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Sample records for sinh ca tu

  1. Comparison of Plasma Tu-M2-PK and CA19-9 in Pancreatic Cancer

    DEFF Research Database (Denmark)

    Joergensen, Maiken Thyregod; Heegaard, Niels H H; Schaffalitzky de Muckadell, Ove B

    2009-01-01

    alone. The presence of chronic pancreatitis or jaundice causes increased levels of CA19-9 but does not influence Tu-M2-PK. CONCLUSIONS:: Tu-M2-PK was inferior to CA19-9 as marker of PDAC. Tu-M2-PK may have a role in diagnosing PDAC because it is not affected by cholestasis or Lewis phenotype. Neither...

  2. Combined Sinh-Cosh-Gordon equation: Symmetry reductions, exact ...

    African Journals Online (AJOL)

    Combined Sinh-Cosh-Gordon equation: Symmetry reductions, exact solutions and conservation laws. ... In this paper we study the combined sinh-cosh-Gordon equation, which arises in mathematical physics and has a wide range of scientific applications that range from chemical reactions to water surface gravity waves.

  3. Instanton solutions from Abelian sinh-Gordon and Tzitzeica vortices

    Science.gov (United States)

    Contatto, Felipe; Dorigoni, Daniele

    2015-12-01

    We study the Abelian Higgs vortex solutions to the sinh-Gordon equation and the elliptic Tzitzeica equation. Starting from these particular vortices, we construct solutions to the Taubes equation with higher vortex number, on surfaces with conical singularities. We then, analyse more general properties of vortices on such singular surfaces and propose a method to obtain vortices on conifolds from vortices on surfaces of revolution. We apply our method to construct explicit vortex solutions on the Poincaré disk with a conical singularity in the centre, to which we refer as the "hyperbolic cone". We uplift the Abelian sinh-Gordon and Tzitzeica vortex solutions to four dimensions and construct cylindrically symmetric, self-dual Yang-Mills instantons on a non-self-dual (nor anti-self-dual) 4-dimensional Kähler manifold with non-vanishing scalar curvature. The instantons we construct in this way cannot be obtained via a twistorial approach.

  4. Semiclassical versus exact quantization of the Sinh-Gordon model

    Energy Technology Data Exchange (ETDEWEB)

    Grossehelweg, Juliane

    2009-12-15

    In this work we investigate the semiclassics of the Sinh-Gordon model. The Sinh-Gordon model is integrable, its explicit solutions of the classical and the quantum model are well known. This allows for a comprehensive investigation of the semiclassical quantization of the classical model as well as of the semiclassical limit of the exact quantum solution. Semiclassical means in this case that the key objects of quantum theory are constructed as formal power series. A quantity playing an important role in the quantum theory is the Q-function. The purpose of this work is to investigate to what extend the classical integrability of the model admits of a construction of the semiclassical expansion of the Q-function. Therefore we used two conceptual independent approaches. In the one approach we start from the exact nonperturbative solution of the quantum model and calculate the semiclassical limit up to the next to leading order. Thereby we found the spectral curve, as well as the semiclassical expansion of the Q-function and of the eigenvalue of the monodromy matrix. In the other approach we constructed the first two orders of the semiclassical expansion of the Q-function, starting from the classical solution theory. The results of both approaches coincide. (orig.)

  5. Statistical mechanics of double sinh-Gordon kinks

    Science.gov (United States)

    Habib, Salman; Khare, Avinash; Saxena, Avadh

    1998-11-01

    We study the classical thermodynamics of the double sinh-Gordon (DSHG) theory in 1 + 1 dimensions. This model theory has a double well potential V( φ) = ( ζ cosh2 φ- n) 2 when n > ζ, thus allowing for the existence of kinks and antikinks. Though it is non-integrable, the DSHG model is remarkably amenable to analysis. Below we obtain exact single kink and kink lattice solutions as well as the asymptotic kink-antikink interaction. In the continuum limit, finding the classical partition function is equivalent to solving for the ground state of a Schrödinger-like equation obtained via the transfer integral method. For the DSHG model, this equation turns out to be quasi-exactly solvable. We exploit this property to obtain exact energy eigenvalues and wavefunctions for several temperatures both above and below the symmetry breaking transition temperature (provided n = 1, 2, …, 6). The availability of exact results provides an excellent testing ground for large scale Langevin simulations. The probability distribution function (PDF) calculated from Langevin dynamics is found to be in striking agreement with the exact PDF obtained from the ground state wavefunction. This validation points to the utility of a PDF-based computation of thermodynamics utilizing Langevin methods. In addition to the PDF, field-field and field fluctuation correlation functions were computed and also found to be in excellent agreement with the exact results.

  6. Soliton solutions of the generalized sinh-Gordon equation by the ...

    Indian Academy of Sciences (India)

    substituting αm,...,v and the general solutions of eq. (8) into (7) we have more travelling wave solutions of the nonlinear evolution eq. (1). 3. Application to the generalized sinh-Gordon equation. First, consider the following transformation: ξ = λ(x + ct), η = λ (x + a ct) , a = c2,. (9) where λ, c are two parameters to be determined.

  7. Tu voz, tu lugar: proyecto comunicacional

    OpenAIRE

    Machado, Karen Noemí; Mamani, Ileana Vanesa; Montoya, Ana Alejandra

    2015-01-01

    Tu voz, tu lugar” es un proyecto comunicacional realizado en el Centro de Actividades Juveniles (C.A.J) del Colegio Secundario Luis María Preti N°5171. Éste se encuentra vinculado a las cátedras Promoción Cultural Comunitaria y Residencia/Pasantía en el Ámbito de la Promoción Comunitaria de la carrera Tecnicatura en Comunicación Social- UNSa- Sede Tartagal. Este proyecto es el resultado de un diagnostico comunicacional, que se retomó desde un posicionamiento teórico crítico, con el...

  8. Amino acid 489 is encoded by a mutational "hot spot" on the beta 3 integrin chain: the CA/TU human platelet alloantigen system.

    Science.gov (United States)

    Wang, R; McFarland, J G; Kekomaki, R; Newman, P J

    1993-12-01

    A new platelet alloantigen, termed CA, has recently been implicated in a case of neonatal alloimmune thrombocytopenia (NATP) in a Filipino family in Canada. Maternal anti-CA serum reacted with glycoprotein (GP) IIIa and maintained its reactivity after removal of high mannose carbohydrate residues from GPIIIa. The monoclonal antibody (MoAb) AP3 partially blocked binding of anti-CA to GPIIIa, suggesting that the CA polymorphism is proximal to the AP3 epitope. Platelet RNA polymerase chain reaction (PCR) was used to amplify the region of GPIIIa cDNA that encodes this region of the protein. DNA sequence analysis showed a GA nucleotide substitution at base 1564 that results in an arginine (Arg) (CGG)glutamine (Gln) (CAG) polymorphism in amino acid (AA) 489. Further analysis of PCR-amplified genomic DNA from 27 normal individuals showed that AA 489 is encoded by a mutational "hot spot" of the GPIIIa gene, as three different codons for the wild-type Arg489 of GPIIIa were also found. The codon usage for Arg489 was found to be: CGG (63%), CGA (37%), and CGC (Definition of these new molecular variants of the beta 3 integrin chain should prove valuable in the diagnosis of NATP in these two geographically disparate populations, and it may also provide useful genetic markers for examining other pathologic variations of the GPIIb-IIIa complex.

  9. Tu proyecto de vida

    OpenAIRE

    Ramos, Georgina

    2012-01-01

    - Párrafo Inicial - Conociendo a Liliana N. - La intervención de la psicóloga Adriana - Tu proyecto de vida - Referencias - Notas de enseñanza o Resumen o Objetivos o Temas relacionados o Plan de aplicación para el aprendizaje o Análisis o Epílogo

  10. Core TuLiP

    NARCIS (Netherlands)

    Czenko, M.R.; Etalle, Sandro

    2007-01-01

    We propose CoreTuLiP - the core of a trust management language based on Logic Programming. CoreTuLiP is based on a subset of moded logic programming, but enjoys the features of TM languages such as RT; in particular clauses are issued by different authorities and stored in a distributed manner. We

  11. English-Vietnamese Scientific Terminology for High School and Junior College Students. Book 4: Chemistry = Danh Tu Khoa Hoc Anh-Viet Danh Cho Hoc Sinh Trung Hoc Va Sinh Vien Nam Thu Nhat Dai Hoc. Quyen 4: Hoa Hoc.

    Science.gov (United States)

    Khanh-Van, Tran Thi

    Vietnamese students now enrolled in American high schools incur an extremely special need in English. After being taught subject matter disciplines in Vietnamese for many years, and while English is still a foreign language for them, these students are bound to go through two linguistic processes. First, while reading or sitting through lectures…

  12. Asymptotic expansion of a partition function related to the sinh-model

    CERN Document Server

    Borot, Gaëtan; Kozlowski, Karol K

    2016-01-01

    This book elaborates on the asymptotic behaviour, when N is large, of certain N-dimensional integrals which typically occur in random matrices, or in 1+1 dimensional quantum integrable models solvable by the quantum separation of variables. The introduction presents the underpinning motivations for this problem, a historical overview, and a summary of the strategy, which is applicable in greater generality. The core aims at proving an expansion up to o(1) for the logarithm of the partition function of the sinh-model. This is achieved by a combination of potential theory and large deviation theory so as to grasp the leading asymptotics described by an equilibrium measure, the Riemann-Hilbert approach to truncated Wiener-Hopf in order to analyse the equilibrium measure, the Schwinger-Dyson equations and the boostrap method to finally obtain an expansion of correlation functions and the one of the partition function. This book is addressed to researchers working in random matrices, statistical physics or integra...

  13. A new numerical treatment based on Lucas polynomials for 1D and 2D sinh-Gordon equation

    Science.gov (United States)

    Oruç, Ömer

    2018-04-01

    In this paper, a new mixed method based on Lucas and Fibonacci polynomials is developed for numerical solutions of 1D and 2D sinh-Gordon equations. Firstly time variable discretized by central finite difference and then unknown function and its derivatives are expanded to Lucas series. With the help of these series expansion and Fibonacci polynomials, matrices for differentiation are derived. With this approach, finding the solution of sinh-Gordon equation transformed to finding the solution of an algebraic system of equations. Lucas series coefficients are acquired by solving this system of algebraic equations. Then by plugginging these coefficients into Lucas series expansion numerical solutions can be obtained consecutively. The main objective of this paper is to demonstrate that Lucas polynomial based method is convenient for 1D and 2D nonlinear problems. By calculating L2 and L∞ error norms of some 1D and 2D test problems efficiency and performance of the proposed method is monitored. Acquired accurate results confirm the applicability of the method.

  14. OECI TuBaFrost tumor biobanking

    NARCIS (Netherlands)

    Riegman, Peter H. J.; Bosch, Antonio Llombart; Riegman, P. H. J.; Dinjens, W. N. M.; Oomen, M. H. A.; Spatz, A.; Ratcliffe, C.; Knox, K.; Mager, R.; Kerr, D.; Pezzella, F.; van Damme, B.; van de Vijver, M.; van Boven, H.; Morente, M. M.; Alonso, S.; Kerjaschki, D.; Pammer, J.; Lopez-Guerrero, J. A.; Bosch, A. Llombart; Carbone, A.; Gloghini, A.; Teodorovic, I.; Isabelle, M.; Jaminé, D.; Passioukov, A.; Lejeune, S.; Therasse, P.; van Veen, E. B.; Lam, K. H.; Oosterhuis, J. W.

    2008-01-01

    OECI TuBaFrost harbors a complete infrastructure for the exchange of frozen tumor samples between European countries. OECI TuBaFrost consists of: * A code of conduct on how to exchange human residual samples in Europe, * A central database application accessible over the Internet (www.tubafrost.org)

  15. TU electric reactor model verification

    International Nuclear Information System (INIS)

    Willingham, C.E.; Killgore, M.R.

    1989-01-01

    Power reactor benchmark calculations using the code package CASMO-3/SIMULATE-3 have been performed for six cycles of Prairie Island Unit 1. The reload fuel designs for the selected cycles include gadolinia as a burnable absorber, natural uranium axial blankets, and increased water-to-fuel ratio. The calculated results for both low-power physics tests (boron end points, control rod worths, and isothermal temperature coefficients) and full-power operation (power distributions and boron letdown) are compared to measured plant data. These comparisons show that the TU Electric reactor physics models accurately predict important physics parameters for power reactors

  16. One-step purification of E. coli elongation factor Tu

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.; Degn, B

    1993-01-01

    The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one...

  17. Biology=Sinh Vat.

    Science.gov (United States)

    Hung, Nguyen Manh, Ed.

    This volume contains 32 biology self-study learning packets designed primarily for Indochinese students in grades 9 to 12. The materials could be used by "English as a Second Language" teachers who may/may not speak one of the Indochinese languages, or by mainstream teachers who have a number of low-English-proficiency Indochinese students in…

  18. TU Delft Library as a vital link

    NARCIS (Netherlands)

    Van Wezenbeek, W.J.S.M.

    2012-01-01

    The next step is to make the Library Learning Centre a vibrant part of the university campus. A university is by nature a learning and research centre. The university campus of the TU Delft is close to the city of Delft at one side, and Science Port Holland – Technopolis, at the other side. The

  19. Artemisinin and Chinese Medicine as Tu Science.

    Science.gov (United States)

    Fu, Jia-Chen

    2017-09-01

    The story of discovery of artemisinin highlights the diversity of scientific values across time and space. Resituating artemisinin research within a broader temporal framework allows us to understand how Chinese drugs like qinghao came to articulate a space for scientific experimentation and innovation through its embodiment of alternating clusters of meanings associated with tu and yang within scientific discourse. Tu science, which was associated with terms like native, Chinese, local, rustic, mass, and crude, articulated a radical vision of science in the service of socialist revolutionary ideals. Yang science, which signified foreign, Western, elite, and professional, tended to bear the hallmarks of professionalism, transnational networks in education and training, and an emphasis on basic or foundational research. With respect to medical research, the case of artemisinin highlights how the constitution of socialist science as an interplay of tu and yang engendered different scientific values and parameters for scientific endeavor. Modern medical research in Maoist China could harness the productive energies of mass participation to technical expertise in its investigations of Chinese drugs, and under the banner of tu science, it became possible and scientifically legitimate to research Chinese drugs in ways that had previously provoked resistance and controversy. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. 1-concave basis for TU games

    NARCIS (Netherlands)

    Driessen, Theo; Khmelnitskaya, Anna Borisovna; Sales, J.

    2005-01-01

    The first stage of research, twenty years ago, on the subclass of 1-convex TU games dealt with its characterization through some regular core structure. Appealing abstract and practical examples of 1-convex games were missing until now. Both drawbacks are solved. On the one hand, a generating set

  1. Structural studies of EF-Tu complexes

    DEFF Research Database (Denmark)

    Johansen, Jesper Sanderhoff

    2011-01-01

    Protein synthesis is a process vital to all living organisms. Protein synthesis occurs on large ribonucleoprotein complexes called ribosomes. Amino acids (aa) are brought to the ribosome as part of a ternary complex consisting of elongation factor EF-Tu, GTP and aminoacyl-tRNA (aa-tRNA). The GTP...... elongation factor, EF-Ts, catalyse the dissociation of GDP from EF-Tu which in turn allows EF-Tu to bind another GTP molecule. The resulting EF-Tu:GTP complex is then cable of forming a ternary complex with a new aa-tRNA which can then associate with the ribosome. During my time as a Ph. D. student I have...... proteins and 22 tRNAs and 2 rRNAs. The mammalian mitochondria have their own specialised translational system for maintaining the synthesis of the 13 proteins. The mammalian mitochondrial protein synthesis resembles the prokaryotic system more than the cytosolic system from eukaryotes. Some of the t...

  2. English-Vietnamese Scientific Terminology for High School and Junior College Students. Book 3: Geography = Danh Tu Khoa Hoc Anh-Viet Danh Cho Hoc Sinh Trung Hoc Va Sinh Vien Nam Thu Nhat Dai Hoc. Quyen 3: Dia Ly Hoc.

    Science.gov (United States)

    Ngu, Nguyen Khac; Khanh-Van, Tran Thi

    Vietnamese students now enrolled in American high schools incur an extremely special need in English. After being taught subject matter disciplines in Vietnamese for many years, and while English is still a foreign language for them, these students are bound to go through two linguistic processes. First, while reading or sitting through lectures…

  3. English-Vietnamese Scientific Terminology for High School and Junior College Students. Book 5: Natural Science = Danh Tu Khoa Hoc Anh-Viet Danh Cho Hoc Sinh Trung Hoc Va Sinh Vien Nam Thu Nhat Dai Hoc. Quyen 5: Van Vat Hoc.

    Science.gov (United States)

    Tam, Tran Duc; Han, Nguyen Thi

    Vietnamese students now enrolled in American high schools incur an extremely special need in English. After being taught subject matter disciplines in Vietnamese for many years, and while English is still a foreign language for them, these students are bound to go through two linguistic processes. First, while reading or sitting through lectures…

  4. English-Vietnamese Scientific Terminology for High School and Junior College Students. Book 2: Physics = Danh Tu Khoa Hoc Anh-Viet Danh Cho Cac Hoc Sinh Trung Hoc Va Cac Sinh Vien Nam Thu Nhat Dai Hoc. Quyen 2: Vat Ly Hoc.

    Science.gov (United States)

    Duong, Pham Cao

    Vietnamese students now enrolled in American high schools incur an extremely special need in English. After being taught subject matter disciplines in Vietnamese for many years, and while English is still a foreign language for them, these students are bound to go through two linguistic processes. First, while reading or sitting through lectures…

  5. Core TuLiP - Logic Programming for Trust Management

    NARCIS (Netherlands)

    Czenko, M.R.; Etalle, Sandro; Dahl, V.; Niemelä, I.

    2007-01-01

    We propose CoreTuLiP - the core of a trust management language based on Logic Programming. CoreTuLiP is based on a subset of moded logic programming, but enjoys the features of TM languages such as RT; in particular clauses are issued by different authorities and stored in a distributed manner. We

  6. Fish associated with aquatic macrophytes in the Chacororé-Sinhá Mariana Lake system and Mutum River, Pantanal of Mato Grosso, Brazil.

    Science.gov (United States)

    Pacheco, E B; Da-Silva, C J

    2009-02-01

    On the left bank of the Cuibá River, one of the main tributaries of the Pantanal in Mato Grosso, are located the Chacororé and Sinhá Mariana Lakes and the Mutum River, the littoral regions of which are covered with aquatic macrophytes, dominated by the species Eichhornia crassipes and Eichhornia azurea. To examine the ichthyofauna associated with this vegetation, in the year 2003, 3510 fish were collected, distributed among 83 species, and the limnological variables verified near the macrophyte stands where they were sampled. The most abundant and richest order was the Characiformes, followed by the Gymnotiformes and Siluriformes. Analysis of similarity showed a very low, or null, index that the limnological conditions and morphology of the aquatic macrophyte species provide habitat sufficiently homogeneous to give. The limnological conditions and morphology of the aquatic macrophyte species provide a sufficient habitat heterogeneity to give very low, or null, index values. Thus, environmental conservation measures should be more effective in promoting the conservation of the species, given the high levels of biological diversity found in the habitats studied.

  7. Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysis

    DEFF Research Database (Denmark)

    Villa, Elizabeth; Sengupta, Jayati; Trabuco, Leonard G.

    2009-01-01

    of EF-Tu that trigger hydrolysis of GTP, along with key interactions, including those between the sarcin-ricin loop and the P loop of EF-Tu, and between the effector loop of EF-Tu and a conserved region of the 16S rRNA. Our data suggest that GTP hydrolysis on EF-Tu is controlled through a hydrophobic...

  8. Characterization of Elongation Factor Tu of Mycoplasma ovipneumoniae

    Directory of Open Access Journals (Sweden)

    Xuan Zhang, Yue-feng Chu, Ping Zhao, Peng-cheng Gao, Ying He, Nu Wang and Zhong-xin Lu*

    2013-11-01

    Full Text Available Mycoplasma ovipneumoniae is considered as an important pathogen of small ruminants, but its antigenic proteins are not well known so far. In this study, we cloned the EF-Tu gene of M. ovipneumoniae and analyzed the molecular features of the gene and its coding protein for the first time. The gene was then expressed in E.coli and the antigenicity of the coding protein was evaluated as well. The EF-Tu gene of M. ovipneumoniae is 1209 bp in length, encodes 402 amino acids, and shares the highest DNA sequence identity of 87.5% and deduced amino acid sequence identity of 97.8% with those of M. hyopneumoniae, respectively. The recombinant EF-Tu protein can react with the polyclonal antiserum of M. ovipneumoniae and can induce humoral immune responses in mice, which indicated that the EF-Tu may be used as a candidate protein in developing the technologies to control the disease.

  9. Studies of elongation factor Tu in Streptococcus faecium (ATCC 9790)

    Energy Technology Data Exchange (ETDEWEB)

    Bourbeau, P.P.

    1986-01-01

    It has been known for over twenty years that elongation factor Tu (Ef-Tu) is one of the proteins involved in protein synthesis in bacteria. Several years ago, it was proposed that Ef-Tu may, in addition, have other structural functions in bacterial. The author's research has examined the function of Ef-Tu in Streptococcus faecium. Using an antibiotic kirromycin, which specifically inhibits Ef-Tu function, the effects upon a number of cellular parameters were determined. Inhibition of both protein and RNA synthesis was found to be similar to the effect of chloramphenicol. Using the residual division technique for the determination of cell cycle events with both heterogeneous and sucrose gradient fractionated cell populations, a kirromycin sensitive event was detected between 8 min. (Td = 30 min.) and 19 min. (Td = 175 min.) later in the cell cycle than the chloramphenical sensitive event. This suggests that kirromycin is inhibiting a terminal cell cycle event which is in addition to the inhibition of protein synthesis. Purification of Ef-Tu was performed using two different methods: ion exchange and molecular exclusion chromatography; and GDP affinity chromatography. Various schemes were employed to try and obtain optimum cellular fractionation, allowing for both proper separation of ribosomes from the other cellular fractions and retention of enzymatic activity by Ef-Tu as determined by a /sup 3/H-GDP binding assay. Analysis of the cell cycle of S. faecium using the residual division technique was also performed. In addition, certain cell wall antibiotics were used to determine if other cell cycle events could be determined using the residual division technique.

  10. College via iTunes U : TU Delft deelt graag kennis

    NARCIS (Netherlands)

    Suijker, P.

    2010-01-01

    De TU Delft publiceert lezingen, colleges en informatieve films op iTunes U van Apple. De auteur beschrijft waarom de TU Delft gekozen heeft voor iTunes U en op welke manier het project tot stand is gekomen.

  11. TU PATERE LEGEM QUAM IPSE FECISTI

    CERN Multimedia

    Association du personnel

    2010-01-01

    In two judgments of February 2009, the ILO Administrative Tribunal (hereafter ILOAT) upheld CERN’s contract policy. In another case having given rise to a recent judgment (3 February 2010), the same problems, in essence, were raised concerning the illegal injustice which could result from the implementation of the policy in question. This time, the Tribunal decided in favour of our colleague, basing itself on the Latin maxim tu patere legem quam ipse fecisti, according to which any authority is bound by its own rules, as long as such rules have not been amended or abrogated. In fact, the Administration had informed the complainant that his application for a long-term contract would be examined for six slots, when in fact it was examined for only one. This judgment represents a victory for the complainant, for the Staff Association and its perseverance, and also for the principle according to which any organisation must keep its word and act in good faith. It shows that, without reversing its jud...

  12. Trust Management in P2P systems using Standard TuLiP

    NARCIS (Netherlands)

    Czenko, M.R.; Doumen, J.M.; Etalle, Sandro

    2008-01-01

    In this paper we introduce Standard TuLiP - a new logic based Trust Management system. In Standard TuLiP, security decisions are based on security credentials, which can be issued by different entities and stored at different locations. Standard TuLiP directly supports the distributed credential

  13. Trust Management in P2P Systems Using Standard TuLiP

    NARCIS (Netherlands)

    Czenko, M.R.; Doumen, J.M.; Etalle, Sandro

    2008-01-01

    In this paper we introduce Standard TuLiP - a new logic based Trust Management system. In Standard TuLiP, security decisions are based on security credentials, which can be issued by different entities and stored at different locations. Standard TuLiP directly supports the distributed credential

  14. I Passiuna tu Christù - Rito e teatro di una cantica popolare della Grecìa Salentina

    OpenAIRE

    Diana Costa

    2012-01-01

    Abstract – IT Questo articolo si basa sugli esiti di una ricerca di campo condotta nei paesi della Grecìa Salentina,una zona del basso Salento, dove si riscontra una peculiare modalità di esecuzione della cantica popolare grìca conosciuta come I Passiuna tu Christù (La Passione di Cristo). Attorno alla metà degli anni ´90 del ´900 la tradizione della cantica è stata oggetto di un prezioso recupero, fondato sulla memoria e disponibilità di alcuni anziani cantori, che l’ha riportata in vita dop...

  15. Problem-Centered Design and Personal Teaching Style: An Exploratory Study of Youguang Tu's Course on Philosophy of Education

    Science.gov (United States)

    Lei, Hongde

    2016-01-01

    Youguang Tu is a contemporary Chinese philosopher of education. His course on philosophy of education had a significant impact on his students. This exploratory study examines how Tu designed and taught this course. Ultimately, there are two reasons why Tu's course had such a significant influence on his students. The first is that Tu used…

  16. Elongation Factor-Tu (EF-Tu) proteins structural stability and bioinformatics in ancestral gene reconstruction

    Science.gov (United States)

    Dehipawala, Sunil; Nguyen, A.; Tremberger, G.; Cheung, E.; Schneider, P.; Lieberman, D.; Holden, T.; Cheung, T.

    2013-09-01

    A paleo-experimental evolution report on elongation factor EF-Tu structural stability results has provided an opportunity to rewind the tape of life using the ancestral protein sequence reconstruction modeling approach; consistent with the book of life dogma in current biology and being an important component in the astrobiology community. Fractal dimension via the Higuchi fractal method and Shannon entropy of the DNA sequence classification could be used in a diagram that serves as a simple summary. Results from biomedical gene research provide examples on the diagram methodology. Comparisons between biomedical genes such as EEF2 (elongation factor 2 human, mouse, etc), WDR85 in epigenetics, HAR1 in human specificity, DLG1 in cognitive skill, and HLA-C in mosquito bite immunology with EF Tu DNA sequences have accounted for the reported circular dichroism thermo-stability data systematically; the results also infer a relatively less volatility geologic time period from 2 to 3 Gyr from adaptation viewpoint. Comparison to Thermotoga maritima MSB8 and Psychrobacter shows that Thermus thermophilus HB8 EF-Tu calibration sequence could be an outlier, consistent with free energy calculation by NUPACK. Diagram methodology allows computer simulation studies and HAR1 shows about 0.5% probability from chimp to human in terms of diagram location, and SNP simulation results such as amoebic meningoencephalitis NAF1 suggest correlation. Extensions to the studies of the translation and transcription elongation factor sequences in Megavirus Chiliensis, Megavirus Lba and Pandoravirus show that the studied Pandoravirus sequence could be an outlier with the highest fractal dimension and lowest entropy, as compared to chicken as a deviant in the DNMT3A DNA methylation gene sequences from zebrafish to human and to the less than one percent probability in computer simulation using the HAR1 0.5% probability as reference. The diagram methodology would be useful in ancestral gene

  17. Purification, characterization, and sequencing of novel antimicrobial peptides, Tu-AMP 1 and Tu-AMP 2, from bulbs of tulip (Tulipa gesneriana L.).

    Science.gov (United States)

    Fujimura, Masatoshi; Ideguchi, Mineo; Minami, Yuji; Watanabe, Keiichi; Tadera, Kenjiro

    2004-03-01

    Novel antimicrobial peptides (AMP), designated Tu-AMP 1 and Tu-AMP 2, were purified from the bulbs of tulip (Tulipa gesneriana L.) by chitin affinity chromatography and reverse-phase high-performance liquid chromatography (HPLC). They bind to chitin in a reversible way. They were basic peptides having isoelectric points of over 12. Tu-AMP 1 and Tu-AMP 2 had molecular masses of 4,988 Da and 5,006 Da on MALDI-TOF MS analysis, and their extinction coefficients of 1% aqueous solutions at 280 nm were 3.3 and 3.4, respectively. Half of all amino acid residues of Tu-AMP 1 and Tu-AMP 2 were occupied by cysteine, arginine, lysine, and proline. The concentrations of peptides required for 50% inhibition (IC(50)) of the growth of plant pathogenic bacteria and fungi were 2 to 20 microg/ml. The structural characteristics of Tu-AMP 1 and Tu-AMP 2 indicated that they were novel thionin-like antimicrobial peptides, though Tu-AMP 2 was a heterodimer composes of two short peptides joined with disulfide bonds.

  18. Rule based design workshop at the TU Berlin

    DEFF Research Database (Denmark)

    Tamke, Martin; Palz, Norbert; Josefsson, Kristoffer

    A new cluster of a possible series of digital design & digital fabrication workshops held at the Berlin University of Technology (TU Berlin), Department of Building Design, School of Architecture, explores teaching and learning Rule Based Design. The first workshop introduced computational logical...

  19. Defining a Pedagogical Model: The TU Delft Online Learning Experience

    NARCIS (Netherlands)

    Ribeiro Jorge, N.; Dopper, S.M.; van Valkenburg, W.F.

    2015-01-01

    In early 2014, the Delft University of Technology (TU Delft) started an innovation program with the aim to respond even more effectively to recent developments in open and online education. Drawing on the fields of Distance Education research and the university’s vision of the “engineer of the

  20. Roles of Protein Synthesis Elongation Factor EF-Tu in Heat Tolerance in Plants

    Directory of Open Access Journals (Sweden)

    Jianming Fu

    2012-01-01

    Full Text Available EF-Tu proteins of plastids, mitochondria, and the cytosolic counterpart EF-1α in plants, as well as EF-Tu proteins of bacteria, are highly conserved and multifunctional. The functions of EF-Tu include transporting the aminoacyl-tRNA complex to the A site of the ribosome during protein biosynthesis; chaperone activity in protecting other proteins from aggregation caused by environmental stresses, facilitating renaturation of proteins when conditions return to normal; displaying a protein disulfide isomerase activity; participating in the degradation of N-terminally blocked proteins by the proteasome; eliciting innate immunity and triggering resistance to pathogenic bacteria in plants; participating in transcription when an E. coli host is infected with phages. EF-Tu genes are upregulated by abiotic stresses in plants, and EF-Tu plays important role in stress responses. Expression of a plant EF-Tu gene confers heat tolerance in E. coli, maize knock-out EF-Tu null mutants are heat susceptible, and over-expression of an EF-Tu gene improves heat tolerance in crop plants. This review paper summarizes the current knowledge of EF-Tu proteins in stress responses in plants and progress on application of EF-Tu for developing crop varieties tolerant to abiotic stresses, such as high temperatures.

  1. Preparation of Immobilized Recombinant Tubulin Beta(TuBb on Chitosan Nanoparticles by Covalent Binding Method

    Directory of Open Access Journals (Sweden)

    Yan Qingfang

    2016-01-01

    Full Text Available Objective In order to screen TuBb inhibitors, this paper describes the preparation of immobilized TuBb on chitosan nanoparticles. Methods TuBb was immobilized onto chitosan nanoparticles by covalent binding method. Results The results of the univariate test indicated that the highest immobilized yield can be obtained when the optimal immobilization condition was 1 mg of TuBb, 0.5 mol/L of buffer solution with pH 6.5, immobilization 30 min and immobilization at 0-4 °C. Conclusions The authors conclude that the immobilized TuBb maintain the catalysis properties and can be used as the screening of TuBb inhibitors.

  2. Trust Management in P2P systems using Standard TuLiP

    OpenAIRE

    Czenko, M.R.; Doumen, J.M.; Etalle, Sandro

    2008-01-01

    In this paper we introduce Standard TuLiP - a new logic based Trust Management system. In Standard TuLiP, security decisions are based on security credentials, which can be issued by different entities and stored at different locations. Standard TuLiP directly supports the distributed credential storage by providing a sound and complete Lookup and Inference AlgoRithm (LIAR). In this paper we focus on (a) the language of Standard TuLiP and (b) on the practical considerations which arise when d...

  3. Production of resonances in the T+U region

    CERN Document Server

    Duboc, J

    1974-01-01

    Preliminary results about K pi pi and KK pi production of resonances in pp annihilations are presented. The p momentum covers the range 1.28-2.04 GeV/c and spans the T+U region. The sample of about 26000 events at present on DST corresponds roughly to 90% of the total statistics and has been limited to events with at least one observed V /sup 0/. (2 refs).

  4. Co-ordination of Mobile Information Agents in TuCSoN.

    Science.gov (United States)

    Omicini, Andrea; Zambonelli, Franco

    1998-01-01

    Examines mobile agent coordination and presents TuCSoN, a coordination model for Internet applications based on mobile information agents that uses a tuple centre, a tuple space enhanced with the capability of programming its behavior in response to communication events. Discusses the effectiveness of the TuCSoN model in the contexts of Internet…

  5. Contribution of Arg288 of Escherichia coli elongation factor Tu to translational functionality

    DEFF Research Database (Denmark)

    Rattenborg, Thomas; Nautrup-Pedersen, Gitte; Clark, Brian F. C.

    1997-01-01

    The recently solved structure of the ternary complex formed between GTP-bound elongation factor Tu and aminoacylated tRNA reveals that the elements of aminoacyl-tRNA that interact with elongation factor Tu can be divided into three groups: the T stem; the 3'-end CCA-Phe; and the 5' end. The conse...

  6. The Danish National Travel Survey - declaration of variables : TU 2006-13, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    This record documents the reported data in the data set TU 2006-13 version 2 covering data from the period May 2006 until 30. April 2014. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  7. The Danish National Travel Survey - declaration of variables TU 2006-11, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar

    This record documents the reported data in the data set TU 2006-11 version 2 covering data from the period May 2006 until 30. April 2012. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  8. The Danish National Travel Survey - declaration of variables : TU 2006-12, version 1

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    This record documents the reported data in the data set TU 2006-12 version 1 covering data from the period May 2006 until 31. December 2012. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  9. The Danish National Travel Survey - declaration of variables : TU 2006-10, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Haunstrup, Brian

    This record documents the reported data in the data set TU 2006-10 version 1 covering data from the period May 2006 until 30 April 2011. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  10. The Danish National Travel Survey - declaration of variables TU 2006-11, version 1

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar

    This record documents the reported data in the data set TU 2006-11 version 1 covering data from the period May 2006 until 31 December 2011. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  11. The Danish National Travel Survey - declaration of variables TU 2006-12, version 2 : Documentation note

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    This record documents the reported data in the data set TU 2006-12 version 1 covering data from the period May 2006 until 3o April 2013. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  12. Monolītu materiāli hromatogrāfijai

    OpenAIRE

    Picka, Edžus

    2010-01-01

    Aplūkotas literatūrā aprakstītās monolītu īpašības, izgatavošanas metodes un to apstākļi, kā arī monolītu izmantošanas iespējas hromatogrāfijā. Darbā veikta metakrilātu monomēru kapilāro monolītu kolonnu izgatavošana ar termisko polimerizāciju un fotopolimerizāciju, kā arī veikta hromatogrāfiskā analīze, izmantojot fotopolimerizācijas ceļā iegūto monolītu kolonnu.

  13. The gammaTuRC Nanomachine Mechanism and Future Applications

    Science.gov (United States)

    Riehlman, Timothy D.

    The complexity and precision of the eukaryotic cell's cytoskeletal network is unrivaled by any man-made systems, perfected by billions of years of evolution, mastering elegant processes of self-assembly, error correction, and self-repair. Understanding the capabilities of these networks will have important and far reaching applications in human medicine by aiding our understanding of developmental processes, cellular division, and disease mechanisms, and through biomimicry will provide insights for biosynthetic manufacturing at the nanoscale and across scales. My research utilizes cross species techniques from Human to the model organism of Fission Yeast to investigate the structure and mechanisms of the g-tubulin ring complex (gTuRC). The gTuRC is a highly conserved eukaryotic multiprotein complex serving as a microtubule organizing center (MTOC) responsible for microtubule nucleation through templating, regulation of dynamics, and establishment of microtubule polarity. Microtubules are 25 nm diameter dynamic flexible polymers of a/b-tubulin heterodimers that function as scaffolds, force generators, distributors, and intracellular highways. The microtubule cytoskeleton is essential for numerous fundamental cellular processes such as mitotic division of chromosomes and cell division, organelle distribution within the cell, cell signaling, and cell shape. This incredible diversity in functions is made possible in part due to molecular motor Kinesin-like proteins (Klps), which allow expansion into more specialized neural, immune, and ciliated cell functions. Combined, the MTOC, microtubules, and Klps represent ideal microtubule cytoskeleton protein (MCP) modular components for in vitro biomimicry towards generation of adaptable patterned networks for human designed applications. My research investigates the hypothesis that a mechanistic understanding of conserved MTOC gTuRC mechanisms will help us understand dynamic cellular nanomachines and their ability to self

  14. Alteration of nucleoside diphosphate binding specificity of E. coli elongation factor Tu (EF-Tu) by single amino acid substitution at position 138

    International Nuclear Information System (INIS)

    Hwang, Y.; Miller, D.L.

    1987-01-01

    A single amino acid substitution (Asp → Asn) at position 138 of E. coli EF-Tu was induced in the tufA gene by an M13 phage oligonucleotide site-directed mutagenesis protocol. The mutated tufA gene was then subcloned in a plasmid vector and expressed in maxicells. The properties of [ 35 S]methionine labelled mutant and wild type EF-Tu's were compared by in vitro assays. Mutant and wild-type EF-Tu's bound EF-Ts with approximately equal affinities. The 138-Asn mutation greatly reduced the protein's affinity for GDP; however, this mutation dramatically increased the proteins affinity for XDP. The mutant protein forms a stable complex with phe-tRNA and XTP, which binds to ribosomes; whereas, it does not form a complex with phe-tRNA and GTP. These results suggest that in EF-Tu x NDP complexes amino acid residue 138 must interact with the substituent on C-2 of the purine ring. Thus in wild-type EF-Tu Asp-138 would H-bond to 2-NH 2 of GDP, and in the mutant EF-Tu ASN-138 would form an equivalent H-bond with 2-0 of XDP. This explains why Asp-138 is conserved in the guanosine binding domains of nearly all GDP regulatory proteins

  15. The Measure your World / Mide tu Mundo Project

    Science.gov (United States)

    Hojman, S.; Johnson, R. M.; Meymaris, K. K.; Ward, D. L.; Russell, R.; Genyuk, J.; Lagrave, M.; Henderson, S.; Ostrosky, J.; Martinez, M.

    2007-12-01

    Over 22 centuries ago Erathostenes devised a method to determine the Earth radius. Measure your World / Mide tu Mundo is a joint venture lead by Windows to the Universe/Ventanas al Universo in the United States of America, EducaRed in Chile and RedEscolar in Mexico seeking to partner teams of students, teachers and parents from the three countries in a collaborative effort to determine the Earth circumference by sharing the results of their measurements, and exchanging cultural information. Data are collected from September 29 through October 7, 2007. A report of the whole experience will be presented.

  16. TU Electric reactor physics model verification: Power reactor benchmark

    International Nuclear Information System (INIS)

    Willingham, C.E.; Killgore, M.R.

    1988-01-01

    Power reactor benchmark calculations using the advanced code package CASMO-3/SIMULATE-3 have been performed for six cycles of Prairie Island Unit 1. The reload fuel designs for the selected cycles included gadolinia as a burnable absorber, natural uranium axial blankets and increased water-to-fuel ratio. The calculated results for both startup reactor physics tests (boron endpoints, control rod worths, and isothermal temperature coefficients) and full power depletion results were compared to measured plant data. These comparisons show that the TU Electric reactor physics models accurately predict important measured parameters for power reactors

  17. Doc toxin is a kinase that inactivates elongation factor Tu.

    Science.gov (United States)

    Cruz, Jonathan W; Rothenbacher, Francesca P; Maehigashi, Tatsuya; Lane, William S; Dunham, Christine M; Woychik, Nancy A

    2014-03-14

    The Doc toxin from bacteriophage P1 (of the phd-doc toxin-antitoxin system) has served as a model for the family of Doc toxins, many of which are harbored in the genomes of pathogens. We have shown previously that the mode of action of this toxin is distinct from the majority derived from toxin-antitoxin systems: it does not cleave RNA; in fact P1 Doc expression leads to mRNA stabilization. However, the molecular triggers that lead to translation arrest are not understood. The presence of a Fic domain, albeit slightly altered in length and at the catalytic site, provided a clue to the mechanism of P1 Doc action, as most proteins with this conserved domain inactivate GTPases through addition of an adenylyl group (also referred to as AMPylation). We demonstrated that P1 Doc added a single phosphate group to the essential translation elongation factor and GTPase, elongation factor (EF)-Tu. The phosphorylation site was at a highly conserved threonine, Thr-382, which was blocked when EF-Tu was treated with the antibiotic kirromycin. Therefore, we have established that Fic domain proteins can function as kinases. This distinct enzymatic activity exhibited by P1 Doc also solves the mystery of the degenerate Fic motif unique to the Doc family of toxins. Moreover, we have established that all characterized Fic domain proteins, even those that phosphorylate, target pivotal GTPases for inactivation through a post-translational modification at a single functionally critical acceptor site.

  18. Doc Toxin Is a Kinase That Inactivates Elongation Factor Tu*

    Science.gov (United States)

    Cruz, Jonathan W.; Rothenbacher, Francesca P.; Maehigashi, Tatsuya; Lane, William S.; Dunham, Christine M.; Woychik, Nancy A.

    2014-01-01

    The Doc toxin from bacteriophage P1 (of the phd-doc toxin-antitoxin system) has served as a model for the family of Doc toxins, many of which are harbored in the genomes of pathogens. We have shown previously that the mode of action of this toxin is distinct from the majority derived from toxin-antitoxin systems: it does not cleave RNA; in fact P1 Doc expression leads to mRNA stabilization. However, the molecular triggers that lead to translation arrest are not understood. The presence of a Fic domain, albeit slightly altered in length and at the catalytic site, provided a clue to the mechanism of P1 Doc action, as most proteins with this conserved domain inactivate GTPases through addition of an adenylyl group (also referred to as AMPylation). We demonstrated that P1 Doc added a single phosphate group to the essential translation elongation factor and GTPase, elongation factor (EF)-Tu. The phosphorylation site was at a highly conserved threonine, Thr-382, which was blocked when EF-Tu was treated with the antibiotic kirromycin. Therefore, we have established that Fic domain proteins can function as kinases. This distinct enzymatic activity exhibited by P1 Doc also solves the mystery of the degenerate Fic motif unique to the Doc family of toxins. Moreover, we have established that all characterized Fic domain proteins, even those that phosphorylate, target pivotal GTPases for inactivation through a post-translational modification at a single functionally critical acceptor site. PMID:24448800

  19. 40Ar/39Ar Geochronology of Supergene K-bearing Sulfate Minerals: Cenozoic Continental Weathering, Landscape Evolution and Paleoclimates in the Tu-Ha Basin, Northwestern China

    Science.gov (United States)

    Yang, J.; Zheng, D.; Chen, W.; Hough, B.; Qiu, H.; Wang, W.; Wu, Y.; Yang, L.

    2017-12-01

    40Ar/39Ar incremental-heating analyses of supergene jarosite and yavapaiite from three weathering profiles at the Hongshan, Liuhuangshan, and Caihuagou deposits in the Tu-Ha Basin, China, were carried out to provide numerical constraints on the timing and duration of weathering and derive insights into local paleoclimatic and landscape evolution. Well-constrained plateau ages and best-fitting inverse isochrons have been obtained for 11 samples, yield 40Ar/39Ar ages ranging from 33.3±0.5 Ma to 3.3±0.4 Ma (1σ). Our 40Ar/39Ar ages, combined with the published ages, indicate that the high elevations sites hosting the most complete and complex weathering profiles present. The presence of ancient weathering ages in current outcrop in the Tu-Ha Basin suggests that denudation was not homogeneous, and the landscape evolution follow a scarp retreat model. These geochronological results suggest that a protracted history of weathering and supergene enrichment and, by inference, arid-semiarid climate (with at least a moderate amount of precipitation (>10 cm/y)) favorable to intense chemical weathering emerged at 33.3 Ma, 27.7-23.3 Ma, and 16.4-14.7 Ma, and prevailed from 11-7.8 Ma. Then, a progressive change from arid-semiarid towards hyperarid conditions and predominantly hyperarid conditions may have persisted since at least ca. 3.3 Ma. The climatic implications inferred from the weathering geochronology are in agreement with the chemical parameters and isotopic compositions of the Cenozoic sedimentary sequence from the Lianmuqin section in the Tu-Ha Basin, attesting to the reliability of weathering geochronology by the 40Ar/39Ar method as an indicator of paleoclimate in arid areas. Our results suggest that the retreat of the Paratethys Sea, which would have reduced eastward water vapor transport by the westerlies to the Tu-Ha Basin, led to its aridification in the Oligocene and that increased rain shadow effects, resulting from uplift of the Tibetan Plateau and Tian Shan

  20. Genetic control of immunity to Turnip mosaic virus (TuMV) pathotype 1 in Brassica rapa (Chinese cabbage).

    Science.gov (United States)

    Lydiate, Derek J; Pilcher, Rachel L Rusholme; Higgins, Erin E; Walsh, John A

    2014-08-01

    Turnip mosaic virus (TuMV) is the major virus infecting crops of the genus Brassica worldwide. A dominant resistance gene, TuRB01b, that confers immunity to the virus isolate UK 1 (a representative pathotype 1 isolate of TuMV) on Brassica rapa was identified in the Chinese cabbage cultivar Tropical Delight. The TuRB01b locus was mapped to a 2.9-cM interval on B. rapa chromosome 6 (A6) that was flanked by RFLP markers pN101e1 and pW137e1. This mapping used a first backcross (B(1)) population segregating for the resistance gene at TuRB01b and sets of RFLP markers employed in previous mapping experiments in Brassica. Virus-plant interaction phenotypes were assayed in inbred progeny derived from B(1) individuals to allow different virus isolates to be tested. Comparative mapping confirmed that A6 of B. rapa was equivalent to chromosome 6 of Brassica napus (A6) and that the map position of TuRB01b in B. rapa could be identical to that of TuRB01 in B. napus. Detailed evaluation of plant-virus interactions showed that TuRB01 and TuRB01b had indistinguishable specificities to a range of TuMV isolates. The possibility that TuRB01 and TuRB01b represent similar or identical alleles at the same A genome resistance locus suggests that B. napus acquired TuRB01 from the B. rapa gene pool.

  1. Projekt Öko-Audit an der TU Dresden - erste Ergebnisse

    OpenAIRE

    Fröhlich, Jürgen; Günther, Edeltraud; Jetschny, Wolfgang; Klauke, Ines; Winkler, Ute

    2001-01-01

    Zwischenbericht zum Projekt “Multiplikatorwirkung und Implementierung des Öko-Audits an Hochschuleinrichtungen nach EMAS II am Beispiel der TU Dresden“, gefördert durch die Deutsche Bundesstiftung Umwelt

  2. Tunable Light-Guide Image Processing Snapshot Spectrometer (TuLIPSS) for Earth and Moon Observations

    Science.gov (United States)

    Tkaczyk, T. S.; Alexander, D.; Luvall, J. C.; Wang, Y.; Dwight, J. G.; Pawlowsk, M. E.; Howell, B.; Tatum, P. F.; Stoian, R.-I.; Cheng, S.; Daou, A.

    2018-02-01

    A tunable light-guide image processing snapshot spectrometer (TuLIPSS) for Earth science research and observation is being developed through a NASA instrument incubator project with Rice University and Marshall Space Flight Center.

  3. The intensive DT neutron generator of TU Dresden

    Science.gov (United States)

    Klix, Axel; DÖring, Toralf; Domula, Alexander; Zuber, Kai

    2018-01-01

    TU Dresden operates an accelerator-based intensive DT neutron generator. Experimental activities comprise investigation into material activation and decay, neutron and photon transport in matter and R&D work on radiation detectors for harsh environments. The intense DT neutron generator is capable to produce a maximum of 1012 n/s. The neutron source is a solid-type water-cooled tritium target based on a titanium matrix on a copper carrier. The neutron yield at a typical deuteron beam current of 1 mA is of the order of 1011 n/s in 4Π. A pneumatic sample transport system is available for short-time irradiations and connected to wo high-purity germanium detector spectrometers for the measurement of induced activities. The overall design of the experimental hall with the neutron generator allows a flexible setup of experiments including the possibility of investigating larger structures and cooled samples or samples at high temperatures.

  4. Cloning and Characterization of EF-Tu and EF-Ts from Pseudomonas aeruginosa

    Directory of Open Access Journals (Sweden)

    Stephanie O. Palmer

    2013-01-01

    Full Text Available We have cloned genes encoding elongation factors EF-Tu and EF-Ts from Pseudomonas aeruginosa and expressed and purified the proteins to greater than 95% homogeneity. Sequence analysis indicated that P. aeruginosa EF-Tu and EF-Ts are 84% and 55% identical to E. coli counterparts, respectively. P. aeruginosa EF-Tu was active when assayed in GDP exchange assays. Kinetic parameters for the interaction of EF-Tu with GDP in the absence of EF-Ts were observed to be = 33 μM, = 0.003 s−1, and the specificity constant was  s−1 μM−1. In the presence of EF-Ts, these values were shifted to = 2 μM, = 0.005 s−1, and the specificity constant was  s−1 μM−1. The equilibrium dissociation constants governing the binding of EF-Tu to GDP ( were 30–75 nM and to GTP ( were 125–200 nM. EF-Ts stimulated the exchange of GDP by EF-Tu 10-fold. P. aeruginosa EF-Tu was active in forming a ternary complex with GTP and aminoacylated tRNA and was functional in poly(U-dependent binding of Phe-tRNAPhe at the A-site of P. aeruginosa ribosomes. P. aeruginosa EF-Tu was active in poly(U-programmed polyphenylalanine protein synthesis system composed of all P. aeruginosa components.

  5. Function and structure in phage Qbeta RNA replicase. Association of EF-Tu-Ts with the other enzyme subunits

    DEFF Research Database (Denmark)

    Blumenthal, T; Young, R A; Brown, S

    1976-01-01

    of EF-Tu-Ts from the other enzyme subunits: whereas free EF-Tu-Ts binds GDP independently of salt concentration, this binding by Qbeta replicase is sensitive to high ionic strength and remains so in the presence of Qbeta RNA. Furthermore, RNA does not allow the release of EF-Ts from EF-Tu by GTP......Qbeta replicase is a complex of four nonidentical subunits readily dissociable into two subcomplexes: 30 S ribosomal protein S1 and the phage-coded polypeptide (Subunits I + II) and protein synthesis elongation factors EF-Tu and EF-Ts (Subunits III + IV). The affinity of the two subcomplexes...

  6. A kirromycin-resistant EF-Tu species reverses streptomycin dependence of Escherichia coli strains mutated in ribosomal protein S12.

    Science.gov (United States)

    Zuurmond, A M; Zeef, L A; Kraal, B

    1998-12-01

    Streptomycin dependence can be caused by mutations in ribosomal protein S12. Mutations suppressing such streptomycin dependence have been found in ribosomal proteins S4 and S5, and in 16S rRNA. Here a new suppressor mutation localized in elongation factor Tu (EF-Tu) is described, consistent with recent models of ribosome-EF-Tu-tRNA interaction at the decoding centre. The EF-Tu mutation was obtained by genetic selection for streptomycin independence; it was identified as Ala375 --> Thr, previously described as EF-TuA(R) and known to confer a kirromycin-resistant, error-prone phenotype. Also, other streptomycin-dependent (SmD) S12 mutations could be complemented by this mutation. The streptomycin-independent (Sm1) strain grows more slowly than the wild-type (wt), suggesting that not all the defects of the S12 mutation can be complemented by EF-Tu[A375T]. Moreover, this strain is more susceptible than wt to reduction in the cellular EF-Tu concentration, and disruption of tufB led to considerable growth-rate impairment. Expression of EF-Tu from tufB, not only of wt EF-Tu and EF-Tu[A375T] but, remarkably, also of EF-Tu[G222D], known as EF-TuB0 and defective in protein synthesis, equally contributed to cell growth. In vitro analysis revealed a decreased translational activity of wt EF-Tu with SmD ribosomes as compared to EF-Tu[A375T], while EF-Tu[G222D] showed no activity at all, just as with wt ribosomes. Possible mechanisms are discussed for the improved growth rate observed in such Sm1 strains when they include wt EF-Tu or EF-Tu[G222D].

  7. Independent safety analysis - why us? - A perspective from TU electric

    International Nuclear Information System (INIS)

    Rice, B.L.; Boatwright, W.J.; De Vore, C.V.; Lo, S.S.; Maier, S.M.

    1988-01-01

    The development of a plant transient analysis capability at TU Electric was initiated in 1982 for Comanche Peak operations support. This type of support was available from the NSSS vendor, but the timeliness and cost of vendor responses to requests for services become a concern. RETRAN-02 MOD002 was acquired at that time for developing a best estimate model of Comanche Peak Unit 1. The activities of the Transient Analysis Section have evolved to a point where operations support is no longer the primary activity. RETRAN is also being used for the development of reload licensing methods and the resolution of licensing concerns raised internally or by the Nuclear Regulatory Commission (NRC). Future uses of RETRAN include simulator certification, as required by Regulatory Guide 1.149, and Justifications for Continued Operation for the plants. Benefits similar to those experienced through the RETRAN activities are expected in the following areas currently being developed: core thermal-hydraulics, fuel performance, LOCA, and systems analysis (PRA and reliability analysis). The major benefit expected from these activities is the development of a thorough understanding of plant operations and the in-house ability to handle operations and licensing questions, concerns and problems in a timely and cost effective manner

  8. The intensive DT neutron generator of TU Dresden

    Directory of Open Access Journals (Sweden)

    Klix Axel

    2018-01-01

    Full Text Available TU Dresden operates an accelerator-based intensive DT neutron generator. Experimental activities comprise investigation into material activation and decay, neutron and photon transport in matter and R&D work on radiation detectors for harsh environments. The intense DT neutron generator is capable to produce a maximum of 1012 n/s. The neutron source is a solid-type water-cooled tritium target based on a titanium matrix on a copper carrier. The neutron yield at a typical deuteron beam current of 1 mA is of the order of 1011 n/s in 4Π. A pneumatic sample transport system is available for short-time irradiations and connected to wo high-purity germanium detector spectrometers for the measurement of induced activities. The overall design of the experimental hall with the neutron generator allows a flexible setup of experiments including the possibility of investigating larger structures and cooled samples or samples at high temperatures.

  9. Preclinical trials in autosomal dominant AD: implementation of the DIAN-TU trial.

    Science.gov (United States)

    Mills, S M; Mallmann, J; Santacruz, A M; Fuqua, A; Carril, M; Aisen, P S; Althage, M C; Belyew, S; Benzinger, T L; Brooks, W S; Buckles, V D; Cairns, N J; Clifford, D; Danek, A; Fagan, A M; Farlow, M; Fox, N; Ghetti, B; Goate, A M; Heinrichs, D; Hornbeck, R; Jack, C; Jucker, M; Klunk, W E; Marcus, D S; Martins, R N; Masters, C M; Mayeux, R; McDade, E; Morris, J C; Oliver, A; Ringman, J M; Rossor, M N; Salloway, S; Schofield, P R; Snider, J; Snyder, P; Sperling, R A; Stewart, C; Thomas, R G; Xiong, C; Bateman, R J

    2013-10-01

    The Dominantly Inherited Alzheimer's Network Trials Unit (DIAN-TU) was formed to direct the design and management of interventional therapeutic trials of international DIAN and autosomal dominant Alzheimer's disease (ADAD) participants. The goal of the DIAN-TU is to implement safe trials that have the highest likelihood of success while advancing scientific understanding of these diseases and clinical effects of proposed therapies. The DIAN-TU has launched a trial design that leverages the existing infrastructure of the ongoing DIAN observational study, takes advantage of a variety of drug targets, incorporates the latest results of biomarker and cognitive data collected during the observational study, and implements biomarkers measuring Alzheimer's disease (AD) biological processes to improve the efficiency of trial design. The DIAN-TU trial design is unique due to the sophisticated design of multiple drugs, multiple pharmaceutical partners, academics servings as sponsor, geographic distribution of a rare population and intensive safety and biomarker assessments. The implementation of the operational aspects such as home health research delivery, safety magnetic resonance imagings (MRIs) at remote locations, monitoring clinical and cognitive measures, and regulatory management involving multiple pharmaceutical sponsors of the complex DIAN-TU trial are described. Published by Elsevier Masson SAS.

  10. The YvfTU Two-component System is involved in plcR expression in Bacillus cereus

    Directory of Open Access Journals (Sweden)

    Nguyen-the Christophe

    2008-10-01

    Full Text Available Abstract Background Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative role of YvfTU in the expression of the PlcR regulon was therefore investigated. Results Expression of the plcR gene was monitored using a transcriptional fusion with a lacZ reporter gene in a yvfTU mutant and in its B. cereus ATCC 14579 parental strain. Two hours after the onset of the stationary phase, a stage at which the PlcR regulon is highly expressed, the plcR expression in the yvfTU mutant was only 50% of that of its parental strain. In addition to the reduced plcR expression in the yvfTU mutant, a few members of the PlcR regulon showed a differential expression, as revealed by transcriptomic and proteomic analyses. The virulence of the yvfTU mutant in a Galleria mellonella insect model was slightly lower than that of the parental strain. Conclusion The YvfTU two-component system is not required for the expression of most of the virulence factors belonging to the PlcR regulon. However, YvfTU is involved in expression of plcR, a major regulator of virulence in B. cereus.

  11. Mutation of the conserved Gly83 and Gly94 in Escherichia coli elongation factor Tu. Indication of structural pivots

    DEFF Research Database (Denmark)

    Kjaersgård, I V; Knudsen, Charlotte Rohde; Wiborg, O

    1995-01-01

    Elongation factor Tu from Escherichia coli cycles between an active conformation where GTP is bound, and an inactive conformation where GDP is bound. Between the two conformations, elongation factor Tu undergoes major structural changes. The aim of this work has been to reveal the role of two very...

  12. The YvfTU two-component system is involved in plcR expression in Bacillus cereus.

    Science.gov (United States)

    Brillard, Julien; Susanna, Kim; Michaud, Caroline; Dargaignaratz, Claire; Gohar, Michel; Nielsen-Leroux, Christina; Ramarao, Nalini; Kolstø, Anne-Brit; Nguyen-the, Christophe; Lereclus, Didier; Broussolle, Véronique

    2008-10-16

    Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative role of YvfTU in the expression of the PlcR regulon was therefore investigated. Expression of the plcR gene was monitored using a transcriptional fusion with a lacZ reporter gene in a yvfTU mutant and in its B. cereus ATCC 14579 parental strain. Two hours after the onset of the stationary phase, a stage at which the PlcR regulon is highly expressed, the plcR expression in the yvfTU mutant was only 50% of that of its parental strain. In addition to the reduced plcR expression in the yvfTU mutant, a few members of the PlcR regulon showed a differential expression, as revealed by transcriptomic and proteomic analyses. The virulence of the yvfTU mutant in a Galleria mellonella insect model was slightly lower than that of the parental strain. The YvfTU two-component system is not required for the expression of most of the virulence factors belonging to the PlcR regulon. However, YvfTU is involved in expression of plcR, a major regulator of virulence in B. cereus.

  13. On the Potentials for Synergy Between COST Action TU1406 and the JCSS

    DEFF Research Database (Denmark)

    Nielsen, Michael Havbro Faber

    On of the main challenges of COST Action TU1406 is to identify, categorize and to the extent possible represent performance indicators of roadway bridges in a manner supporting decision making for their service life integrity management. This calls for an information consistent approach accounting...... Committee to improve the general knowledge on structural safety. The main expertise of the JCSS is centered on the probabilistic modeling of structural performance characteristics of relevance for their safety and efficiency - and aligns well with many of the challenges of COST Action TU1406. The present...... paper thus first provides a short overview of the past and present activities of the JCSS. Thereafter a summary of the some of the key challenges of COST Action 1406 are outlined together with a proposal for their synthetization. Finally, ideas on how the COST Action TU1406 could contribute to the work...

  14. Harmonization of sound insulation descriptors and classification schemes in Europe: COST Action TU0901

    DEFF Research Database (Denmark)

    Rasmussen, Birgit

    -in-Chief. Handbook of noise and vibration control, USA: Wiley and Son; 2007 [Ch. 114]. [4] COST Action TU0901 “Integrating and Harmonizing Sound Insulation Aspects in Sustainable Urban Housing Constructions”, 2009-2013, www.cost.eu/index.php?id=240&action_number=tu0901 (public information at COST website) or http...... Sound Insulation Aspects in Sustainable Urban Housing Constructions" has been approved and runs for four years from November 2009. Until now (end 2010), 28 countries in Europe and 3 overseas countries have signed up for TU0901, and about 85 people have been nominated for the management committee...... of the inhabitants and the society. References [1] "Sound insulation between dwellings – Descriptors in building regulations in Europe" by Birgit Rasmussen & Jens Holger Rindel. Applied Acoustics, 2010, 71(3), 171-180. http://dx.doi.org/10.1016/j.apacoust.2009.05.002 [2] "Sound insulation between dwellings...

  15. Calibration Methods for Air Coupled Antennas - COST Action TU1208

    Science.gov (United States)

    Marecos, Vânia; Solla, Mercedes; Fontul, Simona; Pajewski, Lara

    2016-04-01

    This work focuses on the comparison of different methods for calibrating air coupled antennas: Coring, Surface Reflection Method (SRM) and Common Mid-Point (CMP) through the analysis of GPR data collected in a test site with different pavement solutions. Research activities have been carried out during a Short Term Scientific Mission (STSM) funded by the COST (European Cooperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" in December 2015. The use of GPR in transport infrastructures represents one of the most significant advances for obtaining continuous data along the road, with the advantage of operation at traffic speed and being a non-destructive technique. Its main application has been the evaluation of layer thickness. For the determination of layer thickness, it is necessary to know the velocity of the signal, which depends on the dielectric constant of the material, and the two-way travel time of the reflected signal that is recorded by the GPR system. The calculation of the dielectric value of the materials can be done using different approaches such as: using fixed values based on experience, laboratory determination of dielectric values, applying the SRM, performing back calculation from ground truth references such as cores and test pits, or using the CMP method. The problem with using ground truth is that it is time consuming, labour intensive and intrusive to traffic, in addition, a drill core is not necessarily representative of the whole surveyed area. Regarding the surface reflection technique, one of the problems is that it only measures the dielectric value from the layer surface and not from the whole layer. Recent works already started to address some of these challenges proposing new approaches for GPR layer thickness measurements using multiple antennas to calculate the average dielectric value of the asphalt layer, taking advantage of significant hardware improvements in GPR

  16. I Passiuna tu Christù - Rito e teatro di una cantica popolare della Grecìa Salentina

    Directory of Open Access Journals (Sweden)

    Diana Costa

    2012-01-01

    Full Text Available Abstract – IT Questo articolo si basa sugli esiti di una ricerca di campo condotta nei paesi della Grecìa Salentina,una zona del basso Salento, dove si riscontra una peculiare modalità di esecuzione della cantica popolare grìca conosciuta come I Passiuna tu Christù (La Passione di Cristo. Attorno alla metà degli anni ´90 del ´900 la tradizione della cantica è stata oggetto di un prezioso recupero, fondato sulla memoria e disponibilità di alcuni anziani cantori, che l’ha riportata in vita dopo anni di silenzio. Lo studio si è quindi focalizzato sull'esecuzione di Zollino, uno dei comuni grecanico-salentini, dove opera l'associazione culturale "Bottega del Teatro" che per prima ha promosso il recupero di cui si è detto. Protagonista e interprete raffinato della versione zollinese è l'anziano cantore Antimo Pellegrino, la cui voce si fa teatro attraverso un uso sapiente del corpo fatto di gesti stilizzati, regolati da codici che consentono di comprendere il divenire performativo, ossia il testo in grìco, anche a chi non intende tale idioma. Abstract – EN This article is based on the results of a field research conducted in the countries of the Grecìa Salentina, an area of Salento, where there is a particular execution method of the folksong known as I Passiuna tu Christù (The Passion of the Christ. Around the mid-90s of the 20th century the tradition of this folksong has been the subject of a careful recovery, based on memory and availability of some older singers, who brought it back to life after years of silence. The study then focused on the Zollino’s execution, one of the grecanico-salentino’s common, in which works the cultural association "Bottega del Teatro", the first promoter of this recovery. The old singer Antimo Pellegrino is the player and interpreter of the Zollino’s refined version, whose voice becomes theater through a wise use of body made of stylized gestures, regulated by codes that allow you to

  17. The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu.

    Science.gov (United States)

    Castro-Roa, Daniel; Garcia-Pino, Abel; De Gieter, Steven; van Nuland, Nico A J; Loris, Remy; Zenkin, Nikolay

    2013-12-01

    Fic proteins are ubiquitous in all of the domains of life and have critical roles in multiple cellular processes through AMPylation of (transfer of AMP to) target proteins. Doc from the doc-phd toxin-antitoxin module is a member of the Fic family and inhibits bacterial translation by an unknown mechanism. Here we show that, in contrast to having AMPylating activity, Doc is a new type of kinase that inhibits bacterial translation by phosphorylating the conserved threonine (Thr382) of the translation elongation factor EF-Tu, rendering EF-Tu unable to bind aminoacylated tRNAs. We provide evidence that EF-Tu phosphorylation diverged from AMPylation by antiparallel binding of the NTP relative to the catalytic residues of the conserved Fic catalytic core of Doc. The results bring insights into the mechanism and role of phosphorylation of EF-Tu in bacterial physiology as well as represent an example of the catalytic plasticity of enzymes and a mechanism for the evolution of new enzymatic activities.

  18. CWI and TU Delft at TREC 2013: Contextual Suggestion, Federated Web Search, KBA, and Web Tracks

    NARCIS (Netherlands)

    A. Bellogín Kouki (Alejandro); G.G. Gebremeskel (Gebre); J. He (Jiyin); J.J.P. Lin (Jimmy); A. Said (Alan); T. Samar (Thaer); A.P. de Vries (Arjen); J.B.P. Vuurens (Jeroen)

    2014-01-01

    htmlabstractThis paper provides an overview of the work done at the Centrum Wiskunde & Informatica (CWI) and Delft University of Technology (TU Delft) for different tracks of TREC 2013. We participated in the Contextual Suggestion Track, the Federated Web Search Track, the Knowledge Base

  19. RESULTS OF AIRPLANE TU-204-300 OPERATED BY "VLADIVOSTOK AVIA" COMPANY FUEL FLOW MONITIRING

    Directory of Open Access Journals (Sweden)

    G. E. Maslennikova

    2014-01-01

    Full Text Available This article describes the results obtained from continuous monitoring of fuel flow on the airplanes Tu-204-300, operated by the aircompany "Vladivostok Avia". The reasons for the change in the cost of each copy of airplane and changes in fuel characteristics due to pilots manner of flying.

  20. Všechno už tu bylo. Jenom trochu menší.

    Czech Academy of Sciences Publication Activity Database

    Mikuláš, Radek

    2016-01-01

    Roč. 95, č. 3 (2016), s. 154-157 ISSN 0042-4544 Institutional support: RVO:67985831 Keywords : geology * geological history of the Earth * historical geology * geological eras * Anthropocene * paleontology * ichnology * fossils * trace fossils * fossil animals * sediments Subject RIV: DB - Geology ; Mineralogy http://casopis.vesmir.cz/clanek/vsechno-uz-tu-bylo

  1. Česká literatura a kultura za protektorátu

    Czech Academy of Sciences Publication Activity Database

    Piorecký, Karel

    2017-01-01

    Roč. 65, č. 3 (2017), s. 482-485 ISSN 0009-0468. [Česká literatura a kultura za protektorátu. Ostrava, 24.11.2016-25.11.2016] Institutional support: RVO:68378068 Keywords : Czech literature * 1938-1945 Subject RIV: AJ - Letters, Mass-media, Audiovision

  2. Two axiomatizations of the kernel of TU games: bilateral and converse reduced game properties

    NARCIS (Netherlands)

    Driessen, Theo; Hu, C.-C.

    We provide two axiomatic characterizations of the kernel of TU games by means of both bilateral consistency and converse consistency with respect to two types of two-person reduced games. According to the first type, the worth of any single player in the two-person reduced game is derived from the

  3. Marketing digital. Guía básica para digitalizar tu empresa

    OpenAIRE

    Sánchez Hervás, David

    2016-01-01

    Reseña, para sección Tribuna de adComunica, del libro: Martínez Polo, Josep M.; Martínez Sánchez. Jesús y Parra, M. Concepción (2015). Marketing Digital. Guía básica para digitalizar tu empresa. Barcelona: Editorial UOC.

  4. Manûtu ša Bābili = the Babylonian subdivision of the mina

    NARCIS (Netherlands)

    van der Spek, R.J.

    A new interpretation of the term Manûtu ša Bābili is presented here. It is not the exchange rate between shekels and drachmas, as was generally assumed, but it is the Babylonian subdivision ("counting") of the mina as opposed to the Greek mina. A Babylonian mina counts 30 staters, a Greek mina 25

  5. Mutational analysis of Escherichia coli elongation factor Tu in search of a role for the N-terminal region

    DEFF Research Database (Denmark)

    Mansilla, Francisco; Knudsen, Charlotte Rohde; Laurberg, M

    1998-01-01

    We have mutated lysine 2 and arginine 7 in elongation factor Tu from Escherichia coli separately either to alanine or glutamic acid. The aim of the work was to reveal the possible interactions between the conserved N-terminal part of the molecule, which is rich in basic residues and aminoacyl...... this activity. Furthermore, arginine 7 seems to play a role in regulating the binding of GTP. The three-dimensional structure of the ternary complex, EF-Tu:GTP:Phe-tRNAPhe, involving Thermus aquaticus EF-Tu and yeast Phe-tRNA(Phe), shows that Arg7 is in a position which permits salt bridge formation with Asp284...

