C++ : 9 - 11.11.2004 (3 days) Outlook (Short Course III) : Meetings and Delegation : 9.11.2004 (2 hours, afternoon) LabVIEW Intermediate II : 11 & 12.11.2004 (2 days) AutoCAD 2002 - niveau 1 : 11, 12, 18, 19.11.2004 (4 jours) C++ for Particle Physicists : 15 - 19.11.2004 (6 X 3 hours sessions...

  4. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: C++ for Particle Physicists : 8 - 12.3.2004 (6 X 4-hour sessions) Introduction to the CERN EDMS : 9.3.2004 (1 day, free of charge) The EDMS MTF in Practice : 10.3.2004 (morning, free of charge) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon, free of charge) The CERN EDMS for Engineers : 11.3.2004 (1 day, free of charge) LabVIEW...

  5. Technical Training: Places available

    CERN Document Server

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Instructor-led WBTechT study or follow-up for Microsoft applications : 1.4.2004 (morning) FrontPage XP - niveau 1 : 5 & 6.4.2004 (2 jours) AutoCAD 2002 - niveau 1 : 19, 20.4 et 3, 4.5.2004 (4 jours) Oracle 8i/9i - Develop Web-based Applications with PL/SQL : 19 & 20.4.2004 (2 days) Introduction to ANSYS : 20 - 23.4.2004 (4 day...

  6. Technical Training: Places available

    CERN Document Server

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: LabVIEW base 1 : 25 - 27.2.2004(3 jours) Instructor-led WBTechT study or follow-up for Microsoft applications : 26.2.2004 (morning) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon - free of charge) C++ for Particle Physicists : 8 - 12.3.2004 (6 X 4-hour sessions) LabVIEW hands-on (E) : 16.3.2004 (afternoon) LabVIEW Basics 1 : 22 - 24.3.2004 ...

  7. TECHNICAL TRAINING: Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval Tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The EDMS-MTF in practice (free of charge) : 28 -  30.10.03 (6 half-day sessions) AutoCAD 2002 – Level 1 : 3, 4, 12, 13.11.03 (4 days) LabVIEW TestStand ver. 3 : 4 & 5.11.03 (2 days) Introduction to PSpice : 4.11.03 p.m. (half-day) Hands-on Introduction to Python Programming : 12 – 14.11.03 (3 days) ACCESS ...

  8. Technical Training: Places available

    CERN Document Server

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Computational Electromagnetics with the ELEKTRA Module of OPERA-3D : 27 & 28.4.2004 (2 days) Hands-on Introduction to Python Programming : 3 - 5.5.2004 (3 days) LabVIEW Base 2 : 6 & 7.5.2004 (2 jours) Project Planning with MS-Project : 6 & 13.5.2004 (2 days) Word 2003 - niveau 1 : 10 & 11.5.2004 (2 jours) Oracle 9i : SQL : 10 - 12.5.2004 (3...

  9. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: AutoCAD 2002 - niveau 1 : 13, 14, 23, 24.09.2004 (4 jours) Introduction to the CERN EDMS : 22.6.2004 (1 day) The CERN EDMS for local administrators : 23 & 24.6.2004 (2 days) MAGNE-04 : Magnétisme pour l'électrotechnique : 6 - 8.7.2004 (3 jours) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 7 & 8.9.2004 (2 jours) ENSEIGNEMENT TECHNIQUE TECHNICAL TRAINING...

  10. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Joint PVSS JCOP Framework : 9 - 13.8.2004 (5 days) Introduction à Outlook : 19.8.2004 (1 jour) Outlook (Short Course I): E-mail: 31.8.2004 (2 hours, morning) Outlook (Short Course II): Calendar, Tasks and Notes: 31.8.2004 (2 hours, afternoon) Instructor-led WBTechT Study or Follow-up for Microsoft Applications: 7.9.2004 (morning) Outlook (Short Course III): Meetings and Delegation: 7.9.2004 (2 hours, afternoon) I...

  11. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Outlook (short course I) : E-mail : 31.8.2004 (2 hours, morning) Introduction à Outlook : Outlook (short course II) : Calendar, Tasks and Notes : 31.8.2004 (2 hours, afternoon) Instructor-led WBTechT Study or Follow-up for Microsoft Applications : 7.9.2004 (morning) Outlook (short course III) : Meetings and Delegation : 7.9.2004 (2 hours, afternoon) Introduction au VHDL et utilisation du simulateur ...

  12. Technical Training: Places available

    CERN Document Server

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Joint PVSS JCOP Programming : 9 - 13.8.2004 (5 days) Hands-on Introduction to Python Programming: 1 - 3.9.2004 (3 days - free course) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 7 & 8.9.2004 (2 jours) Joint PVSS JCOP Programming : 13 - 17.9.2004 (5 days) AutoCAD 2002 - niveau 1 : 13, 14, 23, 24.9.2004 (4 jours) Programmation STEP7 niveau 1 : 14-17.9.2004 (4...

  13. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Project Planning with MS-Project :6 & 13.5.2004 (2 days) Word 2003 - niveau 1 : 10 & 11.5.2004 (2 jours) Oracle 9i : SQL : 17 - 19.5.2004 (3 days) Word 2003 - niveau 2 : 24 & 25.5.2004 (2 jours) EXCEL 2003 - niveau 1: 27 & 28.5.2004 (2 jours) STEP7 Programming Level 1 : 1 - 4.6.2004 (4 days) Oracle 9i : Programming with PL/SQL : 2 - 4.6.2...

  14. Technical Training: Places available

    CERN Document Server

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Instructor-led WBTechT study for Microsoft applications :12.2.2004 (morning) Instructor-led WBTechT study or follow-up for Microsoft applications : 19.2.2004 (morning) LabVIEW TestStand I (E) : 23 & 24.2.2004 (2 days) LabVIEW base 1 : 25 - 27.2.2004 (3 jours) Instructor-led WBTechT study or follow-up for Microsoft applications : 19.2.2004 (morning) CLEAN-2002 ...

  15. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: AutoCAD 2002 - niveau 1 : 19, 20.4 et 3, 4.5.2004 (4 jours) Oracle 8i/9i - Develop Web-based Applications with PL/SQL : 19 & 20.4.2004 (2 days) Introduction to ANSYS : 20 - 23.4.2004 (4 days) LabVIEW Hands-on : 20.4.2004 (half-day, p.m.) FrontPage XP - niveau 2 : 26 & 27.4.2004 (2 jours) The Joint PVSS JCOP Framework : 26 -...

  16. Technical Training: Places available**

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The JAVA Programming Language Level 1 : 9 & 10.1.2004 (2 days) LabVIEW TestStand I (E) : 23 & 24.2.2004 (2 days) LabVIEW base 1 : 25 - 27.2.2004 (3 jours) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon - free of charge) C++ for Particle Physicists : 8 - 12.3.2004 ( 6 X 4-hour sessions) LabVIEW hands-on (E) 16.3...

  17. Technical Training: Places available

    CERN Document Server

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Instructor-led WBTechT study or follow-up for Microsoft applications : 19.2.2004 (morning) LabVIEW TestStand I (E) : 23 & 24.2.2004 (2 days) LabVIEW base 1 : 25 - 27.2.2004 (3 jours) Instructor-led WBTechT study or follow-up for Microsoft applications : 26.2.2004 (morning) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon - free of charge) C++ for Pa...

  18. Technical Training: Places available

    CERN Document Server

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval - Tel.74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Word 2003 - niveau 2 : 24 & 25.5.2004 (2 jours) VisualEliteHDL : 25 & 26.5.2004 (2 days) EXCEL 2003 - niveau 1 : 27 & 28.5.2004 (2 jours) STEP7 Programming Level 1: 1 - 4.6.2004 (4 days) Oracle 9i : Programming with PL/SQL: 2 - 4.6.2004 (3 days) CST Microwave Studio: 3 & 4.6.2004 (2 days) Oracle 9...

  19. Technical Training: Places Available

    CERN Document Server

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval - Tel.74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Oracle 9i: SQL: 17 - 19.5.2004 (3 days) Word 2003 - niveau 2 : 24 & 25.5.2004 (2 jours) EXCEL 2003 - niveau 1 : 27 & 28.5.2004 (2 jours) STEP7 Programming Level 1: 1 - 4.6.2004 (4 days) Oracle 9i : Programming with PL/SQL: 2 - 4.6.2004 (3 days) CST Microwave Studio: 3 & 4.6.2004 (2 days) Oracle 9i : New f...

  20. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: FrontPage XP - niveau 2 : 26 & 27.4.2004 (2 jours) The Joint PVSS JCOP Framework : 26 - 30.4.2004 (5 days) Computational Electromagnetics with the ELEKTRA Module of OPERA-3D : 27 & 28.4.2004 (2 days) Hands-on Introduction to Python Programming : 3 - 5.5.2004 (3 days) LabVIEW Base 2 : 6 & 7.5.2004 (2 jours) Project Pla...

  1. TECHNICAL TRAINING: Places available**

    CERN Document Server

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Hands-on Introduction to Python Programming : 12 - 14.11.03(3 days) ACCESS 2000 - niveau 1 : 13 & 14.11.03 (2 jours) C++ for Particle Physicists : 17 - 21.11.03 (6 X 3-hour lectures) Programmation automate Schneider TSX Premium - niveau 2 : 18 - 21.11.03 (4 jours) Project Planning with MS-Project  (free of charg...

  2. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Hands-on Introduction to Python Programming: 1 - 3.9.2004 (3 days - free course) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 7 & 8.9.2004 (2 jours) AutoCAD 2002 - niveau 1 : 13, 14, 23, 24.9.2004 (4 jours) Programmation STEP7 niveau 1 : 14-17.9.2004 (4 jours) FrontPage 2003 - niveau 1 : 20 & 21.9.2004 (2 jours) Word 2003 - niveau 2 : 27 & 28.9.2004 (2 jours) Introduction à Wind...

  3. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: C++ for Particle Physicists : 8 - 12.3.2004 (6 X 4-hour sessions) Introduction to the CERN EDMS : 9.3.2004 (1 day, free of charge) The EDMS MTF in Practice : 10.3.2004 (morning, free of charge) CLEAN-2002: Working in a Cleanroom : 10.3.2004 (afternoon, free of charge) The CERN EDMS for Engineers : 11.3.2004 (1 day, free of charge) LabVIEW hands-on (E):...

  4. Technical Training: Places available

    CERN Document Server

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Oracle 9i : New features for developers : 14 - 16.6.2004 (3 days) The Joint PVSS JCOP Framework: 14 - 18.6.2004 (5 days) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 15 & 16.6.2004 (2 jours) EXCEL 2003 - niveau 2 : 17 & 18.6.2004 (2 jours) MAGNE-04 : Magnétisme pour l'électrotechnique : 6 au 8.7.2004 (3 jours) AutoCAD 2002 - niveau 1 : 13, 14, 23, 24.09.2004 (4 jours) ENSEIGNEMEN...

  5. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Introduction to the CERN EDMS : 22.6.2004 (1 day) The CERN EDMS for local administrators : 23 & 24.6.2004 (2 days) Compatibilité électromagnétique (CEM) - Introduction / Electromagnetic Compatibility (EMC) - Introduction: 7.7.2004 (morning) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 7 & 8.9.2004 (2 jours) AutoCAD 2002 - niveau 1 : 13, 14, 23, 24.9.2004 (4 jours) Programmation STEP...

