Salmon Site Remedial Investigation Report, Exhibit 5

International Nuclear Information System (INIS)

1999-01-01

This Salmon Site Remedial Investigation Report provides the results of activities initiated by the U.S. Department of Energy (DOE) to determine if contamination at the Salmon Site poses a current or future risk to human health and the environment. These results were used to develop and evaluate a range of risk-based remedial alternatives. Located in Lamar County, Mississippi, the Salmon Site was used by the U.S. Atomic Energy Commission (predecessor to the DOE) between 1964 and 1970 for two nuclear and two gas explosions conducted deep underground in a salt dome. The testing resulted in the release of radionuclides into the salt dome. During reentry drilling and other site activities, liquid and solid wastes containing radioactivity were generated resulting in surface soil and groundwater contamination. Most of the waste and contaminated soil and water were disposed of in 1993 during site restoration either in the cavities left by the tests or in an injection well. Other radioactive wastes were transported to the Nevada Test Site for disposal. Nonradioactive wastes were disposed of in pits at the site and capped with clean soil and graded. The preliminary investigation showed residual contamination in the Surface Ground Zero mud pits below the water table. Remedial investigations results concluded the contaminant concentrations detected present no significant risk to existing and/or future land users, if surface institutional controls and subsurface restrictions are maintained. Recent sampling results determined no significant contamination in the surface or shallow subsurface. The test cavity resulting from the experiments is contaminated and cannot be economically remediated with existing technologies. The ecological sampling did not detect biological uptake of contaminants in the plants or animals sampled. Based on the current use of the Salmon Site, the following remedial actions were identified to protect both human health and the environment: (1) the

WAG 2 remedial investigation and site investigation site-specific work plan/health and safety checklist for the soil and sediment task

International Nuclear Information System (INIS)

Holt, V.L.; Burgoa, B.B.

1993-12-01

This document is a site-specific work plan/health and safety checklist (WP/HSC) for a task of the Waste Area Grouping 2 Remedial Investigation and Site Investigation (WAG 2 RI ampersand SI). Title 29 CFR Part 1910.120 requires that a health and safety program plan that includes site- and task-specific information be completed to ensure conformance with health- and safety-related requirements. To meet this requirement, the health and safety program plan for each WAG 2 RI ampersand SI field task must include (1) the general health and safety program plan for all WAG 2 RI ampersand SI field activities and (2) a WP/HSC for that particular field task. These two components, along with all applicable referenced procedures, must be kept together at the work site and distributed to field personnel as required. The general health and safety program plan is the Health and Safety Plan for the Remedial Investigation and Site Investigation of Waste Area Grouping 2 at the Oak Ridge National Laboratory, Oak Ridge, Tennessee (ORNL/ER-169). The WP/HSCs are being issued as supplements to ORNL/ER-169

Planning for investigation and evaluation of potential repository sites in Sweden

International Nuclear Information System (INIS)

Almen, K.E.; Stroem, A.

1998-01-01

The present stage of siting of the Swedish Deep Repository for spent nuclear fuel involves general siting studies on national and regional scales and feasibility studies on a municipal scale. Based on these studies, two areas will be selected for surface-based site investigations. The geoscientific site information will be used in the site evaluation process, in which performance and safety assessments and design studies are the major activities, in combination with geoscientific characterization. The safety report and EIA document from the site investigation stage will be the most important documents in the application for the siting permit and the permit to construct the deep repository. Detailed characterization will then verify the suitability of the selected site. The programme for geoscientific site investigations is based on experience from more than 20 years of field studies in several SKB projects, such as the Study Site Investigations , the Stripa Project, and the Aespoe Hard Rock Laboratory. The strategies and methodologies developed, implemented and verified within the Aespoe HRL are a very important source of information and know-how for the development of the site investigation programme. The investigations will produce geoscientific models that include all information needed to analyze the long-term safety of a deep repository located in and adapted to the geological conditions of the rock. The type of geoscientific information needed for performance and safety assessment, layout and design, environmental studies and for fundamental geoscientific understanding has been specified and compiled in a 'parameter' report. The general strategy is that performance assessment, layout and design studies will be conducted in parallel with the geoscientific investigations. Information will be transferred at logical occasions, when decisions have to be taken and when feedback is desirable for new investigation steps. The role of the geoscientific evaluation is to

WAG 2 remedial investigation and site investigation site-specific work plan/health and safety checklist for the sediment transport modeling task

International Nuclear Information System (INIS)

Holt, V.L.; Baron, L.A.

1994-05-01

This site-specific Work Plan/Health and Safety Checklist (WP/HSC) is a supplement to the general health and safety plan (HASP) for Waste Area Grouping (WAG) 2 remedial investigation and site investigation (WAG 2 RI ampersand SI) activities [Health and Safety Plan for the Remedial Investigation and Site Investigation of Waste Area Grouping 2 at the Oak Ridge National Laboratory, Oak Ridge, Tennessee (ORNL/ER-169)] and provides specific details and requirements for the WAG 2 RI ampersand SI Sediment Transport Modeling Task. This WP/HSC identifies specific site operations, site hazards, and any recommendations by Oak Ridge National Laboratory (ORNL) health and safety organizations [i.e., Industrial Hygiene (IH), Health Physics (HP), and/or Industrial Safety] that would contribute to the safe completion of the WAG 2 RI ampersand SI. Together, the general HASP for the WAG 2 RI ampersand SI (ORNL/ER-169) and the completed site-specific WP/HSC meet the health and safety planning requirements specified by 29 CFR 1910.120 and the ORNL Hazardous Waste Operations and Emergency Response (HAZWOPER) Program Manual. In addition to the health and safety information provided in the general HASP for the WAG 2 RI ampersand SI, details concerning the site-specific task are elaborated in this site-specific WP/HSC, and both documents, as well as all pertinent procedures referenced therein, will be reviewed by all field personnel prior to beginning operations

[Organising an investigation site: a national training reference document].

Science.gov (United States)

Cornu, Catherine; David, Frédérique; Duchossoy, Luc; Hansel-Esteller, Sylvie; Bertoye, Pierre-Henri; Giacomino, Alain; Mouly, Stéphane; Diebolt, Vincent; Blazejewski, Sylvie

2014-01-01

Several surveys have shown a declining performance of French investigators in conducting clinical trials. This is partly due to insufficient and heterogeneous investigator training and site organisation. A multidisciplinary group was set up to propose solutions. We describe the tools developed to improve study site organisation. This working group was made up of clinical research experts from academia, industry, drug regulatory authorities, general practice, and consulting. Methods and tools were developed to improve site organisation. The proposed tools mainly focus on increasing investigators' awareness of their responsibilities, their research environment, the importance of a thorough feasibility analysis, and the implementation of active patient recruitment strategies. These tools should be able to improve site organisation and performances in conducting clinical trials. © 2014 Société Française de Pharmacologie et de Thérapeutique.

Site investigations for final disposal of high-level nuclear waste

International Nuclear Information System (INIS)

Aeikaes, T.; Laine, T.

1982-12-01

Research concerning disposal of high-level nuclear waste of the Industrial Power Company Ltd has focused on deep underground disposal in Finnish precambrian bedrock. The present target is to have a repository for high-level waste in operation by 2020. Selection of the repository site is based on site investigations. In addition to geosciences, selection of appropriate site includes many branches of studies; engineering, safety analysis, ecology, transport, demography etc. The investigations required for site selection for high-level waste have been arranged in a sequence of four phases. The aim of the phases is that investigations become more and more detailed as the selection process continues. Phase I of the investigations is the characterization of potential areas. This comprises establishment of criteria for site selection and identification of areas that meet selection criteria. Objective of these studies is to determine areas for phase II field investigations. The studies are largely made by reviewing existing data and remote-sensing techniques. Phase II field investigations will be undertaken between 1986-1992. The number of potential candidates for repository site is reduced to few preferred areas by preceeding generic study. The site selection process culminates in phase III in site confirmation studies carried out at 2...3 most suitable sites during 1992-2010. This is then followed by phase IV, which comprises very detailed investigations at the selected site. An alternative for these investigations is to undertake them by using pilot shaft and drifts. Active development is taking place in all phases concerning investigation methods, criteria, parameters, data processing and modelling. The applicability of the various investigation methods and techniques is tested in a deep borehole in phase I. The co-operation with countries with similar geological conditions makes it possible to compare results obtained by different techniques

Geoscientific programme for investigation and evaluation of sites for the deep repository

International Nuclear Information System (INIS)

2000-08-01

is ensured for the planned deep repository based on reported investigation results and the proposed repository layout. The safety assessment includes analyses of technical, hydraulic, mechanical and chemical processes around a deep repository as well as calculations of radionuclide transport. Data after completed site investigation should serve as a basis for an assessment of at least the same scope as the SR 97 safety assessment. The methodology in SR 97 hereby comprises a basis for the methodology to be used in future safety assessments. The need for data is the primary factor that will guide field activities during the site investigations. What is possible and suitable to do is, however, also determined to a great extent by environmental conditions on the site. Measurements will be made from the air, from the ground and in boreholes. To enable the measurements to be performed quickly and conveniently, some small roads may have to be built and power lines run up to the drilling sites. Drilling is the 'heaviest' activity during the site investigations. Protected areas will be avoided wherever possible. The same applies to other areas that may be sensitive to disturbances, such as breeding areas for unusual bird species and localities with rare plants. With good knowledge concerning the study site's flora and fauna, the field activities can be adapted so that any disturbances will be limited. The initial site investigations are estimated to take around 2 years. The time required is projected to be different for the different sites because the conditions and level of knowledge are different. To enable the resources to be utilized efficiently, the timetables for the investigations at the different sites will be somewhat staggered. The complete site investigations include several drilling stages, where 3-4 deep core boreholes are drilled simultaneously. This is followed by measurements and evaluation of the results. Each drilling stage is estimated to take around one

Geoscientific programme for investigation and evaluation of sites for the deep repository

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-08-01

-term safety is ensured for the planned deep repository based on reported investigation results and the proposed repository layout. The safety assessment includes analyses of technical, hydraulic, mechanical and chemical processes around a deep repository as well as calculations of radionuclide transport. Data after completed site investigation should serve as a basis for an assessment of at least the same scope as the SR 97 safety assessment. The methodology in SR 97 hereby comprises a basis for the methodology to be used in future safety assessments. The need for data is the primary factor that will guide field activities during the site investigations. What is possible and suitable to do is, however, also determined to a great extent by environmental conditions on the site. Measurements will be made from the air, from the ground and in boreholes. To enable the measurements to be performed quickly and conveniently, some small roads may have to be built and power lines run up to the drilling sites. Drilling is the 'heaviest' activity during the site investigations. Protected areas will be avoided wherever possible. The same applies to other areas that may be sensitive to disturbances, such as breeding areas for unusual bird species and localities with rare plants. With good knowledge concerning the study site's flora and fauna, the field activities can be adapted so that any disturbances will be limited. The initial site investigations are estimated to take around 2 years. The time required is projected to be different for the different sites because the conditions and level of knowledge are different. To enable the resources to be utilized efficiently, the timetables for the investigations at the different sites will be somewhat staggered. The complete site investigations include several drilling stages, where 3-4 deep core boreholes are drilled simultaneously. This is followed by measurements and evaluation of the results. Each drilling stage is estimated to

Site investigations for repositories for solid radioactive wastes in shallow ground

International Nuclear Information System (INIS)

1982-01-01

This report provides an overview and technical guidelines for investigations on a national level for the selection and confirmation of a repository site that will provide adequately safe performance for disposal of solid radioactive wastes that are low- or intermediate-level and short-lived. It also provides basic information on technical activities to be undertaken and on techniques that are available for such investigations in the various steps in selecting suitable sites. The report supplements the information given in Shallow Ground Disposal of Radioactive Wastes: A Guidebook, IAEA Safety Series No. 53 (1981). This report focuses mainly on different aspects of earth sciences and the various investigative techniques relative to earth sciences that may be necessary for site investigations. Some major related studies in other fields are discussed briefly. It is assumed that no previous investigations have been undertaken, and the report proceeds through area site selection to the stage when the site is confirmed as suitable for a waste repository

Site investigations for repositories for solid radioactive wastes in shallow ground

Energy Technology Data Exchange (ETDEWEB)

1982-01-01

This report provides an overview and technical guidelines for investigations on a national level for the selection and confirmation of a repository site that will provide adequately safe performance for disposal of solid radioactive wastes that are low- or intermediate-level and short-lived. It also provides basic information on technical activities to be undertaken and on techniques that are available for such investigations in the various steps in selecting suitable sites. The report supplements the information given in Shallow Ground Disposal of Radioactive Wastes: A Guidebook, IAEA Safety Series No. 53 (1981). This report focuses mainly on different aspects of earth sciences and the various investigative techniques relative to earth sciences that may be necessary for site investigations. Some major related studies in other fields are discussed briefly. It is assumed that no previous investigations have been undertaken, and the report proceeds through area site selection to the stage when the site is confirmed as suitable for a waste repository.

Corrective Action Investigation Plan for Corrective Action Unit 137: Waste Disposal Sites, Nevada Test Site, Nevada

International Nuclear Information System (INIS)

Wickline, Alfred

2005-01-01

This Corrective Action Investigation Plan (CAIP) contains project-specific information including facility descriptions, environmental sample collection objectives, and criteria for conducting site investigation activities at Corrective Action Unit (CAU) 137: Waste Disposal Sites. This CAIP has been developed in accordance with the ''Federal Facility Agreement and Consent Order'' (FFACO) (1996) that was agreed to by the State of Nevada, the U.S. Department of Energy (DOE), and the U.S. Department of Defense. Corrective Action Unit 137 contains sites that are located in Areas 1, 3, 7, 9, and 12 of the Nevada Test Site (NTS), which is approximately 65 miles (mi) northwest of Las Vegas, Nevada (Figure 1-1). Corrective Action Unit 137 is comprised of the eight corrective action sites (CASs) shown on Figure 1-1 and listed below: (1) CAS 01-08-01, Waste Disposal Site; (2) CAS 03-23-01, Waste Disposal Site; (3) CAS 03-23-07, Radioactive Waste Disposal Site; (4) CAS 03-99-15, Waste Disposal Site; (5) CAS 07-23-02, Radioactive Waste Disposal Site; (6) CAS 09-23-07, Radioactive Waste Disposal Site; (7) CAS 12-08-01, Waste Disposal Site; and (8) CAS 12-23-07, Waste Disposal Site. The Corrective Action Investigation (CAI) will include field inspections, radiological surveys, geophysical surveys, sampling of environmental media, analysis of samples, and assessment of investigation results, where appropriate. Data will be obtained to support corrective action alternative evaluations and waste management decisions. The CASs in CAU 137 are being investigated because hazardous and/or radioactive constituents may be present in concentrations that could potentially pose a threat to human health and the environment. Existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives for the CASs. Additional information will be generated by conducting a CAI before evaluating and selecting corrective action

Oskarshamn site investigation. Monitoring of shallow groundwater chemistry 2009

International Nuclear Information System (INIS)

Ericsson, Ulf

2010-06-01

In 2009 sampling of shallow ground water in water wells in soil has been performed in a regular programme at eight sites within the site investigation area at Oskarshamn. The purpose of the activity is to monitor (long term observation) and characterise the shallow ground water in the site investigation area. Some physical and chemical parameters were measured directly in the field but most parameters were analysed at different laboratories. The ground water sampling activity consisted of one programme, chemical programme class 5 (reduced). The large number of sites and parameters analysed have generated a large amount of data, which will later be used for advanced analysis and modelling. In this report the evaluation aims to give a simple overview of the results and to describe the quality of the data sampled 2009. As an addition radon activity was measured at fourteen sites in the area. Seven of these sites were the same as in the regular programme. The results showed a large variation between the wells. The concentrations of major ions and conductivity ranged from low to high or very high values. The concentration of HCO 3 also varied extensively throughout the investigation area, but since the concentrations were above 60 mg/l in most wells the results indicate a good ground water quality with respect to acidification. The concentration of heavy metals and trace elements also varied. High concentration of Pb in some of the wells indicated pollution. Since Pb had a similar relation to Al as most other elements it was argued that high concentrations of lead probably can be explained by the natural composition of minerals within the site investigation area. The ratio of δ 18 O showed a good relationship with the conductivity. The activity of tritium ( 3 H) was markedly lower in two of the wells. This might be an indication of older groundwater in these wells. The average hydrogen isotope ratio of deuterium (δ 2 H) varied with similar values in most wells. The �

Oskarshamn site investigation. Monitoring of shallow groundwater chemistry 2009

Energy Technology Data Exchange (ETDEWEB)

Ericsson, Ulf (Medins Biologi AB, Moelnlycke (Sweden))

2010-06-15

In 2009 sampling of shallow ground water in water wells in soil has been performed in a regular programme at eight sites within the site investigation area at Oskarshamn. The purpose of the activity is to monitor (long term observation) and characterise the shallow ground water in the site investigation area. Some physical and chemical parameters were measured directly in the field but most parameters were analysed at different laboratories. The ground water sampling activity consisted of one programme, chemical programme class 5 (reduced). The large number of sites and parameters analysed have generated a large amount of data, which will later be used for advanced analysis and modelling. In this report the evaluation aims to give a simple overview of the results and to describe the quality of the data sampled 2009. As an addition radon activity was measured at fourteen sites in the area. Seven of these sites were the same as in the regular programme. The results showed a large variation between the wells. The concentrations of major ions and conductivity ranged from low to high or very high values. The concentration of HCO{sub 3} also varied extensively throughout the investigation area, but since the concentrations were above 60 mg/l in most wells the results indicate a good ground water quality with respect to acidification. The concentration of heavy metals and trace elements also varied. High concentration of Pb in some of the wells indicated pollution. Since Pb had a similar relation to Al as most other elements it was argued that high concentrations of lead probably can be explained by the natural composition of minerals within the site investigation area. The ratio of delta18O showed a good relationship with the conductivity. The activity of tritium (3H) was markedly lower in two of the wells. This might be an indication of older groundwater in these wells. The average hydrogen isotope ratio of deuterium (delta2H) varied with similar values in most wells

When is there sufficient information from the Site Investigations?

International Nuclear Information System (INIS)

Andersson, Johan; Munier, Raymond; Stroem, Anders; Soederbaeck, Bjoern; Almen, Karl-Erik; Olsson, Lars

2004-04-01

can be used in the decision whether and how to continue the investigation. Highlight uncertainty evaluation of aspects of the Site Description judged to be critical to the decision of investigation sufficiency ('uncertainty of key aspects') and to help substantiate how these uncertainties should be explored such that they can be used in the decision analysis. In addressing these objectives the report describes some formal decision analyses tools, assessed potential feedback from Safety Assessment and Rock Engineering, explores some key uncertainty in the Site Description and also explores an example where the decision analyses tools are applied. Based on this work it is generally concluded that the question When to stop the site investigation is a decision problem. In simple words the site investigations should stop when the expected net gain of further investigations is zero or negative, where gain is related to the total cost, which includes several factors besides the direct monetary outlay. Furthermore, this decision perspective should be applied not only in determining the sufficiency of the programme, but also when optimising the remaining parts of the ongoing programme. In particular, it needs to be understood that the Site Investigation is only a step towards the ultimate decision of disposing waste and eventually sealing a repository. The cost and value of information obtained in the Site Investigation Phase, needs to be weighted against the costs and value of obtaining information during later stages. All issues need not be resolved during the Site Investigation Phase. Some could be much better handled later, e.g. during the construction and detailed investigation phase. More specifically, it is recommended to: to actively solicit feedback from Safety Assessment and Repository Engineering, keep track of the uncertainty evaluation of the key aspects of the Site Descriptive Models, develop the Underground Characterisation Programme as this is the next step

When is there sufficient information from the Site Investigations?

Energy Technology Data Exchange (ETDEWEB)

Andersson, Johan [JA Streamflow AB, Aelvsjoe (Sweden); Munier, Raymond; Stroem, Anders; Soederbaeck, Bjoern [Swedish Nuclear Fuel and Waste Management Co., Stockholm (Sweden); Almen, Karl-Erik [KEA Geo-konsult (Sweden); Olsson, Lars [Geostatistik AB, Tumba (Sweden)

2004-04-01

can be used in the decision whether and how to continue the investigation. Highlight uncertainty evaluation of aspects of the Site Description judged to be critical to the decision of investigation sufficiency ('uncertainty of key aspects') and to help substantiate how these uncertainties should be explored such that they can be used in the decision analysis. In addressing these objectives the report describes some formal decision analyses tools, assessed potential feedback from Safety Assessment and Rock Engineering, explores some key uncertainty in the Site Description and also explores an example where the decision analyses tools are applied. Based on this work it is generally concluded that the question When to stop the site investigation is a decision problem. In simple words the site investigations should stop when the expected net gain of further investigations is zero or negative, where gain is related to the total cost, which includes several factors besides the direct monetary outlay. Furthermore, this decision perspective should be applied not only in determining the sufficiency of the programme, but also when optimising the remaining parts of the ongoing programme. In particular, it needs to be understood that the Site Investigation is only a step towards the ultimate decision of disposing waste and eventually sealing a repository. The cost and value of information obtained in the Site Investigation Phase, needs to be weighted against the costs and value of obtaining information during later stages. All issues need not be resolved during the Site Investigation Phase. Some could be much better handled later, e.g. during the construction and detailed investigation phase. More specifically, it is recommended to: to actively solicit feedback from Safety Assessment and Repository Engineering, keep track of the uncertainty evaluation of the key aspects of the Site Descriptive Models, develop the Underground Characterisation Programme as this is the

Remedial Investigation of Hanford Site Releases to the Columbia River

International Nuclear Information System (INIS)

Lerch, J.A.

2009-01-01

In south-central Washington State, the Columbia River flows through the U.S. Department of Energy Hanford Site. A primary objective of the Hanford Site cleanup mission is protection of the Columbia River, through remediation of contaminated soil and groundwater that resulted from its weapons production mission. Within the Columbia River system, surface water, sediment, and biota samples related to potential Hanford Site hazardous substance releases have been collected since the start of Hanford operations. The impacts of Hanford Site hazardous substance releases to the Columbia River in areas upstream, within, and downstream of the Hanford Site boundary have been previously investigated as mandated by the U.S. Department of Energy requirements under the Atomic Energy Act. The impacts are now being assessed under the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 via a remedial investigation. The Remedial Investigation Work Plan for Hanford Site Releases to the Columbia River has been developed and issued to initiate the remedial investigation. The work plan establishes a phased approach to characterize contaminants, assess current risks, and determine whether or not there is a need for any cleanup actions. Field investigation activities began in October 2008 and are anticipated to continue into Fall 2009 over a 120 mile stretch of the Columbia River. Information gained from performing this remedial investigation will ultimately be used to help make final regulatory decisions for cleaning up Hanford Site contamination that exists in and along the Columbia River. (authors)

Site safety plan for Lawrence Livermore National Laboratory CERCLA investigations at site 300. Revision 2

Energy Technology Data Exchange (ETDEWEB)

Kilmer, J.

1997-08-01

Various Department of Energy Orders incorporate by reference, health and safety regulations promulgated by the Occupational Safety and Health Administration (OSHA). One of the OSHA regulations, 29 CFR 1910.120, Hazardous Waste Operations and Emergency Response, requires that site safety plans are written for activities such as those covered by work plans for Site 300 environmental investigations. Based upon available data, this Site Safety Plan (Plan) for environmental restoration has been prepared specifically for the Lawrence Livermore National Laboratory Site 300, located approximately 15 miles east of Livermore, California. As additional facts, monitoring data, or analytical data on hazards are provided, this Plan may need to be modified. It is the responsibility of the Environmental Restoration Program and Division (ERD) Site Safety Officer (SSO), with the assistance of Hazards Control, to evaluate data which may impact health and safety during these activities and to modify the Plan as appropriate. This Plan is not `cast-in-concrete.` The SSO shall have the authority, with the concurrence of Hazards Control, to institute any change to maintain health and safety protection for workers at Site 300.

Forsmark site investigation. Interpretation of topographic lineaments 2002

International Nuclear Information System (INIS)

Isaksson, Hans

2003-04-01

SKB performs site investigations for localization of a deep repository for high level radioactive waste. The site investigations are performed in two municipalities; Oesthammar and Oskarshamn. The Forsmark investigation area is situated in Oesthammar, close to the Forsmark nuclear power plant. The purpose of interpretation of lineaments from topographic data is to identify linear features (lineaments), which may correspond to deformation zones in the bedrock. The data will be combined with interpretations of lineaments from airborne geophysical data in order to produce an integrated lineament interpretation for the Forsmark area. This integrated interpretation will be combined with geological data in order to establish a bedrock geological map of the Forsmark area. The area for the lineament interpretation is the same as that selected for the bedrock mapping activities during 2002, i.e. the land area around Forsmark

Klipperaas study site. Scope of activities and main results

International Nuclear Information System (INIS)

Ahlbom, K.; Andersson, J.E.; Andersson, Peter; Ittner, T.; Tiren, S.; Ljunggren, C.

1992-09-01

During the period from 1977 - 1986 SKB (Swedish Nuclear Fuel and Waste Management Co.) performed surface and borehole investigations of 14 study sites for the purpose of assessing their suitability for a repository of spent nuclear fuel. The next phase in the SKB site selection rpogramme will be to perform detailed characterisation, including characterization from shafts and/or tunnels, of two or three sites. The detailed investigations will continue over several years to provide all the data needed for a licensing application to build a repository. Such an application is foreseen to be given to the authorities around the year 2003. It is presently not clear if any of the study sites will be selected as a site for detailed characterization. Other sites with geological and/or socio-economical characteristics judged more favorable may very well be the ones selected. However, as a part of the background documentation needed for the site selection studies to come, summary reports will be prepared for most study sites. These reports will include scope of activities, main results, uncertainties and need of complementary investigations. This report concern the Klipperaas study site. The main topics are the scope of activities, geologic model, geohydrological model, groundwater chemistry, assessment of solute transport, and rock mechanics

Salmon Site Remedial Investigation Report, Appendix B (Part 2)

International Nuclear Information System (INIS)

1999-01-01

This Salmon Site Remedial Investigation Report provides the results of activities initiated by the U.S. Department of Energy (DOE) to determine if contamination at the Salmon Site poses a current or future risk to human health and the environment. These results were used to develop and evaluate a range of risk-based remedial alternatives. Located in Lamar County, Mississippi, the Salmon Site was used by the U.S. Atomic Energy Commission (predecessor to the DOE) between 1964 and 1970 for two nuclear and two gas explosions conducted deep underground in a salt dome. The testing resulted in the release of radionuclides into the salt dome. During reentry drilling and other site activities, liquid and solid wastes containing radioactivity were generated resulting in surface soil and groundwater contamination. Most of the waste and contaminated soil and water were disposed of in 1993 during site restoration either in the cavities left by the tests or in an injection well. Other radioactive wastes were transported to the Nevada Test Site for disposal. Nonradioactive wastes were disposed of in pits at the site and capped with clean soil and graded. The preliminary investigation showed residual contamination in the Surface Ground Zero mud pits below the water table. Remedial investigations results concluded the contaminant concentrations detected present no significant risk to existing and/or future land users, if surface institutional controls and subsurface restrictions are maintained. Recent sampling results determined no significant contamination in the surface or shallow subsurface. The test cavity resulting from the experiments is contaminated and cannot be economically remediated with existing technologies. The ecological sampling did not detect biological uptake of contaminants in the plants or animals sampled. Based on the current use of the Salmon Site, the following remedial actions were identified to protect both human health and the environment: (1) the

Salmon Site Remedial Investigation Report, Appendix B (Part 1)

International Nuclear Information System (INIS)

1999-01-01

This Salmon Site Remedial Investigation Report provides the results of activities initiated by the U.S. Department of Energy (DOE) to determine if contamination at the Salmon Site poses a current or future risk to human health and the environment. These results were used to develop and evaluate a range of risk-based remedial alternatives. Located in Lamar County, Mississippi, the Salmon Site was used by the U.S. Atomic Energy Commission (predecessor to the DOE) between 1964 and 1970 for two nuclear and two gas explosions conducted deep underground in a salt dome. The testing resulted in the release of radionuclides into the salt dome. During reentry drilling and other site activities, liquid and solid wastes containing radioactivity were generated resulting in surface soil and groundwater contamination. Most of the waste and contaminated soil and water were disposed of in 1993 during site restoration either in the cavities left by the tests or in an injection well. Other radioactive wastes were transported to the Nevada Test Site for disposal. Nonradioactive wastes were disposed of in pits at the site and capped with clean soil and graded. The preliminary investigation showed residual contamination in the Surface Ground Zero mud pits below the water table. Remedial investigations results concluded the contaminant concentrations detected present no significant risk to existing and/or future land users, if surface institutional controls and subsurface restrictions are maintained. Recent sampling results determined no significant contamination in the surface or shallow subsurface. The test cavity resulting from the experiments is contaminated and cannot be economically remediated with existing technologies. The ecological sampling did not detect biological uptake of contaminants in the plants or animals sampled. Based on the current use of the Salmon Site, the following remedial actions were identified to protect both human health and the environment: (1) the

Energy transfer at the active sites of heme proteins

International Nuclear Information System (INIS)

Dlott, D.D.; Hill, J.R.

1995-01-01

Experiments using a picosecond pump-probe apparatus at the Picosecond Free-electron Laser Center at Stanford University, were performed to investigate the relaxation of carbon monoxide bound to the active sites of heme proteins. The significance of these experiments is two-fold: (1) they provide detailed information about molecular dynamics occurring at the active sites of proteins; and (2) they provide insight into the nature of vibrational relaxation processes in condensed matter. Molecular engineering is used to construct various molecular systems which are studied with the FEL. We have studied native proteins, mainly myoglobin obtained from different species, mutant proteins produced by genetic engineering using recombinant DNA techniques, and a variety of model systems which mimic the structures of the active sites of native proteins, which are produced using molecular synthesis. Use of these different systems permits us to investigate how specific molecular structural changes affect dynamical processes occurring at the active sites. This research provides insight into the problems of how different species needs are fulfilled by heme proteins which have greatly different functionality, which is induced by rather small structural changes

International workshop on site investigation and evaluation based on the siting process in Sweden

International Nuclear Information System (INIS)

Andersson, Johan; Stroem, A.

2001-06-01

SKB's goal is to commence surface based site investigations in 2002. Extensive preparations are now being made for this transition to the next phase in the siting process for the deep repository for spent nuclear fuel. The purpose of the international workshop on site characterisation held at Aespoe April 2001 was to: present the SKB site investigation and evaluation programme to a group of international experts; discuss whether the available toolbox of investigation methods for surface based site investigations is appropriate and state-of-the-art in an international perspective; and by working group sessions discuss the level of ambition in the programme for site investigation for each discipline. This report summarises the conclusions of the workshop in general terms. Many of the detailed comments and ideas obtained at the workshop have already inspired and will also directly inspire the on-going planning work for site characterisation. The core activity at the workshop was the work performed by working groups. They addressed what should be considered for a site characterisation programme, based on the generic planning made so far by SKB. The working groups also outlined site specific characterisation programmes for the sites suggested by SKB. The tasks were strictly confined to technical and scientific modelling issues. The working group chairmen presented the working group results at the workshop and have also submitted short memos to SKB. The present document is a compilation of these memos. The SKB generic programme as presented in the existing top level documents, 'Requirements and Criteria and Overall Programme', received general endorsement and was appreciated for being comprehensive and systematic. For example, it contains comprehensive lists of parameters to be measured. However, there is need for prioritisation and sequencing. This is actually included in the current planning process at SKB where the generic programme later this year will be adapted to

Active site - a site of binding of affinity inhibitors in baker's yeast inorganic pyrophosphatase

International Nuclear Information System (INIS)

Svyato, I.E.; Sklyankina, V.A.; Avaeva, S.M.

1986-01-01

The interaction of the enzyme-substrate complex with methyl phosphate, O-phosphoethanolamine, O-phosphopropanolamine, N-acetylphosphoserine, and phosphoglyolic acid, as well as pyrophosphatase, modified by monoesters of phosphoric acid, with pyrophosphate and tripolyphosphate, was investigated. It was shown that the enzyme containing the substrate in the active site does not react with monophosphates, but modified pyrophosphatase entirely retains the ability to bind polyanions to the regulatory site. It is concluded that the inactivation of baker's yeast inorganic pyrophosphatase by monoesters of phosphoric acid, which are affinity inhibitors of it, is the result of modification of the active site of the enzyme

Corrective Action Investigation Plan for Corrective Action Unit 554: Area 23 Release Site, Nevada Test Site, Nevada

International Nuclear Information System (INIS)

Boehlecke, Robert F.

2004-01-01

This Corrective Action Investigation Plan (CAIP) contains project-specific information for conducting site investigation activities at Corrective Action Unit (CAU) 554: Area 23 Release Site, Nevada Test Site, Nevada. Information presented in this CAIP includes facility descriptions, environmental sample collection objectives, and criteria for the selection and evaluation of environmental samples. Corrective Action Unit 554 is located in Area 23 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 554 is comprised of one Corrective Action Site (CAS), which is: 23-02-08, USTs 23-115-1, 2, 3/Spill 530-90-002. This site consists of soil contamination resulting from a fuel release from underground storage tanks (USTs). Corrective Action Site 23-02-08 is being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation prior to evaluating corrective action alternatives and selecting the appropriate corrective action for this CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document for CAU 554. Corrective Action Site 23-02-08 will be investigated based on the data quality objectives (DQOs) developed on July 15, 2004, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; and contractor personnel. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 554. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to CAS 23-02-08. The scope of the corrective action investigation

Execution programme for the initial site investigations at Forsmark

Energy Technology Data Exchange (ETDEWEB)

NONE

2002-05-01

In the feasibility studies that were completed in 2001, eight sites were identified as potentially suitable for hosting a repository. All the identified sites meet the safety requirements with respect to bedrock conditions that could be checked at that time. The feasibility studies have revealed good potential when it comes to the technical and environmental aspects as well. Based on an integrated evaluation SKB proposed to start site investigations with test drillings at three sites; Forsmark, Simpevarp and Tierp. Site investigations have started at Forsmark and Simpevarp. The municipal council of Tierp voted no to a site investigation in April 2002. The site investigations are divided into two main phases; initial and complete investigations. Initial site investigations are performed to identify the site within a specified area that is deemed to be most suitable for a deep repository and to determine whether the feasibility study's judgement of the suitability of the area holds up in the light of borehole data from repository depth. The initial site investigations are expected to take 1.5-2 years. If the assessment shows that the site has good potential to host a repository, complete site investigations will follow for an expected duration of 3.5-4 years. The purpose of the complete site investigations is to gather all information required to select one of the sites as the main alternative and to apply for a permit for construction of the deep repository at that site. A general programme in which the results from feasibility studies are summarized, the candidate sites presented and the framework of programme for the site investigation phase presented has been published. The general programme, and main references to the programme, specifies which data are required in order to design the repository and carry out a safety assessment, how the investigations should be carried out in order to provide these data, criteria with which the site must comply, as well as

Execution programme for the initial site investigations at Forsmark

International Nuclear Information System (INIS)

2002-05-01

In the feasibility studies that were completed in 2001, eight sites were identified as potentially suitable for hosting a repository. All the identified sites meet the safety requirements with respect to bedrock conditions that could be checked at that time. The feasibility studies have revealed good potential when it comes to the technical and environmental aspects as well. Based on an integrated evaluation SKB proposed to start site investigations with test drillings at three sites; Forsmark, Simpevarp and Tierp. Site investigations have started at Forsmark and Simpevarp. The municipal council of Tierp voted no to a site investigation in April 2002. The site investigations are divided into two main phases; initial and complete investigations. Initial site investigations are performed to identify the site within a specified area that is deemed to be most suitable for a deep repository and to determine whether the feasibility study's judgement of the suitability of the area holds up in the light of borehole data from repository depth. The initial site investigations are expected to take 1.5-2 years. If the assessment shows that the site has good potential to host a repository, complete site investigations will follow for an expected duration of 3.5-4 years. The purpose of the complete site investigations is to gather all information required to select one of the sites as the main alternative and to apply for a permit for construction of the deep repository at that site. A general programme in which the results from feasibility studies are summarized, the candidate sites presented and the framework of programme for the site investigation phase presented has been published. The general programme, and main references to the programme, specifies which data are required in order to design the repository and carry out a safety assessment, how the investigations should be carried out in order to provide these data, criteria with which the site must comply, as well as

On-site and off-site activities

International Nuclear Information System (INIS)

Martin, H.D.

1986-01-01

Design principles for NPP training programs. Effects of NPP contracts. Effects of domestic industrial activities. The role of international bodies. Requirements for on-site training. Training abroad, technical, financial and social aspects. Training center on-site, an evaluation. (orig.)

Metal active site elasticity linked to activation of homocysteine in methionine synthases

Energy Technology Data Exchange (ETDEWEB)

Koutmos, Markos; Pejchal, Robert; Bomer, Theresa M.; Matthews, Rowena G.; Smith, Janet L.; Ludwig, Martha L. (Michigan)

2008-04-02

Enzymes possessing catalytic zinc centers perform a variety of fundamental processes in nature, including methyl transfer to thiols. Cobalamin-independent (MetE) and cobalamin-dependent (MetH) methionine synthases are two such enzyme families. Although they perform the same net reaction, transfer of a methyl group from methyltetrahydrofolate to homocysteine (Hcy) to form methionine, they display markedly different catalytic strategies, modular organization, and active site zinc centers. Here we report crystal structures of zinc-replete MetE and MetH, both in the presence and absence of Hcy. Structural investigation of the catalytic zinc sites of these two methyltransferases reveals an unexpected inversion of zinc geometry upon binding of Hcy and displacement of an endogenous ligand in both enzymes. In both cases a significant movement of the zinc relative to the protein scaffold accompanies inversion. These structures provide new information on the activation of thiols by zinc-containing enzymes and have led us to propose a paradigm for the mechanism of action of the catalytic zinc sites in these and related methyltransferases. Specifically, zinc is mobile in the active sites of MetE and MetH, and its dynamic nature helps facilitate the active site conformational changes necessary for thiol activation and methyl transfer.

Sternoe study site. Scope of activities and main results

International Nuclear Information System (INIS)

Ahlbom, K.; Andersson, J.E.; Nordqvist, R.; Tiren, S.; Ljunggren, C.; Voss, C.

1992-01-01

During the period from 1977-1986 SKB (Swedish Nuclear Fuel and Waste Management Co) performed surface and borehole investigations of 14 study sites for the purpose of assessing their suitability for a repository of spent nuclear fuel. The next phase in the SKB site selection programme will be to perform detailed characterization, including characterization from shafts and/or tunnels, of two or three sites. The detailed investigations will continue over several years to provide all the data needed for a licensing application to build a repository. Such an application is foreseen to be given to the authorities around the year 2003. It is presently not clear if anyone of the previously investigated study sites will be selected as a site for detailed characterization. Other sites with geological and/or socio-economical characteristics judged more favourable may very well be the ones selected. However, as a part of the background documentation needed for the site selection studies to come, summary reports will be prepared for most study sites. These reports will include scope of activities, main results, uncertainties and need of complementary investigations. This report concerns the Sternoe study site. This site was one of the first sites to be investigated by SKB . The studies at Sternoe were made under severe time-constraints and with prototype borehole instrumentations. These limitations should be kept in mind when reading the report. (41 refs., 16 figs., 12 tabs.) (au)

Half-of-the-sites reactivity of outer-membrane phospholipase A against an active-site-directed inhibitor.

Science.gov (United States)

Ubarretxena-Belandia, I; Cox, R C; Dijkman, R; Egmond, M R; Verheij, H M; Dekker, N

1999-03-01

The reaction of a novel active-site-directed phospholipase A1 inhibitor with the outer-membrane phospholipase A (OMPLA) was investigated. The inhibitor 1-p-nitrophenyl-octylphosphonate-2-tridecylcarbamoyl-3-et hanesulfonyl -amino-3-deoxy-sn-glycerol irreversibly inactivated OMPLA. The inhibition reaction did not require the cofactor calcium or an unprotonated active-site His142. The inhibition of the enzyme solubilized in hexadecylphosphocholine micelles was characterized by a rapid (t1/2 = 20 min) and complete loss of enzymatic activity, concurrent with the covalent modification of 50% of the active-site serines, as judged from the amount of p-nitrophenolate (PNP) released. Modification of the remaining 50% occurred at a much lower rate, indicative of half-of-the-sites reactivity against the inhibitor of this dimeric enzyme. Inhibition of monomeric OMPLA solubilized in hexadecyl-N,N-dimethyl-1-ammonio-3-propanesulfonate resulted in an equimolar monophasic release of PNP, concurrent with the loss of enzymatic activity (t1/2 = 14 min). The half-of-the-sites reactivity is discussed in view of the dimeric nature of this enzyme.

Gideaa study site. Scope of activities and main results

International Nuclear Information System (INIS)

Ahlbom, K.; Andersson, J.E.; Nordqvist, R.; Ljunggren, C.; Tiren, S.; Voss, C.

1991-10-01

During the period from 1977-1986 SKB (Swedish Nuclear Fuel and Waste Management Co) performed surface and borehole investigations of 14 study sites for the purpose of assessing their suitability for a repository of spent nuclear fuel. The next phase in the SKB site selection programme will be to perform detailed characterization, including characterization from shafts and/or tunnels, of two or three sites. The detailed investigations will continue over several years to provide all the data needed for a licensing application to build a repository. Such an application is foreseen to be given to the authorities around the year 2003. It is presently not clear if anyone of the study sites will be selected as a site for detailed characterization. Other site with geological and/or socio-economical characteristics judged more favourable may very well be the ones selected. However, as a part of the background documentation needed for the site selection studies to come, summary reports will be prepared for most study sites. These reports will include scope of activities, main results, uncertainties and need of complementary investigations. This report concerns the Gideaa study site. (au)

Kamlunge study site. Scope of activities and main results

International Nuclear Information System (INIS)

Ahlbom, K.; Andersson, J.E.; Andersson, P.; Ittner, T.; Tiren, S.; Ljunggren, C.

1992-05-01

During the period from 1977-1986 SKB (Swedish nuclear Fuel and Waste Management Co.) performed surface and borehole investigations of 14 study sites for the purpose of assessing their suitability for a repository of spent nuclear fuel. The next phase in the SKB site selection programme will be to perform detailed characterization, including characterization from shafts and/or tunnels, of two or three sites. The detailed investigations will continue over several years to provide all the data needed for a licensing application to build a repository. Such an application is foreseen to be given to the authorities around the year 2003. It is presently not clear if anyone of the study sites will be selected as a site for detailed characterization. Other sites with geological and/or socio-economical characteristics judged more favourable may very well be selected. However, as a part of the background documentation needed for the site selection studies to come, summary reports will be prepared for most study sites. These reports will include scope of activities, main results, uncertainties and need of complementary investigations. This report concerns the Kamlunge study site. (79 refs.) (au)

Fjaellveden study site. Scope of activities and main results

International Nuclear Information System (INIS)

Ahlbom, K.; Andersson, J.E.; Nordqvist, R.; Ljunggren, C.; Tiren, S.; Voss, C.

1991-10-01

During the period from 1977-1986 SKB (Swedish Nuclear Fuel and Waste Management CO) performed surface and borehole investigations of 14 study sites for the purpose of assessing their suitability for a repository of spent nuclear fuel. The next phase in the SKB site selection programme will be to perform detailed characterization, including characterization from shafts and/or tunnels, of two or three sites. The detailed investigations will continue over several years to provide all the data needed for a licensing application to build repository. Such an application is foreseen to be given to the authorities around the year 2003. It is presently not clear if anyone of the study sites will be selected as a site for detailed characterization. Other sites with geological and/or socio-economical characteristics judged more favourable may very well be the ones selected. However, as a part of the background documentation needed for the site selection studies to come, summary reports will be prepared for most study sites. These reports will include scope of activities, main results, uncertainties and need for complementary investigations. This report concerns the Fjaellveden study site. (au)

Summary report of the experiences from TVO's site investigations

International Nuclear Information System (INIS)

Oehberg, A.; Saksa, P.; Ahokas, H.; Ruotsalainen, P.; Snellman, M.

1994-05-01

Teollisuuden Voima Oy (TVO) has completed preliminary site investigations at five sites in Finland. At the end of 1992 TVO presented the final report to the authorities. The preliminary site investigation phase 1986-1992 was conducted according to the investigation programme compiled by TVO. The aim of this report was to compile a report on experiences from TVOs site investigations. The main interest was focused on investigation strategies and the most important investigation methods for the conceptual modelling. The objective of the preliminary site investigations was to obtain data on the bedrock properties in order to evaluate the areas. The programme was divided into four stages, each stage having its own sub-objective. The site-specific investigation programme for each site included a large common part and a small site-specific part. The strategies (objectives) and experiences from different disciplines, geology, hydrogeochemistry, geophysics and geohydrology, are presented in the report. The conceptual modelling work procedure including both bedrock and groundwater modelling is described briefly using the Olkiluoto site as an example. Each of the other areas has undergone similar phases of work. The uncertainties associated with conceptual modelling are also discussed. The usefulness of the investigation strategy and the investigation methods for conceptual modelling is discussed in the report. Some new equipment, methods or enhancements that have not yet been used in TVOs site investigations have become new tools in site characterisation and are briefly presented in the report. 52 refs, 35 figs, 1 tab

Hydrogeological Site Descriptive Model - a strategy for its development during Site Investigations

Energy Technology Data Exchange (ETDEWEB)

Rhen, Ingvar [SWECO VIAK AB, Goeteborg (Sweden); Follin, Sven [SF GeoLogic AB, Stockholm (Sweden); Hermanson, Jan [Golder Associates, Stockholm (Sweden)

2003-04-01

The report is to present a strategy for the development of the Site Descriptive Hydrogeological Model within the SKB Site Investigation Programme. The report, and similar reports from the Geology, Rock Mechanics, Thermal properties, Hydrogeochemistry, Transport Properties and Surface Ecosystem disciplines are intended to guide SKB Site Descriptive Modelling but also to provide the authorities with an overview of how the modelling should be performed. Thus the objectives of this report are to: provide guidelines for the modelling of different sites resulting in consistent handling of modelling issues during the Site Investigations, provide a structure for the modelling sequence that is suitable for the establishment of a Site Descriptive model and provide some necessary details that should be considered in a Site Descriptive model.

Hydrogeological Site Descriptive Model - a strategy for its development during Site Investigations

International Nuclear Information System (INIS)

Rhen, Ingvar; Follin, Sven; Hermanson, Jan

2003-04-01

The report is to present a strategy for the development of the Site Descriptive Hydrogeological Model within the SKB Site Investigation Programme. The report, and similar reports from the Geology, Rock Mechanics, Thermal properties, Hydrogeochemistry, Transport Properties and Surface Ecosystem disciplines are intended to guide SKB Site Descriptive Modelling but also to provide the authorities with an overview of how the modelling should be performed. Thus the objectives of this report are to: provide guidelines for the modelling of different sites resulting in consistent handling of modelling issues during the Site Investigations, provide a structure for the modelling sequence that is suitable for the establishment of a Site Descriptive model and provide some necessary details that should be considered in a Site Descriptive model

Execution programme for the initial site investigations at Simpevarp

Energy Technology Data Exchange (ETDEWEB)

NONE

2002-10-01

In the feasibility studies that were completed in 2001, eight sites were identified as potentially suitable for hosting a repository. All the identified sites meet the safety requirements with respect to bedrock conditions that could be checked at that time. The feasibility studies have revealed good potential when it comes to the technical and environmental aspects as well. Based on an integrated evaluation SKB proposed to start site investigations with test drillings at three sites; Simpevarp, Forsmark, and Tierp north. The site investigations have started at Simpevarp and Forsmark. The municipal council of Tierp rejected a site investigation in April 2002. The site investigations are divided into two main phases; initial and complete investigations. Initial Site Investigation is performed to identify the site within a specified area that is deemed to be most suitable for a deep repository and to determine whether the feasibility study's judgement of the suitability of the area holds up in the light of borehole data from repository depth. The Initial Site Investigation is expected to take about 3 years. If the assessment shows that the site has good potential to host a repository, Complete Site Investigation will follow for an expected duration of another 3 years. The purpose of the Complete Site Investigation is to gather all information required to select one of the sites as the main alternative and to apply for a permit for construction of the deep repository at that site. The site-specific programme gives an overview of the whole site investigation phase as well as a detailed description of the initial stage. The results of the initial investigations will determine whether Simpevarp is appropriate for further investigation, i.e. the Complete Site Investigation. This document summarizes the investigations that will be carried out at Simpevarp during the Initial Site Investigations. The document is a working document, which will be successively updated as

Execution programme for the initial site investigations at Simpevarp

International Nuclear Information System (INIS)

2002-10-01

In the feasibility studies that were completed in 2001, eight sites were identified as potentially suitable for hosting a repository. All the identified sites meet the safety requirements with respect to bedrock conditions that could be checked at that time. The feasibility studies have revealed good potential when it comes to the technical and environmental aspects as well. Based on an integrated evaluation SKB proposed to start site investigations with test drillings at three sites; Simpevarp, Forsmark, and Tierp north. The site investigations have started at Simpevarp and Forsmark. The municipal council of Tierp rejected a site investigation in April 2002. The site investigations are divided into two main phases; initial and complete investigations. Initial Site Investigation is performed to identify the site within a specified area that is deemed to be most suitable for a deep repository and to determine whether the feasibility study's judgement of the suitability of the area holds up in the light of borehole data from repository depth. The Initial Site Investigation is expected to take about 3 years. If the assessment shows that the site has good potential to host a repository, Complete Site Investigation will follow for an expected duration of another 3 years. The purpose of the Complete Site Investigation is to gather all information required to select one of the sites as the main alternative and to apply for a permit for construction of the deep repository at that site. The site-specific programme gives an overview of the whole site investigation phase as well as a detailed description of the initial stage. The results of the initial investigations will determine whether Simpevarp is appropriate for further investigation, i.e. the Complete Site Investigation. This document summarizes the investigations that will be carried out at Simpevarp during the Initial Site Investigations. The document is a working document, which will be successively updated as

Summary report of the experiences from TVO's site investigations

International Nuclear Information System (INIS)

Oehberg, A.; Saksa, P.; Ahokas, H.; Ruotsalainen, P.; Snellman, M.