  6. Function and structure in phage Qbeta RNA replicase. Association of EF-Tu-Ts with the other enzyme subunits

    DEFF Research Database (Denmark)

    Blumenthal, T; Young, R A; Brown, S

    1976-01-01

    of EF-Tu-Ts from the other enzyme subunits: whereas free EF-Tu-Ts binds GDP independently of salt concentration, this binding by Qbeta replicase is sensitive to high ionic strength and remains so in the presence of Qbeta RNA. Furthermore, RNA does not allow the release of EF-Ts from EF-Tu by GTP...... for one another increases with increasing ionic strength. The enzyme is capable of initiation of RNA synthesis with synthetic templates only when in the low ionic strength conformation. Elongation of initiated polynucleotide chains is not affectedby ionic strength. Addition of Qbeta RNA to the enzyme also...... alters its quaternary structure: the EF-Tu-Ts cannot be covalently attached to the other enzyme subunits with bifunctional cross-linking reagents in the presence of RNA. This conformational change is not influenced by ionic strength. The addition of Qbeta RNA to the enzyme, does not result in the release...

  7. Progress of the COST Action TU1402 on the Quantification of the Value of Structural Health Monitoring

    DEFF Research Database (Denmark)

    Thöns, Sebastian; Limongelli, Maria Pina; Ivankovic, Ana Mandic

    2017-01-01

    This paper summarizes the development of Value of Structural Health Monitoring (SHM) Information analyses and introduces the development, objectives and approaches of the COST Action TU1402 on this topic. SHM research and engineering has been focused on the extraction of loading, degradation...... encompassing the infrastructure and the SHM systems, their functionality and thus require the interaction of several research disciplines. For progressing on these points, a scientific networking and dissemination project namely the COST Action TU1402 has been initiated....

  8. Duplication of Drosophila melanogaster mitochondrial EF-Tu: pre-adaptation to T-arm truncation and exclusion of bulky aminoacyl residues.

    Science.gov (United States)

    Sato, Aya; Suematsu, Takuma; Aihara, Koh-Ki; Kita, Kiyoshi; Suzuki, Tsutomu; Watanabe, Kimitsuna; Ohtsuki, Takashi; Watanabe, Yoh-Ichi

    2017-03-07

    Translation elongation factor Tu (EF-Tu) delivers aminoacyl-tRNA (aa-tRNA) to ribosomes in protein synthesis. EF-Tu generally recognizes aminoacyl moieties and acceptor- and T-stems of aa-tRNAs. However, nematode mitochondrial (mt) tRNAs frequently lack all or part of the T-arm that is recognized by canonical EF-Tu. We previously reported that two distinct EF-Tu species, EF-Tu1 and EF-Tu2, respectively, recognize mt tRNAs lacking T-arms and D-arms in the mitochondria of the chromadorean nematode Caenorhabditis elegans C. elegans EF-Tu2 specifically recognizes the seryl moiety of serylated D-armless tRNAs. Mitochondria of the enoplean nematode Trichinella possess three structural types of tRNAs: T-armless tRNAs, D-armless tRNAs, and cloverleaf tRNAs with a short T-arm. Trichinella mt EF-Tu1 binds to all three types and EF-Tu2 binds only to D-armless Ser-tRNAs, showing an evolutionary intermediate state from canonical EF-Tu to chromadorean nematode (e.g. C. elegans ) EF-Tu species. We report here that two EF-Tu species also participate in Drosophila melanogaster mitochondria. Both D. melanogaster EF-Tu1 and EF-Tu2 bound to cloverleaf and D-armless tRNAs. D. melanogaster EF-Tu1 has the ability to recognize T-armless tRNAs that do not evidently exist in D. melanogaster mitochondria, but do exist in related arthropod species. In addition, D. melanogaster EF-Tu2 preferentially bound to aa-tRNAs carrying small amino acids, but not to aa-tRNAs carrying bulky amino acids. These results suggest that the Drosophila mt translation system could be another intermediate state between the canonical and nematode mitochondria-type translation systems. © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society.

  9. TU-AB-BRC-06: Dose Calculation in Curved Space

    Energy Technology Data Exchange (ETDEWEB)

    Kieselmann, J; Bartzsch, S; Oelfke, U [The Institute of Cancer Research and The Royal Marsden NHS Foundation Trust, London (United Kingdom)

    2016-06-15

    Purpose: Microbeam Radiation Therapy is a preclinical method in radiation oncology that modulates radiation fields on a micrometre scale. Dose calculation is challenging due to arising dose gradients and therapeutically important dose ranges. Monte Carlo (MC) simulations, often used as gold standard, are computationally expensive and hence too slow for the optimisation of treatment parameters in future clinical applications. On the other hand, conventional kernel based dose calculation leads to inaccurate results close to material interfaces. The purpose of this work is to overcome these inaccuracies while keeping computation times low. Methods: A point kernel superposition algorithm is modified to account for tissue inhomogeneities. Instead of conventional ray tracing approaches, methods from differential geometry are applied and the space around the primary photon interaction is locally warped. The performance of this approach is compared to MC simulations and a simple convolution algorithm (CA) for two different phantoms and photon spectra. Results: While peak doses of all dose calculation methods agreed within less than 4% deviations, the proposed approach surpassed a simple convolution algorithm in accuracy by a factor of up to 3 in the scatter dose. In a treatment geometry similar to possible future clinical situations differences between Monte Carlo and the differential geometry algorithm were less than 3%. At the same time the calculation time did not exceed 15 minutes. Conclusion: With the developed method it was possible to improve the dose calculation based on the CA method with respect to accuracy especially at sharp tissue boundaries. While the calculation is more extensive than for the CA method and depends on field size, the typical calculation time for a 20×20 mm{sup 2} field on a 3.4 GHz and 8 GByte RAM processor remained below 15 minutes. Parallelisation and optimisation of the algorithm could lead to further significant calculation time

  10. On the Potentials for Synergy Between COST Action TU1406 and the JCSS

    DEFF Research Database (Denmark)

    Nielsen, Michael Havbro Faber

    On of the main challenges of COST Action TU1406 is to identify, categorize and to the extent possible represent performance indicators of roadway bridges in a manner supporting decision making for their service life integrity management. This calls for an information consistent approach accounting...... for the experience and statistical evidence collected from past performances as well as any relevant models relating to their future performances – with due consideration of associated uncertainties. The Joint Committee on Structural Safety (JCSS) is a pre-normative committee mandated by the in 1971 by the Liaison...... Committee to improve the general knowledge on structural safety. The main expertise of the JCSS is centered on the probabilistic modeling of structural performance characteristics of relevance for their safety and efficiency - and aligns well with many of the challenges of COST Action TU1406. The present...

  11. Impact of seawater [Ca

    NARCIS (Netherlands)

    Mewes, A.; Langer, G.; Thoms, S.; Nehrke, G.; Reichart, G.J.; de Nooijer, L.J.; Bijma, J.

    2015-01-01

    Mg / Ca ratios in foraminiferal tests are routinely used as paleotemperature proxies, but on long timescales, they also hold the potential to reconstruct past seawater Mg / Ca. The impact of both temperature and seawater Mg / Ca on Mg incorporation in Foraminifera has been quantified by a number of

  12. CA-125 blood test

    Science.gov (United States)

    Ovarian cancer - CA-125 test ... CA-125 is a protein that is found more in ovarian cancer cells than in other cells. This ... ovarian cancer . The test is useful if the CA-125 level was high when the cancer was first ...

  13. Geotechnical research on Jiangnan Tu-Dun tomb historical remains in China.

    Science.gov (United States)

    Lu, Jiang; Onitsuka, Katsutada

    2004-03-01

    Geotechnical research on historical remains can provide new data and distinctive viewpoint for research on soil mechanics. Two Jiangnan Tu-Dun tombs historical remains were investigated by means of in situ and laboratory geotechnical tests. In the present paper the geotechnical properties of these man-made earthworks, especially ageing effect on consolidation and direct shear, and compaction properties of earth fill are discussed.

  14. Proteosynthetic elongation factor EF-Tu domains: Thermal stability, adaptation and functions

    Czech Academy of Sciences Publication Activity Database

    Jonák, Jiří; Šanderová, Hana; Hůlková, Marta; Maloň, Petr; Krásný, Libor; Kepková, M.

    2009-01-01

    Roč. 16, 1a (2009), b23-b24 ISSN 1211-5894. [Meeting of the Czechoslovak and Slovak Biologists /7./. 12.03.2009-14.03.2009, Nové Hrady] R&D Projects: GA MŠk 2B06065; GA MZd NR9138 Institutional research plan: CEZ:AV0Z50520514; CEZ:AV0Z40550506 Keywords : EF-Tu * proteosynthetic elongation factor * thermal stability Subject RIV: EB - Genetics ; Molecular Biology

  15. Bacterial elongation factors EF-Tu, their mutants, chimeric forms, and domains: isolation and purification

    Czech Academy of Sciences Publication Activity Database

    Jonák, Jiří

    2007-01-01

    Roč. 849, 1-2 (2007), s. 141-153 ISSN 1570-0232 R&D Projects: GA AV ČR IAA5052206; GA AV ČR KJB500520503; GA MŠk 2B06065 Institutional research plan: CEZ:AV0Z50520514 Keywords : bacterial elongation factors EF-Tu, , G-domain * recombinant EF-Tus * preparation Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.935, year: 2007

  16. Töötu laevamehhaanik õppis seitsme kuuga kokaks / Agne Narusk

    Index Scriptorium Estoniae

    Narusk, Agne, 1971-

    2004-01-01

    Töötu Jevgeni Solovjov õppis OÜ-s Isabella kokaks, ettevõte on üks 54 koolitajast, kellelt Tallinna tööhõiveamet teenust ostab. 2004. aastaks oli Tallinna tööhõiveametil tööotsijate koolitamiseks 7 mln krooni, millest jätkus pooleks aastaks. Lisa: Tööandjad saavad küsitluslehed

  17. A new condensed toluene mechanism for Carbon Bond: CB05-TU

    Science.gov (United States)

    Whitten, Gary Z.; Heo, Gookyoung; Kimura, Yosuke; McDonald-Buller, Elena; Allen, David T.; Carter, William P. L.; Yarwood, Greg

    2010-12-01

    Toluene is ubiquitous in urban atmospheres and is a precursor to tropospheric ozone and aerosol (smog). An important characteristic of toluene chemistry is the tendency of some degradation products (e.g., cresols and methyl-catechols) to form organic nitro and nitrate compounds that sequester NOx (NO and NO 2) from active participation in smog formation. Explaining the NOx sinks in toluene degradation has made mechanism development more difficult for toluene than for many other organic compounds. Another challenge for toluene is explaining sources of radicals early in the degradation process. This paper describes the development of a new condensed toluene mechanism consisting of 26 reactions, and evaluates the performance of CB05 with this new toluene scheme (Toluene Update, TU) against 38 chamber experiments at 7 different environmental chambers, and provides recommendations for future developments. CB05 with the current toluene mechanism (CB05-Base) under-predicted the maximum O 3 and O 3 production rate for many of these toluene-NOx chamber experiments, especially under low-NOx conditions ([NOx] t=0 toluene mechanism (CB05-TU) includes changes to the yields and reactions of cresols and ring-opening products, and showed better performance than CB05-Base in predicting the maximum O 3, O 3 formation rate, NOx removal rate and cresol concentration. Additional environmental chamber simulations with xylene-NOx experiments showed that the TU mechanism updates tended to improve mechanism performance for xylene.

  18. crdi.ca

    International Development Research Centre (IDRC) Digital Library (Canada)

    et des enfants d'Afrique. INITIATIVE CONCERTÉE. Innovation pour la santé des mères et des enfants d'Afrique. Centre de recherches pour le développement international. CP Box 8500 Ottawa ON Canada K1G 3H9. Téléphone : +1 613 236 6163 • Télécopieur : +1 613 657 7749 ismea@crdi.ca | www.crdi.ca/ismea crdi.ca.

  19. INTRACELLULAR Ca2+ HOMEOSTASIS

    Directory of Open Access Journals (Sweden)

    Shahdevi Nandar Kurniawan

    2015-01-01

    Full Text Available Ca2+ signaling functions to regulate many cellular processes. Dynamics of Ca2+ signaling or homeostasis is regulated by the interaction between ON and OFF reactions that control Ca2+ flux in both the plasma membrane and internal organelles such as the endoplasmic reticulum (ER and mitochondria. External stimuli activate the ON reactions, which include Ca2+ into the cytoplasm either through channels in the plasma membrane or from internal storage like in ER. Most of the cells utilize both channels/sources, butthere area few cells using an external or internal source to control certain processes. Most of the Ca2+ entering the cytoplasm adsorbed to the buffer, while a smaller part activate effect or to stimulate cellular processes. Reaction OFF is pumping of cytoplasmic Ca2+ using a combination mechanism of mitochondrial and others. Changes in Ca2+ signal has been detected in various tissues isolated from animals induced into diabetes as well as patients with diabetes. Ca2+ signal interference is also found in sensory neurons of experimental animals with diabetes. Ca2+ signaling is one of the main signaling systems in the cell.

  20. Tööotsija töötuks tunnistamise ning töötu abiraha määramise ja maksmise ajutise juhendi kinnitamise kohta

    Index Scriptorium Estoniae

    1991-01-01

    Kehtib 1. aprillist 1991. Lisatud ajutine juhend: töö vahendamine tööotsijale, töötule, töötu abiraha määramine ja maksmine Lisatud: töötu abiraha arvutamine. Muudatus: RT I 1992 nr. 27 art. 362

  1. Site-directed mutagenesis of Arg58 and Asp86 of elongation factor Tu from Escherichia coli: effects on the GTPase reaction and aminoacyl-tRNA binding

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.

    1996-01-01

    Elongation factor Tu from Escherichia coli was mutated separately at positions Asp86 and Arg58, in order to shed light both on the GTPase mechanism of elongation factor Tu and on the binding of aminoacyl-tRNA. In addition, the binding of guanine nucleotides was investigated by determination...

  2. [Talking about the scene of diet and cooking based on Pao Chu Tu (kitchen picture) of Han dynasty].

    Science.gov (United States)

    Yang, Jin-ping

    2009-01-01

    The Pao Chu Tu (kitchen picture) of Han dynasty stone relief reconstructed the state of dietary cooking of Han dynasty with vivid and authentic picture. The Pao Chu Tu (kitchen picture) of Shandong Zhucheng city depicted ancient enormous and busy cooking scene which involved all kinds of cooking activities such as butcher, bailing, stewing, roast and brewing wine etc, displaying the advanced kitchen ware e.g. kettle, caldron, tripod, oven and complex cooking technology of Han Chinese people such as steaming, boiling, stirring baking and roast etc, reflecting rich dietary categories of Han dynasty. The Pao Chu Tu (kitchen picture) of Han dynasty stone relief provide us precious datas for the purpose of the study of dietary living of Han dynasty.

  3. PIK3CA

    Science.gov (United States)

    Ilic, Nina; Birsoy, Kıvanç; Aguirre, Andrew J; Kory, Nora; Pacold, Michael E; Singh, Shambhavi; Moody, Susan E; DeAngelo, Joseph D; Spardy, Nicole A; Freinkman, Elizaveta; Weir, Barbara A; Tsherniak, Aviad; Cowley, Glenn S; Root, David E; Asara, John M; Vazquez, Francisca; Widlund, Hans R; Sabatini, David M; Hahn, William C

    2017-04-25

    Oncogenic PIK3CA mutations are found in a significant fraction of human cancers, but therapeutic inhibition of PI3K has only shown limited success in clinical trials. To understand how mutant PIK3CA contributes to cancer cell proliferation, we used genome scale loss-of-function screening in a large number of genomically annotated cancer cell lines. As expected, we found that PIK3CA mutant cancer cells require PIK3CA but also require the expression of the TCA cycle enzyme 2-oxoglutarate dehydrogenase (OGDH). To understand the relationship between oncogenic PIK3CA and OGDH function, we interrogated metabolic requirements and found an increased reliance on glucose metabolism to sustain PIK3CA mutant cell proliferation. Functional metabolic studies revealed that OGDH suppression increased levels of the metabolite 2-oxoglutarate (2OG). We found that this increase in 2OG levels, either by OGDH suppression or exogenous 2OG treatment, resulted in aspartate depletion that was specifically manifested as auxotrophy within PIK3CA mutant cells. Reduced levels of aspartate deregulated the malate-aspartate shuttle, which is important for cytoplasmic NAD + regeneration that sustains rapid glucose breakdown through glycolysis. Consequently, because PIK3CA mutant cells exhibit a profound reliance on glucose metabolism, malate-aspartate shuttle deregulation leads to a specific proliferative block due to the inability to maintain NAD + /NADH homeostasis. Together these observations define a precise metabolic vulnerability imposed by a recurrently mutated oncogene.

  4. Transportvaneundersøgelsen - Variabeldeklaration : TU 2006-09, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar

    , eller anden årsag. Undervejs omtales en række vigtige detaljer ved spørgeskemaet, men dette notat har ikke til formål at give en udtømmende beskrivelse af hvilke spørgsmål, der er stillet til hvem og hvornår. Her kan det være til stor nytte at besøge testspørgeskemaet på http://tu2010.dk/dev/starttest.php...

  5. Hágase tu voluntad: Una muestra de virtuosismo periodístico.

    Directory of Open Access Journals (Sweden)

    Simón Espinosa Jalil

    2008-09-01

    Full Text Available Descubrir al ser humano detrás de la fría noticia e intentar ponerse en los zapatos del otro para revelar ese descubrimiento a sus lectores, es el primer mandamiento de un cronista. Se analiza la obra "Hágase tu voluntad" de Germán Caycedo que relata la muerte de Monseñor Alejandro Labaka y de la hermana Inés Arango. En la obra se explora el tema de choque cultural, y narra las distintas historias que conforman la gran tragedia del holocausto de los indígenas amazónicos

  6. H1 - antihistamīna preparātu aprite aptiekās

    OpenAIRE

    Palamarčuka, Nadežda

    2009-01-01

    Maģistra darba mērķis bija noskaidrot, kādi H1-antihistamīna preparāti ir vislabāk pazīstami un tiek visvairāk pirkti aptiekās. Pētījumā tika aptaujāti 100 pacienti un analizēti lieltirgotavas MagnumMedical H1-antihistamīna preparātu tirdzniecības apjomi. Iegūtie rezultāti liecina, ka vislielākais H1-antihistamīna preparātu patēriņš ir pirmās paaudzes preparātiem Tavegyl (klemastīns) un Suprastin (hloropiramīns), jo to iegādei nav nepieciešamas receptes, tie ir salīdzinoši lēti un ir bijuši p...

  7. The "Da Ming Hunyi Tu": Repurposing a Ming Map in Sino-African Diplomacy

    Directory of Open Access Journals (Sweden)

    Alexander Akin

    2016-12-01

    Full Text Available In 2002, an exhibition at South Africa’s parliament included a reproduction of the Da Ming Hunyi Tu (Amalgamated map of the Great Ming, citing it as the earliest world map to depict the entire African continent. As part of its broader efforts to shape a narrative of long-standing and peaceful international relations with Africa, the People’s Republic of China formally presented a replica of this map as a gift to the South African government in conjunction with the exhibition. In official statements and popular media coverage alike, the map was described as evidence of a distinctly Chinese approach to global relations, based on benevolence and mutual respect. In particular, the map was ahistorically intertwined with the legacy of Zheng He’s diplomatic expeditions, which reached the East African coast in the early 1400s. To the cartographic historian, however, the depiction of Africa in the Da Ming Hunyi Tu is clearly derived from non-Chinese sources that predate Zheng He’s expeditions. This article examines the ways in which the map has been divorced from its original context to suit modern needs, exemplifying the deployment of cartography to deflect anxieties about the nature of Chinese economic influence in South Africa.

  8. An Interbacterial NAD(P)+ Glycohydrolase Toxin Requires Elongation Factor Tu for Delivery to Target Cells

    Energy Technology Data Exchange (ETDEWEB)

    Whitney, John C.; Quentin, Dennis; Sawai, Shin; LeRoux, Michele; Harding, Brittany N.; Ledvina, Hannah E.; Tran, Bao Q.; Robinson, Howard; Goo, Young Ah; Goodlett, David R.; Raunser, Stefan; Mougous, Joseph D.

    2015-10-08

    Type VI secretion (T6S) influences the composition of microbial communities by catalyzing the delivery of toxins between adjacent bacterial cells. Here, we demonstrate that a T6S integral membrane toxin from Pseudomonas aeruginosa, Tse6, acts on target cells by degrading the universally essential dinucleotides NAD+ and NADP+. Structural analyses of Tse6 show that it resembles mono-ADP-ribosyltransferase proteins, such as diphtheria toxin, with the exception of a unique loop that both excludes proteinaceous ADP-ribose acceptors and contributes to hydrolysis. We find that entry of Tse6 into target cells requires its binding to an essential housekeeping protein, translation elongation factor Tu (EF-Tu). These proteins participate in a larger assembly that additionally directs toxin export and provides chaperone activity. Visualization of this complex by electron microscopy defines the architecture of a toxin-loaded T6S apparatus and provides mechanistic insight into intercellular membrane protein delivery between bacteria.

  9. Orbital period variations of the eclipsing binaries TU Cnc, VZ Leo, and OS Ori

    Science.gov (United States)

    Khaliullina, A. I.

    2017-10-01

    Variations of the orbital periods of the eclipsing binaries TU Cnc, VZ Leo, and OS Ori are analyzed. Secular period decreases were earlier believed to occur in these systems. It is demonstrated that the period variations of TU Cnc can be represented using the light-time effect corresponding to the orbital motion of the eclipsing binary with a period of 78.6 years around the center ofmass of the triple system, with the mass of the third body being M 3 > 0.82 M ⊙. With the same accuracy, the period variations of VZ Leo and OS Ori can be represented either solely using the light-time effect, or a superposition of a secular period decrease and the light-time effect. For VZ Leo, the period of the long-term orbit is 63.8 years in the former case and 67.9 years in the latter case. Similar masses for the third body are indicated in both cases: M 3 > 0.55 M ⊙ and M 3 > 0.61 M ⊙. For OS Ori, the period of the long-term orbit is 46 years and M 3 > 0.5 M ⊙ in the former case, and the period is 36 years and M 3 > 0.6 M ⊙ in the latter case.

  10. CAED Document Repository

    Science.gov (United States)

    Compliance Assurance and Enforcement Division Document Repository (CAEDDOCRESP) provides internal and external access of Inspection Records, Enforcement Actions, and National Environmental Protection Act (NEPA) documents to all CAED staff. The respository will also include supporting documents, images, etc.

  11. Preventive Effect of TU-100 on a Type-2 Model of Colitis in Mice: Possible Involvement of Enhancing Adrenomedullin in Intestinal Epithelial Cells

    Directory of Open Access Journals (Sweden)

    Atsushi Kaneko

    2013-01-01

    Full Text Available Purpose. Crohn's disease (CD and ulcerative colitis (UC, the two major forms of inflammatory bowel disease (IBD, have histopathologically and immunologically different characteristics. We previously reported that a traditional Japanese medicine, daikenchuto (TU-100, ameliorated a trinitrobenzenesulfonic acid- (TNBS- induced type-1 model colitis exhibiting histopathological features of CD through adrenomedullin (ADM enhancement. Our current aims were to examine whether TU-100 ameliorates a type-2 model colitis that histologically resembles UC and identify the active ingredients. Methods. TU-100 was administered orally to mice with oxazolone- (OXN- induced type-2 model colitis. The morbidity was evaluated by body weight loss and the macroscopic score of colonic lesions. ADM was quantified using an EIA kit. Results. TU-100 prevented weight loss and colon ulceration. ADM production by intestinal epithelial cells was increased by TU-100 addition. Screening to identify active ingredients showed that [6]-shogaol and hydroxy α-sanshool enhanced ADM production. Conclusions. TU-100 exerted a protective effect in OXN-induced type-2 model colitis, indicating that TU-100 may be a beneficial agent for treatment of UC.

  12. The YvfTU Two-component System is involved in plcR expression in Bacillus cereus

    NARCIS (Netherlands)

    Brillard, Julien; Susanna, Kim; Michaud, Caroline; Dargaignaratz, Claire; Gohar, Michel; Nielsen-Leroux, Christina; Ramarao, Nalini; Kolsto, Anne-Brit; Nguyen-The, Christophe; Lereclus, Didier; Broussolle, Veronique

    2008-01-01

    Background: Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative

  13. Mitochondrial translation factors of Trypanosoma brucei: elongation factor-Tu has a unique subdomain that is essential for its function

    Czech Academy of Sciences Publication Activity Database

    Cristodero, M.; Mani, J.; Oeljeklaus, S.; Aeberhard, L.; Hashimi, Hassan; Ramrath, D.J.F.; Lukeš, Julius; Warscheid, B.; Schneider, A.

    2013-01-01

    Roč. 90, č. 4 (2013), s. 744-755 ISSN 0950-382X R&D Projects: GA ČR GAP305/12/2261 Institutional support: RVO:60077344 Keywords : mitochondrial translation * Trypanosoma brucei * EF-Tu Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 5.026, year: 2013

  14. Crosslinking of tRNA containing a long extra arm to elongation factor Tu by trans-diamminedichloroplatinum(II)

    DEFF Research Database (Denmark)

    Rasmussen, Nils-Jørgen; Wikman, Friedrik; Clark, Brian F. C.

    1990-01-01

    A tRNA containing a long extra arm, namely E. coli tRNA1Leu has been crosslinked to elongation factor Tu, with the crosslinking reagent trans-diamminedichloroplatinum(II). The nucleotide involved in the crosslinking was identified to be a guanosine in the variable region at position 47F or 47G....

  15. The surface-associated elongation factor Tu is concealed for antibody binding on viable pneumococci and meningococci

    Czech Academy of Sciences Publication Activity Database

    Kolberg, J.; Hammerschmidt, S.; Frank, R.; Jonák, Jiří; Šanderová, Hana; Aase, A.

    2008-01-01

    Roč. 53, č. 2 (2008), s. 222-230 ISSN 0928-8244 R&D Projects: GA AV ČR KJB500520503 Institutional research plan: CEZ:AV0Z50520514; CEZ:AV0Z50200510 Keywords : Streptococcus pneumoniae * Neisseria meningitidis * elongation factor EF-Tu Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.972, year: 2008

  16. Ca isotopes in refractory inclusions

    International Nuclear Information System (INIS)

    Niederer, F.R.; Papanastassiou, D.A.

    1984-01-01

    We report measurements of the absolute isotope abundance of Ca in Ca-Al-rich inclusions from the Allende and Leoville meteorites. Improved high precision measurements are reported also for 46 Ca. We find that nonlinear isotope effects in Ca are extremely rare in these inclusions. The absence of nonlinear effects in Ca, except for the effects in FUN inclusions, is in sharp contrast to the endemic effects in Ti. One fine-grained inclusion shows an excess of 46 Ca of (7 +- 1) per mille, which is consistent with addition of only 46 Ca or of an exotic (*) component with 46 Ca* approx. 48 Ca*. FUN inclusion EK-1-4-1 shows a small 46 Ca excess of (3.3 +- 1.0) per mille; this confirms that the exotic Ca components in EK-1-4-1 were even more deficient in 46 Ca relative to 48 Ca than is the case for normal Ca. The Ca in the Ca-Al-rich inclusions shows mass dependent isotope fractionation effects which have a range from -3.8 to +6.7 per mille per mass unit difference. This range is a factor of 20 wider than the range previously established for bulk meteorites and for terrestrial and lunar samples. Ca and Mg isotope fractionation effects in the Ca-Al-rich inclusions are common and attributed to kinetic isotope effects. (author)

  17. A guidelines handbook for GPR surveys in tunnels: a COST Action TU1208 contribution

    Science.gov (United States)

    Bianchini Ciampoli, Luca; Alani, Amir M.; Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Tosti, Fabio

    2016-04-01

    A significant open issue concerning the reliability of geophysical methods and in particular of ground penetrating radar (GPR), both in research and professional context, is a general lack of international standards. This is a major problem to be faced, in order to gain scientific strictness for the GPR practices, and to easily extend to the international community the results achieved within the area of single virtuous countries. Producing international guidelines can represent an important step forward, in this sense. In the memorandum of understanding of the COST Action TU1208 is clearly stated that one of the main purposes of the Action is the "development of innovative protocols and guidelines which will be published in a handbook and constitute a basis for European Standards, for an effective GPR application in CE tasks; safety, economic and financial criteria will be integrated within the protocols". Of course this is not a simple task to be accomplished. Firstly, survey procedures are highly dependent on the objective of the survey itself. On the basis of the objective of each geophysical test, the GPR system, the antenna configuration, and even the processing procedures may change. Besides, these procedures are also influenced by the environmental conditions in which the tests are performed. This affects several aspects spanning from hardware to software, but including, for instance, also safety issues. Due to these reasons, one of the main goal of the COST Action TU1208 is the development of several guidelines related to the main applications of GPR in the field of civil engineering. In this work, the structure of a guidelines handbook for GPR activities in tunnels is outlined. In the first sections, the principal references in the field are provided, and the most common GPR equipment and complementary technologies are described. Subsequently, the survey methodologies are explained. Particular attention is paid to the preliminary activities to be carried

  18. Biomarker CA125

    DEFF Research Database (Denmark)

    Kargo, Anette Stolberg

    be detected months before symptoms arise and recurrence is visible on imaging. Therefore, biochemical detection of potential relapse by CA125 assessment can cause significant distress. A decision aid (DA) is a tool that provides information and describes advantages and disadvantages of a specific intervention...... patient organisations and cancer societies. First, a focus group of seven former OC patients was performed followed by a quantitative rating of the DA pilot version. The DA was adapted accordingly and then tested in 14 OC patients with recurrence using a structured interview guide (alpha testing). A final...... agreed that the DA prepared them to make a better decision and helped them reflecting on their concerns regarding CA125. Conclusion: The DA was useful in decision making regarding monitoring CA125 during follow up after OC. The majority of participants indicated that the DA was helpful in clarifying...

  19. “En tu casa o en la mía”: la entrevista como Infoentretenimiento

    Directory of Open Access Journals (Sweden)

    Marián Alonso González

    2016-11-01

    Full Text Available La lucha por la audiencia ha provocado una oferta de contenidos espectacular donde las fronteras de los géneros se han difuminado, dando lugar a nuevos formatos televisivos híbridos que han revitalizado la entrevista como un género de infoentretenimiento. A lo largo de este artículo analizamos el programa “En tu casa o en la mía”, un nuevo formato de entrevista de personalidad que aúna información y entretenimiento con claros elementos cercanos al infoshow. Estudiamos el producto mediático atendiendo a la narrativa, el tratamiento, la estructura y la técnica empleada con el fin de describir los elementos que hacen de este espacio un ejemplo de hibridación de géneros en el contexto televisivo actual.

  20. “En tu casa o en la mía’: the interview as infotainment

    Directory of Open Access Journals (Sweden)

    Marián Alonso González

    2016-11-01

    Full Text Available The fight for viewers has led to a spectacular range of television content on offer where boundaries of genres have disappeared giving rise to new formats of hybrid television programmes. This has given a new lease of life to interviews as a genre of infotainment. The present article analyses the programme ‘En tu casa o en la mía’, a new type of interview of famous personalities which combines information and entertainment with elements that are very similar to the info-show. We shall embark on a study of this media product, its narrative, treatment, structure and the technique used so as to describe the elements that make it an example of gender hybridization in today’s television programmes.

  1. Housing for the poor: Implementation and impact of Tu Casa program in Tijuana

    Directory of Open Access Journals (Sweden)

    Ana Elizabeth Jardón Hernández

    2009-01-01

    Full Text Available This study assesses the operation and some major results of most important federal housing program focus on households in poverty situation, with particular emphasis on their instrumentation process and impacts in Tijuana’s housing problem for poor families. The exam aims to analyses the design changes of the program that forced its transformation from Vivah to Tu Casa, and the operational difficulties for a proper implementation in an urban context, as well as its limited impact for solving popular housing needs. It also seeks to weigh the importance of government subsidies to acquire or improve housing, in their progressive modality, as an alternative for social segments excluded from any other public or private financing option.

  2. Joint efforts to harmonize sound insulation descriptors and classification schemes in Europe (COST TU0901)

    DEFF Research Database (Denmark)

    Rasmussen, Birgit

    2010-01-01

    Sound insulation descriptors, regulatory requirements and classification schemes in Europe represent a high degree of diversity. One implication is very little exchange of experience of housing design and construction details for different levels of sound insulation; another is trade barriers...... for building systems and products. Unfortunately, there is evidence for a development in the "wrong" direction. For example, sound classification schemes for dwellings exist in nine countries. There is no sign on increasing harmonization, rather the contrary, as more countries are preparing proposals with new......, new housing must meet the needs of the people and offer comfort. Also for existing housing, sound insulation aspects should be taken into account, when renovating housing; otherwise the renovation is not “sustainable”. A joint European Action, COST TU0901 "Integrating and Harmonizing Sound Insulation...

  3. Mapping Escherichia coli elongation factor Tu residues involved in binding of aminoacyl-tRNA

    DEFF Research Database (Denmark)

    Wiborg, Ove; Andersen, C; Knudsen, Charlotte Rohde

    1996-01-01

    Two residues of Escherichia coli elongation factor Tu involved in binding of aminoacyl-tRNA were identified and subjected to mutational analysis. Lys-89 and Asn-90 were each replaced by either Ala or Glu. The four single mutants were denoted K89A, K89E, N90A, and N90E, respectively. The mutants......-tRNA, which suggested an important role of Lys-89 and Asn-90 in tRNA binding. Furthermore, our results indicate helix B to be an important target site for nucleotide exchange factor EF-Ts. Also the mutants His-66 to Ala and His-118 to either Ala or Glu were characterized in an in vitro translation assay...

  4. Topological organization of CA3-to-CA1 excitation.

    Science.gov (United States)

    Hongo, Yoshie; Ogawa, Koichi; Takahara, Yuji; Takasu, Keiko; Royer, Sebastien; Hasegawa, Minoru; Sakaguchi, Gaku; Ikegaya, Yuji

    2015-09-01

    The CA1-projecting axons of CA3 pyramidal cells, called Schaffer collaterals, constitute one of the major information flow routes in the hippocampal formation. Recent anatomical studies have revealed the non-random structural connectivity between CA3 and CA1, but little is known regarding the functional connectivity (i.e. how CA3 network activity is functionally transmitted downstream to the CA1 network). Using functional multi-neuron calcium imaging of rat hippocampal slices, we monitored the spatiotemporal patterns of spontaneous CA3 and CA1 burst activity under pharmacological GABAergic blockade. We found that spatially clustered CA3 activity patterns were transformed into layered CA1 activity sequences. Specifically, synchronized bursts initiated from multiple hot spots in CA3 ensembles, and CA1 neurons located deeper in the pyramidal cell layer were recruited during earlier phases of the burst events. The order of these sequential activations was maintained across the bursts, but the sequence velocity varied depending on the inter-burst intervals. Thus, CA3 axons innervate CA1 neurons in a highly topographical fashion. © 2015 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.

  5. Ca2+ current versus Ca2+ channel cooperativity of exocytosis.

    Science.gov (United States)

    Matveev, Victor; Bertram, Richard; Sherman, Arthur

    2009-09-30

    Recently there has been significant interest and progress in the study of spatiotemporal dynamics of Ca(2+) that triggers exocytosis at a fast chemical synapse, which requires understanding the contribution of individual calcium channels to the release of a single vesicle. Experimental protocols provide insight into this question by probing the sensitivity of exocytosis to Ca(2+) influx. While varying extracellular or intracellular Ca(2+) concentration assesses the intrinsic biochemical Ca(2+) cooperativity of neurotransmitter release, varying the number of open Ca(2+) channels using pharmacological channel block or the tail current titration probes the cooperativity between individual Ca(2+) channels in triggering exocytosis. Despite the wide use of these Ca(2+) sensitivity measurements, their interpretation often relies on heuristic arguments. Here we provide a detailed analysis of the Ca(2+) sensitivity measures probed by these experimental protocols, present simple expressions for special cases, and demonstrate the distinction between the Ca(2+) current cooperativity, defined by the relationship between exocytosis rate and the whole-terminal Ca(2+) current magnitude, and the underlying Ca(2+) channel cooperativity, defined as the average number of channels involved in the release of a single vesicle. We find simple algebraic expressions that show that the two are different but linearly related. Further, we use three-dimensional computational modeling of buffered Ca(2+) diffusion to analyze these distinct Ca(2+) cooperativity measures, and demonstrate the role of endogenous Ca(2+) buffers on such measures. We show that buffers can either increase or decrease the Ca(2+) current cooperativity of exocytosis, depending on their concentration and the single-channel Ca(2+) current.

  6. An integrable coupling family of Merola-Ragnisco-Tu lattice systems, its Hamiltonian structure and related nonisospectral integrable lattice family

    International Nuclear Information System (INIS)

    Xu Xixiang

    2010-01-01

    An integrable coupling family of Merola-Ragnisco-Tu lattice systems is derived from a four-by-four matrix spectral problem. The Hamiltonian structure of the resulting integrable coupling family is established by the discrete variational identity. Each lattice system in the resulting integrable coupling family is proved to be integrable discrete Hamiltonian system in Liouville sense. Ultimately, a nonisospectral integrable lattice family associated with the resulting integrable lattice family is constructed through discrete zero curvature representation.

  7. Thermostability of Multidomain Proteins: Elongation Factors EF-Tu from Escherichia coli and Bacillus stearothermophilus and Their Chimeric Forms

    Czech Academy of Sciences Publication Activity Database

    Šanderová, Hana; Hůlková, Marta; Maloň, Petr; Kepková, M.; Jonák, Jiří

    2004-01-01

    Roč. 13, č. 1 (2004), s. 89-99 ISSN 0961-8368 R&D Projects: GA AV ČR IPP1050128; GA ČR GA204/98/0863; GA ČR GA303/02/0689 Institutional research plan: CEZ:AV0Z4055905; CEZ:AV0Z5052915 Keywords : elongation factor EF-Tu, thermostability, chimeric protein Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 4.116, year: 2004

  8. Collaboration of the Dutch research program for radioactive waste disposal (OPERA) and TU Delft

    International Nuclear Information System (INIS)

    Bykov, D.M.; Kloosterman, J.L.

    2015-01-01

    awarded research proposals available at www.covra.nl/downloads/o pera. Building up competences and knowledge on radioactive waste management and geological disposal is an important goal of OPERA. To this end OPERA collaborates with TU Delft to develop an academic curriculum for the chair Chemistry of the Nuclear Fuel Cycle in the Master of Science Engineering. Furthermore, the results obtained in OPERA will be presented at a Summer School which is planned to be organized at the end of the Program. TU Delft educates young specialists, bachelor and master students, by research projects on geological disposal at the new actinide laboratory (U and Th). For such purpose the knowledge transfer from other OPERA partners is also foreseen, including students internships and visits of the research facilities. Reactor Institute Delft (RID) is part of the Applied Sciences faculty of TU Delft and houses the Radiation Science and Technology (RST) department. RID operates the Hoger Onderwijs Reactor (HOR), a 2 MW pool- type research reactor in an academic setting. The reactor is used as a source of neutrons and positrons for research purposes, including those of OPERA. It also provides neutrons to a variety of facilities for radioisotope production and neutron activation analysis. OPERA is financed by the Dutch Ministry of Economic Affairs and the public limited liability company Elektriciteits-Produktiemaatschappij Zuid-Nederland (EPZ) and coordinated by COVRA.

  9. Collaboration of the Dutch research program for radioactive waste disposal (OPERA) and TU Delft

    Energy Technology Data Exchange (ETDEWEB)

    Bykov, D.M.; Kloosterman, J.L. [TU Delft (Netherlands). Reactor Inst. Delft; Neeft, E.A.C.; Verhoef, E.V. [COVRA N.V., Nieuwdorp (Netherlands)

    2015-07-01

    awarded research proposals available at www.covra.nl/downloads/o pera. Building up competences and knowledge on radioactive waste management and geological disposal is an important goal of OPERA. To this end OPERA collaborates with TU Delft to develop an academic curriculum for the chair Chemistry of the Nuclear Fuel Cycle in the Master of Science Engineering. Furthermore, the results obtained in OPERA will be presented at a Summer School which is planned to be organized at the end of the Program. TU Delft educates young specialists, bachelor and master students, by research projects on geological disposal at the new actinide laboratory (U and Th). For such purpose the knowledge transfer from other OPERA partners is also foreseen, including students internships and visits of the research facilities. Reactor Institute Delft (RID) is part of the Applied Sciences faculty of TU Delft and houses the Radiation Science and Technology (RST) department. RID operates the Hoger Onderwijs Reactor (HOR), a 2 MW pool- type research reactor in an academic setting. The reactor is used as a source of neutrons and positrons for research purposes, including those of OPERA. It also provides neutrons to a variety of facilities for radioisotope production and neutron activation analysis. OPERA is financed by the Dutch Ministry of Economic Affairs and the public limited liability company Elektriciteits-Produktiemaatschappij Zuid-Nederland (EPZ) and coordinated by COVRA.

  10. Development of SAP-DoA techniques for GPR data processing within COST Action TU1208

    Science.gov (United States)

    Meschino, Simone; Pajewski, Lara; Marciniak, Marian

    2016-04-01

    This work focuses on the use of Sub-Array Processing (SAP) and Direction of Arrival (DoA) approaches for the processing of Ground-Penetrating Radar data, with the purpose of locating metal scatterers embedded in concrete or buried in the ground. Research activities have been carried out during two Short-Term Scientific Missions (STSMs) funded by the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" in May 2015 and January 2016. In applications involving smart antennas and in the presence of several transmitters operating simultaneously, it is important for a receiving array to be able to estimate the Direction of Arrival (DoA) of the incoming signals, in order to decipher how many emitters are present and predict their positions. A number of methods have been devised for DoA estimation: the MUltiple SIgnal Classification (MUSIC) and Estimation of Signal Parameters via Rotational Invariance Technique (ESPRIT) are amongst the most popular ones [1]. In the scenario considered by us, the electromagnetic sources are the currents induced on metal elements embedded in concrete or buried in the ground. GPR radargrams are processed, to estimate the DoAs of the electric field back-scattered by the sought targets. In order to work in near-field conditions, a sub-array processing (SAP) approach is adopted: the radargram is partitioned in sub-radargrams composed of few A-scans each, the dominant DoA is predicted for each sub-radargram. The estimated angles are triangulated, obtaining a set of crossings with intersections condensed around object locations. This pattern is filtered, in order to remove a noisy background of unwanted crossings, and is processed by applying the statistical procedure described in [2]. We tested our approach on synthetic GPR radargrams, obtained by using the freeware simulator gprMax implementing the Finite-Difference Time-Domain method [3]. In particular, we worked with

  11. CA 125 in ovarian cancer

    NARCIS (Netherlands)

    van der Burg, M. E.; Lammes, F. B.; Verweij, J.

    1992-01-01

    The serum tumour marker CA 125 is useful in the management of ovarian cancer, although it has its limitations. Approximately 85% of the ovarian cancer patients have an increased serum CA 125 at the start of treatment. There is a good correlation between the course of CA 125 and the clinical response

  12. DeitY-TU face database: its design, multiple camera capturing, characteristics, and evaluation

    Science.gov (United States)

    Bhowmik, Mrinal Kanti; Saha, Kankan; Saha, Priya; Bhattacharjee, Debotosh

    2014-10-01

    The development of the latest face databases is providing researchers different and realistic problems that play an important role in the development of efficient algorithms for solving the difficulties during automatic recognition of human faces. This paper presents the creation of a new visual face database, named the Department of Electronics and Information Technology-Tripura University (DeitY-TU) face database. It contains face images of 524 persons belonging to different nontribes and Mongolian tribes of north-east India, with their anthropometric measurements for identification. Database images are captured within a room with controlled variations in illumination, expression, and pose along with variability in age, gender, accessories, make-up, and partial occlusion. Each image contains the combined primary challenges of face recognition, i.e., illumination, expression, and pose. This database also represents some new features: soft biometric traits such as mole, freckle, scar, etc., and facial anthropometric variations that may be helpful for researchers for biometric recognition. It also gives an equivalent study of the existing two-dimensional face image databases. The database has been tested using two baseline algorithms: linear discriminant analysis and principal component analysis, which may be used by other researchers as the control algorithm performance score.

  13. Electromagnetic simulators for Ground Penetrating Radar applications developed in COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Giannopoulos, Antonios; Warren, Craig; Antonijevic, Sinisa; Doric, Vicko; Poljak, Dragan

    2017-04-01

    Founded in 1971, COST (European COoperation in Science and Technology) is the first and widest European framework for the transnational coordination of research activities. It operates through Actions, science and technology networks with a duration of four years. The main objective of the COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (4 April 2013 - 3 October 2017) is to exchange and increase knowledge and experience on Ground-Penetrating Radar (GPR) techniques in civil engineering, whilst promoting in Europe a wider use of this technique. Research activities carried out in TU1208 include all aspects of the GPR technology and methodology: design, realization and testing of radar systems and antennas; development and testing of surveying procedures for the monitoring and inspection of structures; integration of GPR with other non-destructive testing approaches; advancement of electromagnetic-modelling, inversion and data-processing techniques for radargram analysis and interpretation. GPR radargrams often have no resemblance to the subsurface or structures over which the profiles were recorded. Various factors, including the innate design of the survey equipment and the complexity of electromagnetic propagation in composite scenarios, can disguise complex structures recorded on reflection profiles. Electromagnetic simulators can help to understand how target structures get translated into radargrams. They can show the limitations of GPR technique, highlight its capabilities, and support the user in understanding where and in what environment GPR can be effectively used. Furthermore, electromagnetic modelling can aid the choice of the most proper GPR equipment for a survey, facilitate the interpretation of complex datasets and be used for the design of new antennas. Electromagnetic simulators can be employed to produce synthetic radargrams with the purposes of testing new data-processing, imaging and inversion algorithms, or assess

  14. Planeando Tu Vida: sex and family life education: fundamentals of development, implementation, and evaluation.

    Science.gov (United States)

    Pick De Weiss, S; Givaudan, M; Givaudan, S

    1993-01-01

    Misinformation about sexuality, reproduction, and contraception is widespread among Mexican adolescents and existing sex education programs have been limited in both scope and availability. To address this situation, the Instituto Mexicano de Investigacion de Familia y Poblacion (IMIFAP) designed a comprehensive sex education program based on data gathered in a 1986 diagnostic survey of 865 adolescents 12-19 years of age and interviews with 365 pregnant adolescents. As part of this preliminary research, one group of teens was exposed to a traditional sex education course while another participated in a program that used participatory learning techniques and emphasized communication skills, assertiveness training, value clarification, peer support, and decision making processes. The latter, more effective approach served as the basis for design of a course, Planeando Tu Vida. Operational evaluations of this course conducted at completion and four and eight months later indicated significant increases in knowledge about contraception, but no effect on age at first intercourse. On the other hand, adolescent males who took the course before onset of sexual activity were significantly more likely to use contraceptives at first intercourse than those in traditional courses. This finding underscores the importance of early initiation of sex education programs. To date, the curriculum has been used in over 100 public and private schools, reaching more than 30,000 adolescents. IMIFAP has since developed more than 70 additional health education course guides aimed at children from preschool through high school, all of which emphasize a participatory approach to learning.

  15. [Control of Aedes aegypti breeding sites with the program Recicla por tu bienestar in Merida, Mexico].

    Science.gov (United States)

    Barrera-Pérez, Mario A; Pavía-Ruz, Norma; Mendoza-Mezquita, Jorge Eduardo; Torres-Arcila, Nerio; Hernández-Hernández, Ramón; Castro-Gamboa, Francisco; Geded-Moreno, Eduardo; Cohuo-Rodríguez, Azael; Medina-Barreiro, Anuar; Koyoc-Cardeña, Edgar; Gómez-Dantés, Héctor; Kroeger, Axel; Vázquez-Prokopec, Gonzalo; Manrique-Saide, Pablo

    2015-01-01

    To determine the importance of Ae. aegypti breeding-sites in Merida;to evaluate the impact of Recicla por tu bienestar (RxB, a recycling program) on the reduction of breeding sites and the perception of participants. The relative importance for pupae production of the different types of breeding-sites was determined. Pre-and post-RxB entomological surveys were performed in participant neighborhoods to evaluate the impact on total containers and positive breeding-sites. A survey on the perception of participating people about dengue prevention and control and RxB was applied. Buckets/pots and "small diverse items" were the most important breeding-sites. RxB had a significant impact in the reduction of total containers (IRR = 0.74), positive containers (IRR = 0.33) and the risk of a house being positive for Ae. aegypti (OR = 0.41). All the interviewed participants referred RxB as needed and most consider it useful. RxB should be considered as a good practice for the dengue vector control.

  16. Establishment and characterization of a novel uterine carcinosarcoma cell line, TU-ECS-1, with mutations of TP53 and KRAS.

    Science.gov (United States)

    Chiba, Yohei; Sato, Seiya; Itamochi, Hiroaki; Suga, Yasuko; Fukagawa, Tomoyuki; Oumi, Nao; Oishi, Tetsuro; Harada, Tasuku; Sugai, Tamotsu; Sugiyama, Toru

    2017-04-01

    A new human uterine carcinosarcoma (UCS) cell line, TU-ECS-1, was established and characterized. The morphological appearance of the cultured cells was an insular of epithelial-like cells arranged in the form of a jigsaw puzzle and mesenchymal-like cells with a spindle-shaped or fibroblast-like morphology. A relatively high proliferation rate was observed with a doubling time of 18.2 h. The chromosome number ranged from 44 to 49 and had an extra chromosome 12 (trisomy 12). The respective half-maximal inhibitory concentrations of cisplatin, paclitaxel, and doxorubicin were 2.9 µM, 154 nM, and 219 ng/mL, respectively. Mutational analysis revealed that TU-ECS-1 cells have mutations of TP53 in exons 4, 6, and 8 and of KRAS at codon 12 (G12D) in exon 2, which is a mutation hot spot on this gene. Western blot analysis showed that p53 protein was overexpressed in TU-ECS-1 cells. Immunostaining of the cultured cells and in vivo tumors showed that the TU-ECS-1 cells and xenografts were positive for epithelial marker cytokeratin AE1/3 and mesenchymal marker vimentin. These results suggested that TU-ECS-1 cells might have both epithelial and mesenchymal characteristics. This cell line may be useful to study the carcinogenesis of UCS and contribute to the development of novel treatment strategies.

  17. COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar": ongoing research activities and third-year results

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Tosti, Fabio

    2016-04-01

    This work aims at disseminating the ongoing research activities and third-year results of the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar." About 350 experts are participating to the Action, from 28 COST Countries (Austria, Belgium, Croatia, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Ireland, Italy, Latvia, Malta, Macedonia, The Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey, United Kingdom), and from Albania, Armenia, Australia, Colombia, Egypt, Hong Kong, Jordan, Israel, Philippines, Russia, Rwanda, Ukraine, and United States of America. In September 2014, TU1208 has been recognised among the running Actions as "COST Success Story" ("The Cities of Tomorrow: The Challenges of Horizon 2020," September 17-19, 2014, Torino, IT - A COST strategic workshop on the development and needs of the European cities). The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, whilst simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Moreover, the Action is oriented to the following specific objectives and expected deliverables: (i) coordinating European scientists to highlight problems, merits and limits of current GPR systems; (ii) developing innovative protocols and guidelines, which will be published in a handbook and constitute a basis for European standards, for an effective GPR application in civil- engineering tasks; safety, economic and financial criteria will be integrated within the protocols; (iii) integrating competences for the improvement and merging of electromagnetic scattering techniques and of data- processing techniques; this will lead to a novel freeware tool for the localization of

  18. Elongation Factor Tu and Heat Shock Protein 70 Are Membrane-Associated Proteins from Mycoplasma ovipneumoniae Capable of Inducing Strong Immune Response in Mice.

    Science.gov (United States)

    Jiang, Fei; He, Jinyan; Navarro-Alvarez, Nalu; Xu, Jian; Li, Xia; Li, Peng; Wu, Wenxue

    2016-01-01

    Chronic non-progressive pneumonia, a disease that has become a worldwide epidemic has caused considerable loss to sheep industry. Mycoplasma ovipneumoniae (M. ovipneumoniae) is the causative agent of interstitial pneumonia in sheep, goat and bighorn. We here have identified by immunogold and immunoblotting that elongation factor Tu (EF-Tu) and heat shock protein 70 (HSP 70) are membrane-associated proteins on M. ovipneumonaiea. We have evaluated the humoral and cellular immune responses in vivo by immunizing BALB/c mice with both purified recombinant proteins rEF-Tu and rHSP70. The sera of both rEF-Tu and rHSP70 treated BALB/c mice demonstrated increased levels of IgG, IFN-γ, TNF-α, IL-12(p70), IL-4, IL-5 and IL-6. In addition, ELISPOT assay showed significant increase in IFN-γ+ secreting lymphocytes in the rHSP70 group when compared to other groups. Collectively our study reveals that rHSP70 induces a significantly better cellular immune response in mice, and may act as a Th1 cytokine-like adjuvant in immune response induction. Finally, growth inhibition test (GIT) of M. ovipneumoniae strain Y98 showed that sera from rHSP70 or rEF-Tu-immunized mice inhibited in vitro growth of M. ovipneumoniae. Our data strongly suggest that EF-Tu and HSP70 of M. ovipneumoniae are membrane-associated proteins capable of inducing antibody production, and cytokine secretion. Therefore, these two proteins may be potential candidates for vaccine development against M. ovipneumoniae infection in sheep.

  19. Elongation Factor Tu and Heat Shock Protein 70 Are Membrane-Associated Proteins from Mycoplasma ovipneumoniae Capable of Inducing Strong Immune Response in Mice.

    Directory of Open Access Journals (Sweden)

    Fei Jiang

    Full Text Available Chronic non-progressive pneumonia, a disease that has become a worldwide epidemic has caused considerable loss to sheep industry. Mycoplasma ovipneumoniae (M. ovipneumoniae is the causative agent of interstitial pneumonia in sheep, goat and bighorn. We here have identified by immunogold and immunoblotting that elongation factor Tu (EF-Tu and heat shock protein 70 (HSP 70 are membrane-associated proteins on M. ovipneumonaiea. We have evaluated the humoral and cellular immune responses in vivo by immunizing BALB/c mice with both purified recombinant proteins rEF-Tu and rHSP70. The sera of both rEF-Tu and rHSP70 treated BALB/c mice demonstrated increased levels of IgG, IFN-γ, TNF-α, IL-12(p70, IL-4, IL-5 and IL-6. In addition, ELISPOT assay showed significant increase in IFN-γ+ secreting lymphocytes in the rHSP70 group when compared to other groups. Collectively our study reveals that rHSP70 induces a significantly better cellular immune response in mice, and may act as a Th1 cytokine-like adjuvant in immune response induction. Finally, growth inhibition test (GIT of M. ovipneumoniae strain Y98 showed that sera from rHSP70 or rEF-Tu-immunized mice inhibited in vitro growth of M. ovipneumoniae. Our data strongly suggest that EF-Tu and HSP70 of M. ovipneumoniae are membrane-associated proteins capable of inducing antibody production, and cytokine secretion. Therefore, these two proteins may be potential candidates for vaccine development against M. ovipneumoniae infection in sheep.

  20. COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar:" ongoing research activities and mid-term results

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Slob, Evert; Tosti, Fabio

    2015-04-01

    This work aims at presenting the ongoing activities and mid-term results of the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar.' Almost three hundreds experts are participating to the Action, from 28 COST Countries (Austria, Belgium, Croatia, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Ireland, Italy, Latvia, Malta, Macedonia, The Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey, United Kingdom), and from Albania, Armenia, Australia, Egypt, Hong Kong, Jordan, Israel, Philippines, Russia, Rwanda, Ukraine, and United States of America. In September 2014, TU1208 has been praised among the running Actions as 'COST Success Story' ('The Cities of Tomorrow: The Challenges of Horizon 2020,' September 17-19, 2014, Torino, IT - A COST strategic workshop on the development and needs of the European cities). The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, whilst simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Moreover, the Action is oriented to the following specific objectives and expected deliverables: (i) coordinating European scientists to highlight problems, merits and limits of current GPR systems; (ii) developing innovative protocols and guidelines, which will be published in a handbook and constitute a basis for European standards, for an effective GPR application in civil- engineering tasks; safety, economic and financial criteria will be integrated within the protocols; (iii) integrating competences for the improvement and merging of electromagnetic scattering techniques and of data- processing techniques; this will lead to a novel freeware tool for the localization of buried objects

  1. Accessing the nuclear symmetry energy in Ca+Ca collisions

    Directory of Open Access Journals (Sweden)

    Chbihi A.

    2012-07-01

    Full Text Available The status of the analysis of the INDRA-VAMOS experiement performed at GANIL, using the reactions 40,48Ca+40,48Ca reactions at 35AMeV, are presented. Isotopic distributions of fragments produced in multifragmentation events provide information on the importance of the surface term contribution in the symmetry energy by comparison to AMD predictions.

  2. Combination of GPR with other NDT techniques in different fields of application - COST Action TU1208

    Science.gov (United States)

    Solla, Mercedes; Pérez-Gracia, Vega; Fontul, Simona; Santos-Assunçao, Sonia; Kucukdemirci, Melda

    2017-04-01

    common geophysical methods used for archaeological prospection (GPR and magnetometry). Regarding cultural heritage, an example in Barcelona (Spain) of the assessment of masonry structural elements, with embedded metallic targets, is included. Seismic tomography and 3D GPR imaging are applied, both supported with endoscopy. The results highlight the most affected areas of the structure and the existence of corroded metallic elements as consequence of humidity. Finally, two case studies support the importance of combining data in geological applications. Firstly, GPR and Electrical Resistivity Tomography (ERT) were combined for the analysis of the littoral drift and the tidal range affecting the transport of sediments in costal environments, and more particularly in O Adro Beach, in Vigo (Spain) that had been subjected to extension activities during the last decades. Secondly, the combination Multibeam Sonar and GPR data is presented for the study of a lake, which is an abandoned kaolin mine. Thus, it was possible to analyze the column of water in all the extension of the lake, while differentiating layers of lacustrine deposits and kaolin rock formations in subsurface. This work represents a contribution to the COST (European Cooperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar". The authors thank COST for funding the Action TU1208.

  3. Modular programming for tuberculosis control, the "AuTuMN" platform.

    Science.gov (United States)

    Trauer, James McCracken; Ragonnet, Romain; Doan, Tan Nhut; McBryde, Emma Sue

    2017-08-07

    Tuberculosis (TB) is now the world's leading infectious killer and major programmatic advances will be needed if we are to meet the ambitious new End TB Targets. Although mathematical models are powerful tools for TB control, such models must be flexible enough to capture the complexity and heterogeneity of the global TB epidemic. This includes simulating a disease that affects age groups and other risk groups differently, has varying levels of infectiousness depending upon the organ involved and varying outcomes from treatment depending on the drug resistance pattern of the infecting strain. We adopted sound basic principles of software engineering to develop a modular software platform for simulation of TB control interventions ("AuTuMN"). These included object-oriented programming, logical linkage between modules and consistency of code syntax and variable naming. The underlying transmission dynamic model incorporates optional stratification by age, risk group, strain and organ involvement, while our approach to simulating time-variant programmatic parameters better captures the historical progression of the epidemic. An economic model is overlaid upon this epidemiological model which facilitates comparison between new and existing technologies. A "Model runner" module allows for predictions of future disease burden trajectories under alternative scenario situations, as well as uncertainty, automatic calibration, cost-effectiveness and optimisation. The model has now been used to guide TB control strategies across a range of settings and countries, with our modular approach enabling repeated application of the tool without the need for extensive modification for each application. The modular construction of the platform minimises errors, enhances readability and collaboration between multiple programmers and enables rapid adaptation to answer questions in a broad range of contexts without the need for extensive re-programming. Such features are particularly

  4. Bacillus anthracis Prolyl 4-Hydroxylase Interacts with and Modifies Elongation Factor Tu

    Energy Technology Data Exchange (ETDEWEB)

    Schnicker, Nicholas J. [Department; Razzaghi, Mortezaali [Department; Guha Thakurta, Sanjukta [Department; Chakravarthy, Srinivas [Biophysics; Dey, Mishtu [Department

    2017-10-17

    Prolyl hydroxylation is a very common post-translational modification and plays many roles in eukaryotes such as collagen stabilization, hypoxia sensing, and controlling protein transcription and translation. There is a growing body of evidence that suggests that prokaryotes contain prolyl 4-hydroxylases (P4Hs) homologous to the hypoxia-inducible factor (HIF) prolyl hydroxylase domain (PHD) enzymes that act on elongation factor Tu (EFTu) and are likely involved in the regulation of bacterial translation. Recent biochemical and structural studies with a PHD from Pseudomonas putida (PPHD) determined that it forms a complex with EFTu and hydroxylates a prolyl residue of EFTu. Moreover, while animal, plant, and viral P4Hs act on peptidyl proline, most prokaryotic P4Hs have been known to target free l-proline; the exceptions include PPHD and a P4H from Bacillus anthracis (BaP4H) that modifies collagen-like proline-rich peptides. Here we use biophysical and mass spectrometric methods to demonstrate that BaP4H recognizes full-length BaEFTu and a BaEFTu 9-mer peptide for site-specific proline hydroxylation. Using size-exclusion chromatography coupled small-angle X-ray scattering (SEC–SAXS) and binding studies, we determined that BaP4H forms a 1:1 heterodimeric complex with BaEFTu. The SEC–SAXS studies reveal dissociation of BaP4H dimeric subunits upon interaction with BaEFTu. While BaP4H is unusual within bacteria in that it is structurally and functionally similar to the animal PHDs and collagen P4Hs, respectively, this work provides further evidence of its promiscuous substrate recognition. It is possible that the enzyme might have evolved to hydroxylate a universally conserved protein in prokaryotes, similar to the PHDs, and implies a functional role in B. anthracis.

  5. Structure of the E. coli ribosome-EF-Tu complex at cryo-EM.

    Science.gov (United States)

    Fischer, Niels; Neumann, Piotr; Konevega, Andrey L; Bock, Lars V; Ficner, Ralf; Rodnina, Marina V; Stark, Holger

    2015-04-23

    Single particle electron cryomicroscopy (cryo-EM) has recently made significant progress in high-resolution structure determination of macromolecular complexes due to improvements in electron microscopic instrumentation and computational image analysis. However, cryo-EM structures can be highly non-uniform in local resolution and all structures available to date have been limited to resolutions above 3 Å. Here we present the cryo-EM structure of the 70S ribosome from Escherichia coli in complex with elongation factor Tu, aminoacyl-tRNA and the antibiotic kirromycin at 2.65-2.9 Å resolution using spherical aberration (Cs)-corrected cryo-EM. Overall, the cryo-EM reconstruction at 2.9 Å resolution is comparable to the best-resolved X-ray structure of the E. coli 70S ribosome (2.8 Å), but provides more detailed information (2.65 Å) at the functionally important ribosomal core. The cryo-EM map elucidates for the first time the structure of all 35 rRNA modifications in the bacterial ribosome, explaining their roles in fine-tuning ribosome structure and function and modulating the action of antibiotics. We also obtained atomic models for flexible parts of the ribosome such as ribosomal proteins L9 and L31. The refined cryo-EM-based model presents the currently most complete high-resolution structure of the E. coli ribosome, which demonstrates the power of cryo-EM in structure determination of large and dynamic macromolecular complexes.

  6. TU1208 Open Database of Radargrams: The Dataset of the IFSTTAR Geophysical Test Site

    Directory of Open Access Journals (Sweden)

    Xavier Dérobert

    2018-03-01

    Full Text Available This paper aims to present a wide dataset of ground penetrating radar (GPR profiles recorded on a full-size geophysical test site, in Nantes (France. The geophysical test site was conceived to reproduce objects and obstacles commonly met in the urban subsurface, in a completely controlled environment; since the design phase, the site was especially adapted to the context of radar-based techniques. After a detailed description of the test site and its building process, the GPR profiles included in the dataset are presented and commented on. Overall, 67 profiles were recorded along eleven parallel lines crossing the test site in the transverse direction; three pulsed radar systems were used to perform the measurements, manufactured by different producers and equipped with various antennas having central frequencies from 200 MHz to 900 MHz. An archive containing all profiles (raw data is enclosed to this paper as supplementary material. This dataset is the core part of the Open Database of Radargrams initiative of COST (European Cooperation in Science and Technology Action TU1208 “Civil engineering applications of Ground Penetrating Radar”. The idea beyond such initiative is to share with the scientific community a selection of interesting and reliable GPR responses, to enable an effective benchmark for direct and inverse electromagnetic approaches, imaging methods and signal processing algorithms. We hope that the dataset presented in this paper will be enriched by the contributions of further users in the future, who will visit the test site and acquire new data with their GPR systems. Moreover, we hope that the dataset will be made alive by researchers who will perform advanced analyses of the profiles, measure the electromagnetic characteristics of the host materials, contribute with synthetic radargrams obtained by modeling the site with electromagnetic simulators, and more in general share results achieved by applying their techniques on

  7. Civil Engineering Applications of Ground Penetrating Radar: Research Perspectives in COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Slob, Evert; Tosti, Fabio

    2013-04-01

    can be used by GPR operators to identify the signatures generated by uncommon targets or by composite structures. Repeated evaluations of the electromagnetic field scattered by known targets can be performed by a forward solver, in order to estimate - through comparison with measured data - the physics and geometry of the region investigated by the GPR. It is possible to identify three main areas, in the GPR field, that have to be addressed in order to promote the use of this technology in the civil engineering. These are: a) increase of the system sensitivity to enable the usability in a wider range of conditions; b) research novel data processing algorithms/analysis tools for the interpretation of GPR results; c) contribute to the development of new standards and guidelines and to training of end users, that will also help to increase the awareness of operators. In this framework, the COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar", proposed by Lara Pajewski, "Roma Tre" University, Rome, Italy, has been approved in November 2012 and is going to start in April 2013. It is a 4-years ambitious project already involving 17 European Countries (AT, BE, CH, CZ, DE, EL, ES, FI, FR, HR, IT, NL, NO, PL, PT, TR, UK), as well as Australia and U.S.A. The project will be developed within the frame of a unique approach based on the integrated contribution of University researchers, software developers, geophysics experts, Non-Destructive Testing equipment designers and producers, end users from private companies and public agencies. The main objective of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, whilst promoting the effective use of this safe and non-destructive technique in the monitoring of systems. In this interdisciplinary Action, advantages and limitations of GPR will be highlighted, leading to the identification of gaps in knowledge and technology

  8. Overview and comparative study of GPR international standards and guidelines - COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Marciniak, Marian; Benedetto, Andrea; Tosti, Fabio

    2016-04-01

    Ground Penetrating Radar (GPR) can be effectively used for non-destructive testing of composite structures and diagnostics affecting the whole life-cycle of civil engineering works. Nevertheless, few recognised international standards exist in this field and inhomogeneous recommendations are present in different countries. Moreover, the levels of knowledge, awareness and experience regarding the use of GPR in civil engineering vary strongly across different European areas. The COST Action TU1208 is working hard on leveraging these differences, by sharing and disseminating knowledge and experience, as well as by developing guidelines and protocols for a safe and effective use of GPR in civil engineering. GPR users need to know which is the best way to conduct GPR measurements and what the quality level for the results should be. The TU1208 guidelines will ensure a higher efficiency and quality of GPR services and they will constitute a scientific basis for the introduction of European Standards on the application of GPR in civil engineering. The aim of this contribution is to present an in-depth overview and critical analysis of the existing GPR international and national standards and guidelines. The main documents considered in our work are listed and briefly described in the following. Three standards are provided by the American Society for Testing and Materials (ASTM), to guide the GPR use for subsurface investigation, evaluation of asphalt-covered concrete bridge decks, and determination of pavement-layer thickness: 1. ASTM D6432-11, Standard Guide for Using the Surface Ground Penetrating Radar Method for Subsurface Investigation, ASTM International, West Conshohocken, PA, 2011, www.astm.org, DOI: 10.1520/D6432-11. 2. ASTM D6087-08, Standard Test Method for Evaluating Asphalt-Covered Concrete Bridge Decks Using Ground Penetrating Radar, ASTM International, West Conshohocken, PA, 2008, www.astm.org, DOI: 10.1520/D6087-08. 3. ASTM D4748-10, Standard Test Method

  9. El Caín de Quevedo entre exégesis e iconografía

    OpenAIRE

    Martinengo, A. (Alessandro)

    2004-01-01

    El artículo propone el análisis del poema quevediano «Caín, por más bien visto, tu fiereza». Este poema está basado en el pasaje del Génesis, 4, 13-16 y presenta dos temas cuya tradición se va a detallar: el asesinato de Abel con una quijada (de asno según la leyenda más común) y la aceptación del sacrificio de Abel por parte de Dios. Así se demuestran los profundos conocimientos de Quevedo en el campo de la iconografía bíblica y su gran sensibilidad del artista frente al texto revelado. T...

  10. Improving containment mass and energy releases for CONTEMPT-LT/028 TU with RELAP5/MOD3

    International Nuclear Information System (INIS)

    DaSilva, H.C.; Choe, W.G.