  6. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Joint PVSS JCOP Framework : 9 - 13.8.2004 (5 days) Introduction à Outlook : 19.8.2004 (1 jour) Outlook (Short Course I): E-mail: 31.8.2004 (2 hours, morning) Outlook (Short Course II): Calendar, Tasks and Notes: 31.8.2004 (2 hours, afternoon) Hands-on Introduction to Python Programming: 1 - 3.9.2004 (3 days - free course) Instructor-led WBTechT Study or Follow-up for Microsoft Applications: 7.9.20...

  7. TECHNICAL TRAINING: PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: LabVIEW Basics 1 : 22 - 24.3.2004 (3 days) Oracle 9i : New Features for Developers : 22 - 24.3.2004 (3 days) Instructor-led WBTechT study or follow-up for Microsoft applications : 1.4.2004 (morning) FrontPage XP - niveau 1 : 5 & 6.4.2004 (2 jours) AutoCAD 2002 - niveau 1 : 19, 20.4 et 3, 4.5.2004 (4 jours) Oracle 8i/9i - Develop Web-based Applications with PL/SQL : 19 & 20.4.2004 (2 days) Introduction to ANSYS : 20 - 23.4.2004 (4 days) LabVIEW hands-on (E) : 20.4.2004 (afternoon, free of charge) FrontPage XP - niveau 2 : 26 & 27.4.2004 (2 jours) LabVIEW Base 2 : 6 & 7.5.2004 (2 jours) Word XP - niveau 1 : 10 & 11.5.2004 (2 jours) Word XP - niveau 2 : 24 & 25.5.2004 (2 jours) If you wish to participate in one of these courses, pleas...

  8. THE PLACES FOR PARTICIPATION

    Directory of Open Access Journals (Sweden)

    Tania Carson

    2007-09-01

    Full Text Available The current upsurge of interest in street art with its direct approach to the city emphasizes the need to readdress public arts’ role within the wider social and cultural context. As it becomes routine to incorporate art works into city planning and city life, art has become both ‘safe’ and ‘user‐friendly’. On the one hand, art is subsumed and immersed into the wider culture through leisure and entertainment policies, socially engaged public art projects, community art and urban design. On the other hand, there are those works which deliberately intend to unsettle or confront the viewer. In recent years the term intervention has been ascribed to unorthodox art works in the public sphere which are often illicit, or at least non‐complicit in the prevailing hegemony. These practices are disruptive due to their enactment outside traditional art spaces, often in the streets. By occupying the streets, places of democracy and disjunction, these interventions transform public space back into a place of overt cultural and political expression. They also deliberately involve the inhabitants of the city by utilizing everyday spaces in ways which offer an unmediated sense of the artwork.

  9. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: LabVIEW base 1/basics 1 : 31.1 - 2.2.2005 (3 jours/3 days - langue à décider/language to be decided) LabVIEW base 2/basics 2 : 3 & 4.2.2005 (2 jours/2 days - langue à décider/language to be decided) Finite State Machines in the JCOP Framework: 1 - 3.2.2005 (3 days - free course) The JAVA Programming Language Level 2: 7 - 9.2.2005 (3 days) C++ Programming Advanced -Traps and Pitfalls: 8 - 11.2.2005 (4 days) Joint PVSS JCOP Framework: 14 - 18.2.2005 (5 days) JAVA 2 Enterprise Edition - Part 1: WEB Applications: 21 & 22.2.2005 (2 days) FrontPage 2003 - niveau 1 : 21 & 22.2.2005 (2 jours) JAVA 2 Enterprise Edition - Part 2: Enterprise JavaBeans: 23 - 25.2.2005 (3 days) ELEC-2005 - Spring Term: Integrated circuits and VLSI technology...

  10. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: The CERN EDMS for Local Administrators: 19 & 20.1.2005 (2 days - free course) Programmation Unity-Pro pour utilisateurs de Schneider PL7-Pro : 24 - 28.1.2005 (9 demi-journées) LabVIEW base 1/basics 1 : 31.1 - 2.2.2005 (3 jours/3 days - langue à décider/language to be decided) LabVIEW base 2/basics 2 : 3 & 4.2.2005 (2 jours/2 days - langue à décider/language to be decided) Finite State Machines in the JCOP Framework: 1 - 3.2.2005 (3 days - free course) The JAVA Programming Language Level 2: 7 - 9.2.2005 (3 days) C++ Programming Advanced -Traps and Pitfalls: 8 - 11.2.2005 (4 days) Joint PVSS JCOP Framework: 14 - 18.2.2005 (5 days) JAVA 2 Enterprise Edition - Part 1: WEB Applications: 21 & 22.2.2005 (2 days) JAVA 2 Enterprise Edition - Part 2: Enterprise JavaBeans: 23 - 25.2.2005 (3 days) ELEC-2005 â...

  11. Technical training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: LabVIEW Intermediate I : 8 - 10.11.2004 (3 days) Instructor-led WTechT Study or Follow-up for Microsoft Applications : 9.11.2004 (morning) Outlook (Short Course III) : Meetings and Delegation : 9.11.2004 (2 hours, afternoon) LabVIEW Intermediate II : 11 & 12.11.2004 (2 days) AutoCAD 2002 - niveau 1 : 11, 12, 18, 19.11.2004 (4 jours) C++ for Particle Physicists : 15 - 19.11.2004 (6 X 3 hours sessions) Word 2003 - niveau 1 : 22 & 23.11.2004 (2 jours) Introduction à ANSYS : 23 - 26.11.2004 (4 jours) Project Planning with MS-Project : 25.11 & 2.12.2004 (2 days) Introduction to PERL 5 : 8 & 9.12.2004 (2 days) Advanced aspects of PERL 5 : 10.12.2004 (1 day) The JAVA Programming Language Level 1 : 11 & 12.1.2005 (2 days) Introduction...

  12. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Outlook (short course I) : E-mail : 22.10.2004 (2 hours, morning) Outlook (short course II) : Calendar, Tasks and Notes: 22.10.2004 (2 hours, afternoon) Excel 2003 - niveau 1 : 4 & 5.11.2004 (2 jours) LabVIEW Intermediate I : 8 - 10.11.2004 (3 days) Instructor-led WTechT Study or Follow-up for Microsoft Applications : 9.11.2004 (morning) Hands-On Object Oriented Design and Programming with C++ : 9 - 11.11.2004 (3 days) Outlook (Short Course III) : Meetings and Delegation : 9.11.2004 (2 hours, afternoon) LabVIEW Intermediate II : 11 & 12.11.2004 (2 days) AutoCAD 2002 - niveau 1 : 11, 12, 18, 19.11.2004 (4 jours) C++ for Particle Physicists : 15 - 19.11.2004 (6 X 3 hours sessions) FrontPage 2003 - niveau 2 : 18 & 19.11.2004 (2 jours) Word 2003 - niveau 1 : 22 &a...

  13. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: LabVIEW Intermediate I : 8 - 10.11.2004 (3 days) Instructor-led WTechT Study or Follow-up for Microsoft Applications : 9.11.2004 (morning) Hands-On Object Oriented Design and Programming with C++ : 9 - 11.11.2004 (3 days) Outlook (Short Course III) : Meetings and Delegation : 9.11.2004 (2 hours, afternoon) LabVIEW Intermediate II : 11 & 12.11.2004 (2 days) AutoCAD 2002 - niveau 1 : 11, 12, 18, 19.11.2004 (4 jours) C++ for Particle Physicists : 15 - 19.11.2004 (6 X 3 hours sessions) Word 2003 - niveau 1 : 22 & 23.11.2004 (2 jours) Introduction à ANSYS : 23 - 26.11.2004 (4 jours) CLEAN-2002 - Travailler en salle propre : 23.11.2004 (après-midi, cours gratuit) Project Planning with MS-Project : 25.11 & 2.12.2004 (2 days) PCAD Sch&eac...

  14. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: FileMaker - niveau 1 : 18 & 19.10.2004 (2 jours) Outlook (short course I) : E-mail : 22.10.2004 (2 hours, morning) Outlook (short course II) : Calendar, Tasks and Notes: 22.10.2004 (2 hours, afternoon) Excel 2003 - niveau 1 : 4 & 5.11.2004 (2 jours) LabVIEW Intermediate I : 8 - 10.11.2004 (3 days) Instructor-led WTechT Study or Follow-up for Microsoft Applications : 9.11.2004 (morning) Hands-On Object Oriented Design and Programming with C++ : 9 - 11.11.2004 (3 days) Outlook (Short Course III) : Meetings and Delegation : 9.11.2004 (2 hours, afternoon) LabVIEW Intermediate II : 11 & 12.11.2004 (2 days) AutoCAD 2002 - niveau 1 : 11, 12, 18, 19.11.2004 (4 jours) C++ for Particle Physicists : 15 - 19.11.2004 (6 X 3 hours sessions) FrontPage 2003 - niveau 2 : 18 &a...

  15. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: LabVIEW Intermediate I : 8 - 10.11.2004 (3 days) Instructor-led WTechT Study or Follow-up for Microsoft Applications : 9.11.2004 (morning) Hands-On Object Oriented Design and Programming with C++ : 9 - 11.11.2004 (3 days) Outlook (Short Course III) : Meetings and Delegation : 9.11.2004 (2 hours, afternoon) LabVIEW Intermediate II : 11 & 12.11.2004 (2 days) AutoCAD 2002 - niveau 1 : 11, 12, 18, 19.11.2004 (4 jours) C++ for Particle Physicists : 15 - 19.11.2004 (6 X 3 hours sessions) Word 2003 - niveau 1 : 22 & 23.11.2004 (2 jours) Introduction à ANSYS : 23 - 26.11.2004 (4 jours) CLEAN-2002 - Travailler en salle propre : 23.11.2004 (après-midi, cours gratuit) Project Planning with MS-Project : 25.11 & 2.12.2004 (2 days) ENSEIGNEMENT ...

  16. In-place HEPA filter penetration test

    International Nuclear Information System (INIS)

    Bergman, W.; Wilson, K.; Elliott, J.; Bettencourt, B.; Slawski, J.W.

