WorldWideScience

Sample records for sporulation takes place

  1. Taking place, screening place

    DEFF Research Database (Denmark)

    Hansen, Kim Toft; Waade, Anne Marit

    2019-01-01

    We introduce location studies as a new empirical approach to screen studies. Location studies represent an interdisciplinary perspective, including media, aesthetics and geography, and reflect a growing interest in places in a global media and consumption culture. The chapter analyses two recent......) with one being traditional and the other being commercial; both dramas include discussions of localities and social heritage, and both use local sports as a common metaphor for social cohesion; and both series have been partly funded by a local film Danish commissioner. However, The Legacy is shot...... to a large extent in studios, while Norskov is shot entirely on location. The study is based on interviews with producers, broadcasters, location scouts, production designers and writers, as well as quantitative and qualitative textual analyses of television drama series, the geographical places, and related...

  2. Then Daddy Takes His Place in an Australian Landscape

    OpenAIRE

    ERIN GAYLE CROUCH

    2018-01-01

    This writing accompanies a 62 minute, single channel, digital film work also titled Then Daddy Takes His Place in an Australian Landscape. The research considers themes of loss and disorientation in the above film and the film practice of Belgian filmmaker Chantal Akerman. Formally, the following writing interweaves critical engagements with Akerman’s films including No Home Movie (2015), Jeanne Dielman, 23 Commerce Quay, 1080 Brussels(1975), Là Bas (2006), with personal stories and reflecti...

  3. Mathematical modeling of phase interaction taking place in materials processing

    International Nuclear Information System (INIS)

    Zinigrad, M.

    2002-01-01

    The quality of metallic products depends on their composition and structure. The composition and the structure are determined by various physico-chemical and technological factors. One of the most important and complicated problems in the modern industry is to obtain materials with required composition, structure and properties. For example, deep refining is a difficult task by itself, but the problem of obtaining the material with the required specific level of refining is much more complicated. It will take a lot of time and will require a lot of expanses to solve this problem empirically and the result will be far from the optimal solution. The most effective way to solve such problems is to carry out research in two parallel direction. Comprehensive analysis of thermodynamics, kinetics and mechanisms of the processes taking place at solid-liquid-gaseous phase interface and building of the clear well-based physico-chemical model of the above processes taking into account their interaction. Development of mathematical models of the specific technologies which would allow to optimize technological processes and to ensure obtaining of the required properties of the products by choosing the optimal composition of the raw materials. We apply the above unique methods. We developed unique methods of mathematical modeling of phase interaction at high temperatures. These methods allows us to build models taking into account: thermodynamic characteristics of the processes, influence of the initial composition and temperature on the equilibrium state of the reactions, kinetics of homogeneous and heterogeneous processes, influence of the temperature, composition, speed of the gas flows, hydrodynamic and thermal factors on the velocity of the chemical and diffusion processes. The models can be implemented in optimization of various metallurgical processes in manufacturing of steels and non-ferrous alloys as well as in materials refining, alloying with special additives

  4. Does olfactory specific satiety take place in a natural setting?

    Science.gov (United States)

    Fernandez, P; Bensafi, M; Rouby, C; Giboreau, A

    2013-01-01

    Olfactory-specific satiety (OSS) is characterized by a specific decrease in the odor pleasantness of a food eaten to satiety or smelled without ingestion. The usual protocol for studying OSS takes place in laboratory, a setting rather removed from the real world. Here, we set out to examine OSS in a natural setting: during a meal in a restaurant. We hypothesized that an aroma contained in a food that is eaten at the beginning of a meal decreases the pleasantness of the flavor of a food with the same aroma eaten at the end of the meal. In the first experiment (Experiment 1), a test group received an appetizer flavored with a test aroma (anise) at the beginning of the meal. After the main dish, they received a dessert flavored with the same aroma. A control group received the same aromatized dessert, but after a non-aromatized appetizer. This experiment was replicated (Experiment 2) using verbena as the test aroma. For both experiments, results revealed that aroma pleasantness, but not intensity or familiarity, significantly decreased in the test groups vs. the control groups. These findings extend the concept of OSS to a realistic eating context. Copyright © 2012 Elsevier Ltd. All rights reserved.

  5. Key European Grid event to take place in Geneva

    CERN Multimedia

    2006-01-01

    EGEE'06 is the main conference of the EGEE project, which is co-funded by the European Union and hosted by CERN. More than 90 partners all over Europe and beyond are working together in EGEE to provide researchers in both academia and industry with access to major computing resources, independent of their geographic location. The largest user community of the EGEE Grid is the High-Energy Physics community and in particular the LHC experiments, which are already making heavy use of the infrastructure to prepare for data taking. However, with the many new challenges faced by EGEE in its second phase that started in April this year, an even broader audience than at previous EGEE conferences is expected. In particular, a large number of related Grid projects will feature prominently in both plenary and parallel sessions during the 5 days of this event. Industry will also be well represented, highlighting the EGEE project's commitment to technology transfer to industry. CERN is the host of the conference, which i...

  6. Sporulation in Bacteria: Beyond the Standard Model.

    Science.gov (United States)

    Hutchison, Elizabeth A; Miller, David A; Angert, Esther R

    2014-10-01

    Endospore formation follows a complex, highly regulated developmental pathway that occurs in a broad range of Firmicutes. Although Bacillus subtilis has served as a powerful model system to study the morphological, biochemical, and genetic determinants of sporulation, fundamental aspects of the program remain mysterious for other genera. For example, it is entirely unknown how most lineages within the Firmicutes regulate entry into sporulation. Additionally, little is known about how the sporulation pathway has evolved novel spore forms and reproductive schemes. Here, we describe endospore and internal offspring development in diverse Firmicutes and outline progress in characterizing these programs. Moreover, comparative genomics studies are identifying highly conserved sporulation genes, and predictions of sporulation potential in new isolates and uncultured bacteria can be made from these data. One surprising outcome of these comparative studies is that core regulatory and some structural aspects of the program appear to be universally conserved. This suggests that a robust and sophisticated developmental framework was already in place in the last common ancestor of all extant Firmicutes that produce internal offspring or endospores. The study of sporulation in model systems beyond B. subtilis will continue to provide key information on the flexibility of the program and provide insights into how changes in this developmental course may confer advantages to cells in diverse environments.

  7. Optimizing sporulation of Clostridium perfringens

    NARCIS (Netherlands)

    Jong, de A.E.I.; Beumer, R.R.; Rombouts, F.M.

    2002-01-01

    Many sporulation media have been developed for Clostridium perfringens, but none stimulates sporulation for all strains. The aim of our experiments was to develop a sporulation method using Duncan and Strong (DS) medium, which supports sporulation of a wide variety of strains. Different inoculation

  8. Pirated Siderophores Promote Sporulation in Bacillus subtilis.

    Science.gov (United States)

    Grandchamp, Gabrielle M; Caro, Lews; Shank, Elizabeth A

    2017-05-15

    In microbial communities, bacteria chemically and physically interact with one another. Some of these interactions are mediated by secreted specialized metabolites that act as either intraspecies or interspecies signals to alter gene expression and to change cell physiology. Bacillus subtilis is a well-characterized soil microbe that can differentiate into multiple cell types, including metabolically dormant endospores. We were interested in identifying microbial interactions that affected sporulation in B. subtilis Using a fluorescent transcriptional reporter, we observed that coculturing B. subtilis with Escherichia coli promoted sporulation gene expression via a secreted metabolite. To identify the active compound, we screened the E. coli Keio Collection and identified the sporulation-accelerating cue as the siderophore enterobactin. B. subtilis has multiple iron acquisition systems that are used to take up the B. subtilis- produced siderophore bacillibactin, as well as to pirate exogenous siderophores such as enterobactin. While B. subtilis uses a single substrate binding protein (FeuA) to take up both bacillibactin and enterobactin, we discovered that it requires two distinct genes to sporulate in response to these siderophores (the esterase gene besA for bacillibactin and a putative esterase gene, ybbA , for enterobactin). In addition, we found that siderophores from a variety of other microbial species also promote sporulation in B. subtilis Our results thus demonstrate that siderophores can act not only as bacterial iron acquisition systems but also as interspecies cues that alter cellular development and accelerate sporulation in B. subtilis IMPORTANCE While much is known about the genetic regulation of Bacillus subtilis sporulation, little is understood about how other bacteria influence this process. This work describes an interaction between Escherichia coli and B. subtilis that accelerates sporulation in B. subtilis The interaction is mediated by the E

  9. 4th openlab Board of Sponsors Meeting takes place at CERN on July 6, 2005

    CERN Multimedia

    Patrice Loïez

    2005-01-01

    The 4th openlab Board of Sponsors Meeting is taking place at CERN, room 513, 1-024, the 6th July 2005. The meeting will open with an Executive Session in the presence of Dr. Robert Aymar, Director General of CERN

  10. Asexual sporulation facilitates adaptation

    NARCIS (Netherlands)

    Zhang, Jianhua; Debets, A.J.M.; Verweij, P.E.; Melchers, W.J.G.; Zwaan, B.J.; Schoustra, S.E.

    2015-01-01

    Understanding the occurrence and spread of azole resistance in Aspergillus fumigatus is crucial for public health. It has been hypothesized that asexual sporulation, which is abundant in nature, is essential for phenotypic expression of azole resistance mutations in A. fumigatus facilitating

  11. Waste Disposal: Processes Taking Place (on the way) from the Repository to the Biosphere

    International Nuclear Information System (INIS)

    Put, M.

    2000-01-01

    The main objective of SCK-CEN's R and D programme on the processes taking place on the way from the repository to the biosphere is to provide reliable and defensible models and parameters on the migration of dissolved radionuclides and gases through the host formation (Boom Clay) and the backfill materials of a deep geological repository for high level radioactive waste. The programme and main achievements in this topical area in 1999 are summarised

  12. Diffusion mechanisms taking place at the early stages of cobalt deposition on Au(111)

    International Nuclear Information System (INIS)

    Oviedo, O A; Leiva, E P M; Mariscal, M M

    2008-01-01

    In the present work a detailed atomic-level analysis of some of the main diffusion mechanisms which take place during cobalt adatom deposition are studied within atom dynamics (AD) and the nudged elastic band (NEB) method. Our computer simulations reveal a very fast exchange between Co and Au atoms when the deposit is a single cobalt adatom. However, when the nucleus size increases, a decrease in the exchange probability is observed. Activation energies for different transitions are obtained using AD in combination with the NEB method

  13. DETERMINING THE FEATURES OF SPORTSWEAR TAKING PLACE IN FAST FASHION COLLECTIONS

    Directory of Open Access Journals (Sweden)

    Birsen ÇİLEROĞLU

    2014-07-01

    Full Text Available Spor ts occupies the first place among most siginificant factors increasing quality of life . It has b ecome difficult to allocate proper time for sports in the course of heavy work pace and flow of life . Such circumstances have led people to increase minor sport activities which could be done during short times allocated from daily living, thus, orienting people‟s clothing preference towards sportswear . The feeling of easiness and comfort sportswear offer to individuals enhances further such preference . The feeling of comfort individuals feel in their clothing depends on the presence of physiologic and psy chologic coherence between their bodies and environment . Demand for sportswear allowing easy - movement increased upon rise in life dynamism and standards, it began to be preferred regarding comfort of use and to take its place in daily clothing, too, define d as “casual” clothing . Spor tswear being preferred very much ; has caused the firms making and producing fashion and clothing design to give place in their collections to sportswear category . Particularly, in firms where model and clothing varieties are pl enty and new model design is made in short intervals, named as, “fast fashion” , tendency towards sportswear is growing increasingly . The sale rates of sportswear, utilization rates of which are growing increasingly, has maximum value among total clothing s ales in E - business field, too. In this research, it has been aimed to determine the features of sportswear taking place in “fast fashion” clothing collections . In order to accomplish this aim, 2014 collections of four different brands taking place in natio nal and international markets have been examined through visual analysis method . In the examinations; sportswear styles of the brand, model and style differences between brands and states of using 2014 fashion trends have been taken into account. The data obtained at the end of the analyses made have been

  14. [Marketing approval and market surveillance of medical devices in Germany: Where does policy integration take place?].

    Science.gov (United States)

    Lang, Achim

    2014-01-01

    Since 2011 new regulatory measures regarding medical devices have been set up with the aim to eliminate obstacles to innovations and to find more coordinated ways to marketing authorisation and market surveillance. This essay investigates whether these new and existing coordination mechanisms build up to a Joined-up Government approach. The analysis shows that the regulatory process should be adjusted along several dimensions. First, many organisations lack awareness regarding their stakeholders and focus solely on their immediate organisational activities. Second, the regulatory process (marketing authorisation and market surveillance) is too fragmented for an effective communication to take place. Finally, the underlying strategy process is an ad-hoc approach lacking continuity and continued involvement of, in particular, the responsible federal ministries. Copyright © 2013. Published by Elsevier GmbH.

  15. Inducible Expression of spo0A as a Universal Tool for Studying Sporulation in Clostridium difficile.

    Science.gov (United States)

    Dembek, Marcin; Willing, Stephanie E; Hong, Huynh A; Hosseini, Siamand; Salgado, Paula S; Cutting, Simon M

    2017-01-01

    Clostridium difficile remains a leading nosocomial pathogen, putting considerable strain on the healthcare system. The ability to form endospores, highly resistant to environmental insults, is key to its persistence and transmission. However, important differences exist between the sporulation pathways of C. difficile and the model Gram-positive organism Bacillus subtilis . Amongst the challenges in studying sporulation in C. difficile is the relatively poor levels of sporulation and high heterogeneity in the sporulation process. To overcome these limitations we placed P tet regulatory elements upstream of the master regulator of sporulation, spo0A , generating a new strain that can be artificially induced to sporulate by addition of anhydrotetracycline (ATc). We demonstrate that this strain is asporogenous in the absence of ATc, and that ATc can be used to drive faster and more efficient sporulation. Induction of Spo0A is titratable and this can be used in the study of the spo0A regulon both in vitro and in vivo , as demonstrated using a mouse model of C. difficile infection (CDI). Insights into differences between the sporulation pathways in B. subtilis and C. difficile gained by study of the inducible strain are discussed, further highlighting the universal interest of this tool. The P tet -spo0A strain provides a useful background in which to generate mutations in genes involved in sporulation, therefore providing an exciting new tool to unravel key aspects of sporulation in C. difficile.

  16. Inducible Expression of spo0A as a Universal Tool for Studying Sporulation in Clostridium difficile

    Directory of Open Access Journals (Sweden)

    Marcin Dembek

    2017-09-01

    Full Text Available Clostridium difficile remains a leading nosocomial pathogen, putting considerable strain on the healthcare system. The ability to form endospores, highly resistant to environmental insults, is key to its persistence and transmission. However, important differences exist between the sporulation pathways of C. difficile and the model Gram-positive organism Bacillus subtilis. Amongst the challenges in studying sporulation in C. difficile is the relatively poor levels of sporulation and high heterogeneity in the sporulation process. To overcome these limitations we placed Ptet regulatory elements upstream of the master regulator of sporulation, spo0A, generating a new strain that can be artificially induced to sporulate by addition of anhydrotetracycline (ATc. We demonstrate that this strain is asporogenous in the absence of ATc, and that ATc can be used to drive faster and more efficient sporulation. Induction of Spo0A is titratable and this can be used in the study of the spo0A regulon both in vitro and in vivo, as demonstrated using a mouse model of C. difficile infection (CDI. Insights into differences between the sporulation pathways in B. subtilis and C. difficile gained by study of the inducible strain are discussed, further highlighting the universal interest of this tool. The Ptet-spo0A strain provides a useful background in which to generate mutations in genes involved in sporulation, therefore providing an exciting new tool to unravel key aspects of sporulation in C. difficile.

  17. Noise in a phosphorelay drives stochastic entry into sporulation in Bacillus subtilis.

    Science.gov (United States)

    Russell, Jonathan R; Cabeen, Matthew T; Wiggins, Paul A; Paulsson, Johan; Losick, Richard

    2017-10-02

    Entry into sporulation in Bacillus subtilis is governed by a phosphorelay in which phosphoryl groups from a histidine kinase are successively transferred via relay proteins to the response regulator Spo0A. Spo0A~P, in turn, sets in motion events that lead to asymmetric division and activation of the cell-specific transcription factor σ F , a hallmark for entry into sporulation. Here, we have used a microfluidics-based platform to investigate the activation of Spo0A and σ F in individual cells held under constant, sporulation-inducing conditions. The principal conclusions were that: (i) activation of σ F occurs with an approximately constant probability after adaptation to conditions of nutrient limitation; (ii) activation of σ F is tightly correlated with, and preceded by, Spo0A~P reaching a high threshold level; (iii) activation of Spo0A takes place abruptly just prior to asymmetric division; and (iv) the primary source of noise in the activation of Spo0A is the phosphorelay. We propose that cells exhibit a constant probability of attaining a high threshold level of Spo0A~P due to fluctuations in the flux of phosphoryl groups through the phosphorelay. © 2017 The Authors.

  18. What it Takes to Successfully Implement Technology for Aging in Place: Focus Groups With Stakeholders.

    Science.gov (United States)

    Peek, Sebastiaan Theodorus Michaël; Wouters, Eveline J M; Luijkx, Katrien G; Vrijhoef, Hubertus J M

    2016-05-03

    There is a growing interest in empowering older adults to age in place by deploying various types of technology (ie, eHealth, ambient assisted living technology, smart home technology, and gerontechnology). However, initiatives aimed at implementing these technologies are complicated by the fact that multiple stakeholder groups are involved. Goals and motives of stakeholders may not always be transparent or aligned, yet research on convergent and divergent positions of stakeholders is scarce. To provide insight into the positions of stakeholder groups involved in the implementation of technology for aging in place by answering the following questions: What kind of technology do stakeholders see as relevant? What do stakeholders aim to achieve by implementing technology? What is needed to achieve successful implementations? Mono-disciplinary focus groups were conducted with participants (n=29) representing five groups of stakeholders: older adults (6/29, 21%), care professionals (7/29, 24%), managers within home care or social work organizations (5/29, 17%), technology designers and suppliers (6/29, 21%), and policy makers (5/29, 17%). Transcripts were analyzed using thematic analysis. Stakeholders considered 26 different types of technologies to be relevant for enabling independent living. Only 6 out of 26 (23%) types of technology were mentioned by all stakeholder groups. Care professionals mentioned fewer different types of technology than other groups. All stakeholder groups felt that the implementation of technology for aging in place can be considered a success when (1) older adults' needs and wishes are prioritized during development and deployment of the technology, (2) the technology is accepted by older adults, (3) the technology provides benefits to older adults, and (4) favorable prerequisites for the use of technology by older adults exist. While stakeholders seemed to have identical aims, several underlying differences emerged, for example, with regard

  19. Model of Wagons’ Placing-In and Taking-Out Problem in a Railway Station and Its Heuristic Algorithm

    Directory of Open Access Journals (Sweden)

    Chuijiang Guo

    2014-01-01

    Full Text Available Placing-in and taking-out wagons timely can decrease wagons’ dwell time in railway stations, improve the efficiency of railway transportation, and reduce the cost of goods transportation. We took the locomotive running times between goods operation sites as weights, so the wagons’ placing-in and taking-out problem could be regarded as a single machine scheduling problem, 1pijCmax, which could be transformed into the shortest circle problem in a Hamilton graph whose relaxation problem was an assignment problem. We used a Hungarian algorithm to calculate the optimal solution of the assignment problem. Then we applied a broken circle and connection method, whose computational complexity was O(n2, to find the available satisfactory order of wagons’ placing-in and taking-out. Complex problems, such as placing-in and transferring combined, taking-out and transferring combined, placing-in and taking-out combined, or placing-in, transferring, and taking-out combined, could also be resolved with the extended algorithm. A representative instance was given to illustrate the reliability and efficiency of our results.

  20. SpoVT: From Fine-Tuning Regulator in Bacillus subtilis to Essential Sporulation Protein in Bacillus cereus.

    Science.gov (United States)

    Eijlander, Robyn T; Holsappel, Siger; de Jong, Anne; Ghosh, Abhinaba; Christie, Graham; Kuipers, Oscar P

    2016-01-01

    Sporulation is a highly sophisticated developmental process adopted by most Bacilli as a survival strategy to withstand extreme conditions that normally do not support microbial growth. A complicated regulatory cascade, divided into various stages and taking place in two different compartments of the cell, involves a number of primary and secondary regulator proteins that drive gene expression directed toward the formation and maturation of an endospore. Such regulator proteins are highly conserved among various spore formers. Despite this conservation, both regulatory and phenotypic differences are observed between different species of spore forming bacteria. In this study, we demonstrate that deletion of the regulatory sporulation protein SpoVT results in a severe sporulation defect in Bacillus cereus , whereas this is not observed in Bacillus subtilis . Although spores are initially formed, the process is stalled at a later stage in development, followed by lysis of the forespore and the mother cell. A transcriptomic investigation of B. cereus Δ spoVT shows upregulation of genes involved in germination, potentially leading to premature lysis of prespores formed. Additionally, extreme variation in the expression of species-specific genes of unknown function was observed. Introduction of the B. subtilis SpoVT protein could partly restore the sporulation defect in the B. cereus spoVT mutant strain. The difference in phenotype is thus more than likely explained by differences in promoter targets rather than differences in mode of action of the conserved SpoVT regulator protein. This study stresses that evolutionary variances in regulon members of sporulation regulators can have profound effects on the spore developmental process and that mere protein homology is not a foolproof predictor of similar phenotypes.

  1. Taking back place-names – from dusty library to digital life

    DEFF Research Database (Denmark)

    Knudsen, Bo Nissen

    Danish place-names have been under publication since 1922 in the scientific series Danmarks Stednavne (Place-Names of Denmark) but only recently the huge project of a digitization of the series has been undertaken. Around 120,000 name articles are now on their way to the web as part of the Digital...... atlas of the Danish historical-administrative geography. Digitization and presentation of a scientific place-names edition poses many interesting problems in itself, especially regarding the variation over time in both the selection of names and the build-up of scholarly knowledge. How are we to convey...... mobility of the book format into a digital context – by making the content available as an application for mobile devices such as smart phones and iPads? Adding geocodes to the name articles could open up the possibility of a digital place-name lexicon allowing the end user to move around in a place...

  2. DSC studies of retrogradation and amylose-lipid transition taking place in gamma-irradiated wheat starch

    International Nuclear Information System (INIS)

    Ciesla, K.; Gluszewski, W.; Eliasson, A.C.

    2006-01-01

    It has been already shown that degradation resulting from gamma irradiation induces a decrease in order of starch granules and influences gelatinisation taking place during heating of starch and flour suspensions. In presented paper, DSC (differential scanning calorimetry) studies were carried out for wheat starch, non-irradiated and irradiated using doses in the range from 5 to 30 kGy. The influence of the conditions applied during DSC measurements on the possibility to observe differences between the amylose-lipid complex transition and retrogradation taking place in the non-irradiated and particularly irradiated starch samples was checked. The better differentiation between the amylose-lipid complex transition taking place in particular samples accompanied by the better reproducity were obtained in the case of dense suspensions as compared to the watery suspensions as well as during the first analysis performed for the recrystallised gels

  3. What it takes to successfully implement technology for aging in place: focus groups with stakeholders

    NARCIS (Netherlands)

    Eveline J.M. Wouters; Katrien G. Luijkx; Hubertus J.M. Vrijhoef; Sebastiaan Theodorus Michaël Peek MSc

    2016-01-01

    BACKGROUND: There is a growing interest in empowering older adults to age in place by deploying various types of technology (ie, eHealth, ambient assisted living technology, smart home technology, and gerontechnology). However, initiatives aimed at implementing these technologies are complicated by

  4. Advice from Rural Elders: What It Takes to Age in Place

    Science.gov (United States)

    Dye, Cheryl J.; Willoughby, Deborah F.; Battisto, Dina G.

    2011-01-01

    Older adults prefer to age in place (AIP), and there are psychological, physiological, and economic benefits in doing so. However, it is especially challenging to AIP in rural communities. AIP models have been tested in urban settings and age-segregated communities, but they are not appropriate for rural communities. This paper presents rural AIP…

  5. Clostridium perfringens Sporulation and Sporulation-Associated Toxin Production

    Science.gov (United States)

    Li, Jihong; Paredes-Sabja, Daniel; Sarker, Mahfuzur R.; McClane, Bruce A.

    2015-01-01

    The ability of Clostridium perfringens to form spores plays a key role during the transmission of this Gram-positive bacterium to cause disease. Of particular note, the spores produced by food poisoning strains are often exceptionally resistant to food environment stresses such as heat, cold and preservatives, which likely facilitates their survival in temperature-abused foods. The exceptional resistance properties of spores made by most type A food poisoning strains and some type C foodborne disease strains involves their production of a variant small acid soluble protein-4 that binds more tightly to spore DNA compared to the small acid soluble protein-4 made by most other C. perfringens strains. Sporulation and germination by C. perfringens and Bacillus spp. share both similarities and differences. Finally, sporulation is essential for production of C. perfringens enterotoxin, which is responsible for the symptoms of C. perfringens type A food poisoning, the second most common bacterial foodborne disease in the USA. During this foodborne disease, C. perfringens is ingested with food and then, using sporulation-specific alternate sigma factors, this bacterium sporulates and produces the enterotoxin in the intestines. PMID:27337447

  6. DETERMINING THE FEATURES OF SPORTSWEAR TAKING PLACE IN FAST FASHION COLLECTIONS

    OpenAIRE

    ÇİLEROĞLU, Birsen; KIVILCIMLAR, İnci

    2015-01-01

    Sports occupies the first place among most siginificant factors increasing quality of life. It has become difficult to allocate proper time for sports in the course of heavy work pace and flow of life. Such circumstances have led people to increase minor sport activities which could be done during short times allocated from daily living, thus, orienting people’s clothing preference towards sportswear. The feeling of easiness and comfort sportswear offer to individuals enhances further such pr...

  7. Beyond stereotypes of adolescent risk taking: Placing the adolescent brain in developmental context

    Directory of Open Access Journals (Sweden)

    Daniel Romer

    2017-10-01

    Full Text Available Recent neuroscience models of adolescent brain development attribute the morbidity and mortality of this period to structural and functional imbalances between more fully developed limbic regions that subserve reward and emotion as opposed to those that enable cognitive control. We challenge this interpretation of adolescent development by distinguishing risk-taking that peaks during adolescence (sensation seeking and impulsive action from risk taking that declines monotonically from childhood to adulthood (impulsive choice and other decisions under known risk. Sensation seeking is primarily motivated by exploration of the environment under ambiguous risk contexts, while impulsive action, which is likely to be maladaptive, is more characteristic of a subset of youth with weak control over limbic motivation. Risk taking that declines monotonically from childhood to adulthood occurs primarily under conditions of known risks and reflects increases in executive function as well as aversion to risk based on increases in gist-based reasoning. We propose an alternative Life-span Wisdom Model that highlights the importance of experience gained through exploration during adolescence. We propose, therefore, that brain models that recognize the adaptive roles that cognition and experience play during adolescence provide a more complete and helpful picture of this period of development.

  8. Understanding Where Americas Public Discussion Takes Place In Todays Society: Case Studies of Concealed Weapons Carry Reform

    Science.gov (United States)

    2016-06-01

    arguing that concealed carry permit holders are a danger to public safety and that mass shootings are taking place by citizens who are legally armed.2...who worked at an abortion clinic that had recently been bombed and whose life had been threatened was denied a license to carry because he was not...populace. The new law laid out new prohibitions and penalties enforceable statewide. Additionally, the Preemption Act was necessary to set the legal

  9. Coronavirus and influenza virus proteolytic priming takes place in tetraspanin-enriched membrane microdomains.

    Science.gov (United States)

    Earnest, James T; Hantak, Michael P; Park, Jung-Eun; Gallagher, Tom

    2015-06-01

    Coronaviruses (CoVs) and low-pathogenicity influenza A viruses (LP IAVs) depend on target cell proteases to cleave their viral glycoproteins and prime them for virus-cell membrane fusion. Several proteases cluster into tetraspanin-enriched microdomains (TEMs), suggesting that TEMs are preferred virus entry portals. Here we found that several CoV receptors and virus-priming proteases were indeed present in TEMs. Isolated TEMs, when mixed with CoV and LP IAV pseudoparticles, cleaved viral fusion proteins to fusion-primed fragments and potentiated viral transductions. That entering viruses utilize TEMs as a protease source was further confirmed using tetraspanin antibodies and tetraspanin short hairpin RNAs (shRNAs). Tetraspanin antibodies inhibited CoV and LP IAV infections, but their virus-blocking activities were overcome by expressing excess TEM-associated proteases. Similarly, cells with reduced levels of the tetraspanin CD9 resisted CoV pseudoparticle transductions but were made susceptible by overproducing TEM-associated proteases. These findings indicated that antibodies and CD9 depletions interfere with viral proteolytic priming in ways that are overcome by surplus proteases. TEMs appear to be exploited by some CoVs and LP IAVs for appropriate coengagement with cell receptors and proteases. Enveloped viruses use their surface glycoproteins to catalyze membrane fusion, an essential cell entry step. Host cell components prime these viral surface glycoproteins to catalyze membrane fusion at specific times and places during virus cell entry. Among these priming components are proteases, which cleave viral surface glycoproteins, unleashing them to refold in ways that catalyze virus-cell membrane fusions. For some enveloped viruses, these proteases are known to reside on target cell surfaces. This research focuses on coronavirus and influenza A virus cell entry and identifies TEMs as sites of viral proteolysis, thereby defining subcellular locations of virus

  10. Molecular determinants of sporulation in Ashbya gossypii.

    Science.gov (United States)

    Wasserstrom, Lisa; Lengeler, Klaus B; Walther, Andrea; Wendland, Jürgen

    2013-09-01

    Regulation of development and entry into sporulation is critical for fungi to ensure survival of unfavorable environmental conditions. Here we present an analysis of gene sets regulating sporulation in the homothallic ascomycete Ashbya gossypii. Deletion of components of the conserved pheromone/starvation MAP kinase cascades, e.g., STE11 and STE7, results in increased sporulation. In kar3 mutants sporulation is severely reduced, while deletion of KAR4 as well as of homologs of central Saccharomyces cerevisiae regulators of sporulation, IME1, IME2, IME4, and NDT80, abolishes sporulation in A. gossypii. Comparison of RNAseq transcript profiles of sporulation-deficient mutants identified a set of 67 down-regulated genes, most of which were up-regulated in the oversporulating ste12 mutant. One of these differentially expressed genes is an endoglucanase encoded by ENG2. We found that Eng2p promotes hyphal fragmentation as part of the developmental program of sporulation, which generates single-celled sporangia. Sporulation-deficient strains are arrested in their development but form sporangia. Supply of new nutrients enabled sporangia to return to hyphal growth, indicating that these cells are not locked in meiosis. Double-strand break (DSB) formation by Spo11 is apparently not required for sporulation; however, the absence of DMC1, which repairs DSBs in S. cerevisiae, results in very poor sporulation in A. gossypii. We present a comprehensive analysis of the gene repertoire governing sporulation in A. gossypii and suggest an altered regulation of IME1 expression compared to S. cerevisiae.

  11. Let us play: (unshackling liaisons, (unmasking games and (unhindered dialogue in the arena where theology takes place

    Directory of Open Access Journals (Sweden)

    Tanya van Wyk

    2017-10-01

    Full Text Available This contribution is a political-theological and hermeneutical reflection on the origin, nature, intention and contribution of a research theme identified within the dynamics of an institutional space, by taking a critical look at the �rules� and the �game� of university academia. Specific reference is made to institutional and faculty research themes, namely �reconciling diversity� and �Ecodomy � life in its fullness�. The institutional academic space is compared to a Hunger Games-style panopticon, with its �rules� and �play�. It is argued that these research themes can only make an authentic contribution if the �play� and �game�of the space in which these themes originate, are deconstructed. If this deconstruction can take place, there might be an authentic chance for unhindered dialogue towards the transformation of the academic space and the greater community it serves.

  12. Protein patterns of yeast during sporulation

    International Nuclear Information System (INIS)

    Litske Petersen, J.G.; Kielland-Brandt, M.C.; Nilsson-Tillgren, T.

    1979-01-01

    High resolution two-dimensional gel electrophoresis was used to study protein synthesis during synchronous meiosis and ascospore formation of Saccharomyces cerevisiae. The stained protein patterns of samples harvested at any stage between meiotic prophase and the four-spore stage in two sporulating strains showed the same approximately 250 polypeptides. Of these only a few seemed to increase or decrease in concentration during sporulation. The characteristic pattern of sporulating yeast was identical to the pattern of glucose-grown staitonary yeast cells adapted to respiration. The latter type of cells readily initiates meiosis when transferred to sporulation medium. This pattern differed from the protein patterns of exponentially growing cells in glucose or acetate presporulation medium. Five major proteins in stationary and sporulating yeast cells were not detected in either type of exponential culture. Two-dimensional autoradiograms of [ 35 S]methionine-labelled yeast proteins revealed that some proteins were preferentially labelled during sporulation, while other proteins were labelled at later stages. These patterns differed from the auroradiograms of exponentially growing yeast cells in glucose presporulation medium in a number of spots. No differences were observed when stained gels or autoradiograms of sporulating cultures and non-sporulating strains in sporulation medium were compared. (author)

  13. Spatio-Temporal Evolution of Sporulation in Bacillus thuringiensis Biofilm.

    Science.gov (United States)

    El-Khoury, Nay; Majed, Racha; Perchat, Stéphane; Kallassy, Mireille; Lereclus, Didier; Gohar, Michel

    2016-01-01

    Bacillus thuringiensis can produce a floating biofilm which includes two parts: a ring and a pellicle. The ring is a thick structure which sticks to the culture container, while the pellicle extends over the whole liquid surface and joins the ring. We have followed over time, from 16 to 96 h, sporulation in the two biofilm parts. Sporulation was followed in situ in 48-wells polystyrene microtiterplates with a fluorescence binocular stereomicroscope and a spoIID-yfp transcriptional fusion. Sporulation took place much earlier in the ring than in the pellicle. In 20 h-aged biofilms, spoIID was expressed only in the ring, which could be seen as a green fluorescent circle surrounding the non-fluorescent pellicle. However, after 48 h of culture, the pellicle started to express spoIID in specific area corresponding to protrusions, and after 96 h both the ring and the whole pellicle expressed spoIID. Spore counts and microscopy observations of the ring and the pellicle harvested separately confirmed these results and revealed that sporulation occured 24 h-later in the pellicle comparatively to the ring, although both structures contained nearly 100% spores after 96 h of culture. We hypothesize that two mechanisms, due to microenvironments in the biofilm, can explain this difference. First, the ring experiences a decreased concentration of nutrients earlier than the pellicle, because of a lower exchange area with the culture medium. An second, the ring is exposed to partial dryness. Both reasons could speed up sporulation in this biofilm structure. Our results also suggest that spores in the biofilm display a phenotypic heterogeneity. These observations might be of particular significance for the food industry, since the biofilm part sticking to container walls - the ring - is likely to contain spores and will therefore resist both to washing and to cleaning procedures, and will be able to restart a new biofilm when food production has resumed.

  14. Cell viability and protein turnover in nongrowing Bacillus megaterium at sporulation suppressing temperature.

    Science.gov (United States)

    Kucerová, H; Strnadová, M; Ludvík, J; Chaloupka, J

    1999-01-01

    In Bacillus megaterium, a temperature that suppresses sporulation (43 degrees C) only slightly exceeds both the optimum growth temperature and the temperature still permitting sporulation (40-41 degrees C). Here we show that, when cells grown at 35 degrees C and transferred to a sporulation medium, were subjected to shifts between 35 degrees C and the sporulation suppressing temperature (SST, 43 degrees C), their development and proteolytic activities were deeply affected. During the reversible sporulation phase that took place at 35 degrees C for 2-3 h (T2-T3), the cells developed forespores and their protein turnover was characterized by degradation of short-lived proteins and proteins made accessible to the proteolytic attack because of starvation. During the following irreversible sporulation phase refractile heat-resistant spores appeared at T4-T5. Protein turnover rate increased again after T2 and up to T8 60-70% prelabelled proteins were degraded. The SST suppressed sporulation at its beginning; at T3 no asymmetric septa were observed and the amount of heat-resistant spores at T8 was by 4-5 orders lower than at 35 degrees C. However, the cells remained viable and were able to sporulate when transferred to a lower temperature. Protein degradation was increased up to T3 but then its velocity sharply dropped and the amount of degraded protein at T8 corresponded to slightly more than one-half of that found at 35 degrees C. The cytoplasmic proteolytic activity was enhanced but the activity in the membrane fraction was decreased. When a temperature shift to SST was applied at the beginning of the irreversible sporulation phase (T2.5), the sporulation process was impaired. A portion of forespores lyzed, the others were able to complete their development but most spores were not heat-resistant and their coats showed defects. Protein degradation increased again because an effective proteolytic system was developed during the reversible sporulation phase but the

  15. The albedo problem in the case of multiple synthetic scattering taking place in a plane-symmetric slab

    International Nuclear Information System (INIS)

    Shafiq, A.; Meyer, H.E. de; Grosjean, C.C.

    1985-01-01

    An approximate model based on an improved diffusion-type theory is established for treating multiple synthetic scattering in a homogeneous slab of finite thickness. As in the case of the exact treatment given in the preceding paper (Part I), it appears possible to transform the considered transport problem into an equivalent fictitious one involving multiple isotropic scattering, therefore permitting the application of an established corrected diffusion theory for treating isotropic scattering taking place in a convex homogeneous medium bounded by a vacuum in the presence of various types of sources. The approximate values of the reflection and transmission coefficients are compared with the rigorous values listed in Part I. In this way, the high accuracy of the approximation is clearly demonstrated. (author)

  16. DSC Studies of Retrogradation and Amylose-Lipid Complex Transition Taking Place in Gamma Irradiated Wheat Starch

    International Nuclear Information System (INIS)

    Ciesla, K.

    2006-01-01

    Degradation resulting from gamma irradiation induces decrease in order of starch granules and influences the processes occurring in starch-water system. Differential scanning calorimetry (DSC) was applied at present for studying the effect of radiation with doses of 5 - 30 kGy on amylose-lipid complex transition and retrogradation occurring in wheat starch gels. Influence of the conditions applied during DSC measurements and intermediate storage was tested on the possibility to observe radiation effect. Wheat starch was irradiated with 60 C o gamma rays in a gamma cell Issledovatiel placed in the Department of Radiation Chemistry, INCT. DSC measurements were performed for ca. 50% and ca. 20% gels during heating - cooling - heating cycles (up to 3 cycles) in the temperature range 10 - 150 degree at heating and cooling rates of 10, 5 and 2.5 degree min - 1. The Seiko DSC 6200 calorimeter was used. Decrease in amylose-lipid complex transition temperature was found already after irradiation of wheat starch with a dose of 5 kGy showing modificatin of the complex structure. The differences between the irradiated and the non-irradiated samples became the easier seen in the every foregoing heating or cooling cycle as compared to the preceeding one. It is because that thermal treatment causes decrease of transition temperature in all the irradiated samples, with no effect or increase of that temperature observed in the non-irradiated ones. Irradiation hinders retrogradation taking place in ca. 50% gels but facilitates retrogradation occurring in ca. 20 % gels. Moreover, the expanded differences between the amylose-lipid complex formed in the irradiated and non-irradiated gels result due to their recrystallisation. Storage of the gels induces decrease in the temperature of the complex transition as compared to the last cycle of the first analysis. That decrease was, however, more significant in the case of all the irradiated samples than in the case of the initial sample. In

  17. Sporulation genes associated with sporulation efficiency in natural isolates of yeast.

    Science.gov (United States)

    Tomar, Parul; Bhatia, Aatish; Ramdas, Shweta; Diao, Liyang; Bhanot, Gyan; Sinha, Himanshu

    2013-01-01

    Yeast sporulation efficiency is a quantitative trait and is known to vary among experimental populations and natural isolates. Some studies have uncovered the genetic basis of this variation and have identified the role of sporulation genes (IME1, RME1) and sporulation-associated genes (FKH2, PMS1, RAS2, RSF1, SWS2), as well as non-sporulation pathway genes (MKT1, TAO3) in maintaining this variation. However, these studies have been done mostly in experimental populations. Sporulation is a response to nutrient deprivation. Unlike laboratory strains, natural isolates have likely undergone multiple selections for quick adaptation to varying nutrient conditions. As a result, sporulation efficiency in natural isolates may have different genetic factors contributing to phenotypic variation. Using Saccharomyces cerevisiae strains in the genetically and environmentally diverse SGRP collection, we have identified genetic loci associated with sporulation efficiency variation in a set of sporulation and sporulation-associated genes. Using two independent methods for association mapping and correcting for population structure biases, our analysis identified two linked clusters containing 4 non-synonymous mutations in genes - HOS4, MCK1, SET3, and SPO74. Five regulatory polymorphisms in five genes such as MLS1 and CDC10 were also identified as putative candidates. Our results provide candidate genes contributing to phenotypic variation in the sporulation efficiency of natural isolates of yeast.

  18. Where and How Do Aging Processes Take Place in Everyday Life? Answers From a New Materialist Perspective

    Directory of Open Access Journals (Sweden)

    Grit Höppner

    2018-04-01

    Full Text Available In the last decade, the focus of studies on age and aging has fundamentally changed from biological to symbolic, discursive, and cultural phenomena. Currently, the most studied topic in material gerontology is the materiality of age and aging in the context of everyday life. Scholars in this area have thus been making an important contribution to a material understanding of aging processes. As we understand them, however, both social constructivist and material gerontological concepts reach their limit when it comes to the questions of where and how aging processes actually take place in everyday life. In order to answer these two questions, we review social constructivist ideas with a particular focus on the “doing age” concept and material gerontological assumptions regarding human subjects, their material environments, and their relations. We then suggest rethinking bodily limitations and agencies addressed by scholars in the field of new materialism. The aim is to develop a new materialist-inspired understanding of aging processes that helps to reconstruct the material-discursive co-production of aging processes. These processes are deployed as mutual entanglements of materiality and meaning as well as of humans and non-human agency. This approach emphasizes the decentralization of the human actor and thus helps to map the material-discursive complexity of aging processes as relational co-products of humans and non-humans in everyday life.

  19. Ultrasensitivity of the Bacillus subtilis sporulation decision.

    Science.gov (United States)

    Narula, Jatin; Devi, Seram N; Fujita, Masaya; Igoshin, Oleg A

    2012-12-11

    Starving Bacillus subtilis cells execute a gene expression program resulting in the formation of stress-resistant spores. Sporulation master regulator, Spo0A, is activated by a phosphorelay and controls the expression of a multitude of genes, including the forespore-specific sigma factor σ(F) and the mother cell-specific sigma factor σ(E). Identification of the system-level mechanism of the sporulation decision is hindered by a lack of direct control over Spo0A activity. This limitation can be overcome by using a synthetic system in which Spo0A activation is controlled by inducing expression of phosphorelay kinase KinA. This induction results in a switch-like increase in the number of sporulating cells at a threshold of KinA. Using a combination of mathematical modeling and single-cell microscopy, we investigate the origin and physiological significance of this ultrasensitive threshold. The results indicate that the phosphorelay is unable to achieve a sufficiently fast and ultrasensitive response via its positive feedback architecture, suggesting that the sporulation decision is made downstream. In contrast, activation of σ(F) in the forespore and of σ(E) in the mother cell compartments occurs via a cascade of coherent feed-forward loops, and thereby can produce fast and ultrasensitive responses as a result of KinA induction. Unlike σ(F) activation, σ(E) activation in the mother cell compartment only occurs above the KinA threshold, resulting in completion of sporulation. Thus, ultrasensitive σ(E) activation explains the KinA threshold for sporulation induction. We therefore infer that under uncertain conditions, cells initiate sporulation but postpone making the sporulation decision to average stochastic fluctuations and to achieve a robust population response.

  20. What does it take to make them stay? : how place satisfaction relates to willingness to stay of the creative class

    OpenAIRE

    Annell, Jonas; Terman, Felix

    2017-01-01

    Swedish students in smaller university host cities leave in favor of more attractive places after having completed their studies. Failing to retain newly graduated students is a problem for university host cities as educated people are associated with the group referred to as the creative class. Members of the creative class are drivers of regional economic growth, and have historically proven to be important for creating and developing the well-being of cities. Different place attributes hav...

  1. Catabolite repression of enzyme synthesis does not prevent sporulation.

    OpenAIRE

    Lopez, J M; Uratani-Wong, B; Freese, E

    1980-01-01

    In the presence of excess glucose, a decrease of guanine nucleotides in Bacillus subtilis initiated sporulation but did not prevent catabolite repression of three enzymes. Therefore, the ultimate mechanism(s) repressing enzyme synthesis differs from that suppressing sporulation.

  2. Young People Take Their Rightful Places as Full and Contributing Members of a World Class Workforce: Philadelphia Youth Network Annual Report 2006

    Science.gov (United States)

    Philadelphia Youth Network, 2006

    2006-01-01

    The title of this year's annual report has particular meaning for all of the staff at the Philadelphia Youth Network. The phrase derives from Philadelphia Youth Network's (PYN's) new vision statement, developed as part of its recent strategic planning process, which reads: All of our city's young people take their rightful places as full and…

  3. Stuck between a ROC and a hard place? Barriers to the take up of green energy in the UK

    International Nuclear Information System (INIS)

    Diaz-Rainey, Ivan; Ashton, John K.

    2008-01-01

    This paper examines the UK mechanisms for ensuring future investment in renewable energy through consumer adoption of green energy tariffs and the Renewable Obligation Certificate (ROC) system. Using a national survey and focus groups the stated willingness by UK customers to pay a premium for renewable or green energy and actual take up of such tariffs is assessed. Substantial differences between willingness to pay for and the adoption of green energy tariffs are reported. This disparity is linked to a range of factors including consumer confusion, lack of supply, complexities of constructing 'green source' tariffs under the ROC system and a lack of customer trust. It is concluded that the re-definition of the green energy market in favour of 'green source' tariffs, greater direct compliance with the Renewable Obligation by addressing supply constraints, and efforts in providing clearer information and choices for consumers via a compulsory green energy accreditation scheme are required if willing consumers' are to contribute to investment in renewable energy

  4. CodY-Dependent Regulation of Sporulation in Clostridium difficile.

    Science.gov (United States)

    Nawrocki, Kathryn L; Edwards, Adrianne N; Daou, Nadine; Bouillaut, Laurent; McBride, Shonna M

    2016-08-01

    Clostridium difficile must form a spore to survive outside the gastrointestinal tract. The factors that trigger sporulation in C. difficile remain poorly understood. Previous studies have suggested that a link exists between nutritional status and sporulation initiation in C. difficile In this study, we investigated the impact of the global nutritional regulator CodY on sporulation in C. difficile strains from the historical 012 ribotype and the current epidemic 027 ribotype. Sporulation frequencies were increased in both backgrounds, demonstrating that CodY represses sporulation in C. difficile The 027 codY mutant exhibited a greater increase in spore formation than the 012 codY mutant. To determine the role of CodY in the observed sporulation phenotypes, we examined several factors that are known to influence sporulation in C. difficile Using transcriptional reporter fusions and quantitative reverse transcription-PCR (qRT-PCR) analysis, we found that two loci associated with the initiation of sporulation, opp and sinR, are regulated by CodY. The data demonstrate that CodY is a repressor of sporulation in C. difficile and that the impact of CodY on sporulation and expression of specific genes is significantly influenced by the strain background. These results suggest that the variability of CodY-dependent regulation is an important contributor to virulence and sporulation in current epidemic isolates. This report provides further evidence that nutritional state, virulence, and sporulation are linked in C. difficile This study sought to examine the relationship between nutrition and sporulation in C. difficile by examining the global nutritional regulator CodY. CodY is a known virulence and nutritional regulator of C. difficile, but its role in sporulation was unknown. Here, we demonstrate that CodY is a negative regulator of sporulation in two different ribotypes of C. difficile We also demonstrate that CodY regulates known effectors of sporulation, Opp and Sin

  5. Conserved oligopeptide permeases modulate sporulation initiation in Clostridium difficile.

    Science.gov (United States)

    Edwards, Adrianne N; Nawrocki, Kathryn L; McBride, Shonna M

    2014-10-01

    The anaerobic gastrointestinal pathogen Clostridium difficile must form a metabolically dormant spore to survive in oxygenic environments and be transmitted from host to host. The regulatory factors by which C. difficile initiates and controls the early stages of sporulation in C. difficile are not highly conserved in other Clostridium or Bacillus species. Here, we investigated the role of two conserved oligopeptide permeases, Opp and App, in the regulation of sporulation in C. difficile. These permeases are known to positively affect sporulation in Bacillus species through the import of sporulation-specific quorum-sensing peptides. In contrast to other spore-forming bacteria, we discovered that inactivating these permeases in C. difficile resulted in the earlier expression of early sporulation genes and increased sporulation in vitro. Furthermore, disruption of opp and app resulted in greater virulence and increased the amounts of spores recovered from feces in the hamster model of C. difficile infection. Our data suggest that Opp and App indirectly inhibit sporulation, likely through the activities of the transcriptional regulator SinR and its inhibitor, SinI. Taken together, these results indicate that the Opp and App transporters serve a different function in controlling sporulation and virulence in C. difficile than in Bacillus subtilis and suggest that nutrient availability plays a significant role in pathogenesis and sporulation in vivo. This study suggests a link between the nutritional status of the environment and sporulation initiation in C. difficile. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

  6. The Investigation of Mobbing Events Taking Place at Higher Education Institutions in Turkey Considering the Reflections on Media

    Directory of Open Access Journals (Sweden)

    Çubukçu Zühal

    2015-08-01

    considering that they are published nationally, reference newspapers and belong to different publication groups. The data used in this study was taken from the archive of the three newspapers. When the archives of the newspapers was scanned, the word “mobbing” was taken as key word for the search. In qualitative researches, newspapers are considered to be public archive and used as reference documents. The data obtained was grouped depending on the newspapers which it was taken from, on the year of publication, on where they took place, on the gender of the victims and on who the sufferers are.

  7. Sporulation in the Budding Yeast Saccharomyces cerevisiae

    Science.gov (United States)

    Neiman, Aaron M.

    2011-01-01

    In response to nitrogen starvation in the presence of a poor carbon source, diploid cells of the yeast Saccharomyces cerevisiae undergo meiosis and package the haploid nuclei produced in meiosis into spores. The formation of spores requires an unusual cell division event in which daughter cells are formed within the cytoplasm of the mother cell. This process involves the de novo generation of two different cellular structures: novel membrane compartments within the cell cytoplasm that give rise to the spore plasma membrane and an extensive spore wall that protects the spore from environmental insults. This article summarizes what is known about the molecular mechanisms controlling spore assembly with particular attention to how constitutive cellular functions are modified to create novel behaviors during this developmental process. Key regulatory points on the sporulation pathway are also discussed as well as the possible role of sporulation in the natural ecology of S. cerevisiae. PMID:22084423

  8. Sporulation dynamics of poultry Eimeria oocysts in Chennai.

    Science.gov (United States)

    Venkateswara Rao, P; Raman, M; Gomathinayagam, S

    2015-12-01

    The infective form of Eimeria is the highly resistant oocyst, which is shed in the faeces of infected animals. Present study was carried out to understand the sporulation dynamics of six Eimeria oocysts viz. E. acervulina, E. brunetti, E. maxima, E. mitis, E. necatrix and E. tenella in Chennai. Faecal samples of poultry were collected from various poultry farms located in and around Tamil Nadu. Oocysts of various Eimeria species were examined microscopically for sporulation on a 6 h interval basis till complete sporulation is acheived. The sporulation time recorded was 168, 120, 216, 192, 96 and 96 h for E. acervulina, E. brunetti, E. maxima, E. mitis, E. necatrix and E. tenella respectively. It can be concluded on comparison with previous studies that humid weather conditions delay the sporulation time and dry weather and wet litter is the ideal condition for rapid sporulation.

  9. Identification of new developmentally regulated genes involved in Streptomyces coelicolor sporulation.

    Science.gov (United States)

    Salerno, Paola; Persson, Jessica; Bucca, Giselda; Laing, Emma; Ausmees, Nora; Smith, Colin P; Flärdh, Klas

    2013-12-05

    The sporulation of aerial hyphae of Streptomyces coelicolor is a complex developmental process. Only a limited number of the genes involved in this intriguing morphological differentiation programme are known, including some key regulatory genes. The aim of this study was to expand our knowledge of the gene repertoire involved in S. coelicolor sporulation. We report a DNA microarray-based investigation of developmentally controlled gene expression in S. coelicolor. By comparing global transcription patterns of the wild-type parent and two mutants lacking key regulators of aerial hyphal sporulation, we found a total of 114 genes that had significantly different expression in at least one of the two mutants compared to the wild-type during sporulation. A whiA mutant showed the largest effects on gene expression, while only a few genes were specifically affected by whiH mutation. Seven new sporulation loci were investigated in more detail with respect to expression patterns and mutant phenotypes. These included SCO7449-7451 that affect spore pigment biogenesis; SCO1773-1774 that encode an L-alanine dehydrogenase and a regulator-like protein and are required for maturation of spores; SCO3857 that encodes a protein highly similar to a nosiheptide resistance regulator and affects spore maturation; and four additional loci (SCO4421, SCO4157, SCO0934, SCO1195) that show developmental regulation but no overt mutant phenotype. Furthermore, we describe a new promoter-probe vector that takes advantage of the red fluorescent protein mCherry as a reporter of cell type-specific promoter activity. Aerial hyphal sporulation in S. coelicolor is a technically challenging process for global transcriptomic investigations since it occurs only as a small fraction of the colony biomass and is not highly synchronized. Here we show that by comparing a wild-type to mutants lacking regulators that are specifically affecting processes in aerial hypha, it is possible to identify previously

  10. Effects of CO/sub 2/ and membranes on sporulation in axenic cultures of flax rust. [Melampsora lini

    Energy Technology Data Exchange (ETDEWEB)

    Boasson, R.; Shaw, M.

    1985-01-01

    Uredospore production by axenically grown flax rust (Melampsora lini (Ehrenb.) Lev.) was measured as carotenoids (extinction units at 458 nm) per milligram protein. Sporulation was not affected by raising (flushing with 1-5% (v/v) CO/sub 2/ in air) or lowering (KOH well in culture flasks) the level of CO/sub 2/ in the air space above the cultures. Significant (two- to four-fold) increases in sporulation occurred beneath impermeable membranes of parafilm or Saran wrap placed on the surface of young (3 weeks from seeding) mycelial mats for 2 weeks. The stimulatory effect was confined strictly to those areas of the mycelial mats in contact with the membranes. Both Parafilm and Saran wrap were easily and cleanly peeled away from the mycelial mats. Permeable Unipore and HVHP membranes, to which the fungus adhered strongly, did not stimulate sporulation. The fungus did not adhere to Unipore or HVHP membranes treated with silicone or paraffin oil; membranes thus treated stimulated sporulation. The stimulatory effect of membranes on sporulation appears to depend on the nature of the contact between the membrane surface and the mycelium and to be unrelated to the effect of the membranes on the diffusion of gases or other volatile substances. 11 references, 2 figures, 4 tables.

  11. A derepression system based on the Bacillus subtilis sporulation pathway offers dynamic control of heterologous gene expression

    NARCIS (Netherlands)

    Nijland, Reindert; Veening, Jan-Willem; Kuipers, Oscar P.

    By rewiring the sporulation gene-regulatory network of Bacillus subtilis, we generated a novel expression system relying on derepression. The gene of interest is placed under the control of the abrB promoter, which is active only when Spo0A is absent, and Spo0A is controlled via an IPTG

  12. Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format.

    Science.gov (United States)

    Paulissen, Scott M; Huang, Linda S

    2016-09-17

    During times of nutritional stress, Saccharomyces cerevisiae undergoes gametogenesis, known as sporulation. Diploid yeast cells that are starved for nitrogen and carbon will initiate the sporulation process. The process of sporulation includes meiosis followed by spore formation, where the haploid nuclei are packaged into environmentally resistant spores. We have developed methods for the efficient sporulation of budding yeast in 96 multiwell plates, to increase the throughput of screening yeast cells for sporulation phenotypes. These methods are compatible with screening with yeast containing plasmids requiring nutritional selection, when appropriate minimal media is used, or with screening yeast with genomic alterations, when a rich presporulation regimen is used. We find that for this method, aeration during sporulation is critical for spore formation, and have devised techniques to ensure sufficient aeration that are compatible with the 96 multiwell plate format. Although these methods do not achieve the typical ~80% level of sporulation that can be achieved in large-volume flask based experiments, these methods will reliably achieve about 50-60% level of sporulation in small-volume multiwell plates.

  13. Sporulation during growth in a gut isolate of Bacillus subtilis.

    Science.gov (United States)

    Serra, Cláudia R; Earl, Ashlee M; Barbosa, Teresa M; Kolter, Roberto; Henriques, Adriano O

    2014-12-01

    Sporulation by Bacillus subtilis is a cell density-dependent response to nutrient deprivation. Central to the decision of entering sporulation is a phosphorelay, through which sensor kinases promote phosphorylation of Spo0A. The phosphorelay integrates both positive and negative signals, ensuring that sporulation, a time- and energy-consuming process that may bring an ecological cost, is only triggered should other adaptations fail. Here we report that a gastrointestinal isolate of B. subtilis sporulates with high efficiency during growth, bypassing the cell density, nutritional, and other signals that normally make sporulation a post-exponential-phase response. Sporulation during growth occurs because Spo0A is more active per cell and in a higher fraction of the population than in a laboratory strain. This in turn, is primarily caused by the absence from the gut strain of the genes rapE and rapK, coding for two aspartyl phosphatases that negatively modulate the flow of phosphoryl groups to Spo0A. We show, in line with recent results, that activation of Spo0A through the phosphorelay is the limiting step for sporulation initiation in the gut strain. Our results further suggest that the phosphorelay is tuned to favor sporulation during growth in gastrointestinal B. subtilis isolates, presumably as a form of survival and/or propagation in the gut environment. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

  14. Incorporation of glycine and serine into sporulating cells of Bacillus subtilis

    International Nuclear Information System (INIS)

    Mitani, Takahiko; Kadota, Hajime

    1976-01-01

    The changes during growth and sporulation in activities of cells of Bacillus subtilis to incorporate various amino acids were investigated with wild-type strain and its asporogenous mutant. In the case of wild type strain the uptake of valine, phenylalanine, and proline was largest during the logarithmic growth period. The uptake of these amino acids decreased rapidly during the early stationary phase. The uptake of valine and cysteine increased again to some extent just prior to the forespore stage. The uptake of glycine and serine, however, was largest at the forespore stage at which the formation of spore coat took place. From these observed phenomena it was assumed that the remarkable incorporation of glycine and serine into the wild type strain during sporulation was closely related to the formation of spore coat. (auth.)

  15. Genomic determinants of sporulation in Bacilli and Clostridia: towards the minimal set of sporulation-specific genes.

    Science.gov (United States)

    Galperin, Michael Y; Mekhedov, Sergei L; Puigbo, Pere; Smirnov, Sergey; Wolf, Yuri I; Rigden, Daniel J

    2012-11-01

    Three classes of low-G+C Gram-positive bacteria (Firmicutes), Bacilli, Clostridia and Negativicutes, include numerous members that are capable of producing heat-resistant endospores. Spore-forming firmicutes include many environmentally important organisms, such as insect pathogens and cellulose-degrading industrial strains, as well as human pathogens responsible for such diseases as anthrax, botulism, gas gangrene and tetanus. In the best-studied model organism Bacillus subtilis, sporulation involves over 500 genes, many of which are conserved among other bacilli and clostridia. This work aimed to define the genomic requirements for sporulation through an analysis of the presence of sporulation genes in various firmicutes, including those with smaller genomes than B. subtilis. Cultivable spore-formers were found to have genomes larger than 2300 kb and encompass over 2150 protein-coding genes of which 60 are orthologues of genes that are apparently essential for sporulation in B. subtilis. Clostridial spore-formers lack, among others, spoIIB, sda, spoVID and safA genes and have non-orthologous displacements of spoIIQ and spoIVFA, suggesting substantial differences between bacilli and clostridia in the engulfment and spore coat formation steps. Many B. subtilis sporulation genes, particularly those encoding small acid-soluble spore proteins and spore coat proteins, were found only in the family Bacillaceae, or even in a subset of Bacillus spp. Phylogenetic profiles of sporulation genes, compiled in this work, confirm the presence of a common sporulation gene core, but also illuminate the diversity of the sporulation processes within various lineages. These profiles should help further experimental studies of uncharacterized widespread sporulation genes, which would ultimately allow delineation of the minimal set(s) of sporulation-specific genes in Bacilli and Clostridia. Published 2012. This article is a U.S. Government work and is in the public domain in the USA.

  16. Sporulation of Clostridium cylindrosporum on a Defined, Low-Manganese Medium

    OpenAIRE

    Sacks, L. E.; Smith, M. R.

    1987-01-01

    Clostridium cylindrosporum HC-1 grew and sporulated well on a defined medium. This is the first demonstration of sporulation of a purinolytic clostridium on a defined medium; manganese levels were below those considered essential for sporulation of most Bacillus species. Sporulation appeared to be initiated before exhaustion of the purine substrate.

  17. Phosphate limitation induces sporulation in the chytridiomycete Blastocladiella emersonii.

    Science.gov (United States)

    Bongiorno, Vagner Alexandre; Ferreira da Cruz, Angela; Nunis da Silva, Antonio; Corrêa, Luiz Carlos

    2012-09-01

    The cell cycle is controlled by numerous mechanisms that ensure correct cell division. If growth is not possible, cells may eventually promote autophagy, differentiation, or apoptosis. Microorganisms interrupt their growth and differentiate under general nutrient limitation. We analyzed the effects of phosphate limitation on growth and sporulation in the chytridiomycete Blastocladiella emersonii using kinetic data, phase-contrast, and laser confocal microscopy. Under phosphate limitation, zoospores germinated and subsequently formed 2-4 spores, regardless of the nutritional content of the medium. The removal of phosphate at any time during growth induced sporulation of vegetative cells. If phosphate was later added to the same cultures, growth was restored if the cells were not yet committed to sporulation. The cycles of addition and withdrawal of phosphate from growth medium resulted in cycles of germination-growth, germination-sporulation, or germination-growth-sporulation. These results show that phosphate limitation is sufficient to interrupt cell growth and to induce complete sporulation in B. emersonii. We concluded that the determination of growth or sporulation in this microorganism is linked to phosphate availability when other nutrients are not limiting. This result provides a new tool for the dissection of nutrient-energy and signal pathways in cell growth and differentiation.

  18. When and Where Learning is Taking Place: Multisynaptic Changes in Strength During Different Behaviors Related to the Acquisition of an Operant Conditioning Task by Behaving Rats.

