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Sample records for sistema hibrido fv

  1. Analysis tools for simulation of hybrid systems; Herramientas de analisis para simulacion de sistemas hibridos

    Energy Technology Data Exchange (ETDEWEB)

    Guillen S, Omar; Mejia N, Fortino [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)

    2005-07-01

    In order to facilitate and to simplify the development and analysis of a Hybrid System in reference to its design, construction, operation and maintenance, it turns out optimal to carry out the simulation of this one by means of software, with which a significant reduction in the investment costs is obtained. Given the mix of technology of electrical generation which is involved in a hybrid system, it is very important to have a tool integrated with specialized packages of calculation (software), that allow to carry out the simulation tasks of the operational functioning of these systems. Combined with the former, one must not fail to consider the operation characteristics, the facilities of the user, the clarity in the obtained results and the possibility of its validation with respect to prototypes orchestrated in field. Equally, it is necessary to consider the identification of tasks involved in relation to the place of installation of this electrification technology. At the moment, the hybrid systems technology still is in a stage of development in the international level, and exist important limitations as far as the methodology availability and engineering tools for the optimum design of these systems. With the development of this paper, it is intended to contribute to the advance of the technology and to count on own tools to solve the described series of problems. In this article are described the activities that more impact have in the design and development of hybrid systems, as well as the identification of variables, basic characteristics and form of validation of tools in the integration of a methodology for the simulation of these systems, facilitating their design and development. [Spanish] Para facilitar y simplificar el desarrollo y analisis de un Sistema Hibrido en lo que refiere a su diseno, construccion, operacion y mantenimiento, resulta optimo efectuar la simulacion de este por medio de un software, con lo que se obtiene una reduccien

  2. Battery monitoring in Mexican hybrid power systems; Monitoreo de las baterias en sistemas de potencia hibridos Mexicanos

    Energy Technology Data Exchange (ETDEWEB)

    Flores, J. Roberto; Agredano, Jaime [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1997-12-31

    Hybrid power systems for an autonomous power supply are based on different renewable and fossil energy sources. They are considered as a good option for the power supply of remote areas. In these systems an energy storage is a vital necessity and very often this storage will consist of batteries which are generally connected in series and parallel arrays, or both. In Mexico as in other countries, the most extensively use batteries used for this application are the stationary and electric car type deep cycle batteries. However the experience with them in these systems is generally not very good. One way to overcome this problem is to maintain a regular monitoring of installing monitoring equipment, in order to make preventive actions before a developing fault can have serious consequences and in this manner increase the practical lifetime of the batteries. Unfortunately, battery monitoring is not easy task because most of the hybrid power systems are installed in remote areas which makes it difficult and expensive. In Mexico it has been not possible to maintain a regular monitoring of all hybrid power systems installed, due to the high cost of this work and the lack of founds. The hybrid power systems installed in the state of Quintana Roo are the only systems that have been continuously monitored since their installation. This paper gives an overview of the hybrid power systems installed in Mexico, focusing in the battery banks, the way they are being monitored, the main parameters used to detect possible premature problems and the method used to evaluate the battery bank conditions. Finally some results from the battery banks monitoring activities are presented. [Espanol] Los sistemas de potencia hibridos para un suministro autonomo de energia a regiones remotas, estan basados en diferentes fuentes de energia fosiles y renovables. Estos son considerados como una buena opcion para el suministro de energia a areas remotas. En estos sistemas es una necesidad vital el

  3. Battery monitoring in Mexican hybrid power systems; Monitoreo de las baterias en sistemas de potencia hibridos Mexicanos

    Energy Technology Data Exchange (ETDEWEB)

    Flores, J Roberto; Agredano, Jaime [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1998-12-31

    Hybrid power systems for an autonomous power supply are based on different renewable and fossil energy sources. They are considered as a good option for the power supply of remote areas. In these systems an energy storage is a vital necessity and very often this storage will consist of batteries which are generally connected in series and parallel arrays, or both. In Mexico as in other countries, the most extensively use batteries used for this application are the stationary and electric car type deep cycle batteries. However the experience with them in these systems is generally not very good. One way to overcome this problem is to maintain a regular monitoring of installing monitoring equipment, in order to make preventive actions before a developing fault can have serious consequences and in this manner increase the practical lifetime of the batteries. Unfortunately, battery monitoring is not easy task because most of the hybrid power systems are installed in remote areas which makes it difficult and expensive. In Mexico it has been not possible to maintain a regular monitoring of all hybrid power systems installed, due to the high cost of this work and the lack of founds. The hybrid power systems installed in the state of Quintana Roo are the only systems that have been continuously monitored since their installation. This paper gives an overview of the hybrid power systems installed in Mexico, focusing in the battery banks, the way they are being monitored, the main parameters used to detect possible premature problems and the method used to evaluate the battery bank conditions. Finally some results from the battery banks monitoring activities are presented. [Espanol] Los sistemas de potencia hibridos para un suministro autonomo de energia a regiones remotas, estan basados en diferentes fuentes de energia fosiles y renovables. Estos son considerados como una buena opcion para el suministro de energia a areas remotas. En estos sistemas es una necesidad vital el

  4. Supervisory control for hybrid systems of electrical generation based on fuzzy logic; Control supervisorio para sistemas hibridos de generacion electrica basado en logica difusa

    Energy Technology Data Exchange (ETDEWEB)

    Lagunas M, Javier; Ortega S, Cesar [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico); Caratozzolo M, Patricia [Instituto Tecnologico de Estudios Superiores de Monterrey, campus Cd. de Mexico (Mexico)

    2005-07-01

    The development and integration of hybrid systems of electrical generation (SHGE) of small capacity: The intention of these developments is its implementation in isolated or far away communities from conventional electric networks, that contribute in the own productive processes of these towns. As part of these work a system of control for their hybrid system wind-photovoltaic- internal combustion machine was developed that operates nowadays in a system installed in Pachuca, Hidalgo, Mexico. However, in the two past years, the GENC has worked, altogether with the Management of Control and Instrumentation of the Instituto de Investigaciones Electricas (IIE) and the Centro Nacional de Investigacion y Desarrollo Tecnologico (CENIDET) (National Center of Research and Technological Development), to incorporate an intelligent control technique in the regulation of the hybrid systems of wind-photovoltaic-machine of internal combustion type. Lemos de Pereira rises that the main problems of the present technology of the SHGE are related to the control and supervision of the power systems. The system that is in charge of the actions of load control and dispatch is denominated supervisory control. This controller supervises the operation of all the components, regulates the entry or exiting of operation of the generation systems, as well as the loads. [Spanish] El desarrollo e integracion de sistemas hibridos de generacion electrica (SHGE) de pequena capacidad. El proposito de estos desarrollos es su implementacion en comunidades aisladas o alejadas de la red electrica convencional, las cuales contribuyan en los procesos productivos propios de estos poblados. Como parte de dichos trabajos se desarrollo un sistema de control para su sistema hibrido eolico-fotovoltaico-maquina de combustion interna que opera actualmente en un sistema instalado en Pachuca, Hidalgo, Mexico. Ahora bien, en los dos ultimos anos, la GENC ha trabajado, en conjunto con la Gerencia de Control e

  5. Supervisory fussy control for an electric generating hybrid system; Control supervisorio difuso para un sistema hibrido de generacion electrica

    Energy Technology Data Exchange (ETDEWEB)

    Lagunas Mendoza, Javier

    2004-11-15

    the fuzzy supervisory controller were compared with a conventional controller. The results shows that the supervisory controller fulfills the three main objectives of the system operation: I) to reduce the fuel consumption of the back-up system, II) to reduce the depth of the discharge of the battery bank, and III) to keep fully charged the battery bank as much as possible in order to make a better use of the available free renewable energy sources. [Spanish] En este trabajo se presenta el desarrollo de un controlador supervisorio difuso para un sistema hibrido de generacion electrica (SHGE). En este trabajo se definio la configuracion y los elementos que componen el SHGE a utilizar, producto de la revision de configuraciones de sistemas hibridos propuestas actualmente. La configuracion del SHGE incluye: arreglos fotovoltaicos y aerogeneradores como fuentes de generacion renovable, una maquina de combustion interna como sistema de respaldo, un banco de baterias como medio de almacenamiento de energia, cargas electricas en corriente alterna y un inversor CD/CA para poder alimentarlas. Tambien a partir de la configuracion propuesta se buscaron y seleccionaron los modelos matematicos de los diferentes elementos del sistema. Se llevo a cabo la validacion de los modelos a traves de la comparacion de la salida contra datos reales obtenidos en un SHGE instalado en Pachuca. Con los modelos se llevo a cabo el desarrollo de un programa de simulacion para un SHGE en Matlab. Se determino la funcion principal del controlador supervisorio dentro del SHGE. La funcion es satisfacer el o los objetivos de operacion del sistema. La filosofia de operacion del controlador supervisorio se definio a partir de la configuracion electrica propuesta y de los objetivos de operacion del sistema. Por lo que respecta al desarrollo del controlador supervisorio, se llevo a cabo una revision de los controladores supervisorios actuales que utilizan alguna tecnica de control inteligente. A partir de

  6. Hybrid system: Heat pump-solar air dryer for grains; Sistema hibrido: bomba de calor - calentador solar de aire para el secado de productos agricolas

    Energy Technology Data Exchange (ETDEWEB)

    Soto Gomez, Willfredo [Instituto Tecnologico de Tijuana, Tijuana (Mexico); Ortega Herrera, Jose Angel [Instituto Politecnico Nacional, Mexico, D.F. (Mexico)

    2000-07-01

    Design, building, operation and evaluation energy wise of a hybrid experimental type, with heat pump, that uses no chloride, does not destroy the ozone layer. It is solar air dryer for grains. In this research we dry rice. It has tree systems: 1.- A mechanical compression heat pump, 2.- An air solar heater, and 3.- An agriculture products dryer. The drying capacity is 20 pounds of grain /day, with a median daily solar radiation. The costs is approximately U.S. $ 6 000.00. The heat pump used 22 refrigerant first, and now works with refrigerant SUVA 9000. This refrigerant will be available this year in the I.S., it is one of the ecological class that substitutes the chlorofluorocarbonates. [Spanish] Se disena, construye, opera, y evalua energeticamente, un sistema hibrido tipo experimental, con bomba de calor que utiliza refrigerante que no contiene cloro, y no destruye la capa de ozono y un calentador solar de aire, para secar granos. En este trabajo secamos arroz. Se compone de tres sistemas: 1.- Bomba de calor por compresion mecanica, 2.- Calentador solar de aire, 3.- Secador de productos agricolas. La capacidad de secado es de 10 Kilos de granos/dia promedio. Tiene un costo aproximado de $ 60 000. La bomba de calor utiliza refrigerante 22 en una primera generacion, y actualmente opera con un refrigerante SUVA 9000, en una segunda generacion, este refrigerante se comercializara en este ano, en la Union Americana, pertenece a la familia de los llamados refrigerantes ecologicos, sustitutos de los clorofluorocarbonados.

  7. Hybrid 95: Software for the design of hybrid systems, Potrerillo of Santa Teresa; Hybrid 95: Software de diseno de sistemas hibridos, Potrerillo de Santa Teresa

    Energy Technology Data Exchange (ETDEWEB)

    Casaravilla, G.; Chaer, R. [Instituto de Ingenieria Electrica, Facultad de Ingenieria, Universidad de la Republica, Montevideo (Uruguay)

    1997-12-31

    This paper describes the software Hybrid 95 and how it is used for the design of hybrid systems. As an application example some of the design stages are looked over (determination of the wind resource, simulations for sizing generation/storage and topology) of the Potrerillo installation. Taking into account the site clear ecological connotations, during the design consideration is made of the visual impact that represents the possible wind-generator, therefore several locations and wind-generator heights are studied, even arriving to the wind-generation elimination. [Espanol] Este trabajo describe el software Hybrid 95 y como se utiliza a los efectos del diseno de sistemas hibridos. Como ejemplo de aplicacion se recorren algunas de las etapas del diseno (determinacion del recurso eolico, simulaciones para el dimensionado de generacion/acumulacion y topologia) de la instalacion de Potrerillo. Teniendo en cuenta las claras connotaciones ecologistas del lugar, durante el diseno se tiene presente el impacto visual que representa el eventual aerogenerador por lo que se estudian diversas ubicaciones y alturas de aerogenerador, llegandose incluso a estudiar la eliminacion de la generacion eolica.

  8. Hybrid 95: Software for the design of hybrid systems, Potrerillo of Santa Teresa; Hybrid 95: Software de diseno de sistemas hibridos, Potrerillo de Santa Teresa

    Energy Technology Data Exchange (ETDEWEB)

    Casaravilla, G; Chaer, R [Instituto de Ingenieria Electrica, Facultad de Ingenieria, Universidad de la Republica, Montevideo (Uruguay)

    1998-12-31

    This paper describes the software Hybrid 95 and how it is used for the design of hybrid systems. As an application example some of the design stages are looked over (determination of the wind resource, simulations for sizing generation/storage and topology) of the Potrerillo installation. Taking into account the site clear ecological connotations, during the design consideration is made of the visual impact that represents the possible wind-generator, therefore several locations and wind-generator heights are studied, even arriving to the wind-generation elimination. [Espanol] Este trabajo describe el software Hybrid 95 y como se utiliza a los efectos del diseno de sistemas hibridos. Como ejemplo de aplicacion se recorren algunas de las etapas del diseno (determinacion del recurso eolico, simulaciones para el dimensionado de generacion/acumulacion y topologia) de la instalacion de Potrerillo. Teniendo en cuenta las claras connotaciones ecologistas del lugar, durante el diseno se tiene presente el impacto visual que representa el eventual aerogenerador por lo que se estudian diversas ubicaciones y alturas de aerogenerador, llegandose incluso a estudiar la eliminacion de la generacion eolica.

  9. Feasibility of implementation of an autonomous hybrid system for PEM fuel cells to electrify localities in rural Cuba; Factibilidad de implementacion de un sistema hibrido autonomo con celda de combustible PEM para electrificar localidades rurales en Cuba

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez Torres, Yamir [Centro de Estudios de Tecnologias Energeticas Renovables (CETER), Ciudad de la Habana (Cuba)] e-mail: yamir@ceter.cujae.edu.cu

    2009-09-15

    The use of PEM fuel cells to produce electric energy in autonomous systems is closely linked with the production and storage of hydrogen. Eventually joined with sources of renewable energy, this creates an ecologically clean and sustainable system. In several developing countries, localities exist that do not have electricity but have significant unexploited renewable energy power, where an autonomous hybrid system can be designed to electrify these population centers. This work presents a hybrid electricity scheme with a PEM fuel cell to produce hydrogen and electricity in order to electrify rural zones far from the national power grid in Cuba. The electric demand of the zone and available energy power was calculated using the informatics modeling and simulation programs HOMER, PVSYST and Matlab 1,2,3. Variability in wind and photovoltage power was determined based on daylight hours and seasonal periods throughout the year as well as their effect on the production of hydrogen and electricity. It was shown that the energy demand is met even for the most adverse scenarios. This work offers a detailed description of the behavior of the system and evidence of no effect on the environment, enabling the electrification and wellbeing of residents of the locality. [Spanish] El uso de celdas de combustible PEM para la produccion de energia electrica en sistemas autonomos esta estrechamente ligado a la produccion y almacenamiento de hidrogeno. Esto eventualmente unido a las fuentes renovables de energia forma un sistema ecologicamente limpio y sustentable. En varios paises subdesarrollados existen localidades que no cuentan con electricidad y que tienen importantes potenciales energeticos renovables no explotados actualmente en los cuales se puede disenar un sistema hibrido autonomo para electrificar estas poblaciones. En este trabajo se presenta el esquema de un sistema hibrido autonomo con celda de combustible PEM, para la produccion de hidrogeno y electricidad encaminado

  10. Temperature control of a steam generator by means of an hybrid system PID-RLC; Control de las temperaturas de un generador de vapor mediante un sistema hibrido PID-RLC

    Energy Technology Data Exchange (ETDEWEB)

    Palomares Gonzalez, Daniel; Garcia Mendoza, Raul [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1990-12-31

    A description is made of the design and evaluation of an hybrid control system, formed by a quadratic gaussian linear regulator (QLR) and proportional integral derivative (PID) type regulators. This scheme is used to control the reheater and secondary superheater steam temperatures of a steam generator model with a maximum capacity of 2,150,000 pounds per hour. Once applied to the model of a 300 MW steam power plant, this system showed better results than the traditional schemes and inclusively better than some modern control schemes. This fact characterizes it as a high potential system to be applied to steam power plants. [Espanol] Se describe el diseno y la evaluacion de un sistema de control hibrido, formado por un regulador lineal cuadratico gaussiano (RLC) y reguladores tipo proporcional integral derivativo (PID). Este esquema se utiliza para controlar las temperaturas de vapor del recalentador y sobrecalentador secundario del modelo de un generador de vapor con capacidad maxima de 2,150,000 libras por hora. Una vez aplicado al modelo de una unidad termoelectrica de 300 MW, este sistema produjo mejores resultados que los esquemas tradicionales e incluso mejores que algunos esquemas de control moderno. Esto lo caracteriza como un sistema con un alto potencial para aplicarse a unidades termoelectricas.

  11. Temperature control of a steam generator by means of an hybrid system PID-RLC; Control de las temperaturas de un generador de vapor mediante un sistema hibrido PID-RLC

    Energy Technology Data Exchange (ETDEWEB)

    Palomares Gonzalez, Daniel; Garcia Mendoza, Raul [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1991-12-31

    A description is made of the design and evaluation of an hybrid control system, formed by a quadratic gaussian linear regulator (QLR) and proportional integral derivative (PID) type regulators. This scheme is used to control the reheater and secondary superheater steam temperatures of a steam generator model with a maximum capacity of 2,150,000 pounds per hour. Once applied to the model of a 300 MW steam power plant, this system showed better results than the traditional schemes and inclusively better than some modern control schemes. This fact characterizes it as a high potential system to be applied to steam power plants. [Espanol] Se describe el diseno y la evaluacion de un sistema de control hibrido, formado por un regulador lineal cuadratico gaussiano (RLC) y reguladores tipo proporcional integral derivativo (PID). Este esquema se utiliza para controlar las temperaturas de vapor del recalentador y sobrecalentador secundario del modelo de un generador de vapor con capacidad maxima de 2,150,000 libras por hora. Una vez aplicado al modelo de una unidad termoelectrica de 300 MW, este sistema produjo mejores resultados que los esquemas tradicionales e incluso mejores que algunos esquemas de control moderno. Esto lo caracteriza como un sistema con un alto potencial para aplicarse a unidades termoelectricas.

  12. Methodology for monitoring the behaviour of wind-photovoltaic hybrid system in the conditions of Cuba; Metodologia para el monitoreo del comportamiento de un sistema hibrido eolico-fotovoltaico en las condiciones de Cuba

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez G, Maria; Nunez, Ariel; Marquez M, Soe del C [Centro de Investigaciones de Energia Solar, Santiago de Cuba (Cuba)

    2000-07-01

    The proposal of a methodology is shown in the work that allows monitoring the behaviour of wind-photovoltaic hybrid system beginning with the study of the energy resources (wind and solar) of the known place, designed and put into operation a hybrid installation, using Text Processing techniques could obtained operation curves of the system daily, monthly and annual, to configure the reading for port series of the parameters measured during the evaluation of the system a denominated software HYBSYS was developed in Lab View for Windows 3.1 or superior. [Spanish] Se muestra la propuesta de una metodologia que permite monitorear el comportamiento de un sistema hibrido eolico-fotovoltaico, comenzando con el estudio de los recursos energeticos (eolico y solar) de un sitio conocido se diseno y puso en funcionamiento una instalacion hibrida, usando las tecnicas de un procesador se pudieron obtener las curvas de funcionamiento diaria, mensual y anual del sistema, para configurar la lectura por puerto serie de los parametros medidos durante la evaluacion del sistema se desarrollo un software denominado HYBSYS en Lab View para Windows 3.1 o superior.

  13. Efeito da genetica e dos sistemas de insensibilização eletrico e gasoso (CO2) no bem-estar e qualidade de carne de hibridos suinos.

    OpenAIRE

    William Bortoloni

    2005-01-01

    Resumo: Diversas pesquisas tem sido realizadas pela comunidade científica com o objetivo de avaliar o bem-estar de suínos durante o abate, entretanto, a maioria destes estudos não representam as condições de abate e constituição genética dos híbridos suínos produzidos e comercializados no Brasil. O presente estudo teve como objetivo principal, avaliar a influência da constituição genética de três híbridos suínos ( A, B e C) e dois sistemas de insensibilização (elétrico e gasoso) na qualidade ...

  14. Hybrid system of generating electricity, solar eolic diesel San Juanico, Baja California Sur, Mexico; Sistema hibrido de generacion electrica, eolico solar diesel San Juanico, Baja California Sur, Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Huerta, Javier [Comision Federal de Electricidad, La Paz, Baja California Sur (Mexico); Johnston, Peter [Technology Development, Arizona (United States); Napikoski, Chester [Generation Engineering, Arizona (United States); Escutia, Ricardo [Comision Federal de Electricidad, Baja California Sur (Mexico)

    2000-07-01

    that is generated thru the selling of electric energy, guarantees the sustainable operation of the system. [Spanish] La Comision Federal de Electricidad (CFE), y la empresa electrica norteamericana Arizona Public Service (APS), firmaron un convenio de colaboracion para desarrollar un proyecto de generacion de energia electrica con la utilizacion de energias renovables. Las premisas que se acordaron fueron las siguientes: 1. Enfoque del proyecto hacia una comunidad rural. 2. El costo de la obra debera ser menor al de su interconexion con el sistema convencional. 3. Aceptacion por parte de la comunidad, y autoridades gubernamentales. 4. Sustentabilidad de la operacion del sistema. Se efectuaron diversos analisis tecnicos y economicos asi como la evaluacion del recurso solar y eolico, estudio de impacto ambiental, plan de negocios para obtener la aprobacion de los recursos economicos ante instancias como Niagara Mohawk (NIMO), y la USAID, mediante la supervision de los Laboratorios Nacionales de Sandia. Despues de efectuarse las mediciones anemometricas y de radiacion solar, en diversas comunidades, se considero a la comunidad de San Juanico como la mas viable; tambien los aspectos de logistica, sociales, tamano de la comunidad y como detonador para las actividades economicas como la pesca y el turismo. La APS llevo a cabo el proyecto ejecutivo de conformidad con las recomendaciones de la Comision Federal de Electricidad. El proyecto consistio basicamente en la instalacion de 10 aerogeneradores de 10 Kw. c/u, un arreglo solar de 17 Kw., un inversor de 70 Kw., un banco de baterias de 432 Kwh., mas un motogenerador diesel de emergencia de 80 Kw. Ademas de la obra civil y electromecanica. Fue necesario involucrar a la poblacion en el conocimiento y seguimiento del proyecto desde sus inicios, ya que consideramos que este factor seria indispensable para lograr el exito del mismo. Se instalaron lamparas ahorradoras de energia en viviendas y alumbrado publico para optimizar el

  15. Hybrid system of generating electricity, solar eolic diesel San Juanico, Baja California Sur, Mexico; Sistema hibrido de generacion electrica, eolico solar diesel San Juanico, Baja California Sur, Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Huerta, Javier [Comision Federal de Electricidad, La Paz, Baja California Sur (Mexico); Johnston, Peter [Technology Development, Arizona (United States); Napikoski, Chester [Generation Engineering, Arizona (United States); Escutia, Ricardo [Comision Federal de Electricidad, Baja California Sur (Mexico)

    2000-07-01

    is generated thru the selling of electric energy, guarantees the sustainable operation of the system. [Spanish] La Comision Federal de Electricidad (CFE), y la empresa electrica norteamericana Arizona Public Service (APS), firmaron un convenio de colaboracion para desarrollar un proyecto de generacion de energia electrica con la utilizacion de energias renovables. Las premisas que se acordaron fueron las siguientes: 1. Enfoque del proyecto hacia una comunidad rural. 2. El costo de la obra debera ser menor al de su interconexion con el sistema convencional. 3. Aceptacion por parte de la comunidad, y autoridades gubernamentales. 4. Sustentabilidad de la operacion del sistema. Se efectuaron diversos analisis tecnicos y economicos asi como la evaluacion del recurso solar y eolico, estudio de impacto ambiental, plan de negocios para obtener la aprobacion de los recursos economicos ante instancias como Niagara Mohawk (NIMO), y la USAID, mediante la supervision de los Laboratorios Nacionales de Sandia. Despues de efectuarse las mediciones anemometricas y de radiacion solar, en diversas comunidades, se considero a la comunidad de San Juanico como la mas viable; tambien los aspectos de logistica, sociales, tamano de la comunidad y como detonador para las actividades economicas como la pesca y el turismo. La APS llevo a cabo el proyecto ejecutivo de conformidad con las recomendaciones de la Comision Federal de Electricidad. El proyecto consistio basicamente en la instalacion de 10 aerogeneradores de 10 Kw. c/u, un arreglo solar de 17 Kw., un inversor de 70 Kw., un banco de baterias de 432 Kwh., mas un motogenerador diesel de emergencia de 80 Kw. Ademas de la obra civil y electromecanica. Fue necesario involucrar a la poblacion en el conocimiento y seguimiento del proyecto desde sus inicios, ya que consideramos que este factor seria indispensable para lograr el exito del mismo. Se instalaron lamparas ahorradoras de energia en viviendas y alumbrado publico para optimizar el

  16. Modeling and control of an electric power generation hybrid system; Modelado y control de un sistema hibrido de generacion de energia electrica

    Energy Technology Data Exchange (ETDEWEB)

    Martinez Guzman, D; Aguilar Mejia, O; Tapia Olvera, R; Santiago Tepantlan, C. [Universidad Politecnica de Tulancingo, Huapalcalco, Hidalgo (Mexico)]. E-mail: omar.aguilar@upt.edu.mx

    2013-03-15

    With growing concerns on energy subject, the development of renewable energy sources is becoming more attractive. This paper presents the output power control of a wind energy conversion system based on a permanent magnet synchronous generator. The currents from voltage source inverter are controlled in a synchronous orthogonal dqo frame using a decoupled feed-forward control. Based on extensive simulation results using MATLAB/SIMULINK, it has been established that the performance of the controllers both in transient as well as in steady state is quite satisfactory. [Spanish] Con la creciente preocupacion en materia de energia, el desarrollo de fuentes de energia renovables es cada vez mas atractivo. Este trabajo presenta la regulacion de la potencia de salida de un sistema de generacion eolica basado en un generador sincrono de imanes permanentes. Las corrientes de la fuente inversora de voltaje son controladas en un marco de referencia ortogonal dqo usando un compensador por retroalimentacion en adelanto. Las simulaciones realizadas en MATLAB / Simulink, demuestran que los controladores presentan un excelente desempeno en estado transitorio, asi como en estado estacionario.

  17. Hybrid Fv - Biogas/Diesel System Measuring With Homer. Case of Application: Farm Pozo Verde, Jamundi Municipality, Cauca Valley, Colombia; Dimensionado de un Sistema Hibrido Fv - Biogas/Diesel mediante el Empleo de la Herramienta Homer. Caso de Aplicacion: Granja Pozo Verde, Municipio de Jamundi, Valle del Cauca, Colombia

    Energy Technology Data Exchange (ETDEWEB)

    Dominguez, J.; Manrique, P. A.; Pinedo, I.

    2009-11-25

    This report completes the previous one, by adding the characterization of renewable energy systems based on optimization and sizing tools. A case study has been carried out in the Valley of Cauca (Colombia) using HOMER, once the solar energy potential was defined. This work is about the production of electricity using hybrid power systems (HPS). The HPS chosen for this study is based on two of the most common energy resources in most of Colombian rural areas: solar energy and biomass waste. The biomass waste is obtained by animal manure from livestock farms, particularly from pigs, which is highly valued in terms of energy production. The breeding of pigs has a huge economic, environmental and social importance in this Colombian region. In this report, the HPS technology and the energy contribution of the biogas produced from animal manure are described. A technical and economic simulation of a HPS has been performed using the optimization tool HOMER. These reports are the result of a collaboration established between Universidad del Valle (Colombia) and CIEMAT (Spain), and have been done by the author while his staying at our center included on his PhD program. Keywords: renewable energies, hybrid systems, biogas, technology sizing, rural electrification, Latin America. (Author) 8 refs.

  18. sistemas

    Directory of Open Access Journals (Sweden)

    Diego Alejandro Muñoz Gaviria

    2006-01-01

    Full Text Available El texto presentado a continuación, plantea en términos generales las principales posturas conceptuales del sociólogo alemán Niklas Luhmann sobre las ideas de Max Weber y su teoría de la acción social, y de Talcott Parsons y sus planteamientos en torno a la teoría de los sistemas sociales. Al final del escrito se hace especial énfasis en el rol protagónico que para Luhmann tienen las ciencias sociales y humanas en la autorreferencia del sistema social.

  19. A hybrid solar/diesel water heating system: in medicine area in a hospital in Mexico city; Sistema hibrido de energia solar y diesel para calentamiento de agua: caso en el area de medicina fisica en un hospital en la ciudad de Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Nolasco Mejia, Miguel; Wolpert Kuri, Jorge [UNAM, Mexico, D.F. (Mexico)

    2000-07-01

    A hybrid solar/diesel water heating system for therapy in a hospital in Mexico is described in this paper. The use of solar energy in hospitals and clinics, represent an environmentally friendly alternative to the burning of fossil fuels. Analysis is made regarding the feasibility of integrating both solar energy and conventional energy (fuel oil) as back up, to satisfy the hot water demand for hydrotherapy in a hospital. Results from simulation show that solar energy is a cheap means to provide up to 60% of the hot water demand. The solar assisted system proposed uses flat plate solar collectors integrated with an existing hot water tank, where water is heated with vapor from a diesel ran boiler. This represents significant savings on the solar system cost. [Spanish] La utilizacion de la energia solar en hospitales y clinicas, representa una posibilidad para obtener ahorros importantes de energia para satisfacer la demanda de agua caliente. En el presente estudio se analiza la demanda de agua caliente para uso en medicina fisica (hidroterapia), en un hospital ubicado en la Cd. De Mexico y la factibilidad de usar la energia solar, empleando como apoyo el sistema tradicional de combustible fosil (diesel). Mediante un analisis termico se determina el calor necesario para satisfacer la demanda de agua caliente. El analisis economico muestra los costos del sistema solar, los ahorros que se tendran y el periodo de recuperacion de la inversion. Los resultados muestran que la utilizacion de la energia solar es factible debido a la disminucion tanto del consumo de combustible, como de las emisiones atmosfericas. Se propone la instalacion de un sistema solar con colectores planos y el aprovechamiento de uno de los dos tanques de agua caliente existentes, donde se efectua la trasferencia de calor al agua por medio de vapor, lo cual disminuye el costo del sistema solar. La aportacion solar puede ser mayor del 60%, el complemento sera aportado por el sistema tradicional con

  20. PROPIEDADES ELECTRONICAS DE SISTEMAS RESONANTES E HIBRIDOS MONOCAPA-BICAPA EN CINTAS DE GRAFENO

    OpenAIRE

    GONZALEZ SALAZAR, JHON WILFER; GONZALEZ SALAZAR, JHON WILFER

    2011-01-01

    La nanotecnología cada vez esta más integrada en nuestras vidas, los avances y sus alcances se incrementan día a día, para en poco tiempo, estar presente en casi todos los aspectos de nuestra vida cotidiana. En el sentido amplio de la definición, entendemos por nanotecnología a la tecnología basada en la manipulación de la materia en la escala dimensional del nanómetro, refiriéndose a la capacidad de realizar un sin fin de operaciones a escala atómica y molecular. Dado que este nuevo ti...

  1. Evaluation of operation strategies of hybrid systems; Avaliacao de estrategias de operacao de sistemas hibridos

    Energy Technology Data Exchange (ETDEWEB)

    Hauschild, Luciano; Zilles, Roberto [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Inst. de Eletrotecnica e Energia. Lab. de Sistemas Fotovoltaicos]. E-mail: lucianoh@iee.usp.br

    2006-07-01

    This article evaluates the operation strategies of photovoltaic-eolic-diesel hybrid systems used for the rural area applied to two cases studies placed at the Cardoso Island, SP. The operation strategies evaluation is performed by simulation using data collected at the Cardoso Island such as electric power consumption profile and the solar and wind potential. With those data, simulation have been done considering different operation strategies and different levels of renewable energies penetration in the total energy produced by the system, in order to obtain system operation results such as Diesel oil consumption and depth of average discharge of the battery banks. From the simulation results an economic analysis have been done by using elements data from the cost of life average cycle (LAC) for determining which strategy is the most adequate for operation the electric power supply hybrid system.

  2. Design and analysis of an intelligent public FV lighting system; Diseno y analisis de un alumbrado publico FV inteligente

    Energy Technology Data Exchange (ETDEWEB)

    Hanessian D, Ana V.; Gordon, Manuel [Universidad Autonoma Metropolitana, Unidad Azcapotzalco, Mexico, D.F. (Mexico)

    2009-07-01

    In the Mexico's National Energy Balance of 2008, it is considered that of the total of the electrical power consumption in our country, 18% is dedicated to lighting. Conscious of the necessity of saving energy in public lighting in this article is presented the design, construction and analysis of the power consumption of a public light fed with electricity of photovoltaic cells and the control of intensity on the light in inverse way of the natural light. A lamp constructed based in light emitting diodes (LEDs) is used. This has the quality of consuming very little energy that could be provided by the sun and be stored to use it at night. With this system, proven at scale, energy savings are obtained superior to 50% of the conventional one and, in relation to the commercial photovoltaic (FV) luminaries up to 30%. [Spanish] Del Balance Nacional de Energia, de Mexico, de 2008, se considera que del total del consumo de energia electrica en nuestro pais, el 18% esta dedicado a la iluminacion. Conscientes de la necesidad de ahorrar energia en alumbrado publico, en este articulo se presenta el diseno de construccion y analisis del consumo energetico de una luminaria publica alimentada con electricidad de celdas fotovoltaicas y el control de intensidad de la luz de manera inversa a la luz natural. Se utiliza un foco construido a base a los diodos emisores de luz (LEDs, por sus siglas en ingles). Estos tienen la cualidad de consumir muy poca energia que podra ser suministrada por el sol y almacenada para utilizarla en la noche. Con este sistema, probado a escala, se logran ahorros de energia superiores al 50% del convencional y, en relacion a las luminarias fotovoltaicas (FV) comerciales, hasa el 30%.

  3. Effective single chain antibody (scFv) concentrations in vivo via adenoviral vector mediated expression of secretory scFv

    NARCIS (Netherlands)

    Arafat, WO; Gomez-Navarro, J; Buchsbaum, DJ; Xiang, J; Casado, E; Barker, SD; Mahasreshti, PJ; Haisma, HJ; Barnes, MN; Siegal, GP; Alvarez, RD; Hemminki, A; Nettelbeck, DM; Curiel, DT

    Single chain antibodies (scFv) represent powerful interventional agents for the achievement of targeted therapeutics. The practical utility of these agents have been limited, however, by difficulties related to production of recombinant scFv and the achievement of effective and sustained levels of

  4. Cloning, bacterial expression and crystallization of Fv antibody fragments

    Science.gov (United States)

    E´, Jean-Luc; Boulot, Ginette; Chitarra, V´ronique; Riottot, Marie-Madeleine; Souchon, H´le`ne; Houdusse, Anne; Bentley, Graham A.; Narayana Bhat, T.; Spinelli, Silvia; Poljak, Roberto J.

    1992-08-01

    The variable Fv fragments of antibodies, cloned in recombinant plasmids, can be expressed in bacteria as functional proteins having immunochemical properties which are very similar or identical with those of the corresponding parts of the parent eukaryotic antibodies. They offer new possibilities for the study of antibody-antigen interactions since the crystals of Fv fragments and of their complexes with antigen reported here diffract X-rays to a higher resolution that those obtained with the cognate Fab fragments. The Fv approach should facilitate the structural study of the combining site of antibodies and the further characterization of antigen-antibody interactions by site-directed mutagenesis experiments.

  5. Study of a hybrid solar-electric oven to the curing composite materials used in aeronautics; Estudio de un horno hibrido solar-electrico para el curado de materiales compuestos utilizados en aeronautica

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez Roman, M. A.; Pineda Pinon, J.; Sanchez Sanchez, A. [CICATA - Unidad Queretaro, Santiago de Queretaro, Queretaro (Mexico)]. E-mail: mhernandezr0900@ipn.mx

    2010-11-15

    The study covers the curing of advanced composites in a hybrid solar electric oven. The furnace uses electricity from the grid power and solar energy provided by the heliostat concentrator system. The materials used in the experiments will be composite materials such as prepreg. The prepreg are fiberglass and carbon fiber. The resin used in the pre-impregnate will be epoxy resin. The work temperatures inside the furnace will be maximum 300 degrees Celsius. The obtained results will be useful to characterize the use of the solar energy and the characterize of the curing chamber. [Spanish] Se presenta el alcance para el estudio, el cual abarca el curado de materiales compuestos avanzados dentro de un horno hibrido solar electrico. El horno utilizara energia electrica suministrada por la red y energia solar suministrada por el sistema heliostato concentrador. Los materiales utilizados en los experimentos seran materiales compuestos tipo prepreg. Los prepreg seran de fibra de vidrio y de fibra de carbono. La resina utilizada en el preimpregando sera resina epoxica. Las temperaturas de trabajo dentro del horno seran como maximo de 300 grados centigrados. Los resultados obtenidos serviran para caracterizar el uso y aprovechamiento de la energia solar y la caracterizacion de la camara de curado.

  6. ATON - Informe sobre el estado del arte de la FV integrada en edificación, y productos y tecnologías FV actuales con enfoque en la tecnología de capa fina

    OpenAIRE

    Masseck, Torsten; Fonseca Casas, Antoni; Godoy Muñoz, Alfonso

    2012-01-01

    Informe sobre el estado del arte de la FV integrada en edificación en el marco del projecto ATON (CENIT-E), convenio UPC-ACCIONA Instalaciones El proyecto ATON en su Actividad 10: Integración arquitectónica persigue la búsqueda de conceptos innovadores para la integración de sistemas fotovoltaicos de lámina delgada o Thin-film en entornos urbanos, con énfasis en edificios. En colaboración con Acciona, el equipo de trabajo de la UPC ha realizado estudios de integraciones singulares de in...

  7. Hybrid solar-hydraulic electric power supply systems; Sistemas de fornecimento de energia eletrica hibrido solar hidraulico

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Doriana Marinho Novaes; Silva, Selenio Rocha; Alvim Filho, Aymore de Castro [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Centro de Pesquisa e Desenvolvimento em Engenharia Eletrica]. E-mails: doriana@cpdee.ufmg.br; selenios@eee.ufmg.br; aymore@cpdee.ufmg.br; Martinez, Carlos Barreira [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Dept. de Engenharia Hidraulica e Recursos Hidricos]. E-mail: martinez@cce.ufmg.br

    2000-07-01

    This work presents a comparative study among the direct solar energy utilization options ,through solar panels, coupled to the frequency inverter. These system store energy through batteries or through a reversible and hybrid solar/hydraulic system, coupled to a rectifier and a frequency inverter. There are two basic configurations for the systems being the first one composed of solar panels linked to a battery system , delivering electric energy through a three phase inverter. The second one is composed of solar panels connected to a small battery system and to a water reservoir that has the goal of operating as a reversible system during at night, or during load peak periods. In this ,it is presented a methodology for the designing and economic analysis, comparing this hybrid alternative, to the inverter plus batteries options. This methodology to the correct Energy Conversion System,which is economically advantageous due to the availability of the region. At the end, it is presented a 'case study' where viability of use , for the hybrid solar/hydraulic system in an isolated area, is verified. (author)

  8. Pre-Discovery Detection of ASASSN-18fv by Evryscope

    Science.gov (United States)

    Corbett, H.; Law, N.; Goeke, E.; Ratzloff, J.; Howard, W.; Fors, O.; del Ser, D.; Quimby, R. M.

    2018-03-01

    We have identified pre-discovery imaging of the probable classical nova ASASSN-18fv by Evryscope-South (http://evryscope.astro.unc.edu/), an array of 6-cm telescopes continuously monitoring 8000 square degrees of sky at 2-minute cadence from CTIO, Chile.

  9. Fluorescent lighting system of modified fast starting system; Sistema de alumbrado fluorescente de arranque rapido modificado

    Energy Technology Data Exchange (ETDEWEB)

    Mendoza E, Ernesto J [Manufacturera De Reactores, S. A., Mexico, D. F. (Mexico)

    1994-12-31

    This paper puts into perspective the possible energy saving in using hybrid ballasts for the operation of fast starting lamps. It presents in summarized form some fundamentals of the fluorescent lamps operation, as well as the fast starting modified systems. Also the results of field operation are shown, as well as the effects on the useful life of the lamp T12 as well as the T8. [Espanol] Este trabajo pone en perspectiva el ahorro de energia posible, al emplear balastros hibridos para operar lamparas de arranque rapido. Presenta en forma resumida algunos fundamentos de la operacion de lamparas fluorescentes, asi como de los sistemas de arranque rapido modificado. Se muestran tambien resultados de operacion de campo, asi como los efectos sobre la vida util de las lamparas, tanto T12 como T8.

  10. Fluorescent lighting system of modified fast starting system; Sistema de alumbrado fluorescente de arranque rapido modificado

    Energy Technology Data Exchange (ETDEWEB)

    Mendoza E, Ernesto J. [Manufacturera De Reactores, S. A., Mexico, D. F. (Mexico)

    1993-12-31

    This paper puts into perspective the possible energy saving in using hybrid ballasts for the operation of fast starting lamps. It presents in summarized form some fundamentals of the fluorescent lamps operation, as well as the fast starting modified systems. Also the results of field operation are shown, as well as the effects on the useful life of the lamp T12 as well as the T8. [Espanol] Este trabajo pone en perspectiva el ahorro de energia posible, al emplear balastros hibridos para operar lamparas de arranque rapido. Presenta en forma resumida algunos fundamentos de la operacion de lamparas fluorescentes, asi como de los sistemas de arranque rapido modificado. Se muestran tambien resultados de operacion de campo, asi como los efectos sobre la vida util de las lamparas, tanto T12 como T8.

  11. Antibody engineering using phage display with a coiled-coil heterodimeric Fv antibody fragment.

    Directory of Open Access Journals (Sweden)

    Xinwei Wang

    Full Text Available A Fab-like antibody binding unit, ccFv, in which a pair of heterodimeric coiled-coil domains was fused to V(H and V(L for Fv stabilization, was constructed for an anti-VEGF antibody. The anti-VEGF ccFv showed the same binding affinity as scFv but significantly improved stability and phage display level. Furthermore, phage display libraries in the ccFv format were constructed for humanization and affinity maturation of the anti-VEGF antibody. A panel of V(H frameworks and V(H-CDR3 variants, with a significant improvement in affinity and expressibility in both E. coli and yeast systems, was isolated from the ccFv phage libraries. These results demonstrate the potential application of the ccFv antibody format in antibody engineering.

  12. INCREASING OF THE EXPRESSION OF RECOMBINANT scFv-ANTIBODIES EFFICIENCY

    Directory of Open Access Journals (Sweden)

    O.V. Galkin

    2017-10-01

    Full Text Available Obtaining single-chain variable fragments (scFv of recombinant antibodies in E. coli cells is often associated with numerous problems causing low yields or inactive conformation of the product. The aim of this work was to study the influence of staphylococcal protein A fragment fused with scFv antibodies (SpA-tag on the efficiency of expression of final product. Examination of scFv antibodies of different origin and specificity has shown that in similar expression systems fused scFv is synthesized in much higher quantities than free scFv. Furthermore, the scFv antibodies in fused form retained their antigen-binding properties and the SpA fragment the ability to bind other immunoglobulins. Thus, the proposed strategy can be considered effective in improving the efficiency of scFv-antibodies production in E. coli cells.

  13. PET Imaging of 64Cu-DOTA-scFv-Anti-PSMA Lipid Nanoparticles (LNPs): Enhanced Tumor Targeting over Anti-PSMA scFv or Untargeted LNPs

    Science.gov (United States)

    Wong, Patty; Li, Lin; Chea, Junie; Delgado, Melissa K.; Crow, Desiree; Poku, Erasmus; Szpikowska, Barbara; Bowles, Nicole; Channappa, Divya; Colcher, David; Wong, Jeffrey Y.C.; Shively, John E.; Yazaki, Paul J.

    2017-01-01

    Introduction Single chain (scFv) antibodies are ideal targeting ligands due to their modular structure, high antigen specificity and affinity. These monovalent ligands display rapid tumor targeting but have limitations due to their fast urinary clearance. Methods An anti-prostate membrane antigen (PSMA) scFv with a site-specific cysteine was expressed and evaluated in a prostate cancer xenograft model by Cu-64 PET imaging. To enhance tumor accumulation, the scFv-cys was conjugated to the co-polymer DSPE-PEG-maleimide that spontaneously assembled into a homogeneous multivalent lipid nanoparticle (LNP). Results The targeted LNP exhibited a 2-fold increase in tumor uptake compared to the scFv alone using two different thiol ester chemistries. The anti-PSMA scFv-LNP exhibited a 1.6 fold increase in tumor targeting over the untargeted LNP. Conclusions The targeted anti-PSMA scFv-LNP showed enhanced tumor accumulation over the scFv alone or the untargeted DOTA-micelle providing evidence for the development of this system for drug delivery. Advances in Knowledge and implications for patient care Anti-tumor scFv antibody fragments have not achieved their therapeutic potential due to their fast blood clearance. Conjugation to a LNP enables multivalency to the tumor antigen as well as increased molecular size for chemotherapy drug delivery. PMID:28126683

  14. PET imaging of 64Cu-DOTA-scFv-anti-PSMA lipid nanoparticles (LNPs): Enhanced tumor targeting over anti-PSMA scFv or untargeted LNPs

    International Nuclear Information System (INIS)

    Wong, Patty; Li, Lin; Chea, Junie; Delgado, Melissa K.; Crow, Desiree; Poku, Erasmus; Szpikowska, Barbara; Bowles, Nicole; Channappa, Divya; Colcher, David; Wong, Jeffrey Y.C.; Shively, John E.; Yazaki, Paul J.

    2017-01-01

    Introduction: Single chain (scFv) antibodies are ideal targeting ligands due to their modular structure, high antigen specificity and affinity. These monovalent ligands display rapid tumor targeting but have limitations due to their fast urinary clearance. Methods: An anti-prostate membrane antigen (PSMA) scFv with a site-specific cysteine was expressed and evaluated in a prostate cancer xenograft model by Cu-64 PET imaging. To enhance tumor accumulation, the scFv-cys was conjugated to the co-polymer DSPE-PEG-maleimide that spontaneously assembled into a homogeneous multivalent lipid nanoparticle (LNP). Results: The targeted LNP exhibited a 2-fold increase in tumor uptake compared to the scFv alone using two different thiol ester chemistries. The anti-PSMA scFv-LNP exhibited a 1.6 fold increase in tumor targeting over the untargeted LNP. Conclusions: The targeted anti-PSMA scFv-LNP showed enhanced tumor accumulation over the scFv alone or the untargeted DOTA-micelle providing evidence for the development of this system for drug delivery. Advances in knowledge and implications for patient care: Anti-tumor scFv antibody fragments have not achieved their therapeutic potential due to their fast blood clearance. Conjugation to an LNP enables multivalency to the tumor antigen as well as increased molecular size for chemotherapy drug delivery.

  15. Implantação de pastagens sob sistema monocultivo e integrado com lavoura utilizando biofertilizante de cama de aviário como adubação de cobertura

    OpenAIRE

    SILVEIRA JUNIOR,Otacilio; SANTOS,Antônio Clementino dos; ROCHA,José Mario Lopes; FERREIRA,Caio Leonardo Silva; OLIVEIRA,Leonardo Bernardes Taverny de; RODRIGUES,Marcos Odilon Dias; RODRIGUES,Márcio Odilon Dias

    2015-01-01

    ResumoObjetivou-se avaliar o efeito da utilização de biofertilizante obtido através da biodigestão anaeróbica da cama de aviário nas características produtivas do capim Piatã, e nas características químicas do solo. Avaliou-se cinco doses de biofertilizantes (0; 10; 20; 30; 40m3.ha-1) em dois sistemas de cultivos (sistema monocultivo de capim Piatã e sistema consorciado de capim Piatã com sorgo forrageiro hibrido BRS 610). Foram verificadas as características agronômicas e estruturais submeti...

  16. Hybrid solar-electric oven construction prototype; Construccion de prototipo de horno hibrido solar-electrico

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez Roman, M. A; Pineda Pinon, J; Arcos Pichardo, A [CICATA, Santiago de Queretaro, Queretaro (Mexico)

    2013-03-15

    The oven construction consists of a solar collector system of cylindrical parabolic type, a heating through electrical resistance and a curing chamber. The warm fluid is air, which is injected into the chamber through forced draft. The temperature required in the system is within a range of 150 to 300 degrees Celsius. [Spanish] La construccion del horno consta de un sistema de captacion solar del tipo cilindrico parabolico, un sistema de calentamiento a traves de resistencias electricas y una camara de curado. El fluido a calentar es aire, el cual es inyectado dentro de la camara a traves de tiro forzado. La temperatura solicitada en el sistema es dentro de un rango de 150 a 300 grados centigrados.

  17. Toward low-cost affinity reagents: lyophilized yeast-scFv probes specific for pathogen antigens.

    Directory of Open Access Journals (Sweden)

    Sean A Gray

    Full Text Available The generation of affinity reagents, usually monoclonal antibodies, remains a critical bottleneck in biomedical research and diagnostic test development. Recombinant antibody-like proteins such as scFv have yet to replace traditional monoclonal antibodies in antigen detection applications, in large part because of poor performance of scFv in solution. To address this limitation, we have developed assays that use whole yeast cells expressing scFv on their surfaces (yeast-scFv in place of soluble purified scFv or traditional monoclonal antibodies. In this study, a nonimmune library of human scFv displayed on the surfaces of yeast cells was screened for clones that bind to recombinant cyst proteins of Entamoeba histolytica, an enteric pathogen of humans. Selected yeast-scFv clones were stabilized by lyophilization and used in detection assay formats in which the yeast-scFv served as solid support-bound monoclonal antibodies. Specific binding of antigen to the yeast-scFv was detected by staining with rabbit polyclonal antibodies. In flow cytometry-based assays, lyophilized yeast-scFv reagents retained full binding activity and specificity for their cognate antigens after 4 weeks of storage at room temperature in the absence of desiccants or stabilizers. Because flow cytometry is not available to all potential assay users, an immunofluorescence assay was also developed that detects antigen with similar sensitivity and specificity. Antigen-specific whole-cell yeast-scFv reagents can be selected from nonimmune libraries in 2-3 weeks, produced in vast quantities, and packaged in lyophilized form for extended shelf life. Lyophilized yeast-scFv show promise as low cost, renewable alternatives to monoclonal antibodies for diagnosis and research.

  18. Baculovirus display of single chain antibody (scFv using a novel signal peptide

    Directory of Open Access Journals (Sweden)

    Gonzalez Gaëlle

    2010-11-01

    Full Text Available Abstract Background Cells permissive to virus can become refractory to viral replication upon intracellular expression of single chain fragment variable (scFv antibodies directed towards viral structural or regulatory proteins, or virus-coded enzymes. For example, an intrabody derived from MH-SVM33, a monoclonal antibody against a conserved C-terminal epitope of the HIV-1 matrix protein (MAp17, was found to exert an inhibitory effect on HIV-1 replication. Results Two versions of MH-SVM33-derived scFv were constructed in recombinant baculoviruses (BVs and expressed in BV-infected Sf9 cells, N-myristoylation-competent scFvG2/p17 and N-myristoylation-incompetent scFvE2/p17 protein, both carrying a C-terminal HA tag. ScFvG2/p17 expression resulted in an insoluble, membrane-associated protein, whereas scFvE2/p17 was recovered in both soluble and membrane-incorporated forms. When coexpressed with the HIV-1 Pr55Gag precursor, scFvG2/p17 and scFvE2/p17 did not show any detectable negative effect on virus-like particle (VLP assembly and egress, and both failed to be encapsidated in VLP. However, soluble scFvE2/p17 isolated from Sf9 cell lysates was capable of binding to its specific antigen, in the form of a synthetic p17 peptide or as Gag polyprotein-embedded epitope. Significant amounts of scFvE2/p17 were released in the extracellular medium of BV-infected cells in high-molecular weight, pelletable form. This particulate form corresponded to BV particles displaying scFvE2/p17 molecules, inserted into the BV envelope via the scFv N-terminal region. The BV-displayed scFvE2/p17 molecules were found to be immunologically functional, as they reacted with the C-terminal epitope of MAp17. Fusion of the N-terminal 18 amino acid residues from the scFvE2/p17 sequence (N18E2 to another scFv recognizing CD147 (scFv-M6-1B9 conferred the property of BV-display to the resulting chimeric scFv-N18E2/M6. Conclusion Expression of scFvE2/p17 in insect cells using a BV

  19. Sistema Solar

    OpenAIRE

    Federación de Asociaciones de Astronomía Cielo de Comellas

    2011-01-01

    Lección sobre el Sistema Solar. Curso de Astronomía Básica, segunda edición, impartido por los miembros de la Federación de Asociaciones de Astronomía Cielo de Comellas. Casa de la Ciencia, sábados, del 24 de septiembre al 22 de octubre de 2011

  20. DESARROLLO DE UN SISTEMA DE CONTROL PARA LA CAPTURA Y MEDICIÓN EXPERIMENTAL DE LA EFICIENCIA Y CURVA CARACTERÍSTICA I-V EN TIEMPO REAL DE UN SISTEMA FOTOVOLTAICO UTILIZANDO LABVIEW® Y ARDUINO

    Directory of Open Access Journals (Sweden)

    Juan Pablo Vargas Bautista

    2015-07-01

    Full Text Available En el presente artículo se presenta un sistema de control para la visualización en tiempo real de datos experimentales de la curva de eficiencia y de la curva característica I-V (corriente versus voltaje de un panel fotovoltaico (FV SUNTECH-STP030-12. El sistema está compuesto de un programa desarrollado en LabView® y un circuito electrónico de captura y procesamiento de datos.  Se utilizó un microprocesador Arduino UNO R3 para la interface entre los datos del panel FV y el programa en LabView®.  Los resultados muestran que es posible utilizar microprocesadores de bajo costo con suficiente capacidad de procesamiento en tiempo real de la información obtenida de los paneles FV.

  1. Evolution of the retroviral restriction gene Fv1: inhibition of non-MLV retroviruses.

    Directory of Open Access Journals (Sweden)

    Melvyn W Yap

    2014-03-01

    Full Text Available Fv1 is the prototypic restriction factor that protects against infection by the murine leukemia virus (MLV. It was first identified in cells that were derived from laboratory mice and was found to be homologous to the gag gene of an endogenous retrovirus (ERV. To understand the evolution of the host restriction gene from its retroviral origins, Fv1s from wild mice were isolated and characterized. Most of these possess intact open reading frames but not all restricted N-, B-, NR-or NB-tropic MLVs, suggesting that other viruses could have played a role in the selection of the gene. The Fv1s from Mus spretus and Mus caroli were found to restrict equine infectious anemia virus (EIAV and feline foamy virus (FFV respectively, indicating that Fv1 could have a broader target range than previously thought, including activity against lentiviruses and spumaviruses. Analyses of the Fv1 sequences revealed a number of residues in the C-terminal region that had evolved under positive selection. Four of these selected residues were found to be involved in the novel restriction by mapping studies. These results strengthen the similarities between the two capsid binding restriction factors, Fv1 and TRIM5α, which support the hypothesis that Fv1 defended mice against waves of retroviral infection possibly including non-MLVs as well as MLVs.

  2. A novel fusion protein of IP10-scFv retains antibody specificity and chemokine function

    Energy Technology Data Exchange (ETDEWEB)

    Junqing, Guo; Liu, Chen; Hongwu, Ai; Jiannian, Jing; Jiyong, Zhou; Chuyu, Zhang; Shangyou, You

    2004-07-23

    We combined the specificity of tumor-specific antibody with the chemokine function of interferon-{gamma} inducible protein 10 (IP-10) to recruit immune effector cells in the vicinity of tumor cells. A novel fusion protein of IP10-scFv was constructed by fusing mouse IP-10 to V{sub H} region of single-chain Fv fragment (scFv) against acidic isoferritin (AIF), and expressed in NS0 murine myeloma cells. The IP10-scFv fusion protein was shown to maintain the specificity of the antiAIF scFv with similar affinity constant, and bind to the human hepatocarcinoma SMMC 7721 cells secreting AIF as well as the activated mouse T lymphocytes expressing CXCR3 receptor. Furthermore, the IP10-scFv protein either in solution or bound on the surface of SMMC 7721 cells induced significant chemotaxis of mouse T cells in vitro. The results indicate that the IP10-scFv fusion protein possesses both bioactivities of the tumor-specific antibody and IP-10 chemokine, suggesting its possibility to induce an enhanced immune response against the residual tumor cells in vivo.

  3. A novel fusion protein of IP10-scFv retains antibody specificity and chemokine function

    International Nuclear Information System (INIS)

    Guo Junqing; Chen Liu; Ai Hongwu; Jing Jiannian; Zhou Jiyong; Zhang Chuyu; You Shangyou

    2004-01-01

    We combined the specificity of tumor-specific antibody with the chemokine function of interferon-γ inducible protein 10 (IP-10) to recruit immune effector cells in the vicinity of tumor cells. A novel fusion protein of IP10-scFv was constructed by fusing mouse IP-10 to V H region of single-chain Fv fragment (scFv) against acidic isoferritin (AIF), and expressed in NS0 murine myeloma cells. The IP10-scFv fusion protein was shown to maintain the specificity of the antiAIF scFv with similar affinity constant, and bind to the human hepatocarcinoma SMMC 7721 cells secreting AIF as well as the activated mouse T lymphocytes expressing CXCR3 receptor. Furthermore, the IP10-scFv protein either in solution or bound on the surface of SMMC 7721 cells induced significant chemotaxis of mouse T cells in vitro. The results indicate that the IP10-scFv fusion protein possesses both bioactivities of the tumor-specific antibody and IP-10 chemokine, suggesting its possibility to induce an enhanced immune response against the residual tumor cells in vivo

  4. EXPERIMENTAL CHALLENGE STUDY OF FV3-LIKE RANAVIRUS INFECTION IN PREVIOUSLY FV3-LIKE RANAVIRUS INFECTED EASTERN BOX TURTLES (TERRAPENE CAROLINA CAROLINA) TO ASSESS INFECTION AND SURVIVAL.

    Science.gov (United States)

    Hausmann, Jennifer C; Wack, Allison N; Allender, Matthew C; Cranfield, Mike R; Murphy, Kevin J; Barrett, Kevin; Romero, Jennell L; Wellehan, James F X; Blum, Stella A; Zink, M Christine; Bronson, Ellen

    2015-12-01

    The Maryland Zoo in Baltimore experienced an outbreak of Frog virus-3 (FV3)-like ranavirus during the summer of 2011, during which 14 of 27 (52%) of its captive eastern box turtles (Terrapene carolina carolina) survived. To assess survival, immunity, and viral shedding, an experimental challenge study was performed in which the surviving, previously infected turtles were reinfected with the outbreak strain of FV3-like ranavirus. Seven turtles were inoculated with virus intramuscularly and four control turtles received saline intramuscularly. The turtles were monitored for 8 wk with blood and oral swabs collected for quantitative polymerase chain reaction (qPCR). During that time, one of seven (14%) inoculated turtles and none of the controls (0%) died; there was no significant difference in survival. Clinical signs of the inoculated turtles, except for the turtle that died, were mild compared to the original outbreak. Quantitative PCR for FV3-like ranavirus on blood and oral swabs was positive for all inoculated turtles and negative for all controls. The turtle that died had intracytoplasmic inclusion bodies in multiple organs. Three inoculated and two control turtles were euthanized at the end of the study. No inclusion bodies were present in any of the organs. Quantitative PCR detected FV3-like ranavirus in the spleen of a control turtle, which suggested persistence of the virus. The surviving five turtles were qPCR-negative for FV3-like ranavirus from blood and oral swabs after brumation. Quantitative PCR for Terrapene herpesvirus 1 found no association between ranavirus infection and herpesvirus loads. In conclusion, previously infected eastern box turtles can be reinfected with the same strain of FV3-like ranavirus and show mild to no clinical signs but can shed the virus from the oral cavity.

  5. AFSC/ABL: Origins of salmon seized from the F/V Arctic Wind

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Samples of chum (Oncorhynchus keta), sockeye (O. nerka), and chinook salmon (O. tshawytscha) seized from the F/V Arctic Wind were analyzed to determine their region...

  6. FvVE1 Regulates Biosynthesis of Fumonisins and Fusarins in Fusarium verticillioides

    Science.gov (United States)

    MYUNG, KYUNG; LI, SHAOJIE; BUTCHKO, ROBERT A.E.; BUSMAN, MARK; PROCTOR, ROBERT H; ABBAS, HAMED K.; CALVO, ANA M.

    2009-01-01

    The veA gene positively regulates sterigmatocystin production in Aspergillus nidulans and aflatoxin production in A. parasiticus and A. flavus. Whether veA homologs have a role in regulating secondary metabolism in other fungal genera is unknown. In this study, we examined the role of the veA homolog, FvVE1, on production of two mycotoxin families, fumonisins and fusarins, in the important corn pathogen F. verticillioides. We found that FvVE1 deletion completely suppressed fumonisin production on two natural substrates, corn and rice. Furthermore, our results revealed that FvVE1 is necessary for the expression of the pathway-specific regulatory gene FUM21 and structural genes in the fumonisin biosynthetic gene (FUM) cluster. FvVE1 deletion also blocked production of fusarins. The effects of FvVE1 deletion on the production of these toxins were found to be the same in two separate mating types. Our results strongly suggest that FvVE1 play an important role in regulating mycotoxin production in F. verticillioides. PMID:19382792

  7. Xenopus-FV3 host-pathogen interactions and immune evasion.

    Science.gov (United States)

    Jacques, Robert; Edholm, Eva-Stina; Jazz, Sanchez; Odalys, Torres-Luquis; Francisco, De Jesús Andino

    2017-11-01

    We first review fundamental insights into anti-ranavirus immunity learned with the Xenopus laevis/ranavirus FV3 model that are generally applicable to ectothermic vertebrates. We then further investigate FV3 genes involved in immune evasion. Focusing on FV3 knockout (KO) mutants defective for a putative viral caspase activation and recruitment domain-containing (CARD)-like protein (Δ64R-FV3), a β-hydroxysteroid dehydrogenase homolog (Δ52L-FV3), and an immediate-early18kDa protein (FV3-Δ18K), we assessed the involvement of these viral genes in replication, dissemination and interaction with peritoneal macrophages in tadpole and adult frogs. Our results substantiate the role of 64R and 52L as critical immune evasion genes, promoting persistence and dissemination in the host by counteracting type III IFN in tadpoles and type I IFN in adult frogs. Comparably, the substantial accumulation of genome copy numbers and exacerbation of type I and III IFN gene expression responses but deficient release of infectious virus suggests that 18K is a viral regulatory gene. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. Methodology to optimize the cost of deployment of a wind-solar hybrid system; Metodologia para otimizar o custo da implantacao de um sistema hibrido eolico-solar

    Energy Technology Data Exchange (ETDEWEB)

    Nogueira, Jose Wilson Lage [Universidade Federal do Rio Grande Norte (UERN), Natal, RN (Brazil). Dept. de Engenharia Mecanica], e-mail: wilson@ufrnet.br; Rocha, Brismark Goes da [Universidade do Estado do Rio Grande do Norte (UERN), Patu, RN (Brazil). Dept. de Matematica], e-mail: brismarkrocha@uern.br

    2008-07-01

    Purposes the application of a methodology to optimize the implantation cost of an wind-solar hybrid system, to potencies between 2.250 W and 3.750 W. The developed mathematical model was obtained through the Multiple Linear Regression technique, on the basis of the previous knowledge of variables: necessary capacity of storage, total daily energy demand, wind power, module power and module number. These variables are gotten by means of sizing. Parametric statistical: T-student tests had been used to detect the significant difference in the average of total cost to being considered the diameter of the wind. Parametric statistical T-student tests had been used to detect the significant difference in the average of total cost to being considered the diameter of the wind, F-Snedecor in the variance analysis to test if the coefficients of the considered model are significantly different of zero and test not-parametric statistical by Friedman, to verify if there is difference in the total cost, by being considered the photovoltaic module powers. In decision of hypothesis tests was considered a 5%-significant level. The configurations module powers showed significant differences in total cost of investment by considering an electrical motor of 3 HP. The configurations module powers showed significant differences in total cost of investment by considering an electrical motor of 5 HP only to wind speed of 4 m/s and 6 m/s in wind of 3 m, 4 m and 5 m of diameter. There was not significant difference in costs to diameters of winds of 3 m and 4 m. A computational program was developed to assist the study of several configurations that optimizes the implantation cost of an wind-solar through considered mathematical model. (author)

  9. Optimization of ground source heat pumps systems through the implementation of hybrid systems; Optimizacion de las instalaciones de bomba de calor geotermica mediante la implementacion de sistemas hibridos

    Energy Technology Data Exchange (ETDEWEB)

    Magraner, T.; Quilis, M. S.; Martinez, S.

    2009-07-01

    One of the fundamental aspects to consider in the design of a ground heat pump system is the heat balance in the ground since thermal saturation of the ground produces a decreasing of the performance of the system throughout its useful life. The hybrid geothermal system which combined geothermal heat pump system with other systems for generation or dissipation energy are very suitable for balancing the heat exchanger meters needed for the proper functioning of the system, important aspect to reduce costs and achieve attractive return periods of the initial investment. Energesis Ingenieria has developed and implemented in two office buildings, a design of hybrid systems based on the combination of a geothermal heat pump and air-condensed units (dry coolers) that can ensure energy efficiencies comparable to geothermal pure systems, reducing substantially the investment cost. (Author) 5 refs.

  10. Comparative evaluation of hybrid systems of natural gas cogeneration and sugar cane bagasse; Avaliacao comparativa de sistemas hibridos de cogeracao a gas natutral e bagaco de cana

    Energy Technology Data Exchange (ETDEWEB)

    Zamboni, Leonardo Moneci; Tribess, Arlindo [Sao Paulo Univ., SP (Brazil). Escola Politecnica. Dept. de Engenharia Mecanica]. E-mail: leonardo.zamboni@poli.usp.br; atribess@usp.br

    2006-07-01

    The consumption of electricity in Brazil and mainly in the State of Sao Paulo is increasing gradually. On the other hand, the hydraulic potential is practically exhausted and the government has no resources for such new investments. One solution is the construction of thermo electrical plants with the use of the natural gas and sugar cane bagasse. The natural gas has the advantage of being available in great amount and less pollutant. And the sugar cane bagasse, besides being a by-product of low value, does not cause a global pollution. The work consists of the determination of the best option considering criterion of minimum cost for kWh of energy produced. For such, thermo economic analysis with electricity and steam production costs evaluation in exergetic basis, was accomplished. In the evaluations the consumption of natural gas and the costs of the sugar cane bagasse were varied. The results show that the cogeneration plant with combined cycle using natural gas and burning sugar cane bagasse in the recovery boiler presents the smallest cost of electricity and steam generation (even not being the cycle with larger exergetic efficiency). On the other hand, for a natural gas cost of 140 US$/t and a cost of sugar cane bagasse superior to 10,50 US$/t the cogeneration plant with combined cycle using only natural gas (and, therefore not burning or gasifying sugar cane bagasse) presented the smallest cost of electricity and steam generation. (author)

  11. Multicriteria analysis of the hybrid systems with biogas: fuzzy set and rules; Analise multicriterio de sistemas hibridos com biogas: conjuntos e regras fuzzy

    Energy Technology Data Exchange (ETDEWEB)

    Barin, A.; Canha, L.; Abaide, A.; Magnago, K. [Federal University of Santa Maria (UFSM), RS (Brazil)], E-mail: chbarin@gmail.com; Machado, R. [Universidade de Sao Paulo (EESC/USP), Sao Carlos, SP (Brazil). Escola de Engenharia], E-mail: rquadros@sel.eesc.usp.br

    2009-07-01

    A multicriteria analysis to manage de renewable sources of energy is presented, identifying the most appropriate hybrid system to be used as distributed generation of electric energy using biogas. In this methodology, fuzzy sets and rules are defined simulated in the software MATLAB, where the main characteristics of the operation and application of hybrid systems of electric power generation are considered. The main generation system, that can use the biogas, as micro turbines and fuel cells, are evaluated. Afterwards, the systems of energy storage are analyzed: flywheel, H{sub 2} storage and conventional and redox batteries. For the development of the proposed methodology, it was considered the following criteria: efficiency, costs, technological maturity, environmental impacts, the amplitude of the system action (power range), useful life, co-generation possibility and operation temperature. A classification, by priority order, for the use of the sources and storages associated to the environment and cost scenarios is also presented.