1994-09-01

In 1992 Teollisuuden Voima Oy (TVO) completed preliminary site investigations for radioactive waste disposal at five sites in Finland. The aim of this report was the compilation of the experiences from TVO's site investigations. The main interest was focused on investigation strategies and the most important investigation methods for the conceptual modelling. The objective of the preliminary site investigations was to obtain data on the bedrock properties in order to evaluate the areas. The programme was divided into four stages, each stage having its own subobjective. The site-specific investigation programme for each site included a large common part and a small site-specific part. The strategies (objectives) and experiences from different disciplines, geology, hydrogeochemistry, geophysics and geohydrology, are presented in the report. The conceptual modelling work procedure including both bedrock and groundwater modelling is described briefly using the Olkiluoto site as an example. Each of the other areas has undergone similar phases of work. (52 refs., 45 figs., 5 tabs.)

Site Safety Plan for Lawrence Livermore National Laboratory CERCLA investigations

Energy Technology Data Exchange (ETDEWEB)

Bainer, R.; Duarte, J.

1993-07-01

The safety policy of LLNL is to take every reasonable precaution in the performance of work to protect the environment and the health and safety of employees and the public, and to prevent property damage. With respect to hazardous agents, this protection is provided by limiting human exposures, releases to the environment, and contamination of property to levels that are as low as reasonably achievable (ALARA). It is the intent of this Plan to supply the broad outline for completing environmental investigations within ALARA guidelines. It may not be possible to determine actual working conditions in advance of the work; therefore, planning must allow the opportunity to provide a range of protection based upon actual working conditions. Requirements will be the least restrictive possible for a given set of circumstances, such that work can be completed in an efficient and timely fashion. Due to the relatively large size of the LLNL Site and the different types of activities underway, site-specific Operational Safety Procedures (OSPs) will be prepared to supplement activities not covered by this Plan. These site-specific OSPs provide the detailed information for each specific activity and act as an addendum to this Plan, which provides the general plan for LLNL Main Site operation.

DOE site performance assessment activities

International Nuclear Information System (INIS)

1990-07-01

Information on performance assessment capabilities and activities was collected from eight DOE sites. All eight sites either currently dispose of low-level radioactive waste (LLW) or plan to dispose of LLW in the near future. A survey questionnaire was developed and sent to key individuals involved in DOE Order 5820.2A performance assessment activities at each site. The sites surveyed included: Hanford Site (Hanford), Idaho National Engineering Laboratory (INEL), Los Alamos National Laboratory (LANL), Nevada Test Site (NTS), Oak Ridge National Laboratory (ORNL), Paducah Gaseous Diffusion Plant (Paducah), Portsmouth Gaseous Diffusion Plant (Portsmouth), and Savannah River Site (SRS). The questionnaire addressed all aspects of the performance assessment process; from waste source term to dose conversion factors. This report presents the information developed from the site questionnaire and provides a comparison of site-specific performance assessment approaches, data needs, and ongoing and planned activities. All sites are engaged in completing the radioactive waste disposal facility performance assessment required by DOE Order 5820.2A. Each site has achieved various degrees of progress and have identified a set of critical needs. Within several areas, however, the sites identified common needs and questions

Review of Investigations on Site Selection for Nuclear Power Plants in Croatia

Energy Technology Data Exchange (ETDEWEB)

Malbasa, N [Ekonerg, Zagreb (Croatia)

2008-07-01

A review of site investigation for nuclear facilities in the Republic of Croatia that had been performed from 1964, when investigation started for the first nuclear power plant, to 1994 when the activities were stopped, is presented therein. Brief results of the main investigation were presented including the Tanja site on the Danube upstream of Vukovar. It is the best of all the investigated locations for nuclear power plant in Croatia. The review of results for site selection of low and intermediate level of radioactive waste disposal is also given. The position of nuclear power plants in the strategic documents of the Republic of Croatia was analysed. It is concluded that the status of nuclear facilities in the main strategic documents must be improved because the energy future in Croatia - as almost in all European countries - could hardly be successful without any further development of nuclear energy.(author)

Review of Investigations on Site Selection for Nuclear Power Plants in Croatia

International Nuclear Information System (INIS)

Malbasa, N.

2008-01-01

A review of site investigation for nuclear facilities in the Republic of Croatia that had been performed from 1964, when investigation started for the first nuclear power plant, to 1994 when the activities were stopped, is presented therein. Brief results of the main investigation were presented including the Tanja site on the Danube upstream of Vukovar. It is the best of all the investigated locations for nuclear power plant in Croatia. The review of results for site selection of low and intermediate level of radioactive waste disposal is also given. The position of nuclear power plants in the strategic documents of the Republic of Croatia was analysed. It is concluded that the status of nuclear facilities in the main strategic documents must be improved because the energy future in Croatia - as almost in all European countries - could hardly be successful without any further development of nuclear energy.(author)

Corrective Action Investigation Plan for Corrective Action Unit 137: Waste Disposal Sites, Nevada Test Site, Nevada, Rev. No.:0

Energy Technology Data Exchange (ETDEWEB)

Wickline, Alfred

2005-12-01

This Corrective Action Investigation Plan (CAIP) contains project-specific information including facility descriptions, environmental sample collection objectives, and criteria for conducting site investigation activities at Corrective Action Unit (CAU) 137: Waste Disposal Sites. This CAIP has been developed in accordance with the ''Federal Facility Agreement and Consent Order'' (FFACO) (1996) that was agreed to by the State of Nevada, the U.S. Department of Energy (DOE), and the U.S. Department of Defense. Corrective Action Unit 137 contains sites that are located in Areas 1, 3, 7, 9, and 12 of the Nevada Test Site (NTS), which is approximately 65 miles (mi) northwest of Las Vegas, Nevada (Figure 1-1). Corrective Action Unit 137 is comprised of the eight corrective action sites (CASs) shown on Figure 1-1 and listed below: (1) CAS 01-08-01, Waste Disposal Site; (2) CAS 03-23-01, Waste Disposal Site; (3) CAS 03-23-07, Radioactive Waste Disposal Site; (4) CAS 03-99-15, Waste Disposal Site; (5) CAS 07-23-02, Radioactive Waste Disposal Site; (6) CAS 09-23-07, Radioactive Waste Disposal Site; (7) CAS 12-08-01, Waste Disposal Site; and (8) CAS 12-23-07, Waste Disposal Site. The Corrective Action Investigation (CAI) will include field inspections, radiological surveys, geophysical surveys, sampling of environmental media, analysis of samples, and assessment of investigation results, where appropriate. Data will be obtained to support corrective action alternative evaluations and waste management decisions. The CASs in CAU 137 are being investigated because hazardous and/or radioactive constituents may be present in concentrations that could potentially pose a threat to human health and the environment. Existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives for the CASs. Additional information will be generated by conducting a CAI before evaluating and selecting

Site description of Forsmark at completion of the site investigation phase. SDM-Site Forsmark

Energy Technology Data Exchange (ETDEWEB)

2008-12-15

The Swedish Nuclear Fuel and Waste Management Co., SKB, has undertaken site characterisation in two different areas, Forsmark and Laxemar-Simpevarp, in order to identify a suitable location for a geological repository of spent nuclear fuel according to the KBS-3 method. The site investigations have been conducted in campaigns, punctuated by data freezes. After each data freeze, the site data have been analysed and modelling has been carried out with the overall purpose to develop a site descriptive model (SDM). The site descriptive model is used by repository engineering to design the underground facility and to develop a repository layout adapted to the site. It is also essential for safety assessment, since the model is the only source for site-specific input. Another important use of the site descriptive model is in the environmental impact assessment. An SDM is an integrated model for geology, thermal properties, rock mechanics, hydrogeology, hydrogeochemistry, bedrock transport properties and a description of the surface system. The site descriptive model compiled in the current report, SDM-Site, presents an integrated understanding of the Forsmark area at the completion of the surface-based investigations, which were conducted at Forsmark during the period 2002 to 2007. It also provides a summary of the abundant underlying data and the discipline-specific models that support the site understanding. The description relies heavily on background reports that address, in particular, details in data analyses and modelling in the different disciplines. The Forsmark area is located in northern Uppland within the municipality of Oesthammar, about 120 km north of Stockholm. The candidate area for site investigation is located along the shoreline of Oeregrundsgrepen, within the north-western part of a major tectonic lens that formed between 1.87 and 1.85 billion years ago during the Svecokarelian orogeny. The candidate area is approximately 6 km long and 2 km wide. The

Site description of Laxemar at completion of the site investigation phase. SDM-Site Laxemar

Energy Technology Data Exchange (ETDEWEB)

2009-12-15

The Swedish Nuclear Fuel and Waste Management Company (SKB) has undertaken site characterisation in two different areas, Forsmark and Laxemar-Simpevarp, in order to identify a suitable location for a geological repository of spent nuclear fuel according to the KBS-3 method. The site investigations have been conducted in campaigns, punctuated by data freezes. After each data freeze, the site data have been analysed and modelling has been carried out with the overall purpose to develop a site descriptive model (SDM). The site descriptive model is used by repository engineering to design the underground facility and to develop a repository layout adapted to the site. It is also essential for safety assessment, since the SDM is the only source for site-specific input. Another important use of the site descriptive model is in the environmental impact assessment. An SDM is an integrated model of geology, thermal properties, rock mechanics, hydrogeology, hydrogeochemistry, bedrock transport properties and a description of the surface system. The site descriptive model compiled in the current report, SDM-Site Laxemar, presents an integrated understanding of the Laxemar-Simpevarp area (with special emphasis on the Laxemar subarea) at the completion of the surface-based investigations, which were conducted during the period 2002 to 2007. A summary is also provided of the abundant underlying data and the discipline specific models that support the site understanding. The description relies heavily on background reports that address, in particular, details of the data analyses and modelling of the different disciplines. The Laxemar-Simpevarp area is located in the province of Smaaland within the municipality of Oskarshamn, about 230 km south of Stockholm. The candidate area for site investigation is located along the shoreline of the strait of Kalmarsund, within a 1.8 billion year old suite of well preserved bedrock belonging to the Transscandinavian Igneous Belt formed during

Site description of Laxemar at completion of the site investigation phase. SDM-Site Laxemar

International Nuclear Information System (INIS)

2009-12-01

The Swedish Nuclear Fuel and Waste Management Company (SKB) has undertaken site characterisation in two different areas, Forsmark and Laxemar-Simpevarp, in order to identify a suitable location for a geological repository of spent nuclear fuel according to the KBS-3 method. The site investigations have been conducted in campaigns, punctuated by data freezes. After each data freeze, the site data have been analysed and modelling has been carried out with the overall purpose to develop a site descriptive model (SDM). The site descriptive model is used by repository engineering to design the underground facility and to develop a repository layout adapted to the site. It is also essential for safety assessment, since the SDM is the only source for site-specific input. Another important use of the site descriptive model is in the environmental impact assessment. An SDM is an integrated model of geology, thermal properties, rock mechanics, hydrogeology, hydrogeochemistry, bedrock transport properties and a description of the surface system. The site descriptive model compiled in the current report, SDM-Site Laxemar, presents an integrated understanding of the Laxemar-Simpevarp area (with special emphasis on the Laxemar subarea) at the completion of the surface-based investigations, which were conducted during the period 2002 to 2007. A summary is also provided of the abundant underlying data and the discipline specific models that support the site understanding. The description relies heavily on background reports that address, in particular, details of the data analyses and modelling of the different disciplines. The Laxemar-Simpevarp area is located in the province of Smaaland within the municipality of Oskarshamn, about 230 km south of Stockholm. The candidate area for site investigation is located along the shoreline of the strait of Kalmarsund, within a 1.8 billion year old suite of well preserved bedrock belonging to the Transscandinavian Igneous Belt formed during

Site description of Forsmark at completion of the site investigation phase. SDM-Site Forsmark

International Nuclear Information System (INIS)

2008-12-01

The Swedish Nuclear Fuel and Waste Management Co., SKB, has undertaken site characterisation in two different areas, Forsmark and Laxemar-Simpevarp, in order to identify a suitable location for a geological repository of spent nuclear fuel according to the KBS-3 method. The site investigations have been conducted in campaigns, punctuated by data freezes. After each data freeze, the site data have been analysed and modelling has been carried out with the overall purpose to develop a site descriptive model (SDM). The site descriptive model is used by repository engineering to design the underground facility and to develop a repository layout adapted to the site. It is also essential for safety assessment, since the model is the only source for site-specific input. Another important use of the site descriptive model is in the environmental impact assessment. An SDM is an integrated model for geology, thermal properties, rock mechanics, hydrogeology, hydrogeochemistry, bedrock transport properties and a description of the surface system. The site descriptive model compiled in the current report, SDM-Site, presents an integrated understanding of the Forsmark area at the completion of the surface-based investigations, which were conducted at Forsmark during the period 2002 to 2007. It also provides a summary of the abundant underlying data and the discipline-specific models that support the site understanding. The description relies heavily on background reports that address, in particular, details in data analyses and modelling in the different disciplines. The Forsmark area is located in northern Uppland within the municipality of Oesthammar, about 120 km north of Stockholm. The candidate area for site investigation is located along the shoreline of Oeregrundsgrepen, within the north-western part of a major tectonic lens that formed between 1.87 and 1.85 billion years ago during the Svecokarelian orogeny. The candidate area is approximately 6 km long and 2 km wide. The

Hydrogeochemical site descriptive model - a strategy for the model development during site investigations

Energy Technology Data Exchange (ETDEWEB)

Smellie, John [Conterra AB, Uppsala (Sweden); Laaksoharju, Marcus [GeoPoint AB, Sollentuna (Sweden); Tullborg, Eva-Lena [Terralogica AB, Graabo (Sweden)

2002-12-01

detrimentally affect the overall aim of providing sufficient and reliable scientific knowledge to describe adequately and accurately the hydrogeochemical conditions at the site. In addition to these constraints, certain priorities have to be listed and addressed because of the size and complexity of the site investigation programme. Such priorities may be planned prior to field activities, whilst others will result from an on-going evaluation of field activities and scientific or modelling needs under the direction of the SKB Site Analysis Team. This team will liaise closely with those responsible field personnel. Based on past experience, identified priorities include: minimising groundwater contamination in the collected samples; sampling end member groundwater conditions (e.g. recharge/discharge conditions, or high/low topographic locations and shorelines) to define important boundary conditions for hydrogeochemical and hydrodynamic modelling; sampling of end member water compositions rather than groundwater mixtures; sampling groundwaters close to repository depth; understanding the spatial variation of chemical parameters closely integrated with hydrogeological conditions; measuring a large number of spatially strategic points (including time series measurements) distributed vertically and laterally over a large area; simultaneous sampling from many boreholes to produce accurate, time-related 3D interpolated snap shots of important groundwater parameters. Sampling of low permeable rocks and fracture zones of low transmissivities will be allocated greater priority. Some five classes of chemical analyses are available to characterise the collected groundwater samples. The choice of class will depend on the hydrogeochemical information required, the location to be sampled and the sampling method used. The major hydrogeochemical output data from a site characterisation will refer mainly to chemistry, isotopes, dissolved gas contents, redox potential conditions, presence of

Corrective Action Investigation Plan for Corrective Action Unit 571: Area 9 Yucca Flat Plutonium Dispersion Sites, Nevada National Security Site, Nevada, Revision 0

Energy Technology Data Exchange (ETDEWEB)

Bailey, Bernadine; Matthews, Patrick

2013-07-01

CAU 571 is a grouping of sites where there has been a suspected release of contamination associated with nuclear testing. This document describes the planned investigation of CAU 571, which comprises the following corrective action sites (CASs): • 09-23-03, Atmospheric Test Site S-9F • 09-23-04, Atmospheric Test Site T9-C • 09-23-12, Atmospheric Test Site S-9E • 09-23-13, Atmospheric Test Site T-9D • 09-45-01, Windrows Crater These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the investigation report. The sites will be investigated based on the data quality objectives (DQOs) developed on March 6, 2013, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office (now the Nevada Field Office). The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 571. The site investigation process will also be conducted in accordance with the Soils Activity Quality Assurance Plan, which establishes requirements, technical planning, and general quality practices to be applied to this activity. The potential contamination sources associated with CAU 571 CASs are from nuclear testing activities. The DQO process resulted in an assumption that total effective dose (TED) within a default contamination boundary exceeds the final action level (FAL) and requires corrective action. The presence and nature of contamination outside the default

Role of active site rigidity in activity: MD simulation and fluorescence study on a lipase mutant.

Directory of Open Access Journals (Sweden)

Md Zahid Kamal

Full Text Available Relationship between stability and activity of enzymes is maintained by underlying conformational flexibility. In thermophilic enzymes, a decrease in flexibility causes low enzyme activity while in less stable proteins such as mesophiles and psychrophiles, an increase in flexibility is associated with enhanced enzyme activity. Recently, we identified a mutant of a lipase whose stability and activity were enhanced simultaneously. In this work, we probed the conformational dynamics of the mutant and the wild type lipase, particularly flexibility of their active site using molecular dynamic simulations and time-resolved fluorescence techniques. In contrast to the earlier observations, our data show that active site of the mutant is more rigid than wild type enzyme. Further investigation suggests that this lipase needs minimal reorganization/flexibility of active site residues during its catalytic cycle. Molecular dynamic simulations suggest that catalytically competent active site geometry of the mutant is relatively more preserved than wild type lipase, which might have led to its higher enzyme activity. Our study implies that widely accepted positive correlation between conformation flexibility and enzyme activity need not be stringent and draws attention to the possibility that high enzyme activity can still be accomplished in a rigid active site and stable protein structures. This finding has a significant implication towards better understanding of involvement of dynamic motions in enzyme catalysis and enzyme engineering through mutations in active site.

Geochemical investigation of UMTRAP designated site at Grand Junction, Colorado

International Nuclear Information System (INIS)

Markos, G.; Bush, K.J.

1983-09-01

This report is the result of a geochemical investigation of the former uranium mill and tailings site at Grand Junction, Colorado. The objectives of the investigation are to characterize the geochemistry, to determine the contaminant distribution resulting from the former milling activities and tailings, and to infer chemical pathways and transport mechanisms from the contaminant distribution. The results should be used to model contaminant migration and to develop criteria for long-term containment media, such as a cover system which is impermeable to contaminant migration. This report assumes a familiarity with the hydrologic conditions of the site and the geochemical concepts underlying the investigation. The results reported are based on a sampling of waters in two seasons and solid material from the background, the area adjacent to the site, and the site. The solid samples were water extracted to remove easily soluble salts and acid extracted to remove carbonates and hydroxides. The water extracts and solid samples were analyzed for the major and trace elements. A limited number of samples were analyzed for radiological components. The report includes the methods of sampling, sample processing, analysis, and data interpretation. Four major conclusions are: (1) trace element concentrations in shallow subsurface waters adjacent to the tailings temporally vary up to an order of magnitude; (2) the riverbank soils and borehole waters are contaminated with uranium, radium, and trace elements from discharge of tailings solids and solutions during the active time of the mill; however, the movement of contaminants toward the Colorado River does not appear to be significant; (3) the Colorado River adjacent to the tailings is not contaminated; and (4) trace metals have accumulated at both the tailings/cover and tailings/soil interface because of precipitation reactions caused by chemical differences between the two materials

Oskarshamn site investigation. Programme for further investigations of bedrock, soil, water and environment in Laxemar subarea

International Nuclear Information System (INIS)

2006-03-01

SKB (the Swedish Nuclear Fuel and Waste Management Co), has been conducting a site investigation at Simpevarp and Laxemar in Oskarshamn for siting of a final repository for spent nuclear fuel. An equivalent investigation is being conducted in Forsmark in Ohmmeter's. The initial part of the site investigations had been completed for the both of the subareas Simpevarp and Laxemar in the autumn of 2004. Based on the results of these investigations, SKB preliminarily prioritized the Laxemar subarea for further investigations. A programme was presented for the first stage of the complete site investigation in the Laxemar subarea, along with the main features of the remainder of the site investigation. The programme included investigations up until the summer of 2005 and was particularly aimed at obtaining answers to several vital questions so that the subsequent investigations could be focused on the rock areas judged to be most suitable for a final repository. These investigations have now been completed. This report presents the programme for the remainder of the site investigation. The points of departure are the general goals for the Deep Repository Project during the site investigation phase, analyses and evaluations of data from completed investigations, and the needs for additional data to be able to evaluate the site as a siting alternative for the final repository. The account mainly covers the investigations on the site. All other work - analyses, site descriptive modelling, facility design, safety assessments and studies and assessments of consequences for the environment, human health and society - are only mentioned to the extent necessary in order to place the investigations in their context. The direction of the site investigation in Oskarshamn and the investigation programme presented in this report is based on SKB's preliminary decision to prioritize the Laxemar subarea for further investigations. A final decision on the direction of the site

Alternative approaches to improve site investigations

International Nuclear Information System (INIS)

Beach, R.B.; Silka, L.R.

1992-01-01

Common complaints about standard investigations at hazardous waste sites include high costs and long time frames. Investigations at military bases as part of the installation restoration program or base closures suffer additionally from nonuniformity of approach and results and redundancy of work effort conducted by multiple environmental contractors. The problems of high costs and long time frames can be minimized by the consistent use of alternative sampling methods (such as soil gas surveys) and the utilization of analytical screening procedures at both on-site and off-site laboratories. Acceptable data quality is maintained by several procedures. Incorporation of quality control measures (10 % frequency), such as matrix spikes and duplicates, into the alternative analytical techniques allows assessment of the data quality relative to predetermined data quality objectives (DQOs). Confirmation of the screening results (10% frequency) using standard US EPA methods, such as the contract laboratory program (CLP) statement of work (SOW), allows an additional evaluation of the data accuracy. Depending on the investigative objectives, knowledge based computer systems (expert systems,) could be used to improve uniformity of site evaluations. Several case histories will be presented demonstrating how soil gas surveys, screening analyses and standard analyses can be utilized to give increased site information in a reduced time frame and at a cost savings of 30 to 40%. One case history illustrates a screening technique developed by the author for polynuclear aromatics (semi-volatile organic compounds) that can be conducted at a cost savings of 90% relative to a standard US EPA method. A comparison of the phased investigative approach to one using an integrated field team is presented for fuel spill or UST areas

Oskarshamn site investigation. Programme for further investigations of bedrock, soil, water and environment in Laxemar subarea

Energy Technology Data Exchange (ETDEWEB)

NONE

2006-03-15

SKB (the Swedish Nuclear Fuel and Waste Management Co), has been conducting a site investigation at Simpevarp and Laxemar in Oskarshamn for siting of a final repository for spent nuclear fuel. An equivalent investigation is being conducted in Forsmark in Ohmmeter's. The initial part of the site investigations had been completed for the both of the subareas Simpevarp and Laxemar in the autumn of 2004. Based on the results of these investigations, SKB preliminarily prioritized the Laxemar subarea for further investigations. A programme was presented for the first stage of the complete site investigation in the Laxemar subarea, along with the main features of the remainder of the site investigation. The programme included investigations up until the summer of 2005 and was particularly aimed at obtaining answers to several vital questions so that the subsequent investigations could be focused on the rock areas judged to be most suitable for a final repository. These investigations have now been completed. This report presents the programme for the remainder of the site investigation. The points of departure are the general goals for the Deep Repository Project during the site investigation phase, analyses and evaluations of data from completed investigations, and the needs for additional data to be able to evaluate the site as a siting alternative for the final repository. The account mainly covers the investigations on the site. All other work - analyses, site descriptive modelling, facility design, safety assessments and studies and assessments of consequences for the environment, human health and society - are only mentioned to the extent necessary in order to place the investigations in their context. The direction of the site investigation in Oskarshamn and the investigation programme presented in this report is based on SKB's preliminary decision to prioritize the Laxemar subarea for further investigations. A final decision on the direction of the site

Geotechnical investigations of the PEP site

International Nuclear Information System (INIS)

Gould, R.S.

1976-02-01

The purpose of this paper is to summarize the general nature of the geology and rock and soil formations of the PEP site as they relate to the design and construction of the project; to describe site investigation programs and to catalog the geotechnical information presently available about the site. The recently-completed investigation of subterranean conditions around the PEP ring when coupled with previous surveys gives us a good understanding of what to expect with regard to tunneling, undertaking larger underground excavations and constructing research halls are the interaction areas. It bears out the predictions made in Jacobs and Associates' report of 1973; i.e., that the ring housing construction is classified as soft-ground tunneling and that large underground openings, such as region 10 and the injection junction structures, will require great attention to support. A shield or shields will probably be required. On the positive side, the site affords very good conditions for soft-ground tunneling. Water will be a problem in some areas, but not an unsolvable one. The possibility of encountering lethal or explosive gases, almost always the case in tunneling in California's coastal formations, exists but has not been ascertained. Finally, no reasons to change current cost estimates or schedules have merged from the investigation. 13 refs., 1 fig

Corrective Action Investigation Plan for Corrective Action Unit 105: Area 2 Yucca Flat Atmospheric Test Sites Nevada National Security Site, Nevada, Revision 0

Energy Technology Data Exchange (ETDEWEB)

Patrick Matthews

2012-09-01

Corrective Action Unit (CAU) 105 is located in Area 2 of the Nevada National Security Site, which is approximately 65 miles northwest of Las Vegas, Nevada. CAU 105 is a geographical grouping of sites where there has been a suspected release of contamination associated with atmospheric nuclear testing. This document describes the planned investigation of CAU 105, which comprises the following corrective action sites (CASs): • 02-23-04, Atmospheric Test Site - Whitney • 02-23-05, Atmospheric Test Site T-2A • 02-23-06, Atmospheric Test Site T-2B • 02-23-08, Atmospheric Test Site T-2 • 02-23-09, Atmospheric Test Site - Turk These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on April 30, 2012, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 105. The site investigation process will also be conducted in accordance with the Soils Activity Quality Assurance Plan, which establishes requirements, technical planning, and general quality practices to be applied to this activity. The potential contamination sources associated with all CAU 105 CASs are from atmospheric nuclear testing activities. The presence and nature of contamination at CAU

Site investigations: Strategy for rock mechanics site descriptive model

International Nuclear Information System (INIS)

Andersson, Johan; Christiansson, Rolf; Hudson, John

2002-05-01

As a part of the planning work for the Site Investigations, SKB has developed a Rock Mechanics Site Descriptive Modelling Strategy. Similar strategies are being developed for other disciplines. The objective of the strategy is that it should guide the practical implementation of evaluating site specific data during the Site Investigations. It is also understood that further development may be needed. This methodology enables the crystalline rock mass to be characterised in terms of the quality at different sites, for considering rock engineering constructability, and for providing the input to numerical models and performance assessment calculations. The model describes the initial stresses and the distribution of deformation and strength properties of the intact rock, of fractures and fracture zones, and of the rock mass. The rock mass mechanical properties are estimated by empirical relations and by numerical simulations. The methodology is based on estimation of mechanical properties using both empirical and heroretical/numerical approaches; and estimation of in situ rock stress using judgement and numerical modelling, including the influence of fracture zones. These approaches are initially used separately, and then combined to produce the required characterisation estimates. The methodology was evaluated with a Test Case at the Aespoe Hard Rock Laboratory in Sweden. The quality control aspects are an important feature of the methodology: these include Protocols to ensure the structure and coherence of the procedures used, regular meetings to enhance communication, feedback from internal and external reviewing, plus the recording of an audit trail of the development steps and decisions made. The strategy will be reviewed and, if required, updated as appropriate

Site investigations: Strategy for rock mechanics site descriptive model

Energy Technology Data Exchange (ETDEWEB)

Andersson, Johan [JA Streamflow AB, Aelvsjoe (Sweden); Christiansson, Rolf [Swedish Nuclear Fuel and Waste Management Co., Stockholm (Sweden); Hudson, John [Rock Engineering Consultants, Welwyn Garden City (United Kingdom)

2002-05-01

As a part of the planning work for the Site Investigations, SKB has developed a Rock Mechanics Site Descriptive Modelling Strategy. Similar strategies are being developed for other disciplines. The objective of the strategy is that it should guide the practical implementation of evaluating site specific data during the Site Investigations. It is also understood that further development may be needed. This methodology enables the crystalline rock mass to be characterised in terms of the quality at different sites, for considering rock engineering constructability, and for providing the input to numerical models and performance assessment calculations. The model describes the initial stresses and the distribution of deformation and strength properties of the intact rock, of fractures and fracture zones, and of the rock mass. The rock mass mechanical properties are estimated by empirical relations and by numerical simulations. The methodology is based on estimation of mechanical properties using both empirical and heroretical/numerical approaches; and estimation of in situ rock stress using judgement and numerical modelling, including the influence of fracture zones. These approaches are initially used separately, and then combined to produce the required characterisation estimates. The methodology was evaluated with a Test Case at the Aespoe Hard Rock Laboratory in Sweden. The quality control aspects are an important feature of the methodology: these include Protocols to ensure the structure and coherence of the procedures used, regular meetings to enhance communication, feedback from internal and external reviewing, plus the recording of an audit trail of the development steps and decisions made. The strategy will be reviewed and, if required, updated as appropriate.

Site investigations for repositories for solid radioactive wastes in deep continental geological formations

International Nuclear Information System (INIS)

1982-01-01

This report reviews the earth-science investigations and associated scientific studies that may be needed to select a repository site and confirm that its characteristics are such that it will provide a safe confinement for solidified high-level and alpha-bearing and certain other solid radioactive wastes. Site investigations, as used in this report, cover earth sciences and associated safety analyses. Other site-investigation activities are identified but not otherwise considered here. The repositories under consideration are those consisting of mined cavities in deep continental rocks for accepting wastes in the solid and packaged form. The term deep as used in this report is used solely to emphasize the distinction between the repositories discussed in this report and those for shallow-ground disposal. In general, depths under consideration here are greater than 200 metres. The term continental refers to those geological formations that occur either beneath present-day land masses and adjoining islands or beneath the shallow seas. One of the objectives of site investigations is to collect the site-specific data necessary for the different evaluations, such as modelling required to assess the long-term safety of an underground repository

Site investigations for repositories for solid radioactive wastes in deep continental geological formations

Energy Technology Data Exchange (ETDEWEB)

1982-01-01

This report reviews the earth-science investigations and associated scientific studies that may be needed to select a repository site and confirm that its characteristics are such that it will provide a safe confinement for solidified high-level and alpha-bearing and certain other solid radioactive wastes. Site investigations, as used in this report, cover earth sciences and associated safety analyses. Other site-investigation activities are identified but not otherwise considered here. The repositories under consideration are those consisting of mined cavities in deep continental rocks for accepting wastes in the solid and packaged form. The term deep as used in this report is used solely to emphasize the distinction between the repositories discussed in this report and those for shallow-ground disposal. In general, depths under consideration here are greater than 200 metres. The term continental refers to those geological formations that occur either beneath present-day land masses and adjoining islands or beneath the shallow seas. One of the objectives of site investigations is to collect the site-specific data necessary for the different evaluations, such as modelling required to assess the long-term safety of an underground repository.

Communication activities for NUMO's site selection process

International Nuclear Information System (INIS)

Takeuchi, Mitsuo; Okuyama, Shigeru; Kitayama, Kazumi; Kuba, Michiyoshi

2004-01-01

A siting program for geological disposal of high-level radioactive waste (HLW) in Japan has just started and is moving into a new stage of communication with the public. A final repository site will be selected via a stepwise process, as stipulated in the Specified Radioactive Waste Final Disposal Act promulgated in June 2000. Based on the Act, the site selection process of the Nuclear Waste Management Organization of Japan (NUMO, established in October 2000) will be carried out in the three steps: selection of Preliminary Investigation Areas (PIAs), selection of Detailed Investigation Areas (DIAs) and selection of the Repository Site. The Act also defines NUMO's responsibilities in terms of implementing the HLW disposal program in an open and transparent manner. NUMO fully understands the importance of public participation in its activities and is aiming to promote public involvement in the process of site selection based on a fundamental policy, which consists of 'adopting a stepwise approach', 'respecting the initiative of municipalities' and 'ensuring transparency in information disclosure'. This policy is clearly reflected in the adoption of an open solicitation approach for volunteer municipalities for Preliminary Investigation Areas (PIAs). NUMO made the official announcement of the start of its open solicitation program on 19 December 2002. This paper outlines how NUMO's activities are currently carried out with a view to encouraging municipalities to volunteer as PIAs and how public awareness of the safety of the HLW disposal is evaluated at this stage

Hydrogeochemical site descriptive model - a strategy for the model development during site investigations

International Nuclear Information System (INIS)

Smellie, John; Laaksoharju, Marcus; Tullborg, Eva-Lena

2002-12-01

the overall aim of providing sufficient and reliable scientific knowledge to describe adequately and accurately the hydrogeochemical conditions at the site. In addition to these constraints, certain priorities have to be listed and addressed because of the size and complexity of the site investigation programme. Such priorities may be planned prior to field activities, whilst others will result from an on-going evaluation of field activities and scientific or modelling needs under the direction of the SKB Site Analysis Team. This team will liaise closely with those responsible field personnel. Based on past experience, identified priorities include: minimising groundwater contamination in the collected samples; sampling end member groundwater conditions (e.g. recharge/discharge conditions, or high/low topographic locations and shorelines) to define important boundary conditions for hydrogeochemical and hydrodynamic modelling; sampling of end member water compositions rather than groundwater mixtures; sampling groundwaters close to repository depth; understanding the spatial variation of chemical parameters closely integrated with hydrogeological conditions; measuring a large number of spatially strategic points (including time series measurements) distributed vertically and laterally over a large area; simultaneous sampling from many boreholes to produce accurate, time-related 3D interpolated snap shots of important groundwater parameters. Sampling of low permeable rocks and fracture zones of low transmissivities will be allocated greater priority. Some five classes of chemical analyses are available to characterise the collected groundwater samples. The choice of class will depend on the hydrogeochemical information required, the location to be sampled and the sampling method used. The major hydrogeochemical output data from a site characterisation will refer mainly to chemistry, isotopes, dissolved gas contents, redox potential conditions, presence of organic

Corrective Action Investigation Plan for Corrective Action Unit 570: Area 9 Yucca Flat Atmospheric Test Sites Nevada National Security Site, Nevada, Revision 0

Energy Technology Data Exchange (ETDEWEB)

Patrick Matthews

2012-08-01

CAU 570 comprises the following six corrective action sites (CASs): • 02-23-07, Atmospheric Test Site - Tesla • 09-23-10, Atmospheric Test Site T-9 • 09-23-11, Atmospheric Test Site S-9G • 09-23-14, Atmospheric Test Site - Rushmore • 09-23-15, Eagle Contamination Area • 09-99-01, Atmospheric Test Site B-9A These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on April 30, 2012, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 570. The site investigation process will also be conducted in accordance with the Soils Activity Quality Assurance Plan, which establishes requirements, technical planning, and general quality practices to be applied to this activity. The presence and nature of contamination at CAU 570 will be evaluated based on information collected from a field investigation. Radiological contamination will be evaluated based on a comparison of the total effective dose at sample locations to the dose-based final action level. The total effective dose will be calculated as the total of separate estimates of internal and external dose. Results from the analysis of soil samples will be used to calculate internal radiological

Activities of the senspol and NICOLE network concerning field investigation at contaminated sites

Energy Technology Data Exchange (ETDEWEB)

Ree, C.C.D.F. von [GeoDelft, AB Delft (Netherlands); Alcock, S. [Cranfield Univ. of Silsoe, Bedfordshire (United Kingdom)

2003-07-01

At a European level several networks can be identified aimed at developments and exchange of knowledge relevant to sustainable management of the subsurface. Based on the common interests in the field of site characterisation and monitoring the SENSPOL and NICOLE networks have established links resulting in cooperation in several project-activities. In this presentation two projects will be addressed: - Seville technicalmeeting (Aznacollar mining site). - Bridging gaps between sensor developers and (end) users in a pragmatic approach (GAPS-project). The goal of the Seville technical meeting was to apply the latest sensing technologies at a site contaminated by metal mining activities, to properly evaluate the advances and limitations in the monitoring of contaminated sites for sustainable land management and to determine further steps to commercial exploitation. Some 17 different instruments have been brought including: - Electrochemical sensors using different forms of anodic stripping voltammetry and constant current chronopotentiometry combined with several types of screen printed electrodes. - An amperometric biosensor using screen printed electrodes, which measures toxicity by inhibition effects on urease and its sensitive to Hg(II), Ag(I), Cu(I) and to a lesser extent to Pb(II), Zn(II) and Cd(II) and a selectrochemical DNA biosensor measuring overall toxicity. - A luminescent bacterial sensors for measuring the bioavailable fraction of Cd, Pb, Zn, Cu, As, and Hg, and a bioluminescent fiber optic sensor for Hg and As. - Toxicity testing instruments (Checklight 'ToxScreen Multi-Shot Test, ToxAlert {sup registered} 100 and ToxAlert {sup registered} 10) - A fieldprobe for pH, EC, TDS measurement - A lead automatic analyser AQUAMET using a ion-selective electrode. - Pulse-neutron borehole device in which interaction of neutrons with the surrounding medium can be used to monitor changes. In a two-day field session on the mining site participants were provided

Site Investigation for Detection of KIJANG Reactor Core Center

Energy Technology Data Exchange (ETDEWEB)

Kwon, Tae-Hyun; Kim, Jun Yeon; Kim, Jeeyoung [KAERI, Daejeon (Korea, Republic of)

2015-05-15

It was planned for the end of March 2017 and extended to April 2018 according to the government budget adjustment. The KJRR project is intended for filling the self-sufficiency of RI demand including Mo-99, increasing the NTD capacity and developing technologies related to the research reactor. In project, site investigation is the first activity that defines seismologic and related geologic aspects of the site. Site investigation was carried out from Oct. 2012 to Jan. 2014 and this study is intended to describe detail procedures in locating the reactor core center. The location of the reactor core center was determined by collectively reviewing not only geological information but also information from architects engineering. EL 50m was selected as ground level by levering construction cost. Four recommended locations (R-1a - R-1d) are displayed for the reactor core center. R-1a was found optimal in consideration of medium rock contour, portion of medium rock covering reactor buildings, construction cost, physical protection and electrical resistivity. It is noted that engineering properties of the medium rock is TCR/RQD 100/53, elastic modulus 7,710 - 8,720MPa, permeability coefficient 2.92E-06cm/s, and S-wave velocity 1,380m/s, sound for foundations of reactor buildings.

Animal investigation program 1974 annual report: Nevada Test Site and vicinity

International Nuclear Information System (INIS)

Smith, D.D.; Giles, K.R.; Bernhardt, D.E.; Brown, K.R.

1977-06-01

Data are presented from the radioanalysis of tissues collected from cattle, deer, desert bighorn sheep, and other wildlife that resided on or near the Nevada Test Site during 1974. Routine activities and special investigations of the Animal Investigation Program are also discussed. Other than the naturally occurring potassium-40, gamma-emitting radionuclides were detected infrequently. For example, cesium-137 was found only in the muscle tissues from 3 of the 12 Nevada Test Site cattle sampled during 1974. Tritium concentrations in the tissues from most of the animals sampled are at background levels. Animals from the experimental farm tended to have slightly higher concentrations than those sampled at other locations on the Nevada Test Site. Strontium-90 levels in bones from deer, desert bighorn sheep, and cattle were slightly lower than those reported for the preceding year. A graph depicts the average levels found in the bones of the three species from 1956 through 1974

Remedial Investigation of Hanford Site Releases to the Columbia River - 13603

Energy Technology Data Exchange (ETDEWEB)

Lerch, J.A.; Hulstrom, L.C. [Washington Closure Hanford, LLC, Richland, Washington 99354 (United States); Sands, J.P. [U.S Department of Energy, Richland Operations Office, Richland, Washington 99352 (United States)

2013-07-01

In south-central Washington State, the Columbia River flows through the U.S. Department of Energy Hanford Site. A primary objective of the Hanford Site cleanup mission is protection of the Columbia River, through remediation of contaminated soil and groundwater that resulted from its weapons production mission. Within the Columbia River system, surface water, sediment, and biota samples related to potential Hanford Site hazardous substance releases have been collected since the start of Hanford operations. The impacts from release of Hanford Site radioactive substances to the Columbia River in areas upstream, within, and downstream of the Hanford Site boundary have been previously investigated as mandated by the U.S. Department of Energy requirements under the Atomic Energy Act. The Remedial Investigation Work Plan for Hanford Site Releases to the Columbia River [1] was issued in 2008 to initiate assessment of the impacts under the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 [2]. The work plan established a phased approach to characterize contaminants, assess current risks, and determine whether or not there is a need for any cleanup actions. Field investigation activities over a 120-mile stretch of the Columbia River began in October 2008 and were completed in 2010. Sampled media included surface water, pore water, surface and core sediment, island soil, and fish (carp, walleye, whitefish, sucker, small-mouth bass, and sturgeon). Information and sample results from the field investigation were used to characterize current conditions within the Columbia River and assess whether current conditions posed a risk to ecological or human receptors that would merit additional study or response actions under CERCLA. The human health and ecological risk assessments are documented in reports that were published in 2012 [3, 4]. Conclusions from the risk assessment reports are being summarized and integrated with remedial investigation

Geochemical investigation of UMTRAP designated site at Salt Lake City, Utah

International Nuclear Information System (INIS)

Markos, G.; Bush, K.J.

1983-09-01

This report is the result of a geochemical investigation of the former uranium mill and tailings site at Salt Lake City, Utah. This is one in a series of site specific geochemical investigations performed on the inactive uranium mill tailings included in the Uranium Mill Tailings Remedial Action Project. The objectives of the investigation are to characterize the geochemistry, to determine the contaminant distribution resulting from the former milling activities and tailings, and to infer chemical pathways and transport mechanisms from the contaminant distribution. The results will be used to model contaminant migration and to develop criteria for long-term containment media such as a cover system which is impermeable to contaminant migration. This report assumes a familiarity with the hydrologic conditions of the site and the geochemical concepts underlying the investigation. The results reported are based on a one-time sampling of waters and solid material from the background, the area adjacent to the site, and the site. The solid samples were water extracted to remove easily soluble salts and acid extracted to remove carbonates and hydroxides. The water extracts and solid samples were anlyzed for the major and trace elements. The report includes the methods of sampling, sample processing, analysis, and data interpretation. Four major conclusions are: (1) sediments in the ditches and creeks adjacent to the site contain tailings, however, the waters were generally not contaminated; (2) tailings are mixed with the soils within a meter below the tailings in some locations, however, water-soluble contaminants decrease to below background levels within 30 cm below the tailings; (3) there has not been significant acid seepage into the soils below the tailings; and (4) salt crusts on the tailings contain trace elements, with the elements that form chloride complexes having the greatest accumulation

Integrated account of method, site selection and programme prior to the site investigation phase

International Nuclear Information System (INIS)

2000-12-01

In order to dispose of the spent nuclear fuel in a safe manner, SKB plans to site a deep repository and an encapsulation plant with associated canister fabrication and transportation system. After an integrated evaluation of feasibility studies and other material, SKB will proceed with investigations of the rock and studies regarding establishment of the deep disposal system in the municipality of Oskarshamn or in Northern Uppland. The plans also include further study of the prospects for a deep repository in the municipality of Nykoeping. In the municipality of Oskarshamn, SKB plans further studies of a siting of the deep repository at Simpevarp. There SKB wants to initiate site investigations with test drilling. For the encapsulation plant, SKB wants to continue studying a siting at CLAB. In Northern Uppland, SKB plans to study two siting alternatives for the deep repository. One is Forsmark in the municipality of Oesthammar, where SKB wants to initiate a site investigation with test drilling. The other is Tierp north of Skutskaer, where SKB intends to start test drilling in an area north of Tierp. First, however, a suitable drilling area with possible transport solutions needs to be defined. This alternative requires the participation of the municipalities of both Tierp and Aelvkarleby. A siting of the encapsulation plant in Northern Uppland will also be studied. For the municipality of Nykoeping, SKB plans to conduct a new safety assessment for the Fjaellveden area, based on data from previous investigations as well as additional studies of how a deep repository could be arranged. SKB will thereby gather data from yet another geographic and geological region beyond those that are prioritized. No test drilling is planned in Nykoeping. The goal of the site investigation phase is to obtain all permits needed to build the planned facilities. It will take an estimated 7 - 8 years to assemble the requisite supporting material, carry out consultations, compile siting

Parameters of importance to determine during geoscientific site investigation

Energy Technology Data Exchange (ETDEWEB)

Andersson, Johan [QuantiSci AB (Sweden); Almen, K.E. [KEA GEO-Konsult AB (Sweden); Ericsson, Lars O.; Karlsson, Fred; Stroem, A. [Swedish Nuclear Fuel and Waste Management Co., Stockholm (Sweden); Fredriksson, Anders [ADG Grundteknik AB (Sweden); Stanfors, R. [Roy Stanfors Consulting AB (Sweden)

1998-06-01

This document identifies and describes geo-scientific parameters that are of importance in order to carry out performance and safety assessments of a deep repository for spent nuclear fuel, based on the information that can be obtained from a site investigation. The document also discusses data needs for planning and design of the rock works and for description of other environmental aspects. Evaluation of the different parameters is discussed in the document as well. The document was produced by a working group consisting of the authors and various SKB staff and consultants, and comprises a step in the planning of a geo-scientific investigation programme at the sites where site investigations will be conducted. The goals of the work presented in this report can be derived directly from SKBs ongoing RD and D Programme. The programme stipulates that a geo-scientific site investigation programme must be available before a site investigation begins. This programme is supposed to specify the goals, measurement methods and evaluation methodology, as well as the acceptance criteria against which the site is evaluated. It is pointed out that site evaluation is a collective term for an interactive process consisting of different parts 65 refs, 15 figs, 12 tabs

Near Regional and Site Investigations of the Temelin NPP Site

International Nuclear Information System (INIS)

Prachar, Ivan; Vacek, Jiri; Heralecky, Pavel

2011-01-01

The Temelin NPP is worldwide through heated discussion with nuclear energetic opposition. In addition this discussion goes beyond a border of the Czech Republic. On the other side, results of several international supervisions shown that Temelin NPP is fully comparable with the safest nuclear power plants in the world regarding its technical design and safety functions. This presentation deals with the near regional and site investigations of the Temelin NPP Site. It must be noted that although the Temelin site is situated in the area with low seismicity, item of seismicity is a basic argument against Temelin NPP and therefore a detail seismic hazard assessment was performed

Active site loop conformation regulates promiscuous activity in a lactonase from Geobacillus kaustophilus HTA426.

Directory of Open Access Journals (Sweden)

Yu Zhang

Full Text Available Enzyme promiscuity is a prerequisite for fast divergent evolution of biocatalysts. A phosphotriesterase-like lactonase (PLL from Geobacillus kaustophilus HTA426 (GkaP exhibits main lactonase and promiscuous phosphotriesterase activities. To understand its catalytic and evolutionary mechanisms, we investigated a "hot spot" in the active site by saturation mutagenesis as well as X-ray crystallographic analyses. We found that position 99 in the active site was involved in substrate discrimination. One mutant, Y99L, exhibited 11-fold improvement over wild-type in reactivity (kcat/Km toward the phosphotriesterase substrate ethyl-paraoxon, but showed 15-fold decrease toward the lactonase substrate δ-decanolactone, resulting in a 157-fold inversion of the substrate specificity. Structural analysis of Y99L revealed that the mutation causes a ∼6.6 Å outward shift of adjacent loop 7, which may cause increased flexibility of the active site and facilitate accommodation and/or catalysis of organophosphate substrate. This study provides for the PLL family an example of how the evolutionary route from promiscuity to specificity can derive from very few mutations, which promotes alteration in the conformational adjustment of the active site loops, in turn draws the capacity of substrate binding and activity.

Potential energy center site investigations

International Nuclear Information System (INIS)

Savage, W.F.

1977-01-01

Past studies by the AEC, NRC, NSF and others have indicated that energy centers have certain advantages over dispersed siting. There is the need, however, to investigate such areas as possible weather modifications due to major heat releases, possible changes in Federal/state/local laws and institutional arrangements to facilitate implementation of energy centers, and to assess methods of easing social and economic pressures on a surrounding community due to center construction. All of these areas are under study by ERDA, but there remains the major requirement for the study of a potential site to yield a true assessment of the energy center concept. In this regard the Division of Nuclear Research and Applications of ERDA is supporting studies by the Southern and Western Interstate Nuclear Boards to establish state and utility interest in the concept and to carry out screening studies of possible sites. After selection of a final site for center study , an analysis will be made of the center including technical areas such as heat dissipation methods, water resource management, transmission methods, construction methods and schedules, co-located fuel cycle facilities, possible mix of reactor types, etc. Additionally, studies of safeguards, the interaction of all effected entities in the siting, construction, licensing and regulation of a center, labor force considerations in terms of local impact, social and economic changes, and financing of a center will be conducted. It is estimated that the potential site study will require approximately two years

Corrective Action Investigation Plan for Corrective Action Unit 567: Miscellaneous Soil Sites, Nevada National Security Site, Nevada, with ROTC 1 Revision 0

Energy Technology Data Exchange (ETDEWEB)

Matthews, Patrick K.