    1996-01-01

    In order to obtain boundary conditions for RELAP5/MOD3 best estimate (BE) large break (LB) loss-of-coolant accident (LOCA) calculations, it is necessary to utilize a separate containment analysis code CONTEMPT-LT/028 TU, which in turn accepts mass and energy releases from the RELAP5/MOD3 calculation. When these boundary conditions are obtained, they are observed to be significantly lower than those reported in FSAR containment analyses. This motivates the present study, where RELAP5/MOD3 mass and energy releases are generated using the same assumptions listed in the FSAR containment calculations. Then CONTEMPT-LT/028 TU pressures and temperatures calculated with both sets of mass and energy releases are compared. It is seen that those obtained with the RELAP5/MOD3 input are still significantly lower, indicating a level of conservatism in the FSAR mass and energy releases that is even above that explicitly listed and also incorporated into the RELAP5/MOD3 calculation. An important conclusion from this finding is that Environmental Qualification (EQ) issues requiring containment re-analyses are likely to be easily resolved if new mass and energy releases are calculated with state-of-the-art LOCA codes modeling the entire reactor coolant system, even when conservative assumptions are incorporated

  11. COST Action TU1208 - Working Group 4 - Combined use of GPR and other NDT methods & GPR applications in geosciences

    Science.gov (United States)

    Pajewski, Lara; Solla, Mercedes; Fontul, Simona

    2017-04-01

    This work aims at presenting the main results achieved by Working Group (WG) 4 "Different applications of GPR and other NDT technologies in civil engineering" of the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (www.GPRadar.eu, www.cost.eu). The main objective of the Action TU1208, started in April 2013 and ending in October 2017, is to exchange and increase scientific-technical knowledge and experience of Ground Penetrating Radar (GPR) techniques in civil engineering, whilst promoting in Europe the effective use of this safe non-destructive technique. The Action involves more than 150 Institutions from 28 COST Countries, a Cooperating State, 6 Near Neighbour Countries and 6 International Partner Countries. WG4 deals with the use of GPR outside from the civil engineering area, namely in archaeological prospecting and cultural heritage diagnostics, agriculture and management of water resources, investigation of polluted industrial sites, non-destructive testing of living tree trunks, planetary exploration, demining, localization of people buried under avalanches and debris, and more. Furthermore, this WG studies the integration of GPR with other Non-Destructive Testing (NDT) methods. The most relevant achievements stemming from WG4 will be presented during the 2017 EGU GA. These are: (i) The collection of thorough information on the state-of-the-art, ongoing studies, problems and future research needs on the topics of interest for this WG; (ii) The performance of a plethora of interesting case studies in important sites all over Europe, including well-known historical places such as Stonehenge (United Kingdom), Carnuntum (Austria), the Wawel Cathedral (Cracow, Poland), the Tholos Tomb of Acharnon (Athens, Greece), the Łazienki Royal Palace (Warsaw, Poland), and more; (iii) WG4 contributed to the TU1208 Education Pack, an open educational package conceived to teach GPR in University

  12. Rates for some reactions involving 42Ca and 44Ca

    International Nuclear Information System (INIS)

    Cheng, C.W.; King, J.D.

    1980-01-01

    Ground-state reaction rates have been deduced from recent cross section measurements for the 42 CA(α, n) 45 Ti, 42 Ca(p, γ) 43 Sc, and 44 Ca(p, n) 44 Sc reactions. Comparison of these rates with those calculated from a statistical model of nuclear reactions. (Woosley et al) shows good agreement for the first two, but the 44 Ca(p, n) rate is more than a factor of 2 less than the theoretical prediction. Stellar reaction rates have been derived from the ground-state rates by multiplying the ground-state rates by the ratio of stellar to ground-state rates given by the statistical model. Both ground-state and stellar rates have been represented by analytic functions of the temperature. The role of these reactions in the approach to quasi-equilibrium during explosive silicon burning is discussed

  13. COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar": first-year activities and results

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Slob, Evert; Tosti, Fabio

    2014-05-01

    This work aims at presenting the first-year activities and results of COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar". This Action was launched in April 2013 and will last four years. The principal aim of COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, whilst simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Moreover, the Action is oriented to the following specific objectives and expected deliverables: (i) coordinating European scientists to highlight problems, merits and limits of current GPR systems; (ii) developing innovative protocols and guidelines, which will be published in a handbook and constitute a basis for European standards, for an effective GPR application in civil- engineering tasks; safety, economic and financial criteria will be integrated within the protocols; (iii) integrating competences for the improvement and merging of electromagnetic scattering techniques and of data- processing techniques; this will lead to a novel freeware tool for the localization of buried objects, shape-reconstruction and estimation of geophysical parameters useful for civil engineering needs; (iv) networking for the design, realization and optimization of innovative GPR equipment; (v) comparing GPR with different NDT techniques, such as ultrasonic, radiographic, liquid-penetrant, magnetic-particle, acoustic-emission and eddy-current testing; (vi) comparing GPR technology and methodology used in civil engineering with those used in other fields; (vii) promotion of a more widespread, advanced and efficient use of GPR in civil engineering; and (viii) organization of a high-level modular training program for GPR European users. Four Working Groups (WGs) carry out the research activities. The first WG

  14. Mutation of the conserved Gly94 and Gly126 in elongation factor Tu from Escherichia coli. Elucidation of their structural and functional roles

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Kjaersgård, I V; Wiborg, O

    1995-01-01

    All guanine-nucleotide-binding proteins cycle between an inactive, GDP-bound and an active, GTP-bound conformation whereby they function as molecular switches. Elongation factor Tu from Escherichia coli is used as a model for defining residues important in the switch mechanism. Gly94 and Gly126...

  15. Formulations of the endophytic bacterium Bacillus subtilis Tu-100 suppress Sclerotinia sclerotiorum on oilseed rape and improve plant vigor in field trials conducted at separate locations

    Science.gov (United States)

    Sclerotinia sclerotiorum causes serious yield losses in crops in The People’s Republic of China. Two formulations of oilseed rape seed containing the endophytic bacterium Bacillus subtilis Tu-100 were evaluated for suppression of this pathogen in field trials conducted at two independent locations....

  16. Keeleuuendus ja tõlkimine Noor-Eesti kultuurilise utoopia raames : Villem Ridala tõlgitud "Süütu" näitel / Daniele Monticelli

    Index Scriptorium Estoniae

    Monticelli, Daniele

    2006-01-01

    Keeleuuenduse ja tõlketegevuse mõningastest olulistest seostest Noor-Eesti liikumisega alanud kultuurilise revolutsiooni kontekstis. Lähema lingvistilise vaatluse alla võetakse Gabriele D'Annunzio "L'Innocente" ("Süütu") Villem Ridala tõlkes. Järgneb: Keel ja Kirjandus, 2006, nr. 6, lk. 477-490

  17. Nondestructive tests for railway monitoring. European Experience in COST Action TU1208

    Science.gov (United States)

    Fontul, Simona; Solla, Mercedes; Loizos, Andreas

    2016-04-01

    The railway monitoring is an important issue for a proper maintenance planning. With the increase in loads and travel speed, it is important to be able to diagnose the track defects and to plan the proper maintenance without interfering with the users. Traditionally, the maintenance actions are planned based on the geometric level parameters assessed without contact with the line, at traffic speed, by dedicated inspection vehicles. Nevertheless, the geometric condition of the line does not provide information on the defects causes. In order to complements the information on the causes, geophysics measurements can be performed in a nondestructive way. Among these later methods, Ground Penetrating Radar (GPR) is a quick and effective technique to evaluate infrastructure condition in a continuous manner, replacing or reducing the use of traditional drilling method. GPR application to railways infrastructures, during construction and monitoring phase, is relatively recent. It is based on the measuring of layers thicknesses and detection of structural changes. It also enables the assessment of materials properties that constitute the infrastructure and the evaluation of the different types of defects such as ballast pockets, fouled ballast, poor drainage, subgrade settlement and transitions problems. These deteriorations are generally the causes of vertical deviations in track geometry. Moreover, the development of new GPR systems with higher antenna frequencies, better data acquisition systems, more user friendly software and new algorithms for calculation of materials properties can lead to a regular use of GPR. A resume of the European experience in COST Action TU1208 of the application of GPR for railway monitoring and the measurement interpretation is presented in this paper. Also complementary nondestructive tests and other geophysical methods are referred, together with case studies of their application. The main troubleshooting and the needs for data analysis

  18. Sonoma County, CA, 2013 Lidar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Sonoma County Vegetation Mapping and LiDAR Consortium retained WSI to provide lidar and Orthophoto data and derived products in Sonoma County, CA. A classified LAS...

  19. Moisture evaluation of wood material using GPR with WARR method - COST Action TU1208

    Science.gov (United States)

    Reci, Hamza; Sbart'i, Zoubir Mehdi; Pajewski, Lara; Marciniak, Marian

    2016-04-01

    This work deals with the study of the sensitivity of GPR electromagnetic waves to moisture variation in wood material in relation with the direction of fibers and polarization of Electromagnetic field. The relations between relative permittivity and moisture content and the amplitude attenuation with distance was a target study using the direct waves in Wide Angle Radar Reflection (WARR) configuration. Comparison of results measured with reflected waves and direct waves was of main importance since they have different behavior in relation with moisture variation, due to different path of propagation. This research activity has been carried out during one Short-Term Scientific Missions (STSM) funded by the COST (European Cooperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" in November-December 2015. In context of durability evaluation of construction materials, several studies have been carried out by the I2M team, University of Bordeaux, using direct and reflected waves for the evaluation of water content on concrete and wood materials [1-3]. As related to the wood material there is one study carried out using the reflected waves on wood for different humidity and different wood samples, in all the direction of polarization using GPR technique ground coupled antenna at 1.5 GHz [3]. This work continued with different moisture content in order to study the behavior of direct waves as function of moisture. Results taken from those measurements are compared with them from Fixed Offset (reflected method) with one antenna (1.5GHz or 2.6GHz), realized from the previous studies from the I2M and already published [1-3]. The results taken from this work from the reflected waves, show that the effect of wood anisotropy is significant on the variation of relative permittivity with moisture content on wood sample and that is in good agreement with the previous results [3-6]. As related to the direct waves, a small

  20. Assessment of waterfront location in hardened concrete by GPR within COST Action TU1208

    Science.gov (United States)

    Rodríguez-Abad, Isabel; Klysz, Gilles; Balayssac, Jean Paul; Pajewski, Lara

    2016-04-01

    This work focuses on the analysis of the capability of Ground-Penetrating radar (GPR) technique for evaluating how the water penetrates into concrete samples by means of the assessment of the waterfront advance. Research activities have been carried out during a Short-Term Scientific Missions (STSMs) funded by the COST (European Cooperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" in November 2015. The evaluation of water penetrability is crucial in most building materials, such us concrete, since, water and aggressive chemical agents dissolved therein contribute to the deterioration of the material. A number of techniques have been developed to measure their advance in concrete. Although the most common method for measuring water content is the gravimetric method by observing the change in mass, this method has a large number of disadvantages. In this context, non-destructive techniques as GPR play an interesting role. In particular, the application of GPR in the building materials area is providing very promising and interesting results regarding the building materials characterization and especially concrete deterioration evaluation [1-3]. In addition, recent experimental studies highlight the strong relation between wave propagation parameters (velocity and energy level) and water content advance [4-5]. Water content has a decisive influence on dielectric properties and those might be assessed by the study of the wave properties that are derived by using GPR. Therefore, the waterfront advance will result in a change on wave parameters. In line with this, this research is focused on the development of specific processing algorithms necessary to understand how the water penetrates and how the wave parameters will be affected regarding the location of the antenna in reference to the water absorption direction. For this purpose, concrete samples were manufactured, which after curing (90 days) and oven

  1. A Whitham-Theory Sonic-Boom Analysis of the TU-144 Aircraft at a Mach Number of 2.2

    Science.gov (United States)

    Mack, Robert J.

    1999-01-01

    Officially, the Tu-144 was the first supersonic-cruise, passenger-carrying aircraft to enter commercial service. Design, construction, and testing were carried out by the Soviet Union, flight certification was by the Soviet Union, and the only regular passenger flights were scheduled and flown across the territory of the Soviet Union. Although it was not introduced to international passenger service, there were many significant engineering accomplishments achieved in the design, production, and flight of this aircraft. Development of the aircraft began with a prototype stage. Systematic testing and redesign led to a production aircraft in discrete stages that measurably improved the performance of the aircraft from the starting concept to final aircraft certification. It flew in competition with the English-French Concorde for a short time, but was withdrawn from national commercial service due to a lack of interest by airlines outside the Soviet Union. NASA became interested in the Tu- 144 aircraft when it was offered for use as a flying "testbed" in the study of operating characteristics of a supersonic-cruise commercial airplane. Since it had been in supersonic-cruise service, the Tu- 144 had operational characteris'tics similar to those anticipated in the conceptual aircraft designs being studied by the United States aircraft companies. In addition to the other operational tests being conducted on the Tu-144 aircraft, it was proposed that two sets of sonic-boom pressure signature measurements be made. The first set would be made on the ground, using techniques and devices similar to those in reference I and many other subsequent studies. A second set would be made in the air with an instrumented aircraft flying close under the Tu-144 in supersonic flight. Such in-flight measurements would require pressure gages that were capable of accurately recording the flow-field overpressures generated by the Tu- 144 at relatively close distances under the vehicle

  2. Is the sup(99m)Tc-Thyroid-uptake (TcTU) a valid measure of thyroidal trapping

    International Nuclear Information System (INIS)

    Mahlstedt, J.; Hotze, L.A.; Schwedes, H.G.; Joseph, K.

    1979-01-01

    In 2067 euthyroid goitrous patients living in an iodine deficient area which is under medical service of the university of Marburg/L. (W. Germany) sup(99m)Tc-Thyroid-Uptake as a measure for thyroidal trapping has been evaluated with respect to age and sex dependance. For both sexes there is marked decrease with increasing age, the values in the female sex are always higher than in the male sex. Comparison with the relevant results of ODDIE and coworkers reveals general correspondence, the differences of a more strong age and sex dependance are to expect in a region of iodine deficiency. Thus, the study confirms the validity of TcTU as a measure of thyroidal trapping. (orig.) [de

  3. [Information technologies intended to solve contingencies in the Peruvian subsidized health system affiliation: "Resuelve tu afiliación"].

    Science.gov (United States)

    Villegas-Ortega, José; Loyola-Martínez, César; Santisteban-Romero, Javier; Manchego-Lombardi, Mónica; Lozada-Urbano, Michelle

    2016-01-01

    The National Health Authority (SUSALUD) has developed an online platform, "ReSUelve tu afiliación", with the intent to solve the problems with health service access experienced by Peruvian citizens who hold health insurance policies through institutions that manage health insurance funds (IAFAS). This platform virtually articulates the main IAFAS in Peru, which receives requests from any user requiring an update on his/her affiliation status to be resolved within 24 hours. Nearly 8 months after the implementation of this platform, more than 55 thousand applications have been resolved, thus ensuring timely access to health services under the corresponding user coverage form. As a result, this platform has helped to guarantee citizens' rights to health service access in the face of infringement caused by delays in affiliation processing among the different IAFAS in Peru.

  4. COST Action TU1208 - Working Group 2 - GPR surveying of pavements, bridges, tunnels and buildings; underground utility and void sensing

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Derobert, Xavier; Fontul, Simona; Govedarica, Miro; Gregoire, Colette; Loizos, Andreas; Perez-Gracia, Vega; Plati, Christina; Ristic, Aleksandar; Tosti, Fabio; Van Geem, Carl

    2017-04-01

    This work aims at presenting the main results achieved by Working Group (WG) 2 "GPR surveying of pavements, bridges, tunnels and buildings; underground utility and void sensing" of the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (www.GPRadar.eu, www.cost.eu). The principal goal of the Action, started in April 2013 and ending in October 2017, is to exchange and increase scientific-technical knowledge and experience of Ground Penetrating Radar (GPR) techniques in civil engineering, whilst promoting throughout Europe the effective use of this safe non-destructive technique. The Action involves more than 300 Members from 28 COST Countries, a Cooperating State, 6 Near Neighbour Countries and 6 International Partner Countries. The most interesting achievements of WG2 include: 1. The state of the art on the use of GPR in civil engineering was composed and open issues were identified. The few existing international/national guidelines/protocols for GPR inspection in civil engineering were reviewed and discussed. Academic end-users, private companies and stakeholders presented their point of view and needs. 2. Guidelines for investigating flexible pavement by using GPR were prepared, with particular regard to layer-thickness assessment, moisture-content sensing, pavement-damage detection and classification, and other main GPR-based investigations in pavement engineering. 3. Guidelines for GPR sensing and mapping of underground utilities and voids were prepared, with a main focus on urban areas. 4. Guidelines for GPR assessment of concrete structures, with particular regard to inspections in bridges and tunnels, were prepared. 5. A report was composed, including a series of practical suggestions and very useful information to guide GPR users during building inspection. 6. WG2 Members carried out a plethora of case studies where GPR was used to survey roads, highways, airport runways, car

  5. Effects of Ca antagonists on Ca fluxes in resistance vessels

    International Nuclear Information System (INIS)

    Cauvin, C.; Saida, K.; van Breemen, C.

    1982-01-01

    Researchers have examined contractions and 45 Ca fluxes induced by norepinephrine (NE) and 80 mM potassium (high K) depolarization and their inhibition by dilitazem in rabbit mesenteric resistance vessels. Contraction induced by both NE and high K depended almost completely on extracellular Ca. Dose-response curves for diltiazem inhibition of NE (10(-5) M) and high K contractions showed ED50 values of 1 X 10(-8) and 6 X 10(-7) M, respectively, indicating that the receptor-operated channel (ROC) was more sensitive than the potential-operated channel (POC) to the action of diltiazem. Diltiazem (10(-6) M) was shown to inhibit NE- and 80 mM K-stimulated 45 Ca influx effectively by 87 +/- 15 and 85 +/- 10%, respectively. Comparison of these data to those obtained from aorta suggest that although the sensitivity of the POC is approximately the same in aorta and mesenteric resistance vessels, the sensitivity of the ROC is much greater in the latter. This increased sensitivity is paralleled by a greatly decreased role of intracellular Ca release in NE contraction in mesenteric resistance vessels

  6. Adıyaman’da Yayılış Gösteren Nepeta Cataria L. (Lamiaceae) Türü Üzerinde Morfolojik ve Sistematik Bir Çalışma

    OpenAIRE

    KILIÇ, Ömer

    2013-01-01

    Bu çalışmada Adıyaman ilinde yayılış gösteren N. cataria L. türünün morfolojik özellikleri sistematik açı-dan araştırıldı. Stereo mikroskop ile yapılan morfolojik çalışmalar sonucu türlerin yaprak şekli, yaprak tüy örtüsü,ginekeum, androkeum, korolla, kaliks, tohum ve lamentin tekalara birleşme özellikleri belirlendi ve Türkiye Flo-rası’nda ki özellikleriyle karşılaştırıldı. Ek olarak N. cataria türünün gövde ve yaprak tüy örtüsü, polen ve tohumözellikleri SE...

  7. CA125 in ovarian cancer

    DEFF Research Database (Denmark)

    Duffy, M J; Bonfrer, J M; Kulpa, J

    2005-01-01

    value in the detection of early ovarian cancer. At present, therefore, CA125, either alone or in combination with other modalities, cannot be recommended for screening for ovarian cancer in asymptomatic women outside the context of a randomized controlled trial. Preoperative levels in postmenopausal...

  8. tu ša šarri. La « loi du roi »dans la Babylonie achéménide et séleucide dātu ša šarri, The “Law of the King” in Achaemenid and Seleucid Babylonia

    Directory of Open Access Journals (Sweden)

    Sophie Démare-Lafont

    2009-06-01

    Full Text Available Cet article examine le champ sémantique de l’expression dātu ša šarri, « loi du roi », formée sur un terme d’origine vieux-perse (data. La dizaine d’occurrences d’époques achéménide et séleucide montre que la « loi du roi »désigne un mode d’élaboration de la norme issu de la pratique judiciaire du souverain, progressivement compilée pour former un corps de règles invoquées dans les contrats. Ce procédé de création de la loi et de fabrication des recueils législatifs rappelle celui du rescrit romain.This article investigates the meaning of the formula dātu ša šarri, “law of the king”, using the akkadian dātu based on the old Persian word dāta. The ten Achaemenid and Seleucid occurrences of this formula suggest a process of creation of legal rules comparable to the Roman rescript: the legal standard dātu ša šarri derives from the judicial practice of the king, whose decisions were progressively compiled in order to create a body of regulations which were invoked in contracts.

  9. Los narûs (kudurrus babilónicos del Bronce Final y el Hierro = The Babylonian narû (kudurrētu in Late Bronze and Iron Age

    Directory of Open Access Journals (Sweden)

    Sara Arroyo Cuadra

    2016-11-01

    Full Text Available Los narû babilónicos, conocidos tradicionalmente con el nombre de kudurrētu (en singular kudurru, fueron unos monumentos de piedra que incluían representaciones divinas encarnadas generalmente por sus emblemas y escenas con figuras antropomorfas, junto a un texto administrativo sobre transacciones  de tierras. Estos narû vieron la luz con la Dinastía Casita de Babilonia y dejaron de utilizarse en el período Neo-babilónico (ca. 1400-650 a.C.. La gran mayoría de los ejemplares se ha descubierto en Susa en contextos arqueológicos descontextualizados, pero los ejemplares hallados in situ nos hablan de que su dispersión geográfica original hay que situarla en el territorio de la Baja Mesopotamia. Hemos emprendido una investigación orientada a estudiar el corpus de estos monumentos integrado hasta el momento por 178 ejemplares, adoptando una postura interdisciplinar que incluye el análisis de la información que transmiten sus textos, iconografía, materialidad y contexto arqueológico. Por ello, a lo largo del presente artículo se intentará definir el criterio de adscripción de los ejemplares conocidos a dicho corpus según sus propias inscripciones y los estudios previos de  otros autores. A continuación, se hablará de las circunstancias que pudieron motivar su aparición y de los eventos histórico-políticos que pudieron influir en su evolución, así como del ámbito geográfico en que estuvieron vigentes. Después, se analizará el contenido de sus inscripciones y la simbología de su iconografía para, aportar, finalmente, un estudio comparativo entre textos y símbolos, quedando todo ello plasmado en un apartado final de conclusiones.Babylonian narû, traditionally named kudurrētu, were stone monuments with divine representations (generally through its divine emblems and figurative scenes with anthropomorphic figures, together with an administrative text about a land grant. These narû originated in Kassite Babylon and

  10. Control of ciliary motility by Ca2+: Integration of Ca2+-dependent functions and targets for Ca2+ action

    International Nuclear Information System (INIS)

    Evans, T.C.

    1988-01-01

    To identify functions that regulate Ca 2+ -induced ciliary reversal in Paramecium, mutants defective in terminating depolarization-induced backward swimming were selected. Six independent recessive mutations (k-shy) comprising two complementation groups, k-shyA and k-shyB, were identified. All mutants exhibited prolonged backward swimming in depolarizing solutions. Voltage clamp studies revealed that mutant Ca 2+ current amplitudes were reduced, but could be restored to wild type levels by EGTA injection. The recovery of the mutant Ca 2+ current from Ca 2+ -dependent inactivation, and the decay of the Ca 2+ -dependent K + and Ca 2+ -dependent Na + currents after depolarization were slow in k-shy compared to wild type. To identify protein targets of Ca 2+ action, ciliary proteins that interact with calmodulin (CaM) were characterized. With a 125 I-CaM blot assay, several CaM-binding proteins were identified including axonemal, soluble, and membrane-bound polypeptides. Competitive displacement studies with unlabeled Paramecium CaM, bovine CaM, and troponinC suggested that both protein types bind CaM with high affinity and specificity. To examine the presence of CaM-binding sites in intact axonemes, a filtration binding assay was developed

  11. Ca isotopic fractionation patterns in forest ecosystems

    Science.gov (United States)

    Kurtz, A. C.; Takagi, K.

    2012-12-01

    Calcium stable isotope ratios are an emerging tracer of the biogeochemical cycle of Ca that are just beginning to see significant application to forest ecosystems. The primary source of isotopic fractionation in these systems is discrimination against light Ca during uptake by plant roots. Cycling of vegetation-fractionated Ca establishes isotopically distinct Ca pools within a forest ecosystem. In some systems, the shallow soil exchangeable Ca pool is isotopically heavy relative to Ca inputs. This has been explained by preferential removal of light Ca from the soil. In other systems, the soil exchange pool is isotopically light relative to inputs, which is explained by recycling of plant-fractionated light Ca back into soil. Thus vegetation uptake of light Ca has been called on to account for both isotopically heavy and light Ca in the shallow soil exchange pools. We interpret patterns in ecosystem δ44Ca with the aid of a simple box model of the forest Ca cycle. We suggest that the δ44Ca of exchangeable Ca in the shallow soil pool primarily reflects the relative magnitude of three key fluxes in a forest Ca cycle, 1) the flux of external Ca into the system via weathering or atmospheric deposition, 2) the uptake flux of Ca from soils into the vegetation pool, and 3) the return flux of Ca to shallow soils via remineralization of leaf litter. Two observations that emerge from our model may aid in the application of Ca isotopes to provide insight into the forest Ca cycle. First, regardless of the magnitude of both vegetation Ca uptake and isotopic fractionation, the δ44Ca of the soil exchange pool will equal the input δ44Ca unless the plant uptake and remineralization fluxes are out of balance. A second observation is that the degree to which the shallow soil exchange pool δ44Ca can differ from the input ratio is controlled by the relative rates of biological uptake and external Ca input. Significant differences between soil exchange and input δ44Ca are seen only

  12. Dgp71WD is required for the assembly of the acentrosomal Meiosis I spindle, and is not a general targeting factor for the γ-TuRC

    Directory of Open Access Journals (Sweden)

    Richard F. Reschen

    2012-03-01

    Dgp71WD/Nedd1 proteins are essential for mitotic spindle formation. In human cells, Nedd1 targets γ-tubulin to both centrosomes and spindles, but in other organisms the function of Dgp71WD/Nedd1 is less clear. In Drosophila cells, Dgp71WD plays a major part in targeting γ-tubulin to spindles, but not centrosomes, while in Xenopus egg extracts, Nedd1 acts as a more general microtubule (MT organiser that can function independently of γ-tubulin. The interpretation of these studies, however, is complicated by the fact that some residual Dgp71WD/Nedd1 is likely present in the cells/extracts analysed. Here we generate a Dgp71WD null mutant lacking all but the last 12 nucleotides of coding sequence. The complete loss of Dgp71WD has no quantifiable effect on γ-tubulin or Centrosomin recruitment to the centrosome in larval brain cells. The recruitment of γ-tubulin to spindle MTs, however, is severely impaired, and spindle MT density is reduced in a manner that is indistinguishable from cells lacking Augmin or γ-TuRC function. In contrast, the absence of Dgp71WD leads to defects in the assembly of the acentrosomal female Meiosis I spindle that are more severe than those seen in Augmin or γ-TuRC mutants, indicating that Dgp71WD has additional functions that are independent of these complexes in oocytes. Moreover, the localisation of bicoid RNA during oogenesis, which requires γ-TuRC function, is unperturbed in Dgp71WD120 mutants. Thus, Dgp71WD is not simply a general cofactor required for γ-TuRC and/or Augmin targeting, and it appears to have a crucial role independent of these complexes in the acentrosomal Meiosis I spindle.

  13. GPR applications in civil engineering collaborating with a Company and preparation of material for the educational package to teach in Universities. STSM, COST Action TU1208

    Science.gov (United States)

    Sossa, Viviana; Assunção, Sonia; Pérez, Vega

    2017-04-01

    The STSM, Short-Term Missions, was carried out with the support of Murphy Survey company from Dublin - Ireland, was proposed for to Develop a module "GPR Applications in Archaeology" of the educational pack defined in the different meetings of the Cost Action TU 1208. The employ of GPR is mainly determined by the limits and uncertainties associated to this technique that affects to the measurements and the results. Private companies and end-users are highly interested in known the limits associated to particular cases and the advantages of the methodology compared to other studies. During this STSM, we plan to prepare a field survey, analyzing the possible problems and defining the objectives, and to acquire data to assess the structure. This data will be analyzed and processed in order to determine the limits of the technique in the particular case. The results will be compared to other surveys, in order to determine some clear restrictions that the company need to take into account in further works. Aknowledgments: This work has been partially funded by the EU COST Action TU1208, "Civil Engineering Applications of Ground Penetrating Radar", and it is a contribution to the working group 2.2 tasks in the COST Action TU1208.

  14. SCAT/ASCAT Soil Moisture Data: Enhancements in the TU Wien Method for Soil Moisture Retrieval From ERS and METOP Scatterometer Observations

    Science.gov (United States)

    Naeimi, V.; Wagner, W.; Bartalis, Z.

    2009-05-01

    Active microwave remote sensing observations of the scatterometers onboard the European Remote Sensing (ERS) and METeorological OPerational (METOP) satellites have been proven to be valuable for monitoring surface soil moisture globally using the so-called TU Wien change detection method. The METOP satellite series carrying ASCAT (Advanced Scatteromer) instrument for the next 15 years will ensure the continuity of soil moisture retrieval from scatterometers' data for more than 30 years considering the available ERS-1&2 Scatterometer (SCAT) observations dataset. With the aim of implementing a near real-time system for operational soil moisture remote sensing at EUMETSAT, the Institute of Photogrammetry and Remote Sensing at Vienna University of Technology (TU Wien) has developed an improved soil moisture retrieval algorithm to cope with some of the limitations found in the earlier method. The new algorithm has been implemented on a discrete global grid with 12.5 km quasi-equal grid spacing and includes a correction method to reduce azimuthal anisotropy of backscatter signal, new techniques for calculation of the model parameters and incorporates a comprehensive error modeling. The error analysis provides not only the quality information about the product but also facilitates accurate determination of historically driest/wettest conditions during the retrieval process. Enhancements made in the TU Wien retrieval algorithm result in a more uniform performance of the model and, consequently, a spatially consistent soil moisture product with a better spatial resolution.

  15. Rhymes, games and songs for the family and 4-5 ages children of «Educa a tu hijo» program participation

    Directory of Open Access Journals (Sweden)

    Nancy Darías Ávila

    2016-08-01

    Full Text Available The present work is directed to the community as an essential space of our society, it has the objective to encourage the participation of the family and 4-5 ages children in «Educa a tu hijo» program through a group of rhyme, games and songs activities. The research was developed at «Cuba Libre» popular council motivated for the existence of insufficiencies on the participation of «Educa a tu hijo» program and a poor attendance to the rest of planned activities. There were used a group of theoretical, empirical and mathematic methods to solve the problem and fulfil the objective. Taking into account the obtained information it was designed a high motivated propose, which contains rhyme, games and songs that on the Educa a tu hijo program promoters hands constitutes a way to increase family and children participation. The designed and applied propose has had a great acceptation on the studied population and a positive impact in the participation increasing.

  16. COST Action TU1208 - Working Group 3 - Electromagnetic modelling, inversion, imaging and data-processing techniques for Ground Penetrating Radar

    Science.gov (United States)

    Pajewski, Lara; Giannopoulos, Antonios; Sesnic, Silvestar; Randazzo, Andrea; Lambot, Sébastien; Benedetto, Francesco; Economou, Nikos

    2017-04-01

    This work aims at presenting the main results achieved by Working Group (WG) 3 "Electromagnetic methods for near-field scattering problems by buried structures; data processing techniques" of the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (www.GPRadar.eu, www.cost.eu). The main objective of the Action, started in April 2013 and ending in October 2017, is to exchange and increase scientific-technical knowledge and experience of Ground Penetrating Radar (GPR) techniques in civil engineering, whilst promoting in Europe the effective use of this safe non-destructive technique. The Action involves more than 150 Institutions from 28 COST Countries, a Cooperating State, 6 Near Neighbour Countries and 6 International Partner Countries. Among the most interesting achievements of WG3, we wish to mention the following ones: (i) A new open-source version of the finite-difference time-domain simulator gprMax was developed and released. The new gprMax is written in Python and includes many advanced features such as anisotropic and dispersive-material modelling, building of realistic heterogeneous objects with rough surfaces, built-in libraries of antenna models, optimisation of parameters based on Taguchi's method - and more. (ii) A new freeware CAD was developed and released, for the construction of two-dimensional gprMax models. This tool also includes scripts easing the execution of gprMax on multi-core machines or network of computers and scripts for a basic plotting of gprMax results. (iii) A series of interesting freeware codes were developed will be released by the end of the Action, implementing differential and integral forward-scattering methods, for the solution of simple electromagnetic problems by buried objects. (iv) An open database of synthetic and experimental GPR radargrams was created, in cooperation with WG2. The idea behind this initiative is to give researchers the

  17. COST Action TU1208 - Working Group 1 - Design and realisation of Ground Penetrating Radar equipment for civil engineering applications

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; D'Amico, Sebastiano; Ferrara, Vincenzo; Frezza, Fabrizio; Persico, Raffaele; Tosti, Fabio

    2017-04-01

    This work aims at presenting the main results achieved by Working Group (WG) 1 "Novel Ground Penetrating Radar instrumentation" of the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (www.cost.eu, www.GPRadar.eu). The principal goal of the Action, which started in April 2013 and is ending in October 2017, is to exchange and increase scientific-technical knowledge and experience of Ground Penetrating Radar techniques in civil engineering, whilst promoting throughout Europe the effective use of this safe non-destructive technique. The Action involves more than 300 Members from 28 COST Countries, a Cooperating State, 6 Near Neighbour Countries and 6 International Partner Countries. The most interesting achievements of WG1 include: 1. The state of the art on GPR systems and antennas was composed; merits and limits of current GPR systems in civil engineering applications were highlighted and open issues were identified. 2. The Action investigated the new challenge of inferring mechanical (strength and deformation) properties of flexible pavement from electromagnetic data. A semi-empirical method was developed by an Italian research team and tested over an Italian test site: a good agreement was found between the values measured by using a light falling weight deflectometer (LFWD) and the values estimated by using the proposed semi-empirical method, thereby showing great promises for large-scale mechanical inspections of pavements using GPR. Subsequently, the method was tested on a real scale, on an Italian road in the countryside: again, a good agreement between LFWD and GPR data was achieved. As a third step, the method was tested at larger scale, over three different road sections within the districts of Madrid and Guadalajara, in Spain: GPR surveys were carried out at the speed of traffic for a total of 39 kilometers, approximately; results were collected by using different GPR antennas

  18. Bioavailability of iron multi-amino acid chelate preparation in mice and human duodenal HuTu 80 cells.

    Science.gov (United States)

    Kajarabille, Naroa; Brown, Catriona; Cucliciu, Anamaria; Thapaliya, Gita; Latunde-Dada, Gladys O

    2017-03-01

    Strategies for preventing Fe deficiency include Fe supplementation and Fe fortification of foods. The absorption, metabolism and chemical characteristics of Fe multi-amino acid chelate (IMAAC) are not known. Absorption of IMAAC was compared with FeSO4 in Fe-depleted mice and in vitro chemical studies of the Fe supplement was performed in HuTu 80 cells. Hb repletion study was carried out in Fe-deficient CD1 mice that were fed for 10 d a diet supplemented with ferrous IMAAC or FeSO4. A control group of Fe-replete mice was fed a diet with adequate Fe concentrations throughout the study. Tissues were collected from the mice, and the expression of Fe-related genes was determined by quantitative PCR. Ferric reductase and Fe uptake were evaluated in HuTu 80 cells. Supplementation of the diet with FeSO4 or IMAAC significantly increased Hb levels (P<0·001) in Fe-deficient mice from initial 93·9 (SD 10·8) or 116·2 (SD 9·1) to 191 (SD 0·7) or 200 (SD 0·5) g/l, respectively. Initial and final Hb for the Fe-deficient control group were 87·4 (SD 6·7) and 111 (SD 11·7) g/l, respectively. Furthermore, the liver non-haem Fe of both supplement groups increased significantly (P<0·001). IMAAC was more effective at restoring Fe in the spleen compared with FeSO4 (P<0·005). Gene expression showed the IMAAC supplement absorption is regulated by the body's Fe status as it significantly up-regulated hepcidin (P<0·001) and down-regulated duodenal cytochrome b mRNA (P<0·005), similar to the effects seen with FeSO4. A significant proportion of Fe in IMAAC is reduced by ascorbic acid. Fe absorption in mice and cells was similar for both IMAAC and FeSO4 and both compounds induce and regulate Fe metabolism genes similarly in the maintenance of homeostasis in mice.

  19. TU-CD-303-05: Unveiling Tumor Heterogeneity by Molecular Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Jeraj, R. [University of Wisconsin (United States)

    2015-06-15

    these advances in cancer biology research will give medical physicists a new perspective in daily clinical physics practice and in future radiation therapy technological development. Furthermore, academic medical physics should continue to be an integral part of the multidisciplinary cancer research community, harnessing our newly acquired understanding of radiation effects, and developing novel cost-effective treatment strategies to better combat cancer. Learning Objectives: Understand that localized radiation can lead to non-localized secondary effects such as radiation-induced immune response, bystander effect, and abscopal effect. Understand that the non-localized radiation effects may be harnessed to improve cancer treatment. Learn examples of physics participation in multidisciplinary research to advance cancer biology. Recognize the challenges and possibilities of physics applications in cancer research. Chang: NIH 5RC2CA148487-02 and 1U54CA151652-01 Graves: IDEA award (19IB-0106) from the California Breast Cancer Research Program (CBCRP), and by NIH P01 CA67166.

  20. TU-CD-303-05: Unveiling Tumor Heterogeneity by Molecular Imaging

    International Nuclear Information System (INIS)

    Jeraj, R.

    2015-01-01

    these advances in cancer biology research will give medical physicists a new perspective in daily clinical physics practice and in future radiation therapy technological development. Furthermore, academic medical physics should continue to be an integral part of the multidisciplinary cancer research community, harnessing our newly acquired understanding of radiation effects, and developing novel cost-effective treatment strategies to better combat cancer. Learning Objectives: Understand that localized radiation can lead to non-localized secondary effects such as radiation-induced immune response, bystander effect, and abscopal effect. Understand that the non-localized radiation effects may be harnessed to improve cancer treatment. Learn examples of physics participation in multidisciplinary research to advance cancer biology. Recognize the challenges and possibilities of physics applications in cancer research. Chang: NIH 5RC2CA148487-02 and 1U54CA151652-01 Graves: IDEA award (19IB-0106) from the California Breast Cancer Research Program (CBCRP), and by NIH P01 CA67166

  1. A simple method for the accurate determination of free [Ca] in Ca-EGTA solutions.

    Science.gov (United States)

    Bers, D M

    1982-05-01

    A simple method for the accurate determination of free [Ca] in ethyleneglycol-bis(beta-aminoethylether)-N,N'-tetraacetic acid (EGTA)-buffered Ca solutions is described. This method is useful for calibration of Ca macro- and microelectrodes to low free [Ca] and should improve the reliability of calculated free [Ca] in more complex solutions. Briefly, free [Ca] in Ca-EGTA solutions is measured with a Ca electrode, bound Ca is calculated, and Scatchard and double-reciprocal plots are resolved for the total [EGTA] and the apparent Ca-EGTA association constant (K'Ca) in the solutions used. The free [Ca] is then recalculated using the determined parameters, giving a more accurate knowledge of the free [Ca] in these solutions and providing an accurate calibration curve for the Ca electrode. These solutions can then be used to calibrate other Ca electrodes (e.g., Ca microelectrodes) or the calibrated Ca electrode can be used to measure free [Ca] in solutions containing multiple metal ligands. This method allows determination of free [Ca], K'Ca, and total [EGTA] in the actual solutions used regardless of pH, temperature, or ionic strength. It does not require accurate knowledge of K'Ca or EGTA purity and circumvents many potential errors due to assumption of binding parameters. K'Ca was found to be 2.45 +/- 0.04 X 10(6) M-1 in 100 mM KCl, 10 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid, and 1 mM EGTA at pH 7.00 and 23 degrees C. Total [EGTA] varied with supplier but was always less than quoted.

  2. TU-E-217BCD-11: Evaluating the Performance of a Stationary Digital Breast Tomosynthesis System.

    Science.gov (United States)

    Tucker, A; Gidcumb, E; Shan, J; Qian, X; Sprenger, F; Spronk, D; Zhang, Y; Kennedy, D; Farbizio, T; Ruth, C; Jing, Z; Lu, J; Zhou, O

    2012-06-01

    National Cancer Institute under grant number U54CA119343 and R01CA134598 and the UNC University Cancer Research Fund. Dr. Xin Qian is supported by a fellowship from the Department of Defense under grant number BC087505. © 2012 American Association of Physicists in Medicine.

  3. Control de criaderos de Aedes aegypti con el programa Recicla por tu bienestar en Mérida, México

    Directory of Open Access Journals (Sweden)

    Mario Barrera Pérez

    2015-05-01

    Full Text Available Objetivos. Determinar la importancia de los criaderos de Ae. aegypti en Mérida; evaluar el impacto del programa Recicla or tu bienestar (RxB sobre la presencia/abundancia de éstos y la percepción de los habitantes. Material y métodos. Se calculó la importancia de los criaderos por su productividad pupal. Se realizaron muestreos pre y post RxB en colonias para cuantificar el total de recipientes/criaderos. Se aplicó una encuesta a participantes sobre la percepción sobre RxB en colonias seleccionadas. Resultados. Los botes, cubetas y diversos objetos chicos fueron los criaderos más importantes. RxB tuvo un impacto significativo en la reducción del número de recipientes (IRR=0.74, en los recipientes positivos (IRR=0.33 y en la positividad de las viviendas para Ae. aegypti (OR=0.41. Todos los entrevistados opinaron que RxB es necesario la gran mayoría piensa que es útil. Conclusiones. RxB debe ser considerada una buena práctica para el control del vector del dengue.

  4. Increasing HIV-related knowledge, communication, and testing intentions among Latinos: Protege tu Familia: Hazte la Prueba.

    Science.gov (United States)

    Rios-Ellis, Britt; Espinoza, Lilia; Bird, Mara; Garcia, Melawhy; D'Anna, Laura Hoyt; Bellamy, Laura; Scolari, Rosana

    2010-08-01

    Latinos are less likely to be aware of their HIV seropositivity than African Americans and Whites. 'Protege tu Familia: Hazte la Prueba' is a culturally and linguistically-sensitive HIV/AIDS prevention and testing program targeting Latino families. Using community-based participatory research techniques, Spanish-speaking bicultural community health workers helped develop and then used an educational flip chart and materials to conduct outreach and HIV prevention education in diverse settings. The intervention was created to increase HIV/AIDS-related knowledge, to improve communication regarding sexual risk, and to augment intentions to use condoms and test for HIV. A secondary purpose was to decrease HIV-related stigma by improving knowledge about transmission and reducing homophobia. Participants demonstrated significant increases in HIV knowledge, intention to practice safer sex and communicate sexual risk to partner(s), and intention to test for HIV. Improvements were also found in self-reported comfort levels when interacting with and caring for the HIV positive, thus decreasing HIV/AIDS-related stigma.

  5. Applications of GPR in archaeological prospecting and cultural heritage diagnostics: Research Perspectives in COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Schettini, Giuseppe; Soldovieri, Francesco

    2013-04-01

    Ground Penetrating Radar (GPR) is a safe, non-destructive and non-invasive imaging technique that can be effectively used for advanced inspection of composite structures and for diagnostics affecting the whole life-cycle of civil engineering works. GPR can also be successfully employed in archaeological prospecting and cultural heritage diagnostics. In many Countries, where the archeological patrimony is an outstanding value (as Egypt, Israel, Greece, Central and South America), GPR is usually employed both as a diagnostic tool for the preventive detection of archeological structures and as the most advanced instrument able to prospect geometry and shape of underground valuable sites. However many uncertainties persist, because of several difficulties and ambiguities due to the complexity of the image processing in heterogeneous environment. It is possible to identify three main areas, in GPR field, that have to be addressed in order to promote the use of this technology in archaeological prospecting and cultural heritage diagnostics. These are: a) increase of the system sensitivity to enable the usability in a wider range of conditions, archeological sites are often located in impervious and critical environments; b) research novel data processing algorithms/analysis tools for the interpretation of GPR results; c) contribute to the development of new standards and guidelines and to training of end users, that will also help to increase the awareness of operators. It is also important to further investigate and promote a combined use of GPR with other non-invasive advanced techniques, typically used in the archeological investigation. In this framework, the COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar", proposed by a research team of "Roma Tre" University, Rome, Italy, has been approved in November 2012 and is going to start in April 2013. It is a 4-years ambitious project already involving 17 European Countries (AT, BE, CH, CZ, DE

  6. The nuclear inclusion a (NIa protease of turnip mosaic virus (TuMV cleaves amyloid-β.

    Directory of Open Access Journals (Sweden)

    Hye-Eun Han

    Full Text Available BACKGROUND: The nuclear inclusion a (NIa protease of turnip mosaic virus (TuMV is responsible for the processing of the viral polyprotein into functional proteins. NIa was previously shown to possess a relatively strict substrate specificity with a preference for Val-Xaa-His-Gln↓, with the scissile bond located after Gln. The presence of the same consensus sequence, Val(12-His-His-Gln(15, near the presumptive α-secretase cleavage site of the amyloid-β (Aβ peptide led us to hypothesize that NIa could possess activity against Aβ. METHODOLOGY/PRINCIPAL FINDINGS: Western blotting results showed that oligomeric as well as monomeric forms of Aβ can be degraded by NIa in vitro. The specific cleavage of Aβ was further confirmed by mass spectrometry analysis. NIa was shown to exist predominantly in the cytoplasm as observed by immunofluorescence microscopy. The overexpression of NIa in B103 neuroblastoma cells resulted in a significant reduction in cell death caused by both intracellularly generated and exogenously added Aβ. Moreover, lentiviral-mediated expression of NIa in APP(sw/PS1 transgenic mice significantly reduced the levels of Aβ and plaques in the brain. CONCLUSIONS/SIGNIFICANCE: These results indicate that the degradation of Aβ in the cytoplasm could be a novel strategy to control the levels of Aβ, plaque formation, and the associated cell death.

  7. Distinct Roles for Dorsal CA3 and CA1 in Memory for Sequential Nonspatial Events

    Science.gov (United States)

    Farovik, Anja; Dupont, Laura M.; Eichenbaum, Howard

    2010-01-01

    Previous studies have suggested that dorsal hippocampal areas CA3 and CA1 are both involved in representing sequences of events that compose unique episodes. However, it is uncertain whether the contribution of CA3 is restricted to spatial information, and it is unclear whether CA1 encodes order per se or contributes by an active maintenance of…

  8. 46 CFR 7.130 - Point Conception, CA to Point Sur, CA.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Point Conception, CA to Point Sur, CA. 7.130 Section 7.130 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY PROCEDURES APPLICABLE TO THE PUBLIC BOUNDARY LINES Pacific Coast § 7.130 Point Conception, CA to Point Sur, CA. (a) A line drawn from the...

  9. 46 CFR 7.125 - Point Vincente, CA to Point Conception, CA.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Point Vincente, CA to Point Conception, CA. 7.125 Section 7.125 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY PROCEDURES APPLICABLE TO THE PUBLIC BOUNDARY LINES Pacific Coast § 7.125 Point Vincente, CA to Point Conception, CA. (a) A line drawn from...

  10. Multiple Ca2+ sensors in secretion

    DEFF Research Database (Denmark)

    Walter, Alexander M; Groffen, Alexander J; Sørensen, Jakob Balslev

    2011-01-01

    Regulated neurotransmitter secretion depends on Ca(2+) sensors, C2 domain proteins that associate with phospholipids and soluble N-ethylmaleimide-sensitive fusion attachment protein receptor (SNARE) complexes to trigger release upon Ca(2+) binding. Ca(2+) sensors are thought to prevent spontaneous...... fusion at rest (clamping) and to promote fusion upon Ca(2+) activation. At least eight, often coexpressed, Ca(2+) sensors have been identified in mammals. Accumulating evidence suggests that multiple Ca(2+) sensors interact, rather than work autonomously, to produce the complex secretory response...... observed in neurons and secretory cells. In this review, we present several working models to describe how different sensors might be arranged to mediate synchronous, asynchronous and spontaneous neurotransmitter release. We discuss the scenario that different Ca(2+) sensors typically act on one shared...

  11. Direct mobilisation of lysosomal Ca2+ triggers complex Ca2+ signals.

    Science.gov (United States)

    Kilpatrick, Bethan S; Eden, Emily R; Schapira, Anthony H; Futter, Clare E; Patel, Sandip

    2013-01-01

    Accumulating evidence implicates acidic organelles of the endolysosomal system as mobilisable stores of Ca(2+) but their relationship to the better-characterised endoplasmic reticulum (ER) Ca(2+) store remains unclear. Here we show that rapid osmotic permeabilisation of lysosomes evokes prolonged, spatiotemporally complex Ca(2+) signals in primary cultured human fibroblasts. These Ca(2+) signals comprised an initial response that correlated with lysosomal disruption and secondary long-lasting spatially heterogeneous Ca(2+) oscillations that required ER-localised inositol trisphosphate receptors. Electron microscopy identified extensive membrane contact sites between lysosomes and the ER. Mobilisation of lysosomal Ca(2+) stores is thus sufficient to evoke ER-dependent Ca(2+) release probably through lysosome-ER membrane contact sites, and akin to the proposed mechanism of action of the Ca(2+) mobilising messenger nicotinic acid adenine dinucleotide phosphate (NAADP). Our data identify functional and physical association of discrete Ca(2+) stores important for the genesis of Ca(2+) signal complexity.

  12. TU-E-BRA-07: Post-Operative Eye Plaque Imaging Using Tomotherapy MVCT.

    Science.gov (United States)

    Lamb, J; Chao, E; Kamrava, M; Demanes, J; McCannel, T; Low, D

    2012-06-01

    Intra-operative ultrasound is used to verify the positioning of episcleral eye plaques used to treat ocular melanoma. Ultrasound can be ambiguous because of image artifacts, and plaques may shift position after surgery. Ultrasound verification is particularly challenging for anterior tumors. Post-operative imaging could be used to trigger interventions that would prevent local treatment failure. We investigated if, and under what conditions, the Tomotherapy megavoltage computed tomography (MVCT) system could be used to perform post-implantation verification of eye plaque positioning. Plaques were placed on a preserved cow's eye, and imaged with the megavoltage CT of a Tomotherapy linear accelerator (Accuray, Sunnyvale, CA). The images were visually and quantitatively assessed to determine if they were of sufficient quality to verify tumor coverage and plaque tilt with respect to the sclera. We used the visibility of the lens as a proxy for visibility of a tumor. To test the utility of hypothetical higher beam current Tomotherapy images, we averaged sequential images of the same setup. The plaque, the lens of the eye, and the globe are visible in the images. The CNR of the lens with respect to the vitreous was 5.6 for a single image. For 10 images averaged, the CNR was 9.2. Estimated dose from a single image was 1.3 cGy (body CTDIvol); even 10 times this dose would be an acceptable image-guidance dose for radiotherapy patients. One limitation of the imaging procedure is the long scan time (up to 240 seconds), during which time any significant patient motion would lead to image artifacts. Human trials on eye plaque patients are planned. Tomotherapy MVCT imaging could be used to verify tumor coverage and plaque tilt after episcleral plaque implantation. Tumors should be visible in standard Tomotherapy images but higher beam current images would be preferred if available. © 2012 American Association of Physicists in Medicine.

  13. TU-G-210-00: Treatment Planning Strategies, Modeling, Control

    International Nuclear Information System (INIS)

    2015-01-01

    Modeling can play a vital role in predicting, optimizing and analyzing the results of therapeutic ultrasound treatments. Simulating the propagating acoustic beam in various targeted regions of the body allows for the prediction of the resulting power deposition and temperature profiles. In this session we will apply various modeling approaches to breast, abdominal organ and brain treatments. Of particular interest is the effectiveness of procedures for correcting for phase aberrations caused by intervening irregular tissues, such as the skull in transcranial applications or inhomogeneous breast tissues. Also described are methods to compensate for motion in targeted abdominal organs such as the liver or kidney. Douglas Christensen – Modeling for Breast and Brain HIFU Treatment Planning Tobias Preusser – TRANS-FUSIMO – An Integrative Approach to Model-Based Treatment Planning of Liver FUS Tobias Preusser – TRANS-FUSIMO – An Integrative Approach to Model-Based Treatment Planning of Liver FUS Learning Objectives: Understand the role of acoustic beam modeling for predicting the effectiveness of therapeutic ultrasound treatments. Apply acoustic modeling to specific breast, liver, kidney and transcranial anatomies. Determine how to obtain appropriate acoustic modeling parameters from clinical images. Understand the separate role of absorption and scattering in energy delivery to tissues. See how organ motion can be compensated for in ultrasound therapies. Compare simulated data with clinical temperature measurements in transcranial applications. Supported by NIH R01 HL172787 and R01 EB013433 (DC); EU Seventh Framework Programme (FP7/2007-2013) under 270186 (FUSIMO) and 611889 (TRANS-FUSIMO)(TP); and P01 CA159992, GE, FUSF and InSightec (UV)

  14. TU-G-210-01: Modeling for Breast and Brain HIFU Treatment Planning

    International Nuclear Information System (INIS)

    Christensen, D.

    2015-01-01

    Modeling can play a vital role in predicting, optimizing and analyzing the results of therapeutic ultrasound treatments. Simulating the propagating acoustic beam in various targeted regions of the body allows for the prediction of the resulting power deposition and temperature profiles. In this session we will apply various modeling approaches to breast, abdominal organ and brain treatments. Of particular interest is the effectiveness of procedures for correcting for phase aberrations caused by intervening irregular tissues, such as the skull in transcranial applications or inhomogeneous breast tissues. Also described are methods to compensate for motion in targeted abdominal organs such as the liver or kidney. Douglas Christensen – Modeling for Breast and Brain HIFU Treatment Planning Tobias Preusser – TRANS-FUSIMO – An Integrative Approach to Model-Based Treatment Planning of Liver FUS Tobias Preusser – TRANS-FUSIMO – An Integrative Approach to Model-Based Treatment Planning of Liver FUS Learning Objectives: Understand the role of acoustic beam modeling for predicting the effectiveness of therapeutic ultrasound treatments. Apply acoustic modeling to specific breast, liver, kidney and transcranial anatomies. Determine how to obtain appropriate acoustic modeling parameters from clinical images. Understand the separate role of absorption and scattering in energy delivery to tissues. See how organ motion can be compensated for in ultrasound therapies. Compare simulated data with clinical temperature measurements in transcranial applications. Supported by NIH R01 HL172787 and R01 EB013433 (DC); EU Seventh Framework Programme (FP7/2007-2013) under 270186 (FUSIMO) and 611889 (TRANS-FUSIMO)(TP); and P01 CA159992, GE, FUSF and InSightec (UV)

  15. TU-EF-304-08: LET-Based Inverse Planning for IMPT

    Energy Technology Data Exchange (ETDEWEB)

    Gorissen, BL; Giantsoudi, D; Unkelbach, J; Paganetti, H [Massachusetts General Hospital and Harvard Medical School, Boston, MA (United States)

    2015-06-15

    Purpose: Cell survival experiments suggest that the relative biological effectiveness (RBE) of proton beams depends on linear energy transfer (LET), leading to higher RBE near the end of range. With intensity-modulated proton therapy (IMPT), multiple treatment plans that differ in the dose contribution per field may yield a similar physical dose distribution, but the RBE-weighted dose distribution may be disparate. RBE models currently do not have the required predictive power to be included in an optimization model due to the variations in experimental data. We propose an LET-based planning method that guides IMPT optimization models towards plans with reduced RBE-weighted dose in surrounding organs at risk (OARs) compared to inverse planning based on physical dose alone. Methods: Optimization models for physical dose are extended with a term for dose times LET (doseLET). Monte Carlo code is used to generate the physical dose and doseLET distribution of each individual pencil beam. The method is demonstrated for an atypical meningioma patient where the target volume abuts the brainstem and partially overlaps with the optic nerve. Results: A reference plan optimized based on physical dose alone yields high doseLET values in parts of the brainstem and optic nerve. Minimizing doseLET in these critical structures as an additional planning goal reduces the risk of high RBE-weighted dose. The resulting treatment plan avoids the distal fall-off of the Bragg peaks for shaping the dose distribution in front of critical stuctures. The maximum dose in the OARs evaluated with RBE models from literature is reduced by 8–14\\% with our method compared to conventional planning. Conclusion: LET-based inverse planning for IMPT offers the ability to reduce the RBE-weighted dose in OARs without sacrificing target dose. This project was in part supported by NCI - U19 CA 21239.

  16. TU-G-210-01: Modeling for Breast and Brain HIFU Treatment Planning

    Energy Technology Data Exchange (ETDEWEB)

    Christensen, D. [University of Utah (United States)

    2015-06-15

    Modeling can play a vital role in predicting, optimizing and analyzing the results of therapeutic ultrasound treatments. Simulating the propagating acoustic beam in various targeted regions of the body allows for the prediction of the resulting power deposition and temperature profiles. In this session we will apply various modeling approaches to breast, abdominal organ and brain treatments. Of particular interest is the effectiveness of procedures for correcting for phase aberrations caused by intervening irregular tissues, such as the skull in transcranial applications or inhomogeneous breast tissues. Also described are methods to compensate for motion in targeted abdominal organs such as the liver or kidney. Douglas Christensen – Modeling for Breast and Brain HIFU Treatment Planning Tobias Preusser – TRANS-FUSIMO – An Integrative Approach to Model-Based Treatment Planning of Liver FUS Tobias Preusser – TRANS-FUSIMO – An Integrative Approach to Model-Based Treatment Planning of Liver FUS Learning Objectives: Understand the role of acoustic beam modeling for predicting the effectiveness of therapeutic ultrasound treatments. Apply acoustic modeling to specific breast, liver, kidney and transcranial anatomies. Determine how to obtain appropriate acoustic modeling parameters from clinical images. Understand the separate role of absorption and scattering in energy delivery to tissues. See how organ motion can be compensated for in ultrasound therapies. Compare simulated data with clinical temperature measurements in transcranial applications. Supported by NIH R01 HL172787 and R01 EB013433 (DC); EU Seventh Framework Programme (FP7/2007-2013) under 270186 (FUSIMO) and 611889 (TRANS-FUSIMO)(TP); and P01 CA159992, GE, FUSF and InSightec (UV)

  17. TU-G-210-00: Treatment Planning Strategies, Modeling, Control

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-06-15

    Modeling can play a vital role in predicting, optimizing and analyzing the results of therapeutic ultrasound treatments. Simulating the propagating acoustic beam in various targeted regions of the body allows for the prediction of the resulting power deposition and temperature profiles. In this session we will apply various modeling approaches to breast, abdominal organ and brain treatments. Of particular interest is the effectiveness of procedures for correcting for phase aberrations caused by intervening irregular tissues, such as the skull in transcranial applications or inhomogeneous breast tissues. Also described are methods to compensate for motion in targeted abdominal organs such as the liver or kidney. Douglas Christensen – Modeling for Breast and Brain HIFU Treatment Planning Tobias Preusser – TRANS-FUSIMO – An Integrative Approach to Model-Based Treatment Planning of Liver FUS Tobias Preusser – TRANS-FUSIMO – An Integrative Approach to Model-Based Treatment Planning of Liver FUS Learning Objectives: Understand the role of acoustic beam modeling for predicting the effectiveness of therapeutic ultrasound treatments. Apply acoustic modeling to specific breast, liver, kidney and transcranial anatomies. Determine how to obtain appropriate acoustic modeling parameters from clinical images. Understand the separate role of absorption and scattering in energy delivery to tissues. See how organ motion can be compensated for in ultrasound therapies. Compare simulated data with clinical temperature measurements in transcranial applications. Supported by NIH R01 HL172787 and R01 EB013433 (DC); EU Seventh Framework Programme (FP7/2007-2013) under 270186 (FUSIMO) and 611889 (TRANS-FUSIMO)(TP); and P01 CA159992, GE, FUSF and InSightec (UV)

  18. Appccounting: gestiona tu microempresa desde tu Smartphone

    OpenAIRE

    MARTÍNEZ GONZÁLEZ, FABIÁN

    2016-01-01

    [ES] El presente Trabajo de Fin de Grado consiste en la creación de una aplicación para sistemas operativos Android. El trabajo se plantea como el desarrollo de la aplicación en sí y como un proyecto de negocio. Dada la situación actual económica de España y el elevado número de micro-empresas existentes, hay una buena oportunidad de negocio a largo plazo proporcionando un pequeño sistema de información que una micro-empresa se pueda permitir y le permita crecer con el uso de l...

  19. Ca2+/cation antiporters (CaCA: Identification, characterization and expression profiling in bread wheat (Triticum aestivum L.

    Directory of Open Access Journals (Sweden)

    Mehak Taneja

    2016-11-01

    Full Text Available The Ca2+/cation antiporters (CaCA superfamily proteins play vital function in Ca2+ ion homeostasis, which is an important event during development and defense response. Molecular characterization of these proteins has been performed in certain plants, but they are still not characterized in Triticum aestivum (bread wheat. Herein, we identified thirty four TaCaCA superfamily proteins, which were classified into TaCAX, TaCCX, TaNCL and TaMHX protein families based on their structural organization and evolutionary relation with earlier reported proteins. Since the T. aestivum comprises an allohexaploid genome, TaCaCA genes were derived from each A, B and D subgenome and homeologous chromosome (HC, except chromosome-group 1. Majority of genes were derived from more than one HCs in each family that were considered as homeologous genes (HGs due to their high similarity with each other. These HGs showed comparable gene and protein structures in terms of exon/intron organization and domain architecture. Majority of TaCaCA proteins comprised two Na_Ca_ex domains. However, TaNCLs consisted of an additional EF-hand domain with calcium binding motifs. Each TaCaCA protein family consisted of about ten transmembrane and two α-repeat regions with specifically conserved signature motifs except TaNCL, which had single α-repeat. Variable expression of most of the TaCaCA genes during various developmental stages suggested their specified role in development. However, constitutively high expression of a few genes like TaCAX1-A and TaNCL1-B indicated their role throughout the plant growth and development. The modulated expression of certain genes during biotic (fungal infections and abiotic stresses (heat, drought, salt suggested their role in stress response. Majority of TaCCX and TaNCL family genes were found highly affected during various abiotic stresses. However the role of individual gene needs to be established. The present study unfolded the opportunity

  20. Ca2+/Cation Antiporters (CaCA): Identification, Characterization and Expression Profiling in Bread Wheat (Triticum aestivum L.).

    Science.gov (United States)

    Taneja, Mehak; Tyagi, Shivi; Sharma, Shailesh; Upadhyay, Santosh Kumar

    2016-01-01

    The Ca 2+ /cation antiporters (CaCA) superfamily proteins play vital function in Ca 2+ ion homeostasis, which is an important event during development and defense response. Molecular characterization of these proteins has been performed in certain plants, but they are still not characterized in Triticum aestivum (bread wheat). Herein, we identified 34 TaCaCA superfamily proteins, which were classified into TaCAX, TaCCX, TaNCL, and TaMHX protein families based on their structural organization and evolutionary relation with earlier reported proteins. Since the T. aestivum comprises an allohexaploid genome, TaCaCA genes were derived from each A, B, and D subgenome and homeologous chromosome (HC), except chromosome-group 1. Majority of genes were derived from more than one HCs in each family that were considered as homeologous genes (HGs) due to their high similarity with each other. These HGs showed comparable gene and protein structures in terms of exon/intron organization and domain architecture. Majority of TaCaCA proteins comprised two Na_Ca_ex domains. However, TaNCLs consisted of an additional EF-hand domain with calcium binding motifs. Each TaCaCA protein family consisted of about 10 transmembrane and two α-repeat regions with specifically conserved signature motifs except TaNCL, which had single α-repeat. Variable expression of most of the TaCaCA genes during various developmental stages suggested their specified role in development. However, constitutively high expression of a few genes like TaCAX1-A and TaNCL1-B indicated their role throughout the plant growth and development. The modulated expression of certain genes during biotic (fungal infections) and abiotic stresses (heat, drought, salt) suggested their role in stress response. Majority of TaCCX and TaNCL family genes were found highly affected during various abiotic stresses. However, the role of individual gene needs to be established. The present study unfolded the opportunity for detail

  1. Weak transition of 44Ca

    International Nuclear Information System (INIS)

    Tauhata, L.

    1971-01-01

    A study of the nuclear core contribution to the excited nuclear states was done, along with its theoretical evaluation and interpretation, in the light nuclei region. The 727 KeV gamma ray transition between the 1885 KeV O + and 1157 KeV - 2 + states was detected, putting in evidence experimentally, the possibility of the 0 + state, characterized as core excitation, and found to be by + decay or electron capture from 44,44m Sc. The formation of the 2850 KeV state, due to 880 KeV transition from the 3667 KeV state, is re-discussed. The 564 KeV and 1684 KeV transitions from the 2850 KeV state to the 2285 KeV and 1157 KeV states, respectively, are also discussed. A new scheme is proposed for the 44 Ca excited states. (Author) [pt

  2. Effects of carbonated mineral water treatment in Băile Tuşnad on chronic arterial occlusive disease – a case report

    Directory of Open Access Journals (Sweden)

    Gabriela Dogaru

    2017-05-01

    Full Text Available Introduction. Băile Tușnad spa is recognized for its role in the prevention and rehabilitation treatment of cardiovascular diseases, including chronic arterial occlusive disease, due to the presence of natural therapeutic factors: carbonated mineral waters through their peripheral vasodilator effects, natural mofettes, stimulating bioclimate. Aim. The current study aimed to assess the clinical efficiency of natural therapeutic factors in Băile Tuşnad for the continuation of rehabilitation treatment in a patient with chronic arterial occlusive disease, in order to encourage walking, reduce cardiovascular risk and improve quality of life. Material and method. Patient N.M., aged 75, with multiple cardiovascular risk factors. In 2013, he was diagnosed with lower limb peripheral ischemia syndrome stage II B Fontaine, predominantly left claudication at about 100 m, for which balloon angioplasty was performed. He attended rehabilitation treatment for 3 years in Baile Tuşnad, consisting of carbonated mineral water baths for 15 minutes, aerotherapy for 30 minutes daily for the stimulation of walking, massotherapy, kinesiotherapy, performed daily for 16 days, and in 2016, at the Rehabilitation Hospital Cluj-Napoca. He was clinically evaluated before and at the end of treatment by the Visual Analogue Scale, the 10-m walking test, adverse reactions, Doppler ultrasound. Results. At the end of treatment, an increase in the walking distance and speed, a significant improvement in the quality of gait was found; claudication occurred after 250 m, pain in the lower limbs was improved. There were no side reactions. Conclusions. Rehabilitation treatment with natural therapeutic factors influenced the clinical and functional picture, determining a significant improvement in the quality of gait and quality of life.

  3. TU-E-BRA-05: Reverse Geometry Imaging with MV Detector for Improved Image Resolution.

    Science.gov (United States)

    Ganguly, A; Abel, E; Sun, M; Fahrig, R; Virshup, G; Star-Lack, J

    2012-06-01

    by NIH (grant number NIH R01 CA138426). © 2012 American Association of Physicists in Medicine.

  4. TU-AB-BRA-01: Abdominal Synthetic CT Generation in Support of Liver SBRT Dose Calculation

    Energy Technology Data Exchange (ETDEWEB)

    Bredfeldt, JS; Liu, L; Feng, M [University of Michigan, Ann Arbor, MI (United States); Cao, Y [The University of Michigan, Ann Arbor, MI (United States); Balter, J [University Michigan, Ann Arbor, MI (United States)

    2016-06-15

    Purpose: To demonstrate and validate a technique for generating MRI-derived synthetic CT volumes (MRCTs) in support of adaptive liver SBRT. Methods: Under IRB approval, ten hepatocellular carcinoma patients were scanned using a single MR sequence (T1 Dixon-VIBE), yielding inherently-registered water, fat, and T1-weighted images. Air-containing voxels were identified by intensity thresholding. The envelope of the anterior vertebral bodies was segmented from the fat image by fitting a shape model to vertebral body candidate voxels, then using level sets to expand the contour outward. Fuzzy-C-Means (FCM) was then used to classify each non-air voxel in the image as fat, water, bone, or marrow. Bone and marrow only were classified within the vertebral body envelope. The MRCT was created by integrating the product of the FCM class probability with the assigned class density for each voxel. The resulting MRCTs were deformably aligned with planning CTs and 2-ARC SBRT VMAT plans were optimized on the MRCT density maps. Fluence was copied onto the CT density grids and dose recalculated. Results: The MRCTs faithfully reproduced most of the features visible in the corresponding CT image volumes, with exceptions of ribs and posterior spinous processes. The liver, vertebral bodies, kidneys, spleen and cord all had median HU differences of less than 75 between MRCT and CT images. PTV D99% values had an average 0.2% difference (standard deviation: 0.46%) between calculations on MRCT and CT density grids. The maximum difference in dose to 0.1cc of the PTV was 0.25% (std:0.49%). OAR dose differences were similarly small (mean:0.03Gy, std:0.26Gy). The largest normal tissue complication percentage (NTCP) difference was 1.48% (mean:0.06%, std:0.54%). Conclusions: MRCTs from a single abdominal imaging sequence are promising for use in SBRT dose calculation. Future work will focus on extending models to better define bones in the upper abdomen. Supported by NIHR01EB016079 and NIH1L30CA

  5. TU-G-BRB-04: Digital Phantoms for Developing Protocols in Particle Therapy

    International Nuclear Information System (INIS)

    Lee, C.

    2015-01-01

    development and support. Research reported in this presentation is supported by the National Cancer Institute of the National; Institutes of Health under Award Number P20CA183640

  6. TU-F-17A-03: An Analytical Respiratory Perturbation Model for Lung Motion Prediction

    Energy Technology Data Exchange (ETDEWEB)

    Li, G; Yuan, A [Memorial Sloan Kettering Cancer Center, New York, NY (United States); Wei, J [City College of New York, New York, NY (United States)

    2014-06-15

    training, and therefore is potentially more resilient to breathing irregularities. On-going investigation introduces airflow into the RMP model for improvement. This research is in part supported by NIH (U54CA137788/132378). AY would like to thank MSKCC summer medical student research program supported by National Cancer Institute and hosted by Department of Medical Physics at MSKCC.