    Science.gov (United States)

    Fernández-Lamo, Iván; Delgado-García, José M; Gruart, Agnès

    2018-03-01

    Although it is generally assumed that brain circuits are modified by new experiences, the question of which changes in synaptic efficacy take place in cortical and subcortical circuits across the learning process remains unanswered. Rats were trained in the acquisition of an operant conditioning in a Skinner box provided with light beams to detect animals' approaches to lever and feeder. Behaviors such as pressing the lever, eating, exploring, and grooming were also recorded. Animals were chronically implanted with stimulating and recording electrodes in hippocampal, prefrontal, and subcortical sites relevant to the task. Field synaptic potentials were evoked during the performance of the above-mentioned behaviors and before, during, and after the acquisition process. Afferent pathways to the hippocampus and the intrinsic hippocampal circuit were slightly modified in synaptic strength during the performance of those behaviors. In contrast, afferent and efferent circuits of the medial prefrontal cortex were significantly modified in synaptic strength across training sessions, mostly at the moment of the largest change in the learning curve. Performance of behaviors nondirectly related to the acquisition process (exploring, grooming) also evoked changes in synaptic strength across training. This study helps to understand when and where learning is being engraved in the brain. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  19. The Clostridium sporulation programs: diversity and preservation of endospore differentiation.

    Science.gov (United States)

    Al-Hinai, Mohab A; Jones, Shawn W; Papoutsakis, Eleftherios T

    2015-03-01

    Bacillus and Clostridium organisms initiate the sporulation process when unfavorable conditions are detected. The sporulation process is a carefully orchestrated cascade of events at both the transcriptional and posttranslational levels involving a multitude of sigma factors, transcription factors, proteases, and phosphatases. Like Bacillus genomes, sequenced Clostridium genomes contain genes for all major sporulation-specific transcription and sigma factors (spo0A, sigH, sigF, sigE, sigG, and sigK) that orchestrate the sporulation program. However, recent studies have shown that there are substantial differences in the sporulation programs between the two genera as well as among different Clostridium species. First, in the absence of a Bacillus-like phosphorelay system, activation of Spo0A in Clostridium organisms is carried out by a number of orphan histidine kinases. Second, downstream of Spo0A, the transcriptional and posttranslational regulation of the canonical set of four sporulation-specific sigma factors (σ(F), σ(E), σ(G), and σ(K)) display different patterns, not only compared to Bacillus but also among Clostridium organisms. Finally, recent studies demonstrated that σ(K), the last sigma factor to be activated according to the Bacillus subtilis model, is involved in the very early stages of sporulation in Clostridium acetobutylicum, C. perfringens, and C. botulinum as well as in the very late stages of spore maturation in C. acetobutylicum. Despite profound differences in initiation, propagation, and orchestration of expression of spore morphogenetic components, these findings demonstrate not only the robustness of the endospore sporulation program but also the plasticity of the program to generate different complex phenotypes, some apparently regulated at the epigenetic level. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  20. Updates on the sporulation process in Clostridium species.

    Science.gov (United States)

    Talukdar, Prabhat K; Olguín-Araneda, Valeria; Alnoman, Maryam; Paredes-Sabja, Daniel; Sarker, Mahfuzur R

    2015-05-01

    Sporulation is an important strategy for certain bacterial species within the phylum Firmicutes to survive longer periods of time in adverse conditions. All spore-forming bacteria have two phases in their life; the vegetative form, where they can maintain all metabolic activities and replicate to increase numbers, and the spore form, where no metabolic activities exist. Although many essential components of sporulation are conserved among the spore-forming bacteria, there are differences in the regulation and the pathways among different genera, even at the species level. While we have gained much information from the most studied spore-forming bacterial genus, Bacillus, we still lack an in-depth understanding of spore formation in the genus Clostridium. Clostridium and Bacillus share the master regulator of sporulation, Spo0A, and its downstream pathways, but there are differences in the activation of the Spo0A pathway. While Bacillus species use a multi-component phosphorylation pathway for phosphorylation of Spo0A, termed phosphorelay, such a phosphorelay system is absent in Clostridium. On the other hand, a number of genes regulated by the different sporulation-specific transcription factors are conserved between different Clostridium and Bacillus species. In this review, we discuss the recent findings on Clostridium sporulation and compare the sporulation mechanism in Clostridium and Bacillus. Copyright © 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

  1. Thin Places

    OpenAIRE

    Lockwood, Sandra Elizabeth

    2013-01-01

    This inquiry into the three great quests of the twentieth century–the South Pole, Mount Everest, and the Moon–examines our motivations to venture into these sublime, yet life-taking places. The Thin Place was once the destination of the religious pilgrim seeking transcendence in an extreme environment. In our age, the Thin Place quest has morphed into a challenge to evolve beyond the confines of our own physiology; through human ingenuity and invention, we reach places not meant to accommod...

  2. Chromosomal Arrangement of Phosphorelay Genes Couples Sporulation and DNA Replication.

    Science.gov (United States)

    Narula, Jatin; Kuchina, Anna; Lee, Dong-Yeon D; Fujita, Masaya; Süel, Gürol M; Igoshin, Oleg A

    2015-07-16

    Genes encoding proteins in a common regulatory network are frequently located close to one another on the chromosome to facilitate co-regulation or couple gene expression to growth rate. Contrasting with these observations, here, we demonstrate a functional role for the arrangement of Bacillus subtilis sporulation network genes on opposite sides of the chromosome. We show that the arrangement of two sporulation network genes, one located close to the origin and the other close to the terminus, leads to a transient gene dosage imbalance during chromosome replication. This imbalance is detected by the sporulation network to produce cell-cycle coordinated pulses of the sporulation master regulator Spo0A∼P. This pulsed response allows cells to decide between sporulation and continued vegetative growth during each cell cycle spent in starvation. The simplicity of this coordination mechanism suggests that it may be widely applicable in a variety of gene regulatory and stress-response settings. VIDEO ABSTRACT. Copyright © 2015 Elsevier Inc. All rights reserved.

  3. Cell-density-dependent lysis and sporulation of Myxococcus xanthus in agarose microbeads.

    OpenAIRE

    Rosenbluh, A; Nir, R; Sahar, E; Rosenberg, E

    1989-01-01

    Vegetative cells of Myxococcus xanthus were immobilized in 25-microns-diameter agarose microbeads and incubated in either growth medium or sporulation buffer. In growth medium, the cells multiplied, glided to the periphery, and then filled the beads. In sporulation buffer, up to 90% of the cells lysed and ca. 50% of the surviving cells formed resistant spores. A strong correlation between sporulation and cell lysis was observed; both phenomena were cell density dependent. Sporulation proficie...

  4. Proteins that interact with GTP during sporulation of Bacillus subtilis

    International Nuclear Information System (INIS)

    Mitchell, C.; Vary, J.C.

    1989-01-01

    During sporulation of Bacillus subtilis, several proteins were shown to interact with GTP in specific ways. UV light was used to cross-link [α- 32 P]GTP to proteins in cell extracts at different stages of growth. After electrophoresis, 11 bands of radioactivity were found in vegetative cells, 4 more appeared during sporulation, and only 9 remained in mature spores. Based on the labeling pattern with or without UV light to cross-link either [α- 32 P]GTP or [γ- 32 P]GTP, 11 bands of radioactivity were apparent guanine nucleotide-binding proteins, and 5 bands appeared to be phosphorylated and/or guanylated. Similar results were found with Bacillus megaterium. Assuming the GTP might be a type of signal for sporulation, it could interact with and regulate proteins by at least three mechanisms

  5. Effects of Artemisia annua extracts on sporulation of Eimeria oocysts.

    Science.gov (United States)

    Fatemi, Ahmadreza; Razavi, Seyyed Mostafa; Asasi, Keramat; Goudarzi, Majid Torabi

    2015-03-01

    The present study aimed to compare the effect of different Artemisia annua extracts on sporulation rate of mixed oocysts of Eimeria acervulina, Eimeria necatrix, and Eimeria tenella. Three types of A. annua extracts including petroleum ether (PE), ethanol 96° (E), and water (W) extracts were prepared. Artemisinin, a sesquiterpene lactone endoperoxide derived from the A. annua analysis of each extract was done by high-performance liquid chromatography with ultraviolet detection (HPLC-UV). Fresh fecal samples containing three Eimeria species were floated and counted, and the oocysts were transferred into 50 tubes, each containing 10(5) oocysts per milliliter. Five tubes were control. Each of the other 45 tubes contained one of three doses (1 part per thousand (ppt), 2 ppt, and 5 ppt) and one of three extracts (PE, E, and W extracts) with five replications. The tubes were incubated for 48 h at 25-29 °C and aerated. Sporulation inhibition assay was used to evaluate the activity of extracts. The results showed that the E and PE extracts inhibit sporulation in 2 and 5 ppt concentrations, but the W extract stimulates it in all concentrations. The proportions of oocyst inhibition relative to control were 31 % (5 ppt) and 29 % (2 ppt) for PE and 34 % (5 ppt) and 46 % (2 ppt) for E extract. Furthermore, many oocysts in PE and E groups were wrinkled and contained abnormal sporocysts. The proportions of sporulation stimulation relative to control were 22 % (5 ppt), 24 % (2 ppt), and 27 % (1 ppt) in W extract. Our study is the first to demonstrate that all types of A. annua extracts do not necessarily have a similar activity, and the interaction of all contents and their relative concentrations is an important factor for sporulation stimulation or inhibition. It seems, some parts of unmetabolized excreted PE and E extracts could inhibit oocyst sporulation and eventually affect infection transmission.

  6. Global analysis of the sporulation pathway of Clostridium difficile.

    Science.gov (United States)

    Fimlaid, Kelly A; Bond, Jeffrey P; Schutz, Kristin C; Putnam, Emily E; Leung, Jacqueline M; Lawley, Trevor D; Shen, Aimee

    2013-01-01

    The Gram-positive, spore-forming pathogen Clostridium difficile is the leading definable cause of healthcare-associated diarrhea worldwide. C. difficile infections are difficult to treat because of their frequent recurrence, which can cause life-threatening complications such as pseudomembranous colitis. The spores of C. difficile are responsible for these high rates of recurrence, since they are the major transmissive form of the organism and resistant to antibiotics and many disinfectants. Despite the importance of spores to the pathogenesis of C. difficile, little is known about their composition or formation. Based on studies in Bacillus subtilis and other Clostridium spp., the sigma factors σ(F), σ(E), σ(G), and σ(K) are predicted to control the transcription of genes required for sporulation, although their specific functions vary depending on the organism. In order to determine the roles of σ(F), σ(E), σ(G), and σ(K) in regulating C. difficile sporulation, we generated loss-of-function mutations in genes encoding these sporulation sigma factors and performed RNA-Sequencing to identify specific sigma factor-dependent genes. This analysis identified 224 genes whose expression was collectively activated by sporulation sigma factors: 183 were σ(F)-dependent, 169 were σ(E)-dependent, 34 were σ(G)-dependent, and 31 were σ(K)-dependent. In contrast with B. subtilis, C. difficile σ(E) was dispensable for σ(G) activation, σ(G) was dispensable for σ(K) activation, and σ(F) was required for post-translationally activating σ(G). Collectively, these results provide the first genome-wide transcriptional analysis of genes induced by specific sporulation sigma factors in the Clostridia and highlight that diverse mechanisms regulate sporulation sigma factor activity in the Firmicutes.

  7. How Quorum Sensing Connects Sporulation to Necrotrophism in Bacillus thuringiensis.

    Science.gov (United States)

    Perchat, Stéphane; Talagas, Antoine; Poncet, Sandrine; Lazar, Noureddine; Li de la Sierra-Gallay, Inès; Gohar, Michel; Lereclus, Didier; Nessler, Sylvie

    2016-08-01

    Bacteria use quorum sensing to coordinate adaptation properties, cell fate or commitment to sporulation. The infectious cycle of Bacillus thuringiensis in the insect host is a powerful model to investigate the role of quorum sensing in natural conditions. It is tuned by communication systems regulators belonging to the RNPP family and directly regulated by re-internalized signaling peptides. One such RNPP regulator, NprR, acts in the presence of its cognate signaling peptide NprX as a transcription factor, regulating a set of genes involved in the survival of these bacteria in the insect cadaver. Here, we demonstrate that, in the absence of NprX and independently of its transcriptional activator function, NprR negatively controls sporulation. NprR inhibits expression of Spo0A-regulated genes by preventing the KinA-dependent phosphorylation of the phosphotransferase Spo0F, thus delaying initiation of the sporulation process. This NprR function displays striking similarities with the Rap proteins, which also belong to the RNPP family, but are devoid of DNA-binding domain and indirectly control gene expression via protein-protein interactions in Bacilli. Conservation of the Rap residues directly interacting with Spo0F further suggests a common inhibition of the sporulation phosphorelay. The crystal structure of apo NprR confirms that NprR displays a highly flexible Rap-like structure. We propose a molecular regulatory mechanism in which key residues of the bifunctional regulator NprR are directly and alternatively involved in its two functions. NprX binding switches NprR from a dimeric inhibitor of sporulation to a tetrameric transcriptional activator involved in the necrotrophic lifestyle of B. thuringiensis. NprR thus tightly coordinates sporulation and necrotrophism, ensuring survival and dissemination of the bacteria during host infection.

  8. Analysis of Bacillus subtilis sporulation with spore-converting bacteriophage PMB12.

    OpenAIRE

    Kinney, D M; Bramucci, M G

    1981-01-01

    Previous observations concerning the ability of the spore-converting bacteriophage PMB12 to cause sporulation in certain sporulation-deficient mutants of Bacillus subtilis 168 were extended to include a spoOK mutant and a mutant temperature sensitive for sporulation due to a ribosomal mutation. Mutants of PMB12 that were unable to induce sporulation in the spoOK mutant were isolated to determine whether PMB12-encoded products had to affect the sporulation-specific functions of both the transc...

  9. Factors Affecting Onset of Sporulation in Phytophthora ramorum

    Science.gov (United States)

    To elucidate the sporulation potential of the sudden oak death pathogen, Phytophthora ramorum, on rhododendron, we conducted a series of experiments looking at the relationship between moisture period, lesion size, and onset of sporangia production. Inoculations were performed using P. ramorum isol...

  10. Control of sporulation-specific cell division in Streptomyces coelicolor

    NARCIS (Netherlands)

    Noens, Elke

    2007-01-01

    During developmental cell division in sporulation-committed aerial hyphae of streptomycetes, up to a hundred septa are simultaneously produced, in close harmony with synchromous chromosome condensation and segregation. Several unique protein families are involved in the control of this process,

  11. Effect of different saccharides on growth, sporulation rate and d ...

    African Journals Online (AJOL)

    MFCS

    2012-05-17

    May 17, 2012 ... general, high sporulation rate was related with high growth rate and high viable cell count (>1.5 x 1012 cfu/ml). .... The sterile culture medium (180 ml) in a 1000 ml Erlenmeyer flask was ... The column temperature was set at 85°C. A series of ..... inactivation of certain sugar-metabolizing operons, such as lac ...

  12. Selectivity in protein degradation during sporulation of Bacillus subtilis

    International Nuclear Information System (INIS)

    Mitani, Takahiko; Kadota, Hajime

    1976-01-01

    The breakdown of cellular protein was investigated in Bacillus subtilis ATCC 6051 labeled with glycine-2- 3 H or L-phenylalanine-U- 14 C at the different stages of vegetative growth and sporulation. The growth of the culture was determined by measuring optical density at 660 nm. The heat-resistant spores were scored by plating after heating at 80 deg C for 10 minutes. A question whether the turnover of glycine-labeled protein is similar to that of phenylalanine-labeled protein was experimentally studied. The patterns obtained with the glycine-labeled protein were different from those of phenylalanine-labeled protein. This was not multiple turnover. The cellular protein which was labeled with glycine at an early stage of sporulation showed rapid degradation, but the degradation of the protein labeled with glycine at later stages did not occur at all. Another question whether the labeled glycine incorporated into cells at the different stages of growth and sporulation was present in the spore coat fraction of matured spores was studied. Experiment demonstrated that the glycine incorporated into cells at the late sporulation stage was mainly utilized for the biosynthesis of the spore coat protein. These data suggest that the spore coat protein which contains relatively large amount of glycine is rarely subject to further degradation. (Iwakiri, K.)

  13. Effect of health-promoting posters placed on the platforms of two train stations in Copenhagen, Denmark, on the choice between taking the stairs or the escalators

    DEFF Research Database (Denmark)

    Iversen, Mette Kathrine; Händel, M N; Nydal Jensen, Eva

    2007-01-01

    OBJECTIVE: The purpose of this study was to determine whether posters placed on the platforms of two train stations in Copenhagen, promoting use of the stairs, would encourage people to use the stairs rather than the adjacent escalator. An additional purpose was to see if the effect of the interv......OBJECTIVE: The purpose of this study was to determine whether posters placed on the platforms of two train stations in Copenhagen, promoting use of the stairs, would encourage people to use the stairs rather than the adjacent escalator. An additional purpose was to see if the effect...... of the intervention was maintained for a week after the poster was removed. MEASUREMENTS: The number of people using stairs and escalators at Copenhagen Central Station and Østerport Train Station in Copenhagen was recorded before and during posters promoting stair use were placed on the platforms, and a week after...... the posters were removed. Two years after the posters were removed, data were collected for 1 week at Østerport Train Station (long-term post-intervention). RESULTS: At Copenhagen Central Station, the overall stair use increased from 12% before the intervention to 16% (P

  14. SUB1 Plays a Negative Role during Starvation Induced Sporulation Program in Saccharomyces cerevisiae.

    Science.gov (United States)

    Gupta, Ritu; Sadhale, Parag P; Vijayraghavan, Usha

    2015-01-01

    Saccharomyces cerevisiae Sub1 is involved in several cellular processes such as, transcription initiation, elongation, mRNA processing and DNA repair. It has also been reported to provide cellular resistance during conditions of oxidative DNA damage and osmotic stress. Here, we report a novel role of SUB1 during starvation stress-induced sporulation, which leads to meiosis and spore formation in diploid yeast cells. Deletion of SUB1 gene significantly increased sporulation efficiency as compared to the wild-type cells in S288c genetic background. Whereas, the sporulation functions of the sub1(Y66A) missense mutant were similar to Sub1. SUB1 transcript and protein levels are downregulated during sporulation, in highly synchronized and sporulation proficient wild-type SK1 cells. The changes in Sub1 levels during sporulation cascade correlate with the induction of middle sporulation gene expression. Deletion of SUB1 increased middle sporulation gene transcript levels with no effect on their induction kinetics. In wild-type cells, Sub1 associates with chromatin at these loci in a temporal pattern that correlates with their enhanced gene expression seen in sub1Δ cells. We show that SUB1 genetically interacts with HOS2, which led us to speculate that Sub1 might function with Set3 repressor complex during sporulation. Positive Cofactor 4, human homolog of Sub1, complemented the sub1Δ sporulation phenotype, suggesting conservation of function. Taken together, our results suggest that SUB1 acts as a negative regulator of sporulation.

  15. NS [Nuclear Safety] update. Current safety and security activities and developments taking place in the Department of Nuclear Safety and Security, Issue no. 6, March 2008

    International Nuclear Information System (INIS)

    2008-03-01

    The current issue presents information about the following activities: 1) International Conference on Illicit Nuclear Trafficking which took place in November 2007 in Edinburgh. The principal aim of the conference was to examine the threat and context of illicit nuclear trafficking of radioactive material, specifically, what is being done to combat such trafficking and where more needs to be done. The conference was also to consider how the obligations and commitments of the legally binding and non-binding international instruments could be and are being implemented by various States. 2) INSAG Message on Nuclear Safety Infrastructure in which the INSAG Chairman Richard Meserve addressed nuclear safety in the current context and various issues that warrant special attention. 3) approved for publication the Safety Requirements publication on Safety of Nuclear Fuel Cycle Facilities. 4) The Asian Nuclear Safety Network (ANSN)

  16. NS [Nuclear Safety] update. Current safety and security activities and developments taking place in the Department of Nuclear Safety and Security, Issue no. 8, September 2008

    International Nuclear Information System (INIS)

    2008-09-01

    The current issue presents information about the following activities: 1) International Workshops on Denial of Shipments raise awareness of suppliers, recipients, regulators, carriers/consignors and international organizations of the problems relating to denials of radioactive shipments to determine effective measures to prevent or reduce the instances of shipment denials and delays. 2) Communication and knowledge Management in the Department of Nuclear Safety and Security (NS). 3) Nuclear Security at the Beijing Olympics - an excellent example of the IAEA's work in protecting large scale public events. 4) The Incident and Emergency Centre's Participation in the ConvEx 3 Exercise, 9-10 July 2008, which took place at the Laguna Verde nuclear power plant in Mexico. During the 43 hour long exercise, the Incident and Emergency Centre (IEC) was fully activated. Staff members participating in the exercise represented different departments within the IAEA and the diversity of their knowledge and experience ensured an effective response

  17. NS [Nuclear Safety] update. Current safety and security activities and developments taking place in the Department of Nuclear Safety and Security. Issue no. 2, January 2007

    International Nuclear Information System (INIS)

    2006-08-01

    This newsletter reports on the training of cardiologists in radiation protection, IAEA's safety review services and the operational safety assessment review team (OSART), the international conference on management of spent fuel and the recent INSAG (International Nuclear Safety Group) publications. The IAEA has begun a major international initiative to train interventional cardiologists in radiation protection. Starting with the first course in May 2004, so far 6 regional and 3 national training courses have been conducted with the participation of over 400 health professionals putting the IAEA in a leading role in this area. A programme of two days' training has been developed, covering possible and observed radiation effects among patients and staff, international standards, dose management techniques, examples of good and bad practice and examples indicating prevention of possible injuries as a result of good practice in radiation protection. The training material is freely available on CD and will be placed on the Radiological Protection of Patients website at http://rpop.iaea.org/

  18. Genome-Wide Transcriptional Profiling of Clostridium perfringens SM101 during Sporulation Extends the Core of Putative Sporulation Genes and Genes Determining Spore Properties and Germination Characteristics

    NARCIS (Netherlands)

    Xiao, Y.; Hijum, S.A.F.T. van; Abee, T.; Wells-Bennik, M.H.

    2015-01-01

    The formation of bacterial spores is a highly regulated process and the ultimate properties of the spores are determined during sporulation and subsequent maturation. A wide variety of genes that are expressed during sporulation determine spore properties such as resistance to heat and other adverse

  19. Genome-wide transcriptional profiling of Clostridium perfringens SM101 during sporulation extends the core of putative sporulation genes and genes determining spore properties and germination characteristics

    NARCIS (Netherlands)

    Xiao, Y.; Hijum, van S.A.F.T.; Abee, T.; Wells-Bennik, M.H.J.

    2015-01-01

    The formation of bacterial spores is a highly regulated process and the ultimate properties of the spores are determined during sporulation and subsequent maturation. A wide variety of genes that are expressed during sporulation determine spore properties such as resistance to heat and other adverse

  20. Place-focused physical activity research, human agency, and social justice in public health: taking agency seriously in studies of the built environment.

    Science.gov (United States)

    Blacksher, Erika; Lovasi, Gina S

    2012-03-01

    Built environment characteristics have been linked to health outcomes and health disparities. However, the effects of an environment on behavior may depend on human perception, interpretation, motivation, and other forms of human agency. We draw on epidemiological and ethical concepts to articulate a critique of research on the built environment and physical activity. We identify problematic assumptions and enumerate both scientific and ethical reasons to incorporate subjective perspectives and public engagement strategies into built environment research and interventions. We maintain that taking agency seriously is essential to the pursuit of health equity and the broader demands of social justice in public health, an important consideration as studies of the built environment and physical activity increasingly focus on socially disadvantaged communities. Attention to how people understand their environment and navigate competing demands can improve the scientific value of ongoing efforts to promote active living and health, while also better fulfilling our ethical obligations to the individuals and communities whose health we strive to protect. Copyright © 2011 Elsevier Ltd. All rights reserved.

  1. A novel non prophage(-like) gene-intervening element within gerE that is reconstituted during sporulation in Bacillus cereus ATCC10987.

    Science.gov (United States)

    Abe, Kimihiro; Shimizu, Shin-Ya; Tsuda, Shuhei; Sato, Tsutomu

    2017-09-12

    Gene rearrangement is a widely-shared phenomenon in spore forming bacteria, in which prophage(-like) elements interrupting sporulation-specific genes are excised from the host genome to reconstitute the intact gene. Here, we report a novel class of gene-intervening elements, named gin, inserted in the 225 bp gerE-coding region of the B. cereus ATCC10987 genome, which generates a sporulation-specific rearrangement. gin has no phage-related genes and possesses three site-specific recombinase genes; girA, girB, and girC. We demonstrated that the gerE rearrangement occurs at the middle stage of sporulation, in which site-specific DNA recombination took place within the 9 bp consensus sequence flanking the disrupted gerE segments. Deletion analysis of gin uncovered that GirC and an additional factor, GirX, are responsible for gerE reconstitution. Involvement of GirC and GirX in DNA recombination was confirmed by an in vitro recombination assay. These results broaden the definition of the sporulation-specific gene rearrangement phenomenon: gene-intervening elements are not limited to phage DNA but may include non-viral genetic elements that carry a developmentally-regulated site-specific recombination system.

  2. Leaf processing behaviour in Atta leafcutter ants: 90% of leaf cutting takes place inside the nest, and ants select pieces that require less cutting.

    Science.gov (United States)

    Garrett, Ryan W; Carlson, Katherine A; Goggans, Matthew Scott; Nesson, Michael H; Shepard, Christopher A; Schofield, Robert M S

    2016-01-01

    Leafcutter ants cut trimmings from plants, carry them to their underground nests and cut them into smaller pieces before inoculating them with a fungus that serves as a primary food source for the colony. Cutting is energetically costly, so the amount of cutting is important in understanding foraging energetics. Estimates of the cutting density, metres of cutting per square metre of leaf, were made from samples of transported leaf cuttings and of fungal substrate from field colonies of Atta cephalotes and Atta colombica. To investigate cutting inside the nest, we made leaf-processing observations of our laboratory colony, A. cephalotes. We did not observe the commonly reported reduction of the leaf fragments into a pulp, which would greatly increase the energy cost of processing. Video clips of processing behaviours, including behaviours that have not previously been described, are linked. An estimated 2.9 (±0.3) km of cutting with mandibles was required to reduce a square metre of leaf to fungal substrate. Only about 12% (±1%) of this cutting took place outside of the nest. The cutting density and energy cost is lower for leaf material with higher ratios of perimeter to area, so we tested for, and found that the laboratory ants had a preference for leaves that were pre-cut into smaller pieces. Estimates suggest that the energy required to transport and cut up the leaf material is comparable to the metabolic energy available from the fungus grown on the leaves, and so conservation of energy is likely to be a particularly strong selective pressure for leafcutter ants.

  3. Hypoxia and inactivity related physiological changes precede or take place in absence of significant rearrangements in bacterial community structure: The PlanHab randomized trial pilot study.

    Directory of Open Access Journals (Sweden)

    Robert Šket

    Full Text Available We explored the assembly of intestinal microbiota in healthy male participants during the randomized crossover design of run-in (5 day and experimental phases (21-day normoxic bed rest (NBR, hypoxic bed rest (HBR and hypoxic ambulation (HAmb in a strictly controlled laboratory environment, with balanced fluid and dietary intakes, controlled circadian rhythm, microbial ambiental burden and 24/7 medical surveillance. The fraction of inspired O2 (FiO2 and partial pressure of inspired O2 (PiO2 were 0.209 and 133.1 ± 0.3 mmHg for NBR and 0.141 ± 0.004 and 90.0 ± 0.4 mmHg for both hypoxic variants (HBR and HAmb; ~4000 m simulated altitude, respectively. A number of parameters linked to intestinal environment such as defecation frequency, intestinal electrical conductivity (IEC, sterol and polyphenol content and diversity, indole, aromaticity and spectral characteristics of dissolved organic matter (DOM were measured (64 variables. The structure and diversity of bacterial microbial community was assessed using 16S rRNA amplicon sequencing. Inactivity negatively affected frequency of defecation and in combination with hypoxia increased IEC (p < 0.05. In contrast, sterol and polyphenol diversity and content, various characteristics of DOM and aromatic compounds, the structure and diversity of bacterial microbial community were not significantly affected over time. A new in-house PlanHab database was established to integrate all measured variables on host physiology, diet, experiment, immune and metabolic markers (n = 231. The observed progressive decrease in defecation frequency and concomitant increase in IEC suggested that the transition from healthy physiological state towards the developed symptoms of low magnitude obesity-related syndromes was dose dependent on the extent of time spent in inactivity and preceded or took place in absence of significant rearrangements in bacterial microbial community. Species B. thetaiotamicron, B. fragilis, B

  4. Streptomyces sporulation - Genes and regulators involved in bacterial cell differentiation

    OpenAIRE

    Larsson, Jessica

    2010-01-01

    Streptomycetes are Gram-positive bacteria with a complex developmental life cycle. They form spores on specialized cells called aerial hyphae, and this sporulation involves alterations in growth, morphogenesis and cell cycle processes like cell division and chromosome segregation. Understanding the developmental mechanisms that streptomycetes have evolved for regulating for example cell division is of general interest in bacterial cell biology. It can also be valuable in the design of new dru...

  5. Effects of whey on the colonization and sporulation of arbuscular ...

    African Journals Online (AJOL)

    The aim of this study is to research the effect of 2 different doses of whey [50 ml kg-1(W50) and 100 ml kg-1(W100)], an important organic waste, on colonization and sporulation of arbuscular mycorhizal fungus (AMF) Glomus intraradices'(G.i.) inoculated to lentil plant and the effects of changing P ratio in the soil and plant as ...

  6. Designated Places

    Data.gov (United States)

    California Natural Resource Agency — Census 2000 Place Names provides a seamless statewide GIS layer of places, including census designated places (CDP), consolidated cities, and incorporated places,...

  7. Nitrate salts suppress sporulation and production of enterotoxin in Clostridium perfringens strain NCTC8239.

    Science.gov (United States)

    Yasugi, Mayo; Otsuka, Keisuke; Miyake, Masami

    2016-10-01

    Clostridium perfringens type A is a common source of food-borne illness in humans. Ingested vegetative cells sporulate in the small intestinal tract and in the process produce C. perfringens enterotoxin (CPE). Although sporulation plays a critical role in the pathogenesis of food-borne illness, the molecules triggering/inhibiting sporulation are still largely unknown. It has previously been reported by our group that sporulation is induced in C. perfringens strain NCTC8239 co-cultured with Caco-2 cells in Dulbecco's Modified Eagle Medium (DMEM). In contrast, an equivalent amount of spores was not observed when bacteria were co-cultured in Roswell Park Memorial Institute-1640 medium (RPMI). In the present study it was found that, when these two media are mixed, RPMI inhibits sporulation and CPE production induced in DMEM. When a component of RPMI was added to DMEM, it was found that calcium nitrate (Ca[NO 3 ] 2 ) significantly inhibits sporulation and CPE production. The number of spores increased when Ca(NO 3 ) 2 -deficient RPMI was used. The other nitrate salts significantly suppressed sporulation, whereas the calcium salts used did not. qPCR revealed that nitrate salts increased expression of bacterial nitrate/nitrite reductase. Furthermore, it was found that nitrite and nitric oxide suppress sporulation. In the sporulation stages, Ca(NO 3 ) 2 down-regulated the genes controlled by Spo0A, a master regulator of sporulation, but not spo0A itself. Collectively, these results indicate that nitrate salts suppress sporulation and CPE production by down-regulating Spo0A-regulated genes in C. perfringens strain NCTC8239. Nitrate reduction may be associated with inhibition of sporulation. © 2016 The Societies and John Wiley & Sons Australia, Ltd.

  8. Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae.

    OpenAIRE

    Enyenihi, Akon H; Saunders, William S

    2003-01-01

    We have used a single-gene deletion mutant bank to identify the genes required for meiosis and sporulation among 4323 nonessential Saccharomyces cerevisiae annotated open reading frames (ORFs). Three hundred thirty-four sporulation-essential genes were identified, including 78 novel ORFs and 115 known genes without previously described sporulation defects in the comprehensive Saccharomyces Genome (SGD) or Yeast Proteome (YPD) phenotype databases. We have further divided the uncharacterized sp...

  9. Pre-sporulation stages of Streptomyces differentiation: state-of-the-art and future perspectives

    Science.gov (United States)

    Yagüe, Paula; López-García, Maria T.; Rioseras, Beatriz; Sánchez, Jesús; Manteca, Ángel

    2013-01-01

    Streptomycetes comprise very important industrial bacteria, producing two-thirds of all clinically relevant secondary metabolites. They are mycelial microorganisms with complex developmental cycles that include programmed cell death (PCD) and sporulation. Industrial fermentations are usually performed in liquid cultures (large bioreactors), conditions in which Streptomyces strains generally do not sporulate, and it was traditionally assumed that there was no differentiation. In this work, we review the current knowledge on Streptomyces pre-sporulation stages of Streptomyces differentiation. PMID:23496097

  10. Temporal Expression of a Master Regulator Drives Synchronous Sporulation in Budding Yeast

    Directory of Open Access Journals (Sweden)

    Minghao Chia

    2016-11-01

    Full Text Available Yeast cells enter and undergo gametogenesis relatively asynchronously, making it technically challenging to perform stage-specific genomic and biochemical analyses. Cell-to-cell variation in the expression of the master regulator of entry into sporulation, IME1, has been implicated to be the underlying cause of asynchronous sporulation. Here, we find that timing of IME1 expression is of critical importance for inducing cells to undergo sporulation synchronously. When we force expression of IME1 from an inducible promoter in cells incubated in sporulation medium for 2 hr, the vast majority of cells exhibit synchrony during premeiotic DNA replication and meiotic divisions. Inducing IME1 expression too early or too late affects the synchrony of sporulation. Surprisingly, our approach for synchronous sporulation does not require growth in acetate-containing medium, but can be achieved in cells grown in rich medium until saturation. Our system requires solely IME1, because the expression of the N6-methyladenosine methyltransferase IME4, another key regulator of early sporulation, is controlled by IME1 itself. The approach described here can be combined easily with other stage-specific synchronization methods, and thereby applied to study specific stages of sporulation, or the complete sporulation program.

  11. Substitution of the radiation requirement for sporulation by host tissue in Dendrophoma obscurans

    International Nuclear Information System (INIS)

    Binder, F.L.; Lilly, V.G.

    1975-01-01

    Cultures of Dendrophoma obscurans growing on a 10--2 glucose-casein hydrolysate medium required radiation for sporulation. When leaf discs from host tissue or certain plants, particularly members of the Rosaceae, were added to the basal medium, sporulation occurred abundantly in darkness on the leaf surface. The ability to replace the radiation requirement for sporulation did not occur with all plant tissue tested. The active substance in powdered host tissue could be extracted with certain organic solvents but not with water. This crude extract could be concentrated, autoclaved, and when added to the basal medium supported sporulation in darkness

  12. Temporal Expression of a Master Regulator Drives Synchronous Sporulation in Budding Yeast.

    Science.gov (United States)

    Chia, Minghao; van Werven, Folkert J

    2016-09-07

    Yeast cells enter and undergo gametogenesis relatively asynchronously, making it technically challenging to perform stage-specific genomic and biochemical analyses. Cell-to-cell variation in the expression of the master regulator of entry into sporulation IME1, has been implicated to be the underlying cause of asynchronous sporulation. Here we find that timing of IME1 expression is of critical importance for inducing cells to undergo sporulation synchronously. When we force expression of IME1 from an inducible promoter in cells incubated in sporulation medium for two hours, the vast majority of cells exhibit synchrony during pre-meiotic DNA replication and meiotic divisions. Inducing IME1 expression too early or too late affects the synchrony of sporulation. Surprisingly, our approach for synchronous sporulation does not require growth in acetate containing medium, but can be achieved in cells grown in rich medium until saturation. Our system solely requires IME1 because the expression of the N6-methyladenosine methyltransferase IME4, another key regulator of early sporulation, is controlled by IME1 itself. The approach described here can be easily combined with other stage specific synchronization methods, and thereby applied to study specific stages of sporulation or the complete sporulation program. Copyright © 2016 Author et al.

  13. Transcriptional Profile during Deoxycholate-Induced Sporulation in a Clostridium perfringens Isolate Causing Foodborne Illness.

    Science.gov (United States)

    Yasugi, Mayo; Okuzaki, Daisuke; Kuwana, Ritsuko; Takamatsu, Hiromu; Fujita, Masaya; Sarker, Mahfuzur R; Miyake, Masami

    2016-05-15

    Clostridium perfringens type A is a common source of foodborne illness (FBI) in humans. Vegetative cells sporulate in the small intestinal tract and produce the major pathogenic factor C. perfringens enterotoxin. Although sporulation plays a critical role in the pathogenesis of FBI, the mechanisms inducing sporulation remain unclear. Bile salts were shown previously to induce sporulation, and we confirmed deoxycholate (DCA)-induced sporulation in C. perfringens strain NCTC8239 cocultured with human intestinal epithelial Caco-2 cells. In the present study, we performed transcriptome analyses of strain NCTC8239 in order to elucidate the mechanism underlying DCA-induced sporulation. Of the 2,761 genes analyzed, 333 were up- or downregulated during DCA-induced sporulation and included genes for cell division, nutrient metabolism, signal transduction, and defense mechanisms. In contrast, the virulence-associated transcriptional regulators (the VirR/VirS system, the agr system, codY, and abrB) were not activated by DCA. DCA markedly increased the expression of signaling molecules controlled by Spo0A, the master regulator of the sporulation process, whereas the expression of spo0A itself was not altered in the presence or absence of DCA. The phosphorylation of Spo0A was enhanced in the presence of DCA. Collectively, these results demonstrated that DCA induced sporulation, at least partially, by facilitating the phosphorylation of Spo0A and activating Spo0A-regulated genes in strain NCTC8239 while altering the expression of various genes. Disease caused by Clostridium perfringens type A consistently ranks among the most common bacterial foodborne illnesses in humans in developed countries. The sporulation of C. perfringens in the small intestinal tract is a key event for its pathogenesis, but the factors and underlying mechanisms by which C. perfringens sporulates in vivo currently remain unclear. Bile salts, major components of bile, which is secreted from the liver for

  14. Selective inhibition of Bacillus subtilis sporulation by acridine orange and promethazine.

    Science.gov (United States)

    Burke, W F; Spizizen, J

    1977-03-01

    Two structurally similar compounds were found to inhibit sporulation in Bacillus subtilis 168. A dye, acridine orange, and an antischizophrenic drug, promethazine, blocked spore formation at concentrations subinhibitory to vegetative growth, while allowing synthesis of serine protease, antibiotic, and certain catabolite-repressed enzymes. The sporulation process was sensitive to promethazine through T2, whereas acridine orange was inhibitory until T4. The drug-treated cells were able to support the replication of phages phie and phi29, although the lytic cycles were altered slightly. The selective inhibition of sporulation by these compounds may be related to the affinity of some sporulation-specific genes to intercalating compounds.

  15. Major growth in coke production takes place

    International Nuclear Information System (INIS)

    Swain, E.J.

    1991-01-01

    U.S. petroleum coke production has increased 64% during the 10-year period from 1980 to 1990. This dramatic rise makes it timely to discuss the history and future of U.S. coking capacity, production, and processing. The article covers the properties and uses of the various grades of petroleum coke, as well as pricing and market trends

  16. Proteomic signatures differentiating Bacillus anthracis Sterne sporulation on soil relative to laboratory media.

    Science.gov (United States)

    Wunschel, D S; Hutchison, J R; Deatherage Kaiser, B L; Merkley, E D; Hess, B M; Lin, A; Warner, M G

    2017-12-18

    The process of sporulation is vital for the stability and infectious cycle of Bacillus anthracis. The spore is the infectious form of the organism and therefore relevant to biodefense. While the morphological and molecular events occurring during sporulation have been well studied, the influence of growth medium and temperature on the proteins expressed in sporulated cultures is not well understood. Understanding the features of B. anthracis sporulation specific to natural vs. laboratory production will address an important question in microbial forensics. In an effort to bridge this knowledge gap, a system for sporulation on two types of agar-immobilized soils was used for comparison to cultures sporulated on two common types of solid laboratory media, and one liquid sporulation medium. The total number of proteins identified as well as their identity differed between samples generated in each medium and growth temperature, demonstrating that sporulation environment significantly impacts the protein content of the spore. In addition, a subset of proteins common in all of the soil-cultivated samples was distinct from the expression profiles in laboratory medium (and vice versa). These differences included proteins involved in thiamine and phosphate metabolism in the sporulated cultures produced on soils with a notable increase in expression of ATP binding cassette (ABC) transporters annotated to be for phosphate and antimicrobial peptides. A distinct set of ABC transporters for amino acids, sugars and oligopeptides were found in cultures produced on laboratory media as well as increases in carbon and amino acid metabolism-related proteins. These protein expression changes indicate that the sporulation environment impacts the protein profiles in specific ways that are reflected in the metabolic and membrane transporter proteins present in sporulated cultures.

  17. Repeated triggering of sporulation in Bacillus subtilis selects against a protein that affects the timing of cell division

    NARCIS (Netherlands)

    Siebring, Jeroen; Elema, Matthijs J. H.; Vega, Fatima Drubi; Kovacs, Akos T.; Haccou, Patsy; Kuipers, Oscar P.

    Bacillus subtilis sporulation is a last-resort phenotypical adaptation in response to starvation. The regulatory network underlying this developmental pathway has been studied extensively. However, how sporulation initiation is concerted in relation to the environmental nutrient availability is

  18. The effect of salinity on the growth, sporulation and infection of Phytophthora ramorum

    Science.gov (United States)

    Phytophthora ramorum, a threat to Eastern U.S. forests, has been found in waterways outside the boundaries of infested ornamental nurseries. Very little is known about what factors are conducive to its survival and sporulation in water. This study examined the effect of salt on growth, sporulation,...

  19. Genome-wide transcriptional profiling of Clostridium perfringens SM101 during sporulation

    NARCIS (Netherlands)

    Xiao, Yinghua; Hijum, van Sacha A.; Abee, Tjakko; Wells-Bennik, Marjon H.

    2014-01-01

    In this study we focus on the identification of new genes tentatively involved in sporulation and those that influence properties of spores and their ability to germinate. To this end, the sporulation stages of C. perfringens enterotoxic strain SM101 were characterized based on morphological

  20. Effect of plant sterols and tannins on Phytophthora ramorum growth and sporulation

    Science.gov (United States)

    The acquisition of plant sterols, mediated via elicitins, is required for growth and sporulation of Phytophthora spp. In this paper, we looked at the interaction between elicitins, sterols, and tannins. When ground leaf tissue was added to growth media, P. ramorum growth and sporulation was greates...

  1. Phosphatases modulate the bistable sporulation gene expression pattern in Bacillus subtilis

    NARCIS (Netherlands)

    Veening, JW; Hamoen, LW; Kuipers, OP

    Spore formation in the Gram- positive bacterium Bacillus subtilis is a last resort adaptive response to starvation. To initiate sporulation, the key regulator in this process, Spo0A, needs to be activated by the so-called phosphorelay. Within a sporulating culture of B. subtilis, some cells initiate

  2. A mechanism for cell cycle regulation of sporulation initiation in Bacillus subtilis

    NARCIS (Netherlands)

    Veening, Jan-Willem; Murray, Heath; Errington, Jeff

    2009-01-01

    Coordination of DNA replication with cellular development is a crucial problem in most living organisms. Bacillus subtilis cells switch from vegetative growth to sporulation when starved. Sporulation normally occurs in cells that have stopped replicating DNA and have two completed chromosomes: one

  3. Sporulation of Bremia lactucae affected by temperature, relative humidity, and wind in controlled conditions

    NARCIS (Netherlands)

    Su, H.; Bruggen, van A.H.C.; Subbarao, K.V.; Scherm, H.

    2004-01-01

    The effects of temperature (5 to 25degreesC), relative humidity (81 to 100%), wind speed (0 to 1.0 in s(-1)), and their interactions on sporulation of Bremia lactucae on lettuce cotyledons were investigated in controlled conditions. Sporulation was affected significantly (P <0.0001) by

  4. Sporulation and survival of Toxoplasma gondii oocysts in different types of commercial cat litters

    Science.gov (United States)

    Toxoplasma gondii oocysts are environmentally resistant and can survive outdoors for months in the dry and cold climates. In the present study, sporulation and survival of T. gondii oocysts was studied in different types of cat litters commercially available in the US. Oocysts sporulated within 2-...

  5. Sporulation dynamics and spore heat resistance in wet and dry biofilms of Bacillus cereus

    NARCIS (Netherlands)

    Hayrapetyan, Hasmik; Abee, Tjakko; Nierop Groot, Masja

    2016-01-01

    Environmental conditions and growth history can affect the sporulation process as well as subsequent properties of formed spores. The sporulation dynamics was studied in wet and air-dried biofilms formed on stainless steel (SS) and polystyrene (PS) for Bacillus cereus ATCC 10987 and the

  6. Genome-Wide Transcriptional Profiling of Clostridium perfringens SM101 during Sporulation Extends the Core of Putative Sporulation Genes and Genes Determining Spore Properties and Germination Characteristics.

    Science.gov (United States)

    Xiao, Yinghua; van Hijum, Sacha A F T; Abee, Tjakko; Wells-Bennik, Marjon H J

    2015-01-01

    The formation of bacterial spores is a highly regulated process and the ultimate properties of the spores are determined during sporulation and subsequent maturation. A wide variety of genes that are expressed during sporulation determine spore properties such as resistance to heat and other adverse environmental conditions, dormancy and germination responses. In this study we characterized the sporulation phases of C. perfringens enterotoxic strain SM101 based on morphological characteristics, biomass accumulation (OD600), the total viable counts of cells plus spores, the viable count of heat resistant spores alone, the pH of the supernatant, enterotoxin production and dipicolinic acid accumulation. Subsequently, whole-genome expression profiling during key phases of the sporulation process was performed using DNA microarrays, and genes were clustered based on their time-course expression profiles during sporulation. The majority of previously characterized C. perfringens germination genes showed upregulated expression profiles in time during sporulation and belonged to two main clusters of genes. These clusters with up-regulated genes contained a large number of C. perfringens genes which are homologs of Bacillus genes with roles in sporulation and germination; this study therefore suggests that those homologs are functional in C. perfringens. A comprehensive homology search revealed that approximately half of the upregulated genes in the two clusters are conserved within a broad range of sporeforming Firmicutes. Another 30% of upregulated genes in the two clusters were found only in Clostridium species, while the remaining 20% appeared to be specific for C. perfringens. These newly identified genes may add to the repertoire of genes with roles in sporulation and determining spore properties including germination behavior. Their exact roles remain to be elucidated in future studies.

  7. [Regulation of sporulation by two-component system YvcPQ in Bacillus thuringiensis].

    Science.gov (United States)

    Fan, Qingyun; Zhang, Shumeng; Gong, Yujing; He, Jin

    2017-01-04

    To study the regulation of sporulation controlled by two-component system (TCS) YvcPQ. β-galactosidase experiment was used to verify the regulation of YvcP on kapD expression; bacterial one-hybrid assay, EMSA and RT-qPCR were applied to study the regulation of AbrB on yvcPQ expression; markerless gene deletion coupled with spore count was used to reveal the influence of yvcPQ and kapD expressions on sporulation. transcriptional regulator AbrB up-regulated the expression of yvcPQ; YvcP promoted the expression of kapD to inhibit sporulation. AbrB up-regulated the transcription of yvcPQ operon, then the increased YvcP strengthened the transcriptional acitivation of sporulation inhibitor gene kapD, and subsequently inhibited sporulation.

  8. Initiation of sporulation in Clostridium difficile: a twist on the classic model.

    Science.gov (United States)

    Edwards, Adrianne N; McBride, Shonna M

    2014-09-01

    The formation of dormant endospores is a complex morphological process that permits long-term survival in inhospitable environments for many Gram-positive bacteria. Sporulation for the anaerobic gastrointestinal pathogen Clostridium difficile is necessary for survival outside of the gastrointestinal tract of its host. While the developmental stages of spore formation are largely conserved among endospore-forming bacteria, the genus Clostridium appears to be missing a number of conserved regulators required for efficient sporulation in other spore-forming bacteria. Several recent studies have discovered novel mechanisms and distinct regulatory pathways that control the initiation of sporulation and early-sporulation-specific gene expression. These differences in regulating the decision to undergo sporulation reflects the unique ecological niche and environmental conditions that C. difficile inhabits and encounters within the mammalian host. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

  9. Global iTRAQ-based proteomic profiling of Toxoplasma gondii oocysts during sporulation.

    Science.gov (United States)

    Zhou, Chun-Xue; Zhu, Xing-Quan; Elsheikha, Hany M; He, Shuai; Li, Qian; Zhou, Dong-Hui; Suo, Xun

    2016-10-04

    Toxoplasma gondii is a medically and economically important protozoan parasite. However, the molecular mechanisms of its sporulation remain largely unknown. Here, we applied iTRAQ coupled with 2D LC-MS/MS proteomic analysis to investigate the proteomic expression profile of T. gondii oocysts during sporulation. Of the 2095 non-redundant proteins identified, 587 were identified as differentially expressed proteins (DEPs). Based on Gene Ontology enrichment and KEGG pathway analyses the majority of these DEPs were found related to the metabolism of amino acids, carbon and energy. Protein interaction network analysis generated by STRING identified ATP-citrate lyase (ACL), GMP synthase, IMP dehydrogenase (IMPDH), poly (ADP-ribose) glycohydrolase (PARG), and bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS) as the top five hubs. We also identified 25 parasite virulence factors that were expressed at relatively high levels in sporulated oocysts compared to non-sporulated oocysts, which might contribute to the infectivity of mature oocysts. Considering the importance of oocysts in the dissemination of toxoplasmosis these findings may help in the search of protein targets with a key role in infectiousness and ecological success of oocysts, creating new opportunities for the development of better means for disease prevention. The development of new preventative interventions against T. gondii infection relies on an improved understanding of the proteome and chemical pathways of this parasite. To identify proteins required for the development of environmentally resistant and infective T. gondii oocysts, we compared the proteome of non-sporulated (immature) oocysts with the proteome of sporulated (mature, infective) oocysts. iTRAQ 2D-LC-MS/MS analysis revealed proteomic changes that distinguish non-sporulated from sporulated oocysts. Many of the differentially expressed proteins were involved in metabolic pathways and 25 virulence factors were identified

  10. Involvement of Clostridium botulinum ATCC 3502 sigma factor K in early-stage sporulation.

    Science.gov (United States)

    Kirk, David G; Dahlsten, Elias; Zhang, Zhen; Korkeala, Hannu; Lindström, Miia

    2012-07-01

    A key survival mechanism of Clostridium botulinum, the notorious neurotoxic food pathogen, is the ability to form heat-resistant spores. While the genetic mechanisms of sporulation are well understood in the model organism Bacillus subtilis, nothing is known about these mechanisms in C. botulinum. Using the ClosTron gene-knockout tool, sigK, encoding late-stage (stage IV) sporulation sigma factor K in B. subtilis, was disrupted in C. botulinum ATCC 3502 to produce two different mutants with distinct insertion sites and orientations. Both mutants were unable to form spores, and their elongated cell morphology suggested that the sporulation pathway was blocked at an early stage. In contrast, sigK-complemented mutants sporulated successfully. Quantitative real-time PCR analysis of sigK in the parent strain revealed expression at the late log growth phase in the parent strain. Analysis of spo0A, encoding the sporulation master switch, in the sigK mutant and the parent showed significantly reduced relative levels of spo0A expression in the sigK mutant compared to the parent strain. Similarly, sigF showed significantly lower relative transcription levels in the sigK mutant than the parent strain, suggesting that the sporulation pathway was blocked in the sigK mutant at an early stage. We conclude that σ(K) is essential for early-stage sporulation in C. botulinum ATCC 3502, rather than being involved in late-stage sporulation, as reported for the sporulation model organism B. subtilis. Understanding the sporulation mechanism of C. botulinum provides keys to control the public health risks that the spores of this dangerous pathogen cause through foods.

  11. 6S-1 RNA function leads to a delay in sporulation in Bacillus subtilis.

    Science.gov (United States)

    Cavanagh, Amy T; Wassarman, Karen M

    2013-05-01

    We have discovered that 6S-1 RNA (encoded by bsrA) is important for appropriate timing of sporulation in Bacillus subtilis in that cells lacking 6S-1 RNA sporulate earlier than wild-type cells. The time to generate a mature spore once the decision to sporulate has been made is unaffected by 6S-1 RNA, and, therefore, we propose that it is the timing of onset of sporulation that is altered. Interestingly, the presence of cells lacking 6S-1 RNA in coculture leads to all cell types exhibiting an early-sporulation phenotype. We propose that cells lacking 6S-1 RNA modify their environment in a manner that promotes early sporulation. In support of this model, resuspension of wild-type cells in conditioned medium from ΔbsrA cultures also resulted in early sporulation. Use of Escherichia coli growth as a reporter of the nutritional status of conditioned media suggested that B. subtilis cells lacking 6S-1 RNA reduce the nutrient content of their environment earlier than wild-type cells. Several pathways known to impact the timing of sporulation, such as the skf- and sdp-dependent cannibalism pathways, were eliminated as potential targets of 6S-1 RNA-mediated changes, suggesting that 6S-1 RNA activity defines a novel mechanism for altering the timing of onset of sporulation. In addition, 6S-2 RNA does not influence the timing of sporulation, providing further evidence of the independent influences of these two related RNAs on cell physiology.

  12. The APSES protein Sok2 is a positive regulator of sporulation in Ashbya gossypii.

    Science.gov (United States)

    Wasserstrom, Lisa; Dünkler, Alexander; Walther, Andrea; Wendland, Jürgen

    2017-12-01

    Ashbya gossypii is a homothallic, flavinogenic, filamentous ascomycete that starts overproduction of riboflavin and fragments its mycelium quantitatively into spore producing sporangia at the end of a growth phase. Mating is not required for sporulation and the standard homothallic laboratory strain is a MATa strain. Here we show that ectopic expression of Saccharomyces cerevisiae MATα2 in A. gossypii completely suppresses sporulation, inhibits riboflavin overproduction and downregulates among others AgSOK2. AgSok2 belongs to a fungal-specific group of (APSES) transcription factors. Deletion of AgSOK2 strongly reduces riboflavin production and blocks sporulation. The initiator of meiosis, AgIME1, is a transcription factor essential for sporulation. We characterized the AgIME1 promoter region required for complementation of the Agime1 mutant. Reporter assays with AgIME1 promoter fragments fused to lacZ showed that AgSok2 does not control AgIME1 transcription. However, global transcriptome analysis identified two other essential regulators of sporulation, AgIME2 and AgNDT80, as potential targets of AgSok2. Our data suggest that sporulation and riboflavin production in A. gossypii are under mating type locus and nutritional control. Sok2, a target of the cAMP/protein kinase A pathway, serves as a central positive regulator to promote sporulation. This contrasts Saccharomyces cerevisiae where Sok2 is a repressor of IME1 transcription. © 2017 John Wiley & Sons Ltd.

  13. Selection of G1 Phase Yeast Cells for Synchronous Meiosis and Sporulation.

    Science.gov (United States)

    Stuart, David T

    2017-01-01

    Centrifugal elutriation is a procedure that allows the fractionation of cell populations based upon their size and shape. This allows cells in distinct cell cycle stages can be captured from an asynchronous population. The technique is particularly helpful when performing an experiment to monitor the progression of cells through the cell cycle or meiosis. Yeast sporulation like gametogenesis in other eukaryotes initiates from the G1 phase of the cell cycle. Conveniently, S. cerevisiae arrest in G1 phase when starved for nutrients and so withdrawal of nitrogen and glucose allows cells to abandon vegetative growth in G1 phase before initiating the sporulation program. This simple starvation protocol yields a partial synchronization that has been used extensively in studies of progression through meiosis and sporulation. By using centrifugal elutriation it is possible to isolate a homogeneous population of G1 phase cells and induce them to sporulate synchronously, which is beneficial for investigating progression through meiosis and sporulation. An additionally benefit of this protocol is that cell populations can be isolated based upon size and both large and small cell populations can be tested for progression through meiosis and sporulation. Here we present a protocol for purification of G1 phase diploid cells for examining synchronous progression through meiosis and sporulation.

  14. Differential effects of sporulation temperature on the high pressure resistance of Clostridium botulinum type E spores and the interconnection with sporulation medium cation contents.

    Science.gov (United States)

    Lenz, Christian A; Vogel, Rudi F

    2015-04-01

    High pressure thermal (HPT) processing can be used to improve traditional preservation methods and increase food safety and durability, whereas quality related characteristics can be largely maintained. Clostridium (C.) botulinum type E is a non-proteolytic, psychrotrophic, toxin-producing spore former, commonly associated with aquatic environments in temperate regions of the northern hemisphere. Sporulation in nature is likely to occur under varying conditions including temperature and nutrient availability, which might affect resistance properties of resulting spores. In our study, we determined the effect of sporulation temperature (13-38 °C) on the resistance of three Clostridium botulinum type E strains to differently intense HPT treatments (200 MPa at 40 and 80 °C, and 800 MPa at 40 and 80 °C). Furthermore, the effect of cations on sporulation temperature-mediated alterations in HHP resistance was investigated. Results indicate that low and high sporulation temperatures can increase and decrease sporal HPT resistance, respectively, in a treatment-dependent (pressure level, treatment temperature) manner, whereas the trends observed are largely unaffected by pressure dwells (1 s-10 min). Furthermore, results show that the cation content of the sporulation medium (Ca(2+), Mg(2+), Mn(2+)) marginally influences and partially counteracts effects on the HPT resistance of spores grown at low and elevated temperatures, respectively. This suggests that sporulation temperature and medium cations provoke changes in some common spore resistance structures. Sporulation conditions can markedly affect spore resistance properties and, thus, should be considered for the experimental setup of worst case studies aiming to evaluate the effectiveness of food processes in terms of the inactivation of C. botulinum type E spores. Copyright © 2014 Elsevier Ltd. All rights reserved.

  15. How the early sporulation sigma factor sigmaF delays the switch to late development in Bacillus subtilis.

    Science.gov (United States)

    Karmazyn-Campelli, Céline; Rhayat, Lamya; Carballido-López, Rut; Duperrier, Sandra; Frandsen, Niels; Stragier, Patrick

    2008-03-01

    Sporulation in Bacillus subtilis is a primitive differentiation process involving two cell types, the forespore and the mother cell. Each cell implements two successive transcription programmes controlled by specific sigma factors. We report that activity of sigma(G), the late forespore sigma factor, is kept in check by Gin, the product of csfB, a gene controlled by sigma(F), the early forespore sigma factor. Gin abolishes sigma(G) transcriptional activity when sigma(G) is artificially synthesized during growth, but has no effect on sigma(F). Gin interacts strongly with sigma(G) but not with sigma(F) in a yeast two-hybrid experiment. The absence of Gin allows sigma(G) to be active during sporulation independently of the mother-cell development to which it is normally coupled. Premature sigma(G) activity leads to the formation of slow-germinating spores, and complete deregulation of sigma(G) synthesis is lethal when combined with gin inactivation. Gin allows sigma(F) to delay the switch to the late forespore transcription programme by preventing sigma(G) to take over before the cell has reached a critical stage of development. A similar strategy, following a completely unrelated route, is used by the mother cell.

  16. Effects of microbial loading and sporulation temperature on atmospheric plasma inactivation of Bacillus subtilis spores

    Science.gov (United States)

    Deng, X. T.; Shi, J. J.; Shama, G.; Kong, M. G.

    2005-10-01

    Current inactivation studies of Bacillus subtilis spores using atmospheric-pressure glow discharges (APGD) do not consider two important factors, namely microbial loading at the surface of a substrate and sporulation temperature. Yet these are known to affect significantly microbial resistance to heat and hydrogen peroxide. This letter investigates effects of microbial loading and sporulation temperature on spore resistance to APGD. It is shown that microbial loading can lead to a stacking structure as a protective shield against APGD treatment and that high sporulation temperature increases spore resistance by altering core water content and cross-linked muramic acid content of B. subtilis spores.

  17. Radiation damage and its repair in non-sporulating bacteria

    International Nuclear Information System (INIS)

    Moseley, B.E.B.

    1984-01-01

    A review is given of radiation damage and its repair in non-sporulating bacteria. The identification and measurement of radiation damage in the DNA of the bacteria after exposure to ultraviolet radiation and ionizing radiation is described. Measuring the extent of DNA repair and ways of isolating repair mutants are also described. The DNA repair mechanisms for UV-induced damage are discussed including photoreactivation repair, excision repair, post-replication recombination repair and induced error-prone repair. The DNA repair mechanisms for ionizing radiation damage are also discussed including the repair of both single and double-strand breaks. Other aspects discussed include the effects of growth, irradiation medium and recovery medium on survival, DNA repair in humans, the commercial use of UV and ionizing radiations and the future of ionizing irradiation as a food treatment process. (U.K.)

  18. Connecting people to place

    NARCIS (Netherlands)

    Horlings, L.G.

    2016-01-01

    The article describes a process of preparing a research design on place-shaping, as outcome of a process of co-design between academic actors and non-academic actors in Brazil, South Africa and The Netherlands, taking place in the context of the project TRANSPLACE. The joint research design

  19. Identification of diacetonamine from soybean curd residue as a sporulation-inducing factor toward Bacillus spp.

    Science.gov (United States)

    Ikeda, Aki; Kim, Dongyeop; Hashidoko, Yasuyuki

    2017-12-01

    Under bioassay-guided investigation, a sporulation-inducing factor (SIF) toward Bacillus spp. was searched for in methanol (MeOH) extracts of soybean curd residues, and diacetonamine (1) was identified as the active compound. SIF was first isolated as a monoacetylated derivative (2, 4.1 mg from 655 g soybean curd residues), and its chemical structure was elucidated by field desorption mass spectrometry, electron ionization mass spectrometry, and nuclear magnetic resonance (NMR) analyses. After 48-h incubation, 40 µM diacetonamine hydrochloride (1b) exhibited sporulation-inducing activity with 35% sporulation frequency toward a Bacillus amyloliquefaciens wild-type strain (AHU 2170), whereas 40 µM diacetone acrylamide (3) showed 99% sporulation induction, which was much higher than that of 1b. Although Bacillus megaterium NBRC 15308 was sporulated by the treatment with 400 µM 1b with 36 and 70% sporulation frequency after 72- and 96-h incubation respectively, 3 at the same concentration showed only 2% sporulation after 72-h incubation. Hence, diacetonamine (1) was characterized as a genuine SIF from soybean curd residues, but it was uncertain whether 1 is a natural product or an artifact. Spores of B. amyloliquefaciens induced by 1b survived after treatment with heating at 95 °C for 10 min, also suggesting that 1 is genuine SIF in soybean curd residue. As sporulation induction is likely linked to activation of antibiotic production in some spore-forming Firmicutes bacteria, compound 1 would be a possible chemical tool to develop an effective fermentation technology in Bacillus species.