  12. Generation of electric power through wind-diesel hybrid system for a hospital; Geracao de energia eletrica atraves de sistema hibrido diesel-eolico para um hospital

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Silvio Carlos Anibal de; Freire, Raphael Lopes [Universidade Federal do Rio de Janeiro (DEM/UFRJ), RJ (Brazil). Dept. de Engenharia Mecanica], e-mail: silvioa@gmail.com, e-mail: raphaellfreire@gmail.com

    2008-07-01

    This paper presents a wind-diesel hybrid power simulation using the software Homer. The model is applied to the case study of Hospital das Clinicas da UNICAMP Analysis of several alternative energy facilities like wind, photovoltaic (PV), and connection of the isolated system with the grid is done. The costs used in the simulation indicated that the best results were obtained with the wind-diesel system. The payback period for the investment in the system is 8 years. (author)

  13. Diseño de multiplexores hibridos para sistemas de comunicaciones ópticas mediante la concatenación de dispositivos selectivos en longitud de onda

    OpenAIRE

    Rodríguez Horche, Paloma; López-Amo Sainz, Manuel; Muriel Fernández, Miguel Ángel; Martín Pereda, José Antonio

    1987-01-01

    La creciente demandada de información por parte del abonado hace necesaria la inclusión de nuevos servicios como la transmisión da datos de alta y baja velocidad, señales de vídeo, etc. La única solución que promete viabilidad es la que puede desarrollarse con Fibra Óptica.

  14. Hybrid solar-PLG system for industrial scale steam and hot water generation; Sistema hibrido solar-GLP para geracao de vapor e agua quente em escala industrial

    Energy Technology Data Exchange (ETDEWEB)

    Saidel, Marco A.; Monteiro, Marcio D.; Gimenes, Andre L.V.; Fujii, Ricardo J. [Universidade de Sao Paulo (GEPEA/EPUSP), SP (Brazil). Dept. Engenharia Energia e Automacao Eletricas. Grupo de Energia], e-mail: saidel@pea.usp.br, e-mail: marcio.monteiro@poli.usp.br, e-mail: gimenes@gmail.com, e-mail: fujii@gmail.com

    2008-07-01

    This paper presents an initiative conceived for attending to objectives of the PUREFA (Program for Rational Use of Energy and Alternative Sources) of the Sao Paulo university, Brazil. The indicative consists of the implantation of a solar collector system for pre-heating of the water used in the production of the steam consumed at the university restaurant, with a production of 5800 meals per day. This system (auxiliary to the original steam boiler) pre-heats the water of the boiler minimizing the energy expenses for the production of steam and hot water.

  15. Fast conversion of scFv to Fab antibodies using type IIs restriction enzymes.

    Science.gov (United States)

    Sanmark, Hanna; Huovinen, Tuomas; Matikka, Tero; Pettersson, Tiina; Lahti, Maria; Lamminmäki, Urpo

    2015-11-01

    Single chain variable fragment (scFv) antibody libraries are widely used for developing novel bioaffinity reagents, although Fab or IgG molecules are the preferred antibody formats in many final applications. Therefore, rapid conversion methods for combining multiple DNA fragments are needed to attach constant domains to the scFv derived variable domains. In this study we describe a fast and easy cloning method for the conversion of single framework scFv fragments to Fab fragments using type IIS restriction enzymes. All cloning steps excluding plating of the Fab transformants can be done in 96 well plates and the procedure can be completed in one working day. The concept was tested by converting 69 scFv clones into Fab format on 96 well plates, which resulted in 93% success rate. The method is particularly useful as a high-throughput tool for the conversion of the chosen scFv clones into Fab molecules in order to analyze them as early as possible, as the conversion can significantly affect the binding properties of the chosen clones. Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Detection of Metallothionein in Javanese Medaka (Oryzias javanicus, Using a scFv-Immobilized Protein Chip

    Directory of Open Access Journals (Sweden)

    Euiyeon Lee

    2018-04-01

    Full Text Available Environmental pollution by various industrial chemicals and biological agents poses serious risks to human health. Especially, marine contamination by potentially toxic elements (PTEs has become a global concern in recent years. Many efforts have been undertaken to monitor the PTE contamination of the aquatic environment. However, there are few approaches available to assess the PTE exposure of aquatic organisms. In this research, we developed a strategy to evaluate the heavy metal exposure of marine organisms, by measuring the expression levels of metallothionein protein derived from Oryzias javanicus (OjaMT. OjaMT is a biomarker of heavy metal exposure because the expression level increases upon heavy metal exposure. The developed assay is based on a real-time, label-free surface plasmon resonance (SPR measurement. Anti-OjaMT antibody and anti-OjaMT single-chain fragment of variable region (scFv were used as detection probes. Two types of SPR sensor chips were fabricated, by immobilizing antibody or Cys3-tagged scFv (scFv-Cys3 in a controlled orientation and were tested for in situ label-free OjaMT detection. Compared to the antibody-presenting sensor chips, the scFv-presenting sensor chips showed improved performance, displaying enhanced sensitivity and enabling semi-quantitative detection. The portable SPR system combined with scFv-immobilized sensor chips is expected to provide an excellent point-of-care testing system that can monitor target biomarkers in real time.

  17. Improved Soluble ScFv ELISA Screening Approach for Antibody Discovery Using Phage Display Technology.

    Science.gov (United States)

    Tohidkia, Mohammad R; Sepehri, Maryam; Khajeh, Shirin; Barar, Jaleh; Omidi, Yadollah

    2017-09-01

    Phage display technology (PDT) is a powerful tool for the isolation of recombinant antibody (Ab) fragments. Using PDT, target molecule-specific phage-Ab clones are enriched through the "biopanning" process. The individual specific binders are screened by the monoclonal scFv enzyme-linked immunosorbent assay (ELISA) that may associate with inevitable false-negative results. Thus, in this study, three strategies were investigated for optimization of the scFvs screening using Tomlinson I and J libraries, including (1) optimizing the expression of functional scFvs, (2) improving the sensitivity of ELISA, and (3) preparing different samples containing scFvs. The expression of all scFv Abs was significantly enhanced when scFv clones were cultivated in the terrific broth (TB) medium at the optimum temperature of 30 °C. The protein A-conjugated with horseradish peroxidase (HRP) was found to be a well-suited reagent for the detection of Ag-bound scFvs in comparison with either anti-c-myc Ab or the mixing procedure. Based on our findings, it seems there is no universal media supplement for an improved expression of all scFvs derived from both Tomlinson I and J libraries. We thus propose that expression of scFv fragments in a microplate scale is largely dependent on a variety of parameters, in particular the scFv clones and relevant sequences.

  18. Good Practices for Learning to Recognize Actions Using FV and VLAD.

    Science.gov (United States)

    Wu, Jianxin; Zhang, Yu; Lin, Weiyao

    2016-12-01

    High dimensional representations such as Fisher vectors (FV) and vectors of locally aggregated descriptors (VLAD) have shown state-of-the-art accuracy for action recognition in videos. The high dimensionality, on the other hand, also causes computational difficulties when scaling up to large-scale video data. This paper makes three lines of contributions to learning to recognize actions using high dimensional representations. First, we reviewed several existing techniques that improve upon FV or VLAD in image classification, and performed extensive empirical evaluations to assess their applicability for action recognition. Our analyses of these empirical results show that normality and bimodality are essential to achieve high accuracy. Second, we proposed a new pooling strategy for VLAD and three simple, efficient, and effective transformations for both FV and VLAD. Both proposed methods have shown higher accuracy than the original FV/VLAD method in extensive evaluations. Third, we proposed and evaluated new feature selection and compression methods for the FV and VLAD representations. This strategy uses only 4% of the storage of the original representation, but achieves comparable or even higher accuracy. Based on these contributions, we recommend a set of good practices for action recognition in videos for practitioners in this field.

  19. Isolation and characterization of anti c-met single chain fragment variable (scFv) antibodies.

    Science.gov (United States)

    Qamsari, Elmira Safaie; Sharifzadeh, Zahra; Bagheri, Salman; Riazi-Rad, Farhad; Younesi, Vahid; Abolhassani, Mohsen; Ghaderi, Sepideh Safaei; Baradaran, Behzad; Somi, Mohammad Hossein; Yousefi, Mehdi

    2017-12-01

    The receptor tyrosine kinase (RTK) Met is the cell surface receptor for hepatocyte growth factor (HGF) involved in invasive growth programs during embryogenesis and tumorgenesis. There is compelling evidence suggesting important roles for c-Met in colorectal cancer proliferation, migration, invasion, angiogenesis, and survival. Hence, a molecular inhibitor of an extracellular domain of c-Met receptor that blocks c-Met-cell surface interactions could be of great thera-peutic importance. In an attempt to develop molecular inhibitors of c-Met, single chain variable fragment (scFv) phage display libraries Tomlinson I + J against a specific synthetic oligopeptide from the extracellular domain of c-Met receptor were screened; selected scFv were then characterized using various immune techniques. Three c-Met specific scFv (ES1, ES2, and ES3) were selected following five rounds of panning procedures. The scFv showed specific binding to c-Met receptor, and significantly inhibited proliferation responses of a human colorectal carcinoma cell line (HCT-116). Moreover, anti- apoptotic effects of selected scFv antibodies on the HCT-116 cell line were also evaluated using Annexin V/PI assays. The results demonstrated rates of apoptotic cell death of 46.0, 25.5, and 37.8% among these cells were induced by use of ES1, ES2, and ES3, respectively. The results demonstrated ability to successfully isolate/char-acterize specific c-Met scFv that could ultimately have a great therapeutic potential in immuno-therapies against (colorectal) cancers.

  20. Characterization of a Single Chain Fv Antibody that Reacts with Free Morphine

    OpenAIRE

    Matsukizono, Miho; Kamegawa, Mariko; Tanaka, Koichi; Kohra, Shinya; Arizono, Koji; Hamazoe, Yuta; Sugimura, Kazuhisa

    2013-01-01

    An immune phage library derived from mice, hyperimmunized with morphine-conjugated BSA, was used to isolate a single-chain Fv (scFv) clone, M86, with binding activity to morphine-conjugated thyroglobulin (morphine-C-Tg) but not to codeine-, cocaine-, or ketamine-conjugated Tg. Surface plasmon resonance analysis using a morphine-C-Tg-coupled CM5 sensor chip showed that the Kd value was 1.26 × 10−8 M. To analyze its binding activity to free morphine and related compounds, we performed a competi...

  1. A humanized anti-M2 scFv shows protective in vitro activity against influenza

    Energy Technology Data Exchange (ETDEWEB)

    Bradbury, Andrew M [Los Alamos National Laboratory; Velappan, Nileena [Los Alamos National Laboratory; Schmidt, Jurgen G [Los Alamos National Laboratory

    2008-01-01

    M2 is one of the most conserved influenza proteins, and has been widely prospected as a potential universal vaccine target, with protection predominantly mediated by antibodies. In this paper we describe the creation of a humanized single chain Fv from 14C2, a potent monoclonal antibody against M2. We show that the humanized scFv demonstrates similar activity to the parental mAb: it is able to recognize M2 in its native context on cell surfaces and is able to show protective in vitro activity against influenza, and so represents a potential lead antibody candidate for universal prophylactic or therapeutic intervention in influenza.

  2. Dynamics of Apis mellifera Filamentous Virus (AmFV) Infections in Honey Bees and Relationships with Other Parasites.

    Science.gov (United States)

    Hartmann, Ulrike; Forsgren, Eva; Charrière, Jean-Daniel; Neumann, Peter; Gauthier, Laurent

    2015-05-22

    Apis mellifera filamentous virus (AmFV) is a large double stranded DNA virus of honey bees, but its relationship with other parasites and prevalence are poorly known. We analyzed individual honey bees from three colonies at different times post emergence in order to monitor the dynamics of the AmFV gut colonization under natural conditions. Prevalence and loads of microsporidia and trypanosomes were also recorded, as well as five common honey bee RNA viruses. The results show that a high proportion of bees get infected with AmFV during the first week post-emergence (75%) and that AmFV DNA levels remained constant. A similar pattern was observed for microsporidia while trypanosomes seem to require more time to colonize the gut. No significant associations between these three infections were found, but significant positive correlations were observed between AmFV and RNA viruses. In parallel, the prevalence of AmFV in France and Sweden was assessed from pooled honey bee workers. The data indicate that AmFV is almost ubiquitous, and does not seem to follow seasonal patterns, although higher viral loads were significantly detected in spring. A high prevalence of AmFV was also found in winter bees, without obvious impact on overwintering of the colonies.

  3. Fv-clasp: An Artificially Designed Small Antibody Fragment with Improved Production Compatibility, Stability, and Crystallizability.

    Science.gov (United States)

    Arimori, Takao; Kitago, Yu; Umitsu, Masataka; Fujii, Yuki; Asaki, Ryoko; Tamura-Kawakami, Keiko; Takagi, Junichi

    2017-10-03

    Antibody fragments are frequently used as a "crystallization chaperone" to aid structural analysis of complex macromolecules that are otherwise crystallization resistant, but conventional fragment formats have not been designed for this particular application. By fusing an anti-parallel coiled-coil structure derived from the SARAH domain of human Mst1 kinase to the variable region of an antibody, we succeeded in creating a novel chimeric antibody fragment of ∼37 kDa, termed "Fv-clasp," which exhibits excellent crystallization compatibility while maintaining the binding ability of the original IgG molecule. The "clasp" and the engineered disulfide bond at the bottom of the Fv suppressed the internal mobility of the fragment and shielded hydrophobic residues, likely contributing to the high heat stability and the crystallizability of the Fv-clasp. Finally, Fv-clasp antibodies showed superior "chaperoning" activity over conventional Fab fragments, and facilitated the structure determination of an ectodomain fragment of integrin α6β1. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. First study of C2491T FV mutation with ischaemic stroke risk in ...

    Indian Academy of Sciences (India)

    Faculty of Medicine and Pharmacy, Department of Genetic and Molecular Pathology Laboratory (LGPM),. Tarek Ibn Ziad, QH, Hassan II University, 9154 Casablanca, Morocco. [Diakite B., Hamzi K., Hmimech W., Nadifi S. and GMRAVC 2015 First study of C2491T FV mutation with ischaemic stroke risk in Morocco. J. Genet.

  5. BRITE nanosatellite serendipitously captures oscillatory rise and fall of ASASSN-18fv

    Science.gov (United States)

    Kuschnig, R.; Pigulski, A.; Moffat, A. F. J.; Matthews, J. M.; Zwintz, K.; Baade, D.; Handler, G.; Weiss, W. W.; Wade, G. A.; Rucinski, S. M.; Pablo, H.; Koudelka, O.; Smolec, R.; Popowicz, A.; Neiner, C.; Daszynska-Daszkiewicz, J.; Lovekin, C.; St-Louis, N.; Pamyatnykh, A. A.; Rowe, J.; Orleanski, P.; Mochnacki, S.; Schwarzenberg-Czerny, A.

    2018-04-01

    One of the five satellites in BRITE-Constellation (http://www.brite-constellation.at/) was obtaining time-series optical photometry of the star HD 92063, only 2 arcmin from the 'Possible, Very Bright Galactic Nova ASASSN-18fv' reported on 21 March 2018 (2018-03-20.32) by ATel #11454.

  6. Suspension kinematic analysis of UTeM’s FV Malaysia electric vehicle racing car

    NARCIS (Netherlands)

    Abdul Manaf, M.Z.; Latif, M.F.A.; Razak, M.S.A.; Hassan, M.Z.B.; Rosley, M.I.F.

    2016-01-01

    The purpose of this paper is to investigate the kinematic performance of students’ racing car, namely UTeM’s FV Malaysia Electric Vehicle. An elasto-kinematic analysis approach is used to predict the car’s performance during straight line drive and curvature drive. Two suspension design factors

  7. FvSet2 regulates fungal growth, pathogenicity, and secondary metabolism in Fusarium verticillioides.

    Science.gov (United States)

    Gu, Qin; Wang, Zhenzhong; Sun, Xiao; Ji, Tiantian; Huang, Hai; Yang, Yang; Zhang, Hao; Tahir, Hafiz Abdul Samad; Wu, Liming; Wu, Huijun; Gao, Xuewen

    2017-10-01

    Histone H3 lysine 36 methylation (H3K36me) is generally associated with activation of gene expression in most eukaryotic cells. However, the function of H3K36me in filamentous fungi is largely unknown. Set2 is the sole lysine histone methyltransferase (KHMTase) enzyme responsible for the methylation of H3K36 in Saccharomyces cerevisiae. In the current study, we identified a single ortholog of S. cerevisiae Set2 in Fusarium verticillioides. We report that FvSet2 is responsible for the trimethylation of H3K36 (H3K36me3). The FvSET2 deletion mutant (ΔFvSet2) showed significant defects in vegetative growth, FB 1 biosynthesis, pigmentation, and fungal virulence. Furthermore, trimethylation of H3K36 was found to be important for active transcription of genes involved in FB 1 and bikaverin biosyntheses. These data indicate that FvSet2 plays an important role in the regulation of secondary metabolism, vegetative growth and fungal virulence in F. verticillioides. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. Atlantic mackerel and Horse mackerel egg survey 2016: Dutch participation on board FV Atlantic Lady: May

    NARCIS (Netherlands)

    Damme, van C.J.G.

    2016-01-01

    From 10 till 25 May 2016 IMARES carried out a mackerel and horse mackerel egg survey on board the FV Atlantic Lady. This survey was part of the international mackerel and horse mackerel egg survey coordinated by ICES. The Redersvereniging voor de Zeevisserij (RVZ) asked IMARES to carry out this

  9. Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands

    Directory of Open Access Journals (Sweden)

    Hardy Michele E

    2008-01-01

    Full Text Available Abstract Background Noroviruses cause epidemic outbreaks of gastrointestinal illness in all age-groups. The rapid onset and ease of person-to-person transmission suggest that inhibitors of the initial steps of virus binding to susceptible cells have value in limiting spread and outbreak persistence. We previously generated a monoclonal antibody (mAb 54.6 that blocks binding of recombinant norovirus-like particles (VLP to Caco-2 intestinal cells and inhibits VLP-mediated hemagglutination. In this study, we engineered the antigen binding domains of mAb 54.6 into a single chain variable fragment (scFv and tested whether these scFv could function as cell binding inhibitors, similar to the parent mAb. Results The scFv54.6 construct was engineered to encode the light (VL and heavy (VH variable domains of mAb 54.6 separated by a flexible peptide linker, and this recombinant protein was expressed in Pichia pastoris. Purified scFv54.6 recognized native VLPs by immunoblot, inhibited VLP-mediated hemagglutination, and blocked VLP binding to H carbohydrate antigen expressed on the surface of a CHO cell line stably transfected to express α 1,2-fucosyltransferase. Conclusion scFv54.6 retained the functional properties of the parent mAb with respect to inhibiting norovirus particle interactions with cells. With further engineering into a form deliverable to the gut mucosa, norovirus neutralizing antibodies represent a prophylactic strategy that would be valuable in outbreak settings.

  10. Production of in vivo biotinylated scFv specific to almond (Prunus dulcis) proteins by recombinant Pichia pastoris.

    Science.gov (United States)

    de la Cruz, Silvia; Alcocer, Marcos; Madrid, Raquel; García, Aina; Martín, Rosario; González, Isabel; García, Teresa

    2016-06-10

    The methylotropic yeast Pichia pastoris has demonstrated its suitability for large-scale production of recombinant proteins. As an eukaryotic organism P. pastoris presents a series of advantages at expression and processing of heterologous proteins when compared with Escherichia coli. In this work, P. pastoris has been used to express a scFv from a human synthetic library previously shown to bind almond proteins. In order to facilitate purification and post processing manipulations, the scFv was engineered with a C-terminal tag and biotinylated in vivo. After purification, biotinylated scFv were bound to avidin conjugated with HRP producing a multimeric scFv. The multimeric scFv showed to maintain their ability to recognize almond protein when assayed in ELISA, reaching a LOD of 470mgkg(-1). This study describes an easy method to produce large quantities of in vivo biotinylated scFv in P. pastoris. By substituting the enzyme or fluorochromes linked to avidin, it will be possible to generate a diverse number of multimeric scFv as probes to suit different analytical platforms in the detection of almond in food products. Copyright © 2016 Elsevier B.V. All rights reserved.

  11. [Construction and screening of phage antibody libraries against epidermal growth factor receptor and soluble expression of single chain Fv].

    Science.gov (United States)

    Sheng, Wei-Jin; Miao, Qing-Fang; Zhen, Yong-Su

    2009-06-01

    Recent studies have shown that epidermal growth factor receptor (EGFR) is an important target for cancer therapy. The present study prepared single chain Fv (scFv) directed against EGFR. Balb/c mice were immunized by human carcinoma A431 cells, and total RNA of the splenic cells was extracted. VH and VL gene fragments were amplified by RT-PCR and further joined into scFv gene with a linker, then scFv gene fragments were ligated into the phagemid vector pCANTAB 5E. The phagemid containing scFv were transformed into electro-competent E. coli TG1 cells. The recombinant phage antibody library was constructed through rescuing the transformed cells with help phage M13K07. The specified recombinant phages were enriched through 5 rounds of affinity panning and the anti-EGFR phage scFv clones were screened and identified with ELISA. A total of 48 clones from the library were selected randomly and 45 clones were identified positive. After infecting E. coli HB2151 cells with one positive clone, soluble recombinant antibodies about 27 kD were produced and located in the periplasm and the supernatant. The result of sequencing showed that the scFv gene was 768 bp, which encoded 256 amino acid residues. VH and VL including 3 CDRs and 4 FRs, respectively, were all homologous to mouse Ig. The soluble scFv showed the specific binding activity to purified EGFR and EGFR located in carcinoma cell membrane. The successful preparation of anti-EGFR scFv will provide an EGFR targeted molecule for the development of antibody-based drugs and biological therapy of cancer.

  12. Interstitial loop growth in electron irradiated vacuum-remelted FV448

    International Nuclear Information System (INIS)

    Buswell, J.T.; Fisher, S.B.

    1979-05-01

    An investigation of the resistance to void swelling of the ferritic steel FV448 has been made in electron irradiation experiments in a High Voltage Electron Microscope. Overaged samples of vacuum-remelted FV448 showed zero swelling after irradiation to doses of up to 30 dpa, in the temperature range 400 to 550 0 C. The resistance to void swelling was due to the absence of mutual interaction between interstitial loops. Impurity segregation to the loops, and precipitation, prevented continuous dislocation climb, thus removing biased sinks from the system at low doses. An interstitial-impurity binding energy of 0.5 eV was measured, suggesting that the impurity responsible was carbon or nitrogen. Some effects attributable to the heating of thin foils in the HVEM environment were detected. (author)

  13. Effects of developer exhaustion on DFL Contrast FV-58 and Kodak Insight dental films.

    Science.gov (United States)

    de Carvalho, Fabiano Pachêco; da Silveira, M M F; Frazão, M A G; de Santana, S T; dos Anjos Pontual, M L

    2011-09-01

    The aim of this study was to compare the properties of the DFL Contrast FV-58 F-speed film (DFL Co., Rio de Janerio, Brazil) with the Kodak Insight E/F speed film (Eastman Kodak, Rochester, NY) in fresh and exhausted processing solutions. The parameters studied were the speed, average gradient and latitude. Five samples of each type of film were exposed under standardized conditions over 5 weeks. The films were developed in fresh and progressively exhausted processing solutions. Characteristic curves were constructed from values of optical density and radiation dose and were used to calculate the parameters. An analysis of variance was performed separately for film type and time. DFL Contrast FV-58 film has a speed and average gradient that is significantly higher than Insight film, whereas the values of latitude are lower. Exhausted processing solutions were not significant in the parameters studied. DFL Contrast FV-58 film has stable properties when exhausted manual processing solutions are used and can be recommended for use in dental practice, contributing to dose reduction.

  14. [Preparation of monoclonal antibody against 4-amylphenol and homology modeling of its Fv fragment].

    Science.gov (United States)

    Cheng, Lei; Wu, Haizhen; Fei, Jing; Zhang, Lujia; Ye, Jiang; Zhang, Huizhan

    2017-03-01

    Objective To prepare and characterize a monoclonal antibody (mAb) against 4-amylphenol (4-AP), clone its cDNA sequence and make homology modeling for its Fv fragment. Methods A high-affinity anti-4-AP mAb was generated from a hybridoma cell line F10 using electrofusion between splenocytes from APA-BSA-immunized mouse and Sp2/0 myeloma cells. Then we extracted the mRNA of F10 cells and cloned the cDNA of mAb. The homology modeling and molecular docking of its Fv fragment was conducted with biological software. Results Under the optimum conditions, the ic-ELISA equation was y=A 2 +(A 1 -A 2 )/(1+(x/x 0 ) p ) (A 1 =1.28; A 2 =-0.066; x 0 =12560.75; p=0.74) with a correlation coefficient (R 2 ) of 0.997. The lowest detectable limit was 0.65 μg/mL. The heavy and light chains of mAb respectively belonged to IgG1 and Kappa. The homology modeling and molecular docking studies revealed that the binding of 4-Ap and mAb was attributed to the hydrogen bond and hydrophobic interactions. Conclusion The study successfully established a stable 4-AP mAb-secreting hybridoma cell line. The study on spatial structure of Fv fragment using homology modeling provided a reference for the development and design of single chain variable fragments.

  15. Characterization of a Single Chain Fv Antibody that Reacts with Free Morphine

    Directory of Open Access Journals (Sweden)

    Kazuhisa Sugimura

    2013-02-01

    Full Text Available An immune phage library derived from mice, hyperimmunized with morphine-conjugated BSA, was used to isolate a single-chain Fv (scFv clone, M86, with binding activity to morphine-conjugated thyroglobulin (morphine-C-Tg but not to codeine-, cocaine-, or ketamine-conjugated Tg. Surface plasmon resonance analysis using a morphine-C-Tg-coupled CM5 sensor chip showed that the Kd value was 1.26 × 10−8 M. To analyze its binding activity to free morphine and related compounds, we performed a competitive ELISA with M86 and morphine-C-Tg in the absence or presence of varying doses of free morphine and related compounds. IC50 values for opium, morphine, codeine, and heroin were 257 ng/mL, 36.4, 7.3, and 7.4 nM, respectively. Ketamine and cocaine exhibited no competitive binding activity to M86. Thus, we established a phage library-derived scFv, M86, which recognized not only free morphine and codeine as opium components but also heroin. This characteristic of M86 may be useful for developing therapeutic reagents for opiate addiction and as a free morphine-specific antibody probe.

  16. Performance of a small stand alone photovoltaic-wind system at El Oyameyo D.F., Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez Juarez, A.; Campos, J. [Centro de Investigacion en Energia, UNAM, Mexico, D. F. (Mexico); Tiburcio Silver, A. [Instituto Tecnologico de Toluca-Division de Posgrado, Toluca (Mexico)

    1997-12-31

    hibrido fotovoltaico-viento. La potencia electrica instalada para los sistemas FV autocontenidos estan en el rango de 48 W-p hasta 768 W-p. Entre estos hay 4 sistemas FV que estan configurados en sistemas regulados de CD y otros 6 son sistemas regulados de CA. Todos estos sistemas utilizan bancos de baterias de plomo-acido (selladas o ventiladas) para almacenar la energia producida diariamente por los sistemas. El sistema hibrido FV-Viento esta formado actualmente por un aerogenerador de 5.0 Kw, un arreglo FV de 768 W-p, un banco de baterias de almacenamiento de 37.8 Kw-h y un inversor de DC/AC de 5.0 Kw. En este articulo se reporta la electricidad generada, el patron de carga y el comportamiento general del sistema hibrido fotovoltaico-viento. Se presentan tambien las caracteristicas tecnicas, las pruebas de energia del sistema hibrido y la experiencia obtenida del manejo de la energia y del mantenimiento del sistema para todos los sistemas. Se encontro que todos los sistemas habian demostrado buen desempeno a satisfaccion del usuario.

  17. Performance of a small stand alone photovoltaic-wind system at El Oyameyo D.F., Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez Juarez, A; Campos, J [Centro de Investigacion en Energia, UNAM, Mexico, D. F. (Mexico); Tiburcio Silver, A [Instituto Tecnologico de Toluca-Division de Posgrado, Toluca (Mexico)

    1998-12-31

    hibrido fotovoltaico-viento. La potencia electrica instalada para los sistemas FV autocontenidos estan en el rango de 48 W-p hasta 768 W-p. Entre estos hay 4 sistemas FV que estan configurados en sistemas regulados de CD y otros 6 son sistemas regulados de CA. Todos estos sistemas utilizan bancos de baterias de plomo-acido (selladas o ventiladas) para almacenar la energia producida diariamente por los sistemas. El sistema hibrido FV-Viento esta formado actualmente por un aerogenerador de 5.0 Kw, un arreglo FV de 768 W-p, un banco de baterias de almacenamiento de 37.8 Kw-h y un inversor de DC/AC de 5.0 Kw. En este articulo se reporta la electricidad generada, el patron de carga y el comportamiento general del sistema hibrido fotovoltaico-viento. Se presentan tambien las caracteristicas tecnicas, las pruebas de energia del sistema hibrido y la experiencia obtenida del manejo de la energia y del mantenimiento del sistema para todos los sistemas. Se encontro que todos los sistemas habian demostrado buen desempeno a satisfaccion del usuario.

  18. Sistemas roboticos teleoperados

    Directory of Open Access Journals (Sweden)

    Alexander Ceron Correa

    2005-01-01

    Full Text Available En el presente artículo se realiza una revisión general sobre los sistemas robóticos teleoperados, su estructura, componentes principales, clasificación, tipos de sensores, sistemas de locomoción y aplicaciones; como ejemplo se mencionan algunos trabajos realizados Colombia y en el mundo

  19. Design and Generation of Humanized Single-chain Fv Derived from Mouse Hybridoma for Potential Targeting Application.

    Science.gov (United States)

    Khantasup, Kannika; Chantima, Warangkana; Sangma, Chak; Poomputsa, Kanokwan; Dharakul, Tararaj

    2015-12-01

    Single-chain variable antibody fragments (scFvs) are attractive candidates for targeted immunotherapy in several human diseases. In this study, a concise humanization strategy combined with an optimized production method for humanizing scFvs was successfully employed. Two antibody clones, one directed against the hemagglutinin of H5N1 influenza virus, the other against EpCAM, a cancer biomarker, were used to demonstrate the validity of the method. Heavy chain (VH) and light chain (VL) variable regions of immunoglobulin genes from mouse hybridoma cells were sequenced and subjected to the construction of mouse scFv 3-D structure. Based on in silico modeling, the humanized version of the scFv was designed via complementarity-determining region (CDR) grafting with the retention of mouse framework region (FR) residues identified by primary sequence analysis. Root-mean-square deviation (RMSD) value between mouse and humanized scFv structures was calculated to evaluate the preservation of CDR conformation. Mouse and humanized scFv genes were then constructed and expressed in Escherichia coli. Using this method, we successfully generated humanized scFvs that retained the targeting activity of their respective mouse scFv counterparts. In addition, the humanized scFvs were engineered with a C-terminal cysteine residue (hscFv-C) for site-directed conjugation for use in future targeting applications. The hscFv-C expression was extensively optimized to improve protein production yield. The protocol yielded a 20-fold increase in production of hscFv-Cs in E. coli periplasm. The strategy described in this study may be applicable in the humanization of other antibodies derived from mouse hybridoma.

  20. Sizing an isolated wind-solar-fuel cell generation system based on the particle swarm optimization method; Dimensionamiento de un sistema de generacion aislado eolico-solar-celda de combustible basado en el metodo de optimizacion de enjambre de particulas

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez-Huerta, V; Ramirez-Arredondo, Juan M. [Universidad de Quintana Roo, Chetumal, Quintana Roo (Mexico)]. E-mail: vsanchez@gdl.cinvestav.mx; Arriaga-Hurtado, L. G. [CIDETEQ, Queretaro (Mexico)

    2009-09-15

    Sizing an electric energy system requires an analysis of investment, maintenance and operating costs. In the case of a generation system that uses renewable sources, optimal capacity becomes more complex compared to a conventional system, because of the randomness of renewable resources (wind, solar) and the still high costs of wind and photovoltage generator modules. This work presents the optimal sizing of a wind-solar-fuel cell generation system, minimizing the costs of the system while satisfying the energy demands of an isolated charge. The optimization method used is based on an evolutionary programming technique known as particle swarms (PSO-particle swarm optimization). The generation of energy with a hybrid system is discussed, based on the profile of insolation and wind availability at the site, with the objective of satisfying a specific electric demand. [Spanish] El dimensionamiento de un sistema de generacion de energia electrica requiere un analisis de los costos de inversion, mantenimiento y operacion. En el caso de un sistema de generacion que utiliza fuentes renovables la capacidad optima resulta mas compleja con respecto a un sistema convencional, debido a la aleatoriedad de los recursos renovables (eolico, solar), y a los aun altos costos de generadores eolicos y modulos fotovoltaicos. En este trabajo se presenta el dimensionamiento optimo de un sistema de generacion eolico-solar-celda de combustible minimizando los costos del sistema que satisfaga la energia demandada por una carga aislada. El metodo de optimizacion utilizado esta basado en una tecnica de programacion evolutiva conocida como enjambre de particulas (PSO por sus siglas en ingles: particle swarm optimization). Se plantea la generacion de energia del sistema hibrido con base a la insolacion y el perfil del viento disponible en sitio, con objeto de satisfacer una demanda electrica determinada.

  1. Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells

    Science.gov (United States)

    Nie, Yong-Zhan; He, Feng-Tian; Li, Zhi-Kui; Wu, Kai-Chun; Cao, Yun-Xin; Chen, Bao-Jun; Fan, Dai-Ming

    2002-01-01

    AIM: To study the feasibility of panning and screening phage-displaying recombinant single-chain variable fragment (ScFv) of anti-tumor monoclonal antibodies for fixed whole cells as the carriers of mAb-binding antigens. METHODS: The recombinant phage displaying libraries for anti-colorectal tumor mAb MC3Ab, MC5Ab and anti-gastric tumor mAb MGD1 was constructed. Panning and screening were carried out by means of modified fixation of colorectal and gastric tumor cells expressed the mAb-binding antigens. Concordance of binding specificity to tumor cells between phage clones and parent antibodies was analyzed. The phage of positive clones was identified with competitive ELISA, and infected by E. coli HB2151 to express soluble ScFv. RESULTS: The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60%, 24% and 30%. MC3-ScFv had Mr 32000 confirmed by Western blot. The specificity to antigen had no difference between 4 positive recombinant phage antibodies and MC3Ab. CONCLUSION: The modified process of fixing whole tumor cells is efficient, convenient and feasible to pan and screen the phage-displaying ScFv of anti-tumor monoclonal antibodies. PMID:12174367

  2. en tres sistemas agroforestales

    Directory of Open Access Journals (Sweden)

    Javier Monge Meza

    2006-01-01

    Full Text Available Se estudió el desarrollo inicial (30 meses del duraznillo (Prunus annularis en tres sistemas agroforestales: duraznillo-maíz (Zea mays, duraznillomenta (Satureja viminea y duraznillo-naranjilla (Solanum quitoense, en un sitio en la zona de vida Bosque muy húmedo Montano Bajo, en Costa Rica. Se utilizaron cuatro parcelas de cada sistema agroforestal, con una densidad de 722 árboles/ ha, y un sistema de siembra de árboles en forma de triángulo equilátero, entre los que se intercaló el componente agrícola respectivo de cada sistema agroforestal. La altura media alcanzada por los árboles de duraznillo fue de 226,2, 221,4 y 164,3 cm, para el sistema agroforestal duraznillo-maíz, duraznillo- menta y duraznillo-naranjilla, respectivamente. En cuanto a los volúmenes, éstos variaron entre 0,58 y 1,30 m3/ha. Hubo una diferencia significativa en altura media entre el sistema agroforestal duraznillo-maíz y duraznillo-menta con respecto al de duraznillo-naranjilla. No hubo diferencias con respecto al volumen entre los sistemas de producción

  3. Numerical investigation on the prefabricated crack propagation of FV520B stainless steel

    Directory of Open Access Journals (Sweden)

    Juyi Pan

    Full Text Available FV520B is a common stainless steel for manufacturing centrifugal compressor impeller and shaft. The internal metal flaw destroys the continuity of the material matrix, resulting in the crack propagation fracture of the component, which seriously reduces the service life of the equipment. In this paper, Abaqus software was used to simulate the prefabricated crack propagation of FV520B specimen with unilateral gap. The results of static crack propagation simulation results show that the maximum value of stress–strain located at the tip of the crack and symmetrical distributed like a butterfly along the prefabricated crack direction, the maximum stress is 1990 MPa and the maximum strain is 9.489 × 10−3. The Mises stress and stress intensity factor KI increases with the increase of the expansion step, the critical value of crack initiation is reached at the 6th extension step. The dynamic crack propagation simulation shows that the crack propagation path is perpendicular to the load loading direction. Similarly, the maximum Mises stress located at the crack tip and is symmetrically distributed along the crack propagation direction. The critical stress range of the crack propagation is 23.3–43.4 MPa. The maximum value of stress–strain curve located at the 8th extension step, that is, the crack initiation point, the maximum stress is 55.22 MPa, and the maximum strain is 2.26 × 10−4. On the crack tip, the stress changed as 32.24–40.16 MPa, the strain is at 1.292 × 10−4–1.897 × 10−4. Keywords: FV520B, Crack propagation, Mises stress, Stress–strain, Numerical investigation

  4. Atlantic mackerel and Horse mackerel egg survey 2016: Dutch participation on board FV Atlantic Lady: May

    OpenAIRE

    Damme, van, C.J.G.

    2016-01-01

    From 10 till 25 May 2016 IMARES carried out a mackerel and horse mackerel egg survey on board the FV Atlantic Lady. This survey was part of the international mackerel and horse mackerel egg survey coordinated by ICES. The Redersvereniging voor de Zeevisserij (RVZ) asked IMARES to carry out this survey. Numbers of mackerel eggs in the samples were low, lower compared to previous surveys. Most mackerel eggs were found along the 200m depth contour of the continental slope. Most of the adult mack...

  5. Fab-dsFv: A bispecific antibody format with extended serum half-life through albumin binding.

    Science.gov (United States)

    Davé, Emma; Adams, Ralph; Zaccheo, Oliver; Carrington, Bruce; Compson, Joanne E; Dugdale, Sarah; Airey, Michael; Malcolm, Sarah; Hailu, Hanna; Wild, Gavin; Turner, Alison; Heads, James; Sarkar, Kaushik; Ventom, Andrew; Marshall, Diane; Jairaj, Mark; Kopotsha, Tim; Christodoulou, Louis; Zamacona, Miren; Lawson, Alastair D; Heywood, Sam; Humphreys, David P

    2016-10-01

    An antibody format, termed Fab-dsFv, has been designed for clinical indications that require monovalent target binding in the absence of direct Fc receptor (FcR) binding while retaining substantial serum presence. The variable fragment (Fv) domain of a humanized albumin-binding antibody was fused to the C-termini of Fab constant domains, such that the VL and VH domains were individually connected to the Cκ and CH1 domains by peptide linkers, respectively. The anti-albumin Fv was selected for properties thought to be desirable to ensure a durable serum half-life mediated via FcRn. The Fv domain was further stabilized by an inter-domain disulfide bond. The bispecific format was shown to be thermodynamically and biophysically stable, and retained good affinity and efficacy to both antigens simultaneously. In in vivo studies, the serum half-life of Fab-dsFv, 2.6 d in mice and 7.9 d in cynomolgus monkeys, was equivalent to Fab'-PEG.

  6. Construction of a Single Chain Variable Fragment Antibody (scFv) against Carbaryl and Its Interaction with Carbaryl.

    Science.gov (United States)

    Xiuyuan, Zhang; Zhihong, Huang; Lixia, Wang; Xiaonan, Liu

    2015-05-01

    Carbaryl is a low molecular weight insecticide that inhibits cholinesterase. Residues of carbaryl in food and the environment have damaged human health. A high-specificity scFv that can identify carbaryl is still lacking. In the present study, an anti-carbaryl scFv gene was prepared by cloning VL and VH genes from hybridoma cells secreting monoclonal antibody, then VH and VL were fused together using splicing by overlap extension (SOE) PCR with a flexible polypeptide linker connector (Gly4Ser)3, and then the scFv-pET-26b recombinant plasmid was constructed and transformed into E. coli BL21 for expression using IPTG as an inducer. The expressed recombinant protein was identified by SDS-PAGE and ELISA. The three-dimensional structure of the anti-carbaryl scFv was constructed by computer modeling, and carbaryl was docked to the scFv model to obtain the structure of the binding complex. The binding site was composed of Ala51, Ser52, Ile51, Gly54, Ser56, Arg98, and Gly100. This helps to understand the mechanism of interaction between anti-carbaryl antibody and antigen. Furthermore, it provides guidance for in vitro affinity maturation of anti-carbaryl antibody.

  7. Construction and sequencing analysis of scFv antibody fragment derived from monoclonal antibody against norfloxacin (Nor155

    Directory of Open Access Journals (Sweden)

    J. Mala

    2017-06-01

    Full Text Available Norfloxacin belongs to the group of fluoroquinolone antibiotics which has been approved for treatment in animals. However, its residues in animal products can pose adverse side effects to consumer. Therefore, detection of the residue in different food matrices must be concerned. In this study, a single chain variable fragment (scFv that recognizes norfloxacin antibiotic was constructed. The cDNA was synthesized from total RNA of hybridoma cells against norfloxacin. Genes encoding VH and VL regions of monoclonal antibody against norfloxacin (Nor155 were amplified and size of VH and VL fragments was 402 bp and 363 bp, respectively. The scFv of Nor155 was constructed by an addition of (Gly4Ser3 as a linker between VH and VL regions and subcloned into pPICZαA, an expression vector of Pichia pastoris. The sequence of scFv Nor155 (GenBank No. AJG06891.1 was confirmed by sequencing analysis. The complementarity determining regions (CDR I, II, and III of VH and VL were specified by Kabat method. The obtained recombinant plasmid will be useful for production of scFv antibody against norfloxacin in P. pastoris and further engineer scFv antibody against fluoroquinolone antibiotics.

  8. Single Chain Fv Constructs of Anti-Ganglioside GD2 Antibodies for Radioimaging and Radioimmunotherapy

    International Nuclear Information System (INIS)

    Cheung, Nai-Kong

    2003-01-01

    T-lymphocytes are ideal targeting vehicles because (1) they are naturally equipped with trafficking capabilities, (2) they can undergo clonal expansion when they come in contact with antigen if given the appropriate mitogenic signals, (3) they release cytokines which recruit other inflammatory/immune cells, (4) they can initiate other arms of immunity at the tumor site and (5) they are capable of being engineered with powerful cytotoxins or enzymes. Both clonal expansion and recruitment of T-cells can greatly magnify targeted delivery, improving the therapeutic index of the intended diagnostic or treatment modality. Although lymphokine activated killer cells (LAK) and tumor infiltrating lymphocytes (TIL) have been tested in tumor targeting, the clonal frequency of tumor-specific lymphocytes is generally very low and D2 -specific-scFv-CD28 chimeric immune receptor (CIR) into primary human peripheral blood CD8+ T-cells, which became selectively expanded when cultured with cells expressing both the MHC class I and G D2 . Thus, the transduced CIR carries out a functional costimulatory response that enhances their survival and selective expansion in the absence of natural costimulatory molecules. During the last funding period we have developed a novel affinity chromatography technique to rapidly clone efficient retroviral packaging cell lines. Using the pMSCVneo vector to carry the CIR and the packaging line GP+envAM 12, we can now transduce scFv-CD28-zeta-chain efficiently into primary human T-cells. The bulk culture achieves CIR monoclonality by 20 days of in vitro culture, achieving a 40-fold expansion in cell number within 2 months. The transduced T-cells kill tumors in vitro in an antigen specific manner and suppressed tumor growth when injected iv into SCID mice bearing human tumor xenografts. We have achieved CIR gene transduction in two separate antigen systems, one for GD2 (5F11 scFv) and one for the antigen p58 (8H9scFv). The novel antigen p58 was chosen

  9. Species-Dependent Functionality of the Human Cytolytic Fusion Proteins Granzyme B-H22(scFv and H22(scFv-Angiogenin in Macrophages

    Directory of Open Access Journals (Sweden)

    Theo Thepen

    2013-01-01

    Full Text Available Human cytolytic fusion proteins (hCFPs are comprised of a specific cell-surface-binding moiety and an effector molecule of human origin. In contrast to common immunotoxins, including bacterial or plant toxins, they are considered not to be immunogenic. Two examples for human pro-apoptotic effector proteins are the serine protease Granzyme B and the RNase Angiogenin. Pre-clinical testing of functionality in in vitro and in vivo studies is essential for therapeutics. Establishing relevant animal models that have predictive value for therapeutic success is a great challenge in biomedical research. In this study, we investigated the species-dependent cytotoxic activity of two hCFPs prior to their application in a murine inflammation model. We found that in vitro and ex vivo either hCFP was able to kill human cells only, leaving murine cells unaffected. In contrast, no species-dependency was found for the bacterial Pseudomonas exotoxin A based immunotoxin H22(scFv-ETA’. This species-dependent functioning has to be carefully considered when performing pre-clinical studies in animal models.

  10. Production of a germline-humanized cetuximab scFv and evaluation of its activity in recognizing EGFR- overexpressing cancer cells.

    Science.gov (United States)

    Banisadr, Arsham; Safdari, Yaghoub; Kianmehr, Anvarsadat; Pourafshar, Mahdieh

    2018-04-03

    The aim of this study was to produce a humanized single chain antibody (scFv) as a potential improved product design to target EGFR (Epidermal Growth Factor Receptor) overexpressing cancer cells. To this end, CDR loops of cetuximab (an FDA-approved anti-EGFR antibody) were grafted on framework regions derived from type 3 (VH3 and VL3 kappa) human germline sequences to obtain recombinant VH and VL domainslinked together with a flexible linker [(Gly 4 Ser) 3 ] to form a scFv. Codon optimized synthetic gene encoding the scFv (with NH2-VH-linker-VL-COOH orientation) was expressed in E. coli Origami™ 2(DE3) cells and the resultant scFv purified by using Ni-NTA affinity chromatography. The scFv, called cet.Hum scFv, was evaluated in ELISA and immunoblot to determine whether it can recognize EGFR. The scFv was able to recognize EGFR over-expressing cancer cells (A-431) but failed to detect cancer cells with low levels of EGFR (MCF-7 cells). Although the affinity of the scFv forA-431 cells was 9 fold lower than that of cetuximab, it was strong enough to recognize these cells. Considering its ability to bind EGFR molecules, the scFv may exhibit a potential application for the detection of EGFR-overexpressing cancer cells.

  11. Generation of functional scFv intrabody to abate the expression of CD147 surface molecule of 293A cells

    Directory of Open Access Journals (Sweden)

    Mai Sabine

    2008-01-01

    Full Text Available Abstract Background Expression of intracellular antibodies (intrabodies has become a broadly applicable technology for generation of phenotypic knockouts in vivo. The method uses surface depletion of cellular membrane proteins to examine their biological function. In this study, we used this strategy to block the transport of cell surface molecule CD147 to the cell membrane. Phage display technology was introduced to generate the functional antibody fragment to CD147, and we subsequently constructed a CD147-specific scFv that was expressed intracellularly and retained in the endoplasmic reticulum by adenoviral gene transfer. Results The recombinant antibody fragments, Fab and scFv, of the murine monoclonal antibody (clone M6-1B9 reacted specifically to CD147 by indirect enzyme-linked immunosorbent assays (ELISA using a recombinant CD147-BCCP as a target. This indicated that the Fab- and scFv-M6-1B9 displaying on phage surfaces were correctly folded and functionally active. We subsequently constructed a CD147-specific scFv, scFv-M6-1B9-intrabody, in 293A cells. The expression of CD147 on 293A cell surface was monitored at 36 h after transduction by flow cytometry and demonstrated remarkable reduction. Colocalization of scFv-M6-1B9 intrabody with CD147 in the ER network was depicted using a 3D deconvolution microscopy system. Conclusion The results suggest that our approach can generate antibody fragments suitable for decreasing the expression of CD147 on 293A cells. This study represents a step toward understanding the role of the cell surface protein, CD147.

  12. Identification and production of mouse scFv to specific epitope of enterovirus-71 virion protein-2 (VP2).

    Science.gov (United States)

    Thanongsaksrikul, Jeeraphong; Srimanote, Potjanee; Tongtawe, Pongsri; Glab-Ampai, Kittirat; Malik, Aijaz Ahmad; Supasorn, Oratai; Chiawwit, Phatcharaporn; Poovorawan, Yong; Chaicumpa, Wanpen

    2018-05-01

    Enterovirus-71 (EV71) and coxsackievirus-A16 (CA16) frequently cause hand-foot-mouth disease (HFMD) epidemics among infants and young children. CA16 infections are usually mild, while EV71 disease may be fatal due to neurologic complications. As such, the ability to rapidly and specifically recognize EV71 is needed to facilitate proper case management and epidemic control. Accordingly, the aim of this study was to generate antibodies to EV71-virion protein-2 (VP2) by phage display technology for further use in specific detection of EV71. A recombinant peptide sequence of EV71-VP2, carrying a predicted conserved B cell epitope fused to glutathione-S-transferase (GST) (designated GST-EV71-VP2/131-160), was produced. The fusion protein was used as bait in in-solution biopanning to separate protein-bound phages from a murine scFv (MuscFv) phage display library constructed from an immunoglobulin gene repertoire from naïve ICR mice. Three phage-transformed E. coli clones (clones 63, 82, and 83) produced MuscFvs that bound to the GST-EV71-VP2/131-160 peptide. The MuscFv of clone 83 (MuscFv83), which produced the highest ELISA signal to the target antigen, was further tested. MuscFv83 also bound to full-length EV71-VP2 and EV71 particles, but did not bind to GST, full-length EV71-VP1, or the antigenically related CA16. MuscFv83 could be a suitable reagent for rapid antigen-based immunoassay, such as immunochromatography (ICT), for the specific detection and/or diagnosis of EV71 infection as well as epidemic surveillance.

  13. Generation of human scFv antibody libraries: PCR amplification and assembly of light- and heavy-chain coding sequences.

    Science.gov (United States)

    Andris-Widhopf, Jennifer; Steinberger, Peter; Fuller, Roberta; Rader, Christoph; Barbas, Carlos F

    2011-09-01

    The development of therapeutic antibodies for use in the treatment of human diseases has long been a goal for many researchers in the antibody field. One way to obtain these antibodies is through phage-display libraries constructed from human lymphocytes. This protocol describes the construction of human scFv (single chain antibody fragment) libraries using a short linker (GGSSRSS) or a long linker (GGSSRSSSSGGGGSGGGG). In this method, the individual rearranged heavy- and light-chain variable regions are amplified separately and are linked through a series of overlap polymerase chain reaction (PCR) steps to give the final scFv products that are used for cloning.

  14. El Sistema inspirerede projekter

    DEFF Research Database (Denmark)

    Holst, Finn; Chemi, Tatiana

    Rapporten er resultat af pilotprojektet ”Forundersøgelse El Sistema DK” gennemført af Ph.D. Finn Holst (DPU, Aarhus Universitet) og Ph.D. Tatiana Chemi (Aalborg Universitet) i 2015. I rapporten indgår desuden bidrag fra Sofie Holm, Sara Fink Søndergaard og Stine Møgelbjerg Johansen. Pilotprojektet...... er gennemført for DMKLs El Sistema-udvalg med støtte fra Statens Kunstfond, Projektstøtteudvalget for Musik....

  15. Comparative response of platelet fV and plasma fV to activated protein C and relevance to a model of acute traumatic coagulopathy.

    Directory of Open Access Journals (Sweden)

    James E Campbell

    Full Text Available BACKGROUND: Acute traumatic coagulopathy (ATC has been linked to an increase in activated protein C (aPC from 40 pM in healthy individuals to 175 pM. aPC exerts its activity primarily through cleavage of active coagulation factor Va (fVa. Platelets reportedly possess fVa which is more resistant to aPC cleavage than plasma fVa; this work examines the hypothesis that normal platelets are sufficient to maintain coagulation in the presence of elevated aPC. METHODS: Coagulation responses of normal plasma, fV deficient plasma (fVdp, and isolated normal platelets in fVdp were conducted: prothrombin (PT tests, turbidimetry, and thromboelastography (TEG, including the dose response of aPC on the samples. RESULTS: PT and turbidimetric assays demonstrate that normal plasma is resistant to aPC at doses much higher than those found in ATC. Additionally, an average physiological number of washed normal platelets (200,000 platelets/mm3 was sufficient to eliminate the anti-coagulant effects of aPC up to 10 nM, nearly two orders of magnitude above the ATC concentration and even the steady-state pharmacological concentration of human recombinant aPC, as measured by TEG. aPC also demonstrated no significant effect on clot lysis in normal plasma samples with or without platelets. CONCLUSIONS: Although platelet fVa shows slightly superior resistance to aPC's effects compared to plasma fVa in static models, neither fVa is sufficiently cleaved in simulations of ATC or pharmacologically-delivered aPC to diminish coagulation parameters. aPC is likely a correlative indicator of ATC or may play a cooperative role with other activity altering products generated in ATC.

  16. Mechanical Characteristic of Remanufacturing of FV520B Precipitation Hardening Stainless Steel Using MAG Surfacing Deposition

    Directory of Open Access Journals (Sweden)

    LIU Jian

    2017-10-01

    Full Text Available Surfacing deposition forming method was adopted to carry out remanufacturing experiment of FV520B precipitation hardening stainless steel. Then the mechanical property characteristic of the remanufacturing layer was tested and studied, contrasted with the corresponding property of substrate. The results show that the remanufacturing layer, formed with MAG surfacing of FV520B precipitation hardening stainless steel has mechanical characteristic with high strength and hardness, the tensile strength reaches 1195MPa, exceeds 1092MPa of substrate, yield strength is 776MPa and average hardness is 336HV, is close to the corresponding property of substrate which is 859MPa and 353HV respectively; however, the elongation and impact toughness of the remanufacturing layer is merely 8.92% and 61J/cm2 respectively, it has a large gap with the corresponding property 19.72% and 144J/cm2 respectively of substrate. Fracture and microstructure analysis on specimens shows that the microstructure of remanufacturing layer is fast cooling non-equilibrium crystallized lath martensite, and carbide precipitated strengthening phase such as NbC, MoC, M23C6,etc, which is the reason that remanufacturing layer has high strength and high hardness. But as lack of aging treatment and Cu strengthening phase, and the weak interface between contaminating brittle phase or large size spherical particles and substrate will deteriorate the deformability and induce stress concentration and cracking when the material is load-carrying, and is the main reason of the remanufacturing layer having lower static tensile elongation and impact toughness.

  17. Temporal variation in community composition, pigmentation, and Fv/Fm of desert cyanobacterial soil crusts

    Science.gov (United States)

    Bowker, M.A.; Reed, S.C.; Belnap, J.; Phillips, S.L.

    2002-01-01

    Summers on the Colorado Plateau (USA) are typified by harsh conditions such as high temperatures, brief soil hydration periods, and high UV and visible radiation. We investigated whether community composition, physiological status, and pigmentation might vary in biological soil crusts as a result of such conditions. Representative surface cores were sampled at the ENE, WSW, and top microaspects of 20 individual soil crust pedicels at a single site in Canyonlands National Park, Utah, in spring and fall of 1999. Frequency of cyanobacterial taxa, pigment concentrations, and dark adapted quantum yield (Fv/Fm) were measured for each core. The frequency of major cyanobacterial taxa was lower in the fall compared to spring. The less-pigmented cyanobacterium Microcoleus vaginatus showed significant mortality when not in the presence of Nostoc spp. and Scytonema myochrous (Dillw.) Agardh. (both synthesizers of UV radiation-linked pigments) but had little or no mortality when these species were abundant. We hypothesize that the sunscreen pigments produced by Nostoc and Scytonema in the surface of crusts protect other, less-pigmented taxa. When fall and spring samples were compared, overall cyanobacterial frequency was lower in fall, while sunscreen pigment concentrations, chlorophyll a concentration, and Fv/Fm were higher in fall. The ratio of cyanobacterial frequency/chlorophyll a concentrations was 2-3 times lower in fall than spring. Because chlorophyll a is commonly used as a surrogate measure of soil cyanobacterial biomass, these results indicate that seasonality needs to be taken into consideration. In the fall sample, most pigments associated with UV radiation protection or repair were at their highest concentrations on pedicel tops and WSW microaspects, and at their lowest concentrations on ENE microaspects. We suggest that differential pigment concentrations between microaspects are induced by varying UV radiation dosage at the soil surface on these different

  18. El Sistema's Open Secrets

    Science.gov (United States)

    Booth, Eric

    2011-01-01

    In this article, the author talks about Venezuela's national youth orchestra program called El Sistema whose attributes offer a direct challenge to traditional Western music practices. As U.S. classical music--and all "high arts"--struggle to find relevance to more than the small "arts club" percentage of the U.S. populace, El…

  19. El sistema constructivo

    OpenAIRE

    Leser S., Heinz; Gómez Lerou, Luis; Salomone R., Vanessa

    2003-01-01

    Se describe el sistema constructivo de entramado en madera, “plataforma” (platform frame) utilizado preferentemente en las construcciones de varios pisos en el campamento minero de Sewell, Chile./The following is a description of the platform frame used in several stories buildings in the mining settlement Sewell, in Chile.

  20. El sistema constructivo

    Directory of Open Access Journals (Sweden)

    Leser S., Heinz

    2003-06-01

    Full Text Available Se describe el sistema constructivo de entramado en madera, “plataforma” (platform frame utilizado preferentemente en las construcciones de varios pisos en el campamento minero de Sewell, Chile./The following is a description of the platform frame used in several stories buildings in the mining settlement Sewell, in Chile.

  1. Engineering of a novel zipFv using leucine zipper motif against rabies virus glycoprotein G with improved protection potency in vivo.

    Science.gov (United States)

    Xi, Hualong; Zhang, Kaixin; Yin, Yanchun; Gu, Tiejun; Sun, Qing; Li, Zhuang; Cheng, Yue; Jiang, Chunlai; Kong, Wei; Wu, Yongge

    2017-06-01

    Rabies is an acute zoonotic infectious disease with a high fatality rate but is preventable with vaccination and rabies immunoglobulin (RIG). The single-chain Fv fragment (scFv), a small engineered antigen-binding protein derived from antibody variable heavy (V H ) and light (V L ) chains connected by a peptide linker, can potentially be used to replace RIG. Here, we produced two peptides V H -JUN-HIS and V L -FOS-HA separately in Escherichia coli and assembled them to form zipFv successfully in vitro. The new zipFv utilizes FOS and JUN leucine zippers to form an antibody structure similar to the IgG counterpart with two free N-terminal ends of V H and V L . The zipFv protein showed notable improvement in binding ability and affinity over its corresponding scFv. The zipFv also demonstrated greater stability in serum and the same protective rate as RIG against challenge with a standard rabies virus (CVS-24) in mice. Our results indicated zipFv as a novel and efficient antibody form with enhanced neutralizing potency. Copyright © 2017. Published by Elsevier B.V.

  2. A novel lentiviral scFv display library for rapid optimization and selection of high affinity antibodies.

    Science.gov (United States)

    Qudsia, Sehar; Merugu, Siva B; Mangukiya, Hitesh B; Hema, Negi; Wu, Zhenghua; Li, Dawei

    2018-04-30

    Antibody display libraries have become a popular technique to screen monoclonal antibodies for therapeutic purposes. An important aspect of display technology is to generate an optimization library by changing antibody affinity to antigen through mutagenesis and screening the high affinity antibody. In this study, we report a novel lentivirus display based optimization library antibody in which Agtuzumab scFv is displayed on cell membrane of HEK-293T cells. To generate an optimization library, hotspot mutagenesis was performed to achieve diverse antibody library. Based on sequence analysis of randomly selected clones, library size was estimated approximately to be 1.6 × 10 6 . Lentivirus display vector was used to display scFv antibody on cell surface and flow cytometery was performed to check the antibody affinity to antigen. Membrane bound scFv antibodies were then converted to secreted antibody through cre/loxP recombination. One of the mutant clones, M8 showed higher affinity to antigen in flow cytometery analysis. Further characterization of cellular and secreted scFv through western blot showed that antibody affinity was increased by three fold after mutagenesis. This study shows successful construction of a novel antibody library and suggests that hotspot mutagenesis could prove a useful and rapid optimization tool to generate similar libraries with various degree of antigen affinity. Copyright © 2018 Elsevier Inc. All rights reserved.

  3. FvSTR1, a striatin orthologue in Fusarium virguliforme, is required for asexual development and virulence.

    Science.gov (United States)

    Islam, Kazi T; Bond, Jason P; Fakhoury, Ahmad M

    2017-08-01

    The soil-borne fungus Fusarium virguliforme causes sudden death syndrome (SDS), one of the most devastating diseases of soybean in North and South America. Despite the importance of SDS, a clear understanding of the fungal pathogenicity factors that affect the development of this disease is still lacking. We have identified FvSTR1, a F. virguliforme gene, which encodes a protein similar to a family of striatin proteins previously reported to regulate signalling pathways, cell differentiation, conidiation, sexual development, and virulence in filamentous fungi. Striatins are multi-domain proteins that serve as scaffolding units in the striatin-interacting phosphatase and kinase (STRIPAK) complex in fungi and animals. To address the function of a striatin homologue in F. virguliforme, FvSTR1 was disrupted and functionally characterized using a gene knock out strategy. The resulting Fvstr1 mutants were largely impaired in conidiation and pigmentation, and displayed defective conidia and conidiophore morphology compared to the wild-type and ectopic transformants. Greenhouse virulence assays revealed that the disruption of FvSTR1 resulted in complete loss of virulence in F. virguliforme. Microtome studies using fluorescence microscopy showed that the Fvstr1 mutants were defective in their ability to colonize the vascular system. The Fvstr1 mutants also showed a reduced transcript level of genes involved in asexual reproduction and in the production of secondary metabolites. These results suggest that FvSTR1 has a critical role in asexual development and virulence in F. virguliforme.

  4. Construction of bifunctional molecules specific to antigen and antibody’s Fc-fragment by fusion of scFv-antibodies with staphylococcal protein A

    Directory of Open Access Journals (Sweden)

    Kolibo D. V.

    2009-06-01

    Full Text Available Aim. To develop approach for detection of scFv and their complexes with antigens. Methods. The fusion proteins, which include sequences of scFv and staphylococcal protein A, were constructed and the obtained bifunctional molecules were immunochemically analysed. Results. It was shown, that scFv fused with protein A and their complexes with antigens are effectively recognized by labelled immunoglobulins with unrestricted antigenic specificity. Conclusions. The fusion of scFv with protein A fragment is a perspective approach to increase the efficiency of application in ELISA. The obtained scFv, fused with protein A, could be used for development of test-systems for the detection of diphtheria toxin.

  5. Allergy immunotherapy with a hypoallergenic recombinant birch pollen allergen rBet v 1-FV in a randomized controlled trial.

    Science.gov (United States)

    Klimek, Ludger; Bachert, Claus; Lukat, Karl-Friedrich; Pfaar, Oliver; Meyer, Hanns; Narkus, Annemie

    2015-01-01

    Pollen extracts and chemically modified allergoids are used successfully in allergen immunotherapy (AIT). Recombinant extracts offer potential advantages with respect to pharmaceutical quality, standardization and dosing. A hypoallergenic recombinant folding variant of the major birch pollen allergen (rBet v 1-FV) was compared with an established native birch preparation. A pre-seasonal, randomized, actively controlled phase II study was performed in birch pollen allergic rhino-conjunctivitis with or without asthma, GINA I/ II. 51 patients (24 rBet v 1-FV, 27 native extract) started therapy with subcutaneous allergen immunotherapy (SCIT). Primary end-point was a combined symptom medication score (SMS), changes in nasal provocation test, visual rating score and specific antibody responses secondary end-points. After one pre-seasonal treatment course the combined SMS was 5.86 (median; IQR: 14.02) for the rBet v 1-FV group versus 12.40 (median; IQR: 9.32) for the comparator during the three weeks pollen season (p = 0.330). After treatment in the second year, scores were 3.00 (median; IQR: 6.50) and 2.93 (4.86) respectively. Allergen tolerance in a nasal provocation test improved to a comparable extent in both groups. Significant increases in birch pollen-specific IgG1 and IgG4 were observed in both treatment groups following the first treatment phase and remained significantly raised until the end of the study. In this first in man, proof of concept phase II trial no statistical difference between rBet v 1-FV and an established natural pollen extract could be observed. rBet v 1-FV could be administered in higher doses than the native protein with no increase in adverse effects. The study was registered in clinicalTrials.gov (NCT00266526).

  6. Therapeutic monoclonal antibodies: scFv patents as a marker of a new class of potential biopharmaceuticals

    Directory of Open Access Journals (Sweden)

    Manuela Berto Pucca

    2011-03-01

    Full Text Available Monoclonal antibodies represent the fastest growing class of biopharmaceutical products and have a host of applications in medical research, diagnosis, therapy, and basic science. The production of recombinant monoclonal antibodies has revolutionized the generation of immunoglobulins, and their use represents a strategic breakthrough, affecting the global pharmaceutical market for therapeutic proteins. In the present work, a review of scFv, and the number of related patents, has been carried out. The results show that several countries have scFv patents, most notably the United States, China and United Kingdom. The target of these scFv antibodies was also assessed and the results demonstrate that most are directed toward cancer therapy.Anticorpos monoclonais representam a classe de maior crescimento em produtos de biofármacos e possuem várias aplicações em pesquisa médica, diagnóstico, terapias e ciência básica. A produção de anticorpos monoclonais recombinantes revolucionou a geração de imunoglobulinas e sua utilização implica em avanço estratégico, afetando o mercado farmacêutico global de proteínas terapêuticas. No presente trabalho, uma revisão sobre scFv e a relação do seu número de patentes foi analisada. Os resultados mostram que vários países apresentam patentes de scFv com destaque para os Estados Unidos, China e Reino Unido. Os alvos desses anticorpos também foram avaliados e as análises revelaram que a maioria é destinado a terapias contra o câncer.

  7. experiencias en el sistema

    Directory of Open Access Journals (Sweden)

    B. Fernández Manjón

    2005-01-01

    Full Text Available En este trabajo se presenta la experiencia de aplicación e implementación del modelo de objetos educativos en el sistema . Se describe una visión práctica de la aplicación de las tecnologías de marcado tanto en la estructuración de los cursos como en el desarrollo de contenidos que pueden ser adaptados al usuario. En se han seguido las especificaciones de IMS, desarrollándose un soporte completo del empaquetamiento de cursos (content packaging reflejado en un sistema de autoría de cursos y una herramienta de importación. Además se ha particularizado un perfil específico de cursos y de objetos educativos que permiten su adaptación al usuario.

  8. Sistemas integrados con Arduino

    OpenAIRE

    EL YAKOUTI, MOHAMMED

    2017-01-01

    Design of a robot prototype remotely controllable from Bluetooth using Arduino. Control and testing of sensors and events interacting with Arduino and Bluetooth. Diseño de un prototipo de robot controlable remotamente con Bluetooth utilizando Arduino. Control y verificación de los sensores y eventos que interactúan mediante el Arduino y el Bluetooth. El Yakouti, M. (2017). Sistemas integrados con Arduino. http://hdl.handle.net/10251/89274. TFGM

  9. SISTEMA DE MONITORAMENTO AUTOMATIZADO

    Directory of Open Access Journals (Sweden)

    Raiane viana Souza

    2016-11-01

    Full Text Available Atualmente existe uma grande necessidade de ter sistemas que auxiliem no monitoramento de ambientes, por exemplo: uma residência; permitindo assim que o usuário possa controlar e ter acesso a tudo que acontece quando o mesmo estiver ausente. Este projeto propõe realizar o desenvolvimento de um sistema de monitoramento automatizado, cujo objetivo é identificar a presença de pessoas e capturar temperaturas de um ambiente. O sistema é composto por um microcontrolador Arduíno, no qual estará acoplado: uma Webcam, sensores de presença e temperatura e um módulo wifi, que possibilitará o envio dos dados capturados ao servidor web. No protótipo desenvolvido foram implantados todos os recursos definidos com base nos requisitos do usuário, dentre os quais podem-se destacar: permitir detectar a presença de pessoas em ambiente, capturar imagens, capturar temperatura em um intervalo de tempo específico e enviar todos os dados para o servidor; permitir que o usuário visualize as informações capturadas através de um site; gerar relatórios e gráficos com as informações capturadas e permitir que o usuário exporte as informações em formato de arquivo.

  10. Sistemas Silvipastoris Silvipastoral Systems

    Directory of Open Access Journals (Sweden)

    Fernando Salgado Bernardino

    2010-02-01

    Full Text Available

    Apesar das pesquisas com sistemas silvipastoris terem sido iniciadas no final da década de 1970, as informações geradas até hoje não são em grande número. As vantagens advindas de um sistema silvipastoril são inúmeras e devidamente reconhecidas. Pelo fato de ser uma técnica de uso da terra capaz de recuperar ecossistemas alterados pelo mau manejo, estes sistemas, pela integração de atividades agrícolas, pecuárias e silviculturais, passam a representar uma tecnologia que confere maior sustentabilidade que os sistemas tradicionais, nos quais os monocultivos são predominantes. Nos últimos anos, instituições de ensino, pesquisa e extensão do País têm se voltado para o desenvolvimento de tais sistemas. O governo de Minas Gerais, por meio de sua Secretaria de Estado de Agricultura e de órgãos vinculados, vem promovendo a condução de modelos agrossilvipastoris num processo integrado de ocupação do solo, denominado lavoura-pecuária-silvicultura. Apesar dos grandes avanços no conhecimento de culturas anuais e dos componentes arbóreos, principalmente o eucalipto, e de gramíneas forrageiras tolerantes à diminuição da intensidade luminosa, ainda é necessário se comnhecer melhor o manejo do sub-bosque sob os efeitos do pastejo.

