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  1. Stresses of Single Parenting

    Science.gov (United States)

    ... Text Size Email Print Share Stresses of Single Parenting Page Content Article Body What are some ways ... way. Check your local library for books on parenting. Local hospitals, the YMCA, and church groups often ...

  2. Perceptions of Parent School Collaboration within Single Parent Households

    Science.gov (United States)

    Josafat, Jason Marc

    2015-01-01

    Little is known regarding the involvement levels of single parents in their child's education or what schools can do to support the collaborative involvement with single parents. This is important, because parent involvement is crucial for student success, and schools play an important part in garnering this role towards parent involvement; single…

  3. Single Mother Parenting and Adolescent Psychopathology.

    Science.gov (United States)

    Daryanani, Issar; Hamilton, Jessica L; Abramson, Lyn Y; Alloy, Lauren B

    2016-10-01

    Children raised in single-mother families are at increased risk for psychopathology, but the mechanisms that help explain this relationship are understudied. In a community sample of diverse adolescents (N = 385, 52 % female, 48 % Caucasian) and their mothers, we hypothesized that single mothers would be more likely than cohabitating mothers to engage in negative parenting behaviors, which would predict adolescent psychopathology prospectively. Single mothers were more likely to engage in psychologically controlling behaviors, which predicted to their adolescent offspring experiencing higher rates of depressive symptoms and externalizing disorders. Girls were more susceptible to depressive symptoms via psychologically controlling parenting than boys in single-mother families. Further, single mothers were more likely to engage in rejecting parenting behaviors, which predicted to a higher prevalence of adolescent externalizing disorders. Surprisingly, rejection in single-mother families predicted to less severe anxiety symptoms in adolescents relative to two-parent families. It is likely that single mothers are not inherently inferior parents relative to cohabitating mothers; rather, their parenting practices are often compromised by a myriad of demands and stressors. Consistent with this postulate, low socioeconomic status was associated with single motherhood and negative parenting behaviors. Clinical implications and study limitations are discussed.

  4. Mental health among single and partnered parents in South Korea.

    Directory of Open Access Journals (Sweden)

    Kyoung Ae Kong

    Full Text Available This study compares the mental health of single parents relative to partnered parents and assesses the contribution of the social and demographic factors to this difference, examining the gender difference in it.We analyzed 12,024 single and partnered subjects, aged 30-59 years, living with children, aged 0-19 years, drawn from the 4th, 5th, and 6th Korea National Health and Nutrition Examination Survey (KNHANES dataset in South Korea conducted from 2007-2013. Mental health was evaluated by self-reported questionnaires including depressive mood for recent two weeks, presence of suicidal ideation, and the Korean version of the Alcohol Use Disorder Identification Test. Covariates included age, physical illness, socioeconomic status (family income, recipient of national basic livelihood guarantees, educational level, house ownership, job, and residential area, family structure, and support (co-residence of another adult. Multiple logistic regression was carried out and the explained fractions of each covariate was calculated.Single parents had significantly poorer mental health than their partnered counterparts, with odds ratio (OR of 2.02 (95% confidence interval (CI 1.56-2.63 for depressive symptoms, 1.69 (95% CI 1.27-2.25 for suicidal ideation, and 1.74 (95% CI 1.38-2.20 for any of the three mental health statuses (suspicious depression, suicidal ideation, and alcohol dependence after controlling for the covariates. The odds of depressive symptoms (OR = 3.13, 95% CI 2.50-3.93 and suicidal ideation (OR = 2.50, 95% CI 1.97-3.17 among both single fathers and mothers were higher than partnered parents. However, the odds of alcohol dependence were 3.6 times higher among single mothers than partnered mothers (OR = 3.58, 95% CI 1.81-7.08 and were 1.4 times greater among single fathers than partnered fathers (OR = 1.35, 95% CI 0.81-2.25. Socio-economic status explained more than 50% (except for substance use disorders of the poorer mental health in

  5. The diurnal salivary cortisol pattern of adolescents from families with single, ill and healthy parents

    NARCIS (Netherlands)

    Sieh, D.S.; Visser-Meily, J.M.A.; Oort, F.J.; Meijer, A.M.

    2012-01-01

    Objective: Adolescents of single and/or chronically ill parents (target groups) reportedly have elevated psychological stress. However, their salivary cortisol pattern as part of the physiological stress system has not been compared to controls. The aim of this study is to examine differential

  6. The diurnal salivary cortisol pattern of adolescents from families with single, ill and healthy parents

    NARCIS (Netherlands)

    Sieh, Dominik S.; Visser-Meily, Johanna M. A.; Oort, Frans J.; Meijer, Anne Marie

    2012-01-01

    Adolescents of single and/or chronically ill parents (target groups) reportedly have elevated psychological stress. However, their salivary cortisol pattern as part of the physiological stress system has not been compared to controls. The aim of this study is to examine differential outcomes in the

  7. Supporting children after single-incident trauma: parents'views.

    NARCIS (Netherlands)

    Alisic, E.; Boeije, H.R.; Jongmans, M.J.; Kleber, R.J.

    2012-01-01

    Objective. To strengthen trauma-informed health care by exploring parents’ experiences of assisting their child after single-incident trauma (eg, violence, accidents, and sudden loss). Method. Semistructured interviews with parents (N = 33) of 25 exposed children (8-12 years). Results. Responsive

  8. Treating the Changing Single Parent Family: A Return to Boundaries.

    Science.gov (United States)

    Greif, Geoffrey L.

    1996-01-01

    Examines the concept of boundaries--drawing of emotional lines between family subsystems, and between "family" and the outside world--in assessment and intervention with single parent families. Argues that clear, flexible boundaries are needed for healthy family functioning; that coalitions, rigid or unclear boundaries, and triangulation…

  9. Immune overload: Parental attitudes toward combination and single antigen vaccines.

    Science.gov (United States)

    Hulsey, Ella; Bland, Tami

    2015-05-21

    Parental concerns have led to a recent decline in immunization coverage, resulting in outbreaks of diseases that were once under control in the US. As the CDC vaccination schedule continues to increase in complexity, the number of required injections per office visit increases as well. Some parents perceive that there is trauma associated with the administration of multiple injections, and research shows that having multiple vaccines due in a single visit is associated with delays and lower immunization rates. Combination vaccines make vaccination more efficient by incorporating the antigens of several different diseases into a single injection, but many parents worry that they may overload the child's developing immune system and leave him or her susceptible to secondary infections. This literature review synthesizes current evidence regarding the parental fear of vaccine-induced immune system overload and the fear of vaccine-associated trauma, in an attempt to understand the scope and nature of these fears. Despite the wealth of knowledge about each of these fears individually, it is still unknown which is of greater concern and how this affects parental decision-making. Copyright © 2015 Elsevier Ltd. All rights reserved.

  10. Single parent households and increased child asthma morbidity.

    Science.gov (United States)

    Moncrief, Terri; Beck, Andrew F; Simmons, Jeffrey M; Huang, Bin; Kahn, Robert S

    2014-04-01

    To characterize whether single parent households are associated with pediatric asthma-related repeat healthcare utilization and to examine family-level psychosocial variables that may explain this relationship. We analyzed a prospective cohort of 526 children aged 1-16 years hospitalized for asthma or bronchodilator-responsive wheezing whose caregivers self-reported their marital status. Those reporting being "single" were considered the at-risk category. The outcome was repeat asthma-related utilization (emergency room (ER) revisit or hospital readmission) within 12 months. We assessed, a priori, four psychosocial variables (household income, caregiver risk of psychological distress, ratio of in-home children to adults, and regular attendance at childcare or a secondary home). Among all children enrolled in the cohort, 40% returned to the ER or hospital for asthma within 12 months. Of all caregivers, 59% self-identified as single. Single status was significantly associated with each psychosocial variable. Children in households with lower incomes and higher ratios of children to adults were both more likely to return to the ER or hospital than children with higher incomes and lower ratios, respectively (each p asthma from single parent households were more likely to have asthma-related reutilization within 12 months than children from homes with married parents. This was driven, in large part, by underlying differences in household income.

  11. The Need to Change Army Policies Toward Single Parents and Dual Military Couples With Children

    National Research Council Canada - National Science Library

    Carroll, Carolyn

    2005-01-01

    ... and to best manage our single parent service members and dual-military couples. There is a need to address the Army regulations that are ambiguous and contradictory toward enlisting and retaining single parents...

  12. The Circle Of Security Parenting and parental conflict: a single case study

    Directory of Open Access Journals (Sweden)

    Chiara ePazzagli

    2014-08-01

    Full Text Available The Circle of Security Parenting (COS-P is an early attachment based intervention that can be used with groups, dyads, and individuals. Created in the USA and now used in many countries, COS-P is a visually based approach that demonstrates its central principles through videos of parent/child interactions. The core purpose of the COS-P is to provide an opportunity for caregivers to reflect on their child’s needs and on the challenges each parent faces in meeting those needs. Even though there is a wide range of clinical settings in which child/parent attachment is an important component of assessment there is limited empirical data on when and how attachment based interventions are appropriate for specific clinical profiles and contexts. The aim of this paper is to present a clinical application of COS-P in order to explore and reflect on some specific therapeutic tasks where it works and on some clinical indicators and contexts appropriate for its application. A single case study of a father, M. (43 yrs. old in conflict for the custody of his 5yrs old daughter is reported. The Adult Attachment Projective Picture System (AAP, the Parenting Stress Index, the Strengths and Difficulties Questionnaire, and the Parental Alliance Measure, were administered pre- and post-intervention. The clinical significance analysis method revealed that numerous changes occurred in the father. The AAP showed improvements in the level of agency of self. M. made gains in his capacity to use internal resources and to increase his agency of self. M. was classified as recovered in his perception of the child’s functioning and as improved in his parenting stress and parenting alliance with the mother. Considerations on specific contexts and clinical indicators for the application of COS-P are proposed.

  13. Single Parenting and Child Behavior Problems in Kindergarten.

    Science.gov (United States)

    Jackson, Aurora P; Preston, Kathleen S J; Franke, Todd M

    2010-03-01

    Two waves of data from a sample of 89 poor and near-poor single black mothers and their preschool children were used to study the influences of parenting stress, physical discipline practices, and nonresident fathers' relations with their children on behavior problems in kindergarten. The results indicate that higher levels of parent stress, more frequent spanking, and less frequent father-child contact at time 1 were associated with increased teacher-reported behavior problems at time 2. In addition, more frequent contact between nonresident biological fathers and their children moderated the negative effect of harsh discipline by mothers on subsequent child behavior problems. Specifically, when contact with the father was low, maternal spanking resulted in elevated levels of behavior problems; with average contact, this negative effect of spanking was muted; and with high contact, spanking was not associated with increased behavior problems in kindergarten. The implications of these findings for future research and policy are discussed.

  14. 45 CFR 261.35 - Are there any special work provisions for single custodial parents?

    Science.gov (United States)

    2010-10-01

    ... custodial parents? 261.35 Section 261.35 Public Welfare Regulations Relating to Public Welfare OFFICE OF...? § 261.35 Are there any special work provisions for single custodial parents? Yes. A single custodial parent or caretaker relative with a child under age six will count as engaged in work if he or she...

  15. The Mental Health Status of Single-Parent Community College Students in California.

    Science.gov (United States)

    Shenoy, Divya P; Lee, Christine; Trieu, Sang Leng

    2016-01-01

    Single-parenting students face unique challenges that may adversely affect their mental health, which have not been explored in community college settings. The authors conducted secondary analysis of Spring 2013 data from the American College Health Association-National College Health Assessment to examine difficulties facing single-parent community college students and the association between single parenting and negative mental health (depression, self-injury, suicide attempt). Participants were 6,832 California community college students, of whom 309 were single parents. Demographic and mental health data were characterized using univariate descriptive analyses. Bivariate analyses determined whether single parents differed from other students regarding negative mental health or traumatic/difficult events. Finances, family, and relationship difficulties disproportionally affected single parents, who reported nearly twice as many suicide attempts as their counterparts (5.3% vs. 2.7%; p students face a higher prevalence of mental health stressors than other community college students.

  16. Single pion electro- and neutrino production on heavy targets

    International Nuclear Information System (INIS)

    Paschos, E. A.; Schienbein, I.; Yu, J.Y.

    2007-04-01

    We present a calculation of single pion electroproduction cross sections on heavy targets in the kinematic region of the Δ(1232) resonance. Final state interactions of the pions are taken into account using the pion multiple scattering model of Adler, Nussinov and Paschos (ANP model). For electroproduction and neutral current reactions we obtain results for carbon, oxygen, argon and iron targets and find a significant reduction of the W-spectra for π 0 as compared to the free nucleon case. On the other hand, the charged pion spectra are only little affected by final state interactions. Measurements of such cross sections with the CLAS detector at JLAB could help to improve our understanding of pion rescattering effects and serve as important/valuable input for calculations of single pion neutrino production on heavy targets relevant for current and future long baseline neutrino experiments. Two ratios, in Eq. (3.8) and (3.10), will test important properties of the model. (authors)

  17. Target Localization with a Single Antenna via Directional Multipath Exploitation

    Directory of Open Access Journals (Sweden)

    Ali H. Muqaibel

    2015-01-01

    Full Text Available Target localization in urban sensing can benefit from angle dependency of the pulse shape at a radar receiver antenna. We propose a localization approach that utilizes the embedded directivity in ultra-wideband (UWB antennas to estimate target positions. A single radar unit sensing operation of indoor targets surrounded by interior walls is considered, where interior wall multipaths are exploited to provide target cross-range. This exploitation assumes resolvability of the multipath components, which is made possible by the virtue of using UWB radar signals. The proposed approach is most attractive when only few multipaths are detectable due to propagation obstructions or owing to low signal-to-noise ratios. Both simulated and experimental data are used to demonstrate the effectiveness of the proposed approach.

  18. 34 CFR 403.81 - How must funds be used under the Single Parents, Displaced Homemakers, and Single Pregnant Women...

    Science.gov (United States)

    2010-07-01

    ..., Displaced Homemakers, and Single Pregnant Women Program? 403.81 Section 403.81 Education Regulations of the... Secretary Assist Under the Basic Programs? Single Parents, Displaced Homemakers, and Single Pregnant Women... Pregnant Women Program? A State shall use funds reserved in accordance with § 403.180(b)(2)(i) for...

  19. Moving Target Information Extraction Based on Single Satellite Image

    Directory of Open Access Journals (Sweden)

    ZHAO Shihu

    2015-03-01

    Full Text Available The spatial and time variant effects in high resolution satellite push broom imaging are analyzed. A spatial and time variant imaging model is established. A moving target information extraction method is proposed based on a single satellite remote sensing image. The experiment computes two airplanes' flying speed using ZY-3 multispectral image and proves the validity of spatial and time variant model and moving information extracting method.

  20. [Comparison of the factors influencing children's self-esteem between two parent families and single parent families].

    Science.gov (United States)

    Sok, Sohyune R; Shin, Sung Hee

    2010-06-01

    This study was done to compare factors influencing children's self-esteem between two parent families and single parent families. The participants were 692 children aged 11 to 13 yr (388 in two parent families and 304 in single parent families) recruited from 20 community agencies and 5 elementary schools in Gyeonggi Province and Seoul City, South Korea. Data were collected from May to July, 2007 using a survey questionnaire containing items on self-esteem, internal control, problematic behavior, school record, family hardiness, parent-child communication and social support. The data were analyzed using SPSS 15.0 program and factors affecting children's self-esteem were analyzed by stepwise multiple regression. Scores for the study variables were significantly different between the two groups. The factors influencing children's self-esteem were also different according to family type. For two parent families, internal control, problematic behavior, school record, and parent-child communication significantly predicted the level of self-esteem (adjusted R(2)=.505, pself-esteem (adjusted R(2)=.444, pself-esteem.

  1. Dating and sexual behavior among single parents of young children in the United States.

    Science.gov (United States)

    Gray, Peter B; Garcia, Justin R; Crosier, Benjamin S; Fisher, Helen E

    2015-01-01

    Theory and research on partnered parents suggests trade-offs between parenting and sexuality, with those trade-offs most pronounced among mothers of young children. However, little research has focused on how a growing demographic of single parents negotiates dating and sexual activity. The current study drew upon a 2012 nationally representative sample of 5,481 single Americans 21 years of age and older, of whom 4.3% were parents of a child age five or younger. Dependent variables were sexual thoughts, frequency of sexual activity, number of sexual partners in the past year, dates during the previous three months, and whether one was actively seeking a relationship partner. Covariates included parental age, sex/gender, sexual orientation, education, and income. Using the entire sample of singles, we found no main effects of number (0, 1, 2+) of children aged five years and younger or number of children aged two years and younger on dating and sexual behavior variables. Next, using analyses restricted to single parents (n = 2,121), we found that single parents with a child aged five years or younger, adjusting for covariates, reported greater frequency of sexual activity and first dates but no differences in other outcomes compared with single parents of older children.

  2. More than Just Openness: Developing and Validating a Measure of Targeted Parent-Child Communication about Alcohol

    OpenAIRE

    Miller-Day, Michelle; Kam, Jennifer A.

    2010-01-01

    Research addressing parent-child communication on the topic of alcohol use relies heavily on assessing frequency of discussions and general assessments of openness in parent-child communication, ignoring the complexity of this communication phenomenon. This study adds to the literature by articulating a conceptualization and developing a measurement of parent-child communication—targeted parent-child communication about alcohol—and comparing the efficacy of targeted parent-child communication...

  3. Smoke-free homes among single-parent families: Differences associated with parental race/ethnicity and smoking behaviors

    Directory of Open Access Journals (Sweden)

    Yujiao Mai

    2018-03-01

    Full Text Available We assessed differences in the rates of smoke-free homes among single-parent households with regard to parental race/ethnicity and smoking status. We identified two cohorts representative of the U.S. single-parent households with underage children (children under the age of 18 based on the Tobacco Use Supplement to the Current Population Survey: 2010–11 (n = 6474 and 2014–15 (n = 6114. The interviews were conducted by phone and in-person. Statistical analysis was performed in 2017. The overall rate of smoke-free homes was 82% in 2010–11 and 86% in 2014–15. The rate of a smoke-free home was highest for Non-Hispanic (NH Asian (94% and Hispanic (92% parents and lowest for NH Multiracial (77% in 2010–11 and 82% in 2014–15 in both survey periods. However, 2014–15 model-based comparisons relative to NH Whites indicated only one significant difference: the rate was lower for NH Blacks (OR = 0.46, 99% CI = 0.32:0.66. The smoke-free homes were least prevalent among daily smokers, followed by occasional smokers, followed by former smokers, and most prevalent among never smokers in each survey period. The 2010–11 and 2014–15 rates were 45% and 54% for daily, 64% and 72% for occasional, 89% and 91% for former, and 93% and 94% for never smokers. The gap in the rates of smoke-free homes for diverse parental racial/ethnic groups observed in 2010–11 decreased by 2014–15. While smoke-free homes became more prevalent in 2014–15, the rates remain drastically different among families with different parental smoking behaviors. Exposure to secondhand smoke at home remains common among single-parent households where the parent smokes.

  4. High power laser interaction with single and double layer targets

    Czech Academy of Sciences Publication Activity Database

    Borodziuk, S.; Demchenko, N. N.; Gus'kov, S. Yu.; Jungwirth, Karel; Kálal, M.; Kasperczuk, A.; Kondrashov, V. N.; Králiková, Božena; Krouský, Eduard; Limpouch, Jiří; Mašek, Karel; Pisarczyk, P.; Pisarczyk, T.; Pfeifer, Miroslav; Rohlena, Karel; Rozanov, V. B.; Skála, Jiří; Ullschmied, Jiří

    2005-01-01

    Roč. 35, č. 2 (2005), s. 241-262 ISSN 0078-5466 R&D Projects: GA MŠk(CZ) LN00A100; GA AV ČR(CZ) KSK2043105 Grant - others:EU(XE) HPRI-CT-1999-00053; RFBR(RU) 02-02-16966; IAEA(XE) 11655/RBF; INTAS(XX) 01-0572 Institutional research plan: CEZ:AV0Z10100523; CEZ:AV0Z20430508 Keywords : laser produced plasma * three-frame interferometry * macroparticle * single and double targets * crater * shock wave * laser energy absorption Subject RIV: BH - Optics, Masers, Lasers Impact factor: 0.459, year: 2005

  5. [Being raised by lesbian parents or in a single-parent family is no risk factor for problem behavior, however being raised as an adopted child is].

    Science.gov (United States)

    Verhulst, F C; Versluis-den Bieman, H O; Balmus, N C

    1997-03-01

    Modern reproductive techniques and alternative family structures (with single or homosexual parents and adoption situations) raise questions about the consequences for the growing children involved. Genetic links appear to be less important for the functioning of a family than a strong wish for parenthood; parents who have become parents only through great efforts display a better quality of parenthood than average natural parents. Characteristics of the parent/parents, such as paedagogic qualities, and the quality of the parent-child relationship appear more important than the type of family. Published results of research reveal no reason why lesbian families should be judged differently from heterosexual ones as family types for the raising of children. The main negative factor for the functioning of the child growing up in a single-parent family is the marriage conflicts that have led to the single-parent situation; being raised by a single parent in itself has no adverse effect. Raising adopted children from other countries makes far greater demands on the adoptive parents than parents of biological children have to meet. The raising of a foreign adopted child by a single parent entails additional risks for the child's development. Data on the development of children in alternative family structures frequently concern exceptionally competent parents, which may have biased the findings.

  6. Adolescents’ Self-Esteem in Single and Two-Parent Families

    OpenAIRE

    Alami, Ali; Khosravan, Shahla; Sadegh Moghadam, Leila; Pakravan, Fateme; Hosseni, Fateme

    2014-01-01

    Background: Self-esteem is one of the basic needs for all individuals especially in adolescence. The aim of this study was to determine associations between adolescents’ self-esteem and perceived maternal parenting styles as well as its dimensions in terms of family type. Methods: In this analytic cross-sectional study, 356 high school students (250 two-parent nuclear family and 106 single-parent family) participated and filled out the Coppersmith self-esteem and the Robinson and colleagues (...

  7. Single Custodial Fathers' Involvement and Parenting: Implications for Outcomes in Emerging Adulthood

    Science.gov (United States)

    Bronte-Tinkew, Jacinta; Scott, Mindy E.; Lilja, Emily

    2010-01-01

    Using a sample of 3,977 youths from the National Longitudinal Survey of Youth (NLSY97), this study examines the unique characteristics of single-custodial-father families with adolescents and the effects of single fathers' involvement and parenting on outcomes in emerging adulthood. Findings suggest that single-custodial-father families are…

  8. Single session email consultation for parents : An evaluation of its effect on empowerment

    NARCIS (Netherlands)

    prof.dr. Jo Hermanns; prof.dr. Ruben Fukkink; dr. Christa C.C. Nieuwboer

    2014-01-01

    This study evaluated the effect of single session email consultation on empowerment of parents. Practitioners in a control group (N = 19) received no training; practitioners in an experimental group (N = 21) were trained to use empowerment oriented techniques in online consultation. Parental

  9. Single Session Email Consultation for Parents: An Evaluation of Its Effect on Empowerment

    Science.gov (United States)

    Nieuwboer, Christa C.; Fukkink, Ruben G.; Hermanns, Jo M. A.

    2015-01-01

    This study evaluated the effect of single session email consultation (SSEC) on empowerment of parents. Practitioners in a control group (n = 19) received no training and practitioners in an experimental group (n = 21) were trained to use empowerment-oriented techniques in online consultation. Parental empowerment was measured (n = 96) through a…

  10. The Need to Change Army Policies Toward Single Parents and Dual Military Couples With Children

    National Research Council Canada - National Science Library

    Carroll, Carolyn

    2005-01-01

    The purpose of this report is to identify the key challenges with current Army personnel policies and to provide recommendations that would reduce the impact of single parents on Army unit readiness...

  11. Caregiving, single parents and cumulative stresses when caring for a child with cancer.

    Science.gov (United States)

    Granek, L; Rosenberg-Yunger, Z R S; Dix, D; Klaassen, R J; Sung, L; Cairney, J; Klassen, A F

    2014-03-01

    Single parents whose children have cancer are a marginalized group who report less family centred care, and therefore, less quality cancer care for their children. As such, the aims of this study were to explore how single parents of children with cancer describe their caregiving experiences and to understand their contextual life stressors. A constructivist grounded theory method was used. Qualitative interviews with 29 single parents of children with cancer who were at least 6 months post-diagnosis were recruited between November 2009 and April 2011 from four hospitals across Canada. Line-by-line coding was used to establish codes and themes and constant comparison was used to establish relationships among emerging codes and conceptual themes. The first set of findings report on caregiving duties including: emotional tasks, informational tasks and physical tasks. The second set of findings report on the contextual picture of parent's lives including their living conditions, their physical and mental health and their family histories of disruption, trauma and disease. Single parents caring for children with cancer were found to experience several cumulative stressors in addition to the current strain of caring for a child with cancer. The synergy of these cumulative stresses with the added strain of caregiving for a child with cancer may have long-term health and financial implications for parents. Broad-based policy interventions should focus on relieving the chronic strains associated with being a single parent of a child with cancer. © 2012 John Wiley & Sons Ltd.

  12. Adolescents' self-esteem in single and two-parent families.

    Science.gov (United States)

    Alami, Ali; Khosravan, Shahla; Sadegh Moghadam, Leila; Pakravan, Fateme; Hosseni, Fateme

    2014-04-01

    Self-esteem is one of the basic needs for all individuals especially in adolescence. The aim of this study was to determine associations between adolescents' self-esteem and perceived maternal parenting styles as well as its dimensions in terms of family type. In this analytic cross-sectional study, 356 high school students (250 two-parent nuclear family and 106 single-parent family) participated and filled out the Coppersmith self-esteem and the Robinson and colleagues (2001) perceived parenting styles questionnaires. Data were analyzed by SPSS software, version 18. To assess the relationship between participants' self-esteem and parenting styles and dimensions, Mantel-Haenszel Chi-square test was used to adjust the effect of potential confounder variables. P≤0.05 was considered statistically significant. From a total of 370 questionnaires, 356 questionnaires were completed. The mean±SD of the participants' self-esteem score was 38.49±6.55. Mean±SD of self-esteem score among the two-parent and single-parent students was 39.06±6.36 and 37.42±7.28, respectively (P=0.034). Dominant parenting style in both families was authoritative style. There were significant associations between the respondents' self-esteem and their perceived parenting styles, after matching sex, family income, level of education, and parents job (Pself-esteem. Therefore, considering the relationship between child-rearing style and adolescent self-esteem, assessing other relating factors with adolescent self-esteem especially in single-parent family, such as father absence stigma, is suggested.

  13. Adolescents’ Self-Esteem in Single and Two-Parent Families

    Science.gov (United States)

    Alami, Ali; Khosravan, Shahla; Sadegh Moghadam, Leila; Pakravan, Fateme; Hosseni, Fateme

    2014-01-01

    Background: Self-esteem is one of the basic needs for all individuals especially in adolescence. The aim of this study was to determine associations between adolescents’ self-esteem and perceived maternal parenting styles as well as its dimensions in terms of family type. Methods: In this analytic cross-sectional study, 356 high school students (250 two-parent nuclear family and 106 single-parent family) participated and filled out the Coppersmith self-esteem and the Robinson and colleagues (2001) perceived parenting styles questionnaires. Data were analyzed by SPSS software, version 18. To assess the relationship between participants’ self-esteem and parenting styles and dimensions, Mantel–Haenszel Chi-square test was used to adjust the effect of potential confounder variables. P≤0.05 was considered statistically significant. Results: From a total of 370 questionnaires, 356 questionnaires were completed. The mean±SD of the participants’ self-esteem score was 38.49±6.55. Mean±SD of self-esteem score among the two-parent and single-parent students was 39.06±6.36 and 37.42±7.28, respectively (P=0.034). Dominant parenting style in both families was authoritative style. There were significant associations between the respondents’ self-esteem and their perceived parenting styles, after matching sex, family income, level of education, and parents job (Pself-esteem. Therefore, considering the relationship between child-rearing style and adolescent self-esteem, assessing other relating factors with adolescent self-esteem especially in single-parent family, such as father absence stigma, is suggested. PMID:25349847

  14. 34 CFR 403.82 - In what settings may the Single Parents, Displaced Homemakers, and Single Pregnant Women Program...

    Science.gov (United States)

    2010-07-01

    ... EDUCATION STATE VOCATIONAL AND APPLIED TECHNOLOGY EDUCATION PROGRAM What Kinds of Activities Does the... secondary school settings, including area vocational education schools, and community-based organizations... 34 Education 3 2010-07-01 2010-07-01 false In what settings may the Single Parents, Displaced...

  15. Parental Factors Associated with Child Post-traumatic Stress Following Injury: A Consideration of Intervention Targets

    Directory of Open Access Journals (Sweden)

    Anna E. Wise

    2017-08-01

    Full Text Available Post-traumatic stress disorder (PTSD symptoms are relatively common following pediatric traumatic injury and are related to poor long-term child outcomes. However, due to concerns regarding the efficacy of early child preventive interventions, and difficulty intervening with injured and medicated children soon after the event, it is not feasible to provide early psychological interventions to children exposed to traumatic injury. Parental PTSD symptoms and reactions to the child’s traumatic injury impact child outcomes and provide potential targets for early intervention to reduce child symptom development without involving the child. The authors conducted a review of the literature using Psycinfo and Pubmed research databases (publication years = 1990–2017 and identified 65 published studies relevant to the topic of the review. The present review considers parent factors [parenting styles, parental post-traumatic pathology (PTS, adaptive and maladaptive coping strategies, and communication regarding the traumatic injury] and their impact on child PTS. We focus specifically on factors amenable to intervention. We further review moderators of these relationships (e.g., child age and gender, parent gender and conclude that it is unlikely that a one-size-fits-all approach to treatment will be successful. Rather, it is necessary to consider the age and gender of parent child dyads in designing and providing targeted interventions to families following the traumatic injury of a child.

  16. Single-parent households and children's educational achievement: A state-level analysis.

    Science.gov (United States)

    Amato, Paul R; Patterson, Sarah; Beattie, Brett

    2015-09-01

    Although many studies have examined associations between family structure and children's educational achievement at the individual level, few studies have considered how the increase in single-parent households may have affected children's educational achievement at the population level. We examined changes in the percentage of children living with single parents between 1990 and 2011 and state mathematics and reading scores on the National Assessment of Educational Progress. Regression models with state and year fixed effects revealed that changes in the percentage of children living with single parents were not associated with test scores. Increases in maternal education, however, were associated with improvements in children's test scores during this period. These results do not support the notion that increases in single parenthood have had serious consequences for U.S. children's school achievement. Copyright © 2015 Elsevier Inc. All rights reserved.

  17. Parenting

    Science.gov (United States)

    ... parents, people are always ready to offer advice. Parenting tips, parents' survival guides, dos, don'ts, shoulds ... right" way to be a good parent. Good parenting includes Keeping your child safe Showing affection and ...

  18. Analisis Pengaruh Jumlah Tanggungan Keluarga, Umur, Pendidikan Dan Status Pekerjaan Terhadap Pendapatan Keluarga Wanita Single Parent

    Directory of Open Access Journals (Sweden)

    Shabrina Umi Rahayu

    2015-11-01

    Full Text Available Women have significant roles in development, such as women as human resources in development, women as a subject in development, and women as a family builder. This study focused on how women’s role as a family builder in a single parent status. The variables used in this study are number of dependents, age, education, and employment status to be analyzed its impact on income of single parent women. This research took a place in Sesetan Village, South Denpasar with the number of samples taken were 84 samples with the purposive sampling method. The data was collected by questionnaires and interviews. Multiple linear regression analysis with quantitative and qualitative independent variables was used as the analysis technique. Based on the analysis result had found that variables of number of dependents, age, education, and employment status had a positive impact as a partial to the income of single parent women. The government’s efforts for all the kinds of women coaching are important to be developed, with the aim to the community prosperity especially single parent women who have heavier burden of life. The attention of education, health, and economy is necessary to improved to ease the burden for the families of the single parent women.

  19. Relationships between psychosocial factors and abusive parenting attitudes in low-income single mothers.

    Science.gov (United States)

    Lutenbacher, Melanie

    2002-01-01

    Linkages among family violence, maternal mental health, and parenting attitudes are not clearly understood. To investigate the relationships of abuse (childhood and/or partner), everyday stressors, self-esteem, depressive symptoms, and anger with abusive parenting attitudes. Cross-sectional analysis of data collected in interviews with 53 low-income, single mothers from wave two of a 3-wave study. A conceptual framework and bivariate correlations guided a series of multiple regressions to identify the best predictors for each variable. A high prevalence of abuse, depressive symptoms, and abusive parenting attitudes was found. Few women had ever received mental health treatment. Abuse (partner and childhood physical) predicted higher everyday stressors which in turn predicted lower self-esteem. Childhood abuse and lower self-esteem predicted more depressive symptoms. More depressive symptoms were related to higher levels of state anger. More everyday stressors and more depressive symptoms predicted higher levels of trait anger. Higher levels of anger expression were associated with higher everyday stressors and lower self-esteem. The presence of partner abuse best predicted higher levels of overall abusive parenting attitudes and more parent-child role reversal. Less parental empathy was associated with higher levels of state anger. This study partially explains the relationships of maternal abuse history and mental health status with parenting attitudes. Other predictors of parenting attitudes remain to be identified. The findings underscore the need for healthcare providers to consider the mental health status and abuse histories of low-income, single mothers. The potential disturbance in the parenting process of single mothers in abusive relationships warrants further investigation.

  20. More than just openness: developing and validating a measure of targeted parent-child communication about alcohol.

    Science.gov (United States)

    Miller-Day, Michelle; Kam, Jennifer A

    2010-06-01

    Research addressing parent-child communication on the topic of alcohol use relies heavily on assessing frequency of discussions and general assessments of openness in parent-child communication, ignoring the complexity of this communication phenomenon. This study adds to the literature by articulating a conceptualization and developing a measurement of parent-child communication-targeted parent-child communication about alcohol-and comparing the efficacy of targeted parent-child communication about alcohol in predicting positive expectancies of alcohol use and recent alcohol use. The predictive power of general openness in parent-child communication and frequency of communication about alcohol also were assessed. Students in fifth and sixth grade (N = 1,407) from 29 public schools completed surveys. Targeted parent-child communication about alcohol was negatively associated with both outcomes. Frequency and general openness were only negatively associated with positive expectancies regarding alcohol. Implications of these findings for the etiology and prevention of substance use are discussed.

  1. Social Aspects Regarding the Single-Parent Families Vulnerability - The Case Of Arad County

    Directory of Open Access Journals (Sweden)

    Marţian Iovan

    2016-07-01

    single-parent families must rely on the complete knowledge of this social phenomenon, with the need to establish databases and map the dispersion of single-parent families within administrative-territorial units. The decrease in the number of families with a high social risk over time is the result of correlated and harmonized public policies, aimed at fighting poverty, discrimination, unemployment, lack of access to education, while ensuring the general background for economic development and prosperity. The study is determined by the fact that many times the parents and the children of single parent families find themselves in the situation of not being able to make ends meet, the lack of finances, education and proper housing making it impossible for them to live a decent life. By identifying, through specific scientific methods such as document analysis, interviews, case studies, the particular types of problems facing single parent families, we consider we will succeed in offering a strong basis to motivate decision makers to establish additional social protection measures that will contribute to the reduction of the causes that maintain this social category among vulnerable groups.

  2. Health Characteristics of Solo Grandparent Caregivers and Single Parents: A Comparative Profile Using the Behavior Risk Factor Surveillance Survey

    Directory of Open Access Journals (Sweden)

    Deborah M. Whitley

    2015-01-01

    Full Text Available Objectives. To describe the health characteristics of solo grandparents raising grandchildren compared with single parents. Methods. Using the 2012 Behavioral Risk Factor Surveillance System, respondents identified as a single grandparent raising a grandchild were categorized as a solo grandparent; grandparent responses were compared with single parents. Descriptive analysis compared health characteristics of 925 solo grandparents with 7,786 single parents. Results. Compared to single parents, grandparents have a higher prevalence of physical health problems (e.g., arthritis. Both parent groups have a high prevalence of lifetime depression. A larger share of grandparents actively smoke and did no recreational physical exercise in the last month. However, grandparents appear to have better access to health services in comparison with single parents. Conclusion. Solo grandparents may be at risk for diminished physical capacity and heightened prevalence of depression. Health professionals can be an important resource to increase grandparents’ physical and emotional capacities.

  3. Health Characteristics of Solo Grandparent Caregivers and Single Parents: A Comparative Profile Using the Behavior Risk Factor Surveillance Survey

    Science.gov (United States)

    Whitley, Deborah M.

    2015-01-01

    Objectives. To describe the health characteristics of solo grandparents raising grandchildren compared with single parents. Methods. Using the 2012 Behavioral Risk Factor Surveillance System, respondents identified as a single grandparent raising a grandchild were categorized as a solo grandparent; grandparent responses were compared with single parents. Descriptive analysis compared health characteristics of 925 solo grandparents with 7,786 single parents. Results. Compared to single parents, grandparents have a higher prevalence of physical health problems (e.g., arthritis). Both parent groups have a high prevalence of lifetime depression. A larger share of grandparents actively smoke and did no recreational physical exercise in the last month. However, grandparents appear to have better access to health services in comparison with single parents. Conclusion. Solo grandparents may be at risk for diminished physical capacity and heightened prevalence of depression. Health professionals can be an important resource to increase grandparents' physical and emotional capacities. PMID:26448744

  4. Hubungan Work-Family Conflict Dengan Continuance Commitment Pada Wanita Yang Berperan Sebagai Single Parent

    OpenAIRE

    Tarigan, Selvia Veronika

    2014-01-01

    Being single parent for women will provide a variety of issues. The main problemis related tothe economic problems. To overcome the problems occurred woman who acts as a single parent, they must work and survive in the organization where she worked. Commitment to remain in the work referred to as continuance commitment. The decision to work and take care of the family can cause conflicts are referred to work-family conflict. This study was conducted to see the relationship between work-fa...

  5. Identifying Molecular Targets For PTSD Treatment Using Single Prolonged Stress

    Science.gov (United States)

    2014-10-01

    2012 Mindfulness and Self-compassion Meditation for Coping with Deployment-Related Posttraumatic...was the impact on technology transfer? Nothing to Report. d. What was the impact on society beyond science and technology? Nothing to Report. 5...Shalev) 04/1/2014 - 03/31/2019 Other support now closed Using the neuroscience of parental behavior to inform the science of

  6. Single parent status and children's objectively measured level of physical activity.

    Science.gov (United States)

    Singhammer, John; Ried-Larsen, Mathias; Møller, Niels Christian; Lund-Kristensen, Peter; Froberg, Karsten; Andersen, Lars Bo

    Single-parent family status has been investigated as a possible psychosocial determinant of children's level of physical activity (PA)-although with mixed and inconclusive results. Prevailing evidence of the importance of two-parent family status as a resource for children's PA is based on a mix of subjective and objective measurements of PA. Objectives: To investigate if the level of PA among children living with a single parent was lower compared to children living with two parents by means of a meta-analysis of published and unpublished studies. We restricted our analysis to studies with objective measurements of PA. Data sources: The databases, Social Science Citation Index, PsycINFO, PubMed, and EBSCO were searched (1987-2013). Study eligibility criteria: Observational studies comparing objectively measured PA between single-parent children and children from two-parent families. Study appraisal and synthesis methods: We used guidelines from the Cochrane Handbook of Systematic Reviews of Interventions and a modified version of the Newcastle-Ottawa Scale overall to assess the quality of the included studies. We refrained from calculation of summary scores. Twelve studies met the following inclusion criteria of which six were unpublished: (a) child age (6-18 years) and (b) objectively measured level of PA. Meta-analysis revealed pooled estimates of -0.01 for boys (95 % CI -0.04-0.03, p  = 0.77, I 2  = 6.5 %, p  = 0.38) and 0.01 for girls (95 % CI -0.03-0.04, p  = 0.62, I 2  = 21.0 %, p  = 0.24), respectively. Estimates show no differences in objectively measured physical activity between children living in single-parent families compared to children living with two parents. Analyses investigating seven potential moderators did not yield any statistical significant effect size estimates. No evidence of heterogeneity between studies was observed. Limitations: Retrieved articles were assessed by several of the authors. Blinding of the

  7. Comparison of Parenting Style in Single Child and Multiple Children Families

    Directory of Open Access Journals (Sweden)

    Masoumeh Alidosti

    2016-06-01

    Full Text Available Background and Purpose: Family is the first and the most important structure in human civilization in which social lifestyles, mutual understanding, and compatibility is learned. Studies have shown that parenting style, is one the most important and fundamental factors in personality development. The purpose of this study was comparison of parenting style in single child and multiple children families. Materials and Methods: This study, in total, 152 mothers from Andimeshk city, Iran, were selected by random sampling. Data were collected from a health-care center was chosen randomly, mothers who had 5-7 years old children were enrolled in this study. The data collecting tool was the questionnaire which investigates permissive, authoritative, and authoritarian parenting styles in parents. After data entry in SPSS software, the collected data were analyzed by ANOVA, independent t-test, and Pearson correlation test. Results: The mean age of the participants was 32.71 ± 5.39 years old participated in this study. 69 mothers (45.4% had one child, 53 (34.9% had 2 children, and 30 mothers (19.7% had 3 and more children. The mean score of permissive parenting style was 19.97 ± 5.13 in single child families; the mean score of authoritative (19.56 ± 4.70 and authoritarian parenting style (34.50 ± 2.81 that difference was significantly (P < 0.050. Conclusion: According to the results of this study, it seems that having more children would make parents more logical and paves the way for upbringing children. Therefore, it is recommended to plan some educational programs about this issue for parents.

  8. Low-Income, Single-Parent Francophone Mothers and the Educational Achievement of Their Children

    Science.gov (United States)

    Caissie, Julie; Gaudet, Jeanne d'Arc; Godin, Jeanne

    2017-01-01

    This phenomenological study focused on single-parent, low-income francophone mothers' relationships with the educational and cultural achievement of their children attending French-speaking schools in Anglo-dominant settings in New Brunswick (Canada). We conducted individual (N = 8) semi-structured interviews to solicit information about the…

  9. Testing the Efficacy of a Scholarship Program for Single Parent, Post-Freshmen, Full Time Undergraduates

    Science.gov (United States)

    Carpenter, Dick M., II; Kaka, Sarah J.; Tygret, Jennifer A.; Cathcart, Katy

    2018-01-01

    This study examines the efficacy of a scholarship program designed to assist single parent, post-freshmen, full time undergraduate students and predictors of success among a sample of said students, where success is defined as progress toward completion, academic achievement, and degree completion. Results of fixed effects regression and…

  10. A Longitudinal Study of Black Adoptions: Single Parent Transracial, and Traditional.

    Science.gov (United States)

    Shireman, Joan F.; Johnson, Penny R.

    1986-01-01

    This longitudinal study of Black children reared in single-parent, transracial, and traditional adoptive homes reveals that most children grow well in these homes. Interest at this reporting is chiefly focused on the transracially adopted children because their pattern of racial identity development differs from that of children in Black homes.…

  11. Are the Needs of Single Parents Serving in the Air Force Being Met?

    Directory of Open Access Journals (Sweden)

    Samantha E. Blanchard

    2012-03-01

    Full Text Available The military has taken extraordinary steps in establishing programs to support not only the member serving but their families as well. This article will examine military policy as it impacts single parents serving in the Air Force, highlighting existing programs, and calling for more research on this valuable population.

  12. URGENSI KELOMPOK SADAR HUKUM BAGI WANITA SINGLE PARENT; PENGALAMAN PENGABDIAN MASYARAKAT DI PESISIR SELATAN SUMATERA BARAT

    Directory of Open Access Journals (Sweden)

    Tiswarni Tiswarni

    2014-07-01

    Full Text Available The establishing of a group that has good awareness on law for single parent women in Batu Hampa, aims at facilitating knowledge and understanding on marriage problem and resolution from law perspective, both on divorce legalization and right-obligation as a single parent. An illegal divorce will take effect of her personal and social life such as citizen status or for a remarried action. The single parent  women  were  provided  with  a  law  counseling  in  order  to prepare them as a personal that has law awareness. They were given a partner community before the counseling. Partner group supported them to establish the group which has good law awareness. In case of a family which is about to have divorce, the groups will show their care and is hoped to give the better solution. They should be able to elaborate the knowledge and are able to help the others who have the same problem.  Keywords: single parent, aware of law group, community partnersCopyright © 2014 by Kafa`ah All right reservedDOI : 10.15548/jk.v4i2.99

  13. Gender differences in the mental health of single parents: New Zealand evidence from a household panel survey.

    Science.gov (United States)

    Collings, Sunny; Jenkin, Gabrielle; Carter, Kristie; Signal, Louise

    2014-05-01

    In many countries single parents report poorer mental health than partnered parents. This study investigates whether there are gender differences in the mental health of single parents in New Zealand (and whether any gender difference varies with that among partnered parents), and examines key social and demographic mediators that may account for this difference. We used data on 905 single parents and 4,860 partnered parents from a New Zealand household panel survey that included the Kessler-10 measure of psychological distress. Linear regression analyses were used to investigate both interactions of gender and parental status, and confounding or mediation by other covariates. High/very high levels of psychological distress were reported by 15.7 % of single mothers and 9.1 % of single fathers, and 6.1 % of partnered mothers and 4.1 % of partnered fathers. In an Ordinary Least Squares regression of continuous K10 scores on gender, parental status and the interaction of both (plus adjustment for ethnicity, number of children and age), female single parents had a 1.46 higher K10 score than male single parents (95 % CI 0.48-2.44; 1.46). This difference was 0.98 (95 % CI -0.04 to 1.99) points greater than the gender difference among partnered parents. After controlling for further confounding or mediating covariates (educational level, labour force status and socioeconomic deprivation) both the gender difference among single parents (0.38, -0.56 to 1.31) and the interaction of gender and parental status (0.28 greater gender difference among single parents, -0.69 to 1.65) greatly reduced in magnitude and became non-significant, mainly due to adjustment for individual socioeconomic deprivation. The poorer mental health of single parents remains an important epidemiological phenomenon. Although research has produced mixed findings of the nature of gender differences in the mental health of single parents, our research adds to the increasing evidence that it is single

  14. Housing Resources and Programs for Single Student Parents at Community and Technical Colleges. Fact Sheet #C396

    Science.gov (United States)

    Thorman, Abby; Otto, Jessica; Gunn-Wright, Rhiana

    2012-01-01

    Parents with dependent children now make up almost one in four students pursuing higher education in the United States (Miller, Gault, and Thorman 2011). Single parents face particular challenges pursuing higher education, including securing safe and affordable housing. Single mothers often must spend over half of their income on housing expenses,…

  15. Government Should Subsidize, Not Tax, Marriage: Social Policies Have Influenced the Rate of Growth in Single-Parent Families

    Science.gov (United States)

    Peterson, Paul E.

    2015-01-01

    Based upon reflections from the Moynihan report of 1965, this author notes that the root causes of the growth in single-parent families have yet to be well identified, making it difficult to figure out where to go next. However, from 1965 onward, social policies have influenced the rate of growth in single-parent families. What is needed is a…

  16. Motivational readiness of children to school in nuclear and single parent families

    Directory of Open Access Journals (Sweden)

    Kateryna Ostrovska

    2015-12-01

    Full Text Available The aim of the paper is a comparison of psychological readiness of the child to go to school in nuclear and single parent families. To obtain the objectives of the paper the following methods were used: 1 methods “Two schools” by L.A Venger to identify the level of formation of internal position of the student; 2 the method “Motivational research studies in older preschoolers” by M.R. Ginsburg; 3 method “Pattern” by L.I. Tsehanskaya to determine the degree of development of skills training activities; 4 method “Graphic dictation” by D. El’konin to study the ability to follow adult instructions. The investigated group consisted of 40 students from first grade secondary school - 20 students from nuclear families (12 girls and 8 boys and 20 students from single parent families (9 girls and 11 boys. As a result of qualitative, comparative and correlation analysis it was shown that readiness of children to go to school susbstantially depends on completness of their families. The children from families have a higher level of skill training and internal position than children from single parent families. This occurs because both parents pay more attention to the children in the forming of a willingness to learn in school. The studies have shown that in the group of children from nuclear families dominate the highest level of development of skills training activities, increased formation of internal positions and childrens social motivation. These indicators are the hallmarks of readiness to learn at school. Also, some recommendations to teachers are provided as for increase of motivation to learn in children from single parent families.

  17. Enhancing RGI lyase thermostability by targeted single point mutations

    DEFF Research Database (Denmark)

    Silva, Inês R.; Larsen, Dorte Møller; Jers, Carsten

    2013-01-01

    Rhamnogalacturonan I lyase (RGI lyase) (EC 4.2.2.-) catalyzes the cleavage of rhamnogalacturonan I in pectins by β-elimination. In this study the thermal stability of a RGI lyase (PL 11) originating from Bacillus licheniformis DSM 13/ATCC14580 was increased by a targeted protein engineering...

  18. Differential impact of a Dutch alcohol prevention program targeting adolescents and parents separately and simultaneously: low self-control and lenient parenting at baseline predict effectiveness.

    Science.gov (United States)

    Koning, Ina M; Verdurmen, Jacqueline E E; Engels, Rutger C M E; van den Eijnden, Regina J J M; Vollebergh, Wilma A M

    2012-06-01

    To test whether baseline levels of the factors accountable for the impact of the Prevention of Alcohol use in Students (PAS) intervention (self-control, perceived rules about alcohol and parental attitudes about alcohol), moderate the effect of the intervention. A cluster randomized trial including 3,490 Dutch early adolescents (M age=12.66, SD=.49) and their parents randomized over four conditions: 1) parent intervention, 2) student intervention, 3) combined intervention and 4) control group. Moderators at baseline were used to examine the differential effects of the interventions on onset of (heavy) weekly drinking at 34-month follow-up. The combined intervention was only effective in preventing weekly drinking among those adolescents who reported to have lower self-control and more lenient parents at baseline. No differential effect was found for the onset of heavy weekly drinking. No moderating roles of self-control and lenient parenting were found for the separate student and parent interventions regarding the onset of drinking. The combined intervention is more effective among adolescents with low-self control and lenient parents at baseline, both factors that were a specific target of the intervention. The relevance of targeting self-control in adolescents and restrictive parenting is underlined.

  19. Parental reports of behavioural outcome among paediatric leukaemia survivors in Malaysia: a single institution experience.

    Science.gov (United States)

    Hamidah, Alias; Sham Marina, Mohd; Tamil, Azmi M; Loh, C-Khai; Zarina, Latiff A; Jamal, Rahman; Tuti Iryani, Mohd Daud; Ratnam, Vijayalakshmi C

    2014-10-01

    To determine the behavioural impact of chemotherapy in survivors of acute lymphoblastic leukaemia (ALL) treated with chemotherapy only and to identify treatment-related or sociodemography-related factors that might be associated with behavioural outcome. We examined 57 survivors of childhood ALL, who were off treatment for at least 2 years and were in remission, aged 4-18 years, and 221 unrelated healthy controls. The Child Behaviour Checklist (CBCL) parent report was used either in English or in Bahasa Malaysia (the national language of Malaysia) to assess the behavioural outcome. Childhood ALL survivors had significantly higher scores on externalising behaviour on the CBCL parent report than did controls. Higher problem scores were found in ALL survivors with single parents on 'total problems' (P = 0.03) and subscales 'withdrawn' (P = 0.03), 'social problems' (P design the most appropriate remedy for problem behaviours detected in this multi-ethnic population. © 2014 John Wiley & Sons Ltd.

  20. Impact of target probability on single-trial EEG target detection in a difficult rapid serial visual presentation task.

    Science.gov (United States)

    Cecotti, Hubert; Sato-Reinhold, Joyce; Sy, Jocelyn L; Elliott, James C; Eckstein, Miguel P; Giesbrecht, Barry

    2011-01-01

    In non-invasive brain-computer interface (BCI), the analysis of event-related potentials (ERP) has typically focused on averaged trials, a current trend is to analyze single-trial evoked response individually with new approaches in pattern recognition and signal processing. Such single trial detection requires a robust response that can be detected in a variety task conditions. Here, we investigated the influence of target probability, a key factor known to influence the amplitude of the evoked response, on single trial target classification in a difficult rapid serial visual presentation (RSVP) task. Our classification approach for detecting target vs. non target responses, considers spatial filters obtained through the maximization of the signal to signal-plus-noise ratio, and then uses the resulting information as inputs to a Bayesian discriminant analysis. The method is evaluated across eight healthy subjects, on four probability conditions (P=0.05, 0.10, 0.25, 0.50). We show that the target probability has a statistically significant effect on both the behavioral performance and the target detection. The best mean area under the ROC curve is achieved with P=0.10, AUC=0.82. These results suggest that optimal performance of ERP detection in RSVP tasks is critically dependent on target probability.

  1. Using llama derived single domain antibodies to target botulinum neurotoxins

    Science.gov (United States)

    Swain, Marla D.; Anderson, George P.; Bernstein, Rachael D.; Liu, Jinny L.; Goldman, Ellen R.

    2010-04-01

    Llama serum contains both conventional IgG as well as unique forms of antibody that contain only heavy chains where antigen binding is mediated through a single variable domain. These variable domains can be expressed recombinantly and are referred to as single domain antibodies (sdAb). SdAb are among the smallest known naturally derived antigen binding fragments, possess good solubility, thermal stability, and can refold after heat and chemical denaturation. Llamas were immunized with either BoNT A or B toxoid and phage display libraries prepared. Single domain antibodies (sdAb) that were able to detect botulinum neurotoxin (BoNT) serotypes A and B were selected from their respective libraries. Here, the binders obtained by panning the BoNT B library on either BoNT B toxoid or BoNT B complex toxoid coated plates or BoNT B toxin coupled microspheres are described. Using these panning methods, we selected for binders that showed specificity for BoNT B. Phage displayed binders were screened, moved to a protein expression vector and soluble sdAb was produced. Using a Luminex flow cytometer binders were evaluated in direct binding assays. We have exploited the unique properties of sdAb and used them as biological recognition elements in immuno-based sensors that can detect BoNT B.

  2. Parenting African American Children With Autism: The Influence of Respect and Faith in Mother, Father, Single-, and Two-Parent Care.

    Science.gov (United States)

    Burkett, Karen; Morris, Edith; Anthony, Jean; Shambley-Ebron, Donna; Manning-Courtney, Patricia

    2016-08-10

    Parents are the most significant contributor to care of children with autism spectrum disorder (ASD), and as such research on African American parenting in ASD is conspicuously absent. Findings relevant to parenting are discussed from a study with urban African American families caring for children with ASD. An ethnonursing study was conducted with 24 African American family members of children with ASD and 28 professionals. Data were analyzed and reported as themes. Two universal themes of were found of respect and faith in God and family that influenced parental care. Two diverse themes of mother's watchful care and father's protective care, along with differences in feelings of isolation and dependence on supports were found among single- and two-parent families. When health care professionals increase their knowledge and understanding of cultural practices in the parental care of children with ASD, they provide health care that is culturally congruent. © The Author(s) 2016.

  3. The Need to Change Army Policies Toward Single Parents and Dual Military Couples With Children

    Science.gov (United States)

    2005-03-18

    SINGLE PARENT STATUS ACTIVE DUTY ENLISTED SOLDIERS ..8 TABLE 6. ACTIVE DUTY GENDER DISTRIBUTION BY GRADE ............................................ 10...493,563 (100%) TABLE 6. ACTIVE DUTY GENDER DISTRIBUTION BY GRADE WHAT THESE METRICS TELL US These data indicate that there is a problem in the Army...Army War College, March 1998) Fifth Amendment Sisters. "Pregnancy Disrimination ." Available from <http://userpages.aug.com /captbarb/pregnancy.html

  4. Duration and mutual entrainment of changes in parenting practices engendered by behavioral parent training targeting recently separated mothers.

    Science.gov (United States)

    Reed, Andrea; Snyder, James; Staats, Sarah; Forgatch, Marion S; Degarmo, David S; Patterson, Gerald R; Low, Sabina; Sinclair, Ryan; Schmidt, Nicole

    2013-06-01

    Parent management training (PMT) has beneficial effects on child and parent adjustment that last for 5 to 10 years. Short-term changes in parenting practices have been shown to mediate these effects, but the manner in which changes in specific components of parenting are sequenced and become reciprocally reinforcing (or mutually entrained) to engender and sustain the cascade of long-term beneficial effects resulting from PMT has received modest empirical attention. Long-term changes in parenting resulting from the Oregon model of PMT (PMTO) over a 2-year period were examined using data from the Oregon Divorce Study-II in which 238 recently separated mothers and their 6- to 10-year-old sons were randomly assigned to PMTO or a no treatment control (NTC) group. Multiple indicators of observed parenting practices were used to define constructs for positive parenting, monitoring and discipline at baseline, and at 6-, 12-, 18- and 30-months postbaseline. PMTO relative to NTC resulted in increased positive parenting and prevented deterioration in discipline and monitoring over the 30-month period. There were reliable sequential, transactional relationships among parenting practices; positive parenting supported better subsequent monitoring, and positive parenting and better monitoring supported subsequent effective discipline. Small improvements in parenting resulting from PMTO and small deteriorations in parenting in the NTC group may be sustained and amplified by mutually entrained relationships among parenting practices. These data about the change processes engendered by PMTO may provide information needed to enhance the power, effectiveness, and efficiency of behavioral parent training interventions.

  5. Effectiveness of Parental Skills Training on Worry, Anxiety and Self-Efficacy Beliefs of Single-Child and Multi-Child Parents

    Directory of Open Access Journals (Sweden)

    A Hajigholami Yazdi

    2013-06-01

    Full Text Available Introduction: Each family utilizes specific methods for personal and social education of their children. These methods that are called “Parenting style” are affected by various factors such as biological, cultural, social, political, and economic factors. The present study intends to investigate the effectiveness of parental skills training on worry, anxiety and self-efficacy beliefs of single-child and multi-child parents. Methods: In this experimental study, two private girls' school located in the city of Karaj, were randomly selected as the control and experimental groups. Parents of experimental group’s students (54 couples with a voluntary assignment participated in 8 training sessions. Data were obtained by General Self-efficacy Beliefs Questionnaire, Beck Anxiety Inventory (BAI, Penn State Worry Questionnaire (PSWQ which were then analyzed by t-test and ANOVA. Results: Results showed that there was not any significant difference in the pretest between single-child and multi-child parents. Regarding control and experimental groups, a significant difference has been detected between the pretest and posttest between two groups. Multifactor ANOVA test results also showed that the effect of parental skills training is significant on fear, anxiety and self-efficacy. But the number of children does not have any significant effect on the fear, anxiety and self-efficacy. Conclusion: Findings emphasize the necessity and importance of parental skills training to facilitate children nurture, decrease stress and worry resulting from parenting responsibility.

  6. Adult bone strength of children from single-parent families: the Midlife in the United States Study.

    Science.gov (United States)

    Crandall, C J; Karlamangla, A S; Merkin, S S; Binkley, N; Carr, D; Greendale, G A; Seeman, T E

    2015-03-01

    Bone health may be negatively impacted by childhood socio-environmental circumstances. We examined the independent associations of single-parent childhood and parental death or divorce in childhood with adult bone strength indices. Longer exposure to a single-parent household in childhood was associated with lower bone strength in adulthood. Because peak bone mass is acquired during childhood, bone health may be negatively impacted by childhood socio-environmental disadvantage. The goal of this study was to determine whether being raised in a single-parent household is associated with lower bone strength in adulthood. Using dual-energy X-ray absorptiometry data from 708 participants (mean age 57 years) in the Midlife in the United States Biomarker Project, we examined the independent associations of composite indices of femoral neck bone strength relative to load (in three failure modes: compression, bending, and impact) in adulthood with the experience of single-parent childhood and parental death or divorce in childhood. After adjustment for gender, race, menopause transition stage, age, and body mass index, each additional year of single-parent childhood was associated with 0.02 to 0.03 SD lower indices of adult femoral neck strength. In those with 9-16 years of single-parent childhood, the compression strength index was 0.41 SD lower, bending strength index was 0.31 SD lower, and impact strength index was 0.25 SD lower (all p values divorce during childhood was not by itself independently associated with adult bone strength indices. The magnitudes of these associations were unaltered by additional adjustment for lifestyle factors and socioeconomic status in childhood and adulthood. Independent of parental death or divorce, growing up in a single-parent household is associated with lower femoral neck bone strength in adulthood, and this association is not entirely explained by childhood or adult socioeconomic conditions or lifestyle choices.

  7. The model of children's social adjustment under the gender-roles absence in single-parent families.

    Science.gov (United States)

    Chen, I-Jun; Zhang, Hailun; Wei, Bingsi; Guo, Zeyao

    2018-01-14

    This study aimed to evaluate the effects of the gender-role types and child-rearing gender-role attitude of the single-parents, as well as their children's gender role traits and family socio-economic status, on social adjustment. We recruited 458 pairs of single parents and their children aged 8-18 by purposive sampling. The research tools included the Family Socio-economic Status Questionnaire, Sex Role Scales, Parental Child-rearing Gender-role Attitude Scale and Social Adjustment Scale. The results indicated: (a) single mothers' and their daughters' feminine traits were both higher than their masculine traits, and sons' masculine traits were higher than their feminine traits; the majority gender-role type of single parents and their children was androgyny; significant differences were found between children's gender-role types depending on different raiser, the proportion of girls' masculine traits raised by single fathers was significantly higher than those who were raised by single mothers; (b) family socio-economic status and single parents' gender-role types positively influenced parental child-rearing gender-role attitude, which in turn, influenced the children's gender traits, and further affected children's social adjustment. © 2018 International Union of Psychological Science.

  8. Male Heads of Family in Single-Parent Households: Men Adaptations to the Needs of Their Children

    Directory of Open Access Journals (Sweden)

    Andrés Mauricio Cano Rodas

    2016-01-01

    The experience of the head of a single-parent home has led interviewed parents to modify the exercise of their masculinity regarding communication, relationships, the manner to express affection and the everyday performance with their children, accepting the new family role by associating it with the responsibility of raising and caring for the children

  9. Age-Related Parenting Education: Model Development and Application to an Emerging Family Constellation - Single-by-Choice Mothers.

    Science.gov (United States)

    Holle, Kimberly Ann

    The purpose of this paper is twofold: (1) to describe an adaptation of the Schaefer Circumplex Model to age-related parenting theory and techniques, and (2) to illustrate its application in relation to the emerging numbers of single-by-choice mothers. The method described superimposes both a child's and a parent's cognitive and psychosocial…

  10. Single-cell analysis of targeted transcriptome predicts drug sensitivity of single cells within human myeloma tumors.

    Science.gov (United States)

    Mitra, A K; Mukherjee, U K; Harding, T; Jang, J S; Stessman, H; Li, Y; Abyzov, A; Jen, J; Kumar, S; Rajkumar, V; Van Ness, B

    2016-05-01

    Multiple myeloma (MM) is characterized by significant genetic diversity at subclonal levels that have a defining role in the heterogeneity of tumor progression, clinical aggressiveness and drug sensitivity. Although genome profiling studies have demonstrated heterogeneity in subclonal architecture that may ultimately lead to relapse, a gene expression-based prediction program that can identify, distinguish and quantify drug response in sub-populations within a bulk population of myeloma cells is lacking. In this study, we performed targeted transcriptome analysis on 528 pre-treatment single cells from 11 myeloma cell lines and 418 single cells from 8 drug-naïve MM patients, followed by intensive bioinformatics and statistical analysis for prediction of proteasome inhibitor sensitivity in individual cells. Using our previously reported drug response gene expression profile signature at the single-cell level, we developed an R Statistical analysis package available at https://github.com/bvnlabSCATTome, SCATTome (single-cell analysis of targeted transcriptome), that restructures the data obtained from Fluidigm single-cell quantitative real-time-PCR analysis run, filters missing data, performs scaling of filtered data, builds classification models and predicts drug response of individual cells based on targeted transcriptome using an assortment of machine learning methods. Application of SCATT should contribute to clinically relevant analysis of intratumor heterogeneity, and better inform drug choices based on subclonal cellular responses.

  11. Penetration of a Small Caliber Projectile into Single and Multi-layered Targets

    Directory of Open Access Journals (Sweden)

    Riad A.M.

    2010-06-01

    Full Text Available The normal penetration of armor-piercing projectiles into single and multi-layered steel plates has been investigated. An experimental program has been conducted to study the effect of spaced and in-contact layered targets on their ballistic resistance. Armor piercing projectiles with caliber of 7.62 mm were fired against a series of single and multi-layered steel targets. The projectile impact velocities were ranged from 300-600 m/s, whereas the total thicknesses of the tested single, spaced and in-contact layered steel targets were 3 mm. The penetration process of different tested target configurations has been simulated using Autodayn-2D hydrocode. The experimental measurements of the present work were used to discuss the effect of impact velocity, target configurations and number of layers of different spaced and in-contact layered steel targets on their ballistic resistance. In addition, the post-firing examination of the tested targets over the used impact velocity range showed that the single and each layer of spaced and in-contact laminated steel targets were failed by petalling. Finally, the obtained experimental measurements were compared with the corresponding numerical results of Autodyn-2D hydrocode, good agreement was generally obtained.

  12. Penetration of a Small Caliber Projectile into Single and Multi-layered Targets

    Science.gov (United States)

    Abdel-Wahed, M. A.; Salem, A. M.; Zidan, A. S.; Riad, A. M.