  20. Reconsidering the sporulation characteristics of hypervirulent Clostridium difficile BI/NAP1/027.

    Directory of Open Access Journals (Sweden)

    David A Burns

    Full Text Available Clostridium difficile is the leading cause of antibiotic-associated diarrhoea and a major burden to healthcare services worldwide. In recent years, C. difficile strains belonging to the BI/NAP1/027 type have become highly represented among clinical isolates. These so-called 'hypervirulent' strains are associated with outbreaks of increased disease severity, higher relapse rates and an expanded repertoire of antibiotic resistance. Spores, formed during sporulation, play a pivotal role in disease transmission and it has been suggested that BI/NAP1/027 strains are more prolific in terms of sporulation in vitro than 'non-epidemic' C. difficile types. Work in our laboratory has since provided credible evidence to the contrary suggesting that the strain-to-strain variation in C. difficile sporulation characteristics is not type-associated. However, the BI/NAP1/027 type is still widely stated to have an increased rate of sporulation. In this study, we analysed the sporulation rates of 53 C. difficile strains, the largest sample size used to-date in such a study, including 28 BI/NAP1/027 isolates. Our data confirm that significant variation exists in the rate at which different C. difficile strains form spores. However, we clearly show that the sporulation rate of the BI/NAP1/027 type was no higher than that of non-BI/NAP1/027 strains. In addition, we observed substantial variation in sporulation characteristics within the BI/NAP1/027 type. This work highlights the danger of assuming that all strains of one type behave similarly without studying adequate sample sizes. Furthermore, we stress the need for more rigorous experimental procedures in order to quantify C. difficile sporulation more accurately in the future.

  1. Increased Sporulation of Vesicular-Arbuscular Mycorrhizal Fungi by Manipulation of Nutrient Regimens †

    OpenAIRE

    Douds, David D.; Schenck, N. C.

    1990-01-01

    Adjustment of pot culture nutrient solutions increased root colonization and sporulation of vesicular-arbuscular mycorrhizal (VAM) fungi. Paspalum notatum Flugge and VAM fungi were grown in a sandy soil low in N and available P. Hoagland nutrient solution without P enhanced sporulation in soil and root colonization of Acaulospora longula, Scutellospora heterogama, Gigaspora margarita, and a wide range of other VAM fungi over levels produced by a tap water control or nutrient solutions contain...

  2. Children's Places

    DEFF Research Database (Denmark)

    Using a cross-cultural approach the book investigates children's places in different societies. "Children's Places" examines the ways in which children and adults, from their different vantage-points in society, negotiate proper places of children in both social and spatial terms. It looks at some...

  3. Small proteins link coat and cortex assembly during sporulation in Bacillus subtilis

    Science.gov (United States)

    Ebmeier, Sarah E.; Tan, Irene S.; Clapham, Katie Rose; Ramamurthi, Kumaran S.

    2015-01-01

    Summary Mature spores of the bacterium Bacillus subtilis are encased by two concentric shells: an inner shell (the ‘cortex’), made of peptidoglycan; and an outer proteinaceous shell (the ‘coat’), whose basement layer is anchored to the surface of the developing spore via a 26-amino-acid-long protein called SpoVM. During sporulation, initiation of cortex assembly depends on the successful initiation of coat assembly, but the mechanisms that co-ordinate the morphogenesis of both structures are largely unknown. Here, we describe a sporulation pathway involving SpoVM and a 37-amino-acid-long protein named ‘CmpA’ that is encoded by a previously un-annotated gene and is expressed under control of two sporulation-specific transcription factors (σE and SpoIIID). CmpA localized to the surface of the developing spore and deletion of cmpA resulted in cells progressing through the sporulation programme more quickly. Overproduction of CmpA did not affect normal growth or cell division, but delayed entry into sporulation and abrogated cortex assembly. In those cells that had successfully initiated coat assembly, CmpA was removed by a posttranslational mechanism, presumably in order to overcome the sporulation inhibition it imposed. We propose a model in which CmpA participates in a developmental checkpoint that ensures the proper orchestration of coat and cortex morphogenesis by repressing cortex assembly until coat assembly successfully initiates. PMID:22463703

  4. Metagenomic analysis of buffalo rumen microbiome: Effect of roughage diet on Dormancy and Sporulation genes.

    Science.gov (United States)

    Singh, K M; Reddy, B; Patel, A K; Panchasara, H; Parmar, N; Patel, A B; Shah, T M; Bhatt, V D; Joshi, C G

    2014-12-01

    Buffalo rumen microbiome experiences a variety of diet stress and represents reservoir of Dormancy and Sporulation genes. However, the information on genomic responses to such conditions is very limited. The Ion Torrent PGM next generation sequencing technology was used to characterize general microbial diversity and the repertoire of microbial genes present, including genes associated with Dormancy and Sporulation in Mehsani buffalo rumen metagenome. The research findings revealed the abundance of bacteria at the domain level and presence of Dormancy and Sporulation genes which were predominantly associated with the Clostridia and Bacilli taxa belonging to the phyla Firmicutes. Genes associated with Sporulation cluster and Sporulation orphans were increased from 50% to 100% roughage treatment, thereby promoting sporulation all along the treatments. The spore germination is observed to be the highest in the 75% roughage treatment both in the liquid and solid rumen fraction samples with respect to the decrease in the values of the genes associated with spore core dehydration, thereby facilitating spore core hydration which is necessary for spore germination.

  5. The effect of fermentable carbohydrate on sporulation and butanol production by Clostridium acetobutylicum P262

    Energy Technology Data Exchange (ETDEWEB)

    Awang, G.M.; Ingledew, W.M.; Jones, G.A. (Saskatchewan Univ., Saskatoon, SK (Canada). Dept. of Applied Microbiology and Food Science)

    1992-10-01

    This study was conducted to determine whether or not a variation in the type of carbohydrate fermented by Clostridium acetobutylicum could be exploited to inhibit sporulation during the butanol-producing phase of fermentation and thus enhance butanol production. C. acetobutylicum P262 was found to ferment a wide variety of carbohydrates, but butanol production was not necessarily enhanced when percentage sporulation was low. Butanol concentration was more related to the total amount of acidic end-products (acetic and butyric acid) reutilized by the microorganism for solvent production and to the type and amount of carbohydrate utilized. Fermentation of cellobiose led to conditions resulting in complete acid reutilization and the highest butanol concentration (10.4-10.6 g/l). In cultures containing a mixture of glucose and cellobiose, glucose repression of cellobiose utilization resulted in lower butanol concentrations (6.6-7.5 g/l). Sporulation was dependent on the type of carbohydrate utilized by the microorgamism. Glucose had a greater enhancing effect on the sporulation process (22-42%) than starch (9-12%) or cellobiose (22-34%). It was concluded that whereas the type of carbohydrate fermented has a specific effect on the extent of sporulation of a culture, conditions of low sporulation did not enhance butanol concentration unless carbohydrate utilization and the reutilization of acidic products were high. (orig.).

  6. Asexual sporulation facilitates adaptation: The emergence of azole resistance in Aspergillus fumigatus.

    Science.gov (United States)

    Zhang, Jianhua; Debets, Alfons J M; Verweij, Paul E; Melchers, Willem J G; Zwaan, Bas J; Schoustra, Sijmen E

    2015-10-01

    Understanding the occurrence and spread of azole resistance in Aspergillus fumigatus is crucial for public health. It has been hypothesized that asexual sporulation, which is abundant in nature, is essential for phenotypic expression of azole resistance mutations in A. fumigatus facilitating subsequent spread through natural selection. Furthermore, the disease aspergilloma is associated with asexual sporulation within the lungs of patients and the emergence of azole resistance. This study assessed the evolutionary advantage of asexual sporulation by growing the fungus under pressure of one of five different azole fungicides over seven weeks and by comparing the rate of adaptation between scenarios of culturing with and without asexual sporulation. Results unequivocally show that asexual sporulation facilitates adaptation. This can be explained by the combination of more effective selection because of the transition from a multicellular to a unicellular stage, and by increased mutation supply due to the production of spores, which involves numerous mitotic divisions. Insights from this study are essential to unravel the resistance mechanisms of sporulating pathogens to chemical compounds and disease agents in general, and for designing strategies that prevent or overcome the emerging threat of azole resistance in particular. © 2015 The Author(s). Evolution © 2015 The Society for the Study of Evolution.

  7. Energy and calcium ion dependence of proteolysis during sporulation of Bacillus subtilis cells

    International Nuclear Information System (INIS)

    O'Hara, M.B.; Hageman, J.H.

    1990-01-01

    The authors have shown, with an optimized [ 14 C]leucine-labeling and chasing procedure, that intracellular protein degradation in sporulating cells of Bacillus subtilis 168 (trpC2) is apparently energy dependent. Sodium arsenate, sodium azide, carbonyl cyanide m-chlorophenylhydrozone, and N,N'-dicyclohexylcarbodiimide, at levels which did not induce appreciable lysis (≤ 10%) over 10-h periods of sporulation, inhibited intracellular proteolysis by 13 to 93%. Exponentially growing cells acquired arsenate resistance. In contrast to earlier reports, the authors found that chloramphenicol strongly inhibited proteolysis even when added 6 h into the sporulation process. Restricting the calcium ion concentration in the medium had no effect on rates or extent of vegetative growth, strongly inhibited sporulation, and inhibited rates of proteolysis by 60% or more. Inhibitors of energy metabolism, at the same levels which inhibited proteolysis, did not affect the rate or degree of uptake of Ca 2+ by cells. Restricting the Ca 2+ concentration in the medium reduced by threefold of the specific activity in cells of the major intracellular serine proteinase after 12 h of sporulation. finally, cells of a mutant of B. subtilis bearing an insertionally inactivated gene for the Ca 2+ -dependent intracellular proteinase-1 degraded protein in chemically defined sporulation medium at a rate indistinguishable from that of the wild-type cells for period of 8 h

  8. Temperature and light intensity interaction on Cercospora coffeicola sporulation and conidia germination

    Directory of Open Access Journals (Sweden)

    Marília Goulart da Silva

    2016-04-01

    Full Text Available ABSTRACT Difficulty in obtaining abundant sporulation in culture of many species of Cercospora may be the limiting factor for studies of biology, systematics, and inoculation of the genus. Therefore, it is necessary to understand the nutritional and environmental requirements that influence mycelial growth, sporulation and germination. As it is difficult to obtain conidia of Cercospora coffeicola in vitro, different temperatures (17, 22, 27, and 32 °C and light intensities (80, 160, 240, and 320 μmol m-2 s-1 were evaluated to optimize pathogen sporulation and assess favorable conditions for spore germination, aiming for a strategy of disease control. The dark treatment (0 μmol m-2 s-1 was added for sporulation. A significant interaction was found between temperature and light intensity for both variables. The highest sporulation rate of C. coffeicola occurred at a light intensity of 240 μmol m-2 s-1 and air temperature of 22 °C, reaching 5.9x106 con mL-1. Germination was higher at temperature 17 °C and light intensity of 320 μmol m-2 s-1, reaching 52%. Interaction between light intensity and temperature proved to influence the processes of sporulation and germination of C. coffeicola.

  9. Four linked genes participate in controlling sporulation efficiency in budding yeast.

    Directory of Open Access Journals (Sweden)

    Giora Ben-Ari

    2006-11-01

    Full Text Available Quantitative traits are conditioned by several genetic determinants. Since such genes influence many important complex traits in various organisms, the identification of quantitative trait loci (QTLs is of major interest, but still encounters serious difficulties. We detected four linked genes within one QTL, which participate in controlling sporulation efficiency in Saccharomyces cerevisiae. Following the identification of single nucleotide polymorphisms by comparing the sequences of 145 genes between the parental strains SK1 and S288c, we analyzed the segregating progeny of the cross between them. Through reciprocal hemizygosity analysis, four genes, RAS2, PMS1, SWS2, and FKH2, located in a region of 60 kilobases on Chromosome 14, were found to be associated with sporulation efficiency. Three of the four "high" sporulation alleles are derived from the "low" sporulating strain. Two of these sporulation-related genes were verified through allele replacements. For RAS2, the causative variation was suggested to be a single nucleotide difference in the upstream region of the gene. This quantitative trait nucleotide accounts for sporulation variability among a set of ten closely related winery yeast strains. Our results provide a detailed view of genetic complexity in one "QTL region" that controls a quantitative trait and reports a single nucleotide polymorphism-trait association in wild strains. Moreover, these findings have implications on QTL identification in higher eukaryotes.

  10. Women take the island: nation, profession, place Women take the island: nation, profession, place

    Directory of Open Access Journals (Sweden)

    Ruth Morse

    2008-04-01

    Full Text Available The Tempest has been one of Shakespeare’s most adapted plays. Its stage history is concomitantly a history of the British theatre, from regularized comedy to semi-opera to pantomime to opera. It has had other lives, too, from its position in romantic ideas of Shakespeare’s biography and his so-called farewell to the stage, to a supporting role as witness for the nineteenth-century Darwinians’ idea of the missing link, to a veritable efflorescence of walk-on parts, cameos, and star vehicles in twentieth-century psychoanalytic and social arguments about European expansion.2 The play has given us individual poems and paintings, not to speak of screen-plays for several film adaptations. The Tempest has been one of Shakespeare’s most adapted plays. Its stage history is concomitantly a history of the British theatre, from regularized comedy to semi-opera to pantomime to opera. It has had other lives, too, from its position in romantic ideas of Shakespeare’s biography and his so-called farewell to the stage, to a supporting role as witness for the nineteenth-century Darwinians’ idea of the missing link, to a veritable efflorescence of walk-on parts, cameos, and star vehicles in twentieth-century psychoanalytic and social arguments about European expansion.2 The play has given us individual poems and paintings, not to speak of screen-plays for several film adaptations.

  11. σK of Clostridium acetobutylicum is the first known sporulation-specific sigma factor with two developmentally separated roles, one early and one late in sporulation.

    Science.gov (United States)

    Al-Hinai, Mohab A; Jones, Shawn W; Papoutsakis, Eleftherios T

    2014-01-01

    Sporulation in the model endospore-forming organism Bacillus subtilis proceeds via the sequential and stage-specific activation of the sporulation-specific sigma factors, σ(H) (early), σ(F), σ(E), σ(G), and σ(K) (late). Here we show that the Clostridium acetobutylicum σ(K) acts both early, prior to Spo0A expression, and late, past σ(G) activation, thus departing from the B. subtilis model. The C. acetobutylicum sigK deletion (ΔsigK) mutant was unable to sporulate, and solventogenesis, the characteristic stationary-phase phenomenon for this organism, was severely diminished. Transmission electron microscopy demonstrated that the ΔsigK mutant does not develop an asymmetric septum and produces no granulose. Complementation of sigK restored sporulation and solventogenesis to wild-type levels. Spo0A and σ(G) proteins were not detectable by Western analysis, while σ(F) protein levels were significantly reduced in the ΔsigK mutant. spo0A, sigF, sigE, sigG, spoIIE, and adhE1 transcript levels were all downregulated in the ΔsigK mutant, while those of the sigH transcript were unaffected during the exponential and transitional phases of culture. These data show that σ(K) is necessary for sporulation prior to spo0A expression. Plasmid-based expression of spo0A in the ΔsigK mutant from a nonnative promoter restored solventogenesis and the production of Spo0A, σ(F), σ(E), and σ(G), but not sporulation, which was blocked past the σ(G) stage of development, thus demonstrating that σ(K) is also necessary in late sporulation. sigK is expressed very early at low levels in exponential phase but is strongly upregulated during the middle to late stationary phase. This is the first sporulation-specific sigma factor shown to have two developmentally separated roles.

  12. Place Branding

    DEFF Research Database (Denmark)

    Medway, Dominic; Swanson, Kathryn; Neirotti, Lisa Delpy

    2015-01-01

    Purpose: – The purpose of this paper is to report on a special session entitled “Place branding: Are we wasting our time?”, held at the American Marketing Association’s Summer Marketing Educators’ conference in 2014. Design/methodology/approach: – The report details the outcome of an Oxford......: – The outcome of the debate points towards a need for place brands to develop as more inclusive and organic entities, in which case it may be best for place practitioners to avoid creating and imposing a place brand and instead help shape it from the views of stakeholder constituencies. This shifts the notion...... of place branding towards an activity centred on “curation”. Originality/value: – The use of a competitive debating format as a means for exploring academic ideas and concepts in the place management field....

  13. Computational modelling and analysis of the molecular network regulating sporulation initiation in Bacillus subtilis.

    Science.gov (United States)

    Ihekwaba, Adaoha E C; Mura, Ivan; Barker, Gary C

    2014-10-24

    Bacterial spores are important contaminants in food, and the spore forming bacteria are often implicated in food safety and food quality considerations. Spore formation is a complex developmental process involving the expression of more than 500 genes over the course of 6 to 8 hrs. The process culminates in the formation of resting cells capable of resisting environmental extremes and remaining dormant for long periods of time, germinating when conditions promote further vegetative growth. Experimental observations of sporulation and germination are problematic and time consuming so that reliable models are an invaluable asset in terms of prediction and risk assessment. In this report we develop a model which assists in the interpretation of sporulation dynamics. This paper defines and analyses a mathematical model for the network regulating Bacillus subtilis sporulation initiation, from sensing of sporulation signals down to the activation of the early genes under control of the master regulator Spo0A. Our model summarises and extends other published modelling studies, by allowing the user to execute sporulation initiation in a scenario where Isopropyl β-D-1-thiogalactopyranoside (IPTG) is used as an artificial sporulation initiator as well as in modelling the induction of sporulation in wild-type cells. The analysis of the model results and the comparison with experimental data indicate that the model is good at predicting inducible responses to sporulation signals. However, the model is unable to reproduce experimentally observed accumulation of phosphorelay sporulation proteins in wild type B. subtilis. This model also highlights that the phosphorelay sub-component, which relays the signals detected by the sensor kinases to the master regulator Spo0A, is crucial in determining the response dynamics of the system. We show that there is a complex connectivity between the phosphorelay features and the master regulatory Spo0A. Additional we discovered that the

  14. Constitutive expression of ftsZ overrides the whi developmental genes to initiate sporulation of Streptomyces coelicolor.

    Science.gov (United States)

    Willemse, Joost; Mommaas, A Mieke; van Wezel, Gilles P

    2012-03-01

    The filamentous soil bacteria Streptomyces undergo a highly complex developmental programme. Before streptomycetes commit themselves to sporulation, distinct morphological checkpoints are passed in the aerial hyphae that are subject to multi-level control by the whi sporulation genes. Here we show that whi-independent expression of FtsZ restores sporulation to the early sporulation mutants whiA, whiB, whiG, whiH, whiI and whiJ. Viability, stress resistance and high-resolution electron microscopy underlined that viable spores were formed. However, spores from sporulation-restored whiA and whiG mutants showed defects in DNA segregation/condensation, while spores from the complemented whiB mutant had increased stress sensitivity, perhaps as a result of changes in the spore sheath. In contrast to the whi mutants, normal sporulation of ssgB null mutants-which fail to properly localise FtsZ-could not be restored by enhancing FtsZ protein levels, forming spore-like bodies that lack spore walls. Our data strongly suggest that the whi genes control a decisive event towards sporulation of streptomycetes, namely the correct timing of developmental ftsZ transcription. The biological significance may be to ensure that sporulation-specific cell division will only start once sufficient aerial mycelium biomass has been generated. Our data shed new light on the longstanding question as to how whi genes control sporulation, which has intrigued scientists for four decades.

  15. Myb transcription factors and light regulate sporulation in the oomycete Phytophthora infestans.

    Science.gov (United States)

    Xiang, Qijun; Judelson, Howard S

    2014-01-01

    Life cycle progression in eukaryotic microbes is often influenced by environment. In the oomycete Phytophthora infestans, which causes late blight on potato and tomato, sporangia have been reported to form mostly at night. By growing P. infestans under different light regimes at constant temperature and humidity, we show that light contributes to the natural pattern of sporulation by delaying sporulation until the following dark period. However, illumination does not permanently block sporulation or strongly affect the total number of sporangia that ultimately form. Based on measurements of sporulation-induced genes such as those encoding protein kinase Pks1 and Myb transcription factors Myb2R1 and Myb2R3, it appears that most spore-associated transcripts start to rise four to eight hours before sporangia appear. Their mRNA levels oscillate with the light/dark cycle and increase with the amount of sporangia. An exception to this pattern of expression is Myb2R4, which is induced several hours before the other genes and declines after cultures start to sporulate. Transformants over-expressing Myb2R4 produce twice the number of sporangia and ten-fold higher levels of Myb2R1 mRNA than wild-type, and chromatin immunoprecipitation showed that Myb2R4 binds the Myb2R1 promoter in vivo. Myb2R4 thus appears to be an early regulator of sporulation. We attempted to silence eight Myb genes by DNA-directed RNAi, but succeeded only with Myb2R3, which resulted in suppressed sporulation. Ectopic expression studies of seven Myb genes revealed that over-expression frequently impaired vegetative growth, and in the case of Myb3R6 interfered with sporangia dormancy. We observed that the degree of silencing induced by a hairpin construct was correlated with its copy number, and ectopic expression was often unstable due to epigenetic silencing and transgene excision.

  16. Myb transcription factors and light regulate sporulation in the oomycete Phytophthora infestans.

    Directory of Open Access Journals (Sweden)

    Qijun Xiang

    Full Text Available Life cycle progression in eukaryotic microbes is often influenced by environment. In the oomycete Phytophthora infestans, which causes late blight on potato and tomato, sporangia have been reported to form mostly at night. By growing P. infestans under different light regimes at constant temperature and humidity, we show that light contributes to the natural pattern of sporulation by delaying sporulation until the following dark period. However, illumination does not permanently block sporulation or strongly affect the total number of sporangia that ultimately form. Based on measurements of sporulation-induced genes such as those encoding protein kinase Pks1 and Myb transcription factors Myb2R1 and Myb2R3, it appears that most spore-associated transcripts start to rise four to eight hours before sporangia appear. Their mRNA levels oscillate with the light/dark cycle and increase with the amount of sporangia. An exception to this pattern of expression is Myb2R4, which is induced several hours before the other genes and declines after cultures start to sporulate. Transformants over-expressing Myb2R4 produce twice the number of sporangia and ten-fold higher levels of Myb2R1 mRNA than wild-type, and chromatin immunoprecipitation showed that Myb2R4 binds the Myb2R1 promoter in vivo. Myb2R4 thus appears to be an early regulator of sporulation. We attempted to silence eight Myb genes by DNA-directed RNAi, but succeeded only with Myb2R3, which resulted in suppressed sporulation. Ectopic expression studies of seven Myb genes revealed that over-expression frequently impaired vegetative growth, and in the case of Myb3R6 interfered with sporangia dormancy. We observed that the degree of silencing induced by a hairpin construct was correlated with its copy number, and ectopic expression was often unstable due to epigenetic silencing and transgene excision.

  17. The Phosphotransfer Protein CD1492 Represses Sporulation Initiation in Clostridium difficile.

    Science.gov (United States)

    Childress, Kevin O; Edwards, Adrianne N; Nawrocki, Kathryn L; Anderson, Sarah E; Woods, Emily C; McBride, Shonna M

    2016-12-01

    The formation of spores is critical for the survival of Clostridium difficile outside the host gastrointestinal tract. Persistence of C. difficile spores greatly contributes to the spread of C. difficile infection (CDI), and the resistance of spores to antimicrobials facilitates the relapse of infection. Despite the importance of sporulation to C. difficile pathogenesis, the molecular mechanisms controlling spore formation are not well understood. The initiation of sporulation is known to be regulated through activation of the conserved transcription factor Spo0A. Multiple regulators influence Spo0A activation in other species; however, many of these factors are not conserved in C. difficile and few novel factors have been identified. Here, we investigated the function of a protein, CD1492, that is annotated as a kinase and was originally proposed to promote sporulation by directly phosphorylating Spo0A. We found that deletion of CD1492 resulted in increased sporulation, indicating that CD1492 is a negative regulator of sporulation. Accordingly, we observed increased transcription of Spo0A-dependent genes in the CD1492 mutant. Deletion of CD1492 also resulted in decreased toxin production in vitro and in decreased virulence in the hamster model of CDI. Further, the CD1492 mutant demonstrated effects on gene expression that are not associated with Spo0A activation, including lower sigD and rstA transcription, suggesting that this protein interacts with factors other than Spo0A. Altogether, the data indicate that CD1492 negatively affects sporulation and positively influences motility and virulence. These results provide further evidence that C. difficile sporulation is regulated differently from that of other endospore-forming species. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  18. Better Place

    DEFF Research Database (Denmark)

    Rask, Morten; Bakke, Nikolas; Lindhøj, Jan

    Better Place is trying to reshape the automotive industry by shifting transportation from a dependency on oil to a reliance on environmentally friendly renewable energy. Better Place is developing an extensive infrastructure system that will utilise overcapacity in the production of wind power...... among others and that will drive the global transportation industry to becoming driven by electric vehicles (EVs). Better Place does this by selling its customers 'mileage' and a car without a battery. The case highlights the internationalisation process of Better Place from an international business...... perspective in order to encourage a discussion and debate about how Better Place can make their grand vision a reality in the future by overcoming the obstacles that historically have been challenging the rise of the EV industry. The case includes a historical background of the EV industry by using Denmark...

  19. Healthy Places

    Centers for Disease Control (CDC) Podcasts

    Every person has a stake in environmental public health. As the environment deteriorates, so does the physical and mental health of the people within it. Healthy places are those designed and built to improve the quality of life for all people who live, work, worship, learn, and play within their borders -- where every person is free to make choices amid a variety of healthy, available, accessible, and affordable options. The CDC recognizes significant health issues and places that are vital in developing the Healthy Places program and provides examples in this report.

  20. CodY Promotes Sporulation and Enterotoxin Production by Clostridium perfringens Type A Strain SM101.

    Science.gov (United States)

    Li, Jihong; Freedman, John C; Evans, Daniel R; McClane, Bruce A

    2017-03-01

    Clostridium perfringens type D strains cause enterotoxemia and enteritis in livestock via epsilon toxin production. In type D strain CN3718, CodY was previously shown to increase the level of epsilon toxin production and repress sporulation. C. perfringens type A strains producing C. perfringens enterotoxin (CPE) cause human food poisoning and antibiotic-associated diarrhea. Sporulation is critical for C. perfringens type A food poisoning since spores contribute to transmission and resistance in the harsh food environment and sporulation is essential for CPE production. Therefore, the current study asked whether CodY also regulates sporulation and CPE production in SM101, a derivative of C. perfringens type A food-poisoning strain NCTC8798. An isogenic codY -null mutant of SM101 showed decreased levels of spore formation, along with lower levels of CPE production. A complemented strain recovered wild-type levels of both sporulation and CPE production. When this result was coupled with the earlier results obtained with CN3718, it became apparent that CodY regulation of sporulation varies among different C. perfringens strains. Results from quantitative reverse transcriptase PCR analysis clearly demonstrated that, during sporulation, codY transcript levels remained high in SM101 but rapidly declined in CN3718. In addition, abrB gene expression patterns varied significantly between codY -null mutants of SM101 and CN3718. Compared to the levels in their wild-type parents, the level of abrB gene expression decreased in the CN3718 codY -null mutant strain but significantly increased in the SM101 codY -null mutant strain, demonstrating CodY-dependent regulation differences in abrB expression between these two strains. This difference appears to be important since overexpression of the abrB gene in SM101 reduced the levels of sporulation and enterotoxin production, supporting the involvement of AbrB repression in regulating C. perfringens sporulation. Copyright © 2017

  1. Stage-specific fluorescence intensity of GFP and mCherry during sporulation In Bacillus Subtilis

    Directory of Open Access Journals (Sweden)

    Bailey Kirra

    2010-11-01

    Full Text Available Abstract Background Fluorescent proteins are powerful molecular biology tools that have been used to study the subcellular dynamics of proteins within live cells for well over a decade. Two fluorescent proteins commonly used to enable dual protein labelling are GFP (green and mCherry (red. Sporulation in the Gram positive bacterium Bacillus subtilis has been studied for many years as a paradigm for understanding the molecular basis for differential gene expression. As sporulation initiates, cells undergo an asymmetric division leading to differential gene expression in the small prespore and large mother cell compartments. Use of two fluorescent protein reporters permits time resolved examination of differential gene expression either in the same compartments or between compartments. Due to the spectral properties of GFP and mCherry, they are considered an ideal combination for co-localisation and co-expression experiments. They can also be used in combination with fluorescent DNA stains such as DAPI to correlate protein localisation patterns with the developmental stage of sporulation which can be linked to well characterised changes in DNA staining patterns. Findings While observing the recruitment of the transcription machinery into the forespore of sporulating Bacillus subtilis, we noticed the occurrence of stage-specific fluorescence intensity differences between GFP and mCherry. During vegetative growth and the initial stages of sporulation, fluorescence from both GFP and mCherry fusions behaved similarly. During stage II-III of sporulation we found that mCherry fluorescence was considerably diminished, whilst GFP signals remained clearly visible. This fluorescence pattern reversed during the final stage of sporulation with strong mCherry and low GFP fluorescence. These trends were observed in reciprocal tagging experiments indicating a direct effect of sporulation on fluorescent protein fluorophores. Conclusions Great care should be taken

  2. Place (National)

    Data.gov (United States)

    Department of Transportation — This map layer includes cities and towns in the United States, Puerto Rico, and the U.S. Virgin Islands (NTAD). A city or town is a place with a recorded population,...

  3. Modelling the sporulation of some fungi associated with cheese, at different temperature and water activity regimes.

    Science.gov (United States)

    Camardo Leggieri, Marco; Decontardi, Simone; Battilani, Paola

    2018-08-02

    The objectives of this study were to determine, in-vitro, the influence of temperature (T; 10-30 °C, step 5°), water activity (a w , 0.83-0.99; step 0.04) and time on sporulation (SPO) of some cheese-related fungi belonging to Penicillium spp. and A. versicolor. Overall, sporulation started rapidly (8 h in optimal conditions); it was significantly influenced by T and a w and the fungi studied were clearly distinguished based on their thermo-hydro adaptation. Boundary conditions for sporulation were defined for all the fungi considered and the sporulation rate was successfully modelled, especially based on T and time regimes. Penicillium crustosum, P. nordicum and P. verrucosum showed optimum for SPO at T between 20 and 25 °C and their sporulation continued up to a w  = 0.87 (a w  = 0.83 for P. nordicum). They resulted the fungi best adapted to the environmental conditions of ripening grana cheese storehouses; therefore, it is expected they dominate on the grana cheese surface. Studies on cheese are necessary to validate these results obtained on artificial media and without fungi co-occurrence. Copyright © 2018 Elsevier B.V. All rights reserved.

  4. Morphogenic Protein RodZ Interacts with Sporulation Specific SpoIIE in Bacillus subtilis.

    Science.gov (United States)

    Muchová, Katarína; Chromiková, Zuzana; Bradshaw, Niels; Wilkinson, Anthony J; Barák, Imrich

    2016-01-01

    The first landmark in sporulation of Bacillus subtilis is the formation of an asymmetric septum followed by selective activation of the transcription factor σF in the resulting smaller cell. How the morphological transformations that occur during sporulation are coupled to cell-specific activation of transcription is largely unknown. The membrane protein SpoIIE is a constituent of the asymmetric sporulation septum and is a crucial determinant of σF activation. Here we report that the morphogenic protein, RodZ, which is essential for cell shape determination, is additionally required for asymmetric septum formation and sporulation. In cells depleted of RodZ, formation of asymmetric septa is disturbed and σF activation is perturbed. During sporulation, we found that SpoIIE recruits RodZ to the asymmetric septum. Moreover, we detected a direct interaction between SpoIIE and RodZ in vitro and in vivo, indicating that SpoIIE-RodZ may form a complex to coordinate asymmetric septum formation and σF activation. We propose that RodZ could provide a link between the cell shape machinery and the coordinated morphological and developmental transitions required to form a resistant spore.

  5. A novel regulator controls Clostridium difficile sporulation, motility and toxin production.

    Science.gov (United States)

    Edwards, Adrianne N; Tamayo, Rita; McBride, Shonna M

    2016-06-01

    Clostridium difficile is an anaerobic pathogen that forms spores which promote survival in the environment and transmission to new hosts. The regulatory pathways by which C. difficile initiates spore formation are poorly understood. We identified two factors with limited similarity to the Rap sporulation proteins of other spore-forming bacteria. In this study, we show that disruption of the gene CD3668 reduces sporulation and increases toxin production and motility. This mutant was more virulent and exhibited increased toxin gene expression in the hamster model of infection. Based on these phenotypes, we have renamed this locus rstA, for regulator of sporulation and toxins. Our data demonstrate that RstA is a bifunctional protein that upregulates sporulation through an unidentified pathway and represses motility and toxin production by influencing sigD transcription. Conserved RstA orthologs are present in other pathogenic and industrial Clostridium species and may represent a key regulatory protein controlling clostridial sporulation. © 2016 John Wiley & Sons Ltd.

  6. In vitro activity of natural and chemical products on sporulation of Eimeria species oocysts of chickens.

    Science.gov (United States)

    Gadelhaq, Sahar M; Arafa, Waleed M; Abolhadid, Shawky M

    2018-02-15

    This study was designed to investigate the ability of two herbal extracts and different chemical substances to inhibit or disrupt sporulation of Eimeria species oocysts of the chickens. The two herbal extracts were Allium sativum (garlic) and Moringa olifiera while the chemical substances included commercial disinfectants and diclazuril. Field isolates of Eimeria oocysts were propagated in chickens to obtain a continuous source of oocysts. The collected unsporulated oocysts (10 5 oocysts/5 ml) were dispensed into 5 cm Petri dish. Three replicates were used for each treatment. The treated oocysts were incubated for 48 h at 25-29 °C and 80% relative humidity. The results showed that herbal extracts, the commercial recommended dose of Dettol, TH4, Phenol, Virkon ® S, and Diclazuril 20% have no effect on the sporulation. While Sodium hypochlorite showed a significant degree of sporulation inhibition reached to 49.67%. Moreover, 70% ethanol, and 10% formalin showed 100% sporulation inhibition. It was concluded that 70% ethanol and 10% formalin are the most effective methods to inhibit Eimeria species sporulation. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. Taking antacids

    Science.gov (United States)

    Heartburn - antacids; Reflux - antacids; GERD - antacids ... Antacids are a good treatment for heartburn that occurs once in a while. Take antacids about 1 hour after eating or when you have heartburn. If you are taking ...

  8. Sporulation properties and antimicrobial susceptibility in endemic and rare Clostridium difficile PCR ribotypes.

    Science.gov (United States)

    Zidaric, Valerija; Rupnik, Maja

    2016-06-01

    Increased sporulation and antibiotic resistance have been proposed to be associated with certain Clostridium difficile epidemic strains such as PCR ribotype 027. In this study we examined these properties in another widespread PCR ribotype, 014/020, in comparison to prevalent PCR ribotype 002 and a group of rarely represented PCR ribotypes. Highest sporulation was observed in 014/020 strains at 24 h, while after 72 h PCR ribotype 002 and rare PCR ribotypes formed higher total number of spores. PCR ribotype 014/020 strains exhibited slightly higher resistance to tested antimicrobials, followed by group of rare PCR ribotypes and less common PCR ribotype 002. Neither sporulation properties nor antibiotic resistance clearly differed in endemic and rare strains. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Sporulation of Bacillus spp. within biofilms: a potential source of contamination in food processing environments.

    Science.gov (United States)

    Faille, C; Bénézech, T; Midelet-Bourdin, G; Lequette, Y; Clarisse, M; Ronse, G; Ronse, A; Slomianny, C

    2014-06-01

    Bacillus strains are often isolated from biofilms in the food industries. Previous works have demonstrated that sporulation could occur in biofilms, suggesting that biofilms would be a significant source of food contamination with spores. In this study, we investigated the properties of mono-species and mixed Bacillus biofilms and the ability of Bacillus strains to sporulate inside biofilms. Bacillus strains were able to form mono-species biofilms on stainless steel coupons, with up to 90% spores after a 48 h-incubation. These spores were highly resistant to cleaning but were easily transferred to agar, mimicking the cross-contamination of food, thereby suggesting that biofilms would be of particular concern due to a potential for Bacillus spore food contamination. This hypothesis was strengthened by the fact that Bacillus strains were able to form mixed biofilms with resident strains and that sporulation still occurred easily in these complex structures. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. Increased sporulation of vesicular-arbuscular mycorrhizal fungi by manipulation of nutrient regimens.

    Science.gov (United States)

    Douds, D D; Schenck, N C

    1990-02-01

    Adjustment of pot culture nutrient solutions increased root colonization and sporulation of vesicular-arbuscular mycorrhizal (VAM) fungi. Paspalum notatum Flugge and VAM fungi were grown in a sandy soil low in N and available P. Hoagland nutrient solution without P enhanced sporulation in soil and root colonization of Acaulospora longula, Scutellospora heterogama, Gigaspora margarita, and a wide range of other VAM fungi over levels produced by a tap water control or nutrient solutions containing P. However, Glomus intraradices produced significantly more spores in plant roots in the tap water control treatment. The effect of the nutrient solutions was not due solely to N nutrition, because the addition of NH(4)NO(3) decreased both colonization and sporulation by G. margarita relative to levels produced by Hoagland solution without P.

  11. Poly(3-hydroxybutyrate) fuels the tricarboxylic acid cycle and de novo lipid biosynthesis during Bacillus anthracis sporulation.

    Science.gov (United States)

    Sadykov, Marat R; Ahn, Jong-Sam; Widhelm, Todd J; Eckrich, Valerie M; Endres, Jennifer L; Driks, Adam; Rutkowski, Gregory E; Wingerd, Kevin L; Bayles, Kenneth W

    2017-06-01

    Numerous bacteria accumulate poly(3-hydroxybutyrate) (PHB) as an intracellular reservoir of carbon and energy in response to imbalanced nutritional conditions. In Bacillus spp., where PHB biosynthesis precedes the formation of the dormant cell type called the spore (sporulation), the direct link between PHB accumulation and efficiency of sporulation was observed in multiple studies. Although the idea of PHB as an intracellular carbon and energy source fueling sporulation was proposed several decades ago, the mechanisms underlying PHB contribution to sporulation have not been defined. Here, we demonstrate that PHB deficiency impairs Bacillus anthracis sporulation through diminishing the energy status of the cells and by reducing carbon flux into the tricarboxylic acid (TCA) cycle and de novo lipid biosynthesis. Consequently, this metabolic imbalance decreased biosynthesis of the critical components required for spore integrity and resistance, such as dipicolinic acid (DPA) and the spore's inner membrane. Supplementation of the PHB deficient mutant with exogenous fatty acids overcame these sporulation defects, highlighting the importance of the TCA cycle and lipid biosynthesis during sporulation. Combined, the results of this work reveal the molecular mechanisms of PHB contribution to B. anthracis sporulation and provide valuable insight into the metabolic requirements for this developmental process in Bacillus species. © 2017 John Wiley & Sons Ltd.

  12. The sporulation of the green alga Ulva prolifera is controlled by changes in photosynthetic electron transport chain.

    Science.gov (United States)

    Wang, Hui; Lin, Apeng; Gu, Wenhui; Huan, Li; Gao, Shan; Wang, Guangce

    2016-04-22

    Sporulation and spore release are essential phases of the life cycle in algae and land plants. Ulva prolifera, which is an ideal organism for studying sporulation and spore release, was used as the experimental material in the present study. The determination of photosynthetic parameters, combined with microscopic observation, treatment with photosynthetic inhibitors, limitation of carbon acquisition, and protein mass spectrometry, was employed in this experiment. Cycle electron transport (CEF) was found enhanced at the onset of sporangia formation. The inhibition effect of dibromothymoquinone (DBMIB) towards sporulation was always strong during the sporulation process whereas the inhibition effect of 3-(3',4'-dichlorophenyl)-1,1-dimethylurea (DCMU) was continuously declined accompanied with the progress of sporulation. The changes of photosynthesis resulted from the limitation of CO2 acquisition could stimulate sporulation onset. Quantitative protein analysis showed that enzymes involved in carbon fixation, including RUBISCO and pyruvate orthophosphate dikinase, declined during sporogenesis, while proteins involved in sporulation, including tubulin and centrin, increased. These results suggest that enhanced cyclic electron flow (CEF) and oxidation of the plastoquinone pool are essential for sporangia formation onset, and changes in photosynthetic electron transport chain have significant impacts on sporulation of the green algae.

  13. Growth and sporulation of Bacillus cereus ATCC 14579 under defined conditions: temporal expression of genes for key sigma factors

    NARCIS (Netherlands)

    Vries, de Y.P.; Hornstra, L.M.; Vos, de W.M.; Abee, T.

    2004-01-01

    An airlift fermentor system allowing precise regulation of pH and aeration combined with a chemically defined medium was used to study growth and sporulation of Bacillus cereus ATCC 14579. Sporulation was complete and synchronous. Expression of sigA, sigB, sigF, and sigG was monitored with real-time

  14. Healthy Places

    Centers for Disease Control (CDC) Podcasts

    2007-04-10

    Every person has a stake in environmental public health. As the environment deteriorates, so does the physical and mental health of the people within it. Healthy places are those designed and built to improve the quality of life for all people who live, work, worship, learn, and play within their borders -- where every person is free to make choices amid a variety of healthy, available, accessible, and affordable options. The CDC recognizes significant health issues and places that are vital in developing the Healthy Places program and provides examples in this report.  Created: 4/10/2007 by CDC National Center for Environmental Health.   Date Released: 4/13/2007.

  15. Designed Places

    DEFF Research Database (Denmark)

    Stender, Marie

    The 2008 financial crisis has left traces in the built environment of Copenhagen like many other places: Building projects are left unfinished or their function or finish is changed due to new economic circumstances. An ethnographic exploration of these traces exposes central aspects of what is a......, and when the ceilings leak water, the residents suspect it to be a consequence of the crisis. The paper discusses how market forces interact with the material surroundings we inhabit and explores the relationship between controlled and uncontrollable in the design of places....

  16. Developmental changes in translatable RNA species and protein synthesis during sporulation in the aquatic fungus Blastocladiella emersonii

    International Nuclear Information System (INIS)

    Silva, A.M. da; Costa Maia, J.C. da; Juliani, M.H.

    1986-01-01

    Protein synthesis during sporulation in Blastocladiella emersonii is developmentally regulated as revealed using ( 35 S)methionine pulse labeling and two-dimensional gel electrophoresis. A large increase in the synthesis of several proteins is associated with particular stages. A large number of basic proteins are synthesized exclusively during late sporulation. Changes in translatable mRNA species were also detected by two-dimensional gel electrophoresis of the polypeptides produced in a cell-free rabbit reticulocyte lysate primed with RNA prepared at different stages of sporulation. The synthesis of several proteins during sporulation seems to be transcriptionally controlled. Most of the sporulation-specific messages are not present in the mature zoospores. (Author)

  17. Secret Places.

    Science.gov (United States)

    Ridolfi, Kerry

    1997-01-01

    Argues that children are as deep as the ocean, with secret places inside of them waiting to be opened. Notes that it is powerful for students to learn they can make sense of the world through words, and describes inviting them into poetry as they read poetry, create poetry packets, and write and revise poems. (SR)

  18. Envisioning place

    DEFF Research Database (Denmark)

    Schmidt, Garbi; Glick Schiller, Nina

    2016-01-01

    together, the articles contribute to an emerging relational social science by approaching urban sociabilities through four interrelated parameters: (1) a concept of place-making situated within trajectories of differential and multiscalar power; (2) a discursive analysis of narratives and silences...

  19. Growth and sporulation of Bacillus subtilis under microgravity (7-IML-1)

    Science.gov (United States)

    Mennigmann, Horst-Dieter

    1992-01-01

    The experiment was aimed at measuring the growth and sporulation of Bacillus subtilis under microgravity. The hardware for the experiment consists of a culture chamber (15 ml) made from titanium and closed by a membrane permeable for gases but not for water. Two variants of this basic structure were built which fit into the standard Biorack container types 1 and 2 respectively. Growth of the bacteria will be monitored by continuously measuring the optical density with a built-in miniaturized photometer. Other parameters (viability, sporulation, fine structure, size distribution of cells and spores, growth kinetics, etc.) will be measured on the fixed samples and on those where metabolism was temporarily halted, respectively.

  20. The effect of sporulation medium on Alicyclobacillus acidoterrestris guaiacol production in apple juice

    OpenAIRE

    Molva, Çelenk; Baysal, Ayşe Handan

    2016-01-01

    The present study evaluated the effect of sporulation medium on guaiacol formation from vanillin and vanillic acid by Alicyclobacillus acidoterrestris DSM 3922 in the reconstituted apple juice (pH 3.82, °Brix 11.3). For sporulation, potato dextrose agar and Bacillus acidoterrestris agar were used. After heat-activation, spores were turned into vegetative cells and inoculated into juice samples to a final concentration of 103 or 105 CFU/mL. Samples were incubated at 37 °C for 264 h and guaiaco...

  1. A Dual Role for the Bacillus anthracis Master Virulence Regulator AtxA: Control of Sporulation and Anthrax Toxin Production.

    Science.gov (United States)

    Dale, Jennifer L; Raynor, Malik J; Ty, Maureen C; Hadjifrangiskou, Maria; Koehler, Theresa M

    2018-01-01

    Bacillus anthracis is an endemic soil bacterium that exhibits two different lifestyles. In the soil environment, B. anthracis undergoes a cycle of saprophytic growth, sporulation, and germination. In mammalian hosts, the pathogenic lifestyle of B. anthracis is spore germination followed by vegetative cell replication, but cells do not sporulate. During infection, and in specific culture conditions, transcription of the structural genes for the anthrax toxin proteins and the biosynthetic operon for capsule synthesis is positively controlled by the regulatory protein AtxA. A critical role for the atxA gene in B. anthracis virulence has been established. Here we report an inverse relationship between toxin production and sporulation that is linked to AtxA levels. During culture in conditions favoring sporulation, B. anthracis produces little to no AtxA. When B. anthracis is cultured in conditions favoring toxin gene expression, AtxA is expressed at relatively high levels and sporulation rate and efficiency are reduced. We found that a mutation within the atxA promoter region resulting in AtxA over-expression leads to a marked sporulation defect. The sporulation phenotype of the mutant is dependent upon pXO2-0075 , an atxA -regulated open reading frame located on virulence plasmid pXO2. The predicted amino acid sequence of the pXO2-0075 protein has similarity to the sensor domain of sporulation sensor histidine kinases. It was shown previously that pXO2-0075 overexpression suppresses sporulation. We have designated pXO2-0075 " skiA " for "sporulation kinase inhibitor." Our results indicate that in addition to serving as a positive regulator of virulence gene expression, AtxA modulates B. anthracis development.

  2. Places Connected:

    DEFF Research Database (Denmark)

    Hansen, Annette Skovsted

    This paper argues that development assistance contributed to the globalization of the 20th century by financing truly global networks of people. By focusing on the networks financed by development assistance bound by the national histories of Denmark and Japan, I illustrate how the people who...... experiences of place, however, when it is often the same people who experience many different places? Along with many other so-called donors in the 1950s, Denmark and Japan chose to invest in the education of own and other nationals involved in development and thereby financed personal connections between...... individuals throughout the world. Development assistance , where there are two or three links only between a Bangladeshi farmer, a street child in Sao Paolo and the President of the United States, the Queen of Denmark, or a suburban house wife in Japan, who has never left the Osaka area, but mothered a United...

  3. Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: ACCESS 2000 - niveau 1 : 13 & 14.11.03 (2 jours) C++ for Particle Physicists : 17 – 21.11.03 (6 X 3-hour lectures) Programmation automate Schneider TSX Premium – niveau 2 : 18 – 21.11.03 (4 jours) JAVA 2 Enterprise Edition – Part 1 : WEB Applications : 20 & ...

  4. Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Places available The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses : Introduction à Outlook : 19.8.2004 (1 journée) Outlook (short course I) : E-mail : 31.8.2004 (2 hours, morning) Outlook (short course II) : Calendar, Tasks and Notes : 31.8.2004 (2 hours, afternoon) Instructor-led WBTechT Study or Follow-up for Microsoft Applications : 7.9.2004 (morning) Outlook (short course III) : Meetings and Delegation : 7.9.2004 (2 hours, afternoon) Introduction ...

  5. Places disponibles*/Places available **

    CERN Multimedia

    2003-01-01

    Des places sont disponibles dans les cours suivants : Places are available in the following course : Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 20 - 22.1.03 (3 days) Introduction to PVSS : 27.1.03 (Afternoon) free course but registration necessary Basic PVSS : 28 - 30.1.03 (3 days) MAGNE-03 - Magnétisme pour l'électrotechnique : 28 - 30.1.03 (3 jours) MAGNE-03 - Magnetism for Technical Electronics : 11 - 13.2.03 (3 days) AutoCAD 2002 - niveau 1 : 24, 25.2 et 3, 4.3.03 (4 jours) AutoCAD 2002 - niveau 2 : 10 & 11.3.03 (2 jours) C++ for Particle Physicists : 10 - 14.3.03 (6 X 3 hour lectures) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 17, 18, 24 & 25.3.03 (6 jours) * Etant donné le délai d'impression du Bulletin, ces places peuvent ne plus être disponibles au moment de sa parution. Veuillez consulter notre site Web pour avoir la dernière mise à jour. ** The number of places available may vary. Please check our Web site to find out the current availability. Si vous désirez ...

  6. A Novel Cell Type Enables B. subtilis to Escape from Unsuccessful Sporulation in Minimal Medium.

    Science.gov (United States)

    Defeu Soufo, Hervé Joël

    2016-01-01

    Sporulation is the most enduring survival strategy developed by several bacterial species. However, spore development of the model organism Bacillus subtilis has mainly been studied by means of media or conditions optimized for the induction of sporogenesis. Here, I show that during prolonged growth during stationary phase in minimal medium, B. subtilis undergoes an asymmetric cell division that produces small and round-shaped, DNA containing cells. In contrast to wild-type cells, mutants harboring spo0A or spoIIIE / sftA double mutations neither sporulate nor produce this special cell type, providing evidence that the small round cells emerge from the abortion of endospore formation. In most cases observed, the small round cells arise in the presence of sigma H but absence of sigma F activity, different from cases of abortive sporulation described for rich media. These data suggest that in minimal media, many cells are able to initiate but fail to complete spore development, and therefore return to normal growth as rods. This work reveals that the continuation of asymmetric cell division, which results in the formation of the small round cells, is a way for cells to delay or escape from-unsuccessful-sporulation. Based on these findings, I suggest to name the here described cell type as "dwarf cells" to distinguish them from the well-known minicells observed in mutants defective in septum placement or proper chromosome partitioning.

  7. Virulence, sporulation, and elicitin production in three clonal lineages of Phytophthora ramorum

    Science.gov (United States)

    Phytophthora ramorum populations are clonal and consist of three lineages. Recent studies have shown that the clonal lineages may have varying degrees of aggressiveness on some host species, such as Quercus rubra. In this study, we examined virulence, sporulation and elicitin production of five P. ...

  8. A Novel Cell Type Enables B. subtilis To Escape From Unsuccessful Sporulation In Minimal Medium

    Directory of Open Access Journals (Sweden)

    Herve Joel Defeu Soufo

    2016-11-01

    Full Text Available Sporulation is the most enduring survival strategy developed by several bacterial species. However, spore development of the model organism Bacillus subtilis has mainly been studied by means of media or conditions optimized for the induction of sporogenesis. Here, I show that during prolonged growth during stationary phase in minimal medium, B. subtilis undergoes an asymmetric cell division that produces small and round-shaped, DNA containing cells. In contrast to wild-type cells, mutants harboring spo0A or spoIIIE/sftA double mutations neither sporulate nor produce this special cell type, providing evidence that the small round cells emerge from the abortion of endospore formation. In most cases observed, the small round cells arise in the presence of sigma H but absence of sigma F activity, different from cases of abortive sporulation described for rich media. These data suggest that in minimal media, many cells are able to initiate but fail to complete spore development, and therefore return to normal growth as rods. This work reveals that the continuation of asymmetric cell division, which results in the formation of the small round cells, is a way for cells to delay or escape from - unsuccessful - sporulation. Based on these findings, I suggest to name the here described cell type as dwarf cells to distinguish them from the well-known minicells observed in mutants defective in septum placement or proper chromosome partitioning.

  9. Sporulation and Germination patterns - hedging a bet on long term microbial survivability in dry soil

    Science.gov (United States)

    Claes, N.; Or, D.

    2012-04-01

    Soil hosts unparalleled diversity of microbial life that is constantly challenged by the vagaries of fluctuating ambient conditions. Desiccation stresses play a key role not only by directly affecting individual bacterial cells, but also by shaping diffusion pathways and cell dispersion. The gradual thinning and fragmentation of the aqueous environment during drying have led to different survival mechanisms including dormancy and sporulation, resulting in a highly resistive state capable of surviving extreme and prolonged environmental stresses until conditions improve in the future. Our aim is to investigate how temporal changes in hydration status shape microbial communities over time, based on simple survival strategy rules for each individual bacterium. The two survival strategies considered are dormancy and sporulation. Dormancy is the state in which bacterial cells significantly reduce their metabolism with minor morphological adaptations. The required energy and time for attaining this state are low relative to sporulation costs. Sporulation involves several morphological and biochemical changes that result in a resistive capsule that endures extreme stresses over long periods of time. The working hypothesis is that different micro-ecological conditions and community compositions would result from temporal patterns and magnitude of desiccation stresses. An Individual Based Model (IBM) considering habitats on rough soil surfaces and local effects of micro-hydrological conditions on dispersion and nutrient diffusion would enable systematic study of emerging communities over extended periods. Different population compositions are expected to emerge based on low and high frequency, duration and amplitudes of wetting-drying cycles reflecting relative success or failure of survival strategy.

  10. Sporulation environment influences spore properties in Bacillus: evidence and insights on underlying molecular and physiological mechanisms.

    Science.gov (United States)

    Bressuire-Isoard, Christelle; Broussolle, Véronique; Carlin, Frédéric

    2018-05-17

    Bacterial spores are resistant to physical and chemical insults, which make them a major concern for public health and for industry. Spores help bacteria to survive extreme environmental conditions that vegetative cells cannot tolerate. Spore resistance and dormancy are important properties for applications in medicine, veterinary health, food safety, crop protection, and other domains. The resistance of bacterial spores results from a protective multilayered structure and from the unique composition of the spore core. The mechanisms of sporulation and germination, the first stage after breaking of dormancy, and organization of spore structure have been extensively studied in Bacillus species. This review aims to illustrate how far the structure, composition and properties of spores are shaped by the environmental conditions in which spores form. We look at the physiological and molecular mechanisms underpinning how sporulation media and environment deeply affect spore yield, spore properties like resistance to wet heat and physical and chemical agents, germination, and further growth. For example, spore core water content decreases as sporulation temperature increases, and resistance to wet heat increases. Controlling the fate of Bacillus spores is pivotal to controlling bacterial risks and process efficiencies in, for example, the food industry, and better control hinges on better understanding how sporulation conditions influence spore properties.

  11. Prediction of quantitative phenotypes based on genetic networks: a case study in yeast sporulation

    Directory of Open Access Journals (Sweden)

    Shen Li

    2010-09-01

    Full Text Available Abstract Background An exciting application of genetic network is to predict phenotypic consequences for environmental cues or genetic perturbations. However, de novo prediction for quantitative phenotypes based on network topology is always a challenging task. Results Using yeast sporulation as a model system, we have assembled a genetic network from literature and exploited Boolean network to predict sporulation efficiency change upon deleting individual genes. We observe that predictions based on the curated network correlate well with the experimentally measured values. In addition, computational analysis reveals the robustness and hysteresis of the yeast sporulation network and uncovers several patterns of sporulation efficiency change caused by double gene deletion. These discoveries may guide future investigation of underlying mechanisms. We have also shown that a hybridized genetic network reconstructed from both temporal microarray data and literature is able to achieve a satisfactory prediction accuracy of the same quantitative phenotypes. Conclusions This case study illustrates the value of predicting quantitative phenotypes based on genetic network and provides a generic approach.

  12. Differences in virulence and sporulation of Phytophthora kernoviae isolates originating from two distinct geographical regions

    Science.gov (United States)

    Phytophthora kernoviae has only been isolated from the United Kingdom (U.K.) and New Zealand. To understand what differences may exist between isolates from these two distinct geographical regions, virulence studies on three host plants and sporulation on host leaves were conducted on select isolat...

  13. The identification of four histidine kinases that influence sporulation in Clostridium thermocellum.

    Science.gov (United States)

    Mearls, Elizabeth B; Lynd, Lee R

    2014-08-01

    In this study, we sought to identify genes involved in the onset of spore formation in Clostridium thermocellum via targeted gene deletions, gene over-expression, and transcriptional analysis. We determined that three putative histidine kinases, clo1313_0286, clo1313_2735 and clo1313_1942 were positive regulators of sporulation, while a fourth kinase, clo1313_1973, acted as a negative regulator. Unlike Bacillus or other Clostridium species, the deletion of a single positively regulating kinase was sufficient to abolish sporulation in this organism. Sporulation could be restored in these asporogenous strains via overexpression of any one of the positive regulators, indicating a high level of redundancy between these kinases. In addition to having a sporulation defect, deletion of clo1313_2735 produced L-forms. Thus, this kinase may play an additional role in repressing L-form formation. This work suggests that C. thermocellum enters non-growth states based on the sensory input from multiple histidine kinases. The ability to control the development of non-growth states at the genetic level has the potential to inform strategies for improved strain development, as well as provide valuable insight into C. thermocellum biology. Copyright © 2014 Elsevier Ltd. All rights reserved.

  14. Microarray analysis identified Puccinia striiformis f. sp. tritici genes involved in infection and sporulation.

    Science.gov (United States)

    Puccinia striiformis f. sp. tritici (Pst) causes stripe rust, one of the most important diseases of wheat worldwide. To identify Pst genes involved in infection and sporulation, a custom oligonucleotide Genechip was made using sequences of 442 genes selected from Pst cDNA libraries. Microarray analy...

  15. Regulation of cellulase expression, sporulation, and morphogenesis by velvet family proteins in Trichoderma reesei.

    Science.gov (United States)

    Liu, Kuimei; Dong, Yanmei; Wang, Fangzhong; Jiang, Baojie; Wang, Mingyu; Fang, Xu

    2016-01-01

    Homologs of the velvet protein family are encoded by the ve1, vel2, and vel3 genes in Trichoderma reesei. To test their regulatory functions, the velvet protein-coding genes were disrupted, generating Δve1, Δvel2, and Δvel3 strains. The phenotypic features of these strains were examined to identify their functions in morphogenesis, sporulation, and cellulase expression. The three velvet-deficient strains produced more hyphal branches, indicating that velvet family proteins participate in the morphogenesis in T. reesei. Deletion of ve1 and vel3 did not affect biomass accumulation, while deletion of vel2 led to a significantly hampered growth when cellulose was used as the sole carbon source in the medium. The deletion of either ve1 or vel2 led to the sharp decrease of sporulation as well as a global downregulation of cellulase-coding genes. In contrast, although the expression of cellulase-coding genes of the ∆vel3 strain was downregulated in the dark, their expression in light condition was unaffected. Sporulation was hampered in the ∆vel3 strain. These results suggest that Ve1 and Vel2 play major roles, whereas Vel3 plays a minor role in sporulation, morphogenesis, and cellulase expression.

  16. Molecular Mechanisms Regulating Sporulation in the Filamentous Fungus Ashbya gossypii

    DEFF Research Database (Denmark)

    Wasserstrom, Lisa

    Sporulation is a complex developmental program that fungi enter to ensure survival in unfavorable environmental conditions. Many fungal species are able to produce spores sexually through meiosis, which is beneficial since it introduces genetic variability into a population. The sexually reproduc...

  17. Influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina

    Directory of Open Access Journals (Sweden)

    Diana Erica Gómez

    2013-06-01

    Full Text Available Fungi require special substrates for their isolation, vegetative growth and sporulation. In experiments conducted in the laboratory, the influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina conidia, the causal agent of soybean frogeye leaf spot, was assessed. The media potato sucrose agar, V-8 agar, tomato extract agar, soybean leaf extract agar, soybean seed extract agar, soybean meal agar, soybean flour agar and wheat flour agar were tested, added on the surface, with and without filter paper and under two light regimes, with 12 h light at 25°± 2°C and in the dark. A triple factorial 8x2x2 (substrates x light/dark x with/without filter paper design with four replicates was used. V-8 agar medium was employed and the pH was adjusted with HCl 0.1N or NaOH 0.1N before autoclaving to the values: 3, 4, 5, 6, 7 and 8, and the pH of V-8 agar medium is 6.7. The evaluation was done on the seventh day of incubation. Data underwent regression analysis. Sporulation was maximized on the agar media V-8, seed extract, oat flour, tomato extract, and potato sucrose in the presence of filter paper and 12h light. On V-8 medium, maximal sporulation was obtained with pH 6.7.

  18. Taking Stock.

    Science.gov (United States)

    Merriam, Sharan B.

    1993-01-01

    A complete theory of adult learning must take into consideration the learner, learning process, and context. Andragogy, self-directed learning, consciousness, critical theory, feminism, transformational learning, and situated cognition contribute to understanding of this complex phenomenon. (SK)

  19. Planned place of birth

    DEFF Research Database (Denmark)

    Overgaard, Charlotte; Coxon, Kirstie; Stewart, Mary

    Title Planned place of birth: issues of choice, access and equity. Outline In Northern European countries, giving birth is generally safe for healthy women with uncomplicated pregnancies, and their babies. However, place of birth can affect women’s outcomes and experiences of birth. Whilst tertiary...... countries, maternity care is provided free to women, through public financing of health care; universal access to care is therefore secured. Nevertheless, different models of care exist, and debates about the appropriateness of providing maternity care in different settings take place in both countries...... in Denmark Coxon K et al: Planned place of birth in England: perceptions of accessing obstetric units, midwife led units and home birth amongst women and their partners. How these papers interrelate These papers draw upon recent research in maternity care, undertaken in Denmark and in England. In both...

  20. Places available**

    CERN Multimedia

    Places are available in the following courses: Hands-on Introduction to Python Programming: 11-13.08.2003 (3 days) Introduction to the CERN Engineering Data Management System (EDMS): 26.08.2003 (1 day) The CERN Engineering Data Management System (EDMS) for Engineers: 27.08.2003 (1 day) CLEAN-2002 : Travailler en salle blanche : 4.09.2003 (une demi-journée) AutoCAD 2002 - Level 1: 4, 5, 15, 16.09.2003 (2 x 2 days) AutoCAD Mechanical 6 PowerPack : 17, 18, 25, 26.09.2003 et 2, 3.10.2003 (3 x 2 journées, français) AutoCAD 2002 - niveau 1 : 23, 24, 30.09.2003 et 1.10.2003 (2 x 2 journées) Introduction to the CERN Engineering Data Management System (EDMS): 23.09.2003 (1 day) The CERN Engineering Data Management System (EDMS) for Local Administrators: 24-25.09.2003 (2 days) AutoCAD 2002 - niveau 2 : 8 et 10.10.2003 (2 journées) CLEAN-2002: Working in a Cleanroom: 23.10.2003 (half day, p.m.) ** The number of places available may vary. Please check our Web site to find out the current availabili...