2013-07-01

Corrective Action Unit (CAU) 567 is located in Areas 1, 3, 5, 20, and 25 of the Nevada National Security Site, which is approximately 65 miles northwest of Las Vegas, Nevada. CAU 567 is a grouping of sites where there has been a suspected release of contamination associated with nuclear testing. This document describes the planned investigation of CAU 567, which comprises the following corrective action sites (CASs): • 01-23-03, Atmospheric Test Site T-1 • 03-23-25, Seaweed E Contamination Area • 05-23-07, A5b RMA • 20-23-08, Colby Mud Spill • 25-23-23, J-11 Soil RMA These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the investigation report. The sites will be investigated based on the data quality objectives (DQOs) developed on May 6, 2013, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Field Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 567. The site investigation process will also be conducted in accordance with the Soils Activity Quality Assurance Plan, which establishes requirements, technical planning, and general quality practices to be applied to this activity. The potential contamination sources associated with CAU 567 releases are nuclear test operations and other NNSS operations. The DQO process resulted in an assumption that total effective dose (TED) within a default contamination boundary

Main phases of siting for nuclear power plants with review of required investigations

International Nuclear Information System (INIS)

Malbasa, N.

1984-01-01

The purpose of the article is a short description of the main phases in the process of siting for nuclear power plants as interpreted and applied by the Institut za elektroprivredu, Zagreb in the screening, comparison and evaluation of the sites for NPPs in the SR of Croatia. The scope and purpose, as well as review of required data and investigations for each particular phase are given. Common used methods for the comparison of sites are described and example of rejection criteria applicable for early phases of the siting is proposed. It is given a list of the most important activities which detailed analysis id indispensable for ending of the evaluation and getting a site permit from the regulatory body. A legal and regulatory basis for carrying out the siting process is also described. (author)

Bovine nucleus caudatus acetylcholinesterase: active site determination and investigation of a dimeric form obtained by selective proteolysis

Energy Technology Data Exchange (ETDEWEB)

Landauer, P.; Ruess, K.P.; Lieflaender, M.

1984-09-01

The number of catalytic subunits of purified bovine nucleus caudatus acetylcholinesterase (E.C. 3.1.1.7) has been determined by active site labelling with (3H)diisopropyl fluorophosphate ((3H)DFP). The 10.5 S, 16 S, and 20 S forms were estimated to contain two, four, and six active sites, respectively, per molecule. A 4.8 S form, which showed a weak amphiphile-dependent activity behavior, was obtained by selective proteolytic digestion with pronase. The inability of the purified 4.8 S form to aggregate after detergent removal, and the molecular mass in the range of 130-165 kD under nondenaturating conditions, indicate that this form is a dimeric form, lacking those hydrophobic regions responsible for aggregation.

MX Siting Investigation. Water Resources Program Industry Activity Inventory, Nevada-Utah.

Science.gov (United States)

1980-09-02

Agua Caliente Existing 150 gpm *One of five sites shown on Plate II may be the site of this project. There are also three additional sites outside the...SOURCE: Wells WATER RECIRCULATED: 80%, hopefully WATER QUALITY: POTABLE : STOCK AGRICULTURE OTHER ? OPERATION - REOPENED: Reopened NEW: WATER...year _________ TYPE OF BENEFICIAL USE: __ * ~~WATER SOURCE: ____-____ WATER RECIRCULATED: ___ _____ WATER QUALITY: POTABLE : STOCK ___AGRICULTURE

Geophysical investigation, Salmon Site, Lamar County, Mississippi

International Nuclear Information System (INIS)

1995-02-01

Geophysical surveys were conducted in 1992 and 1993 on 21 sites at the Salmon Site (SS) located in Lamar County, Mississippi. The studies are part of the Remedial Investigation/Feasibility Study (RI/FS) being conducted by IT Corporation for the U.S. Department of Energy (DOE). During the 1960s, two nuclear devices and two chemical tests were detonated 826 meters (in) (2710 feet [ft]) below the ground surface in the salt dome underlying the SS. These tests were part of the Vela Uniform Program conducted to improve the United States capability to detect, identify, and locate underground nuclear detonations. The RI/FS is being conducted to determine if any contamination is migrating from the underground shot cavity in the salt dome and if there is any residual contamination in the near surface mud and debris disposal pits used during the testing activities. The objective of the surface geophysical surveys was to locate buried debris, disposal pits, and abandoned mud pits that may be present at the site. This information will then be used to identify the locations for test pits, cone penetrometer tests, and drill hole/monitor well installation. The disposal pits were used during the operation of the test site in the 1960s. Vertical magnetic gradient (magnetic gradient), electromagnetic (EM) conductivity, and ground-penetrating radar (GPR) surveys were used to accomplish these objectives. A description of the equipment used and a theoretical discussion of the geophysical methods are presented Appendix A. Because of the large number of figures relative to the number of pages of text, the geophysical grid-location maps, the contour maps of the magnetic-gradient data, the contour maps of the EM conductivity data, and the GPR traverse location maps are located in Appendix B, Tabs I through 22. In addition, selected GPR records are located in Appendix C

Guideline for environmental site investigations in Manitoba : June 1998

International Nuclear Information System (INIS)

1998-06-01

The procedures for the investigation and characterization of contaminated sites in Manitoba are described in an effort to provide a baseline for developing applicable risk-based remedial action and management plans. This document presents information regarding the methods and protocols for sites where the quality of groundwater, surface water, sediments or soil may have been affected by pollutants as a result of past or present usage of the site. The 1998 document focuses on petroleum impacted sites and also includes the requirements for the investigation of sites potentially impacted by other pollutants. It is based on the principles established by the Canadian Council of Ministers of the Environment for the management of sites in Canada and the requirements of the Manitoba Contaminated Sites Remediation and Consequential Amendments Act. Manitoba's petroleum storage program and the hazardous waste management program, including the disposal or treatment of contaminated soils are also summarized

Preliminary site description Laxemar stage 2.1. Feedback for completion of the site investigation including input from safety assessment and repository engineering

International Nuclear Information System (INIS)

2006-09-01

assessment possible on the basis of Laxemar 1.2 data. The list of site-specific critical issues is largely considered being covered by the complete site investigation programme. The added discipline-wise activities are expected to answer up to these issues/uncertainties. Many of the findings, some being of tentative nature, will be followed up more closely in the ensuing modelling steps

Managing Siting Activities for Nuclear Power Plants

International Nuclear Information System (INIS)

2012-01-01

, referenced bank of data that can be used during the lifetime of the nuclear power plant. As a result, and after reviewing the existing publications, the IAEA decided to develop an integrated Nuclear Energy Series publication on managing siting activities for a nuclear power plant using recent siting experience from States. This publication: -Complements the IAEA Safety Guide related to site selection; - Emphasizes the integrity and interdependence of various activities related to site selection and assessment, including safety, environmental, technical, economic and social factors; - Develops the methodology and framework for all aspects of the siting process; - Updates the existing IAEA documentation in order to better reflect the developments in the nuclear and energy industry related to siting; - Integrates the existing IAEA publications on the subject into a more compact and user friendly guide. This publication provides the information necessary to organize, guide and realize the activities related to the selection and assessment of a site through defining the factors for consideration and methodologies for site investigation. Other factors that could have an impact on the final selection, such as national or international politics are not addressed. This publication also discusses communication with and involvement of stakeholders as this has a strong influence on the process of selecting a site for a nuclear power plant. This report is intended for use by Member States initiating, restarting or expanding their nuclear power programmes. It gives guidance on the complex organizational, engineering, socio-economic, and environmental issues of siting. It aims to enable States to develop detailed country-specific processes for implementation of siting activities or to improve the efficiency and effectiveness of the siting process. This publication may be used by utilities assessing the possibilities for building and operating a nuclear power plant as well as by contractors

Managing Siting Activities for Nuclear Power Plants

Energy Technology Data Exchange (ETDEWEB)

NONE

2012-06-15

, referenced bank of data that can be used during the lifetime of the nuclear power plant. As a result, and after reviewing the existing publications, the IAEA decided to develop an integrated Nuclear Energy Series publication on managing siting activities for a nuclear power plant using recent siting experience from States. This publication: -Complements the IAEA Safety Guide related to site selection; - Emphasizes the integrity and interdependence of various activities related to site selection and assessment, including safety, environmental, technical, economic and social factors; - Develops the methodology and framework for all aspects of the siting process; - Updates the existing IAEA documentation in order to better reflect the developments in the nuclear and energy industry related to siting; - Integrates the existing IAEA publications on the subject into a more compact and user friendly guide. This publication provides the information necessary to organize, guide and realize the activities related to the selection and assessment of a site through defining the factors for consideration and methodologies for site investigation. Other factors that could have an impact on the final selection, such as national or international politics are not addressed. This publication also discusses communication with and involvement of stakeholders as this has a strong influence on the process of selecting a site for a nuclear power plant. This report is intended for use by Member States initiating, restarting or expanding their nuclear power programmes. It gives guidance on the complex organizational, engineering, socio-economic, and environmental issues of siting. It aims to enable States to develop detailed country-specific processes for implementation of siting activities or to improve the efficiency and effectiveness of the siting process. This publication may be used by utilities assessing the possibilities for building and operating a nuclear power plant as well as by contractors

Contaminated site investigation using nuclear technique: a case study of temporary transformer storage sites in Ghana

International Nuclear Information System (INIS)

Sanu, J. K.

2013-07-01

2000DX PCB/ Chloride analyser and Instrumentatal Neutron Activation Analysis (INAA) to analyse extractable organochloriine from the soil samples indicated that the nuclear technique is a better analytical technique for contaminated site investigation due to its high sensitivity, selectivity, fast and non-destructive nature. The INAA and gamma spectroscopy using HPGe detector coupled with MAESTRO 32 software provided a fast and efficient way to analyse possible PCB contamination in the soil samples and therefore proved to be very reliable method that could be conveniently used for contaminated site investigation. (au)

Final Report: Results of Environmental Site Investigation at Sylvan Grove, Kansas

Energy Technology Data Exchange (ETDEWEB)

LaFreniere, Lorraine M [Argonne National Lab. (ANL), Argonne, IL (United States)

2014-09-01

Sylvan Grove is located in western Lincoln County, approximately 60 mi west of Salina, Kansas (Figure 1.1). From 1954 to 1966, the Commodity Credit Corporation (CCC), an agency of the U.S. Department of Agriculture (USDA), operated a grain storage facility at the northeastern edge of Sylvan Grove. During this time, commercial grain fumigants containing carbon tetrachloride were in common use to preserve grain in storage. In 1998, the Kansas Department of Health and Environment (KDHE) found carbon tetrachloride above the maximum contaminant level (MCL) of 5 μg/L in groundwater from one private well used for livestock and lawn and garden watering. The 1998 KDHE sampling at Sylvan Grove was conducted under the USDA private well sampling program. To determine whether the former CCC/USDA facility at Sylvan Grove is a potential contaminant source and its possible relationship to the contamination in groundwater, the CCC/USDA proposed to conduct an environmental site investigation, in accordance with the Intergovernmental Agreement between the KDHE and the Farm Service Agency (FSA) of the USDA. Argonne National Laboratory, on behalf of the CCC/USDA, developed a work plan (Argonne 2012) for the site investigation and a supplemental work plan for indoor and ambient air sampling (Appendix A). The proposed work was approved by the KDHE (2012a, 2013). The investigations were performed by the Environmental Science Division of Argonne National Laboratory, on behalf of the CCC/USDA. The main activities for the site investigation were conducted in June 2012, and indoor and ambient air sampling was performed in February 2013. This report presents the findings of the investigations at Sylvan Grove.

A geotechnical investigation of a deep ocean site

International Nuclear Information System (INIS)

Freeman, T.J.; Schuttenhelm, R.T.E.

1990-01-01

A site investigation in deep water often relies solely on laboratory tests to evaluate the geotechnical properties of the sediments. This imposes two fundamental limitations on the investigation: The maximum depth to which the properties can be profiled and the uncertainty of sample disturbance and de-pressurization effects on the measured data. This paper uses results from investigations performed in a water depth of 5.4 km at an abyssal plain site, Great Meteor East (GME), to illustrate how ambiguities can arise in laboratory measurements of strength, and discusses how a simple in-situ test, the expendable penetrator, can be used to corroborate the laboratory data

Investigations at the former sites of raw phosphate processing in Germany

International Nuclear Information System (INIS)

Reichelt, Andreas; Weiss, Dietmar; Feige, Sebastian; Wiegand, Jens; Gerler, Juergen

2008-01-01

Raw phosphates have been processed in an industrial scale in Germany approximately since the middle of the 19th century. During the centuries, huge amounts of residues with high mass specific activities have been accumulated. Within the framework of a historical study, we have determined the accumulated radionuclide inventory from 1865 to 2005. The consumption amounts to approx. 150 million tons raw phosphates (corresponds to approx. 2.22*10 14 Bq U-238) with a fraction of national production of approx. 1 million tons. A production of approx. 3 million tons of elemental phosphorus (calcium silicate slag: 3.6*10 13 Bq Ra-226) and approx. 12 million tons of phosphoric acid (phosphogypsum: 2.7*10 13 Bq Ra-226) had been achieved. In the next step, we have looked for the former sites with legacies of the raw phosphate processing industry. 92 sites of former production of phosphate fertilizers, 3 sites of former production of elemental phosphorus and 10 sites of former production of phosphoric acid had been identified. Some of them were selected for more detailed researches regarding the accumulated radionuclide inventory and on-site/laboratory radiological investigations. (author)

A summary of INSITE activities in tracking SKB's spent fuel repository site investigations from 2002-2009 and of advice provided to the regulatory authorities on the status of site understanding at the end of the surface-based investigations

International Nuclear Information System (INIS)

Chapman, Neil; Bath, Adrian; Geier, Joel; Ove Stephansson; Tiren, Sven; Tsang, Chin-Fu

2010-11-01

SSM and its predecessor SKI employed a team of earth scientists who followed and reviewed SKB's investigations of the potential spent nuclear fuel repository sites at Forsmark and Laxemar. This group was named INSITE (INdependent Site Investigation Tracking and Evaluation) and began its work in 2002 and completed its task with the review of the final versions SKB's site descriptive models, SDM-Site, in 2009. This report is a summary of INSITE's work over the eight-and-a-half year period of the site investigations and the lead-in and the wind-down to the work. It is intended to provide an outline and a record of how INSITE has worked and how its advice was generated and provided to SKI and, latterly, to SSM. Together with all the other documentation generated by INSITE, this report is intended to support the regulatory review of SKB's licence application for a spent nuclear fuel repository

The role of historical operations information for supporting remedial investigation work at the former Harshaw Chemical Site

International Nuclear Information System (INIS)

Johnson, R.; Peterson, J.; Picel, K.; Kolhoff, A.; Devaughn, J.

2008-01-01

In the early stages of hazardous, toxic, and radioactive waste (HTRW) site investigations, basic record searches are performed to help direct the agencies investigating contaminated sites to areas of concern and to identify contaminants of interest (COI). Plans developed on the basis of this preliminary research alone are often incomplete and result in unexpected discoveries either while in the field investigating the site or after the reports have been written. Many of the sites investigated under the Formerly Utilized Sites Remedial Action program (FUSRAP) have complex histories that are slowly uncovered over the life of the project. Because of programmatic constraints, nuances of these sites are often discovered late in their programs and result in increased expenditures in order to fully characterize the site, perform a robust feasibility study, and recommend appropriate alternatives for remediation. By identifying resources for public records, classified records, historic aerial photographs, and other sources of site-specific historical information, a process can be established to optimize the collection of information and to develop efficient and complete project plans. In many cases, interviews with past site employees are very useful tools. In combining what is found in the records, observed on historic aerial photographs, and heard from former employees and family members, teams investigating these sites can begin to compile sound and more complete conceptual site models (CSMs). The former Harshaw Chemical Site (HCS) illustrates this discovery process. HCS is part of FUSRAP. Preliminary investigations by the US Department of Energy (DOE) in the 1970s provided an initial CSM of activities that had taken place that may have resulted in contamination. The remedial investigation (RI) conducted by the US Army Corps of Engineers (USACE) was designed around this CSM. The RI work, however, identified a number of site conditions that were unexpected, including new

“Doing the Wrong Things Right” Site Investigations in Soft Soil

Science.gov (United States)

Jamilus, M. H.; Lim, A. J. M. S.; Azhar, A. T. S.; Azmi, M. A. M.

2016-11-01

Site investigation is a very important process by which geotechnical, geological and other relevant information which might affect the construction or performance of a civil engineering or building project is acquired. However, common practice in site investigations is not always in accordance to the standard that has been defined. Reliability on the information obtained depends upon several factors that involves correct procedures and competent workers and also supervision. Several examples on site investigation methods are discussed in this paper. Explanation on the difference between the site investigation methods used for real practices in the field and how it should be done are discussed in detail. Therefore, it is hoped that site investigation should always be uniquely planned and should be an interactive and flexible process of discovery and changes according to the condition of the soil.

Thermal Site Descriptive Model. A strategy for the model development during site investigations. Version 1.0

International Nuclear Information System (INIS)

Sundberg, Jan

2003-04-01

Site investigations are in progress for the siting of a deep repository for spent nuclear fuel. As part of the planning work, strategies are developed for site descriptive modelling regarding different disciplines, amongst them the thermal conditions. The objective of the strategy for a thermal site descriptive model is to guide the practical implementation of evaluating site specific data during the site investigations. It is understood that further development may be needed. The model describes the thermal properties and other thermal parameters of intact rock, fractures and fracture zones, and of the rock mass. The methodology is based on estimation of thermal properties of intact rock and discontinuities, using both empirical and theoretical/numerical approaches, and estimation of thermal processes using mathematical modelling. The methodology will be used and evaluated for the thermal site descriptive modelling at the Aespoe Hard Rock Laboratory

Preliminary site description Laxemar stage 2.1. Feedback for completion of the site investigation including input from safety assessment and repository engineering

Energy Technology Data Exchange (ETDEWEB)

NONE

2006-09-15

with the assessment possible on the basis of Laxemar 1.2 data. The list of site-specific critical issues is largely considered being covered by the complete site investigation programme. The added discipline-wise activities are expected to answer up to these issues/uncertainties. Many of the findings, some being of tentative nature, will be followed up more closely in the ensuing modelling steps.

Geological Site Descriptive Model. A strategy for the model development during site investigations

Energy Technology Data Exchange (ETDEWEB)

Munier, Raymond; Stenberg, Leif [Swedish Nuclear Fuel and Waste Management Co., Stockholm (Sweden); Stanfors, Roy [Roy Stanfors Consulting, Lund (Sweden); Milnes, Allan Geoffrey [GEA Consulting, Uppsala (Sweden); Hermanson, Jan [Golder Associates, Stockholm (Sweden); Triumf, Carl-Axel [Geovista, Luleaa (Sweden)

2003-04-01

The Swedish Nuclear Fuel and Waste Management Company (SKB) is at present conducting site investigations as a preliminary to building an underground nuclear waste disposal facility in Sweden. This report presents a methodology for constructing, visualising and presenting 3-dimensional geological models, based on data from the site investigations. The methodology integrates with the overall work-flow of the site investigations, from the collection of raw data to the complete site description, as proposed in several earlier technical reports. Further, it is specifically designed for interaction with SICADA - SKB's Site Characterisation Database - and RVS - SKB's Rock Visualisation System. This report is one in a series of strategy documents intended to demonstrate how modelling is to be performed within each discipline. However, it also has a wider purpose, since the geological site descriptive model provides the basic geometrical framework for all the other disciplines. Hence, the wider aim is to present a practical and clear methodology for the analysis and interpretation of input data for use in the construction of the geology-based 3D geometrical model. In addition to the various aspects of modelling described above, the methodology presented here should therefore also provide: guidelines and directives on how systematic interpretation and integration of geo-scientific data from the different investigation methods should be carried out; guidelines on how different geometries should be created in the geological models; guidelines on how the assignment of parameters to the different geological units in RVS should be accomplished; guidelines on the handling of uncertainty at different points in the interpretation process. In addition, it should clarify the relation between the geological model and other models used in the processes of site characterisation, repository layout and safety analysis. In particular, integration and transparency should be

Geological Site Descriptive Model. A strategy for the model development during site investigations

International Nuclear Information System (INIS)

Munier, Raymond; Stenberg, Leif; Stanfors, Roy; Milnes, Allan Geoffrey; Hermanson, Jan; Triumf, Carl-Axel

2003-04-01

The Swedish Nuclear Fuel and Waste Management Company (SKB) is at present conducting site investigations as a preliminary to building an underground nuclear waste disposal facility in Sweden. This report presents a methodology for constructing, visualising and presenting 3-dimensional geological models, based on data from the site investigations. The methodology integrates with the overall work-flow of the site investigations, from the collection of raw data to the complete site description, as proposed in several earlier technical reports. Further, it is specifically designed for interaction with SICADA - SKB's Site Characterisation Database - and RVS - SKB's Rock Visualisation System. This report is one in a series of strategy documents intended to demonstrate how modelling is to be performed within each discipline. However, it also has a wider purpose, since the geological site descriptive model provides the basic geometrical framework for all the other disciplines. Hence, the wider aim is to present a practical and clear methodology for the analysis and interpretation of input data for use in the construction of the geology-based 3D geometrical model. In addition to the various aspects of modelling described above, the methodology presented here should therefore also provide: guidelines and directives on how systematic interpretation and integration of geo-scientific data from the different investigation methods should be carried out; guidelines on how different geometries should be created in the geological models; guidelines on how the assignment of parameters to the different geological units in RVS should be accomplished; guidelines on the handling of uncertainty at different points in the interpretation process. In addition, it should clarify the relation between the geological model and other models used in the processes of site characterisation, repository layout and safety analysis. In particular, integration and transparency should be promoted. The

SFR site investigation. Bedrock Hydrogeochemistry

International Nuclear Information System (INIS)

Nilsson, Ann-Chatrin; Tullborg, Eva-Lena; Smellie, John; Gimeno, Maria J.; Gomez, Javier B.; Auque, Luis F.; Sandstroem, Bjoern; Pedersen, Karsten

2011-11-01

There are plans that the final repository for low and intermediate level radioactive waste, SFR, located about 150 km north of Stockholm, will be extended. Geoscientific studies to define and characterise a suitable bedrock volume for the extended repository have been carried out from 2007 to 2011, and have included the drilling and evaluation of seven new core drilled and four percussion boreholes. These new data, together with existing data extending back to 1985, have been interpreted and modelled in order to provide the necessary information for safety assessment and repository design. This report presents the final hydrogeochemical site description for the SFR site, and will constitute a background report for the integrated site description (the SFR Site Descriptive Model version 1.0) together with corresponding reports from the geological and hydrogeological disciplines. Most of the hydrogeochemical data from the field investigations consist of major ions and isotopes together with sporadic gas, microbe and measured redox data. Despite the close proximity of the Forsmark site, few data from this source are of relevance because of the shallow nature of the SFR site, the fact that SFR is located beneath the Baltic Sea and also the drawdown/upconing impacts of its construction on the hydrogeochemistry. This artificially imposed dynamic flow system is naturally more prevalent along major deformation fracture zones of higher transmissivity, whilst lower transmissive fractures together with the less transmissive bedrock masses between major deformation zones, still retain some evidence of the natural groundwater mixing patterns established prior to the SFR construction. The groundwaters in the SFR dataset cover a depth down to -250 m.a.s.l. with single sampling locations at -300 and -400 m.a.s.l. and represent a relatively limited salinity range (1,500 to 5,500 mg/L chloride). However, the δ 18 O values show a wide variation (-15.5 to -7.5 per mille V

SFR site investigation. Bedrock Hydrogeochemistry

Energy Technology Data Exchange (ETDEWEB)

Nilsson, Ann-Chatrin [Geosigma AB, Uppsala (Sweden); Tullborg, Eva-Lena [Terralogica AB, Graabo (Sweden); Smellie, John [Conterra AB, Uppsala (Sweden); Gimeno, Maria J.; Gomez, Javier B.; Auque, Luis F. [Univ. of Zaragoza, Zaragoza (Spain); Sandstroem, Bjoern [WSP Sverige AB, Goeteborg (Sweden); Pedersen, Karsten [Micans AB, Moelnlycke (Sweden)

2011-11-15

There are plans that the final repository for low and intermediate level radioactive waste, SFR, located about 150 km north of Stockholm, will be extended. Geoscientific studies to define and characterise a suitable bedrock volume for the extended repository have been carried out from 2007 to 2011, and have included the drilling and evaluation of seven new core drilled and four percussion boreholes. These new data, together with existing data extending back to 1985, have been interpreted and modelled in order to provide the necessary information for safety assessment and repository design. This report presents the final hydrogeochemical site description for the SFR site, and will constitute a background report for the integrated site description (the SFR Site Descriptive Model version 1.0) together with corresponding reports from the geological and hydrogeological disciplines. Most of the hydrogeochemical data from the field investigations consist of major ions and isotopes together with sporadic gas, microbe and measured redox data. Despite the close proximity of the Forsmark site, few data from this source are of relevance because of the shallow nature of the SFR site, the fact that SFR is located beneath the Baltic Sea and also the drawdown/upconing impacts of its construction on the hydrogeochemistry. This artificially imposed dynamic flow system is naturally more prevalent along major deformation fracture zones of higher transmissivity, whilst lower transmissive fractures together with the less transmissive bedrock masses between major deformation zones, still retain some evidence of the natural groundwater mixing patterns established prior to the SFR construction. The groundwaters in the SFR dataset cover a depth down to -250 m.a.s.l. with single sampling locations at -300 and -400 m.a.s.l. and represent a relatively limited salinity range (1,500 to 5,500 mg/L chloride). However, the {delta}{sup 18}O values show a wide variation (-15.5 to -7.5 per mille V

Efficient oxygen electrocatalysis on special active sites

DEFF Research Database (Denmark)

Halck, Niels Bendtsen

throughout this thesis to understand these local structure effects and their influence on surface reactions. The concept of these special active sites is used to explain how oxygen evolution reaction (OER) catalysts can have activities beyond the limits of what was previously thought possible. The concept...... stored in these bonds in an eco-friendly fashion in fuel cells. This thesis explores catalysts for oxygen electrocatalysis and how carefully designed local structures on catalysts surfaces termed special active sites can influence the activity. Density functional theory has been used as a method...... is used to explain the increase in activity observed for the OER catalyst ruthenium dioxide when it is mixed with nickel or cobalt. Manganese and cobalt oxides when in the vicinity of gold also display an increase in OER activity which can be explained by locally created special active sites. Density...

Assessing Contractor Capabilities for Streamlined Site Investigations

Science.gov (United States)

The purpose of this document is to familiarize and encourage brownfields decision makers to investigate and employ innovative methods for characterizing their sites, to assist brownfields decision makers in assessing contractors' capabilities.

The Lusi eruption site: insights from surface and subsurface investigations

Science.gov (United States)

Mazzini, A.

2017-12-01

The Indonesian Lusi eruption has been spewing boiling water, gas, and sediments since the 29th of May 2006. Initially, numerous aligned eruptions sites appeared along the Watukosek fault system (WFS) that was reactivated after the Yogyakarta earthquake occurring the 27th of May in the Java Island. Within weeks several villages were submerged by boiling mud. The most prominent eruption site was named Lusi. To date Lusi is still active and an area of 7 km2is covered by mud. Since its birth Lusi erupted with a pulsating behaviour. In the framework of the ERC grant "Lusi Lab" we conducted several years of monitoring and regional investigations coupling surface sampling and subsurface imaging in the region around Lusi. Ambient noise tomography studies, obtained with a local network of 31 stations, revealed for the first time subsurface images of the Lusi region and the adjacent Arjuno-Welirang (AW) volcanic complex. Results show that below the AW volcanic complex are present 5km deep magma chambers that are connected, through a defined corridor, with the roots of the Lusi eruption site. The Lusi subsurface shows the presence of a defined vertical hydrothermal plume that extends to at least 5km. Chemical analyses of the seeping fluids sampled from 1) the Lusi plume (using a specifically designed drone), 2) the region around Lusi, and 3) the fumaroles and the hydro thermal springs of AW, revealed striking similarities. More specifically a mantellic signature of the Lusi fluids confirms the scenario that Lusi represents a magmatic-driven hydrothermal system hosted in sedimentary basin. Seismic profiles interpretation, surface mapping, and fluid sampling show that the WFS, connecting AW and extending towards the NE of Java, acted as a preferential pathway for the igneous intrusion and fluids migration towards the subsurface. Petrography and dating of the clasts erupted at Lusi record high temperatures and indicate that the roots of the active conduit extend to at least 5km

Recent Experience Using Active Love Wave Techniques to Characterize Seismographic Station Sites

Science.gov (United States)

Martin, A. J.; Yong, A.; Salomone, L.

2014-12-01

Active-source Love waves recorded by the multi-channel analysis of surface wave (MASLW) technique were recently analyzed in two site characterization projects. Between 2010 and 2011, the 2009 American Recovery and Reinvestment Act (ARRA) funded GEOVision to conduct geophysical investigations at 189 seismographic stations—185 in California and 4 in the Central Eastern U.S. (CEUS). The original project plan was to utilize active and passive Rayleigh wave-based techniques to obtain shear-wave velocity (VS) profiles to a minimum depth of 30 m and the time-averaged VS of the upper 30 meters (VS30). Early in the investigation it became evident that Rayleigh wave techniques, such as multi-channel analysis of surface waves (MASRW), were not effective at characterizing all sites. Shear-wave seismic refraction and MASLW techniques were therefore applied. The MASLW technique was deployed at a total of 38 sites, in addition to other methods, and used as the primary technique to characterize 22 sites, 5 of which were also characterized using Rayleigh wave techniques. In 2012, the Electric Power Research Institute funded characterization of 33 CEUS station sites. Based on experience from the ARRA investigation, both MASRW and MASLW data were acquired by GEOVision at 24 CEUS sites—the remaining 9 sites and 2 overlapping sites were characterized by University of Texas, Austin. Of the 24 sites characterized by GEOVision, 16 were characterized using MASLW data, 4 using both MASLW and MASRW data and 4 using MASRW data. Love wave techniques were often found to perform better, or at least yield phase velocity data that could be more readily modeled using the fundamental mode assumption, at shallow rock sites, sites with steep velocity gradients, and, sites with a thin, low velocity, surficial soil layer overlying stiffer sediments. These types of velocity structure often excite dominant higher modes in Rayleigh wave data, but not in Love wave data. At such sites, it may be possible

Shear wave velocity investigation of soil liquefaction sites from the Tangshan, China M7.8 earthquake of 1976 using active and passive surface wave methods

Science.gov (United States)

Kayen, Robert E; Tao, Xiaxin; Shi, Lijing; Shi, Hailiang

2008-01-01

An initial investigation of soil liquefaction sites from the July, 28 1976 Tangshan M7.8 earthquake was conducted between 1976 and 1978 by the National Ministry of Railways, China. These data are the basis of the ‘Chinese Method’ for assessment of liquefaction potential of silty-sand deposits, and are an important component of the worldwide data set for modern probabilistic methods for assessment of soil liquefaction using Bayesian updating and system reliability tools. We revisited 26 sites identified in the maps and published 198 report of the Ministry of Railways in order to investigate these locations with a suite of active- and passive-array surface wave methods. These sites are clustered along the north coast of the Bo Hai Sea in three areas: Lutai, Tianjin; Tangshan City and outlying village, Hebei; and Luannan county, Hebei. First, we gathered and evaluated the Rayleigh wave dispersion characteristics of the ground by comparing dispersion curves from the active source harmonic wave-spectral analysis of surface waves (SASW) method and the passive array Spatial Auto-Correlation method (SPAC). The dispersive properties of the liquefied ground as measured by these two methods were found to be almost identical. These tests were hybridized and the data sets merged in order to invert of shear wave velocities for analysis of liquefaction potential using a probabilistic framework. The data from high-values of seismic intensity near Tangshan city to low-intensities distant of the event in Luannan County segregate out into clusters of liquefied and non liquefied points clearly separated by liquefaction boundary curves developed from a large global data set of 310 sites

Developing a programmatic approach to investigating and remediating many unrelated comprehensive environmental response, compensation, and liability act sites at Kelly Air Force Base

International Nuclear Information System (INIS)

Kamp, G.; Regan, P.; Ninesteel, R.; Martin, R.

1988-01-01

Kelly Air Force Base (AFB), which was founded in 1917, is involved in logistics and maintenance activities supporting the Air Logistics Command. In addition, Kelly AFB hosts over 50 tenant organizations representing the Air Force, Department of Defense, and other government agencies. Over the years waste disposal from this complex was conducted in a manner that led to the identification of over 30 sites to be included in the Installation Restoration Program (IRP) after the Phase 1 investigation. A methodology was needed to prioritize the Remedial Investigations and Feasibility Study (RI/FS) activities for the sites. A Strategy Plan was developed that involved reviewing and interpreting existing data, identifying data voids relative to site specific RI/FS activities, and developing methodology to prioritize activities. Sites were prioritized, and a comprehensive IRP planning document was developed. One data deficiency was revealed -- the lack of understanding of the Basewide hydrogeologic conditions necessary to establish an effective restoration program. A Hydrogeologic Investigation was initiated to provide this data. This data will allow better interpretation of the interaction of the sites, particularly those in close proximity, and improved planning of remediation activities

Animal investigation program: Nevada test site and vicinity. Annual report, 1977

International Nuclear Information System (INIS)

Smith, D.D.; Crockett, A.B.; Bernhardt, D.E.; Giles, K.R.; Kinnison, R.R.

1979-07-01

Data are presented from the radioanalysis of tissues collected from cattle, mule deer, desert bighorn sheep, rabbits, feral horses, and other wildlife that resided on or near the Nevada Test Site. Routine activities and special investigations of the Animal Investigation Program are also discussed. Other than potassium-40, gamma-emitting radionuclides were detected infrequently. Strontium-90 concentrations in bones from deer, cattle, and desert bighorn sheep continued the downward trend of recent years. Tritium concentrations were generally within expected environmental limits with the exception of animals exposed to sources of contamination. Radionuclide tissue concentrations were generally higher in the tissues of animals residing in Area 15 than in other areas. Statistical analyses made of plutonium-239 levels reported in cattle tissue collected from 1971 through 1977 reveal that activity levels in lungs, liver, and bone are significantly related to age. Activity levels did not change significantly in the ingesta and lungs during this time but did tend to increase for bone and liver. Activity levels in the ingesta are significantly higher in the fall than in the spring. Hypothetical dose estimates to man were calculated on the basis of liver or muscle from animals that contained peak radionuclide levels. The highest postulated dose was 8.6 millirems for tritium in tissues from a mule deer. The movements of 17 mule deer were monitored on a weekly basis.During the winter months, all deer left their summer range on the mesas of the Nevada Test Site and migrated 40 to 60 kilometers south and west. A statistical estimate was made of the deer population in selected areas utilizing the marked deer as a basis for this estimate. No gross or microscopic lesions were found in necropsied animals that could be directly attributed to the effects of ionizing radiation

AECL strategy for surface-based investigations of potential disposal sites and the development of a geosphere model for a site

International Nuclear Information System (INIS)

Whitaker, S.H.; Brown, A.; Davison, C.C.; Gascoyne, M.; Lodha, G.S.; Stevenson, D.R.; Thorne, G.A.; Tomsons, D.

1994-05-01

The objective of this report is to summarize AECL's strategy for surface-based geotechnical site investigations used in screening and evaluating candidate areas and candidate sites for a nuclear fuel waste repository and for the development of geosphere models of sites. The report is one of several prepared by national nuclear fuel waste management programs for the Swedish Nuclear Fuel and Waste Management Co. (SKB) to provide international background on site investigations for SKB's R and D programme on siting.The scope of the report is limited to surface-based investigations of the geosphere, those done at surface or in boreholes drilled from surface. The report discusses AECL's investigation strategy and the methods proposed for use in surface-based reconnaissance and detailed site investigations at potential repository sites. Site investigations done for AECL's Underground Research Laboratory are used to illustrate the approach. The report also discusses AECL's strategy for developing conceptual and mathematical models of geological conditions at sites and the use of these models in developing a model (Geosphere Model) for use in assessing the performance of the disposal system after a repository is closed. Models based on the site data obtained at the URL are used to illustrate the approach. Finally, the report summarizes the lessons learned from AECL's R and D program on site investigations and mentions some recent developments in the R and D program. 120 refs, 33 figs, 7 tabs

AECL strategy for surface-based investigations of potential disposal sites and the development of a geosphere model for a site

Energy Technology Data Exchange (ETDEWEB)

Whitaker, S H; Brown, A; Davison, C C; Gascoyne, M; Lodha, G S; Stevenson, D R; Thorne, G A; Tomsons, D [AECL Research, Whiteshell Labs., Pinawa, MB (Canada)

1994-05-01

The objective of this report is to summarize AECL`s strategy for surface-based geotechnical site investigations used in screening and evaluating candidate areas and candidate sites for a nuclear fuel waste repository and for the development of geosphere models of sites. The report is one of several prepared by national nuclear fuel waste management programs for the Swedish Nuclear Fuel and Waste Management Co. (SKB) to provide international background on site investigations for SKB`s R and D programme on siting.The scope of the report is limited to surface-based investigations of the geosphere, those done at surface or in boreholes drilled from surface. The report discusses AECL`s investigation strategy and the methods proposed for use in surface-based reconnaissance and detailed site investigations at potential repository sites. Site investigations done for AECL`s Underground Research Laboratory are used to illustrate the approach. The report also discusses AECL`s strategy for developing conceptual and mathematical models of geological conditions at sites and the use of these models in developing a model (Geosphere Model) for use in assessing the performance of the disposal system after a repository is closed. Models based on the site data obtained at the URL are used to illustrate the approach. Finally, the report summarizes the lessons learned from AECL`s R and D program on site investigations and mentions some recent developments in the R and D program. 120 refs, 33 figs, 7 tabs.

Preliminary site characterization radiological monitoring plan for the Nevada Nuclear Waste Storage Investigations Project, Yucca Mountain Site

International Nuclear Information System (INIS)

1987-03-01

The activities described in this plan occur in the early phases of site characterization. This document presents the Preliminary Site Characterization Radiological Monitoring Plan (PSCRMP) for collecting and evaluating data in support of the NNWSI Project. The PSCRMP defines and identifies control procedures for the monitoring activities. The PSCRMP activity will utilize integrating radon monitoring devices, a continuous radon monitor, and a particulate air sampler. These instruments will be used to establish the baseline radioactivity and/or radioactivity released due to early site characterization activities. The sections that follow provide a general project description, the specifics of the monitoring program, and the practices that will be employed to ensure the validity of the collected data by integrating quality assurance into all activities. Section 2 of this document describes the regulatory base of this document. Section 3 describes the site characterization activities which may lead to release of radioactivity. Section 4 provides a description of the potential sources of radioactivity that site characterization could generate. Section 5 summarizes the sampling and monitoring methodology, which will be used to monitor the potential sources of radioactivity. The network of sampling and monitoring equipment is described in Section 6, and Section 7 summarizes the systems operation activities. The data reporting activities are described in Section 8. Finally, a description of the Quality Assurance (QA) and Quality Control (QC) activities is provided in Section 9. Appendix A contains a summary of the procedures to be used in this program, and Appendix B contains technical specification on equipment and services. 20 refs., 11 figs., 2 tabs

Safety Oversight of Decommissioning Activities at DOE Nuclear Sites

International Nuclear Information System (INIS)

Zull, Lawrence M.; Yeniscavich, William

2008-01-01

The Defense Nuclear Facilities Safety Board (Board) is an independent federal agency established by Congress in 1988 to provide nuclear safety oversight of activities at U.S. Department of Energy (DOE) defense nuclear facilities. The activities under the Board's jurisdiction include the design, construction, startup, operation, and decommissioning of defense nuclear facilities at DOE sites. This paper reviews the Board's safety oversight of decommissioning activities at DOE sites, identifies the safety problems observed, and discusses Board initiatives to improve the safety of decommissioning activities at DOE sites. The decommissioning of former defense nuclear facilities has reduced the risk of radioactive material contamination and exposure to the public and site workers. In general, efforts to perform decommissioning work at DOE defense nuclear sites have been successful, and contractors performing decommissioning work have a good safety record. Decommissioning activities have recently been completed at sites identified for closure, including the Rocky Flats Environmental Technology Site, the Fernald Closure Project, and the Miamisburg Closure Project (the Mound site). The Rocky Flats and Fernald sites, which produced plutonium parts and uranium materials for defense needs (respectively), have been turned into wildlife refuges. The Mound site, which performed R and D activities on nuclear materials, has been converted into an industrial and technology park called the Mound Advanced Technology Center. The DOE Office of Legacy Management is responsible for the long term stewardship of these former EM sites. The Board has reviewed many decommissioning activities, and noted that there are valuable lessons learned that can benefit both DOE and the contractor. As part of its ongoing safety oversight responsibilities, the Board and its staff will continue to review the safety of DOE and contractor decommissioning activities at DOE defense nuclear sites

Corrective Action Investigation Plan for Corrective Action Unit 190: Contaminated Waste Sites Nevada Test Site, Nevada, Rev. No.: 0

International Nuclear Information System (INIS)

Wickline, Alfred

2006-01-01

Corrective Action Unit (CAU) 190 is located in Areas 11 and 14 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 190 is comprised of the four Corrective Action Sites (CASs) listed below: (1) 11-02-01, Underground Centrifuge; (2) 11-02-02, Drain Lines and Outfall; (3) 11-59-01, Tweezer Facility Septic System; and (4) 14-23-01, LTU-6 Test Area. These sites are being investigated because existing information is insufficient on the nature and extent of potential contamination to evaluate and recommend corrective action alternatives. Additional information will be obtained before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS by conducting a corrective action investigation (CAI). The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on August 24, 2006, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture, and National Security Technologies, LLC. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 190. The scope of the CAU 190 CAI includes the following activities: (1) Move surface debris and/or materials, as needed, to facilitate sampling; (2) Conduct radiological and geophysical surveys; (3) Perform field screening; (4) Collect and submit environmental samples for laboratory analysis to determine whether contaminants of concern (COCs) are present; (5) If COCs are present, collect additional step-out samples to define the lateral and vertical extent of the contamination; (6) Collect samples of source material, if present

A summary of INSITE activities in tracking SKB's spent fuel repository site investigations from 2002-2009 and of advice provided to the regulatory authorities on the status of site understanding at the end of the surface-based investigations

Energy Technology Data Exchange (ETDEWEB)

Chapman, Neil (Chapman Consulting (Switzerland)); Bath, Adrian (Intellisci Ltd, (United Kingdom)); Geier, Joel (Clearwater Hardrock Consulting (United States)); Ove Stephansson (Steph Rock Consulting AB (Sweden)); Tiren, Sven (Geosigma (Sweden)); Tsang, Chin-Fu (Berkeley Geohydrophysics SP (United States))

2010-11-15

SSM and its predecessor SKI employed a team of earth scientists who followed and reviewed SKB's investigations of the potential spent nuclear fuel repository sites at Forsmark and Laxemar. This group was named INSITE (INdependent Site Investigation Tracking and Evaluation) and began its work in 2002 and completed its task with the review of the final versions SKB's site descriptive models, SDM-Site, in 2009. This report is a summary of INSITE's work over the eight-and-a-half year period of the site investigations and the lead-in and the wind-down to the work. It is intended to provide an outline and a record of how INSITE has worked and how its advice was generated and provided to SKI and, latterly, to SSM. Together with all the other documentation generated by INSITE, this report is intended to support the regulatory review of SKB's licence application for a spent nuclear fuel repository

Good clinical practice regulatory inspections: Lessons for Indian investigator sites

Directory of Open Access Journals (Sweden)

R Marwah

2010-01-01

Full Text Available Regulatory inspections are important to evaluate the integrity of the data submitted to health authorities (HAs, protect patient safety, and assess adequacy of site/sponsor quality systems to achieve the same. Inspections generally occur after submission of data for marketing approval of an investigational drug. In recent years, there has been a significant increase in number of inspections by different HAs, including in India. The assessors/inspectors generally do a thorough review of site data before inspections. All aspects of ICH-GCP, site infrastructure, and quality control systems are assessed during the inspection. Findings are discussed during the close out meeting and a detailed inspection report issued afterward, which has to be responded to within 15-30 days with effective Corrective and Preventive Action Plan (CAPA. Protocol noncompliance, inadequate/inaccurate records, inadequate drug accountability, informed consent issues, and adverse event reporting were some of the most common findings observed during recent Food and Drug Administration (FDA inspections. Drug development is being increasingly globalized and an increased number of patients enrolled in studies submitted as part of applications come from all over the world including India. Because of the steep increase in research activity in the country, inexperienced sites, and more stakeholders, increased efforts will be required to ensure continuous quality and compliance. HAs have also made clear that enforcement will be increased and be swift, aggressive, and effective.

Development of the web-based site investigation flow diagram in repository development program

International Nuclear Information System (INIS)

Yamamoto, Shuichi; Yoshimura, Kimitaka; Ohuchi, Jin; Tsuboya, Takao; Ando, Kenichi

2005-01-01

In siting a repository for high level radioactive wastes (HLW), it is essential for consensus building intelligibly and visually present why and how the area is selected as a suitable site. However 'information asymmetry' exists especially between society and an implementation body because various types of investigation, analysis and assessment are implemented in site characterization on the basis of a wide variety of advanced science and technology. Communication between experts (e.g. surveyors and modelers) is also important for efficient and reliable site investigation/ characterization. The Web-based Site Investigation Flow Diagram (SIFD) has been developed as a tool for information sharing among stake holders and society-jointed decision making. To test applicability of the SIFD, virtual site characterization ('dry run') is performed using the existing site investigation data. It is concluded that the web-based SIFD enhance traceability and transparency of the site investigation/ characterization, and therefore it would be a powerful communication tool among experts for efficient and reliable site investigation/characterization and among stake holders for consensus building

Corrective Action Investigation Plan for Corrective Action Unit 232: Area 25 Sewage Lagoons Nevada Test Site, Nevada

Energy Technology Data Exchange (ETDEWEB)

DOE/NV Operations Office

1999-05-01

This Corrective Action Investigation Plan (CAIP) has been developed in accordance with the Federal Facility Agreement and Consent Order (FFACO) (1996) that was agreed to by the US Department of Energy, Nevada Operations Office (DOE/NV); the State of Nevada Division of Environmental Protection (NDEP); and the US Department of Defense. The CAIP is a document that provides or references all of the specific information for investigation activities associated with Corrective Action Units (CAUs) or Corrective Action Sites (CASs). According to the FFACO, CASs are sites potentially requiring corrective action(s) and may include solid waste management units or individual disposal or release sites. A CAU consists of one or more CASs grouped together based on geography, technical similarity, or agency responsibility for the purpose of determining corrective actions. This CAIP contains the environmental sample collection objectives and criteria for conducting site investigation activities at CAU 232, Area 25 Sewage Lagoons. Corrective Action Unit 232 consists of CAS 25-03-01, Sewage Lagoon, located in Area 25 of the Nevada Test Site (NTS). The NTS is approximately 65 miles (mi) northwest of Las Vegas, Nevada (Figure 1-1) (DOE/NV, 1996a). The Area 25 Sewage Lagoons (Figure 1-2) (IT, 1999b) are located approximately 0.3 mi south of the Test Cell 'C' (TCC) Facility and were used for the discharge of sanitary effluent from the TCC facility. For purposes of this discussion, this site will be referred to as either CAU 232 or the sewage lagoons.

Status of remedial investigation activities in the Hanford Site 300 Area groundwater operable unit

International Nuclear Information System (INIS)

Hulstrom, L.C.; Innis, B.E.; Frank, M.A.

1993-09-01

The Phase 1 remedial investigation (RI) and Phase 1 and 2 feasibility studies (FS) for the 300-FF-5 groundwater operable unit underlying the 300 Area on the Hanford Site have been completed. Analysis and evaluation of soil, sediment, and surface water, and biotic sampling data, groundwater chemistry, and radiological data gathered over the past 3 years has been completed. Risk assessment calculations have been performed. Use of the data gathered, coupled with information from an automated water level data collection system, has enabled engineers to track three plumes that represent the most significant contamination of the groundwater

Final Phase II report : QuickSite(R) investigation, Everest, Kansas.

Energy Technology Data Exchange (ETDEWEB)

LaFreniere, L. M. (Environmental Research)

2003-11-01

field work at Everest. The main session of field work for the Phase II QuickSite{reg_sign} investigation of the Everest site began on March 6, 2001. Work was suspended at the site on April 6, 2001, (1) because of access limitations to key properties, located north and west of the former CCC/USDA facility, imposed by the private owners at the onset of the spring planting season and (2) to permit further documentation by Argonne, at the request of the CCC/USDA, of the land use and ownership history of the Nigh property as a precursor to completion of the field work. This period is termed the second session of Phase II field work at Everest. Investigation of the Nigh property history was prompted by groundwater contamination evidence obtained during the second session of Phase II field activities (discussed in Section 3.7).

Site investigation - equipment for geological, geophysical, hydrogeological and hydrochemical characterization

International Nuclear Information System (INIS)

Almen, K.E.; Fridh, B.; Johansson, B.E.; Sehlstedt, M.

