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Sample records for single mothers living

  1. Living Arrangements among Single Mothers in India

    Directory of Open Access Journals (Sweden)

    Swain, Pushpanjali

    2005-01-01

    Full Text Available EnglishA large proportion of two parent households transition into single parenthouseholds upon marital disruption. Single parent households, especially femaleheaded households face an increase in the risk of poverty. This study focuses onwhether widow status in India influences the likelihood of household headshipin India. This study is based on the most recent National Family Health Survey1998-1999, in India. The population of interest includes all mothers aged 15 to49 years, without a spouse, living with one or more of their own children under18 years of age. We find that being a widow increases the likelihood of being ahousehold head compared to the likelihood of household headship among thedivorced and the separated in India. This study provides some evidence on thecontinuing social discrimination against widows in India. The implication of thefindings are discussed.FrenchUne grande proportion des ménages biparentaux se transforme en ménagesmonoparentaux après une séparation maritale. Les ménages monoparentaux,spécialement ceux qui sont dirigés par une femme, font face à un danger plusélevé de sombrer dans la pauvreté. Cette étude examine à quel point le faitd’être veuve influence la probabilité de se retrouver à la tête d’un ménage enInde. Cette étude est basée sur le recensement sur la santé des familles de 1998-1999, le plus récent en Inde. La population d’intérêt comprend toutes les mèresâgées de 15 à 49 ans, sans époux et qui vivent avec au moins un de leurs propresenfants de moins de 18 ans. Nous avons trouvé qu’en Inde, le fait d’être veuveaugmente la probabilité qu’une femme se retrouve à la tête de son ménage encomparaison aux femmes divorcées ou séparées. Cette étude avance despreuves de cette discrimination sociale continue en Inde envers les veuves. Lesimplications de ces constatations sont discutées.

  2. Everyday life and occupational deprivation in single migrant mothers living in Denmark

    DEFF Research Database (Denmark)

    Kielsgaard, Kamilla; Kristensen, Hanne Kaae; Nielsen, Dorthe Susanne

    2018-01-01

    ABSTRACT Background: Socio-economically disadvantaged single migrant mothers in Denmark risk poor health and social marginalisation, which affects participation in relevant occupations. Literature focusing on occupational deprivation in vulnerable groups such as migrants is sparse. Aim/objectives......ABSTRACT Background: Socio-economically disadvantaged single migrant mothers in Denmark risk poor health and social marginalisation, which affects participation in relevant occupations. Literature focusing on occupational deprivation in vulnerable groups such as migrants is sparse. Aim....../objectives: To explore how single migrant mothers experience their living conditions and the significance of those conditions on their exclusion from everyday life occupations. Material and methods: In-depth interviews were used to find how occupational deprivation manifests in the everyday lives of three women. Based...... the women of participation in social and work-related occupations. The women handled these conditions by trying to create a well-functioning family life. Conclusions and significance: Experiences of occupational deprivation take place in interaction with cultural norms, social position and lack of social...

  3. Everyday life and occupational deprivation in single migrant mothers living in Denmark

    DEFF Research Database (Denmark)

    Kielsgaard, Kamilla; Kristensen, Hanne Kaae; Nielsen, Dorthe Susanne

    2018-01-01

    on Ricoeur’s theory of interpretation, data were analysed and the meaning structures, in the form of three themes, were identified. Results: The societal and individual conditions of women’s everyday lives interact in a complex interplay, where immigration, illness and divorce, in particular, deprive...... the women of participation in social and work-related occupations. The women handled these conditions by trying to create a well-functioning family life. Conclusions and significance: Experiences of occupational deprivation take place in interaction with cultural norms, social position and lack of social....../objectives: To explore how single migrant mothers experience their living conditions and the significance of those conditions on their exclusion from everyday life occupations. Material and methods: In-depth interviews were used to find how occupational deprivation manifests in the everyday lives of three women. Based...

  4. The Influence of Grandparents in Single-Mother Families

    Science.gov (United States)

    Dunifon, Rachel; Kowaleski-Jones, Lori

    2007-01-01

    This article examines whether children living with single mothers benefit when they also live with a grandparent, using data from the 1979 to 2002 waves of the National Longitudinal Survey of Youth merged mother-child file (N = 6,501). Results indicate that for White children, living with a single mother and a grandparent is associated with…

  5. Single Mothers and the Effects of Intensive Aid on the Quality of their Lives : the Examples of the Netherlands and Cyprus

    NARCIS (Netherlands)

    Anne van den Berg; Maria Michailidis

    2005-01-01

    The objective of this study is to illuminate the effects of intensive aid on the self-reliance and independence of single mothers, and to show what is and what can be done to improve the empowerment of this group. Firstly the paper focuses on the effects of intensive social aid on the self-reliance

  6. The Lived Experiences of Single Hispanic Mothers Raising Gang-Affiliated Male Youth Released from Texas Juvenile Justice Department State Facilities: A Phenomenological Study

    Science.gov (United States)

    Rodriguez-Almendarez, Ruby

    2013-01-01

    Purpose of the Study: The purpose of this transcendental phenomenological study (Moustakas, 1994) was to describe the experiences that single Hispanic mothers of gang-affiliated male juveniles face during their sons' reentry process after being released from a Texas Juvenile Justice Department state facility. Methods: After an extensive review of…

  7. Mothers, wives and changing lives.

    OpenAIRE

    Brown, Brian J.; Baker, Sally

    2011-01-01

    An evocative, theoretically rich study of women’s lives in mid-twentieth century Wales which shows how they laid the foundations for feminism in the present generation. Women have made a major contribution to Wales as it is today, but so far there have been few authors who have brought the story of their struggle to light. This book fills that gap in our knowledge. With historical examples and richly-detailed accounts from people reminiscing about their own and their families’ histories, t...

  8. Single Mother Parenting and Adolescent Psychopathology.

    Science.gov (United States)

    Daryanani, Issar; Hamilton, Jessica L; Abramson, Lyn Y; Alloy, Lauren B

    2016-10-01

    Children raised in single-mother families are at increased risk for psychopathology, but the mechanisms that help explain this relationship are understudied. In a community sample of diverse adolescents (N = 385, 52 % female, 48 % Caucasian) and their mothers, we hypothesized that single mothers would be more likely than cohabitating mothers to engage in negative parenting behaviors, which would predict adolescent psychopathology prospectively. Single mothers were more likely to engage in psychologically controlling behaviors, which predicted to their adolescent offspring experiencing higher rates of depressive symptoms and externalizing disorders. Girls were more susceptible to depressive symptoms via psychologically controlling parenting than boys in single-mother families. Further, single mothers were more likely to engage in rejecting parenting behaviors, which predicted to a higher prevalence of adolescent externalizing disorders. Surprisingly, rejection in single-mother families predicted to less severe anxiety symptoms in adolescents relative to two-parent families. It is likely that single mothers are not inherently inferior parents relative to cohabitating mothers; rather, their parenting practices are often compromised by a myriad of demands and stressors. Consistent with this postulate, low socioeconomic status was associated with single motherhood and negative parenting behaviors. Clinical implications and study limitations are discussed.

  9. Unsettled scripts: intimacy narratives of heterosexual single mothers

    OpenAIRE

    Morris, Charlotte

    2014-01-01

    Drawing on contemporary theories of intimacy, this study explores the intimacy narratives and practices of single mothers at a time of, it is argued, social and cultural change in terms of intimacy. Narrative interviews of twenty-four single mothers draw out layers of personal, social and cultural complexity in terms of understanding, experiencing and making choices about intimacy in their everyday lives. The concept of ‘intimacy scripts’ (developed from Simon and Gagnon, 1973) is deployed to...

  10. The Life Course of Children Born to Unmarried Mothers: Childhood Living Arrangements and Young Adult Outcomes.

    Science.gov (United States)

    Aquilino, William S.

    1996-01-01

    Explored living arrangements among children born to unmarried mothers and the impact of childhood living arrangements on the young adult's life course. Analyses showed that living arrangement patterns after birth to a single mother influenced the likelihood of high school completion, post secondary education, and other conditions. (RJM)

  11. Single Motherhood, Living Arrangements, and Time With Children in Japan.

    Science.gov (United States)

    Raymo, James M; Park, Hyunjoon; Iwasawa, Miho; Zhou, Yanfei

    2014-08-01

    The authors examined relationships between single parenthood and mothers' time with children in Japan. Using data from the 2011 National Survey of Households with Children (N = 1,926), they first demonstrate that time spent with children and the frequency of shared dinners are significantly lower for single mothers than for their married counterparts. For single mothers living alone, less time with children reflects long work hours and work-related stress. Single mothers coresiding with parents spend less time with children and eat dinner together less frequently than either married mothers or their unmarried counterparts not living with parents, net of (grand)parental support, work hours, income, and stress. The findings suggest that rising divorce rates and associated growth in single-mother families may have a detrimental impact on parents' time with children in Japan and that the relatively high prevalence of intergenerational coresidence among single mothers may do little to temper this impact.

  12. Undergraduate Single Mothers' Experiences in Postsecondary Education

    Science.gov (United States)

    Beeler, Sydney

    2016-01-01

    Using Astin's (1993) College Impact Model, this chapter explores the current literature as it relates to single mothers in undergraduate postsecondary education. The chapter looks at the ways that undergraduates who are single mothers are counter to the "ideal-student" norms. Policy and best-practice recommendations conclude the chapter.

  13. A Supportive Service to Single Mothers and Their Children

    Science.gov (United States)

    Heger, Donna Tubach

    1977-01-01

    Describes a supportive program for single, young mothers in need of low-cost housing, job-training or schooling, social services and day care. Most enrollees gained a saleable skill and made progress in personal growth and the development of independent living skills. Techniques included a team approach and use of short-term contracts. (BF)

  14. [Children of single mothers: health risks and environmental stress].

    Science.gov (United States)

    Scharte, M; Bolte, G

    2012-03-01

    In Germany the risk for relative poverty has increased profoundly during the last 15 years, especially among single parent families. As poverty is often associated with bad health we examined the physical and mental health as well as health-related behaviour, housing and environmental conditions in children with lone mothers versus children in couple families. In 3 cross-sectional surveys conducted during 2004-2007 in 3 cities and 3 rural areas in Bavaria data on 19 039 pre-school children (47% female) were collected. Health, behaviour and exposure assessment was based on parental reports. The 18 327 cases with complete information on family status were analysed. 10% of the children grew up with single mothers. Single mothers evaluated the general state of health of their children more often as moderate to very poor than couple parents (OR [95% CI]: male: 1.37 [1.07-1.77], female 1.77 [1.33-2.35]). Sons with single mothers were more often obese (1.44 [1.09-1.90]). They scored significantly higher in the SDQ total difficulties score (1.94 [1.44-2.62]), on the emotional problem scale (1.91 [1.40-2.59]) as well as on the hyperactivity scale (1.82 [1.35-2.47]) compared to boys from couple families. No difference was found in prosocial behaviour. Girls with single mothers revealed more often conduct problems 1.36 [1.02-1.81] compared to those from couple families. They also showed a significantly higher prevalence of asthma (2.06 [1.29-3.30]). Children living with their single mothers were less often members of sports clubs and the boys were less physical active. No difference was found regarding the consumption of fruits and vegetables. Single mother family was associated with children's environmental tobacco smoke exposure at home (2.03 [1.79-2.29]). Single mothers perceived higher environmental exposures to noise and air pollution, suffered more often from a lack of accessible green spaces in the neighbourhood and reported a higher traffic load on the residential

  15. Stress Level and Adversity Quotient among Single Working Mothers

    Directory of Open Access Journals (Sweden)

    Dianne Bautista Solis

    2015-12-01

    Full Text Available The study identified the profile of the single working mothers in terms of number of children, number of years as a single parent and reason for being a single parent; assessed the single mothers’ stress level and adversity quotient; determined the significant difference of stress level and adversity quotient of single mothers when grouped according to profile variables; determined the best predictor of stress level and adversity quotient. Moreover this research endeavoured to test significant relationship between the adversity quotient and stress level of single working mothers. Lastly, it proposed a stress management program for single working mothers for them to cope with their stress and adversities in life. The researcher employed quantitative method using standardized questionnaires namely Depression, Anxiety, Stress Scale (DASS and Adversity Response Profile (ARP. The respondents were twenty five (25 single working mothers of the students of Batangas State University. From the results, majority of the respondents have 3 children, widow and in early years as single parent; with a normal level of stress and an average adversity quotient.. There are no significant differences on the stress level and adversity quotient of the respondents when grouped according to profile variables. Finally, stress level has no significant effect on adversity quotient of single working mothers. From the findings, the researcher further recommends that the Office of Guidance and Counseling should update the student information database to determine students with a single working mother. The Parent-Teacher Association may form a single-parent subgroup for the single working mothers to be able to identify to other mothers with same situation. Moreover, the proposed stress management program may be reviewed and implemented by the Office of Guidance and Counseling in coordination with the Parent-Teacher Association of Batangas State University. Future researchers

  16. Single mothering as experienced by Burundian refugees in Australia: a qualitative inquiry.

    Science.gov (United States)

    Tsai, Lily P; Barr, Jennieffer A; Welch, Anthony

    2017-01-01

    Refugee mothers have fled from their homeland to escape persecutions with their children only to find other threats to their well-being in the new country. Building on previous research, it is known that being a new immigrant is challenging and requires adaptation. The adaptation process, known as acculturation, may not be successful leading to psychological distress. It is also known that a generation gap can occur when children acculturate faster than their parents. What was lacking was understanding about the experiences of single refugee mothers. Interpretative phenomenological study was undertaken to explore the lived experiences of eight Burundian refugee single mothers in Australia. Data were collected by in-depth interviews. Each interviews were transcribed and analyzed using thematic analysis. Findings revealed three themes. First theme 'Traditional mothering practices of Burundian culture' illustrated mothering strategies as practiced prior to their arrival in Australia including mothering with sufficient social support, strong position of parents, and regular use of physical disciplining. Second theme 'Challenges identified after arrival to new country' revealed that mothers felt their children acculturated faster than themselves which led to intergenerational gap. This has also led participants to live in a continuous dilemma, experiencing inner conflicts and struggles associated with their mothering practices, especially when mothers had arrived with a lack of knowledge relating to acceptable mothering practices in a new culture. Final theme, 'Reforming family life in Australia' highlighted the decisions made by single refugee mothers which is to embrace both new and original cultures, leading to successful acculturation. However, lack of appropriate knowledge of acceptable mothering practices led to involvement of legal authorities who threatening to remove children from the mother's care. This has led mothers feeling change of power from 'mother to

  17. African American Single Mothers Raising Sons: Implications for Family Therapy

    Science.gov (United States)

    Gantt, Ann L.; Greif, Geoffrey L.

    2009-01-01

    Being raised by a single mother is one factor that has been suggested as contributing to the plight of African American males. Yet few studies have focused specifically on African American single mothers' experiences with raising sons. This qualitative study explored the following questions: (1) What are the experiences of African American single…

  18. Single Molecule Spectroscopy: Single Live Cell

    Indian Academy of Sciences (India)

    kbhattacharjee

    Live Cell Imaging: Seeing inside a cell. • Cell: ~20,000 nm ~ 100 times bigger than focus. • Label different parts of a cell with fluorescent dye. • Cancer Cell: How different from a normal cell? cell. Space & time resolution ...

  19. "No Cosby Show": Single Black Mother Homes and How Black Men Build Romantic Relationships

    Science.gov (United States)

    Moore, Maia Niguel

    2016-01-01

    This qualitative study explored the lives of 11 Black men to better understand how Black men who were raised in single Black mother homes build romantic relationships with Black women. One focus group and a series of individual in-person interviews were conducted with the participants who ranged between 23 and 43 years of age. Participants were…

  20. Single Mothers by Choice and Inwedlock Mothers: Sex-Role Orientation, Locus of Control, and Social Support.

    Science.gov (United States)

    Holle, Kimberly Ann

    An emerging family constellation is the family headed by a "single mother by choice," a structure in which both single marital status and parental status are chosen. This study was conducted to determine whether single mothers by choice (N=12) differed significantly from inwedlock mothers (N=18) regarding their childbearing decisions.…

  1. Work life and mental wellbeing of single and non-single working mothers in Scandinavia.

    Science.gov (United States)

    Bull, Torill; Mittelmark, Maurice B

    2009-08-01

    This study examined levels and predictors of mental wellbeing in Scandinavian working single and non-single mothers, with a special focus on financial stress, job characteristics and work-family conflict. The European Social Survey Round 2 (2005) provided questionnaire data from 73 single and 432 non-single working mothers in Denmark, Sweden and Norway. Respondents answered questions about the outcome variables life satisfaction, happiness, and positive affect, and predictor variables financial stress, job characteristics, work-family conflict, and social support. Hierarchical multiple regression was used to assess the relationships between predictor variables and mental wellbeing outcomes. Single working mothers scored significantly lower on life satisfaction and happiness, but not on positive affect, than did non-single mothers. Financial stress was higher in the single mother group. There were no significant differences in levels of enriching or stressful job characteristics, or in levels of social support. While financial stress and work-family conflict were important predictors in both groups, the relationship between financial stress and wellbeing was far stronger in the single mother group. Confidant support was a significant predictor only in the single mother group, and social participation only in the non-single mothers group. This study suggests that the Scandinavian welfare democracies have not yet been successful in relieving the financial pressure experienced by single working mothers. Development of efficient financial support systems should be prioritized. Ways to reduce work-family conflict in both single and non-single mothers in Scandinavia should also be given increased attention.

  2. Nutritional status and health profile among single mothers in Kota ...

    African Journals Online (AJOL)

    ... subjects have normal BMI, 6.0% of them fall in the category of underweight, 39.0% were overweight and 22.0% were obese. It is suggested that future intervention programs should focus on preventing obesity problems related to chronic diseases. Keywords: nutritional status; single mother; health profile; Kelantan; obesity ...

  3. Living in the shadow of shame and stigma: Lived experience of mothers with deaf children

    Directory of Open Access Journals (Sweden)

    Hossein Ebrahimi

    2016-11-01

    Full Text Available Congenital deafness is one of the most common childhood disorders and every year many children are born with permanent hearing loss. The present study was carried out to understand the experience of mothers with deaf children. This study was conducted with a qualitative approach. The participants were 35 mothers of children with congenital deafness who were selected by purposive sampling. The data was collected through semi structured indepth interviews and analyzed using thematic analysis introduced by Braun and Clarke. Data analysis revealed 3 themes and 9 sub-themes including social stigma (prying eyes, pity aversion, feeling of discrimination, taunt from people, internalized stigma (feeling of inferiority, feeling of shame and embarrassment, and reaction to stigma(turning to concealment, cautious disclosure, and marginalization. Shame and stigma were the major experiences of Iranian mothers of deaf children that shadowed their lives. These experiences lead mainly to ineffective coping mechanisms such as avoidance of using hearing aids and concealment of the child’s hearing loss. In addition, to cope with perceived stigma, mothers isolate and marginalize themselves and pursue secrecy strategies. Therefore, our findings are important for health professionals who are working with families having children with hearing loss. They need to aware of the problems faced by the families and should advocate necessary support.

  4. Wee Care: Reaching Teenage Mothers and Changing Their Lives.

    Science.gov (United States)

    Rosenwald, Priscilla R.; Porter, Gwen

    1989-01-01

    Describes an intervention program for pregnant teenagers and teen mothers. Topics include program development and implementation, staffing, and content. Also discussed are adjustments that a mother must make in order to break the chain of poverty and welfare. (RJC)

  5. Resettlement and reintegration: single mothers' reflections after homelessness

    OpenAIRE

    Tischler, Victoria

    2008-01-01

    Previous research has identified that most families who become homeless are women with dependent children. Homeless families are reported to have a variety of complex needs however little is known about the experiences of families once they are re-housed. The aim of this study was to explore psychosocial issues related to the resettlement experiences of single mothers following a period of homelessness. Qualitative semi-structured interviews were used to gather data from twenty one women livi...

  6. One uterus bridging three generations: first live birth after mother-to-daughter uterus transplantation.

    Science.gov (United States)

    Brännström, Mats; Bokström, Hans; Dahm-Kähler, Pernilla; Diaz-Garcia, Cesar; Ekberg, Jana; Enskog, Anders; Hagberg, Henrik; Johannesson, Liza; Kvarnström, Niclas; Mölne, Johan; Olausson, Michael; Olofsson, Jan I; Rodriguez-Wallberg, Kenny

    2016-08-01

    To determine whether a uterus from the mother of a woman with absolute uterine factor infertility can be transplanted to daughter and carry a pregnancy with delivery of a healthy child. Part of an observational study. University teaching hospital. Twenty eight-year-old woman with uterine agenesis, her male partner, and her 50-year-old mother. In vitro fertilization with embryo cryopreservation before live donor uterus transplantation (UTx). Induction immunosuppression. Embryo transfer 12 months after UTx, pregnancy controls, delivery, and hysterectomy. Results of IVF-ET, parameters of pregnancy/birth, and surgical data of transplantation/cesarean section/hysterectomy. Two IVF cycles before UTx resulted in 10 cryopreserved embryos. Donor surgery included hysterectomy with vascular pedicles of uterine vessels and proximal vessels up to and including parts of internal iliacs. Recipient surgery was by bilateral vascular connections to external iliacs, vaginal-vaginal anastomosis, and uterine fixation. Pregnancy occurred at the first single ET, and the pregnancy proceeded uneventfully until gestational week 34, when the patient developed cholestasis with intense pruritus. Cesarean section was performed at 34+6, with delivery of a healthy boy (weight 2,335 g). Hysterectomy was performed 3.5 months after delivery. The weight of the healthy child at 12 months was 9.3 kg. Grandmother (uterus donor) and mother are in good health 3 years after UTx. This is the first report of a live birth after mother-to-daughter UTx, and it also represents the second birth ever after human UTx. NCT01844362. Copyright © 2016. Published by Elsevier Inc.

  7. Octopus Moms: The Lived Experiences of College Students Who Are Mothers

    Science.gov (United States)

    Zambito, Jill

    2017-01-01

    The purpose of this phenomenological study was to explore the lived experiences of college students who are mothers at a large, public Midwestern research university. Using a feminist theoretical perspective, the focus of the research was to better understand how students who are mothers experience college. I sought to understand how the gendered…

  8. The Lived Experiences of Mothers whose Children were Sexually ...

    African Journals Online (AJOL)

    Child sexual abuse is a global phenomenon that affects many families and appears to be increasing dramatically in South Africa. The literature on child sexual abuse focuses mainly on the victims and perpetrators while largely ignoring the experiences of non-offending mothers. The objective of this study was to explore the ...

  9. Singled Out for Success: A Narrative Inquiry of Single Mothers in the Community College

    Science.gov (United States)

    Bober, Delia A.

    2017-01-01

    The purpose of this qualitative study was to explore and understand single-mother community college students' perceptions of their ability to succeed. The theoretical framework that guided this research was Bandura's (1977) social cognitive theory concept of self-efficacy, defined as a person's belief in his or her ability to succeed. Given…

  10. Primero Madres: Love and Mothering in the Educational Lives of Latina/os

    Science.gov (United States)

    Velazquez, Mirelsie

    2017-01-01

    This article examines the historical and contemporary role of Latina madres in the educational lives of their children and communities. Latinas, in their work as mother-activists, have played critical roles in the schooling lives of their children, seeking educational equality for their communities in general, amidst the growing racial politics…

  11. Mother-child discrepancy in perceived parental control and adolescent filial piety in poor single-mother families.

    Science.gov (United States)

    Leung, Janet T Y; Shek, Daniel T L; Lin, Li

    2017-10-01

    Based on a sample of 432 poor Chinese single-mother families (mean age of adolescents = 13.7 years; 51.2% girls; mean age of mothers = 43.5 years) in Hong Kong, the interaction effect of mother-reported and adolescent-reported maternal control on filial piety of Chinese adolescents was examined. Results of polynomial multiple regression analyses showed that the interaction between mother-reported and adolescent-reported maternal control predicted perceived filial piety in adolescents. At high levels of mother-reported maternal control, high adolescent-perceived parental control was associated with higher filial piety. At low levels of mother-reported maternal control, filial piety increased initially and then decreased when adolescents reported higher levels of maternal control. Using multiple group analyses, these associations were found to be stable across gender and age. The present findings provide insights on how congruencies and discrepancies between mother-reported and adolescent-reported maternal control predict filial piety of Chinese adolescents growing up in poor single-mother families. Copyright © 2017 The Foundation for Professionals in Services for Adolescents. Published by Elsevier Ltd. All rights reserved.

  12. 'Normal' and 'failing' mothers: Women's constructions of maternal subjectivity while living with multiple sclerosis.

    Science.gov (United States)

    Parton, Chloe; Katz, Terri; Ussher, Jane M

    2017-10-01

    Multiple sclerosis causes physical and cognitive impairment that can impact women's experiences of motherhood. This study examined how women construct their maternal subjectivities, or sense of self as a mother, drawing on a framework of biographical disruption. A total of 20 mothers with a multiple sclerosis diagnosis took part in semi-structured interviews. Transcripts were analysed using thematic decomposition to identify subject positions that women adopted in relation to cultural discourses of gender, motherhood and illness. Three main subject positions were identified: 'The Failing Mother', 'Fear of Judgement and Burdening Others' and 'The Normal Mother'. Women's sense of self as the 'Failing Mother' was attributed to the impact of multiple sclerosis, contributing to biographical disruption and reinforced through 'Fear of Judgement and Burdening Others' within social interactions. In accounts of the 'Normal Mother', maternal subjectivity was renegotiated by adopting strategies to manage the limitations of multiple sclerosis on mothering practice. This allowed women to self-position as 'good' mothers. Health professionals can assist women by acknowledging the embodied impact of multiple sclerosis on maternal subjectivities, coping strategies that women employ to address potential biographical disruption, and the cultural context of mothering, which contributes to women's experience of subjectivity and well-being when living with multiple sclerosis.

  13. The children of homosexual and heterosexual single mothers.

    Science.gov (United States)

    Javaid, G A

    1993-01-01

    Children reared in homes headed by homosexual and heterosexual mothers were compared with respect to the mothers' and children's attitudes towards marriage, procreation and homosexuality. The mothers did not prefer their children to be homosexual; they desired them to marry and procreate. This was expressed more unambiguously for their sons. The children mirrored these expectations, boys with greater frequency than the girls. Most of the children expressed reservations about having a homosexual mother.

  14. Perjuangan Tokoh Aoyagi Koharu sebagai Single Mother di Jepang dalam Drama Woman Karya Sutradara Nobuo Mizuta

    OpenAIRE

    ANGELINA, BUANA MARCHEL

    2014-01-01

    Angelina, Buana Marchel. 2014. “PERJUANGAN TOKOH AOYAGI KOHARU SEBAGAI SINGLE MOTHER DI JEPANG DALAM DRAMA WOMAN KARYA SUTRADARA NOBUO MIZUTA”, Program Studi Sastra Jepang, Jurusan Bahasa dan Sastra, Fakultas Ilmu Budaya, Universitas Brawijaya.Pembimbing : (1) Eka Marthanty Indah Lestari, M.Si (2) Iizuka Tasuku, MAKata Kunci: Drama, Perjuangan, Single Mother, Masyarakat Jepang.Skripsi ini membahas tentang perjuangan single mother dalam drama Woman. Perjuangan merupakan sebuah USAha atau ...

  15. Minimum Wages and the Economic Well-Being of Single Mothers

    Science.gov (United States)

    Sabia, Joseph J.

    2008-01-01

    Using pooled cross-sectional data from the 1992 to 2005 March Current Population Survey (CPS), this study examines the relationship between minimum wage increases and the economic well-being of single mothers. Estimation results show that minimum wage increases were ineffective at reducing poverty among single mothers. Most working single mothers…

  16. Work Socialization and Adolescents' Work-Related Values in Single-Mother African American Families

    Science.gov (United States)

    Toyokawa, Teru; McLoyd, Vonnie C.

    2013-01-01

    This study examined African American mothers' work socialization messages in relation to adolescents' work-related values. Moderation effects of mother-adolescent relation quality on the linkage between maternal socialization messages and adolescents' outcomes were also examined. Participants were 245 single African American mothers and their…

  17. Resilience as a moderator between syndemics and depression in mothers living with HIV.

    Science.gov (United States)

    Thurston, Idia B; Howell, Kathryn H; Kamody, Rebecca C; Maclin-Akinyemi, Courtney; Mandell, Jessica

    2018-03-02

    Physical and emotional adversities in mothers have rippling effects across the family system. While an association between individual maternal adversities and problematic mental health outcomes has been established, less is known about co-existing adversities in mothers. Consistent with the syndemic conceptual framework, we examined the co-occurrence of Substance Abuse, Violence, and AIDS/HIV (i.e., SAVA), which are three adversities that uniquely affect racial/ethnic minorities, individuals living in poverty, and people in urban communities. We assessed the relationship between SAVA adversities and depressive symptoms among mothers living with HIV, as well as the moderating effect of resilience on this relationship. Participants included 55 mothers (M age  = 41.24, SD = 9.01; 81% Black) living with HIV in the U.S. MidSouth. Mothers were recruited from community agencies serving individuals living with HIV and completed hour-long interviews about SAVA, depression, resilience, life stressors, and their child's mental health. Analyses were conducted in PROCESS for SPSS to test the relationship between SAVA and depression, as moderated by resilience. Analyses controlled for the influence of child maladaptive functioning (given known associations with maternal mental health) and maternal life stressors (given established associations with depressive symptoms). Findings indicated that experiencing more than one SAVA variable was associated with greater depressive symptoms (p resilience was associated with lower depressive symptoms (r = -.45; p resilience was low and weaker when resilience was high. Results not only highlight how co-occurring adversities exacerbate depressive symptoms, but also underscore the role of resilience as a key protective factor among mothers living with HIV. Resilience could therefore be a target of strengths-based treatment to reduce the negative effects of SAVA on depressive symptoms among mothers.

  18. Mothering in public: a meta-synthesis of homeless women with children living in shelters.

    Science.gov (United States)

    Meadows-Oliver, Mikki

    2003-01-01

    The purpose of this paper is to synthesize the current qualitative literature on homeless women with children living in shelters. Eighteen qualitative studies on homeless women with children living in shelters were included in the synthesis. The meta-synthesis was conducted using the meta-ethnographic approach of Noblit and Hare (1988). Six reciprocal translations (themes) of homeless mothers caring for their children in shelters emerged: On becoming homeless, protective mothering, loss, stressed and depressed, survival strategies, and strategies for resolution. The results may be used by healthcare workers as a framework for developing intervention strategies directed toward helping mothers find new solutions to dealing with shelter living and innovative ways to resolve their homelessness.

  19. The role of formal support in the lives of children of mothers with intellectual disability.

    Science.gov (United States)

    Collings, Susan; Grace, Rebekah; Llewellyn, Gwynnyth

    2017-05-01

    Mothers with intellectual disability face socioeconomic disadvantage and social isolation, which is associated with poorer child outcomes. Social services feature prominently in the lives of mothers with intellectual disability especially those without informal support; however, the role of formal support in the lives of their children has not been explored. Seven children aged 7-11 years took part in semi-structured interviews to explore their social experiences. Stories about interactions at home, school and with peers were analysed to illuminate influences, including social support, in their lives. Formal support can provide an enduring relationship for children from socially restricted homes when it focuses on the child's abilities, interests and needs as distinct from those of their mother. Child-focused support can be from a paid worker or volunteer. Family support services need to apply a child focus to improve the well-being of children in vulnerable families. © 2017 John Wiley & Sons Ltd.

  20. Living alone: exploring variations in single motherhood and child ...

    African Journals Online (AJOL)

    Two specifications were fitted to analyze the effect of single mother characteristics on child health using binomial logistic regression. The result of unadjusted and adjusted models indicates that never married, cohabiting, are important correlates of child health. When adjusted for covariates, the effect of single motherhood on ...

  1. How to stimulate single mothers on welfare to find a job : Evidence from a policy experiment

    NARCIS (Netherlands)

    Knoef, M.G.; van Ours, Jan

    2016-01-01

    We present the results from a policy experiment in which single mothers on welfare were stimulated to enter the labor market and increase their work experience. The aim of the policy was not per se for single mothers to leave welfare completely but to encourage them to find a job if only a part-time

  2. Single Black Working Mothers' Perceptions: The Journey to Achieve Leadership Positions

    Science.gov (United States)

    Raglin, Sherrell

    2017-01-01

    Single Black working mothers faced significant challenges in achieving high-level or senior-level leadership positions. The purpose of this qualitative narrative study was to collect, analyze and code the stories told by 10 participants to understand the perceptions and insights of the challenges and barriers single Black working mothers faced in…

  3. Poverty Trends for Families Headed by Working Single Mothers, 1993-1999.

    Science.gov (United States)

    Porter, Kathryn H.; Dupree, Allen

    This analysis examines poverty in families headed by working single mothers, addressing whether and to what degree their economic situations have improved. It investigates the effect of the 1996 Personal Responsibility and Work Opportunity Reconciliation Act, which moved single mothers from welfare to work. Poverty data from the annual Census…

  4. The Power of a Single Mother: The Influence of Black Women on Their Sons' Academic Performance

    Science.gov (United States)

    Robinson, Quintin L.; Werblow, Jacob

    2013-01-01

    This study examines the ways single Black mothers contribute to the educational success of their 11th-grade sons, despite the fact that their sons are enrolled in "failing schools." Data from five interviews and one focus group reveal common characteristics of how single-Black mothers help their sons beat the odds.

  5. Respite Care for Single Mothers of Children with Autism Spectrum Disorders

    Science.gov (United States)

    Dyches, Tina Taylor; Christensen, Ruthann; Harper, James M.; Mandleco, Barbara; Roper, Susanne Olsen

    2016-01-01

    Single mothers of children with autism spectrum disorders are rarely studied, yet they may experience unique stressors. Researchers asked 122 single mothers to complete questionnaires concerning respite care, daily hassles/uplifts, depression, and caregiver burden. More than half (59.8%) accessed respite care, which was provided for 1h per day,…

  6. Higher hair nicotine level in children compared to mother living with smoking father in Malaysia.

    Science.gov (United States)

    Mohamed, Nur Nadia; Loy, See Ling; Man, Che Nin; Al-Mamun, Abdullah; Jan Mohamed, Hamid Jan

    2016-11-01

    The objectives of this study are to determine parental and children's hair nicotine levels, their relationships as well as to investigate the association of smoking status of the fathers with mothers' and children's hair nicotine. A cross-sectional study design was conducted among 124 families who were participants of the Universiti Sains Malaysia Pregnancy Cohort Study. Both parents with their 2 years old children joined this study. A total of 92 hair samples of fathers, 124 hair samples of mothers and 111 hair samples of children were collected and analyzed by gas chromatography-mass spectrometry. Of total, 52.4 % of the fathers reported smoking. None of the mothers were smokers. Hair nicotine levels of fathers were found to be significantly correlated with mothers (r = 0.233, p = 0.026) and children (r = 0.508, p smoking fathers had significantly higher median hair nicotine level compared to the children of non-smoking fathers (6.08 vs 0.22 ng/mg, p = 0.046). However, this association was not seen in the mothers. Quantile regression showed significant association between fathers' and children's hair nicotine. There is a positive relationship between fathers' hair nicotine with mother's and children's hair nicotine. Living with smoking fathers can contribute to higher hair nicotine levels in children but not in mothers.

  7. The lived experience of mothers of multiple children with intellectual disabilities.

    Science.gov (United States)

    Kimura, Miyako; Yamazaki, Yoshihiko

    2013-10-01

    In this study, we explored the lived experience of Japanese mothers who have delivered multiple children with intellectual disabilities (ID), using interpretative phenomenological analysis. We identified three superordinate themes and seven subordinate themes from the narrative data collected from 10 participants. The superordinate themes were: abandoned hope for having an ordinary family, accumulating physical and mental fatigue, and searching for positive experiences in parenting multiple children with disabilities. How they perceived the birth of children with disabilities for the second time differed depending on the disability types; specifically, whether they detected the disabilities early or not. Encountering the disability in another child overwhelmed mothers, especially when the disabilities were diagnosed after several years with or without suspicion; they struggled to accept the fact. Despite mothers facing extreme difficulties in parenting multiple children with disabilities, they tried to alter the negative perceptions and find an optimistic way of living.

  8. Evidence of long-lived founder virus in mother-to-child HIV transmission.

    Directory of Open Access Journals (Sweden)

    Sivapragashini Danaviah

    Full Text Available Exposure of the infant's gut to cell-associated and cell-free HIV-1 trafficking in breast milk (BM remains a primary cause of mother-to-child transmission (MTCT. The mammary gland represents a unique environment for HIV-1 replication and host-virus interplay. We aimed to explore the origin of the virus transmitted during breastfeeding, and the link with quasi-species found in acellular and cellular fractions of breast-milk (BM and in maternal plasma. The C2-V5 region of the env gene was amplified, cloned and sequenced from the RNA and DNA of BM, the RNA from the mother's plasma (PLA and the DNA from infant's dried blood spot (DBS in 11 post-natal mother-infant pairs. Sequences were assembled in Geneious, aligned in ClustalX, manually edited in SeAL and phylogenetic reconstruction was undertaken in PhyML and MrBayes. We estimated the timing of transmission (ETT and reconstructed the time for the most recent common ancestor (TMRCA of the infant in BEAST. Transmission of single quasi-species was observed in 9 of 11 cases. Phylogenetic analysis illustrated a BM transmission event by cell-free virus in 4 cases, and by cell-associated virus in 2 cases but could not be identified in the remaining 5 cases. Molecular clock estimates, of the infant ETT and TMRCA, corresponded well with the timing of transmission estimated by sequential infant DNA PCR in 10 of 11 children. The TMRCA of BM variants were estimated to emerge during gestation in 8 cases. We hypothesize that in the remaining cases, the breast was seeded with a long-lived lineage latently infecting resting T-cells. Our analysis illustrated the role of DNA and RNA virus in MTCT. We postulate that DNA archived viruses stem from latently infected quiescent T-cells within breast tissue and MTCT can be expected to continue, albeit at low levels, should interventions not effectively target these cells.

  9. Cognitive Vulnerabilities to Depression for Adolescents in Single-Mother and Two-Parent Families

    Science.gov (United States)

    Daryanani, Issar; Hamilton, Jessica L.; McArthur, Brae Anne; Steinberg, Laurence; Abramson, Lyn. Y.; Alloy, Lauren B.

    2017-01-01

    Although research consistently suggests that adolescents in single-mother families are at increased risk for depression, the mechanisms that explain this relationship are unclear. In a community sample of adolescents (N = 368; ages 12–16; 50% female; 50% White) and their mothers (42% single), adolescents completed measures of depressive symptoms, rumination, and depressogenic inferential style at baseline and two yearly follow-ups. Mothers reported on stressful events that occurred in the child’s life from birth until baseline. Adolescents raised by single mothers, relative to partnered mothers, experienced more childhood stressors and higher rumination levels at one-year follow-up. Additionally, higher rumination mediated the relationship between single motherhood and greater youth depressive symptoms at the two-year follow-up. Clinical implications and developmental considerations are discussed. PMID:27858293

  10. Spiritual Journey in Mothers' Lived Experiences of Caring for Children With Autism Spectrum Disorders.

    Science.gov (United States)

    Heidary, Abbas; Hosseini Shahidi, Laleh; Mohammadpuor, Ali

    2015-03-30

    Helping mothers who have children with autism spectrum disorders requires understanding of their lived experiences. This study aims to uncover the spiritual journey as a main theme in Iranian mothers' experiences. This hermeneutic phenomenological study is a part of a larger study undertaken for partial fulfillment of the requirement for PhD dissertation in nursing. The main study was performed on 18 cases of Iranian mothers, with experience of caring for a child with an autism spectrum during 2011-2012. They were selected based on purposeful sampling method. Semi -structured interviews for data collection were used. Data analyses were done with the interpretative method. Spiritual journey is one of the main themes of the phenomenon under study in the original project. It consists of three sub -themes each of which supported by a number of common meaning. The sub-themes and their common meanings in parenthesis are (1) Descent: wondering between what is and what will be (having sorrowful tale, unanswered question, escaping from reality, losing hope) (2) Connecting to deity: reflection on the failure in her struggle (gratefulness, surrendering to god, having the divine test) (3) Ascent: helping her child is becoming all of the mother's life (to rescue, being hopeful, listening to her inner voice) This research concluded that caring for the autistic children led mothers' lives to raise spirituality and enabled them to help their children and themselves, to grow and be refined in this process.

  11. Contemporary Work and Family Issues Affecting Marriage and Cohabitation among Low-Income Single Mothers

    Science.gov (United States)

    Joshi, Pamela; Quane, James M.; Cherlin, Andrew J.

    2009-01-01

    In this paper, we advance and test an integrative model of the effects of employment status, nonstandard work schedules, male employment, and women's perceptions of economic instability on union formation among low-income single mothers. On the basis of the longitudinal data from 1,299 low-income mothers from the Three-City Welfare Study, results…

  12. Guia de Recursos para la Madre Sola. (Resource Guide for the Single Mother).

    Science.gov (United States)

    Martin-Major, Marilyn; Vrana, Margaret; Whitehouse, John; Bianchi, Mary Ann; Spath, Barry; Williams, Marian; Bones, David; Morar, Betty; Newbert, David

    Funded under the Women's Educational Equity Act, the Assertiveness Training Program for Single Mothers was offered to mothers with children enrolled in the Omaha Head Start and Parent-Child Center Programs. The 16-week long program, providing a total of 40 hours of training, covered a wide range of topics in addition to the initial workshops on…

  13. Doulas' Perceptions on Single Mothers' Risk and Protective Factors, and Aspirations Relative to Child-Birth

    Science.gov (United States)

    Arat, Gizem

    2013-01-01

    This study the author aims to explore the perceptions of doulas on single mothers' risk and protective factors, and aspirations relative to child-birth in the postpartum care. The current study was conducted by semi-structured questions, case file reviews, field notes, and twelve home visits via utilizing Grounded Theory. These mothers receive…

  14. Image-Based Single Cell Profiling: High-Throughput Processing of Mother Machine Experiments.

    Science.gov (United States)

    Sachs, Christian Carsten; Grünberger, Alexander; Helfrich, Stefan; Probst, Christopher; Wiechert, Wolfgang; Kohlheyer, Dietrich; Nöh, Katharina

    Microfluidic lab-on-chip technology combined with live-cell imaging has enabled the observation of single cells in their spatio-temporal context. The mother machine (MM) cultivation system is particularly attractive for the long-term investigation of rod-shaped bacteria since it facilitates continuous cultivation and observation of individual cells over many generations in a highly parallelized manner. To date, the lack of fully automated image analysis software limits the practical applicability of the MM as a phenotypic screening tool. We present an image analysis pipeline for the automated processing of MM time lapse image stacks. The pipeline supports all analysis steps, i.e., image registration, orientation correction, channel/cell detection, cell tracking, and result visualization. Tailored algorithms account for the specialized MM layout to enable a robust automated analysis. Image data generated in a two-day growth study (≈ 90 GB) is analyzed in ≈ 30 min with negligible differences in growth rate between automated and manual evaluation quality. The proposed methods are implemented in the software molyso (MOther machine AnaLYsis SOftware) that provides a new profiling tool to analyze unbiasedly hitherto inaccessible large-scale MM image stacks. Presented is the software molyso, a ready-to-use open source software (BSD-licensed) for the unsupervised analysis of MM time-lapse image stacks. molyso source code and user manual are available at https://github.com/modsim/molyso.

  15. [Growth, development, social integration and parenting practices on children living with their mothers in prison].

    Science.gov (United States)

    Lejarraga, Horacio; Berardi, Clemente; Ortale, Susana; Contreras, María Magdalena; Sanjurjo, Adriana; Lejarraga, Celina; Martínez Cáceres, María José; Rodríguez, Lilian

    2011-12-01

    Prisons are considered a very high risk environment, but there is very scarce scientific information on the growth and development of children who live there. With the purpose of evaluating growth and development of children living with their mothers in prison, a cross sectional survey was performed in 68 children aged 0-3 years in Unit 31, a prison for mothers with children located at 30 km from Buenos Aires. Weight, supine length, and developmental quotient (Cats Clams) was measured, PRUNAPE, and SQE-SE test were administered (screening tests for detecting developmental and emotional disorders, respectively). Mothers' child rearing practices were assessed with a previously standardized questionnaire. Age range of mothers was 18-46 years, median 23.5 yr. Duration of stay in prison of children was 0.05-35 months. Mean ± standard error of "Z" score for height was -0.30 ± 0.12, weight 0.40 ± 0.12; body mass index 0.91 ± 0.18; head circumference 0.19 ± 0.14; and developmental quotient 92.4 ± 1.7. There were only 7 children out of 67 who failed the PRUNAPE. Mean Cats Clams developmental score was 91.3. ASQ-SE test showed high prevalence (39%) of emotional and social integration problems. Mothers' knowledge about child rearing practices was poorer than that found in a normal urban local population. There were no trends for results to be related to the length of stay in prison. Children are shorter and with higher BMI than reference. Emotional disorders are frequent (39%). Mothers' knowledge about child care was poorer than that found in a near population. A program for longitudinal surveillance for all prisons in the country was proposed to the authorities.