     

    doi: 10.4336/2009.pfb.60.77

    Although researches related to silvopastoral systems have been initiated many years ago in Brazil, there are few informations generated until now. The advantages from a silvopastoral system are inumerous and well recognized. By the fact of being a technique of land using capable of recovering several disturbed ecosystems, the silvopastoral systems by the integration of agricultural, cattle, and silvicultural activities represent a technology that can achieve higher sustainability than the traditional systems as the monocultures. Today, research centers in Brazil dedicate great attention to agrossilvopastoral systems. The government of Minas Gerais

  11. FV-100 versus valacyclovir for the prevention of post-herpetic neuralgia and the treatment of acute herpes zoster-associated pain: A randomized-controlled trial.

    Science.gov (United States)

    Tyring, Stephen K; Lee, Patricia; Hill, Gordon T; Silverfield, Joel C; Moore, Angela Yen; Matkovits, Theresa; Sullivan-Bolyai, John

    2017-07-01

    This prospective, parallel-group, randomized, double-blind, multicenter study compared the efficacy and safety of FV-100 with valacyclovir for reducing pain associated with acute herpes zoster (HZ). Patients, ≥50 years of age, diagnosed with HZ within 72 h of lesion appearance who had HZ-associated pain, were randomized 1:1:1 to a 7-day course of either FV-100 200 mg QD (n = 117), FV-100 400 mg QD (n = 116), or valacyclovir 1000 mg TID (n =117). Efficacy was evaluated on the basis of the burden of illness (BOI; Zoster Brief Pain Inventory scores); incidence and duration of clinically significant pain (CSP); pain scores; incidence and severity of post-herpetic neuralgia (PHN); and times to full lesion crusting and to lesion healing. Safety was evaluated on the basis of adverse event (AE)/SAE profiles, changes in laboratory and vital signs values, and results of electrocardiograms. The burden of illness scores for pain through 30 days were 114.5, 110.3, and 118.0 for FV-100 200 mg, FV-100 400 mg, and valacyclovir 3000 mg, respectively. The incidences of PHN at 90 days for FV-100 200 mg, FV-100 400 mg, and valacyclovir 3000 mg were 17.8%, 12.4%, and 20.2%, respectively. Adverse event and SAE profiles of the two FV-100 and the valacyclovir groups were similar and no untoward signals or trends were evident. These results demonstrate a potential for FV-100 as an antiviral for the treatment of shingles that could both reduce the pain burden of the acute episode and reduce the incidence of PHN compared with available treatments. © 2016 Wiley Periodicals, Inc.

  12. Screening of synthetic phage display scFv libraries yields competitive ligands of human leptin receptor.

    Science.gov (United States)

    Molek, Peter; Vodnik, Miha; Strukelj, Borut; Bratkovič, Tomaž

    2014-09-26

    Initially considered the main endogenous anorexigenic factor, fat-derived leptin turned out to be a markedly pleiotropic hormone, influencing diverse physiological processes. Moreover, hyperleptinemia in obese individuals has been linked to the onset or progression of serious disorders, such as cancer, autoimmune diseases, and atherosclerosis, and antagonizing peripheral leptin's signalization has been shown to improve these conditions. To develop an antibody-based leptin antagonist we have devised a tailored panning procedure and screened two phage display libraries of single chain variable antibody fragments (scFvs) against recombinant leptin receptor. One of the scFvs was expressed in Escherichia coli and its interaction with leptin receptor was characterized in more detail. It was found to recognize a discontinuous epitope and to compete with leptin for receptor binding with IC50 and Kd values in the nanomolar range. The reported scFv represents a lead for development of leptin antagonists that may ultimately find use in therapy of various hyperleptinemia-related disorders. Copyright © 2014 Elsevier Inc. All rights reserved.

  13. Clinical Applications of Phage-Derived sFvs and sFv Fusion Proteins

    Directory of Open Access Journals (Sweden)

    K. A. Chester

    2000-01-01

    Full Text Available Single chain Fv antibodies (sFvs have been produced from filamentous bacteriophage libraries obtained from immunised mice. MFE-23, the most characterised of these sFvs, is reactive with carcinoembryonic antigen (CEA, a glycoprotein that is highly expressed in colorectal adenocarcinomas. MFE-23 has been expressed in bacteria and purified in our laboratory for two clinical trials; a gamma camera imaging trial using 123I-MFE-23 and a radioimmunoguided surgery trial using 125I-MFE-23, where tumour deposits are detected by a hand-held probe during surgery. Both these trials show MFE-23 is safe and effective in localising tumour deposits in patients with cancer. We are now developing fusion proteins which use MFE-23 to deliver a therapeutic moiety; MFE-23::CPG2 targets the enzyme carboxypeptidase G2 (CPG2 for use in the ADEPT (antibody directed enzyme prodrug therapy system and MFE::TNFα aims to reduce sequestration and increase tumor concentrations of systemically administered TNFα.

  14. Radiosensitization and growth inhibition of cancer cells mediated by an scFv antibody gene against DNA-PKcs in vitro and in vivo

    International Nuclear Information System (INIS)

    Du, Li; Zhou, Ping-Kun; Zhou, Li-Jun; Pan, Xiu-Jie; Wang, Yu-Xiao; Xu, Qin-Zhi; Yang, Zhi-Hua; Wang, Yu; Liu, Xiao-Dan; Zhu, Mao-Xiang

    2010-01-01

    Overexpression of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is commonly occurred in cancers and causes radioresistance and poor prognosis. In present study, the single-chain variable antibody fragments (scFv) targeting DNA-PKcs was developed for the application of radiosensitization in vitro and in vivo. A humanized semisynthetic scFv library and the phage-display antibodies technology were employed to screen DNA-PKcs scFv antibody. DNA-PKcs epitopes were predicted and cloned. A humanized semisynthetic scFv library and the phage-display antibodies technology were employed to screen DNA-PKcs scFv antibody. DNA damage repair was analyzed by comet assay and immunofluorescence detection of γH2AX foci. The radiosensitization in vivo was determined on Balb/c athymic mice transplanted tumours of HeLa cells. Four epitopes of DNA-PKcs have been predicted and expressed as the antigens, and a specific human anti-DNA-PKcs scFv antibody gene, anti-DPK3-scFv, was obtained by screening the phage antibody library using the DNA-PKcs peptide DPK3. The specificity of anti-DPK3-scFv was verified, in vitro. Transfection of HeLa cells with the anti-DPK3-scFv gene resulted in an increased sensitivity to IR, decreased repair capability of DNA double-strand breaks (DSB) detected by comet assay and immunofluorescence detection of γH2AX foci. Moreover, the kinase activity of DNA-PKcs was inhibited by anti-DPK3-scFv, which was displayed by the decreased phosphorylation levels of its target Akt/S473 and the autophosphorylation of DNA-PKcs on S2056 induced by radiation. Measurement of the growth and apoptosis rates showed that anti-DPK3-scFv enhanced the sensitivity of tumours transplanted in Balb/c athymic mice to radiation therapy. The antiproliferation and radiosensitizing effects of anti-DPK3-scFv via targeting DNA-PKcs make it very appealing for the development as a novel biological radiosensitizer for cancer therapeutic potential

  15. Development of single chain variable fragment (scFv) antibodies against Xylella fastidiosa subsp. pauca by phage display.

    Science.gov (United States)

    Yuan, Qing; Jordan, Ramon; Brlansky, Ronald H; Istomina, Olga; Hartung, John

    2015-10-01

    Xylella fastidiosa is a member of the gamma proteobacteria. It is fastidious, insect-vectored and xylem-limited and causes a variety of diseases, some severe, on a wide range of economically important perennial crops, including grape and citrus. Antibody based detection assays are commercially available for X. fastidiosa, and are effective at the species, but not at the subspecies level. We have made a library of scFv antibody fragments directed against X. fastidiosa subsp. pauca strain 9a5c (citrus) by using phage display technology. Antibody gene repertoires were PCR-amplified using 23 primers for the heavy chain variable region (V(H)) and 21 primers for the light chain variable region (V(L)). The V(H) and V(L) were joined by overlap extension PCR, and then the genes of the scFv library were ligated into the phage vector pKM19. The library contained 1.2×10(7) independent clones with full-length scFv inserts. In each of 3cycles of affinity-selection with 9a5c, about 1.0×10(12) phage were used for panning with 4.1×10(6), 7.1×10(6), 2.1×10(7) phage recovered after the first, second and third cycles, respectively. Sixty-six percent of clones from the final library bound X. fastidiosa 9a5c in an ELISA. Some of these scFv antibodies recognized strain 9a5c and did not recognize X. fastidiosa strains that cause Pierce's disease of grapevine. Published by Elsevier B.V.

  16. Experimental study on stress corrosion crack propagation rate of FV520B in carbon dioxide and hydrogen sulfide solution

    Directory of Open Access Journals (Sweden)

    Ming Qin

    Full Text Available FV520B steel is a kind of precipitation hardening Martensitic stainless steel, it has high-strength, good plasticity and good corrosion resistance. Stress corrosion cracking (SCC is one of the main corrosion failure mode for FV520B in industrial transportation of natural gas operation. For a better understanding the effect on SCC of FV520B, the improved wedge opening loading (WOL specimens and constant displacement loading methods were employed in experimental research in carbon dioxide and hydrogen sulfide solution. The test results showed that the crack propagation rate is 1.941 × 10−7–5.748 × 10−7 mm/s, the stress intensity factor KISCC is not more than 36.83 MPa m. The rate increases with the increasing of the crack opening displacement. Under the condition of different initial loading, KISCC generally shows a decreasing tendency with the increase in H2S concentration, and the crack propagation rate showed an increasing trend substantially. For the enrichment of sulfur ion in the crack tip induced the generation of pitting corrosion, promoting the surrounding metal formed the corrosion micro batteries, the pit defects gradually extended and connected with the adjacent pit to form a small crack, leading to further propagation till cracking happened. Fracture microscopic morphology displayed typical brittle fracture phenomena, accompanying with trans-granular cracking, river shape and sector, many second cracks on the fracture surface. Keywords: FV520B, Wedge opening loading specimen, Stress corrosion cracking, Hydrogen sulfide

  17. Experimental study on stress corrosion crack propagation rate of FV520B in carbon dioxide and hydrogen sulfide solution

    Science.gov (United States)

    Qin, Ming; Li, Jianfeng; Chen, Songying; Qu, Yanpeng

    FV520B steel is a kind of precipitation hardening Martensitic stainless steel, it has high-strength, good plasticity and good corrosion resistance. Stress corrosion cracking (SCC) is one of the main corrosion failure mode for FV520B in industrial transportation of natural gas operation. For a better understanding the effect on SCC of FV520B, the improved wedge opening loading (WOL) specimens and constant displacement loading methods were employed in experimental research in carbon dioxide and hydrogen sulfide solution. The test results showed that the crack propagation rate is 1.941 × 10-7-5.748 × 10-7 mm/s, the stress intensity factor KISCC is not more than 36.83 MPa √{ m } . The rate increases with the increasing of the crack opening displacement. Under the condition of different initial loading, KISCC generally shows a decreasing tendency with the increase in H2S concentration, and the crack propagation rate showed an increasing trend substantially. For the enrichment of sulfur ion in the crack tip induced the generation of pitting corrosion, promoting the surrounding metal formed the corrosion micro batteries, the pit defects gradually extended and connected with the adjacent pit to form a small crack, leading to further propagation till cracking happened. Fracture microscopic morphology displayed typical brittle fracture phenomena, accompanying with trans-granular cracking, river shape and sector, many second cracks on the fracture surface.

  18. Sistema de Reconocimiento de Caracteres Orientado a Sistemas Android

    OpenAIRE

    Segura Morales, Oriol; Universitat Autònoma de Barcelona. Escola d'Enginyeria

    2014-01-01

    Este proyecto trata sobre un sistema de reconocimiento de caracteres que se implementa para sistemas Android en forma de aplicación para móvil y Tablet. La aplicación está orientada para que los niños aprendan a escribir de una forma más amena. El sistema utiliza el patrón de diseño Modelo-Vista-Controlador para separar la lógica del sistema de la interfaz visual. En la interfaz, el sistema pide que se escriba un carácter utilizando la pantalla táctil y una vez el usuario lo ha escrito el sis...

  19. Sistema seguidor solar microcontrolado

    Directory of Open Access Journals (Sweden)

    Daiane Maria Mendes Duarte

    2015-12-01

    Full Text Available A demanda de energia elétrica é cada vez maior devido ao grande crescimento da população e do novo estilo de vida adotado pela sociedade moderna, cada vez mais industrializada. Nesse sentido, o consumo de energia vem apresentando um crescimento acelerado. Concomitantemente, a sociedade se movimenta no sentido de se conscientizar das alterações ocasionadas na natureza, e, assim, surge a necessidade de novos meios de geração de energia, menos impactantes ao meio ambiente, denominadas energias renováveis. Este artigo vem apresentar um sistema seguidor solar microcontrolado, que possibilita uma maior captação de energia em placas solares, pois as posiciona sempre com sua face voltada para o sol. O sistema de controle é baseado nas equações matemáticas cujos resultados são as posições do sol num dado dia e em uma dada hora. Estas coordenadas são calculadas e resultam no deslocamento da célula fotovoltaica por meio de um motor.

  20. Coupled fvGCM-GCE Modeling System, 3D Cloud-Resolving Model and Cloud Library

    Science.gov (United States)

    Tao, Wei-Kuo

    2005-01-01

    Recent GEWEX Cloud System Study (GCSS) model comparison projects have indicated that cloud- resolving models (CRMs) agree with observations better than traditional single-column models in simulating various types of clouds and cloud systems from different geographic locations. Current and future NASA satellite programs can provide cloud, precipitation, aerosol and other data at very fine spatial and temporal scales. It requires a coupled global circulation model (GCM) and cloud-scale model (termed a super-parameterization or multi-scale modeling framework, MMF) to use these satellite data to improve the understanding of the physical processes that are responsible for the variation in global and regional climate and hydrological systems. The use of a GCM will enable global coverage, and the use of a CRM will allow for better and more sophisticated physical parameterization. NASA satellite and field campaign cloud related datasets can provide initial conditions as well as validation for both the MMF and CRMs. A seed fund is available at NASA Goddard to build a MMF based on the 2D Goddard Cumulus Ensemble (GCE) model and the Goddard finite volume general circulation model (fvGCM). A prototype MMF in being developed and production runs will be conducted at the beginning of 2005. In this talk, I will present: (1) A brief review on GCE model and its applications on precipitation processes, ( 2 ) The Goddard MMF and the major difference between two existing MMFs (CSU MMF and Goddard MMF), (3) A cloud library generated by Goddard MMF, and 3D GCE model, and (4) A brief discussion on the GCE model on developing a global cloud simulator.

  1. Human scFv antibodies (Afribumabs) against Africanized bee venom: Advances in melittin recognition.

    Science.gov (United States)

    Pessenda, Gabriela; Silva, Luciano C; Campos, Lucas B; Pacello, Elenice M; Pucca, Manuela B; Martinez, Edson Z; Barbosa, José E

    2016-03-15

    Africanized Apis mellifera bees, also known as killer bees, have an exceptional defensive instinct, characterized by mass attacks that may cause envenomation or death. From the years 2000-2013, 77,066 bee accidents occurred in Brazil. Bee venom comprises several substances, including melittin and phospholipase A2 (PLA2). Due to the lack of antivenom for bee envenomation, this study aimed to produce human monoclonal antibody fragments (single chain fragment variable; scFv), by using phage display technology. These fragments targeted melittin and PLA2, the two major components of bee venom, to minimize their toxic effects in cases of mass envenomation. Two phage antibody selections were performed using purified melittin. As the commercial melittin is contaminated with PLA2, phages specific to PLA2 were also obtained during one of the selections. Specific clones for melittin and PLA2 were selected for the production of soluble scFvs, named here Afribumabs: prefix: afrib- (from Africanized bee); stem/suffix: -umab (fully human antibody). Afribumabs 1 and 2 were tested in in vitro and in vivo assays to assess their ability to inhibit the toxic actions of purified melittin, PLA2, and crude bee venom. Afribumabs reduced hemolysis caused by purified melittin and PLA2 and by crude venom in vitro and reduced edema formation in the paws of mice and prolonged the survival of venom-injected animals in vivo. These results demonstrate that Afribumabs may contribute to the production of the first non-heterologous antivenom treatment against bee envenomation. Such a treatment may overcome some of the difficulties associated with conventional immunotherapy techniques. Copyright © 2016 Elsevier Ltd. All rights reserved.

  2. Histone H3 lysine 9 methyltransferase FvDim5 regulates fungal development, pathogenicity and osmotic stress responses in Fusarium verticillioides.

    Science.gov (United States)

    Gu, Qin; Ji, Tiantian; Sun, Xiao; Huang, Hai; Zhang, Hao; Lu, Xi; Wu, Liming; Huo, Rong; Wu, Huijun; Gao, Xuewen

    2017-10-16

    Histone methylation plays important biological roles in eukaryotic cells. Methylation of lysine 9 at histone H3 (H3K9me) is critical for regulating chromatin structure and gene transcription. Dim5 is a lysine histone methyltransferase (KHMTase) enzyme, which is responsible for the methylation of H3K9 in eukaryotes. In the current study, we identified a single ortholog of Neurospora crassa Dim5 in Fusarium verticillioides. In this study, we report that FvDim5 regulates the trimethylation of H3K9 (H3K9me3). The FvDIM5 deletion mutant (ΔFvDim5) showed significant defects in conidiation, perithecium production and fungal virulence. Unexpectedly, we found that deletion of FvDIM5 resulted in increased tolerance to osmotic stresses and upregulated FvHog1 phosphorylation. These results indicate the importance of FvDim5 for the regulation of fungal development, pathogenicity and osmotic stress responses in F. verticillioides. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  3. Multinuclear NMR study of the structure of the Fv fragment of anti-dansyl mouse IgG2a antibody

    Energy Technology Data Exchange (ETDEWEB)

    Takahashi, Hideo; Odaka, Asano; Matsunaga, Chigusa; Kato, Koichi; Shimada, Ichio; Arata, Yoji (Univ. of Tokyo (Japan)); Kawaminami, Shunro (Kao Corp., Tochigi (Japan))

    1991-07-02

    A multinuclear NMR study is reported of Fv, which is a minimum antigen-binding unit of immunoglobulin. Fv has been prepared by clostripain digestion of a mouse anti-dansyl IgG2a monoclonal antibody that lacks the entire C{sub H}1 domain. A variety of Fv analogues labeled with {sup 2}H in the aromatic rings and with {sup 13}C and/or {sup 15}N in the peptide bonds have been prepared and used for multinuclear NMR analyses of Fv spectra of Fv sensitively reflect the antigen binding and can be used along with {sup 1}H and {sup 13}C spectral data for the structural analyses of antigen-antibody interactions. Hydrogen-deuterium exchange of the amide protons has been folowed in the absence and presence of DNS-Lys by using the {sup 1}H-{sup 15}N shift correlation spectra. Use of the {beta}-shift observed for the carbonyl carbon resonances has also been helpful in following the hydrogen-deuterium exchange. On the basis of the NMR data obtained, the static and dynamic structure of the Fv fragment in the absence and presence of DNS-Lys has been discussed.

  4. Multinuclear NMR study of the structure of the Fv fragment of anti-dansyl mouse IgG2a antibody

    International Nuclear Information System (INIS)

    Takahashi, Hideo; Odaka, Asano; Matsunaga, Chigusa; Kato, Koichi; Shimada, Ichio; Arata, Yoji; Kawaminami, Shunro

    1991-01-01

    A multinuclear NMR study is reported of Fv, which is a minimum antigen-binding unit of immunoglobulin. Fv has been prepared by clostripain digestion of a mouse anti-dansyl IgG2a monoclonal antibody that lacks the entire C H 1 domain. A variety of Fv analogues labeled with 2 H in the aromatic rings and with 13 C and/or 15 N in the peptide bonds have been prepared and used for multinuclear NMR analyses of Fv spectra of Fv sensitively reflect the antigen binding and can be used along with 1 H and 13 C spectral data for the structural analyses of antigen-antibody interactions. Hydrogen-deuterium exchange of the amide protons has been folowed in the absence and presence of DNS-Lys by using the 1 H- 15 N shift correlation spectra. Use of the β-shift observed for the carbonyl carbon resonances has also been helpful in following the hydrogen-deuterium exchange. On the basis of the NMR data obtained, the static and dynamic structure of the Fv fragment in the absence and presence of DNS-Lys has been discussed

  5. Study of a fuel cell insertion in a hybrid system for energy generation; Estudo da insercao de celula a combustivel integrada a sistema hibrido de geracao de eletricidade isolado

    Energy Technology Data Exchange (ETDEWEB)

    Vale, Silvio Bispo do; Bezerra, Ubiratan Holanda; Pinho, Joao Tavares; Pereira, Edinaldo Jose da Silva [Universidade Federal do Para (UFPA), Belem, PA (Brazil). Programa de Pos-Graduacao em Engenharia Eletrica. Grupo de Estudo e Desenvolvimento em Alternativas Energeticas

    2004-07-01

    This paper presents a case study of a fuel cell (FC) insertion in a small hybrid system for energy generation composed by wind (10 kW), photovoltaic (3,2 kW) and diesel (20 kVA) generations, making computer simulations in order to evaluate the contribution of each one of the energy-supply systems involved. This paper intends to contribute for the establishment of parameters to measure the technical and economic viability for using such isolated hybrid systems, trying in the future several combinations of models to hybridize the energy generation in Amazon isolated villages, making easier the use of local energy resources. (author)

  6. Electric generation situation through hybrid systems in Para state and perspectives in face of the global supply of electric power; Situacao da geracao eletrica atraves de sistemas hibridos no estado do Para e perspectivas frente a universalizacao da energia eletrica

    Energy Technology Data Exchange (ETDEWEB)

    Barbosa, Claudomiro Fabio de Oliveira; Pinho, Joao Tavares; Pereira, Edinaldo Jose da Silva; Galhardo, Marcos Andre Barros; Vale, Silvio Bispo do; Maranhao, Wilson Monteiro de Albuquerque [Universidade Federal do Para (UFPA), Belem, PA (Brazil). Grupo de Estudos e Desenvolvimento de Alternativas Energeticas], e-mail: gedae@ufpa.br

    2004-07-01

    This work presents an analysis of the hybrid systems for electricity generation installed in the State of Para, emphasizing the profile of the supplied localities, the conversion technologies in the several configurations used for electric power generation, the social, economic and environmental impacts caused by such systems, the management and sustainability of the systems, and their perspectives in face of the global supply of electric power in Brazil. (author)

  7. Optimal design of a hybrid photovoltaic and fuel cell power generation system, to supply isolated communities in the Brazilian Amazon; Dimensionamento otimo de sistemas hibridos, com geracao fotovoltaica e celula a combustivel, para atendimento a comunidades isoladas na Amazonia

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Sergio Batista da

    2010-11-15

    The lack of electricity in isolated communities in the Brazilian Amazon has become one of the greatest barrier for the development of the region. Currently, the main technologies that provide electricity to these communities are diesel generators, batteries and dry cells. These non-renewable energy sources may pose serious problems to the environment and human health and have high maintenance and operational costs. Therefore, the search for renewable energy sources, such as water and sunlight, which are highly abundant in the region, has become a great challenge. This thesis presents the studies on application of solar photovoltaic (PV) and fuel cell (FC) technologies to supply electric power in an uninterrupted manner. Outlined are the technical and cost issues of a pilot project set up in an environmentally protected area, next to Bananal island, located in the Southwestern region of the state of Tocantins. The pilot project relies on PV solar power as the primary source of energy for the production of electricity. The surplus energy is stored in the form of hydrogen produced by electrolysis of the water supplied locally, which is reconverted into electric power by fuel cells during periods when there is little or no sunlight. In this context, the aim of the study was to propose a sizing of a hybrid distributed generation system (HDGS), comprised of a PV system, FC and batteries, that optimizes implementation and operational costs, as a potential source of energy for isolated communities in the Amazon. The work was carried out with the help of simulation software HOMER (Hybrid Optimization Model for Electric Renewable) developed by National Renewable Energy Laboratory (NREL). Simulations and a comparative study were carried out of the technologies and potential configurations that meet the needs of these isolated communities. The results showed an optimal solution of HGDS PV-FC batteries with a reduction in the initial cost of the project in about 60% compared to the sizing system only. The HOMER simulated results also indicated that more than 50% of the costs of the PV-FC-battery HDGS regarded the PV system. (author)

  8. Dimensioning of a photovoltaic-wind hybrid system in UFRJ campus of the Ilha do Fundao; Dimensionamento de um sistema hibrido eolico-fotovoltaico na UFRJ Campus Ilha do Fundao

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Silvio Carlos Anibal de [Universidade Federal do Rio de Janeiro (DEM/UFRJ), RJ (Brazil). Dept. de Engenharia Mecanica], e-mail: silvioa@gmail.com; Santos, Rodrigo Zimmer de Abreu; Argolo, Felipe Henrique Bohm; Danziger, Fernando Artur Ragoni; Sollero, Luiz Paulo Vervloet; Castro, Marcial Pereira Saboya de [Universidade Federal do Rio de Janeiro (DEM/UFRJ), RJ (Brazil). Dept. de Engenharia Industrial], e-mail: rodzimmer@gmail.com, e-mail: felipeargolo@yahoo.com.br, e-mail: ragonziger@gmail.com, e-mail: lpsollero@gmail.com, e-mail: marcial.saboya@gmail.com

    2008-07-01

    A techno-economic analysis of a grid connected photovoltaic-wind hybrid energy system is done in the present paper. The set-up consists of photovoltaic solar-cell arrays, wind generators, a lead acid storage batteries and an inverter unit to convert DC power to AC power. The study has been performed with the simulation tool HOMER (Hybrid Optimization Model for Electric Renewable) developed by the National Renewable Energy Laboratory (NREL) for techno-economical feasibility studies of hybrid systems. The system is connected to the electrical grid in order to supply all the electrical energy demand of the Technological Centre of the Federal University of Rio de Janeiro. Many systems configurations have been considered and it was possible to simulate the hourly operation of each system and to calculate technical, economic and environmental performance parameters. The best configuration is a Fuhrlander 30 wind generator, associated to a photovoltaic system to reach the generation of 96% of the energy demand by means of renewable sources. (author)

  9. BUTREN-RC an hybrid system for the recharges optimization of nuclear fuels in a BWR; BUTREN-RC un sistema hibrido para la optimizacion de recargas de combustible nuclear en un BWR

    Energy Technology Data Exchange (ETDEWEB)

    Ortiz S, J.J.; Castillo M, J.A. [ININ, Carretera Mexico-Toluca Km. 36.5, 52045 Estado de Mexico (Mexico); Valle G, E. del [IPN, ESFM, 07738 Mexico D.F. (Mexico)

    2004-07-01

    The obtained results with the hybrid system BUTREN-RC are presented that obtains recharges of nuclear fuel for a BWR type reactor. The system has implemented the methods of optimization heuristic taboo search and neural networks. The optimization it carried out with the technique of taboo search, and the neural networks, previously trained, were used to predict the behavior of the recharges of fuel, in substitution of commercial codes of reactor simulation. The obtained recharges of nuclear fuel correspond to 5 different operation cycles of the Laguna Verde Nuclear Power plant, Veracruz in Mexico. The obtained results were compared with the designs of this cycles. The energy gain with the recharges of fuel proposals is of approximately 4.5% with respect to those of design. The time of compute consumed it was considerably smaller that when a commercial code for reactor simulation is used. (Author)

  10. Study of a hybrid system using solid oxide fuel cells (SOFC) and gas turbine; Estudo de um sistema hibrido empregando celula de combustivel de oxido solido (SOFC) e turbina a gas

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Antonio Carlos Caetano de; Gallo, Giulliano Batelochi; Silveira, Jose Luz [UNESP, Guaratingueta, SP (Brazil). Faculdade de Engenharia. Dept. de Energia], e-mail: caetano@feg.unesp.br

    2004-07-01

    In this paper a hybrid solid oxide fuel cell (SOFC) system, applying a combined cycle using gas turbine for rational decentralized energy production is analyzed. The relative concepts about the fuel cell are presented, followed by some chemical and technical information such as the change of Gibbs free energy in isothermal fuel oxidation directly into electricity. This represents a very high fraction of the lower heating value (LHV) of a hydrocarbon fuel. In the next step a methodology for the study of SOFC and gas turbine system is developed, considering the electricity and steam production for a hospital. This methodology is applied to energetic analysis. Natural gas is considered as a fuel. A Sankey Diagram shows that the hybrid SOFC system is a good opportunity to strengthen the decentralized energy production in Brazil. It is necessary to consider that the cogeneration in this version also is a good technical alternative, demanding special methods of design, equipment selection and contractual deals associated to electricity and fuel supply. (author)

  11. The problem of the use the hybrid and photovoltaic systems in insular zones with climatic siversity in Madeira; A problematica da utilizacao de sistemas fotovoltaicos e hibridos em zonas insulares de diversidade climatica-ILHA da Madeira

    Energy Technology Data Exchange (ETDEWEB)

    Magro, J. C. E.

    2004-07-01

    Project PAUER contemplates the evaluation and utilization of renewable energy in insular zones with climatic diversity, situated on the Archipelago of Madeira. The project has three main areas of intervention: prospecting and evaluating energetic resources; creation of data bases of climatic data for application in energetic studies and other areas; the installation of twenty autonomous photovoltaic or hybrid systems in isolated localities. This communication presents some data from meteorological observations made up until the present date, following appropriate statistical analysis. Characteristics of some installed photovoltaic and hybrid systems as well as some of the principal problems encountered in the field are also given. (Author)

  12. Suggestion for a hybrid neuro genetic system as an alternative for the assessment of electric power consumption curves; Sugestao de um sistema hibrido neuro-genetico como alternativa a avaliacao de curvas de consumo de energia eletrica

    Energy Technology Data Exchange (ETDEWEB)

    Masselli, Yvo Marcelo Chiaradia; Torres, Germano Lambert [Universidade Federal de Itajuba (UNIFEI), MG (Brazil)]. E-mails: ymcm@unifei.edu.br; germano@unifei.edu.br

    2006-07-01

    Increase the electrical distribution network quality it's a permanent interesting of energy companies. Therefore, quality services with acceptable values must be offers. In direct form this involves to manage efficiently all distribution system. Nowadays, this supervision is carried out with Geographic Information System (GIS) tools associate with calculation of demands transformers, result of correlation curve kWh versus KV A. Simple linear regression is used to estimate this curve, which make the process not too accuracy. To estimate consumers loads, the traditional methodology consider only end consumption, refusing the characteristic of different kinds of consumers: residential, commercial, industrial, rural and others. This paper present a model based hybridism between Artificial Neural Network (ANN) and Genetic Algorithm (GA), that can identify on load curve acquired any point of distribution system, the portions of consumption relatives to each one of main consumers sectors in that point. Besides, is suggested to the same application an ANN type Multi-Layer Perceptron, with the purpose to compare the showing results and to verify the advantages of hybridism use. The Hybrid System proposed use GA to the ANN training, in other words, the determination of best values to the ANN synaptic weights. Definite the work methodology, the practical valuation of the model starts, and the results presented by hybrid system are compared with those generates by ANN training by the conventional method well-know Back propagation Error (BP). The practical application propose that load curve acquired been analyze in any point of distribution system. Knowing this curve, the system should identify instant consumption quantities, relative to each one of main consumers sectors: industrial, commercial and residential. Subsequently are consider oscillations about these curves, in way to approach the real situation. The initial result indicated a best performance of conventional ANN, however when the complexity training patterns increased, the hybrid system started to present best results, showing your power. Made the necessary simulations and checked the validity of association between ANN with GA, the developed system can be consider a powerful tool to the distribution electrical network management. (author)

  13. Software del sistema osteomioarticular

    Directory of Open Access Journals (Sweden)

    Dianelys León Medina

    2015-06-01

    Full Text Available Introducción: la aplicación en la enseñanza de las Tecnologías de la Informática y las Comunicaciones, es una de las líneas que ha trazado el sistema de salud cubano en estudiantes de las ciencias médicas. La anatomía es una de las ciencias que integra la disciplina de Morfofisiología y para facilitar su comprensión resulta necesario el empleo de recursos y estrategias, entre los que puede figurar un software educativo. Objetivo: diseñar un software sobre la anatomía del sistema osteomioarticular de cabeza y cuello para los estudiantes de primer año de la carrera de Estomatología de Pinar del Río. Material y método: se realizó un estudio cualitativo. Para obtener los resultados se utilizó el método materialista dialéctico. Para la dialéctica del desarrollo del proceso estudiado se utilizaron los métodos teóricos, empíricos, el estudio profundo del tema, tipo de plataforma a utilizar y criterios de los especialistas. Para su procesamiento se utilizaron técnicas estadísticas descriptivas e inferenciales no paramétricas. Resultados: debido a la necesidad de fortalecer en los estudiantes las habilidades en la utilización de las tecnologías de la informática y las comunicaciones, se elaboró el software de la anatomía del sistema osteomioarticular de cabeza y cuello "Aprendiendo anatomía" mediante imágenes, videos y textos. Conclusiones: el software constituye un aporte al proceso enseñanza aprendizaje, el cual facilita el trabajo independiente y autopreparación mediante la interactividad con el contenido, retroalimentación y evaluación utilizando medios de enseñanza modernos, lo cual tributa al modo de actuación del futuro egresado.

  14. Efficient refolding and immobilization of PMMA-tag-fused single-chain Fv antibodies for sensitive immunological detection on a PMMA plate.

    Science.gov (United States)

    Kumada, Yoichi; Ishikawa, Yasuyuki; Fujiwara, Yusuke; Takeda, Rui; Miyamoto, Ryosuke; Niwa, Daisuke; Momose, Shun; Kang, Bongmun; Kishimoto, Michimasa

    2014-09-01

    In this study, we investigated the efficient refolding and site-specific immobilization of single-chain variable fragments (scFvs) genetically fused with a poly(methylmethacrylate)-binding peptide (PMMA-tag). According to the results of an aggregation test of a scFv-PM in the presence of 0.5 M urea, aggregation was hardly detectable at a weak-alkaline pH (8.5) with lower concentrations of NaCl. Consequently, more than 93% recovery of the anti-RNase scFv-PM model was attained, when it was refolded by dialysis against 50 mM TAPS (pH8.5). These results suggested that the apparent isoelectric point (pI) of a target scFv was decreased to a great extent by the genetic fusion of a PMMA-tag containing 5 acidic amino acids, and, thus, the solubility of the scFv-PM in its semi-denatured form was considerably improved. We also designed alternative peptide-tags composed of plural aspartic acid residues (D5, D10 and D15-tags) to decrease the apparent pI value of the fusion protein. As a consequence, scFv-D5, scFv-D10 and scFv-D15 were also efficiently refolded with yields of more than 95%. It is noteworthy that even scFv-PS-D15, which had both a positively charged polystyrene-binding peptide (PS-tag) and a negatively charged D15-tag, was serially connected at the C-terminal region of scFvs, and also refolded with a yield of 96.1%. These results clearly indicate that controlling the apparent pI value of scFvs by the fusion of oligo-peptides composed of acidic amino acids at the C-terminus resulted in a high degree of recovery via dialysis refolding. According to the results of a sandwich ELISA using scFv-PMs, scFv-D15 and scFv-PS-D15 as ligands, high antigen-binding signals were detected from both the PMMA and phi-PS plates immobilized with scFv-PMs. Furthermore, the high antigen-binding activity of scFv-PMs was maintained in an adsorption state when it was immobilized on the surface of not only PMMA, but also hydrophilic PS (phi-PS) and polycarbonate (PC). These results

  15. El Sistema Universitario Ecuatoriano

    Directory of Open Access Journals (Sweden)

    Lilian Molina

    2016-12-01

    Full Text Available El presente trabajo muestra al sistema universitario ecuatoriano, con el fin de ayudar a entender el inicio de sus reformas en relación con la nueva Constitución y Ley Orgánica de Educación Superior, la importancia de la gratuidad en este nuevo escenario, así como también las nuevas tipologías y orden constitucional de creación de nuevas universidades para promover la investigación científica. Abstract  The present work shows the Ecuadorian university system, in order to help understand the beginning of its reforms in relation to the new Constitution and Organic Law of Higher Education, the importance of gratuitousness in this new scenario, as well as the new typologies And constitutional order of creation of new universities to promote scientific research.

  16. Diagnostic potential of recombinant scFv antibodies generated against hemagglutinin protein of influenza A virus

    Directory of Open Access Journals (Sweden)

    Roopali eRajput

    2015-09-01

    Full Text Available Human influenza A viruses have been the cause of enormous socio-economic losses worldwide. In order to combat such a notorious pathogen, hemagglutinin protein (HA has been a preferred target for generation of neutralizing-antibodies, as potent therapeutic/ diagnostic agents. In the present study, recombinant anti-HA single chain variable fragment (scFv antibodies were constructed using the phage display technology to aid in diagnosis and treatment of human influenza A virus infections. Spleen cells of mice hyper-immunized with A/New Caledonia/20/99 (H1N1 virus were used as the source for recombinant antibody (rAb production. The antigen-binding phages were quantified after 6 rounds of bio-panning against A/New Caledonia/20/99 (H1N1, A/California/07/2009 (H1N1-like, or A/Udorn/307/72(H3N2 viruses. The phage yield was maximum for the A/New Caledonia/20/99 (H1N1, however, considerable cross-reactivity was observed for the other virus strains as well. The HA-specific polyclonal rAb preparation was subjected to selection of single clones for identification of high reactive relatively conserved epitopes. The high affinity rAbs were tested against certain known conserved HA epitopes by peptide ELISA. Three recombinant mAbs showed reactivity with both the H1N1 strains and one (C5 showed binding with all the three viral strains. The C5 antibody was thus used for development of an ELISA test for diagnosis of influenza virus infection. Based on the sample size in the current analysis, the ELISA test demonstrated 83.9% sensitivity and 100% specificity. Thus, the ELISA, developed in our study, may prove as a cheaper alternative to the presently used real time RT-PCR test for detection of human influenza A viruses in clinical specimens, which will be beneficial, especially in the developing countries. Since, the two antibodies identified in this study are reactive to conserved HA epitopes; these may prove as potential therapeutic agents as well.

  17. Sistema Automatizado de Emailing Marketing

    OpenAIRE

    Martínez López, Javier

    2010-01-01

    Projecte final de carrera realitzat en col.laboració amb E-xpertel S.A El presente proyecto describe el diseño e implementación de un sistema de automatización de campañas de marketing a través de email hacia clientes y/o clientes potenciales. Este sistema tiene como objetivos principales adquirir nuevos clientes y conseguir la delización de los mismos. El sistema se complementa con una plataforma CRM (Customer Relationship Management) para el mantenimiento de datos de empresa...

  18. Avaliação Económica de um Sistema de Produção Fotovoltaico para Autoconsumo

    OpenAIRE

    Ana Lúcia Costa Gomes; António Pedro Soares Pinto; Joana Rita Silva Fialho

    2018-01-01

    A escassez de recursos naturais, o aumento de necessidades energéticas, os incentivos recentes em termos legislativos e uma preocupação crescente com as questões ambientais, desencadearam uma procura crescente de fontes alternativas de energia. Este estudo pretende analisar a viabilidade económica de um sistema fotovoltaico (FV) de produção de eletricidade para autoconsumo, no Campus Politécnico de Viseu. A avaliação foi efetuada, tendo por base a criação de 3 cenários para a potência de liga...

  19. Wheat cultivars selected for high Fv /Fm under heat stress maintain high photosynthesis, total chlorophyll, stomatal conductance, transpiration and dry matter.

    Science.gov (United States)

    Sharma, Dew Kumari; Andersen, Sven Bode; Ottosen, Carl-Otto; Rosenqvist, Eva

    2015-02-01

    The chlorophyll fluorescence parameter Fv /Fm reflects the maximum quantum efficiency of photosystem II (PSII) photochemistry and has been widely used for early stress detection in plants. Previously, we have used a three-tiered approach of phenotyping by Fv /Fm to identify naturally existing genetic variation for tolerance to severe heat stress (3 days at 40°C in controlled conditions) in wheat (Triticum aestivum L.). Here we investigated the performance of the previously selected cultivars (high and low group based on Fv /Fm value) in terms of growth and photosynthetic traits under moderate heat stress (1 week at 36/30°C day/night temperature in greenhouse) closer to natural heat waves in North-Western Europe. Dry matter accumulation after 7 days of heat stress was positively correlated to Fv /Fm . The high Fv /Fm group maintained significantly higher total chlorophyll and net photosynthetic rate (PN ) than the low group, accompanied by higher stomatal conductance (gs ), transpiration rate (E) and evaporative cooling of the leaf (ΔT). The difference in PN between the groups was not caused by differences in PSII capacity or gs as the variation in Fv /Fm and intracellular CO2 (Ci ) was non-significant under the given heat stress. This study validated that our three-tiered approach of phenotyping by Fv /Fm performed under increasing severity of heat was successful in identifying wheat cultivars differing in photosynthesis under moderate and agronomically more relevant heat stress. The identified cultivars may serve as a valuable resource for further studies to understand the physiological mechanisms underlying the genetic variability in heat sensitivity of photosynthesis. © 2014 Scandinavian Plant Physiology Society.

  20. Production of recombinant single chain antibodies (scFv) in vegetatively reproductive Kalanchoe pinnata by in planta transformation.

    Science.gov (United States)

    Jung, Yuchul; Rhee, Yong; Auh, Chung-Kyoon; Shim, Hyekyung; Choi, Jung-Jin; Kwon, Suk-Tae; Yang, Joo-Sung; Kim, Donggiun; Kwon, Myung-Hee; Kim, Yong-Sung; Lee, Sukchan

    2009-10-01

    We developed an asexual reproductive plant, Kalanchoe pinnata, as a new bioreactor for plant-based molecular farming using a newly developed transformation method. Leaf crenate margins were pin-pricked to infect the plant with the Agrobacterium strain LBA4404 and vacuum infiltration was also applied to introduce the target gene into the plants. Subsequently, the young mother leaf produced new clones at the leaf crenate margins without the need for time- and labor-consuming tissue culture procedures. The average transformation rates were approximately 77 and 84% for pin-prickling and vacuum-infiltration methods, respectively. To functionally characterize an introduced target protein, a nucleic acid hydrolyzing recombinant 3D8 scFv was selected and the plant based 3D8 scFv proteins were purified and analyzed. Based on abzyme analysis, the purified protein expressed with this system had catalytic activity and exhibited all of properties of the protein produced in an E. coli system. This result suggested that vegetatively reproductive K. pinnata can be a novel and potent bioreactor for bio-pharmaceutical proteins.

  1. Blastomicose do sistema nervoso

    Directory of Open Access Journals (Sweden)

    Horácio M. Canelas

    1951-09-01

    Full Text Available Os autores chamam a atenção sôbre os erros de diagnóstico nos casos de neuroblastomicose, seja nas formas meningoencefálicas, seja nas tumorais. São focalizadas a paracoccidioidose e a criptococose. Depois de caracterizarem clìnicamente a paracoccidioidose, acentuam a raridade das formas nervosas (1,2% dos casos dessa micose autopsiados no Departamento de Anatomia Patológica da Faculdade de Medicina da Universidade de São Paulo. Pela revisão da literatura foi verificado haver apenas 12 casos registrados. 0 caso de Casiello e Klass é o único em que o diagnóstico foi feito em vida; os demais, ou constituem achado de necropsia, ou foram inesperadamente revelados pelo exame histopatológico realizado após intervenções cirúrgicas. Os autores registram 2 casos de paracoccidioidose do sistema nervoso. No primeiro, tratava-se de meningomielorra-diculite crônica, sendo o parasito identificado no escarro; o segundo era portador de síndrome de compressão medular, cuja patogenia é discutida pelos autores, tendo sido a etiologia paracoccidióidica comprovada pelo exame da polpa e biópsia ganglionares. Considerações clínicas sôbre a criptococose precedem o relato de um caso de meningoencefalomielite subaguda, no qual fôra feito em vida o diagnóstico de paracoccidioidose, pelo exame micológico do liqüido cefalorraqueano; êste paciente, embora medicado com doses maciças de sulfa e tiossemicarbarsona, veio a falecer, tendo o exame anátomo-patológico revelado tratar-se, na realidade, de lesões nervosas produzidas pelo Crypíococcus neo-formans. Nos três casos era incisivo o caráter neurocirúrgico da sintomatologia. Entretanto, ante o diagnóstico de paracoccidioidose, foi instituído tratamento pelas sulfas, vacina específica e tiossemicarbarsona. Os resultados foram excelentes no caso 2, em que a terapêutica pôde ser instituída mais precocemente; melhoras também foram obtidas no caso 1. No caso 3, foram verificadas

  2. Micoses do sistema nervoso

    Directory of Open Access Journals (Sweden)

    Carlos da Silva Lacaz

    1947-03-01

    Full Text Available As micoses do sistema nervoso central não têm merecido atenção cuidadosa dos neurologistas. Elas afetam o neuraxe em diferente percentagem: a granulomatose criptocóccica (torulose é a mais freqüente, seguindo-se a actinomicose; a granulomatose paracoccidióidica só excepcionalmente determina lesões meningoencefálicas. Do ponto de vista clínico, as neuromicoses podem ser divididas em duas formas: tumorais encefalomedulares e meningíticas. Estas predominam sobre as tumorais, das quais são mais freqüentes as de localização encefálica. Não são raros os quadros mistos, meningoencetálicos. As formas tumorals encefálicas abrangem vários tipos anátomo-patológicos - abscesso, granuloma, nódulos e cistos - sendo mais comuns os dois primeiros, todos êles produzindo o quadro clinico da síndrome hipertensiva intracraniana. As formas meningomedulares, em geral, são secundárias às lesões ósseas vertebrais, sendo a actinomicose, por ser a mais osteófila, a micose que mais freqüentemente atinge a medula. Nestes casos, o quadro clínico da síndrome compressiva é o mais comum. As meninges participam do processo fúngico, na maior parte dos casos; as manifestações clínicas variam desde o simples meningismo, até as meningites purulentas e meningoencefalites graves, havendo a possibilidade de se formarem aracnoidites císticas, ependimites e bloqueios ventriculares, do aqueduto ou mesmo do canal raquidiano. O diagnóstico das micoses do sistema nervoso central é comumente um "achado de autópsia". Somente a verificação do cogumelo no líquor e o seu isolamento permitem um diagnóstico seguro. Outros dados liquóricos, quando bem interpretados com os achados clínicos e neurológicos, orientam o analista na pesquisa do agente fúngico. O quadro anátomo-patológico nada tem de caraterístico, a não ser a presença do agente parasitário e só o seu encontro firma o diagnóstico. Examinamos 25 casos de granulomatose

  3. Sistemas de tiempo real y sistemas distribuidos de tiempo real

    OpenAIRE

    Pesado, Patricia Mabel; Ramón, Hugo Dionisio; Boracchia, Marcos; Pasini, Ariel C.; De Vito, María Cecilia; Osella Massa, Germán Leandro; Estrebou, César Armando

    2006-01-01

    El proyecto de investigación y desarrollo en Sistemas de Software Distribuido, abarca aspectos que van desde los fundamentos del desarrollo (Técnicas de Ingeniería de Requerimientos, Metodologías de Gestión y Desarrollo de Proyectos, Técnicas de Planificación, Métricas, Normas de Calidad, Web-Systems) hasta la concepción de aplicaciones específicas orientadas a los procesos industriales (Sistemas de Planeamiento de Producción, Control Industrial en tiempo real), procesos de E-government (Voto...

  4. Prevalence of 1691G>A FV mutation in Poland compared with that in other Central, Eastern and South-Eastern European countries.

    Science.gov (United States)

    Adler, Grażyna; Clark, Jeremy S C; Loniewska, Beata; Czerska, Ewa; Salkic, Nermin N; Ciechanowicz, Andrzej

    2012-05-01

    The 1691G>A FV variant has been described as a common genetic risk factor in venous thromboembolism. The purpose of this study was to provide a further frequency value for 1691G>A FV in Poland and to collate summary data from Central (Poland, Czech, Slovakia), Eastern (Russia, Belarus, Ukraine) and South-Eastern (Slovenia, Croatia, Bosnia and Herzegovina, Serbia, Montenegro, Macedonia, Bulgaria) European countries. For this purpose in 2007 the 1691G>A FV variant was analyzed by polymerase chain reaction-restriction fragment length polymorphism from DNA collected in 2005-2006. We studied 650 subjects: 400 newborns and 250 older individuals (mean age 46.1 y) from Poland and compared results with reports from other countries, as well as with the frequency trend of 845G>A HFE across South-Eastern European countries using centroid cities. From our 1691G>A FV study we identified 626 GG homozygotes, 23 GA heterozygotes, and 1 AA homozygote (n = 650), giving an A allele frequency of 1.9%, and a summed frequency value for Poland of 2.0% (n = 1588); the frequency in Central European countries was 3.9% (n = 4559), mostly due to the high value in the Czech Republic: 5.1% (n = 2819); the South-Eastern European countries had 2.5% (n = 2410). Among the Eastern European countries the 1691G>A FV allele frequency was 1.9% (n=791), between the South-Eastern and Eastern European countries there was no significant difference (p=0.17). We confirm that the 1691G>A FV allele frequency in Poland, as well as other countries compared, is significantly lower than that in Czech.

  5. Identification of a GTP-bound Rho specific scFv molecular sensor by phage display selection

    Directory of Open Access Journals (Sweden)

    Chinestra Patrick

    2008-03-01

    Full Text Available Abstract Background The Rho GTPases A, B and C proteins, members of the Rho family whose activity is regulated by GDP/GTP cycling, function in many cellular pathways controlling proliferation and have recently been implicated in tumorigenesis. Although overexpression of Rho GTPases has been correlated with tumorigenesis, only their GTP-bound forms are able to activate the signalling pathways implicated in tumorigenesis. Thus, the focus of much recent research has been to identify biological tools capable of quantifying the level of cellular GTP-bound Rho, or determining the subcellular location of activation. However useful, these tools used to study the mechanism of Rho activation still have limitations. The aim of the present work was to employ phage display to identify a conformationally-specific single chain fragment variable (scFv that recognizes the active, GTP-bound, form of Rho GTPases and is able to discriminate it from the inactive, GDP-bound, Rho in endogenous settings. Results After five rounds of phage selection using a constitutively activated mutant of RhoB (RhoBQ63L, three scFvs (A8, C1 and D11 were selected for subsequent analysis. Further biochemical characterization was pursued for the single clone, C1, exhibiting an scFv structure. C1 was selective for the GTP-bound form of RhoA, RhoB, as well as RhoC, and failed to recognize GTP-loaded Rac1 or Cdc42, two other members of the Rho family. To enhance its production, soluble C1 was expressed in fusion with the N-terminal domain of phage protein pIII (scFv C1-N1N2, it appeared specifically associated with GTP-loaded recombinant RhoA and RhoB via immunoprecipitation, and endogenous activated Rho in HeLa cells as determined by immunofluorescence. Conclusion We identified an antibody, C1-N1N2, specific for the GTP-bound form of RhoB from a phage library, and confirmed its specificity towards GTP-bound RhoA and RhoC, as well as RhoB. The success of C1-N1N2 in discriminating activated

  6. Avaliação Económica de um Sistema de Produção Fotovoltaico para Autoconsumo

    Directory of Open Access Journals (Sweden)

    Ana Lúcia Costa Gomes

    2018-02-01

    Full Text Available A escassez de recursos naturais, o aumento de necessidades energéticas, os incentivos recentes em termos legislativos e uma preocupação crescente com as questões ambientais, desencadearam uma procura crescente de fontes alternativas de energia. Este estudo pretende analisar a viabilidade económica de um sistema fotovoltaico (FV de produção de eletricidade para autoconsumo, no Campus Politécnico de Viseu. A avaliação foi efetuada, tendo por base a criação de 3 cenários para a potência de ligação do sistema FV, com métricas tradicionais (valor atual líquido e paybacke complementada com o recurso a técnicas de opções reais (árvores de decisão, que permitem integrar, na avaliação, fontes de incerteza e flexibilidade operacional. Em termos globais, o investimento revela interesse económico e o payback é inversamente proporcional ao valor da tarifa suportada na aquisição de energia à rede.

  7. Analysis on the stress corrosion crack inception based on pit shape and size of the FV520B tensile specimen

    Science.gov (United States)

    Xiang, Longhao; Pan, Juyi; Chen, Songying

    2018-06-01

    The influence of pit shape and size on local stress concentration in the tensile specimen and the stress corrosion cracks inception was studied by employing the element remove technique. The maximum stress located in the bottom of pit on FV520B tensile specimen. The location of maximum strain was near the mouth of the pit or the shoulder and plastic strain existed in this region. Stress concentration factor and plastic deformation on four different geometrical shape pits of hemisphere, semi-ellipsoid, bullet and butterfly were numerically investigated, respectively. The simulation results showed that butterfly pit got the biggest stress concentration factor. The plastic strain rate during pit growth was in the sensitivity range of stress corrosion cracks inception, indicating that stress corrosion cracks were more likely to nucleate near the pit tip or the shoulder.

  8. Self-assembling complexes of quantum dots and scFv antibodies for cancer cell targeting and imaging.

    Directory of Open Access Journals (Sweden)

    Tatiana A Zdobnova

    Full Text Available Semiconductor quantum dots represent a novel class of fluorophores with unique physical and chemical properties which could enable a remarkable broadening of the current applications of fluorescent imaging and optical diagnostics. Complexes of quantum dots and antibodies are promising visualising agents for fluorescent detection of selective biomarkers overexpressed in tumor tissues. Here we describe the construction of self-assembling fluorescent complexes of quantum dots and anti-HER1 or anti-HER2/neu scFv antibodies and their interactions with cultured tumor cells. A binding strategy based on a very specific non-covalent interaction between two proteins, barnase and barstar, was used to connect quantum dots and the targeting antibodies. Such a strategy allows combining the targeting and visualization functions simply by varying the corresponding modules of the fluorescent complex.

  9. Preparation of the Fv fragment from a short-chain mouse IgG2a anti-dansyl monoclonal antibody and use of selectively deuterated Fv analogues for two-dimensional 1H NMR analyses fo the antigen-antibody interactions

    International Nuclear Information System (INIS)

    Takahashi, Hideo; Igarashi, Takako; Shimada, Ichio; Arata, Yoji

    1991-01-01

    The Fv fragment, a univalent antigen-binding unit with a molecular weight of 25,000, has successfully been prepared in high yield by limited proteolysis with clostripain of a short-chain mouse IgG2a anti-dansyl monoclonal antibody in which the entire C H 1 domain is deleted. The Fv fragment obtained is stable at room temperature and retains its full antigen-binding capability. It has been shown that selective deuterium labeling of the Fv fragment, which is half the size of the Fab fragment, provides 1 H NMR spectral data at a sufficient resolution for a detailed structural analysis of the antigen-combining site. NOESY spectra of an Fv analogue, in which all aromatic protons except for His C2'-H and Tyr C3',5'-H had been deuterated, were measured in the presence of varying amounts of dansyl-L-lysine. On the basis of the NOESY data obtained, it was possible to assign all the ring proton resonances for the dansly group that is bound to the Fv fragment. It was also possible to obtain information about His and Tyr residues of the Fv fragment in the absence and presence of the antigen. On the basis of the NMR data obtained, the authors have shown that at least two Tyr residues along with one of the amide groups are directly involved in antigen binding. The mode of interaction of the dansyl ring with these residues in the Fv fragment has briefly been discussed

  10. Preparation of the Fv fragment from a short-chain mouse IgG2a anti-dansyl monoclonal antibody and use of selectively deuterated Fv analogues for two-dimensional sup 1 H NMR analyses fo the antigen-antibody interactions

    Energy Technology Data Exchange (ETDEWEB)

    Takahashi, Hideo; Igarashi, Takako; Shimada, Ichio; Arata, Yoji (Univ. of Tokyo (Japan))

    1991-03-19

    The Fv fragment, a univalent antigen-binding unit with a molecular weight of 25,000, has successfully been prepared in high yield by limited proteolysis with clostripain of a short-chain mouse IgG2a anti-dansyl monoclonal antibody in which the entire C{sub H}1 domain is deleted. The Fv fragment obtained is stable at room temperature and retains its full antigen-binding capability. It has been shown that selective deuterium labeling of the Fv fragment, which is half the size of the Fab fragment, provides {sup 1}H NMR spectral data at a sufficient resolution for a detailed structural analysis of the antigen-combining site. NOESY spectra of an Fv analogue, in which all aromatic protons except for His C2{prime}-H and Tyr C3{prime},5{prime}-H had been deuterated, were measured in the presence of varying amounts of dansyl-L-lysine. On the basis of the NOESY data obtained, it was possible to assign all the ring proton resonances for the dansly group that is bound to the Fv fragment. It was also possible to obtain information about His and Tyr residues of the Fv fragment in the absence and presence of the antigen. On the basis of the NMR data obtained, the authors have shown that at least two Tyr residues along with one of the amide groups are directly involved in antigen binding. The mode of interaction of the dansyl ring with these residues in the Fv fragment has briefly been discussed.

  11. Thoughts on Seeing "El Sistema"

    Science.gov (United States)

    Booth, Eric

    2009-01-01

    The FESNOJIV (the Spanish acronym for the State Foundation for the National System of Youth and Children's Orchestras), more commonly known as "El Sistema," teaches 300,000 of Venezuela's poorest children in the nationwide music learning program. Many begin attending the "nucleo" as early as age two or three, and the vast…

  12. Knowledge Exchange with Sistema Scotland

    Science.gov (United States)

    Allan, Julie; Moran, Nikki; Duffy, Celia; Loening, Gica

    2010-01-01

    This paper reports on a knowledge exchange project, funded by the Scottish Funding Council and undertaken by a group of researchers from three higher education institutions in Scotland and the project partner, Sistema Scotland. This newly established charity is attempting to implement a major programme of social change, developed in Venezuela,…

  13. Exceptionally potent anti-tumor bystander activity of an scFv : sTRAIL fusion protein with specificity for EGP2 toward target antigen-negative tumor cells

    NARCIS (Netherlands)

    Bremer, E; Samplonius, D; Kroesen, BJ; van Genne, L; de Leij, L; Helfrich, W

    2004-01-01

    Previously, we reported on the target cell-restricted fratricide apoptotic activity of scFvC54:sTRAIL, a fusion protein comprising human-soluble tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) genetically linked to the antibody fragment scFvC54 specific for the cell surface target

  14. Affinity Purification of Tumor Necrosis Factor-α Expressed in Raji Cells by Produced scFv Antibody Coupled CNBr-Activated Sepharose

    Science.gov (United States)

    Abdolalizadeh, Jalal; Majidi Zolbanin, Jafar; Nouri, Mohammad; Baradaran, Behzad; Movassaghpour, AliAkbar; Farajnia, Safar; Omidi, Yadollah

    2013-01-01

    Purpose: Recombinant tumor necrosis factor-alpha (TNF-α) has been utilized as an antineoplastic agent for the treatment of patients with melanoma and sarcoma. It targets tumor cell antigens by impressing tumor-associated vessels. Protein purification with affinity chromatography has been widely used in the downstream processing of pharmaceutical-grade proteins. Methods:In this study, we examined the potential of our produced anti-TNF-α scFv fragments for purification of TNF-α produced by Raji cells. The Raji cells were induced by lipopolysaccharides (LPS) to express TNF-α. Western blotting and Fluorescence-activated cell sorting (FACS) flow cytometry analyses were used to evaluate the TNF-α expression. The anti-TNF-α scFv selected from antibody phage display library was coupled to CNBr-activated sepharose 4B beads used for affinity purification of expressed TNF-α and the purity of the protein was assessed by SDS-PAGE. Results: Western blot and FACS flow cytometry analyses showed the successful expression of TNF-α with Raji cells. SDS-PAGE analysis showed the performance of scFv for purification of TNF-α protein with purity over 95%. Conclusion: These findings confirm not only the potential of the produced scFv antibody fragments but also this highly pure recombinant TNF-α protein can be applied for various in vitro and in vivo applications. PMID:24312807

  15. DARPA Antibody Technology Program. Standardized Test Bed for Antibody Characterization: Characterization of an MS2 ScFv Antibody Produced by Illumina

    Science.gov (United States)

    2016-08-01

    ECBC-TR-1395 DARPA ANTIBODY TECHNOLOGY PROGRAM STANDARDIZED TEST BED FOR... ANTIBODY CHARACTERIZATION: CHARACTERIZATION OF AN MS2 SCFV ANTIBODY PRODUCED BY ILLUMINA Patricia E. Buckley Alena M. Calm Heather Welsh Roy...4. TITLE AND SUBTITLE DARPA Antibody Technology Program Standardized Test Bed for Antibody Characterization: Characterization of an MS2 ScFv

  16. Characterization of Fusobacterium varium Fv113-g1 isolated from a patient with ulcerative colitis based on complete genome sequence and transcriptome analysis.

    Directory of Open Access Journals (Sweden)

    Tsuyoshi Sekizuka

    Full Text Available Fusobacterium spp. present in the oral and gut flora is carcinogenic and is associated with the risk of pancreatic and colorectal cancers. Fusobacterium spp. is also implicated in a broad spectrum of human pathologies, including Crohn's disease and ulcerative colitis (UC. Here we report the complete genome sequence of Fusobacterium varium Fv113-g1 (genome size, 3.96 Mb isolated from a patient with UC. Comparative genome analyses totally suggested that Fv113-g1 is basically assigned as F. varium, in particular, it could be reclassified as notable F. varium subsp. similar to F. ulcerans because of partial shared orthologs. Compared with the genome sequences of F. varium ATCC 27725 (genome size, 3.30 Mb and other strains of Fusobacterium spp., Fv113-g1 possesses many accessary pan-genome sequences with noteworthy multiple virulence factors, including 44 autotransporters (type V secretion system, T5SS and 13 Fusobacterium adhesion (FadA paralogs involved in potential mucosal inflammation. Indeed, transcriptome analysis demonstrated that Fv113-g1-specific accessary genes, such as multiple T5SS and fadA paralogs, showed notably increased expression with D-MEM cultivation than with brain heart infusion broth. This implied that growth condition may enhance the expression of such potential virulence factors, leading to remarkable survival against other gut microorganisms and to the pathogenicity to human intestinal epithelium.

  17. Generation of anti-idiotype scFv for pharmacokinetic measurement in lymphoma patients treated with chimera anti-CD22 antibody SM03.

    Directory of Open Access Journals (Sweden)

    Qi Zhao

    Full Text Available Pre-clinical and clinical studies of therapeutic antibodies require highly specific reagents to examine their immune responses, bio-distributions, immunogenicity, and pharmacodynamics in patients. Selective antigen-mimicking anti-idiotype antibody facilitates the assessment of therapeutic antibody in the detection, quantitation and characterization of antibody immune responses. Using mouse specific degenerate primer pairs and splenocytic RNA, we generated an idiotype antibody-immunized phage-displayed scFv library in which an anti-idiotype antibody against the therapeutic chimera anti-CD22 antibody SM03 was isolated. The anti-idiotype scFv recognized the idiotype of anti-CD22 antibody and inhibited binding of SM03 to CD22 on Raji cell surface. The anti-idiotype scFv was subsequently classified as Ab2γ type. Moreover, our results also demonstrated firstly that the anti-idiotype scFv could be used for pharmacokinetic measurement of circulating residual antibody in lymphoma patients treated with chimera anti-CD22 monoclonal antibody SM03. Of important, the present approach could be easily adopted to generate anti-idiotype antibodies for therapeutic antibodies targeting membrane proteins, saving the cost and time for producing a soluble antigen.

  18. Wheat cultivars selected for high Fv/Fm under heat stress maintain high photosynthesis, total chlorophyll, stomatal conductance, transpiration and dry matter

    DEFF Research Database (Denmark)

    Sharma, Dew Kumari; Andersen, Sven Bode; Ottosen, Carl Otto

    2015-01-01

    ) than the low group, accompanied by higher stomatal conductance (gs), transpiration rate (E) and evaporative cooling of the leaf (ΔT). The difference in PN between the groups was not caused by differences in PSII capacity or gs as the variation in Fv/Fm and intracellular CO2 (Ci) was non...

  19. FvBck1, a Component of Cell Wall Integrity MAP Kinase Pathway, is Required for Virulence and Oxidative Stress Response in Sugarcane Pokkah Boeng Pathogen

    Directory of Open Access Journals (Sweden)

    Chengkang eZhang

    2015-10-01

    Full Text Available Fusarium verticillioides (formerly F. moniliforme is suggested as one of the causal agents of Pokkah Boeng, a serious disease of sugarcane worldwide. Currently, detailed molecular and physiological mechanism of pathogenesis is unknown. In this study, we focused on cell wall integrity MAPK pathway as one of the potential signaling mechanisms associated with Pokkah Boeng pathogenesis. We identified FvBCK1 gene that encodes a MAP kinase kinase kinase homolog and determined that it is not only required for growth, micro- and macro-conidia production, and cell wall integrity but also for response to osmotic and oxidative stresses. The deletion of FvBCK1 caused a significant reduction in virulence and FB1 production, a carcinogenic mycotoxin produced by the fungus. Moreover, we found the expression levels of three genes, which are known to be involved in superoxide scavenging, were down regulated in the mutant. We hypothesized that the loss of superoxide scavenging capacity was one of the reasons for reduced virulence, but overexpression of catalase or peroxidase gene failed to restore the virulence defect in the deletion mutant. When we introduced Magnaporthe oryzae MCK1 into the FvBck1 deletion mutant, while certain phenotypes were restored, the complemented strain failed to gain full virulence. In summary, FvBck1 plays a diverse role in F. verticillioides, and detailed investigation of downstream signaling pathways will lead to a better understanding of how this MAPK pathway regulates Pokkah Boeng on sugarcane.

  20. Improved scFv Anti-HIV-1 p17 Binding Affinity Guided from the Theoretical Calculation of Pairwise Decomposition Energies and Computational Alanine Scanning

    Directory of Open Access Journals (Sweden)

    Panthip Tue-ngeun

    2013-01-01

    Full Text Available Computational approaches have been used to evaluate and define important residues for protein-protein interactions, especially antigen-antibody complexes. In our previous study, pairwise decomposition of residue interaction energies of single chain Fv with HIV-1 p17 epitope variants has indicated the key specific residues in the complementary determining regions (CDRs of scFv anti-p17. In this present investigation in order to determine whether a specific side chain group of residue in CDRs plays an important role in bioactivity, computational alanine scanning has been applied. Molecular dynamics simulations were done with several complexes of original scFv anti-p17 and scFv anti-p17mutants with HIV-1 p17 epitope variants with a production run up to 10 ns. With the combination of pairwise decomposition residue interaction and alanine scanning calculations, the point mutation has been initially selected at the position MET100 to improve the residue binding affinity. The calculated docking interaction energy between a single mutation from methionine to either arginine or glycine has shown the improved binding affinity, contributed from the electrostatic interaction with the negative favorably interaction energy, compared to the wild type. Theoretical calculations agreed well with the results from the peptide ELISA results.

  1. Affinity Purification of Tumor Necrosis Factor-α Expressed in Raji Cells by Produced scFv Antibody Coupled CNBr-Activated Sepharose

    Directory of Open Access Journals (Sweden)

    Safar Farajnia

    2013-02-01

    Full Text Available Purpose: Recombinant tumor necrosis factor-alpha (TNF-α has been utilized as an antineoplastic agent for the treatment of patients with melanoma and sarcoma. It targets tumor cell antigens by impressing tumor-associated vessels. Protein purification with affinity chromatography has been widely used in the downstream processing of pharmaceutical-grade proteins. Methods: In this study, we examined the potential of our produced anti-TNF-scFv fragments for purification of TNF-α produced by Raji cells. he Raji cells were induced by lipopolysaccharides (LPS to express TNF-α. Western blotting and Fluorescence-activated cell sorting (FACS flow cytometry analyses were used to evaluate the TNF-α expression. The anti-TNF-α scFv selected from antibody phage display library was coupled to CNBr-activated sepharose 4B beads used for affinity purification of expressed TNF-α and the purity of the protein was assessed by SDS-PAGE. Results: Western blot and FACS flow cytometry analyses showed the successful expression of TNF-α with Raji cells. SDS-PAGE analysis showed the performance of scFv for purification of TNF-α protein with purity over 95%. Conclusion: These findings confirm not only the potential of the produced scFv antibody fragments but also this highly pure recombinant TNF-α protein can be applied for various in vitro and in vivo applications.