1986-11-01

The investigations are performed within a site investigation program. In total about 60,000 m of cored 56 mm boreholes have been drilled and investigated at eight study sites. A summarized description of the main investigation methods is included. Instruments for geophysical investigations contains equipment for ground measurements as well as for borehole logging. The Geophysical investigations including the borehole radar measurements, are indirect methods for the geological and hydrogeological characterization of the rock formation. Great effort has been laid on the development of hydrogeological instruments for hydraulic tests and groundwater head measurements. In order to obtain hydrochemical investigations with high quality, a complete system for sampling and analysis of ground water has been developed. (orig./PW)

Dashboard applications to monitor experiment activities at sites

Energy Technology Data Exchange (ETDEWEB)

Andreeva, Julia; Gaidioz, Benjamin; Grigoras, Costin; Kokoszkiewicz, Lukasz; Lanciotti, Elisa; Rocha, Ricardo; Saiz, Pablo; Santinelli, Roberto; Sidorova, Irina; Sciaba, Andrea [CERN, European Organization for Nuclear Research (Switzerland); Belforte, Stefano [INFN Trieste (Italy); Boehm, Max [EDS, an HP Company, Plano, TX (United States); Casajus, Adrian [Universitat de Barcelona (Spain); Flix, Josep [PIC, Port d' Informacio CientIfica, Bellaterra (Spain); Tsaregorodtsev, Andrei, E-mail: Elisa.Lanciotti@cern.c, E-mail: Pablo.Saiz@cern.c [CPPM Marseille (France)

2010-04-01

In the framework of a distributed computing environment, such as WLCG, monitoring has a key role in order to keep under control activities going on in sites located in different countries and involving people based in many different sites. To be able to cope with such a large scale heterogeneous infrastructure, it is necessary to have monitoring tools providing a complete and reliable view of the overall performance of the sites. Moreover, the structure of a monitoring system critically depends on the object to monitor and on the users it is addressed to. In this article we will describe two different monitoring systems both aimed to monitor activities and services provided in the WLCG framework, but designed in order to meet the requirements of different users: Site Status Board has an overall view of the services available in all the sites supporting an experiment, whereas Siteview provides a complete view of all the activities going on at a site, for all the experiments supported by the site.

Dashboard applications to monitor experiment activities at sites

International Nuclear Information System (INIS)

Andreeva, Julia; Gaidioz, Benjamin; Grigoras, Costin; Kokoszkiewicz, Lukasz; Lanciotti, Elisa; Rocha, Ricardo; Saiz, Pablo; Santinelli, Roberto; Sidorova, Irina; Sciaba, Andrea; Belforte, Stefano; Boehm, Max; Casajus, Adrian; Flix, Josep; Tsaregorodtsev, Andrei

2010-01-01

In the framework of a distributed computing environment, such as WLCG, monitoring has a key role in order to keep under control activities going on in sites located in different countries and involving people based in many different sites. To be able to cope with such a large scale heterogeneous infrastructure, it is necessary to have monitoring tools providing a complete and reliable view of the overall performance of the sites. Moreover, the structure of a monitoring system critically depends on the object to monitor and on the users it is addressed to. In this article we will describe two different monitoring systems both aimed to monitor activities and services provided in the WLCG framework, but designed in order to meet the requirements of different users: Site Status Board has an overall view of the services available in all the sites supporting an experiment, whereas Siteview provides a complete view of all the activities going on at a site, for all the experiments supported by the site.

Preliminary site investigation for LL and IL radwaste disposal for Qinshan NPP

International Nuclear Information System (INIS)

Huang Yawen; Chen Zhangru

1993-01-01

With the purpose of selecting a disposal site for the low- and intermediate-level radwastes arising from Qinshan NPP, site investigations were carried out in several districts of Zhejiang Province. Investigation objectives included the circumstances of geology, hydrogeology, environmental ecology, and social economy. On the basis of collected data, five possible sites were recommended for policy-making reference and further investigation

Corrective Action Investigation Plan for Corrective Action Unit 139: Waste Disposal Sites, Nevada Test Site, Nevada

International Nuclear Information System (INIS)

Grant Evenson

2006-01-01

Corrective Action Unit (CAU) 139 is located in Areas 3, 4, 6, and 9 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 139 is comprised of the seven corrective action sites (CASs) listed below: (1) 03-35-01, Burn Pit; (2) 04-08-02, Waste Disposal Site; (3) 04-99-01, Contaminated Surface Debris; (4) 06-19-02, Waste Disposal Site/Burn Pit; (5) 06-19-03, Waste Disposal Trenches; (6) 09-23-01, Area 9 Gravel Gertie; and (7) 09-34-01, Underground Detection Station. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives with the exception of CASs 09-23-01 and 09-34-01. Regarding these two CASs, CAS 09-23-01 is a gravel gertie where a zero-yield test was conducted with all contamination confined to below ground within the area of the structure, and CAS 09-34-01 is an underground detection station where no contaminants are present. Additional information will be obtained by conducting a corrective action investigation (CAI) before evaluating corrective action alternatives and selecting the appropriate corrective action for the other five CASs where information is insufficient. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on January 4, 2006, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and Bechtel Nevada. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 139

Site description of the SFR area at Forsmark at completion of the site investigation phase. SDM-PSU Forsmark

Energy Technology Data Exchange (ETDEWEB)

NONE

2013-05-15

The site descriptive model (SDM) presented in this report is an integrated model for bedrock geology, rock mechanics, bedrock hydrogeology and bedrock hydrogeochemistry of the site investigated in the SFR extension project (PSU). A description of the surface system is also included in the report. However, the surface system is not integrated with the other disciplines as new data regarding the surface system will not be available until after the completion of SDM-PSU. It is noted that SDM-PSU does not include all disciplines handled in SDM-Site Forsmark (SKB 2008b), the focus is to produce a site description that meets the needs of the SFR extension project. The overall objective of the SFR extension project is to have the application for the extension ready by 2013. This report presents an integrated site model incorporating the historic data acquired from the investigations for and construction of the existing SFR facility (1980-1986), as well as from the recent investigations for the planned extension of SFR (2008-2009). It also provides a summary of the abundant underlying data and the discipline-specific models that support the integrated site model. The description relies heavily on background reports concerning detailed data analyses and modelling in the different disciplines. It is noteworthy that the investigations conducted during the SFR extension project were guided by the choice of site prior to the investigations, which was based on the experience gained during the construction of the existing SFR facility.

Site description of the SFR area at Forsmark at completion of the site investigation phase. SDM-PSU Forsmark

International Nuclear Information System (INIS)

2013-05-01

The site descriptive model (SDM) presented in this report is an integrated model for bedrock geology, rock mechanics, bedrock hydrogeology and bedrock hydrogeochemistry of the site investigated in the SFR extension project (PSU). A description of the surface system is also included in the report. However, the surface system is not integrated with the other disciplines as new data regarding the surface system will not be available until after the completion of SDM-PSU. It is noted that SDM-PSU does not include all disciplines handled in SDM-Site Forsmark (SKB 2008b), the focus is to produce a site description that meets the needs of the SFR extension project. The overall objective of the SFR extension project is to have the application for the extension ready by 2013. This report presents an integrated site model incorporating the historic data acquired from the investigations for and construction of the existing SFR facility (1980-1986), as well as from the recent investigations for the planned extension of SFR (2008-2009). It also provides a summary of the abundant underlying data and the discipline-specific models that support the integrated site model. The description relies heavily on background reports concerning detailed data analyses and modelling in the different disciplines. It is noteworthy that the investigations conducted during the SFR extension project were guided by the choice of site prior to the investigations, which was based on the experience gained during the construction of the existing SFR facility

Corrective Action Investigation Plan for Corrective Action Unit 366: Area 11 Plutonium Valley Dispersion Sites, Nevada National Security Site, Nevada

International Nuclear Information System (INIS)

Matthews, Patrick

2011-01-01

associated with the nuclear tests, it was determined that CASs 11-23-02, 11-23-03, and 11-23-04 will be investigated as one release site. The three test areas associated with these CASs are in close proximity; the devices tested were all composed of plutonium and enriched uranium; and the ground zeroes are all posted high contamination areas (HCAs). Because the device tested at CAS 11-23-01 was composed primarily of enriched uranium and the ground zero is not a posted HCA, the CAS will be investigated as a separate release. The DQO process also resulted in an assumption that TED within the HCAs and contaminated waste dumps exceeds the FAL and requires corrective action. A field investigation will be performed to define where TED exceeds the FAL and to determine whether other contaminants of concern are present at the site associated with other activities that took place at the site or from spills or waste discovered during the investigation. The presence and nature of contamination from other types of releases (such as migration and any potential releases discovered during the investigation) will be evaluated using soil samples collected from the locations most likely containing contamination, if present. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to each CAS.

Seismic activity parameters of the Finnish potential repository sites

International Nuclear Information System (INIS)

Saari, J.

2000-10-01

Posiva Oy has started a project for estimating the possible earthquake induced rock movements on the deposition holes containing canisters of spent nuclear fuel. These estimates will be made for the four investigation sites, Romuvaara, Kivetty, Olkiluoto and Haestholmen. This study deals with the current and future seismicity associated with the above mentioned sites. Seismic belts that participate the seismic behaviour of the studied sites have been identified and the magnitude-frequency distributions of these belts have been estimated. The seismic activity parameters of the sites have been deduced from the characteristics of the seismic belts in order to forecast the seismicity during the next 100,000 years. The report discusses the possible earthquakes induced by future glaciation. The seismic interpretation seems to indicate that the previous postglacial faults in Finnish Lapland have been generated in compressional environment. The orientation of the rather uniform compression has been NW-SE, which coincide with the current stress field. It seems that, although the impact of postglacial crustal rebound must have been significant, the impact of plate tectonics has been dominant. A major assumption of this study has been that future seismicity will generally resemble the current seismicity. However, when the postglacial seismicity is concerned, the magnitude-frequency distribution is likely different and the expected maximum magnitude will be higher. Maximum magnitudes of future postglacial earthquakes have been approximated by strain release examinations. Seismicity has been examined within the framework of the lineament maps, in order to associate the future significant earthquakes with active fault zones in the vicinity of the potential repository sites. (orig.)

WAG 2 remedial investigation and site investigation site-specific work plan/health and safety checklist for the ecological assessment task, Kingfisher Study

International Nuclear Information System (INIS)

Holt, V.L.; Baron, L.A.

1994-05-01

This report provides specific details and requirements for the WAG 2 remedial investigation and site investigation Ecological Assessment Task, Kingfisher Study, including information that will contribute to safe completion of the project. The report includes historical background; a site map; project organization; task descriptions and hazard evaluations; controls; and monitoring, personal protective equipment, decontamination, and medical surveillance program requirements. The report also includes descriptions of site personnel and their certifications as well as suspected WAG 2 contaminants and their characteristics. The primary objective of the WAG 2 Kingfisher Study is to assess the feasibility of using kingfishers as biological monitors of contaminants on the Oak Ridge Reservation (ORR). Kingfisher sample collection will be used to determine the levels of contaminants and degree of bioaccumulation within a common piscivorous bird feeding on contaminated fish from streams on the ORR

Site descriptive modeling as a part of site characterization in Sweden - Concluding the surface based investigations

International Nuclear Information System (INIS)

Andersson, Johan; Winberg, Anders; Skagius, Kristina; Stroem, Anders; Lindborg, Tobias

2007-01-01

The Swedish Nuclear Fuel and Waste Management Co., SKB, is currently finalizing its surface based site investigations for the final repository for spent nuclear fuel in the municipalities of Oestharmnar (the Forsmark area) and Oskarshamn (the Simpevar/Laxemar area). The investigation data are assessed into a Site Descriptive Model, constituting a synthesis of geology, rock mechanics, thermal properties, hydrogeology, hydro-geochemistry, transport properties and a surface system description. Site data constitute a wide range of different measurement results. These data both need to be checked for consistency and to be interpreted into a format more amenable for three-dimensional modeling. The three-dimensional modeling (i.e. estimating the distribution of parameter values in space) is made in a sequence where the geometrical framework is taken from the geological models and in turn used by the rock mechanics, thermal and hydrogeological modeling. These disciplines in turn are partly interrelated, and also provide feedback to the geological modeling, especially if the geological description appears unreasonable when assessed together with the other data. Procedures for assessing the uncertainties and the confidence in the modeling have been developed during the course of the site modeling. These assessments also provide key input to the completion of the site investigation program. (authors)

Report of Animal Investigation Program activities for the Baneberry Event

International Nuclear Information System (INIS)

Smith, D.D.; Black, S.C.; Giles, K.R.; Moghissi, A.A.

1975-11-01

On December 18, 1970, an underground nuclear test, conducted at the Nevada Test Site, released radioactive materials into the atmosphere with resultant on-site and off-site contamination. The Animal Investigation Program of the National Environmental Research Center, Las Vegas developed studies to document the distribution of fission and activation products in the tissue of domestic and wild animals residing within contaminated areas on and surrounding the Nevada Test Site. A study of radioiodine secretion in milk from cows at the experimental dairy farm, including urine and fecal excretion from four of them, was started about 24 hours after the venting. A grazing intake study, which utilized fistulated steers, was also carried out. The analytical data collected from these studies are presented in this report

Threatened and endangered wildlife species of the Hanford Site related to CERCLA characterization activities

Energy Technology Data Exchange (ETDEWEB)

Fitzner, R.E. [Pacific Northwest Lab., Richland, WA (United States); Weiss, S.G.; Stegen, J.A. [Westinghouse Hanford Co., Richland, WA (United States)

1994-06-01

The US Department of Energy`s (DOE) Hanford Site has been placed on the National Priorities List, which requires that it be remediated under the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) or Superfund. Potentially contaminated areas of the Hanford Site were grouped into operable units, and detailed characterization and investigation plans were formulated. The DOE Richland Operations Office requested Westinghouse Hanford Company (WHC) to conduct a biological assessment of the potential impact of these characterization activities on the threatened, endangered, and sensitive wildlife species of the Hanford Site. Additional direction for WHC compliances with wildlife protection can be found in the Environmental Compliance Manual. This document is intended to meet these requirements, in part, for the CERCLA characterization activities, as well as for other work comparable in scope. This report documents the biological assessment and describes the pertinent components of the Hanford Site as well as the planned characterization activities. Also provided are accounts of endangered, threatened, and federal candidate wildlife species on the Hanford Site and information as to how human disturbances can affect these species. Potential effects of the characterization activities are described with recommendations for mitigation measures.

Anti-nuclear activities and critics concerning nuclear power plant sites

International Nuclear Information System (INIS)

Rhee, We-Beg

2000-01-01

Korea has dynamic nuclear power expansion programs, operating 16 nuclear units producing 13710 MW in total located on 4 different sites. Last year, nuclear power supplied over 40 % of national total electricity demands. In 1998, Korean government initiated re-designation work investigating circumstance changes to rule out the unnecessary sites in consideration of a long-term power supply. Korean government has determined to expand the Ulchin site and to designate one point of Woolju county as a new candidate site, and ruled out the rest candidate sites at the end of 1998. About such a governmental measure, the two areas show different reactions. Ulchin where nuclear power plant has been operated safely for about 10 years was likely to accept the governmental determination in spite of some opposition and called for several financial supports for local development. WooIju county, however, showed a strong opposition among local environmental groups and autonomous politicians, and they presented a variety of anti-nuclear activities including demonstrations mainly at the neighbouring metropolis, Ulsan city

The Nirex Sellafield site investigation: the role of geophysical interpretation

International Nuclear Information System (INIS)

Muir Wood, R.; Woo, G.; MacMillan, G.

1992-01-01

This report reviews the methods by which geophysical data are interpreted, and used to characterize the 3-D geology of a site for potential storage of radioactive waste. The report focuses on the NIREX site investigation at Sellafield, for which geophysical observations provide a significant component of the structural geological understanding. In outlining the basic technical principles of seismic data processing and interpretation, and borehole logging, an attempt has been made to identify errors, uncertainties, and the implicit use of expert judgement. To enhance the reliability of a radiological probabilistic risk assessment, recommendations are proposed for independent use of the primary NIREX geophysical site investigation data in characterizing the site geology. These recommendations include quantitative procedures for undertaking an uncertainty audit using a combination of statistical analysis and expert judgement. (author)

Improved ethanol electrooxidation performance by shortening Pd-Ni active site distance in Pd-Ni-P nanocatalysts

Science.gov (United States)

Chen, Lin; Lu, Lilin; Zhu, Hengli; Chen, Yueguang; Huang, Yu; Li, Yadong; Wang, Leyu

2017-01-01

Incorporating oxophilic metals into noble metal-based catalysts represents an emerging strategy to improve the catalytic performance of electrocatalysts in fuel cells. However, effects of the distance between the noble metal and oxophilic metal active sites on the catalytic performance have rarely been investigated. Herein, we report on ultrasmall (~5 nm) Pd-Ni-P ternary nanoparticles for ethanol electrooxidation. The activity is improved up to 4.95 A per mgPd, which is 6.88 times higher than commercial Pd/C (0.72 A per mgPd), by shortening the distance between Pd and Ni active sites, achieved through shape transformation from Pd/Ni-P heterodimers into Pd-Ni-P nanoparticles and tuning the Ni/Pd atomic ratio to 1:1. Density functional theory calculations reveal that the improved activity and stability stems from the promoted production of free OH radicals (on Ni active sites) which facilitate the oxidative removal of carbonaceous poison and combination with CH3CO radicals on adjacent Pd active sites.

Geological and geotechnical investigations for nuclear power plants sites

International Nuclear Information System (INIS)

Alves, P.R.R.

1984-09-01

This dissertation presents a general methodology for the tasks of geological and geotechnical investigations, to be performed in the proposed sites for construction of nuclear Power Plants. In this work, items dealing with the standards applied to licensing of Nuclear Power Plants, with the selection process of sites and identification of geological and geotechnical parameters needed for the regional and local characterization of the area being studied, were incorporated. This dissertation also provides an aid to the writing of Technical Reports, which are part of the documentation an owner of a Nuclear Power Plant needs to submit to the Comissao Nacional de Energia Nuclear, to fulfill the nuclear installation licensing requirements. Moreover, this work can contribute to the planning of field and laboratory studies, needed to determine the parameters of the area under investigation, for the siting of Nuclear Power Plants. (Author) [pt

Animal Investigation Program 1973 annual report: Nevada Test Site and vicinity

International Nuclear Information System (INIS)

Smith, D.D.; Giles, K.R.; Bernhardt, D.E.

1977-05-01

Data are presented from the radioanalysis of tissues collected from cattle, deer, desert bighorn sheep, and other wildlife that resided on or near the Nevada Test Site during 1973. Routine activities and special investigations are discussed. Iodine-131 was detected in the thyroid of a Nevada Test Site mule deer. The postulated source was worldwide fallout from a nuclear detonation conducted by the People's Republic of China. Other than the naturally occurring potassium-40, cesium-137 was the only gamma-emitting radionuclide detected with any consistency in soft tissues. Nine muscle samples from the Nevada Test Site beef herd contained levels of cesium-137 ranging from 14 to 50 pCi/kilogram. Muscle from two deer contained 20 and 30 pCi/kilogram. Rabbit muscle contained 200 pCi/kilogram and muscle from a feral horse contained 40 pCi/kilogram. Tritium levels in all animal tissues sampled were at background except for animals residing at the Area 15 farm and for a feral horse. Postulated sources of these exposures are discussed. The strontium content in bones continued the downward trend observed during recent years

Quantum mechanical design of enzyme active sites.

Science.gov (United States)

Zhang, Xiyun; DeChancie, Jason; Gunaydin, Hakan; Chowdry, Arnab B; Clemente, Fernando R; Smith, Adam J T; Handel, T M; Houk, K N

2008-02-01

The design of active sites has been carried out using quantum mechanical calculations to predict the rate-determining transition state of a desired reaction in presence of the optimal arrangement of catalytic functional groups (theozyme). Eleven versatile reaction targets were chosen, including hydrolysis, dehydration, isomerization, aldol, and Diels-Alder reactions. For each of the targets, the predicted mechanism and the rate-determining transition state (TS) of the uncatalyzed reaction in water is presented. For the rate-determining TS, a catalytic site was designed using naturalistic catalytic units followed by an estimation of the rate acceleration provided by a reoptimization of the catalytic site. Finally, the geometries of the sites were compared to the X-ray structures of related natural enzymes. Recent advances in computational algorithms and power, coupled with successes in computational protein design, have provided a powerful context for undertaking such an endeavor. We propose that theozymes are excellent candidates to serve as the active site models for design processes.

Site investigations, design, construction, operation, shutdown and surveillance of repositories for low- and intermediate-level radioactive wastes in rock cavities

International Nuclear Information System (INIS)

1984-01-01

The report provides an overview and technical guidelines for considerations and for activities to be undertaken for safety assessment, site investigations, design, construction, operation, shutdown and surveillance of repositories for the disposal of low- and intermediate-level radioactive wastes in rock cavities. A generalized sequence of investigations is introduced which proceeds through region and site selection to the stage where the site is confirmed by detailed geoscientific investigations as being suitable for a waste repository. The different procedures and somewhat specific investigative needs with respect to existing mines are dealt with separately. General design, as well as specific requirements with respect to the different stages of design and construction, are dealt with. A review of activities related to the operational and post-operational stages of repositories in rock cavities is presented. The report describes in general terms the procedures related to different stages of disposal operation; also the conditions for shutdown together with essential shutdown and sealing activities and the related safety assessment requirements. Guidance is also given on the surveillance programme which will allow for inspection, testing, maintenance and security of a disposal facility during the operational phase, as well as for the post-operational stage for periods determined as necessary by the national authorities

Differential active site loop conformations mediate promiscuous activities in the lactonase SsoPox.

Directory of Open Access Journals (Sweden)

Julien Hiblot

Full Text Available Enzymes are proficient catalysts that enable fast rates of Michaelis-complex formation, the chemical step and products release. These different steps may require different conformational states of the active site that have distinct binding properties. Moreover, the conformational flexibility of the active site mediates alternative, promiscuous functions. Here we focused on the lactonase SsoPox from Sulfolobus solfataricus. SsoPox is a native lactonase endowed with promiscuous phosphotriesterase activity. We identified a position in the active site loop (W263 that governs its flexibility, and thereby affects the substrate specificity of the enzyme. We isolated two different sets of substitutions at position 263 that induce two distinct conformational sampling of the active loop and characterized the structural and kinetic effects of these substitutions. These sets of mutations selectively and distinctly mediate the improvement of the promiscuous phosphotriesterase and oxo-lactonase activities of SsoPox by increasing active-site loop flexibility. These observations corroborate the idea that conformational diversity governs enzymatic promiscuity and is a key feature of protein evolvability.

Investigation of prototypal MOFs consisting of polyhedral cages with accessible Lewis-acid sites for quinoline synthesis.

Science.gov (United States)

Gao, Wen-Yang; Leng, Kunyue; Cash, Lindsay; Chrzanowski, Matthew; Stackhouse, Chavis A; Sun, Yinyong; Ma, Shengqian

2015-03-21

A series of prototypal metal-organic frameworks (MOFs) consisting of polyhedral cages with accessible Lewis-acid sites, have been systematically investigated for Friedländer annulation reaction, a straightforward approach to synthesizing quinoline and its derivatives. Amongst them MMCF-2 demonstrates significantly enhanced catalytic activity compared with the benchmark MOFs, HKUST-1 and MOF-505, as a result of a high-density of accessible Cu(II) Lewis acid sites and large window size in the cuboctahedral cage-based nanoreactor of MMCF-2.

Expansion of access tunnels and active-site cavities influence activity of haloalkane dehalogenases in organic cosolvents.

Science.gov (United States)

Stepankova, Veronika; Khabiri, Morteza; Brezovsky, Jan; Pavelka, Antonin; Sykora, Jan; Amaro, Mariana; Minofar, Babak; Prokop, Zbynek; Hof, Martin; Ettrich, Rudiger; Chaloupkova, Radka; Damborsky, Jiri

2013-05-10

The use of enzymes for biocatalysis can be significantly enhanced by using organic cosolvents in the reaction mixtures. Selection of the cosolvent type and concentration range for an enzymatic reaction is challenging and requires extensive empirical testing. An understanding of protein-solvent interaction could provide a theoretical framework for rationalising the selection process. Here, the behaviour of three model enzymes (haloalkane dehalogenases) was investigated in the presence of three representative organic cosolvents (acetone, formamide, and isopropanol). Steady-state kinetics assays, molecular dynamics simulations, and time-resolved fluorescence spectroscopy were used to elucidate the molecular mechanisms of enzyme-solvent interactions. Cosolvent molecules entered the enzymes' access tunnels and active sites, enlarged their volumes with no change in overall protein structure, but surprisingly did not act as competitive inhibitors. At low concentrations, the cosolvents either enhanced catalysis by lowering K(0.5) and increasing k(cat), or caused enzyme inactivation by promoting substrate inhibition and decreasing k(cat). The induced activation and inhibition of the enzymes correlated with expansion of the active-site pockets and their occupancy by cosolvent molecules. The study demonstrates that quantitative analysis of the proportions of the access tunnels and active-sites occupied by organic solvent molecules provides the valuable information for rational selection of appropriate protein-solvent pair and effective cosolvent concentration. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Final work plan : environmental site investigation at Sylvan Grove, Kansas.

Energy Technology Data Exchange (ETDEWEB)

LaFreniere, L. M. (Environmental Science Division)

2012-07-15

what future CCC/USDA actions may be necessary, with the ultimate goal of achieving classification of the Sylvan Grove site at no further action status. The proposed activities are to be performed on behalf of the CCC/USDA by the Environmental Science Division of Argonne National Laboratory, a nonprofit, multidisciplinary research center operated by the UChicago Argonne, LLC, for the U.S. Department of Energy. Argonne provides technical assistance to the CCC/USDA concerning environmental site characterization and remediation at former grain storage facilities. Argonne issued a Master Work Plan (Argonne 2002) that has been approved by the KDHE. The Master Work Plan describes the general scope of all investigations at former CCC/USDA facilities in Kansas and provides guidance for these investigations. That document should be consulted for the complete details of plans for work associated with the former CCC/USDA facility at Sylvan Grove.

Geo-scientific basis for the investigation site Oberbauenstock. Volume 1

International Nuclear Information System (INIS)

Schneider, T.R.; Kappeler, S.W.

1984-12-01

The geological and hydrogeological conditions prevailing in the region of the investigation site Oberbauen Stock have been chosen as model data-set, in the frame of project Gewaehr 1985, for a type B (low and intermediate level) waste repository. The report, in its capacity as a reference report for project Gewaehr presents all available, relevant site data applicable to modeling of the site. The sources of the data are presented. Geological studies and investigations, boreholes and geophysical investigations prior to the construction of the Seelisberg tunnel (as well as all underground facilities pertaining to the said tunnel) are described. In the section devoted to geology, the stratigraphy and the tectonics of the helvetic nappes in the area of the site are described. Further, the host rock, the neighbouring formations and the local tectonics are presented. In conclusion the geological boundary conditions of the area are summarized. The section devoted to hydrogeology deals with the observations and measurements carried out at the surface and in the underground facilities. As well, the present state of knowledge concerning the water chemistry is described. The section also includes a description of the regional hydrogeology. Concerning rock mechanics a summary and discussion of all available parameters and excavation classes is presented. A description of all the experiences acquired during construction follows as a second part. The section ends with a presentation of the observations and measurements of the gas content of the host rock and other formations. A section concerning long-term behaviour concludes the report. Possible future changes of the geology and the climate are investigated. The consequences for the model site are discussed. It is concluded that a negative impact to a repository on the site is improbable on a timescale of 10 5 years. (author)

Preliminary safety evaluation, based on initial site investigation data. Planning document

International Nuclear Information System (INIS)

Hedin, Allan

2002-12-01

This report is a planning document for the preliminary safety evaluations (PSE) to be carried out at the end of the initial stage of SKBs ongoing site investigations for a deep repository for spent nuclear fuel. The main purposes of the evaluations are to determine whether earlier judgements of the suitability of the candidate area for a deep repository with respect to long-term safety holds up in the light of borehole data and to provide feed-back to continued site investigations and site specific repository design. The preliminary safety evaluations will be carried out by a safety assessment group, based on a site model, being part of a site description, provided by a site modelling group and a repository layout within that model suggested by a repository engineering group. The site model contains the geometric features of the site as well as properties of the host rock. Several alternative interpretations of the site data will likely be suggested. Also the biosphere is included in the site model. A first task for the PSE will be to compare the rock properties described in the site model to previously established criteria for a suitable host rock. This report gives an example of such a comparison. In order to provide more detailed feedback, a number of thermal, hydrological, mechanical and chemical analyses of the site will also be included in the evaluation. The selection of analyses is derived from the set of geosphere and biosphere analyses preliminarily planned for the comprehensive safety assessment named SR-SITE, which will be based on a complete site investigation. The selection is dictated primarily by the expected feedback to continued site investigations and by the availability of data after the PSE. The repository engineering group will consider several safety related factors in suggesting a repository layout: Thermal calculations will be made to determine a minimum distance between canisters avoiding canister surface temperatures above 100 deg C

Corrective Action Investigation Plan for Corrective Action Unit 573: Alpha Contaminated Sites, Nevada National Security Site, Nevada, Revision 0

Energy Technology Data Exchange (ETDEWEB)

Matthews, Patrick

2014-05-01

Corrective Action Unit (CAU) 573 is located in Area 5 of the Nevada National Security Site, which is approximately 65 miles northwest of Las Vegas, Nevada. CAU 573 is a grouping of sites where there has been a suspected release of contamination associated with non-nuclear experiments and nuclear testing. This document describes the planned investigation of CAU 573, which comprises the following corrective action sites (CASs): • 05-23-02, GMX Alpha Contaminated Area • 05-45-01, Atmospheric Test Site - Hamilton These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives.

Active chemisorption sites in functionalized ionic liquids for carbon capture.

Science.gov (United States)

Cui, Guokai; Wang, Jianji; Zhang, Suojiang

2016-07-25

Development of novel technologies for the efficient and reversible capture of CO2 is highly desired. In the last decade, CO2 capture using ionic liquids has attracted intensive attention from both academia and industry, and has been recognized as a very promising technology. Recently, a new approach has been developed for highly efficient capture of CO2 by site-containing ionic liquids through chemical interaction. This perspective review focuses on the recent advances in the chemical absorption of CO2 using site-containing ionic liquids, such as amino-based ionic liquids, azolate ionic liquids, phenolate ionic liquids, dual-functionalized ionic liquids, pyridine-containing ionic liquids and so on. Other site-containing liquid absorbents such as amine-based solutions, switchable solvents, and functionalized ionic liquid-amine blends are also investigated. Strategies have been discussed for how to activate the existent reactive sites and develop novel reactive sites by physical and chemical methods to enhance CO2 absorption capacity and reduce absorption enthalpy. The carbon capture mechanisms of these site-containing liquid absorbents are also presented. Particular attention has been paid to the latest progress in CO2 capture in multiple-site interactions by amino-free anion-functionalized ionic liquids. In the last section, future directions and prospects for carbon capture by site-containing ionic liquids are outlined.

Preliminary data report of investigations conducted at the Salmon Site, Lamar County, Mississippi

International Nuclear Information System (INIS)

1994-04-01

The US Department of Energy (DOE) conducted ecological studies at the Salmon Site (SS), Lamar County, Mississippi, from the middle of June 1992 to the end of April 1993. The studies are part of the Remedial Investigation and Feasibility Study (RI/FS) being conducted by the DOE. The RI/FS is the methodology under the Comprehensive Environmental Response, Compensation, and Liability Act of 1980, as amended by the Superfund Amendments and Reauthorization Act of 1986 (CERCLA/SARA) for evaluating hazardous waste sites on the National Priorities List (NPL). The Salmon Site is not listed on the NPL but DOE has voluntarily elected to conduct the evaluation of the SS in accordance with CERCLA/SARA. As part of the remedial investigation, baseline human health and ecological risk assessments will be conducted. These baseline risk assessments will evaluate the potential impact on human health and the environment if remedial actions are not conducted, identify locations where additional information needs to be collected, help determine whether remedial actions are necessary, and provide justification for performing remedial actions. This report describes the sampling activities conducted between February and April 1993 to aid in evaluating the possible environmental impacts at the SS tailored to the specific circumstances and conditions found there. The initial investigations included identification of the flora and fauna in and around the SS, with particular emphasis on identifying sensitive environments, endangered species and their habitats, and those species consumed by humans or found in human food chains

Investigation of the Origin of Catalytic Activity in Oxide-Supported Nanoparticle Gold

Energy Technology Data Exchange (ETDEWEB)

Harrison, Ian [Univ. of Virginia, Charlottesville, VA (United States)

2017-05-26

Since Haruta’s discovery in 1987 of the surprising catalytic activity of supported Au nanoparticles, we have seen a very large number of experimental and theoretical efforts to explain this activity and to fully understand the nature of the behavior of the responsible active sites. In 2011, we discovered that a dual catalytic site at the perimeter of ~3nm diameter Au particles supported on TiO₂ is responsible for oxidative catalytic activity. O₂ molecules bind with Au atoms and Ti4+ ions in the TiO₂ support and the weakened O-O bond dissociates at low temperatures, proceeding to produce O atoms which act as oxidizing agents for the test molecule, CO. The papers supported by DOE have built on this finding and have been concerned with two aspects of the behavior of Au/TiO₂ catalysts: (1). Mechanistic behavior of dual catalytic sites in the oxidation of organic molecules such as ethylene and acetic acid; (2). Studies of the electronic properties of the TiO₂ (110) single crystal in relation to its participation in charge transfer at the occupied dual catalytic site. A total of 20 papers have been produced through DOE support of this work. The papers combine IR spectroscopic investigations of Au/TiO₂ catalysts with surface science on the TiO₂(110) and TiO₂ nanoparticle surfaces with modern density functional modeling. The primary goals of the work were to investigate the behavior of the dual Au/Ti⁴⁺ site for the partial oxidation of alcohols to acids, the hydrogenation of aldehydes and ketones to alcohols, and the condensation of oxygenate intermediates- all processes related to the utilization of biomass in the production of useful chemical energy sources.

Corrective action investigation plan for Corrective Action Unit 342: Area 23 Mercury Fire Training Pit, Nevada Test Site, Nevada

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-03-01

This Corrective Action Investigation Plan (CAIP) has been developed in accordance with the Federal Facility Agreement and Consent Order (FFACO) that was agreed to by the US Department of Energy, Nevada Operations Office (DOE/NV); the State of Nevada Division of Environmental Protection (NDEP); and the US Department of Defense (FFACO, 1996). The CAIP is a document that provides or references all of the specific information for investigation activities associated with Corrective Action Units (CAUs) or Corrective Action Sites (CASs). According to the FFACO, CASs are sites potentially requiring corrective action(s) and may include solid waste management units or individual disposal or release sites (FFACO, 1996). Corrective Action Units consist of one or more CASs grouped together based on geography, technical similarity, or agency responsibility for the purpose of determining corrective actions. This CAIP contains the environmental sample collection objectives and the criteria for conducting site investigation activities at CAU 342, the Area 23 Mercury Fire Training Pit (FTP), which is located in Area 23 at the Nevada Test Site (NTS). The NTS is approximately 88 km (55 mi) northwest of Las Vegas, Nevada. Corrective Action Unit 342 is comprised of CAS 23-56-01. The FTP is an area approximately 100 m by 140 m (350 ft by 450 ft) located west of the town of Mercury, Nevada, which was used between approximately 1965 and 1990 to train fire-fighting personnel (REECo, 1991; Jacobson, 1991). The surface and subsurface soils in the FTP have likely been impacted by hydrocarbons and other contaminants of potential concern (COPC) associated with burn activities and training exercises in the area.

Functional properties of the two redox-active sites in yeast protein disulphide isomerase in vitro and in vivo

DEFF Research Database (Denmark)

Westphal, V; Darby, N J; Winther, Jakob R.

1999-01-01

to that of human PDI, both in rearrangement and oxidation reactions. However, while the a domain active site of the human enzyme is more active than the a'-site, the reverse is the case for yPDI. This prompted us to set up an assay to investigate whether the situation would be different with a native yeast......-site to be most important. We furthermore show that the apparent difference between in vivo and in vitro activities is not due to catalytic contributions from the other PDI homologues found in yeast....

Anoxic degradation of nitrogenous heterocyclic compounds by activated sludge and their active sites.

Science.gov (United States)

Xu, Peng; Han, Hongjun; Zhuang, Haifeng; Hou, Baolin; Jia, Shengyong; Wang, Dexin; Li, Kun; Zhao, Qian

2015-05-01

The potential for degradation of five nitrogenous heterocyclic compounds (NHCs), i.e., imidazole, pyridine, indole, quinoline, and carbazole, was investigated under anoxic conditions with acclimated activated sludge. Results showed that NHCs with initial concentration of 50 mg/L could be completely degraded within 60 hr. The degradation of five NHCs was dependent upon the chemical structures with the following sequence: imidazole>pyridine>indole>quinoline>carbazole in terms of their degradation rates. Quantitative structure-biodegradability relationship studies of the five NHCs showed that the anoxic degradation rates were correlated well with highest occupied molecular orbital. Additionally, the active sites of NHCs identified by calculation were confirmed by analysis of intermediates using gas chromatography and mass spectrometry. Copyright © 2015. Published by Elsevier B.V.

Installation restoration program site investigation. Gulfport Field Training Site, Mississippi Air National Guard Gulfport-Biloxi Regional Airport Gulfport, Mississippi. Volume 2. Final report

Energy Technology Data Exchange (ETDEWEB)

NONE

1992-12-01

Site Investigation Report, Volume: 2. A Site Investigation was performed at 3 sites at the Combat Readiness Training Center, Gulfport-Bolixi. The 3 sites investigated are the: Former Fire Training Area (Site 1), the Former JP-4 Bulk Storage Area, Mill Road (Site 2), and the Motor Pool Above-Ground Diesel Fuel Storage Tank Area (Site 3). The findings of this investigation recommended further investigation at the Fire Training Area and the JP-4 Bulk Storage Tank. At Site 3 the levels of contamination did not represent a risk to human health or the environment; therefore, no further action was recommended. Volume two of this report consisted of the following Appendixes: Site Photographs (A), Well Inventory (B), Boring Logs (C), CSL Technical Memorandum (D), Data Review and Validation (E), GPS Memorandum (F), Level C Analytical Data Summary Tables (G), Slug Test (H), Special-Status Species (I), and Representative Species of Less Mobile Fish and Wildlife (J).

Realities of site investigation

International Nuclear Information System (INIS)

Beauheim, R.; Ben Benfahel, M.; Byrum, Ch.; Fedor, F.; Geier, J.; Nys, V.; Schelkes, K.; Selroos, J.O.; Szucs, I.; Whittaker, St.

2007-01-01

During the working session, Working Group C discussed the following questions proposed by the Scientific Programme Committee of AMIGO 2: - Provide a list of concrete examples of limitations and their reasons. In addressing these issues, consider the relation between what you can measure and what you would like to describe. - Can these limitations be handled by defensible uncertainty descriptions? - What has been your experience in predicting properties/responses and then making comparisons with subsequent measurements? How much 'after-fitting' was necessary? Did the exercise contribute to validation? What did it teach you about your abilities to characterise? - What are the realities of transferability of data between sites? What can actually be transferred (data, conceptual models, evaluation procedures) and what could not? - How have the experiences on possibilities and limitations influenced your investigation programme? - How are the limits in what can be achieved factored into safety assessment and engineering? (authors)

Improved ethanol electrooxidation performance by shortening Pd–Ni active site distance in Pd–Ni–P nanocatalysts

Science.gov (United States)

Chen, Lin; Lu, Lilin; Zhu, Hengli; Chen, Yueguang; Huang, Yu; Li, Yadong; Wang, Leyu

2017-01-01

Incorporating oxophilic metals into noble metal-based catalysts represents an emerging strategy to improve the catalytic performance of electrocatalysts in fuel cells. However, effects of the distance between the noble metal and oxophilic metal active sites on the catalytic performance have rarely been investigated. Herein, we report on ultrasmall (∼5 nm) Pd–Ni–P ternary nanoparticles for ethanol electrooxidation. The activity is improved up to 4.95 A per mgPd, which is 6.88 times higher than commercial Pd/C (0.72 A per mgPd), by shortening the distance between Pd and Ni active sites, achieved through shape transformation from Pd/Ni–P heterodimers into Pd–Ni–P nanoparticles and tuning the Ni/Pd atomic ratio to 1:1. Density functional theory calculations reveal that the improved activity and stability stems from the promoted production of free OH radicals (on Ni active sites) which facilitate the oxidative removal of carbonaceous poison and combination with CH3CO radicals on adjacent Pd active sites. PMID:28071650

Ecological investigations at the Pantex Plant Site, 1992

International Nuclear Information System (INIS)

Cushing, C.E.; Mazaika, R.R.; Phillips, R.C.

1993-09-01

In 1992, Pantex requested that Pacific Northwest Laboratory (PNL) conduct a series of ecological surveys to provide baseline information for designing detailed ecological studies on the various ecosystems present at the Pantex plant site near Amarillo, Texas. To this end, PNL scientist and technicians visited the site at different times to conduct investigations and collect samples: July 6--13: birds, small mammals, general habitat assessment; August 10--14: wetland vegetation, birds, small mammals, Playa invertebrates; and September 7--11: birds, small mammals. This report presents the results of these three surveys

Salt site performance assessment activities

International Nuclear Information System (INIS)

Kircher, J.F.; Gupta, S.K.

1983-01-01

During this year the first selection of the tools (codes) for performance assessments of potential salt sites have been tentatively selected and documented; the emphasis has shifted from code development to applications. During this period prior to detailed characterization of a salt site, the focus is on bounding calculations, sensitivity and with the data available. The development and application of improved methods for sensitivity and uncertainty analysis is a focus for the coming years activities and the subject of a following paper in these proceedings. Although the assessments to date are preliminary and based on admittedly scant data, the results indicate that suitable salt sites can be identified and repository subsystems designed which will meet the established criteria for protecting the health and safety of the public. 36 references, 5 figures, 2 tables

A remote palm domain residue of RB69 DNA polymerase is critical for enzyme activity and influences the conformation of the active site.

Directory of Open Access Journals (Sweden)

Agata Jacewicz

Full Text Available Non-conserved amino acids that are far removed from the active site can sometimes have an unexpected effect on enzyme catalysis. We have investigated the effects of alanine replacement of residues distant from the active site of the replicative RB69 DNA polymerase, and identified a substitution in a weakly conserved palm residue (D714A, that renders the enzyme incapable of sustaining phage replication in vivo. D714, located several angstroms away from the active site, does not contact the DNA or the incoming dNTP, and our apoenzyme and ternary crystal structures of the Pol(D714A mutant demonstrate that D714A does not affect the overall structure of the protein. The structures reveal a conformational change of several amino acid side chains, which cascade out from the site of the substitution towards the catalytic center, substantially perturbing the geometry of the active site. Consistent with these structural observations, the mutant has a significantly reduced k pol for correct incorporation. We propose that the observed structural changes underlie the severe polymerization defect and thus D714 is a remote, non-catalytic residue that is nevertheless critical for maintaining an optimal active site conformation. This represents a striking example of an action-at-a-distance interaction.

Remedial investigation for the chemical plant area of the Weldon Spring Site

International Nuclear Information System (INIS)

1992-11-01

The US Department of Energy (DOE) is responsible for management of the Weldon Spring Site Remedial Action Project (WSSRAP) under its Environmental Restoration and Waste Management Program. Major goals include eliminating potential public and environmental hazards due to site contamination and releasing the property for alternate uses to the maximum extent practicable. The purpose of the remedial investigation described in this report was to determine the extent of contamination associated with the portion of the Weldon Spring site known as the chemical plant and raffinate pit area. The DOE has assumed responsibility for investigating and remediating all on-site soil contamination and off-site soil which is radiologically contaminated as a result of uranium and thorium processing operations. The DOE has also assumed the responsibility for radiologically contaminated groundwater on and off site. The Weldon Spring site remedial investigation also involved the evaluation of the sources, nature and extent, and environmental fate and transport of contaminants to provide a basis for defining the risks that the contaminants may pose to human health and the environment. Data are included in this report to support the screening of remedial technologies and to permit the development and detailed analysis of alternatives for remedial action at the site during the feasibility study process

A Hypothesis-Driven Approach to Site Investigation

Science.gov (United States)

Nowak, W.

2008-12-01

Variability of subsurface formations and the scarcity of data lead to the notion of aquifer parameters as geostatistical random variables. Given an information need and limited resources for field campaigns, site investigation is often put into the context of optimal design. In optimal design, the types, numbers and positions of samples are optimized under case-specific objectives to meet the information needs. Past studies feature optimal data worth (balancing maximum financial profit in an engineering task versus the cost of additional sampling), or aim at a minimum prediction uncertainty of stochastic models for a prescribed investigation budget. Recent studies also account for other sources of uncertainty outside the hydrogeological range, such as uncertain toxicity, ingestion and behavioral parameters of the affected population when predicting the human health risk from groundwater contaminations. The current study looks at optimal site investigation from a new angle. Answering a yes/no question under uncertainty directly requires recasting the original question as a hypothesis test. Otherwise, false confidence in the resulting answer would be pretended. A straightforward example is whether a recent contaminant spill will cause contaminant concentrations in excess of a legal limit at a nearby drinking water well. This question can only be answered down to a specified chance of error, i.e., based on the significance level used in hypothesis tests. Optimal design is placed into the hypothesis-driven context by using the chance of providing a false yes/no answer as new criterion to be minimized. Different configurations apply for one-sided and two-sided hypothesis tests. If a false answer entails financial liability, the hypothesis-driven context can be re-cast in the context of data worth. The remaining difference is that failure is a hard constraint in the data worth context versus a monetary punishment term in the hypothesis-driven context. The basic principle

Corrective Action Investigation Plan for Corrective Action Unit 366: Area 11 Plutonium Valley Dispersion Sites, Nevada National Security Site, Nevada, Revision 0

Energy Technology Data Exchange (ETDEWEB)

Patrick Matthews

2011-09-01

associated with the nuclear tests, it was determined that CASs 11-23-02, 11-23-03, and 11-23-04 will be investigated as one release site. The three test areas associated with these CASs are in close proximity; the devices tested were all composed of plutonium and enriched uranium; and the ground zeroes are all posted high contamination areas (HCAs). Because the device tested at CAS 11-23-01 was composed primarily of enriched uranium and the ground zero is not a posted HCA, the CAS will be investigated as a separate release. The DQO process also resulted in an assumption that TED within the HCAs and contaminated waste dumps exceeds the FAL and requires corrective action. A field investigation will be performed to define where TED exceeds the FAL and to determine whether other contaminants of concern are present at the site associated with other activities that took place at the site or from spills or waste discovered during the investigation. The presence and nature of contamination from other types of releases (such as migration and any potential releases discovered during the investigation) will be evaluated using soil samples collected from the locations most likely containing contamination, if present. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to each CAS.

Corrective Action Investigation Plan for Corrective Action Unit 321: Area 22 Weather Station Fuel Storage, Nevada Test Site, Nevada; TOPICAL

International Nuclear Information System (INIS)

1999-01-01

This Corrective Action Investigation Plan (CAIP) has been developed in accordance with the Federal Facility Agreement and Consent Order (FFACO) that was agreed to by the US Department of Energy, Nevada Operations Office (DOE/NV); the State of Nevada Division of Environmental Protection (NDEP); and the US Department of Defense (FFACO, 1996). The CAIP is a document that provides or references all of the specific information for investigation activities associated with Corrective Action Units (CAUs) or Corrective Action Sites (CASs). According to the FFACO (1996), CASs are sites potentially requiring corrective action(s) and may include solid waste management units or individual disposal or release sites. A CAU consists of one or more CASs grouped together based on geography, technical similarity, or agency responsibility for the purpose of determining corrective actions. This CAIP contains the environmental sample collection objectives and the criteria for conducting site investigation activities at the CAU 321 Area 22 Weather Station Fuel Storage, CAS 22-99-05 Fuel Storage Area. For purposes of this discussion, this site will be referred to as either CAU 321 or the Fuel Storage Area. The Fuel Storage Area is located in Area 22 of the Nevada Test Site (NTS). The NTS is approximately 105 kilometers (km) (65 miles[mi]) northwest of Las Vegas, Nevada (Figure 1-1) (DOE/NV, 1996a). The Fuel Storage Area (Figure 1-2) was used to store fuel and other petroleum products necessary for motorized operations at the historic Camp Desert Rock facility which was operational from 1951 to 1958 at the Nevada Test Site, Nevada. The site was dismantled after 1958 (DOE/NV, 1996a)

Verification study on technology for site investigation for geological disposal. Confirmation of the applicability of survey methods through establishing site descriptive models in accordance with stepwise investigation approach

International Nuclear Information System (INIS)

Kondo, Hirofumi; Suzuki, Koichi; Hasegawa, Takuma; Hamada, Takaomi; Yoshimura, Kimitaka

2014-01-01

The Yokosuka Demonstration and Validation Project, which uses the Yokosuka Central Research Institute of Electric Power Industry (CRIEPI) site, a Neogene sedimentary and coastal environment, has been conducted since the 2006 fiscal year as a cooperative research project between NUMO (Nuclear Waste Management Organization of Japan) and CRIEPI. The objectives of this project were to examine and refine the basic methodology of the investigation and assessment in accordance with the conditions of geological environment at each stage of investigations from the surface (Preliminary Investigation and the first half of Detailed Investigation conducted by NUMO) for high level radioactive waste geological disposal. Within investigation technologies at these early stages, a borehole survey is an important means of directly obtaining various properties of the deep geological environment. On the other hand, surface geophysical prospecting data provide information about the geological and resistivity structures at depth for planning borehole surveys. During the 2006-2009 fiscal years, a series of on-site surveys and tests, including borehole surveys of YDP-1 (depth: 350 m) and YDP-2 (depth: 500 m), were conducted in this test site. Furthermore, seismic surveys (including seismic reflection method) and electromagnetic surveys (including magnetotelluric method) were conducted within the expanded CRIEPI site in the 2010 fiscal year to obtain information about the geological structure, and the resistivity structure reflecting the distribution of the salt water/fresh water boundary, respectively, to a depth of over several hundred meters. The validity of existing survey and testing methods for stepwise investigations (from surface to borehole surveys) for obtaining properties of the geological environment (in various conditions relating to differences in the properties of the Miura and the Hayama Groups at this site) was confirmed through establishing site descriptive models based on

From 3D to 2D Co and Ni Oxyhydroxide Catalysts: Elucidation of the Active Site and Influence of Doping on the Oxygen Evolution Activity

DEFF Research Database (Denmark)

Tripkovic, Vladimir; Hansen, Heine Anton; Vegge, Tejs

2017-01-01

Layered oxyhydroxides (ox-hys) of Ni and Co are among the most active catalysts for oxygen evolution in alkaline media. Their activities can be further tuned by delamination into single-layer oxide sheets or by means of doping. The active site for the reaction and how doping and delamination...... investigate the role of terrace and edge sites and use stability, catalytic activity, and electronic conductivity as evaluation criteria to pinpoint the best catalysts. We arrive at several important conclusions: the ox-hy surface is fully oxidized under oxygen evolution conditions, bulk terraces...