  1. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: Hands-on Introduction to Python Programming: 11-13.08.2003(3 days) Introduction to the CERN Engineering Data Management System (EDMS): 26.08.2003 (1 day) The CERN Engineering Data Management System (EDMS) for Engineers: 27.08.2003 (1 day) CLEAN-2002 : Travailler en salle blanche : 4.09.2003 (une demi-journée) AutoCAD 2002 - Level 1: 4, 5, 15, 16.09.2003 (2 x 2 days) AutoCAD Mechanical 6 PowerPack : 17, 18, 25, 26.09.2003 et 2, 3.10.2003 (3 x 2 journées, français) AutoCAD 2002 - niveau 1 : 23, 24, 30.09.2003 et 1.10.2003 (2 x 2 journées) Introduction to the CERN Engineering Data Management System (EDMS): 23.09.2003 (1 day) The CERN Engineering Data Management System (EDMS) for Local Administrators: 24-25.09.2003 (2 days) AutoCAD 2002 - niveau 2 : 8 et 10.10.2003 (2 journées) CLEAN-2002: Working in a Cleanroom: 23.10.2003 (half day, p.m.) ** The number of places available may vary. Please ch...

  2. Media places

    DEFF Research Database (Denmark)

    Linde, Per; Messeter, Jörn

    The impact that ubiquitous wireless network technologies and mobile phones have on our experience of the modern cityscape, has been a driving force in many research projects in recent years. The agendas differ in relation to perspectives, but it seems safe to claim that such technologies are no l......The impact that ubiquitous wireless network technologies and mobile phones have on our experience of the modern cityscape, has been a driving force in many research projects in recent years. The agendas differ in relation to perspectives, but it seems safe to claim that such technologies...... construction of place in the urban setting. The concept of Hertzian space, put forth by Anthony Dunne and others (Dunne, 1999) also carries a dimension of how spaces of wireless communication may be problematized, and how we can criticize cultural phenomena taken for granted through innovative technology. From...... this perspective wireless technology can also be a way of temporarily appropriating places within the city space for a variety of different groups, at times questioning hierarchical structures of ownership of public spaces. These spaces can be said to be hybrid spaces, bringing forth the fundamental question...

  3. Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval Tel. 74924technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: MATLAB Fundamentals and Programming Techniques (ML01) : 2 & 3.12.03 (2 days) Oracle 8i : SQL : 3 - 5.12.03 (3 days) The EDMS MTF in practice : 5.12.03 (afternoon, free of charge) Modeling Dynamic Systems with Simulink (SL01) : 8 & 9.12.03 (2 days) Signal Processing with MATLAB (SG01) : 11 & 12.12.03 (2 days) The JAVA Programming Language - l...

  4. Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: MATLAB Fundamentals and Programming Techniques (ML01) :2 & 3.12.03 (2 days) Oracle 8i : SQL : 3 - 5.12.03 (3 days) The EDMS MTF in practice : 5.12.03 (afternoon, free of charge) Modeling Dynamic Systems with Simulink (SL01) : 8 & 9.12.03 (2 days) Signal Processing with MATLAB (SG01) : 11 & ...

  5. Places available**

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The JAVA Programming Language Level 1 : 9 & 10.1.2004 (2 days) The JAVA Programming Language Level 2 : 11 to 13.1.2004 (3 days) LabVIEW base 1 : 25 - 27.2.2004 (3 jours) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon - free of charge) C++ for Particle Physicists : 8 - 12.3.2004 ( 6 X 4-hour sessions) LabVIEW Basics 1 : 22 - 24.3.20...

  6. Places available**

    CERN Document Server

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The JAVA Programming Language Level 1 :9 & 10.1.2004 (2 days) The JAVA Programming Language Level 2 : 11 to 13.1.2004 (3 days) Hands-on Introduction to Python Programming : 16 - 18.2.2004 (3 days - free of charge) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon - free of charge) C++ for Particle Physicists : 8 - 12.3.2004...

  7. Places available**

    CERN Document Server

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval Tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: JAVA 2 Enterprise Edition - Part 1 : WEB Applications : 20 & 21.11.03(2 days) FrontPage 2000 - niveau 1 : 20 & 21.11.03 (2 jours) Oracle 8i : SQL : 3 - 5.12.03 (3 days) Oracle 8i : Programming with PL/SQL : 8 - 10.12.03 (3 days) The JAVA Programming Language - leve...

  8. Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Project Planning with MS-Project : 15 & 22.1.2004 (2 days) Joint PVSS JCOP Framework Course : 2 sessions : 2 - 6.2.2004 and 16 - 20-2-2004 (5 days) Hands-on Introduction to Python Programming : 16 - 18.2.2004 (3 days - free of charge) C++ for Particle Physicists : 8 - 12.3.2004 ( 6 X 4-hour sessions)

  9. Places available **

    CERN Multimedia

    2003-01-01

    Des places sont disponibles dans les cours suivants : Places are available in the following courses : WorldFIP 2003 pour utilisateurs : 11-14.2.03 (4 jours) DISP-2003 ? Spring I Term : Introduction to Digital Signal Processing : 20, 27.2, 6, 13, 20, 27.3, 3.4.03 (7 X 2-hour lectures) AXEL-2003 - Introduction to Accelerators : 24-28.2.03 (10 X 1-hour lectures) AutoCAD 2002 - niveau 1 : 24, 25.2 & 3, 4.3.03 (4 jours) Introduction à Windows 2000 au CERN : 25.2.03 (1/2 journée) LabView base 2/LabView Basics 2 : 10 & 11.3.03 (2 jours/2 days) langue à définir/Language to be decided C++ for Particle Physicists : 10 ? 14.3.03 (6 X 3-hour lectures) Introduction to PVSS : 10.3.03 (half day, afternoon) Basic PVSS : 11 - 13.3.03 (3 days) LabView avancé /LabView Advanced : 12 - 14.3.03 (3 jours/3days) Langue à définir/language to be decided AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 17, 18, 24 & 25.3.03 (6 jours) PVSS - JCOP Framework Tutorial : 14.3.03 (1 day) MAGNE-03 - Magnetism for Technical Ele...

  10. Places available **

    CERN Multimedia

    2003-01-01

    Des places sont disponibles dans les cours suivants : Places are available in the following courses : Introduction à Windows 2000 au CERN : 25.2.03 (1/2 journée) LabView base 2/LabView Basics 2 : 10 & 11.3.03 (2 jours/2 days) langue à définir/Language to be decided C++ for Particle Physicists : 10 - 14.3.03 (6 X 3-hour lectures) Introduction to PVSS : 10.3.03 (half day, afternoon) Basic PVSS : 11 - 13.3.03 (3 days) LabView avancé /LabView Advanced : 12 - 14.3.03 (3 jours/3days) Langue à définir/Language to be decided AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 17, 18, 24 & 25.3.03 (6 jours) PVSS - JCOP Framework Tutorial : 14.3.03 (1 day) CLEAN-2002 : Working in a cleanroom : 2.4.03 (half-day, afternoon, free course, registration required) LabView base 1/LabView Basics 1 : 9 - 11.4.03 (3 jours/3 days) Langue à définir/Language to be decided DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (...

  11. Places available **

    CERN Multimedia

    2003-01-01

    Des places sont disponibles dans les cours suivants : Places are available in the following courses : DISP-2003 - Spring I Term : Introduction to Digital Signal Processing : 20, 27.2, 6, 13, 20, 27.3, 3.4.03 (7 X 2-hour lectures) AXEL-2003 - Introduction to Accelerators : 24 - 28.2.03 (10 X 1-hour lectures) AutoCAD 2002 - niveau 1 : 24, 25.2 & 3, 4.3.03 (4 jours) Introduction à Windows 2000 au CERN : 25.2.03 (1/2 journée) LabView base 2/LabView Basics 2 : 10 & 11.3.03 (2 jours/2 days) langue à définir/Language to be decided C++ for Particle Physicists : 10 - 14.3.03 (6 X 3-hour lectures) Introduction to PVSS : 10.3.03 (half day, afternoon) Basic PVSS : 11 - 13.3.03 (3 days) LabView avancé /LabView Advanced : 12 - 14.3.03 (3 jours/3days) Langue à définir/language to be decided AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 17, 18, 24 & 25.3.03 (6 jours) PVSS - JCOP Framework Tutorial : 14.3.03 (1 day) CLEAN-2002 : Working in a cleanroom : 2.4.03 (half-day, afternoon, free course, regis...

  12. Places available **

    CERN Multimedia

    2003-01-01

    Des places sont disponibles dans les cours suivants : Places are available in the following courses : C++ for Particle Physicists : 10 - 14.3.03 (6 X 3-hour lectures) Introduction to PVSS : 10.3.03 (half day, afternoon) Basic PVSS : 11 - 13.3.03 (3 days) PVSS - JCOP Framework Tutorial : 14.3.03 (1 day) CLEAN-2002 : Working in a cleanroom : 2.4.03 (half-day, afternoon, free course, registration required) LabView base 1/LabView Basics 1 : 9 - 11.4.03 (3 jours/3 days) Langue à définir/language to be decided DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03 (6 jours) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 11 & 12.3.03 / 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 31.3 - 4.4.03 / 16...

  13. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: Conception de PCB rapides dans le flot Cadence : 11.6.03 (matin) EXCEL 2000 - level 1 : 12 & 13.6.03 (2 days) Introduction to PVSS : 16.6.03 (p.m.) Basic PVSS : 17 - 19.6.03 (3 days) Réalisation de PCB rapides dans le flot Cadence : 17.6.03 (matin) PVSS - JCOP Framework Tutorial : 20.6.03 (1 day) Programmation automate Schneider : Programmation automate Schneider TSX Premium - 2ème niveau : 24 - 27.6.03 (4 jours) - audience : toute personne qui veux maitriser la mise en uvre et la programmation des fonctions spécialisées d'un automate TSX Premium - objectifs : maitriser la mise en uvre et la programmation des fonctions spécialisées d'un automate TSX Premium Cours de sécurité : Etre TSO au CERN : Prochaines sessions : 24, 25 & 27.6.03 - 4, 5 & 7.11.03 (session de 3 jours) ** The number of places available may vary. Please check our Web site to find out the current availability. If you wish to participate in one of these courses, pl...

  14. Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses : EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) CLEAN-2002 : Working in a Cleanroom (free of charge) : 23.10.03 (half day) The EDMS-MTF in practice (free of charge) :  28 -  30.10.03 (6 half-day sessions) AutoCAD 2002 - Level 1 : 3, 4, 12, 13.11.03 (4 days) LabVIEW TestStand ver. 3 : 4 & 5.11.03 (2 days) Introduction to Pspice : 4.11.03 p.m. (half-day) Hands-on Introduction to Python Programm...

  15. PLACES AVAILABLE

    CERN Multimedia

    Enseignement Technique; Tél. 74924; Technical Training; Monique Duval; Tel. 74924

    2000-01-01

    Places available Places are available in the following courses:   LabView hands-on 13.11.00 4 hours LabView Basics 1 14 - 16.11.00 3 days Nouveautés de WORD 19 et 20.10.00 2 jours ACCESS 1er niveau 30 - 31.10.00 2 jours Advanced aspects of the C language 2 - 3.11.00 2 days Introduction to Oracle SQL and PL/SQL 13 - 17.11.00 5 days C++ for Particle Physicists 20 - 24.11.00 6 lectures Develop PL/SQL Program Units 20 - 22.11.00 3 days Oracle Application Server Develop Web-Based Applications with PL/SQL 27 - 28.11.00 2 days Programmation TSX Premium 1 28.11 - 1.12.00 4 jours Programmation TSX Premium 2 12 - 15.12.00 4 jours If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an “application for training” form available from your Divisional Secretariat or from your DTO (Divisional Training Offi...

  16. High-Throughput Genetic Screens Identify a Large and Diverse Collection of New Sporulation Genes in Bacillus subtilis.

    Science.gov (United States)

    Meeske, Alexander J; Rodrigues, Christopher D A; Brady, Jacqueline; Lim, Hoong Chuin; Bernhardt, Thomas G; Rudner, David Z

    2016-01-01

    The differentiation of the bacterium Bacillus subtilis into a dormant spore is among the most well-characterized developmental pathways in biology. Classical genetic screens performed over the past half century identified scores of factors involved in every step of this morphological process. More recently, transcriptional profiling uncovered additional sporulation-induced genes required for successful spore development. Here, we used transposon-sequencing (Tn-seq) to assess whether there were any sporulation genes left to be discovered. Our screen identified 133 out of the 148 genes with known sporulation defects. Surprisingly, we discovered 24 additional genes that had not been previously implicated in spore formation. To investigate their functions, we used fluorescence microscopy to survey early, middle, and late stages of differentiation of null mutants from the B. subtilis ordered knockout collection. This analysis identified mutants that are delayed in the initiation of sporulation, defective in membrane remodeling, and impaired in spore maturation. Several mutants had novel sporulation phenotypes. We performed in-depth characterization of two new factors that participate in cell-cell signaling pathways during sporulation. One (SpoIIT) functions in the activation of σE in the mother cell; the other (SpoIIIL) is required for σG activity in the forespore. Our analysis also revealed that as many as 36 sporulation-induced genes with no previously reported mutant phenotypes are required for timely spore maturation. Finally, we discovered a large set of transposon insertions that trigger premature initiation of sporulation. Our results highlight the power of Tn-seq for the discovery of new genes and novel pathways in sporulation and, combined with the recently completed null mutant collection, open the door for similar screens in other, less well-characterized processes.

  17. High-Throughput Genetic Screens Identify a Large and Diverse Collection of New Sporulation Genes in Bacillus subtilis

    Science.gov (United States)

    Brady, Jacqueline; Lim, Hoong Chuin; Bernhardt, Thomas G.; Rudner, David Z.

    2016-01-01

    The differentiation of the bacterium Bacillus subtilis into a dormant spore is among the most well-characterized developmental pathways in biology. Classical genetic screens performed over the past half century identified scores of factors involved in every step of this morphological process. More recently, transcriptional profiling uncovered additional sporulation-induced genes required for successful spore development. Here, we used transposon-sequencing (Tn-seq) to assess whether there were any sporulation genes left to be discovered. Our screen identified 133 out of the 148 genes with known sporulation defects. Surprisingly, we discovered 24 additional genes that had not been previously implicated in spore formation. To investigate their functions, we used fluorescence microscopy to survey early, middle, and late stages of differentiation of null mutants from the B. subtilis ordered knockout collection. This analysis identified mutants that are delayed in the initiation of sporulation, defective in membrane remodeling, and impaired in spore maturation. Several mutants had novel sporulation phenotypes. We performed in-depth characterization of two new factors that participate in cell–cell signaling pathways during sporulation. One (SpoIIT) functions in the activation of σE in the mother cell; the other (SpoIIIL) is required for σG activity in the forespore. Our analysis also revealed that as many as 36 sporulation-induced genes with no previously reported mutant phenotypes are required for timely spore maturation. Finally, we discovered a large set of transposon insertions that trigger premature initiation of sporulation. Our results highlight the power of Tn-seq for the discovery of new genes and novel pathways in sporulation and, combined with the recently completed null mutant collection, open the door for similar screens in other, less well-characterized processes. PMID:26735940

  18. A Mutation in the Bacillus subtilis rsbU Gene That Limits RNA Synthesis during Sporulation.

    Science.gov (United States)

    Rothstein, David M; Lazinski, David; Osburne, Marcia S; Sonenshein, Abraham L

    2017-07-15

    Mutants of Bacillis subtilis that are temperature sensitive for RNA synthesis during sporulation were isolated after selection with a 32 P suicide agent. Whole-genome sequencing revealed that two of the mutants carried an identical lesion in the rsbU gene, which encodes a phosphatase that indirectly activates SigB, the stress-responsive RNA polymerase sigma factor. The mutation appeared to cause RsbU to be hyperactive, because the mutants were more resistant than the parent strain to ethanol stress. In support of this hypothesis, pseudorevertants that regained wild-type levels of sporulation at high temperature had secondary mutations that prevented expression of the mutant rsbU gene. The properties of these RsbU mutants support the idea that activation of SigB diminishes the bacterium's ability to sporulate. IMPORTANCE Most bacterial species encode multiple RNA polymerase promoter recognition subunits (sigma factors). Each sigma factor directs RNA polymerase to different sets of genes; each gene set typically encodes proteins important for responses to specific environmental conditions, such as changes in temperature, salt concentration, and nutrient availability. A selection for mutants of Bacillus subtilis that are temperature sensitive for RNA synthesis during sporulation unexpectedly yielded strains with a point mutation in rsbU , a gene that encodes a protein that normally activates sigma factor B (SigB) under conditions of salt stress. The mutation appears to cause RsbU, and therefore SigB, to be active inappropriately, thereby inhibiting, directly or indirectly, the ability of the cells to transcribe sporulation genes. Copyright © 2017 American Society for Microbiology.

  19. Place identity and place scale: the impact of place salience.

    OpenAIRE

    Bernardo, Fátima; Palma-Oliveira, José-Manuel

    2013-01-01

    Research about place, place identity and attachment supports the idea that bonds with places may differ depending on the place scale. Based on the view that identity is context-dependent, this paper brings to the table the impact of manipulating the salience of place on the intensity of place identity and place attachment reported. A study was designed to examine place identity and place attachment in two groups of residents (permanent and temporary) at three different scales (nei...

  20. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Introduction to Databases :  3 - 4.7.01 (2 days) The JAVA programming language Level 2 : 4 - 6.7.01 (3 days) Enterprise JavaBeans :  9 - 11.7.01 (3 days) Design Patterns :  10 - 12.7.01 (3 days) C++ for Particle Physicists :  23 - 27.7.01 (6 3-hour lectures) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

  1. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Introduction to Perl 5 : 2 - 3.7.01 (2 days) Introduction to Databases :  3 - 4.7.01 (2 days) JAVA programming language Level 2 : 4 - 6.7.01 (3 days) Enterprise JavaBeans :  9 - 11.7.01 (3 days) Design Patterns :  10 - 12.7.01 (3 days) C++ for Particle Physicists :  23 - 27.7.01 (6 3-hour lectures) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

  2. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: CLEAN-2002 : Travailler en salle blanche (cours gratuit) : 13.08.2002 (matin) Introduction to the CERN Enginnering Data Management System :  27.8.02  (1 day) The CERN Engineering Data Management System for Advanced Users :  28.8.02  (1 day) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval Tel.74924 monique.duval@cern.ch    

  3. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: C++ Programming Level 2 - Traps & Pitfalls:  16 - 19.7.02 (4 days) Frontpage 2000 - level 1 :  22 - 23.7.02  (2 days) Introduction à Windows 2000 au CERN : 24.7.02 (après-midi) CLEAN-2002 : Travailler en salle blanche (cours gratuit) : 13.08.2002 (matin) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval Tel.74924 monique.duval@cern.ch

  4. Placing knowledge

    DEFF Research Database (Denmark)

    Adriansen, Hanne Kirstine; Valentin, Karen; Nielsen, Gritt B.

    ; on the other hand, the rationale for strengthening mobility through internationalisation is based on an imagination of the potentials of particular locations (academic institutions). Intrigued by this tension between universality and particularity in academic knowledge production, this paper presents...... preliminary findings from a project that study internationalisation of higher education as an agent in the interrelated processes of place-making and knowledge-making. The project is based on three case-studies. In this paper, focus is on PhD students’ change of research environment. This is used as a case......Internationalisation of higher education is premised by a seeming paradox: On the one hand, academic knowledge strives to be universal in the sense that it claims to produce generalizable, valid and reliable knowledge that can be used, critiqued, and redeveloped by academics from all over the world...

  5. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: December 2002   PCAD Schémas - Débutants :  5 & 6.12.02  (2 jours) PCAD PCB - Débutants :  9 - 11.12.02  (3 jours) FrontPage 2000 - level 1:  9 & 10.12.02  (2 days) Introduction à la CAO Cadence (cours gratuit) :  10 & 11.12.02  (2 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval Tel.74924 monique.duval@cern.ch

  6. Places available **

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: PIPES-2003 - Pratique du Sertissage de tubes métalliques et multicouches : 26.8.03 (stage pratique) The CERN Engineering Data Management System (EDMS) for Engineers : 27.8.03 (1 day, free of charge) CLEAN-2002 : Travailler en salle blanche : 4.9.03 (une demi-journée, séminaire gratuit) The CERN Engineering Data Management System (EDMS) for Local Administrators : 24 & 25.9.03 (2 days, free of charge) Siemens SIMATIC Training : Programmation STEP7 - niveau 1 : 29 - 2.10.03 (4 jours) - ouverture des inscriptions fin août Programmation STEP7 - niveau 2 : 13 - 17.10.03 (5 jours) - ouverture des inscriptions fin août Réseau Simatic Net : 22 & 23.10.03 (2 jours) - ouverture des inscriptions fin août CLEAN-2002 : Working in a Cleanroom : 23.20.03 (half day, free of charge) These courses will be given in French or Englis...

  7. Places available**

    CERN Document Server

    2003-01-01

    Places are available in the following courses : FrontPage 2000 - niveau 1: 20 & 21.5.03 (2 jours) PIPES-2003 : Pratique du sertissage de tubes métalliques et multicouches: 21.5.03 (1 jour) Introduction à la CAO Cadence: de la saisie de schéma Concept-HDL au PCB : 20 & 22.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (E): 5, 6, 12, 13, 26, 27.6.03 (6 days) EXCEL 2000 - niveau 1: 10 & 11.6.03 (2 jours) Conception de PCB rapides dans le flot Cadence: 11.6.03 (matin) EXCEL 2000 - level 1: 12 & 13.6.03 (2 days) Introduction to PVSS: 16.6.03 (half-day, pm) Basic PVSS: 17 - 19.6.03 (3 days) Réalisation de PCB rapides dans le flot Cadence: 17.6.03 (matin) LabView DSC (language to be defined): 19 & 20.6.03 PVSS - JCOP Framework Tutorial: 20.6.03 (1 day) EXCEL 2000 - niveau 2: 24 & 25.6.03 (2 jours) Siemens SIMATIC Training: Introduction to STEP7: 3 & 4.6.03 (2 days) STEP7 Programming: 16 - 20.6.03 (5 days) Simatic Net Network: 26 & 27.6.03 (2 days) These courses will be given...

  8. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: LabView Hands-on (bilingue/bilingual - gratuit/free of charge) : 13.9.02 (a.m.) LabView DAQ Hands-on (bilingue/bilingual - gratuit/free of charge) : 13.9.02 (p.m.) AutoCAD 2002 - niveau 1 : 19, 20, 26, 27.9.02 (4 jours) LabView Base 1 : 23 - 25.9.02 (3 jours) LabView DAQ (E) : 26 - 27.9.02 (2 days) AutoCAD Mechanical 6 PowerPack (F) : 30.9, 1, 2, 9, 10, 11.10.02 (6 jours) CLEAN-2002 : Working in a Cleanroom (free of charge) : 10.10.02 (half-day, p.m.) AutoCAD 2002 - niveau 2 : 14 - 15.10.02 (2 jours) AutoCAD 2002 - Level 1 : 17, 18, 24, 25.10.02 (4 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Of...

  9. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Introduction to Oracle 8i : SQL and PL/SQL:  7 - 11.10.02  (5 days) CLEAN-2002 : Working in a Cleanroom (free of charge):  10.10.02  (half-day, p.m.) AutoCAD 2002 - niveau 2 :  14 - 15.10.02  (2 jours) Introduction à DesignSpace :  16.10.02  (1 journée) Introduction to DesignSpace:  17.10.02  (1 day) AutoCAD 2002 - Level 1:  17, 18, 24, 25.10.02  (4 days) AutoCAD Mechanical 6 PowerPack (F) :  21, 22, 23.10 et 4, 5, 6.11.02  (6 jours) Introduction à ANSYS/Introduction to ANSYS (langue à définir suivant demande/ Language to be chosen according to demand):  21 - 25.10.02  (5 jours/days) HREF-2002: Helium Refrigeration Techniques (English-French, bilingual) :  21 - 25.10.2002  (7 half days) HREF-2002: Techniques de la Réfri...

  10. Places available**

    CERN Document Server

    2003-01-01

    Places are available in the following courses : The CERN EDMS for Local Administrators : 24 & 25.9.03 (2 days, free of charge) HeREF-2003 : Techniques de la réfrigération Hélium (cours en français avec support en anglais) : 6 - 10.10.2003 (7 demi-journées) The Java Programming Language Level 1 : 6 - 7.10.2003 (2 days) Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) AutoCAD 2002 - niveau 1 : 20, 21, 27, 28.10.03 (4 jours) CLEAN-2002 : Working in a Cleanroom : 23.10.03 (half day, free of charge) AutoCAD 2002 - Level 1 : 3, 4, 12, 13.11.03 (4 days) AutoCAD 2002 - niveau 2 : 10 & 11.11.03 (2 jours) ACCESS 2000 - niveau 1 : 13 & 14.11.03 (2 jours) AutoCAD Mechanical 6 PowerPack (E) : 17, 18, 24, 25.11 & 1, 2.12.03 (6...

  11. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Introduction à DesignSpace :  16.10.02  (1 journée) AutoCAD Mechanical 6 PowerPack (F) :  21, 22, 23.10 et 4, 5, 6.11.02  (6 jours) Introduction à ANSYS 21 - 25.10.02  (5 jours/days) HREF-2002: Helium Refrigeration Techniques (English-French, bilingual) :  21 - 25.10.2002  (7 half days) LabVIEW Basics 1 (English):  21 - 23.10.02  (3 days) LabVIEW Basics 2 (English):  24 & 25.10.02  (2 days) Oracle 8i : Access the Database with Java:  7 & 8.11.02  (2 days) AutoCAD 2002 - niveau 2 :  7 & 8.11.02  (2 jours) AutoCAD 2002 - Level 1:  14, 15, 21, 22.11.02  (4 days) LabVIEW - Advanced (English) :  18 - 20.11.2002  (3 days) AutoCAD 2002 - niveau 1 :  19, 20, 25, 26.11.02 (4 jours) Oracle iDS Designer: First Class:&...

  12. Places available**

    CERN Document Server

    2003-01-01

    Places are available in the following courses: CLEAN-2002 : Travailler en salle blanche (séminaire gratuit) : 4.9.03 (une demi-journée) The CERN EDMS for Local Administrators (free of charge) : 24 & 25.9.03 (2 days) HeREF-2003 : Techniques de la réfrigération Hélium (cours en français avec support en anglais) : 6 - 10.10.2003 (7 demi-journées) The Java Programming Language Level 1 : 6 - 7.10.2003 (2 days) Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) AutoCAD 2002 - niveau 1 : 20, 21, 27, 28.10.03 (4 jours) CLEAN-2002 : Working in a Cleanroom (free of charge) : 23.10.03 (half day) AutoCAD Mechanical 6 PowerPack (E) : 23, 24, 30, 31.10 & 12, 13.11.03 (6 days) AutoCAD 2002 - niveau 2 : 10 & 11.11.03 (2 jours)...

  13. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: The CERN EDMS for Local Administrators : 24 & 25.9.03 (2 days, free of charge) HeREF-2003 : Techniques de la réfrigération Hélium cours en français avec support en anglais) : 6 - 10.10.2003 (7 demi-journées) The Java Programming Language Level 1 : 6 - 7.10.2003 (2 days) Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) AutoCAD 2002 - niveau 1 : 20, 21, 27, 28.10.03 (4 jours) CLEAN-2002 : Working in a Cleanroom : 23.10.03 (half day, free of charge) AutoCAD 2002 - Level 1 : 3, 4, 12, 13.11.03 (4 days) AutoCAD 2002 - niveau 2 : 10 & 11.11.03 (2 jours) ACCESS 2000 - niveau 1 : 13 & 14.11.03 (2 jours) AutoCAD Mechanical 6 PowerPack (E) : 17, 18, 24, 25.11 & 1, 2.12.03 (6 days) FrontPage 2000 - niveau 1 : 20 & 21.11.03 (2 jours) MAGNE-03 : Magnétisme pour l'électrotechnique : 25 - 27.11.03 (3 jours) ...

  14. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: LabView hands-on (bilingue/bilingual): 5.11.02 (matin/morning) LabView DAQ hands-on (bilingue/bilingual):  5.11.02  (après-midi afternoon) Introduction au PC et Windows 2000 au CERN:  6 & 7.11.02  (2 jours) Oracle 8i : Access the Database with Java:  7 & 8.11.02  (2 days) AutoCAD 2002 - niveau 2:  7 & 8.11.02  (2 jours) Introduction to PVSS (free of charge):  11.11.2002 pm  (1/2 day) Basic PVSS:  12 - 14.11.02  (3 days) EXCEL 2000 - niveau 1:  12 & 13.11.02  (2 jours) CLEAN-2002: Working in a Cleanroom (English, free of charge):  13.11.2002  (afternoon) LabView Base 1 :  13 - 15.11.02  (3 jours) AutoCAD 2002 - Level 1:  14, 15, 21, 22.11.2002  (4 days) LabVIEW - Advanced:  18 - 20.11.02  (3 days) Auto...

  15. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: PIPES-2003 - Pratique du sertissage de tubes métalliques et multicouches :26.8.03(stage pratique) The CERN EDMS for Engineers (free of charge) : 27.8.03 (1 day) CLEAN-2002 : Travailler en salle blanche (séminaire gratuit) : 4.9.03(une demi-journée) The CERN EDMS for Local Administrators (free of charge) : 24 & 25.9.03 (2 days) HeREF-2003 : Techniques de la réfrigération Hélium (cours en français avec support en anglais) : 6 - 10.10.2003 (7 demi-journées) The Java Programming Language Level 1 : 6 - 7.10.2003 (2 days) Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) AutoCAD 2002 - niveau 1 : 20, 21, 27, 28.10.03 (4 jours) CLEAN-2002 : Working in a Cleanroom (free of charge) : 23.10.03 (half day) AutoCAD Mechanical 6 PowerPack (E) : 23, 24, 30, 31.10 & 12, 13.11.03 (6 days) AutoCAD 2002 - niveau 2...

  16. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: The CERN EDMS for Local Administrators (free of charge) : 24 & 25.9.03 (2 days) HeREF-2003 : Techniques de la réfrigération Hélium (cours en français avec support en anglais) : 6 - 10.10.2003 (7 demi-journées) The Java Programming Language Level 1 : 6 - 7.10.2003 (2 days) Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) AutoCAD 2002 - niveau 1 : 20, 21, 27, 28.10.03 (4 jours) CLEAN-2002 : Working in a Cleanroom (free of charge) : 23.10.03 (half day) AutoCAD Mechanical 6 PowerPack (E) : 23, 24, 30, 31.10 & 12, 13.11.03 (6 days) AutoCAD 2002 - niveau 2 : 10 & 11.11.03 (2 jours) ACCESS 2000 - niveau 1 : 13 & 14.11.03 (2 jours) FrontPage 2000 - niveau 1 : 20...

  17. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: October 2002   Introduction to the CERN Engineering Data Management System (free of charge):  29.10.2002  (1 day) The CERN EDMS for Advanced users (free of charge):  30.10.2002  (1 day) November 2002   LabView hands-on (bilingue/bilingual): 5.11.02 (matin/morning) LabView DAQ hands-on (bilingue/bilingual):  5.11.02  (après-midi afternoon) Introduction au PC et Windows 2000 au CERN :  6 & 7.11.02  (2 jours) Oracle 8i : Access the Database with Java:  7 & 8.11.02  (2 days) AutoCAD 2002 - niveau 2 :  7 & 8.11.02  (2 jours) Introduction to PVSS (free of charge):  11.11.2002 pm  (1/2 day) Basic PVSS:  12 - 14.11.02  (3 days) EXCEL 2000 - niveau 1 :  12 & 13.11.02  (2 jours) CLEAN-2002: Working in a Cleanroom (English, free ...

  18. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Introduction to Oracle 8i : SQL and PL/SQL:  7 - 11.10.02  (5 days) CLEAN-2002 : Working in a Cleanroom (free of charge):  10.10.02  (half-day, p.m.) LabView Hands-on (bilingue/bilingual) : 10.10.02 (matin/morning) LabView DAQ Hands-on (bilingue/bilingual)  10.10.02 (après-midi /afternoon) Introduction à DesignSpace :  16.10.02  (1 journée) Introduction to DesignSpace:  17.10.02  (1 day) AutoCAD Mechanical 6 PowerPack (F) :  21, 22, 23.10 et 4, 5, 6.11.02  (6 jours) Introduction à ANSYS/Introduction to ANSYS (langue à définir suivant demande/ Language to be chosen according to demand):  21 - 25.10.02  (5 jours/days) HREF-2002: Helium Refrigeration Techniques (English-French, bilingual) :  21 - 25.10.2002  (7 half days) HREF-2002: Techniques de la...

  19. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel 74924

    2002-01-01

    Places are available in the following courses: LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 - 25.1.02 (4 jours) MS-Project 2000 : 24 & 25.01.02 (2 jours) Introduction au PC et à Windows 2000 au CERN : 29 - 30.1.02 (2 jours) LabView Base 1 : 4 - 6.2.02 (3 jours) LabView DAQ (E) : 7 & 8.02.02 (2 days) Hands-on Object-Oriented Design & Programming with Java : 11 - 13.02.02 (3 days) C++ for Particle Physicists : 11 - 15.3.2002 (6 * 3 hour lectures) Cours sur la migration AutoCAD : AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé sur AutoCAD 2002 (5 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO ...

  20. PLACES AVAILABLE

    CERN Multimedia

    Enseignement Technique; Tél. 74924; Technical Training; Monique Duval; Tel. 74924

    2000-01-01

    Places are available in the following courses : Premiers pas avec votre PC 12 - 15.9.00 (4 demi-journées) WORD 20, 21 et 26, 27.9.2000 (4 jours) JAVA programming level 1 25 - 26.9.2000 (2 days) Gaz inflammables 1 26.9.2000 (1 journée) Advanced aspects of PERL 5 6.10.2000 (1 day) Initiation au WWW 10 - 12.10.00 (3 demi-journées) WORD : importer et manipuler des images 16.10.2000 (1 journée) FileMaker 17, 18 et 24, 25.10.00 (4 jours) Nouveautés de WORD 19 et 20.10.2000 (2 jours) ACCESS 1er niveau 30 - 31.10.00 (2 jours)Introduction à PowerPoint 6.11.00 (1 journée)Nouveautés d’EXCEL 7.11.2000(4 demi-journées)Excel 13, 14 et 20, 21.11.00 (4 jours) LabView hands-on 13.11.2000(4 hours)LabView Basics 1 14 - 16.11.2000 (3 days) MS-Project 1er niveau 14-17.11.00 (4 demi-journées) If you wish to participate in one of these courses, please discuss with your supervisor and apply elec...

  1. PLACES AVAILABLE

    CERN Multimedia

    TECHNICAL TRAINING; Tel. 74460

    2001-01-01

    Places are available in the following courses: MS-Project 1er niveau : 20 - 23.2.01 (4 matins) Architecture d'automatisme : 20 - 21.2.01 (2 jours) Introduction à PowerPoint : 26.2.01 (1 journée) Programmation TSX Premium 1 (Schneider) : 26.2 - 2.3.01 (5 jours) Premiers pas avec votre PC : 27.2 - 2.3.01 (4 matins) C++ for Particle Physicists : 5 - 9.3.01 (6*3 hour lectures) EXCEL : 6, 7 et 13, 14.3.01 (4 jours) The JAVA programming language level 2 :  12 - 14.3.01 (3 days) Nouveautés de FileMaker :  20 - 23.03.01 (4 matins) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

  2. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2002-01-01

    Places are available in the following courses: LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 - 25.1.02 (4 jours) MS-Project 2000 : 22, 24 & 25.01.02 (3 jours) Introduction au PC et à Windows 2000 au CERN : 29 - 30.1.02 (2 jours) LabView Base 1 : 4 - 6.2.02 (3 jours) LabView DAQ  (E) :  7 & 8.02.02 (2 days) Hands-on Object-Oriented Design & Programming with Java :  11 - 13.02.02 (3 days) PVSS basics :  11 - 15.2.02 (5 days) Introduction à Windows 2000 : 18.2.02 (1 demi-journée) Introduction to the CERN Engineering Data Management System :  20.2.02 (1 day) The CERN Engineering Data Management System for Advanced users :  21.2.02  (1 day) C++ for Particle Physicists :  11 - 15.3.2002  (6 * 3 hour lectures) Cours sur la migration AutoCAD : AutoCAD : Mise à...

  3. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: LabVIEW - Basics 1 :  10 - 12.12.01 (3 days) Introduction to XML :  12 & 13.12.01 (2 days) Introduction au PC et Windows 2000 : 12 & 14.12.01 (2 jours) LabVIEW - Basics 2 :  13 - 14.12.01 (2 days) Habilitation électrique : superviseurs : 17.12.2001 (1/2 journée) MS-Project 2000 : 10 & 11.01.02 (2 jours) EXCEL 2000 - niveau 2 : 15 - 16.1.02 (2 jours) Sécurité dans les installations cryogéniques: 15-17.1.2002 (2 demi-journées) C++ Programming Level 2 - Traps and Pitfalls :  15 - 18.1.2002  (4 days) ELEC-2002 Winter Term: Readout and system electronics for Physics  15.1.2002 - 7.2.2002 (8 half- days) Nouveautés de WORD 2000 : 18.1.02 (1/2 journée) LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 -...

  4. PLACES AVAILABLE

    CERN Multimedia

    Enseignement Technique; Tel. 74924

    2001-01-01

    Places are available in the following courses: MS-Project 2000 : 10 & 11.01.02 (2 jours) EXCEL 2000 - niveau 2 : 15 - 16.1.02 (2 jours) Sécurité dans les installations cryogéniques: 15-17.1.2002 (2 demi-journées) C++ Programming Level 2 - Traps and Pitfalls :  15 - 18.1.2002  (4 days) ELEC-2002 Winter Term: Readout and system electronics for Physics  15.1.2002 - 7.2.2002 (8 half- days) Nouveautés de WORD 2000 : 18.1.02 (1/2 journée) LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 - 25.1.02 (4 jours) MS-Project 2000 : 24 & 25.01.02 (2 jours) Introduction au PC et à Windows 2000 au CERN : 29 - 30.1.02 (2 jours) LabView Base 1 : 4 - 6.2.02 (3 jours) LabView DAQ  (E) :  7 & 8.02.02 (2 days) Hands-on Object-Oriented Design & Programming with Java :&nbs...

  5. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : CLEAN-2002 : Working in a cleanroom (free course, registration required) : 2.4.03 (half-day, afternoon) LabView base 1/LabView Basics 1 (Langue à définir/ language to be decided) : 9 - 11.4.03 (3 jours/3 days) DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03(6 jours) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 31.3 - 4.4.03 / 16 - 20.6.03 (5 jours/5 days) Réseau Simatic Net /Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 These courses will be given in French or English following the requests. Cours de sécurité : Etre TSO au CERN : 3 sessions sont programmées pour 2003 : 25...

  6. Places available **

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : CLEAN-2002 : Working in a cleanroom (free course, registration required): 11.4.03 (half-day, afternoon, ) LabView Basics 2 : 10 - 11.4.03 (3 days) DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) Oracle iDS Reports : Build Internet Reports : 5 - 9.5.03 (5 days) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03 (6 jours) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 31.3 - 4.4.03 / 16 - 20.6.03 (5 jours/5 days) Réseau Simatic Net /Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 These courses will be given in French or English following the requests. Cours de sécurité : Etre TSO au CERN : Prochaines sessions : 24, 25 & 27.6....

  7. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) Oracle iDS Reports : Build Internet Reports : 5 - 9.5.03 (5 days) LabView DAQ (language to be defined) : 8 & 9.5.03 AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03 (6 jours) AutoCAD 2002 - niveau 2 : 3 & 4.6.03 (2 jours) LabView DSC (language to be defined) : 19 & 20.6.03 Siemens SIMATIC Training : Introduction to STEP7 : 3 & 4.6.03 (2 days) STEP7 Programming : 16 - 20.6.03 (5 days) Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 (sessions of 2 days) These courses will be given in French or English following the requests. Cours de sécurité : Etre TSO au CERN : Prochaines sessions : 24, 25 & 27.6.03 - 4, 5 & 7.11.03 (session de 3 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description ...

  8. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Habilitation électrique : recyclage HT/BT : 11 - 15.3.2002  (2 * 2 heures) PVSS Basics :  8 - 12.4.02  (5 days) ELEC-2002 : Spring Term :  9, 11, 16, 18, 23, 25, 30.4.02 (7 * 2.5 hours) LabVIEW base 1 : 22 - 24.4.02 (3 jours) LabVIEW DSC (F) 25 & 26.4.02 (2 jours) LabVIEW Basics 2 : 13 & 14.5.02 (2 days) LabVIEW DAQ (F) : 15 & 16.5.02 (2 jours) Cours sur la migration AutoCAD :   AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé sur AutoCAD 2002 (5 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applica...

  9. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : CLEAN-2002 : Working in a cleanroom (free course, registration required): 11.4.03 (half-day, afternoon, ) LabView Basics 2 : 10 - 11.4.03 (3 days) DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) Oracle iDS Reports : Build Internet Reports : 5 - 9.5.03 (5 days) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03(6 jours) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 31.3 - 4.4.03 / 16 - 20.6.03 (5 jours/5 days) Réseau Simatic Net /Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 These courses will be given in French or English following the requests. Cours de sécurité : Etre TSO au CERN : Prochaines sessions : 24, 25 & 27.6...

  10. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) Programmation de pilotes périphériques : 5 - 8.5.03 (4 jours) Oracle iDS Reports : Build Internet Reports : 5 - 9.5.03 (5 days) LabView DAQ (language to be defined) : 8 & 9.5.03 AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.0 (6 jours) FrontPage 2000 - niveau 1 : 20 & 21.5.03 (2 jours) AutoCAD 2002 - niveau 2 : 3 & 4.6.03 (2 jours) EXCEL 2000 - niveau 1 : 10 & 11.6.03 (2 jours) EXCEL 2000 - level 1 : 12 & 13.6.03 (2 days) PowerPoint 2000 (F) : 17 & 18.6.03 (2 jours) FrontPage 2000 - niveau 2 : 19 & 20.6.03 (2 jours) LabView DSC (langue à décider/language to be defined) : 19 & 20.6.03 EXCEL 2000 - niveau 2 : 24 & 25.6.03 (2 jours) Siemens SIMATIC Training : Introduction to STEP7 : 3 & 4.6.03 (2 days) STEP7 Programming : 16 - 20.6.03 (5 days) Simatic Net Network : 26 & 27.6.03 ...

  11. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : CLEAN-2002 : Working in a cleanroom (free course, registration required): 11.4.03 (half-day, afternoon) LabView Basics 2 : 10 - 11.4.03 (3 days) DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) Oracle iDS Reports : Build Internet Reports : 5 - 9.5.03 (5 days) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03(6 jours) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 16 - 20.6.03 (5 jours/5 days) Réseau Simatic Net /Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 These courses will be given in French or English following the requests. Cours de sécurité : Etre TSO au CERN : Prochaines sessions : 24, 25 & 27.6.03 - 4, 5 & 7....

  12. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : Introduction to PVSS : 10.3.03 (half-day, afternoon) CLEAN-2002 : Working in a cleanroom : 2.4.03 (half-day, afternoon, free course, registration required) LabView Basics 1 : 9 - 11.4.03 (3 days) Language to be decided. DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures). AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03 (6 jours) Siemens SIMATIC Training: Introduction to STEP7 : 3 & 4.6.03 (2 days) STEP7 Programming : 31.3 - 4.4.03 / 16 - 20.6.03 (5 days) Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 These courses will be given in French or English following the requests. Cours de sécurité: Etre TSO au CERN : 3 sessions sont programmées pour 2003 : 25, 26 & 28.3.03 - 24, 25 & 27.6.03 - 4, 5 & 7.11.03 (sessions de 3 jours) ** The number o...

  13. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: November 2002   Hands-on Object-Oriented Design and Programming with C++:  19 - 21.11.02  (3 days)  December 2002   LabVIEW - DSC (English) :  2 - 3.12.02  (2 days) AutoCAD 2002 - niveau 2 :  2 & 3.12.02  (2 jours) FileMaker (Français) :  2 - 5.12.02  (4 jours) PCAD Schémas - Débutants :  5 & 6.12.02  (2 jours) PCAD PCB - Débutants :  9 - 11.12.02  (3 jours) FrontPage 2000 - level 1:  9 & 10.12.02  (2 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisiona...

  14. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: November 2002   Java Programming Language level 1 :  28 & 29.11.02  (2 days) December 2002   LabVIEW - DSC (English) :  2 - 3.12.02  (2 days) FileMaker (Français) :  2 - 5.12.02  (4 jours) PCAD Schémas - Débutants :  5 & 6.12.02  (2 jours) PCAD PCB - Débutants :  9 - 11.12.02  (3 jours) FrontPage 2000 - level 1:  9 & 10.12.02  (2 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training M...

  15. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: November 2002   Introduction to PVSS (free of charge): 11.11.02  (afternoon) EXCEL 2000 - niveau 1 :  12 & 13.11.02  (2 jours) CLEAN-2002: Working in a Cleanroom (English, free of charge):  13.11.2002  (afternoon) AutoCAD 2002 - niveau 1 :  14, 15, 21, 22.11.02  (4 jours) Hands-on Object-Oriented Design and Programming with C++:  19 - 21.11.02  (3 days)  EXCEL 2000 - niveau 2 :  25 & 26.11.02  (2 jours) FrontPage 2000 - niveau 1 :  27 & 28.11.02  (2 jours) December 2002   LabVIEW - DSC (English) :  2 - 3.12.02  (2 days) AutoCAD 2002 - niveau 2 :  2 & 3.12.02  (2 jours) FileMaker (Français) :  2 - 5.12.02  (4 jours) PCAD Schémas - Débutants :  5 & 6.12.02 ...

  16. PLACES AVAILABLE

    CERN Multimedia

    Technical training; Tel. 74924

    2001-01-01

    Places are available in the following courses: PROFIBUS : 25 - 26.9.01 (2 jours) PROFIBUS : 27 - 28.9.01 (2 days) Automates et réseaux de terrain : 3 - 4.10.2001 (2 jours) PCAD Schémas - débutants : 4 - 5.10.01 (2 jours) PCAD PCB - débutants : 8 - 10.10.01 (3 jours) Programmation TSX Premium 1 : 15 - 19.10.01 (5 jours) Programmation TSX Premium 1 : 22 - 26.10.01 (5 jours) Programming TSX Premium 2: 19 - 23.11.01 (5 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) Autocad Migration support courses: a detailed calendar will be published shortly for this series of sessions which will start on 15.10.2001. Registration is already open AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé sur AutoCAD 2002 (5 jours) The following LabView courses will be given in either English or French according to demand LabVIEW - Base 1 / LabVIEW - Basics 1 : 10 - 12.9.01 (3 jours / 3 days)...

  17. PLACES AVAILABLES

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: PVSS Basics : 20 - 24.8.01 (5 days) PROFIBUS : 25 - 26.9.01 (2 jours) PROFIBUS : 27 - 28.9.01 (2 days) PCAD Schémas - débutants : 4 - 5.10.01 (2 jours) PCAD PCB - débutants : 8 - 10.10.01 (3 jours) Programming TSX Premium 1: 15 - 19.10.01 (5 days) Programmation TSX Premium 1 : 22 - 26.10.01 (5 jours) Programming TSX Premium 2: 19 - 23.11.01 (5 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) The following LabView courses will be given in either English or French according to demand LabVIEW - Base 1 / LabVIEW - Basics 1 : 10 - 12.9.01 (3 jours / 3 days) LabVIEW - DAQ / LabVIEW - DAQ : 13 - 14.9.01 (2 jours / 2 days) LabVIEW - Base 1 / LabVIEW - Basics 1 : 15 - 17.10.01 (3 jours / 3 days) LabVIEW - Base 2 / LabVIEW - Basics 2 : 18 - 19.10.01 (2 jours / 2 days) LabVIEW - Base 1 / LabVIEW - Basics 1 : 12 - 14.11.01 (3 jours / 3 days) LabVIEW - DAQ / LabVIEW - DAQ : 15 - 16.11.01 (2 jours / 2...

  18. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: November 2002   LabView hands-on (bilingue/bilingual): 5.11.02 (matin/morning) LabView DAQ hands-on (bilingue/bilingual):  5.11.02  (après-midi afternoon) PCAD Schémas - Débutants :  5 & 6.11.02  (2 jours) PCAD PCB - Débutants :  9 - 11.11.02  (3 jours) Introduction au PC et Windows 2000 au CERN :  6 & 7.11.02  (2 jours) Oracle 8i : Access the Database with Java :  7 & 8.11.02  (2 days) Introduction to PVSS (free of charge):  11.11.2002 pm  (1/2 day) Basic PVSS:  12 - 14.11.02  (3 days) EXCEL 2000 - niveau 1 :  12 & 13.11.02  (2 jours) CLEAN-2002: Working in a Cleanroom (English, free of charge):  13.11.2002  (afternoon) LabView Base 1 :  13 - 15.11.02  (3 jours) AutoCAD 2002 - niveau 1 :  14, 15, 21, 22.11.02  (4 jours) LabVIEW - Advanced:  18 - 20.11.02  (3 days) Hands-on Object-Oriented Design and Programming with C++ :  19 - 21.11.02  (3 days)  LabVIEW - Basics 2:  21 - 22.11.02 ...

  19. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : EXCEL 2000 - niveau 1 : 10 & 11.6.03 (2 jours) Conception de PCB rapides dans le flot Cadence : 11.6.03 (matin) EXCEL 2000 - level 1 : 12 & 13.6.03 (2 days) Introduction to PVSS : 16.6.03 (p.m.) Basic PVSS : 17 - 19.6.03 (3 days) Réalisation de PCB rapides dans le flot Cadence : 17.6.03 (matin) PVSS - JCOP Framework Tutorial : 20.6.03 (1 day) EXCEL 2000 - niveau 2 : 24 & 25.6.03 (2 jours) Siemens SIMATIC Training : Introduction to STEP7 : 3 & 4.6.03 (2 jours/2 days) STEP7 Programming : 16 - 20.6.03 (5 jours/5 days) Simatic Net Network : 26 & 27.6.03 (2 jours/2 days) These courses will be given in French or English following the requests. Programmation automate Schneider : Programmation automate Schneider TSX Premium - 1er niveau : 10 - 13.6.03 (4 jours) - audience : toute personne qui veux maitriser la msie en uvre et la programmation d'un automate TSX Premium - objectifs : maitriser la mise en uvre et la programmation d'un autom...

  20. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : FrontPage 2000 - niveau 1 : 20 & 21.5.03 (2 jours) PIPES-2003 : Pratique du sertissage de tubes métalliques et multicouches : 21.5.03 (1 jour) Introduction à la CAO Cadence : de la saisie de schéma Concept-HDL au PCB : 20 & 22.5.03 (2 jours) AutoCAD 2002 - niveau 2 : 3 & 4.6.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 5, 6, 12, 13, 26, 27.6.03 (6 jours) EXCEL 2000 - niveau 1 : 10 & 11.6.03 (2 jours) Conception de PCB rapides dans le flot Cadence : 11.6.03 (matin) EXCEL 2000 - level 1 : 12 & 13.6.03 (2 days) PowerPoint 2000 (F) : 17 & 18.6.03 (2 jours) Réalisation de PCB rapides dans le flot Cadence : 17.6.03 (matin) FrontPage 2000 - niveau 2 : 19 & 20.6.03 (2 jours) LabView DSC (langue à décider/language to be defined) : 19 & 20.6.03 EXCEL 2000 - niveau 2 : 24 & 25.6.03 (2 jours) Siemens SIMATIC Training: Introduction to STEP7 : 3 & 4.6.03 (2 days) STEP7 Programming : 16 - 20.6.03 (5 days) Simatic...

  1. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: PVSS Basics : 20 - 24.8.01 (5 days) PROFIBUS : 25 - 26.9.01 (2 jours) PROFIBUS : 27 - 28.9.01 (2 days) PCAD Schémas - débutants : 4 - 5.10.01 (2 jours) PCAD PCB - débutants : 8 - 10.10.01 (3 jours) Programming TSX Premium 1: 15 - 19.10.01 (5 days) Programmation TSX Premium 1 : 22 - 26.10.01 (5 jours) Programming TSX Premium 2: 19 - 23.11.01 (5 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) The following LabView courses will be given in either English or French according to demand LabVIEW - Base 1 / LabVIEW - Basics 1 : 10 - 12.9.01 (3 jours / 3 days) LabVIEW - DAQ / LabVIEW - DAQ : 13 - 14.9.01 (2 jours / 2 days) LabVIEW - Base 1 / LabVIEW - Basics 1 : 15 - 17.10.01 (3 jours / 3 days) LabVIEW - Base 2 / LabVIEW - Basics 2 : 18 - 19.10.01 (2 jours / 2 days) LabVIEW - Base 1 / LabVIEW - Basics 1 : 12 - 14.11.01 (3 jours / 3 days) LabVIEW - DAQ / LabVIEW - DAQ : 15 - 16.11.01 (2 jours / 2...

  2. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Introduction à Windows 2000 au CERN : 2 sessions de _ journée les 24 et 25.9.01 PROFIBUS : 25 - 26.9.01 (2 jours) PROFIBUS : 27 - 28.9.01 (2 days) PowerPoint 2000 : 1 et 2.10.01 (2 jours) EXCEL 2000 - niveau 1 : 3 et 4.10.01 (2 jours) Automates et réseaux de terrain : 3 - 4.10.2001 (2 jours) PCAD Schémas - débutants : 4 - 5.10.01 (2 jours) Introduction à Outlook : 5.10.01 (1 journée) Frontpage 2000 - niveau 1 : 8 et 9.10.01 (2 jours) PCAD PCB - débutants : 8 - 10.10.01 (3 jours) C++ for Particle Physicists : 8 - 12.10.01 (6 3-hour lectures) MS-Project 2000 - niveau 1 : 15 - 18.10.01 (4 demi-journées) LabView Basics 1 :  15 - 17.10.01  (3 days) Programmation TSX Premium 1 : 15 - 19.10.01 (5 jours) WORD 2000 : importer et manipuler des images : 19.10.01 (1 journée) Programmation TSX Premium 1 : 22 - 26.10.01...

  3. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Cadence Board Design tools : Upgrading to release 14 :  3 1-day sessions on 9, 10 & 11.10.01 MS-Project 2000 - niveau 1 : 15 - 18.10.01 (4 demi-journées) LabView Base 2 : 18 & 19.10.01 (2 jours) WORD 2000 : importer et manipuler des images : 19.10.01 (1 journée) Contract Follow-up (F) :  30.10.01 (1/2 journée) The CERN Engineering Data Management System for Electronics Design :  30.10.01 (1 day) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) The Java programming language Level 1: 8 - 9.11.01 (2 days) LabView Base 1 : 12 - 14.11.01 (3 jours) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to XML :  19 - 20.11.01 (2 days) Programming TSX Premium 1 :  19 - 23.11.01  (5 days) Introduction to C Programming :  21- 23.11.01 (3 days) The Java programming language Level 2:  26 - 28.11.01 (...

  4. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Introduction à Windows 2000 au CERN : 2 sessions de _ journée les 24 et 25.9.01 PROFIBUS : 25 - 26.9.01 (2 jours) PROFIBUS : 27 - 28.9.01 (2 days) EXCEL 2000 - niveau 1 : 3 et 4.10.01 (2 jours) Automates et réseaux de terrain : 3 - 4.10.2001 (2 jours) Introduction à Outlook : 5.10.01 (1 journée) Frontpage 2000 - niveau 1 : 8 et 9.10.01 (2 jours) C++ for Particle Physicists : 8 - 12.10.01 (6 lectures) MS-Project 2000 - niveau 1 : 15 - 18.10.01 (4 demi-journées) Programmation TSX Premium 1 : 15 - 19.10.01 (5 jours) WORD 2000 : importer et manipuler des images : 19.10.01 (1 journée) Programmation TSX Premium 1 : 22 - 26.10.01 (5 jours) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) The Java programming language Level 1: 8 - 9.11.01 (2 days) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to XML :  19 - 20.11.01 (2...

  5. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: EXCEL 2000 - niveau 1 : 3 et 4.10.01 (2 jours) Automates et réseaux de terrain : 3 - 4.10.2001 (2 jours) Introduction à Outlook : 5.10.01 (1 journée) C++ for Particle Physicists : 8 - 12.10.01 (6 lectures) Cadence Board Design tools : Upgrading to release 14 : 3 1-day sessions on 9, 10 & 11.10.01 MS-Project 2000 - niveau 1 : 15 - 18.10.01 (4 demi-journées) LabView Base 2 : 18 & 19.10.01 (2 jours) WORD 2000 : importer et manipuler des images : 19.10.01 (1 journée) The CERN Engineering Data Management System for Electronics Design :  30.10.01 (1 day) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) The Java programming language Level 1: 8 - 9.11.01 (2 days) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to XML :  19 - 20.11.01 (2 days) Programming TSX Premium 1 :  19 - 23.11.01  (5 days) Introd...

  6. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: MS-Project 2000 - niveau 1 : 15 - 18.10.01 (4 demi-journées) LabView Base 2 : 18 & 19.10.01 (2 jours) WORD 2000 : importer et manipuler des images : 19.10.01 (1 journée) Contract Follow-up (F) : 30.10.01 (1/2 journée) The CERN Engineering Data Management System for Electronics Design :  30.10.01 (1 day) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) The Java programming language Level 1: 8 - 9.11.01 (2 days) LabView Base 1 : 12 - 14.11.01 (3 jours) Automates et réseaux de terrain : 13 & 14.11.01 (2 jours) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to XML :  19 - 20.11.01 (2 days) Programming TSX Premium 1 :  19 - 23.11.01  (5 days) Introduction to C Programming :  21- 23.11.01 (3 days) The Java programming language Level 2:  26 - 28.11.01 (3 days) Programmation TSX Premium 2 : 26 ...

  7. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: LabView Base 1 :  23 - 25.9.02  (3 jours) Object-Oriented Analysis & Design using UML:  25 - 27.9.02  (3 days) LabView DAQ (E):  26 - 27.9.02  (2 days) Introduction to Oracle 8i : SQL and PL/SQL:  7 - 11.10.02  (5 days) CLEAN-2002 : Working in a Cleanroom (free of charge):  10.10.02  (half-day, p.m.) AutoCAD 2002 - niveau 2 :  14 - 15.10.02  (2 jours) Introduction à DesignSpace :  16.10.02  (1 journée) Introduction to DesignSpace:  17.10.02  (1 day) AutoCAD 2002 - Level 1:  17, 18, 24, 25.10.02  (4 days) AutoCAD Mechanical 6 PowerPack (F) :  21, 22, 23.10 et 4, 5, 6.11.02  (6 jours) Introduction à ANSYS/Introduction to ANSYS (langue à définir suivant demande/ Language to be chosen according to demand):...

  8. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Java 2 Enterprise Edition - Part 2: Enterprise JavaBeans:  18 - 20.9.02  (3 days) AutoCAD 2002 - niveau 1 :  19, 20, 26, 27.9.02  (4 jours) LabView Base 1 :  23 - 25.9.02  (3 jours) Object-Oriented Analysis & Design using UML:  25 - 27.9.02  (3 days) LabView DAQ (E):  26 - 27.9.02  (2 days) Introduction to Oracle 8i : SQL and PL/SQL:  7 - 11.10.02  (5 days) CLEAN-2002 : Working in a Cleanroom (free of charge):  10.10.02  (half-day, p.m.) AutoCAD 2002 - niveau 2 :  14 - 15.10.02  (2 jours) Introduction à DesignSpace :  16.10.02  (1 journée) Introduction to DesignSpace:  17.10.02  (1 day) AutoCAD 2002 - Level 1:  17, 18, 24, 25.10.02  (4 days) AutoCAD Mechanical 6 PowerPack (F) :  21, 22, 23.10 et 4, 5, 6.11....

  9. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Automates et réseaux de terrain : 13 & 14.11.01 (3 jours) Introduction à Windows 2000 au CERN : 12 - 14.11.01 (1/2 journée) Introduction to Windows 2000 at CERN :  14.11.01  (half-day) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Sécurité dans les installations cryogéniques : 21 - 22.11.2001 (2 demi-journées) Introduction to C Programming :  21- 23.11.01 (3 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) Contract Follow-up (F) : 26.11.01 (1/2 journée) Object-Oriented Analysis and Design :  27 - 30.11.2001  (4 days) Introduction to the CERN Engineering Data Management System :  30.11.2001 (1 day) Electromagnetic Compatibility (EMC): Introduction (bilingual) :  3.12.01 (half-day) Introduction to the CERN Engineering Data Management System : 07.12.2001...

  10. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Contract Follow-up (F) : 30.10.01 (1/2 journée) The CERN Engineering Data Management System for Electronics Design :  30.10.01 (1 day) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) Nouveautés d'EXCEL : 5.11.01 (1/2 journée) Introduction a Windows 2000 au CERN : 6.11.01 (1/2 journée) The Java programming language Level 1: 8 - 9.11.01 (2 days) LabView Base 1 : 12 - 14.11.01 (3 jours) Automates et réseaux de terrain : 13 & 14.11.01 (2 jours) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to XML :  19 - 20.11.01 (2 days) Programming TSX Premium 1 :  19 - 23.11.01  (5 days) Introduction to C Programming :  21- 23.11.01 (3 days) The Java programming language Level 2:  26 - 28.11.01 (3 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) Autocad Migration support courses: a detail...

  11. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Contract Follow-up (F) : 30.10.01 (1/2 journée) The CERN Engineering Data Management System for Electronics Design :  30.10.01 (1 day) Nouveautés d'Excel 2000 : 5.11.01 (1/2 journée) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) Introduction à Windows 2000 au CERN : 6.11.01 (1/2 journée) The Java programming language Level 1: 8 - 9.11.01 (2 days) LabView Base 1 : 12 - 14.11.01 (3 jours) LabVIEW DAQ (F) : 15 & 16.11.01 (2 jours) Automates et réseaux de terrain : 13 & 14.11.01 (2 jours) Introduction to PERL 5 :  15 - 16.11.01  (2 days) LabVIEW - DAQ : 15 - 16.11.01 (2 jours) Introduction to XML :  19 - 20.11.01 (2 days) Introduction to C Programming :  21- 23.11.01 (3 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) Object-Oriented Analysis and Design :  27 - 30.11.2001 (4 days) Hands...

  12. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Nouveautés d'EXCEL : 5.11.01 (1/2 journée) Introduction a Windows 2000 au CERN : 6.11.01 (1/2 journée) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) Design Patterns :  7 - 8.11.01 (2 days) The Java programming language Level 1: 8 - 9.11.01 (2 days) Automates et réseaux de terrain : 13 & 14.11.01 (3 jours) Introduction à Windows 2000 au CERN : 12 - 14.11.01 (1/2 journée) Introduction to Windows 2000 at CERN :  14.11.01  (half-day) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to C Programming :  21- 23.11.01 (3 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) Contract Follow-up (F) : 26.11.01 (1/2 journée) Object-Oriented Analysis and Design :  27 - 30.11.2001  (4 days) Hands-on Object-Oriented Design and Programming with C++ :  11 - 13.12.2...