    2010-06-01

    The normal penetration of armor-piercing projectiles into single and multi-layered steel plates has been investigated. An experimental program has been conducted to study the effect of spaced and in-contact layered targets on their ballistic resistance. Armor piercing projectiles with caliber of 7.62 mm were fired against a series of single and multi-layered steel targets. The projectile impact velocities were ranged from 300-600 m/s, whereas the total thicknesses of the tested single, spaced and in-contact layered steel targets were 3 mm. The penetration process of different tested target configurations has been simulated using Autodayn-2D hydrocode. The experimental measurements of the present work were used to discuss the effect of impact velocity, target configurations and number of layers of different spaced and in-contact layered steel targets on their ballistic resistance. In addition, the post-firing examination of the tested targets over the used impact velocity range showed that the single and each layer of spaced and in-contact laminated steel targets were failed by petalling. Finally, the obtained experimental measurements were compared with the corresponding numerical results of Autodyn-2D hydrocode, good agreement was generally obtained.

  13. Abnormal Ventral and Dorsal Attention Network Activity During Single and Dual Target Detection in Schizophrenia

    Directory of Open Access Journals (Sweden)

    Amy M. Jimenez

    2016-03-01

    Full Text Available Early visual perception and attention are impaired in schizophrenia, and these deficits can be observed on target detection tasks. These tasks activate distinct ventral and dorsal brain networks which support stimulus-driven and goal-directed attention, respectively. We used single and dual target rapid serial visual presentation (RSVP tasks during fMRI with an ROI approach to examine regions within these networks associated with target detection and the attentional blink (AB in 21 schizophrenia outpatients and 25 healthy controls. In both tasks, letters were targets and numbers were distractors. For the dual target task, the second target (T2 was presented at 3 different lags after the first target (T1 (lag1=100ms, lag3=300ms, lag7=700ms. For both single and dual target tasks, patients identified fewer targets than controls. For the dual target task, both groups showed the expected AB effect with poorer performance at lag 3 than at lags 1 or 7, and there was no group by lag interaction. During the single target task, patients showed abnormally increased deactivation of the temporo-parietal junction (TPJ, a key region of the ventral network. When attention demands were increased during the dual target task, patients showed overactivation of the posterior intraparietal cortex, a key dorsal network region, along with failure to deactivate TPJ. Results suggest inefficient and faulty suppression of salience-oriented processing regions, resulting in increased sensitivity to stimuli in general, and difficulty distinguishing targets from non-targets.

  14. Target problem (mis) matching: predictors and consequences of parent-youth agreement in a sample of anxious youth.

    Science.gov (United States)

    Hoffman, Lauren J; Chu, Brian C

    2015-04-01

    Parents and youth often report discrepant target problems upon seeking treatment for youth psychopathology, which can have important impact on therapy processes (e.g., dropout) and treatment outcomes, as entry-level attitudes have been found to be influential in ultimate use and benefit of treatment. The current study examined parent-youth agreement within an anxiety disordered sample by assessing demographic and diagnostic factors that may predict matching, as well as the impact of matching on attrition, treatment outcome, and parental satisfaction. Ninety-five youth with principal anxiety disorders received cognitive-behavioral treatment for anxiety at a university outpatient clinic. Youth and parents independently identified target problems during the pretreatment assessment. Target problems were coded into 25 qualitative categories representing diagnostic, symptom, and functional impairment domains, including diffuse anxiety, social anxiety, academic achievement, oppositional/behavior problems, sleep problems, suicidal ideation, and family functioning. The majority of parent-youth dyads (67.4%) agreed on at least one target problem. Although problems related to diffuse anxiety and social anxiety were reported most frequently, relatively low rates of agreement were found in these domains. Kappa values demonstrated higher levels of agreement for problems with specific fears, school attendance, and panic and lower levels of agreement for difficulties with worry, shame, and self-esteem. Further, youth diagnosed with comorbid externalizing disorders were less likely to agree with their parents on at least one target problem. No effects were found for gender, age, or number of diagnoses in predicting agreement. Target problem agreement did not significantly impact rates of attrition or diagnostic remission, but did predict some measures of parental satisfaction. Results suggest that disagreement on treatment goals exists even within a narrow treatment population and

  15. Hope for children and families: targeting abusive parenting and the associated impairment of children.

    Science.gov (United States)

    Bentovim, Arnon; Elliott, Ian

    2014-01-01

    The purpose of this study is to distill the "effective practice elements" from randomised controlled interventions (RCTs) to prevent the recurrence of abusive and neglectful parenting and the associated health and developmental impairment of children. The resulting elements would be used then to develop a step-by-step modular-systemic approach to intervention that is suitable to the needs of a variety of frontline practitioners in social care, health, and education. A series of 22 randomised RCTs were analysed using the distillation and matching approach to establish the presence of effective practice elements. The focus was physical and sexual abuse, victims and children, and young people as perpetrators; neglect including failure to thrive, emotional abuse (exposure to violence and mental health issues). The studies were analysed for effective practice elements, across different approaches matched to interventions focused on parenting, on children and young people, and on family/professional relationships. The proportion of practice elements utilised in each form of maltreatment was defined. The distillation process resulted in a total of 47 practice elements present across all forms of maltreatment studied. An experienced group of practitioners from statutory and voluntary agencies ordered and integrated the most frequently utilised emerging elements into a series of step-by-step modules, which could fit the complex needs of families when maltreatment had occurred. The resulting manual, Hope for Children and Families, provides a "menu" of evidence-based, step-by-step modular interventions targeting the profile of abusive and neglectful parenting and associated impairments of children. To be effective for frontline practitioners, the manual will need to be delivered in a user-friendly format, training developed, and supervision and support provided.

  16. Biological aspects of the development and self-concept in adolescents living in single-parent families.

    OpenAIRE

    Veček, Andrea; Vidović, Vesna; Miličić, Jasna; Špoljar-Vržina, Sanja; Veček, Nenad; Arch-Veček, Branka

    2009-01-01

    In this study we investigate whether there are differences between adolescents who grow up in single-parent families and those who grow up in nucleus families. We have decided that there are no differences in the physical development between the adolescents who are growing up in single parent families and those growing up in nucleus families. There is no difference in the self-concept between these two groups, except in the ethical and moral self-image of adolescents living with one parent. A...

  17. Potential of Start Codon Targeted (SCoT) markers for DNA fingerprinting of newly synthesized tritordeums and their respective parents.

    Science.gov (United States)

    Cabo, Sandra; Ferreira, Luciana; Carvalho, Ana; Martins-Lopes, Paula; Martín, António; Lima-Brito, José Eduardo

    2014-08-01

    Hexaploid tritordeum (H(ch)H(ch)AABB; 2n = 42) results from the cross between Hordeum chilense (H(ch)H(ch); 2n = 14) and cultivated durum wheat (Triticum turgidum ssp. durum (AABB; 2n = 28). Morphologically, tritordeum resembles the wheat parent, showing promise for agriculture and wheat breeding. Start Codon Targeted (SCoT) polymorphism is a recently developed technique that generates gene-targeted markers. Thus, we considered it interesting to evaluate its potential for the DNA fingerprinting of newly synthesized hexaploid tritordeums and their respective parents. In this study, 60 SCoT primers were tested, and 18 and 19 of them revealed SCoT polymorphisms in the newly synthesized tritordeum lines HT27 and HT22, respectively, and their parents. An analysis of the presence/absence of bands among tritordeums and their parents revealed three types of polymorphic markers: (i) shared by tritordeums and one of their parents, (ii) exclusively amplified in tritordeums, and (iii) exclusively amplified in the parents. No polymorphism was detected among individuals of each parental species. Three SCoT markers were exclusively amplified in tritordeums of lines HT22 and HT27, being considered as polyploidization-induced rearrangements. About 70% of the SCoT markers of H. chilense origin were not transmitted to the allopolyploids of both lines, and most of the SCoTs scored in the newly synthesized allopolyploids originated from wheat, reinforcing the potential use of tritordeum as an alternative crop.

  18. [Controlling arachidonic acid metabolic network: from single- to multi-target inhibitors of key enzymes].

    Science.gov (United States)

    Liu, Ying; Chen, Zheng; Shang, Er-chang; Yang, Kun; Wei, Deng-guo; Zhou, Lu; Jiang, Xiao-lu; He, Chong; Lai, Lu-hua

    2009-03-01

    Inflammatory diseases are common medical conditions seen in disorders of human immune system. There is a great demand for anti-inflammatory drugs. There are major inflammatory mediators in arachidonic acid metabolic network. Several enzymes in this network have been used as key targets for the development of anti-inflammatory drugs. However, specific single-target inhibitors can not sufficiently control the network balance and may cause side effects at the same time. Most inflammation induced diseases come from the complicated coupling of inflammatory cascades involving multiple targets. In order to treat these complicated diseases, drugs that can intervene multi-targets at the same time attracted much attention. The goal of this review is mainly focused on the key enzymes in arachidonic acid metabolic network, such as phospholipase A2, cyclooxygenase, 5-lipoxygenase and eukotriene A4 hydrolase. Advance in single target and multi-targe inhibitors is summarized.

  19. Parents.

    Science.gov (United States)

    Hurst, Hunter, Ed.; And Others

    1986-01-01

    This document contains the fifth volume of "Today's Delinquent," an annual publication of the National Center for Juvenile Justice. This volume deals with the issue of the family and delinquency, examining the impact of parental behavior on the production of delinquent behavior. "Parents: Neglectful and Neglected" (Laurence D. Steinberg) posits…

  20. Psychosocial differences related to parenting infants among single and married mothers.

    Science.gov (United States)

    Copeland, Debra Beach; Harbaugh, Bonnie Lee

    2010-01-01

    This study compared the psychosocial factors, self-esteem, sense of mastery, life stress, and social support among first-time married and single mothers in early parenthood. A cross-sectional comparative design was used to study secondary data. A convenience sample of 80 first-time mothers completed self-report instruments on self-esteem, sense of mastery, social, and life stress at 6-8 weeks after birth. The data were analyzed using descriptive statistics, chi-square tests and ANOVA. A statistically significant difference was found between married and single mothers on self esteem (F = 9.314, df = 1, p = .003) and sense of mastery (F = 4.919, df = 1, p = .030). There were no statistical differences found between married and single mothers on social support and life stress. Findings support Belsky's (1984) theoretical assertion that personal psychological resources are most indicative of successful parenting. Further, interventions directed toward enhancing single mothers' sense of mastery and self-esteem may be needed to facilitate maternal role transition.

  1. Method of preparing and applying single stranded DNA probes to double stranded target DNAs in situ

    Science.gov (United States)

    Gray, J.W.; Pinkel, D.

    1991-07-02

    A method is provided for producing single stranded non-self-complementary nucleic acid probes, and for treating target DNA for use therewith. The probe is constructed by treating DNA with a restriction enzyme and an exonuclease to form template/primers for a DNA polymerase. The digested strand is resynthesized in the presence of labeled nucleoside triphosphate precursor. Labeled single stranded fragments are separated from the resynthesized fragments to form the probe. Target DNA is treated with the same restriction enzyme used to construct the probe, and is treated with an exonuclease before application of the probe. The method significantly increases the efficiency and specificity of hybridization mixtures by increasing effective probe concentration by eliminating self-hybridization between both probe and target DNAs, and by reducing the amount of target DNA available for mismatched hybridizations. No Drawings

  2. Effects of single-family rooms on nurse-parent and nurse-infant interaction in neonatal intensive care unit.

    Science.gov (United States)

    Toivonen, Mirka; Lehtonen, Liisa; Löyttyniemi, Eliisa; Axelin, Anna

    Single-family rooms in neonatal intensive care unit can provide longer interaction between family and staff. On the other hand, separation in private rooms has been shown detrimental to child development if parents are not present. To examine the effects of single-family rooms on nurse-family, nurse-parent and nurse-infant interaction time in neonatal intensive care unit. A quantitative, comparative, observational study was conducted before and after a move to a neonatal intensive care unit with single-family rooms. A total of 194 observation hours were conducted before the move and 194h after the move. The differences were analyzed using a hierarchical linear mixed model. Nurses working in one neonatal intensive care unit were recruited to study. The duration and number of nurse-parent and nurse-infant interaction episodes were recorded. The nurse-family and the nurse-parent interaction were longer in the unit with single-family rooms compared with the unit before the move (mean 261 vs. 138min per shift, pintensive care unit with single-family rooms supported an increase in nurse-parent interaction time. Importantly, nurse-infant interaction time did not decrease. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Strategies for public health initiatives targeting dairy consumption in young children: a qualitative formative investigation of parent perceptions.

    Science.gov (United States)

    Jung, Mary E; Bourne, Jessica E; Buchholz, Andrea; Martin Ginis, Kathleen A

    2017-11-01

    Dairy products contain essential nutrients to ensure healthy growth and bone development in children. However, a significant proportion of children in developed countries fail to consume the daily recommended intake of dairy products. Parents are the gatekeepers of familial nutritional intake and represent a potential vehicle through which to increase dairy consumption in children. As such, formative research was conducted to gain insight into parents' perceived barriers to and benefits of purchasing and consuming dairy products and to develop innovative message content that could be utilized in future public health campaigns. Seven in-depth group interviews were conducted in two phases between February and May 2015. Interviews were conducted in local recreational centres and libraries in British Columbia, Canada. Mothers (n 21, mean age 38 (sd 5) years) and fathers (n 9, mean age 38 (sd 3) years) of children aged 4-10 years. Parents perceived both positive and negative physical outcomes associated with consuming dairy. Lack of trustworthy information was a frequently discussed barrier theme to purchasing and consuming dairy products. Mothers were concerned about the cost of dairy products. Differences in purchasing and consumption strategies were reported between parents of children who consumed adequate dairy and those who did not. Parents believed the most appropriate communication channel was through print material. Messages targeting parents, as a means of increasing dairy consumption in children, should address barriers identified by parents. In addition, practical tips should be provided to promote purchasing and consumption of dairy products.

  4. Socioeconomic Status, Parenting, and Externalizing Problems in African American Single-Mother Homes: A Person-Oriented Approach

    Science.gov (United States)

    Anton, Margaret T.; Jones, Deborah J.; Youngstrom, Eric A.

    2016-01-01

    African American youth, particularly those from single-mother homes, are overrepresented in statistics on externalizing problems. The family is a central context in which to understand externalizing problems; however, reliance on variable-oriented approaches to the study of parenting, which originate from work with intact, middle-income, European American families, may obscure important information regarding variability in parenting styles among African American single mothers, and in turn, variability in youth outcomes as well. The current study demonstrated that within African American single-mother families: (a) a person-, rather than variable-, oriented approach to measuring parenting style may further elucidate variability; (b) socioeconomic status may provide 1 context within which to understanding variability in parenting style; and (c) 1 marker of socioeconomic status, income, and parenting style may each explain variability in youth externalizing problems; however, the interaction between income and parenting style was not significant. Findings have potential implications for better understanding the specific contexts in which externalizing problems may be most likely to occur within this at-risk and underserved group. PMID:26053349

  5. Marginal Effects of a Gross Income Increase for a Single Parent Family in Six European Countries

    DEFF Research Database (Denmark)

    Willumsen, Marie

    High marginal tax rates constitute an issue in several countries because they are supposed to create barriers for increased labour supply. It is, however, often the case that relatively low income families with children face substantially higher combined marginal rates than even the highest...... the contributions to the combined marginal rate, the marginal effective tax rate, METR, using the OECD term, from taxation, payment for childcare, tapering of housing benefits and sometimes child benefits, when the income varies from a low level to a high level for a single parent family. Six countries are included...... marginal tax rates. High combined marginal rates are generated by increasing payment for care for children in childcare institutions and tapering of housing benefits in addition to taxation, when income rises. These effects are often simultaneous and add to the marginal tax rate. This paper explores...

  6. Secondary Effects of an Alcohol Prevention Program Targeting Students and/or Parents.

    Science.gov (United States)

    Koning, Ina M; Vollebergh, Wilma A M

    2016-08-01

    The secondary effects of an alcohol prevention program (PAS) on onset of weekly smoking and monthly cannabis use are examined among >3000 Dutch early adolescents (M age=12.64) randomized over four conditions: 1) parent intervention (PI), 2) student intervention (SI), 3) combined intervention (CI) and 4) control condition (CC). Rules about alcohol, alcohol use, and adolescents' self-control were investigated as possible mediators. PI had a marginal aversive effect, slightly increasing the risk of beginning to smoke at T1, and increased the likelihood of beginning to use cannabis use at T1 and T2. SI delayed the onset of monthly cannabis use at T3. CI increased the risk to use cannabis at T3. No mediational processes were found. In conclusion, though this study show mixed results, negative side effects of the PI were found, particularly at earlier ages. Moreover, these results indicate the need for multi-target interventions. Copyright © 2016 Elsevier Inc. All rights reserved.

  7. A single gene target of an ETS-family transcription factor determines neuronal CO2-chemosensitivity

    DEFF Research Database (Denmark)

    Brandt, Julia P; Aziz-Zaman, Sonya; Juozaityte, Vaida

    2012-01-01

    . We report here a mechanism that endows C. elegans neurons with the ability to detect CO(2). The ETS-5 transcription factor is necessary for the specification of CO(2)-sensing BAG neurons. Expression of a single ETS-5 target gene, gcy-9, which encodes a receptor-type guanylate cyclase, is sufficient...

  8. Assessment of parental tooth-brushing following instruction with single-headed and triple-headed toothbrushes.

    Science.gov (United States)

    Telishevesky, Yoel S; Levin, Liran; Ashkenazi, Malka

    2012-01-01

    The purpose of this study was to evaluate the effect of toothbrush design on the ability of parents to effectively brush their children's teeth. Parents of children (mean age=5.1±0.75 years old) from 4 kindergarten schools were randomly assigned to receive instruction in brushing their children's teeth using a manual single-headed toothbrush (2 schools) or a triple-headed toothbrush (2 schools). The parents' ability to brush their children's teeth was evaluated according to a novel toothbrush performing skill index (Ashkenazi Index), based on 2 criteria: (1) placement of the toothbrush on each tooth segment to be brushed ("reach"); and (2) completion of enough strokes on each segment ("stay"). One month after instruction, tooth-brushing ability was re-evaluated and plaque index of the children's teeth was assessed. One month after instruction, parents using the triple-headed toothbrush received significantly higher scores on the tooth-brushing performance index (~86%), than did those in the single-headed group (~61%; P=.001). The plaque index was significantly higher in the single-headed group (0.97±0.38) vs the triple-headed group (0.72±0.29; Pbrushing performance index correlated negatively with the plaque index (Pbrushing by parents than does a single-headed toothbrush.

  9. Characterization of a mitochondrially targeted single-stranded DNA-binding protein in Arabidopsis thaliana.

    Science.gov (United States)

    Edmondson, Andrew C; Song, Daqing; Alvarez, Luis A; Wall, Melisa K; Almond, David; McClellan, David A; Maxwell, Anthony; Nielsen, Brent L

    2005-04-01

    A gene encoding a predicted mitochondrially targeted single-stranded DNA binding protein (mtSSB) was identified in the Arabidopsis thaliana genome sequence. This gene (At4g11060) codes for a protein of 201 amino acids, including a 28-residue putative mitochondrial targeting transit peptide. Protein sequence alignment shows high similarity between the mtSSB protein and single-stranded DNA binding proteins (SSB) from bacteria, including residues conserved for SSB function. Phylogenetic analysis indicates a close relationship between this protein and other mitochondrially targeted SSB proteins. The predicted targeting sequence was fused with the GFP coding region, and the organellar localization of the expressed fusion protein was determined. Specific targeting to mitochondria was observed in in-vitro import experiments and by transient expression of a GFP fusion construct in Arabidopsis leaves after microprojectile bombardment. The mature mtSSB coding region was overexpressed in Escherichia coli and the protein was purified for biochemical characterization. The purified protein binds single-stranded, but not double-stranded, DNA. MtSSB stimulates the homologous strand-exchange activity of E. coli RecA. These results indicate that mtSSB is a functional homologue of the E. coli SSB, and that it may play a role in mitochondrial DNA recombination.

  10. Comparison of Single and Dual Target Visual Attention Tasks in Children with down Syndrome

    Directory of Open Access Journals (Sweden)

    Melanie J. Murphy

    2011-05-01

    Full Text Available Understanding the nature of attentional processing in children with Down Syndrome (DS is imperative for developing effective education practices. The aim of the current study was to investigate whether children with DS exhibit impairment in sustained, transient, single-, or dual-target continuous performance tasks. Target detection time and accuracy was compared in children with DS to Typically Developing (TD children of similar nonverbal mental age (as measured by the Raven's Coloured Progressive Matrices, on single and dual- target continuous performance tasks measuring sustained attention, a visual change detection task measuring transient attention, and feature and conjunctive visual search tasks measuring both sustained and transient attention. Results showed that children with DS performed similarly to TD children on sustained and transient attention tasks that only required the detection of a single unique target, but were impaired in overall accuracy on tasks that required dual-target detection. Findings suggest a possible impairment in attention and working memory in children with DS. Error analysis of task responses revealed differences in problem solving strategy between children with DS and TD children, despite similar overall performance. Findings have implications for the education of children with DS and understanding of the nature of intellectual disability per se.

  11. Performance of autofocusing schemes for single target and populated scenes behind unknown walls

    Science.gov (United States)

    Ahmad, Fauzia; Amin, Moeness G.

    2007-04-01

    The quality and reliability of through-the-wall radar imagery is governed, among other things, by the knowledge of the wall characteristics. Ambiguity in wall characteristics has a two-fold effect. It smears and blurs the image, and also shifts the imaged target positions. Higher order standardized moments have been shown to be suitable measures of the degree of smearing and blurriness of through-the-wall images. These moments can be used to tune the wall variables to achieve autofocusing. It is noted that the solution to the autofocusing problem is not unique, and there exist several assumed wall characteristics, in addition to the exact, that lead to similar focused images. In this paper, we analyze the dependency of the estimated autofocusing wall parameters on the imaged scene, specifically target density and location, in the presence of single uniform wall. We consider single and multiple target cases with different scene complexity and population. Supporting simulation results are also provided.

  12. A Single Unexpected Change in Target- but Not Distractor Motion Impairs Multiple Object Tracking

    Directory of Open Access Journals (Sweden)

    Hauke S. Meyerhoff

    2013-02-01

    Full Text Available Recent research addresses the question whether motion information of multiple objects contributes to maintaining a selection of objects across a period of motion. Here, we investigate whether target and/or distractor motion information is used during attentive tracking. We asked participants to track four objects and changed either the motion direction of targets, the motion direction of distractors, neither, or both during a brief flash in the middle of a tracking interval. We observed that a single direction change of targets is sufficient to impair tracking performance. In contrast, changing the motion direction of distractors had no effect on performance. This indicates that target- but not distractor motion information is evaluated during tracking.

  13. Targeting neurotransmitter receptors with nanoparticles in vivo allows single-molecule tracking in acute brain slices

    Science.gov (United States)

    Varela, Juan A.; Dupuis, Julien P.; Etchepare, Laetitia; Espana, Agnès; Cognet, Laurent; Groc, Laurent

    2016-03-01

    Single-molecule imaging has changed the way we understand many biological mechanisms, particularly in neurobiology, by shedding light on intricate molecular events down to the nanoscale. However, current single-molecule studies in neuroscience have been limited to cultured neurons or organotypic slices, leaving as an open question the existence of fast receptor diffusion in intact brain tissue. Here, for the first time, we targeted dopamine receptors in vivo with functionalized quantum dots and were able to perform single-molecule tracking in acute rat brain slices. We propose a novel delocalized and non-inflammatory way of delivering nanoparticles (NPs) in vivo to the brain, which allowed us to label and track genetically engineered surface dopamine receptors in neocortical neurons, revealing inherent behaviour and receptor activity regulations. We thus propose a NP-based platform for single-molecule studies in the living brain, opening new avenues of research in physiological and pathological animal models.

  14. Quantitative estimation of plum pox virus targets acquired and transmitted by a single Myzus persicae.

    Science.gov (United States)

    Moreno, Aranzazu; Fereres, Alberto; Cambra, Mariano

    2009-01-01

    The viral charge acquired and inoculated by single aphids in a non-circulative transmission is estimated using plum pox virus (PPV). A combination of electrical penetration graph and TaqMan real-time RT-PCR techniques was used to establish the average number of PPV RNA targets inoculated by an aphid in a single probe (26,750), approximately half of the acquired ones. This number of PPV targets is responsible for a systemic infection of 20% on the inoculated receptor plants. No significant differences were found between the number of PPV RNA targets acquired after one and after five intracellular punctures (pd), but the frequency of infected receptor plants was higher after 5 pd. The percentage of PPV-positive leaf discs after just 1 pd of inoculation probe (28%/4,603 targets) was lower than after 5 pd (45.8%/135 x 10(6) targets). The methodology employed could be easily extended to other virus-vector-host combinations to improve the accuracy of models used in virus epidemiology.

  15. Promoting Parent and Child Physical Activity Together: Elicitation of Potential Intervention Targets and Preferences

    Science.gov (United States)

    Rhodes, Ryan E.; Lim, Clarise

    2018-01-01

    Promoting physical activities that involve both parents and their children would be very useful to the improved health and well-being of families, yet coactivity interventions have been particularly unsuccessful in past research. The purpose of this study was to elicit the salient parental beliefs about coactivity framed through theory of planned…

  16. Outcomes of a pilot obesity prevention plus intervention targeting children and parenting practices

    Science.gov (United States)

    Prevention-Plus interventions for primary care offer a venue to intervene with both children and parents for child obesity treatment. Such interventions can promote effective parenting practices that encourage healthy eating, physical activity (PA), and lower TV use among children. Test for feasibil...

  17. Targeted mutations induced by a single acetylaminofluorene DNA adduct in mammalian cells and bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Moryia, M.; Takeshita, M.; Johnson, F.; Peden, K.; Will, S.; Grollman, A.P.

    1988-03-01

    Mutagenic specificity of 2-acetylaminofluorene (AAF) has been established in mammalian cells and several strains of bacteria by using a shuttle plasmid vector containing a single N-(deoxyguanosin-8-yl)acetylaminofluorene (C8-dG-AAF) adduct. The nucleotide sequence of the gene conferring tetracycline resistance was modified by conservative codon replacement so as to accommodate the sequence d(CCTTCGCTAC) flanked by two restriction sites, Bsm I and Xho I. The corresponding synthetic oligodeoxynucleotide underwent reaction with 2-(N-acetoxy-N-acetylamino)-fluorene (AAAF), forming a single dG-AAF adduct. This modified oligodeoxynucleotide was hybridized to its complementary strand and ligated between the Bsm I and Xho I sites of the vector. Plasmids containing the C8-dG-AAF adduct were used to transfect simian virus 40-transformed simian kidney (COS-1) cells and to transform several AB strains of Escherichia coli. Colonies containing mutant plasmides were detected by hybridization to /sup 32/P-labeled oligodeoxynucleotides. Presence of the single DNA adduct increased the mutation frequency by 8-fold in both COS cells and E. coli. Over 80% of mutations detected in both systems were targeted and represented G x C ..-->.. C x G or G x C ..-->.. T x A transversions or single nucleotide deletions. The authors conclude that modification of a deoxyguanosine residue with AAF preferentially induces mutations targeted at this site when a plasmid containing a single C8-dG-AAF adduct is introduced into mammalian cells or bacteria.

  18. Single-Isocenter Multiple-Target Stereotactic Radiosurgery: Risk of Compromised Coverage

    International Nuclear Information System (INIS)

    Roper, Justin; Chanyavanich, Vorakarn; Betzel, Gregory; Switchenko, Jeffrey; Dhabaan, Anees

    2015-01-01

    Purpose: To determine the dosimetric effects of rotational errors on target coverage using volumetric modulated arc therapy (VMAT) for multitarget stereotactic radiosurgery (SRS). Methods and Materials: This retrospective study included 50 SRS cases, each with 2 intracranial planning target volumes (PTVs). Both PTVs were planned for simultaneous treatment to 21 Gy using a single-isocenter, noncoplanar VMAT SRS technique. Rotational errors of 0.5°, 1.0°, and 2.0° were simulated about all axes. The dose to 95% of the PTV (D95) and the volume covered by 95% of the prescribed dose (V95) were evaluated using multivariate analysis to determine how PTV coverage was related to PTV volume, PTV separation, and rotational error. Results: At 0.5° rotational error, D95 values and V95 coverage rates were ≥95% in all cases. For rotational errors of 1.0°, 7% of targets had D95 and V95 values <95%. Coverage worsened substantially when the rotational error increased to 2.0°: D95 and V95 values were >95% for only 63% of the targets. Multivariate analysis showed that PTV volume and distance to isocenter were strong predictors of target coverage. Conclusions: The effects of rotational errors on target coverage were studied across a broad range of SRS cases. In general, the risk of compromised coverage increased with decreasing target volume, increasing rotational error and increasing distance between targets. Multivariate regression models from this study may be used to quantify the dosimetric effects of rotational errors on target coverage given patient-specific input parameters of PTV volume and distance to isocenter.

  19. Targeting Parents for Childhood Weight Management: Development of a Theory-Driven and User-Centered Healthy Eating App.

    Science.gov (United States)

    Curtis, Kristina Elizabeth; Lahiri, Sudakshina; Brown, Katherine Elizabeth

    2015-06-18

    The proliferation of health promotion apps along with mobile phones' array of features supporting health behavior change offers a new and innovative approach to childhood weight management. However, despite the critical role parents play in children's weight related behaviors, few industry-led apps aimed at childhood weight management target parents. Furthermore, industry-led apps have been shown to lack a basis in behavior change theory and evidence. Equally important remains the issue of how to maximize users' engagement with mobile health (mHealth) interventions where there is growing consensus that inputs from the commercial app industry and the target population should be an integral part of the development process. The aim of this study is to systematically design and develop a theory and evidence-driven, user-centered healthy eating app targeting parents for childhood weight management, and clearly document this for the research and app development community. The Behavior Change Wheel (BCW) framework, a theoretically-based approach for intervention development, along with a user-centered design (UCD) philosophy and collaboration with the commercial app industry, guided the development process. Current evidence, along with a series of 9 focus groups (total of 46 participants) comprised of family weight management case workers, parents with overweight and healthy weight children aged 5-11 years, and consultation with experts, provided data to inform the app development. Thematic analysis of focus groups helped to extract information related to relevant theoretical, user-centered, and technological components to underpin the design and development of the app. Inputs from parents and experts working in the area of childhood weight management helped to identify the main target behavior: to help parents provide appropriate food portion sizes for their children. To achieve this target behavior, the behavioral diagnosis revealed the need for eliciting change in

  20. Metacognitive training for patients with schizophrenia: preliminary evidence for a targeted, single-module programme.

    Science.gov (United States)

    Balzan, Ryan P; Delfabbro, Paul H; Galletly, Cherrie A; Woodward, Todd S

    2014-12-01

    Metacognitive training is an eight-module, group-based treatment programme for people with schizophrenia that targets the cognitive biases (i.e. problematic thinking styles) thought to contribute to the genesis and maintenance of delusions. The present article is an investigation into the efficacy of a shorter, more targeted, single-module metacognitive training programme, administered individually, which focuses specifically on improving cognitive biases that are thought to be driven by a 'hypersalience of evidence-hypothesis matches' mechanism (e.g. jumping to conclusions, belief inflexibility, reasoning heuristics, illusions of control). It was hypothesised that a more targeted metacognitive training module could still improve performance on these bias tasks and reduce delusional ideation, while improving insight and quality of life. A sample of 28 patients diagnosed with schizophrenia and mild delusions either participated in the hour-long, single-session, targeted metacognitive training programme (n = 14), or continued treatment as usual (n = 14). All patients were assessed using clinical measures gauging overall positive symptomology, delusional ideation, quality of life and insight, and completed two cognitive bias tasks designed to elucidate the representativeness and illusion of control biases. After a 2-week, post-treatment interval, targeted metacognitive training patients exhibited significant decreases in delusional severity and conviction, significantly improved clinical insight, and significant improvements on the cognitive bias tasks, relative to the treatment-as-usual controls. Performance improvements on the cognitive bias tasks significantly correlated with the observed reductions in overall positive symptomology. Patients also evaluated the training positively. Although interpretations of these results are limited due to the lack of an optimally designed, randomised controlled trial and a small sample size, the results are promising and warrant

  1. Single freeform surface design for prescribed input wavefront and target irradiance.

    Science.gov (United States)

    Bösel, Christoph; Gross, Herbert

    2017-09-01

    In beam shaping applications, the minimization of the number of necessary optical elements for the beam shaping process can benefit the compactness of the optical system and reduce its cost. The single freeform surface design for input wavefronts, which are neither planar nor spherical, is therefore of interest. In this work, the design of single freeform surfaces for a given zero-étendue source and complex target irradiances is investigated. Hence, not only collimated input beams or point sources are assumed. Instead, a predefined input ray direction vector field and irradiance distribution on a source plane, which has to be redistributed by a single freeform surface to give the predefined target irradiance, is considered. To solve this design problem, a partial differential equation (PDE) or PDE system, respectively, for the unknown surface and its corresponding ray mapping is derived from energy conservation and the ray-tracing equations. In contrast to former PDE formulations of the single freeform design problem, the derived PDE of Monge-Ampère type is formulated for general zero-étendue sources in Cartesian coordinates. The PDE system is discretized with finite differences, and the resulting nonlinear equation system is solved by a root-finding algorithm. The basis of the efficient solution of the PDE system builds the introduction of an initial iterate construction approach for a given input direction vector field, which uses optimal mass transport with a quadratic cost function. After a detailed description of the numerical algorithm, the efficiency of the design method is demonstrated by applying it to several design examples. This includes the redistribution of a collimated input beam beyond the paraxial approximation, the shaping of point source radiation, and the shaping of an astigmatic input wavefront into a complex target irradiance distribution.

  2. Targeted genetics in Drosophila cell lines: Inserting single transgenes in vitro.

    Science.gov (United States)

    Manivannan, Sathiya N; Simcox, Amanda

    2016-07-02

    A long-standing problem with analyzing transgene expression in tissue-culture cells is the variation caused by random integration of different copy numbers of transfected transgenes. In mammalian cells, single transgenes can be inserted by homologous recombination but this process is inefficient in Drosophila cells. To tackle this problem, our group, and the Cherbas group, used recombination-mediated cassette exchange (RMCE) to introduce single-copy transgenes into specific locations in the Drosophila genome. In both cases, ϕC31 was used to catalyze recombination between its target sequences attP in the genome, and attB flanking the donor sequence. We generated cell lines de novo with a single attP-flanked cassette for recombination, whereas, Cherbas et al. introduced a single attP-flanked cassette into existing cell lines. In both approaches, a 2-drug selection scheme was used to select for cells with a single copy of the donor sequence inserted by RMCE and against cells with random integration of multiple copies. Here we describe the general advantages of using RMCE to introduce genes into fly cells, the different attributes of the 2 methods, and how future work could make use of other recombinases and CRISPR/Cas9 genome editing to further enable genetic manipulation of Drosophila cells in vitro.

  3. Chalcogenide thin films deposited by rfMS technique using a single quaternary target

    Science.gov (United States)

    Prepelita, P.; Stavarache, I.; Negrila, C.; Garoi, F.; Craciun, V.

    2017-12-01

    Thin films of chalcogenide, Cu(In,Ga)Se2 have been obtained using a single quaternary target by radio frequency magnetron sputtering method, with thickness in the range 750 nm to 1200 nm. X-ray photoelectron spectroscopy investigations showed, that the composition of Cu(In,Ga)Se2 thin films was very similar to that of the used target CuIn0.75Ga0.25Se2. Identification of the chemical composition of Cu(In,Ga)Se2 thin films by XPS performed in high vacuum, emphasized that the samples exhibit surface features suitable to be integrated into the structure of solar cells. Atomic Force Microscopy and Scanning Electron Microscopy investigations showed that surface morphology was influenced by the increase in thickness of the Cu(In,Ga)Se2 layer. From X-Ray Diffraction investigations it was found that all films were polycrystalline, having a tetragonal lattice with a preferential orientation along the (112) direction. The optical reflectance as a function of wavelength was measured for the studied samples. The increase in thickness of the Cu(In,Ga)Se2 absorber determined a decrease of its optical bandgap value from 1.53 eV to 1.44 eV. The results presented in this paper showed an excellent alternative of obtaining Cu(In,Ga)Se2 compound thin films from a single target.

  4. A Closer Look at Children in Single-Parent Families. ERIC/CUE Digest Number 23.

    Science.gov (United States)

    Roddy, Patricia Palker

    Schools are more and more called upon to accomodate students' differences in background and experiences; this picture of diversity includes the growing number of one-parent families. However, educators need to be cautioned against expecting "trouble" from the child from a one-parent family. The diversity among research findings suggests…

  5. Religiosity of Muslim Adolescents from Single Parent Families Living in Government-Subsidised Settlement

    Directory of Open Access Journals (Sweden)

    Fariza, M. S.

    2015-12-01

    Full Text Available Adolescence is a phase of emotionl turmoil and stress. If not balanced with religious practice, it may cause adolescents to fall into moral and social problems such as breach of school discipline, delinquency, drug addiction and sexual permissiveness. Religiosity is one way for adolescents to deal with challenges in life thereby avoiding immoral activities. This is because religiosity may help to balance adolescent emotional, cognitive and social developments.The purpose of this study is to identify the behaviour and level of religiosity among 30 adolescents from single parents families who settle in government-subsidised area of residence, namely known as the Federal Land Development Authority (FELDA. The area is located in the state of Pahang, situated on the eastern part of Malaysia. These adolescents have been selected as research respondents. This research was conducted as a survey using questionnaire as the research instrument. It was constructed based on the guidelines of Islamic Practice Instrument (IPI (Fariza, 2012 and has already been verified in terms of its validity and reliability. The data analysis has adopted the method of descriptive statistics explained in table form. The research results have found that religiosity or religious behaviour that the adolescents most frequently abide by are staying away from gambling, having good thoughts and expectations of Allah, feeling awed and fear of Allah’s power (taqwa, staying away from fornication (zina and supplication (doa to Allah facing the direction of Qibla. The level of religiosity is medium for most adolescents. This factor explains why not many respondents are involved in moral and disciplinary problems at school. This research finds that generally, religiosity protects adolescents from being involved in immoral activities and other vices.

  6. Transverse-target single-spin azimuthal asymmetry in hard exclusive electroproduction of single pions at HERMES

    International Nuclear Information System (INIS)

    Hristova, I.

    2007-12-01

    We present the analysis of data taken in the years 2002-2004 with the 27.56 GeV positron beam of the HERA storage ring at DESY and the internal transversely polarised hydrogen fixed target of the HERMES experiment. Events with a scattered positron and a produced pion are selected. Exclusive production of single pions, e + p→e +' nπ + , is ensured by requiring the missing mass in the event to be equal to the mass of the neutron, which is not detected. The cross section for this process depends on the Bjorken scaling variable, the four-momentum transfer, and the transverse four-momentum transfer, whose average values for our sample are left angle x right angle =0.12, left angle Q 2 right angle =2.3 GeV 2 , left angle t' right angle =-0.18 GeV 2 , respectively, and two azimuthal angles: the angle φ between the scattering and production planes (their common line contains the virtual photon), and the angle φ S between the scattering plane and the target polarisation vector. The hard scattering is selected by requiring Q 2 >1 GeV 2 . The asymmetry, also called transverse-target single-spin azimuthal asymmetry, is defined as the ratio of the difference to the sum of the cross sections for positive and negative target polarisation. It is characterised by six azimuthal sine modulations, whose amplitudes can vary from -1 to 1. We measure the asymmetry from a sample of 2093 events with a signal-to-background ratio of 1: 1. At average kinematics, the values of the amplitudes are found to be small or consistent with zero, except for the amplitude A sinφ S UT,meas =0.38±0.06(stat) +0.12 -0.06 (syst). The amplitude of main interest for comparison with theory, A sin(φ-φ S ) UT,meas =0.09±0.05(stat) +0.10 -0.03 (syst), after correction for the background contribution becomes A sin(φ-φ S ) UT,bg.cor =0.22 ±0.13(stat) +0.10 -0.04 (syst). As a function of t', the measured values of this amplitude increase as √(-t') and at larger vertical stroke t' vertical stroke the

  7. Transverse-target single-spin azimuthal asymmetry in hard exclusive electroproduction of single pions at HERMES

    Energy Technology Data Exchange (ETDEWEB)

    Hristova, I.

    2007-12-15

    We present the analysis of data taken in the years 2002-2004 with the 27.56 GeV positron beam of the HERA storage ring at DESY and the internal transversely polarised hydrogen fixed target of the HERMES experiment. Events with a scattered positron and a produced pion are selected. Exclusive production of single pions, e{sup +}p{yields}e{sup +'}n{pi}{sup +}, is ensured by requiring the missing mass in the event to be equal to the mass of the neutron, which is not detected. The cross section for this process depends on the Bjorken scaling variable, the four-momentum transfer, and the transverse four-momentum transfer, whose average values for our sample are left angle x right angle =0.12, left angle Q{sup 2} right angle =2.3 GeV{sup 2}, left angle t' right angle =-0.18 GeV{sup 2}, respectively, and two azimuthal angles: the angle {phi} between the scattering and production planes (their common line contains the virtual photon), and the angle {phi}{sub S} between the scattering plane and the target polarisation vector. The hard scattering is selected by requiring Q{sup 2}>1 GeV{sup 2}. The asymmetry, also called transverse-target single-spin azimuthal asymmetry, is defined as the ratio of the difference to the sum of the cross sections for positive and negative target polarisation. It is characterised by six azimuthal sine modulations, whose amplitudes can vary from -1 to 1. We measure the asymmetry from a sample of 2093 events with a signal-to-background ratio of 1: 1. At average kinematics, the values of the amplitudes are found to be small or consistent with zero, except for the amplitude A{sup sin{phi}{sub SUT,meas}}=0.38{+-}0.06(stat){sup +0.12}{sub -0.06}(syst). The amplitude of main interest for comparison with theory, A{sup sin({phi}-{phi}{sub S})}{sub UT,meas}=0.09{+-}0.05(stat){sup +0.10}{sub -0.03}(syst), after correction for the background contribution becomes A{sup sin({phi}-{phi}{sub S})}{sub UT,bg.cor}=0.22 {+-}0.13(stat){sup +0.10}{sub -0

  8. Induction of associative olfactory memory by targeted activation of single olfactory neurons in Drosophila larvae.

    Science.gov (United States)

    Honda, Takato; Lee, Chi-Yu; Yoshida-Kasikawa, Maki; Honjo, Ken; Furukubo-Tokunaga, Katsuo

    2014-04-25

    It has been postulated that associative memory is formed by at least two sets of external stimuli, CS and US, that are transmitted to the memory centers by distinctive conversing pathways. However, whether associative memory can be induced by the activation of only the olfactory CS and a biogenic amine-mediated US pathways remains to be elucidated. In this study, we substituted the reward signals with dTrpA1-mediated thermogenetic activation of octopaminergic neurons and the odor signals by ChR2-mediated optical activation of a specific class of olfactory neurons. We show that targeted activation of the olfactory receptor and the octopaminergic neurons is indeed sufficient for the formation of associative olfactory memory in the larval brain. We also show that targeted stimulation of only a single type of olfactory receptor neurons is sufficient to induce olfactory memory that is indistinguishable from natural memory induced by the activation of multiple olfactory receptor neurons.

  9. The "curved lead pathway" method to enable a single lead to reach any two intracranial targets.

    Science.gov (United States)

    Ding, Chen-Yu; Yu, Liang-Hong; Lin, Yuan-Xiang; Chen, Fan; Lin, Zhang-Ya; Kang, De-Zhi

    2017-01-11

    Deep brain stimulation is an effective way to treat movement disorders, and a powerful research tool for exploring brain functions. This report proposes a "curved lead pathway" method for lead implantation, such that a single lead can reach in sequence to any two intracranial targets. A new type of stereotaxic system for implanting a curved lead to the brain of human/primates was designed, the auxiliary device needed for this method to be used in rat/mouse was fabricated and verified in rat, and the Excel algorithm used for automatically calculating the necessary parameters was implemented. This "curved lead pathway" method of lead implantation may complement the current method, make lead implantation for multiple targets more convenient, and expand the experimental techniques of brain function research.

  10. Single-Labeled Oligonucleotides Showing Fluorescence Changes upon Hybridization with Target Nucleic Acids

    Directory of Open Access Journals (Sweden)

    Gil Tae Hwang

    2018-01-01

    Full Text Available Sequence-specific detection of nucleic acids has been intensively studied in the field of molecular diagnostics. In particular, the detection and analysis of single-nucleotide polymorphisms (SNPs is crucial for the identification of disease-causing genes and diagnosis of diseases. Sequence-specific hybridization probes, such as molecular beacons bearing the fluorophore and quencher at both ends of the stem, have been developed to enable DNA mutation detection. Interestingly, DNA mutations can be detected using fluorescently labeled oligonucleotide probes with only one fluorophore. This review summarizes recent research on single-labeled oligonucleotide probes that exhibit fluorescence changes after encountering target nucleic acids, such as guanine-quenching probes, cyanine-containing probes, probes containing a fluorophore-labeled base, and microenvironment-sensitive probes.

  11. Single fraction radiosurgery using Rapid Arc for treatment of intracranial targets

    International Nuclear Information System (INIS)

    Wolff, Hendrik A; Wagner, Daniela M; Christiansen, Hans; Hess, Clemens F; Vorwerk, Hilke

    2010-01-01

    Stereotactic-Radio-Surgery (SRS) using Conformal-Arc-Therapy (CAT) is a well established irradiation technique for treatment of intracranial targets. Although small safety margins are required because of very high accuracy of patient positioning and exact online localisation, there are still disadvantages like long treatment time, high number of monitor units (MU) and covering of noncircular targets. This planning study analysed whether Rapid Arc (RA) with stereotactic localisation for single-fraction SRS can solve these problems. Ten consecutive patients were treated with Linac-based SRS. Eight patients had one or more brain metastases. The other patients presented a symptomatic vestibularis schwannoma and an atypic meningeoma. For all patients, two plans (CAT/RA) were calculated and analysed. Conformity was higher for RA with additional larger low-dose areas. Furthermore, RA reduced the number of MU and the treatment time for all patients. Dose to organs at risk were equal or slightly higher using RA in comparison to CAT. RA provides a new alternative for single-fraction SRS irradiation combining advantages of short treatment time with lower number of MU and better conformity in addition to accuracy of stereotactic localisation in selected cases with uncomplicated clinical realization

  12. A Synthetic Biology Project - Developing a single-molecule device for screening drug-target interactions.

    Science.gov (United States)

    Firman, Keith; Evans, Luke; Youell, James

    2012-07-16

    This review describes a European-funded project in the area of Synthetic Biology. The project seeks to demonstrate the application of engineering techniques and methodologies to the design and construction of a biosensor for detecting drug-target interactions at the single-molecule level. Production of the proteins required for the system followed the principle of previously described "bioparts" concepts (a system where a database of biological parts - promoters, genes, terminators, linking tags and cleavage sequences - is used to construct novel gene assemblies) and cassette-type assembly of gene expression systems (the concept of linking different "bioparts" to produce functional "cassettes"), but problems were quickly identified with these approaches. DNA substrates for the device were also constructed using a cassette-system. Finally, micro-engineering was used to build a magnetoresistive Magnetic Tweezer device for detection of single molecule DNA modifying enzymes (motors), while the possibility of constructing a Hall Effect version of this device was explored. The device is currently being used to study helicases from Plasmodium as potential targets for anti-malarial drugs, but we also suggest other potential uses for the device. Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

  13. Intraoperative Boost Radiotherapy during Targeted Oncoplastic Breast Surgery: Overview and Single Center Experiences

    Directory of Open Access Journals (Sweden)

    Wolfram Malter

    2014-01-01

    Full Text Available Breast-conserving surgery followed by whole-breast irradiation is the standard local therapy for early breast cancer. The international discussion of reduced importance of wider tumor-free resection margins than “tumor not touching ink” leads to the development of five principles in targeted oncoplastic breast surgery. IORT improves local recurrence risk and diminishes toxicity since there is less irradiation of healthy tissue. Intraoperative radiotherapy (IORT can be delivered in two settings: an IORT boost followed by a conventional regimen of external beam radiotherapy or a single IORT dose. The data from TARGIT-A and ELIOT reinforce the conviction that intraoperative radiotherapy during breast-conserving surgery is a reliable alternative to conventional postoperative fractionated irradiation, but only in a carefully selected population at low risk of local recurrence. We describe our experiences with IORT boost (50 kV energy X-rays; 20 Gy in combination with targeted oncoplastic breast surgery in a routine clinical setting. Our experiences demonstrate the applicability and reliability of combining IORT boost with targeted oncoplastic breast surgery in breast-conserving therapy of early breast cancer.

  14. Process evaluation of the parental component in the Boost study - a school-randomized trial targeting fruit and vegetable intake among Danish adolescents

    DEFF Research Database (Denmark)

    Jørgensen, Sanne Ellegaard; Jørgensen, Thea Suldrup; Aarestrup, Anne Kristine

    Purpose: School-based dietary interventions often include a parental component, but the degree of implementation is seldom reported. This study evaluated the implementation of six parental newsletters in the Boost study, a multicomponent school-randomized controlled trial targeting fruit...... and vegetable intake among year 7th graders (~13-year-olds) in school year 2010/11. Methods: Post-intervention questionnaire data from parents and teachers at 20 intervention schools were analysed descriptively. Process measures: Dose delivered: number of newsletters uploaded by teachers to the school’s website....... Dose received: if parents had seen and read the newsletters. Appreciation: perceived usefulness of newsletters. Reach: dose received stratified by gender and parental occupational social class (OSC). Results: Questionnaires were completed by parents of 58.7% of the students (N=658) and by teachers...

  15. Transverse target single-spin asymmetry in inclusive electroproduction of charged pions and kaons

    Energy Technology Data Exchange (ETDEWEB)

    Airapetian, A. [Giessen Univ. (Germany). 2. Physikalisches Inst.; Michigan Univ., Ann Arbor, MI (United States). Randall Laboratory of Physics; Akopov, N. [Yerevan Physics Institute (Argentina); Akopov, Z. [DESY Hamburg (Germany)] [and others; Collaboration: HERMES Collaboration

    2013-10-15

    Single-spin asymmetries were investigated in inclusive electroproduction of charged pions and kaons from transversely polarized protons at the HERMES experiment. The asymmetries were studied as a function of the azimuthal angle {psi} about the beam direction between the target-spin direction and the hadron production plane, the transverse hadron momentum P{sub T} relative to the direction of the incident beam, and the Feynman variable x{sub F}. The sin {psi} amplitudes are positive for {pi}{sup +} and K{sup +}, slightly negative for {pi}{sup -} consistent with zero for K{sup -}, with particular P{sub T} but weak x{sub F} dependences. Especially large asymmetries are observed for two small subsamples of events, where also the scattered electron was recorded by the spectrometer.

  16. Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations

    DEFF Research Database (Denmark)

    da Silva, Ines Isabel Cardoso Rodrigues; Jers, Carsten; Otten, Harm

    2014-01-01

    Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2.-) catalyze cleavage of α-1,4 bonds between rhamnose and galacturonic acid in the backbone of pectins by β-elimination. In the present study, targeted improvement of the thermostability of a PL family 11 RGI lyase from Bacillus licheniformis (DSM...... the wild-type RGI lyase in Bacillus subtilis as opposed to in Pichia pastoris; this effect is suggested to be a negative result of glycosylation of the P. pastoris expressed enzyme. A ~ twofold improvement in thermal stability at 60 °C, accompanied by less significant increases in Tm of the enzyme mutants......, were obtained due to additive stabilizing effects of single amino acid mutations (E434L, G55V, and G326E) compared to the wild type. The crystal structure of the B. licheniformis wild-type RGI lyase was also determined; the structural analysis corroborated that especially mutation of charged amino...

  17. A single gene target of an ETS-family transcription factor determines neuronal CO2-chemosensitivity.

    Directory of Open Access Journals (Sweden)

    Julia P Brandt

    Full Text Available Many animals possess neurons specialized for the detection of carbon dioxide (CO(2, which acts as a cue to elicit behavioral responses and is also an internally generated product of respiration that regulates animal physiology. In many organisms how such neurons detect CO(2 is poorly understood. We report here a mechanism that endows C. elegans neurons with the ability to detect CO(2. The ETS-5 transcription factor is necessary for the specification of CO(2-sensing BAG neurons. Expression of a single ETS-5 target gene, gcy-9, which encodes a receptor-type guanylate cyclase, is sufficient to bypass a requirement for ets-5 in CO(2-detection and transforms neurons into CO(2-sensing neurons. Because ETS-5 and GCY-9 are members of gene families that are conserved between nematodes and vertebrates, a similar mechanism might act in the specification of CO(2-sensing neurons in other phyla.

  18. Transverse target single-spin asymmetry in inclusive electroproduction of charged pions and kaons

    Energy Technology Data Exchange (ETDEWEB)

    Airapetian, A. [II. Physikalisches Institut, Justus-Liebig-Universität Gießen, 35392 Gießen (Germany); Randall Laboratory of Physics, University of Michigan, Ann Arbor, MI 48109-1040 (United States); Akopov, N. [Yerevan Physics Institute, 375036 Yerevan (Armenia); Akopov, Z. [DESY, 22603 Hamburg (Germany); Aschenauer, E.C. [DESY, 15738 Zeuthen (Germany); Augustyniak, W. [National Centre for Nuclear Research, 00-689 Warsaw (Poland); Avakian, R.; Avetissian, A. [Yerevan Physics Institute, 375036 Yerevan (Armenia); Avetisyan, E. [DESY, 22603 Hamburg (Germany); Belostotski, S. [K.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, 188300 Leningrad Region (Russian Federation); Bianchi, N. [Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali di Frascati, 00044 Frascati (Italy); Blok, H.P. [National Institute for Subatomic Physics (Nikhef), 1009 DB Amsterdam (Netherlands); Department of Physics and Astronomy, VU University, 1081 HV Amsterdam (Netherlands); Borissov, A. [DESY, 22603 Hamburg (Germany); Bowles, J. [SUPA, School of Physics and Astronomy, University of Glasgow, Glasgow G12 8QQ (United Kingdom); Bryzgalov, V. [Institute for High Energy Physics, Protvino, 142281 Moscow Region (Russian Federation); Burns, J. [SUPA, School of Physics and Astronomy, University of Glasgow, Glasgow G12 8QQ (United Kingdom); Capiluppi, M. [Istituto Nazionale di Fisica Nucleare, Sezione di Ferrara and Dipartimento di Fisica e Scienze della Terra, Università di Ferrara, 44122 Ferrara (Italy); Capitani, G.P. [Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali di Frascati, 00044 Frascati (Italy); Cisbani, E. [Istituto Nazionale di Fisica Nucleare, Sezione di Roma, Gruppo Collegato Sanità and Istituto Superiore di Sanità, 00161 Roma (Italy); and others

    2014-01-20

    Single-spin asymmetries were investigated in inclusive electroproduction of charged pions and kaons from transversely polarized protons at the HERMES experiment. The asymmetries were studied as a function of the azimuthal angle ψ about the beam direction between the target-spin direction and the hadron production plane, the transverse hadron momentum P{sub T} relative to the direction of the incident beam, and the Feynman variable x{sub F}. The sin ψ amplitudes are positive for π{sup +} and K{sup +}, slightly negative for π{sup −} and consistent with zero for K{sup −}, with particular P{sub T} but weak x{sub F} dependences. Especially large asymmetries are observed for two small subsamples of events, where also the scattered electron was recorded by the spectrometer.

  19. EDUCATIONAL TACTICS OF MOTHERS AND THEIR RELATION TO THE PSYCHOLOGICAL CHARACTERISTICS OF ADOLESCENTS IN TWO-PARENT AND SINGLE-PARENT FAMILIES

    Directory of Open Access Journals (Sweden)

    Константин Борисович Зуев

    2013-04-01

    Full Text Available The paper presents the results of a study of the psychological characteristics of boys and girls from complete and incomplete families. In addition to the type of family, the authors consider the educational tactics of mothers. The combined effects of the type of family and educational tactics on psychological characteristics of the adolescent were investigated. Adolescence was chosen as a period, when on the one hand, a relatively stable personality structure is developed, and on the other hand, the importance of the immediate social environment is extremely high. For our study we selected the psychological characteristics, to the utmost revealing the reaction of children to their parents' divorce: the level of subjective control (degree of responsibility for their own lives, and the sovereignty of the psychological space (clearnesse of psychological boundaries. It is shown that the largest influence on the psychological characteristics of adolescents, regardless of the type of family, is rendered by hostility in maternal education. The dependence of psychological characteristics of maternal directiveness occurs only at high values of such educational tactics that highlights the consistency of the obtained results with the classical studies of single-parent families.DOI: http://dx.doi.org/10.12731/2218-7405-2013-3-6

  20. Field Geometric Calibration Method for Line Structured Light Sensor Using Single Circular Target

    Directory of Open Access Journals (Sweden)

    Tianfei Chen

    2017-01-01

    Full Text Available To achieve fast calibration of line structured light sensor, a geometric calibration approach based on single circular calibration target is proposed. The proposed method uses the circular points to establish linear equations, and according to the angle constraint, the camera intrinsic parameters can be calculated through optimization. Then, the light plane calibration is accomplished in two steps. Firstly, when the vanishing lines of target plane at various postures are obtained, the intersections between vanishing lines and laser stripe can be computed, and the normal vector of light plane can be calibrated via line fitting method using intersection points. After that, the distance from the origin of camera coordinate system to the light plane can be derived based on the model of perspective-three-point. The actual experimental result shows that this calibration method has high accuracy, its average measuring accuracy is 0.0451 mm, and relative error is 0.2314%. In addition, the entire calibration process has no complex operations. It is simple, convenient, and suitable for calibration on sites.

  1. Plasmonic welded single walled carbon nanotubes on monolayer graphene for sensing target protein

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jangheon; Kim, Soohyun [Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 373-1 Guseong, Yuseong, Daejeon 305-806 (Korea, Republic of); Kim, Gi Gyu; Jung, Wonsuk, E-mail: wonsuk81@wku.ac.kr [Department of Mechanical and Automotive Engineering, Wonkwang University, Iksan, Jeonbuk 570-749 (Korea, Republic of)

    2016-05-16

    We developed plasmonic welded single walled carbon nanotubes (SWCNTs) on monolayer graphene as a biosensor to detect target antigen molecules, fc fusion protein without any treatment to generate binder groups for linker and antibody. This plasmonic welding induces atomic networks between SWCNTs as junctions containing carboxylic groups and improves the electrical sensitivity of a SWCNTs and the graphene membrane to detect target protein. We investigated generation of the atomic networks between SWCNTs by field-emission scanning electron microscopy and atomic force microscopy after plasmonic welding process. We compared the intensity ratios of D to G peaks from the Raman spectra and electrical sheet resistance of welded SWCNTs with the results of normal SWCNTs, which decreased from 0.115 to 0.086 and from 10.5 to 4.12, respectively. Additionally, we measured the drain current via source/drain voltage after binding of the antigen to the antibody molecules. This electrical sensitivity of the welded SWCNTs was 1.55 times larger than normal SWCNTs.

  2. Single-Domain Antibodies and the Promise of Modular Targeting in Cancer Imaging and Treatment

    Directory of Open Access Journals (Sweden)

    María Elena Iezzi

    2018-02-01

    Full Text Available Monoclonal antibodies and their fragments have significantly changed the outcome of cancer in the clinic, effectively inhibiting tumor cell proliferation, triggering antibody-dependent immune effector cell activation and complement mediated cell death. Along with a continued expansion in number, diversity, and complexity of validated tumor targets there is an increasing focus on engineering recombinant antibody fragments for lead development. Single-domain antibodies (sdAbs, in particular those engineered from the variable heavy-chain fragment (VHH gene found in Camelidae heavy-chain antibodies (or IgG2 and IgG3, are the smallest fragments that retain the full antigen-binding capacity of the antibody with advantageous properties as drugs. For similar reasons, growing attention is being paid to the yet smaller variable heavy chain new antigen receptor (VNAR fragments found in Squalidae. sdAbs have been selected, mostly from immune VHH libraries, to inhibit or modulate enzyme activity, bind soluble factors, internalize cell membrane receptors, or block cytoplasmic targets. This succinct review is a compilation of recent data documenting the application of engineered, recombinant sdAb in the clinic as epitope recognition “modules” to build monomeric, dimeric and multimeric ligands that target, tag and stall solid tumor growth in vivo. Size, affinity, specificity, and the development profile of sdAbs drugs are seemingly consistent with desirable clinical efficacy and safety requirements. But the hepatotoxicity of the tetrameric anti-DR5-VHH drug in patients with pre-existing anti-drug antibodies halted the phase I clinical trial and called for a thorough pre-screening of the immune and poly-specific reactivities of the sdAb leads.

  3. Listening to children: gaining a perspective of the experiences of poverty and social exclusion from children and young people of single-parent families.

    Science.gov (United States)

    Walker, Janet; Crawford, Karin; Taylor, Francesca

    2008-07-01

    This paper reports on the experiences and views of children and young people of single-parent families, as findings from a European Union-funded research project undertaken in England, Greece and Cyprus. The objectives of the research project were to investigate how children and young people of single-parent families understand and experience their worlds as members of these families: whether and to what extent they experience poverty and social exclusion and how they cope with the challenges that this confronts them with. Methodology was replicated in each of the countries; however, this paper draws on the English experiences. Semistructured interviews (40) and focus groups (four) were undertaken with children of single parents. In addition, focus groups were undertaken with children of two-parent families (four), focus groups with single parents, focus groups with two-parent families (four) and individual interviews with key professionals. Detailed discussion guides were followed, with open-ended questions to allow participants to express their feelings and ideas in their own words. The research sample included children from single-parent and two-parent families, aged 6 years to 16 years, balanced in terms of age, gender and geographical location. Findings demonstrate the children's and young people's understanding of the impact of poverty and social exclusion on their family life and their everyday experiences. The positive benefits of being in a single-parent family are highlighted, with 'time poverty' raised as a significant issue. Children and young people are aware of their poverty and how it influences exclusion from friendships, play, leisure and community activities. Policy needs to take account of the systematic reality of children's experiences; alliances with adults that support meaningful involvement and participation by children and young people will make a significant contribution to this.

  4. Secondary emission from a CuBe target due to bombardment with parent and fragment ions of ammonia and phosphine

    International Nuclear Information System (INIS)

    Maerk, T.D.

    1977-01-01

    The secondary electron emission of the first dynode of a CuBe alloy sixteen dynode electron multiplier has been studied in the course of electron impact ionization studies of ammonia and phosphine. Relative secondary electron emission coefficients have been obtained for the singly and doubly charged parent and fragment ions of ammonia, ammonia-d 3 , phosphine and phosphine-d 3 for kinetic energies of 5,25 and 10,5 keV. It has been found, that in general deuterated ions have smaller γ coefficients, that ammonia ions have larger γ coefficients than corresponding phosphine ions, and that the γ coefficients increase with the complexity of the ion under study. (Auth.)

  5. Cognitive Vulnerabilities to Depression for Adolescents in Single-Mother and Two-Parent Families

    Science.gov (United States)

    Daryanani, Issar; Hamilton, Jessica L.; McArthur, Brae Anne; Steinberg, Laurence; Abramson, Lyn. Y.; Alloy, Lauren B.

    2017-01-01

    Although research consistently suggests that adolescents in single-mother families are at increased risk for depression, the mechanisms that explain this relationship are unclear. In a community sample of adolescents (N = 368; ages 12–16; 50% female; 50% White) and their mothers (42% single), adolescents completed measures of depressive symptoms, rumination, and depressogenic inferential style at baseline and two yearly follow-ups. Mothers reported on stressful events that occurred in the child’s life from birth until baseline. Adolescents raised by single mothers, relative to partnered mothers, experienced more childhood stressors and higher rumination levels at one-year follow-up. Additionally, higher rumination mediated the relationship between single motherhood and greater youth depressive symptoms at the two-year follow-up. Clinical implications and developmental considerations are discussed. PMID:27858293

  6. Happy eating: the single target implicit association test predicts overeating after positive emotions.

    Science.gov (United States)

    Bongers, Peggy; Jansen, Anita; Houben, Katrijn; Roefs, Anne

    2013-08-01

    For many years, questionnaires have been considered the standard when examining emotional eating behavior. However, recently, some controversy has arisen about these questionnaires, and their usefulness in identifying emotional eaters has been questioned. The current study aimed to investigate the Single Target Implicit Association Test (ST-IAT) as a measure of emotional eating. Two ST-IATs (assessing food-positive and food-negative associations respectively) and the Dutch Eating Behaviour Questionnaire (DEBQ) were compared in undergraduate students. A positive, negative or neutral mood was induced by means of a film clip, and milkshake consumption was measured during and after the mood induction. It was hypothesized that participants with strong emotion-food associations on the ST-IATs (i.e., IAT-emotional eaters) would consume more food in the emotion induction condition corresponding to that emotion, as compared to those with weak emotion-food associations as well as to those in the neutral condition. Participants who scored high on both the positive and negative ST-IATs ate more during a positive mood induction than during a negative mood induction. This effect did not extend to milkshake consumption after the mood induction procedure. In addition, IAT-positive emotional eaters consumed more food than IAT-non-emotional eaters. No effects of the DEBQ on milkshake consumption were found. It is concluded that the ST-IAT has potential as a measure of emotional eating. © 2013 Elsevier Ltd. All rights reserved.