  7. TU-H-206-03: Characterizing B1 Inhomogeneities in DCE MRI

    International Nuclear Information System (INIS)

    Gach, H; Mason, N

    2016-01-01

    Purpose: Dynamic Contrast Enhanced (DCE) MRI is a valuable technique for measuring perfusion and permeability characteristics of tumors. Exogenous contrast concentrations are calculated based on changes in T 1 measured using fast 3D gradient echo (FLASH) sequences. However, the slab selective pulses used in 3D MRI may result in B 1 inhomogeneities across the volume of interest that can lead to errors in T 1 and thus the estimated gadolinium concentration. We compared three FLASH DCE sequences (GRE, TWIST, and VIBE) to determine their signal homogeneity across slices and the accuracy in calculating T 1 using acquisitions with variable flip angles. Methods: The sequences were tested at 3 T on a Siemens mMR (VB20P) using a doped water phantom 3.75 g/L NiSO 4 - 6H 2 O + 5 g/L NaCl (T 1 = 104 ms) and a 2% agar, 0.67% NaCl phantom (T 1 = 1.71 s). 2D EPI B 1 maps and inversion recovery T 1 maps were acquired for ground truth. 3D MRI was acquired at different flip angles to generate a T 1 map. Regions of interest were drawn to measure signal inside the phantoms as a function of slice position. The T 1 for each slice ROI was fit to the FLASH steady-state model of magnetization. Results: Based on the data, GRE gave the most uniform signal homogeneity and T 1 values in the middle slices of the 3D volume. The 3D VIBE sequence had the largest region of signal inhomogeneity compared to the 3D GRE and TWIST sequences. VIBE’s B 1 inhomogeneity is inconsistent at low flip angles. However, VIBE resulted in more slices with T 1 values similar to the ground truth. Conclusion: The central 1/3 of the slices yielded signals that result in T 1 fits consistent with the ground truth. However, the remaining slices required some form of B 1 inhomogeneity correction for quantitative DCE analysis. The research was supported in part by NIH NCI Grant R01CA159471.

  8. TU-G-BRB-04: Digital Phantoms for Developing Protocols in Particle Therapy

    Energy Technology Data Exchange (ETDEWEB)

    Lee, C. [National Cancer Institute (United States)

    2015-06-15

    development and support. Research reported in this presentation is supported by the National Cancer Institute of the National; Institutes of Health under Award Number P20CA183640.

  9. TU-G-BRB-03: IROC Houston’s Proton Beam Validation for Clinical Trials

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, P. [UT MD Anderson Cancer Center (United States)

    2015-06-15

    development and support. Research reported in this presentation is supported by the National Cancer Institute of the National; Institutes of Health under Award Number P20CA183640.

  10. TU-G-BRB-02: Clinical Trials in Particle Therapy - Open Questions

    Energy Technology Data Exchange (ETDEWEB)

    Choy, H. [UT Southwestern Medical Center (United States)

    2015-06-15

    development and support. Research reported in this presentation is supported by the National Cancer Institute of the National; Institutes of Health under Award Number P20CA183640.

  11. TU-G-BRB-00: Clinical Trials in Proton and Particle Therapy

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-06-15

    development and support. Research reported in this presentation is supported by the National Cancer Institute of the National; Institutes of Health under Award Number P20CA183640.

  12. TU-H-206-03: Characterizing B1 Inhomogeneities in DCE MRI

    Energy Technology Data Exchange (ETDEWEB)

    Gach, H [Washington University in St. Louis, St. Louis, MO (United States); Mason, N [University of Pittsburgh, Pittsburgh, PA (United States)

    2016-06-15

    Purpose: Dynamic Contrast Enhanced (DCE) MRI is a valuable technique for measuring perfusion and permeability characteristics of tumors. Exogenous contrast concentrations are calculated based on changes in T{sub 1} measured using fast 3D gradient echo (FLASH) sequences. However, the slab selective pulses used in 3D MRI may result in B{sub 1} inhomogeneities across the volume of interest that can lead to errors in T{sub 1} and thus the estimated gadolinium concentration. We compared three FLASH DCE sequences (GRE, TWIST, and VIBE) to determine their signal homogeneity across slices and the accuracy in calculating T{sub 1} using acquisitions with variable flip angles. Methods: The sequences were tested at 3 T on a Siemens mMR (VB20P) using a doped water phantom 3.75 g/L NiSO{sub 4} - 6H{sub 2}O + 5 g/L NaCl (T{sub 1} = 104 ms) and a 2% agar, 0.67% NaCl phantom (T{sub 1}= 1.71 s). 2D EPI B{sub 1} maps and inversion recovery T{sub 1}maps were acquired for ground truth. 3D MRI was acquired at different flip angles to generate a T{sub 1} map. Regions of interest were drawn to measure signal inside the phantoms as a function of slice position. The T{sub 1} for each slice ROI was fit to the FLASH steady-state model of magnetization. Results: Based on the data, GRE gave the most uniform signal homogeneity and T{sub 1} values in the middle slices of the 3D volume. The 3D VIBE sequence had the largest region of signal inhomogeneity compared to the 3D GRE and TWIST sequences. VIBE’s B{sub 1} inhomogeneity is inconsistent at low flip angles. However, VIBE resulted in more slices with T{sub 1} values similar to the ground truth. Conclusion: The central 1/3 of the slices yielded signals that result in T{sub 1} fits consistent with the ground truth. However, the remaining slices required some form of B{sub 1} inhomogeneity correction for quantitative DCE analysis. The research was supported in part by NIH NCI Grant R01CA159471.

  13. Investigation of functional aspects of the N-terminal region of elongation factor Tu from Escherichia coli using a protein engineering approach

    DEFF Research Database (Denmark)

    Laurberg, M; Mansilla, Francisco; Clark, Brian F. C.

    1998-01-01

    The function of the N-terminal region of elongation factor Tu is still unexplained. Until recently, it has not been visible in electron density maps from x-ray crystallography studies, but the presence of several well conserved basic residues suggest that this part of the molecule is of structural...... importance for the factor to function properly. In this study, two lysines at positions 4 and 9 were mutated separately to alanine or glutamate. The resulting four point mutants were expressed and purified using the pGEX system. The untagged products were characterized with regard to guanine...

  14. Optimal power flow based TU/CHP/PV/WPP coordination in view of wind speed, solar irradiance and load correlations

    International Nuclear Information System (INIS)

    Azizipanah-Abarghooee, Rasoul; Niknam, Taher; Malekpour, Mostafa; Bavafa, Farhad; Kaji, Mahdi

    2015-01-01

    Highlights: • Formulate probabilistic OPF with VPE, multi-fuel options, POZs, FOR of CHP units. • Propose a new powerful optimization method based on enhanced black hole algorithm. • Coordinate of TUs, WPPs, PVs and CHP units together in the proposed problem. • Evaluate the impacts of inputs’ uncertainties and their correlations on the POPF. • Use the 2m + 1 point estimated method. - Abstract: This paper addresses a novel probabilistic optimisation framework for handling power system uncertainties in the optimal power flow (OPF) problem that considers all the essential factors of great impact in the OPF problem. The object is to study and model the correlation and fluctuation of load demands, photovoltaic (PV) and wind power plants (WPPs) which have an important influence on transmission lines and bus voltages. Moreover, as an important tool of saving waste heat energy in the thermoelectric power plant, the power networks share of combined heat and power (CHP) has increased dramatically in the past decade. So, the probabilistic OPF (POPF) problem considering valve point effects, multi-fuel options and prohibited zones of thermal units (TUs) is firstly formulated. The PV, WPP and CHP units are also modeled. Then, a new method utilizing enhanced binary black hole (EBBH) algorithm and 2m + 1 point estimated method is proposed to solve this problem and to handle the random nature of solar irradiance, wind speed and load of consumers. The correlation between input random variables is considered using a correlation matrix. Finally, numerical results are presented and considered regarding the IEEE 118-busses, including PV, WPP, CHP and TU at several busses. The simulation and comparison results obtained demonstrate the broad advantages and feasibility of the suggested framework in the presence of dependent non-Gaussian distribution of random variables

  15. Role of Ca2+, membrane excitability, and Ca2+ stores in failing muscle contraction with aging.

    Science.gov (United States)

    Payne, Anthony Michael; Jimenez-Moreno, Ramón; Wang, Zhong-Ming; Messi, María Laura; Delbono, Osvaldo

    2009-04-01

    Excitation-contraction (EC) coupling in a population of skeletal muscle fibers of aged mice becomes dependent on the presence of external Ca(2+) ions (Payne, A.M., Zheng, Z., Gonzalez, E., Wang, Z.M., Messi, M.L., Delbono, O., 2004b. External Ca(2+)-dependent excitation - contraction coupling in a population of aging mouse skeletal muscle fibers. J. Physiol. 560, 137-155.). However, the mechanism(s) underlying this process remain unknown. In this work, we examined the role of (1) extracellular Ca(2+); (2) voltage-induced influx of external Ca(2+) ions; (3) sarcoplasmic reticulum (SR) Ca(2+) depletion during repeated contractions; (4) store-operated Ca(2+) entry (SOCE); (5) SR ultrastructure; (6) SR subdomain localization of the ryanodine receptor; and (7) sarcolemmal excitability in muscle force decline with aging. These experiments show that external Ca(2+), but not Ca(2+) influx, is needed to maintain force upon repetitive fiber electrical stimulation. Decline in fiber force is associated with depressed SR Ca(2+) release. SR Ca(2+) depletion, SOCE, and the putative segregated Ca(2+) release store do not play a significant role in external Ca(2+)-dependent contraction. More importantly, a significant number of action potentials fail in senescent mouse muscle fibers subjected to a stimulation frequency. These results indicate that failure to generate action potentials accounts for decreased intracellular Ca(2+) mobilization and tetanic force in aging muscle exposed to a Ca(2+)-free medium.

  16. Ca2+ cycling in heart cells from ground squirrels: adaptive strategies for intracellular Ca2+ homeostasis.

    Directory of Open Access Journals (Sweden)

    Xiao-Chen Li

    Full Text Available Heart tissues from hibernating mammals, such as ground squirrels, are able to endure hypothermia, hypoxia and other extreme insulting factors that are fatal for human and nonhibernating mammals. This study was designed to understand adaptive mechanisms involved in intracellular Ca(2+ homeostasis in cardiomyocytes from the mammalian hibernator, ground squirrel, compared to rat. Electrophysiological and confocal imaging experiments showed that the voltage-dependence of L-type Ca(2+ current (I(Ca was shifted to higher potentials in ventricular myocytes from ground squirrels vs. rats. The elevated threshold of I(Ca did not compromise the Ca(2+-induced Ca(2+ release, because a higher depolarization rate and a longer duration of action potential compensated the voltage shift of I(Ca. Both the caffeine-sensitive and caffeine-resistant components of cytosolic Ca(2+ removal were more rapid in ground squirrels. Ca(2+ sparks in ground squirrels exhibited larger amplitude/size and much lower frequency than in rats. Due to the high I(Ca threshold, low SR Ca(2+ leak and rapid cytosolic Ca(2+ clearance, heart cells from ground squirrels exhibited better capability in maintaining intracellular Ca(2+ homeostasis than those from rats and other nonhibernating mammals. These findings not only reveal adaptive mechanisms of hibernation, but also provide novel strategies against Ca(2+ overload-related heart diseases.

  17. Ground-penetrating radar investigation of St. Leonard's Crypt under the Wawel Cathedral (Cracow, Poland) - COST Action TU1208

    Science.gov (United States)

    Benedetto, Andrea; Pajewski, Lara; Dimitriadis, Klisthenis; Avlonitou, Pepi; Konstantakis, Yannis; Musiela, Małgorzata; Mitka, Bartosz; Lambot, Sébastien; Żakowska, Lidia

    2016-04-01

    The Wawel ensemble, including the Royal Castle, the Wawel Cathedral and other monuments, is perched on top of the Wawel hill immediately south of the Cracow Old Town, and is by far the most important collection of buildings in Poland. St. Leonard's Crypt is located under the Wawel Cathedral of St Stanislaus BM and St Wenceslaus M. It was built in the years 1090-1117 and was the western crypt of the pre-existing Romanesque Wawel Cathedral, so-called Hermanowska. Pope John Paul II said his first Mass on the altar of St. Leonard's Crypt on November 2, 1946, one day after his priestly ordination. The interior of the crypt is divided by eight columns into three naves with vaulted ceiling and ended with one apse. The tomb of Bishop Maurus, who died in 1118, is in the middle of the crypt under the floor; an inscription "+ MAVRVS EPC MCXVIII +" indicates the burial place and was made in 1938 after the completion of archaeological works which resulted in the discovery of this tomb. Moreover, the crypt hosts the tombs of six Polish kings and heroes: Michał Korybut Wiśniowiecki (King of the Polish-Lithuanian Commonwealth), Jan III Sobieski (King of the Polish-Lithuanian Commonwealth and Commander at the Battle of Vienna), Maria Kazimiera (Queen of the Polish-Lithuanian Commonwealth and consort to Jan III Sobieski), Józef Poniatowski (Prince of Poland and Marshal of France), Tadeusz Kościuszko (Polish general, revolutionary and a Brigadier General in the American Revolutionary War) and Władysław Sikorski (Prime Minister of the Polish Government in Exile and Commander-in-Chief of the Polish Armed Forces). The adjacent six crypts and corridors host the tombs of the other Polish kings, from Sigismund the Old to Augustus II the Strong, their families and several Polish heroes. In May 2015, the COST (European COoperation in Science and Technology) Action TU1208 "Civil engineering applications of Ground Penetrating Radar" organised and offered a Training School (TS) on the

  18. Exchanging knowledge and working together in COST Action TU1208: Short-Term Scientific Missions on Ground Penetrating Radar

    Science.gov (United States)

    Santos Assuncao, Sonia; De Smedt, Philippe; Giannakis, Iraklis; Matera, Loredana; Pinel, Nicolas; Dimitriadis, Klisthenis; Giannopoulos, Antonios; Sala, Jacopo; Lambot, Sébastien; Trinks, Immo; Marciniak, Marian; Pajewski, Lara

    2015-04-01

    This work aims at presenting the scientific results stemming from six Short-Term Scientific Missions (STSMs) funded by the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar' (Action Chair: Lara Pajewski, STSM Manager: Marian Marciniak). STSMs are important means to develop linkages and scientific collaborations between participating institutions involved in a COST Action. Scientists have the possibility to go to an institution abroad, in order to undertake joint research and share techniques/equipment/infrastructures that may not be available in their own institution. STSMs are particularly intended for Early Stage Researchers (ESRs), i.e., young scientists who obtained their PhD since no more than 8 years when they started to be involved in the Action. Duration of a standard STSM can be from 5 to 90 days and the research activities carried out during this short stay shall specifically contribute to the achievement of the scientific objectives of the supporting COST Action. The first STSM was carried out by Lara Pajewski, visiting Antonis Giannopoulos at The University of Edinburgh (United Kingdom). The research activities focused on the electromagnetic modelling of Ground Penetrating Radar (GPR) responses to complex targets. A set of test scenarios was defined, to be used by research groups participating to Working Group 3 of COST Action TU1208, to test and compare different electromagnetic forward- and inverse-scattering methods; these scenarios were modelled by using the well-known finite-difference time-domain simulator GprMax. New Matlab procedures for the processing and visualization of GprMax output data were developed. During the second STSM, Iraklis Giannakis visited Lara Pajewski at Roma Tre University (Italy). The study was concerned with the numerical modelling of horn antennas for GPR. An air-coupled horn antenna was implemented in GprMax and tested in a realistically

  19. Lattice Dynamics of fcc Ca

    DEFF Research Database (Denmark)

    Stassis, C.; Zaretsky, J.; Misemer, D. K.;

    1983-01-01

    A large single crystal of FCC Ca was grown and was used to study the lattice dynamics of this divalent metal by coherent inelastic neutron scattering. The phonon dispersion curves were measured, at room temperature, along the [ξ00], [ξξ0], [ξξξ], and [0ξ1] symmetry directions. The dispersion curves...... to the propagation of elastic waves. The frequencies of the T1[ξξ0] branch for ξ between approximately 0.5 and 0.8 are slightly above the velocity-of-sound line determined from the low-frequency measurements. Since a similar effect has been observed in FCC Yb, it is natural to assume that the anomalous dispersion.......8, there is a relative decrease in the electronic screening of the vibrational motion of the nuclei, which may account for the positive dispersion exhibited by the T1 [ξξ0] branch in this range of ξ values. The data were used to evaluate the elastic constants, the phonon density of states, and the lattice specific heat...

  20. ELEKTROMAGNĒTISKI IETEKMĒTU HIDRODINAMISKO PROCESU EKSPERIMENTĀLI PĒTĪJUMI ŠĶIDRU METĀLU NOSLĒGTOS TILPUMOS

    OpenAIRE

    Andris Bojarevičs

    2014-01-01

    ANOTĀCIJA Promocijas darbs veidots kā paplašināts kopsavilkums par 18 zinātnisku publikāciju kopu, kas veltīta šķidru metālu kustības dinamikai noslēgtos tilpumos apstākļos, kad to ietekmē ārēji uzlikts magnētiskais lauks, kā pastāvīgs, tā arī laikā mainīgs. Praktiski visi darbi ir autora plānotu un realizētu fizikālu eksperimentālu modeļu izpētes izklāsts, kas iekļaujas magnētiskās hidrodinamikas jomā. Darbā atspoguļoti eksperimentālie rezultāti par dažādu ārēji rosinātu faktoru veidotu ...

  1. Raúl Gonzalez Tuñón desencuadernado. Políticas de la literatura, entre el libro y las publicaciones periódicas

    Directory of Open Access Journals (Sweden)

    Geraldine Rogers

    2015-11-01

    Full Text Available RESUMEN: el artículo revisa algunos supuestos sobre la producción literaria de Raúl González Tuñón en las décadas de 1920 y 1930 a partir de dos desplazamientos. En primer lugar, explora las políticas de la literatura que permitirían pensar, de manera menos esquemática que con la habitual categoría de “literatura política” con que se suelen abordar sus textos de la década del treinta, las apuestas y limitaciones de una escritura que desde su inicio se propuso como transformadora. En segundo lugar, el artículo pone el foco en la primera circulación de los textos, muchas veces en diarios y revistas, contextos formativos de una literatura que en gran medida surgió en sus páginas. Desencuadernar a Tuñón implica atender a la materialidad de sus escritos y explorar más allá de la tradicional figura del autor de libros, para indagar políticas de la escritura que articularon campos y prácticas, y experimentaron no sólo con nuevas formas estéticas sino también con diversos modos de circulación pública de las letras.

  2. Coupled Ca2+/H+ transport by cytoplasmic buffers regulates local Ca2+ and H+ ion signaling.

    Science.gov (United States)

    Swietach, Pawel; Youm, Jae-Boum; Saegusa, Noriko; Leem, Chae-Hun; Spitzer, Kenneth W; Vaughan-Jones, Richard D

    2013-05-28

    Ca(2+) signaling regulates cell function. This is subject to modulation by H(+) ions that are universal end-products of metabolism. Due to slow diffusion and common buffers, changes in cytoplasmic [Ca(2+)] ([Ca(2+)]i) or [H(+)] ([H(+)]i) can become compartmentalized, leading potentially to complex spatial Ca(2+)/H(+) coupling. This was studied by fluorescence imaging of cardiac myocytes. An increase in [H(+)]i, produced by superfusion of acetate (salt of membrane-permeant weak acid), evoked a [Ca(2+)]i rise, independent of sarcolemmal Ca(2+) influx or release from mitochondria, sarcoplasmic reticulum, or acidic stores. Photolytic H(+) uncaging from 2-nitrobenzaldehyde also raised [Ca(2+)]i, and the yield was reduced following inhibition of glycolysis or mitochondrial respiration. H(+) uncaging into buffer mixtures in vitro demonstrated that Ca(2+) unloading from proteins, histidyl dipeptides (HDPs; e.g., carnosine), and ATP can underlie the H(+)-evoked [Ca(2+)]i rise. Raising [H(+)]i tonically at one end of a myocyte evoked a local [Ca(2+)]i rise in the acidic microdomain, which did not dissipate. The result is consistent with uphill Ca(2+) transport into the acidic zone via Ca(2+)/H(+) exchange on diffusible HDPs and ATP molecules, energized by the [H(+)]i gradient. Ca(2+) recruitment to a localized acid microdomain was greatly reduced during intracellular Mg(2+) overload or by ATP depletion, maneuvers that reduce the Ca(2+)-carrying capacity of HDPs. Cytoplasmic HDPs and ATP underlie spatial Ca(2+)/H(+) coupling in the cardiac myocyte by providing ion exchange and transport on common buffer sites. Given the abundance of cellular HDPs and ATP, spatial Ca(2+)/H(+) coupling is likely to be of general importance in cell signaling.

  3. An Aqueous Ca-Ion Battery.

    Science.gov (United States)

    Gheytani, Saman; Liang, Yanliang; Wu, Feilong; Jing, Yan; Dong, Hui; Rao, Karun K; Chi, Xiaowei; Fang, Fang; Yao, Yan

    2017-12-01

    Multivalent-ion batteries are emerging as low-cost, high energy density, and safe alternatives to Li-ion batteries but are challenged by slow cation diffusion in electrode materials due to the high polarization strength of Mg- and Al-ions. In contrast, Ca-ion has a low polarization strength similar to that of Li-ion, therefore a Ca-ion battery will share the advantages while avoiding the kinetics issues related to multivalent batteries. However, there is no battery known that utilizes the Ca-ion chemistry due to the limited success in Ca-ion storage materials. Here, a safe and low-cost aqueous Ca-ion battery based on a highly reversible polyimide anode and a high-potential open framework copper hexacyanoferrate cathode is demonstrated. The prototype cell shows a stable capacity and high efficiency at both high and low current rates, with an 88% capacity retention and an average 99% coloumbic efficiency after cycling at 10C for 1000 cycles. The Ca-ion storage mechanism for both electrodes as well as the origin of the fast kinetics have been investigated. Additional comparison with a Mg-ion cell with identical electrodes reveals clear kinetics advantages for the Ca-ion system, which is explained by the smaller ionic radii and more facile desolvation of hydrated Ca-ions.

  4. Cuento: Sangre de caña

    Directory of Open Access Journals (Sweden)

    Segundo Benjamín Corredor

    1967-09-01

    Full Text Available El viento se convertía en murmullo contra las hojas de los cañaverales y contra los techos semipelados del rancho. También menguaba el calor que maduraba los plantíos de caña y que hacía más efusiva la sangre de los moradores.

  5. TU-E-201-02: Eye Lens Dosimetry From CT Perfusion Studies

    International Nuclear Information System (INIS)

    Zhang, D.

    2015-01-01

    awareness can lead to avoidance or even prevention. Learning Objectives: To understand recent changes in eye lens dose limits and thresholds for tissue reactions To understand different approaches to dose estimation for eye lens To learn about challenges in eye lens opacities among staff in interventional fluoroscopy Di Zhang, Toshiba America Medical Systems, Tustin, CA, USA Eye lens radiation dose from brain perfusion CT exams CT perfusion imaging requires repeatedly exposing one location of the head to monitor the uptake and washout of iodinated contrast. The accumulated radiation dose to the eye lens can be high, leading to concerns about potential radiation injury from these scans. CTDIvol assumes continuous z coverage and can overestimate eye lens dose in CT perfusion scans where the table do not increment. The radiation dose to the eye lens from clinical CT brain perfusion studies can be estimated using Monte Carlo simulation methods on voxelized patient models. MDCT scanners from four major manufacturers were simulated and the eye lens doses were estimated using the AAPM posted clinical protocols. They were also compared to CTDIvol values to evaluate the overestimation from CTDIvol. The efficacy of eye lens dose reduction techniques such as tilting the gantry and moving the scan location away from the eyelens were also investigated. Eye lens dose ranged from 81 mGy to 279 mGy, depending on the scanner and protocol used. It is between 59% and 63% of the CTDIvol values reported by the scanners. The eye lens dose is significantly reduced when the eye lenses were not directly irradiated. CTDIvol should not be interpreted as patient dose; this study has shown it to overestimate dose to the eye lens. These results may be used to provide more accurate estimates of actual dose to ensure that protocols are operated safely below thresholds. Tilting the gantry or moving the scanning region further away from the eyes are effective for reducing lens dose in clinical practice

  6. TU-E-201-02: Eye Lens Dosimetry From CT Perfusion Studies

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, D. [Toshiba America Medical Systems (United States)

    2015-06-15

    awareness can lead to avoidance or even prevention. Learning Objectives: To understand recent changes in eye lens dose limits and thresholds for tissue reactions To understand different approaches to dose estimation for eye lens To learn about challenges in eye lens opacities among staff in interventional fluoroscopy Di Zhang, Toshiba America Medical Systems, Tustin, CA, USA Eye lens radiation dose from brain perfusion CT exams CT perfusion imaging requires repeatedly exposing one location of the head to monitor the uptake and washout of iodinated contrast. The accumulated radiation dose to the eye lens can be high, leading to concerns about potential radiation injury from these scans. CTDIvol assumes continuous z coverage and can overestimate eye lens dose in CT perfusion scans where the table do not increment. The radiation dose to the eye lens from clinical CT brain perfusion studies can be estimated using Monte Carlo simulation methods on voxelized patient models. MDCT scanners from four major manufacturers were simulated and the eye lens doses were estimated using the AAPM posted clinical protocols. They were also compared to CTDIvol values to evaluate the overestimation from CTDIvol. The efficacy of eye lens dose reduction techniques such as tilting the gantry and moving the scan location away from the eyelens were also investigated. Eye lens dose ranged from 81 mGy to 279 mGy, depending on the scanner and protocol used. It is between 59% and 63% of the CTDIvol values reported by the scanners. The eye lens dose is significantly reduced when the eye lenses were not directly irradiated. CTDIvol should not be interpreted as patient dose; this study has shown it to overestimate dose to the eye lens. These results may be used to provide more accurate estimates of actual dose to ensure that protocols are operated safely below thresholds. Tilting the gantry or moving the scanning region further away from the eyes are effective for reducing lens dose in clinical practice

  7. TU-E-201-00: Eye Lens Dosimetry for Patients and Staff

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-06-15

    awareness can lead to avoidance or even prevention. Learning Objectives: To understand recent changes in eye lens dose limits and thresholds for tissue reactions To understand different approaches to dose estimation for eye lens To learn about challenges in eye lens opacities among staff in interventional fluoroscopy Di Zhang, Toshiba America Medical Systems, Tustin, CA, USA Eye lens radiation dose from brain perfusion CT exams CT perfusion imaging requires repeatedly exposing one location of the head to monitor the uptake and washout of iodinated contrast. The accumulated radiation dose to the eye lens can be high, leading to concerns about potential radiation injury from these scans. CTDIvol assumes continuous z coverage and can overestimate eye lens dose in CT perfusion scans where the table do not increment. The radiation dose to the eye lens from clinical CT brain perfusion studies can be estimated using Monte Carlo simulation methods on voxelized patient models. MDCT scanners from four major manufacturers were simulated and the eye lens doses were estimated using the AAPM posted clinical protocols. They were also compared to CTDIvol values to evaluate the overestimation from CTDIvol. The efficacy of eye lens dose reduction techniques such as tilting the gantry and moving the scan location away from the eyelens were also investigated. Eye lens dose ranged from 81 mGy to 279 mGy, depending on the scanner and protocol used. It is between 59% and 63% of the CTDIvol values reported by the scanners. The eye lens dose is significantly reduced when the eye lenses were not directly irradiated. CTDIvol should not be interpreted as patient dose; this study has shown it to overestimate dose to the eye lens. These results may be used to provide more accurate estimates of actual dose to ensure that protocols are operated safely below thresholds. Tilting the gantry or moving the scanning region further away from the eyes are effective for reducing lens dose in clinical practice

  8. Electromagnetic modelling, inversion and data-processing techniques for GPR: ongoing activities in Working Group 3 of COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Giannopoulos, Antonis; van der Kruk, Jan

    2015-04-01

    This work aims at presenting the ongoing research activities carried out in Working Group 3 (WG3) 'EM methods for near-field scattering problems by buried structures; data processing techniques' of the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar' (www.GPRadar.eu). The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. WG3 is structured in four Projects. Project 3.1 deals with 'Electromagnetic modelling for GPR applications.' Project 3.2 is concerned with 'Inversion and imaging techniques for GPR applications.' The topic of Project 3.3 is the 'Development of intrinsic models for describing near-field antenna effects, including antenna-medium coupling, for improved radar data processing using full-wave inversion.' Project 3.4 focuses on 'Advanced GPR data-processing algorithms.' Electromagnetic modeling tools that are being developed and improved include the Finite-Difference Time-Domain (FDTD) technique and the spectral domain Cylindrical-Wave Approach (CWA). One of the well-known freeware and versatile FDTD simulators is GprMax that enables an improved realistic representation of the soil/material hosting the sought structures and of the GPR antennas. Here, input/output tools are being developed to ease the definition of scenarios and the visualisation of numerical results. The CWA expresses the field scattered by subsurface two-dimensional targets with arbitrary cross-section as a sum of cylindrical waves. In this way, the interaction is taken into account of multiple scattered fields within the medium hosting the sought targets. Recently, the method has been extended to deal with through-the-wall scenarios. One of the

  9. Solid Phase Extraction of Bryophyte Lipids / Briofītu lipīdu cietfāzes ekstrakcijas frakcionēšana

    Directory of Open Access Journals (Sweden)

    Klavina Laura

    2015-11-01

    Full Text Available Pētījuma ietvaros izstrādāta vienkārša, ātra un viegli atkārtojama analītiska metode briofītu lipīdu frakcionēšanai. Lipīdu frakcijas frakcionēšanai izmantoja cietfāzes ekstrakciju ar silikagēlu un ar NH2 grupām modificētu silikagēlu, iegūtās vielu grupas kvantificēja kā to trimetilsilil atvasinājumus, izmantojot gāzes hromatogrāfiju-masas spektrometriju. Frakcionēšanu veica, izmantojot eluentu polaritātes gradienta principu (eluenti: heksāns, heksāns/hloroforms 5:1, hloroforms, kā pēdējo eluentu ar NH2 grupām modificēta silikagēla gadījumā izmantojot skābes (etiķskābes šķīdumu. Izpētes objekti bija divi Latvijā plaši izplatīti sūnaugi (Polytrichum commune, Dicranum polysetum, kuru sastāvā identificēja vairāk nekā 45 dažādus savienojumus, daudzi no kuriem ir ar augstu bioloģisko aktivitāti. Sūnaugu sastāvā identificēja sterolus, tokoferolus, taukskābes un citi savienojumus. Izmantotā frakcionēšanas shēma pavēra iespējas identificēt daudzus savienojumus, kuri kopējā ekstrakta hromatogrammā nebija uzrādīti, piemēram, alkānu rindas savienojumi, vairāki steroli. Līdz ar to sūnaugu lipīdu cietfāzes frakcionēšanas metode ļāva noteikt vielu klātbūtni, kuras atrodas mikrodaudzumos, bet kuras varēja ļaut izsekot brīofītu pārvērtībām bioģeoķīmiskos procesos. Ieteiktā frakcionēšanas metode ļāva arī identificēt vairākas vielu grupas, kuras varētu būt perspektīvas kā jaunas bioloģiski aktīvas vielas, kā arī palīdzēja labāk izprast briofītu metabolismu, piemēram starpsugu atšķirības un sastāva sezonālās mainības raksturu.

  10. By Regulating Mitochondrial Ca2+-Uptake UCP2 Modulates Intracellular Ca2+.

    Directory of Open Access Journals (Sweden)

    Lukas Jaroslaw Motloch

    Full Text Available The possible role of UCP2 in modulating mitochondrial Ca2+-uptake (mCa2+-uptake via the mitochondrial calcium uniporter (MCU is highly controversial.Thus, we analyzed mCa2+-uptake in isolated cardiac mitochondria, MCU single-channel activity in cardiac mitoplasts, dual Ca2+-transients from mitochondrial ((Ca2+m and intracellular compartment ((Ca2+c in the whole-cell configuration in cardiomyocytes of wild-type (WT and UCP2-/- mice.Isolated mitochondria showed a Ru360 sensitive mCa2+-uptake, which was significantly decreased in UCP2-/- (229.4±30.8 FU vs. 146.3±23.4 FU, P0.05 and transsarcolemmal Ca2+-influx was inhibited suggesting a possible compensatory mechanism. Additionally, we observed an inhibitory effect of ATP on mCa2+-uptake in WT mitoplasts and (Ca2+m of cardiomyocytes leading to an increase of (Ca2+c while no ATP dependent effect was observed in UCP2-/-.Our results indicate regulatory effects of UCP2 on mCa2+-uptake. Furthermore, we propose, that previously described inhibitory effects on MCU by ATP may be mediated via UCP2 resulting in changes of excitation contraction coupling.

  11. Impact of seawater [Ca2+] on the calcification and calcite Mg / Ca of Amphistegina lessonii

    NARCIS (Netherlands)

    Mewes, A.; Langer, G.; Thoms, S.; Nehrke, G.; Reichart, G. J.; de Nooijer, L. J.; Bijma, J.

    2015-01-01

    Mg / Ca ratios in foraminiferal tests are routinely used as paleotemperature proxies, but on long timescales, they also hold the potential to reconstruct past seawater Mg / Ca. The impact of both temperature and seawater Mg / Ca on Mg incorporation in Foraminifera has been quantified by a number of

  12. Ca-Dependent Folding of Human Calumenin

    Science.gov (United States)

    Mazzorana, Marco; Hussain, Rohanah; Sorensen, Thomas

    2016-01-01

    Human calumenin (hCALU) is a six EF-hand protein belonging to the CREC family. As other members of the family, it is localized in the secretory pathway and regulates the activity of SERCA2a and of the ryanodine receptor in the endoplasmic reticulum (ER). We have studied the effects of Ca2+ binding to the protein and found it to attain a more compact structure upon ion binding. Circular Dichroism (CD) measurements suggest a major rearrangement of the protein secondary structure, which reversibly switches from disordered at low Ca2+ concentrations to predominantly alpha-helical when Ca2+ is added. SAXS experiments confirm the transition from an unfolded to a compact structure, which matches the structural prediction of a trilobal fold. Overall our experiments suggest that calumenin is a Ca2+ sensor, which folds into a compact structure, capable of interacting with its molecular partners, when Ca2+ concentration within the ER reaches the millimolar range. PMID:26991433

  13. CaMKII in the Cardiovascular System: Sensing Redox States

    Science.gov (United States)

    Erickson, Jeffrey R.; He, B. Julie; Grumbach, Isabella M.; Anderson, Mark E

    2013-01-01

    The multifunctional Ca2+ and calmodulin-dependent protein kinase II (CaMKII) is now recognized to play a central role in pathological events in the cardiovascular system. CaMKII has diverse downstream targets that promote vascular disease, heart failure and arrhythmias, so improved understanding of CaMKII signaling has the potential to lead to new therapies for cardiovascular disease. CaMKII is a multimeric serine-threonine kinase that is initially activated by binding calcified calmodulin (Ca2+/CaM). Under conditions of sustained exposure to elevated Ca2+/CaM CaMKII transitions into a Ca2+/CaM-autonomous enzyme by two distinct but parallel processes. Autophosphorylation of threonine 287 in the CaMKII regulatory domain ‘traps’ CaMKII into an open configuration even after Ca2+/CaM unbinding. More recently, our group identified a pair of methionines (281/282) in the CaMKII regulatory domain that undergo a partially reversible oxidation which, like autophosphorylation, prevents CaMKII from inactivating after Ca2+/CaM unbinding. Here we review roles of CaMKII in cardiovascular disease with an eye to understanding how CaMKII may act as a transduction signal to connect pro-oxidant conditions into specific downstream pathological effects that are relevant to rare and common forms of cardiovascular disease. PMID:21742790

  14. Za přečtení stojí... Kateřina Tučková: Vyhnání Gerty Schnirch. Brno, Host 2009

    Czech Academy of Sciences Publication Activity Database

    Košnarová, Veronika

    2010-01-01

    Roč. 60, č. 5 (2010), s. 237-240 ISSN 0009-0786 Institutional research plan: CEZ:AV0Z90560517 Keywords : Czech literature * Tučková, Kateřina * interpretations Subject RIV: AJ - Letters, Mass-media, Audiovision

  15. Sporadic Ca and Ca+ layers at mid-latitudes: Simultaneous observations and implications for their formation

    Directory of Open Access Journals (Sweden)

    M. Gerding

    2001-01-01

    Full Text Available We report on the observations of 188 sporadic layers of either Ca atoms and/or Ca ions that we have observed during 112 nights of lidar soundings of Ca, and 58 nights of Ca+ soundings, at Kühlungsborn, Germany (54° N, 12° E. The Ca+ soundings have been performed simultaneously and in a common volume with the Ca soundings by two separate lidars. Correlations between sporadic neutral and ionized metal layers are demonstrated through four case studies. A systematic study of the variations of occurrence of sporadic Ca and Ca+ layers reveals that neutral and ionized Ca layers are not as closely correlated as expected earlier: (a The altitude distribution shows the simultaneous occurrence of both sporadic Ca and Ca+ layers to be most likely only in the narrow altitude range between 90 and 95 km. Above that region, in the lower thermosphere, the sporadic ion layers are much more frequent than atom layers. Below 90 km only very few sporadic layers have been observed; (b The seasonal variation of sporadic Ca layers exhibits a minimum of occurrence in summer, while sporadic Ca+ layers do not show a significant seasonal variation (only the dense Ca+ layers appear to have a maximum in summer. At mid-latitudes sporadic Ca layers are more frequent than sporadic layers of other atmospheric metals like Na or K. For the explanation of our observations new formation mechanisms are discussed.Key words. Ionosphere (ion chemistry and composition; ionosphere-atmosphere interactions; mid-latitude ionosphere

  16. Results of measuring internal Ca absorption and Ca kinetics with /sup 47/Ca in patients suffering from chronically recurrent urolithiasis

    Energy Technology Data Exchange (ETDEWEB)

    Hoffmann, L.; Esther, G.; Serfling, D.; Bast, R. (Rostock Univ. (German Democratic Republic). Radiologische Klinik)

    1983-07-01

    47 patients with chronically recurrent calcium lithiasis (with between 3 and over 100 stone episodes) and an average age of 43.2 years have been examined. The Ca absorption lay between 42.7% and 90.0% of the dose, the average was 59.3% with a standard deviation of +- 12.9. This is higher than in persons with healthy kidneys. The Ca absorption is significantly lower in patients with renal insufficiency. Studies on Ca kinetics revealed turnover rates and pool sizes within the normal range.

  17. Results of measuring enteral Ca absorption and Ca kinetics with 47Ca in patients suffering from chronically recurrent urolithiasis

    International Nuclear Information System (INIS)

    Hoffmann, L.; Esther, G.; Serfling, D.; Bast, R.

    1983-01-01

    47 patients with chronically recurrent calcium lithiasis (with between 3 and over 100 stone episodes) and an average age of 43.2 years have been examined. The Ca absorption lay between 42.7% and 90.0% of the dose, the average was 59.3% with a standard deviation of +- 12.9. This is higher than in persons with healthy kidneys. The Ca absorption is significantly lower in patients with renal insufficiency. Studies on Ca kinetics revealed turnover rates and pool sizes within the normal range. (author)

  18. Ca2+-dependent structural rearrangements within Na+–Ca2+ exchanger dimers

    OpenAIRE

    John, Scott A.; Ribalet, Bernard; Weiss, James N.; Philipson, Kenneth D.; Ottolia, Michela

    2011-01-01

    Cytoplasmic Ca2+ is known to regulate Na+–Ca2+ exchanger (NCX) activity by binding to two adjacent Ca2+-binding domains (CBD1 and CBD2) located in the large intracellular loop between transmembrane segments 5 and 6. We investigated Ca2+-dependent movements as changes in FRET between exchanger proteins tagged with CFP or YFP at position 266 within the large cytoplasmic loop. Data indicate that the exchanger assembles as a dimer in the plasma membrane. Addition of Ca2+ decreases the distance be...

  19. Isospin effects in {sup 40,48}Ca+{sup 40,48}Ca collisions

    Energy Technology Data Exchange (ETDEWEB)

    Henzl, V., E-mail: henzl@mit.ed [MSU-NSCL, 1 Cyclotron, East Lansing MI, 48824 (United States); Henzlova, D.; Kilburn, M. [MSU-NSCL, 1 Cyclotron, East Lansing MI, 48824 (United States); Verde, G. [INFN, Sezione di Catania. 64 Via S. Sofia, I-95123, Catania (Italy); Brown, D. [MSU-NSCL, 1 Cyclotron, East Lansing MI, 48824 (United States); Chbihi, A. [GANIL, CEA et IN2P3-CNRS, B.P. 5027, F-14076 Caen Cedex (France); Coupland, D. [MSU-NSCL, 1 Cyclotron, East Lansing MI, 48824 (United States); Elson, J. [Departments of Chemistry and Physics, Washington University, St. Louis, MO 63130 (United States); Famiano, M. [Western Michigan University, 2229 Everett Tower, Kalamazoo, MI 49008-5252 (United States); Herlitzius, C. [MSU-NSCL, 1 Cyclotron, East Lansing MI, 48824 (United States); Hudan, S. [Indiana Univ. Cyclotron Facility, 2401 Milo B. Sampson Lane, Bloomington, IN 47405-4001 (United States); Lee, J.; Lukyanov, S.; Lynch, W.; Rogers, A.; Sanetullaev, A. [MSU-NSCL, 1 Cyclotron, East Lansing MI, 48824 (United States); Sobotka, L. [Departments of Chemistry and Physics, Washington University, St. Louis, MO 63130 (United States); Souza, R.T. de [Indiana Univ. Cyclotron Facility, 2401 Milo B. Sampson Lane, Bloomington, IN 47405-4001 (United States); Sun, Z.Y.; Tsang, B. [MSU-NSCL, 1 Cyclotron, East Lansing MI, 48824 (United States)

    2010-03-01

    The isospin dependence of two proton correlations is studied in {sup 40}Ca+{sup 40}Ca and {sup 48}Ca+{sup 48}Ca collisions at E/A=80MeV. Measurements were performed with the HiRA detector array complemented by the 4pi Ball at NSCL. We observe a strong isospin dependence of the pp-correlation functions; however the emitting source radius extracted using the imaging technique yields no sensitivity to the isospin of the reaction system. We interpret this result as a consequence of smaller fraction of fast proton emission in the neutron rich {sup 48}Ca system.

  20. Isospin effects in 40,48Ca+40,48Ca collisions

    International Nuclear Information System (INIS)

    Henzl, V.; Henzlova, D.; Kilburn, M.; Verde, G.; Brown, D.; Chbihi, A.; Coupland, D.; Elson, J.; Famiano, M.; Herlitzius, C.; Hudan, S.; Lee, J.; Lukyanov, S.; Lynch, W.; Rogers, A.; Sanetullaev, A.; Sobotka, L.; Souza, R.T. de; Sun, Z.Y.; Tsang, B.

    2010-01-01

    The isospin dependence of two proton correlations is studied in 40 Ca+ 40 Ca and 48 Ca+ 48 Ca collisions at E/A=80MeV. Measurements were performed with the HiRA detector array complemented by the 4π Ball at NSCL. We observe a strong isospin dependence of the pp-correlation functions; however the emitting source radius extracted using the imaging technique yields no sensitivity to the isospin of the reaction system. We interpret this result as a consequence of smaller fraction of fast proton emission in the neutron rich 48 Ca system.

  1. PGC-1α accelerates cytosolic Ca2+ clearance without disturbing Ca2+ homeostasis in cardiac myocytes

    International Nuclear Information System (INIS)

    Chen, Min; Wang, Yanru; Qu, Aijuan

    2010-01-01

    Energy metabolism and Ca 2+ handling serve critical roles in cardiac physiology and pathophysiology. Peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α) is a multi-functional coactivator that is involved in the regulation of cardiac mitochondrial functional capacity and cellular energy metabolism. However, the regulation of PGC-1α in cardiac Ca 2+ signaling has not been fully elucidated. To address this issue, we combined confocal line-scan imaging with off-line imaging processing to characterize calcium signaling in cultured adult rat ventricular myocytes expressing PGC-1α via adenoviral transduction. Our data shows that overexpressing PGC-1α improved myocyte contractility without increasing the amplitude of Ca 2+ transients, suggesting that myofilament sensitivity to Ca 2+ increased. Interestingly, the decay kinetics of global Ca 2+ transients and Ca 2+ waves accelerated in PGC-1α-expressing cells, but the decay rate of caffeine-elicited Ca 2+ transients showed no significant change. This suggests that sarcoplasmic reticulum (SR) Ca 2+ -ATPase (SERCA2a), but not Na + /Ca 2+ exchange (NCX) contribute to PGC-1α-induced cytosolic Ca 2+ clearance. Furthermore, PGC-1α induced the expression of SERCA2a in cultured cardiac myocytes. Importantly, overexpressing PGC-1α did not disturb cardiac Ca 2+ homeostasis, because SR Ca 2+ load and the propensity for Ca 2+ waves remained unchanged. These data suggest that PGC-1α can ameliorate cardiac Ca 2+ cycling and improve cardiac work output in response to physiological stress. Unraveling the PGC-1α-calcium handing pathway sheds new light on the role of PGC-1α in the therapy of cardiac diseases.

  2. Evaluation of Serum CEA, CA19-9, CA72-4, CA125 and Ferritin as Diagnostic Markers and Factors of Clinical Parameters for Colorectal Cancer.

    Science.gov (United States)

    Gao, Yanfeng; Wang, Jinping; Zhou, Yue; Sheng, Sen; Qian, Steven Y; Huo, Xiongwei

    2018-02-09

    Blood-based protein biomarkers have recently shown as simpler diagnostic modalities for colorectal cancer, while their association with clinical pathological characteristics is largely unknown. In this study, we not only examined the sensitivity and reliability of single/multiple serum markers for diagnosis, but also assessed their connection with pathological parameters from a total of 279 colorectal cancer patients. Our study shown that glycoprotein carcinoembryonic antigen (CEA) owns the highest sensitivity among single marker in the order of CEA > cancer antigen 72-4 (CA72-4) > cancer antigen 19-9 9 (CA19-9) > ferritin > cancer antigen 125 (CA125), while the most sensitive combined-markers for two to five were: CEA + CA72-4; CEA + CA72-4 + CA125; CEA + CA19-9 + CA72-4 + CA125; and CEA + CA19-9 + CA72-4 + CA125 + ferritin, respectively. We also demonstrated that patients who had positive preoperative serum CEA, CA19-9, or CA72-4 were more likely with lymph node invasion, positive CA125 were prone to have vascular invasion, and positive CEA or CA125 were correlated with perineural invasion. In addition, positive CA19-9, CA72-4, or CA125 was associated with poorly differentiated tumor, while CEA, CA19-9, CA72-4, CA125 levels were positively correlated with pathological tumor-node-metastasis stages. We here conclude that combined serum markers can be used to not only diagnose colorectal cancer, but also appraise the tumor status for guiding treatment, evaluation of curative effect, and prognosis of patients.

  3. Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles

    Directory of Open Access Journals (Sweden)

    Shiri M

    2016-11-01

    Full Text Available Mahdi Shiri,1,2,* Mona Navaei-Nigjeh,1,3,* Maryam Baeeri,1 Mahban Rahimifard,1 Hossein Mahboudi,4 Ahmad Reza Shahverdi,5 Abbas Kebriaeezadeh,1 Mohammad Abdollahi1,6,7 1Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 2School of Medicine, Artesh University of Medical Sciences, 3Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; 4Department of Biotechnology, Faculty of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 5Department of Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, 6Toxicology Interest Group, USERN, 7Endocrinology & Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran *These authors contributed equally to this work Abstract: Diazinon (DZ is an organophosphorus insecticide that acts as an acetylcholinesterase inhibitor. It is important to note that it can induce oxidative stress, lipid peroxidation, diabetic disorders, and cytotoxicity. Magnesium oxide (MgO and selenium nanoparticles (Se NPs showed promising protection against oxidative stress, lipid peroxidation, cytotoxicity, and diabetic disorders. Therefore, this study was conducted to explore the possible protective mechanisms of MgO and Se NPs against DZ-induced cytotoxicity in PaTu cell line. Cytotoxicity of DZ, in the presence or absence of effective doses of MgO and Se NPs, was determined in human pancreatic cancer cell line (PaTu cells after 24 hours of exposure by using mitochondrial activity and mitochondrial membrane potential assays. Then, the insulin, proinsulin, and C-peptide release; caspase-3 and -9 activities; and total thiol molecule levels were assessed. Determination of cell viability, including apoptotic and necrotic cells, was assessed via acridine orange/ethidium bromide double

  4. Regulated release of Ca2+ from respiring mitochondria by Ca2+/2H+ antiport.

    Science.gov (United States)

    Fiskum, G; Lehninger, A L

    1979-07-25

    Simultaneous measurements of oxygen consumption and transmembrane transport of Ca2+, H+, and phosphate show that the efflux of Ca2+ from respiring tightly coupled rat liver mitochondria takes place by an electroneutral Ca2+/2H+ antiport process that is ruthenium red-insensitive and that is regulated by the oxidation-reduction state of the mitochondrial pyridine nucleotides. When mitochondrial pyridine nucleotides are kept in a reduced steady state, the efflux of Ca2+ is inhibited; when they are in an oxidized state, Ca2+ efflux is activated. These processes were demonstrated by allowing phosphate-depleted mitochondria respiring on succinate in the presence of rotenone to take up Ca2+ from the medium. Upon subsequent addition of ruthenium red to block Ca2+ transport via the electrophoretic influx pathway, and acetoacetate, to bring mitochondrial pyridine nucleotides into the oxidized state, Ca2+ efflux and H+ influx ensued. The observed H+ influx/Ca2+ efflux ratio was close to the value 2.0 predicted for the operation of an electrically neutral Ca2+/2H+ antiport process.

  5. Reduced endogenous Ca2+ buffering speeds active zone Ca2+ signaling.

    Science.gov (United States)

    Delvendahl, Igor; Jablonski, Lukasz; Baade, Carolin; Matveev, Victor; Neher, Erwin; Hallermann, Stefan

    2015-06-09

    Fast synchronous neurotransmitter release at the presynaptic active zone is triggered by local Ca(2+) signals, which are confined in their spatiotemporal extent by endogenous Ca(2+) buffers. However, it remains elusive how rapid and reliable Ca(2+) signaling can be sustained during repetitive release. Here, we established quantitative two-photon Ca(2+) imaging in cerebellar mossy fiber boutons, which fire at exceptionally high rates. We show that endogenous fixed buffers have a surprisingly low Ca(2+)-binding ratio (∼ 15) and low affinity, whereas mobile buffers have high affinity. Experimentally constrained modeling revealed that the low endogenous buffering promotes fast clearance of Ca(2+) from the active zone during repetitive firing. Measuring Ca(2+) signals at different distances from active zones with ultra-high-resolution confirmed our model predictions. Our results lead to the concept that reduced Ca(2+) buffering enables fast active zone Ca(2+) signaling, suggesting that the strength of endogenous Ca(2+) buffering limits the rate of synchronous synaptic transmission.

  6. High CA-125 and CA19-9 levels in spontaneous ruptured ovarian endometriomas.

    Science.gov (United States)

    Dai, Xinyue; Jin, Chu; Hu, Yan; Zhang, Qian; Yan, Xiaojian; Zhu, Fangfang; Lin, Feng

    2015-10-23

    To evaluate the clinical significance of serum CA-125 and CA19-9 in women with spontaneous ruptured ovarian endometriomas. From January 2006 to April 2015, a total of 1653 women were diagnosed with ovarian endometriomas, and 43 women were diagnosed with the spontaneous rupture of their ovarian endometrioma. In addition, 70 women diagnosed with unruptured ovarian endometriomas were chosen to serve as control subjects. Serum CA-125 and CA19-9 levels, together with the clinical materials, were collected. Serum CA-125, CA19-9, and the combined biomarkers were shown to be obviously elevated in the spontaneous ruptured ovarian endometrioma group (p=0.001, p=0.001, p=0.001, respectively). The AUC value for the combined biomarkers was 0.992 (95% CI, 0.981-1.000), with a high sensitivity and specificity of nearly 100% and 93.6%, respectively. Moreover, the maximum diameter of the mass was significantly (p=0.001) increased in the ruptured group. Serum CA-125 and CA19-9 were significantly increased in patients with spontaneous ruptured ovarian endometriomas. Moreover, the combined biomarkers were better than either CA-125 or CA19-9 alone in the diagnosis of a spontaneous rupture of the ovarian endometrioma. Copyright © 2015 Elsevier B.V. All rights reserved.

  7. EnviroAtlas - Fresno, CA - Block Groups

    Data.gov (United States)

    U.S. Environmental Protection Agency — This EnviroAtlas dataset is the base layer for the Fresno, CA EnviroAtlas area. The block groups are from the US Census Bureau and are included/excluded based on...

  8. Role of Ca++ in Shoot Gravitropism. [avena

    Science.gov (United States)

    Rayle, D. L.

    1985-01-01

    A cornerstone in the argument that Ca(2+) levels may regulate growth is the finding the EGTA promotes straight growth. The usual explanation for these results is that Ca(2+) chelation from cell walls results in wall loosening and thus accelerated straight growth. The ability of frozen-thawed Avena coleoptile tissue (subjected to 15g tension) to extend in response to EGTA and Quin II was examined. The EGTA when applied in weakly buffered (i.e., 0.1mM) neutral solutions initiates rapid extension. When the buffer strength is increased, similar concentrations of EGTA produce no growth response. This implies when EGTA liberated protons are released upon Ca(2+) chelation they can either initiate acid growth (low buffer conditions) or if consumed (high buffer conditions) have no effect. Thus Ca(2+) chelation in itself apparently does not result in straight growth.

  9. YouthCaN 2001 / Sirje Janikson

    Index Scriptorium Estoniae

    Janikson, Sirje

    2001-01-01

    Aprillis 2001 toimus keskkonnateemaline õpilaskonverents YouthCaN 2001 Ameerika Loodusajaloo Muuseumis New Yorkìs. 35 seminarist ühe viis läbi Tartu Noorte Loodusmaja geoloogia ja keskkonnaringi esindus, tutvustati loodusmaja keskkonnaprojekte ja räägiti keskkonnaalaste veebilehtede koostamise kogemustest. YouthCaN (Youth Communicating and Networking) on rahvusvaheline noorte organisatsioon, mis vahendab kogemusi ja uusi ideid elukeskkonnast huvitatud noorte hulgas

  10. [Association of ovarian tumors with CA-125].

    Science.gov (United States)

    Martínez-Acosta, Judith Elena; Olguín-Cruces, Víctor Alberto

    2016-01-01

    The tumor marker CA-125 is the most widely used serum marker for ovarian cancer screening. The aim of this paper was to establish the association between histopathologic result of ovarian tumors with serologic CA-125 and utility for the diagnosis of ovarian tumors at a Ginecoobstetric hospital. An observational, retrospective, descriptive and longitudinal study, from September 1st 2010 to February 28 2013. All patients with histopathologic report ovarian tumor and CA-125 was selected to analyze the association of ovarian tumors with their histological type, biological behavior, range positivity of CA-125 and its relationship to the pre and postmenopausal state. Of 1213 patients, 334 were included. Utility of CA-125 in postmenopausal reported positive predictive value of 67.5%, with sensitivity (72%), specificity (82.6%) and negative predictive value (86.1%), both with p = 0.001, mainly in the epithelial origin. In premenopausal a low positive predictive value was reported. The CA-125 is useful for screening for ovarian cancer in postmenopausal women, mainly for epithelial origin.

  11. COST Action TU1206 "SUB-URBAN - A European network to improve understanding and use of the ground beneath our cities"

    Science.gov (United States)

    Campbell, Diarmad; de Beer, Johannes; Lawrence, David; van der Meulen, Michiel; Mielby, Susie; Hay, David; Scanlon, Ray; Campenhout, Ignace; Taugs, Renate; Eriksson, Ingelov

    2014-05-01

    Sustainable urbanisation is the focus of SUB-URBAN, a European Cooperation in Science and Technology (COST) Action TU1206 - A European network to improve understanding and use of the ground beneath our cities. This aims to transform relationships between experts who develop urban subsurface geoscience knowledge - principally national Geological Survey Organisations (GSOs), and those who can most benefit from it - urban decision makers, planners, practitioners and the wider research community. Under COST's Transport and Urban Development Domain, SUB-URBAN has established a network of GSOs and other researchers in over 20 countries, to draw together and evaluate collective urban geoscience research in 3D/4D characterisation, prediction and visualisation. Knowledge exchange between researchers and City-partners within 'SUB-URBAN' is already facilitating new city-scale subsurface projects, and is developing a tool-box of good-practice guidance, decision-support tools, and cost-effective methodologies that are appropriate to local needs and circumstances. These are intended to act as catalysts in the transformation of relationships between geoscientists and urban decision-makers more generally. As a result, the importance of the urban sub-surface in the sustainable development of our cities will be better appreciated, and the conflicting demands currently placed on it will be acknowledged, and resolved appropriately. Existing city-scale 3D/4D model exemplars are being developed by partners in the UK (Glasgow, London), Germany (Hamburg) and France (Paris). These draw on extensive ground investigation (10s-100s of thousands of boreholes) and other data. Model linkage enables prediction of groundwater, heat, SuDS, and engineering properties. Combined subsurface and above-ground (CityGML, BIMs) models are in preparation. These models will provide valuable tools for more holistic urban planning; identifying subsurface opportunities and saving costs by reducing uncertainty in

  12. Gigantes digitales. Quijote en 17000 tuits de Diego Buendía y Escribe tu propio Quijote de Belén Gache

    Directory of Open Access Journals (Sweden)

    Celia Corral Cañas

    2015-05-01

    Full Text Available To incorporate Don Quixote into the virtual environment offers many possibilities, from the literal adaptation into an electronic format, such as rewriting it on Twitter, to the allusion to the book in an avant-garde creation. Thus, among the various ways of tackling Don Quixote in Twitter, the project El Quijote en 17000 tuits by Diego Buendía, who makes a fragmented transcript in 17000 sections or tweets, stands out. Furthermore, Escribe tu propio Quijote by Belén Gache honors Cervantes’ novel with a simple interactive game that invites the reader to rethink the authorship issue within and outside the work. These two creations, born from the junction of the famous novel by Cervantes and the particularities of the new ecosystem, recover the quixotic nature to integrate it, from very different paths, into the digital universe.

  13. TuGen

    DEFF Research Database (Denmark)

    Gilling, Lasse

    An implementation of Mann's method of generating synthetic turbulence is described. A brief introduction to the method is given prior to some details on the implementation. Several tests to verify the implementation follows and finally the use of the program is described....

  14. Construye tu Música

    Directory of Open Access Journals (Sweden)

    Contreras, Antonio de

    2000-05-01

    Full Text Available En el Sistema Educativo actual la composición musical está contemplada en el tramo final de los estudios de Conservatorio, tras muchos años de adiestramiento técnico-instrumental y teórico. Por otra parte, si visitamos un Aula de Educación Plástica de un Instituto, podemos ver a los alumnos de Secundaria expresándose, canalizando su creatividad a través de colores, formas y volúmenes, sin prejuicios, con un mínimo y, en la mayoría de los casos, muy simple bagaje de conocimientos técnicos previos. Está claro que se trata de dos artes completamente diferentes, también es bien sabido y repetido hasta la saciedad el carácter abstracto de la música. Pero así y todo ¿Será posible arbitrar un modo de acercarse a la música desde dentro, desde la práctica de la composición musical de una forma directa y simple, sin necesidad de una formación musical de nivel superior, de forma parecida a como se plantea la actividad creativa en la educación plástica? ¿Será posible complementar dicha práctica con la subsiguiente interpretación de las composiciones y la crítica de las producciones ajenas del mismo nivel? Las preguntas no son, por supuesto, nuevas: ya los grandes pedagogos musicales del primer cuarto de siglo las plantearon y resolvieron cada uno a su modo. La siguiente comunicación pretende dar a conocer los resultados de una investigación en el Aula de música, realizada por el autor durante los cuatro últimos años, que parte de una propuesta original en este sentido.

  15. Preparation and characterization of Eu 3 activated CaSiO3,(CaA ...

    Indian Academy of Sciences (India)

    Eu3+ activated CaSiO3, (Ca, Ba) SiO3 and (Ca, Sr) SiO3 have been prepared by sol–gel technique. Residual solvent and organic contents in the gel were removed by firing at 100°C for 3–4 h at 300 and 600°C for 2 h. Small exothermic shoulder around 850 to 875°C, as observed in DTA curve, corresponds to crystallization ...

  16. Ca(2+-dependent regulation of the Ca(2+ concentration in the myometrium mitochondria. II. Ca(2+ effects on mitochondria membranes polarization and [Ca(2+](m

    Directory of Open Access Journals (Sweden)

    L. G. Babich

    2017-06-01

    Full Text Available It is known that Ca2+ accumulation in the mitochondria undergoes complex regulation by Ca2+ itself. But the mechanisms of such regulation are still discussed. In this paper we have shown that Ca ions directly or indirectly regulate the level of myometrium mitochondria membranes polarization. The additions of 100 µM Ca2+ were accompanied by depolarization of the mitochondria membranes. The following experiments were designed to study the impact of Ca2+ on the myometrium mitochondria [Ca2+]m. Isolated myometrium mitochondria were preincubated without or with 10 μM Са2+ followed by 100 μM Са2+ addition. Experiments were conducted in three mediums: without ATP and Mg2+ (0-medium, in the presence of 3 mM Mg2+ (Mg-medium and 3 mM Mg2+ + 3 mM ATP (Mg,ATP-medium. It was shown that the effects of 10 μM Са2+ addition were different in different mediums, namely in 0- and Mg-medium the [Ca2+]m values increased, whereas in Mg,ATP-medium statistically reliable changes were not registered. Preincubation of mitochondria with 10 μM Са2+ did not affect the [Ca2+]m value after the addition of 100 μM Са2+. The [Ca2+]m values after 100 μM Са2+ addition were the same in 0- and Mg,ATP-mediums and somewhat lower in Mg-medium. Preliminary incubation of mitochondria with 10 μM Са2+ in 0- and Mg-mediums reduced changes of Fluo 4 normalized fluorescence values that were induced by 100 μM Са2+ additions, but in Mg,ATP-medium such differences were not recorded. It is concluded that Са2+ exchange in myometrium mitochondria is regulated by the concentration of Ca ions as in the external medium, so in the matrix of mitochondria. The medium composition had a significant impact on the [Са2+]m values in the absence of exogenous cation. It is suggested that light increase of [Са2+]m before the addition of 100 μM Са2+ may have a positive effect on the functional activity of the mitochondria.

  17. Mg/Ca of Continental Ostracode Shells

    Science.gov (United States)

    Ito, E.; Forester, R. M.; Marco-Barba, J.; Mezquita, F.

    2007-12-01

    Marine ionic chemistry is thought to remain constant. This, together with the belief that marine calcifiers partition Mg/Ca in a systematic manner as functions of temperature (and Mg/Ca) of water forms the basis of the Mg/Ca thermometer. In continental settings both of these assumptions are usually not true. Continental waters contain a wide variety of solutes in absolute and relative ion concentrations. Hence, waters with identical Mg/Ca may have very different concentrations of Mg and Ca and very different anions. Here we use two examples to focus on the effects of ion chemistry on Mg/Ca partitioning in continental ostracode shells and we ignore the complexities of solute evolution, which can change Mg/Ca over timescales of minutes to millennia. Palacios-Fest and Dettman (2001) conducted a monthly study of ,Cypridopsis vidua at El Yeso Lake in Sonora, Mexico. They established a relation between temperature and average shell Mg/Ca using regression analyses on averaged data. When their Mg/Ca-temperature relation is applied to monthly ,C. vidua data from Page Pond near Cleveland, Ohio, water temperatures of -8 to -1°C are obtained. The observed Mg/Ca ranges for El Yeso Lake (0.31 to 0.46) and Page Pond (0.33 to 0.46) are similar, as are their specific conductivities (700 to 850μS for El Yeso Lake; 400 to 600μS for Page Pond). However, [Ca] is 140-260 mg/L for El Yeso, but only 70-90 mg/L for Page Pond. Page Pond data, in fact, shows a good temperature shell Mg/Ca relation for .C. vidua, but the relation is different from that at El Yeso. Hence, shell Mg/Ca is a multi-valued, family of curves function of temperature and Mg/Ca of water that depends on the [Mg] and [Ca] values in water and perhaps other factors. Our second example comes from sites near Valencia, Spain and involves shell data for ,Cyprideis torosa, an estuarine ostracode that is tolerant of a wide range of salinity and can live in continental waters as long as the carbonate alkalinity to Ca ratio is

  18. Electronic structures and magnetism of CaFeAsH and CaFeAsF

    International Nuclear Information System (INIS)

    Wang Guangtao; Shi Xianbiao; Liu Haipeng; Liu Qingbo

    2015-01-01

    We studied the electronic structures, magnetism, and Fermi surface (FS) nesting of CaFeAsH and CaFeAsF by first-principles calculations. In the nonmagnetic (NM) states, we found strong FS nesting, which induces magnetic instability and a spin density wave (SDW). Our calculations indicate that the ground state of CaFeAsH and CaFeAsF is the stripe antiferromagnetic state. The calculated bare susceptibility χ 0 (q) peaked at the M-point and was clearly suppressed and became slightly incommensurate with both electron doping and hole doping for both materials. (author)

  19. Effect of Ca content on equilibrium Ca isotope fractionation between orthopyroxene and clinopyroxene

    Science.gov (United States)

    Wang, Wenzhong; Zhou, Chen; Qin, Tian; Kang, Jin-Ting; Huang, Shichun; Wu, Zhongqing; Huang, Fang

    2017-12-01

    Concentration effect on equilibrium inter-mineral isotope fractionation is ubiquitous in solid solution systems, but it is not clear in which concentration range such effect is prominent. Using first-principles calculations, we examine the effect of Ca and Fe contents in orthopyroxene (opx) on its average Casbnd O bond length and the equilibrium Ca isotope fractionation factor (103lnα) between opx and clinopyroxene (cpx). Our results reveal that the average Casbnd O bond length in opx is much smaller than that in cpx and it does not change with variable Ca content x (x and y are mole ratios in CaxFeyMg1-x-ySiO3 thereafter here) when x ≤ 1/48. Incorporation of Fe (y ≥ 1/32) into opx with a fixed Ca content can only slightly increase the average Casbnd O bond length. 103lnαopx-cpx of 44Ca/40Ca is linearly correlated with the average Casbnd O bond length in opx, suggesting that 103lnαopx-cpx of 44Ca/40Ca is controlled by opx Casbnd O bond strength. Our calculations indicate that the Ca concentration effect on 103lnαopx-cpx is significant when x in opx ranges from 2/16 to 1/48, while Fe in natural opx only causes a slight decrease in 103lnαopx-cpx. Our results provide insights into Ca isotope fractionation in high-temperature geochemical processes. Given that Ca content x in opx from natural peridotites is usually lower than 1/32 and Fe content y is generally ∼10 mol%, Ca and Fe concentration effects on 103lnαopx-cpx in natural samples are negligible. Rather, 103lnαopx-cpx is mainly controlled by temperature. 103lnαopx-cpx of 44Ca/40Ca decreases from 0.50‰ to 0.26‰ when temperature increases from 1000 K to 1400 K if the Fe effect is taken into account. Therefore, if Ca isotope fractionation between opx and cpx (Δ44/40Caopx-cpx) in natural peridotites is greater than 0.50‰ or lower than 0.26‰, it may indicate disequilibrium of Ca isotopes. Finally, the large 103lnαopx-cpx relative to our current analytical precision suggests that Δ44/40Caopx

  20. Prognostic significance of preoperative serum CA125, CA19-9 and CEA in gastric carcinoma

    Science.gov (United States)

    Wang, Wei; Chen, Xiao-Long; Zhao, Shen-Yu; Xu, Yu-Hui; Zhang, Wei-Han; Liu, Kai; Chen, Xin-Zu; Yang, Kun; Zhang, Bo; Chen, Zhi-Xin; Chen, Jia-Ping; Zhou, Zong-Guang; Hu, Jian-Kun

    2016-01-01

    The prognostic significance of preoperative serum CA125, CA19-9 and CEA in gastric carcinoma (GC) has been widely reported and is still under debate. Here, we evaluated the prognostic significance of preoperative serum CA125, CA19-9 and CEA in patients with GC. 1692 patients with GC who underwent gastrectomy were divided into the training (from January 2005 to December 2011, n = 1024) and the validation (from January 2012 to December 2013, n = 668) cohorts. Positive groups of CA125 (> 13.72 U/ml), CA19-9 (> 23.36 U/ml) and CEA (> 4.28 ng/ml) were significantly associated with more advanced clinicopathological traits and worse outcomes than that of negative groups (all P tumor size (P tumor markers (NPTM) were more accurate in prognostic prediction than TNM stage alone. Our findings suggested that elevated preoperative serum CA125, CA19-9 and CEA were associated with more advanced clinicopathological traits and less favorable outcomes. In addition, CA125 as an independent prognostic factor should be further investigated. Nomogram based on NPTM could accurately predict the prognosis of GC patients. PMID:27097114

  1. Ca adsorption on dickite, Sericite and quartz. Dickite, sericite oyobi sekiei ni taisuru Ca no kyuchaku

    Energy Technology Data Exchange (ETDEWEB)

    Matsuoka, Isao; Nozawa, Miki (Tohoku Univ., Sendai, (Japan). Faculty of Engineering)

    1990-02-25

    Ca adsorbing characteristics of dickite were studied to clarify the possibility of clay minerals on selective Ca activation. Ca adsorption on hydrogenated dickite was compared with those of Na {sup +} and K {sup +} and further, the comparison was made for adsorption of Ca and these ikns on quartz and hydrogenated sericite. Ca adsorption on quartz which is thought to be caused by the negative charge of broken bond, depends strongly on pH and for pH of 7 or more, Ca acts strongly on any quartz surface. Moreover, when Na {sup +} or K {sup +} coexists, the effect of coexisting ion on Ca adsorption does not result any difference. On the contrary, Ca adsorption on sericite is mainly caused by the exchanging adsorption with H {sup +} and Ca saturated adsortion does not depend on pH in the range of 4-10pH. But Na {sup +} adsorption does not reach the saturation for lower pH unless Na {sup +} of sufficient concentration exists. 13 refs., 16 figs.