  16. Child Care Choices and Children's Cognitive Achievement: The Case of Single Mothers

    OpenAIRE

    Raquel Bernal; Michael P. Keane

    2011-01-01

    We evaluate the effect of child care versus maternal time inputs on child cognitive development using single mothers from the NLSY79. To deal with nonrandom selection of children into child care, we exploit the exogenous variation in welfare policy rules facing single mothers. In particular, the 1996 welfare reform and earlier state-level policy changes generated substantial increases in their work/child care use. We construct a comprehensive set of welfare policy variables and use them as in...

  17. Child health care utilisation in families with young or single mothers in a Swedish county.

    Science.gov (United States)

    Wallby, Thomas; Modin, Bitte; Hjern, Anders

    2013-03-01

    Young age and lone parenthood are risk factors for impaired health among mothers and their children. Due to the higher risks of negative influences on physical and mental health, young and single mothers should be of special concern to the Child Health Services (CHS). In the present study, we investigated consumption patterns of child health care services among young and single mothers in Uppsala County, Sweden to study whether they are reached by the universal CHS program and if selective or indicative measures were administered in daily CHS practice. Register data on CHS contacts and socio-demographic indicators were collected for 10692 infants, born in 1998-2006. Results show small differences in contact pattern and immunization status, between children of young versus older, and single versus cohabiting mothers. However, both young (RR 0.64) and single (RR 0.80) mothers had significantly lower rates of participation in parental group. The CHS were consequently successful in implementing the universal preventive child health programme for all families, including families with young or single mothers. There was no indication, however, of an established selective preventive strategy aimed at these high risk families. Programs for strengthening the support provided to vulnerable families by the CHS are needed. © The Author(s) 2012.

  18. Work life and mental well-being: single and coupled employed mothers in Southern Europe and Scandinavia.

    Science.gov (United States)

    Bull, Torill

    2009-09-01

    Many European mothers, single and coupled, combine work outside the home and family life. The effects of this on their mental well-being may vary depending on the level of support available from the State's welfare system, since welfare may buffer working mothers from some of the stress that can arise from trying to manage significant responsibilities on the job and at home. Welfare may be especially important for single working mothers, for whom the burden of multiple roles may be even heavier. The present study assessed levels and predictors of well-being of single and coupled employed mothers in Greece, Portugal and Spain, where welfare support is relatively limited. Results were compared to a parallel study with data from Denmark, Norway and Sweden, where welfare support is relatively comprehensive. Coupled mothers in Scandinavia had significantly lower financial hardship, longer education, higher life satisfaction, more enriching jobs, practical support, financial support and social participation than coupled mothers in the Southern European sample. On the other hand, the Scandinavian coupled mothers had higher levels of work family conflict than coupled mothers in Southern Europe. Single mothers in Scandinavia, compared to single mothers in Southern Europe, had significantly longer education, higher life satisfaction and positive affect, more enriching jobs, confidant support, practical support, financial support and social participation. Level of job stress was the same for all mother groups. All groups differed significantly from each other in level of financial hardship, with Scandinavian coupled mothers being best off, followed by Scandinavian single mothers, Southern European coupled mothers, and Southern European single mothers. The regional differences suggest that single motherhood per se need not be a risk factor for poorer well-being, and that welfare policies may have a protective effect for the mental well-being of single mothers.

  19. Embodied drawings as expressions of distress among impoverished single Bedouin mothers.

    Science.gov (United States)

    Huss, Ephrat; Cwikel, Julie

    2008-06-01

    This paper demonstrates how marginalized, Bedouin, single mothers define pain through different depictions of their bodies and their embodied experience. Using visual data generated through an empowerment group with single Bedouin women living in the Negev, illustrative pictures were selected. The potential of drawing as an indirect, but deeply communicative symbolic vehicle with which to express the women's pain and struggle as marginalized and impoverished women is demonstrated through themes that emerged from a content analysis of the women's art and their verbal comments about what they had drawn. A central theme identified pain due to painful life circumstances, rather than due to inherent sickness or weakness. Other themes identified included the body as a site for cultural transition, power negations with men, intellectual development, and the struggles of motherhood. This shows how the visual depiction of pain on the page offers a socially critical, yet potentially mental health promoting medium that locates women's distress, not as the result of personal and physical weakness, but as the result of social oppression. The implications for the use of art with socially marginalized women are discussed.

  20. Epidemiological profile of Trypanosoma cruzi-infected mothers and live birth conditions in the state of Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    Fabiane Scalabrini Pinto

    2013-04-01

    Full Text Available INTRODUCTION: The aim of this study was to assess the epidemiological characteristics of Trypanosoma cruzi-infected mothers and the live birth conditions of neonates. METHODS: A serological survey with IgG-specific tests was conducted using dried blood samples from newborn infants in the State of Minas Gerais. T. cruzi infection was confirmed in mothers through positive serology in two different tests, and infected mothers were required to have their infants serologically tested after the age of 6 months. The birth conditions of the neonates were obtained from the System of Information on Live Births database. RESULTS: The study included 407 children born to T. cruzi-infected mothers and 407 children born to uninfected mothers. The average age of seropositive mothers was 32 years (CI95% 31.3-32.6, which was greater than the average age of seronegative mothers - 25 years (CI95% 24.8-25.2. The mothers' level of education was higher among uninfected mothers (41% had 8 or more years of education, versus 22% between the infected mothers. Vaginal delivery was more frequent among infected mothers. There was no evidence of inter-group differences with respect to the child's sex, gestational age, birth weight or Appearance, pulse, grimace, activity and respiration (APGAR scores at 1 and 5 minutes. Conclusions: The level of education and the greater number of previous pregnancies and cases of vaginal delivery reflect the lower socioeconomical conditions of the infected mothers. In the absence of vertical transmission, neonates had similar health status irrespective of the infection status of their mothers.

  1. Physical Activity Experiences and Beliefs among Single Mothers: A Qualitative Study

    Science.gov (United States)

    Dlugonski, Deirdre; Motl, Robert W.

    2016-01-01

    Purpose: Single motherhood has been associated with negative health consequences such as depression and cardiovascular disease. Physical activity might reduce these consequences, but little is known about physical activity experiences and beliefs that might inform interventions and programs for single mothers. The present study used…

  2. Does Single Motherhood Hurt Infant Health among Young Mothers?

    OpenAIRE

    Kim, Albert Young-Il; Lee, Jungmin

    2017-01-01

    Does single motherhood adversely affect infant health? This question is not easy to answer because of the endogeneity of coresidence during pregnancy. In this paper, we exploit quasi-natural variation in single motherhood from the moment of conception to that of birth arising from marriageable age restrictions and the reform of the laws in Korea. The Korean birth certificate dataset is unique in that it allows us to distinguish coresidence and legal marital status and further to identify the ...

  3. The lived experience of Taiwanese mothers of a child diagnosed with adrenoleukodystrophy.

    Science.gov (United States)

    Lee, Tsorng-Yeh; Li, Chia-Chi; Liaw, Jen-Jiuan

    2014-02-01

    This research was conducted to describe Taiwanese mothers' lived experience of caring for their child diagnosed with adrenoleukodystrophy. Analysis of eight interviews by Colaizzi's method revealed six themes: (1) difficulty confirming the diagnosis; (2) powerlessness toward unsatisfactory treatment; (3) struggles with decisions around carrier testing; (4) guilt about being a carrier; (5) support from family, other parents, and religion; and (6) lack of integrated resources and support. The results suggest the need to raise public and physician awareness about adrenoleukodystrophy via the media and medical education. A central organization to provide parents with comprehensive information is needed.

  4. Violence in the Lives of Incarcerated Aboriginal Mothers in Western Australia

    Directory of Open Access Journals (Sweden)

    Mandy Wilson

    2017-01-01

    Full Text Available Drawing on in-depth interviews with incarcerated Aboriginal and Torres Strait Islander mothers in Western Australia, we report on the women’s use of violence in their relationships with others. Results reinforce that Aboriginal women are overwhelmingly victims of violence; however, many women report also using violence, primarily as a strategy to deal with their own high levels of victimization. The “normalization” of violence in their lives and communities places them at high risk of arrest and incarceration. This is compounded by a widespread distrust of the criminal justice system and associated agencies, and a lack of options for community support.

  5. The quality of life of single mothers making the transition from welfare to work.

    Science.gov (United States)

    Cook, Kay; Davis, Elise; Smyth, Paul; McKenzie, Hayley

    2009-09-01

    This study examined the quality of life of single mothers making the mandatory transition from welfare to work. The Australian government purported that the benefits of making this transition would include higher incomes, better social participation, and improved wellbeing. It is currently unknown, however, how single mothers currently engaged in welfare to work programs evaluate their quality of life. Quality of life scores for 334 single mothers engaged in welfare to work in Australia were compared with normative data. Participants reported significantly lower quality of life scores than the general population for all quality of life domains, highlighting the need to carefully examine welfare to work policies to ensure they promote participants' quality of life.

  6. Psychosocial differences related to parenting infants among single and married mothers.

    Science.gov (United States)

    Copeland, Debra Beach; Harbaugh, Bonnie Lee

    2010-01-01

    This study compared the psychosocial factors, self-esteem, sense of mastery, life stress, and social support among first-time married and single mothers in early parenthood. A cross-sectional comparative design was used to study secondary data. A convenience sample of 80 first-time mothers completed self-report instruments on self-esteem, sense of mastery, social, and life stress at 6-8 weeks after birth. The data were analyzed using descriptive statistics, chi-square tests and ANOVA. A statistically significant difference was found between married and single mothers on self esteem (F = 9.314, df = 1, p = .003) and sense of mastery (F = 4.919, df = 1, p = .030). There were no statistical differences found between married and single mothers on social support and life stress. Findings support Belsky's (1984) theoretical assertion that personal psychological resources are most indicative of successful parenting. Further, interventions directed toward enhancing single mothers' sense of mastery and self-esteem may be needed to facilitate maternal role transition.

  7. THE DEAD-LIVING-MOTHER: MARIE BONAPARTE'S INTERPRETATION OF EDGAR ALLAN POE'S SHORT STORIES.

    Science.gov (United States)

    Obaid, Francisco Pizarro

    2016-06-01

    Princess Marie Bonaparte is an important figure in the history of psychoanalysis, remembered for her crucial role in arranging Freud's escape to safety in London from Nazi Vienna, in 1938. This paper connects us to Bonaparte's work on Poe's short stories. Founded on concepts of Freudian theory and an exhaustive review of the biographical facts, Marie Bonaparte concluded that the works of Edgar Allan Poe drew their most powerful inspirational force from the psychological consequences of the early death of the poet's mother. In Bonaparte's approach, which was powerfully influenced by her recognition of the impact of the death of her own mother when she was born-an understanding she gained in her analysis with Freud-the thesis of the dead-living-mother achieved the status of a paradigmatic key to analyze and understand Poe's literary legacy. This paper explores the background and support of this hypothesis and reviews Bonaparte's interpretation of Poe's most notable short stories, in which extraordinary female figures feature in the narrative.

  8. Bacterial Composition and Diversity in Breast Milk Samples from Mothers Living in Taiwan and Mainland China

    Directory of Open Access Journals (Sweden)

    Shiao-Wen Li

    2017-05-01

    Full Text Available Human breast milk is widely recognized as the best source of nutrients for healthy growth and development of infants; it contains a diverse microbiota. Here, we characterized the diversity of the microbiota in the breast milk of East Asian women and assessed whether delivery mode influenced the microbiota in the milk of healthy breast-feeding mothers. We profiled the microbiota in breast milk samples collected from 133 healthy mothers in Taiwan and in six regions of mainland China (Central, East, North, Northeast, South, and Southwest China by using 16S rRNA pyrosequencing. Lactation stage (months postpartum when the milk sample was collected and maternal body mass index did not influence the breast milk microbiota. Bacterial composition at the family level differed significantly among samples from the seven geographical regions. The five most predominant bacterial families were Streptococcaceae (mean relative abundance: 24.4%, Pseudomonadaceae (14.0%, Staphylococcaceae (12.2%, Lactobacillaceae (6.2%, and Oxalobacteraceae (4.8%. The microbial profiles were classified into three clusters, driven by Staphylococcaceae (abundance in Cluster 1: 42.1%, Streptococcaceae (Cluster 2: 48.5%, or Pseudomonadaceae (Cluster 3: 26.5%. Microbial network analysis at the genus level revealed that the abundances of the Gram-positive Staphylococcus, Streptococcus, and Rothia were negatively correlated with those of the Gram-negative Acinetobacter, Bacteroides, Halomonas, Herbaspirillum, and Pseudomonas. Milk from mothers who had undergone Caesarian section (C-section group had a significantly higher abundance of Lactobacillus (P < 0.05 and a higher number of unique unclassified operational taxonomic units (OTUs (P < 0.001 than that from mothers who had undergone vaginal delivery (vaginal group. These findings revealed that (i geographic differences in the microbial profiles were found in breast milk from mothers living in Taiwan and mainland China, (ii the

  9. Lived experiences of Jordanian mothers caring for a child with disability.

    Science.gov (United States)

    Nazzal, Mohammad S; Al-Rawajfah, Omar M

    2017-07-18

    Caring for a child with a disability in the family is associated with a major increase in care-giving demands and burden. This qualitative study explores the perspectives, challenges and adaptations of Jordanian mothers living with a child with disability. Phenomenological qualitative tradition was utilized. Seven Jordanian mothers of children with disability were purposefully selected as having rich experiences for caring for a child with a disability. In-depth interviews were conducted using a semi-structured guide, audio-taped and transcribed verbatim. Interpretive phenomenological analysis was used to extract main themes and subthemes. Mothers' experiences were reflected into four main themes: (1) increased perceived stigma, (2) fear for the future, (3) increased perceived care-giving burden and (4) adaptations to the child's disability. This study highlights key gaps in the provision of family-centered services for this population as well as multiple sociocultural issues impacting participation and quality of life. Healthcare professionals must deal with the child's disability from a family-centered and cultural perspective. Implications for rehabilitation When therapists meet the whole family's needs through a family-centered approach, the child with disability is less likely to be ignored, maltreated or abused. Family-centered practice adopts a sociocultural model which looks at the child from a wider point of view rather than just the disability itself. It requires therapists to review the context in which the child lives and address the specific needs of parents, siblings and other involved family members. Culture plays a huge role in shaping the family's perspective on disability and has a huge impact and implications for service delivery and development, quality of life and participation for children with disability and their families. Rehabilitation professionals must accommodate their time schedules to provide families with the communication, education

  10. Homelessness Pathways for Australian Single Mothers and Their Children: An Exploratory Study

    Directory of Open Access Journals (Sweden)

    Wayne Warburton

    2018-03-01

    Full Text Available There is increasing concern about family homelessness. Homeless mothers and their children are one of society’s most disadvantaged and at-risk populations. However, very little Australian research exploring mothers’ views on their homelessness experiences exists. Using semi-structured interviews with 14 mothers and four agency staff, this study explored homeless Australian mothers’ pathways into and out of homelessness, their specific needs and the services and supports that were (or would have been most helpful. In this sample of single mothers and their children, early experiences of homelessness and domestic violence contributed most commonly to homelessness episodes. Almost immediate engagement with welfare agencies seemed to be protective against re-experiencing homelessness, however Australian restrictions on length of program involvement and limited housing options for mothers exiting homelessness programs, may place such mothers and their children at high risk of re-entering homelessness. Younger mothers had greater needs and benefited most from personalised one-on-one support that addressed key parenting and life skills. The implications of these findings are considered in relation to service delivery to this vulnerable group and avenues for future research are noted.

  11. How to Stimulate Single Mothers on Welfare to Find a Job : Evidence from a Natural Experiment

    NARCIS (Netherlands)

    Knoef, M.G.; van Ours, J.C.

    2014-01-01

    We present the results from a natural experiment in which single mothers on welfare were stimulated to find a job. Two policy instruments were introduced: an earnings disregard and job creation. The experiment was performed at the level of municipalities in The Netherlands, a country with relatively

  12. Financial Well-Being of Single, Working-Age Mothers of Children with Developmental Disabilities

    Science.gov (United States)

    Parish, Susan L.; Rose, Roderick A.; Swaine, Jamie G.; Dababnah, Sarah; Mayra, Ellen Tracy

    2012-01-01

    Understanding the financial well-being of single mothers who care for children with developmental disabilities is important to ensure that public policies can be effectively targeted to support these vulnerable families. The authors analyze data from the Survey of Income and Program Participation to describe income poverty, asset poverty, income,…

  13. Low-Income, Single-Parent Francophone Mothers and the Educational Achievement of Their Children

    Science.gov (United States)

    Caissie, Julie; Gaudet, Jeanne d'Arc; Godin, Jeanne

    2017-01-01

    This phenomenological study focused on single-parent, low-income francophone mothers' relationships with the educational and cultural achievement of their children attending French-speaking schools in Anglo-dominant settings in New Brunswick (Canada). We conducted individual (N = 8) semi-structured interviews to solicit information about the…

  14. The Interplay of Autonomy and Relatedness in Hong Kong Chinese Single Mothers

    Science.gov (United States)

    Choy, Winnie C. W.; Moneta, Giovanni B.

    2002-01-01

    The mediating effects of autonomy and relatedness on the relationship between self-esteem and life satisfaction were investigated among 49 single mothers. Among all tested variables in the dimension of relatedness, only network orientation (propensity to utilize one's available social support) was a mediator of the positive relationship between…

  15. Types of work-related behavior and experiences and stress coping strategies among single mothers and mothers in relationships differentiating role of work satisfaction.

    Science.gov (United States)

    Napora, Elżbieta; Andruszkiewicz, Anna; Basińska, Małgorzata Anna

    2018-01-01

    The purpose of the study has been to describe functioning of single and mothers in relationships (married or in informal relationships) at work and verify if the declared degree of work satisfaction differentiates types of behavior at work and stress coping strategies in both groups of mothers. The study was conducted on equal samples of single mothers (N = 186) and mothers from 2-parent families (N = 186) using Latack Coping Scale that measures work-related stress coping strategies, the AVEM (Arbeitsbezogenes Verhaltens- und Erlebensmuster - Work-Related Behavior and Experience Pattern) questionnaire, and a survey. It showed similarity between the studied groups in terms of the measured variables. There were considerable differences between single and married mothers in terms of support seeking strategies. The interaction of work satisfaction and the type of motherhood significantly differentiates (p = 0.03) the avoidance strategy of resignation. That strategy of resignation was more frequently used by single mothers with lower work satisfaction, who were distinctly different from those whose work satisfaction was higher, and from the mothers in relationships (married or in informal relationships) (regardless of the level of their work satisfaction). Int J Occup Med Environ Health 2018;31(1):55-69. This work is available in Open Access model and licensed under a CC BY-NC 3.0 PL license.

  16. An approach to emotion recognition in single-channel EEG signals: a mother child interaction

    Science.gov (United States)

    Gómez, A.; Quintero, L.; López, N.; Castro, J.

    2016-04-01

    In this work, we perform a first approach to emotion recognition from EEG single channel signals extracted in four (4) mother-child dyads experiment in developmental psychology. Single channel EEG signals are analyzed and processed using several window sizes by performing a statistical analysis over features in the time and frequency domains. Finally, a neural network obtained an average accuracy rate of 99% of classification in two emotional states such as happiness and sadness.

  17. Some problems of parametric neutron activation analysis based on the use of radioactive daughters of longer-lived mothers with low mother/daughter half-life ratios

    International Nuclear Information System (INIS)

    Cohen, I.M.

    2012-01-01

    The theoretical and practical aspects of the use of radioactive daughters originated from the decay of longer-lived radioactive mothers in parametric activation analysis, when the ratio: mother half-life to daughter half-life is less than 10, are discussed. The mother-daughter relationships: 47 Ca/ 47 Sc; 95 Zr/ 95 Nb; 140 Ba/ 140 La; 99 Mo/ 99m Tc and 115 Cd/ 115m In are selected as models for the study. The cases when the radionuclide of interest is formed through both direct and indirect routes are also analyzed. As illustrative example, the direct reaction and the reaction chain: 47 Ti(n,p) 47 Sc/ 46 Ca(n,γ) 47 Ca(β - ) 47 Sc are evaluated with respect to the determination of the elements involved and their reciprocal interferences. (author)

  18. Single-molecule tracking in living cells using single quantum dot applications.

    Science.gov (United States)

    Baba, Koichi; Nishida, Kohji

    2012-01-01

    Revealing the behavior of single molecules in living cells is very useful for understanding cellular events. Quantum dot probes are particularly promising tools for revealing how biological events occur at the single molecule level both in vitro and in vivo. In this review, we will introduce how single quantum dot applications are used for single molecule tracking. We will discuss how single quantum dot tracking has been used in several examples of complex biological processes, including membrane dynamics, neuronal function, selective transport mechanisms of the nuclear pore complex, and in vivo real-time observation. We also briefly discuss the prospects for single molecule tracking using advanced probes.

  19. "How Can You Live without Your Kids?": Distancing from and Embracing the Stigma of "Incarcerated Mother."

    Science.gov (United States)

    Aiello, Brittnie; McQueeny, Krista

    2016-01-01

    This article examines how incarcerated mothers constructed moral identities in the face of stigma. Analyzing data from participant observation and 83 in-depth interviews with incarcerated mothers, we show that mothers claimed moral identities by distancing from the stigma of incarceration and/or embracing the identity of incarcerated mothers.…

  20. Women and Work-Life Balance: A Narrative Inquiry of Working Single Mothers Balancing Family and Work

    Science.gov (United States)

    Stephens, Casheena A.

    2017-01-01

    The purpose of this study was to explore working single mothers' work-life balance in order to better understand how employers can assist them. Role theory, role conflict theory, and spillover theory were utilized to examine how working single mothers experience work-life balance and how they perceive it. In this study, the researcher sought to…

  1. Single Particle Tracking: Analysis Techniques for Live Cell Nanoscopy

    Science.gov (United States)

    Relich, Peter Kristopher, II

    Single molecule experiments are a set of experiments designed specifically to study the properties of individual molecules. It has only been in the last three decades where single molecule experiments have been applied to the life sciences; where they have been successfully implemented in systems biology for probing the behaviors of sub-cellular mechanisms. The advent and growth of super-resolution techniques in single molecule experiments has made the fundamental behaviors of light and the associated nano-probes a necessary concern amongst life scientists wishing to advance the state of human knowledge in biology. This dissertation disseminates some of the practices learned in experimental live cell microscopy. The topic of single particle tracking is addressed here in a format that is designed for the physicist who embarks upon single molecule studies. Specifically, the focus is on the necessary procedures to generate single particle tracking analysis techniques that can be implemented to answer biological questions. These analysis techniques range from designing and testing a particle tracking algorithm to inferring model parameters once an image has been processed. The intellectual contributions of the author include the techniques in diffusion estimation, localization filtering, and trajectory associations for tracking which will all be discussed in detail in later chapters. The author of this thesis has also contributed to the software development of automated gain calibration, live cell particle simulations, and various single particle tracking packages. Future work includes further evaluation of this laboratory's single particle tracking software, entropy based approaches towards hypothesis validations, and the uncertainty quantification of gain calibration.

  2. Single particle labeling of RNA virus in live cells.

    Science.gov (United States)

    Liu, Xiaohui; Ouyang, Ting; Ouyang, Hongsheng; Ren, Linzhu

    2017-06-02

    Real-time and visual tracking of viral infection is crucial for elucidating the infectious and pathogenesis mechanisms. To track the virus successfully, an efficient labeling method is necessary. In this review, we first discuss the practical labeling techniques for virus tracking in live cells. We then describe the current knowledge of interactions between RNA viruses (especially influenza viruses, immunodeficiency viruses, and Flaviviruses) and host cellular structures, obtained using single particle labeling techniques combined with real-time fluorescence microscopy. Single particle labeling provides an easy system for understanding the RNA virus life cycle. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Single-photon ultrashort-lived radionuclides: symposium proceedings

    Energy Technology Data Exchange (ETDEWEB)

    Paras, P.; Thiessen, J.W. (eds.)

    1985-01-01

    The purpose was to define the current role and state-of-the-art regarding the development, clinical applications, and usefulness of generator-produced single-photon ultrashort-lived radionuclides (SPUSLR's) and to predict their future impact on medicine. Special emphasis was placed on the generator production of iridium-191, gold-195, and krypton-81. This report contains expanded summaries of the included papers. (ACR)

  4. Health care of children living with their mother in prison compared with the general population.

    Science.gov (United States)

    Ferrara, Pietro; Gatto, Antonio; Nicoletti, Alessandro; Emmanuele, Valentina; Fasano, Alfonso; Currò, Vincenzo

    2009-05-01

    Italian law allows female prisoners in specific "nest areas" to keep their babies with them until the child reaches the age of 3 years. We describe health care of children living in the nest areas and we compare this condition with the general population. Our study was conducted in the female detention centre of Rome Rebibbia, Italy. Clinical diaries of all babies have been screened. The pediatrics ambulatory of A. Gemelli Hospital of Rome provided all data for comparison. We describe 391 children (150 in the prison group, G1; 150 children with Italian parents, G2, and 91 children with foreign parents, G3) with similar characteristics. There were statistical differences between-groups in respect of the auxological data, gestational age (lower in G1 vs G2, p G2, p G3, p prison. According to our observations children received good sanitary assistance inside the prison and the adjustment of their immunisation status when they stayed in prison for a long enough period. The significant information shown by our data suggest that children living in prison with their mothers represent a vulnerable group with particular healthcare needs.

  5. Identifying Unmet Mental Health Needs in Children of Formerly Homeless Mothers Living in a Supportive Housing Community Sector of Care

    Science.gov (United States)

    Lee, Susanne S.; August, Gerald J.; Gewirtz, Abigail H.; Klimes-Dougan, Bonnie; Bloomquist, Michael L.; Realmuto, George M.

    2010-01-01

    This study reports psychosocial characteristics of a sample of 111 children (K to 2nd grade) and their mothers who were living in urban supportive housings. The aim of this study was to document the various types and degree of risk endemic to this population. First, we describe the psychosocial characteristics of this homeless sample. Second, we…

  6. Having another child without intellectual disabilities: Comparing mothers of a single child with disability and mothers of multiple children with and without disability.

    Science.gov (United States)

    Kimura, Miyako; Yamazaki, Yoshihiko

    2017-01-01

    To investigate how having a child without a disability is related to maternal mental health and its predictors, we compared mothers of a single child with intellectual disabilities (single-child group, n = 117) and mothers of children with and without intellectual disabilities (multiple-children group, n = 457), in Japan. Compared to the multiple-children group, the single-child group had lower hope and social capital and poorer mental health; furthermore, 42.5% of them had decided not to have another child, despite desiring one. Hope was the strongest predictor of mental health in both groups but was more positively related to mental health in the multiple-children group. The fear of the possibility of the next child having a disability and receiving inadequate support were the obstacles for mothers who had decided not to have another child. Thus, greater support and more opportunities for obtaining adequate information about the implications of having another child may be needed.

  7. The birds, the bees, and the Bible: single African American mothers' perceptions of a faith-based sexuality educaton program.

    Science.gov (United States)

    Cornelius, Judith Bacchus

    2009-01-01

    This exploratory study examined single mothers' ideas on the development of a faith-based sexuality program. Twenty African American single mothers with adolescent children (11 to 13 years of age) who were of the same faith and members of one church, participated in two focus groups about how a faith-based sexuality program could be designed and implemented. The findings call attention to the need for research on the design of faith-based sexuality education programs for ethnic minority families headed by single mothers.

  8. Beating the Odds: How Single Black Mothers Influence the Educational Success of Their Sons Enrolled in Failing Schools

    Science.gov (United States)

    Robinson, Quintin L.; Werblow, Jacob

    2012-01-01

    The academy has given little attention to academically successful Black males and the factors that may lead to their successes. This multiple case study design, however, examined the ways in which single-Black mothers influence the educational success of their sons by focusing on the mothers of academically successful 11th grade Black males. Data…

  9. Immunogenicity of single dose live attenuated hepatitis a vaccine.

    Science.gov (United States)

    Bhave, Sheila; Bavdekar, Ashish; Sapru, Amita; Bawangade, Seema; Pandit, Anand

    2011-02-01

    A long-term immunogenicity study of a single dose live attenuated H2 strain hepatitis A vaccine is being conducted in healthy Indian children at KEM Hospital, Pune. 131 of the original 143 children vaccinated in 2004, were evaluated for anti-HAV antibodies 30 months post vaccination (2007). Seroprotective antibody levels >20 mIU/mL were demonstrated in 87.8 % subjects with an overall GMT of 92.02 mIU/mL. No hepatitis like illness was recorded in any of the subjects since vaccination.

  10. Paying to waste lives: the affordability of reducing mother-to-child transmission of HIV in South Africa.

    Science.gov (United States)

    Skordis, Jolene; Nattrass, Nicoli

    2002-05-01

    It is estimated that each HIV-positive child in South Africa costs the government more in terms of health and welfare expenses than it does to reduce mother-to-child transmission (MTCT) of HIV through the use of antiretroviral regimens (where the mother continues to breast-feed). Programmes to reduce MTCT of HIV/AIDS are, thus, clearly affordable. Using Nevirapine (according to the HIVNET 012 Protocol) saves more lives and [corrected] is more cost-effective than using Zidovudine (CDC 2 weeks regime).

  11. Single mothers and their sense of purpose and meaning in life [Poczucie sensu życia matek samotnie wychowujących dzieci

    Directory of Open Access Journals (Sweden)

    Lilianna KLIMEK

    2017-11-01

    Full Text Available The article presents the results of a research on single mothers and their sense of purpose and meaning in life. Questions about the meaning of one’s own existence recur, with varying intensity, in the consciousness of every human being. Moreover, people always fulfil their own, personal meaning, related to their specific situation. The questions ‘who am I?’, ‘why do I live?’, ‘what is the meaning of my life?’ seem particularly relevant when a person is in a difficult situation. Single parenthood can be perceived as such a situation. The literature of the subject suggests multiple negative consequences of the fact of raising a child by a single parent; these consequences are faced not only by children, but also by adults. It is not possible to state unequivocally that the lack of favourable conditions will deprive a person of their sense of life purpose and meaning entirely. Similarly, even when objectively favourable conditions exist, they are not a guarantee that a person will perceive her or his life as meaningful. In a woman’s life, a difficult situation such as being a single mother, combined with homelessness and a necessity to move to a residential home for single mothers, can become the stimulus to search for and experience the meaning of life or, on the contrary, it can be a source of an existential insecurity. The purpose of this research was to learn how single mothers perceive the meaning of their lives and to assess their life purposes and aspirations. The theoretical context of these reflections is based on K. Popielski’s concept of the noetic dimension of personality and existence. The research was conducted among women living in residential homes for single mothers. Their sense of purpose and meaning in life was assessed with the use of J.C. Crumbaugh and L.T. Maholick’s Purpose in Life Scale (PIL. The collected material was evaluated using quantitative analysis.

  12. Housing Dependence and Intimate Relationships in the Lives of Low-Income Puerto Rican Mothers

    Science.gov (United States)

    Clark, Sherri Lawson; Burton, Linda M.; Flippen, Chenoa A.

    2011-01-01

    Using longitudinal ethnographic data from the Three-City Study, the authors examined the relationship between 16 low-income Puerto Rican mothers' housing dependencies and their intimate partner relations. This study traced mothers' dependent housing arrangements and entree to marital or cohabiting relationships from their teens through their…

  13. Relationships between psychosocial factors and abusive parenting attitudes in low-income single mothers.

    Science.gov (United States)

    Lutenbacher, Melanie

    2002-01-01

    Linkages among family violence, maternal mental health, and parenting attitudes are not clearly understood. To investigate the relationships of abuse (childhood and/or partner), everyday stressors, self-esteem, depressive symptoms, and anger with abusive parenting attitudes. Cross-sectional analysis of data collected in interviews with 53 low-income, single mothers from wave two of a 3-wave study. A conceptual framework and bivariate correlations guided a series of multiple regressions to identify the best predictors for each variable. A high prevalence of abuse, depressive symptoms, and abusive parenting attitudes was found. Few women had ever received mental health treatment. Abuse (partner and childhood physical) predicted higher everyday stressors which in turn predicted lower self-esteem. Childhood abuse and lower self-esteem predicted more depressive symptoms. More depressive symptoms were related to higher levels of state anger. More everyday stressors and more depressive symptoms predicted higher levels of trait anger. Higher levels of anger expression were associated with higher everyday stressors and lower self-esteem. The presence of partner abuse best predicted higher levels of overall abusive parenting attitudes and more parent-child role reversal. Less parental empathy was associated with higher levels of state anger. This study partially explains the relationships of maternal abuse history and mental health status with parenting attitudes. Other predictors of parenting attitudes remain to be identified. The findings underscore the need for healthcare providers to consider the mental health status and abuse histories of low-income, single mothers. The potential disturbance in the parenting process of single mothers in abusive relationships warrants further investigation.

  14. Single Electron Transfer Living Radical Polymerization via a New Initiator

    Science.gov (United States)

    Bai, Xiongxiong; Hu, Ying; Zhang, Xu; Ai, Lingling; Cheng, Chuanjie

    2014-08-01

    Research and development of novel initiating system such as single electron transfer living radical polymerization (SET-LRP) is of high importance in polymer chemistry. A new SET-LRP initiator was synthesized and applied to prepare end-functionalized poly(methyl methacrylate) (PMMA) in this study. α-Trichloromethyl benzyl alcohol was firstly synthesized, followed by preparation of PMMA under SET-LRP conditions. Conversion of MMA was 81.9%, and the molecular weight of PMMA was about 2.5 kDa at 60 °C for 1 h. Consistency of the number-average molecular weight of PMMA from NMR, GPC and theoretical calculation indicated that the polymerization featured controllable property. Broad molecular weight distribution (MWD) may be ascribed to branched polymers formed by initiation and chain transfer.

  15. Single Electron Transfer Living Radical Polymerization via a New Initiator

    International Nuclear Information System (INIS)

    Bai, Xiongxiong; Hu, Ying; Zhang, Xu; Ai, Lingling; Cheng, Chuanjie

    2014-01-01

    Research and development of novel initiating system such as single electron transfer living radical polymerization (SET-LRP) is of high importance in polymer chemistry. A new SET-LRP initiator was synthesized and applied to prepare end-functionalized poly(methyl methacrylate) (PMMA) in this study. α-Trichloromethyl benzyl alcohol was firstly synthesized, followed by preparation of PMMA under SET-LRP conditions. Conversion of MMA was 81.9%, and the molecular weight of PMMA was about 2.5 kDa at 60 °C for 1 h. Consistency of the number-average molecular weight of PMMA from NMR, GPC and theoretical calculation indicated that the polymerization featured controllable property. Broad molecular weight distribution (MWD) may be ascribed to branched polymers formed by initiation and chain transfer

  16. Living with the unknown: Posttraumatic stress disorder in pediatric bone marrow transplantation survivors and their mothers.

    Science.gov (United States)

    Taskıran, Gülseren; Sürer Adanır, Aslı; Özatalay, Esin

    2016-04-01

    Bone marrow transplantation (BMT) is used to treat children with various hematologic, oncologic, and metabolic diseases. Although the treatment can be lifesaving, it is also physically and psychologically demanding for both the child and caregivers. In previous studies, BMT is found to be related with anxiety, posttraumatic stress disorder (PTSD), depression, and psychosocial problems both in children and parents. The aim of this study was to investigate PTSD in pediatric BMT survivors and their mothers compared with the healthy controls. Twenty-seven BMT survivors and their mothers and 28 healthy peers and their mothers were recruited as the study group and as the comparison group, respectively. All children were interviewed using Child Posttraumatic Stress Disorder-Reaction Index (CPTSD-RI) for assessing posttraumatic stress responses. As for mothers, Clinician-Administered PTSD Scale (CAPS) was used. In healthy children and mothers, instead of BMT, the most important traumatic event reported by them was included. All data were analyzed by a neutral statistician from the Department of Biostatistics of the university. The BMT group, both children and mothers, obtained significantly higher PTSD rates than the control group (66.5% and 17.8%, respectively, in children; 57.6% and 7%, respectively, in mothers). However, there was a weak correlation between survivors' and mothers' posttraumatic stress responses. These findings suggest that BMT is a significant stressor for both children and mothers. Clinicians should be aware of psychiatric symptoms of children who underwent such a life-threatening condition. Combination of medical treatment with psychosocial support is imperative.

  17. Cohabitational effect of grandparents on dietary intake among young Japanese women and their mothers living together. A multicenter cross-sectional study.

    Science.gov (United States)

    Kobayashi, Satomi; Asakura, Keiko; Suga, Hitomi; Sasaki, Satoshi

    2015-08-01

    We examined the cohabitational effect of the grandparents on dietary intakes among young Japanese women and their mothers, using data from a cross-sectional multicenter study conducted in 35 of 47 prefectures in Japan. Among a total of 2032 three-generation families, 1336 female dietetic students (18-20 years), 1336 of their mothers (36-59 years), and 1560 of their paternal or maternal grandmothers (59-94 years) were included. Intake of foods and nutrients was assessed with self-administered diet history questionnaires. The prevalence of students and their mothers living with the grandparents (GP) was 36%. Students living with GP had a higher intake of fruits, vegetables, total dietary fiber, β-carotene, vitamin C and potassium and lower intake of meats than those living without GP. For mothers, fish and shellfish and vitamin C intakes were higher and meat intake was lower among participants living with GP. The median correlation coefficients of food and nutrient intakes in the students-grandmothers living together was higher than in those living apart for both paternal grandmothers (PGM) and maternal grandmothers (MGM) (all P <0.01). The median values of mothers-PGM living together were also significantly higher than in those living apart (P <0.01). However, the values of mothers-MGM did not significantly differ between those living with and without MGM. These results may suggest that family members who live together tend to share similar dietary habits. Further, the mother's dietary habits might affect those of their children even after they live apart. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. “Raising Him … to Pull His Own Weight”: Boys’ Household Work in Single- Mother Households

    Science.gov (United States)

    Berridge, Clara W.; Romich, Jennifer L.

    2012-01-01

    In this study, the authors examine boys’ household work in low- and moderate-income single-mother families. Through describing the work that boys do, why they do this work, and the meaning that they and their mothers give to this work, they add to the understanding of housework as an arena for gender role reproduction or interruption. Their data reveal that adolescent boys did a significant amount of work and took pride in their competence. Mothers grounded their expectations of boys’ household contributions in life experience. They both needed their sons’ day-to-day contributions and wanted their sons to grow into men who were competent around the house and good partners. In demanding household work from their sons, these single mothers themselves work to undermine the traditional gendered division of such labor. PMID:22773881

  19. African-American Women Who Use Crack Cocaine: A Comparison of Mothers Who Live with and Have Been Separated from Their Children

    Science.gov (United States)

    Lam, W.K.K.; Wechsberg, W.; Zule, W.

    2004-01-01

    Objective:: This study examined factors that influenced caregiver status for African-American mothers who use crack cocaine but are not receiving drug treatment and participated in an HIV prevention study in North Carolina. Method:: Caregiver mothers who were living with at least one of their children at intake (n=257) were compared with…

  20. Adult outcomes of teen mothers across birth cohorts

    Directory of Open Access Journals (Sweden)

    Anne Driscoll

    2014-04-01

    Full Text Available Background: Teen and young adult mothers have lower socioeconomic status than older mothers. Objective: This study analyzes the socioeconomic status (SES of teen, young adult, and older adult mothers across four five-year birth cohorts from 1956 to 1975 who were teens from 1971 to 1994. Methods: Data were pooled from the 1995, 2002, and 2006-2010 National Survey of Family Growth (NSFG. Mothers were categorized by age at first birth and by their birth cohorts. The SES (education, single motherhood, poverty, employment of teen, young adult, and older mothers was compared across cohorts and within cohorts. Results: Among teen mothers, the odds of fulltime employment improved across birth cohorts and the odds of educational attainment beyond high school did not vary. Their odds of single motherhood and living in poverty increased across cohorts. The odds of higher education and single motherhood increased across birth cohorts for young adult mothers as did the odds of living in poverty, even if working fulltime. Among older adult mothers, educational attainment and the odds of single motherhood rose for recent cohorts. Conclusions: Comparisons between teen mothers and both young adult and all adult mothers within cohorts suggest that gaps in single motherhood and poverty between teen and adult mothers have widened over time, to the detriment of teen mothers. Teen mothers have become more likely to be single and poor than in the past and compared to older mothers.

  1. Challenges of the education of children living with their mothers in prisions: the function of the University and public power

    Directory of Open Access Journals (Sweden)

    Andrea Cristiane Maraschin Bruscato

    2015-05-01

    Full Text Available This article presents the care project for infant development, considering babies who are living with their mothers at the penitential institution, Madre Pelletier Female Penitentiary, in Rio Grande do Sul State. The project promotes actions to secure the Physical well-being, affective and social cognitive among 3 to 12 months children, into the perspective of taking care of and educate them. Pedagogy students, under the professors’ supervision and orientation in their courses, plan and develop educational activities twice a week, considering the particularities of prison’s routine and the babies’ development needs. However, it is necessary to recognize the lack of public policies responsible with the children development necessities in order to ensure that the connection between mother and child are established by the healthiest way.

  2. What is a family? Kinship, reproductive practices and single mothers by choice

    DEFF Research Database (Denmark)

    Ravn, Tine

    The proliferation of assisted reproductive technologies (ARTs) such as in vitro fertilization (IVF) has influenced and facilitated the rise of ‘new’ kinship practices and family formations. Furthermore, these technologies – in obscuring the relation between ‘biological facts of conception...... of single mothers by choice, the paper explores how the women conceive and enact the notions of family and kinship in terms of creating significant relations and networks. Furthermore, the paper shows and discusses how a complex interplay of biogenetic and social ties impacts on these family conceptions......, showing that the application of ARTs is also fashioned by more traditional notions of kinship (Levine 2008; Thompson 2005) and that the nuclear family ideal remains a strong notion within the realms of reproduction and parenting (Cutas & Chan 2012:5). By contributing to this continuing question of how...

  3. What is a family? Kinship, reproductive practices and single mothers by choice

    DEFF Research Database (Denmark)

    Ravn, Tine

    The proliferation of assisted reproductive technologies (ARTs) such as in vitro fertilization (IVF) has influenced and facilitated the rise of ‘new’ kinship practices and family formations. Furthermore, these technologies – in obscuring the relation between ‘biological facts of conception...... of single mothers by choice, the paper explores how the women conceive and enact the notions of family and kinship in terms of creating significant relations and networks. Furthermore, the paper shows and discusses how a complex interplay of biogenetic and social ties impacts on these family conceptions...... and the social categories of kinship’ – have challenged existing normative ideas about family constructions and kinship conceptualizations (Edwards & Salazar 2009:11; Levine 2008; Melhuus 2012). Meanwhile, others have questioned the degree to which our understandings of biogenetic relations have been revised...

  4. Days of Their Lives: Reflections on Adolescent Girls and Adolescent Mothers.

    Science.gov (United States)

    Victor, Susan M.

    1992-01-01

    Reviews literature on issues of sexuality for adolescent girls, interweaving quotes from adolescent mothers. Information comes from the author's work educating teenage parents. The article focuses on the relationship of social pressures to sexuality, femininity, the body, silence and rebellion, adolescent motherhood, and sexual abuse. (SM)

  5. Stress, Social Resources, and Depression among Never-Married and Divorced Rural Mothers

    Science.gov (United States)

    Turner, Heather A.

    2006-01-01

    While the impact of single parenting on women and children has long been a concern, very little research has focused on single parents living in rural areas. Based on a probability sample of 508 single mothers aged 18-39 living in rural Northern New England, the present study: (1) examines the impact of several domains of stress on mothers'…

  6. Increased health risks of children with single mothers: the impact of socio-economic and environmental factors.

    Science.gov (United States)

    Scharte, Marion; Bolte, Gabriele

    2013-06-01

    Adverse effects of single parenthood on children's health have been reported before. Socio-economic difficulties are discussed as mediating factors. As child health also depends on environmental conditions, we investigated the impact of environmental exposures and socio-economic factors on differences in health outcomes of children with single mothers vs. couple families. Data on 17,218 pre-school children (47% female) from three cross-sectional surveys conducted during 2004-07 in Germany were analysed. Health and exposure assessment were primarily based on parental report. Effects of socio-economic indicators (maternal education, household income) and environmental factors (traffic load at the place of residence, perceived environmental quality) on associations of four health outcomes (parent-reported health status, asthma, overweight, psychological problems) with single parenthood were determined by logistic regression analyses. Children with single mothers showed an increased risk regarding parent-reported poor health status [boys: odds ratio (OR) 1.39 (95% confidence interval (CI): 1.06-1.82), girls: 1.73 (1.28-2.33)], psychological problems [boys: 1.90 (1.38-2.61), girls: 1.58 (1.03-2.42)], overweight [only boys: OR 1.23 (1.01-1.50) and asthma [only girls: OR 1.90 (1.15-3.15)]. Adjusting for socio-economic factors attenuated the strength of the association of family type with child health. Although environmental factors were associated with most health outcomes investigated and children of single mothers were more often exposed, these environmental factors did not alter the differences between children with single mothers and couple families. The increased health risks of children from single-mother families vs. couple families are partly explained by socio-economic factors, but not by the environmental exposures studied.

  7. Dioxins and Nonortho PCBs in Breast Milk of Vietnamese Mothers Living in the Largest Hot Spot of Dioxin Contamination.