  7. Utilizing Retinotopic Mapping for a Multi-Target SSVEP BCI With a Single Flicker Frequency.

    Science.gov (United States)

    Maye, Alexander; Zhang, Dan; Engel, Andreas K

    2017-07-01

    In brain-computer interfaces (BCIs) that use the steady-state visual evoked response (SSVEP), the user selects a control command by directing attention overtly or covertly to one out of several flicker stimuli. The different control channels are encoded in the frequency, phase, or time domain of the flicker signals. Here, we present a new type of SSVEP BCI, which uses only a single flicker stimulus and yet affords controlling multiple channels. The approach rests on the observation that the relative position between the stimulus and the foci of overt attention result in distinct topographies of the SSVEP response on the scalp. By classifying these topographies, the computer can determine at which position the user is gazing. Offline data analysis in a study on 12 healthy volunteers revealed that 9 targets can be recognized with about 95±3% accuracy, corresponding to an information transfer rate (ITR) of 40.8 ± 3.3 b/min on average. We explored how the classification accuracy is affected by the number of control channels, the trial length, and the number of EEG channels. Our findings suggest that the EEG data from five channels over parieto-occipital brain areas are sufficient for reliably classifying the topographies and that there is a large potential to improve the ITR by optimizing the trial length. The robust performance and the simple stimulation setup suggest that this approach is a prime candidate for applications on desktop and tablet computers.

  8. Targeting single-walled carbon nanotubes for the treatment of breast cancer using photothermal therapy

    International Nuclear Information System (INIS)

    Neves, Luís F F; Krais, John J; Van Rite, Brent D; Harrison, Roger G; Ramesh, Rajagopal; Resasco, Daniel E

    2013-01-01

    This paper focuses on the targeting of single-walled carbon nanotubes (SWNTs) for the treatment of breast cancer with minimal side effects using photothermal therapy. The human protein annexin V (AV) binds specifically to anionic phospholipids expressed externally on the surface of tumour cells and endothelial cells that line the tumour vasculature. A 2 h incubation of the SWNT-AV conjugate with proliferating endothelial cells followed by washing and near-infrared (NIR) irradiation at a wavelength of 980 nm was enough to induce significant cell death; there was no significant cell death with irradiation or the conjugate alone. Administration of the same conjugate i.v. in BALB/c female mice with implanted 4T1 murine mammary at a dose of 0.8 mg SWNT kg −1 and followed one day later by NIR irradiation of the tumour at a wavelength of 980 nm led to complete disappearance of implanted 4T1 mouse mammary tumours for the majority of the animals by 11 days since the irradiation. The combination of the photothermal therapy with the immunoadjuvant cyclophosphamide resulted in increased survival. The in vivo results suggest the SWNT-AV/NIR treatment is a promising approach to treat breast cancer. (paper)

  9. Application of real-time single camera SLAM technology for image-guided targeting in neurosurgery

    Science.gov (United States)

    Chang, Yau-Zen; Hou, Jung-Fu; Tsao, Yi Hsiang; Lee, Shih-Tseng

    2012-10-01

    In this paper, we propose an application of augmented reality technology for targeting tumors or anatomical structures inside the skull. The application is a combination of the technologies of MonoSLAM (Single Camera Simultaneous Localization and Mapping) and computer graphics. A stereo vision system is developed to construct geometric data of human face for registration with CT images. Reliability and accuracy of the application is enhanced by the use of fiduciary markers fixed to the skull. The MonoSLAM keeps track of the current location of the camera with respect to an augmented reality (AR) marker using the extended Kalman filter. The fiduciary markers provide reference when the AR marker is invisible to the camera. Relationship between the markers on the face and the augmented reality marker is obtained by a registration procedure by the stereo vision system and is updated on-line. A commercially available Android based tablet PC equipped with a 320×240 front-facing camera was used for implementation. The system is able to provide a live view of the patient overlaid by the solid models of tumors or anatomical structures, as well as the missing part of the tool inside the skull.

  10. Linking Single Domain Antibodies that Recognize Different Epitopes on the Same Target

    Directory of Open Access Journals (Sweden)

    Ellen R. Goldman

    2012-02-01

    Full Text Available Single domain antibodies (sdAb are the recombinantly expressed variable regions from the heavy-chain-only antibodies found in camelids and sharks. SdAb are able to bind antigens with high affinity, and most are capable of refolding after heat or chemical denaturation to bind antigen again. Starting with our previously isolated ricin binding sdAb determined to bind to four non-overlapping epitopes, we constructed a series of sdAb pairs, which were genetically linked through peptides of different length. We designed the series so that the sdAb are linked in both orientations with respect to the joining peptide. We confirmed that each of the sdAb in the constructs was able to bind to the ricin target, and have evidence that they are both binding ricin simultaneously. Through this work we determined that the order of genetically linked sdAb seems more important than the linker length. The genetically linked sdAb allowed for improved ricin detection with better limits of detection than the best anti-ricin monoclonal we evaluated, however they were not able to refold as well as unlinked component sdAb.

  11. Single base mismatches in the mRNA target site allow specific seed region-mediated off-target binding of siRNA targeting human coagulation factor 7.

    Science.gov (United States)

    Ravon, Morgane; Berrera, Marco; Ebeling, Martin; Certa, Ulrich

    2012-01-01

    We have analyzed the off-target activity of two siRNAs (F7-1, F7-2) that knock-down human blood coagulation factor 7 mRNA. F7-1 modulates a significant number of non-target transcripts while F7-2 shows high selectivity for the target transcript under various experimental conditions. The 3'-UTRs of all F7-1 off-target genes show statistically significant enrichment of the reverse complement of the F7-1 siRNA seed region located in the guide strand. Seed region enrichment was confirmed in off-target transcripts modulated by siRNA targeting the glucocorticoid receptor. To investigate how these sites contribute to off-target recognition of F7-1, we employed CXCL5 transcript as model system because it contains five F7-1 seed sequence motifs with single base mismatches. We show by transient transfection of reporter gene constructs into HEK293 cells that three out of five sites located in the 3'-UTR region are required for F7-1 off-target activity. For further mechanistic dissection, the sequences of these sites were synthesized and inserted either individually or joined in dimeric or trimeric constructs. Only the fusion constructs were silenced by F7-1 while the individual sites had no off-target activity. Based on F7-1 as a model, a single mismatch between the siRNA seed region and mRNA target sites is tolerated for target recognition and the CXCL5 data suggest a requirement for binding to multiple target sites in off-target transcripts.

  12. A Gas Target Internal to the LHC for the Study of pp Single-Spin Asymmetries and Heavy Ion Collisions

    Directory of Open Access Journals (Sweden)

    Colin Barschel

    2015-01-01

    Full Text Available We discuss the application of an open storage cell as gas target for a proposed LHC fixed-target experiment AFTER@LHC. The target provides a high areal density at minimum gas input, which may be polarized 1H, 2H, or 3He gas or heavy inert gases in a wide mass range. For the study of single-spin asymmetries in pp interaction, luminosities of nearly 1033/cm2 s can be produced with existing techniques.

  13. Maternal Psychological Functioning, Family Processes, and Child Adjustment in Rural, Single-Parent, African American Families.

    Science.gov (United States)

    Brody, Gene H.; Flor, Douglas L.

    1997-01-01

    Tested a model linking family financial resources to adjustment among African American 6- to 9-year olds with single, rural, Southern mothers. Found that inadequate financial resources related to mothers' depression and low self-esteem. Self-esteem was linked with family routines and mother-child relationship quality. Child self-regulation…

  14. Phosphatidylserine targeted single-walled carbon nanotubes for photothermal ablation of bladder cancer

    Science.gov (United States)

    Virani, Needa A.; Davis, Carole; McKernan, Patrick; Hauser, Paul; Hurst, Robert E.; Slaton, Joel; Silvy, Ricardo P.; Resasco, Daniel E.; Harrison, Roger G.

    2018-01-01

    Bladder cancer has a 60%-70% recurrence rate most likely due to any residual tumour left behind after a transurethral resection (TUR). Failure to completely resect the cancer can lead to recurrence and progression into higher grade tumours with metastatic potential. We present here a novel therapy to treat superficial tumours with the potential to decrease recurrence. The therapy is a heat-based approach in which bladder tumour specific single-walled carbon nanotubes (SWCNTs) are delivered intravesically at a very low dose (0.1 mg SWCNT per kg body weight) followed 24 h later by a short 30 s treatment with a 360° near-infrared light that heats only the bound nanotubes. The energy density of the treatment was 50 J cm-2, and the power density that this treatment corresponds to is 1.7 W cm-2, which is relatively low. Nanotubes are specifically targeted to the tumour via the interaction of annexin V (AV) and phosphatidylserine, which is normally internalised on healthy tissue but externalised on tumours and the tumour vasculature. SWCNTs are conjugated to AV, which binds specifically to bladder cancer cells as confirmed in vitro and in vivo. Due to this specific localisation, NIR light can be used to heat the tumour while conserving the healthy bladder wall. In a short-term efficacy study in mice with orthotopic MB49 murine bladder tumours treated with the SWCNT-AV conjugate and NIR light, no tumours were visible on the bladder wall 24 h after NIR light treatment, and there was no damage to the bladder. In a separate survival study in mice with the same type of orthotopic tumours, there was a 50% cure rate at 116 days when the study was ended. At 116 days, no treatment toxicity was observed, and no nanotubes were detected in the clearance organs or bladder.

  15. Pulsed laser deposition of chalcogenide sulfides from multi- and single-component targets: the non-stoichiometric material transfer

    DEFF Research Database (Denmark)

    Schou, Jørgen; Ganskukh, Mungunshagai; Ettlinger, Rebecca Bolt

    2018-01-01

    , and the Cu content is also very low at low fluence from a single-component target. Above this threshold, the Cu content in the films increases almost linearly up to a value above the stoichiometric value, while the ratio of the concentration of the other metals Zn to Sn (Zn/Sn) remains constant. Films...

  16. Single-Transverse-Spin-Asymmetry studies with a fixed-target experiment using the LHC beams (AFTER@LHC)

    CERN Document Server

    Lansberg, J.P.; Arnaldi, R.; Brodsky, S.J.; Chambert, V.; Da Silva, C.; Didelez, J.P.; Echevarria, M. G; Ferreiro, E.G.; Fleuret, F.; Gao, Y.; Genolini, B.; Hadjidakis, C.; Hřivnáčová, I.; Kikola, D.; Klein, A.; Kurepin, A.; Kusina, A.; Lorcé, C.; Lyonnet, F.; Massacrier, L.; Nass, A.; Pisano, C.; Robbe, P.; Schienbein, I.; Schlegel, M.; Scomparin, E.; Seixas, J.; Shao, H.S.; Signori, A.; Steffens, E.; Topilskaya, N.; Trzeciak, B.; Uggerhøj, U.I.; Uras, A.; Ulrich, R.; Yang, Z.

    2016-01-01

    We discuss the potential of AFTER@LHC to measure single-transverse-spin asymmetries in open-charm and bottomonium production. With a HERMES-like hydrogen polarised target, such measurements over a year can reach precisions close to the per cent level. This is particularly remarkable since these analyses can probably not be carried out anywhere else

  17. Development and preliminary evaluation of an integrated treatment targeting parenting and depressive symptoms in mothers of children with attention-deficit/hyperactivity disorder.

    Science.gov (United States)

    Chronis-Tuscano, Andrea; Clarke, Tana L; O'Brien, Kelly A; Raggi, Veronica L; Diaz, Yamalis; Mintz, Abigail D; Rooney, Mary E; Knight, Laura A; Seymour, Karen E; Thomas, Sharon R; Seeley, John; Kosty, Derek; Lewinsohn, Peter

    2013-10-01

    More than 50% of mothers of children with attention-deficit/hyperactivity disorder (ADHD) have a lifetime history of major depressive disorder (MDD). Maternal depressive symptoms are associated with impaired parenting and predict adverse developmental and treatment outcomes for children with ADHD. For these reasons, we developed and examined the preliminary efficacy of an integrated treatment targeting parenting and depressive symptoms for mothers of children with ADHD. This integrated intervention incorporated elements of 2 evidence-based treatments: behavioral parent training (BPT) and cognitive behavioral depression treatment. Ninety-eight mothers with at least mild depressive symptoms were randomized to receive either standard BPT (n = 51) or the integrated parenting intervention for ADHD (IPI-A; n = 47). Participants were assessed at baseline, posttreatment, and 3- to 6-month follow-up on measures of (a) self-reported maternal depressive symptoms, (b) observed positive and negative parenting, and (c) observed and mother-reported child disruptive behavior and mother-reported child and family impairment. The IPI-A produced effects of small to moderate magnitude relative to BPT on maternal depressive symptoms, observed negative parenting, observed child deviance, and child impairment at posttreatment and on maternal depressive symptoms, child disruptive behavior, child impairment and family functioning at follow-up. Contrary to expectations, the BPT group demonstrated moderate to large effects relative to IPI-A on observed positive parenting at follow-up. This treatment development study provides encouraging preliminary support for the integrated intervention targeting parenting and depressive symptoms in mothers of children with ADHD. Future studies should examine whether this integrated intervention improves long-term developmental outcomes for children with ADHD. (PsycINFO Database Record (c) 2013 APA, all rights reserved).

  18. The Effect of Incentive Return-to-Work Policies on Single-Parent Families: a Comparative Approach

    Directory of Open Access Journals (Sweden)

    Samir Amine

    2015-10-01

    Full Text Available As part of their struggle against inequality and social exclusion, many countries are trying to reform their redistributive system and low-income support measures to encourage return to work, and reduce inactivity traps. The purpose of this article is to propose a reflection on the social and fiscal policies. The analysis focuses on three measures in three different countries: the Earned Income Tax Credit (EITC in the United States, the Working Tax Credit (WTC in the UK and the Active Solidarity Income (RSA in France. The emphasis is placed on the effects of such policies on the situation of single parents, in particular as part of their transition from welfare (social assistance to the labor market.

  19. Single and double spin asymmetries for deeply virtual Compton scattering measured with CLAS and a longitudinally polarized proton target

    Energy Technology Data Exchange (ETDEWEB)

    Pisano, S.; Biselli, A.; Niccolai, S.; Seder, E.; Guidal, M.; Mirazita, M.; Adhikari, K. P.; Adikaram, D.; Amaryan, M. J.; Anderson, M. D.; Anefalos Pereira, S.; Avakian, H.; Ball, J.; Battaglieri, M.; Batourine, V.; Bedlinskiy, I.; Bosted, P.; Briscoe, B.; Brock, J.; Brooks, W. K.; Burkert, V. D.; Carlin, C.; Carman, D. S.; Celentano, A.; Chandavar, S.; Charles, G.; Colaneri, L.; Cole, P. L.; Compton, N.; Contalbrigo, M.; Cortes, O.; Crabb, D. G.; Crede, V.; D' Angelo, A.; De Vita, R.; De Sanctis, E.; Deur, A.; Djalali, C.; Dupre, R.; Egiyan, H.; El Alaoui, A.; El Fassi, L.; Elouadrhiri, L.; Eugenio, P.; Fedotov, G.; Fegan, S.; Fersch, R.; Filippi, A.; Fleming, J. A.; Fradi, A.; Garillon, B.; Garcon, M.; Ghandilyan, Y.; Gilfoyle, G. P.; Giovanetti, K. L.; Girod, F. X.; Goetz, J. T.; Gohn, W.; Golovatch, E.; Gothe, R. W.; Griffioen, K. A.; Guo, L.; Hafidi, K.; Hanretty, C.; Hattawy, M.; Hicks, K.; Holtrop, M.; Hughes, S. M.; Ilieva, Y.; Ireland, D. G.; Ishkhanov, B. S.; Jenkins, D.; Jiang, X.; Jo, H. S.; Joo, K.; Joosten, S.; Keith, C. D.; Keller, D.; Kim, A.; Kim, W.; Klein, F. J.; Kubarovsky, V.; Kuhn, S. E.; Lenisa, P.; Livingston, K.; Lu, H. Y.; MacCormick, M.; MacGregor, Ian J. D.; Mayer, M.; McKinnon, B.; Meekins, D. G.; Meyer, C. A.; Mokeev, V.; Montgomery, R. A.; Moody, C. I.; Munoz Camacho, C.; Nadel-Turonski, P.; Osipenko, M.; Ostrovidov, A. I.; Park, K.; Phelps, W.; Phillips, J. J.; Pogorelko, O.; Price, J. W.; Procureur, S.; Prok, Y.; Puckett, A. J. R.; Ripani, M.; Rizzo, A.; Rosner, G.; Rossi, P.; Roy, P.; Sabatie, F.; Salgado, C.; Schott, D.; Schumacher, R. A.; Skorodumina, I.; Smith, G. D.; Sober, D. I.; Sokhan, D.; Sparveris, N.; Stepanyan, S.; Stoler, P.; Strauch, S.; Sytnik, V.; Tian, Ye; Tkachenko, S.; Turisini, M.; Ungaro, M.; Voutier, E.; Walford, N. K.; Watts, D. P.; Wei, X.; Weinstein, L. B.; Wood, M. H.; Zachariou, N.; Zana, L.; Zhang, J.; Zhao, Z. W.; Zonta, I.

    2015-03-19

    Single-beam, single-target, and double-spin asymmetries for hard exclusive photon production on the proton e→p→e'p'γ are presented. The data were taken at Jefferson Lab using the CLAS detector and a longitudinally polarized 14NH3 target. The three asymmetries were measured in 165 4-dimensional kinematic bins, covering the widest kinematic range ever explored simultaneously for beam and target-polarization observables in the valence quark region. The kinematic dependences of the obtained asymmetries are discussed and compared to the predictions of models of Generalized Parton Distributions. As a result, the measurement of three DVCS spin observables at the same kinematic points allows a quasi-model-independent extraction of the imaginary parts of the H and H~ Compton Form Factors, which give insight into the electric and axial charge distributions of valence quarks in the proton.

  20. Defending a single object against an attacker trying to detect a subset of false targets

    International Nuclear Information System (INIS)

    Peng, R.; Zhai, Q.Q.; Levitin, G.

    2016-01-01

    Deployment of false targets can be a very important and effective measure for enhancing the survivability of an object subjected to intentional attacks. Existing papers have assumed that false targets are either perfect or can be detected with a constant probability. In practice, the attacker may allocate part of its budget into intelligence actions trying to detect a subset of false targets. Analogously, the defender can allocate part of its budget into disinformation actions to prevent the false targets from being detected. In this paper, the detection probability of each false target is assumed to be a function of the intelligence and disinformation efforts allocated on the false target. The optimal resource distribution between target identification/disinformation and attack/protection efforts is studied as solutions of a non-cooperative two period min–max game between the two competitors for the case of constrained defense and attack resources. - Highlights: • A defense-attack problem is studied as a two-period min–max game. • Both intelligence contest over false targets and impact contest are considered. • Optimal defense and attack strategies are investigated with different parameters.

  1. Targeting single-walled carbon nanotubes for the treatment of breast cancer using photothermal therapy

    Science.gov (United States)

    Neves, Luis Filipe Ferreira

    To develop a therapeutic system with cancer cell selectivity, the present study evaluated a possible specific and localized tumor treatment. Phosphatidylserine (PS) exposure on the external face of the cell membrane is almost completely exclusive to cancer cells and endothelial cells in the tumor vasculature. The human protein annexin V is known to have strong calcium-dependent binding to anionic phospholipids such as PS. This protein was studied for targeting single-walled carbon nanotubes (SWNTs) to the vasculature of breast tumors. The synthesis of the protein annexin V, by a pET vector in Escherichia coli, constitutes the first phase of this study. Recombinant annexin V was purified from the cell lysate supernatant by immobilized metal affinity chromatography. The overall production of purified annexin V protein was 50 mg/L. The binding ability of the protein annexin V was evaluated by determining the dissociation constant when incubated with proliferating human endothelial cells in vitro. The dissociation constant, Kd, was measured to be 0.8 nM, indicating relatively strong binding. This value of Kd is within the range reported in the literature. Single-walled carbon nanotubes (SWNTs) were functionalized with annexin V using two intermediate linkers (containing FMOC and DSPE) resulting in stable suspensions. The SWNT and protein concentrations were 202 mg/L and 515 mg/L, respectively, using the linker with DSPE (average of nine preparations). The conjugation method that used the DSPE-PEG-maleimide linker allowed to successfully conjugate the SWNTs with final concentrations approximately five times higher than the linker containing FMOC. The conjugation method used has a non-covalent nature, and therefore the optical properties of the nanotubes were preserved. The conjugate was also visually observed using atomic force microscopy (AFM), allowing to verify the presence of the protein annexin V on the surface of the nanotubes, with an height ranging between 2

  2. Mother-child discrepancy in perceived parental control and adolescent filial piety in poor single-mother families.

    Science.gov (United States)

    Leung, Janet T Y; Shek, Daniel T L; Lin, Li

    2017-10-01

    Based on a sample of 432 poor Chinese single-mother families (mean age of adolescents = 13.7 years; 51.2% girls; mean age of mothers = 43.5 years) in Hong Kong, the interaction effect of mother-reported and adolescent-reported maternal control on filial piety of Chinese adolescents was examined. Results of polynomial multiple regression analyses showed that the interaction between mother-reported and adolescent-reported maternal control predicted perceived filial piety in adolescents. At high levels of mother-reported maternal control, high adolescent-perceived parental control was associated with higher filial piety. At low levels of mother-reported maternal control, filial piety increased initially and then decreased when adolescents reported higher levels of maternal control. Using multiple group analyses, these associations were found to be stable across gender and age. The present findings provide insights on how congruencies and discrepancies between mother-reported and adolescent-reported maternal control predict filial piety of Chinese adolescents growing up in poor single-mother families. Copyright © 2017 The Foundation for Professionals in Services for Adolescents. Published by Elsevier Ltd. All rights reserved.

  3. Neoliberalism, welfare policy and health: a qualitative meta-synthesis of single parents' experience of the transition from welfare to work.

    Science.gov (United States)

    Cook, Kay

    2012-09-01

    Following the United States' lead, the emergence of neoliberal welfare policy across the western world has resulted in employment programmes for single parents, who are predominantly single mothers. While some governments claim that employment will improve single parents' incomes and well-being, researchers dispute that single parents can unproblematically move into the workforce, with net positive effects. While researchers have quantified the socio-economic effect of these programmes, in particular on participant health, no study has yet synthesized participants' experiences of welfare-to-work. Here, I present a meta-synthesis of eight qualitative health-related studies of single parents' (and exclusively single mothers') welfare-to-work transition. I report that single mothers faced a combination of health and economic issues which made their transition from welfare to work difficult, including degrees of poor physical and mental health. For participants in the United States, these health issues were often compounded by a loss of health benefits on moving into low-wage employment. In countries where a return to employment was required before children reached school age, a lack of affordable and appropriate child care, especially for children with health problems, exacerbated these difficulties. As a result of scarce resources, single mothers in receipt of welfare benefits often relied on food banks or went without food. A return to the workforce did not alleviate this problem as additional child care and reduced government subsidies depleted the funds available for food. I conclude that welfare-to-work policies are underpinned by the neoliberal assumption that the market more efficiently distributes resources than the State. However, for the women in the studies examined here, labour market participation often depleted access to essential resources. Interventions to address the 'problem' of welfare dependency must recognize the complex interplay between work

  4. A Single-Arm Feasibility Trial of Problem-Solving Skills Training for Parents of Children with Idiopathic Chronic Pain Conditions Receiving Intensive Pain Rehabilitation.

    Science.gov (United States)

    Law, Emily F; Fales, Jessica L; Beals-Erickson, Sarah E; Failo, Alessandro; Logan, Deirdre; Randall, Edin; Weiss, Karen; Durkin, Lindsay; Palermo, Tonya M

    2017-05-01

    To adapt problem-solving skills training (PSST) for parents of children receiving intensive pain rehabilitation and evaluate treatment feasibility, acceptability, and satisfaction. Using a prospective single-arm case series design, we evaluated the feasibility of delivering PSST to 26 parents (84.6% female) from one of three pediatric pain rehabilitation programs. Parents completed four to six sessions of PSST delivered during a 2-4-week period. A mixed-methods approach was used to assess treatment acceptability and satisfaction. We also assessed changes in parent mental health and behavior outcomes from pretreatment to immediate posttreatment and 3-month follow-up. Parents demonstrated excellent treatment adherence and rated the intervention as highly acceptable and satisfactory. Preliminary analyses indicated improvements in domains of mental health, parenting behaviors, health status, and problem-solving skills. Findings demonstrate the potential role of psychological interventions directed at reducing parent distress in the context of intensive pediatric pain rehabilitation. © The Author 2016. Published by Oxford University Press on behalf of the Society of Pediatric Psychology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

  5. Differential Impact of a Dutch Alcohol Prevention Program Targeting Adolescents and Parents Separately and Simultaneously: Low Self-Control and Lenient Parenting at Baseline Predict Effectiveness

    NARCIS (Netherlands)

    Koning, I.M.; Verdurmen, J.E.E.; Engels, R.C.M.E.; Eijnden, R.J.J.M. van den; Vollebergh, W.A.M.

    2012-01-01

    To test whether baseline levels of the factors accountable for the impact of the Prevention of Alcohol use in Students (PAS) intervention (self-control, perceived rules about alcohol and parental attitudes about alcohol), moderate the effect of the intervention. A cluster randomized trial including

  6. How Single-Parent Children Speak about Poverty and Social Exclusion: Policy Implications from a Comparative, Qualitative, Cross-National Project

    Science.gov (United States)

    Spyrou, Spyros

    2013-01-01

    This article presents some of the key findings from a comparative, qualitative research study carried out in the United Kingdom, Greece, and Cyprus. The main goal of the study was to investigate single-parent children's experiences and understandings of poverty and social exclusion in their everyday lives and to make relevant policy…

  7. High affinity γPNA sandwich hybridization assay for rapid detection of short nucleic acid targets with single mismatch discrimination.

    Science.gov (United States)

    Goldman, Johnathan M; Zhang, Li Ang; Manna, Arunava; Armitage, Bruce A; Ly, Danith H; Schneider, James W

    2013-07-08

    Hybridization analysis of short DNA and RNA targets presents many challenges for detection. The commonly employed sandwich hybridization approach cannot be implemented for these short targets due to insufficient probe-target binding strengths for unmodified DNA probes. Here, we present a method capable of rapid and stable sandwich hybridization detection for 22 nucleotide DNA and RNA targets. Stable hybridization is achieved using an n-alkylated, polyethylene glycol γ-carbon modified peptide nucleic acid (γPNA) amphiphile. The γPNA's exceptionally high affinity enables stable hybridization of a second DNA-based probe to the remaining bases of the short target. Upon hybridization of both probes, an electrophoretic mobility shift is measured via interaction of the n-alkane modification on the γPNA with capillary electrophoresis running buffer containing nonionic surfactant micelles. We find that sandwich hybridization of both probes is stable under multiple binding configurations and demonstrate single base mismatch discrimination. The binding strength of both probes is also stabilized via coaxial stacking on adjacent hybridization to targets. We conclude with a discussion on the implementation of the proposed sandwich hybridization assay as a high-throughput microRNA detection method.

  8. Structured oligonucleotides for target indexing to allow single-vessel PCR amplification and solid support microarray hybridization.

    Science.gov (United States)

    Girard, Laurie D; Boissinot, Karel; Peytavi, Régis; Boissinot, Maurice; Bergeron, Michel G

    2015-02-07

    The combination of molecular diagnostic technologies is increasingly used to overcome limitations on sensitivity, specificity or multiplexing capabilities, and provide efficient lab-on-chip devices. Two such techniques, PCR amplification and microarray hybridization are used serially to take advantage of the high sensitivity and specificity of the former combined with high multiplexing capacities of the latter. These methods are usually performed in different buffers and reaction chambers. However, these elaborate methods have high complexity and cost related to reagent requirements, liquid storage and the number of reaction chambers to integrate into automated devices. Furthermore, microarray hybridizations have a sequence dependent efficiency not always predictable. In this work, we have developed the concept of a structured oligonucleotide probe which is activated by cleavage from polymerase exonuclease activity. This technology is called SCISSOHR for Structured Cleavage Induced Single-Stranded Oligonucleotide Hybridization Reaction. The SCISSOHR probes enable indexing the target sequence to a tag sequence. The SCISSOHR technology also allows the combination of nucleic acid amplification and microarray hybridization in a single vessel in presence of the PCR buffer only. The SCISSOHR technology uses an amplification probe that is irreversibly modified in presence of the target, releasing a single-stranded DNA tag for microarray hybridization. Each tag is composed of a 3-nucleotide sequence-dependent segment and a unique "target sequence-independent" 14-nucleotide segment allowing for optimal hybridization with minimal cross-hybridization. We evaluated the performance of five (5) PCR buffers to support microarray hybridization, compared to a conventional hybridization buffer. Finally, as a proof of concept, we developed a multiplexed assay for the amplification, detection, and identification of three (3) DNA targets. This new technology will facilitate the design

  9. Single-Spin Asymmetries in Semi-Inclusive Deep-Inelastic Scattering on a Transversely Polarized Hydrogen Target

    Science.gov (United States)

    Airapetian, A.; Akopov, N.; Akopov, Z.; Amarian, M.; Andrus, A.; Aschenauer, E. C.; Augustyniak, W.; Avakian, R.; Avetissian, A.; Avetissian, E.; Bacchetta, A.; Bailey, P.; Balin, D.; Beckmann, M.; Belostotski, S.; Bianchi, N.; Blok, H. P.; Böttcher, H.; Borissov, A.; Borysenko, A.; Bouwhuis, M.; Brüll, A.; Bryzgalov, V.; Capitani, G. P.; Cappiluppi, M.; Chen, T.; Ciullo, G.; Contalbrigo, M.; Dalpiaz, P. F.; Leo, R. De; Demey, M.; Nardo, L. De; Sanctis, E. De; Devitsin, E.; Nezza, P. Di; Düren, M.; Ehrenfried, M.; Elalaoui-Moulay, A.; Elbakian, G.; Ellinghaus, F.; Elschenbroich, U.; Fabbri, R.; Fantoni, A.; Fechtchenko, A.; Felawka, L.; Frullani, S.; Gapienko, G.; Gapienko, V.; Garibaldi, F.; Garrow, K.; Gavrilov, G.; Gharibyan, V.; Grebeniouk, O.; Gregor, I. M.; Hadjidakis, C.; Hafidi, K.; Hartig, M.; Hasch, D.; Henoch, M.; Hesselink, W. H.; Hillenbrand, A.; Hoek, M.; Holler, Y.; Hommez, B.; Hristova, I.; Iarygin, G.; Ilyichev, A.; Ivanilov, A.; Izotov, A.; Jackson, H. E.; Jgoun, A.; Kaiser, R.; Kinney, E.; Kisselev, A.; Kobayashi, T.; Kopytin, M.; Korotkov, V.; Kozlov, V.; Krauss, B.; Krivokhijine, V. G.; Lagamba, L.; Lapikás, L.; Laziev, A.; Lenisa, P.; Liebing, P.; Linden-Levy, L. A.; Lorenzon, W.; Lu, H.; Lu, J.; Lu, S.; Ma, B.-Q.; Maiheu, B.; Makins, N. C.; Mao, Y.; Marianski, B.; Marukyan, H.; Masoli, F.; Mexner, V.; Meyners, N.; Michler, T.; Mikloukho, O.; Miller, C. A.; Miyachi, Y.; Muccifora, V.; Nagaitsev, A.; Nappi, E.; Naryshkin, Y.; Nass, A.; Negodaev, M.; Nowak, W.-D.; Oganessyan, K.; Ohsuga, H.; Osborne, A.; Pickert, N.; Potterveld, D. H.; Raithel, M.; Reggiani, D.; Reimer, P. E.; Reischl, A.; Reolon, A. R.; Riedl, C.; Rith, K.; Rosner, G.; Rostomyan, A.; Rubacek, L.; Rubin, J.; Ryckbosch, D.; Salomatin, Y.; Sanjiev, I.; Savin, I.; Schäfer, A.; Schill, C.; Schnell, G.; Schüler, K. P.; Seele, J.; Seidl, R.; Seitz, B.; Shanidze, R.; Shearer, C.; Shibata, T.-A.; Shutov, V.; Sinram, K.; Sommer, W.; Stancari, M.; Statera, M.; Steffens, E.; Steijger, J. J.; Stenzel, H.; Stewart, J.; Stinzing, F.; Tait, P.; Tanaka, H.; Taroian, S.; Tchuiko, B.; Terkulov, A.; Trzcinski, A.; Tytgat, M.; Vandenbroucke, A.; van der Nat, P. B.; van der Steenhoven, G.; van Haarlem, Y.; Vetterli, M. C.; Vikhrov, V.; Vincter, M. G.; Vogel, C.; Volmer, J.; Wang, S.; Wendland, J.; Wilbert, J.; Smit, G. Ybeles; Ye, Y.; Ye, Z.; Yen, S.; Zihlmann, B.; Zupranski, P.

    2005-01-01

    Single-spin asymmetries for semi-inclusive electroproduction of charged pions in deep-inelastic scattering of positrons are measured for the first time with transverse target polarization. The asymmetry depends on the azimuthal angles of both the pion (ϕ) and the target spin axis (ϕS) about the virtual-photon direction and relative to the lepton scattering plane. The extracted Fourier component πUT is a signal of the previously unmeasured quark transversity distribution, in conjunction with the Collins fragmentation function, also unknown. The component πUT arises from a correlation between the transverse polarization of the target nucleon and the intrinsic transverse momentum of quarks, as represented by the previously unmeasured Sivers distribution function. Evidence for both signals is observed, but the Sivers asymmetry may be affected by exclusive vector meson production.

  10. In-target rare nuclei production rates with EURISOL single-stage configuration

    CERN Document Server

    Chabod, S P; Ene, D; Doré, D; Blideanu, V; David, J.-Ch; Ridikas, D

    2010-01-01

    We conducted calculations of exotic nuclei production rates for 320 configurations of EURISOL (European Isotope Separation On-Line Radioactive Ion Beam Facility) direct spallation targets. The nuclei yields were evaluated using neutron generation-transport codes, completed with evolution calculations to account for nuclei decays and low energy neutron interactions. The yields were optimized for 11 selected elements (Li, Be, Ne, Mg, Ar, Ni, Ga, Kr, Sn, Hg, Fr) and 23 of their isotopes, as function of the target compositions and geometries as well as the incident proton beam energies. For the considered elements, we evaluated the yield distributions as functions of the charge and mass numbers using two different spallation models.

  11. Target-selective homologous recombination cloning for high-throughput generation of monoclonal antibodies from single plasma cells.

    Science.gov (United States)

    Kurosawa, Nobuyuki; Yoshioka, Megumi; Isobe, Masaharu

    2011-04-13

    Molecular cloning of functional immunoglobulin genes from single plasma cells is one of the most promising technologies for the rapid development of monoclonal antibody drugs. However, the proper insertion of PCR-amplified immunoglobulin genes into expression vectors remains an obstacle to the high-throughput production of recombinant monoclonal antibodies. We developed a single-step cloning method, target-selective homologous recombination (TS-HR), in which PCR-amplified immunoglobulin variable genes were selectively inserted into vectors, even in the presence of nonspecifically amplified DNA. TS-HR utilizes Red/ET-mediated homologous recombination with a target-selective vector (TS-vector) with unique homology arms on its termini. Using TS-HR, immunoglobulin variable genes were cloned directly into expression vectors by co-transforming unpurified PCR products and the TS-vector into E. coli. Furthermore, the high cloning specificity of TS-HR allowed plasmids to be extracted from pools of transformed bacteria without screening single colonies for correct clones. We present a one-week protocol for the production of recombinant mouse monoclonal antibodies from large numbers of single plasma cells. The time requirements and limitations of traditional cloning procedures for the production of recombinant immunoglobulins have been significantly reduced with the development of the TS-HR cloning technique. © 2011 Kurosawa et al; licensee BioMed Central Ltd.

  12. Target-selective homologous recombination cloning for high-throughput generation of monoclonal antibodies from single plasma cells

    Directory of Open Access Journals (Sweden)

    Isobe Masaharu

    2011-04-01

    Full Text Available Abstract Background Molecular cloning of functional immunoglobulin genes from single plasma cells is one of the most promising technologies for the rapid development of monoclonal antibody drugs. However, the proper insertion of PCR-amplified immunoglobulin genes into expression vectors remains an obstacle to the high-throughput production of recombinant monoclonal antibodies. Results We developed a single-step cloning method, target-selective homologous recombination (TS-HR, in which PCR-amplified immunoglobulin variable genes were selectively inserted into vectors, even in the presence of nonspecifically amplified DNA. TS-HR utilizes Red/ET-mediated homologous recombination with a target-selective vector (TS-vector with unique homology arms on its termini. Using TS-HR, immunoglobulin variable genes were cloned directly into expression vectors by co-transforming unpurified PCR products and the TS-vector into E. coli. Furthermore, the high cloning specificity of TS-HR allowed plasmids to be extracted from pools of transformed bacteria without screening single colonies for correct clones. We present a one-week protocol for the production of recombinant mouse monoclonal antibodies from large numbers of single plasma cells. Conclusion The time requirements and limitations of traditional cloning procedures for the production of recombinant immunoglobulins have been significantly reduced with the development of the TS-HR cloning technique.

  13. Political violence and child adjustment in Northern Ireland: Testing pathways in a social-ecological model including single-and two-parent families.

    Science.gov (United States)

    Cummings, E Mark; Schermerhorn, Alice C; Merrilees, Christine E; Goeke-Morey, Marcie C; Shirlow, Peter; Cairns, Ed

    2010-07-01

    Moving beyond simply documenting that political violence negatively impacts children, we tested a social-ecological hypothesis for relations between political violence and child outcomes. Participants were 700 mother-child (M = 12.1 years, SD = 1.8) dyads from 18 working-class, socially deprived areas in Belfast, Northern Ireland, including single- and two-parent families. Sectarian community violence was associated with elevated family conflict and children's reduced security about multiple aspects of their social environment (i.e., family, parent-child relations, and community), with links to child adjustment problems and reductions in prosocial behavior. By comparison, and consistent with expectations, links with negative family processes, child regulatory problems, and child outcomes were less consistent for nonsectarian community violence. Support was found for a social-ecological model for relations between political violence and child outcomes among both single- and two-parent families, with evidence that emotional security and adjustment problems were more negatively affected in single-parent families. The implications for understanding social ecologies of political violence and children's functioning are discussed.

  14. Political violence and child adjustment in Northern Ireland: Testing pathways in a social ecological model including single and two-parent families

    Science.gov (United States)

    Cummings, E. Mark; Schermerhorn, Alice C.; Merrilees, Christine E.; Goeke-Morey, Marcie C.; Shirlow, Peter; Cairns, Ed

    2013-01-01

    Moving beyond simply documenting that political violence negatively impacts children, a social ecological hypothesis for relations between political violence and child outcomes was tested. Participants were 700 mother-child (M=12.1years, SD=1.8) dyads from 18 working class, socially deprived areas in Belfast, Northern Ireland, including single- and two-parent families. Sectarian community violence was associated with elevated family conflict and children’s reduced security about multiple aspects of their social environment (i.e., family, parent-child relations, and community), with links to child adjustment problems and reductions in prosocial behavior. By comparison, and consistent with expectations, links with negative family processes, child regulatory problems and child outcomes were less consistent for nonsectarian community violence. Support was found for a social ecological model for relations between political violence and child outcomes among both single and two parent families, with evidence that emotional security and adjustment problems were more negatively affected in single-parent families. The implications for understanding social ecologies of political violence and children’s functioning are discussed. PMID:20604605

  15. A family-based intervention targeting parents of preschool children with overweight and obesity: conceptual framework and study design of LOOPS- Lund overweight and obesity preschool study

    Directory of Open Access Journals (Sweden)

    Önnerfält Jenny

    2012-10-01

    Full Text Available Abstract Background As the rate of overweight among children is rising there is a need for evidence-based research that will clarify what the best interventional strategies to normalize weight development are. The overall aim of the Lund Overweight and Obesity Preschool Study (LOOPS is to evaluate if a family-based intervention, targeting parents of preschool children with overweight and obesity, has a long-term positive effect on weight development of the children. The hypothesis is that preschool children with overweight and obesity, whose parents participate in a one-year intervention, both at completion of the one-year intervention and at long term follow up (2-, 3- and 5-years will have reduced their BMI-for-age z-score. Methods/Design The study is a randomized controlled trial, including overweight (n=160 and obese (n=80 children 4-6-years-old. The intervention is targeting the parents, who get general information about nutrition and exercise recommendations through a website and are invited to participate in a group intervention with the purpose of supporting them to accomplish preferred lifestyle changes, both in the short and long term. To evaluate the effect of various supports, the parents are randomized to different interventions with the main focus of: 1 supporting the parents in limit setting by emphasizing the importance of positive interactions between parents and children and 2 influencing the patterns of daily activities to induce alterations of everyday life that will lead to healthier lifestyle. The primary outcome variable, child BMI-for-age z-score will be measured at referral, inclusion, after 6 months, at the end of intervention and at 2-, 3- and 5-years post intervention. Secondary outcome variables, measured at inclusion and at the end of intervention, are child activity pattern, eating habits and biochemical markers as well as parent BMI, exercise habits, perception of health, experience of parenthood and level of

  16. Revisiting an era in Germany from the perspective of adolescents in mother-headed single-parent families.

    Science.gov (United States)

    Sharma, Deepali; Silbereisen, Rainer K

    2007-02-01

    Much of the documented work on families headed by single mothers is based on empirical evidence from North America and a few Anglo-Saxon countries. Many researchers consider single-mother families to be at a disadvantage because of nonsupportive family policies. This paper uses data from a social context that differed hugely in this respect-the German Democratic Republic (GDR). The GDR provided extensive state support to single-mother families and, thus, was vastly different from other countries. Based on a literature review and using Hill's family stress theory, this paper is based on the hypothesis that adolescents living in mother-headed single-parent families and those living with their biological parents would have near-similar social outcomes (display of psychosomatic symptoms, perception of stress, display of delinquent behaviour, life satisfaction, academic self-efficacy, and academic grades) and family-related outcomes (relationship with parents and perception of family environment). Further, it was hypothesized that adolescents from stepfather families would display more negative social and family-related outcomes when compared with respondents from the other two family types. The sample comprised 1302 adolescents (M  =  13.82 years, SD  =  1.88) who were recruited from the city of Leipzig in the year immediately following German unification, 1991. Respondents reported on measures of psychosomatic symptoms, stress, delinquency, life satisfaction, academic achievement, and family-related variables. ANCOVAs, with a control for income adjusted for household size, indicated adolescents from single-mother families to have very similar experiences to respondents living with their biological parents on all measures except for their assessment of family environment. Adolescents living in stepfather families reported the least favourable experiences. Results are indicative of a social context in Germany that, despite unification and the early hardships for

  17. Targeting a single function of the multifunctional matrix metalloprotease MT1-MMP

    DEFF Research Database (Denmark)

    Ingvarsen, Signe; Porse, Astrid; Erpicum, Charlotte

    2013-01-01

    the enzyme ability to activate proMMP-2 without interfering with the collagenolytic function or the general proteolytic activity of MT1-MMP. Using this antibody, we have shown that the MT1-MMP-catalyzed activation of proMMP-2 is involved in the outgrowth of cultured lymphatic endothelial cells in a collagen......The group of matrix metalloproteases (MMPs) is responsible for multiple processes of extracellular matrix remodeling in the healthy body but also for matrix and tissue destruction during cancer invasion and metastasis. The understanding of the contributions from each individual MMP, both in healthy...... and pathological events, has been complicated by the lack of specific inhibitors and the fact that some of the potent MMPs are multifunctional enzymes. These factors have also hampered the setup of therapeutic strategies targeting MMP activity. A tempting target is the membrane-associated MT1-MMP, which has well...

  18. Successful recruitment strategies for prevention programs targeting children of parents with mental health challenges: An international study

    NARCIS (Netherlands)

    Doesum, K.T.M. van; Riebschleger, J.; Carroll, J.; Grové, C.; Lauritzen, C.; Mordoch, E.; Skerfving, A.

    2016-01-01

    Research substantiates children of parents with mental disorders including substance abuse face increased risk for emotional and behavioral problems. Although evidence suggests that support programs for children enhance resiliency, recruiting children to these groups remains problematic. This study

  19. Target representation of naturalistic echolocation sequences in single unit responses from the inferior colliculus of big brown bats

    Science.gov (United States)

    Sanderson, Mark I.; Simmons, James A.

    2005-11-01

    Echolocating big brown bats (Eptesicus fuscus) emit trains of frequency-modulated (FM) biosonar signals whose duration, repetition rate, and sweep structure change systematically during interception of prey. When stimulated with a 2.5-s sequence of 54 FM pulse-echo pairs that mimic sounds received during search, approach, and terminal stages of pursuit, single neurons (N=116) in the bat's inferior colliculus (IC) register the occurrence of a pulse or echo with an average of <1 spike/sound. Individual IC neurons typically respond to only a segment of the search or approach stage of pursuit, with fewer neurons persisting to respond in the terminal stage. Composite peristimulus-time-histogram plots of responses assembled across the whole recorded population of IC neurons depict the delay of echoes and, hence, the existence and distance of the simulated biosonar target, entirely as on-response latencies distributed across time. Correlated changes in pulse duration, repetition rate, and pulse or echo amplitude do modulate the strength of responses (probability of the single spike actually occurring for each sound), but registration of the target itself remains confined exclusively to the latencies of single spikes across cells. Modeling of echo processing in FM biosonar should emphasize spike-time algorithms to explain the content of biosonar images.

  20. Building a Robust Tumor Profiling Program: Synergy between Next-Generation Sequencing and Targeted Single-Gene Testing.

    Directory of Open Access Journals (Sweden)

    Matthew C Hiemenz

    Full Text Available Next-generation sequencing (NGS is a powerful platform for identifying cancer mutations. Routine clinical adoption of NGS requires optimized quality control metrics to ensure accurate results. To assess the robustness of our clinical NGS pipeline, we analyzed the results of 304 solid tumor and hematologic malignancy specimens tested simultaneously by NGS and one or more targeted single-gene tests (EGFR, KRAS, BRAF, NPM1, FLT3, and JAK2. For samples that passed our validated tumor percentage and DNA quality and quantity thresholds, there was perfect concordance between NGS and targeted single-gene tests with the exception of two FLT3 internal tandem duplications that fell below the stringent pre-established reporting threshold but were readily detected by manual inspection. In addition, NGS identified clinically significant mutations not covered by single-gene tests. These findings confirm NGS as a reliable platform for routine clinical use when appropriate quality control metrics, such as tumor percentage and DNA quality cutoffs, are in place. Based on our findings, we suggest a simple workflow that should facilitate adoption of clinical oncologic NGS services at other institutions.

  1. Building a Robust Tumor Profiling Program: Synergy between Next-Generation Sequencing and Targeted Single-Gene Testing.

    Science.gov (United States)

    Hiemenz, Matthew C; Kadauke, Stephan; Lieberman, David B; Roth, David B; Zhao, Jianhua; Watt, Christopher D; Daber, Robert D; Morrissette, Jennifer J D

    2016-01-01

    Next-generation sequencing (NGS) is a powerful platform for identifying cancer mutations. Routine clinical adoption of NGS requires optimized quality control metrics to ensure accurate results. To assess the robustness of our clinical NGS pipeline, we analyzed the results of 304 solid tumor and hematologic malignancy specimens tested simultaneously by NGS and one or more targeted single-gene tests (EGFR, KRAS, BRAF, NPM1, FLT3, and JAK2). For samples that passed our validated tumor percentage and DNA quality and quantity thresholds, there was perfect concordance between NGS and targeted single-gene tests with the exception of two FLT3 internal tandem duplications that fell below the stringent pre-established reporting threshold but were readily detected by manual inspection. In addition, NGS identified clinically significant mutations not covered by single-gene tests. These findings confirm NGS as a reliable platform for routine clinical use when appropriate quality control metrics, such as tumor percentage and DNA quality cutoffs, are in place. Based on our findings, we suggest a simple workflow that should facilitate adoption of clinical oncologic NGS services at other institutions.

  2. Circular revisit orbits design for responsive mission over a single target

    Science.gov (United States)

    Li, Taibo; Xiang, Junhua; Wang, Zhaokui; Zhang, Yulin

    2016-10-01

    The responsive orbits play a key role in addressing the mission of Operationally Responsive Space (ORS) because of their capabilities. These capabilities are usually focused on supporting specific targets as opposed to providing global coverage. One subtype of responsive orbits is repeat coverage orbit which is nearly circular in most remote sensing applications. This paper deals with a special kind of repeating ground track orbit, referred to as circular revisit orbit. Different from traditional repeat coverage orbits, a satellite on circular revisit orbit can visit a target site at both the ascending and descending stages in one revisit cycle. This typology of trajectory allows a halving of the traditional revisit time and does a favor to get useful information for responsive applications. However the previous reported numerical methods in some references often cost lots of computation or fail to obtain such orbits. To overcome this difficulty, an analytical method to determine the existence conditions of the solutions to revisit orbits is presented in this paper. To this end, the mathematical model of circular revisit orbit is established under the central gravity model and the J2 perturbation. A constraint function of the circular revisit orbit is introduced, and the monotonicity of that function has been studied. The existent conditions and the number of such orbits are naturally worked out. Taking the launch cost into consideration, optimal design model of circular revisit orbit is established to achieve a best orbit which visits a target twice a day in the morning and in the afternoon respectively for several days. The result shows that it is effective to apply circular revisit orbits in responsive application such as reconnoiter of natural disaster.

  3. The single lineup paradigm: A new way to manipulate target presence in eyewitness identification experiments.

    Science.gov (United States)

    Oriet, Chris; Fitzgerald, Ryan J

    2018-02-01

    The suspect in eyewitness lineups may be guilty or innocent. These possibilities are traditionally simulated in eyewitness identification studies using a dual-lineup paradigm: All witnesses observe the same perpetrator and then receive one of two lineups. In this paradigm, the suspect's guilt is manipulated by including the perpetrator in one lineup and an innocent suspect in the other. The lineup is then filled with people matched to either the suspect (resulting in different fillers in perpetrator-present and perpetrator-absent lineups) or to the perpetrator (resulting in the same fillers in each lineup). An inescapable feature of the dual-lineup paradigm is that the perpetrator-present and perpetrator-absent lineups differ not only in the suspect's guilt, but also in their composition. Here, we describe a single-lineup paradigm: Subjects observe one of two perpetrators and then all subjects receive the same lineup containing one of the perpetrators. This alternative paradigm allows manipulation of the suspect's guilt without changing the lineup's composition. In three experiments, we applied the single-lineup paradigm to explore suspect-filler similarity and consistently found that increasing similarity reduced perpetrator identifications but did little to prevent innocent suspect misidentifications. Conversely, when fillers were matched to the perpetrator using a dual-lineup paradigm, increasing similarity reduced identification of perpetrators and innocent suspects. This finding suggests that the effect of filler similarity may depend on the person to whom the fillers are matched. We suggest that the single-lineup paradigm is a more ecologically valid and better controlled approach to creating suspect-matched lineups in laboratory investigations of eyewitness memory than existing procedures. (PsycINFO Database Record (c) 2018 APA, all rights reserved).

  4. Construction from a single parent of baker's yeast strains with high freeze tolerance and fermentative activity in both lean and sweet doughs.

    Science.gov (United States)

    Nakagawa, S; Ouchi, K

    1994-10-01

    From a freeze-tolerant baker's yeast (Saccharomyces cerevisiae), 2,333 spore clones were obtained. To improve the leavening ability in lean dough of the parent strain, we selected 555 of the high-maltose-fermentative spore clones by using a method in which a soft agar solution containing maltose and bromocresol purple was overlaid on yeast colonies. By measuring the gassing power in the dough, we selected 66 spore clones with a good leavening ability in lean dough and a total of 694 hybrids were constructed by crossing them. Among these hybrids, we obtained 50 novel freeze-tolerant strains with good leavening ability in all lean, regular, and sweet doughs comparable to that of commercial baker's yeast. Hybrids with improved leavening ability or freeze tolerance compared with the parent yeast and commercial baker's yeasts were also obtained. These results suggest that hybridization between spore clones derived from a single parent strain is effective for improving the properties of baker's yeasts.

  5. Joint synthetic aperture radar plus ground moving target indicator from single-channel radar using compressive sensing

    Energy Technology Data Exchange (ETDEWEB)

    Thompson, Douglas; Hallquist, Aaron; Anderson, Hyrum

    2017-10-17

    The various embodiments presented herein relate to utilizing an operational single-channel radar to collect and process synthetic aperture radar (SAR) and ground moving target indicator (GMTI) imagery from a same set of radar returns. In an embodiment, data is collected by randomly staggering a slow-time pulse repetition interval (PRI) over a SAR aperture such that a number of transmitted pulses in the SAR aperture is preserved with respect to standard SAR, but many of the pulses are spaced very closely enabling movers (e.g., targets) to be resolved, wherein a relative velocity of the movers places them outside of the SAR ground patch. The various embodiments of image reconstruction can be based on compressed sensing inversion from undersampled data, which can be solved efficiently using such techniques as Bregman iteration. The various embodiments enable high-quality SAR reconstruction, and high-quality GMTI reconstruction from the same set of radar returns.

  6. Target-mediated drug disposition with drug-drug interaction, Part I: single drug case in alternative formulations.

    Science.gov (United States)

    Koch, Gilbert; Jusko, William J; Schropp, Johannes

    2017-02-01

    Target-mediated drug disposition (TMDD) describes drug binding with high affinity to a target such as a receptor. In application TMDD models are often over-parameterized and quasi-equilibrium (QE) or quasi-steady state (QSS) approximations are essential to reduce the number of parameters. However, implementation of such approximations becomes difficult for TMDD models with drug-drug interaction (DDI) mechanisms. Hence, alternative but equivalent formulations are necessary for QE or QSS approximations. To introduce and develop such formulations, the single drug case is reanalyzed. This work opens the route for straightforward implementation of QE or QSS approximations of DDI TMDD models. The manuscript is the first part to introduce DDI TMDD models with QE or QSS approximations.

  7. The “curved lead pathway” method to enable a single lead to reach any two intracranial targets

    Science.gov (United States)

    Ding, Chen-Yu; Yu, Liang-Hong; Lin, Yuan-Xiang; Chen, Fan; Lin, Zhang-Ya; Kang, De-Zhi

    2017-01-01

    Deep brain stimulation is an effective way to treat movement disorders, and a powerful research tool for exploring brain functions. This report proposes a “curved lead pathway” method for lead implantation, such that a single lead can reach in sequence to any two intracranial targets. A new type of stereotaxic system for implanting a curved lead to the brain of human/primates was designed, the auxiliary device needed for this method to be used in rat/mouse was fabricated and verified in rat, and the Excel algorithm used for automatically calculating the necessary parameters was implemented. This “curved lead pathway” method of lead implantation may complement the current method, make lead implantation for multiple targets more convenient, and expand the experimental techniques of brain function research.

  8. Road-Aided Ground Slowly Moving Target 2D Motion Estimation for Single-Channel Synthetic Aperture Radar

    Directory of Open Access Journals (Sweden)

    Zhirui Wang

    2016-03-01

    Full Text Available To detect and estimate ground slowly moving targets in airborne single-channel synthetic aperture radar (SAR, a road-aided ground moving target indication (GMTI algorithm is proposed in this paper. First, the road area is extracted from a focused SAR image based on radar vision. Second, after stationary clutter suppression in the range-Doppler domain, a moving target is detected and located in the image domain via the watershed method. The target’s position on the road as well as its radial velocity can be determined according to the target’s offset distance and traffic rules. Furthermore, the target’s azimuth velocity is estimated based on the road slope obtained via polynomial fitting. Compared with the traditional algorithms, the proposed method can effectively cope with slowly moving targets partly submerged in a stationary clutter spectrum. In addition, the proposed method can be easily extended to a multi-channel system to further improve the performance of clutter suppression and motion estimation. Finally, the results of numerical experiments are provided to demonstrate the effectiveness of the proposed algorithm.

  9. Genetically engineered T cells bearing chimeric nanoconstructed receptors harboring TAG-72-specific camelid single domain antibodies as targeting agents

    DEFF Research Database (Denmark)

    Sharifzadeh, Zahra; Rahbarizadeh, Fatemeh; Shokrgozar, Mohammad A

    2013-01-01

    Despite the preclinical success of adoptive therapy with T cells bearing chimeric nanoconstructed antigen receptors (CARs), certain limitations of this therapeutic approach such as the immunogenicity of the antigen binding domain, the emergence of tumor cell escape variants and the blocking...... expressing tumor cells, the combination of CD3ζ, OX40, CD28 as well as the CH3-CH2-hinge-hinge domains most efficiently triggered T cell activation. Importantly, CAR mediated functions were not blocked by the soluble TAG-72 antigen at a supraphysiological concentration. Our approach may have the potential...... capacity of soluble antigen still remain. Here, we address these issues using a novel CAR binding moiety based on the oligoclonal camelid single domain antibodies. A unique set of 13 single domain antibodies were selected from an immunized camel phage library based on their target specificity and binding...

  10. Predictive Biomarkers in Colorectal Cancer: From the Single Therapeutic Target to a Plethora of Options

    Directory of Open Access Journals (Sweden)

    Daniela Rodrigues

    2016-01-01

    Full Text Available Colorectal cancer (CRC is one of the most frequent cancers and is a leading cause of cancer death worldwide. Treatments used for CRC may include some combination of surgery, radiation therapy, chemotherapy, and targeted therapy. The current standard drugs used in chemotherapy are 5-fluorouracil and leucovorin in combination with irinotecan and/or oxaliplatin. Most recently, biologic agents have been proven to have therapeutic benefits in metastatic CRC alone or in association with standard chemotherapy. However, patients present different treatment responses, in terms of efficacy and toxicity; therefore, it is important to identify biological markers that can predict the response to therapy and help select patients that would benefit from specific regimens. In this paper, authors review CRC genetic markers that could be useful in predicting the sensitivity/resistance to chemotherapy.

  11. Targeted physiotherapy for patellofemoral joint osteoarthritis: A protocol for a randomised, single-blind controlled trial

    Directory of Open Access Journals (Sweden)

    Schache Anthony G

    2008-09-01

    Full Text Available Abstract Background The patellofemoral joint (PFJ is one compartment of the knee that is frequently affected by osteoarthritis (OA and is a potent source of OA symptoms. However, there is a dearth of evidence for compartment-specific treatments for PFJ OA. Therefore, this project aims to evaluate whether a physiotherapy treatment, targeted to the PFJ, results in greater improvements in pain and physical function than a physiotherapy education intervention in people with symptomatic and radiographic PFJ OA. Methods 90 people with PFJ OA (PFJ-specific history, signs and symptoms and radiographic evidence of PFJ OA will be recruited from the community and randomly allocated into one of two treatments. A randomised controlled trial adhering to CONSORT guidelines will evaluate the efficacy of physiotherapy (8 individual sessions over 12 weeks, as well as a home exercise program 4 times/week compared to a physiotherapist-delivered OA education control treatment (8 individual sessions over 12 weeks. Physiotherapy treatment will consist of (i quadriceps muscle retraining; (ii quadriceps and hip muscle strengthening; (iii patellar taping; (iv manual PFJ and soft tissue mobilisation; and (v OA education. Resistance and dosage of exercises will be tailored to the participant's functional level and clinical state. Primary outcomes will be evaluated by a blinded examiner at baseline, 12 weeks and 9 months using validated and reliable pain, physical function and perceived global effect scales. All analyses will be conducted on an intention-to-treat basis using linear mixed regression models, including respective baseline scores as a covariate, subjects as a random effect, treatment condition as a fixed factor and the covariate by treatment interaction. Conclusion This RCT is targeting PFJ OA, an important sub-group of knee OA patients, with a specifically designed conservative intervention. The project's outcome will influence PFJ OA rehabilitation, with the

  12. Targeted physiotherapy for patellofemoral joint osteoarthritis: A protocol for a randomised, single-blind controlled trial

    Science.gov (United States)

    Crossley, Kay M; Vicenzino, Bill; Pandy, Marcus G; Schache, Anthony G; Hinman, Rana S

    2008-01-01

    Background The patellofemoral joint (PFJ) is one compartment of the knee that is frequently affected by osteoarthritis (OA) and is a potent source of OA symptoms. However, there is a dearth of evidence for compartment-specific treatments for PFJ OA. Therefore, this project aims to evaluate whether a physiotherapy treatment, targeted to the PFJ, results in greater improvements in pain and physical function than a physiotherapy education intervention in people with symptomatic and radiographic PFJ OA. Methods 90 people with PFJ OA (PFJ-specific history, signs and symptoms and radiographic evidence of PFJ OA) will be recruited from the community and randomly allocated into one of two treatments. A randomised controlled trial adhering to CONSORT guidelines will evaluate the efficacy of physiotherapy (8 individual sessions over 12 weeks, as well as a home exercise program 4 times/week) compared to a physiotherapist-delivered OA education control treatment (8 individual sessions over 12 weeks). Physiotherapy treatment will consist of (i) quadriceps muscle retraining; (ii) quadriceps and hip muscle strengthening; (iii) patellar taping; (iv) manual PFJ and soft tissue mobilisation; and (v) OA education. Resistance and dosage of exercises will be tailored to the participant's functional level and clinical state. Primary outcomes will be evaluated by a blinded examiner at baseline, 12 weeks and 9 months using validated and reliable pain, physical function and perceived global effect scales. All analyses will be conducted on an intention-to-treat basis using linear mixed regression models, including respective baseline scores as a covariate, subjects as a random effect, treatment condition as a fixed factor and the covariate by treatment interaction. Conclusion This RCT is targeting PFJ OA, an important sub-group of knee OA patients, with a specifically designed conservative intervention. The project's outcome will influence PFJ OA rehabilitation, with the potential to reduce

  13. Single-well tracer methods for hydrogeologic evaluation of target aquifers

    International Nuclear Information System (INIS)

    Hall, S.H.

    1994-11-01

    Designing an efficient well field for an aquifer thermal energy storage (ATES) project requires measuring local groundwater flow parameters as well as estimating horizontal and vertical inhomogeneity. Effective porosity determines the volume of aquifer needed to store a given volume of heated or chilled water. Ground-water flow velocity governs the migration of the thermal plume, and dispersion and heat exchange along the flow path reduces the thermal intensity of the recovered plume. Stratigraphic variations in the aquifer will affect plume dispersion, may bias the apparent rate of migration of the plume, and can prevent efficient hydraulic communication between wells. Single-well tracer methods using a conservative flow tracer such as bromide, along with pumping tests and water-level measurements, provide a rapid and cost-effective means for estimating flow parameters. A drift-and-pumpback tracer test yields effective porosity and flow velocity. Point-dilution tracer testing, using new instrumentation for downhole tracer measurement and a new method for calibrating the point-dilution test itself, yields depth-discrete hydraulic conductivity as it is affected by stratigraphy, and can be used to estimate well transmissivity. Experience in conducting both drift-and-pumpback and point-dilution tests at three different test sites has yielded important information that highlights both the power and the limitations of the single-well tracer methods. These sites are the University of Alabama Student Recreation Center (UASRC) ATES well field and the VA Medical Center (VA) ATES well field, both located in Tuscaloosa, Alabama, and the Hanford bioremediation test site north of Richland, Washington

  14. Inter- and Intrafraction Target Motion in Highly Focused Single Vocal Cord Irradiation of T1a Larynx Cancer Patients

    Energy Technology Data Exchange (ETDEWEB)

    Kwa, Stefan L.S., E-mail: s.kwa@erasmusmc.nl; Al-Mamgani, Abrahim; Osman, Sarah O.S.; Gangsaas, Anne; Levendag, Peter C.; Heijmen, Ben J.M.

    2015-09-01

    Purpose: The purpose of this study was to verify clinical target volume–planning target volume (CTV-PTV) margins in single vocal cord irradiation (SVCI) of T1a larynx tumors and characterize inter- and intrafraction target motion. Methods and Materials: For 42 patients, a single vocal cord was irradiated using intensity modulated radiation therapy at a total dose of 58.1 Gy (16 fractions × 3.63 Gy). A daily cone beam computed tomography (CBCT) scan was performed to online correct the setup of the thyroid cartilage after patient positioning with in-room lasers (interfraction motion correction). To monitor intrafraction motion, CBCT scans were also acquired just after patient repositioning and after dose delivery. A mixed online-offline setup correction protocol (“O2 protocol”) was designed to compensate for both inter- and intrafraction motion. Results: Observed interfraction, systematic (Σ), and random (σ) setup errors in left-right (LR), craniocaudal (CC), and anteroposterior (AP) directions were 0.9, 2.0, and 1.1 mm and 1.0, 1.6, and 1.0 mm, respectively. After correction of these errors, the following intrafraction movements derived from the CBCT acquired after dose delivery were: Σ = 0.4, 1.3, and 0.7 mm, and σ = 0.8, 1.4, and 0.8 mm. More than half of the patients showed a systematic non-zero intrafraction shift in target position, (ie, the mean intrafraction displacement over the treatment fractions was statistically significantly different from zero; P<.05). With the applied CTV-PTV margins (for most patients 3, 5, and 3 mm in LR, CC, and AP directions, respectively), the minimum CTV dose, estimated from the target displacements observed in the last CBCT, was at least 94% of the prescribed dose for all patients and more than 98% for most patients (37 of 42). The proposed O2 protocol could effectively reduce the systematic intrafraction errors observed after dose delivery to almost zero (Σ = 0.1, 0.2, 0.2 mm). Conclusions: With

  15. Improving support for parents of children with hearing loss: provider training on use of targeted communication strategies.