  2. Glycosylphosphatidylinositol-Anchored Anti-HIV scFv Efficiently Protects CD4 T Cells from HIV-1 Infection and Deletion in hu-PBL Mice

    Science.gov (United States)

    Ye, Chaobaihui; Wang, Weiming; Cheng, Liang; Li, Guangming; Wen, Michael; Wang, Qi; Zhang, Qing; Li, Dan

    2016-01-01

    ABSTRACT Despite success in viral inhibition and CD4 T cell recovery by highly active antiretroviral treatment (HAART), HIV-1 is still not curable due to the persistence of the HIV-1 reservoir during treatment. One patient with acute myeloid leukemia who received allogeneic hematopoietic stem cell transplantation from a homozygous CCR5 Δ32 donor has had no detectable viremia for 9 years after HAART cessation. This case has inspired a field of HIV-1 cure research focusing on engineering HIV-1 resistance in permissive cells. Here, we employed a glycosylphosphatidylinositol (GPI)-scFv X5 approach to confer resistance of human primary CD4 T cells to HIV-1. We showed that primary CD4 T cells expressing GPI-scFv X5 were resistant to CCR5 (R5)-, CXCR4 (X4)-, and dual-tropic HIV-1 and had a survival advantage compared to control cells ex vivo. In a hu-PBL mouse study, GPI-scFv X5-transduced CD4 T cells were selected in peripheral blood and lymphoid tissues upon HIV-1 infection. Finally, GPI-scFv X5-transduced CD4 T cells, after being cotransfused with HIV-infected cells, showed significantly reduced viral loads and viral RNA copy numbers relative to CD4 cells in hu-PBL mice compared to mice with GPI-scFv AB65-transduced CD4 T cells. We conclude that GPI-scFv X5-modified CD4 T cells could potentially be used as a genetic intervention against both R5- and X4-tropic HIV-1 infections. IMPORTANCE Blocking of HIV-1 entry is one of most promising approaches for therapy. Genetic disruption of the HIV-1 coreceptor CCR5 by nucleases in T cells is under 2 clinical trials and leads to reduced viremia in patients. However, the emergence of viruses using the CXCR4 coreceptor is a concern for therapies applying single-coreceptor disruption. Here, we report that HIV-1-permissive CD4 T cells engineered with GPI-scFv X5 are resistant to R5-, X4-, or dual-tropic virus infection ex vivo. In a preclinical study using hu-PBL mice, we show that CD4 T cells were protected and that GPI-scFv X5

  3. Introducción al estudio de los sistemas económicos

    OpenAIRE

    García Nossa, Antonio

    2010-01-01

    Interdependencia de los sistemas; Coexistencia de los sistemas; Sucesión y coexistencia; Sistemas históricos y sistemas geopolíticos; Factores cuantitativos y cualitativos de los sistemas; Principios generales de los sistemas.

  4. Potent inhibition of OKT3-induced T cell proliferation and suppression of CD147 cell surface expression in HeLa cells by scFv-M6-1B9.

    Science.gov (United States)

    Intasai, Nutjeera; Tragoolpua, Khajornsak; Pingmuang, Prakitnavin; Khunkaewla, Panida; Moonsom, Seangdeun; Kasinrerk, Watchara; Lieber, André; Tayapiwatana, Chatchai

    2008-01-01

    CD147, a multifunctional type I transmembrane glycoprotein, has been implicated in various physiological and pathological processes. It is involved in signal transduction pathways and also plays a crucial role in the invasive and metastatic activity of malignant tumor cells. Diminished expression of this molecule has been shown to be beneficial in suppression of tumor progression. In a previous study, we generated and characterized a recombinant antibody fragment, scFv, which reacted specifically to CD147. In the present study, we further investigated the biological properties, function and the effect of generated scFv on CD147 expression. The in vitro study showed that soluble scFv-M6-1B9 produced from E. coli HB2151 bound to CD147 surface molecule and inhibited OKT3-induced T cell proliferation. Furthermore, soluble lysate of scFv-M6-1B9 from 293A cells, transduced with a scFv-M6-1B9 expressing adenovirus vector, recognized both recombinant and native CD147. These results indicate that scFv-M6-1B9 binds with high efficiency and specificity. Importantly, scFv-M6-1B9 intrabody reduced the expression of CD147 on the cell surface of HeLa cells suggesting that scFv-M6-1B9 is biologically active. In conclusion, our present study demonstrated that scFv-M6-1B9 has a great potential to target both the intracellular and the extracellular CD147. The generated scFv-M6-1B9 may be an effective agent to clarify the cellular function of CD147 and may aid in efforts to develop a novel treatment in various human carcinomas.

  5. Isolation and characterisation of a human-like antibody fragment (scFv that inactivates VEEV in vitro and in vivo.

    Directory of Open Access Journals (Sweden)

    Torsten Rülker

    Full Text Available Venezuelan equine encephalitis virus (VEEV belongs to the Alphavirus genus and several species of this family are pathogenic to humans. The viruses are classified as potential agents of biological warfare and terrorism and sensitive detection as well as effective prophylaxis and antiviral therapies are required.In this work, we describe the isolation of the anti-VEEV single chain Fragment variable (scFv, ToR67-3B4, from a non-human primate (NHP antibody gene library. We report its recloning into the bivalent scFv-Fc format and further immunological and biochemical characterisation.The scFv-Fc ToR67-3B4 recognised viable as well as formalin and ß-propionolactone (ß-Pl inactivated virus particles and could be applied for immunoblot analysis of VEEV proteins and immuno-histochemistry of VEEV infected cells. It detected specifically the viral E1 envelope protein of VEEV but did not react with reduced viral glycoprotein preparations suggesting that recognition depends upon conformational epitopes. The recombinant antibody was able to detect multiple VEEV subtypes and displayed only marginal cross-reactivity to other Alphavirus species except for EEEV. In addition, the scFv-Fc fusion described here might be of therapeutic use since it successfully inactivated VEEV in a murine disease model. When the recombinant antibody was administered 6 hours post challenge, 80% to 100% of mice survived lethal VEEV IA/B or IE infection. Forty to sixty percent of mice survived when scFv-Fc ToR67-3B4 was applied 6 hours post challenge with VEEV subtypes II and former IIIA. In combination with E2-neutralising antibodies the NHP antibody isolated here could significantly improve passive protection as well as generic therapy of VEE.

  6. Construction, expression, and function of 6B11ScFv-mIL-12, a fusion protein that attacks human ovarian carcinoma.

    Science.gov (United States)

    Cheng, Hongyan; Ye, Xue; Chang, Xiaohong; Ma, Ruiqiong; Cong, Xu; Niu, Yidong; Zhang, Menglei; Liu, Kai; Cui, Heng; Sang, Jianli

    2015-04-01

    We previously produced an anti-idiotypic monoclonal antibody, 6B11, which mimics ovarian cancer antigen CA166-9 and induces cellular and humoral immunity. Here, to enhance the immunogenicity of 6B11, we constructed the 6B11ScFv-mIL-12 fusion protein (FP), by fusing single-chain fragment of 6B11 variable region (6B11ScFv) with mouse interleukin-12 (mIL-12), which was expressed in eukaryotic 293EBNA cells transfected with pSBI vectors. A binding activity assay showed 6B11ScFv-mIL-12 to have activities of both 6B11 and mIL-12-it specifically bound both ovarian monoclonal antibody COC166-9 and rabbit anti-mouse IL-12 antibody. The immune activity assay showed 6B11ScFv-mIL-12 to promote proliferation of lymphocytes stimulated by phytohemagglutinin, increase the absolute numbers and percentages of CD3(-)/CD56(+) natural killer cells and CD3(+)/CD56(+) natural killer T cells among peripheral lymphocytes, and increase interferon-γ. The FP was specifically cytotoxic to the CA166-9(+) ovarian cancer cell lines HOC1A and SKOV3 and inhibited growth of ID8 subcutaneous tumors in C57BL/6J mice. This study provides an experimental basis for clinical use of 6B11ScFv-mIL-12 in ovarian cancer therapy. To our knowledge, this is the first report of a fusion protein from an anti-idiotypic antibody and IL-12.

  7. Estimación del área y del peso seco foliar en Elaeis guineellsis, Elaeis oleifera y el hibrido interespecifico E. Guineensis x E. Oleífera

    Directory of Open Access Journals (Sweden)

    Contreras Angela P.

    1999-12-01

    Full Text Available

    Este trabajo se planteo can la finalidad de determinar modelos estadísticos que permitan estimar el área y el peso foliar a través de métodos indirectos (no destructivos en Elaeis guineensis (Palma africana, Elaeis oleifera (Palma Noli, y el hibrido interespecifico E. guineensis X E. oleifera (Afrieana X Noli . Los experimentos de campo se efectuaron en las Haciendas Santa Bárbara y Chaparral-Cuernavaca, de la plantación Unipalma, ubicadas en la zona palmera de los llanos orientales en Colombia. Como resultado de la investigación, se obtuvo la validación del modelo propuesto por Corley et a1. (1971 y la innovación y ajuste de nuevos modelos que estiman los parámetros de crecimiento sin necesidad de muestreos destructivos. Los modelos propuestos en este trabajo, están ajustados a las condiciones del trópico colombiano.

     

    Palabras claves: Palma de aceite, área foliar, peso foliar, parámetros de crecimiento.

  8. Design and construction of a go-kart hybrid PEM fuel cell / rechargeable battery; Diseno y construccion de un go-kart hibrido pila de combustible PEM / bateria recargable

    Energy Technology Data Exchange (ETDEWEB)

    Suarez Alcantara, Karina; Rodriguez Castellanos, Andres; Soloza Feria, Omar [Centro de Investigacion y de Estudios Avanzados del IPN, Mexico D.F. (Mexico)]. E-mail: k.suarez.alcantara@gmail.com

    2008-11-15

    An hybrid Polymer Electrolyte Membrane Fuel Cell, PEMFC-Rechargeable Battery Go-kart has been designed and manufactured using AutoCAD software for the design and a CNC mechanical machine for the manufacture of components of the fuel cell. The membrane-electrode assemblies, MEAs, were integrated with a Gore-Select membrane and carbon cloth with Pt (20 wt % /C) 0.5 mg/cm{sup 2} anode and cathode electrode catalysts loading. High density graphite collector plates with 5mm thickness were used as collector plates. The estimated weigh of the go-kart with a driver is about 120 kg. The demand of the motor of the go-kart is 20 V and 5 A (100W), supplied by an hybrid system integrated by three 30Watts PEMFC. The commercially available Pb/acid rechargeable battery supplies energy for peripheral equipment. [Spanish] En este trabajo se presenta el diseno y la construccion de un go-kart hibrido pila de combustible con membrana de conduccion protonica tipo PEM (Proton Exchange Membrane, por sus siglas en ingles) y pila recargable. El diseno de los colectores de corriente de la pila se realizo utilizando el programa AutoCAD y la construccion mediante una fresadora con control numerico, CNC. Los ensambles membrana-electrocatalizador de la pila estan formados por membranas Gore-Select y por electrodos de Pt soportado en tela de carbon al 20 %peso/C con carga de 0.5 mg /cm{sup 2}, en anodo y catodo. Los platos colectores de corriente fueron manufacturados en grafito de alta densidad con espesor de 5 mm. La caracterizacion de la pila de combustible se realizo mediante ensayos de polarizacion potenciostatica. El peso total del go-kart y una persona a bordo es de 120 kg. La potencia del go-kart es generada por un motor de corriente directa de 20 V y 5 A (100 Watts). Para tal efecto, se construyeron tres pilas de combustible de 30 W cada una, con un respaldo de baterias recargables comerciales de Pb/acido para energizar equipos perifericos.

  9. La escoba de bruja del cacao [Crinipellis perniciosa (Stahel Singer] en la región del Piedemonte Llanero de Colombia : estudio de parámetros de resistencia en hibridos comerciales y clones de cacao

    Directory of Open Access Journals (Sweden)

    Tovar Germán

    1991-06-01

    Full Text Available Se estimaron algunos parámetros para la evaluación de la resistencia del cacao a C.perniciosa y se evaluó la respuesta a la infección de un grupo de híbridos comerciales y sus respectivos clones parentales. El grupo de híbridos inoculados en la radícula mostró, en general, tasas y porcentajes de infección importantes y no se encontraron diferencias significativas entre híbridos para las variables longitud de raicillas, altura de plántulas, diámetro del nudo cotiledonar y la relación entre la base del hipocótilo y el diámetro del nudo cotiledonar. De las cuatro variables evaluadas, la última es la que tiene una relación más estrecha con la susceptibilidad a nivel histológico, las otras variables son en  cierta medida dependientes de ésta y/o del vigor de cada hibrido como en el caso de la altura. La inoculación de los híbridos en la yerna terminal mostró un alto porcentaje de plántulas infectadas (65 a 85% y las tasas de infección fueron significativamente más altas (0,68 que en las inoculaciones en la radícula (0,27. Sin embargo, no todas las infecciones de 1averna terminal formaron escobas típicas (53,7%, sino que un porcentaje notable (36,2% correspondió a escobas látigo y un 16% a hipertrofias localizadas. Las pruebas sobre clones dieron respuestas ampliamente diferentes para el porcentaje de plántulas infectadas y el nivel de la respuesta fue menor, lo que indica una menor susceptibilidad. Algunos clones solo desarrollaron escobas látigo no esporulantes mientras que otros mostraron un bajo porcentaje de escobas típicas. Por consiguiente, los clones tienen una ventaja comparativa importante en relación con los híbridos en lo que concierne a la resistencia a escoba de bruja.

  10. Sistema Kaizen en la administracion

    Directory of Open Access Journals (Sweden)

    Gallegos, H.

    2007-01-01

    Full Text Available En este artículo presentaremos la importancia que tiene el área de administración como factor de reducción de costos para la empresa aplicando el sistema kaizen. Analizaremos en forma general la administración de las empresas, enfocándonos en las áreas de oportunidad que pueden existir, así como la posible solución a esto, utilizando el sistema kaizen, obteniendo como resultado una reducción de costos, tiempos o mejoras en los procesos administrativos. También se anexa un caso practico en el cual se muestra como aplicando la metodología kaizen en las actividades administrativas podemos lograr una reducción de tiempos y/o costos todo esto causado por la reingeniería de los procesos.

  11. Sistemas de control en estructuras

    Directory of Open Access Journals (Sweden)

    José Luis Ahumada Villafañe

    2010-01-01

    Full Text Available En el diseño estructural, la capacidad de disipación de energía en elementos que conforman los sistemas resistentes a fuerzas dinámicas, como las impartidas por la actividad sísmica y eólica, es suministrada por un minucioso detallado del acero de refuerzo y dimensiones en las secciones transversales de los elementos. Esta práctica es realizada bajo muchas suposiciones que en algunas ocasiones no corresponden a la realidad, y como es de esperarse, producen resultados inexactos y un desconocimiento del desempeño de la estructura. Por lo anterior es riesgoso confiar el 100% de la capacidad estructural a los elementos sobre todo cuando conforman sistemas estructurales diseñados en zonas de amenaza sísmica alta. En este artículo se presentan los sistemas de control, los cuales son una serie de dispositivos adaptados a las estructuras que absorben gran parte de la energía sísmica y liberan los elementos estructurales en gran porcentaje de la acción sísmica, logrando disminuir en ellas las solicitaciones (fuerzas internas y las respuestas (derivas con el objetivo de conseguir un buen desempeño, limitar el daño y abaratar los costos de reparación de fallas localizadas. Los sistemas de control que se analizarán en el presente artículo se dividen en control pasivo, control activo, control semiactivo y control híbrido.

  12. El sistema reticulo-endotelial

    Directory of Open Access Journals (Sweden)

    Carlos J. Cuartas L.

    1935-01-01

    multicelulares de organización bastante complicada, necesitan de mecanismos biológicos diferenciados para su defensa contra los agentes endógenos y exógenos, es asimismo razonable admitir en los grupos zoológicos inferiores, la existencia de constituyentes mesenquimáticos, que indican "una mayor amplitud para los límites que alcanza la representación del sistema dentro de la filogénesis".

  13. Development of a Targeted anti-HER2 scFv Chimeric Peptide for Gene Delivery into HER2-Positive Breast Cancer Cells.

    Science.gov (United States)

    Cheraghi, Roya; Nazari, Mahboobeh; Alipour, Mohsen; Majidi, Asia; Hosseinkhani, Saman

    2016-12-30

    Chimeric polymers are known as suitable carriers for gene delivery. Certain properties are critical for a polymer to be used as a gene delivery vector. A new polymer was designed for the targeted delivery of genes into breast cancer cell lines, based on MPG peptide. It is composed of different functional domains, including HIV gp41, nuclear localization sequence of SV40 T-antigen, two C-terminus repeats of histone H1, and the scFv of anti-HER2 antibody. The results demonstrated that the vector can effectively condense plasmid DNA into nanoparticles with an average size of 250nm. Moreover, fusion of the scFv portion to the carrier brought about the specific recognition of HER2. Overall, the transfection efficiency of the vector demonstrated that it could deliver the desired gene into BT-474 HER2-positive breast cancer cells. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. Ophiophagus hannah Venom: Proteome, Components Bound by Naja kaouthia Antivenin and Neutralization by N. kaouthia Neurotoxin-Specific Human ScFv

    Directory of Open Access Journals (Sweden)

    Witchuda Danpaiboon

    2014-05-01

    Full Text Available Venomous snakebites are an important health problem in tropical and subtropical countries. King cobra (Ophiophagus hannah is the largest venomous snake found in South and Southeast Asia. In this study, the O. hannah venom proteome and the venom components cross-reactive to N. kaouthia monospecific antivenin were studied. O. hannah venom consisted of 14 different protein families, including three finger toxins, phospholipases, cysteine-rich secretory proteins, cobra venom factor, muscarinic toxin, L-amino acid oxidase, hypothetical proteins, low cysteine protein, phosphodiesterase, proteases, vespryn toxin, Kunitz, growth factor activators and others (coagulation factor, endonuclease, 5’-nucleotidase. N. kaouthia antivenin recognized several functionally different O. hannah venom proteins and mediated paratherapeutic efficacy by rescuing the O. hannah envenomed mice from lethality. An engineered human ScFv specific to N. kaouthia long neurotoxin (NkLN-HuScFv cross-neutralized the O. hannah venom and extricated the O. hannah envenomed mice from death in a dose escalation manner. Homology modeling and molecular docking revealed that NkLN-HuScFv interacted with residues in loops 2 and 3 of the neurotoxins of both snake species, which are important for neuronal acetylcholine receptor binding. The data of this study are useful for snakebite treatment when and where the polyspecific antivenin is not available. Because the supply of horse-derived antivenin is limited and the preparation may cause some adverse effects in recipients, a cocktail of recombinant human ScFvs for various toxic venom components shared by different venomous snakes, exemplified by the in vitro produced NkLN-HuScFv in this study, should contribute to a possible future route for an improved alternative to the antivenins.

  15. Ophiophagus hannah venom: proteome, components bound by Naja kaouthia antivenin and neutralization by N. kaouthia neurotoxin-specific human ScFv.

    Science.gov (United States)

    Danpaiboon, Witchuda; Reamtong, Onrapak; Sookrung, Nitat; Seesuay, Watee; Sakolvaree, Yuwaporn; Thanongsaksrikul, Jeeraphong; Dong-din-on, Fonthip; Srimanote, Potjanee; Thueng-in, Kanyarat; Chaicumpa, Wanpen

    2014-05-13

    Venomous snakebites are an important health problem in tropical and subtropical countries. King cobra (Ophiophagus hannah) is the largest venomous snake found in South and Southeast Asia. In this study, the O. hannah venom proteome and the venom components cross-reactive to N. kaouthia monospecific antivenin were studied. O. hannah venom consisted of 14 different protein families, including three finger toxins, phospholipases, cysteine-rich secretory proteins, cobra venom factor, muscarinic toxin, L-amino acid oxidase, hypothetical proteins, low cysteine protein, phosphodiesterase, proteases, vespryn toxin, Kunitz, growth factor activators and others (coagulation factor, endonuclease, 5'-nucleotidase). N. kaouthia antivenin recognized several functionally different O. hannah venom proteins and mediated paratherapeutic efficacy by rescuing the O. hannah envenomed mice from lethality. An engineered human ScFv specific to N. kaouthia long neurotoxin (NkLN-HuScFv) cross-neutralized the O. hannah venom and extricated the O. hannah envenomed mice from death in a dose escalation manner. Homology modeling and molecular docking revealed that NkLN-HuScFv interacted with residues in loops 2 and 3 of the neurotoxins of both snake species, which are important for neuronal acetylcholine receptor binding. The data of this study are useful for snakebite treatment when and where the polyspecific antivenin is not available. Because the supply of horse-derived antivenin is limited and the preparation may cause some adverse effects in recipients, a cocktail of recombinant human ScFvs for various toxic venom components shared by different venomous snakes, exemplified by the in vitro produced NkLN-HuScFv in this study, should contribute to a possible future route for an improved alternative to the antivenins.

  16. Reflexiones sobre los sistemas silvopastoriles

    Directory of Open Access Journals (Sweden)

    R. O Russo

    Full Text Available El objetivo de este análisis es reflexionar acerca de modelos integrados de producción ganadera familiar y extensiva, más estables y sustentables, en los que se integre el componente leñoso, que contribuye a la reducción de gases con efecto invernadero y favorece la mitigación del cambio climático. Para ello se hizo una revisión de conceptos referentes a la integración de la actividad forestal en la ganadería, como alternativa viable de sistema de producción. Se parte del criterio de que los sistemas silvopastoriles (SSP, dentro de los agroforestales, son agroecosistemas en los que se asocia un componente arbóreo con uno herbáceo (pasturas naturales o mejoradas y otro pecuario (ganado en un mismo sitio, donde existen interacciones biológicas entre estos y se maximiza el uso de la tierra. También se describe cómo se agrupan los SSP; sus oportunidades desde los puntos de vista económico, productivo, social y ambiental; y los efectos de la interacción entre sus componentes. Este análisis permite plantear que los SSP son producto de la relación entre la biología, la sociedad y la cultura, y en ellos existe una enorme diversidad; asimismo, permiten la reconversión de la ganadería extensiva de muy baja productividad en sistemas más productivos y sostenibles en el tiempo, así como la rehabilitación de las áreas degradadas por ese tipo de ganadería, por la deforestación y por el agotamiento de los suelos.

  17. Isolation of scFv antibody fragments against HER2 and CEA tumor antigens from combinatorial antibody libraries derived from cancer patients.

    Science.gov (United States)

    Ayat, Hoda; Burrone, Oscar R; Sadghizadeh, Majid; Jahanzad, Eissa; Rastgou, Nasrin; Moghadasi, Sarrira; Arbabi, Mehdi

    2013-11-01

    Tumor cells expressing HER-2/neu and CEA antigens are potentially ideal targets for antibody-targeted therapy. In this study, two large human combinatorial libraries have been generated from the lymph nodes of breast cancer patients that express HER2 and CEA antigens in their tumors. These 'immune' libraries have been constructed in two different formats of scFv, differing in the length of the peptide linker connecting the two variable VH and VL domains. Libraries derived from these patients may contain a larger pool of anti-tumor antigen antibodies and are useful repertoire for isolating scFvs against any tumor markers. The results of this study showed that we were successful in obtaining human scFvs against HER-2/neu and CEA. For HER-2, cell-panning strategy was performed and resulted in two scFv binders that detected the complete HER-2 receptor on the cell membrane and internalized to the cells. Also, preliminary ELISA data showed that several anti-CEA scFv binders were isolated by panning. Copyright © 2013 The International Alliance for Biological Standardization. All rights reserved.

  18. A cancer specific cell-penetrating peptide, BR2, for the efficient delivery of an scFv into cancer cells.

    Directory of Open Access Journals (Sweden)

    Ki Jung Lim

    Full Text Available Cell-penetrating peptides (CPPs have proven very effective as intracellular delivery vehicles for various therapeutics. However, there are some concerns about non-specific penetration and cytotoxicity of CPPs for effective cancer treatments. Herein, based on the cell-penetrating motif of an anticancer peptide, buforin IIb, we designed several CPP derivatives with cancer cell specificity. Among the derivatives, a 17-amino acid peptide (BR2 was found to have cancer-specificity without toxicity to normal cells. After specifically targeting cancer cells through interaction with gangliosides, BR2 entered cells via lipid-mediated macropinocytosis. Moreover, BR2 showed higher membrane translocation efficiency than the well-known CPP Tat (49-57. The capability of BR2 as a cancer-specific drug carrier was demonstrated by fusion of BR2 to a single-chain variable fragment (scFv directed toward a mutated K-ras (G12V. BR2-fused scFv induced a higher degree of apoptosis than Tat-fused scFv in K-ras mutated HCT116 cells. These results suggest that the novel cell-penetrating peptide BR2 has great potential as a useful drug delivery carrier with cancer cell specificity.

  19. A cancer specific cell-penetrating peptide, BR2, for the efficient delivery of an scFv into cancer cells.

    Science.gov (United States)

    Lim, Ki Jung; Sung, Bong Hyun; Shin, Ju Ri; Lee, Young Woong; Kim, Da Jung; Yang, Kyung Seok; Kim, Sun Chang

    2013-01-01

    Cell-penetrating peptides (CPPs) have proven very effective as intracellular delivery vehicles for various therapeutics. However, there are some concerns about non-specific penetration and cytotoxicity of CPPs for effective cancer treatments. Herein, based on the cell-penetrating motif of an anticancer peptide, buforin IIb, we designed several CPP derivatives with cancer cell specificity. Among the derivatives, a 17-amino acid peptide (BR2) was found to have cancer-specificity without toxicity to normal cells. After specifically targeting cancer cells through interaction with gangliosides, BR2 entered cells via lipid-mediated macropinocytosis. Moreover, BR2 showed higher membrane translocation efficiency than the well-known CPP Tat (49-57). The capability of BR2 as a cancer-specific drug carrier was demonstrated by fusion of BR2 to a single-chain variable fragment (scFv) directed toward a mutated K-ras (G12V). BR2-fused scFv induced a higher degree of apoptosis than Tat-fused scFv in K-ras mutated HCT116 cells. These results suggest that the novel cell-penetrating peptide BR2 has great potential as a useful drug delivery carrier with cancer cell specificity.

  20. Identification and characterisation of the proteins bound by specific phage-displayed recombinant antibodies (scFv) obtained against Brazil nut and almond extracts.

    Science.gov (United States)

    de la Cruz, Silvia; Madrid, Raquel; García-García, Aina; Alcocer, Marcos; Martín, Rosario; González, Isabel; García, Teresa

    2018-03-01

    Almonds and Brazil nuts are widely consumed allergenic nuts whose presence must be declared according to food labelling regulations. Their detection in food products has been recently achieved by ELISA methods with recombinant antibodies (scFv) isolated against complete Brazil nut and almond protein extracts. The screening of phage-scFv libraries against complete protein extracts confers a series of advantages over the use of purified proteins, as recombinant proteins might alter their native folding. However, using this strategy, the nature of the target detected by phage-displayed antibodies remains unknown, and requires further research to identify whether they are nut allergens or other molecules present in the extract, but not related to their allergenic potential. Electrophoretic, chromatographic, immunological and spectrometric techniques revealed that the Brazil nut (BE95) and almond (PD1F6 and PD2C9) specific phage-scFvs detected conformational epitopes of the Brazil nut and almond 11S globulins, recognised by WHO/IUIS as Ber e 2 and Pru du 6 major allergens. Circular dichroism data indicated that severe heat treatment would entail loss of epitope structure, disabling scFv for target detection. The presence of important Brazil nut and almond allergens (Ber e 2 and Pru du 6) in foodstuffs can be determined by using phage-display antibodies BE95, PD1F6 and PD2C9 as affinity probes in ELISA. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  1. Activation of erythropoietin receptors by Friend viral gp55 and by erythropoietin and down-modulation by the murine Fv-2r resistance gene

    International Nuclear Information System (INIS)

    Hoatlin, M.E.; Kozak, S.L.; Kabat, D.; Lilly, F.; Chakraborti, A.; Kozak, C.A.

    1990-01-01

    The leukemogenic membrane glycoprotein (gp55) encoded by Friend spleen focus-forming virus appears to bind to erythropoietin receptors (EpoR) to stimulate erythroblastosis. To directly compare the effects of gp55 with erythropoietin (Epo), the authors produced retrovirions that encode either gp55, Epo, or EpoR. After infection with EpoR virus, interleukin 3-dependent DA-3 cells bound 125 I-labeled Epo and grew without interleukin 3 in the presence of Epo. These latter cells, but not parental DA-3 cells, became factor-independent after superinfection either with Epo virus or with Friend spleen focus-forming virus. In addition, Epo virus caused a disease in mice that mimicked Friend erythroleukemia. Although Fv-2 r homozygotes are susceptible to all other retroviral diseases, they are resistant to both Epo viral and Friend viral erythroleukemia. These results indicate that both gp55 and Epo stimulate EpoR and that the Fv-2 gene encodes a protein that controls response to these ligands. However, the Fv-2 protein is not EpoR because the corresponding genes map to opposite ends of mouse chromosome 9. These results have important implications for understanding signal transduction by EpoR and the role of host genetic variation in controlling susceptibility to an oncogenic protein

  2. Aptamers, antibody scFv, and antibody Fab' fragments: An overview and comparison of three of the most versatile biosensor biorecognition elements.

    Science.gov (United States)

    Crivianu-Gaita, Victor; Thompson, Michael

    2016-11-15

    The choice of biosensing elements is crucial for the development of the optimal biosensor. Three of the most versatile biosensing elements are antibody single-chain Fv fragments (scFv), antibody fragment-antigen binding (Fab') units, and aptamers. This article provides an overview of these three biorecognition elements with respects to their synthesis/engineering, various immobilization techniques, and examples of their use in biosensors. Furthermore, the final section of the review compares and contrasts their characteristics (time/cost of development, ease and variability of immobilization, affinity, stability) illustrating their advantages and disadvantages. Overall, scFv fragments are found to display the highest customizability (i.e. addition of functional groups, immobilizing peptides, etc.) due to recombinant synthesis techniques. If time and cost are an issue in the development of the biosensor, Fab' fragments should be chosen as they are relatively cheap and can be developed quickly from whole antibodies (several days). However, if there are sufficient funds and time is not a factor, aptamers should be utilized as they display the greatest affinity towards their target analytes and are extremely stable (excellent biosensor regenerability). Copyright © 2016 Elsevier B.V. All rights reserved.

  3. Neurocitoma no sistema nervoso central

    Directory of Open Access Journals (Sweden)

    José Torquato Severo

    1973-03-01

    Full Text Available É relatado o caso de uma paciente com 6 anos de idade, hospitalizada com síndrome de hipertensão intracraniana. Após o exame neuro-radiológico que evidenciou processo expansivo frontal direito, a paciente foi submetida à cirurgia, durante a qual ocorreu o óbito. O exame histo-patológico do material retirado durante o ato operatório, permitiu o diagnóstico de neurocitoma, tumor raro no sistema nervoso central.

  4. Sistema prisional e direitos humanos

    OpenAIRE

    Mendes, André Pacheco Teixeira

    2015-01-01

    O volume 4 da Série Clínicas dos Cadernos FGV DIREITO RIO traz quatro trabalhos originais relacionados ao tema do Sistema Prisional e Direitos Humanos. A obra contempla pareceres jurídicos sobre violações aos direitos dos presos nas unidades prisionais do estado do Rio de Janeiro, tendo por objeto: (i) emprego ilegal de laxante em custodiados e visitantes suspeitos de carregarem drogas; (ii) falta de água própria para o consumo humano; (iii) restrição ao banho de sol; e (iv) condições inadequ...

  5. Profile data from CTD casts aboard the F/V Ocean Explorer in the Arctic Ocean and Beaufort Sea from 2008-08-06 to 2008-08-22 (NODC Accession 0001920)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This profile data aboard the F/V Ocean Explorer in the Arctic Ocean and Beaufort Sea from August 6, 2008 to August 22, 2008 was supported by the Minerals Management...

  6. Sistema de videovigilancia utilizando recursos de LAN

    OpenAIRE

    Fernández García, David

    2007-01-01

    El objetivo principal de este proyecto es realizar un sistema de información que sea capaz de efectuar video vigilancia. Este sistema de información está formado por un servidor y un número, en principio, limitado de clientes

  7. A compact phage display human scFv library for selection of antibodies to a wide variety of antigens

    Directory of Open Access Journals (Sweden)

    Kristensen Peter

    2009-01-01

    Full Text Available Abstract Background Phage display technology is a powerful new tool for making antibodies outside the immune system, thus avoiding the use of experimental animals. In the early days, it was postulated that this technique would eventually replace hybridoma technology and animal immunisations. However, since this technology emerged more than 20 years ago, there have only been a handful reports on the construction and application of phage display antibody libraries world-wide. Results Here we report the simplest and highly efficient method for the construction of a highly useful human single chain variable fragment (scFv library. The least number of oligonucleotide primers, electroporations and ligation reactions were used to generate a library of 1.5 × 108 individual clones, without generation of sub-libraries. All possible combinations of heavy and light chains, among all immunoglobulin isotypes, were included by using a mixture of primers and overlapping extension PCR. The key difference from other similar libraries was the highest diversity of variable gene repertoires, which was derived from 140 non-immunized human donors. A wide variety of antigens were successfully used to affinity select specific binders. These included pure recombinant proteins, a hapten and complex antigens such as viral coat proteins, crude snake venom and cancer cell surface antigens. In particular, we were able to use standard bio-panning method to isolate antibody that can bind to soluble Aflatoxin B1, when using BSA-conjugated toxin as a target, as demonstrated by inhibition ELISA. Conclusion These results suggested that by using an optimized protocol and very high repertoire diversity, a compact and efficient phage antibody library can be generated. This advanced method could be adopted by any molecular biology laboratory to generate both naïve or immunized libraries for particular targets as well as for high-throughput applications.

  8. A human scFv antibody that targets and neutralizes high molecular weight pathogenic amyloid-β oligomers.

    Science.gov (United States)

    Sebollela, Adriano; Cline, Erika N; Popova, Izolda; Luo, Kevin; Sun, Xiaoxia; Ahn, Jay; Barcelos, Milena A; Bezerra, Vanessa N; Lyra E Silva, Natalia M; Patel, Jason; Pinheiro, Nathalia R; Qin, Lei A; Kamel, Josette M; Weng, Anthea; DiNunno, Nadia; Bebenek, Adrian M; Velasco, Pauline T; Viola, Kirsten L; Lacor, Pascale N; Ferreira, Sergio T; Klein, William L

    2017-07-03

    Brain accumulation of soluble oligomers of the amyloid-β peptide (AβOs) is increasingly considered a key early event in the pathogenesis of Alzheimer's disease (AD). A variety of AβO species have been identified, both in vitro and in vivo, ranging from dimers to 24mers and higher order oligomers. However, there is no consensus in the literature regarding which AβO species are most germane to AD pathogenesis. Antibodies capable of specifically recognizing defined subpopulations of AβOs would be a valuable asset in the identification, isolation, and characterization of AD-relevant AβO species. Here, we report the characterization of a human single chain antibody fragment (scFv) denoted NUsc1, one of a number of scFvs we have identified that stringently distinguish AβOs from both monomeric and fibrillar Aβ. NUsc1 readily detected AβOs previously bound to dendrites in cultured hippocampal neurons. In addition, NUsc1 blocked AβO binding and reduced AβO-induced neuronal oxidative stress and tau hyperphosphorylation in cultured neurons. NUsc1 further distinguished brain extracts from AD-transgenic mice from wild type (WT) mice, and detected endogenous AβOs in fixed AD brain tissue and AD brain extracts. Biochemical analyses indicated that NUsc1 targets a subpopulation of AβOs with apparent molecular mass greater than 50 kDa. Results indicate that NUsc1 targets a particular AβO species relevant to AD pathogenesis, and suggest that NUsc1 may constitute an effective tool for AD diagnostics and therapeutics. © 2017 International Society for Neurochemistry.

  9. Anti-HER2 antibody and ScFvEGFR-conjugated antifouling magnetic iron oxide nanoparticles for targeting and magnetic resonance imaging of breast cancer

    Directory of Open Access Journals (Sweden)

    Chen H

    2013-10-01

    Full Text Available Hongwei Chen,1,* Liya Wang,1,2,* Qiqi Yu,1,2 Weiping Qian,3 Diana Tiwari,1 Hong Yi,4 Andrew Y Wang,5 Jing Huang,1,2 Lily Yang,3 Hui Mao1,2 1Department of Radiology and Imaging Sciences, 2Center for Systems Imaging, 3Department of Surgery, Emory University School of Medicine, 4Robert Apkarian Electron Microscopy Core, Emory University, Atlanta, GA, 5Ocean NanoTech LLC, Springdale, AK, USA *These authors contributed equally to this work Abstract: Antifouling magnetic iron oxide nanoparticles (IONPs coated with block copolymer poly(ethylene oxide-block-poly(γ-methacryloxypropyltrimethoxysilane (PEO-b-PγMPS were investigated for improving cell targeting by reducing nonspecific uptake. Conjugation of a HER2 antibody, Herceptin®, or a single chain fragment (ScFv of antibody against epidermal growth factor receptor (ScFvEGFR to PEO-b-PγMPS-coated IONPs resulted in HER2-targeted or EGFR-targeted IONPs (anti-HER2-IONPs or ScFvEGFR-IONPs. The anti-HER2-IONPs bound specifically to SK-BR-3, a HER2-overexpressing breast cancer cell line, but not to MDA-MB-231, a HER2-underexpressing cell line. On the other hand, the ScFvEGFR-IONPs showed strong reactivity with MDA-MB-231, an EGFR-positive human breast cancer cell line, but not with MDA-MB-453, an EGFR-negative human breast cancer cell line. Transmission electron microscopy revealed internalization of the receptor-targeted nanoparticles by the targeted cancer cells. In addition, both antibody-conjugated and non-antibody-conjugated IONPs showed reduced nonspecific uptake by RAW264.7 mouse macrophages in vitro. The developed IONPs showed a long blood circulation time (serum half-life 11.6 hours in mice and low accumulation in both the liver and spleen. At 24 hours after systemic administration of ScFvEGFR-IONPs into mice bearing EGFR-positive breast cancer 4T1 mouse mammary tumors, magnetic resonance imaging revealed signal reduction in the tumor as a result of the accumulation of the targeted IONPs

  10. IG and TR single chain fragment variable (scFv) sequence analysis: a new advanced functionality of IMGT/V-QUEST and IMGT/HighV-QUEST.

    Science.gov (United States)

    Giudicelli, Véronique; Duroux, Patrice; Kossida, Sofia; Lefranc, Marie-Paule

    2017-06-26

    IMGT®, the international ImMunoGeneTics information system® ( http://www.imgt.org ), was created in 1989 in Montpellier, France (CNRS and Montpellier University) to manage the huge and complex diversity of the antigen receptors, and is at the origin of immunoinformatics, a science at the interface between immunogenetics and bioinformatics. Immunoglobulins (IG) or antibodies and T cell receptors (TR) are managed and described in the IMGT® databases and tools at the level of receptor, chain and domain. The analysis of the IG and TR variable (V) domain rearranged nucleotide sequences is performed by IMGT/V-QUEST (online since 1997, 50 sequences per batch) and, for next generation sequencing (NGS), by IMGT/HighV-QUEST, the high throughput version of IMGT/V-QUEST (portal begun in 2010, 500,000 sequences per batch). In vitro combinatorial libraries of engineered antibody single chain Fragment variable (scFv) which mimic the in vivo natural diversity of the immune adaptive responses are extensively screened for the discovery of novel antigen binding specificities. However the analysis of NGS full length scFv (~850 bp) represents a challenge as they contain two V domains connected by a linker and there is no tool for the analysis of two V domains in a single chain. The functionality "Analyis of single chain Fragment variable (scFv)" has been implemented in IMGT/V-QUEST and, for NGS, in IMGT/HighV-QUEST for the analysis of the two V domains of IG and TR scFv. It proceeds in five steps: search for a first closest V-REGION, full characterization of the first V-(D)-J-REGION, then search for a second V-REGION and full characterization of the second V-(D)-J-REGION, and finally linker delimitation. For each sequence or NGS read, positions of the 5'V-DOMAIN, linker and 3'V-DOMAIN in the scFv are provided in the 'V-orientated' sense. Each V-DOMAIN is fully characterized (gene identification, sequence description, junction analysis, characterization of mutations and amino

  11. El sistema reticulo-endotelial

    Directory of Open Access Journals (Sweden)

    Carlos M. Pava

    1936-05-01

    Full Text Available El lector aficionado a los estudios histológicos que hace unos veinte años hubiese hojeado algún tratado nuevo sobre la materia, habría encontrado el término de "Sistema retículo-endotelial", sin duda extraño y desconocido para él, porque solamente entonces empezó a figurar no sólo como tal, sino principalmente como la expresión de una nueva agrupación de tejidos cuya morfología era semejante en líneas generales, y cuyo funcionamiento, a pesar de estar diseminado por todo el organismo, es de una precisión e igualdad admirables.

  12. Immanuel Wallerstein e o moderno sistema mundial

    Directory of Open Access Journals (Sweden)

    José Jobson de Andrade Arruda

    1983-12-01

    Full Text Available (primeiro parágrafo do texto Immanuel Wallerstein é diretor do Centro Fernand Braudel para o Estudo de Economias, Sistemas Históricos e Civilizacionais (Binghamton, N.Y. e editor da revista trimestral Review. Seu projeto mais amplo é a busca dos elementos determinantes do sistema mundial moderno. Para tanto, dividiu o seu trabalho em quatro partes principais, às quais con-sidera as quatro épocas fundamentais apresentadas pelo dito sistema até hoje. O primeiro volume, objeto desta resenha crítica, cobre o período de 1450 a 1640, correspondendo ao estudo das origens e condições primitivas do sistema mundial até então exclusivamente um sistema europeu. O segundo volume, já publicado, ocupar-se-ia da consolidação do sis-tema entre 1640 e 1815. O terceiro volume, referente ao período de 1815 a 1917, estará centrado na recriação do sistema decorrente das vertiginosas transformações resultantes do dinamismo tecnológico e do avanço do industrialismo no século XIX. O quarto volume cuidará da consolidação da economia — mundo capitalista, a partir de 1917 até a atualidade, e das tensões "revolucionárias" particulares que esta conso lidação tem provocado.

  13. El sistema deportivo. Amenazas y oportunidades

    Directory of Open Access Journals (Sweden)

    Javier Olivera Betrán

    2006-06-01

    Full Text Available Aplicando la teoría de sistemas de Ludwig von Bertalanffy, el deporte es un sistema. En un sistema se da un conjunto de unidades interrelacionadas de tal manera que el comportamiento de cada parte depende del estado de todas las otras, pues todas se encuentran en una estructura que las interconecta. En el enfoque sistémico, el concepto tradicional de energía es reemplazado por el de información, y el de causa-efecto por el de estructura y realimentación. El deporte es una realidad social total, por lo que participa de manera implícita y/o explícita en los procesos humanos más diversos a través de los distintos procedimientos que corresponden a nuestra organización sociocultural. El deporte se configura en estructuras de alta complejidad, las cuales están constituidas por sistemas de sistemas cuya comprensión y estudio representan un formidable reto. El estudio del deporte como sistema debe efectuarse desde la lógica estructural y sistémica a través de la cual nos permita indagar con éxito su configuración esencial, sus elementos fundamentales y el proceso de interactuación con los subsistemas que pertenecen al sistema deporte y con los otros del sistema sociocultural dominante. El deporte es un sistema dinámico que interactúa continuamente mediante un doble proceso, intrínseco a través de una constante metamorfosis entre los distintos subsistemas que lo integran y extrínseco por medio de un ajuste permanente con el contexto social, cultural y medioambiental.

  14. Pharmacological efficacy of anti-IL-1β scFv, Fab and full-length antibodies in treatment of rheumatoid arthritis.

    Science.gov (United States)

    Qi, Jianying; Ye, Xianlong; Ren, Guiping; Kan, Fangming; Zhang, Yu; Guo, Mo; Zhang, Zhiyi; Li, Deshan

    2014-02-01

    Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease that mainly causes the synovial joint inflammation and cartilage destruction. Interleukin-1β (IL-1β) is an important proinflammatory cytokine involved in the pathogenesis of RA. In this study, we constructed and expressed anti-IL-1β-full-length antibody in CHO-K1-SV, anti-IL-1β-Fab and anti-IL-1β-scFv in Rosetta. We compared the therapeutic efficacy of three anti-IL-1β antibodies for CIA mice. Mice with CIA were subcutaneously injected with humanized anti-IL-1β-scFv, anti-IL-1β-Fab or anti-IL-1β-full-length antibody. The effects of treatment were determined by arthritis severity score, autoreactive humoral, cellular immune responses, histological lesion and cytokines production. Compared with anti-IL-1β-scFv treatments, anti-IL-1β-Fab and anti-IL-1β-full-length antibody therapy resulted in more significant effect in alleviating the severity of arthritis by preventing bone damage and cartilage destruction, reducing humoral and cellular immune responses, and down-regulating the expression of IL-1β, IL-6, IL-2, IFN-γ, TNF-α and MMP-3 in inflammatory tissue. The therapeutic effects of anti-IL-1β-Fab and anti-IL-1β-full-length antibodies on CIA mice had no significant difference. However, production of anti-IL-1β-full-length antibody in eukaryotic system is, in general, time-consuming and more expensive than that of anti-IL-1β-Fab in prokaryotic systems. In conclusion, as a small molecule antibody, anti-IL-1β-Fab is an ideal candidate for RA therapy. Copyright © 2013 Elsevier Ltd. All rights reserved.

  15. BASALIT trial: double-blind placebo-controlled allergen immunotherapy with rBet v 1-FV in birch-related soya allergy.

    Science.gov (United States)

    Treudler, R; Franke, A; Schmiedeknecht, A; Ballmer-Weber, B; Worm, M; Werfel, T; Jappe, U; Biedermann, T; Schmitt, J; Brehler, R; Kleinheinz, A; Kleine-Tebbe, J; Brüning, H; Ruëff, F; Ring, J; Saloga, J; Schäkel, K; Holzhauser, T; Vieths, S; Simon, J C

    2017-08-01

    Conflicting results exist on the effect of allergen immunotherapy (AIT) on pollen-related food allergy. We aimed to investigate the efficacy of one-year AIT with the folding variant (FV) of recombinant (r) Bet v 1 on birch-related soya allergy. Of 138 subjects with Bet v 1 sensitization, 82 were positive at double-blind placebo-controlled food challenge (DBPCFC) with soya. A total of 56 of 82 were randomized in the ratio of 2:1 (active: placebo). Per-protocol population (PPP) had received ≥150 μg of allergen or placebo preparation. lowest observed adverse effect levels (LOAEL), postinterventional occurrence of objective signs (objS) at any dose level, sIgE/IgG4 against Bet v 1 and Gly m 4. Between-group changes were investigated (ancova, Mann-Whitney U-test, Fisher exact test). Baseline characteristics including LOAELs were comparable in both groups with objS and subjS occurring in 82% and 95% of active (n = 38) vs 78% and 83% of placebo group (n = 18). After AIT, objS occurred in 24% and 47%, respectively. LOAEL group differences showed a beneficial tendency (P = 0.081) for LOAEL objective in PPP (30 active, 15 placebo). sIgG4 raised only in active group (Bet v 1: P = 0.054, Gly m 4: P = 0.037), and no relevant changes occurred for sIgE. Only 56% of the intended sample size was recruited. For the first time, we present data on the effect of rBet v 1-FV on birch-related soya allergy. rBet v 1-FV AIT induced significant immunogenic effects. Clinical assessment showed a tendency in favour of the active group but did not reach statistical significance. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  16. Downregulation of Extracellular Matrix Metalloproteinase Inducer by scFv-M6-1B9 Intrabody Suppresses Cervical Cancer Invasion Through Inhibition of Urokinase-Type Plasminogen Activator.

    Science.gov (United States)

    Panich, Tipattaraporn; Tragoolpua, Khajornsak; Pata, Supansa; Tayapiwatana, Chatchai; Intasai, Nutjeera

    2017-02-01

    Overexpression of extracellular matrix metalloproteinase inducer (EMMPRIN) accelerates tumor invasion and metastasis via activation of matrix metalloproteinases (MMPs) and urokinase-type plasminogen activator (uPA) expression. The authors were interested in whether the scFv-M6-1B9 intrabody against EMMPRIN that retains EMMPRIN in endoplasmic reticulum could be a potential tool to suppress cervical cancer invasion through inhibition of uPA. The chimeric adenoviral vector Ad5/F35-scFv-M6-1B9 was transferred into human cervical carcinoma HeLa cells to produce the scFv-M6-1B9 intrabody against EMMPRIN. Cell surface expression of EMMPRIN, the membrane-bound uPA, the enzymatic activity of secreted uPA, and the invasion ability were analyzed. The scFv-M6-1B9 intrabody successfully diminished the cell surface expression of EMMPRIN and the membrane-bound uPA on HeLa cells. uPA activity from tissue culture media of EMMPRIN-downregulated HeLa cells was decreased. The invasion ability of HeLa cells harboring scFv-M6-1B9 intrabody was also suppressed. These results suggested that the scFv-M6-1B9 intrabody might represent a potential approach for invasive cervical cancer treatment. The application of scFv-M6-1B9 intrabody in animal experiments and preclinical studies would be investigated further.

  17. CONTROL PREDICTIVO HIBRIDO PARA FLOTACION DE MINERALES

    OpenAIRE

    PUTZ DE LA FUENTE, EDUARDO IGNACIO

    2014-01-01

    La minería es el sector de mayor actividad en la economía nacional, posicionando a Chile como el mayor productor y exportador de cobre a nivel mundial. Sin embargo, aún existen grandes desafíos que buscan mitigar el aumento en los costos de la energía y la disminución de las leyes de mineral, así como la promoción de nuevas tecnologías. Bajo este contexto, las tecnologías de automatización se presentan como una alternativa viable y necesaria para aumentar la productividad y confiabil...

  18. Cogeneration Systems; Sistemas de Cogeneracion

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez M, Manuel F; Huante P, Liborio; Romo M, Cesar A [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)

    2006-07-01

    The present article deals on relevant aspects on the subject of cogeneration within the Mexican territorial limits. In the first place it is presented the role of Mexico in terms of its cogeneration potential, the type of service that has obtained from this predominant modality of cogeneration for self-supplying, the most propitious sectors to develop it, its legislations on the matter, the projects made for the implementation of cogeneration plants, as well as the existing cogeneration schemes for its respective optimization proposals. Without leaving out the analysis on the different types of evaluation on the efficiency of cogeneration systems and the aspects to consider for the election of a generation cycle. [Spanish] El presente articulo trata sobre aspectos relevantes en materia de cogeneracion dentro de los limites territoriales de la nacion mexicana. Se muestra en primer lugar el papel de Mexico en terminos de su potencial de cogeneracion, el tipo de servicio que ha obtenido de esta predominantemente (modalidad de cogeneracion para autoabastecimiento), los sectores mas propicios para desarrollarla, sus legislaciones al respecto, los proyectos realizados para la implementacion de plantas de cogeneracion, asi como los esquemas de cogeneracion existentes con sus respectivas propuestas de optimizacion. Sin dejar de lado el analisis sobre los distintos tipos de evaluacion de la eficiencia de sistemas de cogeneracion y los aspectos a considerar para la eleccion de un ciclo de generacion.

  19. Regulación neuroendocrina del sistema inmune

    OpenAIRE

    Caballero Hernández, Diana; Tamez Guerra, Reyes S.; Rodríguez Padilla, Cristina; Tamez Guerra, Patricia; Weber, Richard J.; Gómez Flores, Ricardo

    2001-01-01

    El sistema inmune recibe señales del sistema nervioso central (cerebro) vía el sistema nervioso autónomo y el sistema endocrino. El sistema inmune, a su vez, envía información al cerebro vía citocinas. Este sistema de retroalimentación es vital para el funcionamiento adecuado del organismo en situaciones normales, y en aquellas en las que la homeostasis se ve perturbada, como en casos de estrés, consumo de drogas (terapéuticas o de abuso), enfermedades infecciosas y...

  20. Sistemas de gestión ambiental normalizados

    OpenAIRE

    Fronti de García, Luisa; Pahlen, Ricardo José María; D’Onofrio, Paula Alejandra

    2004-01-01

    La contabilidad ambiental presupone la existencia de un sistema de gestión ambiental. Dentro del sistema integral de la organización, el sistema de gestión ambiental permite la obtención de información oportuna y segura. Podríamos definir el sistema de gestión ambiental de un ente como el conjunto de medidas destinadas a prevenir el impacto negativo de las actividades del ente sobre el medio ambiente. El sistema de gestión ambiental (SGA) es aquella parte del sistema de gestión total que incl...

  1. Contexto General de los Sistemas Agrarios

    Directory of Open Access Journals (Sweden)

    Vélez Escobar Norberto

    1989-12-01

    Full Text Available Esta ponencia introdujo y delimitó el campo de trabajo para el panel: Formas o Sistemas de Producción Agraria. En ella se presentó y discutió la definición del concepto de FORMA DE PRODUCCION e igualmente planteó la taxonomía más general o "agrupamientos" de los sistemas de producción agraria en Colombia. Se identificaron cinco grupos de formas, en proceso de estructuración, empresariales, campesinas, de economía natural y experimentales; y a continuación se caracterizó y explicó cada uno de los cinco agrupamientos. La ponencia terminó sustentando la validez del trabajo académico basado en el enfoque de sistemas y presentó la evolución reciente de esta forma de trabajo en la Universidad Nacional, Seccional Medellín.

  2. SCODA para el Desarrollo de Sistemas Multiagente

    Directory of Open Access Journals (Sweden)

    Jesús A. Román

    2011-12-01

    Full Text Available El planteamiento de implementar sistemas multiagente de forma que puedan reutilizarse con distintos objetivos, dota a los desarrolladores de una metodología más eficaz en sus fines. Este artículo presenta SCODA, una arquitectura basada en cinco principios: estandaridad, especialización, facilidad de implementación, reutilización y computación distribuida. SCODA pretende facilitar el desarrollo de sistemas multiagente, basándose en el concepto de pequeños grupos de agentes denominados Comunidades Inteligentes Especializadas (CIE. Las CIE cuentan con funcionalidades específicas que permiten llevar a cabo la implementación de sistemas multiagente de forma escalable, dentro del marco de SCODA, pudiendo ser reutilizadas en diversos desarrollos.

  3. Comparison of a mouse and a novel human scFv-SNAP-auristatin F drug conjugate with potent activity against EGFR-overexpressing human solid tumor cells

    Directory of Open Access Journals (Sweden)

    Woitok M

    2017-07-01

    Full Text Available Mira Woitok,1,2 Diana Klose,1 Stefano Di Fiore,1 Wolfgang Richter,3 Christoph Stein,1 Gerrit Gresch,1 Elena Grieger,1 Stefan Barth,1 Rainer Fischer,1,2 Katharina Kolberg,1,* Judith Niesen1,* 1Fraunhofer Institute for Molecular Biology and Applied Ecology (IME, Aachen, Germany; 2Institute of Molecular Biotechnology (Biology VII, RWTH Aachen University, Aachen, Germany; 3Tube Pharmaceuticals GmbH, Vienna, Austria *These authors contributed equally to this work Abstract: Antibody–drug conjugates (ADCs can deliver toxins to specific targets such as tumor cells. They have shown promise in preclinical/clinical development but feature stoichiometrically undefined chemical linkages, and those based on full-size antibodies achieve only limited tumor penetration. SNAP-tag technology can overcome these challenges by conjugating benzylguanine-modified toxins to single-chain fragment variables (scFvs with 1:1 stoichio­metry while preserving antigen binding. Two (human and mouse scFv-SNAP fusion proteins recognizing the epidermal growth factor receptor (EGFR were expressed in HEK 293T cells. The purified fusion proteins were conjugated to auristatin F (AURIF. Binding activity was confirmed by flow cytometry/immunohistochemistry, and cytotoxic activity was confirmed by cell viability/apoptosis and cell cycle arrest assays, and a novel microtubule dynamics disassembly assay was performed. Both ADCs bound specifically to their target cells in vitro and ex vivo, indicating that the binding activity of the scFv-SNAP fusions was unaffected by conjugation to AURIF. Cytotoxic assays confirmed that the ADCs induced apoptosis and cell cycle arrest at nanomolar concentrations and microtubule disassembly. The SNAP-tag technology provides a platform for the development of novel ADCs with defined conjugation sites and stoichiometry. We achieved the stable and efficient linkage of AURIF to human or murine scFvs using the SNAP-tag technology, offering a strategy to

  4. Targeting the active site of the placental isozyme of alkaline phosphatase by phage-displayed scFv antibodies selected by a specific uncompetitive inhibitor

    Directory of Open Access Journals (Sweden)

    Kala Mrinalini

    2005-12-01

    Full Text Available Abstract Background The isozymes of alkaline phosphatase, the tissue non-specific, intestinal and placental, have similar properties and a high degree of identity. The placental isozyme (PLAP is an oncofetal antigen expressed in several malignancies including choriocarcinoma, seminoma and ovarian carcinoma. We had earlier attempted to isolate PLAP-specific scFv from a synthetic human immunoglobulin library but were unable to do so, presumably because of the similarity between the isozymes. In this work, we have employed a PLAP-specific uncompetitive inhibitor, L-Phe-Gly-Gly, to select isozyme specific scFvs. An uncompetitive inhibitor binds to the enzyme in the presence of substrate and stabilizes the enzyme-substrate complex. Several uncompetitive inhibitors have varying degrees of isozyme specificity for human alkaline phosphatase isozymes. A specific uncompetitive inhibitor would be able to unmask conformational differences between the otherwise very similar molecules. Also, such inhibitors would be directed to regions at/close to the active site of the enzyme. In this work, the library was first incubated with PLAP and the bound clones then eluted by incubation with L-Phe-Gly-Gly along with the substrate, para-nitro phenyl phosphate (pNPP. The scFvs were then studied with regard to the biochemical modulation of their binding, isozyme specificity and effect on enzyme activity. Results Of 13 clones studied initially, the binding of 9 was inhibited by L-Phe-Gly-Gly (with pNPP and 2 clones were inhibited by pNPP alone. Two clones had absolute and 2 clones had partial specificity to PLAP. Two clones were cross-reactive with only one other isozyme. Three scFv clones, having an accessible His6-tag, were purified and studied for their modulation of enzyme activity. All the three scFvs inhibited PLAP activity with the kinetics of competitive inhibition. Cell ELISA could demonstrate binding of the specific scFvs to the cell surface expressed PLAP

  5. Psiconeuroinmunología: Conexiones entre sistema nervioso y sistema inmune

    Directory of Open Access Journals (Sweden)

    García López, Luis Joaquín

    2008-09-01

    Full Text Available La psiconeuroinmunología es una nueva parcela de la ciencia que apenas lleva tres décadas, pero que en los últimos años ha sido objeto de una enorme atención por diversos grupos de investigación. El articulo pretende exponer brevemente cómo están organizados los sistemas inmune y neuroendocrino, para después presentar los hallazgos que hasta el momento intentan dar explicación de las relaciones entre ambos con el sistema nervioso, así como las influencias de unos sobre otros, más concretamente expresar la relación entre los tres sistemas responsables de la defensa del organismo y, más ampliamente, de su mantenimiento homeostático. Cuando se trata de amenazas, el sistema nervioso central tiene que decidir qué sistema se pone en marcha, el sistema de estrés como energía para el ataque o la huida (un animal que nos quiere atacar, el sistema inmune para neutralizar el antígeno (un virus que nos quiere atacar o ambos (la energía es necesaria también para el sistema inmune. Como parece ser que en muchas ocasiones la depresión aparece tras los esfuerzos infructuosos de puesta en marcha de estrategias de afrontamiento, también hemos repasado sucintamente algunas investigaciones que relacionan al sistema inmune con la depresión, así como con los trastornos del sueño. Se finaliza con las aportaciones que la psicología puede ofrecer a nivel terapéutico.

  6. Estudio de los Sistemas Inteligentes de Transporte

    OpenAIRE

    Javato Martín, Moisés

    2015-01-01

    En este trabajo se estudia el estado actual de los Sistemas Inteligentes de Transporte (ITS) en Estados Unidos y Europa, a través de ejemplos reales. En primer lugar, se da una visión general del tema, definiendo los aspectos y actores clave y explicando las áreas de la gestión del tráfico y del vehículo conectado. A continuación, se aborda el desarrollo de los sistemas ITS en el ámbito de la Unión Europea, explicando las principales instituciones y programas de financiación europeos, así com...

  7. Categorizando a los usuarios de sistemas digitales

    OpenAIRE

    Hernández y Hernández, Denise; Ramírez Martinell, Alberto; Cassany, Daniel

    2014-01-01

    Repasamos las categorías más utilizadas para aludir a las características de las personas que utilizan algún tipo de sistema digital, ya sea por su grado de participación en la comunicación global y en los sistemas web -1.0 o 2.0-; por la actitud que toman ante el contenido digital -consumidores o productores-; por su intermitencia en el mundo digital -residentes o visitantes-; o por el momento histórico en el que nacieron -nativos digitales o inmigrantes digitales-, con el propósito de refle...

  8. Sistema de realidad aumentada para aplicaciones Android

    OpenAIRE

    Fernández Sánchez, Natalia Mercedes

    2012-01-01

    El objetivo principal de este proyecto es la creación de un sistema de realidad aumentada para el sistema operativo Android con idea de que sea utilizado para la creación de un videojuego, pero que a su vez sea lo suficientemente versátil como para poder utilizarse en otro tipo de aplicaciones. De este objetivo principal podemos obtener los objetivos secundarios: - Conocer las características que ofrecen los teléfonos móviles y como éstas pueden ser aprovechadas para la crea...

  9. CATEGORIZANDO A LOS USUARIOS DE SISTEMAS DIGITALES.

    OpenAIRE

    Denise Hernández y Hernández; Alberto Ramírez-Martinell; Daniel Cassany

    2014-01-01

    Repasamos las categorías más utilizadas para aludir a las características de las personas que utilizan algún tipo de sistema digital, ya sea por su grado de participación en la comu- nicación global y en los sistemas web -1.0 o 2.0-; por la actitud que toman ante el contenido digital -consumidores o productores-; por su intermitencia en el mundo digital -residentes o visitantes-; o por el momento histórico en el que nacieron -nativos digitales o inmigrantes digitales-, con el propósito de ref...

  10. El sistema de los status de Eugrafio

    OpenAIRE

    Pirovano, Luigi

    2009-01-01

    [ES] Recogiendo y analizando las glosas retóricas del Commentum Terenti atribuido a Eugrafio, es posible reconstruir con un nivel de precisión bastante elevado el sistema de los status utilizado por el comentarista, muy parecido -aunque no igual- a los sistemas «de trece status» que podemos encontrar en los Rhetores Latini Minores. Este análisis nos permite apreciar la naturaleza unitaria del comentario y precisar mejor cuál fue la personalidad y la auténtica finalidad de Eugrafio, a un tiemp...

  11. Sistemas de custos para pequenas empresas industriais

    OpenAIRE

    Moraes, João Vicente de

    2002-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia de Produção. Este trabalho define um sistema de custos para pequenas empresas industriais, o qual permite identificar o custo, resultado e margem de contribuição por produto e ponto de equilíbrio , considerando o período de apuração do resultado. O sistema proposto é aplicado em um caso prático, em uma empresa estabelecida na cidade de Cascavel-PR., que produz rodas ...

  12. SCODA para el Desarrollo de Sistemas Multiagente

    OpenAIRE

    Román Gallego, Jesús Ángel; Tapia Martínez, Dante I.; Corchado Rodríguez, Juan M.

    2017-01-01

    El planteamiento de implementar sistemas multiagente de forma que puedan reutilizarse con distintos objetivos, dota a los desarrolladores de una metodología más eficaz en sus fines. Este artículo presenta SCODA, una arquitectura basada en cinco principios: estandaridad, especialización, facilidad de implementación, reutilización y computación distribuida. SCODA pretende facilitar el desarrollo de sistemas multiagente, basándose en el concepto de pequeños grupos de agentes denominados Comunida...

  13. Desarrollo de un sistema de reconocimiento facial

    OpenAIRE

    Vivas Imparato, Abdón Alejandro

    2014-01-01

    El objetivo principal alrededor del cual se desenvuelve este proyecto es el desarrollo de un sistema de reconocimiento facial. Entre sus objetivos específicos se encuentran: realizar una primera aproximación sobre las técnicas de reconocimiento facial existentes en la actualidad, elegir una aplicación donde pueda ser útil el reconocimiento facial, diseñar y desarrollar un programa en MATLAB que lleve a cabo la función de reconocimiento facial, y evaluar el funcionamiento del sistema desarroll...

  14. Sistema de gestión documental

    OpenAIRE

    Fernández Gómez, Javier

    2015-01-01

    Esta memoria expone la planificación, el análisis, el diseño y el desarrollo de un sistema de gestión documental, en adelante SGD, configurable, es decir, adaptable a un ámbito determinado. El SGD consta de dos grupos de interfaces o funcionalidades claramente diferenciadas: administración y gestión documental. Aquesta memòria exposa la planificació, l'anàlisi, el disseny i el desenvolupament d'un sistema de gestió documental, d'ara endavant SGA, configurable, és a dir, adaptable a un àmbi...

  15. Implementação de um sistema SIP para o sistema operacional Linux

    OpenAIRE

    Davison Gonzaga da Silva

    2003-01-01

    Resumo: Este trabalho apresenta a implementação de um Sistema de VoIP usando o Protocolo SIP. Este Sistema SIP foi desenvolvido para o Linux, usando-se a linguagem C++ em conjunto com a biblioteca QT. O Sistema SIP é composto de três entidades básicas: o Terminal SIP, o Proxy e o Servidor de Registros. O Terminal SIP é a entidade responsável por estabelecer sessões SIP com outros Terminais SIP. Para o Terminal SIP, foi desenvolvida uma biblioteca de acesso à placa de áudio, que permite a modi...

  16. Characterization of an engineered human purine nucleoside phosphorylase fused to an anti-her2/neu single chain Fv for use in ADEPT

    Directory of Open Access Journals (Sweden)

    Wu Anna M

    2009-12-01

    Full Text Available Abstract Background Antibody Directed Enzyme Prodrug Therapy (ADEPT can be used to generate cytotoxic agents at the tumor site. To date non-human enzymes have mainly been utilized in ADEPT. However, these non-human enzymes are immunogenic limiting the number of times that ADEPT can be administered. To overcome the problem of immunogenicity, a fully human enzyme, capable of converting a non-toxic prodrug to cytotoxic drug was developed and joined to a human tumor specific scFv yielding a fully human targeting agent. Methods A double mutant of human purine nucleoside phosphorylase (hDM was developed which unlike the human enzyme can cleave adenosine-based prodrugs. For tumor-specific targeting, hDM was fused to the human anti-HER2/neu single chain Fv (scFv, C6 MH3B1. Enzymatic activity of hDM with its natural substrates and prodrugs was determined using spectrophotomeric approaches. A cell proliferation assay was used to assess the cytotoxicity generated following conversion of prodrug to drug as a result of enzymatic activity of hDM. Affinity of the targeting scFv, C6 MH3B1 fused to hDM to Her2/neu was confirmed using affinity chromatography, surface plasmon resonance, and flow-cytometry. Results In vitro hDM-C6 MH3B1 binds specifically to HER2/neu expressing tumor cells and localizes hDM to tumor cells, where the enzymatic activity of hDM-C6 MH3B1, but not the wild type enzyme, results in phosphorolysis of the prodrug, 2-fluoro-2'-deoxyadenosine to the cytotoxic drug 2-fluoroadenine (F-Ade causing inhibition of tumor cell proliferation. Significantly, the toxic small drug diffuses through the cell membrane of HER2/neu expressing cells as well as cells that lack the expression of HER2/neu, causing a bystander effect. F-Ade is toxic to cells irrespective of their growth rate; therefore, both the slowly dividing tumor cells and the non-dividing neighboring stromal cells that support tumor growth should be killed. Analysis of potential novel MHCII

  17. Estabilización robusta de sistemas lineales

    Directory of Open Access Journals (Sweden)

    Horacio Leyva Castellanos

    2013-12-01

    Full Text Available En este trabajo se muestra un conjunto de resultados que permiten abordar el problema de la estabilización robusta de una familia de sistemas lineales positivos. Se describen dos nuevas aplicaciones en la teoría de control positivo para sistemas compartimentales y se muestra la aplicación de un teorema de robustez para tales sistemas

  18. ENEA presenta sistema ADS per trattamento scorie

    CERN Multimedia

    2002-01-01

    "Arriva il sistema Ads (Accelerator driven system) per il trattamento dei rifiuti radioattivi. Lo studio di fattibilita' per l' innovativa tecnologia, realizzato da un gruppo di lavoro congiunto Enea, Cea, Cern e Ansaldo, e' stato presentato oggi nel corso di un workshop presieduto dal premio Nobel per la fisica, Carlo Rubbia" (1/2 page).