    1997-01-01

    We have demonstrated the feasibility of conducting penetration tests on high efficiency particulate air (HEPA) filters as installed in nuclear ventilation systems. The in-place penetration test, which is designed to yield equivalent penetration measurements as the standard DOP efficiency test, is based on measuring the aerosol penetration of the filter installation as a function of particle size using a portable laser particle counter. This in-place penetration test is compared to the current in-place leak test using light scattering photometers for single HEPA filter installations and for HEPA filter plenums using the shroud method. Test results show the in-place penetration test is more sensitive than the in-place leak test, has a similar operating procedure, but takes longer to conduct. Additional tests are required to confirm that the in-place penetration test yields identical results as the standard dioctyl phthalate (DOP) penetration test for HEPA filters with controlled leaks in the filter and gasket and duct by-pass leaks. Further development of the procedure is also required to reduce the test time before the in- place penetration test is practical

  17. Esporulação de Pyricularia grisea em diferentes meios de cultura e regimes de luz Sporulation of Pyricularia grisea on different culture media and light regimes

    Directory of Open Access Journals (Sweden)

    Maria Fernanda Antunes da Cruz

    2009-08-01

    Full Text Available Diferentes fatores influenciam o crescimento e a esporulação dos fungos, tais como: composição do meio de cultivo, temperatura e luz. O objetivo do trabalho foi determinar o melhor meio de cultivo e regime de luz para a esporulação de Pyricularia grisea. Um isolado deste, obtido da cultivar de trigo 'BRS 194', foi repicado por disco micelial em oito meios de cultivo (farinha integral de arroz, aveia, centeio, cevada, trigo, triticale; BSA e extrato de tomate e submetido a três regimes de luz (escuro, fotoperíodo de 12h e luz contínua. As placas permaneceram por 10 dias, em câmara de crescimento sob temperatura de 23-25°C. Em seguida, o número de conídios foi determinado em câmara de Neubauer. O meio de cultivo à base de farinha integral de trigo propiciou a maior esporulação do fungo em todos os regimes de luz, e a maior esporulação ocorreu em regime de luz contínua para todos os meios de cultivos testados. Os meios de arroz e BSA propiciaram uma menor produção de conídios pelo fungo.A set of conditions is necessary for the growth and sporulation of fungi, particularly the composition of culture media, temperature and light. The objective of this research was to determine the ideal culture medium and light regime for the sporulation of P. grisea. Micelial discs of an isolate, obtained from cultivar 'BRS 194' of wheat, were transferred to eight culture media (rice wholemeal, oat, rye, barley, wheat, triticale, PSA and tomato sauce and kept in the dark, under continuous light and 12h of light. In a growing chamber, for 10 days at 23-25°C. Then, the number of conidia was determined in a Neubauer chamber. The higher sporulation, regardless of light regime, was observed on wheat wholemeal culture medium. The continuous light provided higher sporulation this fungus in all culture medium. The lowest production of conidia was observed on rice wholemeal and PSA culture media.

  18. Take the "C" Train

    Science.gov (United States)

    Lawton, Rebecca

    2008-01-01

    In this essay, the author recalls several of her experiences in which she successfully pulled her boats out of river holes by throwing herself to the water as a sea-anchor. She learned this trick from her senior guides at a spring training. Her guides told her, "When you're stuck in a hole, take the "C" train."" "Meaning?" The author asked her…

  19. Social Perspective Taking

    Science.gov (United States)

    2009-09-01

    having testicular cancer , they can and do attempt the perspective taking leap (Halpern, 2001). Individuals can strive to know themselves better by...science, social science, psychology , and anthropological literature in the areas of education, medicine, and cross-cultural interactions. 2. Identify...experts in the fields of social psychology , intercultural research, and medicine in order to determine the critical KSAs to be included in a training

  20. Healthy Places for Healthy People

    Science.gov (United States)

    Describes the Healthy Places for Healthy People technical assistance program that helps communities create walkable, healthy, economically vibrant places by engaging with local health care facility partners

  1. Strangers in Familiar Places

    DEFF Research Database (Denmark)

    Muhr, Sara Louise

    2012-01-01

    Employees working across multiple cultures are exposed to a vast number of different norms and values, and consequentially work is often a struggle to retain a coherent sense of self. However, when international workers travel, they also encounter more bland spaces where familiarity and similarity...... are important. These spaces appear culturally generic to the Western traveler, but are highly Westernized to bring comfort to Western employees traveling in foreign cultures. This paper argues that these spaces are important in cross-cultural identity work in the sense that international workers – professional...... strangers – need these places to belong and relate to familiarity and to regain a sense of identity. Drawing on an illustrative empirical vignette of an international consultant, I demonstrate how culturally generic spaces can be used in identity work of an international relations consultant....

  2. Pushing Personhood into Place

    DEFF Research Database (Denmark)

    Bidwell, Nicola J.; Winschiers-Theophilus, Heike; Kapuire, Gereon K.

    2011-01-01

    - recorded video and participants' interpretations and interactions with thumbnail photos from video, photography and writing materials. These themes use some classical anthropological 'tropes' and describe verbal and bodily interactions, relationships between bodies, artifacts and settings and concepts...... for generations; but technology-use can inadvertently displace knowledge for communities with knowledge traditions that differ from those of technology-design. Devices which are sensitive to users' locations, combined with platforms for social networking and user-generated-content, offer intriguing opportunities...... about personhood, community and place. We adopt a critical attitude towards assumptions about spatial, temporal and social logics and literacies to promote a design sensitivity to local experiences of locations. This motivates us to emphasize connections, not points, and the bodily absorption...

  3. Technical Training - Places available

    CERN Multimedia

    Monique Duval

    2003-01-01

    Places are available in the following courses: HeREF-2003 : Techniques de la réfrigération Hélium :  6 - 10.10.2003  (7 demi-journées, cours en français avec support en anglais)  The Java Programming Language Level 1:  6 - 7.10.2003  (2 days) Java 2 Enterprise Edition - Part 2: Enterprise JavaBeans: 8 - 10.10.2003  (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 :  20 & 22.10.03  (2 jours) AutoCAD 2002 - niveau 1 :  20, 21, 27, 28.10.03  (4 jours) CLEAN-2002 : Working in a Cleanroom: 23.10.03  (half day, free of charge) Plannification de projet avec MS-Project/ Project Scheduling with MS-Project : 2 sessions:  23.10 & 4.11.03  (2 jours/2 days and 18 &25.11.03 langue à définir/language to be defined) AutoCAD 2002 - Level 1:  3, 4, 12, 13.11.03  (4 days) Introduction to Pspice:  4.11.03p.m.  (half-day) AutoCAD 2002 - niveau 2 :  10 & 11.11.03  (2 jours) ACCESS 2000 - niveau 1 :  13 & 14.11.03  (2 jours) A...

  4. Influence of the Oil Pequi in the Growth Mycelial and Sporulation of Colletotrichum Musae and Growth Micelial Of Rhizopus sp. in vitro.

    Directory of Open Access Journals (Sweden)

    B. L. Mendes

    2013-07-01

    Full Text Available Abstract: The anthracnose caused by Colletotrichum musae and soft rot caused by Rhizopus sp are post-harvest diseases that influenced quality of exported fruits. To control these diseases fungicides are used, but in light of these waste products in the fruit and the environment, alternative control methods such as using biofungicides have been studied. The objective of this study was to evaluate the effect of pequi oil on mycelia growth and sporulation of Colletotrichum musae and mycelia growth of Rhizopus sp. in vitro. The oil was added in aliquots of 5, 10, 15, 20, 25, and 50L in the center of Petri dishes containing approximately 15mL of Potato-Dextrose-ar (PDA and distributed over the surface of the medium with Drigalskys handle. After 24 hours, a disc of 8mm diameter from mycelium of fungi was peaked to the center of the plates. As a control, were used Petri dishes containing only PDA. Five replicates were used for each aliquot. After analysis, it was concluded that the pequi oil is ineffective for controlling these pathogens because induced mycelium growth and sporulation.Key Words: Phytopathogens, alternative control, anthracnose, soft rot

  5. Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis.

    Science.gov (United States)

    Webb, C D; Decatur, A; Teleman, A; Losick, R

    1995-10-01

    We report the use of the green fluorescent protein (GFP) of Aequorea victoria to visualize cell-specific gene expression and protein subcellular localization during sporulation in Bacillus subtilis. Sporangia bearing the gene (gfp) for the green fluorescent protein fused to genes under the control of the sporulation transcription factor sigma F exhibited a forespore-specific pattern of fluorescence. Forespore-specific fluorescence could be detected with fusions to promoters that are utilized with low (csfB) and high (sspE-2G) efficiency by sigma F-containing RNA polymerase. Conversely, a mother cell-specific pattern of fluorescence was observed in sporangia bearing a transcriptional fusion of gfp to a spore coat protein gene (cotE) under the control of sigma E and an in-frame fusion to a regulatory gene (gerE) under the control of sigma K. An in-frame fusion of gfp to cotE demonstrated that GFP can also be used to visualize protein subcellular localization. In sporangia producing the CotE-GFP fusion protein, fluorescence was found to localize around the developing spore, and this localization was dependent upon SpoIVA, a morphogenetic protein known to determine proper localization of CotE.

  6. Genome-wide expression profiling, in vivo DNA binding analysis, and probabilistic motif prediction reveal novel Abf1 target genes during fermentation, respiration, and sporulation in yeast.

    Science.gov (United States)

    Schlecht, Ulrich; Erb, Ionas; Demougin, Philippe; Robine, Nicolas; Borde, Valérie; van Nimwegen, Erik; Nicolas, Alain; Primig, Michael

    2008-05-01

    The autonomously replicating sequence binding factor 1 (Abf1) was initially identified as an essential DNA replication factor and later shown to be a component of the regulatory network controlling mitotic and meiotic cell cycle progression in budding yeast. The protein is thought to exert its functions via specific interaction with its target site as part of distinct protein complexes, but its roles during mitotic growth and meiotic development are only partially understood. Here, we report a comprehensive approach aiming at the identification of direct Abf1-target genes expressed during fermentation, respiration, and sporulation. Computational prediction of the protein's target sites was integrated with a genome-wide DNA binding assay in growing and sporulating cells. The resulting data were combined with the output of expression profiling studies using wild-type versus temperature-sensitive alleles. This work identified 434 protein-coding loci as being transcriptionally dependent on Abf1. More than 60% of their putative promoter regions contained a computationally predicted Abf1 binding site and/or were bound by Abf1 in vivo, identifying them as direct targets. The present study revealed numerous loci previously unknown to be under Abf1 control, and it yielded evidence for the protein's variable DNA binding pattern during mitotic growth and meiotic development.

  7. Using Critical Path Analysis (CPA) in Place Marketing process

    OpenAIRE

    Metaxas, Theodore; Deffner, Alex

    2013-01-01

    The article awards the use of CPA as a methodological tool in Place Marketing implementation. Taking into account that Place Marketing is a strategic process based on ‘project’ meaning with particular actions in specific time horizon, the article proposed that CPΑ has the capacity to satisfy this hypothesis. For this reason, the article creates a hypothetical scenario of CPA in four phases, planning, programming, implementation and feedback, taking as a case study the city of Rostock in Germa...

  8. Taking action against violence.

    Science.gov (United States)

    Kunz, K

    1996-05-01

    Significant increase in violent crimes in recent years forced Icelandic men to take action against violence. Television was seen as a major contributory factor in increasing violence. Surveys indicate that 10-15 years after television broadcasting commences in a particular society, the incidence of crime can be expected to double. While the majority of the individuals arrested for violent crimes are men, being male does not necessarily mean being violent. The Men's Committee of the Icelandic Equal Rights Council initiated a week-long information and education campaign under the theme "Men Against Violence". This campaign involved several events including an art exhibit, speeches on violence in families, treatment sought by those who are likely to resort to violence, booklet distribution among students in secondary schools, and a mass media campaign to raise public awareness on this pressing problem.