  2. Dielectron production in p+Be and Ca+Ca collisions at the Bevalac

    Energy Technology Data Exchange (ETDEWEB)

    Roche, G.; Krebs, G.F.; Lallier, E.; Letessier-Selvon, A.; Matis, H.S.; Naudet, C.; Schroeder, L.; Seidl, P.A.; Yegneswaran, A.; Bystricky, J.; Carroll, J.; Gordon, J.; Igo, G.; Hallman, T.; Madansky, L.; Welsh, R.; Kirk, P.; Wang, Z.F.; Miller, D.; Landaud, G.

    1988-06-01

    Dielectron production has been measured in p+Be collisions at 1.0, 2.1 and 4.9 GeV, and in Ca+Ca at 1.0 and 2.0 GeV/A. The observation of a structure in the mass cross section at about two times the pion mass suggests that pion annihilation is the dominant production mechanism in p+Be above 2 GeV. Comparison of preliminary results from p+Be and Ca+Ca at 1 GeV/A is presented. 7 refs., 5 figs., 1 tab.

  3. GPR surveying of transport infrastructures and buildings; underground utility and void sensing - ongoing activities in Working Group 2 of COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Plati, Christina; Derobert, Xavier

    2015-04-01

    This work aims at presenting the ongoing research activities carried out in Working Group 2 'GPR surveying of pavements, bridges, tunnels and buildings; underground utility and void sensing' of the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar' (www.GPRadar.eu). The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of Ground Penetrating Radar (GPR) techniques in civil engineering, whilst simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Four Working Groups (WGs) carry out the research activities. WG1 focuses on the development of innovative GPR equipment dedicated for civil engineering applications. WG2 deals with the development of guidelines and protocols for the surveying, through the use of a GPR system, of transport infrastructure and buildings, as well as for the sensing of utilities and voids. WG3 deals with the development of electromagnetic forward and inverse scattering methods, for the characterization of GPR scenarios, as well as with data- processing algorithms for the elaboration of the data collected during GPR surveys. WG4 is concerned with the use of GPR in fields different from the civil engineering, as well as with the integration of GPR with other non-destructive testing techniques. Each WG includes several Projects. WG2 includes five Projects. Project 2.1 focuses on outlining 'Innovative inspection procedures for effective GPR surveying of critical transport infrastructures (pavements, bridges and tunnels).' Project 2.2 is concerned with the development of 'Innovative inspection procedures for effective GPR surveying of buildings.' Project 2.3 deals with identifying 'Innovative inspection procedures for effective GPR sensing and mapping of underground utilities and voids, with a focus to urban

  4. Physical conditions in CaFe interstellar clouds

    OpenAIRE

    Gnacinski, P.; Krogulec, M.

    2007-01-01

    Interstellar clouds that exhibit strong Ca I and Fe I lines were called CaFe clouds. The ionisation equilibrium equations were used to model the column densities of Ca II, Ca I, K I, Na I, Fe I and Ti II in CaFe clouds. The chemical composition of CaFe clouds is that of the Solar System and no depletion of elements onto dust grains is seen. The CaFe clouds have high electron densities n=1 cm^-3 that leads to high column densities of neutral Ca and Fe.

  5. Absolute Ca Isotopic Measurement Using an Improved Double Spike Technique

    Directory of Open Access Journals (Sweden)

    Jason Jiun-San Shen

    2009-01-01

    Full Text Available A new vector analytical method has been developed in order to obtain the true isotopic composition of the 42Ca-48Ca double spike. This is achieved by using two different sample-spike mixtures combined with the double spike and natural Ca data. Be cause the natural sample (two mixtures and the spike should all lie on a single mixing line, we are able to con strain the true isotopic composition of our double spike using this new approach. Once the isotopic composition of the Ca double spike is established, we are able to obtain the true Ca isotopic composition of the NIST Ca standard SRM915a, 40Ca/44Ca = 46.537 ± 2 (2sm, n = 55, 42Ca/44Ca = 0.31031 ± 1, 43Ca/44Ca = 0.06474 ± 1, and 48Ca/44Ca = 0.08956 ± 1. De spite an off set of 1.3% in 40Ca/44Ca between our result and the previously re ported value (Russell et al. 1978, our data indicate an off set of 1.89__in 40Ca/44Ca between SRM915a and seawater, entirely consistent with the published results.

  6. The destiny of Ca(2+) released by mitochondria.

    Science.gov (United States)

    Takeuchi, Ayako; Kim, Bongju; Matsuoka, Satoshi

    2015-01-01

    Mitochondrial Ca(2+) is known to regulate diverse cellular functions, for example energy production and cell death, by modulating mitochondrial dehydrogenases, inducing production of reactive oxygen species, and opening mitochondrial permeability transition pores. In addition to the action of Ca(2+) within mitochondria, Ca(2+) released from mitochondria is also important in a variety of cellular functions. In the last 5 years, the molecules responsible for mitochondrial Ca(2+) dynamics have been identified: a mitochondrial Ca(2+) uniporter (MCU), a mitochondrial Na(+)-Ca(2+) exchanger (NCLX), and a candidate for a mitochondrial H(+)-Ca(2+) exchanger (Letm1). In this review, we focus on the mitochondrial Ca(2+) release system, and discuss its physiological and pathophysiological significance. Accumulating evidence suggests that the mitochondrial Ca(2+) release system is not only crucial in maintaining mitochondrial Ca(2+) homeostasis but also participates in the Ca(2+) crosstalk between mitochondria and the plasma membrane and between mitochondria and the endoplasmic/sarcoplasmic reticulum.

  7. Changes in the expression of serum markers CA242, CA199, CA125, CEA, TNF-α and TSGF after cryosurgery in pancreatic cancer patients.

    Science.gov (United States)

    Zhou, Gang; Niu, Lizhi; Chiu, David; He, Lihua; Xu, Kecheng

    2012-07-01

    The presence of serum tumor markers, carbohydrate antigen 242 (CA242), carbohydrate antigen 199 (CA199), carbohydrate antigen 125 (CA125), carcinoembryonic antigen (CEA), tumor-supplied group of factors (TSGF) and tumor necrosis factor-α (TNF-α), is closely associated with invasion and metastasis of many malignancies. The expression of these markers were measured in serum taken from 37 pancreatic cancer patients prior to treatment. Levels of CA242, CA199, CA125, CEA and TNF-α expression correlated with tumor size, clinical stage, tumor differentiation, lymph node and liver metastasis (P markers were significantly reduced compared with levels prior to cryosurgery (P 0.05). Thus, cryosurgery is more effective than chemotherapy for decreasing CA242, CA199, CA125, CEA, TSGF and TNF-α serum levels in these patients.

  8. Treatment of Ruptured Ovarian Endometrioma with Extremely High CA 125, Moderately High CA 19-9 and CA 15-3 Level

    Directory of Open Access Journals (Sweden)

    Muzeyyen Duran

    2013-06-01

    Full Text Available In this case report, a ruptured ovarian endometrioma with a very high CA-125 level, moderately elevated CA 19-9, and CA 15-3 levels is presented. A 20 years old patient, complaining from pelvic pain, 5 cm adnexial mass was detected on left side. Biochemical examination was revealed very high CA-125 value (2556IU/ml, moderately elevated CA 19-9 (134IU/ml, and CA 15-3 (65IU/ml values. Laparoscopy was done and a ruptured ovarian endometrioma of 5 cm was seen during operation. After the total excision of the cyst, tumor markers fell rapidly. Very high CA-125 value, moderately elevated CA19-9, and CA 15-3 values can be seen in cases with ruptured endometrioma. In young patients, endometrioma must be considered firstly and laparoscopy should be applied instead of more invasive methods unless there was any finding or strong suspicion about malignancy.

  9. Control of ciliary motility by Ca sup 2+ : Integration of Ca sup 2+ -dependent functions and targets for Ca sup 2+ action

    Energy Technology Data Exchange (ETDEWEB)

    Evans, T.C.

    1988-01-01

    To identify functions that regulate Ca{sup 2+}-induced ciliary reversal in Paramecium, mutants defective in terminating depolarization-induced backward swimming were selected. Six independent recessive mutations (k-shy) comprising two complementation groups, k-shyA and k-shyB, were identified. All mutants exhibited prolonged backward swimming in depolarizing solutions. Voltage clamp studies revealed that mutant Ca{sup 2+} current amplitudes were reduced, but could be restored to wild type levels by EGTA injection. The recovery of the mutant Ca{sup 2+} current from Ca{sup 2+}-dependent inactivation, and the decay of the Ca{sup 2+}-dependent K{sup +} and Ca{sup 2+}-dependent Na{sup +} currents after depolarization were slow in k-shy compared to wild type. To identify protein targets of Ca{sup 2+} action, ciliary proteins that interact with calmodulin (CaM) were characterized. With a {sup 125}I-CaM blot assay, several CaM-binding proteins were identified including axonemal, soluble, and membrane-bound polypeptides. Competitive displacement studies with unlabeled Paramecium CaM, bovine CaM, and troponinC suggested that both protein types bind CaM with high affinity and specificity. To examine the presence of CaM-binding sites in intact axonemes, a filtration binding assay was developed.

  10. Modeling the contributions of Ca2+ flows to spontaneous Ca2+ oscillations and cortical spreading depression-triggered Ca2+ waves in astrocyte networks.

    Directory of Open Access Journals (Sweden)

    Bing Li

    Full Text Available Astrocytes participate in brain functions through Ca(2+ signals, including Ca(2+ waves and Ca(2+ oscillations. Currently the mechanisms of Ca(2+ signals in astrocytes are not fully clear. Here, we present a computational model to specify the relative contributions of different Ca(2+ flows between the extracellular space, the cytoplasm and the endoplasmic reticulum of astrocytes to the generation of spontaneous Ca(2+ oscillations (CASs and cortical spreading depression (CSD-triggered Ca(2+ waves (CSDCWs in a one-dimensional astrocyte network. This model shows that CASs depend primarily on Ca(2+ released from internal stores of astrocytes, and CSDCWs depend mainly on voltage-gated Ca(2+ influx. It predicts that voltage-gated Ca(2+ influx is able to generate Ca(2+ waves during the process of CSD even after depleting internal Ca(2+ stores. Furthermore, the model investigates the interactions between CASs and CSDCWs and shows that the pass of CSDCWs suppresses CASs, whereas CASs do not prevent the generation of CSDCWs. This work quantitatively analyzes the generation of astrocytic Ca(2+ signals and indicates different mechanisms underlying CSDCWs and non-CSDCWs. Research on the different types of Ca(2+ signals might help to understand the ways by which astrocytes participate in information processing in brain functions.

  11. Ventral tegmental area disruption selectively affects CA1/CA2 but not CA3 place fields during a differential reward working memory task.

    Science.gov (United States)

    Martig, Adria K; Mizumori, Sheri J Y

    2011-02-01

    Hippocampus (HPC) receives dopaminergic (DA) projections from the ventral tegmental area (VTA) and substantia nigra. These inputs appear to provide a modulatory signal that influences HPC dependent behaviors and place fields. We examined how efferent projections from VTA to HPC influence spatial working memory and place fields when the reward context changes. CA1 and CA3 process environmental context changes differently and VTA preferentially innervates CA1. Given these anatomical data and electrophysiological evidence that implicate DA in reward processing, we predicted that CA1 place fields would respond more strongly to both VTA disruption and changes in the reward context than CA3 place fields. Rats (N = 9) were implanted with infusion cannula targeting VTA and recording tetrodes aimed at HPC. Then they were tested on a differential reward, win-shift working memory task. One recording session consisted of 5 baseline and 5 manipulation trials during which place cells in CA1/CA2 (N = 167) and CA3 (N = 94) were recorded. Prior to manipulation trials rats were infused with either baclofen or saline and then subjected to control or reward conditions during which the learned locations of large and small reward quantities were reversed. VTA disruption resulted in an increase in errors, and in CA1/CA2 place field reorganization. There were no changes in any measures of CA3 place field stability during VTA disruption. Reward manipulations did not affect performance or place field stability in CA1/CA2 or CA3; however, changes in the reward locations "rescued" performance and place field stability in CA1/CA2 when VTA activity was compromised, perhaps by trigging compensatory mechanisms. These data support the hypothesis that VTA contributes to spatial working memory performance perhaps by maintaining place field stability selectively in CA1/CA2. Copyright © 2009 Wiley-Liss, Inc.

  12. Adverse bioenergetic consequences of Na+-Ca2+ exchanger-mediated Ca2+ influx in cardiac myocytes.

    Science.gov (United States)

    Kohlhaas, Michael; Maack, Christoph

    2010-11-30

    In heart failure, the Na+-Ca²+ exchanger (NCX) is upregulated and mediates Ca²+ influx (instead of efflux) during the cardiac action potential. Although this partly compensates for impaired sarcoplasmic reticulum Ca²+ release and supports inotropy, the energetic consequences have never been considered. Because NCX-mediated Ca²+ influx is rather slow and mitochondrial Ca²+ uptake (which stimulates NADH production by the Krebs cycle) is thought to be facilitated by high Ca²+ gradients in a "mitochondrial Ca²+ microdomain," we speculated that NCX-mediated Ca²+ influx negatively affects the bioenergetic feedback response. Methods and Results- With the use of a patch-clamp-based approach in guinea-pig myocytes, cytosolic and mitochondrial Ca²+ ([Ca²+](c) and [Ca²+](m), respectively) was determined within the same cell after varying Ca²+ influx via L-type Ca²+ channels (I(Ca,L)) or the NCX. The efficiency of mitochondrial Ca²+ uptake, indexed by the slope of plotting [Ca²+](m) against [Ca²+](c) during each Ca²+ transient, was maximal during I(Ca,L)-triggered sarcoplasmic reticulum Ca²+ release. Depletion of sarcoplasmic reticulum Ca²+ load and increased contribution of the NCX to cytosolic Ca²+ influx independently reduced the efficiency of mitochondrial Ca²+ uptake. The upstroke velocity of cytosolic Ca²+ transients closely correlated with the efficiency of mitochondrial Ca²+ uptake. Despite comparable [Ca²+](c), sarcoplasmic reticulum Ca²+ release, but not NCX-mediated Ca²+ influx, led to stimulation of Ca²+-sensitive dehydrogenases of the Krebs cycle. Conclusions- Increased contribution of the NCX to cytosolic Ca²+ transients, which occurs in cardiac myocytes from failing hearts, impairs mitochondrial Ca²+ uptake and the bioenergetic feedback response. This mechanism could contribute to energy starvation of failing hearts.

  13. Comparative study of structural, electronic and optical properties of Ca2Si, Ca2Ge, Ca2Sn, and Ca2Pb

    Science.gov (United States)

    Migas, D. B.; Miglio, Leo; Shaposhnikov, V. L.; Borisenko, V. E.

    2003-05-01

    We report ab initio results for the ground-state properties, band diagrams, density of states, and dielectric functions of Ca2Z (Z=Si, Ge, Sn, and Pb) both in the orthorhombic and cubic structures. The calculations are performed by means of total energy ultrasoft pseudopotential and full potential linearized augmented plane wave methods within local density and generalized gradient approximations. The estimated difference in the cohesion energy shows the orthorhombic structure to be the stable phase. We also demonstrate that these materials are semiconductors independently of the phase. A direct energy gap and sizable anisotropy in optical spectra characterize orthorhombic Ca2Z, whereas a competitive direct-indirect character of the gap and a high value of oscillator strength of the first direct transition are predicted for cubic Ca2Z.

  14. Synaptotagmin-7 is a principal Ca2+ sensor for Ca2+ -induced glucagon exocytosis in pancreas

    DEFF Research Database (Denmark)

    Gustavsson, Natalia; Wei, Shun-Hui; Hoang, Dong Nhut

    2009-01-01

    Hormones such as glucagon are secreted by Ca(2+)-induced exocytosis of large dense-core vesicles, but the mechanisms involved have only been partially elucidated. Studies of pancreatic beta-cells secreting insulin revealed that synaptotagmin-7 alone is not sufficient to mediate Ca(2+)-dependent...... insulin granule exocytosis, and studies of chromaffin cells secreting neuropeptides and catecholamines showed that synaptotagmin-1 and -7 collaborate as Ca(2+) sensors for exocytosis, and that both are equally involved. As no other peptide secretion was analysed, it remains unclear whether synaptotagmins...... generally act as Ca(2+) sensors in large dense-core vesicle exocytosis in endocrine cells, and if so, whether synaptotagmin-7 always functions with a partner in that role. In particular, far less is known about the mechanisms underlying Ca(2+)-triggered glucagon release from alpha-cells than insulin...

  15. Study on Ca2+ antagonistic effect and mechanism of Chinese herbal drugs using 45Ca

    International Nuclear Information System (INIS)

    Yang Yuanyou; Liu Ning; Mo Shangwu; Qiu Mingfeng; Jin Jiannan; Liao Jiali

    2002-01-01

    The Ca 2+ antagonistic effect and mechanism of Chinese herbal drugs are studied by using 45 Ca. The results indicate that potential-dependent Ca 2+ channel (PDC) and receptor-operated Ca 2+ channel (ROC) in cell membranes of smooth muscle can be blocked by several Chinese herbal drugs, including as Crocus sativus L., Carthamus L., Di-ao-xin-xue-kang (DAXXG) and Ginkgo biloba L. leaves. Among them Crocus sativus L. has the strongest antagonistic effect on Ca 2+ channel, while Ginkgo biloba L. leaves has no obvious effect. The whole prescription and the other functional drugs have significant effect on ROC and PDC. The compositions extracted by hexane have the strongest antagonistic. The wrinkled giant hyssop have five active compositions and Pei-lan have two active compositions

  16. Applicative Value of Serum CA19-9, CEA, CA125 and CA242 in Diagnosis and Prognosis for Patients with Pancreatic Cancer Treated by Concurrent Chemoradiotherapy.

    Science.gov (United States)

    Gu, Yu-Lei; Lan, Chao; Pei, Hui; Yang, Shuang-Ning; Liu, Yan-Fen; Xiao, Li-Li

    2015-01-01

    To evaluate the application value of serum CA19-9, CEA, CA125 and CA242 in diagnosis and prognosis of pancreatic cancer cases treated with concurrent chemotherapy. 52 patients with pancreatic cancer, 40 with benign pancreatic diseases and 40 healthy people were selected. The electrochemiluminescence immunoassay method was used for detecting levels of CA19-9, CEA and CA125, and a CanAg CA242 enzyme linked immunoassay kit for assessing the level of CA242. The Kaplan-Meier method was used for analyzing the prognostic factors of patients with pancreatic cancer. The Cox proportional hazard model was applied for analyzing the hazard ratio (HR) and 95% confidential interval (CI) for survival time of patients with pancreatic cancer. The levels of serum CA19-9, CEA, CA125 and CA242 in patients with pancreatic cancer were significantly higher than those in patients with benign pancreatic diseases and healthy people (PCEA. The specificity of CA242 is the highest, followed by CA125, CEA and CA19-9. The sensitivity and specificity of joint detection of serum CA19-9, CEA, CA125and CA242 were 90.4% and 93.8%, obviously higher than single detection of those markers in diagnosis of pancreatic cancer. The median survival time of 52 patients with pancreatic cancer was 10 months (95% CI7.389~12.611).. Patients with the increasing level of serum CA19-9, CEA, CA125, CA242 had shorter survival times (P=0.047. 0.043, 0.0041, 0.029). COX regression analysis showed that CA19-9 was an independent prognostic factor for patients with pancreatic cancer (P=0.001, 95%CI 2.591~38.243). The detection of serum tumor markers (CA19.9, CEA, CA125 and CA242) is conducive to the early diagnosis of pancreatic cancer and joint detection of tumor markers helps improve the diagnostic efficiency. Moreover, CA19-9 is an independent prognostic factor for patients with pancreatic cancer.

  17. Measurements of hyperfine constants in Ca+

    International Nuclear Information System (INIS)

    Goble, A.T.; Maleki, S.

    1990-01-01

    We have measured the hyperfine constants A( 2 S 1/2 ), A( 2 P 1/2 ), and A( 2 P 3/2 ) for Ca + . We compare our results with the calculations of Martensson-Pendrill and Salomonson [Phys. Rev. A 30, 712 (1984)], who used many-body perturbation theory (MBPT). The agreement between our results and MBPT calculations for Ca + is about 3%, which is comparable to similar comparisons reported for Cs [S. Blundell, in 2 Relativistic, Quantum Electrodynamic, and Weak Interaction Effects in Atoms, AIP Conf. Proc. No. 189, edited by Walter Johnson, Peter Mohr, and Joseph Sucher (AIP, New York, 1988)

  18. Divdaivu Ginka (Ginkgo Biloba) farmakoloģiskās īpašības un tā preparātu aprite SIA "Gulbju aptiekā"

    OpenAIRE

    Indriča, Alīna

    2014-01-01

    Divdaivu ginks (Ginkgo biloba) ir viens no vecākajiem kokiem pasaulē. Ķīnas tradicionālajā medicīnā ginka lapas un sēklas izmanto jau vismaz 5000 gadus. Standartizēts Ginkgo biloba ekstrakts satur – 24% flavonglikozīdu un 6% terpēnlaktonu. Šī darba mērķis ir veikt apkopoto datu analīzi par Ginkgo biloba preparātu apriti „Gulbju aptiekā” – Gulbenē, 2013. gada laikā, kā arī noskaidrot cilvēku informētību par Ginkgo biloba preparātu lietošanu. Darbā tika izvirzīti 4 uzdevumi: Noskaidrot, k...

  19. Depresijas, traumatiskās pieredzes un disociācijas saistība pacientiem ar diagnosticētu depresiju un psihiski veseliem cilvēkiem

    OpenAIRE

    Bērtule, Marika

    2014-01-01

    Pētījuma mērķis: noteikt kāda saistība pastāv starp depresijas izteiktības pakāpi, traumatisko pieredzi un disociatīvo traucējumu simptomu līmeni. Pētījuma dalībnieki: grupa ar diagnosticētu depresiju - 36 pacienti. Kontroles grupa – 58 personas. Instrumentārijs: Beka depresijas izpētes aptauja (BDI-II), Traumatisko pagātnes notikumu aptauja (TAQ), Disociācijas Multidimensiju Aptauja (MID-6). Rezultāts: grupā ar diagnosticētu depresiju depresijas izteiktības, psihotraumatiskās piered...

  20. The hippocampal CA2 ensemble is sensitive to contextual change.

    Science.gov (United States)

    Wintzer, Marie E; Boehringer, Roman; Polygalov, Denis; McHugh, Thomas J

    2014-02-19

    Contextual learning involves associating cues with an environment and relating them to past experience. Previous data indicate functional specialization within the hippocampal circuit: the dentate gyrus (DG) is crucial for discriminating similar contexts, whereas CA3 is required for associative encoding and recall. Here, we used Arc/H1a catFISH imaging to address the contribution of the largely overlooked CA2 region to contextual learning by comparing ensemble codes across CA3, CA2, and CA1 in mice exposed to familiar, altered, and novel contexts. Further, to manipulate the quality of information arriving in CA2 we used two hippocampal mutant mouse lines, CA3-NR1 KOs and DG-NR1 KOs, that result in hippocampal CA3 neuronal activity that is uncoupled from the animal's sensory environment. Our data reveal largely coherent responses across the CA axis in control mice in purely novel or familiar contexts; however, in the mutant mice subject to these protocols the CA2 response becomes uncoupled from CA1 and CA3. Moreover, we show in wild-type mice that the CA2 ensemble is more sensitive than CA1 and CA3 to small changes in overall context. Our data suggest that CA2 may be tuned to remap in response to any conflict between stored and current experience.

  1. Electrochemical formation of Mg-Li-Ca alloys by codeposition of Mg, Li and Ca from LiCl-KCl-MgCl2-CaCl2 melts.

    Science.gov (United States)

    Yan, Yong De; Zhang, Mi Lin; Xue, Yun; Han, Wei; Cao, Dian Xue; Jing, Xiao Yan; He, Li Yi; Yuan, Yi

    2009-08-07

    This work presents electrochemical formation of Mg-Li-Ca alloys via codeposition of Mg, Li and Ca on a molybdenum electrode in KCl-LiCl-MgCl(2)-CaCl(2) melts at 943 K. Cyclic voltammograms (CVs) showed that the underpotential deposition (UPD) of calcium on pre-deposited magnesium leads to the formation of a liquid Mg-Ca alloy, and the succeeding underpotential deposition of lithium on pre-deposited Mg-Ca alloy leads to the formation of a liquid Mg-Li-Ca solution. Chronopotentiometric measurements indicated that the codepositon of Mg, Li and Ca occurs at current densities more negative than -0.31 A cm(-2) in LiCl-KCl-MgCl(2) (5 wt%) melts containing 1 wt% CaCl(2). Chronoamperograms demonstrated that the onset potential for the codeposition of Mg, Li and Ca is -2.200 V, and the codeposition of Mg, Li and Ca is formed when the applied potentials are more negative than -2.200 V. X-Ray diffraction (XRD) indicated that Mg-Li-Ca alloys with different phases were formed via galvanostatic electrolysis. The microstructures of typical alpha and beta phases of Mg-Li-Ca alloys were characterized by optical microscope (OM) and scanning electron microscopy (SEM). The analysis of energy dispersive spectrometry (EDS) showed that the element Ca mainly distributes along grain boundary in Mg-Li-Ca alloys. The results of inductively coupled plasma analysis determined that the chemical compositions of Mg-Li-Ca alloys correspond with the phase structures of XRD patterns, and the lithium and calcium contents of Mg-Li-Ca alloys depend on the concentrations of MgCl(2) and CaCl(2).

  2. Synergism between the GTPase activities of EF-Tu.GTP and EF-G.GTP on empty ribosomes. Elongation factors as stimulators of the ribosomal oscillation between two conformations.

    Science.gov (United States)

    Mesters, J R; Potapov, A P; de Graaf, J M; Kraal, B

    1994-10-07

    A remarkable positive cooperativity between the GTPase activities of EF-Tu and EF-G on empty ribosomes from Escherichia coli has been discovered. This cooperativity implies a decrease of the corresponding apparent KM values of the empty ribosome for either elongation factor: from more than 10 microM to 0.5 microM for EF-Tu.GTP by the addition of 0.25 microM EF-G and from 0.7 microM to 0.5 microM for EF-G.GTP by the addition of 3 microM EF-Tu. In a further analysis of this phenomenon, the effects of various specific antibiotics were studied: thiostrepton, fusidic acid, tetracycline, pulvomycin and kirromycin appeared to inhibit the synergistic effect, whereas streptomycin was found to stimulate it. Even in the present minimal system the ribosomes respond to the above-mentioned antibiotics in a way surprisingly similar to that in the coupled system with mRNA and tRNAs. The cooperativity seems not to be due to a simultaneous binding of the two elongation factors to the ribosome as revealed by studying the effects of fusidic acid and kirromycin, and by band-shift experiments by means of gel electrophoresis under non-denaturing conditions. Our experimental data and the kinetic analysis of alternative models provide evidence that EF-Tu.GTP and EF-G.GTP interact sequentially with empty ribosomes that oscillate between two different conformations, one for each elongation factor. Apparently, ribosomes have an intrinsic property for oscillation as normally observed during protein synthesis with a frequency paced by the events of tRNA binding and translocation.

  3. Design and testing of Ground Penetrating Radar equipment dedicated for civil engineering applications: ongoing activities in Working Group 1 of COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Manacorda, Guido; Persico, Raffaele

    2015-04-01

    This work aims at presenting the ongoing research activities carried out in Working Group 1 'Novel GPR instrumentation' of the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar' (www.GPRadar.eu). The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Working Group 1 (WG1) of the Action focuses on the development of innovative GPR equipment dedicated for civil engineering applications. It includes three Projects. Project 1.1 is focused on the 'Design, realisation and optimisation of innovative GPR equipment for the monitoring of critical transport infrastructures and buildings, and for the sensing of underground utilities and voids.' Project 1.2 is concerned with the 'Development and definition of advanced testing, calibration and stability procedures and protocols, for GPR equipment.' Project 1.3 deals with the 'Design, modelling and optimisation of GPR antennas.' During the first year of the Action, WG1 Members coordinated between themselves to address the state of the art and open problems in the scientific fields identified by the above-mentioned Projects [1, 2]. In carrying our this work, the WG1 strongly benefited from the participation of IDS Ingegneria dei Sistemi, one of the biggest GPR manufacturers, as well as from the contribution of external experts as David J. Daniels and Erica Utsi, sharing with the Action Members their wide experience on GPR technology and methodology (First General Meeting, July 2013). The synergy with WG2 and WG4 of the Action was useful for a deep understanding of the problems, merits and limits of available GPR equipment, as well as to discuss how to quantify the reliability of GPR results. An

  4. Value of Combined Detection of Serum CEA, CA72-4, CA19-9, CA15-3 and CA12-5 in the Diagnosis of Gastric Cancer.

    Science.gov (United States)

    Chen, Changguo; Chen, Qiuyuan; Zhao, Qiangyuan; Liu, Min; Guo, Jianwei

    2017-05-01

    To examine whether the combined detection of serum tumor markers (CEA, CA72-4, CA19-9, CA15-3 and CA12-5) improves the sensitivity and accuracy in the diagnosis of gastric cancer (GC). An automatic chemiluminescence immune analyzer with matched kits was used to determine the levels of serum CEA, CA72-4, CA19-9, CA15-3, and CA12-5 in 87 patients with gastric cancer (GC group), 60 patients with gastric benign diseases (GBD group) who were hospitalized during the same period, and 40 healthy subjects undergoing a physical examination. The values of these 5 tumor markers in the diagnosis of gastric cancer were analyzed. The levels of serum CEA, CA72-4, CA19-9, and CA12-5 were higher in the GC group than in the GBD group and healthy subjects, and these differences were significant ( P 0.05). The combined detection of CEA, CA72-4, CA19-9, and CA12-5 had a higher diagnostic value for gastric cancer than did single detection, and the positive detection rate of the combined detection of the four tumor markers was 60.9%. The diagnostic power when using the combined detection of CA72-4, CEA, CA19-9, and CA12-5 was the best. The combined detection of serum CA72-4, CEA, CA19-9 and CA12-5 increases the sensitivity and accuracy in the diagnosis of GC and can thus be considered an important tool for early diagnosis. © 2017 by the Association of Clinical Scientists, Inc.

  5. Ca and Mg binding induce conformational stability of Calfumirin-1 ...

    Indian Academy of Sciences (India)

    loops of CaBPs to accommodate the hexagonally coor- dinated metal ion.19–24 The different ionic radii of the two ions (Ca2+ = 1.14 Å and Mg2+ = 0.86 Å) enable specificity of CaBPs towards Ca2+ over Mg2+ binding, with distinguished affinity towards CaBPs.23 As there is a high concentration of Mg2+ in the cell, the bind-.

  6. Towards ultracold RbCa molecules

    Science.gov (United States)

    Kleinert, Michaela; Whitson, Hayley; Parsagian, Alexandria

    2012-06-01

    Ultracold heteronuclear molecules have seen increasing interest in the scientific community over the last few years. By controlling their ro-vibrational energy levels, ultracold molecules can be used for high precision spectroscopy, to study cold collisions with rich internal dynamics, as model systems for condensed matter physics, and as qubits in quantum information processing. We study the novel combination RbCa. In addition to a permanent electric dipole moment, it also possesses a permanent magnetic dipole moment. This makes it an ideal candidate to study strong long-range dipole-dipole interactions. We are currently in the process of adding a Ca MOT to our existing Rb MOT and will discuss our current and future efforts toward our goal of creating, for the first time, ultracold RbCa molecules. Molecules, once created, will be detected through resonantly enhanced multi-photon ionization (REMPI). We have also performed ab initio calculations to determine the electronic energy levels of RbCa, and calculated Franck-Condon factors between the ground and several excited states

  7. Ca teos report in register N 13377

    International Nuclear Information System (INIS)

    Pena, S; Arrighetti, R.

    2011-01-01

    This work is about a report about ca teos carried out in register N 13377 in the department of Canelones to know the depth of the field. The drilling done allowed to identify granite and clay. This granite outcrops are developed in the east of the middle course of the stream Pando

  8. literacy.ca EXPRESS. April 2010

    Science.gov (United States)

    Movement for Canadian Literacy, 2010

    2010-01-01

    This issue of "literacy.ca EXPRESS" focuses on poverty. The articles included in this issue are: (1) Poverty Overview; (2) Tony's Story; (3) LAN (Learner Advisory Network) Member's Story (Dianne Smith); (4) Linking Adult Literacy to Poverty Reduction; (5) MCL (Movement for Canadian Literacy) Update; (6) Highlights from the LAN; (7) Good…

  9. Elastic and inelastic scattering of 1.044 GeV protons by 40Ca, 42Ca, 44Ca, 48Ca and 48Ti

    International Nuclear Information System (INIS)

    Alkhazov, G.D.; Bauer, T.; Beurtey, R.; Boudard, A.; Bruge, G.; Chaumeaux, A.; Couvert, P.; Cvijanovich, G.; Duhm, H.H.; Fontaine, J.M.; Garreta, D.; Kulikov, A.V.; Legrand, D.; Lugol, J.C.; Saudinos, J.; Thirion, J.; Vorobyov, A.A.

    1976-01-01

    Elastic and inelastic scattering of 1.044 GeV protons have been studied on isotopically enriched even 40 42 48 48 Ca isotopes and 48 Ti. A spin independent Glauber theory analysis of the elastic scattering allowed the extraction of neutron and nuclear matter densities for these targets. (Auth.)

  10. Thapsigargin, a tumor promoter, discharges intracellular Ca2+ stores by specific inhibition of the endoplasmic reticulum Ca2(+)-ATPase

    DEFF Research Database (Denmark)

    Thastrup, Ole; Cullen, P J; Drøbak, B K

    1990-01-01

    + in sensitive cells by an acute and highly specific arrest of the endoplasmic reticulum Ca2+ pump, followed by a rapid Ca2+ leak from at least two pharmacologically distinct Ca2+ stores. The implications of this mechanism of action for the application of thapsigargin in the analysis of Ca2+ homeostasis...

  11. ASteCA: Automated Stellar Cluster Analysis

    Science.gov (United States)

    Perren, G. I.; Vázquez, R. A.; Piatti, A. E.

    2015-04-01

    We present the Automated Stellar Cluster Analysis package (ASteCA), a suit of tools designed to fully automate the standard tests applied on stellar clusters to determine their basic parameters. The set of functions included in the code make use of positional and photometric data to obtain precise and objective values for a given cluster's center coordinates, radius, luminosity function and integrated color magnitude, as well as characterizing through a statistical estimator its probability of being a true physical cluster rather than a random overdensity of field stars. ASteCA incorporates a Bayesian field star decontamination algorithm capable of assigning membership probabilities using photometric data alone. An isochrone fitting process based on the generation of synthetic clusters from theoretical isochrones and selection of the best fit through a genetic algorithm is also present, which allows ASteCA to provide accurate estimates for a cluster's metallicity, age, extinction and distance values along with its uncertainties. To validate the code we applied it on a large set of over 400 synthetic MASSCLEAN clusters with varying degrees of field star contamination as well as a smaller set of 20 observed Milky Way open clusters (Berkeley 7, Bochum 11, Czernik 26, Czernik 30, Haffner 11, Haffner 19, NGC 133, NGC 2236, NGC 2264, NGC 2324, NGC 2421, NGC 2627, NGC 6231, NGC 6383, NGC 6705, Ruprecht 1, Tombaugh 1, Trumpler 1, Trumpler 5 and Trumpler 14) studied in the literature. The results show that ASteCA is able to recover cluster parameters with an acceptable precision even for those clusters affected by substantial field star contamination. ASteCA is written in Python and is made available as an open source code which can be downloaded ready to be used from its official site.

  12. Effects of Ca on microstructure, mechanical and corrosion properties and biocompatibility of Mg-Zn-Ca alloys.

    Science.gov (United States)

    Yin, Ping; Li, Nian Feng; Lei, Ting; Liu, Lin; Ouyang, Chun

    2013-06-01

    Zn and Ca were selected as alloying elements to develop an Mg-Zn-Ca alloy system for biomedical application due to their good biocompatibility. The effects of Ca on the microstructure, mechanical and corrosion properties as well as the biocompatibility of the as-cast Mg-Zn-Ca alloys were studied. Results indicate that the microstructure of Mg-Zn-Ca alloys typically consists of primary α-Mg matrix and Ca₂Mg₆Zn₃/Mg₂Ca intermetallic phase mainly distributed along grain boundary. The yield strength of Mg-Zn-Ca alloy increased slightly with the increase of Ca content, whilst its tensile strength increased at first and then decreased. Corrosion tests in the simulated body fluid revealed that the addition of Ca is detrimental to corrosion resistance due to the micro-galvanic corrosion acceleration. In vitro hemolysis and cytotoxicity assessment disclose that Mg-5Zn-1.0Ca alloy has suitable biocompatibility.

  13. Mg and Ca isotope fractionation during CaCO3 biomineralisation.

    Science.gov (United States)

    Chang, Veronica T-C; Williams, R J P; Makishima, Akio; Belshawl, Nick S; O'Nions, R Keith

    2004-10-08

    The natural variation of Mg and Ca stable isotopes of carbonates has been determined in carbonate skeletons of perforate foraminifera and reef coral together with Mg/Ca ratios to assess the influence of biomineralisation processes. The results for coral aragonite suggest its formation, in terms of stable isotope behaviour, approximates to inorganic precipitation from a seawater reservoir. In contrast, results for foraminifera calcite suggest a marked biological control on Mg isotope ratios presumably related to its low Mg content compared with seawater. The bearing of these observations on the use of Mg and Ca isotopes as proxies in paleoceanography is considered. Copyright 2004 Elsevier Inc.

  14. Reassigning the CaH+ 11Σ → 21Σ vibronic transition with CaD+

    Science.gov (United States)

    Condoluci, J.; Janardan, S.; Calvin, A. T.; Rugango, R.; Shu, G.; Sherrill, C. D.; Brown, K. R.

    2017-12-01

    We observe vibronic transitions in CaD+ between the 11Σ and 21Σ electronic states by resonance enhanced multiphoton photodissociation spectroscopy in a Coulomb crystal. The vibronic transitions are compared with previous measurements on CaH+. The result is a revised assignment of the CaH+ vibronic levels and a disagreement with multi-state-complete-active-space second-order perturbation theory theoretical calculations by approximately 700 cm-1. Updated high-level coupled-cluster calculations that include core-valence correlations reduce the disagreement between theory and experiment to 300 cm-1.

  15. Interlaboratory study for coral Sr/Ca and other element/Ca ratio measurements

    OpenAIRE

    Hathorne, Ed C.; Gagnon, Alex; Felis, Thomas; Adkins, Jess; Asami, Ryuji; Boer, Wim; Caillon, Nicolas; Case, David; Cobb, Kim M.; Douville, Eric; deMenocal, Peter; Eisenhauer, Anton; Garbe-Schönberg, Dieter; Geibert, Walter; Goldstein, Steven L.

    2013-01-01

    The Sr/Ca ratio of coral aragonite is used to reconstruct past sea surface temperature (SST). Twenty-one laboratories took part in an interlaboratory study of coral Sr/Ca measurements. Results show interlaboratory bias can be significant, and in the extreme case could result in a range in SST estimates of 7 degrees C. However, most of the data fall within a narrower range and the Porites coral reference material JCp-1 is now characterized well enough to have a certified Sr/Ca value of 8.838 m...

  16. Thermoluminescence of CaCO3:Dy and CaCO3:Mn

    International Nuclear Information System (INIS)

    Bapat, V.N.; Nambi, K.S.V.

    1976-01-01

    CaCO 3 samples doped with Dy and Mn were prepared in the laboratory by co-precipitation techniques. Thermoluminescence and emission spectra of these phosphors were studied and were compared with those of the naturally occuring calcite and undoped CaCO 3 samples. Dy-doping seems to give a more efficient phosphor and indicates a possibility of getting a better phosphor by a judicious choice of a rare earth doping of CaCO 3 . Interesting result have been obtained on the TL glow curve variations of these phosphors with different temperature treatments prior to irradiation. (author)

  17. Extra-matrix Mg2+ limits Ca2+ uptake and modulates Ca2+ uptake-independent respiration and redox state in cardiac isolated mitochondria

    NARCIS (Netherlands)

    Boelens, Age D.; Pradhan, Ranjan K.; Blomeyer, Christoph A.; Camara, Amadou K. S.; Dash, Ranjan K.; Stowe, David F.

    2013-01-01

    Cardiac mitochondrial matrix (m) free Ca(2+) ([Ca(2+)]m) increases primarily by Ca(2+) uptake through the Ca(2+) uniporter (CU). Ca(2+) uptake via the CU is attenuated by extra-matrix (e) Mg(2+) ([Mg(2+)]e). How [Ca(2+)]m is dynamically modulated by interacting physiological levels of [Ca(2+)]e and

  18. Serum concentrations of the biomarkers CA125, CA15-3, CA72-4, tPSA and PAPP-A in natural and stimulated ovarian cycles

    Directory of Open Access Journals (Sweden)

    Melissa Stemp

    2014-06-01

    Conclusion: Ovarian stimulation reduced serum PAPP‐A levels, CA125 and CA15‐3 levels were generally unaffected by ovarian stimulation but displayed cyclical changes throughout both natural and stimulated cycles, whilst tPSA and CA72-4 were not affected by the stage of the cycle or ovarian stimulation.

  19. Hydrogen peroxide inhibits Ca2+efflux through plasma membrane Ca2+-ATPase in mouse parotid acinar cells.

    Science.gov (United States)

    Kim, Min Jae; Choi, Kyung Jin; Yoon, Mi Na; Oh, Sang Hwan; Kim, Dong Kwan; Kim, Se Hoon; Park, Hyung Seo

    2018-03-01

    Intracellular Ca 2+ mobilization is closely linked with the initiation of salivary secretion in parotid acinar cells. Reactive oxygen species (ROS) are known to be related to a variety of oxidative stress-induced cellular disorders and believed to be involved in salivary impairments. In this study, we investigated the underlying mechanism of hydrogen peroxide (H 2 O 2 ) on cytosolic Ca 2+ accumulation in mouse parotid acinar cells. Intracellular Ca 2+ levels were slowly elevated when 1 mM H 2 O 2 was perfused in the presence of normal extracellular Ca 2+ . In a Ca 2+ -free medium, 1 mM H 2 O 2 still enhanced the intracellular Ca 2+ level. Ca 2+ entry tested using manganese quenching technique was not affected by perfusion of 1 mM H 2 O 2 . On the other hand, 10 mM H 2 O 2 induced more rapid Ca 2+ accumulation and facilitated Ca 2+ entry from extracellular fluid. Ca 2+ refill into intracellular Ca 2+ store and inositol 1,4,5-trisphosphate (1 µM)-induced Ca 2+ release from Ca 2+ store was not affected by 1 mM H 2 O 2 in permeabilized cells. Ca 2+ efflux through plasma membrane Ca 2+ -ATPase (PMCA) was markedly blocked by 1 mM H 2 O 2 in thapsigargin-treated intact acinar cells. Antioxidants, either catalase or dithiothreitol, completely protected H 2 O 2 -induced Ca 2+ accumulation through PMCA inactivation. From the above results, we suggest that excessive production of H 2 O 2 under pathological conditions may lead to cytosolic Ca 2+ accumulation and that the primary mechanism of H 2 O 2 -induced Ca 2+ accumulation is likely to inhibit Ca 2+ efflux through PMCA rather than mobilize Ca 2+ ions from extracellular medium or intracellular stores in mouse parotid acinar cells.

  20. Ca2+ alternans in a cardiac myocyte model that uses moment equations to represent heterogeneous junctional SR Ca2+.

    Science.gov (United States)

    Huertas, Marco A; Smith, Gregory D; Györke, Sándor

    2010-07-21

    Multiscale whole-cell models that accurately represent local control of Ca2+-induced Ca2+ release in cardiac myocytes can reproduce high-gain Ca2+ release that is graded with changes in membrane potential. Using a recently introduced formalism that represents heterogeneous local Ca2+ using moment equations, we present a model of cardiac myocyte Ca2+ cycling that exhibits alternating sarcoplasmic reticulum (SR) Ca2+ release when periodically stimulated by depolarizing voltage pulses. The model predicts that the distribution of junctional SR [Ca2+] across a large population of Ca2+ release units is distinct on alternating cycles. Load-release and release-uptake functions computed from this model give insight into how Ca2+ fluxes and stimulation frequency combine to determine the presence or absence of Ca2+ alternans. Our results show that the conditions for the onset of Ca2+ alternans cannot be explained solely by the steepness of the load-release function, but that changes in the release-uptake process also play an important role. We analyze the effect of the junctional SR refilling time constant on Ca2+ alternans and conclude that physiologically realistic models of defective Ca2+ cycling must represent the dynamics of heterogeneous junctional SR [Ca2+] without assuming rapid equilibration of junctional and network SR [Ca2+]. Copyright (c) 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  1. Phosphorylation and activation of nuclear Ca{sup 2+}/calmodulin-dependent protein kinase phosphatase (CaMKP-N/PPM1E) by Ca{sup 2+}/calmodulin-dependent protein kinase I (CaMKI)

    Energy Technology Data Exchange (ETDEWEB)

    Onouchi, Takashi [Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795 (Japan); Sueyoshi, Noriyuki, E-mail: sueyoshi@ag.kagawa-u.ac.jp [Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795 (Japan); Ishida, Atsuhiko [Laboratory of Molecular Brain Science, Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima 739-8521 (Japan); Kameshita, Isamu [Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795 (Japan)

    2012-06-15

    Highlights: Black-Right-Pointing-Pointer CaMKP-N/PPM1E underwent proteolytic processing and translocated to cytosol. Black-Right-Pointing-Pointer The proteolysis was effectively inhibited by the proteasome inhibitors. Black-Right-Pointing-Pointer Ser-480 of zebrafish CaMKP-N was phosphorylated by cytosolic CaMKI. Black-Right-Pointing-Pointer Phosphorylation-mimic mutants of CaMKP-N showed enhanced activity. Black-Right-Pointing-Pointer These results suggest that CaMKP-N is regulated by CaMKI. -- Abstract: Nuclear Ca{sup 2+}/calmodulin-dependent protein kinase phosphatase (CaMKP-N/PPM1E) is an enzyme that dephosphorylates and downregulates multifunctional Ca{sup 2+}/calmodulin-dependent protein kinases (CaMKs) as well as AMP-dependent protein kinase. In our previous study, we found that zebrafish CaMKP-N (zCaMKP-N) underwent proteolytic processing and translocated to cytosol in a proteasome inhibitor-sensitive manner. In the present study, we found that zCaMKP-N is regulated by phosphorylation at Ser-480. When zCaMKP-N was incubated with the activated CaMKI, time-dependent phosphorylation of the enzyme was observed. This phosphorylation was significantly reduced when Ser-480 was replaced by Ala, suggesting that CaMKI phosphorylates Ser-480 of zCaMKP-N. Phosphorylation-mimic mutants, S480D and S480E, showed higher phosphatase activities than those of wild type and S480A mutant in solution-based phosphatase assay using various substrates. Furthermore, autophosphorylation of CaMKII after ionomycin treatment was more severely attenuated in Neuro2a cells when CaMKII was cotransfected with the phosphorylation-mimic mutant of zCaMKP-N than with the wild-type or non-phosphorylatable zCaMKP-N. These results strongly suggest that phosphorylation of zCaMKP-N at Ser-480 by CaMKI activates CaMKP-N catalytic activity and thereby downregulates multifunctional CaMKs in the cytosol.

  2. Materials compatibility during the chlorination of molten CaCl2 . CaO salts

    International Nuclear Information System (INIS)

    Rense, C.E.C.; Fife, K.W.; Bowersox, D.F.; Ferran, M.D.

    1987-01-01

    As part of our effort to develop a semicontinuous PuO 2 reduction process, we are investigating promising materials for containing a 900 0 C molten CaCl 2 . CaO chlorination reaction. We want the material to contain this reaction and to be reusable. We tested candidate materials in a simulated salt (no plutonium) using anhydrous HCl as the chlorinating agent. Data are presented on the performance of 36 metals and alloys, 9 ceramics, and 3 coatings

  3. Anode process on platinum in CaCl2-CaO-based melt

    Directory of Open Access Journals (Sweden)

    K. V. Tatarenko

    2014-12-01

    Full Text Available Methods potentiostatic polarization and cyclic voltammetry obtained new data on the mechanism and kinetics of anodic processes on platinum in the molten CaCl2-KCl-CaO when 725–775 °C. Given thermodynamic values of potential difference probable total reactions in the range of the studied temperature. Using potentiostatic polarization and cyclic voltammetry obtained new data on the mechanism and kinetics of anodic process on platinum in the molten CaCl2-KCl-CaO when 725–775 °C thermodynamic assessment of the probability of occurrence total reactions during electrolysis melt on the basis of CaCl2-CaO using non-carbon anode. It is shown that at high current densities anodic process takes place mainly in the conditions of slow diffusion of electrically active particles to the anode, and at low densities (up to 10 mA/cm2 to their discharge to the atomic and lecular oxygen is preceded by a stage, which can be associated with adsorption of atoms of oxygen or with the formation of an oxide film on the surface of platinum. To detect the nature of this stage, further research is needed.

  4. Diagnostic significance of tumor markers CEA, CA50 and CA19-9 for colorectal cancer

    International Nuclear Information System (INIS)

    Chen Yumei; Huang Gang

    2005-01-01

    Objective: To investigate the expression and diagnostic significance of three serum tumor markers (CEA, CA50, CA19-9) in patients with colorectal cancer, with special emphasis on their combined assay. Methods: Serum CEA, CA19-9 levels (with chemiluminescence immunoassay) and CA50 levels (with immunoradiometric assay) were determined in 94 patients with colorectal cancer, 20 patients with benign colorectal disorders and 37 controls. Results: The expressions of the serum tumor markers were significantly higher in patients with colorectal cancer than those in patients with benign colorectal disorders and controls (P<0.05). There was no significant difference between the levels in the latter two groups. CEA assay had the highest sensitivity (57.4%) and specificity (85.9%). Combined assay of the three could enhance both the sensitivity (62.7%) and specificity (96.5%). The serum levels of the markers were significantly higher in patients with colonic cancer than those in patients with rectal cancer (P<0.05). The levels were positively correlated with the size of the growth and stage of the disease. Serum tumor marker levels were also significantly higher in patients with metastasis (regional/distant) than those in patients without metastasis (P<0.05). Conclusion: Determination of serum CEA, CA50 and CA19-9 levels had definite value for the diagnosis and assessment of the pathology as well as biologic behavior colorectal cancer. Combined assay of the three could enhance the diagnostic sensitivity and specificity. (authors)

  5. Neuronal Ca(2+) dyshomeostasis in Huntington disease.

    Science.gov (United States)

    Giacomello, Marta; Oliveros, Juan C; Naranjo, Jose R; Carafoli, Ernesto

    2013-01-01

    The expansion of the N-terminal poly-glutamine tract of the huntingtin (Htt) protein is responsible for Huntington disease (HD). A large number of studies have explored the neuronal phenotype of HD, but the molecular aethiology of the disease is still very poorly understood. This has hampered the development of an appropriate therapeutical strategy to at least alleviate its symptoms. In this short review, we have focused our attention on the alteration of a specific cellular mechanism common to all HD models, either genetic or induced by treatment with 3-NPA, i.e. the cellular dyshomeostasis of Ca(2+). We have highlighted the direct and indirect (i.e. transcriptionally mediated) effects of mutated Htt on the maintenance of the intracellular Ca(2+) balance, the correct modulation of which is fundamental to cell survival and the disturbance of which plays a key role in the death of the cell.

  6. Time-resolved fluoroimmunoassay of CA125

    International Nuclear Information System (INIS)

    Cai Gangming; Huang Biao; Zhu Liguo; Xiao Hualong; Tan Cheng; Tao Yonghui; Jin Jian

    2001-01-01

    A two-site time-resolved fluoroimmunoassay (TRFIA) of CA 125 based on the direct sandwich technique has been developed, with the equilibrium method. The monoclonal antibody (MoAb) against CA 125 was labelled with europium by the help of europium-chelate of diethylenetriaminepentaacetic acid (DTPA). The luminescent enhancement system was an enhancement solution that contained mainly of 2-naphthoyltrifluoroacetone. The intra- and inter- assay CV of the TRFIA were 4.5% and 4.0%, respectively, and the recovery rate was 96.7%, the sensitivity was 3.3 μg/mL. The cross-reacting rate with CEA was negligible, and that with AFP and β-HCG was 4.6% and 12.4%, respectively. Compared with the imported IRMA Kit, the correlation coefficient was 0.999

  7. „My tu nie mówimy, ino godumy i jesteśmy z tego dumni”. O tym, dlaczego gwary wielkopolskie przetrwały (na przykładzie języka mieszkańców wsi powiatu międzychodzkiego

    Directory of Open Access Journals (Sweden)

    Bartosz Cemborowski

    2015-12-01

    Full Text Available On why have the dialects of the Province of Greater Poland survived (an example, the language of Międzychodzki district inhabitants The aim of this article is to respond to the question: Why have the dialects of the Province of Greater Poland survived while the dialects from other parts of Poland are in decline? The analytical material relates to the statements of the users of this dialect who live in the western parts of Greater Poland (Międzychodzki district. It was collected during fieldwork in the villages of the Międzychodzki district between 2012 and 2015. The research methodology was based on autobiographical interviews, questionnaires and participant observations. Three important factors which contributed to the survival of the dialects of Greater Poland are: the history of this region, the identity of its people and the fashion for dialect use among the younger generation of Greater Poland’s inhabitants.   „My tu nie mówimy, ino godumy i jesteśmy z tego dumni”. O tym, dlaczego gwary wielkopolskie przetrwały (na przykładzie języka mieszkańców wsi powiatu międzychodzkiego Celem artykułu jest odpowiedź na pytanie, dlaczego gwary wielkopolskie przetrwały, podczas gdy w innych regionach Polski zauważalna jest tendencja do ich zaniku. Materiałem wykorzystanym w analizie są wypowiedzi mieszkańców wsi powiatu międzychodzkiego, którzy posługują się gwarą. Materiał został zebrany podczas badań terenowych w latach 2012–2015 za pomocą takich metod, jak wywiad autobiograficzny, ankiety i obserwacja uczestnicząca. Trzema istotnymi czynnikami, które wpłynęły na to, że gwary przetrwały w języku respondentów, są: historia, identyfikacja i narastająca wśród młodego pokolenia moda na mówienie gwarą.

  8. The Reliability of Highly Elevated CA 19-9 Levels

    Directory of Open Access Journals (Sweden)

    B. R. Osswald

    1993-01-01

    Full Text Available CA 19-9 is used as a tumour marker of the upper gastrointestinal tract. However, extremely elevated CA 19-9 levels are found also in patients with benign diseases. Cholestasis was present in 97.1 % of patients with high elevated CA 19-9, independent of their primary disease. 50% of patients with non-malignant diseases and increased CA 19-9 levels showed liver cirrhosis, cholecystitis, pancreatitis and/or hepatitis. In 8.8% no explanation was found for the extremely high CA 19-9 level. The results provide evidence of different factors influencing the CA 19-9 level.

  9. Direct and Inverse Scattering Problem Associated with the Elliptic Sinh-Gordon Equation

    Science.gov (United States)

    1989-11-14

    eye-opener. Not only do these soliton’s eigenvalues move, but theygrow without limit! Thus one can mathenatically treat SRS as a rure N-soliton solution...we can compute the second symplectic form I2=1 <SAIA0LA)-I V> f2 < = 8vAI~o2 (L 1l8V>. Now, according to (B3) of ref. 10, 8 8V .1 f 8 p(4) q’(4) - 8p(4...the continuous spectrum off the real axis to avoid the singularity at C = 0. Therefore the only difference between the computation of 0, and the new

  10. Soliton solutions of the generalized sinh-Gordon equation by the ...

    Indian Academy of Sciences (India)

    Pramana – Journal of Physics. Current Issue : Vol. 90, Issue 4 · Current Issue Volume 90 | Issue 4. April 2018. Home · Volumes & Issues · Special Issues · Forthcoming Articles · Search · Editorial Board · Information for Authors · Subscription ...

  11. Navier--Stokes relaxation to sinh--Poisson states at finite Reynolds numbers

    International Nuclear Information System (INIS)

    Montgomery, D.; Shan, X.; Matthaeus, W.H.

    1993-01-01

    A mathematical framework is proposed in which it seems possible to justify the computationally-observed relaxation of a two-dimensional Navier--Stokes fluid to a ''most probable,'' or maximum entropy, state. The relaxation occurs at large but finite Reynolds numbers, and involves substantial decay of higher-order ideal invariants such as enstrophy. A two-fluid formulation, involving interpenetrating positive and negative vorticity fluxes (continuous and square integrable) is developed, and is shown to be intimately related to the passive scalar decay problem. Increasing interpenetration of the two fluids corresponds to the decay of vorticity flux due to viscosity. It is demonstrated numerically that, in two dimensions, passive scalars decay rapidly, relative to mean-square vorticity (enstrophy). This observation provides a basis for assigning initial data to the two-fluid field variables

  12. Soliton solutions of the generalized sinh-Gordon equation by the ...

    Indian Academy of Sciences (India)

    2015-11-27

    Proceedings of the International Workshop/Conference on Computational Condensed Matter Physics and Materials Science (IWCCMP-2015). Posted on November 27, 2015. Guest Editors: Anurag Srivastava, C. S. Praveen, H. S. Tewari. © 2017 Indian Academy of Sciences, Bengaluru. Contact | Site index.

  13. Astrocyte Ca2+ signalling: an unexpected complexity

    OpenAIRE

    Volterra, Andrea; Liaudet, Nicolas; Savtchouk, Iaroslav

    2014-01-01

    Astrocyte Ca(2+) signalling has been proposed to link neuronal information in different spatial-temporal dimensions to achieve a higher level of brain integration. However, some discrepancies in the results of recent studies challenge this view and highlight key insufficiencies in our current understanding. In parallel, new experimental approaches that enable the study of astrocyte physiology at higher spatial-temporal resolution in intact brain preparations are beginning to reveal an unexpec...

  14. TU-CD-303-02: Beyond Radiation Induced Double Strand Breaks - a New Horizon for Radiation Therapy Research

    International Nuclear Information System (INIS)

    Chang, S.

    2015-01-01

    these advances in cancer biology research will give medical physicists a new perspective in daily clinical physics practice and in future radiation therapy technological development. Furthermore, academic medical physics should continue to be an integral part of the multidisciplinary cancer research community, harnessing our newly acquired understanding of radiation effects, and developing novel cost-effective treatment strategies to better combat cancer. Learning Objectives: Understand that localized radiation can lead to non-localized secondary effects such as radiation-induced immune response, bystander effect, and abscopal effect. Understand that the non-localized radiation effects may be harnessed to improve cancer treatment. Learn examples of physics participation in multidisciplinary research to advance cancer biology. Recognize the challenges and possibilities of physics applications in cancer research. Chang: NIH 5RC2CA148487-02 and 1U54CA151652-01 Graves: IDEA award (19IB-0106) from the California Breast Cancer Research Program (CBCRP), and by NIH P01 CA67166

  15. TU-CD-303-02: Beyond Radiation Induced Double Strand Breaks - a New Horizon for Radiation Therapy Research

    Energy Technology Data Exchange (ETDEWEB)

    Chang, S. [UNC School of Medicine (United States)

    2015-06-15

    these advances in cancer biology research will give medical physicists a new perspective in daily clinical physics practice and in future radiation therapy technological development. Furthermore, academic medical physics should continue to be an integral part of the multidisciplinary cancer research community, harnessing our newly acquired understanding of radiation effects, and developing novel cost-effective treatment strategies to better combat cancer. Learning Objectives: Understand that localized radiation can lead to non-localized secondary effects such as radiation-induced immune response, bystander effect, and abscopal effect. Understand that the non-localized radiation effects may be harnessed to improve cancer treatment. Learn examples of physics participation in multidisciplinary research to advance cancer biology. Recognize the challenges and possibilities of physics applications in cancer research. Chang: NIH 5RC2CA148487-02 and 1U54CA151652-01 Graves: IDEA award (19IB-0106) from the California Breast Cancer Research Program (CBCRP), and by NIH P01 CA67166.

  16. Mg,Ca-ATPase activity under irradiation

    International Nuclear Information System (INIS)

    Ladutin, V.V.; Orlova, V.V.; Lob, P.A.; Gerasiminko, I.V.; Mack, E.I.

    2003-01-01

    Full text: The influence of different doses irradiation at the Mg,Ca-ATPase activity at the rat brain has been investigated. The analyses were made at the apparatus of LKB and Carl-Ceis-Jena firm with help of reagents of Sigma and Boehringer firm. Rats decapitated after 1, 3, 6, 24 and 48 h after action of irradiation. Dose 0.206 C/kg. Erythrocytes. 1 and 3h after irradiation influence- decrease of Mg,Ca-ATPase activity to 86-87% relatively control level, 24 and 48 h - increase of activity to the control level. Dose 0.312 C/kg. Large hemispheres. 1h - decrease of ATPase activity to 90% relatively control, 3h - increase to control level, 24h - fall to 86%, after 48h small increase to 93% relatively control. Dose 9.287 C/kg. Large hemispheres. 1h - sharp fall of Mg, Ca-ATPase activity to 67 % relatively control, increase of activity to 96% after 3h and sharp fall of activity to 64% 6h after action of irradiation. Dose 9.287 C/kg. Cerebellum. 1h - sharp decrease of ATPase activity to 80%. After 3h -sharp increase to 160% relatively control level and sharp fall of ATPase activity to 47% relatively control after 6h. The mechanism of radiation pathology of active ion transport has been discussed

  17. TU-H-CAMPUS-TeP1-01: Variable-Beam Fractionation for SAbR

    International Nuclear Information System (INIS)

    Modiri, A; Sawant, A

    2016-01-01

    reducing dose to OARs. This work was partially supported through research funding from National Institutes of Health (R01CA169102) and Varian Medical Systems, Palo Alto, CA, USA.

  18. TU-H-CAMPUS-TeP1-01: Variable-Beam Fractionation for SAbR

    Energy Technology Data Exchange (ETDEWEB)

    Modiri, A; Sawant, A [University of Maryland School of Medicine, Baltimore, MD (United States)

    2016-06-15

    reducing dose to OARs. This work was partially supported through research funding from National Institutes of Health (R01CA169102) and Varian Medical Systems, Palo Alto, CA, USA.

  19. Saistība starp pusaudžu piederības izjūtu skolai un mācību sasniegumiem

    OpenAIRE

    Jēkabsone, Agnese

    2008-01-01

    Jēkabsone, A., bakalaura darbs „ Saistība starp pusaudžu piederības izjūtu skolai un mācību sasniegumiem”. Pētījuma teorētiskajā daļā autore apkopo un analizē Ā. Adlera, M. Balsona, E. Eriksona, J. Maļickas atziņas par piederības jēdzienu, tās uzbūvi, kā arī teorētiski pamato piederības izjūtas un mācību sasniegumu noteicošām pazīmes. Pētījuma empīriskajā daļā tiek veikta piederības izjūtas un mācību sasniegumu pazīmju empīriskā pārbaude. Datu ieguvei tika izmantota anketēšana, savukār...

  20. Proceso de educación intercultural en primera infancia: programa educa a tu hijo -Cuba- y programa hogares comunitarios de bienestar -HBC -Colombia

    Directory of Open Access Journals (Sweden)

    Cindy Tatiana Capera Espinel

    2017-09-01

    Full Text Available En el presente artículo se pretende hacer un análisis comparativo, desde una perspectiva intercultural, de dos programas educativos no convencionales, el programa cubano Educa a tu hijo y el programa colombiano Ho­gares Comunitarios de Bienestar —HCB—, dirigidos a la educación de la primera infancia. Se hace referencia a las características y estrategias en las que se evidencia el vínculo existente entre educación intercultural y la primera infancia, con el fin de fortalecer el desarrollo integral de niños y niñas. Esto surge como resultado de una experiencia de internacionalización en la que se desarrolló el curso de corta duración titulado «Didáctica de la inclusión social y la actividad física» durante dieciséis días en Cuba, ello como parte del proceso de formación de pedagogos infantiles de UNIMINUTO.

  1. Tu Amigo Pepe: Evaluation of a Multi-media Marketing Campaign that Targets Young Latino Immigrant MSM with HIV Testing Messages.

    Science.gov (United States)

    Solorio, Rosa; Norton-Shelpuk, Pamela; Forehand, Mark; Montaño, Daniel; Stern, Joshua; Aguirre, Joel; Martinez, Marcos

    2016-09-01

    Latino immigrant men who have sex with men (MSM) are at risk for HIV and delayed diagnosis in the United States. This paper describes the evaluation of a pilot of the Tu Amigo Pepe, a multimedia HIV testing campaign aimed at Latino MSM in Seattle, WA particularly targeting immigrants who may not identify as gay, ages 18-30 years old. The 16-week campaign included Spanish-language radio public service announcements (PSAs), a Web site, social media outreach, a reminder system using mobile technology, print materials and a toll-free hotline. In developing the PSAs, the Integrated Behavioral Model was used as a framework to reframe negative attitudes, beliefs and norms towards HIV testing with positive ones as well as to promote self-efficacy towards HIV testing. The campaign had a significant and immediate impact on attitudes, beliefs, norms and self-efficacy towards HIV testing as well as on actual behavior, with HIV testing rates increasing over time.

  2. La satisfacción de residentes con su colonia y el programa gubernamental Tu Casa en el estado de Zacatecas

    Directory of Open Access Journals (Sweden)

    Jorge Ibarra Salazar

    2016-01-01

    Full Text Available El grado de satisfacción residencial, considerado como un indicador de nivel de vida, tiene como uno de sus componentes la satisfacción con la colonia de los individuos. En este artículo estimamos una serie de modelos para analizar el grado de satisfacción con la colonia de una muestra de hogares en el estado de Zacatecas. Las estimaciones nos permiten determinar si el programa federal de vivienda Tu Casa en ese estado, operado de 2004 a 2009, tuvo alguna influencia en el grado de satisfacción con la colonia, de acuerdo con la percepción de los residentes entrevistados. Encontramos evidencia de que el referido programa causó un efecto significativo en el grado de satisfacción del grupo de familias beneficiarias. Además de ese resultado, la estimación por mínimos cuadrados ordinarios muestra que el acceso vial, la contaminación ambiental, la percepción del valor de la casa y el tiempo de vivir en la vivienda influyen de forma significativa en el grado de satisfacción con la colonia. En los modelos estimados por probit y logit encontramos evidencia de que la seguridad pública y la posesión del inmueble también influyen en tal satisfacción.

  3. CERN Library | Sandrine Saison-Marsollier, Corinne Pralavorio and Michel Spiro present "Si tu devais me dessiner l’Univers…" | 10 December

    CERN Multimedia

    CERN Library

    2015-01-01

    Sandrine Saison-Marsollier, Corinne Pralavorio and Michel Spiro present Si tu devais me dessiner l’Univers… Thursday, 10 December 2015 at 3.30 p.m. at the Library, Building 52-1-052 Tea and coffee will be served at 3 p.m. In 2014, CERN launched an art competition for local primary schools. Children were asked to come up with questions about the universe, matter and working as a researcher. The initiative was so successful that it has been turned into a book that teachers can explore with their pupils, discussing the questions and discovering the answers together. The book is laid out in double-page spreads, with the child’s illustration on one page and the question and its answer opposite. The answers take the form of a short text written by Michel Spiro, the competition’s scientific advisor, together with a cultural quotation whose purpose is to highlight the close association between science, literature and philosophy. The questions include: What was there...

  4. Tu e o de quem?" Tecendo os os com Bakhtin, Vigotski e Paulo Freire / Who do you think you are?" Commenting the wires with Bakhtin, Vigotski and Freire

    Directory of Open Access Journals (Sweden)

    Joselito Manoel de Jesus

    2010-08-01

    Full Text Available RESUMO: Este artigo analisa o enunciado "Tu é o de quem?", apontando para a responsabilidade que um filho da roça tem como partícipe do sustento da família e como essa realidade imediata constitui fios que enredam sua existência. Adota as contribuições de Bakhtin, Freire e Vigotski, evidenciando o processo de humanização como resultado das interações verbais e sociais que os indivíduos estabelecem em determinadas condições que caracterizam o espaço e o tempo em queestão inseridos e inserindo-se num processo ininterrupto. ABSTRACT:This article aims at analyzing the statement addressed to a farm child "Who do you think you are?" pointing to the fact that these children are responsible for their family income and as thisimmediate reality is intertwined by wires which build up their existence. This text is based on Bakhtin, Vygotsky and Freire, showing the process of humanization as a result of social and verbalinteractions which individuals establish under certain conditions in order to characterize the space and time in which they are inserted and inserting themselves into a never-ending process.

  5. Incidence and remission of parasomnias among adolescent children in the Tucson Children’s Assessment of Sleep Apnea (TuCASA Study

    Directory of Open Access Journals (Sweden)

    Furet O

    2011-06-01

    Full Text Available Background: Longitudinal assessments of parasomnias in the adolescent population are scarce. This analysis aims to identify the incidence and remission of parasomnias in the adolescent age group.Methods: The TuCASA study is a prospective cohort study that initially enrolled children between the ages of 6 and 11 years (Time 1 and subsequently re-studied them approximately 5 years later (Time 2. At both time points parents were asked to complete a comprehensive sleep habits questionnaire designed to assess the severity of sleep-related symptoms that included questions about enuresis (EN, sleep terrors (TR, sleep walking (SW and sleep talking (ST.Results: There were 350 children participating at Time 1 who were studied as adolescents at time 2. The mean interval between measurements was (4.6 years. The incidence of EN, TR, ST, and SW in these 10-18 year old children was 0.3%, 0.6%, 6.0% and 1.1% respectively. Remission rates were 70.8%, 100%, 64.8% and 50.0% respectively.Conclusions: The incidence rates of EN, TR, and SW were relatively low moving from childhood to adolescence while remission rates were high across all parasomnias.

  6. Daudzkanālu skaņas formātu izplatība un testēšanas rīki

    OpenAIRE

    Brēmers, Ronalds

    2014-01-01

    Mūsdienās mājas lietotāju vidū, stereo sistēmas tiek izmantotas visbiežāk, ko var secināt gan no statistikas par daudzkanālu audio formātu izplatību, kā arī paša pieredzes un tā, ka stereo sistēmas uzstādīšana un izmantošana prasa mazāk pūļu un resursus. Par spīti tam, filmu un mūzikas pilnai baudīšanai daudzi izvēlas tieši 5.1 vai 7.1 audio sistēmas konfigurāciju, kurām ir savi trūkumi, kā pareizas uzstādīšanas un konfigurēšanas problēmas, tā arī, galvenokārt, legāla satura iegūšanas grūtība...