    Science.gov (United States)

    Muñoz, Karen; Nelson, Lauri; Blaiser, Kristina; Price, Tanner; Twohig, Michael

    2015-02-01

    When proper protocols are followed, children who are identified with a permanent hearing loss early in life have opportunities to develop language on par with their typical hearing peers. Young children with hearing loss are dependent on their parents to manage intervention during early years critical to their development, and parents' ability to effectively integrate recommendations in daily life is foundational for intervention success. Audiologists and early intervention professionals not only need to provide current evidence-based services, but also must address parents' emotional and learning needs related to their child's hearing loss. This study explored practice patterns related to education and support provided to parents of children with hearing loss and the influence of an in-service training on provider attitudes. This study used a prepost design with a self-report questionnaire to identify practice patterns related to communication skills and support used by providers when working with parents of children with hearing loss. A total of 45 participants (21 professionals and 24 graduate students) currently working with children completed the pretraining questionnaire, and 29 participants (13 professionals and 16 graduate students) completed the postquestionnaire. Data were collected using an online questionnaire before the training and 1 mo after training. Descriptive analyses were done to identify trends, and paired-samples t-tests were used to determine changes pretraining to posttraining. Findings revealed that professionals most frequently teach skills to mothers (91%) and infrequently teach skills to fathers (19%) and other caregivers (10%). Professionals reported frequently collaborating with other intervention providers (76%) and infrequently collaborating with primary care physicians (19%). One-third of the professionals reported addressing symptoms of depression and anxiety as an interfering factor with the ability to implement management

  16. Semi-Inclusive Single Pion Electroproduction From Deuteron and Proton Targets

    Energy Technology Data Exchange (ETDEWEB)

    Kalantarians, Narbe [Univ. of Houston, TX (United States)

    2008-08-01

    Semi-inclusive electroproduction of all three pion flavors (π+, π-0) from both deuteron and proton targets is measured for Q^2 > 1.1 GeV2, 0.12 < x < 0.48, 0.3 < z < 0.7. and Mx > 1.4 GeV. We study the PT, Z, and Φh dependences for the ratio of yields d(e,e',π)X/p(e,e'π)X. From these ratios it is possible to study the d/u valence quark distributions for the neutron and proton as well as the ratio of (current) fragmentation functions D-/D+. The data presented is from Jefferson Lab experiment PR94-102, using an electron beam with energy 5.769 GeV with beam current of 7nA. The results agree with previous data as well as provide an extended kinematic coverage.

  17. Therapeutic Effect of Novel Single-Stranded RNAi Agent Targeting Periostin in Eyes with Retinal Neovascularization

    Directory of Open Access Journals (Sweden)

    Takahito Nakama

    2017-03-01

    Full Text Available Retinal neovascularization (NV due to retinal ischemia remains one of the principal causes of vision impairment in patients with ischemic retinal diseases. We recently reported that periostin (POSTN may play a role in the development of preretinal fibrovascular membranes, but its role in retinal NV has not been determined. The purpose of this study was to examine the expression of POSTN in the ischemic retinas of a mouse model of oxygen-induced retinal NV. We also studied the function of POSTN on retinal NV using Postn KO mice and human retinal endothelial cells (HRECs in culture. In addition, we used a novel RNAi agent, NK0144, which targets POSTN to determine its effect on the development of retinal NV. Our results showed that the expression of POSTN was increased in the vascular endothelial cells, pericytes, and M2 macrophages in ischemic retinas. POSTN promoted the ischemia-induced retinal NV by Akt phosphorylation through integrin αvβ3. NK0144 had a greater inhibitory effect than canonical double-stranded siRNA on preretinal pathological NV in vivo and in vitro. These findings suggest a causal relationship between POSTN and retinal NV, and indicate a potential therapeutic role of intravitreal injection of NK0144 for retinal neovascular diseases.

  18. Targeting Aging with Functional Food: Pasta with Opuntia Single-Arm Pilot Study.

    Science.gov (United States)

    Aiello, Anna; Di Bona, Danilo; Candore, Giuseppina; Carru, Ciriaco; Zinellu, Angelo; Di Miceli, Giuseppe; Nicosia, Aldo; Gambino, Caterina Maria; Ruisi, Paolo; Caruso, Calogero; Vasto, Sonya; Accardi, Giulia

    2017-10-10

    Interventions to extend life span represent the new perspective in aging investigation. Healthy dietary habits are important modifiable factors that can favor a healthy aging phenotype. Many studies have demonstrated benefits for metabolic syndrome and type 2 diabetes mellitus resulting from the traditional Mediterranean foods. Opuntia Ficus Indica (OFI), widespread in the Mediterranean basin, belongs to the Cactaceae family. It is known for its antioxidant and anti-inflammatory properties. Moreover, products containing extracts from OFI fruits or cladodes have been used to control obesity and other metabolic parameters, such as glycemia and lipid profile. The aim of this study was to analyze the antioxidant and anti-inflammatory effect of pasta with 3% of OFI cladode extracts added to show its beneficial effect in human health. We performed a single arm longitudinal intervention study in 42 healthy volunteers, administrating 500 g/week of this functional pasta for 30 days. Our pasta had antioxidant and anti-inflammatory properties with putative effect on the aging process and related metabolic diseases. We also demonstrated a hypoglycemic effect. The results are preliminary, but it is possible to speculate that our pasta could be considered an effective food for the prevention of age-related metabolic disorders.

  19. Parental factors associated with screen time in pre-school children in primary-care practice: a TARGet Kids! study.

    Science.gov (United States)

    Birken, Catherine S; Maguire, Jonathon; Mekky, Magda; Manlhiot, Cedric; Beck, Carolyn E; Jacobson, Sheila; Peer, Michael; Taylor, Carolyn; McCrindle, Brian W; Parkin, Patricia C

    2011-12-01

    To identify child and parental factors associated with screen time in 3-year-old children. Observational study. Participants were recruited from a large primary-care paediatric group practice in Toronto, Canada. Healthy 3-year-old children were included. A questionnaire was completed by their parents on screen time. Descriptive statistics and linear regression models were used to assess associations between child screen time and selected factors. Multivariable models included factors from the univariate analysis with P eating lunch and dinner in front of the screen and mother being employed were associated with an increase in child weekday screen time of 96 (95% CI 30, 192), 42 (95% CI 12, 90) and 36 (95% CI 6, 72) min/d, respectively. Eating lunch in front of the screen and an increase of 1 h of parental screen time were associated with an increase of 78 (95% CI 36, 132) and 12 (95% CI 6, 18) min/d in child weekend screen time. Family rules decreased child weekend screen time by 30 (95% CI 6, 54) min/d. Interventions that include these important parental factors should be evaluated for their effectiveness in reducing screen time.

  20. Functionalized single-walled carbon nanotube-based fuel cell benchmarked against US DOE 2017 technical targets.

    Science.gov (United States)

    Jha, Neetu; Ramesh, Palanisamy; Bekyarova, Elena; Tian, Xiaojuan; Wang, Feihu; Itkis, Mikhail E; Haddon, Robert C

    2013-01-01

    Chemically modified single-walled carbon nanotubes (SWNTs) with varying degrees of functionalization were utilized for the fabrication of SWNT thin film catalyst support layers (CSLs) in polymer electrolyte membrane fuel cells (PEMFCs), which were suitable for benchmarking against the US DOE 2017 targets. Use of the optimum level of SWNT -COOH functionality allowed the construction of a prototype SWNT-based PEMFC with total Pt loading of 0.06 mg(Pt)/cm²--well below the value of 0.125 mg(Pt)/cm² set as the US DOE 2017 technical target for total Pt group metals (PGM) loading. This prototype PEMFC also approaches the technical target for the total Pt content per kW of power (<0.125 g(PGM)/kW) at cell potential 0.65 V: a value of 0.15 g(Pt)/kW was achieved at 80°C/22 psig testing conditions, which was further reduced to 0.12 g(Pt)/kW at 35 psig back pressure.

  1. Accurate mitochondrial DNA sequencing using off-target reads provides a single test to identify pathogenic point mutations.

    Science.gov (United States)

    Griffin, Helen R; Pyle, Angela; Blakely, Emma L; Alston, Charlotte L; Duff, Jennifer; Hudson, Gavin; Horvath, Rita; Wilson, Ian J; Santibanez-Koref, Mauro; Taylor, Robert W; Chinnery, Patrick F

    2014-12-01

    Mitochondrial disorders are a common cause of inherited metabolic disease and can be due to mutations affecting mitochondrial DNA or nuclear DNA. The current diagnostic approach involves the targeted resequencing of mitochondrial DNA and candidate nuclear genes, usually proceeds step by step, and is time consuming and costly. Recent evidence suggests that variations in mitochondrial DNA sequence can be obtained from whole-exome sequence data, raising the possibility of a comprehensive single diagnostic test to detect pathogenic point mutations. We compared the mitochondrial DNA sequence derived from off-target exome reads with conventional mitochondrial DNA Sanger sequencing in 46 subjects. Mitochondrial DNA sequences can be reliably obtained using three different whole-exome sequence capture kits. Coverage correlates with the relative amount of mitochondrial DNA in the original genomic DNA sample, heteroplasmy levels can be determined using variant and total read depths, and-providing there is a minimum read depth of 20-fold-rare sequencing errors occur at a rate similar to that observed with conventional Sanger sequencing. This offers the prospect of using whole-exome sequence in a diagnostic setting to screen not only all protein coding nuclear genes but also all mitochondrial DNA genes for pathogenic mutations. Off-target mitochondrial DNA reads can also be used to assess quality control and maternal ancestry, inform on ethnic origin, and allow genetic disease association studies not previously anticipated with existing whole-exome data sets.

  2. Single-molecule motions and interactions in live cells reveal target search dynamics in mismatch repair.

    Science.gov (United States)

    Liao, Yi; Schroeder, Jeremy W; Gao, Burke; Simmons, Lyle A; Biteen, Julie S

    2015-12-15

    MutS is responsible for initiating the correction of DNA replication errors. To understand how MutS searches for and identifies rare base-pair mismatches, we characterized the dynamic movement of MutS and the replisome in real time using superresolution microscopy and single-molecule tracking in living cells. We report that MutS dynamics are heterogeneous in cells, with one MutS population exploring the nucleoid rapidly, while another MutS population moves to and transiently dwells at the replisome region, even in the absence of appreciable mismatch formation. Analysis of MutS motion shows that the speed of MutS is correlated with its separation distance from the replisome and that MutS motion slows when it enters the replisome region. We also show that mismatch detection increases MutS speed, supporting the model for MutS sliding clamp formation after mismatch recognition. Using variants of MutS and the replication processivity clamp to impair mismatch repair, we find that MutS dynamically moves to and from the replisome before mismatch binding to scan for errors. Furthermore, a block to DNA synthesis shows that MutS is only capable of binding mismatches near the replisome. It is well-established that MutS engages in an ATPase cycle, which is necessary for signaling downstream events. We show that a variant of MutS with a nucleotide binding defect is no longer capable of dynamic movement to and from the replisome, showing that proper nucleotide binding is critical for MutS to localize to the replisome in vivo. Our results provide mechanistic insight into the trafficking and movement of MutS in live cells as it searches for mismatches.

  3. Octa-ammonium POSS-conjugated single-walled carbon nanotubes as vehicles for targeted delivery of paclitaxel

    Directory of Open Access Journals (Sweden)

    Naghmeh Naderi

    2015-09-01

    Full Text Available Background: Carbon nanotubes (CNTs have unique physical and chemical properties. Furthermore, novel properties can be developed by attachment or encapsulation of functional groups. These unique properties facilitate the use of CNTs in drug delivery. We developed a new nanomedicine consisting of a nanocarrier, cell-targeting molecule, and chemotherapeutic drug and assessed its efficacy in vitro. Methods: The efficacy of a single-walled carbon nanotubes (SWCNTs-based nanoconjugate system is assessed in the targeted delivery of paclitaxel (PTX to cancer cells. SWCNTs were oxidized and reacted with octa-ammonium polyhedral oligomeric silsesquioxanes (octa-ammonium POSS to render them biocompatible and water dispersable. The functionalized SWCNTs were loaded with PTX, a chemotherapeutic agent toxic to cancer cells, and Tn218 antibodies for cancer cell targeting. The nanohybrid composites were characterized with transmission electron microscopy (TEM, Fourier transform infrared (FTIR, and ultraviolet–visible–near-infrared (UV–Vis–NIR. Additionally, their cytotoxic effects on Colon cancer cell (HT-29 and Breast cancer cell (MCF-7 lines were assessed in vitro. Results: TEM, FTIR, and UV–Vis–NIR studies confirmed side-wall functionalization of SWCNT with COOH-groups, PTX, POSS, and antibodies. Increased cell death was observed with PTX–POSS–SWCNT, PTX–POSS–Ab–SWCNT, and free PTX compared to functionalized-SWCNT (f-SWCNT, POSS–SWCNT, and cell-only controls at 48 and 72 h time intervals in both cell lines. At all time intervals, there was no significant cell death in the POSS–SWCNT samples compared to cell-only controls. Conclusion: The PTX-based nanocomposites were shown to be as cytotoxic as free PTX. This important finding indicates successful release of PTX from the nanocomposites and further reiterates the potential of SWCNTs to deliver drugs directly to targeted cells and tissues.

  4. Effectiveness of ACT-Based Parenting Training to Mothers on the Depression of Children with Cleft Lip and Palate: A Single Subject Study

    Directory of Open Access Journals (Sweden)

    محمد صالح فقیهی

    2017-06-01

    Full Text Available The purpose of this study was to determine the effectiveness of Parenting Training based on Acceptance and Commitment Therapy (ACT to mothers on the depression of children with cleft lip and palate. The research method was based on a single case and individual intervention study. The sample was constituted of 65 Isfahanian children with cleft lip and palate. Parenting skills based on ACT were taught to five mothers of children with cleft lip and palate who achieved the minimum score in screening. After three baseline sessions for each child, ACT parenting skills were taught to their mothers in 8 individual sessions companied with testing the child’s depression in every session. Three follow-up sessions after 15 days, 1 month and 3 months were set to evaluate children’s depression. The Kovacs Children’s Depression Inventory (CDI was used to test the children’s depression. The results were analyzed with visual analysis and descriptive statistics. This particular intervention was effective on depression. Based on the results of the present study, it can be concluded that ACT parenting training to mothers of children with cleft lips and palates was effective on reducing depression and that an on-time intervention can improve these children's depression.

  5. Receptor-targeted lentiviral vectors are exceptionally sensitive toward the biophysical properties of the displayed single-chain Fv.

    Science.gov (United States)

    Friedel, Thorsten; Hanisch, Lydia J; Muth, Anke; Honegger, Annemarie; Abken, Hinrich; Plückthun, Andreas; Buchholz, Christian J; Schneider, Irene C

    2015-04-01

    An increasing number of applications require the expression of single-chain variable fragments (scFv) fusion proteins in mammalian cells at the cell surface membrane. Here we assessed the CD30-specific scFv HRS3, which is used in immunotherapy, for its ability to retarget lentiviral vectors (LVs) to CD30 and to mediate selective gene transfer into CD30-positive cells. Fused to the C-terminus of the type-II transmembrane protein hemagglutinin (H) of measles virus and expressed in LV packaging cells, gene transfer mediated by the released LV particles was inefficient. A series of point mutations in the scFv framework regions addressing its biophysical properties, which substantially improved production and increased the melting temperature without impairing its kinetic binding behavior to CD30, also improved the performance of LV particles. Gene transfer into CD30-positive cells increased ∼100-fold due to improved transport of the H-scFv protein to the plasma membrane. Concomitantly, LV particle aggregation and syncytia formation in packaging cells were substantially reduced. The data suggest that syncytia formation can be triggered by trans-cellular dimerization of H-scFv proteins displayed on adjacent cells. Taken together, we show that the biophysical properties of the targeting ligand have a decisive role for the gene transfer efficiency of receptor-targeted LVs. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  6. Design and Generation of Humanized Single-chain Fv Derived from Mouse Hybridoma for Potential Targeting Application.

    Science.gov (United States)

    Khantasup, Kannika; Chantima, Warangkana; Sangma, Chak; Poomputsa, Kanokwan; Dharakul, Tararaj

    2015-12-01

    Single-chain variable antibody fragments (scFvs) are attractive candidates for targeted immunotherapy in several human diseases. In this study, a concise humanization strategy combined with an optimized production method for humanizing scFvs was successfully employed. Two antibody clones, one directed against the hemagglutinin of H5N1 influenza virus, the other against EpCAM, a cancer biomarker, were used to demonstrate the validity of the method. Heavy chain (VH) and light chain (VL) variable regions of immunoglobulin genes from mouse hybridoma cells were sequenced and subjected to the construction of mouse scFv 3-D structure. Based on in silico modeling, the humanized version of the scFv was designed via complementarity-determining region (CDR) grafting with the retention of mouse framework region (FR) residues identified by primary sequence analysis. Root-mean-square deviation (RMSD) value between mouse and humanized scFv structures was calculated to evaluate the preservation of CDR conformation. Mouse and humanized scFv genes were then constructed and expressed in Escherichia coli. Using this method, we successfully generated humanized scFvs that retained the targeting activity of their respective mouse scFv counterparts. In addition, the humanized scFvs were engineered with a C-terminal cysteine residue (hscFv-C) for site-directed conjugation for use in future targeting applications. The hscFv-C expression was extensively optimized to improve protein production yield. The protocol yielded a 20-fold increase in production of hscFv-Cs in E. coli periplasm. The strategy described in this study may be applicable in the humanization of other antibodies derived from mouse hybridoma.

  7. EDUCATIONAL TACTICS OF MOTHERS AND THEIR RELATION TO THE PSYCHOLOGICAL CHARACTERISTICS OF ADOLESCENTS IN TWO-PARENT AND SINGLE-PARENT FAMILIES

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    Zuev Konstantin Borisovich

    2013-03-01

    Full Text Available The paper presents the results of a study of the psychological characteristics of boys and girls from complete and incomplete families. In addition to the type of family, the authors consider the educational tactics of mothers. The combined effects of the type of family and educational tactics on psychological characteristics of the adolescent were investigated. Adolescence was chosen as a period, when on the one hand, a relatively stable personality structure is developed, and on the other hand, the importance of the immediate social environment is extremely high. For our study we selected the psychological characteristics, to the utmost revealing the reaction of children to their parents' divorce: the level of subjective control (degree of responsibility for their own lives, and the sovereignty of the psychological space (clearnesse of psychological boundaries. It is shown that the largest influence on the psychological characteristics of adolescents, regardless of the type of family, is rendered by hostility in maternal education. The dependence of psychological characteristics of maternal directiveness occurs only at high values ​​of such educational tactics that highlights the consistency of the obtained results with the classical

  8. Analysis of decisional conflict among parents who consent to hypospadias repair: single institution prospective study of 100 couples.

    Science.gov (United States)

    Lorenzo, Armando J; Braga, Luis H P; Zlateska, Bozana; Leslie, Bruno; Farhat, Walid A; Bägli, Darius J; Pippi Salle, Joao L

    2012-08-01

    Although obtaining informed consent for distal hypospadias repair is common practice, little is known about the uncertainty or conflict between consenting parents faced with this decision. We systematically evaluated decisional conflict between parents who elected to have their child undergo hypospadias surgery. A total of 100 couples who were counseled about treatment options agreed to participate. Using a validated questionnaire, the Decisional Conflict Scale, we prospectively collected data on decisional conflict demographics, preference for circumcision, education level and prior knowledge about hypospadias. All parents elected surgical repair. Evidence of decisional conflict was encountered in 28% of participants (score less than 25 in 72%, 25 to 37.5 in 23.5%, greater than 37.5 in 4.5%). No statistically significant differences among parents were noted for total score (mean ± SD 16.1 ± 12 in mothers and 18.3 ± 12.6 in fathers) or subscales, except the informed subscale (mean ± SD 16.7 ± 14.3 in mothers and 21.1 ± 16.6 in fathers). Parental self-report of prior knowledge about hypospadias and preference for neonatal circumcision correlated with lower Decisional Conflict Scale scores (p = 0.02 and p conflict in couples agreeing to proceed with hypospadias repair, with no evidence of significant discrepancy between them. The novel description of factors related to decreased decisional conflict might help focus efforts aimed at minimizing difficulties encountered during the decision making process. Copyright © 2012 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

  9. Investigating the effect of home care program on self-efficacy of femaleheaded single-parent households supported by Isfahan Welfare Organization (Behzisti in year 1394

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    Parvin Moeini

    2016-11-01

    Full Text Available Introduction: Female-headed households are among the most vulnerable groups of society and there are potential of many critical jeopardies for these families. Therefore, this study was focused to evaluate the effect of home care program on self-efficacy of female-headed single parent households supported by Isfahan Behzisti-Welfare Organization in year 2015. Methodology: This study was a quasi-experimental design research with random selection of 36 female headed single parent. Prior to the nursing intervention, demographic and life style information of these families were evaluated using 70 questions in a questionnaires. In addition, a pre-test using a standard self-efficacy questionnaires with 17 questions were used to assess the level of self-efficacy of these families. Following the experiment, these families responded to the self-efficacy questionnaires. For descriptive statistics analysis, frequency, average, and standard deviation were evaluated. Furthermore, paired-sample t-test, Pearson's chi-squared test, Mann–Whitney test, Spearman's rank-order correlation, and Kruskal–Wallis one-way analysis of variance were analyzed using SPSS v.18 software for statistical inference analysis. Results: Our analysis revealed that there was a significant difference (p<0.05 between the score average of pre- and post- home care program. It supported our hypothesis that the supportive home care program had significant effect on self-efficacy of femaleheaded single parent households. Discussion and Conclusion: Following the effect of the supportive nursing intervention on self-efficacy of female-headed households, it should be mentioned that this supportive program can be the best strategic plan to increase the family’s health and preventing health issues. Therefore, given the multiple roles of public health nurse, supportive home care program is critical and should be emphasized.

  10. Decisional regret after distal hypospadias repair: single institution prospective analysis of factors associated with subsequent parental remorse or distress.

    Science.gov (United States)

    Lorenzo, Armando J; Pippi Salle, João L; Zlateska, Bozana; Koyle, Martin A; Bägli, Darius J; Braga, Luis H P

    2014-05-01

    Hypospadias repair is a commonly performed procedure. Little is known about decisional regret in parents who agree to proceed with this surgical reconstruction. We present data on this previously underexplored issue. We performed followup analysis of 100 couples prospectively evaluated after counseling for surgical correction of distal hypospadias in their son with assessment of complications and decisional regret 1 year after surgery. Findings were contrasted with baseline demographics, hypospadias knowledge and decisional conflict at the time of counseling. Decisional regret was found in 116 parents, including mild regret in 41.4% and moderate to severe regret in 8.6%. There was no statistically significant difference in paired regret analysis between mothers and fathers. Complications were strongly associated with decisional regret (p <0.001). On regression analysis postoperative complications (OR 14.7, 95% CI 1.6-131.6), parental desire to avoid circumcision (OR 7.4, 95% CI 1.1-49.4) and initial decisional conflict level (OR 1.06, 95% CI 1.02-1.09) were statistically significant predictors of moderate to strong decisional regret. These findings remained robust after imputation strategies to address missing data. The impact of decisional conflict and preference for circumcision were significant even after excluding families who experienced complications. To our knowledge this is the first study demonstrating parental decisional regret after providing consent for surgical correction of distal hypospadias in their son. Based on the described risk factors efforts aimed at minimizing complications and counseling about foreskin preservation techniques may be prudent to ameliorate decisional regret. The novel association between decisional conflict and regret suggests that conflict assessment during counseling may help screen families at risk for postoperative regret. Copyright © 2014 American Urological Association Education and Research, Inc. Published by Elsevier

  11. The biological effect of large single doses: a possible role for non-targeted effects in cell inactivation.

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    Marlon R Veldwijk

    Full Text Available BACKGROUND AND PURPOSE: Novel radiotherapy techniques increasingly use very large dose fractions. It has been argued that the biological effect of large dose fractions may differ from that of conventional fraction sizes. The purpose was to study the biological effect of large single doses. MATERIAL AND METHODS: Clonogenic cell survival of MCF7 and MDA-MB-231 cells was determined after direct X-ray irradiation, irradiation of feeder cells, or transfer of conditioned medium (CM. Cell-cycle distributions and the apoptotic sub-G1 fraction were measured by flow cytometry. Cytokines in CM were quantified by a cytokine antibody array. γH2AX foci were detected by immunofluorescence microscopy. RESULTS: The surviving fraction of MCF7 cells irradiated in vitro with 12 Gy showed an 8.5-fold decrease (95% c.i.: 4.4-16.3; P<0.0001 when the density of irradiated cells was increased from 10 to 50×10(3 cells per flask. Part of this effect was due to a dose-dependent transferrable factor as shown in CM experiments in the dose range 5-15 Gy. While no effect on apoptosis and cell cycle distribution was observed, and no differentially expressed cytokine could be identified, the transferable factor induced prolonged expression of γH2AX DNA repair foci at 1-12 h. CONCLUSIONS: A dose-dependent non-targeted effect on clonogenic cell survival was found in the dose range 5-15 Gy. The dependence of SF on cell numbers at high doses would represent a "cohort effect" in vivo. These results support the hypothesis that non-targeted effects may contribute to the efficacy of very large dose fractions in radiotherapy.

  12. A cluster randomised controlled trial of a telephone-based intervention targeting the home food environment of preschoolers (The Healthy Habits Trial): the effect on parent fruit and vegetable consumption.

    Science.gov (United States)

    Wyse, Rebecca; Campbell, Karen J; Brennan, Leah; Wolfenden, Luke

    2014-12-24

    The home food environment is an important setting for the development of dietary patterns in childhood. Interventions that support parents to modify the home food environment for their children, however, may also improve parent diet. The purpose of this study was to assess the impact of a telephone-based intervention targeting the home food environment of preschool children on the fruit and vegetable consumption of parents. In 2010, 394 parents of 3-5 year-old children from 30 preschools in the Hunter region of Australia were recruited to this cluster randomised controlled trial and were randomly assigned to an intervention or control group. Intervention group parents received four weekly 30-minute telephone calls and written resources. The scripted calls focused on; fruit and vegetable availability and accessibility, parental role-modelling, and supportive home food routines. Two items from the Australian National Nutrition Survey were used to assess the average number of serves of fruit and vegetables consumed each day by parents at baseline, and 2-, 6-, 12-, and 18-months later, using generalised estimating equations (adjusted for baseline values and clustering by preschool) and an intention-to-treat-approach. At each follow-up, vegetable consumption among intervention parents significantly exceeded that of controls. At 2-months the difference was 0.71 serves (95% CI: 0.58-0.85, p food environment can increase parents' fruit and vegetable consumption. (ANZCTR12609000820202).

  13. Activated platelets in carotid artery thrombosis in mice can be selectively targeted with a radiolabeled single-chain antibody.

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    Timo Heidt

    Full Text Available BACKGROUND: Activated platelets can be found on the surface of inflamed, rupture-prone and ruptured plaques as well as in intravascular thrombosis. They are key players in thrombosis and atherosclerosis. In this study we describe the construction of a radiolabeled single-chain antibody targeting the LIBS-epitope of activated platelets to selectively depict platelet activation and wall-adherent non-occlusive thrombosis in a mouse model with nuclear imaging using in vitro and ex vivo autoradiography as well as small animal SPECT-CT for in vivo analysis. METHODOLOGY/PRINCIPAL FINDINGS: LIBS as well as an unspecific control single-chain antibody were labeled with (111Indium ((111In via bifunctional DTPA ( = (111In-LIBS/(111In-control. Autoradiography after incubation with (111In-LIBS on activated platelets in vitro (mean 3866 ± 28 DLU/mm(2, 4010 ± 630 DLU/mm(2 and 4520 ± 293 DLU/mm(2 produced a significantly higher ligand uptake compared to (111In-control (2101 ± 76 DLU/mm(2, 1181 ± 96 DLU/mm(2 and 1866 ± 246 DLU/mm(2 indicating a specific binding to activated platelets; P<0.05. Applying these findings to an ex vivo mouse model of carotid artery thrombosis revealed a significant increase in ligand uptake after injection of (111In-LIBS in the presence of small thrombi compared to the non-injured side, as confirmed by histology (49630 ± 10650 DLU/mm(2 vs. 17390 ± 7470 DLU/mm(2; P<0.05. These findings could also be reproduced in vivo. SPECT-CT analysis of the injured carotid artery with (111In-LIBS resulted in a significant increase of the target-to-background ratio compared to (111In-control (1.99 ± 0.36 vs. 1.1 ± 0.24; P < 0.01. CONCLUSIONS/SIGNIFICANCE: Nuclear imaging with (111In-LIBS allows the detection of platelet activation in vitro and ex vivo with high sensitivity. Using SPECT-CT, wall-adherent activated platelets in carotid arteries could be depicted in vivo. These results encourage further studies elucidating the role of

  14. Targeted Integration of Single-Copy Transgenes in Drosophila melanogaster Tissue-Culture Cells Using Recombination-Mediated Cassette Exchange.

    Science.gov (United States)

    Manivannan, Sathiya N; Jacobsen, Thomas L; Lyon, Peter; Selvaraj, Bhavani; Halpin, Peter; Simcox, Amanda

    2015-12-01

    Transfection of transgenes into Drosophila cultured cells is a standard approach for studying gene function. However, the number of transgenes present in the cell following transient transfection or stable random integration varies, and the resulting differences in expression level affect interpretation. Here we developed a system for Drosophila cell lines that allows selection of cells with a single-copy transgene inserted at a specific genomic site using recombination-mediated cassette exchange (RMCE). We used the φC31 integrase and its target sites attP and attB for RMCE. Cell lines with an attP-flanked genomic cassette were transfected with donor plasmids containing a transgene of interest (UAS-x), a dihydrofolate reductase (UAS-DHFR) gene flanked by attB sequences, and a thymidine kinase (UAS-TK) gene in the plasmid backbone outside the attB sequences. In cells undergoing RMCE, UAS-x and UAS-DHFR were exchanged for the attP-flanked genomic cassette, and UAS-TK was excluded. These cells were selected using methotrexate, which requires DHFR expression, and ganciclovir, which causes death in cells expressing TK. Pure populations of cells with one copy of a stably integrated transgene were efficiently selected by cloning or mass culture in ∼6 weeks. Our results show that RMCE avoids the problems associated with current methods, where transgene number is not controlled, and facilitates the rapid generation of Drosophila cell lines in which expression from a single transgene can be studied. Copyright © 2015 by the Genetics Society of America.

  15. The single cyclic nucleotide-specific phosphodiesterase of the intestinal parasite Giardia lamblia represents a potential drug target.

    Science.gov (United States)

    Kunz, Stefan; Balmer, Vreni; Sterk, Geert Jan; Pollastri, Michael P; Leurs, Rob; Müller, Norbert; Hemphill, Andrew; Spycher, Cornelia

    2017-09-01

    Giardiasis is an intestinal infection correlated with poverty and poor drinking water quality, and treatment options are limited. According to the Center for Disease Control and Prevention, Giardia infections afflict nearly 33% of people in developing countries, and 2% of the adult population in the developed world. This study describes the single cyclic nucleotide-specific phosphodiesterase (PDE) of G. lamblia and assesses PDE inhibitors as a new generation of anti-giardial drugs. An extensive search of the Giardia genome database identified a single gene coding for a class I PDE, GlPDE. The predicted protein sequence was analyzed in-silico to characterize its domain structure and catalytic domain. Enzymatic activity of GlPDE was established by complementation of a PDE-deficient Saccharomyces cerevisiae strain, and enzyme kinetics were characterized in soluble yeast lysates. The potency of known PDE inhibitors was tested against the activity of recombinant GlPDE expressed in yeast and against proliferating Giardia trophozoites. Finally, the localization of epitope-tagged and ectopically expressed GlPDE in Giardia cells was investigated. Giardia encodes a class I PDE. Catalytically important residues are fully conserved between GlPDE and human PDEs, but sequence differences between their catalytic domains suggest that designing Giardia-specific inhibitors is feasible. Recombinant GlPDE hydrolyzes cAMP with a Km of 408 μM, and cGMP is not accepted as a substrate. A number of drugs exhibit a high degree of correlation between their potency against the recombinant enzyme and their inhibition of trophozoite proliferation in culture. Epitope-tagged GlPDE localizes as dots in a pattern reminiscent of mitosomes and to the perinuclear region in Giardia. Our data strongly suggest that inhibition of G. lamblia PDE activity leads to a profound inhibition of parasite proliferation and that GlPDE is a promising target for developing novel anti-giardial drugs.

  16. Development and Characterization of a Camelid Single Domain Antibody-Urease Conjugate That Targets Vascular Endothelial Growth Factor Receptor 2.

    Science.gov (United States)

    Tian, Baomin; Wong, Wah Yau; Uger, Marni D; Wisniewski, Pawel; Chao, Heman

    2017-01-01

    Angiogenesis is the process of new blood vessel formation and is essential for a tumor to grow beyond a certain size. Tumors secrete the pro-angiogenic factor vascular endothelial growth factor, which acts upon local endothelial cells by binding to vascular endothelial growth factor receptors (VEGFRs). In this study, we describe the development and characterization of V21-DOS47, an immunoconjugate that targets VEGFR2. V21-DOS47 is composed of a camelid single domain anti-VEGFR2 antibody (V21) and the enzyme urease. The conjugate specifically binds to VEGFR2 and urease converts endogenous urea into ammonia, which is toxic to tumor cells. Previously, we developed a similar antibody-urease conjugate, L-DOS47, which is currently in clinical trials for non-small cell lung cancer. Although V21-DOS47 was designed from parameters learned from the generation of L-DOS47, additional optimization was required to produce V21-DOS47. In this study, we describe the expression and purification of two versions of the V21 antibody: V21H1 and V21H4. Each was conjugated to urease using a different chemical cross-linker. The conjugates were characterized by a panel of analytical techniques, including SDS-PAGE, size exclusion chromatography, Western blotting, and LC-MS E peptide mapping. Binding characteristics were determined by ELISA and flow cytometry assays. To improve the stability of the conjugates at physiologic pH, the pIs of the V21 antibodies were adjusted by adding several amino acid residues to the C-terminus. For V21H4, a terminal cysteine was also added for use in the conjugation chemistry. The modified V21 antibodies were expressed in the E. coli BL21 (DE3) pT7 system. V21H1 was conjugated to urease using the heterobifunctional cross-linker succinimidyl-[( N -maleimidopropionamido)-diethyleneglycol] ester (SM(PEG) 2 ), which targets lysine resides in the antibody. V21H4 was conjugated to urease using the homobifunctional cross-linker, 1,8-bis(maleimido)diethylene glycol

  17. Development and Characterization of a Camelid Single Domain Antibody–Urease Conjugate That Targets Vascular Endothelial Growth Factor Receptor 2

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    Baomin Tian

    2017-08-01

    Full Text Available Angiogenesis is the process of new blood vessel formation and is essential for a tumor to grow beyond a certain size. Tumors secrete the pro-angiogenic factor vascular endothelial growth factor, which acts upon local endothelial cells by binding to vascular endothelial growth factor receptors (VEGFRs. In this study, we describe the development and characterization of V21-DOS47, an immunoconjugate that targets VEGFR2. V21-DOS47 is composed of a camelid single domain anti-VEGFR2 antibody (V21 and the enzyme urease. The conjugate specifically binds to VEGFR2 and urease converts endogenous urea into ammonia, which is toxic to tumor cells. Previously, we developed a similar antibody–urease conjugate, L-DOS47, which is currently in clinical trials for non-small cell lung cancer. Although V21-DOS47 was designed from parameters learned from the generation of L-DOS47, additional optimization was required to produce V21-DOS47. In this study, we describe the expression and purification of two versions of the V21 antibody: V21H1 and V21H4. Each was conjugated to urease using a different chemical cross-linker. The conjugates were characterized by a panel of analytical techniques, including SDS-PAGE, size exclusion chromatography, Western blotting, and LC-MSE peptide mapping. Binding characteristics were determined by ELISA and flow cytometry assays. To improve the stability of the conjugates at physiologic pH, the pIs of the V21 antibodies were adjusted by adding several amino acid residues to the C-terminus. For V21H4, a terminal cysteine was also added for use in the conjugation chemistry. The modified V21 antibodies were expressed in the E. coli BL21 (DE3 pT7 system. V21H1 was conjugated to urease using the heterobifunctional cross-linker succinimidyl-[(N-maleimidopropionamido-diethyleneglycol] ester (SM(PEG2, which targets lysine resides in the antibody. V21H4 was conjugated to urease using the homobifunctional cross-linker, 1,8-bis

  18. Differential effects of baseline drinking status: effects of an alcohol prevention program targeting students and/or parents (PAS) among weekly drinking students.

    Science.gov (United States)

    Koning, Ina M; Lugtig, Peter; Vollebergh, Wilma A M

    2014-04-01

    The effects of an intervention designed to prevent onset of weekly drinking in non drinking students (PAS) were investigated in the group of students that was already drinking at baseline. A cluster randomized trial was used including 3,490 Dutch early adolescents (M age = 12.66, SD = 0.49) randomized over four conditions; 1) parent intervention, 2) student intervention, 3) combined intervention and 4) control group. Outcome measures were amount and growth of weekly alcohol drinking measured 10, 22 and 34 months after baseline. The combined intervention significantly curbed the growth of drinking among both non-drinkers (the target group of the intervention) and drinkers at baseline. Overall, less strong increases of drinking over time are found among non-drinkers compared to drinkers at baseline. Thus, the combined PAS intervention is also effective in curbing adolescents' drinking behaviour in those who already were drinking at baseline. Broad implementation of the combined parent-student intervention is recommended. Copyright © 2014 Elsevier Inc. All rights reserved.

  19. Strand Invasion Based Amplification (SIBA®): a novel isothermal DNA amplification technology demonstrating high specificity and sensitivity for a single molecule of target analyte.

    Science.gov (United States)

    Hoser, Mark J; Mansukoski, Hannu K; Morrical, Scott W; Eboigbodin, Kevin E

    2014-01-01

    Isothermal nucleic acid amplification technologies offer significant advantages over polymerase chain reaction (PCR) in that they do not require thermal cycling or sophisticated laboratory equipment. However, non-target-dependent amplification has limited the sensitivity of isothermal technologies and complex probes are usually required to distinguish between non-specific and target-dependent amplification. Here, we report a novel isothermal nucleic acid amplification technology, Strand Invasion Based Amplification (SIBA). SIBA technology is resistant to non-specific amplification, is able to detect a single molecule of target analyte, and does not require target-specific probes. The technology relies on the recombinase-dependent insertion of an invasion oligonucleotide (IO) into the double-stranded target nucleic acid. The duplex regions peripheral to the IO insertion site dissociate, thereby enabling target-specific primers to bind. A polymerase then extends the primers onto the target nucleic acid leading to exponential amplification of the target. The primers are not substrates for the recombinase and are, therefore unable to extend the target template in the absence of the IO. The inclusion of 2'-O-methyl RNA to the IO ensures that it is not extendible and that it does not take part in the extension of the target template. These characteristics ensure that the technology is resistant to non-specific amplification since primer dimers or mis-priming are unable to exponentially amplify. Consequently, SIBA is highly specific and able to distinguish closely-related species with single molecule sensitivity in the absence of complex probes or sophisticated laboratory equipment. Here, we describe this technology in detail and demonstrate its use for the detection of Salmonella.

  20. Strand Invasion Based Amplification (SIBA®: a novel isothermal DNA amplification technology demonstrating high specificity and sensitivity for a single molecule of target analyte.

    Directory of Open Access Journals (Sweden)

    Mark J Hoser

    Full Text Available Isothermal nucleic acid amplification technologies offer significant advantages over polymerase chain reaction (PCR in that they do not require thermal cycling or sophisticated laboratory equipment. However, non-target-dependent amplification has limited the sensitivity of isothermal technologies and complex probes are usually required to distinguish between non-specific and target-dependent amplification. Here, we report a novel isothermal nucleic acid amplification technology, Strand Invasion Based Amplification (SIBA. SIBA technology is resistant to non-specific amplification, is able to detect a single molecule of target analyte, and does not require target-specific probes. The technology relies on the recombinase-dependent insertion of an invasion oligonucleotide (IO into the double-stranded target nucleic acid. The duplex regions peripheral to the IO insertion site dissociate, thereby enabling target-specific primers to bind. A polymerase then extends the primers onto the target nucleic acid leading to exponential amplification of the target. The primers are not substrates for the recombinase and are, therefore unable to extend the target template in the absence of the IO. The inclusion of 2'-O-methyl RNA to the IO ensures that it is not extendible and that it does not take part in the extension of the target template. These characteristics ensure that the technology is resistant to non-specific amplification since primer dimers or mis-priming are unable to exponentially amplify. Consequently, SIBA is highly specific and able to distinguish closely-related species with single molecule sensitivity in the absence of complex probes or sophisticated laboratory equipment. Here, we describe this technology in detail and demonstrate its use for the detection of Salmonella.

  1. Volatiles emitted from single flower buds of the lilium longiflorum × L. callosum interspecific hybrid and its parents

    Science.gov (United States)

    This research was initiated to analyze the volatiles emitted from a single flower bud of Lilium longiflorum Thunb., L. callosum Sieb. et Zucc., and interspecific hybrids of L. longiflorum × L. callosum (L. longi × L. cal IH). Volatiles, collected automatically every 6-hour intervals at 20oC before ...

  2. [Parents support for the ban on television food advertising to children is particularly high in France, especially compared to the USA. This result should influence political decision-making to restrict food marketing targeting young people].

    Science.gov (United States)

    Escalon, Hélène; Cogordan, Chloé; Arwidson, Pierre

    2016-01-01

    Massive exposure of children to low-nutrient food advertising combined with the increasing prevalence of obesity have led to growing support for statutory regulations concerning food marketing targeting children. Food and advertising industries lobbies have nevertheless managed to stop such measures in many countries. In this context, civil society support for statutory regulation, especially by parents, is essential. The objective of this study was to describe and analyse factors associated with parents' opinion on the impact and possible banning of food TV advertisements targeting children. An online survey of 2,387 parents of children aged 3 to 17 was conducted in 2013. Associations between parents'opinion on food advertising and their socio-demographic characteristics were analysed by multivariate logistic regressions. The influence of food advertising on children' preferences was perceived by 64.7% of parents, 68.8% of parents were at least occasionally asked by their children to purchase food or beverages seen on television, 43.5% reported that their children influenced their food purchases and 73.7% supported a statutory regulation that would ban advertisements for excessively fatty, salty and sugary beverages and foods during television programmes for children or teenagers. This view was positively associated with high socio-economic status and a high perceived impact of advertising on children's food preferences. Parents support for the ban on television food advertising to children is particularly high in France, especially compared to the USA. This result should influence political decision-making to restrict food marketing targeting young people.

  3. Why target early adolescents and parents in alcohol prevention? The mediating effects of self-control, rules and attitudes about alcohol use

    NARCIS (Netherlands)

    Koning, I.M.; Eijnden, R.J.J.M. van den; Engels, R.C.M.E.; Verdurmen, J.E.E.; Vollebergh, W.A.M.

    2011-01-01

    Aims To examine the effects of a parent and student intervention offered separately and simultaneously (PAS) on onset of weekly drinking via its putative mediators. Design A randomized trial with four conditions; (1) parent intervention, (2) student intervention, (3) combined parent-student

  4. Siblings and Parents in One-Parent Families.

    Science.gov (United States)

    Shapiro, Edna K.; Wallace, Doris B.

    1987-01-01

    Examination of six one- and two-parent families reveals the following: (1) members of the same family have different perceptions of family experiences, including divorce and its impact on family relationships; (2) single parents seem to become closer to their children than do married parents; and (3) a one-parent two-sibling family differs from a…

  5. Eradication of large solid tumors by gene therapy with a T cell receptor targeting a single cancer-specific point mutation

    Science.gov (United States)

    Leisegang, Matthias; Engels, Boris; Schreiber, Karin; Yew, Poh Yin; Kiyotani, Kazuma; Idel, Christian; Arina, Ainhoa; Duraiswamy, Jaikumar; Weichselbaum, Ralph R.; Uckert, Wolfgang; Nakamura, Yusuke; Schreiber, Hans

    2015-01-01

    Purpose Cancers usually contain multiple unique tumor-specific antigens produced by single amino acid substitutions (AAS) and encoded by somatic non-synonymous single nucleotide substitutions. We determined whether adoptively transferred T cells can reject large, well-established solid tumors when engineered to express a single type of T cell receptor (TCR) that is specific for a single AAS. Experimental Design By exome and RNA sequencing of an UV-induced tumor, we identified an AAS in p68 (mp68), a co-activator of p53. This AAS seemed to be an ideal tumor-specific neoepitope because it is encoded by a trunk mutation in the primary autochthonous cancer and binds with highest affinity to the MHC. A high-avidity mp68-specific TCR was used to genetically engineer T cells as well as to generate TCR-transgenic mice for adoptive therapy. Results When the neoepitope was expressed at high levels and by all cancer cells, their direct recognition sufficed to destroy intra-tumor vessels and eradicate large, long-established solid tumors. When the neoepitope was targeted as autochthonous antigen, T cells caused cancer regression followed by escape of antigen-negative variants. Escape could be thwarted by expressing the antigen at increased levels in all cancer cells or by combining T cell therapy with local irradiation. Therapeutic efficacies of TCR-transduced and TCR-transgenic T cells were similar. Conclusions Gene therapy with a single TCR targeting a single AAS can eradicate large established cancer but a uniform expression and/or sufficient levels of the targeted neoepitope or additional therapy are required to overcome tumor escape. PMID:26667491

  6. The new target chamber at LIPSION: The new translation stage and goniometer and the new irradiation platform for single cell experiments

    International Nuclear Information System (INIS)

    Nilsson, Charlotta; Petriconi, Silvio; Reinert, Tilo; Butz, Tilman

    2007-01-01

    A new target chamber as well as a new 7-axes translation stage with goniometer will shortly be implemented at the LIPSION nanoprobe in Leipzig. This new stage should enable linear motion as well as rotary motion with high precision, positioning accuracy and repeatability. These different features have been investigated online as well as offline, with encouraging results. Along with the new equipment, new software is also being developed, to provide stage control running over network. These software developments, including a graphical user interface, will also be described. As part of the new target chamber, a new external beam facility and irradiation platform for single ion experiments on single living cells is being assembled. A detailed description of the new cell irradiation platform, including beam extraction, new cell dishes, and cell recognition aspects will be given. It will be shown that the possibility of offline cell recognition, possibly using a differential interference contrast microscope, is an option

  7. Meaurement of the target single-spin asymmetry in quasi-elastic region from the reaction {sup 3}He{up_arrow}(e,e')

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Yawei [Rutgers

    2013-10-01

    A measurement of the inclusive target single-spin asymmetry has been performed using the quasi-elastic {sup 3}He{up_arrow}(e,e') reaction with a vertically polarized {sup 3}He target at Q{sup 2} values of 0.13, 0.46 and 0.97 GeV{sup 2}. This asymmetry vanishes under the one photon exchange assumption. But the interference between two-photon exchange and one-photon exchange gives rise to an imaginary amplitude, so that a non-zero A{sub y} is allowed. The experiment, conducted in Hall A of Jefferson Laboratory in 2009, used two independent spectrometers to simultaneously measure the target single-spin asymmetry. Using the effective polarization approximation, the neutron single-spin asymmetries were extracted from the measured {sup 3}He asymmetries. The measurement is to establish a non-vanishing A{sub y}. Non-zero asymmetries were observed at all Q{sup 2} points, and the overall precision is an order of magnitude improved over the existing proton data. The data provide new constraints on Generalized Parton Distribution (GPD) models and new information on the dynamics of the two-photon exchange process.

  8. Development of a method to extract and purify target compounds from medicinal plants in a single step: online hyphenation of expanded bed adsorption chromatography and countercurrent chromatography

    Science.gov (United States)

    Li, Yang; Wang, Nan; Zhang, Min; Ito, Yoichiro; Zhang, Hongyang; Wang, Yuerong; Guo, Xin; Hu, Ping

    2014-01-01

    Pure compounds extracted and purified from natural sources are crucial to lead discovery and drug screening. This study presents a novel two-dimensional hyphenation of expanded bed adsorption chromatography (EBAC) and high-speed countercurrent chromatography (HSCCC) for extraction and purification of target compounds from medicinal plants in a single step. The EBAC and HSCCC were hyphenated via a six-port injection valve as an interface. Fractionation of ingredients of Salvia miltiorrhiza and Rhizoma coptidis was performed on the hyphenated system to verify its efficacy. An amount each of 52.9 mg of salvianolic acid B and 2.1 mg of rosmarinic acid was obtained from Salvia miltiorrhiza by the two-dimensional system in a single step. The purities of the targets were over 96% of salvianolic acid B and 74% of rosmarinic acid. An amount each of 4.6 mg of coptisine and 4.1 mg of berberine was obtained from Rhizoma coptidis each with 98% and 82% purity, respectively. The processing time was nearly 50% that of the multi-step method. These results indicate that the present method is a rapid and green way to harvest targets from medicinal plants in a single step. PMID:24588208

  9. Compatibility of the repairable-conditionally repairable, multi-target and linear-quadratic models in converting hypofractionated radiation doses to single doses.

    Science.gov (United States)

    Iwata, Hiromitsu; Matsufuji, Naruhiro; Toshito, Toshiyuki; Akagi, Takashi; Otsuka, Shinya; Shibamoto, Yuta

    2013-03-01

    We investigated the applicability of the repairable-conditionally repairable (RCR) model and the multi-target (MT) model to dose conversion in high-dose-per-fraction radiotherapy in comparison with the linear-quadratic (LQ) model. Cell survival data of V79 and EMT6 single cells receiving single doses of 2-12 Gy or 2 or 3 fractions of 4 or 5 Gy each, and that of V79 spheroids receiving single doses of 5-26 Gy or 2-5 fractions of 5-12 Gy, were analyzed. Single and fractionated doses to actually reduce cell survival to the same level were determined by a colony assay. Single doses used in the experiments and surviving fractions at the doses were substituted into equations of the RCR, MT and LQ models in the calculation software Mathematica, and each parameter coefficient was computed. Thereafter, using the coefficients and the three models, equivalent single doses for the hypofractionated doses were calculated. They were then compared with actually-determined equivalent single doses for the hypofractionated doses. The equivalent single doses calculated using the RCR, MT and LQ models tended to be lower than the actually determined equivalent single doses. The LQ model seemed to fit relatively well at doses of 5 Gy or less. At 6 Gy or higher doses, the RCR and MT models seemed to be more reliable than the LQ model. In hypofractionated stereotactic radiotherapy, the LQ model should not be used, and conversion models incorporating the concept of the RCR or MT models, such as the generalized linear-quadratic models, appear to be more suitable.

  10. Evaluation of single and dual siRNAs targeting rabies virus glycoprotein and nucleoprotein genes for inhibition of virus multiplication in vitro.

    Science.gov (United States)

    Meshram, Chetan D; Singh, Niraj K; Sonwane, Arvind A; Pawar, Sachin S; Mishra, B P; Chaturvedi, V K; Saini, Mohini; Singh, R P; Gupta, Praveen K

    2013-11-01

    Small interfering RNAs (siRNAs) targeting rabies virus (RV) glycoprotein (G) and nucleoprotein (N) genes were evaluated as antiviral agents against rabies virus in vitro in BHK-21 cells. To select effective siRNAs targeting RV-G, a plasmid-based transient co-transfection approach was used. In this, siRNAs were expressed as short hairpin RNAs (shRNAs), and their ability to inhibit RV-G gene expression was evaluated in cells transfected with a plasmid expressing RV-G. The nine different siRNAs designed to target RV-G exhibited varying degrees of knockdown of RV-G gene expression. One siRNA (si-G7) with considerable effect in knockdown of RV-G expression also demonstrated significant inhibition of RV multiplication in BHK-21 cells after in vitro challenge with the RV Pasteur virus-11 (PV-11) strain. A decrease in the number of fluorescent foci in siRNA-treated cells and a reduction (86.8 %) in the release of RV into infected cell culture supernatant indicated the anti-rabies potential of siRNA. Similarly, treatment with one siRNA targeting RV-N resulted in a decrease in the number of fluorescent foci and a reduction (85.9 %) in the release of RV. As a dual gene silencing approach where siRNAs targeting RV-G and RV-N genes were expressed from single construct, the anti-rabies-virus effect was observed as an 87.4 % reduction in the release of RV. These results demonstrate that siRNAs targeting RV-G and N, both in single and dual form, have potential as antiviral agent against rabies.

  11. Defining the risk of toxicity in phase I oncology trials of novel molecularly targeted agents: A single-center experience

    NARCIS (Netherlands)

    Fehrmann, Rudolf

    2010-01-01

    Background: Phase I oncology trials are designed to define the maximum tolerated dose and toxicity of new drugs. This study aims to define the risk of toxicity in phase I trials of novel molecularly targeted agents (MTA), as this therapeutic approach is becoming increasingly relevant. Methods: We

  12. Defining the risk of toxicity in phase I oncology trials of novel molecularly targeted agents : a single centre experience

    NARCIS (Netherlands)

    Molife, L R; Alam, S; Olmos, D; Puglisi, M; Shah, K; Fehrmann, Rudolf; Trani, L; Tjokrowidjaja, A; de Bono, J S; Banerji, U; Kaye, S B

    BACKGROUND: This study defined the risk of serious toxicity in phase I trials of molecularly targeted agents (MTA). PATIENTS AND METHODS: A retrospective analysis of toxicity data from patients treated in phase I trials of MTAs was carried out to define the rate of treatment-related grade 3/4 toxic

  13. Social Skills Intervention Planning for Preschoolers: Using the SSiS-Rating Scales to Identify Target Behaviors Valued by Parents and Teachers

    Science.gov (United States)

    Frey, Jennifer R.; Elliott, Stephen N.; Kaiser, Ann P.

    2014-01-01

    Teachers' and parents' importance ratings of social behaviors for 95 preschoolers were examined using the "Social Skills Improvement System-Rating Scales" (Gresham & Elliott, 2008). Multivariate analyses were used to examine parents' and teachers' importance ratings at the item and subscale levels. Overall,…

  14. Adolescent Sexual Health Education: Parents Benefit Too!

    Science.gov (United States)

    Dinaj-Koci, Veronica; Deveaux, Lynette; Wang, Bo; Lunn, Sonya; Marshall, Sharon; Li, Xiaoming; Stanton, Bonita

    2015-01-01

    The inclusion of parents in adolescent-targeted interventions is intended to benefit the adolescent. Limited research has explored whether parents participating in these programs also benefit directly. We examined the impact of Caribbean Informed Parents and Children Together, the parenting portion of an adolescent-targeted HIV prevention…

  15. Neighborhood-targeted and case-triggered use of a single dose of oral cholera vaccine in an urban setting: Feasibility and vaccine coverage.

    Science.gov (United States)

    Parker, Lucy A; Rumunu, John; Jamet, Christine; Kenyi, Yona; Lino, Richard Laku; Wamala, Joseph F; Mpairwe, Allan M; Muller, Vincent; Llosa, Augusto E; Uzzeni, Florent; Luquero, Francisco J; Ciglenecki, Iza; Azman, Andrew S

    2017-06-01

    In June 2015, a cholera outbreak was declared in Juba, South Sudan. In addition to standard outbreak control measures, oral cholera vaccine (OCV) was proposed. As sufficient doses to cover the at-risk population were unavailable, a campaign using half the standard dosing regimen (one-dose) targeted high-risk neighborhoods and groups including neighbors of suspected cases. Here we report the operational details of this first public health use of a single-dose regimen of OCV and illustrate the feasibility of conducting highly targeted vaccination campaigns in an urban area. Neighborhoods of the city were prioritized for vaccination based on cumulative attack rates, active transmission and local knowledge of known cholera risk factors. OCV was offered to all persons older than 12 months at 20 fixed sites and to select groups, including neighbors of cholera cases after the main campaign ('case-triggered' interventions), through mobile teams. Vaccination coverage was estimated by multi-stage surveys using spatial sampling techniques. 162,377 individuals received a single-dose of OCV in the targeted neighborhoods. In these neighborhoods vaccine coverage was 68.8% (95% Confidence Interval (CI), 64.0-73.7) and was highest among children ages 5-14 years (90.0%, 95% CI 85.7-94.3), with adult men being less likely to be vaccinated than adult women (Relative Risk 0.81, 95% CI: 0.68-0.96). In the case-triggered interventions, each lasting 1-2 days, coverage varied (range: 30-87%) with an average of 51.0% (95% CI 41.7-60.3). Vaccine supply constraints and the complex realities where cholera outbreaks occur may warrant the use of flexible alternative vaccination strategies, including highly-targeted vaccination campaigns and single-dose regimens. We showed that such campaigns are feasible. Additional work is needed to understand how and when to use different strategies to best protect populations against epidemic cholera.

  16. CLoNe is a new method to target single progenitors and study their progeny in mouse and chick.

    Science.gov (United States)

    García-Moreno, Fernando; Vasistha, Navneet A; Begbie, Jo; Molnár, Zoltán

    2014-04-01

    Cell lineage analysis enables us to address pivotal questions relating to: the embryonic origin of cells and sibling cell relationships in the adult body; the contribution of progenitors activated after trauma or disease; and the comparison across species in evolutionary biology. To address such fundamental questions, several techniques for clonal labelling have been developed, each with its shortcomings. Here, we report a novel method, CLoNe that is designed to work in all vertebrate species and tissues. CLoNe uses a cocktail of labelling, targeting and transposition vectors that enables targeting of specific subpopulations of progenitor types with a combination of fluorophores resulting in multifluorescence that describes multiple clones per specimen. Furthermore, transposition into the genome ensures the longevity of cell labelling. We demonstrate the robustness of this technique in mouse and chick forebrain development, and show evidence that CLoNe will be broadly applicable to study clonal relationships in different tissues and species.

  17. An MRI-visible non-viral vector bearing GD2 single chain antibody for targeted gene delivery to human bone marrow mesenchymal stem cells.

    Directory of Open Access Journals (Sweden)

    Pengfei Pang

    Full Text Available The neural ganglioside GD2 has recently been reported to be a novel surface marker that is only expressed on human bone marrow mesenchymal stem cells within normal marrow. In this study, an MRI-visible, targeted, non-viral vector for effective gene delivery to human bone marrow mesenchymal stem cells was first synthesized by attaching a targeting ligand, the GD2 single chain antibody (scAbGD2, to the distal ends of PEG-g-PEI-SPION. The targeted vector was then used to condense plasmid DNA to form nanoparticles showing stable small size, low cytotoxicity, and good biocompatibility. Based on a reporter gene assay, the transfection efficiency of targeting complex reached the highest value at 59.6% ± 4.5% in human bone marrow mesenchymal stem cells, which was higher than those obtained using nontargeting complex and lipofectamine/pDNA (17.7% ± 2.9% and 34.9% ± 3.6%, respectively (P<0.01. Consequently, compared with the nontargeting group, more in vivo gene expression was observed in the fibrotic rat livers of the targeting group. Furthermore, the targeting capacity of scAbGD2-PEG-g-PEI-SPION was successfully verified in vitro by confocal laser scanning microscopy, Prussian blue staining, and magnetic resonance imaging. Our results indicate that scAbGD2-PEG-g-PEI-SPION is a promising MRI-visible non-viral vector for targeted gene delivery to human bone marrow mesenchymal stem cells.

  18. On-site detection of Phytophthora spp.—single-stranded target DNA as the limiting factor to improve on-chip hybridization

    International Nuclear Information System (INIS)

    Schwenkbier, Lydia; Pollok, Sibyll; Popp, Jürgen; Weber, Karina; König, Stephan; Wagner, Stefan; Werres, Sabine; Weber, Jörg; Hentschel, Martin

    2014-01-01

    We report on a lab-on-a-chip approach for on-site detection of Phytophthora species that allows visual signal readout. The results demonstrate the significance of single-stranded DNA (ssDNA) generation in terms of improving the intensity of the hybridization signal and to improve the reliability of the method. Conventional PCR with subsequent heat denaturation, sodium hydroxide-based denaturation, lambda exonuclease digestion and two asymmetric PCR methods were investigated for the species P. fragariae, P. kernoviae, and P. ramorum. The positioning of the capture probe within the amplified yeast GTP-binding protein (YPT1) target DNA was also of interest because it significantly influences the intensity of the signal. Statistical tests were used to validate the impact of the ssDNA generation methods and the capture-target probe position. The single-stranded target DNA generated by Linear-After-The-Exponential PCR (LATE-PCR) was found to produce signal intensities comparable to post-PCR exonuclease treatment. The LATE-PCR is the best method for the on-site detection of Phytophthora because the enzymatic digestion after PCR is more laborious and time-consuming. (author)

  19. Human Relations Activities for the Single Parent To Develop More Effective Parent/Child Relations. Bilingual Guide = Actividades sobre relaciones humanas para ayudar al padre-soltero o madre-soltera a desarrollar una relacion efectiva entre padre e hijo Guia bilingue.

    Science.gov (United States)

    Peterson, Susan

    Written in English and Spanish, this bilingual guide offers 10 activities which single parents can use to improve their relationships with their children. Objectives of activities include: (1) developing children's responsibility for work tasks in the home; (2) improving sibling relationships; (3) discussing emergencies with children; (4)…

  20. Parental Alienation Syndrome

    Directory of Open Access Journals (Sweden)

    Fuat Torun

    2011-09-01

    Full Text Available Children who have been programmed by one parent to be alienated from the other parent are commonly seen in the context of child-custody disputes. Its primary manifestation is the child’s campaign of denigration against a parent, a campaign that has no justification. It is said to result from a combination of a programming (brainwashing parent’s indoctrinations and the child’s own contributions to the vilification of the targeted parent. Many evaluators use the term parental alienation syndrome to refer to the disorder engendered in such children. However, there is significant controversy going on about the validity of parental alienation syndrome. The purpose of this article has been to describe and help to differentiate parental alienation syndrome and abuse for mental health professionals working in the field, and discuss the arguments about the validity of this syndrome.

  1. Parenting Multiples

    Science.gov (United States)

    ... Staying Safe Videos for Educators Search English Español Parenting Multiples KidsHealth / For Parents / Parenting Multiples What's in ... your ability to take care of your babies. Parenting Issues With Multiples It may be difficult to ...

  2. Asteroseismology of OB stars with hundreds of single snapshot spectra (and a few time-series of selected targets)

    Science.gov (United States)

    Simón-Díaz, S.

    2015-01-01

    Imagine we could do asteroseismology of large samples of OB-type stars by using just one spectrum per target. That would be great! But this is probably a crazy and stupid idea. Or maybe not. Maybe we have the possibility to open a new window to investigate stellar oscillations in massive stars that has been in front of us for many years, but has not attracted very much our attention: the characterization and understanding of the so-called macroturbulent broadening in OB-type stars.

  3. Immunotherapy (For Parents)

    Science.gov (United States)

    ... Staying Safe Videos for Educators Search English Español Immunotherapy KidsHealth / For Parents / Immunotherapy What's in this article? ... Types of Immunotherapy Side Effects Outlook Print About Immunotherapy Immunotherapy, also known as targeted therapy or biotherapy, ...

  4. Selection and Characterization of Single Chain Antibody Fragments Specific for Hsp90 as a Potential Cancer Targeting Molecule

    Directory of Open Access Journals (Sweden)

    Edyta Petters

    2015-08-01

    Full Text Available Heat shock proteins play an essential role in facilitating malignant transformation and they have been recognized as important factors in human cancers. One of the key elements of the molecular chaperones machinery is Hsp90 and it has recently become a target for anticancer therapeutic approaches. The potential and importance of Hsp90-directed agents becomes apparent when one realizes that disruption of Hsp90 function may influence over 200 oncogenic client proteins. Here, we described the selection and characterization of Hsp90-specific antibody fragments from commercially available Tomlinson I and J phage display libraries. The affinities of Hsp90-binding scFv variants were measured using SPR method. Then, based on the best clone selected, we performed the affinity maturation procedure and obtained valuable Hsp90-specific clones. The selected binders were expressed and applied for immunostaining, ELISA and SPR analysis using model cancer cell lines. All performed experiments confirmed the ability of selected antibodies to interact with the Hsp90. Therefore, the presented Hsp90-specific scFv, might be a starting point for the development of a novel antibody-based strategy targeting cancer.

  5. Molecular diagnostics of a single drug-resistant multiple myeloma case using targeted next-generation sequencing

    Directory of Open Access Journals (Sweden)

    Ikeda H

    2015-10-01

    Full Text Available Hiroshi Ikeda,1 Kazuya Ishiguro,1 Tetsuyuki Igarashi,1 Yuka Aoki,1 Toshiaki Hayashi,1 Tadao Ishida,1 Yasushi Sasaki,1,2 Takashi Tokino,2 Yasuhisa Shinomura1 1Department of Gastroenterology, Rheumatology and Clinical Immunology, 2Medical Genome Sciences, Research Institute for Frontier Medicine, Sapporo Medical University, Sapporo, Japan Abstract: A 69-year-old man was diagnosed with IgG λ-type multiple myeloma (MM, Stage II in October 2010. He was treated with one cycle of high-dose dexamethasone. After three cycles of bortezomib, the patient exhibited slow elevations in the free light-chain levels and developed a significant new increase of serum M protein. Bone marrow cytogenetic analysis revealed a complex karyotype characteristic of malignant plasma cells. To better understand the molecular pathogenesis of this patient, we sequenced for mutations in the entire coding regions of 409 cancer-related genes using a semiconductor-based sequencing platform. Sequencing analysis revealed eight nonsynonymous somatic mutations in addition to several copy number variants, including CCND1 and RB1. These alterations may play roles in the pathobiology of this disease. This targeted next-generation sequencing can allow for the prediction of drug resistance and facilitate improvements in the treatment of MM patients. Keywords: multiple myeloma, drug resistance, genome-wide sequencing, semiconductor sequencer, target therapy

  6. M13 phage-functionalized single-walled carbon nanotubes as nanoprobes for second near-infrared window fluorescence imaging of targeted tumors.