  13. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: ELEC-2002 : Spring Term :  9, 11, 16, 18, 23, 25, 30.4.02 (7 * 2.5 hours) Object-Oriented Analysis & Design: 16 - 19.4.02  (4 days) The CERN Engineering Data Management System for Advanced users:  16.4.02  (1 day) Migration from AutoCAD 14 towards AutoCAD Mechanical6 PowerPack:  17 - 19.4 and 2 &3.5.02  (5 days) AutoCAD - niveau 1 : 22, 23, 29, 30.4 et 6, 7.5.02 (6 jours) LabVIEW base 1 : 22 - 24.4.02 (3 jours) CLEAN 2002 : working in a cleanroom:  24.4.02  (half-day, pm) LabVIEW DSC (F) 25 & 26.4.02 (2 jours) AutoCAD : Mise à jour AutoCAD r-14 vers 2002 : 25 & 26.4.02 (2 jours) Cotations selon les normes GPS de l'ISO : 29 - 30.4.02 (2 jours) Introduction to the CERN Engineering Data Management System:  7.5.02  (1 day) LabVIEW Basics 2 : 13 & 14.5.02 (2 days) AutoCAD Mechanical 6 PowerPack (F) : 13-...

  14. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Introduction to the CERN Engineering Data Management System:  7.5.02  (1 day) LabVIEW Basics 2: 13 & 14.5.02 (2 days) AutoCAD Mechanical 6 PowerPack (F) : 13-14, 17, 21, 27-28.5.02 (6 jours) WorldFIP - Généralités : 14.5.2002 (1/2 journée) WorldFIP - Développer avec MicroFIP HANDLER : 14.5 - après-midi, 15.5.02 - matin (1 jour) WorldFIP - FullFIP FDM : FIP Device Manager (F) : 15.5 - après-midi, 16.5.02 - matin (1 jour) LabVIEW DAQ (F) : 15 & 16.5.02 (2 jours) EXCEL 2000 - niveau 2 : 22 & 23.5.02 (2 jours) The CERN Engineering Data Management System for Advanced users:  30.5.02  (1 day) LabVIEW Basics 1:  3 - 5.6.02  (3 days) AutoCAD 2002 - condensé : 4 - 6.6.02 (3 jours) LabVIEW DAQ (E):  6 & 7.6.02  (2 days) AutoCAD 2002 - Level 1:  10 - ...

  15. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: The CERN Engineering Data Management System for Advanced users : 16.4.02  (1 day) Migration from AutoCAD 14 towards AutoCAD Mechanical6 PowerPack:  17 - 19.4 and 2 &3.5.02  (5 days) AutoCAD - niveau 1 : 22, 23, 29, 30.4 et 6, 7.5.02 (6 jours) LabVIEW base 1 : 22 - 24.4.02 (3 jours) CLEAN 2002 : working in a cleanroom:  24.4.02  (half-day, pm) LabVIEW DSC (F) 25 & 26.4.02 (2 jours) AutoCAD : Mise à jour AutoCAD r-14 vers 2002 : 25 & 26.4.02 (2 jours) Cotations selon les normes GPS de l'ISO : 29 - 30.4.02 (2 jours) Introduction to the CERN Engineering Data Management System:  7.5.02  (1 day) LabVIEW Basics 2: 13 & 14.5.02 (2 days) AutoCAD Mechanical 6 PowerPack (F) : 13-14, 17, 21, 27-28.5.02 (6 jours) WorldFIP - Généralités : 14.5.2002 (1/2 journée) WorldFIP - Développer avec Micr...

  16. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: LabVIEW Basics 2: 13 & 14.5.02 (2 days) WorldFIP - Généralités : 14.5.2002 (1/2 journée) WorldFIP - Développer avec MicroFIP HANDLER : 14.5 - après-midi, 15.5.02 - matin (1 jour) WorldFIP - FullFIP FDM : FIP Device Manager (F) : 15.5 - après-midi, 16.5.02 - matin (1 jour) LabVIEW DAQ (F) : 15 & 16.5.02 (2 jours) EXCEL 2000 - niveau 2 : 22 & 23.5.02 (2 jours) The CERN Engineering Data Management System for Advanced users:  30.5.02  (1 day) LabVIEW Basics 1:  3 - 5.6.02  (3 days) AutoCAD 2002 - condensé : 4 - 6.6.02 (3 jours) LabVIEW DAQ (E):  6 & 7.6.02  (2 days) AutoCAD 2002 - Level 1:  10 - 12 and 24 - 26.6.02  (6 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the c...

  17. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: LabVIEW base 1 : 22 - 24.4.02 (3 jours) CLEAN 2002 : working in a cleanroom:  24.4.02  (half-day, pm) LabVIEW DSC (F) 25 & 26.4.02 (2 jours) AutoCAD : Mise à jour AutoCAD r-14 vers 2002 : 25 & 26.4.02 (2 jours) Cotations selon les normes GPS de l'ISO : 29 - 30.4.02 (2 jours) Introduction to the CERN Engineering Data Management System:  7.5.02  (1 day) LabVIEW Basics 2: 13 & 14.5.02 (2 days) AutoCAD Mechanical 6 PowerPack (F) : 13-14, 17, 21, 27-28.5.02 (6 jours) WorldFIP - Généralités : 14.5.2002 (1/2 journée) WorldFIP - Développer avec MicroFIP HANDLER : 14.5 - après-midi, 15.5.02 - matin (1 jour) WorldFIP - FullFIP FDM : FIP Device Manager (F) : 15.5 - après-midi, 16.5.02 - matin (1 jour) LabVIEW DAQ (F) : 15 & 16.5.02 (2 jours) EXCEL 2000 - niveau 2 : 22 & 23.5.02 (2 jours)...

  18. ‘We are a community [but] that takes a certain amount of energy’: Exploring shared visions, social action, and resilience in place-based community-led energy initiatives

    International Nuclear Information System (INIS)

    Parkhill, K.A.; Shirani, F.; Butler, C.; Henwood, K.L.; Groves, C.; Pidgeon, N.F.

    2015-01-01

    Highlights: • We engage with conceptual characteristics of 3 community-led energy case studies. • We examine data from interviews to explore the issues community energy groups face. • Shared visions, social action and social resilience are important to community energy. • Creating and maintaining shared visions, social action and social resilience is extremely challenging. - Abstract: In UK energy policy, community-led energy initiatives are increasingly being imbued with transformative power to facilitate low carbon transitions. The ways that such expectations for communities are manifesting in practice remains, however, relatively poorly understood. In particular, key conceptual developments in unpacking what constitutes ‘community’ that highlight the significance of ‘place’ along with important characteristics, such as shared visions, collective social action, and resilience, have yet to be comprehensively explored in the context of community-led energy initiatives. This paper uses an interpretive stance to engage with these conceptual ideas about community and provides insights into the nature of community and its meaning for developing energy-related initiatives and realising the wider goals of energy policy. The paper draws on data from in-depth qualitative, longitudinal interviews undertaken in two residential communities and one purely workplace-based community, which are engaged in community energy initiatives. We argue that there are difficulties and ambiguities in creating shared visions, achieving social action, and developing resilience that are related to the specificities of community in place, but that all three characteristics are likely to be important for the making of sustainable places

  19. Brand new authentic places

    DEFF Research Database (Denmark)

    Stender, Marie

    the relation and interplay between the two. This study strives to fill this gap by ethnographically tracing the process from design to occupancy including the role of branding as a means to create authenticity. The concept of authenticity is often associated with old houses and neighbourhoods, but also in new......How are places and material surroundings ascribed with meaning when new residential neighbourhoods are designed, branded and taken into use? Existing research on housing, neighbourhoods and urban design tends to take the perspective of either the architect or the user rather than to explore...... neighbourhoods stories of authenticity seems to be of great importance giving value and identity to place and people. By way of design and branding new places are implied with notions of the real, the original and the unique referring to e.g. its historical past, architectural uniqueness, sustainability or sense...

  20. Time Series Analysis of the Bacillus subtilis Sporulation Network Reveals Low Dimensional Chaotic Dynamics.

    Science.gov (United States)

    Lecca, Paola; Mura, Ivan; Re, Angela; Barker, Gary C; Ihekwaba, Adaoha E C

    2016-01-01

    Chaotic behavior refers to a behavior which, albeit irregular, is generated by an underlying deterministic process. Therefore, a chaotic behavior is potentially controllable. This possibility becomes practically amenable especially when chaos is shown to be low-dimensional, i.e., to be attributable to a small fraction of the total systems components. In this case, indeed, including the major drivers of chaos in a system into the modeling approach allows us to improve predictability of the systems dynamics. Here, we analyzed the numerical simulations of an accurate ordinary differential equation model of the gene network regulating sporulation initiation in Bacillus subtilis to explore whether the non-linearity underlying time series data is due to low-dimensional chaos. Low-dimensional chaos is expectedly common in systems with few degrees of freedom, but rare in systems with many degrees of freedom such as the B. subtilis sporulation network. The estimation of a number of indices, which reflect the chaotic nature of a system, indicates that the dynamics of this network is affected by deterministic chaos. The neat separation between the indices obtained from the time series simulated from the model and those obtained from time series generated by Gaussian white and colored noise confirmed that the B. subtilis sporulation network dynamics is affected by low dimensional chaos rather than by noise. Furthermore, our analysis identifies the principal driver of the networks chaotic dynamics to be sporulation initiation phosphotransferase B (Spo0B). We then analyzed the parameters and the phase space of the system to characterize the instability points of the network dynamics, and, in turn, to identify the ranges of values of Spo0B and of the other drivers of the chaotic dynamics, for which the whole system is highly sensitive to minimal perturbation. In summary, we described an unappreciated source of complexity in the B. subtilis sporulation network by gathering

  1. The dev Operon Regulates the Timing of Sporulation during Myxococcus xanthus Development.

    Science.gov (United States)

    Rajagopalan, Ramya; Kroos, Lee

    2017-05-15

    Myxococcus xanthus undergoes multicellular development when starved. Thousands of rod-shaped cells coordinate their movements and aggregate into mounds in which cells differentiate into spores. Mutations in the dev operon impair development. The dev operon encompasses a clustered regularly interspaced short palindromic repeat-associated (CRISPR-Cas) system. Null mutations in devI , a small gene at the beginning of the dev operon, suppress the developmental defects caused by null mutations in the downstream devR and devS genes but failed to suppress defects caused by a small in-frame deletion in devT We provide evidence that the original mutant has a second-site mutation. We show that devT null mutants exhibit developmental defects indistinguishable from devR and devS null mutants, and a null mutation in devI suppresses the defects of a devT null mutation. The similarity of DevTRS proteins to components of the CRISPR-associated complex for antiviral defense (Cascade), together with our molecular characterization of dev mutants, support a model in which DevTRS form a Cascade-like subcomplex that negatively autoregulates dev transcript accumulation and prevents DevI overproduction that would strongly inhibit sporulation. Our results also suggest that DevI transiently inhibits sporulation when regulated normally. The mechanism of transient inhibition may involve MrpC, a key transcription factor, whose translation appears to be weakly inhibited by DevI. Finally, our characterization of a devI devS mutant indicates that very little exo transcript is required for sporulation, which is surprising since Exo proteins help form the polysaccharide spore coat. IMPORTANCE CRISPR-Cas systems typically function as adaptive immune systems in bacteria. The dev CRISPR-Cas system of M. xanthus has been proposed to prevent bacteriophage infection during development, but how dev controls sporulation has been elusive. Recent evidence supported a model in which DevR and DevS prevent

  2. Role of the alpha-glucanase Agn2p in ascus-wall endolysis following sporulation in fission yeast

    NARCIS (Netherlands)

    Dekker, Nick; van Rijssel, Jos; Distel, Ben; Hochstenbach, Frans

    2007-01-01

    During sporulation in the ascomyceteous fungus Schizosaccharomyces pombe, diploid cells undergo differentiation into asci containing four haploid ascospores, which are highly resistant to environmental stresses. Although the morphogenetic processes involved in ascospore formation have been studied

  3. Sporulation of Bacillus sphaericus 2297: an electron microscope study of crystal-like inclusion biogenesis and toxicity to mosquito larvae.

    Science.gov (United States)

    Kalfon, A; Charles, J F; Bourgouin, C; de Barjac, H

    1984-04-01

    Sporulation of Bacillus sphaericus strain 2297 in a synchronous liquid culture was studied by electron microscopy. The t0 of sporulation occurred 7 h after the beginning of the lag phase. Crystal-like inclusions first appeared at t2 and reached their final size between t5 and t6. The release of the spore/inclusion complex occurred at about t15 (22 h after inoculation). Toxicity against Culex pipiens larvae was related to sporulation and appeared during the early stages of sporulation. The LC50 (24 h) decreased about 10(5)-fold between t0-2 and t7, in correlation with the formation of crystalline inclusions. Heat resistance of spores appeared later than toxicity.

  4. Places disponibles/Places available

    CERN Multimedia

    2004-01-01

    Si vous désirez participer à l'un des cours suivants, veuillez en discuter avec votre superviseur et vous inscrire électroniquement en direct depuis les pages de description des cours dans le Web que vous trouvez à l'adresse : http://www.cern.ch/Training/ ou remplissez une « demande de formation » disponible auprès du Secrétariat de votre Division ou de votre DTO (Délégué divisionnaire à la formation). Les places seront attribuées dans l'ordre de réception des inscriptions. If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Off...

  5. The kinetics of oocyst shedding and sporulation in two immunologically distinct strains of Eimeria maxima, GS and M6.

    Science.gov (United States)

    Al-Badri, Riadh; Barta, John Robert

    2012-11-01

    The kinetics of oocyst shedding and sporulation of two immunologically distinct strains of Eimeria maxima (GS and M6) were compared. Both strains had a prepatent period of approximately 120 h followed by peak oocyst shedding at 144-150 h post inoculation. Mean total oocyst output determined for each strain demonstrated that the fecundity of the M6 strain (12.8 × 10(3) ± 1.95) of E. maxima was roughly twice that of the GS strain (6.9 × 10(3) ± 3.33) when inoculated at the rate of 1,000 infective oocysts per bird. The process of oocyst sporulation was followed by repetitive sampling of sporulating oocysts at 26 °C with aeration over a 138 hour period. Sporulation was divided into five morphologically distinguishable stages whose abundance peaked at the following times during sporulation: unsporulated oocysts at 0 h; sporoblast anlagen at 18 h; sporoblasts without sporocyst walls at 22 h; and sporocysts without mature sporozoites at 38 h. The time to 50 % sporulation of E. maxima oocysts observed in the present study was approximately 53 h for both strains and all viable oocysts had completed sporulation by 60 h. In the present study, the prepatent periods, duration of oocyst shedding, and the relative kinetics of sporulation of the GS and M6 strains of E. maxima were found to be virtually identical despite the immunological distinctiveness of these two parasite strains.

  6. Sporulation of Metarhizium anisopliae var. Acridum and Beauveria bassiana on Rhammatocerus schistocercoides under humid and dry conditions

    Directory of Open Access Journals (Sweden)

    Magalhães Bonifácio Peixoto

    2000-01-01

    Full Text Available The sporulation of the fungi Metarhizium anisopliae var. acridum and Beauveria bassiana in cadavers of the grasshopper Rhammatocerus schistocercoides was studied in dry and humid environments. Both fungi were equally virulent against R. schistocercoides. However, internally, M. anisopliae produced more conidia than B. bassiana at 53% and 75% relative humidity. Externally, there was no sporulation at 53% and 75% RH, and M. anisopliae produced more conidia than B. bassiana at 100% RH.

  7. Quaternary re-arrangement analysed by spectral enhancement: the interaction of a sporulation repressor with its antagonist.

    Science.gov (United States)

    Scott, D J; Leejeerajumnean, S; Brannigan, J A; Lewis, R J; Wilkinson, A J; Hoggett, J G

    1999-11-12

    The protein/protein interaction between SinI and SinR has been studied by analytical ultracentrifugation and gel electrophoresis in an attempt to understand how these proteins contribute to developmental control of sporulation in Bacillus subtilis. SinR was found to be tetrameric, while SinI was found to exist as monomers and dimers in a rapidly reversible equilibrium. Labelling of SinR by incorporating the tryptophan analogue 7-azatryptophan (7AW) into the protein in place of tryptophan shifts the UV absorbance spectrum, thus allowing selective monitoring of 7AWSinR at 315 nm using the UV absorption optics of the analytical ultracentrifuge. Selective monitoring of SinR in mixtures of SinR and SinI enables the binding and stoichiometry of the interaction to be investigated quantitatively and unambiguously. We demonstrate that the oligomeric forms of SinR and SinI re-arrange to form a tight 1:1 SinR:SinI complex, with no stable intermediate species. A fragment of SinR, SinR(1-69), which contains only the DNA-binding domain, was found to be monomeric, showing that the protein appears not to oligomerise in a similar manner to the Cro repressor, a protein with which it shares a marked structural similarity. Copyright 1999 Academic Press.

  8. A single amino acid mutation in Spo0A results in sporulation deficiency of Paenibacillus polymyxa SC2.

    Science.gov (United States)

    Hou, Xiaoyang; Yu, Xiaoning; Du, Binghai; Liu, Kai; Yao, Liangtong; Zhang, Sicheng; Selin, C; Fernando, W G D; Wang, Chengqiang; Ding, Yanqin

    2016-01-01

    Sporulating bacteria such as Bacillus subtilis and Paenibacillus polymyxa exhibit sporulation deficiencies during their lifetime in a laboratory environment. In this study, spontaneous mutants SC2-M1 and SC2-M2, of P. polymyxa SC2 lost the ability to form endospores. A global genetic and transcriptomic analysis of wild-type SC2 and spontaneous mutants was carried out. Genome resequencing analysis revealed 14 variants in the genome of SC2-M1, including three insertions and deletions (indels), 10 single nucleotide variations (SNVs) and one intrachromosomal translocation (ITX). There were nine variants in the genome of SC2-M2, including two indels and seven SNVs. Transcriptomic analysis revealed that 266 and 272 genes showed significant differences in expression in SC2-M1 and SC2-M2, respectively, compared with the wild-type SC2. Besides sporulation-related genes, genes related to exopolysaccharide biosynthesis (eps), antibiotic (fusaricidin) synthesis, motility (flgB) and other functions were also affected in these mutants. In SC2-M2, reversion of spo0A resulted in the complete recovery of sporulation. This is the first global analysis of mutations related to sporulation deficiency in P. polymyxa. Our results demonstrate that a SNV within spo0A caused the sporulation deficiency of SC2-M2 and provide strong evidence that an arginine residue at position 211 is essential for the function of Spo0A. Copyright © 2016 The Author(s). Published by Elsevier Masson SAS.. All rights reserved.

  9. Modeling heat resistance of Bacillus weihenstephanensis and Bacillus licheniformis spores as function of sporulation temperature and pH.

    Science.gov (United States)

    Baril, Eugénie; Coroller, Louis; Couvert, Olivier; Leguérinel, Ivan; Postollec, Florence; Boulais, Christophe; Carlin, Frédéric; Mafart, Pierre

    2012-05-01

    Although sporulation environmental factors are known to impact on Bacillus spore heat resistance, they are not integrated into predictive models used to calculate the efficiency of heating processes. This work reports the influence of temperature and pH encountered during sporulation on heat resistance of Bacillus weihenstephanensis KBAB4 and Bacillus licheniformis AD978 spores. A decrease in heat resistance (δ) was observed for spores produced either at low temperature, at high temperature or at acidic pH. Sporulation temperature and pH maximizing the spore heat resistance were identified. Heat sensitivity (z) was not modified whatever the sporulation environmental factors were. A resistance secondary model inspired by the Rosso model was proposed. Sporulation temperatures and pHs minimizing or maximizing the spore heat resistance (T(min(R)), T(opt(R)), T(max(R)), pH(min(R)) and pH(opt(R))) were estimated. The goodness of the model fit was assessed for both studied strains and literature data. The estimation of the sporulation temperature and pH maximizing the spore heat resistance is of great interest to produce spores assessing the spore inactivation in the heating processes applied by the food industry. Copyright © 2011 Elsevier Ltd. All rights reserved.

  10. Switch-like reprogramming of gene expression after fusion of multinucleate plasmodial cells of two Physarum polycephalum sporulation mutants

    Energy Technology Data Exchange (ETDEWEB)

    Walter, Pauline; Hoffmann, Xenia-Katharina; Ebeling, Britta; Haas, Markus; Marwan, Wolfgang, E-mail: wolfgang.marwan@ovgu.de

    2013-05-24

    Highlights: •We investigate reprogramming of gene expression in multinucleate single cells. •Cells of two differentiation control mutants are fused. •Fused cells proceed to alternative gene expression patterns. •The population of nuclei damps stochastic fluctuations in gene expression. •Dynamic processes of cellular reprogramming can be observed by repeated sampling of a cell. -- Abstract: Nonlinear dynamic processes involving the differential regulation of transcription factors are considered to impact the reprogramming of stem cells, germ cells, and somatic cells. Here, we fused two multinucleate plasmodial cells of Physarum polycephalum mutants defective in different sporulation control genes while being in different physiological states. The resulting heterokaryons established one of two significantly different expression patterns of marker genes while the plasmodial halves that were fused to each other synchronized spontaneously. Spontaneous synchronization suggests that switch-like control mechanisms spread over and finally control the entire plasmodium as a result of cytoplasmic mixing. Regulatory molecules due to the large volume of the vigorously streaming cytoplasm will define concentrations in acting on the population of nuclei and in the global setting of switches. Mixing of a large cytoplasmic volume is expected to damp stochasticity when individual nuclei deliver certain RNAs at low copy number into the cytoplasm. We conclude that spontaneous synchronization, the damping of molecular noise in gene expression by the large cytoplasmic volume, and the option to take multiple macroscopic samples from the same plasmodium provide unique options for studying the dynamics of cellular reprogramming at the single cell level.

  11. Switch-like reprogramming of gene expression after fusion of multinucleate plasmodial cells of two Physarum polycephalum sporulation mutants

    International Nuclear Information System (INIS)

    Walter, Pauline; Hoffmann, Xenia-Katharina; Ebeling, Britta; Haas, Markus; Marwan, Wolfgang

    2013-01-01

    Highlights: •We investigate reprogramming of gene expression in multinucleate single cells. •Cells of two differentiation control mutants are fused. •Fused cells proceed to alternative gene expression patterns. •The population of nuclei damps stochastic fluctuations in gene expression. •Dynamic processes of cellular reprogramming can be observed by repeated sampling of a cell. -- Abstract: Nonlinear dynamic processes involving the differential regulation of transcription factors are considered to impact the reprogramming of stem cells, germ cells, and somatic cells. Here, we fused two multinucleate plasmodial cells of Physarum polycephalum mutants defective in different sporulation control genes while being in different physiological states. The resulting heterokaryons established one of two significantly different expression patterns of marker genes while the plasmodial halves that were fused to each other synchronized spontaneously. Spontaneous synchronization suggests that switch-like control mechanisms spread over and finally control the entire plasmodium as a result of cytoplasmic mixing. Regulatory molecules due to the large volume of the vigorously streaming cytoplasm will define concentrations in acting on the population of nuclei and in the global setting of switches. Mixing of a large cytoplasmic volume is expected to damp stochasticity when individual nuclei deliver certain RNAs at low copy number into the cytoplasm. We conclude that spontaneous synchronization, the damping of molecular noise in gene expression by the large cytoplasmic volume, and the option to take multiple macroscopic samples from the same plasmodium provide unique options for studying the dynamics of cellular reprogramming at the single cell level

  12. Environmental factors affecting sporulation of Fuligo septica (Myxomycetes on sugar cane bagasse

    Directory of Open Access Journals (Sweden)

    Chiappeta Alda de Andrade

    2003-01-01

    Full Text Available The influence of environmental factors on sporulation of Fuligo septica (L. Wigg. and the abundance of this species on sugar cane bagasse (Saccharum officinarum L., stored outdoors was studied.In Northeastern Brazil, between January/1997 and January/1998, a total of 29 specimens were collected through monthly collections of aethalia. The relationships between the abundance of aethalia and rainfall, temperature, relative humidity of the air and insolation were studied. Results indicated that on the substrate analyzed, F. septica was an abundant species. Sporulation occurred in all seasons of the year, with a well-defined peak at the end of winter and beginning of spring (August/September,which was strongly influenced by rainfall.

  13. Measurement of cellular copper levels in Bacillus megaterium during exponential growth and sporulation.

    Science.gov (United States)

    Krueger, W B; Kolodziej, B J

    1976-01-01

    Both atomic absorption spectrophotometry (AAS) and neutron activation analysis have been utilized to determine cellular Cu levels in Bacillus megaterium ATCC 19213. Both methods were selected for their sensitivity to detection of nanogram quantities of Cu. Data from both methods demonstrated identical patterms of Cu uptake during exponenetial growth and sporulation. Late exponential phase cells contained less Cu than postexponential t2 cells while t5 cells contained amounts equivalent to exponential cells. The t11 phase-bright forespore containing cells had a higher Cu content than those of earlier time periods, and the free spores had the highest Cu content. Analysis of the culture medium by AAS corroborated these data by showing concomitant Cu uptake during exponential growth and into t2 postexponential phase of sporulation. From t2 to t4, Cu egressed from the cells followed by a secondary uptake during the maturation of phase-dark forespores into phase-bright forespores (t6--t9).

  14. Two dynamin-like proteins stabilize FtsZ rings during Streptomyces sporulation.

    Science.gov (United States)

    Schlimpert, Susan; Wasserstrom, Sebastian; Chandra, Govind; Bibb, Maureen J; Findlay, Kim C; Flärdh, Klas; Buttner, Mark J

    2017-07-25

    During sporulation, the filamentous bacteria Streptomyces undergo a massive cell division event in which the synthesis of ladders of sporulation septa convert multigenomic hyphae into chains of unigenomic spores. This process requires cytokinetic Z-rings formed by the bacterial tubulin homolog FtsZ, and the stabilization of the newly formed Z-rings is crucial for completion of septum synthesis. Here we show that two dynamin-like proteins, DynA and DynB, play critical roles in this process. Dynamins are a family of large, multidomain GTPases involved in key cellular processes in eukaryotes, including vesicle trafficking and organelle division. Many bacterial genomes encode dynamin-like proteins, but the biological function of these proteins has remained largely enigmatic. Using a cell biological approach, we show that the two Streptomyces dynamins specifically localize to sporulation septa in an FtsZ-dependent manner. Moreover, dynamin mutants have a cell division defect due to the decreased stability of sporulation-specific Z-rings, as demonstrated by kymographs derived from time-lapse images of FtsZ ladder formation. This defect causes the premature disassembly of individual Z-rings, leading to the frequent abortion of septum synthesis, which in turn results in the production of long spore-like compartments with multiple chromosomes. Two-hybrid analysis revealed that the dynamins are part of the cell division machinery and that they mediate their effects on Z-ring stability during developmentally controlled cell division via a network of protein-protein interactions involving DynA, DynB, FtsZ, SepF, SepF2, and the FtsZ-positioning protein SsgB.

  15. Effect of powdered spice treatments on mycelial growth, sporulation and production of aflatoxins by toxigenic fungi

    OpenAIRE

    Chalfoun,Sára Maria; Pereira,Marcelo Cláudio; Resende,Mario Lúcio V.; Angélico,Caroline Lima; Silva,Rozane Aparecida da

    2004-01-01

    The effect of ten powdered spice plants was evaluated at the concentration of 1, 2, 3 and 4% to observe the mycelial growth and sporulation of Aspergillus niger and Eurotium repens. The spices were added to the culture media PDA and CYA20S. Clove completely inhibited the mycelial growth of the tested fungi. The other spices: cinnamon, garlic, thyme, mint, anis, oregano and onion were, in a decreasing order, promising antifungals. Bay leaf and basil did not show a pronounced fungistatic effect...

  16. Crystal Structures of the SpoIID Lytic Transglycosylases Essential for Bacterial Sporulation.

    Science.gov (United States)

    Nocadello, Salvatore; Minasov, George; Shuvalova, Ludmilla S; Dubrovska, Ievgeniia; Sabini, Elisabetta; Anderson, Wayne F

    2016-07-15

    Bacterial spores are the most resistant form of life known on Earth and represent a serious problem for (i) bioterrorism attack, (ii) horizontal transmission of microbial pathogens in the community, and (iii) persistence in patients and in a nosocomial environment. Stage II sporulation protein D (SpoIID) is a lytic transglycosylase (LT) essential for sporulation. The LT superfamily is a potential drug target because it is active in essential bacterial processes involving the peptidoglycan, which is unique to bacteria. However, the absence of structural information for the sporulation-specific LT enzymes has hindered mechanistic understanding of SpoIID. Here, we report the first crystal structures with and without ligands of the SpoIID family from two community relevant spore-forming pathogens, Bacillus anthracis and Clostridium difficile. The structures allow us to visualize the overall architecture, characterize the substrate recognition model, identify critical residues, and provide the structural basis for catalysis by this new family of enzymes. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  17. Effect of UV-C irradiation on growth, sporulation and pathogenicity of cochliobolus sativus isolates

    International Nuclear Information System (INIS)

    Jawhar, M.; Arabi, M.I.E.

    1999-12-01

    More than 30 isolates of Cochliobolus sativus, the causal agent of common root rot disease; were collected from different regions of Syria. Seven of them were exposed to UV-C light for 40 or 60 h . at a dose rate of 2.52x10 -3 W/cm 2 . A significant increases in the mycelium growth and sporulation were detected (p<0.001). Within the studied range of UV wave length, these two parameters were increased upon increasing the period of exposure to UV-C light. The pathogenicity of four isolates was evaluated after 60 h. of UV irradiation. The response to UV irradiation varied among these isolates, and resulted in an increase in their virulence level (as assessed by evaluating disease severity on sub-crown internodes). Five barley genotypes possessing different levels of resistance to C. sativus were studied. Arabi Abiad was the most susceptible cultivar whereas, Taka 76 line was moderately susceptible. It is concluded that it is possible to implement the positive effect of low doses of UV-C in stimulating the sporulation of fungi, which are difficult to sporulate on artificial media. (author)

  18. Impact of Serine/Threonine Protein Kinases on the Regulation of Sporulation in Bacillus subtilis.

    Science.gov (United States)

    Pompeo, Frédérique; Foulquier, Elodie; Galinier, Anne

    2016-01-01

    Bacteria possess many kinases that catalyze phosphorylation of proteins on diverse amino acids including arginine, cysteine, histidine, aspartate, serine, threonine, and tyrosine. These protein kinases regulate different physiological processes in response to environmental modifications. For example, in response to nutritional stresses, the Gram-positive bacterium Bacillus subtilis can differentiate into an endospore; the initiation of sporulation is controlled by the master regulator Spo0A, which is activated by phosphorylation. Spo0A phosphorylation is carried out by a multi-component phosphorelay system. These phosphorylation events on histidine and aspartate residues are labile, highly dynamic and permit a temporal control of the sporulation initiation decision. More recently, another kind of phosphorylation, more stable yet still dynamic, on serine or threonine residues, was proposed to play a role in spore maintenance and spore revival. Kinases that perform these phosphorylation events mainly belong to the Hanks family and could regulate spore dormancy and spore germination. The aim of this mini review is to focus on the regulation of sporulation in B. subtilis by these serine and threonine phosphorylation events and the kinases catalyzing them.

  19. Dominant Epistasis Between Two Quantitative Trait Loci Governing Sporulation Efficiency in Yeast Saccharomyces cerevisiae

    Science.gov (United States)

    Bergman, Juraj; Mitrikeski, Petar T.

    2015-01-01

    Summary Sporulation efficiency in the yeast Saccharomyces cerevisiae is a well-established model for studying quantitative traits. A variety of genes and nucleotides causing different sporulation efficiencies in laboratory, as well as in wild strains, has already been extensively characterised (mainly by reciprocal hemizygosity analysis and nucleotide exchange methods). We applied a different strategy in order to analyze the variation in sporulation efficiency of laboratory yeast strains. Coupling classical quantitative genetic analysis with simulations of phenotypic distributions (a method we call phenotype modelling) enabled us to obtain a detailed picture of the quantitative trait loci (QTLs) relationships underlying the phenotypic variation of this trait. Using this approach, we were able to uncover a dominant epistatic inheritance of loci governing the phenotype. Moreover, a molecular analysis of known causative quantitative trait genes and nucleotides allowed for the detection of novel alleles, potentially responsible for the observed phenotypic variation. Based on the molecular data, we hypothesise that the observed dominant epistatic relationship could be caused by the interaction of multiple quantitative trait nucleotides distributed across a 60--kb QTL region located on chromosome XIV and the RME1 locus on chromosome VII. Furthermore, we propose a model of molecular pathways which possibly underlie the phenotypic variation of this trait. PMID:27904371

  20. Dimer formation and transcription activation in the sporulation response regulator Spo0A.

    Science.gov (United States)

    Lewis, Richard J; Scott, David J; Brannigan, James A; Ladds, Joanne C; Cervin, Marguerite A; Spiegelman, George B; Hoggett, James G; Barák, Imrich; Wilkinson, Anthony J

    2002-02-15

    The response regulator Spo0A is the master control element in the initiation of sporulation in Bacillus subtilis. Like many other multi-domain response regulators, the latent activity of the effector, C-terminal domain is stimulated by phosphorylation on a conserved aspartic acid residue in the regulatory, N-terminal domain. If a threshold concentration of phosphorylated Spo0A is achieved, the transcription of genes required for sporulation is activated, whereas the genes encoding stationary phase sentinels are repressed, and sporulation proceeds. Despite detailed genetic, biochemical and structural characterisation, it is not understood how the phosphorylation signal in the receiver domain is transduced into DNA binding and transcription activation in the distal effector domain. An obstacle to our understanding of Spo0A function is the uncertainty concerning changes in quaternary structure that accompany phosphorylation. Here we have revisited this question and shown unequivocally that Spo0A forms dimers upon phosphorylation and that the subunit interactions in the dimer are mediated principally by the receiver domain. Purified dimers of two mutants of Spo0A, in which the phosphorylatable aspartic acid residue has been substituted, activate transcription from the spoIIG promoter in vitro, whereas monomers do not. This suggests that dimers represent the activated form of Spo0A. Copyright 2002 Elsevier Science Ltd.

  1. In-planta Sporulation Capacity Enhances Infectivity and Rhizospheric Competitiveness of Frankia Strains.

    Science.gov (United States)

    Cotin-Galvan, Laetitia; Pozzi, Adrien C; Schwob, Guillaume; Fournier, Pascale; Fernandez, Maria P; Herrera-Belaroussi, Aude

    2016-01-01

    Frankia Sp+ strains maintain their ability to sporulate in symbiosis with actinorhizal plants, producing abundant sporangia inside host plant cells, in contrast to Sp- strains, which are unable to perform in-planta sporulation. We herein examined the role of in-planta sporulation in Frankia infectivity and competitiveness for root infection. Fifteen strains belonging to different Sp+ and Sp- phylogenetic lineages were inoculated on seedlings of Alnus glutinosa (Ag) and A. incana (Ai). Strain competitiveness was investigated by performing Sp-/Sp+ co-inoculations. Plant inoculations were standardized using crushed nodules obtained under laboratory-controlled conditions (same plant species, age, and environmental factors). Specific oligonucleotide primers were developed to identify Frankia Sp+ and/or Sp- strains in the resulting nodules. Single inoculation experiments showed that (i) infectivity by Sp+ strains was significantly greater than that by Sp- strains, (ii) genetically divergent Sp+ strains exhibited different infective abilities, and (iii) Sp+ and Sp- strains showed different host preferences according to the origin (host species) of the inocula. Co-inoculations of Sp+ and Sp- strains revealed the greater competitiveness of Sp+ strains (98.3 to 100% of Sp+ nodules, with up to 15.6% nodules containing both Sp+ and Sp- strains). The results of the present study highlight differences in Sp+/Sp- strain ecological behaviors and provide new insights to strengthen the obligate symbiont hypothesis for Sp+ strains.

  2. Rap phosphatase of virulence plasmid pXO1 inhibits Bacillus anthracis sporulation.

    Science.gov (United States)

    Bongiorni, Cristina; Stoessel, Ricarda; Shoemaker, Dorinda; Perego, Marta

    2006-01-01

    This study shows that the Bacillus anthracis pXO1 virulence plasmid carries a Rap-Phr system, BXA0205, which regulates sporulation initiation in this organism. The BXA0205Rap protein was shown to dephosphorylate the Spo0F response regulator intermediate of the phosphorelay signal transduction system that regulates the initiation of the developmental pathway in response to environmental, metabolic, and cell cycle signals. The activity of the Rap protein was shown to be inhibited by the carboxy-terminal pentapeptide generated through an export-import processing pathway from the associated BXA0205Phr protein. Deregulation of the Rap activity by either overexpression or lack of the Phr pentapeptide resulted in severe inhibition of sporulation. Five additional Rap-Phr encoding systems were identified on the chromosome of B. anthracis, one of which, BA3790-3791, also affected sporulation initiation. The results suggest that the plasmid-borne Rap-Phr system may provide a selective advantage to the virulence of B. anthracis.

  3. What is taking place in science classrooms?: A case study analysis of teaching and learning in seventh-grade science of one Alabama school and its impact on African American student learning

    Science.gov (United States)

    Norman, Lashaunda Renea

    This qualitative case study investigated the teaching strategies that improve science learning of African American students. This research study further sought the extent the identified teaching strategies that are used to improve African American science learning reflect culturally responsive teaching. Best teaching strategies and culturally responsive teaching have been researched, but there has been minimal research on the impact that both have on science learning, with an emphasis on the African American population. Consequently, the Black-White achievement gap in science persists. The findings revealed the following teaching strategies have a positive impact on African American science learning: (a) lecture-discussion, (b) notetaking, (c) reading strategies, (d) graphic organizers, (e) hands-on activities, (f) laboratory experiences, and (g) cooperative learning. Culturally responsive teaching strategies were evident in the seventh-grade science classrooms observed. Seven themes emerged from this research data: (1) The participating teachers based their research-based teaching strategies used in the classroom on all of the students' learning styles, abilities, attitudes towards science, and motivational levels about learning science, with no emphasis on the African American student population; (2) The participating teachers taught the state content standards simultaneously using the same instructional model daily, incorporating other content areas when possible; (3) The participating African American students believed their seventh-grade science teachers used a variety of teaching strategies to ensure science learning took place, that science learning was fun, and that science learning was engaging; (4) The participating African American students genuinely liked their teacher; (5) The participating African American students revealed high self-efficacy; (6) The African American student participants' parents value education and moved to Success Middle School

  4. Sporulation boundaries and spore formation kinetics of Bacillus spp. as a function of temperature, pH and a(w).

    Science.gov (United States)

    Baril, Eugénie; Coroller, Louis; Couvert, Olivier; El Jabri, Mohammed; Leguerinel, Ivan; Postollec, Florence; Boulais, Christophe; Carlin, Frédéric; Mafart, Pierre

    2012-10-01

    Sporulation niches in the food chain are considered as a source of hazard and are not clearly identified. Determining the sporulation environmental boundaries could contribute to identify potential sporulation niches. Spore formation was determined in a Sporulation Mineral Buffer. The effect of incubation temperature, pH and water activity on time to one spore per mL, maximum sporulation rate and final spore concentration was investigated for a Bacillus weihenstephanensis and a Bacillus licheniformis strain. Sporulation boundaries of B. weihenstephanensis and of B. licheniformis were similar to, or included within, the range of temperatures, pH and water activities supporting growth. For instance, sporulation boundaries of B. weihenstephanensis were evaluated at 5°C, 35°C, pH 5.2 and a(w) 0.960 while growth boundaries were observed at 5°C, 37°C, pH 4.9 and a(w) 0.950. Optimum spore formation was determined at 30°C pH 7.2 for B. weihenstephanensis and at 45°C pH 7.2 for B. licheniformis. Lower temperatures and pH delayed the sporulation process. For instance, the time to one spore per mL was tenfold longer when sporulation occurred at 10°C and 20°C, for each strain respectively, than at optimum sporulation temperature. The relative effect of temperature and pH on sporulation rates and on growth rates is similar. This work suggests that the influence of environmental factors on the quantitative changes in sporulation boundaries and rates was similar to their influence on changes in growth rate. Copyright © 2012 Elsevier Ltd. All rights reserved.

  5. Effect of tcdR Mutation on Sporulation in the Epidemic Clostridium difficile Strain R20291.

    Science.gov (United States)

    Girinathan, Brintha P; Monot, Marc; Boyle, Daniel; McAllister, Kathleen N; Sorg, Joseph A; Dupuy, Bruno; Govind, Revathi

    2017-01-01

    Clostridium difficile is an important nosocomial pathogen and the leading cause of hospital-acquired diarrhea. Antibiotic use is the primary risk factor for the development of C. difficile -associated disease because it disrupts normally protective gut flora and enables C. difficile to colonize the colon. C. difficile damages host tissue by secreting toxins and disseminates by forming spores. The toxin-encoding genes, tcdA and tcdB , are part of a pathogenicity locus, which also includes the tcdR gene that codes for TcdR, an alternate sigma factor that initiates transcription of tcdA and tcdB genes. We created a tcdR mutant in epidemic-type C. difficile strain R20291 in an attempt to identify the global role of tcdR . A site-directed mutation in tcdR affected both toxin production and sporulation in C. difficile R20291. Spores of the tcdR mutant were more heat sensitive than the wild type (WT). Nearly 3-fold more taurocholate was needed to germinate spores from the tcdR mutant than to germinate the spores prepared from the WT strain. Transmission electron microscopic analysis of the spores also revealed a weakly assembled exosporium on the tcdR mutant spores. Accordingly, comparative transcriptome analysis showed many differentially expressed sporulation genes in the tcdR mutant compared to the WT strain. These data suggest that regulatory networks of toxin production and sporulation in C. difficile strain R20291 a re linked with each other. IMPORTANCE C. difficile infects thousands of hospitalized patients every year, causing significant morbidity and mortality. C. difficile spores play a pivotal role in the transmission of the pathogen in the hospital environment. During infection, the spores germinate, and the vegetative bacterial cells produce toxins that damage host tissue. Thus, sporulation and toxin production are two important traits of C. difficile . In this study, we showed that a mutation in tcdR , the toxin gene regulator, affects both toxin

  6. Take heart!

    CERN Multimedia

    Alizée Dauvergne

    2010-01-01

    Recently, ten new semi-automatic defibrillators were installed at various locations around CERN. This is a preventive measure intended to provide cardiac arrest victims with the best possible response. The first responder could be you!   The Director-General has welcomed the initiative of the Medical Service and Fire Brigade for the installation of ten new semi-automatic defibrillators. You have probably seen them on your way to the restaurant, for example:  brand new semi-automatic defibrillators, ready for an emergency. Housed in a white wall-mounted case, the bright red defibrillators are marked with a white heart symbol crossed by a lightning bolt (see photo). The defibrillator is designed so that anyone can use it. “Anyone can use it, you don’t need to be a health professional,” says Dr Reymond from CERN's Medical Service. Together with the CERN Fire Brigade, he is behind the initiative to have these units put in place. And with good reason, as the unit...

  7. Taking Leave?

    CERN Multimedia

    2000-01-01

    Planning a holiday? Then if you're a member of the personnel, you'll need to use the Laboratory's new leave system that will be put in place on 1 October. Leave allocations don't change - you are entitled to just as much holiday as before - but instead of being credited annually, your leave will be credited on a monthly basis, and this information will be communicated on your salary slip. The reason for the change is that with the various new leave schemes such as Recruitment by Saved Leave (RSL) and the Progressive Retirement Programme (PRP), a streamlined procedure was required for dealing with all kinds of leave. In the new system, each member of the personnel will have leave accounts to which leave will be credited monthly from the payroll and debited each time an absence is registered in the CERN Electronic Document Handling system (EDH). Leave balances will appear on monthly pay slips, and full details of leave transactions and balances will be available through EDH at all times. As the leave will be c...

  8. Tolerance Induction of Temperature and Starvation with Tricalcium Phosphate on Preservation and Sporulation in Bacillus amyloliquefaciens Detected by Flow Cytometry.

    Science.gov (United States)

    Shahrokh Esfahani, Samaneh; Emtiazi, Giti; Shafiei, Rasoul; Ghorbani, Najmeh; Zarkesh Esfahani, Seyed Hamid

    2016-09-01

    The Bacillus species have many applications in the preparation of various enzymes, probiotic, biofertilizer, and biomarkers for which the survival of resting cells and spore formation under different conditions are important. In this study, water and saline along with different mineral substances such as calcium carbonate, calcium phosphate, and silica were used for the detection of survival and preservation of Bacillus amyloliquefaciens. The results showed intensive death of resting cells at 8 °C, but significant survival at 28 °C after one month. However, preservation by minerals significantly decreased the rate of death and induced sporulation at both the temperatures. The resting cells were maintained at room temperature (about 60 % of the initial population survived after a month) in the presence of tricalcium phosphate. The results showed that temperature has more effect on sporulation compare with starvation. The sporulation in normal saline at 28 °C was 70 times more than that at 8 °C; meanwhile, addition of tricalcium phosphate increases sporulation by 90 times. Also, the FTIR data showed the interaction of tricalcium phosphate with spores and resting cells. The discrimination of sporulation from non-sporulation state was performed by nucleic acid staining with thiazole orange and detected by flow cytometry. The flow cytometric studies confirmed that the rates of sporulation in pure water were significantly more at 28 °C. This is the first report on the detection of bacterial spore with thiazole orange by flow cytometry and also on the interaction of tricalcium phosphate with spores by FTIR analyses.

  9. Two-Component Signal Transduction Systems That Regulate the Temporal and Spatial Expression of Myxococcus xanthus Sporulation Genes.

    Science.gov (United States)

    Sarwar, Zaara; Garza, Anthony G

    2016-02-01

    When starved for nutrients, Myxococcus xanthus produces a biofilm that contains a mat of rod-shaped cells, known as peripheral rods, and aerial structures called fruiting bodies, which house thousands of dormant and stress-resistant spherical spores. Because rod-shaped cells differentiate into spherical, stress-resistant spores and spore differentiation occurs only in nascent fruiting bodies, many genes and multiple levels of regulation are required. Over the past 2 decades, many regulators of the temporal and spatial expression of M. xanthus sporulation genes have been uncovered. Of these sporulation gene regulators, two-component signal transduction circuits, which typically contain a histidine kinase sensor protein and a transcriptional regulator known as response regulator, are among the best characterized. In this review, we discuss prototypical two-component systems (Nla6S/Nla6 and Nla28S/Nla28) that regulate an early, preaggregation phase of sporulation gene expression during fruiting body development. We also discuss orphan response regulators (ActB and FruA) that regulate a later phase of sporulation gene expression, which begins during the aggregation stage of fruiting body development. In addition, we summarize the research on a complex two-component system (Esp) that is important for the spatial regulation of sporulation. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  10. [Sporulation or competence development? A genetic regulatory network model of cell-fate determination in Bacillus subtilis].

    Science.gov (United States)

    Lu, Zhenghui; Zhou, Yuling; Zhang, Xiaozhou; Zhang, Guimin

    2015-11-01

    Bacillus subtilis is a generally recognized as safe (GRAS) strain that has been widely used in industries including fodder, food, and biological control. In addition, B. subtilis expression system also plays a significant role in the production of industrial enzymes. However, its application is limited by its low sporulation frequency and transformation efficiency. Immense studies have been done on interpreting the molecular mechanisms of sporulation and competence development, whereas only few of them were focused on improving sporulation frequency and transformation efficiency of B. subtilis by genetic modification. The main challenge is that sporulation and competence development, as the two major developmental events in the stationary phase of B. subtilis, are regulated by the complicated intracellular genetic regulatory systems. In addition, mutual regulatory mechanisms also exist in these two developmental events. With the development of genetic and metabolic engineering, constructing genetic regulatory networks is currently one of the most attractive research fields, together with the genetic information of cell growth, metabolism, and development, to guide the industrial application. In this review, the mechanisms of sporulation and competence development of B. subtilis, their interactions, and the genetic regulation of cell growth were interpreted. In addition, the roles of these regulatory networks in guiding basic and applied research of B. subtilis and its related species were discussed.

  11. Neurotoxin synthesis is positively regulated by the sporulation transcription factor Spo0A in Clostridium botulinum type E.

    Science.gov (United States)

    Mascher, Gerald; Mertaoja, Anna; Korkeala, Hannu; Lindström, Miia

    2017-10-01

    Clostridium botulinum produces the most potent natural toxin, the botulinum neurotoxin (BoNT), probably to create anaerobiosis and nutrients by killing the host, and forms endospores that facilitate survival in harsh conditions and transmission. Peak BoNT production coincides with initiation of sporulation in C. botulinum cultures, which suggests common regulation. Here, we show that Spo0A, the master regulator of sporulation, positively regulates BoNT production. Insertional inactivation of spo0A in C. botulinum type E strain Beluga resulted in significantly reduced BoNT production and in abolished or highly reduced sporulation in relation to wild-type controls. Complementation with spo0A restored BoNT production and sporulation. Recombinant DNA-binding domain of Spo0A directly bound to a putative Spo0A-binding box (CTTCGAA) within the BoNT/E operon promoter, demonstrating direct regulation. Spo0A is the first neurotoxin regulator reported in C. botulinum type E. Unlike other C. botulinum strains that are terrestrial and employ the alternative sigma factor BotR in directing BoNT expression, C. botulinum type E strains are adapted to aquatic ecosystems, possess distinct epidemiology and lack BotR. Our results provide fundamental new knowledge on the genetic control of BoNT production and demonstrate common regulation of BoNT production and sporulation, providing a key intervention point for control. © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.

  12. Comparative Transcriptomic and Proteomic Analyses Reveal a FluG-Mediated Signaling Pathway Relating to Asexual Sporulation of Antrodia camphorata.

    Science.gov (United States)

    Li, Hua-Xiang; Lu, Zhen-Ming; Zhu, Qing; Gong, Jin-Song; Geng, Yan; Shi, Jin-Song; Xu, Zheng-Hong; Ma, Yan-He

    2017-09-01

    Medicinal mushroom Antrodia camphorata sporulate large numbers of arthroconidia in submerged fermentation, which is rarely reported in basidiomycetous fungi. Nevertheless, the molecular mechanisms underlying this asexual sporulation (conidiation) remain unclear. Here, we used comparative transcriptomic and proteomic approaches to elucidate possible signaling pathway relating to the asexual sporulation of A. camphorata. First, 104 differentially expressed proteins and 2586 differential cDNA sequences during the culture process of A. camphorata were identified by 2DE and RNA-seq, respectively. By applying bioinformatics analysis, a total of 67 genes which might play roles in the sporulation were obtained, and 18 of these genes, including fluG, sfgA, SfaD, flbA, flbB, flbC, flbD, nsdD, brlA, abaA, wetA, ganB, fadA, PkaA, veA, velB, vosA, and stuA might be involved in a potential FluG-mediated signaling pathway. Furthermore, the mRNA expression levels of the 18 genes in the proposed FluG-mediated signaling pathway were analyzed by quantitative real-time PCR. In summary, our study helps elucidate the molecular mechanisms underlying the asexual sporulation of A. camphorata, and provides also useful transcripts and proteome for further bioinformatics study of this valuable medicinal mushroom. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. Missense Mutations Allow a Sequence-Blind Mutant of SpoIIIE to Successfully Translocate Chromosomes during Sporulation.

    Science.gov (United States)

    Bose, Baundauna; Reed, Sydney E; Besprozvannaya, Marina; Burton, Briana M

    2016-01-01

    SpoIIIE directionally pumps DNA across membranes during Bacillus subtilis sporulation and vegetative growth. The sequence-reading domain (γ domain) is required for directional DNA transport, and its deletion severely impairs sporulation. We selected suppressors of the spoIIIEΔγ sporulation defect. Unexpectedly, many suppressors were intragenic missense mutants, and some restore sporulation to near-wild-type levels. The mutant proteins are likely not more abundant, faster at translocating DNA, or sequence-sensitive, and rescue does not involve the SpoIIIE homolog SftA. Some mutants behave differently when co-expressed with spoIIIEΔγ, consistent with the idea that some, but not all, variants may form mixed oligomers. In full-length spoIIIE, these mutations do not affect sporulation, and yet the corresponding residues are rarely found in other SpoIIIE/FtsK family members. The suppressors do not rescue chromosome translocation defects during vegetative growth, indicating that the role of the γ domain cannot be fully replaced by these mutations. We present two models consistent with our findings: that the suppressors commit to transport in one arbitrarily-determined direction or delay spore development. It is surprising that missense mutations somehow rescue loss of an entire domain with a complex function, and this raises new questions about the mechanism by which SpoIIIE pumps DNA and the roles SpoIIIE plays in vivo.

  14. ISOLATION, CHARACTERIZATION MORPHOLOGICAL AND EVALUATION OF MYCELIAL GROWTH AND SPORULATION OF VARIOUS MEANS CULTURE OF Quambalaria sp.

    Directory of Open Access Journals (Sweden)

    Ediellen Mayara Corrêa Gomes

    2016-12-01

    Full Text Available The characterization of microorganisms is strategic for plant protection. The objective was to analyze the morphological characteristics and the mycelial growth and sporulation on different culture media fungus Quambalaria sp. Morphological characterization was based on color, type of colonies, type and size of spores were used taxonomic keys and specialized literature for description and identification of fungal structures. For the assessment of mycelial growth and sporulation were used means potato dextrose agar culture (PDA, vegetable-agar (V8 agar and oatmeal agar (OA. two isolates were obtained denominated Q1 and Q2 showed that different types of colonies and mycelial growth, and the spores of both characterized as hyaline, miniature format with obovoid the fusiform and its chambered hyphae. A linear relationship between sporulation and mycelial growth was observed, that is, the larger the sporulation, the greater mycelial growth. The means of V8-agar culture was the most stimulated mycelial growth and sporulation isolated Q1 and the BDA through the isolated Q2, with the MIGS of 11.07 and 10.57 mm day-1, respectively. The results of this study allow us to base the start of a study on the Quambalaria gender, in northern Brazil and to add to the few reported studies on this pathosystem. Keywords: leaf spot; eucalyptus; phytopathogen; forest pathology.

  15. Influence of culture media and environmental factors on mycelial growth and sporulation of Lasiodiplodia theobromae (Pat.) Griffon and Maubl.

    Science.gov (United States)

    Saha, A; Mandal, P; Dasgupta, S; Saha, D

    2008-05-01

    Lasiodiplodia theobromae, a common tea (Camellia sinensis) pathogen, usually does not sporulate or sporulates poorly in common media, which makes spore production difficult. In this study the effects of culture media, carbon source, nitrogen source, temperature, pH and light on mycelial growth and sporulation were evaluated. Among several carbon sources tested, glucose and sucrose were found superior for growth. Potassium nitrate supplemented media showed maximum growth amongst the tested inorganic nitrogen sources while peptone produced maximum growth among the tested organic nitrogen sources. Tea root extract supplemented potato dextrose agar medium was found to be the most suitable for mycelial growth and sporulation of L. theobromae. The fungus grow at temperatures ranging from 40 to 36 degrees C, with optimum growth at 28 degrees C and no growth was noted at 40 degrees C. There was no significant effect of different light period on growth of L. theobromae, but light enhanced sporulation. The fungus grow at pH 3.0-8.0 and optimum growth was observed at pH 6.0. Tea root extract supplemented potato dextrose agar medium with pH 6.0 was the most suitable for production of conidia of L. theobromae at 28 degrees C. Hence this media may be recommended for inoculum production for further studies.

  16. Missense Mutations Allow a Sequence-Blind Mutant of SpoIIIE to Successfully Translocate Chromosomes during Sporulation.

    Directory of Open Access Journals (Sweden)

    Baundauna Bose

    Full Text Available SpoIIIE directionally pumps DNA across membranes during Bacillus subtilis sporulation and vegetative growth. The sequence-reading domain (γ domain is required for directional DNA transport, and its deletion severely impairs sporulation. We selected suppressors of the spoIIIEΔγ sporulation defect. Unexpectedly, many suppressors were intragenic missense mutants, and some restore sporulation to near-wild-type levels. The mutant proteins are likely not more abundant, faster at translocating DNA, or sequence-sensitive, and rescue does not involve the SpoIIIE homolog SftA. Some mutants behave differently when co-expressed with spoIIIEΔγ, consistent with the idea that some, but not all, variants may form mixed oligomers. In full-length spoIIIE, these mutations do not affect sporulation, and yet the corresponding residues are rarely found in other SpoIIIE/FtsK family members. The suppressors do not rescue chromosome translocation defects during vegetative growth, indicating that the role of the γ domain cannot be fully replaced by these mutations. We present two models consistent with our findings: that the suppressors commit to transport in one arbitrarily-determined direction or delay spore development. It is surprising that missense mutations somehow rescue loss of an entire domain with a complex function, and this raises new questions about the mechanism by which SpoIIIE pumps DNA and the roles SpoIIIE plays in vivo.

  17. I could never take the place of your man

    DEFF Research Database (Denmark)

    Andersen, Poul Houman; Munksgaard, Kristin B.

    We explore the dynamic tensions between the strategic change intentions by business actors and the rigidities of routines in business networks. We capture strategic change acts using the recent advances in the literature on network pictures as way to understand the value creation logics of managers...... and we coin the term network picturing to describe this process. We develop a theoretical framework that links network pictures and the patterned processes of interaction in business network routines. Here, we argue that the resistance managers' meet in their change efforts is likely, even if the change...... efforts do not directly rival or contradict that of other actors in the exchange party in a resource-dependency sense, but may be caused simply by disrupting the existing business network. We use three case studies to illustrate and further explore the tensions between network picturing and network...

  18. How Does Photosynthesis Take Place in Our Oceans?

    OpenAIRE

    Ghosh, Tonmoy; Mishra, Sandhya

    2017-01-01

    The food we eat ultimately comes from plants, either directly or indirectly. The importance of plants as the global kitchen can never be underestimated. Plants “eat” sunlight and carbon dioxide to produce their own food and food for the millions of other organisms dependent on them. A molecule, chlorophyll (Chl), is crucial for this process, since it absorbs sunlight. However, the way land plants produce their food is very different from the way plants in the oceans produce their food. Since ...

  19. When radionuclides take the place of vital elements

    International Nuclear Information System (INIS)

    Rouffignac, Ch. de; Poncy, J.L.; Martin, M.

    2003-01-01

    The radiosensitivity of the kidneys is a major factor restricting the applications of chemoradiotherapy. However, improved knowledge of the mechanisms involved has allowed new treatments to be developed that attenuate the effects of the irradiation or delay its consequences. Certain radioactive elements can behave in the body like calcium, and so exhibit a special affinity for bone structures. The skin is the first tissue that is damaged by external exposure to ionising radiation. The early and late responses to irradiation in this complex organ are still poorly understood. Thanks to DNA micro-arrays, the response of skin cells to irradiation, and in particular that of epidermal stem cells, can now be studied globally. (authors)

  20. Does the measurement take place when nobody observes it

    Energy Technology Data Exchange (ETDEWEB)

    Gurvitz, Shmuel [Department of Particle Physics and Astrophysics, Weizmann Institute of Science, Rehovot (Israel); Beijing Computational Science Research Center, Beijing (China)

    2017-06-15

    We consider non-selective continuous measurements of a particle tunneling to a reservoir of finite band-width (Λ). The particle is continuously monitored by frequent projective measurements (''quantum trajectory''), separated by a time-interval τ. A simple analytical expression for the decay rate has been obtained. For Markovian reservoirs (Λ → ∞), no effect of the measurements is found. Otherwise for a finite Λ, the decay rate always depends on the measurement time τ. This result is compared with alternative calculations, with no intermediate measurements, but when the measurement device is included in the Schroedinger evolution. We found that the detector affects the system by the decoherence rate (Γ{sub d}), related to the detector's signal. Although both treatments are different, the final results become very close for τ = 2/Γ{sub d}. This τ corresponds to the minimal time for which the detector's signal can be distinguished by an ''observer''. This indicates a fundamental role of information in quantum motion and can be used for the extension of the quantum trajectory method for non-Markovian environments. (copyright 2016 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  1. BP-Mobil partnership. The common network takes place

    International Nuclear Information System (INIS)

    Anon.

    1997-01-01

    After the partnership between BP and Mobil was signed, the program of transformation of the petrol stations network started in November 1996 in the UK and concern 3300 stations in Europe and 800 stations in France. About 9100 stations will be transformed by the end of 1998. BP France is the operator for petroleum products (petrol, fuel, bitumens, LPG..) with a 70% share holding (30% for Mobil) while Mobil is the major shareholder (51%) for the lubricants and special products activities. The chemical, aviation nd maritime activities are not concerned. Thanks to the fusion of their down-file activities in Europe, the benefits of the partnership should reach 600 to 700 million of US Dollars each year. However the restructuring cost should reach 740 millions of US Dollars in two years, which doubles the initial estimation. Short paper. (J.S.)

  2. Perspective Taking in Workplaces

    Directory of Open Access Journals (Sweden)

    Zappalà Salvatore

    2014-07-01

    Full Text Available Workplaces are often described as places in which individuals are motivated by their self-interests and in which negative events like time pressure, anxiety, conflict with co-workers, miscomprehensions, difficulties in solving problems, not-transmitted or not-exchanged information that lead to mistakes, and in some cases to injuries, stress or control, are part of everyday life (Dormann & Zapf, 2002; Schabracq, Winnubst and Cooper, 2003. Such situations are often the result of the limited comprehension of needs, skills, or information available to colleagues, supervisors, subordinates, clients or providers. However, workplaces are also places in which employees take care of clients, support colleagues and subordinates (Rhoades & Eisenberger, 2002, are enthusiastic about their job (Bakker et al., 2008, are motivated by leaders that encourage employees to transcend their own self-interests for the good of the group or the organization and provide them with the confidence to perform beyond expectations (Bass, 1997. Thus positive relationships at work are becoming a new interdisciplinary domain of inquiry (Dutton & Ragins, 2006. Within this positive relationships framework, in this paper we focus on a positive component of workplaces, and particularly on an individual cognitive and emotional process that has an important role in the workplace because it facilitates interpersonal relations and communications: it is the perspective taking process. In order to describe perspective taking, we will refer to some empirical studies and particularly to the review published by Parker, Atkins and Axtell in 2008 on the International Review of Industrial and Organizational Psychology.

  3. Δ(1-pyrroline-5-carboxylate/glutamate biogenesis is required for fungal virulence and sporulation.

