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  1. Comparative study of SOS response induced by hydrogen peroxide in the absence or presence of iron ions, in Escherichia coli; Estudo comparativo da resposta SOS induzida pelo peroxido de hidrogenio em presenca e ausencia de ions ferro, em Escherichia coli

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Carlos Eduardo Bonacossa de

    1994-07-01

    The H{sub 2}O{sub 2} is an reactive oxygen specie that arises from cell respiration process. It may cause deleterious effects on cell, by reacting with transition metals like iron. In this way it yields free radicals that are able to damage organic molecules, mainly DNA. Recent works have suggested that in the absence of Fe ions H{sub 2}O{sub 2} still damages Escherichia coli DNA. This work presents a comparative analysis of cell SOS responses to DNA damage in Escherichia coli and Salmonella typhimurium mutants pretreated or not with a Fe{sup 2+} ion chelator (dipyridyl) and then treated with H{sub 2}O{sub 2}. The systems analysed were the lysogenic induction, Weigle reactivation, mutagenesis and cell inactivation curves. The cell inactivation curves were themselves distinct, in relation to both treatments. The increased sensitivity found in the lexA1 and recA13 mutants, when treated with dipyridyl and H{sub 2}O{sub 2}, suggests an important role of SOS response in repairing the lesions caused by this treatment. The profiles of the lysogenic induction and mutagenesis curves were also distinct in both treatments. The results of Weigle reactivation suggest that the products of uvrA and lexA genes have an important role in UV-damaged bacteriophage DNA repair, when dipyridyl-pretreated cells are treated with H{sub 2}O{sub 2}. All the results suggest that Fe-independent lesions produced by H{sub 2}O{sub 2} are different from the ones produced in the presence of this ion. (author)

  2. Asma induzida pelo exercício: aspectos atuais e recomendações

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    Laitano,Orlando; Meyer,Flávia

    2007-01-01

    OBJETIVO: Descrever os mecanismos da asma induzida pelo exercício (AIE), bem como os efeitos de diferentes tipos de treinamento físico na função pulmonar e nas capacidades aeróbia e anaeróbia. Destaca-se a importância de um diagnóstico correto mediante o teste de exercício e, no manejo, o uso de drogas beta-adrenérgicas e anticolinérgicas. FONTE DOS DADOS: Os artigos foram criteriosamente escolhidos utilizando as bases de dados PubMed e Scielo pelo ano de publicação e dando preferência a ensa...

  3. Associação entre perda auditiva induzida pelo ruído e zumbidos

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    Dias Adriano

    2006-01-01

    Full Text Available O estudo verificou a associação entre perda auditiva induzida por ruído (PAIR e queixa de zumbido em trabalhadores expostos ao ruído ocupacional. Foram entrevistados e avaliados trabalhadores com histórico de exposição ao ruído ocupacional atendidos em dois ambulatórios de audiologia. Estudou-se a existência de associação entre PAIR e ocorrência de zumbido por intermédio do ajuste de modelo de regressão logística, tendo como variável dependente o zumbido e como variável independente a PAIR, classificada em seis graus, controlada pelas co-variáveis idade e tempo de exposição ao ruído. Os dados foram coletados entre abril e outubro de 2003, na Cidade de Bauru, Estado de São Paulo, Brasil, contemplando 284 trabalhadores. Estimou-se que a prevalência de zumbido aumenta de acordo com a evolução do dano auditivo, controlado para a idade e tempo de exposição ao ruído. Os achados justificam o investimento em programas de conservação auditiva particularmente voltados para o controle da emissão de ruídos na fonte e para a intervenção na evolução das perdas auditivas geradas pela exposição ao ruído visando à manutenção da saúde auditiva e à diminuição dos sintomas associados.

  4. Repercussão hepática da carcinogênese colorretal induzida pelo azoximetano

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    Idália Maria Brasil Burlamaqui

    Full Text Available OBJETIVO: Avaliar as repercussões hepáticas da carcinogênese colônica induzida por diferentes doses e tempos de exposição ao azoximetano em ratos Wistar. MÉTODOS: Quarenta e quatro ratos foram distribuídos em quatro grupos. Os animais tinham oito semanas no início do experimento. No grupo 1, receberam 1.0mL de solução salina intraperitonealmente uma vez por semana por duas semanas. No grupo 2, receberam 15 mg/kg de azoximetano intraperitonealmente uma vez por semana por duas semanas. Esses animais foram mortos na 15ª semana do experimento. Os animais do grupo 3 receberam solução salina intraperitonealmente uma vez por semana por duas semanas. Os animais do grupo 4 receberam 20mg/kg de azoximetano intraperitonealmente uma vez por semana por duas semanas. Esses animais foram mortos na 26ª semana do experimento. Os fragmentos de tecido hepático foram corados pela hematoxilina e eosina e avaliadas microscopicamente. RESULTADOS: Grupo 1 e grupo 2 diferiram significantemente em relação a esteatose, mas não houve diferença entre o grupo 3 e o grupo 4. No entanto, no grupo 4 foram observadas lesões pré-neoplásicas (focos de células alteradas, claras, vacuoladas, basofílicas, anfofílicas, tigróides, oncocíticas, pequenas ou acidófilas, espongioses e pelioses e lesões neoplásicas (colangiomas e adenomas contendo hepatócitos atípicos de permeio, não identificados no grupo 3. CONCLUSÃO: No modelo de carcinogênese colorretal, lesões hepáticas pré-neoplásicas e neoplásicas aparecem e evoluem na proporção do tempo e dose de exposição ao azoximetano.

  5. Resposta cardiorrespiratória na asma induzida pelo exercício máximo com incrementos progressivos

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    LAMAR FILHO RUY AMAZONAS

    2001-01-01

    Full Text Available Objetivo: Verificar a ocorrência de broncoconstrição induzida por exercício e verificar a resposta cardiorrespiratória durante o exercício máximo em pacientes asmáticos. Pacientes e métodos: Quatorze asmáticos (VEF1 basal de 86,3%, conforme os critérios da American Thoracic Society, foram submetidos a teste de exercício máximo. Foram realizadas curvas fluxo-volume antes, sete e 15 minutos após esforço progressivo máximo. Seis indivíduos (43% apresentaram queda do VEF1 igual ou maior que 15% após esforço máximo (grupo I. Os demais constituíram o grupo II. No grupo I, observou-se redução no VEF1 de 40,9% e 26,7% sete e 15min após o exercício, enquanto no grupo II ocorreu diminuição de 2,6% e aumento de 1,2%. No pico do exercício, os dois grupos atingiram FCmax acima de 91% do valor de referência; a carga máxima foi de 82,7% e 62,5%, o O2max (mL/kg/min de 93,5% e 58,9% e a VEmax de 91,5% e 63,8%, respectivamente, nos grupos I e II. Essas diferenças foram estatisticamente significativas. A correlação da queda percentual do VEF1 (7min pós-exercício com a VEmax (%predito mostrou um coeficiente r = 0,8989 para o grupo I e um r = 0,3629 para o grupo II. Não se observou correlação estatisticamente significativa entre o delta VEF1 e o O2max (% predito. Conclusão: Nos pacientes com asma induzida pelo exercício, a ocorrência de broncoconstrição correlacionou-se com o nível de ventilação máxima, mas não com a aptidão física (O2 máximo.

  6. Levomepromazina e acepromazina no bloqueio da arritmia induzida pela adrenalina em cães anestesiados pelo halotano

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    Rezende Márlis Langenegger de

    2002-01-01

    Full Text Available Com este experimento, objetivou-se comparar os efeitos antiarritmogênicos da levomepromazina e da acepromazina em cães anestesiados pelo halotano e submetidos a doses crescentes de adrenalina. Foram empregados 19 animais adultos, sadios, separados em dois grupos, sendo um de 10 (G1 e outro de 09 (G2. O G1 recebeu, por via intravenosa, levomepromazina, na dose de 1mg/kg, seguida 15 minutos após, pela aplicação de propofol, pela mesma via, na dose de 5 ± 1,3mg/kg. Procedeu-se à intubação orotraqueal e iniciou-se a administração de halotano, diluído em oxigênio, na concentração de 3 CAM (2,5 V%, em circuito anestésico semi-fechado. Decorridos 50 minutos da indução anestésica, iniciou-se a administração contínua, intravenosa, de solução de adrenalina a 4% em doses crescentes de 4, 5, 6, 7 e 8mig/kg/min, com incremento da dose a intervalos de 10 minutos. Para o G2, empregou-se a mesma metodologia, substituindo-se a levomepromazina pela acepromazina, na dose de 0,1mg/kg. A eletrocardiografia e as pressões arteriais (sistólica, diastólica e média dos animais permaneceram sob monitoramento contínuo, com início imediatamente antes da aplicação dos fármacos e término ao final do período experimental. As colheitas dos valores numéricos tiveram início imediatamente antes da aplicação dos fármacos (M1, seguidas de novas mensurações realizadas nos momentos correspondentes às doses crescentes de adrenalina (4, 5, 6, 7, 8mig/kg/minuto; M2, M3, M4, M5, M6, respectivamente. Os valores obtidos na eletrocardiografia referem o número total de batimentos ventriculares ectópicos correspondentes às doses crescentes de adrenalina. Apenas um animal de cada grupo apresentou arritmia ventricular sustentada, sendo que o animal pré-tratado com acepromazina foi à óbito. Os resultados obtidos permitiram concluir que a levomepromazina e a acepromazina minimizam a arritmia ventricular induzida pela adrenalina, nas doses empregadas

  7. Alterações fetais induzidas pelo uso de antiinflamatórios durante a gestação

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    Fonseca Cláudia Sampaio

    2002-01-01

    Full Text Available Em geral, todos os efeitos dos antiinflamatórios estão relacionados com a inibição da ciclo-oxigenase (COX do ácido araquidônico e, portanto, inibição da produção de prostaglandinas e tromboxanos. Existem dois tipos de COX, quais sejam COX-1 e COX-2. A COX-1 é uma enzima constitucional expressa em muitos tecidos, incluindo plaquetas sangüíneas, e está envolvida na homeostase tecidual. Por outro lado, a COX-2 é induzida em células inflamatórias quando elas são ativadas, sendo considerada a enzima que produz os mediadores da inflamação da classe dos prostanóides. A ação dos antiinflamatórios está relacionada à inibição da COX-2 e é provável que seus efeitos indesejados se devam principalmente à inibição da COX-1. Tratamentos maternos com antiinflamatórios não esteroidais (AINEs têm sido associados, com freqüência, à vasoconstrição do ducto arterioso fetal, hipertensão arterial pulmonar e inibição da agregação plaquetária. Alterações na hemostasia são alguns dos efeitos colaterais produzidos pelo uso incontrolado dos AINEs, os quais induzem a um desequilíbrio na liberação de prostaglandinas e tromboxanos, que se reflete na adesividade e agregação plaquetária. As alterações hemostáticas observadas em neonatos, decorrentes do uso de salicilatos pela mãe, ocorrem devido à inibição da agregação plaquetária e à diminuição da atividade do fator XII relacionado à coagulação sangüínea. Estudos em camundongos revelaram que o uso de corticóides durante a gestação pode levar a anormalidades no desenvolvimento fetal, por alterações na diferenciação celular.

  8. Perda auditiva induzida pelo ruído em trabalhadores de metalúrgica Noise induced hearing loss in metallurgical workers

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    Simone Adad Araújo

    Full Text Available Introdução: A perda auditiva induzida pelo ruído é uma patologia insidiosa cumulativa, que cresce ao longo dos anos de exposição ao ruído associado ao ambiente de trabalho. Objetivos: Identificar e quantificar a ocorrência de alterações auditivas sugestivas de Perda Auditiva Induzida pelo Ruído e os principais sintomas otorrinolaringológicos referidos pelos trabalhadores. Forma de estudo: prospectivo clínico randomizado. Casuística e métodos: Pesquisa realizada no período de janeiro a março de 2000 com 187 trabalhadores de indústria metalúrgica no município de Goiânia, avaliados por médicos otorrinolaringologistas através de roteiro de entrevista e audiometria ocupacional. Resultados: Foram obtidas audiometrias ocupacionais sendo: 21% sugestivas de PAIR, 72%, normais e 7%, sugestivas de outras doenças auditivas. Os sintomas auditivos mais freqüentes foram: dificuldade de compreensão da fala, 12%; hipoacusia, 7%; tinitus, 13%; sensação de plenitude auricular, 4%; otorreia, 6%; tonturas, 12%. Conclusão: Concluímos que em metalúrgica há ocorrência de alterações auditivas sugestivas de Perda Auditiva Induzida pelo Ruído e queixa de sintomas otorrinolaringológicos significativos.Introduction: Noise induced hearing loss is a cumulative pathological disease that increases over the years with exposure to noise associated with the work atmosphere. Objectives: To identify and quantify the occurrence of hearing loss suggestive of Noise Induced Hearing Loss (NIHL and the principle otolaryngological symptoms referred to by workers. Study design: prospective clinical randomized. Material and method: Research was carried out from January to March of 2000 with 187 metallurgical workers in the city of Goiânia, who were evaluated by otolaryngologists by means of a combination of interviews and occupational hearing exams. Results: Occupational hearing tests results were: 21% suggestive of NIHL, 72% normal, and 7% suggestive of

  9. Associação da perda auditiva induzida pelo ruído com o tempo acumulado de trabalho entre motoristas e cobradores

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    Ricardo Cordeiro

    Full Text Available Através da técnica da regressão linear múltipla, estudou-se a relação existente entre a perda auditiva induzida pelo ruído e as variáveis "tempo total acumulado de trabalho como condutor de veículos coletivos urbanos", "pressão arterial diastólica" e "idade", em uma população de 278 motoristas e cobradores usuários de um serviço de saúde ocupacional da cidade de Campinas, estado de São Paulo (Brasil. Os principais resultados encontrados foram uma associação positiva entre a perda auditiva e o tempo acumulado de trabalho, bem como a existência de uma interação entre esta variável e a idade dos condutores.

  10. Antioxidantes da dieta como inibidores da nefrotoxicidade induzida pelo antitumoral cisplatina Dietary antioxidants as inhibitors of cisplatin-induced nephrotoxicity

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    Lusânia Maria Greggi Antunes

    2004-03-01

    Full Text Available A cisplatina é uma droga antineoplásica altamente efetiva contra vários tipos de cânceres humanos, tais como tumores do testículo e ovário, câncer da cabeça e pescoço e câncer do pulmão. Entretanto, a nefrotoxicidade é um dos principais efeitos colaterais da terapia com a cisplatina. A gravidade da nefrotoxicidade induzida pela cisplatina está relacionada com a concentração de platina nos rins. As evidências mostram que a nefrotoxicidade induzida pela cisplatina é atribuída ao dano oxidativo resultante da geração de radicais livres, e que a administração de antioxidantes é eficiente na inibição destes efeitos colaterais. Uma abordagem alternativa para proteger os roedores dos efeitos colaterais da cisplatina é o uso de conhecidos antioxidantes da dieta. Alguns estudos têm sido realizados para diminuir a peroxidação lipídica e os efeitos citotóxicos induzidos pela cisplatina, com o emprego de antioxidantes da dieta, tais como, selenito de sódio, vitaminas C e E, curcumina e o carotenóide bixina. Nós sugerimos que aqueles antioxidantes da dieta têm efeito nefroprotetor, e que os mecanismos antioxidantes destes compostos deveriam ser explorados durante a quimioterapia com a cisplatina.Cisplatin is a highly effective antineoplastic drug used against several types of human cancers, such as testicular and ovarian tumors; head and neck; and lung cancer. However, nephrotoxicity is one of the most important side-effects of cisplatin therapy. The severity of cisplatin nephrotoxicity is related to platinum concentration in the kidneys. There is a growing amount of evidence that cisplatin-induced nephrotoxicity is ascribed to oxidative damage resulting from free radical generation and that the administration of antioxidants is efficient in inhibiting these side effects. An alternative approach aiming to protect rodents against cisplatin side-effects is the introduction of known dietary antioxidants. Some studies have been

  11. Caracterização funcional da hipertrofia miocárdica induzida pelo isoproterenol e de sua regressão

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    Neif Murad

    2001-07-01

    Full Text Available OBJETIVO: Analisar as disfunções da hipertrofia miocárdica induzida pelo isoproterenol e de sua regressão. Corações isolados hipertrofiados por isoproterenol (ISO (8 dias e após 22 dias de sua suspensão (regressão foram distendidos. MÉTODOS: Até pressão de repouso (Pr de 60mmHg, analisaram-se: pressão desenvolvida máxima (PDmáx.; estresse sistólico (sigmamáx; inclinação da reta estresses/deformações; constante de relaxamento; rigidez da câmara e rigidez miocárdica. RESULTADOS: Nos corações hipertrofiados (H as variações de volume (deltaV necessárias para Pr=60mmHg foram heterogêneas. Em alguns (H1; n=10 deltaV equivaleu à dos controle (C enquanto em outros (H2; n=10 foi inferior, e também diferiram quanto ao peso seco, complacência ventricular, rigidez miocárdica, constante de relaxamento,e sigmamáx. PDmáx dos grupos H1 e H2 foram superiores às de C (n=8 e Regressão (R (n=8. Contudo, sigmamáx de H2 foi menor que C, H1 e R. O mecanismo de Frank-Starling foi deprimido nos corações hipertrofiados. A constante de relaxamento de H2 indicou retardo no decaimento da pressão associado a menor complacência ventricular e rigidez miocárdica acentuada. CONCLUSÃO: Hipertrofia miocárdica induzida pelo ISO não é homogênea. Alguns corações têm alterações pouco expressivas; outros têm comprometimento das funções sistólica e diastólica. A hipertrofia miocárdica reduz a capacidade de gerar força e aprimora a capacidade em variar pressão por aumento da relação massa/volume. Há, também, comprometimento da complacência ventricular e da rigidez muscular.

  12. Tratamento farmacológico da hiperalgesia experimentalmente induzida pelo núcleo pulposo Pharmacologic treatment of hyperalgesia experimentally induced by nucleus pulposus

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    André Luiz de Souza Grava

    2010-01-01

    Full Text Available OBJETIVO: Avaliar o efeito de drogas anti-inflamatórias (dexametasona, indometacina, atenolol, indometacina e atenolol e analgésica (morfina sobre a hiperalgesia experimentalmente induzida pelo núcleo pulposo em contato com o gânglio da raiz dorsal de L5. MÉTODOS: Trinta ratos Wistar machos com peso de 220 a 250g foram utilizados no estudo. A indução da hiperalgesia foi realizada por meio do contato de fragmento de núcleo pulposo retirado da região sacrococcígea e colocado sobre o gânglio da raiz dorsal de L5. Os 30 animais foram divididos em grupos experimentais de acordo com a droga utilizada. As drogas foram administradas durante duas semanas a partir da realização do procedimento cirúrgico para a indução da hiperalgesia. A hiperalgesia mecânica e térmica foram avaliadas por meio do teste da pressão constante da pata, von Frey eletrônico e Hargraves por um período de sete semanas. RESULTADOS: A maior redução da hiperalgesia foi observada no grupo de animais tratados pela morfina, seguido pela dexametasona, indometacina e atenolol. A redução da hiperalgesia foi observada após a interrupção da administração das drogas, com exceção do grupo de animais tratados com morfina, nos quais ocorreu aumento da hiperalgesia após a interrupção do tratamento. CONCLUSÕES: A hiperalgesia induzida pelo contato do núcleo pulposo com o gânglio da raiz dorsal pode ser reduzida com a administração de anti-inflamatórios e analgésicos, tendo sido observado a maior redução da hiperalgesia com a administração da morfina e dexametasona.OBJECTIVE: To evaluate the effect of antiinflammatory (dexamethasone, indomethacin, atenolol, indomethacin and atenolol and analgesic drugs (morphine on hyperalgesia experimentally induced by nucleus pulposus (NP contact with the L5- dorsal root ganglion (DRG. METHODS: Thirty male Wistar rats with weights ranging from 220 to 250 g were used in the study. The hyperalgesia was induced by

  13. Quebra do grão em Resíduos de Construção Civil (RCC induzida pelo processo de compactação

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    Nathália Marques da Silva

    Full Text Available Resumo Nas últimas décadas a geração de resíduos de construção civil (RCC tornou-se um fator relevante em todo o mundo. Dessa forma, é cada vez mais importante o reaproveitamento desse material, sobretudo em obras de grande consumo, tais como as obras rodoviárias. No entanto, a aplicação desses resíduos depende do conhecimento das propriedades químicas e mecânicas do material. Entre esses parâmetros destaca-se que a susceptibilidade à quebra do grão é de fundamental importância. Isso porque esse parâmetro influencia diretamente no comportamento do material através da resistência e da permeabilidade. O presente trabalho avaliou a quebra do grão em RCC induzida pelo processo de compactação através da aplicação das energias normal e modificada. Para isso o material foi dividido em frações com granulometrias diferentes. Dessa forma, foi analisada a influência da energia, do tamanho do grão, da mineralogia, do teor de umidade e da densidade da amostra. Os resultados indicaram que a ocorrência da quebra do grão está diretamente relacionada com a constituição mineralógica, observando-se que a maior fração estudada foi aquela que sofreu a menor quebra dos grãos. Adicionalmente, os resultados indicaram que a densidade de compactação da amostra também possui significativa influência no processo de quebra dos grãos.

  14. Características nociceptivas e histomorfométricas de nervos medianos de ratos com obesidade induzida pelo glutamato monossódico = Nociceptive and histomorphometric characteristics of median nerves of rats with obesity induced by monosodium glutamate

    OpenAIRE

    Coradini, Josinéia Gresele

    2014-01-01

    Objetivos: Comparar a nocicepção e, histomorfometricamente, a secção transversa de nervos periféricos de ratos Wistar submetidos ao modelo de obesidade induzida pelo glutamato monossódico com animais controle Métodos: Foram utilizados ratos Wistar, divididos em grupo obeso e grupo controle. Desde o nascimento, durante os primeiros cinco dias de vida, os ratos do grupo obeso receberam uma injeção diária subcutânea de glutamato monossódico (4g/kg de peso corporal/dia), enquanto os ratos do g...

  15. Ocorrência de perda auditiva induzida pelo ruído em carpinteiros Occurrence of noise induced hearing loss in carpenters

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    Victor Hygor Veríssimo Farias

    2012-06-01

    Full Text Available OBJETIVO: investigar a ocorrência de perda auditiva induzida pelo ruído (PAIR em carpinteiros, caracterizando a perda auditiva por faixa etária, tempo de exposição total ao ruído e uso regular de protetores auditivos durante o tempo total de exposição. MÉTODO: estudo retrospectivo, descritivo, em uma população de 80 carpinteiros da construção civil, atendidos em uma clínica particular. Foram analisados 60 trabalhadores, conforme dados obtidos na anamnese e ficha do exame audiométrico. RESULTADOS: 49% dos trabalhadores apresentaram audição normal, sendo 58% com limiares auditivos normais bilateralmente e 35% com entalhe audiométrico em 3 kHz, 4 kHz e/ou 6 kHz. 44% apresentaram perfil audiométrico sugestivo de PAIR, destes 74% foram classificados como PAIR bilateral e 19% como PAIR unilateral. Houve diferença estatística significante entre os grupo PAIR e Normal em relação às variáveis idade (p=0,001, assim como o tempo total de exposição ao ruído ocupacional (p=0,002. CONCLUSÃO: quanto maior a idade e o tempo de profissão como carpinteiro, maior é a sua alteração auditiva, principalmente, devido à exposição ao ruído elevado durante a jornada de trabalho, sendo também constatado que as medidas de controle pelo uso do protetor são insuficientes para prevenir perdas auditivas. Portanto, sugerem-se medidas preventivas em saúde auditiva ativamente nessa população estudada, no ramo da construção civil.PURPOSE: to investigate the occurrence of noise induced hearing loss in carpenters, characterizing the hearing loss for age group, time of total exposure to noise and regular use of hearing protectors during the total exposure time. METHOD: retrospective and descriptive study in a population of 80 construction carpenters, attended at a private clinic. 60 workers were analyzed, as data on medical history and record of audiometric testing. RESULTS: 49% of the workers shoed normal hearing, being 58% with normal

  16. Enalaprilato na prevenção da hipertrofia ventricular esquerda induzida pelo isoproterenol Enalaprilat prevents the left ventricular hypertrophy induced by isoproterenol

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    Eduardo A. S. Costa

    1997-07-01

    Full Text Available OBJETIVO: Avaliar se o enalaprilato, droga inibidora da enzima de conversão da angiotensina I, previne a hipertrofia ventricular esquerda (HVE induzida pelo isoproterenol. MÉTODOS: Foram divididos em 4 grupos, 72 ratos Wistar-EPM: CON controle; ENA, tratados com enalaprilato (1mg/kg via subcutânea (sc por 8 dias; ISO, tratados com isoproterenol (0,3mg/kg via sc/8 dias e ENA+ISO, tratados simultaneamente com ambas as drogas. Em 10 animais de cada grupo foram determinadas a freqüência cardíaca (FC e a pressão arterial (PA e verificado o peso de ventrículo esquerdo (VE. Em 8 animais de cada grupo, fragmento do VE foi corado com hematoxilina-eosina e picro-sírius e preparado para estudo morfométrico e ultra-estrutural, respectivamente, com microscópio de luz e eletrônico. RESULTADOS: Nos grupos estudados (CON, ENA, ISO e ISO+ENA não ocorreram variações na PA. Os grupos ISO e ISO+ENA exibiram aumentos significantes na FC. O grupo ISO apresentou aumento significativo do peso do VE (PU= 0,821g e PS= 0,204g, quando comparado ao grupo CON. O grupo ENA não exibiu modificação de peso do VE quando comparado ao grupo CON (PU= 0,590g e PS= 0,139g. No grupo ENA+ISO (PU= 0,737g e PS= 0,177g constatou-se diferença de peso ao ser comparado aos grupos ISO e CON. A análise morfométrica e ultra-estrutural mostraram que o ISO induziu hipertrofia dos cardiomiócitos e aumento do tecido conjuntivo com depósito de fibras colágenas do tipo I. O enalaprilato associado com isoproterenol atenuou importantemente aquela manifestação. CONCLUSÃO: O enalaprilato inibiu a ação do isoproterenol sobre os cardiomiócitos, evitando parcialmente, na dose utilizada, a HVE e diminuindo também a quantidade de fibras colágenas.PURPOSE: To evaluate whether the enalaprilat, angiotensin I enzyme conversion inhibitor, could prevent the left ventricular hypertrophy (LVH induced by isoproterenol. METHODS: Seventy two adult Wistar-EPM rats were divided into four

  17. Doença pulmonar induzida pelas radiações ou pelos fármacos citostáticos

    Directory of Open Access Journals (Sweden)

    Ulrich Costabel

    2000-03-01

    Full Text Available RESUMO: O artigo discute dois temas afins: 1 doença pulmonar induzida pela radioterapia nas suas diferentes apresentações: pneumonite de radiação clássica e fibrose pulmonar e outras duas mais recentemente descritas, BOOP e alveolite linfocitária bilateral São discutidos os mecanismos patoeenicos, clinic:a e tratamento; 2 a patologia intersticial induzida por fármacos citostáticos nas suas várias formas de apresentação.REV PORT PNEUMOL 2000; VI (2: 141-144 ABSTRACT: In this paper two topics art adressed: 1 the radiation induced lung injury in its possible forms: classical radiation pneumonitis and radiation fibrosis or two other newly recoenised sindromes BOOP and bilateral lymphodtic alveolitis. The mecanism and treatment are discussed; 2 the cytotocic drug-induced lung diseases and their pulmonary reaction patterns.REV PORT PNEUMOL 2000; VI (2: 141-144 Palavras-chave: Radioterapia, Citostaticos, BOOP, Alveolite Linfocitica, Key-words: Radiation, Cytotocic Drugs, Lymphocitic Alveolitis

  18. Prevalência de coilocitose em biópsias penianas de parceiros de mulheres com lesões genitais induzidas pelo HPV Prevalence of koilocytosis in penile biopsies of partners of women with HPV-induced genital lesions

    Directory of Open Access Journals (Sweden)

    Alberto Azoubel Antunes

    2004-01-01

    Full Text Available OBJETIVO: determinar a prevalência de coilocitose em biópsias penianas de parceiros de mulheres com lesões genitais induzidas pelo HPV e analisar os fatores de risco envolvidos com a infecção peniana. MÉTODOS: foram incluídos neste estudo de coorte transversal 80 parceiros de mulheres com lesões induzidas pelo HPV no período de maio de 2002 a junho de 2003. A média de idade dos pacientes foi de 34,1 anos (variando de 16 a 63. Todos os pacientes negavam ou desconheciam qualquer tipo de lesão genital. Foram submetidos à anamnese específica quanto a presença de sintomas uretrais, passado de doenças sexualmente transmissíveis (DST, número de parceiras sexuais, uso de preservativos e postectomia. Posteriormente foram submetidos à peniscopia com a utilização de soluções de ácido acético e azul de toluidina, tendo sido colhido material para biópsia das lesões de aspecto morfológico sugestivo de contaminação por HPV. A análise estatística foi realizada pelo teste do chi2. RESULTADOS: o exame foi considerado negativo em 24 pacientes (30%. Todos os outros 56 pacientes (70% apresentaram lesões acetobrancas e azul de toluidina positivas. Destes, 53 foram biopsiados, e 41 pacientes (77,3% apresentaram biópsia com alterações histológicas sugestivas de infecção pelo HPV. CONCLUSÕES: a prevalência da infecção genital masculina pelo HPV foi de 51,2%, e nenhum fator de risco analisado foi associado com uma maior prevalência da infecção genital pelo vírus.PURPOSE: to determine the prevalence of koilocytosis in penile biopsies of partners of women with HPV-induced genital lesions, and to analyze the risk factors associated with the penile viral infection. METHODS: a total of 80 partners of HPV-infected women with genital lesions were included in this transversal cohort study. The study was carried out between May 2002 and June 2003. The mean age was 34.1 years (16 to 63. No patient reported any kind of genital lesion

  19. Avaliação do uso do captopril na fibrose peritoneal induzida em ratos pelo uso de solução de glicose a 4,25%

    Directory of Open Access Journals (Sweden)

    Adriana Fátima Menegat Schuinski

    2013-12-01

    Full Text Available INTRODUÇÃO: A Insuficiência Renal Crônica (IRC tem incidência alarmante neste século. A diálise peritoneal, uma das modalidades de terapia renal pode ter complicações, e entre estas a fibrose peritoneal, que ocorre com o decorrer dos anos nestes pacientes. Sua forma mais grave é a chamada peritonite esclerosante encapsulante, levando à mudança de terapia dialítica. OBJETIVO: Estudar a influência do uso do captopril na fibrose peritoneal induzida em ratos pelo uso de solução de glicose a 4,25 %. MÉTODOS: Estudo prospectivo controlado, em ratos Wistar não urêmicos. Foram estudados 20 animais. Os animais foram submetidos diariamente à punção abdominal, sendo infundida solução de diálise peritoneal com glicose a 4,25% na dose de 10 ml/100 g de peso. Os animais foram divididos em 2 grupos: experimental e controle. O grupo experimental recebeu captopril na dose de 30 mg/kg/dia por gavagem. O grupo controle não recebeu nenhuma droga. Foram acompanhados por 21 e 49 dias. Ao final do período foram submetidos à procedimento cirúrgico para retirada de peritônio parietal e visceral. As amostras obtidas foram analisadas histologicamente, usando-se coloração Hematoxilina - Eosina e Sirius Red, para avaliação do grau de fibrose. RESULTADOS: A análise mostrou que a intensidade da fibrose, a espessura do peritônio e o número de células não atingiram diferença estatisticamente significante entre os grupos experimental e controle. CONCLUSÃO: O estudo mostrou que o uso do captopril não foi capaz de alterar a intensidade da fibrose peritoneal induzida pelo uso de solução de diálise em ratos.

  20. Alterações neuroquímicas e morfológicas induzidas pelo hipertireoidismo no hipocampo de ratos imaturos

    OpenAIRE

    Andrade, Camila Mariana

    2014-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas, Programa de Pós-Graduação em Bioquímica, Florianópolis, 2014. O hipertireoidismo está relacionado a diversas manifestações neurológicas. Todavia, os mecanismos precisos pelos quais os HT induzem seus efeitos no cérebro durante o desenvolvimento ainda são controversos. Os objetivos deste trabalho foram analisar o efeito desta patologia sobre a indução de estresse oxidativo, bem como as defesas an...

  1. Associação entre perda auditiva induzida pelo ruído e zumbidos Association between noise-induced hearing loss and tinnitus

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    Adriano Dias

    2006-01-01

    Full Text Available O estudo verificou a associação entre perda auditiva induzida por ruído (PAIR e queixa de zumbido em trabalhadores expostos ao ruído ocupacional. Foram entrevistados e avaliados trabalhadores com histórico de exposição ao ruído ocupacional atendidos em dois ambulatórios de audiologia. Estudou-se a existência de associação entre PAIR e ocorrência de zumbido por intermédio do ajuste de modelo de regressão logística, tendo como variável dependente o zumbido e como variável independente a PAIR, classificada em seis graus, controlada pelas co-variáveis idade e tempo de exposição ao ruído. Os dados foram coletados entre abril e outubro de 2003, na Cidade de Bauru, Estado de São Paulo, Brasil, contemplando 284 trabalhadores. Estimou-se que a prevalência de zumbido aumenta de acordo com a evolução do dano auditivo, controlado para a idade e tempo de exposição ao ruído. Os achados justificam o investimento em programas de conservação auditiva particularmente voltados para o controle da emissão de ruídos na fonte e para a intervenção na evolução das perdas auditivas geradas pela exposição ao ruído visando à manutenção da saúde auditiva e à diminuição dos sintomas associados.The objective of this study was to verify an association between noise-induced hearing loss (NIHL and tinnitus in workers exposed to occupational noise. Workers with a history of work noise exposure who attended two audiology outpatient clinics were interviewed and evaluated. The statistical association between NIHL and tinnitus was evaluated using an adjusted linear regression model, with tinnitus as the dependent variable and NIHL, classified into six levels, as the independent variable, with age and length of noise exposure as covariates. Data were collected from 284 workers between April and October, 2003, in Bauru, São Paulo State, Brazil. Tinnitus was observed to increase with the progression of auditory damage, controlled by age

  2. Exposição repetida à cafeína aumenta a atividade locomotora induzida pelo femproporex em ratos adolescentes e adultos Repeated administration of caffeine increases femproporex-induced locomotor activity in adolescent and adult rats

    Directory of Open Access Journals (Sweden)

    Ana Helena Paro

    2008-09-01

    Full Text Available A cafeína e o femproporex são substâncias psicoestimulantes. O femproporex é muito utilizado no Brasil como anorexígeno enquanto a cafeína é amplamente consumida como constituinte regular da dieta. A administração repetida de psicoestimulantes induz sensibilização comportamental que se caracteriza pelo aumento progressivo dos seus efeitos locomotores. Pode ocorrer ainda sensibilização cruzada entre essas substâncias. Investigamos se a administração repetida de cafeína aumenta a locomoção induzida pelo femproporex em ratos adolescentes e adultos. Quarenta e oito ratos adolescentes (dia pós-natal 27 e 32 adultos (dia pós-natal 60 foram distribuídos em dois grupos que receberam injeção intra-peritoneal de 10,0 mg/kg de cafeína (CAF (adolescentes N = 24; adultos N = 16 ou salina (SAL (adolescentes N = 24; adultos N = 16 diariamente durante 10 dias. Três dias após a última injeção, cada grupo CAF ou SAL foi subdividido em dois subgrupos que receberam injeção i.p. de salina (SAL (1 mL/kg ou femproporex (FEM (2,0 mg/kg. Após as injeções, a atividade locomotora foi avaliada automaticamente em intervalos de 5 minutos durante 1 hora. Nossos resultados demonstraram que em ratos adolescentes e adultos o pré-tratamento com CAF aumenta a atividade locomotora induzida pela administração aguda de FEM, sugerindo que a cafeína causa sensibilização aos efeitos locomotores desse derivado anfetamínico.Caffeine and femproporex are psychostimulants drugs widely consumed in Brazil. Behavioral sensitization is defined as an augmentation in the behavioral effect of a psychostimulant upon re-administration. Repeated administration of a psychostimulant produces behavioral sensitization to that drug and cross-sensitization to other drugs. We investigated whether repeated administration of caffeine increases femproporex-induced locomotor activity in adolescent and adult rats. Forty-eight adolescent (postnatal day 27 and 32 adult

  3. Does troncular vagotomy modify the proliferative gastric lesions induced in rats by duodenogastric reflux? A vagotomia troncular modifica as lesões proliferativas gástricas induzidas em ratos pelo refluxo duodenogástrico?

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    Paulo Antônio Rodrigues

    2007-06-01

    Full Text Available PURPOSE: to investigate if combining VT to DGR through the pylorus can modulate the biological behavior of PL induced by DGR and to verify if TV alone can induce morphologic lesions in the gastric mucosa. METHODS: 62 male Wistar rats were assigned to four groups: 1 - Control (CT gastrotomy; 2 - Troncular Vagotomy (TV plus gastrotomy; 3 - Duodenogastric reflux through the pylorus (R and 4 - Troncular vagotomy plus DGR (RTV. The animals were killed at the 54 week of the experiment. DGR was obtained by anastomosing a proximal jejunal loop to the anterior gastric wall. TV was performed through isolation and division of the vagal trunks. Gastrotomy consisted of 1 cm incision at the anterior gastric wall. PL were analyzed gross and histologically in the antral mucosa, at the gastrojejunal stoma and at the squamous portion of the gastric mucosa. RESULTS: Groups R and RTV developed exophytic lesions in the antral mucosa (R=90.9%; RTV=100% and at the gastrojejunal stoma (R=54.54%; RTV=63.63%. Histologically they consisted of proliferative benign lesions, without cellular atypias, diagnosed as adenomatous hyperplasia. Both groups exposed to DGR presented squamous hyperplasia at the squamous portion of the gastric mucosa (R= 54.5%; RTV= 45.4%. TV, alone, did not induce gross or histological alterations in the gastric mucosa. TV did note change the morphologic pattern of the proliferative lesions induced by DGR. CONCLUSIONS: DGR induces the development of PL in the pyloric mucosa and at the gastrojejunal stoma. TV does not change the morphologic pattern of the proliferative lesions induced by DGR. TV alone is not able to induce morphologic lesions in the gastric mucosa.OBJETIVO: investigar se a adição da VT ao RDG através do piloro, interfere no comportamento biológico das LP induzidas pelo RDG e observar se a VT isoladamente leva ao desenvolvimento de lesões morfológicas na mucosa gástrica. MÉTODOS: Foram utilizados 62 ratos Wistar machos, distribu

  4. Doença de depósito lisossomal induzida pelo consumo de Ipomoea verbascoidea (Convolvulaceae em caprinos no semiárido de Pernambuco

    Directory of Open Access Journals (Sweden)

    Dayane D.C.C. Lima

    2013-07-01

    s dos caprinos do grupo controle, e os neurônios de Purkinje estavam atróficos. Sugere-se que essas alterações sejam responsáveis pelo quadro clínico neurológico observado nos caprinos que deixam de ingerir a planta e apresentam seqüelas da intoxicação.

  5. Elevação dos níveis séricos de laminina na cirrose hepática induzida pelo tetracloreto de carbono

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    Neves Lindalva Batista

    2003-01-01

    Full Text Available RACIONAL: Laminina sérica tem sido correlacionada com a hipertensão portal e a capilarização dos sinusóides na doença hepática crônica. Pouco é conhecido sobre sua dinâmica nas doenças hepáticas. OBJETIVO: Estudar os níveis séricos de laminina e correlacioná-los com graus de fibrose hepática e hipertensão portal em ratos tratados com tetracloreto de carbono. MATERIAL E MÉTODO: Quarenta e nove ratos albinos Wistar foram mantidos como controles (n = 16 ou tratados com solução de tetracloreto de carbono a 8% (n = 23. Entre a 6ª e 16ª semana de experimento animais foram sacrificados após cateterização da veia porta e medida da pressão portal. Fragmentos de fígado foram fixados em formol para microscopia óptica. A fibrose hepática foi classificada em perivenular, septal incompleta e completa e cirrose. Concentração de laminina circulante foi determinada pela técnica de ensaio imunoenzimático com anticorpo desenvolvido a partir de laminina isolada de tumor Engelbreth-Holm-Swarm e purificado em coluna de afinidade. RESULTADOS: A pressão portal correlacionou-se com grau de fibrose hepática (r = 0,82; n = 45 e seus níveis nos grupos fibrose septal (10,8 ± 1,2 cm H2O e cirrose (13,6 + 3,1 cm H2O foram estatisticamente superiores aos observados no grupo-controle (7,9 + 1,5 cmH2O e no grupo com fibrose perivenular (9,1 + 0,8 cmH2O. A concentração de laminina no sangue periférico dos cirróticos (40,0 + 18,7 µg/dL foi significativamente superior aos grupos-controle (13,8 + 12,1 µg/dL, fibrose perivenular (19,1 + 15,5 e fibrose septal (22,2 + 27,0 µg/dL. Laminina circulante correlacionou-se de maneira significativa com o grau de fibrose hepática (r = 0,59; n = 49 e com os níveis de pressão portal (r = 0,29; n = 45. CONCLUSÕES: Na intoxicação crônica experimental pelo tetracloreto de carbono, níveis circulantes de laminina correlacionam-se mais com o grau de fibrose hepática do que com os níveis de hipertens

  6. Asma induzida pelo exercício: aspectos atuais e recomendações Asma inducido por el ejercicio: aspectos actuales y recomendaciones Exercise-induced asthma: current aspects and recommendations

    Directory of Open Access Journals (Sweden)

    Orlando Laitano

    2007-02-01

    Full Text Available OBJETIVO: Descrever os mecanismos da asma induzida pelo exercício (AIE, bem como os efeitos de diferentes tipos de treinamento físico na função pulmonar e nas capacidades aeróbia e anaeróbia. Destaca-se a importância de um diagnóstico correto mediante o teste de exercício e, no manejo, o uso de drogas beta-adrenérgicas e anticolinérgicas. FONTE DOS DADOS: Os artigos foram criteriosamente escolhidos utilizando as bases de dados PubMed e Scielo pelo ano de publicação e dando preferência a ensaios clínicos randomizados, com critérios de seleção da amostra bem definidos. SÍNTESE DOS DADOS: Os mecanismos para explicar a AIE permanecem sem conclusão, mas parece haver uma interação fisiológica entre as hipóteses aqui apresentadas. O uso de medicamentos e as freqüentes crises durante o exercício aparecem como fatores limitantes para a prática de exercícios físicos, conduzindo para um estilo de vida sedentário. CONCLUSÃO: Deve-se incentivar a prática de exercícios devidamente prescritos e minimizar as restrições aos sujeitos com AIE.OBJETIVO: Describir los mecanismos del asma inducido por el ejercicio (AIE, así como los efectos de diferentes tipos de entrenamiento físico sobre la función pulmonar y las capacidades aeróbica y anaeróbica. Se destaca la importancia de un diagnóstico correcto mediante el test de ejercicio y el manejo o uso de drogas beta-adrenérgicas y anticolinérgicas. FUENTES: Los artículos fueron cuidadosamente escogidos utilizando las bases de dados de PubMed y Scielo por el año de publicación, dando preferencia a ensayos clínicos randomizados, con criterios de selección de muestra bien definidos. SÍNTESES DE DATOS: Los mecanismos para explicar la AIE permanecen sin conclusión, pero parece haber una interacción fisiológica entre las hipótesis aquí presentadas. El uso de medicamentos y las frecuentes crisis durante el ejercicio aparecen como factores limitantes para la práctica de

  7. Nominal SOS

    NARCIS (Netherlands)

    Cimini, M.; Mousavi, M.R.; Reniers, M.A.; Gabbay, M.J.

    2012-01-01

    Plotkin's style of Structural Operational Semantics (SOS) has become a de facto standard in giving operational semantics to formalisms and process calculi. In many such formalisms and calculi, the concepts of names, variables and binders are essential ingredients. In this paper, we propose a formal

  8. NOAA NDBC SOS - waves

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA NDBC SOS server is part of the IOOS DIF SOS Project. The stations in this dataset have waves data. Because of the nature of SOS requests, requests for data...

  9. Neocolagenização induzida pelo resurfacing com laser erbium:YAG isolado e associado a lifting cutâneo: estudo morfométrico comparativo em ratos Comparison of single erbium:YAG laser resurfacing to a combination with cutaneous lifting: a morphometric study of neocollagenization in rats

    Directory of Open Access Journals (Sweden)

    Lucia Noronha

    2005-10-01

    Full Text Available INTRODUÇÃO: Diferente do lifting, cuja tração mecânica é a responsável pelo efeito clínico de rejuvenescimento sobre rugas profundas, a fibroplasia (ou neocolagenização é a responsável direta pelo resultado final da ação do laser sobre a pele com rugas superficiais, conferindo-lhe aparência mais jovem. O uso combinado dessas duas técnicas pode ser vantajoso, pois permite um resultado estético melhor com um único procedimento anestésico e cirúrgico em um curto período de recuperação. OBJETIVOS: O presente estudo morfométrico se propõe a avaliar se ocorre alguma alteração na espessura da fibroplasia induzida pelo resurfacing a laser erbium:YAG quando este se associa ao lifting cutâneo. MÉTODO: Foram utilizados 50 ratos da linhagem Wistar, divididos em dois grupos de 25, sendo que o primeiro grupo foi submetido à aplicação exclusiva de laser erbium:YAG no dorso de cada animal e o outro sofreu a aplicação de laser Erbium: YAG combinada ao lifting, o qual foi representado, no animal de experimentação, por retalho cutâneo dorsal pediculado. A fibroplasia foi avaliada nos dois grupos com medidas morfométricas lineares realizadas após o sacrifício dos animais nos dias 14, 28, 56, 84 e 112 do pós-operatório. RESULTADO: Foi observado aumento da fibroplasia em ambos os grupos estudados, porém o crescimento do colágeno foi superior no grupo submetido à terapia isolada com laser Erbium: YAG. CONCLUSÃO: A espessura da fibroplasia induzida pelo resurfacing a laser Erbium: YAG foi influenciada pela associação de um segundo procedimento cirúrgico no mesmo tempo operatório, neste caso, o lifting cutâneo.INTRODUCTION: The fibroplasia is the responsible for the final aesthetic results induced by laser resurfacing upon skin with superficial wrinkles. On the other hand, the lifting is responsible for the deeper wrinkles removal, produced by mechanic results. The use of the combination of these two rejuvenation

  10. Miopia aguda induzida por topiramato: relato de dois casos

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    Kathy Dadam Sgrott

    2011-06-01

    Full Text Available RESUMO A miopia aguda pode ser desencadeada pelo uso de medicações sistêmicas, dentre elas, o anticonvulsivante topiramato. Este trabalho descreve dois casos de pacientes jovens com quadro agudo bilateral de miopia induzida por terapia com topiramato para controle de síndrome depressiva, fazendo relação com casos semelhantes descritos na literatura e revisão bibliográfica pertinente.

  11. Redistribuição da gordura corporal induzida pelos inibidores de protease em pacientes com Aids Redistribution of body fat induced by HIV protease inhibitors in patients with AIDS

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    Cristina Mansur

    2006-10-01

    Full Text Available Apresentam-se quatro pacientes com infecção pelo vírus da imunodeficiência humana em tratamento com inibidores de proteases há oito meses e três semanas em média. Ressaltam-se o acúmulo de gordura na região dorsocervical e fáscies de lua cheia, semelhante à que ocorre na síndrome de Cushing.Four patients with Human Immunodeficiency Virus Infection in treatment with protease inhibitors for an average of 8 months and 3 weeks are reported. Fat accumulation in the cervical-dorsal region (buffalo hump and moon face, similar to that of Cushing's Syndrome, are highlighted.

  12. Efeito da dexmedetomidina sobre a arritmia cardíaca induzida pela adrenalina em cães anestesiados pelo sevofluorano Effect of dexmedetomidine on the heart arrhythmia induced by the adrenaline in dogs anesthetized by sevoflurane

    Directory of Open Access Journals (Sweden)

    V.F. Barbosa

    2007-12-01

    Full Text Available Avaliou-se o efeito da dexmedetomidina sobre o ritmo cardíaco em 20 cães, sem raça definida, de ambos os sexos e considerados sadios, anestesiados pelo sevofluorano e submetidos a doses crescentes de adrenalina. Os animais foram, aleatoriamente, distribuídos em dois grupos (placebo e dexmedetomidina. No grupo placebo, os animais receberam, por via intravenosa, solução de NaCl a 0,9%, na dose de 0,3ml/kg. Foram considerados dois momentos, M0 e M1, imediatamente antes e após a aplicação, respectivamente. Após 10 minutos, realizou-se a indução anestésica com sevofluorano, por meio de máscara facial vedada, até a perda do reflexo laringotraqueal. Em seguida, procedeu-se à intubação orotraqueal e a manutenção da anestesia foi realizada com a administração de sevofluorano na concentração de 1,5CAM, em circuito anestésico com reinalação parcial de gases. Decorridos 20 minutos da indução anestésica, iniciou-se a administração intravenosa contínua de solução de adrenalina a 2% em doses crescentes de 1, 2, 3, 4 e 5mg/kg/min, por meio de bomba de infusão, com aumento da dose em intervalos de 10 minutos. Imediatamente antes desse acréscimo eram feitas as mensurações (M2 a M6. No grupo dexmedetomidina empregou-se a mesma metodologia substituindo-se a solução de NaCl a 0,9% por hidrocloridrato de dexmedetomidina, na dose de 1µg/kg. Foram registradas as pressões arteriais, em M0 e em M2 a M6, e o traçado eletrocardiográfico, na derivação DII (M2 a M6, considerando-se para efeito estatístico o número total de bloqueios atrioventriculares (BAV de primeiro e segundo graus e de complexos ventriculares prematuros (ESV, coincidentes com cada dose de adrenalina. Os dados foram submetidos à análise de variância seguida pelo teste de Tukey (PThe effect of dexmedetomidine on the cardiac rhythm in twenty healthy mongrel dogs of both sexes anesthetized with sevofluorane and submitted to increasing doses of adrenaline was

  13. Métodos diagnósticos para retinopatia induzida pelo difosfato de cloroquina nos portadores de lúpus eritematoso sistêmico Diagnostic methods for chloroquine diphosphate induced retinopathy in systemic lupus erythematosus

    Directory of Open Access Journals (Sweden)

    Luciana Duarte Rodrigues

    2009-06-01

    Full Text Available OBJETIVOS: Avaliar diferentes métodos diagnósticos para a avaliação de pacientes portadores de lúpus eritematoso sistêmico, usuários crônicos do difosfato de cloroquina (DFC e, portanto, com alto risco para retinopatia tóxica. MÉTODOS: Foram analisados 72 olhos de 36 pacientes consecutivos, seguidos no Serviço de Reumatologia do Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, de julho de 2007 a abril de 2008. Dados demográficos e clínicos foram avaliados com o intuito de estudar os fatores de alto risco e comparar os seguintes métodos oftalmológicos: acuidade visual, biomicroscopia da córnea, biomicroscopia do fundo, retinografia, angiofluoresceinografia da retina, campo visual macular com mira branca. RESULTADOS: Dos 36 pacientes, 34 (94,4% eram mulheres. A média de idade foi 39,9 ± 9,8 anos, com tempo de doença igual a 13,9 ± 6,6 anos. Além do uso crônico da cloroquina, os pacientes apresentaram altas doses diárias (>3 mg/kg e cumulativas. Não foi observada relação entre estes fatores de alto risco e maior prevalência de retinopatia. Foi encontrada prevalência de retinopatia igual a 38,9%, confirmada por alterações bilaterais, centrais ou paracentrais e reprodutíveis no exame de campo visual. Outros exames indicados para seguimento, como acuidade visual, biomicroscopia de fundo e angiofluoresceinografia não foram capazes de diagnosticar a maioria das alterações confirmadas pelo campo visual. CONCLUSÃO: Foi observada alta prevalência de retinopatia por cloroquina entre os pacientes com alto risco, usuários crônicos do DFC, segundo os achados do campo visual. A avaliação desses pacientes deve considerar a realização do exame de campo visual em intervalos menores que os propostos, mesmo quando não há suspeita clínica.PURPOSE: To evaluate different diagnostic methods for high risk chloroquine retinopathy due to prolonged use of chloroquine (more than 5 years by systemic

  14. Rastreamento e seguimento dos portadores das lesões anais induzidas pelo papilomavírus humano como prevenção do carcinoma anal Screening and follow-up of patients with anal HPV induced lesions for anal carcinoma prevention

    Directory of Open Access Journals (Sweden)

    Sidney Roberto Nadal

    2009-06-01

    Full Text Available O Papilomavírus humano (HPV é o agente sexualmente transmissível mais comum na região perianal. O vírus provoca lesões clínicas e subclínicas que podem evoluir para carcinoma anal. É descrito o aumento da incidência desse tipo de tumor naqueles que praticam sexo anal; nos portadores, de ambos os sexos, de lesões genitais HPV induzidas; nas pessoas com neoplasias intraepiteliais anais de alto grau, o precursor do carcinoma, com maior incidência nos infectados pelo vírus da imunodeficiência humana (HIV, e com outras causas de supressão imunológica. Outra característica das lesões HPV induzidas é a elevada incidência de recidivas. Daí, a importância do seguimento por longo prazo e da pesquisa de meios terapêuticos para reduzir essa ocorrência. A possibilidade da detecção das lesões precursoras indica que programas padronizados de rastreamento para a prevenção do câncer anal deveriam ser instituídos. Os esfregaços anais para citologia vêm sendo realizados, com eficácia semelhante a das coletas cervicais e a colposcopia anal tem sido indicada para biópsias dirigidas quando a citologia mostrou-se alterada, embora muitos recomendam-na, também, como método de rastreamento. Nesse artigo, descrevemos a padronização da coleta de material para citologia anal e o método de realização da colposcopia anal, bem como a periodicidade com que devem ser repetidos.The human papillomavirus is the most frequent sexually transmitted agent in anorectal area. This virus provokes clinical and sub-clinical lesions that can evolve to anal carcinoma. Its incidence is increasing among those who practice anal receptive sex; in both gender patients with genital HPV induced lesions; in those with high grade anal intra-epithelial neoplasia, anal carcinoma precursor, mainly among HIV infected persons or with other causes of immunodeficiency. Another HPV induced lesions characteristic is their elevated incidence of recurrences. Therefore

  15. Estudo reológico da gelificação induzida pelo calor de proteínas do soro do leite e dos géis resultantes sob condições variadas de pH Rheological characterization of the heat-induced gelation of whey proteins and of the resulting gels under varied pH conditions

    Directory of Open Access Journals (Sweden)

    Cristina T. Andrade

    2005-06-01

    Full Text Available Medidas reólogicas sob cisalhamento oscilatório foram realizadas em reômetro de tensão e deformação controladas com suspensões de concentrado de proteínas do soro do leite (WPC a 10% (m/m em água e a diferentes condições de pH (pH 4,0, 4,6 e 7,0. O processo de gelificação induzida pelo calor foi investigado, assim como as propriedades viscoelásticas dos géis formados a 80°C e daqueles formados após o decréscimo da temperatura a 20°C. Foi verificado que, em presença de teores significativos de sais, procedentes do próprio soro, a concentração usada nos experimentos foi suficiente para a formação de géis macroscópicos, e que o pH exerce papel importante na formação e na natureza estrutural dos géis.Rheological measurements have been carried out in a controlled stress and strain rheometer on a whey proteins concentrate (WPC at 10% (w/w in water and at different pH conditions (pH 4.0, 4.6 and 7.0. The heat-induced gelation process was investigated, as well as the viscoelastic properties of the gels formed at 80°C and those formed after decreasing the temperature to 20°C. The results indicated that, in the presence of significant contents of salts from the whey sample, the concentration used in the experiments was sufficient for the formation of macroscopic gels, and that the pH exerts an important role on the formation and on the structural nature of the gels.

  16. Mediadores envolvidos na resposta febril induzida pela RANTES

    OpenAIRE

    Renes de Resende Machado

    2009-01-01

    Em estudo anterior, observamos que o Met-RANTES, antagonista de receptores CCR1 e CCR5 para quimiocinas, injetado pela via endovenosa (i.v.) reduziu a resposta febril induzida pelo lipopolissacarídeo (LPS) de E. coli, demonstrando o envolvimento da quimiocina RANTES (Regulada sob ativação, expressa e secretada por células T normais) nesta resposta. Além disso, a injeção intrahipotalâmica (i.h.) da RANTES dose-dependentemente aumentou a temperatura corporal de ratos, o qual foi caracterizado c...

  17. Ras activation by SOS

    DEFF Research Database (Denmark)

    Iversen, Lars; Tu, Hsiung-Lin; Lin, Wan-Chen

    2014-01-01

    Activation of the small guanosine triphosphatase H-Ras by the exchange factor Son of Sevenless (SOS) is an important hub for signal transduction. Multiple layers of regulation, through protein and membrane interactions, govern activity of SOS. We characterized the specific activity of individual ...

  18. Alteração da relação testosterona: cortisol induzida pelo treinamento de força em mulheres Alteración de la relación testosterona: cortisol inducida por el entrenamiento de fuerza en mujeres Alteration of testosterone: cortisol ratio induced by resistance training in women

    Directory of Open Access Journals (Sweden)

    Marco Carlos Uchida

    2004-06-01

    Full Text Available A razão entre a concentração de testosterona e cortisol (T:C é freqüentemente utilizada como indicativo do nível de estresse imposto pelo exercício. Alterações na concentração destes hormônios são responsáveis por modular diversas respostas induzidas pelo treinamento, como hipertrofia e ganho de força. O objetivo do presente estudo foi examinar a influência do protocolo de treinamento de força, conhecido como múltiplas-séries (MS, sobre o ganho de força, de resistência muscular localizada e a relação entre a concentração de hormônios catabólicos (cortisol e anabólicos (testosterona. Para testar esta hipótese cinco jovens do sexo feminino com um ano de experiência em treinamento de força foram submetidas ao protocolo MS. As amostras de sangue foram coletadas antes e imediatamente após o exercício, no primeiro dia e após oito semanas de treinamento. Os testes de 1-RM e de repetições máximas foram realizados também no início e ao final das oito semanas de treinamento de força. Não foram observadas alterações na massa corporal, no IMC, na percentagem de massa gorda e na força máxima (1-RM no supino, no agachamento e na rosca direta. O número de repetições máximas a 50% de 1-RM foi aumentado apenas para o supino (p La razón entre testosterona y cortisol (T:C es frecuentemente utilizada como indicador del nivel de stress impuesto por el ejercicio. Las alteraciones de las concentraciones de estas hormonas son las responsables por modular diversas respuestas inducidas por el entrenamiento, como son la hipertrofia y el aumento de la fuerza. El objetivo del presente estudio fué examinar la influencia del protocolo de entrenamiento de fuerza, conocido como series multiples (MS, sobre la ganancia de fuerza, la resistencia muscular localizada y la relación entre las concentraciones de las hormonas catabólicas (cortisol y anabólicas (testoterona. Para testar esta hipótesis, cinco jovenes del sexo feminino

  19. Exercício físico previne alterações cardiometabólicas induzidas pelo uso crônico de glicocorticóides Ejercicio físico previene alteraciones cardiometabólicas inducidas por el uso crónico de glucocorticoides Exercise prevents cardiometabolic alterations induced by chronic use of glucocorticoids

    Directory of Open Access Journals (Sweden)

    Carlos Hermano da Justa Pinheiro

    2009-10-01

    Full Text Available FUNDAMENTO: Cronicamente, os glicocorticóides induzem alterações cardiometabólicas adversas, incluindo resistência à insulina, diabete, dislipidemia, esteatose hepática e hipertensão arterial. OBJETIVOS: Avaliar o efeito da prática regular de exercício físico aeróbio sobre as alterações cardiometabólicas induzidas por administração crônica de dexametasona (Dex - 0,5 mg/kg/dia i.p em ratos. MÉTODOS: Ratos Wistar machos (n = 24 foram divididos em quatro grupos: Grupo controle; Grupo treinado; Grupo tratado com Dex e Grupo tratado com Dex e treinado. O treinamento físico (iniciado 72 horas após a primeira dose de Dex foi realizado 3 vezes por semana, até o final do tratamento. Ao final desse período, realizaram-se as seguintes avaliações bioquímicas: glicemia em jejum, teste de tolerância à glicose e análise do perfil lipídico no sangue que incluiu colesterol total (CT, LDL-c, HDL-c, VLDL-c e triglicerídeos (TG. O peso do músculo gastrocnêmio, análise histopatológica do fígado e os índices cardiometabólicos (CT/HDL-c, LDL-c/HDL-c e TG/HDL-c também foram avaliados. RESULTADOS: Observou-se hiperglicemia, menor tolerância à glicose, elevação do CT, LDL-c, VLDL-c e TG, diminuição do HDL-c, presença de esteatose hepática, hipotrofia muscular e elevação dos índices CT/HDL-c, LDL-c/HDL-c e TG/HDL-c nos animais tratados com Dex. O exercício físico reduziu a hiperglicemia, melhorou a tolerância à glicose, reduziu a dislipidemia e preveniu a esteatose hepática , a hipotrofia muscular e reduziu os índices CT/HDL-c, LDL-c/HDL-c e TG/HDL-c. Entretanto, não houve efeito significante do treinamento físico sobre o HDL-c. CONCLUSÃO: O exercício físico aeróbio tem efeito protetor contra as alterações cardiometabólicas induzidas pelo uso crônico de glicocorticóides.FUNDAMENTO: Crónicamente, los glucocorticoides inducen alteraciones cardiometabólicas adversas, incluyendo resistencia a la insulina

  20. Avaliação da hiperalgesia e alterações histológicas do gânglio da raiz dorsal induzidas pelo núcleo pulposo Evaluation of hyperalgesia and histological changes of dorsal root ganglion induced by nucleus pulposus

    Directory of Open Access Journals (Sweden)

    André Luiz de Souza Grava

    2010-01-01

    Full Text Available OBJETIVO: Avaliar a hiperalgesia e as alterações histológicas induzidas pelo contato do gânglio da raiz dorsal com o núcleo pulposo. MÉTODOS: Foram utilizados 20 ratos Wistar, divididos em dois grupos experimentais. Em um dos grupos um fragmento do NP autólogo retirado da região sacrococcígea foi colocado sobre os gânglios da raiz dorsal de L5, e no outro grupo (controle um fragmento de tecido adiposo foi colocado sobre o gânglio da raiz dorsal de L5. A hiperalgesia mecânica e térmica foi avaliada no terceiro dia, na primeira, terceira, quinta e sétima semanas de pós-operatório. O gânglio da raiz dorsal de L5 foi retirado na primeira, terceira, quinta e sétima semanas de pós-operatório para estudo histológico por meio da coloração com HE e estudo histoquímico (marcação específica para iNOS. RESULTADOS: Foi observada hiperalgesia térmica e mecânica de maior intensidade no grupo de animais em que o NP foi colocado em contato com o GRD, e nesse grupo o estudo histológico evidenciou alterações dos tecidos do gânglio da raiz dorsal, caracterizadas por processo inflamatório e degeneração axonal. As alterações histopatológicas dos tecidos do gânglio da raiz dorsal apresentaram intensidade crescente com o aumento do período de observação, apresentando correlação com a manutenção da hiperalgesia observada na avaliação comportamental. A imunohistoquímica com marcação específica para iNOS demonstrou, no grupo de animais em que o núcleo pulposo foi colocado em contato com o gânglio da raiz dorsal, aumento da expressão dessa enzima nos núcleos das células inflamatórias (células da glia ao redor dos neurônios. CONCLUSÃO: O contato do núcleo pulposo com o gânglio da raiz dorsal de L5 induziu a hiperalgesia mecânica e térmica e provocou alterações histológicas dos componentes do gânglio da raiz dorsal. As alterações foram caracterizadas por processo inflamatório e degenerativo das

  1. Alterações em variáveis motoras e metabólicas induzidas pelo treinamento durante um macrociclo em jogadores de handebol Alteraciones en las variables motoras y metabólicas inducidas por el entrenamiento durante un macro ciclo en jugadores de balonmano Changes in metabolic and motor performance variables induced by training in handball players

    Directory of Open Access Journals (Sweden)

    Juvenilson de Souza

    2006-06-01

    Full Text Available A monitoração do treinamento é uma prática comum no desporto. Essa monitoração deve ser baseada em testes específicos e que reflitam as adaptações às diversas etapas de treinamento, permitindo, assim, ajustes no programa deste. Assim, este estudo teve como objetivo analisar as alterações em variáveis motoras e metabólicas induzidas pelo treinamento durante um macrociclo em jogadores de handebol. Os atletas foram submetidos a um programa de preparação fundamentado no modelo de periodização proposto por Verkhoshanski(7 e adaptado por Oliveira(8. Foram estudados 11 jogadores de handebol, que realizaram treinamentos diários, na faixa etária de 20 a 32 anos, massa corporal média de 89,5 ± 10,4kg (70,2 a 105,1kg, e estatura média de 184,4 ± 6,7cm (171,8 a 198cm, filiados à equipe "UniFil/Londrina" do município de Londrina - PR. Os handebolistas foram submetidos a duas baterias de testes: a primeira no início do segundo macrociclo de treinamento e a segunda após 16 semanas, antecedendo o início da liga nacional. Para análise dos dados foi utilizado teste t para medidas repetidas com p La monitorización del entrenamiento es una práctica común en el deporte. Esa monitorización debe basarse en pruebas específicas y que reflejen las adaptaciones a las diversas etapas del entrenamiento, permitiendo así, ajustes en el programa del mismo. Así, este estudio ha tenido como objetivo analizar las alteraciones en las variables motoras y metabólicas inducidas por el entrenamiento durante un macro ciclo en jugadores de balonmano. Los atletas fueron sometidos a un programa de preparación basado en el modelo de periodicidad propuesto por Verkhoshanski(7 y adaptado por Oliveira(8. Fueron estudiados 11 jugadores de balonmano que realizaron entrenamiento diario, con edades entre 20 y 32 años, masa corporal media de 89,5 ± 10,4 kg (70,2 y 105,1 kg, y estatura media de 184,4 ± 6,7 cm (171,8 y 198 cm, afiliados al equipo "Uni

  2. NOAA NOS SOS, EXPERIMENTAL - Currents

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA NOS SOS server is part of the IOOS DIF SOS Project. The stations in this dataset have currents data. *These services are for testing and evaluation use...

  3. NOAA NOS SOS, EXPERIMENTAL - Wind

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA NOS SOS server is part of the IOOS DIF SOS Project. The stations in this dataset have wind data. *These services are for testing and evaluation use only*...

  4. Collection for SOS animaux

    CERN Multimedia

    2005-01-01

    The Pays de Gex animal shelter is collecting funds. There will be things to buy. You will be able to make a donation and/or become a member of the association or simply get information. SOS Animaux stall (Hall, Build. 60, next to restaurant 1) On Wednesday 23 November 2005 (from 9h - 17h non-stop)

  5. SOS-projektet

    DEFF Research Database (Denmark)

    Blomhøj, Morten; Jensen, Tomas Højgaard

    2007-01-01

    Artiklen beretter om og analyserer det såkaldte SOS-projekt, hvor matematiklærere fra grundskolen, gymnasiet og læreruddannelsen har samarbejdet med matematikdidaktiske forskere om at undersøge og afhjælpe nogle af de udfordringer som danske elever møder i matematik ved overgangen fra grundskole...

  6. CMOS/SOS processing

    Science.gov (United States)

    Ramondetta, P.

    1980-01-01

    Report describes processes used in making complementary - metal - oxide - semiconductor/silicon-on-sapphire (CMOS/SOS) integrated circuits. Report lists processing steps ranging from initial preparation of sapphire wafers to final mapping of "good" and "bad" circuits on a wafer.

  7. NOAA NDBC SOS, 2006-present, winds

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA NDBC SOS server is part of the IOOS DIF SOS Project. The stations in this dataset have winds data. Because of the nature of SOS requests, requests for data...

  8. NOAA NDBC SOS, 2007-present, currents

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA NDBC SOS server is part of the IOOS DIF SOS Project. The stations in this dataset have currents data. Because of the nature of SOS requests, requests for...

  9. The meaning of ordered SOS

    NARCIS (Netherlands)

    Mousavi, M.R.; Phillips, I.C.C.; Reniers, M.A.; Ulidowski, I.; Arun-Kumar, S.; Garg, N.

    2006-01-01

    Structured Operational Semantics (SOS) is a popular method for defining semantics by means of deduction rules. An important feature of deduction rules, or simply SOS rules, are negative premises, which are crucial in the definitions of such phenomena as priority mechanisms and time-outs. Orderings

  10. [SOS-repair--60 years].

    Science.gov (United States)

    Zavil'gel'skiĭ, G B

    2013-01-01

    This review integrates 60 years of research on SOS-repair and SOS-mutagenesis in procaryotes and eucaryotes, from Jean Weigle experiment in 1953 year (mutagenesis of lambda bacteriophage in UV-irradiated bacteria) to the latest achievements in studying SOS-mutagenesis on all living organisms--Eukarya, Archaea and Bacteria. A key role in establishing of a biochemical basis for SOS-mutagenesis belonges to the finding in 1998-1999 years that specific error-prone DNA polymerases (PolV and others) catalysed translesion synthesis on damaged DNA. This review focuses on recent studies addressing the new models for SOS-induced mutagenesis in Escherichia coli and Home sapiens cells.

  11. NOAA NOS SOS, EXPERIMENTAL, 1902-present, Salinity

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA NOS SOS server is part of the IOOS DIF SOS Project. The stations in this dataset have salinity data. *These services are for testing and evaluation use...

  12. Vesiculation from Pseudomonas aeruginosa under SOS.

    Science.gov (United States)

    Maredia, Reshma; Devineni, Navya; Lentz, Peter; Dallo, Shatha F; Yu, Jiehjuen; Guentzel, Neal; Chambers, James; Arulanandam, Bernard; Haskins, William E; Weitao, Tao

    2012-01-01

    Bacterial infections can be aggravated by antibiotic treatment that induces SOS response and vesiculation. This leads to a hypothesis concerning association of SOS with vesiculation. To test it, we conducted multiple analyses of outer membrane vesicles (OMVs) produced from the Pseudomonas aeruginosa wild type in which SOS is induced by ciprofloxacin and from the LexA noncleavable (lexAN) strain in which SOS is repressed. The levels of OMV proteins, lipids, and cytotoxicity increased for both the treated strains, demonstrating vesiculation stimulation by the antibiotic treatment. However, the further increase was suppressed in the lexAN strains, suggesting the SOS involvement. Obviously, the stimulated vesiculation is attributed by both SOS-related and unrelated factors. OMV subproteomic analysis was performed to examine these factors, which reflected the OMV-mediated cytotoxicity and the physiology of the vesiculating cells under treatment and SOS. Thus, SOS plays a role in the vesiculation stimulation that contributes to cytotoxicity.

  13. NOAA NOS SOS, EXPERIMENTAL, 1902-present, Conductivity

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA NOS SOS server is part of the IOOS DIF SOS Project. The stations in this dataset have conductivity data. *These services are for testing and evaluation use...

  14. A Modular SOS for Action Notation - Revisited

    DEFF Research Database (Denmark)

    Mosses, Peter David

    A draft modular SOS for the new version of AN, referred to as AN-2, has been available since 2000. It is written in CASL and has been checked for well-formedness using CATS (CASL Tool Set). It appears to be significantly more accessible than the original SOS of AN-1. However, it now appears......-notation for the modular SOS rules. After discussing the issues, we look at some illustrative examples taken from an improved modular SOS of AN-2 (in preparation). We also look at the possibility of empirical testing of the modular SOS by a straightforward translation to Prolog....

  15. Congruence for SOS with data

    NARCIS (Netherlands)

    Mousavi, M.R.; Reniers, M.A.; Groote, J.F.

    2004-01-01

    Abstract While studying the specification of the operational semantics of different programming languages and formalisms, one can observe the following three facts. Firstly, Plotkin¿s style of Structured Operational Semantics (SOS) has become a standard in defining operational semantics. Secondly,

  16. Mutational specificity of SOS mutagenesis

    International Nuclear Information System (INIS)

    Kato, Takeshi

    1986-01-01

    In an approach to the isolation of mutants of E. coli unable to produce mutations by ultraviolet light, the author has found new umuC-mutants. Their properties could be explained by ''SOS hypothesis of Radman and Witkin'', which has now been justified by many investigators. Analysis of the umuC region of E. coli chromosome cloned in pSK 100 has led to the conclusion that two genes, umuD and umuC, having the capacity of mutation induction express in the same mechanism as that of SOS genes, which is known to be inhibited by LexA protein bonding to ''SOS box'' found at promotor region. Suppressor analysis for mutational specificity has revealed: (i) umuDC-independent mutagens, such as EMS and (oh) 4 Cy, induce selected base substitution alone; and (ii) umuDC-dependent mutagens, such as X-rays and gamma-rays, induce various types of base substitution simultaneously, although they have mutational specificity. In the umuDC-dependent processes of basechange mutagenesis, the spectra of base substitution were a mixture of base substitution reflecting the specific base damages induced by individual mutagens and nonspecific base substitution. In conclusion, base substitution plays the most important role in umuDC-dependent mutagenesis, although mutagenesis of umuDC proteins remains uncertain. (Namekawa, K.)

  17. NOAA NDBC SOS, 2007-present, sea_water_practical_salinity

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA NDBC SOS server is part of the IOOS DIF SOS Project. The stations in this dataset have sea_water_practical_salinity data. Because of the nature of SOS...

  18. NOAA NDBC SOS, 2006-present, sea_water_temperature

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA NDBC SOS server is part of the IOOS DIF SOS Project. The stations in this dataset have sea_water_temperature data. Because of the nature of SOS requests,...

  19. Miopização Aguda induzida pelo Topiramato – caso clínico

    Directory of Open Access Journals (Sweden)

    Silvestre Cruz

    2016-06-01

    Full Text Available O topiramato é um fármaco antiepilético usado no tratamento da epilepsia, estabilização do humor e prevenção da enxaqueca. Habitualmente bem tolerado, está associado pouco frequentemente a efeitos secundários oftalmológicos, tais como miopização e glaucoma agudos. É relatado o caso clinico de uma adolescente de 17anos, medicada com topiramato, que recorreu ao serviço de urgência por diminuição súbita da acuidade visual (AV bilateral. No exame inicial constatou-se miopização e a biomicroscopia ultrassónica (UBM revelou efusão uveal e edema do corpo ciliar com anteriorização do diafragma iris-cristalino e estreitamento angular bilateral, tendo sido suspenso o topiramato. Um mês após a interrupção a doente demonstrou reversão do quadro para emetropização e normalização das estruturas anatómicas, confirmada pela UBM. Os autores pretendem alertar para as complicações oftalmológicas de um fármaco amplamente usado, ressaltando a importância de um diagnóstico precoce evitando graves consequências, nomeadamente evolução para glaucoma agudo e potencial perda da AV.

  20. Clorose férrica induzida pelo calcário

    Directory of Open Access Journals (Sweden)

    Maribela Pestana

    2014-12-01

    Full Text Available A clorose férrica é um dos problemas nutricionais mais frequentes e difíceis de controlar nas plantas cultivadas em solos calcários. Nesses solos de reação alcalina, que ocupam cerca de um terço da superfície terreste, prevalece o íon bicarbonato, identificado como sendo um dos principais fatores indutores da clorose férrica. Como resultado, a alcalinidade dificulta a biodisponibilidade do Fe na solução do solo, a redução e a absorção do Fe pela planta, assim como, o respectivo transporte e assimilação desse elemento no metabolismo vegetal. Face a esse desequilíbrio nutricional, as plantas apresentam diferentes estratégias de resposta, que, nessas condições, nem sempre são eficientes. Consequentemente, a produção, a qualidade do fruto e a data de colheita são negativamente afetados. Os gastos com a prevenção e a correção da clorose férrica são elevados e inevitáveis, para assegurar a viabilidade e a sustentabilidade da produção agrícola, nas áreas em que se conjugam a presença de carbonato de cálcio no solo e outros fatores, como os climas áridos ou semiáridos. Com este trabalho, pretende-se resumir a dinâmica do ferro nos solos calcários e suas consequências no metabolismo do Fe e na produção agrícola, com destaque para os frutos.

  1. Blefarite e tricomegalia induzidas pelo cetuximabe Blepharitis and trichomegaly induced by cetuximab

    Directory of Open Access Journals (Sweden)

    Paulo Ricardo Criado

    2010-12-01

    Full Text Available Nós descrevemos uma mulher de 41 anos em tratamento de câncer colorretal avançado que, após a segunda dose de cetuximabe, desenvolveu intensa blefarite e tricomegalia bilateral. A toxicidade ocular decorrente do cetuximabe tem sido relatada, porém ainda tem mecanismos fisiopatogênicos incertos.This report describes the case of a 41-year old woman in treatment for advanced colorectal cancer who developed severe bilateral blepharitis and trichomegaly after the second dose of cetuximab. Cetuximab-related eyelid toxicity has been described previously; however, its pathogenesis has not yet been clearly established.

  2. Pneumonia Organizativa Fibrinosa Aguda Induzida pelo Rituximab: Uma Entidade a (Reconhecer

    Directory of Open Access Journals (Sweden)

    Alexandra Couto

    2017-10-01

    Dada a utilização crescente de terapêuticas-alvo em oncologia, o presente caso pretende contribuir para o reconhecimento desta entidade como potencial evento adverso. Recebido: 07/06/2017 - Aceite: 30/08/2017

  3. The Arabidopsis SOS2 protein kinase physically interacts with and is activated by the calcium-binding protein SOS3

    OpenAIRE

    Halfter, Ursula; Ishitani, Manabu; Zhu, Jian-Kang

    2000-01-01

    The Arabidopsis thaliana SOS2 and SOS3 genes are required for intracellular Na+ and K+ homeostasis and plant tolerance to high Na+ and low K+ environments. SOS3 is an EF hand type calcium-binding protein having sequence similarities with animal neuronal calcium sensors and the yeast calcineurin B. SOS2 is a serine/threonine protein kinase in the SNF1/AMPK family. We report here that SOS3 physically interacts with and activates SOS2 protein kinase. Genetically, sos2sos3 double mutant analysis ...

  4. Prototyping SOS meta-theory in Maude

    NARCIS (Netherlands)

    Mousavi, M.R.; Reniers, M.A.; Mosses, P.D.; Ulidowski, I.

    2006-01-01

    We present a prototype implementation of SOS meta-theory in the Maude term rewriting language. The prototype defines the basic concepts of SOS meta-theory (e.g., transition formulae, deduction rules and transition system specifications) in Maude. Besides the basic definitions, we implement methods

  5. Semantics and expressiveness of ordered SOS

    NARCIS (Netherlands)

    Mousavi, M.R.; Phillips, I.C.C.; Reniers, M.A.; Ulidowski, I.

    2009-01-01

    Structured Operational Semantics (SOS) is a popular method for defining semantics by means of transition rules. An important feature of SOS rules is negative premises, which are crucial in the definitions of such phenomena as priority mechanisms and time-outs. However, the inclusion of negative

  6. Bacterial SOS response: a food safety perspective

    NARCIS (Netherlands)

    Veen, van der S.; Abee, T.

    2011-01-01

    The SOS response is a conserved inducible pathway in bacteria that is involved in DNA repair and restart of stalled replication forks. Activation of the SOS response can result in stress resistance and mutagenesis. In food processing facilities and during food preservation, bacteria are exposed to

  7. Lesão renal aguda induzida por contraste: importância dos critérios diagnósticos para estabelecer a prevalência e o prognóstico na unidade de terapia intensiva

    Directory of Open Access Journals (Sweden)

    Edmilson Leal Bastos de Moura

    2017-09-01

    Full Text Available RESUMO Objetivo: Estabelecer se há superioridade entre os critérios para predizer desfecho clínico desfavorável na lesão renal aguda e nefropatia induzidas por contraste. Métodos: Estudo retrospectivo conduzido em hospital terciário com 157 pacientes submetidos à infusão de contraste radiológico para fins propedêuticos. Resultados: Cumpriram os critérios para inclusão 147 pacientes. Aqueles que cumpriram os critérios de lesão renal aguda induzida por contraste (59 também cumpriram os critérios para nefropatia induzida por contraste (76; 44,3% dos pacientes cumpriram os critérios para o estadiamento pelo sistema KDIGO; 6,4% dos pacientes necessitaram utilizar terapia de substituição renal, e 10,7% dos pacientes morreram. Conclusão: O diagnóstico de nefropatia induzida por contraste foi o critério mais sensível para determinar a necessidade de terapia de substituição renal e óbito, enquanto o KDIGO demonstrou a maior especificidade; na população avaliada, não houve correlação entre o volume de contraste e a progressão para lesão renal induzida por contraste, nefropatia induzida por contraste, diálise de suporte ou óbito.