    Science.gov (United States)

    Yi, Hyunjung; Ghosh, Debadyuti; Ham, Moon-Ho; Qi, Jifa; Barone, Paul W; Strano, Michael S; Belcher, Angela M

    2012-03-14

    Second near-infrared (NIR) window light (950-1400 nm) is attractive for in vivo fluorescence imaging due to its deep penetration depth in tissues and low tissue autofluorescence. Here we show genetically engineered multifunctional M13 phage can assemble fluorescent single-walled carbon nanotubes (SWNTs) and ligands for targeted fluorescence imaging of tumors. M13-SWNT probe is detectable in deep tissues even at a low dosage of 2 μg/mL and up to 2.5 cm in tissue-like phantoms. Moreover, targeted probes show specific and up to 4-fold improved uptake in prostate specific membrane antigen positive prostate tumors compared to control nontargeted probes. This M13 phage-based second NIR window fluorescence imaging probe has great potential for specific detection and therapy monitoring of hard-to-detect areas. © 2012 American Chemical Society

  7. Potent and selective antisense oligonucleotides targeting single-nucleotide polymorphisms in the Huntington disease gene / allele-specific silencing of mutant huntingtin

    DEFF Research Database (Denmark)

    Carroll, Jeffrey B; Warby, Simon C; Southwell, Amber L

    2011-01-01

    Huntington disease (HD) is an autosomal dominant neurodegenerative disorder caused by CAG-expansion in the huntingtin gene (HTT) that results in a toxic gain of function in the mutant huntingtin protein (mHTT). Reducing the expression of mHTT is therefore an attractive therapy for HD. However, wild......-type HTT protein is essential for development and has critical roles in maintaining neuronal health. Therapies for HD that reduce wild-type HTT may therefore generate unintended negative consequences. We have identified single-nucleotide polymorphism (SNP) targets in the human HD population for the disease...

  8. Total cross-sections for single electron capture from H, He and H2 targets by impact of Be4+ and B5+ ions

    International Nuclear Information System (INIS)

    Busnengo, H.F.; Rivarola, R.D.; Universidad Nacional de Rosario; Rosario Univ. Nacional

    1996-01-01

    Single electron capture from H, He and H 2 targets by impact of Be 4+ and B 5+ projectiles is studied for intermediate and high collision energies. Total cross-sections are calculated using the continuum distorted wave-eikonal final state model. Theoretical results corresponding to capture to selective final bound states and to all final states are presented for impact energies ranging from 50 keV/amu to 3 MeV/amu. A comparison with available experimental data is also shown. (orig.)

  9. Targets and teamwork

    DEFF Research Database (Denmark)

    Skinner, Timothy C.; Lange, Karin S.; Hoey, Hilary

    2017-01-01

    with less disagreement about recommended targets. Multiple regression analysis indicated that teams reporting higher HbA1c targets and more target disagreement had parents reporting higher treatment targets. This seemed to partially account for center differences in Hb1Ac. Conclusions: The diabetes care...

  10. Correlation filter design using a single cluttered training image for detecting a noisy target in a nonoverlapping scene

    Science.gov (United States)

    Aguilar-González, Pablo Mario; Kober, Vitaly

    2010-08-01

    Classical correlation filters for object detection and location estimation are designed under the assumption that the shape and intensity values of the object of interest are explicitly known. In this work we assume that the target is given at unknown coordinates in a reference image with a cluttered background corrupted by additive noise. We consider the nonoverlapping signal model for both the reference image and the input scene. Optimal correlation filters, with respect to signal-to-noise ratio and peak-to-output energy, for object detection and location estimation are derived. Estimation techniques are proposed for the parameters required for filter design. Computer simulation results obtained with the proposed filters are presented and compared with those of common correlation filters.

  11. Precarity in the Brussels-Capital Region: The Issues at Stake for Public Policy in Response to the Precarization of Single-parent Families in Brussels

    Directory of Open Access Journals (Sweden)

    Martin Wagener

    2015-12-01

    Full Text Available The process of precarization is analysed in different manners in most literature concerning contemporary stakes of western welfare states. In comparison to other countries, Belgium has known a rather specific and delimited effect of the economic crisis from 2008, but certain groups face rather an ongoing effect of precarization on multiple and intertwined aspects. The aim of the article is to analyse the situation of precarity of single-parent families in the Brussels-Capital Region. The attention to that group is related to its higher risk of precarity and poverty. Furthermore it allows analysing the limits of public policies in the fight against poverty and precarity, that are clearly inadequate. Various options in terms of public policies and legal measures in the fight against poverty are discussed from three different angles of perspective: socioeconomic positions, social integration and the subjective experience (Dubet 1994. The tension between recognition and redistribution measures is central (Fraser 2011 in the argumentation for a balanced vision of policy measures to address single-parent poverty. La literatura científica sobre las apuestas contemporáneas de los estados de bienestar occidentales analiza de forma diferente el proceso de precarización. En comparación con otros países, Bélgica ha vivido desde 2008 un efecto de la crisis económica bastante específico y delimitado. Sin embargo, algunos grupos se enfrentan a un proceso de precarización continuo en numerosos aspectos, interrelacionados entre sí. El objetivo de este artículo es analizar la situación de precariedad de las familias monoparentales de la región de Bruselas capital. La atención a ese grupo está relacionada con su mayor riesgo de precariedad y pobreza. Además, permite analizar los límites de las políticas públicas en la lucha contra la pobreza y la precariedad, que son claramente insuficientes. Se analizan desde tres puntos de vista diversas

  12. Solid-state flow, mechanical alloying, and melt-related phenomena for [001] single-crystal tungsten ballistic rod penetrators interacting with steel targets

    Science.gov (United States)

    Pizana, Carlos

    This research program consists of a detailed microstructural investigation of in-target, single-crystal [001], clad (with Inconel 718) and unclad, W long-rod, ballistic penetrators. The rods were shot into rolled homogeneous armor (RHA) steel targets approximately 76 mm in thickness at impact velocities ranging from 1100 m/s to 1350 m/s. A comprehensive microstructural overview of the penetration process was obtained from this investigation. Solid-state flow/erosion, solid-state target/rod mixing as well as influencing factors such as strain rate, penetration performance, cladding interference and the interaction between target and projectile were emphasized. Some of the microstructural features observed, including deformation twins, cleaving, adiabatic shear bands and DRX support an overall solid-state penetration process. Furthermore they provide for a unifying perspective for the applicability of the hydrodynamic paradigm (DOP ≈ l∘rp/rt ) and earlier mechanistic erosion approaches. DRX and grain growth within adiabatic shear bands observed at specific high strain/strain-rate zones within the rods suggest that the projectile erodes by means of these microstructures in a solid-state form. This erosion process contributes to the performance of the rod by either allowing optimum flow of rod material which would increase penetration depth, or by maximizing rod material consumption which would reduce it. Since flow and/or erosion are also necessary in the target for perforation to occur, it is not surprising that the erosion process in the target was observed to mirror the one in the projectile. That is both target and projectile developed erosion zones with DRX facilitating the extreme deformation via dense overlapping shear band formation. Mechanical alloying and/or mixing of the target (steel) and rod (W, or W-Inconel 718) was also observed and investigated. Selective etching techniques as well as energy-dispersive x-ray mapping revealed unambiguous evidence of

  13. Control of established colon cancer xenografts using a novel humanized single chain antibody-streptococcal superantigen fusion protein targeting the 5T4 oncofetal antigen.

    Directory of Open Access Journals (Sweden)

    Kelcey G Patterson

    Full Text Available Superantigens (SAgs are microbial toxins that cross-link T cell receptors with major histocompatibility class II (MHC-II molecules leading to the activation of large numbers of T cells. Herein, we describe the development and preclinical testing of a novel tumor-targeted SAg (TTS therapeutic built using the streptococcal pyrogenic exotoxin C (SpeC SAg and targeting cancer cells expressing the 5T4 tumor-associated antigen (TAA. To inhibit potentially harmful widespread immune cell activation, a SpeC mutation within the high-affinity MHC-II binding interface was generated (SpeCD203A that demonstrated a pronounced reduction in mitogenic activity, yet this mutant could still induce immune cell-mediated cancer cell death in vitro. To target 5T4+ cancer cells, we engineered a humanized single chain variable fragment (scFv antibody to recognize 5T4 (scFv5T4. Specific targeting of scFv5T4 was verified. SpeCD203A fused to scFv5T4 maintained the ability to activate and induce immune cell-mediated cytotoxicity of colorectal cancer cells. Using a xenograft model of established human colon cancer, we demonstrated that the SpeC-based TTS was able to control the growth and spread of large tumors in vivo. This required both TAA targeting by scFv5T4 and functional SAg activity. These studies lay the foundation for the development of streptococcal SAgs as 'next-generation' TTSs for cancer immunotherapy.

  14. Peptide Mediated In Vivo Tumor Targeting of Nanoparticles through Optimization in Single and Multilayer In Vitro Cell Models

    Directory of Open Access Journals (Sweden)

    Celina Yang

    2018-03-01

    Full Text Available Optimizing the interface between nanoparticles (NPs and the biological environment at various levels should be considered for improving delivery of NPs to the target tumor area. For NPs to be successfully delivered to cancer cells, NPs needs to be functionalized for circulation through the blood vessels. In this study, accumulation of Polyethylene Glycol (PEG functionalized gold nanoparticles (GNPs was first tested using in vitro monolayer cells and multilayer cell models prior to in vivo models. A diameter of 10 nm sized GNP was selected for this study for sufficient penetration through tumor tissue. The surfaces of the GNPs were modified with PEG molecules, to improve circulation time by reducing non-specific uptake by the reticuloendothelial system (RES in animal models, and with a peptide containing integrin binding domain, RGD (arginyl-glycyl-aspartic acid, to improve internalization at the cellular level. A 10–12% accumulation of the injected GNP dose within the tumor was observed in vivo and the GNPs remained within the tumor tissue up to 72 h. This study suggests an in vitro platform for optimizing the accumulation of NP complexes in cells and tissue structures before testing them in animal models. Higher accumulation within the tumor in vivo upon surface modification is a promising outcome for future applications where GNPs can be used for drug delivery and radiation therapy.

  15. Haemodynamic consequences of targeted single- and dual-site right ventricular pacing in adults with congenital heart disease undergoing surgical pulmonary valve replacement

    Science.gov (United States)

    Plymen, Carla M.; Finlay, Malcolm; Tsang, Victor; O'leary, Justin; Picaut, Nathalie; Cullen, Shay; Walker, Fiona; Deanfield, John E; Hsia, T.Y.; Bolger, Aidan P.; Lambiase, Pier D.

    2015-01-01

    Aims The purpose of this study was to create an epicardial electroanatomic map of the right ventricle (RV) and then apply post-operative-targeted single- and dual-site RV temporary pacing with measurement of haemodynamic parameters. Cardiac resynchronization therapy is an established treatment for symptomatic left ventricular (LV) dysfunction. In congenital heart disease, RV dysfunction is a common cause of morbidity—little is known regarding the potential benefits of CRT in this setting. Methods and results Sixteen adults (age = 32 ± 8 years; 6 M, 10 F) with right bundle branch block (RBBB) and repaired tetralogy of Fallot (n = 8) or corrected congenital pulmonary stenosis (n = 8) undergoing surgical pulmonary valve replacement (PVR) for pulmonary regurgitation underwent epicardial RV mapping and haemodynamic assessment of random pacing configurations including the site of latest RV activation. The pre-operative pulmonary regurgitant fraction was 49 ± 10%; mean LV end-diastolic volume (EDV) 85 ± 19 mL/min/m2 and RVEDV 183 ± 89 mL/min/m2 on cardiac magnetic resonance imaging. The mean pre-operative QRS duration is 136 ± 26 ms. The commonest site of latest activation was the RV free wall and DDD pacing here alone or combined with RV apical pacing resulted in significant increases in cardiac output (CO) vs. AAI pacing (P < 0.01 all measures). DDDRV alternative site pacing significantly improved CO by 16% vs. AAI (P = 0.018), and 8.5% vs. DDDRV apical pacing (P = 0.02). Conclusion Single-site RV pacing targeted to the region of latest activation in patients with RBBB undergoing PVR induces acute improvements in haemodynamics and supports the concept of ‘RV CRT’. Targeted pacing in such patients has therapeutic potential both post-operatively and in the long term. PMID:25371427

  16. Murine CR1/2 targeted antigenized single-chain antibody fragments induce transient low affinity antibodies and negatively influence an ongoing immune response.

    Science.gov (United States)

    Prechl, József; Molnár, Eszter; Szekeres, Zsuzsanna; Isaák, Andrea; Papp, Krisztián; Balogh, Péter; Erdei, Anna

    2007-01-01

    We have generated a single-chain antibody which recognizes murine CR1/2 and carries a genetically fused influenza hemagglutinin derived peptide. Theoretically such a construct is able to crosslink the B cell antigen receptor and CR1/2 on peptide specific B cells. The construct was able to reach its CR1/2 positive target cells, yet intraperitoneal delivery of the construct elicited an IgM response only slightly exceeding that induced by the free peptide. Providing T cell help by the injection of peptide specific lymphocytes did not alter the response in essence, that is anti-peptide IgG was not detectable even after booster immunizations. When used as a booster vaccine following injection of the peptide in adjuvant, the construct even inhibited the development of IgG1 and IgG3 anti-peptide antibodies. These data indicate that although targeting of antigen to CR1/2 on B cells can enhance transient proliferation or differentiation of antigen specific B cells it cannot induce strong, longlasting humoral immune responses. Furthermore, CR1/2 targeting constructs may negatively influence an ongoing immune reaction.

  17. Perturbation of discrete sites on a single protein domain with RNA aptamers: targeting of different sides of the TATA-binding protein (TBP).

    Science.gov (United States)

    Hohmura, Ken I; Shi, Hua; Hirayoshi, Kazunori

    2013-01-01

    Control of interactions among proteins is critical in the treatment of diseases, but the specificity required is not easily incorporated into small molecules. Macromolecules could be more suitable as antagonists in this situation, and RNA aptamers have become particularly promising. Here we describe a novel selection procedure for RNA aptamers against a protein that constitutes a single structural domain, the Drosophila TATA-binding protein (TBP). In addition to the conventional filter partitioning method with free TBP as target, we performed another experiment, in which the TATA-bound form of TBP was targeted. Aptamers generated by both selections were able to bind specifically to TBP, but the two groups showed characteristics which were clearly different in terms of their capability to compete with TATA-DNA, their effects on the TATA-bound form of TBP, and their effects on in vitro transcription. The method used to generate these two groups of aptamers can be used with other targets to direct aptamer specificity to discrete sites on the surface of a protein.

  18. COMPARISON BETWEEN ABSORBED DOSES IN TARGET ORGANS IN PANORAMIC RADIOGRAPHY, USING SINGLE EMULSION AND DOUBLE EMULSION FILMS

    Directory of Open Access Journals (Sweden)

    A. R. Talaeipour

    2007-07-01

    Full Text Available "nThe use of panoramic radiography, due to its numerous advantages, is increasing. Radiographic films used in this technique are of double emulsion (DE type which are used with intensifying screens. Single emulsion (SE films can also be used. The purpose of this study was to determine the exposure parameters to achieve an appropriate optical density in these two types of films, and to estimate under such parameters, radiation doses to mandibular bone marrow (MBM, thyroid gland and parotid gland. This study was performed through a tissue equivalent phantom. First, with various tube voltage and tube current, 128 radiographs were taken of phantom with these two types of films. After examining the optical densities, the exposure parameters under which both films have the same density, were determined. Then, phantom again was exposed and MBM, thyroid gland and parotid gland absorbed doses were measured, using TLDs. It was demonstrated that: 1 SE films, in order to provide appropriate optical density, require two times radiation in comparison with double emulsion film; 2 using SE films increases MBM dose, up to 2-2.5 times, thyroid gland dose up to 1.7-2 times and parotid gland dose up to 1.3 times, in comparison with DE films; 3 in DE films, under lower exposure parameters and desirable processing, MBM dose up to 3.5 times, thyroid gland dose up to 1.5 times and parotid gland dose up to 2.5 times will increase. Considering that the risk of radiation induced cancers increases with repeated radiation doses, using SE films is not recommended.

  19. Pulsed irradiation improves target selectivity of infrared laser-evoked gene operator for single-cell gene induction in the nematode C. elegans.

    Science.gov (United States)

    Suzuki, Motoshi; Toyoda, Naoya; Takagi, Shin

    2014-01-01

    Methods for turning on/off gene expression at the experimenter's discretion would be useful for various biological studies. Recently, we reported on a novel microscope system utilizing an infrared laser-evoked gene operator (IR-LEGO) designed for inducing heat shock response efficiently in targeted single cells in living organisms without cell damage, thereby driving expression of a transgene under the control of a heat shock promoter. Although the original IR-LEGO can be successfully used for gene induction, several limitations hinder its wider application. Here, using the nematode Caenorhabditis elegans (C. elegans) as a subject, we have made improvements in IR-LEGO. For better spatial control of heating, a pulsed irradiation method using an optical chopper was introduced. As a result, single cells of C. elegans embryos as early as the 2-cell stage and single neurons in ganglia can be induced to express genes selectively. In addition, the introduction of site-specific recombination systems to IR-LEGO enables the induction of gene expression controlled by constitutive and cell type-specific promoters. The strategies adopted here will be useful for future applications of IR-LEGO to other organisms.

  20. Adolescents' Behaviors as Moderators for the Link between Parental Self-Efficacy and Parenting Practices.

    Science.gov (United States)

    Glatz, Terese; Cotter, Allison; Buchanan, Christy M

    2017-01-01

    Based on theory that parents with higher levels of self-efficacy (PSE) should find it easier to parent effectively in the face of challenging child behaviors than should parents with lower levels of PSE, this study examines the link between PSE and parenting using children's behaviors as potential moderators. Participants were 130 parents who had an older adolescent ( M age  = 17.58) in addition to the target adolescent ( M age  = 11.79), and both adolescents' externalizing behaviors were used as moderators for the link between PSE and parenting of the target adolescent. Path analysis in Mplus showed that higher PSE was linked to more promotive parenting but only among parents who had an older adolescent with lower levels of externalizing behaviors. Among parents of adolescents with higher levels of externalizing behaviors, whose promotive parenting was significantly lower than other parents overall, PSE did not predict promotive parenting. The link between PSE and parenting did not differ depending on the target adolescents' behavior. Findings suggest that the link between parents' beliefs and parenting depends on the broader family context. More specifically, how PSE is linked to parenting practices depends at least partly on the experiences that parents bring from parenting an older adolescent to their interactions with a later-born adolescent. From a clinical perspective, parents might need guidance in how to think about their earlier parenting experiences when parenting a younger adolescent.

  1. The Parenting to Reduce Adolescent Depression and Anxiety Scale: Assessing parental concordance with parenting guidelines for the prevention of adolescent depression and anxiety disorders

    Directory of Open Access Journals (Sweden)

    Mairead C. Cardamone-Breen

    2017-09-01

    Full Text Available Background Despite substantial evidence demonstrating numerous parental risk and protective factors for the development of adolescent depression and anxiety disorders, there is currently no single measure that assesses these parenting factors. To address this gap, we developed the Parenting to Reduce Adolescent Depression and Anxiety Scale (PRADAS as a criterion-referenced measure of parental concordance with a set of evidence-based parenting guidelines for the prevention of adolescent depression and anxiety disorders. In this paper, we used a sample of Australian parents of adolescents to: (1 validate the PRADAS as a criterion-referenced measure; (2 examine parental concordance with the guidelines in the sample; and (3 examine correlates of parental concordance with the guidelines. Methods Seven hundred eleven parents completed the PRADAS, as well as two established parenting measures, and parent-report measures of adolescent depression and anxiety symptoms. Six hundred sixty adolescent participants (aged 12–15 also completed the symptom measures. Concordance with the guidelines was assessed via nine subscale scores and a total score. Reliability of the scores was assessed with an estimate of the agreement coefficient, as well as 1-month test-retest reliability. Convergent validity was examined via correlations between the scale and two established parenting measures. Results One proposed subscale was removed from the final version of the scale, resulting in a total of eight subscales. Reliability was high for the total score, and acceptable to high for seven of the eight subscales. One-month test-retest reliability was acceptable to high for the total score. Convergent validity was supported by moderate to high correlations with two established measures of parenting. Overall, rates of parental concordance with the guidelines were low in our sample. Higher scores were associated with being female and higher levels of parental education

  2. Quantitative Single-Particle Digital Autoradiography with α-Particle Emitters for Targeted Radionuclide Therapy using the iQID Camera

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Brian W.; Frost, Sophia; Frayo, Shani; Kenoyer, Aimee L.; Santos, E. B.; Jones, Jon C.; Green, Damian J.; Hamlin, Donald K.; Wilbur, D. Scott; Fisher, Darrell R.; Orozco, Johnnie J.; Press, Oliver W.; Pagel, John M.; Sandmaier, B. M.

    2015-07-01

    Abstract Alpha emitting radionuclides exhibit a potential advantage for cancer treatments because they release large amounts of ionizing energy over a few cell diameters (50–80 μm) causing localized, irreparable double-strand DNA breaks that lead to cell death. Radioimmunotherapy (RIT) approaches using monoclonal antibodies labeled with alpha emitters may inactivate targeted cells with minimal radiation damage to surrounding tissues. For accurate dosimetry in alpha-RIT, tools are needed to visualize and quantify the radioactivity distribution and absorbed dose to targeted and non-targeted cells, especially for organs and tumors with heterogeneous radionuclide distributions. The aim of this study was to evaluate and characterize a novel single-particle digital autoradiography imager, iQID (ionizing-radiation Quantum Imaging Detector), for use in alpha-RIT experiments. Methods: The iQID camera is a scintillator-based radiation detection technology that images and identifies charged-particle and gamma-ray/X-ray emissions spatially and temporally on an event-by-event basis. It employs recent advances in CCD/CMOS cameras and computing hardware for real-time imaging and activity quantification of tissue sections, approaching cellular resolutions. In this work, we evaluated this system’s characteristics for alpha particle imaging including measurements of spatial resolution and background count rates at various detector configurations and quantification of activity distributions. The technique was assessed for quantitative imaging of astatine-211 (211At) activity distributions in cryosections of murine and canine tissue samples. Results: The highest spatial resolution was measured at ~20 μm full width at half maximum (FWHM) and the alpha particle background was measured at a rate of (2.6 ± 0.5) × 10–4 cpm/cm2 (40 mm diameter detector area). Simultaneous imaging of multiple tissue sections was performed using a large-area iQID configuration (ø 11.5 cm

  3. PET/CT-guided percutaneous liver mass biopsies and ablations: Targeting accuracy of a single 20 s breath-hold PET acquisition

    International Nuclear Information System (INIS)

    Shyn, P.B.; Tatli, S.; Sahni, V.A.; Sadow, C.A.; Forgione, K.; Mauri, G.; Morrison, P.R.; Catalano, P.J.; Silverman, S.G.

    2014-01-01

    Aim: To determine whether a single 20 s breath-hold positron-emission tomography (PET) acquisition obtained during combined PET/computed tomography (CT)-guided percutaneous liver biopsy or ablation procedures has the potential to target 2-[ 18 F]-fluoro-2-deoxy-D-glucose (FDG)-avid liver masses as accurately as up to 180 s breath-hold PET acquisitions. Materials and methods: This retrospective study included 10 adult patients with 13 liver masses who underwent FDG PET/CT-guided percutaneous biopsies (n = 5) or ablations (n = 5). PET was acquired as nine sequential 20 s, monitored, same-level breath-hold frames and CT was acquired in one monitored breath-hold. Twenty, 40, 60, and 180 s PET datasets were reconstructed. Two blinded readers marked tumour centres on randomized PET and CT datasets. Three-dimensional spatial localization differences between PET datasets and either 180 s PET or CT were analysed using multiple regression analyses. Statistical tests were two-sided and p < 0.05 was considered significant. Results: Targeting differences between 20 s PET and 180 s PET ranged from 0.7–20.3 mm (mean 5.3 ± 4.4 mm; median 4.3) and were not statistically different from 40 or 60 s PET (p = 0.74 and 0.91, respectively). Targeting differences between 20 s PET and CT ranged from 1.4–36 mm (mean 9.6 ± 7.1 mm; median 8.2 mm) and were not statistically different from 40, 60, or 180 s PET (p = 0.84, 0.77, and 0.35, respectively). Conclusion: Single 20 s breath-hold PET acquisitions from PET/CT-guided percutaneous liver procedures have the potential to target FDG-avid liver masses with equivalent accuracy to 180 s summed, breath-hold PET acquisitions and may facilitate strategies that improve image registration and shorten procedure times

  4. Parental feeding practices predict authoritative, authoritarian, and permissive parenting styles.

    Science.gov (United States)

    Hubbs-Tait, Laura; Kennedy, Tay Seacord; Page, Melanie C; Topham, Glade L; Harrist, Amanda W

    2008-07-01

    Our goal was to identify how parental feeding practices from the nutrition literature link to general parenting styles from the child development literature to understand how to target parenting practices to increase effectiveness of interventions. Stand-alone parental feeding practices could be targeted independently. However, parental feeding practices linked to parenting styles require interventions treating underlying family dynamics as a whole. To predict parenting styles from feeding practices and to test three hypotheses: restriction and pressure to eat are positively related whereas responsibility, monitoring, modeling, and encouraging are negatively related to an authoritarian parenting style; responsibility, monitoring, modeling, and encouraging are positively related whereas restriction and pressure to eat are negatively related to an authoritative parenting style; a permissive parenting style is negatively linked with all six feeding practices. Baseline data of a randomized-controlled intervention study. Two hundred thirty-nine parents (93.5% mothers) of first-grade children (134 boys, 105 girls) enrolled in rural public schools. Parental responses to encouraging and modeling questionnaires and the Child Feeding Questionnaire, as well as parenting styles measured by the Parenting Styles and Dimensions Questionnaire. Correlation and regression analyses. Feeding practices explained 21%, 15%, and 8% of the variance in authoritative, authoritarian, and permissive parenting, respectively. Restriction, pressure to eat, and monitoring (negative) significantly predicted an authoritarian style (Hypothesis 1); responsibility, restriction (negative), monitoring, and modeling predicted an authoritative style (Hypothesis 2); and modeling (negative) and restriction significantly predicted a permissive style (Hypothesis 3). Parental feeding practices with young children predict general parenting styles. Interventions that fail to address underlying parenting styles

  5. Child and parental poor health among families at risk for hunger attending a community health center.

    Science.gov (United States)

    Miller, Elizabeth; Wieneke, Kristin M; Murphy, J Michael; Desmond, Sheila; Schiff, Andrew; Canenguez, Katia M; Kleinman, Ronald E

    2008-05-01

    Hunger is prevalent in low-income families. Community clinics offer an opportunity to identify families who experience hunger and to address hunger-related health problems. Parents of pediatric patients seen in an urban clinic completed a single question hunger screen. A subsample of parents participated in an interview. Patient and parental medical and mental health diagnoses were collected from medical records. Children from families reporting hunger were more likely than those from families not reporting hunger to be obese and to have more documented medical diagnoses. Parents reporting hunger were also more likely to have mental health problems noted and to describe poor health status. Hunger is associated with specific health problems among children and parents in a low-income community clinic. A simple screen to identify hungry families in clinics may assist in recognition of hunger's contribution to child and parental poor health and development of targeted interventions.

  6. Measurement of single-spin azimuthal asymmetries in semi-inclusive electroproduction of pions and kaons on a longitudinally polarised deuterium target

    Science.gov (United States)

    HERMES Collaboration; Airapetian, A.; Akopov, N.; Akopov, Z.; Amarian, M.; Ammosov, V. V.; Andrus, A.; Aschenauer, E. C.; Augustyniak, W.; Avakian, H.; Avakian, R.; Avetissian, A.; Avetissian, E.; Bailey, P.; Baturin, V.; Baumgarten, C.; Beckmann, M.; Belostotski, S.; Bernreuther, S.; Bianchi, N.; Blok, H. P.; Böttcher, H.; Borissov, A.; Bouwhuis, M.; Brack, J.; Brüll, A.; Bryzgalov, V.; Capitani, G. P.; Chiang, H. C.; Ciullo, G.; Contalbrigo, M.; Court, G. R.; Dalpiaz, P. F.; de Leo, R.; de Nardo, L.; de Sanctis, E.; Devitsin, E.; di Nezza, P.; Düren, M.; Ehrenfried, M.; Elalaoui-Moulay, A.; Elbakian, G.; Ellinghaus, F.; Elschenbroich, U.; Ely, J.; Fabbri, R.; Fantoni, A.; Fechtchenko, A.; Felawka, L.; Fox, B.; Franz, J.; Frullani, S.; Gärber, Y.; Gapienko, G.; Gapienko, V.; Garibaldi, F.; Garutti, E.; Gaskell, D.; Gavrilov, G.; Gharibyan, V.; Graw, G.; Grebeniouk, O.; Greeniaus, L. G.; Haeberli, W.; Hafidi, K.; Hartig, M.; Hasch, D.; Heesbeen, D.; Henoch, M.; Hertenberger, R.; Hesselink, W. H. A.; Hillenbrand, A.; Holler, Y.; Hommez, B.; Iarygin, G.; Ivanilov, A.; Izotov, A.; Jackson, H. E.; Jgoun, A.; Kaiser, R.; Kinney, E.; Kisselev, A.; Königsmann, K.; Kolster, H.; Kopytin, M.; Korotkov, V.; Kozlov, V.; Krauss, B.; Krivokhijine, V. G.; Lagamba, L.; Lapikás, L.; Laziev, A.; Lenisa, P.; Liebing, P.; Lindemann, T.; Lipka, K.; Lorenzon, W.; Ma, B.-Q.; Makins, N. C. R.; Marukyan, H.; Masoli, F.; Menden, F.; Mexner, V.; Meyners, N.; Mikloukho, O.; Miller, C. A.; Miyachi, Y.; Muccifora, V.; Nagaitsev, A.; Nappi, E.; Naryshkin, Y.; Nass, A.; Nowak, W.-D.; Oganessyan, K.; Ohsuga, H.; Orlandi, G.; Potashov, S.; Potterveld, D. H.; Raithel, M.; Reggiani, D.; Reimer, P. E.; Reischl, A.; Reolon, A. R.; Rith, K.; Rosner, G.; Rostomyan, A.; Ryckbosch, D.; Sanjiev, I.; Savin, I.; Scarlett, C.; Schäfer, A.; Schill, C.; Schnell, G.; Schüler, K. P.; Schwind, A.; Seidl, R.; Seibert, J.; Seitz, B.; Shanidze, R.; Shibata, T.-A.; Shutov, V.; Simani, M. C.; Sinram, K.; Stancari, M.; Statera, M.; Steffens, E.; Steijger, J. J. M.; Stewart, J.; Stösslein, U.; Tanaka, H.; Taroian, S.; Tchuiko, B.; Terkulov, A.; Tessarin, S.; Thomas, E.; Tkabladze, A.; Trzcinski, A.; Tytgat, M.; Urciuoli, G. M.; van der Nat, P.; van der Steenhoven, G.; van de Vyver, R.; Vetterli, M. C.; Vikhrov, V.; Vincter, M. G.; Visser, J.; Vogel, C.; Vogt, M.; Volmer, J.; Weiskopf, C.; Wendland, J.; Wilbert, J.; Wise, T.; Yen, S.; Yoneyama, S.; Zihlmann, B.; Zohrabian, H.; Zupranski, P.

    2003-06-01

    Single-spin asymmetries have been measured for semi-inclusive electroproduction of π+, π-, π0 and K+ mesons in deep-inelastic scattering off a longitudinally polarised deuterium target. The asymmetries appear in the distribution of the hadrons in the azimuthal angle /φ around the virtual photon direction, relative to the lepton scattering plane. The corresponding analysing powers in the /sinφ moment of the cross section are /0.012+/-0.002(stat.)+/-0.002(syst.) for π+, /0.006+/-0.003(stat.)+/-0.002(syst.) for π-, /0.021+/-0.005(stat.)+/-0.003(syst.) for π0 and /0.013+/-0.006(stat.)+/-0.003(syst.) for K+. The /sin2φ moments are compatible with zero for all particles.

  7. A Home Economist Speaks Out: Need for a Parenting Course.

    Science.gov (United States)

    Dolny, Candice

    1996-01-01

    Social trends indicating change in family structure and roles, more single-parent and blended families, and the important role of parents in socialization demonstrate the need for parenting education. A parenting course should include understanding of healthy family life and parental roles, parenting styles, child development, and parenting…

  8. Dose painting to treat single-lobe prostate cancer with hypofractionated high-dose radiation using targeted external beam radiation: Is it feasible?

    International Nuclear Information System (INIS)

    Amini, Arya; Westerly, David C.; Waxweiler, Timothy V.; Ryan, Nicole; Raben, David

    2015-01-01

    Targeted focal therapy strategies for treating single-lobe prostate cancer are under investigation. In this planning study, we investigate the feasibility of treating a portion of the prostate to full-dose external beam radiation with reduced dose to the opposite lobe, compared with full-dose radiation delivered to the entire gland using hypofractionated radiation. For 10 consecutive patients with low- to intermediate-risk prostate cancer, 2 hypofractionated, single-arc volumetric-modulated arc therapy (VMAT) plans were designed. The first plan (standard hypofractionation regimen [STD]) included the entire prostate gland, treated to 70 Gy delivered in 28 fractions. The second dose painting plan (DP) encompassed the involved lobe treated to 70 Gy delivered in 28 fractions, whereas the opposing, uninvolved lobe received 50.4 Gy in 28 fractions. Mean dose to the opposing neurovascular bundle (NVB) was considerably lower for DP vs STD, with a mean dose of 53.9 vs 72.3 Gy (p < 0.001). Mean penile bulb dose was 18.6 Gy for DP vs 19.2 Gy for STD (p = 0.880). Mean rectal dose was 21.0 Gy for DP vs 22.8 Gy for STD (p = 0.356). Rectum V 70 (the volume receiving ≥70 Gy) was 2.01% for DP vs 2.74% for STD (p = 0.328). Bladder V 70 was 1.69% for DP vs 2.78% for STD (p = 0.232). Planning target volume (PTV) maximum dose points were 76.5 and 76.3 Gy for DP and STD, respectively (p = 0.760). This study demonstrates the feasibility of using VMAT for partial-lobe prostate radiation in patients with prostate cancer involving 1 lobe. Partial-lobe prostate plans appeared to spare adjacent critical structures including the opposite NVB

  9. Quantitative targeted and retrospective data analysis of relevant pesticides, antibiotics and mycotoxins in bakery products by liquid chromatography-single-stage Orbitrap mass spectrometry.

    Science.gov (United States)

    De Dominicis, Emiliano; Commissati, Italo; Gritti, Elisa; Catellani, Dante; Suman, Michele

    2015-01-01

    In addition to 'traditional' multi-residue and multi-contaminant multiple reaction monitoring (MRM) mass spectrometric techniques devoted to quantifying a list of targeted compounds, the global food industry requires non-targeted methods capable of detecting other possible potentially hazardous compounds. Ultra-high-performance liquid chromatography combined with a single-stage Orbitrap high-resolution mass spectrometer (UHPLC-HRMS Exactive™-Orbitrap Technology) was successfully exploited for the complete selective and quantitative determination of 33 target compounds within three major cross categories (pesticides, antibiotics and mycotoxins) in bakery matrices (specifically milk, wheat flour and mini-cakes). Resolution was set at 50 000 full width at half maximum (FWHM) to achieve the right compromise between an adequate scan speed and selectivity, allowing for the limitations related to the necessary generic sample preparation approach. An exact mass with tolerance of 5 ppm and minimum peak threshold of 10 000 units were fixed as the main identification conditions, including retention time and isotopic pattern as additional criteria devoted to greatly reducing the risk of false-positive findings. The full validation for all the target analytes was performed: linearity, intermediate repeatability and recovery (28 analytes within 70-120%) were positively assessed; furthermore, limits of quantification between 5 and 100 µg kg(-1) (with most of the analytes having a limit of detection below 6 µg kg(-1)) indicate good performance, which is compatible with almost all the regulatory needs. Naturally contaminated and fortified mini-cakes, prepared through combined use of industrial and pilot plant production lines, were analysed at two different concentration levels, obtaining good overall quantitative results and providing preliminary indications of the potential of full-scan HRMS cluster analysis. The effectiveness of this analytical approach was also tested in

  10. Measurement of the Target-Normal Single-Spin Asymmetry in Deep-Inelastic Scattering from the Reaction 3He{uparrow}(e,e')X

    Energy Technology Data Exchange (ETDEWEB)

    Katich, Joseph; Qian, Xin; Zhao, Yuxiang; Allada, Kalyan; Aniol, Konrad; Annand, John; Averett, Todd; Benmokhtar, Fatiha; Bertozzi, William; Bradshaw, Elliott; Bosted, Peter; Camsonne, Alexandre; Canan, Mustafa; Cates, Gordon; Chen, Chunhua; Chen, Jian-Ping; Chen, Wei; Chirapatpimol, Khem; Chudakov, Eugene; Cisbani, Evaristo; Cornejo, Juan; Cusanno, Francesco; Dalton, Mark; Deconinck, Wouter; De Jager, Cornelis; De Leo, Raffaele; Deng, Xiaoyan; Deur, Alexandre; Ding, Huaibo; Dolph, Peter; Dutta, Chiranjib; Dutta, Dipangkar; El Fassi, Lamiaa; Frullani, Salvatore; Gao, Haiyan; Garibaldi, Franco; Gaskell, David; Gilad, Gilad; Gilman, Ronald; Glamazdin, Oleksandr; Golge, Serkan; Guo, Lei; Hamilton, David; Hansen, Jens-Ole; Higinbotham, Douglas; Holmstrom, Timothy; Huang, Jijun; Huang, Min; Ibrahim Abdalla, Hassan; Iodice, Mauro; Jin, Ge; Jones, Mark; Kelleher, Aidan; Kim, Wooyoung; Kolarkar, Ameya; Korsch, Wolfgang; LeRose, John; Li, Xiaomei; Li, Y; Lindgren, Richard; Liyanage, Nilanga; Long, Elena; Lu, Hai-jiang; Margaziotis, Demetrius; Markowitz, Pete; Marrone, Stefano; McNulty, Dustin; Meziani, Zein-Eddine; Michaels, Robert; Moffit, Bryan; Munoz Camacho, Carlos; Nanda, Sirish; Narayan, Amrendra; Nelyubin, Vladimir; Norum, Blaine; Oh, Yoomin; Osipenko, Mikhail; Parno, Diana; Peng, Jen-chieh; Phillips, Sarah; Posik, Matthew; Puckett, Andrew; Qiang, Yi; Rakhman, Abdurahim; Ransome, Ronald; Riordan, Seamus; Saha, Arunava; Sawatzky, Bradley; Schulte, Elaine; Shahinyan, Albert; Hashemi Shabestari, Mitra; Sirca, Simon; Stepanyan, Stepan; Subedi, Ramesh; Sulkosky, Vincent; Tang, Liguang; Tobias, William; Urciuoli, Guido; Vilardi, Ignazio; Wang, Kebin; Wang, Y; Wojtsekhowski, Bogdan; Yan, X; Yao, Huan; Ye, Yunxiu; Ye, Z; Yuan, Lulin; Zhan, Xiaohui; Zhang, Yi; Zhang, Y -W; Zhao, Bo; Zheng, Xiaochao; Zhu, Lingyan; Zhu, Xiaofeng; Zong, Xing

    2014-07-01

    We report the first measurement of the target single-spin asymmetry in deep-inelastic scattering from the inclusive reaction 3He{uparrow}(e,e')X on a 3He gas target polarized normal to the lepton plane. Assuming time-reversal invariance, this asymmetry is strictly zero in the Born approximation. The experiment, conducted at Jefferson Lab using a 5.89 GeV electron beam, covers a range of 1.72 GeV, which is non-zero at the 2.75sigma level. Theoretical calculations, which assume two-photon exchange with quasi-free quarks, predict a neutron asymmetry of O(10−4) when both photons couple to one quark, and O(10−2) for the photons coupling to different quarks. Our measured asymmetry agrees both in sign and magnitude with the prediction that uses input based on the Sivers transverse momentum distribution obtained from semi-inclusive deep-inelastic scattering.

  11. Qualitative Sybr Green real-time detection of single nucleotide polymorphisms responsible for target-site resistance in insect pests: the example of Myzus persicae and Musca domestica.

    Science.gov (United States)

    Puggioni, V; Chiesa, O; Panini, M; Mazzoni, E

    2017-02-01

    Chemical insecticides have been widely used to control insect pests, leading to the selection of resistant populations. To date, several single nucleotide polymorphisms (SNPs) have already been associated with insecticide resistance, causing reduced sensitivity to many classes of products. Monitoring and detection of target-site resistance is currently one of the most important factors for insect pest management strategies. Several methods are available for this purpose: automated and high-throughput techniques (i.e. TaqMan or pyrosequencing) are very costly; cheaper alternatives (i.e. RFLP or PASA-PCRs) are time-consuming and limited by the necessity of a final visualization step. This work presents a new approach (QSGG, Qualitative Sybr Green Genotyping) which combines the specificity of PASA-PCR with the rapidity of real-time PCR analysis. The specific real-time detection of Cq values of wild-type or mutant alleles (amplified used allele-specific primers) allows the calculation of ΔCqW-M values and the consequent identification of the genotypes of unknown samples, on the basis of ranges previously defined with reference clones. The methodology is applied here to characterize mutations described in Myzus persicae and Musca domestica and we demonstrate it represents a valid, rapid and cost-effective technique that can be adopted for monitoring target-site resistance in field populations of these and other insect species.

  12. Identification of Five Novel Salmonella Typhi-Specific Genes as Markers for Diagnosis of Typhoid Fever Using Single-Gene Target PCR Assays

    Directory of Open Access Journals (Sweden)

    Yuan Xin Goay

    2016-01-01

    Full Text Available Salmonella Typhi (S. Typhi causes typhoid fever which is a disease characterised by high mortality and morbidity worldwide. In order to curtail the transmission of this highly infectious disease, identification of new markers that can detect the pathogen is needed for development of sensitive and specific diagnostic tests. In this study, genomic comparison of S. Typhi with other enteric pathogens was performed, and 6 S. Typhi genes, that is, STY0201, STY0307, STY0322, STY0326, STY2020, and STY2021, were found to be specific in silico. Six PCR assays each targeting a unique gene were developed to test the specificity of these genes in vitro. The diagnostic sensitivities and specificities of each assay were determined using 39 S. Typhi, 62 non-Typhi Salmonella, and 10 non-Salmonella clinical isolates. The results showed that 5 of these genes, that is, STY0307, STY0322, STY0326, STY2020, and STY2021, demonstrated 100% sensitivity (39/39 and 100% specificity (0/72. The detection limit of the 5 PCR assays was 32 pg for STY0322, 6.4 pg for STY0326, STY2020, and STY2021, and 1.28 pg for STY0307. In conclusion, 5 PCR assays using STY0307, STY0322, STY0326, STY2020, and STY2021 were developed and found to be highly specific at single-gene target resolution for diagnosis of typhoid fever.

  13. Targeted gene expression without a tissue-specific promoter: creating mosaic embryos using laser-induced single-cell heat shock

    Science.gov (United States)

    Halfon, M. S.; Kose, H.; Chiba, A.; Keshishian, H.

    1997-01-01

    We have developed a method to target gene expression in the Drosophila embryo to a specific cell without having a promoter that directs expression in that particular cell. Using a digitally enhanced imaging system to identify single cells within the living embryo, we apply a heat shock to each cell individually by using a laser microbeam. A 1- to 2-min laser treatment is sufficient to induce a heat-shock response but is not lethal to the heat-shocked cells. Induction of heat shock was measured in a variety of cell types, including neurons and somatic muscles, by the expression of beta-galactosidase from an hsp26-lacZ reporter construct or by expression of a UAS target gene after induction of hsGAL4. We discuss the applicability of this technique to ectopic gene expression studies, lineage tracing, gene inactivation studies, and studies of cells in vitro. Laser heat shock is a versatile technique that can be adapted for use in a variety of research organisms and is useful for any studies in which it is desirable to express a given gene in only a distinct cell or clone of cells, either transiently or constitutively, at a time point of choice.

  14. Parenting intervention effects on parental depressive symptoms: examining the role of parenting and child behavior.

    Science.gov (United States)

    Wong, Jessie J; Gonzales, Nancy A; Montaño, Zorash; Dumka, Larry; Millsap, Roger E

    2014-06-01

    Parental depression is a major risk factor in child development. Growing research suggests parenting programs can positively impact parental depressive symptoms, although the specific mechanisms that explain these effects are unknown. The current study examined parenting mediated effects of a parenting program on mothers' and fathers' depressive symptoms, as well as the role of child behavior in linking parenting to reductions in depressive symptoms. The study samples included 494 mothers and 288 fathers of Mexican origin adolescents who participated in a randomized trial of the Bridges to High School Program/Proyecto Puentes a la Secundaria, a universal prevention and promotion intervention that included parent training but did not directly target parental depressive symptoms. Parenting mediator models tested program effects on parental depressive symptoms through changes in harsh and supportive parenting. Results showed a significant indirect intervention effect on maternal depressive symptoms through changes in mothers' harsh parenting. Next, child behavior models revealed a partial mediation effect of harsh parenting and a full mediation effect of supportive parenting on maternal depressive symptoms through mothers' reports of child externalizing symptoms. Indirect effects of fathers' harsh and supportive parenting on paternal depressive symptoms were also found through fathers' reports of child behavior. PsycINFO Database Record (c) 2014 APA, all rights reserved.

  15. Single Targeted Exon Mutation Creates a True Congenic Mouse for Competitive Hematopoietic Stem Cell Transplantation: The C57BL/6-CD45.1STEM Mouse

    Directory of Open Access Journals (Sweden)

    Francois E. Mercier

    2016-06-01

    Full Text Available Defining the molecular regulators of hematopoietic stem and progenitor cells (HSPCs requires in vivo functional analyses. Competitive bone marrow transplants (BMTs compare control and test HSPCs to demonstrate the functional role of a genetic change or chemical perturbation. Competitive BMT is enabled by antibodies that specifically recognize hematopoietic cells from congenic mouse strains due to variants of the cell surface protein CD45, designated CD45.1 and CD45.2. The current congenic competitor strain, B6.SJL-Ptprca Pepcb/BoyJ (CD45.1, has a substantial inherent disadvantage in competition against the C57BL/6 (CD45.2 strain, confounding experimental interpretation. Despite backcrossing, the congenic interval over which the B6.SJL-Ptprca Pepcb/BoyJ strain differs is almost 40 Mb encoding ∼300 genes. Here, we demonstrate that a single amino acid change determines the CD45.1 epitope. Further, we report on the single targeted exon mutant (STEM mouse strain, CD45.1STEM, which is functionally equivalent to CD45.2 cells in competitive BMT. This strain will permit the precise definition of functional roles for candidate genes using in vivo HSPC assays.

  16. Quantitative single-particle digital autoradiography with α-particle emitters for targeted radionuclide therapy using the iQID camera

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Brian W., E-mail: brian.miller@pnnl.gov [Pacific Northwest National Laboratory, Richland, Washington 99354 and College of Optical Sciences, The University of Arizona, Tucson, Arizona 85719 (United States); Frost, Sofia H. L.; Frayo, Shani L.; Kenoyer, Aimee L.; Santos, Erlinda; Jones, Jon C.; Orozco, Johnnie J. [Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 (United States); Green, Damian J.; Press, Oliver W.; Pagel, John M.; Sandmaier, Brenda M. [Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 and Department of Medicine, University of Washington, Seattle, Washington 98195 (United States); Hamlin, Donald K.; Wilbur, D. Scott [Department of Radiation Oncology, University of Washington, Seattle, Washington 98195 (United States); Fisher, Darrell R. [Dade Moeller Health Group, Richland, Washington 99354 (United States)

    2015-07-15

    Purpose: Alpha-emitting radionuclides exhibit a potential advantage for cancer treatments because they release large amounts of ionizing energy over a few cell diameters (50–80 μm), causing localized, irreparable double-strand DNA breaks that lead to cell death. Radioimmunotherapy (RIT) approaches using monoclonal antibodies labeled with α emitters may thus inactivate targeted cells with minimal radiation damage to surrounding tissues. Tools are needed to visualize and quantify the radioactivity distribution and absorbed doses to targeted and nontargeted cells for accurate dosimetry of all treatment regimens utilizing α particles, including RIT and others (e.g., Ra-223), especially for organs and tumors with heterogeneous radionuclide distributions. The aim of this study was to evaluate and characterize a novel single-particle digital autoradiography imager, the ionizing-radiation quantum imaging detector (iQID) camera, for use in α-RIT experiments. Methods: The iQID camera is a scintillator-based radiation detection system that images and identifies charged-particle and gamma-ray/x-ray emissions spatially and temporally on an event-by-event basis. It employs CCD-CMOS cameras and high-performance computing hardware for real-time imaging and activity quantification of tissue sections, approaching cellular resolutions. In this work, the authors evaluated its characteristics for α-particle imaging, including measurements of intrinsic detector spatial resolutions and background count rates at various detector configurations and quantification of activity distributions. The technique was assessed for quantitative imaging of astatine-211 ({sup 211}At) activity distributions in cryosections of murine and canine tissue samples. Results: The highest spatial resolution was measured at ∼20 μm full width at half maximum and the α-particle background was measured at a rate as low as (2.6 ± 0.5) × 10{sup −4} cpm/cm{sup 2} (40 mm diameter detector area

  17. Quantitative single-particle digital autoradiography with α-particle emitters for targeted radionuclide therapy using the iQID camera.

    Science.gov (United States)

    Miller, Brian W; Frost, Sofia H L; Frayo, Shani L; Kenoyer, Aimee L; Santos, Erlinda; Jones, Jon C; Green, Damian J; Hamlin, Donald K; Wilbur, D Scott; Fisher, Darrell R; Orozco, Johnnie J; Press, Oliver W; Pagel, John M; Sandmaier, Brenda M

    2015-07-01

    Alpha-emitting radionuclides exhibit a potential advantage for cancer treatments because they release large amounts of ionizing energy over a few cell diameters (50-80 μm), causing localized, irreparable double-strand DNA breaks that lead to cell death. Radioimmunotherapy (RIT) approaches using monoclonal antibodies labeled with α emitters may thus inactivate targeted cells with minimal radiation damage to surrounding tissues. Tools are needed to visualize and quantify the radioactivity distribution and absorbed doses to targeted and nontargeted cells for accurate dosimetry of all treatment regimens utilizing α particles, including RIT and others (e.g., Ra-223), especially for organs and tumors with heterogeneous radionuclide distributions. The aim of this study was to evaluate and characterize a novel single-particle digital autoradiography imager, the ionizing-radiation quantum imaging detector (iQID) camera, for use in α-RIT experiments. The iQID camera is a scintillator-based radiation detection system that images and identifies charged-particle and gamma-ray/x-ray emissions spatially and temporally on an event-by-event basis. It employs CCD-CMOS cameras and high-performance computing hardware for real-time imaging and activity quantification of tissue sections, approaching cellular resolutions. In this work, the authors evaluated its characteristics for α-particle imaging, including measurements of intrinsic detector spatial resolutions and background count rates at various detector configurations and quantification of activity distributions. The technique was assessed for quantitative imaging of astatine-211 ((211)At) activity distributions in cryosections of murine and canine tissue samples. The highest spatial resolution was measured at ∼20 μm full width at half maximum and the α-particle background was measured at a rate as low as (2.6 ± 0.5) × 10(-4) cpm/cm(2) (40 mm diameter detector area). Simultaneous imaging of multiple tissue sections was

  18. Developing an Emotional Intelligence Program Training and Study Its Effectiveness on Emotional Intelligence of Adolescents with Emotional and Behavioral Problems That Living in Single Parent Families

    Science.gov (United States)

    Motamedi, Farzaneh; Ghobari-Bonab, Bagher; Beh-pajooh, Ahmad; Yekta, Mohsen Shokoohi; Afrooz, Gholam Ali

    2017-01-01

    Development of children and adolescents' personality is strongly affected by their parents, and absence of one of them has an undesirable effect on their development, and makes them vulnerable to later psychological disorders and behavioral problems. The purpose of this study was to develop an emotional intelligence training program and to…

  19. Body image as a target of victimization by peers/parents: Development and validation of the Body Image Victimization Experiences Scale.

    Science.gov (United States)

    Duarte, Cristiana; Pinto-Gouveia, José

    2017-10-01

    This study developed and established the psychometric properties of the Body Image Victimization Experiences Scale (BIVES). The BIVES retrospectively assesses the frequency (Part A-frequency) and effect (Part B-impact) of victimization experiences pertaining to body image, perpetrated by peers and parents/caregivers in childhood and adolescence. Distinct samples of Portuguese women were recruited in 2013-2014: two nonclinical samples of the general population (n = 1,177), aged 18-60 years, and a clinical sample of patients with Binge Eating Disorder (BED; n = 73), aged 19-59 years. An exploratory factor analysis was conducted in 632 participants. A confirmatory factor analysis (CFA) and the scale's psychometric properties were tested in 545 participants. The ability of the BIVES to discriminate the clinical from a nonclinical sample was examined. The scale presented two factors indicating the sources of the victimization-peers and parents. CFA results confirmed the scale's structure. The BIVES presented very good internal consistency, construct and discriminant validity, good test-retest reliability, and was associated with related constructs, body image shame, and eating psychopathology. The scale adequately discriminated between the clinical sample and a nonclinical sample. The BIVES is a valid and reliable measure that allows for a comprehensive assessment of body image-related victimization experiences.

  20. Integrated molecular targeting of IGF1R and HER2 surface receptors and destruction of breast cancer cells using single wall carbon nanotubes

    Energy Technology Data Exchange (ETDEWEB)

    Shao Ning [Delaware MEMS and Nanotechnology Laboratory, Department of Electrical and Computer Engineering, University of Delaware, Newark, DE 19716 (United States); Lu Shaoxin [Delaware MEMS and Nanotechnology Laboratory, Department of Electrical and Computer Engineering, University of Delaware, Newark, DE 19716 (United States); Wickstrom, Eric [Department of Biochemistry and Molecular Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107 (United States); Panchapakesan, Balaji [Delaware MEMS and Nanotechnology Laboratory, Department of Electrical and Computer Engineering, University of Delaware, Newark, DE 19716 (United States)

    2007-08-08

    Molecular targeting and photodynamic therapy have shown great potential for selective cancer therapy. We hypothesized that monoclonal antibodies that are specific to the IGF1 receptor and HER2 cell surface antigens could be bound to single wall carbon nanotubes (SWCNT) in order to concentrate SWCNT on breast cancer cells for specific near-infrared phototherapy. SWCNT functionalized with HER2 and IGF1R specific antibodies showed selective attachment to breast cancer cells compared to SWCNT functionalized with non-specific antibodies. After the complexes were attached to specific cancer cells, SWCNT were excited by {approx}808 nm infrared photons at {approx}800 mW cm{sup -2} for 3 min. Viability after phototherapy was determined by Trypan blue exclusion. Cells incubated with SWCNT/non-specific antibody hybrids were still alive after photo-thermal treatment due to the lack of SWNT binding to the cell membrane. All cancerous cells treated with IGF1R and HER2 specific antibody/SWCNT hybrids and receiving infrared photons showed cell death after the laser excitation. Quantitative analysis demonstrated that all the cells treated with SWCNT/IGF1R and HER2 specific antibody complex were completely destroyed, while more than 80% of the cells with SWCNT/non-specific antibody hybrids remained alive. Following multi-component targeting of IGF1R and HER2 surface receptors, integrated photo-thermal therapy in breast cancer cells led to the complete destruction of cancer cells. Functionalizing SWCNT with antibodies in combination with their intrinsic optical properties can therefore lead to a new class of molecular delivery and cancer therapeutic systems.

  1. Pre-targeted immunodetection in glioma patients: tumour localization and single-photon emission tomography imaging of [[sup 99m]Tc ]PnAO-biotin

    Energy Technology Data Exchange (ETDEWEB)

    Paganelli, G. (INB-CNR, Milan Univ. (Italy). Dept. of Nuclear Medicine Scientific Inst. H San Raffaele, Milan (Italy)); Magnani, P. (INB-CNR, Milan Univ. (Italy). Dept. of Nuclear Medicine Scientific Inst. H San Raffaele, Milan (Italy)); Zito, F. (INB-CNR, Milan Univ. (Italy). Dept. of Nuclear Medicine Scientific Inst. H San Raffaele, Milan (Italy)); Lucignani, G. (INB-CNR, Milan Univ. (Italy). Dept. of Nuclear Medicine Scientific Inst. H San Raffaele, Milan (Italy)); Sudati, F. (INB-CNR, Milan Univ. (Italy). Dept. of Nuclear Medicine Scientific Inst. H San Raffaele, Milan (Italy)); Truci, G. (Div. of Neurology, Milan Univ. (Italy) Scientific Inst. H San Raffaele, Milan (Italy)); Motti, E. (Div. of Neurosurgery, Milan Univ. (Italy) Scientific Inst. H San Raffaele, Milan (Italy)); Terreni, M. (Dept. of Pathology, Scientific Inst. H San Raffaele, Milan (Italy)); Pollo, B. (Dept. of Pathology, Scientific Inst. G. Besta, Milan (Italy)); Giovanelli, M. (Div. of Neurosurgery, Milan

    1994-04-01

    We have developed a three-step pre-targeting method using the avidin-biotin system. The rationale of this technique consists in vivo labelling of biotinylated MoAbs targeted onto tumour deposits, when most of the unbound antibodies have been cleared from the bloodstream as avidin-bound complexes. The anti-tenascin MoAb BC2, specific for the majority of gliomas, was biotinylated and 1 mg was administered i.v. in 20 patients with histologically documented cerebral lesions. After 24-36 h, 5 mg avidin was injected i.v. followed 24 h later by a third i.v. injection of 0.2 mg PnAO-biotin labelled with 15-20 mCi technetium-99m. No evidence of toxicity was observed. Whole-body biodistribution was measured at 20 min, 3 h and 5 h post-injection. [[sup 99m]Tc]PnAO-biotin had a fast blood clearance and was primarily excreted through the biliary system. A dedicated single-photon emission tomography system was used to acquire brain tomographic images 1-2 h after the administration of [[sup 99m]Tc]PnAO-biotin. Tumours were detected in 15/18 glioma patients with a tumour to non-tumour ratio of up 14:1. This three-step method, based on the sequential adminsitration of anti-tenascin MoAb BC2, avidin and [[sup 99m]Tc]PnAO-biotin, can support computed tomography or magnetic resonance imaging for the diagnosis and follow-up of patients with glioma. (orig./MG)

  2. CRISPR-Cas9 genome editing of a single regulatory element nearly abolishes target gene expression in mice--brief report.

    Science.gov (United States)

    Han, Yu; Slivano, Orazio J; Christie, Christine K; Cheng, Albert W; Miano, Joseph M

    2015-02-01

    To ascertain the importance of a single regulatory element in the control of Cnn1 expression using CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) genome editing. The CRISPR/Cas9 system was used to produce 3 of 18 founder mice carrying point mutations in an intronic CArG box of the smooth muscle cell-restricted Cnn1 gene. Each founder was bred for germline transmission of the mutant CArG box and littermate interbreeding to generate homozygous mutant (Cnn1(ΔCArG/ΔCArG)) mice. Quantitative reverse transcription polymerase chain reaction, Western blotting, and confocal immunofluorescence microscopy showed dramatic reductions in Cnn1 mRNA and CNN1 protein expression in Cnn1(ΔCArG/ΔCArG) mice with no change in other smooth muscle cell-restricted genes and little evidence of off-target edits elsewhere in the genome. In vivo chromatin immunoprecipitation assay revealed a sharp decrease in binding of serum response factor to the mutant CArG box. Loss of CNN1 expression was coincident with an increase in Ki-67 positive cells in the normal vessel wall. CRISPR/Cas9 genome editing of a single CArG box nearly abolishes Cnn1 expression in vivo and evokes increases in smooth muscle cell DNA synthesis. This facile genome editing system paves the way for a new generation of studies designed to test the importance of individual regulatory elements in living animals, including regulatory variants in conserved sequence blocks linked to human disease. © 2014 American Heart Association, Inc.

  3. Cosas de mujeres: familias monoparentales dominicanas en Barcelona y Nueva York Women’s issues: Dominican single-parent families in Barcelona and New York

    Directory of Open Access Journals (Sweden)

    Rosalina Alcalde

    2011-11-01

    Full Text Available Numerosos estudios a escala internacional y española avalan en la actualidad el riesgo de pobreza que presentan los hogares monoparentales encabezados por mujeres en las sociedades contemporáneas desarrolladas. Este artículo recoge  algunos de los principales resultados de sendas investigaciones llevadas a cabo en las ciudades de Barcelona y Nueva York sobre la incidencia de estas formas de hogar entre las mujeres inmigrantes, focalizando el análisis en las mujeres dominicanas.Several international and Spanish studies have shown that one-parent families are at considerable risk of poverty in contemporary and developed societies, particularly when the mother is the head, which is usually the case. This paper  provides the main results of some research carried out in Barcelona and New York about one-parent families and immigrant women, with particular emphasis on Dominican women.

  4. ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells

    DEFF Research Database (Denmark)

    Riso, Vincenzo; Cammisa, Marco; Kukreja, Harpreet

    2016-01-01

    ZFP57 is necessary for maintaining repressive epigenetic modifications at Imprinting control regions (ICRs). In mouse embryonic stem cells (ESCs), ZFP57 binds ICRs (ICRBS) and many other loci (non-ICRBS). To address the role of ZFP57 on all its target sites, we performed high-throughput and multi...

  5. Single site-specific integration targeting coupled with embryonic stem cell differentiation provides a high-throughput alternative to in vivo enhancer analyses

    Directory of Open Access Journals (Sweden)

    Adam C. Wilkinson

    2013-10-01

    Comprehensive analysis of cis-regulatory elements is key to understanding the dynamic gene regulatory networks that control embryonic development. While transgenic animals represent the gold standard assay, their generation is costly, entails significant animal usage, and in utero development complicates time-course studies. As an alternative, embryonic stem (ES cells can readily be differentiated in a process that correlates well with developing embryos. Here, we describe a highly effective platform for enhancer assays using an Hsp68/Venus reporter cassette that targets to the Hprt locus in mouse ES cells. This platform combines the flexibility of Gateway® cloning, live cell trackability of a fluorescent reporter, low background and the advantages of single copy insertion into a defined genomic locus. We demonstrate the successful recapitulation of tissue-specific enhancer activity for two cardiac and two haematopoietic enhancers. In addition, we used this assay to dissect the functionality of the highly conserved Ets/Ets/Gata motif in the Scl+19 enhancer, which revealed that the Gata motif is not required for initiation of enhancer activity. We further confirmed that Gata2 is not required for endothelial activity of the Scl+19 enhancer using Gata2−/− Scl+19 transgenic embryos. We have therefore established a valuable toolbox to study gene regulatory networks with broad applicability.

  6. HSA-based multi-target combination therapy: regulating drugs' release from HSA and overcoming single drug resistance in a breast cancer model.

    Science.gov (United States)

    Gou, Yi; Zhang, Zhenlei; Li, Dongyang; Zhao, Lei; Cai, Meiling; Sun, Zhewen; Li, Yongping; Zhang, Yao; Khan, Hamid; Sun, Hongbing; Wang, Tao; Liang, Hong; Yang, Feng

    2018-11-01

    Multi-drug delivery systems, which may be promising solution to overcome obstacles, have limited the clinical success of multi-drug combination therapies to treat cancer. To this end, we used three different anticancer agents, Cu(BpT)Br, NAMI-A, and doxorubicin (DOX), to build human serum albumin (HSA)-based multi-drug delivery systems in a breast cancer model to investigate the therapeutic efficacy of overcoming single drug (DOX) resistance to cancer cells in vivo, and to regulate the drugs' release from HSA. The HSA complex structure revealed that NAMI-A and Cu(BpT)Br bind to the IB and IIA sub-domain of HSA by N-donor residue replacing a leaving group and coordinating to their metal centers, respectively. The MALDI-TOF mass spectra demonstrated that one DOX molecule is conjugated with lysine of HSA by a pH-sensitive linker. Furthermore, the release behavior of three agents form HSA can be regulated at different pH levels. Importantly, in vivo results revealed that the HSA-NAMI-A-Cu(BpT)Br-DOX complex not only increases the targeting ability compared with a combination of the three agents (the NAMI-A/Cu(BpT)Br/DOX mixture), but it also overcomes DOX resistance to drug-resistant breast cancer cell lines.

  7. Single Photon Emission Computed Tomography/Positron Emission Tomography Imaging and Targeted Radionuclide Therapy of Melanoma: New Multimodal Fluorinated and Iodinated Radiotracers

    International Nuclear Information System (INIS)

    Maisonial, A.; Papon, J.; Bayle, M.; Vidal, A.; Auzeloux, Ph.; Rbah, L.; Bonnet-Duquennoy, M.; Miot-Noirault, E.; Galmier, M.J.; Borel, M.; Madelmont, J.C.; Moins, N.; Chezal, J.M.; Kuhnast, B.; Boisgard, R.; Dolle, F.; Tavitian, B.; Boisgard, R.; Tavitian, B.; Askienazy, S.