Geological, geophysical investigations and seismotectonic analysis with reference to selection of site for nuclear power plants: a review

International Nuclear Information System (INIS)

Chaki, Anjan

2014-01-01

Geological, geophysical investigations and seismotectonic analysis play a major role in qualifying a proposed site for establishment of nuclear power plants. In an area, it is important to understand the aspects such as regional and local geology, geomorphology, tectonic settings, presence of active faults/capable faults, earthquake history and earthquake proneness, neotectonic activity, slope instability, subsidence, liquefaction, seismically induced flooding, tsunami and geohydrological conditions. Geological investigations comprise use of remote sensing and ground validation followed by geological mapping, identification of faults, near surface geological studies for foundation conditions, stratigraphic drilling, palaeoseismology, studies on engineering properties of rock and soil. Geophysical investigations provide insight into subsurface geology including concealed faults, elastic constants and hydrological conditions. Radon emanometry is a valuable tool in the initial stage to decipher subsurface active weak zones/fault lines. Seismotectonic analysis identifies the provinces of tectonic significance and their earthquake potential, thereby designating lineaments of consequence leading to their evaluation. This, in turn, determines the design basis earthquake parameter for the estimation of vibratory ground motion. This article provides certain measures to evaluate the suitability of the sites for the establishment of nuclear power plants in terms of geological, geophysical investigations and seismotectonic status. Atomic Minerals Directorate for Exploration and Research (AMD) had carried out seismotectonic analysis of the area around Kaiga, Narora, Kalpakkam, Kakrapar, Tarapur, Kudankulam and Rawatbhata Nuclear Power Projects, which were either in operation or under expansion and construction. Such analysis was extended to a number of proposed sites for establishing nuclear power plants in West Bengal, Bihar, Orissa, Andhra Pradesh, Gujrat, Madhya Pradesh

Direct instrumental identification of catalytically active surface sites

Science.gov (United States)

Pfisterer, Jonas H. K.; Liang, Yunchang; Schneider, Oliver; Bandarenka, Aliaksandr S.

2017-09-01

The activity of heterogeneous catalysts—which are involved in some 80 per cent of processes in the chemical and energy industries—is determined by the electronic structure of specific surface sites that offer optimal binding of reaction intermediates. Directly identifying and monitoring these sites during a reaction should therefore provide insight that might aid the targeted development of heterogeneous catalysts and electrocatalysts (those that participate in electrochemical reactions) for practical applications. The invention of the scanning tunnelling microscope (STM) and the electrochemical STM promised to deliver such imaging capabilities, and both have indeed contributed greatly to our atomistic understanding of heterogeneous catalysis. But although the STM has been used to probe and initiate surface reactions, and has even enabled local measurements of reactivity in some systems, it is not generally thought to be suited to the direct identification of catalytically active surface sites under reaction conditions. Here we demonstrate, however, that common STMs can readily map the catalytic activity of surfaces with high spatial resolution: we show that by monitoring relative changes in the tunnelling current noise, active sites can be distinguished in an almost quantitative fashion according to their ability to catalyse the hydrogen-evolution reaction or the oxygen-reduction reaction. These data allow us to evaluate directly the importance and relative contribution to overall catalyst activity of different defects and sites at the boundaries between two materials. With its ability to deliver such information and its ready applicability to different systems, we anticipate that our method will aid the rational design of heterogeneous catalysts.

Investigation of tritium in groundwater at Site 300

International Nuclear Information System (INIS)

Buddemeier, R.W.

1985-01-01

In 1984, landfill monitoring wells at Site 300, a Lawrence Livermore National Laboratory (LLNL) explosive test site, revealed the presence of groundwater contaminated with tritium. These tritium levels were in excess of the State of California drinking water standard. A major investigation was initiated that included a search of records concerning tritium use, disposal, and previous analyses, and a survey of tritium levels in soil, vegetation, and water in contaminated and potentially contaminated areas. Over 50 boreholes were drilled for this investigation to characterize the local hydrogeology and tritium distributions, and a network of soil moisture and groundwater monitoring points was installed. This report presents the work completed through the end of September 1985: the records search; records for drilling completed as part of this study; characterization of the geology, hydrology, and tritium distributions in the contaminated area; and an initial assessment of the probable tritium sources, pathways, and migration rates. 19 refs

Investigation of tritium in groundwater at Site 300

Energy Technology Data Exchange (ETDEWEB)

Buddemeier, R.W.

1985-12-30

In 1984, landfill monitoring wells at Site 300, a Lawrence Livermore National Laboratory (LLNL) explosive test site, revealed the presence of groundwater contaminated with tritium. These tritium levels were in excess of the State of California drinking water standard. A major investigation was initiated that included a search of records concerning tritium use, disposal, and previous analyses, and a survey of tritium levels in soil, vegetation, and water in contaminated and potentially contaminated areas. Over 50 boreholes were drilled for this investigation to characterize the local hydrogeology and tritium distributions, and a network of soil moisture and groundwater monitoring points was installed. This report presents the work completed through the end of September 1985: the records search; records for drilling completed as part of this study; characterization of the geology, hydrology, and tritium distributions in the contaminated area; and an initial assessment of the probable tritium sources, pathways, and migration rates. 19 refs.

Site characterization investigations at Oak Ridge National Laboratory

International Nuclear Information System (INIS)

Ketelle, R.H.

1985-01-01

The geologic and geohydrologic characterization and assessment techniques currently used at ORNL are integrated into a systematic approach. The investigations are multi-faceted, and involve investigators with a variety of expertise. Characterization studies are designed to obtain the data requirements of pathways analysis and facility design in addition to the detailed site description. The approach effectively minimizes the redundancy and lack of coordination which often arise when the study is broken down into totally independent tasks. The geologic environment of the Oak Ridge Reservation is one of structural and stratigraphic complexity which requires a comprehensive and systematic approach to characterize. Recent characterization studies have included state-of-the-science techniques in the areas of unsaturated zone testing, geochemical tests to determine attenuation properties of soils, and numerical analyses of site performance. The results of these studies and analyses are changing the technology of shallow land burial by indicating that chemically stable waste forms are required to limit radionuclide migration to acceptable levels. 11 refs., 1 tab

Different integrated geophysical approaches to investigate archaeological sites in urban and suburban area.

Science.gov (United States)

Piro, Salvatore; Papale, Enrico; Zamuner, Daniela

2016-04-01

Geophysical methods are frequently used in archaeological prospection in order to provide detailed information about the presence of structures in the subsurface as well as their position and their geometrical reconstruction, by measuring variations of some physical properties. Often, due to the limited size and depth of an archaeological structure, it may be rather difficult to single out its position and extent because of the generally low signal-to-noise ratio. This problem can be overcome by improving data acquisition, processing techniques and by integrating different geophysical methods. In this work, two sites of archaeological interest, were investigated employing several methods (Ground Penetrating Radar (GPR), Electrical Resistivity Tomography (ERT), Fluxgate Differential Magnetic) to obtain precise and detailed maps of subsurface bodies. The first site, situated in a suburban area between Itri and Fondi, in the Aurunci Natural Regional Park (Central Italy), is characterized by the presence of remains of past human activity dating from the third century B.C. The second site, is instead situated in an urban area in the city of Rome (Basilica di Santa Balbina), where historical evidence is also present. The methods employed, allowed to determine the position and the geometry of some structures in the subsurface related to this past human activity. To have a better understanding of the subsurface, we then performed a qualitative and quantitative integration of this data, which consists in fusing the data from all the methods used, to have a complete visualization of the investigated area. Qualitative integration consists in graphically overlaying the maps obtained by the single methods; this method yields only images, not new data that may be subsequently analyzed. Quantitative integration is instead performed by mathematical and statistical solutions, which allows to have a more accurate reconstruction of the subsurface and generates new data with high

Site characterization and validation - hydrochemical investigations. Stage 3

International Nuclear Information System (INIS)

Laaksoharju, M.

1990-02-01

The objective for the Stage 3 hydrochemical investigations was to classify groundwater and to determine the different flow paths within the investigated SCV-site by using water analyses from the C and D boreholes. The water was divided into three classes shallow (A), mixed (B) and deep groundwater (C) based on Cl and HCO 3 concentration. The local geohydrological situation in the SCV-site can be divided into a disturbed situation and an undisturbed situation. Opening of the boreholes and smapling causes a disturbance of hydrochemical conditions. Three water types were found in the important water conductors, the GB and the GH zones. Shallow water (A-type) is flowing downwards while deep groundwater (C-type) is flowing upwards driven by the pumping of the mine. Where the two water types meet a zone of approximately 30 m thickness with mixed (B-type) water is formed. The flow situation is revealed by the geohydrological measurements. At undisturbed conditions shallow water (A-type) is flowing down in the investigated zones. The B and C water types are then found at a deeper level than during disturbed conditions. A regional model can be constructed based on the described chemical and geohydrological investigations. Shallow water from the top and deep groundwater from below are drawn towards the mine by the pumping. Where these waters meet mixed water is formed. (orig./HP)

Active sites environmental monitoring Program - Program Plan: Revision 2

International Nuclear Information System (INIS)

Morrissey, C.M.; Hicks, D.S.; Ashwood, T.L.; Cunningham, G.R.

1994-05-01

The Active Sites Environmental Monitoring Program (ASEMP), initiated in 1989, provides early detection and performance monitoring of active low-level-waste (LLW) and transuranic (TRU) waste facilities at Oak Ridge National Laboratory (ORNL). Several changes have recently occurred in regard to the sites that are currently used for waste storage and disposal. These changes require a second set of revisions to the ASEMP program plan. This document incorporates those revisions. This program plan presents the organization and procedures for monitoring the active sites. The program plan also provides internal reporting levels to guide the evaluation of monitoring results

Corrective Action Investigation Plan for Corrective Action Unit 551: Area 12 Muckpiles, Nevada Test Site, Nevada

International Nuclear Information System (INIS)

Boehlecke, Robert F.

2004-01-01

This Corrective Action Investigation Plan (CAIP) contains project-specific information including facility descriptions, environmental sample collection objectives, and criteria for conducting site investigation activities at Corrective Action Unit (CAU) 551, Area 12 muckpiles, Nevada Test Site (NTS), Nevada. This CAIP has been developed in accordance with the 'Federal Facility Agreement and Consent Order' (FFACO) (1996) that was agreed to by the State of Nevada, the U.S. Department of Energy (DOE), and the U.S. Department of Defense. Corrective Action Unit 551 is located in Area 12 of the NTS, which is approximately 110 miles (mi) northwest of Las Vegas, Nevada (Figure 1-1). Area 12 is approximately 40 miles beyond the main gate to the NTS. Corrective Action Unit 551 is comprised of the four Corrective Action Sites (CASs) shown on Figure 1-1 and listed below: (1) 12-01-09, Aboveground Storage Tank and Stain; (2) 12-06-05, Muckpile; (3) 12-06-07, Muckpile; and (4) 12-06-08, Muckpile. Corrective Action Site 12-01-09 is located in Area 12 and consists of an above ground storage tank (AST) and associated stain. Corrective Action Site 12-06-05 is located in Area 12 and consists of a muckpile associated with the U12 B-Tunnel. Corrective Action Site 12-06-07 is located in Area 12 and consists of a muckpile associated with the U12 C-, D-, and F-Tunnels. Corrective Action Site 12-06-08 is located in Area 12 and consists of a muckpile associated with the U12 B-Tunnel. In keeping with common convention, the U12B-, C-, D-, and F-Tunnels will be referred to as the B-, C-, D-, and F-Tunnels. The corrective action investigation (CAI) will include field inspections, radiological surveys, and sampling of media, where appropriate. Data will also be obtained to support waste management decisions

Integrated experiment activity monitoring for wLCG sites based on GWT

International Nuclear Information System (INIS)

Feijóo, Alejandro Guinó; Espinal, Xavier

2011-01-01

The goal of this work is to develop a High Level Monitoring (HLM) where to merge the distributed computing activities of an LHC experiment (ATLAS). ATLAS distributed computing is organized in clouds, where the Tier-Is (primary centers) provide services to the associated Tier-2s centers (secondaries) so they are all seen as a cloud by the experiment. Computing activities and sites stability monitoring services are numerous and delocalized. It would be very useful for a cloud manager to have a single place where to aggregate available monitoring information. The idea presented in this paper is to develop a set of collectors to gather information regarding site status and performance on data distribution, data processing and Worldwide LHC Computing Grid (WLCG) tests (Service Availability Monitoring), store them in specific databases, process the results and show it in a single HLM page. Once having it, one can investigate further by interacting with the front-end, which is fed by the stats stored on databases.

Prioritization to limit sampling and drilling in site investigations

International Nuclear Information System (INIS)

Burton, J.C.

1992-01-01

One of the major goals of the Environmental Research Division of Argonne National Laboratory is to develop and provide governmental agencies with technically sound, cost-effective frameworks for environmental site characterization and remedial programs. An example of the development of such a framework for preremedial site characterization is presented in this paper. Specifically, this paper presents portions of an expanded site investigation program developed for landfills suspected of containing hazardous waste. The work was sponsored by the New Mexico State Office of the US Department of Interior's Bureau of Land Management (BLM). The emphasis of the BLM program was on identifying initial characterization procedures that would decrease the need for sampling and drilling on a random grid

Investigation of nonlinear dynamic soil property at the Savannah River Site

International Nuclear Information System (INIS)

Lee, R.C.

2000-01-01

This document summarizes laboratory dynamic soil testing investigations conducted by the University of Texas at Austin (UTA) for the Savannah River Site (SRS) (Stokoe et al., 1995a, Stokoe et al., 1995b, Sponseller and Stokoe, 1995). The purpose of the investigation is to provide an evaluation of past testing results in the context of new test data and the development of consistent site wide models of material strain dependencies based upon geologic formation, depth, and relevant index properties

Hydrogeochemical investigation of six geothermal sites in Honduras, Central America

Energy Technology Data Exchange (ETDEWEB)

Goff, F.E.; Truesdell, A.H.; Grigsby, C.O.; Janik, C.J.; Shevenell, L.A.; Paredes, J.R.; Gutierrez, J.W.; Trujillo, Jr.; Counce, D.A.

1987-06-01

We conducted detailed hydrogeochemical investigations at six geothermal sites in western Honduras: Azacualpa, El Olivar, Pavana, Platanares, Sambo Creek, and San Ignacio. None of the sites is associated with Quaternary silicic volcanism, although El Olivar lies adjacent to a small Quaternary basalt field and Pavana is part of a belt of hot spring activity parallel to and 35 km east of the Central American volcanic arc. None of the sites contains acid-sulfate waters indicative of vapor-dominated conditions. Thermal fluids are characterized by pH between 7 and 10, Cl<125 mg/l, HCO/sub 3/>Cl, SO/sub 4/greater than or equal toCl, Bless than or equal to17 mg/l, Liless than or equal to4 mg/l, and Asless than or equal to1.25 mg/l. Stable isotope analyses of the water show that recharge to the geothermal systems generally occurs from areas of higher elevation adjacent to the sites. Tritium contents of apparently undiluted thermal fluids range from 0 to 0.4 T.U., indicating residence times of fluids in the systems of more than 500 y. Various geochemical indicators show that mixing of hot and cold end-member fluids occurs in the system at Platanares and, to a lesser degree, in the systems at San Ignacio and Azacualpa. No mixing is apparent in the fluids discharging at Pavana, Sambo Creek, or El Olivar. Boiling is the dominant process responsible for subtle geochemical variations at Azacualpa and, possibly, San Ignacio. Our best estimates of subsurface reservoir temperatures are 225/sup 0/C at Platanares, 190/sup 0/C at San Ignacio, 185/sup 0/C at Azacualpa, 155/sup 0/C at Sambo Creek, 150/sup 0/C at Pavana, and 120/sup 0/C at El Olivar. The estimated power output of the three hottest sites is 45 thermal megawatts at Platanares, 14 thermal megawatts at San Ignacio, and 13 thermal megawatts at Azacualpa.

Surface radiological investigations at the 0816 Site, Waste Area Grouping 13, Oak Ridge National Laboratory, Oak Ridge, Tennessee

International Nuclear Information System (INIS)

Tiner, P.F.; Uziel, M.S.

1994-12-01

A surface radiological investigation was conducted intermittently from July through September 1994 at the 0816 site, located within Waste Area Grouping (WAG) 13. The survey was performed by members of the Measurement Applications and Development Group, Health Sciences Research Division, Oak Ridge National Laboratory (ORNL) at the request of ORNL Site Environmental Restoration Program Facility Management. The purpose of the survey was to ascertain and document the surface radiological condition of the site subsequent to remedial action activities completed in May 1994. The survey was designed to determine whether any residual surface sod contamination in excess of 120 pCi/g 137 Cs (Specified by the Interim Record of Decision) remained at the site

Probing the active sites for CO dissociation on ruthenium nanoparticles

DEFF Research Database (Denmark)

Strebel, Christian Ejersbo; Murphy, Shane; Nielsen, Rasmus Munksgård

2012-01-01

affect the CO dissociation activity. The Ru nanoparticles were synthesized in a UHV chamber by gas-aggregation magnetron sputtering in the size range from 3 to 15 nm and the morphology was investigated in situ by scanning tunneling microscopy and ex situ by high resolution transmission electron...... microscopy. Surprisingly, it was found that larger particles were more active per surface area for CO dissociation. It is suggested that this is due to larger particles exposing a more rough surface than the smaller particles, giving rise to a higher relative amount of under-coordinated adsorption sites...... on the larger particles. The induced surface roughness is proposed to be a consequence of the growth processes in the gas-aggregation chamber....

An investigation of site diversity and comparison with ITU-R recommendations

Science.gov (United States)

Callaghan, S. A.; Boyes, B.; Couchman, A.; Waight, J.; Walden, C. J.; Ventouras, S.

2008-08-01

Earth-space radio systems operating at frequencies of 10 GHz and above are badly attenuated by rain, cloud, and atmospheric gases. As the frequencies of operational systems increase, it becomes increasingly uneconomic to compensate for the effects of fading through the use of a fixed fade margin, hence the implementation of fade mitigation techniques (FMT). The spatial and temporal variation of rain provides the justification for the use of site diversity as an FMT. Site diversity employs two or more ground stations receiving the same satellite signal with a separation distance such that the sites encounter intense rainfall at different times, and switching to the site experiencing the least fading improves system performance considerably. Measurements of the 20.7 GHz beacon carried as part of the Global Broadcast Service (GBS) payload on the U.S. Department of Defense satellite UFO-9 have been made at three sites: two are located in the South of England (˜8 km apart), and the third receiver was located in Scotland. These beacon measurements have produced long term attenuation exceedance and site diversity gain and improvement statistics. This attenuation time series data can simulate the performance of an Earth-space system using site diversity, indicating the optimum method of implementing this FMT. In this paper, unbalanced site diversity is investigated, as this is a more likely scenario than the balanced site diversity modeled by the ITU-R recommendations. This paper also investigates the implementation of site diversity from a commercial context, including cost-benefit analysis and technical feasibility.

Animal investigation program 1975 annual report: Nevada Test Site and vicinity

International Nuclear Information System (INIS)

Smith, D.D.; Giles, K.R.; Bernhardt, D.E.; Brown, K.W.

1978-02-01

Data are presented from the radioanalysis of tissues collected from cattle, deer, desert bighorn sheep, and other wildlife that resided on or near the Nevada Test Site during 1975. Routine activities and special investigations of the Animal Investigation Program are also discussed. Other than the naturally occurring potassium-40, gamma-emitting radionuclides are detected infrequently. Tritium concentrations in the tissues from most of the animals sampled were at background levels. Strontium-90 levels in bones from deer and cattle were slightly lower than those reported for the preceding year while levels in desert bighorn sheep bones were elevated. A graph depicts the average levels found in the bones of the three species from 1956 through 1975. The gross and microscopic lesions found in necropsied animals are discussed. In general, these lesions are consistent with the physical condition of the animal and type of population sampled. No gross or microscopic lesions were detected that could be directly attributed to the effects of ionizing radiation

Widely available active sites on Ni2P for electrochemical hydrogen evolution - insights from first principles calculations

DEFF Research Database (Denmark)

Hansen, Martin Hangaard; Stern, Lucas-Alexandre; Feng, Ligang

2015-01-01

We present insights into the mechanism and the active site for hydrogen evolution on nickel phosphide (Ni2P). Ni2P was recently discovered to be a very active non-precious hydrogen evolution catalyst. Current literature attributes the activity of Ni2P to a particular site on the (0001) facet....... In the present study, using Density Functional Theory (DFT) calculations, we show that several widely available low index crystal facets on Ni2P have better properties for a high catalytic activity. DFT calculations were used to identify moderately bonding nickel bridge sites and nickel hollow sites for hydrogen...... adsorption and to calculate barriers for the Tafel pathway. The investigated surfaces in this study were the (10 (1) over bar0), ((1) over bar(1) over bar 20), (11 (2) over bar0), (11 (2) over bar1) and (0001) facets of the hexagonal Ni2P crystal. In addition to the DFT results, we present experiments on Ni2...

Work plan for the remedial investigation/feasibility study-environmental assessment for the Colonie site, Colonie, New York

International Nuclear Information System (INIS)

1990-06-01

This work plan has been prepared to document the scoping and planning process performed by the US Department of Energy (DOE) to support remedial action activities at the Colonie site. The site is located in eastern New York State in the town of Colonie near the city of Albany. Remedial action of the Colonie site is being planned as part of DOE's Formerly Utilized Sites Remedial Action Program. The DOE is responsible for controlling the release of all radioactive and chemical contaminants from the site. Under the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA), a remedial investigation/feasibility study (RI/FS) must be prepared to support the decision-making process for evaluating remedial action alternatives. This work plan contains a summary of information known about the site as of January 1988, presents a conceptual site model that identifies potential routes of human exposure to site containments, identifies data gaps, and summarizes the process and proposed studies that will be used to fill the data gaps. In addition, DOE activities must be conducted in compliance with the National Environmental Policy Act (NEPA), which requires consideration of the environmental consequences of a proposed action as part of its decision-making process. This work also describes the approach that will be used to evaluate potential remedial action alternatives and includes a description of the organization, project controls, and task schedules that will be employed to fulfill the requirements of both CERCLA and NEPA. 48 refs., 18 figs., 25 tabs

Geological status of NWTS repository siting activities in the paradox basin

International Nuclear Information System (INIS)

Frazier, N.A.; Conwell, F.R.

1981-01-01

Emplacement of waste packages in mined geological repositories is one method being evaluated for isolating high-level nuclear wastes. Granite, dome salt, tuff, basalt and bedded salt are among the rock types being investigated. Described in this paper is the status of geological activities in the Paradox Basin of Utah and Colorado, one region being explored as a part of the National Waste Terminal Storage (NWTS) program to site a geological repository in bedded salt

Bald eagle site management plan for the Hanford Site, south-central Washington

International Nuclear Information System (INIS)

Fitzner, R.F.; Weiss, S.G.

1994-12-01

The CERCLA remedial investigations of waste sites on the Hanford Site will involve lands containing or adjacent to a bald eagle nest, winter concentration areas, or communal night roost. Because these CERCLA investigations may affect bald eagles, the DOE has prepared this Bald Eagle Site Management Plan (BESMP). However, it is intended that this BESMP be used or updated so as to be also applicable to future activities that affect bald eagles on the Hanford Site. Bald eagles regularly use the US Department of Energy's (DOE) Hanford Site in south-central Washington State during winter months for roosting, perching, and foraging. Each of these activities requires buffer zones to protect eagles from human disturbances. Buffer zones developed in this plan follow recommended guidelines and are intended to be used in planning. If Hanford Site activities in the vicinity of identified bald eagle use areas are carried out in accordance with this plan, such actions are not likely to adversely affect the eagles or their habitat. Activities that may be exceptions will involve informal or formal (whichever is appropriate) consultation with the US Fish and Wildlife Service as required by the Endangered Species Act

Corrective Action Investigation Plan for Corrective Action Unit 487: Thunderwell Site, Tonopah Test Range, Nevada (Rev. No.: 0, January 2001)

Energy Technology Data Exchange (ETDEWEB)

DOE/NV

2001-01-02

This Corrective Action Investigation Plan contains the U.S. Department of Energy, Nevada Operations Office's (DOE/NV's) approach to collect the data necessary to evaluate corrective action alternatives (CAAs) appropriate for the closure of Corrective Action Unit (CAU) 487, Thunderwell Site, Tonopah Test Range (TTR), Nevada, under the Federal Facility Agreement and Consent Order. Corrective Action Unit 487 consists of a single Corrective Action Site (CAS), RG 26-001-RGRV, Thunderwell Site. The site is located in the northwest portion of the TTR, Nevada, approximately five miles northwest of the Area 3 Control Point and closest to the Cactus Flats broad basin. Historically, Sandia National Laboratories in New Mexico used CAU 487 in the early to mid-1960s for a series of high explosive tests detonated at the bottom of large cylindrical steel tubes. Historical photographs indicate that debris from these tests and subsequent operations may have been scattered and buried throughout the site. A March 2000 walk-over survey and a July 2000 geophysical survey indicated evidence of buried and surface debris in dirt mounds and areas throughout the site; however, a radiological drive-over survey also performed in July 2000 indicated that no radiological hazards were identified at this site. Based on site history, the scope of this plan is to resolve the problem statement identified during the Data Quality Objectives process that detonation activities at this CAU site may have resulted in the release of contaminants of concern into the surface/subsurface soil including total volatile and total semivolatile organic compounds, total Resource Conservation and Recovery Act metals, radionuclides, total petroleum hydrocarbons, and high explosives. Therefore, the scope of corrective action field investigation will involve excavation, drilling, and extensive soil sampling and analysis activities to determine the extent (if any) of both the lateral and vertical contamination

New geochemical investigations in Platanares and Azacualpa geothermal sites (Honduras)

Science.gov (United States)

Barberi, Franco; Carapezza, Maria Luisa; Cioni, Roberto; Lelli, Matteo; Menichini, Matia; Ranaldi, Massimo; Ricci, Tullio; Tarchini, Luca

2013-05-01

Platanares and Azacualpa geothermal sites of Honduras are located in an inner part of the Caribbean Plate far from the active volcanic front of Central America. Here geology indicates that there are not the conditions for the occurrence of shallow magmatic heat sources for high-enthalpy geothermal resources. Geothermal perspectives are related to the possibility of a deep circulation of meteoric water along faults and the storage of the heated fluid in fractured permeable reservoirs. Geochemical geothermometers indicate a temperature for the deeper part of the geothermal reservoir close to 200 °C for Platanares and of 150-170 °C for Azacualpa. Calcite scaling, with subordinate silica deposition has to be expected in both sites. CO2 soil flux investigations have been carried out in both areas and reveal the presence of positive anomalies likely corresponding to the presence at depth of fractured degassing geothermal reservoirs. Compared with the geothermal areas of Central Italy whose reservoirs are hosted in carbonate rocks, e.g. Latera (Chiodini et al., 2007), the CO2 soil flux measured in Honduras is significantly lower (mean of 17 g/m2day at Platanares and of 163 g/m2day at Azacualpa) probably because of the dominant silicate nature of the deep reservoirs.

Corrective Action Investigation Plan for Corrective Action Unit 139: Waste Disposal Sites, Nevada Test Site, Nevada, Rev. No.: 0

Energy Technology Data Exchange (ETDEWEB)

Grant Evenson

2006-04-01

Corrective Action Unit (CAU) 139 is located in Areas 3, 4, 6, and 9 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 139 is comprised of the seven corrective action sites (CASs) listed below: (1) 03-35-01, Burn Pit; (2) 04-08-02, Waste Disposal Site; (3) 04-99-01, Contaminated Surface Debris; (4) 06-19-02, Waste Disposal Site/Burn Pit; (5) 06-19-03, Waste Disposal Trenches; (6) 09-23-01, Area 9 Gravel Gertie; and (7) 09-34-01, Underground Detection Station. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives with the exception of CASs 09-23-01 and 09-34-01. Regarding these two CASs, CAS 09-23-01 is a gravel gertie where a zero-yield test was conducted with all contamination confined to below ground within the area of the structure, and CAS 09-34-01 is an underground detection station where no contaminants are present. Additional information will be obtained by conducting a corrective action investigation (CAI) before evaluating corrective action alternatives and selecting the appropriate corrective action for the other five CASs where information is insufficient. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on January 4, 2006, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and Bechtel Nevada. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 139.

Site characterization and validation - Borehole radar investigations stage 3

International Nuclear Information System (INIS)

Sandberg, E.; Olsson, O.; Falk, L.

1989-11-01

The borehole radar investigation program Stage 3 of the SCV-site has comprised single hole reflection measurements with centre frequencies of 22 and 60 MHz. Single hole reflection measurement with both omni-directional and directional antennas have been performed in the boreholes C1, C2, C3 and the D-holes. Crosshole tomographic measurements as well as cross- hole reflection measurement have been made between the bore- holes C1-C2, W1-C1 and W1-C2. The range obtained in the single hole reflection measurements was approximately 100 m for the lower frequency and about 60-70 m for the centre frequency 60 MHz. In the crosshole measurements transmitter-receiver separations from 20 to 120 m have been used. The Stage 3 radar investigations have essentially confirmed the three dimensional description of the structures at the SCV-site. The conceptual model of the site which was produced based on the Stage 1 data included three major zones, two minor zones and a circular feature. The major features are considered to be the most significant at the site and are all observed in the Stage 3 boreholes close to their predicted locations. The circular feature has also been found in two of the additional tomograms at the predicted location. The results indicate that the zones are not homogeneous but rather that they are highly irregular containing parts of considerably increased fracturing and parts where their contrast to the background rock is quite small. The zones appear to be approximately planar at least at the scale of the site. At a smaller scale the zones can appear quite irregular

Investigative studies for the use of an inactive asbestos mine as a disposal site for asbestos wastes.

Science.gov (United States)

Gidarakos, Evangelos; Anastasiadou, Kalliopi; Koumantakis, Emmanuil; Nikolaos, Stappas

2008-05-30

Although, according to European legislation the use of Asbestos Containing Materials is forbidden, many buildings in Greece still contain asbestos products, which must be removed at some point in the near future. Therefore, suitable disposal sites must be found within Greece, so that the unverified disposal of asbestos waste in municipal waste Landfills is brought to an end. In the present work, an innovative approach to the disposal problem of asbestos wastes in Greece has been examined, through a risk assessment analysis of the inactive asbestos mine of Northern Greece and an evaluation of its suitability as a disposal site for asbestos wastes in the future. According to the research carried out, two areas (Site 1 and Site 2) inside the mine area are suitable for the construction of a disposal site for asbestos wastes. The geological investigations showed that in Site 1 and Site 2 ultrabasic rocks of ophiolite complex were prevalent, which have been intensely serpentinized and converted into the fibrous shape of serpentine (asbestos). Concentrations of hazardous substances such as heavy metals in the soil of Site 1 and Site 2 oscillate at low levels, with the exception of the concentrations of nickel and chrome which are high. The investigative work also included the collection of meteorological data and the monitoring of the water level of the artificial lake, which has developed inside the open mine. The main aim is to safely dispose asbestos wastes inside the mine, to minimize any pollution of the wider vicinity of the mine, as well as to engage in restoration activities.

Active serine involved in the stabilization of the active site loop in the Humicola lanuginosa lipase

DEFF Research Database (Denmark)

Peters, Günther H.j.; Svendsen, A.; Langberg, H.

1998-01-01

We have investigated the binding properties of and dynamics in Humicola lanuginosa lipase (HII) and the inactive mutant S146A (active Ser146 substituted with Ala) using fluorescence spectroscopy and molecular dynamics simulations, respectively. Hll and S146A show significantly different binding......, whereas only small changes are observed for I-Ill suggesting that the active site Lid in the latter opens more easily and hence more lipase molecules are bound to the liposomes. These observations are in agreement with molecular dynamics simulations and subsequent essential dynamics analyses. The results...... to substantial conformational alterations in the H. lanuginosa Lipase and different binding affinities....

Corrective Action Investigation Plan for Corrective Action Unit 145: Wells and Storage Holes, Nevada Test Site, Nevada, Rev. No.: 0

Energy Technology Data Exchange (ETDEWEB)

David A. Strand

2004-09-01

This Corrective Action Investigation Plan (CAIP) contains project-specific information for conducting site investigation activities at Corrective Action Unit (CAU) 145: Wells and Storage Holes. Information presented in this CAIP includes facility descriptions, environmental sample collection objectives, and criteria for the selection and evaluation of environmental samples. Corrective Action Unit 145 is located in Area 3 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 145 is comprised of the six Corrective Action Sites (CASs) listed below: (1) 03-20-01, Core Storage Holes; (2) 03-20-02, Decon Pad and Sump; (3) 03-20-04, Injection Wells; (4) 03-20-08, Injection Well; (5) 03-25-01, Oil Spills; and (6) 03-99-13, Drain and Injection Well. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation (CAI) prior to evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. One conceptual site model with three release scenario components was developed for the six CASs to address all releases associated with the site. The sites will be investigated based on data quality objectives (DQOs) developed on June 24, 2004, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and Bechtel Nevada. The DQOs process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 145.

Promoter proximal polyadenylation sites reduce transcription activity

DEFF Research Database (Denmark)

Andersen, Pia Kjølhede; Lykke-Andersen, Søren; Jensen, Torben Heick

2012-01-01

Gene expression relies on the functional communication between mRNA processing and transcription. We previously described the negative impact of a point-mutated splice donor (SD) site on transcription. Here we demonstrate that this mutation activates an upstream cryptic polyadenylation (CpA) site......, which in turn causes reduced transcription. Functional depletion of U1 snRNP in the context of the wild-type SD triggers the same CpA event accompanied by decreased RNA levels. Thus, in accordance with recent findings, U1 snRNP can shield premature pA sites. The negative impact of unshielded pA sites...... on transcription requires promoter proximity, as demonstrated using artificial constructs and supported by a genome-wide data set. Importantly, transcription down-regulation can be recapitulated in a gene context devoid of splice sites by placing a functional bona fide pA site/transcription terminator within ∼500...

Preliminary safety evaluation for the Laxemar subarea. Based on data and site descriptions after the initial site investigation stage

Energy Technology Data Exchange (ETDEWEB)

Andersson, Johan [JA Streamflow AB, Aelvsjoe (Sweden)

2006-03-15

The main objectives of this Preliminary Safety Evaluation (PSE) of the Laxemar subarea have been to determine, with limited efforts, whether the feasibility study's judgement of the suitability of the candidate area with respect to long-term safety holds up in the light of the actual site investigation data; to provide feedback to continued site investigations and site-specific repository design and to identify site-specific scenarios and geoscientific issues for further analyses. The PSE focuses on comparing the attained knowledge of the sites with the suitability criteria as set out by SKB in 2000. These criteria both concern properties of the site judged to be necessary for safety and engineering (requirements) and properties judged to be beneficial (preferences). The findings are then evaluated in order to provide feedback to continued investigations and design work. The PSE does not aim at comparing sites and does not assess compliance with safety and radiation protection criteria. The latter is eventually done in coming Safety Assessments. This preliminary safety evaluation shows that, according to existing data, the Laxemar subarea meets all safety requirements. The evaluation also shows that the Laxemar subarea meets most of the safety preferences, but for some aspects of the site description further reduction of the uncertainties would enhance the safety case. Despite the stated concerns, there is no reason, from a safety point of view, not to continue the Site Investigations at the Laxemar subarea. There are uncertainties to resolve and the safety would eventually need to be verified through a proper safety assessment. Only some of the uncertainties noted in the Site Descriptive Model have safety implications and need further resolution for this reason. Furthermore, uncertainties may need resolving for other reasons, such as giving an adequate assurance of site understanding or assisting in optimising design. Notably, there are questions about the

Preliminary safety evaluation for the Laxemar subarea. Based on data and site descriptions after the initial site investigation stage

International Nuclear Information System (INIS)

Andersson, Johan

2006-03-01

The main objectives of this Preliminary Safety Evaluation (PSE) of the Laxemar subarea have been to determine, with limited efforts, whether the feasibility study's judgement of the suitability of the candidate area with respect to long-term safety holds up in the light of the actual site investigation data; to provide feedback to continued site investigations and site-specific repository design and to identify site-specific scenarios and geoscientific issues for further analyses. The PSE focuses on comparing the attained knowledge of the sites with the suitability criteria as set out by SKB in 2000. These criteria both concern properties of the site judged to be necessary for safety and engineering (requirements) and properties judged to be beneficial (preferences). The findings are then evaluated in order to provide feedback to continued investigations and design work. The PSE does not aim at comparing sites and does not assess compliance with safety and radiation protection criteria. The latter is eventually done in coming Safety Assessments. This preliminary safety evaluation shows that, according to existing data, the Laxemar subarea meets all safety requirements. The evaluation also shows that the Laxemar subarea meets most of the safety preferences, but for some aspects of the site description further reduction of the uncertainties would enhance the safety case. Despite the stated concerns, there is no reason, from a safety point of view, not to continue the Site Investigations at the Laxemar subarea. There are uncertainties to resolve and the safety would eventually need to be verified through a proper safety assessment. Only some of the uncertainties noted in the Site Descriptive Model have safety implications and need further resolution for this reason. Furthermore, uncertainties may need resolving for other reasons, such as giving an adequate assurance of site understanding or assisting in optimising design. Notably, there are questions about the

Double tracks test site characterization report

International Nuclear Information System (INIS)

1996-05-01

This report presents the results of site characterization activities performed at the Double Tracks Test Site, located on Range 71 North, of the Nellis Air Force Range (NAFR) in southern Nevada. Site characterization activities included reviewing historical data from the Double Tracks experiment, previous site investigation efforts, and recent site characterization data. The most recent site characterization activities were conducted in support of an interim corrective action to remediate the Double Tracks Test Site to an acceptable risk to human health and the environment. Site characterization was performed using a phased approach. First, previously collected data and historical records sere compiled and reviewed. Generalized scopes of work were then prepared to fill known data gaps. Field activities were conducted and the collected data were then reviewed to determine whether data gaps were filled and whether other areas needed to be investigated. Additional field efforts were then conducted, as required, to adequately characterize the site. Characterization of the Double Tracks Test Site was conducted in accordance with the US Department of Energy's (DOE) Streamlined Approach for Environmental Restoration (SAFER)

Description of the Northwest hazardous waste site data base and preliminary analysis of site characteristics

International Nuclear Information System (INIS)

Woodruff, D.L.; Hartz, K.E.; Triplett, M.B.

1988-08-01

The Northwest Hazardous Waste RD and D Center (the Center) conducts research, development, and demonstration (RD and D) activities for hazardous and radioactive mixed-waste technologies applicable to remediating sites in the states of Idaho, Montana, Oregon, and Washington. To properly set priorities for these RD and D activities and to target development efforts it is necessary to understand the nature of the sites requiring remediation. A data base of hazardous waste site characteristics has been constructed to facilitate this analysis. The data base used data from EPA's Region X Comprehensive Environmental Response, Compensation, and Liability Information System (CERCLIS) and from Preliminary Assessment/Site Investigation (PA/SI) forms for sites in Montana. The Center's data base focuses on two sets of sites--those on the National Priorities List (NPL) and other sites that are denoted as ''active'' CERCLIS sites. Active CERCLIS sites are those sites that are undergoing active investigation and analysis. The data base contains information for each site covering site identification and location, type of industry associated with the site, waste categories present (e.g., heavy metals, pesticides, etc.), methods of disposal (e.g., tanks, drums, land, etc.), waste forms (e.g., liquid, solid, etc.), and hazard targets (e.g., surface water, groundwater, etc.). As part of this analysis, the Northwest region was divided into three geographic subregions to identify differences in disposal site characteristics within the Northwest. 2 refs., 18 figs., 5 tabs

Corrective Action Investigation Plan for Corrective Action Unit 104: Area 7 Yucca Flat Atmospheric Test Sites, Nevada National Security Site, Nevada, Revision 0

Energy Technology Data Exchange (ETDEWEB)

Patrick Matthews

2011-08-01

CAU 104 comprises the 15 CASs listed below: (1) 07-23-03, Atmospheric Test Site T-7C; (2) 07-23-04, Atmospheric Test Site T7-1; (3) 07-23-05, Atmospheric Test Site; (4) 07-23-06, Atmospheric Test Site T7-5a; (5) 07-23-07, Atmospheric Test Site - Dog (T-S); (6) 07-23-08, Atmospheric Test Site - Baker (T-S); (7) 07-23-09, Atmospheric Test Site - Charlie (T-S); (8) 07-23-10, Atmospheric Test Site - Dixie; (9) 07-23-11, Atmospheric Test Site - Dixie; (10) 07-23-12, Atmospheric Test Site - Charlie (Bus); (11) 07-23-13, Atmospheric Test Site - Baker (Buster); (12) 07-23-14, Atmospheric Test Site - Ruth; (13) 07-23-15, Atmospheric Test Site T7-4; (14) 07-23-16, Atmospheric Test Site B7-b; (15) 07-23-17, Atmospheric Test Site - Climax These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on April 28, 2011, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 104. The releases at CAU 104 consist of surface-deposited radionuclides from 30 atmospheric nuclear tests. The presence and nature of contamination at CAU 104 will be evaluated based on information collected from a field investigation. Radiological contamination will be evaluated based on a comparison

Corrective Action Investigation Plan for Corrective Action Unit 552: Area 12 Muckpile and Ponds, Nevada Test Site, Nevada: Revision 0

Energy Technology Data Exchange (ETDEWEB)

U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office

2004-04-06

This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office's approach for collecting the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 552: Area 12 Muckpile and Ponds, Nevada Test Site (NTS), Nevada, under the Federal Facility Agreement and Consent Order. Located in Area 12 on the NTS, CAU 552 consists of two Corrective Action Sites (CASs): 12-06-04, Muckpile; 12-23-05, Ponds. Corrective Action Site 12-06-04 in Area 12 consists of the G-Tunnel muckpile, which is the result of tunneling activities. Corrective Action Site 12-23-05 consists of three dry ponds adjacent to the muckpile. The toe of the muckpile extends into one of the ponds creating an overlap of two CASs. The purpose of the investigation is to ensure that adequate data are collected to provide sufficient and reliable information to identify, evaluate, and select technic ally viable corrective actions. The results of the field investigation will support a defensible evaluation of corrective action alternatives in the corrective action decision document.

MX Siting Investigation. MX System Siting Summary Report. General Introduction. Volume I. Part I.

Science.gov (United States)

1982-01-18

LINEAR CONNECT CRN MIX SITING INVESTIGATION DEPARTMENT OF THE AIR FORCE VOUTS USING VALLEY CLUSTERING CONCEPT IN IOC, BMO/AFRCE- wIX VOUTS IN DRY LAKE...species, such as the jackrabbit, may be the center of important food webs , and a decrease in its numbers may greatly affect many other species. The

Corrective Action Investigation Plan for Corrective Action Unit 541: Small Boy Nevada National Security Site and Nevada Test and Training Range, Nevada with ROTC 1

Energy Technology Data Exchange (ETDEWEB)

Matthews, Patrick [Navarro-Intera, LLC (N-I), Las Vegas, NV (United States)

2014-09-01

Corrective Action Unit (CAU) 541 is co-located on the boundary of Area 5 of the Nevada National Security Site and Range 65C of the Nevada Test and Training Range, approximately 65 miles northwest of Las Vegas, Nevada. CAU 541 is a grouping of sites where there has been a suspected release of contamination associated with nuclear testing. This document describes the planned investigation of CAU 541, which comprises the following corrective action sites (CASs): 05-23-04, Atmospheric Tests (6) - BFa Site; 05-45-03, Atmospheric Test Site - Small Boy. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the investigation report. The sites will be investigated based on the data quality objectives (DQOs) developed on April 1, 2014, by representatives of the Nevada Division of Environmental Protection; U.S. Air Force; and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Field Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 541. The site investigation process also will be conducted in accordance with the Soils Activity Quality Assurance Plan, which establishes requirements, technical planning, and general quality practices to be applied to this activity. The potential contamination sources associated with CASs 05-23-04 and 05-45-03 are from nuclear testing activities conducted at the Atmospheric Tests (6) - BFa Site and Atmospheric Test Site - Small Boy sites. The presence and nature of

Bedrock Hydrogeology-Site investigation SFR

International Nuclear Information System (INIS)

Oehman, Johan; Bockgaard, Niclas; Follin, Sven

2012-06-01

The Swedish Nuclear Fuel and Waste Management Company (SKB) has conducted site investigations for a planned extension of the existing final repository for short-lived radioactive waste (SFR). This report presents an integrated analysis and interpretation of the historic data from the existing SFR (1980 - 1986), as well as, from the recent investigations for the planned extension of SFR (2008 - 2009). The primary objective is to establish a conceptual hydrogeological model of the bedrock for safety assessment and design analyses. Analyses and interpretations of all (old and new) hydraulic data are analysed with regard to the recently developed geological deformation zone model of the SFR model domain (Curtis et al. 2011). The methodology used by Curtis et al. (2011) has focussed on magnetic anomalies and deformation zone intercepts with ground surface greater than 300 m. In the hydrogeological modelling, however, it has been considered important to also explore the occurrence and characteristics of shallow horizontal to sub-horizontal structures (sheet joints) inside the SFR model domain. Such structures are of considerable importance for the hydrogeology in the uppermost c. 150 m of bedrock in SDM-Site Forsmark; hence the term Shallow Bedrock Aquifer was used to emphasise their hydraulic significance. In this study, the acronym SBA-structure is used for horizontal structures identified in the hydrogeological modelling. In addition to the predominantly steeply dipping geological deformation zones, eight so-called SBA-structures are modelled deterministically in the hydrogeological model. The SBA-structures are envisaged as hydraulically heterogeneous and composed of clusters of minor gently dipping to horizontal fractures rather than extensive single features. A type of structures that is partly included in the definition of the SBA-structures is the Unresolved Possible Deformations Zone (Unresolved PDZ) intercepts identified by Curtis et al. (2011). The Unresolved

Bedrock Hydrogeology - Site investigation SFR

Energy Technology Data Exchange (ETDEWEB)

Oehman, Johan [Geosigma AB, Stockholm (Sweden); Bockgaard, Niclas [Golder Assoes AB, Stockholm (Sweden); Follin, Sven [SF GeoLogic AB, Taeby (Sweden)

2012-06-15

The Swedish Nuclear Fuel and Waste Management Company (SKB) has conducted site investigations for a planned extension of the existing final repository for short-lived radioactive waste (SFR). This report presents an integrated analysis and interpretation of the historic data from the existing SFR (1980 - 1986), as well as, from the recent investigations for the planned extension of SFR (2008 - 2009). The primary objective is to establish a conceptual hydrogeological model of the bedrock for safety assessment and design analyses. Analyses and interpretations of all (old and new) hydraulic data are analysed with regard to the recently developed geological deformation zone model of the SFR model domain (Curtis et al. 2011). The methodology used by Curtis et al. (2011) has focussed on magnetic anomalies and deformation zone intercepts with ground surface greater than 300 m. In the hydrogeological modelling, however, it has been considered important to also explore the occurrence and characteristics of shallow horizontal to sub-horizontal structures (sheet joints) inside the SFR model domain. Such structures are of considerable importance for the hydrogeology in the uppermost c. 150 m of bedrock in SDM-Site Forsmark; hence the term Shallow Bedrock Aquifer was used to emphasise their hydraulic significance. In this study, the acronym SBA-structure is used for horizontal structures identified in the hydrogeological modelling. In addition to the predominantly steeply dipping geological deformation zones, eight so-called SBA-structures are modelled deterministically in the hydrogeological model. The SBA-structures are envisaged as hydraulically heterogeneous and composed of clusters of minor gently dipping to horizontal fractures rather than extensive single features. A type of structures that is partly included in the definition of the SBA-structures is the Unresolved Possible Deformations Zone (Unresolved PDZ) intercepts identified by Curtis et al. (2011). The Unresolved

Review of the ANSTO submission on the site geological investigations for the RRR at Lucas Heights

International Nuclear Information System (INIS)

2002-01-01

Preliminary Safety Analysis Report (PSAR) and ANSTO's Consolidated Seismic Report were reviewed by ARPANSA's Regulatory Branch, and by an IAEA consultant to ARPANSA. It included a recommendation by the IAEA Peer Review Team (June 2001) that an additional study be undertaken for the Lucas Heights site in accordance with IAEA Safety Guide 50-SG-S1. In particular this related to regional and local investigations to determine whether there exists a potential for surface faulting at the site area, to formulate the investigations that should be used to determine whether or not any faults in the area should be considered capable. The CEO of ARPANSA requested ANSTO to undertake such a task. The reviews that have been undertaken by experts from Geoscience Australia and the IAEA expert contracted by ARPANSA give confidence that the seismic design of the reactor structures, systems, and components important for safety should not be affected by the fault through the excavations for the foundations of the reactor building. The basis is the conclusions reached by these experts that the fault discovered is very old and not capable under either the USNRC Criteria or the IAEA Criteria. It is not now active, has not been active in the geologically recent past and is unlikely to re-activate within the foreseeable lifespan of the proposed facility. Consequently, this pre-existing fault presents no greater risk than the surrounding unfaulted material. Thus, the replacement reactor need not be designed for such displacement. The existence of this 'incapable' fault does not impact on the probabilistic seismic hazard assessment from which the seismic design basis for the reactor was derived. Therefore, the existing design response spectrum accepted in the Regulatory Assessment Report as the basis for seismic design of structures, systems and components, and approved in the Construction Licence, has not changed and remains adequately conservative with regard to vibratory motion at the site

Active Sites Environmental Monitoring Program: Mid-FY 1991 report

Energy Technology Data Exchange (ETDEWEB)

Ashwood, T.L.; Wickliff, D.S.; Morrissey, C.M.