  8. Hepatotoxicidade Induzida por Fármacos Anti-tuberculosos

    Directory of Open Access Journals (Sweden)

    Jaime R. Ungo

    1998-11-01

    Full Text Available RESUMO: Com o objectivo de:Investigar a possível associação entre a infecção pelo virus da Hepatite C (VHC e/ou vírus da Imunodeficiência Humana (VIH com a ocorrência de hepatotoxicidade induzida pela medicação antibacilar.Investigar o possivel papel que o alfa-interferão 2a possa ter. permitindo a re-introdução da terapêutica anti-tuberculosa em doentes com infecção pelo VHC em que se observam clevações recorrentes dos niveis de transaminases enquanto submetidos a medicação com antibacilares.Procedeu-se a um estudo prospectivo em doentes internados no A. G. Holley Hospital com o diagnóstico de tuberculose, no período compreendido entre Dezembro de 1994 e Março de 1996. A população de doentes deste Hospital é essencialmente formada por doentes não aderentes à terapêutica em ambulatório ou doentes em que a existência de patologia associada, problemas psiquiátricos ou sociais interferem com a eficácia terapêutica em ambulatório.Os doentes eram submetidos a uma avaliarção analítica com determinação de ALT, AST, bilirrubina total, serologias para Hepatite A, B, C e VIH e doseamento urinário de drogas. Os exames para avaliação da funrção hepática eram determinados mensalmente após o inicio da medicação e, caso se verificassem altcrarções passavam a ser determinados com periodicidade bissemanal.Todos os doentes com infecção pelo VHC, VIH negativos, que apresentaram elevação recorrente das provas de funrção hepatica (P.F.H. após re-introdução da terapêutica antibacilar, çntraram no protocolo com alfa-interferão. Estes doentes eram submetidos a biópsia hepática e se nesta, se observasse inflamação activa. iniciavam terapêutlca com alfa-interferão. Uma vez que as P.F.H. normalizassem ou se verificasse uma melhoria de pelo menos 50%, sob terapêutica com interferao, re-introduzia-se sequencialmente terapêutica com isoniazida (INH, rifabutina (RBT e pirazinamida (PZA com determina

  9. SOS

    DEFF Research Database (Denmark)

    2015-01-01

    Dette støttepapir kan ikke stå alene. Teksten er alene tænkt som en hjælp til gruppearbejdet eller den enkelte midt i skriveprocessen og kan ikke anvendes, hvis man ikke er godt hjemme i grundbøgernes behandling af de samme emner. Det anbefales at følge undervisernes instrukser, anvisninger i gru...

  10. Efeito do dinitrato de isossorbida sobre a hiperalgesia corneana induzida por lipopolissacarídeo

    Directory of Open Access Journals (Sweden)

    Jayter Silva Paula

    2011-10-01

    Full Text Available OBJETIVO: Avaliar o efeito do dinitrato de isossorbida (DNI sobre a hiperalgesia corneana e a infiltração de neutrófilos em um modelo experimental de ceratite superficial induzida pelo lipopolissacarídeo (LPS. MÉTODOS: A hiperalgesia foi estudada através da indução de uma ceratite inflamatória em ratos (n = 60, pela exposição da córnea ao LPS (LPS +. Após a exposição, os olhos foram tratados durante quatro dias, com as soluções tópicas: DNI (200 mg, 65 mg e 20 mg, prednisolona 1% (PRED ou Veículo. Olhos controles foram expostos apenas à solução salina (LPS -. Para avaliar a dor ocular, contouse o número de piscadas em 40 segundos, após instilação de uma gota de capsaicina 0,01 mM. A análise histopatológica foi realizada para avaliação da infiltração neutrofílica. RESULTADOS: Foram observadas áreas esbranquiçadas no estroma corneano dos olhos com a ceratite induzida pelo LPS, entre os dias 3 e 15 dias. Esses olhos (LPS + apresentaram um número significativamente maior de piscadas que os LPS - (P = 0,019 à estimulação química. O tratamento tópico com o DNI reduziu o número de piscadas observadas (P = 0,010. Da mesma forma, olhos expostos ao LPS e tratados com DNI demonstraram redução significativa na infiltração neutrofílica (P = 0,0031. CONCLUSÃO: Baixas doses de DNI reduziram a hiperalgesia corneana e a infiltração neutrofílica nesse modelo de ceratite. Dessa forma, doadores de óxido nítrico, como o DNI, poderão ser úteis no futuro ao tratamento clínico de diversas condições dolorosas da superfície ocular.

  11. SNAP Operating System (SOS) user's guide

    International Nuclear Information System (INIS)

    Sabuda, J.D.; Polito, J.; Walker, J.L.; Grant, F.H. III.

    1982-03-01

    The SNAP Operating System (SOS) is a FORTRAN 77 program which provides assistance to the safeguards analyst who uses the Safeguards Automated Facility Evaluation (SAFE) and the Safeguards Network Analysis Procedure (SNAP) techniques. Features offered by SOS are a data base system for storing a library of SNAP applications, computer graphics representation of SNAP models, a computer graphics editor to develop and modify SNAP models, a SAFE-to-SNAP interface, automatic generation of SNAP input data, and a computer graphics postprocessor for SNAP. The SOS User's Guide is designed to provide the user with the information necessary to use SOS effectively. Examples are used throughout to illustrate the concepts. The format of the user's guide follows the same sequence as would be used in executing an actual application

  12. Terapia por contensão induzida: revisão de ensaios clínicos

    OpenAIRE

    Silva,Lidiane de Araújo; Tamashiro,Vanessa; Assis,Rodrigo Deamo

    2010-01-01

    INTRODUÇÃO: A Terapia por Contensão Induzida (TCI) é uma terapêutica que visa à recuperação da função do membro superior (MS) parético de pacientes com sequelas de um acidente vascular cerebral por meio de treinamento intensivo, uso de uma restrição no MS não-parético durante 90% do dia e pelo pacote de transferência. OBJETIVO: O objetivo deste trabalho é realizar uma revisão entre os estudos que compararem a TCI com outras terapêuticas. METODOLOGIA: Foi realizada uma pesquisa nas bases de da...

  13. Toxicidade pulmonar induzida pela rapamicina Lung toxicity induced by rapamycin

    Directory of Open Access Journals (Sweden)

    C Damas

    2006-11-01

    Full Text Available As doenças pulmonares induzidas por fármacos constituem uma causa crescente de morbilidade, tendo sido descritas diferentes formas de toxicidade associadas a inúmeras substâncias. O sirolimus (rapamicina é um fármaco imunossupressor usado de forma crescente no contexto do transplante de órgãos sólidos, nomeadamente no transplante renal. A toxicidade pulmonar tem sido descrita como um dos potenciais efeitos laterais, nomeadamente causando formas de pneumonite intersticial ou, mais raramente, hemorragia alveolar. Os autores descrevem os casos de quatro doentes (3 do sexo masculino, 1 do sexo feminino com idades compreendidas entre os 46-71 anos, recipientes de transplante renal (rim cadáver há 3 anos (1 doente e 7 anos (3 doentes. A imunosupressão consistia em micofenolato mofetil, prednisolona e rapamicina. Os quatro doentes foram admitidos por febre, tosse produtiva (2 e dispneia (3. Apresentavam imagem radiológica de infiltrados pulmonares bilaterais de predomínio basal. O LBA mostrou alveolite linfocítica em 3 doentes, tendo-se observado no entanto diferentes relações CD4/CD8., para além de neutrofilia em 2 deles. No restante doente, observou-se hemorragia alveolar grave. Não houve em nenhum dos casos qualquer isolamento de micro organismos patogénicos no LBA. As queixas apresentadas, bem como as alterações radiológicas regrediram com a suspensão do fármaco. Estes quatro casos revelaram alguma variedade, quer na apresentação clínica, quer nos achados dos exames subsidiários efectuados, nomeadamente no LBA. Este facto pode ter como causa diferentes mecanismos fisiopatológicos a nível do pulmão induzidos pelo sirolimus.Drug induced lung diseases (DILD are an increasingly cause of morbidity. Many drugs have been described, causing several patterns of injury. Sirolimus is an immunosuppressive agent increasingly used in renal and other solid organ transplantation. Pulmonary toxicity has been recognised as a potential

  14. Segregação mitótica induzida pelo edulcorante L-Aspartil-L-Fenilalanina-Metil-Ester (aspartame em células diplóides de Aspergillus nidulans - DOI: 10.4025/actascibiolsci.v25i1.2121 Mitotic segregation induced by edulcorant l-aspartyl-l-phenylalanine-metyl-ester (aspartame in diploid cells of Aspergillus nidulans - DOI: 10.4025/actascibiolsci.v25i1.2121

    Directory of Open Access Journals (Sweden)

    Marialba Avezum Alves Castro Prado

    2003-04-01

    Full Text Available O presente trabalho demonstra o efeito recombinagênico do edulcorante artificial L-Aspartil-L-Fenilalanina-Metil-Ester (aspartame, amplamente utilizado em dietas hipocalóricas e de baixo consumo de glicose. Este efeito pode refletir respostas celulares a danos genéticos induzidos pelo edulcorante artificial, no período G2 do ciclo celular e demonstra sua capacidade de induzir homozigose de genes recessivos previamente presentes em heterozigoseThis study aims to verify the recombinogenic effect of artificial edulcorant l-aspartil-l-phenylalanine-metyl-ester (aspartame, widely used in hypocaloric and low-glucose diets. Such effect may reflect cell response to genetic damage, induced by artificial edulcorant in the cell cycle G2 period and shows its capacity to induce homozygosis of recessive genes, previously present in heterozygosis

  15. NOAA NOS SOS, EXPERIMENTAL, 1853-present, Air Temperature

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA NOS SOS server is part of the IOOS DIF SOS Project. The stations in this dataset have air temperature data. *These services are for testing and evaluation...

  16. NOAA NOS SOS, EXPERIMENTAL, 1853-present, Barometric Pressure

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA NOS SOS server is part of the IOOS DIF SOS Project. The stations in this dataset have barometric pressure data. *These services are for testing and...

  17. NOAA NOS SOS, EXPERIMENTAL, 1853-present, Water Temperature

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA NOS SOS server is part of the IOOS DIF SOS Project. The stations in this dataset have water temperature data. *These services are for testing and evaluation...

  18. NOAA NOS SOS, EXPERIMENTAL, 1853-present, Water Level

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA NOS SOS server is part of the IOOS DIF SOS Project. The stations in this dataset have water surface height above a reference datum. *These services are for...

  19. Mapping Modular SOS to Rewriting Logic

    DEFF Research Database (Denmark)

    Braga, Christiano de Oliveira; Haeusler, Erik Hermann; Meseguer, José

    Modular SOS (MSOS) is a framework created to improve the modularity of structural operational semantics specifications, a formalism frequently used in the fields of programming languages semantics and process algebras. With the objective of defining formal tools to support the execution and verif......Modular SOS (MSOS) is a framework created to improve the modularity of structural operational semantics specifications, a formalism frequently used in the fields of programming languages semantics and process algebras. With the objective of defining formal tools to support the execution...

  20. Mapping Modular SOS to Rewriting Logic

    DEFF Research Database (Denmark)

    Braga, Christiano de Oliveira; Haeusler, Edward Hermann; Meseguer, José

    2003-01-01

    Modular SOS (MSOS) is a framework created to improve the modularity of structural operational semantics specifications, a formalism frequently used in the fields of programming languages semantics and process algebras. With the objective of defining formal tools to support the execution and verif......Modular SOS (MSOS) is a framework created to improve the modularity of structural operational semantics specifications, a formalism frequently used in the fields of programming languages semantics and process algebras. With the objective of defining formal tools to support the execution...

  1. A syntactic commutativity format for SOS

    NARCIS (Netherlands)

    Mousavi, M.R.; Reniers, M.A.; Groote, J.F.

    2005-01-01

    Considering operators defined using Structural Operational Semantics (SOS), commutativity axioms are intuitive properties that hold for many of them. Proving this intuition is usually a laborious task, requiring several pages of boring and standard proof. To save this effort, we propose a syntactic

  2. A hierarchy of SOS rule formats

    NARCIS (Netherlands)

    Groote, J.F.; Mousavi, M.R.; Reniers, M.A.; Mosses, P.D.; Ulidowski, I.

    2006-01-01

    In 1981 Structural Operational Semantics (SOS) was introduced as a systematic way to define operational semantics of programming languages by a set of rules of a certain shape [G.D. Plotkin. A structural approach to operational semantics. Technical Report DAIMI FN-19, Computer Science Department,

  3. SOS Children's Friendly Community Historical Overview

    Science.gov (United States)

    Lukaš, Mirko; Lenard, Ivan

    2014-01-01

    SOS Children's Village Croatia is categorized as a children's home whose primary goal is taking care of children without an adequate parental care or parents themselves. Moreover, it aims at providing children, regardless of their racial, national or religious affiliation, with affection and love in a safe family environment. In addition, SOS…

  4. Inhibitors of Ras-SOS Interactions.

    Science.gov (United States)

    Lu, Shaoyong; Jang, Hyunbum; Zhang, Jian; Nussinov, Ruth

    2016-04-19

    Activating Ras mutations are found in about 30 % of human cancers. Ras activation is regulated by guanine nucleotide exchange factors, such as the son of sevenless (SOS), which form protein-protein interactions (PPIs) with Ras and catalyze the exchange of GDP by GTP. This is the rate-limiting step in Ras activation. However, Ras surfaces lack any evident suitable pockets where a molecule might bind tightly, rendering Ras proteins still 'undruggable' for over 30 years. Among the alternative approaches is the design of inhibitors that target the Ras-SOS PPI interface, a strategy that is gaining increasing recognition for treating Ras mutant cancers. Herein we focus on data that has accumulated over the past few years pertaining to the design of small-molecule modulators or peptide mimetics aimed at the interface of the Ras-SOS PPI. We emphasize, however, that even if such Ras-SOS therapeutics are potent, drug resistance may emerge. To counteract this development, we propose "pathway drug cocktails", that is, drug combinations aimed at parallel (or compensatory) pathways. A repertoire of classified cancer, cell/tissue, and pathway/protein combinations would be beneficial toward this goal. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. Theoretical model of the SOS effect

    Energy Technology Data Exchange (ETDEWEB)

    Darznek, S A; Mesyats, G A; Rukin, S N; Tsiranov, S N [Russian Academy of Sciences, Ural Division, Ekaterinburg (Russian Federation). Institute of Electrophysics

    1997-12-31

    Physical principles underlying the operation of semiconductor opening switches (SOS) are highlighted. The SOS effect occurs at a current density of up to 60 kA/cm{sup 2} in silicon p{sup +}-p-n-n{sup +} structures filled with residual electron-hole plasma. Using a theoretical model developed for plasma dynamic calculations, the mechanism by which current passes through the structure at the stage of high conduction and the processes that take place at the stage of current interruption were analyzed. The dynamics of the processes taking place in the structure was calculated with allowance for both diffusive and drift mechanisms of carrier transport. In addition, two recombination types, viz. recombination via impurities and impact Auger recombination, were included in the model. The effect of the structure on the pumping-circuit current and voltage was also taken into account. The real distribution of the doped impurity in the structure and the avalanche mechanism of carrier multiplication were considered. The results of calculations of a typical SOS are presented. The dynamics of the electron-hole plasma is analyzed. It is shown that the SOS effect represents a qualitatively new mechanism of current interruption in semiconductor structures. (author). 4 figs., 7 refs.

  6. SoS contract verification using statistical model checking

    Directory of Open Access Journals (Sweden)

    Alessandro Mignogna

    2013-11-01

    Full Text Available Exhaustive formal verification for systems of systems (SoS is impractical and cannot be applied on a large scale. In this paper we propose to use statistical model checking for efficient verification of SoS. We address three relevant aspects for systems of systems: 1 the model of the SoS, which includes stochastic aspects; 2 the formalization of the SoS requirements in the form of contracts; 3 the tool-chain to support statistical model checking for SoS. We adapt the SMC technique for application to heterogeneous SoS. We extend the UPDM/SysML specification language to express the SoS requirements that the implemented strategies over the SoS must satisfy. The requirements are specified with a new contract language specifically designed for SoS, targeting a high-level English- pattern language, but relying on an accurate semantics given by the standard temporal logics. The contracts are verified against the UPDM/SysML specification using the Statistical Model Checker (SMC PLASMA combined with the simulation engine DESYRE, which integrates heterogeneous behavioral models through the functional mock-up interface (FMI standard. The tool-chain allows computing an estimation of the satisfiability of the contracts by the SoS. The results help the system architect to trade-off different solutions to guide the evolution of the SoS.

  7. Prevalência de isquemia induzida por estresse mental Prevalence of induced ischemia by mental distress

    Directory of Open Access Journals (Sweden)

    Gustavo Borges Barbirato

    2010-03-01

    Full Text Available FUNDAMENTOS: A cintilografia miocárdica com estresse mental parece induzir isquemia através de uma fisiopatologia particular quando comparada com a cintilografia, utilizando o estresse físico ou farmacológico. OBJETIVO: Avaliar a prevalência de isquemia miocárdica induzida por estresse mental, em pacientes com dor torácica e cintilografia com estresse convencional normal, utilizando 99mTc-Sestamibi. MÉTODOS: 22 PAcientes foram admitidos com dor torácica na emergência, ou foram encaminhados ambulatorialmente ao serviço de medicina nuclear da nossa instituição, onde realizaram cintilografia miocárdica de estresse e repouso sem alterações isquêmicas. Então, foram convidados a realizar uma fase adicional com indução de estresse mental através do conflito de cores (Stroop Color Test com o objetivo de detectar isquemia miocárdica. Dois cardiologistas e médicos nucleares realizaram a análise cega dos dados perfusionais e consequente quantificação através do SDS (Summed Diference Score, pontuando os segmentos com alteração perfusional após o estresse mental e comparando com a imagem de repouso. A presença de isquemia miocárdica foi considerada com SDS > 3. RESULTADOS: A prevalência de isquemia miocárdica induzida por estresse mental foi de 40% (9 pacientes positivos. Nos 22 pacientes estudados não houve diferença estatística quanto ao número de fatores de risco, alterações hemodinâmicas induzidas pelo estresse mental, uso de medicações, sintomas apresentados, presença ou ausência de doença coronariana e variações da fração de ejeção e volume sistólico final do Gated SPECT. CONCLUSÃO: EM Uma amostra selecionada de pacientes com dor torácica e cintilografia miocárdica convencional normal, a pesquisa de isquemia miocárdica induzida pelo estresse mental através de cintilografia pode ser positiva em até 40% dos casos.BACKGROUND: The myocardial radionuclide imaging with mental distress seems to induce

  8. SOS Móvel: sistema para auxiliar pessoas na solicitação de socorro

    Directory of Open Access Journals (Sweden)

    Suellem Stephanne Fernandes Queiroz

    2016-11-01

    Full Text Available Devido aos índices elevados de acidentes, incidentes e ocorrências, qualquer um está vulnerável e pode necessitar de informações sobre centros de saúde mais próximos para o socorro. Na busca de reduzir o alto índice de ocorrências fatais em acidentes e incidentes quaisquer, medidas tecnológicas passaram a ser empregadas para contatar rapidamente o socorro. O SOS móvel é um sistema para auxiliar pessoas na solicitação de socorro. O sistema faz reconhecimento de atividades, utiliza sensores móveis com sensibilidade ao contexto e um agente inteligente para detecção de situações de risco e disparo de solicitações de socorro automáticas. O sistema é composto por uma parte web e uma móvel. O aplicativo móvel é utilizado ativamente pelo usuário, enquanto que a interface web é utilizada pelos familiares ou gestores da saúde que podem monitorar o usuário e visualizar sua localização e alertas. Os experimentos com o SOS Móvel foram realizados em um ambiente de simulações e permitiram concluir que esse apresentou desempenho satisfatório ao detectar riscos e fazer o envio de solicitações de socorro automaticamente.

  9. The Salt Overly Sensitive (SOS) pathway: established and emerging roles.

    Science.gov (United States)

    Ji, Hongtao; Pardo, José M; Batelli, Giorgia; Van Oosten, Michael J; Bressan, Ray A; Li, Xia

    2013-03-01

    Soil salinity is a growing problem around the world with special relevance in farmlands. The ability to sense and respond to environmental stimuli is among the most fundamental processes that enable plants to survive. At the cellular level, the Salt Overly Sensitive (SOS) signaling pathway that comprises SOS3, SOS2, and SOS1 has been proposed to mediate cellular signaling under salt stress, to maintain ion homeostasis. Less well known is how cellularly heterogenous organs couple the salt signals to homeostasis maintenance of different types of cells and to appropriate growth of the entire organ and plant. Recent evidence strongly indicates that different regulatory mechanisms are adopted by roots and shoots in response to salt stress. Several reports have stated that, in roots, the SOS proteins may have novel roles in addition to their functions in sodium homeostasis. SOS3 plays a critical role in plastic development of lateral roots through modulation of auxin gradients and maxima in roots under mild salt conditions. The SOS proteins also play a role in the dynamics of cytoskeleton under stress. These results imply a high complexity of the regulatory networks involved in plant response to salinity. This review focuses on the emerging complexity of the SOS signaling and SOS protein functions, and highlights recent understanding on how the SOS proteins contribute to different responses to salt stress besides ion homeostasis.

  10. Avaliação da fosfatase alcalina óssea e hepática em gatos com tirotoxicose induzida

    Directory of Open Access Journals (Sweden)

    Fabiano Séllos Costa

    2009-10-01

    Full Text Available A tirotoxicose é caracterizada pelas excessivas concentrações séricas dos hormônios tiroidianos, podendo desencadear graves alterações no metabolismo ósseo, sendo a elevação da fosfatase alcalina total uma alteração laboratorial freqüentemente observada no hipertiroidismo felino. O aumento global dos níveis séricos de fosfatase alcalina pode ser decorrente de diferentes isoenzimas e, no caso do hipertiroidismo em humanos, as isoenzimas de origem óssea e hepática apresentam-se comumente elevadas. A partir da avaliação da bioquímica sérica de oito gatos com tirotoxicose induzida e elevação da fosfatase alcalina associada, o presente trabalho demonstra um aumento significativamente maior dos níveis séricos da fosfatase alcalina de origem óssea quando comparado com a isoenzima de origem hepática. Conclui-se que as alterações no metabolismo ósseo foram as principais responsáveis pelo aumento da fosfatase alcalina nos gatos com tirotoxicose induzida.

  11. Lesao pulmonar induzida pela ventilacao em recem-nascidos prematuros

    Directory of Open Access Journals (Sweden)

    Clarissa Gutierrez Carvalho

    2013-12-01

    Full Text Available A necessidade de intubação e do uso de ventilação mecânica na prematuridade está relacionada à chamada lesão pulmonar induzida pela ventilação e à consequente displasia broncopulmonar. Busca-se a melhor compreensão dos mecanismos de lesão envolvendo resposta inflamatória mediada pelas citocinas para o desenvolvimento de novas estratégias protetoras. Pesquisou-se na base de dados PubMed, incluindo artigos relevantes, os unitermos "ventilator induced lung injury preterm", "continuous positive airway pressure", "preterm" e "bronchopulmonary dysplasia". Dados e informações significativas foram compilados em tópicos, com o objetivo de formar uma visão crítica e plena acerca da lesão induzida pela ventilação e de suas consequências ao prematuro. Foi revisado o papel das citocinas pró-inflamatórias como mediadores da lesão, especialmente interleucinas 6 e 8, e fator de necrose tumoral alfa. Foram apresentadas evidências em estudos com animais e também em humanos, mostrando que breves períodos de ventilação mecânica são suficientes para a liberação dessas interleucinas inflamatórias. Também foram revisadas outras formas de ventilação mecânica e de ventilação não invasiva, como alternativas protetoras aos modos convencionais. Concluiu-se que o uso de ventilação não invasiva, a intubação com administração precoce de surfactante e a extubação rápida para CPAP nasal, além de estratégias que regulam o volume corrente evitando o volutrauma (como a ventilação com volume garantido, são medidas protetoras da lesão pulmonar induzida pela ventilação mecânica no prematuro.

  12. Advances/applications of MAGIC and SOS

    Science.gov (United States)

    Warren, Gary; Ludeking, Larry; Nguyen, Khanh; Smithe, David; Goplen, Bruce

    1993-12-01

    MAGIC and SOS have been applied to investigate a variety of accelerator-related devices. Examples include high brightness electron guns, beam-RF interactions in klystrons, cold-test modes in an RFQ and in RF sources, and a high-quality, flexible, electron gun with operating modes appropriate for gyrotrons, peniotrons, and other RF sources. Algorithmic improvements for PIC have been developed and added to MAGIC and SOS to facilitate these modeling efforts. Two new field algorithms allow improved control of computational numerical noise and selective control of harmonic modes in RF cavities. An axial filter in SOS accelerates simulations in cylindrical coordinates. The recent addition of an export/import feature now allows long devices to be modeled in sections. Interfaces have been added to receive electromagnetic field information from the Poisson group of codes and from EGUN and to send beam information to PARMELA for subsequent tracing of bunches through beam optics. Post-processors compute and display beam properties including geometric, normalized, and slice emittances, and phase-space parameters, and video. VMS, UNIX, and DOS versions are supported, with migration underway toward windows environments.

  13. Distúrbios causados pelo frio e pelo calor durante corridas de longa distância

    Directory of Open Access Journals (Sweden)

    1999-06-01

    Full Text Available Muitos corredores amadores e de elite participam todos os anos de corridas de longa distância. Quando estes eventos ocorrem em temperaturas altas ou baixas, aumenta o risco de distúrbios térmicos. Contudo, a hipertermia induzida pelo exercício, a hipotermia, a desidratação e outros problemas relacionados podem ser minimizados através de esclarecimentos e um preparo antes do evento. Este documento fornece recomendações para o diretor médico e outras autoridades da organização das corridas nas seguintes áreas: planejamento, profissionais envolvidos na organização, instalações, suprimentos, equipamento e comunicação; fornecimento de esclarecimentos aos participantes; avaliação do estresse térmico; fornecimento de líquidos; e prevenção de questões legais em potencial. Este posicionamento oficial também descreve as condições predisponentes, as formas de diagnóstico e o tratamento dos quatro distúrbios ambientais mais comuns: exaustão pelo calor, colapso pelo calor, hipotermia e congelamento de extremidades. Os objetivos deste documento são: 1 Educar os organizadores e os participantes de corridas de longa distância a respeito das formas mais comuns de distúrbios térmicos incluindo as condições predisponentes, sinais de alerta, suscetibilidade e a redução de sua incidência; 2 Alertar os organizadores sobre as suas responsabilidades civis em potencial no que concerne à segurança do evento e à prevenção de lesões; 3 Recomendar que os organizadores consultem arquivos locais de meteorologia e planejem eventos em horas que provavelmente causem menos estresse térmico de modo a minimizar os efeitos deletérios sobre os participantes; 4 Estimular os organizadores a alertar os participantes sobre o estresse térmico no dia da corrida e as suas implicações no que tange aos distúrbios causados pelo frio e pelo calor; 5 Informar os organizadores sobre as ações preventivas que podem reduzir a incidência dos

  14. Ação do tacrolimus na pancreatite aguda experimental induzida pela arginina

    Directory of Open Access Journals (Sweden)

    Marlus Moreira

    Full Text Available OBJETIVO: verificar se o tacrolimus administrado em ratos, em vigência de pancreatite induzida pela L-Arginina, interfere nos níveis séricos da amilase e glicose e no padrão histológico do parênquima pancreático. MÉTODOS: quarenta ratos Wistar foram distribuídos em quatro grupos com 10 ratos cada. Grupo controle (C, grupo tacrolimus (T, grupo pancreatite (P e grupo pancreatite-tacrolimus (PT. Foram avaliados os níveis séricos de amilase, glicose e tacrolimus e feitas avaliações histológicas do pâncreas, A indução de pancreatite foi feita pela inoculação de L-Arginina na dose de 500mg/100g de peso corporal por via intraperitoneal e o tratamento com tacrolimus na dose de 1ìg/kg por via subcutânea durante quatro dias. RESULTADOS: a amilasemia estava mais elevada (p=0,0000 nos grupos PT, T e P do que no grupo controle. A média do grupo PT foi maior (p=0,0009 que a do grupo T, mas não diferiu (p=0,6802 da média do grupo P. Entre os grupos P e T não houve diferença (p=0,2568. Não houve diferença nas médias de glicemia entre os grupos (p=0,4920 e os níveis séricos de tacrolimus foram similares nos grupos PT e T (p=0,7112. Não ocorreram alterações histológicas nos grupos T e C e não ocorreu hemorragia no pâncreas dos ratos dos grupos P e PT. No grupo P, em 30% não se observou edema, em 20% observou-se a forma leve e em 50%, a moderada; quanto à infiltração inflamatória, em 80% moderada e em 20% não ocorreu, e a atrofia do parênquima foi de 60% moderada e 40% acentuada. No grupo PT, houve ocorrência de edema, infiltração inflamatória e atrofia do pâncreas em todos os ratos. CONCLUSÃO: o tratamento pelo tacrolimus induziu aumento nos níveis séricos de amilase em ratos normais, não alterou a glicemia nem o padrão histológico do parênquima pancreático. Na vigência de pancreatite induzida pela L-Arginina o tacrolimus induziu edema, infiltração inflamatória e atrofia com maior gravidade no par

  15. Mapping Modular SOS to Rewriting Logic

    DEFF Research Database (Denmark)

    Braga, Christiano de Oliveira; Haeusler, Edward Hermann; Meseguer, José

    2003-01-01

    and verification of MSOS specifications, we have defined a mapping, named , from MSOS to rewriting logic (RWL), a logic which has been proposed as a logical and semantic framework. We have proven the correctness of and implemented it as a prototype, the MSOS-SL Interpreter, in the Maude system, a high......Modular SOS (MSOS) is a framework created to improve the modularity of structural operational semantics specifications, a formalism frequently used in the fields of programming languages semantics and process algebras. With the objective of defining formal tools to support the execution...

  16. Evelin Ilves avas SOS Lasteküla

    Index Scriptorium Estoniae

    2010-01-01

    SOS Lasteküla patroon proua Evelin Ilves avas 1. juunil 2010 Põltsamaal Eesti teise SOS Lasteküla. Presidendi abikaasa tõi kingiks õunapuuistikuid ja lasteraamatuid. Ilmunud ka: Eesti Päevaleht 2. juuni 2010, lk. 4

  17. Terapia por contensão induzida: revisão de ensaios clínicos

    Directory of Open Access Journals (Sweden)

    Lidiane de Araújo Silva

    Full Text Available INTRODUÇÃO: A Terapia por Contensão Induzida (TCI é uma terapêutica que visa à recuperação da função do membro superior (MS parético de pacientes com sequelas de um acidente vascular cerebral por meio de treinamento intensivo, uso de uma restrição no MS não-parético durante 90% do dia e pelo pacote de transferência. OBJETIVO: O objetivo deste trabalho é realizar uma revisão entre os estudos que compararem a TCI com outras terapêuticas. METODOLOGIA: Foi realizada uma pesquisa nas bases de dados MEDLINE, SciELOo e ISI Web of Science com o descritor "constraint induced movement therapy" durante o mês de junho de 2008. RESULTADOS: Foram encontrados seis artigos onde a TCI foi comparada com os métodos Bobath e Kabat. Discussão: Dentre os artigos encontrados, percebemos que há uma variação entre as amostras de cada estudo, assim como o método de avaliação entre as terapêuticas. CONCLUSÃO: A TCI mostrou-se superior em relação às outras terapêuticas.

  18. Ras activation by SOS: Allosteric regulation by altered fluctuation dynamics

    Science.gov (United States)

    Iversen, Lars; Tu, Hsiung-Lin; Lin, Wan-Chen; Christensen, Sune M.; Abel, Steven M.; Iwig, Jeff; Wu, Hung-Jen; Gureasko, Jodi; Rhodes, Christopher; Petit, Rebecca S.; Hansen, Scott D.; Thill, Peter; Yu, Cheng-Han; Stamou, Dimitrios; Chakraborty, Arup K.; Kuriyan, John; Groves, Jay T.

    2014-01-01

    Activation of the small guanosine triphosphatase H-Ras by the exchange factor Son of Sevenless (SOS) is an important hub for signal transduction. Multiple layers of regulation, through protein and membrane interactions, govern activity of SOS. We characterized the specific activity of individual SOS molecules catalyzing nucleotide exchange in H-Ras. Single-molecule kinetic traces revealed that SOS samples a broad distribution of turnover rates through stochastic fluctuations between distinct, long-lived (more than 100 seconds), functional states. The expected allosteric activation of SOS by Ras–guanosine triphosphate (GTP) was conspicuously absent in the mean rate. However, fluctuations into highly active states were modulated by Ras-GTP. This reveals a mechanism in which functional output may be determined by the dynamical spectrum of rates sampled by a small number of enzymes, rather than the ensemble average. PMID:24994643

  19. The investigation of SOS-response of Escherichia coli after γ-irradiation by means of SOS-chromotest

    International Nuclear Information System (INIS)

    Kozubek, S.; Ogievetskaya, M.M.; Krasavin, E.A.; Drasil, V.; Soska, J.

    1988-01-01

    The kinetics of the E.coli PQ37 SOS-system induction by γ-radiation has been studied by the SOS-chromotest technique. The experimental data are consistent with the following hypotheses. The production of DNA damages inducing the SOS-system is 0,021 Gy -1 per genome. The SOS-system is switched off approximately 200 min after γ-irradiation. The spontaneous triggering of the SOS-system is induced in the exponentially growing cells. The probability of its induction is independent of time up to 180 min of incubation. The synthesis of constitutive alkaline phosphatase proceeds for some time in the cells that suffered lethal damages from γ-irradiation. A correction has been proposed for the calculation of the induction factor. 5 refs.; 11 figs

  20. Influencia do tempo de exposicao a obesidade induzida por dieta hiperlipidica sobre os colagenos tipo I e III miocardico

    Directory of Open Access Journals (Sweden)

    Danielle Cristina Tomaz da Silva

    2014-03-01

    Full Text Available FUNDAMENTO: A obesidade é um fator de risco para muitas complicações médicas; a pesquisa médica demonstrou que as alterações hemodinâmicas, morfológicas e funcionais estão correlacionadas com a duração e gravidade da obesidade. OBJETIVO: O presente estudo determinou a influência do tempo de exposição à obesidade induzida por dieta com alto teor de gordura no colágenos tipo I e III miocárdico. MÉTODOS: Ratos machos com trinta dias de idade, da raça Wistar, foram distribuídos aleatoriamente em dois grupos: um grupo de controle (C alimentado com ração padrão e um grupo de ratos obesos (Ob alternadamente alimentados com uma de quatro dietas palatáveis ricas em gordura. Cada dieta foi mudada diariamente, e os ratos foram mantidos em suas respectivas dietas por 15 (C15 e Ob15 e 30 (C30 e Ob30 semanas consecutivas. A obesidade foi determinada pelo índice de adiposidade. RESULTADOS: O grupo Ob15 foi similar ao grupo C15 em relação à expressão de colágeno miocárdico tipo I; contudo, a expressão no grupo Ob30 foi menor do que no grupo C30. O tempo de exposição à obesidade foi associado com uma redução de colágeno do tipo I no grupo Ob30, quando comparado com o Ob15. A obesidade não afetou a expressão do colágeno tipo III. CONCLUSÃO: Este estudo mostrou que o tempo de exposição à obesidade por 30 semanas induzida por uma dieta rica em gordura insaturada causou uma redução na expressão do colágeno miocárdico tipo I em ratos obesos. No entanto, nenhum efeito foi observado em relação à expressão do colágeno miocárdico tipo III

  1. Adaptive symbiotic organisms search (SOS algorithm for structural design optimization

    Directory of Open Access Journals (Sweden)

    Ghanshyam G. Tejani

    2016-07-01

    Full Text Available The symbiotic organisms search (SOS algorithm is an effective metaheuristic developed in 2014, which mimics the symbiotic relationship among the living beings, such as mutualism, commensalism, and parasitism, to survive in the ecosystem. In this study, three modified versions of the SOS algorithm are proposed by introducing adaptive benefit factors in the basic SOS algorithm to improve its efficiency. The basic SOS algorithm only considers benefit factors, whereas the proposed variants of the SOS algorithm, consider effective combinations of adaptive benefit factors and benefit factors to study their competence to lay down a good balance between exploration and exploitation of the search space. The proposed algorithms are tested to suit its applications to the engineering structures subjected to dynamic excitation, which may lead to undesirable vibrations. Structure optimization problems become more challenging if the shape and size variables are taken into account along with the frequency. To check the feasibility and effectiveness of the proposed algorithms, six different planar and space trusses are subjected to experimental analysis. The results obtained using the proposed methods are compared with those obtained using other optimization methods well established in the literature. The results reveal that the adaptive SOS algorithm is more reliable and efficient than the basic SOS algorithm and other state-of-the-art algorithms.

  2. An orthosteric inhibitor of the RAS-SOS interaction.

    Science.gov (United States)

    Nickerson, Seth; Joy, Stephen T; Arora, Paramjit S; Bar-Sagi, Dafna

    2013-01-01

    Rat sarcoma (RAS) proteins are signaling nodes that transduce extracellular cues into precise alterations in cellular physiology by engaging effector pathways. RAS signaling thus regulates diverse cell processes including proliferation, migration, differentiation, and survival. Owing to this central role in governing mitogenic signals, RAS pathway components are often dysregulated in human diseases. Targeted therapy of RAS pathways has generally not been successful, largely because of the robust biochemistry of the targets and their multifaceted network of molecular regulators. The rate-limiting step of RAS activation is Son of Sevenless (SOS)-mediated nucleotide exchange involving a single evolutionarily conserved catalytic helix from SOS. Structure function data of this mechanism provided a strong platform to design an SOS-derived, helically constrained peptide mimic as an inhibitor of the RAS-SOS interaction. In this chapter, we review RAS-SOS signaling dynamics and present evidence supporting the novel paradigm of inhibiting their interaction as a therapeutic strategy. We then describe a method of generating helically constrained peptide mimics of protein surfaces, which we have employed to inhibit the RAS-SOS active site interaction. The biochemical and functional properties of this SOS mimic support the premise that inhibition of RAS-nucleotide exchange can effectively block RAS activation and downstream signaling. © 2013 Elsevier Inc. All rights reserved.

  3. Reconstitution in yeast of the Arabidopsis SOS signaling pathway for Na+ homeostasis

    OpenAIRE

    Quintero, Francisco J.; Ohta, Masaru; Shi, Huazhong; Zhu, Jian-Kang; Pardo, José M.

    2002-01-01

    The Arabidopsis thaliana SOS1 protein is a putative Na H antiporter that functions in Na extrusion and is essential for the NaCl tolerance of plants. sos1 mutant plants share phenotypic similarities with mutants lacking the protein kinase SOS2 and the Ca2 sensor SOS3. To investigate whether the three SOS proteins function in the same response pathway, we have reconstituted the SOS system in yeast cells. Expression of SOS1 improved the Na tolerance of yeast mutants la...

  4. SOS response and its regulation on the fluoroquinolone resistance.

    Science.gov (United States)

    Qin, Ting-Ting; Kang, Hai-Quan; Ma, Ping; Li, Peng-Peng; Huang, Lin-Yan; Gu, Bing

    2015-12-01

    Bacteria can survive fluoroquinolone antibiotics (FQs) treatment by becoming resistant through a genetic change-mutation or gene acquisition. The SOS response is widespread among bacteria and exhibits considerable variation in its composition and regulation, which is repressed by LexA protein and derepressed by RecA protein. Here, we take a comprehensive review of the SOS gene network and its regulation on the fluoroquinolone resistance. As a unique survival mechanism, SOS may be an important factor influencing the outcome of antibiotic therapy in vivo.

  5. Sensor Data from the NERACOOS SOS Server, 2000-present

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Northeastern Regional Association of Coastal Ocean Observing Systems (NERACOOS) Sensor Observation Service (SOS) The OCEANS IE -- formally approved as an OGC...

  6. SOS - Der kaldes på Smartere Offentlig Styring

    DEFF Research Database (Denmark)

    Hjortdal, Henrik

    2017-01-01

    Smartere Offentlig Styring eller SOS. Det var temaet da over hundrede kommunale direktører drøftede offentlig ledelse med en lang række toneangivende danske forskere.......Smartere Offentlig Styring eller SOS. Det var temaet da over hundrede kommunale direktører drøftede offentlig ledelse med en lang række toneangivende danske forskere....

  7. SOS hotline for women victims of discrimination at the workplace

    OpenAIRE

    Dobrosavljević-Grujić Ljiljana S.

    2004-01-01

    SOS hotline for women victims of discrimination at the workplace offers free legal assistance to women, whenever their labor rights are endangered. If the dispute cannot be resolved peacefully by mediation between employer and employees, female lawyer skilled for the labor law starts up judicial proceedings. Some characteristic cases of discrimination of women from the practice of SOS telephone, such as dismissal from the work, physical violence and sexual blackmail, are presented in the paper.

  8. SOS hotline for women victims of discrimination at the workplace

    Directory of Open Access Journals (Sweden)

    Dobrosavljević-Grujić Ljiljana S.

    2004-01-01

    Full Text Available SOS hotline for women victims of discrimination at the workplace offers free legal assistance to women, whenever their labor rights are endangered. If the dispute cannot be resolved peacefully by mediation between employer and employees, female lawyer skilled for the labor law starts up judicial proceedings. Some characteristic cases of discrimination of women from the practice of SOS telephone, such as dismissal from the work, physical violence and sexual blackmail, are presented in the paper.

  9. Ribonuclease E modulation of the bacterial SOS response.

    Directory of Open Access Journals (Sweden)

    Robert Manasherob

    Full Text Available Plants, animals, bacteria, and Archaea all have evolved mechanisms to cope with environmental or cellular stress. Bacterial cells respond to the stress of DNA damage by activation of the SOS response, the canonical RecA/LexA-dependent signal transduction pathway that transcriptionally derepresses a multiplicity of genes-leading to transient arrest of cell division and initiation of DNA repair. Here we report the previously unsuspected role of E. coli endoribonuclease RNase E in regulation of the SOS response. We show that RNase E deletion or inactivation of temperature-sensitive RNase E protein precludes normal initiation of SOS. The ability of RNase E to regulate SOS is dynamic, as down regulation of RNase E following DNA damage by mitomycin C resulted in SOS termination and restoration of RNase E function leads to resumption of a previously aborted response. Overexpression of the RraA protein, which binds to the C-terminal region of RNase E and modulates the actions of degradosomes, recapitulated the effects of RNase E deficiency. Possible mechanisms for RNase E effects on SOS are discussed.

  10. SOS, the formidable strategy of bacteria against aggressions.

    Science.gov (United States)

    Baharoglu, Zeynep; Mazel, Didier

    2014-11-01

    The presence of an abnormal amount of single-stranded DNA in the bacterial cell constitutes a genotoxic alarm signal that induces the SOS response, a broad regulatory network found in most bacterial species to address DNA damage. The aim of this review was to point out that beyond being a repair process, SOS induction leads to a very strong but transient response to genotoxic stress, during which bacteria can rearrange and mutate their genome, induce several phenotypic changes through differential regulation of genes, and sometimes acquire characteristics that potentiate bacterial survival and adaptation to changing environments. We review here the causes and consequences of SOS induction, but also how this response can be modulated under various circumstances and how it is connected to the network of other important stress responses. In the first section, we review articles describing the induction of the SOS response at the molecular level. The second section discusses consequences of this induction in terms of DNA repair, changes in the genome and gene expression, and sharing of genomic information, with their effects on the bacteria's life and evolution. The third section is about the fine tuning of this response to fit with the bacteria's 'needs'. Finally, we discuss recent findings linking the SOS response to other stress responses. Under these perspectives, SOS can be perceived as a powerful bacterial strategy against aggressions. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

  11. Ribonuclease E modulation of the bacterial SOS response.

    Science.gov (United States)

    Manasherob, Robert; Miller, Christine; Kim, Kwang-sun; Cohen, Stanley N

    2012-01-01

    Plants, animals, bacteria, and Archaea all have evolved mechanisms to cope with environmental or cellular stress. Bacterial cells respond to the stress of DNA damage by activation of the SOS response, the canonical RecA/LexA-dependent signal transduction pathway that transcriptionally derepresses a multiplicity of genes-leading to transient arrest of cell division and initiation of DNA repair. Here we report the previously unsuspected role of E. coli endoribonuclease RNase E in regulation of the SOS response. We show that RNase E deletion or inactivation of temperature-sensitive RNase E protein precludes normal initiation of SOS. The ability of RNase E to regulate SOS is dynamic, as down regulation of RNase E following DNA damage by mitomycin C resulted in SOS termination and restoration of RNase E function leads to resumption of a previously aborted response. Overexpression of the RraA protein, which binds to the C-terminal region of RNase E and modulates the actions of degradosomes, recapitulated the effects of RNase E deficiency. Possible mechanisms for RNase E effects on SOS are discussed.

  12. Sinusoidal obstruction syndrome (SOS) related to chemotherapy for colorectal liver metastases: factors predictive of severe SOS lesions and protective effect of bevacizumab.

    Science.gov (United States)

    Hubert, Catherine; Sempoux, Christine; Humblet, Yves; van den Eynde, Marc; Zech, Francis; Leclercq, Isabelle; Gigot, Jean-François

    2013-11-01

    The most frequent presentation of chemotherapy-related toxicity in colorectal liver metastases (CRLM) is sinusoidal obstruction syndrome (SOS). The purpose of the present study was to identify preoperative factors predictive of SOS and to establish associations between type of chemotherapy and severity of SOS. A retrospective study was carried out in a tertiary academic referral hospital. Patients suffering from CRLM who had undergone resection of at least one liver segment were included. Grading of SOS on the non-tumoral liver parenchyma was accomplished according to the Rubbia-Brandt criteria. A total of 151 patients were enrolled and divided into four groups according to the severity of SOS (grades 0-3). Multivariate analysis identified oxaliplatin and 5-fluorouracil as chemotherapeutic agents responsible for severe SOS lesions (P SOS lesions (P = 0.005). Univariate analysis identified the score on the aspartate aminotransferase : platelets ratio index (APRI) as the most significant biological factor predictive of severe SOS lesions. Splenomegaly is also significantly associated with the occurrence of severe SOS lesions. The APRI score and splenomegaly are effective as factors predictive of SOS. Bevacizumab has a protective effect against SOS. © 2013 International Hepato-Pancreato-Biliary Association.

  13. Quinolone Resistance Reversion by Targeting the SOS Response.

    Science.gov (United States)

    Recacha, E; Machuca, J; Díaz de Alba, P; Ramos-Güelfo, M; Docobo-Pérez, F; Rodriguez-Beltrán, J; Blázquez, J; Pascual, A; Rodríguez-Martínez, J M

    2017-10-10

    Suppression of the SOS response has been postulated as a therapeutic strategy for potentiating antimicrobial agents. We aimed to evaluate the impact of its suppression on reversing resistance using a model of isogenic strains of Escherichia coli representing multiple levels of quinolone resistance. E. coli mutants exhibiting a spectrum of SOS activity were constructed from isogenic strains carrying quinolone resistance mechanisms with susceptible and resistant phenotypes. Changes in susceptibility were evaluated by static (MICs) and dynamic (killing curves or flow cytometry) methodologies. A peritoneal sepsis murine model was used to evaluate in vivo impact. Suppression of the SOS response was capable of resensitizing mutant strains with genes encoding three or four different resistance mechanisms (up to 15-fold reductions in MICs). Killing curve assays showed a clear disadvantage for survival (Δlog 10 CFU per milliliter [CFU/ml] of 8 log units after 24 h), and the in vivo efficacy of ciprofloxacin was significantly enhanced (Δlog 10 CFU/g of 1.76 log units) in resistant strains with a suppressed SOS response. This effect was evident even after short periods (60 min) of exposure. Suppression of the SOS response reverses antimicrobial resistance across a range of E. coli phenotypes from reduced susceptibility to highly resistant, playing a significant role in increasing the in vivo efficacy. IMPORTANCE The rapid rise of antibiotic resistance in bacterial pathogens is now considered a major global health crisis. New strategies are needed to block the development of resistance and to extend the life of antibiotics. The SOS response is a promising target for developing therapeutics to reduce the acquisition of antibiotic resistance and enhance the bactericidal activity of antimicrobial agents such as quinolones. Significant questions remain regarding its impact as a strategy for the reversion or resensitization of antibiotic-resistant bacteria. To address this

  14. Quinolone Resistance Reversion by Targeting the SOS Response

    Directory of Open Access Journals (Sweden)

    E. Recacha

    2017-10-01

    Full Text Available Suppression of the SOS response has been postulated as a therapeutic strategy for potentiating antimicrobial agents. We aimed to evaluate the impact of its suppression on reversing resistance using a model of isogenic strains of Escherichia coli representing multiple levels of quinolone resistance. E. coli mutants exhibiting a spectrum of SOS activity were constructed from isogenic strains carrying quinolone resistance mechanisms with susceptible and resistant phenotypes. Changes in susceptibility were evaluated by static (MICs and dynamic (killing curves or flow cytometry methodologies. A peritoneal sepsis murine model was used to evaluate in vivo impact. Suppression of the SOS response was capable of resensitizing mutant strains with genes encoding three or four different resistance mechanisms (up to 15-fold reductions in MICs. Killing curve assays showed a clear disadvantage for survival (Δlog10 CFU per milliliter [CFU/ml] of 8 log units after 24 h, and the in vivo efficacy of ciprofloxacin was significantly enhanced (Δlog10 CFU/g of 1.76 log units in resistant strains with a suppressed SOS response. This effect was evident even after short periods (60 min of exposure. Suppression of the SOS response reverses antimicrobial resistance across a range of E. coli phenotypes from reduced susceptibility to highly resistant, playing a significant role in increasing the in vivo efficacy.

  15. Consequences of SOS1 deficiency: Intracellular physiology and transcription

    KAUST Repository

    Ha, OhDong

    2010-06-01

    As much as there is known about the function of the sodium/proton antiporter SOS1 in plants, recent studies point towards a more general role for this protein. The crucial involvement in salt stress protection is clearly one of its functions –confined to the N-terminus, but the modular structure of the protein includes a segment with several domains that are functionally not studied but comprise more than half of the protein’s length. Additional functions of the protein appear to be an influence on vesicle trafficking, vacuolar pH and general ion homeostasis during salt stress. Eliminating SOS1 leads to the expression of genes that are not strictly salinity stress related. Functions that are regulated in sos1 mutants included pathogen responses, and effects on circadian rhythm.

  16. NOAA NDBC SOS, 2008-present, sea_floor_depth_below_sea_surface

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA NDBC SOS server is part of the IOOS DIF SOS Project. The stations in this dataset have sea_floor_depth_below_sea_surface data. Because of the nature of SOS...

  17. Activation of the plasma membrane Na/H antiporter salt-overly-sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain

    KAUST Repository

    Quintero, Francisco J.; Martí nez-Atienza, Juliana; Villalta, Irene; Jiang, Xingyu; Kim, Woeyeon; Ali, Zhair; Fujii, Hiroaki; Mendoza, Imelda; Yun, Daejin; Zhu, Jian-Kang; Pardo, José Manuel

    2011-01-01

    The plasma membrane sodium/proton exchanger Salt-Overly-Sensitive 1 (SOS1) is a critical salt tolerance determinant in plants. The SOS2-SOS3 calcium-dependent protein kinase complex upregulates SOS1 activity, but the mechanistic details of this crucial event remain unresolved. Here we show that SOS1 is maintained in a resting state by a C-terminal auto-inhibitory domain that is the target of SOS2-SOS3. The auto-inhibitory domain interacts intramolecularly with an adjacent domain of SOS1 that is essential for activity. SOS1 is relieved from auto-inhibition upon phosphorylation of the auto-inhibitory domain by SOS2-SOS3. Mutation of the SOS2 phosphorylation and recognition site impeded the activation of SOS1 in vivo and in vitro. Additional amino acid residues critically important for SOS1 activity and regulation were identified in a genetic screen for hypermorphic alleles.

  18. Activation of the plasma membrane Na/H antiporter salt-overly-sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain

    KAUST Repository

    Quintero, Francisco J.

    2011-01-24

    The plasma membrane sodium/proton exchanger Salt-Overly-Sensitive 1 (SOS1) is a critical salt tolerance determinant in plants. The SOS2-SOS3 calcium-dependent protein kinase complex upregulates SOS1 activity, but the mechanistic details of this crucial event remain unresolved. Here we show that SOS1 is maintained in a resting state by a C-terminal auto-inhibitory domain that is the target of SOS2-SOS3. The auto-inhibitory domain interacts intramolecularly with an adjacent domain of SOS1 that is essential for activity. SOS1 is relieved from auto-inhibition upon phosphorylation of the auto-inhibitory domain by SOS2-SOS3. Mutation of the SOS2 phosphorylation and recognition site impeded the activation of SOS1 in vivo and in vitro. Additional amino acid residues critically important for SOS1 activity and regulation were identified in a genetic screen for hypermorphic alleles.

  19. Onderzoek naar de toepasbaarheid van SOS-chromotest

    NARCIS (Netherlands)

    Voogd CE; van der Stel JJ; Verharen HW; van Bruchem MC

    1988-01-01

    Met 35 stoffen werd de mutagene activiteit onderzocht met een SOS-chromotest kit, de Ames-test en de fluctuatietest met Klebsiella pneumoniae. Voorzover het alkylerende stoffen betreft die basenpaar substituties veroorzaken, blijkt er een goede overeenstemming te bestaan met de resultaten van

  20. SOS formats and meta-theory : 20 years after

    NARCIS (Netherlands)

    Mousavi, M.R.; Reniers, M.A.; Groote, J.F.

    2007-01-01

    In 1981 Structural Operational Semantics (SOS) was introduced as a systematic way to define operational semantics of programming languages by a set of rules of a certain shape [G.D. Plotkin, A structural approach to operational semantics, Technical Report DAIMI FN-19, Computer Science Department,

  1. SOS: Observation, Intervention, and Scaffolding towards Successful Online Students

    Science.gov (United States)

    Ainsa, Trisha

    2017-01-01

    Research, reflection, and evaluation of online classes indicated a need for graduated scaffolding for first time students experiencing distance learning. In order to promote student engagement in the online learning process, I designed SOS for beginning online students. Sixty-three online students were offered an opportunity to participate in a…

  2. Refined functional relations for the elliptic SOS model

    Energy Technology Data Exchange (ETDEWEB)

    Galleas, W., E-mail: w.galleas@uu.nl [ARC Centre of Excellence for the Mathematics and Statistics of Complex Systems, University of Melbourne, VIC 3010 (Australia)

    2013-02-21

    In this work we refine the method presented in Galleas (2012) [1] and obtain a novel kind of functional equation determining the partition function of the elliptic SOS model with domain wall boundaries. This functional relation arises from the dynamical Yang-Baxter relation and its solution is given in terms of multiple contour integrals.

  3. Overexpression of SOS genes in ciprofloxacin resistant Escherichia coli mutants.

    Science.gov (United States)

    Pourahmad Jaktaji, Razieh; Pasand, Shirin

    2016-01-15

    Fluoroquinolones are important antibiotics for the treatment of urinary tract infections caused by Escherichia coli. Mutational studies have shown that ciprofloxacin, a member of fluoroquinolones induces SOS response and mutagenesis in pathogenic bacteria which in turn develop antibiotic resistance. However, inhibition of SOS response can increase recombination activity which in turn leads to genetic variation. The aim of this study was to measure 5 SOS genes expressions in nine E. coli mutants with different MICs for ciprofloxacin following exposure to ciprofloxacin. Gene expression was assessed by quantitative real time PCR. Gene alteration assessment was conducted by PCR amplification and DNA sequencing. Results showed that the expression of recA was increased in 5 mutants. This overexpression is not related to gene alteration, and enhances the expression of polB and umuCD genes encoding nonmutagenic and mutagenic polymerases, respectively. The direct relationship between the level of SOS expression and the level of resistance to ciprofloxacin was also indicated. It was concluded that novel therapeutic strategy that inhibits RecA activity would enhance the efficiency of common antibiotics against pathogenic bacteria. Copyright © 2015 Elsevier B.V. All rights reserved.

  4. Physics Teacher SOS: Supporting New Teachers without Pushing an Agenda

    Science.gov (United States)

    Baird, Dean

    2013-01-01

    Few workshops for teachers focus primarily on instruction methods for basic high school physics. In Northern California, Physics Teacher SOS (PTSOS) has gained popularity doing just that. PTSOS workshops are directed toward early-career science teachers, though veterans are welcome too. The program is not influenced by scientific supply companies,…

  5. Refined functional relations for the elliptic SOS model

    International Nuclear Information System (INIS)

    Galleas, W.

    2013-01-01

    In this work we refine the method presented in Galleas (2012) [1] and obtain a novel kind of functional equation determining the partition function of the elliptic SOS model with domain wall boundaries. This functional relation arises from the dynamical Yang–Baxter relation and its solution is given in terms of multiple contour integrals.

  6. Absence of both Sos-1 and Sos-2 in peripheral CD4+ T cells leads to PI3K pathway activation and defects in migration

    Science.gov (United States)

    Guittard, Geoffrey; Kortum, Robert L; Balagopalan, Lakshmi; Çuburu, Nicolas; Nguyen, Phan; Sommers, Connie L; Samelson, Lawrence E

    2015-01-01

    Sos-1 and Sos-2 are ubiquitously expressed Ras-Guanine Exchange Factors involved in Erk-MAP kinase pathway activation. Using mice lacking genes encoding Sos-1 and Sos-2, we evaluated the role of these proteins in peripheral T-cell signaling and function. Our results confirmed that TCR-mediated Erk activation in peripheral CD4+ T cells does not depend on Sos-1 and Sos-2, although IL-2-mediated Erk activation does. Unexpectedly, however, we show an increase in AKT phosphorylation in Sos-1/2dKO CD4+ T cells upon TCR and IL-2 stimulation. Activation of AKT was likely a consequence of increased recruitment of PI3K to Grb2 upon TCR and/or IL-2 stimulation in Sos-1/2dKO CD4+ T cells. The increased activity of the PI3K/AKT pathway led to downregulation of the surface receptor CD62L in Sos-1/2dKO T cells and a subsequent impairment in T-cell migration. PMID:25973715

  7. Absence of both Sos-1 and Sos-2 in peripheral CD4(+) T cells leads to PI3K pathway activation and defects in migration.

    Science.gov (United States)

    Guittard, Geoffrey; Kortum, Robert L; Balagopalan, Lakshmi; Çuburu, Nicolas; Nguyen, Phan; Sommers, Connie L; Samelson, Lawrence E

    2015-08-01

    Sos-1 and Sos-2 are ubiquitously expressed Ras-guanine exchange factors involved in Erk-MAP kinase pathway activation. Using mice lacking genes encoding Sos-1 and Sos-2, we evaluated the role of these proteins in peripheral T-cell signaling and function. Our results confirmed that TCR-mediated Erk activation in peripheral CD4(+) T cells does not depend on Sos-1 and Sos-2, although IL-2-mediated Erk activation does. Unexpectedly, however, we show an increase in AKT phosphorylation in Sos-1/2dKO CD4(+) T cells upon TCR and IL-2 stimulation. Activation of AKT was likely a consequence of increased recruitment of PI3K to Grb2 upon TCR and/or IL-2 stimulation in Sos-1/2dKO CD4(+) T cells. The increased activity of the PI3K/AKT pathway led to downregulation of the surface receptor CD62L in Sos-1/2dKO T cells and a subsequent impairment in T-cell migration. Published 2015. This article is a U.S. Government work and is in the public domain in the USA.

  8. One-way membrane trafficking of SOS in receptor-triggered Ras activation.

    Science.gov (United States)

    Christensen, Sune M; Tu, Hsiung-Lin; Jun, Jesse E; Alvarez, Steven; Triplet, Meredith G; Iwig, Jeffrey S; Yadav, Kamlesh K; Bar-Sagi, Dafna; Roose, Jeroen P; Groves, Jay T

    2016-09-01

    SOS is a key activator of the small GTPase Ras. In cells, SOS-Ras signaling is thought to be initiated predominantly by membrane recruitment of SOS via the adaptor Grb2 and balanced by rapidly reversible Grb2-SOS binding kinetics. However, SOS has multiple protein and lipid interactions that provide linkage to the membrane. In reconstituted-membrane experiments, these Grb2-independent interactions were sufficient to retain human SOS on the membrane for many minutes, during which a single SOS molecule could processively activate thousands of Ras molecules. These observations raised questions concerning how receptors maintain control of SOS in cells and how membrane-recruited SOS is ultimately released. We addressed these questions in quantitative assays of reconstituted SOS-deficient chicken B-cell signaling systems combined with single-molecule measurements in supported membranes. These studies revealed an essentially one-way trafficking process in which membrane-recruited SOS remains trapped on the membrane and continuously activates Ras until being actively removed via endocytosis.

  9. Construct Validity of the Societal Outreach Scale (SOS).

    Science.gov (United States)

    Fike, David S; Denton, Jason; Walk, Matt; Kish, Jennifer; Gorman, Ira

    2018-04-01

    The American Physical Therapy Association (APTA) has been working toward a vision of increasing professional focus on societal-level health. However, performance of social responsibility and related behaviors by physical therapists remain relatively poorly integrated into practice. Promoting a focus on societal outreach is necessary for all health care professionals to impact the health of their communities. The objective was to document the validity of the 14-item Societal Outreach Scale (SOS) for use with practicing physical therapists. This study used a cross-sectional survey. The SOS was transmitted via email to all therapists who were licensed and practicing in 10 states in the United States that were purposefully selected to assure a broad representation. A sample of 2612 usable responses was received. Factor analysis was applied to assess construct validity of the instrument. Of alternate models, a 3-factor model best demonstrated goodness of fit with the sample data according to conventional indices (standardized root mean squared residual = .03, comparative fit index .96, root mean square error of approximation = .06). The 3 factors measured by the SOS were labeled Societal-Level Health Advocacy, Community Engagement/Social Integration, and Political Engagement. Internal consistency reliability was 0.7 for all factors. The 3-factor SOS demonstrated acceptable validity and reliability. Though the sample included a broad representation of physical therapists, this was a single cross-sectional study. Additional confirmatory factor analysis, reliability testing, and word refinement of the tool are warranted. Given the construct validity and reliability of the 3-factor SOS, it is recommended for use as a validated instrument to measure physical therapists' performance of social responsibility and related behaviors.

  10. Mathematical model of the SOS response regulation in wild-type Escherichia coli

    International Nuclear Information System (INIS)

    Aksenov, S.V.

    1997-01-01

    Regulation of the SOS response in Escherichia coli, which is a set of inducible cellular reactions introduced after DNA damage, is due to specific interaction of LexA and RecA proteins. LexA protein is a common repressor of the genes of the SOS system, and RecA protein, once transiently activated by the so-called SOS-inducing signal, promotes LexA protein destruction. We have described the SOS regulation by means of differential equations with regard to LexA and RecA concentrations elsewhere. The 'input' function for model equations is the level of the SOS-inducing signal against time. Here we present a means for calculating the concentration of single-stranded DNA (SOS-inducing signal) as a function of time in wild-type cells after ultraviolet irradiation. With model equations one can simulate kinetic curves of SOS regulatory proteins after DNA damage to survey the SOS response kinetics. Simulation of LexA protein kinetics agrees with experimental data. We compare simulated LexA kinetic curves in wild-type and uνr - mutant bacteria, which is useful in investigating the way uνrABC-dependent excision repair modulates the SOS response kinetics. Possible applications of the model to investigating various aspects of the SOS induction are discussed

  11. A flow cytometry-optimized assay using an SOS-green fluorescent protein (SOS-GFP) whole-cell biosensor for the detection of genotoxins in complex environments

    DEFF Research Database (Denmark)

    Norman, Anders; Hansen, Lars H.; Sørensen, Søren Johannes

    2006-01-01

    /mL, and proved far more sensitive than a previously published assay using the same biosensor strain. By applying the SOS-green fluorescent protein (GFP) whole-cell biosensor directly to soil microcosms we were also able to evaluate both the applicability and sensitivity of a biosensor based on SOS...

  12. Oxidative stress is enhanced by hypothermia imposed on cerulein-induced pancreatitis in rats Aumento do estresse oxidativo após hipotermia em ratos com pancreatite induzida por ceruleína

    Directory of Open Access Journals (Sweden)

    Wellington Andraus

    2007-01-01

    com intervalo de uma hora. A amilase sérica, a permeabilidade vascular do pâncreas, a razão peso seco/peso úmido do pâncreas, a histopatologia e os níveis de glutationa foram analisados em cada grupo. RESULTADOS: Ratos hipotérmicos, com pancreatite aguda induzida por ceruleína, apresentaram maiores níveis de permeabilidade vascular no pâncreas (p < 0.05, razão peso seco/peso úmido do pâncreas (p = 0.03, e edema histológico (p < 0.05, mas os níveis de amilase sérica permaneceram iguais aos níveis apresentados pelos ratos normotérmicos. O grupo hipotérmico apresentou maior relação glutationa oxidada/glutationa reduzida em relação ao grupo normotérmico. CONCLUSÃO: Hipotermia moderada produziu uma maior resposta inflamatória em ratos com pancreatite aguda estabelecida, induzida por ceruleína, sugerindo que este efeito pode estar ligado a um maior índice de estresse oxidativo em ratos com pancreatite aguda.

  13. Avaliação da preferência alimentar em modelo de poliartrite induzida por colágeno

    OpenAIRE

    Eduarda Correa Freitas

    2014-01-01

    Introdução: Artrite reumatoide é uma doença inflamatória crônica associada a alterações no metabolismo proteico e energético. Objetivos: Investigar a preferência alimentar durante o desenvolvimento da inflamação crônica em ratas com artrite induzida por colágeno. Métodos: Ratas Wistar fêmeas foram alocadas em dois grupos: controle (CO, n=10) e artrite induzida por colágeno (CIA; n=11) e expostas simultaneamente a quatro diferentes dietas (padrão, hipercalórica, hiperlipidica e hiperproteica)....

  14. O Goodwill gerado pelo Passivo

    Directory of Open Access Journals (Sweden)

    Eliseu Martins

    2017-01-01

    Full Text Available Objetivo: O objetivo deste estudo está relacionado à figura do Goodwill originado do Passivo de uma empresa, ou seja, não é a do Goodwill normalmente constituído pelos Ativos Intangíveis capazes de produzir uma rentabilidade anormal e que fazem com que o valor da entidade como um todo, em funcionamento, seja maior do que a soma algébrica dos valores justos de seus elementos patrimoniais. Trata-se de uma visão que se pode entender como novidade para muitos, ou seja, a do Goodwill originado do Passivo e, não, dos Ativos de uma empresa. Existem empresas que sequer conseguem remunerar o risco que seus Ativos carregam, mas podem apresentar Goodwill devido à forma com que suas atividades são financiadas. Método: Como ensaio, são apresentados conceitos básicos e de formulação, normalmente utilizados em avaliações de empresas. Na sequência, são apresentadas simulações que demonstram, de forma objetiva, o verdadeiro sentido técnico da formulação. Resultados: Sabendo-se que determinadas agências estatais de desenvolvimento e o próprio BNDES acabam financiando determinadas atividades com taxas de juros generosas, este estudo apresenta ainda um caso real de existência de Goodwill originado do Passivo de uma empresa brasileira. Contribuições: Este trabalho tem como uma de suas principais contribuições a exposição de um tema que é praticamente desconhecido no mundo acadêmico, totalmente desconsiderado pelas normas e legislações contábeis, mas presente no mundo dos analistas e investidores mais bem preparados, e o faz pela não disseminação desse conceito, com apresentação e redação acessíveis a todos os níveis de leitores.

  15. Description and use of the SOS Plabord code

    International Nuclear Information System (INIS)

    Morera, J.P.; Samain, A.; Capes, H.; Ghendrih, P.

    1990-09-01

    The SOS Plabord code calculates the local steady states at the plasma edge. Plasma impurities and neutral particles freed from the wall are included in the calculations. The coordinates of the two axes that limit the plasma edge layer are defined in the program. Three sorts of ions and electrons are considered. The physical parameters, the equations and the boundary conditions are given. The method chosen for solving the nonlinear differential equations and the computer program are presented [fr

  16. Genetic analysis of the SOS response of Escherichia coli

    International Nuclear Information System (INIS)

    Mount, D.W.; Wertman, K.F.; Ennis, D.G.; Peterson, K.R.; Fisher, B.L.; Lyons, G.

    1983-01-01

    In the SOS response, a large number of E. coli genes having different functions are derepressed when the cellular DNA is damaged. This derepression occurs through inactivation of a repressor, the product of the lexA gene, by a protease activity of the recA gene product. The protease is thought to be activated in response to changes in DNA metabolism which follow the damage. After the SOS functions have acted, the protease activity declines and repression is again established. Because the DNA sequence of both lexA and recA have been determined, it is possible to induce many mutations in their regulatory and structural regions in order to analyze further the control of the SOS response. We are studying the effects of mutations in both the lexA and recA regulatory regions, and mutations which affect the protease activity or the sensitivity of repressor to the protease. Finally, we are using genetic methods to analyze a newly identified requirement for recA protein, induced mutagenesis in cells lacking repressor. 16 references, 3 figures

  17. Relação da perda auditiva induzida por ruído e o uso de tabaco em trabalhadores de uma indústria alimentícia

    Directory of Open Access Journals (Sweden)

    Eliziane Gai Menin

    2014-04-01

    Full Text Available Objetivo verificar se o uso do tabaco potencializa os efeitos do ruído causados na audição. Métodos 153 trabalhadores de ambos os sexos, fumantes e não-fumantes, de uma indústria do ramo alimentício, escolhidos aleatoriamente dentre 14 setores da empresa, cuja faixa de ruído apresentada teve uma variação de 85 a 109 dBNA, responderam a um questionário sobre tempo e exposição ao ruído bem como hábitos sobre fumo e passaram por exame de audiometria. Resultados os limiares auditivos da via aérea nas frequências de 4.000 Hz e 6.000Hz foram significantemente mais altos no grupo de fumantes/ex-fumantes quando comparados aos não-fumantes tanto na orelha direita quanto na orelha esquerda; limiares estes, característicos da perda auditiva induzida por ruído. Essas diferenças se mantiveram significantes após o ajuste pela idade e pelo tempo de exposição. Conclusão por meio dos resultados obtidos, concluiu-se que o uso do tabaco pode potencializar os danos causados pelo ruído à audição.

  18. SNAP/SOS: a package for simulating and analyzing safeguards systems

    International Nuclear Information System (INIS)

    Grant, F.H. III; Polito, J.; Sabuda, J.

    1983-01-01

    The effective analysis of safeguards systems at nuclear facilities requires significant effort. The Safeguards Network Analysis Procedure (SNAP) and the SNAP Operating System (SOS) reduce that effort to a manageable level. SNAP provides a detailed analysis of site safeguards for tactical evaluation. SOS helps the analyst organize and manage the SNAP effort effectively. SOS provides a database for model storage, automatic model generation, and computer graphics. The SOS/SNAP combination is a working example of a simulation system including executive-level control, database system, and facilities for model creation, editing, and output analysis

  19. Bevacizumab exacerbates sinusoidal obstruction syndrome (SOS) in the animal model and increases MMP 9 production.

    Science.gov (United States)

    Jafari, Azin; Matthaei, Hanno; Wehner, Sven; Tonguc, Tolga; Kalff, Jörg C; Manekeller, Steffen

    2018-04-24

    Thanks to modern multimodal treatment the ouctome of patients with colorectal cancer has experienced significant improvements. As a downside, agent specific side effects have been observed such as sinusoidal obstruction syndrome (SOS) after oxaliplatin chemotherapy (OX). Bevazicumab targeting VEGF is nowadays comprehensively used in combination protocols with OX but its impact on hepatotoxicity is thus far elusive and focus of the present study. After MCT administration 67% of animals developed SOS. GOT serum concentration significantly increased in animals developing SOS ( p SOS. In contrast, animals receiving VEGF developed SOS merely in 40% while increasing the VEGF dose led to a further decrease in SOS development to 25%. MMP 9 concentration in animals developing SOS was significantly higher compared to controls ( p SOS paralleled by MMP 9 production. Therefore, OX-Bevacizumab combination therapies should be administered with caution, especially if liver parenchyma damage is apparent. Male Sprague-Dawley rats were gavaged Monocrotaline (MCT) to induce SOS. Recombinant VEGF or an Anti-VEGF antibody was administered to MCT-treated rats and the hepatotoxic effect monitored in defined time intervals. MMP 9 expression in the liver was measured by ELISA.

  20. Effect of radiation doses rate on SOS response induction in irradiated Escherichia coli Cells

    International Nuclear Information System (INIS)

    Cuetara Lugo, Elizabeth B.; Fuentes Lorenzo, Jorge L.; Almeida Varela, Eliseo; Prieto Miranda, Enrique F.; Sanchez Lamar, Angel; Llagostera Casal, Montserrat

    2005-01-01

    The present work is aimed to study the effect of radiation dose rate on the induction of SOS response in Escherichia coli cells. We measured the induction of sul A reporter gene in PQ-37 (SOS Chromotest) cells. Lead devises were built with different diameter and these were used for diminishing the dose rate of PX- -30M irradiator. Our results show that radiation doses rate significantly modifies the induction of SOS response. Induction factor increases proportionally to doses rate in Escherichia coli cells defective to nucleotide excision repair (uvrA), but not in wild type cells. We conclude that the dose rate affects the level of induction of SOS response

  1. Cell death induced by tamoxifen in human blood lymphocytes cultivated in vitro = Morte celular induzida pelo tamoxifeno em linfócitos humanos cultivados in vitro

    Directory of Open Access Journals (Sweden)

    Selma Candelária Genari

    2010-10-01

    Full Text Available Many chemotherapeutic agents with a potential against solid tumors or leukemia can cause lymphopenia. Tamoxifen (TAM is a synthetic non-steroidal anti-estrogen drug employed in female breast cancer treatment. The present study investigated the capacity of TAM to induce cell death in human lymphocytes cultivated in vitro. Lymphocytes were obtained from young (25-30 years; n = 3 and elderly women (58-77 years; n = 3 and cultivated for 24 or 48h, with or without TAM (20 ƒÊM. After the culture, cell viability, immunocytochemical response and ultrastructure were evaluated. TAM affected lymphocytes in a time- dependent manner, and cells obtained from elderly women were the most sensitive to TAM. Immunocytochemicalanalysis evidenced higher frequency of apoptosis in treated cells, and the ultrastructural study revealed autophagic vacuoles, differing from the controls. In summary, the treated lymphocytes were affected by TAM, leading to cell death by apoptosis and autophagy.Muitos agentes quimioterapicos com potencial contra tumores solidos ou leucemias podem causar linfopenia. O Tamoxifeno (TAM e um agente antiestrogeno nao-esteroidal empregado no tratamento de cancer de mama feminino. O presente trabalho investigou a capacidade do TAM em induzir morte celular em linfocitos humanos cultivados in vitro. Oslinfocitos foram obtidos de mulheres jovens (25-30 anos; n = 3 e idosas (58-77 anos; n = 3 e cultivados por 24 ou 48h, com ou sem TAM (20 ƒÊM. Apos a cultura, foram analisadas a viabilidade celular, a resposta imunocitoquimica e a ultraestrutura. Os resultados indicam que o Tamoxifeno induziu morte celular em linfocitos de ambos os grupos, entretanto, as celulas das mulheres idosas apresentaram-se mais sensiveis ao tratamento. A analise imunocitoquimica mostrou maior frequencia de apoptose nas celulas tratadas e o estudo ultraestrutural revelou vacuolos autofagicos nos linfocitos expostos ao Tamoxifeno. Em conclusao, nosso estudo revelou que o TAM induziu morte celular por apoptose e autofagia.

  2. Única ou dupla inseminação artificial em tempo fixo em porcas com ovulações induzidas pelo Hormônio Luteinizante

    Directory of Open Access Journals (Sweden)

    Paulo Henrique Candini

    2004-04-01

    Full Text Available Duzentas e cinqüenta e quatro matrizes Camborough 22 (PIC®, foram divididas em 3 tratamentos: T 1 (n=60 - 600 UI de eCG após desmama e 5 mg de LH, 72 h após eCG , com única inseminação artificial (IA (24 h após LH; T 2 (n=95 - mesmo tratamento hormonal do T1, com 2 IA (24 e 32 h após LH; T 3 (n=99 - grupo controle sem tratamento hormonal, com 3 IA. As médias de intervalo desmame-estro (IDE em T1, T2 e T3 foram de 87,4 ± 3,0 (87 a 111, 87 ± 0 (87 e 99,9 ± 13,6 (63 a 135 horas, respectivamente, sendo reduzidas (P < 0,0001 pelas gonadotrofinas. A duração do estro (DE foi de 44,3 ± 8,78 (12 a 60, 41,3 ± 9,77 (24 a 60 e 60,1 ± 10,22 (36 a 84 ;horas;, respectivamente para T1, T2 e T3, sendo menor (P < 0,0001 nas fêmeas tratadas. As diferenças no intervalo LH e ovulação (LH-OV entre o grupo controle (56,1 ± 15,91, variação de 21 a 93 horas e os grupos tratados (35,7 ± 6,07 em T1 e 35,5 ± 6,06 em T2, com variação de 30 a 42 horas foram significativas (P < 0,0001. O tamanho de leitegada (TL foi de 10,6 ± 3,25 (2 a 16 em T1, 11,3 ± 3,0 (4 a 20 em T2 e 11,6 ± 2,74 (4 a 18 leitões em T3, enquanto os mesmos demonstraram número de leitões nascidos vivos (NV de 9,6 ± 3,14 (2 a 16, 10,5 ± 2,83 (1 a 18 e 10,5 ± 2,73 (4 a 16 leitões. Tanto em TL (P = 0,11 quanto em NV (P = 0,06 não houve diferença. A significante taxa de parição (TP no T1, T2 e T3 foi, respectivamente, 76,67 %, 88,42 % e 91,92 %, diferindo entre T1 e T3 (P = 0,01. As gonadotrofinas foram efetivas na indução e sincronização das ovulações; o uso de 2 IAs manteve os índices reprodutivos e, embora a IA única não revele significância quanto ao menor valor de TL, há necessidade de serem realizados novos estudos nesta área.

  3. Doença de depósito lisossomal induzida pelo consumo de Ipomoea verbascoidea (Convolvulaceae) em caprinos no semiárido de Pernambuco

    Science.gov (United States)

    The aim of this paper was to reproduce the poisoning of Ipomoea verbascoidea in goats and describe the epidemiological, clinical and pathological aspects of spontaneous poisoning by this plant in Pernambuco. For this, we studied the epidemiology of the disease in seven mu¬nicipalities in the semiari...

  4. Thymineless death is inhibited by CsrA in Escherichia coli lacking the SOS response.

    Science.gov (United States)

    Hamilton, Holly M; Wilson, Ray; Blythe, Martin; Nehring, Ralf B; Fonville, Natalie C; Louis, Edward J; Rosenberg, Susan M

    2013-11-01

    Thymineless death (TLD) is the rapid loss of colony-forming ability in bacterial, yeast and human cells starved for thymine, and is the mechanism of action of common chemotherapeutic drugs. In Escherichia coli, significant loss of viability during TLD requires the SOS replication-stress/DNA-damage response, specifically its role in inducing the inhibitor of cell division, SulA. An independent RecQ- and RecJ-dependent TLD pathway accounts for a similarly large additional component of TLD, and a third SOS- and RecQ/J-independent TLD pathway has also been observed. Although two groups have implicated the SOS-response in TLD, an SOS-deficient mutant strain from an earlier study was found to be sensitive to thymine deprivation. We performed whole-genome resequencing on that SOS-deficient strain and find that, compared with the SOS-proficient control strain, it contains five mutations in addition to the SOS-blocking lexA(Ind(-)) mutation. One of the additional mutations, csrA, confers TLD sensitivity specifically in SOS-defective strains. We find that CsrA, a carbon storage regulator, reduces TLD in SOS- or SulA-defective cells, and that the increased TLD that occurs in csrA(-) SOS-defective cells is dependent on RecQ. We consider a hypothesis in which the modulation of nucleotide pools by CsrA might inhibit TLD specifically in SOS-deficient (SulA-deficient) cells. Copyright © 2013 Elsevier B.V. All rights reserved.

  5. Analysis of the SOS response of Vibrio and other bacteria with multiple chromosomes.

    Science.gov (United States)

    Sanchez-Alberola, Neus; Campoy, Susana; Barbé, Jordi; Erill, Ivan

    2012-02-03

    The SOS response is a well-known regulatory network present in most bacteria and aimed at addressing DNA damage. It has also been linked extensively to stress-induced mutagenesis, virulence and the emergence and dissemination of antibiotic resistance determinants. Recently, the SOS response has been shown to regulate the activity of integrases in the chromosomal superintegrons of the Vibrionaceae, which encompasses a wide range of pathogenic species harboring multiple chromosomes. Here we combine in silico and in vitro techniques to perform a comparative genomics analysis of the SOS regulon in the Vibrionaceae, and we extend the methodology to map this transcriptional network in other bacterial species harboring multiple chromosomes. Our analysis provides the first comprehensive description of the SOS response in a family (Vibrionaceae) that includes major human pathogens. It also identifies several previously unreported members of the SOS transcriptional network, including two proteins of unknown function. The analysis of the SOS response in other bacterial species with multiple chromosomes uncovers additional regulon members and reveals that there is a conserved core of SOS genes, and that specialized additions to this basic network take place in different phylogenetic groups. Our results also indicate that across all groups the main elements of the SOS response are always found in the large chromosome, whereas specialized additions are found in the smaller chromosomes and plasmids. Our findings confirm that the SOS response of the Vibrionaceae is strongly linked with pathogenicity and dissemination of antibiotic resistance, and suggest that the characterization of the newly identified members of this regulon could provide key insights into the pathogenesis of Vibrio. The persistent location of key SOS genes in the large chromosome across several bacterial groups confirms that the SOS response plays an essential role in these organisms and sheds light into the

  6. CMOS/SOS 4k Rams hardened to 100 Krads (s:)

    International Nuclear Information System (INIS)

    Napoli, L.S.; Heagerty, W.F.; Smeltzer, R.K.; Yeh, J.L.