  9. Taking Design Games Seriously

    DEFF Research Database (Denmark)

    Eriksen, Mette Agger; Brandt, Eva; Mattelmäki, Tuuli

    2014-01-01

    Using design games at Participatory Design (PD) events is well acknowledged as a fruitful way of staging participation. As PD researchers, we have many such experiences, and we have argued that design games connect participants and promote equalizing power relations. However, in this paper, we...... will (self) critically re-connect and reflect on how people (humans) and materials (non-humans) continually participate and intertwine in various power relations in design game situations. The analysis is of detailed situated actions with one of our recent games, UrbanTransition. Core concepts mainly from...... Bruno Latour’s work on Actor-Network-Theory are applied. The aim is to take design games seriously by e.g. exploring how assemblages of humans and non-humans are intertwined in tacitly-but-tactically staging participation, and opening up for or hindering negotiations and decision-making, thus starting...

  10. Taking a Long View

    DEFF Research Database (Denmark)

    Ougaard, Morten

    The purpose of this paper is to discuss capitalism’s long-term prospects. To some extent I draw on material and arguments I have presented elsewhere (Ougaard, 2014; Ougaard, 2015a; Ougaard, 2015b); the present intention is to bring these elements together and combine them with other material...... to elaborate an argument about capitalism’s long term devel-opmental potential. On some points the argument resembles recent gloomy diagnoses for-warded by Wolfgang Streeck (Streeck, 2014a; Streeck, 2014b) and Andrew Gamble (Gamble, 2014), but is also offers an alternative to their interpretations by taking...... factors (CFs). The next step is, drawing on regulation theory, to relate the mobilization of such CFs to the successive stages of capitalist development after World War Two, up to the present crisis, and after this, in the final two sections I discuss the potential for mobilizing ‘surplus labour...

  11. Place attachment, place identity and aesthetic appraisal of urban landscape

    Directory of Open Access Journals (Sweden)

    Jaśkiewicz Michał

    2015-12-01

    Full Text Available As the aesthetic of the Polish cities became a topic of wider discussions, it is important to detect the potential role of human-place relations. Two studies (N = 185 & N = 196 were conducted to explore the relationship between place attachment, place identity and appraisal of urban landscape. Satisfaction with urban aesthetic was predicted by two dimensions of place attachment (place inherited and place discovered, local identity (on the trend level and national-conservative identity. Place discovered and European identity were also predictors of visual pollution sensitivity. Place discovered is considered as more active type of attachment that permits both a positive bias concerning the aesthetics of one’s city, and a stronger criticism of the elements that can potentially violate the place’s landscape.

  12. Introduction to Interregional Place Branding

    DEFF Research Database (Denmark)

    Zenker, Sebastian; Jacobsen, Björn P.

    2015-01-01

    Place branding is an inherently difficult venture, since places are complex systems of geographical abstractions, each one understood in relation and contrast to other geographical entities. Even at the smallest size – a district, town, or city – a place is quite complex, but it becomes even more...... with a high degree of complexity. In this chapter, we will define the place brand and examine different concepts to understand branding as they relate to places. In a next step, we will highlight the special character of interregional place branding. Finally, we will apply these concepts to a particular...

  13. Hijacking culture : The disconnection between place culture and place brands

    NARCIS (Netherlands)

    Kavaratzis, Mihalis; Ashworth, Gregory

    2015-01-01

    This article argues that the dominant understanding of culture within place branding is inadequate and leads to disconnection between local culture and the place brand. The article discusses significant tensions arising from an evident oversimplification of the relationship between place brands and

  14. Place branding, embeddedness and endogenous rural development : Four European cases

    NARCIS (Netherlands)

    Donner, Mechthild; Horlings, Lummina; Fort, Fatiha; Vellema, Sietze

    2017-01-01

    This article deals with place branding on the regional scale, in the rural context of food and tourism networks in Europe. Place branding is linked to the concepts of endogenous rural development, territory and embeddedness, by analysing how the valorisation of specific rural assets takes shape. The

  15. Decision taking as a service

    NARCIS (Netherlands)

    Bergstra, J.A.

    2012-01-01

    Decision taking can be performed as a service to other parties and it is amenable to outtasking rather than to outsourcing. Outtasking decision taking is compatible with selfsourcing of decision making activities carried out in preparation of decision taking. Decision taking as a service (DTaaS) is

  16. Taking the plunge

    CERN Multimedia

    2008-01-01

    On 8 and 15 October, 58 people took the plunge and headed to the Varembé swimming pool in Geneva for their first taste of scuba diving at one of the CERN scuba club’s free trial dives. Club president Alberto Pace, left, taking a new recruit on his first dive. The CERN scuba club was making waves down at the Varembe swimming pool on Wednesday 15 October. Thirty-six people turned up to the club’s second free trial dive. "It was fantastic," said Jörg, one of the new recruits, after his first ever dive. "I’ve always wanted to try diving and this was a free lesson, so I thought I would come and have a go." Fourteen of the club’s fully qualified instructors were there to give one-on-one tuition. After a first dive in the normal pool the new divers moved into the deep pool. Some took to the water like fish, and at one point an impromptu game of aqua-Frisbee broke out, five metres below the surface. Richard Catherall, who organi...

  17. Sense of place and place identity: review of neuroscientific evidence.

    Science.gov (United States)

    Lengen, Charis; Kistemann, Thomas

    2012-09-01

    The aim of this review is to bring the phenomenological sense of place approach together with current results from neuroscience. We searched in neuroscientific literature for ten dimensions which were beforehand identified to be important in a phenomenological sense of place/place identity model: behaviour, body, emotion, attention, perception, memory, orientation, spirituality, meaning/value and culture/sociality. Neuroscience has identified many neurobiological correlates of phenomenological observations concerning sense of place. The human brain comprises specific and specialised structures and processes to perceive, memorise, link, assess and use spatial information. Specific parts (hippocampus, entorhinal, parahippocampal and parietal cortex), subregions (parahippocampal place area, lingual landmark area), and cells (place cells, grid cells, border cells, head direction cells) have been identified, their specific function could be understood and their interaction traced. Neuroscience has provided evidence that place constitutes a distinct dimension in neuronal processing. This reinforces the phenomenological argumentation of human geography and environmental psychology. Copyright © 2012 Elsevier Ltd. All rights reserved.

  18. Why It Takes Prevention, Not Detection, to Fight Bioterrorism

    Science.gov (United States)

    Janata, Jiri (Art)

    2005-01-01

    Following the events which took place on September 11, 2001, and the anthrax attacks which occurred after that date, US authorities became concerned with the idea that an assault with chemical or biological weapons could take place on American territory or in American ships or planes. A worrisome model for such an assault was the 1995 terrorist…

  19. Dual Regulation of Bacillus subtilis kinB Gene Encoding a Sporulation Trigger by SinR through Transcription Repression and Positive Stringent Transcription Control

    Directory of Open Access Journals (Sweden)

    Yasutaro Fujita

    2017-12-01

    Full Text Available It is known that transcription of kinB encoding a trigger for Bacillus subtilis sporulation is under repression by SinR, a master repressor of biofilm formation, and under positive stringent transcription control depending on the adenine species at the transcription initiation nucleotide (nt. Deletion and base substitution analyses of the kinB promoter (PkinB region using lacZ fusions indicated that either a 5-nt deletion (Δ5, nt -61/-57, +1 is the transcription initiation nt or the substitution of G at nt -45 with A (G-45A relieved kinB repression. Thus, we found a pair of SinR-binding consensus sequences (GTTCTYT; Y is T or C in an inverted orientation (SinR-1 between nt -57/-42, which is most likely a SinR-binding site for kinB repression. This relief from SinR repression likely requires SinI, an antagonist of SinR. Surprisingly, we found that SinR is essential for positive stringent transcription control of PkinB. Electrophoretic mobility shift assay (EMSA analysis indicated that SinR bound not only to SinR-1 but also to SinR-2 (nt -29/-8 consisting of another pair of SinR consensus sequences in a tandem repeat arrangement; the two sequences partially overlap the ‘-35’ and ‘-10’ regions of PkinB. Introduction of base substitutions (T-27C C-26T in the upstream consensus sequence of SinR-2 affected positive stringent transcription control of PkinB, suggesting that SinR binding to SinR-2 likely causes this positive control. EMSA also implied that RNA polymerase and SinR are possibly bound together to SinR-2 to form a transcription initiation complex for kinB transcription. Thus, it was suggested in this work that derepression of kinB from SinR repression by SinI induced by Spo0A∼P and occurrence of SinR-dependent positive stringent transcription control of kinB might induce effective sporulation cooperatively, implying an intimate interplay by stringent response, sporulation, and biofilm formation.

  20. 36 CFR 242.27 - Subsistence taking of fish.

    Science.gov (United States)

    2010-07-01

    ... of the pot. The twine may be knotted only at each end and at the attachment points on the galvanic..., species, or place of taking if necessary for conservation purposes); (iii) Each person who takes fish... the previous 5 years. A qualifying program must have instructors, enrolled students, minimum...

  1. Reframing place promotion, place marketing, and place branding - moving beyond conceptual confusion

    NARCIS (Netherlands)

    Boisen, Martin; Terlouw, Kees; Groote, Peter; Couwenberg, Oscar

    2017-01-01

    The literature and practice of place promotion, place marketing and place branding lack a common understanding of what these three concepts mean and through what kind of policies they can be implemented. Although scholars have provided several theoretical frameworks and definitions, both scholars

  2. Antifungal effect of gaseous nitric oxide on mycelium growth, sporulation and spore germination of the postharvest horticulture pathogens, Aspergillus niger, Monilinia fructicola and Penicillium italicum.

    Science.gov (United States)

    Lazar, E E; Wills, R B H; Ho, B T; Harris, A M; Spohr, L J

    2008-06-01

    To evaluate the antifungal activity of nitric oxide (NO) against the growth of the postharvest horticulture pathogens Aspergillus niger, Monilinia fructicola and Penicillium italicum under in vitro conditions. Different volumes of NO gas were injected into the Petri dish headspace to obtain the desired concentrations of 50-500 microl l(-1). The growth of the fungi was measured for 8 days of incubation in air at 25 degrees C. All concentrations of NO were found to produce an antifungal effect on spore germination, sporulation and mycelial growth of the three fungi, with the most effective concentration for A. niger and P. italicum being 100 and 500 microl l(-1) for M. fructicola. Short-term exposure to a low concentration of NO gas was able to inhibit the subsequent growth of A. niger, M. fructicola and P. italicum. NO gas has potential use as a natural fungicide to inhibit microbial growth on postharvest fruit and vegetables.

  3. Effect of sporulation medium and its divalent cation content on the heat and high pressure resistance of Clostridium botulinum type E spores.

    Science.gov (United States)

    Lenz, Christian A; Vogel, Rudi F

    2014-12-01

    Clostridium (C.) botulinum type E belongs to the non-proteolytic physiological C. botulinum group II and produces the highly potent Botulinum neurotoxin E (BoNT/E) even at refrigerated temperatures. As C. botulinum type E spores are highly prevalent in aquatic environments, seafood and fishery products are commonly associated with this organism. Hydrostatic high pressure (HHP) treatments, or treatments combining HHP with elevated temperatures (HHPT), can be used to improve traditional preservation methods and increase food safety, quality and durability. In this study, we assessed the effect of different sporulation media and cation concentration on the heat resistance, HHP resistance, and HHPT resistance of spores from three C. botulinum type E strains. SFE (sediment fish extract) sporulation media yielded the most resistant spores, whereas, in M140 media, the least resistant spores were produced. Furthermore our results indicate that the divalent cation content (Ca(2+), Mg(2+) and Mn(2+)) plays a role in the differential development of C. botulinum type E spore resistance to heat, HHP and HHPT in different media. Calcium cations confer heat and HPPT resistance to spores, while high amounts of magnesium cations appear to have a negative effect. Manganese cations in low concentrations are important for the development resistance to HPP and HPPT treatments, but not heat alone. This study provides valuable information on the nature of non-proteolytic C. botulinum type E spores grown in different media. The data provided here can be useful to the food industry and to researchers when considering spore properties in food safety risk assessment and the experimental design of future inactivation studies. Copyright © 2014 Elsevier Ltd. All rights reserved.