  7. Glucagon releases Ca2+ from a FCCP-sensitive pool

    International Nuclear Information System (INIS)

    Kraus-Friedmann, N.

    1986-01-01

    The effects of physiological levels of glucagon on Ca 2+ efflux were examined in the perfused rat liver. Two methods were used to estimate Ca 2+ efflux: (1) prior labeling of the Ca 2+ pools with 45 Ca 2+ , and (2) measurement of perfusate Ca 2+ with atomic absorption. According to both methods, glucagon administration at the physiological level evoked Ca 2+ release. In order to identify the hormone-sensitive Ca 2+ pool, a method employed by several laboratories was used. In this method, mitochondrial Ca 2+ is released by FCCP, (carbonyl-cyanide 4 (trifluoro/methoxy) phenylhydrazone), a mitochondrial uncoupler. The effect of hormones on Ca 2+ release after such uncoupler administration is measured. A decreased release is taken as an indication that the pool is entirely or partially mitochondrial. FCCP released 90 +/- 29 nmoles Ca 2+ /gr wet liver. Glucagon (5 x 10 -9 M) released 107 +/- 45 nmoles Ca 2+ /gr wet liver before and 26 +/- 9 nmoles Ca 2+ /gr wet liver after FCCP. These data indicate that glucagon releases Ca 2+ mostly from the mitochondria

  8. CaMKII: linking heart failure and arrhythmias

    Science.gov (United States)

    Swaminathan, Paari Dominic; Purohit, Anil; Hund, Thomas J; Anderson, Mark E

    2013-01-01

    Understanding relationships between heart failure and arrhythmias, important causes of suffering and sudden death, remains an unmet goal for biomedical researchers and physicians. Evidence assembled over the last decade supports a view that activation of the multifunctional Ca2+ and calmodulin-dependent protein kinase II (CaMKII) favors myocardial dysfunction and cell membrane electrical instability. CaMKII activation follows increases in intracellular Ca2+ or oxidation, upstream signals with the capacity to transition CaMKII into a Ca2+ and calmodulin-independeant, constitutively active enzyme. Constitutively active CaMKII appears poised to participate in disease pathways by catalyzing the phosphorylation of classes of protein targets important for excitation-contraction coupling and cell survival, including ion channels and Ca2+ homeostatic proteins, and transcription factors that drive hypertrophic and inflammatory gene expression. This rich diversity of downstream targets helps to explain the potential for CaMKII to simultaneously affect mechanical and electrical properties of heart muscle cells. Proof of concept studies from a growing number of investigators show that CaMKII inhibition is beneficial for improving myocardial performance and reducing arrhythmias. Here we review the molecular physiology of CaMKII, discuss CaMKII actions at key cellular targets and results of animal models of myocardial hypertrophy, dysfunction and arrhythmias that suggest CaMKII inhibition may benefit myocardial function while reducing arrhythmias. PMID:22679140

  9. IP3 stimulates CA++ efflux from fusogenic carrot protoplasts

    International Nuclear Information System (INIS)

    Rincon, M.; Boss, W.F.

    1986-01-01

    Polyphosphoinositide breakdown plays an important role in signal transduction in animal cells (Berridge and Irvine, 1984, Nature, 312:315). Upon stimulation, phospholipase C hydrolyzes phosphatidylinositol 4,5-bisphosphate to inositol 1,4,5-trisphosphate (IP 3 ) and diacylglycerol both of which act as cellular second messengers. IP 3 mobilizes Ca ++ from internal stores, hence the cytosolic free Ca ++ concentration increases and those physiological activities regulated by Ca ++ are stimulated. To test if plant cells also responded to IP 3 , Ca ++ efflux studies were done with fusogenic carrot protoplasts released in EGTA. The protoplasts were preloaded with 45 Ca ++ placed in a Ca ++ -free medium, and efflux determined as 45 Ca ++ loss from the protoplasts. IP 3 (10-20μM) caused enhanced 45 Ca ++ efflux and the response was sustained for at least 15 min. In plants, as in animals, the observed IP 3 -enhanced 45 Ca ++ efflux suggested that IP 3 released Ca ++ from internal stores, and the increased free cytosolic Ca ++ activated Ca ++ pumping mechanisms which restored the Ca ++ concentration in the cytosol to the normal level

  10. Determination of the calcium species in coal chars by Ca K-edge XANES analysis

    Science.gov (United States)

    Liu, Li-Juan; Liu, Hui-Jun; Cui, Ming-Qi; Hu, Yong-Feng; Zheng, Lei; Zhao, Yi-Dong; Ma, Chen-Yan; Xi, Shi-Bo; Yang, Dong-Liang; Guo, Zhi-Ying; Wang, Jie

    2013-02-01

    Ca-based additives have been widely used as a sulfur adsorbent during coal pyrolysis and gasification. The Ca speciation and evolution during the pyrolysis of coal with Ca additives have attracted great attention. In this paper, Ca species in the coal chars prepared from the pyrolysis of Ca(OH)2 or CaCO3-added coals are studied by using Ca K-edge X-ray absorption near-edge structural spectroscopy. The results demonstrate that Ca(OH)2, CaSO4, CaS and CaO coexist in the Ca(OH)2-added chars, while Ca(OH)2 and CaSO4 are the main species in the Ca(OH)2-added chars. Besides, a carboxyl-bound Ca is also formed during both the pyrolysis for the Ca(OH)2-added and the CaCO3-added coals. A detailed discussion about the Ca speciation is given.

  11. Selective reinnervation of hippocampal area CA1 and the fascia dentata after destruction of CA3-CA4 afferents with kainic acid.

    Science.gov (United States)

    Nadler, J V; Perry, B W; Cotman, C W

    1980-01-20

    Intraventricular injections of kainic acid were used to destroy the hippocampal CA3-CA4 cells, thus denervating the inner third of the molecular layer of the fascia dentata and stratum radiatum and stratum oriens of area CA1. The responses of intact afferents to such lesions were then examined histologically. The hippocampal mossy fibers densely reinnervated the inner portion of the dentate molecular layer after bilateral destruction of CA4 neurons and to a lesser extent after unilateral destruction. Septohippocampal fibers replaced CA4-derived fibers in the dentate molecular layer only after particularly extensive bilateral CA4 lesions. Medial perforant path fibers showed no anatomical response to any of these lesions. Neither septohippocampal, temporoammonic nor mossy fibers proliferated in or grew into the denervated laminae of area CA1. These results show a preferential ordering in the reinnervation of dentate granule cells which is not readily explained by proximity to the degenerating fibers and also that removal of CA3-CA4-derived innervation more readily elicits translaminar growth in the fascia dentata than in area CA1. These results may be relevant to clinical situations in which neurons of the hippocampal end-blade are lost.

  12. Characterization of Bacillus subtilis YfkE (ChaA): a calcium-specific Ca2+/H+ antiporter of the CaCA family.

    Science.gov (United States)

    Fujisawa, Makoto; Wada, Yuko; Tsuchiya, Takahiro; Ito, Masahiro

    2009-08-01

    YfkE, a protein from Bacillus subtilis, exhibits homology to the Ca(2+):Cation Antiporter (CaCA) Family. In a fluorescence-based assay of everted membrane vesicles prepared from Na(+)(Ca(2+))/H(+) antiporter-defective mutant Escherichia coli KNabc, YfkE exhibited robust Ca(2+)/H(+) antiport activity, with a K (m) for Ca(2+) estimated at 12.5 muM at pH 8.5 and 113 muM at pH 7.5. Neither Na(+) nor K(+) served as a substrate. Mg(2+) also did not serve as a substrate, but inhibited the Ca(2+)/H(+) antiporter activity. The Ca(2+) transport capability of YfkE was also observed directly by transport assays in everted membrane vesicles using radiolabeled (45)Ca(2+). Transcriptional analysis from the putative yfkED operon using beta-garactosidase activity as a reporter revealed that both of the yfkE and yfkD genes are regulated by forespore-specific sigma factor, SigG, and the general stress response regulator, SigB. These results suggest that YfkE may be needed for Ca(2+) signaling in the sporulation or germination process in B. subtilis. ChaA is proposed as the designation for YfkE of B. subtilis.

  13. Is tissue CA125 expression in epithelial ovarian adenocarcinoma heterogenic?

    DEFF Research Database (Denmark)

    Sparholt, Morten H; Høgdall, Claus K; Nedergaard, Lotte

    2013-01-01

    To evaluate if heterogeneity of tissue cancer antigen 125 (CA125) expression is present in epithelial serous adenocarcinomas. Furthermore, to investigate whether there is a correlation between levels of CA125 tissue expression, serum level of CA125, stage, and grade. A total of 10 patients...... diagnosed with serous ovarian adenocarcinomas were included. Preoperative blood samples were collected to determine serum CA125 levels. Tumor tissue from primary surgery was collected and processed for immunohistochemical analyses. CA125 was expressed in varying degrees in tumor tissues from all patients....... Mean tissue CA125 expression for each patient ranged from 36% to 98%. Intrapatient variations in tissue expression ranged from 10% to 90% point. No significant correlations between levels of CA125 tissue expression, serum level of CA125, stage, and grade were found. We found that the tissue expression...

  14. Store-operated Ca2+ entry is not required for fertilization-induced Ca2+ signaling in mouse eggs.

    Science.gov (United States)

    Bernhardt, Miranda L; Padilla-Banks, Elizabeth; Stein, Paula; Zhang, Yingpei; Williams, Carmen J

    2017-07-01

    Repetitive oscillations in cytoplasmic Ca 2+ due to periodic Ca 2+ release from the endoplasmic reticulum (ER) drive mammalian embryo development following fertilization. Influx of extracellular Ca 2+ to support the refilling of ER stores is required for sustained Ca 2+ oscillations, but the mechanisms underlying this Ca 2+ influx are controversial. Although store-operated Ca 2+ entry (SOCE) is an appealing candidate mechanism, several groups have arrived at contradictory conclusions regarding the importance of SOCE in oocytes and eggs. To definitively address this question, Ca 2+ influx was assessed in oocytes and eggs lacking the major components of SOCE, the ER Ca 2+ sensor STIM proteins, and the plasma membrane Ca 2+ channel ORAI1. We generated oocyte-specific conditional knockout (cKO) mice for Stim1 and Stim2, and also generated Stim1/2 double cKO mice. Females lacking one or both STIM proteins were fertile and their ovulated eggs displayed normal patterns of Ca 2+ oscillations following fertilization. In addition, no impairment was observed in ER Ca 2+ stores or Ca 2+ influx following store depletion. Similar studies were performed on eggs from mice globally lacking ORAI1; no abnormalities were observed. Furthermore, spontaneous Ca 2+ influx was normal in oocytes from Stim1/2 cKO and ORAI1-null mice. Finally, we tested if TRPM7-like channels could support spontaneous Ca 2+ influx, and found that it was largely prevented by NS8593, a TRPM7-specific inhibitor. Fertilization-induced Ca 2+ oscillations were also impaired by NS8593. Combined, these data robustly show that SOCE is not required to support appropriate Ca 2+ signaling in mouse oocytes and eggs, and that TRPM7-like channels may contribute to Ca 2+ influx that was previously attributed to SOCE. Published by Elsevier Ltd.

  15. MicroRNA-145 suppresses ROS-induced Ca{sup 2+} overload of cardiomyocytes by targeting CaMKIIδ

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Min-Ji [Cardiovascular Research Institute, Yonsei University College of Medicine, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Jang, Jin-Kyung [College of Pharmacy, Sookmyung Women’s University, 52 HyoChangWon-Gil, Yongsan-ku, Seoul 140-742 (Korea, Republic of); Ham, Onju; Song, Byeong-Wook; Lee, Se-Yeon [Cardiovascular Research Institute, Yonsei University College of Medicine, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Lee, Chang Yeon; Park, Jun-Hee [Department of Integrated Omics for Biomedical Sciences, Graduate School, Yonsei University, 50 Yonsei-ro, Seodamun-gu, Seoul 120-759 (Korea, Republic of); Lee, Jiyun; Seo, Hyang-Hee [Cardiovascular Research Institute, Yonsei University College of Medicine, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Choi, Eunhyun [Severance Integrative Research Institute for Cerebral and Cardiovascular Disease, Yonsei University Health System, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Jeon, Woo-min [Department of Animal Resource, Sahmyook University, Seoul 139-742 (Korea, Republic of); Hwang, Hye Jin [Cardiovascular Research Institute, Yonsei University College of Medicine, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Shin, Hyun-Taek [College of Pharmacy, Sookmyung Women’s University, 52 HyoChangWon-Gil, Yongsan-ku, Seoul 140-742 (Korea, Republic of); and others

    2013-06-14

    Highlights: •CaMKIIδ mediates H{sub 2}O{sub 2}-induced Ca{sup 2+} overload in cardiomyocytes. •miR-145 can inhibit Ca{sup 2+} overload. •A luciferase assay confirms that miR-145 functions as a CaMKIIδ-targeting miRNA. •Overexpression of miR-145 regulates CaMKIIδ-related genes and ameliorates apoptosis. -- Abstract: A change in intracellular free calcium (Ca{sup 2+}) is a common signaling mechanism of reperfusion-induced cardiomyocyte death. Calcium/calmodulin dependent protein kinase II (CaMKII) is a critical regulator of Ca{sup 2+} signaling and mediates signaling pathways responsible for functions in the heart including hypertrophy, apoptosis, arrhythmia, and heart disease. MicroRNAs (miRNA) are involved in the regulation of cell response, including survival, proliferation, apoptosis, and development. However, the roles of miRNAs in Ca{sup 2+}-mediated apoptosis of cardiomyocytes are uncertain. Here, we determined the potential role of miRNA in the regulation of CaMKII dependent apoptosis and explored its underlying mechanism. To determine the potential roles of miRNAs in H{sub 2}O{sub 2}-mediated Ca{sup 2+} overload, we selected and tested 6 putative miRNAs that targeted CaMKIIδ, and showed that miR-145 represses CaMKIIδ protein expression and Ca{sup 2+} overload. We confirmed CaMKIIδ as a direct downstream target of miR-145. Furthermore, miR-145 regulates Ca{sup 2+}-related signals and ameliorates apoptosis. This study demonstrates that miR-145 regulates reactive oxygen species (ROS)-induced Ca{sup 2+} overload in cardiomyocytes. Thus, miR-145 affects ROS-mediated gene regulation and cellular injury responses.

  16. Caffeine- and ryanodine-sensitive Ca(2+)-induced Ca2+ release from the endoplasmic reticulum in honeybee photoreceptors.

    Science.gov (United States)

    Walz, B; Baumann, O; Zimmermann, B; Ciriacy-Wantrup, E V

    1995-04-01

    Light stimulation of invertebrate microvillar photoreceptors causes a large rapid elevation in Cai, shown previously to modulate the adaptational state of the cells. Cai rises, at least in part, as a result of Ins(1,4,5)P3-induced Ca2+ release from the submicrovillar endoplasmic reticulum (ER). Here, we provide evidence for Ca(2+)-induced Ca2+ release (CICR) in an insect photoreceptor. In situ microphotometric measurements of Ca2+ fluxes across the ER membrane in permeabilized slices of drone bee retina show that (a) caffeine induces Ca2+ release from the ER; (b) caffeine and Ins(1,4,5)P3 open distinct Ca2+ release pathways because only caffeine-induced Ca2+ release is ryanodine sensitive and heparin insensitive, and because caffeine and Ins(1,4,5)P3 have additive effects on the rate of Ca2+ release; (c) Ca2+ itself stimulates release of Ca2+ via a ryanodine-sensitive pathway; and (d) cADPR is ineffective in releasing Ca2+. Microfluorometric intracellular Ca2+ measurements with fluo-3 indicate that caffeine induces a persistent elevation in Cai. Electrophysiological recordings demonstrate that caffeine mimics all aspects of Ca(2+)-mediated facilitation and adaptation in drone photoreceptors. We conclude that the ER in drone photoreceptors contains, in addition to the Ins(1,4,5)P3-sensitive release pathway, a CICR pathway that meets key pharmacological criteria for a ryanodine receptor. Coexpression of both release mechanisms could be required for the production of rapid light-induced Ca2+ elevations, because Ca2+ amplifies its own release through both pathways by a positive feedback. CICR may also mediate the spatial spread of Ca2+ release from the submicrovillar ER toward more remote ER subregions, thereby activating Ca(2+)-sensitive cell processes that are not directly involved in phototransduction.

  17. Modeling CaMKII-mediated regulation of L-type Ca2+ channels and ryanodine receptors in the heart

    Directory of Open Access Journals (Sweden)

    Joseph L Greenstein

    2014-04-01

    Full Text Available Excitation-contraction coupling (ECC in the cardiac myocyte is mediated by a number of highly integrated mechanisms of intracellular Ca2+ transport. Voltage- and Ca2+-dependent L-type Ca2+ channels (LCCs allow for Ca2+ entry into the myocyte, which then binds to nearby ryanodine receptors (RyRs and triggers Ca2+ release from the sarcoplasmic reticulum in a process known as Ca2+-induced Ca2+ release. The highly coordinated Ca2+-mediated interaction between LCCs and RyRs is further regulated by the cardiac isoform of the Ca2+/calmodulin-dependent protein kinase (CaMKII. Because CaMKII targets and modulates the function of many ECC proteins, elucidation of its role in ECC and integrative cellular function is challenging and much insight has been gained through the use of detailed computational models. Multiscale models that can both reconstruct the detailed nature of local signaling events within the cardiac dyad and predict their functional consequences at the level of the whole cell have played an important role in advancing our understanding of CaMKII function in ECC. Here, we review experimentally based models of CaMKII function with a focus on LCC and RyR regulation, and the mechanistic insights that have been gained through their application.

  18. New anticancer drug candidates sulfonamides as selective hCA IX or hCA XII inhibitors.

    Science.gov (United States)

    Gul, Halise Inci; Yamali, Cem; Sakagami, Hiroshi; Angeli, Andrea; Leitans, Janis; Kazaks, Andris; Tars, Kaspars; Ozgun, Dilan Ozmen; Supuran, Claudiu T

    2018-04-01

    In this study, new 4-[3-(aryl)-5-substitutedphenyl-4,5-dihydro-1H-pyrazole-1-yl]benzensulfonamides (19-36) were synthesized and evaluated their cytotoxic/anticancer and CA inhibitory effects. According to results obtained, the compounds 34 (4-[5-(2,3,4-trimethoxyphenyl)-3-(thiophen-2-yl)-4,5-dihydro-1H-pyrazole-1-yl] benzensulfonamide, Potency-Selectivity Expression (PSE) = 141) and 36 (4-[5-(3,4,5-trimethoxyphenyl)-3-(thiophen-2-yl)-4,5-dihydro-1H-pyrazole-1-yl]benzensulfonamide, PSE = 54.5) were found the leader anticancer compounds with the highest PSE values. In CA inhibitory studies, the compounds 36 and 24 (4-[5-(3,4,5-trimethoxyphenyl)-3-(4-fluorophenyl)-4,5-dihydro-1H-pyrazole-1-yl]benzensulfonamide) were found the leader CA inhibitors depending on selectivity ratios. The compound 36 was a selective inhibitor of hCA XII isoenzyme (hCA I/hCA XII = 1250 and hCA II/hCA XII = 224) while the compound 24 was a selective inhibitor of hCA IX isoenzyme (hCA I/hCA IX = 161 and hCA II/hCA IX = 177). The compounds 24, 34, and 36 can be considered to develop new anticancer drug candidates. Copyright © 2018 Elsevier Inc. All rights reserved.

  19. Sensing mechanisms involved in Ca2+ and Mg2+ homeostasis

    NARCIS (Netherlands)

    Ferre, S.; Hoenderop, J.G.J.; Bindels, R.J.M.

    2012-01-01

    Calcium (Ca(2+)) and magnesium (Mg(2+)) ions are involved in many vital physiological functions. In the human body, Ca(2+) and Mg(2+) homeostatic systems rely on three components: (i) tissues (re)absorbing or storing Ca(2+) and Mg(2+), mainly kidney, intestine, and bone; (ii) hormones that modulate

  20. Electronic structure of Ca, Sr, and Ba under pressure.

    Science.gov (United States)

    Animalu, A. O. E.; Heine, V.; Vasvari, B.

    1967-01-01

    Electronic band structure calculations phase of Ca, Sr and Ba over wide range of atomic volumes under pressure electronic band structure calculations for fcc phase of Ca, Sr and Ba over wide range of atomic volumes under pressure electronic band structure calculations for fcc phase of Ca, Sr and Ba over wide range of atomic volumes under pressure

  1. Enhanced sarcoplasmic reticulum Ca(2+) release following intermittent sprint training

    DEFF Research Database (Denmark)

    Ørtenblad, Niels; Lunde, Per; Levin, Kasper

    2000-01-01

    -977) arbitrary units Ca(2+). g protein(-1). min(-1) (after). The relative SR density of functional ryanodine receptors (RyR) remained unchanged after training; there was, however, a 48% (P RyR. No significant differences in Ca(2+) uptake rate and Ca(2+)-ATPase capacity were...

  2. Registration of CA0469C025C chickpea germplasm

    Science.gov (United States)

    Chickpea (Cicer arientinum L.) germplasm CA0469C025C (Reg. No. XXX; PI XXX), was released by the USDA-ARS in 2010. CA0469C025C was released based on its improved yield and reaction to Ascochyta blight relative to the popular commercial cultivars ‘Dwelley’, ‘Sierra’, and ‘Sawyer’. CA0490C025C is deri...

  3. Integrated Mechanisms of CaMKII-dependent ventricular remodeling

    Directory of Open Access Journals (Sweden)

    Michael M Kreusser

    2014-03-01

    Full Text Available CaMKII has been shown to be activated during different cardiac pathological processes, and CaMKII-dependent mechanisms contribute to pathological cardiac remodeling, cardiac arrhythmias and contractile dysfunction during heart failure. Activation of CaMKII during cardiac stress results in a broad number of biological effects such as, on the one hand, acute effects due to phosphorylation of distinct cellular proteins as ion channels and calcium handling proteins and, on the other hand, integrative mechanisms by changing gene expression. This review focuses on transcriptional and epigenetic effects of CaMKII activation during chronic cardiac remodeling. Multiple mechanisms have been described how CaMKII mediates changes in cardiac gene expression. CaMKII has been shown to directly phosphorylate components of the cardiac gene regulation machinery. CaMKII phosphorylates several transcription factors such as CREB that induces the activation of specific gene programs. CaMKII activates transcriptional regulators also indirectly by phosphorylating histone deacetylases, especially HDAC4, which in turn inhibits transcription factors that drive cardiac hypertrophy, fibrosis and dysfunction. Recent studies demonstrate that CaMKII also phosphorylate directly histones, which may contribute to changes in gene expression. These findings of CaMKII-dependent gene regulation during cardiac remodeling processes suggest novel strategies for CaMKII-dependent transcriptional or epigenetic therapies to control cardiac gene expression and function. Manipulation of CaMKII-dependent signaling pathways in the settings of pathological cardiac growth, remodeling, and heart failure represents an auspicious therapeutic approach.

  4. High-capacity Ca2+ binding of human skeletal calsequestrin.

    Science.gov (United States)

    Sanchez, Emiliano J; Lewis, Kevin M; Danna, Benjamin R; Kang, Chulhee

    2012-03-30

    Calsequestrin, the major calcium storage protein in both cardiac and skeletal muscle, binds large amounts of Ca(2+) in the sarcoplasmic reticulum and releases them during muscle contraction. For the first time, the crystal structures of Ca(2+) complexes for both human (hCASQ1) and rabbit (rCASQ1) skeletal calsequestrin were determined, clearly defining their Ca(2+) sequestration capabilities through resolution of high- and low-affinity Ca(2+)-binding sites. rCASQ1 crystallized in low CaCl(2) buffer reveals three high-affinity Ca(2+) sites with trigonal bipyramidal, octahedral, and pentagonal bipyramidal coordination geometries, along with three low-affinity Ca(2+) sites. hCASQ1 crystallized in high CaCl(2) shows 15 Ca(2+) ions, including the six Ca(2+) ions in rCASQ1. Most of the low-affinity sites, some of which are μ-carboxylate-bridged, are established by the rotation of dimer interfaces, indicating cooperative Ca(2+) binding that is consistent with our atomic absorption spectroscopic data. On the basis of these findings, we propose a mechanism for the observed in vitro and in vivo dynamic high-capacity and low-affinity Ca(2+)-binding activity of calsequestrin.

  5. High-capacity Ca2+ Binding of Human Skeletal Calsequestrin*

    Science.gov (United States)

    Sanchez, Emiliano J.; Lewis, Kevin M.; Danna, Benjamin R.; Kang, ChulHee

    2012-01-01

    Calsequestrin, the major calcium storage protein in both cardiac and skeletal muscle, binds large amounts of Ca2+ in the sarcoplasmic reticulum and releases them during muscle contraction. For the first time, the crystal structures of Ca2+ complexes for both human (hCASQ1) and rabbit (rCASQ1) skeletal calsequestrin were determined, clearly defining their Ca2+ sequestration capabilities through resolution of high- and low-affinity Ca2+-binding sites. rCASQ1 crystallized in low CaCl2 buffer reveals three high-affinity Ca2+ sites with trigonal bipyramidal, octahedral, and pentagonal bipyramidal coordination geometries, along with three low-affinity Ca2+ sites. hCASQ1 crystallized in high CaCl2 shows 15 Ca2+ ions, including the six Ca2+ ions in rCASQ1. Most of the low-affinity sites, some of which are μ-carboxylate-bridged, are established by the rotation of dimer interfaces, indicating cooperative Ca2+ binding that is consistent with our atomic absorption spectroscopic data. On the basis of these findings, we propose a mechanism for the observed in vitro and in vivo dynamic high-capacity and low-affinity Ca2+-binding activity of calsequestrin. PMID:22337878

  6. Significance of the tumor markers CA 125 and CA 15-3 in postoperative diagnosis of ovarian and breast cancer

    International Nuclear Information System (INIS)

    Johannsen, B.; Bartel, U.; Elling, D.

    1989-01-01

    In 271 patients with ovarian carcinoma, benign ovarian tumors, breast cancer, and two control groups, serum levels of CA 125, CA 15-3, CEA and, partly, CA 19-9 were determined immunoradiometrically. According to the results of the determination of CA 125 in the follow-up of ovarian carcinoma, CA 125 represents a useful marker for early detection of recurrences, especially in cases of diffuse carcinoma dissemination. In incomplete tumor debulking, postoperative CA 125 serum levels did not prove to be helpful except that a positive level renders invasive diagnostic investigation no longer necessary. Postoperative follow-up in breast cancer early reveals distant metastases, with very high levels in patients with bone metastases. By simultaneous measurement of CA 15-3 and CEA the sensitivity could be increased from 86% (CA 15-3 only) to 93%. (author)

  7. TU-FG-201-07: Development of SRS Conical Collimator Collision Prediction Software for Radiation Treatment Safety

    International Nuclear Information System (INIS)

    Gutti, V; Morrow, A; Kim, S; Patel, M

    2016-01-01

    Purpose: Stereotactic radiosurgery (SRS) treatments using conical collimators can potentially result in gantry collision with treatment table due to limited collision-clear spaces. An in-house software was developed to help the SRS treatment planner mitigate potential SRS conical collimator (Varian Medical System, Palo Alto, CA) collisions with the treatment table. This software was designed to remove treatment re-planning secondary to unexpected collisions. Methods: A BrainLAB SRS ICT Frameless Extension used for SRS treatments in our clinic was mathematically modelled using surface points registered to the 3D co-ordinate space of the couch extension. The surface points are transformed based on the treatment isocenter point and potential collisions are determined in 3D space for couch and gantry angle combinations. The distance between the SRS conical collimators and LINAC isocenter is known. The collision detection model was programmed in MATLAB (Mathwork, Natick, MA) to display graphical plots of the calculations, and the plotted data is used to avoid the gantry and couch angle combinations that would likely result in a collision. We have utilized the cone collision tool for 23 SRS cone treatment plans (8 retrospective and 15 prospective for 10 patients). Results: Twenty one plans strongly agreed with the software tool prediction for collision. However, in two plans, a collision was observed with a 0.5 cm margin when the software predicted no collision. Therefore, additional margins were added to the clearance criteria in the program to achieve a lower risk of actual collisions. Conclusion: Our in-house developed collision check software successfully avoided SRS cone re-planning by 91.3% due to a reduction in cone collisions with the treatment table. Future developments to our software will include a CT image data set based collision prediction model as well as a beam angle optimization tool to avoid normal critical tissues as well as previously treated lesions.

  8. TU-G-210-03: Acoustic Simulations in Transcranial MRgFUS: Treatment Prediction and Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Vyas, U. [Stanford University (United States)

    2015-06-15

    Modeling can play a vital role in predicting, optimizing and analyzing the results of therapeutic ultrasound treatments. Simulating the propagating acoustic beam in various targeted regions of the body allows for the prediction of the resulting power deposition and temperature profiles. In this session we will apply various modeling approaches to breast, abdominal organ and brain treatments. Of particular interest is the effectiveness of procedures for correcting for phase aberrations caused by intervening irregular tissues, such as the skull in transcranial applications or inhomogeneous breast tissues. Also described are methods to compensate for motion in targeted abdominal organs such as the liver or kidney. Douglas Christensen – Modeling for Breast and Brain HIFU Treatment Planning Tobias Preusser – TRANS-FUSIMO - An Integrative Approach to Model-Based Treatment Planning of Liver FUS Urvi Vyas – Acoustic Simulations in Transcranial MRgFUS: Treatment Prediction and Analysis Learning Objectives: Understand the role of acoustic beam modeling for predicting the effectiveness of therapeutic ultrasound treatments. Apply acoustic modeling to specific breast, liver, kidney and transcranial anatomies. Determine how to obtain appropriate acoustic modeling parameters from clinical images. Understand the separate role of absorption and scattering in energy delivery to tissues. See how organ motion can be compensated for in ultrasound therapies. Compare simulated data with clinical temperature measurements in transcranial applications. Supported by NIH R01 HL172787 and R01 EB013433 (DC); EU Seventh Framework Programme (FP7/2007-2013) under 270186 (FUSIMO) and 611889 (TRANS-FUSIMO)(TP); and P01 CA159992, GE, FUSF and InSightec (UV)

  9. Chemical purification of CaCO{sub 3} and CaWO{sub 4} powders used for CaWO{sub 4} crystal production for the CRESST experiment

    Energy Technology Data Exchange (ETDEWEB)

    Trinh Thi, H.H.; Defay, X.; Erb, A.; Hampf, R.; Lanfranchi, J.C.; Langenkaemper, A.; Morgalyuk, V.; Muenster, A.; Mondragon, E.; Oppenheimer, C.; Potzel, W.; Schoenert, S.; Steiger, H.; Ulrich, A.; Wawoczny, S.; Willers, M.; Zoeller, A. [Physik-Department and Excellence Cluster Universe, Technische Universitaet Muenchen, Garching (Germany)

    2016-07-01

    CRESST (Cryogenic Rare Event Search with Superconducting Thermometers) uses CaWO{sub 4} single crystals as targets for the direct search for dark matter particles. Since several years these CaWO{sub 4} crystals are grown at the Technische Universitaet Muenchen. Thereby, commercially available CaCO{sub 3} and WO{sub 3} powders are used for the synthesis of CaWO{sub 4} powder. For the experiment low intrinsic contaminations of the crystals play a crucial role. In order to improve the radiopurity of the crystals it is necessary to reduce potential sources for radioactive backgrounds such as U and Th. In this poster we will present our studies of the chemical purification of the CaCO{sub 3} and CaWO{sub 4} powders.

  10. Mechanics of Old Faithful Geyser, Calistoga, CA

    Science.gov (United States)

    Rudolph, M.L.; Manga, M.; Hurwitz, Shaul; Johnston, Malcolm J.; Karlstrom, L.; Wang, Chun-Yong

    2012-01-01

    In order to probe the subsurface dynamics associated with geyser eruptions, we measured ground deformation at Old Faithful Geyser of Calistoga, CA. We present a physical model in which recharge during the period preceding an eruption is driven by pressure differences relative to the aquifer supplying the geyser. The model predicts that pressure and ground deformation are characterized by an exponential function of time, consistent with our observations. The geyser's conduit is connected to a reservoir at a depth of at least 42 m, and pressure changes in the reservoir can produce the observed ground deformations through either a poroelastic or elastic mechanical model.

  11. Tumor markers CA19-9, CA242 and CEA in the diagnosis of pancreatic cancer: a meta-analysis.

    Science.gov (United States)

    Zhang, Yimin; Yang, Jun; Li, Hongjuan; Wu, Yihua; Zhang, Honghe; Chen, Wenhu

    2015-01-01

    Pancreatic cancer has the worst prognosis and early detection is crucial for improving patient prognosis. Therefore, we performed a meta-analysis to evaluate and compare the sensitivity and specificity of single test of CA19-9, CA242, and CEA, as well as combination test in pancreatic cancer detection. We searched PubMed, Embase, Medline, and Wanfang databases for studies that evaluated the diagnostic validity of CA19-9, CA242, and CEA between January 1990 and September 2014. Data were analyzed by Meta-Disc and STATA software. A total of 21 studies including 3497 participants, which fulfilled the inclusion criteria were considered for analysis. The pooled sensitivities for CA19-9, CA242, and CEA were 75.4 (95% CI: 73.4-77.4), 67.8 (95% CI: 65.5-70), and 39.5 (95% CI: 37.3-41.7), respectively. The pooled specificities of CA19-9, CA242, and CEA were 77.6 (95% CI: 75.4-79.7), 83 (95% CI: 81-85), and 81.3 (95% CI: 79.3-83.2), respectively. Parallel combination of CA19-9+CA242 has a higher sensitivity (89, 95% CI: 80-95) without impairing the specificity (75, 95% CI: 67-82). Our meta-analysis showed that CA242 and CA19-9 have better performance in the diagnosis of pancreatic cancer than CEA. Furthermore, parallel combination test of CA19-9+CA242 could be of better diagnostic value than individual CA242 or CA19-9 test.

  12. Caffeine- and ryanodine-sensitive Ca(2+)-induced Ca2+ release from the endoplasmic reticulum in honeybee photoreceptors

    OpenAIRE

    1995-01-01

    Light stimulation of invertebrate microvillar photoreceptors causes a large rapid elevation in Cai, shown previously to modulate the adaptational state of the cells. Cai rises, at least in part, as a result of Ins(1,4,5)P3-induced Ca2+ release from the submicrovillar endoplasmic reticulum (ER). Here, we provide evidence for Ca(2+)- induced Ca2+ release (CICR) in an insect photoreceptor. In situ microphotometric measurements of Ca2+ fluxes across the ER membrane in permeabilized slices of dron...

  13. The other side of cardiac Ca2+ signaling: transcriptional control

    Directory of Open Access Journals (Sweden)

    Alejandro eDomínguez-Rodríquez

    2012-11-01

    Full Text Available Ca2+ is probably the most versatile signal transduction element used by all cell types. In the heart, it is essential to activate cellular contraction in each heartbeat. Nevertheless Ca2+ is not only a key element in excitation-contraction coupling (EC coupling, but it is also a pivotal second messenger in cardiac signal transduction, being able to control processes such as excitability, metabolism, and transcriptional regulation. Regarding the latter, Ca2+ activates Ca2+-dependent transcription factors by a process called excitation-transcription coupling (ET coupling. ET coupling is an integrated process by which the common signaling pathways that regulate EC coupling activate transcription factors. Although ET coupling has been extensively studied in neurons and other cell types, less is known in cardiac muscle. Some hints have been found in studies on the development of cardiac hypertrophy, where two Ca2+-dependent enzymes are key actors: Ca2+/Calmodulin kinase II (CaMKII and phosphatase calcineurin, both of which are activated by the complex Ca2+/ /Calmodulin. The question now is how ET coupling occurs in cardiomyocytes, where intracellular Ca2+ is continuously oscillating. In this focused review, we will draw attention to location of Ca2+ signaling: intranuclear ([Ca2+]n or cytoplasmic ([Ca2+]c, and the specific ionic channels involved in the activation of cardiac ET coupling. Specifically, we will highlight the role of the 1,4,5 inositol triphosphate receptors (IP3Rs in the elevation of [Ca2+]n levels, which are important to locally activate CaMKII, and the role of transient receptor potential channels canonical (TRPCs in [Ca2+]c, needed to activate calcineurin.

  14. Elementary properties of CaV1.3 Ca(2+) channels expressed in mouse cochlear inner hair cells.

    Science.gov (United States)

    Zampini, Valeria; Johnson, Stuart L; Franz, Christoph; Lawrence, Neil D; Münkner, Stefan; Engel, Jutta; Knipper, Marlies; Magistretti, Jacopo; Masetto, Sergio; Marcotti, Walter

    2010-01-01

    Mammalian cochlear inner hair cells (IHCs) are specialized to process developmental signals during immature stages and sound stimuli in adult animals. These signals are conveyed onto auditory afferent nerve fibres. Neurotransmitter release at IHC ribbon synapses is controlled by L-type Ca(V)1.3 Ca(2+) channels, the biophysics of which are still unknown in native mammalian cells. We have investigated the localization and elementary properties of Ca(2+) channels in immature mouse IHCs under near-physiological recording conditions. Ca(V)1.3 Ca(2+) channels at the cell pre-synaptic site co-localize with about half of the total number of ribbons present in immature IHCs. These channels activated at about 70 mV, showed a relatively short first latency and weak inactivation, which would allow IHCs to generate and accurately encode spontaneous Ca(2+) action potential activity characteristic of these immature cells. The Ca(V)1.3 Ca(2+) channels showed a very low open probability (about 0.15 at 20 mV: near the peak of an action potential). Comparison of elementary and macroscopic Ca(2+) currents indicated that very few Ca(2+) channels are associated with each docked vesicle at IHC ribbon synapses. Finally, we found that the open probability of Ca(2+) channels, but not their opening time, was voltage dependent. This finding provides a possible correlation between presynaptic Ca(2+) channel properties and the characteristic frequency/amplitude of EPSCs in auditory afferent fibres.

  15. Variability of Mg/Ca and Sr/Ca between and within the planktonic foraminifers Globigerina bulloides and Globorotalia truncatulinoides

    NARCIS (Netherlands)

    Anand-Jha, P.; Elderfield, H.

    2005-01-01

    The variability in Mg/Ca and Sr/Ca within individual tests and chambers of tests, and samples consisting of differing numbers of individuals, was determined for Globigerina bulloides and Globorotalia truncatulinoides in a North Atlantic core top sample (52.918°N 16.917°W'). The variability in Mg/Ca

  16. Predictors of pretreatment CA125 at ovarian cancer diagnosis

    DEFF Research Database (Denmark)

    Babic, Ana; Cramer, Daniel W; Kelemen, Linda E

    2017-01-01

    in CA125 between studies and linear regression to estimate the association between epidemiologic factors and tumor characteristics and pretreatment CA125 levels. RESULTS: In age-adjusted models, older age, history of pregnancy, history of tubal ligation, family history of breast cancer, and family...... history of ovarian cancer were associated with higher CA125 levels while endometriosis was associated with lower CA125 levels. After adjusting for tumor-related characteristics (stage, histology, grade), body mass index (BMI) higher than 30 kg/m2was associated with 10% (95% CI 2, 19%) higher CA125 levels...

  17. P and Ca requirements for Japanese quail.

    Science.gov (United States)

    Mariz, C B L; Silva, J H V; Filho, J J; Lima, M R; Costa, F G P

    2017-04-01

    Four experiments were conducted to estimate the phosphorus and calcium requirements for weight maintenance and weight gain in Japanese quails during their growth phase from 16 to 36 days. Japanese quails aged 16 days were used for estimating the phosphorous and calcium requirements for weight maintenance or weight gain, with these quails composing each reference slaughter group and the others distributed in a completely randomized design, housed in cages of galvanized wire (33 × 33 × 16 cm) that were stored in acclimatized chambers with specific environmental temperatures. The light programme used during the 20-day experimental period was 24 h of artificial light. Analysis of the data showed that the prediction equations for estimating the phosphorus and calcium requirements for weight maintenance and weight gain of Japanese quails between 16 and 36 days of age were P (g/quail/day) = P 0.75 *(9.3695 + 7.7397*T) + 9.70*WG, in which P is the phosphorus requirement, and Ca (g/quail/day) = P 0.75 *(363.99 - 8.0262*T) + 28.15*WG, in which Ca is the calcium requirement, P is BW (kg), T is temperature (°C) and WG (g/quail/day). Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH.

  18. Klotho regulates CA1 hippocampal synaptic plasticity.

    Science.gov (United States)

    Li, Qin; Vo, Hai T; Wang, Jing; Fox-Quick, Stephanie; Dobrunz, Lynn E; King, Gwendalyn D

    2017-04-07

    Global klotho overexpression extends lifespan while global klotho-deficiency shortens it. As well, klotho protein manipulations inversely regulate cognitive function. Mice without klotho develop rapid onset cognitive impairment before they are 2months old. Meanwhile, adult mice overexpressing klotho show enhanced cognitive function, particularly in hippocampal-dependent tasks. The cognitive enhancing effects of klotho extend to humans with a klotho polymorphism that increases circulating klotho and executive function. To affect cognitive function, klotho could act in or on the synapse to modulate synaptic transmission or plasticity. However, it is not yet known if klotho is located at synapses, and little is known about its effects on synaptic function. To test this, we fractionated hippocampi and detected klotho expression in both pre and post-synaptic compartments. We find that loss of klotho enhances both pre and post-synaptic measures of CA1 hippocampal synaptic plasticity at 5weeks of age. However, a rapid loss of synaptic enhancement occurs such that by 7weeks, when mice are cognitively impaired, there is no difference from wild-type controls. Klotho overexpressing mice show no early life effects on synaptic plasticity, but decreased CA1 hippocampal long-term potentiation was measured at 6months of age. Together these data suggest that klotho affects cognition, at least in part, by regulating hippocampal synaptic plasticity. Copyright © 2017 IBRO. Published by Elsevier Ltd. All rights reserved.

  19. New fluorescent reagents specific for Ca2+-binding proteins

    International Nuclear Information System (INIS)

    Ben-Hail, Danya; Lemelson, Daniela; Israelson, Adrian; Shoshan-Barmatz, Varda

    2012-01-01

    Highlights: ► New reagents specifically inhibit the activity of Ca 2+ -dependent proteins. ► FITC-Ru and EITC-Ru allow for mechanism-independent probing of Ca 2+ -binding proteins. ► Changes in reagents fluorescence allow characterization of protein Ca 2+ -binding properties. -- Abstract: Ca 2+ carries information pivotal to cell life and death via its interactions with specific binding sites in a protein. We previously developed a novel photoreactive reagent, azido ruthenium (AzRu), which strongly inhibits Ca 2+ -dependent activities. Here, we synthesized new fluorescent ruthenium-based reagents containing FITC or EITC, FITC-Ru and EITC-Ru. These reagents were purified, characterized and found to specifically interact with and markedly inhibit Ca 2+ -dependent activities but not the activity of Ca 2+ -independent reactions. In contrast to many reagents that serve as probes for Ca 2+ , FITC-Ru and EITC-Ru are the first fluorescent divalent cation analogs to be synthesized and characterized that specifically bind to Ca 2+ -binding proteins and inhibit their activity. Such reagents will assist in characterizing Ca 2+ -binding proteins, thereby facilitating better understanding of the function of Ca 2+ as a key bio-regulator.

  20. Chronic Loss of CA2 Transmission Leads to Hippocampal Hyperexcitability.

    Science.gov (United States)

    Boehringer, Roman; Polygalov, Denis; Huang, Arthur J Y; Middleton, Steven J; Robert, Vincent; Wintzer, Marie E; Piskorowski, Rebecca A; Chevaleyre, Vivien; McHugh, Thomas J

    2017-05-03

    Hippocampal CA2 pyramidal cells project into both the neighboring CA1 and CA3 subfields, leaving them well positioned to influence network physiology and information processing for memory and space. While recent work has suggested unique roles for CA2, including encoding position during immobility and generating ripple oscillations, an interventional examination of the integrative functions of these connections has yet to be reported. Here we demonstrate that CA2 recruits feedforward inhibition in CA3 and that chronic genetically engineered shutdown of CA2-pyramidal-cell synaptic transmission consequently results in increased excitability of the recurrent CA3 network. In behaving mice, this led to spatially triggered episodes of network-wide hyperexcitability during exploration accompanied by the emergence of high-frequency discharges during rest. These findings reveal CA2 as a regulator of network processing in hippocampus and suggest that CA2-mediated inhibition in CA3 plays a key role in establishing the dynamic excitatory and inhibitory balance required for proper network function. Copyright © 2017 Elsevier Inc. All rights reserved.

  1. CA-125 in Disease Progression and Treatment of Lymphangioleiomyomatosis.

    Science.gov (United States)

    Glasgow, Connie G; Pacheco-Rodriguez, Gustavo; Steagall, Wendy K; Haughey, Mary E; Julien-Williams, Patricia A; Stylianou, Mario P; Gochuico, Bernadette R; Moss, Joel

    2018-02-01

    Lymphangioleiomyomatosis (LAM) is a destructive lung disease of women caused by proliferation of neoplastic-like LAM cells, with mutations in the TSC1/2 tumor suppressor genes. Based on case reports, levels of cancer antigen 125 (CA-125), an ovarian cancer biomarker, can be elevated in patients with LAM. We hypothesized that elevated serum CA-125 levels seen in some patients with LAM were due to LAM, not other malignancies, and might respond to sirolimus treatment. Serum CA-125 levels were measured for 241 patients at each visit. Medical records were reviewed for co-morbidities, disease progression, and response to sirolimus treatment. CA-125 expression in LAM cells was determined by using immunohistochemical analysis. Almost 25% of patients with LAM had at least one elevated serum CA-125 measurement. Higher serum CA-125 levels correlated with lower FEV 1 , premenopausal status, and pleural effusion in a multivariate model (each P CA-125 levels decreased following sirolimus treatment (P = .002). CA-125 and α-smooth muscle actin were co-expressed in LAM lung nodules. Higher serum CA-125 levels were associated with pleural effusions and reduced pulmonary function and were decreased with sirolimus therapy. LAM cells express CA-125. Some elevated serum CA-125 levels may reflect serosal membrane involvement. Copyright © 2017. Published by Elsevier Inc.

  2. TU-AB-BRA-12: Quality Assurance of An Integrated Magnetic Resonance Image Guided Adaptive Radiotherapy Machine Using Cherenkov Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Andreozzi, J; Bruza, P; Saunders, S; Pogue, B [Dartmouth College, Hanover, NH (United States); Mooney, K; Curcuru, A; Green, O [Washington University School of Medicine, Saint Louis, MO (United States); Gladstone, D [Dartmouth-Hitchcock Med. Ctr., Lebanon, NH (Lebanon)

    2016-06-15

    dose deposition. This study has been funded by NIH grants R21EB17559 and R01CA109558, as well as Norris Cotton Cancer Center Pilot funding.

  3. TU-G-BRB-05: Panel Discussion: Clinical Trials in Proton and Ion Therapy - Are We Ready?

    Energy Technology Data Exchange (ETDEWEB)

    Schulte, R. [Loma Linda Univ. (United States)

    2015-06-15

    development and support. Research reported in this presentation is supported by the National Cancer Institute of the National; Institutes of Health under Award Number P20CA183640.

  4. TU-AB-303-04: Characterizing CT-Derived Mass Change of Non-Tumor Pathology During Lung Radiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Guy, C; Weiss, E; Jan, N; Hugo, G [Virginia Commonwealth University, Richmond, VA (United States); Christensen, G [University of Iowa, Iowa City, IA (United States)

    2015-06-15

    National Cancer Institute of the National Institutes of Health under Award Number R01CA166119. The authors have no conflicts of interest.

  5. Spectral components of cytosolic [Ca2+] spiking in neurons

    DEFF Research Database (Denmark)

    Kardos, J; Szilágyi, N; Juhász, G

    1998-01-01

    We show here, by means of evolutionary spectral analysis and synthesis of cytosolic Ca2+ ([Ca2+]c) spiking observed at the single cell level using digital imaging fluorescence microscopy of fura-2-loaded mouse cerebellar granule cells in culture, that [Ca2+]c spiking can be resolved into evolutio......We show here, by means of evolutionary spectral analysis and synthesis of cytosolic Ca2+ ([Ca2+]c) spiking observed at the single cell level using digital imaging fluorescence microscopy of fura-2-loaded mouse cerebellar granule cells in culture, that [Ca2+]c spiking can be resolved...... into evolutionary spectra of a characteristic set of frequencies. Non-delayed small spikes on top of sustained [Ca2+]c were synthesized by a main component frequency, 0.132+/-0.012 Hz, showing its maximal amplitude in phase with the start of depolarization (25 mM KCI) combined with caffeine (10 mM) application...

  6. Preparation of Heterogeneous CaO Catalysts for Biodiesel Production

    Science.gov (United States)

    Widayat, W.; Darmawan, T.; Hadiyanto, H.; Rosyid, R. Ar

    2017-07-01

    The objective of this research was to develop heterogeneous catalysts from three CaO sources for biodiesel synthesis. The CaO catalyst were prepared from limestone, calcium hydroxide and calciun carbonate with thermal processing in a muffle furnace at 900°C.. The results showed that CaO catalyst from limestone has better characteristic than catalyst from Calcium Hydroxide and Calcium Carbonate. From morphology testing, the CaO catalyst derived from limestone formed a crystal, while The X-ray difraction analysis showed that the amount of CaO contained in limestone was the highest among the others. The yield of biodiesel obtained from the experiment was 89.98% for the catalyst from limestone; 85.15% for the catalyst Ca (OH)2; and 78.71% for CaCO3 catalyst.

  7. Spectroscopy of neutron-deficient nuclei around 36Ca

    International Nuclear Information System (INIS)

    Buerger, A.; Stanoiu, M.; Azaiez, F.; Bourgeois, Ch.; Franchoo, S.; Ibrahim, F.; Verney, D.; Dombradi, Zs.; Algora, A.; Fueloep, Zs.; Sohler, D.; Al-Khatib, A.; Bringel, P.; Engelhardt, C.; Huebel, H.; Bastin, B.; Benzoni, G.; Borcea, R.; Rotaru, F.; Sorlin, O.

    2006-01-01

    An experiment was performed to extend the knowledge of excited states in neutron-deficient Ca isotopes. In particular, excited states in 36Ca were searched for to allow for a comparison with its stable mirror nucleus, 36S. Secondary beams of 37Ca and 36Ca were produced by fragmentation of a primary 40Ca beam with an energy of 95 · A MeV on the SISSI target at GANIL. A variety of nuclei around 36Ca has been produced in a secondary Be target by neutron and proton-removal at beam energies around 61 · A MeV. The produced nuclei were identified using the spectrometer SPEG, and prompt γ rays were measured with the Chateau de Cristal. A preliminary value for the energy of the first 2+ state of 36Ca has been determined

  8. Structure of the CaMKIIdelta/calmodulin complex reveals the molecular mechanism of CaMKII kinase activation.

    Directory of Open Access Journals (Sweden)

    Peter Rellos

    2010-07-01

    Full Text Available Long-term potentiation (LTP, a long-lasting enhancement in communication between neurons, is considered to be the major cellular mechanism underlying learning and memory. LTP triggers high-frequency calcium pulses that result in the activation of Calcium/Calmodulin (CaM-dependent kinase II (CaMKII. CaMKII acts as a molecular switch because it remains active for a long time after the return to basal calcium levels, which is a unique property required for CaMKII function. Here we describe the crystal structure of the human CaMKIIdelta/Ca2+/CaM complex, structures of all four human CaMKII catalytic domains in their autoinhibited states, as well as structures of human CaMKII oligomerization domains in their tetradecameric and physiological dodecameric states. All four autoinhibited human CaMKIIs were monomeric in the determined crystal structures but associated weakly in solution. In the CaMKIIdelta/Ca2+/CaM complex, the inhibitory region adopted an extended conformation and interacted with an adjacent catalytic domain positioning T287 into the active site of the interacting protomer. Comparisons with autoinhibited CaMKII structures showed that binding of calmodulin leads to the rearrangement of residues in the active site to a conformation suitable for ATP binding and to the closure of the binding groove for the autoinhibitory helix by helix alphaD. The structural data, together with biophysical interaction studies, reveals the mechanism of CaMKII activation by calmodulin and explains many of the unique regulatory properties of these two essential signaling molecules.This article can also be viewed as an enhanced version in which the text of the article is integrated with interactive 3-D representations and animated transitions. Please note that a web plugin is required to access this enhanced functionality. Instructions for the installation and use of the Web plugin are available in Text S1.

  9. Synaptic NMDA Receptor-Dependent Ca2+ Entry Drives Membrane Potential and Ca2+ Oscillations in Spinal Ventral Horn Neurons

    Science.gov (United States)

    Alpert, Michael H.; Alford, Simon

    2013-01-01

    During vertebrate locomotion, spinal neurons act as oscillators when initiated by glutamate release from descending systems. Activation of NMDA receptors initiates Ca2+-mediated intrinsic membrane potential oscillations in central pattern generator (CPG) neurons. NMDA receptor-dependent intrinsic oscillations require Ca2+-dependent K+ (KCa2) channels for burst termination. However, the location of Ca2+ entry mediating KCa2 channel activation, and type of Ca2+ channel – which includes NMDA receptors and voltage-gated Ca2+ channels (VGCCs) – remains elusive. NMDA receptor-dependent Ca2+ entry necessitates presynaptic release of glutamate, implying a location at active synapses within dendrites, whereas VGCC-dependent Ca2+ entry is not similarly constrained. Where Ca2+ enters relative to KCa2 channels is crucial to information processing of synaptic inputs necessary to coordinate locomotion. We demonstrate that Ca2+ permeating NMDA receptors is the dominant source of Ca2+ during NMDA-dependent oscillations in lamprey spinal neurons. This Ca2+ entry is synaptically located, NMDA receptor-dependent, and sufficient to activate KCa2 channels at excitatory interneuron synapses onto other CPG neurons. Selective blockade of VGCCs reduces whole-cell Ca2+ entry but leaves membrane potential and Ca2+ oscillations unaffected. Furthermore, repetitive oscillations are prevented by fast, but not slow, Ca2+ chelation. Taken together, these results demonstrate that KCa2 channels are closely located to NMDA receptor-dependent Ca2+ entry. The close spatial relationship between NMDA receptors and KCa2 channels provides an intrinsic mechanism whereby synaptic excitation both excites and subsequently inhibits ventral horn neurons of the spinal motor system. This places the components necessary for oscillation generation, and hence locomotion, at glutamatergic synapses. PMID:23646190

  10. Błędy kształtu, porowatość wewnętrzna, dokładność wymiarów liniowych staliwnych odlewów precyzyjnych w aspekcie ekologii ich wytwarzania

    Directory of Open Access Journals (Sweden)

    J. Tomasik

    2012-12-01

    Full Text Available Dokonano kompleksowej oceny jakości odlewów precyzyjnych przez badanie warstwy wierzchniej, ocenę błędu kształtu, dokładnościwymiarów liniowych. Badania wykonano w oparciu o nowoczesną aparaturę, miedzy innymi zastosowano maszynę pomiarową ZeissCalypso.Otrzymane wyniki badań pozwolą na zastosowanie optymalnej technologii wytwarzania odlewów w procesie wytapianych modeliw aspekcie ekologii.

  11. Odpovědnost státu za škodu způsobenou rozhodnutím nebo nesprávným úředním postupem

    OpenAIRE

    Šimáková, Eva

    2012-01-01

    Odpovědnost státu za škodu způsobenou rozhodnutím nebo nesprávným úředním postupem Abstrakt English Thesis is interested in a legal institution of state liability for damage incurred due to the decision or incorrect official procedure as one of the particular case of a civil liability. It evaluates its legal forms on the one hand and specifics of proceedings concerning compensation for damage on the other, de lege lata in the area of the Czech national law. The thesis offers an European sight...

  12. Annotated draft genome sequences of three species of Cryptosporidium: Cryptosporidium meleagridis isolate UKMEL1, C. baileyi isolate TAMU-09Q1 and C. hominis isolates TU502_2012 and UKH1

    OpenAIRE

    Ifeonu, Olukemi O.; Chibucos, Marcus C.; Orvis, Joshua; Su, Qi; Elwin, Kristin; Guo, Fengguang; Zhang, Haili; Xiao, Lihua; Sun, Mingfei; Chalmers, Rachel M.; Fraser, Claire M.; Zhu, Guan; Kissinger, Jessica C.; Widmer, Giovanni; Silva, Joana C.

    2016-01-01

    Human cryptosporidiosis is caused primarily by Cryptosporidium hominis, C. parvum and C. meleagridis. To accelerate research on parasites in the genus Cryptosporidium, we generated annotated, draft genome sequences of human C. hominis isolates TU502_2012 and UKH1, C. meleagridis UKMEL1, also isolated from a human patient, and the avian parasite C. baileyi TAMU-09Q1. The annotation of the genome sequences relied in part on RNAseq data generated from the oocyst stage of both C. hominis and C. b...

  13. Mitochondria Maintain Distinct Ca2+Pools in Cone Photoreceptors.

    Science.gov (United States)

    Giarmarco, Michelle M; Cleghorn, Whitney M; Sloat, Stephanie R; Hurley, James B; Brockerhoff, Susan E

    2017-02-22

    Ca 2+ ions have distinct roles in the outer segment, cell body, and synaptic terminal of photoreceptors. We tested the hypothesis that distinct Ca 2+ domains are maintained by Ca 2+ uptake into mitochondria. Serial block face scanning electron microscopy of zebrafish cones revealed that nearly 100 mitochondria cluster at the apical side of the inner segment, directly below the outer segment. The endoplasmic reticulum surrounds the basal and lateral surfaces of this cluster, but does not reach the apical surface or penetrate into the cluster. Using genetically encoded Ca 2+ sensors, we found that mitochondria take up Ca 2+ when it accumulates either in the cone cell body or outer segment. Blocking mitochondrial Ca 2+ uniporter activity compromises the ability of mitochondria to maintain distinct Ca 2+ domains. Together, our findings indicate that mitochondria can modulate subcellular functional specialization in photoreceptors. SIGNIFICANCE STATEMENT Ca 2+ homeostasis is essential for the survival and function of retinal photoreceptors. Separate pools of Ca 2+ regulate phototransduction in the outer segment, metabolism in the cell body, and neurotransmitter release at the synaptic terminal. We investigated the role of mitochondria in compartmentalization of Ca 2+ We found that mitochondria form a dense cluster that acts as a diffusion barrier between the outer segment and cell body. The cluster is surprisingly only partially surrounded by the endoplasmic reticulum, a key mediator of mitochondrial Ca 2+ uptake. Blocking the uptake of Ca 2+ by mitochondria causes redistribution of Ca 2+ throughout the cell. Our results show that mitochondrial Ca 2+ uptake in photoreceptors is complex and plays an essential role in normal function. Copyright © 2017 the authors 0270-6474/17/372061-12$15.00/0.

  14. Tu Salud ¡Sí Cuenta! Your Health Matters! A Community-wide Campaign in a Hispanic Border Community in Texas.

    Science.gov (United States)

    Heredia, Natalia I; Lee, MinJae; Mitchell-Bennett, Lisa; Reininger, Belinda M

    To evaluate a community-wide campaign, Tu Salud ¡Si Cuenta! (TSSC), in improving eating behaviors and anthropometric outcomes in Hispanic border communities. A quasi-experimental study with matched intervention and comparison communities. Cross-sectional assessments with randomly sampled adults, examined by actual exposure and site (unexposed intervention, exposed intervention, and unexposed comparison). Predominately Mexican Americans located in Brownsville, TX (intervention) and Laredo, TX (control). The TSSC campaign included television and radio segments, community health worker discussions, and newsletters delivered in Brownsville from 2005 to 2010. Healthy and unhealthy eating indices and average hip and waist circumferences. Univariable and multivariable regression models. The sample (n = 799; 400 comparison and 399 intervention) was 98% of Mexican origin; 54% had completed grade 9 or higher. Exposure to any TSSC component was associated with a lower rate of unhealthy food consumption. Compared with the unexposed intervention group, the exposed intervention for the newsletter had a higher rate of healthy eating (adjusted rate ratio = 1.18; P < .01). Compared with the unexposed intervention, the exposed intervention for the community health worker discussion had a smaller hip circumference (adjusted mean difference = -5.77 cm; P < .05) and a smaller waist circumference (adjusted mean difference = -5.25 cm; P < .05). This study provides evidence for the use of community-wide campaigns for nutrition and obesity-related outcomes in Hispanic communities. Copyright © 2017 Society for Nutrition Education and Behavior. Published by Elsevier Inc. All rights reserved.

  15. Ca²+/calmodulin-dependent protein kinase II (CaMKII activity and sinoatrial nodal pacemaker cell energetics.

    Directory of Open Access Journals (Sweden)

    Yael Yaniv

    Full Text Available : Ca(2+-activated basal adenylate cyclase (AC in rabbit sinoatrial node cells (SANC guarantees, via basal cAMP/PKA-calmodulin/CaMKII-dependent protein phosphorylation, the occurrence of rhythmic, sarcoplasmic-reticulum generated, sub-membrane Ca(2+ releases that prompt rhythmic, spontaneous action potentials (APs. This high-throughput signaling consumes ATP.We have previously demonstrated that basal AC-cAMP/PKA signaling directly, and Ca(2+ indirectly, regulate mitochondrial ATP production. While, clearly, Ca(2+-calmodulin-CaMKII activity regulates ATP consumption, whether it has a role in the control of ATP production is unknown.We superfused single, isolated rabbit SANC at 37°C with physiological saline containing CaMKII inhibitors, (KN-93 or autocamtide-2 Related Inhibitory Peptide (AIP, or a calmodulin inhibitor (W-7 and measured cytosolic Ca(2+, flavoprotein fluorescence and spontaneous AP firing rate. We measured cAMP, ATP and O2 consumption in cell suspensions. Graded reductions in basal CaMKII activity by KN-93 (0.5-3 µmol/L or AIP (2-10 µmol/L markedly slow the kinetics of intracellular Ca(2+ cycling, decrease the spontaneous AP firing rate, decrease cAMP, and reduce O2 consumption and flavoprotein fluorescence. In this context of graded reductions in ATP demand, however, ATP also becomes depleted, indicating reduced ATP production.CaMKII signaling, a crucial element of normal automaticity in rabbit SANC, is also involved in SANC bioenergetics.

  16. L-type Ca2+ channel blockers promote Ca2+ accumulation when dopamine receptors are activated in striatal neurons.

    Science.gov (United States)

    Eaton, Molly E; Macías, Wendy; Youngs, Rachael M; Rajadhyaksha, Anjali; Dudman, Joshua T; Konradi, Christine

    2004-11-24

    Dopamine (DA) receptor-mediated signal transduction and gene expression play a central role in many brain disorders from schizophrenia to Parkinson's disease to addiction. While trying to evaluate the role of L-type Ca2+ channels in dopamine D1 receptor-mediated phosphorylation of the transcription factor cyclic AMP response element-binding protein (CREB), we found that activation of dopamine D1 receptors alters the properties of L-type Ca2+ channel inhibitors and turns them into facilitators of Ca2+ influx. In D1 receptor-stimulated neurons, L-type Ca2+ channel blockers promote cytosolic Ca2+ accumulation. This leads to the activation of a molecular signal transduction pathway and CREB phosphorylation. In the absence of dopamine receptor stimulation, L-type Ca2+ channel blockers inhibit CREB phosphorylation. The effect of dopamine on L-type Ca2+ channel blockers is dependent on protein kinase A (PKA), suggesting that protein phosphorylation plays a role in this phenomenon. Because of the adverse effect of activated dopamine receptors on L-type Ca2+ channel blocker action, the role of L-type Ca2+ channels in the dopamine D1 receptor signal transduction pathway cannot be assessed with pharmacological tools. However, with antisense technology, we demonstrate that L-type Ca2+ channels contribute to D1 receptor-mediated CREB phosphorylation. We conclude that the D1 receptor signal transduction pathway depends on L-type Ca2+ channels to mediate CREB phosphorylation.

  17. Synthesis of Al₂Ca Dispersoids by Powder Metallurgy Using a Mg-Al Alloy and CaO Particles.

    Science.gov (United States)

    Fujita, Junji; Umeda, Junko; Kondoh, Katsuyoshi

    2017-06-28

    The elemental mixture of Mg-6 wt %Al-1 wt %Zn-0.3 wt %Mn (AZ61B) alloy powder and CaO particles was consolidated by an equal-channel angular bulk mechanical alloying (ECABMA) process to form a composite precursor. Subsequently, the precursor was subjected to a heat treatment to synthesize fine Al₂Ca particles via a solid-state reaction between the Mg-Al matrix and CaO additives. Scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS) and electron probe micro-analysis on the precursor indicated that 4.7-at % Al atoms formed a supersaturated solid solution in the α-Mg matrix. Transmission electron microscopy-EDS and X-ray diffraction analyses on the AZ61B composite precursor with 10-vol % CaO particles obtained by heat treatment confirmed that CaO additives were thermally decomposed in the Mg-Al alloy, and the solid-soluted Ca atoms diffused along the α-Mg grain boundaries. Al atoms also diffused to the grain boundaries because of attraction to the Ca atoms resulting from a strong reactivity between Al and Ca. As a result, needle-like (Mg,Al)₂Ca intermetallics were formed as intermediate precipitates in the initial reaction stage during the heat treatment. Finally, the precipitates were transformed into spherical Al₂Ca particles by the substitution of Al atoms for Mg atoms in (Mg,Al)₂Ca after a long heat treatment.

  18. PGC-1{alpha} accelerates cytosolic Ca{sup 2+} clearance without disturbing Ca{sup 2+} homeostasis in cardiac myocytes

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Min, E-mail: chenminyx@gmail.com [Institute of Molecular Medicine, State Key Laboratory of Biomembrane and Membrane Biotechnology, Peking University, Beijing 100871 (China); Yunnan Centers for Diseases Prevention and Control, Kunming 650022 (China); Wang, Yanru [Institute of Molecular Medicine, State Key Laboratory of Biomembrane and Membrane Biotechnology, Peking University, Beijing 100871 (China); Qu, Aijuan [Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 (United States)

    2010-06-11

    Energy metabolism and Ca{sup 2+} handling serve critical roles in cardiac physiology and pathophysiology. Peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1{alpha}) is a multi-functional coactivator that is involved in the regulation of cardiac mitochondrial functional capacity and cellular energy metabolism. However, the regulation of PGC-1{alpha} in cardiac Ca{sup 2+} signaling has not been fully elucidated. To address this issue, we combined confocal line-scan imaging with off-line imaging processing to characterize calcium signaling in cultured adult rat ventricular myocytes expressing PGC-1{alpha} via adenoviral transduction. Our data shows that overexpressing PGC-1{alpha} improved myocyte contractility without increasing the amplitude of Ca{sup 2+} transients, suggesting that myofilament sensitivity to Ca{sup 2+} increased. Interestingly, the decay kinetics of global Ca{sup 2+} transients and Ca{sup 2+} waves accelerated in PGC-1{alpha}-expressing cells, but the decay rate of caffeine-elicited Ca{sup 2+} transients showed no significant change. This suggests that sarcoplasmic reticulum (SR) Ca{sup 2+}-ATPase (SERCA2a), but not Na{sup +}/Ca{sup 2+} exchange (NCX) contribute to PGC-1{alpha}-induced cytosolic Ca{sup 2+} clearance. Furthermore, PGC-1{alpha} induced the expression of SERCA2a in cultured cardiac myocytes. Importantly, overexpressing PGC-1{alpha} did not disturb cardiac Ca{sup 2+} homeostasis, because SR Ca{sup 2+} load and the propensity for Ca{sup 2+} waves remained unchanged. These data suggest that PGC-1{alpha} can ameliorate cardiac Ca{sup 2+} cycling and improve cardiac work output in response to physiological stress. Unraveling the PGC-1{alpha}-calcium handing pathway sheds new light on the role of PGC-1{alpha} in the therapy of cardiac diseases.

  19. Usefulness of CA 130 kit based on IRMA

    International Nuclear Information System (INIS)

    Fujii, Takashi; Kimura, Yoshiko; Ata, Mariko; Miyagawa, Naoko; Iio, Atsushi; Hamamoto, Ken

    1988-01-01

    Immunoradiometric assay for CA 130 was fundamentally and clinically evaluated using a commercially available D-7111 kit. Incubation time was 4 hr with the present CA 133 kit as compared with 16 - 24 hr with conventional CA 125 kit. Laboratory performance of CA 130 kit was satisfactory for standard curve, reproducibility, and recovery test. There was well correlation between the present CA 130 kit and CA 125 kit (r = 0.931). The concentration of CA 130 in the serum was significantly higher in healthy women than men (17.3 +- 10.5 U/ml vs 9.6 +- 5.1 U/ml). Serum CA 130 levels tended to decrease with aging, regardless of sex. These levels were changeable with menstrual cycle ; i.e., these were significantly higher during menstrual phase (24.2 +- 9.0 U/ml) and significantly lower during ovulatory phase (10.9 +- 2.4 U/ml) and during menopause (12.1 +- 3.4 U/ml). Cut off serum CA 130 levels were defined as 20 U/ml for men and 38 U/ml for women. Positive rate for CA 130 was the highest in cases of ovarian cancer (80 %), followed by endometrial cancer (50 %), pancreatic cancer (47 %), benign ovarian tumor (44 %), and lung cancer (39 %). (Namekawa, K.)