    1982-01-01

    Two CMOS/SOS 4K memories were fabricated with a recently developed, hardened SOS process. Memory functionality after radiation doses well in excess of 100 Krads(Si) was demonstrated. The critical device processing steps were identified. The radiationinduced failure mode of the memories is understood in terms of the circuit organization and the radiation behavior of the individual transistors in the memories

  7. Design rules for RCA self-aligned silicon-gate CMOS/SOS process

    Science.gov (United States)

    1977-01-01

    The CMOS/SOS design rules prepared by the RCA Solid State Technology Center (SSTC) are described. These rules specify the spacing and width requirements for each of the six design levels, the seventh level being used to define openings in the passivation level. An associated report, entitled Silicon-Gate CMOS/SOS Processing, provides further insight into the usage of these rules.

  8. Motility of Pseudomonas aeruginosa contributes to SOS-inducible biofilm formation.

    Science.gov (United States)

    Chellappa, Shakinah T; Maredia, Reshma; Phipps, Kara; Haskins, William E; Weitao, Tao

    2013-12-01

    DNA-damaging antibiotics such as ciprofloxacin induce biofilm formation and the SOS response through autocleavage of SOS-repressor LexA in Pseudomonas aeruginosa. However, the biofilm-SOS connection remains poorly understood. It was investigated with 96-well and lipid biofilm assays. The effects of ciprofloxacin were examined on biofilm stimulation of the SOS mutant and wild-type strains. The stimulation observed in the wild-type in which SOS was induced was reduced in the mutant in which LexA was made non-cleavable (LexAN) and thus SOS non-inducible. Therefore, the stimulation appeared to involve SOS. The possible mechanisms of inducible biofilm formation were explored by subproteomic analysis of outer membrane fractions extracted from biofilms. The data predicted an inhibitory role of LexA in flagellum function. This premise was tested first by functional and morphological analyses of flagellum-based motility. The flagellum swimming motility decreased in the LexAN strain treated with ciprofloxacin. Second, the motility-biofilm assay was performed, which tested cell migration and biofilm formation. The results showed that wild-type biofilm increased significantly over the LexAN. These results suggest that LexA repression of motility, which is the initial event in biofilm development, contributes to repression of SOS-inducible biofilm formation. Copyright © 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

  9. Lastekaitsepäeval avati Põltsamaal SOS-peremajad / Raivo Feldmann

    Index Scriptorium Estoniae

    Feldmann, Raivo

    2010-01-01

    Eesti teise SOS Lasteküla ametlikul avamisel Põltsamaal 1. juunil 2010. a. osalesid ka Norra suursaadik Eestis Stein Vegard Hagen ja SOS Lasteküla patroon proua Evelin Ilves. Presidendi abikaasa kinkis igale perele pereõunapuu ja koos kirjastusega Varrak igale peremajale väikese koduraamatukogu

  10. Suppression of the E. coli SOS response by dNTP pool changes.

    Science.gov (United States)

    Maslowska, Katarzyna H; Makiela-Dzbenska, Karolina; Fijalkowska, Iwona J; Schaaper, Roel M

    2015-04-30

    The Escherichia coli SOS system is a well-established model for the cellular response to DNA damage. Control of SOS depends largely on the RecA protein. When RecA is activated by single-stranded DNA in the presence of a nucleotide triphosphate cofactor, it mediates cleavage of the LexA repressor, leading to expression of the 30(+)-member SOS regulon. RecA activation generally requires the introduction of DNA damage. However, certain recA mutants, like recA730, bypass this requirement and display constitutive SOS expression as well as a spontaneous (SOS) mutator effect. Presently, we investigated the possible interaction between SOS and the cellular deoxynucleoside triphosphate (dNTP) pools. We found that dNTP pool changes caused by deficiencies in the ndk or dcd genes, encoding nucleoside diphosphate kinase and dCTP deaminase, respectively, had a strongly suppressive effect on constitutive SOS expression in recA730 strains. The suppression of the recA730 mutator effect was alleviated in a lexA-deficient background. Overall, the findings suggest a model in which the dNTP alterations in the ndk and dcd strains interfere with the activation of RecA, thereby preventing LexA cleavage and SOS induction. Published by Oxford University Press on behalf of Nucleic Acids Research 2015. This work is written by (a) US Government employee(s) and is in the public domain in the US.

  11. Validation of the Chinese Version of the Sense of Self (SOS) Scale

    Science.gov (United States)

    King, Ronnel B.; Ganotice, Fraide A., Jr.; Watkins, David A.

    2012-01-01

    This study explored the cross-cultural applicability of the Sense of Self (SOS) Scale in the Hong Kong Chinese cultural context. The SOS Scale is a 26-item questionnaire designed to measure students' sense of purpose, self-reliance, and self-concept in school. Six hundred ninety-seven Hong Kong Chinese high school students participated in the…

  12. Identification of a novel streptococcal gene cassette mediating SOS mutagenesis in Streptococcus uberis

    NARCIS (Netherlands)

    Varhimo, Emilia; Savijoki, Kirsi; Jalava, Jari; Kuipers, Oscar P.; Varmanen, Pekka

    Streptococci have been considered to lack the classical SOS response, defined by increased mutation after UV exposure and regulation by LexA. Here we report the identification of a potential self-regulated SOS mutagenesis gene cassette in the Streptococcaceae family. Exposure to UV light was found

  13. The complex between SOS3 and SOS2 regulatory domain from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis

    Energy Technology Data Exchange (ETDEWEB)

    Sánchez-Barrena, María José; Moreno-Pérez, Sandra; Angulo, Iván; Martínez-Ripoll, Martín; Albert, Armando, E-mail: xalbert@iqfr.csic.es [Grupo de Cristalografía Macromolecular y Biología Estructural, Instituto de Química Física ‘Rocasolano’, Consejo Superior de Investigaciones Científicas, Serrano 119, E-28006 Madrid (Spain)

    2007-07-01

    Recombinant SOS3 and SOS2 regulatory domain from A. thaliana have been coexpressed in E. coli, purified and crystallized by the hanging-drop vapour-diffusion method. An X-ray data set has been collected at 2.0 Å resolution. The salt-tolerance genes SOS3 (salt overly sensitive 3) and SOS2 (salt overly sensitive 2) regulatory domain of Arabidopsis thaliana were cloned into a polycistronic plasmid and the protein complex was expressed in Escherichia coli, allowing purification to homogeneity in three chromatographic steps. Crystals were grown using vapour-diffusion techniques. The crystals belonged to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 44.14, b = 57.39, c = 141.90 Å.

  14. Efeito antioxidante da buspirona no modelo de epilepsia induzida por pilocarpina

    Directory of Open Access Journals (Sweden)

    Paula Benvindo Ferreira

    2012-01-01

    Full Text Available CONTEXTO: Crises epilépticas induzidas por pilocarpina podem produzir alterações histopatológicas em muitas regiões cerebrais como consequência da produção excessiva de radicais livres. OBJETIVO: O objetivo do presente estudo foi avaliar o efeito antioxidante da buspirona no modelo de epilepsia induzida por pilocarpina. MATERIAL E MÉTODOS: Quarenta e oito animais foram divididos em quatro grupos. O primeiro grupo foi tratado com solução salina 0,9% (Controle. O segundo grupo foi tratado com pilocarpina 400 mg/kg (P400. Por sua vez, o terceiro grupo foi tratado com buspirona 5 mg/kg (BUSP durante 14 dias consecutivos. Já os animais do quarto grupo foram tratados com buspirona durante 14 dias consecutivos, e, 30 minutos após a última administração dela, os camundongos receberam P400 (BUSP + P400. RESULTADOS: Durante o período do tratamento não se observaram sinais de toxicidade e nenhuma morte entre os animais tratados com buspirona. Em nosso estudo o grupo tratado com P400 demonstrou um aumento significativo da produção de nitrito e nos níveis de peroxidação lipídica após as crises epilépticas. Por outro lado, no hipocampo dos animais que receberam o pré-tratamento com buspirona e após 30 minutos receberam P400, foi observada redução significativa nos níveis de peroxidação lipídica (65% e nitrito (85%, bem como aumento na atividade da enzima superóxido dismutase. CONCLUSÃO: O pré-tratamento com BUSP aumentou a latência para primeira crise epiléptica e diminuiu a taxa de mortalidade e o número de animais que apresentaram crise epiléptica e que progridem para o estado de mal epiléptico. Além disso, apresentou efeitos anticonvulsivantes associados com a redução do estresse oxidativo hipocampal no modelo de epilepsia induzida por pilocarpina.

  15. 77 FR 65896 - Award of a Single-Source Replacement Grant to SOS Children's Villages Illinois in Chicago, IL

    Science.gov (United States)

    2012-10-31

    ....623] Award of a Single-Source Replacement Grant to SOS Children's Villages Illinois in Chicago, IL... (FYSB) announces the award of a single-source replacement grant to SOS Children's Villages Illinois in... grant. ACYF/FYSB has designated SOS Children's Villages Illinois, a 501(c)(3) non-profit organization...

  16. Molecular kinetics. Ras activation by SOS: allosteric regulation by altered fluctuation dynamics.

    Science.gov (United States)

    Iversen, Lars; Tu, Hsiung-Lin; Lin, Wan-Chen; Christensen, Sune M; Abel, Steven M; Iwig, Jeff; Wu, Hung-Jen; Gureasko, Jodi; Rhodes, Christopher; Petit, Rebecca S; Hansen, Scott D; Thill, Peter; Yu, Cheng-Han; Stamou, Dimitrios; Chakraborty, Arup K; Kuriyan, John; Groves, Jay T

    2014-07-04

    Activation of the small guanosine triphosphatase H-Ras by the exchange factor Son of Sevenless (SOS) is an important hub for signal transduction. Multiple layers of regulation, through protein and membrane interactions, govern activity of SOS. We characterized the specific activity of individual SOS molecules catalyzing nucleotide exchange in H-Ras. Single-molecule kinetic traces revealed that SOS samples a broad distribution of turnover rates through stochastic fluctuations between distinct, long-lived (more than 100 seconds), functional states. The expected allosteric activation of SOS by Ras-guanosine triphosphate (GTP) was conspicuously absent in the mean rate. However, fluctuations into highly active states were modulated by Ras-GTP. This reveals a mechanism in which functional output may be determined by the dynamical spectrum of rates sampled by a small number of enzymes, rather than the ensemble average. Copyright © 2014, American Association for the Advancement of Science.

  17. [Expressions of Ras and Sos1 in epithelial ovarian cancer tissues and their clinical significance].

    Science.gov (United States)

    Xiao, Zheng-Hua; Linghu, Hua; Liu, Qian-Fen

    2016-11-20

    To detect the expressions of Ras and Sos1 proteins in human epithelial ovarian cancer (EOC) tissues and explore their correlation with the clinicopathological features of the patients. The expressions of Ras and Sos1 proteins were detected immunohistochemically in 62 EOC tissues, 5 borderline ovarian cancer tissues, 15 benign epithelial ovarian neoplasm tissues, and 18 normal ovarian tissues. The EOC tissues showed significantly higher expression levels of both Ras and Sos1 than the other tissues tested (Ptissues, Ras and Sos1 proteins were expressed mostly on the cell membrane and in the cytoplasm. The expression level of Ras was correlated with pathological types of the tumor (Ptissue-specific variation of Ras expression can lend support to a specific diagnosis of ovarian serous adenocarcinoma. The association of Ras and Sos1 protein expression with the tumor-free survival time of the patients awaits further investigation with a larger sample size.

  18. O MARKETING ORIENTADO PELO MERCADO

    Directory of Open Access Journals (Sweden)

    Sandro Deretti

    2005-11-01

    -style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} -->

    Resumo

    A economia globalizada determinou alterações profundas no processo estratégico das empresas, propiciando oportunidades e ameaças em todos os segmentos e exigindo produtos e serviços competitivos. Assim, diante deste cenário extremamente desafiador, a função marketing dentro das empresas assume maior importância no desenvolvimento de vantagens competitivas sustentáveis e na redução das incertezas. Muitos autores consideram que a orientação para o mercado seja a essência da filosofia empresarial que leva ao sucesso nesta luta pelos mercados porque investiga desejos e necessidades do consumidor. Este artigo faz uma contextualização dos principais trabalhos que colaboraram para a estruturação do conceito de marketing e da orientação para o mercado.

     

  19. Zinc blocks SOS-induced antibiotic resistance via inhibition of RecA in Escherichia coli.

    Science.gov (United States)

    Bunnell, Bryan E; Escobar, Jillian F; Bair, Kirsten L; Sutton, Mark D; Crane, John K

    2017-01-01

    Zinc inhibits the virulence of diarrheagenic E. coli by inducing the envelope stress response and inhibiting the SOS response. The SOS response is triggered by damage to bacterial DNA. In Shiga-toxigenic E. coli, the SOS response strongly induces the production of Shiga toxins (Stx) and of the bacteriophages that encode the Stx genes. In E. coli, induction of the SOS response is accompanied by a higher mutation rate, called the mutator response, caused by a shift to error-prone DNA polymerases when DNA damage is too severe to be repaired by canonical DNA polymerases. Since zinc inhibited the other aspects of the SOS response, we hypothesized that zinc would also inhibit the mutator response, also known as hypermutation. We explored various different experimental paradigms to induce hypermutation triggered by the SOS response, and found that hypermutation was induced not just by classical inducers such as mitomycin C and the quinolone antibiotics, but also by antiviral drugs such as zidovudine and anti-cancer drugs such as 5-fluorouracil, 6-mercaptopurine, and azacytidine. Zinc salts inhibited the SOS response and the hypermutator phenomenon in E. coli as well as in Klebsiella pneumoniae, and was more effective in inhibiting the SOS response than other metals. We then attempted to determine the mechanism by which zinc, applied externally in the medium, inhibits hypermutation. Our results show that zinc interferes with the actions of RecA, and protects LexA from RecA-mediated cleavage, an early step in initiation of the SOS response. The SOS response may play a role in the development of antibiotic resistance and the effect of zinc suggests ways to prevent it.

  20. Specificity in suppression of SOS expression by recA4162 and uvrD303.

    Science.gov (United States)

    Massoni, Shawn C; Sandler, Steven J

    2013-12-01

    Detection and repair of DNA damage is essential in all organisms and depends on the ability of proteins recognizing and processing specific DNA substrates. In E. coli, the RecA protein forms a filament on single-stranded DNA (ssDNA) produced by DNA damage and induces the SOS response. Previous work has shown that one type of recA mutation (e.g., recA4162 (I298V)) and one type of uvrD mutation (e.g., uvrD303 (D403A, D404A)) can differentially decrease SOS expression depending on the type of inducing treatments (UV damage versus RecA mutants that constitutively express SOS). Here it is tested using other SOS inducing conditions if there is a general feature of ssDNA generated during these treatments that allows recA4162 and uvrD303 to decrease SOS expression. The SOS inducing conditions tested include growing cells containing temperature-sensitive DNA replication mutations (dnaE486, dnaG2903, dnaN159, dnaZ2016 (at 37°C)), a del(polA)501 mutation and induction of Double-Strand Breaks (DSBs). uvrD303 could decrease SOS expression under all conditions, while recA4162 could decrease SOS expression under all conditions except in the polA strain or when DSBs occur. It is hypothesized that recA4162 suppresses SOS expression best when the ssDNA occurs at a gap and that uvrD303 is able to decrease SOS expression when the ssDNA is either at a gap or when it is generated at a DSB (but does so better at a gap). Copyright © 2013 Elsevier B.V. All rights reserved.

  1. Zinc blocks SOS-induced antibiotic resistance via inhibition of RecA in Escherichia coli.

    Directory of Open Access Journals (Sweden)

    Bryan E Bunnell

    Full Text Available Zinc inhibits the virulence of diarrheagenic E. coli by inducing the envelope stress response and inhibiting the SOS response. The SOS response is triggered by damage to bacterial DNA. In Shiga-toxigenic E. coli, the SOS response strongly induces the production of Shiga toxins (Stx and of the bacteriophages that encode the Stx genes. In E. coli, induction of the SOS response is accompanied by a higher mutation rate, called the mutator response, caused by a shift to error-prone DNA polymerases when DNA damage is too severe to be repaired by canonical DNA polymerases. Since zinc inhibited the other aspects of the SOS response, we hypothesized that zinc would also inhibit the mutator response, also known as hypermutation. We explored various different experimental paradigms to induce hypermutation triggered by the SOS response, and found that hypermutation was induced not just by classical inducers such as mitomycin C and the quinolone antibiotics, but also by antiviral drugs such as zidovudine and anti-cancer drugs such as 5-fluorouracil, 6-mercaptopurine, and azacytidine. Zinc salts inhibited the SOS response and the hypermutator phenomenon in E. coli as well as in Klebsiella pneumoniae, and was more effective in inhibiting the SOS response than other metals. We then attempted to determine the mechanism by which zinc, applied externally in the medium, inhibits hypermutation. Our results show that zinc interferes with the actions of RecA, and protects LexA from RecA-mediated cleavage, an early step in initiation of the SOS response. The SOS response may play a role in the development of antibiotic resistance and the effect of zinc suggests ways to prevent it.

  2. Condutividade induzida por radiação ionizante no Mylar (PET) e Kapton (polimiidia).

    OpenAIRE

    Rinaldo Gregório Filho

    1986-01-01

    Este trabalho apresenta uma extensiva série de resultados experimentais da condutividade induzida por radiação X contínua, durante e após a irradiação, em amostras de PET e Kapton. As medidas foram realizadas variando-se uma série de parâmetros, tais como: o campo elétrico aplicado, a taxa de exposição, a espessura da amostra, o tipo de eletrodo, a energia da radiação e as condições ambientes. Foram feitas ainda medidas da corrente termo-estimulada em amostras irradiadas e não irradiadas, que...

  3. El último urbanismo de Antonio Bonet: el poblado SOS (1970

    Directory of Open Access Journals (Sweden)

    Juan Fernando Ródenas García

    2018-04-01

    Full Text Available El poblado SOS de Aldeas Infantiles, Sant Feliu de Codines, Barcelona (1970, junto al poblado Hifrensa (realizado y los planes urbanísticos de Prat I y II (no realizados, constituyen los últimos conjuntos urbanísticos de cierto calado proyectados por Antonio Bonet, si exceptuamos su producción turística. Bonet plantea un conjunto residencial para alojar a niños huérfanos con equipamientos comunitarios educacionales y deportivos. Bonet recrea en el poblado SOS la atmósfera, a escala humana, que se respira en aquellos pueblos que aparecen fotografiados en el número 18 (1935 de la revista del GATEPAC, AC Documentos de Actividad Contemporánea, dedicado a la arquitectura popular. Pabellones encalados, bóvedas, porches, patios, muros y plataformas de piedra dispuestas como bancales agrícolas, constituyen los elementos que construyen el paisaje de un poblado de trazo moderno y formas arcaicas. Se propone el análisis de una obra inédita que, aunque no se llevó a cabo, expresa la singular lectura del autor de las condiciones de habitabilidad para niños huérfanos, del paisaje, y al mismo tiempo, la obra condensa la experiencia de Bonet como urbanista ya experimentado que en los años 70 pone a prueba con perspectiva histórica su credo teórico fundamental: la Carta de Atenas.

  4. Efeitos de alendronato de sódio, atorvastatina cálcica e ipriflavona sobre a osteoporose induzida com dexametasona em ratas

    Directory of Open Access Journals (Sweden)

    A. S. PINTO

    2009-01-01

    Full Text Available

    O crescente aumento da prevalência da osteoporose tem sido considerado um grave problema de saúde pública no mundo. A osteoporose é uma doença caracterizada por diminuição da massa óssea, com conseqüente aumento do risco de fraturas, necessitando, portanto, de métodos preventivos e de tratamentos eficazes. O presente trabalho teve como objetivo verificar, comparativamente, os efeitos do bifosfonato alendronato de sódio, da estatina atorvastatina cálcica e do flavonóide ipriflavona sobre a osteoporose induzida pelo glicocorticóide dexametasona em ratas. Os efeitos desses fármacos foram avaliados pelos marcadores bioquímicos cálcio e fósforo sérico, fosfatase alcalina óssea e exame histomorfométrico. Os resultados obtidos a partir da análise dos marcadores bioquímicos não foram significativos, não fornecendo subsídios para o diagnóstico e acompanhamento do tratamento da osteoporose. No entanto, a avaliação histomorfométrica permitiu a análise estática e dinâmica, bem como detecção de alterações teciduais na unidade metabólica óssea, particularmente, no osso trabecular. Verificou-se que os tratamentos testados determinaram resultados significativos no aumento da densidade trabecular óssea, destacando-se o bifosfonato que apresentou o melhor resultado, alcançando níveis de densidade trabecular óssea semelhantes aos dos animais intactos. Além disso, a histomorfometria se mostrou válida na detecção da osteoporose, podendo servir de modelo para o estudo de drogas sobre este distúrbio ósteo-metabólico. Palavras-chave: Osteoporose, dexametasona, alendronato, atorvastatina, ipriflavona.

  5. Systematically Altering Bacterial SOS Activity under Stress Reveals Therapeutic Strategies for Potentiating Antibiotics.

    Science.gov (United States)

    Mo, Charlie Y; Manning, Sara A; Roggiani, Manuela; Culyba, Matthew J; Samuels, Amanda N; Sniegowski, Paul D; Goulian, Mark; Kohli, Rahul M

    2016-01-01

    The bacterial SOS response is a DNA damage repair network that is strongly implicated in both survival and acquired drug resistance under antimicrobial stress. The two SOS regulators, LexA and RecA, have therefore emerged as potential targets for adjuvant therapies aimed at combating resistance, although many open questions remain. For example, it is not well understood whether SOS hyperactivation is a viable therapeutic approach or whether LexA or RecA is a better target. Furthermore, it is important to determine which antimicrobials could serve as the best treatment partners with SOS-targeting adjuvants. Here we derived Escherichia coli strains that have mutations in either lexA or recA genes in order to cover the full spectrum of possible SOS activity levels. We then systematically analyzed a wide range of antimicrobials by comparing the mean inhibitory concentrations (MICs) and induced mutation rates for each drug-strain combination. We first show that significant changes in MICs are largely confined to DNA-damaging antibiotics, with strains containing a constitutively repressed SOS response impacted to a greater extent than hyperactivated strains. Second, antibiotic-induced mutation rates were suppressed when SOS activity was reduced, and this trend was observed across a wider spectrum of antibiotics. Finally, perturbing either LexA or RecA proved to be equally viable strategies for targeting the SOS response. Our work provides support for multiple adjuvant strategies, while also suggesting that the combination of an SOS inhibitor with a DNA-damaging antibiotic could offer the best potential for lowering MICs and decreasing acquired drug resistance. IMPORTANCE Our antibiotic arsenal is becoming depleted, in part, because bacteria have the ability to rapidly adapt and acquire resistance to our best agents. The SOS pathway, a widely conserved DNA damage stress response in bacteria, is activated by many antibiotics and has been shown to play central role in

  6. Characterization of Salt Overly Sensitive 1 (SOS1) gene homoeologs in quinoa (Chenopodium quinoa Willd.).

    Science.gov (United States)

    Maughan, P J; Turner, T B; Coleman, C E; Elzinga, D B; Jellen, E N; Morales, J A; Udall, J A; Fairbanks, D J; Bonifacio, A

    2009-07-01

    Salt tolerance is an agronomically important trait that affects plant species around the globe. The Salt Overly Sensitive 1 (SOS1) gene encodes a plasma membrane Na+/H+ antiporter that plays an important role in germination and growth of plants in saline environments. Quinoa (Chenopodium quinoa Willd.) is a halophytic, allotetraploid grain crop of the family Amaranthaceae with impressive nutritional content and an increasing worldwide market. Many quinoa varieties have considerable salt tolerance, and research suggests quinoa may utilize novel mechanisms to confer salt tolerance. Here we report the cloning and characterization of two homoeologous SOS1 loci (cqSOS1A and cqSOS1B) from C. quinoa, including full-length cDNA sequences, genomic sequences, relative expression levels, fluorescent in situ hybridization (FISH) analysis, and a phylogenetic analysis of SOS1 genes from 13 plant taxa. The cqSOS1A and cqSOS1B genes each span 23 exons spread over 3477 bp and 3486 bp of coding sequence, respectively. These sequences share a high level of similarity with SOS1 homologs of other species and contain two conserved domains, a Nhap cation-antiporter domain and a cyclic-nucleotide binding domain. Genomic sequence analysis of two BAC clones (98 357 bp and 132 770 bp) containing the homoeologous SOS1 genes suggests possible conservation of synteny across the C. quinoa sub-genomes. This report represents the first molecular characterization of salt-tolerance genes in a halophytic species in the Amaranthaceae as well as the first comparative analysis of coding and non-coding DNA sequences of the two homoeologous genomes of C. quinoa.

  7. Suppressive effects of coffee on the SOS responses induced by UV and chemical mutagens

    International Nuclear Information System (INIS)

    Obana, Hirotaka; Nakamura, Sei-ichi; Tanaka, Ryou-ichi

    1986-01-01

    SOS-inducing activity of UV or chemical mutagens was strongly suppressed by instant coffee in Salmonella typhimurium TA1535/pSK1002. As decaffeinated instant coffee showed a similarly strong suppressive effect, it would seem that caffeine, a known inhibitor of SOS responses, is not responsible for the effect observed. The suppression was also shown by freshly brewed coffee extracts. However, the suppression was absent in green coffee-bean extracts. These results suggest that coffee contains some substance(s) which, apart from caffeine, suppresses SOS-inducing activity of UV or chemical mutagens and that the suppressive substance(s) are produced by roasting coffee beans. (Auth.)

  8. Endovascular stentectomy using the snare over stent-retriever (SOS technique: An experimental feasibility study.

    Directory of Open Access Journals (Sweden)

    Tareq Meyer

    Full Text Available Feasibility of endovascular stentectomy using a snare over stent-retriever (SOS technique was evaluated in a silicon flow model and an in vivo swine model. In vitro, stentectomy of different intracranial stents using the SOS technique was feasible in 22 out of 24 (92% retrieval maneuvers. In vivo, stentectomy was successful in 10 out of 10 procedures (100%. In one case self-limiting vasospasm was observed angiographically as a technique related complication in the animal model. Endovascular stentectomy using the SOS technique is feasible in an experimental setting and may be transferred to a clinical scenario.

  9. The SOS model partition function and the elliptic weight functions

    International Nuclear Information System (INIS)

    Pakuliak, S; Silantyev, A; Rubtsov, V

    2008-01-01

    We generalized a recent observation (Khoroshkin and Pakuliak 2005 Theor. Math. Phys. 145 1373) that the partition function of the six-vertex model with domain wall boundary conditions can be obtained from a calculation of projections of the product of total currents in the quantum affine algebra U q (sl 2 -hat) in its current realization. A generalization is done for the elliptic current algebra (Enriquez and Felder 1998 Commun. Math. Phys. 195 651, Enriquez and Rubtsov 1997 Ann. Sci. Ecole Norm. Sup. 30 821). The projections of the product of total currents in this case are calculated explicitly and are presented as integral transforms of a product of the total currents. It is proved that the integral kernel of this transform is proportional to the partition function of the SOS model with domain wall boundary conditions

  10. NKS/SOS-1 seminar on safety analysis

    Energy Technology Data Exchange (ETDEWEB)

    Lauridsen, K. [Risoe National Lab., Roskilde (Denmark); Anderson, K. [Karinta-Konsult (Sweden); Pulkkinen, U. [VTT Automation (Finland)

    2001-05-01

    The report describes presentations and discussions at a seminar held at Risoe on March 22-23, 2000. The title of the seminar was NKS/SOS-1 - Safety Analysis. It dealt with issues of relevance for the safety analysis for the entire nuclear safety field (notably reactors and nuclear waste repositories). Such issues were: objectives of safety analysis, risk criteria, decision analysis, expert judgement and risk communication. In addition, one talk dealt with criteria for chemical industries in Europe. The seminar clearly showed that the concept of risk is multidimensional, which makes clarity and transparency essential elements in risk communication, and that there are issues of common concern between different applications, such as how to deal with different kinds of uncertainty and expert judgement. (au)

  11. Selective Osmotic Shock (SOS)-Based Islet Isolation for Microencapsulation.

    Science.gov (United States)

    Enck, Kevin; McQuilling, John Patrick; Orlando, Giuseppe; Tamburrini, Riccardo; Sivanandane, Sittadjody; Opara, Emmanuel C

    2017-01-01

    Islet transplantation (IT) has recently been shown to be a promising alternative to pancreas transplantation for reversing diabetes. IT requires the isolation of the islets from the pancreas, and these islets can be used to fabricate a bio-artificial pancreas. Enzymatic digestion is the current gold standard procedure for islet isolation but has lingering concerns. One such concern is that it has been shown to damage the islets due to nonselective tissue digestion. This chapter provides a detailed description of a nonenzymatic method that we are exploring in our lab as an alternative to current enzymatic digestion procedures for islet isolation from human and nonhuman pancreatic tissues. This method is based on selective destruction and protection of specific cell types and has been shown to leave the extracellular matrix (ECM) of islets intact, which may thus enhance islet viability and functionality. We also show that these SOS-isolated islets can be microencapsulated for transplantation.

  12. The Self-help Online against Suicidal thoughts (SOS) trial

    DEFF Research Database (Denmark)

    Mühlmann, Charlotte; Madsen, Trine; Hjorthøj, Carsten Rygaard

    2017-01-01

    -list assignment for 32 weeks. The primary outcomes are frequency and intensity of suicidal thoughts. Secondary outcome measures include depressive symptoms, hopelessness, worrying, quality of life, costs related to health care utilization and production loss. Number of deliberate self-harm episodes, suicides......BACKGROUND: Suicidal thoughts are common, causing distress for millions of people all over the world. However, people with suicidal thoughts might not access support due to financial restraints, stigma or a lack of available treatment offers. Self-help programs provided online could overcome...... these barriers, and previous efforts show promising results in terms of reducing suicidal thoughts. This study aims to examine the effectiveness of an online self-help intervention in reducing suicidal thoughts among people at risk of suicide. The Danish Self-help Online against Suicidal thoughts (SOS) trial...

  13. Radiation-hardened CMOS/SOS LSI circuits

    International Nuclear Information System (INIS)

    Aubuchon, K.G.; Peterson, H.T.; Shumake, D.P.

    1976-01-01

    The recently developed technology for building radiation-hardened CMOS/SOS devices has now been applied to the fabrication of LSI circuits. This paper describes and presents results on three different circuits: an 8-bit adder/subtractor (Al gate), a 256-bit shift register (Si gate), and a polycode generator (Al gate). The 256-bit shift register shows very little degradation after 1 x 10 6 rads (Si), with an increase from 1.9V to 2.9V in minimum operating voltage, a decrease of about 20% in maximum frequency, and little or no change in quiescent current. The p-channel thresholds increase from -0.9V to -1.3V, while the n-channel thresholds decrease from 1.05 to 0.23V, and the n-channel leakage remains below 1nA/mil. Excellent hardening results were also obtained on the polycode generator circuit. Ten circuits were irradiated to 1 x 10 6 rads (Si), and all continued to function well, with an increase in minimum power supply voltage from 2.85V to 5.85V and an increase in quiescent current by a factor of about 2. Similar hardening results were obtained on the 8-bit adder, with the minimum power supply voltage increasing from 2.2V to 4.6V and the add time increasing from 270 to 350 nsec after 1 x 10 6 rads (Si). These results show that large CMOS/SOS circuits can be hardened to above 1 x 10 6 rads (Si) with either the Si gate or Al gate technology. The paper also discusses the relative advantages of the Si gate versus the Al gate technology

  14. Dyskinesia induced by phenytoin Discinesia induzida por fenitoína

    Directory of Open Access Journals (Sweden)

    M. AUGUSTA MONTENEGRO

    1999-06-01

    Full Text Available Phenytoin is an effective antiepileptic drug, although, it can be associated with many side effects, including dyskinesia. OBJECTIVE: To describe the clinical characteristics of phenytoin induced dyskinesia. METHODS: We investigated the occurrence of involuntary movements in patients followed at our adult and pediatric epilepsy clinics during the period of one year. RESULTS: Three patients presented with phenytoin-induced dyskinesia: one adult with axial and orofacial dyskinesia, and two children with choreoathetosis. They did not have other signs of phenytoin intoxication and had complete recovery after phenytoin withdrawal. CONCLUSION: Phenytoin induced dyskinesia may occur during either chronic or initial treatment and with normal serum phenytoin levels. However, it occurs most often in patients on polytherapy, usually after increasing dosage and with toxic serum levels. Other signs of phenytoin intoxication may be present in these patients, but often the dyskinesia is the only side effect, which may delay the diagnosis and treatment. The clinical characteristics of the involuntary movements vary and may be focal or generalized, most often characterized by choreoathetosis and dyskinesias. These may last for hours, days or even years, but frequently disappear completely after phenytoin withdrawal.Fenitoína é droga anti-epiléptica eficaz, mas pode estar associada a vários efeitos colaterais, inclusive discinesia. OBJETIVO: Descrever as características clínicas da discinesia induzida por fenitoína. MÉTODO: Avaliamos a ocorrência de movimentos involuntários em pacientes seguidos nos ambulatórios de epilepsia durante o período de um ano. RESULTADOS: Três pacientes apresentaram discinesia induzida por fenitoína: um adulto com discinesia orofacial e duas crianças com coreoatetose. Eles não tinham outros sinais de intoxicação por fenitoína e apresentaram recuperação completa após a retirada da fenitoína. CONCLUSÃO: Discinesia

  15. Gain-of-function SOS1 mutations cause a distinctive form of noonansyndrome

    Energy Technology Data Exchange (ETDEWEB)

    Tartaglia, Marco; Pennacchio, Len A.; Zhao, Chen; Yadav, KamleshK.; Fodale, Valentina; Sarkozy, Anna; Pandit, Bhaswati; Oishi, Kimihiko; Martinelli, Simone; Schackwitz, Wendy; Ustaszewska, Anna; Martin, Joes; Bristow, James; Carta, Claudio; Lepri, Francesca; Neri, Cinzia; Vasta,Isabella; Gibson, Kate; Curry, Cynthia J.; Lopez Siguero, Juan Pedro; Digilio, Maria Cristina; Zampino, Giuseppe; Dallapiccola, Bruno; Bar-Sagi, Dafna; Gelb, Brude D.

    2006-09-01

    Noonan syndrome (NS) is a developmental disordercharacterized by short stature, facial dysmorphia, congenital heartdefects and skeletal anomalies1. Increased RAS-mitogenactivated proteinkinase (MAPK) signaling due to PTPN11 and KRAS mutations cause 50 percentof NS2-6. Here, we report that 22 of 129 NS patients without PTPN11 orKRAS mutation (17 percent) have missense mutations in SOS1, which encodesa RAS-specific guanine nucleotide exchange factor (GEF). SOS1 mutationscluster at residues implicated in the maintenance of SOS1 in itsautoinhibited form and ectopic expression of two NS-associated mutantsinduced enhanced RAS activation. The phenotype associated with SOS1defects is distinctive, although within NS spectrum, with a highprevalence of ectodermal abnormalities but generally normal developmentand linear growth. Our findings implicate for the first timegain-of-function mutations in a RAS GEF in inherited disease and define anew mechanism by which upregulation of the RAS pathway can profoundlychange human development.

  16. Characterization of the SOS meta-regulon in the human gut microbiome.

    Science.gov (United States)

    Cornish, Joseph P; Sanchez-Alberola, Neus; O'Neill, Patrick K; O'Keefe, Ronald; Gheba, Jameel; Erill, Ivan

    2014-05-01

    Data from metagenomics projects remain largely untapped for the analysis of transcriptional regulatory networks. Here, we provide proof-of-concept that metagenomic data can be effectively leveraged to analyze regulatory networks by characterizing the SOS meta-regulon in the human gut microbiome. We combine well-established in silico and in vitro techniques to mine the human gut microbiome data and determine the relative composition of the SOS network in a natural setting. Our analysis highlights the importance of translesion synthesis as a primary function of the SOS response. We predict the association of this network with three novel protein clusters involved in cell wall biogenesis, chromosome partitioning and restriction modification, and we confirm binding of the SOS response transcriptional repressor to sites in the promoter of a cell wall biogenesis enzyme, a phage integrase and a death-on-curing protein. We discuss the implications of these findings and the potential for this approach for metagenome analysis.

  17. Evelin Ilves ja kirjastus "Varrak" kinkisid SOS Lastekülale jõuludeks raamatuid

    Index Scriptorium Estoniae

    2009-01-01

    Proua Evelin Ilves ja kirjastus "Varrak" viisid 21. detsembril 2009 Keila SOS Lastekülale jõulukingiks raamatuid. Kingitud raamatud valiti välja laste soovide põhjal, nende hulgas on nii lastekirjandust kui ka teatmeteoseid

  18. Analysis of the SOS response of Vibrio and other bacteria with multiple chromosomes

    Directory of Open Access Journals (Sweden)

    Sanchez-Alberola Neus

    2012-02-01

    Full Text Available Abstract Background The SOS response is a well-known regulatory network present in most bacteria and aimed at addressing DNA damage. It has also been linked extensively to stress-induced mutagenesis, virulence and the emergence and dissemination of antibiotic resistance determinants. Recently, the SOS response has been shown to regulate the activity of integrases in the chromosomal superintegrons of the Vibrionaceae, which encompasses a wide range of pathogenic species harboring multiple chromosomes. Here we combine in silico and in vitro techniques to perform a comparative genomics analysis of the SOS regulon in the Vibrionaceae, and we extend the methodology to map this transcriptional network in other bacterial species harboring multiple chromosomes. Results Our analysis provides the first comprehensive description of the SOS response in a family (Vibrionaceae that includes major human pathogens. It also identifies several previously unreported members of the SOS transcriptional network, including two proteins of unknown function. The analysis of the SOS response in other bacterial species with multiple chromosomes uncovers additional regulon members and reveals that there is a conserved core of SOS genes, and that specialized additions to this basic network take place in different phylogenetic groups. Our results also indicate that across all groups the main elements of the SOS response are always found in the large chromosome, whereas specialized additions are found in the smaller chromosomes and plasmids. Conclusions Our findings confirm that the SOS response of the Vibrionaceae is strongly linked with pathogenicity and dissemination of antibiotic resistance, and suggest that the characterization of the newly identified members of this regulon could provide key insights into the pathogenesis of Vibrio. The persistent location of key SOS genes in the large chromosome across several bacterial groups confirms that the SOS response plays an

  19. Physical modeling of SOS P channel MOSFET and comparison with bulk devices

    International Nuclear Information System (INIS)

    Merckel, G.; Gris, Y.

    1976-01-01

    The main technological steps applied to P channel MOSFET's on SOS are recalled. A large-signal model derived from a physical analysis is presented. Gate-source and gate-drain capacitors have been linearized versus drain voltage. Due to low injection, the only diffusion capacitance of the source-substrate forward biased diode, and the depletion capacitance of the drain-substrate reverse biased diode were taken into account. Some typical parameters measured on SOS and bulk devices are given [fr

  20. Integral formula for elliptic SOS models with domain walls and a reflecting end

    Energy Technology Data Exchange (ETDEWEB)

    Lamers, Jules, E-mail: j.lamers@uu.nl

    2015-12-15

    In this paper we extend previous work of Galleas and the author to elliptic SOS models. We demonstrate that the dynamical reflection algebra can be exploited to obtain a functional equation characterizing the partition function of an elliptic SOS model with domain-wall boundaries and one reflecting end. Special attention is paid to the structure of the functional equation. Through this approach we find a novel multiple-integral formula for that partition function.

  1. Factors limiting SOS expression in log-phase cells of Escherichia coli.

    Science.gov (United States)

    Massoni, Shawn C; Leeson, Michael C; Long, Jarukit Edward; Gemme, Kristin; Mui, Alice; Sandler, Steven J

    2012-10-01

    In Escherichia coli, RecA-single-stranded DNA (RecA-ssDNA) filaments catalyze DNA repair, recombination, and induction of the SOS response. It has been shown that, while many (15 to 25%) log-phase cells have RecA filaments, few (about 1%) are induced for SOS. It is hypothesized that RecA's ability to induce SOS expression in log-phase cells is repressed because of the potentially detrimental effects of SOS mutagenesis. To test this, mutations were sought to produce a population where the number of cells with SOS expression more closely equaled the number of RecA filaments. Here, it is shown that deleting radA (important for resolution of recombination structures) and increasing recA transcription 2- to 3-fold with a recAo1403 operator mutation act independently to minimally satisfy this condition. This allows 24% of mutant cells to have elevated levels of SOS expression, a percentage similar to that of cells with RecA-green fluorescent protein (RecA-GFP) foci. In an xthA (exonuclease III gene) mutant where there are 3-fold more RecA loading events, recX (a destabilizer of RecA filaments) must be additionally deleted to achieve a population of cells where the percentage having elevated SOS expression (91%) nearly equals the percentage with at least one RecA-GFP focus (83%). It is proposed that, in the xthA mutant, there are three independent mechanisms that repress SOS expression in log-phase cells. These are the rapid processing of RecA filaments by RadA, maintaining the concentration of RecA below a critical level, and the destabilizing of RecA filaments by RecX. Only the first two mechanisms operate independently in a wild-type cell.

  2. Escherichia coli DinB inhibits replication fork progression without significantly inducing the SOS response.

    Science.gov (United States)

    Mori, Tetsuya; Nakamura, Tatsuro; Okazaki, Naoto; Furukohri, Asako; Maki, Hisaji; Akiyama, Masahiro Tatsumi

    2012-01-01

    The SOS response is readily triggered by replication fork stalling caused by DNA damage or a dysfunctional replicative apparatus in Escherichia coli cells. E. coli dinB encodes DinB DNA polymerase and its expression is upregulated during the SOS response. DinB catalyzes translesion DNA synthesis in place of a replicative DNA polymerase III that is stalled at a DNA lesion. We showed previously that DNA replication was suppressed without exogenous DNA damage in cells overproducing DinB. In this report, we confirm that this was due to a dose-dependent inhibition of ongoing replication forks by DinB. Interestingly, the DinB-overproducing cells did not significantly induce the SOS response even though DNA replication was perturbed. RecA protein is activated by forming a nucleoprotein filament with single-stranded DNA, which leads to the onset of the SOS response. In the DinB-overproducing cells, RecA was not activated to induce the SOS response. However, the SOS response was observed after heat-inducible activation in strain recA441 (encoding a temperature-sensitive RecA) and after replication blockage in strain dnaE486 (encoding a temperature-sensitive catalytic subunit of the replicative DNA polymerase III) at a non-permissive temperature when DinB was overproduced in these cells. Furthermore, since catalytically inactive DinB could avoid the SOS response to a DinB-promoted fork block, it is unlikely that overproduced DinB takes control of primer extension and thus limits single-stranded DNA. These observations suggest that DinB possesses a feature that suppresses DNA replication but does not abolish the cell's capacity to induce the SOS response. We conclude that DinB impedes replication fork progression in a way that does not activate RecA, in contrast to obstructive DNA lesions and dysfunctional replication machinery.

  3. [SOS response of DNA repair and genetic cell instability under hypoxic conditions].

    Science.gov (United States)

    Vasil'eva, S V; Strel'tsova, D A

    2011-01-01

    The SOS DNA repair pathway is induced in E. coli as a multifunctional cell response to a wide variety of signals: UV, X or gamma-irradiation, mitomycin C or nalidixic acid treatment, thymine starvation, etc. Triggering of the system can be used as a general and early sign of DNA damage. Additionally, the SOS-response is known to be an "error-prone" DNA repair pathway and one of the sources of genetic instability. Hypoxic conditions are established to be the major factor of genetic instability as well. In this paper we for the first time studied the SOS DNA repair response under hypoxic conditions induced by the well known aerobic SOS-inducers. The SOS DNA repair response was examined as a reaction of E. coli PQ37 [sfiA::lacZ] cells to UVC, NO-donating agents and 4NQO. Here we provide evidence that those agents were able to induce the SOS DNA repair response in E. coli at anaerobic growth conditions. The process does not depend on the transcriptional activity of the universal protein of E. col anaerobic growth Fnr [4Fe-4S]2+ or can not be referred to as an indicator of genetic instability in hypoxic conditions.

  4. SOS reaction kinetics of bacterial cells induced by ultraviolet radiation and α particles

    International Nuclear Information System (INIS)

    Bonev, M.; Kolev, S.

    2000-01-01

    It is the purpose of the work to apply the SOS lux test for detecting α particles, as well as to study the SOS system kinetics. Two strains with plasmid pPLS-1 are used: wild type C600 lux and its isogen lysogen with α prophage one. Irradiation is done on dacron nuclear filters. The source of α particles is Am 241 with power 5 Gy/min, and the ultraviolet source - a lamp emitting rays with wave length 254 nm. The light yield is measured by installations made up of scintilometer VA-S-968, High-voltage electric power, and one channel analyzer Strahlugsmessgerat 20046. The SOS lux text is based on the recombinant plasmid pPLS-1 which is a derivative of pBR322 where the lux gene is set under the control of an SOS promoter. E coly recA + strains containing the construction produce considerable amount of photons in the visible zone following treatment with agents damaging the DNA of cells. The kinetic curves of SOS response are obtained after irradiation with α particles and UV rays. DNA damaging agents cause an increase in the initial SOS response rate in the range od smaller doses, and a decrease reaching to block of the one in the high doses range. The light yield of lysogenic cells is lower. As compared to nonelysogene ones. DNA damage caused by α particles are more difficult to repair as compared to pyrimidine dimers. (author)

  5. Stent-over-sponge (SOS): a novel technique complementing endosponge therapy for foregut leaks and perforations.

    Science.gov (United States)

    Valli, Piero V; Mertens, Joachim C; Kröger, Arne; Gubler, Christoph; Gutschow, Christian; Schneider, Paul M; Bauerfeind, Peter

    2018-02-01

     Endoluminal vacuum therapy (EVT) has evolved as a promising option for endoscopic treatment of foregut wall injuries in addition to the classic closure techniques using clips or stents. To improve vacuum force and maintain esophageal passage, we combined endosponge treatment with a partially covered self-expandable metal stent (stent-over-sponge; SOS).  Twelve patients with infected upper gastrointestinal wall defects were treated with the SOS technique.  Indications for SOS were anastomotic leakage after surgery (n = 11) and chronic foregut fistula (n = 1). SOS treatment was used as a first-line treatment in seven patients with a success rate of 71.4 % (5/7) and as a second-line treatment after failed previous EVT treatment in five patients (success rate 80 %; 4/5). Overall, SOS treatment was successful in 75 % of patients (9/12). No severe adverse events occurred. CONCLUSION : SOS is an effective method to treat severely infected foregut wall defects in patients where EVT has failed, and also as a first-line treatment. Comparative prospective studies are needed to confirm our preliminary results. © Georg Thieme Verlag KG Stuttgart · New York.

  6. Sinusoidal obstruction syndrome (SOS): A light and electron microscopy study in human liver.

    Science.gov (United States)

    Vreuls, C P H; Driessen, A; Olde Damink, S W M; Koek, G H; Duimel, H; van den Broek, M A J; Dejong, C H C; Braet, F; Wisse, E

    2016-05-01

    Oxaliplatin is an important chemotherapeutic agent, used in the treatment of hepatic colorectal metastases, and known to induce the sinusoidal obstruction syndrome (SOS). Pathophysiological knowledge concerning SOS is based on a rat model. Therefore, the aim was to perform a comprehensive study of the features of human SOS, using both light microscopy (LM) and electron microscopy (EM). Included were all patients of whom wedge liver biopsies were collected during a partial hepatectomy for colorectal liver metastases, in a 4-year period. The wedge biopsy were perfusion fixated and processed for LM and EM. The SOS lesions were selected by LM and details were studied using EM. Material was available of 30 patients, of whom 28 patients received neo-adjuvant oxaliplatin. Eighteen (64%) of the 28 patients showed SOS lesions, based on microscopy. The lesions consisted of sinusoidal endothelial cell detachment from the space of Disse on EM. In the enlarged space of Disse a variable amount of erythrocytes were located. Sinusoidal endothelial cell detachment was present in human SOS, accompanied by enlargement of the space of Disse and erythrocytes in this area. These findings, originally described in a rat model, were now for the first time confirmed in human livers under clinically relevant settings. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Induction of the SOS system in Escherichia coli after UVA (320 - 400 nm) irradiation

    International Nuclear Information System (INIS)

    Batbyamba, G.; Drasil, V.

    1988-01-01

    Induction of the SOS repair system in E. coli caused by broad-band (320 - 400 nm) UVA radiation and an oxygen effect in this induction were studied using the sfiA::lacZ operon fusion. Moreover, an oxygen effect on the broad-band UVA radiation-induced cell killing was studied. The experiments indicate that: (1) Broad-band UVA light can produce lethal damage to cells as well as DNA damage able to generate an SOS-inducing signal. This damage is O 2 -dependent to a significant extent: SOSIP (O 2 )/ SOSIP (Ar) = 1.61 and OER = 1.96; (2) After UVA irradiation the SOS induction factor increases monotonously in the time interval longer than 4 h indicating that the SOS-inducing DNA damage caused by UVA irradiation has a 'long-lived' character; (3) Oxic and hypoxic incubation following UVA irradiation carried out under aerobic and anaerobic conditions resulted in a strong oxygen effect: SOSIP(O 2 )/SOSIP(Ar) ∼ 5. On the basis of these results and literary data it was concluded that one of the main toxic photoproducts formed as a result of UVA irradiation of the cells in a culture medium might be hydrogen peroxide (H 2 O 2 ). H 2 O 2 decays gradually during post-irradiation incubation and yields reactive radical species, mainly OH radical, that result in a formation of SOS-inducing DNA damages and contribute to cell lethality, and prolonged SOS induction. (author)

  8. Ultrassonografia quantitativa do fígado em gatos com tirotoxicose induzida

    Directory of Open Access Journals (Sweden)

    Warley Gomes dos Santos

    2009-12-01

    Full Text Available Este estudo avaliou ultrassonograficamente o fígado de gatos submetidos à tirotoxicose induzida pela técnica de histograma dos níveis de cinza com o objetivo de melhor avaliar possíveis alterações hepáticas decorrentes do excesso de hormônios tiroidianos. Para isto, foram utilizados nove gatos hígidos, adulto-jovens, que receberam diariamente 150µg/kg de levotiroxina sódica, por 60 dias. O histograma hepático não demonstrou diferença estatística ao nível de 5% de significância entre os momentos avaliados; entretanto, as variações dos valores das variáveis estudadas sugeriram a promoção de aumento da heterogeneidade e diminuição da ecogenicidade do parênquima hepático quando comparado ao momento inicial. A avaliação citopatológica e histopatológica revelou quadro de hepatite tirotóxica em todos os animais.

  9. Glyceride structure and sterol composition of SOS-7 halophyte oil

    Directory of Open Access Journals (Sweden)

    El-Shami, S. M.

    1991-06-01

    Full Text Available Glyceride structure of SOS-7 halophyte oil was studied using the lipase hydrolysis technique. This halophyte sample was obtained from 1988 harvest planted in Ghardaka, on the border of the Red Sea, Egypt. The oilseed was ground and extracted for its oil using commercial hexane in Soxhlet extractor. The unsaturated fatty acids were found centralized in the 2-position of triglycerides, whereas oleic and linolenic acids showed more preference for this position. It was found that P3 was the major component of GS3, whereas P2L and PStL; PL2, POL and StL2 are predominating among GS2U and GSU3 respectively. L3 manifested itself as the principal constituent of GU3 type. Sterol composition of the halophyte oil was determined by GLC as TMS derivative. It was found that the oil contains campsterol, β-sitosterol, stigmasterol and 7-stigmasterol of which 7-stigmasterol is the major sterol and constitute 52.4%.

    Se ha estudiado usando la técnica de hidrólisis mediante lipasa la estructura glicerídica de aceite de halofito SOS-7. Esta muestra de halofito fue obtenida a partir de una cosecha de 1988 plantada en Ghardaka, en la orilla del Mar Rojo, Egipto. Para la extracción del aceite de la semilla molida se utilizó hexano comercial en extractor Soxhlet. Los ácidos grasos insaturados se encontraron centralizados en la posición 2 de los triglicéridos, siendo los ácidos oleico y linolénico los que mostraron mayor preferencia por esta posición. Se encontró que P3 fue el componente mayoritario de GS3, mientras que P2L y PStL; PL2 POL y StL2 son los predominantes para GS2U y GSU3 respectivamente. L3 se manifestó como el principal constituyente de los GU3. La composición esterólica del aceite de halofito se determinó por GLC como derivados del

  10. Freshly isolated hepatocyte transplantation in acetaminophen-induced hepatotoxicity model in rats Transplante de hepatócitos recém-isolados em um modelo de hepatotoxicidade induzida por acetaminofeno em ratos

    Directory of Open Access Journals (Sweden)

    Daniela Rodrigues

    2012-12-01

    Full Text Available CONTEXT: Hepatocyte transplantation is an attractive therapeutic modality for liver disease as an alternative for orthotopic liver transplantation. OBJECTIVE: The aim of the current study was to investigate the feasibility of freshly isolated rat hepatocyte transplantation in acetaminophen-induced hepatotoxicity model. METHODS: Hepatocytes were isolated from male Wistar rats and transplanted 24 hours after acetaminophen administration in female recipients. Female rats received either 1x10(7 hepatocytes or phosphate buffered saline through the portal vein or into the spleen and were sacrificed after 48 hours. RESULTS: Alanine aminotransferase levels measured within the experiment did not differ between groups at any time point. Molecular analysis and histology showed presence of hepatocytes in liver of transplanted animals injected either through portal vein or spleen. CONCLUSION: These data demonstrate the feasibility and efficacy of hepatocyte transplantation in the liver or spleen in a mild acetaminophen-induced hepatotoxicity model.CONTEXTO: O transplante de hepatócitos é uma modalidade terapêutica atrativa para doenças hepáticas como alternativa ao transplante hepático ortotópico. OBJETIVO: Investigar a factibilidade do uso de hepatócitos frescos isolados de ratos em um modelo de hepatotoxicidade induzida por paracetamol. MÉTODOS: Hepatócitos foram isolados de ratos Wistar machos e transplantados 24 horas após a administração de paracetamol em receptores fêmeas. As ratas receberam 1x10(7 hepatócitos ou tampão salina fosfato pela veia porta ou no baço e foram sacrificadas após 48 horas. RESULTADOS: Os níveis de alanina aminotransferase medidos durante o experimento não diferiram entre os grupos em nenhum momento. Análises moleculares e histológicas demonstraram a presença de hepatócitos no fígado dos animais transplantados pelo baço ou pela veia porta. CONCLUSÃO: Os dados indicam a factibilidade e eficácia do

  11. Expansion of the SOS regulon of Vibrio cholerae through extensive transcriptome analysis and experimental validation.

    Science.gov (United States)

    Krin, Evelyne; Pierlé, Sebastian Aguilar; Sismeiro, Odile; Jagla, Bernd; Dillies, Marie-Agnès; Varet, Hugo; Irazoki, Oihane; Campoy, Susana; Rouy, Zoé; Cruveiller, Stéphane; Médigue, Claudine; Coppée, Jean-Yves; Mazel, Didier

    2018-05-21

    The SOS response is an almost ubiquitous response of cells to genotoxic stresses. The full complement of genes in the SOS regulon for Vibrio species has only been addressed through bioinformatic analyses predicting LexA binding box consensus and in vitro validation. Here, we perform whole transcriptome sequencing from Vibrio cholerae treated with mitomycin C as an SOS inducer to characterize the SOS regulon and other pathways affected by this treatment. Comprehensive transcriptional profiling allowed us to define the full landscape of promoters and transcripts active in V. cholerae. We performed extensive transcription start site (TSS) mapping as well as detection/quantification of the coding and non-coding RNA (ncRNA) repertoire in strain N16961. To improve TSS detection, we developed a new technique to treat RNA extracted from cells grown in various conditions. This allowed for identification of 3078 TSSs with an average 5'UTR of 116 nucleotides, and peak distribution between 16 and 64 nucleotides; as well as 629 ncRNAs. Mitomycin C treatment induced transcription of 737 genes and 28 ncRNAs at least 2 fold, while it repressed 231 genes and 17 ncRNAs. Data analysis revealed that in addition to the core genes known to integrate the SOS regulon, several metabolic pathways were induced. This study allowed for expansion of the Vibrio SOS regulon, as twelve genes (ubiEJB, tatABC, smpA, cep, VC0091, VC1190, VC1369-1370) were found to be co-induced with their adjacent canonical SOS regulon gene(s), through transcriptional read-through. Characterization of UV and mitomycin C susceptibility for mutants of these newly identified SOS regulon genes and other highly induced genes and ncRNAs confirmed their role in DNA damage rescue and protection. We show that genotoxic stress induces a pervasive transcriptional response, affecting almost 20% of the V. cholerae genes. We also demonstrate that the SOS regulon is larger than previously known, and its syntenic organization is

  12. Há uma associação entre anti-inflamatórios não-esteroides e nefropatia induzida por contraste?

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    Luciano Passamani Diogo

    Full Text Available FUNDAMENTO: A associação entre o uso de anti-inflamatórios não-esteroides (AINEs e insuficiência renal aguda ou crônica é bem documentada, mas evidências sobre a associação entre AINEs e nefropatia induzida por contraste (NIC não são encontradas na literatura. OBJETIVO: Avaliar uma possível associação entre AINEs e NIC. MÉTODOS: Em um estudo de coorte, através da entrevista clínica de pacientes que foram submetidos à cateterização cardíaca, analisamos o uso de AINEs e sua associação com desenvolvimento de NIC, através da alteração dos níveis de creatinina sérica ou taxa de filtração glomerular em 48 ou 72 horas. RESULTADOS: No período de julho de 2005 a julho de 2006, 236 pacientes foram incluídos no estudo, dos quais 29 foram posteriormente excluídos. A incidência de NIC foi 10,37% (20 de 207 e 42% dos pacientes estavam recebendo AINEs até o momento da avaliação. Não houve associação entre o uso de AINEs e o desenvolvimento de NIC com OR de 1,293; IC95% (0,46-4,2. O estudo detectou fatores de risco conhecidos para o desenvolvimento de NIC, tais como diabete, com OR de 2,77; IC95% (1,05-7,47 e insuficiência renal crônica com OR de 3,48; IC95% (1,1-11,07 e também sugeriu uma ação protetora da hidratação com solução salina com OR de 0,166; IC95% (0,03-0,92. CONCLUSÃO: Com base nos dados obtidos, concluímos que não houve associação entre NIC e uso prévio de AINEs, pelo menos com um OR > 2,85, o qual nossa amostra detectou.

  13. SOS switch system (SSS) in the radiation treatment room

    International Nuclear Information System (INIS)

    Komiyama, Takafumi; Motoyama, Tsuyoshi; Nakamura, Koji; Onishi, Hiroshi; Araya, Masayuki; Sano, Naoki

    2009-01-01

    We applied patient's self-breath hold irradiation system to a device to declare the patient's intentions (SOS switch system: SSS) in the radiation room and examined a utility for problem recognition and improvement of risk management during radiation therapy by induction of SSS. Between May 2005 and October 2006, we used SSS with 65 patients. The study involved 32 men and 33 women with a median age of 65 (range, 26-88) years. The reason for using SSS was as a shell in 57, a history of laryngectomy in 2, a cough in 6, convulsions in 1, and anxiety in 3. The treatment with SSS was performed 1,120 times. The hand switch was pushed 11 times. The reasons the switch was pushed were for nausea, aspiration, pain, and cough one time each. For the others, the reasons were unclear, and it was thought due to the clouding of consciousness from brain metastases. No problems were observed with the use of SSS. SSS was a useful device for improvement of risk management during the radiation therapy. (author)

  14. SOS score: an optimized score to screen acute stroke patients for obstructive sleep apnea.

    Science.gov (United States)

    Camilo, Millene R; Sander, Heidi H; Eckeli, Alan L; Fernandes, Regina M F; Dos Santos-Pontelli, Taiza E G; Leite, Joao P; Pontes-Neto, Octavio M

    2014-09-01

    Obstructive sleep apnea (OSA) is frequent in acute stroke patients, and has been associated with higher mortality and worse prognosis. Polysomnography (PSG) is the gold standard diagnostic method for OSA, but it is impracticable as a routine for all acute stroke patients. We evaluated the accuracy of two OSA screening tools, the Berlin Questionnaire (BQ), and the Epworth Sleepiness Scale (ESS) when administered to relatives of acute stroke patients; we also compared these tools against a combined screening score (SOS score). Ischemic stroke patients were submitted to a full PSG at the first night after onset of symptoms. OSA severity was measured by apnea-hypopnea index (AHI). BQ and ESS were administered to relatives of stroke patients before the PSG and compared to SOS score for accuracy and C-statistics. We prospectively studied 39 patients. OSA (AHI ≥10/h) was present in 76.9%. The SOS score [area under the curve (AUC): 0.812; P = 0.005] and ESS (AUC: 0.789; P = 0.009) had good predictive value for OSA. The SOS score was the only tool with significant predictive value (AUC: 0.686; P = 0.048) for severe OSA (AHI ≥30/h), when compared to ESS (P = 0.119) and BQ (P = 0.191). The threshold of SOS ≤10 showed high sensitivity (90%) and negative predictive value (96.2%) for OSA; SOS ≥20 showed high specificity (100%) and positive predictive value (92.5%) for severe OSA. The SOS score administered to relatives of stroke patients is a useful tool to screen for OSA and may decrease the need for PSG in acute stroke setting. Copyright © 2014 Elsevier B.V. All rights reserved.

  15. Survival and SOS response induction in ultraviolet B irradiated Escherichia coli cells with defective repair mechanisms.

    Science.gov (United States)

    Prada Medina, Cesar Augusto; Aristizabal Tessmer, Elke Tatjana; Quintero Ruiz, Nathalia; Serment-Guerrero, Jorge; Fuentes, Jorge Luis

    2016-06-01

    Purpose In this paper, the contribution of different genes involved in DNA repair for both survival and SOS induction in Escherichia coli mutants exposed to ultraviolet B radiation (UVB, [wavelength range 280-315 nm]) was evaluated. Materials and methods E. coli strains defective in uvrA, oxyR, recO, recN, recJ, exoX, recB, recD or xonA genes were used to determine cell survival. All strains also had the genetic sulA::lacZ fusion, which allowed for the quantification of SOS induction through the SOS Chromotest. Results Five gene products were particularly important for survival, as follows: UvrA > RecB > RecO > RecJ > XonA. Strains defective in uvrA and recJ genes showed elevated SOS induction compared with the wild type, which remained stable for up to 240 min after UVB-irradiation. In addition, E. coli strains carrying the recO or recN mutation showed no SOS induction. Conclusions The nucleotide excision and DNA recombination pathways were equally used to repair UVB-induced DNA damage in E. coli cells. The sulA gene was not turned off in strains defective in UvrA and RecJ. RecO protein was essential for processing DNA damage prior to SOS induction. In this study, the roles of DNA repair proteins and their contributions to the mechanisms that induce SOS genes in E. coli are proposed.

  16. The SOS response increases bacterial fitness, but not evolvability, under a sublethal dose of antibiotic.

    Science.gov (United States)

    Torres-Barceló, Clara; Kojadinovic, Mila; Moxon, Richard; MacLean, R Craig

    2015-10-07

    Exposure to antibiotics induces the expression of mutagenic bacterial stress-response pathways, but the evolutionary benefits of these responses remain unclear. One possibility is that stress-response pathways provide a short-term advantage by protecting bacteria against the toxic effects of antibiotics. Second, it is possible that stress-induced mutagenesis provides a long-term advantage by accelerating the evolution of resistance. Here, we directly measure the contribution of the Pseudomonas aeruginosa SOS pathway to bacterial fitness and evolvability in the presence of sublethal doses of ciprofloxacin. Using short-term competition experiments, we demonstrate that the SOS pathway increases competitive fitness in the presence of ciprofloxacin. Continued exposure to ciprofloxacin results in the rapid evolution of increased fitness and antibiotic resistance, but we find no evidence that SOS-induced mutagenesis accelerates the rate of adaptation to ciprofloxacin during a 200 generation selection experiment. Intriguingly, we find that the expression of the SOS pathway decreases during adaptation to ciprofloxacin, and this helps to explain why this pathway does not increase long-term evolvability. Furthermore, we argue that the SOS pathway fails to accelerate adaptation to ciprofloxacin because the modest increase in the mutation rate associated with SOS mutagenesis is offset by a decrease in the effective strength of selection for increased resistance at a population level. Our findings suggest that the primary evolutionary benefit of the SOS response is to increase bacterial competitive ability, and that stress-induced mutagenesis is an unwanted side effect, and not a selected attribute, of this pathway. © 2015 The Authors.

  17. Rare variants in SOS2 and LZTR1 are associated with Noonan syndrome.

    Science.gov (United States)

    Yamamoto, Guilherme Lopes; Aguena, Meire; Gos, Monika; Hung, Christina; Pilch, Jacek; Fahiminiya, Somayyeh; Abramowicz, Anna; Cristian, Ingrid; Buscarilli, Michelle; Naslavsky, Michel Satya; Malaquias, Alexsandra C; Zatz, Mayana; Bodamer, Olaf; Majewski, Jacek; Jorge, Alexander A L; Pereira, Alexandre C; Kim, Chong Ae; Passos-Bueno, Maria Rita; Bertola, Débora Romeo

    2015-06-01

    Noonan syndrome is an autosomal dominant, multisystemic disorder caused by dysregulation of the RAS/mitogen activated protein kinase (MAPK) pathway. Heterozygous, pathogenic variants in 11 known genes account for approximately 80% of cases. The identification of novel genes associated with Noonan syndrome has become increasingly challenging, since they might be responsible for very small fractions of the cases. A cohort of 50 Brazilian probands negative for pathogenic variants in the known genes associated with Noonan syndrome was tested through whole-exome sequencing along with the relatives in the familial cases. Families from the USA and Poland with mutations in the newly identified genes were included subsequently. We identified rare, segregating or de novo missense variants in SOS2 and LZTR1 in 4% and 8%, respectively, of the 50 Brazilian probands. SOS2 and LZTR1 variants were also found to segregate in one American and one Polish family. Notably, SOS2 variants were identified in patients with marked ectodermal involvement, similar to patients with SOS1 mutations. We identified two novel genes, SOS2 and LZTR1, associated with Noonan syndrome, thereby expanding the molecular spectrum of RASopathies. Mutations in these genes are responsible for approximately 3% of all patients with Noonan syndrome. While SOS2 is a natural candidate, because of its homology with SOS1, the functional role of LZTR1 in the RAS/MAPK pathway is not known, and it could not have been identified without the large pedigrees. Additional functional studies are needed to elucidate the role of LZTR1 in RAS/MAPK signalling and in the pathogenesis of Noonan syndrome. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

  18. Eletroacupuntura para tratamento de hipotensão induzida por isofluorano em cavalos

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    E.C. Franco

    2014-04-01

    Full Text Available Objetivou-se avaliar a eficiência do tratamento da hipotensão arterial com eletroacupuntura comparativamente à dobutamina em equinos. Foram avaliados seis cavalos adultos, saudáveis, mantidos em anestesia inalatória, com isofluorano, em ventilação mecânica. Após a estabilização da anestesia, foi induzida hipotensão arterial, através do incremento da concentração do isofluorano, iniciando-se um dos tratamentos: DOB: dobutamina (1,5µg kg-1 min-1, infusão contínua intravenosa; EA: estímulo elétrico no acuponto pericárdio 6 (PC6, bilateralmente; SHAM: estímulo elétrico em ponto falso de acupuntura. Foram mensurados: frequência cardíaca (FC, pressão arterial média (PAM, temperatura retal (T, concentração final expirada de isofluorano (ETiso, variáveis hemogasométricas, concentração sérica de aspartato aminotransferase (AST e creatina fosfoquinase (CK, tempo e qualidade da recuperação pós-anestésica. Houve incremento na PAM de 50%, 36,6% e 7,5% nos tratamentos DOB, EA e SHAM, respectivamente. Não houve diferença entre os grupos nas variáveis hemogasométricas, FC, T, ETiso, CK, AST, tempo e qualidade de recuperação pós-anestésica. Conclui-se que o tratamento com dobutamina foi mais efetivo para o tratamento da hipotensão em cavalos sob anestesia inalatória quando comparado ao estímulo elétrico do acuponto PC6 ou ponto falso de acupuntura.

  19. Crises parciais motoras induzidas por movimentos em pacientes diabéticos

    Directory of Open Access Journals (Sweden)

    Glória M. A. S. Tedrus

    1991-12-01

    Full Text Available Levando em conta o número restrito de casos em âmbito internacional e a ausência de publicações na literatura nacional, os autores apresentam três casos de crises motoras focais induzidas por movimentos em pacientes diabéticos com mais de 50 anos de idade. As crises eram numerosas, sendo também desencadeadas ao serem assumidas determinadas posturas. Em dois casos havia hiperglicemia não cetótica. Todos os pacientes apresentavam hemiparesia e, em dois, tal déficit desapareceu após o cotnrole das crises. No outro paciente a hemiparesia era decorrente de acidente vascular cerebral isquêmico instalado 6 meses antes. Síndrome parietal transitória foi observada nos três pacientes. O EEG crítico mostrou, em dois pacientes, descargas na região parieto-occipital e, em um, na região temporal média contralateral. As crises foram resistentes aos anticonvulsivantes, só havendo controle com o tratamento dos distúrbios metabólicos. São discutidos aspectos clínico-eletren-cefalográficos e etiopatogênicos. A pesquisa do caráter reflexo das crises e a dosagem da glicemia devem ser feitas em todos os pacientes idosos com crises motoras focais, face às implicações de ordem terapêutica e prognóstica.

  20. Adolescentes e crack: pelo caminho das pedras

    Directory of Open Access Journals (Sweden)

    Eduardo Tomm

    2013-12-01

    Full Text Available Nos últimos anos, a sociedade tem observado uma rápida expansão no uso de crack, especialmente por adolescentes e jovens. É evidente a necessidade de ouvir esses sujeitos de para melhor compreender sua situação. Para atender a essa necessidade, desenvolvemos uma pesquisa qualitativa descritivo-exploratória cujo foco foi um grupo terapêutico para adolescentes usuários de crack que ocorreu no Centro de Atenção Psicossocial Infanto-Juvenil (CAPSi em uma cidade do interior do Rio Grande do Sul, Brasil. Amparados na modalidade expost-facto, analisamos documentos produzidos nessa instituição. O resultado foi uma cartografia que acompanhou discursivamente os adolescentes pelo "caminho das pedras": os lugares, tratamentos, pessoas, ideias e momentos dos quais falam.

  1. Genetic requirements for high constitutive SOS expression in recA730 mutants of Escherichia coli.

    Science.gov (United States)

    Vlašić, Ignacija; Šimatović, Ana; Brčić-Kostić, Krunoslav

    2011-09-01

    The RecA protein in its functional state is in complex with single-stranded DNA, i.e., in the form of a RecA filament. In SOS induction, the RecA filament functions as a coprotease, enabling the autodigestion of the LexA repressor. The RecA filament can be formed by different mechanisms, but all of them require three enzymatic activities essential for the processing of DNA double-stranded ends. These are helicase, 5'-3' exonuclease, and RecA loading onto single-stranded DNA (ssDNA). In some mutants, the SOS response can be expressed constitutively during the process of normal DNA metabolism. The RecA730 mutant protein is able to form the RecA filament without the help of RecBCD and RecFOR mediators since it better competes with the single-strand binding (SSB) protein for ssDNA. As a consequence, the recA730 mutants show high constitutive SOS expression. In the study described in this paper, we studied the genetic requirements for constitutive SOS expression in recA730 mutants. Using a β-galactosidase assay, we showed that the constitutive SOS response in recA730 mutants exhibits different requirements in different backgrounds. In a wild-type background, the constitutive SOS response is partially dependent on RecBCD function. In a recB1080 background (the recB1080 mutation retains only helicase), constitutive SOS expression is partially dependent on RecBCD helicase function and is strongly dependent on RecJ nuclease. Finally, in a recB-null background, the constitutive SOS expression of the recA730 mutant is dependent on the RecJ nuclease. Our results emphasize the importance of the 5'-3' exonuclease for high constitutive SOS expression in recA730 mutants and show that RecBCD function can further enhance the excellent intrinsic abilities of the RecA730 protein in vivo. Copyright © 2011, American Society for Microbiology. All Rights Reserved.

  2. A metabonomic evaluation of the monocrotaline-induced sinusoidal obstruction syndrome (SOS) in rats

    International Nuclear Information System (INIS)

    Conotte, R.; Colet, J.-M.

    2014-01-01

    The main curative treatment of colorectal cancer remains the surgery. However, when metastases are suspected, surgery is followed by a preventive chemotherapy using oxaliplatin which, unfortunately, may cause liver sinusoidal obstruction syndrome (SOS). Such hepatic damage is barely detected during or after chemotherapy due to a lack of effective diagnostic procedures, but liver biopsy. The primary objective of the present study was to identify potential early diagnosis biomarkers of SOS using a metabonomic approach. SOS was induced in rats by monocrotaline, a prototypical toxic substance. 1 H NMR spectroscopy analysis of urine samples collected from rats treated with monocrotaline showed significant metabolic changes as compared to controls. During a first phase, cellular protective mechanisms such as an increased synthesis of GSH (reduced taurine) and the recruitment of cell osmolytes in the liver (betaine) were seen. In the second phase, the disturbance of the urea cycle (increased ornithine and urea reduction) leading to the depletion of NO, the alteration in the GSH synthesis (increased creatine and GSH precursors (glutamate, dimethylglycine and sarcosine)), and the liver necrosis (decrease taurine and increase creatine) all indicate the development of SOS. - Highlights: • Urine metabonomic profiles of SOS have been identified. • Urine osmoprotectants and anti-oxidants indicated an initial liver protection. • Liver necrosis was demonstrated by increased urine levels of taurine and creatine. • NO depletion was suggested by changes in ornithine and urea

  3. Evaluation of effects of busulfan and DMA on SOS in pediatric stem cell recipients.

    Science.gov (United States)

    Kerl, Kornelius; Diestelhorst, Christian; Bartelink, Imke; Boelens, Jaap; Trame, Mirjam N; Boos, Joachim; Hempel, Georg

    2014-02-01

    Busulfan (Bu) is a DNA-alkylating agent used for myeloablative conditioning in stem cell transplantation in children and adults. While the use of intravenous rather than oral administration of Bu has reduced inter-individual variability in plasma levels, toxicity still occurs frequently after hematopoietic stem cell transplantation (HSCT). Toxicity (especially hepatotoxic effects) of intravenous (IV) Bu may be related to both Bu and/or N,N-dimethylacetamide (DMA), the solvent of Bu. In this study, we assessed the relation between the exposure of Bu and DMA with regards to the clinical outcome in children from two cohorts. In a two-centre study Bu and DMA AUC (area under the curve) were correlated in pediatric stem cell recipients to the risk of developing SOS and to the clinical outcome. In patients receiving Bu four times per day Bu levels >1,500 µmol/L minute correlate to an increased risk of developing a SOS. In the collective cohort, summarizing data of all 53 patients of this study, neither high area under the curve (AUC) of Bu nor high AUC of DMA appears to be an independent risk factor for the development of SOS in children. In this study neither Bu nor DMA was observed as an independent risk factor for the development of SOS. To identify subgroups (e.g., infants), in which Bu or DMA might be risk factors for the induction of SOS, larger cohorts have to be evaluated. © 2013 Wiley Periodicals, Inc.

  4. Atomic orbital-based SOS-MP2 with tensor hypercontraction. II. Local tensor hypercontraction

    Science.gov (United States)

    Song, Chenchen; Martínez, Todd J.

    2017-01-01

    In the first paper of the series [Paper I, C. Song and T. J. Martinez, J. Chem. Phys. 144, 174111 (2016)], we showed how tensor-hypercontracted (THC) SOS-MP2 could be accelerated by exploiting sparsity in the atomic orbitals and using graphical processing units (GPUs). This reduced the formal scaling of the SOS-MP2 energy calculation to cubic with respect to system size. The computational bottleneck then becomes the THC metric matrix inversion, which scales cubically with a large prefactor. In this work, the local THC approximation is proposed to reduce the computational cost of inverting the THC metric matrix to linear scaling with respect to molecular size. By doing so, we have removed the primary bottleneck to THC-SOS-MP2 calculations on large molecules with O(1000) atoms. The errors introduced by the local THC approximation are less than 0.6 kcal/mol for molecules with up to 200 atoms and 3300 basis functions. Together with the graphical processing unit techniques and locality-exploiting approaches introduced in previous work, the scaled opposite spin MP2 (SOS-MP2) calculations exhibit O(N2.5) scaling in practice up to 10 000 basis functions. The new algorithms make it feasible to carry out SOS-MP2 calculations on small proteins like ubiquitin (1231 atoms/10 294 atomic basis functions) on a single node in less than a day.

  5. A metabonomic evaluation of the monocrotaline-induced sinusoidal obstruction syndrome (SOS) in rats

    Energy Technology Data Exchange (ETDEWEB)

    Conotte, R.; Colet, J.-M., E-mail: jean-marie.colet@umons.ac.be

    2014-04-15

    The main curative treatment of colorectal cancer remains the surgery. However, when metastases are suspected, surgery is followed by a preventive chemotherapy using oxaliplatin which, unfortunately, may cause liver sinusoidal obstruction syndrome (SOS). Such hepatic damage is barely detected during or after chemotherapy due to a lack of effective diagnostic procedures, but liver biopsy. The primary objective of the present study was to identify potential early diagnosis biomarkers of SOS using a metabonomic approach. SOS was induced in rats by monocrotaline, a prototypical toxic substance. {sup 1}H NMR spectroscopy analysis of urine samples collected from rats treated with monocrotaline showed significant metabolic changes as compared to controls. During a first phase, cellular protective mechanisms such as an increased synthesis of GSH (reduced taurine) and the recruitment of cell osmolytes in the liver (betaine) were seen. In the second phase, the disturbance of the urea cycle (increased ornithine and urea reduction) leading to the depletion of NO, the alteration in the GSH synthesis (increased creatine and GSH precursors (glutamate, dimethylglycine and sarcosine)), and the liver necrosis (decrease taurine and increase creatine) all indicate the development of SOS. - Highlights: • Urine metabonomic profiles of SOS have been identified. • Urine osmoprotectants and anti-oxidants indicated an initial liver protection. • Liver necrosis was demonstrated by increased urine levels of taurine and creatine. • NO depletion was suggested by changes in ornithine and urea.

  6. Approach for targeting Ras with small molecules that activate SOS-mediated nucleotide exchange.

    Science.gov (United States)

    Burns, Michael C; Sun, Qi; Daniels, R Nathan; Camper, DeMarco; Kennedy, J Phillip; Phan, Jason; Olejniczak, Edward T; Lee, Taekyu; Waterson, Alex G; Rossanese, Olivia W; Fesik, Stephen W

    2014-03-04

    Aberrant activation of the small GTPase Ras by oncogenic mutation or constitutively active upstream receptor tyrosine kinases results in the deregulation of cellular signals governing growth and survival in ∼30% of all human cancers. However, the discovery of potent inhibitors of Ras has been difficult to achieve. Here, we report the identification of small molecules that bind to a unique pocket on the Ras:Son of Sevenless (SOS):Ras complex, increase the rate of SOS-catalyzed nucleotide exchange in vitro, and modulate Ras signaling pathways in cells. X-ray crystallography of Ras:SOS:Ras in complex with these molecules reveals that the compounds bind in a hydrophobic pocket in the CDC25 domain of SOS adjacent to the Switch II region of Ras. The structure-activity relationships exhibited by these compounds can be rationalized on the basis of multiple X-ray cocrystal structures. Mutational analyses confirmed the functional relevance of this binding site and showed it to be essential for compound activity. These molecules increase Ras-GTP levels and disrupt MAPK and PI3K signaling in cells at low micromolar concentrations. These small molecules represent tools to study the acute activation of Ras and highlight a pocket on SOS that may be exploited to modulate Ras signaling.

  7. Viral and cellular SOS-regulated motor proteins: dsDNA translocation mechanisms with divergent functions.

    Science.gov (United States)

    Wolfe, Annie; Phipps, Kara; Weitao, Tao

    2014-01-01

    DNA damage attacks on bacterial cells have been known to activate the SOS response, a transcriptional response affecting chromosome replication, DNA recombination and repair, cell division and prophage induction. All these functions require double-stranded (ds) DNA translocation by ASCE hexameric motors. This review seeks to delineate the structural and functional characteristics of the SOS response and the SOS-regulated DNA translocases FtsK and RuvB with the phi29 bacteriophage packaging motor gp16 ATPase as a prototype to study bacterial motors. While gp16 ATPase, cellular FtsK and RuvB are similarly comprised of hexameric rings encircling dsDNA and functioning as ATP-driven DNA translocases, they utilize different mechanisms to accomplish separate functions, suggesting a convergent evolution of these motors. The gp16 ATPase and FtsK use a novel revolution mechanism, generating a power stroke between subunits through an entropy-DNA affinity switch and pushing dsDNA inward without rotation of DNA and the motor, whereas RuvB seems to employ a rotation mechanism that remains to be further characterized. While FtsK and RuvB perform essential tasks during the SOS response, their roles may be far more significant as SOS response is involved in antibiotic-inducible bacterial vesiculation and biofilm formation as well as the perspective of the bacteria-cancer evolutionary interaction.