    Directory of Open Access Journals (Sweden)

    Ziting Yao

    Full Text Available Proline dehydrogenase (Prodh and Δ(1-pyrroline-5-carboxylate dehydrogenase (P5Cdh are two key enzymes in the cellular biogenesis of glutamate. Recombinant Prodh and P5Cdh proteins of the chestnut blight fungus Cryphonectria parasitica were investigated and showed activity in in vitro assays. Additionally, the C. parasitica Prodh and P5Cdh genes were able to complement the Saccharomyces cerevisiae put1 and put2 null mutants, respectively, to allow these proline auxotrophic yeast mutants to grow on media with proline as the sole source of nitrogen. Deletion of the Prodh gene in C. parasitica resulted in hypovirulence and a lower level of sporulation, whereas deletion of P5Cdh resulted in hypovirulence though no effect on sporulation; both Δprodh and Δp5cdh mutants were unable to grow on minimal medium with proline as the sole nitrogen source. In a wild-type strain, the intracellular level of proline and the activity of Prodh and P5Cdh increased after supplementation of exogenous proline, though the intracellular Δ(1-pyrroline-5-carboxylate (P5C content remained unchanged. Prodh and P5Cdh were both transcriptionally down-regulated in cells infected with hypovirus. The disruption of other genes with products involved in the conversion of arginine to ornithine, ornithine and glutamate to P5C, and P5C to proline in the cytosol did not appear to affect virulence; however, asexual sporulation was reduced in the Δpro1 and Δpro2 mutants. Taken together, our results showed that Prodh, P5Cdh and related mitochondrial functions are essential for virulence and that proline/glutamate pathway components may represent down-stream targets of hypovirus regulation in C. parasitica.

  4. Generation of mariner-based transposon insertion mutant library of Bacillus sphaericus 2297 and investigation of genes involved in sporulation and mosquito-larvicidal crystal protein synthesis.

    Science.gov (United States)

    Wu, Yiming; Hu, Xiaomin; Ge, Yong; Zheng, Dasheng; Yuan, Zhiming

    2012-05-01

    Bacillus sphaericus has been used with great success in mosquito control programs worldwide. Under conditions of nutrient limitation, it undergoes sporulation via a series of well defined morphological stages. However, only a small number of genes involved in sporulation have been identified. To identify genes associated with sporulation, and to understand the relationship between sporulation and crystal protein synthesis, a random mariner-based transposon insertion mutant library of B. sphaericus strain 2297 was constructed and seven sporulation-defective mutants were selected. Sequencing of the DNA flanking of the transposon insertion identified several genes involved in sporulation. The morphologies of mutants were determined by electron microscopy and synthesis of crystal proteins was analyzed by SDS-PAGE and Western blot. Four mutants blocked at early stages of sporulation failed to produce crystal proteins and had lower larvicidal activity. However, the other three mutants were blocked at later stages and were able to form crystal proteins, and the larvicidal activity was similar to wild type. These results indicated that crystal protein synthesis in B. sphaericus is dependent on sporulation initiation. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  5. Post-mortem sporulation of Ceratomyxa shasta (Myxozoa) after death in adult Chinook salmon

    Science.gov (United States)

    Kent, Michael L.; Soderlund, K.; Thomann, E.; Schreck, Carl B.; Sharpton, T.J.

    2014-01-01

    Ceratomyxa shasta (Myxozoa) is a common gastrointestinal pathogen of salmonid fishes in the Pacific Northwest of the United States. We have been investigating this parasite in adult Chinook salmon (Oncorhynchus tshawytscha) in the Willamette River, Oregon. In prior work, we observed differences in the pattern of development of C. shasta in adult salmon compared to juvenile salmon. Adult salmon consistently had large numbers of prespore stages in many of the fish that survived to spawn in the fall. However, myxospores were rarely observed, even though they were exposed and presumably infected for months before spawning. We evaluated the ability of C. shasta to sporulate following fish death because it is reported that myxosores are common in carcasses of Chinook salmon. We collected the intestine from 30 adult salmon immediately after artificial spawning and death (T0). A total of 23 fish were infected with C. shasta based on histology, but only a few myxospores were observed in 1 fish by histology. Intestines of these fish were examined at T0 and T7 (latter held at 17 C for 7 days) using quantified wet mount preparations. An increase in myxospore concentrations was seen in 39% of these fish, ranging between a 1.5- to a 14.5-fold increase. The most heavily infected fish exhibited a 4.6-fold increase from 27,841 to 129,352 myxospores/cm. This indicates, supported by various statistical analyses, that under certain conditions presporogonic forms are viable and continue to sporulate after death in adult salmon. Considering the life cycle of C. shasta and anadromous salmon, the parasite may have evolved 2, non-mutually exclusive developmental strategies. In young fish (parr and smolts), the parasite sporulates shortly after infection and is released into freshwater from either live or dead fish before their migration to seawater, where the alternate host is absent. The second strategy occurs in adult salmon, particularly spring Chinook salmon, which become infected upon

  6. Modeling curvature-dependent subcellular localization of a small sporulation protein in Bacillus subtilis

    Science.gov (United States)

    Wasnik, Vaibhav; Wingreen, Ned; Mukhopadhyay, Ranjan

    2012-02-01

    Recent experiments suggest that in the bacterium, B. subtilis, the cue for the localization of small sporulation protein, SpoVM, that plays a central role in spore coat formation, is curvature of the bacterial plasma membrane. This curvature-dependent localization is puzzling given the orders of magnitude difference in lengthscale of an individual protein and radius of curvature of the membrane. Here we develop a minimal model to study the relationship between curvature-dependent membrane absorption of SpoVM and clustering of membrane-associated SpoVM and compare our results with experiments.

  7. Comparison of the pathogenicity, growth, sporulation and morphology of Pezicula alba Guthrie (Gloeosporium album Osterw.

    Directory of Open Access Journals (Sweden)

    H. Borecka

    2013-12-01

    Full Text Available Sixteen isolates of Pezicula alba Guthr. were examined. The intensity of growth on various media, pathogenicity to apple fruits and twigs, colour of cultures and size of conidia were measured. Some isolates are pathogenic to fruits, some others to twigs; one isolate (only no. 19 is pathogenic to both twigs and fruits; many isolates are not - pathogenic at all. Culture growth, sporulation and size of conidia are not correlated with the pathogenicity of the isolate. The mean size of conidia is 21.29µm x 3.48µm.

  8. Assessment of pheromone production and response in fission yeast by a halo test of induced sporulation

    DEFF Research Database (Denmark)

    Egel, R; Willer, M; Kjaerulff, S

    1994-01-01

    We describe a rapid, sensitive and semi-quantitative plate assay for monitoring pheromone activity in the fission yeast Schizosaccharomyces pombe. It is based on the observation that meiosis requires stimulation by pheromone and exploits diploid strains that will only sporulate after addition...... of exogenous pheromone. The tester strains are heterozygous for mating type, are non-switching, and are mutated in one of the early subfunctions (either mat1-Mc or mat1-Pc), so that meiosis is only induced after exposure to exogenous pheromone (M-factor or P-factor, respectively). Pheromone activity...

  9. [Viability of nematophagous fungi Arthrobotrys robusta, Duddingtonia flagrans and Monacrosporium thaumasium after sporulation in different culture media].

    Science.gov (United States)

    Maciel, Alessandro S; de Araujo, Jackson V; Campos, Artur K

    2006-01-01

    Due to the shortage of studies that indicate the culture mediums that optimize the sporulation of namatophagous fungi for use in researche, the sporulation of the fungal isolates A. robusta (I31), D. flagrans (CG768) and M. thaumasium (NF34A) was evaluated in laboratorial conditions for 10 days in the means water-agar 2% (WA 2%), potato-dextrose-agar 2% (PDA 2%), corn-meal-agar 2% (CMA 2%) and yeast-phosphate-sulphate-sucrose-agar (YPSSA). The largest conidia production (P BDA 2% while in the isolates I31 and NF34A produced larger conidia number in YPSSA (P 0.05).

  10. Asexual sporulation signalling regulates autolysis of Aspergillus nidulans via modulating the chitinase ChiB production.

    Science.gov (United States)

    Pócsi, I; Leiter, E; Kwon, N-J; Shin, K-S; Kwon, G-S; Pusztahelyi, T; Emri, T; Abuknesha, R A; Price, R G; Yu, J-H

    2009-08-01

    Elucidation of the regulation of ChiB production in Aspergillus nidulans. Mutational inactivation of the A. nidulans chiB gene resulted in a nonautolytic phenotype. To better understand the mechanisms controlling both developmental progression and fungal autolysis, we examined a range of autolysis-associated parameters in A. nidulans developmental and/or autolytic mutants. Investigation of disorganization of mycelial pellets, loss of biomass, extra-/intracellular chitinase activities, ChiB production and chiB mRNA levels in various cultures revealed that, in submerged cultures, initialization of autolysis and stationary phase-induced ChiB production are intimately coupled, and that both processes are controlled by the FluG-BrlA asexual sporulation regulatory pathway. ChiB production does not affect the progression of apoptotic cell death in the aging A. nidulans cultures. The endochitinase ChiB plays an important role in autolysis of A. nidulans, and its production is initiated by FluG-BrlA signalling. Despite the fact that apoptosis is an inseparable part of fungal autolysis, its regulation is independent to FluG-initiated sporulation signalling. Deletion of chiB and fluG homologues in industrial filamentous fungal strains may stabilize the hyphal structures in the autolytic phase of growth and limit the release of autolytic hydrolases into the culture medium.

  11. Spore coat protein synthesis in cell-free systems from sporulating cells of Bacillus subtilis.

    Science.gov (United States)

    Nakayama, T; Munoz, L E; Sadaie, Y; Doi, R H

    1978-09-01

    Cell-free systems for protein synthesis were prepared from Bacillus subtilis 168 cells at several stages of sporulation. Immunological methods were used to determine whether spore coat protein could be synthesized in the cell-free systems prepared from sporulating cells. Spore coat protein synthesis first occurred in extracts from stage t2 cells. The proportion of spore coat protein to total proteins synthesized in the cell-free systems was 2.4 and 3.9% at stages t2 and t4, respectively. The sodium dodecyl sulfate-urea-polyacrylamide gel electrophoresis patterns of immunoprecipitates from the cell-free systems showed the complete synthesis of an apparent spore coat protein precursor (molecular weight, 25,000). A polypeptide of this weight was previously identified in studies in vivo (L.E. Munoz, Y. Sadaie, and R.H. Doi, J. Biol. Chem., in press). The synthesis in vitro of polysome-associated nascent spore coat polypeptides with varying molecular weights up to 23,000 was also detected. These results indicate that the spore coat protein may be synthesized as a precursor protein. The removal of proteases in the crude extracts by treatment with hemoglobin-Sepharose affinity techniques may be preventing the conversion of the large 25,000-dalton precursor to the 12,500-dalton mature spore coat protein.

  12. Growth and sporulation of Trichoderma polysporum on organic substrates by addition of carbon and nitrogen sources

    International Nuclear Information System (INIS)

    Rajput, A.Q.; Shahzad, S.

    2015-01-01

    During the present study nine different organic substrates viz., rice grains, sorghum grains, wheat grains, millet grains, wheat straw, rice husk, cow dung, sawdust and poultry manure were used for mass multiplication of Trichoderma polysporum. Grains, especially sorghum grains were found to be the best substrate for T. polysporum. Wheat straw and rice husk were less suitable, whereas, cow dung, sawdust and poultry manure were not suitable for growth of the fungus. Sucrose at the rate of 30,000 ppm and ammonium nitrate at the rate of 3,000 ppm were found to be the best carbon and nitrogen sources for growth and sporulation of T. polysporum. Amendment of the selected C and N sources to wheat straw, rice husk and millet grains resulted in significantly higher growth and conidia production by T. polysporum as compared to un-amended substrates. Sorghum and rice grains showed suppression in growth and sporulation of T. polysporum when amended with C and N sources. During studies on shelf life, populations of T. polysporum attained the peck at 60-135 days intervals on different substrates and declined gradually thereafter. However, even after 330 days, the populations were greater than the population at 0-day. At 345-360 days interval, populations were less than the initial populations at 0- days. Shelf life on C+N amended wheat straw and rice husk were more as compared to un-amended substrates. (author)

  13. clpC operon regulates cell architecture and sporulation in Bacillus anthracis.

    Science.gov (United States)

    Singh, Lalit K; Dhasmana, Neha; Sajid, Andaleeb; Kumar, Prasun; Bhaduri, Asani; Bharadwaj, Mitasha; Gandotra, Sheetal; Kalia, Vipin C; Das, Taposh K; Goel, Ajay K; Pomerantsev, Andrei P; Misra, Richa; Gerth, Ulf; Leppla, Stephen H; Singh, Yogendra

    2015-03-01

    The clpC operon is known to regulate several processes such as genetic competence, protein degradation and stress survival in bacteria. Here, we describe the role of clpC operon in Bacillus anthracis. We generated knockout strains of the clpC operon genes to investigate the impact of CtsR, McsA, McsB and ClpC deletion on essential processes of B. anthracis. We observed that growth, cell division, sporulation and germination were severely affected in mcsB and clpC deleted strains, while none of deletions affected toxin secretion. Growth defect in these strains was pronounced at elevated temperature. The growth pattern gets restored on complementation of mcsB and clpC in respective mutants. Electron microscopic examination revealed that mcsB and clpC deletion also causes defect in septum formation leading to cell elongation. These vegetative cell deformities were accompanied by inability of mutant strains to generate morphologically intact spores. Higher levels of polyhydroxybutyrate granules accumulation were also observed in these deletion strains, indicating a defect in sporulation process. Our results demonstrate, for the first time, the vital role played by McsB and ClpC in physiology of B. anthracis and open up further interest on this operon, which might be of importance to success of B. anthracis as pathogen. © 2014 Society for Applied Microbiology and John Wiley & Sons Ltd.

  14. Sporulation and germination gene expression analysis of Bacillus anthracis Sterne spores in skim milk under heat and different intervention techniques

    Science.gov (United States)

    To investigate how B. anthracis Stene spores survive in milk under heat (80 degree C, 10 minutes), pasteurization (72 degree C, 15 seconds) and pasteurization plus microfiltration, the expression levels of genes that related to sporulation and germination were tested using real-time PCR assays. Tw...

  15. Effect of environmental conditions and lesion age on sporulation of Phytophthora ramorum on California bay laurel, rhododendron, and camellia

    Science.gov (United States)

    Steve Tjosvold; David Chambers; Sylvia Mori

    2013-01-01

    The objective of our research was to determine the environmental conditions and lesion age favorable for Phytophthora ramorum sporulation under field conditions. For 2 years, new camellia, rhododendron, and California bay laurel (Umbellaria californica (Hook. & Arn.) Nutt.) nursery stock were seasonally inoculated (every 3 months) on foliage....

  16. Influence of glutamate on growth, sporulation, and spore properties of Bacillus cereus ATCC 14579 in defined medium

    NARCIS (Netherlands)

    Vries, de Y.P.; Atmadja, R.D.; Hornstra, L.M.; Vos, de W.M.; Abee, T.

    2005-01-01

    A chemically defined medium in combination with an airlift fermentor system was used to study the growth and sporulation of Bacillus cereus ATCC 14579. The medium contained six amino acids and lactate as the main carbon sources. The amino acids were depleted during exponential growth, while lactate

  17. Computer Vision for High-Throughput Quantitative Phenotyping: A Case Study of Grapevine Downy Mildew Sporulation and Leaf Trichomes.

    Science.gov (United States)

    Divilov, Konstantin; Wiesner-Hanks, Tyr; Barba, Paola; Cadle-Davidson, Lance; Reisch, Bruce I

    2017-12-01

    Quantitative phenotyping of downy mildew sporulation is frequently used in plant breeding and genetic studies, as well as in studies focused on pathogen biology such as chemical efficacy trials. In these scenarios, phenotyping a large number of genotypes or treatments can be advantageous but is often limited by time and cost. We present a novel computational pipeline dedicated to estimating the percent area of downy mildew sporulation from images of inoculated grapevine leaf discs in a manner that is time and cost efficient. The pipeline was tested on images from leaf disc assay experiments involving two F 1 grapevine families, one that had glabrous leaves (Vitis rupestris B38 × 'Horizon' [RH]) and another that had leaf trichomes (Horizon × V. cinerea B9 [HC]). Correlations between computer vision and manual visual ratings reached 0.89 in the RH family and 0.43 in the HC family. Additionally, we were able to use the computer vision system prior to sporulation to measure the percent leaf trichome area. We estimate that an experienced rater scoring sporulation would spend at least 90% less time using the computer vision system compared with the manual visual method. This will allow more treatments to be phenotyped in order to better understand the genetic architecture of downy mildew resistance and of leaf trichome density. We anticipate that this computer vision system will find applications in other pathosystems or traits where responses can be imaged with sufficient contrast from the background.

  18. Sporulation environment of emetic toxin-producing Bacillus cereus strains determines spore size, heat resistance and germination capacity

    NARCIS (Netherlands)

    Voort, van der M.; Abee, T.

    2013-01-01

    Aim Heat resistance, germination and outgrowth capacity of Bacillus cereus spores in processed foods are major factors in causing the emetic type of gastrointestinal disease. In this study, we aim to identify the impact of different sporulation conditions on spore properties of emetic

  19. Phosphorylation of the Bacillus subtilis Replication Controller YabA Plays a Role in Regulation of Sporulation and Biofilm Formation.

    Science.gov (United States)

    García García, Tránsito; Ventroux, Magali; Derouiche, Abderahmane; Bidnenko, Vladimir; Correia Santos, Sara; Henry, Céline; Mijakovic, Ivan; Noirot-Gros, Marie-Françoise; Poncet, Sandrine

    2018-01-01

    Bacillus subtilis cells can adopt different life-styles in response to various environmental cues, including planktonic cells during vegetative growth, sessile cells during biofilm formation and sporulation. While switching life-styles, bacteria must coordinate the progression of their cell cycle with their physiological status. Our current understanding of the regulatory pathways controlling the decision-making processes and triggering developmental switches highlights a key role of protein phosphorylation. The regulatory mechanisms that integrate the bacterial chromosome replication status with sporulation involve checkpoint proteins that target the replication initiator DnaA or the kinase phosphorelay controlling the master regulator Spo0A. B. subtilis YabA is known to interact with DnaA to prevent over-initiation of replication during vegetative growth. Here, we report that YabA is phosphorylated by YabT, a Ser/Thr kinase expressed during sporulation and biofilm formation. The phosphorylation of YabA has no effect on replication initiation control but hyper-phosphorylation of YabA leads to an increase in sporulation efficiency and a strong inhibition of biofilm formation. We also provide evidence that YabA phosphorylation affects the level of Spo0A-P in cells. These results indicate that YabA is a multifunctional protein with a dual role in regulating replication initiation and life-style switching, thereby providing a potential mechanism for cross-talk and coordination of cellular processes during adaptation to environmental change.

  20. Phosphorylation of the Bacillus subtilis Replication Controller YabA Plays a Role in Regulation of Sporulation and Biofilm Formation

    Directory of Open Access Journals (Sweden)

    Tránsito García García

    2018-03-01

    Full Text Available Bacillus subtilis cells can adopt different life-styles in response to various environmental cues, including planktonic cells during vegetative growth, sessile cells during biofilm formation and sporulation. While switching life-styles, bacteria must coordinate the progression of their cell cycle with their physiological status. Our current understanding of the regulatory pathways controlling the decision-making processes and triggering developmental switches highlights a key role of protein phosphorylation. The regulatory mechanisms that integrate the bacterial chromosome replication status with sporulation involve checkpoint proteins that target the replication initiator DnaA or the kinase phosphorelay controlling the master regulator Spo0A. B. subtilis YabA is known to interact with DnaA to prevent over-initiation of replication during vegetative growth. Here, we report that YabA is phosphorylated by YabT, a Ser/Thr kinase expressed during sporulation and biofilm formation. The phosphorylation of YabA has no effect on replication initiation control but hyper-phosphorylation of YabA leads to an increase in sporulation efficiency and a strong inhibition of biofilm formation. We also provide evidence that YabA phosphorylation affects the level of Spo0A-P in cells. These results indicate that YabA is a multifunctional protein with a dual role in regulating replication initiation and life-style switching, thereby providing a potential mechanism for cross-talk and coordination of cellular processes during adaptation to environmental change.

  1. Factors influencing the sporulation and cyst formation of Aphanomyces invadans, etiological agent of ulcerative mycosis in Atlantic menhaden, Brevoortia tyrannus

    Science.gov (United States)

    Kiryu, Y.; Blazer, V.S.; Vogelbein, W.K.; Kator, H.; Shields, J.D.

    2005-01-01

    Oomycete infections caused by Aphanomyces invadans occur in freshwater and estuarine fishes around the world. Along the east coast of the USA, skin ulcers caused by A. invadans are prevalent in Atlantic menhaden, Brevoortia tyrannus. From laboratory observations low salinities appear crucial to transmission of the pathogen. To better understand aspects of transmission, we characterized sporulation and cyst formation of secondary zoospores of two isolates of A. invadans at different salinities and temperatures. Sporulation occurred only at low salinities. At room temperature (ca. 20-22 C), using "pond water" augmented with artificial sea salts, the endemic strain WIC and the Thailand strain PA7 of A. invadans produced free-swimming secondary zoospores at salinities of 0, 1 and 2 psu (practical salinity unit = ???), but not at 4 psu or higher. Secondary zoospores of another species, ATCC-62427 (Aphanomyces sp.), were observed at 1, 2, 4 and 8 psu but not at 0 and 12 psu. Secondary zoospores of all three isolates, especially WIC, were abundant and motile 1-2 d post-sporulation. Sporulation was temperature dependent and occurred over a relatively narrow range. No sporulation occurred at 4, 30 or 35 C for either WIC or PA7. For both strains zoospore production within 1-3 d after the initiation of sporulation was more prolific at 25 C than at 20 and 15 C. At 15 C production of zoospores was sustained over 11 d for WIC and 5 d for PA7. At room temperature single WIC secondary zoospores remained motile 12-18 h. Salinities exceeding 4 psu or vigorous shaking caused immediate cyst formation of WIC secondary zoospores. Exposure to menhaden tissue, but not tissues of other fishes to secondary zoospores (WIC), caused rapid (2 h) cyst formation. Cysts were capable of excysting when transferred to 1 psu water within 2-3 h of cyst formation. Cysts that had remained encysted in 6.5 psu for 24 h did not excyst when transferred to 1 psu water. Salinity and temperature requirements

  2. Spatiotemporally regulated proteolysis to dissect the role of vegetative proteins during Bacillus subtilis sporulation: cell-specific requirement of σH and σA.

    Science.gov (United States)

    Riley, Eammon P; Trinquier, Aude; Reilly, Madeline L; Durchon, Marine; Perera, Varahenage R; Pogliano, Kit; Lopez-Garrido, Javier

    2018-04-01

    Sporulation in Bacillus subtilis is a paradigm of bacterial development, which involves the interaction between a larger mother cell and a smaller forespore. The mother cell and the forespore activate different genetic programs, leading to the production of sporulation-specific proteins. A critical gap in our understanding of sporulation is how vegetative proteins, made before sporulation initiation, contribute to spore formation. Here we present a system, spatiotemporally regulated proteolysis (STRP), which enables the rapid, developmentally regulated degradation of target proteins, thereby providing a suitable method to dissect the cell- and developmental stage-specific role of vegetative proteins. STRP has been used to dissect the role of two major vegetative sigma factors, σ H and σ A , during sporulation. The results suggest that σ H is only required in predivisional cells, where it is essential for sporulation initiation, but that it is dispensable during subsequent steps of spore formation. However, evidence has been provided that σ A plays different roles in the mother cell, where it replenishes housekeeping functions, and in the forespore, where it plays an unexpected role in promoting spore germination and outgrowth. Altogether, the results demonstrate that STRP has the potential to provide a comprehensive molecular dissection of every stage of sporulation, germination and outgrowth. © 2018 John Wiley & Sons Ltd.

  3. Triggering sporulation in Bacillus subtilis with artificial two-component systems reveals the importance of proper Spo0A activation dynamics.

    Science.gov (United States)

    Vishnoi, Monika; Narula, Jatin; Devi, Seram Nganbiton; Dao, Hoang-Anh; Igoshin, Oleg A; Fujita, Masaya

    2013-10-01

    Sporulation initiation in Bacillus subtilis is controlled by the phosphorylated form of the master regulator Spo0A which controls transcription of a multitude of sporulation genes. In this study, we investigated the importance of temporal dynamics of phosphorylated Spo0A (Spo0A∼P) accumulation by rewiring the network controlling its phosphorylation. We showed that simultaneous induction of KinC, a kinase that can directly phosphorylate Spo0A, and Spo0A itself from separately controlled inducible promoters can efficiently trigger sporulation even under nutrient rich conditions. However, the sporulation efficiency in this artificial two-component system was significantly impaired when KinC and/or Spo0A induction was too high. Using mathematical modelling, we showed that gradual accumulation of Spo0A∼P is essential for the proper temporal order of the Spo0A regulon expression, and that reduction in sporulation efficiency results from the reversal of that order. These insights led us to identify premature repression of DivIVA as one possible explanation for the adverse effects of accelerated accumulation of Spo0A∼P on sporulation. Moreover, we found that positive feedback resulting from autoregulation of the native spo0A promoter leads to robust control of Spo0A∼P accumulation kinetics. Thus we propose that a major function of the conserved architecture of the sporulation network is controlling Spo0A activation dynamics. © 2013 John Wiley & Sons Ltd.

  4. Place Branding in Systems of Place

    DEFF Research Database (Denmark)

    Zenker, Sebastian; Andéhn, Mikael

    2015-01-01

    , this presents a challenge, since the role of a place in this system of geographical abstractions constitutes a piece of information more vital than any other in defining the place. Our understanding of places cannot be separated from their scale, and any effort at managing the reputation and meaning.......g. the European Union or Africa). Using the example of nation branding for Sudan and Slovenia, one can identify supranational places such as “sub-Saharan Africa” or “Eastern Europe”, carrying their own highly salient and often negative meaning in much of the Western world. We explore how association to a system...

  5. Poly-β-hydroxybutyrate Metabolism Is Unrelated to the Sporulation and Parasporal Crystal Protein Formation in Bacillus thuringiensis.

    Science.gov (United States)

    Wang, Xun; Li, Zhou; Li, Xin; Qian, Hongliang; Cai, Xia; Li, Xinfeng; He, Jin

    2016-01-01

    Poly-3-hydroxybutyrate (PHB) is a natural polymer synthesized by many bacteria as a carbon-energy storage material. It was accumulated maximally prior to the spore formation but was degraded during the process of sporulation in Bacillus thuringiensis. Intriguingly, B. thuringiensis also accumulates large amounts of insecticidal crystal proteins (ICPs) during sporulation, which requires considerable input of carbon and energy sources. How PHB accumulation affects sporulation and ICP formation remains unclear to date. Intuitively, one would imagine that accumulated PHB provides the energy required for ICP formation. Yet our current data indicate that this is not the case. First, growth curves of the deletion mutants of phaC (encoding the PHB synthase) and phaZ (encoding the PHB depolymerase) were found to be similar to the parent strain BMB171; no difference in growth rate could be observed. In addition we further constructed the cry1Ac10 ICP gene overexpression strains of BMB171 (BMB171-cry), as well as its phaC and phaZ deletion mutants ΔphaC-cry and ΔphaZ-cry to compare their spore and ICP production rates. Again, not much change of ICP production was observed among these strains either. In fact, PHB was still degraded in most ΔphaZ-cry cells as observed by transmission electron microscopy. Together these results indicated that there is no direct association between the PHB accumulation and the sporulation and ICP formation in B. thuringiensis. Some other enzymes for PHB degradation or other energy source may be responsible for the sporulation and/or ICP formation in B. thuringiensis.

  6. A model of cell-wall dynamics during sporulation in Bacillus subtilis

    Science.gov (United States)

    Yap, Li-Wei; Endres, Robert G.

    To survive starvation, Bacillus subtilis forms durable spores. After asymmetric cell division, the septum grows around the forespore in a process called engulfment, but the mechanism of force generation is unknown. Here, we derived a novel biophysical model for the dynamics of cell-wall remodeling during engulfment based on a balancing of dissipative, active, and mechanical forces. By plotting phase diagrams, we predict that sporulation is promoted by a line tension from the attachment of the septum to the outer cell wall, as well as by an imbalance in turgor pressures in the mother-cell and forespore compartments. We also predict that significant mother-cell growth hinders engulfment. Hence, relatively simple physical principles may guide this complex biological process.

  7. Clostridium thermocellum: adhesion and sporulation while adhered to cellulose and hemicellulose

    Energy Technology Data Exchange (ETDEWEB)

    Wiegel, J.; Dykstra, M.

    1984-01-01

    During growth in the presence of fibers composed of cellulose or hemicellulose, various strains of the thermophilic soil bacterium Clostridium thermocellum and several newly isolated thermophilic anaerobic soil bacteria adhered to the fibers. Attachment occurred via a fibrous ruthenium red-staining material. C. thermocellum sporulated while attached to the fibers when the pH dropped below 6.4. It is postulated that the attachment is involved in cellulose breakdown and that C. thermocellum gaines an advantage by remaining attached to its insoluble substrates when the environment is not suitable for rapid growth. The tendency to adhere to cellulose fibers was used in the purification of thermophilic cellulolytic anaerobes. 27 references, 7 figures.

  8. The CRO-1 gene of Saccharomyces cerevisiae controls mitotic crossing over, chromosomal stability and sporulation

    International Nuclear Information System (INIS)

    Esposito, M.S.; Maleas, D.T.; Bjornstad, K.A.; Holbrook, L.L.

    1987-01-01

    The properties of a novel temperature-sensitive recombination-defective mutant of Saccharomyces cerevisiae, cro1-1 is described. The cro1-1 mutant is the first instance of a rec mutation that reduces drastically the rates of spontaneous mitotic crossing-over events but not those of gene conversional events. The cro1-1 mutation thus provides evidence that mitotic crossing-over is dependent upon gene products that are not essential for gene conversional events. The cro1-1 mutation also results in enhanced mitotic-chromosomal instability and MATa/MATα cro1-1/cro1-1 mutants are sporulation deficient. These phenotypes indicate that the CRO1 gene modulates mitotic chromosomal integrity and is essential for normal meiosis. The cro1-1 mutant possesses Holliday junction resolvase activity, hence its recombinational defect does not involve failure to execute this putative final recombinational step. 7 refs., 1 fig., 5 tabs

  9. Biocidal action of ozone-treated polystyrene surfaces on vegetative and sporulated bacteria

    International Nuclear Information System (INIS)

    Mahfoudh, Ahlem; Barbeau, Jean; Moisan, Michel; Leduc, Annie; Seguin, Jacynthe

    2010-01-01

    Surfaces of materials can be modified to ensure specific interaction features with microorganisms. The current work discloses biocidal properties of polystyrene (PS) Petri-dish surfaces that have been exposed to a dry gaseous-ozone flow. Such treated PS surfaces are able to inactivate various species of vegetative and sporulated bacteria on a relatively short contact time. Denaturation of proteins seems likely based on a significant loss of enzymatic activity of the lysozyme protein. Characterization of these surfaces by atomic-force microscopy (AFM), Fourier-transform infra-red (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS) reveals specific structural and chemical modifications as compared to untreated PS. Persistence of the biocidal properties of these treated surfaces is observed. This ozone-induced process is technically simple to achieve and does not require active precursors as in grafting.

  10. Continuing bonds and place.

    Science.gov (United States)

    Jonsson, Annika; Walter, Tony

    2017-08-01

    Where do people feel closest to those they have lost? This article explores how continuing bonds with a deceased person can be rooted in a particular place or places. Some conceptual resources are sketched, namely continuing bonds, place attachment, ancestral places, home, reminder theory, and loss of place. The authors use these concepts to analyze interview material with seven Swedes and five Britons who often thought warmly of the deceased as residing in a particular place and often performing characteristic actions. The destruction of such a place, by contrast, could create a troubling, haunting absence, complicating the deceased's absent-presence.

  11. Effects of selected herbicides and fungicides on growth, sporulation and conidial germination of entomopathogenic fungus Beauveria bassiana.

    Science.gov (United States)

    Celar, Franci A; Kos, Katarina

    2016-11-01

    The in vitro fungicidal effects of six commonly used fungicides, namely fluazinam, propineb, copper(II) hydroxide, metiram, chlorothalonil and mancozeb, and herbicides, namely isoxaflutole, fluazifop-P-butyl, flurochloridone, foramsulfuron, pendimethalin and prosulfocarb, on mycelial growth, sporulation and conidial germination of entomopathogenic fungus Beauveria bassiana (ATCC 74040) were investigated. Mycelial growth rates and sporulation at 15 and 25 °C were evaluated on PDA plates containing 100, 75, 50, 25, 12.5, 6.25 and 0% of the recommended application rate of each pesticide. The tested pesticides were classified in four scoring categories based on reduction in mycelial growth and sporulation. All pesticides, herbicides and fungicides tested had fungistatic effects of varying intensity, depending on their rate in the medium, on B. bassiana. The most inhibitory herbicides were flurochloridone and prosulfocarb, and fluazinam and copper(II) hydroxide were most inhibitory among the fungicides, while the least inhibitory were isoxaflutole and chlorothalonil. Sporulation and conidial germination of B. bassiana were significantly inhibited by all tested pesticides compared with the control treatment. Flurochloridone, foramsulfuron, prosulfocarb and copper(II) hydroxide inhibited sporulation entirely at 100% rate (99-100% inhibition), and the lowest inhibition was shown by fluazifop-P-butyl (22%) and metiram (33%). At 100% dosage, all herbicides in the test showed a high inhibitory effect on conidial germination. Conidial germination inhibition ranged from 82% with isoxaflutole to 100% with fluorochloridone, pendimethalin and prosulfocarb. At 200% dosage, inhibition rates even increased (96-100%). All 12 pesticides tested had a fungistatic effect on B. bassiana of varying intensity, depending on the pesticide and its concentration. B. bassiana is highly affected by some herbicides and fungicides even at very low rates. Flurochloridone, foramsulfuron

  12. Dynamic sporulation gene co-expression networks for Bacillus subtilis 168 and the food-borne isolate Bacillus amyloliquefaciens: a transcriptomic model.

    Science.gov (United States)

    Omony, Jimmy; de Jong, Anne; Krawczyk, Antonina O; Eijlander, Robyn T; Kuipers, Oscar P

    2018-02-09

    Sporulation is a survival strategy, adapted by bacterial cells in response to harsh environmental adversities. The adaptation potential differs between strains and the variations may arise from differences in gene regulation. Gene networks are a valuable way of studying such regulation processes and establishing associations between genes. We reconstructed and compared sporulation gene co-expression networks (GCNs) of the model laboratory strain Bacillus subtilis 168 and the food-borne industrial isolate Bacillus amyloliquefaciens. Transcriptome data obtained from samples of six stages during the sporulation process were used for network inference. Subsequently, a gene set enrichment analysis was performed to compare the reconstructed GCNs of B. subtilis 168 and B. amyloliquefaciens with respect to biological functions, which showed the enriched modules with coherent functional groups associated with sporulation. On basis of the GCNs and time-evolution of differentially expressed genes, we could identify novel candidate genes strongly associated with sporulation in B. subtilis 168 and B. amyloliquefaciens. The GCNs offer a framework for exploring transcription factors, their targets, and co-expressed genes during sporulation. Furthermore, the methodology described here can conveniently be applied to other species or biological processes.

  13. Salt-sensitivity of σ(H) and Spo0A prevents sporulation of Bacillus subtilis at high osmolarity avoiding death during cellular differentiation.

    Science.gov (United States)

    Widderich, Nils; Rodrigues, Christopher D A; Commichau, Fabian M; Fischer, Kathleen E; Ramirez-Guadiana, Fernando H; Rudner, David Z; Bremer, Erhard

    2016-04-01

    The spore-forming bacterium Bacillus subtilis frequently experiences high osmolarity as a result of desiccation in the soil. The formation of a highly desiccation-resistant endospore might serve as a logical osmostress escape route when vegetative growth is no longer possible. However, sporulation efficiency drastically decreases concomitant with an increase in the external salinity. Fluorescence microscopy of sporulation-specific promoter fusions to gfp revealed that high salinity blocks entry into the sporulation pathway at a very early stage. Specifically, we show that both Spo0A- and SigH-dependent transcription are impaired. Furthermore, we demonstrate that the association of SigH with core RNA polymerase is reduced under these conditions. Suppressors that modestly increase sporulation efficiency at high salinity map to the coding region of sigH and in the regulatory region of kinA, encoding one the sensor kinases that activates Spo0A. These findings led us to discover that B. subtilis cells that overproduce KinA can bypass the salt-imposed block in sporulation. Importantly, these cells are impaired in the morphological process of engulfment and late forespore gene expression and frequently undergo lysis. Altogether our data indicate that B. subtilis blocks entry into sporulation in high-salinity environments preventing commitment to a developmental program that it cannot complete. © 2015 John Wiley & Sons Ltd.

  14. Salt-sensitivity of σH and Spo0A prevents sporulation of Bacillus subtilis at high osmolarity avoiding death during cellular differentiation

    Science.gov (United States)

    Widderich, Nils; Rodrigues, Christopher D.A.; Commichau, Fabian M.; Fischer, Kathleen E.; Ramirez-Guadiana, Fernando H.; Rudner, David Z.; Bremer, Erhard

    2016-01-01

    Summary The spore-forming bacterium Bacillus subtilis frequently experiences high osmolarity as a result of desiccation in the soil. The formation of a highly desiccation-resistant endospore might serve as a logical osmostress escape route when vegetative growth is no longer possible. However, sporulation efficiency drastically decreases concomitant with an increase in the external salinity. Fluorescence microscopy of sporulation-specific promoter fusions to gfp revealed that high salinity blocks entry into the sporulation pathway at a very early stage. Specifically, we show that both Spo0A- and SigH-dependent transcription are impaired. Furthermore, we demonstrate that the association of SigH with core RNA polymerase is reduced under these conditions. Suppressors that modestly increase sporulation efficiency at high salinity map to the coding region of sigH and in the regulatory region of kinA, encoding one the sensor kinases that activates Spo0A. These findings led us to discover that B. subtilis cells that overproduce KinA can bypass the salt-imposed block in sporulation. Importantly, these cells are impaired in the morphological process of engulfment and late forespore gene expression and frequently undergo lysis. Altogether our data indicate that B. subtilis blocks entry into sporulation in high-salinity environments preventing commitment to a developmental program that it cannot complete. PMID:26712348

  15. My Place Is Not Your Place

    DEFF Research Database (Denmark)

    Zenker, Sebastian; Beckmann, Suzanne C.

    2013-01-01

    Purpose – Cities increasingly compete with each other for attracting tourists, investors, companies, or residents. Marketers therefore focus on establishing the city as a brand, disregarding that the perception and knowledge of a city differ dramatically between the target audiences. Hence, place...... branding should emphasize much more the perceptions of the different target groups and develop strategies for advanced place brand management. The aim of this paper is to assess the important discrepancies between the city brand perceptions of different target groups with the help of network analysis......-ended-question survey with 334 participants. Findings – Structural differences for the city brand perceptions of two different target groups and the differences between perceptions of an external and internal target group are highlighted. The results and the managerial implications for place marketers are discussed...

  16. Evidence that Autophosphorylation of the Major Sporulation Kinase in Bacillus subtilis Is Able To Occur in trans.

    Science.gov (United States)

    Devi, Seram Nganbiton; Kiehler, Brittany; Haggett, Lindsey; Fujita, Masaya

    2015-08-01

    Entry into sporulation in Bacillus subtilis is governed by a multicomponent phosphorelay, a complex version of a two-component system which includes at least three histidine kinases (KinA to KinC), two phosphotransferases (Spo0F and Spo0B), and a response regulator (Spo0A). Among the three histidine kinases, KinA is known as the major sporulation kinase; it is autophosphorylated with ATP upon starvation and then transfers a phosphoryl group to the downstream components in a His-Asp-His-Asp signaling pathway. Our recent study demonstrated that KinA forms a homotetramer, not a dimer, mediated by the N-terminal domain, as a functional unit. Furthermore, when the N-terminal domain was overexpressed in the starving wild-type strain, sporulation was impaired. We hypothesized that this impairment of sporulation could be explained by the formation of a nonfunctional heterotetramer of KinA, resulting in the reduced level of phosphorylated Spo0A (Spo0A∼P), and thus, autophosphorylation of KinA could occur in trans. To test this hypothesis, we generated a series of B. subtilis strains expressing homo- or heterogeneous KinA protein complexes consisting of various combinations of the phosphoryl-accepting histidine point mutant protein and the catalytic ATP-binding domain point mutant protein. We found that the ATP-binding-deficient protein was phosphorylated when the phosphorylation-deficient protein was present in a 1:1 stoichiometry in the tetramer complex, while each of the mutant homocomplexes was not phosphorylated. These results suggest that ATP initially binds to one protomer within the tetramer complex and then the γ-phosphoryl group is transmitted to another in a trans fashion. We further found that the sporulation defect of each of the mutant proteins is complemented when the proteins are coexpressed in vivo. Taken together, these in vitro and in vivo results reinforce the evidence that KinA autophosphorylation is able to occur in a trans fashion

  17. Place branding, embeddedness and endogenous rural development

    NARCIS (Netherlands)

    Donner, Mechthild; Horlings, Lummina; Fort, Fatiha; Vellema, Sietze

    2017-01-01

    This article deals with place branding on the regional scale, in the rural context of food and tourism networks in Europe. Place branding is linked to the concepts of endogenous rural development, territory and embeddedness, by analysing how the valorisation of specific rural assets takes shape.

  18. Populated Places of Iowa

    Data.gov (United States)

    Iowa State University GIS Support and Research Facility — This coverage contains points that represent populated places, ie. cities, towns, villages or any other named place where people live. The coverage was developed...

  19. Les bactéries sporulées dans les conserves de légumes (petits pois ...

    African Journals Online (AJOL)

    Unicornis

    brevis (4.53%). Mots clés : Conserves végétales - Petits pois- Bactéries sporulées - Identification biochimique. Abstract. For a consumer more and more aware about the risks for his health, regarding the food he buys, the aim of this study is to verify the hygienic quality of ten batches, each one contains five green peas cans, ...

  20. The importance of places and place branding

    NARCIS (Netherlands)

    Kovács, Z.; Musterd, S.; Musterd, S.; Kovács, Z.

    2013-01-01

    In the analytical part of this study, we highlight that, despite globalisation and growing uniformity, there is still an important role for place itself in the location decisions of economic players. Part III of this volume deals with this. Even though city-regions across the world have become

  1. Effect of iodoacetic acid on 59Fe uptake and aconitase aivity during sporulation of Bacillus cereus T

    International Nuclear Information System (INIS)

    Twari, B.K.; Sharma, D.

    1975-01-01

    Iodoacetic acid (IAA), a well known inhibitor of glycolysis, inhibited sporulation of B. cereus T when added to the culture just prior to the transition stage at 2-2.5 hr. In the inhibited culture, no considerable aconitase activity and 59 Fe uptake were observed. Time studies with IAA in modified G-medium had shown that whenever it was added it prevented further glycolysis of glucose. Addition of IAA at zero hr had no effect on aconitase activity and 59 Fe uptake whether glucose was present or absent from the medium. IAA added at rising pH at 3 hr. i.e. after transition period had no effect on the pH characteristics and sporulation of the organism. IAA seems to inhibit the induction of metal transport system. There exists a considerable correlation between aconitase activity and 59 Fe uptake during growth and sporulation of B. cereus T in modified G-medium in the presence and absence of glucose. (author)

  2. Impacts of sporulation temperature, exposure to compost matrix and temperature on survival of Bacillus cereus spores during livestock mortality composting.

    Science.gov (United States)

    Stanford, K; Reuter, T; Gilroyed, B H; McAllister, T A

    2015-04-01

    To investigate impact of sporulation and compost temperatures on feasibility of composting for disposal of carcasses contaminated with Bacillus anthracis. Two strains of B. cereus, 805 and 1391, were sporulated at either 20 or 37°C (Sporulation temperature, ST) and 7 Log10 CFU g(-1) spores added to autoclaved manure in nylon bags (pore size 50 μm) or in sealed vials. Vials and nylon bags were embedded into compost in either a sawdust or manure matrix each containing 16 bovine mortalities (average weight 617 ± 33 kg), retrieved from compost at intervals over 217 days and survival of B. cereus spores assessed. A ST of 20°C decreased spore survival by 1·4 log10 CFU g(-1) (P Compost temperatures >55°C reduced spore survival (P compost temperatures were key factors influencing survival of B. cereus spores in mortality compost. Composting may be most appropriate for the disposal of carcasses infected with B. anthracis at ambient temperatures ≤20°C under thermophillic composting conditions (>55°C). © 2015 The Society for Applied Microbiology.

  3. A woman's rightful place?

    Science.gov (United States)

    1993-04-01

    Rural development projects in sub-Saharan Africa tend not to succeed because they do not consider women's role and their significance, even though women constitute 70% of agricultural workers, 80% of food producers, 100% of people who prepare meals, and 60-90% do food marketing. Development specialists ignore women because they are not involved in political activities and in decision making. As long as women and women's contributions are not considered, rural development projects will remain inefficient and development will not take place. Thus, projects must include women as agents and beneficiaries of development in key sectors of the economy. Rural development specialists must also consider the effect male labor emigration has on rural women. For example, drought has forced many men to leave their villages, leaving a work force consisting of 95% women to fight desertification. All too often, women have no or limited land ownership rights, thereby keeping them from improving the land, e.g., planting perennial fruit crops. They also tend to be hired hands rather than food producers. They cannot obtain bank loans because they do not own land, and because they are often illiterate (over 90% female illiteracy in 28 African countries), they can neither understand nor complete bank loan forms. Rural development projects further alienate women by aiming training programs to men or by using male agricultural extension agents. Women react to this alienation by rejecting projects that do not benefit them and follow more profitable activities which sometimes interfere with projects. Thus, rural development programs need to invest in women to ensure viable and efficient sustainable development.

  4. The Influence of Sporulation Conditions on the Spore Coat Protein Composition of Bacillus subtilis Spores.

    Science.gov (United States)

    Abhyankar, Wishwas R; Kamphorst, Kiki; Swarge, Bhagyashree N; van Veen, Henk; van der Wel, Nicole N; Brul, Stanley; de Koster, Chris G; de Koning, Leo J

    2016-01-01

    Spores are of high interest to the food and health sectors because of their extreme resistance to harsh conditions, especially against heat. Earlier research has shown that spores prepared on solid agar plates have a higher heat resistance than those prepared under a liquid medium condition. It has also been shown that the more mature a spore is, the higher is its heat resistance most likely mediated, at least in part, by the progressive cross-linking of coat proteins. The current study for the first time assesses, at the proteomic level, the effect of two commonly used sporulation conditions on spore protein presence. 14 N spores prepared on solid Schaeffer's-glucose (SG) agar plates and 15 N metabolically labeled spores prepared in shake flasks containing 3-( N -morpholino) propane sulfonic acid (MOPS) buffered defined liquid medium differ in their coat protein composition as revealed by LC-FT-MS/MS analyses. The former condition mimics the industrial settings while the latter conditions mimic the routine laboratory environment wherein spores are developed. As seen previously in many studies, the spores prepared on the solid agar plates show a higher thermal resistance than the spores prepared under liquid culture conditions. The 14 N: 15 N isotopic ratio of the 1:1 mixture of the spore suspensions exposes that most of the identified inner coat and crust proteins are significantly more abundant while most of the outer coat proteins are significantly less abundant for the spores prepared on solid SG agar plates relative to the spores prepared in the liquid MOPS buffered defined medium. Sporulation condition-specific differences and variation in isotopic ratios between the tryptic peptides of expected cross-linked proteins suggest that the coat protein cross-linking may also be condition specific. Since the core dipicolinic acid content is found to be similar in both the spore populations, it appears that the difference in wet heat resistance is connected to the

  5. The Influence of Sporulation Conditions on the Spore Coat Protein Composition of Bacillus subtilis Spores

    Science.gov (United States)

    Abhyankar, Wishwas R.; Kamphorst, Kiki; Swarge, Bhagyashree N.; van Veen, Henk; van der Wel, Nicole N.; Brul, Stanley; de Koster, Chris G.; de Koning, Leo J.

    2016-01-01

    Spores are of high interest to the food and health sectors because of their extreme resistance to harsh conditions, especially against heat. Earlier research has shown that spores prepared on solid agar plates have a higher heat resistance than those prepared under a liquid medium condition. It has also been shown that the more mature a spore is, the higher is its heat resistance most likely mediated, at least in part, by the progressive cross-linking of coat proteins. The current study for the first time assesses, at the proteomic level, the effect of two commonly used sporulation conditions on spore protein presence. 14N spores prepared on solid Schaeffer’s-glucose (SG) agar plates and 15N metabolically labeled spores prepared in shake flasks containing 3-(N-morpholino) propane sulfonic acid (MOPS) buffered defined liquid medium differ in their coat protein composition as revealed by LC-FT-MS/MS analyses. The former condition mimics the industrial settings while the latter conditions mimic the routine laboratory environment wherein spores are developed. As seen previously in many studies, the spores prepared on the solid agar plates show a higher thermal resistance than the spores prepared under liquid culture conditions. The 14N:15N isotopic ratio of the 1:1 mixture of the spore suspensions exposes that most of the identified inner coat and crust proteins are significantly more abundant while most of the outer coat proteins are significantly less abundant for the spores prepared on solid SG agar plates relative to the spores prepared in the liquid MOPS buffered defined medium. Sporulation condition-specific differences and variation in isotopic ratios between the tryptic peptides of expected cross-linked proteins suggest that the coat protein cross-linking may also be condition specific. Since the core dipicolinic acid content is found to be similar in both the spore populations, it appears that the difference in wet heat resistance is connected to the

  6. The influence of sporulation conditions on the spore coat protein composition of Bacillus subtilis spores.

    Directory of Open Access Journals (Sweden)

    Wishwas R. Abhyankar

    2016-10-01

    Full Text Available Spores are of high interest to the food and health sectors because of their extreme resistance to harsh conditions, especially against heat. Earlier research has shown that spores prepared on solid agar plates have a higher heat resistance than those prepared under a liquid medium condition. It has also been shown that the more mature a spore is, the higher is its heat resistance most likely mediated, at least in part, by the progressive cross-linking of coat proteins. The current study for the first time assesses, at the proteomic level, the effect of two commonly used sporulation conditions on spore protein presence. 14N spores prepared on solid SG agar plates and 15N metabolically labelled spores prepared in shake flasks containing MOPS buffered defined liquid medium differ in their coat protein composition as revealed by LC-FT-MS/MS analyses. The former condition mimics the industrial settings while the latter conditions mimic the routine laboratory environment wherein spores are developed. As seen previously in many studies, the spores prepared on the solid agar plates show a higher thermal resistance than the spores prepared under liquid culture conditions. The 14N: 15N isotopic ratio of the 1:1 mixture of the spore suspensions exposes that most of the identified inner coat and crust proteins are significantly more abundant while most of the outer coat proteins are significantly less abundant for the spores prepared on solid SG agar plates relative to the spores prepared in the liquid MOPS buffered defined medium. Sporulation condition-specific differences and variation in isotopic ratios between the tryptic peptides of expected cross-linked proteins suggest that the coat protein cross-linking may also be condition specific. Since the core dipicolinic acid content is found to be similar in both the spore populations, it appears that the difference in wet heat resistance is connected to the differences in the coat protein composition and

  7. Taking warfarin (Coumadin)

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/patientinstructions/000292.htm Taking warfarin (Coumadin) To use the sharing features on this ... form a clot or have bleeding problems. Taking Warfarin It is important that you take warfarin exactly ...

  8. Take Charge. Take the Test. PSA (:30)

    Centers for Disease Control (CDC) Podcasts

    As part of the Take Charge. Take the Test. campaign, this 30 second PSA encourages African American women to get tested for HIV. Locations for a free HIV test can be found by visiting hivtest.org/takecharge or calling 1-800-CDC-INFO (1-800-232-4636).

  9. Place in Transition

    DEFF Research Database (Denmark)

    Mikkelsen, Jacob Bjerre; Lange, Ida Sofie Gøtzsche

    from the 'Everyday World'. Within mobilities studies, research has focused on different aspects and consequences of the post-oil society (see Dennis & Urry 2009, Urry 2013). This paper discusses the conception of place within the enclosed 'Oil World' with point of departure in relocation...... and redefinition of oil rigs from an urban design perspective. The paper constitutes a theoretical basis for future design scenarios - exemplified through visionary urban design proposals for a specific site in the city of Esbjerg, Denmark. Relocating rigs to an urban context initiates discussions of conception...... of 'Place' questioning the fixity of 'Place' (Jensen 2010). Scoped through a relational sense of place (Massey 1993) and the potential of exploring new relations between places (Burns & Kahn 2005), the paper challenges the notion of 'Place as God' (Hvattum 2010). These places in transition contest...

  10. Taking climate to the market

    International Nuclear Information System (INIS)

    Boyle, S.

    1998-01-01

    Since the 1997 Kyoto Protocol set binding targets for greenhouse gas reductions, there has been a rapid increase in interest in emissions trading. This is based on the premise that reduction options may be cheaper in countries such as those of eastern Europe and the developing world, than in others such as the USA and Norway. As long as real emission reductions take place, and given that climate change is a global problem, setting up an emissions trading market should provide a much cheaper option than reductions applied solely at a national level. The real prospects for a viable market, what it will look like, and the deals already taking place are examined. (UK)

  11. Place-Specific Computing

    DEFF Research Database (Denmark)

    Messeter, Jörn

    2009-01-01

    An increased interest in the notion of place has evolved in interaction design based on the proliferation of wireless infrastructures, developments in digital media, and a ‘spatial turn’ in computing. In this article, place-specific computing is suggested as a genre of interaction design that add......An increased interest in the notion of place has evolved in interaction design based on the proliferation of wireless infrastructures, developments in digital media, and a ‘spatial turn’ in computing. In this article, place-specific computing is suggested as a genre of interaction design...... that addresses the shaping of interactions among people, place-specific resources and global socio-technical networks, mediated by digital technology, and influenced by the structuring conditions of place. The theoretical grounding for place-specific computing is located in the meeting between conceptions...... of place in human geography and recent research in interaction design focusing on embodied interaction. Central themes in this grounding revolve around place and its relation to embodiment and practice, as well as the social, cultural and material aspects conditioning the enactment of place. Selected...

  12. MrSkn7 controls sporulation, cell wall integrity, autolysis, and virulence in Metarhizium robertsii.

    Science.gov (United States)

    Shang, Yanfang; Chen, Peilin; Chen, Yixiong; Lu, Yuzhen; Wang, Chengshu

    2015-04-01

    Two-component signaling pathways generally include sensor histidine kinases and response regulators. We identified an ortholog of the response regulator protein Skn7 in the insect-pathogenic fungus Metarhizium robertsii, which we named MrSkn7. Gene deletion assays and functional characterizations indicated that MrSkn7 functions as a transcription factor. The MrSkn7 null mutant of M. robertsii lost the ability to sporulate and had defects in cell wall biosynthesis but was not sensitive to oxidative and osmotic stresses compared to the wild type. However, the mutant was able to produce spores under salt stress. Insect bioassays using these spores showed that the virulence of the mutant was significantly impaired compared to that of the wild type due to the failures to form the infection structure appressorium and evade host immunity. In particular, deletion of MrSkn7 triggered cell autolysis with typical features such as cell vacuolization, downregulation of repressor genes, and upregulation of autolysis-related genes such as extracellular chitinases and proteases. Promoter binding assays confirmed that MrSkn7 could directly or indirectly control different putative target genes. Taken together, the results of this study help us understand the functional divergence of Skn7 orthologs as well as the mechanisms underlying the development and control of virulence in insect-pathogenic fungi. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  13. Experimental Infection with Sporulated Oocysts of Eimeria maxima (Apicomplexa: Eimeriidae) in Broiler

    Science.gov (United States)

    Brito, Luciana da S.; Pereira, Elder N.; da Silva, Augusta A.; Bentivóglio Costa Silva, Vinícius; Freitas, Fagner L. da C.

    2014-01-01

    Through this study we assessed the metabolic and pathological changes in broilers experimentally infected with oocysts of Eimeria maxima. To perform the experiment, we used 150 broiler strain cooB males, with ten days of age, were randomized according to weight and randomly assigned to two experimental groups: the control group was inoculated with 0.5 mL of distilled water; the infected group inoculated with 0.5 mL of solution containing 5 × 104 sporulated oocysts of Eimeria maxima. The live performance was evaluated on day 0 (day of inoculation), 5°, 10°, 15°, 25°, and 35° dpi, being slaughtered by cervical dislocation, fifteen birds/group. Although the sum in meat production was higher in the control group, the weight of the heart and gizzard of the experimental animals showed no significant difference, while the liver had difference on day 5°, 15°, and 35° dpi. The pathologic evaluation showed congested mucosa and presence of large amounts of mucus at 6 dpi. Therefore, it is concluded that the dose of 5 × 104  E. maxima inoculated in the experimental group was enough to cause harm to the animal organism. PMID:26464925

  14. Metabolic alterations in broiler chickens experimentally infected with sporulated oocysts of Eimeria maxima.

    Science.gov (United States)

    Freitas, Fagner Luiz da Costa

    2014-01-01

    Metabolic and morphometric alterations of the duodenal villi caused by parasitism of chickens by Eimeria maxima were evaluated, using 100 male Cobb birds, randomly distributed into two groups (control and infected). The infected group was inoculated with 0.5 ml of a solution containing 5 × 10³ sporulated oocysts of Eimeria maxima. Ten birds per sample were sacrificed on the 6th, 11th, 22nd and 41st days post-infection (dpi). In order to evaluate the alterations, samples of duodenum, jejunum and ileum fragments were collected after necropsy for histological analysis. Villus biometry was determined by means of a slide graduated in microns that was attached to a binocular microscope. To evaluate the biochemical data, 5 ml of blood were sampled from the birds before sacrifice. The statistical analyses were performed using the GraphPad 5 statistical software for Windows. Tukey's multiple comparison test (p maxima causes both qualitative and quantitative alterations to the structure of the intestinal villi, thereby interfering with the absorption of nutrients such as calcium, phosphorus, magnesium, protein and lipids, with consequent reductions in the birds' weights.

  15. From sense of place to visualization of place: examining people-place relationships for insight on developing geovisualizations.

    Science.gov (United States)

    Newell, Robert; Canessa, Rosaline

    2018-02-01

    Effective resource planning incorporates people-place relationships, allowing these efforts to be inclusive of the different local beliefs, interests, activities and needs. 'Geovisualizations' can serve as potentially powerful tools for facilitating 'place-conscious' resource planning, as they can be developed with high degrees of realism and accuracy, allowing people to recognize and relate to them as 'real places'. However, little research has been done on this potential, and the place-based applications of these visual tools are poorly understood. This study takes steps toward addressing this gap by exploring the relationship between sense of place and 'visualization of place'. Residents of the Capital Regional District of BC, Canada, were surveyed about their relationship with local coastal places, concerns for the coast, and how they mentally visualize these places. Factor analysis identified four sense of place dimensions - nature protection values, community and economic well-being values, place identity and place dependence, and four coastal concerns dimensions - ecological, private opportunities, public space and boating impacts. Visualization data were coded and treated as dependent variables in a series of logistic regressions that used sense of place and coastal concerns dimensions as predictors. Results indicated that different aspects of sense of place and (to a lesser degree) concerns for places influence the types of elements people include in their mental visualization of place. In addition, sense of place influenced the position and perspective people assume in these visualizations. These findings suggest that key visual elements and perspectives speak to different place relationships, which has implications for developing and using geovisualizations in terms of what elements should be included in tools and (if appropriate) depicted as affected by potential management or development scenarios.

  16. Comparison of expression of key sporulation, solventogenic and acetogenic genes in C. beijerinckii NRRL B-598 and its mutant strain overexpressing spo0A.

    Science.gov (United States)

    Kolek, J; Diallo, M; Vasylkivska, M; Branska, B; Sedlar, K; López-Contreras, A M; Patakova, P

    2017-11-01

    The production of acetone, butanol and ethanol by fermentation of renewable biomass has potential to become a valuable industrial process. Mechanisms of solvent production and sporulation involve some common regulators in some ABE-producing clostridia, although details of the links between the pathways are not clear. In this study, we compare a wild-type (WT) Clostridium beijerinckii NRRL B-598 with its mutant strain OESpo0A, in which the gene encoding Spo0A, an important regulator of both sporulation and solventogenesis, is overexpressed in terms of solvent and acid production. We also compare morphologies during growth on two different media: TYA broth, where the WT culture sporulates, and RCM, where the WT culture does not. In addition, RT-qPCR-based analysis of expression profiles of spo0A, spoIIE, sigG, spoVD, ald and buk1 genes involved in sporulation or solvent production in these strains, were compared. The OESpo0A mutant did not produce spores and butanol titre was lower compared to the WT, but increased amounts of butyric acid and ethanol were produced. The gene spo0A had high levels of expression in the WT under non-sporulating culture conditions while other selected genes for sporulation factors were downregulated significantly. Similar observations were obtained for OESpo0A where spo0A overexpression and downregulation of other sporulation genes were demonstrated. Higher expression of spo0A led to higher expression of buk1 and ald, which could confirm the role of spo0A in activation of the solventogenic pathway, although solvent production was not affected significantly in the WT and was weakened in the OESpo0A mutant.

  17. Management and communication courses – Places available

    CERN Multimedia

    2012-01-01

    There are places available in some management and communication courses taking place in the period January to March 2013.   For more information on the course, click on the course title, which will bring you to the training catalogue. You can then sign-up on-line. For advice, you can contact: Erwin Mosselmans, tel. 74125, erwin.mosselmans@cern.ch Nathalie Dumeaux, tel. 78144, nathalie.dumeaux@cern.ch Courses in English (or bilingual) Session Duration Language Availability How to get, as a supervisor, the most out of the annual interview 21 January 1 day English 2 places Project Engineering 24 and 25 January 2 days English 6 places How to get, as a supervisor, the most out of the annual interview 31 January 1 day English 6 places Conflict Resolution for Managers 19 and 20 February 2 days English One more place Project Scheduling & Costing 6 and 7 March 2 days English 2 places Communicati...

  18. Take Charge. Take the Test. PSA (:30)

    Centers for Disease Control (CDC) Podcasts

    2012-03-07

    As part of the Take Charge. Take the Test. campaign, this 30 second PSA encourages African American women to get tested for HIV. Locations for a free HIV test can be found by visiting hivtest.org/takecharge or calling 1-800-CDC-INFO (1-800-232-4636).  Created: 3/7/2012 by National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention (NCHHSTP).   Date Released: 3/7/2012.

  19. The value of place

    Science.gov (United States)

    Dentzau, Michael W.