    Science.gov (United States)

    Nghi, Tran Ngoc; Nishijo, Muneko; Manh, Ho Dung; Tai, Pham The; Van Luong, Hoang; Anh, Tran Hai; Thao, Pham Ngoc; Trung, Nguyen Viet; Waseda, Tomoo; Nakagawa, Hideaki; Kido, Teruhiko; Nishijo, Hisao

    2015-05-05

    Bien Hoa Air Base is the largest dioxin contamination hot spot in Vietnam. In 2012, we recruited 216 mothers who were living in 10 communities around Bien Hoa Air Base and had delivered newborns at a prefecture hospital, and we investigated recent exposure levels of dioxins and nonortho PCBs in their breast milk. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-tetraCDD) was present at 2.6 pg/g lipid in primiparae and 2.2 pg/g lipid in multiparae. Among multiparae and total subjects, significant high prevalence of 2,3,7,8-tetraCDD≥5 pg/g lipid and 2,3,7,8-tetraCDD contribution≥40% were observed in mothers living in the five communities closest to Bien Hoa Air Base. The TEQ for nonortho PCBs was 1.6 pg-TEQ/g lipid for primiparae, and this was even lower than that in the unsprayed area. The length of residency was a strong factor to increase dioxins, including 2,3,7,8-tetraCDD. Residency in the five communities with the highest exposure was a specific risk factor for increased 2,3,7,8-tetraCDD in breast milk. Food intake might contribute partly to the increased levels of dioxin congeners other than 2,3,7,8-tetraCDD in breast milk. These results suggest that Bien Hoa Air Base has led to elevated 2,3,7,8-tetraCDD levels in breast milk of mothers in nearby areas even in the recent years.

  8. Intervening on conflict, parental bonds, and sexual risk acts among adolescent children of mothers living with HIV.

    Directory of Open Access Journals (Sweden)

    Mary Jane Rotheram-Borus

    Full Text Available In 1993-1994, a psychosocial intervention conducted in New York City significantly improved outcomes for parents living with HIV and their adolescent children over six years. We examine if the intervention benefits are similar for adolescents of mothers living with HIV (MLH in 2004-2005 in Los Angeles when MLH's survival had increased substantially.Adolescents of MLH in Los Angeles (N = 256 aged 12-20 years old were randomized with their MLH to either: 1 a standard care condition (n = 120 adolescent-MLH dyads; or 2 an intervention condition consisting of small group activities to build coping skills (n = 136 adolescent-MLH dyads, 78% attended the intervention. At 18 months, 94.7% of adolescents were reassessed. Longitudinal structural equation modeling examined if intervention participation impacted adolescents' relationships with parents and their sexual risk behaviors.Compared to the standard care, adolescents in the intervention condition reported significantly more positive family bonds 18 months later. Greater participation by MLH predicted fewer family conflicts, and was indirectly associated with less adolescent sexual risk behavior at the 18 month follow-up assessment. Anticipated developmental patterns were observed--sexual risk acts increased with age. Reports were also consistent with anticipated gender roles; girls reported better bonds with their mothers at 18 months, compared to boys.Adolescents of MLH have better bonds with their mothers as a function of participating in a coping skills intervention and reduced sexual risk-taking as a function of MLH intervention involvement.

  9. Poverty, Public Assistance and Single Mothers : A Comparative Look at Norway, Russia and the U.S

    Directory of Open Access Journals (Sweden)

    Pamela A. Brown

    2015-03-01

    Full Text Available This article presents some of the perceptions and experiences of mothers on public assistance and their social workers in three countries—Norway, Russia and the US. Based on in-depth interviews in each country with social workers and the women, five themes emerged that will be presented here. The mothers spoke of the inadequacy of economic support to meet their needs, a life of poverty that sometimes involved discrimination of them as mothers, and a loss of hope that their lives could be different. Two themes that stood out in the interviews with social workers was the lack of resources needed to help their clients and the dilemma faced as an agent of social welfare institutions balanced with being an advocate for the mothers’ needs.

  10. Age-Related Parenting Education: Model Development and Application to an Emerging Family Constellation - Single-by-Choice Mothers.

    Science.gov (United States)

    Holle, Kimberly Ann

    The purpose of this paper is twofold: (1) to describe an adaptation of the Schaefer Circumplex Model to age-related parenting theory and techniques, and (2) to illustrate its application in relation to the emerging numbers of single-by-choice mothers. The method described superimposes both a child's and a parent's cognitive and psychosocial…

  11. Seeking Emancipation from Gender Regulation: Reflections on Home Space for a Black Woman Academic/Single Mother

    Science.gov (United States)

    William-­White, Lisa

    2012-01-01

    Using the work of Judith Butler on gender regulation, Black Feminist Thought (BFT), and autobiographic storytelling, this piece illustrates how essentialist notions of gender, and discourses related to gender create conflict in shaping identity construction for a Black woman academic and single mother (BWA/SM) in the United States. This piece…

  12. The lived experience of feeling loved: a study of mothers in a parolee program.

    Science.gov (United States)

    Baumann, S L

    2000-10-01

    The purpose of this study was to uncover the meaning of the lived experience of feeling loved. The site of this study was a shelter-based parolee program. The framework that guided the study was Parse's human becoming theory, and the method was Parse's research methodology. The finding of this study is the lived experience of feeling loved is an unshakable presence arising with moments of uplifting delight amid bewildering trepidation. The findings integrated into the human becoming theory show the paradoxical and dialectic nature of feeling loved. Feeling loved is linked to living freedom, trust, and hope.

  13. Factors influencing exposure to secondhand smoke in preschool children living with smoking mothers.

    Science.gov (United States)

    Mills, Lynsey M; Semple, Sean E; Wilson, Inga S; MacCalman, Laura; Amos, Amanda; Ritchie, Deborah; O'Donnell, Rachel; Shaw, April; Turner, Stephen W

    2012-12-01

    The health effects on young children of exposure to secondhand smoke (SHS) are well described. Recent work suggests that over one quarter of school-aged children in Scotland are regularly exposed to SHS in the home. The study was designed to describe SHS exposure in preschool children whose mothers smoked and identify factors that influence exposure. Smoking mothers with at least one child aged 1-5 years were recruited to the Reducing Families' Exposure to Secondhand Smoke in the Home study. Concentrations of airborne particulate matter less than 2.5 μm in size (PM(2.5)) in the home were measured together with child's salivary cotinine. Demographics including age, accommodation type, socioeconomic status, and number of cigarettes smoked at home were recorded. Data were collected from 54 homes. In 89% of the homes, concentrations of PM(2.5) exceeded health-based guidance values at some point of the day. Household PM(2.5) concentrations were highest during the evening hours of 6 p.m. to midnight. Younger children had higher salivary cotinine concentrations than older children, and the geometric mean of salivary cotinine was 2.36 ng/ml. Household smoking restrictions and maternal confidence in enforcing smoking restrictions in their own home were strongly associated with child's SHS exposure. Preschool children's exposure to SHS in homes where the mother smokes is considerable. Interventions and policy development to increase parental awareness of the health effects of SHS and provide parents with the confidence to implement smoke-free households are required to reduce the SHS exposure of preschool age children.

  14. What helps children to be more active and less sedentary? Perceptions of mothers living in disadvantaged neighbourhoods.

    Science.gov (United States)

    Veitch, J; Hume, C; Salmon, J; Crawford, D; Ball, K

    2013-01-01

      Increasing children's participation in physical activity and decreasing time spent in sedentary behaviours is of great importance to public health. Despite living in disadvantaged neighbourhoods, some children manage to engage in health-promoting physical activity and avoid high levels of screen-based activities (i.e. watching TV, computer use and playing electronic games). Understanding how these children manage to do well and whether there are unique features of their home or neighbourhood that explain their success is important for informing strategies targeting less active and more sedentary children. The aim of this qualitative study was to gain in-depth insights from mothers regarding their child's resilience to low physical activity and high screen-time.   Semi-structured face-to-face interviews were conducted with 38 mothers of children who lived in disadvantaged neighbourhoods in urban and rural areas of Victoria, Australia. The interviews were designed to gain in-depth insights about perceived individual, social and physical environmental factors influencing resilience to low physical activity and high screen-time.   Themes relating to physical activity that emerged from the interviews included: parental encouragement, support and modelling; sports culture in a rural town; the physical home and neighbourhood environment; child's individual personality; and dog ownership. Themes relating to screen-time behaviours encompassed: parental control; and child's individual preferences.   The results offer important insights into potential avenues for developing 'resilience' and increasing physical activity and reducing screen-time among children living in disadvantaged neighbourhoods. In light of the negative effects of low physical activity and high levels of screen-time on children's health, this evidence is urgently needed. © 2011 Blackwell Publishing Ltd.

  15. Physical chemistry in a single live cell: confocal microscopy.

    Science.gov (United States)

    Amin, Md Asif; Nandi, Somen; Mondal, Prasenjit; Mahata, Tanushree; Ghosh, Surajit; Bhattacharyya, Kankan

    2017-05-24

    A live cell is a complex, yet extremely important container. Understanding the dynamics in a selected intracellular component is a challenging task. We have recently made significant progress in this direction using a confocal microscope as a tool. The smallest size of the focused spot in a confocal microscope is ∼0.2 μm (200 nm). This is nearly one hundred times smaller than the size of a live cell. Thus, one can selectively study different intracellular components/organelles in a live cell. In this paper, we discuss how one can image different intracellular components/organelles, record fluorescence spectra and decay at different locations, ascertain local polarity and viscosity, and monitor the dynamics of solvation, proton transfer, red-ox and other phenomena at specified locations/organelles inside a cell. We will highlight how this knowledge enriched us in differentiating between cancer and non-cancer cells, 3D tumor spheroids and towards drug delivery.

  16. Health insurance tax credits, the earned income tax credit, and health insurance coverage of single mothers.

    Science.gov (United States)

    Cebi, Merve; Woodbury, Stephen A

    2014-05-01

    The Omnibus Budget Reconciliation Act of 1990 enacted a refundable tax credit for low-income working families who purchased health insurance coverage for their children. This health insurance tax credit (HITC) existed during tax years 1991, 1992, and 1993, and was then rescinded. A difference-in-differences estimator applied to Current Population Survey data suggests that adoption of the HITC, along with accompanying increases in the Earned Income Tax Credit (EITC), was associated with a relative increase of about 4.7 percentage points in the private health insurance coverage of working single mothers with high school or less education. Also, a difference-in-difference-in-differences estimator, which attempts to net out the possible influence of the EITC increases but which requires strong assumptions, suggests that the HITC was responsible for about three-quarters (3.6 percentage points) of the total increase. The latter estimate implies a price elasticity of health insurance take-up of -0.42. Copyright © 2013 John Wiley & Sons, Ltd.

  17. Seeking Emancipation from Gender Regulation: Reflections on Home space for a Black Woman Academic/ Single Mother

    Directory of Open Access Journals (Sweden)

    Lisa William-­White

    2012-06-01

    Full Text Available Using the work of Judith Butler on gender regulation, Black Feminist Thought (BFT, and autobiographic storytelling, this piece illustrates how essentialist notions of gender, and discourses related to gender create conflict in shaping identity construction for a Black woman academic and single mother (BWA/SM in the United States. This piece reveals complex gendered and racialized tropes related to notions of motherhood and womanhood, particularly within the author’s own family. Included here is how the author attempts to transcend these complexities in her quest for self­definition and self­actualization, unbridled by gender norms. Yet, race, gender and parental status are significant intersecting categories in identity construction, andinherent in the constructions are hegemonic discourses with which the author continues to grapple. Consequently, the struggle to transcend these forces is further complicated by the limited representation of Black women in the US academy, and by the types of academic work where they find themselves typically situated.

  18. Psychometric properties of the Rosenberg self-esteem scale in African American single mothers.

    Science.gov (United States)

    Hatcher, Jennifer; Hall, Lynne A

    2009-02-01

    The Rosenberg Self-Esteem (RSE) Scale is a commonly used measure of global self-esteem, an important element of mental health. The purpose of this cross sectional secondary analysis was to examine the psychometric properties of the scale in a sample of 98 African American single mothers. The RSE Scale showed adequate internal consistency with an alpha coefficient of .83. Two factors that accounted for a total of 54.7% of the variance were extracted. Self-esteem showed a strong negative relationship with both depressive symptoms and negative thinking. This study provides support for the internal consistency of the RSE Scale and partial support for its construct validity in this population. The RSE appears to represent a bidimensional construct of self-esteem for African American women, with the cultural influences of racial esteem and the rejection of negative stereotypes forming a separate and distinct aspect of this concept. The RSE Scale should be used and interpreted with caution in this population given these findings.

  19. Single-molecule fluorescence microscopy in living Caenorhabditis elegans

    NARCIS (Netherlands)

    van Krugten, Jaap; Peterman, Erwin J.G.

    2018-01-01

    Transportation of organelles and biomolecules is vital for many cellular processes. Single-molecule (SM) fluorescence microscopy can expose molecular aspects of the dynamics that remain unresolved in ensemble experiments. For example, trajectories of individual, moving biomolecules can reveal

  20. Adolescent Mothers in a Transitional Living Facility: An Exploratory Study of Support Networks and Attachment Patterns

    Science.gov (United States)

    Schwartz, Ann E.; McRoy, Ruth G.; Downs, A. Chris

    2004-01-01

    Most of the research literature on attachment and adolescent transitions has addressed youth in family settings. This article explores these issues with a sample of 25 pregnant and parenting teens living in a transitional shelter. Using case records and interview data as well as results of standardized measures of depression, self-esteem, child…

  1. Revisiting an era in Germany from the perspective of adolescents in mother-headed single-parent families.

    Science.gov (United States)

    Sharma, Deepali; Silbereisen, Rainer K

    2007-02-01

    Much of the documented work on families headed by single mothers is based on empirical evidence from North America and a few Anglo-Saxon countries. Many researchers consider single-mother families to be at a disadvantage because of nonsupportive family policies. This paper uses data from a social context that differed hugely in this respect-the German Democratic Republic (GDR). The GDR provided extensive state support to single-mother families and, thus, was vastly different from other countries. Based on a literature review and using Hill's family stress theory, this paper is based on the hypothesis that adolescents living in mother-headed single-parent families and those living with their biological parents would have near-similar social outcomes (display of psychosomatic symptoms, perception of stress, display of delinquent behaviour, life satisfaction, academic self-efficacy, and academic grades) and family-related outcomes (relationship with parents and perception of family environment). Further, it was hypothesized that adolescents from stepfather families would display more negative social and family-related outcomes when compared with respondents from the other two family types. The sample comprised 1302 adolescents (M  =  13.82 years, SD  =  1.88) who were recruited from the city of Leipzig in the year immediately following German unification, 1991. Respondents reported on measures of psychosomatic symptoms, stress, delinquency, life satisfaction, academic achievement, and family-related variables. ANCOVAs, with a control for income adjusted for household size, indicated adolescents from single-mother families to have very similar experiences to respondents living with their biological parents on all measures except for their assessment of family environment. Adolescents living in stepfather families reported the least favourable experiences. Results are indicative of a social context in Germany that, despite unification and the early hardships for

  2. Living with the loss of a child: mothers in the criminal justice system.

    Science.gov (United States)

    Lewin, Linda C; Farkas, Kathleen J

    2012-12-01

    Bereaved mothers have expressed the need to maintain a bond with their children who have died, to retain control over the funeral rituals, and to express their loss to others who are empathetic. This study describes grief over the loss of a child in women who have been or are currently incarcerated, and the influence of the women's family members. This descriptive qualitative study consisted of open-ended interview questions to encourage the women to describe their experience in their own words. A purposive sample (N = 10) was recruited from a prison re-entry program and a county jail. All of the participants described maladaptive responses such as the use of alcohol and drugs, restricted family support, and dysfunctional coping. None of the participants had received focused bereavement services. The descriptive perspective from the participants can inform clinicians who work with women who have a history of involvement with the criminal justice system, and recommends that they should inquire about the women's children and experiences with loss. Community re-entry and jail/prison counselors should provide access to confidential and group-based therapeutic support, including Compassionate Friends.

  3. Homelessness Pathways for Australian Single Mothers and Their Children: An Exploratory Study

    OpenAIRE

    Wayne Warburton; Elizabeth Whittaker; Marina Papic

    2018-01-01

    There is increasing concern about family homelessness. Homeless mothers and their children are one of society’s most disadvantaged and at-risk populations. However, very little Australian research exploring mothers’ views on their homelessness experiences exists. Using semi-structured interviews with 14 mothers and four agency staff, this study explored homeless Australian mothers’ pathways into and out of homelessness, their specific needs and the services and supports that were (or would ha...

  4. HIV-related stigma in pregnancy and early postpartum of mothers living with HIV in Ontario, Canada.

    Science.gov (United States)

    Ion, Allyson; Wagner, Anne C; Greene, Saara; Loutfy, Mona R

    2017-02-01

    HIV-related stigma is associated with many psychological challenges; however, minimal research has explored how perceived HIV-related stigma intersects with psychosocial issues that mothers living with HIV may experience including depression, perceived stress and social isolation. The present study aims to describe the correlates and predictors of HIV-related stigma in a cohort of women living with HIV (WLWH) from across Ontario, Canada during pregnancy and early postpartum. From March 2011 to December 2012, WLWH ≥ 18 years (n = 77) completed a study instrument measuring independent variables including sociodemographic characteristics, perceived stress, depression symptoms, social isolation, social support and perceived racism in the third trimester and 3, 6 and 12 months postpartum. Multivariable linear regression was employed to explore the relationship between HIV-related stigma and multiple independent variables. HIV-related stigma generally increased from pregnancy to postpartum; however, there were no significant differences in HIV-related stigma across all study time points. In multivariable regression, depression symptoms and perceived racism were significant predictors of overall HIV-related stigma from pregnancy to postpartum. The present analysis contributes to our understanding of HIV-related stigma throughout the pregnancy-motherhood trajectory for WLWH including the interactional relationship between HIV-related stigma and other psychosocial variables, most notably, depression and racism.

  5. The sexual lives of medical students: a single institution survey.

    Science.gov (United States)

    Shindel, Alan W; Ferguson, Genoa G; Nelson, Christian J; Brandes, Steven B

    2008-04-01

    Little is known about the personal sexual lives of medical students. To assess sexual habits and determine the presence of sexual dysfunction among medical students. Demographic and sexual experience data and domain scores on validated sexuality surveys. Instruments selected included the International Index of Erectile Function, the Index of Premature Ejaculation, and the Self Esteem and Relationship Quality Survey for male medical students and the Female Sexual Function Index (FSFI), and the Index of Sex Life for female medical students. Medical students at our institution were invited to complete a demographic questionnaire and gender- appropriate sexuality surveys. Results were compared with established normative data and validated cut-off scores when available. Linear regression and Pearson coefficient were used to assess relationships between variables. There were 132 responses (78 female mean age 24 years, 54 male, mean age 25 years). Condoms and oral contraceptives were the most popular form of contraception. Among men, 81.5%, 37%, and 93% were in a relationship, married, and heterosexual, respectively. Among women, 64%, 18%, and 95% were in a relationship, married, and heterosexual. Erectile dysfunction was reported by 30% of men. Proxy measures of other sexual problems in men revealed a 28% prevalence of dissatisfaction with sex life, a 28% prevalence of problems controlling ejaculation, an 11% prevalence of orgasmic dysfunction, and a 6% prevalence of low sexual desire. Based on validated FSFI scoring, 63% of women were at high risk of sexual dysfunction. Proxy measures of other problems in women indicated disorders of pain, orgasms, desire, sex satisfaction, lubrication, and arousal in 39%, 37%, 32%, 28%, 26%, and 24% of female respondents, respectively. These data provide insight into the sexual lives of medical students. Rates of sexual dysfunction are higher than expected based on normative data. Further research is required.

  6. Attitudes and Beliefs of African Immigrant Mothers Living in the US Towards Providing Comprehensive Sex Education to Daughters Aged 12-17 Years: A Pilot Study.

    Science.gov (United States)

    Agbemenu, Kafuli; Terry, Martha Ann; Hannan, Margaret; Kitutu, Julius; Doswell, Willa

    2016-10-01

    The literature currently contains no comprehensive sex education (CSE) interventions targeting the African immigrant population. African immigrant mothers have been inhibited by several factors from providing their daughters with CSE. The primary aim of this study was to identify attitudes and beliefs of Sub-Saharan immigrant mothers living in the United States towards providing comprehensive sex education to their daughters aged 12-17 years. The study utilized a one-time anonymous nine-question survey. Fifteen women who met the inclusion criteria completed the study survey online or via paper format. African immigrant mothers are willing to allow comprehensive sex to be taught in schools and at home. Accepted education appears to range from religious and moral teaching to some factual information. This research will potentially assist in the designing of more culturally appropriate comprehensive sex education programs for African immigrant mothers and their daughters.

  7. Socioeconomic Status, Parenting, and Externalizing Problems in African American Single-Mother Homes: A Person-Oriented Approach

    Science.gov (United States)

    Anton, Margaret T.; Jones, Deborah J.; Youngstrom, Eric A.

    2016-01-01

    African American youth, particularly those from single-mother homes, are overrepresented in statistics on externalizing problems. The family is a central context in which to understand externalizing problems; however, reliance on variable-oriented approaches to the study of parenting, which originate from work with intact, middle-income, European American families, may obscure important information regarding variability in parenting styles among African American single mothers, and in turn, variability in youth outcomes as well. The current study demonstrated that within African American single-mother families: (a) a person-, rather than variable-, oriented approach to measuring parenting style may further elucidate variability; (b) socioeconomic status may provide 1 context within which to understanding variability in parenting style; and (c) 1 marker of socioeconomic status, income, and parenting style may each explain variability in youth externalizing problems; however, the interaction between income and parenting style was not significant. Findings have potential implications for better understanding the specific contexts in which externalizing problems may be most likely to occur within this at-risk and underserved group. PMID:26053349

  8. Optofluidics for handling and analysis of single living cells

    KAUST Repository

    Perozziello, Gerardo

    2017-12-07

    Optofluidics is a field with important applications in areas such as biotechnology, chemical synthesis and analytical chemistry. Optofluidic devices combine optical elements into microfluidic devices in ways that increase portability and sensitivity of analysis for diagnostic or screening purposes .In fact in these devices fluids give fine adaptability, mobility and accessibility to nanoscale photonic devices which otherwise could not be realized using conventional devices. This review describes several cases inwhich optical or microfluidic approaches are used to trap single cells in proximity of integrated optical sensor for being analysed.

  9. Real-time visualization of intracellular hydrodynamics in single living cells

    NARCIS (Netherlands)

    Potma, Eric O.; Boeij, Wim P. de; Haastert, Peter J.M. van; Wiersma, Douwe A.

    2001-01-01

    Intracellular water concentrations in single living cells were visualized by nonlinear coherent anti-Stokes Raman scattering (CARS) microscopy. In combination with isotopic exchange measurements, CARS microscopy allowed the real-time observation of transient intracellular hydrodynamics at a high

  10. "How can you live without your kids?": Distancing from and embracing the stigma of “incarcerated mother"

    Directory of Open Access Journals (Sweden)

    Brittnie Aiello

    2016-05-01

    Full Text Available This article examines how incarcerated mothers constructed moral identities in the face of stigma. Analyzing data from participant observation and 83 in-depth interviews with incarcerated mothers, we show that mothers claimed moral identities by distancing from the stigma of incarceration and/or embracing the identity of incarcerated mothers. Utilizing these strategies, women challenged the stigma of convicted felon/bad mother and reinforced the assumptions that motherhood is compulsory and should be reserved for women with enough money and standing to give their children advantages. The implications for understanding motherhood as a mechanism of moral identity and social control are discussed.

  11. Children Living with Violence against Their Mothers: The Side Effects on Their Behaviour, Self-Image and School Performance

    Science.gov (United States)

    Koutselini, Mary; Valanidou, Floria

    2014-01-01

    This paper discusses the effects of children's exposure to violence against their mothers. It particularly considers the sided-effects of this violence on the children's behaviour, self-image and school performance. The research indicates that (1) violence against women victimises not only the mothers but also their children, even if the children…

  12. Central dogma at the single-molecule level in living cells.

    Science.gov (United States)

    Li, Gene-Wei; Xie, X Sunney

    2011-07-20

    Gene expression originates from individual DNA molecules within living cells. Like many single-molecule processes, gene expression and regulation are stochastic, that is, sporadic in time. This leads to heterogeneity in the messenger-RNA and protein copy numbers in a population of cells with identical genomes. With advanced single-cell fluorescence microscopy, it is now possible to quantify transcriptomes and proteomes with single-molecule sensitivity. Dynamic processes such as transcription-factor binding, transcription and translation can be monitored in real time, providing quantitative descriptions of the central dogma of molecular biology and the demonstration that a stochastic single-molecule event can determine the phenotype of a cell.

  13. Inequalities in the psychological well-being of employed, single and partnered mothers: the role of psychosocial work quality and work-family conflict

    Directory of Open Access Journals (Sweden)

    Muhajarine Nazeem

    2010-02-01

    Full Text Available Abstract Background A large body of international research reveals that single mothers experience poorer mental health than their partnered counterparts, with socioeconomic disadvantage identified as an important contributory factor in understanding this health disparity. Much less research, however, has focused specifically on the psychological well-being of single mothers who are employed, despite their growing presence in the labor force. Of the research which has considered employment, the focus has been on employment status per se rather than on other important work-related factors which may impact psychological health, such as psychosocial work quality and work-family conflict. The aim of this study was to: (1 compare employed single mothers and employed partnered mothers on measures of psychological distress, psychosocial work quality and work-family conflict; and (2 explore the potential role of work-family conflict and psychosocial work quality as explanations for any observed differences in psychological distress based on partner status. Method Analysis of data obtained from a cross-sectional telephone survey of employed parents in a mid-sized Western Canadian city. Analyses were based on 674 employed mothers (438 partnered and 236 single, who were 25-50 years old, with at least one child in the household. Results Compared to employed single mothers, employed partnered mothers were older, had more education and reported fewer hours of paid work. Single mothers reported higher levels of psychological distress, financial hardship, work-family conflict and poor psychosocial work quality. Statistical adjustment for income adequacy, psychosocial work quality and work-family conflict each independently resulted in single motherhood no longer being associated with psychological distress. Conclusions While single employed mothers did experience higher levels of psychological distress than their partnered counterparts, differences between these

  14. Every death counts: use of mortality audit data for decision making to save the lives of mothers, babies, and children in South Africa.

    Science.gov (United States)

    Bradshaw, Debbie; Chopra, Mickey; Kerber, Kate; Lawn, Joy E; Bamford, Lesley; Moodley, Jack; Pattinson, Robert; Patrick, Mark; Stephen, Cindy; Velaphi, Sithembiso

    2008-04-12

    South Africa is one of the few developing countries with a national confidential inquiry into maternal deaths. 164 health facilities obtain audit data for stillbirths and neonatal deaths, and a new audit network does so for child deaths. Three separate reports have been published, providing valuable information about avoidable causes of death for mothers, babies, and children. These reports make health-system recommendations, many of which overlap and are intertwined with the scarcity of progress in addressing HIV/AIDS. The leaders of these three reports have united to prioritise actions to save the lives of South Africa's mothers, babies, and children. The country is off-track for the health-related Millennium Development Goals. Mortality in children younger than 5 years has increased, whereas maternal and neonatal mortality remain constant. This situation indicates the challenge of strengthening the health system because of high inequity and HIV/AIDS. Coverage of services is fairly high, but addressing the gaps in quality and equity is essential to increasing the number of lives saved. Consistent leadership and accountability to address crosscutting health system and equity issues, and to prevent mother-to-child transmission of HIV, would save tens of thousands of lives every year. Audit is powerful, but only if the data lead to action.

  15. "Is the doctor God to punish me?!" An intersectional examination of disrespectful and abusive care during childbirth against single mothers in Tunisia.

    Science.gov (United States)

    Amroussia, Nada; Hernandez, Alison; Vives-Cases, Carmen; Goicolea, Isabel

    2017-03-04

    Disrespectful and abusive treatment during childbirth is a violation of women's right to dignified, respectful healthcare throughout pregnancy and childbirth. Although reports point out that marginalized groups in society such as single mothers are particularly vulnerable to abusive and disrespectful care, there is a lack of in-depth research exploring single mothers' encounters at the maternal healthcare facilities, especially in Tunisia. In Tunisia, single mothers are particularly vulnerable due to their social stigmatization and socio-economic marginalization. This study examines the self-perceptions and childbirth experiences of single mothers at the public healthcare facilities in Tunisia. This study follows a qualitative design. Eleven single mothers were interviewed in regard to their experiences with maternal healthcare services and their perceptions of the attitudes of the health workers towards them. The interviews also addressed the barriers faced by the participants in accessing adequate maternal healthcare services, and their self-perceptions as single mothers. The data were analyzed using an inductive thematic approach guided by the feminist intersectional approach. Emergent codes were grouped into three final themes. Three themes emerged during the data analysis: 1) Experiencing disrespect and abuse, 2) Perceptions of regret and shame attributed to being a single mother, and 3) The triad of vulnerability: stigma, social challenges, and health system challenges. The study highlights that the childbirth experiences of single mothers are shaped by intersectional factors that go beyond the health system. Gender plays a major role in constructing these experiences while intersecting with other social structures. The participants had experienced disrespectful and discriminatory practices and even violence when they sought maternal healthcare services at the public healthcare facilities in Tunisia. Those experiences reflect not only the poor quality of

  16. A simple optical fiber device for quantitative fluorescence microscopy of single living cells

    NARCIS (Netherlands)

    van Graft, M.; van Graft, Marja; Oosterhuis, B.; Oosterhuis, Bernard; van der Werf, Kees; de Grooth, B.G.; Greve, Jan

    1993-01-01

    simple and relatively inexpensive system is described for obtaining quantitative fluorescence measurements on single living cells loaded with a fluorescent probe to study cell physiological processes. The light emitted from the fluorescent cells is captured by and transported through an optical

  17. Dissecting the Cell Entry Pathway of Dengue Virus by Single-Particle Tracking in Living Cells

    NARCIS (Netherlands)

    van der Schaar, Hilde M.; Rust, Michael J.; Chen, Chen; van der Ende-Metselaar, Heidi; Wilschut, Jan; Zhuang, Xiaowei; Smit, Jolanda M.

    2008-01-01

    Dengue virus (DENV) is an enveloped RNA virus that causes the most common arthropod-borne infection worldwide. The mechanism by which DENV infects the host cell remains unclear. In this work, we used live-cell imaging and single-virus tracking to investigate the cell entry, endocytic trafficking,

  18. Raman tweezers spectroscopy of live, single red and white blood cells.

    Directory of Open Access Journals (Sweden)

    Aseefhali Bankapur

    Full Text Available An optical trap has been combined with a Raman spectrometer to make high-resolution measurements of Raman spectra of optically-immobilized, single, live red (RBC and white blood cells (WBC under physiological conditions. Tightly-focused, near infrared wavelength light (1064 nm is utilized for trapping of single cells and 785 nm light is used for Raman excitation at low levels of incident power (few mW. Raman spectra of RBC recorded using this high-sensitivity, dual-wavelength apparatus has enabled identification of several additional lines; the hitherto-unreported lines originate purely from hemoglobin molecules. Raman spectra of single granulocytes and lymphocytes are interpreted on the basis of standard protein and nucleic acid vibrational spectroscopy data. The richness of the measured spectrum illustrates that Raman studies of live cells in suspension are more informative than conventional micro-Raman studies where the cells are chemically bound to a glass cover slip.

  19. Development and Pilot Testing of 24-Hour Multiple-Pass Recall to Assess Dietary Intake of Toddlers of Somali- and Iraqi-Born Mothers Living in Norway

    Directory of Open Access Journals (Sweden)

    Navnit Kaur Grewal

    2014-06-01

    Full Text Available The aim of this study was to develop, test, and evaluate a 24-h recall procedure to assess the dietary intake of toddlers of Somali- and Iraqi-born mothers living in Norway. A protocol for a 24-h multiple-pass recall procedure, registration forms, and visual tools (a picture library for food identification and portion size estimation was developed and tested in 12 mothers from Somalia and Iraq with children aged 10–21 months. Five female field workers were recruited and trained to conduct the interviews. Evaluation data for the 24-h recall procedure were collected from both the mothers and the field workers. Nutrient intake was calculated using a Norwegian dietary calculation system. Each child’s estimated energy intake was compared with its estimated energy requirement. Both the mothers and the field workers found the method feasible and the visual tools useful. The estimated energy intake corresponded well with the estimated energy requirement for most of the children (within mean ± 2 SD, except for three. The pilot study identified the need for additional foods in the picture library and some crucial aspects in training and supervising the field workers to reduce sources of error in the data collection.

  20. Probing living bacterial adhesion by single cell force spectroscopy using atomic force microscopy

    DEFF Research Database (Denmark)

    Zeng, Guanghong; Ogaki, Ryosuke; Regina, Viduthalai R.

    be considered. We have therefore developed a simple and versatile method to make single-cell bacterial probes for measuring single cell adhesion with atomic force microscopy (AFM).[1] A single-cell probe was readily made by picking up a bacterial cell from a glass surface using a tipless AFM cantilever coated...... with a commercial cell adhesive CellTakTM. The method was applied to four different bacterial strains, and single-cell adhesion was measured on three surfaces (fresh glass, hydrophilic glass, mica). Attachment to the cantilever was stable during the 2 h of AFM force measurements, and viability was confirmed by Live....../Dead fluorescence staining at the end of each experiment. The adhesion force and final rupture length were dependent on bacterial strains, surfaces properties, and time of contact. The single-cell probe offers control of the cell immobilization, thus holds advantages over the commonly used multi-cell probes where...

  1. Gaining confidence and perspective: a phenomenological study of mothers' lived experiences caring for infants at home after neonatal unit discharge.

    Science.gov (United States)

    Murdoch, Marlies R; Franck, Linda S

    2012-09-01

    This paper is a report of a descriptive study of mothers' experiences in caring for preterm or term infants following discharge from a neonatal unit, including how they manage infant pain/discomfort. Few studies have focused on the transition that parents experience after infants are discharged home and little is known about parents' caregiving experiences when their infant has pain/discomfort. Greater knowledge about these issues will help healthcare professionals to better support parents. Nine mothers were interviewed in 2007-2008 about their caregiving experiences in the months following neonatal unit discharge. Interviews were conducted using a descriptive phenomenological approach and analysed using Giorgi's framework. Six themes formed mothers' experiences: apprehension, confidence, responsibility, awareness, normalcy and perspective. Mothers had apprehension about their infants' fragile health, losing support of the neonatal team, and performing medical procedures. They developed confidence as infant health improved. Responsibility for performing infant medical care often dominated mothers' experiences. Awareness of infant needs was described as a learning process utilizing external resources, trial-and-error, and an internal intuitive sense; particularly in identifying and dealing with infant pain/discomfort. During the transition to prior social environments, concerns about normalcy arose. Over time, mothers' gained a philosophical perspective and saw their experiences in a positive light. The apprehension-confidence continuum model provides a framework for nursing assessment of mothers' needs following their infants' discharge from a neonatal unit. Factors such as infant health, medical procedures, and family or health professional support may influence the degree of apprehension or confidence. © 2011 Blackwell Publishing Ltd.

  2. Single-molecule imaging can be achieved in live obligate anaerobic bacteria

    Science.gov (United States)

    Karunatilaka, Krishanthi S.; Coupland, Ben R.; Cameron, Elizabeth A.; Martens, Eric C.; Koropatkin, Nicole K.; Biteen, Julie S.

    2013-02-01

    Single-molecule fluorescence (SMF) permits imaging with nanometer-scale resolution. This technique is particularly useful for cellular imaging as it provides a non-invasive, minimally perturbative means to examine macromolecular localization and dynamics, even in live cells. Here, we demonstrate that nanometer-scale SMF imaging can be extended to a new category of experiments: intracellular imaging of live, obligate anaerobic cells on the benchtop. We investigate the starch-utilization system (Sus) proteins in the gut symbiont Bacteroides thetaiotaomicron and discuss three different labels that we implemented to detect these proteins: fluorescent proteins, the tetracysteine-based FlAsH tag, and the enzymatic HaloTag.

  3. The impact of drugs, infants, single mothers, and relatives on reunification: A Decision-Making Ecology approach.

    Science.gov (United States)

    Wittenstrom, Kim; Baumann, Donald J; Fluke, John; Graham, J Christopher; James, Joyce

    2015-11-01

    Using a Decision-Making Ecology (DME) approach and proportional hazards models, the study isolated four case factor profiles that interacted strongly with race and resulted in disparate reunification outcomes for African American children compared with Anglos. The four interrelated factors were drug involvement, a solo infant case, single mothers, and relative placements. A cohort of 21,763 children from the Texas Department of Family and Protective Services who were placed for the first time in care, who were under 13 and either Anglo or African American were followed for 20 months or more post entry into care. Starting with an initial model consisting of main effects only and consistent with other studies, African American children had a 12% lower hazard rate of reunification compared to Anglo children. However, when a set of case profiles involving combinations of single parents, single infants, drug involvements and kinship placements were crossed with race, the magnitude of the effect of race on hazard rates fanned out from no difference to as much as 68% that of Anglo children. The results show that racial disparities in outcomes resulting from complex, contextual decision making cannot be modeled well with simple main effects models. Copyright © 2015 Elsevier Ltd. All rights reserved.

  4. Assessing the knowledge and attitudes of group of mothers living in Saudi Arabia with regards to their children’s oral health: A cross-sectional study

    Directory of Open Access Journals (Sweden)

    AlBandary AlJameel

    2017-11-01

    Full Text Available Background The knowledge of mothers with respect to health can affect their children’s health either directly by promoting health practices or indirectly by influencing the health-related attitudes and behaviours of children. Aims The aim of this study was to assess the knowledge and attitude of a group of mothers living in Saudi Arabia with regard to their children’s oral health. Methods This cross-sectional study involved 614 mothers living across Saudi Arabia. An electronic web-based questionnaire was developed and distributed among the participants using different social media outlets. Results Almost 80 per cent of study participants were knowledgeable regarding their child’s primary and permanent teeth’s eruption time and agreed that their child’s primary teeth were as important as the permanent teeth. The results also indicated that 79 per cent of the participants were aware that bottle feeding during sleep causes tooth decay and 73.7 per cent knew that the frequency of sugar consumption has a greater impact on oral health than the quantity of sugar consumed. Almost all participants (97 per cent reported that they watch and help their children to brush their teeth. More than half (55.8 per cent of them reported that they take their children for their first dental check-up when the children are one-year old, and almost three-quarters (73 per cent reported they take their children to regular dental check-ups. Conclusion Most mothers had a positive attitude toward their children’s oral health and were reasonably knowledgeable about it; however, further health education is required among some groups and in some aspects particularly those concerning oral and systemic health.

  5. The effect of education based on the theory of planned behavior on preventive behaviors of cutaneous Leishmaniasis in mothers living in endemic city of Natanz

    Directory of Open Access Journals (Sweden)

    MH Baghianimoghadam

    2016-09-01

    Full Text Available Introduction: Due to the limited studies conducted on the educational interventions to change the preventive behaviors of cutaneous leishmaniasis(CL as well as mothers' critical role in creating and maintaining these behaviors, this study aimed to determine the impact of education based on theory of planned behavior on preventive behaviors of CL in mothers living in endemic city of Natanz. Methods: In this case experimental study, two health care centers in endemic areas of CL were randomly assigned into two groups of experimental and control. Using list of mothers covered by each center, 80 patients were selected by simple random sampling, who were required to complete a questionnaire that has been designed based on the theory of planned behavior, and its reliability and validity had been confirmed in the previous studies. Then 4 sessions were held for the experimental group mothers and 2 training sessions were held for people who influenced them, whereas control group received no interventions. Two months after training intervention, the study data were collected again and were analyzed using the SPSS software (ver. 18 via independent statistical t-test, paired t-test, Chi-square and Mann Whitney tests. Results: Before the intervention, no significant differences were observed between the mean scores of different constructs of this theory in the two groups (p>0/005. Though after intervention, a significant increase was observed (p<0/005 in the mean score of knowledge, attitude, subjective norms, perceived behavioral control, intention and action of groups and in control group, only a significant increase was observed in the mean scores of knowledge and attitude (p<0/005. Conclusions: As the findings of the present study revealed, training based on theory of planned behavior can promote preventive behaviors of CL in mothers.

  6. EDUCATIONAL TACTICS OF MOTHERS AND THEIR RELATION TO THE PSYCHOLOGICAL CHARACTERISTICS OF ADOLESCENTS IN TWO-PARENT AND SINGLE-PARENT FAMILIES

    Directory of Open Access Journals (Sweden)

    Константин Борисович Зуев

    2013-04-01

    Full Text Available The paper presents the results of a study of the psychological characteristics of boys and girls from complete and incomplete families. In addition to the type of family, the authors consider the educational tactics of mothers. The combined effects of the type of family and educational tactics on psychological characteristics of the adolescent were investigated. Adolescence was chosen as a period, when on the one hand, a relatively stable personality structure is developed, and on the other hand, the importance of the immediate social environment is extremely high. For our study we selected the psychological characteristics, to the utmost revealing the reaction of children to their parents' divorce: the level of subjective control (degree of responsibility for their own lives, and the sovereignty of the psychological space (clearnesse of psychological boundaries. It is shown that the largest influence on the psychological characteristics of adolescents, regardless of the type of family, is rendered by hostility in maternal education. The dependence of psychological characteristics of maternal directiveness occurs only at high values of such educational tactics that highlights the consistency of the obtained results with the classical studies of single-parent families.DOI: http://dx.doi.org/10.12731/2218-7405-2013-3-6

  7. Unreliable Bodies: A Follow-up Twenty Years Later by a Mother and Daughter about the Impact of Illness and Disability on their Lives.

    Science.gov (United States)

    Weingarten, Kaethe; Worthen, Miranda

    2017-03-01

    We are a mother and daughter, both health care professionals, who offer a follow-up to an article we published twenty years ago about the impact of each other's ongoing, serious medical problems on our relationship. In this article, we contribute a long-term perspective on the differences between having an illness that is well or poorly understood by medical professionals and the lay community. We also discuss health in the context of identity formation and life stage, as during this interval the daughter left home, graduated college, married, and had two children. Also in this period, the mother survived a third breast cancer and other life-threatening illnesses. We discuss the impact of these experiences on each other and in other important relationships in our lives. Current discourses on daughters of breast cancer survivors do not fit our experience and we speculate about why our story differs. We find that although we continue to contend with serious medical issues that impact our own, each other's, and our families' lives, nonetheless, our lives are rich, rewarding, and "appropriate" for our life stage. That is the news. © 2015 Family Process Institute.

  8. Single-Electron Transfer Living Radical Polymerization Platform to Practice, Develop, and Invent.

    Science.gov (United States)

    Lligadas, Gerard; Grama, Silvia; Percec, Virgil

    2017-10-09

    The most fundamental aspects of single-electron transfer (SET) principles are presented. They are discussed according to different definitions used by expert practitioners and are applied to SET living radical polymerization (SET-LRP) according to the definition of the division of organic chemistry of IUPAC that relies on principles elaborated by Taube, Eberson, Chanon, and Kochi. Additional definitions are also discussed to help clarify for the nonexpert contradictory literature reports. Subsequently, the principles and evolution of SET-LRP together with the methodologies currently available to practice it are discussed. It is expected that this Perspective will be able to help experts and nonexperts practice, develop, and invent new concepts and methodologies for SET-LRP to advance its status and the status of other living radical polymerization methods to the level of the most precise living polymerization methods.

  9. “Sex and the Island”: Lives of Single Women in Prince Edward Island

    Directory of Open Access Journals (Sweden)

    Kristie Collins

    2011-05-01

    Full Text Available This article considers the significance attributed to Prince Edward Island in managing a marginalized single female identity, as presented by accounts of thirty never-married and previously-married Island women, aged twenty-seven through sixty-five. As popular media and social narratives overwhelmingly position contemporary single women against an urban backdrop, the question arises as to whether unmarried Island women feel “marooned” in ways their urban counterparts may not. In accordance with feminist aims to produce research for, rather than about, women’s lives, the paper focuses on two themes from fieldwork interviews that were of particular interest to participants. The first theme relates to negotiating female singleness within the Island’s family-centered culture, and the second theme presents participants’ talk around advantages and disadvantages of living in Prince Edward Island, Canada, as single women. The paper concludes with a summary of other findings from the study and suggestions for future research on female singleness and island locales.

  10. Manipulation and Motion of Organelles and Single Molecules in Living Cells

    DEFF Research Database (Denmark)

    Norregaard, Kamilla; Metzler, Ralf; Ritter, Christine M.

    2017-01-01

    driving many cellular processes. The forces on a molecular scale are exactly in the range that can be manipulated and probed with single molecule force spectroscopy. The natural environment of a biomolecule is inside a living cell, hence, this is the most relevant environment for probing their function....... In vivo studies are, however, challenged by the complexity of the cell. In this review, we start with presenting relevant theoretical tools for analyzing single molecule data obtained in intracellular environments followed by a description of state-of-the art visualization techniques. The most commonly...

  11. Probing living bacterial adhesion by single cell force spectroscopy using atomic force microscopy

    DEFF Research Database (Denmark)

    Zeng, Guanghong; Ogaki, Ryosuke; Regina, Viduthalai R.