  8. Hydrothermal Carbonization of Spent Osmotic Solution (SOS Generated from Osmotic Dehydration of Blueberries

    Directory of Open Access Journals (Sweden)

    Kaushlendra Singh

    2014-09-01

    Full Text Available Hydrothermal carbonization of spent osmotic solution (SOS, a waste generated from osmotic dehydration of fruits, has the potential of transformation into hydrochars, a value-added product, while reducing cost and overall greenhouse gas emissions associated with waste disposal. Osmotic solution (OS and spent osmotic solution (SOS generated from the osmotic dehydration of blueberries were compared for their thermo-chemical decomposition behavior and hydrothermal carbonization. OS and SOS samples were characterized for total solids, elemental composition, and thermo-gravimetric analysis (TGA. In addition, hydrothermal carbonization was performed at 250 °C and for 30 min to produce hydrochars. The hydrochars were characterized for elemental composition, Brunauer-Emmett-Teller (BET surface area, particle shape and surface morphology. TGA results show that the SOS sample loses more weight in the lower temperature range than the OS sample. Both samples produced, approximately, 40%–42% (wet-feed basis hydrochar during hydrothermal carbonization but with different properties. The OS sample produced hydrochar, which had spherical particles of 1.79 ± 1.30 μm diameter with a very smooth surface. In contrast, the SOS sample produced hydrochar with no definite particle shape but with a raspberry-like surface.

  9. Phenomenology of an inducible mutagenic DNA repair pathway in Escherichia coli: SOS repair hypothesis

    International Nuclear Information System (INIS)

    Radman, M.

    1974-01-01

    A hypothesis is proposed according to which E. coli possesses an inducible DNA repair system. This hypothetical repair, which we call SOS repair, is manifested only following damage to DNA, and requires de novo protein synthesis. SOS repair in E. coli requires some known genetic elements: recA + , lex + and probably zab + . Mutagenesis by ultraviolet light is observed only under conditions of functional SOS repair: we therefore suspect that this is a mutation-prone repair. A number of phenomena and experiments is reviewed which at this point can best be interpreted in terms of an inducible mutagenic DNA repair system. Two recently discovered phenomena support the proposed hypothesis: existence of a mutant (tif) which, after a shift to elevated temperature, mimicks the effect of uv irradiation in regard to repair of phage lambda and uv mutagenesis, apparent activation of SOS repair by introduction into the recipient cell of damaged plasmid or Hfr DNA. Several specific predictions based on SOS repair hypothesis are presented in order to stimulate further experimental tests. (U.S.)

  10. Sos - response induction by gamma radiation in Escherichia coli strains with different repair capacities

    International Nuclear Information System (INIS)

    Serment Guerrero, J.H.

    1992-01-01

    The Sos - response in Escherichia coli is formed by several genes involved in mechanisms of tolerance and/or repair, and only activates when a DNA - damage appears. It is controlled by recA and lexA genes. In normal circumstances, LexA protein is linked in every Sos operators, blocking the transcription. When a DNA damage occurs, a Sos signal is generated, Rec A protein changes its normal functions, starts acting as a protease and cleaves Lex A, allowing the transcription of all Sos genes. This response can be quantified by means of Sos Chromo test, performed by Quillardet and Ofnung (1985). In using the Chromo test, it has been observed that the DNA damage made by gamma radiation in Escherichia coli depends on both the doses and the doses rate. It has been shown that the exposure of Escherichia coli PQ37 strain (uvrA) to low doses at low dose rate appears to retard the response, suggesting the action of a repair mechanism. (Brena 1990). In this work, we compare the response in Escherichia coli strains deficient in different mechanisms of repair and/or tolerance. It is observed the importance of rec N gene in the repair of DNA damage produced by gamma radiation. (Author)

  11. Effect of the SOS response on the mean fitness of unicellular populations: a quasispecies approach.

    Science.gov (United States)

    Kama, Amit; Tannenbaum, Emmanuel

    2010-11-30

    The goal of this paper is to develop a mathematical model that analyzes the selective advantage of the SOS response in unicellular organisms. To this end, this paper develops a quasispecies model that incorporates the SOS response. We consider a unicellular, asexually replicating population of organisms, whose genomes consist of a single, double-stranded DNA molecule, i.e. one chromosome. We assume that repair of post-replication mismatched base-pairs occurs with probability , and that the SOS response is triggered when the total number of mismatched base-pairs is at least . We further assume that the per-mismatch SOS elimination rate is characterized by a first-order rate constant . For a single fitness peak landscape where the master genome can sustain up to mismatches and remain viable, this model is analytically solvable in the limit of infinite sequence length. The results, which are confirmed by stochastic simulations, indicate that the SOS response does indeed confer a fitness advantage to a population, provided that it is only activated when DNA damage is so extensive that a cell will die if it does not attempt to repair its DNA.

  12. Differential requirements of two recA mutants for constitutive SOS expression in Escherichia coli K-12.

    Directory of Open Access Journals (Sweden)

    Jarukit Edward Long

    Full Text Available Repairing DNA damage begins with its detection and is often followed by elicitation of a cellular response. In E. coli, RecA polymerizes on ssDNA produced after DNA damage and induces the SOS Response. The RecA-DNA filament is an allosteric effector of LexA auto-proteolysis. LexA is the repressor of the SOS Response. Not all RecA-DNA filaments, however, lead to an SOS Response. Certain recA mutants express the SOS Response (recA(C in the absence of external DNA damage in log phase cells.Genetic analysis of two recA(C mutants was used to determine the mechanism of constitutive SOS (SOS(C expression in a population of log phase cells using fluorescence of single cells carrying an SOS reporter system (sulAp-gfp. SOS(C expression in recA4142 mutants was dependent on its initial level of transcription, recBCD, recFOR, recX, dinI, xthA and the type of medium in which the cells were grown. SOS(C expression in recA730 mutants was affected by none of the mutations or conditions tested above.It is concluded that not all recA(C alleles cause SOS(C expression by the same mechanism. It is hypothesized that RecA4142 is loaded on to a double-strand end of DNA and that the RecA filament is stabilized by the presence of DinI and destabilized by RecX. RecFOR regulate the activity of RecX to destabilize the RecA filament. RecA730 causes SOS(C expression by binding to ssDNA in a mechanism yet to be determined.

  13. Estimulação elétrica neuromuscular em cães com atrofia muscular induzida Neuromuscular electric stimulation in dogs with induced muscle atrophy

    Directory of Open Access Journals (Sweden)

    C. Pelizzari

    2008-02-01

    Full Text Available Empregou-se a estimulação elétrica neuromuscular (EENM de baixa freqüência no músculo quadríceps femoral de cães com atrofia induzida e avaliou-se a ocorrência de ganho de massa nessa musculatura. Foram utilizados oito cães com pesos entre 15 e 30kg, distribuídos aleatoriamente em dois grupos denominados de I ou controle e II ou tratado. A articulação femorotibiopatelar esquerda foi imobilizada por 30 dias pelo método de transfixação percutânea tipo II, com retirada de aparelho de imobilização após esse período. Decorridas 48 horas da remoção, foi realizada a EENM nos cães do grupo II, cinco vezes por semana, com intervalo de 24 horas cada sessão, pelo período de 60 dias. Foram avaliadas a circunferência da coxa, a goniometria do joelho, a análise clínica da marcha, as enzimas creatina-quinase (CK e aspartato-amino-transferase (AST e a morfometria das fibras musculares em cortes transversais do músculo vasto lateral colhido mediante biópsia muscular. A EENM foi empregada no músculo quadríceps femoral na freqüência de 50Hz, duração de pulso de 300 milisegundos e relação de tempo on/off de 1:2. Quanto à morfometria das fibras do músculo vasto lateral, no grupo tratado houve aumento significativo (PLow frequency neuromuscular electrical stimulation (NMES was used on the femoral quadriceps of dogs with induced muscular atrophy and the occurrence of gain in mass in these muscles was evaluated. Eight dogs from 15 to 30kg were randomly distributed in two groups named I, or control; and II, or treated. For the induction of muscular atrophy, the left femoral-tibial-patellar joint was immobilized for 30 days by percutaneous transfixation type II. After 30 days, the immobilization device was removed. The NMES treatment began 48 hours after the removal of the immobilization device of the dogs of group II, and it was carried out five times per week with an interval of 24 hours between each session, for 60 days. The

  14. SoS Notebook: An Interactive Multi-Language Data Analysis Environment.

    Science.gov (United States)

    Peng, Bo; Wang, Gao; Ma, Jun; Leong, Man Chong; Wakefield, Chris; Melott, James; Chiu, Yulun; Du, Di; Weinstein, John N

    2018-05-22

    Complex bioinformatic data analysis workflows involving multiple scripts in different languages can be difficult to consolidate, share, and reproduce. An environment that streamlines the entire processes of data collection, analysis, visualization and reporting of such multi-language analyses is currently lacking. We developed Script of Scripts (SoS) Notebook, a web-based notebook environment that allows the use of multiple scripting language in a single notebook, with data flowing freely within and across languages. SoS Notebook enables researchers to perform sophisticated bioinformatic analysis using the most suitable tools for different parts of the workflow, without the limitations of a particular language or complications of cross-language communications. SoS Notebook is hosted at http://vatlab.github.io/SoS/ and is distributed under a BSD license. bpeng@mdanderson.org.

  15. Is the S.O.S. diagnostic algorithm applicable to creating highly safe protective systems?

    International Nuclear Information System (INIS)

    Drab, F.

    1994-01-01

    The S.O.S. diagnostic system is analyzed and compared with KOMPARACE and MIN-MAX type diagnostic systems. Designed for the identification of failed sensors, the S.O.S. dynamic algorithm is based on a digital monitoring of output signals from a pair of sensors measuring the same technological parameter. The last 3 output signal data from the two sensors are stored in the algorithm memory. The analysis indicates that S.O.S. is no major achievement in the field of diagnosis because its properties are nearly identical with those of the conventional MIN-MAX system. Some degradation failures of the sensor are incorrectly interpreted by the new algorithm, some failures are not detected at all. From this point of view the new algorithm is inferior to the KOMPARACE type algorithm. (J.B.). 2 figs., 5 refs

  16. A Small-Molecule Inducible Synthetic Circuit for Control of the SOS Gene Network without DNA Damage.

    Science.gov (United States)

    Kubiak, Jeffrey M; Culyba, Matthew J; Liu, Monica Yun; Mo, Charlie Y; Goulian, Mark; Kohli, Rahul M

    2017-11-17

    The bacterial SOS stress-response pathway is a pro-mutagenic DNA repair system that mediates bacterial survival and adaptation to genotoxic stressors, including antibiotics and UV light. The SOS pathway is composed of a network of genes under the control of the transcriptional repressor, LexA. Activation of the pathway involves linked but distinct events: an initial DNA damage event leads to activation of RecA, which promotes autoproteolysis of LexA, abrogating its repressor function and leading to induction of the SOS gene network. These linked events can each independently contribute to DNA repair and mutagenesis, making it difficult to separate the contributions of the different events to observed phenotypes. We therefore devised a novel synthetic circuit to unlink these events and permit induction of the SOS gene network in the absence of DNA damage or RecA activation via orthogonal cleavage of LexA. Strains engineered with the synthetic SOS circuit demonstrate small-molecule inducible expression of SOS genes as well as the associated resistance to UV light. Exploiting our ability to activate SOS genes independently of upstream events, we further demonstrate that the majority of SOS-mediated mutagenesis on the chromosome does not readily occur with orthogonal pathway induction alone, but instead requires DNA damage. More generally, our approach provides an exemplar for using synthetic circuit design to separate an environmental stressor from its associated stress-response pathway.

  17. Switching of the positive feedback for RAS activation by a concerted function of SOS membrane association domains.

    Science.gov (United States)

    Nakamura, Yuki; Hibino, Kayo; Yanagida, Toshio; Sako, Yasushi

    2016-01-01

    Son of sevenless (SOS) is a guanine nucleotide exchange factor that regulates cell behavior by activating the small GTPase RAS. Recent in vitro studies have suggested that an interaction between SOS and the GTP-bound active form of RAS generates a positive feedback loop that propagates RAS activation. However, it remains unclear how the multiple domains of SOS contribute to the regulation of the feedback loop in living cells. Here, we observed single molecules of SOS in living cells to analyze the kinetics and dynamics of SOS behavior. The results indicate that the histone fold and Grb2-binding domains of SOS concertedly produce an intermediate state of SOS on the cell surface. The fraction of the intermediated state was reduced in positive feedback mutants, suggesting that the feedback loop functions during the intermediate state. Translocation of RAF, recognizing the active form of RAS, to the cell surface was almost abolished in the positive feedback mutants. Thus, the concerted functions of multiple membrane-associating domains of SOS governed the positive feedback loop, which is crucial for cell fate decision regulated by RAS.

  18. SOS response activation and competence development are antagonistic mechanisms in Streptococcus thermophilus.

    Science.gov (United States)

    Boutry, Céline; Delplace, Brigitte; Clippe, André; Fontaine, Laetitia; Hols, Pascal

    2013-02-01

    Streptococcus includes species that either contain or lack the LexA-like repressor (HdiR) of the classical SOS response. In Streptococcus pneumoniae, a species which belongs to the latter group, SOS response inducers (e.g., mitomycin C [Mc] and fluoroquinolones) were shown to induce natural transformation, leading to the hypothesis that DNA damage-induced competence could contribute to genomic plasticity and stress resistance. Using reporter strains and microarray experiments, we investigated the impact of the SOS response inducers mitomycin C and norfloxacin and the role of HdiR on competence development in Streptococcus thermophilus. We show that both the addition of SOS response inducers and HdiR inactivation have a dual effect, i.e., induction of the expression of SOS genes and reduction of transformability. Reduction of transformability results from two different mechanisms, since HdiR inactivation has no major effect on the expression of competence (com) genes, while mitomycin C downregulates the expression of early and late com genes in a dose-dependent manner. The downregulation of com genes by mitomycin C was shown to take place at the level of the activation of the ComRS signaling system by an unknown mechanism. Conversely, we show that a ComX-deficient strain is more resistant to mitomycin C and norfloxacin in a viability plate assay, which indicates that competence development negatively affects the resistance of S. thermophilus to DNA-damaging agents. Altogether, our results strongly suggest that SOS response activation and competence development are antagonistic processes in S. thermophilus.

  19. A plasmid-encoded UmuD homologue regulates expression of Pseudomonas aeruginosa SOS genes.

    Science.gov (United States)

    Díaz-Magaña, Amada; Alva-Murillo, Nayeli; Chávez-Moctezuma, Martha P; López-Meza, Joel E; Ramírez-Díaz, Martha I; Cervantes, Carlos

    2015-07-01

    The Pseudomonas aeruginosa plasmid pUM505 contains the umuDC operon that encodes proteins similar to error-prone repair DNA polymerase V. The umuC gene appears to be truncated and its product is probably not functional. The umuD gene, renamed umuDpR, possesses an SOS box overlapped with a Sigma factor 70 type promoter; accordingly, transcriptional fusions revealed that the umuDpR gene promoter is activated by mitomycin C. The predicted sequence of the UmuDpR protein displays 23 % identity with the Ps. aeruginosa SOS-response LexA repressor. The umuDpR gene caused increased MMC sensitivity when transferred to the Ps. aeruginosa PAO1 strain. As expected, PAO1-derived knockout lexA-  mutant PW6037 showed resistance to MMC; however, when the umuDpR gene was transferred to PW6037, MMC resistance level was reduced. These data suggested that UmuDpR represses the expression of SOS genes, as LexA does. To test whether UmuDpR exerts regulatory functions, expression of PAO1 SOS genes was evaluated by reverse transcription quantitative PCR assays in the lexA-  mutant with or without the pUC_umuD recombinant plasmid. Expression of lexA, imuA and recA genes increased 3.4-5.3 times in the lexA-  mutant, relative to transcription of the corresponding genes in the lexA+ strain, but decreased significantly in the lexA- /umuDpR transformant. These results confirmed that the UmuDpR protein is a repressor of Ps. aeruginosa SOS genes controlled by LexA. Electrophoretic mobility shift assays, however, did not show binding of UmuDpR to 5' regions of SOS genes, suggesting an indirect mechanism of regulation.

  20. The SOS Suicide Prevention Program: Further Evidence of Efficacy and Effectiveness.

    Science.gov (United States)

    Schilling, Elizabeth A; Aseltine, Robert H; James, Amy

    2016-02-01

    This study replicated and extended previous evaluations of the Signs of Suicide (SOS) prevention program in a high school population using a more rigorous pre-test post-test randomized control design than used in previous SOS evaluations in high schools (Aseltine and DeMartino 2004; Aseltine et al. 2007). SOS was presented to an ethnically diverse group of ninth grade students in technical high schools in Connecticut. After controlling for the pre-test reports of suicide behaviors, exposure to the SOS program was associated with significantly fewer self-reported suicide attempts in the 3 months following the program. Ninth grade students in the intervention group were approximately 64% less likely to report a suicide attempt in the past 3 months compared with students in the control group. Similarly, exposure to the SOS program resulted in greater knowledge of depression and suicide and more favorable attitudes toward (1) intervening with friends who may be exhibiting signs of suicidal intent and (2) getting help for themselves if they were depressed or suicidal. In addition, high-risk SOS participants, defined as those with a lifetime history of suicide attempt, were significantly less likely to report planning a suicide in the 3 months following the program compared to lower-risk participants. Differential attrition is the most serious limitation of the study; participants in the intervention group who reported a suicide attempt in the previous 3 months at baseline were more likely to be missing at post-test than their counterparts in the control group.

  1. Mechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate.

    Science.gov (United States)

    Lee, Young Kwang; Low-Nam, Shalini T; Chung, Jean K; Hansen, Scott D; Lam, Hiu Yue Monatrice; Alvarez, Steven; Groves, Jay T

    2017-04-28

    The guanine nucleotide exchange factor (GEF) Son of Sevenless (SOS) plays a critical role in signal transduction by activating Ras. Here we introduce a single-molecule assay in which individual SOS molecules are captured from raw cell lysate using Ras-functionalized supported membrane microarrays. This enables characterization of the full-length SOS protein, which has not previously been studied in reconstitution due to difficulties in purification. Our measurements on the full-length protein reveal a distinct role of the C-terminal proline-rich (PR) domain to obstruct the engagement of allosteric Ras independently of the well-known N-terminal domain autoinhibition. This inhibitory role of the PR domain limits Grb2-independent recruitment of SOS to the membrane through binding of Ras·GTP in the SOS allosteric binding site. More generally, this assay strategy enables characterization of the functional behaviour of GEFs with single-molecule precision but without the need for purification.

  2. Chemical trapping and characterization of small oxoacids of sulfur (SOS) generated in aqueous oxidations of H2S.

    Science.gov (United States)

    Kumar, Murugaeson R; Farmer, Patrick J

    2018-04-01

    Small oxoacids of sulfur (SOS) are elusive molecules like sulfenic acid, HSOH, and sulfinic acid, HS(O)OH, generated during the oxidation of hydrogen sulfide, H 2 S, in aqueous solution. Unlike their alkyl homologs, there is a little data on their generation and speciation during H 2 S oxidation. These SOS may exhibit both nucleophilic and electrophilic reactivity, which we attribute to interconversion between S(II) and S(IV) tautomers. We find that SOS may be trapped in situ by derivatization with nucleophilic and electrophilic trapping agents and then characterized by high resolution LC MS. In this report, we compare SOS formation from H 2 S oxidation by a variety of biologically relevant oxidants. These SOS appear relatively long lived in aqueous solution, and thus may be involved in the observed physiological effects of H 2 S. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  3. Mechanism of SOS-induced targeted and untargeted mutagenesis in E. coli

    International Nuclear Information System (INIS)

    Maenhaut-Michel, G.

    1985-01-01

    This paper retraces the evolution of hypotheses concerning mechanisms of SOS induced mutagenesis. Moreover, it reports some recent data which support a new model for the mechanism of targeted and untargeted mutagenesis in E. coli. In summary, the SOS mutator effect, which is responsible for untargeted mutagenesis and perhaps for the misincorporation step in targeted mutagenesis, is believed to involve a fidelity function associated with DNA polymerase III and does not require the umuC gene product. umuC and umuD gene products are probably required specifically for elongation of DNA synthesis past blocking lesions, i.e. to allow mutagenic replication of damaged DNA

  4. Influence of the gene xthA in the activation of SOS response of Escherichia coli

    International Nuclear Information System (INIS)

    Dominguez M, V.

    2013-01-01

    The SOS response is one of the strategies that has Escherichia coli to counteract the lesions in the genetic material. The response is integrated for approximately 60 genes that when are activated they provide to the cell a bigger opportunity to survive. For the activation of this system is necessary that DNA regions of simple chain are generated, in such a way that most of the lesions should be processed, to be able to induce this answer. Some genes that intervene in this procedure, as recO, recB and recJ are recognized since when being exposed to the radiation, their activity SOS is smaller than in a wild strain. In previous works has been studied that to inactivate the genes that are involves in the lesions processing to generate DNA of simple chain, the SOS induction level diminishes with regard to a wild strain, but that when eliminating the genes that are involves directly in the repair, the SOS response increases. In this work a strain with defects in the gene xthA was built, which encodes for an endonuclease AP that participates in the repair mechanism by base excision and was evaluated their sensibility as the activity of the SOS response when exposing it to UV light and gamma radiation. The results showed that the lethality of the strain with the defect is very similar to the wild strain; while the activation level of the SOS response is bigger in comparison with the wild strain when being exposed to UV light; suggesting the existence of an enzyme that recognizes the lesions that produces this radiation, however, is not this the main repair channel, since the survival is similar to that of the wild strain. On the contrary, the results obtained with gamma radiation showed that the lethality diminishes in comparison to that of the wild strain, like the SOS activity; due surely to that the gene product intervenes in the repair for base excision, participating in the formation of the previous substrate to the activation of the SOS response. (Author)

  5. Compact pulse topology for adjustable high-voltage pulse generation using an SOS diode

    NARCIS (Netherlands)

    Driessen, A.B.J.M.; Heesch, van E.J.M.; Huiskamp, T.; Beckers, F.J.C.M.; Pemen, A.J.M.

    2014-01-01

    In this paper, a compact circuit topology is presented for pulsed power generation with a semiconductor opening switch (SOS). Such circuits require the generation of a fast forward current through the diode, followed by a reverse current that activates the recovery process. In general, magnetic

  6. Genotoxicity risk assessment of diversely substituted quinolines using the SOS chromotest.

    Science.gov (United States)

    Duran, Leidy Tatiana Díaz; Rincón, Nathalia Olivar; Galvis, Carlos Eduardo Puerto; Kouznetsov, Vladimir V; Lorenzo, Jorge Luis Fuentes

    2015-03-01

    Quinolines are aromatic nitrogen compounds with wide therapeutic potential to treat parasitic and microbial diseases. In this study, the genotoxicity of quinoline, 4-methylquinoline, 4-nitroquinoline-1-oxide (4-NQO), and diversely functionalized quinoline derivatives and the influence of the substituents (functional groups and/or atoms) on their genotoxicity were tested using the SOS chromotest. Quinoline derivatives that induce genotoxicity by the formation of an enamine epoxide structure did not induce the SOS response in Escherichia coli PQ37 cells, with the exception of 4-methylquinoline that was weakly genotoxic. The chemical nature of the substitution (C-5 to C-8: hydroxyl, nitro, methyl, isopropyl, chlorine, fluorine, and iodine atoms; C-2: phenyl and 3,4-methylenedioxyphenyl rings) of quinoline skeleton did not significantly modify compound genotoxicities; however, C-2 substitution with α-, β-, or γ-pyridinyl groups removed 4-methylquinoline genotoxicity. On the other hand, 4-NQO derivatives whose genotoxic mechanism involves reduction of the C-4 nitro group were strong inducers of the SOS response. Methyl and nitrophenyl substituents at C-2 of 4-NQO core affected the genotoxic potency of this molecule. The relevance of these results is discussed in relation to the potential use of the substituted quinolines. The work showed the sensitivity of SOS chromotest for studying structure-genotoxicity relationships and bioassay-guided quinoline synthesis. © 2013 Wiley Periodicals, Inc.

  7. Starvation, Together with the SOS Response, Mediates High Biofilm-Specific Tolerance to the Fluoroquinolone Ofloxacin

    Science.gov (United States)

    Bernier, Steve P.; Lebeaux, David; DeFrancesco, Alicia S.; Valomon, Amandine; Soubigou, Guillaume; Coppée, Jean-Yves; Ghigo, Jean-Marc; Beloin, Christophe

    2013-01-01

    High levels of antibiotic tolerance are a hallmark of bacterial biofilms. In contrast to well-characterized inherited antibiotic resistance, molecular mechanisms leading to reversible and transient antibiotic tolerance displayed by biofilm bacteria are still poorly understood. The physiological heterogeneity of biofilms influences the formation of transient specialized subpopulations that may be more tolerant to antibiotics. In this study, we used random transposon mutagenesis to identify biofilm-specific tolerant mutants normally exhibited by subpopulations located in specialized niches of heterogeneous biofilms. Using Escherichia coli as a model organism, we demonstrated, through identification of amino acid auxotroph mutants, that starved biofilms exhibited significantly greater tolerance towards fluoroquinolone ofloxacin than their planktonic counterparts. We demonstrated that the biofilm-associated tolerance to ofloxacin was fully dependent on a functional SOS response upon starvation to both amino acids and carbon source and partially dependent on the stringent response upon leucine starvation. However, the biofilm-specific ofloxacin increased tolerance did not involve any of the SOS-induced toxin–antitoxin systems previously associated with formation of highly tolerant persisters. We further demonstrated that ofloxacin tolerance was induced as a function of biofilm age, which was dependent on the SOS response. Our results therefore show that the SOS stress response induced in heterogeneous and nutrient-deprived biofilm microenvironments is a molecular mechanism leading to biofilm-specific high tolerance to the fluoroquinolone ofloxacin. PMID:23300476

  8. SOS System Induction Inhibits the Assembly of Chemoreceptor Signaling Clusters in Salmonella enterica.

    Science.gov (United States)

    Irazoki, Oihane; Mayola, Albert; Campoy, Susana; Barbé, Jordi

    2016-01-01

    Swarming, a flagellar-driven multicellular form of motility, is associated with bacterial virulence and increased antibiotic resistance. In this work we demonstrate that activation of the SOS response reversibly inhibits swarming motility by preventing the assembly of chemoreceptor-signaling polar arrays. We also show that an increase in the concentration of the RecA protein, generated by SOS system activation, rather than another function of this genetic network impairs chemoreceptor polar cluster formation. Our data provide evidence that the molecular balance between RecA and CheW proteins is crucial to allow polar cluster formation in Salmonella enterica cells. Thus, activation of the SOS response by the presence of a DNA-injuring compound increases the RecA concentration, thereby disturbing the equilibrium between RecA and CheW and resulting in the cessation of swarming. Nevertheless, when the DNA-damage decreases and the SOS response is no longer activated, basal RecA levels and thus polar cluster assembly are reestablished. These results clearly show that bacterial populations moving over surfaces make use of specific mechanisms to avoid contact with DNA-damaging compounds.

  9. Unexpected Cartilage Phenotype in CD4-Cre-Conditional SOS-Deficient Mice.

    Science.gov (United States)

    Guittard, Geoffrey; Gallardo, Devorah L; Li, Wenmei; Melis, Nicolas; Lui, Julian C; Kortum, Robert L; Shakarishvili, Nicholas G; Huh, Sunmee; Baron, Jeffrey; Weigert, Roberto; Kramer, Joshua A; Samelson, Lawrence E; Sommers, Connie L

    2017-01-01

    RAS signaling is central to many cellular processes and SOS proteins promote RAS activation. To investigate the role of SOS proteins in T cell biology, we crossed Sos1 f/f Sos2 -/- mice to CD4-Cre transgenic mice. We previously reported an effect of these mutations on T cell signaling and T cell migration. Unexpectedly, we observed nodules on the joints of greater than 90% of these mutant mice at 5 months of age, especially on the carpal joints. As the mice aged further, some also displayed joint stiffness, hind limb paralysis, and lameness. Histological analysis indicated that the abnormal growth in joints originated from dysplastic chondrocytes. Second harmonic generation imaging of the carpal nodules revealed that nodules were encased by rich collagen fibrous networks. Nodules formed in mice also deficient in RAG2, indicating that conventional T cells, which undergo rearrangement of the T cell antigen receptor, are not required for this phenotype. CD4-Cre expression in a subset of cells, either immune lineage cells (e.g., non-conventional T cells) or non-immune lineage cells (e.g., chondrocytes) likely mediates the dramatic phenotype observed in this study. Disruptions of genes in the RAS signaling pathway are especially likely to cause this phenotype. These results also serve as a cautionary tale to those intending to use CD4-Cre transgenic mice to specifically delete genes in conventional T cells.

  10. Tolerance of Escherichia coli to fluoroquinolone antibiotics depends on specific components of the SOS response pathway.

    Science.gov (United States)

    Theodore, Alyssa; Lewis, Kim; Vulic, Marin

    2013-12-01

    Bacteria exposed to bactericidal fluoroquinolone (FQ) antibiotics can survive without becoming genetically resistant. Survival of these phenotypically resistant cells, commonly called "persisters," depends on the SOS gene network. We have examined mutants in all known SOS-regulated genes to identify functions essential for tolerance in Escherichia coli. The absence of DinG and UvrD helicases and the Holliday junction processing enzymes RuvA and RuvB leads to a decrease in survival. Analysis of the respective mutants indicates that, in addition to repair of double-strand breaks, tolerance depends on the repair of collapsed replication forks and stalled transcription complexes. Mutation in recF results in increased survival, which identifies RecAF recombination as a poisoning mechanism not previously linked to FQ lethality. DinG acts upstream of SOS promoting its induction, whereas RuvAB participates in repair only. UvrD directly promotes all repair processes initiated by FQ-induced damage and prevents RecAF-dependent misrepair, making it one of the crucial SOS functions required for tolerance.

  11. CMOS/SOS RAM transient radiation upset and ''inversion'' effect investigation

    International Nuclear Information System (INIS)

    Nikiforov, A.Y.; Poljakov, I.V.

    1996-01-01

    The Complementary Metal-Oxide-Semiconductor/Silicon-on-Sapphire Random Access Memory (CMOS/SOS RAM) transient upset and inversion effect were investigated with pulsed laser, pulsed voltage generator and low-intensity light simulators. It was found that the inversion of information occurs due to memory cell photocurrents simultaneously with the power supply voltage drop transfer to memory cells outputs

  12. Induction of sos response in Escherichia Coli cells by gamma rays

    International Nuclear Information System (INIS)

    Fuentes Lorenzo, J.L.; Padron Soler, E.; Martin Hernandez, G.; Perez Tamayo, N.; del Sol Abascal, E.R.; Almeida Varela, E.

    1996-01-01

    The kinetics of sos response induction in Escherichia Coli cells was studied by means of the gene fusion SfiA:LacZ. In these cells, the specific beta galactosidase activity and the cellular growth rate showed an exponential behaviour. The sensitivity of the GC 2181 starin to gamma irradiation is equal to Do -1= 0.00088/Gy. The beta galactosidase activity

  13. Proceedings of the NKS/SOS-2 seminar on risk informed principles

    International Nuclear Information System (INIS)

    Pulkkinen, U.; Simola, K.

    1999-09-01

    The aim of this NKS/SOS-2 seminar was to present the status and plans of applications of Risk Informed Principles both by nuclear authorities and industry in Finland and Sweden. Furthermore, views from the off-shore industry were presented. (EHS)

  14. Paraísos fiscales en la globalización financiera

    Directory of Open Access Journals (Sweden)

    Alberto Garzón Espinosa

    2011-10-01

    Full Text Available Los paraísos fiscales son espacios financieros caracterizados ante todo por su baja o nula tributación. En este artículo examinaremos con detalle el uso de los mismos por parte de los agentes económicos, centrándonos especialmente en los bancos y los fondos de inversión colectiva. No obstante, como elementos clave de un nuevo contexto financiero los paraísos fiscales han tenido un papel fundamental en la gestación y expansión de todas las crisis financieras recientes, razón por la cual también estudiaremos las consecuencias que la existencia misma de los paraísos fiscales tiene sobre la economía y el sistema financiero.Palabras clave: Paraísos fiscales, globalización financiera, neoliberalismo_______________Abstract:The tax haven are financial spaces which it characterize for its shorts taxations. In this article we will analyze the use of that by the economic agent, specially the banks and the funds of collective investment. However, like key elements of the new financial context, the tax haven was been a leading role in gestation and expansion of all financial crisis of our days. For that, we will study the consequences of this fact in the economy and financial system.Keywords: tax haven, financial globalization, neoliberalism.

  15. Monitoring Ras Interactions with the Nucleotide Exchange Factor Son of Sevenless (Sos) Using Site-specific NMR Reporter Signals and Intrinsic Fluorescence*

    Science.gov (United States)

    Vo, Uybach; Vajpai, Navratna; Flavell, Liz; Bobby, Romel; Breeze, Alexander L.; Embrey, Kevin J.; Golovanov, Alexander P.

    2016-01-01

    The activity of Ras is controlled by the interconversion between GTP- and GDP-bound forms partly regulated by the binding of the guanine nucleotide exchange factor Son of Sevenless (Sos). The details of Sos binding, leading to nucleotide exchange and subsequent dissociation of the complex, are not completely understood. Here, we used uniformly 15N-labeled Ras as well as [13C]methyl-Met,Ile-labeled Sos for observing site-specific details of Ras-Sos interactions in solution. Binding of various forms of Ras (loaded with GDP and mimics of GTP or nucleotide-free) at the allosteric and catalytic sites of Sos was comprehensively characterized by monitoring signal perturbations in the NMR spectra. The overall affinity of binding between these protein variants as well as their selected functional mutants was also investigated using intrinsic fluorescence. The data support a positive feedback activation of Sos by Ras·GTP with Ras·GTP binding as a substrate for the catalytic site of activated Sos more weakly than Ras·GDP, suggesting that Sos should actively promote unidirectional GDP → GTP exchange on Ras in preference of passive homonucleotide exchange. Ras·GDP weakly binds to the catalytic but not to the allosteric site of Sos. This confirms that Ras·GDP cannot properly activate Sos at the allosteric site. The novel site-specific assay described may be useful for design of drugs aimed at perturbing Ras-Sos interactions. PMID:26565026

  16. High-throughput screening identifies small molecules that bind to the RAS:SOS:RAS complex and perturb RAS signaling.

    Science.gov (United States)

    Burns, Michael C; Howes, Jennifer E; Sun, Qi; Little, Andrew J; Camper, DeMarco V; Abbott, Jason R; Phan, Jason; Lee, Taekyu; Waterson, Alex G; Rossanese, Olivia W; Fesik, Stephen W

    2018-05-01

    K-RAS is mutated in approximately 30% of human cancers, resulting in increased RAS signaling and tumor growth. Thus, RAS is a highly validated therapeutic target, especially in tumors of the pancreas, lung and colon. Although directly targeting RAS has proven to be challenging, it may be possible to target other proteins involved in RAS signaling, such as the guanine nucleotide exchange factor Son of Sevenless (SOS). We have previously reported on the discovery of small molecules that bind to SOS1, activate SOS-mediated nucleotide exchange on RAS, and paradoxically inhibit ERK phosphorylation (Burns et al., PNAS, 2014). Here, we describe the discovery of additional, structurally diverse small molecules that also bind to SOS1 in the same pocket and elicit similar biological effects. We tested >160,000 compounds in a fluorescence-based assay to assess their effects on SOS-mediated nucleotide exchange. X-Ray structures revealed that these small molecules bind to the CDC25 domain of SOS1. Compounds that elicited high levels of nucleotide exchange activity in vitro increased RAS-GTP levels in cells, and inhibited phospho ERK levels at higher treatment concentrations. The identification of structurally diverse SOS1 binding ligands may assist in the discovery of new molecules designed to target RAS-driven tumors. Copyright © 2018 Elsevier Inc. All rights reserved.

  17. Characterization of the Burkholderia thailandensis SOS response by using whole-transcriptome shotgun sequencing.

    Science.gov (United States)

    Ulrich, Ricky L; Deshazer, David; Kenny, Tara A; Ulrich, Melanie P; Moravusova, Anna; Opperman, Timothy; Bavari, Sina; Bowlin, Terry L; Moir, Donald T; Panchal, Rekha G

    2013-10-01

    The bacterial SOS response is a well-characterized regulatory network encoded by most prokaryotic bacterial species and is involved in DNA repair. In addition to nucleic acid repair, the SOS response is involved in pathogenicity, stress-induced mutagenesis, and the emergence and dissemination of antibiotic resistance. Using high-throughput sequencing technology (SOLiD RNA-Seq), we analyzed the Burkholderia thailandensis global SOS response to the fluoroquinolone antibiotic, ciprofloxacin (CIP), and the DNA-damaging chemical, mitomycin C (MMC). We demonstrate that a B. thailandensis recA mutant (RU0643) is ∼4-fold more sensitive to CIP in contrast to the parental strain B. thailandensis DW503. Our RNA-Seq results show that CIP and MMC treatment (P SOS response were induced and include lexA, uvrA, dnaE, dinB, recX, and recA. At the genome-wide level, we found an overall decrease in gene expression, especially for genes involved in amino acid and carbohydrate transport and metabolism, following both CIP and MMC exposure. Interestingly, we observed the upregulation of several genes involved in bacterial motility and enhanced transcription of a B. thailandensis genomic island encoding a Siphoviridae bacteriophage designated E264. Using B. thailandensis plaque assays and PCR with B. mallei ATCC 23344 as the host, we demonstrate that CIP and MMC exposure in B. thailandensis DW503 induces the transcription and translation of viable bacteriophage in a RecA-dependent manner. This is the first report of the SOS response in Burkholderia spp. to DNA-damaging agents. We have identified both common and unique adaptive responses of B. thailandensis to chemical stress and DNA damage.

  18. EFEITO DO DISSELENETO DE DIFENILA SOBRE A TOXICIDADE INDUZIDA POR CLORETO DE MERCÚRIO EM CAMUNDONGOS

    OpenAIRE

    Ricardo Brandão

    2008-01-01

    O mercúrio (Hg) é um elemento ainda sem função fisiológica no organismo humano, sendo tóxico aos seres vivos. Este metal possui ampla aplicação na indústria sendo, portanto, bastante importante na exposição ocupacional e ambiental. A toxicidade do mercúrio depende da forma deste metal e pode afetar inúmeros órgãos, tais como o cérebro, os rins e o fígado e, ainda, causar alterações hematológicas e imunológicas. O estresse oxidativo parece estar envolvido na toxicidade induzida ...

  19. Efeitos de um minuto e dez minutos de deambulação em ratos com artrite induzida por adjuvante completo de Freund sobre os sintomas de dor e edema

    Directory of Open Access Journals (Sweden)

    Raquel Pinheiro Gomes

    2014-04-01

    Full Text Available Este estudo avaliou o efeito de dois protocolos de exercício na nocicepção, edema e migração celular em ratos com artrite induzida por CFA. Ratos Wistar fêmeas (200 - 250 g, n = 50 foram induzidos à monoartrite por adjuvante completo de Freund (CFA, Mycobacterium butyricum; 0,5 mg/mL; 50 μL na articulação do joelho direito (TF; n = 24 ou tornozelo direito (TT; n = 26. A incapacitação articular foi mensurada pelo tempo de elevação da pata (TEP; s em 1 minuto de avaliação. O edema do joelho ou tornozelo foi avaliado pela medida do diâmetro articular (AD, cm e pelo edema de pata (EP, mL, respectivamente. Ambos foram avaliados durante 10 dias consecutivos. Dois protocolos de exercício foram realizados: (a exercício constante (TF, n = 6; TT, n = 6, realizando 1 minuto diário de exercício no cilindro (3 r.p.m.; (b exercício variável (TF, n = 6; TT, n = 7, exercício com aumento de 1 minuto por dia, totalizando 10 minutos no último dia. Os grupos-controle (TF, n = 12; TT, n = 13 não realizaram exercício. Após 10 dias, os animais foram eutanasiados para contagem total (células/mm3 e diferencial (mononucleares e polimorfos nucleares; células/mm3 de leucócitos do tecido inflamado. O exercício variável inibiu a incapacitação e o edema em ambas as articulações. Entretanto, reduziu a migração total de leucócitos apenas na articulação TF. O exercício constante inibiu o edema nas duas articulações e reduziu a migração total de leucócitos da articulação TT. Porém, não reduziu a incapacitação. O exercício variável pareceu ser mais efetivo em reduzir os parâmetros inflamatórios em comparação com o exercício constante.

  20. Influence of very short patch mismatch repair on SOS inducing lesions after aminoglycoside treatment in Escherichia coli.

    Science.gov (United States)

    Baharoglu, Zeynep; Mazel, Didier

    2014-01-01

    Low concentrations of aminoglycosides induce the SOS response in Vibrio cholerae but not in Escherichia coli. In order to determine whether a specific factor present in E. coli prevents this induction, we developed a genetic screen where only SOS inducing mutants are viable. We identified the vsr gene coding for the Vsr protein of the very short patch mismatch repair (VSPR) pathway. The effect of mismatch repair (MMR) mutants was also studied. We propose that lesions formed upon aminoglycoside treatment are preferentially repaired by VSPR without SOS induction in E. coli and by MMR when VSPR is impaired. Copyright © 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

  1. Avaliação ecocardiográfica de gatos com tirotoxicose induzida Echocardiographic evaluation of cats with induced thyrotoxicosis

    Directory of Open Access Journals (Sweden)

    Daniel Capucho de Oliveira

    2010-04-01

    Full Text Available O hipertiroidismo é uma doença multissistêmica caracterizada pela elevação dos níveis séricos dos hormônios tireoidianos, sendo uma endocrinopatia comum em gatos. O objetivo deste trabalho foi avaliar possíveis alterações cardíacas pelo exame ecocardiográfico em gatos com tirotoxicose induzida. Foram utilizados nove gatos adultos, sem distinção de sexo ou raça e sem alterações que pudessem interferir nos resultados do estudo. Para indução à tirotoxicose, foram administrados, por via oral, comprimidos, na dose de 150µg kg-1 de levotiroxina sódica, a cada 24 horas, durante 70 dias. Os exames foram realizados em dois momentos: um momento antes da indução e outro ao término do protocolo experimental. Dos parâmetros avaliados, foi observada diminuição estatisticamente significativa nos valores de diâmetro interno do ventrículo esquerdo, tanto na sístole, quanto na diástole. O diâmetro diastólico (DDVE médio nos animais variou de 14,97mm, no momento inicial, para 13,27mm, no momento final. Os valores médios do diâmetro sistólico (DSVE variaram de 8,92mm para 7,92mm, nos momentos inicial e final, respectivamente. Os outros parâmetros avaliados não sofreram alterações. Dessa forma, já foi possível caracterizar alterações cardiovasculares secundárias ao excesso dos hormônios tiroidianos nesse curto prazo de indução, porém não havendo a instalação de um quadro de insuficiência cardíaca congestiva. Ressalta-se a necessidade de um diagnóstico precoce do hipertiroidismo felino para evitar a evolução dos efeitos cardiovasculares dessa enfermidade.Hyperthyroidism is a multisystemic disease arising from the excessive serum levels of the thyroid hormones, being a common endocrinopathy in cats. This study aimed at the evaluation of possible cardiac abnormalities by the echocardiographic exam in cats with induced thyrotoxicosis. Nine adult cats, regardless of sex or race and without alterations that could

  2. Anatomopathological study of cardiomyopathy induced by doxorubicin in rats Estudo anatomopatológico da miocardiopatia induzida pela doxorrubicina em ratos

    Directory of Open Access Journals (Sweden)

    José Carlos Dorsa Vieira Pontes

    2010-04-01

    Full Text Available PURPOSE: The development of an experimental model of myocardiopathy induced by Doxorubicin in rats. METHODS: 16 wistar male rats were randomized in two groups: Group I (placebo and Group II (Doxorubicin - 5mg/kg. After six months, the animals were subjected to cardiotomy and their hearts were weighted and submitted to transversal cuts, from which fragments for a macro and micro study were obtained. These fragments were studied considering their external and internal diameters and the thickness of the left ventricle (LV. The histological pieces were analyzed for the presence of fibrosis, cytoplasmic vacuolization, necrosis and size of nucleus variation. Data obtained was submitted to statistical analysis with Student's t test. RESULTS: The hearts of the animals in Group II increased 41% in relation to their weight; 33% in the internal diameter and 14% in the external diameter of the LV cavity; and 24% in the thickness of the wall. Fibrosis of the myocardial tissue was observed in 75% of the animals of Group II; all the animals presented miocyte cytoplasmatic vacuolization; myocardial necrosis was present in 75% of the animals; and 87/% presented variation in the size of myocite nuclei. The presence of polymorphonuclear cells was also observed. CONCLUSION: Doxorubicin was effective in the promotion of macro and microscopic alterations in the cardiac tissue of rats, possibly constituting a model for the experimental study of myocardiopathy.OBJETIVO: Desenvolver um modelo experimental de miocardiopatia induzida por doxorrubicina em ratos. MÉTODOS: 16 ratos Wistar machos foram randomizados em 2 grupos: Grupo I (placebo e Grupo II (doxorrubicina 5mg/kg. Após 6 meses, os animais foram submetidos a cardiotomia e seus corações foram pesados e submetidos a cortes transversais. Estes fragmentos foram estudados considerando seus diâmetros externos e internos e a espessura do ventrículo esquerdo. As peças histológicas foram analisadas quanto

  3. Genetic characterization of the inducible SOS-like system of Bacillus subtilis

    Energy Technology Data Exchange (ETDEWEB)

    Love, P.E.; Yasbin, R.E.

    1984-12-01

    The SOS-like system of Bacillus subtilis consists of several coordinately induced phenomena which are expressed after cellular insult such as DNA damage of inhibition of DNA replication. Mutagenesis of the bacterial chromosomes and the development of maintenance of competence also appear to be involved in the SOS-like response in this bacterium. The genetic characterization of the SOS-like system has involved an analysis of (i) the effects of various DNA repair mutations on the expression of inducible phenomena and (ii) the tsi-23 mutation, which renders host strains thermally inducible for each of the SOS-like functions. Bacterial filamentation was unaffected by any of the DNA repair mutations studied. In contrast, the induction of prophage after thermal or UV pretreatment was abolished in strains carrying the recE4, recA1, recB2, or recG13 mutation. The Weigle reactivation of UV-damaged bacteriophage was also inhibited by the recE4, recA1, recB2, or recG13 mutation, whereas levels of Weigle reactivation were lower in strains which carried the uvrA42, polA5, or rec-961 mutation than in the DNA repair-proficient strain. Strains which carried the recE4 mutation were incapable of chromosomal DNA-mediated transformation, and the frequency of this event was decreased in strains carrying recA1, recB2, or tsi-23 mutation. Plasmid DNA transformation efficiency was decreased only in strains carrying the tsi-23 mutation in addition to the recE4, recA1, or recB2 mutation. The results indicate that the SOS-like system of B. subtilis is regulated at different levels by two or more gene products. In this report, the current data regarding the genetic regulation of inducible phenomena are summarized, and a model is proposed to explain the mechanism of SOS-like induction in B. subtillis. 50 references, 3 figures, 6 tables.

  4. Atomic orbital-based SOS-MP2 with tensor hypercontraction. I. GPU-based tensor construction and exploiting sparsity.

    Science.gov (United States)

    Song, Chenchen; Martínez, Todd J

    2016-05-07

    We present a tensor hypercontracted (THC) scaled opposite spin second order Møller-Plesset perturbation theory (SOS-MP2) method. By using THC, we reduce the formal scaling of SOS-MP2 with respect to molecular size from quartic to cubic. We achieve further efficiency by exploiting sparsity in the atomic orbitals and using graphical processing units (GPUs) to accelerate integral construction and matrix multiplication. The practical scaling of GPU-accelerated atomic orbital-based THC-SOS-MP2 calculations is found to be N(2.6) for reference data sets of water clusters and alanine polypeptides containing up to 1600 basis functions. The errors in correlation energy with respect to density-fitting-SOS-MP2 are less than 0.5 kcal/mol for all systems tested (up to 162 atoms).

  5. Atomic orbital-based SOS-MP2 with tensor hypercontraction. I. GPU-based tensor construction and exploiting sparsity

    Energy Technology Data Exchange (ETDEWEB)

    Song, Chenchen; Martínez, Todd J. [Department of Chemistry and the PULSE Institute, Stanford University, Stanford, California 94305 (United States); SLAC National Accelerator Laboratory, Menlo Park, California 94025 (United States)

    2016-05-07

    We present a tensor hypercontracted (THC) scaled opposite spin second order Møller-Plesset perturbation theory (SOS-MP2) method. By using THC, we reduce the formal scaling of SOS-MP2 with respect to molecular size from quartic to cubic. We achieve further efficiency by exploiting sparsity in the atomic orbitals and using graphical processing units (GPUs) to accelerate integral construction and matrix multiplication. The practical scaling of GPU-accelerated atomic orbital-based THC-SOS-MP2 calculations is found to be N{sup 2.6} for reference data sets of water clusters and alanine polypeptides containing up to 1600 basis functions. The errors in correlation energy with respect to density-fitting-SOS-MP2 are less than 0.5 kcal/mol for all systems tested (up to 162 atoms).

  6. Dynamic studies of H-Ras•GTPγS interactions with nucleotide exchange factor Sos reveal a transient ternary complex formation in solution.

    Science.gov (United States)

    Vo, Uybach; Vajpai, Navratna; Embrey, Kevin J; Golovanov, Alexander P

    2016-07-14

    The cycling between GDP- and GTP- bound forms of the Ras protein is partly regulated by the binding of Sos. The structural/dynamic behavior of the complex formed between activated Sos and Ras at the point of the functional cycle where the nucleotide exchange is completed has not been described to date. Here we show that solution NMR spectra of H-Ras∙GTPγS mixed with a functional fragment of Sos (Sos(Cat)) at a 2:1 ratio are consistent with the formation of a rather dynamic assembly. H-Ras∙GTPγS binding was in fast exchange on the NMR timescale and retained a significant degree of molecular tumbling independent of Sos(Cat), while Sos(Cat) also tumbled largely independently of H-Ras. Estimates of apparent molecular weight from both NMR data and SEC-MALS revealed that, at most, only one H-Ras∙GTPγS molecule appears stably bound to Sos. The weak transient interaction between Sos and the second H-Ras∙GTPγS may provide a necessary mechanism for complex dissociation upon the completion of the native GDP → GTP exchange reaction, but also explains measurable GTP → GTP exchange activity of Sos routinely observed in in vitro assays that use fluorescently-labelled analogs of GTP. Overall, the data presents the first dynamic snapshot of Ras functional cycle as controlled by Sos.

  7. Tratamento da leishmaniose tegumentar americana pelo niridazol

    Directory of Open Access Journals (Sweden)

    Miroslau Constante Baranski

    1970-08-01

    Full Text Available Foi empregue o niridazol um derivado do nitrotiazol, em doze pacientes com leishmaniose tegumentar americana. Em todos êles, o diagnóstico clínico da doença foi confirmado pela biópsia das lesões e em oito dêles também pela positividade da intradetermorreação de Montenegro. Oito pacientes tinham lesões mucosas metastáticas em atividade e lesões cutâneas iniciais cicatrizadas há tempo mais ou menos longo. Dois apresentavam concomitância de lesões cutâneas e mucosas em atividade, e dois tinham lesões cutâneas exclusivas. A duração da doença variou de 2 meses a 32 anos. A posologia diária do niridazol foi uniformememente de 25 mg/kg de pêso corpóreo. O medicamento foi administrado por via oral, em duas tomadas diárias, sempre com o doente internado em hospital. Quando a tolerância o permitia, o paciente recebia cinco séries de tratamento de 10 dias de duração cada, intercaladas por períodos áe suspensão da droga de 10 dias entre uma série e outra. Isso foi possível em 10 dos 12 pacientes. O tratamento foi bem tolerado em 5 doentes e de tolerância regular em 4. Mal tolerado em um paciente pela ocorrência de alucinações e excitação mental e interrompido pela péssima tolerância em dois doentes devido ao aparecimento de convulsões generalizadas com perda da consciência. O tratamento acompanhou-se de grande incidência, de efeitos colaterais. A ocorrência de para-efeitos mais intensos não foi devida às más condições hepáticas. Provas de função hepática. hemogramas, estudos bioquímicos do sangue e exames de urina, realizados antes, durante e após o tratamento, não revelaram alterações significativas. Biópsias hepáticas por punção com agulha em dez doentes, prévias e posteriores ao tratamento, não detectaram lesões hepáticas que pudessem ser atribuídas à medicação. O seguimento dos doentes prolongou-se pelo prazo de dois a 36 meses apos o tratamento. Alterações eletrocardiogr

  8. Inhibitors of LexA Autoproteolysis and the Bacterial SOS Response Discovered by an Academic-Industry Partnership.

    Science.gov (United States)

    Mo, Charlie Y; Culyba, Matthew J; Selwood, Trevor; Kubiak, Jeffrey M; Hostetler, Zachary M; Jurewicz, Anthony J; Keller, Paul M; Pope, Andrew J; Quinn, Amy; Schneck, Jessica; Widdowson, Katherine L; Kohli, Rahul M

    2018-03-09

    The RecA/LexA axis of the bacterial DNA damage (SOS) response is a promising, yet nontraditional, drug target. The SOS response is initiated upon genotoxic stress, when RecA, a DNA damage sensor, induces LexA, the SOS repressor, to undergo autoproteolysis, thereby derepressing downstream genes that can mediate DNA repair and accelerate mutagenesis. As genetic inhibition of the SOS response sensitizes bacteria to DNA damaging antibiotics and decreases acquired resistance, inhibitors of the RecA/LexA axis could potentiate our current antibiotic arsenal. Compounds targeting RecA, which has many mammalian homologues, have been reported; however, small-molecules targeting LexA autoproteolysis, a reaction unique to the prokaryotic SOS response, have remained elusive. Here, we describe the logistics and accomplishments of an academic-industry partnership formed to pursue inhibitors against the RecA/LexA axis. A novel fluorescence polarization assay reporting on RecA-induced self-cleavage of LexA enabled the screening of 1.8 million compounds. Follow-up studies on select leads show distinct activity patterns in orthogonal assays, including several with activity in cell-based assays reporting on SOS activation. Mechanistic assays demonstrate that we have identified first-in-class small molecules that specifically target the LexA autoproteolysis step in SOS activation. Our efforts establish a realistic example for navigating academic-industry partnerships in pursuit of anti-infective drugs and offer starting points for dedicated lead optimization of SOS inhibitors that could act as adjuvants for current antibiotics.

  9. RpoS plays a central role in the SOS induction by sub-lethal aminoglycoside concentrations in Vibrio cholerae.

    Science.gov (United States)

    Baharoglu, Zeynep; Krin, Evelyne; Mazel, Didier

    2013-01-01

    Bacteria encounter sub-inhibitory concentrations of antibiotics in various niches, where these low doses play a key role for antibiotic resistance selection. However, the physiological effects of these sub-lethal concentrations and their observed connection to the cellular mechanisms generating genetic diversification are still poorly understood. It is known that, unlike for the model bacterium Escherichia coli, sub-minimal inhibitory concentrations (sub-MIC) of aminoglycosides (AGs) induce the SOS response in Vibrio cholerae. SOS is induced upon DNA damage, and since AGs do not directly target DNA, we addressed two issues in this study: how sub-MIC AGs induce SOS in V. cholerae and why they do not do so in E. coli. We found that when bacteria are grown with tobramycin at a concentration 100-fold below the MIC, intracellular reactive oxygen species strongly increase in V. cholerae but not in E. coli. Using flow cytometry and gfp fusions with the SOS regulated promoter of intIA, we followed AG-dependent SOS induction. Testing the different mutation repair pathways, we found that over-expression of the base excision repair (BER) pathway protein MutY relieved this SOS induction in V. cholerae, suggesting a role for oxidized guanine in AG-mediated indirect DNA damage. As a corollary, we established that a BER pathway deficient E. coli strain induces SOS in response to sub-MIC AGs. We finally demonstrate that the RpoS general stress regulator prevents oxidative stress-mediated DNA damage formation in E. coli. We further show that AG-mediated SOS induction is conserved among the distantly related Gram negative pathogens Klebsiella pneumoniae and Photorhabdus luminescens, suggesting that E. coli is more of an exception than a paradigm for the physiological response to antibiotics sub-MIC.

  10. Modeling of kinetics of the inducible protein complexes of the SOS system in bacteria E. coli which realize TLS process

    International Nuclear Information System (INIS)

    Belov, O.V.

    2008-01-01

    The mathematical model describing kinetics of the inducible genes of the protein complexes, formed during SOS response in bacteria Escherichia coli is developed. Within the bounds of developed approaches the auxiliary mathematical model describing changes in concentrations of the dimers, which are the components of final protein complexes, is developed. The solutions of both models are based on the experimental data concerning expression of the basic genes of the SOS system in bacteria Escherichia coli

  11. Mutation-Specific Mechanisms of Hyperactivation of Noonan Syndrome SOS Molecules Detected with Single-molecule Imaging in Living Cells.

    Science.gov (United States)

    Nakamura, Yuki; Umeki, Nobuhisa; Abe, Mitsuhiro; Sako, Yasushi

    2017-10-26

    Noonan syndrome (NS) is a congenital hereditary disorder associated with developmental and cardiac defects. Some patients with NS carry mutations in SOS, a guanine nucleotide exchange factor (GEF) for the small GTPase RAS. NS mutations have been identified not only in the GEF domain, but also in various domains of SOS, suggesting that multiple mechanisms disrupt SOS function. In this study, we examined three NS mutations in different domains of SOS to clarify the abnormality in its translocation to the plasma membrane, where SOS activates RAS. The association and dissociation kinetics between SOS tagged with a fluorescent protein and the living cell surface were observed in single molecules. All three mutants showed increased affinity for the plasma membrane, inducing excessive RAS signalling. However, the mechanisms by which their affinity was increased were specific to each mutant. Conformational disorder in the resting state, increased probability of a conformational change on the plasma membrane, and an increased association rate constant with the membrane receptor are the suggested mechanisms. These different properties cause the specific phenotypes of the mutants, which should be rescuable with different therapeutic strategies. Therefore, single-molecule kinetic analyses of living cells are useful for the pathological analysis of genetic diseases.

  12. Functional consequences of inducible genetic elements from the p53 SOS response in a mammalian organ system.

    Science.gov (United States)

    Guthrie, O'neil W

    2017-10-01

    In response to DNA damage from ultraviolet (UV) radiation, bacteria deploy the SOS response in order to limit cell death. This bacterial SOS response is characterized by an increase in the recA gene that transactivates expression of multiple DNA repair genes. The current series of experiments demonstrate that a mammalian organ system (the cochlea) that is not evolutionarily conditioned to UV radiation can elicit SOS responses that are reminiscent of that of bacteria. This mammalian SOS response is characterized by an increase in the p53 gene with activation of multiple DNA repair genes that harbor p53 response elements in their promoters. Furthermore, the experimental results provide support for the notion of a convergent trigger paradox, where independent SOS triggers facilitate disparate physiologic sequelae (loss vs. recovery of function). Therefore, it is proposed that the mammalian SOS response is multifunctional and manipulation of this endogenous response could be exploited in future biomedical interventions. Copyright © 2017 Elsevier Inc. All rights reserved.

  13. istSOS, a new sensor observation management system: software architecture and a real-case application for flood protection

    Directory of Open Access Journals (Sweden)

    M. Cannata

    2015-11-01

    Full Text Available istSOS (Istituto scienze della Terra Sensor Observation Service is an implementation of the Sensor Observation Service (SOS standard from the Open Geospatial Consortium. The development of istSOS started in 2009 in order to provide a simple implementation of the SOS for the management, provision and integration of hydro-meteorological data collected in Canton Ticino (Southern Switzerland. istSOS is an Open Source, entirely written in Python and based on reliable software like PostgreSQL/PostGIS and Apache/mod_wsgi. This paper illustrates the latest software enhancements, including a RESTful Web service and a Web-based graphical user interface, which enable a better and simplified interaction with measurements and SOS service settings. The robustness of the implemented solution has been validated in a real-case application: the Verbano Lake Early Warning System. In this application, near real-time data have to be exchanged by inter-regional partners and used in a hydrological model for lake level forecasting and flooding hazard assessment. This system is linked with a dedicated geoportal used by the civil protection for the management, alert and protection of the population and the assets of the Locarno area. Practical considerations, technical issues and foreseen improvements are presented and discussed.

  14. Overexpression of the PtSOS2 gene improves tolerance to salt stress in transgenic poplar plants.

    Science.gov (United States)

    Yang, Yang; Tang, Ren-Jie; Jiang, Chun-Mei; Li, Bei; Kang, Tao; Liu, Hua; Zhao, Nan; Ma, Xu-Jun; Yang, Lei; Chen, Shao-Liang; Zhang, Hong-Xia

    2015-09-01

    In higher plants, the salt overly sensitive (SOS) signalling pathway plays a crucial role in maintaining ion homoeostasis and conferring salt tolerance under salinity condition. Previously, we functionally characterized the conserved SOS pathway in the woody plant Populus trichocarpa. In this study, we demonstrate that overexpression of the constitutively active form of PtSOS2 (PtSOS2TD), one of the key components of this pathway, significantly increased salt tolerance in aspen hybrid clone Shanxin Yang (Populus davidiana × Populus bolleana). Compared to the wild-type control, transgenic plants constitutively expressing PtSOS2TD exhibited more vigorous growth and produced greater biomass in the presence of high concentrations of NaCl. The improved salt tolerance was associated with a decreased Na(+) accumulation in the leaves of transgenic plants. Further analyses revealed that plasma membrane Na(+) /H(+) exchange activity and Na(+) efflux in transgenic plants were significantly higher than those in the wild-type plants. Moreover, transgenic plants showed improved capacity in scavenging reactive oxygen species (ROS) generated by salt stress. Taken together, our results suggest that PtSOS2 could serve as an ideal target gene to genetically engineer salt-tolerant trees. © 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

  15. Effects of lidocaine on lipopolysaccharide-induced synovitis in horses Efeitos da lidocaína em sinovite induzida por lipopolissacarídeo, em eqüinos

    Directory of Open Access Journals (Sweden)

    R.C. Campebell

    2004-06-01

    ísticas bioquímicas e celulares do líquido sinovial foram avaliadas nos mesmos tempos. A resposta inflamatória local e sistêmica foi mensurada pela concentração do TNF-alfa no soro e líquido sinovial. Observou-se aumento da concentração do TNF-alfa nas articulações injetadas com LPS às 3h no grupo A e de 1,30 às 3h no grupo B. Concluiu-se que o LPS induziu o processo inflamatório e que a lidocaína não inibiu nem atenuou a sinovite induzida pelo LPS, nem a síntese e liberação de TNF-alfa .

  16. Treinamento em natação com baixa intensidade não protege músculo esquelético contra lesões induzidas por exercício exaustivo em ratos

    Directory of Open Access Journals (Sweden)

    Edson da Silva

    2011-06-01

    Full Text Available Enquanto o exercício aeróbico regular promove adaptações benéficas ao músculo esquelético, o exercício físico exaustivo induz lesões musculares. O objetivo deste estudo foi verificar se um programa de natação com baixa intensidade é capaz de proteger músculos esqueléticos contra lesões induzidas por exercício exaustivo. Ratos Wistar (peso: 376,50 ± 4,36g; idade: 90 dias foram divididos aleatoriamente em quatro grupos: controle sedentário (CS; sedentário submetido a teste de exaustão (SE; treinado em natação (TN; treinado em natação submetido a teste de exaustão (TNE. Animais dos grupos TN e TNE foram submetidos a um programa de natação sem sobrecarga por 90 minutos/dia, cinco dias/semana, durante 17 semanas. Após este período, os grupos SE e TNE foram submetidos a um teste de exaustão em natação. Após eutanásia, fragmentos dos músculos sóleo e reto femoral foram coletados e submetidos à análise histológica e de proteínas de choque térmico (HSP70. Os resultados mostraram que o tempo até a exaustão foi maior no grupo TNE que no SE (125,0 ± 6,0 vs. 90,0 ± 8,5min, respectivamente, P < 0,05. Os níveis de lactato sanguíneo durante o teste e exaustão foram menores no grupo TNE que no SE (5,31 ± 0,22 vs. 8,76 ± 0,59mmol/L, respectivamente, P < 0,05. A frequência de fibras lesadas nos músculos foi maior nos grupos SE (sóleo: 34,86 ± 0,04; reto femoral: 37,57 ± 0,07 e TNE (sóleo: 41,57 ± 0,08; reto femoral: 39,57 ± 0,05, comparada aos grupos CS (sóleo: 13,88 ± 0,81; reto femoral: 16,75 ± 0,79 e TN (sóleo: 24,14 ± 0,06; reto femoral: 24,0 ± 0,05, respectivamente (P < 0,05. Não houve diferença significativa nos níveis de HSP70 dos músculos analisados entre os quatro grupos. Concluimos que apesar do treinamento em natação melhorar o desempenho dos animais no teste de exaustão, não promoveu proteção aos seus músculos esqueléticos contra as lesões induzidas pelo exercício exaustivo

  17. Regulation of the E. coli SOS response by the lexA gene product

    International Nuclear Information System (INIS)

    Brent, R.

    1983-01-01

    In an Escherichia coli that is growing normally, transcription of many genes is repressed by the product of the lexA gene. If cellular DNA is damaged, proteolytically competent recA protein (recA protease) inactivates lexA protein and these genes are induced. Many of the cellular phenomena observed during the cellular response to DNA damage (the SOS response) are the consequence of the expression of these lexA-prepressed genes. Since the SOS response of E. coli has recently been the subject of a comprehensive review, in this paper I would like to concentrate on some modifications to the picture based on new data. 12 references, 2 figures

  18. Suppression of SOS-inducing activity of chemical mutagens by metabolites from microbial transformation of (-)-isolongifolene.

    Science.gov (United States)

    Sakata, Kazuki; Oda, Yoshimitsu; Miyazawa, Mitsuo

    2010-02-24

    In this study, biotransformation of (-)-isolongifolene (1) by Glomerella cingulata and suppressive effect on umuC gene expression by chemical mutagens 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide) and aflatoxin B(1) (AFB(1)) of the SOS response in Salmonella typhimurium TA1535/pSK1002 were investigated. Initially, 1 was carried out the microbial transformation by G. cingulata. The result found that 1 was converted into (-)-isolongifolen-9-one (2), (-)-(2S)-13-hydroxy-isolongifolen-9-one (3), and (-)-(4R)-4-hydroxy-isolongifolen-9-one (4) by G. cingulata, and their conversion rates were 60, 25, and 15%, respectively. The metabolites suppressed the SOS-inducing activity of furylfuramid and AFB(1) in the umu test. Comound 2 showed gene expression by chemical mutagens furylfuramide and AFB(1) was suppressed 54 and 50% at <0.5 mM, respectively. Compound 2 is the most effective compound in this experiment.

  19. Architecture and performance of radiation-hardened 64-bit SOS/MNOS memory

    International Nuclear Information System (INIS)

    Kliment, D.C.; Ronen, R.S.; Nielsen, R.L.; Seymour, R.N.; Splinter, M.R.

    1976-01-01

    This paper discusses the circuit architecture and performance of a nonvolatile 64-bit MNOS memory fabricated on silicon on sapphire (SOS). The circuit is a test vehicle designed to demonstrate the feasibility of a high-performance, high-density, radiation-hardened MNOS/SOS memory. The array is organized as 16 words by 4 bits and is fully decoded. It utilizes a two-(MNOS) transistor-per-bit cell and differential sensing scheme and is realized in PMOS static resistor load logic. The circuit was fabricated and tested as both a fast write random access memory (RAM) and an electrically alterable read only memory (EAROM) to demonstrate design and process flexibility. Discrete device parameters such as retention, circuit electrical characteristics, and tolerance to total dose and transient radiation are presented

  20. The Verrucomicrobia LexA-binding Motif: Insights into the Evolutionary Dynamics of the SOS Response

    Directory of Open Access Journals (Sweden)

    Ivan Erill

    2016-07-01

    Full Text Available The SOS response is the primary bacterial mechanism to address DNA damage, coordinating multiple cellular processes that include DNA repair, cell division and translesion synthesis. In contrast to other regulatory systems, the composition of the SOS genetic network and the binding motif of its transcriptional repressor, LexA, have been shown to vary greatly across bacterial clades, making it an ideal system to study the co-evolution of transcription factors and their regulons. Leveraging comparative genomics approaches and prior knowledge on the core SOS regulon, here we define the binding motif of the Verrucomicrobia, a recently described phylum of emerging interest due to its association with eukaryotic hosts. Site directed mutagenesis of the Verrucomicrobium spinosum recA promoter confirms that LexA binds a 14 bp palindromic motif with consensus sequence TGTTC-N4-GAACA. Computational analyses suggest that recognition of this novel motif is determined primarily by changes in base-contacting residues of the third alpha helix of the LexA helix-turn-helix DNA binding motif. In conjunction with comparative genomics analysis of the LexA regulon in the Verrucomicrobia phylum, electrophoretic shift assays reveal that LexA binds to operators in the promoter region of DNA repair genes and a mutagenesis cassette in this organism, and identify previously unreported components of the SOS response. The identification of tandem LexA-binding sites generating instances of other LexA-binding motifs in the lexA gene promoter of Verrucomicrobia species leads us to postulate a novel mechanism for LexA-binding motif evolution. This model, based on gene duplication, successfully addresses outstanding questions in the intricate co-evolution of the LexA protein, its binding motif and the regulatory network it controls.

  1. The SOS Chromotest applied for screening plant antigenotoxic agents against ultraviolet radiation.

    Science.gov (United States)

    Fuentes, J L; García Forero, A; Quintero Ruiz, N; Prada Medina, C A; Rey Castellanos, N; Franco Niño, D A; Contreras García, D A; Córdoba Campo, Y; Stashenko, E E

    2017-09-13

    In this work, we investigated the usefulness of the SOS Chromotest for screening plant antigenotoxic agents against ultraviolet radiation (UV). Fifty Colombian plant extracts obtained by supercritical fluid (CO 2 ) extraction, twelve plant extract constituents (apigenin, carvacrol, β-caryophyllene, 1,8-cineole, citral, p-cymene, geraniol, naringenin, pinocembrin, quercetin, squalene, and thymol) and five standard antioxidant and/or photoprotective agents (curcumin, epigallocatechin gallate, resveratrol, α-tocopherol, and Trolox®) were evaluated for their genotoxicity and antigenotoxicity against UV using the SOS Chromotest. None of the plant extracts, constituents or agents were genotoxic in the SOS Chromotest at tested concentrations. Based on the minimal extract concentration that significantly inhibited UV-genotoxicity (CIG), five plant extracts were antigenotoxic against UV as follows: Baccharis nítida (16 μg mL -1 ) = Solanum crotonifolium (16 μg mL -1 ) > Hyptis suaveolens (31 μg mL -1 ) = Persea caerulea (31 μg mL -1 ) > Lippia origanoides (62 μg mL -1 ). Based on CIG values, the flavonoid compounds showed the highest antigenotoxic potential as follows: apigenin (7 μM) > pinocembrin (15 μM) > quercetin (26 μM) > naringenin (38 μM) > epigallocatechin gallate (108 μM) > resveratrol (642 μM). UV-genotoxicity inhibition with epigallocatechin gallate, naringenin and resveratrol was related to its capability for inhibiting protein synthesis. A correlation analysis between compound antigenotoxicity estimates and antioxidant activity evaluated by the oxygen radical absorbance capacity (ORAC) assay showed that these activities were not related. The usefulness of the SOS Chromotest for bioprospecting of plant antigenotoxic agents against UV was discussed.

  2. The Verrucomicrobia LexA-Binding Motif: Insights into the Evolutionary Dynamics of the SOS Response.

    Science.gov (United States)

    Erill, Ivan; Campoy, Susana; Kılıç, Sefa; Barbé, Jordi

    2016-01-01

    The SOS response is the primary bacterial mechanism to address DNA damage, coordinating multiple cellular processes that include DNA repair, cell division, and translesion synthesis. In contrast to other regulatory systems, the composition of the SOS genetic network and the binding motif of its transcriptional repressor, LexA, have been shown to vary greatly across bacterial clades, making it an ideal system to study the co-evolution of transcription factors and their regulons. Leveraging comparative genomics approaches and prior knowledge on the core SOS regulon, here we define the binding motif of the Verrucomicrobia, a recently described phylum of emerging interest due to its association with eukaryotic hosts. Site directed mutagenesis of the Verrucomicrobium spinosum recA promoter confirms that LexA binds a 14 bp palindromic motif with consensus sequence TGTTC-N4-GAACA. Computational analyses suggest that recognition of this novel motif is determined primarily by changes in base-contacting residues of the third alpha helix of the LexA helix-turn-helix DNA binding motif. In conjunction with comparative genomics analysis of the LexA regulon in the Verrucomicrobia phylum, electrophoretic shift assays reveal that LexA binds to operators in the promoter region of DNA repair genes and a mutagenesis cassette in this organism, and identify previously unreported components of the SOS response. The identification of tandem LexA-binding sites generating instances of other LexA-binding motifs in the lexA gene promoter of Verrucomicrobia species leads us to postulate a novel mechanism for LexA-binding motif evolution. This model, based on gene duplication, successfully addresses outstanding questions in the intricate co-evolution of the LexA protein, its binding motif and the regulatory network it controls.

  3. Specificity determinants for autoproteolysis of LexA, a key regulator of bacterial SOS mutagenesis.

    Science.gov (United States)

    Mo, Charlie Y; Birdwell, L Dillon; Kohli, Rahul M

    2014-05-20

    Bacteria utilize the tightly regulated stress response (SOS) pathway to respond to a variety of genotoxic agents, including antimicrobials. Activation of the SOS response is regulated by a key repressor-protease, LexA, which undergoes autoproteolysis in the setting of stress, resulting in derepression of SOS genes. Remarkably, genetic inactivation of LexA's self-cleavage activity significantly decreases acquired antibiotic resistance in infection models and renders bacteria hypersensitive to traditional antibiotics, suggesting that a mechanistic study of LexA could help inform its viability as a novel target for combating acquired drug resistance. Despite structural insights into LexA, a detailed knowledge of the enzyme's protease specificity is lacking. Here, we employ saturation and positional scanning mutagenesis on LexA's internal cleavage region to analyze >140 mutants and generate a comprehensive specificity profile of LexA from the human pathogen Pseudomonas aeruginosa (LexAPa). We find that the LexAPa active site possesses a unique mode of substrate recognition. Positions P1-P3 prefer small hydrophobic residues that suggest specific contacts with the active site, while positions P5 and P1' show a preference for flexible glycine residues that may facilitate the conformational change that permits autoproteolysis. We further show that stabilizing the β-turn within the cleavage region enhances LexA autoproteolytic activity. Finally, we identify permissive positions flanking the scissile bond (P4 and P2') that are tolerant to extensive mutagenesis. Our studies shed light on the active site architecture of the LexA autoprotease and provide insights that may inform the design of probes of the SOS pathway.

  4. Novel Escherichia coli umuD′ Mutants: Structure-Function Insights into SOS Mutagenesis

    Science.gov (United States)

    McLenigan, Mary; Peat, Thomas S.; Frank, Ekaterina G.; McDonald, John P.; Gonzalez, Martín; Levine, Arthur S.; Hendrickson, Wayne A.; Woodgate, Roger

    1998-01-01

    Although it has been 10 years since the discovery that the Escherichia coli UmuD protein undergoes a RecA-mediated cleavage reaction to generate mutagenically active UmuD′, the function of UmuD′ has yet to be determined. In an attempt to elucidate the role of UmuD′ in SOS mutagenesis, we have utilized a colorimetric papillation assay to screen for mutants of a hydroxylamine-treated, low-copy-number umuD′ plasmid that are unable to promote SOS-dependent spontaneous mutagenesis. Using such an approach, we have identified 14 independent umuD′ mutants. Analysis of these mutants revealed that two resulted from promoter changes which reduced the expression of wild-type UmuD′, three were nonsense mutations that resulted in a truncated UmuD′ protein, and the remaining nine were missense alterations. In addition to the hydroxylamine-generated mutants, we have subcloned the mutations found in three chromosomal umuD1, umuD44, and umuD77 alleles into umuD′. All 17 umuD′ mutants resulted in lower levels of SOS-dependent spontaneous mutagenesis but varied in the extent to which they promoted methyl methanesulfonate-induced mutagenesis. We have attempted to correlate these phenotypes with the potential effect of each mutation on the recently described structure of UmuD′. PMID:9721309

  5. Structural inhibition and reactivation of Escherichia coli septation by elements of the SOS and TER pathways

    International Nuclear Information System (INIS)

    Dopazo, A.; Tormo, A.; Aldea, M.; Vicente, M.

    1987-01-01

    The inhibition of cell division caused by induction of the SOS pathway in Escherichia coli structurally blocks septation, as deduced from two sets of results. Potential septation sites active at the time of SOS induction became inactivated, while those initiated during the following doubling time were active. Penicillin resistance increased in wild-type UV light-irradiated cells, a behavior similar to that observed in mutants in which structural blocks were introduced by inactivation of FtsA. Potential septation sites that have been structurally blocked by either the SOS division inhibitor, furazlocillin inhibition of PBP3, or inactivation of a TER pathway component, FtsA3, could be reactivated one doubling time after removal of the inhibitory agent in the presence of an active lon gene product. Reactivation of potential septation sites blocked by the presence of an inactivated FtsA3 was significantly lower when the lon protease was not active, suggesting that Lon plays a role in the removal of inactivated TER pathway products from the blocked potential septation sites

  6. Induction of the SOS response in ultraviolet-irradiated Escherichia coli analyzed by dynamics of LexA, RecA and SulA proteins

    International Nuclear Information System (INIS)

    Aksenov, S.V.

    1999-01-01

    The SOS response in Escherichia coli is induced after DNA-damaging treatments including ultraviolet light. Regulation of the SOS response is accomplished through specific interaction of the two SOS regulator proteins, LexA and RecA. In ultraviolet light treated cells nucleotide excision repair is the major system that removes the induced lesions from the DNA. Here, induction of the SOS response in Escherichia coli with normal and impaired excision repair function is studied by simulation of intracellular levels of regulatory LexA and RecA proteins, and SulA protein. SulA protein is responsible for SOS-inducible cell division inhibition. Results of the simulations show that nucleotide excision repair influences time-courses of LexA , RecA and SulA induction by modulating the dynamics of RecA protein distribution between its normal and SOS-activated forms

  7. The SOS-LUX-LAC-FLUORO-Toxicity-test on the International Space Station (ISS).

    Science.gov (United States)

    Rabbow, E; Rettberg, P; Baumstark-Khan, C; Horneck, G

    2003-01-01

    In the 21st century, an increasing number of astronauts will visit the International Space Station (ISS) for prolonged times. Therefore it is of utmost importance to provide necessary basic knowledge concerning risks to their health and their ability to work on the station and during extravehicular activities (EVA) in free space. It is the aim of one experiment of the German project TRIPLE-LUX (to be flown on the ISS) to provide an estimation of health risk resulting from exposure of the astronauts to the radiation in space inside the station as well as during extravehicular activities on one hand, and of exposure of astronauts to unavoidable or as yet unknown ISS-environmental genotoxic substances on the other. The project will (i) provide increased knowledge of the biological action of space radiation and enzymatic repair of DNA damage, (ii) uncover cellular mechanisms of synergistic interaction of microgravity and space radiation and (iii) examine the space craft milieu with highly specific biosensors. For these investigations, the bacterial biosensor SOS-LUX-LAC-FLUORO-Toxicity-test will be used, combining the SOS-LUX-Test invented at DLR Germany (Patent) with the commercially available LAC-FLUORO-Test. The SOS-LUX-Test comprises genetically modified bacteria transformed with the pBR322-derived plasmid pPLS-1. This plasmid carries the promoterless lux operon of Photobacterium leiognathi as a reporter element under control of the DNA-damage dependent SOS promoter of ColD as sensor element. This system reacts to radiation and other agents that induce DNA damages with a dose dependent measurable emission of bioluminescence of the transformed bacteria. The analogous LAC-FLUORO-Test has been developed for the detection of cellular responses to cytotoxins. It is based on the constitutive expression of green fluorescent protein (GFP) mediated by the bacterial protein expression vector pGFPuv (Clontech, Palo Alto, USA). In response to cytotoxic agents, this system

  8. First evidence on the validity and reliability of the Safety Organizing Scale-Nursing Home version (SOS-NH).

    Science.gov (United States)

    Ausserhofer, Dietmar; Anderson, Ruth A; Colón-Emeric, Cathleen; Schwendimann, René

    2013-08-01

    The Safety Organizing Scale is a valid and reliable measure on safety behaviors and practices in hospitals. This study aimed to explore the psychometric properties of the Safety Organizing Scale-Nursing Home version (SOS-NH). In a cross-sectional analysis of staff survey data, we examined validity and reliability of the 9-item Safety SOS-NH using American Educational Research Association guidelines. This substudy of a larger trial used baseline survey data collected from staff members (n = 627) in a variety of work roles in 13 nursing homes (NHs) in North Carolina and Virginia. Psychometric evaluation of the SOS-NH revealed good response patterns with low average of missing values across all items (3.05%). Analyses of the SOS-NH's internal structure (eg, comparative fit indices = 0.929, standardized root mean square error of approximation = 0.045) and consistency (composite reliability = 0.94) suggested its 1-dimensionality. Significant between-facility variability, intraclass correlations, within-group agreement, and design effect confirmed appropriateness of the SOS-NH for measurement at the NH level, justifying data aggregation. The SOS-NH showed discriminate validity from one related concept: communication openness. Initial evidence regarding validity and reliability of the SOS-NH supports its utility in measuring safety behaviors and practices among a wide range of NH staff members, including those with low literacy. Further psychometric evaluation should focus on testing concurrent and criterion validity, using resident outcome measures (eg, patient fall rates). Copyright © 2013 American Medical Directors Association, Inc. All rights reserved.