    2011-01-01

    This study reports a series of 14 new iodinated and fluorinated compounds offering both early imaging ( 123 I, 124 I, 18 F) and systemic treatment ( 131 I) of melanoma potentialities. The biodistribution of each 125 I-labeled tracer was evaluated in a model of melanoma B16F0-bearing mice, using in vivo serial γ scintigraphic imaging. Among this series, [ 125 I]56 emerged as the most promising compound in terms of specific tumoral uptake and in vivo kinetic profile. To validate our multimodality concept, the radiosynthesis of [ 18 F]56 was then optimized and this radiotracer has been successfully investigated for in vivo PET imaging of melanoma in B16F0- and B16F10-bearing mouse model. The therapeutic efficacy of [ 131 I]56 was then evaluated in mice bearing subcutaneous B16F0 melanoma, and a significant slow down in tumoral growth was demonstrated. These data support further development of 56 for PET imaging ( 18 F, 124 I) and targeted radionuclide therapy ( 131 I) of melanoma using a single chemical structure. (authors)

  8. Development of an enhanced bovine viral diarrhea virus subunit vaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells.

    Science.gov (United States)

    Pecora, Andrea; Malacari, Darío A; Pérez Aguirreburualde, María S; Bellido, Demian; Escribano, José M; Dus Santos, María J; Wigdorovitz, Andrés

    2015-01-01

    Bovine viral diarrhea virus (BVDV) is an important cause of economic losses worldwide. E2 is an immunodominant protein and a promising candidate to develop subunit vaccines. To improve its immunogenicity, a truncated E2 (tE2) was fused to a single chain antibody named APCH, which targets to antigen-presenting cells. APCH-tE2 and tE2 proteins were expressed in the baculovirus system and their immunogenicity was firstly compared in guinea pigs. APCH-tE2 vaccine was the best one to evoke a humoral response, and for this reason, it was selected for a cattle vaccination experiment. All the bovines immunized with 1.5 μg of APCH-tE2 developed high levels of neutralizing antibodies against BVDV up to a year post-immunization, demonstrating its significant potential as a subunit vaccine. This novel vaccine is undergoing scale-up and was transferred to the private sector. Nowadays, it is being evaluated for registration as the first Argentinean subunit vaccine for cattle. Copyright © 2014 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

  9. Characterization of an entomopathogenic fungi target integument protein, Bombyx mori single domain von Willebrand factor type C, in the silkworm, Bombyx mori.

    Science.gov (United States)

    Han, F; Lu, A; Yuan, Y; Huang, W; Beerntsen, B T; Huang, J; Ling, E

    2017-06-01

    The insect cuticle works as the first line of defence to protect insects from pathogenic infections and water evaporation. However, the old cuticle must be shed in order to enter the next developmental stage. During each ecdysis, moulting fluids are produced and secreted into the area among the old and new cuticles. In a previous study, the protein Bombyx mori single domain von Willebrand factor type C (BmSVWC; BGIBMGA011399) was identified in the moulting fluids of Bo. mori and demonstrated to regulate ecdysis. In this study we show that in Bo. mori larvae, BmSVWC primarily locates to the integument (epidermal cells and cuticle), wing discs and head. During the moulting stage, BmSVWC is released into the moulting fluids, and is then produced again by epidermal cells after ecdysis. Fungal infection was shown to decrease the amount of BmSVWC in the cuticle, which indicates that BmSVWC is a target protein of entomopathogenic fungi. Thus, BmSVWC is mainly involved in maintaining the integrity of the integument structure, which serves to protect insects from physical damage and pathogenic infection. © 2017 The Royal Entomological Society.

  10. Effective Parenting

    Science.gov (United States)

    ... Listen Español Text Size Email Print Share Effective Parenting Page Content Article Body Now that our children ... school play and his soccer games. Your Current Parenting Experiences Spend some time thinking about how you ...

  11. Working Parents

    Science.gov (United States)

    ... working-parent families are no longer exceptional. The Impact of Working When both parents are occupied with ... and perform a relaxation exercise. Or during your coffee breaks, forgo coffee and doughnuts and take a ...

  12. The Effect of a Parent Education Program on Selected Aspects of Parental Behavior: An Exploratory Study.

    Science.gov (United States)

    Shade, Daniel David

    The purpose of this exploratory study was to measure the impact of a specific style of parent education on parental behavior with their children. Six families, chosen by invitation from among parents participating in the Carbon County, Utah parent education programs, participated in the study. A BAB-ABBA single case experimental design was…

  13. Parent/Adolescent Weight Status Concordance and Parent Feeding Practices

    Science.gov (United States)

    Berge, Jerica M.; Meyer, Craig S.; Loth, Katie; MacLehose, Richard; Neumark-Sztainer, Dianne

    2017-01-01

    Background Prior studies have examined the independent influence of mothers’ weight status or childs’ weight status on parents’ use of specific feeding practices (i.e., restriction, pressure-to-eat). However, studies have not examined the mutual influence of parents' and adolescents' weight status on parents' feeding practices. This study examines the relationship between parent/adolescent weight status concordance and discordance and parent feeding practices. Methods Data from two linked population-based studies, EAT 2010 and F-EAT, were used for cross-sectional analysis. Mothers and fathers (n = 3,252; 63% females; mean age = 42.6 years) and adolescents (n = 2,153; 54% females; mean age = 14.4 years) were socio-economically and racially/ethnically diverse. Anthropometric assessments and surveys were completed at school by adolescents and surveys were completed at home by parents. Results Parents used the highest levels of pressure-to-eat feeding practices when parents and adolescents were both nonoverweight compared to all other combinations of concordant and discordant parent/adolescent weight status categories. Additionally, parents used the highest levels of food restriction when parents and adolescents were both overweight/obese compared to all other combinations of concordant and discordant parent/adolescent weight status categories. Sensitivity analyses with two parent households revealed similar patterns. Conclusions Results suggest that parents used parent feeding practices in response to both their adolescent’s and their own weight status. Results may inform health care providers and public health interventionists which parent/adolescent dyads are at highest risk for experiencing food restriction or pressure-to-eat parent feeding practices in the home environment and who to target in interventions. PMID:26304822

  14. The relationship between parental depressive symptoms, family type and adolescent functioning

    NARCIS (Netherlands)

    Sieh, D.S.; Visser-Meily, J.M.A.; Meijer, A.M.

    2013-01-01

    It is evident that parental depressive symptoms negatively influence adolescent behavior and various psychosocial outcomes. Certain family types like families with a chronically ill parent and single parent families are more vulnerable to parental depressive symptoms. However, the relationship

  15. An interdimensional correlation framework for real-time estimation of six degree of freedom target motion using a single x-ray imager during radiotherapy

    Science.gov (United States)

    Nguyen, D. T.; Bertholet, J.; Kim, J.-H.; O'Brien, R.; Booth, J. T.; Poulsen, P. R.; Keall, P. J.

    2018-01-01

    Increasing evidence suggests that intrafraction tumour motion monitoring needs to include both 3D translations and 3D rotations. Presently, methods to estimate the rotation motion require the 3D translation of the target to be known first. However, ideally, translation and rotation should be estimated concurrently. We present the first method to directly estimate six-degree-of-freedom (6DoF) motion from the target’s projection on a single rotating x-ray imager in real-time. This novel method is based on the linear correlations between the superior-inferior translations and the motion in the other five degrees-of-freedom. The accuracy of the method was evaluated in silico with 81 liver tumour motion traces from 19 patients with three implanted markers. The ground-truth motion was estimated using the current gold standard method where each marker’s 3D position was first estimated using a Gaussian probability method, and the 6DoF motion was then estimated from the 3D positions using an iterative method. The 3D position of each marker was projected onto a gantry-mounted imager with an imaging rate of 11 Hz. After an initial 110° gantry rotation (200 images), a correlation model between the superior-inferior translations and the five other DoFs was built using a least square method. The correlation model was then updated after each subsequent frame to estimate 6DoF motion in real-time. The proposed algorithm had an accuracy (±precision) of  -0.03  ±  0.32 mm, -0.01  ±  0.13 mm and 0.03  ±  0.52 mm for translations in the left-right (LR), superior-inferior (SI) and anterior-posterior (AP) directions respectively; and, 0.07  ±  1.18°, 0.07  ±  1.00° and 0.06  ±  1.32° for rotations around the LR, SI and AP axes respectively on the dataset. The first method to directly estimate real-time 6DoF target motion from segmented marker positions on a 2D imager was devised. The algorithm was evaluated using 81

  16. Parenting and Digital Media.

    Science.gov (United States)

    Coyne, Sarah M; Radesky, Jenny; Collier, Kevin M; Gentile, Douglas A; Linder, Jennifer Ruh; Nathanson, Amy I; Rasmussen, Eric E; Reich, Stephanie M; Rogers, Jean

    2017-11-01

    Understanding the family dynamic surrounding media use is crucial to our understanding of media effects, policy development, and the targeting of individuals and families for interventions to benefit child health and development. The Families, Parenting, and Media Workgroup reviewed the relevant research from the past few decades. We find that child characteristics, the parent-child relationship, parental mediation practices, and parents' own use of media all can influence children's media use, their attitudes regarding media, and the effects of media on children. However, gaps remain. First, more research is needed on best practices of parental mediation for both traditional and new media. Ideally, this research will involve large-scale, longitudinal studies that manage children from infancy to adulthood. Second, we need to better understand the relationship between parent media use and child media use and specifically how media may interfere with or strengthen parent-child relationships. Finally, longitudinal research on how developmental processes and individual child characteristics influence the intersection between media and family life is needed. The majority of children's media use takes place within a wider family dynamic. An understanding of this dynamic is crucial to understanding child media use as a whole. Copyright © 2017 by the American Academy of Pediatrics.

  17. Identification of Splice Variants, Targeted MicroRNAs and Functional Single Nucleotide Polymorphisms of the BOLA-DQA2 Gene in Dairy Cattle

    Science.gov (United States)

    Hou, Qinlei; Huang, Jinming; Ju, Zhihua; Li, Qiuling; Li, Liming; Wang, Changfa; Sun, Tao; Wang, Lingling; Hou, Minghai

    2012-01-01

    Major histocompatibility complex, class II, DQ alpha 2, also named BOLA-DQA2, belongs to the Bovine Leukocyte Antigen (BOLA) class II genes which are involved in the immune response. To explore the variability of the BOLA-DQA2 gene and resistance to mastitis in cows, the splice variants (SV), targeted microRNAs (miRNAs), and single nucleotide polymorphisms (SNPs) were identified in this study. A new SV (BOLA-DQA2-SV1) lacking part of exon 3 (195 bp) and two 3′-untranslated regions (UTR) (52 bp+167 bp) of the BOLA-DQA2 gene was found in the healthy and mastitis-infected mammary gland tissues. Four of 13 new SNPs and multiple nucleotide polymorphisms resulted in amino acid changes in the protein and SNP (c. +1283 C>T) may affect the binding to the seed sequence of bta-miR-2318. Further, we detected the relative expressions of two BOLA-DQA2 transcripts and five candidated microRNAs binding to the 3′-UTR of two transcripts in the mammary gland tissues in dairy cattle by using the quantitative real-time polymerase chain reaction. The result showed that expression of the BOLA-DQA2-SV1 mRNA was significantly upregulated 2.67-fold (pmastitis-infected mammary tissues (n=5) compared with the healthy mammary gland mammary tissues (n=5). Except for bta-miR-1777a, miRNA expression (bta-miR-296, miR-2430, and miR-671) was upregulated 1.75 to 2.59-fold (pmastitis cows. Our findings reveal that BOLA-DQA2-SV1 may play an important role in the mastitis resistance in dairy cattle. Whether the SNPs affect the structure of the BOLA-DQA2 gene or association with mastitis resistance is unknown and warrants further investigation. PMID:22084936

  18. Identification of singles bar as a direct transcriptional target of Drosophila Myocyte enhancer factor-2 and a regulator of adult myoblast fusion.

    Science.gov (United States)

    Brunetti, Tonya M; Fremin, Brayon J; Cripps, Richard M

    2015-05-15

    In Drosophila, myoblast fusion is a conserved process in which founder cells (FCs) and fusion competent myoblasts (FCMs) fuse to form a syncytial muscle fiber. Mutants for the myogenic regulator Myocyte enhancer factor-2 (MEF2) show a failure of myoblast fusion, indicating that MEF2 regulates the fusion process. Indeed, chromatin immunoprecipitation studies show that several genes involved in myoblast fusion are bound by MEF2 during embryogenesis. Of these, the MARVEL domain gene singles bar (sing), is down-regulated in MEF2 knockdown pupae, and has five consensus MEF2 binding sites within a 9000-bp region. To determine if MEF2 is an essential and direct regulator of sing during pupal muscle development, we identified a 315-bp myoblast enhancer of sing. This enhancer was active during myoblast fusion, and mutation of two MEF2 sites significantly decreased enhancer activity. We show that lack of sing expression resulted in adult lethality and muscle loss, due to a failure of fusion during the pupal stage. Additionally, we sought to determine if sing was required in either FCs or FCMs to support fusion. Interestingly, knockdown of sing in either population did not significantly affect fusion, however, knockdown in both FCs and FCMs resulted in muscles with significantly reduced nuclei numbers, provisionally indicating that sing function is required in either cell type, but not both. Finally, we found that MEF2 regulated sing expression at the embryonic stage through the same 315-bp enhancer, indicating that sing is a MEF2 target at both critical stages of myoblast fusion. Our studies define for the first time how MEF2 directly controls fusion at multiple stages of the life cycle, and provide further evidence that the mechanisms of fusion characterized in Drosophila embryos is also used in the formation of the more complex adult muscles. Copyright © 2015 Elsevier Inc. All rights reserved.

  19. Structural insight of dopamine β-hydroxylase, a drug target for complex traits, and functional significance of exonic single nucleotide polymorphisms.

    Directory of Open Access Journals (Sweden)

    Abhijeet Kapoor

    Full Text Available Human dopamine β-hydroxylase (DBH is an important therapeutic target for complex traits. Several single nucleotide polymorphisms (SNPs have also been identified in DBH with potential adverse physiological effect. However, difficulty in obtaining diffractable crystals and lack of a suitable template for modeling the protein has ensured that neither crystallographic three-dimensional structure nor computational model for the enzyme is available to aid rational drug design, prediction of functional significance of SNPs or analytical protein engineering.Adequate biochemical information regarding human DBH, structural coordinates for peptidylglycine alpha-hydroxylating monooxygenase and computational data from a partial model of rat DBH were used along with logical manual intervention in a novel way to build an in silico model of human DBH. The model provides structural insight into the active site, metal coordination, subunit interface, substrate recognition and inhibitor binding. It reveals that DOMON domain potentially promotes tetramerization, while substrate dopamine and a potential therapeutic inhibitor nepicastat are stabilized in the active site through multiple hydrogen bonding. Functional significance of several exonic SNPs could be described from a structural analysis of the model. The model confirms that SNP resulting in Ala318Ser or Leu317Pro mutation may not influence enzyme activity, while Gly482Arg might actually do so being in the proximity of the active site. Arg549Cys may cause abnormal oligomerization through non-native disulfide bond formation. Other SNPs like Glu181, Glu250, Lys239 and Asp290 could potentially inhibit tetramerization thus affecting function.The first three-dimensional model of full-length human DBH protein was obtained in a novel manner with a set of experimental data as guideline for consistency of in silico prediction. Preliminary physicochemical tests validated the model. The model confirms, rationalizes and

  20. Anti-CD20 single chain variable antibody fragment-apolipoprotein A-I chimera containing nanodisks promote targeted bioactive agent delivery to CD20-positive lymphomas.

    Science.gov (United States)

    Crosby, Natasha M; Ghosh, Mistuni; Su, Betty; Beckstead, Jennifer A; Kamei, Ayako; Simonsen, Jens B; Luo, Bing; Gordon, Leo I; Forte, Trudy M; Ryan, Robert O

    2015-08-01

    A fusion protein comprising an α-CD20 single chain variable fragment (scFv) antibody, a spacer peptide, and human apolipoprotein (apo) A-I was constructed and expressed in Escherichia coli. The lipid interaction properties intrinsic to apoA-I as well as the antigen recognition properties of the scFv were retained by the chimera. scFv•apoA-I was formulated into nanoscale reconstituted high-density lipoprotein particles (termed nanodisks; ND) and incubated with cultured cells. α-CD20 scFv•apoA-I ND bound to CD20-positive non-Hodgkins lymphoma (NHL) cells (Ramos and Granta) but not to CD20-negative T lymphocytes (i.e., Jurkat). Binding to NHL cells was partially inhibited by pre-incubation with rituximab, a monoclonal antibody directed against CD20. Confocal fluorescence microscopy analysis of Granta cells following incubation with α-CD20 scFv•apoA-I ND formulated with the intrinsically fluorescent hydrophobic polyphenol, curcumin, revealed α-CD20 scFv•apoA-I localizes to the cell surface, while curcumin off-loads and gains entry to the cell. Compared to control incubations, viability of cultured NHL cells was decreased upon incubation with α-CD20 scFv•apoA-I ND harboring curcumin. Thus, formulation of curcumin ND with α-CD20 scFv•apoA-I as the scaffold component confers cell targeting and enhanced bioactive agent delivery, providing a strategy to minimize toxicity associated with chemotherapeutic agents.

  1. Dinosaur Reproduction and Parenting

    Science.gov (United States)

    Horner, John R.

    Non-avian dinosaur reproductive and parenting behaviors were mostly similar to those of extant archosaurs. Non-avian dinosaurs were probably sexually dimorphic and some may have engaged in hierarchical rituals. Non-avian coelurosaurs (e.g. Troodontidae, Oviraptorosauria) had two active oviducts, each of which produced single eggs on a daily or greater time scale. The eggs of non-coelurosaurian dinosaurs (e.g. Ornithischia, Sauropoda) were incubated in soils, whereas the eggs of non-avian coelurosaurs (e.g. Troodon, Oviraptor) were incubated with a combination of soil and direct parental contact. Parental attention to the young was variable, ranging from protection from predators to possible parental feeding of nest-bound hatchlings. Semi-altricial hadrosaur hatchlings exited their respective nests near the time of their first linear doubling. Some reproductive behaviors, once thought exclusive to Aves, arose first in non-avian dinosaurs. The success of the Dinosauria may be related to reproductive strategies.

  2. Parenting Seminars for Divorcing Parents.

    Science.gov (United States)

    Frieman, Barry B.

    1995-01-01

    Profiles the parenting seminars and counseling services for divorcing parents offered by the Children of Separation and Divorce Center, a community service agency in Maryland. The seminars are designed to help parents adjust to divorce and understand the needs of their children during and after the divorce process. (MDM)

  3. Family structure and park use among parents.

    Science.gov (United States)

    Fan, Yingling; French, Simone A; Das, Kirti V

    2012-11-01

    Despite the increasingly diversified family structure in the U.S., little research examines differences in park use between nontraditional and traditional family structures. This study examines family-structure differences in parent park use. It was hypothesized that working single parents and dual-worker parents have lower levels of park use than parents in two-parent, single-worker families. Data from a 2010 park-use survey in three urban neighborhoods in Minneapolis MN (N=261 parents) were analyzed in 2012. Multiple variables of park use were developed, including recalled measures over the past 3 days and over the past year. Family-structure differences in these variables were examined using multivariate regression analyses. After controlling for spatial clustering effects and confounding factors, working single parents reported 32.6% (pparents in two-parent, single-worker families. Dual-worker parents did not report fewer park visits in the past 3 days than parents in two-parent, single-worker families, yet the length of time they spent in parks during these visits was 41.5% (pparents and dual-worker parents is needed in descriptive and intervention research aiming to promote park use among families with children. Copyright © 2012 American Journal of Preventive Medicine. Published by Elsevier Inc. All rights reserved.

  4. Associations of general parenting and parent-child relationship with pediatric obesity: a meta-analysis.

    Science.gov (United States)

    Pinquart, Martin

    2014-05-01

    The objective of the meta-analysis is to integrate available results on associations of general parenting (not specific to feeding and activity promotion) and parent-child relations with child weight status, eating, and physical activity. Searching in electronic databases and cross-referencing identified 156 empirical studies. Random-effects meta-analysis was computed. A positive parent-child relationship and higher levels of parental responsiveness were associated with lower weight, healthier eating, and more physical activity of the child. Parental demandingness, overprotection, psychological control, inconsistency, and parenting styles showed associations with some of the assessed outcome variables. Most effect sizes were small and varied by study characteristics. The small effects do not support making general parenting styles, parental demandingness, responsiveness, and the quality of the parent-child relationship a main target of preventing and treating obesity. Reducing parental inconsistency may be a better target if available results are replicated in future studies.

  5. Targeted Learning

    CERN Document Server

    van der Laan, Mark J

    2011-01-01

    The statistics profession is at a unique point in history. The need for valid statistical tools is greater than ever; data sets are massive, often measuring hundreds of thousands of measurements for a single subject. The field is ready to move towards clear objective benchmarks under which tools can be evaluated. Targeted learning allows (1) the full generalization and utilization of cross-validation as an estimator selection tool so that the subjective choices made by humans are now made by the machine, and (2) targeting the fitting of the probability distribution of the data toward the targe

  6. Prevention of Parent to Child Transmission of HIV: Single Centre Experience of 14 years at Tertiary Care Hospital in Delhi, India.

    Science.gov (United States)

    Radhika, A G; Chawla, Sonia; Bhaskaran, Sruthi

    2017-08-01

    Prevention of Parent To Child Transmission (PPTCT) of HIV/AIDS is an integral component of AIDS control programme. PPTCT is an ongoing programme since last 15 years. The aim of the study was to evaluate the reduction in the burden of disease in newborn and infants by prevention of parents to child transmission of HIV/AIDS. This retrospective study was conducted at Department of Obstetrics and Gynecology in a tertiary care hospital of Delhi from May 2002 to May 2015. The data was collected from records of maternal details registered at PPTCT clinic as well as list of infants undergoing Early Infant Diagnosis (EID) recorded in the standard format as per instructions from National AIDS Control Organization (NACO) of India. The Programme performance was assessed against performance indicators stated by NACO, India. Evaluation was done by dividing study period into two halves of seven years each. Out of 2,52,447 new antenatal case registration, overall, 43% received pretest of which, 91% were tested. Antenatal seropositivity rate varied from 0.1%-0.25%. Of 243 seropositive antenatal women 187 partners tested positive. While 25 women opted for MTP, 15 had still births. There were 17 neonatal deaths at 3-12 months attributable to respiratory infections and diarrheal diseases. Operative delivery rates declined from 50% to 31% over the years. Most women opted for breast feeding. The lost to follow up rate of newborns was quite high with details of only 43.5% being available at 18 months of infant's age. A total of three infants tested HIV positive at 18 months of age. The study highlights the practical aspects of policy implementation and operational issues involved in low resource country.

  7. Study Protocol of MINI SALTEN: a technology-based multi-component intervention in the school environment targeting healthy habits of first grade children and their parents.

    Science.gov (United States)

    Kovalskys, Irina; Rausch Herscovici, Cecile; Indart Rougier, Paula; De Gregorio, María José; Zonis, Luciana; Orellana, Liliana

    2017-05-06

    parsimonious model for each outcome will be reported. The False Discovery Rate criterion will be used to correct for multiple testing in non-planned analyses. It is a pioneer assessment of the impact of a technology-based virtual intervention and a school-based PA program, designed to prevent obesity, and involving the parents at public schools of Buenos Aires. Current Controlled Trials ISRCTN58093412 . Registered March 14th, 2016 (retrospectively registered).

  8. Do parents of adolescents request the same universal parental support as parents of younger children? A random sample of Swedish parents.

    Science.gov (United States)

    Thorslund, Karin; Johansson Hanse, Jan; Axberg, Ulf

    2017-07-01

    Universal parental support intended to enhance parents' capacity for parenting is an important aspect of public health strategies. However, support has mostly been aimed at parents, especially mothers, of younger children. There is a gap in the research concerning parents of adolescents and fathers' interest in parenting support. To investigate and compare the interest in parenting support of parents of adolescents and younger children, potential differences between mothers and fathers, and their knowledge of what is being offered to them already, and to explore their requirements for future universal parental support. Telephone interviews were conducted with a random sample of 1336 parents. Quantitative methods were used to analyze differences between groups and qualitative methods were used to analyze open-ended questions in regard to parents' requirements for future universal parental support. About 82% of the parents of adolescents interviewed think that offering universal parental support is most important during child's adolescence. There is a substantial interest, particularly among mothers, in most forms of support. Despite their interest, parents have limited awareness of the support available. Only 7% knew about the local municipality website, although 70% reported a possible interest in such a website. Similarly, 3% knew that a parent phone line was available to them, while 59% reported a possible interest. It poses a challenge but is nevertheless important for municipalities to develop support targeted at parents of adolescents which is tailored to their needs, and to reach out with information.

  9. [Parenting styles].

    Science.gov (United States)

    Torío López, Susana; Peña Calvo, José Vicente; Inda Caro, Mercedes

    2008-02-01

    Parental educational styles constitute one of the key elements of family socialization. The aim of the present essay is to present the results of a research project carried out in the Principality of Asturias (Spain) among 2,965 families with children of infant and primary-school age (5-8 years old). This research attempts to analyse, among other aspects, parental behaviour tendencies in child upbringing. The analysis of the results obtained allows us to: 1) identify the most common attitudinal and behavioural tendencies of parents in the upbringing of their children; 2) determine how many people have a well defined parental style, and delimit their socio-educational characteristics. Lastly, we consider the need to change some parental behaviour patterns and stress the importance of family education programmes, with the aim of promoting appropriate parenting models and modifying or improving current practices.

  10. Adoptive parenting.

    Science.gov (United States)

    Grotevant, Harold D; Lo, Albert Yh

    2017-06-01

    Challenges in adoptive parenting continue to emerge as adoption policies and practices evolve. We review three areas of research in adoptive parenting that reflect contemporary shifts in adoption. First, we highlight recent findings concerning openness in adoption contact arrangements, or contact between a child's families of birth and rearing. Second, we examine research regarding racial and cultural socialization in transracial and international adoptions. Finally, we review investigations of parenting experiences of lesbian and gay adoptive parents. Overall, parenting processes (e.g., supportive vs. problematic family interaction) are better predictors of child adjustment than are group differences (e.g., open vs. closed adoptions; adoption by heterosexual vs. same-sex parents). The distinctive needs of adopted children call for preparation of adoption-competent mental health, casework, education, and health care professionals. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. GAME (Goals - Activity - Motor Enrichment): protocol of a single blind randomised controlled trial of motor training, parent education and environmental enrichment for infants at high risk of cerebral palsy.

    Science.gov (United States)

    Morgan, Catherine; Novak, Iona; Dale, Russell C; Guzzetta, Andrea; Badawi, Nadia

    2014-10-07

    Cerebral palsy is the most common physical disability of childhood and early detection is possible using evidence based assessments. Systematic reviews indicate early intervention trials rarely demonstrate efficacy for improving motor outcomes but environmental enrichment interventions appear promising. This study is built on a previous pilot study and has been designed to assess the effectiveness of a goal - oriented motor training and enrichment intervention programme, "GAME", on the motor outcomes of infants at very high risk of cerebral palsy (CP) compared with standard community based care. A two group, single blind randomised controlled trial (n = 30) will be conducted. Eligible infants are those diagnosed with CP or designated "at high risk of CP" on the basis of the General Movements Assessment and/or abnormal neuroimaging. A physiotherapist and occupational therapist will deliver home-based GAME intervention at least fortnightly until the infant's first birthday. The intervention aims to optimize motor function and engage parents in developmental activities aimed at enriching the home learning environment. Primary endpoint measures will be taken 16 weeks after intervention commences with the secondary endpoint at 12 months and 24 months corrected age. The primary outcome measure will be the Peabody Developmental Motor Scale second edition. Secondary outcomes measures include the Gross Motor Function Measure, Bayley Scales of Infant and Toddler Development, Affordances in the Home Environment for Motor Development - Infant Scale, and the Canadian Occupational Performance Measure. Parent well-being will be monitored using the Depression Anxiety and Stress Scale. This paper presents the background, design and intervention protocol of a randomised trial of a goal driven, motor learning approach with customised environmental interventions and parental education for young infants at high risk of cerebral palsy. This trial is registered on the Australian

  12. Parental Bonding

    Directory of Open Access Journals (Sweden)

    T. Paul de Cock

    2014-08-01

    Full Text Available Estimating the early parent–child bonding relationship can be valuable in research and practice. Retrospective dimensional measures of parental bonding provide a means for assessing the experience of the early parent–child relationship. However, combinations of dimensional scores may provide information that is not readily captured with a dimensional approach. This study was designed to assess the presence of homogeneous groups in the population with similar profiles on parental bonding dimensions. Using a short version of the Parental Bonding Instrument (PBI, three parental bonding dimensions (care, authoritarianism, and overprotection were used to assess the presence of unobserved groups in the population using latent profile analysis. The class solutions were regressed on 23 covariates (demographics, parental psychopathology, loss events, and childhood contextual factors to assess the validity of the class solution. The results indicated four distinct profiles of parental bonding for fathers as well as mothers. Parental bonding profiles were significantly associated with a broad range of covariates. This person-centered approach to parental bonding has broad utility in future research which takes into account the effect of parent–child bonding, especially with regard to “affectionless control” style parenting.

  13. Authoritative parenting and drug-prevention practices: implications for antidrug ads for parents.

    Science.gov (United States)

    Stephenson, Michael T; Quick, Brian L; Atkinson, Joshua; Tschida, David A

    2005-01-01

    This research employed the theory of reasoned action to investigate the role of authoritative parenting in 3 drug-prevention behaviors: (a) parental monitoring, (b) parent-child discussions, and (c) awareness of the child's environment. A phone survey of 158 parents of adolescents in 7th, 9th, and 11th grades revealed that authoritative parenting was correlated with parenting practices that reduce the likelihood of adolescent drug use, including discussing family rules about drugs, discussing strategies to avoid drugs, discussing those in trouble with drugs, parental monitoring, knowing the child's plans for the coming day, and personally knowing the child's friends well. Additionally, authoritative parenting moderated the attitude-behavioral intention relation for parental monitoring and awareness of the child's environment, with the weakest relation detected for low-authoritative parents. The utility of these findings in helping design and target antidrug messages for parents more effectively is discussed.

  14. Single agent- and combination treatment with two targeted suicide gene therapy systems is effective in chemoresistant small cell lung cancer cells

    DEFF Research Database (Denmark)

    Michaelsen, Signe R; Christensen, Camilla L; Sehested, Maxwell

    2012-01-01

    Transcriptional targeted suicide gene (SG) therapy driven by the insulinoma-associated 1 (INSM1) promoter makes it possible to target suicide toxin production and cytotoxicity exclusively to small cell lung cancer (SCLC) cells and tumors. It remains to be determined whether acquired chemoresistance......, as observed in the majority of SCLC patients, desensitizes SCLC cells to INSM1 promoter-driven SG therapy....

  15. An introduction to parental alienation syndrome.

    Science.gov (United States)

    Farkas, Michelle M

    2011-04-01

    Parental alienation syndrome (PAS) can occur during a tumultuous divorce between embattled parents involved in a bitter child custody dispute. During parental warfare, a child is used as a weapon by one parent (alienating parent) against the other parent (alienated/targeted parent). The targeted parent-child relationship once encased with unconditional love is transformed by an unrelenting campaign of denigration, criticism, and hatred. Since nursing literature on PAS is almost nonexistent, the purpose of this article is to increase nursing awareness and provide basic information. Awareness of PAS symptoms and interpersonal dynamics is important to prompt nurses in recommending treatment for families. Nurses should collaboratively join other professionals in their quest to provide the best treatment possible. Copyright 2011, SLACK Incorporated.

  16. Parental divorce and parental death

    DEFF Research Database (Denmark)

    Marcussen, Jette; Thuen, Frode; Poul, Bruun

    2015-01-01

    The aim of this review was to identify research on children and adolescents who experience double bereavement, i.e. the experience of loss through parental divorce followed by either parental death or critical illness with imminent death. This knowledge may identify evidence to underpin knowledge......; challenges in both custodial and non-custodial parental death; risk of mental health problems, and the need of support and interventions....

  17. Do Parent Education Programs Promote Healthy Post-Divorce Parenting? Critical Distinctions and a Review of the Evidence

    Science.gov (United States)

    Sigal, Amanda; Sandler, Irwin; Wolchik, Sharlene; Braver, Sanford

    2009-01-01

    Most parent education programs are designed to improve child well-being following divorce by changing some aspect of parenting. However, there has been relatively little discussion of what aspects of parenting are most critical and the effectiveness of programs to change different aspects of parenting. This paper addresses these issues by: 1. Distinguishing three aspects of post-divorce parenting that have been targeted in parent education programs; 2. Reviewing evidence of the relations between each aspect of parenting and the well-being of children and; 3. Critically reviewing evidence that parent education programs have been successful in changing each aspect of post-divorce parenting. PMID:21552360

  18. Testing specificity among parents' depressive symptoms, parenting, and child internalizing and externalizing symptoms.

    Science.gov (United States)

    Gruhn, Meredith A; Dunbar, Jennifer P; Watson, Kelly H; Reising, Michelle M; McKee, Laura; Forehand, Rex; Cole, David A; Compas, Bruce E

    2016-04-01

    The present study examined the specificity in relations between observed withdrawn and intrusive parenting behaviors and children's internalizing and externalizing symptoms in an at-risk sample of children (ages 9 to 15 years old) of parents with a history of depression (N = 180). Given past findings that parental depression and parenting behaviors may differentially impact boys and girls, gender was examined as a moderator of the relations between these factors and child adjustment. Correlation and linear regression analyses showed that parental depressive symptoms were significantly related to withdrawn parenting for parents of boys and girls and to intrusive parenting for parents of boys only. When controlling for intrusive parenting, preliminary analyses demonstrated that parental depressive symptoms were significantly related to withdrawn parenting for parents of boys, and this association approached significance for parents of girls. Specificity analyses yielded that, when controlling for the other type of problem (i.e., internalizing or externalizing), withdrawn parenting specifically predicted externalizing problems but not internalizing problems in girls. No evidence of specificity was found for boys in this sample, suggesting that impaired parenting behaviors are diffusely related to both internalizing and externalizing symptoms for boys. Overall, results highlight the importance of accounting for child gender and suggest that targeting improvement in parenting behaviors and the reduction of depressive symptoms in interventions with parents with a history of depression may have potential to reduce internalizing and externalizing problems in this high-risk population. (c) 2016 APA, all rights reserved).

  19. Pilot Evaluation of a Palliative and End-of-Life Communication Intervention for Parents of Children With a Brain Tumor.

    Science.gov (United States)

    Hendricks-Ferguson, Verna L; Pradhan, Kamnesh; Shih, Chie-Schin; Gauvain, Karen M; Kane, Javier R; Liu, Jingxia; Haase, Joan E

    Providing timely palliative and end-of-life care (PC/EOL) information to parents of children with a serious illness is a national health care priority. The goals of this study were to determine feasibility, acceptability, and parent responses related to a PC/EOL communication intervention, titled "Communication Plan: Early through End of Life (COMPLETE)" to parents of children with a brain tumor. The study was a 2-site prospective, single-group pilot study targeting parents' stress and coping outcomes. The sample included 13 parents of 11 children (ie, 11 families). During the first 6 months postdiagnosis, we evaluated parent outcomes at 4 time points (baseline and 3 post-sessions). Our findings included significant decline in decision regret ( P = .0089); strong, significantly increased hope ( P ≤ .0001); and significantly decreased uncertainty ( P = .04). Over time, more than half of the parents (61.5%) preferred to receive information about their child's current condition and PC/EOL options. Our findings provide evidence to suggest that the COMPLETE intervention is feasible and acceptable and produces promising effects on 3 parent outcomes (ie, decision regret, hope, and uncertainty) in parents of children with a brain tumor. Further research is indicated to evaluate COMPLETE with a larger sample of parents of children with cancer and with a control group.

  20. Multiplex T-RFLP allows for increased target number and specificity: detection of Salmonella enterica and six species of Listeria in a single test.

    Directory of Open Access Journals (Sweden)

    Geoffrey N Elliott

    Full Text Available A multiplex T-RFLP test was developed to detect and identify Salmonella enterica and all six species of Listeria inoculated into milk at minimal levels. Extensive in silico analysis was used to design a fifteen-primer, six-amplimer methodology and in vitro application showed target organism DNA, when amplified individually, yielded the predicted terminal restriction fragments (TRFs following digestion. Non-target organisms were either not-amplified or yielded TRFs which did not interfere with target identification. Multiple target DNA analysis gave over 86% detection of total TRFs predicted, and this was improved to over 90% detection of total TRFs predicted when only two target DNA extracts were combined analysed. Co-inoculation of milk with five strains each of the target species of S. enterica and L. monocytogenes, along with five strains of the non-target species E. coli was followed by enrichment in SEL medium for M-TRFLP analysis. This allowed for detection of both target species in all samples, with detection of one S. enterica and two Listeria TRFs in all cases, and detection of a second S. enterica TRF in 91% of cases. This was from an initial inoculum of <5 cfu per 25 ml milk with a background of competing E. coli present, and gave a result from sampling of under 20 hours. The ability to increase target species number without loss of sensitivity means that extensive screening can be performed at reduced cost due to a reduction in the number of tests required.

  1. Parents' Role.

    Science.gov (United States)

    Hall, George C.

    A psychologist discusses efforts at the Boston Center for Blind Children to help parents adjust to the demands of their multiply-handicapped, visually-impaired children. The following programs are found to be helpful: an infant home visiting program (see EC 062 470)in which parents develop their role through participating in an individualized…

  2. Nasopharyngeal Pneumococcal Colonization and Impact of a Single Dose of 13-Valent Pneumococcal Conjugate Vaccine in Indian Children With HIV and Their Unvaccinated Parents.

    Science.gov (United States)

    Arya, Bikas K; Bhattacharya, Sangeeta Das; Sutcliffe, Catherine G; Ganaie, Feroze; Bhaskar, Arun; Bhattacharyya, Subhasish; Niyogi, Swapan Kumar; Moss, William J; Panda, Samiran; Ravikumar, Kadahalli Lingegowda; Das, Ranjan Saurav; Mandal, Sutapa

    2018-05-01

    Human immunodeficiency virus (HIV) infection increases risk of invasive disease from Streptococcus pneumoniae. Pneumococcal conjugate vaccines (PCV) prevent invasive disease and acquisition of vaccine type (VT) pneumococcus in the nasopharynx. To look at the safety and impact of one dose of PCV13 on acquisition of VT pneumococcal carriage in Indian children with HIV. We conducted a cohort study in families of HIV-infected children (CLH) and families of HIV-uninfected children (HUC) in West Bengal. All children received one dose of PCV13. Nasopharyngeal swabs were collected from children and parents at baseline and 2 months after vaccination. One hundred and fifteen CLH and 47 HUC received one dose of PCV13. Fifty-eight percent of CLH were on antiretroviral therapy (ART), and the median nadir CD4 count was 287. There were no significant adverse events in either group. HUC had more VT colonization than CLH-55% versus 23% of all pneumococcal isolates. HIV infection doubled the risk of nonvaccine serotype colonization (P = 0.03). There was no difference in acquisition of VT isolates in CLH (4.4%) and HUC (4.5%) post-PCV13; however, older CLH (>5 years) had decreased clearance of VT strains. ART made no difference in pneumococcal colonization at baseline or after PCV13; however, CLH with higher nadir CD4 counts before starting ART were less likely to have VT colonization post-PCV13 (prevalence ratio, 0.2; 95% confidence interval: 0.1-0.5). While there was no difference in acquisition of VT nasopharyngeal carriage of pneumococcus in CLH and HUC after one dose of PCV13, earlier access to ART may impact response to PCV13 in CLH.

  3. Parent Management

    DEFF Research Database (Denmark)

    Knudsen, Hanne

    2007-01-01

    This paper is based on a morning session at a pedagogical training course for a group of teachers at a small Danish public school. Using role-play, these teachers, under the guidance of a consultant and an actor, were practicing ‘the difficult conversation' with parents. I had been given permission...... and parents say given these assumptions? Which management responsibility is addressed through such training of the difficult conversation?  My conclusions are, briefly, that the difficult conversation is more correctly to be called an impossible conversation. It is an asking for the parent's consent...... to the teachers' description, and the teachers' authority is very easily threatened by parents who suppose that their experiences are relevant. The training situation in itself confirms that the parents are the opponents, and that the teachers should take care.The training course had been developed by the school...

  4. Parental divorce and parental death

    DEFF Research Database (Denmark)

    Marcussen, Jette; Thuen, Frode; Poul, Bruun

    2015-01-01

    The aim of this review was to identify research on children and adolescents who experience double bereavement, i.e. the experience of loss through parental divorce followed by either parental death or critical illness with imminent death. This knowledge may identify evidence to underpin knowledge...

  5. Parenting stress and parental bonding.

    Science.gov (United States)

    Willinger, Ulrike; Diendorfer-Radner, Gabriela; Willnauer, Ruth; Jörgl, Gudrun; Hager, Veronika

    2005-01-01

    Attachment experiences are thought to be important because of their implications for later development. The authors' aim with the questionnaire-based study was to investigate the differences between recalled parental bonding regarding 4 types of maternal and paternal bonding with respect to experienced parenting stress caused by child characteristics, parent attributes, and life events under the consideration of the child's gender and age. The authors gathered parental bonding behavior data with the German version of the Parental Bonding Instrument (PBI). The authors assessed parenting stress with their German version of the "Parenting Stress Index (PSI)." They found significant differences among 120 mothers grouped in the 4 maternal and the 4 paternal bonding types regarding parenting stress caused by child, maternal bonding: F(5, 113) = 4.13, p = .002, paternal bonding: F(5, 111) = 8.50, p mothers who themselves recalled the "optimal parental bonding type" with respect to the child and parental domain. The authors did not find any significant differences between the 4 maternal, F(5, 113) = 1.25, p = .29, and the 4 paternal, F(5, 111) = 1.87, p = .106, bonding types with respect to the life stress. According to the authors' findings, the representation of attachment relationships seems to have a special impact on the adult's capacity to cope with challenges and stress, either directly or indirectly as an internal working model of attachment. For the clinical practice, these findings seem to recommend the combination of both the PSI and PBI regarding the diagnostic of stressful mother-child system to plan an optimal intervention program.

  6. Engaging Urban Parents of Early Adolescents in Parenting Interventions: Home Visits vs. Group Sessions

    Science.gov (United States)

    Finigan-Carr, Nadine M.; Copeland-Linder, Nikeea; Haynie, Denise L.; Cheng, Tina L.

    2014-01-01

    Interventions targeting parents of young children have shown effectiveness, but research is lacking about best practices for engaging parents of early adolescents. Low levels of enrollment and attendance in parenting interventions present major problems for researchers and clinicians. Effective and efficient ways to engage and collaborate with…

  7. Feasibility of a School-Based Parenting Intervention for Adolescent Parents

    Science.gov (United States)

    Rispoli, Kristin M.; Sheridan, Susan M.

    2017-01-01

    Associated with complex developmental, personal, and environmental risk factors, adolescent parents have been found to display higher rates of unfavorable parenting practices than adult parents, placing their children at high risk for social, emotional and behavioral concerns. Nevertheless, interventions targeting this group often focus solely on…

  8. Teen Parents

    Science.gov (United States)

    ... Stages Listen Español Text Size Email Print Share Teen Parents Page Content Article Body A girl who ... prenatal vitamins and iron is so important. Preparing Teens For Parenthood Fears about the future are common ...

  9. Multiple Parenting

    OpenAIRE

    Dias, Paulo Cezar; Gramstrup, Erik Frederico

    2016-01-01

    With the modification of the family concept in human history, now considered the site of important value, aimed at personal fulfillment of its members, in line with the Federal Constitution of 1988 was enshrined in the principles of human dignity, affection, equal affiliations and plurality of family entities, recognizing the socio-affective parenting, founded in the state of emotional child, and biological, originally from consanguinity ties, arises multiple parenting in response to the full...

  10. High-Frequency Jet Ventilation for Complete Target Immobilization and Reduction of Planning Target Volume in Stereotactic High Single-Dose Irradiation of Stage I Non-Small Cell Lung Cancer and Lung Metastases

    International Nuclear Information System (INIS)

    Fritz, Peter; Kraus, Hans-Joerg; Muehlnickel, Werner; Sassmann, Volker; Hering, Werner; Strauch, Konstantin

    2010-01-01

    Purpose: To demonstrate the feasibility of complete target immobilization by means of high-frequency jet ventilation (HFJV); and to show that the saving of planning target volume (PTV) on the stereotactic body radiation therapy (SBRT) under HFJV, compared with SBRT with respiratory motion, can be predicted with reliable accuracy by computed tomography (CT) scans at peak inspiration phase. Methods and Materials: A comparison regarding different methods for defining the PTV was carried out in 22 patients with tumors that clearly moved with respiration. A movement span of the gross tumor volume (GTV) was defined by fusing respiration-correlated CT scans. The PTV enclosed the GTV positions with a safety margin throughout the breathing cycle. To create a PTV from CT scans acquired under HFJV, the same margins were drawn around the immobilized target. In addition, peak inspiration phase CT images (PIP-CTs) were used to approximate a target immobilized by HFJV. Results: The resulting HFJV-PTVs were between 11.6% and 45.4% smaller than the baseline values calculated as respiration-correlated CT-PTVs (median volume reduction, 25.4%). Tentative planning by means of PIP-CT PTVs predicted that in 19 of 22 patients, use of HFJV would lead to a reduction in volume of ≥20%. Using this threshold yielded a positive predictive value of 0.89, as well as a sensitivity of 0.94 and a specificity of 0.5. Conclusions: In all patients, SBRT under HFJV provided a reliable immobilization of the GTVs and achieved a reduction in PTVs, regardless of patient compliance. Tentative planning facilitated the selection of patients who could better undergo radiation in respiratory standstill, both with greater accuracy and lung protection.

  11. Random Splicing of Several Exons Caused by a Single Base Change in the Target Exon of CRISPR/Cas9 Mediated Gene Knockout

    Directory of Open Access Journals (Sweden)

    Marcel Kapahnke

    2016-12-01

    Full Text Available The clustered regularly interspaced short palindromic repeats (CRISPR-associated sequence 9 (CRISPR/Cas9 system is widely used for genome editing purposes as it facilitates an efficient knockout of a specific gene in, e.g. cultured cells. Targeted double-strand breaks are introduced to the target sequence of the guide RNAs, which activates the cellular DNA repair mechanism for non-homologous-end-joining, resulting in unprecise repair and introduction of small deletions or insertions. Due to this, sequence alterations in the coding region of the target gene frequently cause frame-shift mutations, facilitating degradation of the mRNA. We here show that such CRISPR/Cas9-mediated alterations in the target exon may also result in altered splicing of the respective pre-mRNA, most likely due to mutations of splice-regulatory sequences. Using the human FLOT-1 gene as an example, we demonstrate that such altered splicing products also give rise to aberrant protein products. These may potentially function as dominant-negative proteins and thus interfere with the interpretation of the data generated with these cell lines. Since most researchers only control the consequences of CRISPR knockout at genomic and protein level, our data should encourage to also check the alterations at the mRNA level.

  12. Teens Parenting: The Challenge of Babies and Toddlers.

    Science.gov (United States)

    Lindsay, Jeanne Warren

    This parenting guide for teenage parents of infants and toddlers incorporates numerous comments from 61 teenage parents who chose to stay in school after the birth of their child. Specific problems confronting young parents are addressed. These problems include single parenthood, early marriage, and the advantages and disadvantages of living with…

  13. A single point in protein trafficking by Plasmodium falciparum determines the expression of major antigens on the surface of infected erythrocytes targeted by human antibodies.

    Science.gov (United States)

    Chan, Jo-Anne; Howell, Katherine B; Langer, Christine; Maier, Alexander G; Hasang, Wina; Rogerson, Stephen J; Petter, Michaela; Chesson, Joanne; Stanisic, Danielle I; Duffy, Michael F; Cooke, Brian M; Siba, Peter M; Mueller, Ivo; Bull, Peter C; Marsh, Kevin; Fowkes, Freya J I; Beeson, James G

    2016-11-01

    Antibodies to blood-stage antigens of Plasmodium falciparum play a pivotal role in human immunity to malaria. During parasite development, multiple proteins are trafficked from the intracellular parasite to the surface of P. falciparum-infected erythrocytes (IEs). However, the relative importance of different proteins as targets of acquired antibodies, and key pathways involved in trafficking major antigens remain to be clearly defined. We quantified antibodies to surface antigens among children, adults, and pregnant women from different malaria-exposed regions. We quantified the importance of antigens as antibody targets using genetically engineered P. falciparum with modified surface antigen expression. Genetic deletion of the trafficking protein skeleton-binding protein-1 (SBP1), which is involved in trafficking the surface antigen PfEMP1, led to a dramatic reduction in antibody recognition of IEs and the ability of human antibodies to promote opsonic phagocytosis of IEs, a key mechanism of parasite clearance. The great majority of antibody epitopes on the IE surface were SBP1-dependent. This was demonstrated using parasite isolates with different genetic or phenotypic backgrounds, and among antibodies from children, adults, and pregnant women in different populations. Comparisons of antibody reactivity to parasite isolates with SBP1 deletion or inhibited PfEMP1 expression suggest that PfEMP1 is the dominant target of acquired human antibodies, and that other P. falciparum IE surface proteins are minor targets. These results establish SBP1 as part of a critical pathway for the trafficking of major surface antigens targeted by human immunity, and have key implications for vaccine development, and quantifying immunity in populations.

  14. Children's divorce and parent-child contact: A within-family analysis of older European parents

    NARCIS (Netherlands)

    Kalmijn, M.

    2016-01-01

    Objectives. Studies have shown that a parental divorce has a negative effect on parent-child relations. This study examines how adult children’s divorce affects the amount of contact children have with older parents, making a distinction between the effects of being single on the one hand and the

  15. Workplace Triple P: A controlled evaluation of a parenting intervention for working parents.

    Science.gov (United States)

    Sanders, Matthew R; Stallman, Helen M; McHale, Mala

    2011-08-01

    This paper examined the effects of a parenting intervention targeting working parents called Workplace Triple P. The intervention targeted both parenting and work factors, focusing on key transition times (e.g., from home to work) and trained parents to more effectively manage these transitions. One-hundred-and-twenty-one working parents with children ranging in age from 1-16 years were randomly assigned to either a Workplace Triple P condition (WPTP) or to a waitlist control condition (WLC). Results showed that parents who had received the intervention reported significantly lower levels on measures of personal distress and dysfunctional parenting; and higher levels of work commitment, work satisfaction, and self-efficacy. Implications for the delivery of parenting interventions as employee assistance programs are discussed along with how such interventions can enhance work and family life.

  16. Parental Marital Quality, Parental Divorce, and Relations with Parents.

    Science.gov (United States)

    Booth, Alan; Amato, Paul R.

    1994-01-01

    Examined data from 419 parents and their adult children to assess impact of parental marital quality and divorce while child is residing with parents on parent-child relations 12 years later. Low marital quality and divorce appeared to have independent effects on adult child-parent relations. Fathers' relationships suffered more than mothers';…

  17. Impact of Caregiving for a Child With Cancer on Parental Health Behaviors, Relationship Quality, and Spiritual Faith: Do Lone Parents Fare Worse?

    OpenAIRE

    Wiener, Lori; Viola, Adrienne; Kearney, Julia; Mullins, Larry L.; Sherman-Bien, Sandra; Zadeh, Sima; Farkas-Patenaude, Andrea; Pao, Maryland

    2015-01-01

    Caregiving stress has been associated with changes in the psychological and physical health of parents of children with cancer, including both partnered and single parents. While parents who indicate “single” on a demographic checklist are typically designated as single parents, a parent can be legally single and still have considerable support caring for an ill child. Correspondingly, an individual can be married/partnered and feel alone when caring for a child with serious illness. In the c...

  18. Long-Gradient Separations Coupled with Selected Reaction Monitoring for Highly Sensitive, Large Scale Targeted Protein Quantification in a Single Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Shi, Tujin; Fillmore, Thomas L.; Gao, Yuqian; Zhao, Rui; He, Jintang; Schepmoes, Athena A.; Nicora, Carrie D.; Wu, Chaochao; Chambers, Justin L.; Moore, Ronald J.; Kagan, Jacob; Srivastava, Sudhir; Liu, Alvin Y.; Rodland, Karin D.; Liu, Tao; Camp, David G.; Smith, Richard D.; Qian, Weijun

    2013-10-01

    Long-gradient separations coupled to tandem MS were recently demonstrated to provide a deep proteome coverage for global proteomics; however, such long-gradient separations have not been explored for targeted proteomics. Herein, we investigate the potential performance of the long-gradient separations coupled with selected reaction monitoring (LG-SRM) for targeted protein quantification. Direct comparison of LG-SRM (5 h gradient) and conventional LC-SRM (45 min gradient) showed that the long-gradient separations significantly reduced background interference levels and provided an 8- to 100-fold improvement in LOQ for target proteins in human female serum. Based on at least one surrogate peptide per protein, an LOQ of 10 ng/mL was achieved for the two spiked proteins in non-depleted human serum. The LG-SRM detection of seven out of eight endogenous plasma proteins expressed at ng/mL or sub-ng/mL levels in clinical patient sera was also demonstrated. A correlation coefficient of >0.99 was observed for the results of LG-SRM and ELISA measurements for prostate-specific antigen (PSA) in selected patient sera. Further enhancement of LG-SRM sensitivity was achieved by applying front-end IgY14 immunoaffinity depletion. Besides improved sensitivity, LG-SRM offers at least 3 times higher multiplexing capacity than conventional LC-SRM due to ~3-fold increase in average peak widths for a 300-min gradient compared to a 45-min gradient. Therefore, LG-SRM holds great potential for bridging the gap between global and targeted proteomics due to its advantages in both sensitivity and multiplexing capacity.

  19. Single-Parent and Working-Parent Heart Health

    Science.gov (United States)

    ... Search By Zipcode Search by State SELECT YOUR LANGUAGE Español (Spanish) 简体中文 (Traditional Chinese) 繁体中文 (Simplified Chinese) ... Hey Kids, Learn About Blood Sugar and Diabetes Teaching Gardens Teaching Gardens Recognition Teaching Gardens-See Our ...

  20. Identification of singles bar as a direct transcriptional target of Drosophila Myocyte enhancer factor-2 and a regulator of adult myoblast fusion

    OpenAIRE

    Brunetti, Tonya M.; Fremin, Brayon J.; Cripps, Richard M.

    2015-01-01

    In Drosophila, myoblast fusion is a conserved process in which founder cells (FCs) and fusion competent myoblasts (FCMs) fuse to form a syncytial muscle fiber. Mutants for the myogenic regulator Myocyte enhancer factor-2 (MEF2) show a failure of myoblast fusion, indicating that MEF2 regulates the fusion process. Indeed, chromatin immunoprecipitation studies show that several genes involved in myoblast fusion are bound by MEF2 during embryogenesis. Of these, the MARVEL domain gene singles bar ...

  1. Parental influences on sperm banking attempts among adolescent males newly diagnosed with cancer.

    Science.gov (United States)

    Klosky, James L; Flynn, Jessica S; Lehmann, Vicky; Russell, Kathryn M; Wang, Fang; Hardin, Robin N; Eddinger, Jasmine R; Zhang, Hui; Schenck, Lauren A-M; Schover, Leslie R

    2017-12-01

    To investigate the influence of parental sociodemographic, communication, and psychological factors on sperm collection attempts among at-risk adolescent males newly diagnosed with cancer. Prospective, single group, observational study design. Pediatric oncology centers. Parents (N = 144) of 122 newly diagnosed adolescent males at increased risk for infertility secondary to cancer therapy. Survey-based assessment of parent factors associated with adolescent collection attempts. Attempt of manual collection of sperm. Parental recommendation to bank sperm (odds ratio [OR] 3.72; 95% confidence interval [CI] 1.18-11.76) and perceived self-efficacy to facilitate banking (OR 1.20; 95% CI 1.02-1.41) were associated with an increased likelihood of making a collection attempt. Parental recommendation to bank is a critical influence for sperm banking among adolescent males newly diagnosed with cancer. These findings highlight the importance of effective communication between parents, patients, and health-care teams when discussing preservation options. Parent perceptions of their ability to facilitate sperm banking at the time of diagnosis should also be targeted in future interventions. NCT01152268. Copyright © 2017. Published by Elsevier Inc.

  2. Single-spin asymmetry in electro-production of {pi}{sup +} {pi}{sup -} pairs from a transversely polarized proton target at the HERMES experiment

    Energy Technology Data Exchange (ETDEWEB)

    Lu, Xiao-Rui

    2008-10-15

    In this thesis, the measurement of an azimuthal amplitude of the asymmetry in the lepto-production of {pi}{sup +}{pi}{sup -} pairs at the HERMES experiment is reported. The experiment was carried out at DESY in Germany, utilizing the longitudinally polarized 27.6 GeV electron/positron beam of the HERA storage ring in combination with a longitudinally or transversely polarized gaseous target internal to the beam pipe. For the present measurement, the transversely polarized proton target was used and the beam polarization was averaged out in order to measure the asymmetry A{sub UT}. A Ring Imaging Cerenkov (RICH) detector allows the precise identification of pions, kaons and protons over essentially the entire momentum range of the experiment. The asymmetry A{sub UT} for {pi}{sup +}{pi}{sup -} pair production was measured for the first time in the world by HERMES. The amplitudes are extracted as functions of different kinematic variables, which can facilitate the comparison with the theoretical models and the extraction of transversity with combination of the measurement of the dihadron fragmentation function. (orig.)

  3. Parental Power and Adolescents' Parental Identification.

    Science.gov (United States)

    Acock, Alan C.; Yang, Wen Shan

    1984-01-01

    Combines McDonald's social power of parental identification with sex-linked models of parental identification to account for the identification of daughters (N=199) and sons (N=147) with their parents. Found that because of a halo effect, a gain in identification with one parent is not at the other parent's expense. (JAC)

  4. What are other parents saying? Perceived parental communication norms and the relationship between alcohol-specific parental communication and college student drinking.

    Science.gov (United States)

    Napper, Lucy E; Hummer, Justin F; Lac, Andrew; Labrie, Joseph W

    2014-03-01

    This study examined parents' normative perceptions of other college parents' alcohol-specific communication, and how parents' perceived communication norms and alcohol-specific communication relate to student drinking outcomes. A sample of 457 student-parent dyads were recruited from a midsize university. Students completed Web-based assessments of alcohol-related attitudes and behaviors. Parents completed alcohol-specific measures of communication norms and parent-child communication, including communication content (i.e., targeted communication) and frequency of communication. Results indicated that parents overestimated how much other parents talked to their college students about the frequency and quantity of alcohol use, but underestimated how often parents initiated conversations about alcohol. In a path model, perceived communication norms positively predicted both targeted communication and frequency of communication. Perceived communication norms and targeted communication negatively predicted students' attitude toward alcohol use. In contrast, more frequent communication predicted students holding more approving attitudes toward alcohol. The relationship between parents' perceived communication norms and students' drinking behaviors was mediated by the parental communication variables and student attitudes. Tests of indirect effects were undertaken to examine meditational processes. The findings underscore relations involving parental perceived communication norms and parents' own alcohol communication and their children's drinking outcomes. The complex relationships of different types of parental communication and student outcomes warrant further research.

  5. Study protocol for Enhancing Parenting In Cancer (EPIC): development and evaluation of a brief psycho-educational intervention to support parents with cancer who have young children.

    Science.gov (United States)

    Stafford, Lesley; Sinclair, Michelle; Turner, Jane; Newman, Louise; Wakefield, Claire; Krishnasamy, Mei; Mann, G Bruce; Gilham, Leslie; Mason, Kylie; Rauch, Paula; Cannell, Julia; Schofield, Penelope

    2017-01-01

    Parents with cancer have high rates of psychological morbidity, and their children are at risk of poor psychosocial outcomes, particularly in the context of parental distress and poor family communication. Parents express concerns about the impact of cancer on their children and report a lack of professional guidance in meeting their children's needs. Few parenting interventions exist and current interventions have extensive infrastructure demands making them unsuitable for routine use in most health settings. The aims of this study are to develop and establish the feasibility and acceptability of a novel and accessible psycho-educational intervention to improve parenting efficacy and decrease parental stress among adults with cancer who have children aged 3-12 years. The intervention will be suitable for parents with cancer who are receiving treatment with a view to longer term survival, irrespective of cancer diagnosis, and their respective co-parents. This study comprises two phases using the UK Medical Research Council framework for developing complex interventions. In the development phase, intervention content will be iteratively developed and evaluated in consultation with consumers, and in the piloting phase, feasibility will be tested in a clinical sample of 20 parents with cancer and their co-parents using a single arm, pre-test post-test design. The intervention will comprise an audiovisual resource (DVD), a question prompt list, and a telephone call with a clinical psychologist. Questionnaires administered pre- and 1 month post-intervention will assess parental stress, psychological morbidity, quality of life, self-efficacy and perceptions of child adjustment, and family functioning. Intervention feasibility will be determined by mixed-method participant evaluation of perceived usefulness, benefits, and acceptability. This new initiative will translate existing descriptive evidence into an accessible intervention that supports parenting during cancer

  6. Single-dose safety and pharmacokinetic evaluation of fluorocoxib A: pilot study of novel cyclooxygenase-2-targeted optical imaging agent in a canine model.

    Science.gov (United States)

    Cekanova, Maria; Uddin, Md Jashim; Legendre, Alfred M; Galyon, Gina; Bartges, Joseph W; Callens, Amanda; Martin-Jimenez, Tomas; Marnett, Lawrence J

    2012-11-01

    We evaluated preclinical single-dose safety, pharmacokinetic properties, and specific uptake of the new optical imaging agent fluorocoxib A in dogs. Fluorocoxib A, N-[(5-carboxy-X-rhodaminyl)but-4-yl]-2-[1-(4-chlorobenzoyl)-5-methoxy-2-methyl-1H-indol-3-yl]acetamide, selectively binds and inhibits the cyclooxygenase-2 (COX-2) enzyme, which is overexpressed in many cancers. Safety pilot studies were performed in research dogs following intravenous (i.v.) administration of 0.1 and 1  mg/kg fluorocoxib A. Blood and urine samples collected three days after administration of each dose of fluorocoxib A revealed no evidence of toxicity, and no clinically relevant adverse events were noted on physical examination of exposed dogs over that time period. Pharmacokinetic parameters were assessed in additional research dogs from plasma collected at several time points after i.v. administration of fluorocoxib A using high-performance liquid chromatography analysis. The pharmacokinetic studies using 1  mg/kg showed a peak of fluorocoxib A (92±28  ng/ml) in plasma collected at 0.5 h. Tumor specific uptake of fluorocoxib A was demonstrated using a dog diagnosed with colorectal cancer expressing COX-2. Our data support the safe single-dose administration and in vivo efficacy of fluorocoxib A, suggesting a high potential for successful translation to clinical use as an imaging agent for improved tumor detection in humans.

  7. Single parent families: diversity, myths and realities

    National Research Council Canada - National Science Library

    Hanson, Shirley M. H

    1995-01-01

    ...: * * * . * * * * * * * * * * * * * * * * * * * * Cults and the Family, edited by Florence Kaslow and Marvin B. Sussman Alternatives to Traditional Family Living, edited by Harriet Gross and Marvin B. Sussman...

  8. Parental involvement in interventions to improve child diet and prevent disease

    Science.gov (United States)

    Parents influence children's dietary intake in part through general parenting styles, feeding styles, and/or food parenting practices. Interventions aimed at improving child diet often include parent components. A systematic review was conducted to assess the effect of targeting parenting styles and...

  9. Beyond nearly zero-energy buildings: Experimental investigation of the thermal indoor environment and energy performance of a single-family house designed for plus-energy targets

    DEFF Research Database (Denmark)

    Kazanci, Ongun Berk; Olesen, Bjarne W.

    2016-01-01

    A detached, one-story, single-family house in Denmark was operated with different heating and cooling strategies for 1 year. The strategies compared during the heating season were floor heating without ventilation, floor heating supplemented by warm air heating (ventilation system), and floor...... heating with heat recovery from exhaust air. During the cooling season, the house was cooled by floor cooling and was ventilated mechanically. Air and globe (operative, when applicable) temperatures at different heights at a central location were recorded. The thermal indoor environment, local thermal...... discomfort and overheating were evaluated based on EN 15251 (2007), EN ISO 7730 (2005), and DS 469 (2013), respectively. Energy performance was evaluated based on the energy production and HVAC system energy use. The thermal indoor environment during the heating season was satisfactory...

  10. The Relation between Family Structure and Young Adolescents' Appraisals of Family Climate and Parenting Behavior.

    Science.gov (United States)

    Kurdek, Lawrence A.; Fine, Mark A.

    1993-01-01

    Young adolescents who lived with both biological parents, single divorced mother, single divorced father, mother and stepfather, father and stepmother, or multiply divorced parent appraised dimensions of family climate and dimensions of parenting. Differences among family structures were found on warmth, conflict, permissive parenting, and…

  11. Single cell imprinting on the surface of Ag-ZnO bimetallic nanoparticle modified graphene oxide sheets for targeted detection, removal and photothermal killing of E. Coli.