1991-10-01

This report summarizes the activities of the Active Sites Environmental Monitoring Program (ASEMP) from October 1990 through March 1991. The ASEMP was established in 1989 by Solid Waste Operations and the Environmental Sciences Division to provide early detection and performance monitoring at active low-level radioactive waste (LLW) disposal sites in Solid Waste Storage Area (SWSA) 6 and transuranic (TRU) waste storage sites in SWSA 5 as required by chapters II and III of US Department of Energy Order 5820.2A. Monitoring results continue to demonstrate the no LLW is being leached from the storage vaults on the tumulus pads. Loading of vaults on Tumulus II began during this reporting period and 115 vaults had been loaded by the end of March 1991.

Molecular dynamics explorations of active site structure in designed and evolved enzymes.

Science.gov (United States)

Osuna, Sílvia; Jiménez-Osés, Gonzalo; Noey, Elizabeth L; Houk, K N

2015-04-21

This Account describes the use of molecular dynamics (MD) simulations to reveal how mutations alter the structure and organization of enzyme active sites. As proposed by Pauling about 70 years ago and elaborated by many others since then, biocatalysis is efficient when functional groups in the active site of an enzyme are in optimal positions for transition state stabilization. Changes in mechanism and covalent interactions are often critical parts of enzyme catalysis. We describe our explorations of the dynamical preorganization of active sites using MD, studying the fluctuations between active and inactive conformations normally concealed to static crystallography. MD shows how the various arrangements of active site residues influence the free energy of the transition state and relates the populations of the catalytic conformational ensemble to the enzyme activity. This Account is organized around three case studies from our laboratory. We first describe the importance of dynamics in evaluating a series of computationally designed and experimentally evolved enzymes for the Kemp elimination, a popular subject in the enzyme design field. We find that the dynamics of the active site is influenced not only by the original sequence design and subsequent mutations but also by the nature of the ligand present in the active site. In the second example, we show how microsecond MD has been used to uncover the role of remote mutations in the active site dynamics and catalysis of a transesterase, LovD. This enzyme was evolved by Tang at UCLA and Codexis, Inc., and is a useful commercial catalyst for the production of the drug simvastatin. X-ray analysis of inactive and active mutants did not reveal differences in the active sites, but relatively long time scale MD in solution showed that the active site of the wild-type enzyme preorganizes only upon binding of the acyl carrier protein (ACP) that delivers the natural acyl group to the active site. In the absence of bound ACP

Active hydrothermal and non-active massive sulfide mound investigation using a new multiparameter chemical sensor

Science.gov (United States)

Han, C.; Wu, G.; Qin, H.; Wang, Z.

2012-12-01

Investigation of active hydrothermal mound as well as non-active massive sulfide mound are studied recently. However, there is still lack of in-situ detection method for the non-active massive sulfide mound. Even though Transient ElectroMagnetic (TEM) and Electric Self-potential (SP) methods are good, they both are labour, time and money cost work. We proposed a new multiparameter chemical sensor method to study the seafloor active hydrothermal mound as well as non-active massive sulfide mound. This sensor integrates Eh, S2- ions concentration and pH electrochemical electrodes together, and could found chemical change caused by the active hydrothermal vent, even weak chemical abnormalities by non-active massive sulfide hydrothermal mound which MARP and CTD sometimes cannot detect. In 2012, the 1st Leg of the Chinese 26th cruise, the multiparameter chemical sensor was carried out with the deepsea camera system over the Carlsberg Ridge in Indian Ocean by R/V DAYANGYIHAO. It was shown small Eh and S2- ions concentration abnormal around a site at Northwest Indian ridge. This site was also evidenced by the TV grab. In the 2nd Leg of the same cruise in June, this chemical sensor was carried out with TEM and SP survey system. The chemical abnormalities are matched very well with both TEM and SP survey results. The results show that the multiparameter chemical sensor method not only can detect active hydrothermal mound, but also can find the non-active massive sulfide hydrothermal mound.

Active Sites Environmental Monitoring Program: FY 1990 annual report

International Nuclear Information System (INIS)

Wickliff, D.S.; Morrissey, C.M.; Ashwood, T.L.

1991-10-01

Chapter 3 of US Department of Energy (DOE) Order 5820.2A (DOE 1988) sets forth requirements for environmental monitoring of active low-level waste (LLW) disposal sites. Active sites are defined as those LLW facilities that were in use on or after the date of the order (September 1988). The transuranic (TRU) waste storage areas in Solid Waste Storage Area (SWSA) 5 North are covered by Chap. 2 of the order. In both chapters, monitoring is required to provide for early warning of leaks before those leaks pose a threat to human health or the environment. Chapter 3 also requires that monitoring be conducted to evaluate the short- and long-term performance of LLW disposal facilities. In accordance with this order, the Solid Waste Operations Department at Oak Ridge National Laboratory (ORNL) has established an Active Sites Environmental Monitoring Program (ASEMP) that is implemented by staff of the Environmental Sciences Division (ESD) at ORNL. This report summarizes data from ASEMP monitoring activities for the final 6 months of FY 1990. A brief summary of the monitoring methodology for each site is presented also

Corrective Action Investigation Plan for Corrective Action Unit 487: Thunderwell Site, Tonopah Test Range, Nevada (Rev. No.: 0, January 2001); TOPICAL

International Nuclear Information System (INIS)

2001-01-01

This Corrective Action Investigation Plan contains the U.S. Department of Energy, Nevada Operations Office's (DOE/NV's) approach to collect the data necessary to evaluate corrective action alternatives (CAAs) appropriate for the closure of Corrective Action Unit (CAU) 487, Thunderwell Site, Tonopah Test Range (TTR), Nevada, under the Federal Facility Agreement and Consent Order. Corrective Action Unit 487 consists of a single Corrective Action Site (CAS), RG 26-001-RGRV, Thunderwell Site. The site is located in the northwest portion of the TTR, Nevada, approximately five miles northwest of the Area 3 Control Point and closest to the Cactus Flats broad basin. Historically, Sandia National Laboratories in New Mexico used CAU 487 in the early to mid-1960s for a series of high explosive tests detonated at the bottom of large cylindrical steel tubes. Historical photographs indicate that debris from these tests and subsequent operations may have been scattered and buried throughout the site. A March 2000 walk-over survey and a July 2000 geophysical survey indicated evidence of buried and surface debris in dirt mounds and areas throughout the site; however, a radiological drive-over survey also performed in July 2000 indicated that no radiological hazards were identified at this site. Based on site history, the scope of this plan is to resolve the problem statement identified during the Data Quality Objectives process that detonation activities at this CAU site may have resulted in the release of contaminants of concern into the surface/subsurface soil including total volatile and total semivolatile organic compounds, total Resource Conservation and Recovery Act metals, radionuclides, total petroleum hydrocarbons, and high explosives. Therefore, the scope of corrective action field investigation will involve excavation, drilling, and extensive soil sampling and analysis activities to determine the extent (if any) of both the lateral and vertical contamination and whether

Correlated compositional and mineralogical investigations at the Chang′e-3 landing site

Science.gov (United States)

Ling, Zongcheng; Jolliff, Bradley L.; Wang, Alian; Li, Chunlai; Liu, Jianzhong; Zhang, Jiang; Li, Bo; Sun, Lingzhi; Chen, Jian; Xiao, Long; Liu, Jianjun; Ren, Xin; Peng, Wenxi; Wang, Huanyu; Cui, Xingzhu; He, Zhiping; Wang, Jianyu

2015-01-01

The chemical compositions of relatively young mare lava flows have implications for the late volcanism on the Moon. Here we report the composition of soil along the rim of a 450-m diameter fresh crater at the Chang′e-3 (CE-3) landing site, investigated by the Yutu rover with in situ APXS (Active Particle-induced X-ray Spectrometer) and VNIS (Visible and Near-infrared Imaging Spectrometer) measurements. Results indicate that this region's composition differs from other mare sample-return sites and is a new type of mare basalt not previously sampled, but consistent with remote sensing. The CE-3 regolith derived from olivine-normative basaltic rocks with high FeO/(FeO+MgO). Deconvolution of the VNIS data indicates abundant high-Ca ferropyroxene (augite and pigeonite) plus Fe-rich olivine. We infer from the regolith composition that the basaltic source rocks formed during late-stage magma-ocean differentiation when dense ferropyroxene-ilmenite cumulates sank and mixed with deeper, relatively ferroan olivine and orthopyroxene in a hybridized mantle source. PMID:26694712

Correlated compositional and mineralogical investigations at the Chang'e-3 landing site.

Science.gov (United States)

Ling, Zongcheng; Jolliff, Bradley L; Wang, Alian; Li, Chunlai; Liu, Jianzhong; Zhang, Jiang; Li, Bo; Sun, Lingzhi; Chen, Jian; Xiao, Long; Liu, Jianjun; Ren, Xin; Peng, Wenxi; Wang, Huanyu; Cui, Xingzhu; He, Zhiping; Wang, Jianyu

2015-12-22

The chemical compositions of relatively young mare lava flows have implications for the late volcanism on the Moon. Here we report the composition of soil along the rim of a 450-m diameter fresh crater at the Chang'e-3 (CE-3) landing site, investigated by the Yutu rover with in situ APXS (Active Particle-induced X-ray Spectrometer) and VNIS (Visible and Near-infrared Imaging Spectrometer) measurements. Results indicate that this region's composition differs from other mare sample-return sites and is a new type of mare basalt not previously sampled, but consistent with remote sensing. The CE-3 regolith derived from olivine-normative basaltic rocks with high FeO/(FeO+MgO). Deconvolution of the VNIS data indicates abundant high-Ca ferropyroxene (augite and pigeonite) plus Fe-rich olivine. We infer from the regolith composition that the basaltic source rocks formed during late-stage magma-ocean differentiation when dense ferropyroxene-ilmenite cumulates sank and mixed with deeper, relatively ferroan olivine and orthopyroxene in a hybridized mantle source.

Tritium activities in selected wells on the Nevada Test Site

International Nuclear Information System (INIS)

Lyles, B.F.

1993-05-01

Literature and data were reviewed related to radionuclides in groundwater on and near the Nevada Test Site. No elevated tritium activities have been reported outside of the major testing regions of the Nevada Test Site. Three wells were identified as having water with above-background (>50 pCi/l) tritium activities: UE-15d Water Well; USGS Water Well A; and USGS Test Well B Ex. Although none of these wells have tritium activities greater than the Nevada State Drinking Water standard (20,000 pCi/l), their time-series tritium trends may be indicative to potential on-site radionuclide migration

Investigation of surface and underground waters about the Blayais nuclear site - 2010

International Nuclear Information System (INIS)

Migeon, A.; Bernollin, A.; Dunand, E.; Barbey, P.; Boilley, D.; Josset, M.

2011-01-01

This investigation aims at proposing a first assessment of the impact of releases on surface and underground waters around the Blayais nuclear power station, i.e. the assessment of the (mainly radiological) quality of waters. The report identifies the various pollution sources: old sources (like atmospheric nuclear tests, nuclear accidents), incidents in the Blayais station, and potential sources for the present contamination. Different radionuclides are searched like tritium, carbon 14, gamma radioactivity (from different elements), some beta emitters, radon as well as some chemicals related to the station activity (hydrazine, boric acid, EDTA, lithium, morpholine). Sampling sites are presented (estuary, canals, reservoirs). Radiological and chemical analysis are reported and commented. Significant presence of Tritium and Nickel-63 are noticed

Development of METAL-ACTIVE SITE and ZINCCLUSTER tool to predict active site pockets.

Science.gov (United States)

Ajitha, M; Sundar, K; Arul Mugilan, S; Arumugam, S

2018-03-01

The advent of whole genome sequencing leads to increasing number of proteins with known amino acid sequences. Despite many efforts, the number of proteins with resolved three dimensional structures is still low. One of the challenging tasks the structural biologists face is the prediction of the interaction of metal ion with any protein for which the structure is unknown. Based on the information available in Protein Data Bank, a site (METALACTIVE INTERACTION) has been generated which displays information for significant high preferential and low-preferential combination of endogenous ligands for 49 metal ions. User can also gain information about the residues present in the first and second coordination sphere as it plays a major role in maintaining the structure and function of metalloproteins in biological system. In this paper, a novel computational tool (ZINCCLUSTER) is developed, which can predict the zinc metal binding sites of proteins even if only the primary sequence is known. The purpose of this tool is to predict the active site cluster of an uncharacterized protein based on its primary sequence or a 3D structure. The tool can predict amino acids interacting with a metal or vice versa. This tool is based on the occurrence of significant triplets and it is tested to have higher prediction accuracy when compared to that of other available techniques. © 2017 Wiley Periodicals, Inc.

Site-specific investigations of aquifer thermal energy storage for space and process cooling

International Nuclear Information System (INIS)

Brown, D.R.

1991-01-01

This paper reports on the Pacific Northwest Laboratory (PNL) that has completed three preliminary site-specific feasibility studies that investigated aquifer thermal energy storage (ATES) for reducing space and process cooling costs. Chilled water stored in an ATES system could be used to meet all or part of the process and/or space cooling loads at the three facilities investigated. Seasonal or diurnal chill ATES systems could be significantly less expensive than a conventional electrically-driven, load-following chiller system at one of the three sites, depending on the cooling water loop return temperature and presumed future electricity escalation rate. For the other two sites investigated, a chill ATES system would be economically competitive with conventional chillers if onsite aquifer characteristics were improved. Well flow rates at one of the sites were adequate, but the expected thermal recovery efficiency was too low. The reverse of this situation was found at the other site, where the thermal recovery efficiency was expected to be adequate, but well flow rates were too low

Visualization of the Differential Transition State Stabilization within the Active Site Environment

Directory of Open Access Journals (Sweden)

Jerzy Leszczynski

2004-05-01

Full Text Available Abstract: Increasing interest in the enzymatic reaction mechanisms and in the nature of catalytic effects in enzymes causes the need of appropriate visualization methods. A new interactive method to investigate catalytic effects using differential transition state stabilization approach (DTSS [1, 2] is presented. The catalytic properties of the active site of cytidine deaminase (E.C. 3.5.4.5 is visualized in the form of differential electrostatic properties. The visualization was implemented using scripting interface of VMD [3]. Cumulative Atomic Multipole Moments (CAMM [4,5,6] were utilized for efficient yet accurate evaluation of the electrostatic properties. The implementation is efficient enough for interactive presentation of catalytic effects in the active site of the enzyme due to transition state or substrate movement. This system of visualization of DTTS approach can be potentially used to validate hypotheses regarding the catalytic mechanism or to study binding properties of transition state analogues.

Animal investigation program 1980 annual report: Nevada Test Site and vicinity

International Nuclear Information System (INIS)

Smith, D.D.; Bernhardt, D.E.; Giles, K.R.

1982-07-01

This report summarizes the data collected through the Animal Investigation Program during 1980. A major goal of the Program is to assess the radionuclide burden in the tissues of wild and domesticated animals around the Nevada Test Site and to detect pathological effects resulting from the burdens. Other than naturally occurring potassium-40, gamma emitting radionuclides were detected infrequently. Strontium-90 and plutonium concentrations in tissues from deer, cattle, and desert bighorn sheep were similar to those found in samples collected during recent years. Lesions found in necropsied animals were similar to those found in animals from other areas of the U.S. and would not be attributable to ionizing radiation exposure. The report also describes other activities of the program, including the deer migration study and census

Zymogen Activation and Subcellular Activity of Subtilisin Kexin Isozyme 1/Site 1 Protease*

Science.gov (United States)

da Palma, Joel Ramos; Burri, Dominique Julien; Oppliger, Joël; Salamina, Marco; Cendron, Laura; de Laureto, Patrizia Polverino; Seidah, Nabil Georges; Kunz, Stefan; Pasquato, Antonella

2014-01-01

The proprotein convertase subtilisin kexin isozyme 1 (SKI-1)/site 1 protease (S1P) plays crucial roles in cellular homeostatic functions and is hijacked by pathogenic viruses for the processing of their envelope glycoproteins. Zymogen activation of SKI-1/S1P involves sequential autocatalytic processing of its N-terminal prodomain at sites B′/B followed by the herein newly identified C′/C sites. We found that SKI-1/S1P autoprocessing results in intermediates whose catalytic domain remains associated with prodomain fragments of different lengths. In contrast to other zymogen proprotein convertases, all incompletely matured intermediates of SKI-1/S1P showed full catalytic activity toward cellular substrates, whereas optimal cleavage of viral glycoproteins depended on B′/B processing. Incompletely matured forms of SKI-1/S1P further process cellular and viral substrates in distinct subcellular compartments. Using a cell-based sensor for SKI-1/S1P activity, we found that 9 amino acid residues at the cleavage site (P1–P8) and P1′ are necessary and sufficient to define the subcellular location of processing and to determine to what extent processing of a substrate depends on SKI-1/S1P maturation. In sum, our study reveals novel and unexpected features of SKI-1/S1P zymogen activation and subcellular specificity of activity toward cellular and pathogen-derived substrates. PMID:25378398

Active sites environmental monitoring program FY 1997 annual report

International Nuclear Information System (INIS)

Morrissey, C.M.; Marshall, D.S.; Cunningham, G.R.

1998-03-01

This report summarizes the activities conducted by the Active Sites Environmental Monitoring Program (ASEMP) from October 1996 through September 1997. The purpose of the program is to provide early detection and performance monitoring at active low-level waste (LLW) disposal sites in Solid Waste Storage Area (SWSA) 6 and transuranic (TRU) waste storage sites in SWSA 5 North. This report continues a series of annual and semiannual reports that present the results of ASEMP monitoring activities. This report details monitoring results for fiscal year (FY) 1997 from SWSA 6, including the Interim Waste Management Facility (IWMF) and the Hillcut Disposal Test Facility (HDTF), and (2) TRU-waste storage areas in SWSA 5 N. This report presents a summary of the methodology used to gather data for each major area along with the FY 1997 results. Figures referenced in the text are found in Appendix A and data tables are presented in Appendix B

Maxey Flats low-level waste disposal site closure activities

International Nuclear Information System (INIS)

Haight, C.P.; Mills, D.; Razor, J.E.

1987-01-01

The Maxey Flats Radioactive Waste Disposal Facility in Fleming County, Kentucky is in the process of being closed. The facility opened for commercial business in the spring of 1963 and received approximately 4.75 million cubic feet of radioactive waste by the time it was closed in December of 1977. During fourteen years of operation approximately 2.5 million curies of by-product material, 240,000 kilograms of source material, and 430 kilograms of special nuclear material were disposed. The Commonwealth purchased the lease hold estate and rights in May 1978 from the operating company. This action was taken to stabilize the facility and prepare it for closure consisting of passive care and monitoring. To prepare the site for closure, a number of remedial activities had to be performed. The remediation activities implemented have included erosion control, surface drainage modifications, installation of a temporary plastic surface cover, leachate removal, analysis, treatment and evaporation, US DOE funded evaporator concentrates solidification project and their on-site disposal in an improved disposal trench with enhanced cover for use in a humid environment situated in a fractured geology, performance evaluation of a grout injection demonstration, USGS subsurface geologic investigation, development of conceptual closure designs, and finally being added to the US EPA National Priority List for remediation and closure under Superfund. 13 references, 3 figures

Unraveling the Nature of Sites Active toward Hydrogen Peroxide Reduction in Fe?N?C Catalysts

OpenAIRE

Choi, Chang Hyuck; Choi, Won Seok; Kasian, Olga; Mechler, Anna K.; Sougrati, Moulay Tahar; Br?ller, Sebastian; Strickland, Kara; Jia, Qingying; Mukerjee, Sanjeev; Mayrhofer, Karl J. J.; Jaouen, Fr?d?ric

2017-01-01

Abstract Fe?N?C catalysts with high O2 reduction performance are crucial for displacing Pt in low?temperature fuel cells. However, insufficient understanding of which reaction steps are catalyzed by what sites limits their progress. The nature of sites were investigated that are active toward H2O2 reduction, a key intermediate during indirect O2 reduction and a source of deactivation in fuel cells. Catalysts comprising different relative contents of FeN x C y moieties and Fe particles encapsu...

On the activation of Pt/Al2O3 catalysts in HC-SCR by sintering. Determination of redox-active sites using Multitrack

International Nuclear Information System (INIS)

Vaccaro, A.R.; Mul, G.; Moulijn, J.A.; Perez-Ramirez, J.

2003-01-01

A highly dispersed Pt/Al 2 O 3 catalyst was used for the selective catalytic reduction of NO x using propene (HC-SCR). Contact with the reaction gas mixture led to a significant activation of the catalyst at temperatures above 523K. According to CO chemisorption data and HRTEM analysis, Pt particles on the activated catalyst had sintered. The redox behavior of the fresh and sintered catalysts was investigated using Multitrack, a TAP-like pulse reactor. If Pt particles on the catalyst are highly dispersed (average size below =2nm), only a small part (=10%) of the total number of Pt surface sites as determined by CO chemisorption (Pt surf ) participates in H 2 /O 2 redox cycles (Pt surf,redox ) in Multitrack conditions. For a sintered catalyst, with an average particle size of 2.7nm, the number of Pt surf and Pt surf,redox sites are in good agreement. Similar results were obtained for both catalysts using NO as the oxidant. The low number of Pt surf,redox sites on highly dispersed Pt/Al 2 O 3 is explained by the presence of a kinetically more stable-probably ionic-form of Pt-O bonds on all surface sites of the smaller Pt particles, including corner, edge and terrace sites. When the average particle size shifts to =2.7nm, the kinetic stability of all Pt-O bonds is collectively decreased, enabling the participation of all Pt surface sites in the redox cycles. A linear correlation between the NO x conversion in HC-SCR, and the amount of Pt surf,redox was found. This suggests that redox-active Pt sites are necessary for catalytic activity. In addition, the correlation could be significantly improved by assuming that Pt surf,terrace sites of the particles larger than 2.7nm are mainly responsible for HC-SCR activity in steady state conditions. Implications of these results for the pathway of HC-SCR over Pt catalysts are discussed

Corrective Action Investigation Plan for Corrective Action Unit 214: Bunkers and Storage Areas Nevada Test Site, Nevada

International Nuclear Information System (INIS)

2003-01-01

This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 214 under the Federal Facility Agreement and Consent Order. Located in Areas 5, 11, and 25 of the Nevada Test Site, CAU 214 consists of nine Corrective Action Sites (CASs): 05-99-01, Fallout Shelters; 11-22-03, Drum; 25-99-12, Fly Ash Storage; 25-23-01, Contaminated Materials; 25-23-19, Radioactive Material Storage; 25-99-18, Storage Area; 25-34-03, Motor Dr/Gr Assembly (Bunker); 25-34-04, Motor Dr/Gr Assembly (Bunker); and 25-34-05, Motor Dr/Gr Assembly (Bunker). These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). The suspected contaminants and critical analyte s for CAU 214 include oil (total petroleum hydrocarbons-diesel-range organics [TPH-DRO], polychlorinated biphenyls [PCBs]), pesticides (chlordane, heptachlor, 4,4-DDT), barium, cadmium, chronium, lubricants (TPH-DRO, TPH-gasoline-range organics [GRO]), and fly ash (arsenic). The land-use zones where CAU 214 CASs are located dictate that future land uses will be limited to nonresidential (i.e., industrial) activities. The results of this field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the corrective action decision document

Corrective Action Investigation Plan for Corrective Action Unit 568: Area 3 Plutonium Dispersion Sites Nevada National Security Site, Nevada, Revision 0

Energy Technology Data Exchange (ETDEWEB)

Matthews, Patrick

2014-01-01

CAU 568 is a grouping of sites where there has been a suspected release of contamination associated with nuclear testing. This document describes the planned investigation of CAU 568, which comprises the following corrective action sites (CASs): • 03-23-17, S-3I Contamination Area • 03-23-19, T-3U Contamination Area • 03-23-20, Otero Contamination Area • 03-23-22, Platypus Contamination Area • 03-23-23, San Juan Contamination Area • 03-23-26, Shrew/Wolverine Contamination Area These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the investigation report.

Active site architecture of a sugar N-oxygenase.

Science.gov (United States)

Thoden, James B; Branch, Megan C; Zimmer, Alex L; Bruender, Nathan A; Holden, Hazel M

2013-05-14

KijD3 is a flavin-dependent N-oxygenase implicated in the formation of the nitro-containing sugar d-kijanose, found attached to the antibiotic kijanimicin. For this investigation, the structure of KijD3 in complex with FMN and its dTDP-sugar substrate was solved to 2.1 Å resolution. In contrast to the apoenzyme structure, the C-terminus of the protein becomes ordered and projects into the active site cleft [Bruender, N. A., Thoden, J. B., and Holden, H. M. (2010) Biochemistry 49, 3517-3524]. The amino group of the dTDP-aminosugar that is oxidized is located 4.9 Å from C4a of the flavin ring. The model provides a molecular basis for understanding the manner in which KijD3 catalyzes its unusual chemical transformation.

Integrated account of method, site selection and programme prior to the site investigation phase[Planning for a Swedish repository for spent nuclear fuels

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-12-01

In order to dispose of the spent nuclear fuel in a safe manner, SKB plans to site a deep repository and an encapsulation plant with associated canister fabrication and transportation system. After an integrated evaluation of feasibility studies and other material, SKB will proceed with investigations of the rock and studies regarding establishment of the deep disposal system in the municipality of Oskarshamn or in Northern Uppland. The plans also include further study of the prospects for a deep repository in the municipality of Nykoeping. In the municipality of Oskarshamn, SKB plans further studies of a siting of the deep repository at Simpevarp. There SKB wants to initiate site investigations with test drilling. For the encapsulation plant, SKB wants to continue studying a siting at CLAB. In Northern Uppland, SKB plans to study two siting alternatives for the deep repository. One is Forsmark in the municipality of Oesthammar, where SKB wants to initiate a site investigation with test drilling. The other is Tierp north of Skutskaer, where SKB intends to start test drilling in an area north of Tierp. First, however, a suitable drilling area with possible transport solutions needs to be defined. This alternative requires the participation of the municipalities of both Tierp and Aelvkarleby. A siting of the encapsulation plant in Northern Uppland will also be studied. For the municipality of Nykoeping, SKB plans to conduct a new safety assessment for the Fjaellveden area, based on data from previous investigations as well as additional studies of how a deep repository could be arranged. SKB will thereby gather data from yet another geographic and geological region beyond those that are prioritized. No test drilling is planned in Nykoeping. The goal of the site investigation phase is to obtain all permits needed to build the planned facilities. It will take an estimated 7 - 8 years to assemble the requisite supporting material, carry out consultations, compile siting

Investigation of the site selection examples adopted local participation. The site selection processes in Belgium, UK and Switzerland

International Nuclear Information System (INIS)

Kageyama, Hitoshi; Suzuki, Shinji; Hirose, Ikuro; Yoshioka, Tatsuji

2014-06-01

In late years, local participation policies are being adopted in foreign countries at site selection for the disposal of the radioactive waste. We performed documents investigation about the examples of the site selection processes of Belgium, the U.K., and Switzerland to establish the site selection policy in Japan. In Belgium, after the failure of the site selection for the disposal of short-lived low and intermediate level radioactive waste (LILW) in an early stage, the idea of the local partnership (LP) was developed and three independent LPs were established between the implementing body and each municipality. About 7 years later, one site was decided as the disposal site in the cabinet meeting of the federal government. In the U.K., after the failure of the site selection for the rock characterization facility, the government policy was changed and the consultation process comprised of six phases was started. Though the process had been carried out for over 4 years since one combined partnership was established between the implementing body and the municipalities involved, they had to withdraw from the consulting process because a county council had not accepted that the process would step forward to the 4th phase. In Switzerland, the implementing body selected one site for LILW disposal at an early stage, but the project was denied by the referendum in the Canton having jurisdiction over the site area. After that the Federal Parliament established new Nuclear Energy Act and Nuclear Energy Ordinance precluding the veto of Canton. Now the site selection project is being carried out according to the process comprised of three phases with local participation policy. Reviewing the merits and demerits of each example through this investigation, we confirmed if we are to adopt local participation policy in our country in future, further prudent study would be necessary, considering current and future social conditions in Japan. (author)

Forsmark site investigation. Investigation of marine and lacustrine sediment in lakes. Field data 2003

Energy Technology Data Exchange (ETDEWEB)

Hedenstroem, Anna [SGU, Uppsala (Sweden)

2003-04-01

The aim of this activity is to describe the aerial and stratigraphical distribution of marine and lacustrine sediment i.e. sediment overlaying the glacial till and/or bedrock surface, in lakes in the Forsmark area. The investigation is carried out within areas where mapping of unconsolidated Quaternary deposits is presently carried out. Since small and shallow lakes cover a large part of the region, this work will give important information on the distribution and stratigraphy of sedimentary deposits not included in the regular mapping of unconsolidated Quaternary deposits within the site investigation programme. Samples were also collected for laboratory analyses of grain size distribution, mineralogical composition as well as the total content of C, N and S and calcium carbonate. The analyses will be carried out on selected samples of representative sedimentary units in order to characterise the chemical and physical properties of the unconsolidated deposits. The analytical data will be useful for the hydrogeological modelling and for models of the Quaternary evolution of the area. The mineralogical analyses of clay may provide information on the origin of the clay particles. One stratigraphic sequence from Lake Eckarfjaerden will be stored for later analyses, e.g. pollen analysis. This report includes field data from spring 2003. Together, the field data and the forthcoming results from the laboratory analyses will form the basis for construction of stratigraphical profiles to be presented in a following report in the fall 2003.

Forsmark site investigation. Investigation of marine and lacustrine sediment in lakes. Field data 2003

International Nuclear Information System (INIS)

Hedenstroem, Anna

2003-04-01

The aim of this activity is to describe the aerial and stratigraphical distribution of marine and lacustrine sediment i.e. sediment overlaying the glacial till and/or bedrock surface, in lakes in the Forsmark area. The investigation is carried out within areas where mapping of unconsolidated Quaternary deposits is presently carried out. Since small and shallow lakes cover a large part of the region, this work will give important information on the distribution and stratigraphy of sedimentary deposits not included in the regular mapping of unconsolidated Quaternary deposits within the site investigation programme. Samples were also collected for laboratory analyses of grain size distribution, mineralogical composition as well as the total content of C, N and S and calcium carbonate. The analyses will be carried out on selected samples of representative sedimentary units in order to characterise the chemical and physical properties of the unconsolidated deposits. The analytical data will be useful for the hydrogeological modelling and for models of the Quaternary evolution of the area. The mineralogical analyses of clay may provide information on the origin of the clay particles. One stratigraphic sequence from Lake Eckarfjaerden will be stored for later analyses, e.g. pollen analysis. This report includes field data from spring 2003. Together, the field data and the forthcoming results from the laboratory analyses will form the basis for construction of stratigraphical profiles to be presented in a following report in the fall 2003

Investigation and feasibility study of a former manufactured gas plant site in Tuttlingen (Germany), based on individually determined clean-up criteria

Energy Technology Data Exchange (ETDEWEB)

Heinecker, C.; Pickel, H.-J.; Duffek, J. [HPC Harress Pickel Consult GmbH, Fuldatal (Germany)

1995-12-31

At the request of the former plant operator, a manufactured gas plant site in Tuttlingen, Germany, was investigated from 1988 through 1992 for subsurface soil contamination resulting from former activities. In 1991, the contents of the former tar pits and parts of the adjacent soil contaminations were removed in the course of clean-up activities by means of excavation and disposed at a special waste site. Following an initial risk assessment, a remedial investigation was carried out in order to further delineate the contaminated areas as well as to create a reliable database for a feasibility study of remedial alternatives. The feasibility study followed applicable Baden-Wurttemberg state guidelines, including the following elements: Determination of the clean-up goals for soils; pre-selection of the clean-up procedure; cost estimate; cost-effectiveness study; Non-monetary evaluation; and total evaluation/clean-up proposal. The following general alternatives were available for the definition of clean-up goals: background values (`H-values`); general guidelines values (`SZ-values`); and clean-up goals based on contaminant fate and transport as well as site use (`SZA-values`).

Coordination Environment of Copper Sites in Cu-CHA Zeolite Investigated by Electron Paramagnetic Resonance

DEFF Research Database (Denmark)

Godiksen, Anita; Stappen, Frederick N.; Vennestrøm, Peter N. R.

2014-01-01

Cu-CHA combines high activity for the selective catalytic reduction (SCR) reaction with better hydrothermal stability and selectivity compared to other copper-substituted zeolites. At the same time Cu-CHA offers an opportunity for unraveling the coordination environment of the copper centers since...... the zeolite framework is very simple with only one crystallographically independent tetrahedral site (T-site). In this study the results of an X-band electron paramagnetic resonance (EPR) investigation of ion-exchanged Cu-CHA zeolite with a Si/Al ratio of 14 ± 1 is presented. Different dehydration treatments...... of the EPR silent monomeric Cu2+ in copper-substituted zeolites is suggested to be copper species with an approximate trigonal coordination sphere appearing during the dehydration. After complete dehydration at 250 °C the majority of the EPR silent Cu2+ is suggested to exist as Cu2+–OH– coordinated to two...

The mechanisms of substrates interaction with the active site of Mycobacterium tuberculosis tyrosyl-tRNA synthetase studied by molecular dynamics simulations

Directory of Open Access Journals (Sweden)

Mykuliak V. V.

2014-03-01

Full Text Available Aim. To study the mechanisms of substrates interaction with the active site of Mycobacterium tuberculosis tyrosyl-tRNA synthetase (MtTyrRS. Methods. Complexes of MtTyrRS with tyrosine, ATP and tyrosyl adenylate were constructed by superposition of the MtTyrRS structure and crystallographic structures of bacterial TyrRS. All complexes of MtTyrRS with substrates were investigated by molecular dynamics (MD simulations in solution. Results. It was shown the formation of network of hydrogen bonds between substrates and the MtTyrRS active center, which were stable in the course of MD simulations. ATP binds in the active site both by hydrogen bonds and via electrostatic interactions with Lys231 and Lys234 of catalytic KFGKS motif. Conclusions. The L-tyrosine binding site in the enzyme active site is negatively charged, whereas the ATP binding site contains positive Lys231 and Lys234 residues of catalytic KFGKS motif. The occupancy of H-bonds between substrates and the enzyme evidences a significant conformational mobility of the active site.

Theoretical investigation of active sites at the corners of Mgcl 2 crystallites in supported ziegler-natta catalysts

KAUST Repository

Correa, Andrea

2012-05-08

We present a theoretical study on possible models of catalytic active species corresponding to Ti-chloride species adsorbed at the corners of MgCl 2 crystallites. First we focused our efforts on the interaction between prototypes of three industrially relevant Lewis bases used as internal donors (1,3-diethers, alkoxysilanes and succinates) and MgCl 2 units at the corner of a MgCl 2 crystallite. Our calculations show that the energetic cost to extract MgCl 2 units at the corner of (104) edged MgCl 2 crystallites is not prohibitive, and that Lewis bases added during catalyst preparation make this process easier. After removal of one MgCl 2 unit, a short (110) stretch joining the (104) edges is formed. Adsorption of TiCl 4 on the generated vacancy originates a Ti-active species. In the second part of this manuscript, we report on the stereo- and regioselective behavior of this model of active species in the absence as well as in the presence of the three Lewis bases indicated above. Surface reconstruction due to the additional adsorption of an extra MgCl 2 layer is also considered. We show that, according to experimental data, Lewis bases coordinated in the proximity of the active Ti center confer a remarkable stereoselectivity. Moreover, surface reconstruction as well as donor coordination would improve regioselectivity by disfavoring secondary propene insertion. While still models of possible active species, our results indicate that defects, corners and surface reconstruction should be considered as possible anchoring sites for the catalytically active Ti-species. © 2012 American Chemical Society.

Remedial investigation/feasibility study work plan for the 300-FF-1 operable unit, Hanford Site, Richland, Washington

International Nuclear Information System (INIS)

1990-03-01

Over 1,400 waste facilities have been identified on the Hanford Site. Most of the waste facilities are located within geographic areas on the Hanford Site that are referred to as the 100, 200, 300, 400, and 1100 areas. The purpose of this work plan is to document the project scoping process and to outline all remedial investigation/feasibility study (RI/FS) activities, to determine the nature and extent of the threat presented by releases of hazardous substances from the operable unit, and to evaluate proposed remedies for such releases. The goal of the 300-FF-1 remedial investigation (RI) is to provide sufficient information needed to conduct the feasibility study (FS), by determining the nature and extent of the threat to public health and the environment posed by releases of hazardous substances from 300-FF-1, and the performance of specific remedial technologies. 62 refs., 28 figs., 48 tabs

Disulfide bond within mu-calpain active site inhibits activity and autolysis.

Science.gov (United States)

Lametsch, René; Lonergan, Steven; Huff-Lonergan, Elisabeth

2008-09-01

Oxidative processes have the ability to influence mu-calpain activity. In the present study the influence of oxidation on activity and autolysis of mu-calpain was examined. Furthermore, LC-MS/MS analysis was employed to identify and characterize protein modifications caused by oxidation. The results revealed that the activity of mu-calpain is diminished by oxidation with H2O2 in a reversible manner involving cysteine and that the rate of autolysis of mu-calpain concomitantly slowed. The LC-MS/MS analysis of the oxidized mu-calpain revealed that the amino acid residues 105-133 contained a disulfide bond between Cys(108) and Cys(115). The finding that the active site cysteine in mu-calpain is able to form a disulfide bond has, to our knowledge, not been reported before. This could be part of a unique oxidation mechanism for mu-calpain. The results also showed that the formation of the disulfide bond is limited in the control (no oxidant added), and further limited in a concentration-dependent manner when beta-mercaptoethanol is added. However, the disulfide bond is still present to some extent in all conditions indicating that the active site cysteine is potentially highly susceptible to the formation of this intramolecular disulfide bond.

Prediction of P53 mutants (multiple sites transcriptional activity based on structural (2D&3D properties.

Directory of Open Access Journals (Sweden)

R Geetha Ramani

Full Text Available Prediction of secondary site mutations that reinstate mutated p53 to normalcy has been the focus of intense research in the recent past owing to the fact that p53 mutants have been implicated in more than half of all human cancers and restoration of p53 causes tumor regression. However laboratory investigations are more often laborious and resource intensive but computational techniques could well surmount these drawbacks. In view of this, we formulated a novel approach utilizing computational techniques to predict the transcriptional activity of multiple site (one-site to five-site p53 mutants. The optimal MCC obtained by the proposed approach on prediction of one-site, two-site, three-site, four-site and five-site mutants were 0.775,0.341,0.784,0.916 and 0.655 respectively, the highest reported thus far in literature. We have also demonstrated that 2D and 3D features generate higher prediction accuracy of p53 activity and our findings revealed the optimal results for prediction of p53 status, reported till date. We believe detection of the secondary site mutations that suppress tumor growth may facilitate better understanding of the relationship between p53 structure and function and further knowledge on the molecular mechanisms and biological activity of p53, a targeted source for cancer therapy. We expect that our prediction methods and reported results may provide useful insights on p53 functional mechanisms and generate more avenues for utilizing computational techniques in biological data analysis.

HTO and OBT activity concentrations in soil at the historical atmospheric HT release site (Chalk River Laboratories)

International Nuclear Information System (INIS)

Kim, S.B.; Bredlaw, M.; Korolevych, V.Y.

2012-01-01

Tritium is routinely released by the Chalk River Laboratories (CRL) nuclear facilities. Three International HT release experiments have been conducted at the CRL site in the past. The site has not been disturbed since the last historical atmospheric testing in 1994 and presents an opportunity to assess the retention of tritium in soil. This study is devoted to the measurement of HTO and OBT activity concentration profiles in the subsurface 25 cm of soil. In terms of soil HTO, there is no evidence from the past HT release experiments that HTO was retained. The HTO activity concentration in the soil pore water appears similar to concentrations found in background areas in Ontario. In contrast, OBT activity concentrations in soil at the same site were significantly higher than HTO activity concentrations in soil. Elevated OBT appears to reside in the top layer of the soil (0–5 cm). In addition, OBT activity concentrations in the top soil layer did not fluctuate much with season, again, quite in contrast with soil HTO. This result suggests that OBT activity concentrations retained the signature of the historical tritium releases. Highlights: ► At the historical HT release site, HTO and OBT activity concentrations in soil depths were investigated. ► Most organically bound tritium exists in the top layer of the soil. ► The results indicated that OBT activity concentrations can be reflective of historical tritium releases into the environment.

Influenza B viruses with mutation in the neuraminidase active site, North Carolina, USA, 2010-11.

Science.gov (United States)

Sleeman, Katrina; Sheu, Tiffany G; Moore, Zack; Kilpatrick, Susan; Garg, Shikha; Fry, Alicia M; Gubareva, Larisa V

2011-11-01

Oseltamivir is 1 of 2 antiviral medications available for the treatment of influenza B virus infections. We describe and characterize a cluster of influenza B viruses circulating in North Carolina with a mutation in the neuraminidase active site that may reduce susceptibility to oseltamivir and the investigational drug peramivir but not to zanamivir.

Active catalytic sites in the ammoxidation of propane and propene over V-Sb-O catalysts

Energy Technology Data Exchange (ETDEWEB)

Buchholz, S.A.; Zanthoff, H.W. [Bochum Univ. (Germany). Lehrstuhl fuer Technische Chemie

1998-12-31

The ammoxidation of propane over VSb{sub y}O{sub x} catalysts (y=1, 2, 5) was investigated with respect to the role of different oxygen species in the selective and non selective reaction steps using transient experiments in the Temporal Analysis of Products (TAP) reactor. Only lattice oxygen is involved in the oxidation reactions. Using isotopic labelled oxygen it is shown that two different active sites exist on the surface. On site A, which can be reoxidized faster by gas phase oxygen compared to site B, mainly CO is formed. On site B CO{sub 2} and acrolein as well as NO and N{sub 2}O in the presence of ammonia in the feed gas are formed and reoxidation mainly occurs with bulk lattice oxygen. (orig.)

Anisotropic Covalency Contributions to Superexchange Pathways in Type One Copper Active Sites

Science.gov (United States)

2015-01-01

Type one (T1) Cu sites deliver electrons to catalytic Cu active sites: the mononuclear type two (T2) Cu site in nitrite reductases (NiRs) and the trinuclear Cu cluster in the multicopper oxidases (MCOs). The T1 Cu and the remote catalytic sites are connected via a Cys-His intramolecular electron-transfer (ET) bridge, which contains two potential ET pathways: P1 through the protein backbone and P2 through the H-bond between the Cys and the His. The high covalency of the T1 Cu–S(Cys) bond is shown here to activate the T1 Cu site for hole superexchange via occupied valence orbitals of the bridge. This covalency-activated electronic coupling (HDA) facilitates long-range ET through both pathways. These pathways can be selectively activated depending on the geometric and electronic structure of the T1 Cu site and thus the anisotropic covalency of the T1 Cu–S(Cys) bond. In NiRs, blue (π-type) T1 sites utilize P1 and green (σ-type) T1 sites utilize P2, with P2 being more efficient. Comparing the MCOs to NiRs, the second-sphere environment changes the conformation of the Cys-His pathway, which selectively activates HDA for superexchange by blue π sites for efficient turnover in catalysis. These studies show that a given protein bridge, here Cys-His, provides different superexchange pathways and electronic couplings depending on the anisotropic covalencies of the donor and acceptor metal sites. PMID:25310460

Corrective Action Investigation Plan for Corrective Action Unit 545: Dumps, Waste Disposal Sites, and Buried Radioactive Materials Nevada Test Site, Nevada

International Nuclear Information System (INIS)

Alfred Wickline

2007-01-01

Corrective Action Unit 545, Dumps, Waste Disposal Sites, and Buried Radioactive Materials, consists of seven inactive sites located in the Yucca Flat area and one inactive site in the Pahute Mesa area. The eight CAU 545 sites consist of craters used for mud disposal, surface or buried waste disposed within craters or potential crater areas, and sites where surface or buried waste was disposed. The CAU 545 sites were used to support nuclear testing conducted in the Yucca Flat area during the 1950s through the early 1990s, and in Area 20 in the mid-1970s. This Corrective Action Investigation Plan has been developed in accordance with the Federal Facility Agreement and Consent Order that was agreed to by the State of Nevada, the U.S. Department of Energy, and the U.S. Department of Defense. Under the Federal Facility Agreement and Consent Order, this Corrective Action Investigation Plan will be submitted to the Nevada Division of Environmental Protection for approval. Fieldwork will be conducted following approval

Improving Sampling, Analysis, and Data Management for Site Investigation and Cleanup

Science.gov (United States)

The United States Environmental Protection Agency (EPA) supports the adoption of streamlined approaches to sampling, analysis, and data management activities conducted during site assessment, characterization, and cleanup.

Site characterization quality assurance for the California LLRW Disposal Site Project

International Nuclear Information System (INIS)

Hanrahan, T.P.; Ench, J.E.; Serlin, C.L.; Bennett, C.B.

1988-01-01

In December of 1985 US Ecology was chosen as the license designee for the State of California's low-level radioactive waste disposal facility. In early 1987, three candidate sites were selected for characterization studies in preparation for identifying the preferred site. The geotechnical characterization activities along with studies of the ecological and archaeological attributes, as well as assessments of the socio-economic impacts and cultural resources all provide input towards selection of the proposed site. These technical studies in conjunction with comments from local citizen committees and other interested parties are used as a basis for determining the proposed site for which full site characterization as required by California licensing requirements are undertaken. The purpose of this paper is to present an overview of the program for Quality Assurance and Quality Control for the site characterization activities on the California LLRW Disposal Site Project. The focus is on three major perspectives: The composite QA Program and two of the primary characterization activities, the geotechnical and the meteorological investigations

Site characterization techniques used in environmental remediation activities

International Nuclear Information System (INIS)

Kostelnik, K.M.

2000-01-01

As a result of decades of nuclear energy research, weapons production, as well as ongoing operations, a significant amount of radioactive contamination has occurred throughout the United States Department of Energy (DOE) complex. DOE facility are in the process of assessing and potentially remediating various sites according to the regulations imposed by a Federal Facility Agreement and Consent order (FFA/CO) between DOE, the state in which the facility is located, and the U.S. Environmental Protection Agency (EPA). In support of these active site remediation efforts, the DOE has devoted considerable resources towards the development of innovative site characterization techniques that support environmental restoration activities. These resources and efforts have focused on various aspects of this complex problem. Research and technology development conducted at the Idaho National Engineering and Environmental Laboratory (INEEL) has resulted in the ability and state-of-the-art equipment required to obtain real-time, densely spaced, in situ characterization data (i.e. detection, speciation, and location) of various radionuclides and contaminants. The Remedial Action Monitoring System (RAMS), developed by the INEEL, consists of enhanced sensor technology, measurement modeling and interpretation techniques, and a suite of deployment platforms which can be interchanged to directly support remedial cleanup and site verification operations. In situ characterization techniques have advanced to the point where they are being actively deployed in support of remedial operations. The INEEL has deployed its system at various DOE and international sites. The deployment of in situ characterization systems during environmental restoration operations has shown that this approach results in several significant benefits versus conventional sampling techniques. A flexible characterization system permits rapid modification to satisfy physical site conditions, available site resources

Stereospecific suppression of active site mutants by methylphosphonate substituted substrates reveals the stereochemical course of site-specific DNA recombination

OpenAIRE

Rowley, Paul A.; Kachroo, Aashiq H.; Ma, Chien-Hui; Maciaszek, Anna D.; Guga, Piotr; Jayaram, Makkuni

2015-01-01

Tyrosine site-specific recombinases, which promote one class of biologically important phosphoryl transfer reactions in DNA, exemplify active site mechanisms for stabilizing the phosphate transition state. A highly conserved arginine duo (Arg-I; Arg-II) of the recombinase active site plays a crucial role in this function. Cre and Flp recombinase mutants lacking either arginine can be rescued by compensatory charge neutralization of the scissile phosphate via methylphosphonate (MeP) modificati...

Corrective Action Investigation Plan for Corrective Action Unit 576: Miscellaneous Radiological Sites and Debris Nevada National Security Site, Nevada, Revision 0

Energy Technology Data Exchange (ETDEWEB)

Matthews, Patrick [Navarro, Las Vegas, NV (United States)

2016-12-01

Corrective Action Unit (CAU) 576 is located in Areas 2, 3, 5, 8, and 9 of the Nevada National Security Site, which is approximately 65 miles northwest of Las Vegas, Nevada. CAU 576 is a grouping of sites where there has been a suspected release of contamination associated with nuclear testing. This document describes the planned investigation of CAU 576, which comprises the following corrective action sites (CASs): 00-99-01, Potential Source Material; 02-99-12, U-2af (Kennebec) Surface Rad-Chem Piping; 03-99-20, Area 3 Subsurface Rad-Chem Piping; 05-19-04, Frenchman Flat Rad Waste Dump ; 09-99-08, U-9x (Allegheny) Subsurface Rad-Chem Piping; 09-99-09, U-9its u24 (Avens-Alkermes) Surface Contaminated Flex Line These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the Corrective Action Decision Document (CADD).

Active sites in char gasification: Final technical report

Energy Technology Data Exchange (ETDEWEB)

Wojtowicz, M.; Lilly, W.D.; Perkins, M.T.; Hradil, G.; Calo, J.M.; Suuberg, E.M.