    Bacteria initiate attachment to the surfaces with the aid of different extracellular polymers. To quantitatively study how these polymers mediate bacterial adhesion and possibly their interactions, it is essential to go down to single cell level, with in mind that cell-to-cell variation should...... with a commercial cell adhesive CellTakTM. The method was applied to four different bacterial strains, and single-cell adhesion was measured on three surfaces (fresh glass, hydrophilic glass, mica). Attachment to the cantilever was stable during the 2 h of AFM force measurements, and viability was confirmed by Live...

  12. Optical computed tomography for spatially isotropic four-dimensional imaging of live single cells.

    Science.gov (United States)

    Kelbauskas, Laimonas; Shetty, Rishabh; Cao, Bin; Wang, Kuo-Chen; Smith, Dean; Wang, Hong; Chao, Shi-Hui; Gangaraju, Sandhya; Ashcroft, Brian; Kritzer, Margaret; Glenn, Honor; Johnson, Roger H; Meldrum, Deirdre R

    2017-12-01

    Quantitative three-dimensional (3D) computed tomography (CT) imaging of living single cells enables orientation-independent morphometric analysis of the intricacies of cellular physiology. Since its invention, x-ray CT has become indispensable in the clinic for diagnostic and prognostic purposes due to its quantitative absorption-based imaging in true 3D that allows objects of interest to be viewed and measured from any orientation. However, x-ray CT has not been useful at the level of single cells because there is insufficient contrast to form an image. Recently, optical CT has been developed successfully for fixed cells, but this technology called Cell-CT is incompatible with live-cell imaging due to the use of stains, such as hematoxylin, that are not compatible with cell viability. We present a novel development of optical CT for quantitative, multispectral functional 4D (three spatial + one spectral dimension) imaging of living single cells. The method applied to immune system cells offers truly isotropic 3D spatial resolution and enables time-resolved imaging studies of cells suspended in aqueous medium. Using live-cell optical CT, we found a heterogeneous response to mitochondrial fission inhibition in mouse macrophages and differential basal remodeling of small (0.1 to 1 fl) and large (1 to 20 fl) nuclear and mitochondrial structures on a 20- to 30-s time scale in human myelogenous leukemia cells. Because of its robust 3D measurement capabilities, live-cell optical CT represents a powerful new tool in the biomedical research field.

  13. Home and the social worlds beyond: exploring influences in the lives of children of mothers with intellectual disability.

    Science.gov (United States)

    Collings, S; Llewellyn, G; Grace, R

    2017-09-01

    Mothers with intellectual disability are likely to raise their children in socially disadvantaged circumstances, and many face social isolation; however, the impact of a potentially restricted home context on children's social worlds has not been examined. This study was conducted to explore influences in the social worlds of children of mothers with intellectual disability from a child's perspective. Seven children aged 7 to 11 years took part in at least two semi-structured interviews over a year. Narrative accounts of each child's social interactions were analysed to ascertain if a pattern was present across the group. Home was found to influence the children's social interactions elsewhere by providing (or not) predictable routines and rules, and support from a significant adult other than a mother. Home environments were found to influence other social worlds by establishing a foundation for children's expectations about social interactions. The social worlds of school-aged children of mothers with intellectual disability are shaped by influences in the home that cannot be attributed exclusively to having a parent with intellectual disability. Significant adults provide an important support role and can be fulfilled by social service workers when a family-centred approach is applied. © 2017 John Wiley & Sons Ltd.

  14. Cost and impact of scaling up interventions to save lives of mothers and children: taking South Africa closer to MDGs 4 and 5

    Directory of Open Access Journals (Sweden)

    Lumbwe Chola

    2015-04-01

    Full Text Available Background: South Africa has made substantial progress on child and maternal mortality, yet many avoidable deaths of mothers and children still occur. This analysis identifies priority interventions to be scaled up nationally and projects the potential maternal and child lives saved. Design: We modelled the impact of maternal, newborn and child interventions using the Lives Saved Tools Projections to 2015 and used realistic coverage increases based on expert opinion considering recent policy change, financial and resource inputs, and observed coverage change. A scenario analysis was undertaken to test the impact of increasing intervention coverage to 95%. Results: By 2015, with realistic coverage, the maternal mortality ratio (MMR can reduce to 153 deaths per 100,000 and child mortality to 34 deaths per 1,000 live births. Fifteen interventions, including labour and delivery management, early HIV treatment in pregnancy, prevention of mother-to-child transmission and handwashing with soap, will save an additional 9,000 newborns and children and 1,000 mothers annually. An additional US$370 million (US$7 per capita will be required annually to scale up these interventions. When intervention coverage is increased to 95%, breastfeeding promotion becomes the top intervention, the MMR reduces to 116 and the child mortality ratio to 23. Conclusions: The 15 interventions identified were adopted by the National Department of Health, and the Health Minister launched a campaign to encourage Provincial Health Departments to scale up coverage. It is hoped that by focusing on implementing these 15 interventions at high quality, South Africa will reach Millennium Development Goal (MDG 4 soon after 2015 and MDG 5 several years later. Focus on HIV and TB during early antenatal care is essential. Strategic gains could be realised by targeting vulnerable populations and districts with the worst health outcomes. The analysis demonstrates the usefulness of priority

  15. Single-molecule live-cell imaging of bacterial DNA repair and damage tolerance.

    Science.gov (United States)

    Ghodke, Harshad; Ho, Han; van Oijen, Antoine M

    2018-02-19

    Genomic DNA is constantly under threat from intracellular and environmental factors that damage its chemical structure. Uncorrected DNA damage may impede cellular propagation or even result in cell death, making it critical to restore genomic integrity. Decades of research have revealed a wide range of mechanisms through which repair factors recognize damage and co-ordinate repair processes. In recent years, single-molecule live-cell imaging methods have further enriched our understanding of how repair factors operate in the crowded intracellular environment. The ability to follow individual biochemical events, as they occur in live cells, makes single-molecule techniques tremendously powerful to uncover the spatial organization and temporal regulation of repair factors during DNA-repair reactions. In this review, we will cover practical aspects of single-molecule live-cell imaging and highlight recent advances accomplished by the application of these experimental approaches to the study of DNA-repair processes in prokaryotes. © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.

  16. Quantitative control of mitochondria transfer between live single cells using a microfluidic device

    Directory of Open Access Journals (Sweden)

    Ken-Ichi Wada

    2017-12-01

    Full Text Available Quantitative control of mitochondria transfer between live cells is a promising approach for genetic manipulation of mitochondrial DNA (mtDNA because single mitochondrion transfer to a mtDNA-less (ρ0 cell potentially leads to homoplasmy of mtDNA. In this paper, we describe a method for quantitative control of mitochondria transfer between live single cells. For this purpose, we fabricated novel microfluidic devices having cell paring structures with a 4.1, 5.6 or 10.0 μm-length microtunnel. When cells were fused through a microtunnel using the Sendai virus envelope-based method, a strictured cytoplasmic connection was achieved with a length corresponding to that of the microtunnel. Elongation of the cytoplasmic connection led to a decrease in mitochondria transfer to the fusion partner. Moreover, some cell pairs that fused through a 10.0 μm-length microtunnel showed single mitochondrion transfer. Fused cells were spontaneously disconnected from each other when they were recovered in a normal culture medium. These results suggest that our cell fusion method can perform quantitative control of mitochondria transfer that includes a single mitochondrion transfer.

  17. Microscale consolidation analysis of relaxation behavior of single living chondrocytes subjected to varying strain-rates.

    Science.gov (United States)

    Nguyen, Trung Dung; Oloyede, Adekunle; Singh, Sanjleena; Gu, YuanTong

    2015-09-01

    Besides the elastic stiffness, the relaxation behavior of single living cells is also of interest of various researchers when studying cell mechanics. It is hypothesized that the relaxation response of the cells is governed by both intrinsic viscoelasticity of the solid phase and fluid-solid interactions mechanisms. There are a number of mechanical models have been developed to investigate the relaxation behavior of single cells. However, there is lack of model enable to accurately capture both of the mechanisms. Therefore, in this study, the porohyperelastic (PHE) model, which is an extension of the consolidation theory, combined with inverse Finite Element Analysis (FEA) technique was used at the first time to investigate the relaxation response of living chondrocytes. This model was also utilized to study the dependence of relaxation behavior of the cells on strain-rates. The stress-relaxation experiments under the various strain-rates were conducted with the Atomic Force Microscopy (AFM). The results have demonstrated that the PHE model could effectively capture the stress-relaxation behavior of the living chondrocytes, especially at intermediate to high strain-rates. Although this model gave some errors at lower strain-rates, its performance was acceptable. Therefore, the PHE model is properly a promising model for single cell mechanics studies. Moreover, it has been found that the hydraulic permeability of living chondrocytes reduced with decreasing of strain-rates. It might be due to the intracellular fluid volume fraction and the fluid pore pressure gradients of chondrocytes were higher when higher strain-rates applied. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. The mother relationship and artistic inhibition in the lives of Leonardo da Vinci and Erik H. Erikson.

    Science.gov (United States)

    Capps, Donald

    2008-12-01

    In four earlier articles, I focused on the theme of the relationship of melancholia and the mother, and suggested that the melancholic self may experience humor (Capps, 2007a), play (Capps, 2007b), dreams (Capps, 2008a), and art (Capps, 2008b) as restorative resources. I argued that Erik H. Erikson found these resources to be valuable remedies for his own melancholic condition, which had its origins in the fact that he was illegitimate and was raised solely by his mother until he was three years old, when she remarried. In this article, I focus on two themes in Freud's Leonardo da Vinci and a memory of his childhood (1964): Leonardo's relationship with his mother in early childhood and his inhibitions as an artist. I relate these two themes to Erikson's own early childhood and his failure to achieve his goal as an aspiring artist in his early twenties. The article concludes with a discussion of Erikson's frustrated aspirations to become an artist and his emphasis, in his psychoanalytic work, on children's play.

  19. Insecure attachment style as a vulnerability factor for depression: recent findings in a community-based study of Malay single and married mothers.

    Science.gov (United States)

    Abdul Kadir, Nor Ba'yah; Bifulco, Antonia

    2013-12-30

    The role of marital breakdown in women's mental health is of key concern in Malaysia and internationally. A cross-sectional questionnaire study of married and separated/divorced and widowed women examined insecure attachment style as an associated risk factor for depression among 1002 mothers in an urban community in Malaysia. A previous report replicated a UK-based vulnerability-provoking agent model of depression involving negative evaluation of self (NES) and negative elements in close relationships (NECRs) interacting with severe life events to model depression. This article reports on the additional contribution of insecure attachment style to the model using the Vulnerable Attachment Style Questionnaire (VASQ). The results showed that VASQ scores were highly correlated with NES, NECR and depression. A multiple regression analysis of depression with backward elimination found that VASQ scores had a significant additional effect. Group comparisons showed different risk patterns for single and married mothers. NES was the strongest risk factor for both groups, with the 'anxious style' subset of the VASQ being the best additional predictor for married mothers and the total VASQ score (general attachment insecurity) for single mothers. The findings indicate that attachment insecurity adds to a psychosocial vulnerability model of depression among mothers cross-culturally and is important in understanding and identifying risk. © 2013 Elsevier Ireland Ltd. All rights reserved.

  20. Association between ABO blood type and live-birth outcomes in single-embryo transfer cycles.

    Science.gov (United States)

    Pereira, Nigel; Patel, Hency H; Stone, Logan D; Christos, Paul J; Elias, Rony T; Spandorfer, Steven D; Rosenwaks, Zev

    2017-11-01

    To investigate the association between ABO blood type and live-birth outcomes in patients undergoing IVF with day 5 single-embryo transfer (SET). Retrospective cohort study. University-affiliated center. Normal responders, blood type and live birth, while controlling for confounders. Odds ratios (OR) with 95% confidence intervals (CI) for live birth were estimated. A total of 2,329 patients were included. The mean age of the study cohort was 34.6 ± 4.78 years. The distribution of blood types was as follows: A = 897 (38.5%); B = 397 (17.0%); AB = 120 (5.2%); and, O = 1,915 (39.3%) patients. There was no difference in the baseline demographics, ovarian stimulation, or embryo quality parameters between the blood types. The unadjusted ORs for live birth when comparing blood type A (referent) with blood types B, AB, and O were 0.96 (95% CI, 0.6-1.7), 0.72 (95% CI, 0.4-1.2), and 0.96 (95% CI. 0.6-1.7), respectively. The adjusted ORs for live birth remained not significant when comparing blood type A to blood types B, AB, and O individually. No difference in birth weight or gestational age at delivery was noted among the four blood types. Our findings suggest that ABO blood type is not associated with live-birth rate, birth weight, or gestational age at delivery in patients undergoing IVF with day 5 SET. Copyright © 2017 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  1. Life situation and identity among single older home-living people: A phenomenological–hermeneutic study

    Directory of Open Access Journals (Sweden)

    Bjørg Dale

    2012-07-01

    Full Text Available Being able to continue living in their own home as long as possible is the general preference for many older people, and this is also in line with the public policy in the Nordic countries. The aim of this study was to elucidate the meaning of self-care and health for perception of life situation and identity among single-living older individuals in rural areas in southern Norway. Eleven older persons with a mean age of 78 years were interviewed and encouraged to narrate their self-care and health experiences. The interviews were audio taped, transcribed verbatim and analysed using a phenomenological–hermeneutic method inspired by the philosophy of Ricoeur. The findings are presented as a naïve reading, an inductive structural analysis characterized by two main themes; i.e., “being able to do” and “being able to be”, and a comprehensive interpretation. The life situation of the interviewed single-living older individuals in rural areas in southern Norway was interpreted as inevitable, appropriate and meaningful. Their identity was constituted by their freedom and self-chosen actions in their personal contexts. The overall impression was that independence and the ability to control and govern their own life in accordance with needs and preferences were ultimate goals for the study participants.

  2. Life situation and identity among single older home-living people: a phenomenological-hermeneutic study.

    Science.gov (United States)

    Dale, Bjørg; Söderhamn, Ulrika; Söderhamn, Olle

    2012-01-01

    Being able to continue living in their own home as long as possible is the general preference for many older people, and this is also in line with the public policy in the Nordic countries. The aim of this study was to elucidate the meaning of self-care and health for perception of life situation and identity among single-living older individuals in rural areas in southern Norway. Eleven older persons with a mean age of 78 years were interviewed and encouraged to narrate their self-care and health experiences. The interviews were audio taped, transcribed verbatim and analysed using a phenomenological-hermeneutic method inspired by the philosophy of Ricoeur. The findings are presented as a naïve reading, an inductive structural analysis characterized by two main themes; i.e., "being able to do" and "being able to be", and a comprehensive interpretation. The life situation of the interviewed single-living older individuals in rural areas in southern Norway was interpreted as inevitable, appropriate and meaningful. Their identity was constituted by their freedom and self-chosen actions in their personal contexts. The overall impression was that independence and the ability to control and govern their own life in accordance with needs and preferences were ultimate goals for the study participants.

  3. Life situation and identity among single older home-living people: A phenomenological–hermeneutic study

    Science.gov (United States)

    Söderhamn, Ulrika; Söderhamn, Olle

    2012-01-01

    Being able to continue living in their own home as long as possible is the general preference for many older people, and this is also in line with the public policy in the Nordic countries. The aim of this study was to elucidate the meaning of self-care and health for perception of life situation and identity among single-living older individuals in rural areas in southern Norway. Eleven older persons with a mean age of 78 years were interviewed and encouraged to narrate their self-care and health experiences. The interviews were audio taped, transcribed verbatim and analysed using a phenomenological–hermeneutic method inspired by the philosophy of Ricoeur. The findings are presented as a naïve reading, an inductive structural analysis characterized by two main themes; i.e., “being able to do” and “being able to be”, and a comprehensive interpretation. The life situation of the interviewed single-living older individuals in rural areas in southern Norway was interpreted as inevitable, appropriate and meaningful. Their identity was constituted by their freedom and self-chosen actions in their personal contexts. The overall impression was that independence and the ability to control and govern their own life in accordance with needs and preferences were ultimate goals for the study participants. PMID:22848230

  4. Learning Curve for Laparoendoscopic Single-Incision Live Donor Nephrectomy: Implications for Laparoendoscopic Practice and Training.

    Science.gov (United States)

    Troppmann, Christoph; Santhanakrishnan, Chandrasekar; Fananapazir, Ghaneh; Troppmann, Kathrin M; Perez, Richard V

    2017-05-01

    The learning curve for laparoendoscopic single-incision live donor nephrectomy, which is technically more complex than the multiport, conventional laparoendoscopic approach, is unknown. In a retrospective cohort study, we analyzed the learning curve of the initial 114 consecutive single-incision laparoendoscopic nephrectomies performed in nonselected live kidney donors. Median donor body mass index was 26 kg/m 2 (range 20-34). In all, 92% of the nephrectomies were performed on the left side; 18% of the recovered kidneys had multiple renal arteries. Cumulative sum (CUSUM) analysis of operating time (OT) demonstrated that the learning curve was achieved after case 61. For the learning curve phase (Group 1 [cases 1-61]) vs the postlearning phase (Group 2 [cases 62-114]), the difference of the mean OT was 20 minutes (p = 0.05). Mean warm ischemic time in the donors was significantly longer during the learning phase (Group 1, 6 minutes; Group 2, 5 minutes; p = 0.04). Rates of conversions to multiport procedures and of donor complications were not significantly different between Groups 1 and 2. For the recipients, we observed delayed graft function in 2 (2%) cases, no technical graft losses; and 1-year death-censored graft survival was 100% (p = n.s. for all comparisons of Group 1 vs 2). Single-incision laparoendoscopic donor nephrectomy had a long learning curve (>60 cases), but resulted in excellent donor and recipient outcomes. The long learning curve has significant implications for the programs and surgeons who contemplate transitioning from multiport to single-incision nephrectomy. Furthermore, our observations are highly relevant for informing the development of training requirements for fellows to be trained in single-incision laparoendoscopic nephrectomy.

  5. Hemicellulose-based multifunctional macroinitiator for single-electron-transfer mediated living radical polymerization.

    Science.gov (United States)

    Voepel, Jens; Edlund, Ulrica; Albertsson, Ann-Christine; Percec, Virgil

    2011-01-10

    A multifunctional macroinitiator for single-electron-transfer mediated living radical polymerization (SET-LRP) was designed from acetylated galactoglucomannan (AcGGM) by α-bromoisobutyric acid functionalization of the anomeric hydroxyl groups on the heteropolysaccharide backbone. This macroinitiator, with a degree of substitution of 0.15, was used in the SET-LRP of methyl acrylate, catalyzed by Cu(0)/Me(6)-TREN in DMSO, DMF, or DMSO/H(2)O in various concentrations. Kinetic analyses confirm high conversions of up to 99.98% and a living behavior of the SET-LRP process providing high molecular weight hemicelluloses/methyl acrylate hybrid copolymers with a brush-like architecture.

  6. Single Stance Stability and Proprioceptive Control in Older Adults Living at Home: Gender and Age Differences

    Directory of Open Access Journals (Sweden)

    Dario Riva

    2013-01-01

    Full Text Available In developed countries, falls in older people represent a rising problem. As effective prevention should start before the risk becomes evident, an early predictor is needed. Single stance instability would appear as a major risk factor. Aims of the study were to describe single stance stability, its sensory components, and their correlation with age and gender. A random sample of 597 older adults (319 men, 278 women living at home, aged 65–84, was studied. Stability tests were performed with an electronic postural station. The single stance test showed the impairment of single stance stability in older individuals (75–84 yrs. The significant decline of stability in the older subjects may be explained by the impairment of proprioceptive control together with the decrease in compensatory visual stabilization and emergency responses. Younger subjects (65–74 yrs exhibited better, but still inadequate, proprioceptive control with compensatory visual stabilization. Gender differences appeared in older subjects: women were significantly less stable than men. The measurement of the sensory components of single stance stability could aid in the early detection of a decay in antigravity movements many years before the risk of falling becomes evident. Adequate proprioceptive control could mitigate the effects of all other risks of falling.

  7. Simultaneous live cell imaging using dual FRET sensors with a single excitation light.

    Directory of Open Access Journals (Sweden)

    Yusuke Niino

    Full Text Available Fluorescence resonance energy transfer (FRET between fluorescent proteins is a powerful tool for visualization of signal transduction in living cells, and recently, some strategies for imaging of dual FRET pairs in a single cell have been reported. However, these necessitate alteration of excitation light between two different wavelengths to avoid the spectral overlap, resulting in sequential detection with a lag time. Thus, to follow fast signal dynamics or signal changes in highly motile cells, a single-excitation dual-FRET method should be required. Here we reported this by using four-color imaging with a single excitation light and subsequent linear unmixing to distinguish fluorescent proteins. We constructed new FRET sensors with Sapphire/RFP to combine with CFP/YFP, and accomplished simultaneous imaging of cAMP and cGMP in single cells. We confirmed that signal amplitude of our dual FRET measurement is comparable to of conventional single FRET measurement. Finally, we demonstrated to monitor both intracellular Ca(2+ and cAMP in highly motile cardiac myocytes. To cancel out artifacts caused by the movement of the cell, this method expands the applicability of the combined use of dual FRET sensors for cell samples with high motility.

  8. Longterm renal allograft survival after sequential liver-kidney transplantation from a single living donor.

    Science.gov (United States)

    Kitajima, Kumiko; Ogawa, Yuichi; Miki, Katsuyuki; Kai, Kotaro; Sannomiya, Akihito; Iwadoh, Kazuhiro; Murakami, Toru; Koyama, Ichiro; Nakajima, Ichiro; Fuchinoue, Shohei

    2017-03-01

    Combined liver-kidney transplantation (CLKT) is well established as a definitive therapy with the potential to provide complete recovery for certain liver-kidney diseases, although the results might be contingent on the cause of transplantation. The purposes of the present study were to review the longterm outcome of renal allografts in CLKT patients from single living donors and to investigate the beneficial factors, compared with solitary renal transplantation. Thirteen patients underwent sequential liver transplantation (LT) and kidney transplantation (KT) from single living donors. The indications for KT were oxaluria (n = 7), autosomal recessive polycystic disease (n = 3), and others (n = 3). The same immunosuppressive regimen used after LT was also used after KT. KT was performed between 1.7 and 47.0 months after the LT. The overall patient survival rate was 92.3% at 10 years. In 12 of the 13 surviving patients, the renal allografts were found to be functioning in 11 patients after a mean follow-up period of 103.6 months. The death-censored renal allograft survival rate at 10 years was 100%, which was better than that of KT alone (84.9%) in Japan. Immunological protection conferred by the preceding liver allograft may have contributed to the longterm outcomes of the renal allografts. In addition, the donation of double organs from a single living and related donor may have a favorable impact on the graft survival rate. In the future, investigations of factors affecting the longterm outcome of renal allografts, including details of the involvement of de novo donor-specific antibody, will be needed. Liver Transplantation 23 315-323 2017 AASLD. © 2016 by the American Association for the Study of Liver Diseases.

  9. Self-Esteem in Hispanic Adolescent Females and Its Relation to Dual Parent Households and Single Mother Households

    Science.gov (United States)

    Stevens, Linda Marie Pettis

    2002-01-01

    The development of a sense of self in adolescence has been shown to be influenced by the perceived level of warmth of the mother. Additionally, the nature of the home environment has been found to relate to an adolescent's level of self-esteem (Buri, 1990; Field, Lang, Yando, and Bendell, 1993). Hispanic adolescent females and their mothers in…

  10. Influence of group cohesion on maternal well-being among participants in a support/education group program for single mothers.

    Science.gov (United States)

    Lipman, Ellen L; Waymouth, Marjorie; Gammon, Tara; Carter, Patricia; Secord, Margaret; Leung, Olivia; Mills, Brenda; Hicks, Frances

    2007-10-01

    Single mothers are at increased risk of psychosocial disadvantage, social isolation and physical and mental health difficulties. The authors present (1) the results of group cohesion assessments completed by mothers participating in a trial of community-based support/education groups, and (2) assessments of the association between group cohesion ratings and intervention outcomes of maternal self-evaluations of well-being (mood, self-esteem, and social support) and parenting. Mothers participating in groups completed the Group Atmosphere Scale, a measure of group cohesion, post-group. Overall, most participants provided strong ratings of group cohesion. Significant associations were found between group cohesion and specific positive outcomes. This suggests a positive association between group cohesion and mood, self-esteem, social support, and parenting, in this trial.

  11. "On our own, we can't manage": experiences with infant feeding recommendations among Malawian mothers living with HIV

    Directory of Open Access Journals (Sweden)

    Sellen Daniel W

    2010-10-01

    Full Text Available Abstract Background Infant feeding in communities with a high prevalence of HIV and AIDS is a potential challenge for mothers who must ultimately decide how to feed their infants within contexts that constrain their choices. Methods We investigated how infant feeding policy recommendations translate into maternal infant feeding decisions and practices using ethnographic research conducted between August 2004 and June 2005 among women participating in a prevention of mother-to-child transmission (PMTCT program in Lilongwe, Malawi. Results Qualitative findings are that maternal ability to adhere to recommendations to breastfeed exclusively for the first six months of infant life was constrained by expectations and psycho-social support. The most salient were women's pre-existing views on breastfeeding, their understanding of the medico-scientific information, and the quality of counselling received. In contrast, maternal decisions to wean were largely influenced by household economic factors and food insecurity. Conclusions We conclude that PMTCT programs delivered in ways which "download" the responsibility of adhering to recommendations to women in the absence of adequate psycho-social and livelihood supports contribute to substantial maternal psychosocial distress in this and, likely, similar settings.

  12. Vibrio cholerae biofilm growth program and architecture revealed by single-cell live imaging.

    Science.gov (United States)

    Yan, Jing; Sharo, Andrew G; Stone, Howard A; Wingreen, Ned S; Bassler, Bonnie L

    2016-09-06

    Biofilms are surface-associated bacterial communities that are crucial in nature and during infection. Despite extensive work to identify biofilm components and to discover how they are regulated, little is known about biofilm structure at the level of individual cells. Here, we use state-of-the-art microscopy techniques to enable live single-cell resolution imaging of a Vibrio cholerae biofilm as it develops from one single founder cell to a mature biofilm of 10,000 cells, and to discover the forces underpinning the architectural evolution. Mutagenesis, matrix labeling, and simulations demonstrate that surface adhesion-mediated compression causes V. cholerae biofilms to transition from a 2D branched morphology to a dense, ordered 3D cluster. We discover that directional proliferation of rod-shaped bacteria plays a dominant role in shaping the biofilm architecture in V. cholerae biofilms, and this growth pattern is controlled by a single gene, rbmA Competition analyses reveal that the dense growth mode has the advantage of providing the biofilm with superior mechanical properties. Our single-cell technology can broadly link genes to biofilm fine structure and provides a route to assessing cell-to-cell heterogeneity in response to external stimuli.

  13. Synchrotron radiation infrared microspectroscopy of single living cells in microfluidic devices: advantages, disadvantages and future perspectives

    International Nuclear Information System (INIS)

    Vaccari, L; Birarda, G; Grenci, G; Pacor, S; Businaro, L

    2012-01-01

    The possibility to fully exploit the diagnostic capabilities of SR-IRMS for studying single living cells under physiological conditions is limited by several constrains. First of all, the technology for manufacturing materials transparent to both IR and visible light is quite immature, limiting the design of fluidic devices to simple demountable liquid cells. In addition, the water spectral features become prominent in the Mid IR, hiding several cellular bands and therefore limiting the diagnostic capabilities of SR-IRMS. The overcoming of the so called 'water absorption barrier' requires the improvement of the protocols for the compensation of buffer spectral contributions, a goal that can be achieved also advancing the quality of IR-suitable fluidic devices. In this paper, the technical solutions employed for microfabricating completely sealed IR-visible transparent fluidic devices for living cell analysis will be presented. Several examples of the results obtained in the study of living U937 monocytes subjected to different stimuli will be selected for highlighting both the advantages and the disadvantages offered by our approach for cellular biology.

  14. Successful comeback of the single-dose live oral cholera vaccine CVD 103-HgR.

    Science.gov (United States)

    Herzog, Christian

    2016-01-01

    Effective and easy to administer cholera vaccines are in need more than ever, for at risk populations and travellers alike. In many parts of the world cholera is still endemic, causing outbreaks and constituting repeatedly serious public health problems. The oral live cholera vaccine CVD 103-HgR (Orochol, Mutachol), the first genetically modified organism (GMO) used as vaccine, was in its time (launched 1993, Switzerland) the ideal cholera vaccine: single-dose, protective efficacy of 80-100% against moderate to severe cholera, acting within 8 days and exhibiting excellent safety, indiscernible from placebo. However, there were strong headwinds: In the 1990s the indication for cholera vaccines was generally downplayed by experts and in 1997 the European Commission called for a moratorium of GMOs which blocked the registration in the European Union. Thus, demand for this vaccine remained low and in 2003 it was taken off the market for economic reasons. After a decade in obscurity it (Vaxchora) has resurfaced again, now produced in the U.S. and equipped with a U.S. FDA license (June 10, 2016). What had happened? This commentary gives a critical account of an almost unbelievable string of misadventures, emerging adverse circumstances and man-made failures which nearly killed this single-dose live oral cholera vaccine. The good news is that patience and persistence lead to success in the end, allowing good science to prevail for the benefit of those in need. Copyright © 2016 Elsevier Ltd. All rights reserved.

  15. Vascular complications following 1500 consecutive living and cadaveric donor renal transplantations: A single center study

    International Nuclear Information System (INIS)

    Salehipour, Mehdi; Salahi, Heshmatollah; Jalaeian, Hamed; Bahador, Ali; Nikeghbalian, Saman; Barzideh, Ehsan; Ariafar, Ali; Malek-Hosseini, Seyed Ali

    2009-01-01

    The aim of this study was to document vascular complications that occurred following cadaveric and living donor kidney transplants in order to assess the overall incidence of these complications at our center as well as to identify possible risk factors. In a retrospective cohort study, 1500 consecutive renal transplant recipients who received a living or cadaveric donor kidney between December 1988 and July 2006 were evaluated. The study was performed at the Nemazee Hospital, Shiraz, Iran. The assessment of the anatomy and number of renal arteries as well as the incidence of vascular complications was made by color doppler ultrasonography, angiography, and/or surgical exploration. Clinically apparent vascular complications were seen in 8.86% of all study patients (n = 133) with the most frequent being hemorrhage (n = 91; 6.1%) followed by allograft renal artery stenosis (n = 26; 1.7%), renal artery thrombosis (n = 9; 0.6%), and renal vein thrombosis (n = 7; 0.5%). Vascular complications were more frequent in recipients of cadaveric organs than recipients of allografts from living donors (12.5% vs. 7.97%; P0.017). The occurrence of vascular complications was significantly more frequent among recipients of renal allografts with multiple arteries when compared with recipients of kidneys with single artery (12.3% vs. 8.2%; P0.033). The same was true to venous complications as well (25.4% vs. 8.2%; P< 0.001). Our study shows that vascular complications were more frequent in allografts with multiple renal blood vessels. Also, the complications were much less frequent in recipients of living donor transplants. (author)

  16. Diffusion dynamics of the Keap1–Cullin3 interaction in single live cells

    Energy Technology Data Exchange (ETDEWEB)

    Baird, Liam [Jacqui Wood Cancer Centre, Division of Cancer Research, Medical Research Institute, University of Dundee, Dundee, Scotland (United Kingdom); Dinkova-Kostova, Albena T., E-mail: a.dinkovakostova@dundee.ac.uk [Jacqui Wood Cancer Centre, Division of Cancer Research, Medical Research Institute, University of Dundee, Dundee, Scotland (United Kingdom); Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD (United States)

    2013-03-29

    Highlights: ► We developed a quantitative FRAP-based system to study the Keap1–Cul3 interaction. ► We show that Keap1–EGFP and mCherry–Cul3 interact in single live cells. ► We used inducers which target distinct cysteine sensors of Keap1 and differ 4000-fold in potency. ► Inducers cause Nrf2 stabilization, nuclear translocation, and target gene expression. ► Inducers of four different types do not dissociate the Keap1–EGFP:mCherry–Cul3 complex. -- Abstract: Transcription factor NF-E2 p45-related factor 2 (Nrf2) regulates the expression of a network of genes encoding drug-detoxification, anti-inflammatory, and metabolic enzymes, as well as proteins involved in the regulation of cellular redox homeostasis. Under basal conditions, Kelch-like ECH associated protein 1 (Keap1) targets Nrf2 for ubiquitination and proteasomal degradation via association with Cullin3 (Cul3)-based Rbx1 E3 ubiquitin ligase. Various small molecules (inducers) activate Nrf2 leading to upregulation of cytoprotective gene expression. Inducers chemically modify specific cysteine residues of Keap1 which ultimately loses its ability to target Nrf2 for degradation. Dissociation of the Keap1–Cul3 complex by inducers is one possible mechanism, but evidence in single live cells is lacking. To investigate the diffusion dynamics of the Keap1–Cul3 interaction and the effect of inducers, we developed a quantitative fluorescence recovery after photobleaching (FRAP)-based system using Keap1–EGFP and mCherry–Cul3 fusion proteins. We show that Keap1–EGFP and mCherry–Cul3 interact in single live cells. Exposure for 1 h to small-molecule inducers of 4 different types, the oleanane triterpenoid CDDO, the isothiocyanate sulforaphane, the sulfoxythiocarbamate STCA, and the oxidant hydrogen peroxide which target distinct cysteine sensors within Keap1 with potencies which differ by nearly 4000-fold, does not dissociate the Keap1–Cul3 complex. As inducers cause conformational changes

  17. Live-Cell Visualization of Pre-mRNA Splicing with Single-Molecule Sensitivity

    Directory of Open Access Journals (Sweden)

    Robert M. Martin

    2013-09-01

    Full Text Available Removal of introns from pre-messenger RNAs (pre-mRNAs via splicing provides a versatile means of genetic regulation that is often disrupted in human diseases. To decipher how splicing occurs in real time, we directly examined with single-molecule sensitivity the kinetics of intron excision from pre-mRNA in the nucleus of living human cells. By using two different RNA labeling methods, MS2 and λN, we show that β-globin introns are transcribed and excised in 20–30 s. Furthermore, we show that replacing the weak polypyrimidine (Py tract in mouse immunoglobulin μ (IgM pre-mRNA by a U-rich Py decreases the intron lifetime, thus providing direct evidence that splice-site strength influences splicing kinetics. We also found that RNA polymerase II transcribes at elongation rates ranging between 3 and 6 kb min−1 and that transcription can be rate limiting for splicing. These results have important implications for a mechanistic understanding of cotranscriptional splicing regulation in the live-cell context.

  18. Live cell imaging combined with high-energy single-ion microbeam

    International Nuclear Information System (INIS)

    Guo, Na; Du, Guanghua; Liu, Wenjing; Wu, Ruqun; Wei, Junzhe; Guo, Jinlong; Chen, Hao

    2016-01-01

    DNA strand breaks can lead to cell carcinogenesis or cell death if not repaired rapidly and efficiently. An online live cell imaging system was established at the high energy microbeam facility at the Institute of Modern Physics to study early and fast cellular response to DNA damage after high linear energy transfer ion radiation. The HT1080 cells expressing XRCC1-RFP were irradiated with single high energy nickel ions, and time-lapse images of the irradiated cells were obtained online. The live cell imaging analysis shows that strand-break repair protein XRCC1 was recruited to the ion hit position within 20 s in the cells and formed bright foci in the cell nucleus. The fast recruitment of XRCC1 at the ion hits reached a maximum at about 200 s post-irradiation and then was followed by a slower release into the nucleoplasm. The measured dual-exponential kinetics of XRCC1 protein are consistent with the proposed consecutive reaction model, and the measurements obtained that the reaction rate constant of the XRCC1 recruitment to DNA strand break is 1.2 × 10 −3 s −1 and the reaction rate constant of the XRCC1 release from the break-XRCC1 complex is 1.2 × 10 −2 s −1 .

  19. Live cell imaging combined with high-energy single-ion microbeam

    Energy Technology Data Exchange (ETDEWEB)

    Guo, Na; Du, Guanghua, E-mail: gh-du@impcas.ac.cn; Liu, Wenjing; Wu, Ruqun; Wei, Junzhe [Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou (China); Guo, Jinlong [Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou (China); Northwest Normal University, Lanzhou (China); Chen, Hao [Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou (China); Institute of Nuclear Science and Technology, University of Lanzhou, Lanzhou (China)

    2016-03-15

    DNA strand breaks can lead to cell carcinogenesis or cell death if not repaired rapidly and efficiently. An online live cell imaging system was established at the high energy microbeam facility at the Institute of Modern Physics to study early and fast cellular response to DNA damage after high linear energy transfer ion radiation. The HT1080 cells expressing XRCC1-RFP were irradiated with single high energy nickel ions, and time-lapse images of the irradiated cells were obtained online. The live cell imaging analysis shows that strand-break repair protein XRCC1 was recruited to the ion hit position within 20 s in the cells and formed bright foci in the cell nucleus. The fast recruitment of XRCC1 at the ion hits reached a maximum at about 200 s post-irradiation and then was followed by a slower release into the nucleoplasm. The measured dual-exponential kinetics of XRCC1 protein are consistent with the proposed consecutive reaction model, and the measurements obtained that the reaction rate constant of the XRCC1 recruitment to DNA strand break is 1.2 × 10{sup −3} s{sup −1} and the reaction rate constant of the XRCC1 release from the break-XRCC1 complex is 1.2 × 10{sup −2} s{sup −1}.

  20. Live cell imaging combined with high-energy single-ion microbeam

    Science.gov (United States)

    Guo, Na; Du, Guanghua; Liu, Wenjing; Guo, Jinlong; Wu, Ruqun; Chen, Hao; Wei, Junzhe

    2016-03-01

    DNA strand breaks can lead to cell carcinogenesis or cell death if not repaired rapidly and efficiently. An online live cell imaging system was established at the high energy microbeam facility at the Institute of Modern Physics to study early and fast cellular response to DNA damage after high linear energy transfer ion radiation. The HT1080 cells expressing XRCC1-RFP were irradiated with single high energy nickel ions, and time-lapse images of the irradiated cells were obtained online. The live cell imaging analysis shows that strand-break repair protein XRCC1 was recruited to the ion hit position within 20 s in the cells and formed bright foci in the cell nucleus. The fast recruitment of XRCC1 at the ion hits reached a maximum at about 200 s post-irradiation and then was followed by a slower release into the nucleoplasm. The measured dual-exponential kinetics of XRCC1 protein are consistent with the proposed consecutive reaction model, and the measurements obtained that the reaction rate constant of the XRCC1 recruitment to DNA strand break is 1.2 × 10-3 s-1 and the reaction rate constant of the XRCC1 release from the break-XRCC1 complex is 1.2 × 10-2 s-1.

  1. Effectiveness of ACT-Based Parenting Training to Mothers on the Depression of Children with Cleft Lip and Palate: A Single Subject Study

    Directory of Open Access Journals (Sweden)

    محمد صالح فقیهی

    2017-06-01

    Full Text Available The purpose of this study was to determine the effectiveness of Parenting Training based on Acceptance and Commitment Therapy (ACT to mothers on the depression of children with cleft lip and palate. The research method was based on a single case and individual intervention study. The sample was constituted of 65 Isfahanian children with cleft lip and palate. Parenting skills based on ACT were taught to five mothers of children with cleft lip and palate who achieved the minimum score in screening. After three baseline sessions for each child, ACT parenting skills were taught to their mothers in 8 individual sessions companied with testing the child’s depression in every session. Three follow-up sessions after 15 days, 1 month and 3 months were set to evaluate children’s depression. The Kovacs Children’s Depression Inventory (CDI was used to test the children’s depression. The results were analyzed with visual analysis and descriptive statistics. This particular intervention was effective on depression. Based on the results of the present study, it can be concluded that ACT parenting training to mothers of children with cleft lips and palates was effective on reducing depression and that an on-time intervention can improve these children's depression.

  2. A bright single-cell resolution live imaging reporter of Notch signaling in the mouse.

    Science.gov (United States)

    Nowotschin, Sonja; Xenopoulos, Panagiotis; Schrode, Nadine; Hadjantonakis, Anna-Katerina

    2013-04-25

    Live imaging provides an essential methodology for understanding complex and dynamic cell behaviors and their underlying molecular mechanisms. Genetically-encoded reporter expressing mouse strains are an important tool for use in live imaging experiments. Such reporter strains can be engineered by placing cis-regulatory elements of interest to direct the expression of desired reporter genes. If these cis-regulatory elements are downstream targets, and thus activated as a consequence of signaling pathway activation, such reporters can provide read-outs of the signaling status of a cell. The Notch signaling pathway is an evolutionary conserved pathway operating in multiple developmental processes as well as being the basis for several congenital diseases. The transcription factor CBF1 is a central evolutionarily conserved component of the Notch signaling pathway. It binds the active form of the Notch receptor (NICD) and subsequently binds to cis-regulatory regions (CBF1 binding sites) in the promoters of Notch responsive genes. In this way, CBF1 binding sites represent a good target for the design of a Notch signaling reporter. To generate a single-cell resolution Notch signaling reporter, we used a CBF responsive element to direct the expression of a nuclear-localized fluorescent protein. To do this, we linked 4 copies of a consensus CBF1 binding site to the basal simian virus 40 (SV40) promoter, placed this cassette in front of a fluorescent protein fusion comprising human histone H2B linked to the yellow fluorescent protein (YFP) Venus, one of the brightest available YFPs. We used the CBF:H2B-Venus construct to generate both transgenic embryonic mouse stem (ES) cell lines and a strain of transgenic mice that would report Notch signaling activity. By using multiple CBF1 binding sites together with a subcellular-localized, genetically-encoded fluorescent protein, H2B-Venus, we have generated a transgenic strain of mice that faithfully recapitulates Notch signaling

  3. Single myelin fiber imaging in living rodents without labeling by deep optical coherence microscopy.

    Science.gov (United States)

    Ben Arous, Juliette; Binding, Jonas; Léger, Jean-François; Casado, Mariano; Topilko, Piotr; Gigan, Sylvain; Boccara, A Claude; Bourdieu, Laurent

    2011-11-01

    Myelin sheath disruption is responsible for multiple neuropathies in the central and peripheral nervous system. Myelin imaging has thus become an important diagnosis tool. However, in vivo imaging has been limited to either low-resolution techniques unable to resolve individual fibers or to low-penetration imaging of single fibers, which cannot provide quantitative information about large volumes of tissue, as required for diagnostic purposes. Here, we perform myelin imaging without labeling and at micron-scale resolution with >300-μm penetration depth on living rodents. This was achieved with a prototype [termed deep optical coherence microscopy (deep-OCM)] of a high-numerical aperture infrared full-field optical coherence microscope, which includes aberration correction for the compensation of refractive index mismatch and high-frame-rate interferometric measurements. We were able to measure the density of individual myelinated fibers in the rat cortex over a large volume of gray matter. In the peripheral nervous system, deep-OCM allows, after minor surgery, in situ imaging of single myelinated fibers over a large fraction of the sciatic nerve. This allows quantitative comparison of normal and Krox20 mutant mice, in which myelination in the peripheral nervous system is impaired. This opens promising perspectives for myelin chronic imaging in demyelinating diseases and for minimally invasive medical diagnosis.

  4. Opto-injection into single living cells by femtosecond near-infrared laser

    Science.gov (United States)

    Peng, Cheng

    This dissertation presents a novel technique to deliver membrane impermeable molecules into single living cells with the assistance of femtosecond (fs) near-infrared (NIR) laser pulses. This approach merges ultrafast laser technology with key biological, biomedical, and medical applications, such as gene transfection, gene therapy and drug delivery. This technique promises several major advantages, namely, very high transfection efficiency, high cell survival rate (≈100%) and fully preserved cell viabilities. It is also a promising method to deliver molecules into cells that are difficult or even completely resistant to established physical methods, such as microinjection by glass pipettes, electroporation, and biolistics. In this work, the system for fs NIR opto-injection was designed and built. Successful fs NIR opto-injection has been performed on several cell systems including single mammalian cells (bovine aortic endothelial cells), marine animal eggs (Spisula solidissima oocytes), and human cancer cells (fibrosarcoma HT1080) cultured in a tissue-like environment. The connections between laser parameters and cell responses were explored through further experiments and in-depth analyses, especially the relationship between dye uptake rate and incident laser intensity, and the relationship between pore size created on cell membranes and incident laser intensity. Dye uptake rate of the target cells was observed to depend on incident laser intensity. Pore size was found dependent on incident laser intensity. The conclusion was made that laser-induced breakdown and plasma-induced ablation in cell membrane are the physical principles that govern the process of fs NIR opto-injection.

  5. Mothering against the Odds: Diverse Voices of Contemporary Mothers.

    Science.gov (United States)

    Coll, Cynthia Garcia, Ed.; Surrey, Janet L., Ed.; Weingarten, Kathy, Ed.