    2014-03-01

    This commentary seeks to expand the dialogue on place-based science education presented in Katie Lynn Brkich's article, where the connections fifth grade students make between their formal earth science curriculum and their lived experiences are highlighted. The disconnect between the curriculum the students are offered and their immediate environment is clear, and we are presented with examples of how they strive to make connections between the content and what they are familiar with—namely their surroundings. "Place" is identified as a term with complex meanings and interpretations, even in the scope of place-based science education, and understanding how the term is used in any given scenario is essential to understanding the implications of place-based education. Is place used as a location, locale or a sense of place? To understand "place" is to acknowledge that for the individual, it is highly situational, cultural and personal. It is just such attributes that make place-based education appealing, and potentially powerful, pedagogically on one hand, yet complex for implementation on the other. The argument is posed that place is particularly important in the context of education about the environment, which in its simplest manifestation, connects formal science curriculum to resources that are local and tangible to students. The incorporation of place in such a framework seeks to bridge the gap between formal school science subjects and students' lived experiences, yet acknowledges the tensions that can arise between accommodating place meanings and the desire to acculturate students into the language of the scientific community. The disconnect between guiding policy frameworks and the reality of the Next Generation Science Standards is addressed opening an avenue for further discussion of the importance of socio-cultural frameworks of science learning in an ever increasing era of accountability.

  20. Technical training: places available

    CERN Multimedia

    HR Department

    2007-01-01

    CERN Technical Training: Open Courses (April - June 2007) The following course sessions are currently scheduled in the framework of the CERN Technical Training Programme 2007:   AutoCAD 2006 - niveau 1 (course in French): 25.4.- 26.4.2007 & 2.5. - 3.5.2007 (4 days in 2 modules, 5 places available) AutoCAD 2006 - niveau 1 (course in French): 27.6.- 28.6.2007 & 3.7. - 4.7.2007 (4 days in 2 modules, 5 places available) AutoCAD Mechanical 2006 (course in French) 21.6.-22.6.2007 (2 days, 8 places available) * NEW COURSE* Automate de securite S7 (course in French) 14.5.-16.5.2007 (3 days, 4 places available) * NEW COURSE* Automate de securite S7 (course in French): 9.5.-11.5.2007 (3 days, 4 places available) JCOP - Joint PVSS-JCOP Frameswork (course in English): 21.5.-25.5.2007 (5 days, 12 places available) JCOP - Finite State Machines in the JCOP Frameswork (course in English): 12.6.-14.6.2007 (3 days, 12 places available) LabVIEW Basics 1 (in English): 2.-4.5.2007 (3 days, 7 places ...

  1. A Sense of Place

    Directory of Open Access Journals (Sweden)

    Rachel Black

    2012-09-01

    Full Text Available People increasingly want to know where their food and wine comes from and who produces it. This is part of developing a taste of place, or what the French call terroir. The academic and industry debates surrounding the concept of terroir are explored, and the efforts of Massachusetts wine producers to define their sense of place are discussed.

  2. Place in transition

    DEFF Research Database (Denmark)

    Mikkelsen, Jacob Bjerre; Lange, Ida Sofie Gøtzsche

    2017-01-01

    World. This paper discusses the conception of place in the Oil World, with the relocation and transformation of oil rigs from an urban design perspective as its point of departure, using Esbjerg, Denmark, as a case study. Combining a theoretical understanding of places as relational with a design...

  3. The Case for Place

    Science.gov (United States)

    Thomas, Lisa Carlucci

    2012-01-01

    Bookstores, record stores, libraries, Facebook: these places--both physical and virtual--demonstrate an established and essential purpose as centers of community, expertise, convenience, immediacy, and respect. Yet as digital, mobile, and social shifts continue to transform culture and interactions, these spaces and places transform, too.…

  4. Non-Place

    DEFF Research Database (Denmark)

    formulation. The anthology contains 17 articles engaged directly in the application, retrofitting and broadening of the concept of the non-place to a range of literary and media texts, as well as the merging of this concept to other theoretical concepts by e.g. Michel Foucault, Gilles Deleuze, Alain Badiou......We spend more and more of our everyday lives in what Marc Augé calls non-places – homogenous, but bland places of transit. This anthology addresses the representations of non-places in literature, culture and media, and critiques and re-actualizes Augé’s work twenty years after its initial...... Jutland, and many others. This anthology is the seventh publication in the IRGiC series and it springs from a research seminar held at Aalborg University in May 2013: “Non-Place in Literature, Media and Culture”....

  5. Place-Specific Computing

    DEFF Research Database (Denmark)

    Messeter, Jörn; Johansson, Michael

    project place- specific computing is explored through design oriented research. This article reports six pilot studies where design students have designed concepts for place-specific computing in Berlin (Germany), Cape Town (South Africa), Rome (Italy) and Malmö (Sweden). Background and arguments...... for place-specific computing as a genre of interaction design are described. A total number of 36 design concepts designed for 16 designated zones in the four cities are presented. An analysis of the design concepts is presented indicating potentials, possibilities and problems as directions for future......An increased interest in the notion of place has evolved in interaction design. Proliferation of wireless infrastructure, developments in digital media, and a ‘spatial turn’ in computing provides the base for place-specific computing as a suggested new genre of interaction design. In the REcult...

  6. Technical training - places available

    CERN Multimedia

    2012-01-01

    If you would like more information on a course, or for any other inquiry/suggestions, please contact Technical.Training@cern.ch Valeria Perez Reale, Learning Specialist, Technical Programme Coordinator (Tel.: 62424) Eva Stern and Elise Romero, Technical Training Administration (Tel.: 74924) HR Department »Electronics design Next Session Duration Language Availability Comprehensive VHDL for FPGA Design 08-Oct-12 to 12-Oct-12 5 days English 3 places available Foundations of Electromagnetism and Magnet Design (EMAG) 14-Nov-12 to 27-Nov-12 6 days English 20 places available Impacts de la suppression du plomb (RoHS) en électronique 26-Oct-12 to 26-Oct-12 8 hours French 15 places available Introduction to VHDL 10-Oct-12 to 11-Oct-12 2 days English 7 places available LabVIEW Real Time and FPGA 13-Nov-12 to 16-Nov-12 5 days French 5 places available »Mechanical design Next Se...

  7. Vectorial signalling mechanism required for cell-cell communication during sporulation in Bacillus subtilis.

    Science.gov (United States)

    Diez, Veronica; Schujman, Gustavo E; Gueiros-Filho, Frederico J; de Mendoza, Diego

    2012-01-01

    Spore formation in Bacillus subtilis takes place in a sporangium consisting of two chambers, the forespore and the mother cell, which are linked by pathways of cell-cell communication. One pathway, which couples the proteolytic activation of the mother cell transcription factor σ(E) to the action of a forespore synthesized signal molecule, SpoIIR, has remained enigmatic. Signalling by SpoIIR requires the protein to be exported to the intermembrane space between forespore and mother cell, where it will interact with and activate the integral membrane protease SpoIIGA. Here we show that SpoIIR signal activity as well as the cleavage of its N-terminal extension is strictly dependent on the prespore fatty acid biosynthetic machinery. We also report that a conserved threonine residue (T27) in SpoIIR is required for processing, suggesting that signalling of SpoIIR is dependent on fatty acid synthesis probably because of acylation of T27. In addition, SpoIIR localization in the forespore septal membrane depends on the presence of SpoIIGA. The orchestration of σ(E) activation in the intercellular space by an acylated signal protein provides a new paradigm to ensure local transmission of a weak signal across the bilayer to control cell-cell communication during development. © 2011 Blackwell Publishing Ltd.

  8. The comER Gene Plays an Important Role in Biofilm Formation and Sporulation in both Bacillus subtilis and Bacillus cereus.

    Science.gov (United States)

    Yan, Fang; Yu, Yiyang; Wang, Luyao; Luo, Yuming; Guo, Jian-Hua; Chai, Yunrong

    2016-01-01

    Bacteria adopt alternative cell fates during development. In Bacillus subtilis, the transition from planktonic growth to biofilm formation and sporulation is controlled by a complex regulatory circuit, in which the most important event is activation of Spo0A, a transcription factor and a master regulator for genes involved in both biofilm formation and sporulation. In B. cereus, the regulatory pathway controlling biofilm formation and cell differentiation is much less clear. In this study, we show that a novel gene, comER, plays a significant role in biofilm formation as well as sporulation in both B. subtilis and B. cereus. Mutations in the comER gene result in defects in biofilm formation and a delay in spore formation in the two Bacillus species. Our evidence supports the idea that comER may be part of the regulatory circuit that controls Spo0A activation. comER likely acts upstream of sda, a gene encoding a small checkpoint protein for both sporulation and biofilm formation, by blocking the phosphor-relay and thereby Spo0A activation. In summary, our studies outlined a conserved, positive role for comER, a gene whose function was previously uncharacterized, in the regulation of biofilm formation and sporulation in the two Bacillus species.

  9. The comER Gene Plays an Important Role in Biofilm Formation and Sporulation in both Bacillus subtilis and Bacillus cereus

    Science.gov (United States)

    Yan, Fang; Yu, Yiyang; Wang, Luyao; Luo, Yuming; Guo, Jian-hua; Chai, Yunrong

    2016-01-01

    Bacteria adopt alternative cell fates during development. In Bacillus subtilis, the transition from planktonic growth to biofilm formation and sporulation is controlled by a complex regulatory circuit, in which the most important event is activation of Spo0A, a transcription factor and a master regulator for genes involved in both biofilm formation and sporulation. In B. cereus, the regulatory pathway controlling biofilm formation and cell differentiation is much less clear. In this study, we show that a novel gene, comER, plays a significant role in biofilm formation as well as sporulation in both B. subtilis and B. cereus. Mutations in the comER gene result in defects in biofilm formation and a delay in spore formation in the two Bacillus species. Our evidence supports the idea that comER may be part of the regulatory circuit that controls Spo0A activation. comER likely acts upstream of sda, a gene encoding a small checkpoint protein for both sporulation and biofilm formation, by blocking the phosphor-relay and thereby Spo0A activation. In summary, our studies outlined a conserved, positive role for comER, a gene whose function was previously uncharacterized, in the regulation of biofilm formation and sporulation in the two Bacillus species. PMID:27446060

  10. Effect of different concentrations of dl-isoleucine, dl-valine, and dl-alanine on growth and sporulation in Fusarium oxysporum f. udum (Butl.) Sn. et H.

    Science.gov (United States)

    Prasad, M; Chaudhary, S K

    1977-01-01

    D1-alanine and dl-valine, when added as an extra nitrogen for fortifying the already present inorganic nitrogen source, actually acted as growth retardant for F. oxysporum f. udum (Butl.) Sn. et H. Sporulation of microconidia was indifferently affected by these two amino acids. DI-valine stimulated microconidial formation in young cultures only. In both young and old cultures the lowest concentration of dl-valine depressed macronidial sporulation. In old cultures the lowest concentration of valine stimulated chlamydospore differentiation rapidly, higher concentrations being less effective. D1-alanine, as an additional nitrogen source, depressed both macro- and microconidal sporulation. It did not even invigorate chlamydospore formation. D1-isoleucine, on the other hand, belongs to the category of growth promoters and profuse and stimulative sporulators of macro- and microconidia. This pathogen needs very specific and preferential doses of the three amino acids, if these are used as a booster in addition to the already present nitrogen source. The response, both in terms of mycelial growth and sporulation of the three spore forms, was also conditioned by the age of the culture.

  11. Where is 'place' in Aging in place?

    DEFF Research Database (Denmark)

    Blaakilde, Anne Leonora

    2015-01-01

    is influenced by universalism, aiming at equality in terms of access to health services and care. However, these welfare provisions seem to be deeply embedded in methodological nationalism, since only citizens with residence within the borders of Denmark have the right to live in public nursing homes or receive......The aim of this article is to contribute a transnational perspective to the field of environmental gerontology and the concept of aging in place. Seniors from the northern hemisphere, among them Danish citizens, are increasingly adapting to transnational lives as they move to warmer climates...... in-home help. It is argued that we should consider public solutions to the problems faced by frail Danish citizens in transnational settings, enhancing their opportunities to live abroad....

  12. Taking iron supplements

    Science.gov (United States)

    ... medlineplus.gov/ency/article/007478.htm Taking iron supplements To use the sharing features on this page, ... levels. You may also need to take iron supplements as well to rebuild iron stores in your ...

  13. Context in place

    DEFF Research Database (Denmark)

    Nordentoft, Helle Merete; Thomsen, Rie

    specifically argue for a more grounded approach to the conception of context - a topographic approach - in which the physical setting - i.e. 'the place' becomes an inevitable part of analyses of guidance practices in order to understand participants' sense-making processes. In the paper we draw on two case...... studies on interdisciplinary clinical supervision and work place guidance in which there appears to be a mismatch between intended outcomes and actual events. The analyses demonstrate and support that 'the place' seems - to influence partipants' responses in the guidance sessions and, therefore, must...

  14. Management and communication courses – Places available

    CERN Multimedia

    2013-01-01

    There are places available in some management and communication courses taking place in the period April to June 2013.   For more information on the course, click on the course title, this will bring you to the training catalogue. You can then sign-up online. For advice, you can contact: - Erwin Mosselmans, tel. 74125, erwin.mosselmans@cern.ch - Nathalie Dumeaux, tel. 78144, nathalie.dumeaux@cern.ch Courses in English (or bilingual) Sessions Duration Language Availability Managing stress 29 and 30 May 2 days English 3 places Making Presentations 30, 31 May & 25 June 3 days English 2 places Communicating Effectively - Residential course 4 to 6 June 3 days Bilingual 9 places Handling difficult conversations (Adapted from Dealing with Conflict) 7 and 14 June and 13 September 3 days English 6 places Voice and Nonverbal Behaviour in Speech Communication 17 and 18 June 1 day 4 hours English 7 plac...

  15. Technical training - Places available

    CERN Document Server

    2012-01-01

    If you would like more information on a course, or for any other inquiry/suggestions, please contact Technical.Training@cern.ch Valeria Perez Reale, Learning Specialist, Technical Programme Coordinator (Tel.: 62424) Eva Stern and Elise Romero, Technical Training Administration (Tel.: 74924)   Electronics design Next Session Duration Language Availability Certified LabVIEW Associate Developer (CLAD) 06-Dec-12 to 06-Dec-12 1 hour English One more place available Compatibilité électromagnetique (CEM): Applications 23-Nov-12 to 23-Nov-12 3.5 hours English 3 places available Compatibilité électromagnétique (CEM): Introduction 23-Nov-12 to 23-Nov-12 3 hours English 43 places available Effets des Radiations sur les composants et systèmes électroniques 11-Dec-12 to 12-Dec-12 1 day 4 hours French 9 places available LabVIEW for beginners ...

  16. History, Criticism and Place

    DEFF Research Database (Denmark)

    Hinds, Mat; Carter, Adrian; Malpas, Jeff

    2012-01-01

    Rory Spence and Richard Leplastrier shared a conversation and friendship that lasted 20years until Spence's death in 2004. The discussions focused largely upon issues of place, distilled through the practice of Leplastrier....

  17. Marine Place Names

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains the geographic place names for features in the U.S territorial waters and outer continental shelf. These names can be used to find or define a...

  18. Self-Placing Concrete

    OpenAIRE

    ECT Team, Purdue

    2007-01-01

    Certain concrete pours have areas where the congestion of reinforcing bars make placement of concrete almost impossible. Using conventional placing and vibration techniques, the resulting concrete can have considerable honeycombing due to the development of voids. Self-placing concrete is a possible solution to the problem. Also known as self-compactable concrete, self-consolidating concrete, flowable concrete, and non-vibration concrete. These concretes eliminate the need for vibration in a ...

  19. 'A Place to Sit'

    DEFF Research Database (Denmark)

    Hvejsel, Marie Frier; Klok, Julie Skovgaard; Bøhnke, Mia Marker

    Published in 2014 on the occasion of the third 'A Place to Sit' exhibition as a reflection upon three years of teaching tectonic method in architecture using the furniture scale as a learning basis.......Published in 2014 on the occasion of the third 'A Place to Sit' exhibition as a reflection upon three years of teaching tectonic method in architecture using the furniture scale as a learning basis....

  20. Technical training - Places available

    CERN Multimedia

    2012-01-01

    If you would like more information on a course, or for any other inquiry/suggestions, please contact Technical.Training@cern.ch Valeria Perez Reale, Learning Specialist, Technical Programme Coordinator (Tel.: 62424) Eva Stern and Elise Romero, Technical Training Administration (Tel.: 74924) HR Department Electronic Design Next Session Duration Language Availability Comprehensive VHDL for FPGA Design 08-Oct-12 to 12-Oct-12 5 days English 4 places Electrostatique / Protection ESD 28-Sep-12 to 28-Sep-12 3 hours French 25 places Impacts de la suppression du plomb (RoHS) en électronique 26-Oct-12 to 26-Oct-12 8 hours French 14 places Introduction to VHDL 10-Oct-12 to 11-Oct-12 2 days English 9 places LabVIEW Real Time and FPGA 13-Nov-12 to 16-Nov-12 5 days French 5 places LabVIEW for Experts 24-Sep-12 to 28-Sep-12 5 days English 6 places LabVIEW for beginners 15-Oct-12 to 17-...

  1. Kinetic modeling of sporulation and product formation in stationary phase by Bacillus coagulans RK-02 vis-à-vis other Bacilli.

    Science.gov (United States)

    Das, Subhasish; Sen, Ramkrishna

    2011-10-01

    A logistic kinetic model was derived and validated to characterize the dynamics of a sporogenous bacterium in stationary phase with respect to sporulation and product formation. The kinetic constants as determined using this model are particularly important for describing intrinsic properties of a sporogenous bacterial culture in stationary phase. Non-linear curve fitting of the experimental data into the mathematical model showed very good correlation with the predicted values for sporulation and lipase production by Bacillus coagulans RK-02 culture in minimal media. Model fitting of literature data of sporulation and product (protease and amylase) formation in the stationary phase by some other Bacilli and comparison of the results of model fitting with those of Bacillus coagulans helped validate the significance and robustness of the developed kinetic model. Copyright © 2011 Elsevier Ltd. All rights reserved.

  2. Immunizing potential of sporulated oocysts of Eimeria nieschulzi exposed to heat and 60Co gamma-radiation

    International Nuclear Information System (INIS)

    Conder, G.A.; Duszynski, D.W.

    1977-01-01

    Sporulated oocysts of Eimeria nieschulzi Dieben 1924, a rat coccidium, were exposed to radiation, heat, or both in an effort to attenuate the parasite. Moderate levels of each treatment or combination thereof attenuated the parasite, reduced pathogenesis (as judged by oocyst discharge during primary infection), and produced immunity to challenge when the oocysts were subsequently inoculated into rats. Thus, heat- and/or radiation-treated E. nieschulzi oocysts fed to rats could reduce pathogenesis during a primary infection and yet give good homologous protection

  3. The GCKIII Kinase Sps1 and the 14-3-3 Isoforms, Bmh1 and Bmh2, Cooperate to Ensure Proper Sporulation in Saccharomyces cerevisiae

    Science.gov (United States)

    Slubowski, Christian J.; Paulissen, Scott M.; Huang, Linda S.

    2014-01-01

    Sporulation in the budding yeast Saccharomyces cerevisiae is a developmental program initiated in response to nutritional deprivation. Sps1, a serine/threonine kinase, is required for sporulation, but relatively little is known about the molecular mechanisms through which it regulates this process. Here we show that SPS1 encodes a bona-fide member of the GCKIII subfamily of STE20 kinases, both through phylogenetic analysis of the kinase domain and examination of its C-terminal regulatory domain. Within the regulatory domain, we find Sps1 contains an invariant ExxxPG region conserved from plant to human GCKIIIs that we call the EPG motif; we show this EPG motif is important for SPS1 function. We also find that Sps1 is phosphorylated near its N-terminus on Threonine 12, and that this phosphorylation is required for the efficient production of spores. In Sps1, Threonine 12 lies within a 14-3-3 consensus binding sequence, and we show that the S. cerevisiae 14-3-3 proteins Bmh1 and Bmh2 bind Sps1 in a Threonine 12-dependent fashion. This interaction is significant, as BMH1 and BMH2 are required during sporulation and genetically interact with SPS1 in sporulating cells. Finally, we observe that Sps1, Bmh1 and Bmh2 are present in both the nucleus and cytoplasm during sporulation. We identify a nuclear localization sequence in Sps1 at amino acids 411–415, and show that this sequence is necessary and sufficient for nuclear localization. Taken together, these data identify regions within Sps1 critical for its function and indicate that SPS1 and 14-3-3s act together to promote proper sporulation in S. cerevisiae. PMID:25409301

  4. Studies on the control of development: isolation of Bacillus subtilis mutants blocked early in sporulation and defective in synthesis of highly phosphorylated nucleotides.

    Science.gov (United States)

    Rhaese, H J; Hoch, J A; Groscurth, R

    1977-03-01

    To test our model on the mechanism of initiation of differentiation in Bacillus subtilis, we tested early blocked (stage 0) sporulation mutants for their ability to synthesize highly phosphorylated nucleotides. We also isolated early blocked asporogenous mutants with the aid of the intercalating drug tilorone. Among all mutants tested we found that the spo0F-bearing strain was unable to synthesize adenosine 3'(2')-triphosphate 5'-triphosphate, pppAppp. A revertant of this mutant regained the ability to both sporulate and synthesize pppAppp. Ribosomes of the asporogenous mutant isolated at T2 (2 hr after the end of logarithmic growth) of sporulation, in contrast to the wild type, do not synthesize adenosine 3'(2')-diphosphate 5'-diphosphate, ppApp, or adenosine 3'(2')-diphosphate 5'-triphosphate, pppApp, but synthesize guanosine 3'(2')-diphosphate 5'-diphosphate, ppGpp, and guanosine 3'(2')-diphosphate 5'-triphosphate, pppGpp. This behavior is characteristic of ribosomes from vegetative, not sporulating, cells. Ribosomes from the sporogenous revertant behave like those of the wild type. The results suggest that the spo0F mutation may be a mutation in the structural gene for pppAppp synthetase. The inability to synthesize pppAppp in this strain also prevents the formation of "sporulation-specific ribosomes," i.e., ribosomes that synthetize ppApp and pppApp. The present experiments suggest that the nucleotide pppAppp participates in the initiation of sporulation by triggering a sequencies of events required for the production of heat-resistant spores.

  5. NMR studies of the sporulation protein SpoIIAA: Implications for the regulation of the transcription factor σF in Bacillus subtilis

    International Nuclear Information System (INIS)

    Kovacs, Helena; Comfort, David; Lord, Matthew; Yudkin, Michael; Campbell, Iain D.; Nilges, Michael

    2001-01-01

    SpoIIAA participates in a four-component mechanism for phosphorylation-dependent transcription control at the outset of sporulation. We report the refinement of the solution structure of SpoIIAA by using the automated iterative NOE assignment method ARIA. To complement the structural data, the protein dynamics were determined by measuring the T 1 , T 2 and NOE of the backbone 15 N-nuclei. The refined structure permits a discussion of the structural features that are important for the function of SpoIIAA in the regulation of the sporulation sigma factor σ F , and for homologous regulatory pathways present in B. subtilis and in other bacilli

  6. Alternative Sigma Factors SigF, SigE, and SigG Are Essential for Sporulation in Clostridium botulinum ATCC 3502

    OpenAIRE

    Kirk, David G.; Zhang, Zhen; Korkeala, Hannu; Lindström, Miia

    2014-01-01

    Clostridium botulinum produces heat-resistant endospores that may germinate and outgrow into neurotoxic cultures in foods. Sporulation is regulated by the transcription factor Spo0A and the alternative sigma factors SigF, SigE, SigG, and SigK in most spore formers studied to date. We constructed mutants of sigF, sigE, and sigG in C. botulinum ATCC 3502 and used quantitative reverse transcriptase PCR and electron microscopy to assess their expression of the sporulation pathway on transcription...

  7. (Re)tasting places

    DEFF Research Database (Denmark)

    Hedegaard, Liselotte

    2015-01-01

    What does geographical origin mean? It is an expression that associates food and wine with a specific place, an association embedded in the concept ‘terroir’ that refers to the complex interaction between a physical environment and local craftsmanship. It is a claim protected through labelling......-schemes and a claim that adds value to the place-related foods. However, viewing the connection between food and place as a question of proving a relationship or as a matter of protecting commercial claims does not seem to provide a satisfactory account for the status of geographically designated foods as being...... particularly attractive Central to the interest of this paper is to approach an understanding of geographical origin as a point of reference for taste. In terms of being sensory experience, taste is subjective. It is difficult to describe verbally and yet at the same time it is a trigger of the memory of past...

  8. Stress at Work Place

    OpenAIRE

    Mohammad A. Shahrour

    2010-01-01

    One of hardest forms of stresses to avoid is that work place or job stress Job stress refers to stress experienced by an individual at or because of issues at their work place The term work related stress has many meanings and it causes different levels of anxiety. Not all challenges at work can be called stress as some of these challenges drive employees upward, and empower them to learn new skills or push them to work harder to achieve a certain goal. So, this type of challenges cannot be c...

  9. Ageing in communal place

    DEFF Research Database (Denmark)

    Aarhus, Rikke; Ballegaard, Stinne Aaløkke; Grönvall, Erik

    2009-01-01

    In this paper we adopt the position that design of social media for the elderly and virtual senior communities may be informed by studying `real´senior communities. Since current research efforts target the role of social media and virtual communities for supporting seniors ageing in place, i.......e. in their homes, housing communities seems a natural place to begin this enquiry. We conducted observations and informal interviews in six different senior dwellings. In this paper we present the key findings from these visits related to social interaction and the formation of communities and explicate how...

  10. Structure of the sporulation histidine kinase inhibitor Sda from Bacillus subtilis and insights into its solution state

    Energy Technology Data Exchange (ETDEWEB)

    Jacques, David A.; Streamer, Margaret [School of Molecular and Microbial Biosciences, University of Sydney (Australia); Rowland, Susan L.; King, Glenn F. [Institute of Molecular Biology, University of Queensland (Australia); Guss, J. Mitchell; Trewhella, Jill; Langley, David B., E-mail: d.langley@usyd.edu.au [School of Molecular and Microbial Biosciences, University of Sydney (Australia)

    2009-06-01

    The crystal structure of Sda, a DNA-replication/damage checkpoint inhibitor of sporulation in B. subtilis, has been solved via the MAD method. The subunit arrangement in the crystal has enabled a reappraisal of previous biophysical data, resulting in a new model for the behaviour of the protein in solution. The crystal structure of the DNA-damage checkpoint inhibitor of sporulation, Sda, from Bacillus subtilis, has been solved by the MAD technique using selenomethionine-substituted protein. The structure closely resembles that previously solved by NMR, as well as the structure of a homologue from Geobacillus stearothermophilus solved in complex with the histidine kinase KinB. The structure contains three molecules in the asymmetric unit. The unusual trimeric arrangement, which lacks simple internal symmetry, appears to be preserved in solution based on an essentially ideal fit to previously acquired scattering data for Sda in solution. This interpretation contradicts previous findings that Sda was monomeric or dimeric in solution. This study demonstrates the difficulties that can be associated with the characterization of small proteins and the value of combining multiple biophysical techniques. It also emphasizes the importance of understanding the physical principles behind these techniques and therefore their limitations.

  11. Chemical sporulation and germination: cytoprotective nanocoating of individual mammalian cells with a degradable tannic acid-FeIII complex

    Science.gov (United States)

    Lee, Juno; Cho, Hyeoncheol; Choi, Jinsu; Kim, Doyeon; Hong, Daewha; Park, Ji Hun; Yang, Sung Ho; Choi, Insung S.

    2015-11-01

    Individual mammalian cells were coated with cytoprotective and degradable films by cytocompatible processes maintaining the cell viability. Three types of mammalian cells (HeLa, NIH 3T3, and Jurkat cells) were coated with a metal-organic complex of tannic acid (TA) and ferric ion, and the TA-FeIII nanocoat effectively protected the coated mammalian cells against UV-C irradiation and a toxic compound. More importantly, the cell proliferation was controlled by programmed formation and degradation of the TA-FeIII nanocoat, mimicking the sporulation and germination processes found in nature.Individual mammalian cells were coated with cytoprotective and degradable films by cytocompatible processes maintaining the cell viability. Three types of mammalian cells (HeLa, NIH 3T3, and Jurkat cells) were coated with a metal-organic complex of tannic acid (TA) and ferric ion, and the TA-FeIII nanocoat effectively protected the coated mammalian cells against UV-C irradiation and a toxic compound. More importantly, the cell proliferation was controlled by programmed formation and degradation of the TA-FeIII nanocoat, mimicking the sporulation and germination processes found in nature. Electronic supplementary information (ESI) available: Experimental details, LSCM images, and SEM and TEM images. See DOI: 10.1039/c5nr05573c

  12. THE EFFECT OF GROWTH PARAMETERS ON THE ANTIBIOTIC ACTIVITY AND SPORULATION IN BACILLUS SPP. ISOLATED FROM SOIL

    Directory of Open Access Journals (Sweden)

    Alev Usta

    2013-04-01

    Full Text Available Fifty-two Bacillus strains, which were isolated from different soil samples, were screened for antibiotic properties. The Bacillus strains were checked for antibacterial properties by the cross-streak method against 5 test pathogens, and 25 Bacillus strains had an effect on the test microorganisms. One strain of Bacillus, which exhibited the largest inhibition zone (25 mm against Shigella sonnei, was named Bacillus sp. EA62. The antibacterial activity from Bacillus sp. EA62 was tested in six different culture media against Shigella sonnei using the agar well diffusion method. The best activity medium was selected and used for further studies. The influence of the incubation period, pH, and different glucose and nitrogen concentrations on the antibacterial activity was studied. The optimal conditions for the strongest antibiotic activity were found to be 72 hours (18 mm, pH 7.5 (23 mm, 3% glucose (25 mm, and 0.3% nitrogen concentration (23 mm. Additionally, the relationship between the antibiotic activity and sporulation was investigated. Accordingly, it was determined that the increase of the activity paralleled sporulation.

  13. ParA and ParB coordinate chromosome segregation with cell elongation and division during Streptomyces sporulation

    Science.gov (United States)

    Donczew, Magdalena; Mackiewicz, Paweł; Wróbel, Agnieszka; Flärdh, Klas; Zakrzewska-Czerwińska, Jolanta

    2016-01-01

    In unicellular bacteria, the ParA and ParB proteins segregate chromosomes and coordinate this process with cell division and chromosome replication. During sporulation of mycelial Streptomyces, ParA and ParB uniformly distribute multiple chromosomes along the filamentous sporogenic hyphal compartment, which then differentiates into a chain of unigenomic spores. However, chromosome segregation must be coordinated with cell elongation and multiple divisions. Here, we addressed the question of whether ParA and ParB are involved in the synchronization of cell-cycle processes during sporulation in Streptomyces. To answer this question, we used time-lapse microscopy, which allows the monitoring of growth and division of single sporogenic hyphae. We showed that sporogenic hyphae stop extending at the time of ParA accumulation and Z-ring formation. We demonstrated that both ParA and ParB affect the rate of hyphal extension. Additionally, we showed that ParA promotes the formation of massive nucleoprotein complexes by ParB. We also showed that FtsZ ring assembly is affected by the ParB protein and/or unsegregated DNA. Our results indicate the existence of a checkpoint between the extension and septation of sporogenic hyphae that involves the ParA and ParB proteins. PMID:27248800

  14. Saprophytic Activity and Sporulation of Cryphonectria parasitica on Dead Chestnut Wood in Forests with Naturally Established Hypovirulence.

    Science.gov (United States)

    Prospero, S; Conedera, M; Heiniger, U; Rigling, D

    2006-12-01

    ABSTRACT Sustainable biological control of the chestnut blight fungus Crypho-nectria parasitica with hypovirulence depends on the production and dissemination of hypovirus-infected propagules of the pathogen. We investigated the ability of C. parasitica to sporulate and produce hypo-virus-infected spores on recently dead chestnut wood in coppice stands in southern Switzerland where hypovirulence has been naturally established. The number and type (active, inactive, or none) of cankers was assessed on experimentally cut and stacked stems, firewood stacks, and natural dead wood. Hypovirus-free and hypovirus-infected strains readily survived for more than 1 year in the chestnut blight cankers of the stacked stems. Sporulation of C. parasitica was observed on the surface of preexisting inactive and active cankers, as well as on newly colonized bark areas and was significantly more abundant than on comparable cankers on living stems. On all types of dead wood, we observed more stromata with perithecia than with pycnidia; however, a large proportion of the stromata was not differentiated. All perithecia examined yielded only hypovirus-free ascospores. The incidence of pycnidia that produced hypovirus-infected conidia ranged from 5% on natural dead wood to 41% on the experimental stacks. The mean virus transmission rate into conidia was 69%. Our study demonstrates a considerable saprophytic activity of C. parasitica on recently dead chestnut wood and supports the hypothesis of a role of this saprophytic phase in the epidemiology of hypovirulence.

  15. Ras GTPases Modulate Morphogenesis, Sporulation and Cellulase Gene Expression in the Cellulolytic Fungus Trichoderma reesei

    Science.gov (United States)

    Zhang, Jiwei; Zhang, Yanmei; Zhong, Yaohua; Qu, Yinbo; Wang, Tianhong

    2012-01-01

    Background The model cellulolytic fungus Trichoderma reesei (teleomorph Hypocrea jecorina) is capable of responding to environmental cues to compete for nutrients in its natural saprophytic habitat despite its genome encodes fewer degradative enzymes. Efficient signalling pathways in perception and interpretation of environmental signals are indispensable in this process. Ras GTPases represent a kind of critical signal proteins involved in signal transduction and regulation of gene expression. In T. reesei the genome contains two Ras subfamily small GTPases TrRas1 and TrRas2 homologous to Ras1 and Ras2 from S. cerevisiae, but their functions remain unknown. Methodology/Principal Findings Here, we have investigated the roles of GTPases TrRas1 and TrRas2 during fungal morphogenesis and cellulase gene expression. We show that both TrRas1 and TrRas2 play important roles in some cellular processes such as polarized apical growth, hyphal branch formation, sporulation and cAMP level adjustment, while TrRas1 is more dominant in these processes. Strikingly, we find that TrRas2 is involved in modulation of cellulase gene expression. Deletion of TrRas2 results in considerably decreased transcription of cellulolytic genes upon growth on cellulose. Although the strain carrying a constitutively activated TrRas2G16V allele exhibits increased cellulase gene transcription, the cbh1 and cbh2 expression in this mutant still strictly depends on cellulose, indicating TrRas2 does not directly mediate the transmission of the cellulose signal. In addition, our data suggest that the effect of TrRas2 on cellulase gene is exerted through regulation of transcript abundance of cellulase transcription factors such as Xyr1, but the influence is independent of cAMP signalling pathway. Conclusions/Significance Together, these findings elucidate the functions for Ras signalling of T. reesei in cellular morphogenesis, especially in cellulase gene expression, which contribute to deciphering the

  16. Technical training - Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 16.5.2006 (May-November course sessions) Technical Training: Places available The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Title Hours Date Language ACROBAT 7.0 : Utilisation de fichiers PDF 8 8.05.06 F WORD 2003 - niveau 2 : ECDL 16 22-23.05.06 23-24.05.06 F Comprehensive VHDL for FPGA Design 40 29.05-2.06.06 E C++ Programming Part 2 - Advanced C++ and its Traps and Pitfalls 32 30.05-2.06.06 E ACROBAT 7.0 : Utilisation de fichiers PDF 24 7-9.06.06 E AutoCAD Mechanical 2006 16 13-14.06.06 F CERN EDMS for Local Administrators 16 13-14.06.06 E LabVIEW Base 2 32 27.06-5.07.06 F C++ Programming Part 3 - Templates and the STL (Standard Template Library) 16 27-28.06.06 E C++ Programming Part 4 - Exceptions 8 29.06.06 E FrontPage 2003 - niveau 1 16 29-...

  17. The Value of Place

    Science.gov (United States)

    Dentzau, Michael W.

    2014-01-01

    This commentary seeks to expand the dialogue on place-based science education presented in Katie Lynn Brkich's article, where the connections fifth grade students make between their formal earth science curriculum and their lived experiences are highlighted. The disconnect between the curriculum the students are offered and their immediate…

  18. The power of place.

    Science.gov (United States)

    Lock, Lin R; Gibb, Heather J

    2003-06-01

    to describe the power that 'place' holds over the postnatal-care experiences of women. a study informed by phenomenology within a feminist framework was undertaken to examine the experiences of women electing early postnatal discharge. Three extended conversations with each woman participating in the study were audiotaped and transcribed. Journal notes made by the researcher added to the audiotaped data. Thematic analysis revealed major structures of experience. data were obtained from conversations with women in their respective homes. five women, parity 1-3, living in the Sydney metropolitan area and birthing in their local hospitals participated in the study. four major constructs of experience were revealed through analysis and include spatiality, corporeality, temporality and relationality. In this paper, components of spatiality expressed through the power place exerts in matters of physical environment,control, confidence, safety, time, talk and the heart of the matter are presented. the experiences of women entering the foreign place of hospital to birth their children were those of alienation and disempowerment while the familiar territory of home offered stronger feelings of security and support. failing to recognise the impact of place on the experiences of postnatal women reduces the likelihood that midwives will be able to offer sensitive and appropriate care.

  19. Ageing in Communal Place

    DEFF Research Database (Denmark)

    Aarhus, Rikke; Ballegaard, Stinne Aaløkke; Grönvall, Erik

    2009-01-01

    In this paper we adopt the position that design of social media for the elderly and virtual senior communities may be informed by studying ‘real’ senior communities. Since current research efforts target the role of social media and virtual communities for supporting seniors ageing in place, i...

  20. Technical training - Places available

    CERN Multimedia

    2003-01-01

    * Etant donné le délai d'impression du Bulletin, ces places peuvent ne plus être disponibles au moment de sa parution. Veuillez consulter notre site Web pour avoir la dernière mise à jour. ** The number of places available may vary. Please check our Web site to find out the current availability. Des places sont disponibles dans les cours suivants : Places are available in the following courses: Hands-on Introduction to Python Programming 12 – 14.11.03 (3 days) ACCESS 2000 – niveau 1 13 & 14.11.03 (2 jours) C++ for Particle Physicists 17 – 21.11.03 (6 x 3-hour lectures) Programmation automate Schneider TSX Premium – niveau 2  18 – 21.11.03 (4 jours) Planification de projet avec MS-Project/Project Planning with MS-Project (gratuit/free of charge – langue à définir/language to be defined) : 18 & 25.11.03 (2 jours/2 days) JAVA 2 Enterprise Edition – Part 1 : WEB...

  1. How to take statins

    Science.gov (United States)

    ... allergies. You are taking other medicines. You have diabetes. You have liver disease. You should not take statins if you ... with your provider about the possible risks for: Liver damage Severe ... High blood sugar, or type 2 diabetes Memory loss Confusion

  2. Note Taking and Recall

    Science.gov (United States)

    Fisher, Judith L.; Harris, Mary B.

    1974-01-01

    To study the effect of note taking and opportunity for review on subsequent recall, 88 college students were randomly assigned to five treatment groups utilizing different note taking and review combinations. No treatment effects were found, although quality of notes was positively correlated with free recall an multiple-choice measures.…

  3. Settings: In a Variety of Place. . .

    Science.gov (United States)

    Cairo, Peter; And Others

    This document consists of the fourth section of a book of readings on issues related to adult career development. The four chapters in this fourth section focus on settings in which adult career development counseling may take place. "Career Planning and Development in Organizations" (Peter Cairo) discusses several concepts and definitions…

  4. Experiencing the enchantment of place and mobility

    DEFF Research Database (Denmark)

    Bærenholdt, Jørgen Ole

    2016-01-01

    in several layers of reality. To better understand experiences taking place in intersections between realities, J.R.R. Tolkien’s concept of how real enchantment produces a Secondary World suggests that we see fantasy as real, and this proposition is compared to Georg Simmel’s more modernist suggestion......Experiences of place and mobility play central roles not only in what was traditionally understood as tourism, but also in the broader practices of travelling and visiting sites and sights. On the one hand, such experiences are performed to an extent where it is difficult to isolate the sites...... and movements experienced per se, since visitors and travellers take part in ‘doing’ places and mobility. On the other, experience sites and routes stand out with specific traces and characteristics affording some – and not other – experiences. This paper discusses conceptual understandings that may help...

  5. Parks, People and Places

    DEFF Research Database (Denmark)

    Molin, Julie Frøik

    This thesis suggests, by applying a ‘place’ perspective, that involving users in operational management of green spaces holds potentials for enhancing ‘place attachment’ of urban inhabitants. As this can also build their commitment to decision making on their local living environment, green space...... maintenance becomes a potentially important setting for governance processes. Through a number of qualitative case studies, based on semi-structured interviews with municipal and community actors in Denmark and England, this dissertation explores the status and potentials of applying a place-based governance...... approach to green space maintenance. To guide the exploration a theoretical framework was developed inductively, structured around concepts of environmental governance in combination with strategic approaches to green space management. The framework is operationalised by analyses of the governance...

  6. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The Joint PVSS JCOP Framework: 14 - 18.6.2004 (5 days) EXCEL 2003 - niveau 2 : 17 & 18.6.2004 (2 jours) MAGNE-04 : Magnétisme pour l'électrotechnique : 6 au 8.7.2004 (3 jours) Technical Training Monique Duval - Tel.74924 technical.training@cern.ch

  7. Sporulation-specific cell division defects in ylmE mutants of Streptomyces coelicolor are rescued by additional deletion of ylmD.

    Science.gov (United States)

    Zhang, Le; Willemse, Joost; Hoskisson, Paul A; van Wezel, Gilles P

    2018-05-09

    Cell division during the reproductive phase of the Streptomyces life-cycle requires tight coordination between synchronous formation of multiple septa and DNA segregation. One remarkable difference with most other bacterial systems is that cell division in Streptomyces is positively controlled by the recruitment of FtsZ by SsgB. Here we show that deletion of ylmD (SCO2081) or ylmE (SCO2080), which lie in operon with ftsZ in the dcw cluster of actinomycetes, has major consequences for sporulation-specific cell division in Streptomyces coelicolor. Electron and fluorescence microscopy demonstrated that ylmE mutants have a highly aberrant phenotype with defective septum synthesis, and produce very few spores with low viability and high heat sensitivity. FtsZ-ring formation was also highly disturbed in ylmE mutants. Deletion of ylmD had a far less severe effect on sporulation. Interestingly, the additional deletion of ylmD restored sporulation to the ylmE null mutant. YlmD and YlmE are not part of the divisome, but instead localize diffusely in aerial hyphae, with differential intensity throughout the sporogenic part of the hyphae. Taken together, our work reveals a function for YlmD and YlmE in the control of sporulation-specific cell division in S. coelicolor, whereby the presence of YlmD alone results in major developmental defects.

  8. Sensor kinase KinB and its pathway-associated key factors sense the signal of nutrition starvation in sporulation of Bacillus subtilis.

    Science.gov (United States)

    Liu, Weipeng; He, Zeying; Gao, Feng; Yan, Jinyuan; Huang, Xiaowei

    2018-01-03

    Bacillus subtilis responds to environmental stress cues and develops endospores for survival. In the process of endospore formation, sporulation initiation is a vital stage and this stage is governed by autophosphorylation of the sensor histidine kinases. The second major sensor kinase KinB perceives the intracellular changes of GTP and ATP during sporulation. However, determination of the environmental signals as well as its related signaling pathway of KinB requires further elucidation. Our current study found that, contrary to the sporulation failure induced by ΔkinA in the nutrient-rich 2× SG medium, the sensor kinase KinB sensed the environmental cues in the nutrient-poor MM medium. Two other membrane proteins, KapB and KbaA, also responded similarly to the same external signal as KinB. Both KapB and KbaA acted upstream of KinB, but they exerted their regulation upon KinB independently. Furthermore, we demonstrated that both the SH3 domain and the α-helix structure in KapB are required for sensing or transducing the signal of sporulation initiation. Collectively, our work here supplied the direct evidences that KinB and its pathway sense the external signal of nutrient starvation in MM medium, and further analyzes the interrelationship among KinB, KbaA, and KapB. © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

  9. Dynamic sporulation gene co-expression networks for Bacillus subtilis 168 and the food-borne isolate Bacillus amyloliquefaciens : a transcriptomic model

    NARCIS (Netherlands)

    Omony, Jimmy; de Jong, Anne; Krawczyk, Antonina O; Eijlander, Robyn T; Kuipers, Oscar P

    2018-01-01

    Sporulation is a survival strategy, adapted by bacterial cells in response to harsh environmental adversities. The adaptation potential differs between strains and the variations may arise from differences in gene regulation. Gene networks are a valuable way of studying such regulation processes and

  10. Single cell analysis of gene expression patterns of competence development and initiation of sporulation in Bacillus subtilis grown on chemically defined media

    NARCIS (Netherlands)

    Veening, J. -W.; Smits, W. K.; Hamoen, L. W.; Kuipers, O. P.

    Aim: Understanding the basis for the heterogeneous (or bistable) expression patterns of competence development and sporulation in Bacillus subtilis. Methods and Results: Using flow cytometric analyses of various promoter-GFP fusions, we have determined the single-cell gene expression patterns of

  11. Comparison of expression of key sporulation, solventogenic and acetogenic genes in C. beijerinckii NRRL B-598 and its mutant strain overexpressing spo0A

    NARCIS (Netherlands)

    Kolek, J.; Diallo, M.; Vasylkivska, M.; Branska, B.; Sedlar, K.; López-Contreras, A.M.; Patakova, P.

    2017-01-01

    The production of acetone, butanol and ethanol by fermentation of renewable biomass has potential to become a valuable industrial process. Mechanisms of solvent production and sporulation involve some common regulators in some ABE-producing clostridia, although details of the links between the

  12. Clonostachys rosea reduces spot blotch in barley by inhibiting pre-penetration growth and sporulation of Bipolaris sorokiniana without inducing resistance

    DEFF Research Database (Denmark)

    Jensen, Birgit; Lübeck, Peter S; Jørgensen, Hans Jørgen Lyngs

    2016-01-01

    as the pathogen, whereas application 24 h after the pathogen had no effect. IK726 also reduced the sporulation capacity of B. sorokiniana. Microscopy of B. sorokiniana infection revealed that IK726 primarily inhibited conidial germination and appressorium formation while further pathogen development and host...

  13. A Gene Expressed during Sexual and Asexual Sporulation in Phytophthora infestans is a Member of the Puf Family of Translational Regulators

    DEFF Research Database (Denmark)

    Cvitanich, Cristina; Judelson, Howard S.

    2003-01-01

    and persisted in mature oospores. Expression was also observed in hyphal tips just prior to asexual sporulation, in sporangiophores, in mature sporangia, and in zoospores. The signal quickly disappeared once spores made the transition to hyphae after germination. Nutrient limitation did not induce the gene...

  14. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 30.5.2006 (June-November course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Titre Heure Date Langue ACROBAT 7.0 : Utilisation de fichiers PDF 8 6.06.06 F Introduction à InDesign 16 7-8.06.06 F Python: Hands-on Introduction 24 7-9.06.06 E LabVIEW Base 2 16 22-23.06.06 F FileMaker - niveau 1 16 26-27.06.06 F C++ Programming Part 3 - Templates and the STL (Standard Template Library) 16 27-28.06.06 E C++ Programming Part 4 - Exceptions 8 29.06.06 E FrontPage 2003 - niveau 1 16 29-30.06.06 F Manipulation des images 4 6.07.06 F Introduction to Databases and Database Design 16 11-12.07.06 E ACCESS 2003 - Level 1: ECDL 16 13-14.07.06 E-F Design Patterns 16 25-26.07.06 E Introduction à Dreamweaver MX 16 ...

  15. Technical training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 13.6.2006 (June-December course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Titre Heure Date Langue LabVIEW Base 2 16 22-23.06.06 F FileMaker - niveau 1 16 26-27.06.06 F C++ Programming Part 3 - Templates and the STL (Standard Template Library) 16 27-28.06.06 E C++ Programming Part 4 - Exceptions 8 29.06.06 E FrontPage 2003 - niveau 1 16 29-30.06.06 F Manipulation des images 4 6.07.06 F Introduction to Databases and Database Design 16 11-12.07.06 E ACCESS 2003 - Level 1: ECDL 16 13-14.07.06 E-F Design Patterns 16 25-26.07.06 E Introduction à Dreamweaver MX 16 26-27.07.06 F ANSYS DesignModeler 16 29-30.08.06 F LabVIEW Basics 1 24 4-6.09.06 E ANSYS Workbench 32 12-15.09.06 F AutoCAD Mechanical 20...

  16. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 9.5.2006 (May-October course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: find out the curren Hours Date Language LabVIEW Application Development 24 15-17.05.06 E LabVIEW Advanced Programming 16 18-19.05.06 E PERL 5: Advanced Aspects 8 18.05.06 E Technique du vide 16 18-19.05.06 F FileMaker - niveau 2 16 11-12.05.06 F WORD 2003 - niveau 2 : ECDL 16 22-23.05.06 F Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE 16 23-24.05.06 F Comprehensive VHDL for FPGA Design 40 29.05-2.06.06 E C++ Programming Part 2 - Advanced C++ and its Traps and Pitfalls 32 30.05-2.06.06 E Python: Hands-on Introduction 24 7-9.06.06 E AutoCAD Mechanical 2006 16 13-14.06.06 F CERN EDMS for Local Administrators 16 13-14.06.06...

  17. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 27.6.2006 (July-December course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Titre Heure Date Langue Manipulation des images 4 6.07.06 F Introduction to Databases and Database Design 16 11-12.07.06 E ACCESS 2003 - Level 1: ECDL 16 13-14.07.06 E-F Design Patterns 16 25-26.07.06 E CERN EDMS for Local Administrators 16 1-2.08.06 E ANSYS DesignModeler 16 29-30.08.06 F CERN EDMS - Introduction 8 5.09.06 E CERN EDMS MTF en pratique 4 6.09.06 F LabVIEW Basics 1 24 4-6.09.06 E ANSYS Workbench 32 12-15.09.06 F AutoCAD Mechanical 2006 16 12-13.09.06 F CERN EDMS for Engineers 8 12.09.06 E Software Engineering in the Small and the Large 16 12-13.09.06 E AutoCAD 2006 - niveau 1 32 14-21.09.06 F LabVIEW Basics 2 ...

  18. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 11.7.2006 (July-December course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Titre Heure Date Langue Design Patterns 16 25-26.07.06 E CERN EDMS for Local Administrators 16 1-2.08.06 E ANSYS DesignModeler 16 29-30.08.06 F CERN EDMS – Introduction 8 5.09.06 E CERN EDMS MTF en pratique 4 6.09.06 F LabVIEW Basics 1 24 4-6.09.06 E ANSYS Workbench 32 12-15.09.06 F AutoCAD Mechanical 2006 16 12-13.09.06 F CERN EDMS for Engineers 8 12.09.06 E Software Engineering in the Small and the Large 16 12-13.09.06 E LabVIEW Basics 2 16 14-15.09.06 E LabVIEW: Working efficiently with LabWIEW 8 8 18.09.06 E PCAD Schémas ? Introduction 16 21-22.09.06 F PCAD PCB - Introduction  24 27-29.09.06 F C++ for Particle Physicists ...

  19. Technical Training: Places available

    CERN Multimedia

    2006-01-01

    Places available as of 21.3.2006 (March-October course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: find out the curren Hours Date Language ACROBAT 7.0 : Utilisation de fichiers PDF 8 8.05.06 F Project Planning with MS-Project 16 9.05-6.06.06 E STEP7: niveau 1 32 9-12.05.06 E-F Oracle: Programming with PL/SQL 24 10-12.05.06 E FileMaker - niveau 2 16 11-12.05.06 F LabVIEW Application Development 24 15-17.05.06 E LabVIEW Advanced Programming 16 18-19.05.06 E PERL 5: Advanced Aspects 8 18.05.06 E Technique du vide 16 18-19.05.06 F WORD 2003 - niveau 2 : ECDL 16 22-23.05.06 F Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE 16 23-24.05.06 F Comprehensive VHDL for FPGA Design 40 29.05-2.06.06 E C++ Programming Part 2 - Advanced C++ and its T...

  20. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 19.7.2006 (August-December course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Titre Heure Date Langue CERN EDMS for Local Administrators 16 1-2.08.06 E ANSYS DesignModeler 16 29-30.08.06 F OUTLOOK 2003 (Short Course I) - E-mail 3 1.09.06 E/F OUTLOOK 2003 (Short Course II) - Calendar, Tasks and Notes 3 1.09.06 E/F CERN EDMS - Introduction 8 5.09.06 E CERN EDMS MTF en pratique 4 6.09.06 F LabVIEW Basics 1 24 11-13.09.06 E ANSYS Workbench 32 12-15.09.06 F CERN EDMS for Engineers 8 12.09.06 E Software Engineering in the Small and the Large 16 12-13.09.06 E LabVIEW Basics 2 16 14-15.09.06 E EXCEL 2003 (Short Course I) - HowTo... Work with formulae 3 15.09.06 E/F WORD 2003 (Short Course III) - HowTo... Work with long docu...

  1. Technical Training: Places available

    CERN Multimedia

    DAvide Vitè

    2006-01-01

    Places available as of 25.7.2006 (August-December course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Titre Heure Date Langue CERN EDMS for Local Administrators 16 1-2.08.06 E ANSYS DesignModeler 16 29-30.08.06 F EXCEL 2003 - niveau 1 : ECDL 16 30-31.08.06 F OUTLOOK 2003 (Short Course I) - E-mail 3 1.09.06 E/F OUTLOOK 2003 (Short Course II) - Calendar, Tasks and Notes 3 1.09.06 E/F CERN EDMS - Introduction 8 5.09.06 E CERN EDMS MTF en pratique 4 6.09.06 F ANSYS Workbench 32 12-15.09.06 F CERN EDMS for Engineers 8 12.09.06 E Software Engineering in the Small and the Large 16 12-13.09.06 E LabVIEW Basics 2 16 14-15.09.06 E WORD 2003 (Short Course III) - HowTo... Work with long documents 3 15.09.06 E/F EXCEL 2003 (Short Course I) - HowTo... Wor...

  2. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 7.2.2006 (February-May course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Title Hours Date Language WORD 2003 (Short Course II) - HowTo... Mail merge 3 09-02-06 E-F ACCESS 2003 - Level 1: ECDL M5 16 13 to 14-02-06 E-F OUTLOOK 2003 (Short Course II) - Calendar, Tasks and Notes 3 16-02-06 E-F WORD 2003 (Short Course III) - HowTo... Work with long documents 3 16-02-06 E-F CERN EDMS - Introduction 8 21.02.06 E OUTLOOK 2003 (Short Course III) - Meetings and Delegation 3 27-02-06 E-F WORD 2003 (Short Course IV) - HowTo... Work with master document 3 27-02-06 E-F JAVA: Level 2 32 28-02-06 to 03-03-06 E Manipulation des images 4 28.02.06 F ACCESS 2003 - Level 2: ECDL AM5 16 02 to 03-03-06 E-F FrontPage 2003 - niveau 2 16 02 to 03-03-06 F C++ for Particle Physicists 20 06 to 10-03-06 E FileMaker - niv...

  3. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 21.3.2006 (March-October course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Title Hours Date Language FrontPage 2003 - niveau 1 16 27-28.03.06 F Oracle Forms Developer 10g: Move to the Web 16 27-28.03.06 E ACCESS 2003 - Level 2: ECDL AM5 16 3-4.03.06 E-F JAVA 2 Enterprise Edition - Part 1: Web Applications 16 3-4.04.06 E JAVA 2 Enterprise Edition - Part 2: Enterprise JavaBeans 24 5-7.04.06 E AutoCAD Mechanical 2006 16 11-12.04.06 F FrontPage 2003 - niveau 2 16 24-25.04.06 F C++ Programming Part 1 - Introduction to Object-Oriented Design and Programming 24 25-27.04.06 E AutoCAD 2006 - niveau 1 32 27.04-4.05.06 F Oracle: SQL 24 3-5.05.06 E EXCEL 2003 (Short Course I) - HowTo... Work with formulae 3 4.05.06 (am) E-F EXCEL 2003 (Short Course II) - HowTo... Format your worksheet for printing 3 4...

  4. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 7.2.2006 (February-May course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Title Hours Date Language WORD 2003 (Short Course II) - HowTo... Mail merge 3 13.03.06 E-F EXCEL 2003 (Short Course III) - HowTo... Pivot tables 3 20.03.06 E-F EXCEL 2003 (Short Course IV) - HowTo....Link cells, worksheets and workbooks 3 20.03.06 E-F Object-Oriented Analysis and Design using UML 24 21-23.03.06 E EXCEL 2003 - niveau 1 16 22-23.03.06 F FrontPage 2003 - niveau 1 16 27-28.03.06 F Oracle Forms Developer 10g: Move to the Web 16 27-28.03.06 E Oracle JDeveloper 10g: Build Applications with ADF 24 29-31.03.06 E ACCESS 2003 - Level 2: ECDL AM5 16 3-4.03.06 E-F JAVA 2 Enterprise Edition - Part 1: Web Applications 16 3-4.04.06 E JCOP: Control System Integration using JCOP Tools 24 4-6.04.06 E JAVA 2 Enterprise Edition...

  5. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    Places available as of 7.2.2006 (February-May course sessions) The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Title Hours Date Language WORD 2003 (Short Course IV) - HowTo... Work with master document 3 27.02.06 E-F JAVA: Level 2 32 28.02-3.03.06 E Manipulation des images 4 28.02.06 F ACCESS 2003 - Level 2: ECDL AM5 16 2-3.03.06 E-F C++ for Particle Physicists 20 6-10.03.06 E PowerPoint 2003 8 9.03.06 F JCOP: Control System Integration using JCOP Tools 24 14-16.03.06 E EXCEL 2003 (Short Course III) - HowTo... Pivot tables 3 20.03.06 E-F EXCEL 2003 (Short Course IV) - HowTo....Link cells, worksheets and workbooks 3 20.03.06 E-F JCOP: Finite State Machines in the JCOP Framework 24 21-23.03.06 E Object-Oriented Analysis and Design using UML 24 21-23.03.06 E FrontPage 2003 - niveau 1 16 27-28.03.06 F JCOP: Joint PVSS-JCOP Fram...

  6. 1. On note taking.

    Science.gov (United States)

    Plaut, Alfred B J

    2005-02-01

    In this paper the author explores the theoretical and technical issues relating to taking notes of analytic sessions, using an introspective approach. The paper discusses the lack of a consistent approach to note taking amongst analysts and sets out to demonstrate that systematic note taking can be helpful to the analyst. The author describes his discovery that an initial phase where as much data was recorded as possible did not prove to be reliably helpful in clinical work and initially actively interfered with recall in subsequent sessions. The impact of the nature of the analytic session itself and the focus of the analyst's interest on recall is discussed. The author then describes how he modified his note taking technique to classify information from sessions into four categories which enabled the analyst to select which information to record in notes. The characteristics of memory and its constructive nature are discussed in relation to the problems that arise in making accurate notes of analytic sessions.

  7. A novel marine algal toxicity bioassay based on sporulation inhibition in the green macroalga Ulva pertusa (Chlorophyta)

    Energy Technology Data Exchange (ETDEWEB)

    Han, Taejun [Division of Biology and Chemistry, University of Incheon, Incheon 402-749 (Korea, Republic of)]. E-mail: hanalgae@incheon.ac.kr; Choi, Gye-Woon [Department of Civil and Environmental System Engineering, University of Incheon, Incheon 402-749 (Korea, Republic of)

    2005-11-10

    A 5-day aquatic toxicity test based on sporulation inhibition of the green macroalga Ulva pertusa Kjellman has been developed. Optimal test conditions determined for photon irradiance, salinity and temperature were 60-200 {mu}mol photons m{sup -2} s{sup -1}, 25-35%o and 15-20 deg C, respectively. Tests were conducted by exposing U. pertusa thallus disks to a reference toxicant (sodium dodecyl sulfate; SDS), metals (Cd{sup 2+}, Cu{sup 2+}, Zn{sup 2+}, Pb{sup 2+}) and elutriates of sludge collected from nine different locations. The EC{sub 50} values for SDS was 5.35 mg L{sup -1}. When four heavy metals were assayed, the NOECs were highest for lead (0.625 mg L{sup -1}) and lowest for copper (0.031 mg L{sup -1}). The EC{sub 50} values showed the following toxicity rankings: Cu{sup 2+} (0.061 mg L{sup -1}) > Cd{sup 2+} (0.326 mg L{sup -1}) > Zn{sup 2+} (0.738 mg L{sup -1}) > Pb{sup 2+} (0.877 mg L{sup -1}). The bioassay indicated also that the sporulation endpoint could be a sensitive indicator of toxicity effects of elutriates of sludge as reflected from the NOEC values equal to or lower than the lowest concentration employed (6.25%). Sporulation was significantly inhibitied in all elutriates with the greatest and least effects observed in elutriates of sludge from industrial waste (EC{sub 50} 6.78%) and filtration bed (EC{sub 50} 15.0%), respectively. The results of the Spearman rank correlation analysis for EC{sub 50} data versus the concentrations of toxicants in the sludge presented a significant correlation between toxicity and four heavy metals (Cd{sup 2+}, Cu{sup 2+}, Pb{sup 2+}, Zn{sup 2+}). Introduction of the concept of toxicity unit (TU) showed that these metals were the main cause of toxicity in elutriates of at least four out of nine sludge samples. Members of the order Ulvales show a wide geographic distribution and have similar reproductive characteristics, thus making it possible to apply the present test method to other algae of this taxa, elsewhere

  8. A novel marine algal toxicity bioassay based on sporulation inhibition in the green macroalga Ulva pertusa (Chlorophyta)

    International Nuclear Information System (INIS)

    Han, Taejun; Choi, Gye-Woon

    2005-01-01

    A 5-day aquatic toxicity test based on sporulation inhibition of the green macroalga Ulva pertusa Kjellman has been developed. Optimal test conditions determined for photon irradiance, salinity and temperature were 60-200 μmol photons m -2 s -1 , 25-35%o and 15-20 deg C, respectively. Tests were conducted by exposing U. pertusa thallus disks to a reference toxicant (sodium dodecyl sulfate; SDS), metals (Cd 2+ , Cu 2+ , Zn 2+ , Pb 2+ ) and elutriates of sludge collected from nine different locations. The EC 50 values for SDS was 5.35 mg L -1 . When four heavy metals were assayed, the NOECs were highest for lead (0.625 mg L -1 ) and lowest for copper (0.031 mg L -1 ). The EC 50 values showed the following toxicity rankings: Cu 2+ (0.061 mg L -1 ) > Cd 2+ (0.326 mg L -1 ) > Zn 2+ (0.738 mg L -1 ) > Pb 2+ (0.877 mg L -1 ). The bioassay indicated also that the sporulation endpoint could be a sensitive indicator of toxicity effects of elutriates of sludge as reflected from the NOEC values equal to or lower than the lowest concentration employed (6.25%). Sporulation was significantly inhibitied in all elutriates with the greatest and least effects observed in elutriates of sludge from industrial waste (EC 50 6.78%) and filtration bed (EC 50 15.0%), respectively. The results of the Spearman rank correlation analysis for EC 50 data versus the concentrations of toxicants in the sludge presented a significant correlation between toxicity and four heavy metals (Cd 2+ , Cu 2+ , Pb 2+ , Zn 2+ ). Introduction of the concept of toxicity unit (TU) showed that these metals were the main cause of toxicity in elutriates of at least four out of nine sludge samples. Members of the order Ulvales show a wide geographic distribution and have similar reproductive characteristics, thus making it possible to apply the present test method to other algae of this taxa, elsewhere. This novel method will be a useful tool for assessing the aquatic toxicity of a wide range of toxicants, once the

  9. A non-linear model for temperature-dependent sporulation and T-2 and HT-2 production of Fusarium langsethiae and Fusarium sporotrichioides.