  4. Repression of Middle Sporulation Genes in Saccharomyces cerevisiae by the Sum1-Rfm1-Hst1 Complex Is Maintained by Set1 and H3K4 Methylation.

    Science.gov (United States)

    Jaiswal, Deepika; Jezek, Meagan; Quijote, Jeremiah; Lum, Joanna; Choi, Grace; Kulkarni, Rushmie; Park, DoHwan; Green, Erin M

    2017-12-04

    The conserved yeast histone methyltransferase Set1 targets H3 lysine 4 (H3K4) for mono, di, and trimethylation and is linked to active transcription due to the euchromatic distribution of these methyl marks and the recruitment of Set1 during transcription. However, loss of Set1 results in increased expression of multiple classes of genes, including genes adjacent to telomeres and middle sporulation genes, which are repressed under normal growth conditions because they function in meiotic progression and spore formation. The mechanisms underlying Set1-mediated gene repression are varied, and still unclear in some cases, although repression has been linked to both direct and indirect action of Set1, associated with noncoding transcription, and is often dependent on the H3K4me2 mark. We show that Set1, and particularly the H3K4me2 mark, are implicated in repression of a subset of middle sporulation genes during vegetative growth. In the absence of Set1, there is loss of the DNA-binding transcriptional regulator Sum1 and the associated histone deacetylase Hst1 from chromatin in a locus-specific manner. This is linked to increased H4K5ac at these loci and aberrant middle gene expression. These data indicate that, in addition to DNA sequence, histone modification status also contributes to proper localization of Sum1 Our results also show that the role for Set1 in middle gene expression control diverges as cells receive signals to undergo meiosis. Overall, this work dissects an unexplored role for Set1 in gene-specific repression, and provides important insights into a new mechanism associated with the control of gene expression linked to meiotic differentiation. Copyright © 2017 Jaiswal et al.

  5. Decision Taking versus Promise Issuing

    NARCIS (Netherlands)

    Bergstra, J.A.

    2013-01-01

    An alignment is developed between the terminology of outcome oriented decision taking and a terminology for promise issuing. Differences and correspondences are investigated between the concepts of decision and promise. For decision taking, two forms are distinguished: the external outcome

  6. Taking Care of Pressure Sores

    Science.gov (United States)

    ... Tract Infections: Indwelling (Foley) Catheter Taking Care of Pressure Sores [Download this pamphlet: "Taking Care of Pressure ... may not show up right away. Stages of pressure sores and how to care for them: STAGE ...

  7. Census 2000 Places (cen00pl02)

    Data.gov (United States)

    California Department of Resources — Census 2000 Place Names provides a seamless statewide GIS layer of places, including census designated places (CDP), consolidated cities, and incorporated places,...

  8. Adjusting to New Places: International Student Adjustment and Place Attachment

    Science.gov (United States)

    Terrazas-Carrillo, Elizabeth C.; Hong, Ji Y.; Pace, Terry M.

    2014-01-01

    Using data obtained from in-depth semistructured interviews, we examined international students' attachments to place in the local American Midwestern community where they have attended college for at least 2 years. The results of this study suggest that participants engage in a process of renegotiation of meanings attached to new places in ways…

  9. Attachments to the common-place

    DEFF Research Database (Denmark)

    Blok, Anders

    2015-01-01

    on Thévenot's notion of ‘commonality in the plural’, the article shows how a range of personal affinities to the architectural form of the kyō-machiya, positioned as an urban ‘common-place’, serves to coordinate across otherwise divergent interests. By taking seriously the role of attachments to common-places...... change. To understand the dynamic interplay of architecture and community-building in this case, the article stages a theoretical debate on the politics of shared attachments between three proponents of French pragmatic sociology: Bruno Latour, Antoine Hennion, and Laurent Thévenot. Drawing in particular...

  10. Estudos sobre a esporulação de uma amostra de bacillus: IV - Outras evidências sobre a atividade do íon Mn[2+] na esporulação endotrófica Studies on the sporulation of a Bacillus strain: IV - Further evidences of the Mn[2+] ion activity in the endotrophic sporulation

    Directory of Open Access Journals (Sweden)

    Leon Rabinovitch

    1973-01-01

    Full Text Available Foram feitas experimentações om o intuito de se buscar mais evidências sobre a participação do íon Mn[2+] no mecanismo esporogenético de uma amostra de Bacillus licheniformis. Quando as formas vegetativas desta bactéria eram depositadas em um meio mineral, carente de fonte de carbono utilizável, em conjunto com um agente seqüestrante de metais como EDTA, a esporulação endotrófica deixava de ocorrer. Entretanto, a esporulação pôde ser protegida quando as células eram previamente saturadas com um excesso de Mn[2+] exógeno. As formas esporuladas obtidas nas condições estudadas mostraram termorresistência a 85ºC durante 20 minutos.In the present paper the authors bring out more evidences concerning the influence of Mn[2+] ion on the endotrophic sporulation of Bacillus licheniformis-2390. Vegetative cells of this bacteria could not sporulate if they were submited to a sufficient concentration of EDTA. Otherwise, this sporulatio ocurred when the vegetative forms were first protected by an excess of exogenous Mn[2+] of Zn[2+], Fe[2+] and Mg[2+]. Those spores obtained shown thermoresistence to 85ºC during 20 minutes.

  11. Mapping and measuring place attachment

    DEFF Research Database (Denmark)

    Brown, Greg; Raymond, Christopher Mark; Corcoran, Jonathan

    2015-01-01

    The concept of place attachment has been studied extensively across multiple disciplines but only recently with empirical measurement using public participation GIS (PPGIS) and related crowd-sourcing mapping methods. This research trialed a spatially explicit method for identifying place attachment...

  12. Place prioritization for biodiversity content

    Indian Academy of Sciences (India)

    The prioritization of places on the basis of biodiversity content is part of any systematic biodiversity conservation planning process. The place prioritization procedure implemented in the ResNet software package is described. This procedure is primarily based on the principles of rarity and complementarity. Application of the ...

  13. What Place Are You In?

    Science.gov (United States)

    Leary, Barbara Buckett

    1984-01-01

    The interaction place map, a mnemonic device which depicts the five most common places in which humans interact, can be used to teach social skills to elementary learning disabled students. Students can plot movement on the map as they move within an interaction. (CL)

  14. Characterization and performances of DOSION, a dosimetry equipment dedicated to radiobiology experiments taking place at GANIL

    Energy Technology Data Exchange (ETDEWEB)

    Boissonnat, Guillaume, E-mail: boissonnat@lpccaen.in2p3.fr [LPC (Normandie Univ-ENSICAEN-UNICAEN-CNRS/IN2P3), 6 Bd Maréchal Juin, 14050 Caen (France); Fontbonne, Jean-Marc [LPC (Normandie Univ-ENSICAEN-UNICAEN-CNRS/IN2P3), 6 Bd Maréchal Juin, 14050 Caen (France); Balanzat, Emmanuel [CIMAP (CEA/DSM-CNRS/INP-ENSICAEN-UNICAEN), Bd Henri Becquerel, 14076 Caen (France); Boumard, Frederic; Carniol, Benjamin [LPC (Normandie Univ-ENSICAEN-UNICAEN-CNRS/IN2P3), 6 Bd Maréchal Juin, 14050 Caen (France); Cassimi, Amine [CIMAP (CEA/DSM-CNRS/INP-ENSICAEN-UNICAEN), Bd Henri Becquerel, 14076 Caen (France); Colin, Jean; Cussol, Daniel; Etasse, David; Fontbonne, Cathy [LPC (Normandie Univ-ENSICAEN-UNICAEN-CNRS/IN2P3), 6 Bd Maréchal Juin, 14050 Caen (France); Frelin, Anne-Marie [GANIL (CEA/DSM-CNRS/IN2P3), Bd Henri Becquerel, 14076 Caen (France); Hommet, Jean; Salvador, Samuel [LPC (Normandie Univ-ENSICAEN-UNICAEN-CNRS/IN2P3), 6 Bd Maréchal Juin, 14050 Caen (France)

    2017-06-01

    Currently, radiobiology experiments using heavy ions at GANIL (Grand Accélérateur National d′Ions Lourds) are conducted under the supervision of the CIMAP (Center for research on Ions, MAterials and Photonics). In this context, a new beam monitoring equipment named DOSION has been developed. It allows to perform measurements of accurate fluence and dose maps in near real time for each biological sample irradiated. In this paper, we present the detection system, its design, performances, calibration protocol and measurements performed during radiobiology experiments. This setup is currently available for any radiobiology experiments if one wishes to correlate one's own sample analysis to state-of-the-art dosimetric references.

  15. Ac hopping conduction at extreme disorder takes place on the percolating cluster

    DEFF Research Database (Denmark)

    Schrøder, Thomas; Dyre, J. C.

    2008-01-01

    Simulations of the random barrier model show that ac currents at extreme disorder are carried almost entirely by the percolating cluster slightly above threshold; thus contributions from isolated low activation-energy clusters are negligible. The effective medium approximation in conjunction...... with the Alexander-Orbach conjecture lead to an excellent analytical fit to the universal ac conductivity with no nontrivial fitting parameters....

  16. Necroptosis takes place in human immunodeficiency virus type-1 (HIV-1-infected CD4+ T lymphocytes.

    Directory of Open Access Journals (Sweden)

    Ting Pan

    Full Text Available Human immunodeficiency virus type 1 (HIV-1 infection is characterized by progressive depletion of CD4+ T lymphocytes and dysfunction of the immune system. The numbers of CD4+ T lymphocytes in the human body are maintained constantly by homeostatic mechanisms that failed during HIV-1 infection, resulting in progressive loss of CD4+ T cells mainly via apoptosis. Recently, a non-apoptotic form of necrotic programmed cell death, named necroptosis, has been investigated in many biological and pathological processes. We then determine whether HIV-1-infected cells also undergo necroptosis. In this report, we demonstrate that HIV-1 not only induces apoptosis, but also mediates necroptosis in the infected primary CD4+ T lymphocytes and CD4+ T-cell lines. Necroptosis-dependent cytopathic effects are significantly increased in HIV-1-infected Jurkat cells that is lack of Fas-associated protein-containing death domain (FADD, indicating that necroptosis occurs as an alternative cell death mechanism in the absence of apoptosis. Unlike apoptosis, necroptosis mainly occurs in HIV-infected cells and spares bystander damage. Treatment with necrostatin-1(Nec-1, a RIP1 inhibitor that specifically blocks the necroptosis pathway, potently restrains HIV-1-induced cytopathic effect and interestingly, inhibits the formation of HIV-induced syncytia in CD4+ T-cell lines. This suggests that syncytia formation is mediated, at least partially, by necroptosis-related processes. Furthermore, we also found that the HIV-1 infection-augmented tumor necrosis factor-alpha (TNF-α plays a key role in inducing necroptosis and HIV-1 Envelope and Tat proteins function as its co-factors. Taken together,necroptosis can function as an alternative cell death pathway in lieu of apoptosis during HIV-1 infection, thereby also contributing to HIV-1-induced cytopathic effects. Our results reveal that in addition to apoptosis, necroptosis also plays an important role in HIV-1-induced pathogenesis.