  9. DinB Upregulation Is the Sole Role of the SOS Response in Stress-Induced Mutagenesis in Escherichia coli

    Science.gov (United States)

    Galhardo, Rodrigo S.; Do, Robert; Yamada, Masami; Friedberg, Errol C.; Hastings, P. J.; Nohmi, Takehiko; Rosenberg, Susan M.

    2009-01-01

    Stress-induced mutagenesis is a collection of mechanisms observed in bacterial, yeast, and human cells in which adverse conditions provoke mutagenesis, often under the control of stress responses. Control of mutagenesis by stress responses may accelerate evolution specifically when cells are maladapted to their environments, i.e., are stressed. It is therefore important to understand how stress responses increase mutagenesis. In the Escherichia coli Lac assay, stress-induced point mutagenesis requires induction of at least two stress responses: the RpoS-controlled general/starvation stress response and the SOS DNA-damage response, both of which upregulate DinB error-prone DNA polymerase, among other genes required for Lac mutagenesis. We show that upregulation of DinB is the only aspect of the SOS response needed for stress-induced mutagenesis. We constructed two dinB(oc) (operator-constitutive) mutants. Both produce SOS-induced levels of DinB constitutively. We find that both dinB(oc) alleles fully suppress the phenotype of constitutively SOS-“off” lexA(Ind−) mutant cells, restoring normal levels of stress-induced mutagenesis. Thus, dinB is the only SOS gene required at induced levels for stress-induced point mutagenesis. Furthermore, although spontaneous SOS induction has been observed to occur in only a small fraction of cells, upregulation of dinB by the dinB(oc) alleles in all cells does not promote a further increase in mutagenesis, implying that SOS induction of DinB, although necessary, is insufficient to differentiate cells into a hypermutable condition. PMID:19270270

  10. Clinical and biochemical evaluation of the saliva of patients with xerostomia induced by radiotherapy Avaliação clínica e bioquímica da saliva de pacientes com xerostomia induzida por radioterapia

    Directory of Open Access Journals (Sweden)

    Cássio de Barros Pontes

    2004-03-01

    Full Text Available Clinical aspects and biochemical properties in the saliva of 21 patients prior to and following radiotherapy for head and neck cancer were evaluated (experimental group and compared with the same properties in a control group of 21 subjects free of cancer. Salivary flow was evaluated by measuring the time necessary, in seconds, for the output of 2 ml of stimulated saliva; and the buffering capacity changes were determined using a simple colorimetric method. Total salivary protein concentration was determined by the Bradford4 method. Amylase activity was measured by reducing sugars released from a soluble starch substrate, quantified by the dinitrosalicylic method. The electrophoretic profile was evaluated in polyacrylamid gel (12% SDS-PAGE using samples of 5 mg of salivary protein. A statistically significant reduction (p Foram avaliados alguns aspectos clínicos e algumas propriedades bioquímicas salivares de 21 pacientes, antes e após o tratamento radioterápico para câncer de cabeça e pescoço (grupo experimental e de 21 pacientes sem câncer (grupo controle. O fluxo salivar foi avaliado pelo tempo necessário (segundos para produção estimulada de 2 ml de saliva e a capacidade tamponante determinada frente à utilização de um método colorimétrico simples. A concentração de proteína total salivar foi determinada pelo método de Bradford4. A atividade da amilase foi mensurada através dos açúcares redutores liberados e quantificados pelo método do ácido dinitrossalicílico utilizando a glicose como substrato. O perfil eletroforético foi avaliado em gel de poliacrilamida (SDS-PAGE 12% para amostras salivares contendo 5 mg de proteína. Foi observada, no grupo experimental, redução estatisticamente significativa (p < 0,01 para o fluxo salivar (162,47 s ± 28,30 antes e 568,71 s ± 79,75 após e para a capacidade tamponante (pH 5,45 ± 0,14 antes e 4,40 ± 0,15 após. Não foi observada alteração estatisticamente

  11. Entendendo os mecanismos determinantes da lesão pulmonar induzida pela ventilação mecânica Understanding the mechanisms of ventilator-induced lung injury

    Directory of Open Access Journals (Sweden)

    Liliane M. Nardelli

    2007-12-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: A ventilação mecânica é considerada elemento básico de suporte de vida nas unidades de terapia intensiva e, indubitavelmente, essencial para os pacientes com lesão pulmonar aguda (LPA e síndrome do desconforto respiratório agudo (SDRA. Estudos experimentais demonstraram que a ventilação mecânica (VM com altos volumes e/ou altas pressões pode exacerbar ou iniciar uma lesão pulmonar, denominada lesão pulmonar associada à VM (LPAV ou lesão pulmonar induzida pelo ventilador (LPIV, respectivamente, com aspecto histológico similar ao da LPA/SDRA. CONTEÚDO: Realizou-se uma pesquisa sistemática dos artigos incluídos na MedLine e SciElo dos últimos 20 anos, que abordavam uma visão crítica dos principais mecanismos determinantes da LPIV. Dentre os principais mecanismos da LPAV/LPIV pode-se citar: volutrauma causado por hiperdistensão e expansão desigual das unidades alveolares em função de altas pressões transpulmonares ou volumes; aletectrauma resultante da abertura e fechamento cíclicos das vias aéreas distais e o biotrauma determinado pelo processo inflamatório conseqüente às estratégias ventilatórias lesivas adotadas. CONCLUSÕES: Os mecanismos responsáveis pelo volutrauma, atelectrauma e biotrauma devem ser bem entendidos para que se possa evitar a lesão associada à ventilação mecânica.BACKGROUND AND OBJECTIVES: Mechanical ventilation is considered a basic element of life support in the intensive care unit and is essential for patients with acute lung injury (ALI and acute respiratory distress syndrome (ARDS. Experimental studies have demonstrated that mechanical ventilation with high volumes and/or high pressures can exacerbate (VALI or induce lung injury (VILI with histological aspect similar to ALI/ARDS. CONTENTS: This systematic review included the literature on MedLine and SciElo database published in the last 20 years. In this review, we will highlight the most recent data on

  12. Terapia por Contensão Induzida (TCI em adolescentes com hemiparesia espástica: relato de caso

    Directory of Open Access Journals (Sweden)

    Julia Macruz Garcia

    Full Text Available INTRODUÇÃO: A Terapia por Contensão Induzida (TCI é um protocolo terapêutico que visa diminuir a assimetria de uso dos membros superiores. OBJETIVO: Verificar o efeito da TCI em dois adolescentes, com paralisia cerebral hemiparética (PCH. MATERIAIS E MÉTODOS: Dois adolescentes com PCH, de 12 e 14 anos de idade, receberam aplicação de TCI por três horas diárias durante três semanas. Este estudo apresentou desenho ABA experimental, com um mês de seguimento. Os adolescentes foram avaliados com a Teenager Motor Activity Log (TMAL e o Wolf Motor Function Test (WMFT; o avaliador esteve independente da intervenção e cegado quanto à ordem das avaliações. RESULTADOS: Houve melhora na quantidade, qualidade e espontaneidade de uso após a aplicação da TCI segundo a escala da TMAL. Os dois pacientes apresentaram diminuição no tempo de execução das tarefas do WMFT e foi observada também melhora na habilidade funcional. CONCLUSÃO: Este estudo mostra efeitos positivos da TCI em adolescentes com PCH. Ressalta-se, no entanto, que se fazem necessários ensaios clínicos para confirmar a eficácia da intervenção nessa população.

  13. Detecção de lesões neoplásicas induzidas em mucosa oral de hamster utilizando espectroscopia de fluorescência Detection of induced neoplastic lesions in the oral mucosa of hamsters using fluorescence spectroscopy

    Directory of Open Access Journals (Sweden)

    Landulfo Silveira Junior

    2004-09-01

    Full Text Available OBJETIVO: Este trabalho teve por objetivo a utilização da técnica de espectroscopia de fluorescência induzida por laser para a caracterização de tecido normal e neoplásico em mucosa jugal de hamster, visando diagnosticar tecidos neoplásicos in vivo. MÉTODOS: O carcinógeno DMBA foi aplicado na bochecha direita de 31 hamsters com 150 ± 10g, três vezes por semana durante 12 semanas. Um animal foi mantido como controle (sem aplicação da droga. Após este período, os animais foram submetidos à espectroscopia de fluorescência induzida por laser de argônio (488nm, acoplado a um cabo de fibras ópticas. A autofluorescência do tecido foi guiada pelo cabo de fibras e analisada por um espectrógrafo e uma câmera CCD 1024X256 pixels, cobrindo a faixa espectral de 550nm a 700nm. Os espectros foram coletados na área da lesão induzida e na bochecha contralateral (normal de todos os animais, além do animal de controle. Subseqüente à espectroscopia, foi realizada a biópsia da lesão para a análise histopatológica. Dois algoritmos de diagnóstico dos espectros de tecido neoplásico, baseados na razão entre regiões espectrais e na técnica de análise das componentes principais (PCA foram implementados. RESULTADOS: Foi demonstrada a existência de um pico intenso na região de 630nm nos tecidos neoplásicos (atribuído à protoporfirina IX, quando comparados com o tecido normal. O algoritmo baseado na razão entre regiões espectrais obteve 100% de sensibilidade e especificidade. O algoritmo baseado na PCA obteve 94% e 100% de sensibilidade e especificidade, respectivamente. CONCLUSÕES: Este trabalho indica que a autofluorescência de tecidos da mucosa oral poderá ser utilizada como uma técnica não-invasiva de diagnóstico, com alta sensibilidade e especificidade.OBJECTIVE: This work analyzes use of the Laser Induced Fluorescence Spectroscopy technique for characterization of normal and neoplastic tissue in the oral mucosa of

  14. Development of the software of the data taking system SOS for the SAPHIR experiment. Entwicklung der Software des Datennahmesystems SOS fuer das SAPHIR-Experiment

    Energy Technology Data Exchange (ETDEWEB)

    Manns, J.

    1989-02-01

    The data acquistion system SOS has been developed for the SAPHIR experiment at the Bonn stretcher ring ELSA. It can handle up to 280 kilobytes of data per second or a maximum triggerrate of 200 Hz. The multiprocessor based online system consists of twenty VIP-microprocessors and two VAX-computers. Each component of the SAPHIR experiment has at least one program in the online system to maintain special functions for this specific component. All of these programs can receive event data without interfering with the transfer of events to a mass storage for offline analysis. A special program SOL has been developed to serve as a user interface to the data acquisition system and as a status display for most of the programs of the online system. Using modern features like windowing and mouse control on a VAX-station the SAPHIR online SOL establishes an easy way of controlling the data acquisition system. (orig.).

  15. The SOS system like model to study the response to the ionizing radiation; El sistema SOS como modelo para estudiar la respuesta a la radiacion ionizante

    Energy Technology Data Exchange (ETDEWEB)

    Serment G, J.; Brena V, M., E-mail: jorge.serment@inin.gob.m [ININ, Departamento de Radiobiologia, Laboratorio de Genetica Microbiana, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico)

    2010-07-01

    One of the important aspects in the radiobiology is the study of the strategies that the alive beings follow to counteract the genetic lesions generated by ionizing radiation. Among these is the SOS response of the bacteria, a group of 60 genes approximately that is activated when they happen lesions in the DNA and whose causes and structural diversity are multiple. So, an interest point in this response is to elucidate as all this lesions diversity activates this response in turn since, the protein that begins the process only recognizes simple ruptures of the double helix. For this is comes off that in the great majority of the cases is required of a previous prosecution of the damages so that the structure is generated that shoots this system. In this work a brief summary of the contributions in this field is made by part of the Microbiology Laboratory of the Instituto Nacional de Investigaciones Nucleares (ININ). (Author)

  16. The SOS response is permitted in Escherichia coli strains deficient in the expression of the mazEF pathway.

    Science.gov (United States)

    Kalderon, Ziva; Kumar, Sathish; Engelberg-Kulka, Hanna

    2014-01-01

    The Escherichia coli (E. coli) SOS response is the largest, most complex, and best characterized bacterial network induced by DNA damage. It is controlled by a complex network involving the RecA and LexA proteins. We have previously shown that the SOS response to DNA damage is inhibited by various elements involved in the expression of the E. coli toxin-antitoxin mazEF pathway. Since the mazEF module is present on the chromosomes of most E. coli strains, here we asked: Why is the SOS response found in so many E. coli strains? Is the mazEF module present but inactive in those strains? We examined three E. coli strains used for studies of the SOS response, strains AB1932, BW25113, and MG1655. We found that each of these strains is either missing or inhibiting one of several elements involved in the expression of the mazEF-mediated death pathway. Thus, the SOS response only takes place in E. coli cells in which one or more elements of the E. coli toxin-antitoxin module mazEF or its downstream pathway is not functioning.

  17. Phosphotyrosine-mediated LAT assembly on membranes drives kinetic bifurcation in recruitment dynamics of the Ras activator SOS.

    Science.gov (United States)

    Huang, William Y C; Yan, Qingrong; Lin, Wan-Chen; Chung, Jean K; Hansen, Scott D; Christensen, Sune M; Tu, Hsiung-Lin; Kuriyan, John; Groves, Jay T

    2016-07-19

    The assembly of cell surface receptors with downstream signaling molecules is a commonly occurring theme in multiple signaling systems. However, little is known about how these assemblies modulate reaction kinetics and the ultimate propagation of signals. Here, we reconstitute phosphotyrosine-mediated assembly of extended linker for the activation of T cells (LAT):growth factor receptor-bound protein 2 (Grb2):Son of Sevenless (SOS) networks, derived from the T-cell receptor signaling system, on supported membranes. Single-molecule dwell time distributions reveal two, well-differentiated kinetic species for both Grb2 and SOS on the LAT assemblies. The majority fraction of membrane-recruited Grb2 and SOS both exhibit fast kinetics and single exponential dwell time distributions, with average dwell times of hundreds of milliseconds. The minor fraction exhibits much slower kinetics, extending the dwell times to tens of seconds. Considering this result in the context of the multistep process by which the Ras GEF (guanine nucleotide exchange factor) activity of SOS is activated indicates that kinetic stabilization from the LAT assembly may be important. This kinetic proofreading effect would additionally serve as a stochastic noise filter by reducing the relative probability of spontaneous SOS activation in the absence of receptor triggering. The generality of receptor-mediated assembly suggests that such effects may play a role in multiple receptor proximal signaling processes.

  18. Monitoring Ras Interactions with the Nucleotide Exchange Factor Son of Sevenless (Sos) Using Site-specific NMR Reporter Signals and Intrinsic Fluorescence.

    Science.gov (United States)

    Vo, Uybach; Vajpai, Navratna; Flavell, Liz; Bobby, Romel; Breeze, Alexander L; Embrey, Kevin J; Golovanov, Alexander P

    2016-01-22

    The activity of Ras is controlled by the interconversion between GTP- and GDP-bound forms partly regulated by the binding of the guanine nucleotide exchange factor Son of Sevenless (Sos). The details of Sos binding, leading to nucleotide exchange and subsequent dissociation of the complex, are not completely understood. Here, we used uniformly (15)N-labeled Ras as well as [(13)C]methyl-Met,Ile-labeled Sos for observing site-specific details of Ras-Sos interactions in solution. Binding of various forms of Ras (loaded with GDP and mimics of GTP or nucleotide-free) at the allosteric and catalytic sites of Sos was comprehensively characterized by monitoring signal perturbations in the NMR spectra. The overall affinity of binding between these protein variants as well as their selected functional mutants was also investigated using intrinsic fluorescence. The data support a positive feedback activation of Sos by Ras·GTP with Ras·GTP binding as a substrate for the catalytic site of activated Sos more weakly than Ras·GDP, suggesting that Sos should actively promote unidirectional GDP → GTP exchange on Ras in preference of passive homonucleotide exchange. Ras·GDP weakly binds to the catalytic but not to the allosteric site of Sos. This confirms that Ras·GDP cannot properly activate Sos at the allosteric site. The novel site-specific assay described may be useful for design of drugs aimed at perturbing Ras-Sos interactions. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  19. Cirrose experimental induzida pela inalação de tetracloreto de carbono: adaptação da técnica e avaliação da peroxidação lipídica

    Directory of Open Access Journals (Sweden)

    CREMONESE Ricardo Viégas

    2001-01-01

    Full Text Available Racional - Administração a longo prazo de tetracloreto de carbono é modelo experimental para produzir fibrose hepática. O estresse oxidativo parece ser o mecanismo envolvido na hepatoxicidade por tetracloreto de carbono, onde as espécies ativas de oxigênio têm importante papel na patogênese da fibrose hepática. Objetivos - Avaliar a eficácia de um modelo experimental de cirrose hepática induzida pela inalação de tetracloreto de carbono em ratos, bem como avaliar a peroxidação lipídica e as características do líquido de ascite neste modelo. Material e Métodos - Inicialmente, acompanhou-se a evolução dos achados histológicos, através da técnica de hematoxilina e eosina, mediante o uso de tetracloreto de carbono inalatório ao longo das diferentes semanas (5ª, 7ª, 9ª, 12ª. Posteriormente, ao final da 15ª semana de estudo, os ratos, então divididos em três grupos (controle; controle + fenobarbital; e tetracloreto de carbono + fenobarbital, foram avaliados em sua histologia hepática, peroxidação lipídica e as características do líquido de ascite. Para as análises de peroxidação lipídica utilizaram-se as técnicas de substâncias reativas ao ácido tiobarbitúrico e de quimiluminescência. No líquido de ascite avaliaram-se a citologia e a bacteriologia. Resultados - Observou-se entre a 12ª e 15ª semanas de inalação o estabelecimento de cirrose em 100% dos animais submetidos a inalação com tetracloreto de carbono, acompanhada de um aumento significante na peroxidação lipídica no fígado dos ratos inalados com tetracloreto de carbono. Evidenciou-se a presença de infecção do líquido de ascite em um dos sete casos nos quais esta estava presente. Conclusão - O método inalatório desenvolvido é eficaz na indução de cirrose hepática e formação de ascite, sendo o estresse oxidativo um dos principais mecanismos da indução de cirrose pelo tetracloreto de carbono.

  20. Apoptotic effects of inositol hexaphosphate on biomarker Itpr3 in induced colon rat carcinogenesis Efeito de apoptose do inositol hexafosfato no marcador biológico Itpr3 em carcinogênese induzida de colo em ratos

    Directory of Open Access Journals (Sweden)

    Marks Guido

    2008-04-01

    Full Text Available PURPOSE: To study the effect of the modulation of inositol hexaphosphate (IP6 in the biological immunohistochemistry expression of cellular signaling marker apoptosis, in model of carcinogenesis of colon induced by azoxymethane (AOM. METHODS: Wistar rats (N=112 distributed in 4 groups (n=28: Control; B, AOM (5 mg kg-1, 2x, to break week 3; C, IP6 (in water 1%, six weeks; D, IP6+AOM. Weekly euthanasia (n=7, from week three. Immunohistochemistry of ascendant colon with biological marker inositol 1,4,5 triphosphate receptor type III (Itpr3. Quantification of the immune-expression with use of computer-assisted image processing. Analysis statistics of the means between groups, weeks in groups, groups in weeks, and established significance when pOBJETIVO: Estudar os efeitos da modulação do inositol hexafosfato (IP6 na expressão imunoistoquímica de marcador biológico de sinalização celular de apoptose, em modelo de carcinogênese induzida pelo azoximetano (AOM. MÉTODOS: Ratos Wistar (N=112 distribuídos em 4 grupos (n=28: A, controle; B, AOM (5 mg Kg-1, 2x, a partir semana 3; C, IP6 (em água a 1%, seis semanas; D, IP6+AOM. Eutanásia semanal (n=7, a partir de semana três. Imunoistoquímica de colo ascendente com marcador biológico inositol 1,4,5 trisphosphate receptor type III (Itpr3. Quantificação da imunoexpressão com uso de processamento imagem assistida computador. Análise estatística da expressão média entre grupos, semanas em grupos e grupos em semanas, e estabelecido significância quando p<0.05. RESULTADOS: Evidenciou-se diferença significante entre grupos na expressão de Itpr3, p<0.0001; com diminuição Itpr3 de grupo BxD, p<0.001. CONCLUSÃO: O inositol hexafostato promove a modulação de marcador biológico com diminuição Itpr3 em carcinogênese de colo.

  1. Estudo das modificações oculares induzidas pelo implante estromal do anel de Ferrara em portadores de ceratocone Study of the ocular changes after stromal Ferrara ring implantation in patients with keratoconus

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    Paulo Ferrara de Almeida Cunha

    2003-08-01

    Full Text Available OBJETIVO: Comparar a acuidade visual com e sem correção, ceratometria média e equivalente esférico antes e após o implante do anel intraestromal de Ferrara em pacientes portadores de ceratocone intolerantes a lentes de contato. MÉTODOS: Foram analisados os primeiros 400 olhos com ceratocone tratados com o implante do anel de Ferrara, levando-se em consideração as alterações obtidas na curvatura corneana, acuidade visual e refração, bem como a estabilidade dos resultados no primeiro ano. RESULTADOS: Nos 400 olhos estudados, observamos redução média na curvatura corneana de 4,18 D, o equivalente esférico diminuiu 4,65 D quando comparamos os valores obtidos no pré-operatório e os do último exame realizado. A AV média não corrigida foi a que sofreu maior alteração, passando de PURPOSE: To compare the changes in corneal curvature, visual acuity and spherical equivalent before and after the implantation of the intrastromal corneal Ferrara ring in contact lens intolerant keratoconus patients. METHODS: The first 400 eyes treated with the Ferrara ring were evaluated according to the alterations obtained in the corneal curvature, visual acuity and refraction after surgery, as well as the stability of the results in the first year. RESULTS: In the 400-studied eyes, there was a mean reduction of 4.18 D in keratometry and 4.65 D in spherical equivalent. The mean uncorrected visual acuity showed the greatest improvement varying from less than 20/400 before to 20/100 after surgery. The mean BSCVA was 20/50 after the surgery. The more advanced keratoconus (III/IV groups showed the greatest reduction in keratometry. The correction remained stable along the period of the study in all groups. CONCLUSION: The Ferrara ring showed to produce reduction of myopia, regularization of the cornea and consequent corrections of the irregular astigmatism, disappearance of the keratoconic pattern and a significant improvement of the corrected and uncorrected visual acuity.

  2. Alterações no líquido sinovial do joelho de cães com osteoartrite induzida pelo modelo Pond e Nuki Synovial fluid changes in the dog knee with osteoarthritis induced by Pond and Nuki model

    Directory of Open Access Journals (Sweden)

    F. De Biasi

    2001-10-01

    Full Text Available Avaliaram-se as alterações do líquido sinovial do joelho de 19 cães submetidos à desmotomia unilateral do cruzado cranial, baseado no modelo experimental de Pond & Nuki, colhido e analisado aos 30 dias de pós-operatório. Cinco animais foram analisados novamente aos 90 dias de pós-operatório. As características analisadas foram volume, cor, aspecto, densidade, pH, proteínas, fibrinogênio, coágulo de mucina, contagem de hemácias e contagem total e diferencial de células nucleadas. O líquido sinovial apresentou padrão inflamatório que persistiu durante todo o período de observação, com efusão articular. Houve aumento na contagem de células nucleadas e na concentração de fibrinogênio aos 90 dias. A qualidade da precipitação da mucina apresentou melhora aos 90 dias quando comparada à de 30 dias, porém ainda marcadamente anormal. Foi possível concluir que até 90 dias de pós-operatório no modelo utilizado o fluído sinovial apresenta características inflamatórias e não degenerativas.The purpose of this study was to evaluate the changes in knee synovial fluid of 19 dogs with experimentally-induced cranial cruciate ligament rupture, based on Pond & Nuki model. Synovial fluid was achieved and analyzed at 30 days postoperative time. Five animals had further synovial fluid evaluation at 90 days postoperatively. The characteristics observed were color, turbidity, density, pH, protein, fibrinogen, mucin clot, cell count and citology. The synovial fluid exhibited an inflammatory pattern during the postoperative observation time and joint effusion was observed. An increased number of nucleated cells and increased fibrinogen concentration were observed at 90 days when compared to 30 days after desmotomy. Although severely abnormal, an improvement of the mucin clot was observed at 90 days. It can be concluded that synovial fluid in this experimental model, showed inflammatory and no degenerative changes until 90 days postoperative.

  3. Caracterização da AMPK/mTOR hipotalâmica na anorexia induzida pelo câncer = : Characterization of hypothalamic AMPK/mTOR in cancer-induced anorexia

    OpenAIRE

    Gustavo Duarte Pimentel

    2015-01-01

    Resumo: A teoria das doenças geradas por citocinas inflamatórias trouxe ao longo dos anos indícios que o organismo pode produzir citocinas que desempenham respostas biológicas benéficas ou prejudiciais. Com o passar dos anos ficou claro que a inflamação é um mecanismo chave na fisiopatologia do câncer. Interessantemente, diversos estudos sugerem que a AMPK e mTOR hipotalâmica, importantes moléculas no controle do balanço energética também seja responsável por modular a inflamação e anorexia. ...

  4. Biotransformación del pelo residual de curtiembres

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    Ruth Viviana Numpaque

    2016-12-01

    Full Text Available La industria de curtiembres genera una alta carga contaminante representada por residuos líquidos, sólidos y gaseosos. Entre los residuos sólidos, el pelo residual es vertido directamente al río Bogotá y/o en rellenos sanitarios, desencadenando serios conflictos de orden ambiental, social y económico. El objetivo de esta investigación fue evaluar la capacidad de los microorganismos del EM y Agroplux en la transformación del pelo residual en compost. Se evaluaron tres tratamientos con pelo residual y materiales orgánicos de plaza en diferentes porcentajes y dos controles, utilizando el diseño completamente al azar, con tres repeticiones. Durante el compostaje se determinaron las variables temperatura, formación de agregados, pH y conductividad eléctrica. El desarrollo de la temperatura ocurrió en ciclos que concordaron con los volteos y re-inoculaciones. En el tercer ciclo el mayor incremento de temperatura fue obtenido en los tres tratamientos, cuando ya se había agotado la mayor parte de los residuos orgánicos. La formación de agregados indicó que el proceso se llevó a efecto principalmente en el tratamiento con 20% de pelo residual más 80% de residuos orgánicos. Además de su color oscuro y la ausencia de olor y fragmentos, el compost producido mediante dicho tratamiento mostró la conductividad eléctrica más baja y un pH casi neutro. Se concluye que los microorganismos del EM y Agroplux tienen capacidad para transformar el pelo residual en compost y por lo tanto representan una alternativa viable para la continuación del funcionamiento de la industria de curtiembres en Villapinzón (Cundinamarca y en otras regiones de Colombia.

  5. Interaction of caffeine with the SOS response pathway in Escherichia coli.

    Science.gov (United States)

    Whitney, Alyssa K; Weir, Tiffany L

    2015-01-01

    Previous studies have highlighted the antimicrobial activity of caffeine, both individually and in combination with other compounds. A proposed mechanism for caffeine's antimicrobial effects is inhibition of bacterial DNA repair pathways. The current study examines the influence of sub-lethal caffeine levels on the growth and morphology of SOS response pathway mutants of Escherichia coli. Growth inhibition after treatment with caffeine and methyl methane sulfonate (MMS), a mutagenic agent, was determined for E. coli mutants lacking key genes in the SOS response pathway. The persistence of caffeine's effects was explored by examining growth and morphology of caffeine and MMS-treated bacterial isolates in the absence of selective pressure. Caffeine significantly reduced growth of E. coli recA- and uvrA-mutants treated with MMS. However, there was no significant difference in growth between umuC-isolates treated with MMS alone and MMS in combination with caffeine after 48 h of incubation. When recA-isolates from each treatment group were grown in untreated medium, bacterial isolates that had been exposed to MMS or MMS with caffeine showed increased growth relative to controls and caffeine-treated isolates. Morphologically, recA-isolates that had been treated with caffeine and both caffeine and MMS together had begun to display filamentous growth. Caffeine treatment further reduced growth of recA- and uvrA-mutants treated with MMS, despite a non-functional SOS response pathway. However, addition of caffeine had very little effect on MMS inhibition of umuC-mutants. Thus, growth inhibition of E. coli with caffeine treatment may be driven by caffeine interaction with UmuC, but also appears to induce damage by additional mechanisms as evidenced by the additive effects of caffeine in recA- and uvrA-mutants.

  6. Functional characterization of two SOS-regulated genes involved in mitomycin C resistance in Caulobacter crescentus.

    Science.gov (United States)

    Lopes-Kulishev, Carina O; Alves, Ingrid R; Valencia, Estela Y; Pidhirnyj, María I; Fernández-Silva, Frank S; Rodrigues, Ticiane R; Guzzo, Cristiane R; Galhardo, Rodrigo S

    2015-09-01

    The SOS response is a universal bacterial regulon involved in the cellular response to DNA damage and other forms of stress. In Caulobacter crescentus, previous work has identified a plethora of genes that are part of the SOS regulon, but the biological roles of several of them remain to be determined. In this study, we report that two genes, hereafter named mmcA and mmcB, are involved in the defense against DNA damage caused by mitomycin C (MMC), but not against lesions induced by other common DNA damaging agents, such as UVC light, methyl methanesulfonate (MMS) and hydrogen peroxide. mmcA is a conserved gene that encodes a member of the glyoxalases/dioxygenases protein family, and acts independently of known DNA repair pathways. On the other hand, epistasis analysis showed that mmcB acts in the same pathway as imuC (dnaE2), and is required specifically for MMC-induced mutagenesis, but not for that induced by UV light, suggesting a role for MmcB in translesion synthesis-dependent repair of MMC damage. We show that the lack of MMC-induced mutability in the mmcB strain is not caused by lack of proper SOS induction of the imuABC operon, involved in translesion synthesis (TLS) in C. crescentus. Based on this data and on structural analysis of a close homolog, we propose that MmcB is an endonuclease which creates substrates for ImuABC-mediated TLS patches. Copyright © 2015 Elsevier B.V. All rights reserved.

  7. Leptospira interrogans serovar copenhageni harbors two lexA genes involved in SOS response.

    Directory of Open Access Journals (Sweden)

    Luciane S Fonseca

    Full Text Available Bacteria activate a regulatory network in response to the challenges imposed by DNA damage to genetic material, known as the SOS response. This system is regulated by the RecA recombinase and by the transcriptional repressor lexA. Leptospira interrogans is a pathogen capable of surviving in the environment for weeks, being exposed to a great variety of stress agents and yet retaining its ability to infect the host. This study aims to investigate the behavior of L. interrogans serovar Copenhageni after the stress induced by DNA damage. We show that L. interrogans serovar Copenhageni genome contains two genes encoding putative LexA proteins (lexA1 and lexA2 one of them being potentially acquired by lateral gene transfer. Both genes are induced after DNA damage, but the steady state levels of both LexA proteins drop, probably due to auto-proteolytic activity triggered in this condition. In addition, seven other genes were up-regulated following UV-C irradiation, recA, recN, dinP, and four genes encoding hypothetical proteins. This set of genes is potentially regulated by LexA1, as it showed binding to their promoter regions. All these regions contain degenerated sequences in relation to the previously described SOS box, TTTGN 5CAAA. On the other hand, LexA2 was able to bind to the palindrome TTGTAN10TACAA, found in its own promoter region, but not in the others. Therefore, the L. interrogans serovar Copenhageni SOS regulon may be even more complex, as a result of LexA1 and LexA2 binding to divergent motifs. New possibilities for DNA damage response in Leptospira are expected, with potential influence in other biological responses such as virulence.

  8. Efeito de Hypericum caprifoliatum Cham. & Schltdl. (Guttiferae sobre contrações em íleo isolado de cobaio induzidas por diferentes agonistas

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    Alice F. Viana

    Full Text Available Na última década, o gênero Hypericum ganhou repercussão mundial devido à utilização de Hypericum perforatum para obtenção de medicamentos antidepressivos. Por esta razão, a maioria dos estudos com outras espécies do gênero centra-se nesta atividade. Porém, um dos usos populares de espécies de Hypericum nativas do sul do Brasil é no tratamento de problemas gastrintestinais, inclusive como antiespasmódico. Neste trabalho, foi avaliado o efeito de uma das espécies de Hypericum nativas do Rio Grande do Sul, H. caprifoliatum, sobre as contrações induzidas por agonistas em íleo isolado de cobaio. Foi investigado o efeito de um extrato ciclo-hexano purificado (isento de clorofila e ceras, nas concentrações de 1, 3, 10 e 30 mg/mL, sobre curvas cumulativas de acetilcolina, histamina, potássio e serotonina (10-7 a 10-4 M. Na concentração de 30 mg/mL o extrato inibiu totalmente as contrações induzidas por todos os agonistas. Na concentração de 10 mg/mL, o extrato apresentou efeito antagonista não-competitivo de serotonina, reduzindo a contração máxima induzida por serotonina em cerca de 50 %. A resposta contrátil aos outros mediadores não foi alterada. Estes resultados indicam que espécies de Hypericum do sul do Brasil podem ser uma perspectiva interessante na busca de moléculas com atividade sobre a motilidade gastrintestinal.

  9. Análise clínica e morfológica da conjuntivite alérgica induzida por ovalbumina e tratada com chalcona em cobaias

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    Albuquerque Rosemary Jorge de Mendonça

    2004-01-01

    Full Text Available OBJETIVO: Verificar, do ponto de vista clínico e morfológico, o efeito da chalcona na conjuntivite alérgica induzida por ovalbumina em cobaias. MÉTODOS: Utilizaram-se 54 cobaias, albinos, machos pesando aproximadamente 400 g. Os animais foram sensibilizados por injeção intraperitoneal de ovalbumina suspensa em solução adjuvante completa de Freund. Posteriormente, a conjuntivite alérgica foi induzida por instilação de ovalbumina no saco conjuntival do olho direito. Os animais foram distribuídos em 3 grupos, conforme o tratamento proposto: chalcona, corticóide e salina. A avaliação clínica foi realizada com 5, 10 e 40 min, 7 e 24 h da indução e diariamente até o dia 7 da indução. A avaliação morfológica consistiu em avaliar edema, necrose, vascularização e exocitose nos dias 1, 3 e 7 da indução. RESULTADOS: Em todos os grupos a resposta inflamatória foi mais intensa 24 h após a indução. No grupo chalcona evidenciou-se menos sinais inflamatórios que no grupo salina. O grupo corticóide apresentou menos sinais inflamatórios quando comparados aos grupos chalcona e controle. À análise morfológica evidenciou-se que os grupos chalcona e corticóide apresentaram efeitos terapêuticos semelhantes. CONCLUSÃO: A chalcona teve efeito terapêutico na conjuntivite alérgica induzida por ovalbumina.

  10. Influence of the gene xthA in the activation of SOS response of Escherichia coli; Influencia del gen xthA en la activacion de la respuesta SOS de Escherichia coli

    Energy Technology Data Exchange (ETDEWEB)

    Dominguez M, V.

    2013-07-01

    The SOS response is one of the strategies that has Escherichia coli to counteract the lesions in the genetic material. The response is integrated for approximately 60 genes that when are activated they provide to the cell a bigger opportunity to survive. For the activation of this system is necessary that DNA regions of simple chain are generated, in such a way that most of the lesions should be processed, to be able to induce this answer. Some genes that intervene in this procedure, as recO, recB and recJ are recognized since when being exposed to the radiation, their activity SOS is smaller than in a wild strain. In previous works has been studied that to inactivate the genes that are involves in the lesions processing to generate DNA of simple chain, the SOS induction level diminishes with regard to a wild strain, but that when eliminating the genes that are involves directly in the repair, the SOS response increases. In this work a strain with defects in the gene xthA was built, which encodes for an endonuclease AP that participates in the repair mechanism by base excision and was evaluated their sensibility as the activity of the SOS response when exposing it to UV light and gamma radiation. The results showed that the lethality of the strain with the defect is very similar to the wild strain; while the activation level of the SOS response is bigger in comparison with the wild strain when being exposed to UV light; suggesting the existence of an enzyme that recognizes the lesions that produces this radiation, however, is not this the main repair channel, since the survival is similar to that of the wild strain. On the contrary, the results obtained with gamma radiation showed that the lethality diminishes in comparison to that of the wild strain, like the SOS activity; due surely to that the gene product intervenes in the repair for base excision, participating in the formation of the previous substrate to the activation of the SOS response. (Author)

  11. Apoptosis-like death, an extreme SOS response in Escherichia coli.

    Science.gov (United States)

    Erental, Ariel; Kalderon, Ziva; Saada, Ann; Smith, Yoav; Engelberg-Kulka, Hanna

    2014-07-15

    In bacteria, SOS is a global response to DNA damage, mediated by the recA-lexA genes, resulting in cell cycle arrest, DNA repair, and mutagenesis. Previously, we reported that Escherichia coli responds to DNA damage via another recA-lexA-mediated pathway resulting in programmed cell death (PCD). We called it apoptosis-like death (ALD) because it is characterized by membrane depolarization and DNA fragmentation, which are hallmarks of eukaryotic mitochondrial apoptosis. Here, we show that ALD is an extreme SOS response that occurs only under conditions of severe DNA damage. Furthermore, we found that ALD is characterized by additional hallmarks of eukaryotic mitochondrial apoptosis, including (i) rRNA degradation by the endoribonuclease YbeY, (ii) upregulation of a unique set of genes that we called extensive-damage-induced (Edin) genes, (iii) a decrease in the activities of complexes I and II of the electron transport chain, and (iv) the formation of high levels of OH˙ through the Fenton reaction, eventually resulting in cell death. Our genetic and molecular studies on ALD provide additional insight for the evolution of mitochondria and the apoptotic pathway in eukaryotes. Importance: The SOS response is the first described and the most studied bacterial response to DNA damage. It is mediated by a set of two genes, recA-lexA, and it results in DNA repair and thereby in the survival of the bacterial culture. We have shown that Escherichia coli responds to DNA damage by an additional recA-lexA-mediated pathway resulting in an apoptosis-like death (ALD). Apoptosis is a mode of cell death that has previously been reported only in eukaryotes. We found that E. coli ALD is characterized by several hallmarks of eukaryotic mitochondrial apoptosis. Altogether, our results revealed that recA-lexA is a DNA damage response coordinator that permits two opposite responses: life, mediated by the SOS, and death, mediated by the ALD. The choice seems to be a function of the degree

  12. Test CMOS/SOS RAM for transient radiation upset comparative research and failure analysis

    International Nuclear Information System (INIS)

    Nikiforov, A.Y.; Poljakov, I.V.

    1995-01-01

    The test Complementary Metal-Oxide-Semiconductor/Silicon-on-Sapphire Random Access Memory (CMOS/SOS RAM) with eight types of memory cells was designed and tested at high dose rates with a flash X-ray machine and laser simulator. The memory cell (MC) design with additional transistors and RC-chain was found to be upset free up to 2 x 10 12 rad(Si)/s. An inversion effect was discovered in which almost 100% logic upset was observed in poorly protected memory cell arrays at very high dose rates

  13. Evaluating the management strategies of a forestland estate--the S-O-S approach.

    Science.gov (United States)

    Kangas, Jyrki; Kurttila, Mikko; Kajanus, Miika; Kangas, Annika

    2003-12-01

    Connecting Multiple Criteria Decision Support (MCDS) methods with SWOT (Strengths, Weaknesses, Opportunities, and Threats) analysis yields analytical priorities for the factors included in SWOT analysis and makes them commensurable. In addition, decision alternatives can be evaluated with respect to each SWOT factor. In this way, SWOT analysis provides the basic frame within which to perform analyses of decision situations. MCDS methods, in turn, assist in carrying out SWOT more analytically and in elaborating the results of the analyses so that alternative strategic decisions can be prioritized also with respect to the entire SWOT. The A'WOT analysis is an example of such hybrid methods. It makes combined use of the Analytic Hierarchy Process (AHP) and SWOT. In this study, a hybrid method of the Stochastic Multicriteria Acceptability Analysis with Ordinal criteria (SMAA-O) and SWOT is developed as an elaboration of the basic ideas of A'WOT. The method is called S-O-S (SMAA-O in SWOT). SMAA-O enables the handling of ordinal preference information as well as mixed data consisting of both ordinal and cardinal information. Using SMAA-O is enough to just rank decision elements instead of giving them cardinal preference or priority ratios as required by the most commonly used MCDS methods. Using SMAA-O, in addition to analyzing what the recommended action is under certain priorities of the criteria, enables one to analyze what kind of preferences would support each action. The S-O-S approach is illustrated by a case study, where the shareholders of a forest holding owned by a private partnership prepared the SWOT analysis. Six alternative strategies for the management of their forest holding and of old cottage located on the holding were formed. After S-O-S analyses were carried out, one alternative was found to be the most recommendable. However, different importance orders of the SWOT groups would lead to different recommendations, since three of the six alternatives

  14. Regulated expression of the dinR and recA genes during competence development and SOS induction in Bacillus subtilis

    NARCIS (Netherlands)

    Haijema, BJ; vanSinderen, D; Winterling, K; Kooistra, J; Venema, G; Hamoen, LW

    1996-01-01

    It has been hypothesized that the dinR gene product of Bacillus subtilis acts as a repressor of the SOS regulon by binding to DNA sequences located upstream of SOS genes, including dinR and recA. Following activation as a result of DNA damage, RecA is believed to catalyse DinR-autocleavage, thus

  15. Influência da heparina sódica na ocorrência de laminite eqüina induzida por sobrecarga de carboidratos

    OpenAIRE

    Martins Filho,L.P.; Fagliari,J.J.; Moraes,J.R.E.; Sampaio,R.C.; Oliveira,J.A.; Lacerda Neto,J.C.

    2008-01-01

    Avaliou-se a eficiência da infusão intravenosa de heparina sódica (100UI/kg/8h, a partir de 24h após o fornecimento de carboidrato, até completar 48h) no controle da laminite eqüina experimentalmente induzida por sobrecarga de carboidrato (17,6g de amido de milho/kg de peso corpóreo). Foram utilizados 15 eqüinos adultos, distribuídos em três grupos experimentais: GI (grupo-controle); GII (grupo laminite) e GIII (grupo laminite+heparina). Posteriormente ao fornecimento de carboidrato, os anima...

  16. Lex marks the spot: the virulent side of SOS and a closer look at the LexA regulon.

    Science.gov (United States)

    Kelley, William L

    2006-12-01

    The SOS response that responds to DNA damage induces many genes that are under LexA repression. A detailed examination of LexA regulons using genome-wide techniques has recently been undertaken in both Escherichia coli and Bacillus subtilis. These extensive and elegant studies have now charted the extent of the LexA regulons, uncovered many new genes, and exposed a limited overlap in the LexA regulon between the two bacteria. As more bacterial genomes are analysed, more curiosities in LexA regulons arise. Several notable examples include the discovery of a LexA-like protein, HdiR, in Lactococcus lactis, organisms with two lexA genes, and small DNA damage-inducible cassettes under LexA control. In the cyanobacterium Synechocystis, genetic and microarray studies demonstrated that a LexA paralogue exerts control over an entirely different set of carbon-controlled genes and is crucial to cells facing carbon starvation. An examination of SOS induction evoked by common therapeutic drugs has shed new light on unsuspected consequences of drug exposure. Certain antibiotics, most notably fluoroquinolones such as ciprofloxacin, can induce an SOS response and can modulate the spread of virulence factors and drug resistance. SOS induction by beta-lactams in E. coli triggers a novel form of antibiotic defence that involves cell wall stress and signal transduction by the DpiAB two-component system. In this review, we provide an overview of these new directions in SOS and LexA research with emphasis on a few themes: identification of genes under LexA control, the identification of new endogenous triggers, and antibiotic-induced SOS response and its consequences.

  17. Tratamento farmacológico de acatisia induzida por antipsicóticos Pharmacological treatment of neuroleptic-induced akathisia

    Directory of Open Access Journals (Sweden)

    Adriano Resende Lima

    2001-06-01

    Full Text Available INTRODUÇÃO: A acatisia induzida por antipsicóticos é um transtorno de movimento relacionado ao sistema motor e caracterizado por sensação subjetiva de inquietude interna, irritabilidade ou disforia que podem ser intensas. Associa-se à sensação física e objetiva de desassossego e a movimentos não discinéticos. Esse efeito adverso pode predispor à pobre adesão ao tratamento e, conseqüentemente, favorecer recaídas. Assim, a escolha do melhor tratamento contribui para o alívio do sofrimento dos pacientes e favorece o melhor prognóstico. OBJETIVOS: Avaliar a eficácia e a tolerabilidade dos betabloqueadores, benzodiazepínicos e anticolinérgicos, comparados ao placebo e entre si, no tratamento de acatisia induzida por antipsicóticos, independente de idade ou diagnóstico psiquiátrico. MÉTODOS: Todos os estudos controlados, obtidos a partir das estratégias de busca que compararam betabloqueadores de ação central, benzodiazepínicos ou anticolinérgicos ao placebo e entre si, independente de sexo, idade ou diagnóstico psiquiátrico, foram considerados para esta atualização. Até março de 1999, foram consultadas sete bases de dados eletrônicos, sendo também examinadas as referências bibliográficas dos artigos selecionados. RESULTADOS: São apresentados 22 estudos controlados, sendo 13 comparando betabloqueadores, benzodiazepínicos e anticolinérgicos ao placebo e nove comparando diretamente as intervenções entre si. CONCLUSÕES: As três intervenções farmacológicas demonstraram eficácia superior comparadas ao placebo. Betabloqueadores de ação central mostraram-se mais eficazes, quando comparados a betabloqueadores de ação periférica, benzodiazepínicos e anticolinérgicos.INTRODUCTION: Neuroleptic-induced akathisia is a movement disorder related to the motor system characterized by complaints of inner restlessness, mental uneasiness, or dysphoria, all of which can be intense. Restlessness and non

  18. Kinetic and dose dependences of the SOS-induction in E.coli K-12 (uvrA) cells exposed to the different UV doses

    International Nuclear Information System (INIS)

    Komova, O.V.; Kandiano, E.S.; Malavya, G.

    1999-01-01

    The kinetic and dose dependences of the SOS-induction in E.coli (uvrA) cells exposed to UV light were investigated. Below 2 J/m 2 the rate of the SOS-induction increased with dose. The maximal level of the SOS-response was proportional to the UV dose. Pyrimidine dimers were necessary for the induction. In the dose range 2-10 J/m 2 the rate of the SOS-induction decreased with dose. The dose-response curve was non-linear. Pyrimidine dimers were not required for the induction. The nature of the molecular events leading to the SOS-induction at low and high UV doses was discussed. (author)

  19. Kinetic and dose dependencies of the SOS-induction in E.coli K-12 (uvrA) cells exposed to different UV doses

    International Nuclear Information System (INIS)

    Komova, O.V.; Kandiano, E.S.; Malavina, G.; )

    2000-01-01

    Kinetic and dose dependencies of the SOS-induction in E. coli (uvrA) cells exposed to UV light were investigated. below 2 J/m 2 the rate of the SOS-induction increased with dose. Maximal level of the SOS-response was proportional to the UV dose. Pyrimidine dimers were necessary for the induction. In the dose range 2-10 J/m 2 the rate of SOS-induction decreased with dose. Dose-maximum response curve was non-linear. Pyrimidine dimers were not required for the induction. nature of the molecular events leading to the SOS-induction at low and high doses was discussed [ru

  20. Coronary artery ectasia in Noonan syndrome: Report of an individual with SOS1 mutation and literature review.

    Science.gov (United States)

    Calcagni, Giulio; Baban, Anwar; De Luca, Enrica; Leonardi, Benedetta; Pongiglione, Giacomo; Digilio, Maria Cristina

    2016-03-01

    Noonan syndrome (NS) is the second most frequent hereditary syndrome with cardiac involvement. Pulmonary valve stenosis and hypertrophic cardiomyopathy are the most prevalent cardiovascular abnormalities. We report on a 14-year-old girl with NS due to SOS1 mutation with pulmonary stenosis and idiopathic coronary ectasia. To the best of our knowledge, this is the first report describing coronary ectasia in a patient with NS secondary to a SOS1 mutation. We include a literature review of this rare association. © 2015 Wiley Periodicals, Inc.

  1. ESTUDO BIOQUÍMICO DO POTENCIAL DA MEMBRANA AMNIÓTICA NA FIBROSE HEPÁTICA INDUZIDA EM RATOS

    Directory of Open Access Journals (Sweden)

    Shaine Silva Maciel

    2016-11-01

    Full Text Available A fibrose hepática causada por obstrução do ducto biliar induz alterações tanto na estrutura como na função do fígado, e pode levar a cirrose e insuficiência hepática, quando não tradada. A membrana amniótica humana (MA devido às suas propriedades anti-inflamatórias e anti-fibróticas pode ser uma terapia alternativa. A proposta desse trabalho é analisar as alterações nos níveis séricos das principais enzimas celulares e da albumina, na fibrose hepática induzida pela ligadura do ducto biliar (LDB e após o tratamento com a MA. Dez ratos foram divididos nos grupos LDB e LDB+MA, e eutanasiados após 9 semanas da LDB. As amostras de sangue foram processadas bioquimicamente para análise da albumina (ALB, fosfatase alcalina (FA e transaminases (TGP e TGO.  Comparando os grupos experimentais verificou-se que no grupo LDB+MA houve a diminuição da ALB e TGP e no grupo LDB houve a diminuição da FA e TGO, entretanto sem apresentar diferença significante entre os grupos. Conclui-se que a MA, quando aplicada ao fígado no mesmo momento da indução da fibrose, parece não ter exercido efeito significativo na função hepática.

  2. Gene Expression in Class 2 Integrons Is SOS-Independent and Involves Two Pc Promoters.

    Science.gov (United States)

    Jové, Thomas; Da Re, Sandra; Tabesse, Aurore; Gassama-Sow, Amy; Ploy, Marie-Cécile

    2017-01-01

    Integrons are powerful bacterial genetic elements that permit the expression and dissemination of antibiotic-resistance gene cassettes. They contain a promoter Pc that allows the expression of gene cassettes captured through site-specific recombination catalyzed by IntI, the integron-encoded integrase. Class 1 and 2 integrons are found in both clinical and environmental settings. The regulation of intI and of Pc promoters has been extensively studied in class 1 integrons and the regulatory role of the SOS response on intI expression has been shown. Here we investigated class 2 integrons. We characterized the P intI2 promoter and showed that intI2 expression is not regulated via the SOS response. We also showed that, unlike class 1 integrons, class 2 integrons possess not one but two active Pc promoters that are located within the attI2 region that seem to contribute equally to gene cassette expression. Class 2 integrons mostly encode an inactive truncated integrase, but the rare class 2 integrons that encode an active integrase are associated with less efficient Pc2 promoter variants. We propose an evolutionary model for class 2 integrons in which the absence of repression of the integrase gene expression led to mutations resulting in either inactive integrase or Pc variants of weaker activity, thereby reducing the potential fitness cost of these integrons.

  3. The recX gene product is involved in the SOS response in Herbaspirillum seropedicae

    International Nuclear Information System (INIS)

    Galvao, C.W.; Pedrosa, F.O.; Souza, E.M.; Yates, M.G.; Chubatsu, L.S.; Steffens, M.B.R.

    2003-01-01

    The recA and the recX genes of Herbaspirillum seropedicae were sequenced. The recX is located 359 bp downstream from recA. Sequence analysis indicated the presence of a putative operator site overlapping a probable σ 70 -dependent promoter upstream of recA and a transcription terminator downstream from recX, with no apparent promoter sequence in the intergenic region. Transcriptional analysis using lacZ promoter fusions indicated that recA expression increased three- to fourfold in the presence of methyl methanesulfonate (MMS). The roles of recA and recX genes in the SOS response were determined from studies of chromosomal mutants. The recA mutant showed the highest sensitivity to MMS and UV, and the recX mutant had an intermediate sensitivity, compared with the wild type (SMR1), confirming the essential role of the RecA protein in cell viability in the presence of mutagenic agents and also indicating a role for RecX in the SOS response. (author)

  4. The success of opening single chronic total occlusion lesions to improve myocardialviabilitytrial (SOS-COMEDY)

    Science.gov (United States)

    Huang, Rongchong; Song, Xiantao; Zhang, Haishan; Tian, Wen; Huang, Zheng; Zhang, Xingwei; Yang, Junqing; Zhang, Dongfeng; Wu, Jian; Zhong, Lei; Ting, Henry H.

    2018-01-01

    Abstract Aims: Success of opening single (SOS)-comedy is a prospective multicenter study to compare the improvement in the decrease of myocardial viability by percutaneous coronary intervention (PCI) with that by optimal medical therapy (OMT) alone in patients with chronic total occlusion (CTO) of a single coronary artery. Methods and results: The risks and the benefits of both options (PCI and OMT) were listed in a CTO decision aid (DA). Eligible participants detected by invasive coronary angiography (ICA) or coronary computed tomography angiography (CCTA) were divided into PCI or OMT groups according to patients’ choice after shared-decision making process with DA. Participants will undergo positron emission tomography/computed tomography (PET/CT), cardiac magnetic resonance (CMR) and transthoracic echocardiography (TTE), and proceed to ICA and revascularization if possible. Blinded core laboratory interpretation will be performed for ICA, CCTA, PET/CT, CMR, and TTE. All participants will be followed up for 12 months. The primary endpoint is the improvement to the decrease of myocardial viability from baseline assessed with the use of PET/CT after 12-month follow-up. Conclusions: All of the patients are appropriately consented before enrolling in this study, which has been approved by the Ethics Committee. Results of SOS-COMEDY will be helpful to develop a strategy for single CTO patients. PMID:29668609

  5. The role of the bacterial mismatch repair system in SOS-induced mutagenesis: a theoretical background

    International Nuclear Information System (INIS)

    Belov, O.V.; Kapralov, M.I.; Chuluunbaatar, O.; Sweilam, N.H.

    2012-01-01

    A theoretical study is performed of the possible role of the methyl-directed mismatch repair system in the ultraviolet-induced mutagenesis of Escherichia coli bacterial cells. For this purpose, a mathematical model of the bacterial mismatch repair system is developed. Within this model, the key pathways of this type of repair are simulated on the basis of modern experimental data related to its mechanisms. Here we have modelled in detail five main pathways of DNA misincorporation removal with different DNA exonucleases. Using our calculations, we have tested the hypothesis that the bacterial mismatch repair system is responsible for the removal of the nucleotides misincorporated by DNA polymerase V (the UmuD' 2 C complex) during ultraviolet-induced SOS response. For the theoretical analysis of the mutation frequency, we have combined the proposed mathematical approach with the model of SOS-induced mutagenesis in the E.coli bacterial cell developed earlier. Our calculations support the hypothesis that methyl-directed mismatch repair influences the mutagenic effect of ultraviolet radiation

  6. The recX gene product is involved in the SOS response in Herbaspirillum seropedicae

    Energy Technology Data Exchange (ETDEWEB)

    Galvao, C.W.; Pedrosa, F.O.; Souza, E.M.; Yates, M.G.; Chubatsu, L.S.; Steffens, M.B.R. [Univ. Federal do Parana, Dept. of Biochemistry and Molecular Biology, Curitiba (Brazil)]. E-mail: steffens@bioufpr.br

    2003-02-15

    The recA and the recX genes of Herbaspirillum seropedicae were sequenced. The recX is located 359 bp downstream from recA. Sequence analysis indicated the presence of a putative operator site overlapping a probable {sigma}{sup 70}-dependent promoter upstream of recA and a transcription terminator downstream from recX, with no apparent promoter sequence in the intergenic region. Transcriptional analysis using lacZ promoter fusions indicated that recA expression increased three- to fourfold in the presence of methyl methanesulfonate (MMS). The roles of recA and recX genes in the SOS response were determined from studies of chromosomal mutants. The recA mutant showed the highest sensitivity to MMS and UV, and the recX mutant had an intermediate sensitivity, compared with the wild type (SMR1), confirming the essential role of the RecA protein in cell viability in the presence of mutagenic agents and also indicating a role for RecX in the SOS response. (author)

  7. The recX gene product is involved in the SOS response in Herbaspirillum seropedicae.

    Science.gov (United States)

    Galvão, Carolina W; Pedrosa, Fábio O; Souza, Emanuel M; Yates, M Geoffrey; Chubatsu, Leda S; Steffens, Maria Berenice R

    2003-02-01

    The recA and the recX genes of Herbaspirillum seropedicae were sequenced. The recX is located 359 bp downstream from recA. Sequence analysis indicated the presence of a putative operator site overlapping a probable sigma70-dependent promoter upstream of recA and a transcription terminator downstream from recX, with no apparent promoter sequence in the intergenic region. Transcriptional analysis using lacZ promoter fusions indicated that recA expression increased three- to fourfold in the presence of methyl methanesulfonate (MMS). The roles of recA and recX genes in the SOS response were determined from studies of chromosomal mutants. The recA mutant showed the highest sensitivity to MMS and UV, and the recX mutant had an intermediate sensitivity, compared with the wild type (SMR1), confirming the essential role of the RecA protein in cell viability in the presence of mutagenic agents and also indicating a role for RecX in the SOS response.

  8. Structural landscape of the proline-rich domain of Sos1 nucleotide exchange factor.

    Science.gov (United States)

    McDonald, Caleb B; Bhat, Vikas; Kurouski, Dmitry; Mikles, David C; Deegan, Brian J; Seldeen, Kenneth L; Lednev, Igor K; Farooq, Amjad

    2013-01-01

    Despite its key role in mediating a plethora of cellular signaling cascades pertinent to health and disease, little is known about the structural landscape of the proline-rich (PR) domain of Sos1 guanine nucleotide exchange factor. Herein, using a battery of biophysical tools, we provide evidence that the PR domain of Sos1 is structurally disordered and adopts an extended random coil-like conformation in solution. Of particular interest is the observation that while chemical denaturation of PR domain results in the formation of a significant amount of polyproline II (PPII) helices, it has little or negligible effect on its overall size as measured by its hydrodynamic radius. Our data also show that the PR domain displays a highly dynamic conformational basin in agreement with the knowledge that the intrinsically unstructured proteins rapidly interconvert between an ensemble of conformations. Collectively, our study provides new insights into the conformational equilibrium of a key signaling molecule with important consequences on its physiological function. Copyright © 2013 Elsevier B.V. All rights reserved.

  9. Efeitos da quercetina na lesão pulmonar induzida por bleomicina: um estudo preliminar Effects of quercetin on bleomycin-induced lung injury: a preliminary study

    Directory of Open Access Journals (Sweden)

    José Antônio Baddini Martinez

    2008-07-01

    Full Text Available OBJETIVO: Investigar os efeitos da quercetina em um modelo de inflamação pulmonar e fibrose induzidas por bleomicina. MÉTODOS: Setenta e nove hamsters machos adultos foram randomizados para aplicação de injeções pelas vias intratraqueal (IT e intraperitoneal (IP em quatro configurações: veículo IP/salina IT (grupo VS, n = 16; salina IT/quercetina IP (grupo QS, n = 16; bleomicina IT/veículo IP (grupo VB, n = 27; e bleomicina IT/quercetina IP (grupo QB, n = 20. A quercetina e a bleomicina foram aplicadas em doses de 30 mg/kg/dia e 10 U/kg, respectivamente.A quercetina foi iniciada/suspensa 3 dias antes/14 dias depois das injeções IT. RESULTADOS: A taxa de mortalidade do grupo VB foi significantemente superior à dos demais grupos (44% vs. VS: 0%; QS: 0%; QB: 15%. O grupo VB exibiu níveis pulmonares de substâncias reativas ao ácido tiobarbitúrico (× 10-2 nmol/mg significativamente maiores (6,6 ± 1,3 vs. VS: 5,5 ± 0,8; QS: 2,5 ± 0,6; e QB: 5,8 ± 0,6.Os grupos VB/QB mostraram níveis pulmonares de glutationa reduzida (× 10-2 nmol/mg significativamente menores que os dos grupos VS/QS (28,9 ± 13,8/28,6 ± 14,8 vs. 43,9 ± 16,0/51,1 ± 20,3 e níveis de hidroxiprolina (mg/g significativamente maiores (201,6 ± 37,3/177,6 ± 20,3 vs. 109,6 ± 26,1/117,5 ± 32,0. CONCLUSÕES: Em um modelo animal de lesão pulmonar, a quercetina exibiu efeitos antiinflamatórios que são relacionados, pelo menos em parte, a suas propriedades antioxidantes.OBJECTIVE: The aim of this study was to identify the best experimental model in which to observe the pulmonary alterations characterizing hepatopulmonary syndrome (HPS. METHODS: Male Wistar rats, with mean weight of 250 g, were used in four experimental models: inhaled carbon tetrachloride; intraperitoneal carbon tetrachloride; partial portal vein ligation; and bile duct ligation (BDL. The animals in all groups were divided into control and experimental subgroups. The following variables were

  10. SOS! Ayuda para Padres: Una Guia Practica para Manejar Problemas de Conducta Comunes y Corrientes. (SOS! Help for Parents: A Practical Guide for Handling Common Everyday Behavior Problems.) Leader's Guide.

    Science.gov (United States)

    Clark, Lynn

    This Spanish-language version of "SOS" provides parents with guidance for handling a variety of common behavior problems based on the behavior approach to child rearing and discipline. This approach suggests that good and bad behavior are both learned and can be changed, and proposes specific methods, skills, procedures, and strategies…

  11. Quantification, modelling and design for signal history dependent effects in mixed-signal SOI/SOS circuits; Quantification, modelisation et conception prenant en compte les etats anterieurs des signaux dans les circuits mixtes SOI/SOS

    Energy Technology Data Exchange (ETDEWEB)

    Edwards, C.F.; Redman-White, W.; Bracey, M.; Tenbroek, B.M.; Lee, M.S. [Southampton Univ., Dept. of Electronics and Computer Sciences (United Kingdom); Uren, M.J.; Brunson, K.M. [DERA Farnborough, GU, Hants (United Kingdom)

    1999-07-01

    This paper deals with how the radiation hardness of mixed signal SOI/SOS CMOS circuits is taken into account at both architectural terms as well as the the transistor level cell designs. The primary issue is to deal with divergent transistor threshold shifts, and to understand the effects of large amplitude non stationary signals on analogue cell behaviour. (authors)

  12. Ginecomastia induzida por anti-histamínicos no tratamento da urticária crônica Antihistaminic-induced gynecomastia in chronic urticaria treatment

    Directory of Open Access Journals (Sweden)

    Ana Paula Fusel de Ue

    2007-06-01

    Full Text Available Os anti-histamínicos são drogas muito usadas na prática do dermatologista, sendo a primeira escolha no tratamento da urticária crônica. Os efeitos colaterais mais comuns são os relacionados ao sistema nervoso central. A ginecomastia é decorrente de várias causas, entre elas a indução por drogas. Apresenta-se caso de ginecomastia induzida por anti-histamínico H1,em paciente em tratamento de urticária crônica. A investigação laboratorial e radiológica descartou outras causas para a ginecomastia, que involuiu com a retirada da medicação. Objetiva-se discutir os efeitos colaterais dos anti-histamínicos e apresentar caso de ginecomastia induzida por drogas.Antihistaminic drugs are very commonly used in dermatological practice, and are the first-line therapy to chronic urticaria. The commonest side effects are related to the central nervous system. Gynecomastia, in turn, may be due to a myriad of disorders, including the use of medication. The case of H1 antihistaminic-induced gynecomastia in a patient undergoing chronic urticaria treatment is reported. Radiological and laboratorial investigations discarded other possible causes for the gynecomastia, which disappeared after removal of the medication. Antihistaminic-related side effects, including gynecomastia, are discussed.

  13. Existe associação entre antiinflamatórios não-esteróides e nefropatia induzida por contraste? = Is there association between non-steroidal anti-inflammatory drugs and contrast media-induced nephropathy?

    Directory of Open Access Journals (Sweden)

    Diogo, Luciano Passamani

    2008-01-01

    Conclusões: faltam evidências, na literatura médica indexada, que sustentem a afirmação de que os antiinflamatórios não esteróides seriam fator de risco para o desenvolvimento de nefropatia induzida por contraste

  14. Activation of multiple signaling pathways causes developmental defects in mice with a Noonan syndrome–associated Sos1 mutation

    Science.gov (United States)

    Chen, Peng-Chieh; Wakimoto, Hiroko; Conner, David; Araki, Toshiyuki; Yuan, Tao; Roberts, Amy; Seidman, Christine E.; Bronson, Roderick; Neel, Benjamin G.; Seidman, Jonathan G.; Kucherlapati, Raju

    2010-01-01

    Noonan syndrome (NS) is an autosomal dominant genetic disorder characterized by short stature, unique facial features, and congenital heart disease. About 10%–15% of individuals with NS have mutations in son of sevenless 1 (SOS1), which encodes a RAS and RAC guanine nucleotide exchange factor (GEF). To understand the role of SOS1 in the pathogenesis of NS, we generated mice with the NS-associated Sos1E846K gain-of-function mutation. Both heterozygous and homozygous mutant mice showed many NS-associated phenotypes, including growth delay, distinctive facial dysmorphia, hematologic abnormalities, and cardiac defects. We found that the Ras/MAPK pathway as well as Rac and Stat3 were activated in the mutant hearts. These data provide in vivo molecular and cellular evidence that Sos1 is a GEF for Rac under physiological conditions and suggest that Rac and Stat3 activation might contribute to NS phenotypes. Furthermore, prenatal administration of a MEK inhibitor ameliorated the embryonic lethality, cardiac defects, and NS features of the homozygous mutant mice, demonstrating that this signaling pathway might represent a promising therapeutic target for NS. PMID:21041952

  15. Simultaneous Scheduling of Jobs, AGVs and Tools Considering Tool Transfer Times in Multi Machine FMS By SOS Algorithm

    Science.gov (United States)

    Sivarami Reddy, N.; Ramamurthy, D. V., Dr.; Prahlada Rao, K., Dr.

    2017-08-01

    This article addresses simultaneous scheduling of machines, AGVs and tools where machines are allowed to share the tools considering transfer times of jobs and tools between machines, to generate best optimal sequences that minimize makespan in a multi-machine Flexible Manufacturing System (FMS). Performance of FMS is expected to improve by effective utilization of its resources, by proper integration and synchronization of their scheduling. Symbiotic Organisms Search (SOS) algorithm is a potent tool which is a better alternative for solving optimization problems like scheduling and proven itself. The proposed SOS algorithm is tested on 22 job sets with makespan as objective for scheduling of machines and tools where machines are allowed to share tools without considering transfer times of jobs and tools and the results are compared with the results of existing methods. The results show that the SOS has outperformed. The same SOS algorithm is used for simultaneous scheduling of machines, AGVs and tools where machines are allowed to share tools considering transfer times of jobs and tools to determine the best optimal sequences that minimize makespan.

  16. Detection of early psychotic symptoms: Validation of the Spanish version of the "Symptom Onset in Schizophrenia (SOS) inventory".

    Science.gov (United States)

    Mezquida, Gisela; Cabrera, Bibiana; Martínez-Arán, Anabel; Vieta, Eduard; Bernardo, Miguel

    2018-03-01

    The period of subclinical signs that precedes the onset of psychosis is referred to as the prodrome or high-risk mental state. The "Symptom Onset in Schizophrenia (SOS) inventory" is an instrument to characterize and date the initial symptoms of a psychotic illness. The present study aims to provide reliability and validity data for clinical and research use of the Spanish version of the SOS. Thirty-six participants with a first-episode of psychosis meeting DSM-IV criteria for schizophrenia/schizoaffective/schizophreniform disorder were administered the translated SOS and other clinical assessments. The internal validity, intrarater and interrater reliability were studied. We found strong interrater reliability. To detect the presence/absence of prodromal symptoms, Kappa coefficients ranged between 0.8 and 0.7. Similarly, the raters obtained an excellent level of agreement regarding the onset of each symptom and the duration of symptoms until first treatment (intraclass correlation coefficients between 0.9 and 1.0). Cronbach's alpha was 0.9-1.0 for all the items. The interrater reliability and concurrent validity were also excellent in both cases. This study provides robust psychometric properties of the Spanish version of the SOS. The translated version is adequate in terms of good internal validity, intrarater and interrater reliability, and is as time-efficient as the original version. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. DNA compaction in the early part of the SOS response is dependent on RecN and RecA.

    Science.gov (United States)

    Odsbu, Ingvild; Skarstad, Kirsten

    2014-05-01

    The nucleoids of undamaged Escherichia coli cells have a characteristic shape and number, which is dependent on the growth medium. Upon induction of the SOS response by a low dose of UV irradiation an extensive reorganization of the nucleoids occurred. Two distinct phases were observed by fluorescence microscopy. First, the nucleoids were found to change shape and fuse into compact structures at midcell. The compaction of the nucleoids lasted for 10-20 min and was followed by a phase where the DNA was dispersed throughout the cells. This second phase lasted for ~1 h. The compaction was found to be dependent on the recombination proteins RecA, RecO and RecR as well as the SOS-inducible, SMC (structural maintenance of chromosomes)-like protein RecN. RecN protein is produced in high amounts during the first part of the SOS response. It is possible that the RecN-mediated 'compact DNA' stage at the beginning of the SOS response serves to stabilize damaged DNA prior to recombination and repair.

  18. The antimicrobial lysine-peptoid hybrid LP5 inhibits DNA replication and induces the SOS response in Staphylococcus aureus

    DEFF Research Database (Denmark)

    Gottschalk, Sanne; Ifrah, Dan; Lerche, Sandra

    2013-01-01

    the growth of S. aureus without ATP leakage. Instead, LP5 bound DNA and inhibited macromolecular synthesis. The binding to DNA also led to inhibition of DNA gyrase and topoisomerase IV and caused induction of the SOS response. CONCLUSIONS: Our data demonstrate that LP5 may have a dual mode of action against...

  19. Quantification, modelling and design for signal history dependent effects in mixed-signal SOI/SOS circuits

    International Nuclear Information System (INIS)

    Edwards, C.F.; Redman-White, W.; Bracey, M.; Tenbroek, B.M.; Lee, M.S.; Uren, M.J.; Brunson, K.M.

    1999-01-01

    This paper deals with how the radiation hardness of mixed signal SOI/SOS CMOS circuits is taken into account at both architectural terms as well as the the transistor level cell designs. The primary issue is to deal with divergent transistor threshold shifts, and to understand the effects of large amplitude non stationary signals on analogue cell behaviour. (authors)

  20. Identification of genes involved in low aminoglycoside-induced SOS response in Vibrio cholerae: a role for transcription stalling and Mfd helicase.

    Science.gov (United States)

    Baharoglu, Zeynep; Babosan, Anamaria; Mazel, Didier

    2014-02-01

    Sub-inhibitory concentrations (sub-MIC) of antibiotics play a very important role in selection and development of resistances. Unlike Escherichia coli, Vibrio cholerae induces its SOS response in presence of sub-MIC aminoglycosides. A role for oxidized guanine residues was observed, but the mechanisms of this induction remained unclear. To select for V. cholerae mutants that do not induce low aminoglycoside-mediated SOS induction, we developed a genetic screen that renders induction of SOS lethal. We identified genes involved in this pathway using two strategies, inactivation by transposition and gene overexpression. Interestingly, we obtained mutants inactivated for the expression of proteins known to destabilize the RNA polymerase complex. Reconstruction of the corresponding mutants confirmed their specific involvement in induction of SOS by low aminoglycoside concentrations. We propose that DNA lesions formed on aminoglycoside treatment are repaired through the formation of single-stranded DNA intermediates, inducing SOS. Inactivation of functions that dislodge RNA polymerase leads to prolonged stalling on these lesions, which hampers SOS induction and repair and reduces viability under antibiotic stress. The importance of these mechanisms is illustrated by a reduction of aminoglycoside sub-MIC. Our results point to a central role for transcription blocking at DNA lesions in SOS induction, so far underestimated.

  1. NKS1, Na+- and K+-sensitive 1, regulates ion homeostasis in an SOS-independent pathway in Arabidopsis

    KAUST Repository

    Choi, Wonkyun; Baek, Dongwon; Oh, Dongha; Park, Jiyoung; Hong, Hyewon; Kim, Woeyeon; Bohnert, Hans Jü rgen; Bressan, Ray Anthony; Park, Hyeongcheol; Yun, Daejin

    2011-01-01

    An Arabidopsis thaliana mutant, nks1-1, exhibiting enhanced sensitivity to NaCl was identified in a screen of a T-DNA insertion population in the genetic background of Col-0 gl1 sos3-1. Analysis of the genome sequence in the region flanking the T-DNA left border indicated two closely linked mutations in the gene encoded at locus At4g30996. A second allele, nks1-2, was obtained from the Arabidopsis Biological Resource Center. NKS1 mRNA was detected in all parts of wild-type plants but was not detected in plants of either mutant, indicating inactivation by the mutations. Both mutations in NKS1 were associated with increased sensitivity to NaCl and KCl, but not to LiCl or mannitol. NaCl sensitivity was associated with nks1 mutations in Arabidopsis lines expressing either wild type or alleles of SOS1, SOS2 or SOS3. The NaCl-sensitive phenotype of the nks1-2 mutant was complemented by expression of a full-length NKS1 allele from the CaMV35S promoter. When grown in medium containing NaCl, nks1 mutants accumulated more Na+ than wild type and K +/Na+ homeostasis was perturbed. It is proposed NKS1, a plant-specific gene encoding a 19 kDa endomembrane-localized protein of unknown function, is part of an ion homeostasis regulation pathway that is independent of the SOS pathway. © 2011 Elsevier Ltd. All rights reserved.

  2. Non-equilibrium repressor binding kinetics link DNA damage dose to transcriptional timing within the SOS gene network.

    Science.gov (United States)

    Culyba, Matthew J; Kubiak, Jeffrey M; Mo, Charlie Y; Goulian, Mark; Kohli, Rahul M

    2018-06-01

    Biochemical pathways are often genetically encoded as simple transcription regulation networks, where one transcription factor regulates the expression of multiple genes in a pathway. The relative timing of each promoter's activation and shut-off within the network can impact physiology. In the DNA damage repair pathway (known as the SOS response) of Escherichia coli, approximately 40 genes are regulated by the LexA repressor. After a DNA damaging event, LexA degradation triggers SOS gene transcription, which is temporally separated into subsets of 'early', 'middle', and 'late' genes. Although this feature plays an important role in regulating the SOS response, both the range of this separation and its underlying mechanism are not experimentally defined. Here we show that, at low doses of DNA damage, the timing of promoter activities is not separated. Instead, timing differences only emerge at higher levels of DNA damage and increase as a function of DNA damage dose. To understand mechanism, we derived a series of synthetic SOS gene promoters which vary in LexA-operator binding kinetics, but are otherwise identical, and then studied their activity over a large dose-range of DNA damage. In distinction to established models based on rapid equilibrium assumptions, the data best fit a kinetic model of repressor occupancy at promoters, where the drop in cellular LexA levels associated with higher doses of DNA damage leads to non-equilibrium binding kinetics of LexA at operators. Operators with slow LexA binding kinetics achieve their minimal occupancy state at later times than operators with fast binding kinetics, resulting in a time separation of peak promoter activity between genes. These data provide insight into this remarkable feature of the SOS pathway by demonstrating how a single transcription factor can be employed to control the relative timing of each gene's transcription as a function of stimulus dose.

  3. NKS1, Na+- and K+-sensitive 1, regulates ion homeostasis in an SOS-independent pathway in Arabidopsis

    KAUST Repository

    Choi, Wonkyun

    2011-04-01

    An Arabidopsis thaliana mutant, nks1-1, exhibiting enhanced sensitivity to NaCl was identified in a screen of a T-DNA insertion population in the genetic background of Col-0 gl1 sos3-1. Analysis of the genome sequence in the region flanking the T-DNA left border indicated two closely linked mutations in the gene encoded at locus At4g30996. A second allele, nks1-2, was obtained from the Arabidopsis Biological Resource Center. NKS1 mRNA was detected in all parts of wild-type plants but was not detected in plants of either mutant, indicating inactivation by the mutations. Both mutations in NKS1 were associated with increased sensitivity to NaCl and KCl, but not to LiCl or mannitol. NaCl sensitivity was associated with nks1 mutations in Arabidopsis lines expressing either wild type or alleles of SOS1, SOS2 or SOS3. The NaCl-sensitive phenotype of the nks1-2 mutant was complemented by expression of a full-length NKS1 allele from the CaMV35S promoter. When grown in medium containing NaCl, nks1 mutants accumulated more Na+ than wild type and K +/Na+ homeostasis was perturbed. It is proposed NKS1, a plant-specific gene encoding a 19 kDa endomembrane-localized protein of unknown function, is part of an ion homeostasis regulation pathway that is independent of the SOS pathway. © 2011 Elsevier Ltd. All rights reserved.

  4. Necrose cutânea induzida por antagonistas da vitamina K Skin necrosis induced by vitamin K antagonists

    Directory of Open Access Journals (Sweden)

    Jose Manoel da Silva Silvestre

    2009-12-01

    Full Text Available Os anticoagulantes orais que atuam através do antagonismo à vitamina K são utilizados na prática clínica há muito tempo, porém ainda há dificuldades no seu manejo e na condução das complicações. Entre as complicações, as mais conhecidas são os transtornos hemorrágicos, mas outras também devem ser reconhecidas, tais como a necrose induzida por varfarina. Esta é uma grave, porém rara complicação, cuja fisiopatologia é ainda obscura e cujas causas são indefinidas. Dentre as possíveis causas, as mais prováveis são a deficiência de proteína C e de proteína S, reações de hipersensibilidade e deficiência de fator VII. Há maior incidência desta complicação entre mulheres de meia-idade, acometendo preferencialmente mamas e glúteos. As medidas mais importantes para o tratamento são: suspensão imediata da droga, uso de heparina não fracionada ou de baixo peso molecular em doses terapêuticas, emprego da vitamina K e, eventualmente, infusão de plasma fresco congelado ou de proteína C ativada recombinante.Oral anticoagulants acting via vitamin K antagonists have long been employed in the clinical practice. However, difficulties related to the management of treatment regimens and complications still persist. Among the complications, bleeding disorders are widely known, but others should also be taken into consideration, such as warfarin-induced skin necrosis. The pathophysiology of this rare but severe complication is still obscure, and its causes remain to be defined. Among possible causes, protein C and protein S deficiency, hypersensitivity reactions and VII factor deficiency are the most probable ones. There is an increased incidence of warfarin-induced skin necrosis among middle-aged women, usually affecting breasts and buttocks. The most important treatment measures are immediate discontinuation of the drug, use of unfractionated or low-molecular-weight heparin at therapeutic doses, use of vitamin K and

  5. Activation of the SOS response increases the frequency of small colony variants

    DEFF Research Database (Denmark)

    Vestergaard, Martin; Paulander, Wilhelm Erik Axel; Ingmer, Hanne

    2015-01-01

    BACKGROUND: In Staphylococcus aureus sub-populations of slow-growing cells forming small colony variants (SCVs) are associated with persistent and recurrent infections that are difficult to eradicate with antibiotic therapies. In SCVs that are resistant towards aminoglycosides, mutations have been...... with different mechanism of action influence the formation of SCVs that are resistant to otherwise lethal concentrations of the aminoglycoside, gentamicin. We found that exposure of S. aureus to fluoroquinolones and mitomycin C increased the frequency of gentamicin resistant SCVs, while other antibiotic classes...... failed to do so. The higher proportion of SCVs in cultures exposed to fluoroquinolones and mitomycin C compared to un-exposed cultures correlate with an increased mutation rate monitored by rifampicin resistance and followed induction of the SOS DNA damage response. CONCLUSION: Our observations suggest...

  6. The Numerical Simulation of the Nanosecond Switching of a p-SOS Diode

    Science.gov (United States)

    Podolska, N. I.; Lyublinskiy, A. G.; Grekhov, I. V.

    2017-12-01

    Abrupt high-density reverse current interruption has been numerically simulated for switching from forward to reverse bias in a silicon p + P 0 n + structure ( p-SOS diode). It has been shown that the current interruption in this structure occurs as a result of the formation of two dynamic domains of a strong electric field in regions in which the free carrier concentration substantially exceeds the concentration of the doping impurity. The first domain is formed in the n + region at the n + P 0 junction, while the second domain is formed in the P 0 region at the interface with the p + layer. The second domain expands much faster, and this domain mainly determines the current interruption rate. Good agreement is achieved between the simulation results and the experimental data when the actual electric circuit determining the electron-hole plasma pumping in and out is accurately taken into account.

  7. Determining mode excitations of vacuum electronics devices via three-dimensional simulations using the SOS code

    Science.gov (United States)

    Warren, Gary

    1988-01-01

    The SOS code is used to compute the resonance modes (frequency-domain information) of sample devices and separately to compute the transient behavior of the same devices. A code, DOT, is created to compute appropriate dot products of the time-domain and frequency-domain results. The transient behavior of individual modes in the device is then plotted. Modes in a coupled-cavity traveling-wave tube (CCTWT) section excited beam in separate simulations are analyzed. Mode energy vs. time and mode phase vs. time are computed and it is determined whether the transient waves are forward or backward waves for each case. Finally, the hot-test mode frequencies of the CCTWT section are computed.