  20. The relationship between ovarian volume and serum CA-125 levels.

    Science.gov (United States)

    Gulec, U K; Paydas, S; Guzel, A B; Vardar, M A; Urunsak, I F; Cetin, M T

    2014-01-01

    The aim of this study was to investigate the relationship between ovarian volume and serum CA-125 levels. Serum CA-125 levels and ovarian volume were compared among the cases with benign ovarian neoplasms, primary epithelial ovarian cancer (EOC), controlled ovarian hyperstimulation, and ovarian hyperstimulation syndrome (OHSS). Also, the correlation between CA-125 levels and ovarian volume were evaluated in the presence of peritoneal fluid and/or peritoneal carcinomatosis. Although ovarian volume was not different among the groups, CA-125 levels were higher in the cases with EOC than with benign ovarian tumors (p = 0.001). Baseline CA-125 levels were not found to have increased while ovarian volume went up with controlled hyperstimulation in the infertile group (p = 0.555). However, uncontrolled hyperstimulation of the ovaries and the presence of peritoneal fluid caused an increase in the levels of CA-125 (p = 0.001). There was no correlation between ovarian volume and CA-125 levels in the cases with malignant ovarian tumors (r = 0.083). The results of this study have confirmed that CA-125 is a peritoneal marker and increased ovarian volume with benign ovarian neoplasms or controlled hyperstimulation does not increase CA-125 levels in the same way. The presence of peritoneal carcinomatosis and/or peritoneal fluid seems to be an important factor for high CA-125 levels in patients with epithelial ovarian cancer (EOC).

  1. FAST INVERSION OF SOLAR Ca II SPECTRA

    International Nuclear Information System (INIS)

    Beck, C.; Choudhary, D. P.; Rezaei, R.; Louis, R. E.

    2015-01-01

    We present a fast (<<1 s per profile) inversion code for solar Ca II lines. The code uses an archive of spectra that are synthesized prior to the inversion under the assumption of local thermodynamic equilibrium (LTE). We show that it can be successfully applied to spectrograph data or more sparsely sampled spectra from two-dimensional spectrometers. From a comparison to a non-LTE inversion of the same set of spectra, we derive a first-order non-LTE correction to the temperature stratifications derived in the LTE approach. The correction factor is close to unity up to log τ ∼ –3 and increases to values of 2.5 and 4 at log τ = –6 in the quiet Sun and the umbra, respectively

  2. Radiolise de Ca14Co3

    OpenAIRE

    Maria Guadalupe Albarran Sanchez

    1986-01-01

    Resumo: A ação da radiação sobre carbonatos sólidos produz várias espécies. A dissolução do sólido resulta na formação de alguns compostos orgânicos que podem ser observados por diferentes métodos químicos. Dentro destes métodos, o da cromatografia líquida de partição/exclusão de íons tem sido utilizada para análise dos ácidos carboxílicos produzidos. Nosso estudo consistiu em reexaminar este método de análise, estendendo-o para possíveis compostos não ionizáveis obtidos na radiólise de CaCO3...

  3. CEA a CA 19-9 při monitorování nemocných s nádory kolorekta

    Czech Academy of Sciences Publication Activity Database

    Šimíčková, M.; Pecen, Ladislav; Nekulová, M.; Vondráček, V.

    č. 1 (2001), s. 4-6 R&D Projects: GA AV ČR KSK1019101 Institutional research plan: AV0Z1030915 Keywords : recurrence prediction * colorectal tu * tumor markers Subject RIV: BB - Applied Statistics, Operational Research

  4. Hydroxylated xestospongins block inositol-1,4,5-trisphosphate-induced Ca2+ release and sensitize Ca2+-induced Ca2+ release mediated by ryanodine receptors.

    Science.gov (United States)

    Ta, Tram Anh; Feng, Wei; Molinski, Tadeusz F; Pessah, Isaac N

    2006-02-01

    Inositol-1,4,5-trisphosphate receptors (IP(3)Rs) and ryanodine receptors (RyRs) often coexist within the endoplasmic/sarcoplasmic reticulum (ER/SR) membrane and coordinate precise spatial and temporal coding of Ca(2+) signals in most animal cells. Xestospongin C (XeC) was shown to selectively block IP(3)-induced Ca(2+) release and IP(3)R-mediated signaling (Gafni et al., 1997). We have further studied the specificity of xestospongin structures possessing ring hydroxyl (-OH) substituents toward IP(3)R, RyR, and ER/SR Ca(2+)-ATPase (SERCA) activities. XeC potently inhibits IP(3)R, weakly inhibits RyR1, and lacks activity toward SERCA1 and SERCA2. XeD (9-OH XeC), 7-OH-XeA, and araguspongin C isolated from the marine sponge Xestospongia species also inhibit IP(3)-mediated Ca(2+) release and lack activity toward SERCA. However, these hydroxylated derivatives possess a unique activity in that they enhance Ca(2+)-induced Ca(2+) release from SR vesicles by a mechanism involving the sensitization of RyR1 channels within the same concentration range needed to block IP(3)-induced Ca(2+) release. These results show that xestospongins and related structures lack direct SERCA inhibitory activity, as suggested by some previous studies. A new finding is that XeD and related structures possessing a hydroxylated oxaquinolizidine ring are IP(3)R blockers that also enhance Ca(2+)-induced Ca(2+) release mediated by RyRs. In intact cells, the actions of XeD are blocked by ryanodine pretreatment and do not interfere with thapsigargin-mediated Ca(2+) mobilization stemming from SERCA block. Hydroxylated bis-oxaquinolizadine derivatives isolated from Xestospongia species are novel bifunctional reagents that may be useful in ascertaining how IP(3)Rs and RyRs contribute to cell signaling.

  5. The many phases of CaC{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Konar, Sumit; Nylén, Johanna; Svensson, Gunnar; Bernin, Diana; Edén, Mattias [Department of Materials and Environmental Chemistry, Stockholm University, SE-10691 Stockholm (Sweden); Ruschewitz, Uwe [Department of Chemistry, University of Cologne, Greinstrasse 6, 50939 Cologne (Germany); Häussermann, Ulrich, E-mail: Ulrich.Haussermann@mmk.su.se [Department of Materials and Environmental Chemistry, Stockholm University, SE-10691 Stockholm (Sweden)

    2016-07-15

    Polymorphic CaC{sub 2} was prepared by reacting mixtures of CaH{sub 2} and graphite with molar ratios between 1:1.8 and 1:2.2 at temperatures between 700 and 1400 °C under dynamic vacuum. These conditions provided a well controlled, homogeneous, chemical environment and afforded products with high purity. The products, which were characterized by powder X-ray diffraction, solid state NMR and Raman spectroscopy, represented mixtures of the three known polymorphs, tetragonal CaC{sub 2}-I and monoclinic CaC{sub 2}-II and -III. Their proportion is dependent on the nominal C/CaH{sub 2} ratio of the reaction mixture and temperature. Reactions with excess carbon produced a mixture virtually free from CaC{sub 2}-I, whereas high temperatures (above 1100 °C) and C-deficiency favored the formation of CaC{sub 2}-I. From first principles calculations it is shown that CaC{sub 2}-I is dynamically unstable within the harmonic approximation. This indicates that existing CaC{sub 2}-I is structurally/dynamically disordered and may possibly even occur as slightly carbon-deficient phase CaC{sub 2-δ}. It is proposed that monoclinic II is the ground state of CaC{sub 2} and polymorph III is stable at temperatures above 200 °C. Tetragonal I represents a metastable, heterogeneous, phase of CaC{sub 2}. It is argued that a complete understanding of the occurrence of three room temperature modifications of CaC{sub 2} will require a detailed characterization of compositional and structural heterogeneities within the high temperature form CaC{sub 2}-IV, which is stable above 450 °C. The effect of high pressure on the stability of the monoclinic forms of CaC{sub 2} was studied in a diamond anvil cell using Raman spectroscopy. CaC{sub 2}-II and -III transform into tetragonal CaC{sub 2}-I at about 4 and 1GPa, respectively. - Graphical abstract: The famous tetragonal form of CaC{sub 2}, CaC{sub 2}-I, is dynamically unstable. Why does it exist? Display Omitted - Highlights: • Critical review

  6. Enhanced Ca(2+)-activated Na(+)-Ca(2+) exchange activity in canine pacing-induced heart failure.

    Science.gov (United States)

    Hobai, I A; O'Rourke, B

    2000-10-13

    Defective excitation-contraction coupling in heart failure is generally associated with both a reduction in sarcoplasmic reticulum (SR) Ca(2+) uptake and a greater dependence on transsarcolemmal Na(+)-Ca(2+) exchange (NCX) for Ca(2+) removal. Although a relative increase in NCX is expected when SR function is impaired, few and contradictory studies have addressed whether there is an absolute increase in NCX activity. The present study examines in detail NCX density and function in left ventricular midmyocardial myocytes isolated from normal or tachycardic pacing-induced failing canine hearts. No change of NCX current density was evident in myocytes from failing hearts when intracellular Ca(2+) ([Ca(2+)](i)) was buffered to 200 nmol/L. However, when [Ca(2+)](i) was minimally buffered with 50 micromol/L indo-1, Ca(2+) extrusion via NCX during caffeine application was doubled in failing versus normal cells. In other voltage-clamp experiments in which SR uptake was blocked with thapsigargin, both reverse-mode and forward-mode NCX currents and Ca(2+) transport were increased >2-fold in failing cells. These results suggest that, in addition to a relative increase in NCX function as a consequence of defective SR Ca(2+) uptake, there is an absolute increase in NCX function that depends on [Ca(2+)](i) in the failing heart.

  7. Selenoprotein W redox-regulated Ca2+ channels correlate with selenium deficiency-induced muscles Ca2+ leak.

    Science.gov (United States)

    Yao, Haidong; Fan, Ruifeng; Zhao, Xia; Zhao, Wenchao; Liu, Wei; Yang, Jie; Sattar, Hamid; Zhao, Jinxin; Zhang, Ziwei; Xu, Shiwen

    2016-09-06

    Selenium (Se) deficiency induces Ca2+ leak and calcification in mammal skeletal muscles; however, the exact mechanism is still unclear. In the present study, both Se-deficient chicken muscle models and selenoprotein W (SelW) gene knockdown myoblast and embryo models were used to study the mechanism. The results showed that Se deficiency-induced typical muscular injuries accompanied with Ca2+ leak and oxidative stress (P CACNA1S, ORAI1, STIM1, TRPC1, and TRPC3 (P CACNA1S, ORA1, TRPC1, and TRPC3; and caused abnormal activities of Ca2+ channels in response to inhibitors in myoblasts and chicken embryos. Thus, both Se deficiency and SelW knockdown induced Ca2+ leak, oxidative stress, and Ca2+ channel reduction. In addition, Ca2+ levels and the expression of the Ca2+ channels, RyR1, SERCA, CACNA1S, TRPC1, and TRPC3 were recovered to normal levels by N-acetyl-L-cysteine (NAC) treatment compared with SelW knockdown cells. Thus, with regard to the decreased Ca2+ channels, SelW knockdown closely correlated Se deficiency with Ca2+ leak in muscles. The redox regulation role of SelW is crucial in Se deficiency-induced Ca2+ leak in muscles.

  8. Serum tetranectin and CA125 in endometrial adenocarcinoma

    DEFF Research Database (Denmark)

    Lundstrøm, M S; Høgdall, C K; Nielsen, Anette Lynge

    2000-01-01

    BACKGROUND: CA125 and tetranectin (TN) are prognostic markers in ovarian cancer. This study examines the values of these markers in endometrial cancer. MATERIALS AND METHODS: TN and CA125 were determined preoperatively in 99 patients with primary endometrioid adenocarcinoma and evaluated...... in relation to tumor grade, stage and cancer survival. RESULTS: The CA125 levels correlated significantly with tumor stage. Dichotomized according to a cut-off level of 35 U/ml, CA125 significantly correlated with cancer death. Multivariate regression analysis of cancer survival time showed that CA125 > 35 U....../ml was not an independent factor when stage was introduced. TN levels were within the normal range in all patients and did not show any association with tumor grade, stage or survival. CONCLUSIONS: The study confirmed the role of CA125 as a prognostic factor in endometrial cancer and may be of aid in pointing out patients...

  9. Residual sarcoplasmic reticulum Ca2+ concentration after Ca2+ release in skeletal myofibers from young adult and old mice.

    Science.gov (United States)

    Wang, Zhong-Min; Tang, Shen; Messi, María Laura; Yang, Jenny J; Delbono, Osvaldo

    2012-04-01

    Contrasting information suggests either almost complete depletion of sarcoplasmic reticulum (SR) Ca(2+) or significant residual Ca(2+) concentration after prolonged depolarization of the skeletal muscle fiber. The primary obstacle to resolving this controversy is the lack of genetically encoded Ca(2+) indicators targeted to the SR that exhibit low-Ca(2+) affinity, a fast biosensor: Ca(2+) off-rate reaction, and can be expressed in myofibers from adult and older adult mammalian species. This work used the recently designed low-affinity Ca(2+) sensor (Kd = 1.66 mM in the myofiber) CatchER (calcium sensor for detecting high concentrations in the ER) targeted to the SR, to investigate whether prolonged skeletal muscle fiber depolarization significantly alters residual SR Ca(2+) with aging. We found CatchER a proper tool to investigate SR Ca(2+) depletion in young adult and older adult mice, consistently tracking SR luminal Ca(2+) release in response to brief and repetitive stimulation. We evoked SR Ca(2+) release in whole-cell voltage-clamped flexor digitorum brevis muscle fibers from young and old FVB mice and tested the maximal SR Ca(2+) release by directly activating the ryanodine receptor (RyR1) with 4-chloro-m-cresol in the same myofibers. Here, we report for the first time that the Ca(2+) remaining in the SR after prolonged depolarization (2 s) in myofibers from aging (~220 μM) was larger than young (~132 μM) mice. These experiments indicate that SR Ca(2+) is far from fully depleted under physiological conditions throughout life, and support the concept of excitation-contraction uncoupling in functional senescent myofibers.

  10. Kļūdu analīze Latvijas pilsētu tīmekļa vietņu tulkojumos vācu valodā

    OpenAIRE

    Surova, Jevgeņija

    2015-01-01

    Maģistra darba „Kļūdu analīze Latvijas pilsētu tīmekļa vietņu tulkojumos vācu valodā” mērķis ir sniegt ieguldījumu tūrisma jomas tekstu tulkošanas uzlabošanā, kā arī izstrādāt ieteikumus tūrisma tekstu tulkotājiem svešvalodā, norādot uz būtiskākajām kļūdām jeb nepilnībām. Šī mērķa sasniegšanai tika izvēlētas 10 Latvijas pilsētu un citas ar tūrismu saistītas tīmekļa vietnes, kuras pieejamas arī vācu valodā. Tulkojumi vācu valodā tika vērtēti un analizēti no vairākiem valodas un tulkošanas aspe...

  11. Banku loma noziedzīgi iegūtu līdzekļu legalizācijas un terorisma finansēšanas novēršanā

    OpenAIRE

    Beirote, Laura

    2017-01-01

    Bankas līdz pat šim brīdim ir bijušas lielākā privātā sektora pārstāves, kas ir veicinājušas naudas atmazgāšanas un terorisma finansēšanas atklāšanu, tādēļ autors ar šī darba palīdzību vēlas norādīt tieši uz banku nozīmīgo lomu noziedzīgi iegūtu līdzekļu legalizācijas un terorisma finansēšanas novēršanā. Valsts institūcijas nevar bez finanšu iestāžu starpniecības šādus nelikumīgus darījumus izsekot, un saukt pie kriminālās atbildības vainīgās personas. Darbā ir analizēts noziedzīgi iegūtu lī...

  12. Genotypic and phenotypic characterization of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex with the proposal of Acinetobacter pittii sp. nov. (formerly Acinetobacter genomic species 3) and Acinetobacter nosocomialis sp. nov. (formerly Acinetobacter genomic species 13TU).

    Science.gov (United States)

    Nemec, Alexandr; Krizova, Lenka; Maixnerova, Martina; van der Reijden, Tanny J K; Deschaght, Pieter; Passet, Virginie; Vaneechoutte, Mario; Brisse, Sylvain; Dijkshoorn, Lenie

    2011-05-01

    Acinetobacter genomic species (gen. sp.) 3 and gen. sp. 13TU are increasingly recognized as clinically important taxa within the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex. To define the taxonomic position of these genomic species, we investigated 80 strains representing the known diversity of the ACB complex. All strains were characterized by AFLP analysis, amplified rDNA restriction analysis and nutritional or physiological testing, while selected strains were studied by 16S rRNA and rpoB gene sequence analysis, multilocus sequence analysis and whole-genome comparison. Results supported the genomic distinctness and monophyly of the individual species of the ACB complex. Despite the high phenotypic similarity among these species, some degree of differentiation between them could be made on the basis of growth at different temperatures and of assimilation of malonate, l-tartrate levulinate or citraconate. Considering the medical relevance of gen. sp. 3 and gen. sp. 13TU, we propose the formal names Acinetobacter pittii sp. nov. and Acinetobacter nosocomialis sp. nov. for these taxa, respectively. The type strain of A. pittii sp. nov. is LMG 1035(T) (=CIP 70.29(T)) and that of A. nosocomialis sp. nov. is LMG 10619(T) (=CCM 7791(T)). Copyright © 2011 Institut Pasteur. Published by Elsevier SAS. All rights reserved.

  13. Improved custom statistics visualization for CA Performance Center data

    CERN Document Server

    Talevi, Iacopo

    2017-01-01

    The main goal of my project is to understand and experiment the possibilities that CA Performance Center (CA PC) offers for creating custom applications to display stored information through interesting visual means, such as maps. In particular, I have re-written some of the network statistics web pages in order to fetch data from new statistics modules in CA PC, which has its own API, and stop using the RRD data.

  14. Circularly permuted green fluorescent proteins engineered to sense Ca2+.

    Science.gov (United States)

    Nagai, T; Sawano, A; Park, E S; Miyawaki, A

    2001-03-13

    To visualize Ca(2+)-dependent protein-protein interactions in living cells by fluorescence readouts, we used a circularly permuted green fluorescent protein (cpGFP), in which the amino and carboxyl portions had been interchanged and reconnected by a short spacer between the original termini. The cpGFP was fused to calmodulin and its target peptide, M13. The chimeric protein, which we have named "pericam," was fluorescent and its spectral properties changed reversibly with the amount of Ca(2+), probably because of the interaction between calmodulin and M13 leading to an alteration of the environment surrounding the chromophore. Three types of pericam were obtained by mutating several amino acids adjacent to the chromophore. Of these, "flash-pericam" became brighter with Ca(2+), whereas "inverse-pericam" dimmed. On the other hand, "ratiometric-pericam" had an excitation wavelength changing in a Ca(2+)-dependent manner. All of the pericams expressed in HeLa cells were able to monitor free Ca(2+) dynamics, such as Ca(2+) oscillations in the cytosol and the nucleus. Ca(2+) imaging using high-speed confocal line-scanning microscopy and a flash-pericam allowed to detect the free propagation of Ca(2+) ions across the nuclear envelope. Then, free Ca(2+) concentrations in the nucleus and mitochondria were simultaneously measured by using ratiometric-pericams having appropriate localization signals, revealing that extra-mitochondrial Ca(2+) transients caused rapid changes in the concentration of mitochondrial Ca(2+). Finally, a "split-pericam" was made by deleting the linker in the flash-pericam. The Ca(2+)-dependent interaction between calmodulin and M13 in HeLa cells was monitored by the association of the two halves of GFP, neither of which was fluorescent by itself.

  15. SNL/CA Environmental Management System Program Manual.

    Energy Technology Data Exchange (ETDEWEB)

    Larsen, Barbara L.

    2007-04-01

    The Sandia National Laboratories, California (SNL/CA) Environmental Management System (EMS) Program Manual documents the elements of the site EMS Program. The SNL/CA EMS Program conforms to the International Standard on Environmental Management Systems, ISO 14001:2004. Elements of the ISO standard overlap with those of Department of Energy (DOE) Order 450.1, thus SNL/CA's EMS program also meets the DOE requirements.

  16. Combined Evaluation of AFP, CA15-3, CA125, CA19-9, and CEA Tumor Markers in Patients with Hepatitis B and C.

    Science.gov (United States)

    Assmar, Mehdi; Yeganeh, Sara; Mansourghanaei, Fariborz; Amirmozafari, Nour

    2016-12-01

    This study aimed to determine the role of tumor markers AFP, CA15-3, CA125, CA19-9 and CEA in patients with hepatitis B and C. This descriptive cross-sectional study was performed from Oct 2012 to Oct 2014. Serum samples of 129 patients with hepatitis B and C referred to Guilan Liver and Digestive Disease Research Center in Rasht, Iran were collected and checked for the existence of the listed tumor markers by ELISA. No increase in serum levels of tumor marker CA19-9, CEA and CA15-3 were seen in patients with hepatitis ( P >0.05). In patients with hepatitis B, increase in CA125 were observed ( P =0.03). In hepatitis C patients, there was an increase in AFP levels ( P =0.03). The levels of AFP and CA125 markers were high in hepatitis C and hepatitis B, respectively. However, the increased levels were not seen is malignancy. Due to the small sample size, further study is necessary to find the reasons of the increase.

  17. Influence of Ca substitution by Mg on the Ca3Co4O9 performances

    Directory of Open Access Journals (Sweden)

    Constantinescu, G.

    2014-02-01

    Full Text Available Ca3-xMgxCo4O9 polycrystalline thermoelectric ceramics with small amounts of Mg have been synthesized by the classical solid state method. Microstructural characterizations have shown that all the Mg has been incorporated into the Ca3Co4O9 structure and no Mg-based secondary phases have been identified. Apparent density measurements have shown that samples do not modify their density until 0.05 Mg content, decreasing for higher contents. Electrical resistivity decreases and Seebeck coefficient slightly raises when Mg content increases until 0.05 Mg addition. The improvement in both parameters leads to higher power factor values than the usually obtained in samples prepared by the conventional solid state routes and close to those obtained in textured materials.Se han preparado cerámicas termoeléctricas policristalinas de Ca3-xMgxCo4O9, con pequeñas cantidades de Mg, utilizando el método clásico de estado sólido. La caracterización microestructural ha mostrado que el Mg se ha incorporado en la estructura del Ca3Co4O9 y que no se han formado fases secundarias con el Mg. Las medidas de densidad aparente han mostrado que las muestras prácticamente poseen la misma densidad hasta un contenido de 0.05 Mg, disminuyendo para contenidos mayores. La resistividad eléctrica disminuye y el coeficiente Seebeck aumenta cuando el contenido de Mg se incrementa hasta valores de 0.05. La mejora de ambos parámetros conduce a valores del factor de potencia mayores que los obtenidos en muestras preparadas por la vía convencional de estado sólido, alcanzando valores próximos a los que se encuentran en materiales texturados.

  18. TU-E-201-03: Eye Lens Dosimetry in Radiotherapy Using Contact Lens-Shaped Applicator

    Energy Technology Data Exchange (ETDEWEB)

    Park, J. [Seoul National University Hospital (Korea, Republic of)

    2015-06-15

    awareness can lead to avoidance or even prevention. Learning Objectives: To understand recent changes in eye lens dose limits and thresholds for tissue reactions To understand different approaches to dose estimation for eye lens To learn about challenges in eye lens opacities among staff in interventional fluoroscopy Di Zhang, Toshiba America Medical Systems, Tustin, CA, USA Eye lens radiation dose from brain perfusion CT exams CT perfusion imaging requires repeatedly exposing one location of the head to monitor the uptake and washout of iodinated contrast. The accumulated radiation dose to the eye lens can be high, leading to concerns about potential radiation injury from these scans. CTDIvol assumes continuous z coverage and can overestimate eye lens dose in CT perfusion scans where the table do not increment. The radiation dose to the eye lens from clinical CT brain perfusion studies can be estimated using Monte Carlo simulation methods on voxelized patient models. MDCT scanners from four major manufacturers were simulated and the eye lens doses were estimated using the AAPM posted clinical protocols. They were also compared to CTDIvol values to evaluate the overestimation from CTDIvol. The efficacy of eye lens dose reduction techniques such as tilting the gantry and moving the scan location away from the eyelens were also investigated. Eye lens dose ranged from 81 mGy to 279 mGy, depending on the scanner and protocol used. It is between 59% and 63% of the CTDIvol values reported by the scanners. The eye lens dose is significantly reduced when the eye lenses were not directly irradiated. CTDIvol should not be interpreted as patient dose; this study has shown it to overestimate dose to the eye lens. These results may be used to provide more accurate estimates of actual dose to ensure that protocols are operated safely below thresholds. Tilting the gantry or moving the scanning region further away from the eyes are effective for reducing lens dose in clinical practice

  19. TU-E-201-01: Methods for Eye Lens Dosimetry and Studies On Lens Opacities with Interventionists

    Energy Technology Data Exchange (ETDEWEB)

    Rehani, M. [Massachusetts General Hospital (United States)

    2015-06-15

    awareness can lead to avoidance or even prevention. Learning Objectives: To understand recent changes in eye lens dose limits and thresholds for tissue reactions To understand different approaches to dose estimation for eye lens To learn about challenges in eye lens opacities among staff in interventional fluoroscopy Di Zhang, Toshiba America Medical Systems, Tustin, CA, USA Eye lens radiation dose from brain perfusion CT exams CT perfusion imaging requires repeatedly exposing one location of the head to monitor the uptake and washout of iodinated contrast. The accumulated radiation dose to the eye lens can be high, leading to concerns about potential radiation injury from these scans. CTDIvol assumes continuous z coverage and can overestimate eye lens dose in CT perfusion scans where the table do not increment. The radiation dose to the eye lens from clinical CT brain perfusion studies can be estimated using Monte Carlo simulation methods on voxelized patient models. MDCT scanners from four major manufacturers were simulated and the eye lens doses were estimated using the AAPM posted clinical protocols. They were also compared to CTDIvol values to evaluate the overestimation from CTDIvol. The efficacy of eye lens dose reduction techniques such as tilting the gantry and moving the scan location away from the eyelens were also investigated. Eye lens dose ranged from 81 mGy to 279 mGy, depending on the scanner and protocol used. It is between 59% and 63% of the CTDIvol values reported by the scanners. The eye lens dose is significantly reduced when the eye lenses were not directly irradiated. CTDIvol should not be interpreted as patient dose; this study has shown it to overestimate dose to the eye lens. These results may be used to provide more accurate estimates of actual dose to ensure that protocols are operated safely below thresholds. Tilting the gantry or moving the scanning region further away from the eyes are effective for reducing lens dose in clinical practice

  20. A new tumor marker: CA125 for ovarian carcinomas

    International Nuclear Information System (INIS)

    Sakahara, H.; Endo, K.; Nakajima, K.

    1985-01-01

    To evaluate CA125 as a tumor marker for ovarian carcinomas, CA125 concentrations were measured by the simultaneous immunoradiometric assay. The binding of I-125 labeled monoclonal antibody to the bead-bound antigen was greatly influenced by many factors, such as the incubation time, pH, IgG concentrations of samples, the sequence of addition of the tracer and samples and so on. By applying the forward two-step assay, diminished binding was observed than in the simultaneous assay, probably due to the relatively low affinity of the antibody. This simultaneous immunoradiometric assay resulted in the ''prozone'' or ''hook'' effect at high CA125 samples and proper dilution was necessary to determine the accurate CA125 values. All 72 normal control subjects had low concentrations of under 35 U/ml. Elevated serum CA125 was observed in 43% (9/21) cases with malignant ovarian tumors, depending on the stage and the histopathological findings. All 4 serous cystadenocarcinomas and 2 of 3 endometrioid carcinomas were positive and the measurement of serum CA125 was useful in the sequential monitoring of these cases. In contrast, 51 benign gynecological diseases, none had elevated serum CA125 except one with follicular cyst. Among 75 cases with non-gynecological benign and malignant diseases, only 1 of 12 gastric carcinomas and 2 of 13 pancreatic carcinomas had elevated CA125 levels. In summary, CA125 is a promising and relatively specific marker for ovarian carcinomas

  1. TRPV5-mediated Ca2+ Reabsorption and Hypercalciuria

    Science.gov (United States)

    Renkema, Kirsten Y.; Hoenderop, Joost G. J.; Bindels, René J. M.

    2007-04-01

    The concerted action of the intestine, kidney and bone results in the maintenance of a normal Ca2+ balance, a mechanism that is tightly controlled by the calciotropic hormones vitamin D, parathyroid hormone and calcitonin. Disturbances in the Ca2+ balance have been linked to diverse pathophysiological disorders like urolithiasis, hypertension, electroencephalogram abnormalities and rickets. Importantly, the final amount of Ca2+ that is released from the body is determined in the distal part of the nephron, where active Ca2+ reabsorption occurs. Here, Transient Receptor Potential Vanilloid member 5 (TRPV5), a highly Ca2+-selective channel, has been recognized as the gatekeeper of active Ca2+ reabsorption. The in vivo relevance of TRPV5 has been further investigated by the characterization of TRPV5 knockout (TRPV5-/-) mice, which exhibit severe disturbances in renal Ca2+ handling, such as profound hypercalciuria, intestinal Ca2+ hyperabsorption and reduced bone thickness. Hypercalciuria increases the risk of kidney stone formation in these mice. This review highlights our current knowledge about TRPV5-mediated Ca2+ reabsorption and emphasizes the physiological relevance and the clinical implications related to the TRPV5-/- mice model.

  2. Hydriding and dehydriding properties of CaSi

    International Nuclear Information System (INIS)

    Aoki, Masakazu; Ohba, Nobuko; Noritake, Tatsuo; Towata, Shin-ichi

    2005-01-01

    The hydriding and dehydriding properties of CaSi were investigated both theoretically and experimentally. First-principles calculations suggested that CaSiH n is thermodynamically stable. Experimentally, the p -c isotherms clearly demonstrated plateau pressures in a temperature range of 473-573 K and the maximum hydrogen content was 1.9 weight % (wt.%) under a hydrogen pressure of 9 MPa at 473 K. The structure of CaSiH n is different from those of ZrNi hydrides, although CaSi has the CrB-type structure as well as ZrNi

  3. β decay of neutron-rich 53-56Ca

    International Nuclear Information System (INIS)

    Mantica, P. F.; Crawford, H. L.; Damaske, A.; Pinter, J. S.; Stoker, J. B.; Broda, R.; Fornal, B.; Hecht, A. A.; Hoteling, N.; Hoffman, C.; Tabor, S. L.; Walters, W. B.; Horoi, M.; Janssens, R. V. F.; Zhu, S.; Pereira, J.; Sumikama, T.; Wang, X.

    2008-01-01

    β-decay properties of neutron-rich Ca isotopes have been obtained. Half-life values were determined for the first time for 54 Ca (86±7 ms), 55 Ca (22±2 ms), and 56 Ca (11±2 ms). The half-life of 230±60 ms deduced for 53 Ca is significantly longer than reported previously, where the decay chain 53 K → 53 Ca → 53 Sc was considered. A delayed γ ray with an energy of 247 keV was identified following β decay of 54 Ca and is proposed to depopulate the 1 1 + level in 54 Sc. The β-decay properties compare favorably with the results of shell-model calculations completed in the full pf space with the GXPF1 interaction. The half-lives of the neutron-rich Ca isotopes are also compared with gross β-decay theory. The systematic trend of the neutron-rich Ca half-lives is consistent with the presence of a subshell gap at N=32

  4. Kinetic studies of calcium in human by 41Ca tracing

    International Nuclear Information System (INIS)

    Boaretto, E.; Paul, M.; Gelbart, Z.; Johnson, R.R.; Meirav, O.; Sossi, V.; Venczel, E.; Hollos, G.; Prior, J.

    1992-01-01

    41 Ca (T 1/2 = 1 x 10 5 years) is an ideal tracer for long-term studies of calcium kinetics in biological systems for several reasons: (a) it behaves as a stable nuclide for all practical purpose due to its very long radioactive half-life; (b) its natural abundance is extremely low; (c) 41 Ca isotopic concentrations can now be measured to very high sensitivity by accelerator mass spectrometry, requiring therefore injection doses with no collateral radiation effects. Such an experiment has been initiated in a human subject by intravenous injection of a 126 ng dose of 41 Ca on July 18, 1990. The 41 Ca concentration and the 41 Ca/Ca isotopic ratios in serum and urine have been measured at five times over a 270 days period, showing a decrease in agreement with known time constants of the different calcium pools. It is planned to follow up the steady-state 41 Ca level ( 41 Ca/Ca ∼9 x 10 -12 ) over time (e.g. menstrual cycle, age)

  5. Pathways for Energization of Ca in Mercury's Exosphere

    Science.gov (United States)

    Killen, Rosemary M.

    2015-01-01

    We investigate the possible pathways to produce the extreme energy observed in the calcium exosphere of Mercury. Any mechanism must explain the facts that Ca in Mercury's exosphere is extremely hot, that it is seen almost exclusively on the dawnside of the planet, and that its content varies seasonally, not sporadically. Simple diatomic molecules or their clusters are considered, focusing on calcium oxides while acknowledging that Ca sulfides may also be the precursor molecules. We first discuss impact vaporization to justify the assumption that CaO and Ca-oxide clusters are expected from impacts on Mercury. Then we discuss processes by which the atomic Ca is energized to a 70,000 K gas. The processes considered are (1) electron-impact dissociation of CaO molecules, (2) spontaneous dissociation of Ca-bearing molecules following impact vaporization, (3) shock-induced dissociative ionization, (4) photodissociation and (5) sputtering. We conclude that electron-impact dissociation cannot produce the required abundance of Ca, and sputtering cannot reproduce the observed spatial and temporal variation that is measured. Spontaneous dissociation is unlikely to result in the high energy that is seen. Of the two remaining processes, shock induced dissociative ionization produces the required energy and comes close to producing the required abundance, but rates are highly dependent on the incoming velocity distribution of the impactors. Photodissociation probably can produce the required abundance of Ca, but simulations show that photodissociation cannot reproduce the observed spatial distribution.

  6. Vibrational spectroscopic study of poldervaartite CaCa[SiO3(OH)(OH)

    Science.gov (United States)

    Frost, Ray L.; López, Andrés; Scholz, Ricardo; Lima, Rosa Malena Fernandes

    2015-02-01

    We have studied the mineral poldervaartite CaCa[SiO3(OH)(OH)] which forms a series with its manganese analogue olmiite CaMn[SiO3(OH)](OH) using a range of techniques including scanning electron microscopy, thermogravimetric analysis, Raman and infrared spectroscopy. Chemical analysis shows the mineral is reasonably pure and contains only calcium and manganese with low amounts of Al and F. Thermogravimetric analysis proves the mineral decomposes at 485 °C with a mass loss of 7.6% compared with the theoretical mass loss of 7.7%. A strong Raman band at 852 cm-1 is assigned to the SiO stretching vibration of the SiO3(OH) units. Two Raman bands at 914 and 953 cm-1 are attributed to the antisymmetric vibrations. Intense prominent peaks observed at 3487, 3502, 3509, 3521 and 3547 cm-1 are assigned to the OH stretching vibration of the SiO3(OH) units. The observation of multiple OH bands supports the concept of the non-equivalence of the OH units. Vibrational spectroscopy enables a detailed assessment of the molecular structure of poldervaartite.

  7. Nonspatial Sequence Coding in CA1 Neurons.

    Science.gov (United States)

    Allen, Timothy A; Salz, Daniel M; McKenzie, Sam; Fortin, Norbert J

    2016-02-03

    The hippocampus is critical to the memory for sequences of events, a defining feature of episodic memory. However, the fundamental neuronal mechanisms underlying this capacity remain elusive. While considerable research indicates hippocampal neurons can represent sequences of locations, direct evidence of coding for the memory of sequential relationships among nonspatial events remains lacking. To address this important issue, we recorded neural activity in CA1 as rats performed a hippocampus-dependent sequence-memory task. Briefly, the task involves the presentation of repeated sequences of odors at a single port and requires rats to identify each item as "in sequence" or "out of sequence". We report that, while the animals' location and behavior remained constant, hippocampal activity differed depending on the temporal context of items-in this case, whether they were presented in or out of sequence. Some neurons showed this effect across items or sequence positions (general sequence cells), while others exhibited selectivity for specific conjunctions of item and sequence position information (conjunctive sequence cells) or for specific probe types (probe-specific sequence cells). We also found that the temporal context of individual trials could be accurately decoded from the activity of neuronal ensembles, that sequence coding at the single-cell and ensemble level was linked to sequence memory performance, and that slow-gamma oscillations (20-40 Hz) were more strongly modulated by temporal context and performance than theta oscillations (4-12 Hz). These findings provide compelling evidence that sequence coding extends beyond the domain of spatial trajectories and is thus a fundamental function of the hippocampus. The ability to remember the order of life events depends on the hippocampus, but the underlying neural mechanisms remain poorly understood. Here we addressed this issue by recording neural activity in hippocampal region CA1 while rats performed a

  8. Determination of standard free energies of formation of Ca3P2 and Ca2Sn at high temperatures

    Science.gov (United States)

    Min, D. J.; Sano, N.

    1988-06-01

    The standard free energies of formation of calcium phosphide and calcium stannide were determined by a chemical equilibration technique, yielding the following results: 3Ca(1) + P2(g) = Ca3P2(s) Δ G° = -653,460(±7110) + 144.01(±4.98) T (J/mol)1000 °C to 1300 °C2Ca(1) + Sn(1) = Ca2Sn(s) Δ G° = -353,970(±1670) + 79.28(±1.26) T (J/mol)1000 °C to 1300 °C 1120 °C The experimental data to express the thermodynamics for removal of phosphorus and tin from molten iron by calcium based slags by other investigators were discussed in terms of the activity co-efficients of Ca3P2 and Ca2Sn in slag melts by using the present results described above.

  9. Tumour associated antigen CA-50, CA-242 immunoradiometric assay (IRMA) in genitourinary malignancy and gastrointestinal carcinoma early diagnosis

    International Nuclear Information System (INIS)

    Chen Zhizhou.

    1992-04-01

    Tumour markers CA-50 and CA-242 were measured by immunometric assay (IRMA) to investigate their usefulness in the diagnosis of cancer of the pancreas, biliary tract, liver, breast, lung, gastrointestinal and genitourinary systems. The cutoff points, derived from studies on normal subjects and those with proven benign disease, were 20 u/ml and 12 u/ml for CA-50 and CA-242 respectively. Both markers were found to be generally useful with significant differences between malignant and non malignant disease. The highest positive rates, were found in cancers of the pancreas and gall bladder. The overall rate of false positives was low. It is concluded that measurements of CA-50 and CA-242 are useful in the detection of malignancy, particularly of the pancreas and biliary tract. 2 figs, 2 tabs

  10. Should Degree Programs in Biomedical and Health Informatics be Dedicated or Integrated? : Reflections and Recommendations after more than 40 Years of Medical Informatics Education at TU Braunschweig, including 10 Years of B.Sc. and 15 Years of M.Sc. Integrated Degree Curricula.

    Science.gov (United States)

    Haux, Reinhold; Marschollek, Michael; Wolf, Klaus-Hendrik; Zeisberg, Ute

    2017-07-01

    Education in biomedical and health informatics (BMHI) has been established in many countries throughout the world. For degree programs in BMHI we can distinguish between those that are completely stand-alone or dedicated to the discipline vs. those that are integrated within another program. After running integrated degree medical informatics programs at TU Braunschweig for 10 years at the B.Sc. and for 15 years at the M.Sc level, we (1) report about this educational approach, (2) analyze recommendations on, implementations of, and experiences with degree educational programs in BMHI worldwide, (3) summarize our lessons learned with the integrated approach at TU Braunschweig, and (4) suggest an answer to the question, whether degree programs in biomedical and health informatics should be dedicated or integrated. According to our experience at TU Braunschweig and based on our analysis of publications, there is a clear dominance of dedicated degree programs in BMHI. The specialization in medical informatics within a computer science program, as offered at TU Braunschweig, may be a good way of implementing an integrated, informatics-based approach to medical informatics, in particular if a dual degree option can be chosen. The option of curricula leading to double degrees, i.e. in this case to two separate degrees in computer science and in medical informatics might, however, be a better solution.

  11. Elementary properties of CaV1.3 Ca2+ channels expressed in mouse cochlear inner hair cells

    Science.gov (United States)

    Zampini, Valeria; Johnson, Stuart L; Franz, Christoph; Lawrence, Neil D; Münkner, Stefan; Engel, Jutta; Knipper, Marlies; Magistretti, Jacopo; Masetto, Sergio; Marcotti, Walter

    2010-01-01

    Mammalian cochlear inner hair cells (IHCs) are specialized to process developmental signals during immature stages and sound stimuli in adult animals. These signals are conveyed onto auditory afferent nerve fibres. Neurotransmitter release at IHC ribbon synapses is controlled by L-type CaV1.3 Ca2+ channels, the biophysics of which are still unknown in native mammalian cells. We have investigated the localization and elementary properties of Ca2+ channels in immature mouse IHCs under near-physiological recording conditions. CaV1.3 Ca2+ channels at the cell pre-synaptic site co-localize with about half of the total number of ribbons present in immature IHCs. These channels activated at about −70 mV, showed a relatively short first latency and weak inactivation, which would allow IHCs to generate and accurately encode spontaneous Ca2+ action potential activity characteristic of these immature cells. The CaV1.3 Ca2+ channels showed a very low open probability (about 0.15 at −20 mV: near the peak of an action potential). Comparison of elementary and macroscopic Ca2+ currents indicated that very few Ca2+ channels are associated with each docked vesicle at IHC ribbon synapses. Finally, we found that the open probability of Ca2+ channels, but not their opening time, was voltage dependent. This finding provides a possible correlation between presynaptic Ca2+ channel properties and the characteristic frequency/amplitude of EPSCs in auditory afferent fibres. PMID:19917569

  12. Izlaganje na javni uvid podataka o nekretninama i utvrđivanje prava na zemljištu u funkciji uspostave jedinstvenog registra zemljišta i prava na zemljištu u Brčko distriktu BiH : Public presentation of data on real-estate and determination of rights in function of the establishment of a single registry of real-estate and real rights to real-estate in the Brcko district of B&H

    Directory of Open Access Journals (Sweden)

    Faruk Latifović

    2014-12-01

    Full Text Available Zakon o registru zemljišta i prava na zemljištu Brčko distrikta Bosne i Hercegovine je propisao uspostavu jedinstvene evidencije nekretnina i stvarnih prava na nekretninama u Brčko distriktu Bosne i Hercegovine. Poslovi uspostave jedinstvene evidencije, u segmentu izlaganja na javni uvid podataka o nekretninama i utvrđivanje prava, su povjereni Vijeću za izlaganje podataka o nekretninama i utvrđivanje prava na zemljištu Brčko distrikta Bosne i Hercegovine. Autor se, u ovom radu, uz nezaobilazan kraći prikaz historijata javnih evidencija na nekretninama u Bosni i Hercegovini, bavi organizacijom, nadležnostima i načinom rada Vijeća za izlaganje podataka o nekretninama i utvrđivanje prava na zemljištu Brčko distrikta BIH. Nakon što bude uspješno okončan projekat izlaganje podataka o nekretninama i utvrđivanje prava na zemljištu u Brčko distriktu BiH, svi geodetsko – katastarski podaci o nepokretnostima kao i podaci o njihovom pravnom statusu će biti vođeni u Registru zemljišta i prava na zemljištu Osnovnog suda Brčko distrikta BiH. : The Law of Land Registry and Rights over Land of the Brčko District of B&H stipulated the establishment of a single registry of real-estate and real rights to real-estate in the Brčko District of B&H. Activities of the establishment of a single registry, in the segment of public presentation of data on real-estate and determination of rights, were entrusted to the Council for Presentation of Data on Real-estate and Establishment of Rights to Land of the Brčko District of B&H. In this paper, the author presents the inevitable short overview of the history of real-estate registries in Bosnia and Herzegovina and he also deals with organization, competencies and manner of work of the Council for Presentation of Data on Real-estate and Establishment of Rights to Land of the Brčko District of B&H. After a successful completion of the project of presentation of data on real-estate and

  13. RESEM-CA: Validation and testing

    Energy Technology Data Exchange (ETDEWEB)

    Pal, Vineeta; Carroll, William L.; Bourassa, Norman

    2002-09-01

    This report documents the results of an extended comparison of RESEM-CA energy and economic performance predictions with the recognized benchmark tool DOE2.1E to determine the validity and effectiveness of this tool for retrofit design and analysis. The analysis was a two part comparison of patterns of (1) monthly and annual energy consumption of a simple base-case building and controlled variations in it to explore the predictions of load components of each program, and (2) a simplified life-cycle cost analysis of the predicted effects of selected Energy Conservation Measures (ECMs). The study tries to analyze and/or explain the differences that were observed. On the whole, this validation study indicates that RESEM is a promising tool for retrofit analysis. As a result of this study some factors (incident solar radiation, outside air film coefficient, IR radiation) have been identified where there is a possibility of algorithmic improvements. These would have to be made in a way that does not sacrifice the speed of the tool, necessary for extensive parametric search of optimum ECM measures.

  14. 48Ca HETEROGENEITY IN DIFFERENTIATED METEORITES

    International Nuclear Information System (INIS)

    Chen, Hsin-Wei; Lee, Typhoon; Lee, Der-Chuen; Shen, Jason Jiun-San; Chen, Jiang-Chang

    2011-01-01

    Isotopic heterogeneities of 48 Ca have been found in numerous bulk meteorites that are correlated with 50 Ti and 54 Cr anomalies among differentiated planetary bodies, and the results suggest that a rare subset of neutron-rich Type Ia supernova (nSN Ia) was responsible for contributing these neutron-rich iron-group isotopes into the solar system (SS). The heterogeneity of these isotopes found in differentiated meteorites indicates that the isotopic compositions of the bulk SS are not uniform, and there are significant amounts of nSNe Ia dust incompletely mixed with the rest of SS materials during planetary formation. Combined with the data of now-extinct short-lived nuclide 60 Fe, which can be produced more efficiently from an nSN Ia than a Type II supernova ejecta, the observed planetary-scale isotopic heterogeneity probably reflects a late input of stellar dust grains with neutron-rich nuclear statistical equilibrium nuclides into the early SS.

  15. Asymmetries of the solar Ca II lines

    International Nuclear Information System (INIS)

    Heasley, J.N.

    1975-01-01

    A theoretical study of the influence of propagating acoustic pulses in the solar chromosphere upon the line profiles of the Ca II resonance and infrared triplet lines has been made. The major objective has been to explain the observed asymmetries seen in the cores of the H and K lines and to predict the temporal behavior of the infrared lines caused by passing acoustic or shock pulses. The velocities in the pulses, calculated from weak shock theory, have been included consistently in the non-LTE calculations. The results of the calculations show that these lines are very sensitive to perturbations in the background atmosphere caused by the pulses. Only minor changes in the line shapes result from including the velocities consistently in the line source function calculations. The qualitative changes in the line profiles vary markedly with the strength of the shock pulses. The observed differences in the K line profiles seen on the quiet Sun can be explained in terms of a spectrum of pulses with different wavelengths and initial amplitudes in the photosphere. (Auth.)

  16. Flavonoid Myricetin Modulates GABAA Receptor Activity through Activation of Ca2+ Channels and CaMK-II Pathway

    Directory of Open Access Journals (Sweden)

    Xiao Hu Zhang

    2012-01-01

    Full Text Available The flavonoid myricetin is found in several sedative herbs, for example, the St. John's Wort, but its influence on sedation and its possible mechanism of action are unknown. Using patch-clamp technique on a brain slice preparation, the present study found that myricetin promoted GABAergic activity in the neurons of hypothalamic paraventricular nucleus (PVN by increasing the decay time and frequency of the inhibitory currents mediated by GABAA receptor. This effect of myricetin was not blocked by the GABAA receptor benzodiazepine- (BZ- binding site antagonist flumazenil, but by KN-62, a specific inhibitor of the Ca2+/calmodulin-stimulated protein kinase II (CaMK-II. Patch clamp and live Ca2+ imaging studies found that myricetin could increase Ca2+ current and intracellular Ca2+ concentration, respectively, via T- and L-type Ca2+ channels in rat PVN neurons and hypothalamic primary culture neurons. Immunofluorescence staining showed increased phosphorylation of CaMK-II after myricetin incubation in primary culture of rat hypothalamic neurons, and the myricetin-induced CaMK-II phosphorylation was further confirmed by Western blotting in PC-12 cells. The present results suggest that myricetin enhances GABAA receptor activity via calcium channel/CaMK-II dependent mechanism, which is distinctively different from that of most existing BZ-binding site agonists of GABAA receptor.

  17. Ab-initio calculations of the Ruddlesden-Popper phases CaMnO{sub 3}, CaO(CaMnO{sub 3}) and CaO(CaMnO{sub 3}){sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Cardoso, C [CFC, Physics Department, University of Coimbra, 3004-516 Coimbra (Portugal); Borges, R P [Centro de Fisicada Materia Condensada - Universidade de Lisboa, Campo Grande, Ed. C8, 1749-016, Lisbon (Portugal); Gasche, T [Centro de Fisicada Materia Condensada - Universidade de Lisboa, Campo Grande, Ed. C8, 1749-016, Lisboa (Portugal); Godinho, M [Centro de Fisicada Materia Condensada - Universidade de Lisboa, Campo Grande, Ed. C8, 1749-016, Lisbon (Portugal)

    2008-01-23

    The present work reports ab-initio density functional theory calculations for the Ruddlesden-Popper phase CaO(CaMnO{sub 3}){sub n} compounds. In order to study the evolution of the properties with the number of perovskite layers, a detailed analysis of the densities of states calculated for each compound and for several magnetic configurations was performed. The effect of distortions of the crystal structure on the magnetic ground state is also analysed and the exchange constants and transition temperatures are calculated for the three compounds using a mean field model. The calculated magnetic ground state structures and magnetic moments are in good agreement with experimental results and previous calculations.

  18. Clinical analysis of the value of combined detection of serum CA199 and CA50 levels in patients with pancreatic cancer

    International Nuclear Information System (INIS)

    Zhang Zhuo; Shi Jingbin; Zheng Yongsheng

    2001-01-01

    Objective: To investigate the clinical value of combined test of serum CA 199 and CA 50 levels in the diagnosis and prognosis of patients with pancreatic cancer. Methods: Serum levels of CA 199 and CA 50 in 40 cases of pancreatic cancer, 20 cases of chronic pancreatitis and 30 normal controls were detected by radioimmunoassay. Results: Serum CA 199 and CA 50 levels in pancreatic cancer patients were significantly higher than those in normal controls and chronic pancreatitis patients (P 199 and CA 50 were detected simultaneously, the sensitivity, specificity and accuracy of diagnosis of pancreatic cancer could be improved. Serum CA 199 and CA 50 levels in cancer patients with lymph node metastasis were much higher than those without lymph node metastasis (P 199 and CA 50 detected simultaneously could improve the sensitivity and accuracy in diagnosis of pancreatic cancer. The changes of serum CA 199 and CA 50 level were helpful to predict whether metastasis or recurrence had occurred

  19. Clinical evaluation of CEA, CA19-9, CA72-4 and CA125 in gastric cancer patients with neoadjuvant chemotherapy.

    Science.gov (United States)

    Sun, Zhipeng; Zhang, Nengwei

    2014-12-29

    In the clinical practice of neoadjuvant chemotherapy, response markers are very important. We aimed o investigate whether tumor markers CEA(carcino-embryonic antigen), CA19-9(carbohydrate antigen 19-9), CA72-4(carbohydrate antigen 72-4), and CA125(carbohydrate antigen 125) can be used to evaluate the response to neoadjuvant chemotherapy, and to evaluate the diagnosis and prognosis value of four tumor markers in the patients of gastric cancer. A retrospective review was performed of 184 gastric cancer patients who underwent a 5-Fu, leucovorin, and oxaliplatin (FOLFOX) neoadjuvant chemotherapy regimen, followed by surgical treatment. Blood samples for CEA, CA19-9, CA72-4, and CA125 levels were taken from patients upon admission to the hospital and after neoadjuvant chemotherapy. Statistical analysis was performed to identify the clinical value of these tumor markers in predicting the survival and the response to neoadjuvant chemotherapy. Median overall survival times of pretreatment CA19-9-positive and CA72-4-positive patients (14.0 +/-2.8 months and 14.8 +/-4.0 months, respectively) were significantly less than negative patients (32.5 +/-8.9 months and 34.0 +/-10.1 months, respectively) (P = 0.000 and P = 0.002, respectively). Pretreatment status of CA19-9 and CA72-4 were independent prognostic factors in gastric cancer patients (P = 0.029 and P = 0.008, respectively). Pretreatment CEA >50 ng/ml had a positive prediction value for clinical disease progression after neoadjuvant chemotherapy according to the ROC curve (AUC: 0.694, 95% CI: 0.517 to 0.871, P = 0.017). The decrease of tumor markers CEA, CA72-4, and CA125 was significant after neoadjuvant chemotherapy (P = 0.030, P = 0.010, and P = 0.009, respectively), especially in patients with disease control (including complete, partial clinical response, and stable disease) (P = 0.012, P = 0.020, and P = 0.025, respectively). A decrease in CA72-4 by more than 70% had

  20. The diagnostic value of serum tumor markers CEA, CA19-9, CA125, CA15-3, and TPS in metastatic breast cancer.

    Science.gov (United States)

    Wang, Weigang; Xu, Xiaoqin; Tian, Baoguo; Wang, Yan; Du, Lili; Sun, Ting; Shi, Yanchun; Zhao, Xianwen; Jing, Jiexian

    2017-07-01

    This study aims to understand the diagnostic value of serum tumor markers carcinoembryonic antigen (CEA), cancer antigen 19-9 (CA19-9), cancer antigen 125 (CA125), cancer antigen 15-3 (CA15-3), and tissue polypeptide-specific antigen (TPS) in metastatic breast cancer (MBC). A total of 164 metastatic breast cancer patients in Shanxi Cancer Hospital were recruited between February 2016 and July 2016. 200 breast cancer patients without metastasis in the same period were randomly selected as the control group. The general characteristics, immunohistochemical, and pathological results were investigated between the two groups, and tumor markers were determined. There were statistical differences in the concentration and the positive rates of CEA, CA19-9, CA125, CA15-3, and TPS between the MBC and control group (Ptumor marker at 56.7% and 97.0%, respectively. In addition, two tumor markers were used for the diagnosis of MBC and the CEA and TPS combination had the highest diagnostic sensitivity with 78.7%, while the CA15-3 and CA125 combination had the highest specificity of 91.5%. Analysis of tumor markers of 164 MBC found that there were statistical differences in the positive rates of CEA and CA15-3 between bone metastases and other metastases (χ 2 =6.00, P=0.014; χ 2 =7.32, P=0.007, respectively). The sensitivity and specificity values of the CEA and CA15-3 combination in the diagnosis of bone metastases were 77.1% and 45.8%, respectively. The positive rate of TPS in the lung metastases group was lower than in other metastases (χ 2 =8.06, P=0.005).There were significant differences in the positive rates of CA15-3 and TPS between liver metastases and other metastases (χ 2 =15.42, Ptumor markers have varying diagnostic value. Copyright © 2017 Elsevier B.V. All rights reserved.

  1. Preparation of 45Ca(HDEHP)n and (CaH1502)2 samples for liquid scintillation counting, compared to 45caCl2 results

    International Nuclear Information System (INIS)

    Rodriguez, L.; Arcos, J. M. los; Grau Malonda, A.

    1994-01-01

    A procedure for preparation of liquid scintillation counting organic samples of the Di-2-ethylhexyl phosphate calcium complex and the 2-ethylhexanoate calcium salt, labelled with 45Ca, is described. The chemical quench, the counting stability and spectral evolution of both compounds is studied in six scintillators,Toluene-alcohol, Dioxane-naphtalene, Hi safe II, Ultimate-Gold and Instagel, and compared to results obtained from a commercial solution of 4 5CaCl2. (Author) 7 refs

  2. Studies of highly ionized atoms using internal conversion: 197Au, 57Fe; electric monopole transitions in 40Ca, 42Ca, and 44Ca

    International Nuclear Information System (INIS)

    Ulrickson, M.A.

    1975-01-01

    By using heavy-ion reactions, highly ionized electronic states of atoms may be produced. The interaction between excited nuclear levels and the surrounding atomic electrons via internal conversion allows the nucleus to be used as a probe of the electronic structure of the highly ionized atoms. Studies of such atoms were undertaken for strongly internally converted nuclear levels in 197 Au and 57 Fe. The nuclear levels were Coulomb excited by using 16 O and 32 S beams. Simultaneous measurement of the lifetime of the 77-keV state of 197 Au in both neutral gold atoms and gold atoms with mean charge +10 resulted in a measured change in the internal conversion coefficient of Δalpha/alpha equals - 1.7 +- 3.0)10 -3 . This result is consistent with calculations using a Hartree-Fock--Slater program. Measurements of the electric monopole strengths for 0 + → 0 + transitions were undertaken to determine the amount of core-deformation in calcium nuclei. The E0 strengths for the decays of the 0 + states at 5.21 MeV in 40 C, 1.84 MeV in 42 Ca, and 1.88 MeV in 44 Ca were observed. The branching ratios for the subsequent E0 pair decays were measured by observing the coincident annihilation radiation from the e + member of the pair in coincidence with protons feeding the state in the cases of 42 Ca and 44 Ca, and by observing the actual coincident e + --e - pair together with protons feeding the state in the case of 40 Ca. The resulting E0 strengths (rho less than or equal to 0.06, rho = 0.34 +- 0.03, rho = 0.30 +- 0.10 for 40 Ca, 42 Ca, and 44 Ca respectively) agree with theoretical descriptions

  3. {sup 41}Ca measurements at the Zurich AMS facility

    Energy Technology Data Exchange (ETDEWEB)

    Schnabel, C.; Gartenmann, P.; Suter, M. [Eidgenoessische Technische Hochschule, Zurich (Switzerland); Synal, H.A. [Paul Scherrer Inst. (PSI), Villigen (Switzerland); Gloris, M.; Leya, I.; Michel, R. [Hannover Univ. (Germany); Herpers, U. [Koeln Univ. (Germany)

    1997-09-01

    Proton-induced production cross sections for {sup 41}Ca from Fe and Ni determined using 6 MV tandem accelerator are presented. The calibration of two secondary standard materials to a standard material of {sup 41}Ca concentration determined by PTB (Braunschweig, Germany) has been carried out. (author) 2 figs., 3 refs.

  4. Isolation and characterization of higher metallofullerenes Ca@ C 92 ...

    Indian Academy of Sciences (India)

    ... technique without recycling equipment. It is notable that higher metallofullerenes containing alkaline earth metal ions could be isolated although their relative yields are extremely low. Ca@C92 and Ca@C94 are also confirmed by LD-TOF mass spectrometry and characterrized by UV-Vis-NIR absorption measurements.

  5. Characterization of Binding Epitopes of CA125 Monoclonal Antibodies

    DEFF Research Database (Denmark)

    Marcos-Silva, Lara; Narimatsu, Yoshiki; Halim, Adnan

    2014-01-01

    The most used cancer serum biomarker is the CA125 immunoassay for ovarian cancer that detects the mucin glycoprotein MUC16. Several monoclonal antibodies (mAbs) including OC125 and M11 are used in CA125 assays. However, despite considerable efforts, our knowledge of the molecular characteristics ...

  6. Isolation and characterization of higher metallofullerenes Ca@C92 ...

    Indian Academy of Sciences (India)

    Administrator

    allofullerenes containing alkaline earth metal ions could be isolated although their relative yields are extremely low. Ca@C92 and Ca@C94 are also ... est cage size for lanthanide and alkaline earth higher metallofullerenes isolated and ... suming stage is to remove abundant hollow small cage fullerenes and enrich higher ...

  7. Facile combustion synthesis of novel CaZrO 3

    Indian Academy of Sciences (India)

    Abstract. A facile sol–gel combustion route was reported for the direct preparation of CaZrO3:Eu3+ and CaZrO3:Eu3+, Gd3+. The obtained deposits were characterized by XRD, TGA-DSC, SEM, EDS, PL measurements and microscope fluorescence. When the Gd3+ ions were introduced in this compound, the emissions of ...

  8. SNL/CA Environmental Management System Program Manual.

    Energy Technology Data Exchange (ETDEWEB)

    Larsen, Barbara L.

    2005-09-01

    The Sandia National Laboratories, California (SNL/CA) Environmental Management System (EMS) Program Manual documents the elements of the site EMS Program. The SNL/CA EMS Program was developed in accordance with Department of Energy (DOE) Order 450.1 and incorporates the elements of the International Standard on Environmental Management Systems, ISO 14001.

  9. Isolation and characterization of higher metallofullerenes Ca@C92 ...

    Indian Academy of Sciences (India)

    Administrator

    allofullerenes containing alkaline earth metal ions could be isolated although their relative yields are extremely low. Ca@C92 and Ca@C94 ... laser vapourization of a graphite–metal composite in a helium filled tube oven at high ... robenzene extraction, chlorobenzene and benzene mo- bile phase for isolation of fullerenes ...

  10. Unanimous Model for Describing the Fast Bioluminescence Kinetics of Ca

    NARCIS (Netherlands)

    Eremeeva, Elena V.; Bartsev, Sergey I.; Berkel, van Willem J.H.; Vysotski, Eugene S.

    2017-01-01

    Upon binding their metal ion cofactors, Ca2+-regulated photoproteins display a rapid increase of light signal, which reaches its peak within milliseconds. In the present study, we investigate bioluminescence kinetics of the entire photoprotein family. All five recombinant hydromedusan Ca2+-regulated

  11. 76 FR 45180 - Modification of Class E Airspace; Alturas, CA

    Science.gov (United States)

    2011-07-28

    ...-0403; Airspace Docket No. 11-AWP-3] Modification of Class E Airspace; Alturas, CA AGENCY: Federal... Alturas Municipal Airport, Alturas, CA. Controlled airspace is necessary to accommodate aircraft using... Alturas Municipal Airport. This improves the safety and management of Instrument Flight Rules (IFR...

  12. Characterization of the Ca2+ Channels Involved in the Progesterone ...

    African Journals Online (AJOL)

    There is evidence that intracellular Ca2+ concentration plays significant roles in sperm function such as motility and acrosome reaction. Many calcium channels have been identified in the plasma membrane of sperm. Progesterone (P4) stimulates Ca2+ influx and acrosome reaction in human spermatozoa. The effects of ...

  13. PIK3CA mutant tumors depend on oxoglutarate dehydrogenase

    Science.gov (United States)

    Ilic, Nina; Birsoy, Kıvanç; Aguirre, Andrew J.; Kory, Nora; Pacold, Michael E.; Singh, Shambhavi; Moody, Susan E.; DeAngelo, Joseph D.; Spardy, Nicole A.; Freinkman, Elizaveta; Weir, Barbara A.; Cowley, Glenn S.; Root, David E.; Asara, John M.; Vazquez, Francisca; Widlund, Hans R.; Sabatini, David M.; Hahn, William C.

    2017-01-01

    Oncogenic PIK3CA mutations are found in a significant fraction of human cancers, but therapeutic inhibition of PI3K has only shown limited success in clinical trials. To understand how mutant PIK3CA contributes to cancer cell proliferation, we used genome scale loss-of-function screening in a large number of genomically annotated cancer cell lines. As expected, we found that PIK3CA mutant cancer cells require PIK3CA but also require the expression of the TCA cycle enzyme 2-oxoglutarate dehydrogenase (OGDH). To understand the relationship between oncogenic PIK3CA and OGDH function, we interrogated metabolic requirements and found an increased reliance on glucose metabolism to sustain PIK3CA mutant cell proliferation. Functional metabolic studies revealed that OGDH suppression increased levels of the metabolite 2-oxoglutarate (2OG). We found that this increase in 2OG levels, either by OGDH suppression or exogenous 2OG treatment, resulted in aspartate depletion that was specifically manifested as auxotrophy within PIK3CA mutant cells. Reduced levels of aspartate deregulated the malate–aspartate shuttle, which is important for cytoplasmic NAD+ regeneration that sustains rapid glucose breakdown through glycolysis. Consequently, because PIK3CA mutant cells exhibit a profound reliance on glucose metabolism, malate–aspartate shuttle deregulation leads to a specific proliferative block due to the inability to maintain NAD+/NADH homeostasis. Together these observations define a precise metabolic vulnerability imposed by a recurrently mutated oncogene. PMID:28396387

  14. Purification and biochemical characterization of a Ca2+- ...

    African Journals Online (AJOL)

    On the other hand, Ca+2 and EDTA (1 to 5 mM) did not significantly affect the enzyme activity. The main substrate for the enzyme was found to be starch but it could also hydrolyze raw starch, dextrin, γ-cyclodextrin and pullulan. Key words: Ca2+-independent, Bacillus sp, thermostable α-amylases, low pH profile, enzyme, ...

  15. Purification and characterization of a Ca -dependent/calmodulin ...

    Indian Academy of Sciences (India)

    Unknown

    mitrella patens and Funaria hygrometrica, calcium acts as an intracellular messenger in cytokinin-induced vege- tative bud formation (Schumaker and Gizinski 1995). Calcium-free medium (buffered with EGTA), the extra- cellular Ca2+ antagonist lanthanum (La3+) and the Ca2+ channel inhibitors, D600 and verapamil, ...

  16. Synthesis and microwave dielectric properties of Ca substituted ...

    Indian Academy of Sciences (India)

    Keywords. Patch antenna; theoretical density; phase; ceramics. Abstract. Microwave dielectric ceramics in Sr1-CaLa4Ti4.93Zr0.07O17 (0 ≤ ≤ 0.5) composition series were processed via a solid-state sintering rout. X-ray diffraction revealed single phase ceramics. Ca substitutions for Sr tuned f towards zero with ...

  17. Hierarchic stochastic modelling applied to intracellular Ca(2+ signals.

    Directory of Open Access Journals (Sweden)

    Gregor Moenke

    Full Text Available Important biological processes like cell signalling and gene expression have noisy components and are very complex at the same time. Mathematical analysis of such systems has often been limited to the study of isolated subsystems, or approximations are used that are difficult to justify. Here we extend a recently published method (Thurley and Falcke, PNAS 2011 which is formulated in observable system configurations instead of molecular transitions. This reduces the number of system states by several orders of magnitude and avoids fitting of kinetic parameters. The method is applied to Ca(2+ signalling. Ca(2+ is a ubiquitous second messenger transmitting information by stochastic sequences of concentration spikes, which arise by coupling of subcellular Ca(2+ release events (puffs. We derive analytical expressions for a mechanistic Ca(2+ model, based on recent data from live cell imaging, and calculate Ca(2+ spike statistics in dependence on cellular parameters like stimulus strength or number of Ca(2+ channels. The new approach substantiates a generic Ca(2+ model, which is a very convenient way to simulate Ca(2+ spike sequences with correct spiking statistics.

  18. Maternal serum CA-125 level is elevated in severe preeclampsia.

    Science.gov (United States)

    Karaman, Erbil; Karaman, Yasemin; Alkış, İsmet; Han, Agahan; Yıldırım, Gökhan; Ark, Hasan Cemal

    2014-01-01

    The aim of this study was to determine the relationship between serum concentrations of cancer antigen-125 (CA-125) and pre-eclampsia severity. We evaluated 91 females with a singleton pregnancy. Serum CA-125 levels were measured in subjects with severe pre-eclampsia (n=34) and those with mild pre-eclampsia (n=24). Females with healthy pregnancies (n=31) served as the control group. The three study groups were statistically similar in terms of maternal age, gestational age, and body mass index. The CA-125 level was significantly higher in the severe pre-eclampsia group than that in the mild pre-eclampsia and control groups (pCA-125 levels between the mild pre-eclampsia and control groups was observed. CA-125 level was positively correlated with proteinuria (r=0.489, p=0.000), systolic blood pressure (r=0.503, p=0.018), and diastolic blood pressure (r=0.532, p=0.000). In contrast, CA-125 was negatively correlated with birth weight (r=0.266, p=0.012) and gestational age at birth (r=0.250, p=0.018). CA-125 level increased in severe pre-eclampsia, which reflected abnormal trophoblastic invasion and chronic inflammation. Elevated levels of CA-125 in pre-eclamptic patients may be a marker of the disease severity. Copyright © 2013 International Society for the Study of Hypertension in Pregnancy. Published by Elsevier B.V. All rights reserved.