  17. Mismatch negativity to acoustical illusion of beat: how and where the change detection takes place?

    Science.gov (United States)

    Chakalov, Ivan; Paraskevopoulos, Evangelos; Wollbrink, Andreas; Pantev, Christo

    2014-10-15

    In case of binaural presentation of two tones with slightly different frequencies the structures of brainstem can no longer follow the interaural time differences (ITD) resulting in an illusionary perception of beat corresponding to frequency difference between the two prime tones. Hence, the beat-frequency does not exist in the prime tones presented to either ear. This study used binaural beats to explore the nature of acoustic deviance detection in humans by means of magnetoencephalography (MEG). Recent research suggests that the auditory change detection is a multistage process. To test this, we employed 26 Hz-binaural beats in a classical oddball paradigm. However, the prime tones (250 Hz and 276 Hz) were switched between the ears in the case of the deviant-beat. Consequently, when the deviant is presented, the cochleae and auditory nerves receive a "new afferent", although the standards and the deviants are heard identical (26 Hz-beats). This allowed us to explore the contribution of auditory periphery to change detection process, and furthermore, to evaluate its influence on beats-related auditory steady-state responses (ASSRs). LORETA-source current density estimates of the evoked fields in a typical mismatch negativity time-window (MMN) and the subsequent difference-ASSRs were determined and compared. The results revealed an MMN generated by a complex neural network including the right parietal lobe and the left middle frontal gyrus. Furthermore, difference-ASSR was generated in the paracentral gyrus. Additionally, psychophysical measures showed no perceptual difference between the standard- and deviant-beats when isolated by noise. These results suggest that the auditory periphery has an important contribution to novelty detection already at sub-cortical level. Overall, the present findings support the notion of hierarchically organized acoustic novelty detection system. Copyright © 2014 Elsevier Inc. All rights reserved.

  18. Using a Very Big Rocket to take Very Small Satellites to Very Far Places

    Science.gov (United States)

    Cohen, Barbara

    2017-01-01

    Planetary science cubesats are being built. Insight (2018) will carry 2 cubesats to provide communication links to Mars. EM-1 (2019) will carry 13 cubesat-class missions to further smallsat science and exploration capabilities. Planetary science cubesats have more in common with large planetary science missions than LEO cubesats- need to work closely with people who have deep-space mission experience

  19. Concholepas hemocyanin biosynthesis takes place in the hepatopancreas, with hemocytes being involved in its metabolism.

    Science.gov (United States)

    Manubens, Augusto; Salazar, Fabián; Haussmann, Denise; Figueroa, Jaime; Del Campo, Miguel; Pinto, Jonathan Martínez; Huaquín, Laura; Venegas, Alejandro; Becker, María Inés

    2010-12-01

    Hemocyanins are copper-containing glycoproteins in some molluscs and arthropods, and their best-known function is O(2) transport. We studied the site of their biosynthesis in the gastropod Concholepas concholepas by using immunological and molecular genetic approaches. We performed immunohistochemical staining of various organs, including the mantle, branchia, and hepatopancreas, and detected C. concholepas hemocyanin (CCH) molecules in circulating and tissue-associated hemocytes by electron microscopy. To characterize the hemocytes, we purified them from hemolymph. We identified three types of granular cells. The most abundant type was a phagocyte-like cell with small cytoplasmic granules. The second type contained large electron-dense granules. The third type had vacuoles containing hemocyanin molecules suggesting that synthesis or catabolism occurred inside these cells. Our failure to detect cch-mRNA in hemocytes by reverse transcription with the polymerase chain reaction (RT-PCR) led us to propose that hemocytes instead played a role in CCH metabolism. This hypothesis was supported by colloidal gold staining showing hemocyanin molecules in electron-dense granules inside hemocytes. RT-PCR analysis, complemented by in situ hybridization analyses with single-stranded antisense RNAs as specific probes, demonstrated the presence of cch-mRNA in the hepatopancreas; this was consistent with the specific hybridization signal and confirmed the hepatopancreas as the site of CCH synthesis. Finally, we investigated the possibility that CCH catabolism in hemocytes was involved in the host immune response and in the generation of secondary metabolites such as antimicrobial peptides and phenoloxidase.

  20. The Eclipsing Binary Di Herculis: One Mystery Solved, But Another Takes Its Place

    Science.gov (United States)

    Zimmerman, Nicole; Guinan, E.; Maloney, F.

    2010-01-01

    The 8th-mag eclipsing binary DI Herculis has perplexed scientists for the past few decades due to its anomalously slow apsidal motion rate. DI Her consists of two main-sequence stars (B5V, B6V), with P(orb) = 10.55 days, and eccentricity(e= 0.489). Since the apsidal motion is dominated by General Relativity, the system is one of the few tests available for verifying the theory. Combining the expected classical (1.93°/100 yr) and relativistic (2.34°/100 yr) effects, the predicted apsidal motion rate is 4.27°/100 yr. Our recent determination of the apsidal motion yields 1.33°+/-0.25 /100 yr, based on eclipse timings from 1936-2008. Recently, Albrecht et al (2009, Nature 461) have apparently solved the apsidal motion anomaly of DI Her, finding that the axes of both stars are significantly inclined from the normal to the orbital plane. This was determined from the radial velocity curves and observing the Rossiter-McLaughlin effect during primary and secondary eclipses. Having significantly misaligned axes of rotation produces a perturbation that greatly reduces the classical apsidal motion effect, thus explaining the observed small apsidal motion rate. Even though this discovery apparently solves the problem, it raises new questions as to how the axes are so tilted. Additionally, tilted axes are expected to contribute to other orbital effects, such as changes in orbital inclination, which have not yet observed from the apparent constancy in eclipse depths over time. We have also searched for evidence of small periodic oscillations in the eclipse timings and found no evidence of a light travel time effect arising from a possible tertiary component. Further, we find evidence that the projected rotation axes of the stars may be precessing, since it appears that the value of V(rot)sini has increased over the past 30 years. This research was supported by NSF/RUI Grants AST05-07536/42.

  1. Simulations: A Safe Place to Take Risks in Discussing Cultural Differences.

    Science.gov (United States)

    Pedersen, Paul

    1995-01-01

    Describes examples of simulations and strategies for teaching about psychology that were developed in Indonesia and Malaysia to incorporate and address controversies related to cultural differences. A model that simulates the influence of a client's cultural context in counseling is explained, and developing stereotyped synthetic cultures is…

  2. Subsurface nitrate reduction under wetlands takes place in narrow superficial zones

    DEFF Research Database (Denmark)

    dos Santos Ribas, Osmar; Calderer, M.; Marti, Vicens

    2017-01-01

    and the performance of a re-established wetland. Intact soil cores were collected and divided in slices for the determination of Organic Matter (OM) through Loss of Ignition (LOI) as well as Dissolved Organic Carbon (DOC) and NRP spiking nitrate in batch tests. The Nitrate Reduction (NR) was fitted as a pseudo......This study aims to investigate the depth distribution of the Nitrate Reduction Potential (NRP) on a natural and a re-established wetland. The obtained NRP provides a valuable data of the driving factors affecting denitrification, the Dissimilatory Nitrate Reduction to Ammonium (DNRA) process...... with NRP, high amounts of DOC ensured enough quantity and quality of labile OM for NR. Besides, high concentration of LOI but a scarce abundance of DOC failed to drive NR. DNRA was only important in superficial samples with high contents of OM. Lastly, the high NRP of the re-established wetland confirms...

  3. Taking Stock, Placing Orders: A Historiographic Essay on the Business History of the Newspaper.

    Science.gov (United States)

    Smith, Carol; Dyer, Carolyn Stewart

    This historiographic essay discusses and seeks to begin to address the need for a business history of the newspaper. The paper's first section clarifies the terms "business history,""economic history,""political history," and "history of economics," (which the paper suggests have been used interchangeably in…

  4. AN EVALUATION OF WATER QALITY OF AKPINAR STREAM (DENİZLİ WHERE TROUT PRODUCTION TAKES PLACE

    Directory of Open Access Journals (Sweden)

    Esengül KÖSE

    2012-08-01

    Full Text Available In this study, 20 physicochemical parameters were measured mountly in 2007 and seasonaly in 2008 and 2009 on Akpınar Stream, which is located within the borders of Denizli and has 13 trout production facility. The data obtained were evaluated according to the fresh water fish directive which was agreed by the Commision of European Union (EC Directives and Water Pollution Control Regulation Criteria. We determined that, the measured parameters on the first station do not constitute any risk for Salmonids and Cyprinids according to EC Directives and in the second station, the valuesof biological oxygen demand and nitrite were high enough to adversely affect the health of fish. There is not a significant difference between two stations for the parameters of oxygen, nitrate, totalnitrogen, pH, chemical oxygen demand, acid binding capacity, total hardness and chlorine. The parametes of total suspended solids, ammonium, nitrite, secondary phosphate, total phosphorus, organicmatter, ammonia, biological oxygen demand, turbidity, temperature and flow rate were significantly high in second station (p<0.05. The reason of high levels of the parameters especially origin of organic pollution in the secod station is fish feces and feed wastes which directly given into the water. Settling ponds should be used properly and controls should be provided to increase for more healtyproduction.

  5. Last piece of ATLAS takes the plunge

    CERN Document Server

    2008-01-01

    On Friday 29 February the second small wheel was lowered 100 metres underground into the ATLAS cavern in front of a captivated audience. Although called the "small wheels" they are small in name only - each wheel is 9.3 metres in diameter and weighs 100 tonnes including the massive shielding elements. This piece completes ATLAS’ muon spectrometer, which has the ability to accurately track particles to the width of a human hair. The first piece of ATLAS was installed in 2003 and, five years later, this small wheel is the final large piece of the detector to take the subterranean voyage to its final resting place.

  6. Place recognition using batlike sonar.

    Science.gov (United States)

    Vanderelst, Dieter; Steckel, Jan; Boen, Andre; Peremans, Herbert; Holderied, Marc W

    2016-08-02

    Echolocating bats have excellent spatial memory and are able to navigate to salient locations using bio-sonar. Navigating and route-following require animals to recognize places. Currently, it is mostly unknown how bats recognize places using echolocation. In this paper, we propose template based place recognition might underlie sonar-based navigation in bats. Under this hypothesis, bats recognize places by remembering their echo signature - rather than their 3D layout. Using a large body of ensonification data collected in three different habitats, we test the viability of this hypothesis assessing two critical properties of the proposed echo signatures: (1) they can be uniquely classified and (2) they vary continuously across space. Based on the results presented, we conclude that the proposed echo signatures satisfy both criteria. We discuss how these two properties of the echo signatures can support navigation and building a cognitive map.