    Based on the view that increasing numbers of mothers who do not fit a narrow traditional image are often maligned, misunderstood, or ignored, this book presents the stories of a diverse group of mothers whose life circumstances place them outside the mainstream. Chapters explore the lives of mothers of exceptional children and biracial children;…

  6. Developing new optical imaging techniques for single particle and molecule tracking in live cells

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Wei [Iowa State Univ., Ames, IA (United States)

    2010-01-01

    Differential interference contrast (DIC) microscopy is a far-field as well as wide-field optical imaging technique. Since it is non-invasive and requires no sample staining, DIC microscopy is suitable for tracking the motion of target molecules in live cells without interfering their functions. In addition, high numerical aperture objectives and condensers can be used in DIC microscopy. The depth of focus of DIC is shallow, which gives DIC much better optical sectioning ability than those of phase contrast and dark field microscopies. In this work, DIC was utilized to study dynamic biological processes including endocytosis and intracellular transport in live cells. The suitability of DIC microscopy for single particle tracking in live cells was first demonstrated by using DIC to monitor the entire endocytosis process of one mesoporous silica nanoparticle (MSN) into a live mammalian cell. By taking advantage of the optical sectioning ability of DIC, we recorded the depth profile of the MSN during the endocytosis process. The shape change around the nanoparticle due to the formation of a vesicle was also captured. DIC microscopy was further modified that the sample can be illuminated and imaged at two wavelengths simultaneously. By using the new technique, noble metal nanoparticles with different shapes and sizes were selectively imaged. Among all the examined metal nanoparticles, gold nanoparticles in rod shapes were found to be especially useful. Due to their anisotropic optical properties, gold nanorods showed as diffraction-limited spots with disproportionate bright and dark parts that are strongly dependent on their orientation in the 3D space. Gold nanorods were developed as orientation nanoprobes and were successfully used to report the self-rotation of gliding microtubules on kinesin coated substrates. Gold nanorods were further used to study the rotational motions of cargoes during the endocytosis and intracellular transport processes in live mammalian

  7. Single-living is associated with increased risk of long-term mortality among employed patients with acute myocardial infarction

    Directory of Open Access Journals (Sweden)

    Finn Erl

    2010-05-01

    Full Text Available Finn Erland Nielsen, Shan MardDepartment of Cardiology S, Herlev University Hospital, DenmarkObjective: There is conflicting evidence about the impact of social support on adverse outcome after acute myocardial infarction (MI. We examined the relation between single-living and long-term all-cause mortality after MI.Design: A prospective cohort study of 242 employed patients with MI followed up to 16 years after MI.Results: A total of 106 (43.8% patients died during the follow-up. Single-living nearly doubled the risk of death; after adjusting for potential confounding factors, single-living was an independent predictor of death, with a hazard ratio of 2.55 (95% confidence interval: 1.52–4.30. Other predictors of death were diabetes mellitus, atrial fibrillation, age, and ejection fraction less than 35%.Conclusion: Single-living is a prognostic determinant of long-term all-cause mortality after MI.Keywords: acute myocardial infarction, social support, single-living, prognosis.

  8. What’s Gay About Being Single? A Qualitative Study of the Lived Experiences of Older Single Gay Men

    OpenAIRE

    Yiu Tung Suen

    2015-01-01

    This paper contributes to the theorization of ‘choice’ within sociological understanding of singlehood. Previous sociological research on singlehood has largely focused on the lives of heterosexual singles. A choice narrative permeates such literature, depicting singlehood as a celebratory story that brings about the potential to disrupt the couplehood culture in society. Based on in-depth interviews with 25 self-identified single gay men over the age of 50 in England, this article finds ...

  9. Single-molecule motions and interactions in live cells reveal target search dynamics in mismatch repair.

    Science.gov (United States)

    Liao, Yi; Schroeder, Jeremy W; Gao, Burke; Simmons, Lyle A; Biteen, Julie S

    2015-12-15

    MutS is responsible for initiating the correction of DNA replication errors. To understand how MutS searches for and identifies rare base-pair mismatches, we characterized the dynamic movement of MutS and the replisome in real time using superresolution microscopy and single-molecule tracking in living cells. We report that MutS dynamics are heterogeneous in cells, with one MutS population exploring the nucleoid rapidly, while another MutS population moves to and transiently dwells at the replisome region, even in the absence of appreciable mismatch formation. Analysis of MutS motion shows that the speed of MutS is correlated with its separation distance from the replisome and that MutS motion slows when it enters the replisome region. We also show that mismatch detection increases MutS speed, supporting the model for MutS sliding clamp formation after mismatch recognition. Using variants of MutS and the replication processivity clamp to impair mismatch repair, we find that MutS dynamically moves to and from the replisome before mismatch binding to scan for errors. Furthermore, a block to DNA synthesis shows that MutS is only capable of binding mismatches near the replisome. It is well-established that MutS engages in an ATPase cycle, which is necessary for signaling downstream events. We show that a variant of MutS with a nucleotide binding defect is no longer capable of dynamic movement to and from the replisome, showing that proper nucleotide binding is critical for MutS to localize to the replisome in vivo. Our results provide mechanistic insight into the trafficking and movement of MutS in live cells as it searches for mismatches.

  10. The lifelong socioeconomic disadvantage of single-mother background - the Helsinki Birth Cohort study 1934–1944

    Directory of Open Access Journals (Sweden)

    H. Maiju Mikkonen

    2016-08-01

    Full Text Available Abstract Background Growing up with one parent is associated with economic hardship and health disadvantages, but there is limited evidence of its lifetime consequences. We examined whether being born to an unmarried mother is associated with socioeconomic position and marital history over the lifespan. Methods We analysed data from the Helsinki Birth Cohort Study including birth, child welfare clinic and school healthcare records from people born in Helsinki, Finland, between 1934 and 1944. Using a unique personal identification number, we linked these data to information on adult socioeconomic position from census data at 5-year intervals between 1970 and 2000, obtained from Statistics Finland. Results Compared to children of married mothers, children of unmarried mothers were more likely to have lower educational attainment and occupational status (odds ratio for basic vs. tertiary education 3.40; 95 % confidence interval 2.17 to 5.20; for lowest vs. highest occupational category 2.75; 1.92 to 3.95. They were also less likely to reach the highest income third in adulthood and more likely to stay unmarried themselves. The associations were also present when adjusted for childhood socioeconomic position. Conclusion Being born to an unmarried mother, in a society where marriage is the norm, is associated with socioeconomic disadvantage throughout life, over and above the disadvantage associated with childhood family occupational status. This disadvantage may in part mediate the association between low childhood socioeconomic position and health in later life.

  11. Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution.

    Science.gov (United States)

    B, Vinoth; Lai, Xin-Ji; Lin, Yu-Chih; Tu, Han-Yen; Cheng, Chau-Jern

    2018-04-13

    Digital holographic microtomography is a promising technique for three-dimensional (3D) measurement of the refractive index (RI) profiles of biological specimens. Measurement of the RI distribution of a free-floating single living cell with an isotropic superresolution had not previously been accomplished. To the best of our knowledge, this is the first study focusing on the development of an integrated dual-tomographic (IDT) imaging system for RI measurement of an unlabelled free-floating single living cell with an isotropic superresolution by combining the spatial frequencies of full-angle specimen rotation with those of beam rotation. A novel 'UFO' (unidentified flying object) like shaped coherent transfer function is obtained. The IDT imaging system does not require any complex image-processing algorithm for 3D reconstruction. The working principle was successfully demonstrated and a 3D RI profile of a single living cell, Candida rugosa, was obtained with an isotropic superresolution. This technology is expected to set a benchmark for free-floating single live sample measurements without labeling or any special sample preparations for the experiments.

  12. Convergence of lateral dynamic measurements in the plasma membrane of live cells from single particle tracking and STED-FCS

    DEFF Research Database (Denmark)

    Lagerholm, B. Christoffer; Andrade, Débora M.; Clausen, Mathias P.

    2017-01-01

    Fluorescence correlation spectroscopy (FCS) in combination with the super-resolution imaging method STED (STED-FCS), and single-particle tracking (SPT) are able to directly probe the lateral dynamics of lipids and proteins in the plasma membrane of live cells at spatial scales much below the diff......Fluorescence correlation spectroscopy (FCS) in combination with the super-resolution imaging method STED (STED-FCS), and single-particle tracking (SPT) are able to directly probe the lateral dynamics of lipids and proteins in the plasma membrane of live cells at spatial scales much below...... the diffraction limit of conventional microscopy. However, a major disparity in interpretation of data from SPT and STED-FCS remains, namely the proposed existence of a very fast (unhindered) lateral diffusion coefficient, ≥5 μm2 s-1, in the plasma membrane of live cells at very short length scales, ≈ 100 nm...

  13. Living Related Donor Kidney Transplantation in Libya: A Single Center Experience

    Directory of Open Access Journals (Sweden)

    Elusta Ahmed

    2008-01-01

    Full Text Available The aim of this study is to report the experience from a single center in Libya, on the prevailing live-related kidney transplantation program. The results of three years work on kidney transplantation at the Tripoli Central Hospital (National Organ Transplant Program in Libya were evaluated. The transplant program was launched on 17 th August, 2004 and 135 patients have been transplanted since then till 17 th August, 2007. All donors and recipients were screened thoroughly prior to transplant and monitored closely in the post-transplant period. Our immuno-suppressive protocol was cyclosporine-based. Among the 135 accepted pairs, donors and reci-pients were genetically-related in 133 cases (98.5% and emotionally-related in two others. The mean donor age was 37 ± 9.5 years (range 18-56 years and recipient age 37 ± 13.6 years (range 7-67 years. There were 95 males (70.4% and 40 females (29.6% among the recipients while among the donors, there were 102 males (75.6% and 33 females (24.4%. Delayed graft function was seen in three patients (2.2%, acute rejection in six (4.4%, post-transplant urinary tract infection in six (4.4%, pneumonia in three (2.2%, ureteric kink in two (1.5% and urine leak in four (3.0%. Graft survival at 36 months was 93.3% while patient survival at the same period was 96.3%. This report indicates that the results of our transplant program are good and comparable with other international programs.

  14. Measurement of separase proteolytic activity in single living cells by a fluorogenic flow cytometry assay.

    Directory of Open Access Journals (Sweden)

    Wiltrud Haaß

    Full Text Available ESPL1/Separase, an endopeptidase, is required for centrosome duplication and separation of sister-chromatides in anaphase of mitosis. Overexpression and deregulated proteolytic activity of Separase as frequently observed in human cancers is associated with the occurrence of supernumerary centrosomes, chromosomal missegregation and aneuploidy. Recently, we have hypothesized that increased Separase proteolytic activity in a small subpopulation of tumor cells may serve as driver of tumor heterogeneity and clonal evolution in chronic myeloid leukemia (CML. Currently, there is no quantitative assay to measure Separase activity levels in single cells. Therefore, we have designed a flow cytometry-based assay that utilizes a Cy5- and rhodamine 110 (Rh110-biconjugated Rad21 cleavage site peptide ([Cy5-D-R-E-I-M-R]2-Rh110 as smart probe and intracellular substrate for detection of Separase enzyme activity in living cells. As measured by Cy5 fluorescence the cellular uptake of the fluorogenic peptide was fast and reached saturation after 210 min of incubation in human histiocytic lymphoma U937 cells. Separase activity was recorded as the intensity of Rh110 fluorescence released after intracellular peptide cleavage providing a linear signal gain within a 90-180 min time slot. Compared to conventional cell extract-based methods the flow cytometric assay delivers equivalent results but is more reliable, bypasses the problem of vague loading controls and unspecific proteolysis associated with whole cell extracts. Especially suited for the investigaton of blood- and bone marrow-derived hematopoietic cells the flow cytometric Separase assay allows generation of Separase activity profiles that tell about the number of Separase positive cells within a sample i.e. cells that currently progress through mitosis and about the range of intercellular variation in Separase activity levels within a cell population. The assay was used to quantify Separase proteolytic

  15. Neither single, nor in a couple. A study of living apart together in France

    Directory of Open Access Journals (Sweden)

    Arnaud Regnier-Loilier

    2009-07-01

    Full Text Available Among living arrangements, living apart together relationships arouse curiosity on the part of sociologists, demographers and even the media. From a scientific point of view, how have noncohabiting relationships evolved in recent decades? How can we recognise these relationships, and who are the populations concerned? The present study provides an overall view into noncohabiting relationships in France, shedding light on the characteristics of both the individuals concerned and their relationships. There has been no recent increase in the prevalence of this living arrangement. It competes with cohabiting relationships both among students and among people with cohabiting children. Four main groups of living apart relationships are described: "Young adults", "Out of a family", "Seniors." The reasons for living apart as well as future intentions vary considerably across these groups.

  16. Single particle tracking-based reaction progress kinetic analysis reveals a series of molecular mechanisms of cetuximab-induced EGFR processes in a single living cell.

    Science.gov (United States)

    Kim, Do-Hyeon; Kim, Dong-Kyun; Zhou, Kai; Park, Soyeon; Kwon, Yonghoon; Jeong, Min Gyu; Lee, Nam Ki; Ryu, Sung Ho

    2017-07-01

    Cellular processes occur through the orchestration of multi-step molecular reactions. Reaction progress kinetic analysis (RPKA) can provide the mechanistic details to elucidate the multi-step molecular reactions. However, current tools have limited ability to simultaneously monitor dynamic variations in multiple complex states at the single molecule level to apply RPKA in living cells. In this research, a single particle tracking-based reaction progress kinetic analysis (sptRPKA) was developed to simultaneously determine the kinetics of multiple states of protein complexes in the membrane of a single living cell. The subpopulation ratios of different states were quantitatively (and statistically) reliably extracted from the diffusion coefficient distribution rapidly acquired by single particle tracking at constant and high density over a long period of time using super-resolution microscopy. Using sptRPKA, a series of molecular mechanisms of epidermal growth factor receptor (EGFR) cellular processing induced by cetuximab were investigated. By comprehensively measuring the rate constants and cooperativity of the molecular reactions involving four EGFR complex states, a previously unknown intermediate state was identified that represents the rate limiting step responsible for the selectivity of cetuximab-induced EGFR endocytosis to cancer cells.

  17. World kidney day 2010: medical aspects of 10 live-donor renal transplantations in a single center from a developing country.

    Science.gov (United States)

    Shah, P R; Modi, P R; Kute, V B; Vanikar, A V; Patel, H V; Gumber, M R; Shah, V R; Trivedi, H L

    2012-01-01

    World Kidney Day (WKD) has become the most widely celebrated event associated with kidney disease in the world and the most successful effort to raise awareness among both the general public and government health officials about the dangers of kidney disease. We celebrated WKD 2010 in a unique way by performing 10 live-donor renal transplantations (RTx) on March 11, 2010. We report a single-center experience on RTx vis-à-vis patient/graft survival, graft function in terms of serum creatinine (SCr) level, and rejection episodes in 10 live-donor RTx performed on WKD. Recipient diseases leading to end-stage renal disease (ESRD) were chronic glomerulonephritis (60%), benign nephrosclerosis (20%), and chronic interstitial nephritis (20%). Mean recipient age was 35 ± 8.7 years. Nine recipients were males. Mean donor age was 37 ± 8.7 years, Nine donors were females. Donors were spouse (n = 6), mother (n = 2), sister (n = 1), and extended family member (n = 1), with mean HLA match 1.8 ± 1.48. All patients received steroids, calcinueurin inhibitors, and mycophenolate mofetil/azathioprime for maintenance immunosuppression. During a mean follow-up time of 18 months, patient and graft survival rates were 90% and 90%, respectively, with a mean SCr level of 1.21 mg/dL; 20% had biopsy-proven acute rejection. One patient died due to infection after antirejection therapy. RTx has acceptable graft and patient survival. RTx is the best cost-effective therapeutic modality for patients suffering from ESRD and should be encouraged in view of organ shortage on events such as WKD. To our knowledge, this is the first report from a developing country on 10 successful RTx on WKD. Copyright © 2012 Elsevier Inc. All rights reserved.

  18. Biological aspects of the development and self-concept in adolescents living in single-parent families.

    OpenAIRE

    Veček, Andrea; Vidović, Vesna; Miličić, Jasna; Špoljar-Vržina, Sanja; Veček, Nenad; Arch-Veček, Branka

    2009-01-01

    In this study we investigate whether there are differences between adolescents who grow up in single-parent families and those who grow up in nucleus families. We have decided that there are no differences in the physical development between the adolescents who are growing up in single parent families and those growing up in nucleus families. There is no difference in the self-concept between these two groups, except in the ethical and moral self-image of adolescents living with one parent. A...

  19. Imaging Live Cells at the Nanometer-Scale with Single-Molecule Microscopy: Obstacles and Achievements in Experiment Optimization for Microbiology

    Science.gov (United States)

    Haas, Beth L.; Matson, Jyl S.; DiRita, Victor J.; Biteen, Julie S.

    2015-01-01

    Single-molecule fluorescence microscopy enables biological investigations inside living cells to achieve millisecond- and nanometer-scale resolution. Although single-molecule-based methods are becoming increasingly accessible to non-experts, optimizing new single-molecule experiments can be challenging, in particular when super-resolution imaging and tracking are applied to live cells. In this review, we summarize common obstacles to live-cell single-molecule microscopy and describe the methods we have developed and applied to overcome these challenges in live bacteria. We examine the choice of fluorophore and labeling scheme, approaches to achieving single-molecule levels of fluorescence, considerations for maintaining cell viability, and strategies for detecting single-molecule signals in the presence of noise and sample drift. We also discuss methods for analyzing single-molecule trajectories and the challenges presented by the finite size of a bacterial cell and the curvature of the bacterial membrane. PMID:25123183

  20. Reuse of single-dose nevirapine in subsequent pregnancies for the prevention of mother-to-child HIV transmission in Lusaka, Zambia: A cohort study

    Directory of Open Access Journals (Sweden)

    Sinkala Moses

    2008-12-01

    Full Text Available Abstract Background Single-dose nevirapine (SDNVP for the prevention of mother-to-child HIV transmission (PMTCT results in the selection of resistance mutants among HIV-infected mothers. The effects of these mutations on the efficacy of SDNVP use in a subsequent pregnancy are not well understood. Methods We compared risks of perinatal HIV transmission between multiparous women who had previously received a dose of SDNVP (exposed and those that had not (unexposed and who were given SDNVP for the index pregnancy within a PMTCT clinical study. We also compared transmission risks among exposed and unexposed women who had two consecutive pregnancies within the trial. Logistic regression modeling was used to adjust for possible confounders. Results Transmission risks did not differ between 59 SDNVP-exposed and 782 unexposed women in unadjusted analysis or after adjustment for viral load and disease stage (adjusted odds ratio 0.6, 95% confidence interval [CI] 0.2 to 2.0. Among 43 women who had two consecutive pregnancies during the study, transmission risks were 7% (95% CI 1% to 19% at both the first (unexposed and second (exposed delivery. The results were unchanged, if infant death was included as an outcome. Conclusion These data suggest that the efficacy of SDNVP may not be diminished when reused in subsequent pregnancies.

  1. Reuse of single-dose nevirapine in subsequent pregnancies for the prevention of mother-to-child HIV transmission in Lusaka, Zambia: a cohort study.

    Science.gov (United States)

    Walter, Jan; Kuhn, Louise; Kankasa, Chipepo; Semrau, Katherine; Sinkala, Moses; Thea, Donald M; Aldrovandi, Grace M

    2008-12-30

    Single-dose nevirapine (SDNVP) for the prevention of mother-to-child HIV transmission (PMTCT) results in the selection of resistance mutants among HIV-infected mothers. The effects of these mutations on the efficacy of SDNVP use in a subsequent pregnancy are not well understood. We compared risks of perinatal HIV transmission between multiparous women who had previously received a dose of SDNVP (exposed) and those that had not (unexposed) and who were given SDNVP for the index pregnancy within a PMTCT clinical study. We also compared transmission risks among exposed and unexposed women who had two consecutive pregnancies within the trial. Logistic regression modeling was used to adjust for possible confounders. Transmission risks did not differ between 59 SDNVP-exposed and 782 unexposed women in unadjusted analysis or after adjustment for viral load and disease stage (adjusted odds ratio 0.6, 95% confidence interval [CI] 0.2 to 2.0). Among 43 women who had two consecutive pregnancies during the study, transmission risks were 7% (95% CI 1% to 19%) at both the first (unexposed) and second (exposed) delivery. The results were unchanged, if infant death was included as an outcome. These data suggest that the efficacy of SDNVP may not be diminished when reused in subsequent pregnancies.

  2. Surgical Complications after Right Hepatectomy for Live Liver Donation: Largest Single-Center Western World Experience.

    Science.gov (United States)

    Gorgen, Andre; Goldaracena, Nicolas; Zhang, Wei; Rosales, Roizar; Ghanekar, Anand; Lilly, Les; Cattral, Mark; Greig, Paul; McCluskey, Stuart; McGilvray, Ian; Selzner, Nazia; Bhat, Mamatha; Selzner, Markus; Levy, Gary; Grant, David; Sapisochin, Gonzalo

    2018-03-22

    The authors assessed the incidence, management, and risk factors for postoperative complications after right lobe (RL) live donor hepatectomy in a high-volume center in North America. All donors undergoing an RL live donor hepatectomy between 2000 and 2017 at our institution were included. The primary outcome was the development of complications (both medical and surgical). Predictors of postoperative complications were determined by logistic regression. A total of 587 patients underwent RL live donor hepatectomy. Among those, 187 postoperative complications were diagnosed in 141 (24%) patients. One patient had >90-day morbidity, and there were no donor deaths. Overall complications were significantly higher in the first era, 2000 to 2008 (81 [57.4%]) versus the second era, 2009 to 2017 (60 [42.6%]) ( p  = 0.01). On multivariate analysis, the only predictor of postoperative complications was the center volume of RL live donor hepatectomy in the previous 12 months with an odds ratio of 0.97 (95% confidence interval: 0.95-0.99). In conclusion, increasing center volume is associated with lower rates of postoperative complications after RL living liver donation. Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

  3. Exploring transduction mechanisms of protein transduction domains (PTDs) in living cells utilizing single-quantum dot tracking (SQT) technology.

    Science.gov (United States)

    Suzuki, Yasuhiro

    2012-01-01

    Specific protein domains known as protein transduction domains (PTDs) can permeate cell membranes and deliver proteins or bioactive materials into living cells. Various approaches have been applied for improving their transduction efficacy. It is, therefore, crucial to clarify the entry mechanisms and to identify the rate-limiting steps. Because of technical limitations for imaging PTD behavior on cells with conventional fluorescent-dyes, how PTDs enter the cells has been a topic of much debate. Utilizing quantum dots (QDs), we recently tracked the behavior of PTD that was derived from HIV-1 Tat (TatP) in living cells at the single-molecule level with 7-nm special precision. In this review article, we initially summarize the controversy on TatP entry mechanisms; thereafter, we will focus on our recent findings on single-TatP-QD tracking (SQT), to identify the major sequential steps of intracellular delivery in living cells and to discuss how SQT can easily provide direct information on TatP entry mechanisms. As a primer for SQT study, we also discuss the latest findings on single particle tracking of various molecules on the plasma membrane. Finally, we discuss the problems of QDs and the challenges for the future in utilizing currently available QD probes for SQT. In conclusion, direct identification of the rate-limiting steps of PTD entry with SQT should dramatically improve the methods for enhancing transduction efficiency.

  4. Exploring Transduction Mechanisms of Protein Transduction Domains (PTDs in Living Cells Utilizing Single-Quantum Dot Tracking (SQT Technology

    Directory of Open Access Journals (Sweden)

    Yasuhiro Suzuki

    2012-01-01

    Full Text Available Specific protein domains known as protein transduction domains (PTDs can permeate cell membranes and deliver proteins or bioactive materials into living cells. Various approaches have been applied for improving their transduction efficacy. It is, therefore, crucial to clarify the entry mechanisms and to identify the rate-limiting steps. Because of technical limitations for imaging PTD behavior on cells with conventional fluorescent-dyes, how PTDs enter the cells has been a topic of much debate. Utilizing quantum dots (QDs, we recently tracked the behavior of PTD that was derived from HIV-1 Tat (TatP in living cells at the single-molecule level with 7-nm special precision. In this review article, we initially summarize the controversy on TatP entry mechanisms; thereafter, we will focus on our recent findings on single-TatP-QD tracking (SQT, to identify the major sequential steps of intracellular delivery in living cells and to discuss how SQT can easily provide direct information on TatP entry mechanisms. As a primer for SQT study, we also discuss the latest findings on single particle tracking of various molecules on the plasma membrane. Finally, we discuss the problems of QDs and the challenges for the future in utilizing currently available QD probes for SQT. In conclusion, direct identification of the rate-limiting steps of PTD entry with SQT should dramatically improve the methods for enhancing transduction efficiency.

  5. Single-electron transfer living radical copolymerization of SWCNT-g-PMMA via graft from approach

    Czech Academy of Sciences Publication Activity Database

    Jaisankar, S. N.; Haridharan, N.; Murali, A.; Ponyrko, Sergii; Špírková, Milena; Mandal, A. B.; Matějka, Libor

    2014-01-01

    Roč. 55, č. 13 (2014), s. 2959-2966 ISSN 0032-3861 R&D Projects: GA ČR GAP108/12/1459 Institutional support: RVO:61389013 Keywords : single electron transfer * single-walled carbon nanotubes * controlled radical polymerization Subject RIV: CD - Macromolecular Chemistry Impact factor: 3.562, year: 2014

  6. Risk factors for vertical transmission of hepatitis C virus: a single center experience with 710 HCV-infected mothers.

    Science.gov (United States)

    Garcia-Tejedor, Amparo; Maiques-Montesinos, Vicente; Diago-Almela, Vicente José; Pereda-Perez, Antonio; Alberola-Cuñat, Vicente; López-Hontangas, José Luís; Perales-Puchalt, Alfredo; Perales, Alfredo

    2015-11-01

    The aim of this study was to analyze the risk factors on the perinatal transmission of hepatitis C virus (HCV). A retrospective cohort study with 711 infants born to 710 HCV-infected mothers was conducted at the Hospital La Fe, in Valencia, Spain, from 1986 to 2011. As potential risk factors for transmission we analyzed: maternal age, mode of acquisition of HCV infection, HIV co-infection, antiretroviral treatment against HIV, CD4 cell count, HIV and HCV viral load, liver enzyme levels during pregnancy, smoking habit, gestational age, intrapartum invasive procedures, length of rupture of membranes, length of labor, mode of delivery, episiotomy, birth weight, newborn gender and type of feeding. Overall perinatal HCV transmission rate was 2.4%. The significant risk factors related with HCV transmission were maternal virus load >615copies/mL (OR 9.3 [95% CI 1.11-78.72]), intrapartum invasive procedures (OR 10.1 [95% CI 2.6-39.02]) and episiotomy (OR 4.2 [95% CI 1.2-14.16]). HIV co-infection and newborn female were near significance (p=0.081 and 0.075, respectively). Invasive procedures as fetal scalp blood sampling or internal electrode and episiotomy increase vertical transmission of HCV, especially in patients with positive HCV RNA virus load at delivery. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  7. Parenting African American Children With Autism: The Influence of Respect and Faith in Mother, Father, Single-, and Two-Parent Care.

    Science.gov (United States)

    Burkett, Karen; Morris, Edith; Anthony, Jean; Shambley-Ebron, Donna; Manning-Courtney, Patricia

    2016-08-10

    Parents are the most significant contributor to care of children with autism spectrum disorder (ASD), and as such research on African American parenting in ASD is conspicuously absent. Findings relevant to parenting are discussed from a study with urban African American families caring for children with ASD. An ethnonursing study was conducted with 24 African American family members of children with ASD and 28 professionals. Data were analyzed and reported as themes. Two universal themes of were found of respect and faith in God and family that influenced parental care. Two diverse themes of mother's watchful care and father's protective care, along with differences in feelings of isolation and dependence on supports were found among single- and two-parent families. When health care professionals increase their knowledge and understanding of cultural practices in the parental care of children with ASD, they provide health care that is culturally congruent. © The Author(s) 2016.

  8. Quantification of GPCR internalization by single-molecule microscopy in living cells.

    NARCIS (Netherlands)

    Serge, A.; Keijzer, S. de; Hemert, F. Van; Hickman, M.R.; Hereld, D.; Spaink, H.P.; Schmidt, T.; Snaar-Jagalska, B.E.

    2011-01-01

    Receptor internalization upon ligand stimulation is a key component of a cell's response and allows a cell to correctly sense its environment. Novel fluorescent methods have enabled the direct visualization of the agonist-stimulated G-protein-coupled receptors (GPCR) trafficking in living cells.

  9. Identification of Nevirapine-Resistant HIV-1 in the Latent Reservoir after Single-Dose Nevirapine to Prevent Mother-to-Child Transmission of HIV-1

    Science.gov (United States)

    Wind-Rotolo, Megan; Durand, Christine; Cranmer, Lisa; Reid, Alison; Martinson, Neil; Doherty, Meg; Jilek, Benjamin L.; Kagaayi, Joseph; Kizza, Allan; Pillay, Visva; Laeyendecker, Oliver; Reynolds, Steven J.; Eshleman, Susan H.; Lau, Bryan; Ray, Stuart C.; Siliciano, Janet D.; Quinn, Thomas C.; Siliciano, Robert F.

    2009-01-01

    Background Intrapartum single-dose nevirapine decreases mother-to-child transmission of human immunodeficiency virus type 1 (HIV-1) but promotes nevirapine resistance. Although resistant viruses fade to undetectable levels in plasma, they may persist as stably integrated proviruses within the latent reservoir in resting CD4+ T cells, potentially complicating future treatment. Methods Blood samples were collected from 60 women from South Africa and Uganda >6 months after they had received single-dose nevirapine. To selectively analyze the stable latent form of HIV-1, resting CD4+ T cells were isolated and activated in the presence of reverse-transcriptase inhibitors and integrase inhibitors, which allows for the specific isolation of viruses produced by cells with stably integrated proviral DNA. These viruses were then analyzed for nevirapine resistance. Results Although only a small number of latently infected cells were present in each blood sample (mean, 162 cells), nevirapine resistance mutations (K103N and G190A) were detected in the latent reservoir of 4 (8%) of 50 evaluable women. Conclusions A single dose of nevirapine can establish antiretroviral resistance within the latent reservoir. This results in a potentially lifelong risk of reemergence of nevirapine-resistant virus and highlights the need for strategies to prevent transmission that do not compromise successful future treatment. PMID:19338474

  10. Experiences and Motives of Australian Single Mothers by Choice Who Make Early Contact with their Child's Donor Relatives.

    Science.gov (United States)

    Kelly, Fiona J; Dempsey, Deborah J

    2017-01-30

    An increasing number of Australian parents of donor-conceived children are making contact with their child's donor relatives prior to their child reaching the age of majority. This process, often referred to as 'donor linking', can be achieved in Australia through either formal or informal mechanisms. Formal mechanisms exist in three states, each of which has legislation enabling donor linking in certain circumstances. Donor linking may also be achieved through informal mechanisms, such as online donor registries, social media searches, direct-to-consumer genetic testing, and fertility clinics which act as intermediaries between donors and recipients. Drawing on qualitative interview data, this article explores the donor linking practices of twenty-five single women who conceived using donated gametes. The findings suggest that early contact with donors is extremely popular among single women and that, even when formal legislative mechanisms are available, informal linking remains common. © The Author 2017. Published by Oxford University Press.

  11. Experiences and Motives of Australian Single Mothers by Choice who make Early Contact with their Child’s Donor Relatives

    Science.gov (United States)

    Dempsey, Deborah J

    2016-01-01

    Abstract An increasing number of Australian parents of donor-conceived children are making contact with their child’s donor relatives prior to their child reaching the age of majority. This process, often referred to as ‘donor linking’, can be achieved in Australia through either formal or informal mechanisms. Formal mechanisms exist in three states, each of which has legislation enabling donor linking in certain circumstances. Donor linking may also be achieved through informal mechanisms, such as online donor registries, social media searches, direct-to-consumer genetic testing, and fertility clinics which act as intermediaries between donors and recipients. Drawing on qualitative interview data, this article explores the donor linking practices of twenty-five single women who conceived using donated gametes. The findings suggest that early contact with donors is extremely popular among single women and that, even when formal legislative mechanisms are available, informal linking remains common. PMID:28137771

  12. Ultrafast nanolaser device for detecting cancer in a single live cell.

    Energy Technology Data Exchange (ETDEWEB)

    Gourley, Paul Lee; McDonald, Anthony Eugene

    2007-11-01

    Emerging BioMicroNanotechnologies have the potential to provide accurate, realtime, high throughput screening of live tumor cells without invasive chemical reagents when coupled with ultrafast laser methods. These optically based methods are critical to advancing early detection, diagnosis, and treatment of disease. The first year goals of this project are to develop a laser-based imaging system integrated with an in- vitro, live-cell, micro-culture to study mammalian cells under controlled conditions. In the second year, the system will be used to elucidate the morphology and distribution of mitochondria in the normal cell respiration state and in the disease state for normal and disease states of the cell. In this work we designed and built an in-vitro, live-cell culture microsystem to study mammalian cells under controlled conditions of pH, temp, CO2, Ox, humidity, on engineered material surfaces. We demonstrated viability of cell culture in the microsystem by showing that cells retain healthy growth rates, exhibit normal morphology, and grow to confluence without blebbing or other adverse influences of the material surfaces. We also demonstrated the feasibility of integrating the culture microsystem with laser-imaging and performed nanolaser flow spectrocytometry to carry out analysis of the cells isolated mitochondria.

  13. Cost analysis of living donor kidney transplantation in China: a single-center experience.

    Science.gov (United States)

    Zhao, Wenyu; Zhang, Lei; Han, Shu; Zhu, Youhua; Wang, Liming; Zhou, Meisheng; Zeng, Li

    2012-01-01

    Kidney transplantation is the most cost-effective option for the treatment of end-stage renal disease, but the financial aspects of kidney transplantation have not yet been fully investigated. The purpose of this study was to determine the hospital cost of living donor kidney transplantation in China and to identify factors associated with the high cost. Demographic and clinical data of 103 consecutive patients who underwent living donor kidney transplantation from January 2007 to January 2011 at our center were reviewed, and detailed hospital cost of initial admission for kidney transplantation was analyzed. A stepwise multiple regression analysis was computed to determine predictors affecting the total hospital cost. The median total hospital cost was US $10,531, of which 69.2% was for medications, 13.2% for surgical procedures, 11.4% for para clinics, 3.7% for accommodations, 0.5% for nursing care, and 2.0% for other miscellaneous medical services. A multivariate stepwise logistic regression model for overall cost of transplantation revealed that the length of hospital stay, induction therapy, steroid-resistant rejection, maintenance therapy, infection status and body weight were independent predictors affecting the total hospitalization cost. Although the cost of living donor kidney transplantation in China is much lower than that in developed countries, it is a heavy burden for both the government and the patients. As medications formed the greater proportion of the total hospitalization cost, efforts to reduce the cost of drugs should be addressed.

  14. Do singles or couples live healthier lifestyles? Trends in Queensland between 2005-2014.

    Directory of Open Access Journals (Sweden)

    Stephanie Schoeppe

    Full Text Available To compare the frequency of and trends in healthy lifestyle factors between singles and couples.Cross-sectional data from annual surveys conducted from 2005-2014 were used. The pooled sample included 15,001 Australian adults (mean age: 52.9 years, 50% male, 74% couples who participated in the annual Queensland Social Survey via computer-assisted telephone interviews. Relationship status was dichotomised into single and couple. Binary logistic regression was used to assess associations between relationship status, and the frequency of and trends in healthy lifestyle factors.Compared to singles, couples were significantly more likely to be a non-smoker (OR = 1.82, and meet recommendations for limited fast food (OR = 1.12, alcohol consumption (OR = 1.27 and fruit and vegetable intake (OR = 1.24. Fruit and vegetable intake was not significantly associated with relationship status after adjusting for the other healthy lifestyle factors. Conversely, couples were significantly less likely to be within a normal weight range (OR = 0.81. In both singles and couples, the trend data revealed significant declines in the rates of normal weight (singles: OR = 0.97, couples: OR = 0.97 and viewing TV for less than 14 hours per week (singles: OR = 0.85, couples: OR = 0.84, whilst non-smoking rates significantly increased (singles: OR = 1.12, couples: OR = 1.03. The BMI trend was no longer significant when adjusting for health behaviours. Further, in couples, rates of meeting recommendations for physical activity and fruit/vegetable consumption significantly decreased (OR = 0.97 and OR = 0.95, respectively, as did rates of eating no fast food (OR = 0.96. These trends were not significant when adjusting for the other healthy lifestyle factors. In singles, rates of meeting alcohol recommendations significantly increased (OR = 1.08.Health behaviour interventions are needed in both singles and couples, but relationship status needs to be considered in interventions

  15. Do singles or couples live healthier lifestyles? Trends in Queensland between 2005-2014.

    Science.gov (United States)

    Schoeppe, Stephanie; Vandelanotte, Corneel; Rebar, Amanda L; Hayman, Melanie; Duncan, Mitch J; Alley, Stephanie J

    2018-01-01

    To compare the frequency of and trends in healthy lifestyle factors between singles and couples. Cross-sectional data from annual surveys conducted from 2005-2014 were used. The pooled sample included 15,001 Australian adults (mean age: 52.9 years, 50% male, 74% couples) who participated in the annual Queensland Social Survey via computer-assisted telephone interviews. Relationship status was dichotomised into single and couple. Binary logistic regression was used to assess associations between relationship status, and the frequency of and trends in healthy lifestyle factors. Compared to singles, couples were significantly more likely to be a non-smoker (OR = 1.82), and meet recommendations for limited fast food (OR = 1.12), alcohol consumption (OR = 1.27) and fruit and vegetable intake (OR = 1.24). Fruit and vegetable intake was not significantly associated with relationship status after adjusting for the other healthy lifestyle factors. Conversely, couples were significantly less likely to be within a normal weight range (OR = 0.81). In both singles and couples, the trend data revealed significant declines in the rates of normal weight (singles: OR = 0.97, couples: OR = 0.97) and viewing TV for less than 14 hours per week (singles: OR = 0.85, couples: OR = 0.84), whilst non-smoking rates significantly increased (singles: OR = 1.12, couples: OR = 1.03). The BMI trend was no longer significant when adjusting for health behaviours. Further, in couples, rates of meeting recommendations for physical activity and fruit/vegetable consumption significantly decreased (OR = 0.97 and OR = 0.95, respectively), as did rates of eating no fast food (OR = 0.96). These trends were not significant when adjusting for the other healthy lifestyle factors. In singles, rates of meeting alcohol recommendations significantly increased (OR = 1.08). Health behaviour interventions are needed in both singles and couples, but relationship status needs to be considered in interventions

  16. Organohalogen compounds in human breast milk from mothers living in Payatas and Malate, the Philippines: Levels, accumulation kinetics and infant health risk

    Energy Technology Data Exchange (ETDEWEB)

    Malarvannan, Govindan; Kunisue, Tatsuya; Isobe, Tomohiko; Sudaryanto, Agus; Takahashi, Shin [Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577, Ehime Prefecture (Japan); Prudente, Maricar [Science Education Department, De La Salle University, Manila (Philippines); Subramanian, Annamalai [Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577, Ehime Prefecture (Japan); Tanabe, Shinsuke, E-mail: shinsuke@agr.ehime-u.ac.j [Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577, Ehime Prefecture (Japan)

    2009-06-15

    Human breast milk samples (n = 33) from primipara and multipara mothers from Payatas a waste dump site, and Malate a reference site in the Phillipines were collected in 2004 and analyzed for eight organohalogen compounds, viz., PCBs, DDTs, CHLs, HCHs, HCB, TCPMe, PBDEs and HBCDs. DDTs and PCBs were predominant in all the samples. Overall mean concentrations of PBDEs found in our study were higher (7.5 ng/g lipid wt.) than those reported for Japan and many other Asian countries. Primipara mothers had significantly higher levels of DDTs, CHLs and HCHs than multipara mothers, but not PBDEs and HBCDs. A few individuals accumulated CHLs close to or even higher than the tolerable daily intake guidelines proposed by Health Canada. - First comprehensive study on organohalogen contaminants in human breast milk from the Philippines.

  17. Living With Cancer Alone? The Experiences of Singles Diagnosed With Colorectal Cancer.

    Science.gov (United States)

    Tan, Pei Yi; Tien Tau, Loi Carol; Lai Meng, Ow Yong

    2015-01-01

    This paper seeks to understand the experiences of single colorectal cancer patients. This study consisted of 12 semi-structured interviews that were digitally voice-recorded, transcribed, and analyzed. Six main themes emerged: (a) gradual shift in view of cancer diagnosis from fatalistic to normalized, (b) perception of cancer as a nadir experience, (c) concerns of singlehood, (d) factors influencing cancer experiences, (e) factors influencing coping with cancer, and (f) range of responses towards cancer diagnosis. Singles with colorectal cancer require short- to long-term individualized care plans, and psycho-emotional support. This may help enhance their individual coping and adjustment to the diagnosis.

  18. Single molecule experimentation in biological physics: exploring the living component of soft condensed matter one molecule at a time.

    Science.gov (United States)

    Harriman, O L J; Leake, M C

    2011-12-21

    The soft matter of biological systems consists of mesoscopic length scale building blocks, composed of a variety of different types of biological molecules. Most single biological molecules are so small that 1 billion would fit on the full-stop at the end of this sentence, but collectively they carry out the vital activities in living cells whose length scale is at least three orders of magnitude greater. Typically, the number of molecules involved in any given cellular process at any one time is relatively small, and so real physiological events may often be dominated by stochastics and fluctuation behaviour at levels comparable to thermal noise, and are generally heterogeneous in nature. This challenging combination of heterogeneity and stochasticity is best investigated experimentally at the level of single molecules, as opposed to more conventional bulk ensemble-average techniques. In recent years, the use of such molecular experimental approaches has become significantly more widespread in research laboratories around the world. In this review we discuss recent experimental approaches in biological physics which can be applied to investigate the living component of soft condensed matter to a precision of a single molecule. © 2011 IOP Publishing Ltd Printed in the UK & the USA

  19. Taking the ruler to the jungle: single-molecule FRET for understanding biomolecular structure and dynamics in live cells.

    Science.gov (United States)

    Sustarsic, Marko; Kapanidis, Achillefs N

    2015-10-01

    Single-molecule Förster resonance energy transfer (smFRET) serves as a molecular ruler that is ideally posed to study static and dynamic heterogeneity in living cells. Observing smFRET in cells requires appropriately integrated labeling, internalization and imaging strategies, and significant progress has been made towards that goal. Pioneering studies have demonstrated smFRET detection in both prokaryotic and eukaryotic systems, using both wide-field and confocal microscopies, and have started to answer exciting biological questions. We anticipate that future technical developments will open the door to smFRET for the study of structure, conformational changes and kinetics of biomolecules in living cells. Copyright © 2015 Elsevier Ltd. All rights reserved.

  20. Quantitative and real-time effects of carbon quantum dots on single living HeLa cell membrane permeability.

    Science.gov (United States)

    Kong, Weiqian; Liu, Juan; Liu, Ruihua; Li, Hao; Liu, Yang; Huang, Hui; Li, Kunyang; Liu, Jian; Lee, Shuit-Tong; Kang, Zhenhui

    2014-05-21

    The interaction between carbon quantum dots (CQDs) and a single living cell was explored in real time. Here, we provide the quantitative data on the permeability of the HeLa cell membrane in the presence of CQDs with different surface functional groups (CQDs terminated with -OH/-COOH (CQD-OH), -PEG (CQD-PEG), and -NH2 (CQD-NH2)). Although these CQDs have very low toxicity towards HeLa cells, they still increase the cell membrane permeability by 8%, 13%, and 19% for CQD-PEG, CQD-OH, and CQD-NH2, respectively, and this kind of permeability was irreversible. These observations are valuable for promoting the bio-applications of carbon nanostructures in living systems.

  1. Impact of adulthood trauma on homeless mothers.

    Science.gov (United States)

    Zlotnick, Cheryl; Tam, Tammy; Bradley, Kimberly

    2007-02-01

    Using the National Survey of Homeless Assistance Providers and Clients (NSHAPC), we found that among homeless mothers (n = 588), those living without their children were more likely to: be older than 35 years, unmarried, have been incarcerated, have been homeless for at least 1 year, and to have used psychiatric medication. Many homeless mothers had histories of childhood trauma, but it was the accumulation of adulthood traumas that was associated with not living with one's children. Without mental health treatment, younger homeless mothers living with their children today may become the homeless mothers living without their children in the future.

  2. A single-cell scraper based on an atomic force microscope for detaching a living cell from a substrate

    Energy Technology Data Exchange (ETDEWEB)

    Iwata, Futoshi, E-mail: iwata.futoshi@shizuoka.ac.jp [Department of Mechanical Engineering, Faculty of Engineering, Shizuoka University, Johoku, Naka-ku, Hamamatsu 432-8561 (Japan); Research Institute of Electronics, Shizuoka University, Johoku, Naka-ku, Hamamatsu 432-8011 (Japan); Adachi, Makoto; Hashimoto, Shigetaka [Department of Mechanical Engineering, Faculty of Engineering, Shizuoka University, Johoku, Naka-ku, Hamamatsu 432-8561 (Japan)

    2015-10-07

    We describe an atomic force microscope (AFM) manipulator that can detach a single, living adhesion cell from its substrate without compromising the cell's viability. The micrometer-scale cell scraper designed for this purpose was fabricated from an AFM micro cantilever using focused ion beam milling. The homemade AFM equipped with the scraper was compact and standalone and could be mounted on a sample stage of an inverted optical microscope. It was possible to move the scraper using selectable modes of operation, either a manual mode with a haptic device or a computer-controlled mode. The viability of the scraped single cells was evaluated using a fluorescence dye of calcein-acetoxymethl ester. Single cells detached from the substrate were collected by aspiration into a micropipette capillary glass using an electro-osmotic pump. As a demonstration, single HeLa cells were selectively detached from the substrate and collected by the micropipette. It was possible to recultivate HeLa cells from the single cells collected using the system.