  8. Electrical and crystallographic evaluation of SOS implanted with silicon and/or oxygen

    International Nuclear Information System (INIS)

    Yamamoto, Y.; Kobayashi, H.; Takahashi, T.; Inada, T.

    1985-01-01

    RBS and Hall measurements have revealed that the formation of an amorphous laer in SOS near in the Si/sapphire interface by oxygen implantation at 130 K followed by regrowth by thermal annealing above 800 0 C for 20 min in N 2 is effective in improving crystalline quality and Hall mobility as well as in increasing activation of implanted P. The temperature dependence of the mobility was measured. The mobility increased by 80% and 40% at 77 K and RT, respectively, after improvement in crystalline quality. The costly low temperature implantation of O can be replaced with dual implantation of Si and O; formation of an amorphous layer by Si implantation and Al gettering by oxygen implantation. (orig.)

  9. Radiation hardness tests with a demonstrator preamplifier circuit manufactured in silicon on sapphire (SOS) VLSI technology

    International Nuclear Information System (INIS)

    Bingefors, N.; Ekeloef, T.; Eriksson, C.; Paulsson, M.; Moerk, G.; Sjoelund, A.

    1992-01-01

    Samples of the preamplifier circuit, as well as of separate n and p channel transistors of the type contained in the circuit, were irradiated with gammas from a 60 Co source up to an integrated dose of 3 Mrad (30 kGy). The VLSI manufacturing technology used is the SOS4 process of ABB Hafo. A first analysis of the tests shows that the performance of the amplifier remains practically unaffected by the radiation for total doses up to 1 Mrad. At higher doses up to 3 Mrad the circuit amplification factor decreases by a factor between 4 and 5 whereas the output noise level remains unchanged. It is argued that it may be possible to reduce the decrease in amplification factor in future by optimizing the amplifier circuit design further. (orig.)

  10. Development of the software of the data taking system SOS for the SAPHIR experiment

    International Nuclear Information System (INIS)

    Manns, J.

    1989-02-01

    The data acquistion system SOS has been developed for the SAPHIR experiment at the Bonn stretcher ring ELSA. It can handle up to 280 kilobytes of data per second or a maximum triggerrate of 200 Hz. The multiprocessor based online system consists of twenty VIP-microprocessors and two VAX-computers. Each component of the SAPHIR experiment has at least one program in the online system to maintain special functions for this specific component. All of these programs can receive event data without interfering with the transfer of events to a mass storage for offline analysis. A special program SOL has been developed to serve as a user interface to the data acquisition system and as a status display for most of the programs of the online system. Using modern features like windowing and mouse control on a VAX-station the SAPHIR online SOL establishes an easy way of controlling the data acquisition system. (orig.)

  11. A System of Systems (SoS) Approach to Sustainable Energy Planning

    Science.gov (United States)

    Madani, Kaveh; Hadian, Saeed

    2015-04-01

    The general policy of mandating fossil fuel replacement with "green" energies may not be as effective and environmental-friendly as perceived, due to the secondary impacts of renewable energies on different natural resources. An integrated systems analysis framework is essential to developing sustainable energy supply systems with minimal unintended impacts on valuable natural resources such as water, climate, and ecosystem. This presentation discusses how a system of systems (SoS) framework can be developed to quantitatively evaluate the desirability of different energy supply alternatives with respect to different sustainability criteria under uncertainty. Relative Aggregate Footprint (RAF) scores of a range of renewable and nonrenewable energy alternatives are determined using their performance values under four sustainability criteria, namely carbon footprint, water footprint, land footprint, and cost of energy production. Our results suggest that despite their lower emissions, some renewable energy sources are less promising than non-renewable energy sources from a SoS perspective that considers the trade-offs between carbon footprint of energies and their effects on water, ecosystem, and economic resources. A new framework based on the Modern Portfolio Theory (MPT) is also proposed for analyzing the overall sustainability of different energy mixes for different risk of return levels with respect to the trade-offs involved. It is discussed how the proposed finance-based sustainability evaluation method can help policy makers maximize the energy portfolio's expected sustainability for a given amount of portfolio risk, or equivalently minimize risk for a given level of expected sustainability level, by revising the energy mix.

  12. Comparison of digital tomosynthesis and computed tomography for lung nodule detection in SOS screening program.

    Science.gov (United States)

    Grosso, Maurizio; Priotto, Roberto; Ghirardo, Donatella; Talenti, Alberto; Roberto, Emanuele; Bertolaccini, Luca; Terzi, Alberto; Chauvie, Stéphane

    2017-08-01

    To compare the lung nodules' detection of digital tomosynthesis (DTS) and computed tomography (CT) in the context of the SOS (Studio OSservazionale) prospective screening program for lung cancer detection. One hundred and thirty-two of the 1843 subjects enrolled in the SOS study underwent CT because non-calcified nodules with diameters larger than 5 mm and/or multiple nodules were present in DTS. Two expert radiologists reviewed the exams classifying the nodules based on their radiological appearance and their dimension. LUNG-RADS classification was applied to compare receiver operator characteristics curve between CT and DTS with respect to final diagnosis. CT was used as gold standard. DTS and CT detected 208 and 179 nodules in the 132 subjects, respectively. Of these 208 nodules, 189 (91%) were solid, partially solid, and ground glass opacity. CT confirmed 140/189 (74%) of these nodules but found 4 nodules that were not detected by DTS. DTS and CT were concordant in 62% of the cases applying the 5-point LUNG-RADS scale. The concordance rose to 86% on a suspicious/non-suspicious binary scale. The areas under the curve in receiver operator characteristics were 0.89 (95% CI 0.83-0.94) and 0.80 (95% CI 0.72-0.89) for CT and DTS, respectively. The mean effective dose was 0.09 ± 0.04 mSv for DTS and 4.90 ± 1.20 mSv for CT. The use of a common classification for nodule detection in DTS and CT helps in comparing the two technologies. DTS detected and correctly classified 74% of the nodules seen by CT but lost 4 nodules identified by CT. Concordance between DTS and CT rose to 86% of the nodules when considering LUNG-RADS on a binary scale.

  13. Respostas cardiovasculares agudas em ambiente virtualmente simulado pelo Nintendo Wii

    Directory of Open Access Journals (Sweden)

    Renato Aparecido de Souza

    2013-01-01

    Full Text Available DOI: http://dx.doi.org/10.5007/1980-0037.2013v15n1p60 Recentemente, tem sido verificada a utilização do Nintendo Wii no contexto da saúde. O objetivo do presente estudo foi analisar as respostas cardiovasculares agudas monitoradas por meio do comportamento da frequência cardíaca, pressão arterial sistólica, pressão arterial diastólica e duplo produto, em ambiente virtualmente simulado pelo console Nintendo Wii. A amostra foi composta por 18 universitários saudáveis, com média de idade de 22,07 ± 1,34 anos. As variáveis foram observadas com uso de deltas (valor final - inicial após 25 arremessos de basquetebol em duas situações experimentais: (I com o voluntário sentado e, (II com o voluntário saltando verticalmente. Os resultados sugerem que a prática de atividade física em ambiente virtual emulado pelo Nintendo Wii é capaz de alterar as respostas cardiovasculares agudas, especialmente, quando realizada em associação a saltos verticais. Assim, os resultados sustentam a viabilidade do uso do Nintendo Wii em programas de treinamento e favorecem sua indicação de forma mais segura.

  14. SOS1 gene polymorphisms are associated with gestational diabetes mellitus in a Chinese population: Results from a nested case-control study in Taiyuan, China.

    Science.gov (United States)

    Chen, Qiong; Yang, Hailan; Feng, Yongliang; Zhang, Ping; Wu, Weiwei; Li, Shuzhen; Thompson, Brian; Wang, Xin; Peng, Tingting; Wang, Fang; Xie, Bingjie; Guo, Pengge; Li, Mei; Wang, Ying; Zhao, Nan; Wang, Suping; Zhang, Yawei

    2018-03-01

    Gestational diabetes mellitus is a growing public health concern due to its large disease burden; however, the underlying pathophysiology remains unclear. Therefore, we examined the relationship between 107 single-nucleotide polymorphisms in insulin signalling pathway genes and gestational diabetes mellitus risk using a nested case-control study. The SOS1 rs7598922 GA and AA genotype were statistically significantly associated with reduced gestational diabetes mellitus risk ( p trend  = 0.0006) compared with GG genotype. At the gene level, SOS1 was statistically significantly associated with gestational diabetes mellitus risk after adjusting for multiple comparisons. Moreover, AGGA and GGGG haplotypes in SOS1 gene were associated with reduced risk of gestational diabetes mellitus. Our study provides evidence for an association between the SOS1 gene and risk of gestational diabetes mellitus; however, its role in the pathogenesis of gestational diabetes mellitus will need to be verified by further studies.

  15. SHOPPING CENTERS: UMA RELAÇÃO ENTRE OS ATRIBUTOS DE ESCOLHA PELOS CONSUMIDORES VERSUS OS ATRIBUTOS VALORIZADOS PELOS GERENTES

    Directory of Open Access Journals (Sweden)

    Vitor Edson Marques Junior

    2010-04-01

    Full Text Available Embora os shoppings centers estejam em franco processo de ampliação na economia brasileira ainda são poucas as análises envolvendo shoppings centers e atributos motivadores de escolha pelos consumidores. Este trabalho tem por objetivo analisar os fatores motivacionais determinantes para a escolha de um shopping por parte dos consumidores. A amostra deste estudo foi composta por alunos de graduação de cursos de Administração. Adicionalmente, foi realizada uma entrevista em profundidade com os gestores dos três shoppings centers da cidade de Ribeirão Preto. O estudo revelou que os atributos motivadores mais valorizados no processo de escolha foram: limpeza, manutenção, segurança dentro do shopping e qualidade do serviço prestado. Este trabalho pretende contribuir com a compreensão da importância dos fatores valorados pelos consumidores de shoppings centers e assim sendo, fornecer material para a melhoria da gestão destes empreendimentos.

  16. Motion-insensitive carotid intraplaque hemorrhage imaging using 3D inversion recovery preparation stack of stars (IR-prep SOS) technique.

    Science.gov (United States)

    Kim, Seong-Eun; Roberts, John A; Eisenmenger, Laura B; Aldred, Booth W; Jamil, Osama; Bolster, Bradley D; Bi, Xiaoming; Parker, Dennis L; Treiman, Gerald S; McNally, J Scott

    2017-02-01

    Carotid artery imaging is important in the clinical management of patients at risk for stroke. Carotid intraplaque hemorrhage (IPH) presents an important diagnostic challenge. 3D magnetization prepared rapid acquisition gradient echo (MPRAGE) has been shown to accurately image carotid IPH; however, this sequence can be limited due to motion- and flow-related artifact. The purpose of this work was to develop and evaluate an improved 3D carotid MPRAGE sequence for IPH detection. We hypothesized that a radial-based k-space trajectory sequence such as "Stack of Stars" (SOS) incorporated with inversion recovery preparation would offer reduced motion sensitivity and more robust flow suppression by oversampling of central k-space. A total of 31 patients with carotid disease (62 carotid arteries) were imaged at 3T magnetic resonance imaging (MRI) with 3D IR-prep Cartesian and SOS sequences. Image quality was determined between SOS and Cartesian MPRAGE in 62 carotid arteries using t-tests and multivariable linear regression. Kappa analysis was used to determine interrater reliability. In all, 25 among 62 carotid plaques had carotid IPH by consensus from the reviewers on SOS compared to 24 on Cartesian sequence. Image quality was significantly higher with SOS compared to Cartesian (mean 3.74 vs. 3.11, P SOS acquisition yielded sharper image features with less motion (19.4% vs. 45.2%, P SOS (kappa = 0.89), higher than that of Cartesian (kappa = 0.84). By minimizing flow and motion artifacts and retaining high interrater reliability, the SOS MPRAGE has important advantages over Cartesian MPRAGE in carotid IPH detection. 1 J. Magn. Reson. Imaging 2017;45:410-417. © 2016 International Society for Magnetic Resonance in Medicine.

  17. Glutathione S-transferase M1-null genotype as risk factor for SOS in oxaliplatin-treated patients with metastatic colorectal cancer.

    Science.gov (United States)

    Vreuls, C P H; Olde Damink, S W M; Koek, G H; Winstanley, A; Wisse, E; Cloots, R H E; van den Broek, M A J; Dejong, C H C; Bosman, F T; Driessen, A

    2013-02-19

    Oxaliplatin is used as a neo-adjuvant therapy in hepatic colorectal carcinoma metastasis. This treatment has significant side effects, as oxaliplatin is toxic to the sinusoidal endothelial cells and can induce sinusoidal obstruction syndrome (SOS), which is related to decreased overall survival. Glutathione has an important role in the defence system, catalysed by glutathione S-transferase (GST), including two non-enzyme producing polymorphisms (GSTM1-null and GSTT1-null). We hypothesise that patients with a non-enzyme producing polymorphism have a higher risk of developing toxic injury owing to oxaliplatin. In the nontumour-bearing liver, the presence of SOS was studied histopathologically. The genotype was determined by a semi-nested PCR. Thirty-two of the 55 (58%) patients showed SOS lesions, consisting of 27% mild, 22% moderate and 9% severe lesions. The GSTM1-null genotype was present in 25 of the 55 (46%). Multivariate analysis showed that the GSTM1-null genotype significantly correlated with the presence of (moderate-severe) SOS (P=0.026). The GSTM1-null genotype is an independent risk factor for SOS. This finding allows us, in association with other risk factors, to conceive a potential risk profile predicting whether the patient is at risk of developing SOS, before starting oxaliplatin, and subsequently might result in adjustment of treatment.

  18. Infestaçao humana pelo Dipylidium caninum

    Directory of Open Access Journals (Sweden)

    Carlos Henrique Lemos

    1985-12-01

    Full Text Available Os autores descrevem um caso de infestação pelo Dipylidium caninum numa criança residente em Araguari, Minas Gerais, que apresentou lesões cutâneas, insônia, irritabilidade, vômitos eperda do apetite. Relatam a eliminação abundante deproglotes quando a paciente foi tratada com medicamentos caseiros e a remissão dos sintomas, após dois tratamentos com mebendazol. Contudo não puderam concluir sobre a eficiência das drogas, devido à medicação empírica e ã administração de 200 mg/dia de mebendazol, contrariando a dosagem recomendada para Cestodas, que é de 400 mg/dia. Relatam, ainda, que exames das fezes do cão de propriedade da família revelaram apenas a presença de ancilostomídeos.

  19. Infestaçao humana pelo Dipylidium caninum

    OpenAIRE

    Carlos Henrique Lemos; Clarice de Resende Oliveira

    1985-01-01

    Os autores descrevem um caso de infestação pelo Dipylidium caninum numa criança residente em Araguari, Minas Gerais, que apresentou lesões cutâneas, insônia, irritabilidade, vômitos eperda do apetite. Relatam a eliminação abundante deproglotes quando a paciente foi tratada com medicamentos caseiros e a remissão dos sintomas, após dois tratamentos com mebendazol. Contudo não puderam concluir sobre a eficiência das drogas, devido à medicação empírica e ã administração de 200 mg/dia de mebendazo...

  20. RESPONSABILIDADE PELO FATO DO PRODUTO E DO SERVIÇO

    Directory of Open Access Journals (Sweden)

    Michele Romero da Costa*

    2007-12-01

    Full Text Available Este trabalho tem ênfase em apresentar a espécie de responsabilidade adotada pelo Código Brasileiro de Defesa do Consumidor para os fornecedores de produtos e serviços,  qual seja a responsabilidade objetiva, demonstrando uma inovação trazida por este diploma para responsabilidade civil do Direito pátrio. Bem como a sua fundamentação e noções gerais a fim de que se identifiquem as relações em que deve ser o código aplicado, entendidas essas como relações de consumo.

  1. Tratamento da criptococose do sistema nervoso pelo Amphotericin B

    Directory of Open Access Journals (Sweden)

    Dante Giorgi

    1959-12-01

    Full Text Available Os autores descrevem a evolução dos conhecimentos sôbre a meningoen-cefalite produzida pelo Criptococcus neoformans, analisando as dificuldades anteriormente havidas para o diagnóstico em vida, as quais foram superadas depois que se usou rotineiramente a pesquisa do cogumelo no LCR pelo método de coloração de tinta da China e pela cultura. Assinalam, a seguir, o aparecimento do Amphotericin B, novo antibiótico fungicida que veio modificar o prognóstico desta afecção. Apresentam sua experiência com 14 casos de meningite por criptococos diagnosticados em vida e referem pormenorizadamente 2 casos tratados com o Amphotericin B. O primeiro, de paciente portadora desta afecção, evoluindo de maneira crônica, com períodos de exacerbação que melhoram nitidamente com o Amphotericin B e que está com uma sobrevida de 20 meses. O segundo, de paciente portadora de forma aguda desta afecção que teve cura clínica e está com LCR absolutamente normal, com seguimento de 11 meses. Em ambos os casos foi usada a via endovenosa, sendo feitas séries de 10 injeções, cada uma de 50 mg de Amphotericin B em 500 ml de sôro glicosado a 5%. No primeiro caso foram feitas 4 séries e no segundo apenas duas. Não foram registradas reações colaterais de importância. Os autores terminam considerando o Amphotericin B como um elemento que veio tornar muito mais favorável o prognóstico desta afecção até então mortal.

  2. Analytical gradients for tensor hyper-contracted MP2 and SOS-MP2 on graphical processing units

    Science.gov (United States)

    Song, Chenchen; Martínez, Todd J.

    2017-10-01

    Analytic energy gradients for tensor hyper-contraction (THC) are derived and implemented for second-order Møller-Plesset perturbation theory (MP2), with and without the scaled-opposite-spin (SOS)-MP2 approximation. By exploiting the THC factorization, the formal scaling of MP2 and SOS-MP2 gradient calculations with respect to system size is reduced to quartic and cubic, respectively. An efficient implementation has been developed that utilizes both graphics processing units and sparse tensor techniques exploiting spatial sparsity of the atomic orbitals. THC-MP2 has been applied to both geometry optimization and ab initio molecular dynamics (AIMD) simulations. The resulting energy conservation in micro-canonical AIMD demonstrates that the implementation provides accurate nuclear gradients with respect to the THC-MP2 potential energy surfaces.

  3. SOS2 and ACP1 Loci Identified through Large-Scale Exome Chip Analysis Regulate Kidney Development and Function

    DEFF Research Database (Denmark)

    Li, Man; Li, Yong; Weeks, Olivia

    2017-01-01

    Genome-wide association studies have identified >50 common variants associated with kidney function, but these variants do not fully explain the variation in eGFR. We performed a two-stage meta-analysis of associations between genotypes from the Illumina exome array and eGFR on the basis of serum...... creatinine (eGFRcrea) among participants of European ancestry from the CKDGen Consortium (nStage1: 111,666; nStage2: 48,343). In single-variant analyses, we identified single nucleotide polymorphisms at seven new loci associated with eGFRcrea (PPM1J, EDEM3, ACP1, SPEG, EYA4, CYP1A1, and ATXN2L; PStage1......associations of functional rare variants in three genes with eGFRcrea, including a novel association with the SOS Ras/Rho guanine nucleotide exchange factor 2 gene, SOS2 (P=5.4×10(-8) by sequence kernel...

  4. Análise eletromiográfica e força do grupo muscular extensor do punho durante isquemia induzida Electromyographic analysis and strength of the wrist extensor muscle group during induced ischemia

    Directory of Open Access Journals (Sweden)

    CCA Bandeira

    2009-02-01

    Full Text Available OBJETIVO: Avaliar o efeito da isquemia induzida sobre os parâmetros do sinal eletromiográfico e a força do grupo muscular extensor do punho (GMEP em mulheres saudáveis. MÉTODOS: Participaram 13 voluntárias, destras, sedentárias, com idade de 23,38±2,32 anos e índice de massa corporal (IMC de 20,68±1,87kg/m². Para determinar a força do GMEP, foram realizadas 3 contrações isométricas voluntárias máximas (CIVM, utilizando-se uma célula de carga por 15 segundos, com intervalos de 2 minutos entre cada contração, sendo todo procedimento repetido por 3 dias não consecutivos. A isquemia foi realizada por 5 minutos, utilizando um esfigmomanômetro posicionado no braço dominante e inflado até a ausência do fluxo sanguíneo, confirmada pelo ultrassom Doppler. Para coleta do sinal eletromiográfico do GMEP, utilizou-se o equipamento EMG1000 (Lynx® com eletrodo de superfície diferencial (Lynx®. Foram coletadas 3 CIVM por 15 segundos, com intervalo de 30 segundos entre elas, nas situações de pré-isquemia; isquemia; pós-isquemia imediata (pós-1 e pós-isquemia tardia (pós-2 - após 10 minutos do início da isquemia. Para análise dos parâmetros do sinal eletromiográfico, root mean square (RMS, e frequência mediana do espectro de potência do sinal foi utilizado o software MATLAB 6.5.1. Para análise estatística, foram utilizados os testes de Friedman e ANOVA two-way. RESULTADOS: A isquemia promoveu redução significativa (pOBJECTIVE: To analyze the effect of induced ischemia on the parameters of electromyographic signals and the strength of the wrist extensor muscle group (WEMG in healthy women. METHODS: Thirteen right-handed sedentary subjects aged 23.38±2.32 years old, with body mass index (BMI of 20.68±1.87kg/m², took part. To determine WEMG strength, three maximal voluntary isometric contractions (MVIC were performed using a load cell for 15 seconds, with 2 minutes intervals between contractions. The entire

  5. Geographical Simulation and Optimization System (GeoSOS and Its Application in the Analysis of Geographic National Conditions

    Directory of Open Access Journals (Sweden)

    LI Xia

    2017-10-01

    Full Text Available Since the Chinese first survey on geographic national conditions has completed, an urgent need is to analyze these geographical data, such as mining of spatial distribution patterns, land use transition rules, development trends. The analysis is crucial for extracting the knowledge from these big data about geographic national conditions. The remote sensing interpretation data and land use/cover data generated by these geographic national conditions monitoring projects are the basic data sources for a variety of research and applications in terms of land use change detection, urban dynamic analysis, and urban/land use planning. The information can be used for assisting in the coordination of land resource use and decision making for urban and rural development, ecological environment protection and other issues that depends on spatial intelligent decisions. We proposed the theoretical framework of geographical simulation and optimization system (GeoSOS, which coupled geographic process simulation/prediction and spatial optimization, provides powerful theoretical support and practical tools for above researches. This paper develops the extension of GeoSOS software-GeoSOS for ArcGIS, which is an ArcGIS Add-In runs on ArcGIS platform for facilitating the above analyses. We take the urban expansion and ecological protection research in rapid urbanization area as an example, use the software to tackle a series of urbanization issues in the study area. The simulation results show that the predicted land development intensity of Guangdong Province will exceed the constraint index in 2020 according to the national development plan. However, the urbanization expansion based on the constraints of land development intensity and ecological protection can satisfy these constraints, and obtain a more compact landscape pattern. The analysis has shown that GeoSOS can be a useful tool for assisting in the analysis of geographic national conditions information

  6. No evidence of the genotoxic potential of gold, silver, zinc oxide and titanium dioxide nanoparticles in the SOS chromotest.

    Science.gov (United States)

    Nam, Sun-Hwa; Kim, Shin Woong; An, Youn-Joo

    2013-10-01

    Gold nanoparticles (Au NPs), silver nanoparticles (Ag NPs), zinc oxide nanoparticles (ZnO NPs) and titanium dioxide nanoparticles (TiO2 NPs) are widely used in cosmetic products such as preservatives, colorants and sunscreens. This study investigated the genotoxicity of Au NPs, Ag NPs, ZnO NPs and TiO2 NPs using the SOS chromotest with Escherichia coli PQ37. The maximum exposure concentrations for each nanoparticle were 3.23 mg l(-1) for Au NPs, 32.3 mg l(-1) for Ag NPs and 100 mg l(-1) for ZnO NPs and TiO2 NPs. Additionally, in order to compare the genotoxicity of nanoparticles and corresponding dissolved ions, the ions were assessed in the same way as nanoparticles. The genotoxicity of the titanium ion was not assessed because of the extremely low solubility of TiO2 NPs. Au NPs, Ag NPs, ZnO NPs, TiO2 NPs and ions of Au, Ag and Zn, in a range of tested concentrations, exerted no effects in the SOS chromotest, evidenced by maximum IF (IFmax) values of below 1.5 for all chemicals. Owing to the results, nanosized Au NPs, Ag NPs, ZnO NPs, TiO2 NPs and ions of Au, Ag and Zn are classified as non-genotoxic on the basis of the SOS chromotest used in this study. To the best of our knowledge, this is the first study to evaluate the genotoxicity of Au NPs, Ag NPs, ZnO NPs and TiO2 NPs using the SOS chromotest. Copyright © 2012 John Wiley & Sons, Ltd.

  7. Analysis of SOS-Induced Spontaneous Prophage Induction in Corynebacterium glutamicum at the Single-Cell Level

    Science.gov (United States)

    Nanda, Arun M.; Heyer, Antonia; Krämer, Christina; Grünberger, Alexander; Kohlheyer, Dietrich

    2014-01-01

    The genome of the Gram-positive soil bacterium Corynebacterium glutamicum ATCC 13032 contains three integrated prophage elements (CGP1 to -3). Recently, it was shown that the large lysogenic prophage CGP3 (∼187 kbp) is excised spontaneously in a small number of cells. In this study, we provide evidence that a spontaneously induced SOS response is partly responsible for the observed spontaneous CGP3 induction. Whereas previous studies focused mainly on the induction of prophages at the population level, we analyzed the spontaneous CGP3 induction at the single-cell level using promoters of phage genes (Pint2 and Plysin) fused to reporter genes encoding fluorescent proteins. Flow-cytometric analysis revealed a spontaneous CGP3 activity in about 0.01 to 0.08% of the cells grown in standard minimal medium, which displayed a significantly reduced viability. A PrecA-eyfp promoter fusion revealed that a small fraction of C. glutamicum cells (∼0.2%) exhibited a spontaneous induction of the SOS response. Correlation of PrecA to the activity of downstream SOS genes (PdivS and PrecN) confirmed a bona fide induction of this stress response rather than stochastic gene expression. Interestingly, the reporter output of PrecA and CGP3 promoter fusions displayed a positive correlation at the single-cell level (ρ = 0.44 to 0.77). Furthermore, analysis of the PrecA-eyfp/Pint2-e2-crimson strain during growth revealed the highest percentage of spontaneous PrecA and Pint2 activity in the early exponential phase, when fast replication occurs. Based on these studies, we postulate that spontaneously occurring DNA damage induces the SOS response, which in turn triggers the induction of lysogenic prophages. PMID:24163339

  8. Pengaruh Suhu Terhadap Tegangan Permukaan Sabun Cuci Piring Cair Buatan Sendiri, Sunlight, Dan S.O.S

    OpenAIRE

    Siahaan, Okio Patar

    2011-01-01

    The effect of temperature on the surface tension of homemade liquid dish soap, Sunlight, and SOS was carried out. The temperatures was variated 280C(without heating), 300C, 400C and 500C. The homemade liquid dish soap was prepared by using an active ingredients. The active ingredient of the liquid dishwashing soap were sodium lauryl ether sulphate, sodium alkyl benzene sulfonate, and sodium lauril ether sulfate, respectively. The determination of surface tension was based the increasing of t...

  9. Stellar parameters and H α line profile variability of Be stars in the BeSOS survey

    Science.gov (United States)

    Arcos, C.; Kanaan, S.; Chávez, J.; Vanzi, L.; Araya, I.; Curé, M.

    2018-03-01

    The Be phenomenon is present in about 20 per cent of B-type stars. Be stars show variability on a broad range of time-scales, which in most cases is related to the presence of a circumstellar disc of variable size and structure. For this reason, a time-resolved survey is highly desirable in order to understand the mechanisms of disc formation, which are still poorly understood. In addition, a complete observational sample would improve the statistical significance of the study of stellar and disc parameters. The `Be Stars Observation Survey' (BeSOS) is a survey containing reduced spectra obtained using the Pontifica Universidad Católica High Echelle Resolution Optical Spectrograph (PUCHEROS) with a spectral resolution of 17 000 in the range 4260-7300 Å. BeSOS's main objective is to offer consistent spectroscopic and time-resolved data obtained with one instrument. The user can download or plot the data and obtain stellar parameters directly from the website. We also provide a star-by-star analysis based on photometric, spectroscopic and interferometric data, as well as general information about the whole BeSOS sample. Recently, BeSOS led to the discovery of a new Be star HD 42167 and facilitated study of the V/R variation of HD 35165 and HD 120324, the steady disc of HD 110335 and the Be shell status of HD 127972. Optical spectra used in this work, as well as the stellar parameters derived, are available online at http://besos.ifa.uv.cl.

  10. Effect of SOS-induced levels of imuABC on spontaneous and damage-induced mutagenesis in Caulobacter crescentus.

    Science.gov (United States)

    Alves, Ingrid R; Lima-Noronha, Marco A; Silva, Larissa G; Fernández-Silva, Frank S; Freitas, Aline Luiza D; Marques, Marilis V; Galhardo, Rodrigo S

    2017-11-01

    imuABC (imuAB dnaE2) genes are responsible for SOS-mutagenesis in Caulobacter crescentus and other bacterial species devoid of umuDC. In this work, we have constructed operator-constitutive mutants of the imuABC operon. We used this genetic tool to investigate the effect of SOS-induced levels of these genes upon both spontaneous and damage-induced mutagenesis. We showed that constitutive expression of imuABC does not increase spontaneous or damage-induced mutagenesis, nor increases cellular resistance to DNA-damaging agents. Nevertheless, the presence of the operator-constitutive mutation rescues mutagenesis in a recA background, indicating that imuABC are the only genes required at SOS-induced levels for translesion synthesis (TLS) in C. crescentus. Furthermore, these data also show that TLS mediated by ImuABC does not require RecA, unlike umuDC-dependent mutagenesis in E. coli. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. SOS gene induction and possible mutagenic effects of freeze-drying in Escherichia coli and Salmonella typhimurium.

    Science.gov (United States)

    Rosen, Rachel; Buchinger, Sebastian; Pfänder, Ramona; Pedhazur, Rami; Reifferscheid, Georg; Belkin, Shimshon

    2016-11-01

    We report the results of a study of the potential negative effects of the freeze-drying process, normally considered a benign means for long-term conservation of living cells and the golden standard in bacterial preservation. By monitoring gene induction using a whole-cell Escherichia coli bioreporter panel, in which diverse stress-responsive gene promoters are fused to luminescent or fluorescent reporting systems, we have demonstrated that DNA repair genes belonging to the SOS operon (recA, sulA, uvrA, umuD, and lexA) were induced upon resuscitation from the freeze-dried state, whereas other stress-responsive promoters such as grpE, katG, phoA, soxS, and sodA were not affected. This observation was confirmed by the UMU-chromotest (activation of the umuD gene promoter) in Salmonella typhimurium, as well as by real-time PCR analyses of selected E. coli SOS genes. We further show that a functional SOS operon is important in viability maintenance following resuscitation, but that at the same time, this repair system may introduce significantly higher mutation rates, comparable to those induced by high concentrations of a known mutagen. Our results also indicate that the entire freeze-drying process, rather than either freezing or drying separately, is instrumental in the induction of DNA damage.

  12. Competitive fitness during feast and famine: how SOS DNA polymerases influence physiology and evolution in Escherichia coli.

    Science.gov (United States)

    Corzett, Christopher H; Goodman, Myron F; Finkel, Steven E

    2013-06-01

    Escherichia coli DNA polymerases (Pol) II, IV, and V serve dual roles by facilitating efficient translesion DNA synthesis while simultaneously introducing genetic variation that can promote adaptive evolution. Here we show that these alternative polymerases are induced as cells transition from exponential to long-term stationary-phase growth in the absence of induction of the SOS regulon by external agents that damage DNA. By monitoring the relative fitness of isogenic mutant strains expressing only one alternative polymerase over time, spanning hours to weeks, we establish distinct growth phase-dependent hierarchies of polymerase mutant strain competitiveness. Pol II confers a significant physiological advantage by facilitating efficient replication and creating genetic diversity during periods of rapid growth. Pol IV and Pol V make the largest contributions to evolutionary fitness during long-term stationary phase. Consistent with their roles providing both a physiological and an adaptive advantage during stationary phase, the expression patterns of all three SOS polymerases change during the transition from log phase to long-term stationary phase. Compared to the alternative polymerases, Pol III transcription dominates during mid-exponential phase; however, its abundance decreases to SOS induction by exogenous agents and indicate that cell populations require appropriate expression of all three alternative DNA polymerases during exponential, stationary, and long-term stationary phases to attain optimal fitness and undergo adaptive evolution.

  13. The SbSOS1 gene from the extreme halophyte Salicornia brachiata enhances Na+ loading in xylem and confers salt tolerance in transgenic tobacco

    Directory of Open Access Journals (Sweden)

    Yadav Narendra

    2012-10-01

    Full Text Available Abstract Background Soil salinity adversely affects plant growth and development and disturbs intracellular ion homeostasis resulting cellular toxicity. The Salt Overly Sensitive 1 (SOS1 gene encodes a plasma membrane Na+/H+ antiporter that plays an important role in imparting salt stress tolerance to plants. Here, we report the cloning and characterisation of the SbSOS1 gene from Salicornia brachiata, an extreme halophyte. Results The SbSOS1 gene is 3774 bp long and encodes a protein of 1159 amino acids. SbSOS1 exhibited a greater level of constitutive expression in roots than in shoots and was further increased by salt stress. Overexpressing the S. brachiata SbSOS1 gene in tobacco conferred high salt tolerance, promoted seed germination and increased root length, shoot length, leaf area, fresh weight, dry weight, relative water content (RWC, chlorophyll, K+/Na+ ratio, membrane stability index, soluble sugar, proline and amino acid content relative to wild type (WT plants. Transgenic plants exhibited reductions in electrolyte leakage, reactive oxygen species (ROS and MDA content in response to salt stress, which probably occurred because of reduced cytosolic Na+ content and oxidative damage. At higher salt stress, transgenic tobacco plants exhibited reduced Na+ content in root and leaf and higher concentrations in stem and xylem sap relative to WT, which suggests a role of SbSOS1 in Na+ loading to xylem from root and leaf tissues. Transgenic lines also showed increased K+ and Ca2+ content in root tissue compared to WT, which reflect that SbSOS1 indirectly affects the other transporters activity. Conclusions Overexpression of SbSOS1 in tobacco conferred a high degree of salt tolerance, enhanced plant growth and altered physiological and biochemical parameters in response to salt stress. In addition to Na+ efflux outside the plasma membrane, SbSOS1 also helps to maintain variable Na+ content in different organs and also affect the other

  14. The SbSOS1 gene from the extreme halophyte Salicornia brachiata enhances Na(+) loading in xylem and confers salt tolerance in transgenic tobacco.

    Science.gov (United States)

    Yadav, Narendra Singh; Shukla, Pushp Sheel; Jha, Anupama; Agarwal, Pradeep K; Jha, Bhavanath

    2012-10-11

    Soil salinity adversely affects plant growth and development and disturbs intracellular ion homeostasis resulting cellular toxicity. The Salt Overly Sensitive 1 (SOS1) gene encodes a plasma membrane Na(+)/H(+) antiporter that plays an important role in imparting salt stress tolerance to plants. Here, we report the cloning and characterisation of the SbSOS1 gene from Salicornia brachiata, an extreme halophyte. The SbSOS1 gene is 3774 bp long and encodes a protein of 1159 amino acids. SbSOS1 exhibited a greater level of constitutive expression in roots than in shoots and was further increased by salt stress. Overexpressing the S. brachiata SbSOS1 gene in tobacco conferred high salt tolerance, promoted seed germination and increased root length, shoot length, leaf area, fresh weight, dry weight, relative water content (RWC), chlorophyll, K(+)/Na(+) ratio, membrane stability index, soluble sugar, proline and amino acid content relative to wild type (WT) plants. Transgenic plants exhibited reductions in electrolyte leakage, reactive oxygen species (ROS) and MDA content in response to salt stress, which probably occurred because of reduced cytosolic Na(+) content and oxidative damage. At higher salt stress, transgenic tobacco plants exhibited reduced Na(+) content in root and leaf and higher concentrations in stem and xylem sap relative to WT, which suggests a role of SbSOS1 in Na(+) loading to xylem from root and leaf tissues. Transgenic lines also showed increased K(+) and Ca(2+) content in root tissue compared to WT, which reflect that SbSOS1 indirectly affects the other transporters activity. Overexpression of SbSOS1 in tobacco conferred a high degree of salt tolerance, enhanced plant growth and altered physiological and biochemical parameters in response to salt stress. In addition to Na(+) efflux outside the plasma membrane, SbSOS1 also helps to maintain variable Na(+) content in different organs and also affect the other transporters activity indirectly. These

  15. The SbSOS1 gene from the extreme halophyte Salicornia brachiata enhances Na+ loading in xylem and confers salt tolerance in transgenic tobacco

    Science.gov (United States)

    2012-01-01

    Background Soil salinity adversely affects plant growth and development and disturbs intracellular ion homeostasis resulting cellular toxicity. The Salt Overly Sensitive 1 (SOS1) gene encodes a plasma membrane Na+/H+ antiporter that plays an important role in imparting salt stress tolerance to plants. Here, we report the cloning and characterisation of the SbSOS1 gene from Salicornia brachiata, an extreme halophyte. Results The SbSOS1 gene is 3774 bp long and encodes a protein of 1159 amino acids. SbSOS1 exhibited a greater level of constitutive expression in roots than in shoots and was further increased by salt stress. Overexpressing the S. brachiata SbSOS1 gene in tobacco conferred high salt tolerance, promoted seed germination and increased root length, shoot length, leaf area, fresh weight, dry weight, relative water content (RWC), chlorophyll, K+/Na+ ratio, membrane stability index, soluble sugar, proline and amino acid content relative to wild type (WT) plants. Transgenic plants exhibited reductions in electrolyte leakage, reactive oxygen species (ROS) and MDA content in response to salt stress, which probably occurred because of reduced cytosolic Na+ content and oxidative damage. At higher salt stress, transgenic tobacco plants exhibited reduced Na+ content in root and leaf and higher concentrations in stem and xylem sap relative to WT, which suggests a role of SbSOS1 in Na+ loading to xylem from root and leaf tissues. Transgenic lines also showed increased K+ and Ca2+ content in root tissue compared to WT, which reflect that SbSOS1 indirectly affects the other transporters activity. Conclusions Overexpression of SbSOS1 in tobacco conferred a high degree of salt tolerance, enhanced plant growth and altered physiological and biochemical parameters in response to salt stress. In addition to Na+ efflux outside the plasma membrane, SbSOS1 also helps to maintain variable Na+ content in different organs and also affect the other transporters activity indirectly

  16. Resposta imune induzida por antígenos de Mycoplasma hyopneumoniae avaliados como vacina de DNA ou subunidade recombinante.

    OpenAIRE

    Galli, Vanessa

    2011-01-01

    Mycoplasma hyopneumoniae é o agente etiológico da Pneumonia Enzoótica Suína (PES), uma das doenças respiratórias de maior incidência na criação de suínos no mundo. As vacinas disponíveis comercialmente consistem de células inteiras inativadas (bacterina), as quais proporcionam apenas uma proteção parcial e não previnem a colonização pelo microrganismo. Neste contexto, faz-se necessária a busca de novas alternativas para a profilaxia da PES. Alguns antígenos vêm sendo testado...

  17. Adherence to abiotic surface induces SOS response in Escherichia coli K-12 strains under aerobic and anaerobic conditions.

    Science.gov (United States)

    Costa, Suelen B; Campos, Ana Carolina C; Pereira, Ana Claudia M; de Mattos-Guaraldi, Ana Luiza; Júnior, Raphael Hirata; Rosa, Ana Cláudia P; Asad, Lídia M B O

    2014-09-01

    During the colonization of surfaces, Escherichia coli bacteria often encounter DNA-damaging agents and these agents can induce several defence mechanisms. Base excision repair (BER) is dedicated to the repair of oxidative DNA damage caused by reactive oxygen species (ROS) generated by chemical and physical agents or by metabolism. In this work, we have evaluated whether the interaction with an abiotic surface by mutants derived from E. coli K-12 deficient in some enzymes that are part of BER causes DNA damage and associated filamentation. Moreover, we studied the role of endonuclease V (nfi gene; 1506 mutant strain) in biofilm formation. Endonuclease V is an enzyme that is involved in DNA repair of nitrosative lesions. We verified that endonuclease V is involved in biofilm formation. Our results showed more filamentation in the xthA mutant (BW9091) and triple xthA nfo nth mutant (BW535) than in the wild-type strain (AB1157). By contrast, the mutant nfi did not present filamentation in biofilm, although its wild-type strain (1466) showed rare filaments in biofilm. The filamentation of bacterial cells attaching to a surface was a consequence of SOS induction measured by the SOS chromotest. However, biofilm formation depended on the ability of the bacteria to induce the SOS response since the mutant lexA Ind(-) did not induce the SOS response and did not form any biofilm. Oxygen tension was an important factor for the interaction of the BER mutants, since these mutants exhibited decreased quantitative adherence under anaerobic conditions. However, our results showed that the presence or absence of oxygen did not affect the viability of BW9091 and BW535 strains. The nfi mutant and its wild-type did not exhibit decreased biofilm formation under anaerobic conditions. Scanning electron microscopy was also performed on the E. coli K-12 strains that had adhered to the glass, and we observed the presence of a structure similar to an extracellular matrix that depended on the

  18. Sejong Open Cluster Survey (SOS). 0. Target Selection and Data Analysis

    Science.gov (United States)

    Sung, Hwankyung; Lim, Beomdu; Bessell, Michael S.; Kim, Jinyoung S.; Hur, Hyeonoh; Chun, Moo-Young; Park, Byeong-Gon

    2013-06-01

    Star clusters are superb astrophysical laboratories containing cospatial and coeval samples of stars with similar chemical composition. We initiate the Sejong Open cluster Survey (SOS) - a project dedicated to providing homogeneous photometry of a large number of open clusters in the SAAO Johnson-Cousins' UBVI system. To achieve our main goal, we pay much attention to the observation of standard stars in order to reproduce the SAAO standard system. Many of our targets are relatively small sparse clusters that escaped previous observations. As clusters are considered building blocks of the Galactic disk, their physical properties such as the initial mass function, the pattern of mass segregation, etc. give valuable information on the formation and evolution of the Galactic disk. The spatial distribution of young open clusters will be used to revise the local spiral arm structure of the Galaxy. In addition, the homogeneous data can also be used to test stellar evolutionary theory, especially concerning rare massive stars. In this paper we present the target selection criteria, the observational strategy for accurate photometry, and the adopted calibrations for data analysis such as color-color relations, zero-age main sequence relations, Sp - M_V relations, Sp - T_{eff} relations, Sp - color relations, and T_{eff} - BC relations. Finally we provide some data analysis such as the determination of the reddening law, the membership selection criteria, and distance determination.

  19. Comparison of VOC measurements in Nashville, TN, during the southern oxidants study (SOS) 1999

    International Nuclear Information System (INIS)

    Grabmer, W.; Wisthaler, A.; Hansel, A.; Stroud, C.; Roberts, J.M.; Fehsenfeld, F.C.

    2002-01-01

    Full text: During the Southern Oxidants Study (SOS) 1999 Nashville campaign ambient air samples were analyzed at Cornelia Fort Airport (CFA) for organic compounds by two independent methods: 1) a gas chromatographic systems operated by NOAAs Aeronomy Laboratory, which performed immediate analysis of collected samples and 2) an in situ proton transfer reaction mass spectrometer (PTR-MS) system operated by the Univ. of Innsbruck. The sample protocols were quite different for the different methods. The GC system sequentially collected and analyzed air samples each 60 minutes for VOCs. The in-situ PTR-MS system measured more than 20 VOCs on a time shared basis for 5 to 15 seconds respectively, once each 5 minutes. The PTR-MS system is not able to distinguish between isobaric species, therefore acetone and propanal (MVK and MACR) values measured by NOAAs GC were added up prior to comparison with the respective PTR-MS values. For all species mentioned above the different measurement methods show good agreement. (author)

  20. Repair promoted by plasmid pKM101 is different from SOS repair

    International Nuclear Information System (INIS)

    Goze, A.; Devoret, R.

    1979-01-01

    In E. coli K12 bacteria carrying plasmid pKM101, prophage lambda was induced at UV doses higher than in plasmid-less parental bacteria. UV-induced reactivation per se was less effective. Bacteria with pKM101 showed no alteration in their division cycle. Plasmid PKM101 coded for a constitutive error-prone repair different from the inducible error-prone repair called SOS repair. Plasmid pKM101 protected E. coli bacteria from UV damage but slightly sensitized them to X-ray lesions. Protection against UV damage was effective in mutant bacteria deficient in DNA excision-repair provided that the recA, lexA and uvrE genes were functional. Survival of phages lambda and S13 after UV irradiation was enhanced in bacteria carrying plasmid pKM101; phage lambda mutagenesis was also increased. Plasmid pKM101 repaired potentially lethal DNA lesions, although Wild-type DNA sequences may not necessarily be restored; hence the mutations observed are the traces of the original DNA lesions. (Auth.)

  1. Impaired recovery and mutagenic SOS-like responses in ataxia telangiectasia cells

    Energy Technology Data Exchange (ETDEWEB)

    Hilgers, G. (Universite Libre de Bruxelles (Belgium) Rijksuniversiteit Leiden (Netherlands)); Abrahams, P.J. (Rijksuniversiteit Leiden (Netherlands)); Chen, Y.Q. (Universite Libre de Bruxelles (Belgium)); Schouten, R. (Rijksuniversiteit Leiden (Netherlands)); Cornelis, J.J. (Universite Libre de Bruxelles (Belgium) Institut Pasteur, 75 - Paris (France)); Lowe, J.E. (Sussex Univ., Brighton (UK)); Eb, A.J. van der (Rijksuniversiteit Leiden (Netherlands)); Rommelaere, J. (Universite Libre de Bruxelles (Belgium) Institut Pasteur, 75 - Paris (France))

    1989-01-01

    Radiosensitive fibroblasts from patients with ataxia telangiectasia (AT) were studied for their proficiency in two putative eukaryotic SOS-like responses, namely the enhanced reactivation (ER) and enhanced mutagenesis of damaged viruses infecting pre-irradiated versus mock-treated cells. A previous report indicated that, unlike normal human cells, a line of AT fibroblasts (AT5BIVA) could not be induced to express ER of damaged parvovirus H-1, a single-stranded DNA virus, by UV- or X-irradiation. In the present study, AT5BIVA fibroblasts were also distinguished from normal cells by the inability of the former to achieve enhanced mutagenesis of damaged H-1 virus upon cell UV-irradiation. In contrast, dose-response and time-course experiments revealed normal levels of ER of Herpes simplex virus 1, a double-stranded DNA virus, in X- or UV-irradiated AT5BIVA cells. Taken together, these data point to a possible deficiency of AT cells in a conditioned mutagenic process that contributes to a greater extent to the recovery of damaged single-stranded than double-stranded DNA. Such a defect may concern the replication of damaged DNA or the generation of signals promoting the latter process and may be related to the lack of radiation-induced delay that is typical of AT cell DNA synthesis. (author).

  2. Impaired recovery and mutagenic SOS-like responses in ataxia telangiectasia cells

    International Nuclear Information System (INIS)

    Hilgers, G.; Abrahams, P.J.; Chen, Y.Q.; Schouten, R.; Cornelis, J.J.; Lowe, J.E.; Eb, A.J. van der; Rommelaere, J.

    1989-01-01

    Radiosensitive fibroblasts from patients with ataxia telangiectasia (AT) were studied for their proficiency in two putative eukaryotic SOS-like responses, namely the enhanced reactivation (ER) and enhanced mutagenesis of damaged viruses infecting pre-irradiated versus mock-treated cells. A previous report indicated that, unlike normal human cells, a line of AT fibroblasts (AT5BIVA) could not be induced to express ER of damaged parvovirus H-1, a single-stranded DNA virus, by UV- or X-irradiation. In the present study, AT5BIVA fibroblasts were also distinguished from normal cells by the inability of the former to achieve enhanced mutagenesis of damaged H-1 virus upon cell UV-irradiation. In contrast, dose-response and time-course experiments revealed normal levels of ER of Herpes simplex virus 1, a double-stranded DNA virus, in X- or UV-irradiated AT5BIVA cells. Taken together, these data point to a possible deficiency of AT cells in a conditioned mutagenic process that contributes to a greater extent to the recovery of damaged single-stranded than double-stranded DNA. Such a defect may concern the replication of damaged DNA or the generation of signals promoting the latter process and may be related to the lack of radiation-induced delay that is typical of AT cell DNA synthesis. (author)

  3. Comparison of VOC measurements in Nashville, TE, during the southern oxidants study (SOS) 1999

    International Nuclear Information System (INIS)

    Grabmer, W.; Wisthaler, A.; Hansel, A.; Stroud, C.; Roberts, J.M.; Fehsenfeld, F.C.

    2002-01-01

    During the Southern Oxidants Study (SOS) 1999 Nashville campaign ambient air samples were analyzed at Cornelia Fort Airport (CFA) for organic compounds by two independent methods: 1) a gas chromatographic systems operated by NOAAs Aeronomy Laboratory, which performed immediate analysis of collected samples and 2) an in situ proton transfer reaction mass spectrometer (PTR M S) system operated by the University of Innsbruck. The sample protocols were quite different for the different methods. The GC system sequentially collected and analyzed air samples each 60 minutes for VOCs. The in-situ PTR-MS system measured more than 20 VOCs on a time shared basis for 5 to 15 seconds respectively, once each five minutes. The PTR-MS system is not able to distinguish between isobaric species, therefor acetone and propanal (MVK and MACR) values measured by NOAAs GC were added up prior to comparison with the respective PTR-MS values. For all species mentioned above the different measurement methods show good agreement. (author)

  4. The SOS chromotest: bacterial cells to detect and characterize genotoxic products and radiations

    International Nuclear Information System (INIS)

    Quillardet, P.; Hofnung, M.

    1994-01-01

    The advanced knowledge we have on the bacterium Escherichia coli has facilitated the development of the colorimetric and fast assay, the SOS chromotest, which involves a single tester strain and gives a qualitative and a quantitative assay of the action of a genotoxic agent. We discuss a number of possibilities opened by this test in order to make a genetic diagnosis of the chemical nature of the damages caused in the genetic material by means of a battery of strains which have been genetically modified for that purpose. In order to give an idea of the accuracy of the bacterial responses and of the way they can be used to characterize the DNA damage by a genetic approach, the case of alkylating agents is described in a relatively detailed fashion, and the case of oxidative agents is rapidly mentioned. The sensitivity to ionizing radiation is such that the test is able to detect doses of the order of 1 Gy. We discuss briefly how it could be possible to increase this sensitivity by genetically inactivating repair systems which process the injuries caused by these agents, and how the use of a battery of tester strains could also give information on the nature of injuries caused by various types of ionizing radiation. (authors). 39 refs. 4 figs. 1 tab

  5. Lung cancer detection with digital chest tomosynthesis: first round results from the SOS observational study.

    Science.gov (United States)

    Bertolaccini, Luca; Viti, Andrea; Tavella, Chiara; Priotto, Roberto; Ghirardo, Donatella; Grosso, Maurizio; Terzi, Alberto

    2015-04-01

    Baseline results of the Studio OSservazionale (SOS), observational study, a single-arm observational study of digital chest tomosynthesis for lung cancer detection in an at-risk population demonstrated a detection rate of lung cancer comparable to that of studies that used low dose CT scan (LDCT). We present the results of the first round. Totally 1,703 out of 1,843 (92%) subjects who had a baseline digital chest tomosynthesis underwent a first round reevaluation after 1 year. At first round chest digital tomosynthesis, 13 (0.7%) subjects had an indeterminate nodule larger than 5 mm and underwent low-dose CT scan for nodule confirmation. PET/CT study was obtained in 10 (0.5%) subjects and 2 subjects had a low-dose CT follow up. Surgery, either video-assisted thoracoscopic or open surgery for indeterminate pulmonary nodules was performed in 10 (0.2%) subjects. A lung cancer was diagnosed and resected in five patients. The lung cancer detection rate at first round was 0.3% (5/1,703). The detection rate of lung cancer at first round for tomosynthesis is comparable to rates reported for CT. In addition, results of first round digital chest tomosynthesis confirm chest tomosynthesis as a possible first-line lung cancer-screening tool.

  6. Inhibition of the SOS response of Escherichia coli by the Ada protein

    International Nuclear Information System (INIS)

    Vericat, J.A.; Guerrero, R.; Barbe, J.

    1988-01-01

    Induction of the adaptive response by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) caused a decrease in the UV-mediated expression of both recA and sfiA genes but not of the umuDC gene. On the other hand, the adaptive response did not affect the temperature-promoted induction of SOS response in a RecA441 mutant. The inhibitory effect on the UV-triggered expression of the recA and sfiA genes was not dependent on either the alkA gene or the basal level of RecA protein, but rather required the ada gene. Furthermore, an increase in the level of the Ada protein, caused by the runaway plasmid pYN3059 in which the ada gene is regulated by the lac promoter, inhibited UV-mediated recA gene expression even in cells to which the MNNG-adaptive treatment had not been applied. This inhibitory effect of the adaptive pretreatment was not observed either in RecBC- strains or in RecBC mutants lacking exonuclease V-related nuclease activity. However, RecF- mutants showed an adaptive response-mediated decrease in UV-promoted induction of the recA gene

  7. Genetic separation of Escherichia coli recA functions for SOS mutagenesis and repressor cleavage

    International Nuclear Information System (INIS)

    Ennis, D.G.; Ossanna, N.; Mount, D.W.

    1989-01-01

    Evidence is presented that recA functions which promote the SOS functions of mutagenesis, LexA protein proteolysis, and lambda cI repressor proteolysis are each genetically separable from the others. This separation was observed in recombination-proficient recA mutants and rec+ (F' recA56) heterodiploids. recA430, recA433, and recA435 mutants and recA+ (F' recA56) heterodiploids were inducible for only one or two of the three functions and defective for mutagenesis. recA80 and recA432 mutants were constitutively activated for two of the three functions in that these mutants did not have to be induced to express the functions. We propose that binding of RecA protein to damaged DNA and subsequent interaction with small inducer molecules gives rise to conformational changes in RecA protein. These changes promote surface-surface interactions with other target proteins, such as cI and LexA proteins. By this model, the recA mutants are likely to have incorrect amino acids substituted as sites in the RecA protein structure which affect surface regions required for protein-protein interactions. The constitutively activated mutants could likewise insert altered amino acids at sites in RecA which are involved in the activation of RecA protein by binding small molecules or polynucleotides which metabolically regulate RecA protein

  8. Activation of Extracellular Signal-Regulated Kinase but Not of p38 Mitogen-Activated Protein Kinase Pathways in Lymphocytes Requires Allosteric Activation of SOS

    Science.gov (United States)

    Jun, Jesse E.; Yang, Ming; Chen, Hang; Chakraborty, Arup K.

    2013-01-01

    Thymocytes convert graded T cell receptor (TCR) signals into positive selection or deletion, and activation of extracellular signal-related kinase (ERK), p38, and Jun N-terminal protein kinase (JNK) mitogen-activated protein kinases (MAPKs) has been postulated to play a discriminatory role. Two families of Ras guanine nucleotide exchange factors (RasGEFs), SOS and RasGRP, activate Ras and the downstream RAF-MEK-ERK pathway. The pathways leading to lymphocyte p38 and JNK activation are less well defined. We previously described how RasGRP alone induces analog Ras-ERK activation while SOS and RasGRP cooperate to establish bimodal ERK activation. Here we employed computational modeling and biochemical experiments with model cell lines and thymocytes to show that TCR-induced ERK activation grows exponentially in thymocytes and that a W729E allosteric pocket mutant, SOS1, can only reconstitute analog ERK signaling. In agreement with RasGRP allosterically priming SOS, exponential ERK activation is severely decreased by pharmacological or genetic perturbation of the phospholipase Cγ (PLCγ)-diacylglycerol-RasGRP1 pathway. In contrast, p38 activation is not sharply thresholded and requires high-level TCR signal input. Rac and p38 activation depends on SOS1 expression but not allosteric activation. Based on computational predictions and experiments exploring whether SOS functions as a RacGEF or adaptor in Rac-p38 activation, we established that the presence of SOS1, but not its enzymatic activity, is critical for p38 activation. PMID:23589333

  9. Dismorfia muscular: A busca pelo corpo hiper musculoso

    Directory of Open Access Journals (Sweden)

    Andréa Pires Azevedo

    2012-03-01

    Full Text Available A dismorfia muscular tem sido identificada tanto em homens quanto em mulheres, provocando alterações da perceção da autoimagem e prejuízos socioculturais, e na saúde e bem-estar dos indivíduos. Nesse sentido, o objetivo deste estudo é analisar os aspetos socioculturais, psicológicos e o uso de recursos ergogênicos relacionados à dismorfia muscular, bem como, identificar os riscos promovidos pelo transtorno. Participaram 20 indivíduos, inscritos na rede mundial de computadores. Utilizou-se um questionário para coleta de dados e a análise documental para interpretação das respostas. Os resultados demonstram que as preocupações com a imagem corporal geram insegurança social, baixa autoestima e sentimentos de inferioridade, que seriam resolvidos se a pessoa tivesse corpos belos e fortes. A dismorfia muscular pode aumentar o risco de uso dos esteroides anabolizantes e o uso indiscriminado de suplementos alimentares. Conclui-se que a dismorfia muscular causa sofrimentos e prejuízos psicológicos, socioculturais e, desse modo, compromete a saúde das pessoas.

  10. O conhecimento pré-colombiano do Brasil pelos portugueses

    Directory of Open Access Journals (Sweden)

    Thomaz Oscar Marcondes de Souza

    1961-09-01

    Full Text Available A brilhante emprêsa marítima realizada pelo infante D. Henrique ao longo da costa ocidental da África; a passagem do cabo da Boa Esperança em 1488 por Bartolomeu Dias; o desco-brimento do caminho marítimo para a índia por Vasco da Ga-ma em 1498; as guerras e conquistas na Ásia com a figura ím-par de Afonso de Albuquerque; o ter Portugal mantido por mais de três séculos a integridade territorial do Brasil, vencendo fran-ceses, holandeses e espanhóis, não satisfazem a vaidade nacio-nal de certos historiadores portuguêses . Querem mais . Que-rem que tudo que possa constituir prioridade, em se tratando de descobrimento marítimos, pertença a Portugal. Navegado-res do quilate de Colombo, Caboto, Vespucci, Pinzon, Verraz-zano, Cartier e outros, não passam de medíocres, de embustei-ros e de falsos descobridores que se apropriaram de glórias alheias, sendo que o Genovês, o pouco que sabia de navegação, aprendeu em Portugal.

  11. The SH2 and SH3 domains of mammalian Grb2 couple the EGF receptor to the Ras activator mSos1.

    Science.gov (United States)

    Rozakis-Adcock, M; Fernley, R; Wade, J; Pawson, T; Bowtell, D

    1993-05-06

    Many tyrosine kinases, including the receptors for hormones such as epidermal growth factor (EGF), nerve growth factor and insulin, transmit intracellular signals through Ras proteins. Ligand binding to such receptors stimulates Ras guanine-nucleotide-exchange activity and increases the level of GTP-bound Ras, suggesting that these tyrosine kinases may activate a guanine-nucleotide releasing protein (GNRP). In Caenorhabditis elegans and Drosophila, genetic studies have shown that Ras activation by tyrosine kinases requires the protein Sem-5/drk, which contains a single Src-homology (SH) 2 domain and two flanking SH3 domains. Sem-5 is homologous to the mammalian protein Grb2, which binds the autophosphorylated EGF receptor and other phosphotyrosine-containing proteins such as Shc through its SH2 domain. Here we show that in rodent fibroblasts, the SH3 domains of Grb2 are bound to the proline-rich carboxy-terminal tail of mSos1, a protein homologous to Drosophila Sos. Sos is required for Ras signalling and contains a central domain related to known Ras-GNRPs. EGF stimulation induces binding of the Grb2-mSos1 complex to the autophosphorylated EGF receptor, and mSos1 phosphorylation. Grb2 therefore appears to link tyrosine kinases to a Ras-GNRP in mammalian cells.

  12. Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity.

    Science.gov (United States)

    Moreb, Eirik Adim; Hoover, Benjamin; Yaseen, Adam; Valyasevi, Nisakorn; Roecker, Zoe; Menacho-Melgar, Romel; Lynch, Michael D

    2017-12-15

    Phage-derived "recombineering" methods are utilized for bacterial genome editing. Recombineering results in a heterogeneous population of modified and unmodified chromosomes, and therefore selection methods, such as CRISPR-Cas9, are required to select for edited clones. Cells can evade CRISPR-Cas-induced cell death through recA-mediated induction of the SOS response. The SOS response increases RecA dependent repair as well as mutation rates through induction of the umuDC error prone polymerase. As a result, CRISPR-Cas selection is more efficient in recA mutants. We report an approach to inhibiting the SOS response and RecA activity through the expression of a mutant dominant negative form of RecA, which incorporates into wild type RecA filaments and inhibits activity. Using a plasmid-based system in which Cas9 and recA mutants are coexpressed, we can achieve increased efficiency and consistency of CRISPR-Cas9-mediated selection and recombineering in E. coli, while reducing the induction of the SOS response. To date, this approach has been shown to be independent of recA genotype and host strain lineage. Using this system, we demonstrate increased CRISPR-Cas selection efficacy with over 10 000 guides covering the E. coli chromosome. The use of dominant negative RecA or homologues may be of broad use in bacterial CRISPR-Cas-based genome editing where the SOS pathways are present.

  13. Hepatite aguda colestática pelo propiltiouracil: relato de caso

    Directory of Open Access Journals (Sweden)

    PAROLIN Mônica Beatriz

    2000-01-01

    Full Text Available Propiltiouracil é uma droga amplamente utilizada no tratamento do hipertiroidismo. A hepatotoxicidade é um dos efeitos colaterais mais raros e também mais graves associados a ela. Relata-se um caso de hepatite aguda colestática que acomete um jovem de 15 anos em uso de propiltiouracil para tratamento de hipertiroidismo. Causas virais, metabólicas e autoimunes foram excluídas e a biopsia hepática revelou achados histopatológicos sugestivos de hepatite colestática induzida por droga. Com a suspensão da droga, houve remissão dos sintomas e normalização progressiva das provas de função hepática. Raramente, os pacientes em uso de propiltiouracil podem desenvolver injúria hepática grave.

  14. Effects of ligature-induced periodontitis in pregnant Wistar rats Efeito da doença periodontal induzida por ligadura na prenhez de ratas Wistar

    Directory of Open Access Journals (Sweden)

    Mariane Ponzio de Azevedo Galvão

    2003-03-01

    Full Text Available The aim of this study was to evaluate the influence of ligature-induced periodontal disease in pregnant rats on their newborn's health parameters. Twenty-four female adult Wistar rats were divided into two groups: the control group (G1 and the group that was submitted to dental ligatures around second upper molars (G2. After the four week period of development of periodontitis, the female animals were mated with male adult Wistar rats. There were no differences in the body weight of females between the two groups during mating and pregnancy. No differences were observed among the groups in relation to the viable newborn index. However, there were differences in newborn birth weight, explained by the diverse size of the litters. In this study, ligature-induced periodontal disease did not promote changes during pregnancy that resulted in low birth weight in newborn Wistar rats.O objetivo do presente estudo foi avaliar a influência da periodontite induzida por ligadura em ratas prenhes sobre parâmetros de saúde geral de seus filhotes. Vinte e quatro ratas Wistar de idade adulta foram divididas em dois grupos: grupo controle (G1 e grupo experimental, que recebeu ligaduras ao redor dos segundos molares superiores (G2. Após o período de indução de periodontite (quatro semanas, as ratas foram colocadas para cruzamento com ratos Wistar machos, adultos. Não houve diferença no peso corporal das fêmeas durante os períodos de cruzamento e prenhez. Também não foram observadas diferenças entre os grupos quanto à taxa de recém-nascidos viáveis. No entanto, houve diferença quanto ao peso dos recém-nascidos, sendo tal diferença explicada pela variação no tamanho das ninhadas. No presente estudo, doença periodontal induzida por ligadura não promoveu mudanças durante a prenhez que resultassem em baixo peso ao nascer dos filhotes Wistar.

  15. Infestaçao humana pelo Dipylidium caninum

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    Carlos Henrique Lemos

    1985-12-01

    Full Text Available Os autores descrevem um caso de infestação pelo Dipylidium caninum numa criança residente em Araguari, Minas Gerais, que apresentou lesões cutâneas, insônia, irritabilidade, vômitos eperda do apetite. Relatam a eliminação abundante deproglotes quando a paciente foi tratada com medicamentos caseiros e a remissão dos sintomas, após dois tratamentos com mebendazol. Contudo não puderam concluir sobre a eficiência das drogas, devido à medicação empírica e ã administração de 200 mg/dia de mebendazol, contrariando a dosagem recomendada para Cestodas, que é de 400 mg/dia. Relatam, ainda, que exames das fezes do cão de propriedade da família revelaram apenas a presença de ancilostomídeos.The authors describe a case of Dipylidium caninum infestation in a child living in Araguari, state of Minas Gerais, Brazil, who has presented skin lesions, insomnia, irritability, vomitingand lossof appetite. When the patient was treated with folk medicine they noticed excessive elimination of proglotids and the elimination of symptoms after being treated twice with mebendazol. However it was not possible to conclude anything about the drugs, due to the empiric medication and the administration of mebendazol in a dose of200 mg daily, which is not the quantity recommended for Cestodes (400 mg daily. The fecal examination of the dog that belongs to the family showed only ancylostomides.

  16. Experimental and simulation study of the effects of cosmic particles on CMOS/SOS RAMs

    International Nuclear Information System (INIS)

    Worley, E.; Williams, R.; Groninger, J.

    1990-01-01

    Van De Graaff particle accelerator data was taken on three different CMOS/SOS RAM cells. The resulting LET upset thresholds were then used to calculate the deposited charge needed to upset the cells. Detailed SPICE simulations of the various cells were used to determine the collected charge required for upset. A comparison of the two values indicated that the charge needed to upset the cells was greater than the deposited charge, thus confirming Rollins' results. Shorter channel lengths and higher power supply voltages caused the ratio, M, of upset charge to deposited charge to increase. As a result of this multiplication factor, actual devices are more likely to upset (i.e., upset at lower energy) than expected from an analysis of only the collected charge. A mixed mode simulator was then used to model the charge collection process. This simulator study showed that the M factor is a very fluid number which is dependent on minority carrier lifetime, drain voltage, and the switching dynamics of the cell in addition to the dependence on mobility ratio and channel length. Parasitic bipolar gain at high injection levels appears to be the primary mechanism allowing collected charge to be greater than deposited charge. In conclusion, the simulator and experimental data show that, as floating body static memory transistors are down scaled, the particle energy needed to upset the cell is reduced because of the enhanced parasitic bipolar gain effect as well as a reduction in the node capacitance. This result is shown by simulation to also apply to fully depleted SOI transistors

  17. A System of Systems (SoS) Approach to Sustainable Energy Planning in MENA

    Science.gov (United States)

    Mahlooji, Maral; Ristic, Bora; Price, Katherine; Madani, Kaveh

    2016-04-01

    The global issue of climate change has put pressure on governments to de-carbonise their energy portfolios by transitioning from the dominant use of fossil fuels energy to extensive use of renewable energies. The lack of renewable energy laws and credible targets and valid roadmaps for energy policies within the MENA region has let to ambitious and unrealistic renewable targets, where countries such as Djibouti and Morocco are aiming for 100% and 42% renewables respectively, by 2020, while Kuwait and Qatar are only aiming for 5% and 6% respectively. Nevertheless, this demonstrates the commitment and desirability of the members of the MENA region on increasing their share of renewables in their energy mix to reduce the greenhouse gas emissions of the region and minimise the unintended impacts of energy technologies on major natural resources through use of cost efficient technologies. The Relative Aggregate Footprint (RAF) of energy sources among the member states of the MENA region is assessed by applying the "System of Systems (SoS) Approach to Energy Sustainability Assessment" (Hadian and Madani, 2015). RAF demonstrates the efficiency of the overall resource-use of energy resources through creating a trade-off between carbon footprint, land footprint, water footprint, and economic cost. Using the resource availability of each member states, weights are assigned to the four criteria. This allows the evaluation of the desirability of energy sources with respect to regional resource availability and therefore, the efficiency of the overall resource-use of the energy portfolio of the MENA region is determined. This study has recognised the need for reform and radical changes within the MENA region's energy profile to make a significant contribution to the reduction of carbon emissions in order to use the resources in a sustainable way and increase the regional energy security of the member states across MENA. Reference: Hadian S, Madani K (2015) A System of Systems

  18. Toxicidade induzida pela terapêutica intermitente com Rifampicina: Análise de um caso clínico

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    Pilar Azevedo

    1997-01-01

    Full Text Available RESUMO: Descrevese o quadro clíoico de uma doente que desenvolveu uma reacção sistémica de hipersensibilidade grave a Rifampicina, objectivada por síndrome pseudo-gripal, anemia hemolítica, insuficiência renal aguda, choque e epidermólise bolhosa necrosante. Além da evolução clínica, salientase a dificuldade na formulação do diagnóstico e o apoio dado pelo laboratório na sua confirmação. SUMMARY: We repport the case of a patient who developed a severe drug-induced hypersensitivity sistemic reaction to Rifampicin, that included a flulike syndrome, severe hemolytic anaemia, renal failure, shock and toxic epidermal necrolysis.Besides the clinical evolution we consider the difficulties in clinical diagnosis and the role of the in vitro laboratory assays. Palavras-chave: Rifampicina, Efeitos ad versos, Imunologia, Toxicidade, Key-words: Rífampicin;Adverse effects, Immunology, Toxicity

  19. Cold stress effects on cardiomyocytes nuclear size in: light microscopic evaluation Efeitos do estresse pelo frio sobre o tamanho nuclear do cardiomiócito em ratos: avaliação por microscopia de luz

    Directory of Open Access Journals (Sweden)

    Adriano Meneghini

    2008-12-01

    Full Text Available INTRODUCTION: Total body induced hypothermia and myocardial cooling are effective methods regarding myocardial protection during heart surgery and ischemia. It is described in previous studies that extreme low temperature exposure causes mitochondrial cristae and myofilament disarrangement in cardiomyocytes, however, no investigation has analyzed the effects of cold stress on nuclear size of cardiomyocytes. OBJECTIVES: To evaluate the effects of acute cold stress exposure on the nuclear size of cardiomyocytes in rats. METHODS: The experimental study procedures were performed on 300-310g adult male Wistar rats. Rats (n=20 were divided into two groups: 1 Control (CON and; 2 Induced hypothermic (IH group. Animals of IH group were exposed during 4 hours once at a controlled temperature of - 8ºC. It was performed histological analysis of liver and adrenal gland to examine the stress condition of animals. Cardiomyocytes nucleus size were examined by three independent investigators with the same and standardized criteria and analyzed by Bartko's intra-class correlation coefficient (R>0.75 = positive concordance. Student's t test was applied. The significance level was set at PINTRODUÇÃO: Hipotermia corporal induzida e resfriamento do miocárdio são métodos efetivos em relação à proteção domiocárdio durante cirurgias cardíacas e isquemia. É descrito na literatura que a exposição a temperaturas extremamente baixas causa comprometimentos de miofilamentos e de cristas mitocondriais em cardiomiócitos, entretanto, nenhum estudo analisou os efeitos do estresse pelo frio no tamanho do núcleo dos cardiomiócios. OBJETIVOS: Analisar os efeitos do estresse agudo pelo frio sobre o tamanho do núcleo dos cardiomiócitos. MÉTODOS: O estudo foi realizado em ratos Wistar adultos, pesando 300-310g (n=20. Os ratos foram divididos em dois grupos: 1 Controle (CON e; 2 Hipotermia induzido (IH. Os animais do grupo IH foram expostos a uma temperatura

  20. Effect of an umuC-mutation on the SOS-response in E.coli cells exposed to UV-light and γ-radiation

    International Nuclear Information System (INIS)

    Komova, O.V.; Candiano, E.S.; Krasavin, E.A.

    1999-01-01

    Kinetics dependences of the SOS-induction in E.coli cells of wild type and deficient in umuC gene exposed to UV and γ-rays were analyzed. In the presence of UmuC protein SOS-induction was 3 -- 5.5 times lower and delayed for about 30 minutes after both UV and γ-rays. It was shown that the decrease of the SOS-induction in wild type cells irradiated by UV was due to more effective elimination of the photolesions from DNA by excision repair system. UmuCD-dependent inhibition of DNA replication was discussed as a possible mechanism allowing additional time for error-free repair. (author)

  1. RENOVANDO A TRADIÇÃO PELOS CAMINHOS DA INTERTEXTUALIDADE

    Directory of Open Access Journals (Sweden)

    Alba Olmi

    2007-08-01

    Full Text Available A revisão e a subversão dos contos de fadas e de outros textos canônicos realizadas por Angela Carter, Stevie Smith, Liz Lochhead e Anne Sexton, entre outras, se constituem na desconstrução/reconstrução da narrativa masculina através da qual é conferida uma nova forma literária, mas também uma nova voz à figura da mulher. Através da transposição diegética e da paródia, vão se modificando diversos elementos presentes na narrativa dos contos de fada tradicionais e de outros textos “canônicos”, o que produz uma transvalorização, isto é, uma transformação de natureza axiológica de seu primitivo horizonte cultural e de sentido. Essa revisão envolve também o nível lingüístico e as estruturas superficiais, porém sem nunca perder de vista a relação intertextual com os textos originais, questionando preconceitos e discriminação da figura feminina. Isso vem demonstrar que as mulheres estão criando uma literatura própria na qual histórias de mulheres são narradas do ponto de vista feminino.Transcendendo o sistema dicotômico-binário de gênero, que produziu relações seculares e históricas de poder, a escritura feminina reconstrói o conceito de diferença e de sujeito, provocando a ruptura do discurso hegemônico masculino, conferindo novo significado ao conceito de cultura. Observa-se, ainda, que o objetivo maior dessas re-escrituras não é simplesmente demolir uma tradição que perpetuou mitos e ideais patriarcais, mas evidenciar seus limites, suas lacunas e seus desvios, a fim de permitir que a tradição se renove, satisfazendo assim a dupla exigência de revisão e de continuidade em relação ao passado, pelos amplos caminhos da intertextualidade.

  2. Pomada orgânica natural ou solução salina isotônica no tratamento de feridas limpas induzidas em ratos

    Directory of Open Access Journals (Sweden)

    Rahal Sheila Canevese

    2001-01-01

    Full Text Available Foram utilizados 40 ratos Wistar, machos, com peso inicial médio de 225 gramas, separados em dois grupos. Após serem anestesiados com éter, foi induzida uma ferida limpa na região torácica dorsal cranial, mediante a utilização de molde metálico de 2,0 x 1,5cm. No grupo I, foi utilizada pomada contendo óleo de fígado de bacalhau, extrato de confrei, extrato cítrico, óxido de zinco e veículo (Creamex®; no grupo II, a ferida foi apenas limpa com solução salina isotônica. Para a avaliação histológica, foram submetidos à eutanásia cinco animais de cada grupo no 3º, 7º, 14º e 21º dia de pós-operatório. O grupo tratado com a pomada orgânica apresentou padrão cicatricial de qualidade superior em relação ao grupo tratado com salina isotônica, representado por formação de fibras colágenas, neoformação de vasos e reepitelização completa da epiderme.

  3. Encefalopatia induzida por cefepime: achados clínicos e eletroencefalográficos em sete pacientes Cefepime-induced encephalopathy: clinical and electroencephalographic features in seven patients

    Directory of Open Access Journals (Sweden)

    José Augusto Bragatti

    2005-03-01

    Full Text Available Cefepime, uma cefalosporina de quarta geração, com amplo espectro de ação, é um antibiótico largamente utilizado no tratamento de infecções graves em ambientes hospitalares. O registro de segurança deste fármaco é considerado favorável. Vários casos de encefalopatia grave, associada ao uso de cefepime, reversível, foram descritos recentemente. No presente artigo, descrevemos sete casos de encefalopatia induzida por cefepime, com achados eletroencefalográficos (EEG característicos, que apresentaram reversão do quadro com a suspensão da droga. As relações do padrão EEG encontrado nestes pacientes com estado epiléptico não-convulsivo são consideradas, bem como a possibilidade de enquadrar os pacientes estudados na entidade "encefalopatia epileptiforme".Cefepime, a fourth-generation cephalosporin, with large antibacterial spectrum, is a commonly used antibiotic for the treatment of serious hospitalar infections. Its security report is considered favourable. Recently, many cases of a severe and reversible cefepime-induced encephalopathy were described. In this paper, we report seven patients with reversible cefepime-induced encephalopathy, with a peculiar EEG pattern, characterized by semiperiodic diffuse triphasic waves. We discuss the EEG abnormalities found and their association with nonconvulsive status epilepticus.

  4. Effects of subinhibitory concentrations of antimicrobial agents on Escherichia coli O157:H7 Shiga toxin release and role of the SOS response.

    Science.gov (United States)

    Nassar, Farah J; Rahal, Elias A; Sabra, Ahmad; Matar, Ghassan M

    2013-09-01

    Treatment of Escherichia coli O157:H7 by certain antimicrobial agents often exacerbates the patient's condition by increasing either the release of preformed Shiga toxins (Stx) upon cell lysis or their production through the SOS response-triggered induction of Stx-producing prophages. Recommended subinhibitory concentrations (sub-MICs) of azithromycin (AZI), gentamicin (GEN), imipenem (IMI), and rifampicin (RIF) were evaluated in comparison to norfloxacin (NOR), an SOS-inducer, to assess the role of the SOS response in Stx release. Relative expression of recA (SOS-inducer), Q (late antitermination gene of Stx-producing prophage), stx1, and stx2 genes was assessed at two sub-MICs of the antimicrobials for two different strains of E. coli O157:H7 using reverse transcription-real-time polymerase chain reaction. Both strains at the two sub-MICs were also subjected to Western blotting for LexA protein expression and to reverse passive latex agglutination for Stx detection. For both strains at both sub-MICs, NOR and AZI caused SOS-induced Stx production (high recA, Q, and stx2 gene expression and high Stx2 production), so they should be avoided in E. coli O157:H7 treatment; however, sub-MICs of RIF and IMI induced Stx2 production in an SOS-independent manner except for one strain at the first twofold dilution below MIC of RIF where Stx2 production decreased. Moreover, GEN caused somewhat increased Stx2 production due to its mode of action rather than any effect on gene expression. The choice of antimicrobial therapy should rely on the antimicrobial mode of action, its concentration, and on the nature of the strain.