    Science.gov (United States)

    Roy, Ekta; Patra, Santanu; Tiwari, Ashutosh; Madhuri, Rashmi; Sharma, Prashant K

    2017-03-15

    A very cost-effective, fast, sensitive and specific imprinted polymer modified electrochemical sensor for the targeted detection, removal and destruction of Escherichia coli bacteria was developed onto the surface of Ag-ZnO bimetallic nanoparticle and graphene oxide nanocomposite. The nanocomposite played a dual role in this work, as a platform for imprinting of bacteria as well as a participated in their laser-light induced photo killing. In terms of sensing, our proposed sensor can detect E. Coli as few as 10CFUmL -1 and capture 98% of bacterial cells from their very high concentrated solution (10 5 CFUmL -1 ). Similarly to the quantitative detection, we have also investigated the quantitative destruction of E. Coli and found that 16.0cm 2 area of polymer modified glass plate is sufficient enough to kill 10 5 CFUmL -1 in the small time span of 5 minutes. The obtained results suggest that our proposed sensor have potential to serve as a promising candidate for specific and quantitative detection, removal as well as the destruction of a variety of bacterial pathogens. Copyright © 2015 Elsevier B.V. All rights reserved.

  12. Parent and Child Agreement for Acute Stress Disorder, Post-Traumatic Stress Disorder and Other Psychopathology in a Prospective Study of Children and Adolescents Exposed to Single-Event Trauma

    Science.gov (United States)

    Meiser-Stedman, Richard; Smith, Patrick; Glucksman, Edward; Yule, William; Dalgleish, Tim

    2007-01-01

    Examining parent-child agreement for Acute Stress Disorder (ASD) and Post-Traumatic Stress Disorder (PTSD) in children and adolescents is essential for informing the assessment of trauma-exposed children, yet no studies have examined this relationship using appropriate statistical techniques. Parent-child agreement for these disorders was examined…

  13. Adapting to the global shortage of cholera vaccines: targeted single dose cholera vaccine in response to an outbreak in South Sudan.

    Science.gov (United States)

    Parker, Lucy A; Rumunu, John; Jamet, Christine; Kenyi, Yona; Lino, Richard Laku; Wamala, Joseph F; Mpairwe, Allan M; Ciglenecki, Iza; Luquero, Francisco J; Azman, Andrew S; Cabrol, Jean-Clement

    2017-04-01

    Shortages of vaccines for epidemic diseases, such as cholera, meningitis, and yellow fever, have become common over the past decade, hampering efforts to control outbreaks through mass reactive vaccination campaigns. Additionally, various epidemiological, political, and logistical challenges, which are poorly documented in the literature, often lead to delays in reactive campaigns, ultimately reducing the effect of vaccination. In June 2015, a cholera outbreak occurred in Juba, South Sudan, and because of the global shortage of oral cholera vaccine, authorities were unable to secure sufficient doses to vaccinate the entire at-risk population-approximately 1 million people. In this Personal View, we document the first public health use of a reduced, single-dose regimen of oral cholera vaccine, and show the details of the decision-making process and timeline. We also make recommendations to help improve reactive vaccination campaigns against cholera, and discuss the importance of new and flexible context-specific dose regimens and vaccination strategies. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. A Single CRISPR-Cas9 Deletion Strategy that Targets the Majority of DMD Patients Restores Dystrophin Function in hiPSC-Derived Muscle Cells.

    Science.gov (United States)

    Young, Courtney S; Hicks, Michael R; Ermolova, Natalia V; Nakano, Haruko; Jan, Majib; Younesi, Shahab; Karumbayaram, Saravanan; Kumagai-Cresse, Chino; Wang, Derek; Zack, Jerome A; Kohn, Donald B; Nakano, Atsushi; Nelson, Stanley F; Miceli, M Carrie; Spencer, Melissa J; Pyle, April D

    2016-04-07

    Mutations in DMD disrupt the reading frame, prevent dystrophin translation, and cause Duchenne muscular dystrophy (DMD). Here we describe a CRISPR/Cas9 platform applicable to 60% of DMD patient mutations. We applied the platform to DMD-derived hiPSCs where successful deletion and non-homologous end joining of up to 725 kb reframed the DMD gene. This is the largest CRISPR/Cas9-mediated deletion shown to date in DMD. Use of hiPSCs allowed evaluation of dystrophin in disease-relevant cell types. Cardiomyocytes and skeletal muscle myotubes derived from reframed hiPSC clonal lines had restored dystrophin protein. The internally deleted dystrophin was functional as demonstrated by improved membrane integrity and restoration of the dystrophin glycoprotein complex in vitro and in vivo. Furthermore, miR31 was reduced upon reframing, similar to observations in Becker muscular dystrophy. This work demonstrates the feasibility of using a single CRISPR pair to correct the reading frame for the majority of DMD patients. Copyright © 2016 Elsevier Inc. All rights reserved.

  15. Concurrent predictors of dysfunctional parenting and maternal confidence: implications for parenting interventions.

    Science.gov (United States)

    Morawska, A; Sanders, M R

    2007-11-01

    The often intense nature of the conflict between parents and their toddlers requires better understanding of what happens during this stage of development and how difficulties can be prevented from escalating in the future. Clarification of the nature of family and parenting factors related to toddler behaviour allows better capacity for intervention development and tailoring to individual families. A total of 126 mothers of toddlers completed a self-report assessment battery, examining child behaviour, parenting style and confidence, as well as broader family adjustment measures. The study found that maternal confidence and dysfunctional parenting were interrelated and were also predicted best by parenting variables, in contrast to socio-demographic and child variables. Maternal confidence also mediated the relationships between family income and toddler behaviour. Parenting style and confidence are important modifiable factors to target in parenting interventions. The implications for the development, implementation and delivery of parenting interventions are discussed.

  16. Syntrophic interactions and mechanisms underpinning anaerobic methane oxidation: targeted metaproteogenomics, single-cell protein detection and quantitative isotope imaging of microbial consortia

    Energy Technology Data Exchange (ETDEWEB)

    Orphan, Victoria Jeanne [California Inst. of Technology (CalTech), Pasadena, CA (United States). Division of Geological and Planetary Sciences

    2014-11-26

    Syntrophy and mutualism play a central role in carbon and nutrient cycling by microorganisms. Yet, our ability to effectively study symbionts in culture has been hindered by the inherent interdependence of syntrophic associations, their dynamic behavior, and their frequent existence at thermodynamic limits. Now solutions to these challenges are emerging in the form of new methodologies. Developing strategies that establish links between the identity of microorganisms and their metabolic potential, as well as techniques that can probe metabolic networks on a scale that captures individual molecule exchange and processing, is at the forefront of microbial ecology. Understanding the interactions between microorganisms on this level, at a resolution previously intractable, will lead to our greater understanding of carbon turnover and microbial community resilience to environmental perturbations. In this project, we studied an enigmatic syntrophic association between uncultured methane-oxidizing archaea and sulfate-reducing bacteria. This environmental archaeal-bacterial partnership represents a globally important sink for methane in anoxic environments. The specific goals of this project were organized into 3 major tasks designed to address questions relating to the ecophysiology of these syntrophic organisms under changing environmental conditions (e.g. different electron acceptors and nutrients), primarily through the development of microanalytical imaging methods which enable the visualization of the spatial distribution of the partners within aggregates, consumption and exchange of isotopically labeled substrates, and expression of targeted proteins identified via metaproteomics. The advanced tool set developed here to collect, correlate, and analyze these high resolution image and isotope-based datasets from methane-oxidizing consortia has the potential to be widely applicable for studying and modeling patterns of activity and interactions across a broad range of

  17. Teenage parents and their offspring.

    Science.gov (United States)

    Kaufman, J

    1996-06-18

    Teenage parents are cast into adult roles before the role experimentation and identity development tasks of middle adolescence can be completed. Understanding the etiology of this social problem requires an ecological perspective encompassing individual characteristics, person-context variables, and societal factors such as race and social class. Risk factors identified in the literature on adolescent pregnancy in the US include: absence of a future orientation or aspirations, lack of assertiveness and interpersonal skills to control physical intimacy, low socioeconomic status and minority group membership, growing up in a single-parent family, a history of sexual abuse, five or more siblings, a sister or friend who became a teenage mother, lax parental supervision of dating and free time, low self-esteem, and dropping out or failing in school. The limited data on adolescent fathers suggest they have histories of substance use, delinquency, failure to graduate from high school, financial difficulty, and exposure to family violence. The offspring of adolescent parents show a higher incidence of developmental delays and mild mental retardation than children of adults and are at increased risk of child abuse and neglect. Teen parents raised in dysfunctional families tend to perpetuate destructive methods of child rearing and have unrealistic, age-inappropriate expectations for infants and toddlers. Teenage parents' lack of competence can be mitigated, however, by positive living arrangements, a supportive family of origin, peer support groups, quality child care, school-based services, and accurate information about parenting and child development.

  18. Measurement of the Target-Normal Single-Spin Asymmetry Ayn in the Deep Inelastic Region from the Reaction 3He(e,e')

    Energy Technology Data Exchange (ETDEWEB)

    Katich, Joseph [Coll. of William and Mary, Williamsburg, VA (United States)

    2011-01-01

    A first measurement of the inclusive target single-spin asymmetry, Any, has been performed in deep-inelastic scattering of electrons from a 3He target polarized normal to the electron scattering plane. This asymmetry is void of contributions at the Born level, and thus is a direct observable for two-photon physics. The experiment was performed in Hall A at Thomas Jefferson National Accelerator Facility from October 2008 through early February 2009. The measurement is the first from a polarized neutron target. The final overall precision is several times better than previously existing SLAC proton data, and significantly extends the kinematic range over which the asymmetry has been measured. The asymmetry was measured at five kinematic points in the deep inelastic scattering region covering Q2 = 1 - 3 GeV2 and xB = 0.16 to 0.41. The asymmetry varied from 0.006 to 0.071 with astatistical precision at the 10-2 level.

  19. Single-stranded DNA aptamer targeting and neutralization of anti-D alloantibody: a potential therapeutic strategy for haemolytic diseases caused by Rhesus alloantibody.

    Science.gov (United States)

    Zhang, Yinze; Wu, Fan; Wang, Manni; Zhuang, Naibao; Zhou, Huayou; Xu, Hua

    2018-02-01

    Rhesus (Rh) D antigen is the most important antigen in the Rh blood group system because of its strong immunogenicity. When RhD-negative individuals are exposed to RhD-positive blood, they may produce anti-D alloantibody, potentially resulting in delayed haemolytic transfusion reactions and Rh haemolytic disease of the foetus and newborn, which are difficult to treat. Inhibition of the binding of anti-D antibody with RhD antigens on the surface of red blood cells may effectively prevent immune haemolytic diseases. In this study, single-stranded (ss) DNA aptamers, specifically binding to anti-D antibodies, were selected via systematic evolution of ligands by exponential enrichment (SELEX) technology. After 14 rounds of selection, the purified ssDNA was sequenced using a Personal Genome Machine system. Haemagglutination inhibition assays were performed to screen aptamers for biological activity in terms of blocking antigen-antibody reactions: the affinity and specificity of the aptamers were also determined. In addition to high specificity, the aptamers which were selected showed high affinity for anti-D antibodies with dissociation constant (K d ) values ranging from 51.46±14.90 to 543.30±92.59 nM. By the combined use of specific ssDNA aptamer 7 and auxiliary ssDNA aptamer 2, anti-D could be effectively neutralised at low concentrations of the aptamers. Our results demonstrate that ssDNA aptamers may be a novel, promising strategy for the treatment of delayed haemolytic transfusion reactions and Rh haemolytic disease of the foetus and newborn.

  20. Adolescents? Behaviors as Moderators for the Link between Parental Self-Efficacy and Parenting Practices

    OpenAIRE

    Glatz, Terese; Cotter, Allison; Buchanan, Christy M.

    2016-01-01

    Based on theory that parents with higher levels of self-efficacy (PSE) should find it easier to parent effectively in the face of challenging child behaviors than should parents with lower levels of PSE, this study examines the link between PSE and parenting using children?s behaviors as potential moderators. Participants were 130 parents who had an older adolescent (M age?=?17.58) in addition to the target adolescent (M age?=?11.79), and both adolescents? externalizing behaviors were used as...

  1. Effects of the Practiced Routines Parent Training Program on Behavioral Strategy Use, Parental Well-Being, and Child Challenging Behavior in Parents of Children with Autism Spectrum Disorder

    Science.gov (United States)

    Raulston, Tracy Jane

    2017-01-01

    In this study, a concurrent randomized multiple baseline across three parent-child dyads single-case design was employed to evaluate the effects of a brief three-week parent training program, titled Practiced Routines. The Practiced Routines parent training program included positive behavior supports (PBS) and mindfulness strategies within the…

  2. Parental care influences leukocyte telomere length with gender specificity in parents and offsprings.

    Science.gov (United States)

    Enokido, Masanori; Suzuki, Akihito; Sadahiro, Ryoichi; Matsumoto, Yoshihiko; Kuwahata, Fumikazu; Takahashi, Nana; Goto, Kaoru; Otani, Koichi

    2014-10-03

    There have been several reports suggesting that adverse childhood experiences such as physical maltreatment and long institutionalization influence telomere length. However, there has been no study examining the relationship of telomere length with variations in parental rearing. In the present study, we examined the relationship of leukocyte telomere length with parental rearing in healthy subjects. The subjects were 581 unrelated healthy Japanese subjects. Perceived parental rearing was assessed by the Parental Bonding Instrument consisting of the care and protection factors. Leukocyte relative telomere length was determined by a quantitative real-time PCR method for a ratio of telomere/single copy gene. In the multiple regression analyses, shorter telomere length in males was related to lower scores of paternal care (β = 0.139, p parental care and telomere length which covers both lower and higher ends of parental care, and that the effects of parental care on telomere length are gender-specific in parents and offsprings.

  3. Do incest, depression, parental drinking, serious romantic relationships, and living with parents influence patterns of substance use during emerging adulthood?

    Science.gov (United States)

    Snyder, Susan M; Rubenstein, Casey

    2014-01-01

    This study examined how incest, depression, parental drinking, relationship status, and living with parents affect patterns of substance use among emerging adults, 18 to 25 years old. The study sample included (n = 11,546) individuals who participated in Waves I, II, and III of the National Longitudinal Study of Adolescent Health (Add Health). The study used separate latent class analysis for males and females to determine how patterns of substance use clustered together. The study identified the following three classes of substance use: heavy, moderate, and normative substance use patterns. Multinomial logistic regression indicated that, for females only, incest histories also nearly doubled the risk of heavy-use class membership. In addition, experiencing depression, being single, and not living with parents serve as risk factors for males and females in the heavy-use group. Conversely, being Black, Hispanic, or living with parents lowered the likelihood of being in the group with the most substance use behaviors (i.e., heavy use). Findings highlight the need for interventions that target depression and female survivors of incest among emerging adults.

  4. Parental Identity and Its Relation to Parenting and Psychological Functioning in Middle Age.

    Science.gov (United States)

    Fadjukoff, Päivi; Pulkkinen, Lea; Lyyra, Anna-Liisa; Kokko, Katja

    2016-04-02

    Objective. This article focuses on identity as a parent in relation to parenting and psychological functioning in middle age. Design. Drawn from the Jyväskylä Longitudinal Study of Personality and Social Development, 162 participants (53% females) with children (age 36), represented the Finnish age-cohort born in 1959. Parental identity was assessed at ages 36, 42, and 50. Results. In both women and men, parental identity achievement increased from age 36 to 42 and remained stable to 50. The level of parental identity achievement was higher in women than in men. Achievement was typical for women and foreclosure for men. Participants' education, occupational status, and number of offspring were not related to parental identity status. As expected, parental identity achievement was associated with authoritative (indicated by higher nurturance and parental knowledge about the child's activities) parenting style. No significant associations emerged between parental identity foreclosure and restrictiveness as an indicator of authoritarian parenting style. The diffused men outscored others in parental stress. Achieved parental identity was related to generativity in both genders and to higher psychological and social well-being in men. Conclusions. At present, many parenting programs are targeted to young parents. This study highlighted the importance of a later parenting phase at around age 40, when for many, the children are approaching puberty. Therefore, parenting programs and support should also be designed for middle-aged parents. Specifically men may need additional support for their active consideration and engagement in the fathering role. © Päivi Fadjukoff, Lea Pulkkinen, Anna-Liisa Lyyra, and Katja Kokko This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in

  5. Parental Identity and Its Relation to Parenting and Psychological Functioning in Middle Age

    Science.gov (United States)

    Fadjukoff, Päivi; Pulkkinen, Lea; Lyyra, Anna-Liisa; Kokko, Katja

    2016-01-01

    SYNOPSIS Objective. This article focuses on identity as a parent in relation to parenting and psychological functioning in middle age. Design. Drawn from the Jyväskylä Longitudinal Study of Personality and Social Development, 162 participants (53% females) with children (age 36), represented the Finnish age-cohort born in 1959. Parental identity was assessed at ages 36, 42, and 50. Results. In both women and men, parental identity achievement increased from age 36 to 42 and remained stable to 50. The level of parental identity achievement was higher in women than in men. Achievement was typical for women and foreclosure for men. Participants’ education, occupational status, and number of offspring were not related to parental identity status. As expected, parental identity achievement was associated with authoritative (indicated by higher nurturance and parental knowledge about the child’s activities) parenting style. No significant associations emerged between parental identity foreclosure and restrictiveness as an indicator of authoritarian parenting style. The diffused men outscored others in parental stress. Achieved parental identity was related to generativity in both genders and to higher psychological and social well-being in men. Conclusions. At present, many parenting programs are targeted to young parents. This study highlighted the importance of a later parenting phase at around age 40, when for many, the children are approaching puberty. Therefore, parenting programs and support should also be designed for middle-aged parents. Specifically men may need additional support for their active consideration and engagement in the fathering role. © Päivi Fadjukoff, Lea Pulkkinen, Anna-Liisa Lyyra, and Katja Kokko This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and

  6. Parenting Styles and Beliefs about Parental Authority.

    Science.gov (United States)

    Smetana, Judith G.

    1994-01-01

    Suggests that models of parenting style, such as Baumrind's popular model, are insensitive to variations in parenting resulting from characteristics of the different situations in which the parenting is expressed. Argues that considering parenting in context adds greater specificity to the model and enhances the potential for predicting child…

  7. ROS-Responsive Mitochondria-Targeting Blended Nanoparticles: Chemo- and Photodynamic Synergistic Therapy for Lung Cancer with On-Demand Drug Release upon Irradiation with a Single Light Source

    Science.gov (United States)

    Yue, Caixia; Yang, Yuming; Zhang, Chunlei; Alfranca, Gabriel; Cheng, Shangli; Ma, Lijun; Liu, Yanlei; Zhi, Xiao; Ni, Jian; Jiang, Weihua; Song, Jie; de la Fuente, Jesús M.; Cui, Daxiang

    2016-01-01

    Mitochondria in cancer cells maintain a more negative membrane potential than normal cells. Mitochondria are the primary source of cellular reactive oxygen species (ROS), which are necessary for photodynamic therapy. Thus, the strategy of targeting mitochondria can maximize the photodynamic therapeutic efficiency for cancer. Here we report, for the first time, synthesis of a new mitochondria-targeting drug delivery system, ZnPc/CPT-TPPNPs. To synthesize this novel compound, polyethylene glycol was functionalized with thioketal linker-modified camptothecin (TL-CPT) and triphenylphosphonium to form the block copolymer, TL-CPT-PEG1K-TPP. The ZnPc/CPT-TPPNPs was constructed for delivery of the photosensitizer Zinc phthalocyanine (ZnPc) by blending the block copolymer TL-CPT-PEG1K-TPP with 1, 2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)] (DSPE-PEG).Triphenylphosphine can accumulate selectively several hundred-fold within mitochondria. The thioketal linker is ROS-responsive and CPT can be released upon ROS cleavage. We also show that the ZnPc loaded in ZnPc/CPT-TPPNPs absorbed the 633 nm laser to produce ROS, which could be utilized both in photodynamic therapy and to cleave the thioketal linker thereby releasing camptothecin for chemotherapy. Thus, the mitochondria-targeting nanoparticles could elevate photodynamic therapeutic efficacy. Our results showed that surface modification of the nanoparticles with triphenylphosphine cations facilitated efficient subcellular delivery of the photosensitizer to mitochondria. The nanoparticles had a good ROS-responsive effect to release CPT, which could transfer to the nucleus and interfere with DNA replication as a topoisomeraseⅠinhibitor. Thus, the blended nanoparticles provide a new promising approach as a mitochondria-targeting ROS-activated chemo- and photodynamic therapy with a single light source for lung cancer. PMID:27877240

  8. Targetting Teenagers.

    Science.gov (United States)

    Bernard van Leer Foundation Newsletter, 1995

    1995-01-01

    This theme issue of the Bernard van Leer Foundation newsletter focuses on the problem of teenage pregnancy and teenage parenthood in developing and developed nations, and examines the problems that teenage mothers face in different societies. It explores societal norms and values related to teenage parenting; the effects of teenage parents on…

  9. Parental Preferences for the Organization of Preschool Vaccination Programs Including Financial Incentives: A Discrete Choice Experiment

    Directory of Open Access Journals (Sweden)

    Darren Flynn PhD

    2017-05-01

    Full Text Available Objective: To establish preferences of parents and guardians of preschool children for the organization of preschool vaccination services, including financial incentives. Design: An online discrete choice experiment. Participants: Parents and guardians of preschool children (up to age 5 years who were (n = 259 and were not (n = 262 classified as at high risk of incompletely vaccinating their children. High risk of incomplete vaccination was defined as any of the following: aged less than 20 years, single parents, living in one of the 20% most deprived areas in England, had a preschool child with a disability, or had more than three children. Main Outcome Measures: Participant preferences expressed as positive (utility or negative (disutility on eight attributes and levels describing the organization of preschool vaccination programs. Results: There was no difference in preference for parental financial incentives compared to no incentive in parents “not at high risk” of incomplete vaccination. Parents who were “at high risk” expressed utility for cash incentives. Parents “at high risk” of incomplete vaccination expressed utility for information on the risks and benefits of vaccinations to be provided as numbers rather than charts or pictures. Both groups preferred universally available, rather than targeted, incentives. Utility was identified for shorter waiting times, and there were variable preferences for who delivered vaccinations. Conclusions: Cash incentives for preschool vaccinations in England would be welcomed by parents who are “at high risk” of incompletely vaccinating their children. Further work is required on the optimal mode and form of presenting probabilistic information on vaccination to parents/guardians, including preferences on mandatory vaccination schemes.

  10. Parenting practices and adolescent risk behavior: rules on smoking and drinking also predict cannabis use and early sexual debut.

    Science.gov (United States)

    de Looze, Margaretha; van den Eijnden, Regina; Verdurmen, Jacqueline; Vermeulen-Smit, Evelien; Schulten, Ingrid; Vollebergh, Wilma; ter Bogt, Tom

    2012-12-01

    Previous research has provided considerable support for idea that increased parental support and control are strong determinants of lower prevalence levels of adolescent risk behavior. Much less is known on the association between specific parenting practices, such as concrete rules with respect to smoking and drinking and adolescent risk behavior. The present paper examined whether such concrete parental rules (1) have an effect on the targeted behaviors and (2) predict other, frequently co-occurring, risk behaviors (i.e., cannabis use and early sexual intercourse). These hypotheses were tested in a nationally representative sample of 12- to 16-year-old adolescents in the Netherlands. We found that both types of rules were associated with a lower prevalence of the targeted behaviors (i.e., smoking and drinking). In addition, independent of adolescent smoking and drinking behaviors, parental rules on smoking predicted a lower prevalence of cannabis use and early sexual intercourse, and parental rules on alcohol use also predicted a lower prevalence of early sexual intercourse. This study showed that concrete parental rule setting is more strongly related to lower levels of risk behaviors in adolescents compared to the more general parenting practices (i.e., support and control). Additionally, the effects of such rules do not only apply to the targeted behavior but extend to related behaviors as well. These findings are relevant to the public health domain and suggest that a single intervention program that addresses a limited number of concrete parenting practices, in combination with traditional support and control practices, may be effective in reducing risk behaviors in adolescence.

  11. Comparison of single-agent chemotherapy and targeted therapy to first-line treatment in patients aged 80 years and older with advanced non-small-cell lung cancer

    Directory of Open Access Journals (Sweden)

    Zhang QQ

    2015-04-01

    Full Text Available Qianqian Zhang,1 Zhehai Wang,2 Jun Guo,2 Liyan Liu,2 Xiao Han,2 Minmin Li,1 Shu Fang,1 Xiang Bi,1 Ning Tang,1 Yang Liu1 1School of Medicine and Life Sciences, Shandong Academy of Medical Sciences, University of Jinan, 2Department of Oncology, Shandong Cancer Hospital, Jinan, People’s Republic of China Purpose: The aim of this study was to compare single-agent chemotherapy with targeted therapy in initial treatment and to explore a better choice of treatment for patients aged 80 years and older with advanced non-small-cell lung cancer (NSCLC.Patients and methods: A retrospective chart review was conducted for 136 patients aged 80 years and older who were cytopathologically diagnosed and staged as advanced (stage IIIB or IV NSCLC. The patient population was divided into two treatment groups: 78 patients were allocated to the chemotherapy group (group A, pemetrexed or gemcitabine or docetaxel as a single agent, and 60 patients were allocated to another group and received epidermal growth factor-receptor tyrosine-kinase inhibitors (group B, erlotinib or gefitinib as a single agent. The primary end points were overall survival (OS and progression-free survival (PFS, and the secondary end points were response rate, disease-control rate, safety, and quality of life.Results: In group A and group B, respectively, the median PFS was 2 versus 4 months (P=0.013, and the median OS was 8 versus 16 months (P=0.025. The 1- and 2-year survival rates of the two groups were 23.7% (group A, 18 of 76 versus 76.7% (group B, 46 of 60 and 13.2% (group A, ten of 76 versus 10% (group B, six of 60, respectively. The response rate and disease-control rate were 28.9% versus 36.7% (P=0.39 and 57.9% versus 76.7% (P=0.022 in group A and group B, respectively.Conclusion: Elders aged 80 years and over with advanced NSCLC in group B had longer PFS and OS compared with group A. It was well tolerated in group B because of the mild adverse effects. Targeted therapy can be

  12. Parents know best, but are they accurate? Parental normative misperceptions and their relationship to students' alcohol-related outcomes.

    Science.gov (United States)

    LaBrie, Joseph W; Hummer, Justin F; Lac, Andrew; Ehret, Phillip J; Kenney, Shannon R

    2011-07-01

    Parents often look to other parents for guidance, but how accurate are their perceptions? Expanding on existing normative literature to include parents of college students, this study first sought to determine whether parents accurately estimated the attitudes of other parents concerning their college student's alcohol-related behaviors. The effect of these (mis)perceived injunctive norms on the alcohol-related attitudes and behaviors of the parents' own children was then examined. Participants were 270 college student-parent dyadic pairs who completed independent online surveys. The student sample was 59% female; the parent sample was 78% female. A structural equation model demonstrated that parents significantly overestimated other parents' approval of alcohol use by their respective child and, further, that these misperceptions strongly influenced parental attitudes toward their own child's drinking. Parental attitudes were subsequently found to be significantly associated with their child's attitudes toward drinking but were only marginally associated with the child's actual drinking, thereby underscoring the mediational effect of the child's attitudes. This is the first study to document the influence of parental normative misperceptions regarding alcohol use by their college-age children, reinforcing the importance of parental attitudes on children's alcohol-related attitudes and behaviors in college. These findings support the need to complement student-based interventions with parent-based interventions aimed at increasing parental awareness and involvement. Further, the current findings indicate that normative interventions targeting parents offer a promising avenue by which to indirectly and positively influence college students' alcohol use.

  13. Parental responsibility beliefs: associations with parental anxiety and behaviours in the context of childhood anxiety disorders.

    Science.gov (United States)

    Apetroaia, Adela; Hill, Claire; Creswell, Cathy

    2015-12-01

    High levels of parental anxiety are associated with poor treatment outcomes for children with anxiety disorders. Associated parental cognitions and behaviours have been implicated as impediments to successful treatment. We examined the association between parental responsibility beliefs, maternal anxiety and parenting behaviours in the context of childhood anxiety disorders. Anxious and non-anxious mothers of 7-12 year old children with a current anxiety disorder reported their parental responsibility beliefs using a questionnaire measure. Parental behaviours towards their child during a stressor task were measured. Parents with a current anxiety disorder reported a greater sense of responsibility for their child's actions and wellbeing than parents who scored within the normal range for anxiety. Furthermore, higher parental responsibility was associated with more intrusive and less warm behaviours in parent-child interactions and there was an indirect effect between maternal anxiety and maternal intrusive behaviours via parental responsibility beliefs. The sample was limited to a treatment-seeking, relatively high socio-economic population and only mothers were included so replication with more diverse groups is needed. The use of a range of stressor tasks may have allowed for a more comprehensive assessment of parental behaviours. The findings suggest that parental anxiety disorder is associated with an elevated sense of parental responsibility and may promote parental behaviours likely to inhibit optimum child treatment outcomes. Parental responsibility beliefs may therefore be important to target in child anxiety treatments in the context of parental anxiety disorders. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

  14. Target Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — [Part of the ATLAS user facility.] The Physics Division operates a target development laboratory that produces targets and foils of various thickness and substrates,...

  15. Single-molecule Förster resonance energy transfer (FRET) analysis discloses the dynamics of the DNA-topoisomerase II (Top2) interaction in the presence of TOP2-targeting agents.

    Science.gov (United States)

    Huang, Wan-Chen; Lee, Chun-Ying; Hsieh, Tao-Shih

    2017-07-28

    Topoisomerases play crucial roles in DNA replication, transcription, and recombination. For instance, topoisomerase II (Top2) is critically important for resolving DNA tangles during cell division, and as such, it is a broad anticancer drug target. Top2 regulates DNA topology by transiently breaking one double-stranded DNA molecule (cleavage), allowing a second double strand to pass through the opened DNA gate (opening), and then closing the gate by rejoining the broken ends. Drugs that modulate Top2 catalysis may therefore affect enzymatic activity at several different steps. Previous studies have focused on examining DNA cleavage and ligation; however, the dynamic opening and closing of the DNA gate has been less explored. Here, we used the single-molecule Förster resonance energy transfer (smFRET) method to observe the open and closed state of the DNA gate and to measure dwell times in each state. Our results show that Top2 binds and bends DNA to increase the energy transfer efficiency ( E FRET ), and ATP treatment further induces the fluctuation of E FRET , representing the gate opening and closing. Additionally, our results demonstrate that both types of Top2-targeting anticancer drugs, the catalytic inhibitor dexrazoxane (ICRF187) and mechanistic poison teniposide (VM26), can interfere with DNA gate dynamics and shorten the dwell time in the closed state. Moreover, Top2 bound to the nonhydrolyzable ATP analog 5'-adenylyl-β,γ-imidodiphosphate exhibits altered DNA gate dynamics, but the DNA gate appears to open and close even after N-gate closure. In summary, we have utilized single-molecule detection to unravel Top2 DNA gate dynamics and reveal previously unknown effects of Top2 drugs on these dynamics. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  16. Double Jeopardy: Parental Conflict and Stepfamily Outcomes for Children.

    Science.gov (United States)

    Hanson, Thomas L.; And Others

    1996-01-01

    Uses data from the 1987-88 National Survey of Families and Households to examine whether conflict between parents can explain why children in stepfather households do worse than children in original, two-parent households and no better than children in single-mother households. Suggests parental conflict does not account for lower levels of…

  17. Parent Imprisonment and Child Socialization Research Project. Final Report.

    Science.gov (United States)

    Howard Univ., Washington, DC. Dept. of Psychology.

    Conducted in response to the 7% increase in black female single-parent families during 1970 to 1975, this project systematically investigates the impact of parent-absence on the socialization of black children. Divided into four separate studies, the project concentrates on (1) community-social variables related to black parent-absent families;…

  18. Attitudes towards parental presence at induction of anaesthesia

    African Journals Online (AJOL)

    induction of anaesthesia,~Many paediatric anaesthetists today would agree that parental presence at induction is ... personal attitudes to parental presence, perceived benefits for the child and parent, and negative ... the X2 goodness of fit test for a single sample to compare the number of respondents who disagreed with ...

  19. Parental Influences on Adolescent Adjustment: Parenting Styles Versus Parenting Practices

    Science.gov (United States)

    Lee, Sang Min; Daniels, M. Harry; Kissinger, Daniel B.

    2006-01-01

    The study identified distinct patterns of parental practices that differentially influence adolescent behavior using the National Educational Longitudinal Survey (NELS:88) database. Following Brenner and Fox's research model (1999), the cluster analysis was used to classify the four types of parental practices. The clusters of parenting practices…

  20. Measurement of Single Spin Asymmetry in 3He↑(e, e'K±)X from a Transversely Polarized 3He Target

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, Yuxiang [Univ. of Science and Technology, Hefei (China)

    2015-05-01

    Spin-dependent observables are a powerful tool to probe the internal structure of the nucleon and to study the dynamics of the strong interaction. Experimental study of the nucleon spin structure has provided us with many exciting and often surprising results. The so-called "spin crisis" in the 1980s revealed the limitation of naive quark-parton models and led to a worldwide effort to study the nucleon spin structure. However, this effort has been focused mainly on the nucleon's longitudinal spin structure. Recently, when the pioneer work revealed the significant role that transverse spin plays in understanding the full structure of the nucleon and in understanding the dynamics of the strong interaction, the study of the transverse spin structure became the new focus of the worldwide effort. Jefferson Lab (JLab) is located at Newport News, VA, US. It is equipped with the continuous electron beam accelerator facility (CEBAF) and four experimental halls: A, B, C and D. The accelerator can provide a continuous electron beam (2 ns beam bunch) with high polarization (up to ~ 90%) and high current (up to ~ 200μA) for fixed target experiments in all experimental halls. Hall A consists of two standard high-resolution spectrometers (HRS): left HRS (LHRS) and right HRS (RHRS). Another spectrometer, the BigBite spectrometer, can be installed on request by certain experiments. The experiment E06-010 ("Transversity Experiment") at JLab Hall A is the first measurement of the transverse spin structure of the neutron using a transversely polarized 3He target and a 5.89 GeV incident electron beam. The experiment measured target single spin asymmetries (SSA) and beam-target double-pin asymmetries (DSA) in semi-inclusive deep-inelastic scattering (SIDIS) and in deep-inelastic scattering (DIS) processes. It also collected inclusive hadron (pion, kaon and proton) production data parasitically. The scattered electrons were detected in the BigBite spectrometer with

  1. Involving Divorced Parents.

    Science.gov (United States)

    Tarriff, Harold M.; Levine, Valerie

    1993-01-01

    In divorced families, the noncustodial parent is usually as important to the child as the residential parent. Schools should avoid actions that cause parental conflict, place one parent in a sole decision-making role, or deny a parent's access to information or involvement. School responsibilities governing routine correspondence, cyclical and…

  2. The viability of the Parenting Representations Interview for assessing and measuring change in parents of adolescents.

    Science.gov (United States)

    Scharf, Miri; Mayseless, Ofra; Kivenson-Baron, Inbal

    2015-01-01

    Parents' representations include parents' views of their adolescent, of their own parenting and of the parent-adolescent relationship. Two longitudinal studies of parents and their adolescent sons and daughters support the validity of scales coding mothers in the Parenting Representations Interview-Adolescence (PRI-A). The studies, conducted in Israel during the transition from home to military service, demonstrated that three dimensions derived from the PRI-A: positive representations of adolescents, negative emotionality and inadequate boundaries were associated with adolescents' AAI variables, relatedness-autonomy behaviors with mothers, and with other indicators, such as adolescents' wellbeing, romantic intimacy and individuation. Examining parenting representations could help practitioners pinpoint targets for intervention and evaluate the changes that families go through during psychotherapy, as well as the therapeutic process and its outcomes. The findings support the viability of the PRI for use in attachment based interventions for adolescents.

  3. Copenhagen infant mental health project: study protocol for a randomized controlled trial comparing circle of security –parenting and care as usual as interventions targeting infant mental health risks

    Directory of Open Access Journals (Sweden)

    Mette Skovgaard Væver

    2016-11-01

    Full Text Available Abstract Background Infant mental health is a significant public health issue as early adversity and exposure to early childhood stress are significant risk factors that may have detrimental long-term developmental consequences for the affected children. Negative outcomes are seen on a range of areas such as physical and mental health, educational and labor market success, social network and establishing of family. Secure attachment is associated with optimal outcomes in all developmental domains in childhood, and both insecure and disorganized attachment are associated with a range of later problems and psychopathologies. In disadvantaged populations insecure and disorganized attachment are common, which points to the need of identifying early risk and effective methods of addressing such problems. This protocol describes an experimental evaluation of an indicated group-based parental educational program, Circle of Security–Parenting (COS-P, currently being conducted in Denmark. Methods/design In a parallel randomized controlled trial of two intervention groups this study tests the efficacy of COS-P compared to Care as Usual (CAU in enhancing maternal sensitivity and child attachment in a community sample in the City of Copenhagen, Denmark. During the project a general population of an estimated 17.600 families with an infant aged 2–12 months are screened for two known infant mental health risks, maternal postnatal depression and infant social withdrawal. Eligible families (N = 314, who agree to participate, will be randomly allocated with a ratio of 2:1 into the COS-P intervention arm and into CAU. Data will be obtained at inclusion (baseline and at follow-up when the child is 12–16 months. The primary outcome is maternal sensitivity. Secondary outcomes include quality of infant attachment, language, cognitive and socioemotional development, family functioning, parental stress, parental mentalizing and maternal mental wellbeing

  4. Settling for less out of fear of being single.

    Science.gov (United States)

    Spielmann, Stephanie S; MacDonald, Geoff; Maxwell, Jessica A; Joel, Samantha; Peragine, Diana; Muise, Amy; Impett, Emily A

    2013-12-01

    The present research demonstrates that fear of being single predicts settling for less in romantic relationships, even accounting for constructs typically examined in relationship research such as anxious attachment. Study 1 explored the content of people's thoughts about being single. Studies 2A and 2B involved the development and validation of the Fear of Being Single Scale. Study 2C provided preliminary support for the hypothesis that fear of being single predicts settling for less in ongoing relationships, as evidenced by greater dependence in unsatisfying relationships. Study 3 replicated this effect in a longitudinal study demonstrating that fear of being single predicts lower likelihood of initiating the dissolution of a less satisfying relationship. Studies 4A and 4B explored the predictive ability of fear of being single for self-reported dating standards. Across both samples, fear of being single was unrelated to self-reported standards for a mate, with the exception of consistently higher standards for parenting. Studies 5 and 6 explored romantic interest in targets that were manipulated to vary in responsiveness and physical attractiveness. These studies found that fear of being single consistently predicted romantic interest in less responsive and less attractive dating targets. Study 7 explored fear of being single during a speed-dating event. We found that fear of being single predicted being less selective in expressing romantic interest but did not predict other daters' romantic interest. Taken together, the present research suggests that fear of being single is a meaningful predictor of settling for less in relationships. PsycINFO Database Record (c) 2013 APA, all rights reserved.

  5. Parenting while Being Homeless

    Science.gov (United States)

    Swick, Kevin J.; Williams, Reginald; Fields, Evelyn

    2014-01-01

    This article explores the dynamics of parenting while being in a homeless context. The mosaic of stressors involved in this homeless parenting process are explicated and discussed. In addition, resources and strategies that may support parenting are presented and discussed.

  6. Are parents eating their greens?

    DEFF Research Database (Denmark)

    Aschemann-Witzel, Jessica; Bech-Larsen, Tino; Grønhøj, Alice

    2014-01-01

    Purpose - We study the extent of change in parents’ fruit and vegetable consumption during a period when their children participate in a school-based healthy eating intervention. Design/methodology/approach - 256 12-year old Danish schoolchildren took part in a text-message feedback intervention...... promoting fruit and vegetable consumption. One parent of each child filled out self-administered questionnaires at three points during the 40-week long study period. In the questionnaire, stated consumption, perceived influence factors on their consumption and self-efficacy and self-regulation were measured....... Findings - Only half of the parents stated that they met the ‘five a day’ target. These parents reported good availability of fruit and vegetables in their household, high consumption among their friends and frequent exercise and they were characterised by high self-efficacy levels. Stated consumption...

  7. Quantifying Parental Influence on Youth Athlete Specialization: A Survey of Athletes' Parents.

    Science.gov (United States)

    Padaki, Ajay S; Ahmad, Christopher S; Hodgins, Justin L; Kovacevic, David; Lynch, Thomas Sean; Popkin, Charles A

    2017-09-01

    Youth athlete specialization has been linked to decreased enjoyment, burnout, and increased injury risk, although the impact of specialization on athletic success is unknown. The extent to which parents exert extrinsic influence on this phenomenon remains unclear. The goal of this study was to assess parental influences placed on young athletes to specialize. It was hypothesized that parents generate both direct and indirect pressures on specialized athletes. Cross-sectional study; Level of evidence, 3. A survey tool was designed by an interdisciplinary medical team to evaluate parental influence on youth specialization. Surveys were administered to parents of the senior author's orthopaedic pediatric patients. Of the 211 parents approached, 201 (95.3%) completed the assessment tool. One-third of parents stated that their children played a single sport only, 53.2% had children who played multiple sports but had a favorite sport, and 13.4% had children who balanced their multiple sports equally. Overall, 115 (57.2%) parents hoped for their children to play collegiately or professionally, and 100 (49.7%) parents encouraged their children to specialize in a single sport. Parents of highly specialized and moderately specialized athletes were more likely to report directly influencing their children's specialization ( P = .038) and to expect their children to play collegiately or professionally ( P = .014). Finally, parents who hired personal trainers for their children were more likely to believe that their children held collegiate or professional aspirations ( P = .009). Parents influence youth athlete specialization both directly and by investment in elite coaching and personal instruction. Parents of more specialized athletes exert more influence than parents of unspecialized athletes.

  8. Parental overprotection revisited.

    Science.gov (United States)

    Thomasgard, M; Metz, W P

    1993-01-01

    Dimensions of parental overprotection are clarified in a critical review of the research and clinical literature. An indulgent style of parenting is distinguished from an overprotective parent-child relationship. Differential antecedents and outcomes are proposed for each of these forms of parent-child interaction. Measures of protection are reviewed. A new conceptual model of parental overprotection is presented which takes into account child, parent, family, socio-cultural, environmental and resiliency factors. Directions for future research are suggested.

  9. Transgender People (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  10. Cerebral Palsy (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  11. Headaches (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  12. Balance Disorders (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  13. Genital Herpes (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  14. Anemia (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  15. Understanding Puberty (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  16. Diabetes Movie (For Parents)

    Medline Plus

    Full Text Available [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  17. Tips for Divorcing Parents

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  18. Understanding Dyslexia (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  19. When Parents Argue

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  20. Diabetes Movie (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  1. Chlamydia (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  2. Tourette Syndrome (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  3. Sinusitis (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  4. Diarrhea (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  5. Adenovirus (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  6. Birth Defects (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  7. Chemotherapy (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  8. Blood Culture (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  9. Bronchiolitis (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  10. Amblyopia (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  11. Laryngoscopy (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  12. Ultrasound: Head (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  13. Scarlet Fever (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  14. Yersiniosis (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  15. Urine Tests (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  16. Infant Botulism (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  17. Ultrasound: Pelvis (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  18. Eczema (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  19. Amebiasis (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  20. Strep Throat (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  1. Down Syndrome (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  2. Syphilis (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  3. Broken Bones (For Parents)

    Science.gov (United States)

    [Skip to Content] for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family ...

  4. Chinese Parenting Reconsideration: Parenting Practices in Taiwan.

    Science.gov (United States)

    Chen, Fu-mei; Luster, Tom

    This study examined authoritative and authoritarian parenting and specific parenting practices among Chinese mothers with preschoolers. The final sample consisted of 463 mothers with their 3 to 7 year-olds from 11 preschools, in Taiwan. Mothers completed a Chinese translation of the Parenting Behavior Questionnaire that assessed their parenting…

  5. Authoritative parenting and cigarette smoking among multiethnic preadolescents: the mediating role of anti-tobacco parenting strategies.

    Science.gov (United States)

    Stanton, Cassandra A; Highland, Krista B; Tercyak, Kenneth P; Luta, Gheorghe; Niaura, Raymond S

    2014-01-01

    Parenting has been shown to affect smoking among children in U.S. majority groups, but less is known about this association among multiethnic urban populations. Our study examines the role of parenting on smoking among a highly diverse sample. Health surveys were collected from eighth graders (N = 459) in 2 low-income urban schools. Structural equation models examined the direct and indirect effects of authoritative parenting on lifetime smoking. A moderated mediation analysis examined whether indirect effects of authoritative parenting vary among racial/ethnic groups. Authoritative controlling parenting, characterized by limit setting, was positively associated with anti-tobacco parenting. Anti-tobacco parenting was inversely associated with smoking, mediating the relationship between controlling parenting and smoking. There was no evidence that mediation was moderated by race/ethnicity. Parent training, which focuses on setting rules and expectations, can be an important and universal element of smoking prevention programs targeted to youth in diverse communities.

  6. Influence of parental alcohol-related attitudes, behavior and parenting styles on alcohol use in late and very late adolescence.

    Science.gov (United States)

    Stafström, Martin

    2014-01-01

    Parents influence adolescent drinking behavior, but to what extent does this association diminish with age, however? The cross-sectional data was drawn from the Scania drug use survey 2007, consisting of 4,828 secondary education students in the 9th and 11th grade. The age- and gender-adjusted findings indicate that having parents who are consenting to alcohol use (OR 1.4), having been provided with alcohol by one's parents (OR 1.8), having parents with an authoritarian (OR 1.5) or neglectful (OR 2.1) parenting style, and having parents who both have a university degree (OR 1.3) were factors significantly associated with monthly heavy episodic drinking. These findings lead to the conclusion that parenting styles as well as parental attitudes and behaviors are important throughout the high school years. Thus, prevention targeting parents should emphasize both these domains. © 2014 S. Karger AG, Basel.

  7. Targeted Amplicon Sequencing for Single-Nucleotide-Polymorphism Genotyping of Attaching and Effacing Escherichia coli O26:H11 Cattle Strains via a High-Throughput Library Preparation Technique.

    Science.gov (United States)

    Ison, Sarah A; Delannoy, Sabine; Bugarel, Marie; Nagaraja, Tiruvoor G; Renter, David G; den Bakker, Henk C; Nightingale, Kendra K; Fach, Patrick; Loneragan, Guy H

    2016-01-15

    Enterohemorrhagic Escherichia coli (EHEC) O26:H11, a serotype within Shiga toxin-producing E. coli (STEC) that causes severe human disease, has been considered to have evolved from attaching and effacing E. coli (AEEC) O26:H11 through the acquisition of a Shiga toxin-encoding gene. Targeted amplicon sequencing using next-generation sequencing technology of 48 phylogenetically informative single-nucleotide polymorphisms (SNPs) and three SNPs differentiating Shiga toxin-positive (stx-positive) strains from Shiga toxin-negative (stx-negative) strains were used to infer the phylogenetic relationships of 178 E. coli O26:H11 strains (6 stx-positive strains and 172 stx-negative AEEC strains) from cattle feces to 7 publically available genomes of human clinical strains. The AEEC cattle strains displayed synonymous SNP genotypes with stx2-positive sequence type 29 (ST29) human O26:H11 strains, while stx1 ST21 human and cattle strains clustered separately, demonstrating the close phylogenetic relatedness of these Shiga toxin-negative AEEC cattle strains and human clinical strains. With the exception of seven stx-negative strains, five of which contained espK, three stx-related SNPs differentiated the STEC strains from non-STEC strains, supporting the hypothesis that these AEEC cattle strains could serve as a potential reservoir for new or existing pathogenic human strains. Our results support the idea that targeted amplicon sequencing for SNP genotyping expedites strain identification and genetic characterization of E. coli O26:H11, which is important for food safety and public health. Copyright © 2016 Ison et al.

  8. Measurement of single-target spin asymmetries in the electroproduction of negative pions in the semi-inclusive deep inelastic reaction n(e,e'π-)X on a transversely polarized 3He target

    Energy Technology Data Exchange (ETDEWEB)

    Dutta, Chiranjib [Univ. of Kentucky, Lexington, KY (United States)

    2010-06-14

    The experiment E06010 measured the target single spin asymmetry (SSA) in the semiinclusive deep inelastic (SIDIS) n(e,e'π-)X reaction with a transversely polarized 3He target as an e ective neutron target. This is the very rst independent measurement of the neutron SSA, following the measurements at HERMES and COMPASS on the proton and the deuteron. The experiment acquired data in Hall A at Je erson Laboratory with a continuous electron beam of energy 5.9 GeV, probing the valence quark region, with x = 0.13 → 0.41, at Q2 = 1.31 → 3.1 GeV2. The two contributing mechanisms to the measured asymmetry, viz, the Collins effect and the Sivers effect can be realized through the variation of the asymmetry as a function of the Collins and Sivers angles. The neutron Collins and Sivers moments, associated with the azimuthal angular modulations, are extracted from the measured asymmetry for the very first time and are presented in this thesis. The kinematics of this experiment is comparable to the HERMES proton measurement. However, the COMPASS measurements on deuteron and proton are in the low-x region. The results of this experiment are crucial as the first step toward the extraction of quark transversity and Sivers distribution functions in SIDIS. With the existing results on proton and deuteron, these new results on neutron will provide powerful constraints on the transversity and Sivers distributions of both the u and d-quarks in the valence region.

  9. The Identification of Alienated Parents and Children: Implications for School Psychologists.

    Science.gov (United States)

    Calabrese, Raymond L.; And Others

    1987-01-01

    Examined predictors of parental alienation in parents of 49 urban midwestern fourth-graders using the Dean Alienation Scale. High levels of alienation were associated with single, unemployed female parents, whose child was a female with few perceived friends. In predicting parental level of alienation, race, achievement, and attendance were not…

  10. Parental Relationships and Homosexuality

    Science.gov (United States)

    Evans, Ray B.

    1971-01-01

    Confirmed is Bieber's finding (1962) that childhood parental relationships of homosexual men are less desirable than those of heterosexual men. However, while parental impact on children may be greater than the other way around, child impact on the parent probably determines parental attitudes toward that child. (CJ)

  11. Parent Hearing Aid Experiences

    Science.gov (United States)

    Munoz, Karen; Roberts, Mallory; Mullings, Day; Harward, Richard

    2012-01-01

    This study addresses parent experiences in obtaining and managing hearing aids for their young child. The purpose was to identify challenges parents encounter to determine what state agencies can do to improve parent access to amplification. Data were collected July through September of 2010; 40 parents of children ages birth to 3 years old…

  12. Parental and sibling effects in adolescent outcomes.

    Science.gov (United States)

    Moser, Richard P; Jacob, Theodore

    2002-10-01

    This study examined the differential effects of parenting and sibling influences (and the interaction between the two) in predicting adolescents' problem behaviors. The reliability and validity of the Sibling Relationship Questionnaire was also assessed. Subjects were 99 intact families from the San Francisco Bay area, all of whom contained both parents and at least one target child between 10 and 18 years of age still living in the home. Analysis indicated that sibling behavior was a significant predictor of deviant behavior, as measured by the Child Behavior Checklist, even when parenting effects were statistically controlled. Parenting effects had a separate effect on adolescents' positive behaviors. The analysis of the Sibling Relationship Questionnaire indicated adequate test-retest and internal consistency reliability, and construct validity. Findings are discussed in terms of the larger literature on socialization on the different ways that siblings and parents influence adolescent outcomes.

  13. Parental Competences in Psychosocial Risk Context

    Directory of Open Access Journals (Sweden)

    M.ª José Rodrigo López

    2010-01-01

    Full Text Available The analysis and evaluation of parental competences is a central task for the Social Services and specifically for those services targeted at families living in psychosocial risk circumstances. The evaluation of parental competence can be used, among others motives, to take support measures to the family or even to determine if it is necessary to displace the children from his/her home. Despite of its importance, it is an area of sudy underexploited that also plays a secondary role in the field of child protection. For this reason, it is important to clarify what is meant by parental competence, which are the parental competences considered as basic for the healthy and positive parenting of the children, as well as to have clear guidelines to profesionals for carrying out the assessment of these competences.The current article tries to give answers to the questions mentioned.

  14. Children's Divorce and Parent-Child Contact: A Within-Family Analysis of Older European Parents.

    Science.gov (United States)

    Kalmijn, Matthijs

    2016-03-01

    Studies have shown that a parental divorce has a negative effect on parent-child relations. This study examines how adult children's divorce affects the amount of contact children have with older parents, making a distinction between the effects of being single on the one hand and the effects of divorce on the other hand. Using data on older adults in 11 European countries, I estimate within-family regression models to compare multiple adult children per parent (19,454 children aged 30-49; 10,476 parents aged 50-96). I analyze contact frequency while taking into account coresidence and distance. When comparing single divorced children and married children, no difference in contact is observed, but divorced children are more likely to live with their parents. When comparing among children who are single, divorced children have less frequent contact with parents than never-married children. This negative divorce effect exists for sons and daughters and is found in 9 of the 11 countries. The divorce of a child has a double meaning: it leads to being single, which is associated with stronger intergenerational ties, but it is also a non-normative and stressful life event, which is associated with weaker intergenerational ties. © The Author 2014. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  15. Cohabitation: parents following in their children's footsteps?

    Science.gov (United States)

    McClain, Lauren Rinelli

    2011-01-01

    As cohabitation has risen dramatically in the past few decades among adults of all ages, it is possible that middle-and older-aged parents are “learning” cohabitation from their young adult children. The present study uses this theory as a guiding framework to determine if parents are more likely to cohabit themselves following the start of a young adult child’s cohabitation. Using three waves of the National Survey of Families and Households (N = 275), results show that union formation patterns are influenced by young adult children among parents who are single at their child’s 18th birthday. Parents are less likely to marry than remain single and are much more likely to cohabit than marry if they have a young adult child who cohabits. These results show support for the hypotheses.

  16. Maternal depressive symptomatology and parenting behavior: exploration of possible mediators.

    Science.gov (United States)

    Gerdes, Alyson C; Hoza, Betsy; Arnold, L Eugene; Pelham, William E; Swanson, James M; Wigal, Timothy; Jensen, Peter S

    2007-10-01

    Possible mediators of the relation between maternal depressive symptomatology and parenting behavior were examined for 96 children with ADHD and their mothers drawn from the Multimodal Treatment Study of Children with ADHD (MTA) as part of an add-on investigation conducted by two of the six MTA sites. General cognitions (i.e., maternal locus of control and self-esteem) and parenting-specific factors (i.e., maternal parenting efficacy and parenting stress) were examined as possible mediators. Findings provide initial support that maternal parenting stress, as well as maternal locus of control and self-esteem mediate the relation between maternal depressive symptomatology and parenting behavior. This provides support for the argument that some families of children with ADHD may benefit from an expanded version of parent management training that includes sessions directly targeting affective and cognitive factors in parents, similar to treatment programs used to treat childhood conduct problems.

  17. A Parent-to-Parent Program in Taiwan

    Science.gov (United States)

    Liu, Kae

    2018-01-01

    Parent-to-parent programs provide emotional and informational support to parents of children with special needs by matching trained and experienced parents with parents needing support. This study examined the implementation and effects of a Parent-to-Parent Program in Taiwan that supported 3 families of youngsters with special needs. Based on the…

  18. Parents' talk : multiple schemas and parenting practice

    OpenAIRE

    Sarda, Zoltan G.

    2012-01-01

    The impetus for this study is derived from the researcher's experience as a teacher and parent educator. In such contexts, parents frequently lament about the difficulties they experience in developing and sustaining "best practices" in raising their children, and the intransigent nature of existing habits. Much schematic cognition about issues such as relationships involved in parenting resides beneath conscious awareness, and is activated through automatic or habitual interpretations and be...

  19. Parenting and HIV.

    Science.gov (United States)

    Rochat, Tamsen; Netsi, Elena; Redinger, Stephanie; Stein, Alan

    2017-06-01

    With the widespread use of antiretroviral therapy and successful prevention of mother-to-child transmission the development of HIV-negative children with HIV-positive parents has become an important focus. There is considerable evidence that children's developmental risk is heightened because a parental HIV-diagnosis is associated with a range of potential problems such as depression, stigma and financial difficulties. Up to a third of children in sub-Saharan Africa (SSA) are cared for by an HIV-positive parent or caregiver. We review the mechanisms by which HIV affects parenting including its negative effects on parental responsiveness in the early years of parenting and parental avoidant coping styles and parenting deficits in the later years. We describe low-cost parenting interventions suited for low resourced HIV endemic settings. Copyright © 2017. Published by Elsevier Ltd.

  20. What are other parents saying? Perceived parental communication norms and the relationship between alcohol-specific parental communication and college student drinking

    Science.gov (United States)

    Napper, Lucy E.; Hummer, Justin F.; Lac, Andrew; LaBrie, Joseph W.

    2013-01-01

    This study examined parents’ normative perceptions of other college parents’ alcohol-specific communication, and how parents’ perceived communication norms and alcohol-specific communication relate to student drinking outcomes. A sample of 457 student-parent dyads were recruited from a mid-size university. Students completed web-based assessments of alcohol-related attitudes and behaviors. Parents completed alcohol-specific measures of communication norms and parent-child communication, including communication content (i.e., targeted communication) and frequency of communication. Results indicated that parents overestimated how much other parents talked to their college students about the frequency and quantity of alcohol use, but underestimated how often parents initiated conversations about alcohol. In a path model, perceived communication norms positively predicted both targeted communication and frequency of communication. Perceived communication norms and targeted communication negatively predicted students’ attitude toward alcohol use. In contrast, more frequent communication predicted students holding more approving attitudes toward alcohol. The relationship between parents’ perceived communication norms and students’ drinking behaviors was mediated by the parental communication variables and student attitudes. Tests of indirect effects were undertaken to examine meditational processes. The findings underscore relations involving parental perceived communication norms and parents’ own alcohol communication and their children’s drinking outcomes. The complex relationships of different types of parental communication and student outcomes warrant further research. PMID:24128293

  1. Parenting for Yourself and Your Child. A Parenting Curriculum for High Risk Families. Neglectful and Abusive Parents: Curriculum Development and Pilot Program. Final Report.

    Science.gov (United States)

    Bourque, Janet

    Developed for use by parent educators and others working with high risk, abusive, or neglectful families, this curriculum guide is intended to enable and facilitate the growth of this target population in key parenting learning and skill areas. Section 1 provides an overview of the manual, offers suggestions for home visits following each class…

  2. Molecular Targets for Targeted Radionuclide Therapy

    International Nuclear Information System (INIS)

    Mather, S.J.

    2009-01-01

    radiolabelled regulatory peptides and their metabolically stabilised analogues. Antigen epitopes: Antibodies, as unlabelled biological drugs, are becoming of increasing interest. They exert an antibody-dependent cellular cytotoxicity which leads to lysis of tumour cells. Radiolabelled versions of these (and other) antibodies are being developed worldwide. The disadvantage of the long circulating time of antibodies can be solved by engineering fragments such as diabodies, bivalent single chain variable fragments (scFv), minibodies or by pretargeting approaches. Transmembrane transporters: Other interesting targets are transporters for radiolabelled amino acids and nutrients. Cancer cells require an increased supply of many such nutrients and obtain these by increased expression of some types of amino-acid transporter. A more detailed analysis of the relationship between amino-acid uptake and transporter expression in normal and malignant cells would be very valuable in identifying the clinical therapeutic potential of this class of tracer. Tumour blood supply: Tumours require an efficient blood supply to grow and metastatise and active angiogenesis of new blood vessels is a feature of many tumours. Specific receptors expressed during this process represent a novel class of targets for TRT. Extra-cellular matrix: Recently, another relevant class of target antigens has raised interest. Lectins, or carbohydrate binding proteins, recognize specific oligosaccharide structures on glycoproteins and glycolipids. It is well known that protein and lipid glycosylation are consistently altered in cancer cells for the aberrant activity of specific glycosyltransferase and glycosydases. Experimental evidence demonstrated that tumor growth and progression may depend, at least in part, on the presence of altered glycoproteins on the cell surface, which can mediate aberrant receptor-ligand interactions. (author)

  3. The effectiveness of universal parenting programmes: the CANparent trial.

    Science.gov (United States)

    Lindsay, Geoff; Totsika, Vasiliki

    2017-10-23

    There is substantial evidence for the efficacy and effectiveness of targeted parenting programmes but much less evidence regarding universal parenting programmes. The aim of the present study was to evaluate the effectiveness of the CANparent Trial of 12 universal parenting programmes, which were made available to parents of all children aged 0-6 years in three local authorities in England. To the best of our knowledge, this is the first study of universal parenting programmes on this scale. Parents accessed a voucher, value £100, to attend an accredited programme of parenting classes. Parents completed measures of their mental well-being, parenting efficacy, parenting satisfaction, and parenting stress, at pre- and post-course. Comparative data were derived from a sample of non-participant parents in 16 local authorities not providing CANparent programmes. A quasi-experimental design was adopted following estimation of propensity scores to balance the two groups on socio-demographic variables. Following their programme, changes in parenting stress were small and nonsignificant (Cohen's d frequency 0.07; intensity, 0.17). Participating parents showed significantly greater improvements than the comparison group for parenting efficacy (0.89) but not parenting satisfaction (-0.01). Mental well-being improved from 0.29 SD below the national norm to the national norm after the course. Parents were overwhelmingly positive about their course (88-94%) but this was lower for improvement in their relationship with their child (74%) and being a better parent (76%). The CANparent Trial demonstrated that universal parenting programmes can be effective in improving parents' sense of parenting efficacy and mental well-being when delivered to the full range of parents in community settings. However, there was no evidence of a reduction in levels of parenting stress; nor was there a significant improvement in satisfaction with being a parent. This is the first study of its kind

  4. Normative feedback for parents of college students: piloting a parent based intervention to correct misperceptions of students' alcohol use and other parents' approval of drinking.

    Science.gov (United States)

    Labrie, Joseph W; Napper, Lucy E; Hummer, Justin F

    2014-01-01

    Multi-component parent-based interventions (PBIs) provide a promising avenue for targeting alcohol use and related consequences in college students. Parents of college-aged children can have a significant influence on their children's alcohol use decisions. However, parents tend to underestimate their own child's alcohol use and overestimate other similar parents' approval of student drinking. These misperceptions could have important implications for parents' own attitudes and alcohol-related communication with their student. Targeting these misperceptions through normative feedback could help promote greater and more in-depth alcohol-related communication. The present study examines the potential efficacy of web-based alcohol-related normative feedback for parents of college students. A sample of 144 parents of college students received web-based normative feedback about students' alcohol use and approval, as well as other same-college parents' alcohol approval. Parents completed measures of perceived student alcohol use, student alcohol approval, other-parent alcohol approval, and intentions to discuss alcohol use both pre- and post-normative feedback. Post-feedback, parents reported stronger intentions to talk to their student about alcohol, were less confident in their knowledge of their students' alcohol use, and believed that their student drank in greater quantity and more frequently than pre-feedback. Parents also perceived other parents to be less approving of alcohol use after viewing normative feedback. These findings provide preliminary support for the use of web-based normative feedback for parents of college students. Given these promising results, further research developing and testing this approach merits attention. © 2013.

  5. Immunotherapy Targets Common Cancer Mutation

    Science.gov (United States)

    In a study of an immune therapy for colorectal cancer that involved a single patient, researchers identified a method for targeting the cancer-causing protein produced by a mutant form of the KRAS gene.

  6. Elevated atmospheric ozone increases concentration of insecticidal Bacillus thuringiensis (Bt) Cry1Ac protein in Bt Brassica napus and reduces feeding of a Bt target herbivore on the non-transgenic parent

    Energy Technology Data Exchange (ETDEWEB)

    Himanen, Sari J. [University of Kuopio, Department of Environmental Science, P.O. Box 1627, FIN-70211 Kuopio (Finland)], E-mail: sari.himanen@uku.fi; Nerg, Anne-Marja [University of Kuopio, Department of Environmental Science, P.O. Box 1627, FIN-70211 Kuopio (Finland); Nissinen, Anne [University of Kuopio, Department of Environmental Science, P.O. Box 1627, FIN-70211 Kuopio (Finland); MTT Agrifood Research Finland, Plant Protection, FIN-31600 Jokioinen (Finland); Stewart, C. Neal [University of Tennessee, Department of Plant Sciences, Knoxville, TN 37996-4561 (United States); Poppy, Guy M. [University of Southampton, School of Biological Sciences, Southampton SO16 7PX (United Kingdom); Holopainen, Jarmo K. [University of Kuopio, Department of Environmental Science, P.O. Box 1627, FIN-70211 Kuopio (Finland)

    2009-01-15

    Sustained cultivation of Bacillus thuringiensis (Bt) transgenic crops requires stable transgene expression under variable abiotic conditions. We studied the interactions of Bt toxin production and chronic ozone exposure in Bt cry1Ac-transgenic oilseed rape and found that the insect resistance trait is robust under ozone elevations. Bt Cry1Ac concentrations were higher in the leaves of Bt oilseed rape grown under elevated ozone compared to control treatment, measured either per leaf fresh weight or per total soluble protein of leaves. The mean relative growth rate of a Bt target herbivore, Plutella xylostella L. larvae was negative on Bt plants in all ozone treatments. On the non-transgenic plants, larval feeding damage was reduced under elevated ozone. Our results indicate the need for monitoring fluctuations in Bt toxin concentrations to reveal the potential of ozone exposure for altering dosing of Bt proteins to target and non-target herbivores in field environments experiencing increasing ozone pollution. - Elevated atmospheric ozone can induce fluctuations in insecticidal protein concentrations in transgenic plants.