  3. Long-term safety in living kidney donors for paediatric transplantation. Single-centre prospective study.

    Science.gov (United States)

    Martin Benlloch, J; Román Ortiz, E; Mendizabal Oteiza, S

    There is enough evidence concerning the short-term safety of living donors after kidney transplantation. However, long-term complications continue to be studied, with a particular interest in young donors. Previous studies have been conducted in older donors for adult renal patients. We present a study of long-term complications in kidney donors for our paediatric population. We carried out a long-term donor study for the 54 living kidney-donor transplantations performed at our department from 1979 to June 2014. We monitored the glomerular filtration rate (GFR) on the basis of 24-hour urine creatinine clearance, 24-hour proteinuria and the development of arterial hypertension in the 48 donors who were followed up for more than one year. Only the 39 patients who were exclusively followed up by our department have been included in the results analysis. GFR through creatinine clearance was stable after an initial decrease. No proteinuria was observed in any of the cases. One patient developed chronic kidney disease (CKD), which resulted in a cumulative incidence of 2%. GFR below 60mL/min/1.73 m 2 was not reported in any other patients. Arterial hypertension was diagnosed in 25% of donors, 90% of which were treated with antihypertensives. Risk of CKD and hypertension in living kidney donors for paediatric recipients, who are carefully monitored throughout their evolution, is similar to that of the general population. Therefore, this technique appears to be safe in both the short and long term. Copyright © 2016 Sociedad Española de Nefrología. Published by Elsevier España, S.L.U. All rights reserved.

  4. Living with half a heart - experiences of young adults with single ventricle physiology

    DEFF Research Database (Denmark)

    Overgaard, Dorthe; King, Catriona; Christensen, Rie F

    2013-01-01

    BACKGROUND AND RESEARCH OBJECTIVE:: Approximately 3% of children with congenital heart disease born in Denmark have single ventricle physiology (SVP). In previous decades, these children did not survive into adulthood. However, because of new surgical techniques and improved medical care, they now...

  5. Helical CT angiography : A single imaging modality to evaluate a live renal donor

    Directory of Open Access Journals (Sweden)

    Waheed Zaman

    2002-01-01

    Conclusions: Helical CT angiography, which is highly specific for arterial and venous anatomy as well as other anatomical and functional details, can become the single imaging modality for preoperative assessment of poten-tial donors in place of conventional angiography and IVU. CTA, being minimally invasive and cost effective, is well accepted by a normal healthy donor

  6. HIGH-SPEED SINGLE QUANTUM DOT IMAGING OF IN LIVE CELLS REVEAL HOP DIFFUSION

    DEFF Research Database (Denmark)

    Lagerholm, B. Christoffer; Clausen, Mathias P.

    2011-01-01

    Ultra high-speed single particle tracking (image frame rates 40-50 kHz) experiments with 40 nm gold particles has indicated that lipids and proteins in the plasma membrane undergo hop-diffusion between nanometer sized compartments (Fujiwara et al. (2002) J Cell Biol. 157:1071-81). These findings...... have yet to be independently confirmed. In this work, we show that high-speed single particle tracking with quantum dots (QDs) and using a standard wide-field fluorescence microscope and an EMCCD is possible at image acquisition rates of up to ~2000 Hz. The spatial precision in these experiments is ~40...... nm (as determined from the standard deviation of repeated position measurements of an immobile QD on a cell). Using this system, we show that membrane proteins and lipids, which have been exogenously labeled with functionalized QDs, show examples of three types of motion in the plasma membrane...

  7. EDUCATIONAL TACTICS OF MOTHERS AND THEIR RELATION TO THE PSYCHOLOGICAL CHARACTERISTICS OF ADOLESCENTS IN TWO-PARENT AND SINGLE-PARENT FAMILIES

    Directory of Open Access Journals (Sweden)

    Zuev Konstantin Borisovich

    2013-03-01

    Full Text Available The paper presents the results of a study of the psychological characteristics of boys and girls from complete and incomplete families. In addition to the type of family, the authors consider the educational tactics of mothers. The combined effects of the type of family and educational tactics on psychological characteristics of the adolescent were investigated. Adolescence was chosen as a period, when on the one hand, a relatively stable personality structure is developed, and on the other hand, the importance of the immediate social environment is extremely high. For our study we selected the psychological characteristics, to the utmost revealing the reaction of children to their parents' divorce: the level of subjective control (degree of responsibility for their own lives, and the sovereignty of the psychological space (clearnesse of psychological boundaries. It is shown that the largest influence on the psychological characteristics of adolescents, regardless of the type of family, is rendered by hostility in maternal education. The dependence of psychological characteristics of maternal directiveness occurs only at high values ​​of such educational tactics that highlights the consistency of the obtained results with the classical

  8. Monofunctional stealth nanoparticle for unbiased single molecule tracking inside living cells.

    Science.gov (United States)

    Lisse, Domenik; Richter, Christian P; Drees, Christoph; Birkholz, Oliver; You, Changjiang; Rampazzo, Enrico; Piehler, Jacob

    2014-01-01

    On the basis of a protein cage scaffold, we have systematically explored intracellular application of nanoparticles for single molecule studies and discovered that recognition by the autophagy machinery plays a key role for rapid metabolism in the cytosol. Intracellular stealth nanoparticles were achieved by heavy surface PEGylation. By combination with a generic approach for nanoparticle monofunctionalization, efficient labeling of intracellular proteins with high fidelity was accomplished, allowing unbiased long-term tracking of proteins in the outer mitochondrial membrane.

  9. Exsolution lamellae in volcanic pyroxene; Single phenocryst thermometry for long-lived magmatic reservoir

    Science.gov (United States)

    I Made, R.; Herrin, J. S.; Tay, Y. Y.; Costa Rodriguez, F.

    2017-12-01

    Comprehensive understanding of the relevant timescales of thermal and chemical evolution of magma below the active volcanoes can help us to better anticipate volcanic eruptions and their likely precursor signals. In recent years, several lines of thermochronological inquiry have converged on a realization that, within many volcanic systems, magmas experience prolonged periods of relatively low-temperature storage prior to eruption during short duration transient events. This prolonged storage at low magmatic temperatures can result in series of solid state phase transformations within minerals, producing a petrologic record of their thermal history. In this example, we observed pigeonite exsolution lamellae in augite phenocrysts from the 2011 eruption of Cordon Caulle volcano, Chile. The small size of these features ( 70nm width and bear exsolution textures and apply this knowledge to understanding the thermal conditions of magma storage in long-lived volcanic reservoirs.

  10. Multiparametric atomic force microscopy imaging of single bacteriophages extruding from living bacteria

    Science.gov (United States)

    Alsteens, David; Trabelsi, Heykel; Soumillion, Patrice; Dufrêne, Yves F.

    2013-12-01

    Force-distance (FD) curve-based atomic force microscopy is a valuable tool to simultaneously image the structure and map the biophysical properties of biological samples at the nanoscale. Traditionally, FD-based atomic force microscopy has been severely limited by its poor temporal and lateral resolutions. Here we report the use of advanced FD-based technology combined with biochemically sensitive tips to image filamentous bacteriophages extruding from living bacteria at unprecedented speed and resolution. Directly correlated multiparametric images of the structure, adhesion and elasticity of infected bacteria demonstrate that the sites of assembly and extrusion localize at the bacterial septum in the form of soft nanodomains surrounded by stiff cell wall material. The quantitative nano-bio-imaging method presented here offers a wealth of opportunities for mapping the physical properties and molecular interactions of complex biosystems, from viruses to tissues.

  11. Fluorescence molecule counting for single-molecule studies in crowded environment of living cells without and with broken ergodicity.

    Science.gov (United States)

    Földes-Papp, Zeno; Baumann, Gerd

    2011-05-01

    We present a new approach to distinguish between non-ergodic and ergodic behavior. Performing ensemble averaging in a subpopulation of individual molecules leads to a mean value that can be similar to the mean value obtained in an ergodic system. The averaging is carried out by minimizing the variation between the sum of the temporal averaged mean square deviation of the simulated data with respect to the logarithmic scaling behavior of the subpopulation. For this reason, we first introduce a kind of Continuous Time Random Walks (CTRW), which we call Limited Continuous Time Random Walks (LCTRW) on fractal support. The random waiting time distributions are sampled at points which fulfill the condition N <1, where N is the Poisson probability of finding a single molecule in the femtoliter-sized observation volume ΔV at the single-molecule level. Given a subpopulation of different single molecules of the same kind, the ratio T/ T(m) between the measurement time T and the meaningful time T(m), which is the time for observing just one and the same single molecule, is the experimentally accessible quantity that allows to compare different molecule numbers in the subpopulation. In addition, the mean square displacement traveled by the molecule during the time t is determined by an upper limit of the geometric dimension of the living cell or its nucleus.

  12. Subunit rotation in a single FoF1-ATP synthase in a living bacterium monitored by FRET

    Science.gov (United States)

    Seyfert, K.; Oosaka, T.; Yaginuma, H.; Ernst, S.; Noji, H.; Iino, R.; Börsch, M.

    2011-03-01

    FoF1-ATP synthase is the ubiquitous membrane-bound enzyme in mitochondria, chloroplasts and bacteria which provides the 'chemical energy currency' adenosine triphosphate (ATP) for cellular processes. In Escherichia coli ATP synthesis is driven by a proton motive force (PMF) comprising a proton concentration difference ΔpH plus an electric potential ΔΨ across the lipid membrane. Single-molecule in vitro experiments have confirmed that proton-driven subunit rotation within FoF1-ATP synthase is associated with ATP synthesis. Based on intramolecular distance measurements by single-molecule fluorescence resonance energy transfer (FRET) the kinetics of subunit rotation and the step sizes of the different rotor parts have been unraveled. However, these experiments were accomplished in the presence of a PMF consisting of a maximum ΔpH ~ 4 and an unknown ΔΨ. In contrast, in living bacteria the maximum ΔpH across the plasma membrane is likely 0.75, and ΔΨ has been measured between -80 and -140 mV. Thus the problem of in vivo catalytic turnover rates, or the in vivo rotational speed in single FoF1-ATP synthases, respectively, has to be solved. In addition, the absolute number of functional enzymes in a single bacterium required to maintain the high ATP levels has to be determined. We report our progress of measuring subunit rotation in single FoF1-ATP synthases in vitro and in vivo, which was enabled by a new labeling approach for single-molecule FRET measurements.

  13. 75 FR 26875 - Mother's Day, 2010

    Science.gov (United States)

    2010-05-12

    ... honoring the influence of mothers on our lives and communities. In the ensuing decades, many Americans... made us who we are. As we honor today's mothers, we also reflect upon the memory of those who have.... Let us express our deepest love and thanks to our mothers and remember those who, though no longer...

  14. Correlation of Dual Colour Single Particle Trajectories for Improved Detection and Analysis of Interactions in Living Cells

    Directory of Open Access Journals (Sweden)

    Kevin Braeckmans

    2013-08-01

    Full Text Available Interactions between objects inside living cells are often investigated by looking for colocalization between fluorescence microscopy images that are recorded in separate colours corresponding to the fluorescent label of each object. The fundamental limitation of this approach in the case of dynamic objects is that coincidental colocalization cannot be distinguished from true interaction. Instead, correlation between motion trajectories obtained by dual colour single particle tracking provides a much stronger indication of interaction. However, frequently occurring phenomena in living cells, such as immobile phases or transient interactions, can limit the correlation to small parts of the trajectories. The method presented here, developed for the detection of interaction, is based on the correlation inside a window that is scanned along the trajectories, covering different subsets of the positions. This scanning window method was validated by simulations and, as an experimental proof of concept, it was applied to the investigation of the intracellular trafficking of polymeric gene complexes by endosomes in living retinal pigment epithelium cells, which is of interest to ocular gene therapy.

  15. Single Particle Tracking reveals two distinct environments for CD4 receptors at the surface of living T lymphocytes

    International Nuclear Information System (INIS)

    Mascalchi, Patrice; Lamort, Anne Sophie; Salomé, Laurence; Dumas, Fabrice

    2012-01-01

    Highlights: ► We studied the diffusion of single CD4 receptors on living lymphocytes. ► This study reveals that CD4 receptors have either a random or confined diffusion. ► The dynamics of unconfined CD4 receptors was accelerated by a temperature raise. ► The dynamics of confined CD4 receptors was unchanged by a temperature raise. ► Our results suggest the existence of two different environments for CD4 receptors. -- Abstract: We investigated the lateral diffusion of the HIV receptor CD4 at the surface of T lymphocytes at 20 °C and 37 °C by Single Particle Tracking using Quantum Dots. We found that the receptors presented two major distinct behaviors that were not equally affected by temperature changes. About half of the receptors showed a random diffusion with a diffusion coefficient increasing upon raising the temperature. The other half of the receptors was permanently or transiently confined with unchanged dynamics on raising the temperature. These observations suggest that two distinct subpopulations of CD4 receptors with different environments are present at the surface of living T lymphocytes.

  16. [Incidence of fetal macrosomia among single live birth neonates and influencing factors in Xi' an, 2010-2013].

    Science.gov (United States)

    Zhang, Q; Bai, R H; Wang, L L; Dang, S N; Mi, B B; Yan, H

    2016-08-10

    To analyze the incidence and influencing factors on fetal macrosomia among single live birth neonates in Xi' an. A questionnaire survey was conducted among women at the childbearing age who were selected through multi stage stratified random sampling in Xi 'an during 2010-2013. All the childbearing aged women involved, were in pregnancy or having definite pregnancy outcomes. A total of 4 970 women at childbearing age and their infants were investigated. The overall incidence of fetal macrosomia weight among the single live birth neonates under study, was 9.7% during 2010-2013 (8.9% in 2010, 8.1% in 2011, 10.0% in 2012 and 10.1% in 2013, respectively). The incidence rates of fetal macrosomia appeared 10.5% in the central district and, 8.6% in the rural-urban area of Xi'an. There were statistically significant differences (Pmacrosomia. The incidence of fetal macrosomia in Xi' an was higher than the national figures. The incidence of fetal macrosomia was higher in the central district than in rural-urban area. Having male neonate, postmature birth, gestational diabetes, being multipara, drinking during pregnancy were the risk factors related to fetal macrosomia.

  17. Time-Lapse Monitoring of DNA Damage Colocalized With Particle Tracks in Single Living Cells

    Energy Technology Data Exchange (ETDEWEB)

    McFadden, Conor H. [Department of Radiation Physics, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Hallacy, Timothy M. [Department of Radiation Physics, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Department of Physics and Astronomy, Rice University, Houston, Texas (United States); Flint, David B. [Department of Radiation Physics, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Graduate School of Biomedical Sciences, The University of Texas, Houston, Texas (United States); Granville, Dal A. [Department of Medical Physics, The Ottawa Hospital Cancer Centre, Ottawa, Ontario (Canada); Asaithamby, Aroumougame [Division of Molecular Radiation Biology, Department of Radiation Oncology, University of Texas Southwestern Medical Centre, Dallas, Texas (United States); Sahoo, Narayan [Department of Radiation Physics, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Akselrod, Mark S. [Crystal Growth Division, Landauer, Inc, Stillwater, Oklahoma (United States); Sawakuchi, Gabriel O., E-mail: gsawakuchi@mdanderson.org [Department of Radiation Physics, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Graduate School of Biomedical Sciences, The University of Texas, Houston, Texas (United States)

    2016-09-01

    Purpose: Understanding the DNA damage and repair induced by hadron therapy (HT) beams is crucial for developing novel strategies to maximize the use of HT beams to treat cancer patients. However, spatiotemporal studies of DNA damage and repair for beam energies relevant to HT have been challenging. We report a technique that enables spatiotemporal measurement of radiation-induced damage in live cells and colocalization of this damage with charged particle tracks over a broad range of clinically relevant beam energies. The technique uses novel fluorescence nuclear track detectors with fluorescence confocal laser scanning microscopy in the beam line to visualize particle track traversals within the subcellular compartments of live cells within seconds after injury. Methods and Materials: We designed and built a portable fluorescence confocal laser scanning microscope for use in the beam path, coated fluorescence nuclear track detectors with fluorescent-tagged live cells (HT1080 expressing enhanced green fluorescent protein tagged to XRCC1, a single-strand break repair protein), placed the entire assembly into a proton therapy beam line, and irradiated the cells with a fluence of ∼1 × 10{sup 6} protons/cm{sup 2}. Results: We successfully obtained confocal images of proton tracks and foci of DNA single-strand breaks immediately after irradiation. Conclusions: This technique represents an innovative method for analyzing biological responses in any HT beam line at energies and dose rates relevant to therapy. It allows precise determination of the number of tracks traversing a subcellular compartment and monitoring the cellular damage therein, and has the potential to measure the linear energy transfer of each track from therapeutic beams.

  18. [Evaluation of empowerment among socially disadvantaged women - examination in different living circumstances].

    Science.gov (United States)

    Sperlich, S

    2010-07-01

    This paper follows on from a previous study which assessed the relationship between socioeconomic position, empowerment and the development of psychological health in women after treatment in mother-child rehabilitation centres in Germany. The study revealed that socioeconomic position was less important for mothers caring for young children. For this reason the present study is based on a broader definition of social inequity, taking household conditions and psychosocial stressors into account. The aim of the paper is to answer the following questions: 1) To what extent does the improvement of psychological health depend on the living circumstances of the mothers? 2) What is the impact of living conditions on the success of empowerment? 3) Does the health-related impact of empowerment differ between different living conditions of the mothers? By conducting a cluster analysis on clinical data of the women (n=6094), seven different living circumstances of the mothers could be detected. Two living circumstances could be identified to be related to extremely poor health. These are 'dissatisfied single mothers with high degrees of psychosocial distress and lack of social support', and 'married mothers with conflicts within the family and self-perceived lack of appreciation'. At the end of inpatient treatment these mothers showed the highest reduction of psychological symptoms, but after six and twelve months the symptoms increased again. The results of empowerment showed that empowerment is most health-effective for mothers living in poor living conditions, but the success of empowerment here is less pronounced. As a consequence the health effect of empowerment was smaller for those mothers. The study suggests that health promotion programmes could be more effective when they explicitly take the living circumstances of their participants into account. Copyright Georg Thieme Verlag KG Stuttgart . New York.

  19. Non-invasive single-cell biomechanical analysis using live-imaging datasets.

    Science.gov (United States)

    Pearson, Yanthe E; Lund, Amanda W; Lin, Alex W H; Ng, Chee P; Alsuwaidi, Aysha; Azzeh, Sara; Gater, Deborah L; Teo, Jeremy C M

    2016-09-01

    The physiological state of a cell is governed by a multitude of processes and can be described by a combination of mechanical, spatial and temporal properties. Quantifying cell dynamics at multiple scales is essential for comprehensive studies of cellular function, and remains a challenge for traditional end-point assays. We introduce an efficient, non-invasive computational tool that takes time-lapse images as input to automatically detect, segment and analyze unlabeled live cells; the program then outputs kinematic cellular shape and migration parameters, while simultaneously measuring cellular stiffness and viscosity. We demonstrate the capabilities of the program by testing it on human mesenchymal stem cells (huMSCs) induced to differentiate towards the osteoblastic (huOB) lineage, and T-lymphocyte cells (T cells) of naïve and stimulated phenotypes. The program detected relative cellular stiffness differences in huMSCs and huOBs that were comparable to those obtained with studies that utilize atomic force microscopy; it further distinguished naïve from stimulated T cells, based on characteristics necessary to invoke an immune response. In summary, we introduce an integrated tool to decipher spatiotemporal and intracellular dynamics of cells, providing a new and alternative approach for cell characterization. © 2016. Published by The Company of Biologists Ltd.

  20. Predictors of Human Papillomavirus Vaccine Initiation and Completion Among Latino Mothers of 11- to 17-Year-Old Daughters Living Along the Texas-Mexico Border.

    Science.gov (United States)

    Morales-Campos, Daisy Y; Parra-Medina, Deborah

    Few Texas Latino girls initiate and complete the human papillomavirus (HPV) vaccine series, but few studies have examined predictors of initiation and completion in this group. Mothers are crucial to vaccine uptake. Using self-reported data from mothers of unvaccinated girls (n = 317), we examined the association between predictors (HPV and HPV vaccine knowledge, vaccine self-efficacy) and outcomes (initiation and completion). Despite an increase in HPV and HPV vaccine knowledge from baseline to follow-up (n = 195), we found no association between the predictors and the outcomes. Findings showed that health insurance status and study group participation (Entre Madre e Hija program or brochure only) were associated with initiation and completion.

  1. Teenage mothers.

    Science.gov (United States)

    Marino, Jennifer L; Lewis, Lucy N; Bateson, Deborah; Hickey, Martha; Skinner, S Rachel

    2016-10-01

    Australia's teenage birth rate has fallen to historic lows, but teenage motherhood still occurs and can be challenging for mother and baby. The aim of this article is to review current evidence on the epidemiology and clinical care of teenage pregnancy and parenting, and provide recommendations around management of these young people in Australia. Teenage mothers may have experienced family, sexual, and partner violence, family disruption, and socioeconomic disadvantage. Outcomes on a range of peripartum measures are worse for teenage mothers and their babies. Longer term risks for the mother include depression and rapid repeat pregnancy; for the child, intergenerational teenage parenthood; and for both, socioeconomic disadvantage. Teenage motherhood occurs more often within communities where poverty, Aboriginal and Torres Strait Islander status and rural/remote location intersect. General practitioners play a critical role in identification of at-risk teens, preventing unintended teenage pregnancy, clinical care of pregnant teens, and promoting the health and wellbeing of teenage mothers and their children.

  2. Medida de energia total do leite materno de mães de trigêmeos, gêmeos e filhos únicos Total energy supplied by the human milk from mothers of triplet, twin and single neonates

    Directory of Open Access Journals (Sweden)

    Ariane Nadólskis Severine

    2008-06-01

    Full Text Available OBJETIVO: Comparar a energia total do leite materno de mães de trigêmeos, gêmeos e filhos únicos. MÉTODOS: Estudo transversal e controlado feito com mães usuárias do banco de leite do Hospital Israelita Albert Einstein. Foram analisadas seis amostras de leite materno de mães de trigêmeos, 13 de gêmeos e 17 de filhos únicos, coletadas sob paramentação, conforme normas técnicas da Rede Brasileira de Bancos de Leite Humano. A análise foi realizada por meio do teste de crematócrito e os resultados submetidos à análise de variância de Kuskal-Wallis. RESULTADOS: A energia total do leite materno de mães de trigêmeos apresentou mediana de calorias maior (651kcal/L, quando comparada à do leite de mães de filhos únicos (560kcal/L (pOBJECTIVE: To compare the total energy of the human milk of mothers of triplets, twins and single-children. METHODS: A cross-sectional and controlled study with mothers from the Human Milk Bank of Hospital Israelita Albert Einstein was performed. Six samples of triplet, 13 of twin and 17 of only-child mother's milk were analyzed. The milk samples were collected according to the Brazilian Human Milk Banks Network techniques and procedures and were analyzed by creamatocrit method. The results were compared by Kruskal-Wallis variance test. RESULTS: The total milk energy from triplets mothers was statistically higher (median - 651kcal/L than single-child mothers (560kcal/L (p<0.05. The milk of twin mothers and of single child mothers, regarding energy content, were similar. The triplet neonates presented lower birth weight (median-1344g when compared to single neonates (1975g (p<0.01. There was no significant difference among the studied groups regarding maternal age and gestational age. CONCLUSIONS: The present study, the first addressing this subject in literature, indicates a higher value of total energy in milk from triplets' mothers. However, further studies are necessary to clarify the mechanisms of

  3. Timely referral saves the lives of mothers and newborns: Midwifery led continuum of care in marginalized teagarden communities – A qualitative case study in Bangladesh [version 1; referees: 1 approved, 2 approved with reservations

    Directory of Open Access Journals (Sweden)

    Animesh Biswas

    2018-03-01

    Full Text Available Background: Prompt and efficient identification, referral of pregnancy related complications and emergencies are key factors to the reduction of maternal and newborn morbidity and mortality. As a response to this critical need, a midwifery led continuum of reproductive health care was introduced in five teagardens in the Sylhet division, Bangladesh during 2016. Within this intervention, professional midwives provided reproductive healthcare to pregnant teagarden women in the community.  This study evaluates the effect of the referral of pregnancy related complications. Methods: A qualitative case study design by reviewing records retrospectively was used to explore the effect of deploying midwives on referrals of pregnancy related complications from the selected teagardens to the referral health facilities in Moulvibazar district of the Sylhet division during 2016.  In depth analyses was also performed on 15 randomly selected cases to understand the facts behind the referral. Results: Out of a total population of 450 pregnant women identified by the midwives, 72 complicated mothers were referred from the five teagardens to the facilities. 76.4% of mothers were referred to conduct delivery at facilities, and 31.1% of them were referred with the complication of prolonged labour. Other major complications were pre-eclampsia (17.8%, retention of the placenta with post-partum hemorrhage (11.1% and premature rupture of the membrane (8.9%. About 60% of complicated mothers were referred to the primary health care centre, and among them 14% of mothers were delivered by caesarean section. 94% deliveries resulted in livebirths and only 6% were stillbirths. Conclusions: This study reveals that early detection of pregnancy complications by skilled professionals and timely referral to a facility is beneficial in saving the majority of baby’s as well as mother’s lives in resource-poor teagardens with a considerable access barrier to health facilities.

  4. Stable single-unit-cell nanosheets of zeolite MFI as active and long-lived catalysts.

    Science.gov (United States)

    Choi, Minkee; Na, Kyungsu; Kim, Jeongnam; Sakamoto, Yasuhiro; Terasaki, Osamu; Ryoo, Ryong

    2009-09-10

    Zeolites-microporous crystalline aluminosilicates-are widely used in petrochemistry and fine-chemical synthesis because strong acid sites within their uniform micropores enable size- and shape-selective catalysis. But the very presence of the micropores, with aperture diameters below 1 nm, often goes hand-in-hand with diffusion limitations that adversely affect catalytic activity. The problem can be overcome by reducing the thickness of the zeolite crystals, which reduces diffusion path lengths and thus improves molecular diffusion. This has been realized by synthesizing zeolite nanocrystals, by exfoliating layered zeolites, and by introducing mesopores in the microporous material through templating strategies or demetallation processes. But except for the exfoliation, none of these strategies has produced 'ultrathin' zeolites with thicknesses below 5 nm. Here we show that appropriately designed bifunctional surfactants can direct the formation of zeolite structures on the mesoporous and microporous length scales simultaneously and thus yield MFI (ZSM-5, one of the most important catalysts in the petrochemical industry) zeolite nanosheets that are only 2 nm thick, which corresponds to the b-axis dimension of a single MFI unit cell. The large number of acid sites on the external surface of these zeolites renders them highly active for the catalytic conversion of large organic molecules, and the reduced crystal thickness facilitates diffusion and thereby dramatically suppresses catalyst deactivation through coke deposition during methanol-to-gasoline conversion. We expect that our synthesis approach could be applied to other zeolites to improve their performance in a range of important catalytic applications.

  5. Transplatin enhances effect of cisplatin on both single DNA molecules and live tumor cells.

    Science.gov (United States)

    Liu, Yu-Ru; Ji, Chao; Zhang, Hong-Yan; Dou, Shuo-Xing; Xie, Ping; Wang, Wei-Chi; Wang, Peng-Ye

    2013-08-01

    Cisplatin is the main platinum antitumor drug applied in clinical settings. However, its trans isomer, transplatin, is known to have an ineffective antitumor activity. Despite intensive studies in this field, the structural and biophysical properties of DNA molecules reacting with these two platinum complexes have not been fully elucidated. In the present study, we observed that transplatin made efficient cross-linking of DNA in the vicinity of cisplatin adducts. High-resolution atomic force microscopy studies revealed that the transplatin-induced cross-linkings of nucleotides flanking cisplatin adducts were characterized by kinked-loop structures with rod-like shapes of nanometer scales (∼10-60nm). The results were further confirmed by denaturing gel electrophoresis and single-molecule experiment using magnetic tweezers. In vivo studies revealed that transplatin and cisplatin co-treatment could induce a considerable amount of kinked loops with smaller sizes (∼15nm) in cellular DNA. Furthermore, compared with cisplatin treatment alone, the co-treatment resulted in enhanced cytotoxicity, increased amount of interstrand cross-links, and cell lesions more reluctant to cellular repair system. The results of the present study provide a new clue for understanding the stepwise reactions of DNA with platinum drugs and might serve as a basis for the development of a new antitumor strategy. Copyright © 2013 Elsevier Inc. All rights reserved.

  6. A microfluidic device integrating plasmonic nanodevices for Raman spectroscopy analysis on trapped single living cells

    KAUST Repository

    Perozziello, Gerardo

    2013-11-01

    In this work we developed a microfluidic device integrating nanoplasmonic devices combined with fluidic trapping regions. The microfuidic traps allow to capture single cells in areas where plasmonic sensors are placed. In this way it is possible to perform Enhanced Raman analysis on the cell membranes. Moreover, by changing direction of the flux it is possible to change the orientation of the cell in the trap, so that it is possible to analyze different points of the membrane of the same cell. We shows an innovative procedure to fabricate and assembly the microfluidic device which combine photolithography, focused ion beam machining, and hybrid bonding between a polymer substrate and lid of Calcium fluoride. This procedure is compatible with the fabrication of the plasmonic sensors in close proximity of the microfluidic traps. Moreover, the use of Calcium fluoride as lid allows full compatibility with Raman measurements producing negligible Raman background signal and avoids Raman artifacts. Finally, we performed Raman analysis on cells to monitor their oxidative stress under particular non physiological conditions. © 2013 Elsevier B.V. All rights reserved.

  7. Religiosity of Muslim Adolescents from Single Parent Families Living in Government-Subsidised Settlement

    Directory of Open Access Journals (Sweden)

    Fariza, M. S.

    2015-12-01

    Full Text Available Adolescence is a phase of emotionl turmoil and stress. If not balanced with religious practice, it may cause adolescents to fall into moral and social problems such as breach of school discipline, delinquency, drug addiction and sexual permissiveness. Religiosity is one way for adolescents to deal with challenges in life thereby avoiding immoral activities. This is because religiosity may help to balance adolescent emotional, cognitive and social developments.The purpose of this study is to identify the behaviour and level of religiosity among 30 adolescents from single parents families who settle in government-subsidised area of residence, namely known as the Federal Land Development Authority (FELDA. The area is located in the state of Pahang, situated on the eastern part of Malaysia. These adolescents have been selected as research respondents. This research was conducted as a survey using questionnaire as the research instrument. It was constructed based on the guidelines of Islamic Practice Instrument (IPI (Fariza, 2012 and has already been verified in terms of its validity and reliability. The data analysis has adopted the method of descriptive statistics explained in table form. The research results have found that religiosity or religious behaviour that the adolescents most frequently abide by are staying away from gambling, having good thoughts and expectations of Allah, feeling awed and fear of Allah’s power (taqwa, staying away from fornication (zina and supplication (doa to Allah facing the direction of Qibla. The level of religiosity is medium for most adolescents. This factor explains why not many respondents are involved in moral and disciplinary problems at school. This research finds that generally, religiosity protects adolescents from being involved in immoral activities and other vices.

  8. Principle of single and multiple time interval analysis applicable to radioactive nuclides with half-lives of millisecond order

    International Nuclear Information System (INIS)

    Hashimoto, T.; Noguchi, M.; Washio, H.; Yamamoto, Y.; Uezu, Y.

    1992-01-01

    A delayed coincidence method has been developed for the selective extraction of radionuclides with half-lives of millisecond order using a personal computer. The time interval analysis is fundamentally based on the distinction of the time interval distribution between the correlated and random events, which arise from radioactive disintegration and/or background events within a certain fixed time. Theoretical functions of a time spectrum or a time distribution curve were dealt with from aspects of statistical treatments consisting of a single time interval analysis and a multiple one (STA and MTA). The theoretical equations obtained were used to simulate time spectra on the assumption of some ideal data and compared with the experimental results. Conclusively, the MTA method has been verified to be more suitable for the selective extraction of the objective correlated events from random or background events for the search of radioactive nuclides with millisecond life-times. (author) 11 refs.; 8 figs

  9. Artificial Formation and Tuning of Glycoprotein Networks on Live Cell Membranes: A Single-Molecule Tracking Study.

    Science.gov (United States)

    Möckl, Leonhard; Lindhorst, Thisbe K; Bräuchle, Christoph

    2016-03-16

    We present a method to artificially induce network formation of membrane glycoproteins and show the precise tuning of their interconnection on living cells. For this, membrane glycans are first metabolically labeled with azido sugars and then tagged with biotin by copper-free click chemistry. Finally, these biotin-tagged membrane proteins are interconnected with streptavidin (SA) to form an artificial protein network in analogy to a lectin-induced lattice. The degree of network formation can be controlled by the concentration of SA, its valency, and the concentration of biotin on membrane proteins. This was verified by investigation of the spatiotemporal dynamics of the SA-protein networks employing single-molecule tracking. It was also proven that this network formation strongly influences the biologically relevant process of endocytosis as it is known from natural lattices on the cell surface. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Localization of protein-protein interactions among three fluorescent proteins in a single living cell: three-color FRET microscopy

    Science.gov (United States)

    Sun, Yuansheng; Booker, Cynthia F.; Day, Richard N.; Periasamy, Ammasi

    2009-02-01

    Förster resonance energy transfer (FRET) methodology has been used for over 30 years to localize protein-protein interactions in living specimens. The cloning and modification of various visible fluorescent proteins (FPs) has generated a variety of new probes that can be used as FRET pairs to investigate the protein associations in living cells. However, the spectral cross-talk between FRET donor and acceptor channels has been a major limitation to FRET microscopy. Many investigators have developed different ways to eliminate the bleedthrough signals in the FRET channel for one donor and one acceptor. We developed a novel FRET microscopy method for studying interactions among three chromophores: three-color FRET microscopy. We generated a genetic construct that directly links the three FPs - monomeric teal FP (mTFP), Venus and tandem dimer Tomato (tdTomato), and demonstrated the occurrence of mutually dependent energy transfers among the three FPs. When expressed in cells and excited with the 458 nm laser line, the mTFP-Venus-tdTomato fusion proteins yielded parallel (mTFP to Venus and mTFP to tdTomato) and sequential (mTFP to Venus and then to tdTomato) energy transfer signals. To quantify the FRET signals in the three-FP system in a single living cell, we developed an algorithm to remove all the spectral cross-talk components and also to separate different FRET signals at a same emission channel using the laser scanning spectral imaging and linear unmixing techniques on the Zeiss510 META system. Our results were confirmed with fluorescence lifetime measurements and using acceptor photobleaching FRET microscopy.

  11. Convergence of lateral dynamic measurements in the plasma membrane of live cells from single particle tracking and STED-FCS

    Science.gov (United States)

    Lagerholm, B. Christoffer; Andrade, Débora M.; Clausen, Mathias P.; Eggeling, Christian

    2017-02-01

    Fluorescence correlation spectroscopy (FCS) in combination with the super-resolution imaging method STED (STED-FCS), and single-particle tracking (SPT) are able to directly probe the lateral dynamics of lipids and proteins in the plasma membrane of live cells at spatial scales much below the diffraction limit of conventional microscopy. However, a major disparity in interpretation of data from SPT and STED-FCS remains, namely the proposed existence of a very fast (unhindered) lateral diffusion coefficient, ⩾5 µm2 s-1, in the plasma membrane of live cells at very short length scales, ≈⩽ 100 nm, and time scales, ≈1-10 ms. This fast diffusion coefficient has been advocated in several high-speed SPT studies, for lipids and membrane proteins alike, but the equivalent has not been detected in STED-FCS measurements. Resolving this ambiguity is important because the assessment of membrane dynamics currently relies heavily on SPT for the determination of heterogeneous diffusion. A possible systematic error in this approach would thus have vast implications in this field. To address this, we have re-visited the analysis procedure for SPT data with an emphasis on the measurement errors and the effect that these errors have on the measurement outputs. We subsequently demonstrate that STED-FCS and SPT data, following careful consideration of the experimental errors of the SPT data, converge to a common interpretation which for the case of a diffusing phospholipid analogue in the plasma membrane of live mouse embryo fibroblasts results in an unhindered, intra-compartment, diffusion coefficient of  ≈0.7-1.0 µm2 s-1, and a compartment size of about 100-150 nm.

  12. Precision Optogenetic Tool for Selective Single- and Multiple-Cell Ablation in a Live Animal Model System.

    Science.gov (United States)

    Makhijani, Kalpana; To, Tsz-Leung; Ruiz-González, Rubén; Lafaye, Céline; Royant, Antoine; Shu, Xiaokun

    2017-01-19

    Cell ablation is a strategy to study cell lineage and function during development. Optogenetic methods are an important cell-ablation approach, and we have previously developed a mini singlet oxygen generator (miniSOG) tool that works in the living Caenorhabditis elegans. Here, we use directed evolution to generate miniSOG2, an improved tool for cell ablation via photogenerated reactive oxygen species. We apply miniSOG2 to a far more complex model animal system, Drosophila melanogaster, and demonstrate that it can be used to kill a single neuron in a Drosophila larva. In addition, miniSOG2 is able to photoablate a small group of cells in one of the larval wing imaginal discs, resulting in an adult with one incomplete and one normal wing. We expect miniSOG2 to be a useful optogenetic tool for precision cell ablation at a desired developmental time point in live animals, thus opening a new window into cell origin, fate and function, tissue regeneration, and developmental biology. Published by Elsevier Ltd.

  13. Under the Microscope: Single-Domain Antibodies for Live-Cell Imaging and Super-Resolution Microscopy

    Directory of Open Access Journals (Sweden)

    Bjoern Traenkle

    2017-08-01

    Full Text Available Single-domain antibodies (sdAbs have substantially expanded the possibilities of advanced cellular imaging such as live-cell or super-resolution microscopy to visualize cellular antigens and their dynamics. In addition to their unique properties including small size, high stability, and solubility in many environments, sdAbs can be efficiently functionalized according to the needs of the respective imaging approach. Genetically encoded intrabodies fused to fluorescent proteins (chromobodies have become versatile tools to study dynamics of endogenous proteins in living cells. Additionally, sdAbs conjugated to organic dyes were shown to label cellular structures with high density and minimal fluorophore displacement making them highly attractive probes for super-resolution microscopy. Here, we review recent advances of the chromobody technology to visualize localization and dynamics of cellular targets and the application of chromobody-based cell models for compound screening. Acknowledging the emerging importance of super-resolution microscopy in cell biology, we further discuss advantages and challenges of sdAbs for this technology.

  14. Working Mothers

    Science.gov (United States)

    ... children are harmed when their mothers work. A child’s development is influenced more by the emotional health of ... children for school, both socially and intellectually. The Importance of Quality Child Care Parents all wish for the best start ...

  15. Estimation of single-year-of-age counts of live births, fetal losses, abortions, and pregnant women for counties of Texas.

    Science.gov (United States)

    Singh, Bismark; Meyers, Lauren Ancel

    2017-05-08

    We provide a methodology for estimating counts of single-year-of-age live-births, fetal-losses, abortions, and pregnant women from aggregated age-group counts. As a case study, we estimate counts for the 254 counties of Texas for the year 2010. We use interpolation to estimate counts of live-births, fetal-losses, and abortions by women of each single-year-of-age for all Texas counties. We then use these counts to estimate the numbers of pregnant women for each single-year-of-age, which were previously available only in aggregate. To support public health policy and planning, we provide single-year-of-age estimates of live-births, fetal-losses, abortions, and pregnant women for all Texas counties in the year 2010, as well as the estimation method source code.

  16. Recent Developments in the Synthesis of Biomacromolecules and their Conjugates by Single Electron Transfer-Living Radical Polymerization.

    Science.gov (United States)

    Lligadas, Gerard; Grama, Silvia; Percec, Virgil

    2017-04-10

    Single electron transfer-living radical polymerization (SET-LRP) represents a robust and versatile tool for the synthesis of vinyl polymers with well-defined topology and chain end functionality. The crucial step in SET-LRP is the disproportionation of the Cu(I)X generated by activation with Cu(0) wire, powder, or nascent Cu(0) generated in situ into nascent, extremely reactive Cu(0) atoms and nanoparticles and Cu(II)X 2 . Nascent Cu(0) activates the initiator and dormant chains via a homogeneous or heterogeneous outer-sphere single-electron transfer mechanism (SET-LRP). SET-LRP provides an ultrafast polymerization of a plethora of monomers (e.g., (meth)-acrylates, (meth)-acrylamides, styrene, and vinyl chloride) including hydrophobic and water insoluble to hydrophilic and water soluble. Some advantageous features of SET-LRP are (i) the use of Cu(0) wire or powder as readily available catalysts under mild reaction conditions, (ii) their excellent control over molecular weight evolution and distribution as well as polymer chain ends, (iii) their high functional group tolerance allowing the polymerization of commercial-grade monomers, and (iv) the limited purification required for the resulting polymers. In this Perspective, we highlight the recent advancements of SET-LRP in the synthesis of biomacromolecules and of their conjugates.

  17. Study of Mothers' Anxieties Related to Their Children's Future

    Science.gov (United States)

    Ilgar, Sengul

    2015-01-01

    The purpose of this study is to study anxieties of mothers related to their children's future. Qualitative method was used in order to study anxieties of mothers from different socio-economic levels. Sample of the study participants are 129 mothers living in Istanbul. 32 of those mothers are from upper socio-economic level, 57, from middle…

  18. "My mother told me that I should not": a qualitative study exploring the restrictions placed on adolescent girls living with HIV in Zambia.

    Science.gov (United States)

    Mackworth-Young, Constance Rs; Bond, Virginia; Wringe, Alison; Konayuma, Katongo; Clay, Sue; Chiiya, Chipo; Chonta, Mutale; Sievwright, Kirsty; Stangl, Anne L

    2017-12-01

    Adolescent girls in sub-Saharan Africa are disproportionately affected by HIV due to a range of social and structural factors. As they transition to adulthood, they are recipients of increasing blame for HIV infection and 'improper' sex, as well as increasing scrutiny, restrictions and surveillance. This study used a qualitative and participatory approach to explore the messaging and restrictions imposed on adolescent girls living with HIV in Zambia. Thirty-four in-depth interviews and four participatory workshops were carried out with 24 adolescent girls aged 15 to 19 years old living with HIV in Lusaka, Zambia. Key themes explored included experiences living with HIV, finding out about HIV status, disclosure, experiences with antiretroviral treatment, and support needs. Data were organized, coded and analysed using a grounded theory approach to thematic analysis. This analysis uses data on participants' experiences of living with HIV and their interactions with their parents, guardians and healthcare providers. Family and healthcare providers, partly in a quest to protect both the health of adolescent girls living with HIV and also to protect them from blaming discourse, imposed restrictions on their behaviour around three main topics: don't disclose your HIV status, don't have sex, and don't miss your medicines. These restrictions were often delivered using tactics of fear, and usually disconnected from other options. Participants responded to these messages in several ways, including internalizing the messages, changing their behaviour either to comply with or resist the restrictions, by remaining silent and anxious when restrictions were broken, and developing concerns around their own health and sexual and reproductive aspirations. Participants also sometimes experiencing stigma when restrictions could not be maintained. Restrictive messages were delivered to adolescent girls living with HIV through the broader social discourses of stigma, religion, and

  19. PCB Concentrations and Dioxin-like Activity in Blood Samples from Danish School Children and Their Mothers living in Urban and Rural Areas

    DEFF Research Database (Denmark)

    Mørck, Thit A; Erdmann, Simon E; Long, Manhai

    2014-01-01

    Human exposure to persistent organic pollutants (POPs) is of major concern due to a diversity of adverse effects from prolonged exposure and bioaccumulation. Manufacturing of polychlorinated biphenyls (PCBs), a subgroup of POPs, has been prohibited for many decades; however, human exposure still...... occurs due to the persistent nature of the chemicals. The concentrations of the dioxin-like PCB congeners 105, 118 and 156 and the non-dioxin-like PCB congeners 28, 52, 101, 138, 153 and 180, p,p'-DDE, p,p'-DDT, o,p'-DDE, o,p'-DDT, HCB and β-HCH as well as the dioxin-like activity using the Ah......R transactivity assay were analysed in blood samples from Danish schoolchildren and their mothers in the European framework of the DEMOCOPHES/COPHES projects. The participants were selected from an urban and a rural area, respectively. The PCB concentrations and the AhR-TEQ (TCDD toxic equivalent) were...