  5. Efeitos cardiorrespiratórios da buprenorfina em cães anestesiados pelo desfluorano Cardiorespiratory effects of buprenorphine in dogs anesthetized with desflurane

    Directory of Open Access Journals (Sweden)

    Almir Pereira de Souza

    2005-12-01

    Full Text Available Objetivou-se, com este estudo, avaliar os efeitos da buprenorfina sobre variáveis cardiovasculares e respiratórias em cães durante anestesia com desfluorano. Para tanto, foram utilizados 20 cães adultos, distribuídos em dois grupos (GB e GC. A anestesia foi induzida com propofol (8mg kg-1 IV e em seguida os animais foram intubados com sonda de Magill, a qual foi conectada ao aparelho de anestesia para administração de desfluorano (1,5 CAM. Após 30 minutos, foi aplicado no GB buprenorfina (0,02mg kg-1 e no GC solução de NaCl à 0,9% (0,05ml kg-1. Avaliaram-se: freqüências cardíaca e respiratória (FC e ¦; pressões arteriais sistólica, diastólica e média (PAS, PAD e PAM; débito cardíaco (DC; pressão venosa central (PVC; e as variáveis hemogasométricas pH, PaCO2, PaO2, HCO3, SatO2 e DB. As colheitas dos dados foram feitas aos 30 minutos após o início da administração do desfluorano (MO, 15 minutos após a administração do opióide ou placebo (M15, e a cada 15 minutos após Ml5 (M30, M45, M60 e M75. A avaliação estatística dos dados foi efetuada por meio de Análise de Perfil (P¦ e o pH tiveram reduções no GB, enquanto a PaCO2 esteve aumentada. Concluiu-se que a inclusão da buprenorfina durante anestesia inalatória pelo desfluorano determina discretas alterações cardiovasculares, bem-como potencializa a hipoventilação promovida pelo desfluorano, com a manifestação de hipercapnia, o que não contra-indica o seu uso em pacientes estáveis.The aim of this study was to evaluate the effects of buprenorphine on cardiovascular and respiratory variables in dogs anesthetized with desflurane. Twenty adult healthy male and female mongrel dogs were randomly distributed in two groups of ten animals each (GB and GC. The anesthetic induction was done using propofol (8mg kg-1, IV, and immediately, the dogs were intubated and submited to desflurane anesthesia administrated at 1.5 MAC. After 30 minutes of induction, animals

  6. Compras para a inovação: casos de inovações induzidas por clientes públicos.

    Directory of Open Access Journals (Sweden)

    Marina Figueiredo Moreira

    2012-10-01

    Full Text Available Este estudo se propõe a contribuir para a compreensão do processo de indução de inovações por clientes governamentais em atividades de serviços com a investigação de dez casos de inovações desenvolvidas por empresas prestadoras de serviços de software no Distrito Federal a clientes públicos. Adotou-se como opção teórica, para o exame da inovação em serviços, a abordagem integradora e investigou-se a premissa teórica escolhida para o direcionamento das compras governamentais para o fomento à inovação – compras para a inovação –, realizando-se uma leitura do modelo de análise chain-linked model desenvolvida em estudo anterior. A partir da perspectiva trazida pelo modelo, desenvolveu-se uma proposta de entendimento para a indução e a possível difusão de inovações em serviços de software com o uso de compras governamentais baseada em três etapas. Construiu-se um estudo descritivo, de natureza qualitativa e corte transversal. Dados foram coletados a partir de entrevistas semiestruturadas, nas quais os entrevistados apontaram a inovação mais significativa desenvolvida para um cliente governamental. As dez inovações selecionadas foram relatadas e analisadas de acordo com quatro critérios: descrição, momento de ocorrência, características e ganhos gerados para a empresa. As análises revelaram que as inovações surgiram como resposta a um requisito (ou conjunto de requisitos estabelecido por um cliente público e ocorreram em dois momentos: pré-venda e fase de desenvolvimento. As inovações confirmaram a premissa de indução trazida pelo modelo teórico adotado a partir de duas de suas trajetórias: caminho central da inovação, trajetória “c”, e conexões e feedback, trajetória “f”. Quanto às características, foram identificadas inovações radicais, incrementais e de melhoria. Os ganhos obtidos pelas empresas com as inovações confirmaram sua condição inovadora. A proposta de

  7. Inibição da guanilato ciclase pelo azul de metileno no choque circulatório causado por pancreatite aguda necrosante: uma palavra de cuidado embasada em modelo suíno

    Directory of Open Access Journals (Sweden)

    Caroline Floreoto Baldo

    Full Text Available OBJETIVO: estudar o uso terapêutico do bloqueio da guanilato ciclase pelo azul de metileno em um modelo experimental de pancreatite aguda grave em suínos. MÉTODOS: a pancreatite aguda necrotizante foi induzida em porcos anestesiados por infusão ductal pancreática retrógrada de 1ml/kg de taurocolato de sódio a 5% e 8U/kg de enteroquinase. Três grupos foram estudados (n=5: controle (C, pancreatite (PA, "bolus" de azul seguido por pancreatite (AM+PA. Os dados incluíram enzimas séricas e do líquido abdominal, variáveis hemodinâmicas, hemogasometria arterial, volume de líquido abdominal, marcadores inflamatórios plasmáticos, nitrito/nitrato e mieloperoxidase e malondialdeído plasmático. Aplicou-se a análise de variância seguida do pós-teste de Bonferroni (p<0,05. RESULTADOS: os valores de amilase e lipase foram três e dez vezes mais elevados no grupo PA. A atividade da mieloperoxidase foi 50% superior no grupo PA. Os dados hemodinâmicos indicaram choque hipovolêmico precoce seguido de choque cardiogênico. Observou-se grave translocação de líquidos para a cavidade peritoneal. A nitrito/nitrato plasmática permaneceu inalterada. O grupo AM+PA teve aumento de cinco vezes do mieloperoxidase em comparação com o grupo C. CONCLUSÕES: a utilização de azul de metileno em suínos com pancreatite não demonstrou efeitos significativos sobre variáveis hemodinâmicas e inflamatórias. Seu uso terapêutico na pancreatite necro-hemorrágica pode ser inadequado e extremo cuidado deve ser tomado dado o aumento da peroxidação lipídica evidenciado pelo aumento dos valores do malondialdeído.

  8. Cuidando de paciente com câncer de mama e osteonecrose mandibular induzida por bisfostonato: relato de experiência Cuidando de pacientes con cáncer de mama y osteonecrosis mandibular inducida por bisfosfonatos: relato de experiencia Providing care for patients with breast cancer and mandible ostheonecrosis induced by bisphosphonates: an experience report

    Directory of Open Access Journals (Sweden)

    Verônica Paula Torel de Moura

    2009-02-01

    Full Text Available OBJETIVO: Descrever as ações desenvolvidas pela enfermeira junto a uma paciente com câncer de mama e metástase óssea que apresentou necrose mandibular induzida pelo uso de bisfosfonato. RESULTADOS: As intervenções de enfermagem incluíram o ensino e reforço das orientações sobre higiene oral, destacando a escovação adequada, bochechos com solução antisséptica sem álcool, bem como sobre o controle da dor. CONCLUSÃO: Destaca-se a importância da atuação multiprofissional e da consulta de enfermagem no seguimento dessas pacientes para detecção precoce e controle dessa complicação.OBJETIVO: Describir las acciones de enfermería implementadas por la enfermera a una paciente con cáncer con metástasis ósea que presentó necrosis mandibular inducida por el uso de bisfosfonatos. RESULTADOS: Las intervenciones de enfermería incluyeron la enseñanza y el refuerzo de las orientaciones sobre la higiene oral, dando destaque al adecuado cepillado de los dientes, gárgaras con solución antiséptica sin alcohol y al control del dolor. CONCLUSIÓN: Es destacada el importancia de la actuación multiprofesional y de la consulta de enfermería en el seguimiento de esas pacientes, visando la detección temprana y el control de esa complicación.OBJECTIVE: To describe a nurse experience in providing care for a patient with cancer of the breast and bone metastasis who presented mandibular ostheonecrosis induced by the use of bisphosphonates. RESULTS: Nursing interventions included the re-enforcement of the guidelines for oral hygiene, highlighting the appropriate teeth-brushing technique, gargling with antiseptic solution without alcohol, as approach to pain management. CONCLUSION: There is a need for multidisciplinary and nursing consultations for early detection and control of potential complications.

  9. Electrochemical Genotoxicity Assay Based on a SOS/umu Test Using Hydrodynamic Voltammetry in a Droplet

    Science.gov (United States)

    Kuramitz, Hideki; Sazawa, Kazuto; Nanayama, Yasuaki; Hata, Noriko; Taguchi, Shigeru; Sugawara, Kazuharu; Fukushima, Masami

    2012-01-01

    The SOS/umu genotoxicity assay evaluates the primary DNA damage caused by chemicals from the β-galactosidase activity of S. typhimurium. One of the weaknesses of the common umu test system based on spectrophotometric detection is that it is unable to measure samples containing a high concentration of colored dissolved organic matters, sediment, and suspended solids. However, umu tests with electrochemical detection techniques prove to be a better strategy because it causes less interference, enables the analysis of turbid samples and allows detection even in small volumes without loss of sensitivity. Based on this understanding, we aim to develop a new umu test system with hydrodynamic chronoamperometry using a rotating disk electrode (RDE) in a microliter droplet. PAPG when used as a substrate is not electroactive at the potential at which PAP is oxidized to p-quinone imine (PQI), so the current response of chronoamperometry resulting from the oxidation of PAP to PQI is directly proportional to the enzymatic activity of S. typhimurium. This was achieved by performing genotoxicity tests for 2-(2-furyl)-3-(5-nitro-2-furyl)-acrylamide (AF-2) and 2-aminoanthracene (2-AA) as model genotoxic compounds. The results obtained in this study indicated that the signal detection in the genotoxicity assay based on hydrodynamic voltammetry was less influenced by the presence of colored components and sediment particles in the samples when compared to the usual colorimetric signal detection. The influence caused by the presence of humic acids (HAs) and artificial sediment on the genotoxic property of selected model compounds such as 4-nitroquinoline-N-oxide (4-NQO), 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX), 1,8-dinitropyrene (1,8-DNP) and 1-nitropyrene (1-NP) were also investigated. The results showed that the genotoxicity of 1-NP and MX changed in the presence of 10 mg·L−1 HAs. The genotoxicity of tested chemicals with a high hydrophobicity such as 1,8-DNP

  10. Multiple spinal nerve enlargement and SOS1 mutation: Further evidence of overlap between neurofibromatosis type 1 and Noonan phenotype.

    Science.gov (United States)

    Santoro, C; Giugliano, T; Melone, M A B; Cirillo, M; Schettino, C; Bernardo, P; Cirillo, G; Perrotta, S; Piluso, G

    2018-01-01

    Neurofibromatosis type 1 (NF1) has long been considered a well-defined, recognizable monogenic disorder, with neurofibromas constituting a pathognomonic sign. This dogma has been challenged by recent descriptions of patients with enlarged nerves or paraspinal tumors, suggesting that neurogenic tumors and hypertrophic neuropathy may be a complication of Noonan syndrome with multiple lentigines (NSML) or RASopathy phenotype. We describe a 15-year-old boy, whose mother previously received clinical diagnosis of NF1 due to presence of bilateral cervical and lumbar spinal lesions resembling plexiform neurofibromas and features suggestive of NS. NF1 molecular analysis was negative in the mother. The boy presented with Noonan features, multiple lentigines and pectus excavatum. Next-generation sequencing analysis of all RASopathy genes identified p.Ser548Arg missense mutation in SOS1 in the boy, confirmed in his mother. Brain and spinal magnetic resonance imaging scans were negative in the boy. No heart involvement or deafness was observed in proband or mother. This is the first report of a SOS1 mutation associated with hypertrophic neuropathy resembling plexiform neurofibromas, a rare complication in Noonan phenotypes with mutations in RASopathy genes. Our results highlight the overlap between RASopathies, suggesting that NF1 diagnostic criteria need rethinking. Genetic analysis of RASopathy genes should be considered when diagnosis is uncertain. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  11. Communication: A reduced scaling J-engine based reformulation of SOS-MP2 using graphics processing units

    Energy Technology Data Exchange (ETDEWEB)

    Maurer, S. A.; Kussmann, J.; Ochsenfeld, C., E-mail: Christian.Ochsenfeld@cup.uni-muenchen.de [Chair of Theoretical Chemistry, Department of Chemistry, University of Munich (LMU), Butenandtstr. 7, D-81377 München (Germany); Center for Integrated Protein Science (CIPSM) at the Department of Chemistry, University of Munich (LMU), Butenandtstr. 5–13, D-81377 München (Germany)

    2014-08-07

    We present a low-prefactor, cubically scaling scaled-opposite-spin second-order Møller-Plesset perturbation theory (SOS-MP2) method which is highly suitable for massively parallel architectures like graphics processing units (GPU). The scaling is reduced from O(N{sup 5}) to O(N{sup 3}) by a reformulation of the MP2-expression in the atomic orbital basis via Laplace transformation and the resolution-of-the-identity (RI) approximation of the integrals in combination with efficient sparse algebra for the 3-center integral transformation. In contrast to previous works that employ GPUs for post Hartree-Fock calculations, we do not simply employ GPU-based linear algebra libraries to accelerate the conventional algorithm. Instead, our reformulation allows to replace the rate-determining contraction step with a modified J-engine algorithm, that has been proven to be highly efficient on GPUs. Thus, our SOS-MP2 scheme enables us to treat large molecular systems in an accurate and efficient manner on a single GPU-server.

  12. Communication: A reduced scaling J-engine based reformulation of SOS-MP2 using graphics processing units.

    Science.gov (United States)

    Maurer, S A; Kussmann, J; Ochsenfeld, C

    2014-08-07

    We present a low-prefactor, cubically scaling scaled-opposite-spin second-order Møller-Plesset perturbation theory (SOS-MP2) method which is highly suitable for massively parallel architectures like graphics processing units (GPU). The scaling is reduced from O(N⁵) to O(N³) by a reformulation of the MP2-expression in the atomic orbital basis via Laplace transformation and the resolution-of-the-identity (RI) approximation of the integrals in combination with efficient sparse algebra for the 3-center integral transformation. In contrast to previous works that employ GPUs for post Hartree-Fock calculations, we do not simply employ GPU-based linear algebra libraries to accelerate the conventional algorithm. Instead, our reformulation allows to replace the rate-determining contraction step with a modified J-engine algorithm, that has been proven to be highly efficient on GPUs. Thus, our SOS-MP2 scheme enables us to treat large molecular systems in an accurate and efficient manner on a single GPU-server.

  13. Evidence for Different Disk Mass Distributions between Early- and Late-type Be Stars in the BeSOS Survey

    Energy Technology Data Exchange (ETDEWEB)

    Arcos, C.; Kanaan, S.; Curé, M. [Instituto de Física y Astronomía, Facultad de Ciencias, Universidad de Valparaíso. Av. Gran Bretana 1111, Valparaíso (Chile); Jones, C. E.; Sigut, T. A. A. [Department of Physics and Astronomy, The University of Western Ontario, London, ON N6A 3K7 (Canada)

    2017-06-10

    The circumstellar disk density distributions for a sample of 63 Be southern stars from the BeSOS survey were found by modeling their H α emission line profiles. These disk densities were used to compute disk masses and disk angular momenta for the sample. Average values for the disk mass are 3.4 × 10{sup −9} and 9.5 × 10{sup −10} M {sub ⋆} for early (B0–B3) and late (B4–B9) spectral types, respectively. We also find that the range of disk angular momentum relative to the star is (150–200) J {sub ⋆}/ M {sub ⋆} and (100–150) J {sub ⋆}/ M {sub ⋆}, again for early- and late-type Be stars, respectively. The distributions of the disk mass and disk angular momentum are different between early- and late-type Be stars at a 1% level of significance. Finally, we construct the disk mass distribution for the BeSOS sample as a function of spectral type and compare it to the predictions of stellar evolutionary models with rapid rotation. The observed disk masses are typically larger than the theoretical predictions, although the observed spread in disk masses is typically large.

  14. DnaC inactivation in Escherichia coli K-12 induces the SOS response and expression of nucleotide biosynthesis genes

    DEFF Research Database (Denmark)

    Løbner-Olesen, Anders; Slominska-Wojewodzka, Monika; Hansen, Flemming G.

    2008-01-01

    Background: Initiation of chromosome replication in E. coli requires the DnaA and DnaC proteins and conditionally-lethal dnaA and dnaC mutants are often used to synchronize cell populations. Methodology/Principal Findings: DNA microarrays were used to measure mRNA steady-state levels in initiatio......C genes was increased at the non-permissive temperature in the respective mutant strains indicating auto-regulation of both genes. Induction of the SOS regulon was observed in dnaC2 cells at 38 degrees C and 42 degrees C. Flow cytometric analysis revealed that dnaC2 mutant cells at non......-permissive temperature had completed the early stages of chromosome replication initiation. Conclusion/Significance: We suggest that in dnaC2 cells the SOS response is triggered by persistent open-complex formation at oriC and/or by arrested forks that require DnaC for replication restart....

  15. Blocking the RecA activity and SOS-response in bacteria with a short α-helical peptide.

    Science.gov (United States)

    Yakimov, Alexander; Pobegalov, Georgii; Bakhlanova, Irina; Khodorkovskii, Mikhail; Petukhov, Michael; Baitin, Dmitry

    2017-09-19

    The RecX protein, a very active natural RecA protein inhibitor, can completely disassemble RecA filaments at nanomolar concentrations that are two to three orders of magnitude lower than that of RecA protein. Based on the structure of RecX protein complex with the presynaptic RecA filament, we designed a short first in class α-helical peptide that both inhibits RecA protein activities in vitro and blocks the bacterial SOS-response in vivo. The peptide was designed using SEQOPT, a novel method for global sequence optimization of protein α-helices. SEQOPT produces artificial peptide sequences containing only 20 natural amino acids with the maximum possible conformational stability at a given pH, ionic strength, temperature, peptide solubility. It also accounts for restrictions due to known amino acid residues involved in stabilization of protein complexes under consideration. The results indicate that a few key intermolecular interactions inside the RecA protein presynaptic complex are enough to reproduce the main features of the RecX protein mechanism of action. Since the SOS-response provides a major mechanism of bacterial adaptation to antibiotics, these results open new ways for the development of antibiotic co-therapy that would not cause bacterial resistance. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  16. Induction of UV-resistant DNA replication in Escherichia coli: Induced stable DNA replication as an SOS function

    International Nuclear Information System (INIS)

    Kogoma, T.; Torrey, T.A.; Connaughton, M.J.

    1979-01-01

    The striking similarity between the treatments that induce SOS functions and those that result in stable DNA replication (continuous DNA replication in the absence of protein synthesis) prompted us to examine the possibility of stable DNA replication being a recA + lexA + -dependent SOS function. In addition to the treatments previously reported, ultraviolet (UV) irradiation or treatment with mitomycin C was also found to induce stable DNA replication. The thermal treatment of tif-1 strains did not result in detectable levels of stable DNA replication, but nalidixic acid readily induced the activity in these strains. The induction of stable DNA replication with nalidixic acid was severely suppressed in tif-1 lex A mutant strains. The inhibitory activity of lexA3 was negated by the presence of the spr-5l mutation, an intragenic suppressor of lexA3. Induced stable DNA replication was found to be considerably more resistant to UV irradiation than normal replication both in a uvr A6 strain and a uvr + strain. The UV-resistant replication occurred mostly in the semiconservative manner. The possible roles of stable DNA replication in repair of damaged DNA are discussed. (orig.)

  17. A Network of Resistances against a Multiple Crisis. SOS Rosarno and the Experimentation of Socio-Economic Alternative Models

    Directory of Open Access Journals (Sweden)

    Federico Oliveri

    2015-07-01

    Full Text Available SOS Rosarno was launched in 2011 by a group of small farmers and activists based in the Gioia Tauro Plain, Calabria, Southern Italy. The idea was to sell organic citrus fruits through short self-organized supply chains, essentially based on Solidarity Purchase Groups, in order to allow producers to pay migrant workers according to the law, to receive a fair remuneration, to guarantee healthy and affordable food to consumers, to protect the integrity of the environment. This paper aims to reconstruct the ideological frame and the genealogy, the organization and the practices, the impact and the limits of SOS Rosarno, drawing mainly on the political documents produced by the association and in-depth interviews with its diverse members. It clarifies, on one side, the strategies of alternative economy and the new social alliances implemented in order to challenge those conditions which impoverish small producers and let migrant farmworkers be exploited and become the target of racism in many Italian countrysides. It explores, on the other side, the development of a new peasant civilization as alternative to the current economic and environmental crises, in terms of de-commodification of nature and labour, construction of a convivial democratic society, transition from monoculture to food sovereignty.

  18. SOS1 and PTPN11 mutations in five cases of Noonan syndrome with multiple giant cell lesions.

    Science.gov (United States)

    Beneteau, Claire; Cavé, Hélène; Moncla, Anne; Dorison, Nathalie; Munnich, Arnold; Verloes, Alain; Leheup, Bruno

    2009-10-01

    We report five cases of multiple giant cell lesions in patients with typical Noonan syndrome. Such association has frequently been referred to as Noonan-like/multiple giant cell (NL/MGCL) syndrome before the molecular definition of Noonan syndrome. Two patients show mutations in PTPN11 (p.Tyr62Asp and p.Asn308Asp) and three in SOS1 (p.Arg552Ser and p.Arg552Thr). The latter are the first SOS1 mutations reported outside PTPN11 in NL/MGCL syndrome. MGCL lesions were observed in jaws ('cherubism') and joints ('pigmented villonodular synovitis'). We show through those patients that both types of MGCL are not PTPN11-specific, but rather represent a low penetrant (or perhaps overlooked) complication of the dysregulated RAS/MAPK signaling pathway. We recommend discarding NL/MGCL syndrome from the nosology, as this presentation is neither gene-nor allele-specific of Noonan syndrome; these patients should be described as Noonan syndrome with MGCL (of the mandible, the long bone...). The term cherubism should be used only when multiple giant cell lesions occur without any other clinical and molecular evidence of Noonan syndrome, with or without mutations of the SH3BP2 gene.

  19. Comparative study of SOS response induced by hydrogen peroxide in the absence or presence of iron ions, in Escherichia coli

    International Nuclear Information System (INIS)

    Almeida, Carlos Eduardo Bonacossa de

    1994-01-01

    The H 2 O 2 is an reactive oxygen specie that arises from cell respiration process. It may cause deleterious effects on cell, by reacting with transition metals like iron. In this way it yields free radicals that are able to damage organic molecules, mainly DNA. Recent works have suggested that in the absence of Fe ions H 2 O 2 still damages Escherichia coli DNA. This work presents a comparative analysis of cell SOS responses to DNA damage in Escherichia coli and Salmonella typhimurium mutants pretreated or not with a Fe 2+ ion chelator (dipyridyl) and then treated with H 2 O 2 . The systems analysed were the lysogenic induction, Weigle reactivation, mutagenesis and cell inactivation curves. The cell inactivation curves were themselves distinct, in relation to both treatments. The increased sensitivity found in the lexA1 and recA13 mutants, when treated with dipyridyl and H 2 O 2 , suggests an important role of SOS response in repairing the lesions caused by this treatment. The profiles of the lysogenic induction and mutagenesis curves were also distinct in both treatments. The results of Weigle reactivation suggest that the products of uvrA and lexA genes have an important role in UV-damaged bacteriophage DNA repair, when dipyridyl-pretreated cells are treated with H 2 O 2 . All the results suggest that Fe-independent lesions produced by H 2 O 2 are different from the ones produced in the presence of this ion. (author)

  20. Contribuições para interação pelo olhar com teclados virtuais

    OpenAIRE

    Antonio Díaz Tula

    2015-01-01

    A presente tese de doutorado insere-se na área de interação pelo olhar. A interação pelo olhar é uma forma de comunicação com o computador utilizando os movimentos oculares do usuário. Pessoas com deficiência física, que não conseguem usar dispositivos convencionais como um teclado e um mouse de computador, podem se beneficiar da interação pelo olhar para se comunicarem e se inserirem na sociedade. Para isso a entrada de texto ou digitação pelo olhar é um recurso importante e assunto principa...

  1. Efeito do desajuste marginal, tipo de soldagem e material do parafuso sobre a força de destorque de parafusos protéticos e tensões induzidas aos pilares

    OpenAIRE

    Sabrina Alessandra Rodrigues

    2012-01-01

    Resumo: O objetivo neste estudo foi analisar as tensões induzidas aos análogos de pilares protéticos, utilizando-se strain gauges; e avaliar a força de destorque de parafusos protéticos (ouro e titânio) em infraestruturas fundidas em titânio comercialmente puro (Ti cp), antes e após os procedimentos de soldagem laser e TIG (tungsten inert gas). Foram confeccionadas vinte infraestruturas fundidas em titânio comercialmente puro (Ti cp), simulando uma prótese fixa de três elementos; vinte modelo...

  2. Incidência e prevalência de perda auditiva induzida por ruído em trabalhadores de uma indústria metalúrgica, Manaus - AM, Brasil

    Directory of Open Access Journals (Sweden)

    Ana Cristina Furtado de Carvalho Régis

    2014-10-01

    Full Text Available OBJETIVO: estimar a incidência e a prevalência de déficit auditivo sugestivo de Perda Auditiva Induzida por Ruído e sua associação com idade e tempo de serviço em trabalhadores de uma indústria metalúrgica do pólo industrial de Manaus.MÉTODOS: estudo transversal descritivo em trabalhadores que se submeteram a exame audiométrico periódico no ano de 2012, totalizando 1499 sujeitos. Para estimativa da incidência foram selecionadas audiometrias de 763 trabalhadores com audição dentro da normalidade no exame de referência e comparados com exame atual. Realizou-se análise estatística por meio de medidas de tendência central, dispersão e distribuições de frequência. Para verificação de diferenças estatisticamente significantes utilizou-se o teste qui-quadrado, com nível de significância (p≤0,05.RESULTADOS: a prevalência de perda auditiva foi de 44,23% sendo 28,89% sugestivo de PAIR. Houve maior prevalência de perda auditiva nos trabalhadores com faixa etária acima de 45 anos e com tempo de serviço superior a 21 anos. Apenas 11,1% dos trabalhadores acima dos 21 anos de serviço apresentaram audição normal, e 61,9% perda auditiva sugestiva de Perda Auditiva Induzida por Ruído. A classificação de Não Sugestivo de Perda Auditiva Induzida por Ruído permanece estável nos indivíduos abaixo de 20 anos de exposição laboral (14,9% e nas pessoas expostas com mais de 20 anos aumenta para 27%. A incidência de perda auditiva foi de 28% e desse total 19,7% sugestiva de Perda Auditiva Induzida por Ruído. Houve maior prevalência de perda auditiva grau leve.Conclusão:a prevalência e a incidência de perda auditiva aumentaram com a idade e tempo de serviço. As empresas devem se empenhar na implementação do Programa de Conservação Auditiva a fim de minimizar essas perdas.

  3. Towards Developing Science of Survival (SOS) Pamphlets for "Typhoon, Flashflood, Storm Surge and Tsunami" and for "Earthquakes and Their Aftermath": A Pilot Study

    Science.gov (United States)

    Nivera, Gladys; Camacho, Vic Marie; Sia, Shila Rose; Avilla, Ruel; Butron, Benilda; Fernandez, Eisha Vienna; Pastor, Crist John; Reyes, Allan; Palomar, Brando

    2017-01-01

    The catastrophic devastation from recent natural calamities in the Philippines such as Typhoon Yolanda and Central Visayas earthquake in 2013 had made disaster preparedness a primary concern in the country. Prompted by the critical need to use science to save lives, this study developed Science of Survival (SOS) pamphlets titled "When the…

  4. Investigation of potential genotoxic activity using the SOS Chromotest for real paracetamol wastewater and the wastewater treated by the Fenton process.

    Science.gov (United States)

    Kocak, Emel

    2015-01-01

    The potential genotoxic activity associated with high strength real paracetamol (PCT) wastewater (COD = 40,000 mg/L, TOC = 12,000 mg/L, BOD5 = 19,320 mg/L) from a large-scale drug-producing plant in the Marmara Region, was investigated in pre- and post- treated wastewater by the Fenton process (COD = 2,920 mg/L, TOC = 880 mg/L; BOD5 = 870 mg/L). The SOS Chromotest, which is based on Escherichia coli PQ37 activities, was used for the assessment of genotoxicity. The corrected induction factors (CIF) values used as quantitative measurements of the genotoxic activity were obtained from a total of four different dilutions (100, 50, 6.25, and 0.078 % v/v.) for two samples, in triplicate, to detect potentially genotoxic activities with the SOS Chromotest. The results of the SOS Chromotest demonstrated CIFmax value of 1.24, indicating that the PCT effluent (non-treated) is genotoxic. The results of the SOS Chromotest showed an CIFmax value of 1.72, indicating that the wastewater treated by Fenton process is genotoxic. The findings of this study clearly reveal that the PCT wastewater (non-treated) samples have a potentially hazardous impact on the aquatic environment before treatment, and in the wastewater that was treated by the Fenton process, genotoxicity generally increased.

  5. Comparative study of SOS2 and a novel PMP3-1 gene expression in two sunflower (Helianthus annuus L.) lines differing in salt tolerance.

    Science.gov (United States)

    Saadia, Mubshara; Jamil, Amer; Ashraf, Muhammad; Akram, Nudrat Aisha

    2013-06-01

    Gene expression pattern of two important regulatory proteins, salt overly sensitive 2 (SOS2) and plasma membrane protein 3-1 (PMP3-1), involved in ion homeostasis, was analyzed in two salinity-contrasting sunflower (Helianthus annuus L.) lines, Hysun-38 (salt tolerant) and S-278 (moderately salt tolerant). The pattern was studied at selected time intervals (24 h) under 150 mM NaCl treatment. Using reverse transcription PCR, SOS2 gene fragment was obtained from young leaf and root tissues of opposing lines while that for PMP3-1 was obtained only from young root tissues. Both tolerant and moderately tolerant lines showed a gradual increase in SOS2 expression in sunflower root tissues. Leaf tissues showed the gradually increasing pattern of SOS2 expression in tolerant plants as compared to that for moderately tolerant ones that showed a relatively lower level of expression for this gene. We found the highest level of PMP 3-1 expression in the roots of tolerant sunflower line at 6 and 12 h postsalinity treatment. The moderately tolerant line showed higher expression of PMP3-1 at 12 and 24 h after salt treatment. Overall, the expression of genes for both the regulator proteins varied significantly in the two sunflower lines differing in salinity tolerance.

  6. Complexity of the ultraviolet mutation frequency response curve in Escherichia coli B/r: SOS induction, one-lesion and two-lesion mutagenesis

    International Nuclear Information System (INIS)

    Doudney, C.O.

    1976-01-01

    Three distinct sections of the ultraviolet mutation frequency response (MFR) curve toward tryptophan prototrophy have been demonstrated in Escherichia coli B/r WP2 trp thy and its uvrA derivative in log-phase growth in minimal medium. The initial section, which appears fluence-squared, may reflect the necessity, if mutation is to result, for induction of two lesions, one located within the potentially mutated genetic locus and the other damaging deoxyribonucleic acid replication and resulting in induction of the error-prone SOS repair function. A second linear section is ascribed to the continued induction, after exposure above that sufficient for complete SOS expression, of isolated lesions which lead to mutation in potentially mutated loci. The third section demonstrates an increased rate of mutagenesis and suggests the induction of two lesions in proximity which result in additional mutations. Split-exposure studies support the inducible nature of the SOS function and suggest that mutation frequency decline (MFD) is due to excision resulting from or related to the prevention of SOS induction by inhibition of protein synthesis. Preirradiation tryptophan starvation of the uvr + strain for 30 min decreases MFR in the first and second sections of the curve. Reduction of MFR in the third section requires more prestarvation time and is blocked by nalidixic acid. The decreased MFR of the first and second sections is ascribed to promotion of postirradiation MFD based on excision and that of the third section to completion of the chromosome during the prestarvation period

  7. RUNX1 positively regulates the ErbB2/HER2 signaling pathway through modulating SOS1 expression in gastric cancer cells.

    Science.gov (United States)

    Mitsuda, Yoshihide; Morita, Ken; Kashiwazaki, Gengo; Taniguchi, Junichi; Bando, Toshikazu; Obara, Moeka; Hirata, Masahiro; Kataoka, Tatsuki R; Muto, Manabu; Kaneda, Yasufumi; Nakahata, Tatsutoshi; Liu, Pu Paul; Adachi, Souichi; Sugiyama, Hiroshi; Kamikubo, Yasuhiko

    2018-04-23

    The dual function of runt-related transcriptional factor 1 (RUNX1) as an oncogene or oncosuppressor has been extensively studied in various malignancies, yet its role in gastric cancer remains elusive. Up-regulation of the ErbB2/HER2 signaling pathway is frequently-encountered in gastric cancer and contributes to the maintenance of these cancer cells. This signaling cascade is partly mediated by son of sevenless homolog (SOS) family, which function as adaptor proteins in the RTK cascades. Herein we report that RUNX1 regulates the ErbB2/HER2 signaling pathway in gastric cancer cells through transactivating SOS1 expression, rendering itself an ideal target in anti-tumor strategy toward this cancer. Mechanistically, RUNX1 interacts with the RUNX1 binding DNA sequence located in SOS1 promoter and positively regulates it. Knockdown of RUNX1 led to the decreased expression of SOS1 as well as dephosphorylation of ErbB2/HER2, subsequently suppressed the proliferation of gastric cancer cells. We also found that our novel RUNX inhibitor (Chb-M') consistently led to the deactivation of the ErbB2/HER2 signaling pathway and was effective against several gastric cancer cell lines. Taken together, our work identified a novel interaction of RUNX1 and the ErbB2/HER2 signaling pathway in gastric cancer, which can potentially be exploited in the management of this malignancy.

  8. Predisposition to metabolic acidosis induced by topiramate Predisposição a acidose metabólica induzida por topiramato

    Directory of Open Access Journals (Sweden)

    MARIA AUGUSTA MONTENEGRO

    2000-12-01

    Full Text Available RATIONALE: Metabolic acidosis induced by topiramate is a well documented but infrequent adverse event. The objective was to demonstrate the lowering of carbon dioxide serum levels, which is usually asymptomatic but may facilitate the occurrence of metabolic acidosis in patients using topiramate. METHODS: We evaluated, prospectively, the carbon dioxide serum levels of 18 patients seen at the epilepsy clinic of our university hospital, before and 3 months after introducing topiramate. RESULTS: Five patients were female and 13 were male, age ranging from 2 to 16 years old (mean=9.3. Carbon dioxide mean serum levels were 25 and 21.2 mmol/L (normal = 22 to 30, before and 3 months after introducing topiramate, respectively. Dose ranged from 2.08 to 11.76 mg/kg/day (mean=6.7mg/kg/day. Adverse events were anorexia, nausea and somnolence. CONCLUSION: We conclude that the lowering of carbon dioxide serum levels induced by topiramate is mostly asymptomatic, but may facilitate the occurrence of metabolic acidosis. Since patients in use of topiramate have refractory epilepsy, they may need epilepsy surgery, and must be carefully monitored for the risk of metabolic acidosis during surgery.INTRODUÇÃO: Acidose metabólica induzida por topiramato é evento adverso pouco frequente, mas bem documentado. Nosso objetivo foi demonstrar a diminuição dos níveis de dióxido de carbono, muitas vezes assintomática, mas que pode predispor ao aparecimento de acidose metabólica. MATERIAL E MÉTODO: Avaliamos prospectivamente os níveis de dióxido de carbono de 18 pacientes acompanhados no ambulatório de epilepsia infantil da UNICAMP, antes e 3 meses após o início do uso de topiramato. RESULTADOS: Foram avaliados 18 pacientes com idade entre 2 e 16 anos (média = 9,3 anos. Cinco pacientes eram do sexo feminino e 13, do sexo masculino. Os níveis médios de dióxido de carbono antes e após o uso de topiramato foram 25 e 21,2 mmol/L (normal = 22 a 30

  9. Disfunção ventricular esquerda transitória por cardiomiopatia induzida por estresse Transient left ventricular dysfunction due to stress-induced cardiomyopathy

    Directory of Open Access Journals (Sweden)

    Marcus Vinicius Simões

    2007-10-01

    Full Text Available Apresenta-se o caso de uma paciente de 71 anos que preencheu os critérios diagnósticos para cardiomiopatia induzida por estresse que foi desencadeada por intenso estresse emocional após atropelamento por bicicleta. O quadro clínico mimetizou o infarto agudo do miocárdio, manifestando-se com dor precordial, supradesnivelamento do segmento ST, seguido por ondas T profundas e prolongamento do intervalo QT, elevação discreta de enzimas cardíacas e cursando com disfunção sistólica apical do ventrículo esquerdo e hipercinesia das porções basais (conferindo o aspecto de "abaloamento apical", mas na ausência de obstrução coronariana subepicárdica. A função ventricular normalizou-se após a segunda semana de evolução.The case presented here is of a 71-yr-old female patient who met the diagnostic criteria for stress-induced cardiomyopathy, which was triggered by intense emotional stress after being hit by a bicycle. The clinical picture mimicked that of an acute myocardial infarction, manifesting as precordial pain, ST-segment depression followed by deep negative T waves and prolonging of the QT interval, slight increase in cardiac enzymes and coursing with transient apical ballooning of the left ventricle and hyperkinesis of the basal walls (conferring the aspect of "apical ballooning", although in the absence of subepicardial coronary obstruction. Ventricular function normalized after the second week of clinical evolution.

  10. Structural analyses of polymorphic transitions of sn-1, 3-distearoyl-2-oleoylglycerol (SOS) and sn-1, 3-dioleoyl-2-stearoylglycerol (OSO): assessment on steric hindrance of unsaturated and saturated acyl chain interactions.

    Science.gov (United States)

    Yano, J; Sato, K; Kaneko, F; Small, D M; Kodali, D R

    1999-01-01

    Polymorphic transformations in two saturated-unsaturated mixed acid triacylglycerols, SOS (sn -1,3-distearoyl-2-oleoylglycerol) and OSO (sn -1,3-dioleoyl-2-stearoylglycerol), have been studied by FT-IR spectroscopy using deuterated specimens in which stearoyl chains are fully deuterated. A reversible phase transition between sub alpha and alpha and a series of irreversible transitions (alpha-->gamma-->beta'-->beta (beta2, beta1) for SOS and alpha-->beta'-->beta for OSO) were studied with an emphasis on the conformational ordering process of stearoyl and oleoyl chains. The alpha-->sub alpha reversible transition was due to the orientational change of stearoyl chains in the lateral directions from the hexagonal subcell to a perpendicularly packed one. As the first stage of the series of irreversible transitions from alpha to beta, the conformational ordering of saturated chains took place in the alpha-->gamma transition of SOS and in the alpha-->beta' transition of OSO; one stearoyl chain in SOS and OSO takes the all-trans conformation and the second stearoyl chain in SOS takes the bent conformation like those observed in the most stable beta-type. As the final stage, the ordering of unsaturated chains occurred in the beta'-->beta transition both for SOS and OSO. A conversion in the layered structure from bilayer to trilayer was also accompanied by the conformational ordering in the alpha-->gamma transition of SOS and in the beta'-->beta transition of OSO.

  11. Proteus - A Free and Open Source Sensor Observation Service (SOS) Client

    Science.gov (United States)

    Henriksson, J.; Satapathy, G.; Bermudez, L. E.

    2013-12-01

    The Earth's 'electronic skin' is becoming ever more sophisticated with a growing number of sensors measuring everything from seawater salinity levels to atmospheric pressure. To further the scientific application of this data collection effort, it is important to make the data easily available to anyone who wants to use it. Making Earth Science data readily available will allow the data to be used in new and potentially groundbreaking ways. The US National Science and Technology Council made this clear in its most recent National Strategy for Civil Earth Observations report, when it remarked that Earth observations 'are often found to be useful for additional purposes not foreseen during the development of the observation system'. On the road to this goal the Open Geospatial Consortium (OGC) is defining uniform data formats and service interfaces to facilitate the discovery and access of sensor data. This is being done through the Sensor Web Enablement (SWE) stack of standards, which include the Sensor Observation Service (SOS), Sensor Model Language (SensorML), Observations & Measurements (O&M) and Catalog Service for the Web (CSW). End-users do not have to use these standards directly, but can use smart tools that leverage and implement them. We have developed such a tool named Proteus. Proteus is an open-source sensor data discovery client. The goal of Proteus is to be a general-purpose client that can be used by anyone for discovering and accessing sensor data via OGC-based services. Proteus is a desktop client and supports a straightforward workflow for finding sensor data. The workflow takes the user through the process of selecting appropriate services, bounding boxes, observed properties, time periods and other search facets. NASA World Wind is used to display the matching sensor offerings on a map. Data from any sensor offering can be previewed in a time series. The user can download data from a single sensor offering, or download data in bulk from all

  12. The role of complementary and alternative medicine (CAM) routines and rituals in men with cancer and their significant others (SOs): a qualitative investigation.

    Science.gov (United States)

    Klafke, Nadja; Eliott, Jaklin A; Olver, Ian N; Wittert, Gary A

    2014-05-01

    Complementary and alternative medicine (CAM) is frequently used in cancer patients, often with contribution of the significant others (SOs), but without consultation of healthcare professionals. This research explored how cancer patients integrate and maintain CAM use in their everyday life, and how SOs are involved in it. In this qualitative study, male participants were selected from a preceding Australian survey on CAM use in men with cancer (94 % response rate and 86 % consent rate for follow-up interview). Semistructured interviews were conducted with 26 men and 24 SOs until data saturation was reached. Interview transcripts were coded and analyzed thematically, thereby paying close attention to participants' language in use. A major theme associated with high CAM use was "CAM routines and rituals," as it was identified that men with cancer practiced CAM as (1) functional routines, (2) meaningful rituals, and (3) mental/spiritual routines or/and rituals. Regular CAM use was associated with intrapersonal and interpersonal benefits: CAM routines provided men with certainty and control, and CAM rituals functioned for cancer patients and their SOs as a means to create meaning, thereby working to counter fear and uncertainty consequent upon a diagnosis of cancer. SOs contributed most to men's uptake and maintenance of dietary-based CAM in ritualistic form resulting in interpersonal bonding and enhanced closeness. CAM routines and rituals constitute key elements in cancer patients' regular and satisfied CAM use, and they promote familial strengthening. Clinicians and physicians can convey these benefits to patient consultations, further promoting the safe and effective use of CAM.

  13. Differential expression of SOS genes in an E. coli mutant producing unstable lexA protein enhances excision repair but inhibits mutagenesis

    International Nuclear Information System (INIS)

    Peterson, K.R.; Ganesan, A.K.; Mount, D.W.; Stanford Univ., CA)

    1986-01-01

    The SOS response is displayed following treatments which damage DNA or inhibit DNA replication. Two associated activities include enhanced capacity for DNA repair resulting from derepression of the recA, uvrA, uvrB and uvrD genes and increased mutagenesis due to derepression of recA, umuC and umuD. These changes are the consequence of the derepression of at least seventeen unlinked operons negatively regulated by LexA repressor. Following treatments that induce the SOS response, a signal molecule interacts with RecA protein, converting it to an activated form. Activated RecA protein facilitates the proteolytic cleavage of LexA repressor, which results in derepression of the regulon. The cell then enters a new physiological state during which time DNA repair processes are augmented. The lexA41 mutant of E. coli is a uv-resistant derivative of another mutant, lexA3, which produces a repressor that is not cleaved following inducing treatments. The resultant protein is unstable. Lac operon fusions to most of the genes in the SOS regulon were used to show that the various damage-inducible genes were derepressed to different extents. uvrA, B, and D were almost fully derepressed. Consistent with this finding, the rate of removal of T4 endonuclease V-sensitive sites was more rapid in the uv-irradiated lexA41 mutant than in normal cells, suggesting a more active excision repair system. We propose that the instability of the LexA41 protein reduces the intracellular concentration of repressor to a level that allows a high level of excision repair. The additional observation that SOS mutagenesis was only weakly induced in a lexA41 uvrA - mutant implies that the mutant protein partially represses one or more genes whose products promote SOS mutagenesis. 17 refs., 4 figs., 1 tab

  14. Ferruleless coupled-cavity traveling-wave tube cold-test characteristics simulated with micro-SOS

    Science.gov (United States)

    Schroeder, Dana L.; Wilson, Jeffrey D.

    1993-01-01

    The three-dimensional, electromagnetic circuit analysis code, Micro-SOS, can be used to reduce expensive and time consuming experimental 'cold-testing' of traveling-wave tube (TWT) circuits. The frequency-phase dispersion and beam interaction impedance characteristics of a ferruleless coupled-cavity traveling-wave tube slow-wave circuit were simulated using the code. Computer results agree closely with experimental data. Variations in the cavity geometry dimensions of period length and gap-to-period ratio were modeled. These variations can be used in velocity taper designs to reduce the radiofrequency (RF) phase velocity in synchronism with the decelerating electron beam. Such circuit designs can result in enhanced TWT power and efficiency.

  15. Paraísos artificiales. La imagen drogada en la pintura europea del entresiglos XIX-XX

    OpenAIRE

    Barrón, Sofía

    2015-01-01

    Paraísos artificiales. La imagen drogada en la pintura europea del entresiglos XIX-XX. La presente investigación recopila y analiza la imagen pictórica drogada en el entresiglos XIX-XX europeo, prestando especial atención al caso español, desde la exposición descriptiva; un trabajo elaborado con voluntad de catálogo. Las conclusiones teóricas dan cuenta no sólo de que el leitmotiv tóxico puede convertirse en una temática cambiosecular en sí misma, también pone de manifiesto como el uso y ...

  16. New data on the epigraphy of Campo Real/Fillera (Sos del Rey Católico-Sangüesa

    Directory of Open Access Journals (Sweden)

    Javier ANDREU PINTADO

    2010-07-01

    Full Text Available The aim of this paper is to present the conclusions of two new epigraphic autopsies over two latin inscriptions –unpublished the first one, the second already known but here for the first time reviewed– found in the archaeological site of Campo Real/Fillera (Sos del Rey Católico-Sangüesa, in the northeast part of ancient Vascones’ territory. Some new archaeological materials related with the history of the monumentalization process of this ancient ciuitas are also presented: specially an spectacular funerary osteotheca made of stone with glass case, an unicum in the archeology of the death in the North-East part of the Iberian Peninsula. The paper is completed with a paleoanthropological and geological characterization of some of the most important evidences presented (including two pieces of green “campán” and turkish marble.

  17. Capacidade de suporte de carga e umidade crítica de um Latossolo induzida por diferentes manejos

    Directory of Open Access Journals (Sweden)

    Cezar Francisco Araujo-Junior

    2011-02-01

    partir do excedente das amostras indeformadas, foram determinados distribuição granulométrica de partículas, C orgânico do solo (COS e teor de óxidos. Modelos de capacidade de suporte de carga (CSC do tipo σp = 10(a+bq entre pressão de preconsolidação e umidade volumétrica foram obtidos para verificar os possíveis efeitos dos diferentes sistemas de manejo de plantas invasoras na estrutura do solo. A tensão máxima aplicada pelo trator cafeeiro foi de 220 kPa para o pneu dianteiro 6-16 na pressão de inflação de 172 kPa. O menor valor de umidade crítica foi de 0,27 cm³ cm-3 para o LVdf sob o manejo SCAP na profundidade de 0-3 cm, e o maior valor, de 0,48 cm³ cm-3 para o solo sob o manejo HPRE na profundidade de 0-3 cm. O manejo de plantas invasoras utilizando GRAD e HPRE favorece a formação do encrostamento superficial e os incrementos dos valores de densidade do solo e capacidade de suporte de carga na profundidade de 0-3 cm. O solo sob MATA apresenta menor capacidade de suporte de cargas nas três profundidades estudadas, em relação ao solo cultivado com cafeeiros e submetido a diferentes manejos de plantas invasoras. Os diferentes manejos de plantas invasoras utilizadas no centro das entrelinhas da lavoura cafeeira não influenciaram a densidade do solo e o teor de C orgânico do Latossolo, na profundidade de 25-28 cm, em relação ao solo sob mata nativa.

  18. ExplorOcean H2O SOS: Help Heal the Ocean-Student Operated Solutions: Operation Climate Change

    Science.gov (United States)

    Weiss, N.; Wood, J. H.

    2016-12-01

    The ExplorOcean H2O SOS: Help Heal the Ocean—Student Operated Solutions: Operation Climate Change, teaches middle and high school students about ocean threats related to climate change through hands-on activities and learning experiences in the field. During each session (in-class or after-school as a club), students build an understanding about how climate change impacts our oceans using resources provided by ExplorOcean (hands-on activities, presentations, multi-media). Through a student leadership model, students present lessons to each other, interweaving a deep learning of science, 21st century technology, communication skills, and leadership. After participating in learning experiences and activities related to 6 key climate change concepts: 1) Introduction to climate change, 2) Increased sea temperatures, 3) Ocean acidification, 4) Sea level rise, 5) Feedback mechanisms, and 6) Innovative solutions. H2O SOS- Operation Climate change participants select one focus issue and use it to design a multi-pronged campaign to increase awareness about this issue in their local community. The campaign includes social media, an interactive activity, and a visual component. All participating clubs that meet participation and action goals earn a field trip to ExplorOcean where they dive deeper into their selected issue through hands-on activities, real-world investigations, and interviews or presentations with experts. In addition to self-selected opportunities to showcase their focus issue, teams will participate in one of several key events identified by ExplorOcean, including ExplorOcean's annual World Oceans Day Expo.

  19. The Arabidopsis thaliana mutant air1 implicates SOS3 in the regulation of anthocyanins under salt stress

    KAUST Repository

    Van Oosten, Michael James

    2013-08-08

    The accumulation of anthocyanins in plants exposed to salt stress has been largely documented. However, the functional link and regulatory components underlying the biosynthesis of these molecules during exposure to stress are largely unknown. In a screen of second site suppressors of the salt overly sensitive3-1 (sos3-1) mutant, we isolated the anthocyanin-impaired-response-1 (air1) mutant. air1 is unable to accumulate anthocyanins under salt stress, a key phenotype of sos3-1 under high NaCl levels (120 mM). The air1 mutant showed a defect in anthocyanin production in response to salt stress but not to other stresses such as high light, low phosphorous, high temperature or drought stress. This specificity indicated that air1 mutation did not affect anthocyanin biosynthesis but rather its regulation in response to salt stress. Analysis of this mutant revealed a T-DNA insertion at the first exon of an Arabidopsis thaliana gene encoding for a basic region-leucine zipper transcription factor. air1 mutants displayed higher survival rates compared to wild-type in oxidative stress conditions, and presented an altered expression of anthocyanin biosynthetic genes such as F3H, F3′H and LDOX in salt stress conditions. The results presented here indicate that AIR1 is involved in the regulation of various steps of the flavonoid and anthocyanin accumulation pathways and is itself regulated by the salt-stress response signalling machinery. The discovery and characterization of AIR1 opens avenues to dissect the connections between abiotic stress and accumulation of antioxidants in the form of flavonoids and anthocyanins. © 2013 Springer Science+Business Media Dordrecht.

  20. Construction of a ColD cda Promoter-Based SOS-Green Fluorescent Protein Whole-Cell Biosensor with Higher Sensitivity toward Genotoxic Compounds than Constructs Based on recA, umuDC, or sulA Promoters

    DEFF Research Database (Denmark)

    Norman, Anders; Hansen, Lars Hestbjerg; Sørensen, Søren Johannes

    2005-01-01

    Four different green fluorescent protein (GFP)-based whole-cell biosensors were created based on the DNA damage inducible SOS response of Escherichia coli in order to evaluate the sensitivity of individual SOS promoters toward genotoxic substances. Treatment with the known carcinogen N-methyl-N'-......Four different green fluorescent protein (GFP)-based whole-cell biosensors were created based on the DNA damage inducible SOS response of Escherichia coli in order to evaluate the sensitivity of individual SOS promoters toward genotoxic substances. Treatment with the known carcinogen N......-cell biosensor which is not only able to detect minute levels of genotoxins but, due to its use of the green fluorescent protein, also a reporter system which should be applicable in high-throughput screening assays as well as a wide variety of in situ detection studies....

  1. Arremessar por cima do ombro e a distância percorrida pelo implemento

    OpenAIRE

    Silva,Samuel Bento da; Vilela Junior,Guanis de Barros; Toloka,Rute Estanislava

    2009-01-01

    A habilidade de arremessar por cima do ombro tem sido bastante estudada, observando-se a velocidade do implemento, porém enquanto muitas tarefas têm como objetivo arremessar o mais longe possível, a distância percorrida pelo objeto ainda não tem sido investigada. O objetivo deste estudo foi verificar se existe relação entre o estágio de desenvolvimento motor nesta habilidade e a distância percorrida pelo implemento e observar diferenças entre os gêneros masculino e feminino; 50 crianças, com ...

  2. Vibrational frequency scaling factors for correlation consistent basis sets and the methods CC2 and MP2 and their spin-scaled SCS and SOS variants

    Energy Technology Data Exchange (ETDEWEB)

    Friese, Daniel H., E-mail: daniel.h.friese@uit.no [Centre for Theoretical and Computational Chemistry CTCC, Department of Chemistry, University of Tromsø, N-9037 Tromsø (Norway); Törk, Lisa; Hättig, Christof, E-mail: christof.haettig@rub.de [Lehrstuhl für Theoretische Chemie, Ruhr-Universität Bochum, D-44801 Bochum (Germany)

    2014-11-21

    We present scaling factors for vibrational frequencies calculated within the harmonic approximation and the correlated wave-function methods coupled cluster singles and doubles model (CC2) and Møller-Plesset perturbation theory (MP2) with and without a spin-component scaling (SCS or spin-opposite scaling (SOS)). Frequency scaling factors and the remaining deviations from the reference data are evaluated for several non-augmented basis sets of the cc-pVXZ family of generally contracted correlation-consistent basis sets as well as for the segmented contracted TZVPP basis. We find that the SCS and SOS variants of CC2 and MP2 lead to a slightly better accuracy for the scaled vibrational frequencies. The determined frequency scaling factors can also be used for vibrational frequencies calculated for excited states through response theory with CC2 and the algebraic diagrammatic construction through second order and their spin-component scaled variants.

  3. Design and implementation of a radiation hardened silicon on sapphire (SOS) embedded signal conditioning unit controller (SCUC) for the RAPID instrument on the Cluster satellites

    International Nuclear Information System (INIS)

    Ersland, L.

    1992-07-01

    The Cluster mission consistens of four spacecrafts equipped with instruments capable of making comprehensive measurements of plasma particles and electromagnetic fields. The RAPID (Research with Adaptive Particle Imaging Detectors) spectrometer is one of many instruments on board the Cluster satellites. It is designed for fast analysis of energetic electrons and ions with a complete coverage of the unit sphere in phase space. This thesis describes the development and testing of an embedded controller for the Spectroscopic Camera for Electrons, Neutral and Ion Compositions (SCENIC), which is a part of the RAPID instrument. The design is implemented in two different CMOS circuit technologies, namely Actel's Field Programmable Gate Arrays and GEC Plessey's CMOS Silicon On Sapphire (SOS) gate array. The prototypes of the SOS gate array have been verified and characterized. This includes measurements of DC and AC parameters under different conditions, including total dose of gamma irradiation. 42 refs., 92 figs., 44 tabs

  4. Efeito da hiperprolactinemia induzida pela metoclopramida na córnea de camundongas Effects of metoclopramide-induced hyperprolactinemia on the murine corneal

    Directory of Open Access Journals (Sweden)

    Carina Verna

    2006-10-01

    Full Text Available OBJETIVO: Avaliar as alterações morfológicas promovidas pela hiperprolactinemia induzida pela metoclopramida na córnea de camundongas durante a fase de proestro e na gestação. MÉTODOS: Quarenta camundongas adultas foram divididas, aleatoriamente, em dois grupos, a saber: controle (CTR1 e metoclopramida (MET1. Após 50 dias metade dos animais de cada grupo foram sacrificados. O restante dos animais foi acasalado, constituindo dois grupos: controle prenhe (CTR2 e o metoclopramida prenhe (MET2, que foi sacrificado no 6º dia de gestação. Após decapitação dos animais coletou-se sangue para dosagens de estradiol, progesterona e prolactina, em seguida removidos os globos oculares para estudo histomorfométrico da córnea. RESULTADOS: As espessuras do epitélio, estroma, endotélio e a espessura total das córneas dos grupos experimentais: MET1 e MET2 mostraram-se mais espessados quando comparados com os grupos controles: CTR1 e CTR2, respectivamente. Houve redução dos níveis hormonais do estrogênio e da progesterona nos animais que receberam metoclopramida em comparação com os respectivos controles (CTR1: estradiol = 156,6±42,2 pg/ml; progesterona = 39,4±5,1 ng/ml; prolactina = 130,4±26,2 ng/ml; MET1: estradiol = 108,0±33,1 pg/ml; progesterona = 28,0±6,4 ng/ml; prolactina = 551,5± 23,3 ng/ml; CTR2: estradiol = 354,0±56,0 pg/ml; progesterona = 251,0± 56,0 ng/ml; prolactina = 423,2±28,1 ng/ml; MET2: estradiol = 293,0± 43,0 pg/ml; progesterona = 184,0±33,0 ng/ml; prolactina = 823,1±51,1 ng/ml. CONCLUSÃO: A hiperprolactinemia induzida pela metoclopramida produziu espessamento da córnea, sobretudo, em camundongas prenhes. Possivelmente este efeito está relacionado com a redução da produção hormonal de estrogênio e de progesterona.PURPOSE: To evaluate the morphological changes in murine cornea upon metoclopramide-induced hyperprolactinemia during the proestrous phase or pregnancy. METHODS: Forty adult mice were divided

  5. Citoesqueleto e mecanotransdução na fisiopatologia da lesão pulmonar induzida por ventilador Cytoskeleton and mechanotransduction in the pathophysiology of ventilator-induced lung injury

    Directory of Open Access Journals (Sweden)

    Leandro Utino Taniguchi

    2010-06-01

    Full Text Available A ventilação mecânica é uma terapia importante, mas pode resultar em complicações. Uma das mais relevantes é a lesão pulmonar induzida por ventilador. Devido à hiperdistensão alveolar, o pulmão inicia um processo inflamatório, com infiltrado neutrofílico, formação de membrana hialina, fibrogênese e prejuízo de troca gasosa. Nesse processo, a mecanotransdução da hiperdistensão celular é mediada através do citoesqueleto da célula e de suas interações com a matriz extracelular e com as células vizinhas, de modo que o estímulo mecânico da ventilação se traduz em sinalização bioquímica intracelular, desencadeando ativação endotelial, permeabilidade vascular pulmonar, quimiotaxia leucocitária, produção de citocinas e, possivelmente, lesão de órgãos à distância. Estudos clínicos demonstram essa relação entre distensão pulmonar e mortalidade em pacientes com lesão pulmonar induzida por ventilador. Entretanto, apesar de o citoesqueleto ter um papel fundamental na patogênese da lesão pulmonar induzida por ventilador, a literatura carece de estudos utilizando modelos in vivo sobre as alterações do citoesqueleto e de suas proteínas associadas durante esse processo patológico.Although mechanical ventilation is an important therapy, it can result in complications. One major complication is ventilator-induced lung injury, which is caused by alveolar hyperdistension, leading to an inflammatory process, with neutrophilic infiltration, hyaline membrane formation, fibrogenesis and impaired gas exchange. In this process, cellular mechanotransduction of the overstretching stimulus is mediated by means of the cytoskeleton and its cell-cell and cell-extracellular matrix interactions, in such a way that the mechanical stimulus of ventilation is translated into an intracellular biochemical signal, inducing endothelial activation, pulmonary vascular permeability, leukocyte chemotaxis, cytokine production and, possibly

  6. Peroxidase activity in roots of arracacha affected by pH and temperature = Atividade da peroxidase em raízes de batata-baroa afetada pelo pH e temperatura

    Directory of Open Access Journals (Sweden)

    Luciana Nunes Menolli

    2011-07-01

    Full Text Available In this paper, roots of arracacha (Arracacia xanthorrhyza Bancroft were stored at 5ºC to induce chilling injury symptoms and stress-related peroxidase activity. Later, peroxidase kinetic activity was determined in different pH and temperature conditions. For this, soluble crude extract was sequentially saturated with ammonium sulfate, obtaining a semi-purified enzyme solution used for the analysis. Activity of peroxidase induced by the chilling at 5oC was determined from pH 2.5 to 9.0 and at temperature ranging from 10 to80oC. The peroxidase had higher activity when the reaction occurred between pH 5.5 and 6.0 and at temperature of 30oC. Complete inactivation of the activity was observed in pH 2.5 after 60 minutes of pre-incubation or at 60oC for 10 minutes or alternatively at 70oCafter 5 minutes of pre-incubation. The enzyme is more susceptible to inactivation in acid than alkaline pHs or alternatively using heat treatment.Neste trabalho, raízes de batata-baroa (Arracacia xanthorrhiza Bancroft foram armazenadas a 5oC para induzir injúria por frio e expressar atividade da peroxidase de estresse. Posteriormente, a cinética de atividade foi determinada em diferentes condições depHs e temperatura. Para isto, extrato solúvel da raiz foi sequencialmente saturado com sulfato de amônio, obtendo-se uma preparação semi-purificada para a análise enzimática. Atividade peroxidativa induzida pela temperatura de armazenamento de 5oC foideterminada em pHs de 2,5 a 9,0 e a temperaturas de 10 a 80oC. A atividade da peroxidase foi maior quando a reação foi realizada nos pHs de 5,5 e 6,0 e temperatura de 30oC. A inativação completa da enzima ocorreu em pH de 2,5 após 60 min. de pré-incubação ou a60oC por 10 min., e alternativamente a 70oC após 5 min. de pré-incubação. A enzima foi mais susceptível à inativação em pH ácido do que alcalino, podendo também ser inativada pelo tratamento de calor.

  7. Empirical research on complex networks modeling of combat SoS based on data from real war-game, Part I: Statistical characteristics

    Science.gov (United States)

    Chen, Lei; Kou, Yingxin; Li, Zhanwu; Xu, An; Wu, Cheng

    2018-01-01

    We build a complex networks model of combat System-of-Systems (SoS) based on empirical data from a real war-game, this model is a combination of command & control (C2) subnetwork, sensors subnetwork, influencers subnetwork and logistical support subnetwork, each subnetwork has idiographic components and statistical characteristics. The C2 subnetwork is the core of whole combat SoS, it has a hierarchical structure with no modularity, of which robustness is strong enough to maintain normal operation after any two nodes is destroyed; the sensors subnetwork and influencers subnetwork are like sense organ and limbs of whole combat SoS, they are both flat modular networks of which degree distribution obey GEV distribution and power-law distribution respectively. The communication network is the combination of all subnetworks, it is an assortative Small-World network with core-periphery structure, the Intelligence & Communication Stations/Command Center integrated with C2 nodes in the first three level act as the hub nodes in communication network, and all the fourth-level C2 nodes, sensors, influencers and logistical support nodes have communication capability, they act as the periphery nodes in communication network, its degree distribution obeys exponential distribution in the beginning, Gaussian distribution in the middle, and power-law distribution in the end, and its path length obeys GEV distribution. The betweenness centrality distribution, closeness centrality distribution and eigenvector centrality are also been analyzed to measure the vulnerability of nodes.

  8. The Walker A motif mutation recA4159 abolishes the SOS response and recombination in a recA730 mutant of Escherichia coli.

    Science.gov (United States)

    Šimatović, Ana; Mitrikeski, Petar T; Vlašić, Ignacija; Sopta, Mary; Brčić-Kostić, Krunoslav

    2016-01-01

    In bacteria, the RecA protein forms recombinogenic filaments required for the SOS response and DNA recombination. In order to form a recombinogenic filament, wild type RecA needs to bind ATP and to interact with mediator proteins. The RecA730 protein is a mutant version of RecA with superior catalytic abilities, allowing filament formation without the help of mediator proteins. The mechanism of RecA730 filament formation is not well understood, and the question remains as to whether the RecA730 protein requires ATP binding in order to become competent for filament formation. We examined two mutants, recA730,4159 (presumed to be defective for ATP binding) and recA730,2201 (defective for ATP hydrolysis), and show that they have different properties with respect to SOS induction, conjugational recombination and double-strand break repair. We show that ATP binding is essential for all RecA730 functions, while ATP hydrolysis is required only for double-strand break repair. Our results emphasize the similarity of the SOS response and conjugational recombination, neither of which requires ATP hydrolysis by RecA730. Copyright © 2016 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

  9. Molecular Interaction and Cellular Location of RecA and CheW Proteins in Salmonella enterica during SOS Response and Their Implication in Swarming.

    Science.gov (United States)

    Irazoki, Oihane; Aranda, Jesús; Zimmermann, Timo; Campoy, Susana; Barbé, Jordi

    2016-01-01

    In addition to its role in DNA damage repair and recombination, the RecA protein, through its interaction with CheW, is involved in swarming motility, a form of flagella-dependent movement across surfaces. In order to better understand how SOS response modulates swarming, in this work the location of RecA and CheW proteins within the swarming cells has been studied by using super-resolution microscopy. Further, and after in silico docking studies, the specific RecA and CheW regions associated with the RecA-CheW interaction have also been confirmed by site-directed mutagenesis and immunoprecipitation techniques. Our results point out that the CheW distribution changes, from the cell poles to foci distributed in a helical pattern along the cell axis when SOS response is activated or RecA protein is overexpressed. In this situation, the CheW presents the same subcellular location as that of RecA, pointing out that the previously described RecA storage structures may be modulators of swarming motility. Data reported herein not only confirmed that the RecA-CheW pair is essential for swarming motility but it is directly involved in the CheW distribution change associated to SOS response activation. A model explaining not only the mechanism by which DNA damage modulates swarming but also how both the lack and the excess of RecA protein impair this motility is proposed.

  10. Estimation for aerial detection effectiveness with cooperation efficiency factors of early-warning aircraft in early-warning detection SoS under BSC framework

    Science.gov (United States)

    Zhu, Feng; Hu, Xiaofeng; He, Xiaoyuan; Guo, Rui; Li, Kaiming; Yang, Lu

    2017-11-01

    In the military field, the performance evaluation of early-warning aircraft deployment or construction is always an important problem needing to be explored. As an effective approach of enterprise management and performance evaluation, Balanced Score Card (BSC) attracts more and more attentions and is studied more and more widely all over the world. It can also bring feasible ideas and technical approaches for studying the issue of the performance evaluation of the deployment or construction of early-warning aircraft which is the important component in early-warning detection system of systems (SoS). Therefore, the deep explored researches are carried out based on the previously research works. On the basis of the characteristics of space exploration and aerial detection effectiveness of early-warning detection SoS and the cardinal principle of BSC are analyzed simply, and the performance evaluation framework of the deployment or construction of early-warning aircraft is given, under this framework, aimed at the evaluation issue of aerial detection effectiveness of early-warning detection SoS with the cooperation efficiency factors of the early-warning aircraft and other land based radars, the evaluation indexes are further designed and the relative evaluation model is further established, especially the evaluation radar chart being also drawn to obtain the evaluation results from a direct sight angle. Finally, some practical computer simulations are launched to prove the validity and feasibility of the research thinking and technologic approaches which are proposed in the paper.

  11. The SOS and RpoS Regulons Contribute to Bacterial Cell Robustness to Genotoxic Stress by Synergistically Regulating DNA Polymerase Pol II.

    Science.gov (United States)

    Dapa, Tanja; Fleurier, Sébastien; Bredeche, Marie-Florence; Matic, Ivan

    2017-07-01

    Mitomycin C (MMC) is a genotoxic agent that induces DNA cross-links, DNA alkylation, and the production of reactive oxygen species (ROS). MMC induces the SOS response and RpoS regulons in Escherichia coli SOS-encoded functions are required for DNA repair, whereas the RpoS regulon is typically induced by metabolic stresses that slow growth. Thus, induction of the RpoS regulon by MMC may be coincidental, because DNA damage slows growth; alternatively, the RpoS regulon may be an adaptive response contributing to cell survival. In this study, we show that the RpoS regulon is primarily induced by MMC-induced ROS production. We also show that RpoS regulon induction is required for the survival of MMC-treated growing cells. The major contributor to RpoS-dependent resistance to MMC treatment is DNA polymerase Pol II, which is encoded by the polB gene belonging to the SOS regulon. The observation that polB gene expression is controlled by the two major stress response regulons that are required to maximize survival and fitness further emphasizes the key role of this DNA polymerase as an important factor in genome stability. Copyright © 2017 by the Genetics Society of America.

  12. Ciprofloxacin provokes SOS-dependent changes in respiration and membrane potential and causes alterations in the redox status of Escherichia coli.

    Science.gov (United States)

    Smirnova, Galina V; Tyulenev, Aleksey V; Muzyka, Nadezda G; Peters, Mikhail A; Oktyabrsky, Oleg N

    2017-01-01

    An in-depth understanding of the physiological response of bacteria to antibiotic-induced stress is needed for development of new approaches to combatting microbial infections. Fluoroquinolone ciprofloxacin causes phase alterations in Escherichia coli respiration and membrane potential that strongly depend on its concentration. Concentrations lower than the optimal bactericidal concentration (OBC) do not inhibit respiration during the first phase. A dose higher than the OBC provokes immediate SOS-independent inhibition of respiration and growth that can contribute to a decreased SOS response and lowered susceptibility to high concentrations of ciprofloxacin. Cells retain their metabolic activity, membrane potential and accelerated K + uptake and produce low levels of superoxide and H 2 O 2 during the first phase. The time before initiation of the second phase is inversely correlated with the ciprofloxacin concentration. The second phase is SOS-dependent and characterized by respiratory inhibition, membrane depolarization, K + and glutathione leakage and cessation of glucose consumption and may be considered as cell death. atpA, gshA and kefBkefC knockouts, which perturb fluxes of protons and K + , can modify the degree and duration of respiratory inhibition and potassium retention. Loss of K + efflux channels KefB and KefC enhances the susceptibility of E. coli to ciprofloxacin. Copyright © 2016 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

  13. Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming

    Directory of Open Access Journals (Sweden)

    Oihane Irazoki

    2016-10-01

    Full Text Available In addition to its role in DNA damage repair and recombination, the RecA protein, through its interaction with CheW, is involved in swarming motility, a form of flagella-dependent movement across surfaces. In order to better understand how SOS response modulates swarming, in this work the location of RecA and CheW proteins within the swarming cells has been studied by using super-resolution microscopy. Further, and after in silico docking studies, the specific RecA and CheW regions associated with the RecA-CheW interaction have also been confirmed by site-directed mutagenesis and immunoprecipitation techniques. Our results point out that the CheW distribution changes, from the cell poles to foci distributed in a helical pattern along the cell axis when SOS response is activated or RecA protein is overexpressed. In this situation, the CheW presents the same subcellular location as that of RecA, pointing out that the previously described RecA storage structures may be modulators of swarming motility. Data reported herein not only confirmed that the RecA-CheW pair is essential for swarming motility but it is directly involved in the CheW distribution change associated to SOS response activation. A model explaining not only the mechanism by which DNA damage modulates swarming but also how both the lack and the excess of RecA protein impair this motility is proposed.

  14. Quantitative Proteomic Analysis of Staphylococcus aureus Treated With Punicalagin, a Natural Antibiotic From Pomegranate That Disrupts Iron Homeostasis and Induces SOS.

    Science.gov (United States)

    Cooper, Bret; Islam, Nazrul; Xu, Yunfeng; Beard, Hunter S; Garrett, Wesley M; Gu, Ganyu; Nou, Xiangwu

    2018-05-01

    Staphylococcus aureus, a bacterial, food-borne pathogen of humans, can contaminate raw fruits and vegetables. While physical and chemical methods are available to control S. aureus, scientists are searching for inhibitory phytochemicals from plants. One promising compound from pomegranate is punicalagin, a natural antibiotic. To get a broader understanding of the inhibitory effect of punicalagin on S. aureus growth, high-throughput mass spectrometry and quantitative isobaric labeling was used to investigate the proteome of S. aureus after exposure to a sublethal dose of punicalagin. Nearly half of the proteins encoded by the small genome were interrogated, and nearly half of those exhibited significant changes in accumulation. Punicalagin treatment altered the accumulation of proteins and enzymes needed for iron acquisition, and it altered amounts of enzymes for glycolysis, citric acid cycling, protein biosynthesis, and purine and pyrimidine biosynthesis. Punicalagin treatment also induced an SOS cellular response to damaged DNA. Transcriptional comparison of marker genes shows that the punicalagin-induced iron starvation and SOS responses resembles those produced by EDTA and ciprofloxacin. These results show that punicalagin adversely alters bacterial growth by disrupting iron homeostasis and that it induces SOS, possibly through DNA biosynthesis inhibition. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Dominant negative umuD mutations decreasing RecA-mediated cleavage suggest roles for intact UmuD in modulation of SOS mutagenesis

    International Nuclear Information System (INIS)

    Battista, J.R.; Ohta, Toshihiro; Nohmi, Takehiko; Sun, W.; Walker, G.C.

    1990-01-01

    The products of the SOS-regulated umuDC operon are required for most UV and chemical mutagenesis in Escherichia coli. The UmuD protein shares homology with a family of proteins that includes LexA and several bacteriophage repressors. UmuD is posttranslationally activated for its role n mutagenesis by a RecA-mediated proteolytic cleavage that yields UmuD'. A set of missense mutants of umuD was isolated and shown to encode mutant UmuD proteins that are deficient in RecA-mediated cleavage in vivo. Most of these mutations are dominant to umuD + with respect to UV mutagenesis yet do not interfere with SOS induction. Although both UmuD and UmuD' form homodimers, the authors provide evidence that they preferentially form heterodimers. The relationship of UmuD to LexA, λ repressor, and other members of the family of proteins is discussed and possible roles intact UmuD in modulating SOS mutagenesis are discussed

  16. Sequence analysis of the Ras-MAPK pathway genes SOS1, EGFR & GRB2 in silver foxes (Vulpes vulpes): candidate genes for hereditary hyperplastic gingivitis.

    Science.gov (United States)

    Clark, Jo-Anna B J; Tully, Sara J; Dawn Marshall, H

    2014-12-01

    Hereditary hyperplastic gingivitis (HHG) is an autosomal recessive disease that presents with progressive gingival proliferation in farmed silver foxes. Hereditary gingival fibromatosis (HGF) is an analogous condition in humans that is genetically heterogeneous with several known autosomal dominant loci. For one locus the causative mutation is in the Son of sevenless homologue 1 (SOS1) gene. For the remaining loci, the molecular mechanisms are unknown but Ras pathway involvement is suspected. Here we compare sequences for the SOS1 gene, and two adjacent genes in the Ras pathway, growth receptor bound protein 2 (GRB2) and epidermal growth factor receptor (EGFR), between HHG-affected and unaffected foxes. We conclude that the known HGF causative mutation does not cause HHG in foxes, nor do the coding regions or intron-exon boundaries of these three genes contain any candidate mutations for fox gum disease. Patterns of molecular evolution among foxes and other mammals reflect high conservation and strong functional constraints for SOS1 and GRB2 but reveal a lineage-specific pattern of variability in EGFR consistent with mutational rate differences, relaxed functional constraints, and possibly positive selection.

  17. SOS response in bacteria: Inhibitory activity of lichen secondary metabolites against Escherichia coli RecA protein.

    Science.gov (United States)

    Bellio, Pierangelo; Di Pietro, Letizia; Mancini, Alisia; Piovano, Marisa; Nicoletti, Marcello; Brisdelli, Fabrizia; Tondi, Donatella; Cendron, Laura; Franceschini, Nicola; Amicosante, Gianfranco; Perilli, Mariagrazia; Celenza, Giuseppe

    2017-06-15

    RecA is a bacterial multifunctional protein essential to genetic recombination, error-prone replicative bypass of DNA damages and regulation of SOS response. The activation of bacterial SOS response is directly related to the development of intrinsic and/or acquired resistance to antimicrobials. Although recent studies directed towards RecA inactivation via ATP binding inhibition described a variety of micromolar affinity ligands, inhibitors of the DNA binding site are still unknown. Twenty-seven secondary metabolites classified as anthraquinones, depsides, depsidones, dibenzofurans, diphenyl-butenolides, paraconic acids, pseudo-depsidones, triterpenes and xanthones, were investigated for their ability to inhibit RecA from Escherichia coli. They were isolated in various Chilean regions from 14 families and 19 genera of lichens. The ATP hydrolytic activity of RecA was quantified detecting the generation of free phosphate in solution. The percentage of inhibition was calculated fixing at 100µM the concentration of the compounds. Deeper investigations were reserved to those compounds showing an inhibition higher than 80%. To clarify the mechanism of inhibition, the semi-log plot of the percentage of inhibition vs. ATP and vs. ssDNA, was evaluated. Only nine compounds showed a percentage of RecA inhibition higher than 80% (divaricatic, perlatolic, alpha-collatolic, lobaric, lichesterinic, protolichesterinic, epiphorellic acids, sphaerophorin and tumidulin). The half-inhibitory concentrations (IC 50 ) calculated for these compounds were ranging from 14.2µM for protolichesterinic acid to 42.6µM for sphaerophorin. Investigations on the mechanism of inhibition showed that all compounds behaved as uncompetitive inhibitors for ATP binding site, with the exception of epiphorellic acid which clearly acted as non-competitive inhibitor of the ATP site. Further investigations demonstrated that epiphorellic acid competitively binds the ssDNA binding site. Kinetic data were

  18. Os encantos da Veneza Americana e da propaganda pelo cinema: os filmes financiados pelo governo Sergio Loreto em Pernambuco (1922-1926

    Directory of Open Access Journals (Sweden)

    Luciana Corrêa de Araújo

    2013-06-01

    Full Text Available Este artigo aborda a produção cinematográfica financiada pelo governo Sergio Loreto em Pernambuco (1922-1926. Por meio de textos históricos, pesquisa em periódicos e análise fílmica, pretendemos destacar a relevância do estímulo oficial ao incipiente meio cinematográfico local e analisar como se constroem, nos filmes, as relações entre estratégias de propaganda e procedimentos estéticos e narrativos.

  19. Inspeção visual do colo uterino após aplicação de ácido acético no rastreamento das neoplasias intra-epiteliais e lesões induzidas por HPV Cervical visual inspection after application of acetic acid in screening intraepithelial neoplasia and HPV-induced lesions

    Directory of Open Access Journals (Sweden)

    Maria Rachel Aguiar Cordeiro

    2005-02-01

    Full Text Available OBJETIVO: estimar a validade da inspeção visual após aplicação de ácido acético (IVA no rastreamento das neoplasias intra-epiteliais cervicais (NIC e lesões induzidas por HPV, comparando seu desempenho com o da colpocitologia e da colposcopia. MÉTODOS: estudo de validação de teste diagnóstico realizado em 893 mulheres de 18 a 65 anos, rastreadas simultaneamente com colpocitologia, IVA e colposcopia, em unidade de saúde pública de Recife, PE. A IVA foi realizada por embrocação do colo com ácido acético a 5% e observação a olho nu, com auxílio de foco clínico comum. Considerou-se como positividade o achado de qualquer lesão aceto-branca no colo. O padrão-ouro foi o histopatológico de biópsia cervical, realizado sempre que qualquer um dos três testes resultasse anormal. Foram estimados e comparados os indicadores de validade de cada teste, com os respectivos intervalos de confiança a 95%. A concordância entre os resultados dos testes foi avaliada pelo coeficiente kappa (k. RESULTADOS: das 303 mulheres biopsiadas, o estudo histopatológico foi anormal em 24. Deste total, a IVA foi positiva em 22, conferindo-lhe sensibilidade estimada de 91,7%, especificidade de 68,9%, valor preditivo positivo de 7,5% e valor preditivo negativo de 99,7%. Comparando-se os intervalos de confiança a 95%, a IVA mostrou maior sensibilidade que a colpocitologia, mas com menores especificidade e valor preditivo positivo. Houve fraca concordância entre os resultados da IVA e da colpocitologia (k=0,02 e excelente concordância com os da colposcopia (k=0,93. CONCLUSÃO: a IVA foi muito mais sensível que a colpocitologia no rastreamento das NIC e lesões HPV-induzidas e teve o mesmo desempenho da colposcopia. Sua baixa especificidade foi responsável por um elevado número de resultados falso-positivos.PURPOSE: to estimate the validity of visual inspection of cervical intraepithelial neoplasia (CIN and HPV-induced lesion screening, after acetic

  20. Análise biomecânica comparativa entre os sistemas plate-nail e plate-rod em fraturas induzidas em fêmures de caninos: estudo ex-vivo

    OpenAIRE

    Mesquita, Luciane dos Reis

    2013-01-01

    O objetivo deste estudo foi comparar as propriedades mecânicas da associação da haste intramedular bloqueada e placa óssea (plate-nail) e da associação do pino intramedular e placa óssea (plate-rod), utilizadas em fraturas experimentalmente induzidas em fêmures de caninos. Vinte pares de fêmures de cães foram selecionados para receber os sistemas plate-nail e plate-rod. Em cada fêmur, uma ostectomia no terço médio da diáfise foi realizada e inserido o sistema selecionado. As construções foram...

  1. Avaliação histomorfométrica da movimentação dentária induzida em ratos com força contínua, contínua interrompida e intermitente

    OpenAIRE

    Tondelli, Pedro Marcelo [UNESP

    2011-01-01

    Introdução: O presente trabalho apresenta uma metodologia para a movimentação dentária induzida (MDI) e avalia a quantidade de movimentação dentária, as condições ósseas, radiculares e periodontais, após o emprego de força contínua (FC), contínua interrompida (FCI) e intermitente (FI). Material e Métodos: Utilizou-se 54 ratos da linhagem Wistar, em grupos de FC, FCI e FI, nos períodos de 5, 7 e 9 dias. Inicialmente foram realizadas extrações dos incisivos superiores direitos de cada animal, e...

  2. Effect of rifampicin and gentamicin on Shiga toxin 2 expression level and the SOS response in Escherichia coli O104:H4.

    Science.gov (United States)

    Fadlallah, Sukayna M; Rahal, Elias A; Sabra, Ahmad; Kissoyan, Kohar A B; Matar, Ghassan M

    2015-01-01

    A novel pathotype, Shiga toxin-producing Escherichia coli O104:H4, was the cause of a severe outbreak that affected European countries, mainly Germany, in 2011. The effect of different regimens of rifampicin and gentamicin were evaluated to determine possible treatment modes for the novel strain, and to evaluate the SOS response and its effect on toxin release. Pulsed-field gel electrophoresis (PFGE) was performed on the novel E. coli O104:H4 pathotype and two pre-outbreak E. coli O104:H4 CDC strains. Transcript levels of the stx2 and recA gene (SOS response inducer) were evaluated using quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) in the novel E. coli O104:H4 samples subjected to different regimens of rifampicin and gentamicin. Consequently, reverse passive latex agglutination (RPLA) was used to determine the Stx2 titers in these samples. Western blot was performed to determine the LexA levels (SOS response repressor) in E. coli O104:H4. The efficacy of treatment with antimicrobial agents was assessed in BALB/c mice. The outbreak and pre-outbreak strains are closely related as shown by PFGE, which demonstrated slight genomic differences between the three strains. The transcription level of the stx2 gene in the new pathotype was 1.41- and 1.75-fold that of the 2009 EL-2050 and 2009 EL-2071 pre-outbreak strains, respectively. Moreover, the transcription level of the stx2 gene in the new pathotype was substantially decreased as a result of treatment with the different concentrations of the antimicrobial agents, but was enhanced when the antibiotics were administered at two subinhibitory levels. RPLA data were in accordance with the qRT-PCR results. E. coli O104:H4 exposed to gentamicin at both sub-minimum inhibitory concentration (MIC) levels led to high transcription levels of the recA gene and lack of expression of the LexA protein, implying that the SOS response was activated. Rifampicin at both sub-MIC levels resulted in low

  3. A Syndemic Analysis of Alcohol Use and Sexual Risk Behavior Among Tourism Employees in Sosúa, Dominican Republic

    Science.gov (United States)

    Padilla, Mark B.; Guilamo-Ramos, Vincent; Godbole, Ramona

    2012-01-01

    The Dominican Republic has high rates of HIV infection and alcohol consumption. Unfortunately, little research has been focused on the broader sources of the synergy between these two health outcomes. We draw on syndemic theory to argue that alcohol consumption and sexual risk behavior are best analyzed within the context of culture and economy in Caribbean tourism spaces, which produce a synergy between apparently independent outcomes. We sampled 32 men and women working in the tourism industry at alcohol-serving establishments in Sosúa, Dominican Republic. Interviewees described alcohol consumption as an implicit requirement of tourism work, tourism industry business practices that foster alcohol consumption, and an intertwining relationship between alcohol and sexual commerce. The need to establish relationships with tourists, combined with the overconsumption of alcohol, contributed to a perceived loss of sexual control, which participants felt could impede condom use. Interventions should incorporate knowledge of the social context of tourism areas to mitigate the contextual factors that contribute to HIV infection and alcohol consumption among locals. PMID:21859907

  4. A syndemic analysis of alcohol use and sexual risk behavior among tourism employees in Sosúa, Dominican Republic.

    Science.gov (United States)

    Padilla, Mark B; Guilamo-Ramos, Vincent; Godbole, Ramona

    2012-01-01

    The Dominican Republic has high rates of HIV infection and alcohol consumption. Unfortunately, little research has been focused on the broader sources of the synergy between these two health outcomes. We draw on syndemic theory to argue that alcohol consumption and sexual risk behavior are best analyzed within the context of culture and economy in Caribbean tourism spaces, which produce a synergy between apparently independent outcomes. We sampled 32 men and women working in the tourism industry at alcohol-serving establishments in Sosúa, Dominican Republic. Interviewees described alcohol consumption as an implicit requirement of tourism work, tourism industry business practices that foster alcohol consumption, and an intertwining relationship between alcohol and sexual commerce. The need to establish relationships with tourists, combined with the overconsumption of alcohol, contributed to a perceived loss of sexual control, which participants felt could impede condom use. Interventions should incorporate knowledge of the social context of tourism areas to mitigate the contextual factors that contribute to HIV infection and alcohol consumption among locals.