  7. Strategies for Aging in Place

    Directory of Open Access Journals (Sweden)

    Suzanne Dupuis-Blanchard

    2015-01-01

    Full Text Available For healthy and independent older adults, aging in place can be seen as identical to any other adult living at home. Little is known about how frail seniors, particularly those who speak a minority language, manage the challenges of aging in place. The present qualitative descriptive study explores the strategies that Canadian French-speaking seniors have put in place to counter their loss of independence and promote their ability to stay in their home. Semistructured individual interviews were conducted with 39 older adults and transcribed, followed by content analysis to identify common themes related to study objectives. Six themes emerged in response to strategies described for aging in place. Findings reveal the limited extent to which language issues were perceived as a barrier by participants. In conclusion, the results of this study provide us with fruitful insights to guide community nursing practice, future research, and public policy.

  8. An Austere Place of Refuge

    Czech Academy of Sciences Publication Activity Database

    Feinberg, Joseph Grim

    -, February 15 (2016), s. 1-8 Institutional support: RVO:67985955 Keywords : refugee crisis * neoliberalism * extreme right Subject RIV: AA - Philosophy ; Religion http://www.criticatac.ro/lefteast/an-austere-place-of-refuge/

  9. Upcoming training sessions (up to end October) - Places available

    CERN Multimedia

    2016-01-01

    Please find below a list of training sessions scheduled to take place up to the end of October with places available.   Safety and Language courses are not included here, you will find an up-to-date list in the Training Catalogue. If you need a course which is not featured  in the catalogue, please contact one of the following: your supervisor, your Departmental Training Officer or the relevant learning specialist.  

  10. The Place and Importance of Playgrounds in Child Development

    OpenAIRE

    Merve ÜNAL

    2009-01-01

    Education is an activity that is not restricted to the school areas, sometimes goes beyond the school walls. Similar to schools, various institutions or establishments like playgrounds, libraries, and museums also take place in educational and/or instructional process. Among these places, playgrounds can be regarded as critically important in terms of discharging children’s high energy, positive self (ego) development, exploring skills, self esteem and success of children, and also cognitive...

  11. HOMING PLACE: TOWARDS A PARTICIPATORY, AMBULANT AND CONVERSIVE METHODOLOGY

    OpenAIRE

    Myers, Misha

    2009-01-01

    The practice-as-research project Homing Place proposes a transferable percipient-led methodology of performance and research activated by ambulant and conversive mechanisms as the culmination of this research. The thesis is comprised of a range of activity that represents a moment and way of writing practice. Three artworks that comprise part of the practical component of this thesis--- way from home, Take me to a place and Yodel Rodeo-- each involved participation and contribu...

  12. Communication course – places available

    CERN Multimedia

    2012-01-01

    Please note that there are some places available in the following communication course starting in November. For more information on the course, click on the course title, this will bring you to the training catalogue. You can then sign-up on line. For advice, you can contact Kerstin Fuhrmeister (70896) or kerstin.fuhrmeister@cern.ch Course Next session Duration Language Availability Gestion de temps 22 November 3 days French 8 places  

  13. Marketing research and place image

    Directory of Open Access Journals (Sweden)

    Miroslav Foret

    2006-01-01

    Full Text Available The paper deals with some problems of the image and identity of two towns (Brno and Zlín as well as the whole country (Czech Republic. It starts with theoretical explanation of terms place image, identity and culture and its interrelated influences. Than own empirical marketing research of Brno image is presented and compared with theory of marketing communication, especially with the problems of place identity, unified visual style and logo.

  14. Naturalness and Place in River Rehabilitation

    Directory of Open Access Journals (Sweden)

    Kirstie Fryirs

    2009-06-01

    Full Text Available An authentic approach to river rehabilitation emphasizes concerns for the natural values of a given place. As landscape considerations fashion the physical template upon which biotic associations take place, various geomorphic issues must be addressed in framing rehabilitation activities that strive to improve river health. An open-ended approach to river classification promotes applications that appreciate the values of a given river, rather than pigeonholing reality. As the geomorphic structure of some rivers is naturally simple, promoting heterogeneity as a basis for management may not always be appropriate. Efforts to protect unique attributes of river systems must be balanced with procedures that look after common features. Concerns for ecosystem functionality must relate to the behavioral regime of a given river, remembering that some rivers are inherently sensitive to disturbance. Responses to human disturbance must be viewed in relation to natural variability, recognizing how spatial relationships in a catchment, and responses to past disturbances, fashion the operation of contemporary fluxes. These fluxes, in turn, influence what is achievable in the rehabilitation of a given reach. Given the inherently adjusting and evolutionary nature of river systems, notional endpoints do not provide an appropriate basis upon which to promote concepts of naturalness and place in the rehabilitation process. These themes are drawn together to promote rehabilitation practices that relate to the natural values of each river system, in preference to applications of "cookbook" measures that build upon textbook geomorphology.

  15. Note Taking for Geography Students.

    Science.gov (United States)

    Kneale, Pauline E.

    1998-01-01

    Addresses geography students' questions about why, when, and how to take notes. Outlines a step-by-step process for taking notes from written sources and from class lectures. Discusses what types of notes are appropriate for various types of sources. Suggests some ideas for making notes useful for individual learning styles. (DSK)

  16. Informatics perspectives on decision taking

    NARCIS (Netherlands)

    Bergstra, J.A.

    2011-01-01

    A decision is an act or event of decision taking. Decision making always includes decision taking, the latter not involving significant exchanges with non-deciding agents. A decision outcome is a piece of storable information constituting the result of a decision. Decision outcomes are typed, for

  17. Taking Chances in Romantic Relationships

    Science.gov (United States)

    Elliott, Lindsey; Knox, David

    2016-01-01

    A 64 item Internet questionnaire was completed by 381 undergraduates at a large southeastern university to assess taking chances in romantic relationships. Almost three fourths (72%) self-identified as being a "person willing to take chances in my love relationship." Engaging in unprotected sex, involvement in a "friends with…

  18. Take Charge. Take the Test. "You Know" PSA (:60)

    Centers for Disease Control (CDC) Podcasts

    2012-03-07

    As part of the Take Charge. Take the Test. campaign, this 60 second PSA encourages African American women to get tested for HIV. Locations for a free HIV test can be found by visiting hivtest.org/takecharge or calling 1-800-CDC-INFO (1-800-232-4636).  Created: 3/7/2012 by National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention (NCHHSTP).   Date Released: 3/7/2012.

  19. Patterns of place promotion, place marketing and/or place branding in Dutch municipalities

    NARCIS (Netherlands)

    Boisen, Martin; Groote, Peter; Terlouw, Kees; Couwenberg, Oscar

    This paper analyses the different degrees to which place promotion, marketing and branding policies are institutionalised, based on a relatively straightforward and generally applicable methodology in order to stimulate international comparative research in the field. A consensus has emerged over

  20. Aag Hypoxanthine-DNA Glycosylase Is Synthesized in the Forespore Compartment and Involved in Counteracting the Genotoxic and Mutagenic Effects of Hypoxanthine and Alkylated Bases in DNA during Bacillus subtilis Sporulation.

    Science.gov (United States)

    Ayala-García, Víctor M; Valenzuela-García, Luz I; Setlow, Peter; Pedraza-Reyes, Mario

    2016-12-15

    Aag from Bacillus subtilis has been implicated in in vitro removal of hypoxanthine and alkylated bases from DNA. The regulation of expression of aag in B. subtilis and the resistance to genotoxic agents and mutagenic properties of an Aag-deficient strain were studied here. A strain with a transcriptional aag-lacZ fusion expressed low levels of β-galactosidase during growth and early sporulation but exhibited increased transcription during late stages of this developmental process. Notably, aag-lacZ expression was higher inside the forespore than in the mother cell compartment, and this expression was abolished in a sigG-deficient background, suggesting a forespore-specific mechanism of aag transcription. Two additional findings supported this suggestion: (i) expression of an aag-yfp fusion was observed in the forespore, and (ii) in vivo mapping of the aag transcription start site revealed the existence of upstream regulatory sequences possessing homology to σ G -dependent promoters. In comparison with the wild-type strain, disruption of aag significantly reduced survival of sporulating B. subtilis cells following nitrous acid or methyl methanesulfonate treatments, and the Rif r mutation frequency was significantly increased in an aag strain. These results suggest that Aag protects the genome of developing B. subtilis sporangia from the cytotoxic and genotoxic effects of base deamination and alkylation. In this study, evidence is presented revealing that aag, encoding a DNA glycosylase implicated in processing of hypoxanthine and alkylated DNA bases, exhibits a forespore-specific pattern of gene expression during B. subtilis sporulation. Consistent with this spatiotemporal mode of expression, Aag was found to protect the sporulating cells of this microorganism from the noxious and mutagenic effects of base deamination and alkylation. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  1. Worst places to practice medicine

    Directory of Open Access Journals (Sweden)

    Robbins RA

    2017-05-01

    Full Text Available No abstract available. Article truncated after 150 words. Medscape periodically publishes a “Best” and “Worst” places to practice medicine (1. We were struck by this year’s list because three of the five worst places to practice medicine are in the Southwest (Table 1. Table 1. Medscape’s “worst” places to practice medicine. 1. New Orleans, Louisiana; 2.\tPhoenix, Arizona; 3. Las Vegas, Nevada; 4. Albuquerque, New Mexico; 5. Tulsa, Oklahoma. While Minneapolis rated the best place to practice, only 2 cities from the Southwest made the top 25 “Best” list-Salt Lake City at 13th and Colorado Springs at 24th. Most of the top 25 are from the Midwest or Northeast. None from California made the best places list and only the only Southern location was Virginia Beach, Virginia. Rankings resulted from the combination of twelve 50-state rankings: medical board actions per doctor; malpractice lawsuits per doctor; office-based primary care physicians per population; physician income; employer-based insurance rate per population …

  2. ENSEIGNEMENT TECHNIQUE TECHNICAL TRAINING Places disponibles*/Places available **

    CERN Document Server

    2003-01-01

    * Etant donné le délai d'impression du Bulletin, ces places peuvent ne plus être disponibles au moment de sa parution. Veuillez consulter notre site Web pour avoir la dernière mise à jour. ** The number of places available may vary. Please check our Web site to find out the current availability. Des places sont disponibles dans les cours suivants : Places are available in the following courses : Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) CLEAN-2002 : Working in a Cleanroom : 23.10.03 (half day, free of charge) Plannification de projet avec MS-Project/Project Scheduling with MS-Project : 2 sessions : 23.10 & 4.11.03 and 18 &25.11.03 (2 jours/2 days - langue à définir/language to be defined) AutoCAD 2002 – Level 1 : 3, 4, 12, 13.11.03 (4 days) Introduction to Pspice : 4.11...