  20. Etnoteorias parentais: um estudo com mães residentes no interior e na capital de Santa Catarina Parental ethnotheories: a study with mothers living in the capital and countryside of Santa Catarina State

    Directory of Open Access Journals (Sweden)

    Samira Mafioletti Macarini

    2010-04-01

    Full Text Available O estudo teve como objetivo investigar etnoteorias parentais em mães residentes na capital e interior de Santa Catarina. Foram aplicadas escalas de crenças sobre práticas de cuidado, metas de socialização e alocentrismo familiar em 50 mães de cada contexto cultural. Os resultados na capital indicaram predomínio de elementos autônomo-relacionais em práticas e alocentrismo familiar, enquanto que em termos de metas, as mães valorizaram a autonomia da criança. No interior, no que se refere a metas de socialização e alocentrismo familiar, foram identificados aspectos relacionais. Contudo, em termos de crenças sobre práticas de criação, constatou-se maior valorização da autonomia. Concluiu-se que ambos os contextos caracterizam-se por um modelo de self autônomo-relacional, embora com diferentes ênfases de acordo com a dimensão investigada.The aim of this study was to investigate parental ethnotheories of mothers living in the capital city and in the countryside of Santa Catarina State. In each cultural environment, fifty mothers were interviewed based on scales that evaluated their beliefs about childrearing practices, socialization goals and degree of familism. Results from the capital demonstrated a predominance of autonomous-related elements for practices and familism, whereas in terms of socialization goals, mothers valued the autonomy of the child. In the countryside, relational aspects were identified for socialization goals and familism. However, for beliefs about childrearing practices it has been noted emphasis on autonomy. The conclusion was that both contexts are characterized by an autonomous-related self, although with different emphases according to the dimension investigated.

  1. The relationship between raising a child with a disability and the mental health of mothers compared to raising a child without disability in japan

    Directory of Open Access Journals (Sweden)

    Yui Yamaoka

    2016-12-01

    Full Text Available Objective: Previous studies conducted in Japan targeted only mothers who cared for children with disabilities, and lacked reference subjects, such as mothers of children without disabilities. The aim of this study was to examine the association between raising one or two children with a disability and maternal psychological distress compared to mothers of children without a disability, and to assess differences among partnered mothers living with grandparent(s, partnered mothers without grandparent(s, and single mothers. Methods: This study utilized data from the Comprehensive Survey of Living Conditions (CSLC in 2010. We merged the data of the children (aged six and over, mothers, and fathers. This study obtained 33,739 study subjects as a triad of a child (33,110 children without disabilities and 629 children with disabilities, mother, and father. The Japanese version of Kessler 6 (K6 was used to assess the psychological distress of mothers. Multivariate logistic regression was performed to assess the independent association of a child with a disability on maternal psychological distress after controlling for the basic characteristics of the children, mothers, and households. Results: This study reported that raising one or two children with disabilities was significantly related to maternal psychological distress (odds ratio: 1.72 for one child, 2.85 for two children compared to mothers of children without disability. After stratifying the analyses by family structure, significant associations remained among mothers in two-parent families but not for mothers in three-generation families and single mothers due to a small number of children with disabilities in these families. Conclusions: This study reported the significant association between raising a child with a disability and maternal psychological distress in comparison to mothers of children without disabilities. Attention should be paid to not only single mothers, but also partnered

  2. A seven-year investigation of marital expectations and marriage among urban, low-income, African American adolescent mothers.

    Science.gov (United States)

    Oberlander, Sarah E; Agostini, Wendy R Miller; Houston, Avril Melissa; Black, Maureen M

    2010-02-01

    Welfare reform has targeted marriage promotion among low-income women. This study explores patterns of marital expectations and marriage among 181 urban, low-income, African American adolescent mothers and their mothers. Using PROC TRAJ to analyze developmental trajectories of adolescent mother-grandmother relationship quality over 24 months, we categorized relationships as either high or low support. We examined the effects of intergenerational marriage models and adolescent mother-grandmother relationship quality on marital expectations and marriage over the first 7 years postpartum. At 24 months, half (52%) of adolescent mothers expected to marry, but marital expectations did not predict marriage. Marital expectations were associated with concurrent involvement in a romantic relationship, not intergenerational marriage models or a supportive adolescent mother-grandmother relationship. After 7 years, 14% of adolescent mothers were married. Married mothers lived in families characterized by the joint effects of intergenerational marriage models and supportive adolescent mother-grandmother relationships. They were older and had more children than did single mothers, suggesting that they were in a family formation phase of life. Policies that promote the education and employment opportunities necessary to support a family are needed.

  3. Transcription-factor-mediated DNA looping probed by high-resolution, single-molecule imaging in live E. coli cells.

    Directory of Open Access Journals (Sweden)

    Zach Hensel

    Full Text Available DNA looping mediated by transcription factors plays critical roles in prokaryotic gene regulation. The "genetic switch" of bacteriophage λ determines whether a prophage stays incorporated in the E. coli chromosome or enters the lytic cycle of phage propagation and cell lysis. Past studies have shown that long-range DNA interactions between the operator sequences O(R and O(L (separated by 2.3 kb, mediated by the λ repressor CI (accession number P03034, play key roles in regulating the λ switch. In vitro, it was demonstrated that DNA segments harboring the operator sequences formed loops in the presence of CI, but CI-mediated DNA looping has not been directly visualized in vivo, hindering a deep understanding of the corresponding dynamics in realistic cellular environments. We report a high-resolution, single-molecule imaging method to probe CI-mediated DNA looping in live E. coli cells. We labeled two DNA loci with differently colored fluorescent fusion proteins and tracked their separations in real time with ∼40 nm accuracy, enabling the first direct analysis of transcription-factor-mediated DNA looping in live cells. Combining looping measurements with measurements of CI expression levels in different operator mutants, we show quantitatively that DNA looping activates transcription and enhances repression. Further, we estimated the upper bound of the rate of conformational change from the unlooped to the looped state, and discuss how chromosome compaction may impact looping kinetics. Our results provide insights into transcription-factor-mediated DNA looping in a variety of operator and CI mutant backgrounds in vivo, and our methodology can be applied to a broad range of questions regarding chromosome conformations in prokaryotes and higher organisms.

  4. Peptide-coated semiconductor quantum dots and their applications in biological imaging of single molecules in live cells and organisms

    Science.gov (United States)

    Pinaud, Fabien Florent

    2007-12-01

    A new surface chemistry has been developed for the solubilization and biofunctionalization of inorganic semiconductor nanocrystals fluorescent probes, also known as quantum dots. This chemistry is based on the surface coating of quantum dots with custom-designed polycysteine peptides and yields water-soluble, small, monodispersed and colloidally stable probes that remain bright and photostable in complex biological milieus. This peptide coating strategy was successfully tested on several types of core and core-shell quantum dots emitting from the visible (e.g. CdSe/ZnS) to the NIR spectrum range (e.g. CdTe/CdSe/ZnS). By taking advantage of the versatile physico-chemical properties of peptides, a peptide "toolkit" was designed and employed to impart several biological functions to individual quantum dots and control their biochemical activity at the nanometer scale. These biofunctionalized peptide-coated quantum dots were exploited in very diverse biological applications. Near-infrared emitting quantum dot probes were engineered with optimized blood circulation and biodistribution properties for in vivo animal imaging. Visible emitting quantum dots were used for single molecule tracking of raft-associated GPI-anchored proteins in live cells. This last application revealed the presence of discrete and non-caveolar lipid microdomains capable of impeding free lateral diffusions in the plasma membrane of Hela cells. Imaging and tracking of peptide-coated quantum dots provided the first direct evidence that microdomains having the composition and behavior expected for lipid rafts can induce molecular compartmentalization in the membrane of living cells.

  5. [Unwed mothers].

    Science.gov (United States)

    Kofayat, M

    1978-01-01

    The problem of unwed mothers is becoming a crucial one in most African countries. The great majority of unmarried adolescents are forced to quit school, and they very often become prostitutes. The problem has several causes; exodus from rural areas, rapid change of attitudes toward sexuality, lack of sex education, sudden disappearance of certain cultural traditions. It would be necessary to create centers where unwed mothers can stay, learn a job, or continue their studies, and, especially, it would be advisable to promote the insertion of young people in traditional jobs in their own villages.

  6. Optical control and study of biological processes at the single-cell level in a live organism

    Science.gov (United States)

    Feng, Zhiping; Zhang, Weiting; Xu, Jianmin; Gauron, Carole; Ducos, Bertrand; Vriz, Sophie; Volovitch, Michel; Jullien, Ludovic; Weiss, Shimon; Bensimon, David

    2013-07-01

    Living organisms are made of cells that are capable of responding to external signals by modifying their internal state and subsequently their external environment. Revealing and understanding the spatio-temporal dynamics of these complex interaction networks is the subject of a field known as systems biology. To investigate these interactions (a necessary step before understanding or modelling them) one needs to develop means to control or interfere spatially and temporally with these processes and to monitor their response on a fast timescale (single-cell resolution. In 2012, an EMBO workshop on ‘single-cell physiology’ (organized by some of us) was held in Paris to discuss those issues in the light of recent developments that allow for precise spatio-temporal perturbations and observations. This review will be largely based on the investigations reported there. We will first present a non-exhaustive list of examples of cellular interactions and developmental pathways that could benefit from these new approaches. We will review some of the novel tools that have been developed for the observation of cellular activity and then discuss the recent breakthroughs in optical super-resolution microscopy that allow for optical observations beyond the diffraction limit. We will review the various means to photo-control the activity of biomolecules, which allow for local perturbations of physiological processes. We will end up this review with a report on the current status of optogenetics: the use of photo-sensitive DNA-encoded proteins as sensitive reporters and efficient actuators to perturb and monitor physiological processes.

  7. Poly(N-isopropylacrylamide) brushes grafted from cellulose nanocrystals via surface-initiated single-electron transfer living radical polymerization.

    Science.gov (United States)

    Zoppe, Justin O; Habibi, Youssef; Rojas, Orlando J; Venditti, Richard A; Johansson, Leena-Sisko; Efimenko, Kirill; Osterberg, Monika; Laine, Janne

    2010-10-11

    Cellulose nanocrystals (CNCs) or nanowhiskers produced from sulfuric acid hydrolysis of ramie fibers were used as substrates for surface chemical functionalization with thermoresponsive macromolecules. The CNCs were grafted with poly(N-isopropylacrylamide) brushes via surface-initiated single-electron transfer living radical polymerization (SI-SET-LRP) under various conditions at room temperature. The grafting process was confirmed via Fourier transform IR spectroscopy and X-ray photoelectron spectroscopy and the different molecular masses of the grafts were quantified and found to depend on the initiator and monomer concentrations used. No observable damage occurred to the CNCs after grafting, as determined by X-ray diffraction. Size exclusion chromatography analyses of polymer chains cleaved from the cellulose nanocrystals indicated that a higher degree of polymerization was achieved by increasing initiator or monomer loading, most likely caused by local heterogeneities yielding higher rates of polymerization. It is expected that suspension stability, interfacial interactions, friction, and other properties of grafted CNCs can be controlled by changes in temperature and provide a unique platform for further development of stimuli-responsive nanomaterials.

  8. Effect of a single session of aerobic walking exercise on arterial pressure in community-living elderly individuals.

    Science.gov (United States)

    Lima, Leandra G; Moriguti, Júlio C; Ferriolli, Eduardo; Lima, Nereida K C

    2012-04-01

    Several studies have demonstrated that one exercise session (ES) on a cycloergometer or ergometric treadmill causes a reduction in blood pressure (BP). However, there are few similar studies on walking, which is the exercise modality most available to the elderly. We investigated the immediate and 24-h effects of walking on BP in independent, community-living elderly individuals. Volunteers participated in a single ES and resting control session (CS). Before and after each session, BP was measured by auscultatory and oscillometric methods. After each session, 24-h ambulatory blood pressure monitoring was conducted. An accelerometer was installed 48 h before the sessions and left in place for 5 days. The mean volunteer age was 67.7±3.5 years; 11 were hypertensive patients under treatment, and 12 were normotensive. In the total sample, there were immediate 14mm Hg and 12 mm Hg reductions in systolic BP (SBP) after the ES according to the auscultatory and oscillometric methods, respectively. Diastolic BP (DBP) was reduced by 4 mm Hg after the ES according to both methods. SBP during wakefulness and sleep and DBP during wakefulness were lower after the ES than after the CS (Psleep were determined individually (variable-time pattern) using data from the activity monitors and provided by the volunteers. The variable-time pattern was more effective in detecting reductions in BP than the fixed-time pattern.

  9. A micro-Raman study of live, single red blood cells (RBCs treated with AgNO3 nanoparticles.

    Directory of Open Access Journals (Sweden)

    Aseefhali Bankapur

    Full Text Available Silver nanoparticles (Ag NPs are known to exhibit broad antimicrobial activity. However, such activity continues to raise concerns in the context of the interaction of such NPs with biomolecules. In a physiological environment NPs interact with individual biological cells either by penetrating through the cell membrane or by adhering to the membrane. We have explored the interaction of Ag NPs with single optically-trapped, live erythrocytes (red blood cells, RBCs using Raman Tweezers spectroscopy. Our experiments reveal that Ag NPs induce modifications within an RBC that appear to be irreversible. In particular we are able to identify that the heme conformation in an RBC transforms from the usual R-state (oxy-state to the T-state (deoxy-state. We rationalize our observations by proposing a model for the nanoparticle cytotoxicity pathway when the NP size is larger than the membrane pore size. We propose that the interaction of Ag NPs with the cell surface induces damage brought about by alteration of intracellular pH caused by the blockage of the cell membrane transport.

  10. A New Genetically Encoded Single-Chain Biosensor for Cdc42 Based on FRET, Useful for Live-Cell Imaging

    Science.gov (United States)

    Cox, Dianne; Hodgson, Louis

    2014-01-01

    Cdc42 is critical in a myriad of cellular morphogenic processes, requiring precisely regulated activation dynamics to affect specific cellular events. To facilitate direct observations of Cdc42 activation in live cells, we developed and validated a new biosensor of Cdc42 activation. The biosensor is genetically encoded, of single-chain design and capable of correctly localizing to membrane compartments as well as interacting with its upstream regulators including the guanine nucleotide dissociation inhibitor. We characterized this new biosensor in motile mouse embryonic fibroblasts and observed robust activation dynamics at leading edge protrusions, similar to those previously observed for endogenous Cdc42 using the organic dye-based biosensor system. We then extended our validations and observations of Cdc42 activity to macrophages, and show that this new biosensor is able to detect differential activation patterns during phagocytosis and cytokine stimulation. Furthermore, we observe for the first time, a highly transient and localized activation of Cdc42 during podosome formation in macrophages, which was previously hypothesized but never directly visualized. PMID:24798463

  11. Long-Lived Charge Separation at Heterojunctions between Semiconducting Single-Walled Carbon Nanotubes and Perylene Diimide Electron Acceptors

    Energy Technology Data Exchange (ETDEWEB)

    Kang, Hyun Suk [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Arias, Dylan H [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Blackburn, Jeffrey L [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Sisto, Thomas J. [Columbia University; Peurifoy, Samuel [Columbia University; Zhang, Boyuan [Columbia University; Nuckolls, Colin [Columbia University

    2018-04-13

    Nonfullerene electron acceptors have facilitated a recent surge in the efficiencies of organic solar cells, although fundamental studies of the nature of exciton dissociation at interfaces with nonfullerene electron acceptors are still relatively sparse. Semiconducting single-walled carbon nanotubes (s-SWCNTs), unique one-dimensional electron donors with molecule-like absorption and highly mobile charges, provide a model system for studying interfacial exciton dissociation. Here, we investigate excited-state photodynamics at the heterojunction between (6,5) s-SWCNTs and two perylene diimide (PDI)-based electron acceptors. Each of the PDI-based acceptors, hPDI2-pyr-hPDI2 and Trip-hPDI2, is deposited onto (6,5) s-SWCNT films to form a heterojunction bilayer. Transient absorption measurements demonstrate that photoinduced hole/electron transfer occurs at the photoexcited bilayer interfaces, producing long-lived separated charges with lifetimes exceeding 1.0 us. Both exciton dissociation and charge recombination occur more slowly for the hPDI2-pyr-hPDI2 bilayer than for the Trip-hPDI2 bilayer. To explain such differences, we discuss the potential roles of the thermodynamic charge transfer driving force available at each interface and the different molecular structure and intermolecular interactions of PDI-based acceptors. Detailed photophysical analysis of these model systems can develop the fundamental understanding of exciton dissociation between organic electron donors and nonfullerene acceptors, which has not been systematically studied.

  12. The relationship between raising a child with a disability and the mental health of mothers compared to raising a child without disability in japan.

    Science.gov (United States)

    Yamaoka, Yui; Tamiya, Nanako; Izumida, Nobuyuki; Kawamura, Akira; Takahashi, Hideto; Noguchi, Haruko

    2016-12-01

    Previous studies conducted in Japan targeted only mothers who cared for children with disabilities, and lacked reference subjects, such as mothers of children without disabilities. The aim of this study was to examine the association between raising one or two children with a disability and maternal psychological distress compared to mothers of children without a disability, and to assess differences among partnered mothers living with grandparent(s), partnered mothers without grandparent(s), and single mothers. This study utilized data from the Comprehensive Survey of Living Conditions (CSLC) in 2010. We merged the data of the children (aged six and over), mothers, and fathers. This study obtained 33,739 study subjects as a triad of a child (33,110 children without disabilities and 629 children with disabilities), mother, and father. The Japanese version of Kessler 6 (K6) was used to assess the psychological distress of mothers. Multivariate logistic regression was performed to assess the independent association of a child with a disability on maternal psychological distress after controlling for the basic characteristics of the children, mothers, and households. This study reported that raising one or two children with disabilities was significantly related to maternal psychological distress (odds ratio: 1.72 for one child, 2.85 for two children) compared to mothers of children without disability. After stratifying the analyses by family structure, significant associations remained among mothers in two-parent families but not for mothers in three-generation families and single mothers due to a small number of children with disabilities in these families. This study reported the significant association between raising a child with a disability and maternal psychological distress in comparison to mothers of children without disabilities. Attention should be paid to not only single mothers, but also partnered mothers in two-parent families who have a child with a

  13. Decision making for mothers with cancer: maintaining the mother-child bond.

    Science.gov (United States)

    Campbell-Enns, Heather J; Woodgate, Roberta L

    2013-06-01

    The objective of this study was to explore the process of decision-making in mothers with cancer when they are mothering young children. The purpose of this article is to describe the core category that emerged from the study as well as the conditions precipitating decision making and the consequences of decision making for mothers with cancer. A qualitative methodology based on the tenets of constructivist grounded theory was used to conduct ten interviews with eight mothers with cancer. Data analysis revealed the core category, the conditions of the decision situation as well as the consequences of decision making. The core category was the meaning that mothers made of decisions, specifically that each decision was made to maintain the mother-child bond. The conditions of the mothers' lives influenced the meaning mothers assigned to decisions. The consequences of decision making were displayed by these mothers through coping strategies to facilitate maintaining the mother-child bond in times of distress. The conditions of the mothers' lives created a context in which mothers made meaning of decisions. Mothers aimed to maintain their bonds with their children in the decision making process and used various coping strategies as a consequence to distress from decisional situations. The results have implications for future decision making research in cancer care. Copyright © 2012 Elsevier Ltd. All rights reserved.

  14. Faith in action: serving single moms.

    Science.gov (United States)

    Briggs, Crista L; Lovan, Sherry; Cornell, Audrey

    2011-01-01

    In 2009, female-headed households represented 29.9% (4.4 million) of all U.S. families, with many living below the poverty line. A church in Bowling Green, Kentucky, began a Single Mothers' Oil Change service, incorporating health screening by nurses using Pender's Health Promotion Model. Nurses are equipped to identify health barriers and offer health promotion guidance and social support to single, low-income women.

  15. Live Attenuated Tetravalent Dengue Virus Host Range Vaccine Is Immunogenic in African Green Monkeys following a Single Vaccination

    Science.gov (United States)

    Smith, Katherine M.; Piper, Amanda; Huitt, Emerson; Spears, Carla J.; Quiles, Michelle; Ribeiro, Mariana; Thomas, Malcolm E.; Brown, Dennis T.; Hernandez, Raquel

    2014-01-01

    ABSTRACT The causative agent of dengue fever, dengue virus (DENV), is transmitted by mosquitoes, and as distribution of these insects has expanded, so has dengue-related disease. DENV is a member of the Flaviviridae family and has 4 distinct serotypes (DENV-1, -2, -3, and -4). No lasting cross protection is afforded to heterologous serotypes following infection by any one of the individual serotypes. The presence of nonneutralizing antibodies to one serotype can facilitate the occurrence of more-severe dengue hemorrhagic fever through immune enhancement upon infection with a second serotype. For this reason, the development of a safe, tetravalent vaccine to produce a balanced immune response to all four serotypes is critical. We have developed a novel approach to produce safe and effective live-attenuated vaccines for DENV and other insect-borne viruses. Host range (HR) mutants of each DENV serotype were created by truncating transmembrane domain 1 of the E protein and selecting for strains of DENV that replicated well in insect cells but not mammalian cells. These vaccine strains were tested for immunogenicity in African green monkeys (AGMs). No vaccine-related adverse events occurred. The vaccine strains were confirmed to be attenuated in vivo by infectious center assay (ICA). Analysis by 50% plaque reduction neutralization test (PRNT50) established that by day 62 postvaccination, 100% of animals seroconverted to DENV-1, -2, -3, and -4. Additionally, the DENV HR tetravalent vaccine (HR-Tet) showed a tetravalent anamnestic immune response in 100% (16/16) of AGMs after challenge with wild-type (WT) DENV strains. IMPORTANCE We have generated a live attenuated viral (LAV) vaccine capable of eliciting a strong immune response in African green monkeys (AGMs) in a single dose. This vaccine is delivered by injecting one of four attenuated serotypes into each limb of the animal. 100% of animals given the vaccine generated antibodies against all 4 serotypes, and this

  16. Live attenuated tetravalent dengue virus host range vaccine is immunogenic in African green monkeys following a single vaccination.

    Science.gov (United States)

    Briggs, Caitlin M; Smith, Katherine M; Piper, Amanda; Huitt, Emerson; Spears, Carla J; Quiles, Michelle; Ribeiro, Mariana; Thomas, Malcolm E; Brown, Dennis T; Hernandez, Raquel

    2014-06-01

    The causative agent of dengue fever, dengue virus (DENV), is transmitted by mosquitoes, and as distribution of these insects has expanded, so has dengue-related disease. DENV is a member of the Flaviviridae family and has 4 distinct serotypes (DENV-1, -2, -3, and -4). No lasting cross protection is afforded to heterologous serotypes following infection by any one of the individual serotypes. The presence of nonneutralizing antibodies to one serotype can facilitate the occurrence of more-severe dengue hemorrhagic fever through immune enhancement upon infection with a second serotype. For this reason, the development of a safe, tetravalent vaccine to produce a balanced immune response to all four serotypes is critical. We have developed a novel approach to produce safe and effective live-attenuated vaccines for DENV and other insect-borne viruses. Host range (HR) mutants of each DENV serotype were created by truncating transmembrane domain 1 of the E protein and selecting for strains of DENV that replicated well in insect cells but not mammalian cells. These vaccine strains were tested for immunogenicity in African green monkeys (AGMs). No vaccine-related adverse events occurred. The vaccine strains were confirmed to be attenuated in vivo by infectious center assay (ICA). Analysis by 50% plaque reduction neutralization test (PRNT50) established that by day 62 postvaccination, 100% of animals seroconverted to DENV-1, -2, -3, and -4. Additionally, the DENV HR tetravalent vaccine (HR-Tet) showed a tetravalent anamnestic immune response in 100% (16/16) of AGMs after challenge with wild-type (WT) DENV strains. We have generated a live attenuated viral (LAV) vaccine capable of eliciting a strong immune response in African green monkeys (AGMs) in a single dose. This vaccine is delivered by injecting one of four attenuated serotypes into each limb of the animal. 100% of animals given the vaccine generated antibodies against all 4 serotypes, and this response was found to be

  17. A study of the diffusion dynamics and concentration distribution of gold nanospheres (GNSs) without fluorescent labeling inside live cells using fluorescence single particle spectroscopy.

    Science.gov (United States)

    Liu, Fangchao; Dong, Chaoqing; Ren, Jicun

    2018-03-15

    Colloidal gold nanospheres (GNSs) have become important nanomaterials in biomedical applications due to their special optical properties, good chemical stability, and biocompatibility. However, measuring the diffusion coefficients or concentration distribution of GNSs within live cells accurately without any extra fluorescent labeling in situ has still not been resolved. In this work, a single particle method is developed to study the concentration distribution of folic acid-modified GNSs (FA-GNSs) internalized via folate receptors, and investigates their diffusion dynamics within live cells using single particle fluorescence correlation spectroscopy (FCS). We optimized the experimental conditions and verified the feasibility of 30 nm GNSs without extra fluorescence labeling being used for single particle detection inside live cells. Then, the FCS characterization strategy was used to measure the concentration and diffusion coefficient distributions of GNSs inside live cells and the obtained results were basically in agreement with those obtained by TEM. The results demonstrate that our strategy is characterized as an in situ, nondestructive, rapid and dynamic method for the assay of live cells, and it may be widely used in the further design of GNP-based drug delivery and therapeutics.

  18. Strategic planning for saving the lives of mothers, newborns and children and preventing stillbirths in KwaZulu-Natal province South Africa: modelling using the Lives Saved Tool (LiST).

    Science.gov (United States)

    McGee, Shelley-Ann; Chola, Lumbwe; Tugendhaft, Aviva; Mubaiwa, Victoria; Moran, Neil; McKerrow, Neil; Kamugisha, Leonard; Hofman, Karen

    2016-01-19

    KwaZulu-Natal province in South Africa has the largest population of children under the age of five and experiences the highest number of child births per annum in the country. Its population has also been ravaged by the dual epidemics of HIV and TB and it has struggled to meet targets for maternal and child mortality. In South Africa's federal system, provinces have decision-making power on the prioritization and allocation of resources within their jurisdiction. As part of strategic planning for 2015-2019, KwaZulu-Natal provincial authorities requested an assessment of current mortality levels in the province and identification and costing of priority interventions for saving additional maternal, newborn and child lives, as well as preventing stillbirths in the province. The Lives Saved Tool (LiST) was used to determine the set of interventions, which could save the most additional maternal and child lives and prevent stillbirths from 2015-2019, and the costs of these. The impact of family planning was assessed using two scenarios by increasing baseline coverage of modern contraception by 0.5 percentage points or 1 percentage point per annum. A total of 7,043 additional child and 297 additional maternal lives could be saved, and 2,000 stillbirths could be prevented over five years. Seventeen interventions account for 75% of additional lives saved. Increasing family planning contributes to a further reduction of up to 137 maternal and 3,168 child deaths. The set of priority interventions scaled up to achievable levels, with no increase in contraception would require an additional US$91 million over five years or US$1.72 per capita population per year. By increasing contraceptive prevalence by one percentage point per year, overall costs to scale up to achievable coverage package, decrease by US$24 million over five years. Focused attention on a set of key interventions could have a significant impact on averting stillbirths and maternal and neonatal mortality in

  19. Birth Weight, Apgar Scores, Labor and Delivery Complications and Prenatal Characteristics of Southeast Asian Adolescents and Older Mothers.

    Science.gov (United States)

    Swenson, Ingrid; And Others

    1986-01-01

    Examined 337 adolescents and 876 older mothers who delivered live-born, single infants between 1980-1982. Absence of alcohol and tobacco consumption among Southeast Asian subjects may have contributed to generally favorable Apgar scores, length of gestation, and birth weights. High frequencies of alcohol and tobacco consumption among White…

  20. 'It is what it is': mothers' experiences of providing bladder and bowel care to their daughters living with life-limiting conditions.

    Science.gov (United States)

    Combes, Sarah; Woodward, Sue; Norton, Christine

    2017-12-02

    Informal carers (carers) support palliative patients to live at home. Most palliative patients require bladder and bowel care (B&BC) at some point, but there is limited evidence about carers providing B&BC and how best to support them. To explore carers' experiences of providing B&BC to life-limited young adults. Interpretative phenomenological analysis of five interviews with purposively selected carers. One overarching theme, 'It is what it is', and three superordinate themes ('Whatever my daughter needs', 'Mum knows best', and 'Coping with caring') emerged. While B&BC could be challenging at times, it was not a major concern. Instead it engendered closeness and opportunities for carers to provide better care than they felt professionals could. This study informs how professionals should understand the support carers offer. Professionals should ensure that carer/patient dyads who wish to manage B&BC are supported to do so, and that their support needs are regularly assessed.

  1. Polymerization Induced Self-Assembly of Alginate Based Amphiphilic Graft Copolymers Synthesized by Single Electron Transfer Living Radical Polymerization.

    Science.gov (United States)

    Kapishon, Vitaliy; Whitney, Ralph A; Champagne, Pascale; Cunningham, Michael F; Neufeld, Ronald J

    2015-07-13

    Alginate-based amphiphilic graft copolymers were synthesized by single electron transfer living radical polymerization (SET-LRP), forming stable micelles during polymerization induced self-assembly (PISA). First, alginate macroinitiator was prepared by partial depolymerization of native alginate, solubility modification and attachment of initiator. Depolymerized low molecular weight alginate (∼12 000 g/mol) was modified with tetrabutylammonium, enabling miscibility in anhydrous organic solvents, followed by initiator attachment via esterification yielding a macroinitiator with a degree of substitution of 0.02, or 1-2 initiator groups per alginate chain. Then, methyl methacrylate was polymerized from the alginate macroinitiator in mixtures of water and methanol, forming poly(methyl methacrylate) grafts, prior to self-assembly, of ∼75 000 g/mol and polydispersity of 1.2. PISA of the amphiphilic graft-copolymer resulted in the formation of micelles with diameters of 50-300 nm characterized by light scattering and electron microscopy. As the first reported case of LRP from alginate, this work introduces a synthetic route to a preparation of alginate-based hybrid polymers with a precise macromolecular architecture and desired functionalities. The intended application is the preparation of micelles for drug delivery; however, LRP from alginate can also be applied in the field of biomaterials to the improvement of alginate-based hydrogel systems such as nano- and microhydrogel particles, islet encapsulation materials, hydrogel implants, and topical applications. Such modified alginates can also improve the function and application of native alginates in food and agricultural applications.

  2. Breastfeeding Knowledge and Practices among Mothers of Children under 2 Years of Age Living in a Military Barrack in Southwest Nigeria

    Directory of Open Access Journals (Sweden)

    Modupe Rebekah Akinyinka, MBBS, MPH, FMCPH

    2016-02-01

    Full Text Available Background: Human milk is uniquely superior as a source of nutrition for infants, and breastfeeding has many benefits. This study determined the breastfeeding knowledge and practices of women who have children aged 0-2 years living in a Naval Barracks. Methods: This descriptive cross sectional study was carried out among 220 women in a Naval Barracks selected using systematic random sampling method. Pre-tested questionnaires were administered by trained interviewers, and data was analyzed using Epi info 2000 and Statistical Package for Social Sciences version 19. Results: There was generally fair knowledge about breastfeeding among the women. Most of the respondents (97.3% had ever breastfed their babies, 56.5% of them initiated breastfeeding within an hour of delivery, 24.1% admitted that they gave pre-lacteal feeds, 74.1% practiced exclusive breastfeeding for a mean period of 4.98 months and 30.7% engaged in bottle-feeding. Several factors were significantly associated with breastfeeding practices. Conclusions: Breastfeeding practices varied among the respondents despite the fair knowledge. Global Health Implications: This study reveals the need to educate women and communities worldwide particularly in low-income countries about good breastfeeding practices. Targeting these women will help to improve maternal and child health.

  3. Saving Mothers' Lives: Reviewing maternal deaths to make motherhood safer: 2006-2008. The Eighth Report of the Confidential Enquiries into Maternal Deaths in the United Kingdom.

    LENUS (Irish Health Repository)

    Cantwell, Roch

    2011-03-01

    In the triennium 2006-2008, 261 women in the UK died directly or indirectly related to pregnancy. The overall maternal mortality rate was 11.39 per 100,000 maternities. Direct deaths decreased from 6.24 per 100,000 maternities in 2003-2005 to 4.67 per 100,000 maternities in 2006–2008 (p = 0.02). This decline is predominantly due to the reduction in deaths from thromboembolism and, to a lesser extent, haemorrhage. For the first time there has been a reduction in the inequalities gap, with a significant decrease in maternal mortality rates among those living in the most deprived areas and those in the lowest socio-economic group. Despite a decline in the overall UK maternal mortality rate, there has been an increase in deaths related to genital tract sepsis, particularly from community acquired Group A streptococcal disease. The mortality rate related to sepsis increased from 0.85 deaths per 100,000 maternities in 2003-2005 to 1.13 deaths in 2006-2008, and sepsis is now the most common cause of Direct maternal death. Cardiac disease is the most common cause of Indirect death; the Indirect maternal mortality rate has not changed significantly since 2003-2005. This Confidential Enquiry identified substandard care in 70% of Direct deaths and 55% of Indirect deaths. Many of the identified avoidable factors remain the same as those identified in previous Enquiries. Recommendations for improving care have been developed and are highlighted in this report. Implementing the Top ten recommendations should be prioritised in order to ensure the overall UK maternal mortality rate continues to decline.

  4. Note: Multi-confocal fluorescence correlation spectroscopy in living cells using a complementary metal oxide semiconductor-single photon avalanche diode array

    Science.gov (United States)

    Kloster-Landsberg, M.; Tyndall, D.; Wang, I.; Walker, R.; Richardson, J.; Henderson, R.; Delon, A.

    2013-07-01

    Living cells are heterogeneous and rapidly changing biological samples. It is thus desirable to measure molecular concentration and dynamics in many locations at the same time. In this note, we present a multi-confocal setup capable of performing simultaneous fluorescence correlation spectroscopy measurements, by focusing the spots with a spatial light modulator and acquiring data with a monolithic 32 × 32 single-photon avalanche photodiode array. A post-processing method is proposed to correct cross-talk effects between neighboring spots. We demonstrate the applicability of our system by simultaneously measuring the diffusion of free enhanced Green Fluorescent Protein (eGFP) molecules at nine different points in living cells.

  5. MOTHER APP : a family memories theater

    OpenAIRE

    高, 磊; 奥出, 直人

    2011-01-01

    This thesis proposes MOTHER APP, an Application to Media Furniture Platform that creates a Family Memories Theater to document and exhibit shared household life experiences in the living room, automatically capturing spontaneous moments into photos by sensing if the family members are together through furniture. The thesis introduces the research, design process and implementation of MOTHER APP, describe the evalutation about how households use it to gain the new experience in the living room...

  6. Mothers' Mobility after Separation: Do Grandmothers Matter?

    Science.gov (United States)

    Das, Marjolijn; de Valk, Helga; Merz, Eva-Maria

    2017-03-01

    Starting from a life course perspective, this study aims to gain more insight into mobility patterns of recently separated mothers, focusing especially on moves to the location of their own mother: the maternal grandmother. Separated mothers, having linked lives with their own mothers, may benefit from their practical and emotional support. Additionally, the grandparents' home can be a (temporary) place to stay shortly after divorce. Data come from the System of social statistical datasets (Statistics Netherlands). This unique dataset combines longitudinal data from a vast number of administrative registers. It covers the complete Dutch population, making it exceptionally well suited for life course and mobility research. We studied mothers with minor children between 1/1/2008 and 31/12/2010. Our study included 579,500 mothers, of whom about 8,800 (1.5%) experienced a separation in 2008. Separated mothers moved to the grandmother's municipality more often than non-separated mothers, which might be partially motivated by the need for childcare. They also coresided with the grandmother more than non-separated movers, mostly because of a vulnerable socio-economic position. Although often temporary, coresidence appears to have a prolonged impact on the mothers' location choice; mothers frequently stayed in the grandmother's municipality after moving out. Finally, our results indicated that some mothers seemed to use the parental home as a stepping stone to cohabit with a new partner. © 2016 The Authors. Population, Space and Place published by John Wiley & Sons Ltd.

  7. Functional memory B cells and long-lived plasma cells are generated after a single Plasmodium chabaudi infection in mice.

    Directory of Open Access Journals (Sweden)

    Francis Maina Ndungu

    2009-12-01

    Full Text Available Antibodies have long been shown to play a critical role in naturally acquired immunity to malaria, but it has been suggested that Plasmodium-specific antibodies in humans may not be long lived. The cellular mechanisms underlying B cell and antibody responses are difficult to study in human infections; therefore, we have investigated the kinetics, duration and characteristics of the Plasmodium-specific memory B cell response in an infection of P. chabaudi in mice. Memory B cells and plasma cells specific for the C-terminal region of Merozoite Surface Protein 1 were detectable for more than eight months following primary infection. Furthermore, a classical memory response comprised predominantly of the T-cell dependent isotypes IgG2c, IgG2b and IgG1 was elicited upon rechallenge with the homologous parasite, confirming the generation of functional memory B cells. Using cyclophosphamide treatment to discriminate between long-lived and short-lived plasma cells, we demonstrated long-lived cells secreting Plasmodium-specific IgG in both bone marrow and in spleens of infected mice. The presence of these long-lived cells was independent of the presence of chronic infection, as removal of parasites with anti-malarial drugs had no impact on their numbers. Thus, in this model of malaria, both functional Plasmodium-specific memory B cells and long-lived plasma cells can be generated, suggesting that defects in generating these cell populations may not be the reason for generating short-lived antibody responses.

  8. A post-partum single-dose TDF/FTC tail does not prevent the selection of NNRTI resistance in women receiving pre-partum ZDV and intrapartum single-dose nevirapine to prevent mother-to- child HIV-1 transmission.

    Science.gov (United States)

    Samuel, Reshmi; Paredes, Roger; Parboosing, Raveen; Moodley, Pravi; Singh, Lavanya; Naidoo, Anneta; Gordon, Michelle

    2015-10-01

    Although the rates of vertical transmission of HIV in the developing world have improved to around 3% in countries like South Africa, resistance to antiretrovirals (ARV) used in Prevention of Mother-to-Child transmission (pMTCT) strategies may thwart such outcomes and affect the efficacy of future ARV regimens in mothers and children. This study conducted in Durban, South Africa, between 2010 and 2013 found a high rate of nevirapine (NVP) resistance among women receiving Zidovudine (AZT) from 14 weeks gestation, single dose nevirapine (sd NVP) at the onset of labor and a single dose of coformulated Tenofovir/Emtricitabine (TDF/FTC) postpartum. Using Sanger sequencing, high and intermediate levels of nevirapine (NVP) resistance were detected in 15/44 (34%) and in 1/44 (2%) of women tested, respectively. Most subjects selected the K103N mutation (22% (10/45) of all patients and 66% (10/15) of those with high-level NVP resistance). Such rate of NVP resistance is comparable to studies where only sd NVP was used. In conclusion, a post-partum single-dose TDF/FTC tail does not prevent the selection of NNRTI resistance in women receiving pre-partum ZDV and intrapartum sd NVP to prevent mother-to-child HIV-1 transmission. © 2015 Wiley Periodicals, Inc.

  9. Live-Cell Imaging Tool Optimization To Study Gene Expression Levels and Dynamics in Single Cells of Bacillus cereus

    NARCIS (Netherlands)

    Eijlander, Robyn T.; Kuipers, Oscar P.

    Single-cell methods are a powerful application in microbial research to study the molecular mechanism underlying phenotypic heterogeneity and cell-to-cell variability. Here, we describe the optimization and application of single-cell time-lapse fluorescence microscopy for the food spoilage bacterium

  10. Teenager-, Mother-, Daughter-, Who Am I? Navajo Adolescent Mothers' Perceptions of the Maternal Role & Implications for Child Developmental Outcomes.

    Science.gov (United States)

    Dalla, Rochelle L.

    This study explored the meaning of motherhood among Navajo teenagers, their mothers, and community informants living in a small, rural town on a Navajo Reservation. Participating were 8 Navajo teenage mothers ranging from 16 to 19 years, 7 grandmothers (mothers of the teens) who ranged from 41 to 57 years, and 6 community informants: two teachers,…

  11. Experiencing and the realization of motherhood by teenage mothers

    Directory of Open Access Journals (Sweden)

    Ewa Rzechowska

    2015-04-01

    Full Text Available Background Early motherhood constitutes a difficult challenge for girls, and the level of their performance in that role is varied. In this article, teenage motherhood as a process is considered. The objective of the research was to determine the paths by which teenage girls enter the mother role. Particular attention was paid to the nature of individual differences in the ways of experiencing and the realization of the successive steps of teenage motherhood: how the girls reacted to the fact of being a mother, what they experienced and how they behaved during pregnancy and performed child care. Participants and procedure In the research, 166 mothers who had given birth to their children between the 15th and 19th year of life were included (at the moment of giving birth to the child, the age of the mother was M = 17.22. A follower interview was used. It was directed towards recreating the course of their lives from the period preceding becoming pregnant to the period of pregnancy and looking after the child, taking into consideration the complex situations connected with life and development of the female teenagers. Results In the research, we applied the Reconstruction Strategy of the Process Transformation, setting the direction of qualitative analyses: (1 the level of single cases (case study, and (2 the level of the collection of cases (extracting groups of girls with common characteristics using the artificial intelligence algorithm C4.5. The analysis revealed the diversity and the internal structure of paths of the experience and realization of early motherhood: from negating oneself as a responsible mother to accepting the role of mother. Conclusions The final result is constituted by the model revealing the transformation of teenage motherhood and mechanisms underlying it.

  12. Communication and connectedness in mother- and father-adolescent relationships.

    Science.gov (United States)

    Youniss, J; Ketterlinus, R D

    1987-06-01

    In Study 1, 605 adolescents estimated how well their parents knew them and said how much they cared what their parents thought about them. Sons and daughters judged that mothers knew them fairly well, but daughters judged that fathers did not know them so well. At the same time, statements of caring indicated high concern by sons and daughters for both parents. A supplemental result was that sons from white-collar families gave relatively low estimates of how much their mothers knew them and daughters from blue-collar families gave very low estimates of how much their fathers knew them. In Study 2, 52 adolescents from single-parent families and living with their mothers but not with their fathers also gave estimates for knowing and caring. Knowing followed the above pattern, with an expected lowering of estimates for fathers by sons and daughters. Further, estimates of caring declined especially for fathers by daughters. These results add to the growing literature that shows mother-adolescent and father-adolescent relationships contribute differentially to psychological development. The results seem especially relevant for adolescents' sex role development and constructed individuality as mediated through relationships with both parents.

  13. Changes in glomerular filtration rate after donation in living kidney donors: a single-center cohort study.

    Science.gov (United States)

    Saito, Takako; Uchida, Keiko; Ishida, Hideki; Tanabe, Kazunari; Nitta, Kosaku

    2015-02-01

    A number of studies have reported on decline in glomerular filtration rate (GFR) after donation in Japanese living kidney donors. The purpose of the present study was to examine the clinicopathological factors associated with changes in GFR after donation in living kidney donors. We reviewed the charts of living kidney donors (n = 294) and monitored estimated GFR (eGFR) values from the time of 0-h kidney biopsy until 3 years after donation. We assessed donor age, gender, body mass index, blood pressure, urinalysis, and several other clinical parameters including the severity of glomerulosclerosis and arteriosclerosis. The grade of arteriosclerosis in 0-h biopsy specimens was higher in the older donor group (57-76 years) than in the younger donor group (30-56 years). Mean donor eGFR at the time of the donation was 80.1 ± 13.6 ml/min/1.73 m(2). Most of the living kidney donors in this study developed stage 3 chronic kidney disease (CKD). The mean changes in eGFR at 1-3 years after donation showed a steady state that was distinct from the generally accepted notion that GFR declines with age. Multivariate regression analyses showed that the changes in eGFR were negatively associated with age (r = -0.21, P < 0.001) and preoperative eGFR (r = -0.18, P < 0.001), but not associated with the grade of glomerulosclerosis and arteriosclerosis. Donor age and pre-GFR at the time of nephrectomy were associated with decline in kidney function in living kidney donors after donation. Most of the donors developed stage 3 CKD within 3 years after donation but without subsequent progression, at least for several years.

  14. Mother Trouble

    Directory of Open Access Journals (Sweden)

    Griselda Pollock

    2009-01-01

    Full Text Available In the late 1980s a practising painter who is also a practising psychoanalyst reflected upon the significance of events occurring in her painting and being reflected upon in her notebooks to evolve a major theoretical intervention in psychoanalytical thinking at the intersections of British Object Relations (Bion, Laing, Winnicott in which she was trained at the Tavistock and Parisian Lacanian and post Lacanian thinking (Laplanche, Guattari, Aulagnier, Dolto.  Supplementing the  then dominant understanding of Lacan's phallic Symbolic,  defined by the sovereignty of the phallus as the sole signifier, Bracha Ettinger proposed a further symbol, the Matrix and its non-phallic, non-Oedipal process, metramorphosis.  The matrixial enables us to catch up into theoretical knowledge another, shifting but not excluding dimension of  subjectivity that is the effect , on all subjects, irrespective of later, Oedipalised gender or sexuality, of the  feminine sexual specificity of human generation in the non-prohibited intimacy of the feminine-becoming-maternal-in co-emergence-with an-unknown-becoming-partial-other.  Moving beyond the theoretical engagements of object relations with early mother-child, hence post-natal relations between subjects, hence beyond intersubjectivity, Ettinger has been exploring, for almost two decades, the implications for theories of subjectivity and hence for ethics and even the politics of our multiple moments of transsubjective co-affections and co-effects, of the proposition that the feminine, understood as this sexual specificity of the severality of mutual co-effecting becoming of life, has something profound to offer our understanding of the human, its ethics, aesthetics and even politics.  Daring to theorize the gift to later subjectivities of the prolonged encounter-event between pre-natality and pre-maternity, Ettinger has contributed to debates about the maternal, the feminine and human subjectivity in general. In

  15. A Single-Center Retrospective Cohort Analysis of Maternal and Infant Outcomes in HIV-Infected Mothers Treated with Integrase Inhibitors During Pregnancy.