  5. BIÓPSIA PULMONAR EM BEZERROS COM BRONCOPNEUMONIA INDUZIDA PELA Mannheimia haemolytica PULMONAR BIOPSY IN CALVES WITH BRONCHOPNEUMONIA INDUCED BY Mannheimia haemolytica

    Directory of Open Access Journals (Sweden)

    Daniel Pessoa Gomes da Silva

    2009-09-01

    Full Text Available Com o propósito de avaliar a segurança, a eficácia diagnóstica da biópsia pulmonar e comparar a possível ocorrência de complicações decorrentes da técnica, entre bezerros sadios e com broncopneumonia induzida, utilizaram-se dez bezerros (G1 sadios e vinte bezerros portadores de broncopneumonia, divididos em quatro grupos de cinco bezerros (G2 a G5, os quais foram biopsiados 12, 24, 48 e 72 horas após a inoculação com Mannheimia haemolytica, respectivamente. A presença de crepitação grossa, som submaciço à percussão e as alterações radiográficas auxiliaram no diagnóstico da broncopneumonia e localizaram a área pulmonar a ser biopsiada nos grupos G2 a G5. As alterações microscópicas, visualizadas nos animais do grupo G2 a G5, foram as de broncopneumonia fibrinopurulenta. Nos bezerros do grupo G1 as alterações relacionadas à técnica foram: tosse, epistaxe, dispneia mista, taquipneia e taquicardia. Cinco (25% bezerros com broncopneumonia desenvolveram hemotórax após a biópsia e as alterações relacionadas à técnica foram: taquipneia, taquicardia, tosse, dispneia mista, apatia, mucosas pálidas e decúbito. Conclui-se que a biópsia pulmonar permite o diagnóstico de broncopneumonia em bezerros, contudo as complicações decorrentes da técnica limitam o seu uso apenas nas situações em que os métodos convencionais não tenham possibilitado o diagnóstico.

    PALAVRAS-CHAVES: Bezerros, biópsia pulmonar, broncopneumonia, Mannheimia haemolytica.

    The purpose of this study was to evaluate the safety and diagnostic efficacy of lung biopsy and to compare the possible occurrence of complications due to this technique in healthy calves and in calves with bronchopneumonia. Thirty Holstein calves were divided into a group of ten healthy animals (G1 and into four experimental groups (G2 to G5 of five calves each

  6. Estimulação elétrica neuromuscular de média freqüência (russa em cães com atrofia muscular induzida Medium frequency neuromuscular electrical stimulation (russian in dogs with induced muscle atrophy

    Directory of Open Access Journals (Sweden)

    Charles Pelizzari

    2008-06-01

    Full Text Available A estimulação elétrica neuromuscular (EENM de média freqüência (Russa ou de Kotz pode ser empregada para a recuperação de massa muscular em animais apresentando atrofia muscular por desuso. Assim, o objetivo deste trabalho foi empregar a EENM de média freqüência no quadríceps femoral de cães com atrofia muscular induzida, avaliando-se a ocorrência de ganho de massa. Foram utilizados oito cães em dois grupos denominados de GI ou controle e de GII ou tratado. Para a indução da atrofia muscular, a articulação fêmoro-tíbio-patelar esquerda foi imobilizada por 30 dias. Após 48 horas da remoção, foi realizada a EENM nos cães do grupo II, três vezes por semana, com intervalo de 48 horas cada sessão, pelo período de 60 dias. Foram avaliadas a mensuração da perimetria da coxa, da goniometria do joelho, as enzimas creatina-quinase (CK e morfometria das fibras musculares em cortes transversais do músculo vasto lateral, colhido mediante a biópsia muscular. A EENM foi empregada no músculo quadríceps femoral numa freqüência de 2.500Hz, largura de pulso de 50% e relação de tempo on/off de 1:2. Não houve diferença significativa quanto aos valores de perimetria da coxa e a atividade da enzima CK entre os grupos I e II. Na goniometria, houve diminuição significativa (PThe medium frequency neuromuscular electrical stimulation (NMES (Russa or Kotz is designed for recuperation of muscle mass in dogs with muscular atrophy in disuse. This study aims to utilize medium frequency NMES on the femoral quadriceps of dogs with induced muscular atrophy and evaluate the occurrence of gain in mass. Eight dogs in two groups denominated GI, or control, and GII, or treated were used. For the induction of muscular atrophy, the left femoral-tibial-patellar joint was immobilized for 30 days. NMES treatment began 48 hours after the removal of the immobilization device on dogs from group II and was carried out three times per week, with an

  7. Comparação entre a lidocaína e a acupuntura no tratamento da taquicardia ventricular induzida com dopamina em equinos anestesiados com halotano Comparative study between lidocaine and acupunture in the treatment of ventricular tachycardia induced by dopamine in horses anesthetized with halothane

    Directory of Open Access Journals (Sweden)

    J.J. Cárdenas

    2009-08-01

    Full Text Available Os efeitos da lidocaina e da acupuntura nos pontos bilaterais associados ao pericárdio 6 (Pc6-Neiguan e ao coração 7 (C7-Shenmen, no tratamento da taquicardia ventricular (TV induzida por dopamina em equinos anestesiados com halotano, foram avaliados e comparados. Seis equinos, distribuídos em três grupos: grupo-controle (GC, grupo tratado com acupuntura (GA e grupo tratado com lidocaína (GL, foram anestesiados três vezes cada, com intervalo de uma semana entre cada anestesia. Avaliaram-se os parâmetros cardiovasculares (frequência cardíaca, pressão arterial e eletrocardiografia, os respiratórios (frequência respiratória, capnografía, saturação de hemoglobina e hemogasometria e o escore de recuperação. A dose arritmogênica da dopamina (DAD foi determinada a partir da infusão de 70µg/kg/min IV durante 10 minutos, sem interrupção, preenchendo o critério arritmogênico: quatro ou mais complexos ventriculares prematuros seguidos, com duração de pelo menos 15 segundos ou TV sustentada. O tempo médio de aparecimento da DAD ou da TV foi de 6,05±0,45 minutos nos animais não tratados, e a TV se reverteu espontaneamente aos 2,7±0,2 minutos. O grupo tratado com acupuntura reverteu a TV no tempo médio de 1,8±0,2 (PThe effects of lidocaine and acupuncture in the associated bilateral points, i.e. pericardium 6 (Pc 6- Neiguan and heart 7 (H7 - Shenmen, on the ventricular tachycardia (VT induced by dopamine were evaluated in horses anesthetized with halothane. Six horses were distributed in three groups: control group (CG, acupuncture treated group (AG, and lidocaine treated group (LG. They were anesthetized three times each one using halothane with one week interval between each anesthesic procedure. Cardiovascular (heart rate, arterial pressure, and ECG and respiratory (respiratory rate, capnometry, hemoglobin saturation, and blood gas analysis parameters and recovery score were evaluated. The arrhythmogenic dose of dopamine

  8. Respuesta reproductiva de ovejas de pelo sincronizadas con progesterona o prostaglandinas

    OpenAIRE

    Arroyo-Ledezma, Jaime; De La Torre-Barrera, Jannette; Ávila-Serrano, N. Ysac

    2013-01-01

    La sincronización ajusta los estros a tiempos específicos y permite usar tecnologías reproductivas. El objetivo de este estudio fue evaluar la respuesta reproductiva de 30 ovejas de pelo sincronizadas con progesterona (P4) o prostaglandinas. Las ovejas de pelo eran 5/8 Pelibuey x 2/8 Black Belly x 1/8 Dorper, de 3.5 a 4.0 años de edad, con peso promedio de 37.8 ±3.0 kg; el último parto fue 8 meses previos al experimento. Los tratamientos fueron; 1) aplicación por 11 d de 300 mg de P4 impregna...

  9. EXPRESSÕES ORAIS POPULARES UTILIZADAS PELO POVO DO LAVRADO EM RORAIMA

    Directory of Open Access Journals (Sweden)

    Sebastião Pereira Nascimento

    2016-08-01

    Full Text Available São apresentadas 564 expressões orais populares utilizadas pelos habitantes do lavrado em Roraima. A maioria dos significados dos termos é originária da herança cultural trazida pelo povo nordestino radicado nos Campos do Rio Branco desde o século XIX. As línguas indígenas também têm influência nestas expressões orais locais, com destaque para as famílias linguísticas Karib e Aruak. Alguns termos têm influências culturais de outras regiões brasileiras e fragmentos de línguas estrangeiras.

  10. Ontogênese de caneluras em pedúnculo de flores de laranjeira doce infectados pelo vírus da tristeza dos citros estirpe "Capão Bonito"

    Directory of Open Access Journals (Sweden)

    Francisco André Ossamu Tanaka

    2009-12-01

    Full Text Available Um estudo da ontogênese das caneluras induzidas em ramos de laranjeiras doces suscetíveis por isolados severos do vírus da tristeza dos citros (Citrus tristeza vírus - CTV foi feito usando-se como modelo pedúnculos florais e de frutos. O menor calibre destes órgãos permite um melhor acompanhamento do processo. As observações foram feitas em laranjeira cv. Pêra infetada pelo isolado severo Capão Bonito do CTV. Cinco fases do processo de formação de caneluras puderam ser deduzidas pelas análises anatômicas. As primeiras alterações são representadas pelo aparecimento de células adensadas, hipertrofia e hiperplasia no parênquima e câmbio do floema e uma desorganização generalizada desta área. Segue-se uma atividade intensa do câmbio do floema adjacente e sua expansão em direção ao xilema. Esta invasão do xilema resulta na ruptura do anel do xilema pela massa celular do floema constituída de células recém formadas de parede celular delgada. Esta invasão do floema em direção ao xilema inicia um processo de degeneração dos vasos e parênquima do xilema. Finalmente há um colapso completo da região do xilema invadida, que é substituída pela massa do floema, resultando na canelura, notada ao se remover a casca.An ontogenetic study of the stem pitting was carried out, using as a model system the peduncle of flowers and fruits of sweet orange cv. Pêra infected by a very severe isolate, Capão Bonito, of Citrus tristeza virus (CTV. Anatomical and ultrastructural analysis revealed five phases of this process. It starts with the localized appearance of chromatic cells in the phloem parenchyma, hypertrophy of companion and cambial cells and overall disorganization of the phloem and deposition of a dense material. The next step is characterized by the invasion of the xylem by an abnormal mass of phloem parenchymal cells formed by the disorganized proliferation. These changes lead to the third phase, where xylem ring is

  11. Assessment of genotoxicity and cytotoxicity of "lixeira" (Curatella americanaL. λ using the prophage induction test (SOS inductest

    Directory of Open Access Journals (Sweden)

    Juliana Brandstetter Vilar

    2009-09-01

    Full Text Available Curatella americana L., commonly known as "lixeira" in Brazil, has been used in folk medicine to treat ulcers and inflammations. The purpose of the present work was to evaluate the cytotoxic and genotoxic potential of the ethanolic extract of C. americana stem bark using the prophage λ induction test (SOS inductest. To evaluate the cytotoxicity of this plant, after treatment with different concentrations of the extract, Escherichia coli WP2s(λ cultures were diluted in M9 buffer, inoculated into LB plates, and incubated for 24 h at 37 °C. To assess genotoxicity, the lysogenic strain E. coli WP2s(λ was treated with different concentrations of the extract. Then, the lysogenic strain was added to the indicator strain (RJF013, LB(1/2(malt/amp, seeded into plates with the matches, and incubated for 24 h at 37 °C. After this period, the total number of colonies and the number of plaques were counted to evaluate C. americana cytotoxicity and genotoxicity, respectively. Our results showed that although the extract of "lixeira" did not modify the survival of bacteria (p > 0.05, it caused a significant increase in prophage λ induction, especially at the higher concentrations (pCuratella americana L., comumente conhecida como "lixeira" no Brasil, é utilizada em medicina popular para tratamento de úlceras e inflamações. O presente trabalho teve como objetivo avaliar o potencial citotóxico e genotóxico do extrato etanólico das cascas de C. americana utilizando o Induteste SOS. Para avaliar a citotoxicidade da planta, depois de tratadas com diferentes concentrações do extrato, culturas de E. coli WP2s(λ foram diluνdas em tampão M9 e semeadas em placas LB. Para avaliar a genotoxicidade da planta, a cepa lisogênica WP2s(λ de E. coli foi tratada com diferentes concentrações do extrato. Em seguida, esta foi adicionada à cepa indicadora (RJF013 e ambas foram semeadas em placas em meio LB(1/2(malt(amp. Todas as culturas foram incubadas por 24

  12. Detailed studies on seed oil of Salicornia SOS-7 cultivated at the egyptian border of Red Sea

    Directory of Open Access Journals (Sweden)

    El-Mallah, M. Hassan

    1994-12-01

    Full Text Available Eight lipid patterns of Salicornia seed oil have been elucidated using HPLC as the main tool of analysis in conjunction with capillary GLC. The whole oil is rich in linoleic (66.5% with lower amount of linolenic acid (1.4%. Of the 22 triglycerides (TG determined, three major TG containing 3 and 2 linoleic acyls were detected by HPLC. The total tocopherols content (720 ppm was determined directly in the oil by HPLC and it was found that alpha-T (49.1% and gamma-T (48.2% are predominating.
    The whole sterol profile, as determined by GLC, includes cholesterol (1.0%, brassicasterol (1.4%, campesterol (2.4%, 5-stigmasterol (8.7%, beta-sitosterol (23.0%, spinasterol (17.0%, isofucosterol (0.8%, 7-stigmastenol (42.0% and 7-avenasterol (3.7%. Furthermore, sterols patterns of the free (nonacylated sterols (FS, acylated sterols (AS, free sterylglycosides (FSG and acylated sterylglycosides (ASG were determined by HPLC as their anthroylnitriles. FS and AS were determined as their steryl-9-anthroylnitriles (S-9-AN whereas FSG and ASG were analysed as their sterylglycosidyl-1-anthroylnitriles (SG-1-AN at nanogram level.
    Comparing Salicornia SOS-7 oil with traditional freshwater seed oils, Salicornia exhibits mostly a unique lipids constitution.

    Se han dilucidado ocho perfiles lipídicos de aceite de semilla de Salicornia usando HPLC como principal herramienta de análisis junto con la CGL capilar. El aceite completo es rico en linoleico (66.5% con bajo contenido en ácido linolénico (1.4%. De los 22 triglicéridos (TG determinados, los tres mayoritarios que contenían 3 y 2 cadenas de linoleico se detectaron por HPLC. El contenido en tocoferoles totales (720 ppm se determinó directamente en el aceite por HPLC y se encontró al alfa-T (49.1% y al gamma-T (48.2% como predominantes. El perfil de esteroles completo, determinado por CGL, incluyó colesterol (1.0%, brasicasterol (1.4%, campesterol (2.4%, 5-estigmasterol (8.7%, beta

  13. Radiation effects in a CMOS/SOS/Al-Gate D/A converter and on-chip diagnostic transistors

    International Nuclear Information System (INIS)

    Brucker, G.J.; Heagerty, W.

    1976-01-01

    This paper presents the results obtained from total dose and transient radiation tests on a CMOS/SOS/Al-Gate D/A converter and on-chip diagnostic transistors. Samples were irradiated by cobalt-60 gamma rays under worst-case conditions, and by 10-MeV electron pulses of 50-ns and 4.4-μs duration. Devices were fabricated with three different insulators; the two discussed here are standard wet oxide and a pyrogenic oxide. Test transistors on the D/A chips made it possible to diagnose the failure modes of the converter and to evaluate some special designs. These consisted of standard edge p- and n-channel transistors, edgeless units, edgeless tetrode transistors, and an edgeless type transmission gate with a diode clamp from substrate to gate. The total dose results indicate that the pyrogenic oxide increased the failure dose of the operational amplifier portion of the converter from 10 3 rads (Si) to 2 x 10 6 rads (Si); however, the sample and hold failed after exposure to a low level of 10 3 rads (Si). Test devices indicated this to be due to the radiation-induced leakage current of the transmission gate which discharges the sample and hold capacitor. The diode clamp decreased the threshold voltage shifts and the leakage currents. The edgeless devices improved the device performance because of a more abrupt turn-on. Narrow-pulse test data indicated that the edgeless units produced less photocurrent than the edge units by about a factor of three to four. Converter upset levels are less than or equal to 10 9 rads/s due to precision requirements which make a few millivolt transients untenable

  14. Surgical results from treating children with syndactyly through the collective effort system at "SOS Hand Recife" between 2005 and 2009

    Directory of Open Access Journals (Sweden)

    Mauri Cortez

    2014-08-01

    Full Text Available Objective:to evaluate the results from and parents' satisfaction with treatment for children with syndactyly who were operated at the "SOS Hand Recife" hospital between 2005 and 2009.Methods:data for assessing the results were gathered from the patients' medical files. The subjective scores, which were ascertained prospectively, were as follows: greater than or equal to 9, good result; between 6 and 8, fair result; less than 6, poor result. The results were analyzed statistically. This study was approved by the institution's ethics committee.Results:among the 35 cases, 21 (60% consisted of simple syndactyly and 14 (40% were complex; 22 (62.8% were boys and 13 (37.1% were girls. The complex cases were predominantly among males. The main complications were infection (11.4%, bleeding (11.4% and pain (8.6%. There were more complications in the complex cases (42.8% than in the simple cases (33.3%. The mean scores from the parents' subjective evaluations were as follows: 7.6 for esthetics (7.7 in simple cases and 7.3 in complex cases; 8.2 for function (8.6 in simple cases and 7.6 in complex cases; 8.3 for the parents' general satisfaction level (8.6 in simple cases and 8.0 in complex cases; and 85.7% of the parents would recommend the surgery to others while 14.5% would not. A strong association was observed between the specialist's objective assessment and the scores given by the parents (p < 0.05.Conclusion:the surgical results from treating syndactyly presented differences between the simple and complex types, even though the parents' esthetic evaluations and satisfaction were similar.

  15. Tolerância ao fenômeno alucinatório induzido pelo zolpidem: relato de caso Tolerancia al fenómeno alucinatorio inducido por el zolpidem: relato de caso Tolerance to zolpidem-induced hallucinations: case report

    Directory of Open Access Journals (Sweden)

    Saint-Clair Bahls

    2005-12-01

    Full Text Available INTRODUÇÃO: O hipnótico zolpidem é considerado efetivo e seguro, entretanto, existem na literatura alguns relatos de caso de fenômeno alucinatório associado ao seu uso. Relataremos mais um caso de alucinações induzidas pelo zolpidem em paciente jovem do sexo feminino. DISCUSSÃO: Este caso, segundo nosso conhecimento, é o primeiro descrito na literatura que sugere a possibilidade de ocorrer, em alguns pacientes, o desenvolvimento de tolerância a esse efeito alucinatório adverso raro, porém, importante. Apresentamos uma discussão sobre algumas hipóteses farmacocinéticas e farmacodinâmicas deste fenômeno. CONCLUSÕES: Alertamos para a necessidade de atenção ao fato da indução alucinatória pelo zolpidem e sua tolerância, assim como a importância de seu melhor entendimento.INTRODUCTION: El hipnótico zolpidem es considerado efectivo y seguro. Sin embargo, hay en la literatura algunos relatos de caso de fenómeno alucinatorio asociado a su uso. Relataremos un caso más sobre alucinaciones inducidas por zolpidem en una paciente joven de sexo femenino. DISCUSIÓN: Este caso, según nuestro conocimiento, es el primero descrito en la literatura que sugiere la posibilidad de que en algunos pacientes ocurra el desarrollo de tolerancia a este efecto alucinatorio, adverso y raro, sin embargo, importante. Presentamos una discusión sobre algunas hipótesis farmacocinéticas y farmacodinámicas de este fenómeno. CONCLUSION: Por la necesidad de atención, alertamos al hecho de la inducción alucinatoria relacionada al zolpidem y a su tolerancia, así como a la importancia de comprenderla mejor.BACKGROUND: Zolpidem is reported to be a safe and effective hypnotic agent. In the literature, there are some case reports of hallucinations after zolpidem administration. We report one more case in witch zolpidem lead to hallucinations in young adult female. DISCUSSION: This case, to our knowledge, is the first to suggest the possibility of

  16. Comparative cytotoxic and genotoxic potential of 13 drinking water disinfection by-products using a microplate-based cytotoxicity assay and a developed SOS/umu assay.

    Science.gov (United States)

    Zhang, Shao-Hui; Miao, Dong-Yue; Tan, Li; Liu, Ai-Lin; Lu, Wen-Qing

    2016-01-01

    The implications of disinfection by-products (DBPs) present in drinking water are of public health concern because of their potential mutagenic, carcinogenic and other toxic effects on humans. In this study, we selected 13 main DBPs found in drinking water to quantitatively analyse their cytotoxicity and genotoxicity using a microplate-based cytotoxicity assay and a developed SOS/umu assay in Salmonella typhimurium TA1535/pSK1002. With the developed SOS/umu test, eight DBPs: 3-chloro-4-(dichloromethyl)-5-hydroxy-2[5H]-fura3-chloro-4-(dichloromethyl)-5-hydroxy-2-[5H]-furanone (MX), dibromoacetonitrile (DBN), iodoacetic acid (IA), bromochloroacetonitrile (BCN), bromoacetic acid (BA), trichloroacetonitrile (TCN), dibromoacetic acid (DBA) and dichloroacetic acid (DCA) were significantly genotoxic to S. typhimurium. Three DBPs: chloroacetic acid (CA), trichloroacetic acid (TCA) and dichloroacetonitrile (DCN) were weakly genotoxic, whereas the remaining DBPs: chloroacetonitrile (CN) and chloral hydrate (CH) were negative. The rank order in decreasing genotoxicity was as follows: MX > DBN > IA > BCN > BA > TCN > DBA > DCA > CA, TCA, DCN > CN, CH. MX was approximately 370 000 times more genotoxic than DCA. In the microplate-based cytotoxicity assay, cytotoxic potencies of the 13 DBPs were compared and ranked in decreasing order as follows: MX > IA > DBN > BCN > BA > TCN > DCN > CA > DCA > DBA > CN > TCA > CH. MX was approximately 19 200 times more cytotoxic than CH. A statistically significant correlation was found between cytotoxicity and genotoxicity of the 13 DBPs in S. typhimurium. Results suggest that microplate-based cytotoxicity assay and the developed SOS/umu assay are feasible tools for analysing the cytotoxicity and genotoxicity of DBPs, particularly for comparing their toxic intensities quantitatively. © The Author 2015. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e

  17. Simulation of TunneLadder traveling-wave tube cold-test characteristics: Implementation of the three-dimensional, electromagnetic circuit analysis code micro-SOS

    Science.gov (United States)

    Kory, Carol L.; Wilson, Jeffrey D.

    1993-01-01

    The three-dimensional, electromagnetic circuit analysis code, Micro-SOS, can be used to reduce expensive time-consuming experimental 'cold-testing' of traveling-wave tube (TWT) circuits. The frequency-phase dispersion characteristics and beam interaction impedance of a TunneLadder traveling-wave tube slow-wave structure were simulated using the code. When reasonable dimensional adjustments are made, computer results agree closely with experimental data. Modifications to the circuit geometry that would make the TunneLadder TWT easier to fabricate for higher frequency operation are explored.

  18. The SOS Response Master Regulator LexA Regulates the Gene Transfer Agent of Rhodobacter capsulatus and Represses Transcription of the Signal Transduction Protein CckA.

    Science.gov (United States)

    Kuchinski, Kevin S; Brimacombe, Cedric A; Westbye, Alexander B; Ding, Hao; Beatty, J Thomas

    2016-02-01

    The gene transfer agent of Rhodobacter capsulatus (RcGTA) is a genetic exchange element that combines central aspects of bacteriophage-mediated transduction and natural transformation. RcGTA particles resemble a small double-stranded DNA bacteriophage, package random ∼4-kb fragments of the producing cell genome, and are released from a subpopulation (SOS response in many bacteria, as a regulator of RcGTA activity. Deletion of the lexA gene resulted in the abolition of detectable RcGTA production and an ∼10-fold reduction in recipient capability. A search for SOS box sequences in the R. capsulatus genome sequence identified a number of putative binding sites located 5' of typical SOS response coding sequences and also 5' of the RcGTA regulatory gene cckA, which encodes a hybrid histidine kinase homolog. Expression of cckA was increased >5-fold in the lexA mutant, and a lexA cckA double mutant was found to have the same phenotype as a ΔcckA single mutant in terms of RcGTA production. The data indicate that LexA is required for RcGTA production and maximal recipient capability and that the RcGTA-deficient phenotype of the lexA mutant is largely due to the overexpression of cckA. This work describes an unusual phenotype of a lexA mutant of the alphaproteobacterium Rhodobacter capsulatus in respect to the phage transduction-like genetic exchange carried out by the R. capsulatus gene transfer agent (RcGTA). Instead of the expected SOS response characteristic of prophage induction, this lexA mutation not only abolishes the production of RcGTA particles but also impairs the ability of cells to receive RcGTA-borne genes. The data show that, despite an apparent evolutionary relationship to lambdoid phages, the regulation of RcGTA gene expression differs radically. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  19. NKS/SOS-1 Seminar on Safety analysis. Report from a seminar held on 22-23 March 2000 Risø National Laboratory, Roskilde, DK

    DEFF Research Database (Denmark)

    The report describes presentations and discussions at a seminar held at Risø on March 22-23, 2000. The title of the seminar was NKS/SOS-1 – Safety Analysis. It dealt with issues of relevance for the safety analysis for the entire nuclear safety field (notably reactors and nuclear waste repositories......). Such issues were: objectives of safety analysis, risk criteria, decision analysis, expert judgement and risk communication. In addition, one talk dealt with criteria for chemical industries in Europe. The seminar clearly showed that the concept of risk is multi-dimensional, which makes clarity...

  20. Esteróide anabolizante inibe a angiogênese induzida pelo treinamento físico de natação em músculo sóleo de ratos normotensos Anabolic steroid impairs the angiogenesis induced by swimming training in soleus muscle of normotensive rats

    Directory of Open Access Journals (Sweden)

    Ursula Paula Reno Soci

    2009-09-01

    Full Text Available Os esteróides anabolizantes androgênicos (EAA são sintéticos de testosterona desenvolvidos para fins terapêuticos. São também utilizados por populações fisicamente ativas, que normalmente excedem nas doses, o que potencializa danos à saúde. Para estudar alguns dos efeitos de EAA sobre o sistema cardiovascular, ratos "Wistar" foram divididos em quatro grupos: sedentário controle (SC, sedentário anabolizado (SA, treinado controle (TC e treinado anabolizado (TA. Foram avaliados os efeitos da associação do uso de EAA (Decanoato de nandrolona - 5 mg/kg sc, 2x/sem e do treinamento físico de natação (TFN - 60 min/dia, 5x/sem, durante 10 sem sobre o débito cardíaco (DC e fluxo sanguíneo basal (DCbasal, Qbasal e após infusão do vasodilatador acetilcolina (DC Ach, Q Ach para observar a vasodilatação endotélio dependente (QAch, razão capilar/fibra (rc/f e expressão do fator de crescimento endotelial vascular (VEGF em músculo sóleo (predominância de fibras oxidativas. A testosterona plasmática aumentou nos grupos com uso de EAA e foi observada bradicardia de repouso como efeito do TFN. O DC foi menor para o Grupo TA, tanto na condição basal quanto sob infusão de Ach. O Qbasal não foi diferente entre os grupos no músculo estudado. O QAch foi maior no grupo TC, entretanto, no grupo TA este efeito benéfico do TFN foi prejudicado pela associação com o EAA. Aumento da rc/f e VEGF foi observado somente no grupo TC. Estes resultados sugerem que a associação do EAA ao TFN atenua a angiogênese e arteriogênese observadas como efeito do treinamento físico aeróbio e causa prejuízo ao fluxo sanguíneo muscular, o que poderia predispor o praticante de esportes e atividades físicas e usuário destas substâncias a problemas vasculares.Androgenic anabolic steroids (EAA are synthetic derivatives of testosterone, used in therapeutic dosages in medical practice and in high doses by physically active people that could be health damaging. To study the effects of EAA on the cardiovascular system, Wistar rats were randomized into Sedentary Control (SC, Sedentary Steroid (SA, Trained Control (TC and Trained Steroid (TA groups. We evaluated the effects of swimming training (60min/day, 5x/week during 10 week and AAS (nandrolone decanoate - 5 mg/kg sc, 2x/week on cardiac output, basal blood flow (Qb, DC basal and after injection of a vasodilator to observe the endothelium dependent vasodilatation (acetylcholine - Q Ach(Q Ach, DC Ach, capillary to fiber ratio (r c/f and vascular-endothelial growth factor expression (VEGF in soleus muscle (oxidative fibers. Serum testosterone increased in SA and TA. Exercise training significantly decreased resting heart rate. Qb was not different among groups, and QAch was higher in TC group, however in TA group this beneficial effect of swimming exercise training was lost by association with EAA. Rc/f and VEGF were higher only in TC group. These results suggest that swimming training associated with EAA inhibit angiogenesis and arteriogenesis observed as effects of aerobic training, and impairs the red skeletal muscle blood flow which predispose physically active AAS users to vascular diseases.

  1. Functional and morphological changes in the quadriceps muscle induced by eccentric training after ACL reconstruction Alterações funcionais e morfológicas do quadríceps induzidas pelo treinamento excêntrico após reconstrução do LCA

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    Jamilson S. Brasileiro

    2011-08-01

    Full Text Available OBJECTIVES: The purpose of this study was to investigate the contributions of functional and morphological factors in the recovery of the quadriceps muscle after anterior cruciate ligament (ACL reconstruction. METHODS: Nine subjects (31.3±5.8 years underwent eccentric exercise sessions twice a week for 12 weeks. Quadriceps muscle function was evaluated using an isokinetic dynamometer (isometric and eccentric peak torque and electromyography (RMS. Morphological changes were measured using magnetic resonance imaging. RESULTS: The initial evaluation showed a significant deficit in knee extensor torque in the involved limb and significant muscle atrophy along the length of the quadriceps. EMG activity was lower in all tested situations. Eccentric training significantly increased isokinetic torque (from 199±51 to 240±63, pOBJETIVOS: O propósito deste estudo foi avaliar as contribuições dos fatores funcionais e morfológicos na recuperação da força muscular do quadríceps femoral após reconstrução do Ligamento Cruzado Anterior (LCA. MÉTODOS: Nove indivíduos (31,3±5,8 anos foram treinados por meio de contrações excêntricas máximas, duas vezes por semana, durante 12 semanas. A função do quadríceps foi avaliada pela dinamometria isocinética (pico de torque isométrico e excêntrico e pela eletromiografia (EMG. As alterações morfológicas foram mensuradas por meio de ressonância magnética (RNM. Na avaliação inicial, observou-se significativo déficit no torque extensor do joelho do membro acometido, com hipotrofia muscular de todo o quadríceps e redução na atividade EMG, quando comparado ao membro não-acometido. RESULTADOS: O treinamento excêntrico produziu aumento no torque excêntrico a 30º/s (de 199±51 Nm para 240±63 Nm, p<0,05 e no volume muscular, sendo que maiores hipertrofias ocorreram na região proximal da coxa (de 169±27 para 189±25,8 cm², p<0,01. A atividade EMG do Vasto Medial (VM aumentou nas primeiras seis semanas de treinamento. O aumento no torque extensor demonstrou correlação positiva com o aumento no volume (r=0,81, p<0,01 e na atividade eletromiográfica (EMG (r=0,69, p<0,05 nas primeiras seis semanas. Após 12 semanas de treinamento, houve correlação apenas entre o aumento do torque e do volume (r=0,78, p<0,01. CONCLUSÕES: 1 O treinamento excêntrico mostrou-se como potente recurso tanto na recuperação dos fatores morfológicos como funcionais do músculo quadríceps; 2 A contribuição dos fatores neurais e morfológicos varia em função do período de treinamento.

  2. Efeito do treinamento físico em alterações induzidas pelo envelhecimento no músculo papilar do rato Efecto del entrenamiento físico en alteraciones inducidas por el envejecimiento en el músculo papilar de la rata Effect of exercise training on aging-induced changes in rat papillary muscle

    Directory of Open Access Journals (Sweden)

    Laura Beatriz Mesiano Maifrino

    2009-05-01

    Full Text Available FUNDAMENTO: Os efeitos do envelhecimento no músculo papilar têm sido amplamente demonstrados, mas não há dados disponíveis sobre os efeitos do exercício nas alterações relacionadas à idade. OBJETIVO: Analisar os efeitos do envelhecimento nas propriedades morfológicas e quantitativas do músculo papilar e investigar se um programa contínuo de exercícios moderados pode exercer um efeito protetor contra as conseqüências do envelhecimento. MÉTODOS: Microscopia eletrônica foi utilizada para estudar a densidade dos miócitos, capilares e tecido conectivo e área transversal dos miócitos do músculo papilar no ventrículo esquerdo de ratos Wistar de 6 e 13 meses, não-treinados e submetidos a exercícios. RESULTADOS: Como esperado, a densidade de volume dos miócitos diminui significantemente (pFUNDAMENTO: Los efectos del envejecimiento en el músculo papilar han sido demostrados de modo amplio, pero no hay datos disponibles sobre los efectos del ejercicio en las alteraciones relacionadas a la edad. OBJETIVO: Analizar los efectos del envejecimiento en las propiedades morfológicas y cuantitativas del músculo papilar e investigar si un programa continuo de ejercicios moderados puede ejercer un efecto protector contra las consecuencias del envejecimiento. MÉTODOS: Se utilizó microscopia electrónica para estudiar la densidad de los miocitos, capilares y tejido conectivo, así como el área transversal de los miocitos del músculo papilar en el ventrículo izquierdo de ratas Wistar de 6 y 13 meses, no entrenadas y sometidas a ejercicios. RESULTADOS: Como se esperaba, la densidad de volumen de los miocitos disminuye significantemente (pBACKGROUND: The effects of aging on papillary muscle have been widely demonstrated, but no data on the effects of exercise on the age-related changes are available. OBJECTIVE: To analyze the effects of aging on the morphological and quantitative properties of papillary muscle and investigate whether a long-term moderate exercise program would exert a protective effect against the effects of aging. METHODS: We used electron microscopy to study the density of myocytes, capillaries and connective tissue and the cross-sectional area of myocytes of the papillary muscle of the left ventricle of 6- and 13-month-old untrained and exercised Wistar rats. RESULTS: As expected, the volume density of myocytes declined significantly (p<0.05 with aging. The length density of myocardial capillaries also declined with aging, but not significantly. The interstitial volume fraction of the papillary muscle tissue increased significantly (P<0.05 with age. The number of myocyte profiles showed a reduction of 20% that was accompanied by myocyte hypertrophy in the aged rats (P<0.05. Animals submitted to a 60-minute daily session,, 5 days/wk at 1.8 km.h-1 of moderate running on a treadmill for 28 weeks showed a reversion of all the observed aging effects on papillary muscle. CONCLUSION: The present study supports the concept that long-term exercise training restrains the aging-related deleterious changes in the papillary muscle.

  3. The concept of ’Musa-pelo and the medicinal use of shrubby legumes (Fabaceae in Lesotho

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    A. Moteetee

    2007-08-01

    Full Text Available In the Kingdom of Lesotho, 20 plant species are commonly known as  'Musa-pelo. The term literally means ‘the one who brings back or tums around the heart’.  'Musa-pelo is traditionally used as a sedative and is given as a first aid treatment to bereaved people who are under severe psychological duress or stress. Of the 20 species known as  'Musa-pelo, 17 belong to nine genera of the Fabaceae  (Argyrolobium, Crotalaria, Indigofera, Lessertia, Lotononis, Melolobium, Sutherlandia, Tephrosia and  Trifolium. The three remaining species namely Cleome monophylla, Heliophila carnosa and  Cysticapnos pruinosa, belong to the families Capparaceae, Brassicaceae and Fumariaceae, respectively. In this paper, the concept of 'Musa-pelo in traditional medicine is explored.

  4. Perda auditiva induzida por ruído e hipertensão em condutores de ônibus Noise-induced hearing loss and high blood pressure among city bus drivers

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    Heleno Rodrigues Corrêa Filho

    2002-12-01

    Full Text Available OBJETIVO: Estimar as prevalências de perda auditiva induzida por ruído e hipertensão arterial em condutores de ônibus urbanos. MÉTODOS: Executou-se estudo transversal em amostra probabilística de 108 motoristas da cidade de Campinas, SP. Aplicou-se questionário sobre história profissional, jornadas de trabalho e repouso, e realizou-se exame físico e laboratorial incluindo medida da pressão arterial, audiometria tonal limiar, logoaudiometria e dados antropométricos, após a obtenção de consentimento. RESULTADOS: A prevalência de perda auditiva induzida por ruído foi de 32,7% do total examinado. Segundo a classificação de Merluzzi, nos 31 casos classificados em primeiro e segundo graus, observou-se que a freqüência audiométrica com perda auditiva mais acentuada foi a de 6 kHz (61,3%, seguida pela de 4 kHz (38,7%, sem diferenças significantes quanto à lateralidade. A prevalência de hipertensão arterial diastólica (PAD³90 mmHG; PAS³140 mmHG foi de 13,2% dos examinados. CONCLUSÕES: O risco de disacusia induzida por ruído foi maior para os motoristas com mais de seis anos de trabalho, após ajuste para a perda relacionada com a idade, com um odds ratio de 19,25 (1,59OBJECTIVE: To estimate the prevalence of occupational noise-induced hearing loss and arterial hypertension among city bus drivers. METHODS: A cross-sectional study was carried out on a probability random sample of 108 city bus drivers taken out of a total of 1,529 estimated professionals in the city of Campinas, Brazil, in 1991. Drivers were interviewed using questionnaires on job history, shift work and vacation schedules and underwent clinical and laboratory examinations including measures of blood pressure, pure-tone audiometry, speech audiometry, and anthropometric data analysis after an informed consent was obtained. RESULTS: The prevalence of noise-induced hearing loss was 32.7%. According to Merluzzi's classification, 91.2% (31 cases were classified as

  5. Peripheral Quantitative Computed Tomography (pQCT), Broad Band Ultrasound Attenuation (BUA) and Speed of Sound (SOS) in a population of normal females aged from 8 to 20 years

    International Nuclear Information System (INIS)

    Bagni, B.; Corazzari, T.; Bagni, I.; Garuti, F.; Franceschetto, A.; Casolo, A.; Pansini, F.

    2002-01-01

    Aim: To evaluate, in a population of young healthy females aged from 8 to 20 years the bone mass peak (or density), the normal ranges versus age and menarche-age using two method: pQCT (peripheral Quantitative Computed Tomography) and ultrasound absorptiometry. Material and Methods: From 1998 to 2000 selective measurement of Bone Mineral Density (BMD) of trabecular bone at the ultradistal radius using pQCT, BUA (Broad Band Attenuation) and SOS ( Speed Of Sound) was carried out on 426 healthy females (aged from 8 to 20 years) in north Italy. BMD were measured using a single photon miniaturized tomographic scanner in the ultradistal radius, SOS and BUA were measured at the same time, using a water bath device obtaining parametric bidimensional images of BUA and SOS. The population studied refers to normal females free of bone metabolism alteration, in pre and post-pubertal status. Results: A normal range of BMD, BUA and SOS versus age and menarche age were established. A linear correlation was found between BUA and BMD measured with pQCT. SOS does not show any correlation with BMD. The pre-puberty and the post-puberty groups show statistically significant differences between SOS, BUA and BMD. We found the peak bone density (measured with pQCT) in the trabecular bone at the ultradistal radius at 15 years of age (mean menarche age of 10 years). The same position of the peak was found for BUA, for SOS the situation is not well defined. The analytical fitting of the data highlights a polynomial correlation of BMD vs. age, SOS vs. age, BUA vs. age. Conclusions: It appears that the sexual growth influences the position of peak bone density. The results obtained show a statistically significant correlation between BUA and BMD versus age, the menarche-age and the period of exposure of bone tissue to the oestrogen. After all, pQCT and ultrasound are useful techniques to evaluate bone density and structure also in a growing population. The results of this study shows the

  6. Surto de malária induzida entre usuários de drogas injetáveis Outbreak of malaria among injectable-drug users

    Directory of Open Access Journals (Sweden)

    Luiz Carlos Barradas Barata

    1993-02-01

    Full Text Available Em julho de 1990, foi registrado na cidade de Bauru, Estado de São Paulo, Brasil, um surto de malária envolvendo usuários de cocaína injetável. Uma ampla investigação epidemiológica, conduzida de 19 de julho a 13 de setembro, revelou que pelo menos 119 pessoas estavam envolvidas no surto, uma vez que haviam compartilhado seringas e agulhas com um ou mais casos confirmados nos 3 meses anteriores à ocorrência. Cento e duas dessas pessoas foram localizadas e entrevistadas, e destas, 99 foram submetidas a exame de gota espessa e 91 a exames sorológicos para malária. Foram confirmados por exame hemoscópico 21 casos de malária por P. vivax, e 3 outros tiveram exame sorológico positivo para P. vivax. O controle da transmissão foi obtido fornecendo-se cloroquina aos envolvidos no surto, numa dose inicial de 10 comprimidos, seguida de doses supressivas semanais de 2 comprimidos até que fosse identificado o último comunicante. Amostras de soro coletadas na ocasião revelaram, ao lado da malária, uma alta prevalência de infecções pelo HIV (58% e pelo vírus da hepatite B (40%. Foram discutidas as dificuldades para o controle do surto e a possibilidade da malária vir a se tornar uma doença endêmica entre usuários de drogas injetáveis, no Estado de São Paulo.Five cases of malaria were detected among cocaine users by the local health service in Bauru, a city with a population of 260,000, located 324 km from S. Paulo, Brazil, during the first three weeks of July 1990. Autochthonous malaria had not occurred in Bauru since 1978, and all the five cases denied having recently traveled to endemic areas. An extensive epidemiologic survey conducted from July 19 to September 13 revealed that the 5 cases were in fact part of a malaria outbreak among endovenous drug users. Moreover, at least 114 other people, who had in the last three months shared syringes and needles with one or more proved cases, were also involved in the outbreak. One

  7. A diversidade de lagartos na Caatinga é mediada pelos térmitas?

    OpenAIRE

    Souza, Heitor Bruno de Araujo

    2012-01-01

    Ao tentar explicar a extraordinária taxa de coexistência de lagartos em desertos Australianos, Morton & James (1988) formularam uma hipótese em que a diversidade de térmitas promoveria uma diversificação dos lagartos. O presente estudo visa verificar se essa hipótese é válida para as taxocenoses de lagartos da Caatinga. Objetiva-se também verificar se os mecanismos de defesa dos térmitas influenciam a taxa com que esses são consumidos pelos lagartos e se esse padrão difere entre diferentes li...

  8. Cyclic AMP Regulates Bacterial Persistence through Repression of the Oxidative Stress Response and SOS-Dependent DNA Repair in Uropathogenic Escherichia coli.

    Science.gov (United States)

    Molina-Quiroz, Roberto C; Silva-Valenzuela, Cecilia; Brewster, Jennifer; Castro-Nallar, Eduardo; Levy, Stuart B; Camilli, Andrew

    2018-01-09

    Bacterial persistence is a transient, nonheritable physiological state that provides tolerance to bactericidal antibiotics. The stringent response, toxin-antitoxin modules, and stochastic processes, among other mechanisms, play roles in this phenomenon. How persistence is regulated is relatively ill defined. Here we show that cyclic AMP, a global regulator of carbon catabolism and other core processes, is a negative regulator of bacterial persistence in uropathogenic Escherichia coli , as measured by survival after exposure to a β-lactam antibiotic. This phenotype is regulated by a set of genes leading to an oxidative stress response and SOS-dependent DNA repair. Thus, persister cells tolerant to cell wall-acting antibiotics must cope with oxidative stress and DNA damage and these processes are regulated by cyclic AMP in uropathogenic E. coli IMPORTANCE Bacterial persister cells are important in relapsing infections in patients treated with antibiotics and also in the emergence of antibiotic resistance. Our results show that in uropathogenic E. coli , the second messenger cyclic AMP negatively regulates persister cell formation, since in its absence much more persister cells form that are tolerant to β-lactams antibiotics. We reveal the mechanism to be decreased levels of reactive oxygen species, specifically hydroxyl radicals, and SOS-dependent DNA repair. Our findings suggest that the oxidative stress response and DNA repair are relevant pathways to target in the design of persister-specific antibiotic compounds. Copyright © 2018 Molina-Quiroz et al.

  9. The success of opening single chronic total occlusion lesions to improve myocardialviabilitytrial (SOS-COMEDY): Study protocol of a prospective multicenter study.

    Science.gov (United States)

    Huang, Rongchong; Song, Xiantao; Zhang, Haishan; Tian, Wen; Huang, Zheng; Zhang, Xingwei; Yang, Junqing; Zhang, Dongfeng; Wu, Jian; Zhong, Lei; Ting, Henry H

    2018-04-01

    Success of opening single (SOS)-comedy is a prospective multicenter study to compare the improvement in the decrease of myocardial viability by percutaneous coronary intervention (PCI) with that by optimal medical therapy (OMT) alone in patients with chronic total occlusion (CTO) of a single coronary artery. The risks and the benefits of both options (PCI and OMT) were listed in a CTO decision aid (DA). Eligible participants detected by invasive coronary angiography (ICA) or coronary computed tomography angiography (CCTA) were divided into PCI or OMT groups according to patients' choice after shared-decision making process with DA. Participants will undergo positron emission tomography/computed tomography (PET/CT), cardiac magnetic resonance (CMR) and transthoracic echocardiography (TTE), and proceed to ICA and revascularization if possible. Blinded core laboratory interpretation will be performed for ICA, CCTA, PET/CT, CMR, and TTE. All participants will be followed up for 12 months. The primary endpoint is the improvement to the decrease of myocardial viability from baseline assessed with the use of PET/CT after 12-month follow-up. All of the patients are appropriately consented before enrolling in this study, which has been approved by the Ethics Committee. Results of SOS-COMEDY will be helpful to develop a strategy for single CTO patients.

  10. Una ciudad de los vascones en el yacimiento de Campo Real/Fillera (Sos del Rey Católico-Sangüesa

    Directory of Open Access Journals (Sweden)

    Andreu Pintado, Javier

    2008-12-01

    Full Text Available The following paper deals with the detailed and preliminary study of the archaeological site of Campo Real/Fillera (Sos del Rey Católico/Sangüesa, between today Zaragoza and Navarra provinces specially focusing in its Roman period. The paper exposes a review of the archeological and epigraphical material from the site, proposes an urban condition for it and raises some hypothesis on its identification with one of the cities that ancient sources tribued to Vascones.El presente trabajo aborda el estudio detallado y preliminar del yacimiento arqueológico de Campo Real/Fillera (Sos del Rey Católico/Sangüesa, en el límite entre las actuales provincias de Zaragoza y Navarra con especial atención a su etapa romana. Se procede a la revisión del material arqueológico y epigráfico procedente del lugar, se defiende la condición de enclave urbano del yacimiento y se plantea una hipótesis respecto de su identificación con las ciudades que las fuentes antiguas atribuyen a los Vascones.

  11. Translesion DNA synthesis and mutation induced in a plasmid with a single adduct of the environmental contaminant 3-nitrobenzanthrone in SOS-induced Escherichia coli

    International Nuclear Information System (INIS)

    Kawanishi, M.; Kanno, T.; Yagi, T.; Enya-Takamura, T.; Fuchs, R.P.

    2003-01-01

    Full text: 3-Nitrobenzanthrone (NBA) is a powerfully mutagenic nitrated aromatic hydrocarbon found in diesel exhaust and in airborne particulate matters. NBA forms an unusual DNA adduct in vitro that has a C-C bond between the C-8 position of deoxyguanosine and the C-2 position of NBA. We previously found that this adduct is also present in the human cells treated with NBA, and induces mutations in supF shuttle vector system. In this study, we analyzed translesion DNA synthesis (TLS) over a single adduct in lacZ' gene in a plasmid in uvrAmutS Escherichia coli. The result showed that the adduct blocked DNA replication and an observed TLS frequency was 5.4% in non-SOS-induced E. coli. All progenies after the TLS had no mutation. On the other hand, TLS increased to 11.3%, and 4.8% of them had mostly G to T mutations in SOS-induced E. coli. These results suggest that this unusual adduct would be one of causes of lung cancer that is increasing in the urban areas polluted with diesel exhaust. It must be interesting to reveal which DNA polymerase is involved in this TLS

  12. Photochemistry in Power Plant and Urban Plumes over Forested and Agricultural Regions during SOS (1990s) and SENEX (2013) field intensives (Invited)

    Science.gov (United States)

    Trainer, M.; Frost, G. J.; Kim, S.; Ryerson, T. B.; Pollack, I. B.; Roberts, J. M.; Veres, P. R.; Flocke, F. M.; Neuman, J. A.; Nowak, J. B.; Nenes, A.; Warneke, C.; Graus, M.; Gilman, J.; Lerner, B. M.; Kuster, W.; Atlas, E. L.; Hanisco, T. F.; Wolfe, G. M.; Keutsch, F. N.; Kaiser, J.; Lee, Y.; Brock, C. A.; Middlebrook, A. M.; Liao, J.; Welti, A.; Parrish, D. D.; Fehsenfeld, F. C.; De Gouw, J. A.

    2013-12-01

    Extensive forested regions of the southeastern United States show high emissions of biogenic reactive hydrocarbons such as isoprene, while emissions of these compounds are typically much lower from agricultural areas. The Southern Oxidant Study (SOS) field intensives during the 1990s contributed to an improved understanding of ozone (O3) formation resulting from nitrogen oxides (NOx) emitted from urban areas and power plants in the presence and absence of the biogenic hydrocarbons. Decreases in NOx emissions from power plants and urban areas have contributed to the widespread reduction of ambient O3 over the southeastern US during the past two decades. Measurements of Volatile Organic Compounds (VOCs), NOx, and their reaction products made at successive distances downwind of emission sources during the SOS (1999) and the Southeast Nexus (SENEX, 2013) campaigns reflect the modulation of the photochemical processing of biogenic VOCs by ambient NOx concentrations. The results constrain the ambient levels of HOx radicals as a function of NOx, and they reflect the mechanisms of the coupling between anthropogenic and biogenic emissions that form species such as ozone, formaldehyde, PeroxyAcetic Nitric anhydride (PAN), nitric acid, as well as, inorganic and organic aerosols.

  13. Conhecendo as estrategias de acao e interacao utilizadas pelos clientes para o enfrentamento da dialise peritoneal

    Directory of Open Access Journals (Sweden)

    Felipe Kaezer dos Santos

    2013-09-01

    Full Text Available Este artigo teve por objetivo apresentar as principais estratégias de ação e interação utilizadas pelos clientes portadores de doença renal crônica para o enfrentamento da diálise peritoneal. O referencial teórico utilizado foi o Interacionismo Simbólico, por ser um arcabouço que valoriza o significado da interação como determinante do comportamento humano. A Teoria Fundamentada nos Dados foi utilizada como referencial metodológico. Após a sua obtenção, realizada por meio de um roteiro de entrevista semi-estruturado, os dados foram gravados, transcritos, codificados e organizados para compor a teoria explicativa sobre o fenômeno estudado. Durante a análise foram identificados vários recursos utilizados pelos clientes, como a necessidade de obter informações sobre a diálise peritoneal, o refúgio na espiritualidade e a falsa ilusão da cura. Destaca-se também a importância da rede de apoio, em que o enfermeiro poderá atuar como facilitador do processo de enfrentamento, em prol da adaptação dos clientes à nova realidade.

  14. A Disputa pelo sentido: o jogo de poder na Comunicação Organizacional

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    Márcia Maria Garçon

    2013-12-01

    Full Text Available Este trabalho adotou como aporte teórico, os clássicos de Blumer (1980, Bakhtin (1995, Foucalt (1996 e Galbraith (1986 e os estudos contemporâneos da Comunicação Organizacional pautados no paradigma da produção de sentido (OLIVEIRA; PAULA, 2008 e da gestão sistêmica (YANAZE, 2011 com o objetivo de identificar as relações de poder e luta pelo sentido do projeto social Comunidade Educativa, promovido pela Fundação Bunge na E.E. Henrique Dumont Villares, na capital paulista. Foram realizadas entrevistas qualitativas, em profundidade, com os representantes da Fundação e da Escola e suas narrativas, classificadas em categorias léxico-semânticas de semelhança, complementaridade, diversidade e divergência, sendo posteriormente confrontadas, a fim de identificar o embate dos sentidos. Os resultados apontaram que os interesses particulares pautaram a luta pelo sentido e o poder, que a princípio esteve de posse da Fundação, migrou para o campo dos professores, sendo o significado construído entre estes sujeitos, o hegemônico nesta interação.

  15. “12 ANOS DE ESCRAVIDÃO” E A PAIXÃO PELO REAL

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    Luís Gustavo Finger de Oliveira

    2015-12-01

    Full Text Available Este artigo propõe uma reflexão sobre as narrativas audiovisuais contemporâneas marcadas pelo choque do real ou pela paixão pelo real. Em perspectivas semelhantes, os autores defendem a ideia de que, diante da experiência virtualizada e fragmentada atual, as produções audiovisuais construídas a partir da estética realista buscam ofertar um real mais real do que o real, portanto, explícito e contundente, buscando reduzir as mediações e artifícios em seu narrar. A observação deste fenômeno, bem como o estudo das estratégias de autenticação da linguagem audiovisual foram feitas no filme “12 Anos de Escravidão”, de Steve McQueen, vencedor de três Oscars e integrante de uma safra de filmes oscarizados que, em boa parte, foram resultantes da retratação de histórias reais de anônimos.

  16. Nota Fiscal Paulista: principais características percebidas pelos cidadãos paulistas

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    Sérgio Roberto da Silva

    2018-03-01

    Full Text Available Estima-se que 60% dos valores de tributos são sonegados pelo varejo (ROLLI e FERNANDES, 2008. Como resposta o governo Paulista criou o projeto NFP-Nota Fiscal Paulista. Nesse projeto a identificação do contribuinte através do CPF gera créditos (benefícios financeiros ao comprador/contribuinte e assegura o pagamento dos tributos por parte do fornecedor/ comerciante. O objetivo deste trabalho foi identificar as  principais características percebidas pelos cidadãos paulistas com relação ao projeto.  A pesquisa envolveu 132 pessoas em um formulário com 25 assertivas no estilo Likert.  Os resultados indicam que os contribuintes aceitam e participam do projeto. No entanto desconhecem outras características do projeto, por exemplo, doação de créditos a instituições filantrópicas. A maior forma de ressarcimento dos créditos é o depósito em conta corrente. Parte dos contribuintes acredita que as informações referentes aos valores de credito não são repassados a Receita Federal. Dessa forma busca-se contribuir com um estudo sob a ótica dos contribuintes em relação a esse projeto.Nota Fiscal Paulista, análise fatorial, sonegação de tributos

  17. O impacto de um projeto de educação pelo esporte no desenvolvimento infantil

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    Paula Xavier Machado

    Full Text Available Este artigo trata de um estudo com pré-teste (T1 e pós-teste (T2 que avaliou o impacto de um projeto esportivo no desenvolvimento infantil. Participaram 39 estudantes de 6 a 11 anos, matriculados da 1a a 4a séries do Ensino Fundamental de escola pública. Eles foram distribuídos em dois grupos: G1 (24 integrantes do projeto esportivo e G2 (15 crianças do grupo comparativo e os professores dos estudantes também participaram. O impacto foi avaliado pelas diferenças em desempenho escolar, stress, qualidade de vida e avaliação de atitudes acadêmicas e sociais do aluno pelo professor. De T1 para T2, houve aumento significativo no desempenho escolar em G1 e G2. O stress diminuiu para todas as crianças, de T1 para T2, embora seja significativo apenas para G1. A percepção da qualidade de vida diminuiu, entre T1 e T2, para G1 e G2, sendo significativo apenas para G2. Não houve diferença significativa na avaliação dos alunos de G1 e G2, em T1 e T2, pelos professores.

  18. Cartografia do lugar de moradia dos adolescentes com passagem pelo CENSE I de Londrina

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    Claudemir Zulim

    2011-07-01

    Full Text Available Este artigo apresenta os resultados da análise dos dados documentais obtidos na pesquisa realizada no Centro de Socioeducação I - CENSE I, de Londrina, que objetiva identificar, mapear e analisar as condições sócio-espaciais dos bairros de origem dos adolescentes com passagem pela Unidade. O CENSE I é uma unidade de internação provisória por onde passam todos os adolescentes apreendidos em flagrante de ato infracional e encaminhados à autoridade policial competente, que avalia se eles serão liberados para seus familiares ou se de acordo com a gravidade dos fatos que eles sejam ouvidos pelo representante do Ministério Público, enquanto aguardam as entrevistas para a realização das oitivas junto ao Juizado da Vara da Infância e Adolescência. Analisa os dados sobre o lugar de moradia dos adolescentes com passagem pela Unidade no ano de 2009. Utiliza as técnicas de Cartografia e de programas específicos com os quais foram produzidos gráficos e mapas onde são facilmente visualizados os locais de residência desses adolescentes. Verifica que os locais de maior concentração de adolescentes com passagem pelo CENSE I também concentram graves problemas sócio-espaciais, geralmente ligados a ocupações irregulares e em fundos de vale.

  19. Diretrizes para prevenção e tratamento da osteoporose induzida por glicocorticoide Guidelines for the prevention and treatment of glucocorticoid-induced osteoporosis

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    Rosa Maria Rodrigues Pereira

    2012-08-01

    Full Text Available Os glicocorticoides (GC são prescritos por praticamente todas as especialidades médicas, e cerca de 0,5% da população geral do Reino Unido utiliza esses medicamentos. Com o aumento da sobrevida dos pacientes com doenças reumatológicas, a morbidade secundária ao uso dessa medicação representa um aspecto importante que deve ser considerado no manejo de nossos pacientes. As incidências de fraturas vertebrais e não vertebrais são elevadas, variando de 30%-50% em pessoas que usam GC por mais de três meses. Assim, a osteoporose e as fraturas por fragilidade devem ser prevenidas e tratadas em todos os pacientes que iniciarão ou que já estejam em uso desses esteroides. Diversas recomendações elaboradas por várias sociedades internacionais têm sido descritas na literatura, porém não há consenso entre elas. Recentemente, o Americam College of Rheumatology publicou novas recomendações, porém elas são fundamentadas na FRAX (WHO Fracture Risk Assessment Tool para analisar o risco de cada indivíduo e, dessa maneira, não podem ser completamente utilizadas pela população brasileira. Dessa forma, a Comissão de Osteoporose e Doenças Osteometabólicas da Sociedade Brasileira de Reumatologia, em conjunto com a Associação Médica Brasileira e a Associação Brasileira de Medicina Física e Reabilitação, implementou as diretrizes brasileiras de osteoporose induzida por glicocorticoide (OPIG, baseando-se na melhor evidência científica disponível e/ou experiência de experts. DESCRIÇÃO DO MÉTODO DE COLETA DE EVIDÊNCIA: A revisão bibliográfica de artigos científicos desta diretriz foi realizada na base de dados MEDLINE. A busca de evidência partiu de cenários clínicos reais, e utilizou as seguintes palavras-chave (MeSH terms: Osteoporosis, Osteoporosis/chemically induced*= (Glucocorticoids= Adrenal Cortex Hormones, Steroids, Glucocorticoids, Glucocorticoids/administration and dosage, Glucocorticoids/therapeutic use

  20. Clinical evaluation and induced corneal vascularization study by native and anionic collagen membranes in rabbits corneas Avaliação clínica e estudo da vascularização corneal induzida pelas membranas de colágeno nativo e aniônico em córneas de coelhos

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    Thaís Eliane Binotto

    2009-12-01

    treatment are not effective.OBJETIVO: Avaliar os aspectos clínicos e vascularização corneal (VC induzida pelo enxerto interlamelar das membranas de colágeno nativo (MCN e de colágeno aniônico (MCA em córneas de coelhos. MÉTODOS: Um fragmento com 0,25 x 0,25 cm de MCN (grupo 1 e MCA (grupo 2 foi realizado no olho direito (olho tratado por enxertia interlamelar. No olho esquerdo (olho controle foi realizado apenas um túnel estromal. No olho direito (olho controle foi realizado apenas um túnel estromal. Dezesseis coelhos foram utilizados e foram divididos em dois grupos experimentais composto por oito animais cada. A avaliação clínica foi realizada aos 1, 3, 7, 15 e 30 dias de pós-operatório. A análise da vascularização corneal foi realizada após 30 dias pelo Sistema de analisador de imagens Leica Qwin-550®. RESULTADOS: Após 7 dias, a vascularização corneal do limbo em direção central da córnea observada foi de 2,25 ± 0,71 mm (MCN e 1,0 ± 1,69 mm (ACM, respectivamente. Após 15 dias a vascularização corneal aumentou em ambos os grupos (5,25 ± 1,03 mm - MCN; 2,0 ± 2,39 mm - MCA, diminuindo até o 30º dia (2,25 ± 2,10 mm - MCN; 0,75 ± 2,12 mm - MCA. A análise estatística indicou que as médias das distâncias dos vasos do limbo em direção ao enxerto observadas após 7 e 15 dias não diferiram estatisticamente (p=0,17, e 15 e 30 dias de pós-operatório houve tendência a diferir estatisticamente (p=0,09. Os olhos controles não apresentaram nenhuma alteração. CONCLUSÃO: As membranas de colágeno nativo e de colágeno aniônico induzem a vascularização corneal quando aplicadas na córnea de coelhos por meio de enxertia interlamelar, mas membrana de colágeno ativo induz a pequena vascularização corneal quando comparada à membrana de colágeno aniônico. Embora estudos adicionais sejam necessários, os resultados encontrados no presente estudo demonstraram que as membranas de CN e CA possam ser úteis em ceratoplastias. Estas

  1. Projects of SOS FAIM

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    Mees, M.

    1985-01-01

    Full Text Available In Ivory Coast, the freshwater fishculture in rural areas is mainly on a small scale. This type of breeding in ponds (2 to 4 ares yields on an average 3 metric tons of fish/ha/year and represents only an activity with self-consumption of products. The yield in intensive pond fishculture Tilapia nilotica is on an average 6 to 7 metric tons/ha/year but yields bigger than 10 metric tons/ha/year are not uncommon. The intensive fishculture in floating cages, requiring a minor investment but a more improved formation than in fischculture, yields on an average about 30 to 40 kg/m3/year. However the effective development of this activity rests on the resolution of problems like the sufficient fry production, the feeding and the commercialization.

  2. Tratamento homeopático da hepatotoxicose aguda induzida por tetracloreto de carbono em coelhos Homeopatic treatment of acute carbon tetrachloride induced hepatotoxicity in rabbits

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    Maria Cecília Ribeiro Moncorvo

    1998-09-01

    Full Text Available Quinze (15 coelhos (Oryctolagus cuniculus foram submetidos à intoxicação pelo tetracloreto de carbono na dosagem de 0,5 ml/kg de peso corporal, dose única, administrado por sonda gástrica. Foram realizadas as dosagens de alanina amino transferase (ALT, aspartato amino transferase (AST, fosfatase alcalina (FA e gama glutamil transferase (GGT antes e durante o experimento. Vinte e quatro (24 horas após a intoxicação, os coelhos foram divididos aleatoriamente em três grupos de 5 animais. Cada grupo recebeu um tratamento diferente durante 13 dias. O grupo I foi tratado com tetracloreto de carbono diluído na 30ª centesimal hahnemanniana (30 CH, uma vez ao dia. O grupo II recebeu Phosphorus 30 CH, também uma vez ao dia. O grupo III desempenhou o papel de controle, recebendo diariamente uma dose de placebo, pelo mesmo período de tempo que os grupos anteriores. Os resultados das concentrações séricas de ALT, AST, GGT e FA foram submetidos à análise estatística. A variação da concentração de todas as enzimas foi significativa entre os dias, mas nem todas variaram significativamente entre os grupos considerados. O tetracloreto de carbono 30 CH foi capaz de acelerar a recuperação do quadro de hepatite tóxica aguda determinada pela redução dos níveis de ALT. O tratamento com Phosphorus 30 CH mostrou-se incapaz seja de reverter o quadro de hepatite tóxica, seja de acelerar a regeneração hepática.Fithteen (15 New Zeland and Californian rabbits (Oryctolagus cuniculus were submitted to intoxication with carbon tethracloride in a dose 0/0.5 ml/kg, once a day, administred by orogastric sonde. Enzimatic parameters: alanine aminotransferase (ALT, aspartate aminotransferase (AST, alkaline phosphatase and gamma-glutamyltransferase of those rabbits were tested before and during the experiment. After the intoxication, the rabbits were divided in three groups of five animals. Each group received differents treatments during 13 days. The

  3. Proceedings of the Combined 20th International Workshop on Expressiveness in Concurrency and 10th Workshop on Structural Operational Semantics (EXPRESS/SOS 2013, Buenos Aires, Argentina, August 26, 2013)

    NARCIS (Netherlands)

    Borgström, J.; Luttik, B.

    2013-01-01

    This volume contains the proceedings of the Combined 20th International Workshop on Expressiveness in Concurrency and the 10th Workshop on Structural Operational Semantics (EXPRESS/SOS 2013) which was held on 26th August, 2013 in Buenos Aires, Argentina, as an affiliated workshop of CONCUR 2013, the

  4. OS BENEFÍCIOS PERCEBIDOS PELOS PRINCIPAIS STAKEHOLDERS DE UMA REDE DE EMPRESAS

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    Caroline Metzger

    2008-01-01

    Full Text Available O presente artigo retrata um estudo relacionado aos benefícios percebidos pelos principais stakeholders de uma rede de empresas. Para tanto, desenvolveu-se uma pesquisa bibliográfica sobre a pequena empresa, os antecedentes históricos das redes de empresas, os conceitos relacionados, as tipologias e características estruturais, os benefícios proporcionados pelas redes e apresentou-se o Programa Redes de Cooperação no Estado do Rio Grande do Sul. Descreveram-se também os conceitos de stakeholders, as suas classificações e os benefícios do relacionamento destes grupos. Considerando este contexto, o objetivo deste artigo foi identificar e analisar os benefícios percebidos pelos principais stakeholders envolvidos com a rede de cooperação Hipereletro. A metodologia utilizada foi o estudo de caso a partir dos dados coletados através da pesquisa de campo, por meio de um questionário aplicado a quatro grupos envolvidos com a Hipereletro. Após a análise dos dados, identificaram-se os benefícios percebidos por cada stakeholder. Os associados perceberam benefícios nas ações de marketing, nas negociações em conjunto e no relacionamento entre as empresas, destacam-se as promoções realizadas pelo grupo, obtenção de melhores preços nas compras e troca de informações, conhecimentos e experiências. Já os funcionários apontaram a melhora na apresentação da empresa, realização de eventos e a troca de informações entre as lojas. Para os fornecedores, os primeiros reflexos da parceria com a rede foram o aumento das vendas, a garantia de pagamento e a fidelidade das empresas. E, quanto aos clientes, destacaram-se a percepção em relação à mudança da fachada da loja, as promoções e a diferença nos preços dos produtos. Pela pesquisa, concluiu-se que todos percebem benefícios ao se relacionarem com a rede, alguns mais, outros menos. Porém, devido ao pouco tempo de existência da Hipereletro, todos acreditam no

  5. Aerocistite aguda induzida por tioglicolato, lipolisacarídeo e Aeromona hydrophila inativada em Piaractus mesopotamicus: efeitos hematológicos

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    Julieta Rodini Engrácia de Moraes

    2012-10-01

    Full Text Available Os efeitos da injeção de tioglicolato, lipolissacarídio de Escherichia coli e Aeromonas hydrophila inativada na bexiganatatória de pacus, Piaractus mesopotamicus (Characidae foram avaliados quanto às respostas de células vermelhas,leucócitos e trombócitos do sangue. Ensaios quantitativos de eritrócitos, leucócitos e trombócitos foram realizados6, 24 e 48 h após os estímulos e comparados com peixes que receberam solução salina 0,65% pela mesma via. Peixes inoculados com A. hydrophila apresentaram redução do número de eritrócitos e da taxa de hemoglobina enquanto ohematócrito aumentou 6 h após o estímulo. Os resultados mostraram que o tioglicolato e o LPS também induziram redução da hemoglobina e aumento do hematócrito. A contagem de trombócitos diminuiu 6 h após a inoculação deA. hydrophila inativada e aumentou 48 horas após a injeção de LPS. A contagem de leucócitos aumentou 6 h após ainoculação de A. hydrophila enquanto a de linfócitos a leucócitos granulares PAS positivos (PAS_LG diminuiu 24 hdepois. Peixes injetados com tioglicolato o LPS apresentaram aumento do número de LG_PAS em relação aos inoculadoscom A. hydrophila inativada ou grupo controle. A contagem de monócitos não foi afetada pelos diferentes agentes.

  6. Auto cuidado no tratamento pelo método de Ilizarov: um estudo de caso

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    Marisa Toshiko Ono Tashiro

    1995-03-01

    Full Text Available As autoras realizaram um estudo de caso com aplicação do modelo de auto cuidado de Dorothea Orem, no tratamento pelo método de Ilizarov, utilizando cuidados e orientações específicas para a recuperação e prevenção de complicações. A operacionalização do estudo constou de procedimentos relativos às demandasdecuida dos universais e terapêuticos, durante a hospitalização. As demandas foram detectadas no pré-operatório e os cuidados desenvolvidos no pós-operatório, facilitando ao paciente o aprendizado e a realização dos procedimentos necessários a dar continuidade dos mesmos no seu domicílio.

  7. Infecção pelo HIV: descritores de mortalidade em pacientes hospitalizados

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    Luiz Claudio Santos Thuler

    1998-12-01

    Full Text Available OBJETIVO: Estudar os descritores clínico-epidemiológicos da mortalidade em pacientes internados por condições clínicas associadas à infecção pelo HIV. MÉTODO: Estudo retrospectivo de todos os pacientes adultos hospitalizados em 1990, 1992 e 1994 em hospital universitário. Os resultados foram descritos como números absolutos, percentagens e médias, sendo a significância estatística entre as diferenças avaliada pelos testes do qui-quadrado, exato de Fisher ou t de Student, conforme o caso. Um modelo de regressão logística foi elaborado visando a identificar os principais fatores associados ao risco de evolução para o óbito. RESULTADOS: Foram incluídos no estudo 240 pacientes. Entre 1990 e 1994 a idade média dos pacientes aumentou de 35,0 para 36,9 anos, a razão entre os sexos masculino e feminino caiu de 9,8 para 2,0, a proporção de não brancos cresceu de 18,5 para 41,3 e registrou-se um aumento do tempo médio entre a descoberta da infecção pelo HIV e a hospitalização de 0,7 para 2,5 anos. Observou-se a redução do número médio de dias de hospitalização de 31,3 para 25,3 e aumento da proporção de pacientes em acompanhamento ambulatorial de 47,8 para 83,3%. As infecções respiratórias representaram a principal causa de hospitalização (58% e as infecções oportunistas apresentadas com maior freqüência foram: candidíase oral (27,1%, tuberculose (18,3%, pneumonia por Pneumocystis carinii (15,4% e neurotoxoplasmose (10,4%. Na análise multivariada, apenas o tempo de hospitalização menor ou igual a 7 dias (Odds Ratio [OR]=3,88; p=0,02 e a ausência de acompanhamento ambulatorial (OR=3,29; p=0,01 mostraram-se associados a um maior risco de evolução para óbito. CONCLUSÃO: O conhecimento dos fatores associados a um risco aumentado de morte pode ser útil na tomada de decisão frente a pacientes hospitalizados com infecção pelo HIV.

  8. Facilitação da memória olfativa pelo etanol em ratos

    OpenAIRE

    Prediger, Rui Daniel Schröder

    2002-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas. Programa de Pós-Graduação em Farmacologia. Um crescente número de estudos em humanos, e em outros animais, têm revelado uma facilitação dos processos de memória pelo etanol quando este é administrado imediatamente após o treino e os indivíduos são posteriormente testados sem a ação da droga. O objetivo do presente trabalho foi avaliar os efeitos de uma única dose de etanol (0,5; 1,0 e 2,0 g/Kg),...

  9. Democracia digital? Os usos da Internet pelos sindicatos da saúde

    OpenAIRE

    Marques Alves, P.

    2015-01-01

    Os movimentos sindicais dos países capitalistas desenvolvidos vêm enfrentando uma crise profunda desde os anos 70. As suas causas são múltiplas, nelas se mesclando fatores que lhes são exógenos com outros endógenos, remetendo para a sua burocratização. Visando revitalizar-se, eles têm vindo a implementar ações diversificadas, onde se conta a adoção das TIC e da Internet. Este artigo apresenta os resultados de um estudo sobre a adoção e os usos da Internet pelos sindicatos da saúde. Visou-se c...

  10. A GESTÃO DO TERRITÓRIO PELO CAPITAL SUCROALCOOLEIRO NO NORTE DO PARANÁ

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    Marcos Antonio de Souza

    2013-02-01

    Full Text Available Toda produção econômica possui uma determinada territorialidade, cuja gestão deste território está orientada no desenvolvimento de estratégias que primem pela rentabilidade da produção, prioritariamente em patamares diferenciais. Partindo-se da premissa de que o território não é uma mera localização, a agroindústria canavieira não se localiza pura e simplesmente em uma base física qualquer. Antes, há uma seletividade espacial marcada pelas disputas territoriais e a partir destas há o exercício do controle sob os elementos necessários para realizar a sua produção, subordinando a terra, a força de trabalho, os recursos naturais ao seu negócio que prima pela reprodução ampliada do capital. Trata-se na verdade de um conjunto de estratégias espaciais, onde o poder econômico ao controlar o território, reserva para si a gestão deste no âmbito do pacto social engendrado pelos grupos hegemônicos, resultando em um território gerido e ordenado pelo capital que aí se territorializou. Nesse contexto, este trabalho busca apresentar as principais estratégias espaciais dos agroindustriais do setor canavieiro desenvolvidas nas últimas três décadas no Norte do Paraná.

  11. Toxicidade ocular causada pelo tamoxifeno: relato de caso Ocular toxicity caused by tamoxifen: case report

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    Eliane Terumi Inada

    2005-08-01

    Full Text Available O objetivo desse trabalho é relatar um caso de toxicidade ocular pelo tamoxifeno. Para isso, aferiu-se a melhor acuidade visual corrigida de ambos os olhos em tabela de Snellen. Foram realizados biomicroscopia do segmento anterior, refração, oftalmoscopia, angiofluoresceinografia e retinografia numa paciente de 63 anos, sexo feminino, cor branca, em uso de tamoxifeno 20 mg/dia há 4 anos, com acuidade visual corrigida de 20/70 e 20/40. A biomicroscopia do segmento anterior apresentava ceratopatia verticilata e catarata nuclear e cortical posterior de 1+/4 em ambos os olhos. À oftalmoscopia, foi verificado alteração do brilho macular de ambos os olhos. E a angiofluoresceinografia mostrou hiperfluorescência na área macular em fase precoce (defeito em janela. Relata-se um caso de ceratopatia e maculopatia causadas pelo tamoxifeno.To report tamoxifen ocular toxicity. The best visual acuity was measured in both eyes with Snellen chart, slit-lamp examination of anterior segment, refraction, dilated fundus examination, fluorescein angiography and retinography in a 63-year-old patient, female, white, using tamoxifen 20 mg/day for 4 years, with 20/70 and 20/40 corrected visual acuity. The anterior segment examination showed corneal linear subepithelial opacity inferior to the visual axis and nuclear and posterior cortical cataract (1+/4 in both eyes. Fundus examination showed alteration of macular color in both eyes. Fluorescein angiography presented hyperfluorescence in the macular area at an early phase (window defect. Report of keratopathy and maculopathy caused by tamoxifen.

  12. O adoecimento pelo cancer de laringe The illness narrative for larynx cancer

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    Márcia Maria Fontão Zago

    2001-06-01

    Full Text Available Com a finalidade de aprimorar a assistência ao laringectomizado, este estudo teve o objetivo de compreender o adoecimento pelo câncer de laringe na visão do paciente e sua família. Participaram do estudo 14 pacientes laringectomizados e familiares, em diferentes períodos pós-operatórios. Na análise das narrativas identificamos categorias que foram interpretadas sob a visão antropológica da cultura. Na interpretação dos participantes, o processo é permeado pelo sofrimento, pela busca de estratégias de enfrentamento, pela valorização de sobreviver à cirurgia, ou seja, pressupostos culturais que diferem do modelo biomédico dos profissionais e que devem ser considerados na sua reabilitação.With the purpose of improving the care of laryngectomized patients, the aim is to understand the illness experience of patients and families of having larynx cancer. The participants were 14 laryngectomized patients and their families, in different post-surgical periods. The narrative analysis searched for categories which were understood on the basis of an anthropological focusing the culture. The understanding that the participants had of the illness experience of having a larynx cancer shows us that this process is one of suffering, of searching of strategies of coping, of evaluating the survival to the surgical treatment, which were based on the cultural system, which is different from the biomedical model of the health professionals and they must be considered in the approach for the rehabilitation.

  13. AT1-receptor mediated vascular damage in myocardium, kidneys and liver in rats Lesão vascular mediada pelo receptor AT1 esses efeitos em miocárdio, rins e fígado de ratos

    Directory of Open Access Journals (Sweden)

    Maria do Carmo Fernandez Vailati

    2010-07-01

    Full Text Available The systemic aspect of vascular damage induced by angiotensin II (ANG II has been poorly explored in the literature. Considering the presence of ANG II and its specific receptor AT1, in several organs, all tissues might be potentially affected by its effects. The aims of this study were: To evaluate the early histological changes in the heart, liver and kidneys, produced by ANG II infusion, to evaluate the protective effect of losartan. Wistar rats were distributed into three groups: control (no treatment, treated with ANG II, and treated with ANG II + losartan. ANG II was continuously infused over 72 hours by subcutaneous osmotic pumps. Histological sections of the myocardium, kidneys and liver were stained and observed for the presence of necrosis. There were ANG II-induced perivascular inflammation and necrosis of the arteriolar wall in the myocardium, kidney, and liver by, which were partially prevented by losartan. There was no significant correlation between heart and kidney damage. Tissue lesion severity was lower than that of vascular lesions, without statistical difference between groups. ANG II causes vascular injury in the heart, kidneys and liver, indicating a systemic vasculotoxic effect; the mechanisms of damage/protection vary depending on the target organ; perivascular lesions may occur even when anti-hypertensive doses of losartan are used.O aspecto sistêmico da lesão vascular induzida pela angiotensina II (ANG II tem sido pouco explorada na literatura. Considerando a presença de ANG II e de seu receptor AT1 em diversos órgãos, todos os tecidos poderiam ser potencialmente afetados por esses efeitos. Os objetivos deste estudo foram: avaliar as alterações histológicas iniciais no coração, fígado e rins, produzidas pela infusão de ANG II, e avaliar o efeito protetor do losartan. Ratos Wistar foram divididos em três grupos: controle (sem tratamento, tratados com ANG II, e tratados com ANG II + losartan. A ANG II foi

  14. Blocking by the carcinogen, L-ethionine, of SOS functions in a tif-1 mutant of Escherichia coli B/r

    International Nuclear Information System (INIS)

    Wiesner, R.; Troll, W.

    1981-01-01

    In Escherichia coli, DNA damage by carcinogenic agents results in the coordinate expression of a diversity of functions (SOS functions), many of which are thermally inducible without any damage to DNA in a tif-1 mutant. These include prophage induction, filamentous growth, and an error-prone DNA repair activity, which is responsible for ultraviolet-induced mutagenesis. Ethionine ca