  3. The Path Is Place: Skateboarding, Graffiti and Performances of Place

    Science.gov (United States)

    Ong, Adelina

    2016-01-01

    This article reflects on two performances of place involving graffiti and skateboarding: the first looks at a graffiti intervention by SKL0, an urban artist in Singapore, and the second examines the "Long Live Southbank" ("LLSB") campaign to resist the relocation of Southbank's Undercroft, an appropriated skate space in London.…

  4. Without Place, Is It Real?

    Directory of Open Access Journals (Sweden)

    Joseph F Donnermeyer

    2016-09-01

    Full Text Available This article reviews Matthews’ (2014 Realist Criminology as an opportunity to address larger shortcomings within critical criminology, which is the failure to develop an alternative theory of crime and place to the mainstream theories of social disorganisation and collective efficacy. It uses rural criminological work related to violence against women and substance use, production and trafficking to illustrate the importance of place for development of a realist criminology that can consider localised expressions of power and inequality, and the multiplicity of networks and roles by which people can simultaneously be involved in both conforming and deviant/criminal behaviours. The article also suggests that a critical theory of crime and place would be useful to the synthesis and re-interpretation of criminological literature that is either theory-less or lacks a critical perspective.

  5. Effects of Two Salts Compounds on Mycelial Growth, Sporulation, and Spore Germination of Six Isolates of Botrytis cinerea in the Western North of Algeria

    Directory of Open Access Journals (Sweden)

    Boualem Boumaaza

    2015-01-01

    Full Text Available Six isolates of Botrytis cinerea were isolated from leaves and stems of different tomato varieties taken from four areas in the northwest of Algeria where tomato is mostly grown in greenhouses and high tunnels. The purpose of this research was to determine the effect of two salts, NaCl and CaCl2, on three stages of Botrytis cinerea’s life cycle. All isolates tested were stimulated in 50 to 150 ppm; NaCl was the most effective treatment to increase mycelial growth at two tested concentrations. However, at 300 ppm concentration, CaCl2 completely inhibited the growth of mycelium; they reach 34.78% for the isolate TR46 and 26.72% for isolate F27. The sodium and calcium salts stimulated conidia production in liquid culture. We noticed that the effect of calcium chloride on sporulation was average while sodium chloride. In the medium containing 50 ppm, calcium chloride and sodium chloride increased the germination capacity of most isolates compared with the control. Other calcium salts, at 100 or 300 ppm, decreased the germination percentage of the conidia. With the exception of sodium salts, the inhibitions of germination reduce at 150 or 300 compared with the control. Conidial germination was slightly inhibited by sodium chloride only when the concentration was over 300 ppm.

  6. Effects of Two Salts Compounds on Mycelial Growth, Sporulation, and Spore Germination of Six Isolates of Botrytis cinerea in the Western North of Algeria

    Science.gov (United States)

    Boumaaza, Boualem; Benkhelifa, Mohamed; Belkhoudja, Moulay

    2015-01-01

    Six isolates of Botrytis cinerea were isolated from leaves and stems of different tomato varieties taken from four areas in the northwest of Algeria where tomato is mostly grown in greenhouses and high tunnels. The purpose of this research was to determine the effect of two salts, NaCl and CaCl2, on three stages of Botrytis cinerea's life cycle. All isolates tested were stimulated in 50 to 150 ppm; NaCl was the most effective treatment to increase mycelial growth at two tested concentrations. However, at 300 ppm concentration, CaCl2 completely inhibited the growth of mycelium; they reach 34.78% for the isolate TR46 and 26.72% for isolate F27. The sodium and calcium salts stimulated conidia production in liquid culture. We noticed that the effect of calcium chloride on sporulation was average while sodium chloride. In the medium containing 50 ppm, calcium chloride and sodium chloride increased the germination capacity of most isolates compared with the control. Other calcium salts, at 100 or 300 ppm, decreased the germination percentage of the conidia. With the exception of sodium salts, the inhibitions of germination reduce at 150 or 300 compared with the control. Conidial germination was slightly inhibited by sodium chloride only when the concentration was over 300 ppm. PMID:25883657

  7. Inferring Biological Mechanisms by Data-Based Mathematical Modelling: Compartment-Specific Gene Activation during Sporulation in Bacillus subtilis as a Test Case.

    Science.gov (United States)

    Iber, Dagmar

    2011-01-01

    Biological functionality arises from the complex interactions of simple components. Emerging behaviour is difficult to recognize with verbal models alone, and mathematical approaches are important. Even few interacting components can give rise to a wide range of different responses, that is, sustained, transient, oscillatory, switch-like responses, depending on the values of the model parameters. A quantitative comparison of model predictions and experiments is therefore important to distinguish between competing hypotheses and to judge whether a certain regulatory behaviour is at all possible and plausible given the observed type and strengths of interactions and the speed of reactions. Here I will review a detailed model for the transcription factor σ(F), a regulator of cell differentiation during sporulation in Bacillus subtilis. I will focus in particular on the type of conclusions that can be drawn from detailed, carefully validated models of biological signaling networks. For most systems, such detailed experimental information is currently not available, but accumulating biochemical data through technical advances are likely to enable the detailed modelling of an increasing number of pathways. A major challenge will be the linking of such detailed models and their integration into a multiscale framework to enable their analysis in a larger biological context.

  8. Inferring Biological Mechanisms by Data-Based Mathematical Modelling: Compartment-Specific Gene Activation during Sporulation in Bacillus subtilis as a Test Case

    Directory of Open Access Journals (Sweden)

    Dagmar Iber

    2011-01-01

    Full Text Available Biological functionality arises from the complex interactions of simple components. Emerging behaviour is difficult to recognize with verbal models alone, and mathematical approaches are important. Even few interacting components can give rise to a wide range of different responses, that is, sustained, transient, oscillatory, switch-like responses, depending on the values of the model parameters. A quantitative comparison of model predictions and experiments is therefore important to distinguish between competing hypotheses and to judge whether a certain regulatory behaviour is at all possible and plausible given the observed type and strengths of interactions and the speed of reactions. Here I will review a detailed model for the transcription factor , a regulator of cell differentiation during sporulation in Bacillus subtilis. I will focus in particular on the type of conclusions that can be drawn from detailed, carefully validated models of biological signaling networks. For most systems, such detailed experimental information is currently not available, but accumulating biochemical data through technical advances are likely to enable the detailed modelling of an increasing number of pathways. A major challenge will be the linking of such detailed models and their integration into a multiscale framework to enable their analysis in a larger biological context.

  9. Should You Take Dietary Supplements?

    Science.gov (United States)

    ... 2013 Print this issue Should You Take Dietary Supplements? A Look at Vitamins, Minerals, Botanicals and More ... Gut in Check Wise Choices Safe Use of Supplements Tell all of your health care providers about ...

  10. Take Care with Pet Reptiles

    Science.gov (United States)

    ... Submit Button Past Emails Take Care with Pet Reptiles and Amphibians Language: English (US) Español (Spanish) Recommend ... pets and disease risks Safe Handling Tips for Reptiles and Amphibians Always wash your hands thoroughly after ...

  11. Taking Action for Healthy Kids.

    Science.gov (United States)

    Kidd, Jill E.

    2003-01-01

    Summarizes research on relationship between physical activity, good nutrition, and academic performance. Offers several recommendations for how schools can take action to improve the nutrition and fitness of students. (PKP)

  12. Informatics perspectives on decision taking

    OpenAIRE

    Bergstra, J.A.

    2011-01-01

    A decision is an act or event of decision taking. Decision making always includes decision taking, the latter not involving significant exchanges with non-deciding agents. A decision outcome is a piece of storable information constituting the result of a decision. Decision outcomes are typed, for instance: plan, command, assertion, or boolean reply to a question. A decision effect is any consequence of putting a decision outcome into effect. Decision outcomes must be expected by the decider t...

  13. Places available - Technical training (up to the end of 2015)

    CERN Multimedia

    2015-01-01

    Please find below all the upcoming courses (until December) that are currently missing participants, required for the courses to take place.     *Click on the picture*   For more details about a course and to register, please go to the Training Catalogue.

  14. Thrown Together: Incorporating Place and Sustainability into Early Literacy Education

    Science.gov (United States)

    Schmidt, Catarina

    2017-01-01

    The development of language and literacy abilities of young multilingual children is important to their future educational engagement and success in school. In this study, the value of taking account of place and sustainability in early literacy education is considered. This research provides ideas for practice-based research on early literacy in…

  15. After Dark in the Antipodes: Pedagogy, Place and Queer Phenomenology

    Science.gov (United States)

    Crowley, Vicki; Rasmussen, Mary Lou

    2010-01-01

    This paper pursues issues of pedagogy, place and queer phenomenology in the context of what might be meant by the term "after-queer" or "what falls outside queer" as we currently theorise, practice and locate queer. Inspired by Sara Ahmed's account of how bodies become oriented by the ways in which they take up time and space,…

  16. A Place on the Shelf

    Science.gov (United States)

    Thomas, Devon

    2007-01-01

    If we read to discover new worlds, we also read to find ourselves. For gays and lesbians, this act of discovery can be problematic: literature has so often excluded them. In the last decades, as gays and lesbians have grown increasingly vocal in the effort to secure their rightful place in society, a broad range of fiction has emerged that…

  17. No place for their children:

    DEFF Research Database (Denmark)

    Nielsen, Helene Pristed

    2018-01-01

    or circumstance) include flexible hours and high levels of mobility reveal explicit hopes that their children will have more stable working lives – and expectations that this will mean that they shall have to leave the local area. The article documents how the local place is an important component...

  18. Management and communication courses – Places available

    CERN Multimedia

    2014-01-01

    Please note that there are some places available on the following management and communication courses starting in September or October.   For more information on the course, click on the course title, which will take you to the training catalogue. You can then sign up on line.  .tftable {color:#333333;width:100%;border-width: 1px;border-color: #a9a9a9;border-collapse: collapse;} .tftable th {background-color:#cdcdcd;border-width: 1px;padding: 8px;border-style: solid;border-color: #a9a9a9;text-align:left;} .tftable tr {background-color:#ffffff;} .tftable td {border-width: 1px;padding: 8px;border-style: solid;border-color: #a9a9a9;} Course Next session Duration Language Availability Managing Time 15 September 9 October 7 November 20 hours English 2 Managing Stress 6 October 7 October 16 hours English 5  

  19. REMINDER: Alcohol and the work place

    CERN Multimedia

    2004-01-01

    The CERN Medical Service has observed an increase in the number of personnel suffering from alcohol-related problems in recent years, in spite of the implementation of stricter regulations concerning the consumption of alcohol on the site. The causes of alcohol-related problems are often complex and many-faceted. A family history of alcohol abuse can be a cofactor in excessive drinking. The effects on a person's work are not negligible and should not be ignored. "Alcohol and the work place" is the third part of a campaign designed to raise awareness of the risks of alcohol consumption, which has already dealt with "alcohol and health" and "alcohol and road safety". Our campaign this year will be rounded off by three information sessions in which a representative of the ISPA (Swiss Institute for the Prevention of Alcoholism) will participate. The last of these sessions will take place in: the Main Building Auditorium on Wednesday, 8 December 2004 from 14:00 to 15:30. Sessions are open to everyone. For m...

  20. Teaching Kids with Learning Disabilities to Take Public Transit

    Science.gov (United States)

    Schoenfeld, Jane

    2009-01-01

    Taking public transit can make anyone nervous, especially in a large or medium-sized city where there are many different bus lines going many different places. The author's daughter, Anna, has multiple learning disabilities and may never learn to drive, but she wants to be as independent as possible so the author taught her to ride the bus. This…