  19. El Sistema and American Music Education

    Science.gov (United States)

    Lesniak, Melissa

    2012-01-01

    The brainchild of Jose Antonio Abreu, El Sistema, a music education program for aspiring orchestra musicians launched in Venezuela for students of limited means and now spreading to other parts of the world, has become a subject of interest to music teachers and teacher educators in North America. This article examines a bit of the program's…

  20. Elementos del sistema electoral del Congreso Mexicano

    Directory of Open Access Journals (Sweden)

    Aldo Medina García

    2013-08-01

    Full Text Available El presente artículo es un estudio de los componentes del sistema electoral, diseñado por el legislador mexicano para integrar al parlamento federal. Estudio desarrollado a sabiendas de la importancia que reviste el sistema electoral como un medio que permite a los ciudadanos convertir su voto expresado en las urnas en escaños a favor de determinado candidato o partido. En dicho análisis tomamos en cuenta la evolución constitucional del sistema electoral mexicano, la participación de las fuerzas minoritarias en la integración del Senado y de la Cámara de Diputados, la división que se hace del territorio en circunscripciones electorales, la barrera electoral que se fija a los partidos políticos para acceder a la representación en el Parlamento, la fórmula de asignación que se aplica a la votación obtenida, para finalmente concluir que el sistema electoral mexicano es esencialmente mayoritario aun cuando se vista de proporcional.

  1. EMMPRIN reduction via scFv-M6-1B9 intrabody affects α3β1-integrin and MCT1 functions and results in suppression of progressive phenotype in the colorectal cancer cell line Caco-2.

    Science.gov (United States)

    Sangboonruang, S; Thammasit, P; Intasai, N; Kasinrerk, W; Tayapiwatana, C; Tragoolpua, K

    2014-06-01

    Extracellular matrix metalloproteinase inducer (EMMPRIN) exhibits overexpression in various cancers and promotes cancer progression and metastasis via the interaction with its associated molecules. The scFv-M6-1B9 intrabody has a potential ability to reduce EMMPRIN cell surface expression. However, the subsequent effect of scFv-M6-1B9 intrabody-mediated EMMPRIN abatement on its related molecules, α3β1-integrin, MCT1, MMP-2 and MMP-9, is undefined. Our results demonstrated that the scFv-M6-1B9 intrabody efficiently decreased α3β1-integrin cell surface expression levels. In addition, intracellular accumulation of MCT1 and lactate were increased. These results lead to suppression of features characteristic for tumor progression, including cell migration, proliferation and invasion, in a colorectal cancer cell line (Caco-2) although there was no difference in MMP expression. Thus, EMMPRIN represents an attractive target molecule for the disruption of cancer proliferation and metastasis. An scFv-M6-1B9 intrabody-based approach could be relevant for cancer gene therapy.

  2. tavg3_3d_chm_Fv: MERRA Chem 3D IAU States Cloud Precip, Time average 3-hourly 1.25 x 1.25 degree V5.2.0 (MAT3FVCHM) at GES DISC

    Data.gov (United States)

    National Aeronautics and Space Administration — The MAT3FVCHM or tavg3_3d_chm_Fv data product is the MERRA Data Assimilation System Chemistry 3-Dimensional chemistry on layers file that is time averaged, 3D model...

  3. Observadores Distribuidos Garantistas para Sistemas en Red

    Directory of Open Access Journals (Sweden)

    Ramón A. García

    2017-07-01

    Full Text Available Resumen: En este artículo se propone un observador distribuido garantista para sistemas en red, considerando de forma explícita el problema de los retardos variables en las comunicaciones. Se asume que la información intercambiada entre agentes llega siempre a su destino, si bien las comunicaciones están sujetas a retardos variables, cuyo valor máximo se supone conocido. Cada observador trabaja con información parcial, y necesita comunicarse con observadores vecinos para llevar a cabo una estimación del estado completo del sistema. Para representar a los conjuntos garantistas, cuya función es acotar en tiempo real la región en la que se encuentra el estado del sistema, se ha optado por la utilización de zonotopos. Esto permite integrar de forma sencilla la información recibida por cada agente. Finalmente se presentan resultados de simulación para validar el algoritmo propuesto. Abstract: This paper proposes a guaranteed distributed observer for networked systems, taking into account the problem of the variable delays in communications. We assume that the information exchanged among the agents always arrives to its destination, although the communication are subject to variable delays, whose maximum value is known. Each observer works with partial information, and needs to communicate with neighbouring observers to carry out an estimation of the complete state of the system. The guaranteed sets, whose function is to delimit in real time the region in which the state of the system belong to, are represented by zonotopes. This kind of sets allows a simple integration of the information received by each agent. Finally some results obtained with the proposed algorithm are shown in simulations. Palabras clave: Estimación Distribuida, Observadores de Estado, Sistemas con Retardos, Sistemas en red, Zonotopos, Keywords: Distributed Estimation, State Observers, Delays Systems, Networked systems, Zonotopes

  4. Sistema de radiació solar via USB

    OpenAIRE

    Villanueva López, José Vicente

    2008-01-01

    L’objectiu d’aquest projecte és implementar un sistema de mesura de la irradiació solar a la Terra basat en una placa solar fotovoltaica. S’ha dissenyat i construït un sistema de mesura de la radiació solar que pot ser útil per aquells que instal·len sistemes fotovoltaics, que pot permetre l’estudi pràctic de les variables energètiques i econòmiques que ens poden indicar quin sistema és mes rentable construir, si un sistema de pannell solar fix, un sistema de pannell solar o...

  5. Sistema inteligente de detección de intrusiones

    OpenAIRE

    Martínez Puentes, Javier

    2011-01-01

    El sistema diseñado es un sistema de detección de intrusiones mediante el análisis del payload del tráfico de la red en busca de algún tipo de malware. Este sistema implementa su algoritmo de detección como “preprocesador dinámico” de Snort. Mediante el trabajo conjunto de Snort y del Sistema diseñado puede afirmarse que se obtiene un sistema altamente eficaz ante ataques conocidos (mediante el paso de reglas de Snort) e igualmente eficaz ante ataques nuevos o desconocidos (que era el obj...

  6. Stability engineering of anti-EGFR scFv antibodies by rational design of a lambda-to-kappa swap of the VL framework using a structure-guided approach.

    Science.gov (United States)

    Lehmann, Andreas; Wixted, Josephine H F; Shapovalov, Maxim V; Roder, Heinrich; Dunbrack, Roland L; Robinson, Matthew K

    2015-01-01

    Phage-display technology facilitates rapid selection of antigen-specific single-chain variable fragment (scFv) antibodies from large recombinant libraries. ScFv antibodies, composed of a VH and VL domain, are readily engineered into multimeric formats for the development of diagnostics and targeted therapies. However, the recombinant nature of the selection strategy can result in VH and VL domains with sub-optimal biophysical properties, such as reduced thermodynamic stability and enhanced aggregation propensity, which lead to poor production and limited application. We found that the C10 anti-epidermal growth factor receptor (EGFR) scFv, and its affinity mutant, P2224, exhibit weak production from E. coli. Interestingly, these scFv contain a fusion of lambda3 and lambda1 V-region (LV3 and LV1) genes, most likely the result of a PCR aberration during library construction. To enhance the biophysical properties of these scFvs, we utilized a structure-based approach to replace and redesign the pre-existing framework of the VL domain to one that best pairs with the existing VH. We describe a method to exchange lambda sequences with a more stable kappa3 framework (KV3) within the VL domain that incorporates the original lambda DE-loop. The resulting scFvs, C10KV3_LV1DE and P2224KV3_LV1DE, are more thermodynamically stable and easier to produce from bacterial culture. Additionally, C10KV3_LV1DE and P2224KV3_LV1DE retain binding affinity to EGFR, suggesting that such a dramatic framework swap does not significantly affect scFv binding. We provide here a novel strategy for redesigning the light chain of problematic scFvs to enhance their stability and therapeutic applicability.

  7. Sistema de inferencia difusa basado en relaciones Booleanas

    Directory of Open Access Journals (Sweden)

    Helbert Eduardo Espitia Cuchango

    2010-09-01

    Full Text Available Este documento describe la estructura de un sistema de inferencia difusa basado en relaciones booleanas. La teoría relacionada con lógica y conjuntos booleanos es una buena herramienta para el diseño de automatismos y sistemas digitales. Una variación con la cual se busca mejorar los sistemas basados en automatismos consiste en emplear conjuntos difusos en lugar de booleanos. Lo anterior se realiza con el objetivo de tener una acción continua en el actuador del automatismo. Al realizar esta variación y al aplicar la metodología de diseño de los sistemas de automatismos, aparecen los sistemas de inferencia difusa basados en relaciones booleanas.Aunque inicialmente esta propuesta se realizó considerando sistemas de automatismos, se observa que es posible extenderla a sistemas de inferencia difusa.

  8. Sistemas de tubagem de PPR reforçado com fibra de vidro destinados à distribuição de água quente e fria e a sistemas de aquecimento e de extinção automática de incêndio por água com aspersores no interior de edifícios - Critério para avaliação da aptidão ao uso

    OpenAIRE

    Real, L. P.

    2016-01-01

    Nesta publicação apresenta-se o critério presentemente seguido pelo LNEC para concessão de homologação com certificação, bem como para o posterior controlo periódico da qualidade a sistemas de tubagem de copolímero de polipropileno estatístico (“random”) reforçado com fibra de vidro na camada intermédia (PPR-FV-PPR), destinados à distribuição de água quente e fria, ao aquecimento e a redes de extinção automática de incêndio por água. Projeto ECOPOL / NÃO CONFIDENCIAL DED/NAICI...

  9. Sistemas electorales y justicia electoral a debate

    Directory of Open Access Journals (Sweden)

    Dieter Nohlen

    2015-07-01

    Full Text Available Despliega conceptos y planteamientos en torno a los sistemas electorales y la justicia electoral. El autor inicia con una descripción estricta de los sistemas electorales para orientar, en una segunda parte, un debate sobre la justicia electoral, que marca dos tendencias: la judicialización de la política y la politización de la administración y la jurisdicción electoral. Concluye que el desarrollo de la justicia electoral en América Latina, en sus múltiples dimensiones, es comprensible sólo con referencia al contexto sociocultural y político de la región

  10. Sistema de seguridad en redes locales utilizando sistemas multiagentes distribuidos. Net-Mass

    Directory of Open Access Journals (Sweden)

    Daniel Horfan Álvarez

    2005-01-01

    Full Text Available La seguridad en las redes de datos es uno de los elementos neurálgicos dentro de una organización, ya que requiere de diferentes estrategias de protección para salvaguardar puntos vulnerables, y de la coordinación y distribución de esfuerzos para cercar todas las posibles formas de ataques informáticos. En este artículo se propone un sistema multiagente distribuido como herramienta de protección para redes con diferentes sistemas operativos y susceptibilidad de diversos ataques.El sistema propuesto está conformado por un grupo de agentes autónomos heterogéneos con características particulares en cuanto a sus estrategias de detección de intrusos y protección de los sistemas primordiales de la red, se tiene planeado que estos agentes basen sus decisiones en la aplicación de diferentes técnicas de inteligencia artificial como heurística de sistemas expertos, algoritmos evolutivos y redes neuronales.

  11. Preclinical evaluation of multistep targeting of diasialoganglioside GD2 using a IgG-scFv bispecific antibody with high affinity for GD2 and DOTA metal complex

    Science.gov (United States)

    Cheal, Sarah M.; Xu, Hong; Guo, Hong-fen; Zanzonico, Pat B.; Larson, Steven M.; Cheung, Nai-Kong

    2014-01-01

    Bispecific antibodies (BsAb) have proven to be useful targeting vectors for pretargeted radioimmunotherapy (PRIT). We sought to overcome key PRIT limitations such as high renal radiation exposure and immunogenicity (e.g. of streptavidin-antibody fusions), to advance clinical translation of this PRIT strategy for diasialoganglioside GD2-positive (GD2(+)) tumors. For this purpose, a IgG-scFv BsAb was engineered using the sequences for the anti-GD2 humanized monoclonal antibody hu3F8 (1) and C825, a murine scFv antibody with high affinity for the chelator 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) complexed with beta-particle emitting radiometals such as 177Lu and 90Y (2, 3). A three-step regimen including hu3F8-C825, a dextran-based clearing agent, and p-aminobenzyl-DOTA radiolabeled with 177Lu (as 177Lu-DOTA-Bn; t1/2 = 6.71 days (d)) was optimized in immunocompromised mice carrying subcutaneous (s.c.) human GD2(+) neuroblastoma (NB) xenografts. Absorbed doses for tumor and normal tissues were ∼85 cGy/MBq and ≤3.7 cGy/MBq, respectively, with therapeutic indicies (TI) of 142 for blood and 23 for kidney. A therapy study (n = 5 per group; tumor volume: 240 ± 160 mm3) with three successive PRIT cycles (total 177Lu: ∼33 MBq; tumor dose ∼3400 cGy), revealed complete tumor response in 5/5 animals, with no recurrence up to 28 d post-treatment. Tumor ablation was confirmed histologically in 4/5 mice, and normal organs showed minimal overall toxicities. All non-treated mice required sacrifice within 12 d (>1.0 cm3 tumor volume). We conclude that this novel anti-GD2 PRIT approach has sufficient TI to successfully ablate s.c. GD2(+)–NB in mice while sparing kidney and bone marrow. PMID:24944121

  12. Sistema das Artes Visuais no Brasil

    Directory of Open Access Journals (Sweden)

    Nei Vargas da Rosa

    2017-10-01

    Full Text Available O Dossiê Sistema das Artes Visuais no Brasil se oferece como um profícuo dispositivo de análise do sistema da arte e de seu crescimento e avanço como objeto de reflexão e investigação. Reúne um grupo de pesquisadoras e pesquisadores que respondem por estudos de temas diversos, como a compreensão dos processos de valoração, considerando seus aspectos simbólicos e mercadológicos; a discussão sobre a gestão dos aparelhos culturais em meio ao receituário da política econômica centrada no mercado; a inserção recente de instrumentos de visibilidade e circulação da arte, como as ferias de arte e a Internet; o papel de colecionadores e colecionadoras no sistema de arte; entre outros assuntos; e os estudos sobre educação em museu de arte. Assim, o Dossiê segue uma ordenação que busca oferecer uma lógica conceitual e factual para compreensão do tema proposto, não excluindo a possibilidade de leitura direta a textos que ofereçam conteúdo de interesses específicos dos leitores e leitoras.

  13. Control integral systems; Sistemas integrales de control

    Energy Technology Data Exchange (ETDEWEB)

    Burgos, Estrella [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1999-12-31

    Almost two third of the electric power generation in Mexico are obtained from hydrocarbons, for that reasons Comision Federal de Electricidad (CFE) dedicated special commitment in modernizing the operation of fossil fuel central stations. In attaining this objective the control systems play a fundamental roll, from them depend a good share of the reliability and the efficiency of the electric power generation process, as well as the extension of the equipment useful life. Since 1984 the Instituto de Investigaciones Electricas (IIE) has been working, upon the request of CFE, on the development of digital control systems. To date it has designed and implemented a logic control system for gas burners, which controls 32 burners of the Unit 4 boiler of the Generation Central of Valle de Mexico and two systems for distributed control for two combined cycle central stations, which are: Dos Bocas, Veracruz Combined cycle central, and Gomez Palacio, Durango combined cycle central. With these two developments the IIE enters the World tendency of implementing distributed control systems for the fossil fuel power central update [Espanol] Casi las dos terceras partes de la generacion electrica en Mexico se obtienen a partir de hidrocarburos, es por eso que la Comision Federal de Electricidad (CFE) puso especial empeno en modernizar la operacion de las centrales termoelectricas de combustibles fosiles. En el logro de este objetivo los sistemas de control desempenan un papel fundamental, de ellos depende una buena parte la confiabilidad y la eficiencia en el proceso de generacion de energia electrica, asi como la prolongacion de la vida util de los equipos. Desde 1984 el Instituto de Investigaciones Electricas (IIE) ha trabajado, a solicitud de la CFE, en el desarrollo de sistemas digitales de control. A la fecha se han disenado e implantado un sistema de control logico de quemadores de gas, el cual controla 32 quemadores de la caldera de la unidad 4 de la central de generacion

  14. Control integral systems; Sistemas integrales de control

    Energy Technology Data Exchange (ETDEWEB)

    Burgos, Estrella [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1998-12-31

    Almost two third of the electric power generation in Mexico are obtained from hydrocarbons, for that reasons Comision Federal de Electricidad (CFE) dedicated special commitment in modernizing the operation of fossil fuel central stations. In attaining this objective the control systems play a fundamental roll, from them depend a good share of the reliability and the efficiency of the electric power generation process, as well as the extension of the equipment useful life. Since 1984 the Instituto de Investigaciones Electricas (IIE) has been working, upon the request of CFE, on the development of digital control systems. To date it has designed and implemented a logic control system for gas burners, which controls 32 burners of the Unit 4 boiler of the Generation Central of Valle de Mexico and two systems for distributed control for two combined cycle central stations, which are: Dos Bocas, Veracruz Combined cycle central, and Gomez Palacio, Durango combined cycle central. With these two developments the IIE enters the World tendency of implementing distributed control systems for the fossil fuel power central update [Espanol] Casi las dos terceras partes de la generacion electrica en Mexico se obtienen a partir de hidrocarburos, es por eso que la Comision Federal de Electricidad (CFE) puso especial empeno en modernizar la operacion de las centrales termoelectricas de combustibles fosiles. En el logro de este objetivo los sistemas de control desempenan un papel fundamental, de ellos depende una buena parte la confiabilidad y la eficiencia en el proceso de generacion de energia electrica, asi como la prolongacion de la vida util de los equipos. Desde 1984 el Instituto de Investigaciones Electricas (IIE) ha trabajado, a solicitud de la CFE, en el desarrollo de sistemas digitales de control. A la fecha se han disenado e implantado un sistema de control logico de quemadores de gas, el cual controla 32 quemadores de la caldera de la unidad 4 de la central de generacion

  15. Leaf gas exchange, fv/fm ratio, ion content and growth conditions of the two moringa species under magnetic water treatment

    International Nuclear Information System (INIS)

    Hasan, M.M.; Alharby, H.F.; Hajar, A.; Hakeem, K.R.

    2017-01-01

    The current greenhouse experiment investigates the role of magnetic water on the two Moringa species (Moringa oleifera and Moringa peregrina). Both species were exposed to the magnetic field (30 mT). The magnetic water increased the plant height, leaf number, leaflet number, and internode distances in both the species, respectively. Relative water content (RWC) and leaf area in both the species showed changes under magnetic water treatment. The results showed in magnetic water treatment, the leaf gas exchange parameters such as assimilation (A), stomatal conductance (gs), transpiration rate (E), and vapor pressure deficit (VPD) were increased. Similarly, Photosynthetic pigments (Chl a, Chl b, Chl (a+b), Carotenoids), photosynthetic water use efficiency (WUE) were also increased significantly. Magnetized water had also significant effects on the maximal efficiency of PSII photochemistry (Fv/Fm). Our study suggested that magnetic water treatment could be used as an environment-friendly technology for improving the growth and physiology of Moringa species. In addition, this technology could be further incorporated into the traditional methods of agriculture for the improvement of crop plants, particularly in the arid and sub-arid areas of the world. (author)

  16. The use of a cocktail of single chain Fv antibody fragments to improve the in vitro and in vivo targeting of melanoma

    International Nuclear Information System (INIS)

    Pacifico, M.D.; Pearl, R.A.; Kupsch, J.M.

    2006-01-01

    Radio scintigraphy using single chain antibody fragments (scFvs) offers a potenti al means of early detection of melanoma metastases. However, previous studies have shown suboptimal levels of tumour localization and nonspecific background accumulation which may be due to antigen heterogeneity. We aimed to improve tumour localization by using a cocktail of different scFvs targeting different epitopes on melanoma cells. We have previously developed three scFvs against distinct and highly tumour-specific melanoma cell-surface antigens by chain shuffling and antibody phage selection on melanoma cells. Three scFvs, RAFT3, B3 and B4 were labeled with 1 25I odine and tested both individually and as a cocktail in a nude mouse xenograft model far human melanoma. Results demonstrated improved tumour localization in vivo when compared to the individual scFvs. Tumour uptake of the cocktail at l hour was 24.220% ID/g (injected dose/gram) compared with 2.854%, 2.263% and 1.355% far B4, RAFT3 and B3, respectively, when injected individually. In addition, the cocktail exhibited significantly superior tumour to normal tissue ratios far muscle and spleen (p<0.05). A combination or cocktail of scFv clones may have an advantage aver individual scFvs far melanoma targeting in patients because of heterogeneity in the expression of different epitopes of antigens on melanoma cells

  17. Evaluation of anti-HER2 scFv-conjugated PLGA–PEG nanoparticles on 3D tumor spheroids of BT474 and HCT116 cancer cells

    International Nuclear Information System (INIS)

    Le, Thi Thuy Duong; Pham, Thu Hong; Ngo, Thi Hong Giang; Le, Quang Huan; Nguyen, Trong Nghia; Hoang, Thi My Nhung

    2016-01-01

    Three-dimensional culture cells (spheroids) are one of the multicellular culture models that can be applied to anticancer chemotherapeutic development. Multicellular spheroids more closely mimic in vivo tumor-like patterns of physiologic environment and morphology. In previous research, we designed docetaxel-loaded pegylated poly(D, L-lactide-co-glycolide) nanoparticles conjugated with anti-HER2 single chain antibodies (scFv–Doc–PLGA–PEG) and evaluated them in 2D cell culture. In this study, we continuously evaluate the cellular uptake and cytotoxic effect of scFv–Doc–PLGA–PEG on a 3D tumor spheroid model of BT474 (HER2-overexpressing) and HCT116 (HER2-underexpressing) cancer cells. The results showed that the nanoparticle formulation conjugated with scFv had a significant internalization effect on the spheroids of HER2-overexpressing cancer cells as compared to the spheroids of HER2-underexpressing cancer cells. Therefore, cytotoxic effects of targeted nanoparticles decreased the size and increased necrotic score of HER2-overexpressing tumor spheroids. Thus, these scFv–Doc–PLGA–PEG nanoparticles have potential for active targeting for HER2-overexpressing cancer therapy. In addition, BT474 and HCT116 spheroids can be used as a tumor model for evaluation of targeting therapies. (paper)

  18. Targeted delivery of CD44s-siRNA by ScFv overcomes de novo resistance to cetuximab in triple negative breast cancer.

    Science.gov (United States)

    Fu, Wenyan; Sun, Hefen; Zhao, Yang; Chen, Mengting; Yang, Lipeng; Yang, Xueli; Jin, Wei

    2018-05-16

    The overexpression of EGFR often occurs in TNBC, and the anti-EGFR receptor antibody cetuximab is used widely to treat metastatic cancer in the clinic. However, EGFR-targeted therapies have been developed for TNBC without clinical success. In this study, we show that impaired EGFR degradation is crucial for resistance to cetuximab, which depends on the cell surface molecule CD44. To further investigate the role of CD44 in EGFR signaling and its treatment potential, we developed a targeting fusion protein composed of an anti-EGFR scFv generated from cetuximab and truncated protamine, called Ce-tP. CD44 siRNA can be specifically delivered into EGFR-positive TNBC cells by Ce-tP. Efficient knockdown of CD44 and suppression of both EGFR and downstream signaling by the Ce-tP/siRNA complex were observed in EGFR-positive TNBC cells. More importantly, our results also showed that targeted delivery of siRNA specific for CD44 can efficiently overcome resistance to EGFR targeting in TNBC cells both in vitro and in vivo. Overall, our results establish a new principle to achieve EGFR inhibition in TNBC and limit drug resistance. Copyright © 2018 Elsevier Ltd. All rights reserved.

  19. Importancia del manual de los sistemas contables en el sistema de control interno.

    OpenAIRE

    Nicolás Valdés Rodríguez

    2007-01-01

    La dirección administrativa del país en los últimos 3 años ha insistido en la necesidad de profundizar en los mecanismos de control económico –financiero de las entidades, el presente trabajo va dirigido a contribuir en alguna medida a lograr ese propósito. El sistema contable de una Entidad forma parte indisoluble del Sistema de Control Interno de la misma, por la vinculación tan estrecha que existe entre los mismos. El objetivo fundamental del trabajo ”Importancia del Manual de ...

  20. Del Actual Sistema de Enfermedad a un verdadero Sistema de Salud

    Directory of Open Access Journals (Sweden)

    Lidice Alvarez-Miño

    2018-01-01

    Full Text Available Esta editorial tiene como objetivo generar una reflexión y abrir el debate sobre el sistema actual de salud que se refleja en prácticas naturalizadas  que se contradicen con la esencia del significado de la salud. Se trata de la costumbre de acudir al sistema de salud únicamente cuando se considera que se está enfermo, o peor aún, la equivalencia reduccionista y casi automática del concepto de salud como sinónimo de medicina.

  1. Preliminary analysis for implementation of a hybrid solar-wind system with storage of electrical energy generated through electrolytic hydrogen and fuel cells; Analise preliminar para implementacao de um sistema hibrido solar-eolico com armazenamento da energia eletrica gerada atraves de hidrogenio eletrolitico e celula a combustivel

    Energy Technology Data Exchange (ETDEWEB)

    Lopes, Daniel Gabriel; Furlan, Andre Luis; Lopes, Davi Gabriel [Universidade Estadual de Campinas (FEM/UNICAMP), SP (Brazil). Fac. de Engenharia Mecanica], e-mail: danielg@fem.unicamp.br; Silva, Ennio Peres; Apolinario, Fernando Resende [Universidade Estadual de Campinas (IFGW/UNICAMP), SP (Brazil). Inst. de Fisica Gleb Wataghin. Lab. de Hidrogenio; Silva, Maria Eugenia Vieira da; Rocha, Paulo Alexandre Costa [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil). Dept. de Engenharia Mecanica e de Producao; Codeceira Neto, Alcides [Companhia Hidro Eletrica do Sao Francisco (CHESF), Recife, PE (Brazil)

    2008-07-01

    Due to relevance of the study and applicability of hybrid electric power generation from solar photovoltaic and wind power in Brazil, the present paper aims to introduce briefly the importance of technical and economic comparison of two electrical energy storage technologies (batteries versus H2 + fuel cell) and also to indicate the previous difficulties related to this possible application. In this context, it was intended a partnership between State University of Campinas - UNICAMP, the Federal University of Ceara - UFC and Hydro Electric Company of the Sao Francisco - CHESF in the meaning of makes viable an implementation. (author)

  2. Development of a controller based on Fuzzy theory to better use the energy of a hybrid system power generation solar-photovoltaic and wind; Desenvolvimento de um controlador baseado na teoria Fuzzy para melhor aproveitamento da energia de um sistema hibrido de geracao de energia solar-fotovoltaico e eolico

    Energy Technology Data Exchange (ETDEWEB)

    Caneppele, Fernando de Lima [Universidade Estadual Paulista (UNESP), Itapeva, SP (Brazil). Campus Experimental], E-mail: fernando@itapeva.unesp.br; Seraphim, Odivaldo Jose [Universidade Estadual Paulista (FCA/UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas. Dept. de Engenharia Rural; Gabriel Filho, Luis Roberto de Almeida [Universidade Estadual Paulista (UNESP), Tupa, SP (Brazil). Campus Experimental

    2010-07-01

    The work developed a methodology fuzzy and simulated its use in control of a hybrid system of electric power generation, using solar-photovoltaic and wind energy. Using this control system, we get the point of maximum energy generation and transfer all the energy generated from alternative sources, solar-photovoltaic and wind energy to charge and / or batteries. The model uses three input variables, which are: wind (wind speed), sun (solar radiation) and batteries (charge the battery bank). With these variables, the fuzzy system will play, according to the rules to be described, what is the source of power supply system, which will have priority and how the batteries are loaded. For the simulations regarding the use of fuzzy theory to control, we used the scientific computing environment MATLAB. In this environment have been analyzed and simulated all mathematical modeling, rules and other variables described in the fuzzy system. This model can be applied to implement a control system of hybrid power generation, providing the best use of renewable energy, solar and wind, so that we can extract the maximum possible energy of these alternative sources without compromising the environment. (author)

  3. The provision of electricity through a photovoltaic-wind hybrid system in an isolated community in the Jalapao, municipality of Mateiros, state of Tocantins, Brazil; A disponibilizacao de energia eletrica atraves de um sistema hibrido eolico-fotovoltaico em uma comunidade isolada na regiao do Jalapao, municipio de Mateiros, estado do Tocantins

    Energy Technology Data Exchange (ETDEWEB)

    Zukowski Junior, J.C.; Santos, W.F.; Nobrega, S.L. de; Marcon, R.O. [Universidade Luterana do Brasil (CEULP/ULBRA), Palmas, TO (Brazil). Centro Universitario Luterano de Palmas. Curso de Engenharia Agricola], Emails: zukowski@uft.edu.br, olavo@ulbra-to.br, silvestre@ulbra-to.br

    2009-07-01

    Nowadays Brazil has many isolated communities. This isolation occurs mainly due to territorial conditions. Besides this aspect, the lack of structure produces another isolation, much more harmful, the social one. To promote social inclusion and sustainable development, some requirements are essential. Amongst all of them, the provision of electricity is the most prominent. This study aimed to provide electricity to an isolated community in the Jalapao region, Mateiros city, in the state of Tocantins. It was installed a hybrid aeolian-photovoltaic system, with capacity to produce 9676.80 kWh per year. This electricity system aims to improve the production process, so it was installed at the local community workshop. The results indicate that the system can be sustainable if the community makes use of the electricity generation, using it in the production process. (author)

  4. Economic and financial analysis of hybrid solar-wind power generation system installed in a isolated community in Jalapao-Tocantins, Brazil; Analise economica-financeira do sistema de geracao de energia eletrica hibrido solar-eolico instalado em uma comunidade isolada no Jalapao-TO

    Energy Technology Data Exchange (ETDEWEB)

    Santos, W.F.; Zukowski Junior, J.C.; Nobrega, S.L. de; Marcon, R.O. [Universidade Luterana do Brasil (CEULP/ULBRA), Palmas, TO (Brazil). Centro Universitario Luterano de Palmas. Curso de Engenharia Agricola], Emails: zukowski@uft.edu.br, olavo@ulbra-to.br, silvestre@ulbra-to.br

    2009-07-01

    With the increasing of energetic demand in several production sectors, the search for renewable energy sources which do not cause negative environmental impacts has become more and more relevant. Amongst all the important factors to decide for installing isolated generation systems as renewable sources or diesel and taking the distribution network to isolated communities, it should be considered the economic viability of those benefits. This study aimed to analyse the hybrid solar wind system installed in an isolated community, in Jalapao - TO. Three scenarios were analyzed: first of them, getting the capital back with MRI = 6% per year; second one, getting the capital back with MRI = 0; and third, without getting the investment back. In all the three cases, O and M costs were considered. The proceeds were calculated from the production at the communities workshop, in which the electricity was available. The results indicated that this technology is economically viable if the generated energy is used for production process. It also demonstrated that the third scenario is more interesting for the community, since the contribution per family is only 8% of its incomes. (author)

  5. Analysis of economic viability in a hybrid solar-wind system in the generation of electrical energy to an Maroon isolated community of Jalapao - TO, Brazil; Analise da viabilidade economica em um sistema hibrido solar-eolico na geracao de energia eletrica para uma comunidade isolada e quilombola do Jalapao - TO

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Weder Ferreira dos; Zukowski Junior, Joel Carlos; Nobrega, Silvestre Lopes da; Marcon, Rogerio Olavo [Universidade Luterana do Brasil (CEULP/ULBRA), Palmas, TO (Brazil). Centro Universitario Luterano

    2008-07-01

    With the increasing of energetic demand in several production sectors, the search for renewable energy sources which do not cause negative environmental impacts has become more and more relevant. Amongst all the important factors to decide for installing isolated generation systems as renewable sources or diesel and taking the distribution network to isolated communities, it should be considered the economic viability of those benefits. This study aimed to analyse the hybrid solar-aeolian system installed in an isolated community, in Jalapao/TO. Three scenarios were analyzed: first of them, getting the capital back with MRI = 6% per year; second one, getting the capital back with MRI = 0; and third, without getting the investment back. In all the three cases, O and M costs were considered. The proceeds were calculated from the production at the community workshop, in which the electricity was available. The results indicated that this technology is economically viable if the generated energy is used for production process. It also demonstrated that the third scenario is more interesting for the community, since the contribution per family is only 8% of its incomes. (author)

  6. CAPHIGAS Project: Design of a Novel WGS-Adsorbent-Membrane Hybrid System for the Simultaneous Capture of CO{sub 2} and Production of H2 (Ref.: Ene2009-08002); Proyecto CAPHIGAS: Diseno de un Sistema Hibrido WGS-Adsorbente-Membrana para la Captura de CO{sub 2} con Produccion de H{sub 2} (Ref: Ene2009-08002)

    Energy Technology Data Exchange (ETDEWEB)

    Marano, M.; Barreiro, M. M.; Sanchez, J. M.

    2014-02-01

    This report describes the general objective, tasks and main results and conclusions drawn within CAPHIGAS Project, Plan Nacional de I+D+I 2008-2011, financed by the Spanish Ministry of Science and Innovation and carried out by the Valorization of Fuels and Wastes Group of Ciemat. The general objective of the project was the design and development of a novel hybrid system for the simultaneous removal of CO{sub 2} and production of H{sub 2} using a WGS catalyst-adsorbent membrane configuration. The novel system proposed has provided new insight into the adsorption and reaction processes and has allowed an optimization of the operating conditions to take advantage of the synergies between both processes. In this report main future activities are also reported. (Author)

  7. Caos en sistemas clásico-cuánticos

    Science.gov (United States)

    Dirani, L. D.; Núñez, J. A.

    Una de las formas de estudiar la existencia de caos en el comportamiento de un sistema clásico-cuántico, es truncando el espacio de Hilbert del sistema cuántico. El objetivo de este trabajo es analizar como afecta dicho truncamiento en la dinámica del sistema. Para tal fin se propone un Hamiltoniano integrable, cuyos valores de expectación asociados a coordenadas y momentos cuánticos responden, por el teorema de Ehrenfest, a un sistema de dos osciladores clásicos con acoplamiento lineal.

  8. Um modelo de enfermagem como sistema complexo adaptativo

    Directory of Open Access Journals (Sweden)

    Mariana Vieira

    2009-01-01

    Full Text Available El surgimiento y el uso de los sistemas complejos adaptativos suplieron la necesidad de contar con una nueva alternativa, recurriendo a los paradigmas existentes. En cuanto al sistema de salud y de enfermería, ambos pueden verse como sistemas complejos adaptativos al aplicar un modelo visual que debe ser explorado para potenciar la complejidad de la ciencia de enfermería y de cuidados de salud. Desde esta perspectiva, el/la enfermero/a es un sistema complejo adaptativo, dinámico que interactúa, pero también es agente de un sistema complejo adaptativo en una unidad de enfermería, que a la vez es un sistema complejo adaptativo en una organización de la salud. Hoy en día la/el profesional de enfermería busca actualizarse y cualificarse en diversas especialidades, que van desde enfermería neonatal hasta cuidados geriátricos, para ejecutar sus actividades y visualizar su ambiente laboral desde la perspectiva de un sistema complejo. En consecuencia, la organización del sistema de cuidados permite, mediante los sistemas complejos, apoyarse en el saber compartido de los diversos profesionales y en el trabajo en equipo para disfrutar la complicidad de la cadena cliente-usuario-profesionales.

  9. Sistema fotovoltaico de iluminación solar

    OpenAIRE

    Víctor Hugo Benítez Baltazar; Guadalupe Araceli Torres Valverde; Luis Armando Gámez Valdéz; Jesús Horacio Pacheco Ramírez

    2013-01-01

    El artículo es el resultado de un trabajo de investigación enfocado al uso de sistemas fotovoltaicos para la iluminación por medio de diodos emisores de luz tipo LED. Se busca reducir el consumo energético mediante la implementación de un sistema de iluminación basado en LED hasta en un 80%, donde la fuente primaria de energía es el Sol. Se demuestra la viabilidad de los sistemas solares de iluminación LED mediante la construcción de un prototipo. Los sistemas de iluminación fotovoltaicos son...

  10. Evaluation of an anti-p185{sup HER2} (scFv-C{sub H}2-C{sub H}3){sub 2} fragment following radioiodination using two different residualizing labels: SGMIB and IB-Mal-D-GEEEK

    Energy Technology Data Exchange (ETDEWEB)

    Vaidyanathan, Ganesan [Duke University Medical Center, Durham, NC 27710 (United States)], E-mail: ganesan.v@duke.edu; Jestin, Emmanuelle [Duke University Medical Center, Durham, NC 27710 (United States); Olafsen, Tove; Wu, Anna M. [Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, CA 90095 (United States); Zalutsky, Michael R. [Duke University Medical Center, Durham, NC 27710 (United States)

    2009-08-15

    Introduction: A 105-kDa double mutant single-chain Fv-Fc fragment (scFv-Fc DM) derived from the anti-p185{sup HER2} hu4D5v8 antibody (trastuzumab; Herceptin) has been described recently. The goal of this study was to investigate whether improved tumor targeting could be achieved with this fragment through the use of residualizing radioiodination methods. Methods: The scFv-Fc DM fragment was radioiodinated using N-succinimidyl 4-guanidinomethyl 3-[{sup 131}I]iodobenzoate ([{sup 131}I]SGMIB) and N{sup {epsilon}}-(3-[{sup 131}I]iodobenzoyl)-Lys{sup 5}-N{sup {alpha}}- maleimido-Gly{sup 1}-GEEEK ([{sup 131}I]IB-Mal-D-GEEEK), two residualizing radioiodination agents that have been used successfully with intact antibodies. Paired-label internalization assays of the labeled fragments were performed in vitro using MCF7 human breast cancer cells transfected to express HER2 (MCF7-HER2); comparisons were made to scFv-Fc DM directly radioiodinated using Iodogen. The tissue distribution of the scFv-Fc DM labeled with [{sup 125}I]IB-Mal-D-GEEEK and [{sup 131}I]SGMIB was compared in athymic mice bearing MCF7-HER2 xenografts. Results: The scFv-Fc DM fragment was labeled with [{sup 131}I]SGMIB and [{sup 131}I]IB-Mal-D-GEEEK in conjugation yields of 53% and 25%, respectively, with preservation of immunoreactivity for HER2. Internalization assays indicated that labeling via SGMIB resulted in a 1.6- to 3.5-fold higher (P<.05) retention of radioactivity, compared to that from the directly labeled fragment, in HER2-expressing cells during a 24-h observation period. Likewise, the amount of radioactivity retained in cells from the IB-Mal-D-GEEEK-labeled fragment was 1.4- to 3.3-fold higher (P<.05). Tumor uptake of radioiodine activity in athymic mice bearing MCF7-HER2 xenografts in vivo was significantly higher for the [{sup 125}I]IB-Mal-D-GEEEK-labeled scFv-Fc DM fragment compared with that of the [{sup 131}I]SGMIB-labeled fragment, particularly at later time points. The uptake of {sup

  11. El Sistema de patentes en Colombia

    Directory of Open Access Journals (Sweden)

    Rafael Viana Barceló

    2014-01-01

    Full Text Available En este documento se realiza un análisis económico del Sistema de Patentes de Colombia. Para ello, se establece el efecto que tienen las patentes concedidas a los inventores foráneos sobre la Inversión Extranjera Directa y el Producto Interno Bruto Nacional, a través de la técnica de regresión de data panel. De igual manera, se identifican los sectores económicos que registran mayores tasas de innovación por parte de los inventores nacionales y extranjeros. Se muestra que el sistema nacional de propiedad intelectual tiene un grado de fortaleza acorde con el resto de países latinoamericanos; para ello, se construye un índice de grado de fortalecimiento de sistema de patente nacional que luego será comparado con el de otros países latinos.Palabras Clave: Sistema de Patentes; Derechos de Propiedad Intelectual; Inversión Directa extranjera; Producto Interno Bruto e innovación tecnológica. The system of patents in ColombiaAbstractIn this document an economic analysis of the System of Patents of Colombia is made. For it, the effect that has the patents granted to the foreign inventors on the Direct Foreign Investment and the Internal Product Gross National, through the technique of regression of data settles down panel. Of equal way, the economic sectors are identified that register greater rates of innovation on the part of the national and foreign inventors.Sample that the national system of intellectual property has a degree of agreed strength with the rest of Latin American countries; for it, an index of degree of fortification of system of national patent is constructed that soon will be compared with the one of other Latin countries.Keywords: System Patent; Rights of Intellectual Property; Foreign Direct Investment; Gross Domestic Product and Technology Innovation.

  12. CAPACIDAD TRANSACCIONAL: EVIDENCIAS DEL SISTEMA FINANCIERO PERUANO

    OpenAIRE

    TELLO-GAMARRA, JORGE; HERNANI-MERINO, MARTÍN; LÍMACO-MAMANI, BRYAN IVÁN

    2017-01-01

    RESUMEN Aunque existen algunos esfuerzos por integrar las perspectivas de capacidades y la economía de los costos de transacción, los avances en esa dirección son escasos. La integración de esas dos perspectivas es una emergente área de estudio llamada capacidad transaccional. Este artículo tiene por objetivo examinar la capacidad transaccional de firmas que operan en el sistema financiero peruano. El método escogido para la búsqueda de evidencias en ese sector es el estudio de casos múltiple...

  13. Viabilidade financeira em sistema de aquaponia

    OpenAIRE

    Kodama, Goro

    2016-01-01

    O presente estudo avaliou uma propriedade particular do em torno de Brasília que utiliza o sistema de aquaponia numa escala pequena de produção. Foi utilizado o método Monte Carlo para estimar a sua viabilidade financeira e a probabilidade do volume de produção e consequentemente avaliar o risco desse empreendimento. Os dados analisados foram os custos fixos e variáveis, receitas e os indicadores de viabilidade financeira que são valor presente líquido (VPL), benefício periódico econômico (BP...

  14. Uvajanje sistema poslovne inteligence v koncernu

    OpenAIRE

    Menard, Nejc

    2016-01-01

    Skupaj z rastjo poslovnih sistemov raste tudi potreba po ustreznih informacijah o stanju znotraj sistema. V te namene so bili razviti sistemi poslovne inteligence. Ta širok pojem omogoča podjetjem ali organizacijam vpogled v delovanje njihovih kritičnih funkcij skozi orodja za poročila, analizo in vizualizacije. Na podlagi privzetega modela uvajanja informacijskih rešitev smo zapisali usklajene prilagoditve in jih preverili na primeru implementacije rešitve v koncernu Kolektor. S tem spec...

  15. DOS SISTEMAS AMBIENTAIS AO SISTEMA FLUVIAL – UMA REVISÃO DE CONCEITOS

    Directory of Open Access Journals (Sweden)

    Jonas Otaviano Praça de Souza

    2013-10-01

    Full Text Available Tentando consolidar as noções de totalidade, a geografia física passa a utilizar a noção de sistemas dinâmicos, não lineares e de comportamento caótico. Deste modo, a abordagem sistêmica/complexa apresenta-se como meio de compreensão do mundo natural, assim possibilitando a previsão de mudanças, avaliação dos sistemas ambientais, distúrbios e limiares do sistema. Nota-se, assim, a importância dos estudos processuais para o gerenciamento de recursos hídricos, tornando imprescindíveis estudos sistêmicos que analisem os diversos elementos importantes para a análise dos recursos hídricos, dentre eles as questões processuais. Deste modo, a necessidade de se trabalhar o gerenciamento integrado de recursos hídricos, enquadrado na perspectiva sistêmica, solidificou a bacia hidrográfica e/ou bacia de drenagem como recorte espacial sistêmico. Delimitada a área pode-se compreender a bacia hidrográfica como um Sistema Fluvial, conceituado por Schumm (1977, que é analisado a partir da zona fonte de sedimentos, da rede de transporte e dos sítios de deposição. Esses elementos não são espacialmente excludentes, interagindo entre si, além de apresentar escalas diferenciadas. Para o estudo do sistema fluvial é necessário levar em consideração quatro conceitos: uniformidade dos processos, limiares de mudança, evolução da paisagem e respostas complexas.

  16. Importancia del manual de los sistemas contables en el sistema de control interno.

    Directory of Open Access Journals (Sweden)

    Nicolás Valdés Rodríguez

    2007-01-01

    Full Text Available La dirección administrativa del país en los últimos 3 años ha insistido en la necesidad de profundizar en los mecanismos de control económico –financiero de las entidades, el presente trabajo va dirigido a contribuir en alguna medida a lograr ese propósito. El sistema contable de una Entidad forma parte indisoluble del Sistema de Control Interno de la misma, por la vinculación tan estrecha que existe entre los mismos. El objetivo fundamental del trabajo ”Importancia del Manual de los Sistemas Contables en el Sistema de Control Interno”, es destacar la utilidad práctica, técnica y organizativa que tiene el Manual para elevar la eficiencia del trabajo contable de las Entidades y su repercusión positiva en la eficiencia el sistema de Control Interno establecido. Los beneficios que aporta el Manual radican en que crea una base normativa contable adecuada a las características de cada entidad. Como se sabe en las transformaciones económicas realizadas en el país, la existencia de dos monedas en el trabajo económico-contable de muchas entidades, la puntualización del objeto social y la cartera de productos y servicios han complejizado el trabajo contable, requiriendo una mayor adecuación a las características propias de cada entidad, lo cual se logra con la realización del Manual.

  17. Construction of genetically engineered M13K07 helper phage for simultaneous phage display of gold binding peptide 1 and nuclear matrix protein 22 ScFv antibody.

    Science.gov (United States)

    Fatemi, Farnaz; Amini, Seyed Mohammad; Kharrazi, Sharmin; Rasaee, Mohammad Javad; Mazlomi, Mohammad Ali; Asadi-Ghalehni, Majid; Rajabibazl, Masoumeh; Sadroddiny, Esmaeil

    2017-11-01

    The most common techniques of antibody phage display are based on the use of M13 filamentous bacteriophages. This study introduces a new genetically engineered M13K07 helper phage displaying multiple copies of a known gold binding peptide on p8 coat proteins. The recombinant helper phages were used to rescue a phagemid vector encoding the p3 coat protein fused to the nuclear matrix protein 22 (NMP22) ScFv antibody. Transmission electron microscopy (TEM), UV-vis absorbance spectroscopy, and field emission scanning electron microscopy (FE-SEM) with energy dispersive X-ray spectroscopy (EDX) analysis revealed that the expression of gold binding peptide 1 (GBP1) on major coat protein p8 significantly enhances the gold-binding affinity of M13 phages. The recombinant bacteriophages at concentrations above 5×10 4 pfu/ml red-shifted the UV-vis absorbance spectra of gold nanoparticles (AuNPs); however, the surface plasmon resonance of gold nanoparticles was not changed by the wild type bacteriophages at concentrations up to 10 12 pfu/ml. The phage ELISA assay demonstrated the high affinity binding of bifunctional bacteriophages to NMP22 antigen at concentrations of 10 5 and 10 6 pfu/ml. Thus, the p3 end of the bifunctional bacteriophages would be able to bind to specific target antigen, while the AuNPs were assembled along the coat of virus for signal generation. Our results indicated that the complex of antigen-bacteriophages lead to UV-vis spectral changes of AuNPs and NMP22 antigen in concentration range of 10-80μg/ml can be detected by bifunctional bacteriophages at concentration of 10 4 pfu/ml. The ability of bifunctional bacteriophages to bind to antigen and generate signal at the same time, makes this approach applicable for identifying different antigens in immunoassay techniques. Copyright © 2017 Elsevier B.V. All rights reserved.

  18. Numerical study of steady/unsteady flow and heat transfer in porous media using a characteristics-based matrix-free implicit FV method on unstructured grids

    International Nuclear Information System (INIS)

    Chiem, Kok Siong; Zhao Yong

    2004-01-01

    In this study, a high-resolution characteristic-based finite-volume (FV) method on unstructured grids [Int. J. Numer. Method Eng. 50 (2001) 11; Int. J. Heat Fluid Flow 21 (2000) 432] is extended by a matrix-free implicit dual-time stepping scheme for the numerical simulation of steady and unsteady flow and heat transfer with porous media. The method has been used to study the characteristics of a complex problem: flow and heat transfer in a channel with multiple discrete porous blocks, which was originally proposed by Huang and Vafai [J. Thermophys. Heat Transfer 8 (3) (1994) 563]. In addition, flow and heat transfer in a channel partially or fully filled with porous layers and containing solid protruding blocks with constant heat flux on its lower surface are also investigated in details. Hydrodynamic and heat transfer results are reported for both steady and transient flow cases. In particular, the effects of Darcy and Reynolds numbers on heat transfer augmentation and pressure loss are studied. An in-depth discussion of the formation and variation of recirculation is presented and the existence of optimum porous insert is demonstrated. At high Reynolds numbers the flow in the porous channel exhibits a cyclic characteristics although unlike the non-porous channel flow, the cyclic vortex development is only restricted to a small area behind the last solid block, while temperature changes more slowly and does not exhibit cyclic variations over a long period of time. It is shown that for all the cases studied altering some parametric values can have significant and interesting effects on both flow pattern as well as heat transfer characteristics

  19. Crystal structure of the anti-(carcinoembryonic antigen) single-chain Fv antibody MFE-23 and a model for antigen binding based on intermolecular contacts.

    Science.gov (United States)

    Boehm, M K; Corper, A L; Wan, T; Sohi, M K; Sutton, B J; Thornton, J D; Keep, P A; Chester, K A; Begent, R H; Perkins, S J

    2000-03-01

    MFE-23 is the first single-chain Fv antibody molecule to be used in patients and is used to target colorectal cancer through its high affinity for carcinoembryonic antigen (CEA), a cell-surface member of the immunoglobulin superfamily. MFE-23 contains an N-terminal variable heavy-chain domain joined by a (Gly(4)Ser)(3) linker to a variable light-chain (V(L)) domain (kappa chain) with an 11-residue C-terminal Myc-tag. Its crystal structure was determined at 2.4 A resolution by molecular replacement with an R(cryst) of 19.0%. Five of the six antigen-binding loops, L1, L2, L3, H1 and H2, conformed to known canonical structures. The sixth loop, H3, displayed a unique structure, with a beta-hairpin loop and a bifurcated apex characterized by a buried Thr residue. In the crystal lattice, two MFE-23 molecules were associated back-to-back in a manner not seen before. The antigen-binding site displayed a large acidic region located mainly within the H2 loop and a large hydrophobic region within the H3 loop. Even though this structure is unliganded within the crystal, there is an unusually large region of contact between the H1, H2 and H3 loops and the beta-sheet of the V(L) domain of an adjacent molecule (strands DEBA) as a result of intermolecular packing. These interactions exhibited remarkably high surface and electrostatic complementarity. Of seven MFE-23 residues predicted to make contact with antigen, five participated in these lattice contacts, and this model for antigen binding is consistent with previously reported site-specific mutagenesis of MFE-23 and its effect on CEA binding.

  20. Sistemas de control push-pull. Un estudio comparativo.

    OpenAIRE

    Ruiz Usano, Rafael; Muñoz Pérez, Miguel Ángel

    2001-01-01

    Un aspecto fundamental a nivel operacional en los sistemas de producción es la obtención de la cantidad deseada de unidades a producir con la mínima cantidad de inventario en proceso. Para conseguirlo podemos optar por distintos sistemas de control de l

  1. Realidad de los sistemas de gestión ambiental

    Directory of Open Access Journals (Sweden)

    Sandra Constanza Escobar Cárdenas

    2009-07-01

    Full Text Available Este artículo presenta un análisis sobre la posibilidad de integrar los diferentes sistemas de gestión y la importancia que tiene los sistemas de gestión ambiental para el mejoramiento de las organizaciones. Luego de analizar comparativamente los modelos existentes, la autora presenta algunas recomendaciones de implementación.

  2. Teoría de sistemas (2008/09)

    OpenAIRE

    Pla López, Rafael

    2008-01-01

    Conceptos fundamentales de la Teoría general de sistemas. Nociones básicas de estadística. Modelos cibernéticos. Sistemas caóticos.Asignatura optativa de Ciencias Ambientales. Primer ciclo. Segundo curso. Cuatrimestral: 6 créditos.

  3. Reversible dimer formation and stability of the anti-tumour single-chain Fv antibody MFE-23 by neutron scattering, analytical ultracentrifugation, and NMR and FT-IR spectroscopy.

    Science.gov (United States)

    Lee, Yie Chia; Boehm, Mark K; Chester, Kerry A; Begent, Richard H J; Perkins, Stephen J

    2002-06-28

    MFE-23 is a single chain Fv (scFv) antibody molecule used to target colorectal cancer through its high affinity for the tumour marker carcinoembryonic antigen (CEA). ScFv molecules are formed from peptide-linked antibody V(H) and V(L) domains, and many of these form dimers. Our recent crystal structure for MFE-23 showed that this formed an unusual symmetric back-to-back association of two monomers that is consistent with a domain-swapped diabody structure. Neutron scattering and modelling fits showed that MFE-23 existed as compact V(H)-V(L)-linked monomers at therapeutically relevant concentrations below 1 mg/ml. Size-exclusion gel chromatography showed that the monomeric and dimeric forms of MFE-23 could be separated, and that the proportions of these two forms depended on the starting MFE-23 concentration. Sedimentation equilibrium experiments by analytical ultracentrifugation at nine concentrations of MFE-23 indicated a reversible monomer-dimer self-association equilibrium with an association constant of 1.9x10(3)-2.2x10(3) M(-1). Sedimentation velocity experiments using the time derivative g(s(*)) method showed that MFE-23-His has a concentration-dependent weight average sedimentation coefficient that increased from 1.8 S for the monomer to about 3-6 S for the dimer. Both values agreed with those calculated from the MFE-23 crystal structure. In relation to the thermal stability of MFE-23, denaturation experiments by (1)H NMR and FT-IR spectroscopy showed that the molecule is stable up to 47 degrees C, after which denaturation was irreversible. MFE-23 dimerisation is discussed in terms of a new model for diabody structures, in which the V(H) and V(L) domains in the monomer are able to dissociate and reassociate to form a dimer, or diabody, but in which symmetric back-to-back contacts between the two monomers are formed. This dimerisation in solution is attributed to the complementary nature of the C-terminal surface of the MFE-23 monomer. Crystal structures for

  4. SISTEMA DE ESGOTO DOMÉSTICO

    Directory of Open Access Journals (Sweden)

    Thais Viviane Coimbra

    2017-02-01

    Full Text Available Há muito tempo a população sofre pela carência de programas de saneamento básico,o que acaba causando cada vez mais problemas para a população e para o meio ambiente, pois os efluentes acabam sendo despejados em corpos receptores. Sendo assim, uma solução seria desenvolver alternativas que auxilie no tratamento do esgoto, e que ainda satisfaça as necessidades da população. Portanto, a implantação de um sistema de tratamento de esgoto doméstico seria a alternativa ideal, pois garante um baixo custo de construção e uma excelente eficiência, minimizando assim grande parte dos problemas. Desse modo, com o  dimensionamento de um sistema composto por Fossa Séptica e Sumidouro para uma residência de pequeno porte é possível descrever suas funções e como é realizado o projeto através da norma NBR 7229/1993. Esse tipo de tratamento resultará em uma melhor qualidade dos recursos hídricos  e a qualidade de vida da população.

  5. Viviendas de bajo costo sistema SALVY

    Directory of Open Access Journals (Sweden)

    Suárez Ibarra, Salvador

    1984-06-01

    Full Text Available The aim of this work is to offer an alternative for solving the current problem of housing, through a building system which has been developed and tested in our country — the SAL VY SYSTEM. It is directed towards solving the problem of low-cost housing with own technology, enabling these houses to be built quickly, using materials which are not traditional in building, but are abundant in our country. The house presented by us allows the user to extend its height according to his needs and financial possibilities, keeping the free area of the plot for so-called kitchen gardens.Este trabajo tiene como objetivo presentar una alternativa para la solución del problema actual de la vivienda, a través de un sistema constructivo desarrollado y experimentado en nuestro país —SISTEMA SALVY, dirigido a solucionar el problema de la vivienda de bajo costo, con una tecnología propia que permite la construcción de estas viviendas de una manera rápida, utilizando materiales no tradicionales en construcción, pero abundantes en nuestro país. La vivienda que presentamos permite al usuario ampliarla en altura, de acuerdo a sus necesidades y posibilidades económicas, manteniendo el área libre de la parcela para los denominados huertos del hogar.

  6. Evolución estelar en sistemas binarios

    Science.gov (United States)

    De Vito, M. A.; Benvenuto, O.

    Definición y clasificación de sistemas binarios; descripción del comportamiento del sistema frente a la variación de su masa; binarias de rayos X; transferencia de masa en sistemas binarios masivos aplicado al posible esclarecimiento del progenitor azul de la supernova SN 1987A; comentario acerca de la evolución de enanas blancas de helio de baja masa y su conexión con los sistemas binarios; reseña del trabajo de Kippenhahn y Weigert sobre el cual está basado el código evolutivo desarrollado en la FCAG por el Dr. Benvenuto y sobre el cual se trabajará para poder incluir la evolución de una estrella con pérdida de masa perteneciente a un sistema binario.

  7. Control óptimo y robusto H∞ de sistemas no lineales aplicaciones a sistemas electromecánicos

    OpenAIRE

    Vivas Venegas, Carlos

    2004-01-01

    La teoría de control automático ha experimentado un notable desarrollo en los últimos tiempos especialmente en el campo de los sistemas no lineales. Son aportaciones relativamente recientes los paradigmas de control basados en pasividad, teoría de sistema

  8. Linear concentration system; Sistema de concentracion lineal

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez Lugo, J.I; Leon Rovira, N; Aguayo Tellez, H [Instituto Tecnologico y de Estudios Superiores de Monterrey, Monterrey, Nuevo Leon (Mexico)]. E-mails: a00812662@itesm.mx; noel.leon@itesm.mx; haguayo@itesm.mx

    2013-03-15

    Solar linear concentration technologies to generate high temperatures are limited to the ranges of 200 to 500 degrees Celsius. While its performance has been tested through prototypes and pilot plants around the world, there are still areas of opportunity that can be exploited to obtain a linear concentration that achieves temperatures above this range in order to have a better use of the available solar energy. Because of this: It is possible to develop a linear concentration system that can track the sun with minimal movement of the absorber-receiver while maintaining temperatures above 850 degrees Celsius sufficient for industrial processes that require that temperature. The methodology consists of a series of stages (conceptual design, simulation, evaluation, development concept, results and validation) through which concepts are generated that allow design and evaluation of solar concentrator configurations with the help of simulation software. We have designed a linear parabolic concentrating system which comprises a set of mirrors segments with different focal lengths that works within the range of 600 degrees Celsius; however, it is advancing in the development of a double concentration to reach 850 degrees Celsius. [Spanish] Las tecnologias de concentracion lineal solar para generar altas temperaturas se ven limitadas a los rangos de 200 a 500 grados centigrados. Si bien su funcionamiento ha sido probado a traves de prototipos y plantas piloto alrededor del mundo, aun existen areas de oportunidad que pueden ser aprovechadas para obtener un sistema de concentracion lineal que permita alcanzar temperaturas mayores a este rango para asi tener un mejor aprovechamiento de la energia solar disponible. Debido a esto: Es posible desarrollar un sistema de concentracion lineal capaz de seguir la trayectoria del Sol con minimo movimiento del absorbedor-recibidor al mismo tiempo que mantiene temperaturas superiores a los 850 grados centigrados suficientes para

  9. LA INGENIERÍA DE SISTEMAS Y SU EVOLUCIÓN HACIA LA ARQUITECTURA DE SISTEMAS

    Directory of Open Access Journals (Sweden)

    EDGAR SERNA MONTOYA

    2009-12-01

    Full Text Available Muchas de las empresas modernas entendieron que sus antiguas unidades de sistemas ya no son funcionales, y comienzan a subdividirlas en dos grupos de trabajo diferenciadores: el encargado de la infraestructura y el de los denominados “arquitectos de Sistemas”. Esta decisión lógica la inspira la actual evolución de la Ingeniería de Sistemas que, como área de conocimiento, genera los mismos subgrupos como agentes para formación. Además, la evolución y complejidad de lossistemas de información en medio de la sociedad del conocimiento, con exigencias y expectativas muy complejas, también determinan la necesidad de esta especialización. En este documento, una traducción casi literal de un white paper que publicó la empresa Quidnunc -www.quidnunc.com consultado en abril del año 2000- especializada en gestión de configuración, se detalla la importancia de esta división y las pautas a seguir a la hora de diseñar la arquitectura de sistemas de una empresa

  10. Talento emprendedor, inteligencia, creatividad y sistema educativo

    Directory of Open Access Journals (Sweden)

    Mariano Gutiérrez Tapias

    2014-01-01

    Full Text Available En este artículo, pretendemos realizar un sencillo análisis de términos relacionados con el talento, el emprendimiento y la creatividad por entender que dichos conceptos deben estar presentes en aquellas sociedades que pretenden seguir creciendo en lo cultural, en lo social y en lo económico. Ya decía Einstein que es muy difícil encontrar niños que sigan siendo creativos al terminar la escuela. Este reto debe afrontarlo el sistema educativo si quiere ser de utilidad a la sociedad, favorecer personas críticas y creativas, con pensamientos divergentes, capaces de aprender y desaprender, de reinventarse varias veces a lo largo de su vida.

  11. Defesa dos direitos humanos: sistemas regionais

    Directory of Open Access Journals (Sweden)

    Hélio Bicudo

    2003-04-01

    Full Text Available O texto discorre sobre a institucionalização dos sistemas de promoção dos Direitos Humanos, ao longo do século XX. Tomando a Declaração Universal dos Direitos do Homem, de 1948, como base e ponto de partida, o articulista aborda as três principais vertentes dos sistemas de promoção desses direitos: a européia, a americana e a africana. A evolução de cada uma delas descrita analógica e epistemologicamente, de forma a salientar singularidades sem perder de vista as influências recíprocas ou mesmo as determinantes próprias do contexto espaço-social. Para finalizar, o articulista destaca a importância do Tribunal Penal Internacional no estabelecimento de uma justiça ecumênica. Concomitantemente, alerta para o perigo contido na ameaça de os EUA não o ratificarem.The text underlines the institutional character acquired by the main systems to promote Human Rights in the twentieth century. Taking the Universal Human Rigths Declaration, of 1948, as the basis for analysis, the author brings up three different approaches to promote these rights: the European, the American and the African. The evolution of each of them is described using its procedure as a basic reference. Besides, an analogical analysis, brings to the fore singularities, without ignoring reciprocal influences dictated by particular contexts, though. The author stresses the importance of the International Penal Court, as a means to establish ecumenical justice. At the same time, he reminds the reader of the risks implicit in the possible non-ratification of the IPC by United States.

  12. Sistemas de tiempo real en situaciones de sobrecarga

    OpenAIRE

    Pérez Almansa, Jordi

    2005-01-01

    En el proyecto presentado en esta memoria se estudia el análisis de sistemas de tiempo real en los que se debe verificar el cumplimiento de ciertos requerimientos temporales, principalmente referidos a plazos máximos de ejecución. El análisis de estos requerimientos se basa en el estudio de la Calidad de Control del sistema, es decir, observar como responde el sistema cuando tiene situaciones de sobrecarga. El análisis y diseño en tiempo real se trata de una tarea compleja, que requiere la...

  13. Sistemas integrados de gestión (SIG)

    OpenAIRE

    García Pantigozo, Manuel; Quispe Atúncar, Carlos; Ráez Guevara, Luis

    2014-01-01

    La cultura de la normalización es necesaria para competir globalmente, y para esto la ISO ha elaborado una serie de sistemas de gestión orientados a las calidad, medio ambiente, seguridad en el trabajo y recursos humanos, esto significa la necesidad de integrar sistemas mediante el Sistema integrado de Gestión - SIG The culture of standardization is necessary to compete globally, and to this ISO has developed a number of management systems geared to quality, environment, occupational safe...

  14. Sistemas de reconocimiento basados en la imagen facial

    OpenAIRE

    Arguello Fuentes, Henry

    2012-01-01

    Este artículo sintetiza las principales investigaciones que se están llevando a cabo en el área de los sistemas de reconocimiento a través de la imagen facial. Se realiza la descripción de las principales líneas de trabajo en los sistema de identificación de personas por medio de la imagen del rostro. Además, se realiza una síntesis de las últimas técnicas matemáticas para realizar la extracción de características dentro de estos sistemas de identificación.

  15. MODELADO Y SIMULACIÓN DE SISTEMAS MECATRÓNICOS

    Directory of Open Access Journals (Sweden)

    Mauricio Castillo Effen

    2004-01-01

    Full Text Available En este artículo se introduce el campo de la mecatrónica, su importancia y relación con los sistemas embebidos, procediendo luego a mostrar algunos retos que representa la tarea de modelar y simular sistemas mecatrónicos. Se presentan también algunos paradigmas de modelación y simulación de sistemas de éste tipo así como alternativas prácticas de cómo encarar los retos mencionados.

  16. Vectorization in an oncolytic vaccinia virus of an antibody, a Fab and a scFv against programmed cell death -1 (PD-1) allows their intratumoral delivery and an improved tumor-growth inhibition.

    Science.gov (United States)

    Kleinpeter, Patricia; Fend, Laetitia; Thioudellet, Christine; Geist, Michel; Sfrontato, Nathalie; Koerper, Véronique; Fahrner, Catherine; Schmitt, Doris; Gantzer, Murielle; Remy-Ziller, Christelle; Brandely, Renée; Villeval, Dominique; Rittner, Karola; Silvestre, Nathalie; Erbs, Philippe; Zitvogel, Laurence; Quéméneur, Eric; Préville, Xavier; Marchand, Jean-Baptiste

    2016-01-01

    We report here the successful vectorization of a hamster monoclonal IgG (namely J43) recognizing the murine Programmed cell death-1 (mPD-1) in Western Reserve (WR) oncolytic vaccinia virus. Three forms of mPD-1 binders have been inserted into the virus: whole antibody (mAb), Fragment antigen-binding (Fab) or single-chain variable fragment (scFv). MAb, Fab and scFv were produced and assembled with the expected patterns in supernatants of cells infected by the recombinant viruses. The three purified mPD-1 binders were able to block the binding of mPD-1 ligand to mPD-1 in vitro . Moreover, mAb was detected in tumor and in serum of C57BL/6 mice when the recombinant WR-mAb was injected intratumorally (IT) in B16F10 and MCA 205 tumors. The concentration of circulating mAb detected after IT injection was up to 1,900-fold higher than the level obtained after a subcutaneous (SC) injection (i.e., without tumor) confirming the virus tropism for tumoral cells and/or microenvironment. Moreover, the overall tumoral accumulation of the mAb was higher and lasted longer after IT injection of WR-mAb1, than after IT administration of 10 µg of J43. The IT injection of viruses induced a massive infiltration of immune cells including activated lymphocytes (CD8 + and CD4 + ). Interestingly, in the MCA 205 tumor model, WR-mAb1 and WR-scFv induced a therapeutic control of tumor growth similar to unarmed WR combined to systemically administered J43 and superior to that obtained with an unarmed WR. These results pave the way for next generation of oncolytic vaccinia armed with immunomodulatory therapeutic proteins such as mAbs.

  17. Generation of a rabbit single-chain fragment variable (scFv) antibody for specific detection of Bradyrhizobium sp. DOA9 in both free-living and bacteroid forms.

    Science.gov (United States)

    Vu, Nguyen Xuan; Pruksametanan, Natcha; Srila, Witsanu; Yuttavanichakul, Watcharin; Teamtisong, Kamonluck; Teaumroong, Neung; Boonkerd, Nantakorn; Tittabutr, Panlada; Yamabhai, Montarop

    2017-01-01

    A simple and reliable method for the detection of specific nitrogen-fixing bacteria in both free-living and bacteroid forms is essential for the development and application of biofertilizer. Traditionally, a polyclonal antibody generated from an immunized rabbit was used for detection. However, the disadvantages of using a polyclonal antibody include limited supply and cross-reactivity to related bacterial strains. This is the first report on the application of phage display technology for the generation of a rabbit recombinant monoclonal antibody for specific detection and monitoring of nitrogen-fixing bacteria in both free-living form and in plant nodules. Bradyrhizobium sp. DOA9, a broad host range soil bacteria, originally isolated from the root nodules of Aeschynomene americana in Thailand was used as a model in this study. A recombinant single-chain fragment variable (scFv) antibody library was constructed from the spleen of a rabbit immunized with DOA9. After three rounds of biopanning, one specific phage-displayed scFv antibody, designated bDOA9rb8, was identified. Specific binding of this antibody was confirmed by phage enzyme-linked immunosorbent assay (phage ELISA). The phage antibody could bind specifically to DOA9 in both free-living cells (pure culture) and bacteroids inside plant nodules. In addition to phage ELISA, specific and robust immunofluorescence staining of both free-living and bacteroid forms could also be observed by confocal-immunofluorescence imaging, without cross-reactivity with other tested bradyrhizobial strains. Moreover, specific binding of free scFv to DOA9 was also demonstrated by ELISA. This recombinant antibody can also be used for the study of the molecular mechanism of plant-microbe interactions in the future.

  18. Comparison of the efficiency of antibody selection from semi-synthetic scFv and non-immune Fab phage display libraries against protein targets for rapid development of diagnostic immunoassays.