1987-09-01

Among the key variables in the design of gasifiers and combustors is the reactivity of the chars which must be gasified or combusted. Significant loss of unburned char is unacceptable in virtually any process; the provision of sufficient residence time for complete conversion is essential. A very wide range of reactivities are observed, depending upon the nature of the char in a process. The current work focuses on furthering the understanding of gasification reactivities of chars. It has been well established that the reactivity of char to gasification generally depends upon three principal factors: (1) the concentration of ''active sites'' in the char; (2) mass transfer within the char; and (3) the type and concentration of catalytic impurities in the char. The present study primarily addresses the first factor. The subject of this research is the origin, nature, and fate of active sites in chars derived from parent hydrocarbons with coal-like structure. The nature and number of the active sites and their reactivity towards oxygen are examined in ''model'' chars derived from phenol-formaldehyde type resins. How the active sites are lost by the process of thermal annealing during heat treatment of chars are studied, and actual rate for the annealing process is derived. Since intrinsic char reactivities are of primary interest in the present study, a fair amount of attention was given to the model char synthesis and handling so that the effect of catalytic impurities and oxygen-containing functional groups in the chemical structure of the material were minimized, if not completely eliminated. The project would not be considered complete without comparing characteristic features of synthetic chars with kinetic behavior exhibited by natural chars, including coal chars.

Remedial investigation for the 200-BP-1 operable unit, Hanford Site, Richland, Washington

International Nuclear Information System (INIS)

Buckmaster, M.A.

1991-01-01

The Hanford Site, Richland, Washington, contains over 1500 identified waste sites that will be characterized and remediated over the next 30 years. In support of the ''Hanford Federal Facility Agreement and Consent Order,'' the US Department of Energy has initiated a remedial investigation/feasibility study (RI/FS) at the 200-BP-1 operable unit. The 200-BP-1 RI is the first Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) investigation on the Hanford Site that involves drilling into highly radioactive and chemically contaminated soils. The initial phase of the site characterization is oriented toward determining the nature and extent of any contamination present in the vicinity of the 200-BP-1 operable unit. The major focus of the Phase I RI is the drilling and sampling of 10 inactive waste disposal units which received low level radioactive liquid waste

Enhancement in catalytic activity of Aspergillus niger XynB by selective site-directed mutagenesis of active site amino acids.

Science.gov (United States)

Wu, Xiuyun; Tian, Zhennan; Jiang, Xukai; Zhang, Qun; Wang, Lushan

2018-01-01

XynB from Aspergillus niger ATCC1015 (AnXynB) is a mesophilic glycoside hydrolase (GH) family 11 xylanase which holds great potentials in a wide variety of industrial applications. In the present study, the catalytic activity and stability of AnXynB were improved by a combination of computational and experimental approaches. Virtual mutation and molecular dynamics simulations indicated that the introduction of Glu and Asn altered the interaction network at the - 3 subsite. Interestingly, the double mutant S41N/T43E displayed 72% increase in catalytic activity when compared to the wild type (WT). In addition, it also showed a better thermostability than the WT enzyme. Kinetic determination of the T43E and S41N/T43E mutants suggested that the higher xylanase activity is probably due to the increasing binding affinity of enzyme and substrate. Consequently, the enzyme activity and thermostability of AnXynB was both increased by selective site-directed mutagenesis at the - 3 subsite of its active site architecture which provides a good example for a successfully engineered enzyme for potential industrial application. Moreover, the molecular evolution approach adopted in this study led to the design of a library of sequences that captures a meaningful functional diversity in a limited number of protein variants.

Animal Investigation Program: Nevada Test Site and Vicinity. Annual report, 1979

International Nuclear Information System (INIS)

Smith, D.D.; Giles, Jr.; Bernhardt, D.E.

1981-05-01

Data are presented from the radioanalyses of tissues collected from cattle, mule deer, desert bighorn sheep, rabbits, chukar, golden eagles, and other wildlife that resided on or near the Nevada Test Site during 1979. Routine and special activities of the Animal Investigation Program are also discussed. Other than the naturally occurring potassium-40, gamma-emitting radionuclides were detected infrequently. Strontium-90 concentrations in bones from deer, cattle, and desert bighorn sheep were lower than those of recent years. Tritium concentrations were generally within expected environmental limits with the exception of animals exposed to known sources of contamination; e.g., drainage ponds from Area 12 tunnels or the Sedan Crater. Plutonium levels in all tissues from all species showed little variation to those levels in samples collected in recent years. Radionuclide tissue concentrations were generally higher in the tissues of animals residing in Area 15 than in similar animals collected from other Nevada Test Site areas. Hypothetical annual dose estimates to man were calculated on the basis of the daily consumption of 0.5 kilogra of liver or muscle from animals that contained peak radionuclide levels. The movements of 25 mule deer outfitted with collars containing a radio transmitter unit were monitored on a weekly basis. No gross or microscopic lesions were found in necropsied animals that could be directly attributed to the effects of ionizing radiation

Corrective Action Investigation Plan for Corrective Action Unit 561: Waste Disposal Areas, Nevada Test Site, Nevada, Revision 0

International Nuclear Information System (INIS)

Grant Evenson

2008-01-01

Corrective Action Unit (CAU) 561 is located in Areas 1, 2, 3, 5, 12, 22, 23, and 25 of the Nevada Test Site, which is approximately 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 561 is comprised of the 10 corrective action sites (CASs) listed below: (1) 01-19-01, Waste Dump; (2) 02-08-02, Waste Dump and Burn Area; (3) 03-19-02, Debris Pile; (4) 05-62-01, Radioactive Gravel Pile; (5) 12-23-09, Radioactive Waste Dump; (6) 22-19-06, Buried Waste Disposal Site; (7) 23-21-04, Waste Disposal Trenches; (8) 25-08-02, Waste Dump; (9) 25-23-21, Radioactive Waste Dump; and (10) 25-25-19, Hydrocarbon Stains and Trench. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on April 28, 2008, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and National Security Technologies, LLC. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 561. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to each CAS. The scope of the Corrective Action Investigation for CAU 561 includes the following activities: (1) Move surface debris and/or materials, as needed, to facilitate sampling. (2) Conduct

Site-specific RNase A activity was dramatically reduced in serum from multiple types of cancer patients.

Directory of Open Access Journals (Sweden)

Weiyan Huang

Full Text Available Potent RNase activities were found in the serum of mammals but the physiological function of the RNases was never well illustrated, largely due to the caveats in methods of RNase activity measurement. None of the existing methods can distinguish between RNases with different target specificities. A systematic study was recently carried out in our lab to investigate the site-specificity of serum RNases on double-stranded RNA substrates, and found that serum RNases cleave double-stranded RNAs predominantly at 5'-U/A-3' and 5'-C/A-3' dinucleotide sites, in a manner closely resembling RNase A. Based on this finding, a FRET assay was developed in the current study to measure this site-specific serum RNase activity in human samples using a double stranded RNA substrate. We demonstrated that the method has a dynamic range of 10(-5 mg/ml- 10(-1 mg/ml using serial dilution of RNase A. The sera of 303 cancer patients were subjected to comparison with 128 healthy controls, and it was found that serum RNase activities visualized with this site-specific double stranded probe were found to be significantly reduced in patients with gastric cancer, liver cancer, pancreatic cancer, esophageal cancer, ovary cancer, cervical cancer, bladder cancer, kidney cancer and lung cancer, while only minor changes were found in breast and colon cancer patients. This is the first report using double stranded RNA as probe to quantify site-specific activities of RNase A in a serum. The results illustrated that RNase A might be further evaluated to determine if it can serve as a new class of biomarkers for certain cancer types.

Site-Specific RNase A Activity Was Dramatically Reduced in Serum from Multiple Types of Cancer Patients

Science.gov (United States)

Huang, Weiyan; Zhao, Mei; Wei, Na; Wang, Xiaoxia; Cao, Huqing; Du, Quan; Liang, Zicai

2014-01-01

Potent RNase activities were found in the serum of mammals but the physiological function of the RNases was never well illustrated, largely due to the caveats in methods of RNase activity measurement. None of the existing methods can distinguish between RNases with different target specificities. A systematic study was recently carried out in our lab to investigate the site-specificity of serum RNases on double-stranded RNA substrates, and found that serum RNases cleave double-stranded RNAs predominantly at 5′-U/A-3′ and 5′-C/A-3′ dinucleotide sites, in a manner closely resembling RNase A. Based on this finding, a FRET assay was developed in the current study to measure this site-specific serum RNase activity in human samples using a double stranded RNA substrate. We demonstrated that the method has a dynamic range of 10−5 mg/ml- 10−1 mg/ml using serial dilution of RNase A. The sera of 303 cancer patients were subjected to comparison with 128 healthy controls, and it was found that serum RNase activities visualized with this site-specific double stranded probe were found to be significantly reduced in patients with gastric cancer, liver cancer, pancreatic cancer, esophageal cancer, ovary cancer, cervical cancer, bladder cancer, kidney cancer and lung cancer, while only minor changes were found in breast and colon cancer patients. This is the first report using double stranded RNA as probe to quantify site-specific activities of RNase A in a serum. The results illustrated that RNase A might be further evaluated to determine if it can serve as a new class of biomarkers for certain cancer types. PMID:24805924

Corrective Action Investigation Plan for Corrective Action Unit 528: Polychlorinated Biphenyls Contamination, Nevada Test Site, Nevada, Rev. 0

Energy Technology Data Exchange (ETDEWEB)

U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office

2003-05-08

This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 528, Polychlorinated Biphenyls Contamination (PCBs), Nevada Test Site (NTS), Nevada, under the Federal Facility Agreement and Consent Order. Located in the southwestern portion of Area 25 on the NTS in Jackass Flats (adjacent to Test Cell C [TCC]), CAU 528 consists of Corrective Action Site 25-27-03, Polychlorinated Biphenyls Surface Contamination. Test Cell C was built to support the Nuclear Rocket Development Station (operational between 1959 and 1973) activities including conducting ground tests and static firings of nuclear engine reactors. Although CAU 528 was not considered as a direct potential source of PCBs and petroleum contamination, two potential sources of contamination have nevertheless been identified from an unknown source in concentrations that could potentially pose an unacceptable risk to human health and/or the environment. This CAU's close proximity to TCC prompted Shaw to collect surface soil samples, which have indicated the presence of PCBs extending throughout the area to the north, east, south, and even to the edge of the western boundary. Based on this information, more extensive field investigation activities are being planned, the results of which are to be used to support a defensible evaluation of corrective action alternatives in the corrective action decision document.

Mapping the active site of vaccinia virus RNA triphosphatase

International Nuclear Information System (INIS)

Gong Chunling; Shuman, Stewart

2003-01-01

The RNA triphosphatase component of vaccinia virus mRNA capping enzyme (the product of the viral D1 gene) belongs to a family of metal-dependent phosphohydrolases that includes the RNA triphosphatases of fungi, protozoa, Chlorella virus, and baculoviruses. The family is defined by two glutamate-containing motifs (A and C) that form the metal-binding site. Most of the family members resemble the fungal and Chlorella virus enzymes, which have a complex active site located within the hydrophilic interior of a topologically closed eight-stranded β barrel (the so-called ''triphosphate tunnel''). Here we queried whether vaccinia virus capping enzyme is a member of the tunnel subfamily, via mutational mapping of amino acids required for vaccinia triphosphatase activity. We identified four new essential side chains in vaccinia D1 via alanine scanning and illuminated structure-activity relationships by conservative substitutions. Our results, together with previous mutational data, highlight a constellation of six acidic and three basic amino acids that likely compose the vaccinia triphosphatase active site (Glu37, Glu39, Arg77, Lys107, Glu126, Asp159, Lys161, Glu192, and Glu194). These nine essential residues are conserved in all vertebrate and invertebrate poxvirus RNA capping enzymes. We discerned no pattern of clustering of the catalytic residues of the poxvirus triphosphatase that would suggest structural similarity to the tunnel proteins (exclusive of motifs A and C). We infer that the poxvirus triphosphatases are a distinct lineage within the metal-dependent RNA triphosphatase family. Their unique active site, which is completely different from that of the host cell's capping enzyme, recommends the poxvirus RNA triphosphatase as a molecular target for antipoxviral drug discovery

SABER: a computational method for identifying active sites for new reactions.

Science.gov (United States)

Nosrati, Geoffrey R; Houk, K N

2012-05-01

A software suite, SABER (Selection of Active/Binding sites for Enzyme Redesign), has been developed for the analysis of atomic geometries in protein structures, using a geometric hashing algorithm (Barker and Thornton, Bioinformatics 2003;19:1644-1649). SABER is used to explore the Protein Data Bank (PDB) to locate proteins with a specific 3D arrangement of catalytic groups to identify active sites that might be redesigned to catalyze new reactions. As a proof-of-principle test, SABER was used to identify enzymes that have the same catalytic group arrangement present in o-succinyl benzoate synthase (OSBS). Among the highest-scoring scaffolds identified by the SABER search for enzymes with the same catalytic group arrangement as OSBS were L-Ala D/L-Glu epimerase (AEE) and muconate lactonizing enzyme II (MLE), both of which have been redesigned to become effective OSBS catalysts, demonstrated by experiments. Next, we used SABER to search for naturally existing active sites in the PDB with catalytic groups similar to those present in the designed Kemp elimination enzyme KE07. From over 2000 geometric matches to the KE07 active site, SABER identified 23 matches that corresponded to residues from known active sites. The best of these matches, with a 0.28 Å catalytic atom RMSD to KE07, was then redesigned to be compatible with the Kemp elimination using RosettaDesign. We also used SABER to search for potential Kemp eliminases using a theozyme predicted to provide a greater rate acceleration than the active site of KE07, and used Rosetta to create a design based on the proteins identified. Copyright © 2012 The Protein Society.

Perspectives of investigation and development of Semipalatinsk Test Site

International Nuclear Information System (INIS)

Kadyrzhanov, K.K.; Lukashenko, S.N.

2008-01-01

Full text: Since the Semipalatinsk Test Site has been stopped and up until now, National Nuclear Center of the Republic of Kazakhstan (NNC RK) in cooperation with other specialist from Kazakhstan and international scientific community have accumulated large scope of information about current radiological situation at Semipalatinsk Nuclear Test Site (SNTS) and adjacent territories. There were revealed all important spots of radioactive contamination, identified main pathways and mechanisms for present and potential proliferation of radioactive substances. Obtained data assure us that present-day SNTS provides no negative impact on population on adjacent to the Site territories excluding people in the water basin of the river Shagan. Compliance with regulatory requirements and special rules for SNTS territory assures radiation safety at commercial activities on the Site. At the same time, the radiological situation does not remain stable; there were revealed the processes of radionuclide migration what requires regular monitoring of radiological situation at SNTS. Taking into account the scale of the Site and the variety of tests performed there, the information available about SNTS can not be completely exhaustive but enables us to propose a scientifically grounded plan for further research and practical measures aimed at remediation and reclamation of lands. implementation of such measures should return up to 80% of the lands to commercial use. SNTS is one of the world largest nuclear test sites with decisive contribution to creation and development of nuclear weapon. To considerable extent, these were works at SNTS which established nuclear parity between the superpowers one of the crucial factors in the history of human civilization in the 20 century. Also taking into account the interest to SNTS paid by international organizations, it is reasonable to initiate a procedure and recognize SNTS as a landmark including it in the UNESCO List of Cultural and Nature

GASS-WEB: a web server for identifying enzyme active sites based on genetic algorithms.

Science.gov (United States)

Moraes, João P A; Pappa, Gisele L; Pires, Douglas E V; Izidoro, Sandro C

2017-07-03

Enzyme active sites are important and conserved functional regions of proteins whose identification can be an invaluable step toward protein function prediction. Most of the existing methods for this task are based on active site similarity and present limitations including performing only exact matches on template residues, template size restraints, despite not being capable of finding inter-domain active sites. To fill this gap, we proposed GASS-WEB, a user-friendly web server that uses GASS (Genetic Active Site Search), a method based on an evolutionary algorithm to search for similar active sites in proteins. GASS-WEB can be used under two different scenarios: (i) given a protein of interest, to match a set of specific active site templates; or (ii) given an active site template, looking for it in a database of protein structures. The method has shown to be very effective on a range of experiments and was able to correctly identify >90% of the catalogued active sites from the Catalytic Site Atlas. It also managed to achieve a Matthew correlation coefficient of 0.63 using the Critical Assessment of protein Structure Prediction (CASP 10) dataset. In our analysis, GASS was ranking fourth among 18 methods. GASS-WEB is freely available at http://gass.unifei.edu.br/. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

2015 Advanced Site Investigation and Monitoring Report Riverton, Wyoming, Processing Site September 2016

Energy Technology Data Exchange (ETDEWEB)

Frazier, William [U.S. Dept. of Energy, Washington, DC (United States). Office of Legacy Management (LM); Campbell, Sam [Navarro Research and Engineering, Inc., Oak Ridge, TN (United States)

2016-09-01

The U.S. Department of Energy conducted initial groundwater characterization of the Riverton, Wyoming, Processing Site in the 1990s. The characterization culminated in a Site Observational Work Plan in 1998 that recommended a natural flushing compliance strategy. Results of verification monitoring indicated that natural flushing was generally progressing as expected until June 2010, when significant increases in contaminant concentrations were measured in several monitoring wells downgradient of the site after the area flooded. In response to the unexpected results following the flood, an enhanced characterization of the surficial aquifer was conducted in 2012, which included installation of 103 boreholes along nine transects with a Geoprobe, collection of 103 water samples and 65 soil samples, laboratory tests on the soil samples, and additional groundwater modeling. This advanced site investigation report summarizes additional investigation in 2015 through the use of backhoe trenching, sonic drilling, multilevel monitoring wells, direct-push drilling, and temporary well points to collect soil and groundwater samples. Additional surface water measurements were made included the installation of a stilling well and the measurement of stream elevation along the Wind River to approximate upgradient groundwater heads. Groundwater sampling included the addition of geochemical constituents and isotopes that have not been sampled in the past to better understand post-flood conditions and the possibility of additional or ongoing contaminant sources. This sampling was performed to (1) better define the contaminant plumes, (2) verify the occurrence of persistent secondary contaminant sources, (3) better understand the reason for the contaminant spikes after a 2010 flood, and (4) assess contaminant plume stagnation near the Little Wind River. This report provides data analyses and interpretations for the 2015 site investigation that addresses these issues and provides

Corrective Action Investigation Plan for Corrective Action Unit 552: Area 12 Muckpile and Ponds, Nevada Test Site, Nevada, Rev. No.: 1 with ROTC 1 and 2

Energy Technology Data Exchange (ETDEWEB)

David A. Strand

2005-01-01

This Corrective Action Investigation Plan (CAIP) contains project-specific information including facility descriptions, environmental sample collection objectives, and criteria for conducting site investigation activities at Corrective Action Unit (CAU) 552: Area 12 Muckpile and Ponds, Nevada Test Site (NTS), Nevada. This CAIP has been developed in accordance with the ''Federal Facility Agreement and Consent Order'' (FFACO) (1996) that was agreed to by the State of Nevada, the U.S. Department of Energy (DOE), and the U.S. Department of Defense. The NTS is approximately 65 miles (mi) northwest of Las Vegas, Nevada (Figure 1-1). Corrective Action Unit 552 is comprised of the one Corrective Action Site which is 12-23-05, Ponds. One additional CAS, 12-06-04, Muckpile (G-Tunnel Muckpile), was removed from this CAU when it was determined that the muckpile is an active site. A modification to the FFACO to remove CAS 12-06-04 was approved by the Nevada Division of Environmental Protection (NDEP) on December 16, 2004. The G-Tunnel ponds were first identified in the 1991 Reynolds Electrical & Engineering Co., Inc. document entitled, ''Nevada Test Site Inventory of Inactive and Abandoned Facilities and Waste Sites'' (REECo, 1991). Corrective Action Unit 552 is being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Therefore, additional information will be obtained by conducting a corrective action investigation (CAI) prior to evaluating and selecting the corrective action alternatives for the site. The CAI will include field inspections, radiological surveys, and sampling of appropriate media. Data will also be obtained to support investigation-derived waste (IDW) disposal and potential future waste management decisions.

Site investigation report for Waste Area Grouping 4 at Oak Ridge National Laboratory. Volume 1, Text: Environmental Restoration Program

International Nuclear Information System (INIS)

1995-08-01

Waste Area Grouping (WAG) 4 is one of 17 WAGs within and associated with Oak Ridge National Laboratory (ORNL). WAG 4 is located south of the main facility along Lagoon Road. WAG 4 consists of three separate areas: Solid Waste Storage Area (SWSA) 4, a shallow-land-burial ground containing radioactive and potentially hazardous wastes; an experimental Pilot Pit Area, which includes a pilot-scale testing pit; and sections of two abandoned underground pipelines used for transporting liquid, low-level, radioactive waste. SWSA 4 is the largest site at WAG 4, covering approximately 23 acres. In the 1950s, SWSA 4 received a variety of low- and high-activity wastes, including transuranic wastes, all buried in trenches and auger holes. Recent surface water data, collected during monitoring of the tributary to White Oak Creek as part of WAG 2 investigations as well as during previous studies conducted at WAG 4, indicate that a significant amount of 90 Sr is being released from the old burial trenches in SWSA 4. This release represents a significant portion of the ORNL off-site risk (DOE 1993). With recent corrective measures the proportion of the release has increased in 1995. A detailed discussion of the site history and previous investigations is presented in the WAG 4 Preliminary Assessment Report, ORNL/ER-271 (Energy Systems 1994b). In an effort to control the sources of the 90 Sr release and to reduce the off-site risk, a site investigation was initiated to pinpoint those trenches that are the most prominent 90 Sr sources

Facebook, Twitter Activities Sites, Location and Students' Interest in Learning

Science.gov (United States)

Igbo, J. N.; Ezenwaji, Ifeyinwa; Ajuziogu, Christiana U.

2018-01-01

This study was carried out to ascertain the influence of social networking sites activities (twitter and Facebook) on secondary school students' interest in learning It also considered the impact of these social networking sites activities on location of the students. Two research questions and two null hypotheses guided the study. Mean and…

River and river-related drainage area parameters for site investigation program

Energy Technology Data Exchange (ETDEWEB)

Blomqvist, P.; Brunberg, A.K. [Uppsala Univ. (Sweden). Dept. of Limnology; Brydsten, L. [Umeaa Univ. (Sweden). Dept. of Ecology and Environmental Science

2001-05-01

In this paper, a number of parameters of importance to a determination of the function of running waters as transport channels for material from the continents to the sea are presented. We have assumed that retention mechanisms of material in the river and in the riparian zone will be covered by special investigations but tried to create a platform for such investigations by quantification of the extension of different main habitats. The choice of parameters has been made so that also the nature conservation value of the river can be preliminary established, and includes a general description of the river type and the inherent ecosystem. The material links directly to that presented in a previous report concerning site investigation programmes for lakes. The parameters have been divided into five groups: 1) The location of the object relative important gradients in the surrounding nature; 2) The river catchment area and its major constituents; 3) The river morphometry; 4) The river ecosystem; 5) Human-induced damages to the river ecosystem. The first two groups, principally based on the climate, hydrology, geology and vegetation of the catchment area, represent parameters that can be used to establish the rarity and representativity of the system, and will in the context of site investigation program be used as a basis for generalisation of the results. The third group, the river morphometry parameters, are standard parameters for the outline of sampling programmes and for calculations of the physical extension of key habitats in the system. The fourth group, the ecosystem of the river, includes physical, chemical and biological parameters required for determination of the influence from the terrestrial ecosystem of the catchment area, nutrient status, distribution of different habitats, and presence of fish in the system. In the context of site investigation program, the parameters in these two groups will be used for budget calculations of the flow of energy and

River and river-related drainage area parameters for site investigation program

International Nuclear Information System (INIS)

Blomqvist, P.; Brunberg, A.K.; Brydsten, L.

2001-05-01

In this paper, a number of parameters of importance to a determination of the function of running waters as transport channels for material from the continents to the sea are presented. We have assumed that retention mechanisms of material in the river and in the riparian zone will be covered by special investigations but tried to create a platform for such investigations by quantification of the extension of different main habitats. The choice of parameters has been made so that also the nature conservation value of the river can be preliminary established, and includes a general description of the river type and the inherent ecosystem. The material links directly to that presented in a previous report concerning site investigation programmes for lakes. The parameters have been divided into five groups: 1) The location of the object relative important gradients in the surrounding nature; 2) The river catchment area and its major constituents; 3) The river morphometry; 4) The river ecosystem; 5) Human-induced damages to the river ecosystem. The first two groups, principally based on the climate, hydrology, geology and vegetation of the catchment area, represent parameters that can be used to establish the rarity and representativity of the system, and will in the context of site investigation program be used as a basis for generalisation of the results. The third group, the river morphometry parameters, are standard parameters for the outline of sampling programmes and for calculations of the physical extension of key habitats in the system. The fourth group, the ecosystem of the river, includes physical, chemical and biological parameters required for determination of the influence from the terrestrial ecosystem of the catchment area, nutrient status, distribution of different habitats, and presence of fish in the system. In the context of site investigation program, the parameters in these two groups will be used for budget calculations of the flow of energy and

DbPTM 3.0: an informative resource for investigating substrate site specificity and functional association of protein post-translational modifications.

Science.gov (United States)

Lu, Cheng-Tsung; Huang, Kai-Yao; Su, Min-Gang; Lee, Tzong-Yi; Bretaña, Neil Arvin; Chang, Wen-Chi; Chen, Yi-Ju; Chen, Yu-Ju; Huang, Hsien-Da

2013-01-01

Protein modification is an extremely important post-translational regulation that adjusts the physical and chemical properties, conformation, stability and activity of a protein; thus altering protein function. Due to the high throughput of mass spectrometry (MS)-based methods in identifying site-specific post-translational modifications (PTMs), dbPTM (http://dbPTM.mbc.nctu.edu.tw/) is updated to integrate experimental PTMs obtained from public resources as well as manually curated MS/MS peptides associated with PTMs from research articles. Version 3.0 of dbPTM aims to be an informative resource for investigating the substrate specificity of PTM sites and functional association of PTMs between substrates and their interacting proteins. In order to investigate the substrate specificity for modification sites, a newly developed statistical method has been applied to identify the significant substrate motifs for each type of PTMs containing sufficient experimental data. According to the data statistics in dbPTM, >60% of PTM sites are located in the functional domains of proteins. It is known that most PTMs can create binding sites for specific protein-interaction domains that work together for cellular function. Thus, this update integrates protein-protein interaction and domain-domain interaction to determine the functional association of PTM sites located in protein-interacting domains. Additionally, the information of structural topologies on transmembrane (TM) proteins is integrated in dbPTM in order to delineate the structural correlation between the reported PTM sites and TM topologies. To facilitate the investigation of PTMs on TM proteins, the PTM substrate sites and the structural topology are graphically represented. Also, literature information related to PTMs, orthologous conservations and substrate motifs of PTMs are also provided in the resource. Finally, this version features an improved web interface to facilitate convenient access to the resource.

Geochemical investigations at Maxey Flats radioactive waste disposal site

International Nuclear Information System (INIS)

Dayal, R.; Pietrzak, R.F.; Clinton, J.

1984-09-01

As part of the NRC efforts to develop a data base on source term characteristics for low level wastes, Brookhaven National Laboratory (BNL) has produced and analyzed a large amount of data on trench leachate chemistry at existing shallow land burial sites. In this report, we present the results of our investigations at the Maxey Flats, Kentucky disposal site. In particular, data on trench leachate chemistry are reviewed and discussed in terms of mechanisms and processes controlling the composition of trench solutes. Particular emphasis is placed on identifying both intra- and extra-trench factors and processes contributing to source term characteristics, modifications, and uncertainties. BNL research on the Maxey Flats disposal site has provided important information not only on the source term characteristics and the factors contributing to uncertainties in the source term but also some generic insights into such geochemical processes and controls as the mechanics of leachate formation, microbial degradation and development of anoxia, organic complexation and radionuclide mobility, redox inversion and modification of the source term, solubility constraints on solute chemistry, mineral authigenesis, corrosion products and radionuclide scavenging, and the role of organic complexants in geochemical partitioning of radionuclides. A knowledge of such processes and controls affecting the geochemical cycling of radionuclides as well as an understanding of the important factors that contribute to variability and uncertainties in the source term is essential for evaluating the performance of waste package and the site, making valid predictions of release for dose calculations, and for planning site performance monitoring as well as remedial actions. 43 references, 47 figures, 30 tables

Using Carbohydrate Interaction Assays to Reveal Novel Binding Sites in Carbohydrate Active Enzymes

DEFF Research Database (Denmark)

Cockburn, Darrell; Wilkens, Casper; Dilokpimol, Adiphol

2016-01-01

Carbohydrate active enzymes often contain auxiliary binding sites located either on independent domains termed carbohydrate binding modules (CBMs) or as so-called surface binding sites (SBSs) on the catalytic module at a certain distance from the active site. The SBSs are usually critical...

Recent drilling program to investigate radionuclide migration at the Nevada Test Site

International Nuclear Information System (INIS)

Smith, D.K.

1997-01-01

Recent drilling affords new opportunities to investigate the occurrence, distribution and transport of radionuclides in the unsaturated and saturated zone at the Nevada Test Site (NTS), Nye County, Nevada. This program is unique becmise of the elevated activities of radionuclides encountered during drilling (> 3.7E+6 Bq/L 3H), extreme completion depths (> 950 m), the expense of constructing new wells (> $IE+6/borehole), and collaboration of government, academic, and industrial partners in the planning and execution of the program. The recent chilling is significant because it substantively augments earlier field of radionuclide migration at NTS, most notably the 1974 CAMBRIC RNM experiment Sites of five nuclear tests fired below or adjacent to the saturated zone have been drilled. Three of the events were fired in Yucca Flat which is a hydrologically closed basin and two were fired in fractured volcanics of Pahute Mesa. Results from Yucca Flat indicate that volatile and refractory radionuclides, fractionated at zero time, we not highly mobile under sawmted conditions. In contrast, borcholes completed on Pahute Mesa indicate Wgh concentrations of tritium (> 3.7E+6 Bq/L 3H) and other radionuclides may be rted more than 300 m from event cavities as dissolved species or as colloids

Recent drilling program to investigate radionuclide migration at the Nevada Test Site

Energy Technology Data Exchange (ETDEWEB)

Smith, D.K.

1997-04-01

Recent drilling affords new opportunities to investigate the occurrence, distribution and transport of radionuclides in the unsaturated and saturated zone at the Nevada Test Site (NTS), Nye County, Nevada. This program is unique becmise of the elevated activities of radionuclides encountered during drilling (> 3.7E+6 Bq/L 3H), extreme completion depths (> 950 m), the expense of constructing new wells (> $IE+6/borehole), and collaboration of government, academic, and industrial partners in the planning and execution of the program. The recent chilling is significant because it substantively augments earlier field of radionuclide migration at NTS, most notably the 1974 CAMBRIC RNM experiment Sites of five nuclear tests fired below or adjacent to the saturated zone have been drilled. Three of the events were fired in Yucca Flat which is a hydrologically closed basin and two were fired in fractured volcanics of Pahute Mesa. Results from Yucca Flat indicate that volatile and refractory radionuclides, fractionated at zero time, we not highly mobile under sawmted conditions. In contrast, borcholes completed on Pahute Mesa indicate Wgh concentrations of tritium (> 3.7E+6 Bq/L 3H) and other radionuclides may be rted more than 300 m from event cavities as dissolved species or as colloids.

Poisoning Experiments Aimed at Discriminating Active and Less-Active Sites of Silica-Supported Tantalum Hydride for Alkane Metathesis

KAUST Repository

Saggio, Guillaume

2010-10-04

Only 50% of the silica-supported tantalum hydride sites are active in the metathesis of propane. Indeed, more than 45% of the tantalum hydride can be eliminated by a selective oxygen poisoning of inactive sites with no significant decrease in the global turnover. Conversely, cyclopentane induces no such selective poisoning. Hence, the active tantalum hydride sites that show greater resistance to oxygen poisoning correspond to the νTa-H bands of higher wavenumbers, particularly that at 1860cm-1. These active tantalum hydride sites should correspond to tris- or monohydride species relatively far from silica surface oxygen atoms. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Status of site investigation for L/ILW facilities in the Philippines - 59262

International Nuclear Information System (INIS)

Palattao, Maria V.; Nohay, Carl; Reyes, Rolando; Singayan, Alfonso; Mallants, Dirk

2012-01-01

Document available in abstract form only. Full text of publication follows: Radioactive waste in the Philippines is generated from the various applications of radioactive materials in medicine, industries and research. The Philippine Nuclear Research Institute (PNRI) currently manages these wastes through its centralized treatment and storage facilities on site. Radioactive wastes that are received at the Institute are of different types ranging from contaminated solid and liquid materials to spent sealed sources, including radium. Treated and conditioned wastes are temporarily stored in simple roofed above ground concrete bunkers. The Philippine government through the Inter-agency Subcommittee on Radioactive Waste Management is committed to the development of a national repository for the country's radioactive waste. This policy has been recognized in spite of the relatively small volume of radioactive waste compared with countries that have a nuclear power program. To date, a potential site has been identified for detailed investigation with the assistance of the International Atomic Energy Agency (IAEA). The site is located in the northern part of the Philippine archipelago and has about 34 hectares for potential development. A drilling program that aimed to investigate the geologic, hydrogeologic and hydrologic properties of the site has been implemented

Supplemental Investigation Plan for FFACO Use Restrictions, Nevada Test Site, Nevada, Revision 0

Energy Technology Data Exchange (ETDEWEB)

Lynn Kidman

2008-02-01

This document is part of an effort to re-evaluate all FFACO URs against the current RBCA criteria (referred to in this document as the Industrial Sites [IS] RBCA process) as defined in the Industrial Sites Project Establishment of Final Action Levels (NNSA/NSO, 2006a). After reviewing all of the existing FFACO URs, the 12 URs addressed in this Supplemental Investigation Plan (SIP) could not be evaluated against the current RBCA criteria as sufficient information about the contamination at each site was not available. This document presents the plan for conducting field investigations to obtain the needed information. This SIP includes URs from Corrective Action Units (CAUs) 326, 339, 358, 452, 454, 464, and 1010, located in Areas 2, 6, 12, 19, 25, and 29 of the Nevada Test Site, which is approximately 65 miles northwest of Las Vegas, Nevada; and CAU 403, located in Area 3 of the Tonopah Test Range, which is approximately 165 miles north of Las Vegas, Nevada.

Geophysical Survey in Sub-Saharan Africa: magnetic and Electromagnetic Investigation of the UNESCO World Heritage Site of Songo Mnara, Tanzania

OpenAIRE

Welham, Kate; Fleisher, J.; Cheetham, Paul; Manley, Harry; Steele, C.; Wynne-Jones, S.

2014-01-01

Magnetometry and Slingram electromagnetic surveys were\\ud conducted at the UNESCO World Heritage Site of Songo Mnara, Tanzania, as part of a multi-national programme of investigation to examine the uses of space within and outside of this stonetown. The town was a major Islamic trading port during the 14th and 15th centuries.The surveys detected significant evidence for the containment of activities within the town walls, and previously unknown anthropogenic activity was revealed between the ...

Concept for calculating dose rates from activated groundwater at accelerator sites

CERN Document Server

Prolingheuer, N; Vanderborght, J; Schlögl, B; Nabbi, R; Moormann, R

Licensing of particle accelerators requires the proof that the groundwater outside of the site will not be significantly contaminated by activation products formed below accelerator and target. In order to reduce the effort for this proof, a site independent simplified but conservative method is under development. The conventional approach for calculation of activation of soil and groundwater is shortly described on example of a site close to Forschungszentrum Juelich, Germany. Additionally an updated overview of a data library for partition coefficients for relevant nuclides transported in the aquifer at the site is presented. The approximate model for transport of nuclides with ground water including exemplary results on nuclide concentrations outside of the site boundary and of resulting effective doses is described. Further applications and developments are finally outlined.

SRP baseline hydrogeologic investigation: Aquifer characterization. Groundwater geochemistry of the Savannah River Site and vicinity

Energy Technology Data Exchange (ETDEWEB)

Strom, R.N.; Kaback, D.S.

1992-03-31

An investigation of the mineralogy and chemistry of the principal hydrogeologic units and the geochemistry of the water in the principal aquifers at Savannah River Site (SRS) was undertaken as part of the Baseline Hydrogeologic Investigation. This investigation was conducted to provide background data for future site studies and reports and to provide a site-wide interpretation of the geology and geochemistry of the Coastal Plain Hydrostratigraphic province. Ground water samples were analyzed for major cations and anions, minor and trace elements, gross alpha and beta, tritium, stable isotopes of hydrogen, oxygen, and carbon, and carbon-14. Sediments from the well borings were analyzed for mineralogy and major and minor elements.

Shemya AFB, Alaska 1992 IRP field investigation report

Energy Technology Data Exchange (ETDEWEB)

1993-02-01

The US Air Force is currently investigating 22 sites on Shemya Air Force Base (AFB) to determine if past spill and disposal activities have caused environmental damage. These investigations are being carried out under the Air Force's Installation Restoration Program (IRP). As a part of the IRP program, field investigations were performed in 1992 to obtain the information needed to assess what future actions willneed to be carried out at each site. The island's drinking water supply was also investigated. Activities completed at 10 selected sites during the 1992 field investigation included surface sampling to determine the lateral extent of contamination, subsurface sampling to determine the vertical extent of contamination, and the installation of well points and monitoring wells to determine the direction of groundwater flow and if the groundwater has been affected by a site. In addition, geophysical surveys were performed at most sites to identify site boundaries and check for the presence of buried metal to be avoided during drilling activities.

Corrective Action Investigation Plan for Corrective Action Unit 550: Smoky Contamination Area Nevada National Security Site, Nevada

International Nuclear Information System (INIS)

Evenson, Grant

2012-01-01

on January 31, 2012, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 550. The potential contamination sources associated with the study groups are from nuclear testing activities conducted at CAU 550. The DQO process resulted in an assumption that the total effective dose (TED) within the default contamination boundary of CAU 550 exceeds the final action level and requires corrective action. The presence and nature of contamination outside the default contamination boundary at CAU 550 will be evaluated based on information collected from a field investigation. Radiological contamination will be evaluated based on a comparison of the TED at sample locations to the dose-based final action level. The TED will be calculated as the total of separate estimates of internal and external dose. Results from the analysis of soil samples will be used to calculate internal radiological dose. Thermoluminescent dosimeters placed at the center of each sample location will be used to measure external radiological dose. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to each group of CASs.

Use of the Multi-Agency Radiological Laboratory Analytical Protocols Manual (MARLAP) for site cleanup activities

International Nuclear Information System (INIS)

Griggs, J.

1999-01-01

MARLAP is being developed as a multi-agency guidance manual for project managers and radioanalytical laboratories. The document uses a performance based approach and will provide guidance and a framework to assure that laboratory radioanalytical data meets the specific project or program needs and requirements. MARLAP supports a wide range of data collection activities including site characterization and compliance demonstration activities. Current participants include: US Environmental Protection Agency (EPA), US Department of Energy (DOE), US Nuclear Regulatory Commission (NRC), US Department of Defense (DoD), US National Institutes of Standards and Technology (NIST), US Geologic Survey (USGS), US Food and Drug Administration (FDA), Commonwealth of Kentucky, and the State of California. MARLAP is the radioanalytical laboratory counterpart to the Multi-Agency Radiological Survey and Site Investigation Manual (MARSSIM). MARLAP is currently in a preliminary draft stage. (author)

Remediation of uranium contaminated sites: clean-up activities in Serbia

International Nuclear Information System (INIS)

Raicevic, S.; Raicevic, J. . E-mail address of corresponding author: raich@beotel.yu; Raicevic, S.)

2005-01-01

One of the serious environmental problems in Serbia represent sites contaminated with depleted uranium (DU) during past war activities. According to UNEP reports and our findings there are two types of contamination: (i) localized points of high, concentrated contamination where DU penetrators enter the soil, and (ii) low level of widespread DU contamination, which indicates that during the conflict DU dust was dispersed into the environment. Remediation of these sites is an urgent need because they represent a permanent threat to the population living in this area. Here we give a brief description of approaches commonly used in remediation of DU contaminated sites, and an overview of current clean-up activities performed in Serbia. (author)

Functional characterization of autophosphorylation sites of the activated insulin receptor-tyrosine kinase

International Nuclear Information System (INIS)

Flores-Riveros, J.R.; Lane, M.D.

1987-01-01

Insulin receptor, solubilized from 3T3-L1 cellular membranes and then purified, was autophosphorylated with [γ- 32 P]ATP in the absence or presence of insulin. Specific phosphopeptides generated by trypsin digestion of the 32 P-labeled β-subunit were identified and separated by reverse phase HPLC. In the absence of insulin, radioactivity of the phosphopeptides is evenly distributed among four major peaks designated as sites I, II, III and IV, according to their order of elution. This pattern is maintained for at least the first 30 min of autophosphorylation. When the reaction is carried out in the presence of insulin, > 50% of the total 32 P radioactivity is found in site I and the rate of 32 P incorporation into this site is markedly higher than into sites II, III and IV. Maximal activation of tyrosine kinase activity, as estimated by substrate phosphorylation, is coincident with the nearly complete phosphorylation of site I. Delayed activation of previously autophosphorylated receptor by insulin, but not by EGF or IGF-I, produced a similar pattern where phosphorylated site I predominates. These observations indicate that one major insulin-regulated autophosphorylation site in the β-subunit is responsible for activation of the insulin receptor tyrosine kinase. The isolation of this phosphopeptide on a preparative scale and its characterization are now in progress

House Church: Investigating Chinese Urban Christians’ Choice of Religious Practicing Site in Wuhan, China

Directory of Open Access Journals (Sweden)

Junqiang Han

2018-03-01

Full Text Available In this paper, we discuss the reasons why urban Christians choose certain sites for religious activities and explain the rapid development of house churches in urban areas in China from the perspective of individual Christians, through survey data obtained by the Chinese Urban Research Center for Ethnic and Religious Affairs Management in the city of Wuhan. We found that Christians who attend religious activities in urban house churches are generally younger in age, higher in education level, and more likely to be working in independent, private, or foreign-invested enterprises. Further investigation reveals that “Three-Self churches” are few in number and poorly planned geographically, resulting in very limited service abilities, thus being far from satisfactory for all believers. Moreover, the activities of such churches are unvaried and lack attractiveness. On the other hand, in house churches, Christians are able to build tight-knit social networks and house churches are more competitively aware in the “religious market”, leading some urban Christians to choose house churches, thereby inciting the fast development of house churches in Chinese urban areas.

Detailed imaging of flowing structures at depth using microseismicity: a tool for site investigation?

Science.gov (United States)

Pytharouli, S.; Lunn, R. J.; Shipton, Z. K.

2011-12-01

Field evidence shows that faults and fractures can act as focused pathways or barriers for fluid migration. This is an important property for modern engineering problems, e.g., CO2 sequestration, geological radioactive waste disposal, geothermal energy exploitation, land reclamation and remediation. For such applications the detailed characterization of the location, orientation and hydraulic properties of existing fractures is necessary. These investigations are expensive, requiring the hire of expensive equipment (excavator or drill rigs), which incur standing charges when not in use. In addition, they only provide information for discrete sample 'windows'. Non-intrusive methods have the ability to gather information across an entire area. Methods including electrical resistivity/conductivity and ground penetrating radar (GRP), have been used as tools for site investigations. Their imaging ability is often restricted due to unfavourable on-site conditions e.g. GRP is not useful in cases where a layer of clay or reinforced concrete is present. Our research has shown that high quality seismic data can be successfully used in the detailed imaging of sub-surface structures at depth; using induced microseismicity data recorded beneath the Açu reservoir in Brazil we identified orientations and values of average permeability of open shear fractures at depths up to 2.5km. Could microseismicity also provide information on the fracture width in terms of stress drops? First results from numerical simulations showed that higher stress drop values correspond to narrower fractures. These results were consistent with geological field observations. This study highlights the great potential of using microseismicity data as a supplementary tool for site investigation. Individual large-scale shear fractures in large rock volumes cannot currently be identified by any other geophysical dataset. The resolution of the method is restricted by the detection threshold of the local

LRRK2 Kinase Activity and Biology are Not Uniformly Predicted by its Autophosphorylation and Cellular Phosphorylation Site Status

Directory of Open Access Journals (Sweden)

April eReynolds

2014-06-01

Full Text Available Missense mutations in the Leucine Rich Repeat protein Kinase 2 (LRRK2 gene are the most common genetic predisposition to develop Parkinson’s disease (PD LRRK2 is a large multi-domain phosphoprotein with a GTPase domain and a serine/threonine protein kinase domain whose activity is implicated in neuronal toxicity; however the precise mechanism is unknown. LRRK2 autophosphorylates on several serine/threonine residues across the enzyme and is found constitutively phosphorylated on Ser910, Ser935, Ser955 and Ser973, which are proposed to be regulated by upstream kinases. Here we investigate the phosphoregulation at these sites by analyzing the effects of disease-associated mutations Arg1441Cys, Arg1441Gly, Ala1442Pro, Tyr1699Cys, Ile2012Thr, Gly2019Ser, and Ile2020Thr. We also studied alanine substitutions of phosphosite serines 910, 935, 955 and 973 and specific LRRK2 inhibition on autophosphorylation of LRRK2 Ser1292, Thr1491, Thr2483 and phosphorylation at the cellular sites. We found that mutants in the Roc-COR domains, including Arg1441Cys, Arg1441His, Ala1442Pro and Tyr1699Cys, can positively enhance LRRK2 kinase activity while concomitantly inducing the dephosphorylation of the cellular sites. Mutation of the cellular sites individually did not affect LRRK2 intrinsic kinase activity; however, Ser910/935/955/973Ala mutations trended toward increased kinase activity of LRRK2. Increased cAMP levels did not lead to increased LRRK2 cellular site phosphorylation, 14-3-3 binding or kinase activity. In cells, inhibition of LRRK2 kinase activity leads to dephosphorylation of Ser1292 by Calyculin A and okadaic acid sensitive phosphatases, while the cellular sites are dephosphorylated by Calyculin A sensitive phosphatases. These findings indicate that comparative analysis of both Ser1292 and Ser910/935/955/973 phosphorylation sites will provide important and distinct measures of LRRK2 kinase and biological activity in vitro and in vivo.

Phase I Source Investigation, Heckathorn Superfund Site, Richmond, California

Energy Technology Data Exchange (ETDEWEB)

Kohn, Nancy P; Evans, Nathan R

2002-12-18

This report represents Phase I of a multi-phase approach to a source investigation of DDT at the Heckathorn Superfund Site, Richmond, California, the former site of a pesticide packaging plant, and the adjacent waterway, the Lauritzen Channel. Potential identified sources of contamination were from sloughed material from undredged areas (such as side banks) and from outfall pipes. Objectives of Phase I included the (1) evaluation of pesticide concentrations associated with discharge from outfalls, (2) identification of additional outfalls in the area, (3) identification of type, quantity, and distribution of sediment under the Levin pier, (4) quantification of pesticide concentrations in sediment under the pier, and (5) evaluation of sediment structure and slope stability under the pier. Field operations included the collection of sediment directly from inside the mouths of outfall pipes, when possible, or the deployment of specially designed particle traps where direct sampling was problematic. Passive water samplers were placed at the end of known outfall pipes and analyzed for DDT and other pesticides of concern. Underwater dive surveys were conducted beneath the Levin pier to document type, slope, and thickness of sediment. Samples were collected at locations of interest and analyzed for contaminants. Also sampled was soil from bank areas, which were suspected of potentially contributing to continued DDT contamination of the Lauritzen Channel through erosion and groundwater leaching. The Phase I Source Investigation was successful in identifying significant sources of DDT contamination to Lauritzen Channel sediment. Undredged sediment beneath the Levin pier that has been redistributed to the channel is a likely source. Two outfalls tested bear further investigation. Not as well-defined are the contributions of bank erosional material and groundwater leaching. Subsequent investigations will be based on the results of this first phase.

Cavola experiment site: geophysical investigations and deployment of a dense seismic array on a landslide

Directory of Open Access Journals (Sweden)

L. Martelli

2007-06-01

Full Text Available Geophysical site investigations have been performed in association with deployment of a dense array of 95 3-component seismometers on the Cavola landslide in the Northern Apennines. The aim of the array is to study propagation of seismic waves in the heterogeneous medium through comparison of observation and modelling. The small-aperture array (130 m×56 m operated continuously for three months in 2004. Cavola landslide consists of a clay body sliding over mudstone-shale basement, and has a record of historical activity, including destruction of a small village in 1960. The site investigations include down-hole logging of P- and S-wave travel times at a new borehole drilled within the array, two seismic refraction lines with both P-wave profiling and surface-wave analyses, geo-electrical profiles and seismic noise measurements. From the different approaches a consistent picture of the depths and seismic velocities for the landslide has emerged. Their estimates agree with resonance frequencies of seismic noise, and also with the logged depths to basement of 25 m at a new borehole and of 44 m at a pre-existing borehole. Velocities for S waves increase with depth, from 230 m/s at the surface to 625 m/s in basement immediately below the landslide.

Active Sites Environmental Monitoring Program. FY 1993: Annual report

International Nuclear Information System (INIS)

Morrissey, C.M.; Ashwood, T.L.; Hicks, D.S.; Marsh, J.D.