  7. Elevated atmospheric ozone increases concentration of insecticidal Bacillus thuringiensis (Bt) Cry1Ac protein in Bt Brassica napus and reduces feeding of a Bt target herbivore on the non-transgenic parent

    International Nuclear Information System (INIS)

    Himanen, Sari J.; Nerg, Anne-Marja; Nissinen, Anne; Stewart, C. Neal; Poppy, Guy M.; Holopainen, Jarmo K.

    2009-01-01

    Sustained cultivation of Bacillus thuringiensis (Bt) transgenic crops requires stable transgene expression under variable abiotic conditions. We studied the interactions of Bt toxin production and chronic ozone exposure in Bt cry1Ac-transgenic oilseed rape and found that the insect resistance trait is robust under ozone elevations. Bt Cry1Ac concentrations were higher in the leaves of Bt oilseed rape grown under elevated ozone compared to control treatment, measured either per leaf fresh weight or per total soluble protein of leaves. The mean relative growth rate of a Bt target herbivore, Plutella xylostella L. larvae was negative on Bt plants in all ozone treatments. On the non-transgenic plants, larval feeding damage was reduced under elevated ozone. Our results indicate the need for monitoring fluctuations in Bt toxin concentrations to reveal the potential of ozone exposure for altering dosing of Bt proteins to target and non-target herbivores in field environments experiencing increasing ozone pollution. - Elevated atmospheric ozone can induce fluctuations in insecticidal protein concentrations in transgenic plants

  8. Parenting children with down syndrome: An analysis of parenting styles, parenting dimensions, and parental stress.

    Science.gov (United States)

    Phillips, B Allyson; Conners, Frances; Curtner-Smith, Mary Elizabeth

    2017-09-01

    Effective parenting is vital for a child's development. Although much work has been conducted on parenting typically developing children, little work has examined parenting children with Down syndrome. The purpose of the current study was to compare the parenting styles and dimensions in mothers of children with DS and mothers of TD children. Thirty-five mothers of children with DS and 47 mothers of TD children completed questionnaires about parenting, parental stress, child behavior problems, and child executive function. We found that mothers of children with DS use an authoritative parenting style less and a permissive parenting style more than mothers of TD children. Additionally, we found that mothers of children with DS use reasoning/induction and verbal hostility less and ignoring misbehavior more than mothers of TD children. All of these differences, except for those of reasoning/induction, were at least partially accounted for by the higher levels of parental stress in the DS group. Parenting interventions should be focused on reducing parental stress and training mothers to parent under stress in an effort to improve parenting techniques, which would, in theory, improve long-term child outcomes for children with DS. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. The relationship between parental depressive symptoms, family type, and adolescent functioning.

    Directory of Open Access Journals (Sweden)

    Dominik Sebastian Sieh

    Full Text Available It is evident that parental depressive symptoms negatively influence adolescent behavior and various psychosocial outcomes. Certain family types like families with a chronically ill parent and single parent families are more vulnerable to parental depressive symptoms. However, the relationship between these symptoms, family type, and adolescent functioning remains largely unclear. This study examined relations between self-report of parental depressive symptoms and adolescent functioning in 86 two-parent families including a parent with a chronic medical condition, 94 families with healthy single parents, and 69 families with 2 healthy parents (comparison group. Parents completed the Beck Depression Inventory. Adolescents filled in the Youth Self-Report measuring problem behavior, and other instruments measuring psychosocial outcomes (stress, grade point average, school problems, and self-esteem. Multilevel analyses were used to examine the effects of family type, parental depressive symptoms, adolescents' gender and age, and interaction effects on adolescent functioning. The results indicated that adolescents with chronically ill and single parents had a lower grade point average (p<.01 than the comparison group. Adolescents of single parents reported more internalizing problems (p<.01 and externalizing problems (p<.05 than children from the other family types. Parental depressive symptoms were strongly related to child report of stress (p<.001. Adolescents of depressed chronically ill parents were particularly vulnerable to internalizing problems (interaction effect, p<.05. Older children and girls, and especially older girls, displayed more internalizing problems and stress. It can be concluded that growing up with a chronically ill parent in a family with 2 parents may have less impact on adolescent problem behavior than growing up in a single parent family. Health practitioners are encouraged to be attentive to the unique and combined

  10. Maternal Personality, Parenting Cognitions, and Parenting Practices

    Science.gov (United States)

    Bornstein, Marc H.; Hahn, Chun-Shin; Haynes, O. Maurice

    2011-01-01

    A community sample of 262 European American mothers of firstborn 20-month-olds completed a personality inventory and measures of parenting cognitions (knowledge, self-perceptions, and reports about behavior) and was observed in interaction with their children from which measures of parenting practices (language, sensitivity, affection, and play)…

  11. Parent training support for intellectually disabled parents.

    Science.gov (United States)

    Coren, Esther; Hutchfield, Jemeela; Thomae, Manuela; Gustafsson, Carina

    2010-06-16

    Intellectual disability may impact on an individual's capacity to parent a child effectively. Research suggests that the number of intellectually disabled people with children is increasing. Children of parents with intellectual disabilities may be at increased risk of neglectful care which could lead to health, developmental and behavioural problems, or increased risk of intellectual disability.However, there is some indication that some parents with intellectual disabilities are able to provide adequate child care if they are given appropriate training and support to do so. To assess the effectiveness of parent training interventions to support the parenting of parents with intellectual disabilities We searched the following databases: Cochrane Central Register of Controlled Trials (CENTRAL, The Cochrane Library), MEDLINE, EMBASE, CINAHL, PsycINFO, ASSIA, Sociological Abstracts, Dissertation Abstracts International, MetaRegister of Controlled Trials, and ZETOC. Randomised controlled trials comparing parent training interventions for parents with intellectual disabilities with usual care or with a control group. Outcomes of interest were: the attainment of parenting skills specific to the intervention, safe home practices and the understanding of child health. Two review authors independently assessed risk of bias and undertook data extraction. Three trials met the inclusion criteria for this review but no meta-analysis was possible. One study reported improved maternal-child interaction following group parent training compared with the control group. The second study reported some improvements in parents knowledge of life threatening emergencies, ability to recognise dangers and identify precautions and smaller improvements in their ability to implement precautions, use medicines safely and recognise child illness and symptoms. The third study reported improvement in child care and safety skills following the intervention. There is some risk of bias in the

  12. Antiproton Target

    CERN Multimedia

    1980-01-01

    Antiproton target used for the AA (antiproton accumulator). The first type of antiproton production target used from 1980 to 1982 comprised a rod of copper 3mm diameter and 120mm long embedded in a graphite cylinder that was itself pressed into a finned aluminium container. This assembly was air-cooled and it was used in conjunction with the Van der Meer magnetic horn. In 1983 Fermilab provided us with lithium lenses to replace the horn with a view to increasing the antiproton yield by about 30%. These lenses needed a much shorter target made of heavy metal - iridium was chosen for this purpose. The 50 mm iridium rod was housed in an extension to the original finned target container so that it could be brought very close to the entrance to the lithium lens. Picture 1 shows this target assembly and Picture 2 shows it mounted together with the lithium lens. These target containers had a short lifetime due to a combination of beam heating and radiation damage. This led to the design of the water-cooled target in...

  13. Parental Stress Scale: Validation study with a Portuguese population of parents of children from 3 to 10 years old.

    Science.gov (United States)

    Algarvio, Susana; Leal, Isabel; Maroco, João

    2018-01-01

    The aim of this study was to validate the Parental Stress Scale (PSS) for Portuguese parents and to further investigate the scale's criterion-related validity. A two-stage stratified sample of the Portuguese population of parents, with children attending public preschools and primary schools, was obtained, totalizing 3842 parents of children between 3 and 10 years old. Parents completed a Parental Concerns Scale and the Portuguese version of the PSS. Results support the four-factor structure of the Portuguese version of the PSS. Higher levels of parental stress were reported by parents of boys, with lower educational levels; older, divorced or single parents; unemployed mothers; and with a higher number of children. Parental concerns and parental stress' comparative study reported very low correlations between the two constructs. This study supported evidence for the PSS' validity with a stratified sample of Portuguese parents of children between 3 and 10 years old. Moreover, our findings reported the scale's divergent validity with a Parental Concerns Scale. These results point to the importance of assessing both dimensions in family practice.

  14. Associations between child emotional eating and general parenting style, feeding practices, and parent psychopathology.

    Science.gov (United States)

    Braden, Abby; Rhee, Kyung; Peterson, Carol B; Rydell, Sarah A; Zucker, Nancy; Boutelle, Kerri

    2014-09-01

    Emotional eating is the tendency to eat in response to negative emotions. Prior research has identified a relationship between parenting style and child emotional eating, but this has not been examined in clinical samples. Furthermore, the relationship between specific parenting practices (e.g., parent feeding practices) and child emotional eating has not yet been investigated. The current study examined relationships between child emotional eating and both general and specific parenting constructs as well as maternal symptoms of depression and binge eating among a treatment-seeking sample of overweight children. Participants included 106 mother-child dyads who attended a baseline assessment for enrollment in a behavioral intervention for overeating. Ages of children ranged from 8 to 12  years old. Mothers completed self-report measures of their child's emotional eating behavior, their own feeding practices, and symptoms of depression and binge eating. Children completed a self-report measure of their mothers' general parenting style. A stepwise regression analysis was conducted to identify the parent variable that was most strongly related to child emotional eating, controlling for child age and gender. Emotional feeding behavior (i.e., a tendency to offer food to soothe a child's negative emotions) was the parent factor most significantly related to child emotional eating. Findings suggest that emotional feeding practices in parents may be related to emotional eating in children. Treatment with overweight children who engage in emotional eating may be improved by targeting parent feeding practices. Copyright © 2014 Elsevier Ltd. All rights reserved.

  15. Effects of Parenting Style and Parent-Related Weight and Diet on Adolescent Weight Status

    Science.gov (United States)

    Wilson, Dawn K.; St. George, Sara M.; Schneider, Elizabeth; Kitzman-Ulrich, Heather

    2013-01-01

    Objective This study examined the interaction between parental limit setting of sedentary behaviors and health factors (weight status, physical activity [PA], fruit and vegetable [FV] intake) on standardized body mass index (zBMI) in African American adolescents. Methods Data were from 67 parent–adolescent dyads. Parental limit setting, PA and FV intake were assessed via self-report, and objective height and weight measurements were collected. Results Regressions examined the interaction between parental limit setting and BMI, PA, FV intake on adolescent zBMI. The model for parent BMI and FV intake accounted for 31% of the variance in adolescent zBMI. A significant interaction for parent BMI by limit setting showed that as parental BMI increased, higher (vs. lower) limit setting was associated with lower adolescent zBMI. Higher parent FV consumption was associated with lower adolescent zBMI. Conclusion Future interventions should integrate parent limit setting and target parent fruit and vegetable intake for obesity prevention in underserved adolescents. PMID:23248345

  16. Parenting and juvenile delinquency

    NARCIS (Netherlands)

    Hoeve, M.

    2008-01-01

    Juvenile delinquency is a noteworthy problem. This thesis addressed the association between parenting and juvenile delinquency by analyzing the concepts of parenting adopted in family research in relation to criminological concepts and measures of delinquent behavior. Four studies were conducted.

  17. Diabetes Movie (For Parents)

    Medline Plus

    Full Text Available ... for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & Fitness Emotions & Behavior School & Family Life First Aid & Safety Doctors & Hospitals Videos Recipes for Kids Kids site ...

  18. Parenting in sport.

    Science.gov (United States)

    Knight, Camilla J; Berrow, Steffan R; Harwood, Chris G

    2017-08-01

    This paper provides a brief summary and commentary on the growing literature on parenting in sport, with a particular emphasis on literature from the last 2-3 years. Following a brief introduction overviewing the topic area, we firstly focus on the influence of parental involvement on children. Specifically, we examine the range of factors that influence children's perceptions of parental involvement and the consequences of different behaviors. Next we discuss the factors influencing parental involvement, such as the challenges and stressors associated with parenting children in sport and the culture within different sports. Finally, our review focuses upon the strategies developed by parents to facilitate their involvement in their children's sport, as well as the few papers focused upon parent education and support. We conclude by examining the need for further research and examination of support strategies for parents. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

  19. Separation Anxiety (For Parents)

    Science.gov (United States)

    ... Safe Videos for Educators Search English Español Separation Anxiety KidsHealth / For Parents / Separation Anxiety What's in this ... both of you get through it. About Separation Anxiety Babies adapt pretty well to other caregivers. Parents ...

  20. Diabetes Movie (For Parents)

    Medline Plus

    Full Text Available ... for Parents Parents site Sitio para padres General Health Growth & Development Infections Diseases & Conditions Pregnancy & Baby Nutrition & ... Growing Up Staying Healthy Staying Safe Recipes & Cooking Health Problems Illnesses & Injuries Relax & Unwind People, Places & Things ...

  1. The effectiveness of universal parenting programmes: the CANparent trial

    Directory of Open Access Journals (Sweden)

    Geoff Lindsay

    2017-10-01

    Full Text Available Abstract Background There is substantial evidence for the efficacy and effectiveness of targeted parenting programmes but much less evidence regarding universal parenting programmes. The aim of the present study was to evaluate the effectiveness of the CANparent Trial of 12 universal parenting programmes, which were made available to parents of all children aged 0–6 years in three local authorities in England. To the best of our knowledge, this is the first study of universal parenting programmes on this scale. Methods Parents accessed a voucher, value £100, to attend an accredited programme of parenting classes. Parents completed measures of their mental well-being, parenting efficacy, parenting satisfaction, and parenting stress, at pre- and post-course. Comparative data were derived from a sample of non-participant parents in 16 local authorities not providing CANparent programmes. A quasi-experimental design was adopted following estimation of propensity scores to balance the two groups on socio-demographic variables. Results Following their programme, changes in parenting stress were small and nonsignificant (Cohen’s d frequency 0.07; intensity, 0.17. Participating parents showed significantly greater improvements than the comparison group for parenting efficacy (0.89 but not parenting satisfaction (−0.01. Mental well-being improved from 0.29 SD below the national norm to the national norm after the course. Parents were overwhelmingly positive about their course (88–94% but this was lower for improvement in their relationship with their child (74% and being a better parent (76%. Conclusions The CANparent Trial demonstrated that universal parenting programmes can be effective in improving parents’ sense of parenting efficacy and mental well-being when delivered to the full range of parents in community settings. However, there was no evidence of a reduction in levels of parenting stress; nor was there a significant improvement in

  2. Parent training interventions for Attention Deficit Hyperactivity Disorder (ADHD) in children aged 5 to 18 years.

    Science.gov (United States)

    Zwi, Morris; Jones, Hannah; Thorgaard, Camilla; York, Ann; Dennis, Jane A

    2011-12-07

    meta-analysis were limited and most data that we have reported are based on single studies. We found five studies including 284 participants that met the inclusion criteria, all of which compared parent training with de facto treatment as usual (TAU). One study included a nondirective parent support group as a second control arm.  Four studies targeted children's behaviour problems and one assessed changes in parenting skills. Of the four studies targeting children's behaviour, two focused on behaviour at home and two focused on behaviour at school. The two studies focusing on behaviour at home had different findings: one found no difference between parent training and treatment as usual, whilst the other reported statistically significant results for parent training versus control. The two studies of behaviour at school also had different findings: one study found no difference between groups, whilst the other reported positive results for parent training when ADHD was not comorbid with oppositional defiant disorder. In this latter study, outcomes were better for girls and for children on medication.We assessed the risk of bias in most of the studies as unclear at best and often as high. Information on randomisation and allocation concealment did not appear in any study report. Inevitably, blinding of participants or personnel was impossible for this intervention; likewise, blinding of outcome assessors (who were most often the parents who had delivered the intervention) was impossible.We were only able to conduct meta-analysis for two outcomes: child 'externalising' behaviour (a measure of rulebreaking, oppositional behaviour or aggression) and child 'internalising' behaviour (for example, withdrawal and anxiety). Meta-analysis of three studies (n = 190) providing data on externalising behaviour produced results that fell short of statistical significance (SMD -0.32; 95% CI -0.83 to 0.18, I(2) = 60%). A meta-analysis of two studies (n = 142) for internalising

  3. Parenting style impacts cognitive and behavioural outcomes of former preterm infants: A systematic review.

    Science.gov (United States)

    Neel, M L M; Stark, A R; Maitre, N L

    2018-03-25

    We sought to evaluate published evidence in aggregate regarding the impact of parenting style on the cognitive and behavioural outcomes of former preterm infants. We searched 5 databases using germane MeSH terms. Parenting style was defined as any descriptor of parenting using ≥2 dimensions on published parenting axes. We evaluated studies for quality of evidence and strength of recommendations using standardized tools and categorized summative recommendations by parenting axis and child outcome. Twenty-seven articles met our inclusion criteria. Parental responsivity is the only parenting axis strongly associated with both improved child cognition and behaviour. Parental demandingness is associated only with improved child cognition, and parental warmth and rejection are associated only with child behaviour. Parental coercion is not associated with subsequent child outcomes. Parental responsivity may be essential in optimizing neurodevelopment in former preterm infants. More targeted studies are needed to inform this relationship and identify opportunities for intervention. © 2018 John Wiley & Sons Ltd.

  4. Parental evaluation of processes of care in relation to the child, parent and family characteristics.

    Science.gov (United States)

    Groleger Sršen, Katja; Vidmar, Gaj; Sočan, Gregor; Zupan, Anton

    2014-09-01

    The Measure of Processes of Care (MPOC) is a questionnaire for parents used to evaluate the behaviours of healthcare providers. We applied its 20-item version (MPOC-20) to explore the associations between parental evaluation of processes of care and child, parent and family characteristics in Slovenia. A novelty of our approach was the emphasis on the role of a key person. Parents of 235 children who were admitted as inpatients or outpatients of six institutions (hospitals and health centres) because of chronic illness or disability participated in the study. Parents were asked to fill in a general questionnaire on several characteristics of the child, child's health problems, the family and the therapy programmes, and MPOC-20. Univariate associations of the five MPOC-20 scale scores with child, parent and family characteristics were tested first. Multiple linear regression was used for modelling scale scores in relation to child, parent and family characteristics. The analyses singled out availability of a key person as the factor most consistently and unequivocally influencing parental satisfaction. We also found a general positive effect of male sex of the child on the MPOC-20 scores. Neither the present age of children nor age at the onset of health problems was found to be associated with MPOC-20 scores. We found no notable association of the number of health problems with the MPOC-20 scores, but observed clear differences when comparing parental satisfaction with processes of care between different participating institutions.

  5. Parenting after Infertility

    Science.gov (United States)

    Olshansky, Ellen

    2009-01-01

    Becoming a parent after experiencing infertility can pose unique challenges to early parenthood. Parents may struggle with the normal anxiety and fatigue, as well as possible depression, that accompany new parenthood, but with added guilt or shame because of how much they wanted a child and how hard they worked to become parents. These feelings…

  6. Cultural Influences on Parenting

    Science.gov (United States)

    Spicer, Paul

    2010-01-01

    ZERO TO THREE's "Parenting Infants and Toddlers Today" survey presents an opportunity to explore areas where African American, Hispanic, and White parents may differ in their perceptions of infant development. The article highlights some of the differences in these racial and ethnic groups, such as parents' understanding of early social and…

  7. The Parent Teacher Partnership.

    Science.gov (United States)

    Barsch, Ray H.

    The text is primarily intended to encourage a particular attitude among teachers to guide them in their relationships with parents, and secondarily to be useful directly to parents. Through an analysis of the parent teacher conference relationship, the author presents particular concepts and outlines a plan of action. Personal dynamics of both the…

  8. The Parent Loan Trap

    Science.gov (United States)

    Wang, Marian; Supiano, Beckie; Fuller, Andrea

    2012-01-01

    As the cost of college has spiraled ever upward and median family income has fallen, the loan program, called Parent PLUS, has become indispensable for increasing numbers of parents desperate to make their children's college plans work. Last year the government disbursed $10.6-billion in Parent PLUS loans to just under a million families. Even…

  9. Parenting Beliefs, Parental Stress, and Social Support Relationships

    Science.gov (United States)

    Respler-Herman, Melissa; Mowder, Barbara A.; Yasik, Anastasia E.; Shamah, Renee

    2012-01-01

    The present study built on prior research by examining the relationship of parental stress and social support to parenting beliefs and behaviors. A sample of 87 parents provided their views concerning the importance of parenting characteristics as well as their level of parental stress and perceived social support. These parents completed the…

  10. 5 CFR 1651.7 - Parent or parents.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Parent or parents. 1651.7 Section 1651.7 Administrative Personnel FEDERAL RETIREMENT THRIFT INVESTMENT BOARD DEATH BENEFITS § 1651.7 Parent or parents. If the account is to be paid to the participant's parent or parents under § 1651.2(a)(4), the following...

  11. Evaluation of a Blog Based Parent Involvement Approach by Parents

    Science.gov (United States)

    Ozcinar, Zehra; Ekizoglu, Nihat

    2013-01-01

    Despite the well-known benefits of parent involvement in children's education, research clearly shows that it is difficult to effectively involve parents. This study aims to capture parents' views of a Blog Based Parent Involvement Approach (BPIA) designed to secure parent involvement in education by strengthening school-parent communication. Data…

  12. Personality and Parenting Style in Parents of Adolescents

    Science.gov (United States)

    Huver, Rose M. E.; Otten, Roy; de Vries, Hein; Engels, Rutger C. M. E.

    2010-01-01

    Since parental personality traits are assumed to play a role in parenting behaviors, the current study examined the relation between parental personality and parenting style among 688 Dutch parents of adolescents in the SMILE study. The study assessed Big Five personality traits and derived parenting styles (authoritative, authoritarian,…

  13. Lost in translation: a focus group study of parents' and adolescents' interpretations of underage drinking and parental supply.

    Science.gov (United States)

    Jones, Sandra C; Andrews, Kelly; Berry, Nina

    2016-07-13

    Reductions in underage drinking will only come about from changes in the social and cultural environment. Despite decades of messages discouraging parental supply, parents perceive social norms supportive of allowing children to consume alcohol in 'safe' environments. Twelve focus groups conducted in a regional community in NSW, Australia; four with parents of teenagers (n = 27; 70 % female) and eight with adolescents (n = 47; 55 % female). Participants were recruited using local media. Groups explored knowledge and attitudes and around alcohol consumption by, and parental supply of alcohol to, underage teenagers; and discussed materials from previous campaigns targeting adolescents and parents. Parents and adolescents perceived teen drinking to be a common behaviour within the community, but applied moral judgements to these behaviours. Younger adolescents expressed more negative views of teen drinkers and parents who supply alcohol than older adolescents. Adolescents and parents perceived those who 'provide alcohol' (other families) as bad parents, and those who 'teach responsible drinking' (themselves) as good people. Both groups expressed a preference for high-fear, victim-blaming messages that targeted 'those people' whose behaviours are problematic. In developing and testing interventions to address underage drinking, it is essential to ensure the target audience perceive themselves to be the target audience. If we do not have a shared understanding of underage 'drinking' and parental 'provision', such messages will continue to be perceived by parents who are trying to do the 'right' thing as targeting a different behaviour and tacitly supporting their decision to provide their children with alcohol.

  14. Intergenerational Transmission of Harsh Parenting.

    Science.gov (United States)

    Simons, Ronald L.; And Others

    1991-01-01

    Examined harsh parenting across generations by means of parents' and adolescents' reports. Found that grandparents who had engaged in aggressive parenting produced parents who used similar practices. Harsh discipline of male children was a function of socioeconomic characteristics. (BC)

  15. A Comparison of Discipline Techniques of Daycare and Nondaycare Parents.

    Science.gov (United States)

    Golub, Judith S.; And Others

    This study describes discipline practices, attitudes, beliefs, and other characteristics that may differentiate parents of children in day care from those parents who care for their children at home. Adult-child ratio, staff continuity, and staff training were used as quality-of-care indices. A total of 89 families with target children between 18…

  16. Friendship Network in the Classroom: Parents Bias and Peer Effects

    DEFF Research Database (Denmark)

    Landini, Fabio; Montinari, Natalia; Pin, Paolo

    : parents expect peer effects on school achievement to be stronger than what they really are. Thus, parents of low-performing students report their children to be friends of high-performing students. Our numerical simulations indicate that when this bias is combined with a bias on how some children target...

  17. The agreement between parent-reported and directly measured child language and parenting behaviors

    Directory of Open Access Journals (Sweden)

    Shannon K Bennetts

    2016-11-01

    Full Text Available Parenting behaviors are commonly targeted in early interventions to improve children’s language development. Accurate measurement of both parenting behaviors and children’s language outcomes is thus crucial for sensitive assessment of intervention outcomes. To date, only a small number of studies have compared parent-reported and directly measured behaviors, and these have been hampered by small sample sizes and inaccurate statistical techniques, such as correlations. The Bland-Altman Method and Reduced Major Axis regression represent more reliable alternatives because they allow us to quantify fixed and proportional bias between measures. In this study, we draw on data from two Australian early childhood cohorts (N= 201 parents and slow-to-talk toddlers aged 24 months; and N=218 parents and children aged 6-36 months experiencing social adversity to (1 examine agreement and quantify bias between parent-reported and direct measures, and (2 to determine socio-demographic predictors of the differences between parent-reported and direct measures. Measures of child language and parenting behaviors were collected from parents and their children. Our findings support the utility of the Bland-Altman Method and Reduced Major Axis regression in comparing measurement methods. Results indicated stronger agreement between parent-reported and directly measured child language, and poorer agreement between measures of parenting behaviors. Child age was associated with difference scores for child language; however the direction varied for each cohort. Parents who rated their child’s temperament as more difficult tended to report lower language scores on the parent questionnaire, compared to the directly measured scores. Older parents tended to report lower parenting responsiveness on the parent questionnaire, compared to directly measured scores. Finally, speaking a language other than English was associated with less responsive parenting behaviors on the

  18. Realizing the American Dream: A Parent Education Program Designed to Increase Latino Family Engagement in Children's Education

    Science.gov (United States)

    Walker, Joan M. T.

    2016-01-01

    Grounded in Hoover-Dempsey and Sandler's parent involvement process model, the Realizing the American Dream (RAD) parent education program targets Latino parents' involvement beliefs and knowledge to enhance their involvement behaviors. Comparison of more than 2,000 parents' self-reported beliefs, knowledge, and behavior before and after RAD…

  19. The Role of Food Parenting Skills and the Home Food Environment in Children's Weight Gain and Obesity.

    Science.gov (United States)

    Gerards, S M P L; Kremers, S P J

    2015-03-01

    This paper presents an overview to provide readers with an update on the literature about the relation between parental influences (general parenting and food parenting practices) and children's weight-related outcomes. It first summarizes the evidence regarding the role of food parenting practices in shaping and maintaining children's nutritional and weight status. It then describes empirical evidence on the relation between general parenting and children's weight status. This evidence is less convincing, possibly because general parenting has a different, more distal role in influencing child behavior than parenting practices. General parenting may moderate the impact of food parenting practices on children's nutrition behaviors. Finally, we discuss studies on interventions targeting childhood overweight and obesity. There is no consensus on the optimal intervention targets (i.e., general parenting and/or food parenting practices). Based on the overview, we offer suggestions for future research.

  20. Impact of Caregiving for a Child With Cancer on Parental Health Behaviors, Relationship Quality, and Spiritual Faith: Do Lone Parents Fare Worse?

    Science.gov (United States)

    Wiener, Lori; Viola, Adrienne; Kearney, Julia; Mullins, Larry L; Sherman-Bien, Sandra; Zadeh, Sima; Farkas-Patenaude, Andrea; Pao, Maryland

    2016-09-01

    Caregiving stress has been associated with changes in the psychological and physical health of parents of children with cancer, including both partnered and single parents. While parents who indicate "single" on a demographic checklist are typically designated as single parents, a parent can be legally single and still have considerable support caring for an ill child. Correspondingly, an individual can be married/partnered and feel alone when caring for a child with serious illness. In the current study, we report the results from our exploratory analyses of parent self-reports of behavior changes during their child's treatment. Parents (N = 263) of children diagnosed with cancer were enrolled at 10 cancer centers. Parents reported significant worsening of all their own health behaviors surveyed, including poorer diet and nutrition, decreased physical activity, and less time spent engaged in enjoyable activities 6 to 18 months following their child's diagnosis. More partnered parents found support from friends increased or stayed the same since their child's diagnosis, whereas a higher proportion of lone parents reported relationships with friends getting worse. More lone parents reported that the quality of their relationship with the ill child's siblings had gotten worse since their child's diagnosis. Spiritual faith increased for all parents. © 2015 by Association of Pediatric Hematology/Oncology Nurses.

  1. Children of Parents With Serious Mental Illness

    DEFF Research Database (Denmark)

    Ranning, Anne; Munk Laursen, Thomas; Thorup, Anne

    2016-01-01

    Objective To provide an overview of living arrangements during childhood for children of parents with schizophrenia, bipolar disorder, and depression. Method Information was obtained from Danish registers on children's addresses and used to calculate the proportion living in different household...... living arrangements. The study was conducted as a prospective, register-based cohort study covering all children in the entire Danish population born after 1982 (N = 1,823,625) and their parents with a diagnosis of schizophrenia, bipolar disorder, depression, or none of these disorders. Regression...... with a single mother with SMI. Conjugal families were dissolved at higher rates if a parent had SMI, especially if the mother (incidence rate ratio 2.98; 95% CI 2.80–3.17) or the father (incidence rate ratio 2.60; 95% CI 2.47–2.74) had schizophrenia. Risks for family dissolution varied greatly with parents...

  2. Estimating the number of children exposed to parental psychiatric disorders through a national health survey

    Directory of Open Access Journals (Sweden)

    Padoin Cintia V

    2009-02-01

    Full Text Available Abstract Objective Children whose parents have psychiatric disorders experience an increased risk of developing psychiatric disorders, and have higher rates of developmental problems and mortality. Assessing the size of this population is important for planning of preventive strategies which target these children. Methods National survey data (CCHS 1.2 was used to estimate the number of children exposed to parental psychiatric disorders. Disorders were diagnosed using the World Psychiatric Health Composite International Diagnostic Interview (WMH-CIDI (12 month prevalence. Data on the number of children below 12 years of age in the home, and the relationship of the respondents with the children, was used to estimate exposure. Parent-child relations were identified, as was single parenthood. Using a design-based analysis, the number of children exposed to parental psychiatric disorders was calculated. Results Almost 570,000 children under 12 live in households where the survey respondent met criteria for one or more mood, anxiety or substance use disorders in the previous 12 months, corresponding to 12.1% of Canadian children under the age of 12. Almost 3/4 of these children have parents that report receiving no mental health care in the 12 months preceding the survey. For 17% of all Canadian children under age 12, the individual experiencing a psychiatric disorder is the only parent in the household. Conclusion The high number of children exposed causes major concern and has important implications. Although these children will not necessarily experience adversities, they possess an elevated risk of accidents, mortality, and of developing psychiatric disorders. We expect these estimates will promote further research and stimulate discussion at both health policy and planning tables.

  3. Risk factors for suicide in offspring bereaved by sudden parental death from external causes.

    Science.gov (United States)

    Burrell, Lisa Victoria; Mehlum, Lars; Qin, Ping

    2017-11-01

    Parentally bereaved offspring have an increased suicide risk as a group, but the ability to identify specific individuals at risk on the basis of risk and protective factors is limited. The present study aimed to investigate to what degree different risk factors influence suicide risk in offspring bereaved by parental death from external causes. Based on Norwegian registers, individual-level data were retrieved for 375 parentally bereaved suicide cases and 7500 parentally bereaved gender- and age-matched living controls. Data were analysed with conditional logistic regression. Bereaved offspring with low social support, indicated by offspring's single status and repeated changes in marital status and residence, had a significantly increased suicide risk compared to bereaved offspring with high social support. Moreover, low socioeconomic status, having an immigration background, having lost both parents and loss due to suicide significantly increased suicide risk. Several variables relevant to bereavement outcome, such as coping mechanisms and the quality of the parent-offspring relationship are impossible to examine by utilizing population registers. Moreover, the availability of data did not enable the measurement of marital stability and residence stability across the entire lifespan for older individuals. Healthcare professionals should be aware of the additional risk posed by the identified risk factors and incorporate this knowledge into existing practice and risk assessment in order to identify individuals at risk and effectively target bereaved family and friends for prevention and intervention programs. Ideal follow-up for bereaved families should include a specific focus on mobilizing social support. Copyright © 2017 Elsevier B.V. All rights reserved.

  4. Parenting on Your Own. Circulars 1245-1258.

    Science.gov (United States)

    Hughes, Robert, Jr.; And Others

    These 14 circulars for single parents provide advice about several aspects of parenting. Contents discuss developing self-esteem, conquering loneliness, managing stress, obtaining social support, managing time, controlling finances, getting and using credit, developing job-readiness skills, searching for a job, disciplining children effectively,…

  5. The effects of nonsuicidal self-injury on parenting behaviors: a longitudinal analyses of the perspective of the parent.

    Science.gov (United States)

    Baetens, Imke; Claes, Laurence; Onghena, Patrick; Grietens, Hans; Van Leeuwen, Karla; Pieters, Ciska; Wiersema, Jan R; Griffith, James W

    2015-01-01

    The present study is the first to examine predictors and consequences of nonsuicidal self-injury (NSSI) in adolescence using parent-reported data in a longitudinal design. Across three time points, we examined the reciprocal effects of parent-reported parenting behaviors as they are related to adolescents' NSSI. The present study is a three-wave prospective study in a large sample of community adolescents and their parents. At time 1 (age 12), the sample consisted of 1396 adolescent reports and 1438 parent reports. Time 2 (age 13) included 827 adolescent and 936 parent reports. At time 3 (age 14), 754 adolescent and 790 parent reports were obtained. Engagement in NSSI (adolescent report) was determined by an affirmative response to the item 'Have you intentionally injured yourself (e.g., cut, burn, scratch) this year, without the intent to die?'. Parental awareness of NSSI at age 13 and 14 was examined using a single-item screening question. Parenting behaviors were examined by the parent versions of the Parental Behavior Scale. Results showed that although NSSI was reported by 10 % of the adolescents, only 3 % of the parents were aware of the NSSI behaviors of their children. Cross-lagged analyses showed a reciprocal relationship between NSSI and parenting behaviors over time. We found a significant effect of both positive parenting and controlling parenting on the presence of NSSI at time 2. But vice versa NSSI also has an effect on parenting behaviors over time. Results showed that NSSI at time 1 has an impact on controlling parenting behaviors, namely punishment at time 2. NSSI at time 2 showed an impact on parent's perception of positive parenting, parental rule setting, punishment and harsh punishment. The present study examined predictors and consequences of NSSI in a longitudinal design, and emphasized the importance of examining reciprocal interactions between NSSI and parenting behaviors. Furthermore, it is the first study to examine parent

  6. Family socioeconomic status and child executive functions: the roles of language, home environment, and single parenthood.

    Science.gov (United States)

    Sarsour, Khaled; Sheridan, Margaret; Jutte, Douglas; Nuru-Jeter, Amani; Hinshaw, Stephen; Boyce, W Thomas

    2011-01-01

    The association between family socioeconomic status (SES) and child executive functions is well-documented. However, few studies have examined the role of potential mediators and moderators. We studied the independent and interactive associations between family SES and single parenthood to predict child executive functions of inhibitory control, cognitive flexibility, and working memory and examined child expressive language abilities and family home environment as potential mediators of these associations. Sixty families from diverse SES backgrounds with a school-age target child (mean [SD] age = 9.9 [0.96] years) were evaluated. Child executive functioning was measured using a brief battery. The quality of the home environment was evaluated using the Home Observation for the Measurement of the Environment inventory. Family SES predicted the three child executive functions under study. Single parent and family SES were interactively associated with children's inhibitory control and cognitive flexibility; such that children from low SES families who were living with one parent performed less well on executive function tests than children from similarly low SES who were living with two parents. Parental responsivity, enrichment activities and family companionship mediated the association between family SES and child inhibitory control and working memory. This study demonstrates that family SES inequalities are associated with inequalities in home environments and with inequalities in child executive functions. The impact of these disparities as they unfold in the lives of typically developing children merits further investigation and understanding.

  7. Social class, parental education, and obesity prevalence in a study of six-year-old children in Germany.

    Science.gov (United States)

    Lamerz, A; Kuepper-Nybelen, J; Wehle, C; Bruning, N; Trost-Brinkhues, G; Brenner, H; Hebebrand, J; Herpertz-Dahlmann, B

    2005-04-01

    To assess the association between socioeconomic status (SES) and childhood obesity, and which factor in particular stands out in relation to obesity. When 2020 children attended their obligatory health exam prior to school entry in the City of Aachen, Germany, 1979 parents (97.9%) filled out a questionnaire on their child's weight development and on indicators of their family's SES in a cross-sectional survey. In addition, standardized measures of weight and height were taken. More detailed information on several different SES variables, such as parental education, occupation, income, family constellation, single parenthood, and the location and size of the family residence was obtained by personal interviews in a subsample of all native German speaking children with a BMI > or = 85th percentile, defined as cases (n = 146), and with a BMI between the 40th and 60th percentile, defined as controls (n = 221). The indicators of parental education were most strongly associated with children's obesity. There was a strong dose-response relationship between a composed index of social class and obesity. Children of the lowest social status had a more than three-fold risk to be obese than children of the highest social status in the screening population (OR: 3.29, CI: 1.92-5.63). The findings established a strong relationship between parental years of education and childhood obesity. Prevention and treatment programs should endeavor to better target undereducated parents and their young children at high risk.

  8. Single Parenthood Impact on Street Children in Ibadan Metropolis ...

    African Journals Online (AJOL)

    FIRST LADY

    According to local laws of different nations or regions, single parenting is a two way streets, the custodial parent could be either that mother or the father, because of the role nature have bestowed upon women, most times, mothers are given custody of the children. Single parenthood Impact on Street Children in Ibadan ...

  9. Children's Food and Beverage Promotion on Television to Parents.

    Science.gov (United States)

    Emond, Jennifer A; Smith, Marietta E; Mathur, Suman J; Sargent, James D; Gilbert-Diamond, Diane

    2015-12-01

    Nutritionally poor foods are heavily advertised to children on television. Whether those same products are also advertised to parents on television has not been systematically examined. This study is a content analysis of advertisements for children's packaged foods and beverages aired over US network, cable, and syndicated television for 1 year (2012 to 2013). The target audience of each advertisement was defined as children or parents based on advertisement content, where parent-directed advertisements included emotional appeals related to family bonding and love. Advertisement characteristics and patterns of airtime were compared across target audience, and the proportion of total airtime devoted to advertisements targeting parents was computed. Fifty-one children's food or beverage products were advertised over the study year, 25 (49%) of which were advertised directly to parents. Parent-directed advertisements more often featured nutrition and health messaging and an active lifestyle than child-directed advertisements, whereas child-directed advertisements more frequently highlighted fun and product taste. Over all products, 42.4% of total airtime was devoted to advertisements that targeted parents. The products with the most amount of airtime over the study year were ready-to-eat cereals, sugar-sweetened beverages, and children's yogurt, and the proportion of total advertisement airtime for those products devoted to parents was 24.4%, 72.8%, and 25.8%, respectively. Television advertisements for children's packaged foods and beverages frequently targeted parents with emotional appeals and messaging related to nutrition and health. Findings are of concern if exposure to such advertisements among parents may shape their beliefs about the appropriateness of nutritionally questionable children's foods and beverages. Copyright © 2015 by the American Academy of Pediatrics.

  10. For Parents Particularly. The Critical Years: Ages 10-12.

    Science.gov (United States)

    O'Brien, Shirley J.

    1990-01-01

    Provides advice to single parents on ways to help their preadolescent children get in touch with their feelings and prepare for positive life experiences. The advice is highlighted with a case study. (BB)

  11. Parents of children with enduring epilepsy: predictors of parenting stress and parenting.

    NARCIS (Netherlands)

    Rodenburg, R.J.T.; Meijer, A.M.; Dekovic, M.; Aldenkamp, A.P.

    2007-01-01

    OBJECTIVE: The goals of the work described here were (1) to predict parenting stress and parenting from stressors, resources, and parental coping behaviors in parents of children with epilepsy, and (2) to determine whether parenting stress mediates the effects of these predictors on parenting.

  12. Parents of children with enduring epilepsy: predictors of parenting stress and parenting

    NARCIS (Netherlands)

    Rodenburg, R.; Meijer, A.M.; Dekovic, M.; Aldenkamp, A.P.

    2007-01-01

    Objective: The goals of the work described here were (1) to predict parenting stress and parenting from stressors, resources, and parental coping behaviors in parents of children with epilepsy, and (2) to determine whether parenting stress mediates the effects of these predictors on parenting.

  13. Parent Stress and Its Relation to Parent Perceptions of Communication following Parent-Coached Language Intervention

    Science.gov (United States)

    Smith, Ashlyn L.; Romski, Mary Ann; Sevcik, Rose A.; Adamson, Lauren B.; Bakeman, Roger

    2011-01-01

    The effects of a parent-coached language intervention on parent stress and its relation to parent perceptions of communication development were examined in 60 parents of toddlers with developmental delays. Results indicated that overall parent stress was not high prior to or following language intervention. Parents' perceptions about the severity…

  14. Review of parental activation interventions for parents of children with special health care needs.

    Science.gov (United States)

    Mirza, M; Krischer, A; Stolley, M; Magaña, S; Martin, M

    2018-02-27

    ingredients of interventions that specifically target enhancing parent skill sets relevant to areas of self-efficacy, confidence, and empowerment. Future studies must also adapt intervention and study design to recruit socioeconomically vulnerable families. © 2018 John Wiley & Sons Ltd.

  15. Positive parenting: a randomised controlled trial evaluation of the Parents Plus Adolescent Programme in schools.

    Science.gov (United States)

    Nitsch, Eileen; Hannon, Geraldine; Rickard, Eóin; Houghton, Sharon; Sharry, John

    2015-01-01

    The aim of this study was to evaluate the Parents Plus Adolescents Programme (PPAP)-a parent training course specifically targeting parents of young adolescents (aged 11-16 years)-when delivered as a preventative programme in community school settings. A sample of 126 parents (mean age of children = 12.34 years; range = 10-16 years) were randomly assigned to either a treatment (PPAP; n = 82) or a waiting-list control condition (WC; n = 44). Analyses are based on a study-completer sample post-treatment (n = 109 parents: PPAP n = 70; WC n = 39) and sample at 6 month follow up (n = 42 parents). Both post-treatment (between groups) and 6-month follow-up comparisons of study completers (within PPAP group) revealed significant positive effects of the parenting intervention with respect to adolescent behaviour problems and parenting stress. The post treatment comparisons demonstrated large effect sizes on global measures of child difficulties (partial eta squared = 0.15) and self-reported parent stress (partial eta squared = 0.22); there was a moderate effect size on the self-reported parent satisfaction (partial eta squared = 0.13). This study provides preliminary evidence that PPAP may be an effective model of parent-training implemented in a community-based setting. The strengths and limitations of the study are discussed.

  16. Dual targeting of peroxisomal proteins

    Directory of Open Access Journals (Sweden)

    Julia eAst

    2013-10-01

    Full Text Available Cellular compartmentalization into organelles serves to separate biological processes within the environment of a single cell. While some metabolic reactions are specific to a single organelle, others occur in more than one cellular compartment. Specific targeting of proteins to compartments inside of eukaryotic cells is mediated by defined sequence motifs. To achieve multiple targeting to different compartments cells use a variety of strategies. Here, we focus on mechanisms leading to dual targeting of peroxisomal proteins. In many instances, isoforms of peroxisomal proteins with distinct intracellular localization are encoded by separate genes. But also single genes can give rise to differentially localized proteins. Different isoforms can be generated by use of alternative transcriptional start sites, by differential splicing or ribosomal read-through of stop codons. In all these cases different peptide variants are produced, of which only one carries a peroxisomal targeting signal. Alternatively, peroxisomal proteins contain additional signals that compete for intracellular targeting. Dual localization of proteins residing in both the cytoplasm and in peroxisomes may also result from use of inefficient targeting signals. The recent observation that some bona fide cytoplasmic enzymes were also found in peroxisomes indicates that dual targeting of proteins to both the cytoplasm and the peroxisome might be more widespread. Although current knowledge of proteins exhibiting only partial peroxisomal targeting is far from being complete, we speculate that the metabolic capacity of peroxisomes might be larger than previously assumed.

  17. Observed child and parent toothbrushing behaviors and child oral health

    Science.gov (United States)

    COLLETT, BRENT R.; HUEBNER, COLLEEN E.; SEMINARIO, ANA LUCIA; WALLACE, ERIN; GRAY, KRISTEN E.; SPELTZ, MATTHEW L.

    2018-01-01

    Background Parent-led toothbrushing effectively reduces early childhood caries. Research on the strategies that parents use to promote this behavior is, however, lacking. Aim To examine associations between parent–child toothbrushing interactions and child oral health using a newly developed measure, the Toothbrushing Observation System (TBOS). Design One hundred children ages 18–60 months and their parents were video-recorded during toothbrushing interactions. Using these recordings, six raters coded parent and child behaviors and the duration of toothbrushing. We examined the reliability of the coding system and associations between observed parent and child behaviors and three indices of oral health: caries, gingival health, and history of dental procedures requiring general anesthesia. Results Reliabilities were moderate to strong for TBOS child and parent scores. Parent TBOS scores and longer duration of parent-led toothbrushing were associated with fewer decayed, missing or filled tooth surfaces and lower incidence of gingivitis and procedures requiring general anesthesia. Associations between child TBOS scores and dental outcomes were modest, suggesting the relative importance of parent versus child behaviors at this early age. Conclusions Parents’ child behavior management skills and the duration of parent-led toothbrushing were associated with better child oral health. These findings suggest that parenting skills are an important target for future behavioral oral health interventions. PMID:26148197

  18. Muenster Parental Programme empowers parents in communicating with their infant with hearing loss.

    Science.gov (United States)

    Glanemann, Reinhild; Reichmuth, Karen; Matulat, Peter; Zehnhoff-Dinnesen, Antoinette Am

    2013-12-01

    With the implementation of the Universal Newborn Hearing Screening (UNHS), the age of diagnosis of children with hearing loss (HL) has been steadily declining in the past years. Consequently, there is a need for early educational intervention methods that are suitable for infants at the preverbal level. To meet this need we have developed and evaluated the Muenster Parental Programme (MPP), a responsive parenting programme for parents of children with HL aged 3-18 months. It aims at enhancing the parents' communicative skills towards their child. The MPP is introduced following confirmation of a HL. Flanked by two individual counselling sessions, the programme comprises six group sessions and two single training sessions with video feedback. The focus of the programme lies in enhancing parents' responsive behaviour and in reducing inappropriate initiative behaviour. The present study involved 29 parents of 24 children aged 6.6 (mean, range: 3-12) months at the outset of the MPP. The children's degree of HL ranged from moderate to profound. Parents of children with unilateral HL and/or risk for an additional developmental delay were included. The prospective study compared parent communication skills of a trained (N = 15) versus a control group (N = 14) before and after the MPP. For this purpose, instances of responsive behaviour to the signals of the child and total time of initiative behaviour within a 4-min video-sample were measured before and after completion of the study in both groups. Trained parents could enhance their responsiveness to vocal and preverbal signals of the child (Wilcoxon test, p = .002) and also their responsiveness to non-verbal signals (Wilcoxon test, p communication skills. The MPP constitutes the first evaluated group concept for parents of infants with HL in the German-speaking countries and equally meets the needs of parents and professionals. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  19. Parenting practices were associated with children's TV viewing among overweight and obese children

    Science.gov (United States)

    An expert panel recommended that TV reduction should be a component in obesity treatment programs. Parents are an important social influence on children and could be a target for interventions. Valid measures of TV-parenting practices (PP) are needed to understand parental influences on children's T...

  20. Perspectives on Stress, Parenting, and Children's Obesity-Related Behaviors in Black Families

    Science.gov (United States)

    Parks, Elizabeth P.; Kazak, Anne; Kumanyika, Shiriki; Lewis, Lisa; Barg, Frances K.

    2016-01-01

    Objective: In an effort to develop targets for childhood obesity interventions in non-Hispanic-Black (Black) families, this study examined parental perceptions of stress and identified potential links among parental stress and children's eating patterns, physical activity, and screen-time. Method: Thirty-three self-identified Black parents or…

  1. SINGLE–PARENT FAMILIES–SOCIAL STATUS, NEEDS AND CHALLENGES

    Directory of Open Access Journals (Sweden)

    Anita Burgund

    2013-02-01

    Full Text Available There is no doubt that pluralization of society and societal conditions have an impact on the modern family, its role and structure. Changes in the hierarchy of societal values and attitude of society towards family lead to significant changes in its structure and way of functioning. The image of the family consisting of father, mother and children has more often been replaced by the image of families with one parent and children. There are multiple causes of single-parent families (single parenthood such as death, divorce, abandonment of the family by one of the parents, etc. The positions and attitude of society towards single-parent families is different and it’s actually depending on the cause of their occurrence. The aim of this paper was to identify basic needs and challenges of single-parent families in Serbia. For this purpose, we have organized three focus groups with 18 single mothers (six in each focus group. The results are presented in the context of creating new policies for improving the position of single-parent families in Serbia.

  2. Stress in Parents of a Child with Hemifacial Microsomia: The Role of Child Characteristics and Parental Coping Strategies.

    Science.gov (United States)

    Ongkosuwito, Edwin; van der Vlies, Lieneke; Kraaij, Vivian; Garnefski, Nadia; van Neck, Han; Kuijpers-Jagtman, Anne Marie; Hovius, Steven

    2018-01-01

    Objective Examine stress levels of parents of children with hemifacial microsomia (HFM) and the relationship of parental stress to child characteristics and cognitive coping strategies. Design Prospective cross-sectional study. Participants and Setting Parents with a child (age 3-19 years) with HFM (N = 31) were recruited through the Department of Orthodontics and the Craniofacial Center, Sophia-Erasmus Medical Center, Rotterdam, The Netherlands. Intervention and Outcome Measures The adapted and shortened Dutch version of the parental stress index (NOSI-K) was used to measure parental stress, and the cognitive emotion-regulation questionnaire was used to measure cognitive coping strategies. Pearson correlations and a multiple regression analysis were performed. Results The hierarchical multiple regression analysis showed associations between increased parental stress and learning difficulties and use of acceptance as a coping strategy. This suggests that problems other than the characteristic visual appearance of the child's face in HFM have a greater influence on parental stress. Conclusions Learning difficulties of the child with HFM and parental acceptance affect stress in parents with a child with HFM the most and are important in the search for a targeted tailoring of intervention for parents with high levels of parental stress.

  3. Parenting and juvenile delinquency

    OpenAIRE

    Hoeve, M.

    2008-01-01

    Juvenile delinquency is a noteworthy problem. This thesis addressed the association between parenting and juvenile delinquency by analyzing the concepts of parenting adopted in family research in relation to criminological concepts and measures of delinquent behavior. Four studies were conducted. The first study addressed a meta-analysis on parenting characteristics and styles in relation to delinquency. In this meta-analysis, previous manuscripts were systematically analyzed, computing mean ...

  4. Parenting style, parenting stress, and children's health-related behaviors.

    Science.gov (United States)

    Park, Hyunjeong; Walton-Moss, Benita

    2012-07-01

    Parental guidance is critical to the development of children's health-related behaviors. The purpose of this study was to look at the relationship between parenting factors, including parenting style and parenting stress, and children's health-related behaviors. In this descriptive, correlational study, 284 parents of preschool children were interviewed using the Child Rearing Questionnaire and the Korean Parenting Stress Index-Short Form. Parent distress, authoritative and permissive parenting styles, family income, and mother's education were significantly associated with children's health-related behaviors. These findings suggest that higher levels of warmth, characteristics of both parenting styles, may be a critical factor in the development of health-related behaviors.

  5. Parents' Perspectives on Parental Notification of College Students' Alcohol Use

    Science.gov (United States)

    Cosden, Merith; Hughes, Jennifer B.

    2012-01-01

    Although many colleges and universities use "parental notification" to inform parents of students' alcohol use, the impact of this intervention on student and parent behavior is unclear. Surveys were obtained from 326 parents of university undergraduates, 56 of whom had received a notification. Parent responses to the notification were…

  6. Parenting Styles and Conceptions of Parental Authority during Adolescence.

    Science.gov (United States)

    Smetana, Judith G.

    1995-01-01

    Reports of parenting styles were assessed in 100 mostly white, middle-class, 6th, 8th, and 10th graders and their parents. Adolescents viewed their parents as more permissive and more authoritarian than parents viewed themselves, whereas parents viewed themselves as more authoritative than did adolescents. Differences were primarily over the…

  7. Personality and parenting style in parents of adolescents

    NARCIS (Netherlands)

    Huver, R.M.E.; Otten, R.; Vries, H. de; Engels, R.C.M.E.

    2010-01-01

    Since parental personality traits are assumed to play a role in parenting behaviors, the current study examined the relation between parental personality and parenting style among 688 Dutch parents of adolescents in the SMILE study. The study assessed Big Five personality traits and derived

  8. Burglar Target Selection

    Science.gov (United States)

    Townsley, Michael; Bernasco, Wim; Ruiter, Stijn; Johnson, Shane D.; White, Gentry; Baum, Scott

    2015-01-01

    Objectives: This study builds on research undertaken by Bernasco and Nieuwbeerta and explores the generalizability of a theoretically derived offender target selection model in three cross-national study regions. Methods: Taking a discrete spatial choice approach, we estimate the impact of both environment- and offender-level factors on residential burglary placement in the Netherlands, the United Kingdom, and Australia. Combining cleared burglary data from all study regions in a single statistical model, we make statistical comparisons between environments. Results: In all three study regions, the likelihood an offender selects an area for burglary is positively influenced by proximity to their home, the proportion of easily accessible targets, and the total number of targets available. Furthermore, in two of the three study regions, juvenile offenders under the legal driving age are significantly more influenced by target proximity than adult offenders. Post hoc tests indicate the magnitudes of these impacts vary significantly between study regions. Conclusions: While burglary target selection strategies are consistent with opportunity-based explanations of offending, the impact of environmental context is significant. As such, the approach undertaken in combining observations from multiple study regions may aid criminology scholars in assessing the generalizability of observed findings across multiple environments. PMID:25866418

  9. [New parenting education in maternal child nursing].

    Science.gov (United States)

    Chen, Jih-Yuan

    2009-12-01

    Taiwan society is today typified by low birth rates amongst Taiwanese and a rising number of children born to immigrant and trans-cultural marriage families. Unhealthy behavior and anxiety on the part of pregnant women increase postpartum depression and complications and negatively affect neonatal heath. Such may further negatively impact upon romantic feelings between the new parents and the nascent parent-child relationship. New parenting education is a proactive and innovative strategy that may be used to improve maternal and child health. Therefore, it is worthy to explore how best to achieve cost-effective education interventions. First, the importance of new parenting education and its influence factors must be understood. Factors of women's health and nursing responsibilities potentially addressed by new parenting education include pregnancy complications, fetal death and malformation, accidents and traumas during childhood and adolescence, childhood obesity, and pediatric health-care delivery systems. It is the responsibility of nursing professionals to collect and interpret information on health promotion, disease prevention and childcare in cooperation with other disciplines. Nurses are also responsible to participate in family education and services that target new parents. Therefore, nursing professionals participate in planning and intervention actions related to health promotion, develop support group and counseling centers, collect and organize relevant information, and develop family education and health promotion models. Achieving preventive health service goals while maintaining family competencies and empowerment is an essential aspect of the parenthood mission and vision.

  10. Motives for Transfers from Parents to Children

    DEFF Research Database (Denmark)

    Kolodziejczyk, Christophe; Leth-Petersen, Søren

    There are good theoretical reasons why transfers from parents are likely to be important around the time of the first home purchase. Transactions costs associated with trading houses make people with increasing income paths prefer to buy a house that is more expensive than what matches their curr......There are good theoretical reasons why transfers from parents are likely to be important around the time of the first home purchase. Transactions costs associated with trading houses make people with increasing income paths prefer to buy a house that is more expensive than what matches...... suggested that transfers from parents to children are significant exactly around the time where children buy their first home. Using a panel data set issued from Danish administrative registers with information about wealth of a sample of first-time homeowners and their parents, we document that child...... and parent resources, house value as well as financial resources are correlated. We then go on to test if there are direct parental transfers targeted to the purchase of the house, and in case of an unemployment spell during the years after the purchase where children typically hold little liquid assets. We...

  11. Using the Dual-Target Cost to Explore the Nature of Search Target Representations

    Science.gov (United States)

    Stroud, Michael J.; Menneer, Tamaryn; Cave, Kyle R.; Donnelly, Nick

    2012-01-01

    Eye movements were monitored to examine search efficiency and infer how color is mentally represented to guide search for multiple targets. Observers located a single color target very efficiently by fixating colors similar to the target. However, simultaneous search for 2 colors produced a dual-target cost. In addition, as the similarity between…

  12. The Association of Harsh Parenting, Parent-Child Communication, and Parental Alcohol Use With Male Alcohol Use Into Emerging Adulthood.

    Science.gov (United States)

    Diggs, Olivia N; Neppl, Tricia K; Jeon, Shinyoung; Lohman, Brenda J

    2017-12-01

    This study investigates the association between mother and father harsh parenting, and parent-child communication, and parental alcohol use on males' alcohol use from early adolescence into emerging adulthood. Data come from the Iowa Youth and Families Project, a prospective 28-year longitudinal study of rural Midwestern youth and their families. Mother and father harsh parenting, parent-child communication, and alcohol use were assessed at time 1 when males were in early adolescence (13 years old, n = 215). Target male alcohol use was assessed at time 2 during late adolescence (18 and 19 years old, n = 206, 96% follow-up rate), and at time 3 in emerging adulthood (23 and 25 years old, n = 197, 92% follow-up rate). Results obtained from structural equation modeling using Mplus, version 7, statistical software indicated that father harsh parenting in early adolescence was directly associated with alcohol use in emerging adulthood. Mother communication was negatively associated while father alcohol use was positively associated with adolescent alcohol use in late adolescence and emerging adulthood. Alcohol use in late adolescence was significantly related to alcohol use in emerging adulthood. This study offers unique insights into how mother- and father-son dyads differ in communication and parenting styles, as well as how these associations influence adolescent male alcohol use continuing into emerging adulthood. Multiple informants utilized in the current study provide a more complex understanding of how each parent uniquely contributes to the role of their adolescent's alcohol use in late adolescence into emerging adulthood. Copyright © 2017 Society for Adolescent Health and Medicine. Published by Elsevier Inc. All rights reserved.

  13. Energy and traffic light labelling have no impact on parent and child fast food selection.

    Science.gov (United States)

    Dodds, Pennie; Wolfenden, Luke; Chapman, Kathy; Wellard, Lyndal; Hughes, Clare; Wiggers, John

    2013-10-25

    Labelling of food from fast food restaurants at point-of-purchase has been suggested as one strategy to reduce population energy consumption and contribute to reductions in obesity prevalence. The aim of this study was to examine the effects of energy and single traffic light labelling systems on the energy content of child and adult intended food purchases. The study employed a randomised controlled trial design. English speaking parents of children aged between three and 12 years were recruited from an existing research cohort. Participants were mailed one of three hypothetical fast food menus. Menus differed in their labelling technique- either energy labels, single traffic light labels, or a no-label control. Participants then completed a telephone survey which assessed intended food purchases for both adult and child. The primary trial outcome was total energy of intended food purchase. A total of 329 participants completed the follow-up telephone interview. Eighty-two percent of the energy labelling group and 96% of the single traffic light labelling group reported noticing labelling information on their menu. There were no significant differences in total energy of intended purchases of parents, or intended purchases made by parents for children, between the menu labelling groups, or between menu labelling groups by socio-demographic subgroups. This study provided no evidence to suggest that energy labelling or single traffic light labelling alone were effective in reducing the energy of fast food items selected from hypothetical fast food menus for purchase. Additional complementary public health initiatives promoting the consumption of healthier foods identified by labelling, and which target other key drivers of menu item selection in this setting may be required. Copyright © 2013. Published by Elsevier Ltd.

  14. Impact of Childhood Family Disruption on Young Adults' Relationships with Parents.

    Science.gov (United States)

    Aquilino, William S.

    1994-01-01

    Explored implications of childhood family disruption for parent-adult child relations in sample of 4,516 young adults. Among young adults raised in single-parent families, relationships with custodial mothers and custodial fathers remained quite positive into early adulthood. Becoming noncustodial parent resulted in severe deterioration of…

  15. How Attendance and Quality of Participation Affect Treatment Response to Parent Management Training

    Science.gov (United States)

    Nix, Robert L.; Bierman, Karen L.; McMahon, Robert J.

    2009-01-01

    This study examined whether attendance and quality of participation in parent management training predicted treatment response. Data were from 445 parents (55% minority, 62% single; almost all of low socioeconomic status) who had 1st-grade children with severe conduct problems. Quality of participation in weekly parent groups was based on group…

  16. Parents' "Perezhivanie" Supports Children's Development of Emotion Regulation: A Holistic View

    Science.gov (United States)

    Chen, Feiyan

    2015-01-01

    Parents play an influential role in children's emotional development. Numerous quantitative studies have examined the correlations between a "single" dimension of parents' emotion socialisation practices (e.g. parental emotion expression or attitudes) and children's emotional development. However, little attention has been paid to a…

  17. Analysis of the Psychometric Properties of a Parental Alienation Scale

    Directory of Open Access Journals (Sweden)

    Paula Inez Cunha Gomide

    Full Text Available Abstract The development of forensic evaluation scales is fundamental. This study's purpose was to explore the psychometric properties of a parental alienation scale. Forensic technicians completed 193 scales concerning parents involved in a lawsuit: 48 families with at least one parent indicated as the alienator (group A and 48 families with no parental alienation claim (group B. The scale consisted of five categories and 69 items: denying access to the child; derogatory comparisons; emotional manipulation; behavior of parent and child during assessment. The results show Cronbach's alpha = .965 and split-half = .745; KMO = .884 and Bartlett's sphericity test ( p < .001. Concurrent criterion validity applied to data showed that the scale is able to distinguish between the alienator and target parent. The results showed significant and consistent standards in the instrument's psychometric characteristics.

  18. What matters most - what parents model or what parents eat?

    Science.gov (United States)

    Vaughn, Amber E; Martin, Chantel L; Ward, Dianne S

    2018-03-28

    Parents have a strong influence on their children's eating habits; however, researchers struggle to identify which food parenting practices to recommend. This study examined the influence of parents modeling of healthy eating ("parent role modeling") and parents' actual food intake ("parent dietary intake") on child diet quality, and explored whether these practices work together to influence children's diets. Baseline data from a larger intervention trial were used for this analysis. The sample included parents of preschool-age children from households with at least one overweight parent. The Comprehensive Feeding Practices Questionnaire was used to assess parent modeling of healthy eating ("healthy modeling"). Three days of dietary recalls were used to collect parents' report of their own intake and their children's intake (excluding food at child care). Associations between parent healthy modeling and parent intake of healthy and unhealthy foods were explored using Pearson correlations. Associations between parent healthy modeling and parent Healthy Eating Index (HEI) score on child HEI score were examined with linear regression. Additionally, the interaction between parent healthy modeling and HEI score on child HEI score was tested. Parent healthy modeling was significantly correlated with parent intake of healthy foodsLinear regression showed a significant association between parent modeling and child HEI score, even after controlling for parent diet (β = 3.08, SE = 0.87, p parents had high parent healthy modeling scores had higher HEI scores (mean = 61.5 ± 10.4) regardless of parent HEI score. We did not find evidence that parent healthy modeling and diet quality interact to influence child diet quality. Parents' healthy modeling is an important practice in influencing children's diet quality, possibly more so than the quality of parents' diets. Copyright © 2018. Published by Elsevier Ltd.

  19. Evaluation of the Prevalence of Drug Abuse and Smoking in Parents of Children with Attention Deficit Hyperactivity Disorder

    OpenAIRE

    Parvaresh, Nooshin; Mazhari, Shahrzad; Mohamadi, Neda; Mohamadi, Najmeh

    2016-01-01

    Background Attention deficit hyperactivity disorder (ADHD) affects 5% of children. In addition to pharmacotherapy, non-drug treatments such as appropriate parenting are also very important in the treatment of these children. Diagnosis and treatment of parents with psychiatric disorders and substance abuse and evaluation of the frequency of these disorders in parents is critical. Methods In this case-control study, 200 parents were studied. The target population included parents of 7 to 12 yea...

  20. Parenting and adolescents' accuracy in perceiving parental values.

    Science.gov (United States)

    Knafo, Ariel; Schwartz, Shalom H

    2003-01-01

    What determines adolescents' accuracy in perceiving parental values? The current study examined potential predictors including parental value communication, family value agreement, and parenting styles. In the study, 547 Israeli adolescents (aged 16 to 18) of diverse socioeconomic backgrounds participated with their parents. Adolescents reported the values they perceive their parents want them to hold. Parents reported their socialization values. Accuracy in perceiving parents' overall value system correlated positively with parents' actual and perceived value agreement and perceived parental warmth and responsiveness, but negatively with perceived value conflict, indifferent parenting, and autocratic parenting in all gender compositions of parent-child dyads. Other associations varied by dyad type. Findings were similar for predicting accuracy in perceiving two specific values: tradition and hedonism. The article discusses implications for the processes that underlie accurate perception, gender differences, and other potential influences on accuracy in value perception.