    Science.gov (United States)

    Chan, Conrad E Z; Chan, Annie H Y; Lim, Angeline P C; Hanson, Brendon J

    2011-10-28

    Rapid development of diagnostic immunoassays against novel emerging or genetically modified pathogens in an emergency situation is dependent on the timely isolation of specific antibodies. Non-immune antibody phage display libraries are an efficient in vitro method for selecting monoclonal antibodies and hence ideal in these circumstances. Such libraries can be constructed from a variety of sources e.g. B cell cDNA or synthetically generated, and use a variety of antibody formats, typically scFv or Fab. However, antibody source and format can impact on the quality of antibodies generated and hence the effectiveness of this methodology for the timely production of antibodies. We have carried out a comparative screening of two antibody libraries, a semi-synthetic scFv library and a human-derived Fab library against the protective antigen toxin component of Bacillus anthracis and the epsilon toxin of Clostridium botulinum. We have shown that while the synthetic library produced a diverse collection of specific scFv-phage, these contained a high frequency of unnatural amber stops and glycosylation sites which limited their conversion to IgG, and also a high number which lost specificity when expressed as IgG. In contrast, these limitations were overcome by the use of a natural human library. Antibodies from both libraries could be used to develop sandwich ELISA assays with similar sensitivity. However, the ease and speed with which full-length IgG could be generated from the human-derived Fab library makes screening this type of library the preferable method for rapid antibody generation for diagnostic assay development. Copyright © 2011 Elsevier B.V. All rights reserved.

  19. Sistemas integrados de gestão empresarial: estudos de casos de implementação de sistemas ERP.

    OpenAIRE

    Cesar Alexandre de Souza

    2000-01-01

    Este trabalho é um estudo das características dos sistemas ERP (Enterprise Resource Planning), de seus processos de escolha, implementação e utilização, de seus benefícios, suas desvantagens e de seus possíveis impactos nas organizações. No levantamento bibliográfico, são apresentados conceitos relacionados aos sistemas ERP, bem como uma proposta de modelo de ciclo para estes sistemas, com a finalidade de estudar suas diferentes etapas na empresa, procurando estabelecer em cada uma delas qua...

  20. Performance of the FV3-powered Next Generation Global Prediction System for Harvey and Irma, and a vision for a "beyond weather timescale" prediction system for long-range hurricane track and intensity predictions

    Science.gov (United States)

    Lin, S. J.; Bender, M.; Harris, L.; Hazelton, A.

    2017-12-01

    The performance of a GFDL developed FV3-based Next Generation Global Prediction System (NGGPS) for Harvey and Irma will be reported. We will report on aspects of track and intensity errors (vs operational models), heavy precipitation (Harvey), rapid intensification, and simulated structure (in comparison with ground based radar), and point to a need of a future long-range (from day-5 up to 30 days) physically based ensemble hurricane prediction system for providing useful information to the forecasters, beyond the usual weather timescale.

  1. Evaluación y Medida de Sistemas de Aprendizaje en Ambientes Colaborativos

    OpenAIRE

    Vega Rodríguez, Pamela M.; Aguilar Carranza, Kathia M.; Paredes Velasco, Maximiliano

    2011-01-01

    La evaluación del sistema de aprendizaje se realiza en base a ciertos criterios y variables que permiten cuantificar, obtener resultados y determinar ventajas y desventajas frente a otro sistema. La evaluación permite verificar que un sistema colaborativo de aprendizaje cumple con los objetivos deseados. En este informe se presenta una revisión de estudios sobre los sistemas de aprendizaje colaborativo y posteriormente una propuesta de evaluación. Lenguajes y Sistemas Inform...

  2. Sistemas adhesivos en Odontología Restauradora

    Directory of Open Access Journals (Sweden)

    María Natalia Mandri

    Full Text Available Los sistemas adhesivos actuales han permitido mejorar los procedimientos clínicos tanto en la evolución de los componentes y su mecanismo de acción, como en la disminución del tiempo operatorio de aplicación de cada uno de ellos, brindando una eficacia clínica aceptable y predecible. Esta demanda de efectividad, ha dado lugar a una gran variedad de sistemas adhesivos, que en muchas ocasiones, no se emplean en la práctica profesional. El propósito de este trabajo es realizar una revisión de los sistemas adhesivos a fin de brindar la información necesaria y secuencia de aplicación, que permita al odontólogo realizar una adecuada selección y utilización del sistema, de acuerdo a la situación clínica

  3. Inmunología tumoral y neoplasias del sistema inmune

    OpenAIRE

    Sen Fernández, María Luz de la; Sempere Ortells, José Miguel; Marco, Francisco M.; Vázquez Araujo, Begoña

    2012-01-01

    Inmunología tumoral: vigilancia inmunológica, antígenos tumorales, respuesta inmune antitumoral, escape tumoral. Inmunología y diagnóstico. Inmunoterapia. Neoplasias del sistema inmune: leucemias y linfomas.

  4. Nuevos sistemas de frecuencia intermedia para el IAR

    Science.gov (United States)

    Olalde, J. C.; Perilli, D.; Larrarte, J. J.

    Se presenta el diagrama en bloques de los nuevos sistemas de Frecuencia Intermedia para los dos radiómetros instalados en el IAR. Entre las características más importantes del sistema podemos mencionar la posibilidad de conectar cualquiera de las dos antenas a los ``backend" disponibles: analizador espectral de alta resolución (META II) de 0,05 Hz, autocorrelador de 1008 canales y contínuo. Se incorporan al sistema nuevos sintetizadores de frecuencia implementados con PLL y la moderna técnica de síntesis digital directa. Por último, el conjunto del sistema es susceptible de ser configurado por las computadoras de adquisición de datos, supervisadas por otra, que entrega el estado de funcionamiento actual y evita la selección de configuraciones incorrectas por parte del usuario.

  5. Envejecimiento del sistema cardiovascular Cardiovascular system aging

    Directory of Open Access Journals (Sweden)

    José M Ocampo

    2005-08-01

    Full Text Available El envejecimiento del sistema cardiovascular está asociado con un número característico de cambios a nivel bioquímico, histológico y morfológico. Sin embargo, no todas las modificaciones presentadas se asocian con deterioro en la función. Entre los cambios a nivel cardiaco se tienen: disminución en el número de miocitos y en las células del sistema de conducción cardiaca, desarrollo de fibrosis, cambios en el transporte de calcio a través de las membranas y disminución del cronotropismo, inotropismo y lusitropismo mediados por estímulo b-adrenérgico. A nivel vascular, hay incremento en la rigidez de la pared de las arterias, con aumento en la velocidad de la onda de pulso, disfunción endotelial y disminución de la vasodilatación mediada por estímulo b-adrenérgico. Durante el reposo el sistema cardiovascular es capaz de desarrollar mecanismos adaptativos eficientes, pero en situaciones de estrés como el ejercicio, los cambios asociados con el envejecimiento se hacen evidentes ya que está disminuida la capacidad para obtener la frecuencia cardiaca máxima, está incrementada la postcarga y hay disminución de la contractilidad intrínseca. Por lo anterior, los ancianos deben utilizar al máximo el mecanismo de Frank-Starling para mantener el gasto cardiaco. Los cambios estructurales y funcionales asociados con el envejecimiento cardiovascular, disminuyen de forma significativa el umbral en el cual las enfermedades cardiacas llegan a ser evidentes, y deben ser conocidos por el personal de salud encargado de cuidar a los ancianos.Cardiovascular aging is associated with characteristic biochemical, histological and morphological changes. Nevertheless, these changes are not necessarily associated to a deterioration in its function. Among the cardiac changes found, there is a reduction in the number of myocytes and of the cardiac conduction system cells, development of fibrosis, changes in the trans-membrane calcium transport and a

  6. Implantación del sistema ERP SAP R/3

    OpenAIRE

    Muñoz Fernandez, Jorge Juan

    2012-01-01

    El objetivo del presente trabajo ha sido realizar un estudio de investigación y desarrollo sobre la implantación del sistema Enterprise Resource Planning (ERP) SAP R/3 de una empresa. L'objectiu d'aquest treball final de carrera ha estat realitzar un estudi d'investigació i desenvolupament sobre la implantació del sistema Enterprise Resource Planning (ERP) SAP R/3 d'una empresa.

  7. Inmunosenescencia: efectos de la edad sobre el sistema inmune

    OpenAIRE

    Saavedra Hernández, Danay; García Verdecia, Beatriz

    2014-01-01

    El proceso de envejecimiento provoca cambios en el sistema inmune que afectan su funcionamiento y desarrollo. Estos cambios pueden manifestarse desde la linfopoyesis hasta la respuesta que orquesta el sistema inmune frente a determinada enfermedad o agente infeccioso. Ambas ramas de la inmunidad, innata y adaptativa, se afectan en este proceso, lo que genera un impacto negativo en la respuesta inmune de los ancianos y los predispone a padecer enfermedades infecciosas, cáncer, autoinmunidad y ...

  8. El riego por chorro intermitente: un sistema prometedor

    Directory of Open Access Journals (Sweden)

    Hernando Buriticá

    1981-07-01

    Full Text Available A través de la historia el hombre se ha ingeniado varios sistemas para suplir las deficiencias de agua que las condiciones naturales de clima han impuesto para el desarrollo de la agricultura: desvió ríos, construyó canales y reservorios y entregó el agua a los cultivos empleando variados sistemas de riego. En este caso haremos referencia al riego por chorro intermitente.

  9. Uma contribuição aos sistemas de custeio

    OpenAIRE

    Neves, Luiz Carlos

    2010-01-01

    Apresenta um modelo de sistema de custeio, que observa as vantagens dos sistemas de custeio direto e custeio por absorção. Aborda as origens da contabilidade e sua evolução até o surgimento da contabilidade de custos. Tece considerações a respeito dos métodos de custeio existentes. Ilustra, com apresentação de exemplos numéricos, o modelo de custeio proposto

  10. Smart: sistemas multi-agente robótico

    Directory of Open Access Journals (Sweden)

    JOVANI ALBERTO JIMÉNEZ BUILES

    2008-01-01

    Full Text Available El siguiente artículo busca dar una visión global de los Sistemas Multi-Agentes Robóticos (MARS mediante una explicación de las áreas relacionadas con el tema para luego presentar el Sistema Multi-Agente Robótico (SMART. SMART es un enjambre inteligente conformado por un robot nodriza y tres robot tipo baliza (guías que navegan de manera colaborativa un escenario estructurado.

  11. Estratégias para luz natural: sistemas inovadores.

    Directory of Open Access Journals (Sweden)

    Juliana Saiter Garrocho

    2007-07-01

    Full Text Available Diversos sistemas, estratégias e componentesinovadores de alta tecnologia paraa otimização do uso da luz natural noprojeto de arquitetura têm se desenvolvidoatualmente, principalmente na EuropaCentral, nos Estados Unidos e na Austrália.Este artigo apresenta algumas dessasestratégias descrevendo suas principaiscaracterísticas. Palavras-chave: luz natural, estratégias deprojeto, sistemas inovadores de alta tecnologia.

  12. Sistemas de serviços de saúde: principais tipologias e suas relações com os sistema de saúde brasileiro

    OpenAIRE

    José Patrício Bispo Júnior; Kelly Leite Maia de Messias

    2005-01-01

    O presente artigo discute as principais características dos Sistemas de Serviços de Saúde e relaciona o Sistema de Saúde Brasileiro com os três principais tipos de Sistemas de Serviços de Saúde. Conceitua e diferencia Sistema de Saúde de Sistemas de Serviços de Saúde. Apresenta a conformação, objetivos, componentes, características e funções dos Sistemas de Serviços de Saúde. Apresenta e discute os três principais tipos de Sistemas de Serviços de Saúde: Sistema de Saúde de Dominância de Me...

  13. Tecnologias e sistemas de tratamento para os dejetos da suinocultura

    Directory of Open Access Journals (Sweden)

    Leandro D. dos Santos

    2015-03-01

    Full Text Available A suinocultura caracteriza-se como uma atividade de alto impacto ambiental, devido a grande quantidade de dejetos com alta carga de poluentes, lançados no meio ambiente. No entanto, existem diversas tecnologias, e sistemas de tratamento, com a capacidade de reduzir o poder poluente dos dejetos da suinocultura. O presente trabalho tem como objetivo analisar as principais tecnologias e sistemas de tratamentos para os dejetos da suinocultura, presentes em publicações brasileiras. Os resultados obtidos nestas publicações apresentaram-se de certa forma satisfatórios em relação à redução da carga de poluentes dos efluentes da suinocultura. Entretanto, pouco se sabe sobre o comportamento da redução dos poluentes em função de parâmetros dimensionais e temporais das tecnologias ou dos sistemas de tratamento. Tal desconhecimento dificulta o dimensionamento de sistemas de tratamento, desconsiderando a relação existente entre investimento e redução do poder poluente dos dejetos. Dentro deste contexto, a modelagem e otimização de sistemas pode ser uma ferramenta muito útil para o dimensionamento de tecnologias e sistemas de tratamento de dejetos de suínos.

  14. Sistemas de vigilancia de riesgos ambientales para la salud. Sistemas de toxicovigilancia

    Directory of Open Access Journals (Sweden)

    Ana Ferrer Dufol

    2004-12-01

    Full Text Available Se presenta el estudio de la Sección de Toxicología Clínica, sobre vigilancia epidemiológica en los Servicios de Urgencias, que tienen como causa intoxicaciones por productos químicos en el periodo de 1999-2003 y dentro del convenio de Asociación Española de Toxicología (AETOX con el Ministerio de Sanidad y Consumo; trabajo presentado en el Congreso Nacional de Medio Ambiente (CONAMA en el Grupo de trabajo “Sistemas de Vigilancia de Riesgos Ambientales para la Salud”.

  15. Viaje alrededor del sistema-mundo

    Directory of Open Access Journals (Sweden)

    Pablo González Casanova

    2015-08-01

    Full Text Available Al iniciar el recorrido, Wallerstein sostiene dos tesis principales: que la historia está abierta, y que vivimos una etapa en que el determinismo disminuye. En un caso enfrenta las tesis de una historia clausurada, en otro, la de un futuro sin alternativas. Ni es el ?n de la historia ni vivimos en el mejor de los mundos posibles. La investigación y la acción tienen que explorar en los terrenos macro y micro de la política, la economía, la cultura y la sociedad y descubrir cómo se abre y cierra la historia del porvenir, y como se abren los límites actuales del sistema-mundo a los valores universales respetando sus particularidades y especi?cidades. El planteamiento de Wallerstein se opone a las tesis de moda de los funcionarios-?lósofos del “establishment” conservador, dizque tecnocientí?co y objetivo. Permite comprender un mundo que está abierto a la libertad aunque no necesariamente la alcance.

  16. Perspectivas de los sistemas de pago

    Directory of Open Access Journals (Sweden)

    Daniel Heymann

    2016-04-01

    Full Text Available ResumenLos agentes, instrumentos y mecanismos que intervienen en la realización de transacciones cotidianas han ido variando considerablemente a lo largo del tiempo, de acuerdo a la evolución de las prácticas, tecnologías e instituciones que determinan costumbres y mecanismos de pago. La organización de los esquemas de intercambio, y de los sistemas financieros en general se ha convertido en tema saliente de discusión contemporánea. Estas notas presentan un breve análisis de la cuestión, centrado sobre los procedimientos para procesar pagos.Código JEL: E42. AbstractThe agents, instruments and mechanisms involved in everyday transactions have varied considerably over time, according to the evolution of practices, technologies and institutions that determine customs and payment mechanisms. The organization of exchange schemes, and financial systems in general, has become salient topic of contemporary discussion. This paper presents a brief analysis of the matter, focused on procedures to process payments. Key words: Monetary Systems,Payment Systems, Currency.Code JEL: E42

  17. Sistema integral para viviendas unifamiliares -SIVIM-

    Directory of Open Access Journals (Sweden)

    Drágula, Josef

    1984-07-01

    Full Text Available The Integral System for Multi-family Housing —SIVIM— is a proposition combining a structural solution and a functional one. In the first, techniques developed in civil construction for reinforced concrete technology are incorporated in the production of buildings. The second one proposes a spatial solution appropriate to the users’ requirements. Thus, with this proposition, the aims of constructing mass, low-cost housing are maintained in countries with tropical climatesEl Sistema Integral para Viviendas Multifamiliares —SIVIM— es una proposición que combina una solución estructural y una solución funcional. En la primera, se incorporan técnicas desarrolladas en la construcción civil, para la tecnología del hormigón armado, a la producción de edificaciones. La segunda, propone una solución espacial apropiada a los requerimientos de los usuarios. Se mantiene así con esta proposición, las metas de la construcción masiva y del bajo costo de la vivienda en países de clima tropical

  18. O sistema social da saúde: conceito, limites e possibilidades

    Directory of Open Access Journals (Sweden)

    Sandra Regina Martini Vial

    2014-06-01

    Full Text Available Discutem-se as possibilidades de definir o sistema da saúde como um sistema social, diferenciado funcionalmente, a partir da ótica da Teoria dos Sistemas Sociais, utilizando os pressupostos gerais da Teoria e a estrutura apresentada por Niklas Luhmann sobre o sistema médico. Apresentam-se alguns “desassossegos” provocados pela Teoria; as possíveis relações entre o sistema da saúde e o que Luhmann chamou de sistema médico e como esse sistema, atualmente, pode ser considerado sistema da saúde; e, por fim, a nossa concepção do sistema da saúde na atualidade.

  19. SUSTENTABILIDADE EM SISTEMAS AGROFLORESTAIS: INDICADORES SOCIOECONÔMICOS

    Directory of Open Access Journals (Sweden)

    Omar Daniel

    2000-03-01

    Full Text Available É ampla a discussão que envolve a importância do enquadramento das atividades de produção em geral, ao conceito de desenvolvimento sustentável. Dentre as atividades agropecuárias, os sistemas agroflorestais (SAF têm sido considerados como sustentáveis, apresentando-se como alternativas aos sistemas intensivos de produção. Para monitorar a sustentabilidade de atividades agropecuárias em geral, incluindo os SAF, diferentes autores enfatizam os indicadores biofísicos, em detrimento dos socioeconômicos. Com o objetivo de definir um rol de indicadores socioeconômicos adaptáveis aos diversos modelos de SAF, desenvolveu-se um estudo consolidado por recomendações de especialistas e ampla revisão de literatura. Concluiu-se que: as categorias relacionadas com a operação dos sistemas comportaram o maior número de indicadores no componente socioeconômico, com maior concentração nas operações endógenas ao sistema, seguidas, de longe, pelos recursos endógenos e exógenos; o maior número de indicadores, sugeridos na categoria operação do sistema, deu-se nos descritores saúde e nutrição, empregos, habitação e saneamento básico e análise econômica; na categoria operação de sistemas exógenos, determinou-se maior número de indicadores para os descritores comercialização e infra-estrutura rural; praticamente, não houve diferença entre o número de indicadores obtidos para os sistemas agroflorestais com e sem o componente animal.

  20. Sistemas híbrido de produção de energia para sistema de comunicações rádio

    OpenAIRE

    Carreiras, José Manuel Patrício

    2014-01-01

    Mestrado em Sistemas de Energias Renováveis na Escola Superior de Tecnologia e Gestão do Instituto Politécnico de Viana do Castelo Este trabalho de projeto incidiu sobre o dimensionamento de cinco sistemas Autónomos de produção de energia para alimentação de Pontos repetidores do sistema de comunicação e sua implementação. O presente trabalho iniciou-se em 2006 quando por motivos profissionais foi necessário construir um sistema de comunicação de suporte ao sistema de telegestã...

  1. Construção de uma biblioteca de fragmentos de anticorpos monoclonais de galinhas com cadeia única (scFv por phage display com reatividade cruzada para estirpes heterólogas do vírus da bronquite infecciosa aviária Construction of an avian single chain monoclonal antibodies (scFv library by phage display that cross-reacted with heterologous avian infectious bronchitis virus strains

    Directory of Open Access Journals (Sweden)

    Camila Cesário Fernandes

    2010-06-01

    Full Text Available Anticorpos monoclonais constituem a base de vários testes usados na detecção e na identificação de antígenos. Nesse contexto, tais imuno-reagentes têm sido extensivamente empregados na identificação de estirpes virais envolvidas na etiologia de surtos de bronquite infecciosa a campo, permitindo o aperfeiçoamento das técnicas de detecção e caracterização antigênica do vírus da bronquite infecciosa das galinhas (VBI. No presente estudo, uma biblioteca de fragmentos de anticorpos de galinha originalmente preparada por phage display contra a estirpe vacinal (H120 do VBI foi usada para a seleção de fragmentos de anticorpos recombinantes com reatividade cruzada para as estirpes heterólogas IBVPR01 e IBVPR05, isoladas de surtos a campo no Brasil e a estirpe SE-17, isolada nos Estados Unidos. Após três ciclos de panning, foi identificado, pelo ELISA, um conjunto de 15 anticorpos scFv expressos em fagos e com reatividade cruzada para essas mesmas estirpes do VBI. A análise por Western-blotting revelou que dois desses clones apresentavam fagos expressando fragmentos de anticorpos monoclonais com reatividade cruzada para a nucleoproteína N das três estirpes do VBI e também para a forma recombinante dessa nucleoproteína derivada da estirpe M41. Concluindo, os fragmentos de anticorpos monoclonais recombinantes scFv-N produzidos em fagos interagem com um epítopo mais conservado da proteína N do VBI e apresentam um grande potencial para utilização na detecção e no diagnóstico direto desse vírus.Monoclonal antibodies are the basis of various techniques used for antigen detection or characterization, and their use is specially recommended for the identification of viral strains involved in the etiology of infectious bronchitis outbreaks. These antibodies are homogeneous, highly specific and fully characterizable, allowing the improvement of immunological techniques detection and antigenic characterization of avian infectious

  2. Sistemas multi–modales de profundidad restringida Multi-modal systems of restricted depth

    OpenAIRE

    Manuel Sierra A.

    2008-01-01

    Se presentan como extensiones del cálculo proposicional clásico, la jerarquíade sistemas deductivos SMM–n con n > 1. SMM–n es el sistema multi–modalde profundidad–n. El sistema SMM–1 es el cálculo proposicional clásico. Elsistema SMM–(n + 1) puede ser visto como el resultado de aplicar la regla denecesariedad, asociada a los razonadores con suficiente capacidad de razona-miento, una vez a los teoremas del sistema SMM–n. El sistema SMM resultade la reunión de los sistemas de la jerarquía, y pu...

  3. En búsqueda del ADN y las influencias en algunos sistemas presidenciales y parlamentarios

    Directory of Open Access Journals (Sweden)

    Jorge Carpizo

    2009-05-01

    Full Text Available El trabajo examina las recíprocas influencias entre las Constituciones de seis países: tres con sistema presidencial, dos con sistema parlamentario y uno con sistema semipresidencial. Los tres últimos pertenecen a Europa. Los sistemas presidenciales de América Latina se inspiraron en la Constitución Americana, tanto como en alguna europea, especialmente la española de 1812. Los enormes poderes presidenciales en América Latina llevaron a introducir en el sistema presidencial controles propios del sistema parlamentario

  4. SISTEMA DE CONTROL INTELIGENTE PARA UN GRUPO DE ELEVADORES

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    Andrés Enrique Rosso Mateus

    2008-01-01

    Full Text Available En este artículo se trata el problema del tráfico vertical en edificios de gran altura y se describe el análisis, diseño y evaluación de un sistema de control para un grupo elevadores que busca mejorar el desempeño respecto a un sistema de control tradicional. El controlador propuesto busca asignar de una forma inteligente las llamadas que se generan a los ascensores que hacen parte del grupo, el desempeño del sistema se traduce en una reducción de los tiempos de servicio y de la energía que invierte el sistema. El controlador propuesto está basado en lógica difusa y las reglas de inferencia expresan el conocimiento de un experto orientado hacia la obtención de los objetivos mencionados. El controlador inteligente se evalúa en un simulador gráfico desarrollado para este fin, este simulador permite ingresar parámetros de configuración para que se adapte y represente una edificación real. Se corrieron dos simulaciones, una usando un controlador convencional y otra usando el controlador inteligente, esto con el fin de comparar su desempeño y cuantificar valores como tiempo de esperar de los usuarios y energía consumida por el sistema.

  5. Gobierno y administración de sistemas de riego

    Directory of Open Access Journals (Sweden)

    Jacinta Palerm-Viqueira

    2005-01-01

    Full Text Available La propuesta es diferenciar gobierno y administración en los sistemas de riego, argumentando que el análisis que permite la caracterización de esta última no se puede realizar observando únicamente el locus de autoridad. Desde la perspectiva de la administración, hay casos de autogobierno en que los regantes mismos realizan todas las tareas con base en sistemas de conocimiento local, otros en que toda la administración se encuentra a cargo de personal contratado. Hay casos de administración burocrática y tecnocrática en sistemas con distinto locus de autoridad (Estado/regantes. Además, se propone que mientras el autogobierno de sistemas de riego no parece tener límites de tamaño, la administración sin burocracia ni persona l especializado parece estar acotada a sistemas de riego de una extensión determinada. No obstante, se argumenta que el gobierno influye sobre el carácter de la administración, dado que su descentralización disminuye o evita que sea burocrática/tecnocrática.

  6. Sistema paraconsistente LBPc¬I Sistema paraconsistente LBPc¬I

    Directory of Open Access Journals (Sweden)

    Manuel Sierra A

    2006-12-01

    Full Text Available El lenguaje del sistema LBPc¬I extiende el lenguaje de la lógica clásica positiva al incluir un operador de negación débil y un operador de incompatibilidad, además, permite definir un operador de negación fuerte; este último tiene todas las características de la negación clásica. El sistema es caracterizado por una semántica de valuaciones tradicionales con la cual se prueba que, respecto al operador de negación débil, el sistema es paraconsistente. Como es de esperarse, cuando las fórmulas involucradas en un argumento se comportan clásicamente, es decir, son incompatibles con su negación débil, entonces la negación débil se comporta como la negación clásica, pero este requisito no siempre es necesario, la negación débil puede ser puntualmente tan potente como la clásica, aunque las fórmulas involucradas no se comporten clásicamente.The language of the LBPc¬I system extends the language of the classical positive logic when including an operator of weak negation and an operator of incompatibility, and permit to define an operator of strong negation; this last one has all the characteristics of the classical negation. The system is characterized by a traditional semantics with which test that, with respect to the operator of weak negation, the system is paraconsistent. When the formulas involved in an argument behave classically, that is to say, are incompatible with his weak negation, then the weak negation behaves like the classical negation, but this requirement not always is necessary, the weak negation can precise be as powerful as the classical negation although the involved formulas do not behave classically.

  7. El sistema de partidos de cataluña

    Directory of Open Access Journals (Sweden)

    Gema Sánchez Medero

    2011-07-01

    Full Text Available : El artículo realiza una radiografía del sistema de partidos políticos de Cataluña, fijando las características sobre el que gira el mismo. Inicialmente presenta un análisis de la evolución del sistema de partidos desde las primeras elecciones autonómicas hasta las últimas celebradas en noviembre de 2010. En una segunda parte estudia el peso que ha tenido cada una de las fuerzas catalanes en cada uno de los comicios que se han celebrado desde 1980 hasta 2010, fijando una serie de variables presentes en el sistema de partidos: predomino del eje centro-derecha y de los partidos nacionalistas, el voto dual y los niveles de abstención.

  8. Sistema de adquisición y procesamiento de audio

    OpenAIRE

    Pérez Segurado, Rubén

    2015-01-01

    El objetivo de este proyecto es el diseño y la implementación de una plataforma para un sistema de procesamiento de audio. El sistema recibirá una señal de audio analógica desde una fuente de audio, permitirá realizar un tratamiento digital de dicha señal y generará una señal procesada que se enviará a unos altavoces externos. Para la realización del sistema de procesamiento se empleará: - Un dispositivo FPGA de Lattice, modelo MachX02-7000-HE, en la cual estarán todas la...

  9. arroz rojo (Oryza sativa L. en dos sistemas de labranza

    Directory of Open Access Journals (Sweden)

    Steven Brenes Prendas

    2006-01-01

    Full Text Available A lo largo de cuatro ciclos de cultivo de arroz anegado, entre los años 2003 y 2004, en la Hacienda El Pelón de la Bajura, Liberia, Guanacaste, Costa Rica, se determinó la distribución espacial de las poblaciones de arroz rojo en los sistemas de Siembra Directa sobre Rastrojos (SDR y en Labranza Mecanizada Convencional (LMC. Mediante levantamientos de malezas ubicados en puntos fijos a nivel de campo y con ayuda del programa Surfer, se logró construir mapas georeferenciales de dichas poblaciones. El sistema SDR logró disminuir el ritmo de crecimiento de las poblaciones, facilitando su manejo, por lo contrario el sistema LMC, favoreció: la distribución, el aumento y la densidad de las poblaciones de arroz rojo en el campo. Los mapas de georeferencia facilitarían la toma de decisiones en el manejo integrado del arroz rojo

  10. Calagem na superfície em sistema plantio direto

    OpenAIRE

    E. F. Caires; D. A. Banzatto; A. F. Fonseca

    2000-01-01

    Existem informações conflitantes a respeito da eficiência da aplicação superficial de calcário em sistema plantio direto, particularmente na correção da acidez do subsolo, e de critérios de recomendação de calagem para tal sistema de cultivo. Com o objetivo de avaliar a correção da acidez do solo e a produção de grãos de culturas em rotação no sistema plantio direto, considerando a aplicação de calcário na superfície, foi realizado um experimento em um Latossolo Vermelho-Escuro distrófico tex...

  11. Interface: Sistema orientado à visualização

    Directory of Open Access Journals (Sweden)

    Alexandre Braga

    2015-06-01

    Full Text Available A criação de ambientes interativos para multiplataformas implica necessariamente o design de interface. Este artigo está dedicado às questões relativas à interface humano-computador cujas funções são definidas como sistema. Este apresenta atributos específicos que, neste caso, são tratados no contexto dos sistemas humano-máquina. Para que ambos os elementos possam interagir, é preciso haver um objetivo, ou seja, uma tarefa comum. Em função disso, as interfaces de usuário são observadas pela perspectiva do sistema humano-tarefa-computador, cujas interações são fundamentais para a compreensão da interface computacional.  

  12. Métodos de control en sistemas domóticos: últimas tendencias en sistemas distribuidos

    Directory of Open Access Journals (Sweden)

    Moreno Navarro, I.

    1999-02-01

    Full Text Available Control systems lie amongst the most difficult issues to be tackled when it comes to designing home automation systems. Two classical tendencies may be considered to solve this problem. The first one consists of a centralised control system, whereas the second option advocates for a distributed network, where piece would take up certain responsibilities in the control of the whole system. In this paper we analyse some of the latest developments as regards programming distributed networks, focusing on home automation, specially Java and the latest Jini environments have dramatically improved the potential of such kind of distributed systems, thanks to them, it is possible to build a heterogeneous network including devices from different brands which work in collaborative systems, thus avoiding complex configurations.

    Uno de los aspectos más delicados a la hora de diseñar un sistema domótico es el que se refiere al modelo de control que deseamos establecer. Podemos considerar dos tendencias clásicas: la que trata de establecer un control centralizado del sistema y la que propone un control mucho más descentralizado, donde cada aparato seria responsable de cierta parte de la gestión del control ejercido sobre toda la red. En este articulo tratamos de profundizar en las últimas novedades aparecidas en el campo de la programación de los controladores asociados a una red domótica, en particular Java y el más reciente Jini, que aportan una serie de soluciones que facilitan la compatibilidad entre dispositivos de distintos fabricantes y apuestan claramente por una arquitectura de tipo distribuido, dotando a las redes domóticas de una flexibilidad y un potencial de crecimiento inusitados.

  13. Control lineal robusto de sistemas no lineales diferencialmente planos

    Directory of Open Access Journals (Sweden)

    Hebertt Sira-Ramirez

    2011-01-01

    Full Text Available Resumen: En este trabajo se proponen controladores basados en observadores lineales para el control robusto de una clase amplia de sistemas no lineales conocidos como sistemas diferencialmente planos, sean estos monovariables o multivariables. Se establece primeramente el modelo dinámico entrada - salida plana, simplificándolo a un modelo de carácter no fenomenológico que solamente considera como elementos claves en el diseño del controlador el orden de integración del sistema y el factor de ganancia de entrada, en el caso monovariable, y, los órdenes de los subsistemas de integración de Kronecker y la matriz de ganancias del vector de entradas en el caso multivariable. El resto de las no linealidades, dependientes del estado o de naturaleza exógena, son consideradas, en general, como perturbaciones desconocidas pero acotadas que toman valores en el tiempo. Se demuestra que estas perturbaciones son algebraicamente observables, permitiendo su determinación aproximada mediante observadores lineales de orden arbitrario. Estos observadores, llamados observadores GPI, incluyen modelos internos que representan polinomios en el tiempo, cuya actualización es de índole automática, permitiendo aproximaciones arbitrariamente cercanas a las perturbaciones desconocidas. El diseño del controlador se reduce entonces a lograr la cancelación de las perturbaciones aditivas a la vez de imponer una dinámica lineal en lazo cerrado mediante realimentación de estados estimados, los cuales se obtienen directamente del mismo observador lineal propuesto. Se presenta un ejemplo de simulación que considera un sistema físico no lineal de complejidad reconocida. También se incluyen resultados experimentales sobre dos prototipos de laboratorio. Palabras Clave: Rechazo a perturbaciones, Realimentación lineal de salida, Sistemas linealizables, Observadores, Sistemas no lineales

  14. Calidad en el desarrollo de Sistemas de Software

    OpenAIRE

    Pesado, Patricia Mabel; Bertone, Rodolfo Alfredo; Esponda, Silvia; Pasini, Ariel C.; Martorelli, Sabrina Lorena

    2010-01-01

    El objetivo del subproyecto “Calidad en el desarrollo de Sistemas de Software" es analizar, investigar y desarrollar propuestas en temas relacionados con el aseguramiento de la calidad en cada una de las etapas del desarrollo de Sistemas de Software. En particular se ha trabajado y estudiado las normas de calidad más ampliamente difundidas, líneas ISO y CMMI, y su incidencia en entornos como la pequeña y mediana industria de nuestro país. Desde el año 2006, se ha trabajado en la adecuación...

  15. Evaluación de un sistema experimental de acuaponia

    OpenAIRE

    M. García-Ulloa; C. León; F. Hernández; R. Chavéz

    2005-01-01

    Se evaluó un sistema experimental de acuaponia, incorporando la producción de tilapia (Oreochromis mossambicus) y pepino (Cucumis sativus) durante 75 días. Los peces (90 g de peso promedio inicial) se mantuvieron en un tanque de 500 l, a una densidad de 0.6 peces/litro, mientras que 40 plántulas de pepino se sembraron en 2 camas de arena estéril. Las plantas se regaron con agua de desecho de las tilapias, con un sistema de recirculación de agua. Cada semana se registraron las concentraciones ...

  16. Sistema instrumentado para o registro de comportamentos ingestivos

    OpenAIRE

    Pereira, Gustavo Vieira

    2003-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia Elétrica. Este trabalho descreve um sistema automático para registro de parâmetros que permitam o estudo de atributos do comportamento ingestivo de animais em laboratório. Este sistema é orientado a usuários que não são profissionais da área de engenharia. Portanto, dispensa a necessidade de configurações eletrônicas que são desestimulantes para biólogos, fisiologist...

  17. Implementación del sistema para municipios, usando pmi

    OpenAIRE

    Cruz Ruiz, Victor Hugo; Buenaño Vera, Sulay Pamela; Freire Cobo, Lenin

    2014-01-01

    El objetivo de informe de graduación, es plantear y describir la implementación de un sistema informático en una entidad municipal usando PMI, siguiendo las pautas definidas en la Guía del PMBOK. Nuestro estudio es aplicar la Metodología Administración de Proyectos PMI a un proyecto ya terminado. Para este proyecto hemos tomado la información de la implementación del Sistema CABILDO ERP en el Gobierno Municipal de San Jacinto de Yaguachi, realizado por la empresa ecuatoriana...

  18. Funciones del Fiscal en el Sistema Procesal Penal Acusatorio Ecuatoriano

    OpenAIRE

    Guillén, Oscar Medardo

    2006-01-01

    El art.219 de la Constitución Política vigente, define el nuevo marco legal, para el ejercicio de la acción penal de instancia pública, del Ministerio Público, en concordancia con el Nuevo Código de Procedimiento Penal y la Ley Orgánica del Ministerio Público, instrumentos jurídicos que han permitido el cambio del sistema conocido como inquisitivo , caracterizado por la concentración de funciones en el juez, para dar paso al sistema acusatorio oral que se ha dicho es mas humano, democrático y...

  19. Linfoma do sistema nervoso central: ensaio iconográfico

    Directory of Open Access Journals (Sweden)

    Fabiano Reis

    2013-04-01

    Full Text Available Ilustramos este ensaio iconográfico de linfoma do sistema nervoso central com imagens de ressonância magnética obtidas em nosso serviço nos últimos 13 anos e discutimos algumas das principais características radiológicas deste tipo de linfoma, primário e secundário. O linfoma sistema nervoso central é um tumor relativamente infrequente, mas alguns achados na ressonância magnética podem sugerir este diagnóstico.

  20. Sistema genérico de replicación

    OpenAIRE

    Jiménez Ortiz, Raúl

    2014-01-01

    Este proyecto se desarrolla en una de las principales compañías eléctricas de España, y trata sobre la creación de un sistema genérico de replicación de datos. La idea surge de la necesidad de estandarizar la forma de traspasar información entre departamentos o sistemas, debido a que existen gran variedad de interfaces diferentes, y cada vez que surge la necesidad de crear una implica nuevos costes de desarrollo, mantenimiento y futuros evolutivos. Entre los diferentes departamentos de ...

  1. Sistemas de Inteligencia Web basados en redes sociales

    OpenAIRE

    Rosa Troyano, Fco. Fernando de la; Martínez Gasca, Rafael

    2007-01-01

    El Análisis de las Redes Sociales (ARS) es un área que está emergiendo como imprescindible en los procesos de toma de decisiones. Su capacidad para analizar e intervenir una red social puede ser aprovechada para implantar tareas de vigilancia en los sistemas de inteligencia de un centro de investigación o una empresa de base tecnológica. El objetivo de este trabajo es realizar una propuesta para diseñar sistemas de inteligencia web basados en redes sociales. El primer obstáculo para implantar...

  2. Sistemas de Inteligencia Web basados en Redes Sociales

    OpenAIRE

    de la Rosa Troyano, Fco. Fernando; Martínez Gasca, Rafael

    2007-01-01

    El Análisis de las Redes Sociales (ARS) es un área que está emergiendo como imprescindible en los procesos de toma de decisiones. Su capacidad para analizar e intervenir una red social puede ser aprovechada para implantar tareas de vigilancia en los sistemas de inteligencia de un centro de investigación o una empresa de base tecnológica. El objetivo de este trabajo es realizar una propuesta para diseñar sistemas de inteligencia web basados en redes sociales. El primer obstáculo para implantar...

  3. Sistemas Correctores de Campo Para EL Telescopio Cassegrain IAC80

    Science.gov (United States)

    Galan, M. J.; Cobos, F. J.

    1987-05-01

    El proyecto de instrumentación de mayor importancia que ha tenido el Instituto de Astrofisica de Canarias en los últimos afios ha sido el diseflo y construcción del te1escopio IAC8O. Este requería del esfuerzo con junto en mec´nica, óptica y electrónica, lo que facilitó la estructuración y el crecimiento de los respectivos grupos de trabajo, que posteriormente se integraron en departamentos En su origen (1977), el telescopio IAC80 fue concebido como un sistema clásico tipo Cassegrain, con una razón focal F/i 1.3 para el sistema Casse grain y una razón focal F/20 para el sistema Coudé. Posteriormente, aunque se mantuvo la filosofia de que el sistema básico fuera el F/11.3, se consideró conveniente el diseño de secundarios para razones focales F/16 y F/32, y se eliminó el de F/20. Sin embargo, dada la importancia relativa que un foco estrictamente fotográfico tiene en un telescopio moderno, diseñado básicamente para fotometría fotoeléctrica y con un campo util mínimamente de 40 minutos de arco, se decídió Ilevar a cabo el diseño de un secundario F/8 con un sistema corrector de campo, pero que estuviera formado únicamente por lentes con superficies esféricas para que asl su construcción fuera posible en España ó en México. La creciente utilización de detectores bidimensionales para fines de investigación astron6mica y la viabilidad de que en un futuro cercano éstos tengan un área sensible cada vez mayor, hicieron atractiva la idea de tener diseñado un sistema corrector de campo para el foco primario (F/3), con un campo útil mínimo de un grado, y también con la limitante de que sus componentes tuvieron sólamente supérficies esféricas. Ambos diseños de los sis-temas correctores de campo se llevaron a cabo, en gran medida, como parte de un proyecto de colaboración e intercambio en el área de diseño y evaluación de sistemas ópticos.

  4. Sistemas adhesivos en Odontología Restauradora

    OpenAIRE

    Mandri, María Natalia; Aguirre Grabre de Prieto, Alicia; Zamudio, María Eugenia

    2015-01-01

    Los sistemas adhesivos actuales han permitido mejorar los procedimientos clínicos tanto en la evolución de los componentes y su mecanismo de acción, como en la disminución del tiempo operatorio de aplicación de cada uno de ellos, brindando una eficacia clínica aceptable y predecible. Esta demanda de efectividad, ha dado lugar a una gran variedad de sistemas adhesivos, que en muchas ocasiones, no se emplean en la práctica profesional. El propósito de este trabajo es realizar una revisión de lo...

  5. El sistema visual y el sueño

    OpenAIRE

    Garrido Lozano, Ana Isabel

    2015-01-01

    A pesar de la gran cantidad de tiempo que invertimos durmiendo, el sueño sigue siendo, hoy en día, un gran desconocido. Sin embargo, sabemos que es un estado -indispensable para la vida- activo del sistema nervioso mediante registro electroencefalográfico. Para que tenga lugar el inicio del sueño, el núcleo supraquiasmático debe recibir señales del sistema visual, el cual indica la disminución de luz. Cuando inicia el proceso del sueño, éste pasa por diferentes fases caracterizadas por la no ...

  6. Debido proceso, sistemas y reforma del proceso penal

    Directory of Open Access Journals (Sweden)

    Teresa Armenta Deu

    2015-03-01

    Full Text Available Este artículo se propone examinar los modelos de procesos penales de los movimientos de reforma que se establecieron en las últimas décadas del siglo XX, en los países iberoamericanos. Para ello, primero se enfatiza las características históricas de cada sistema, poniendo de relieve la importancia de no confundir el acusatorio con el adversarial, rechazando la coincidencia entre los sistemas actuales y mixto inquisitivo histórico. Apoya la importancia de la igualdad de armas y e del contradictoria en la búsqueda de un equilibrio entre los modelos de procedimiento.

  7. Diseño de Sistema de Inventario Corporativo

    OpenAIRE

    Solis Chusan, Harry Eduardo

    2015-01-01

    La Tesis sobre “Diseño de Sistema de Inventario Corporativo” nos va a indicar como solo un sistema de inventario podrá facilitar el manejo de la empresa en este ámbito, siendo este modificable en el transcurso del tiempo para cumplir con las necesidades que se requieran. Prestando ayuda en el control y distribución en los insumos que proveen, nos dará un indicativo si necesita más productos en stock o se necesitan ponerlos en descuento por el tiempo que ha tenido gua...

  8. Diseño de Sistema de Inventario Coorporativo

    OpenAIRE

    Yanten Gomez, Yaan Carlos

    2015-01-01

    La Tesis sobre “Diseño de Sistema de Inventario Corporativo” nos va a indicar como solo un sistema de inventario podrá facilitar el manejo de la empresa en este ámbito, siendo este modificable en el transcurso del tiempo para cumplir con las necesidades que se requieran. Prestando ayuda en el control y distribución en los insumos que proveen, nos dará un indicativo si necesita más productos en stock o se necesitan ponerlos en descuento por el tiempo que ha tenido guardado, todo esto para evit...

  9. Sistema de detección de malware en Android

    OpenAIRE

    Heras Cáceres, Inés; Sierra Liras, Diego

    2015-01-01

    El presente trabajo trata de dar respuesta al problema de la detección del malware en los dispositivos móviles. Los sistemas de protección que actualmente poseen los dispositivos móviles inteligentes se han mostrado ineficaces. Para asegurarlos frente a una posible infección de software malicioso, debe completarse la seguridad que ofrecen las características del sistema o la criba de aplicaciones en los mercados de descarga oficiales. Siendo un área de investigación reciente, la mayor parte d...

  10. TECNOLOGÍAS ASOCIADAS AL SISTEMA POWER TO GAS

    OpenAIRE

    Perez Gil, Susana; Belsue Etxeberria, Mikel

    2017-01-01

    El sistema "Power-to-Gas" (PtG) permite almacenar energía eléctrica mediante su transformación en energía química en forma de hidrógeno o metano. Estos gases pueden ser inyectados a la red de gas natural para su posterior distribución y uso. En este artículo se explican las diferentes tecnologías de las que consta el sistema PtG: la electrolisis, para la producción de hidrógeno, y la metanación, para la producción de metano.

  11. Algoritmo de cifrado para sistemas móviles

    OpenAIRE

    Castro Lechtaler, Antonio; Cipriano, Marcelo; García, Edith; Liporace, Julio César; Maiorano, Ariel; Malvacio, Eduardo; Tapia, Néstor

    2017-01-01

    Este proyecto de investigación persigue elaborar el diseño y desarrollo de un Algoritmo de Cifrado para Sistemas Móviles que por sus propiedades de velocidad, compactibili- dad y robustez; pueda ser implementado en equipos de comunicaciones que funcionan so- bre Sistemas Móviles. Se esperan obtener resultados teóricos, prácticos y la realización de un desarrollo experimental. Los modernos algoritmos criptológicos responden a principios y filosofías diferentes a las que se llevaban a cab...

  12. Sistemas de software distribuidos y bases de datos distribuidas

    OpenAIRE

    Pesado, Patricia Mabel; Ramón, Hugo Dionisio; Thomas, Pablo Javier; Boracchia, Marcos; Champredonde, Raúl; Pasini, Ariel C.; Chichizola, Franco; Iglesias, Marina; Marrero, Luciano; Albanesi, María Bernarda; Delía, Lisandro Nahuel; Ricci, Guillermo

    2006-01-01

    El proyecto de investigación y desarrollo en Sistemas de Software Distribuido, abarca aspectos que van desde los fundamentos del desarrollo (Técnicas de Ingeniería de Requerimientos, Metodologías de Gestión y Desarrollo de Proyectos, Técnicas de Planificación, Métricas, Normas de Calidad, Web-Systems) hasta la concepción de aplicaciones específicas orientadas a los procesos industriales (Sistemas de Planeamiento de Producción, Control Industrial en tiempo real), procesos de E-government (Voto...

  13. Calidad en el desarrollo de Sistemas de Software

    OpenAIRE

    Pesado, Patricia Mabel; Bertone, Rodolfo Alfredo; Ramón, Hugo Dionisio; Pasini, Ariel C.; Esponda, Silvia; Alonso, Laura Mabel

    2006-01-01

    El proyecto de investigación y desarrollo “Sistemas de Software Distribuidos. Aplicaciones en procesos industriales, E-government y E-learning” abarca aspectos que van desde los fundamentos del desarrollo (Técnicas de Ingeniería de requerimientos, Metodologías de Gestión y Desarrollo de Proyectos, Técnicas de Planificación, Métricas, Normas de Calidad, Web-Systems) hasta la concepción de aplicaciones específicas orientadas a los procesos industriales (sistemas de planeamiento industrial, cont...

  14. Sposobnost opstanka informacionih sistema / Survivability of information systems

    Directory of Open Access Journals (Sweden)

    Boško Rodić

    2005-03-01

    Full Text Available U radu je opisan značaj i trend razvoja informacionih sistema u savremenom društvu, a definisani su i drugi značajni pojmovi radi boljeg razumevanja problema. Prikazana su i četiri aspekta rešenja sposobnosti opstanka informacionih sistema koje predlaže Computer Emergency Response Team (CERT. / The article deals with importance and trend of information systems in modern society. In the article are defined, beside survivability, other relevant ideas necessary for better understanding of the problems. Also, in the article are illustrated four aspects of information system survivability solution by Computer Emergency Response Team (CERT.

  15. Modelado y análisis de sistemas fotovoltaicos

    OpenAIRE

    Guasch Murillo, Daniel

    2003-01-01

    Bajo la etiqueta de "Modelado y análisis de sistemas fotovoltaicos" se presentan los trabajos realizados a lo largo del doctorado. Estos estudios centran su aplicación en el área de los sistemas fotovoltaicos. Tienen por objetivo conseguir un entorno de trabajo que permita analizar la problemática asociada a instalaciones fotovoltaicas desde su diseño hasta su puesta en marcha y posterior explotación. Se ha escogido Matlab/Simulink como núcleo del entorno de trabajo debido a la gran potencia ...

  16. La guerra de los sistemas operativos V1.0

    OpenAIRE

    López Get, Anthony

    2010-01-01

    Este artículo analiza tres tipos diferentes de sistemas operativos para computadoras —Windows®, Mac OS® y las distribuciones GNU/Linux®— como objetos culturales, desde la ideología, el mito y la cultura material. Se estudia como estos programas de ordenador se erigen como símbolos representativos de diferentes grupos culturales, al reproducir hábitos de consumo, diferenciación social, y discursos hegemónicos, sub y contraculturales, todos dentro del ámbito de los sistemas de producción actual...

  17. Sistemas de controle gerencial e contratos psicológicos

    OpenAIRE

    Ivan Canan

    2013-01-01

    Esta pesquisa investiga a relação entre os sistemas de controle gerencial e os contratos psicológicos, como forma de discutir as relações contratuais nas organizações. Os sistemas de controle gerencial foram definidos como processos administrativos de proposição de parâmetros comportamentais idealmente construídos para criar convergência de objetivos entre servidores e organizações, cuja dinâmica de controle implica no diagnóstico de resultados e nos processos de interação acerca do desempenh...

  18. Desarrollo histórico del sistema sanitario de Ecuador

    OpenAIRE

    Castillo Morocho, Silvia

    2015-01-01

    En este trabajo se describen las condiciones de salud de Ecuador y con mayor detalle, las características del sistema ecuatoriano de salud, incluyendo su estructura y cobertura, programas de salud, fuentes de financiamiento, los recursos físicos, materiales y humanos de los que dispone, las tareas de rectoría que desarrolla el Ministerio de Salud Pública. La República de Ecuador no escapó de los efectos de las sucesivas crisis económicas, y su sistema de salud estuvo marcado por más de 15 ...

  19. Desarrollo e implementación del sistema experto Diagnogsys

    OpenAIRE

    Halcón Fernández, Alejandro

    2015-01-01

    El presente proyecto consiste en el diseño y la implementación de un sistema experto multidisciplinar y polivalente capaz de resolver problemas que se le plantearían a un experto humano de una determinada materia o disciplina. Este documento explicará y detallará el diseño y el funcionamiento del sistema experto Diagnogsys aplicado al ámbito de los diagnósticos médicos, cuyo principal objetivo consistirá en simular a un experto humano en medicina general, como ejemplo del funcionamiento de...

  20. Sistema de enseñanza/aprendizaje inteligente para grafos

    OpenAIRE

    Martínez, Natalia; Ferreira, Gheisa; García, Zoila Zenaida

    2007-01-01

    La teoría de grafos es un tema de estudio de la Matemática Discreta que por su importancia está presente en asignaturas de la carrera Ciencia de la Computación. Dificultades presentadas con respecto a su aprendizaje, han conducido al diseño e implementación de un Sistema de Enseñanza/Aprendizaje Inteligente (SEAI) que aborde esta temática. Los SEAI se caracterizan por aplicar técnicas de Inteligencia Artificial (IA) al desarrollo de sistemas de enseñanza asistida por computadoras, donde el té...

  1. Una Visión General del Sistema Financiero Colombiano

    OpenAIRE

    Hernando Zuleta G.

    1997-01-01

    Desde finales de los 80 el sistema financiero colombiano ha experimentado cambios sensibles. En efecto, la liberalización financiera, el fortalecimiento de la regulación prudencial, la conversión de un número importante de sociedades en establecimientos de crédito, el aumento en los requisitos de capital, etc. han determinado un cambio de perfil en el sistema. Adicionalmente, en el pasado reciente las autoridades han tomado medidas en cuanto a la estructura de los encajes, aumentos en los req...

  2. Functional characterization of two scFv-Fc antibodies from an HIV controller selected on soluble HIV-1 Env complexes: a neutralizing V3- and a trimer-specific gp41 antibody.

    Directory of Open Access Journals (Sweden)

    Maria Trott

    Full Text Available HIV neutralizing antibodies (nAbs represent an important tool in view of prophylactic and therapeutic applications for HIV-1 infection. Patients chronically infected by HIV-1 represent a valuable source for nAbs. HIV controllers, including long-term non-progressors (LTNP and elite controllers (EC, represent an interesting subgroup in this regard, as here nAbs can develop over time in a rather healthy immune system and in the absence of any therapeutic selection pressure. In this study, we characterized two particular antibodies that were selected as scFv antibody fragments from a phage immune library generated from an LTNP with HIV neutralizing antibodies in his plasma. The phage library was screened on recombinant soluble gp140 envelope (Env proteins. Sequencing the selected peptide inserts revealed two major classes of antibody sequences. Binding analysis of the corresponding scFv-Fc derivatives to various trimeric and monomeric Env constructs as well as to peptide arrays showed that one class, represented by monoclonal antibody (mAb A2, specifically recognizes an epitope localized in the pocket binding domain of the C heptad repeat (CHR in the ectodomain of gp41, but only in the trimeric context. Thus, this antibody represents an interesting tool for trimer identification. MAb A7, representing the second class, binds to structural elements of the third variable loop V3 and neutralizes tier 1 and tier 2 HIV-1 isolates of different subtypes with matching critical amino acids in the linear epitope sequence. In conclusion, HIV controllers are a valuable source for the selection of functionally interesting antibodies that can be selected on soluble gp140 proteins with properties from the native envelope spike.

  3. Alternativas eficientes para procesamiento y comunicaciones en sistemas de tiempo real

    OpenAIRE

    Romero, Fernando; Méndez, Mariano; Encinas, Diego; De Giusti, Armando Eduardo; Tinetti, Fernando Gustavo; Medina, Santiago; Pi Puig, Martín; Paniego, Juan Manuel; Dell'Oso, Matías

    2017-01-01

    El objetivo de esta línea de investigación es el estudio y desarrollo de Sistemas de Tiempo Real (STR), en particular, la planificación de tareas y la comunicación en Sistemas Distribuidos de Tiempo Real (SDTR), experimentando con microcontroladores y simulaciones para la adquisición y control de las variables del sistema.

  4. El Sistema Fundamentals in Practice: An Examination of One Public Elementary School Partnership in the US

    Science.gov (United States)

    Simpson Steele, Jamie

    2017-01-01

    El Sistema is a Venezuelan program of social change that has inspired a worldwide movement in music education. El Sistema inspires social transformation and musical excellence to occur simultaneously and symbiotically. This study examines: What does El Sistema look like within the context of a public school partnership in the United States? How do…

  5. Linfoma no-Hodgkin del sistema nervioso central

    OpenAIRE

    Iglesias Rozas, José Rafael, 1942-

    2000-01-01

    Diecisiete imágenes de un linfoma no-Hodgkin del sistema nervioso central en una paciente de 66 años. Seventeen pictures of a non-Hodgkin lymphoma of the central nervous system in a 66-year-old female patient.

  6. Fertilidad del suelo a largo plazo en sistemas biointensivos

    NARCIS (Netherlands)

    Funes Monzote, F.R.; Hernándes, A.; Bello, R.; Alvarez, A.

    2008-01-01

    Mejorar y mantener la fertilidad de los suelos son prioridades para los sistemas agroecológicos. Junto a la preservación de la agrodiversidad, el uso eficiente del agua, la energía y otros recursos disponibles, un adecuado balance de nutrientes y vida en el suelo son condiciones importantes para

  7. Seguridad en Sistemas de Información

    OpenAIRE

    Valverde Álvarez, Evangelino

    2012-01-01

    Material de apoyo de la charla "Seguridad en Sistemas de Información (un recorrido a vista de pájaro)" para los alumnos de primer curso de la Escuela Superior de Informática de Ciudad Real (Abril de 2012).

  8. PERSPECTIVAS DO SISTEMA DE TRANSPORTE URBANO DE LONDRINA - PARANÁ

    Directory of Open Access Journals (Sweden)

    Mirian Vizintim Fernandes Barros

    2014-03-01

    Full Text Available O artigo propõe indicar os problemas encontrados no atual sistema de transporte urbano na cidade de Londrina - Paraná, marcado pela predominância do uso do transporte individual e do coletivo ineficiente e de alto custo. Para tanto, utilizou-se ferramenta dos Sistemas de Informação Geográfica (SIG para, por meio da manipulação dos dados, confirmar a ideia da intrínseca e necessária integração entre o planejamento do uso e ocupação do solo (sobretudo da densidade demográfica e do transporte urbano, essencial para delinear diretrizes de melhorias no sistema coletivo de deslocamento. Identificado os principais problemas, propostas são traçadas e, algumas perspectivas são discutidas objetivando, além da melhoria no sistema de transporte urbano de Londrina, a reflexão sobre o ideal de uma cidade inclusiva, do ponto de vista dos transportes.

  9. Sistemas transparentes para gobiernos electrónicos eficientes

    Directory of Open Access Journals (Sweden)

    Andrade Castro, Jesús Alberto

    2007-05-01

    Full Text Available Este trabajo hace una reflexión crítica del papel que desempeñan los sistemas transparentes en el gobiernoelectrónico. La misión de los sistemas transparentes computarizados es desarrollar aplicaciones confiables y robustas,con el propósito de sustituir la fiscalización y los controles jurídicos y contables del comportamiento administrativo,por verdaderas evaluaciones que incluyan la participación del ciudadano, en el ejercicio transparente de la accióngubernamental. Teniendo como base la necesidad de tener aplicaciones para gobierno electrónico, el Laboratorio deInvestigación de Tecnologías y Sistemas de Información (LITSI de la Facultad de Ciencias de la Universidad del Zuliadesarrolla aplicaciones de minería de texto, para obtener datos que están envueltos en el metalenguaje de etiquetas(HTML contenido en las páginas WEB. Con el prototipo que hemos desarrollado, se ha hecho un intento por convertirinformación desde documentos tipos texto no estructurados que están en la WEB, en información factible de ser analizaday contrastada con las acciones y políticas públicas. Se pretende así, desarrollar sistemas transparentes eficientescon aplicaciones computarizadas que permitan al ciudadano ejercer el control social de la gestión gubernamental.

  10. The Story of Carora: The Origins of El Sistema

    Science.gov (United States)

    Carlson, Alexandra

    2016-01-01

    Venezuela's youth symphony program, the Fundación Musical Simón Bolívar, commonly referred to as "El Sistema," combines musical achievement with learning important life skills through orchestral practice and performance. Although the history most commonly reported outside Venezuela is of the program's director, José Antonio Abreu,…

  11. Al CERN prima fase sistema gestione dati LHC

    CERN Multimedia

    2003-01-01

    "Al via la prima fase per la realizzazione del sistema Lhc computing Grid (LCG), progettato per elaborare le quantita' di dati senza precedenti che, a partire dal 2007, saranno prodotte dagli esperimenti eseguiti con il nuovo grande acceleratore Large Hadron Collider (LHC), presso il Cern di Ginevra" 1 page

  12. Sistema de agua entubada de Cambugán

    OpenAIRE

    Mayorga, O.H.

    2004-01-01

    Los usos principales del agua de Cambugán son lavar ropa y actividades domésticas. Quienes acceden a otro sistema de mejor calidad usan el agua para beber. Hay problemas de sedimentos, mal sabor y olor. Da recomendaciones para educación y otros estudios específicos.

  13. A TEORIA DE SISTEMAS APLICADA À SAÚDE

    Directory of Open Access Journals (Sweden)

    Maria Coeli Campedelli

    Full Text Available A autora tece considerações sobre a Teoria de Sistemas e sua aplicação na saúde e faz uma análise sucinta de alguns aspectos desta Teoria que foram abordados na V Conferência Nacional de Saúde, 1975.

  14. Sistema endocanabinoide: modificando los factores de riesgo cardiovascular

    Directory of Open Access Journals (Sweden)

    John Edwin Feliciano Alfonso

    2006-10-01

    Full Text Available La necesidad de alcanzar un tratamiento óptimo para el tabaquismo, la obesidad y sus comorbilidades, conocidos factores de riesgo cardiovascular, ha fomentado la búsqueda de objetivos terapéuticos novedosos. Es el caso del sistema endocanabinoide, involucrado en diversos fenómenos fisiológicos entre los que se encuentran el refuerzo de ciertos comportamientos y la regulación del apetito. La sobreactivación de este sistema altera la homeostasis corporal predisponiendo a dependencias o a un aumento en la ingesta alimentaria, lo que puede traducirse en tabaquismo u obesidad. La intervención farmacológica sobre el sistema endocanabinoide puede contribuir al manejo de estos factores de riesgo cardiovascular, teniendo en cuenta que a tales beneficios se suman otros independientes de la suspensión del tabaquismo o la reducción de peso, como el aumento del colesterol de alta densidad, la disminución de triglicéridos y la mejoría del control glucémico en pacientes con diabetes. Ensayos clínicos controlados aleatorizados adelantados en poblaciones con diferentes características, han evaluado la utilidad de la regulación farmacológica del sistema endocanabinoide; confirmando su eficacia en personas con factores de riesgo cardiovascular establecidos.

  15. Proyecto SAFEBUS: Sistemas Avanzados de Seguridad Integral en Autobuses

    Directory of Open Access Journals (Sweden)

    Leopoldo Armesto

    2016-01-01

    Full Text Available Resumen: Es bien conocido a partir de estudios previamente realizados, la elevada accidentabilidad de los autobuses urbanos en situaciones de baja velocidad que hacen más necesarios sistemas de asistencia a la conducción. En este sentido, el artículo describe los resultados obtenidos como consecuencia del desarrollo del proyecto “Sistemas Avanzados de Seguridad Integral en Autobuses” (SAFEBUS. Concretamente, se centra en los aspectos de seguridad exterior del autobús, proponiendo un sistema para las situaciones de baja velocidad, paradas o arrancadas del autobús. En él se describen las ideas conceptuales del proyecto y los subsistemas que lo conforman: sistema de detección de personas y sistema avanzado de asistencia al conductor, incluyendo el diseño de dispositivos de realimentación háptica y audio-visual. Así mismo, se detallan los experimentos y validaciones realizados tras la implantación del mismo en autobuses de la compañía Castrosua S.A., los cuales demuestran la capacidad de alertar al conductor y en caso necesario detenerlo en situaciones de peligro. Abstract: It is well known from previous studies, that accidents of buses in urban scenarios, with low speed profiles, make necessary to introduce driving assistance systems. In this sense, this paper describes the results of the SAFEBUS research project “Sistemas Avanzados de Seguridad Integral en Autobuses”. It focuses in safety aspects proposing a system valid at low speed profiles, basically at bus stops. Here, we describe the main ideas exploited in the project and their results, that is, a people detection system together with a driving assistance system with audiovisual and haptic feedback. We also show some experiments and validations carried out at the facilities of Castrosua S.A. company. Keywords: Advance driving assistance systems, people detection, bus safety, Palabras clave: Sistemas de Seguridad Avanzados

  16. O Direito à Saúde no Sistema Prisional

    Directory of Open Access Journals (Sweden)

    Gabrielle Jacobi KOLLING

    2013-04-01

    Full Text Available O direito à saúde é objeto de constante análise e, a cada momento, visualiza-se a necessidade de enfrentar diferentes interfaces desse direito fundamental. A complexidade inerente ao sistema social, e, por conseguinte, ao da saúde também, demanda-nos romper com a classificação de cidadãos de primeira e de segunda classe; isso não é republicano, tampouco democrático. A lógica dos desumanizados e excluídos (esquecidos e negligenciados deve ser cambiada pela lógica da inclusão. A análise enfocará a saúde no sistema prisional. Para isso, é condição de possibilidade analisarmos os condicionantes de saúde e os seus efeitos no panorama prisional-sanitário brasileiro. Observaremos, ainda, as ações governamentais para efetivar o direito à saúde no sistema prisional, em especial a Política Pública de Saúde “Plano Nacional de Saúde no Sistema Penitenciário”, que se apresenta como uma tentativa concreta de romper com a negligência do Estado em relação aos integrantes do sistema prisional, aos esquecidos, aos sem voz. ABSTRACT The right to health is matter of constant analysis and, at each moment the need to challenge the different interfaces of this basic right is visible. The innate complexity of the social system and, by consequence to the health system as well, demands that we break with the concept of first and second class citizens; this is not republican or even democratic. The de-humanized and excluded logic (forgotten and neglected must be traded for the inclusion logic. The analysis will focus the prison system’s health. For this is condition of possibility to analyze the health conditions and its effects in the Brazilian prison-sanitary scope. We will also observe the government’s actions to make the right to heath effective in the prison system, in special the Public Health Policy “Nacional Health Plan in the Prison System”, which is presented as a real attempt to break with the state’s negligence

  17. Sistemas Integrales de Gestión para Bibliotecas

    Directory of Open Access Journals (Sweden)

    Estefania Gomez Vega

    2016-04-01

    Full Text Available Los Sistemas Integrales de Gestión para Bibliotecas (SIGB, constituyen una herramienta fundamental en el dominio de la educación superior para la óptima gerencia del patrimonio bibliográfico. En este trabajo, se busca determinar cuál de los 2 (dos SIGB candidatos es el más adecuado para heredar las funciones de gestión de las Bibliotecas Académicas de la UNPA. Los SIGB a comparar son los sistemas Koha (reconocido como el primer software de gestión de bibliotecas libre, y PMB (también un software libre que está cobrando gran reconocimiento en numerosos países europeos. Este estudio comparativo se origina básicamente por dos motivos: (i el cambio en la reglamentación vigente producido en 2011 y, como consecuencia, la necesidad de que el sistema utilizado por las Bibliotecas de la UNPA cumpla con esta reglamentación, y (ii la necesidad de aumentar la compatibilidad del sistema instalado para que pueda ser accedido y utilizado en su totalidad desde dispositivos portátiles, tales como celulares y tablets. A tal efecto, el presente trabajo propone un Framework Comparativo para analizar los SIGB candidatos aplicando un conjunto de Criterios de Evaluación, tanto a nivel de sistema, como así también, a nivel de módulo. Los resultados de esta investigación tienen como objetivo asistir y proveer sustento a la selección del SIGB que sea más adecuado a las necesidades de la UNPA.

  18. Arquitectura para sistemas de memoria organizacional en instituciones de educación superior

    OpenAIRE

    Quilca Condori, Alexander Edwin

    2014-01-01

    El presente trabajo de investigación tiene como objetivo definir una arquitectura para sistemas de Memoria Organizacional en Instituciones de Educación Superior. El objetivo surge del problema identificado, en el cual la construcción de sistemas de gestión del conocimiento es tratada como construcción de sistemas típicos, aplicando métodos iterativos que si bien proporcionan versiones tempranas del sistema, no permiten predecir y gestionar adecuadamente el desarrollo del sistema. Además, d...

  19. Sistema Político Internacional e Globalização: Continuidade ou Rotura?

    OpenAIRE

    Abreu Varanda, Artur Jorge

    2016-01-01

    O presente trabalho realizado no âmbito da unidade currucular - Teoria da relações internacionais - 4º Ano procura identificar os efeitos das Globalização no Sistema Político Internacional. Para tal, é primeiro definido o conceito de Sistema Político Internacional, partindo do conceito de Sistema para chegar à definição de Kenneth Waltz, inserida no Realismo Estruesturalista, sendo ainda explorada a complexidade do sistema. Em seguida, é caracterizada a evolução do Sistema Político Internacio...

  20. En búsqueda del ADN y las influencias en algunos sistemas presidenciales y parlamentarios

    OpenAIRE

    Jorge Carpizo

    2009-01-01

    El trabajo examina las recíprocas influencias entre las Constituciones de seis países: tres con sistema presidencial, dos con sistema parlamentario y uno con sistema semipresidencial. Los tres últimos pertenecen a Europa. Los sistemas presidenciales de América Latina se inspiraron en la Constitución Americana, tanto como en alguna europea, especialmente la española de 1812. Los enormes poderes presidenciales en América Latina llevaron a introducir en el sistema presidencial controles propios ...

  1. En búsqueda del ADN y las influencias en algunos sistemas presidenciales y parlamentarios

    OpenAIRE

    Carpizo, Jorge

    2009-01-01

    El trabajo examina las recíprocas influencias entre las Constituciones de seis países: tres con sistema presidencial, dos con sistema parlamentario y uno con sistema semipresidencial. Los tres últimos pertenecen a Europa. Los sistemas presidenciales de América Latina se inspiraron en la Constitución Americana, tanto como en alguna europea, especialmente la española de 1812. Los enormes poderes presidenciales en América Latina llevaron a introducir en el sistema preside...

  2. Complejidad y sistemas complejos Complexidade e sistemas complexos Complexity and complex systems

    Directory of Open Access Journals (Sweden)

    Mario Tarride

    1995-06-01

    Full Text Available O pensamento sistêmico e a complexidade revestem-se de especial interesse para a compreensão dos mais diferentes tipos de fenômenos. A necessidade de usar complementarmente análise e síntese, a avaliação da realidade de maneira holística, onde a convivência com a complexidade é um fato que se aceita e não se nega através do reducionismo, assim como o surgimento da ordem a partir do caos e a participação ativa do 'modelizador de sistemas' no desenho de artefatos a partir da linguagem, são alguns dos materiais que compõem este particular esforço de síntese do pensamento de determinados autores.Systemic thinking and complexity are of special interest when it comes to understanding a wide spectrum of phenomena. The present paper synthesizes some authors' ideas regarding a number of topics in this area: the need to use analysis and synthesis complementarity; the holistic evaluation of reality, where living with complexity is an accepted fact, not negated through reductionism; the emergence of order from chaos; and the active participation of a 'model-creator' in the design of artifacts from language.