1994-08-01

This report continues a series of annual and semiannual reports that present the results of the Active Sites Environmental Monitoring Program (ASEMP) monitoring activities. The report details monitoring data for fiscal year (FY) 1993 and is divided into three major areas: SWSA 6 [including tumulus pads, Interim Waste Management Facility (IWMF), and other sites], the low-level Liquid-Waste Solidification Project (LWSP), and TRU-waste storage facilities in SWSA 5 N. The detailed monitoring methodology is described in the second revision of the ASEMP program plan. This report also presents a summary of the methodology used to gather data for each major area along with the results obtained during FY 1993

Savannah River Site RCRA Facility Investigation plan: Road A Chemical Basin

International Nuclear Information System (INIS)

1989-06-01

The nature of wastes disposed of at the Road A Chemical Basin (RACB) is such that some degree of soil contamination is probable. Lead has also been detected in site monitoring wells at concentrations above SRS background levels. A RCRA Facility Investigation (RFI) is proposed for the RACB and will include a ground penetrating radar (GPR) survey, collection and chemical and radiological analyses of soil cores, installation of groundwater monitoring wells, collection and chemical and radiological analyses of groundwater samples, and collection of chemical and radiological analyses of surface water and sediment samples. Upon completion of the proposed RFI field work and chemical and radiological analyses, and RFI report should be prepared to present conclusions on the nature and extent of contamination at the site, and to make recommendations for site remediation. If contamination is detected at concentrations above SRS background levels, a receptor analysis should be done to evaluate potential impacts of site contamination on nearby populations

Essential histidyl residues at the active site(s) of sucrose-phosphate synthase from Prosopis juliflora.

Science.gov (United States)

Sinha, A K; Pathre, U V; Sane, P V

1998-11-10

Chemical modification of sucrose-phosphate synthase (EC 2.4.1.14) from Prosopis juliflora by diethyl pyrocarbonate (DEP) and photo-oxidation in the presence of rose bengal (RB) which modify the histidyl residues of the protein resulted in the inactivation of the enzyme activity. This inactivation was dependent on the concentration of the modifying reagent and the time of incubation and followed pseudo-first order kinetics. For both the reagents, the inactivation was maximum at pH 7.5, which is consistent with the involvement and presence of histidine residues at the active site of the enzyme. Substrates, UDPG and F6P protected the enzyme against the inactivation by the modifying reagents suggesting that the histidine residues may be involved in the binding of these substrates and are essential for the catalytic activity. Specificity of DEP was indicated by an increase in absorbance at 240 nm along with concomitant inactivation of the enzyme and reactivation of the modified enzyme by hydroxylamine. These results strongly suggest the presence of histidine residue(s) at or near the active site of the enzyme.

FInal Report: Site Investigation Results, 2009-2011, at Inman, Kansas

Energy Technology Data Exchange (ETDEWEB)

LaFreniere, Lorraine M. [Argonne National Lab. (ANL), Argonne, IL (United States). Environmental Sciences Div.

2015-05-01

The Commodity Credit Corporation of the U.S. Department of Agriculture (CCC/USDA) operated a grain storage facility at the southern edge of the city of Inman, Kansas, from 1954 to 1965. During this time, commercial grain fumigants containing carbon tetrachloride were in common use by the grain storage industry to preserve grain in their facilities. In 1997, trace to low levels of carbon tetrachloride (below the maximum contamination level [MCL] of 5.0 μg/L) were detected in three private wells near the former grain storage facility at Inman, as part of a statewide USDA private well sampling program that was implemented by the Kansas Department of Health and Environment (KDHE) near former CCC/USDA facilities. No public water supply wells were identified in 1998 by the KDHE within 1 mi of the town. Carbon tetrachloride is the contaminant of primary concern at sites associated with grain storage operations. To determine whether the former CCC/USDA facility at Inman is a potential contaminant source and its possible relationship to the contamination in groundwater, the CCC/USDA agreed to conduct investigations at Inman. The investigations were performed by the Environmental Science Division of Argonne National Laboratory in accordance with the Intergovernmental Agreement between the KDHE and the Farm Service Agency of the USDA. Argonne, on behalf of the CCC/USDA, developed a Work Plan (Argonne 2007) and subsequently a Summary of Investigation Results and Proposed Work Plan (Appendix A) for a phased site investigation. The proposed work was approved by the KDHE (2007, 2011). The investigations were conducted from November 2009 to September 2011, as proposed in the two work plans. This report presents the findings of the 2009-2011 investigations at Inman.

Learning of the subject: Methodology of investigation by means of a Site Web.

Directory of Open Access Journals (Sweden)

Yolanda Margarita Carbonell Cabarga

2010-10-01

Full Text Available Results of an investigation are presented having as an objective: the design of a Web Site for the learning of Methodology of Investigation subject in Psychology Bachelor of the Municipal University of Sancti Spíritus. This study was carried out in students of psychology career who were facing first year. Methods of the theoretical and empiric level were applied as well as the Mathematical Statistic. In the diagnosis was possible to appreciate the ignorance regarding the use of the methodology in the therorical methodological design of an investigation, also the difficulties in the study of the subject and violations in the application of orientations from the professors at the time of doing the research papers, so an interactive Web Site was obtained and applied which allow the teaching of the subject. The site was validated by means of a quasi-experiment, the obtained information was processed with the use of the statistical inferentia, results were in general highly significant for the feasibility of the proposal.

Rac1 GTPase activates the WAVE regulatory complex through two distinct binding sites

Science.gov (United States)

Brautigam, Chad A; Xing, Wenmin; Yang, Sheng; Henry, Lisa; Doolittle, Lynda K; Walz, Thomas

2017-01-01

The Rho GTPase Rac1 activates the WAVE regulatory complex (WRC) to drive Arp2/3 complex-mediated actin polymerization, which underpins diverse cellular processes. Here we report the structure of a WRC-Rac1 complex determined by cryo-electron microscopy. Surprisingly, Rac1 is not located at the binding site on the Sra1 subunit of the WRC previously identified by mutagenesis and biochemical data. Rather, it binds to a distinct, conserved site on the opposite end of Sra1. Biophysical and biochemical data on WRC mutants confirm that Rac1 binds to both sites, with the newly identified site having higher affinity and both sites required for WRC activation. Our data reveal that the WRC is activated by simultaneous engagement of two Rac1 molecules, suggesting a mechanism by which cells may sense the density of active Rac1 at membranes to precisely control actin assembly. PMID:28949297

Nuclear waste: Status of DOE's nuclear waste site characterization activities

International Nuclear Information System (INIS)

1987-01-01

Three potential nuclear waste repository sites have been selected to carry out characterization activities-the detailed geological testing to determine the suitability of each site as a repository. The sites are Hanford in south-central Washington State, Yucca Mountain in southern Nevada, and Deaf Smith in the Texas Panhandle. Two key issues affecting the total program are the estimations of the site characterization completion data and costs and DOE's relationship with the Nuclear Regulatory Commission which has been limited and its relations with affected states and Indian tribes which continue to be difficult

Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis

NARCIS (Netherlands)

Bokma, Evert; Rozeboom, Henriëtte J.; Sibbald, Mark; Dijkstra, Bauke W.; Beintema, Jaap J.

Hevamine is a chitinase from the rubber tree Hevea brasiliensis. Its active site contains Asp125, Glu127, and Tyr183, which interact with the -1 sugar residue of the substrate. To investigate their role in catalysis, we have successfully expressed wild-type enzyme and mutants of these residues as

Preliminary safety evaluation for the Simpevarp subarea. Based on data and site descriptions after the initial site investigation stage

International Nuclear Information System (INIS)

2005-04-01

The main objectives of this Preliminary safety evaluation (PSE) of the Simpevarp subarea are: to determine, whether the feasibility study's judgement of the suitability of the candidate area with respect to long-term safety holds up in the light of the site investigation data; to provide feedback to continued site investigations and site-specific repository design and to identify site specific scenarios and geoscientific issues for further analyses. The PSE focuses on comparing the attained knowledge of the sites with the suitability criteria as set out by SKB in the report SKB-TR--00-12. These criteria both concern properties of the site judged to be necessary for safety and engineering (requirements) and properties judged to be beneficial (preferences). The findings are then evaluated in order to provide feedback to continued investigations and design work. The PSE does not aim at comparing sites and does not assess compliance with safety and radiation protection criteria. The evaluation shows that even considering remaining uncertainties, the Simpevarp subarea meets all safety requirements and most of the safety preferences. Consequently, from a safety point of view, there is no reason not to continue the Site Investigations of the Simpevarp subarea. There are still uncertainties to resolve and the safety would eventually need to be verified through a full safety assessment. Still, this Preliminary Safety Evaluation demonstrates that it is likely that a safe repository for spent nuclear fuel of the KBS-3 type could be constructed at the site. The following feedback is provided to the site investigations and the associated site modelling: Reducing the uncertainty on the deformation zone geometry within the Simpevarp subarea would allow for a more specified layout, although the sensitivity analysis shows that the space needed is rather robust with respect to uncertainties in the zones. There is substantial uncertainty in the discrete fracture network (DFN) model

Single-Site Palladium(II) Catalyst for Oxidative Heck Reaction: Catalytic Performance and Kinetic Investigations

Energy Technology Data Exchange (ETDEWEB)

Duan, Hui; Li, Mengyang; Zhang, Guanghui; Gallagher, James R.; Huang, Zhiliang; Sun, Yu; Luo, Zhong; Chen, Hongzhong; Miller, Jeffrey T.; Zou, Ruqiang; Lei, Aiwen; Zhao, Yanli

2015-01-01

ABSTRACT: The development of organometallic single-site catalysts (SSCs) has inspired the designs of new heterogeneous catalysts with high efficiency. Nevertheless, the application of SSCs in certain modern organic reactions, such as C-C bond formation reactions, has still been less investigated. In this study, a single-site Pd(II) catalyst was developed, where 2,2'-bipyridine-grafted periodic mesoporous organosilica (PMO) was employed as the support of a Pd(II) complex. The overall performance of the single-site Pd(II) catalyst in the oxidative Heck reaction was then investigated. The investigation results show that the catalyst displays over 99% selectivity for the product formation with high reaction yield. Kinetic profiles further confirm its high catalytic efficiency, showing that the rate constant is nearly 40 times higher than that for the free Pd(II) salt. X-ray absorption spectroscopy reveals that the catalyst has remarkable lifetime and recyclability.

What Motivates Young Adults to Talk About Physical Activity on Social Network Sites?

Science.gov (United States)

Zhang, Ni; Campo, Shelly; Yang, Jingzhen; Eckler, Petya; Snetselaar, Linda; Janz, Kathleen; Leary, Emily

2017-06-22

Electronic word-of-mouth on social network sites has been used successfully in marketing. In social marketing, electronic word-of-mouth about products as health behaviors has the potential to be more effective and reach more young adults than health education through traditional mass media. However, little is known about what motivates people to actively initiate electronic word-of-mouth about health behaviors on their personal pages or profiles on social network sites, thus potentially reaching all their contacts on those sites. This study filled the gap by applying a marketing theoretical model to explore the factors associated with electronic word-of-mouth on social network sites about leisure-time physical activity. A Web survey link was sent to undergraduate students at one of the Midwestern universities and 439 of them completed the survey. The average age of the 439 participants was 19 years (SD=1 year, range: 18-24). Results suggested that emotional engagement with leisure-time physical activity (ie, affective involvement in leisure-time physical activity) predicted providing relevant opinions or information on social network sites. Social network site users who perceived stronger ties with all their contacts were more likely to provide and seek leisure-time physical activity opinions and information. People who provided leisure-time physical activity opinions and information were more likely to seek opinions and information, and people who forwarded information about leisure-time physical activity were more likely to chat about it. This study shed light on the application of the electronic word-of-mouth theoretical framework in promoting health behaviors. The findings can also guide the development of future social marketing interventions using social network sites to promote leisure-time physical activity. ©Ni Zhang, Shelly Campo, Jingzhen Yang, Petya Eckler, Linda Snetselaar, Kathleen Janz, Emily Leary. Originally published in the Journal of Medical

Hanfor site past practice investigation strategy

International Nuclear Information System (INIS)

Thompson, K.M.

1991-01-01

The US Department of Energy (DOE), US Environmental Protection Agency (EPA), and the Washington State Department of Ecology (Ecology) have negotiated a strategy for performing Comprehensive Environmental Response, Compensation and Liability Act (CERCLA) and Resource Conservation and Recovery Act (RCRA) Past Practice investigations in a more streamlined manner with a bias-for-action. This strategy provides new concepts for (1) accelerating decision-making by maximizing the use of existing data consistent with data quality objectives and (2) undertaking expedited response actions and/or interim remedial measures as appropriate to either remove threats to human health and welfare and the environment or to reduce risk by reducing toxicity, mobility or volume of contaminants. Since the goal of the program is cleanup, much more emphasis will be placed on initiating and completing waste site cleanups through interim measures. While investigations and studies are important in meeting long-range goals, there is now agreement by the parties that an appropriate and significant portion of the near-term funding resources can and should be dedicated to remedial work, where there is sufficient information from which to plan and implement interim remedial measures. The initial stages of Hanford clean-up will optimize the use of interim cleanup actions when justified and practicable. Existing data will be evaluated as the initial basis for decision-making. If the data are found to be insufficient, additional essential data will be collected to support the IRM in a limited field investigation (LFI). Only data needed to formulate a conceptual model (source to pathway to receptor) and qualitative risk assessment would be obtained. The data quality objectives of the LFI will be established based on the use of the data in deciding on IRMs

Transport processes investigation: A necessary first step in site scale characterization plans

International Nuclear Information System (INIS)

Roepke, C.; Glass, R.J.; Brainard, J.; Mann, M.; Kriel, K.; Holt, R.; Schwing, J.

1995-01-01

We propose an approach, which we call the Transport Processes Investigation or TPI, to identify and verify site-scale transport processes and their controls. The TPI aids in the formulation of an accurate conceptual model of flow and transport, an essential first step in the development of a cost effective site characterization strategy. The TPI is demonstrated in the highly complex vadose zone of glacial tills that underlie the Fernald Environmental Remediation Project (FEMP) in Fernald, Ohio. As a result of the TPI, we identify and verify the pertinent flow processes and their controls, such as extensive macropore and fracture flow through layered clays, which must be included in an accurate conceptual model of site-scale contaminant transport. We are able to conclude that the classical modeling and sampling methods employed in some site characterization programs will be insufficient to characterize contaminant concentrations or distributions at contaminated or hazardous waste facilities sited in such media

Structuring a cost-effective site characterization

International Nuclear Information System (INIS)

Berven, B.A.; Little, C.A.; Swaja, R.E.

1990-01-01

Successful chemical and radiological site characterizations are complex activities which require meticulously detailed planning. Each layer of investigation is based upon previously generated information about the site. Baseline historical, physical, geological, and regulatory information is prerequisite for preliminary studies at a site. Preliminary studies then provide samples and measurements which define the identity of potential contaminants and define boundaries around the area to be investigated. The goal of a full site characterization is to accurately determine the extent and magnitude of contaminants and carefully define the site conditions such that the future movements of site contaminants can be assessed for potential exposure to human occupants and/or environmental impacts. Critical to this process is the selection of appropriate measurement and sampling methodology, selection and use of appropriate instrumentation and management/interpretation of site information. Site investigations require optimization between the need of information to maximize the understanding of site conditions and the cost of acquiring that information. 5 refs., 1 tab

Corrective Action Investigation Plan for Corrective Action Unit 569: Area 3 Yucca Flat Atmospheric Test Sites Nevada National Security Site, Nevada, Revision 0

Energy Technology Data Exchange (ETDEWEB)

Patrick Matthews; Christy Sloop

2012-02-01

Corrective Action Unit (CAU) 569 is located in Area 3 of the Nevada National Security Site, which is approximately 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 569 comprises the nine numbered corrective action sites (CASs) and one newly identified site listed below: (1) 03-23-09, T-3 Contamination Area (hereafter referred to as Annie, Franklin, George, and Moth); (2) 03-23-10, T-3A Contamination Area (hereafter referred to as Harry and Hornet); (3) 03-23-11, T-3B Contamination Area (hereafter referred to as Fizeau); (4) 03-23-12, T-3S Contamination Area (hereafter referred to as Rio Arriba); (5) 03-23-13, T-3T Contamination Area (hereafter referred to as Catron); (6) 03-23-14, T-3V Contamination Area (hereafter referred to as Humboldt); (7) 03-23-15, S-3G Contamination Area (hereafter referred to as Coulomb-B); (8) 03-23-16, S-3H Contamination Area (hereafter referred to as Coulomb-A); (9) 03-23-21, Pike Contamination Area (hereafter referred to as Pike); and (10) Waste Consolidation Site 3A. Because CAU 569 is a complicated site containing many types of releases, it was agreed during the data quality objectives (DQO) process that these sites will be grouped. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each study group. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the DQOs developed on September 26, 2011, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office. The DQO

Cooperative activation of cardiac transcription through myocardin bridging of paired MEF2 sites

Energy Technology Data Exchange (ETDEWEB)

Anderson, Courtney M. [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Hu, Jianxin [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Thomas, Reuben [Univ. of California, San Francisco, CA (United States). Gladstone Inst.; Gainous, T. Blair [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Celona, Barbara [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Sinha, Tanvi [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Dickel, Diane E. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Genomics Division; Heidt, Analeah B. [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Xu, Shan-Mei [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Bruneau, Benoit G. [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Univ. of California, San Francisco, CA (United States). Gladstone Inst.; Pollard, Katherine S. [Univ. of California, San Francisco, CA (United States). Gladstone Inst.; Pennacchio, Len A. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Genomics Division; Black, Brian L. [Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.; Univ. of California, San Francisco, CA (United States). Dept. of

2017-03-28

Enhancers frequently contain multiple binding sites for the same transcription factor. These homotypic binding sites often exhibit synergy, whereby the transcriptional output from two or more binding sites is greater than the sum of the contributions of the individual binding sites alone. Although this phenomenon is frequently observed, the mechanistic basis for homotypic binding site synergy is poorly understood. Here in this paper, we identify a bona fide cardiac-specific Prkaa2 enhancer that is synergistically activated by homotypic MEF2 binding sites. We show that two MEF2 sites in the enhancer function cooperatively due to bridging of the MEF2C-bound sites by the SAP domain-containing co-activator protein myocardin, and we show that paired sites buffer the enhancer from integration site-dependent effects on transcription in vivo. Paired MEF2 sites are prevalent in cardiac enhancers, suggesting that this might be a common mechanism underlying synergy in the control of cardiac gene expression in vivo.

The value of DCIP geophysical surveys for contaminated site investigations

DEFF Research Database (Denmark)

Balbarini, Nicola; Rønde, Vinni Kampman; Maurya, Pradip Kumar

an old factory site by combining traditional geological, hydrological, and contaminant concentration data with DCIP surveys. The plume consisted of xenobiotic organic compounds and inorganics. The study assesses benefits and limitations of DCIP geophysics for contaminated site investigations. A 3D......Geophysical methods are increasingly being used in contaminant hydrogeology to map lithology, hydraulic properties, and contaminant plumes with a high ionic strength. Advances in the Direct Current resistivity and Induced Polarization (DCIP) method allow the collection of high resolution three...... water and below the streambed. Surface DCIP surveys supported the characterization of the spatial variability in geology, hydraulic conductivity and contaminant concentration. Though DCIP data interpretation required additional borehole data, the DCIP survey reduced the number of boreholes required...

Study of site layout in the Rokkasho site

International Nuclear Information System (INIS)

Sato, Kazuyoshi; Tamura, Kousaku; Yagenji, Akira; Sekiya, Shigeki; Takahashi, Hideo; Neyatani, Yuzuru; Uehara, Masaharu; Motohashi, Keiichi; Hashimoto, Masayoshi; Ogino, Shunji; Nagamatsu, Nobuhide

2006-03-01

The Final Design Report (FDR) of the International Thermonuclear Experimental Reactor (ITER) was published on July 2001 as a summary of the Engineering Design Activity (EDA). After the EDA, site dependent design has been investigated for the invitation of ITER toward Rokkasho Site (Iyasakadai area) in Aomori prefecture. This report describes the results of site layout of major buildings and structures of ITER in the Rokkasho-Site. The data of the ground near the site and the results of site dependent design in Japan were applied to this study. Through this study, the most appropriate site layout has been constructed with satisfaction of following conditions. (1) Bedrock level at the tokamak complex building is relatively high and it can be reduced the cost of excavation and foundation work. (2) Total amount of excavation soil for site preparation is minimized and the flexibility of the layout is ensured with flat ground level. (3) Accessibility of human and equipments, reduction of noise and vibration to the environment can be obtained. Total length of ducts and piping between buildings in site is minimized. (author)

Geochemical investigation of UMTRAP designated site at Canonsburg, Pennsylvania

International Nuclear Information System (INIS)

Markos, G.; Bush, K.J.; Freeman, T.

1981-05-01

UMTRAP is an acronym for Uranium Mill Tailings Remedial Action Project. The Canonsburg, Pennsylvania site was utilized from 1911 to 1922 and from 1930 to 1942, first to remove radium from sedimentary uranium ore, and later to remove uranium from ore as well as process various materials, ceasing operations in 1957. Of the three areas, Area A is the former mill site and is occupied in part by present industrial activities. Area B is comprised of mill residues mixed with sediment dredged from Chartiers Creek, a disturbed terrace along Chartiers Creek, and a ditch on the north side. The ditch contains water with the highest concentrations of contaminants measured in the Canonsburg site. It is believed that unprocessed ore is deposited here and effluent from leaks in a sewer line across the north edge of Areas A and B carry contaminants from heavily contaminated drains in the buildings of Area A. Area C contains the former liquid disposal lagoon, and the central part of the area is highly contaminated to a depth of about 3 meters. Area C contains the most concentrated waste material with maximum levels (except for the ditch on the north side of Area B) of all the contaminants distributed throughout the area. Contamination outside the site is limited. The presence of contaminants in the ditches, in the soils on the river bank, and below the tailings pile is not attributed to migration processes, but rather to the presence of ore materials and concentrated wastes mechanically dispersed throughout the area

Modelling of water-rock interaction at TVO investigation sites

International Nuclear Information System (INIS)

Pitkaenen, P.; Leino-Forsman, H.

1992-12-01

The geochemistry of the groundwater at the Kivetty, Syyry and Olkiluoto site investigation areas in Finland for nuclear waste disposal is evaluated. The hydrogeological data is collected from boreholes drilled down to 100-m depth into crystalline bedrock. The interpretation is based on groundwater chemistry and isotope data, mineralogical data, and the structure and hydrology of the bedrock, using correlation diagrams and the thermodynamic calculations (PHREEQE,EQ3NR). The hydrogeochemistry and major processes controlling the groundwater chemistry are discussed

Immobilized low-activity waste site borehole 299-E17-21

International Nuclear Information System (INIS)

Reidel, S.P.; Reynolds, K.D.; Horton, D.G.

1998-08-01

The Tank Waste Remediation System (TWRS) is the group at the Hanford Site responsible for the safe underground storage of liquid waste from previous Hanford Site operations, the storage and disposal of immobilized tank waste, and closure of underground tanks. The current plan is to dispose of immobilized low-activity tank waste (ILAW) in new facilities in the southcentral part of 200-East Area and in four existing vaults along the east side of 200-East Area. Boreholes 299-E17-21, B8501, and B8502 were drilled at the southwest corner of the ILAW site in support of the Performance Assessment activities for the disposal options. This report summarizes the initial geologic findings, field tests conducted on those boreholes, and ongoing studies. One deep (480 feet) borehole and two shallow (50 feet) boreholes were drilled at the southwest corner of the ILAW site. The primary factor dictating the location of the boreholes was their characterization function with respect to developing the geohydrologic model for the site and satisfying associated Data Quality Objectives. The deep borehole was drilled to characterize subsurface conditions beneath the ILAW site, and two shallow boreholes were drilled to support an ongoing environmental tracer study. The tracer study will supply information to the Performance Assessment. All the boreholes provide data on the vadose zone and saturated zone in a previously uncharacterized area

Orthogonal use of a human tRNA synthetase active site to achieve multifunctionality.

Science.gov (United States)

Zhou, Quansheng; Kapoor, Mili; Guo, Min; Belani, Rajesh; Xu, Xiaoling; Kiosses, William B; Hanan, Melanie; Park, Chulho; Armour, Eva; Do, Minh-Ha; Nangle, Leslie A; Schimmel, Paul; Yang, Xiang-Lei

2010-01-01

Protein multifunctionality is an emerging explanation for the complexity of higher organisms. In this regard, aminoacyl tRNA synthetases catalyze amino acid activation for protein synthesis, but some also act in pathways for inflammation, angiogenesis and apoptosis. It is unclear how these multiple functions evolved and how they relate to the active site. Here structural modeling analysis, mutagenesis and cell-based functional studies show that the potent angiostatic, natural fragment of human tryptophanyl-tRNA synthetase (TrpRS) associates via tryptophan side chains that protrude from its cognate cellular receptor vascular endothelial cadherin (VE-cadherin). VE-cadherin's tryptophan side chains fit into the tryptophan-specific active site of the synthetase. Thus, specific side chains of the receptor mimic amino acid substrates and expand the functionality of the active site of the synthetase. We propose that orthogonal use of the same active site may be a general way to develop multifunctionality of human tRNA synthetases and other proteins.

Animal investigation program 1978 annual report: Nevada Test Site and vicinity

International Nuclear Information System (INIS)

Smith, D.D.; Bernhardt, D.E.; Giles, K.R.

1980-12-01

Data are presented from the radioanalyses of tissues collected from cattle, mule deer, desert bighorn sheep, rabbits, golden eagles, and other wildlife that resided on or near the Nevada Test Site during 1978. Routine and special activities of the Animal Investigation Program are also discussed. Other than the naturally occurring Potassium-40, gamma-emitting radionuclides were detected infrequently with the exception of short-lived radionuclides found in samples from animals collected soon after March 14 (the date of a nuclear test by the People's Republic of China). Strontium-90 concentrations in bones from deer, cattle, and desert bighorn sheep were consistent with those of recent years. Tritium concentrations were generally within expected environmental limits with the exception of animals exposed to sources of contamination; e.g., drainage ponds from Area 12 tunnels. Plutonium levels in all tissues from all species showed little variation from recent years. However, cattle tissue sampled in the fall were higher than those collected in the spring. Radionuclide tissue concentrations were generally higher in the tissues of animals residing in Area 15 than in similar animals collected from other Nevada Test Site areas. Hypothetical dose estimates to man were calculated on the basis of the daily consumption of 0.5 kilogram of liver or muscle from animals that contained peak radionuclide levels. The movements of 13 mule deer outfitted with collars containing a radio transmitter unit were monitored on a weekly basis. No gross or microscopic lesions were found in necropsied animals that could be directly attributed to the effects of ionizing radiation

Animal investigation program 1980 annual report: Nevada Test Site and vicinity

International Nuclear Information System (INIS)

Smith, D.D.; Giles, K.R.; Bernhardt, D.E.

1982-08-01

Data are presented from the radioanalyses of tissues collected from cattle, mule deer, desert bighorn sheep, rabbits, and a horse that resided on or near the Nevada Test Site during 1980. Routine and special activities of the Animal Investigation Program are also discussed. Other than the naturally occurring 40 K, gamma-emitting radionuclides were detected infrequently. 131 I was found in the thyroid of a deer 3 weeks after a nuclear test by the People's Republic of China. Concentrations of 90 Sr in bones from deer, cattle, and desert bighorn sheep were similar to those of recent years. Plutonium levels in all tissues from all species showed little variation from those levels in samples collected in recent years. Radionuclide concentrations were generally higher in the tissues of animals residing in Area 15 than in similar animals collected from other Nevada Test Site areas. Surface soil samples from the Area 15 farm contained 238 Pu and 239 Pu in nanocurie per kilogram concentrations. Hypothetical annual dose estimates to man were calculated on the basis of the daily consumption of 0.5 kilogram of liver or muscle from animals that contained peak radionuclide levels. The highest postulated dose was 0.4 millirems to whole body for 137 Cs in muscle obtained from cattle. This dose is about 0.1 percent of the 500 millirems per year radiation protection guide for individuals in the general population. All other postulated doses for consumption of tissues containing other radionuclides were less than 0.1 percent of the standard

In silico analysis of Pycnoporus cinnabarinus laccase active site with toxic industrial dyes.

Science.gov (United States)

Prasad, Nirmal K; Vindal, Vaibhav; Narayana, Siva Lakshmi; Ramakrishna, V; Kunal, Swaraj Priyaranjan; Srinivas, M

2012-05-01

Laccases belong to multicopper oxidases, a widespread class of enzymes implicated in many oxidative functions in various industrial oxidative processes like production of fine chemicals to bioremediation of contaminated soil and water. In order to understand the mechanisms of substrate binding and interaction between substrates and Pycnoporus cinnabarinus laccase, a homology model was generated. The resulted model was further validated and used for docking studies with toxic industrial dyes- acid blue 74, reactive black 5 and reactive blue 19. Interactions of chemical mediators with the laccase was also examined. The docking analysis showed that the active site always cannot accommodate the dye molecules, due to constricted nature of the active site pocket and steric hindrance of the residues whereas mediators are relatively small and can easily be accommodated into the active site pocket, which, thereafter leads to the productive binding. The binding properties of these compounds along with identification of critical active site residues can be used for further site-directed mutagenesis experiments in order to identify their role in activity and substrate specificity, ultimately leading to improved mutants for degradation of these toxic compounds.

Corrective Action Investigation Plan for Corrective Action Unit 166: Storage Yards and Contaminated Materials, Nevada Test Site, Nevada

International Nuclear Information System (INIS)

David Strand

2006-01-01

Corrective Action Unit 166 is located in Areas 2, 3, 5, and 18 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit (CAU) 166 is comprised of the seven Corrective Action Sites (CASs) listed below: (1) 02-42-01, Cond. Release Storage Yd - North; (2) 02-42-02, Cond. Release Storage Yd - South; (3) 02-99-10, D-38 Storage Area; (4) 03-42-01, Conditional Release Storage Yard; (5) 05-19-02, Contaminated Soil and Drum; (6) 18-01-01, Aboveground Storage Tank; and (7) 18-99-03, Wax Piles/Oil Stain. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation (CAI) before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on February 28, 2006, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and Bechtel Nevada. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 166. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to each CAS. The scope of the CAI for CAU 166 includes the following activities: (1) Move surface debris and/or materials, as needed, to facilitate sampling. (2) Conduct radiological surveys. (3) Perform field screening. (4) Collect and submit environmental samples for laboratory analysis to determine if

Dry-run of site investigation planning using the manual for preliminary investigation in Japan

International Nuclear Information System (INIS)

Akamura, Shigeki; Miwa, Tadashi; Tanaka, Tatsuya; Shiratsuchi, Hiroshi; Horio, Atsushi

2011-01-01

A stepwise site selection process has been adopted for geological disposal of HLW in Japan. Literature surveys, followed by preliminary investigations (PI) and, finally, detailed investigations in underground facilities will be carried out in the successive selection stages. In the PI stage, surface-based investigations such as borehole surveys and geophysical prospecting will be implemented. In order to conduct the PI appropriately and efficiently within a restricted timeframe and budget, planning and management of PI are very important. NUMO therefore compiled existing knowledge and experience in the planning and managing of investigations in the form of manuals to be used to improve and maintain internal expertise. The first editions of the two manuals were prepared on the basis of experience overseas, and then they were revised by taking technological environment, laws and regulation in Japan into consideration. This paper introduces the procedure of PI planning using manual as well as the results of the dry-run, with the Yokosuka area as a hypothetical PI area, where the monstraction study is under way. Based on the dry-run, applicability of the manual is checked and, at the same time, further revisions are made to improve the content. (author)

Integrated site investigation and groundwater monitoring in an urban environment

Science.gov (United States)

Weatherl, R. K.

2017-12-01

Understanding groundwater dynamics around cities and other areas of human influence is of crucial importance for water resource management and protection, especially in a time of environmental and societal change. The human environment presents a unique challenge in terms of hydrological characterization, as the water cycle is generally artificialized and emissions of treated waste and chemical products into the surface- and groundwater system tend to disrupt the natural aqueous signature in significant ways. This project presents an integrated approach for robust characterization and monitoring of an urban aquifer which is actively exploited for municipal water supply. The study is carried out in the town of Fehraltorf, in the canton of Zürich, Switzerland. This particular town encompasses industrial and agricultural zones in addition to its standard urban setting. A minimal amount of data exist at this site, and the data that do exist are spatially and temporally sparse. Making use of traditional hydrogeological methods alongside evolving and emerging technologies, we aim to identify sources of contamination and to define groundwater flow and solute transport through space and time. Chemical and physical indicator parameters are identified for tracing contaminations including micropollutants and plant nutrients. Wireless sensors are installed for continuous on-line monitoring of essential parameters (electrical conductivity, temperature, water level). A wireless sensor network has previously been installed in the sewer system of the study site, facilitating investigation into interactions between sewer water and groundwater. Our approach illustrates the relations between land use, climate, rainfall dynamics, and the groundwater signature through time. At its conclusion, insights gained from this study will be used by municipal authorities to refine protective zones around pumping wells and to direct resources towards updating practices and replacing

Thermodynamic compensation upon binding to exosite 1 and the active site of thrombin.

Science.gov (United States)

Treuheit, Nicholas A; Beach, Muneera A; Komives, Elizabeth A

2011-05-31

Several lines of experimental evidence including amide exchange and NMR suggest that ligands binding to thrombin cause reduced backbone dynamics. Binding of the covalent inhibitor dPhe-Pro-Arg chloromethyl ketone to the active site serine, as well as noncovalent binding of a fragment of the regulatory protein, thrombomodulin, to exosite 1 on the back side of the thrombin molecule both cause reduced dynamics. However, the reduced dynamics do not appear to be accompanied by significant conformational changes. In addition, binding of ligands to the active site does not change the affinity of thrombomodulin fragments binding to exosite 1; however, the thermodynamic coupling between exosite 1 and the active site has not been fully explored. We present isothermal titration calorimetry experiments that probe changes in enthalpy and entropy upon formation of binary ligand complexes. The approach relies on stringent thrombin preparation methods and on the use of dansyl-l-arginine-(3-methyl-1,5-pantanediyl)amide and a DNA aptamer as ligands with ideal thermodynamic signatures for binding to the active site and to exosite 1. Using this approach, the binding thermodynamic signatures of each ligand alone as well as the binding signatures of each ligand when the other binding site was occupied were measured. Different exosite 1 ligands with widely varied thermodynamic signatures cause a similar reduction in ΔH and a concomitantly lower entropy cost upon DAPA binding at the active site. The results suggest a general phenomenon of enthalpy-entropy compensation consistent with reduction of dynamics/increased folding of thrombin upon ligand binding to either the active site or exosite 1.

Getting ready for inspection of investigational site at short notice

Directory of Open Access Journals (Sweden)

Rajendra Talele

2010-01-01

Full Text Available India is becoming an attractive destination for drug development and clinical research. This is evidenced by the three fold increment in clinical trial applications in last four years to the office of Drugs Controller General of India (DCGI. This upward trend is collaborative efforts of all stake holders and the quality of Indian data. Therefore to sustain this trend, it is important that stake holders such as Regulators, Sponsor, CRO, Monitor, Investigators and trial subjects required maintaining high standards of data and conduct of clinical trials. Indian regulations and the role of DCGI in quality check for Indian clinical trials is always a topic of discussion in various forums. A recent move by DCGI for conducting random inspections of investigational sites and companies at short notice, checking their compliance in accordance with the guidelines, and taking action against non-complier is welcomed. This will certainly increase over quality of the clinical trials. Quality of clinical trial conduct is measured on essential documents for their appropriateness and its correctness. It is observed that the stakeholders engaged in multitasking often overlook the requirements or appropriateness of the document due to their focused approach on a specific activity which is on priority. This can lead to serious quality problem and issues. Understanding of the process and documents reviewed by auditor is important to maintain such high quality. The proper planning and time management working on essential documents can minimize the quality issues, and we can be always ready for any type of inspection, announced or unannounced, or "short notice".

The origins of enhanced activity in factor VIIa analogs and the interplay between key allosteric sites revealed by hydrogen exchange mass spectrometry

DEFF Research Database (Denmark)

Rand, Kasper D; Andersen, Mette D; Olsen, Ole H

2008-01-01

Factor VIIa (FVIIa) circulates in the blood in a zymogen-like state. Only upon association with membrane-bound tissue factor (TF) at the site of vascular injury does FVIIa become active and able to initiate blood coagulation. Here we used hydrogen exchange monitored by mass spectrometry to invest......Factor VIIa (FVIIa) circulates in the blood in a zymogen-like state. Only upon association with membrane-bound tissue factor (TF) at the site of vascular injury does FVIIa become active and able to initiate blood coagulation. Here we used hydrogen exchange monitored by mass spectrometry...... to investigate the conformational effects of site-directed mutagenesis at key positions in FVIIa and the origins of enhanced intrinsic activity of FVIIa analogs. The differences in hydrogen exchange of two highly active variants, FVIIa(DVQ) and FVIIa(VEAY), imply that enhanced catalytic efficiency was attained...

Active-Site Hydration and Water Diffusion in Cytochrome P450cam: A Highly Dynamic Process

Energy Technology Data Exchange (ETDEWEB)

Miao, Yinglong [ORNL; Baudry, Jerome Y [ORNL

2011-01-01

Long-timescale molecular dynamics simulations (300 ns) are performed on both the apo- (i.e., camphor-free) and camphor-bound cytochrome P450cam (CYP101). Water diffusion into and out of the protein active site is observed without biased sampling methods. During the course of the molecular dynamics simulation, an average of 6.4 water molecules is observed in the camphor-binding site of the apo form, compared to zero water molecules in the binding site of the substrate-bound form, in agreement with the number of water molecules observed in crystal structures of the same species. However, as many as 12 water molecules can be present at a given time in the camphor-binding region of the active site in the case of apo-P450cam, revealing a highly dynamic process for hydration of the protein active site, with water molecules exchanging rapidly with the bulk solvent. Water molecules are also found to exchange locations frequently inside the active site, preferentially clustering in regions surrounding the water molecules observed in the crystal structure. Potential-of-mean-force calculations identify thermodynamically favored trans-protein pathways for the diffusion of water molecules between the protein active site and the bulk solvent. Binding of camphor in the active site modifies the free-energy landscape of P450cam channels toward favoring the diffusion of water molecules out of the protein active site.

Resource Conservation and Recovery Act Industrial Sites quality assurance project plan: Nevada Test Site, Nevada

International Nuclear Information System (INIS)

1994-06-01

This quality assurance project plan (QAPjP) describes the measures that shall be taken to ensure that the environmental data collected during characterization and closure activities of Resource Conservation and Recovery Act (RCRA) Industrial Sites at the Nevada Test Site (NTS) are meaningful, valid, defensible, and can be used to achieve project objectives. These activities are conducted by the US Department of Energy Nevada Operations Office (DOE/NV) under the Nevada Environmental Restoration (ER) Project. The Nevada ER Project consists of environmental restoration activities on the NTS, Tonopah Test Range, Nellis Air Force Range, and eight sites in five other states. The RCRA Industrial Sites subproject constitutes a component of the Nevada ER Project. Currently, this QAPjP is limited to the seven RCRA Industrial Sites identified within this document that are to be closed under an interim status and pertains to all field- investigation, analytical-laboratory, and data-review activities in support of these closures. The information presented here supplements the RCRA Industrial Sites Project Management Plan and is to be used in conjunction with the site-specific subproject sampling and analysis plans

Active site mutations change the cleavage specificity of neprilysin.

Directory of Open Access Journals (Sweden)

Travis Sexton

Full Text Available Neprilysin (NEP, a member of the M13 subgroup of the zinc-dependent endopeptidase family is a membrane bound peptidase capable of cleaving a variety of physiological peptides. We have generated a series of neprilysin variants containing mutations at either one of two active site residues, Phe(563 and Ser(546. Among the mutants studied in detail we observed changes in their activity towards leucine(5-enkephalin, insulin B chain, and amyloid β(1-40. For example, NEP(F563I displayed an increase in preference towards cleaving leucine(5-enkephalin relative to insulin B chain, while mutant NEP(S546E was less discriminating than neprilysin. Mutants NEP(F563L and NEP(S546E exhibit different cleavage site preferences than neprilysin with insulin B chain and amyloid ß(1-40 as substrates. These data indicate that it is possible to alter the cleavage site specificity of neprilysin opening the way for the development of substrate specific or substrate exclusive forms of the enzyme with enhanced therapeutic potential.

Corrective Action Investigation Plan for Corrective Action Unit 542: Disposal Holes, Nevada Test Site, Nevada

International Nuclear Information System (INIS)

Laura Pastor

2006-01-01

Corrective Action Unit (CAU) 542 is located in Areas 3, 8, 9, and 20 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 542 is comprised of eight corrective action sites (CASs): (1) 03-20-07, ''UD-3a Disposal Hole''; (2) 03-20-09, ''UD-3b Disposal Hole''; (3) 03-20-10, ''UD-3c Disposal Hole''; (4) 03-20-11, ''UD-3d Disposal Hole''; (5) 06-20-03, ''UD-6 and UD-6s Disposal Holes''; (6) 08-20-01, ''U-8d PS No.1A Injection Well Surface Release''; (7) 09-20-03, ''U-9itsy30 PS No.1A Injection Well Surface Release''; and (8) 20-20-02, ''U-20av PS No.1A Injection Well Surface Release''. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on January 30, 2006, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and Bechtel Nevada. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 542. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to each CAS. The scope of the CAI for CAU 542 includes the following activities: (1) Move surface debris and/or materials, as needed, to facilitate sampling. (2) Conduct radiological surveys. (3) Conduct geophysical surveys to

Shemya AFB, Alaska 1992 IRP field investigation report

Energy Technology Data Exchange (ETDEWEB)

1993-02-01

The US Air Force is currently investigating 22 sites on Shemya Air Force Base (AFB) to determine if past spill and disposal activities have caused environmental damage. These investigations are being carried out under the Air Force's Installation Restoration Program (IRP). Field investigations were performed in 1992 to obtain the information needed to assess what future actions will need to be carried out at each site. The island's drinking water supply was also investigated. Activities completed at 10 selected sites included surface sampling to determine the lateral extent of contamination, subsurface sampling to determine the vertical extent of contamination, and the installation of well points and monitoring wells to determine the direction of groundwater flow and if the groundwater has been affected by a site. Geophysical surveys were performed at most sites to identify site boundaries and check for the presence of buried metal to be avoided during drilling activities. This report, appendices B, C, and D contains information on the following: geophysical contour maps and profile plots; human health risk assessment; and ecological risk assessment.

Shemya AFB, Alaska 1992 IRP field investigation report

Energy Technology Data Exchange (ETDEWEB)

1993-02-01

The US Air Force is currently investigating 22 sites on Shemya Air Force Base (AFB) to determine if past spill and disposal activities have caused environmental damage. These investigations are being carried out under the Air Force's Installation Restoration Program (IRP). Field investigations were performed in 1992 to obtain the information needed to assess what future actions will need to be carried out at each site. The island's drinking water supply was also investigated. Activities completed at 10 selected sites included surface sampling to determine the lateral extent of contamination, subsurface sampling to determine the vertical extent of contamination, and the installation of well points and monitoring wells to determine the direction of groundwater flow and if the groundwater has been affected by a site. In addition, geophysical surveys were performed at most sites to identify site boundaries and check for the presence of buried metal, to be avoided during drilling activities. This report contains appendices E and F with information on the following: soil boring logs, and data validation of samples analyzed.

Savannah River Site RCRA/CERCLA/NEPA integrated investigation case study

International Nuclear Information System (INIS)

Clark, D.R.; Thomas, R.; Wilson, M.P.

1992-01-01

The Savannah River Site (SRS) is a US Department of Energy facility placed on the Superfund National Priority List in 1989. Numerous past disposal facilities and contaminated areas are undergoing the integrated regulatory remediation process detailed in the draft SRS Federal Facility Agreement. This paper will discuss the integration of these requirements by highlighting the investigation of the D-Area Burning/Rubble Pits, a typical waste unit at SRS

Remedial investigation/feasibility study for the David Witherspoon, Inc., 901 Site, Knoxville, Tennessee: Volume 1

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-10-01

This remedial investigation (RI)/feasibility study (FS) supports the selection of remedial actions for the David Witherspoon, Inc. 901 Maryville Pike Site in Knoxville, Tennessee. Operations at the site, used as a recycling center, have resulted in past, present, and potential future releases of hazardous substances in to the environment. This Site is a Tennessee Superfund site. A phased approach was planned to (1) gather existing data from previous investigations managed by the Tenn. Dept. of Environment and Conservation; (2) perform a preliminary RI, including risk assessments, and an FS with existing data to identify areas where remedial action may be necessary; (3) gather additional field data to adequately define the nature and extent of risk-based contaminants that present identifiable threats to human and/or ecological receptors; and (4) develop remedial action alternatives to reduce risks to acceptable levels.

Remedial investigation/feasibility study for the David Witherspoon, Inc., 901 Site, Knoxville, Tennessee: Volume 1

International Nuclear Information System (INIS)

1996-10-01

This remedial investigation (RI)/feasibility study (FS) supports the selection of remedial actions for the David Witherspoon, Inc. 901 Maryville Pike Site in Knoxville, Tennessee. Operations at the site, used as a recycling center, have resulted in past, present, and potential future releases of hazardous substances in to the environment. This Site is a Tennessee Superfund site. A phased approach was planned to (1) gather existing data from previous investigations managed by the Tenn. Dept. of Environment and Conservation; (2) perform a preliminary RI, including risk assessments, and an FS with existing data to identify areas where remedial action may be necessary; (3) gather additional field data to adequately define the nature and extent of risk-based contaminants that present identifiable threats to human and/or ecological receptors; and (4) develop remedial action alternatives to reduce risks to acceptable levels

Experimental warming differentially affects microbial structure and activity in two contrasted moisture sites in a Sphagnum-dominated peatland.

Science.gov (United States)

Delarue, Frédéric; Buttler, Alexandre; Bragazza, Luca; Grasset, Laurent; Jassey, Vincent E J; Gogo, Sébastien; Laggoun-Défarge, Fatima

2015-04-01

Several studies on the impact of climate warming have indicated that peat decomposition/mineralization will be enhanced. Most of these studies deal with the impact of experimental warming during summer when prevalent abiotic conditions are favorable to decomposition. Here, we investigated the effect of experimental air warming by open-top chambers (OTCs) on water-extractable organic matter (WEOM), microbial biomasses and enzymatic activities in two contrasted moisture sites named Bog and Fen sites, the latter considered as the wetter ones. While no or few changes in peat temperature and water content appeared under the overall effect of OTCs, we observed that air warming smoothed water content differences and led to a decrease in mean peat temperature at the warmed Bog sites. This thermal discrepancy between the two sites led to contrasting changes in microbial structure and activities: a rise in hydrolytic activity at the warmed Bog sites and a relative enhancement of bacterial biomass at the warmed Fen sites. These features were not associated with any change in WEOM properties namely carbon and sugar contents and aromaticity, suggesting that air warming did not trigger any shift in OM decomposition. Using various tools, we show that the use of single indicators of OM decomposition can lead to fallacious conclusions. Lastly, these patterns may change seasonally as a consequence of complex interactions between groundwater level and air warming, suggesting the need to improve our knowledge using a high time-resolution approach. Copyright © 2015 Elsevier B.V. All rights reserved.

The use of small diameter probing equipment for contaminated site investigation

International Nuclear Information System (INIS)

Christy, T.M.; Spradlin, S.C.

1992-01-01

The past decade has witnessed a dramatic increase in the number of contaminated sites being investigated in the United States. This increase in subsurface investigation has spurred a corresponding increase in the development of subsurface sampling tools and methods. The past five years, in particular, have seen the development of small diameter (1 double-prime to 1.4 double-prime O.D.) percussion driven probing tools which can be used for the recovery of soil vapor, soil core and groundwater samples. This development has placed heretofore unavailable tools at the disposal of site investigators. Mechanized, vehicle mounted soil probe systems apply both static force and hydraulically powered percussion hammers for tool placement. Static down forces up to 3,000 lbs combined with percussion hammers of eight (8) horsepower continuous output are typical on equipment available to the field investigator. Using these energies, probing tools have been used for sampling a variety of media at depths exceeding 70 feet. Advantages of probing equipment which have contributed to its increasing usage in recent years include: ease of mobilization, absence of borehole cuttings, minimization of surface disturbance, and speed of sample collection. This paper focuses on the field application of hydraulic probing equipment including: the suitability of probing operations with respect to various Boil types and lithologies to probing operations; sampler types and recovery quantities for various media, and innovative probing applications presently being tested

Drilling to investigate processes in active tectonics and magmatism

Science.gov (United States)

Shervais, J.; Evans, J.; Toy, V.; Kirkpatrick, J.; Clarke, A.; Eichelberger, J.

2014-12-01

Coordinated drilling efforts are an important method to investigate active tectonics and magmatic processes related to faults and volcanoes. The US National Science Foundation (NSF) recently sponsored a series of workshops to define the nature of future continental drilling efforts. As part of this series, we convened a workshop to explore how continental scientific drilling can be used to better understand active tectonic and magmatic processes. The workshop, held in Park City, Utah, in May 2013, was attended by 41 investigators from seven countries. Participants were asked to define compelling scientific justifications for examining problems that can be addressed by coordinated programs of continental scientific drilling and related site investigations. They were also asked to evaluate a wide range of proposed drilling projects, based on white papers submitted prior to the workshop. Participants working on faults and fault zone processes highlighted two overarching topics with exciting potential for future scientific drilling research: (1) the seismic cycle and (2) the mechanics and architecture of fault zones. Recommended projects target fundamental mechanical processes and controls on faulting, and range from induced earthquakes and earthquake initiation to investigations of detachment fault mechanics and fluid flow in fault zones. Participants working on active volcanism identified five themes: the volcano eruption cycle; eruption sustainability, near-field stresses, and system recovery; eruption hazards; verification of geophysical models; and interactions with other Earth systems. Recommended projects address problems that are transferrable to other volcanic systems, such as improved methods for identifying eruption history and constraining the rheological structure of shallow caldera regions. Participants working on chemical geodynamics identified four major themes: large igneous provinces (LIPs), ocean islands, continental hotspot tracks and rifts, and