    Science.gov (United States)

    Nazari, Leyla; Manstretta, Valentina; Rossi, Vittorio

    2016-04-01

    This research has produced new quantitative data on the sporulation and T-2+HT-2 toxin production that could be further integrated to develop a comprehensive disease or toxin prediction model for Fusarium langsethiae and Fusarium sporotrichioides. Experiments were conducted to determine the effect of temperature or incubation time on sporulation and the effect of temperature on T-2+HT-2 toxin production of strains of the two species. F. sporotrichioides demonstrated a preference for higher temperatures than F. langsethiae during sporulation; the optimum temperature was 24.5 ± 0.7 °C for F. langsethiae and 32.3 ± 2.1 °C for F. sporotrichioides, according to the Beta equation fitted to the data. The dynamics of sporulation over different incubation times were fitted by a Gompertz function. The maximum spore production was estimated to be after 18 and 8 d incubation at optimum temperatures for F. langsethiae and F. sporotrichioides, respectively. F. sporotrichioides produced more T-2+HT-2 than F. langsethiae. The best fit of the effect of temperature on T-2+HT-2 production in wheat grains was obtained with a Beta equation showing an optimum temperature of 14.7 ± 0.8 °C for F. langsethiae and 12.1 ± 0.2 °C for F. sporotrichioides. The optimum temperature for mycotoxin production was lower than for sporulation. Copyright © 2016 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

  10. Morphological and genetic characterization of group I Clostridium botulinum type B strain 111 and the transcriptional regulator spoIIID gene knockout mutant in sporulation.

    Science.gov (United States)

    Hosomi, Koji; Kuwana, Ritsuko; Takamatsu, Hiromu; Kohda, Tomoko; Kozaki, Shunji; Mukamoto, Masafumi

    2015-06-01

    Clostridium botulinum is a heat-resistant spore-forming bacterium that causes the serious paralytic illness botulism. Heat-resistant spores may cause food sanitation hazards and sporulation plays a central role in the survival of C. botulinum. We observed morphological changes and investigated the role of the transcriptional regulator SpoIIID in the sporulation of C. botulinum type B strain 111 in order to elucidate the molecular mechanism in C. botulinum. C. botulinum type B formed heat-resistant spores through successive morphological changes corresponding to those of Bacillus subtilis, a spore-forming model organism. An analysis of the spoIIID gene knockout mutant revealed that the transcriptional regulator SpoIIID contributed to heat-resistant spore formation by C. botulinum type B and activated the transcription of the sigK gene later during sporulation. Transcription of the spoIIID gene, which differed from that in B. subtilis and Clostridium difficile, was observed in the sigE gene knockout mutant of C. botulinum type B. An analysis of the sigF gene knockout mutant showed that the sporulation-specific sigma factor SigF was essential for transcription of the spoIIID gene in C. botulinum type B. These results suggest that the regulation of sporulation in C. botulinum is not similar to that in B. subtilis and other clostridia. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Emerging place image

    DEFF Research Database (Denmark)

    Ooi, Can-Seng; Peji´c Kristensen, Tatjana; Lomanová Pedersen, Zdenka

    2004-01-01

    and perceptions.This paper introduces the concept of the orientalist tourist gaze, and demonstrates howorientalism may manifest in tourism. Data on how these two countries are imagined werecollected in Denmark.Keywords: destination identity, host society-guest interaction, impact of tourism, orientalism......Tourism offers an arena through which a place identity is imagined, negotiated and contained.This paper compares the Czech Republic and Slovakia, and show how these countriesconstruct and assert their identities through tourism. They both share a common history asCzechoslovakia, however...

  12. Tourism Sociabilities and Place

    DEFF Research Database (Denmark)

    Bødker, Mads; Browning, David

    2013-01-01

    Proposing new design opportunities, this paper challenges received notions of tourism, arguing that tourism is fundamentally social and concerned with making place. This turn makes tourism not only a convenient testing ground for technology concepts, but increasingly also for more sensitive...... renderings of, and interventions in, tourism as a relational and social practice. Using examples from commercial, arts, and design projects, and providing excerpts from our own fieldwork and design workshops with tourists and locals, this paper outlines three challenges through a conceptual lens that we see...... as productive for appropriate interaction design of tourism technologies....

  13. Signs in Place

    DEFF Research Database (Denmark)

    Hamid, Salmiah Binti Abdul; Jensen, Ole B.; Silva, Victor

    Travelling in unfamiliar areas is usually very interesting, however it can also be stressful. People travel or move around in an urban space according to their needs, and the environment can also influence the way people move about from one place to another. If a person gets lost, a map or GPS can...... and geosemiotic studies with regards to the road traffic signs used in urban spaces. The paper ends with a discussion on how people choreograph their movement in their everyday life from two different perspectives: above vs. below....

  14. Signs In Place

    DEFF Research Database (Denmark)

    Hamid, Salmiah Binti Abdul; Jensen, Ole B.; Silva, Victor

    2012-01-01

    Travelling in unfamiliar areas is usually very interesting; however, it can also be stressful. People travel or move around in an urban space according to their needs, and the environment can influence the way people move about from one place to another. If a person gets lost, a map or GPS can...... and geosemiotic studies with regards to the road traffic signs used in urban spaces. The paper ends with a discussion on how people choreograph their movement in their everyday life from two different perspectives: above vs. below...

  15. Tackling the take-or-pay problem

    International Nuclear Information System (INIS)

    Jackson, Kim.

    1997-01-01

    Centrica, the gas sales, trading and services company previously part of British Gas plc, has renegotiated a number of its take-or-pay contracts with North Sea gas producers since the end of 1996. The contracts - a legacy of the British Gas monopoly era - had placed an increasing financial burden on the company as it was effectively forced to pay above-market prices for gas which it did not always want to take, while trying to remain competitive in a market where an ever growing number of independent gas suppliers were offering low-cost supplies. The author looks at how Centrica has tackled its take-or-pay problem. (author)

  16. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: C++ for Particle Physicists : 8 - 12.3.2004 (6 X 4-hour sessions) Introduction to the CERN EDMS : 9.3.2004 (1 day, free of charge) The EDMS MTF in Practice : 10.3.2004 (morning, free of charge) CLEAN-2002: Working in a Cleanroom : 10.3.2004 (afternoon, free of charge) The CERN EDMS for Engineers : 11.3.2004 (1 day, free of charge) LabVIEW hands-on (E):...

  17. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: LabVIEW base 1 : 25 - 27.2.2004(3 jours) Instructor-led WBTechT study or follow-up for Microsoft applications : 26.2.2004 (morning) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon - free of charge) C++ for Particle Physicists : 8 - 12.3.2004 (6 X 4-hour sessions) LabVIEW hands-on (E) : 16.3.2004 (afternoon) LabVIEW Basics 1 : 22 - 24.3.2004 ...

  18. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Instructor-led WBTechT study for Microsoft applications :12.2.2004 (morning) Instructor-led WBTechT study or follow-up for Microsoft applications : 19.2.2004 (morning) LabVIEW TestStand I (E) : 23 & 24.2.2004 (2 days) LabVIEW base 1 : 25 - 27.2.2004 (3 jours) Instructor-led WBTechT study or follow-up for Microsoft applications : 19.2.2004 (morning) CLEAN-2002 ...

  19. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Instructor-led WBTechT study or follow-up for Microsoft applications : 19.2.2004 (morning) LabVIEW TestStand I (E) : 23 & 24.2.2004 (2 days) LabVIEW base 1 : 25 - 27.2.2004 (3 jours) Instructor-led WBTechT study or follow-up for Microsoft applications : 26.2.2004 (morning) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon - free of charge) C++ for Pa...

  20. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Hands-on Object-Oriented Design and Programming with C++ : 22 - 24.3.2005 (3 days) FileMaker - niveau 2 : 4 & 5.4.2005 (2 jours) EMAG-2004 - Electromagnetic Design and Mathematical Optimization in Magnet Technology: 4- 14.4.2005 (8 x 3h) EXCEL 2003 - niveau 2 : 11 & 12.4.2005 (2 jours) LabVIEW Intermediate 1: 11 - 13.4.2005 (3 days) ACCESS 2003 - Level 2 - ECDL AM5: 13 & 14.4.2005 (2 days) LabVIEW Intermediate 2: 14 & 15.4.2005 (2 days) PowerPoint 2003 (F) : 18.4.2005 (1 jour) Joint PVSS JCOP Framework : 25 - 29.4.2005 (5 days) WORD 2003 - niveau 1 : 2 & 3.5.2005 (2 jours) ELEC-2005 - Summer Term: System electronics for physics - Issues : 10, 12, 17, 19, 24, 26 & 31.5.2005 (7 x 2h lectures) AutoCAD 2002 - niveau 1 : 11, 12, 18 & 19.5.2005 (4 jour...

  1. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Outlook (short course I) : E-mail : 31.8.2004 (2 hours, morning) Introduction à Outlook : Outlook (short course II) : Calendar, Tasks and Notes : 31.8.2004 (2 hours, afternoon) Instructor-led WBTechT Study or Follow-up for Microsoft Applications : 7.9.2004 (morning) Outlook (short course III) : Meetings and Delegation : 7.9.2004 (2 hours, afternoon) Introduction au VHDL et utilisation du simulateur ...

  2. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Joint PVSS JCOP Framework : 9 - 13.8.2004 (5 days) Introduction à Outlook : 19.8.2004 (1 jour) Outlook (Short Course I): E-mail: 31.8.2004 (2 hours, morning) Outlook (Short Course II): Calendar, Tasks and Notes: 31.8.2004 (2 hours, afternoon) Instructor-led WBTechT Study or Follow-up for Microsoft Applications: 7.9.2004 (morning) Outlook (Short Course III): Meetings and Delegation: 7.9.2004 (2 hours, afternoon) I...

  3. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Joint PVSS JCOP Framework : 9 - 13.8.2004 (5 days) Introduction à Outlook : 19.8.2004 (1 jour) Outlook (Short Course I): E-mail: 31.8.2004 (2 hours, morning) Outlook (Short Course II): Calendar, Tasks and Notes: 31.8.2004 (2 hours, afternoon) Hands-on Introduction to Python Programming: 1 - 3.9.2004 (3 days - free course) Instructor-led WBTechT Study or Follow-up for Microsoft Applications: 7.9.20...

  4. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Introduction à ANSYS : 23 - 26.11.2004 (4 jours) FileMaker - niveau 1 : 23 - 26.11.2004 (4 jours) Project Planning with MS-Project : 25.11 & 2.12.2004 (2 days) Explicit Dynamics with ANSYS/LS-Dyna : 7 - 9.12.2004 (3 days) Introduction to PERL 5 : 8 & 9.12.2004 (2 days) PCAD Schémas - Débutants : 9 & 10.12.2004 (2 jours) The JAVA Programming Language Level 1 : 11 & 12.1.2005 (2 days) Introduction to XML : 13 & 14.1.2005 (2 days) Introduction to the CERN EDMS : 18.1.2005 (1 day - free course) The CERN EDMS for Local Administrators : 19 & 20.1.2005 (2 days - free course) Programmation Unity-Pro pour utilisateurs de Schneider PL7-Pro : 24 - 28.1.2005 (8 demi-journées) CLEAN-2002 : Travaill...

  5. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Explicit Dynamics with ANSYS/LS-Dyna : 7 - 9.12.2004 (3 days) Introduction to PERL 5 : 8 & 9.12.2004 (2 days) PCAD Schémas - Débutants : 9 & 10.12.2004 (2 jours) Advanced aspects of PERL 5: 10.12.2004 (1 day) PCAD PCB – Débutants : 13 - 15.12.2004 (3 jours) The JAVA Programming Language Level 1 : 11 & 12.1.2005 (2 days) Introduction to XML : 13 & 14.1.2005 (2 days) Introduction to the CERN EDMS : 18.1.2005 (1 day - free course) The CERN EDMS for Local Administrators : 19 & 20.1.2005 (2 days - free course) Programmation Unity-Pro pour utilisateurs de Schneider PL7-Pro : 24 - 28.1.2005 (8 demi-journées) CLEAN-2002 : Travailler en salle propre : 25.1.2005 (après-midi, cours gratuit) Compatibilit&eac...

  6. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: FileMaker - niveau 1 : 23 - 26.11.2004 (4 jours) Explicit Dynamics with ANSYS/LS-Dyna : 7 - 9.12.2004 (3 days) Introduction to PERL 5 : 8 & 9.12.2004 (2 days) PCAD Schémas - Débutants : 9 & 10.12.2004 (2 jours) Advanced aspects of PERL 5: 10.12.2004 (1 day) PCAD PCB – Débutants : 13 - 15.12.2004 (3 jours) The JAVA Programming Language Level 1 : 11 & 12.1.2005 (2 days) Introduction to XML : 13 & 14.1.2005 (2 days) Introduction to the CERN EDMS : 18.1.2005 (1 day - free course) The CERN EDMS for Local Administrators : 19 & 20.1.2005 (2 days - free course) Programmation Unity-Pro pour utilisateurs de Schneider PL7-Pro : 24 - 28.1.2005 (8 demi-journées) CLEAN-2002 : Travailler en salle propre : 25.1.2005 ...

  7. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: C++ for Particle Physicists : 15 - 19.11.2004 (6 X 3 hours sessions) Word 2003 - niveau 1 : 22 & 23.11.2004 (2 jours) Introduction à ANSYS : 23 - 26.11.2004 (4 jours) Project Planning with MS-Project : 25.11 & 2.12.2004 (2 days) Explicit Dynamics with ANSYS/LS-Dyna : 7 - 9.12.2004 (2 days) PCAD Schémas - Débutants : 9 & 10.12.2004 (2 jours) The JAVA Programming Language Level 1 : 11 & 12.1.2005 (2 days) Introduction to XML : 13 & 14.1.2005 (2 days) CLEAN-2002 : Travailler en salle propre : 25.1.2005 (après-midi, cours gratuit) Compatibilité électromagnétique (CEM) : installation et remèdes : 25 - 27.1.2005 (3 jours) Finite State Machines in the JCOP Fr ...

  8. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: C++ for Particle Physicists : 8 - 12.3.2004 (6 X 4-hour sessions) Introduction to the CERN EDMS : 9.3.2004 (1 day, free of charge) The EDMS MTF in Practice : 10.3.2004 (morning, free of charge) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon, free of charge) The CERN EDMS for Engineers : 11.3.2004 (1 day, free of charge) LabVIEW...

  9. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: AutoCAD 2002 - niveau 1 : 13, 14, 23, 24.09.2004 (4 jours) Introduction to the CERN EDMS : 22.6.2004 (1 day) The CERN EDMS for local administrators : 23 & 24.6.2004 (2 days) MAGNE-04 : Magnétisme pour l'électrotechnique : 6 - 8.7.2004 (3 jours) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 7 & 8.9.2004 (2 jours) ENSEIGNEMENT TECHNIQUE TECHNICAL TRAINING...

  10. Technical Training: Places available

    CERN Document Server

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Joint PVSS JCOP Programming : 9 - 13.8.2004 (5 days) Hands-on Introduction to Python Programming: 1 - 3.9.2004 (3 days - free course) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 7 & 8.9.2004 (2 jours) Joint PVSS JCOP Programming : 13 - 17.9.2004 (5 days) AutoCAD 2002 - niveau 1 : 13, 14, 23, 24.9.2004 (4 jours) Programmation STEP7 niveau 1 : 14-17.9.2004 (4...

  11. Technical Training: Places available**

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The JAVA Programming Language Level 1 : 9 & 10.1.2004 (2 days) LabVIEW TestStand I (E) : 23 & 24.2.2004 (2 days) LabVIEW base 1 : 25 - 27.2.2004 (3 jours) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon - free of charge) C++ for Particle Physicists : 8 - 12.3.2004 ( 6 X 4-hour sessions) LabVIEW hands-on (E) 16.3...

  12. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Hands-on Introduction to Python Programming: 1 - 3.9.2004 (3 days - free course) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 7 & 8.9.2004 (2 jours) AutoCAD 2002 - niveau 1 : 13, 14, 23, 24.9.2004 (4 jours) Programmation STEP7 niveau 1 : 14-17.9.2004 (4 jours) FrontPage 2003 - niveau 1 : 20 & 21.9.2004 (2 jours) Word 2003 - niveau 2 : 27 & 28.9.2004 (2 jours) Introduction à Wind...

  13. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Introduction to the CERN EDMS : 22.6.2004 (1 day) The CERN EDMS for local administrators : 23 & 24.6.2004 (2 days) Compatibilité électromagnétique (CEM) - Introduction / Electromagnetic Compatibility (EMC) - Introduction: 7.7.2004 (morning) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 7 & 8.9.2004 (2 jours) AutoCAD 2002 - niveau 1 : 13, 14, 23, 24.9.2004 (4 jours) Programmation STEP...

  14. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: LabVIEW Intermediate I : 8 - 10.11.2004 (3 days) Instructor-led WTechT Study or Follow-up for Microsoft Applications : 9.11.2004 (morning) Hands-On Object Oriented Design and Programming with C++ : 9 - 11.11.2004 (3 days) Outlook (Short Course III) : Meetings and Delegation : 9.11.2004 (2 hours, afternoon) LabVIEW Intermediate II : 11 & 12.11.2004 (2 days) AutoCAD 2002 - niveau 1 : 11, 12, 18, 19.11.2004 (4 jours) C++ for Particle Physicists : 15 - 19.11.2004 (6 X 3 hours sessions) Word 2003 - niveau 1 : 22 & 23.11.2004 (2 jours) Introduction à ANSYS : 23 - 26.11.2004 (4 jours) CLEAN-2002 - Travailler en salle propre : 23.11.2004 (après-midi, cours gratuit) Project Planning with MS-Project : 25.11 & 2.12.2004 (2 days) ENSEIGNEMENT ...

  15. Technical training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: LabVIEW Intermediate I : 8 - 10.11.2004 (3 days) Instructor-led WTechT Study or Follow-up for Microsoft Applications : 9.11.2004 (morning) Outlook (Short Course III) : Meetings and Delegation : 9.11.2004 (2 hours, afternoon) LabVIEW Intermediate II : 11 & 12.11.2004 (2 days) AutoCAD 2002 - niveau 1 : 11, 12, 18, 19.11.2004 (4 jours) C++ for Particle Physicists : 15 - 19.11.2004 (6 X 3 hours sessions) Word 2003 - niveau 1 : 22 & 23.11.2004 (2 jours) Introduction à ANSYS : 23 - 26.11.2004 (4 jours) Project Planning with MS-Project : 25.11 & 2.12.2004 (2 days) Introduction to PERL 5 : 8 & 9.12.2004 (2 days) Advanced aspects of PERL 5 : 10.12.2004 (1 day) The JAVA Programming Language Level 1 : 11 & 12.1.2005 (2 days) Introduction...

  16. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Project Planning with MS-Project :6 & 13.5.2004 (2 days) Word 2003 - niveau 1 : 10 & 11.5.2004 (2 jours) Oracle 9i : SQL : 17 - 19.5.2004 (3 days) Word 2003 - niveau 2 : 24 & 25.5.2004 (2 jours) EXCEL 2003 - niveau 1: 27 & 28.5.2004 (2 jours) STEP7 Programming Level 1 : 1 - 4.6.2004 (4 days) Oracle 9i : Programming with PL/SQL : 2 - 4.6.2...

  17. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Finite State Machines in the JCOP Framework:  26 - 28.9.2005 (3 days) WORD 2003 (Short Course IV) - HowTo... Work with master document: 28.9.2005 (morning) Joint PVSS JCOP Framework : 3 - 7.10.2005 (5 days) Introduction à Dreamweaver MX :  3 - 4.10.2005 (2 jours) Utilisation des fichiers PDF avec Acrobat 7.0 :   4.10.2005 (1 journée) LaTeX par la pratique :   4 - 6.10.2005 (3 matinées) PowerPoint 2003 (F) :  7.10.2005 (1 journée) FileMaker - niveau 1 :  20 - 21.10.2005 (2 jours) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) WORD 2003 (Short Course I) - HowTo... Work with AutoTasks: 26.10.2005 (afternoon) OUTLOOK (Short Course I) - E-mail: 2.11.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 2.11....

  18. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    Le nombre de places disponibles peut varier. Veuillez consulter notre site Web pour avoir la dernière mise à jour. Des places sont disponibles dans les cours suivants :   Finite State Machines in the JCOP Framework:  26 - 28.9.2005 (3 days) MAGNE-05 - Magnétisme pour l'électrotechnique : 27 - 29.9.2005 (3 jours) WORD 2003 (Short Course IV) - HowTo... Work with master document: 28.9.2005 (morning) Joint PVSS JCOP Framework : 3 - 7.10.2005 (5 days) Introduction à Dreamweaver MX :  3 - 4.10.2005 (2 jours) Utilisation des fichiers PDF avec Acrobat 7.0 :   4.10.2005 (1 journée) LaTeX par la pratique :   4 - 6.10.2005 (3 matinées) PowerPoint 2003 (F) :  7.10.2005 (1 journée) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) WORD 2003 (Short Course I) - HowTo... Work with AutoTasks: 26.10.2005 (afternoon) OUTLOOK (Short Course I) - E-mail: 2.11.2005 (morning) WORD 2003 (...

  19. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Introduction à Windows XP au CERN : 12.9.2005 (1 demi-journée) FrontPage 2003 - niveau 2 : 15 - 16.9.2005 (2 jours) Finite State Machines in the JCOP Framework:  26 - 28.9.2005 (3 days) MAGNE-05 - Magnétisme pour l'électrotechnique : 27 - 29.9.2005 (3 jours) WORD 2003 (Short Course IV) - HowTo... Work with master document: 28.9.2005 (morning) Joint PVSS JCOP Framework : 3 - 7.10.2005 (5 days) Introduction à Dreamweaver MX :  3 - 4.10.2005 (2 jours) Utilisation des fichiers PDF avec Acrobat 7.0 :   4.10.2005 (1 journée) LaTeX par la pratique :   4 - 6.10.2005 (3 matinées) PowerPoint 2003 (F) :  7.10.2005 (1 journée) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) WORD 2003 (Short Course I) - HowTo... Work with Auto...

  20. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Joint PVSS JCOP Framework: 8 - 12.8.2005 (5 days) WORD 2003 (Short Course I) - HowTo... Work with AutoTasks (AutoText, AutoFormat, AutoCorrect, Lists, Find/Replace): 25.8.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 25.8.2005 (afternoon) EXCEL 2003 (Short Course I) - HowTo... Work with formulae: 26.8.2005 (morning) WORD 2003 (Short Course III) - HowTo... Work with long documents: 26.8.2005 (afternoon) FrontPage 2003 - niveau 1 : 1 - 2.9.2005 (2 jours) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.9.2005 (1 jour) LabVIEW Basics 1 : 5 - 7.9.2005 (3 days, dates to be confirmed) Introduction à Windows XP au CERN : 12.9.2005 (1 demi-journée) FrontPage 2003 - niveau 2 : 15 - 16.9.2005 (2 jours) AutoCAD 2005 - niveau 1 : 22, 23, 28, 29.9.2005 (4 jours) MAGNE-05 - Magn&...

  1. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.9.2005 (1 jour) LabVIEW Basics 1:  5 - 7.9.2005 (3 days) Introduction à Windows XP au CERN : 12.9.2005 (1 demi-journée) FrontPage 2003 - niveau 2 : 15 - 16.9.2005 (2 jours) AutoCAD 2005 - niveau 1 : 22, 23, 28, 29.9.2005 (4 jours) Finite State Machines in the JCOP Framework:  26 - 28.9.2005 (3 days) MAGNE-05 - Magnétisme pour l'électrotechnique : 27 - 29.9.2005 (3 jours) WORD 2003 (Short Course IV) - HowTo... Work with master document: 28.9.2005 (morning) Joint PVSS JCOP Framework : 3 - 7.10.2005 (5 days) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) WORD 2003 (Short Course I) - HowTo... Work with AutoTasks: 26.10.2005 (afternoon) OUTLOOK (Short Course I) - E-mail: 2...

  2. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Joint PVSS JCOP Framework: 8 - 12.8.2005 (5 days) WORD 2003 (Short Course I) - HowTo... Work with AutoTasks (AutoText, AutoFormat, AutoCorrect, Lists, Find/Replace): 25.8.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 25.8.2005 (afternoon) EXCEL 2003 (Short Course I) - HowTo... Work with formulae: 26.8.2005 (morning) WORD 2003 (Short Course III) - HowTo... Work with long documents: 26.8.2005 (afternoon) FrontPage 2003 - niveau 1 : 1 - 2.9.2005 (2 jours) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.9.2005 (1 jour) LabVIEW Basics 1 : 5 - 7.9.2005 (3 days, dates to be confirmed) Introduction à Windows XP au CERN : 12.9.2005 (1 demi-journée) FrontPage 2003 - niveau 2 : 15 - 16.9.2005 (2 jours) AutoCAD 2005 - niveau 1 : 22, 23, 28, 29.9.2005 (4 jours) Finite State Machines in the JCOP Framew...

  3. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Title Hours Date Language AutoCAD Mechanical 2006 16 30 to 31-01-06 F DIAdem : base 24 01 to 03-02-06 F ACROBAT 7.0 : Utilisation de fichiers PDF 8 06-02-06 F Manipulation des images 4 08-02-06 F OUTLOOK 2003 (Short Course I) - E-mail 3 09-02-06 E-F WORD 2003 (Short Course II) - HowTo... Mail merge 3 09-02-06 E-F ACCESS 2003 - Level 1: ECDL M5 16 13 to 14-02-06 E-F JCOP: Control System Integration using JCOP Tools 24 14 to 16-02-06 E OUTLOOK 2003 (Short Course II) - Calendar, Tasks and Notes 3 16-02-06 E-F WORD 2003 (Short Course III) - HowTo... Work with long documents 3 16-02-06 E-F FrontPage 2003 - niveau 1 16 23 to 24-02-06 F OUTLOOK 2003 (Short Course III) - Meetings and Delegation 3 27-02-06 E-F WORD 2003 (Short Course IV) - HowTo... Work with master document 3 27-02-06 E-F ...

  4. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2006-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Title Hours Date Language ACCESS 2003 - Level 2: ECDL AM5 16 19 to 20-01-06 E-F AutoCAD 2006 - niveau 1 32 19 to 25-01-06 F C++ Programming Advanced - Traps and Pitfalls 32 24 to 27-01-06 E STEP7 : Level 1 32 24 to 27-01-06 E LabVIEW Basics 2 16 26 to 27-01-06 E AutoCAD Mechanical 2006 16 30 to 31-01-06 F DIAdem : base 24 01 to 03-02-06 F FrontPage 2003 - niveau 1 16 02 to 03-02-06 F ACROBAT 7.0 : Utilisation de fichiers PDF 8 06-02-06 F Manipulation des images 4 08-02-06 F OUTLOOK 2003 (Short Course I) - E-mail 3 09-02-06 E-F WORD 2003 (Short Course II) - HowTo... Mail merge 3 09-02-06 E-F ACCESS 2003 - Level 1: ECDL M5 16 13 to 14-02-06 E-F JCOP: Control System Integration using JCOP Tools 24 14 to 16-02-06 E OUTLOOK 2003 (Short Course II) - Calendar, Tasks and Note...

  5. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: WORD 2003 (Short Course I) - HowTo... Work with AutoTasks (AutoText, AutoFormat, AutoCorrect, Lists, Find/Replace): 25.8.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 25.8.2005 (afternoon) EXCEL 2003 (Short Course I) - HowTo... Work with formulae: 26.8.2005 (morning) WORD 2003 (Short Course III) - HowTo... Work with long documents: 26.8.2005 (afternoon) FrontPage 2003 - niveau 1 : 1 - 2.9.2005 (2 jours) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.9.2005 (1 jour) LabVIEW base 1 : 5 - 7.9.2005 (3 jours) Introduction à Windows XP au CERN : 12.9.2005 (1 demi-journée) FrontPage 2003 - niveau 2 : 15 - 16.9.2005 (2 jours) AutoCAD 2005 - niveau 1 : 22, 23, 28, 29.9.2005 (4 jours) Finite State Machines in the JCOP Framework:  26 - 28.9.2005 (3 days) MAGNE-05 - Magnétisme pour l'électrotechniq...

  6. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: LabVIEW Real-Time (F) : 7 - 9.6.2005 (3 jours) LabVIEW Migration 6 to 7: 14.6.2005 (1 day) FrontPage 2003 - niveau 2 : 16 & 17.6.2005 (2 jours) Utilisation de fichiers PDF avec ACROBAT 6.0 : 20.6.2005 (1journée) Introduction to ANSYS: 21 - 24.6.2005 (4 days) WORD 2003 (Short Course I) - HowTo... Work with repetitive tasks /AutoText, AutoFormat, AutoCorrect, Find/Replace) : 4.7.2005 (afternoon) WORD 2003 (Short Course II) - HowTo... Mail merge: 5.7.2005 (afternoon) WORD 2003 (Short Course III) - HowTo... Work with long documents : 6.7.2005 (afternoon) ACCESS 2003 - Level 2 - ECDL AM5: 5 - 8.7.2005 (4 mornings) EXCEL 2003 (Short Course I) - HowTo... Work with formulae: 7.7.2005 (afternoon) EXCEL 2003 (Short Course II) - HowTo... Format your worksheet for printing: 8.7.2005 (aftern...

  7. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: LabVIEW base 1 : 4 - 6.7.2005 (3 jours) LabVIEW Basics 2: 7 - 8.7.2005 (2 days) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.7.2005 (1 jour) FrontPage 2003 - niveau 1 : 6-7.7.2005 (2 jours) WORD 2003 (Short Course I) - HowTo... Work with repetitive tasks /AutoText, AutoFormat, AutoCorrect, Find/Replace) : 4.7.2005 (afternoon) WORD 2003 (Short Course II) - HowTo... Mail merge: 5.7.2005 (afternoon) WORD 2003 (Short Course III) - HowTo... Work with long documents : 6.7.2005 (afternoon) OUTLOOK (Short Course I) - E-mail: 6.7.2005 (morning) OUTLOOK (Short Course II) - Calendar, Tasks and Notes: 7.7.2005 (morning) OUTLOOK (Short Course III) - Meetings and Delegation: 8.7.2005 (morning) EXCEL 2003 (Short Course I) - HowTo... Work with formulae: 7.7.2005 (afternoon) EXCEL 2003 (Short Course II) - HowTo... Format y...

  8. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: LabVIEW Real-Time (F) : 7 - 9.6.2005 (3 jours) LabVIEW Migration 6 to 7: 14.6.2005 (1 day) IT3T/1 - Read your mail and more with Outlook 2003 : 14.6.2005 (IT Technical Training Tutorial, free of charge) IT3T/2 - Creating, managing and using distribution lists with Simba2 : 16.6.2005 (IT Technical Training Tutorial, free of charge) FrontPage 2003 - niveau 2 : 16 & 17.6.2005 (2 jours) Utilisation de fichiers PDF avec ACROBAT 6.0 : 20.6.2005 (1journée) Introduction to ANSYS: 21 - 24.6.2005 (4 days) IT3T/3 - Working remotely with Windows XP : 28.6.2005 (IT Technical Training Tutorial, free of charge) IT3T/4 - Editing Websites with Frontpage 2003 : 30.6.2005 (IT Technical Training Tutorial, free of charge) WORD 2003 (Short Course I) - HowTo... Work with repetitive tasks /AutoText, AutoFormat, AutoC...

  9. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Hands-on Introduction to Python Programming: 28 - 30.6.2005 (3 days) Introduction to ANSYS: 28.6 - 1.7.2005 (4 days) IT3T/3 - Working remotely with Windows XP: 28.6.2005 (IT Technical Training Tutorial, free of charge) IT3T/4 - Editing Websites with Frontpage 2003: 30.6.2005 (IT Technical Training Tutorial, free of charge) LabVIEW base 1 : 4 - 6.7.2005 (3 jours) LabVIEW Basics 2: 7 - 8.7.2005 (2 days) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.7.2005 (1 jour) FrontPage 2003 - niveau 1 : 6-7.7.2005 (2 jours) WORD 2003 (Short Course I) - HowTo... Work with repetitive tasks /AutoText, AutoFormat, AutoCorrect, Find/Replace) : 4.7.2005 (afternoon) WORD 2003 (Short Course II) - HowTo... Mail merge: 5.7.2005 (afternoon) WORD 2003 (Short Course III) - HowTo... Work with long documents : 6.7.2005 (afternoon) ACCES...

  10. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Joint PVSS JCOP Framework: 8 - 12.8.2005 (5 days) FrontPage 2003 - niveau 1 : 1 - 2.9.2005 (2 jours) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.9.2005 (1 jour) LabVIEW Basics 1 : 5 - 7.9.2005 (3 days, dates to be confirmed) Introduction à Windows XP au CERN : 12.9.2005 (1 demi-journée) FrontPage 2003 - niveau 2 : 15 - 16.9.2005 (2 jours) AutoCAD 2005 - niveau 1 : 22, 23, 28, 29.9.2005 (4 jours) MAGNE-05 - Magnétisme pour l'électrotechnique : 27 - 29.9.2005 (3 jours) LabVIEW Application Development (Intermediate I): 5 - 7.12.2005 (3 days) LabVIEW Advanced Programming (Intermediate 2): 8 - 9.12.2005 (2 days) ENSEIGNEMENT TECHNIQUE TECHNICAL TRAINING Monique Duval 74924 technical.training@cern.ch

  11. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The JAVA Programming Language Level 1: 11 & 12.1.2005 (2 days) Introduction to XML : 13 & 14.1.2005 (2 days) The CERN EDMS for Local Administrators: 19 & 20.1.2005 (2 days - free course) Programmation Unity-Pro pour utilisateurs de Schneider PL7-Pro : 24 - 28.1.2005 (8 demi-journées) LabVIEW base 1/basics 1 : 31.1 - 1.2.2005 (2 j size="2">LabVIEW base 2/basics 2 : 3 & 4.2.2005 (2 jours/2 days - langue à décider/language to be decided) Finite State Machines in the JCOP Framework: 1 - 3.2.2005 (3 days - free course) The JAVA Programming Language Level 2: 7 - 9.2.2005 (3 days) C++ Programming Advanced -Traps and Pitfalls: 8 - 11.2.2005 (4 days) Joint PVSS JCOP Framework: 14 - 18.2.2005 (5 days) JAVA 2 Enterprise Edition - Part 1: ...

  12. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Joint PVSS JCOP Framework : 3 - 7.10.2005 (5 days) Utilisation des fichiers PDF avec Acrobat 7.0 :   4.10.2005 (1 journée) LaTeX par la pratique :   4 - 6.10.2005 (3 matinées) PowerPoint 2003 (F) :  7.10.2005 (1 journée) FileMaker - niveau 1 :  20 - 21.10.2005 (2 jours) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) Finite State Machines in the JCOP Framework : 25 - 27.10.2005 (3 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) WORD 2003 (Short Course I) - HowTo... Work with AutoTasks: 26.10.2005 (afternoon) OUTLOOK (Short Course I) - E-mail: 2.11.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 2.11.2005 (afternoon) FrontPage 2003 - niveau 1 :  3 - 4.11.2005 (2 jours) AutoCAD 2006 - niveau 1 : 3, 4, 9, 10.11.2005 (4 jours) Joint PVSS JCO...

  13. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: LabVIEW Intermediate I : 8 - 10.11.2004 (3 days) Instructor-led WTechT Study or Follow-up for Microsoft Applications : 9.11.2004 (morning) Hands-On Object Oriented Design and Programming with C++ : 9 - 11.11.2004 (3 days) Outlook (Short Course III) : Meetings and Delegation : 9.11.2004 (2 hours, afternoon) LabVIEW Intermediate II : 11 & 12.11.2004 (2 days) AutoCAD 2002 - niveau 1 : 11, 12, 18, 19.11.2004 (4 jours) C++ for Particle Physicists : 15 - 19.11.2004 (6 X 3 hours sessions) Word 2003 - niveau 1 : 22 & 23.11.2004 (2 jours) Introduction à ANSYS : 23 - 26.11.2004 (4 jours) CLEAN-2002 - Travailler en salle propre : 23.11.2004 (après-midi, cours gratuit) Project Planning with MS-Project : 25.11 & 2.12.2004 (2 days) PCAD Sch&eac...

  14. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: FileMaker - niveau 1 :  20 - 21.10.2005 (2 jours) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) Finite State Machines in the JCOP Framework : 25 - 27.10.2005 (3 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) WORD 2003 (Short Course I) - HowTo... Work with AutoTasks: 26.10.2005 (afternoon) OUTLOOK (Short Course I) - E-mail: 2.11.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 2.11.2005 (afternoon) FrontPage 2003 - niveau 1 :  3 - 4.11.2005 (2 jours) AutoCAD 2006 - niveau 1 : 3, 4, 9, 10.11.2005 (4 jours) Joint PVSS JCOP Framework : 7 - 11.11.2005 (5 days) OUTLOOK (Short Course II) - Calendar, Tasks and Notes: 16.11.2005 (morning) Joint PVSS JCOP Framework : 21 - 25.11.2005 (5 days) OUTLOOK (Short Course III) - Meetings and Delegation: 30.11....

  15. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: FileMaker - niveau 1 :  20 - 21.10.2005 (2 jours) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) Finite State Machines in the JCOP Framework : 25 - 27.10.2005 (3 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) OUTLOOK (Short Course I) - E-mail: 2.11.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 2.11.2005 (afternoon) FrontPage 2003 - niveau 1 :  3 - 4.11.2005 (2 jours) AutoCAD 2006 - niveau 1 : 3, 4, 9, 10.11.2005 (4 jours) Joint PVSS JCOP Framework : 7 - 11.11.2005 (5 days) OUTLOOK (Short Course II) - Calendar, Tasks and Notes: 16.11.2005 (morning) Joint PVSS JCOP Framework : 21 - 25.11.2005 (5 days) OUTLOOK (Short Course III) - Meetings and Delegation: 30.11.2005 (morning) WORD 2003 (Short Course III) - HowTo... Work with long documents : ...

  16. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: FrontPage 2003 - niveau 1 : 1 - 2.9.2005 (2 jours) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.9.2005 (1 jour) LabVIEW Basics 1:  5 â€" 7.9.2005 (3 days) Introduction à Windows XP au CERN : 12.9.2005 (1 demi-journée) FrontPage 2003 - niveau 2 : 15 - 16.9.2005 (2 jours) AutoCAD 2005 - niveau 1 : 22, 23, 28, 29.9.2005 (4 jours) Finite State Machines in the JCOP Framework:  26 - 28.9.2005 (3 days) MAGNE-05 - Magnétisme pour l'électrotechnique : 27 - 29.9.2005 (3 jours) Joint PVSS JCOP Framework : 3 - 7.10.2005 (5 days) ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) OUTLOOK (Short Course I) - E-mail: 2.11.2005 (morning) OUTLOOK (Short Course II) - Calendar, Tasks and Notes: 16.11.2005 (morning) OU...

  17. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The JAVA Programming Language Level 1: 11 & 12.1.2005 (2 days) Introduction to XML : 13 & 14.1.2005 (2 days) ELEC-2005.:Winter term: Introduction to electronics in HEP: 18, 20, 25, 27.1, 1 & 3.2.2005 (6 x 2h lectures) The CERN EDMS for Local Administrators: 19 & 20.1.2005 (2 days - free course) Programmation Unity-Pro pour utilisateurs de Schneider PL7-Pro : 24 - 28.1.2005 (8 demi-journées) CLEAN-2002 : Travailler en salle propre : 25.1.2005 (après-midi, cours gratuit) Compatibilité électromagnétique (CEM) : installation et remèdes : 25 - 27.1.2005 (3 jours) Finite State Machines in the JCOP Framework: 1 - 3.2.2005 (3 days - free course) The JAVA Programming Language Level 2: 7 - 9...

  18. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: The CERN EDMS for Local Administrators: 19 & 20.1.2005 (2 days - free course) Programmation Unity-Pro pour utilisateurs de Schneider PL7-Pro : 24 - 28.1.2005 (9 demi-journées) LabVIEW base 1/basics 1 : 31.1 - 2.2.2005 (3 jours/3 days - langue à décider/language to be decided) LabVIEW base 2/basics 2 : 3 & 4.2.2005 (2 jours/2 days - langue à décider/language to be decided) Finite State Machines in the JCOP Framework: 1 - 3.2.2005 (3 days - free course) The JAVA Programming Language Level 2: 7 - 9.2.2005 (3 days) C++ Programming Advanced -Traps and Pitfalls: 8 - 11.2.2005 (4 days) Joint PVSS JCOP Framework: 14 - 18.2.2005 (5 days) JAVA 2 Enterprise Edition - Part 1: WEB Applications: 21 & 22.2.2005 (2 days) JAVA 2 Enterprise Edition - Part 2: Enterprise JavaBeans: 23 - 25.2.2005 (3 days) ELEC-2005 â...

  19. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: LabVIEW base 1/basics 1 : 31.1 - 2.2.2005 (3 jours/3 days - langue à décider/language to be decided) LabVIEW base 2/basics 2 : 3 & 4.2.2005 (2 jours/2 days - langue à décider/language to be decided) Finite State Machines in the JCOP Framework: 1 - 3.2.2005 (3 days - free course) The JAVA Programming Language Level 2: 7 - 9.2.2005 (3 days) C++ Programming Advanced -Traps and Pitfalls: 8 - 11.2.2005 (4 days) Joint PVSS JCOP Framework: 14 - 18.2.2005 (5 days) JAVA 2 Enterprise Edition - Part 1: WEB Applications: 21 & 22.2.2005 (2 days) FrontPage 2003 - niveau 1 : 21 & 22.2.2005 (2 jours) JAVA 2 Enterprise Edition - Part 2: Enterprise JavaBeans: 23 - 25.2.2005 (3 days) ELEC-2005 - Spring Term: Integrated circuits and VLSI technology...

  20. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Joint PVSS JCOP Framework : 9 - 13.8.2004 (5 days) Outlook (I): E-mail : 31.8.2004 (2 hours, morning) Outlook (II): Calendar, Tasks and Notes : 31.8.2004 (2 hours, afternoon) Hands-on Introduction to Python Programming : 1 - 3.9.2004 (3 days - free course) Instructor-led WBTechT Study or Follow-up for Microsoft Applications : 7.9.2004 (morning) Outlook (III): Meetings and Delegation : 7.9.2004 (2 hours, afternoon) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 7 & 8.9.2004 (2 jours) Joint PVSS JCOP Framework : 13 - 17.9.2004 (5 days) AutoCAD 2002 - niveau 1 : 13, 14, 23, 24.9.2004 (4 jours) Programmation STEP7 niveau 1 : 14 - 17.9.2004 (4 jours) FrontPage 2003 - niveau 1 : 20 & 21.9.2004 (2 jours) ANSYS: Thermal Analysis : 22 - 24.9.2004 (3 days) ANSYS: Advanced Topics : 27.9...

  1. Technical Training: Places available

    CERN Document Server

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Instructor-led WBTechT Study or Follow-up for Microsoft Applications : 7.9.2004 7.9.2004 Outlook (short course III) : Meetings and Delegation : 7.9.2004 (2 hours, afternoon) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 7 & 8.9.2004 (2 jours) Joint PVSS JCOP Framework : 13 - 17.9.2004 (5 days) Programmation STEP7 niveau 1 : 14-17.9.2004 (4 jours) ANSYS : Thermal Analysis : 22 - 24.9.2004 (3 days) LabVIEW Migration 6 à 7 : 23.9.2004 (one day) ANSYS : Advanced Topics : 27.9 - 1.10.2004 (5 days) Word 2003 - niveau 2 : 27 & 28.9.2004 (2 jours) LabVIEW - Basics 1 : 27 - 29.9.2004 (3 days) MAGNE-04 : Magnétisme pour l'électrotechnique : 28 - 30.9.2004 (3 jours) LabVIEW - Basics 2 : 30.9 & 1.10.2004 (2 days) Introduction à Windows XP au CERN : 4.10.2004 (matin) ...

  2. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Programmation STEP7 niveau 1 : 14-17.9.2004 (4 jours) ANSYS : Thermal Analysis : 22 - 24.9.2004 (3 days) LabVIEW Migration 6 à 7 : 23.9.2004 (one day) ANSYS : Advanced Topics : 27.9 - 1.10.2004 (5 days) Word 2003 - niveau 2 : 27 & 28.9.2004 (2 jours) LabVIEW - Basics 1 : 27 - 29.9.2004 (3 days) MAGNE-04 : Magnétisme pour l'électrotechnique : 28 - 30.9.2004 (3 jours) LabVIEW - Basics 2 : 30.9 & 1.10.2004 (2 days) Introduction à Windows XP au CERN : 4.10.2004 (matin) FrontPage 2003 - niveau 1 : 7 & 8.10.04 (2 jours) Outlook (short course I) : E-mail : 22.10.2004 (2 hours, morning) Outlook (short course II) : Calendar, Tasks and Notes: 22.10.2004 (2 hours, afternoon) Introduction à ANSYS : 23 - 26.11.2004 (4 jours) ENSEIGNEMENT TECHNIQUE TECHNICAL TRAINING Monique Duval 74924...

  3. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Hands-on Introduction to Python Programming: 28 - 30.6.2005 (3 days) Introduction to ANSYS: 21 - 24.6.2005 (4 days) IT3T/3 - Working remotely with Windows XP: 28.6.2005 (IT Technical Training Tutorial, free of charge) IT3T/4 - Editing Websites with Frontpage 2003: 30.6.2005 (IT Technical Training Tutorial, free of charge) Utilisation des fichiers PDF avec ACROBAT 7.0 : 5.7.2005 (1 jour) FrontPage 2003 - niveau 1 : 6-7.7.2005 (2 jours) LabVIEW base 1 : 4 - 6.7.2005 (3 jours) LabVIEW Basics 2: 7 - 8.7.2005 (2 days) WORD 2003 (Short Course I) - HowTo... Work with repetitive tasks /AutoText, AutoFormat, AutoCorrect, Find/Replace) : 4.7.2005 (afternoon) WORD 2003 (Short Course II) - HowTo... Mail merge: 5.7.2005 (afternoon) WORD 2003 (Short Course III) - HowTo (4 mornings) EXCEL 2003 (Short Course I) - HowTo... Work ...

  4. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Oracle 9i : New features for developers : 14 - 16.6.2004 (3 days) The Joint PVSS JCOP Framework: 14 - 18.6.2004 (5 days) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 15 & 16.6.2004 (2 jours) EXCEL 2003 - niveau 2 : 17 & 18.6.2004 (2 jours) MAGNE-04 : Magnétisme pour l'électrotechnique : 6 au 8.7.2004 (3 jours) AutoCAD 2002 - niveau 1 : 13, 14, 23, 24.09.2004 (4 jours) ENSEIGNEMEN...

  5. Technical Training: Places Available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval - Tel.74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Oracle 9i: SQL: 17 - 19.5.2004 (3 days) Word 2003 - niveau 2 : 24 & 25.5.2004 (2 jours) EXCEL 2003 - niveau 1 : 27 & 28.5.2004 (2 jours) STEP7 Programming Level 1: 1 - 4.6.2004 (4 days) Oracle 9i : Programming with PL/SQL: 2 - 4.6.2004 (3 days) CST Microwave Studio: 3 & 4.6.2004 (2 days) Oracle 9i : New f...

  6. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval - Tel.74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Word 2003 - niveau 2 : 24 & 25.5.2004 (2 jours) VisualEliteHDL : 25 & 26.5.2004 (2 days) EXCEL 2003 - niveau 1 : 27 & 28.5.2004 (2 jours) STEP7 Programming Level 1: 1 - 4.6.2004 (4 days) Oracle 9i : Programming with PL/SQL: 2 - 4.6.2004 (3 days) CST Microwave Studio: 3 & 4.6.2004 (2 days) Oracle 9...

  7. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: Utilisation des fichiers PDF avec ACROBAT 6.0 : 1.3.2005 (1 journée) ELEC-2005 - Spring Term: Integrated circuits and VLSI technology for physics: 1 - 17.3.2005 (6 x 2.5-hour lectures) C++ for Particle Physicists: 7 - 11.3.2005 (6 x 3-hour lectures) Joint PVSS JCOP Framework : 14 - 18.3.2005 (5 days) AXEL-2005; Introduction to Particle Accelerators : 14- 18.3.2005 (10 x 1 h lectures) ACCESS 2003 - Level 1: ECDL M5: 15 - 16.3.2005 (2 days) AutoCAD 2002 - niveau 1 : 15, 16, 21& 22.3.2005 (4 jours) FrontPage 2003 - niveau 2 : 21 & 22.3.2005 (2 jours) Hands-on Object-Oriented Design and Programming with C++ : 22 - 24.3.2005 (3 days) FileMaker - niveau 2 : 4 & 5.4.2005 (2 jours) EXCEL 2003 - niveau 2 : 11 & 12.4.2005 (2 jours) LabVIEW Intermediate 1: 11 - 13.4.2005...

  8. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: JAVA 2 Enterprise Edition - Part 1: WEB Applications: 21 & 22.2.2005 (2 days) FrontPage 2003 - niveau 1 : 21 & 22.2.2005 (2 jours) JAVA 2 Enterprise Edition - Part 2: Enterprise JavaBeans: 23 - 25.2.2005 (3 days) Utilisation des fichiers PDF avec ACROBAT 6.0 : 1.3.2005 (1 journée) Hands-on Object-Oriented Design and Programming with C++ : 1 - 3.3.2005 (3 days) ELEC-2005 - Spring Term: Integrated circuits and VLSI technology for physics: 1 - 17.3.2005 (6 x 2.5-hour lectures) C++ for Particle Physicists: 7 - 11.3.2005 (6 x 3-hour lectures) Joint PVSS JCOP Framework : 14 - 18.3.2005 (5 days) ACCESS 2003 - Level 1 : 15 - 16.3.2005 (2 days) AutoCAD 2002 - niveau 1 : 15, 16, 21& 22.3.2005 (4 jours) FrontPage 2003 - niveau 2 : 21 & 22.3.2005 (2 jours) FileMaker - ...

  9. THE PLACES FOR PARTICIPATION

    Directory of Open Access Journals (Sweden)

    Tania Carson

    2007-09-01

    Full Text Available The current upsurge of interest in street art with its direct approach to the city emphasizes the need to readdress public arts’ role within the wider social and cultural context. As it becomes routine to incorporate art works into city planning and city life, art has become both ‘safe’ and ‘user‐friendly’. On the one hand, art is subsumed and immersed into the wider culture through leisure and entertainment policies, socially engaged public art projects, community art and urban design. On the other hand, there are those works which deliberately intend to unsettle or confront the viewer. In recent years the term intervention has been ascribed to unorthodox art works in the public sphere which are often illicit, or at least non‐complicit in the prevailing hegemony. These practices are disruptive due to their enactment outside traditional art spaces, often in the streets. By occupying the streets, places of democracy and disjunction, these interventions transform public space back into a place of overt cultural and political expression. They also deliberately involve the inhabitants of the city by utilizing everyday spaces in ways which offer an unmediated sense of the artwork.

  10. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: FrontPage 2003 - niveau 1 : 9 & 10.5.2005 (2 jours) ELEC-2005 - Summer Term: System electronics for physics - Issues : 10, 12, 17, 19, 24, 26 & 31.5.2005 (7 x 2h lectures) AutoCAD 2005 - niveau 1 : 12, 13, 18, 19.5.2005 (4 jours) ACCESS 2003 - Level 1: ECDL M5 : 11 & 12.5.2005 (2 days) Object-Oriented Analysis and Design using UML: 17 - 19.5.2005 (3 days) Synplify Pro Training: 18.5.2005 (1 day) Finite State Machines in the JCOP Framework: 24 - 26.5.2005 (3 days) The Joint PVSS JCOP Framework: 30.5 - 3.6.2005 (5 days) Introduction à la CAO CADENCE : 31.5 - 1.6.2005 (2 jours) LabVIEW Real-Time (F) : 7 - 9.6.2005 (3 jours) LabVIEW Migration 6 to 7: 14.6.2005 (1 day) FrontPage 2003 - niveau 2 : 16 & 17.6.2005 (2 jours) Utilisation de fichiers PDF avec ACROBAT 6.0 : 20.6.2005 (1journée) Intr...

  11. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: FrontPage 2003 - niveau 1 : 9 & 10.5.2005 (2 jours) ELEC-2005 - Summer Term: System electronics for physics - Issues : 10, 12, 17, 19, 24, 26 & 31.5.2005 (7 x 2h lectures) AutoCAD 2005 - niveau 1 : 11, 12, 18, 19.5.2005 (4 jours) Object-Oriented Analysis and Design using UML: 17 - 19.5.2005 (3 days) Synplify Pro Training: 18.5.2005 (1 day) Finite State Machines in the JCOP Framework: 24 - 26.5.2005 (3 days) The Joint PVSS JCOP Framework: 30.5 - 3.6.2005 (5 days) Introduction à la CAO CADENCE : 31.5 - 1.6.2005 (2 jours) LabVIEW Real-Time (F) : 7 - 9.6.2005 (3 jours) LabVIEW Migration 6 to 7: 14.6.2005 (1 day) FrontPage 2003 - niveau 2 : 16 & 17.6.2005 (2 jours) Utilisation de fichiers PDF avec ACROBAT 6.0 : 20.6.2005 (1journée) Introductio...

  12. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: The Java Programming Language Level 1: 21 - 23.11.2005 (3 days) OUTLOOK (Short Course III) - Meetings and Delegation: 30.11.2005 (morning) WORD 2003 (Short Course III) - HowTo... Work with long documents : 30.11.2005 (afternoon) FrontPage 2003 - niveau 2 : 5 - 6.12.2005 (2 jours) Introduction à ANSYS Classique : 6 - 9.12.2005 (4 jours) EXCEL 2003 (Short Course II) - HowTo... Format your worksheet for printing: 7.12.2005 (morning) PCAD Schémas - Introduction : 8 - 9.12.2005 (2 jours) ACCESS 2003 - Level 2 - ECDL AM5: 14 - 15.12.2005 (2 days) LabVIEW Basics I:  23 - 25.1.2006 (3 days) C++ Programming Advanced - Traps and Pitfalls: 24 - 27.1.2006 (4 days) LabVIEW Basics II:  26 - 27.1.2006 (2 days) Joint PVSS-JCOP Framework: 30.1 - 3.2.2006 (5 days, free of charge) Finite State Machines in the JCOP Framework : ...

  13. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: FrontPage 2003 - niveau 2 : 5 - 6.12.2005 (2 jours) Introduction à ANSYS Classique : 6 - 9.12.2005 (4 jours) EXCEL 2003 (Short Course II) - HowTo... Format your worksheet for printing: 7.12.2005 (morning) PCAD Schémas - Introduction : 8 - 9.12.2005 (2 jours) Finite State Machines in the JCOP Framework: 13 - 15.12.2005 (3 days) ACCESS 2003 - Level 2 - ECDL AM5: 14 - 15.12.2005 (2 days) PCAD PCB - Introduction : 14 - 16.12.2005 (3 jours) AutoCAD 2006 - niveau 1 : 19, 20, 24 & 25.1.2006 (4 jours) LabVIEW Basics I:  23 - 25.1.2006 (3 days) C++ Programming Advanced - Traps and Pitfalls: 24 - 27.1.2006 (4 days) STEP7 Programming Level 1: 24 - 27.1.2006 (4 days) LabVIEW Basics II:  26 - 27.1.2006 (2 days) AutoCAD Mechanical 2006 : 30 - 31.1.2006 (2 jours; suite du cours AutoCAD 2006 - niveau 1) Joint PVS...

  14. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: ACCESS 2003 - Level 1 - ECDL M5: 24 - 25.10.2005 (2 days) Finite State Machines in the JCOP Framework : 25 - 27.10.2005 (3 days) EXCEL 2003 (Short Course III) - HowTo... Pivot tables: 26.10.2005 (morning) Introduction to the CERN EDMS: 2.11.2005 (1 day, free of charge) OUTLOOK (Short Course I) - E-mail: 2.11.2005 (morning) WORD 2003 (Short Course II) - HowTo... Mail merge: 2.11.2005 (afternoon) FrontPage 2003 - niveau 1 :  3 - 4.11.2005 (2 jours) AutoCAD 2006 - niveau 1 : 3, 4, 9, 10.11.2005 (4 jours) Joint PVSS JCOP Framework : 7 - 11.11.2005 (5 days) The CERN EDMS for Engineers: 8.11.2005 (1 day, free of charge) The EDMS-MTF in practice: 9.11.2005 (morning, free of charge) The CERN EDMS for Local Administrators: 15-16.11.2005 (2 days, free of charge) OUTLOOK (Short Course II) - Calendar, Tasks and Notes: ...

  15. Technical Training: Places available

    CERN Multimedia

    Davide Vitè

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: OUTLOOK (Short Course III) - Meetings and Delegation: 30.11.2005 (morning) WORD 2003 (Short Course III) - HowTo... Work with long documents: 30.11.2005 (afternoon) FrontPage 2003 - niveau 2 : 5 - 6.12.2005 (2 jours) Introduction à ANSYS Classique : 6 - 9.12.2005 (4 jours) EXCEL 2003 (Short Course II) - HowTo... Format your worksheet for printing: 7.12.2005 (morning) AutoCAD - mise à jour d’AutoCAD 2002 à AutoCAD 2006 : 8.12.2005 (1 journée - pour les utilisateurs d’AutoCAD 2002) PCAD Schémas - Introduction : 8 - 9.12.2005 (2 jours) Finite State Machines in the JCOP Framework: 13 - 15.12.2005 (3 days) PCAD PCB - Introduction : 14 - 16.12.2005 (3 jours) ACCESS 2003 - Level 2 - ECDL AM5: 14 - 15.12.2005 (2 days) AutoCAD 2006 - niveau 1 : 19, 20, 24 & 25.1.2006 (4 jours) LabVIEW Basics I:  23 - 25.1.2006...

  16. TECHNICAL TRAINING: PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2004-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: LabVIEW Basics 1 : 22 - 24.3.2004 (3 days) Oracle 9i : New Features for Developers : 22 - 24.3.2004 (3 days) Instructor-led WBTechT study or follow-up for Microsoft applications : 1.4.2004 (morning) FrontPage XP - niveau 1 : 5 & 6.4.2004 (2 jours) AutoCAD 2002 - niveau 1 : 19, 20.4 et 3, 4.5.2004 (4 jours) Oracle 8i/9i - Develop Web-based Applications with PL/SQL : 19 & 20.4.2004 (2 days) Introduction to ANSYS : 20 - 23.4.2004 (4 days) LabVIEW hands-on (E) : 20.4.2004 (afternoon, free of charge) FrontPage XP - niveau 2 : 26 & 27.4.2004 (2 jours) LabVIEW Base 2 : 6 & 7.5.2004 (2 jours) Word XP - niveau 1 : 10 & 11.5.2004 (2 jours) Word XP - niveau 2 : 24 & 25.5.2004 (2 jours) If you wish to participate in one of these courses, pleas...

  17. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Instructor-led WBTechT study or follow-up for Microsoft applications : 1.4.2004 (morning) FrontPage XP - niveau 1 : 5 & 6.4.2004 (2 jours) AutoCAD 2002 - niveau 1 : 19, 20.4 et 3, 4.5.2004 (4 jours) Oracle 8i/9i - Develop Web-based Applications with PL/SQL : 19 & 20.4.2004 (2 days) Introduction to ANSYS : 20 - 23.4.2004 (4 day...

  18. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: JAVA 2 Enterprise Edition - Part 1: WEB Applications: 21 & 22.2.2005 (2 days) FrontPage 2003 - niveau 1 : 21 & 22.2.2005 (2 jours) JAVA 2 Enterprise Edition - Part 2: Enterprise JavaBeans: 23 - 25.2.2005 (3 days) Utilisation des fichiers PDF avec ACROBAT 6.0 : 1.3.2005 (1 journée) ELEC-2005 - Spring Term: Integrated circuits and VLSI technology for physics: 1 - 17.3.2005 (6 X 2.30-hours lectures) C++ for Particle Physicists: 7 - 11.3.2005 (6 X 3-hour lectures) Joint PVSS JCOP Framework : 14 - 18.3.2005 (5 days) AutoCAD 2002 - niveau 1 : 15, 16, 21& 22.3.2005 (4 jours) FrontPage 2003 - niveau 2 : 21 & 22.3.2005 (2 jours) FileMaker - niveau 2 : 4 & 5.4.2005 (2 jours) EXCEL 2003 - niveau 2 : 11 & 12.4.2005 (2 jours) LabVIEW intermediate 1: 11 - 13....

  19. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: LabVIEW base 2/basics 2 : 3 & 4.2.2005 (2 jours/2 days - langue à décider/language to be decided) Finite State Machines in the JCOP Framework: 1 - 3.2.2005 (3 days - free course) The JAVA Programming Language Level 2: 7 - 9.2.2005 (3 days) JAVA 2 Enterprise Edition - Part 1: WEB Applications: 21 & 22.2.2005 (2 days) FrontPage 2003 - niveau 1 : 21 & 22.2.2005 (2 jours) JAVA 2 Enterprise Edition - Part 2: Enterprise JavaBeans: 23 - 25.2.2005 (3 days) ELEC-2005 - Spring Term: Integrated circuits and VLSI technology for physics: 1 - 17.3.2005 (6 X 2.30-hours lectures) C++ for Particle Physicists: 7 - 11.3.2005 (6 X 3-hour lectures) Joint PVSS JCOP Framework : 14 - 18.3.2005 (5 days) AutoCAD 2002 - niveau 1 : 15, 16, 21& 22.3.2005 (4 jours) FrontPage 20...

  20. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: ELEC-2005 - Spring Term: Integrated circuits and VLSI technology for physics: 1 - 17.3.2005 (6 x 2.5-hour lectures) C++ for Particle Physicists: 7 - 11.3.2005 (6 x 3-hour lectures) Joint PVSS JCOP Framework : 14 - 18.3.2005 (5 days) Oracle 9i: SQL: 14 -16.3.2005 (3 days) AXEL-2005; Introduction to Particle Accelerators : 14- 18.3.2005 (10 x 1 h lectures) ACCESS 2003 - Level 1: ECDL M5: 15 - 16.3.2005 (2 days) AutoCAD 2002 - niveau 1 : 15, 16, 21& 22.3.2005 (4 jours) FrontPage 2003 - niveau 2 : 21 & 22.3.2005 (2 jours) Hands-on Object-Oriented Design and Programming with C++ : 22 - 24.3.2005 (3 days) FileMaker - niveau 2 : 4 & 5.4.2005 (2 jours) Oracle 9i: Programming with PL/SQL: 4 - 6.4.2005 (3 days) EXCEL 2003 - niveau 2 : 11 & 12.4.2005 (2 jours) LabVIEW Intermedia...