  3. PRESENTACIÓN DE LA CARRERA DE INGENIERÍA DE SISTEMAS

    Directory of Open Access Journals (Sweden)

    EDGAR SERNA MONTOYA

    2009-06-01

    Full Text Available En el año 2009 la Facultad cuenta con los siguientes programas activos:* Pregrado en Ingeniería de Sistemas Presencial Registro Calificado Código SNIES 11846 Resolución No. 2293 del 03 de octubre de 2002. Título que otorga: Doble titulación, Ingeniero de Sistemas y Tecnólogo en Sistemas.* Ciclo de Profesionalización para Tecnólogos en Sistemas Presencial Título que otorga: Ingeniero de Sistemas.* Tecnología en Sistemas de Información Presencial Registro Calificado Código SNIES 11850 Título que otorga: Tecnólogo en Sistemas de Información.* Especialización en Gerencia de la Tecnología Presencial Registro Calificado Código SNIES 53168 Título que otorga: Especialista en gerencia de la Tecnología.

  4. Selección e implantación de un sistema ERP de código abierto

    OpenAIRE

    Fuertes García, Sonia

    2013-01-01

    En este Trabajo Fin de Carrera estudiaremos el proceso de implantación de un sistema de planificación de recursos empresariales (sistema ERP) de código abierto en una empresa previamente definida. El proceso abarca tanto la selección entre los diversos sistemas ERP de código abierto disponibles actualmente en el mercado, como la implantación del sistema. El sistema ERP elegido es un sistema en la nube. En aquest Treball Fi de Carrera estudiarem el procés d'implantació d'un sistema de plani...

  5. LA REFORMA DEL SISTEMA GENERAL DE SEGURIDAD SOCIAL EN SALUD

    Directory of Open Access Journals (Sweden)

    Gustavo Malagón Londoño

    2011-01-01

    Full Text Available

    La Academia Nacional de Medicina de Colombia en repetidas ocasiones, desde el año 1998, ha denunciado las notables defi ciencias del Sistema General de Seguridad Social en Salud (SGSSS que implantó la Ley 100 de diciembre 23 de 1993. 

    El SGSSS, que debiera ser sistema de aseguramiento con propósito social, se ha convertido en negocio donde priman los intereses económicos con descuido en la calidad de los servicios; hizo profunda crisis, principalmente por causa de los excesivos costos que conlleva el modelo de intermediación comercial. Se ha creado una inaceptable posición oligopólica dominante de los aseguradores, que parece haber suplantado al estado en la defi nición de las políticas y en la orientación del SGSSS e interfiere la buena prestación de los servicios. Manifestación prominente de esto es la creciente y descontrolada integración vertical. 

    El sistema colombiano de intermediación comercial es costoso, inequitativo, ineficiente y va contra los principios constitucionales que definen la salud como derecho humano, en detrimento del bien común.

    El SGSSS, a los 18 años de su implantación, aún no cuenta con un sistema de información y las bases de datos existentes en las EPS, y también en el FOSYGA, distan de ser confiables. Se reconoce que un sistema de salud es en esencia un sistema de información. Los hospitales y clínicas de Colombia, especialmente las instituciones de carácter público, tienen una cartera que, se estima, es cinco o seis veces mayor que la cartera reclamada por las EPS, buena parte de la cual ya es de difícil cobro. Sin embargo, la prioridad ha sido resolver el problema económico de las EPS, no el de los hospitales.

    Son muchas las voces que claman por un riguroso control de la manera como las EPS invierten los recursos de la salud, de carácter público por ser recursos parafiscales. Las EPS muestran un grande y rápido crecimiento patrimonial y han

  6. Evaluación de un sistema experimental de acuaponia

    Directory of Open Access Journals (Sweden)

    M. García-Ulloa

    2005-01-01

    Full Text Available Se evaluó un sistema experimental de acuaponia, incorporando la producción de tilapia (Oreochromis mossambicus y pepino (Cucumis sativus durante 75 días. Los peces (90 g de peso promedio inicial se mantuvieron en un tanque de 500 l, a una densidad de 0.6 peces/litro, mientras que 40 plántulas de pepino se sembraron en 2 camas de arena estéril. Las plantas se regaron con agua de desecho de las tilapias, con un sistema de recirculación de agua. Cada semana se registraron las concentraciones de amonia no ionizada, nitritios y nitratos. Al final del cultivo, los peces crecieron 25 g, en promedio, y se produjeron casi 5 kg de pepino. Las curvas de compuestos nitrogenados mostraron un flujo de nutrientes para las plantas y aporte de agua sin niveles peligrosos de amonia y nitritos para los peces.

  7. Sistema inteligente para evaluación de programas tutores

    Directory of Open Access Journals (Sweden)

    Luz Daicy Alvarado

    2002-01-01

    Full Text Available El mecanismo evaluador que se presenta a continuación forma parte de un proyecto que busca el desarrollo de un Sistema de enseñanza asistida por ordenador (EAO basado en tutores inteligentes. Se pretende que este mecanismo, basado en herramientas de «aprendizaje automático», tenga la capacidad para capturar una «imagen» que indique el grado de comprensión de la materia por parte del alumno e identifique los aspectos en los que el estudiante tiene dificultades. Con esa información se retroalimentará a un sistema tutor de manera que este último tome una decisión acerca de la estrategia educativa por seguir.

  8. VPLIV ELEKTRIČNIH VOZIL NA ZANESLJIVOST ELEKTROENERGETSKEGA SISTEMA

    OpenAIRE

    BOŽIČ, DUŠAN

    2017-01-01

    Zagotavljanje zanesljive oskrbe odjemalcev z električno energijo predstavlja primarno nalogo elektroenergetskih sistemov, na kar kaže tudi spreminjanje načina regulacije sistemskih operaterjev v dereguliranih trgih z električno energijo. Regulatorji pri regulaciji sistemskih operaterjev namreč vse pogosteje uvajajo regulacijo kakovosti dobave električne energije, ki v ospredje postavlja prav zanesljivost sistema. Prihodki sistemskih operaterjev so pri regulaciji kakovosti dobave električne en...

  9. TEORÍA DE SISTEMAS EN LAS ORGANIZACIONES

    OpenAIRE

    Alejandro Hernández Lugo

    2003-01-01

    Reflejar y caracterizar dos puntos de vista con los que se pueden enfocar y administrar el trabajo en una empresa, y conocer las ventajas y desventajas que tiene una visión respecto a la otra puede ser un buen comienzo para aplicar la teoría de sistemas en las organizaciones.

  10. Enfermedades poco comunes: una prioridad de los sistemas de salud

    OpenAIRE

    RAP

    2017-01-01

    Las enfermedades poco comunes, incluidas las de origen genético, son aquellas enfermedades que tienen una prevalencia baja, menor de 5 casos por cada 10.000 habitantes en la comunidad, según la definición de la Unión Europea. Estas enfermedades tienen ciertas características comunes. Son, en su mayor parte, degenerativas y constituyen una prioridad de los sistemas de salud.

  11. LA DIVERSIDAD DE ESPECIES ÚTILES Y SISTEMAS AGROFORESTALES.

    Directory of Open Access Journals (Sweden)

    Hermilio Navarro Garza

    2012-01-01

    Full Text Available El manejo y uso de biodiversidad sustentan servicios agroecosistémicos multifuncionales: aprovisionamiento de alimentos, forrajes, leña, madera, combustibles, fibra y recarga de acuíferos, entre otros. La pérdida de diversidad biológica y los riesgos crecientes de su deterioro, inherentes frecuentemente a diversas actividades humanas, plantean la necesidad de desarrollar capacidades institucionales y sociales para su restauración y conservación. El objetivo de este trabajo fue identificar y describir la diversidad de árboles y arbustos, sus usos y tipos de sistemas agroforestales. Lo importante es conocer la multiplicidad de usos, como expresión sintética de lógicas funcionales que viabilizan los sistemas agroforestales territoriales. La metodología fue descriptiva, basada en talleres participativos orientados a conocer la diversidad de recursos y usos. Posteriormente, selección de unidades familiares, transectos, entrevistas, colecta y clasificación de ejemplares. Se identificaron 81 especies y 34 familias. La diversidad se amplifica por la multifuncionalidad de usos para 55.6 % de especies: 7.42 %, con cinco tipos de usos; 11.1 % con cuatro tipos; 14.8 %, con tres; y 22.2 % con dos. Tipos de usos: leña, 41 especies; medicinales, 30; elaboración de utensilios, 29; madera, 25; fines alimenticios, 23; forrajeras, 20, y cercos vivos, seis especies. De los sistemas familiares, 91.7 % utilizan sistemas agrosilvícolas, y 41.7 %, silvopastoriles, según diversos propósitos socioeconómicos y combinaciones de usos.

  12. Intervencion psicoeducativa en adolescentes con tumores del sistema nerviosos central

    OpenAIRE

    Grau Rubio, Claudia; Hernández Núñez-Polo, Mercedes

    2002-01-01

    Se describen los resultados de la evaluación neuropsicológica y de un programa de intervención psicoeducativa y apoyo emocional aplicados a 5 adolescentes afectados por un tumor del sistema nervioso central. Este programa se desarrolló en ASION (Asociación de padres de niños enfermos de cáncer de la Comunidad de Madrid).

  13. Bifurcaciones del Sistema de FitzHugh-Nagumo (FHN

    Directory of Open Access Journals (Sweden)

    Fernando Ongay Larios

    2011-01-01

    Full Text Available La familia paramétrica de los sistemas de FitzHugh-Nagumo es rica en bifurcaciones (Rocsoreanu et al., 2000. En este artículo estudiamos las bifurcaciones silla-nodo y de Hopf desde el punto de vista matemático de esta familia y se describen completamente los conjuntos de bifurcación en el espacio de parámetros.

  14. Prefabricación en madera. El sistema H B

    OpenAIRE

    Editorial, Equipo

    2017-01-01

    Los elementos HB constituyen una nueva forma de empleo de la madera en la construcción de pabellones industriales. El elemento HB fundamental se compone de una sección "I", en la cual tanto el alma como las alas están formadas por secciones normales de madera, cuyas fuerzas internas se transmiten por un sistema combinado de encolado y clavado.

  15. El Sistema de Referencia Celeste convencional de la IAU

    Science.gov (United States)

    Arias, E. F.

    La Unión Astronómica Internacional (IAU) recomendó la adopción de un nuevo sistema de referencia celeste baricéntrico cuyo plano fundamental y origen de ascensiones rectas estén próximos, respectivamente, al ecuador y equinoccio dinámicos J2000.0. El nuevo sistema de referencia estará materializado por las posiciones J2000.0 de radiofuentes extragalácticas determinadas con la técnica de interferometría de larga línea de base (VLBI). El Working Group on Reference Frames de la IAU (WGRF) decidió adoptar (Grasse, 1995) al sistema de referencia celeste extragaláctico del Servicio Internacional de la Rotación Terrestre (IERS) como futuro sistema de referencia celeste convencional bajo el nombre International Celestial Reference System (ICRS) y encomendó su mantenimiento futuro al IERS. El marco de referencia que materializará al ICRS contiene posiciones precisas J2000.0 de más de 600 radiofuentes extragalácticas. Las coordenadas fueron ajustadas en una única solución VLBI en la cual se incluyeron todas las observaciones realizadas hasta octubre de 1995 con la técnica de adquisición de datos VLBI Mark III. Para minimizar los errores sistemáticos que pueden afectar la calidad del marco de referencia se introdujeron mejoras sustanciales en la modelización y en la selección de datos. Un subconjunto de objetos del marco de referencia se utilizó para referir las posiciones estelares determinadas con el satélite astrométrico Hipparcos al ICRS.

  16. Modelo de Comportamiento Afectivo para Sistemas Tutores Inteligentes

    OpenAIRE

    Hernández Pérez, María Y.

    2008-01-01

    Las emociones se han reconocido como parte fundamental de la motivación, y la motivación como un componente indispensable en el aprendizaje. En este documento se propone un modelo de comportamiento afectivo para sistemas tutores inteligentes. Dicho modelo combina el estado afectivo y pedagógico de los estudiantes para establecer las acciones tutoriales. En el contexto de este trabajo, el comportamiento afectivo tiene dos funciones principales: 1) inferir el estado afectivo del estudiante; y 2...

  17. Intelligent tutorial system for selftraining in tuning of control systems; Sistema tutorial inteligente para el autoentrenamiento en sintonizacion de sistemas de control

    Energy Technology Data Exchange (ETDEWEB)

    Romero Jimenez, Guillermo; Perez Ocampo, Maria Concepcion [Instituto de Investigaciones Electricas, Temixco, Morelos (Mexico)

    1999-07-01

    In this paper the design, development and validation of an intelligent tutorial system oriented to the instruction of techniques of tuning of control systems is described. This system is based on systems previously developed in the Simulation Unit of the Instituto de Investigaciones Electricas (IIE). The designed system accounts with four modules: of knowledge, the student model, of tutor and of interface, basic characteristics that allows to locate this system in the context of the intelligent tutorial systems. In this system in particular, the knowledge module was only modified, because advantage is taken of the existing structure to incorporate a new dominion of application: the one of the techniques of tuning of control systems. The system maintains the characteristic that it can also be used as a consultation system. In addition to the design and validation of the tutorial system, when following the methodology of processing the degree of generality of the developed system, was evaluated, taking into account the evaluation and quantification of metrics that the engineering software proposes. [Spanish] En este trabajo se describen el diseno, el desarrollo y la validacion de un sistema tutorial inteligente orientado a la instruccion de tecnicas de sintonizacion de sistemas de control. Este sistema esta basado en sistemas desarrollados anteriormente en la Unidad de Simulacion del Instituto de Investigaciones Electricas (IIE). El sistema disenado cuenta con cuatro modulos: de conocimiento, del modelo del estudiante, de tutor y de interfaz, caracteristica principal que permite ubicar a este sistema en el contexto de los sistemas tutoriales inteligentes. En este sistema en particular solo se modifica el modulo de conocimiento, pues se aprovecha la estructura existente para incorporar un nuevo dominio de aplicacion: el de las tecnicas de sintonizacion de sistemas de control. El sistema mantiene la caracteristica de que tambien puede utilizarse como un sistema de

  18. El sistema inmunológico de las mucosas

    Directory of Open Access Journals (Sweden)

    Miriam Zaldívar Ochoa

    2002-10-01

    Full Text Available El sistema inmunológico es muy importante porque protege al organismo de agentes microbianos patógenos, toxinas, partículas extrañas, células tumorales y procesos autoinmunes. Para mantener el equilibrio biológico se necesita que este sistema funcione normalmente, de manera que constituya una fuerte barrera defensiva contra la invasión de agentes nocivos; de no suceder esto, se presentará una inmunodeficiencia de expresión clínica variable. La esencia de la función del sistema inmune es su gran capacidad para la discriminación a escala molecular entre lo propio y lo ajeno, o sea, lo que pertenece al organismo y lo que no, conservando así su individualidad.The immunological system is very important because it protects the organism from pathogenic microbial agents, toxins, foreign particles, tumoral cells and autoimmune processes. To maintain the biological balance, this system should work normally, acting as a strong defensive barrier against the invasion of harmful agents. Otherwise, there will be an immunodeficiency of variable clinical expression. The essence of the function of the immune system is its great capacity for distinguishing its own from the strange at the molecular level, that is, to recognize a difference between what belongs or not to the organism, keeping this way its individuality.

  19. Radioisotope measurement system; Sistema de medicion de radioisotopos

    Energy Technology Data Exchange (ETDEWEB)

    Villanueva Ruibal, Jose [Comision Nacional de Energia Atomica, Ezeiza (Argentina).Centro Atomico Ezeiza

    2007-07-01

    A radioisotope measurement system installed at L.M.R. (Ezeiza Atomic Center of CNEA) allows the measurement of nuclear activity from a wide range of radioisotopes. It permits to characterize a broad range of radioisotopes at several activity levels. The measurement hardware as well as the driving software have been developed and constructed at the Dept. of Instrumentation and Control. The work outlines the system's conformation and its operating concept, describes design characteristics, construction and the error treatment, comments assay results and supplies use advices. Measuring tests carried out employing different radionuclides confirmed the system performing satisfactorily and with friendly operation. (author) [Spanish] Un sistema de instrumentacion instalado en el L.M.R. (Centro Atomico Ezeiza de la CNEA) mide en amplio rango la actividad del decaimiento nuclear de radioisotopos. Permite caracterizar una amplia gama de radioistopos de variados niveles de actividad. Tanto el hardware de medicion como el software de operacion han sido desarrollados y construidos en el Dept. Instrumentacion y Control. El trabajo esboza la conformacion del sistema y su concepto de operacion, describe caracteristicas de disenio, construccion y del tratamiento del error, comenta resultados de ensayos y provee recomendaciones de uso. Pruebas de medicion realizadas empleando diversos nucleidos comprobaron que el sistema funciona en forma satisfactoria y su operacion es amigable. (autor)

  20. SISTEMA DE ASEGURAMIENTO DE LA CALIDAD MEDIANTE LA APLICACIÓN DEL SISTEMA HACCP EN LA INDUSTRIA DE PASTAS ALIMENTICIAS

    Directory of Open Access Journals (Sweden)

    Aleida González González

    2005-09-01

    Full Text Available

    En este trabajo se presenta un estudio del HACCP (Hazard Analysis Critical Control Points como un sistema de aseguramiento de la calidad en lo referente a la inocuidad para la industria de alimentos, mencionando las técnicas generalmente usadas para llevar a acabo este estudio y mostrando un ejemplo mediante su aplicación en la industria de pastas alimenticias. Como resultado se elaboró la tabla de control HACCP, la misma que recopila los PCC, el peligro posible, los límites críticos, las medidas correctivas, sistema de monitoreo y responsables de las acciones tomadas.

  1. El sistema fiduciario en el Perú: propuesta para la adopción de un sistema fiduciario mixto

    OpenAIRE

    Vivanco Luyo, Fernando Jesús

    2017-01-01

    El fideicomiso es una antigua institución jurídica cuyo nacimiento se atribuye al derecho romano, con una gran influencia del trust anglosajón. Para el Perú resulta ser una institución con poco más de 23 años de aplicación desde su incorporación a nuestra legislación, allá por el año 1993. Actualmente contamos con los “fideicomisos bancarios”, denominados así por encontrarse regulados en la Ley General del Sistema Financiero, del Sistema de Seguros y Orgánica de la Superintende...

  2. HD 208905: um sistema múltiplo de estrelas quentes

    Science.gov (United States)

    Candeias, J. P.; Daflon, S.; Cunha, K.

    2003-08-01

    Durante o survey de associações OB do disco Galáctico, foi constatada a multiplicidade do sistema HD 208905, pertencentes à associação de Cep OB2. Este objeto está classificado como uma estrela pertencente a um sistema múltiplo, com magnitude mv = 7.0 e tipo espectral B1V. De fato, os espectros de HD 208905 apresentam perfis de absorção triplicados. Dois dos perfis são bastante similares entre si, e são estreitos e bem definidos, sugerindo que as velocidades rotacionais projetadas (v sin i) das duas estrelas são baixas. Os espectros obtidos também apresentam perfis mais alargados que poderiam ser atribuídos a uma terceira componente estelar com v sin i mais alto. A análise de HD 208905 é baseada no estudo da variação da posição relativa dos perfis espectrais de acordo com a fase do sistema. Nossos dados observacionais são um conjunto de espectros de alta resolução obtidos no McDonald Observatory (Universidade do Texas, Austin), Kitt Peak National Observatory e Palomar Observatory, cobrindo o período de 10/91 até 12/95. Inicialmente, calculamos a velocidade radial de cada componente do sistema, considerando o desvio Doppler sofrido por cada estrela. As velocidades radiais medidas foram, em seguida, corrigidas para velocidades radiais heliocêntricas. O passo seguinte constituiu na determinação da periodicidade da série temporal definida pelas medidas das velocidades radiais heliocêntricas através da análise de Fourier. A nossa base de dados não permitiu definir uma solução única para o sistema HD 208905. As possíveis soluções encontradas têm períodos entre 1 e 27 dias e serão apresentadas e discutidas.

  3. Interacciones radiculares en sistemas agroforestales: mecanismos y opciones de manejo

    Directory of Open Access Journals (Sweden)

    F. Casanova

    2007-01-01

    Full Text Available Los sistemas agroforestales (SAF son una forma de uso de la tierra en donde las leñosas interactúan con los cultivos y/o animales, con la finalidad de diversificar y optimizar la producción de manera sostenida. Sin embargo, estos sistemas tienen limitantes originados por una combinación inadecuada de las diferentes especies, lo que resulta en competencia entre ellas. Las características radiculares de las especies leñosas tienen un papel importante en el éxito de los SAF, ya que indican modificaciones que pudieran existir entre especies asociadas. Por tanto, el objetivo de esta revisión es analizar la importancia de las interacciones radiculares, los criterios para el manejo con base en las características morfológicas y patrones de crecimiento entre especies y su impacto en los SAF. La incorporación de especies arbóreas dentro de los cultivos requiere de objetivos claros; es decir, conocer el papel que desempeñarían dentro del sistema. No es redituable utilizar especies arbóreas que no poseen cierto valor o producto, ya que la competencia entre los árboles y los cultivos es solamente admisible si es compensada a través de porlas ventajas con relación a los aumentos en la productividad de sistema. Son deseables aquellas especies arbóreas cuyas raíces sean agresivas con relación a los sistemas radiculares del cultivo asociado, y que manifiesten un crecimiento lateral profundo y/o posean una alta plasticidad. Una opción para reducir la competencia radicular es la regulación del espaciamiento y/o distribución de las diversas especies asociadas, así como la aplicación de prácticas de manejo. Los patrones de la actividad radicular de las plantas difieren entre especies, su conocimiento puede ayudar a evitar competencia excesiva y pérdidas de nutrientes en SAF con la asignación óptima del espacio y recursos disponibles.

  4. La evolución de sistemas complejos: el caso del sistema inmune en animales

    Directory of Open Access Journals (Sweden)

    Luis Fernando Cadavid Gutierrez

    2009-10-01

    Full Text Available Resumen   El sistema inmune en animales comprende una serie de mecanismos celulares y moleculares que de manera conjunta mantienen la integridad fisiológica y genética de los organismos. Convencionalmente se ha considerado la existencia de dos clases de inmunidad, la innata y la adaptativa. La primera es ancestral, con variabilidad limitada y baja discriminación, mientras que la segunda es altamente variable, específica y restringida a vertebrados mandibulados. La inmunidad adaptativa se basa en receptores de antígeno que se rearreglan somáticamente para generar una diversidad casi ilimitada de moléculas. Este mecanismo de recombinación somática muy probablemente emergió como consecuencia de un evento de transferencia horizontal de transposones y transposas bacterianas en el ancestro de los vertebrados mandibulados. El reciente descubrimiento en vertebrados no mandibulados e invertebrados de mecanismos de inmunidad adaptativa alternos, plantea la necesidad de considerar nuevos elementos en la construcción de un modelo evolutivo de la inmunidad en animales. Algunos de esos elementos se esbozan en este ensayo.   Abstract   The immune system in animals is composed by a series of cell and molecular mechanisms that coordinately maintain the physiological and genetic integrity of the organism. Traditionally, two classes of immunity have been considered, the innate immunity and the adaptive immunity. The former is ancestral, with limited variability and low discrimination. The latter is highly variable, specific and limited to jawed vertebrates. Adaptive immunity is based on antigen receptors that rearrange somatically to generate a nearly unlimited diversity of molecules. Likely, this mechanism of somatic recombination arose as a consequence of a horizontal transfer of transposons and transposases from bacterial genomes in the ancestor of jawed vertebrates. The recent discovery in jawless vertebrates and invertebrates of alternative

  5. Componentes Principales (C.P. de los sistemas de calculo del IPC base 92 y sistema IPC base 2001

    Directory of Open Access Journals (Sweden)

    Julio Pallas González

    2002-01-01

    Full Text Available Nueve años después de que se adoptara un cambio de base en el cálculo del IPC para el Estado Español, asistimos a un cambio de sistema en el cálculo del mismo, naciendo el sistema de índices de precios, base 2001. A lo largo de este trabajo y utilizando el programa estadístico SPSS, se tratará de realizar un análisis multivariante, sobre las distintas variables que componen el IPC base 92, del estado español, y las del índice, atendiendo al nuevo sistema base 2001, que entró en vigor en enero de 2002. De todas las técnicas multivariantes susceptibles de ser aplicadas en este trabajo, se utilizaran el análisis de componentes principales y el análisis factorial, con el fin de obtener factores no observables que permitan explicar, perdiendo la mínima información, las variables originales que representan.

  6. Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems.

    Science.gov (United States)

    Kawabe, Yoshinori; Komatsu, Shinya; Komatsu, Shodai; Murakami, Mai; Ito, Akira; Sakuma, Tetsushi; Nakamura, Takahiro; Yamamoto, Takashi; Kamihira, Masamichi

    2018-05-01

    Chinese hamster ovary (CHO) cells have been used as host cells for the production of pharmaceutical proteins. For the high and stable production of target proteins, the transgene should be integrated into a suitable genomic locus of host cells. Here, we generated knock-in CHO cells, in which transgene cassettes without a vector backbone sequence were integrated into the hprt locus of the CHO genome using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 and CRISPR-mediated precise integration into target chromosome (CRIS-PITCh) systems. We investigated the efficiency of targeted knock-in of transgenes using these systems. As a practical example, we generated knock-in CHO cells producing an scFv-Fc antibody using the CRIS-PITCh system mediated by microhomology sequences for targeting. We found that the CRIS-PITCh system can facilitate targeted knock-in for CHO cell engineering. Copyright © 2017 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  7. ALMACÉN DE CARBONO EN SISTEMAS AGROFORESTALES CON CAFÉ

    Directory of Open Access Journals (Sweden)

    William Espinoza-Domínguez

    2012-01-01

    Full Text Available El presente estudio se realizó con el objetivo de estimar el almacén de carbono en sistemas agroforestales (SAF con base en café en la región de Huatusco, zona de mayor producción de café (Coffea arabiga L. en el estado de Veracruz, México, a fin de obtener información cuantitativa sobre el potencial de mitigación de carbono en los SAF de la región. Para ello, se estimaron los reservorios de carbono en biomasa vegetal y la materia orgánica edáfica existentes en sistemas agroforestales de café-plátano, café-macadamia, café-cedro rosado, café-ganado ovino y café-chalahuite, y como referencia de una condición no agroforestal se incluyeron un bosque primario y un potrero con pradera natural. La recolección de muestras se hizo en parcelas de 4 x 25 m (100 m2, donde se colectó biomasa de vegetación herbácea y mantillo, y para el suelo la profundidad de muestreo fue de 0 a 30 cm. El sistema agroforestal que presentó la mayor cantidad de carbono aéreo, estimado con modelos alelométricos, fue café-cedro rosado (Ca + Ce, con 114 Mg C·ha-1, seguido de los sistemas café - macadamia (Ca + Ma, con 34 Mg C·ha-1; café-chalahuite (Ca + Ch, con 29 Mg C·ha-1; café-plátano velillo (Ca + Pl, con 27 Mg C·ha-1; y por último el sistema silvopastoril (Sp, con 2 Mg C·ha-1. Para el caso del carbono orgánico del suelo, el tratamiento (Ca + Ce fue el que presentó mayor contenido de carbono fácilmente oxidable en el suelo, con 58 Mg C·ha-1, y el menor fue el tratamiento potrero (P, con 50 Mg C·ha-1. Del carbono orgánico total entre los SAF el tratamiento Ca + Ce fue el que tuvo el mayor valor, con 172 Mg C·ha-1, y el tratamiento silvopastoril (Sp presentó el menor, con 65 Mg C·ha-1. De los sistemas evaluados se concluye que, en promedio, los SAF almacenan 102 Mg C·ha-1, mientras que el potrero almacena 52 t C·ha-1, y el bosque primario es el sistema que más almacena carbono total, con un valor de 355 Mg C·ha-1.

  8. Seguridad social y sistemas previsionales en América Latina

    OpenAIRE

    Solano-Brenes, Ana Isable

    2016-01-01

    Este artículo pretende comunicar el resultado de una investigación sobre la Seguridad Social y los sistemas previsiones, desde sus orígenes en la edad media, su transformación en la Revolución Industrial, así como los diversos sistemas que se desarrollan hasta dar paso a los llamados Sistemas Previsionales como uno de los aspectos más importantes de la Seguridad Social.

  9. Criterios de seguridad en tableros de puentes con sistema de atirantamiento extradosado

    OpenAIRE

    Liu, Zhang

    2017-01-01

    Los criterios de seguridad en Estado Límite Último en tableros de hormigón pretensado están claramente establecidos para sistemas de pretensado convencional adherente o no, interior o exterior a la losa del tablero así como para sistemas de atirantamiento convencionales. Sin embargo en la normativa actual no se contempla criterios de seguridad aplicables a sistemas de atirantamiento no convencionales como el extradosado. El trabajo pretende analizar los criterios de segurida...

  10. Efikasnost sistema PVO u uslovima elektronskih dejstava / Efficiency of antiaircraft system in electronic warfare

    Directory of Open Access Journals (Sweden)

    Vladimir V. Šepec

    2007-01-01

    Full Text Available U radu je primenjena teorija masovnog opsluživanja u analizi efikasnosti sistema protivvazduhoplovne odbrane (sistema PVO u uslovima elektronskih dejstava protivnika. Prikazan je model za ocenu efikasnosti sistema PVO i predstavljeni su izrazi za procenu efikasnosti sistema PVO u uslovima elektronskih dejstava. / In this article the theory of mass servicing is used in the analysis of efficiency of antiaircraft system in the conditions of electronic warfare. The model for analysis of the antiaircraft system efficiency has been shown and formulas for qualitative analysis of antiaircraft system efficiency have been presented in the conditions of electronic warfare.

  11. Sistema de iluminación inteligente inalámbrico

    OpenAIRE

    Nicolás Jaro, Jesús

    2014-01-01

    Este proyecto crea un sistema de iluminación inteligente compuesto por múltiples dispositivos que, de forma inalámbrica, se sincronizan entre si, para encenderse, apagarse y cambiar el color de la luz ambiente. El sistema se ha diseñado para conectar hasta 8 dispositivos basados en el sistema embebido LPC1769 y S.O. FreeRTOS, integrando un sensor acelerómetro, varios leds de distinto color y un módulo WiFly RN-XV que los interconecta de forma inalámbrica. Aquest projecte crea un sistema d'...

  12. Implementación de los Servicios Terminal Server en un Sistema Corporativo

    OpenAIRE

    PUCHALT RODRÍGUEZ, ANA ELVIRA; GÓMEZ GARCÍA, PABLO JOSÉ

    2014-01-01

    Este proyecto final de carrera presenta el diseño e implementación de los sistemas de acceso a la red y de los servicios de Terminal Server en un sistema corporativo basado en Windows Server 2008 R2. Dado que no hay posibilidad de realizar el proyecto en un sistema corporativo real, se establecerá un sistema corporativo ficticio, donde cada uno de los trabajadores obtendrá acceso a su propia sesión con sus credenciales identificadoras. En esta sesión, se tendrá acceso a determi...

  13. Sistemas de acceso a la información telemática

    OpenAIRE

    Merlo Vega, José Antonio

    1998-01-01

    Internet ofrece distintos sistemas para acceder a la información. Se exponen los diferentes tipos de documentos que pueden ser localizados en Internet, así como las distintas fuentes de información de Internet. Se ofrece una clasificación de los sistemas de acceso a la información telemática a partir del tratamiento y el análisis que recibe la información, dividiéndose estos sistemas en tratamiento intelectual y automático y sistemas con o sin análisis documental. Se estudian los distintos as...

  14. Modelación de un sistema de reinyección de agua

    OpenAIRE

    Zamora Aizaga, Iván Marcelo; Barahona Borja, Tony Roman

    2012-01-01

    170 hojas : ilustraciones, 29 x 21 cm + CD-ROM 4531 Este trabajo está orientado a realizar una modelación en estado estable de un sistema de reinyección de agua de formación con la finalidad de obtener el punto óptimo de operación entre las curvas del sistema de bombas y la curva del sistema de redes de tuberías. El dimensionamiento de redes de tuberías y la curva del sistema de diferentes tipos de bombas operando serie o paralelo son problemas comunes en la industria petrolera, las cuales...

  15. Sistema de Aprovechamiento de la Energía de las Olas del Mar

    OpenAIRE

    Pi Amorós, Gabriel

    2010-01-01

    Este documento describe un sistema de aprovechamiento de la energía transportada por el oleaje o energía undimotriz. El sistema no transformará la energía únicamente en electricidad si no que aprovecha dicha electricidad para producir hidrógeno mediante el proceso de la electrólisis del agua de mar. Es decir, el sistema propuesto es un sistema de generación de hidrógeno de origen renovable a partir de una fuente de energía renovable, la energía undimotriz. Las característica...

  16. Desarrollo de un sistema de asignación de tareas a trabajadores polivalentes

    OpenAIRE

    Mora Jardón, Alejandro; Moreno Medina, Irene

    2007-01-01

    El proyecto consiste en: analizar las necesidades de gestión de información en la asignación de tareas. Diseño de un sistema de gestión para esta asignación. Desarrollo de una aplicación para aplicar este sistema. Pruebas del sistema sobre diversos ejemplos. Este trabajo de final de carrera consiste en la creación y posterior desarrollo de un sistema de asignación de tareas a trabajadores polivalentes. Esto es, una herramienta que nos organice la distribución laboral a lo largo...

  17. Construindo um modelo de sistema de cuidados Construyendo un modelo de sistema de cuidados Developing an explanatory theoretical model of system of care

    Directory of Open Access Journals (Sweden)

    Alacoque Lorenzini Erdmann

    2007-06-01

    Full Text Available OBJETIVO: Construir um modelo teórico explicativo de Sistema de Cuidados. METODO: Estudo exploratório qualitativo apoiado nos pressupostos da Grounded Theory. Participaram do estudo 15 profissionais e os dados foram coletados por entrevista aberta. RESULTADOS: O Modelo teórico foi delimitado a partir do fenômeno Construindo o Sistema Complexo de Cuidados determinado pelas categorias: Convivendo com a dinamicidade do sistema de cuidados e Organizando o sistema de cuidados a partir de múltiplas interações. CONCLUSÃO: O modelo de sistema de cuidados apresentou-se como sistema vital e dinâmico construído a partir de redes interacionais de vários saberes do agir humano expresso pelo trabalho compartilhado, multidisciplinar e em equipe.OBJETIVO: Construir un modelo teórico explicativo de Sistema de Cuidados. MÉTODO: Se trata de un estudio cualitativo exploratorio apoyado en las premisas de la Teoría Fundamentada en los Datos. Participaron del estudio 15 profesionales. Los datos fueron recolectados por medio de una entrevista abierta. RESULTADOS: El modelo teórico se delimitó al fenómeno Construyendo el Sistema Complejo de Cuidados determinado por las categorías: Viviendo junto con la dinamicidad del sistema de cuidados y Organizando el sistema de cuidados a partir de interacciones múltiples. CONCLUSIÓN: El modelo de sistema de cuidados se presenta como un sistema vital y dinámico construido a partir de redes de interacción de conocimientos del actuar humano expresado en el trabajo conjunto, multidisciplinario y en equipo.OBJECTIVE: To develop an explanatory theoretical model of the system of care. METHOD: Grounded theory served as the conceptual framework to conduct this qualitative exploratory study. Unstructured interviews were conducted among 15 care professionals. RESULTS: The theoretical model was delimited from the phenomenon "building complex system of care" as determined by the following categories: "dealing with a

  18. Las organizaciones de enfermería como sistemas complejos

    Directory of Open Access Journals (Sweden)

    Caro Castillo Clara Virginia

    2002-10-01

    Full Text Available

    This study takes into account some considerations about the classical logic when building knowledge. It states the logic of complexity, the complex thought and the rational, open and complex systems, as a new paradigm. In the light of the organization concept proposed by Morin, complex adaptative systems are emphasized, as well as other terms such as order, disorder, incidental events and noises. The self-eco-organizer process, takes the human being thinking as starting point and as dynamizer of simple and complex processes in the construction of knowledge about reality. Some research studies such as those of Erdmann, Meirelles and Pradebon show some ways in which one can learn from the organizational reality of nursing, the exercise of complex thinking in uncertainty, in tenous limits, in heterogeneity, diversity and pluralism of phenomena in its aspect, interactions and associations. The inter subjectivity states a new concept of space and of temporality. The care system organization in nursing visualizes several dimensions of "caríng": to care for himself, to care for himself together with the other, to be looked after by the other, to feel and the process of personal care system, the body itself as the nucleus of care, the fact of belonging to the system of multiple caring relations and the care for nature getting integrated to other beings and other social, natural systems, and in thisway strengthen the feeling of ownership, in search of a better survival, vitality, life human quality.

    El presente estudio hace algunas consideraciones sobre la lógica clásica en la construcción del conocimiento. Señala la lógica de la complejidad, el pensamiento complejo y los sistemas racionales, abiertos y complejos como un nuevo paradigma. A la luz de la concepción de organización propuesta por Morin, enfatiza los sistemas adaptativos complejos, el orden, desorden, eventos, incidentes y ruidos. El proceso auto-eco-organizador toma como

  19. El Sistema Bethesda 2001 The 2001 Bethesda System

    Directory of Open Access Journals (Sweden)

    Christine Bergeron

    2003-01-01

    Full Text Available The Bethesda system is a system of terminology for reporting the results of cervical cytology. It was developed in 1988, and is now widely used in the United States. This system was updated in April 2001. The most important modifications are the following: a elimination of the category "satisfactory but limited by", b the reintegration of benign modifications in the normal category, c "atypical squamous cells of undetermined significance" is now named "atypical squamous cells" and subdivided into "atypical squamous cells of undetermined significance" and "cannot exclude high grade squamous intraepithelial lesion"; and d endocervical adenocarcinoma in situ which is now a separate entity. Low grade squamous intraepithelial lesions and high grade squamous intraepithelial cells remain unchanged. Recommendations have been proposed concerning automated review and ancillary testing.El Sistema Bethesda es un sistema de terminología para informar los resultados de citología cervical. Se inició en 1988 y en la actualidad se usa en todos los Estados Unidos de América. Este sistema se actualizó en abril de 2001. Las modificaciones más importantes son las siguientes: a eliminación de la categoría "satisfactorio pero limitado por", b se reintegraron las modificaciones benignas en la categoría normal, c la categoría "células escamosas atípicas de significado indeterminado" ahora se denomina "células escamosas atípicas", y se subdivide en "células escamosas atípicas de significado indeterminado" y "no se puede excluir una lesión escamosa de alto grado", y d adenocarcinoma endocervical in situ, que ahora es una entidad separada. Las lesiones escamosas intraepiteliales de bajo y alto grado quedan sin cambios. Se proponen recomendaciones relacionadas con el uso de la revisión automatizada y la utilización de pruebas auxiliares.

  20. Sistema de salud de Ecuador The health system of Ecuador

    Directory of Open Access Journals (Sweden)

    Ruth Lucio

    2011-01-01

    Full Text Available En este trabajo se describen las condiciones de salud en Ecuador y, con mayor detalle, las características del sistema ecuatoriano de salud, incluyendo su estructura y cobertura, sus fuentes de financiamiento, los recursos físicos, materiales y humanos de los que dispone, las tareas de rectoría que desarrolla el Ministerio de Salud Pública, la generación de información en salud, las tareas de investigación, y la participación de los ciudadanos en la operación y evaluación del sistema. También se discuten las innovaciones más recientes que se han implantado en el sistema ecuatoriano de salud dentro de las que destaca la incorporación de un capítulo específico sobre salud a la nueva Constitución que reconoce a la protección de la salud como un derecho humano y la construcción de la Red Pública Integral de Salud.This paper describes the health conditions in Ecuador and, in more detail, the characteristics of the Ecuadorian health system, including its structure and coverage, its financial sources, the physical, material and human resources available, and the stewardship activities developed by the Ministry of Public Health. It also describes the structure and content of its health information system, and the participation of citizens in the operation and evaluation of the health system. The paper ends with a discussion of the most recent policy innovations implemented in the Ecuadorian system, including the incorporation of a chapter on health into the new Constitution which recognizes the protection of health as a human right, and the construction of the Comprehensive Public Health Network.

  1. SISTEMA INMUNE Y VACUNACIÓN DE PECES

    Directory of Open Access Journals (Sweden)

    GIOVANNY PENAGOS

    2009-01-01

    Full Text Available RESUMEN Los peces poseen un sistema inmune con muchas de las células y sustancias humorales presentes en vertebrados superiores, pero cuentan también con componentes y funciones especiales, como los centros melanomacrófagos y la capacidad fagocítica de los enterocitos por citar solo algunas de ellas, que difieren profundamente con sus similares en otras especies y que aún hoy son pobremente comprendidas. Sumado a esto, el ambiente acuático y más, el ambiente acuático productivo, es de por si complejo de manera que las posibilidades de las interacciones biológicas son enormes y los procesos difícilmente predecibles. La vacunación es tal vez una de las herramientas más importantes para el control de enfermedades bacterianas en peces, no solo por su potencial preventivo y correctivo sino también por sus bondades con el ambiente y con la salud pública que contrastan notoriamente con los tratamientos antibióticos. El éxito de las vacunas en especies piscícolas depende en buena medida del conocimiento adecuado del sistema inmunológico de los peces, de las interacciones hospedero-ambiente-patógeno, así como de las particularidades de los sistemas productivos. Con todo, la vacunación en ambientes acuáticos se enfrenta a un sinnúmero de dificultades que deben ser abordadas antes que con un enfoque unilateral farmacológico, con una aproximación integral en la que se incluye el uso de herramientas epidemiológicas, clínicas, patológicas, microbiológicas, etc. Palabras clave: vacunación, peces, inmunidad de peces, enfermedades infecciosas en peces.

  2. SISTEMAS TUTORES INTELIGENTES COMO APOYO EN EL PROCESO DE APRENDIZAJE

    Directory of Open Access Journals (Sweden)

    Edwin Eduardo Millan Rojas

    2015-09-01

    Full Text Available El objetivo principal de este artículo es la identificación de las principales características de los tutores inteligentes, su origen y evolución los cuales pueden ofrecer elementos para la formulación de nuevos proyectos de investigación relacionados con la educación y el uso de los tutores. El método utilizado es el descriptivo y sistémico, los cuales permiten recopilar los datos necesarios, la información presentada ha sido adquirida de bases de datos especializadas como IEEE, Redalyc, ACM y Science Direct, además de artículos de revistas de universidades internacionales y de proyectos de investigación descargados de Google Académico. Los Sistemas Tutores Inteligentes surgieron al combinar técnicas de inteligencia artificial (IA con los métodos clásicos de enseñanza. Estos son sistemas de software que generan un ambiente idóneo para la interacción y satisfacción de las necesidades del estudiante, adaptable a los conocimientos previos y a la capacidad de evolución de cada alumno respecto a un área del conocimiento. El funcionamiento de los STI se basa en la relación entre tres principales módulos (tutor, estudiante y dominio, para presentar al usuario la información de manera estructurada. La investigación llevada a cabo permitió reunir los aspectos más relevantes de los Sistemas Tutores Inteligentes (STI y presentarlos como una herramienta óptima para llevar a cabo un proceso de aprendizaje.

  3. El sistema educativo y el trabajo en Venezuela

    OpenAIRE

    Morillo Moreno, Marysela C.

    2009-01-01

    El sistema educativo y en el mercado laboral se trabaja con el más importante y valioso recurso: el humano, el cual presenta unas características muy peculiares que merecen ser estudiadas como una rama de la economía como lo es la economía de la educación y la economía laboral. En Venezuela, ambas ramas han sido poco abordadas, pues otros temas económicos han ocupado las reflexiones; sin embargo, las cifras de desempleo y los problemas sociales son cada vez más alarmantes, convirtiéndose en l...

  4. El parentesco como sistema en la interfaz bio-cultural

    OpenAIRE

    G??mez Garc??a, Pedro

    2011-01-01

    Aqu??, he centrado mi investigaci??n en la descripci??n del sistema de parentesco humano como constituido por una organizaci??n espec??fica, en la que se opera una articulaci??n bio-cultural. El parentesco no consiste solo en elementos biol??gicos, o m??s exactamente gen??ticos, ni tampoco ??nicamente en los determinantes sociales o culturales. Las relaciones familiares se constituyen y desarrollan en la interfaz entre el plano biogen??tico y el sociocultural, dando lugar a la formaci??n del ...

  5. SISTEMA SCADA PARA EL PROCESO DE PASTEURIZACION DE JUGOS

    Directory of Open Access Journals (Sweden)

    Juan de Dios Contreras Cáceres

    2014-06-01

    Full Text Available La finalidad de este artículo es dar a conocer el proyecto desarrollado para diseñar e implementar un sistema SCADA (Supervisory Control And Data Adquisition aplicado al proceso de pasteurización de jugos. Se siguieron las etapas de diseño, partiendo de la elaboración del esquemático Layout, la programación del PLC, los drivers de comunicación y el diseño del software SCADA para una planta de jugos.

  6. Control de trazabilidad de fruta mediante un sistema RFID

    OpenAIRE

    Asensio Nácher, Sergi

    2015-01-01

    En este proyecto se ha desarrollado una aplicación para realizar la trazabilidad de la fruta en un almacén utilizando dispositivos de radiofrecuencia RFID. El sistema ha sido desarrollado en C\\# y ha permitido automatizar todo el proceso de control de la fruta en un almacén. In this project, has been developed an application for making the traceability of fruit in a warehause by using radiofrequency systems RFID. The system was developed in C\\# and permits to control de processes of the fr...

  7. La reforma del sistema financiero peruano, 1990-1995

    OpenAIRE

    Jorge Rojas

    1994-01-01

    El presente trabajo es la primera parte de una investigación sobre la reforma del sistema financiero peruano y busca, básicamente, hacer una descripción del proceso de reforma en sí, mientras que la segunda parte, aún en elaboración, tratará de evaluar los resultados de la reforma, haciendo uso extenso de estadísticas financieras que en esta primera parte no se trabajan. Lo que buscamos, entonces, es poder dar una idea clara de los cambios en las reglas de juego producidos por la reforma y de...

  8. Sistemas integrados de gestão empresarial

    OpenAIRE

    Schmitt, Carlos Alberto

    2004-01-01

    Tese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia de Produção. Para ser competitiva e acompanhar ou liderar a concorrência, uma empresa necessita empregar a tecnologia da informação. Neste contexto as empresas estão implementando ERP para gerenciar os seus recursos, fornecer serviços e produtos úteis aos clientes e garantir a integração de seus sistemas. Para alcançar o sucesso na implementação de um ERP e os resultados d...

  9. SISTEMA WEB DE GESTIÓN DE RECURSOS HUMANOS

    OpenAIRE

    Peralta Rojas, Andrés Alejandro; Solorzano Sarmiento, David Alberto

    2015-01-01

    El departamento de Recursos Humanos de las Empresas llevan a cabo la gestión y control de los empleados manualmente (archivos impresos, documentos de Word y Excel) lo que conlleva a problemas como: gestión de trámites, pago de salarios no realizados a tiempo, poca comunicación con los empleados, deterioro de información laboral de los empleados archivados por largo tiempo. Para solucionar este problema nos basamos en la creación de un sistema web d...

  10. Mantenimiento de Sistemas de Gestión Integrados

    OpenAIRE

    Rodríguez Rodríguez, Irene

    2013-01-01

    El Trabajo de Fin de Master permite aplicar de forma práctica los conocimientos adquiridos durante el curso del Master Oficial de la Prevención de Riesgos Laborales, Calidad y Medio Ambiente, impartido en la Universidad de Valladolid. La presente memoria técnica resume los requisitos mínimos para realizar un adecuado mantenimiento de un sistema de gestión integrado; para ello, se citan los distintos puntos a tener en cuenta (recursos humanos, formación,requisitos legales...), acompañados de e...

  11. Development of solar thermophotovoltaic systems = Desarrollo de sistemas termofotovoltaicos solares

    OpenAIRE

    Datas Medina, Alejandro

    2011-01-01

    Esta tesis aborda el análisis, tanto teórico como experimental, de los sitemas termofotovoltaicos solares. En estos sistemas, un material (emisor) se calienta hasta la incandescencia mediante radiaci ón solar. La radiación térmica emitida por dicho material se dirige hacia una célula fotovoltaica, que convierte dicha radiación en electricidad. En esta configuración, se pueden emplear elementos de control espectral para lograr que los fotones no útiles para el proceso de conversión fotovoltáic...

  12. Pumping systems characterization; Caracterizacion de los sistemas de bombeo

    Energy Technology Data Exchange (ETDEWEB)

    Torres Gutierrez, R. [Eficiencia Energetica, S.A. de C.V., Mexico, D. F. (Mexico)

    1995-12-31

    In this paper the characterization of the pumping systems is discussed, and an explanation is given of the need of evaluating in detail the possibility of implementing the frequency variators as an option for energy conservation, explaining how the simple use of the similarity or affinity laws lead to over-estimate the saving. [Espanol] En este articulo se describe la caracterizacion de los sistemas de bombeo y se explica la necesidad de evaluar en detalle la posibilidad de implementacion de variadores de frecuencia, como una alternativa para el ahorro de energia, explicando como el simple uso de las leyes de semejanza o afinidad llevan a sobre-evaluar los ahorros.

  13. Pumping systems characterization; Caracterizacion de los sistemas de bombeo

    Energy Technology Data Exchange (ETDEWEB)

    Torres Gutierrez, R [Eficiencia Energetica, S.A. de C.V., Mexico, D. F. (Mexico)

    1996-12-31

    In this paper the characterization of the pumping systems is discussed, and an explanation is given of the need of evaluating in detail the possibility of implementing the frequency variators as an option for energy conservation, explaining how the simple use of the similarity or affinity laws lead to over-estimate the saving. [Espanol] En este articulo se describe la caracterizacion de los sistemas de bombeo y se explica la necesidad de evaluar en detalle la posibilidad de implementacion de variadores de frecuencia, como una alternativa para el ahorro de energia, explicando como el simple uso de las leyes de semejanza o afinidad llevan a sobre-evaluar los ahorros.

  14. Sistemas de Registro Audiovisual del Patrimonio Urbano (SRAPU)

    OpenAIRE

    Conles, Liliana Eva

    2006-01-01

    El Sistema SRAPU es un método de relevamiento fílmico diseñado para configurar una base de datos interactiva del paisaje urbano. Sobre esta base se persigue la formulación de criterios ordenados en términos de: flexibilidad y eficacia económica, eficiencia en el manejo de datos, democratización de la información. El SRAPU se plantea como un registro audiovisual del patrimonio material e intangible en su singularidad y como conjunto histórico y natural. En su concepción involucra los pro...

  15. Modelo para sistemas da qualidade como base da estrategia competitiva

    OpenAIRE

    Marco Antonio Silveira

    1999-01-01

    Resumo: Vários autores propõem a empresa como um sistema em transformação, sendo que esse enfoque sistêmico parece oferecer um meio efetivo de prover as capacidades estratégicas que são valorizadas pelos mercados atuais. Por outro lado, vem crescendo a consciência para o enfoque da qualidade como base da estratégia competitiva, onde esta é associada à lucratividade e incluída no processo de planejamento estratégico, tornando-se um instrumento eficaz de concorrência que, para fazer frente à di...

  16. SISTEMAS DE EVALUACION DE CONTENIDOS EN TELEFORMACION: EL PROYECTO CFV

    Directory of Open Access Journals (Sweden)

    Rosa Maria Bo

    1999-08-01

    Full Text Available La evaluación del rendimiento de los alumnos en los sistemas basados en teleformación es uno de los temas claves como en cualquier programa de formación. Este proceso presenta las características básicas comunes con los restantes programas de formación y añade las propias de este acercamiento. Vamos a revisar algunas de las mismas, situándolas dentro de la experiencia y alternativas que actualmente están disponibles y, particularmente, desde la perspectiva que a este respecto se desarrolla en el proyecto CFV

  17. Adam Smith y Sistemas Dinámicos

    Directory of Open Access Journals (Sweden)

    Osvaldo Acuña

    2008-01-01

    Full Text Available Presentamos un sistema dinámico sencillo con dos ecuaciones diferenciales, una representa la trayectoria del precio y la otra la de la cantidad de un bien en una economía. Nuestro modelo se basa sobre todo en la teoría clásica de precios expuesta por Adam Smith en La Riqueza de las Naciones. Al final del trabajo, se presenta, a modo de ejemplo, un estudio para el caso del ganado vacuno en la economía mundial.

  18. Envolvimento nos direitos humanos e sistemas de valores

    OpenAIRE

    Pereira,Cícero; Ribeiro,Ana Raquel Correia; Cardoso,Sandro José

    2004-01-01

    Com base no modelo da análise quantitativa das representações sociais, o estudo (N = 300) tratou do posicionamento de estudantes universitários em relação aos Direitos Humanos (DHs) e à ancoragem social desse posicionamento nos sistemas de valores dos estudantes. Os resultados mostram que as representações dos estudantes sobre o envolvimento nos DHs envolvem quatro princípios organizadores: pessoal-abstrato; pessoal-concreto; governamental-abstrato; governamental-concreto. Em relação à ancora...

  19. Sistemas de gestión urbana regional

    OpenAIRE

    Biere Arenas, Rolando Mauricio

    2009-01-01

    Este proyecto busca compartir prácticas exitosas y uso de tecnologías avanzadas en la gestión urbana regional, a través del intercambio y formación conjunta entre instituciones de España, México y Chile que desarrollan este tema. Este trabajo expone la síntesis de la propuesta denominada Sistemas de Gestión Urbana Regional, desarrollada en el marco de la Convocatoria 2009 de la Agencia Española de Cooperación Internacional para el Desarrollo de Proyectos de Cooperación para Iberoamérica....

  20. Cloud Computing en salud: Sistema para Administrar Imagenes Biomedicas

    OpenAIRE

    Alonso Calvo, Raul; Crespo del Arco, Jose; Maojo Garcia, Victor Manuel; Muñoz, Alberto; García Rojo, Marcial; Perez Jimenez, Libia; Azpiazu Cuenca, Javier Salvador

    2011-01-01

    En el campo de la biomedicina se genera una inmensa cantidad de imágenes diariamente. Para administrarlas es necesaria la creación de sistemas informáticos robustos y ágiles, que necesitan gran cantidad de recursos computacionales. El presente artículo presenta un servicio de cloud computing capaz de manejar grandes colecciones de imágenes biomédicas. Gracias a este servicio organizaciones y usuarios podrían administrar sus imágenes biomédicas sin necesidad de poseer grandes recursos informát...

  1. VZPOSTAVITEV SISTEMA ZA UPRAVLJANJE INFORMACIJSKE VARNOSTI V PROIZVODNEM PODJETJU

    OpenAIRE

    Škarja, Peter

    2012-01-01

    Magistrska naloga obravnava vzpostavitev sistema za upravljanje informacijske varnosti (SUIV) v proizvodnem podjetju, pri čemer se osredotoča na 1. fazo Demingovega kroga – načrtovanje vzpostavitve SUIV. Sestavljena je iz dveh delov. V prvem delu so podane teoretične osnove s področja informacijske varnosti. Prikazana je zasnova modela načrtovanja SUIV s pomočjo smernic, ki jih podaja standard ISO/IEC 27003. V drugem, praktičnem delu je predstavljeno podjetje Vega International d.o.o. Opisano...

  2. PRIPRAVA STROKOVNIH PODLAG ZA UVEDBO SISTEMA ZA UPRAVLJANJE INFORMACIJSKE VARNOSTI

    OpenAIRE

    Tršelič, Tugomir

    2014-01-01

    V diplomskem delu smo raziskali področje informacijske varnosti. Namen raziskave je bil priprava strokovnih podlag, na osnovi katerih smo pridobili podporo vodstva za vpeljavo sistema upravljanja z informacijsko varnostjo v podjetju. V raziskavo smo vključili ključne koncepte informacijske varnosti, pregled novih tehnologij, opravljene lokalne in globalne raziskave s področja varnostnih incidentov in pregled pomembne področne zakonodaje. Ugotovitve smo nadgradili s pregledom ključnih področni...

  3. Gestión de Procesos en los Sistemas Operativos

    OpenAIRE

    Serrano Castaño, Francisco Javier

    2011-01-01

    Comparación de los sistemas operativos Windows 7 Profesional, Ubuntu 10.10 Desktop y Mac OS X Snow Leopard Desktop respecto al rendimiento que ofrecen sobre el servidor web Apache instalado en cada uno de ellos. Comparació dels sistemes operatius Windows 7 Professional, Ubuntu 10.10 Desktop i Mac OS X Snow Leopard Desktop respecte al rendiment que ofereixen sobre el servidor web Apache instal lat en cada un d'ells. Comparison of Windows 7 Professional operating systems, Ubuntu 10.10 ...

  4. ODLOČITVENI MODEL ZA IZBIRO INFORMACIJSKEGA SISTEMA GOSTINSKEGA LOKALA

    OpenAIRE

    Radišić, Nikola

    2011-01-01

    Diplomska naloga vsebuje obravnavo procesa odločanja v kontekstu poslovnih sistemov. Osredotočenje je na računalniškem programu za podporo odločanju Dexi in reševanju odločitvenega problema izbire informacijskega sistema gostinskega lokala (GIS). Za reševanje problema uporabljena je metoda modeliranja večkriterijskega odločitvenega modela v DEXi-ju, kjer so za slučaj gostinskega lokala "Flirt Sushi Longue" ovrednoteni pet variant (ponudnikov GIS-ov v Sloveniji). Rezultat diplomskega dela je a...

  5. Sistemas visuales en identidades dinámicas

    OpenAIRE

    Lorenz, Martin

    2016-01-01

    En este momento el mundo del diseño gráfico está experimentando cambios relevantes motivados por el momento social que estamos viviendo, con todo el sistema económico inmerso en una crisis que afecta a las empresas y los consumidores, las redes sociales que transforman nuestra manera de comunicarnos y las nuevas tecnologías que nos permiten comunicarnos diferente. Este contexto está promoviendo cambios en la propia profesión del diseñador gráfico y la manera en cómo diseñamos identidades visu...

  6. PROPIEDADES ELECTRONICAS DE SISTEMAS BASADOS EN GRAFENO BICAPA

    OpenAIRE

    SUAREZ MORELL; ERIC; SUAREZ MORELL; ERIC

    2011-01-01

    La estructura electrónica de una monocapa de Carbono fue estudiada inicialmente[l ] como una primera aproximación al estudio de las propiedades del Grafito, sin embargo no fue sino hasta el 2004 que fue sintetizado experimentalmente[2]. La monocapa de Carbono ha recibido el nombre en la literatura de Grafeno palabra proveniente del Grafito y del Fullereno, otro de los alotropos del Carbono. A partir del 2004 el estudio de sistemas basados en Grafeno se ha convertido en uno de l...

  7. Autoevaluación del Sistema de Control Interno Autoevaluación del Sistema de Control Interno

    Directory of Open Access Journals (Sweden)

    Rubí Consuelo Mejía Quijano

    2005-06-01

    Full Text Available La autoevaluación del control es un elemento del Sistema de Control Interno que permiteel diagnóstico y el fortalecimiento organizacional. Se desarrolla en toda la entidad y supone el apoyo de la alta dirección, apoyo representado en recursos físicos y la orientación que lebrinde durante su aplicación.Dicho elemento genera mayor responsabilidad en los empleados, al involucrarlos en el análisisde fortalezas y debilidades del sistema de control, al comprometerlos con la recolección de la información que soporta el juicio sobre el estado del sistema y al permitirles proponer planesde mejoramiento que contribuyan al logro del objetivo del sistema de control, y por ende alde la organización.Las dificultades en su aplicación pueden provenir de aspectos relacionados con la culturaorganizacional: si el personal no está dispuesto a colaborar con los grupos de apoyo en elsuministro de la información, si existe apatía con el proceso y si no se cuenta con personas capacitadas para realizarlo.El proceso se efectúa en tres etapas; la primera consiste en la planeación, en la que el Comité de Autoevaluación compromete la gerencia y se asignan actividades y responsabilidades; sedefine el cronograma de trabajo, los recursos necesarios y se capacita a los grupos de apoyo;la segunda se refiere a la ejecución, consistente en el levantamiento de la información que soporta el análisis de cada uno de los elementos del sistema de control y permite emitir juicios objetivos sobre su desempeño; finalmente, la tercera, elaboración del informe, da cuenta delos resultados de la autoevaluación del sistema de control, de su desarrollo y sus dificultades, y establece las propuestas de mejora.Control Self-Assessment is an element of the Internal Control System which allows the organizational diagnose and strengthening. It is run throughout the entity and supposes the support from the high managerial circle. This support is in the form of physical

  8. Extending the half-life of a fab fragment through generation of a humanized anti-human serum albumin Fv domain: An investigation into the correlation between affinity and serum half-life.

    Science.gov (United States)

    Adams, Ralph; Griffin, Laura; Compson, Joanne E; Jairaj, Mark; Baker, Terry; Ceska, Tom; West, Shauna; Zaccheo, Oliver; Davé, Emma; Lawson, Alastair Dg; Humphreys, David P; Heywood, Sam

    2016-10-01

    We generated an anti-albumin antibody, CA645, to link its Fv domain to an antigen-binding fragment (Fab), thereby extending the serum half-life of the Fab. CA645 was demonstrated to bind human, cynomolgus, and mouse serum albumin with similar affinity (1-7 nM), and to bind human serum albumin (HSA) when it is in complex with common known ligands. Importantly for half-life extension, CA645 binds HSA with similar affinity within the physiologically relevant range of pH 5.0 - pH 7.4, and does not have a deleterious effect on the binding of HSA to neonatal Fc receptor (FcRn). A crystal structure of humanized CA645 Fab in complex with HSA was solved and showed that CA645 Fab binds to domain II of HSA. Superimposition with the crystal structure of FcRn bound to HSA confirmed that CA645 does not block HSA binding to FcRn. In mice, the serum half-life of humanized CA645 Fab is 84.2 h. This is a significant extension in comparison with Fab variant. The Fab-HSA structure was used to design a series of mutants with reduced affinity to investigate the correlation between the affinity for albumin and serum half-life. Reduction in the affinity for MSA by 144-fold from 2.2 nM to 316 nM had no effect on serum half-life. Strikingly, despite a reduction in affinity to 62 µM, an extension in serum half-life of 26.4 h was still obtained. CA645 Fab and the CA645 Fab-HSA complex have been deposited in the Protein Data Bank (PDB) with accession codes, 5FUZ and 5FUO, respectively.

  9. ScFv anti-heparan sulfate antibodies unexpectedly activate endothelial and cancer cells through p38 MAPK: implications for antibody-based targeting of heparan sulfate proteoglycans in cancer.

    Directory of Open Access Journals (Sweden)

    Helena C Christianson

    Full Text Available Tumor development requires angiogenesis and anti-angiogenic therapies have been introduced in the treatment of cancer. In this context, heparan sulfate proteoglycans (HSPGs emerge as interesting targets, owing to their function as co-receptors of major, pro-angiogenic factors. Accordingly, previous studies have suggested anti-tumor effects of heparin, i.e. over-sulfated HS, and various heparin mimetics; however, a significant drawback is their unspecific mechanism of action and potentially serious side-effects related to their anticoagulant properties. Here, we have explored the use of human ScFv anti-HS antibodies (αHS as a more rational approach to target HSPG function in endothelial cells (ECs. αHS were initially selected for their recognition of HS epitopes localized preferentially to the vasculature of patient glioblastoma tumors, i.e. highly angiogenic brain tumors. Unexpectedly, we found that these αHS exhibited potent pro-angiogenic effects in primary human ECs. αHS were shown to stimulate EC differentiation, which was associated with increased EC tube formation and proliferation. Moreover, αHS supported EC survival under hypoxia and starvation, i.e. conditions typical of the tumor microenvironment. Importantly, αHS-mediated proliferation was efficiently counter-acted by heparin and was absent in HSPG-deficient mutant cells, confirming HS-specific effects. On a mechanistic level, binding of αHS to HSPGs of ECs as well as glioblastoma cells was found to trigger p38 MAPK-dependent signaling resulting in increased proliferation. We conclude that several αHS that recognize HS epitopes abundant in the tumor vasculature may elicit a pro-angiogenic response, which has implications for the development of antibody-based targeting of HSPGs in cancer.

  10. sistemas de producción tipo kanban: Descripción, componentes, diseño del sistema, y bibliografía relacionada

    Directory of Open Access Journals (Sweden)

    Oscar Javier Parra Ortega

    2013-06-01

    Full Text Available El presente artículo tiene como objeto de estudio, los sistemas de producción tipo ensamble (tree structure tipo Kanban, mono-producto, con tiempos de procesamiento aleatorios para las estaciones que lo conforman, y con capacidad limitada de producción para cada estación. Adicionalmente, se restringe el análisis de dichos sistemas, para horizontes finitos de producción. Después de una descripción del mecanismo de operación de un sistema Kanban simple, de los elementos que los componen, y del estado del arte en sistemas de producción tipo Kanban, se propone e implementa un modelo heurístico para determinar el número de kanbans, minimizando el costo promedio del inventario en proceso.

  11. Sistema de apoio a decisão com modelo aditivo para priorização de sistemas de informação

    Directory of Open Access Journals (Sweden)

    Marina Duarte

    2005-07-01

    Full Text Available As organizações estão inseridas em um ambiente dinâmico, tornando cada vez mais relevante a utilização de Sistemas de Informação que as permitam gerir eficazmente seus processos e tomar decisões. Os Sistemas de Informação, dessa forma, exercem grande impacto na competitividade das empresas e, portanto, devem estar alinhados com a estratégia do negócio da organização. A maior dificuldade, todavia, reside na priorização dos módulos de Sistemas de Informação, que deve ser feita em função das limitações de recursos. Esse artigo propõe a utilização de um Sistema de Apoio a Decisão para essa etapa do planejamento de SI, baseado num modelo aditivo.

  12. LA INFORMACIÓN DOCUMENTAL PARA LA IMPLEMENTACIÓN DE SISTEMAS DE GESTIÓN DE CALIDAD APLICANDO LA METODOLOGÍA DE SISTEMAS BLANDOS

    Directory of Open Access Journals (Sweden)

    Juan Miguel Castillo Fonseca

    2011-03-01

    Full Text Available Las necesidades de las organizaciones para conseguir eficacia, eficiencia y mejora continua en todas sus funciones y actividades que requieren el uso de documentos, conlleva a replantear las estrategias de acción e implementar sistemas de gestión de calidad que les ayuden a entender su comportamiento, su entorno y necesidades de información, tendientes a la calidad de sus procesos, productos y servicios. El presente trabajo plantea y describe, desde la concepción teórica de los sistemas y de la metodología de sistemas blandos (Soft Systems Methodology, SSM, las bases teóricas así como las ventajas y beneficios al utilizar este tipo de metodologías, que involucran a los archivos como la entidad vital que permite documentar y evidenciar todos los procesos de implementación, mantenimiento y evaluación de dichos sistemas.

  13. El Sistema Integral de Desarrollo Profesional Educativo (SíPROFE): Una mirada desde los involucrados en el sistema

    OpenAIRE

    Portaluppi Castro, Carolina

    2012-01-01

    El estudio aborda la calidad del Sistema Nacional de Desarrollo Profesional Educativo -SíPROFE- desde la perspectiva de los actores involucrados y sus distintos roles: decisor político, decisor técnico, operador de la política en el territorio, experto, instructor y docentes participantes que ejercen su práctica en contextos urbanos y rurales del cantón Guayaquil. Se trata de un estudio exploratorio y cualitativo desde la mirada de la teoría fundamentada en los datos (TFD) cuyo instrumento pr...

  14. Propuesta para establecer un sistema de vigilancia de contaminantes ambientales en Colombia

    Directory of Open Access Journals (Sweden)

    Jancy Andrea Huertas

    2015-08-01

    En conclusión, Colombia cuenta con los recursos técnicos y normativos para desarrollar el sistema de vigilancia en salud ambiental. La acción articulada de los diferentes sectores asegura la idoneidad del sistema para orientar acciones costo-efectivas en salud ambiental.

  15. Ingeniería de los sistemas de producción

    OpenAIRE

    Estrems Amestoy, Manuel

    2012-01-01

    Texto guía de la asignatura "Ingeniería de los Sistemas de Producción" que se imparte en 3er curso del título Grado en Ingeniería Química de la Universidad Politécnica de Cartagena Ingeniería de los Sistemas de Producción

  16. El feudalismo político del sistema confesional libanés

    OpenAIRE

    Hana Jalloul

    2008-01-01

    Este articulo trata de desentrañar la dificultad del complejo sistema confesional libanés, un sistema obsoleto que a día de hoy sigue condicionando la vida de la población libanesa a nivel religioso, político, social y económico.

  17. Sobre las soluciones positivas de sistemas de polinomios parametrizados en biología

    DEFF Research Database (Denmark)

    Feliu, Elisenda

    2016-01-01

    La evolución en el tiempo de las concentraciones de las especies químicas en una red de reacciones se modela comúnmente con un sistema de ecuaciones diferenciales polinomiales, bajo la denominada \\emph{ley de acción de masas}. Dichos sistemas dependen de numerosos parámetros cuyo valor es habitua...