  19. CA1 contributes to microcalcification and tumourigenesis in breast cancer

    International Nuclear Information System (INIS)

    Zheng, Yabing; Xu, Bing; Zhao, Yan; Gu, He; Li, Chang; Wang, Yao; Chang, Xiaotian

    2015-01-01

    Although mammary microcalcification is frequently observed and has been associated with poor survival in patients with breast cancer, the genesis of calcification remains unclear. Carbonic anhydrase I (CA1) has been shown to promote calcification by catalysing the hydration of CO 2 . This study aimed to determine whether CA1 was correlated with microcalcification and with other processes that are involved in breast cancer tumourigenesis. CA1 expression in breast cancer tissues and blood samples was detected using western blotting, real-time PCR, immunohistochemistry and ELISA. Calcification was induced in the cultured 4T1 cell line originating from mouse breast tumours, using ascorbic acid and β-glycerophosphate. Acetazolamide, a chemical inhibitor of CA1, was also added to the culture to determine the role of CA1 in calcification. The MCF-7 human breast cancer cell line was treated with anti-CA1 siRNA and was assessed using a CCK-8 cell proliferation assay, an annexin V cell apoptosis assay, transwell migration assay and a human breast cancer PCR array. The tag SNP rs725605, which is located in the CA1 locus, was genotyped using TaqMan® genotyping. Increased CA1 expression was detected in samples of breast carcinoma tissues and blood obtained from patients with breast cancer. A total of 15.3 % of these blood samples exhibited a 2.1-fold or higher level of CA1 expression, compared to the average level of CA1 expression in samples from healthy controls. Following the induction of calcification of 4T1 cells, both the number of calcium-rich deposits and the expression of CA1 increased, whereas the calcification and CA1 expression were significantly supressed in the presence of acetazolamide. Increased migration and apoptosis were observed in MCF-7 cells that were treated with anti-CA1 siRNA. The PCR array detected up-regulation of the androgen receptor (AR) and down-regulation of X-box binding protein 1 (XBP1) in the treated MCF-7 cells. Significant differences in

  20. CA1 contributes to microcalcification and tumourigenesis in breast cancer.

    Science.gov (United States)

    Zheng, Yabing; Xu, Bing; Zhao, Yan; Gu, He; Li, Chang; Wang, Yao; Chang, Xiaotian

    2015-10-12

    Although mammary microcalcification is frequently observed and has been associated with poor survival in patients with breast cancer, the genesis of calcification remains unclear. Carbonic anhydrase I (CA1) has been shown to promote calcification by catalysing the hydration of CO2. This study aimed to determine whether CA1 was correlated with microcalcification and with other processes that are involved in breast cancer tumourigenesis. CA1 expression in breast cancer tissues and blood samples was detected using western blotting, real-time PCR, immunohistochemistry and ELISA. Calcification was induced in the cultured 4T1 cell line originating from mouse breast tumours, using ascorbic acid and β-glycerophosphate. Acetazolamide, a chemical inhibitor of CA1, was also added to the culture to determine the role of CA1 in calcification. The MCF-7 human breast cancer cell line was treated with anti-CA1 siRNA and was assessed using a CCK-8 cell proliferation assay, an annexin V cell apoptosis assay, transwell migration assay and a human breast cancer PCR array. The tag SNP rs725605, which is located in the CA1 locus, was genotyped using TaqMan® genotyping. Increased CA1 expression was detected in samples of breast carcinoma tissues and blood obtained from patients with breast cancer. A total of 15.3 % of these blood samples exhibited a 2.1-fold or higher level of CA1 expression, compared to the average level of CA1 expression in samples from healthy controls. Following the induction of calcification of 4T1 cells, both the number of calcium-rich deposits and the expression of CA1 increased, whereas the calcification and CA1 expression were significantly supressed in the presence of acetazolamide. Increased migration and apoptosis were observed in MCF-7 cells that were treated with anti-CA1 siRNA. The PCR array detected up-regulation of the androgen receptor (AR) and down-regulation of X-box binding protein 1 (XBP1) in the treated MCF-7 cells. Significant differences in

  1. Quantifying Ca2+ release and inactivation of Ca2+ release in fast- and slow-twitch muscles

    Science.gov (United States)

    Barclay, C J

    2012-01-01

    The aims of this study were to quantify the Ca2+ release underlying twitch contractions of mammalian fast- and slow-twitch muscle and to comprehensively describe the transient inactivation of Ca2+ release following a stimulus. Experiments were performed using bundles of fibres from mouse extensor digitorum longus (EDL) and soleus muscles. Ca2+ release was quantified from the amount of ATP used to remove Ca2+ from the myoplasm following stimulation. ATP turnover by crossbridges was blocked pharmacologically (N-benzyl-p-toluenesulphonamide for EDL, blebbistatin for soleus) and muscle heat production was used as an index of Ca2+ pump ATP turnover. At 20°C, Ca2+ release in response to a single stimulus was 34 and 84 μmol (kg muscle)−1 for soleus and EDL, respectively, and increased with temperature (30°C: soleus, 61 μmol kg−1; EDL, 168 μmol kg−1). Delivery of another stimulus within 100 ms of the first produced a smaller Ca2+ release. The maximum magnitude of the decrease in Ca2+ release was greater in EDL than soleus. Ca2+ release recovered with an exponential time course which was faster in EDL (mean time constant at 20°C, 32.1 ms) than soleus (65.6 ms) and faster at 30°C than at 20°C. The amounts of Ca2+ released and crossbridge cycles performed are consistent with a scheme in which Ca2+ binding to troponin-C allowed an average of ∼1.7 crossbridge cycles in the two muscles. PMID:23027818

  2. Spin decomposition of the responses of 44Ca and 48Ca to 300 MeV protons

    International Nuclear Information System (INIS)

    Baker, F.T.; Bimbot, L.; Fergerson, R.W.; Glashausser, C.; Green, A.; Haeusser, O.; Hicks, K.; Jones, K.; Miller, C.A.; Vetterli, M.; Abegg, R.; Beatty, D.; Bonin, B.; Castel, B.; Chen, X.Y.; Cupps, V.; Djalali, C.; Henderson, R.; Jackson, K.P.; Jeppesen, R.; Nakayama, K.; Nanda, S.K.; Sawafta, R.; Yen, S.; Institut de Physique Nucleaire, F-91406 Orsay, France; Rutgers University, Piscataway, New Jersey 08854; Simon Fraser University, Burnaby, Canada V5A1S6; TRIUMF, 4004 Westbrook Mall, Vancouver, Canada V6T2A3; Los Alamos National Laboratory, Los Alamos, New Mexico 87544; Centre d'Etudes Nucleaires de Saclay, 91191 Gif sur Yvette, CEDEX, France; Queen's University, Kingston, Canada K7L3N6; University of Colorado, Boulder, Colorado 80309; University of South Carolina, Columbia, South Carolina 29208; Continuous Electron Beam Accelerator Facility, 12000 Jefferson Ave., Newport News, Virginia 23606; University of Alberta, Edmonton, Canada T6G2J1)

    1991-01-01

    Angular distributions of the double-differential cross section d 2 σ/dΩ dE(σ) and the spin-flip probability S nn have been measured for inclusive proton inelastic scattering from 44 Ca at 290 MeV and from 48 Ca at 318 MeV. Excitation energies up to about 50 MeV for 44 Ca and 40 MeV for 48 Ca have been investigated over the laboratory angular ranges of 3 degree to 12 degree for 44 Ca and 3 degree to 9 degree for 48 Ca. Multipole decompositions of angular distributions of both the spin-flip cross section σS nn and the estimated cross section for ΔS=0 transitions have been performed. Distributions of strengths were deduced for ΔL=1, ΔS=0 (the giant dipole), ΔL=2, ΔS=0 (the giant quadrupole), ΔL=0, ΔS=1 (the magnetic dipole), ΔL=1, ΔS=1 (the spin dipole), and ΔL=2, ΔS=1 (the spin quadrupole). The ΔS=0 summed strengths for 44 Ca are lower than for 40 Ca and 48 Ca. The spin-dipole summed strengths are found to be approximately independent of A. For 48 Ca, essentially all M1 strength observed was in the 10.23 MeV 1 + state; for 44 Ca, M1 strength was observed to be fragmented over a range of 7 to 18 Mev

  3. Microstructure, In Vitro Corrosion Behavior and Cytotoxicity of Biodegradable Mg-Ca-Zn and Mg-Ca-Zn-Bi Alloys

    Science.gov (United States)

    Bakhsheshi-Rad, H. R.; Hamzah, E.; Tok, H. Y.; Kasiri-Asgarani, M.; Jabbarzare, S.; Medraj, M.

    2017-02-01

    The effects of bismuth (Bi) addition on the microstructure and corrosion behavior of the Mg-Ca-Zn-Bi alloys were evaluated using electron microscopy, electrochemical test and electrochemical impedance spectroscopy. Microstructural observations showed that Mg-1.2Ca-1Zn- xBi ( x = 0.5, 1.5, 3 wt.%) are composed of Mg2Ca, Ca2Mg6Zn3 and Mg3Bi2 phases while a new phase Mg2Bi2Ca appeared after the addition of 5 and 12 wt.% Bi to the Mg-1.2Ca-1Zn alloy. Furthermore, the additions of 0.5 wt.% Bi to the Mg-1.2Ca-1Zn alloy slightly improved the corrosion behavior of the alloy, while further increase in Bi amount from 1.5 to 12 wt.% has a deleterious effect on the corrosion behavior of the ternary Mg-1.2Ca-1Zn alloy which is driven by galvanic coupling effect. Cytotoxicity tests indicate that the Mg-1.2Ca-1Zn presents higher cell viability compared to Mg-1.2Ca-1Zn-0.5Bi alloy. In addition, the cell viability of both alloys increased with increasing incubation time while diluting the extracts to 50% and 10% improved the cell viabilities. The present results suggest that the Mg-1.2Ca-1Zn-0.5Bi can be interesting candidate for the development of degradable biomaterials and it is worthwhile for further investigation in an in vivo environment.

  4. Assessment of diagnostic value of various tumors markers (CEA, CA199, CA50) for colorectal neoplasm with logistic regression and ROC curve

    International Nuclear Information System (INIS)

    Gu Ping; Huang Gang; Han Yuan

    2007-01-01

    Objective: To assess the diagnostic value of CEA, CA199 and CA50 for colorectal neoplasm by logistic regression and ROC curve. Methods: Serum CEA (with CLIA), CA199 (with ECLIA) and CA50 (with IRMA) levels were measured in 75 patients with colorectal cancer, 35 patients with benign colorectal disorders and 49 controls. The area under the ROC curve (AUC)s of CEA, CA199, CA50 from logistic regression results were compared. Results: In the cancer-benign disorder group, the AUC of CA50 was larger than the AUC of CA199. AUC of combined CEA, CA50 was largest: not only larger than any AUC of CEA, CA50, CA199 alone but also larger than the AUC of the combined three markers (0.875 vs 0.604). In cancer-control group, the AUC of combination of CEA, CA199 and CA50 was larger than any AUC of CEA, CA199 or CA50 alone. Both in the cancer-benign disorder group or cancer-control group, the AUC of CEA was larger than the AUC of CA199 or CA50. Conclusion: CEA is of definite value in the diagnosis of colorectal cancer. For differential diagnosis, the combination of CEA and CA50 can give more information, while the combination of three tumor markers is less helpful. As an advanced statistical method, logistic regression can improve the diagnostic sensitivity and specificity. (authors)

  5. Ca2+-dependent K+ Channels in Exocrine Salivary Glands

    Science.gov (United States)

    Catalán, Marcelo A.; Peña-Munzenmayer, Gaspar; Melvin, James E.

    2014-01-01

    In the last 15 years, remarkable progress has been realized in identifying the genes that encode the ion-transporting proteins involved in exocrine gland function, including salivary glands. Among these proteins, Ca2+-dependent K+ channels take part in key functions including membrane potential regulation, fluid movement and K+ secretion in exocrine glands. Two K+ channels have been identified in exocrine salivary glands: 1) a Ca2+-activated K+ channel of intermediate single channel conductance encoded by the KCNN4 gene; and, 2) a voltage- and Ca2+-dependent K+ channel of large single channel conductance encoded by the KCNMA1 gene. This review focuses on the physiological roles of Ca2+-dependent K+ channels in exocrine salivary glands. We also discuss interesting recent findings on the regulation of Ca2+-dependent K+ channels by protein-protein interactions that may significantly impact exocrine gland physiology. PMID:24559652

  6. Proton resonance spectroscopy in 40Ca

    International Nuclear Information System (INIS)

    Warthen, B.J.

    1987-01-01

    The differential cross sections for the 39 K(p,p o ) 39 K and 39 K-(p,α o ) 36 Ar reactions have been measured for E p = 1.90 to 4.02 MeV at laboratory angles θ = 90 degree, 108 degree, 150 degree and 165 degree. Data were taken with the Triangle Universities Nuclear Laboratory (TUNL) KN Van de Graaff accelerator and the associated high resolution system. The targets consisted of 1-2 μg/cm 2 of potassium carbonate (K 2 CO 3 ), enriched to 99.97% 39 K, evaporated onto gold coated carbon backings. Excitation functions were measured in proton energy steps varying from 100 to 400 3V. The energy region studied corresponds to an excitation energy range in the 40 Ca nucleus of E x = 10.2 to 12.3 MeV. A multi-level multi-channel R-matrix based computer code was used to fit the experimental excitation functions. Resonance parameters obtained include resonance energy, spin, parity, partial widths, and channel spin and orbital angular momentum mixing ratios. Of the 248 resonances observed in the proton channel, 148 were also observed in the alpha channel. A fit to the observed level density yielded a nuclear temperature of 1.5 MeV. The data were compared with predictions of statistical theories of energy levels for both level spacing and reduced width distributions. The alpha reduced widths agree with the Porter-Thomas distribution and suggest that only 5-10% of the states with alpha widths were not observed. The summed strength in each of the alpha channels represents a significant fraction of the Wigner limit for these channels. The proton channels, on the other hand, generally have much smaller fractions. The two proton s-wave strength functions are equal and thus show no evidence for spin-exchange forces in the nucleon-nucleus interaction

  7. Modelling Ca2+ bound Troponin in Excitation Contraction Coupling

    Directory of Open Access Journals (Sweden)

    Henry G. Zot

    2016-09-01

    Full Text Available To explain disparate decay rates of cytosolic Ca2+ and structural changes in the thin filaments during a twitch, we model the time course of Ca2+ bound troponin (Tn resulting from the free Ca2+ transient of fast skeletal muscle. In fibers stretched beyond overlap, the decay of Ca2+ as measured by a change in fluo 3 fluorescence is significantly slower than the intensity decay of the meridional 1/38.5 nm-1 reflection of Tn; this is not simply explained by considering only the Ca2+ binding properties of Tn alone (Matsuo, T., Iwamoto, H., and Yagi, N. (2010. Biophys. J. 99, 193-200. We apply a comprehensive model that includes the known Ca2+ binding properties of Tn in the context of the thin filament with and without cycling crossbridges. Calculations based on the model predict that the transient of Ca2+ bound Tn correlates with either the fluo 3 time course in muscle with overlapping thin and thick filaments or the intensity of the meridional 1/38.5 nm-1 reflection in overstretched muscle. Hence, cycling crossbridges delay the dissociation of Ca2+ from Tn. Correlation with the fluo 3 fluorescence change is not causal given that the transient of Ca2+ bound Tn depends on sarcomere length, whereas the fluo-3 fluorescence change does not. Transient positions of tropomyosin calculated from the time course of Ca2+ bound Tn are in reasonable agreement with the transient of measured perturbations of the Tn repeat in overlap and non-overlap muscle preparations.

  8. Ca(2+)-dependence of conoid extrusion in Toxoplasma gondii tachyzoites.

    Science.gov (United States)

    Mondragon, R; Frixione, E

    1996-01-01

    The role of Ca2+ in conoid extrusion was investigated in isolated Toxoplasma gondii tachyzoites by treatment with Ca(2+)-ionophores, Ca(2+)-chelating agents and an inhibitor of the Ca(2+)-ATPase at the endoplasmic reticulum. The results were evaluated by light phase-contrast microscopy and electron microscopy. Ionomycin (0.5-1 microM) caused an immediate and sustained extrusion of the conoid in up to 80% of the tachyzoites, depending on the concentrations of ionophore and Ca2+ in the medium. However, over 50% of the tachyzoites extruded the conoid when treated with ionomycin in Ca(2+)-free saline complemented with EGTA. The effect of ionomycin was reversible and could be induced a second time in about half of the responsive population. Similar results were obtained with A23187. Conoid extrusion induced by ionomycin in Ca(2+)-free medium was almost completely abolished when the tachyzoites were previously loaded with a permeable compound known to chelate intracellular Ca2+ (BAPTA/AM; 25 microM). On the other hand, exposure of tachyzoites to the Ca(2+)-ATPase inhibitor thapsigargin (0.5-1 microM) produced significant extrusion of the conoid. Tachyzoites loaded with BAPTA/AM as well as those treated with ionomycin, i.e. with conoids paralyzed in opposite positions, had a diminished capacity to invade cultured epithelial cells. A substantial reduction in the response to stimulation by ionomycin was found also in parasites treated with cytochalasin-D, a drug that depolymerizes actin-filaments. The results suggest that Ca(2+)-release from internal stores may act as a key signal to activate a mechanism of conoid extrusion probably mediated, at least in part, by actin-filaments.

  9. Up-regulation of Ca2+/CaMKII/CREB signaling in salicylate-induced tinnitus in rats.

    Science.gov (United States)

    Zhao, Jiuhan; Wang, Biao; Wang, Xiaohong; Shang, Xiuli

    2018-02-09

    The purpose of the study was to investigate the changes of Ca 2+ /calmodulin-dependent protein kinases II (CaMKII)/cAMP response element-binding protein (CREB) signaling pathway in a rat tinnitus model. Eighteen Wistar rats were randomly divided into three groups: normal control (NC), normal saline (NS), and tinnitus model (TM) groups. Tinnitus model was induced by intraperitoneal injection of salicylate. The concentration of intracellular calcium level in auditory cortex cells was determined using Fura-2 acetoxymethyl ester (Fura-2 AM) method with fluorospectrophotometer. Expressions of calmodulin (CaM), N-methyl-D-aspartate receptor 2B subunit (NR2B), calcium-calmodulin kinase II (CaMKII), and cAMP response element-binding protein (CREB) were detected with Western blot. Tinnitus model was successfully established by the intraperitoneal administration of salicylate in rats. Compared with rats in NC and NS groups, salicylate administration significantly elevated CaM, NR2B, phospho-CaMKII and phospho-CREB expression in auditory cortex from tinnitus model group (p salicylate administration causes tinnitus symptoms and elevates Ca 2+ /CaMKII/CREB signaling pathway in auditory cortex cells. Our study likely provides a new understanding of the development of tinnitus.

  10. 45Ca2+movements induced by Ca2+chloride in isolated rat aorta under K+-free conditions

    NARCIS (Netherlands)

    Wermelskirchen, D.; Nebel, U.; Wirth, A.; Wilffert, B.

    1991-01-01

    Increasing the extracellular Ca2+concentration induced a dihydropyridine-insensitive contraction in the isolated rat aorta bathed in K+-free solution. To obtained further insight into the mechanisms of this contraction45Ca2+uptake measurements were carried out with isolated rat aorta. Increasing the

  11. CA-45(2+) MOVEMENTS INDUCED BY CA2+ CHLORIDE IN ISOLATED RAT AORTA UNDER K+-FREE CONDITIONS

    NARCIS (Netherlands)

    WERMELSKIRCHEN, D; NEBEL, U; WIRTH, A; WILFFERT, B

    1991-01-01

    Increasing the extracellular Ca2+ concentration induced a dihydropyridine-insensitive contraction in the isolated rat aorta bathed in K+-free solution. To obtain further insight into the mechanism of this contraction Ca-45(2+) uptake measurements were carried out with isolated rat aorta. Increasing

  12. Acrosome Reaction and Ca2+ Imaging in Single Human Spermatozoa: New Regulatory Roles of [Ca2+]i1

    Science.gov (United States)

    Sánchez-Cárdenas, Claudia; Servín-Vences, Martha Rocio; José, Omar; Treviño, Claudia Lydia; Hernández-Cruz, Arturo; Darszon, Alberto

    2014-01-01

    ABSTRACT The spermatozoa acrosome reaction (AR) is essential for mammalian fertilization. Few methods allow visualization of AR in real time together with Ca2+ imaging. Here, we show that FM4-64, a fluorescent dye used to follow exocytosis, reliably reports AR progression induced by ionomycin and progesterone in human spermatozoa. FM4-64 clearly delimits the spermatozoa contour and reports morphological cell changes before, during, and after AR. This strategy unveiled the formation of moving tubular appendages, emerging from acrosome-reacted spermatozoa, which was confirmed by scanning electron microscopy. Alternate wavelength illumination allowed concomitant imaging of FM4-64 and Fluo-4, a Ca2+ indicator. These AR and intracellular Ca2+ ([Ca2+]i) recordings revealed that the presence of [Ca2+]i oscillations, both spontaneous and progesterone induced, prevents AR in human spermatozoa. Notably, the progesterone-induced AR is preceded by a second [Ca2+]i peak and ∼40% of reacting spermatozoa also manifest a slow [Ca2+]i rise ∼2 min before AR. Our findings uncover new AR features related to [Ca2+]i. PMID:25100708

  13. Characterization of Ca2+ Signalling in Postnatal Mouse Retinal Ganglion Cells: Involvement of OPA1 in Ca2+ Clearance

    Czech Academy of Sciences Publication Activity Database

    Dayanithi, Govindan; Chen-Kuo-Chang, M.; Viero, C.; Hamel, C.; Muller, A.; Lenaers, G.

    2010-01-01

    Roč. 31, č. 2 (2010), s. 53-65 ISSN 1381-6810 Institutional research plan: CEZ:AV0Z50390703 Keywords : retinal ganglion cells * Ca2+ homeostasis * Ca2+ clearance Subject RIV: FH - Neurology Impact factor: 1.333, year: 2010

  14. Serum CA 125, carcinoembryonic antigen, and CA 19-9 as tumor markers in borderline ovarian tumors

    NARCIS (Netherlands)

    Engelen, MJA; de Bruijn, HWA; Hollema, H; ten Koor, KA; Willemse, PHB; Aalders, JG; van der Zee, AGJ

    Objectives. The goals of this study were to analyze preoperative serum levels of CA 125, carcinoembryonic antigen (CEA), and CA 19-9 in patients with borderline ovarian tumors and to investigate if routine assessment of these markers in follow-up may lead to earlier detection of recurrence. Methods.

  15. Thermoelectric properties of antiperovskite calcium oxides Ca3PbO and Ca3SnO

    Science.gov (United States)

    Okamoto, Y.; Sakamaki, A.; Takenaka, K.

    2016-05-01

    We report the thermoelectric properties of polycrystalline samples of Ca3Pb1-xBixO (x = 0, 0.1, 0.2) and Ca3SnO, both crystallizing in a cubic antiperovskite-type structure. The Ca3SnO sample shows metallic resistivity and its thermoelectric power approaches 100 μV K-1 at room temperature, resulting in the thermoelectric power factor of Ca3SnO being larger than that of Ca3Pb1-xBixO. On the basis of Hall and Sommerfeld coefficients, the Ca3SnO sample is found to be a p-type metal with a carrier density of ˜1019 cm-3, a mobility of ˜80 cm2 V-1 s-1, both comparable to those in degenerated semiconductors, and a moderately large hole carrier effective mass. The coexistence of moderately high mobility and large effective mass observed in Ca3SnO, as well as possible emergence of a multivalley electronic structure with a small band gap at low-symmetry points in k-space, suggests that the antiperovskite Ca oxides have strong potential as a thermoelectric material.

  16. Solidification and Immobilization of Heavy metals in Soil using with nano-metallic Ca/CaO Dispersion Mixture

    Directory of Open Access Journals (Sweden)

    Mallampati S. R.

    2013-04-01

    Full Text Available In the present work, the use of nano-metallic calcium (Ca and calcium oxide (CaO dispersion mixture for the immobilization of heavy metals (As, Cd, Cr and Pb in soil was investigated. With simple grinding, 85-90% of heavy metals immobilization could be achieved, while it could be enhanced to 98-100% by grinding with the addition of nano-metallic Ca/CaO dispersion mixture. By SEM-EDS elemental maps as well as semi-quantitative analysis observed that the amount of As, Cd, Cr and Pb measurable on soil particle surface decreases after nano-metallic Ca/CaO treatment. The leachable heavy metals concentrations were reduced, to the concentration lower than the Japan soil elution standard regulatory threshold, i. e., < 0.01 mg/l for As, Cd and Pb and 0.05mg/l for Cr. Whereas, the effect of soil moisture and pH on heavy metals immobilization was not much influenced. The results suggest that nano-metallic Ca/CaO mixture is suitable to be used for the gentle immobilization of heavy metals contaminated soil at normal moisture conditions.

  17. The effects of CaCl2 and CaBr2 on the reproduction of Daphnia magna Straus.

    Science.gov (United States)

    Mažuran, Neda; Hršak, Vladimir; Kovačević, Goran

    2015-06-01

    Concentrated CaCl2 and CaBr2 salt solutions of densities up to 2.3 kg L-1 are regularly used to control hydrostatic pressure in oil wells during special operations in the exploration and production of natural gas and crude oil. Various concentrations of high density salts are frequently left in mud pits near the drilling site as waste, polluting fresh and ground waters by spillage and drainage. The toxic effects of these salts have already been observed. This study investigated the effects of CaCl2 and CaBr2 on water flea Daphnia magna Straus in a 21-day reproduction test. The three tested concentrations of CaCl2 (240, 481, and 1925 mg L-1) caused a significant dose-response decrease of reproduction (psubstance. The results suggest that high concentrations of the tested chemicals are harmful to Daphnia's reproduction and could reduce its abundance.

  18. Expression of PIK3CA, PTEN mRNA and PIK3CA mutations in primary breast cancer

    DEFF Research Database (Denmark)

    Palimaru, Irina; Brügmann, Anja; Wium-Andersen, Marie Kim

    2013-01-01

    suppressor, phosphatase and tensin homolog (PTEN), in breast carcinoma tissue and normal breast tissue. Furthermore, we examine whether expression of PIK3CA and PTEN mRNA and occurrence of PIK3CA mutations are associated with lymph node metastases in patients with primary breast cancer. METHODS: Paired...... tissue samples of breast carcinoma and normal breast tissue were obtained from 175 breast cancer patients at the time of primary surgery, of these 105 patients were lymph node positive. Expression of PIK3CA and PTEN mRNA was quantified with Quantitative Real Time PCR. Somatic mutations in exon 9 and exon...... 20 of the PIK3CA gene were identified by genotyping. RESULTS: Both PIK3CA and PTEN mRNA expression was significantly increased in breast carcinoma tissue compared to normal breast tissue (p = 2 × 10(-11)) and (p 

  19. Neutron-scattering studies of the doubly magic Ca nuclei, 40Ca and 48Ca, in the low-energy resonance region

    International Nuclear Information System (INIS)

    Hicks, S.F.

    1988-01-01

    Measurements of neutron elastic and inelastic scattering differential cross sections from 48 Ca at 2.35, 2.43, 2.71, 3.15, 3.55, 4.70, 6.00, and 7.97 MeV are presented. These data are combined with n + 48 Ca total cross sections taken at Oak Ridge National Laboratory to form a rather extensive set of scattering data for 48 Ca between 80 keV and 20 MeV incident neutron energy. Coupled-channels analyses of these data with a first-order vibrational model described both the neuron inelastic cross sections at 7.97 MeV and intermediate structure resonances observed in the total cross sections below 3 MeV. Particle-core coupled resonance configurations were determined for four intermediate structure resonances from the coupled-channels calculations. Also measured at each of the energies listed above were neutron elastic and inelastic cross sections from 40 Ca. In addition to the above coupled-channels analysis of 48 Ca, the mean field potential used to describe the bound-state and scattering data of 48 Ca was determined using a dispersion relation to connect the real and imaginary components of the optical model potential. Finally, differential scattering cross sections measured between 2 and 3.5 MeV from 48 Ca are compared to calculations using phase shifts from an evaluation of the total cross sections. The agreement between the measured and calculated differential scattering cross sections was excellent at some energies

  20. Si estoy en tu recuerdo soy parte de tu historia

    OpenAIRE

    Boixader i Corominas, Agnès

    2008-01-01

    Partiendo de la constatación de que la historia es transmisora de actitudes, valores e ideología, siempre sujeta a interpretaciones nuevas, y partiendo, asimismo, del valor que tiene para la historia el recuerdo de algunos testimonios de los hechos, se justifica y expone una experiencia didáctica llevada a cabo con jóvenes de bachillerato.

  1. Mechanical and thermal-expansion characteristics of Ca10(PO46(OH2-Ca3(PO42 composites

    Directory of Open Access Journals (Sweden)

    Ruseska G.

    2006-01-01

    Full Text Available Three types of composites consisting of Ca10(PO46(OH2 and Ca3(PO42 with composition: 75% (wt Ca10(PO46(OH2: 25%(wt Ca3(PO42; 50%(wt Ca10(PO46(OH2: 50%(wtCa3(PO42 and 25 %(wt Ca10(PO46(OH2: 75%(wt Ca3(PO42 were the subject of our investigation. Sintered compacts were in thermal equilibrium, which was proved by the absence of hysteresis effect of the dependence ΔL/L=f(T during heating /cooling in the temperature interval 20-1000-200C. Sintered compacts with the previously mentioned composition possess 26-50% higher values of the E-modulus, G-modulus and K-modulus indicating the presence of a synergism effect. Several proposed model equations for predicting the thermal expansion coefficient in dependence of the thermal and elastic properties of the constitutive phases and their volume fractions, given by: Turner, Kerner, Tummala and Friedberg, Thomas and Taya, were used for making correlations between mechanical and thermal-expansion characteristics of the Ca10(PO46(OH2 - Ca3(PO42 composites. Application of the previously mentioned model equations to all kinds of composites leads to the conclusion that the experimentally obtained results for the thermal expansion coefficient are in an excellent agreement with the theoretical calculated values on account of the volume fraction of each constitutive phase and with all applied model equations, with a coefficient of correlation from 98.16-99.86 %.

  2. Clinical value of preoperative serum CA 19-9 and CA 125 levels in predicting the resectability of hilar cholangiocarcinoma.

    Science.gov (United States)

    Hu, Hai-Jie; Mao, Hui; Tan, Yong-Qiong; Shrestha, Anuj; Ma, Wen-Jie; Yang, Qin; Wang, Jun-Ke; Cheng, Nan-Sheng; Li, Fu-Yu

    2016-01-01

    To examine the predictive value of tumor markers for evaluating tumor resectability in patients with hilar cholangiocarcinoma and to explore the prognostic effect of various preoperative factors on resectability in patients with potentially resectable tumors. Patients with potentially resectable tumors judged by radiologic examination were included. The receiver operating characteristic (ROC) analysis was conducted to evaluate serum carbohydrate antigenic determinant 19-9 (CA 19-9), carbohydrate antigen 125 (CA 125) and carcino embryonie antigen levels on tumor resectability. Univariate and multivariate logistic regression models were also conducted to analysis the correlation of preoperative factors with resectability. In patients with normal bilirubin levels, ROC curve analysis calculated the ideal CA 19-9 cut-off value of 203.96 U/ml in prediction of resectability, with a sensitivity of 83.7 %, specificity of 80 %, positive predictive value of 91.1 % and negative predictive value of 66.7 %. Meanwhile, the optimal cut-off value for CA 125 to predict resectability was 25.905 U/ml (sensitivity, 78.6 %; specificity, 67.5 %). In a multivariate logistic regression model, tumor size ≤3 cm (OR 4.149, 95 % CI 1.326-12.981, P = 0.015), preoperative CA 19-9 level ≤200 U/ml (OR 20.324, 95 % CI 6.509-63.467, P CA 125 levels ≤26 U/ml (OR 8.209, 95 % CI 2.624-25.677, P CA 19-9 and CA 125 levels predict resectability in patients with radiological resectable hilar cholangiocarcinoma. Increased preoperative CA 19-9 levels and CA 125 levels are associated with poor resectability rate.

  3. Ca intercalated bilayer graphene as a thinnest limit of superconducting C6Ca

    Science.gov (United States)

    Kanetani, Kohei; Sugawara, Katsuaki; Sato, Takafumi; Shimizu, Ryota; Iwaya, Katsuya; Hitosugi, Taro; Takahashi, Takashi

    2012-01-01

    Success in isolating a 2D graphene sheet from bulky graphite has triggered intensive studies of its physical properties as well as its application in devices. Graphite intercalation compounds (GICs) have provided a platform of exotic quantum phenomena such as superconductivity, but it is unclear whether such intercalation is feasible in the thinnest 2D limit (i.e., bilayer graphene). Here we report a unique experimental realization of 2D GIC, by fabricating calcium-intercalated bilayer graphene C6CaC6 on silicon carbide. We have investigated the structure and electronic states by scanning tunneling microscopy and angle-resolved photoemission spectroscopy. We observed a free-electron–like interlayer band at the Brillouin-zone center, which is thought to be responsible for the superconductivity in 3D GICs, in addition to a large π* Fermi surface at the zone boundary. The present success in fabricating Ca-intercalated bilayer graphene would open a promising route to search for other 2D superconductors as well as to explore its application in devices. PMID:23139407

  4. Changes to a CA Programme - Practitioners' Perspectives

    Directory of Open Access Journals (Sweden)

    Elizabeth Wheeler

    2014-03-01

    Full Text Available This paper examines the perceptions of practitioners of the new directions taken by NZICA with respect to its academic and professional programme requirements to obtain CA Institute membership. The “future viability of any professional body is dependent on continuously attracting new members, ideally the best and the brightest new tertiary graduates”, and this is “undoubtedly the case for New Zealand’s professional accounting body, the New Zealand Institute of Chartered Accountants (NZICA” (Malthus & Fowler 2009, p. 26. In this study, the concurrent triangulation approach to mixed methods described by Creswell (2009 was used to collect data. This approach enabled the results of the two quantitative and qualitative databases to be integrated and compared. It was found that accounting practitioners felt the changes made by NZICA may devalue the brand, while the reduction in liberal papers would result in a narrower degree. Overall, accounting practitioners agreed that three to four years of tertiary accounting education was adequate, a broader four-year course would result in a better-rounded graduates. The reduction in the length of the tertiary programme caused concern that future graduates would be less mature. Accounting practitioners also felt that the changes would harm the credibility of NZICA internationally. However, some accounting practitioners did welcome the fact that the NZICA membership requirements will be more aligned with Institute of Chartered Accountants Australia. Accounting practitioners felt that the new technical modules would offer more flexibility. They believed that the four technical modules should repeat the material taught at university, as long as a balance was maintained between technical and practical skills. They also believed that the changes would result in an increased onus on the employer. Additionally, accounting practitioners agreed that on-the-job training should not replace a tertiary

  5. Shock Compression Response of Calcium Fluoride (CaF2)

    Science.gov (United States)

    Root, Seth

    2017-06-01

    The fluorite crystal structure is a textbook lattice that is observed for many systems, such as CaF2, Mg2 Si, and CeO2. Specifically, CaF2 is a useful material for studying the fluorite system because it is readily available as a single crystal. Under static compression, CaF2 is known to have at least three solid phases: fluorite, cotunnite, and a Ni2 In phase. Along the Hugoniot CaF2 undergoes a fluorite to cotunnite phase transition, however, at higher shock pressures it is unknown whether CaF2 undergoes another solid phase transition or melts directly from the cotunnite phase. In this work, we conducted planar shock compression experiments on CaF2 using Sandia's Z-machine and a two-stage light gun up to 900 GPa. In addition, we use density functional theory (DFT) based quantum molecular dynamics (QMD) simulations to provide insight into the CaF2 state along the Hugoniot. In collaboration with: Michael Desjarlais, Ray Lemke, Patricia Kalita, Scott Alexander, Sandia National Laboratories. Sandia National Laboratories is a multi-mission laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL850.

  6. Study of 51Ca via three-neutron transfer

    International Nuclear Information System (INIS)

    Catford, W.N.; Fifield, L.K.; Ophel, T.R.; Orr, N.A.; Weisser, D.C.; Woods, C.L.

    1988-09-01

    Low-lying levels in 51 Ca have been studied via the reaction 48 Ca( 18 O, 15 O) 51 Ca at a beam energy of 108 MeV. Recoiling 51 Ca nuclei were detected in coincidence with 15 O ejectiles, largely removing the substantial background from target contaminants that was encountered in previous work. The lowest 51C a level identified is 1.01 ± 0.11 MeV less bound than the ground state, according to a recent measurement of the ground state mass using the 48 Ca( 14 C, 11 C) 51 Ca reaction. Considerations of the difficulty in identifying the ground state peak suggest that published values of the mass excess should be viewed with caution, and that a definitive mass measurement is still required in order to resolve questions on the structure of the neutron rich calcium isotopes. A total of six energy levels in 51 Ca have been identified and their mass excesses measured. Only two of these levels have been observed previously, via the ( 14 C, 11 C) reaction. Striking differences in selectivity between the ( 18 O, 15 O) and ( 14 C, 11 C) transfer reactions are discussed

  7. Ca2+ signalling early in evolution--all but primitive.

    Science.gov (United States)

    Plattner, Helmut; Verkhratsky, Alexei

    2013-05-15

    Early in evolution, Ca(2+) emerged as the most important second messenger for regulating widely different cellular functions. In eukaryotic cells Ca(2+) signals originate from several sources, i.e. influx from the outside medium, release from internal stores or from both. In mammalian cells, Ca(2+)-release channels represented by inositol 1,4,5-trisphosphate receptors and ryanodine receptors (InsP3R and RyR, respectively) are the most important. In unicellular organisms and plants, these channels are characterised with much less precision. In the ciliated protozoan, Paramecium tetraurelia, 34 molecularly distinct Ca(2+)-release channels that can be grouped in six subfamilies, based on criteria such as domain structure, pore, selectivity filter and activation mechanism have been identified. Some of these channels are genuine InsP3Rs and some are related to RyRs. Others show some--but not all--features that are characteristic for one or the other type of release channel. Localisation and gene silencing experiments revealed widely different--yet distinct--localisation, activation and functional engagement of the different Ca(2+)-release channels. Here, we shall discuss early evolutionary routes of Ca(2+)-release machinery in protozoa and demonstrate that detailed domain analyses and scrutinised functional analyses are instrumental for in-depth evolutionary mapping of Ca(2+)-release channels in unicellular organisms.

  8. Aerobic Hydrogen Production via Nitrogenase in Azotobacter vinelandii CA6

    Science.gov (United States)

    Noar, Jesse; Loveless, Telisa; Navarro-Herrero, José Luis; Olson, Jonathan W.

    2015-01-01

    The diazotroph Azotobacter vinelandii possesses three distinct nitrogenase isoenzymes, all of which produce molecular hydrogen as a by-product. In batch cultures, A. vinelandii strain CA6, a mutant of strain CA, displays multiple phenotypes distinct from its parent: tolerance to tungstate, impaired growth and molybdate transport, and increased hydrogen evolution. Determining and comparing the genomic sequences of strains CA and CA6 revealed a large deletion in CA6's genome, encompassing genes related to molybdate and iron transport and hydrogen reoxidation. A series of iron uptake analyses and chemostat culture experiments confirmed iron transport impairment and showed that the addition of fixed nitrogen (ammonia) resulted in cessation of hydrogen production. Additional chemostat experiments compared the hydrogen-producing parameters of different strains: in iron-sufficient, tungstate-free conditions, strain CA6's yields were identical to those of a strain lacking only a single hydrogenase gene. However, in the presence of tungstate, CA6 produced several times more hydrogen. A. vinelandii may hold promise for developing a novel strategy for production of hydrogen as an energy compound. PMID:25911479

  9. Abnormal CA-125 levels in menopausal women without ovarian cancer.

    Science.gov (United States)

    Terada, Keith Y; Elia, Jennifer; Kim, Robert; Carney, Michael; Ahn, Hyeong Jun

    2014-10-01

    To determine if an abnormal CA-125 level in a menopausal female without ovarian cancer is associated with an increase in mortality. The Prostate, Lung, Colorectal, and Ovarian Cancer Screening Randomized Controlled (PLCO) Trial is a large multicenter prospective trial conducted by the National Cancer Institute (NCI). Over 78,000 healthy women aged 55-74 were randomized to a screening arm versus a usual medical care arm to evaluate the efficacy of screening in reducing mortality due to ovarian cancer. Women in the screening arm underwent annual screening for ovarian cancer with transvaginal ultrasound and CA-125 levels. There were 38,818 patients without ovarian cancer that had at least one CA-125 level drawn; 1201 (3.09%) had at least one abnormal level. The current study compares mortality in patients that had one or more abnormal CA-125 levels without ovarian cancer versus those with all normal levels. Patients with one or more abnormal CA-125 levels, without ovarian cancer, had a significantly higher mortality than patients with all normal CA-125 levels in the PLCO screening trial (pCA-125 and without ovarian cancer are exposed to an increased risk of premature mortality. Copyright © 2014 Elsevier Inc. All rights reserved.

  10. Menstrual Cycle Dependent Variability for Serum Tumor Markers CEA, AFP, CA 19-9, CA 125 and CA 15-3 in Healthy Women

    Directory of Open Access Journals (Sweden)

    Ayşe Binnur Erbağci

    1999-01-01

    Full Text Available Information on menstrual cycle dependent variation of tumor markers in healthy women is a subject of diagnostic efficiency and has an impact in elucidating the normal function of these markers. In this study midfollicular and midluteal concentrations of serum CEA, AFP, CA 19-9, CA 125, CA 15-3 and their relations with LH, FSH, prolactin, estradiol and progesterone were evaluated during ovulatory cycles in a group of 23 healthy female individuals. Samples were collected on the 7th and 21st day of the same menstrual cycle. Tumor marker and hormone concentrations were determined with chemiluminescence or electrochemiluminescence EIA methods. A significant phase-dependent difference was observed for CA 15-3, midluteal concentrations (mean ± SEM; 26.33 ± 1.56 U/ml higher than the midfollicular (mean ± SEM; 19.27 ± 1.49 U/ml concentrations (p < 0.001. But an obvious difference for other tumor markers investigated did not exist. Significant correlations of follicular and luteal CA 125 levels with body mass index of the subjects were observed (r:0.52, p < 0.05 and r:0.57, p < 0.005, respectively.

  11. Human chorionic gonadotropin and CA 15-3 producing adenocarcinoma

    International Nuclear Information System (INIS)

    Ueckaya, G.; Oezet, A.; Arpaci, A.; Koemuercue, S.

    1998-01-01

    50 years old man suffering from primary lung adenocarcinoma presented with high levels of both beta subunit human chorionic gonadotropin (βHCG) and cancer antigen 15-3 (CA 15-3) in the absence of elevated carcinoembrionic antigen (CEA), alfa fetoprotein (AFP) and carbohydrate antigen 19-9 (CA 19-9). Although βHCG or CA 15-3 high levels were reported in adenocarcinoma of lung, this is the first report of a patient with high levels of both markers. (orig.) [de

  12. Ca4As3 – a new binary calcium arsenide

    Science.gov (United States)

    Hoffmann, Andrea V.; Hlukhyy, Viktor; Fässler, Thomas F.

    2015-01-01

    The crystal structure of the binary compound tetra­calcium triarsenide, Ca4As3, was investigated by single-crystal X-ray diffraction. Ca4As3 crystallizes in the Ba4P3 structure type and is thus a homologue of isotypic Sr4As3. The unit cell contains 32 Ca2+ cations, 16 As3− isolated anions and four centrosymmetric [As2]4– dumbbells. The As atoms in each of the dumbbells are connected by a single bond, thus this calcium arsenide is a Zintl phase. PMID:26870427

  13. Ca4As3 – a new binary calcium arsenide

    Directory of Open Access Journals (Sweden)

    Andrea V. Hoffmann

    2015-12-01

    Full Text Available The crystal structure of the binary compound tetracalcium triarsenide, Ca4As3, was investigated by single-crystal X-ray diffraction. Ca4As3 crystallizes in the Ba4P3 structure type and is thus a homologue of isotypic Sr4As3. The unit cell contains 32 Ca2+ cations, 16 As3− isolated anions and four centrosymmetric [As2]4– dumbbells. The As atoms in each of the dumbbells are connected by a single bond, thus this calcium arsenide is a Zintl phase.

  14. Luz, yo soy tu padre

    OpenAIRE

    Sánchez Martín, Rubén

    2013-01-01

    Se presenta un pack de autopromoción. Son un recurso común entre la comunidad creativa como forma de demostrar sus habilidades en diferentes formatos y buscar un contacto original y agradable con el receptor a la hora de considerar a un candidato para un puesto laboral o la concesión de un proyecto. Se abordan desde el diseño de packaging a la dirección de arte de vídeo, pasando por el diseño de publicaciones, la redacción creativa, la conceptualización, la creación de identidad visual, el di...

  15. TU Delft en online media

    NARCIS (Netherlands)

    Speekenbrink, R.J.H.

    2013-01-01

    Dit boek behandelt een visie op online media bij Nederlandse universiteiten. Daarbij beperk ik me tot die aspecten waarin online communicatie en/of online marketing een rol speelt. Dit boek behandelt niet de onderwijsgerelateerde aspecten van online media. Elektronische leeromgevingen, sociale media

  16. βCaMKII plays a nonenzymatic role in hippocampal synaptic plasticity and learning by targeting αCaMKII to synapses.

    Science.gov (United States)

    Borgesius, Nils Z; van Woerden, Geeske M; Buitendijk, Gabrielle H S; Keijzer, Nanda; Jaarsma, Dick; Hoogenraad, Casper C; Elgersma, Ype

    2011-07-13

    The calcium/calmodulin-dependent kinase type II (CaMKII) holoenzyme of the forebrain predominantly consists of heteromeric complexes of the αCaMKII and βCaMKII isoforms. Yet, in contrast to αCaMKII, the role of βCaMKII in hippocampal synaptic plasticity and learning has not been investigated. Here, we compare two targeted Camk2b mouse mutants to study the role of βCaMKII in hippocampal function. Using a Camk2b(-/-) mutant, in which βCaMKII is absent, we show that both hippocampal-dependent learning and Schaffer collateral-CA1 long-term potentiation (LTP) are highly dependent upon the presence of βCaMKII. We further show that βCaMKII is required for proper targeting of αCaMKII to the synapse, indicating that βCaMKII regulates the distribution of αCaMKII between the synaptic pool and the adjacent dendritic shaft. In contrast, localization of αCaMKII, hippocampal synaptic plasticity and learning were unaffected in the Camk2b(A303R) mutant, in which the calcium/calmodulin-dependent activation of βCaMKII is prevented, while the F-actin binding and bundling property is preserved. This indicates that the calcium/calmodulin-dependent kinase activity of βCaMKII is fully dispensable for hippocampal learning, LTP, and targeting of αCaMKII, but implies a critical role for the F-actin binding and bundling properties of βCaMKII in synaptic function. Together, our data provide compelling support for a model of CaMKII function in which αCaMKII and βCaMKII act in concert, but with distinct functions, to regulate hippocampal synaptic plasticity and learning.

  17. Controls over δ44/40Ca and Sr/Ca variations in coccoliths: New perspectives from laboratory cultures and cellular models

    Science.gov (United States)

    Mejía, Luz María; Paytan, Adina; Eisenhauer, Anton; Böhm, Florian; Kolevica, Ana; Bolton, Clara; Méndez-Vicente, Ana; Abrevaya, Lorena; Isensee, Kirsten; Stoll, Heather

    2018-01-01

    Coccoliths comprise a major fraction of the global carbonate sink. Therefore, changes in coccolithophores' Ca isotopic fractionation could affect seawater Ca isotopic composition, affecting interpretations of the global Ca cycle and related changes in seawater chemistry and climate. Despite this, a quantitative interpretation of coccolith Ca isotopic fractionation and a clear understanding of the mechanisms driving it are not yet available. Here, we address this gap in knowledge by developing a simple model (CaSri-Co) to track coccolith Ca isotopic fractionation during cellular Ca uptake and allocation to calcification. We then apply it to published and new δ 44 / 40 Ca and Sr/Ca data of cultured coccolithophores of the species Emiliania huxleyi and Gephyrocapsa oceanica. We identify changes in calcification rates, Ca retention efficiency and solvation-desolvation rates as major drivers of the Ca isotopic fractionation and Sr/Ca variations observed in cultures. Higher calcification rates, higher Ca retention efficiencies and lower solvation-desolvation rates increase both coccolith Ca isotopic fractionation and Sr/Ca. Coccolith Ca isotopic fractionation is most sensitive to changes in solvation-desolvation rates. Changes in Ca retention efficiency may be a major driver of coccolith Sr/Ca variations in cultures. We suggest that substantial changes in the water structure strength caused by past changes in temperature could have induced significant changes in coccolithophores' Ca isotopic fractionation, potentially having some influence on seawater Ca isotopic composition. We also suggest a potential effect on Ca isotopic fractionation via modification of the solvation environment through cellular exudates, a hypothesis that remains to be tested.

  18. Delta(9)-tetrahydrocannabinol activates [Ca2+], increases partly sensitive to capacitative store refilling

    NARCIS (Netherlands)

    Filipeanu, CM; deZeeuw, D; Nelemans, SA

    1997-01-01

    Delta(9)-Tetrahydrocannabinol induces [Ca2+](i) increases in DDT1MF-2 smooth muscle cells. Both Ca2+ entry and release from intracellular Ca2+ stores were concentration dependently activated. The Ca2+ entry component contributed most to the increases in [Ca2+](i). Stimulation with

  19. Acid mist and soil Ca and Al alter the mineral nutrition and physiology of red spruce

    Science.gov (United States)

    P.G. Schaberg; D.H. DeHayes; G.J. Hawley; G.R. Strimbeck; J.R. Cumming; P.F. Murakami; C.H. Borer

    2000-01-01

    We examined the effects and potential interactions of acid mist and soil solution Ca and Al treatments on foliar cation concentrations, membrane-associated Ca (mCa), ion leaching, growth, carbon exchange, and cold tolerance of red spruce (Picea rubens Sarg.) saplings. Soil solution Ca additions increased foliar Ca and Zn concentrations, and increased...

  20. Control efficacy of Ca-containing foliar fertilizers on bitter pit in bagged 'Fuji' apple and effects on the Ca and N contents of apple fruits and leaves.

    Science.gov (United States)

    Yu, Xian-Mei; Wang, Jin-Zheng; Nie, Pei-Xian; Xue, Xaio-Min; Wang, Gui-Ping; An, Miao

    2018-04-20

    The preharvest application of Ca-containing foliar fertilizers can reduce bitter pit (BP) incidence in apples and improve fruit quality by increasing the Ca content and decreasing both the N content and the N/Ca ratio in fruits. In this study, we aimed to investigate the control efficacy of Ca-containing fertilizers on BP incidence and the effects on the Ca and N contents in bagged 'Fuji' apple by spraying the foliar fertilizer containing calcium chloride (CaCl 2 ), calcium nitrate [Ca(NO 3 ) 2 ] or calcium formate [Ca(HCOO) 2 ] at early stage [5 days after full bloom (DAFB) + 40 DAFB] and at late stage (80 DAFB + 125 DAFB). The BP incidences were reduced significantly with the reduce percentage of 43.2~73.0%, and the efficacy of spraying at early stage was significantly higher than that of spraying at late stage. The Ca content of bagged apple fruits was increased while the N content and N/Ca ratio were decreased after spraying Ca-containing foliar fertilizers, however, the Ca content, N content and N/Ca ratio of apple leaves were differentially influenced. The foliar fertilizer containing CaCl 2, Ca(NO 3 ) 2 or Ca(HCOO) 2 could be used at early stage to control BP in apple and improve the quality of bagged apple fruits. This article is protected by copyright. All rights reserved.

  1. Substitution site and effects on magnetism in Sr-for-Ca substituted CaBaCo4O7

    Science.gov (United States)

    Aurelio, G.; Curiale, J.; Bardelli, F.; Junqueira Prado, R.; Hennet, L.; Cuello, G.; Campo, J.; Thiaudière, D.

    2015-10-01

    Cationic substitutions in the novel magnetoelectric compound CaBaCo4O7 lead to profound changes in its magnetic and electric behaviors. In this work, we present a structural study of the isovalent substitution Sr-for-Ca in CaBaCo4O7. X-ray diffraction, as well as neutron powder diffraction experiments, are reported for a series of samples Ca1-xSrxBaCo4O7 with 0 ≤ x ≤ 0.10 . Special emphasis is given to the identification of the substitution site, as Sr has also been reported to substitute for Ba in this crystal structure. The solubility limit for Sr at the Ca site is shown to be at x ≃ 0.08 . The variation of lattice constants with Sr-doping firmly supports the Sr-for-Ca substitution. Rietveld refinements of the Sr-substituted samples are presented, and used as starting point to analyse the local structure around Sr by means of X-ray absorption spectroscopy at the Sr K-edge. Both the near-edge absorption and the extended absorption fine-structure confirm the substitution of Sr for Ca, giving definite support to the proposed nominal formula. In addition, macroscopic magnetization measurements are presented which reveal the striking effects of Sr-substitution over the magnetic landscape of this puzzling compound.

  2. Towards reconstructing ancient seawater Mg/Ca by combining porcelaneous and hyaline foraminiferal Mg/Ca-temperature calibrations

    Science.gov (United States)

    Wit, J. C.; de Nooijer, L. J.; Haig, J.; Jorissen, F. J.; Thomas, E.; Reichart, G.-J.

    2017-08-01

    The temperature of the deep ocean plays a vital role in the Earth's climate system. Paleo-reconstructions of deep-sea temperatures have traditionally been based on the oxygen isotope composition of deep-sea benthic foraminiferal calcite shells, although this parameter depends upon polar ice volume as well as temperature. More recent reconstructions use Mg/Ca in these shells, with temperature calibrations based on empirical relationships observed in present-day oceans. Incorporation of Mg (DMg) into foraminiferal calcite is, however, not solely dependent on temperature, but also on seawater Mg/Ca. Due to its long oceanic residence time, Mg concentrations remained relatively constant over time scales of a few hundred thousand years, but varied significantly over longer geological time scales. Accurate reconstruction of past temperatures using foraminiferal Mg/Ca, therefore, hinges on our understanding of Mg/Ca seawater changes on geological timescales. We explore a novel, independent approach to reconstructing past seawater Mg/Ca using the temperature-dependent offset in DMg between porcelaneous (secreting intermediate- or high-Mg calcite, abbreviated as IMC or HMC, respectively) and hyaline (producing low-Mg calcite, abbreviated as LMC) benthic foraminifera. We calibrated the Mg/Ca-temperature dependence for Pyrgo spp. (one of the few common, large-sized porcelaneous taxa present in the deep-sea since the middle Miocene), and combined this with an existing calibration of hyaline Cibicidoides spp. to mathematically solve for changes in Mg/Ca seawater through time. We show that changes in Mg/Ca seawater can be reconstructed using the offset between porcelaneous and hyaline foraminifera, but absolute values are highly dependent on the species-specific offset between Mg/Ca seawater and Mg-partition coefficients.

  3. Temperature and Ca2+-dependence of the sarcoplasmic reticulum Ca2(+)-ATPase in haddock, salmon, rainbow trout and zebra cichlid

    DEFF Research Database (Denmark)

    Godiksen, Helene; Jessen, Flemming

    2002-01-01

    in the enzyme or its membrane lipid environment is still a matter of discussion. In this study we compared the temperature dependence and Ca2+-dependence of SR Ca2+-ATPase in haddock (Melanogrammus aeglefinus), salmon (Salmo, salar), rainbow trout (Oncorhynchus mykiss) and zebra cichlid (Cichlasoma...... nigrofasciatum). The Arrhenius plot of zebra cichlid showed a break point at 20 degreesC, and the haddock Arrhenius plot was non-linear with pronounced changes in slope in the. temperature area, 6-14 degreesC. In Arrhenius plot from both salmon and rainbow trout a plateau exists with an almost constant SR Ca2...

  4. Anandamide reduces intracellular Ca2+ concentration through suppression of Na+/Ca2+ exchanger current in rat cardiac myocytes.

    Directory of Open Access Journals (Sweden)

    Qian Li

    Full Text Available Anandamide, one of the endocannabinoids, has been reported to exhibit cardioprotective properties, particularly in its ability to limit the damage produced by ischemia reperfusion injury. However, the mechanisms underlying the effect are not well known. This study is to investigate whether anandamide alter Na(+/Ca(2+ exchanger and the intracellular free Ca(2+ concentration ([Ca(2+]i.Na(+/Ca(2+ exchanger current (I(NCX was recorded and analysed by using whole-cell patch-clamp technique and [Ca(2+]i was measured by loading myocytes with the fluorescent Ca(2+ indicator Fura-2/AM.We found that I(NCX was enhanced significantly after perfusion with simulated ischemic external solution; [Ca(2+]i was also significantly increased by simulated ischemic solution. The reversal potential of I(NCX was shifted to negative potentials in simulated ischemic external solution. Anandamide (1-100 nM failed to affect I(NCX and [Ca(2+]i in normal solution. However, anandamide (1-100 nM suppressed the increase in INCX in simulated ischemic external solution concentration-dependently and normalized INCX reversal potential. Furthermore, anandamide (100 nM significantly attenuated the increase in [Ca(2+]i in simulated ischemic solution. Blocking CB1 receptors with the specific antagonist AM251 (500 nM failed to affect the effects of anandamide on I(NCX and [Ca(2+]i in simulated ischemic solution. CB2 receptor antagonist AM630 (100 nM eliminated the effects of anandamide on I(NCX and [Ca(2+]i in simulated ischemic solution, and CB2 receptor agonist JWH133 (100 nM simulated the effects of anandamide that suppressed the increase in I(NCX and [Ca(2+]i in simulated ischemic solution. In addition, pretreatment with the Gi/o-specific inhibitor pertussis toxin (PTX, 500 ng/ml eliminated the effects of anandamide and JWH133 on I(NCX in simulated ischemic solution.Collectively, these findings suggest that anandamide suppresses calcium overload through inhibition of I(NCX during

  5. Structural and compositional characterization of synthetic (Ca,Sr)-tremolite and (Ca,Sr)-diopside solid solutions

    Science.gov (United States)

    Gottschalk, M.; Najorka, J.; Andrut, M.

    Tremolite (CaxSr1-x)2Mg5[Si8O22/(OH)2] and diopside (CaxSr1-x)Mg[Si2O6] solid solutions have been synthesized hydrothermally in equilibrium with a 1 molar (Ca,Sr)Cl2 aqueous solution at 750°C and 200 MPa. The solid run products have been investigated by optical, electron scanning and high resolution transmission electron microscopy, electron microprobe, X-ray-powder diffraction and Fourier-transform infrared spectroscopy. The synthesized (Ca,Sr)-tremolites are up to 2000 µm long and 30 µm wide, the (Ca,Sr)-diopsides are up to 150 µm long and 20 µm wide. In most runs the tremolites and diopsides are well ordered and chain multiplicity faults are rare. Nearly pure Sr-tremolite (tr0.02Sr-tr0.98) and Sr-diopside (di0.01Sr-di0.99) have been synthesized. A continuous solid solution series, i.e. complete substitution of Sr2+ for Ca2+ on M4-sites exists for (Ca,Sr)-tremolite. Total substitution of Sr2+ for Ca2+ on M2-sites can be assumed for (Ca,Sr)-diopsides. For (Ca,Sr)-tremolites the lattice parameters a, b and β are linear functions of composition and increase with Sr-content whereas c is constant. For the diopside series all 4 lattice parameters are a linear function of composition; a, b, c increase and β decreases with rising Sr-content. The unit cell volume for tremolite increases 3.47% from 906.68 Å3 for tremolite to 938.21 Å3 for Sr-tremolite. For diopside the unit cell volume increases 4.87 % from 439.91 Å3 for diopside to 461.30 Å3 for Sr-diopside. The observed splitting of the OH stretching band in tremolite is caused by different configurations of the next nearest neighbors (multi mode behavior). Resolved single bands can be attributed to the following configurations on the M4-sites: SrSr, SrCa, CaCa and CaMg. The peak positions of these 4 absorption bands are a linear function of composition. They are shifted to lower wavenumbers with increasing Sr-content. No absorption band due to the SrMg configuration on the M4-site is observed. This indicates

  6. Thermal unfolding of a Ca- and Lanthanide-binding protein

    Energy Technology Data Exchange (ETDEWEB)

    Fahmy, Karim [Helmholtz-Zentrum Dresden-Rossendorf e.V., Dresden (Germany). Biophysics; Goettfert, M. [Technische Univ. Dresden (Germany); Knoeppel, J.

    2017-06-01

    The MIIA (metal ion-induced autocleavage)-domain of the protein Vic001052 from the pathogen Vibrio coralliilyticus, comprises 173 amino acids and exhibits Ca-dependent autoproteolytic activity. It shows homology to nodulation proteins which are secreted by Rhizobiacea into plant host cells where they exert Ca-dependent functions. We have studied the structural and energetic aspects of metal protein interactions of the MIIA domain which appear attractive for engineering metal-binding synthetic peptides. Using a non-cleavable MIIA domain construct, we detected very similar structural changes upon binding to Ca{sup 2+} and Eu{sup 3+}. The thermal denaturation of the Ca-bound state was studied by circular dichroism spectroscopy. The metal-bound folded state unfolds reversibly into an unstructured metal-free state similar to the metal-free state at room temperature.

  7. Neuronal migration and its disorders affecting the CA3 region

    Directory of Open Access Journals (Sweden)

    Richard eBelvindrah

    2014-03-01

    Full Text Available In this review, we focus on CA3 neuronal migration disorders in the rodent. We begin by introducing the main steps of hippocampal development, and we summarize characteristic hippocampal malformations in human. We then describe various mouse mutants showing structural hippocampal defects. Notably, genes identified in human cortical neuronal migration disorders consistently give rise to a CA3 phenotype when mutated in the mouse. We successively describe their molecular, physiological and behavioral phenotypes that together contribute to a better understanding of CA3-dependent functions. We finally discuss potential factors underlying the CA3 vulnerability revealed by these mouse mutants and that may also contribute to other human neurological and psychiatric disorders.

  8. A vaccination strategy to SEIR-CA model

    Science.gov (United States)

    Almuzakki, Muhammad Zaki; Nuraini, Nuning

    2016-04-01

    A combination between Susceptible-Exposed-Infected-Removed (SEIR) model and Cellular Automaton (CA) called SEIR-CA model has been proposed to simulate spreading diseases through populations. We make an improvement to the parameter which describe the impact of neighborhood in CA system. We also propose a vaccination strategy to the model. Three examples are given to illustrate the model. The first one shows that the previously established SEIR-CA model does not work properly in a population with randomly distributed individuals. After an improvement to the neighborhood impact parameter has been made, the model works properly in a population with randomly distributed individuals and it is shown in the second example. The last example shows the spreading mechanisms with a chosen vaccination strategy. We also show that the vaccination strategy can reduce the number of infected individuals and can suppress the spread of the diseases.

  9. Global Database of Borehole Temperatures and Climate Reconstructions - CA-0003

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data collected from borehole site CA-0003. For an accurate assessment of the relative roles of natural variability and anthropogenic influence in the Earth's...

  10. Cell surface topology creates high Ca2+ signalling microdomains

    DEFF Research Database (Denmark)

    Brasen, Jens Christian; Olsen, Lars Folke; Hallett, Maurice B

    2010-01-01

    20 microM Ca2+ to be activated. In this paper, we have resolved this apparent paradox by showing that the surface topology of cells represents an important and hitherto unrecognized feature for generating microdomains of high Ca2+ in cells. We show that whereas the standard modeling assumption...... of a smooth cell surface predicts only moderate localized effects, the more realistic "wrinkled" surface topology predicts that Ca2+ concentrations up to 80 microM can persist within the folds of membranes for significant times. This intra-wrinkle location may account for 5% of the total cell volume. Using......-wrinkle location is also a strategic location at which Ca2+ acts as a regulator of the cortical cytoskeleton and plasma membrane expansion....

  11. Mixing Corrosion of CaCO3 in Natural Waters

    Directory of Open Access Journals (Sweden)

    Qian Hui

    2011-01-01

    Full Text Available The methods of aqueous speciation calculation, pH calculation and the solubility (precipitability calculation of CaCO3 were used to study the mixing corrosion of CaCO3 in natural waters. Mixing processes were done between two unsaturated (with CaCO3 solutions, two oversaturated solutions and between an unsaturated solution and an oversaturated solution, respectively. Results show that the mixing corrosion can be divided into two different levels: mixing corrosion in strict sense and mixing corrosion in broad sense. When mixing corrosion occurs, the HCO3- concentration in one end member solution is usually higher than that in the other solution, and the Ca2+ concentration in the former solution is also usually higher than that in the latter one.

  12. Targeting Cardiomyocyte Ca2+ Homeostasis in Heart Failure

    Science.gov (United States)

    Røe, Åsmund T.; Frisk, Michael; Louch, William E.

    2015-01-01

    Improved treatments for heart failure patients will require the development of novel therapeutic strategies that target basal disease mechanisms. Disrupted cardiomyocyte Ca2+ homeostasis is recognized as a major contributor to the heart failure phenotype, as it plays a key role in systolic and diastolic dysfunction, arrhythmogenesis, and hypertrophy and apoptosis signaling. In this review, we outline existing knowledge of the involvement of Ca2+ homeostasis in these deficits, and identify four promising targets for therapeutic intervention: the sarcoplasmic reticulum Ca2+ ATPase, the Na+-Ca2+ exchanger, the ryanodine receptor, and t-tubule structure. We discuss experimental data indicating the applicability of these targets that has led to recent and ongoing clinical trials, and suggest future therapeutic approaches. PMID:25483944

  13. BCL-2, Ca, and Apoptosis in Breast Cancer

    National Research Council Canada - National Science Library

    Machen, Terry

    1998-01-01

    The central hypothesis of this project is that bcl-2 controls PCD and oncogenesis by altering Ca permeability by the ER/nuclear membranes, mitochondria and/or plasma membrane of mammary epithelial cells...

  14. Deciphering the molecular nature of ovarian cancer biomarker CA125.

    Science.gov (United States)

    Weiland, Florian; Martin, Karina; Oehler, Martin K; Hoffmann, Peter

    2012-01-01

    The ovarian cancer biomarker CA125 has been extensively investigated over the last 30 years. The knowledge about the exact molecular nature of this protein, however, remains fragmented. This review provides an overview of the structural research regarding CA125, and presents an orthogonal verification method to confirm the identity of this molecule. The need for independent identification of CA125 is exemplified by several reports where mutually exclusive data concerning the existence of isoforms and the glycan moieties is presented. Mass spectrometry can overcome the pitfalls of a single detection/identification method such as antibody probing. Independent verification of CA125 identity in characterization studies will help establish a refined model of its molecular structure that will promote the development of new approaches for diagnosis, prognosis and therapy of ovarian cancer.

  15. Deciphering the Molecular Nature of Ovarian Cancer Biomarker CA125

    Directory of Open Access Journals (Sweden)

    Peter Hoffmann

    2012-08-01

    Full Text Available The ovarian cancer biomarker CA125 has been extensively investigated over the last 30 years. The knowledge about the exact molecular nature of this protein, however, remains fragmented. This review provides an overview of the structural research regarding CA125, and presents an orthogonal verification method to confirm the identity of this molecule. The need for independent identification of CA125 is exemplified by several reports where mutually exclusive data concerning the existence of isoforms and the glycan moieties is presented. Mass spectrometry can overcome the pitfalls of a single detection/identification method such as antibody probing. Independent verification of CA125 identity in characterization studies will help establish a refined model of its molecular structure that will promote the development of new approaches for diagnosis, prognosis and therapy of ovarian cancer.

  16. Serum level of tumor marker CA-125 in ovarian pathology

    International Nuclear Information System (INIS)

    Bagni, B.; Feggi, L.M.; Prandini, N.; Pasini, S.; Mollica, G.

    1987-01-01

    The tumor marker CA-125 is an embrional glycoprotein detectable in tissues derived from celomatic epitelium. Serum Ca-125 was determined by RIA in 66 patients with various ovarian pathologies (16 malignant at stage III-IV and 50 benign). Six patients with ovarian carcinoma were monitored during the first week after surgery and chemiotherapy for a total of 150 days of treatment. It has been observed that CA-125 serum level is consistently above the normal range (>35 U/ml) in all malignant diseases. In benign pathology, levels above the normal were found to be represented almost exclusively by ovarian endometriosis. Furthermore, the results demonstrate that chemiotherapy alone is capable of lowering CA-125 serum levels. This tumor marker may be of great advantage in diagnosis and follow-up of ovarian malignancy

  17. Soc.ca: Specific Correspondence Analysis for the Social Sciences

    DEFF Research Database (Denmark)

    2017-01-01

    Specific and class specific multiple correspondence analysis on survey-like data. Soc.ca is optimized to the needs of the social scientist and presents easily interpretable results in near publication ready quality.......Specific and class specific multiple correspondence analysis on survey-like data. Soc.ca is optimized to the needs of the social scientist and presents easily interpretable results in near publication ready quality....

  18. Secure electronic commerce communication system based on CA

    Science.gov (United States)

    Chen, Deyun; Zhang, Junfeng; Pei, Shujun

    2001-07-01

    In this paper, we introduce the situation of electronic commercial security, then we analyze the working process and security for SSL protocol. At last, we propose a secure electronic commerce communication system based on CA. The system provide secure services such as encryption, integer, peer authentication and non-repudiation for application layer communication software of browser clients' and web server. The system can implement automatic allocation and united management of key through setting up the CA in the network.

  19. Envejecimiento Neuronal y Transporte de Ca2+ Intracelular

    OpenAIRE

    Hernando Pérez, Mª Elena

    2017-01-01

    El envejecimiento promueve pérdida cognitiva y susceptibilidad a enfermedades neurodegenerativas, lo que se ha relacionado con la dishomeostasis del Ca2+ intracelular. Para investigar esta hipótesis se utiliza el cultivo a largo plazo de neuronas de hipocampo de rata, modelo de envejecimiento neuronal in vitro. Mediante imagen de fluorescencia se han estudiado cambios en transporte de Ca2+ en neuronas a lo largo del envejecimiento in vitro. Las neuronas se han identificado mediante inmunofluo...

  20. PIK3CA alterations in Middle Eastern ovarian cancers

    Directory of Open Access Journals (Sweden)

    Uddin Shahab

    2009-07-01

    Full Text Available Abstract Background PI3K/AKTsignaling pathway plays an important role in cell growth, proliferation, and tumorgenesis of various malignancies. This signaling pathway has been shown to be frequently altered in several human cancers including ovarian cancers. However the role of this oncogenic signaling pathway has not been explored in the Middle Eastern epithelial ovarian cancer (EOC. Therefore, we investigated PI3K/AKT genetic alterations such as PIK3CA amplification, PIK3CA mutation, PTEN protein loss and their relationships with various clinicopathological characteristics in 156 EOCs. Results Fluorescence in situ hybridization (FISH technique and DNA sequencing were used to analyze PIK3CA amplification and mutation respectively. Expression of PIK3CA protein expression (p110 α, PTEN, p-AKT and Ki-67 was analyzed by immunohistochemistry. PIK3CA amplification was seen in 54 of 152 (35.5% EOC cases analyzed; PIK3CA gene mutations in 6/153 EOC (3.9%; KRAS mutations in 3/154 EOC (1.9%, BRAF mutations in 3/156 EOC (1.9%, p53 mutation in 50/154 EOC (32.5%, and loss of PTEN protein expression in 33/144 EOC (22.9%. p110 α overexpression was associated with increased phosphorylation of AKT-Ser 473 and with the proliferation marker Ki-67. Conclusion Our data showed mutual exclusivity between the molecular event of PIK3CA amplification and mutations in PIK3CA, KRAS, BRAF genes, which suggests that each of these alterations may individually be sufficient to drive ovarian tumor pathogenesis independently. High prevalence of genetic alterations in PI3K/AKT pathway in a Middle Eastern ovarian carcinoma provides genetic evidence supporting the notion that dysregulated PI3K/AKT pathways play an important role in the pathogenesis of ovarian cancers.