  18. Resurrection Symphony: "El Sistema" as Ideology in Venezuela and Los Angeles

    Science.gov (United States)

    Fink, Robert

    2016-01-01

    The explosive growth of Venezuela's "El Sistema" is rewriting the agenda of musical education in the West. Many commentators from the world of classical music react to the spectacle of dedicated young colonial musicians playing European masterworks as a kind of "miracle," accepting "Sistema" founder José Antonio…

  19. mlb : Sistema de gestión de seguridad de la información

    OpenAIRE

    Lora Barbosa, Mauricio

    2014-01-01

    Memoria del sistema de gestión de seguridad de la información (SGSI) para una empresa aseguradora. Memòria del sistema de gestió de seguretat de la informació (SGSI) per a una empresa asseguradora. Master thesis for the ICT Security management program.

  20. Struggling for Integration: Universalist and Separatist Discourses within El Sistema Sweden

    Science.gov (United States)

    Bergman, Åsa; Lindgren, Monica; Saether, Eva

    2016-01-01

    This article focuses on the El Sistema programme, which started up in Sweden in 2010 with the objective to deal with segregation problems typical for Swedish urban areas. The purpose of the article is to examine how promoting integration through music and music education is constructed within El Sistema as a way to help children growing up in…

  1. Hatching Plans: Pedagogy and Discourse within an El Sistema-Inspired Music Program

    Science.gov (United States)

    Dobson, Nicolas

    2016-01-01

    In this article, I draw on my experience as an instrumental tutor with a music program inspired by and explicitly linked to El Sistema, to explore new perspectives on Sistema-based pedagogy and management. Detailed ethnographic description of an orchestral session provides a first-hand account of the program's pedagogy, which I then contextualize…

  2. Lifting the Veil: A Realist Critique of Sistema's Upwardly Mobile Path

    Science.gov (United States)

    Logan, Owen

    2016-01-01

    El Sistema sits somewhere between a social project and a classical music initiative. However, its promise of delivering upward mobility has not been sufficiently examined as a structural phenomenon which dovetails with critical policy issues in taxation, educational provision, human rights, and welfare. This article argues that Sistema-style…

  3. DIMENSIONAMIENTO DE UN SISTEMA DE ENERGÍA TERMOSOLAR MEDIANTE EL USO DE UN MODELO

    Directory of Open Access Journals (Sweden)

    Arturo Daniel Alarcón Rodríguez

    2004-01-01

    Full Text Available En el presente artículo se expone el método de dimensionamiento de sistemas termosolares mediante el uso de un modelo matemático. Este método es comúnmente usado debido que es simple, flexible pero a la vez muy potente. La simulación del sistema termosolar se realiza en base a un modelo matemático que describe los fenómenos térmicos que ocurren mediante un conjunto de ecuaciones diferenciales. Los parámetros que determinan el modelo son coeficientes de intercambio de calor entre los elementos del sistema, parámetros que representan las características de los componentes del sistema termosolar y parámetros que representan las condiciones en las que trabajará el sistema. Estos parámetros se determinan en base a recomendaciones de bibliografía, observaciones, mediciones de campo y correlaciones adecuadas. El uso de un modelo para el dimensionamiento de un sistema termosolar resulta una herramienta muy útil, ya que se adapta a distintas configuraciones de sistemas termosolares. Permite asimismo, tener una idea bastante aproximada del comportamiento del sistema termosolar en distintas condiciones de uso, la que sólo podría obtenerse a través de experimentos físicos complicados y por ende costosos.

  4. Malformaciones del sistema nervioso central en una familia

    Directory of Open Access Journals (Sweden)

    Elisa Dyce Gordon

    1998-06-01

    Full Text Available Se reporta la aparición de 3 malformaciones del sistema nervioso central (2 defectos del tubo neural y una hidrocefalia en la progenie de un matrimonio entre primos y se establece una posible relación entre la aparición de estos defectos y las herencias multifactorial y autosómica recesiva. Se recomienda evitar el matrimonio entre parientes, así como ofrecer asesoramiento genético y diagnóstico prenatal a todas las personas que han procreado un hijo con malformaciones del sistema nervioso centralIt is reported the appearance of 3 malformations of the central nervous system (2 defects of the neural tube and 1 hydrocephaly in the offspring of a marriage between cousins. A possible relationship between appearance of these defects and the multifactorial and recessive autosomal inheritances is established. It is recommended to avoid marriage between relatives, as well as to offer genetic counseling and prenatal diagnosis to all those persons who had procreated a child with malformations of the CNS

  5. Linfoma primario del sistema nervioso central en un paciente inmunocompetente

    Directory of Open Access Journals (Sweden)

    José Málaga-Zenteno

    2012-07-01

    Full Text Available El linfoma primario del sistema nervioso central (LPSNC constituye el 2% de los linfomas extranodales y entre 0,3% y 1,5% de todas las neoplasias intracraneales en pacientes inmunocompetentes, siendo más frecuente a partir de los 60 años. Reportamos el caso de un paciente varón de 76 años, sin antecedentes médicos de importancia, que inició su enfermedad con inestabilidad en la marcha, dificultad para mover el hemicuerpo izquierdo, a predominio braquial, cefalea holocraneal y mareos. Ingresó a emergencia despierto, parcialmente desorientado, Glasgow 14 y ptosis palpebral derecha; además, hemiparesia e hipoestesia izquierda, a predominio crural. Por tomografía computarizada cerebral se evidenció imagen captadora de contraste en región frontoparietal derecha, intra y extra craneal, edema cerebral y desplazamiento de la línea media. Se realizó craneotomía y biopsia del tumor, diagnosticándose linfoma difuso de células grandes B del sistema nervioso central. Fue VIH negativo. Se descartó otro tumor primario y metástasis. Recibió esquema CHOD/BVAM y radioterapia. Evolucionó favorablemente. Según resonancia magnética cerebral postratamiento, desapareció el tumor.

  6. Análisis de Estabilidad de Sistemas Borrosos

    Directory of Open Access Journals (Sweden)

    Basil M. Al-Hadithi

    2007-04-01

    Full Text Available Resumen: En este trabajo se revisa el estado del arte sobre estabilidad de sistemas borrosos, poniéndose de manifiesto las dificultades para su análisis, debido a la característica falta de linealidad de los mismos. Se revisan los estudios basados en el criterio del círculo, las técnicas para calcular Índices de estabilidad, así como técnicas basadas en aplicación del teorema de estabilidad de Lyapunov, que permite utilizar métodos númericos de búsqueda de soluciones. Además, se revisan los trabajos de estabilidad mediante el uso del modelo borroso de Takagi-Sugeno (T-S, el enfoque de las Desigualdades Matriciales Lineales (LMI, que ha tenido un interés creciente en los últimos años, así como otra línea de investigación basada en estabilidad energética. Palabras clave: Sistemas borrosos, Estabilidad global y asintótica, Modelo borroso afín de Takagi-Sugeno

  7. MODELO INTERACTIVO FUNCIONAL DEL SISTEMA DE TELEVISIÓN

    Directory of Open Access Journals (Sweden)

    Cesar Augusto Ramirez Corzo

    2011-01-01

    Full Text Available El siguiente artículo presenta el modelo comunicativo interactivo mediante el cual se describe, se analiza y se demuestra la incapacidad del sistema de televisión en Colombia para garantizar la protección de los menores de edad frente a los contenidos televisivos, destacándose tres subsistemas, el industrial, el humano y el legal. El modelo permite visualizar cómo funcionan y se integran los actores, cuáles son su propósitos, y el impacto que generan en el público receptor. El marco teórico se centra en el Interaccionismo Simbólico y en el estructural funcionalismo, para demostrar como el menor de edad queda totalmente expuesto a unos intereses económicos de la industria, sin suficiente protección del sistema legal, produciendo graves consecuencias en su dimensión espiritual, personal y social. El modelo comunicativo interactivo se convierte en un referente de análisis para investigaciones sociales que contemplen la influencia de los contenidos culturales en el individuo y en la sociedad.

  8. Sistema cenagoso de ayapel como posible sitio Ramsar en Colombia

    Directory of Open Access Journals (Sweden)

    Tatiana Puerta Quintana

    2016-01-01

    Full Text Available Colombia, al ser parte de la Convención internacional de humedales Ramsar, tiene como tarea la gestión de los humedales del país, incluyendo aquellos ecosistemas estratégicos que cumplan con uno o más criterios según los lineamientos dispuestos por la Convención. Este artículo describe el procedimiento a seguir para postular el sistema cenagoso de Ayapel como posible sitio Ramsar en Colombia. Se consolidaron los trabajos de investigación para demostrar que el sistema cenagoso de Ayapel cumple con varios criterios establecidos por la Convención. Como resultado relevante se obtuvo el insumo base para el proceso de postulación en la Convención (Ficha Ramsar. Este es el primer paso de un proyecto marco encaminado a la gestión y conservación de un ecosistema que sufre perturbaciones y transformaciones por actividades antrópicas, del cual depende en gran medida una población humana, de fauna y flora vulnerable, asociada a la dinámica de funcionamiento y servicios ecosistémicos del humedal. Además, se muestra el proceso de socialización de la Ficha Ramsar con la comunidad directamente involucrada, la cual acoge el proyecto de manera positiva.

  9. La organización empresarial como sistema adaptativo complejo

    Directory of Open Access Journals (Sweden)

    Luz Esperanza Bohórquez Arévalo

    2013-01-01

    Full Text Available El presente documento busca identificar las características de los sistemas adaptativos complejos (CAS, por su sigla en inglés Complexity Adaptative Systems , así como capturar las implicaciones de su aplica- ción en el estudio de las organizaciones. Para tal fin, se revisan resultados de investigación publicados en revistas académicas que han facilitado la introducción de las ciencias de la complejidad en la adminis- tración, al igual que se exploran los resultados de investigación de grupos e institutos que trabajan en el tema. A partir de los estudios comparativos de las propuestas observadas se plantea que los CAS pueden comprenderse desde la emergencia, la auto-organización y la evolución. La comprensión de la organiza- ción desde esta perspectiva genera líneas de investigación en liderazgo, estrategia, toma de decisiones y construcción de actuación conjunta; todo lo anterior en sistemas complejos.

  10. La organización empresarial como sistema adaptativo complejo

    Directory of Open Access Journals (Sweden)

    Luz Esperanza Bohórquez Arévalo

    2013-04-01

    Full Text Available El presente documento busca identificar las características de los sistemas adaptativos complejos (CAS, por su sigla en inglés Complexity Adaptative Systems, así como capturar las implicaciones de su aplicación en el estudio de las organizaciones. Para tal fin, se revisan resultados de investigación publicados en revistas académicas que han facilitado la introducción de las ciencias de la complejidad en la administración, al igual que se exploran los resultados de investigación de grupos e institutos que trabajan en el tema. A partir de los estudios comparativos de las propuestas observadas se plantea que los CAS pueden comprenderse desde la emergencia, la auto-organización y la evolución. La comprensión de la organización desde esta perspectiva genera líneas de investigación en liderazgo, estrategia, toma de decisiones y construcción de actuación conjunta; todo lo anterior en sistemas complejos.

  11. Lighting systems control; El control en los sistemas de iluminacion

    Energy Technology Data Exchange (ETDEWEB)

    Arriaga Alpizar, Eric [Instituto Politecnico Nacional (Mexico)]. E-mail: eric_aa@hotmail.com

    2006-04-15

    In any workplace the illumination systems are very important, nevertheless, it is essential to control them in order to save energy. This article presents the six following control strategies for the efficient electric power use: programming, lights only will be on when it is necessary; natural light use, illumination will be replaced by using sunlight; lumens level maintenance, average level illumination can be measured utilizing this method; adjustment, the lowest illumination average is required avoiding to affect the performance and adaptation-compensation, decreasing the system variation. In addition there are also control devices which will operate the system, such as automatic devices, presence sensors and photo sensors. [Spanish] Los sistemas de iluminacion son muy importantes en cualquier lugar de trabajo, no obstante, es necesario que tengan un control, para lograr ahorrar energia. Este articulo presenta seis estrategias de control para el uso eficiente de la energia electrica: la programacion, con esta medida solo se encenderia la luz cuando sea necesario; uso de luz natural, asi se puede prescindir de la iluminacion pues la luz solar cumple con su funcion; mantenimiento del nivel de lumenes, de esta manera se mantiene un nivel promedio de iluminacion; ajuste, se ocupa el nivel de luz mas bajo posible sin afectar el rendimiento y adaptacion-compensacion, reduce la variacion del sistema. Por otro lado tambien hay dispositivos de control, que haran el trabajo, tales como dispositivos automaticos, sensores de presencia y fotosensores.

  12. Dificultades actuales del sistema internacional de derechos humanos

    Directory of Open Access Journals (Sweden)

    José Augusto Lindgren-Alves

    2017-11-01

    Full Text Available Los derechos humanos, después de alzcanzar el punto culminante de popularidad en la Conferencia de Viena de 1993, entraron en uma fase de descrédito, que aún perdura. Eso ocurrió por varios motivos, algunos de los cuales están al interior del propio sistema estabelecido para promocionarlos. Los factores principales advienen de la contradicción entre la aserción verbal de los derechos fundamentales de todos y el “consenso” neoliberal simultaneamente impuesto a la esfera económica. Otros son inherentes al activismo fragmentario, sin noción de causa y efecto, desvinculado de la realidad en vuelta. Los problemas de segundo tipo son corregibles si para eso hubiese coherencia y destemor de críticas en las atitudes de los actores influyentes. El texto examina los componientes de ese sistema internacional e identifica las razones de su presente desvaluación.

  13. Producción lechera en un sistema silvopastoril mejorado

    Directory of Open Access Journals (Sweden)

    José R. Casermeiro

    2008-01-01

    Full Text Available Se evaluó la producción lechera en un pastizal natural bajo bosque nativo mejorado para demostrar la factibilidad de generar sistemas de producción lechera sustentables para pequeños productores del norte de la Provincia de Entre Ríos, en áreas consideradas no aptas. Se estimó la composición y condición del pastizal y bosque nativo, producción y calidad del pastizal natural y leche y se caracterizó el suelo en sus aspectos físico-químicos, nutricionales y químicos. La subdivisión de lotes, los descansos del pastizal, la mayor disponibilidad de forraje, la rotación de los animales, la carga animal adecuada, la limpieza del bosque y la intersiembra, son herramientas claves para la sustentabilidad y rentabilidad. El bosque nativo constituye un ambiente confortable para los animales, las especies forrajeras y el suelo. Los pequeños productores pueden producir leche en un sistema silvopastoril mejorado sin asumir costos de implantación y mantenimiento que implica la producción lechera con pasturas cultivadas

  14. Histoplasmosis del sistema nervioso central en un paciente inmunocompetente

    Directory of Open Access Journals (Sweden)

    Natalia Osorio

    2014-12-01

    Full Text Available La histoplasmosis es una afección polifacética producida por el hongo dimorfo Histoplasma capsulatum, cuyas esporas son inhaladas y llegan al pulmón, órgano primario de infección. La forma meníngea, considerada como una de las manifestaciones más graves de esta micosis, suele presentarse en individuos con alteraciones en la inmunidad celular: pacientes con síndrome de inmunodeficiencia humana adquirida, con lupus eritematoso sistémico o con trasplante de órgano sólido, así como en lactantes, debido a su inmadurez inmunológica. La forma de presentación más usual es de resolución espontánea y se observa en individuos inmunocompetentes que se han expuesto a altas concentraciones de conidias y fragmentos miceliares del hongo. En estas personas, la afección se manifiesta por trastornos pulmonares y por la posterior diseminación a otros órganos y sistemas. Se presenta un caso de histoplasmosis del sistema nervioso central en un niño inmunocompetente.

  15. SISTEMA DE CREENCIAS ACERCA DE LA MATEMÁTICA

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    Oswaldo Jesús Martínez Padrón

    2014-01-01

    Full Text Available Este artículo reporta una investigación cuyo objetivo fue comprender las acciones observadas tanto en estudiantes que se forman para enseñar Matemática, como en sus docentes, en función del sistema de creen cias acerca de la enseñanza de la Matemática, su aprendizaje o su evaluación. Dichos estudiantes cursaban la carrera de Educación Integral en una Universidad Pedagógica, que sirvió de contexto para validar empíricamente un conjunto de postulados teóricos a través de un trabajo de campo de carácter descriptivo, concretado en un estudio etnográfico. Con la información obtenida mediante observaciones y entrevistas, se interpretaron las acciones y se confirmó, entre otros aspectos, que el sistema de creencias d e estos actores impacta en sus actuaciones, destacando que: (a los docentes continúan atados al modelo concepto - ejemplo - ejercicios, sin invitar a los estudiantes a resolver problemas, ni solicitarles el uso de sus conocimientos previos, y (b los estudian tes presentan actitudes desfavorables hacia la Matemática y también aversión, por pensarla aburrida, compleja y difícil de entender.

  16. Sistema de salud de Brasil The health system of Brazil

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    Víctor Becerril Montekio

    2011-01-01

    Full Text Available En este trabajo se describe el sistema de salud de Brasil, que está compuesto por un sector público que cubre alrededor de 75% de la población y un creciente sector privado que ofrece atención a la salud al restante 25% de los brasileños. El sector público está constituido por el Sistema Único de Salud (SUS y su financiamiento proviene de impuestos generales y contribuciones sociales recaudadas por los tres niveles de gobierno (federal, estatal y municipal. El SUS presta servicios de manera descentralizada a través de sus redes de clínicas, hospitales y otro tipo de instalaciones, y a través de contratos con establecimientos privados. El SUS es además responsable de la coordinación del sector público. El sector privado está conformado por un sistema de esquemas de aseguramiento conocido como Salud Suplementaria financiado con recursos de las empresas y/o las familias: la medicina de grupo (empresas y familias, las cooperativas médicas, los llamados Planes Autoadministrados (empresas y los planes de seguros de salud individuales. También existen consultorios, hospitales, clínicas y laboratorios privados que funcionan sobre la base de pagos de bolsillo, que utilizan sobre todo la población de mayores ingresos. En este trabajo se analizan los recursos con los que cuenta el sistema, las actividades de rectoría que se desarrollan y las innovaciones más recientemente implantadas, incluyendo el Programa de Salud de la Familia y el Programa Más Salud.This paper describes the Brazilian health system, which includes a public sector covering almost 75% of the population and an expanding private sector offering health services to the rest of the population. The public sector is organized around the Sistema Único de Saúde (SUS and it is financed with general taxes and social contributions collected by the three levels of government (federal, state and municipal. SUS provides health care through a decentralized network of clinics

  17. Sistema de salud de Chile The health system of Chile

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    Víctor Becerril-Montekio

    2011-01-01

    Full Text Available En este trabajo se describe el sistema de salud de Chile, incluyendo su estructura, financiamiento, beneficiarios y recursos físicos, materiales y humanos de los que dispone. Este sistema está compuesto por dos sectores, público y privado. El sector público está formado por todos los organismos que constituyen el Sistema Nacional de Servicios de Salud y cubre aproximadamente a 70% de la población, incluyendo a los pobres del campo y las ciudades, la clase media baja y los jubilados, así como los profesionales y técnicos. El sector privado cubre aproximadamente a 17.5% de la población perteneciente a los grupos sociales de mayores ingresos. Un pequeño sector de la población, perteneciente a la clase alta, realiza pagos directos de bolsillo a proveedores privados de servicios de atención a la salud. Alrededor de 10% de la población está cubierta por otras agencias públicas, fundamentalmente los Servicios de Salud de las Fuerzas Armadas. Recientemente el sistema se reformó creando el Régimen General de Garantías en Salud, que establece un Sistema Universal con Garantías Explícitas que se tradujo, en 2005, en el Plan de Acceso Universal con Garantías Explícitas (AUGE, que garantiza el acceso oportuno a servicios de calidad para 56 problemas de salud, incluyendo cáncer en niños, cáncer de mama, trastornos isquémicos del corazón, VIH/SIDA y diabetes.This paper describes the Chilean health system, including its structure, financing, beneficiaries, and its physical, material and human resources. This system has two sectors, public and private. The public sector comprises all the organisms that constitute the National System of Health Services, which covers 70% of the population, including the rural and urban poor, the low middle-class, the retired, and the self-employed professionals and technicians.The private sector covers 17.5% of the population, mostly the upper middle-class and the high-income population. A small

  18. Ajustes a la arquitectura del sistema general de salud de Colombia: una propuesta

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    Dov Chernichovsky

    2015-04-01

    Full Text Available El modelo de competencia regulada establecido por la Ley 100 de 1993 para el funcionamiento del sistema de salud en Colombia presenta fallas de mercado y fallas de gobierno. A la luz del modelo conceptual del «Paradigma Emergente», este artículo presenta una propuesta de adaptación de la arquitectura del sistema de salud de Colombia a la arquitectura de sistemas internacionales considerados exitosos, identificando instituciones, roles y competencias. Dado lo anterior, las recomendaciones más importantes son: impulsar el sistema dual de competencia regulada en zonas densamente pobladas y monopolios en zonas sin densidad geográfica y regionalizar el sistema. También se sugiere la adopción de instrumentos de regulación de la oferta como el certificado de necesidad.

  19. Expert system based on cases for a diagnosis system in real time; Sistema experto basado en casos para un sistema de diagnostico en tiempo real

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    Espinosa R, Alfredo; Quintero R, Agustin; Zambrano D, S Venecia [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)

    2006-07-01

    This article presents the development of an Expert System based in the Reasoning Based on Cases methodology. Such system was performed with the purpose of creating an information system in charge of supervising and diagnosing the status of the main equipment of fossil fuel power plants for electricity generation. Here is presented the reasons why this methodology was used for the expert system and why Induce-It -the specialized tool that implements it- was also chosen, as well as the analysis made for the disposition of the operative architecture of the Expert System, the very development of this software architecture and, finally, the validation of the correct operation of this system by means of a simulator that simultaneously puts to the test the error handling of the Expert System. [Spanish] Este articulo presenta el procedimiento que siguio el desarrollo de un Sistema Experto asentado en la metodologia de Razonamiento Basado en Casos; realizado con el fin de crear un sistema de informacion encargado de supervisar y diagnosticar el estado de los equipos principales de centrales de generacion termoelectrica. Se expone justificadamente la seleccion de la metodologia del sistema experto y de la herramienta especializada que lo implementa (Induce-It), asi como el analisis realizado para la disposicion de la arquitectura operativa del Sistema Experto, el desarrollo mismo de esta arquitectura del software y, finalmente, la validacion del correcto funcionamiento de este sistema mediante un simulador que a la vez pone a prueba el manejo de errores del Sistema Experto.

  20. Estudio comparativo entre metodologías para el diseño de Sistemas Bonus-Malus.

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    Núñez del Prado, José Antonio

    2004-01-01

    Full Text Available El objetivo de este trabajo es mostrar un método para hacer comparaciones entre Sistemas Bonus Malus. Se comparan Sistemas Bonus Malus obtenidos mediante la metodología GPBM, basada en Programación por Metas y Sistemas Bonus Malus obtenidos mediante la metodología clásica, conocida como Escala de Bayes.

  1. Co-generation system with integrated functions of active filter; Sistema de cogeneracion con funciones de filtro activo integradas

    Energy Technology Data Exchange (ETDEWEB)

    Jimenez Grajales, Humberto Raul

    2002-07-15

    adaptive interference. This technique maintains the system in the best functional state, by means of continuous self adjustment facing changes in the operation parameters, generating important and satisfactory results. In addition, simultaneously the PVS uses, in the pursuit of the point of maximum power, the technique of {sup d}isturb and observe{sup ,} which offers a high performance in the injection of active power. [Spanish] Sin duda, la energia electrica es la forma de energia mas ampliamente usada en las actividades economicas y sociales de nuestro mundo moderno. Por ello, se puede decir que las companias generadoras y distribuidoras de electricidad asumen dos retos de relevante preocupacion actual. Uno es generar con mayor eficiencia, menor costo y con un minimo impacto sobre el medio ambiente. El segundo es cumplir con la calidad del suministro, que ha llegado a ser un punto importante tanto para los usuarios como para las companias que distribuyen la energia electrica, y que se refleja en una distribucion y un consumo de energia mas eficiente. El trabajo de tesis que se presenta, describe un sistema fotovoltaico (SFV) interconectado a la red electrica que ayuda a superar los retos citados. Este SFV tiene la funcion de recibir la energia solar entregada por un arreglo de celdas FV; operar en el punto maximo de potencia de las celdas (para mayor aprovechamiento de la energia captada); acondicionarla a una senal alterna, dentro de los parametros tecnicos normativos para la interconexion electrica (establecidos por la compania; Comision Federal de Electricidad en Mexico); e inyectarla a la red electrica con el proposito de compensar potencia reactiva (filtrado activo) demandada por cargas lineales y no lineales, reduciendo la contaminacion de la red electrica; ademas de compensar potencia activa en los picos de demanda de energia electrica maxima. El sistema funciona como filtro activo durante todo el dia, lo que permite que la capacidad de potencia instalada por el

  2. O sistema alexandrino e o sistema epicurista na filosofia da história do jovem Marx

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    Júlia Lemos Vieira

    2017-10-01

    Full Text Available Nos Cadernos preparatórios de sua Tese de Doutorado intitulada Diferença entre as filosofias de Demócrito e Epicuro, esboçados em 1839, Marx se contrapôs a um conteúdo teológico em Hegel através de uma crítica à tendência tradicional da filosofia em teologizar-se dada numa revisão do ocaso da filosofia grega em dois sistemas opostos. O primeiro teria sido o misticismo da filosofia alexandrina; o segundo, o atomismo epicurista que desenvolveu uma dialética peculiar. Sugerindo uma filosofia da história alternativa à de Hegel, Marx pretendia indicar que a democracia não sofreu ocaso na Grécia por conta do desenvolvimento da razão genuinamente filosófica, e sim por conta da vitória da razão filosófica tergiversada em teologia. Era preciso, neste sentido, resgatar a filosofia do sistema epicurista.

  3. La base demográfica de los sistemas socioculturales

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    Faus Pujol, María Carmen

    2012-12-01

    Full Text Available In Social and Cultural Geography, understood as the «study of the relationship between humans and their environment» and as «science of human settlers», demographic systems are the foundation of sociocultural systems. Demographic systems are built at the expense of the population that lives, so unevenly spread out, on the surface of the earth at any given time and whose components live together and group in order to satisfy their needs. Humanity as a whole is differentiated by the number of individuals that make up the different groups, their characteristics and the dynamics of the processes of internal growth. These factors allow one to differentiate the different demographic systems using a scale of magnitudes in relation to the number of individuals they contain, the nature of their needs and their territorial sphere of influence. Among other things, their effectiveness and stability depend on the sociocultural level of the members of the group. When the effectiveness of a demographic system diminishes, it enters a stage of degradation which concludes in the explicit manifestation of the deficit. The demographic foundation of sociocultural systems is made up of microsystems whose stability usually depends on the living conditions of the population.

    En Geografía Social y Cultural, entendida como «estudio de las relaciones entre el hombre y el medio» y como «ciencia del hombre habitante», los sistemas demográficos constituyen el fundamento de los sistemas socioculturales. Los sistemas demográficos se construyen a expensas de la población que en cada momento vive en las diferentes porciones de la superficie terrestre, muy irregularmente repartida sobre el planeta, y cuyos componentes conviven y se agrupan para satisfacer sus necesidades. El conjunto de la Humanidad se presenta diferenciado por el número de individuos que los integran, por las características de éstos y por la dinámica de los procesos de crecimiento

  4. La Liberalización (extrema del Sistema de Protección Social Mexicano

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    Ilán Bizberg

    2016-12-01

    Full Text Available Resumen En este trabajo analizamos la transformación del sistema de protección social mexicano del corporativismo al asistencialismo, de un sistema construido sobre la base de un intercambio corporativo con las organizaciones sociales que eran la fundamento del PRI a otro en el que los pobres se han convertido en la fuente del poder político. Por otra parte, se pasó de un sistema de seguridad social basado en derechos adquiridos a otro en el que el sistema de seguridad social depende cada vez más de decisiones de política pública. Está dividido en cuatro partes: en la primera, hacemos un breve recuento histórico del sistema corporativo; en la segunda, analizamos las reformas al sistema de pensiones; en la tercera, las reformas al sistema de asistencia a los pobres; y en la cuarta, las reformas al sistema de salud. Palabras clave: Sistema de seguridad social; corporativismo; asistencia; salud, pensiones, México  *** The (extreme Liberalization of the Mexican Social Protection System. Abstract In this paper we analyze the transformation of the Mexican social protection system from corporatism to assistance, from a system built on the basis of a corporatist exchange with the social organizations that were the foundation of the PRI to another in which the poor have become the source of political power. On the other hand, it has moved from a social security system based on acquired rights to another one in which the social security system increasingly depends on public policy decisions. It is divided into four parts: in the first, we make a brief historical account of the corporatist social protection system; In the second, we analyze the reforms to the pension system; In the third, the shift form corporatism to assistance to the poor; And in the fourth, the reforms of the health system. Keywords: Social security system, corporatism, assistance; health, pensions, Mexico.  *** A Liberalização (extremo do Sistema de Proteção Social

  5. Controlador empotrado en FPGA para Sistema Inteligente de Transporte

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    Alejandro José Cabrera Sarmiento

    2011-11-01

    Full Text Available 1024x768 Normal 0 21 false false false ES X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} En el presente trabajo se expone la concepción, desarrollo e implementación de un controlador empotrado en un FPGA de Xilinx para ser utilizado en un Sistema Inteligente de Transporte (SIT. La estructura hardware del controlador está basada en la utilización de diversos módulos de propiedad intelectual del sistema de procesamiento MicroBlaze y el soporte de software está basado en la utilización del sistema operativo Petalinux. El controlador empotrado dispone de interfaces Ethernet, USB, UART, SPI e I2C para la comunicación con los diferentes niveles jerárquicos del SIT. Ha sido implementado sobre una placa de desarrollo basada en un FPGA Spartan3E de 1.200 k compuertas, ocupando un 59% de sus recursos configurables. El resto de los recursos disponibles en el FPGA permite, además de la posible actualización del controlador, la implementación hardware de algoritmos que requieren una alta velocidad de procesamiento.

  6. Sistema de salud de Argentina The health system of Argentina

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    Mariana Belló

    2011-01-01

    Full Text Available En este artículo se describe el sistema de salud de Argentina, que está compuesto por tres sectores: público, de seguridad social y privado. El sector público está integrado por los ministerios nacional y provincial, y la red de hospitales y centros de salud públicos que prestan atención gratuita a toda persona que lo demande, fundamentalmente a personas sin seguridad social y sin capacidad de pago. Se financia con recursos fiscales y recibe pagos ocasionales de parte del sistema de seguridad social cuando atiende a sus afiliados. El sector del seguro social obligatorio está organizado en torno a las Obras Sociales (OS, que aseguran y prestan servicios a los trabajadores y sus familias. La mayoría de las OS operan a través de contratos con prestadores privados y se financian con contribuciones de los trabajadores y patronales. El sector privado está conformado por profesionales de la salud y establecimientos que atienden a demandantes individuales, a los beneficiarios de las OS y de los seguros privados. Este sector también incluye entidades de seguro voluntario llamadas Empresas de Medicina Prepaga que se financian sobre todo con primas que pagan las familias y/o las empresas. En este trabajo también se describen las innovaciones recientes en el sistema de salud, incluyendo el Programa Remediar.This paper describes the health system of Argentina.This system has three sectors: public, social security and private.The public sector includes the national and provincial ministries as well as the network of public hospitals and primary health care units which provide care to the poor and uninsured population. This sector is financed with taxes and payments made by social security beneficiaries that use public health care facilities. The social security sector or Obras Sociales (OS covers all workers of the formal economy and their families. Most OS operate through contracts with private providers and are financed with payroll

  7. Biomarcadores en enfermedades relacionadas con el sistema nervioso

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    Elso Manuel Cruz Cruz

    2014-12-01

    Full Text Available El estudio de biomarcadores, o marcadores biológicos para el diagnóstico y seguimiento al tratamiento de enfermedades, constituye referente frecuente en la literatura científica biomédica actual. Se realizó una revisión bibliográfica empleando los recursos disponibles en la red Infomed, específicamente Ebsco, The Cochrane Librery, PubMed, Hinari y SciELO, a través de los cuales se accedieron a las bases de datos: MEDLINE, Academic Search Premier, MedicLatina y Scopus. Se recopilaron varios artículos científicos, donde se hace referencia al empleo de biomarcadores en el diagnóstico de enfermedades relacionadas con el sistema nervioso. El factor de crecimiento endotelial, la inmunoglobulina G (IgG y el sistema arginina - óxido nítrico - citrulina, se refieren como indicadores prematuros de la hemorragia subaracnoidea. La disminución del amiloide beta y el aumento de las concentraciones de proteína tau en el líquido cefalorraquídeo, se cuentan entre los biomarcadores más prometedores e informativos que indiquen precozmente el Alzheimer y otras enfermedades neurodegenerativas. Estos mismos marcadores se comportan de manera contraria a nivel plasmático. La copeptina es un biomarcador fácilmente medible, que refleja la situación de estrés a la que está sometido el organismo y se propone su uso en el diagnóstico del síndrome de Burnout; además, niveles aumentados de α-amilasa y cromogranina A (CgA salivales pueden también ser utilizadas como indicadores de este síndrome. Se logró recoger en este artículo un compendio de ejemplos de marcadores biológicos, utilizados en el diagnóstico de enfermedades relacionadas de alguna forma con el sistema nervioso

  8. Salud: un sistema complejo adaptativo Health: an adaptive complex system

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    Luis Fernando Toro-Palacio

    2012-02-01

    Full Text Available Este artículo destaca la enorme distancia existente entre el pensamiento complejo de índole intelectual, difundido en nuestro medio, y el pensamiento complejo de índole experimental, que ha permitido lograr los desarrollos científico-tecnológicos que han cambiado radicalmente el mundo. Invita a considerar como sistemas complejos adaptativos entidades tales como la vida, el ser humano, la sociedad global y todo aquello que se llama salud, para lo cual resulta prioritario adoptar un enfoque diferente que amplíe su conocimiento. Al reconocer esta racionalidad, se sustentan las principales características y propiedades emergentes de la salud como sistema complejo adaptativo, siguiendo un modelo de prestación de cuidados y servicios. Finalmente, se plantean algunas preguntas de investigación pertinentes desde esta perspectiva, y se expresan una serie de apreciaciones que se espera sirvan para comprender todo lo que como individuos y como especie hemos llegado a ser. Se propone en este trabajo que la salud y la prestación de servicios de asistencia sanitaria se consideren como sistemas complejos adaptativos.This article points out the enormous gap that exists between complex thinking of an intellectual nature currently present in our environment, and complex experimental thinking that has facilitated the scientific and technological advances that have radically changed the world. The article suggests that life, human beings, global society, and all that constitutes health be considered as adaptive complex systems. This idea, in turn, prioritizes the adoption of a different approach that seeks to expand understanding. When this rationale is recognized, the principal characteristics and emerging properties of health as an adaptive complex system are sustained, following a care and services delivery model. Finally, some pertinent questions from this perspective are put forward in terms of research, and a series of appraisals are expressed that

  9. TERRORISMO, SALUD PÚBLICA Y SISTEMAS SANITARIOS

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    Pedro Arcos González

    2009-01-01

    Full Text Available El terrorismo es hoy un problema de distribución global e interés creciente para la salud pública internacional. La violencia que comporta afecta a la salud pública y los servicios sanitarios de manera importante y en diferentes ámbitos, entre ellos, aumenta la mortalidad, morbilidad y discapacidad, genera un contexto de miedo y ansiedad que hace muy frecuentes los trastornos psicopatológicos, altera gravemente el funcionamiento de los servicios sanitarios y produce importantes daños sociales, políticos y económicos. Estos efectos son, además, especialmente intensos cuando el fenómeno se produce de manera crónica en una comunidad. El objetivo de este trabajo es examinar la relación entre terrorismo y salud pública, particularmente en cuanto a sus efectos sobre esta y sobre los servicios sanitarios, así como examinar los posibles marcos de abordaje del terrorismo como problema de salud pública, con especial referencia a la situación en España. Frente a este problema, tanto los sistemas de salud pública como los servicios sanitarios, deberían adaptar sus enfoques y funcionamiento especialmente en seis ámbitos prioritarios relacionados con: (1 La coordinación entre las diferentes agencias de respuesta sanitaria y no sanitaria; (2 El refuerzo de los sistemas de vigilancia epidemiológica; (3 La mejora de las capacidades de los laboratorios de salud pública y de los sistemas de respuesta sanitaria a tipos específicos de terrorismo como son el terrorismo químico o biológico; (3 Los servicios de salud mental; (4 La planificación y coordinación de la respuesta de emergencia de los servicios sanitarios; (5 Las relaciones con la población y los medios de comunicación y, finalmente; (6 Una mayor transparencia en la difusión de la información y un mayor grado de análisis de las acciones sanitarias llevadas a cabo en el ámbito de la respuesta de emergencia.

  10. Sistemas de control de vibraciones en estructuras de gran altura

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    Montanaro, Maria Inés

    2002-02-01

    Full Text Available The increasing of big cities with a great population density, flows to the multiplication of light and thin towers in very high buildings, with an small natural damping. So, those buildings are exposed to the wind oscillations and to the earthquakes. Those oscillations could produce damages in the structure, affect its functionality and/or to be a cause of discomfort. In order to reduce the structural risk of excessive deformations or accelerations, we want to bring up here a solution lied with the dissipation of vibrational energy in the structural dampers. The objective of this work is to bring a c1ear and complete c1assification and description of all isolation and vibration controls known till the moment and their main application' fields. The definition of those systems is completed with graphics, characteristics and structures examples. This c1assification will allow a real interpretation of advantages and disadvantages of all isolation and vibration controls concerning structural applications.El aumento de la densidad poblacional y de las grandes urbes ha incrementado la necesidad de las torres de edificios de gran altura, las cuales tienden a ser muy livianas y delgadas, por lo tanto poseen un amortiguamiento natural muy pequeño, lo que los hace más propensos a las oscilaciones del viento y los sismos. Dichas oscilaciones pueden causar daño a la estructura, afectar su funcionalidad y/o causar incomodidad. Una solución planteada para reducir el riesgo estructural de experimentar deformaciones excesivas o aceleraciones es la disipación de energía vibracional en los amortiguadores estructurales. El objetivo de este trabajo es brindar una clasificación clara y completa de todos los sistemas de aislación y control de vibraciones existentes hasta al momento, además de una descripción de los mismos y sus principales campos de aplicación. La definición de los sistemas incluye gráficos, características y ejemplos de estructuras

  11. SISTEMA FLUVIAL E PLANEJAMENTO LOCAL NO SEMIÁRIDO

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    Jonas Otaviano Praça de Souza

    2012-01-01

    Full Text Available El presente estudio analiza un sistema fluvial semiárido y sus características físicas, centrándose en los procesos geomorfológicos y las formas resultantes, sino también en las relaciones con las actividades humanas, con el fin de utilizar dichos datos en la planificación local. La encuesta se llevó a cabo en el municipio de Belém do São Francisco, en Pernambuco, mesorregión del São Francisco en la cuenca del arroyo Mulungu, lugar expuesto a un clima semi-árido con lluvias de verano, y la cobertura del suelo con un predominio de la caatinga arbustiva abierta. Se tomó como procedimiento metodológico la cartografía geomorfológica a diferentes escalas y con diferentes énfasis, para evaluar la relación jerárquica entre los distintos compartimentos, sustratos geológicos y formas de uso de la tierra. De la información espacial obtenida en los distintos niveles de la cartografía detallada del sistema fluvial, se realizó una evaluación ambiental de la zona, teniendo como base la dinámica erosiva/deposicional a lo largo del canal y su relación con sus bancos. Se concluyó que las formas de acumulación en la llanura aluvial exhiben controles de origen antropogénico, vinculados a los tipos tradicionales de uso del suelo en la cuenca. Estos controles, como la construcción de represas a lo largo del canal, actúan cambiando a los procesos de creación de nuevas morfologías de depósito en el sistema fluvial, que a su vez comienzan a redefinir los tipos usos de la zona.

  12. Modelo decisional proactivo en sistemas ecológicos (modepec.

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    Pedro Tolón Estarelles

    2007-07-01

    Full Text Available Los sistemas económicos y sociales son de tipo ecológico, y se pueden enmarcar en modelos de sistemas complejos de tipo DYSES (Dynamic Social and Economic Systems. No son  explicables o predecibles satisfactoriamente  con modelos determinísticos, pues, en general obedecen a procesos evolutivos de carácter estocástico, respondiendo en su interacción con el medio al segundo principio de la termodinámica en el sentido de la irreversibilidad (falta de simetría temporal y aumento de la entropía. MODEPEC © es un modelo que postula una metodología que asegura  un proceso  de formulación de criterios  para mejorar continuamente la calidad en la toma  de decisiones en sistemas ecológico-social-económicos. Los conceptos esenciales del modelo se enuncian seguidamente: 1-. Existencia de una Ecuación de Estado (EE Un sistema ecológico tiene un  comportamiento dinámico posible de ser representado mediante una ecuación de estado en cualquier intervalo de tiempo dado. 2-. Estimación de una política óptima (PO Es posible estimar  un conjunto óptimo de decisiones (política óptima. 3-. Contrato sobre estado deseable  (CED En la comunidad  social y económica de tipo ecológico, es posible acordar un diseño de estado ideal deseable, constituido a partir de un contrato sustentable a largo plazo entre las partes. 4-. Genotipo Decisional Planificado (GDP Es posible establecer un proceso racional de toma de decisiones, en este caso, el Genotipo Decisional Planificado  (GDP; capaz de asegurar niveles crecientes de calidad en la determinación de criterios para evaluación y adopción de cursos de acción en pos del logro de un estado deseado (ED tomado como propósito o contrato. The economic and social systems are of ecological type, and they are possible to be framed in models of complex systems of type SDES (Social Dynamic and Economic Systems. They are not explicable or predictable satisfactorily with deterministic models, then

  13. EL SISTEMA FINANCIERO EN 1996 Y PERSPECTIVAS PARA 1997

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    Julio César Espinosa Rodríguez

    2011-09-01

    Full Text Available La evolución del sistema financiero en 1996 estuvo circunscripta dentro de la tendencia de los últimos cuatro años de una continua disminución de la tasa de crecimiento del PIB, comportamiento enmarcado dentro de una serie de desequilibrios financieros. Dado lo anterior, se implementaron políticas encaminadas a la restricción de los gastos en la economía, aspecto evidenciado en el incremento de los impuestos y en las elevadas tasas de interés, lo cual definió a 1996 como un año recesivo. Empero, a pesar de los problemas citados, se produjo un avance en la innovación de nuevos instrumentos financieros, aunado a una mayor participación de los inversionistas institucionales.

  14. Estudo do sistema ligninolitico do ascomiceto chrysonilia sitophila

    OpenAIRE

    Jaime Patricio Rodriguez Gutierrez

    1990-01-01

    Resumo: Neste trabalho, foi estudada a produção e caracterização as ligninases de Chrysonilia sitophila, a biodegradação de lignossulfonato e outros aspectos relacionados com o sistema ligninolítico deste ascomiceto. A produção de enzimas foi influenciada pela composição do substrato, sendo que materiais contendo lignina, como madeira de pinus, bagaço de cana e casca de arroz, induziram a uma maior produção de fenol-oxidases e de H2O2. Alta concentração de nitrogênio (76mM/l) e cultura não ox...

  15. Sistema de prototipos para control numérico

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    Abdón Sánchez Sossa

    1992-05-01

    Full Text Available La mejor utilización de equipos de alta tecnología implica el contar con el adecuado entrenamiento. La conformaci6n del Grupo de Control Numérico Computarizado de Ingenierfa de Sistemas en 1987, pionero, al permitir canalizar y consolidar en tres cursos electivos interdisciplinarios una respuesta a las inquietudes académico-docentes de apoyo al sector manufacturero. El desarrollo de varios programas, cuya complejidad ha venido creciendo con el tiempo, permitió explorar la aplicabilidad de los conceptos presentados en clase y sirvió de vinculo con la realidad Nacional de manera activa y comprometida, con el desarrollo del país. Los programas descritos en este trabajo son el resultado de muchas horas de labor y realizados contando con un exiguo presupuesto.

  16. Calagem na superfície em sistema plantio direto

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    E. F. Caires

    2000-03-01

    Full Text Available Existem informações conflitantes a respeito da eficiência da aplicação superficial de calcário em sistema plantio direto, particularmente na correção da acidez do subsolo, e de critérios de recomendação de calagem para tal sistema de cultivo. Com o objetivo de avaliar a correção da acidez do solo e a produção de grãos de culturas em rotação no sistema plantio direto, considerando a aplicação de calcário na superfície, foi realizado um experimento em um Latossolo Vermelho-Escuro distrófico textura média, em Ponta Grossa (PR, no período de 1993 a 1998. Os tratamentos, dispostos em blocos completos ao acaso com três repetições, constaram de quatro doses de calcário dolomítico: 0, 2, 4 e 6 t ha-1, calculadas para elevar a saturação por bases do solo a 50, 70 e 90%. O calcário foi aplicado em julho de 1993, a lanço, na superfície do solo. Foram cultivados, na seqüência, soja (1993/94, milho (1994/95, soja (1995/96, trigo (1996, soja (1996/97, triticale (1997 e soja (1997/98. A aplicação de calcário na superfície aumentou significativamente o pH, os teores de Ca + Mg trocáveis e a saturação por bases e reduziu significativamente os teores de H + Al do solo, até a profundidade de 60 cm. Estimou-se que a máxima eficiência técnica (MET e a econômica (MEE ocorreriam, respectivamente, com as doses de 3,8 e 3,3 t ha-1 de calcário. A dose de calcário para MEE foi a indicada pelo método da elevação da saturação por bases do solo a 65%, para amostra coletada na profundidade de 0-20 cm, mostrando ser este critério adequado para recomendação de calagem na superfície em sistema plantio direto. Todavia, a calagem na superfície deve ser recomendada somente para solo com pH (CaCl2 inferior a 5,6 ou saturação por bases inferior a 65%, na camada de 0-5 cm.

  17. Implantação do sistema APPCC para sorvetes

    OpenAIRE

    Borszcz, Valeria

    2002-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia de Alimentos. Este trabalho, objetiva implantar o sistema APPCC para a produção de sorvete, na empresa Kimyto Industrial Ltda localizada na cidade de Taió, Santa Catarina. Visa-se não apenas a identificação e controle dos perigos químicos, físicos e microbiológicos, mas também dos parâmetros para a qualidade. Com auxílio da árvore decisória, para cada etapa de proces...

  18. Implementação de sistemas SAP em empresas de serviços públicos

    OpenAIRE

    Gonçalves, Elisabete Gabriela de Oliveira

    2012-01-01

    Dissertação de mestrado em Engenharia e Gestão de Sistemas A utilização de sistemas Enterprise Resource Planning (ERP) para organizar a informação relevante de uma organização é cada vez mais frequente. Com este documento pretende-se explorar o percurso profissional na implementação de sistemas ERP, mais precisamente na implementação de sistemas SAP para empresas de serviços públicos, ou seja implementação de sistemas SAP IS-U. Apesar do foco principal do presente documento ...

  19. La teoría del granulado y su aplicación a sistemas moleculares complejos

    OpenAIRE

    Hijón de Miguel, Carmen

    2009-01-01

    El objetivo de esta tesis es el desarrollo de modelos de grano grueso para la simulación de sistemas moleculares complejos. Entendemos que un modelo de grano grueso es aquel que representa un sistema físico con menor número de grados de libertad que los que hay realmente en el sistema. Y por sistema molecular complejo entendemos aquel cuyos constituyentes son macromoléculas. El reto que plantea la simulación de los sistemas moleculares complejos es el desarrollo de modelos capaces de describi...

  20. El nuevo sistema de pensiones mejicano Comparación del rendimiento neto de las administradoras

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    Ignacio Contreras Rubio

    2000-01-01

    Full Text Available Con la reforma mejicana de julio de 1997 se culminaba un proceso de reformas iniciado en Chile en 1981 por el que los principales países latinoamericanos han optado por sistemas de pensiones basados en la capitalización individual, cediendo la gestión de los fondos destinados a financiar el retiro a entidades privadas que actúan bajo competencia. En el presente trabajo se plantea la formulación matemática del nuevo sistema mejicano de pensiones tras la reforma de julio de 1997, con el que se opta por un sistema de capitalización individual frente al sistema de reparto vigente hasta ese momento. El trabajo se estructura de la siguiente forma, en sucesivos apartados se describen los elementos determinantes del sistema, tales como aportaciones, comisiones y capitalización de los fondos, describiendo sus características así como su inclusión dentro de una fórmula recursiva que permita conocer el valor de los fondos acumulados en cada momento por el trabajador. Esta formulación será utilizada para la evaluación de la eficiencia del sistema reformado desde el punto de vista de la elección óptima de Administradora por los partícipes, en dos momentos de la vida del nuevo sistema.

  1. Desarrollo de una Interfaz de Usuario para el Sistema Robótico Multiagente SMART

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    Cecilia García Cena

    2010-10-01

    Full Text Available Resumen: En este artículo se presenta al sistema robótico multi-agente SMART. Este sistema está compuesto por varios tipos de agentes software y/o hardware, por lo que puede clasificárselo como heterogéneo. Además, se presenta el desarrollo de una interfaz de realidad virtual a través de la cual el usuario puede intervenir en el sistema si lo considera necesario, en otro caso el sistema opera autónomamente. Asimismo, esta interfaz implementa un gran número de funcionalidades tendientes a lograr el buen desempeño del sistema, una correcta gestión de los recursos robóticos disponibles en el entorno y provee al usuario información en tiempo real de todo lo que sucede en la realidad. Como consecuencia de la heterogeneidad del sistema, la comunicación entre los diferentes agentes se realiza utilizando diversas tecnologías de comunicación (TCP/IP, WiFi y Bluetooth; por lo tanto, se ha diseñado un protocolo de comunicaciones específico para este sistema. Palabras clave: robots, agentes, inteligencia artificial distribuida, realidad virtual, cooperación

  2. Sistema de salud de Guatemala The health system of Guatemala

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    Víctor Becerril-Montekio

    2011-01-01

    Full Text Available En este trabajo se describen las condiciones de salud y el sistema de salud de Guatemala, incluyendo su estructura y cobertura, sus fuentes de financiamiento, las actividades de rectoría que en él se desarrollan, así como las tareas de generación de información en salud e investigación. También se discuten los esfuerzos por ampliar la cobertura de servicios básicos, sobre todo a las comunidades rurales pobres. Destacan dentro de las innovaciones recientes del sistema guatemalteco de salud el Programa de Extensión de Cobertura de Servicios Básicos y el Programa de Accesibilidad de Medicamentos, así como los acuerdos del Ministerio de Salud con organizaciones de la sociedad civil para prestar servicios básicos en comunidades rurales.This paper describes the health conditions in Guatemala and, in more detail, the characteristics of the Guatemalan health system, including its structure en coverage, its financial sources, the stewardship functions developed by the Ministry of Health, as well as the generation of health information and the development of research activities. It also discusses the recent efforts to extend coverage of essential health services, mostly to poor rural areas.The most recent innovations also discussed in this paper include the Program for the Expansion of Coverage of Essential Services, the Program to Expand Access to Essential Drugs and the agreements between the Ministry of Health and several non-governmental organizations to provide essential services in rural settings.

  3. Sistema de salud de Colombia The health system of Colombia

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    Ramiro Guerrero

    2011-01-01

    Full Text Available En este trabajo se presenta una breve descripción de las condiciones de salud de Colombia y una descripción detallada del sistema colombiano de salud. Esta última incluye una descripción de su estructura y cobertura, sus fuentes de financiamiento, el gasto en salud, los recursos con los que cuenta, quién vigila y evalúa al sector salud y qué herramientas de participación tienen los usuarios. Dentro de las innovaciones más recientes del sistema se incluyen las modificaciones al Plan Obligatorio de Salud y a los montos de la unidad de pago por capitación, la integración vertical entre empresas promotoras de salud y las instituciones prestadoras de servicios, así como el establecimiento de nuevas fuentes de recursos para lograr la universalidad e igualar los planes de beneficios entre los distintos regímenes.This document briefly describes the health conditions of the Colombian population and, in more detail, the characteristics of the Colombian health system. The description of the system includes its structure and coverage; financing sources; expenditure in health; physical material and human resources available; monitoring and evaluation procedures; and mechanisms through which the population participates in the evaluation of the system. Salient among the most recent innovations implemented in the Colombian health system are the modification of the Compulsory Health Plan and the capitation payment unit, the vertical integration of the health promotion enterprises and the institutions in charge of the provision of services and the mobilization of additional resources to meet the objectives of universal coverage and the homologation of health benefits among health regimes.

  4. Sistema de reconocimiento automático del habla

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    Hugo L. Rufiner

    2004-01-01

    Full Text Available Este trabajo es el resultado de un emprendimiento multidisciplinario en el que se utilizaron herramientas de fonética, fisiología del oído y de la percepción auditiva, lingüística, gramática, redes neuronales, modelización, electrónica analógica y digital, computación e inteligencia artificial. Los objetivos principales fueron la investigación y el desarrollo de sistemas de reconocimiento automático del habla en idioma español, capaces de brindar una salida de texto escrito para ser utilizada en un procesador de textos o como entrada de comandos en programas convencionales o interfaces gráficas. Un sistema completo para el reconocimiento automático del habla puede ser dividido en una etapa de procesamiento de la señal, una de clasificación de fonemas y una de modelado del lenguaje. El primer bloque realiza un procesamiento de la señal de habla, acondicionándola y haciendo un análisis espectral-temporal. Luego, la etapa de clasificación procesa la secuencia de espectros para devolver una cadena de símbolos que corresponden a la secuencia de fonemas o dífonos emitidos. El último módulo consiste en un analizador destinado a descifrar las palabras y frases contenidas en las cadenas de símbolos pseudo fonéticos emitidas por el módulo anterior. En este artículo se revisan las diversas líneas investigadas en torno a esos tres módulos básicos.

  5. A novíssima China e o Sistema Internacional

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    Paulo G. Fagundes Visentini

    2011-11-01

    Full Text Available A chegada da China à periferia em desenvolvimento, com uma agenda política e econômica abrangente, inaugura um novo estágio na projeção internacional chinesa e no próprio sistema mundial. Quais são os objetivos dessa Novíssima China em termos de política internacional? Não são poucos os que identificam nas ações chinesas aspirações ambiciosas de dominação mundial, sucedendo os Estados Unidos como liderança do planeta. Em uma manifestação que beira a sinofobia (como outrora o "perigo amarelo", argumentam que seu desenvolvimento almeja concentrar a riqueza mundial em suas mãos, quebrando com a economia das demais nações. Tomando como base as relações estabelecidas com o continente africano, defendemos a hipótese de que Pequim inaugura uma nova etapa na grande política internacional e suplanta a fase em que a Nova China lutava para recuperar sua soberania e desenvolvimento, começando a Novíssima China a transformar o próprio sistema mundial. Para tal, argumentamos que a China busca evitar as hegemonias, tanto a dos Estados Unidos como a dela própria, pois nesse último caso, poderia ter a mesma sorte que a Alemanha nas duas guerras mundiais. Não se trata de uma tarefa fácil, pois a China move-se em meio à fluidez diplomática do período posterior à Guerra Fria e ao envelhecimento do capitalismo contemporâneo em seus centros históricos.

  6. Secuestro de carbono por sistemas agroforestales amazónicos

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    D. Callo Concha

    2002-01-01

    Full Text Available En la amazonía peruana y durante el segundo semestre del año 2000, evaluamos los volúmenes de carbono secuestrado por seis sistemas agroforestales y/o sistemas de uso de la tierra: bosque primario, bosque secundario, café+sombra, silvopastura, pastura y huerto casero (tratamientos, en tres variantes ecológicas (repeticiones: selva alta, media y baja; fragmentando el muestreo en: a. biomasa arbórea, b. biomasa herbácea, c. hojarasca y d. cuatro estratos de suelo. En lo que se refiere a carbono total, el tratamiento parámetro: bosque primario, reteniendo 465.8 tC·ha-1, supera en 58 % a los SAF’s: huerto casero y café+sombra; en 74.3 % al cuarto, SAF silvopastura y 80 % al otro testigo extremo, pastura. Sin embargo, todos estos, más el bosque de regeneración, son estadísticamente iguales e inferiores al bosque primario (P<0.01. Resultó sumidero estable (P<0.01 y considerable, el suelo. Retiene en casi todos los casos -a excepción del bosque primario- más de la mitad del carbono total. Existe una proporcionalidad inversa entre los sumideros arbóreo y edáfico, función de su grado de cobertura arbórea; los aportes de biomasa herbácea y arbustiva (fresca y hojarasca, son pobres, fluctuando entre el 1 y 2 % y los provenientes de árboles muertos, considerables pero oscilantes en los tratamientos bosque primario, bosque secundario y SAF café+sombra. Las diferencias de carbono secuestrado se deben exclusivamente a la proporción de la cobertura otorgada por la biomasa arbórea (P<0.05.

  7. L’adozione del nuovo sistema di riferimento geodetico Italiano

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    Renzo Carlucci

    2012-06-01

    Full Text Available Sulla Gazzetta Ufficiale n. 48 del 27 febbraio 2012 (supplemento ordinario n. 37 è stato pubblicato il DM con cui è stato adottato un nuovo sistema di riferimento denominato ETRF2000 (2008.0. I precedenti sistemi di riferimento, ancora in uso in Italia, sono il Roma40 (più conosciuto come Gauss-Boaga o MonteMario, l'ED50 (European Datum 1950 spesso chiamato semplicemente UTM e l'ETRF89 (WGS84. Molti si chiederanno il perchè e quale sia la motivazione di fondo che ha portato lo Stato italiano ad adottare un quarto sistema di riferimento in meno di 100 anni. Adoption of a new geodetic reference system On  the  Official  Gazette  no.  48  of  27 February  2012 (ordinary  supplement no.  37  was  published    the  Ministerial Decree which was adopted a new geodetic reference   system   called ETRF2000  (2008.0.  The previous  ref-erence systems, still in use in Italy, are the Roma40 (better known as Gauss-Boaga and often called MonteMario, the  ED50  (European  Datum  1950, often called  only  UTM  and  ETRF89 (WGS84. Many will wonder why and what  the  underlying  motivation  that led  the  Italian government  to  take fourth  reference  system  in  less than 100 years.

  8. L’adozione del nuovo sistema di riferimento geodetico Italiano

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    Renzo Carlucci

    2012-06-01

    Full Text Available Sulla Gazzetta Ufficiale n. 48 del 27 febbraio 2012 (supplemento ordinario n. 37 è stato pubblicato il DM con cui è stato adottato un nuovo sistema di riferimento denominato ETRF2000 (2008.0. I precedenti sistemi di riferimento, ancora in uso in Italia, sono il Roma40 (più conosciuto come Gauss-Boaga o MonteMario, l'ED50 (European Datum 1950 spesso chiamato semplicemente UTM e l'ETRF89 (WGS84. Molti si chiederanno il perchè e quale sia la motivazione di fondo che ha portato lo Stato italiano ad adottare un quarto sistema di riferimento in meno di 100 anni.Adoption of a new geodetic reference systemOn  the  Official  Gazette  no.  48  of  27 February  2012 (ordinary  supplement no.  37  was  published    the  Ministerial Decree which was adopted a new geodetic reference   system   called ETRF2000  (2008.0.  The previous  ref-erence systems, still in use in Italy, are the Roma40 (better known as Gauss-Boaga and often called MonteMario, the  ED50  (European  Datum  1950, often called  only  UTM  and  ETRF89 (WGS84. Many will wonder why and what  the  underlying  motivation  that led  the  Italian government  to  take fourth  reference  system  in  less than 100 years.

  9. Sistema de salud de Cuba The health system of Cuba

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    Emma Domínguez-Alonso

    2011-01-01

    Full Text Available En este trabajo se describen las condiciones de salud de Cuba y el sistema cubano de salud, incluyendo su estructura y cobertura, sus fuentes de financiamiento, su gasto en salud, los recursos físicos, materiales y humanos de los que dispone, y las actividades de rectoría e investigación que desarrolla. También se discute la importancia de sus instituciones de investigación y se describe el papel de los usuarios de los servicios en la operación y evaluación del sistema, así como las actividades que en este sentido desarrollan la Federación de Mujeres Cubanas y los Comités de Defensa de la Revolución. La parte final de este trabajo se dedica a discutir las innovaciones más recientes dentro de las que destacan las redes de cardiología, la Misión Milagro y la Batalla de Ideas.This paper describes the health conditions in Cuba and the general characteristics of the Cuban health system, including its structure and coverage, its financial sources, its health expenditure, its physical, material and human resources, and its stewardship functions. It also discusses the increasing importance of its research institutions and the role played by its users in the operation and evaluation of the system. Salient among the social actors involved in the health sector are the Cuban Women Federation and the Committees for the Defense of the Revolution. The paper concludes with the discussion of the most recent innovations implemented in the Cuban health system, including the cardiology networks, the Miracle Mission (Misión Milagro and the Battle of Ideas (Batalla de Ideas.

  10. As Limitações dos Sistemas de Metas

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    Mário Teixeira Reis Neto

    2011-04-01

    Full Text Available Neste ambiente de grandes incertezas em que as organizações estão inseridas, a correta utilização de um sistema de medição pode significar vantagem competitiva e desempenho. Este ensaio procura esclarecer os limites dos sistemas de medição, verificando se a utilização de metas de resultado é suficiente para que uma empresa tenha um desempenho superior. O objetivo é analisar a teoria das metas sob um olhar crítico, observando os limites da utilização de diversos tipos de metas, até mesmo no serviço público. Buscou-se fazer uma análise das pesquisas dos autores que defendem a utilização de metas para se ter desempenho e, a partir das suas premissas, analisar os estudos dos autores que as criticam, mostrando que elas podem causar, dependendo da situação, sérios danos financeiros e à imagem da organização. Após essa análise, verificou-se que as metas possuem um importante papel no aumento do desempenho da organização, todavia, deve-se ter certo cuidado na sua utilização, pois elas podem levar as pessoas a comportamentos não éticos, à inibição da cooperação e do aprendizado organizacional, entre outros.

  11. Sistema de salud de Nicaragua The health system of Nicaragua

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    Jorine Muiser

    2011-01-01

    Full Text Available En este artículo se describen las condiciones de salud de Nicaragua y las características de su sistema de salud, incluyendo su estructura y cobertura, sus fuentes de financiamiento, los recursos físicos, materiales y humanos con los que cuenta, las actividades de rectoría que desarrolla el Ministerio de Salud, los mecanismos de participación ciudadana en la gestión y evaluación de los sistemas de salud, y la satisfacción de los usuarios con los servicios recibidos. También se discuten las innovaciones más recientes, dentro de las que destacan la promulgación de una nueva Ley General de Salud, la descentralización de la regulación de los establecimientos de salud y el diseño de un nuevo modelo de atención a la salud denominado Modelo de Salud Familiar y Comunitario.This paper describes the health conditions in Nicaragua and discusses the characteristics of its national health system including its structure and coverage, its financial sources its physical, material and human resources the stewardship functions developed by the Ministry of Health the participation of citizens in the operation and evaluation of the system and the level of satisfaction of health care users. It also discusses the most recent policy innovations, including the new General Health Law, the decentralization of the regulation of health facilities and the design and implementation of a new health care model known as Family and Community Health Model.

  12. Desarrollo de un sistema experto para el diagnostico de fallas automotrices

    OpenAIRE

    Fuentes Covarrubias, Ricardo; Fuentes Covarrubias, Andrés Gerardo

    2013-01-01

    Se presenta un prototipo experimental de un sistema experto para el diagnóstico de fallas mecánicas en automóviles, a partir de una interacción en tiempo real. Este sistema es una herramienta de diagnóstico para técnicos mecánicos, practicantes y personas con conocimientos básicos de mecánica automotriz y puede también ser empleado como un sistema tutor inteligente para un curso de mecánica automotriz. In this paper we present an experimental prototype of an expert system for the diagnosis...

  13. Sistema de recomendación para un uso inclusivo del lenguaje

    OpenAIRE

    Fuentes Fort, Maria; Padró Cirera, Lluís; Padró Cirera, Montserrat; Turmo Borrás, Jordi; Carrera, Jordi

    2009-01-01

    Sistema que procesa un texto escrito en castellano detectando usos del lenguaje no inclusivos. Para cada sintagma nominal sospechoso el sistema propone una serie de alternativas. El sistema permite también la adquisición automática de ejemplos positivos a partir de documentos que hagan un uso inclusivo del lenguaje. Estos ejemplos seran usados, junto a su contexto, en la presentación de sugerencias. System to detect exclusive language in Spanish documents. For each noun phrase detected as ...

  14. Sistema de recomendación para un uso inclusivo del lenguaje

    OpenAIRE

    Fuentes Fort, Maria; Padró, Lluís; Padró Cirera, Montserrat; Turmo Borras, Jorge; Carrera, Jordi

    2009-01-01

    Sistema que procesa un texto escrito en castellano detectando usos del lenguaje no inclusivos. Para cada sintagma nominal sospechoso el sistema propone una serie de alternativas. El sistema permite también la adquisición automática de ejemplos positivos a partir de documentos que hagan un uso inclusivo del lenguaje. Estos ejemplos serán usados, junto a su contexto, en la presentación de sugerencias. Abstract: System to detect exclusive language in spanish documents. For each ...

  15. Sistemas de cultivo de arroz irrigado e a compactação de um Planossolo

    OpenAIRE

    Pedrotti,Alceu; Pauletto,Eloy Antonio; Gomes,Algenor da Silva; Turatti,Ariovaldo Luis; Crestana,Silvio

    2001-01-01

    Resumo ¾ Em 1993 foi avaliada a compactação de um Planossolo cultivado sob diferentes sistemas de manejo, medindo-se a densidade do solo, por meio do método do anel volumétrico, em três camadas de solo. Os tratamentos consistiram de seis sistemas de manejo, selecionados de um experimento conduzido na Embrapa-Centro de Pesquisa Agropecuária de Clima Temperado, desde 1985. Observaram-se, de maneira geral, valores altos de densidade do solo; na primeira camada de solo (0-10 cm) o sistema que apr...

  16. Balanceo de un sistema de cosecha mecanizado utilizando simulación de eventos discretos.

    OpenAIRE

    Pablo Aracena; Darío Aedo; Ricardo Landeros

    2010-01-01

    Se analizó un sistema de cosecha mecanizado, operando en una faena a tala rasa de pino radiata y conformado por un feller buncher, un skidder con garra, un procesador y un trineumático. Un Modelo de Simulación de Eventos Discretos (MSED) fue desarrollado con el propósito de balancear el sistema. El proceso mecanizado de madera fue el limitante del sistema de acuerdo con los resultados del estudio de tiempos, entonces este proceso fue apoyado agregando a 3 operadores de motosierra para alcanza...

  17. Un modelo de eventos discretos para la simulación de sistemas de tiempo real

    OpenAIRE

    Páez, Francisco E.; Urriza, José M.; Orozco, Javier D.; Buckle, Carlos

    2011-01-01

    En este trabajo se presenta un modelo de eventos discretos para el diseño de simuladores de Sistemas de Tiempo Real. Los simuladores sirven para realizar comprobaciones de algoritmos, modelos, técnicas y para evaluar métricas de rendimiento. El modelo de eventos discretos se ajusta perfectamente a los sistemas dinámicos discretos como lo son los Sistemas de Tiempo Real. El modelo se desarrolla empleando la técnica de grafo de eventos, debido a su sencillez y facilidad de implementación.

  18. Implantación de un sistema de control integral de equipamiento informático

    OpenAIRE

    Valiente Fernández, José Manuel

    2015-01-01

    Cada vez más las empresas, tanto las grandes compañías como las PYMES, se van adaptando a las nuevas tecnologías y requieren una mayor informatización de sus sistemas. Este proceso exige entre otras cosas un control de los sistemas implantados y la securización de la información confidencial de las empresas adecuándose a la legislación vigente. Debido a esto todos los sistemas informáticos requieren de un control, mantenimiento y actualización constante. Estas tareas administrativas de ...

  19. La escuela de sistemas y sus aplicaciones a la administración.

    OpenAIRE

    2013-01-01

    El artículo hace de forma general una caracterización de la Escuela de Sistemas, iniciando con una aproximación conceptual, pasando por una clasificación de los distintos sistemas, sus niveles de desarrollo y presentando una rápida relación entre la teoría de sistemas y la administración, su aplicación, describiendo a manera de ejemplo un modelo referente a una pequeña empresa panificadora.

  20. La escuela de sistemas y sus aplicaciones en la administración

    OpenAIRE

    Carlos Ramírez Cardona

    1989-01-01

    RESUMEN El artículo hace de forma general una caracterización  de la Escuela de Sistemas, iniciando con una aproximación conceptual, pasando por una clasificación de los distintos sistemas, sus niveles de desarrollo y presentando una rápida relación entre la teoría  de sistemas y la administración, su aplicación, describiendo a manera de ejemplo un modelo referente a una pequeña empresa panificadora.