    Science.gov (United States)

    Mounce, Monique L; Pontiggia, Laura; Adams, Jessica L

    2017-12-01

    Integrase strand transfer inhibitors (INSTI) are currently being investigated for the treatment of HIV in pregnancy. The purpose of this study is to evaluate the differences in maternal and infant outcomes in HIV-positive mothers treated with INSTI-containing antiretroviral therapy (ART) during pregnancy compared to protease inhibitor (PI)-containing ART. A retrospective, cohort study of INSTI- and PI-based ART used in pregnancy between 2007 and 2015 was performed. The primary objective was to evaluate the differences in viral load (VL) suppression prior to delivery. Secondary endpoints included time to and duration of VL suppression and safety parameters in both mothers and infants. For the primary analysis, the two arms were matched 1:2 INSTI to PI based on the presence or absence of viremia at the time of pregnancy determination. Additional analysis was performed on the entire matched and unmatched dataset. Twenty-one patients were matched (7 INSTI and 14 PI). There were no significant differences between groups with respect to the proportion of patients with VL suppression prior to delivery (71.4% INSTI vs. 92.9% PI, p = 0.247), and there were no significant differences in any of the secondary endpoints. Patients with documented adherence issues were statistically more likely to not be virologically suppressed prior to delivery (p = 0.002). No differences in efficacy or safety were found between patients treated with INSTIs compared to PIs. This study supports the further investigation of the use of INSTIs during pregnancy to reduce HIV transmission.

  16. Role of ICAM-1 polymorphisms (G241R, K469E) in mediating its single-molecule binding ability: Atomic force microscopy measurements on living cells

    Energy Technology Data Exchange (ETDEWEB)

    Bai, Rui [Chinese (301) General Hospital, 28 Fuxing Road, Haidian District, Beijing 100853 (China); Yi, Shaoqiong [Beijing Institute of Biotechnology, 20 Dongdajie, Fengtai, Beijing 100071 (China); Zhang, Xuejie [Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry Chinese Academy of Sciences, 2 Zhongguancun North 1st Street, Beijing 100190 (China); Liu, Huiliang, E-mail: lhl518@vip.sina.com [Department of Cardiology, The General Hospital of Chinese People’s Armed Police Forces, Beijing 100039 (China); Fang, Xiaohong, E-mail: xfang@iccas.ac.cn [Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry Chinese Academy of Sciences, 2 Zhongguancun North 1st Street, Beijing 100190 (China)

    2014-06-13

    Highlights: • We evaluated both single molecule binding ability and expression level of 4 ICAM-1 mutations. • AFM was used to measure single-molecule binding ability on living cells. • The SNP of ICAM-1 may induce changes in expressions rather than single-molecule binding ability. - Abstract: Atherosclerosis (As) is characterized by chronic inflammation and is a major cause of human mortality. ICAM-1-mediated adhesion of leukocytes in vessel walls plays an important role in the pathogenesis of atherosclerosis. Two single nucleotide polymorphisms (SNPs) of human intercellular adhesion molecule-1 (ICAM-1), G241R and K469E, are associated with a number of inflammatory diseases. SNP induced changes in ICAM-1 function rely not only on the expression level but also on the single-molecule binding ability which may be affected by single molecule conformation variations such as protein splicing and folding. Previous studies have shown associations between G241R/K469E polymorphisms and ICAM-1 gene expression. Nevertheless, few studies have been done that focus on the single-molecule forces of the above SNPs and their ligands. In the current study, we evaluated both single molecule binding ability and expression level of 4 ICAM-1 mutations – GK (G241/K469), GE (G241/E469), RK (R241/K469) and RE (R241/E469). No difference in adhesion ability was observed via cell adhesion assay or atomic force microscopy (AFM) measurement when comparing the GK, GE, RK, or RE genotypes of ICAM-1 to each other. On the other hand, flow cytometry suggested that there was significantly higher expression of GE genotype of ICAM-1 on transfected CHO cells. Thus, we concluded that genetic susceptibility to diseases related to ICAM-1 polymorphisms, G241R or K469E, might be due to the different expressions of ICAM-1 variants rather than to the single-molecule binding ability of ICAM-1.

  17. “We Live From Mother Nature”

    Directory of Open Access Journals (Sweden)

    J. Marcela Chaves-Agudelo

    2015-08-01

    Full Text Available This article explores how macroeconomic and environmental policies instituted since the 1990s have altered meanings, imaginaries, and the human relationship to nature in Colombia. The Colombian nation-state is pluri-ethnic, multilingual, and megabiodiverse. In this context, indigenous peoples, Afro-Colombians, and some peasant communities survive hybridization of their cultures. They have developed their own ways of seeing, understanding, and empowering the world over centuries of European rule. However, threats to relatively discrete cultural meanings have increased since major changes in the 1990s, when Colombia experienced the emergence of new and modern interpretations of nature, such as “biodiversity,” and a deepening of globalized neoliberal economic and political management. These policies involve a modern logic of being in the world, the establishment of particular regulatory functions for economies, societies, and the environment, and their spread has been facilitated by webs of political and economic power. We trace their local effects with reference to three indigenous groups.

  18. Long-term health-related quality of life of living kidney donors: a single-center experience.

    Science.gov (United States)

    Benzing, Christian; Hau, Hans-Michael; Kurtz, Greta; Schmelzle, Moritz; Tautenhahn, Hans-Michael; Morgül, Mehmet Haluk; Wiltberger, Georg; Broschewitz, Johannes; Atanasov, Georgi; Bachmann, Anette; Bartels, Michael

    2015-12-01

    Over the last few years, the evaluation of the health-related quality of life (HRQoL) of living kidney donors (LKD) has become of particular interest. The present study sought to evaluate the physical and mental HRQoL after kidney removal. The clinical and paraclinical course of these patients was examined, and the impact of preoperative donor evaluation, donor nephrectomy, and surgical recovery was evaluated. These data were compared with reference data of the general population. Between 1998 and 2010, 72 living kidney donations were performed at our institution. To assess the HRQoL, two questionnaires-the Short Form 36 (SF-36) and a special LKD questionnaire-were sent to all 72 living donors. The records of the follow-up examinations of all 72 donors were retrospectively analyzed in order to assess the clinical and paraclinical data after kidney donation. Out of 72 donors, 55 (76.4 %) responded to the questionnaires. There was no change in systolic and diastolic blood pressure during the 7-year follow-up (p > 0.05). Mild proteinuria (>150 mg/l) was observed in six cases. Kidney donors had a higher HRQoL compared to the general population with mean values of the physical and mental summation scale (PCS and MCS, respectively) being 51.3 (SD = 7.6) and 50.6 (SD = 8.1). Peri- or postoperative complications were associated with lower values for physical function and physical component summary (PCS) (p < 0.05). Living donor kidney transplantation appears to be safe for donors. The HRQoL is excellent. To ensure a positive outcome for donors, a good clinical evaluation of potential donors is essential.

  19. Migrant women living with HIV in Europe: are they facing inequalities in the prevention of mother-to-child-transmission of HIV?: The European Pregnancy and Paediatric HIV Cohort Collaboration (EPPICC) study group in EuroCoord.

    Science.gov (United States)

    Favarato, G; Bailey, H; Burns, F; Prieto, L; Soriano-Arandes, A; Thorne, C

    2018-02-01

    In pregnancy early interventions are recommended for prevention of mother-to-child-transmission (PMTCT) of HIV. We examined whether pregnant women who live with HIV in Europe and are migrants encounter barriers in accessing HIV testing and care. Four cohorts within the European Pregnancy and Paediatric HIV Cohort Collaboration provided data for pooled analysis of 11 795 pregnant women who delivered in 2002-12 across ten European countries. We defined a migrant as a woman delivering in a country different from her country of birth and grouped the countries into seven world regions. We compared three suboptimal PMTCT interventions (HIV diagnosis in late pregnancy in women undiagnosed at conception, late anti-retroviral therapy (ART) start in women diagnosed but untreated at conception and detectable viral load (VL) at delivery in women on antenatal ART) in native and migrant women using multivariable logistic regression models. Data included 9421 (79.9%) migrant women, mainly from sub-Saharan Africa (SSA); 4134 migrant women were diagnosed in the current pregnancy, often (48.6%) presenting with CD4 count <350 cells/µl. Being a migrant was associated with HIV diagnosis in late pregnancy [OR for SSA vs. native women, 2.12 (95% CI 1.67, 2.69)] but not with late ART start if diagnosed but not on ART at conception, or with detectable VL at delivery once on ART. Migrant women were more likely to be diagnosed in late pregnancy but once on ART virological response was good. Good access to antenatal care enables the implementation of PMTCT protocols and optimises both maternal and children health outcomes generally. © The Author 2017. Published by Oxford University Press on behalf of the European Public Health Association. All rights reserved.

  20. Vivenciando com o filho uma passagem difícil e reveladora: a experiência da mãe acompanhante Experiencing a difficult and revealing passage with her child: the live-in mother experience

    Directory of Open Access Journals (Sweden)

    Irma de Oliveira

    2000-06-01

    Full Text Available O presente estudo foi realizado com mães acompanhantes em um hospital escola. Os objetivos foram: compreender as interações vivenciadas, identificar os significados que o familiar acompanhante atribui à experiência de vivenciar a hospitalização da criança, identificar o que essa vivência provoca na vida do familiar e construir um modelo teórico representativo da experiência. Utilizou-se como Referencial Teórico o Interacionismo Simbólico e Referencial Metodológico a "Grounded Theory". Identificou-se os fenômenos Indo em busca de solução e Atravessando uma situação difícil. A partir desses fenômenos emergiu a categoria central Vivenciando com o filho uma passagem difícil e reveladora.This study was conducted with live-in mother in university hospital. The objectives were as follows: to understand the experienced interactions, to identify the meanings that a family attribute to the experience of going through the child's hospitalization, to identify what the experience of hospitalization provokes in the family life and construct a model theory representative of the experience. Reference Theory the Symbolic Interactionism and Reference Methodology Grounded Theory had been utilized. From results appeared the phenomenon Going in search of solution, and Going through a difficult situation. Through these phenomena, the main category Experiencing a difficult and revealing passage with her child was identified.

  1. iSBatch: a batch-processing platform for data analysis and exploration of live-cell single-molecule microscopy images and other hierarchical datasets.

    Science.gov (United States)

    Caldas, Victor E A; Punter, Christiaan M; Ghodke, Harshad; Robinson, Andrew; van Oijen, Antoine M

    2015-10-01

    Recent technical advances have made it possible to visualize single molecules inside live cells. Microscopes with single-molecule sensitivity enable the imaging of low-abundance proteins, allowing for a quantitative characterization of molecular properties. Such data sets contain information on a wide spectrum of important molecular properties, with different aspects highlighted in different imaging strategies. The time-lapsed acquisition of images provides information on protein dynamics over long time scales, giving insight into expression dynamics and localization properties. Rapid burst imaging reveals properties of individual molecules in real-time, informing on their diffusion characteristics, binding dynamics and stoichiometries within complexes. This richness of information, however, adds significant complexity to analysis protocols. In general, large datasets of images must be collected and processed in order to produce statistically robust results and identify rare events. More importantly, as live-cell single-molecule measurements remain on the cutting edge of imaging, few protocols for analysis have been established and thus analysis strategies often need to be explored for each individual scenario. Existing analysis packages are geared towards either single-cell imaging data or in vitro single-molecule data and typically operate with highly specific algorithms developed for particular situations. Our tool, iSBatch, instead allows users to exploit the inherent flexibility of the popular open-source package ImageJ, providing a hierarchical framework in which existing plugins or custom macros may be executed over entire datasets or portions thereof. This strategy affords users freedom to explore new analysis protocols within large imaging datasets, while maintaining hierarchical relationships between experiments, samples, fields of view, cells, and individual molecules.

  2. Estimating protein-protein interaction affinity in single living cells using Förster resonance energy transfer measurements

    DEFF Research Database (Denmark)

    Jensen, Jens Ledet; Raarup, Merete Krog; Rubak, Ege

    Using Förster resonance energy transfer (FRET) images we study the possibility of estimating the equilibrium dissociation constant Kd and the intrinsic FRET efficiency Em from single cells. We model the measurement uncertainty in the acquired images and use the method of total least squares...

  3. The Chinese Tiger Mother

    Directory of Open Access Journals (Sweden)

    Jacek Hołówka

    2011-12-01

    Full Text Available In 2010 a book by Amy Chua: Battle Hymn of the Tiger Mother was published and it sparked a broad discussion among pedagogues and the open society about the factors determining educational success. Chua forms a simple and provocative thesis – the Chinese mothers are the best in the world because they don’t spoil their children, quickly introduce them into the adult culture, have high expectations of them, they are brusque and cold but they teach their children how to survive and be competitive. Chua shows this educational model as a contrast to the Western model, where the children have their own, naive and sentimental culture, their own shops and catwalks in shopping malls. The results of systematic research on education seem to prove something quite different. The educational success has to be measured using different scales, because it depends on different factors. The data published by OECD show that the level of education depends on the educational tradition of the society, level of GDP, intergenerational contacts, level of education of teachers and their social status. A strong determiner is the family, but not necessarily the mother. Even more, there is a strong correlation between the results in learning and a supporting stance of the parents, but also with their habit of spending free time with their children. The parents who take their children to the cinema, an a trip, gossip with them or take them to McDonalds, can be sure that their children will have statistically higher than the average grades. Detailed results from other sources show that success correlates the most with grades from previous class, parents’ habit of talking about things at school, higher education of the parents, being a child of a single mother, signing the child up for extracurricular classes from music and the mother working part-time. Failure correlates with being an Afro-American or Latino, checking homework by parents, free time after school without

  4. Lone mothers in Sweden

    DEFF Research Database (Denmark)

    Burström, B; Diderichsen, Finn; Shouls, S

    1999-01-01

    To study trends in the health and socioeconomic circumstances of lone mothers in Sweden over the years 1979-1995, and to make comparisons with couple mothers over the same period.......To study trends in the health and socioeconomic circumstances of lone mothers in Sweden over the years 1979-1995, and to make comparisons with couple mothers over the same period....

  5. Untraditional caring arrangements among parents living apart: The case of Norway

    Directory of Open Access Journals (Sweden)

    Ragni Hege Kitterød

    2012-07-01

    Full Text Available BACKGROUND In spite of quite symmetric parental roles in Norway, shared residence and father sole custody are still rare when parents split up. Several countries have witnessed an increase in shared residence for children recently, and this is also the case in Norway. OBJECTIVE We wish to add to the literature on untraditional caring arrangements among parents living apart by examining the determinants of shared residence and sole father custody in Norway, a country with high gender-equality ambitions. METHODS Based on a survey from 2004 with a unique sample of former couples, we ran multinomial logistic regressions estimating the odds of shared residence rather than mother sole custody, and the odds of father sole custody rather than mother sole custody. RESULTS Shared residence is particularly likely when the father has a reasonable income, the mother is highly educated, the mother is currently married, and the parents have no other children in their households. Father sole custody is most likely when the mother's income is low and the father's high, the child is a boy and at least ten years old, the father is single and there are other children in the mother's household. CONCLUSIONS Despite more equal parental roles in couples in recent decades, most children still live mainly with their mother when parents split up in Norway. However, visiting arrangements with fathers are extensive. More parents will probably opt for shared residence in the years to come.

  6. High-Speed Single Quantum Dot Imaging of Artificial Lipids in Live Cells Reveal Partial Hop Diffusion

    DEFF Research Database (Denmark)

    Lagerholm, B. Christoffer; Clausen, Mathias P.; Christensen, Eva Arnspang

    2010-01-01

    Ultra high-speed single particle tracking (image frame rates 40-50,000 Hz) experiments with 40 nm gold particles has indicated that lipids and proteins in the plasma membrane undergo hop-diffusion between nanometer sized compartments (Fujiwara et al. (2002) J Cell Biol. 157: 1071......-81). These findings have yet to be independently confirmed. In this work, we show that high-speed single particle tracking with quantum dots(QDs)and using a standard wide-field fluorescence microscope and an EMCCD is possible at image acquisition rates of up to ~2000 Hz with an image integration time of ~0.5 msec....... The spatial precision in these experiments is ~40 nm (as determined from the standard deviation of repeated position measurements of an immobile QD on a cell). Using this system, we further show that an artificial lipid, biotin-cap-DPPE, inserted in a mouse embryo fibroblast (MEF), labeled with sAv-QD655...

  7. Electrochemical monitoring of intracellular enzyme activity of single living mammalian cells by using a double-mediator system

    International Nuclear Information System (INIS)

    Matsumae, Yoshiharu; Takahashi, Yasufumi; Ino, Kosuke; Shiku, Hitoshi; Matsue, Tomokazu

    2014-01-01

    Graphical abstract: NAD(P)H:quinone oxidoreductase (NQO) activity of single HeLa cells were evaluated by using the menadione–ferrocyanide double mediator system combined with scanning electrochemical microscopy (SECM). - Highlights: • NAD(P)H:quinone oxidoreductase activity of single cells were evaluated with SECM. • Fe(CN) 6 3− /menadione concentrations were optimized for long-term SECM monitoring. • Menadione affect the intracellular levels of reactive oxygen species and GSH. • At 100 μM menadione, the Fe(CN) 6 3− generation rate decreased rapidly within 30 min. - Abstract: We evaluated the intracellular NAD(P)H:quinone oxidoreductase (NQO) activity of single HeLa cells by using the menadione–ferrocyanide double-mediator system combined with scanning electrochemical microscopy (SECM). The double-mediator system was used to amplify the current response from the intracellular NQO activity and to reduce menadione-induced cell damage. The electron shuttle between the electrode and menadione was mediated by the ferrocyanide/ferricyanide redox couple. Generation of ferrocyanide was observed immediately after the addition of a lower concentration (10 μM) of menadione. The ferrocyanide generation rate was constant for 120 min. At a higher menadione concentration (100 μM), the ferrocyanide generation rate decreased within 30 min because of the cytotoxic effect of menadione. We also investigated the relationship between intracellular reactive oxygen species or glutathione levels and exposure to different menadione concentrations to determine the optimal condition for SECM with minimal invasiveness. The present study clearly demonstrates that SECM is useful for the analysis of intracellular enzymatic activities in single cells with a double-mediator system

  8. Electrochemical monitoring of intracellular enzyme activity of single living mammalian cells by using a double-mediator system

    Energy Technology Data Exchange (ETDEWEB)

    Matsumae, Yoshiharu [Graduate School of Environmental Studies, Tohoku University, Aramaki 6-6-11-605, Aoba, Sendai 980-8579 (Japan); Takahashi, Yasufumi [Advanced Institute for Materials Research, Tohoku University, Katahira 2-1-1, Aoba, Sendai 980-8577 (Japan); Ino, Kosuke [Graduate School of Environmental Studies, Tohoku University, Aramaki 6-6-11-605, Aoba, Sendai 980-8579 (Japan); Shiku, Hitoshi, E-mail: shiku@bioinfo.che.tohoku.ac.jp [Graduate School of Environmental Studies, Tohoku University, Aramaki 6-6-11-605, Aoba, Sendai 980-8579 (Japan); Matsue, Tomokazu, E-mail: matsue@bioinfo.che.tohoku.ac.jp [Graduate School of Environmental Studies, Tohoku University, Aramaki 6-6-11-605, Aoba, Sendai 980-8579 (Japan); Advanced Institute for Materials Research, Tohoku University, Katahira 2-1-1, Aoba, Sendai 980-8577 (Japan)

    2014-09-09

    Graphical abstract: NAD(P)H:quinone oxidoreductase (NQO) activity of single HeLa cells were evaluated by using the menadione–ferrocyanide double mediator system combined with scanning electrochemical microscopy (SECM). - Highlights: • NAD(P)H:quinone oxidoreductase activity of single cells were evaluated with SECM. • Fe(CN){sub 6}{sup 3−}/menadione concentrations were optimized for long-term SECM monitoring. • Menadione affect the intracellular levels of reactive oxygen species and GSH. • At 100 μM menadione, the Fe(CN){sub 6}{sup 3−} generation rate decreased rapidly within 30 min. - Abstract: We evaluated the intracellular NAD(P)H:quinone oxidoreductase (NQO) activity of single HeLa cells by using the menadione–ferrocyanide double-mediator system combined with scanning electrochemical microscopy (SECM). The double-mediator system was used to amplify the current response from the intracellular NQO activity and to reduce menadione-induced cell damage. The electron shuttle between the electrode and menadione was mediated by the ferrocyanide/ferricyanide redox couple. Generation of ferrocyanide was observed immediately after the addition of a lower concentration (10 μM) of menadione. The ferrocyanide generation rate was constant for 120 min. At a higher menadione concentration (100 μM), the ferrocyanide generation rate decreased within 30 min because of the cytotoxic effect of menadione. We also investigated the relationship between intracellular reactive oxygen species or glutathione levels and exposure to different menadione concentrations to determine the optimal condition for SECM with minimal invasiveness. The present study clearly demonstrates that SECM is useful for the analysis of intracellular enzymatic activities in single cells with a double-mediator system.

  9. Markers of Decompression Stress of Mass Stranded/Live Caught and Released vs. Single Stranded Marine Mammals

    Science.gov (United States)

    2014-09-30

    Caught and Released vs. Single Stranded Marine Mammals Michael Moore Biology Department Woods Hole Oceanographic Institution Woods Hole, MA 02543...Society for Marine Mammalogy 2013 Biennial Conference on the Biology of Marine Mammals in New Zealand. Dr. Fahlman’s graduate student Lauren Gonzalez...Harabin, Metabolism and thermoregulation in guinea pigs in hyperbaric hydrogen: Effects of pressure. Journal of Thermal Biology , 1997. 22(1): p. 31-41

  10. A microfluidic-enabled mechanical microcompressor for the immobilization of live single- and multi-cellular specimens.

    Science.gov (United States)

    Yan, Yingjun; Jiang, Liwei; Aufderheide, Karl J; Wright, Gus A; Terekhov, Alexander; Costa, Lino; Qin, Kevin; McCleery, W Tyler; Fellenstein, John J; Ustione, Alessandro; Robertson, J Brian; Johnson, Carl Hirschie; Piston, David W; Hutson, M Shane; Wikswo, John P; Hofmeister, William; Janetopoulos, Chris

    2014-02-01

    A microcompressor is a precision mechanical device that flattens and immobilizes living cells and small organisms for optical microscopy, allowing enhanced visualization of sub-cellular structures and organelles. We have developed an easily fabricated device, which can be equipped with microfluidics, permitting the addition of media or chemicals during observation. This device can be used on both upright and inverted microscopes. The apparatus permits micrometer precision flattening for nondestructive immobilization of specimens as small as a bacterium, while also accommodating larger specimens, such as Caenorhabditis elegans, for long-term observations. The compressor mount is removable and allows easy specimen addition and recovery for later observation. Several customized specimen beds can be incorporated into the base. To demonstrate the capabilities of the device, we have imaged numerous cellular events in several protozoan species, in yeast cells, and in Drosophila melanogaster embryos. We have been able to document previously unreported events, and also perform photobleaching experiments, in conjugating Tetrahymena thermophila.

  11. Mothering and acculturation: experiences during pregnancy and childrearing of Filipina mothers married to Japanese.

    Science.gov (United States)

    Uayan, Maria Luisa Tumandao; Kobayashi, Sayuri; Matsuzaki, Masayo; Ota, Erika; Haruna, Megumi; Murashima, Sachiyo

    2009-06-01

    This study aims to describe the lived experiences of Filipina mothers married to Japanese during pregnancy and childrearing. Eight focus group interviews (FGI) were conducted among 39 Filipino mothers who are currently residing in Japan to obtain significant information with regard to their pregnancy and childrearing experiences. Content analysis was used to extract relevant themes that will describe the experiences of this group of migrant mothers. The findings revealed three major themes: 1) cultural barriers during pregnancy and childrearing; 2) mothering at the different stages of childrearing; and 3) positive adjustments to a new role in a new environment. The establishment of means of communication with migrant mothers effectively enabling them to understand important information for promoting healthy pregnancy and childrearing is strongly recommended. Provision of school information in the English language and enhancing the resilient character of the Filipina are important strategies in promoting positive pregnancy and childrearing experiences among Filipina migrant mothers.

  12. Living uterus donation and transplantation: experience of interest and screening in a single center in the United States.

    Science.gov (United States)

    Johannesson, Liza; Wallis, Kristin; Koon, E Colin; McKenna, Greg J; Anthony, Tiffany; Leffingwell, Sara G; Klintmalm, Goran B; Gunby, Robert T; Testa, Giuliano

    2018-03-01

    Little is known about attitudes toward uterus donation and transplantation in society and the interest of the women the treatment is aimed to assist. This study examined the interest of recipients and living donors in our uterus transplantation program; it describes the screening protocol we developed and the results of the screening and reports demographic data and characteristics of screened candidates. Initial screening and evaluation included physical examinations by a gynecologist and a transplant surgeon; psychological evaluation; imaging (x-ray, computed tomography, ultrasound); blood tests; immunological testing; viral, bacterial, and fungal testing; drug screen; hormonal testing; Papanicolau smear; urinalysis; and electrocardiogram. For selected recipients, the process also included in vitro fertilization. A total of 351 women contacted our department with interest in participating in uterus transplantation; 272 were potential recipients and 79 were potential donors. Among these women, 179 potential recipients and 62 potential donors continued the evaluation after the initial telephone screening. The mean age of the donor candidates was 40 years; all had completed their own family, and 80% were nondirected. Most recipient candidates (92%) had an anatomical lack of the uterus, and of these, 36% had a congenital malformation. The women with a congenital uterine absence were in general younger than the women in the group whose uterus had been removed (mean of 28 and 33 years, respectively). In every step of the initial screening and evaluation process, there were donor and recipient candidates that chose not to continue the process. The reasons for self-withdrawal after expressing interest were not returning phone calls or e-mails (17 donors and 76 recipients); after initial phone screening, no longer interested (1 donor and 9 recipients); in step 1, health history questionnaire not returned after 1 reminder (10 donors and 9 recipients); step 2, not right in

  13. Adult outcomes of teen mothers across birth cohorts

    OpenAIRE

    Anne Driscoll

    2014-01-01

    Background: Teen and young adult mothers have lower socioeconomic status than older mothers. Objective: This study analyzes the socioeconomic status (SES) of teen, young adult, and older adult mothers across four five-year birth cohorts from 1956 to 1975 who were teens from 1971 to 1994. Methods: Data were pooled from the 1995, 2002, and 2006-2010 National Survey of Family Growth (NSFG). Mothers were categorized by age at first birth and by their birth cohorts. The SES (education, singl...

  14. Multiple birth families with children with special needs: a qualitative investigation of mothers' experiences.

    Science.gov (United States)

    Bolch, Christie E; Davis, Peter G; Umstad, Mark P; Fisher, Jane R W

    2012-08-01

    Multiple birth remains prevalent, with prematurity and subsequent disability being common complications. However, little is known of the experiences of mothers living with the combined circumstances of multiple birth, prematurity, and special needs. This paper reports an exploratory study using the qualitative technique of thematic analysis, to describe and interpret the experiences of 10 mothers of prematurely born multiple birth children with diverse special needs. Mothers were shocked to learn they were carrying multiple fetuses, including those who underwent in vitro fertilization with dual embryo transfer. Most experienced protracted concern over one or more babies' survival during pregnancy, and prescribed bed rest was frequently associated with increased anxiety and other adverse psychological effects. Some experienced disenfranchised grief, such as those grieving the opportunity to bear a single child. The contrast with experiences of mothers of healthy, term singletons caused considerable distress. Feelings of detachment and unreality were common in the immediate postpartum period, possibly due to transient depersonalization. Having more than one newborn created practical and psychological problems during the neonatal period, particularly when infants were separated due to differences in medical status. The extent to which hospitals accommodated the multiple birth relationship varied and significantly affected mothers' postnatal experiences. Mothers often felt guilty, particularly regarding inequality of care and attention they were able to provide to each child. This was especially problematic for multiples discordant for special needs status. The presence of one normally developing child complicated adjustment to the other's difficulties, and several experienced chronic sorrow. Serious maternal mental health difficulties were common but not universal. Available formal supports were generally perceived as inadequate, addressing some, but not all, of the

  15. Widowed mothers' coresidence with adult children.

    Science.gov (United States)

    Seltzer, Judith A; Friedman, Esther M

    2014-01-01

    Coresidence is one way that middle-aged offspring assist vulnerable, aging parents. This study investigated which characteristics of widowed mothers and adult children predict coresidence. When coresidence occurred, the analysis explored how individual children's characteristics were associated with their coresidence with the mother. Survey data from adults 53-54 years old in 1993 (N = 2,324) and a random sibling reported about their living situation, other siblings, and their mother, median age 80. Logistic regressions revealed that mothers in poor health, who were older, and who had a daughter were more likely to live with a child. Among coresiding families, results from discrete choice conditional logit models showed that widowed mothers were more likely to live with an unmarried son than an unmarried daughter. Married children were less likely to coreside than unmarried children, but married daughters were more likely than married sons to coreside. Past receipt of financial help from parents was not associated with coresidence. Coresidence was more likely for those with a close relationship with the mother. The discussion considers coresidence as an intergenerational transfer and its importance for the contemporary aging society. Data are needed on characteristics of all offspring to test theories about parent-child relationships.

  16. A highly sensitive, single selective, fluorescent sensor for Al3+ detection and its application in living cell imaging

    International Nuclear Information System (INIS)

    Ye, Xing-Pei; Sun, Shao-bo; Li, Ying-dong; Zhi, Li-hua; Wu, Wei-na; Wang, Yuan

    2014-01-01

    A new o-aminophenol-based fluorogenic chemosensor methyl 3,5-bis((E)-(2-hydroxyphenylimino)methyl)-4-hydroxybenzoate 1 have been synthesized by Schiff base condensation of methyl 3,5-diformyl-4-hydroxybenzoate with o-aminophenol, which exhibits high selectivity and sensitivity toward Al 3+ . Fluorescence titration studies of receptors 1 with different metal cations in CH 3 OH medium showed highly selective and sensitive towards Al 3+ ions even in the presence of other commonly coexisting metal ions. The detection limit of Al 3+ ions is at the parts per billion level. Interestingly, the Al(III) complex of 1 offered a large Stokes shift (>120 nm), which can miximize the selfquenching effect. In addition, possible utilization of this receptor as bio-imaging fluorescent probe to detect Al 3+ in human cervical HeLa cancer cell lines was also investigated by confocal fluorescence microscopy. - Highlights: • A new Schiff base chemosensor is reported. • The sensor for Al 3+ offers large Stokes shift. • The detection limit of Al 3+ in CH 3 OH solution is at the parts per billion level. • The utilization of sensor for the monitoring of Al 3+ levels in living cells was examined

  17. A highly sensitive, single selective, fluorescent sensor for Al{sup 3+} detection and its application in living cell imaging

    Energy Technology Data Exchange (ETDEWEB)

    Ye, Xing-Pei [Department of Physics and Chemistry, Henan Polytechnic University, Jiaozuo 454000 (China); Sun, Shao-bo; Li, Ying-dong [Institute of Integrated Traditional and Western Medicine, Gansu University of Traditional Chinese Medicine, Lanzhou 730000 (China); Zhi, Li-hua [Department of Physics and Chemistry, Henan Polytechnic University, Jiaozuo 454000 (China); Wu, Wei-na, E-mail: wuwn08@hpu.edu.cn [Department of Physics and Chemistry, Henan Polytechnic University, Jiaozuo 454000 (China); Wang, Yuan, E-mail: wangyuan08@hpu.edu.cn [Department of Physics and Chemistry, Henan Polytechnic University, Jiaozuo 454000 (China)

    2014-11-15

    A new o-aminophenol-based fluorogenic chemosensor methyl 3,5-bis((E)-(2-hydroxyphenylimino)methyl)-4-hydroxybenzoate 1 have been synthesized by Schiff base condensation of methyl 3,5-diformyl-4-hydroxybenzoate with o-aminophenol, which exhibits high selectivity and sensitivity toward Al{sup 3+}. Fluorescence titration studies of receptors 1 with different metal cations in CH{sub 3}OH medium showed highly selective and sensitive towards Al{sup 3+} ions even in the presence of other commonly coexisting metal ions. The detection limit of Al{sup 3+} ions is at the parts per billion level. Interestingly, the Al(III) complex of 1 offered a large Stokes shift (>120 nm), which can miximize the selfquenching effect. In addition, possible utilization of this receptor as bio-imaging fluorescent probe to detect Al{sup 3+} in human cervical HeLa cancer cell lines was also investigated by confocal fluorescence microscopy. - Highlights: • A new Schiff base chemosensor is reported. • The sensor for Al{sup 3+} offers large Stokes shift. • The detection limit of Al{sup 3+} in CH{sub 3}OH solution is at the parts per billion level. • The utilization of sensor for the monitoring of Al{sup 3+} levels in living cells was examined.

  18. Lone mothers and their network support: Sociodemographic research of nonmarital parenthood in Serbia

    Directory of Open Access Journals (Sweden)

    Stanković Biljana

    2014-01-01

    Full Text Available The aim of this paper is to identify and describe the difficulties faced by families with nonmarital children (especially single-parent, available help and support, participation of the father in raising a child, and expected institutional help. That represents a first step toward better understanding of these families. The paper presents part of the results of research carried out in Belgrade with the aim of insight into the phenomenon of nonmarital childbearing at the individual level. It was conducted on a sample of 100 respondents, mothers of nonmarital children, who attend preschool. The survey was anonymous, carried out by the interview method. For this purpose a questionnaire with more than 50 questions was made, standardized to a great extent. In this paper, the focus is on the hardships and needs of the group of lone mothers. Though some issues take into account the answers of all respondents (21 cohabited mothers and 79 lone mothers, the analysis is largely related to the experiences and reflections of lone mothers. The 2011 Census data on prevalence of consensual unions and characteristics of persons living in them, as well as earlier data on the acknowledgment of paternity, do not indicate that nonmarital childbearing in Serbia takes place primarily in stable unions. It is realistic to assume that a large percentage of mothers with nonmarital children are lone mothers. The research results are generally consistent with the findings of relevant studies from our and foreign countries. As the largest difficulties, lone mothers emphasize financial problems, unemployment, housing difficulties, and child care, as well as a pronounced feeling of loneliness. Most of them in solving problems and rising children rely on their parents and siblings, with whom they often live in the same household. Every fifth lone mother when faced with a major problem, and every ninth in everyday raising a child can count on the father of the child. About a

  19. Dynamics of intracellular ionic concentrations in single living cells using videomicrofluorometry: application to pHi variations

    Science.gov (United States)

    Viallet, Pierre M.; Yassine, Mohamed; Salmon, Jean-Marie; Vigo, Jean

    1996-01-01

    The intracellular concentration of ions such as H+, Mg2+, Ca2+, is known to monitor the activity of many fundamental enzymes. Furthermore these ions are generally considered as intracellular messengers involved in the transduction of extracellular signals. Recent technological progress, occulting the physicochemical properties of the probe, led to the feeling that accurate data on microvolumes are instantly accessible. Unfortunately fluorescent probes are supposed to fill up conflicting requirements for ionic affinity, absence of fading and intracellular calibration. Such a situation generally precludes the use of the simplest methods of data acquisition and treatment. This paper is based on the use of microspectrofluorometry, resolution of single cell complex fluorescence spectrum, and videomicrofluorometry. The methods of data handling allow us to demonstrate that most of the problems met in intracellular calibration come from the fighting of cells against the modification of the extracellular pH. Using these techniques allows us to restrict the need of comparison between results in cuvettes and intracellular results to the physiological pH range. A consequence of such an approach is that the effect with time of known concentrations of amiloride and nigericin on pHi became accessible. Data is presented allowing us to get information on the behavior of the ionic channels and/or cation/H+ exchangers involved in the pHi regulation. Such a method leads the way to direct investigations and monitoring of the different processes of regulation of the intracellular ionic concentrations in different cell lines at the level of single cells. Using different specific modifiers (activators or blocking agents) and convenient specific fluorescent probes, the efficiency of such pathways is expected to be checked at will. Compared to the patch clamp techniques, the method can be extended to the study of pathways located on the inner cell membranes.

  20. Bile duct evaluation of potential living liver donors with Gd-EOB-DTPA enhanced MR cholangiography: Single-dose, double dose or half-dose contrast enhanced imaging

    International Nuclear Information System (INIS)

    Kinner, Sonja; Steinweg, Verena; Maderwald, Stefan; Radtke, Arnold; Sotiropoulos, Georgios; Forsting, Michael; Schroeder, Tobias

    2014-01-01

    Introduction: Detailed knowledge of the biliary anatomy is essential to avoid complications in living donor liver transplantation. The aim of this study was to determine the optimal dosage of Gd-EOB-DTPA for contrast-enhanced magnetic resonance cholangiography (ce-MRC) with reference to contrast-enhanced CT cholangiography (ce-CTC). Materials and methods: 30 potential living liver donors (PLLD) underwent both ce-CTC and ce-MRC. Ten candidates each received single, double or half-dose Gd-EOB-DTPA. Ce-MRC images with and without inversion recovery pulses (T1w ± IR) were acquired 20–30 min after intravenous contrast injection. Image data was quantitatively and qualitatively reviewed by two radiologists based on a on a 5-point scale. Data sets were compared using a Mann–Whitney-U-test or Wilcoxon-rank-sum-test. Kappa values were also calculated. Results: All image series provided sufficient diagnostic information both showing normal biliary anatomy and variant bile ducts. Ce-CTC showed statistically significant better results compared to all ce-MRC data sets. T1w MRC with single dose Gd-EOB-DTPA proved to be superior to half and double dose in subjective and objective evaluation without a statistically significant difference. Conclusions: Ce-MRC is at any dosage inferior to ce-CTC. As far as preoperative planning of bile duct surgery is focused on the central biliary anatomy, ce-MRC can replace harmful ce-CTC strategies, anyway. Best results were seen with single dose GD-EOB-DTPA on T1w MRC+IR

  1. Impact of singlehood during pregnancy on dietary intake and birth outcomes- a study in the Norwegian Mother and Child Cohort Study.

    Science.gov (United States)

    Farbu, Jorunn; Haugen, Margaretha; Meltzer, Helle Margrete; Brantsæter, Anne Lise

    2014-12-05

    Little attention has been given to the impact of singlehood during pregnancy. The aim of this study was to examine the impact of marital status on diet during pregnancy and pregnancy outcome. The study population comprised 62,773 women participating in the Norwegian Mother and Child Cohort Study. Marital status was categorised into singles living alone, singles living with parents and married/cohabiting (reference group). Participants answered a general health questionnaire in gestational week 15-17 and a food frequency questionnaire in gestational week 22. We used nonparametric tests to compare dietary intakes by marital status, and multiple logistic regression to estimate odds ratios (OR) and 95% confidence intervals (CI) for infants being small for gestational age (SGA), large for gestational age (LGA), and preterm delivery (defined as delivery before gestational week 37). Single women living with parents had lower intakes of fruits and vegetables, higher intake of total energy, higher proportion of energy from added sugar, and lower intake of fibre than the reference group. Singles living alone also had a higher intake of added sugar. In both of the single groups, daily smoking was more prevalent than in women living with a partner. In analyses adjusted for maternal age, pre-pregnancy BMI, energy intake, energy contributed by protein, education, income, parity and nausea, single women living alone had increased risk of SGA with OR = 1.27 (95% CI: 1.05, 1.55). When smoking was included among the confounding variables, the association was no longer significant. Likewise, singles living alone had increased risk of preterm delivery, with OR = 1.32 (95% CI: 1.01, 1.72) in a partly adjusted model, but the association did not remain significant in a model fully adjusted for confounding variables. Single mothers had lower dietary quality and included more smokers than women who lived with a partner. Single mothers living alone had higher prevalence of SGA and preterm

  2. Adult-to-adult living donor liver transplantation preoperative survey using MDCT, a single medical center experience in Taiwan

    Directory of Open Access Journals (Sweden)

    Te-Cheng Su

    2018-02-01

    Full Text Available This study evaluated multi-detector computed tomography (MDCT scans performed on potential living donors for adult-to-adult liver transplantation (LDLT, with the aim of identifying significant findings that could be used to exclude potential transplantation donors. We retrospectively reviewed the medical records of 151 consecutive potential adult donors for LDLT from May 2007 to January 2015. Liver parenchyma steatosis, focal hepatic mass or intraabdominal malignancy, vascular variations, and donor liver volume were evaluated via MDCT. Grounds for excluding potential donors were also recorded and analyzed. Of the 151 potential donors, nine (6.0% had moderate to severe fatty liver, 37 (24.5% had hepatic arterial variants, 22 (14.6% had significant portal venous variants, and more than half were found to have right accessory inferior hepatic vein. No intraabdominal malignancies were found. Eighty-eight potential donors were rejected, with the most common cause being insufficient recipient volume or remnant donor volume (47.7%, moderate to severe parenchymal steatosis (10.2%, and recipient expiration prior to transplantation (8.0%. An additional 16 potential donors were excluded by the surgical team due to the complexity of their portal venous variations. The rate of exclusion by pre-transplant imaging evaluation with MDCT was 33.8%. MDCT can provide accurate quantification of donor liver volume and steatosis severity along with precise demonstration of vascular variants, which are crucial for the preoperative evaluation of LDLT. However, MDCT may be ineffective for evaluating the biliary system without hepatobiliary-excreted contrast agent and has the disadvantage of ionizing radiation.

  3. The Intersectionality of African American Mothers in Counselor Education: A Phenomenological Examination

    Science.gov (United States)

    Haskins, Natoya H.; Ziomek-Daigle, Jolie; Sewell, Cheryl; Crumb, Lonika; Appling, Brandee; Trepal, Heather

    2016-01-01

    Using phenomenological inquiry, this study explored the lived experiences and intersecting identities of 8 African American counselor educators who are mothers. Six themes were identified: race, professional strain, work-life balance, support, internalized success, and mothering pedagogy.

  4. Contemporary impingements on mothering.

    Science.gov (United States)

    Tummala-Narra, Pratyusha

    2009-03-01

    Mothering in contemporary Western society needs to be understood in the context of a rapidly changing social context. Increased geographic mobility, improved access to child-related information through the media, and scientific and technological progress have contributed to significant shifts in cultural views on mothering. Several contextual impingements on mothering, including changing family structure, economic pressures, decreased social support, cultural ideals of the perfect mother, and increased awareness of interpersonal and global trauma impact mothers' internal worlds. These societal changes often reinforce mothers' fear of losing their children and an idealization of intensive mothering, and evoke challenges in reorganizing their sense of personal identity. Implications for psychoanalytic theory and practice, and specifically the need to integrate individual and contextual forces related to experiences of mothers will be explored.

  5. Unmarried Mother's Knowledge and Attitudes toward Emergency Contraceptive Pills

    Directory of Open Access Journals (Sweden)

    Gyeong Mi Lee

    2013-06-01

    Full Text Available PurposeThis study was conducted to identify relationships among knowledge and attitudes of unmarried mothers toward emergency contraceptive pills.MethodsData were collected through structured questionnaires from 135 unmarried mothers enrolled in 7 single mothers' facilities nationwide. Data were analyzed using the SPSS/WIN 17.0 program for descriptive statistics, t-test, ANOVA, Scheffe-test, and Pearson correlation coefficients.ResultsFor knowledge about emergency contraceptive pills, there were significant differences among who live with her before pregnancy, experience of past pregnancies, state of present pregnancy and preparation in using contraceptives. For attitude toward emergency contraceptive pills, there were significant differences according to age, education level and religion. There were significant positive relationships between knowledge and attitudes toward emergency contraceptive pills.ConclusionThe results of this study suggest that unmarried women should be better informed about emergency contraceptive pills, and reassured about their safety. Efforts are needed to disseminate up-to-date information to experts in sex education including nurses.

  6. Amygdala Response to Mother

    Science.gov (United States)

    Tottenham, Nim; Shapiro, Mor; Telzer, Eva H.; Humphreys, Kathryn L.

    2012-01-01

    In altricial species, like the human, the caregiver, very often the mother, is one of the most potent stimuli during development. The distinction between mothers and other adults is learned early in life and results in numerous behaviors in the child, most notably mother-approach and stranger wariness. The current study examined the influence of…

  7. Unattached fraction of short-lived Rn decay products in indoor and outdoor environments: An improved single-screen method and results

    International Nuclear Information System (INIS)

    Reineking, A.; Porstendoerfer, J.

    1990-01-01

    The unattached fraction fp of potential alpha energy of short-lived Rn decay products was measured under realistic, natural conditions in different dwellings and in the open atmosphere by a single-screen technique. An improved data evaluation method was developed where the measured activities of 218 Po (RaA) and 214 Pb (RaB) were corrected by the screen-attached activities of 214 Bi ( 214 Po) [RaC (RaC')]. This method is based on the experimental observation that the 214 Bi ( 214 Po) unattached activities are negligible under realistic living conditions and that the size distributions of the aerosol-attached activities of all short-lived Rn daughters are identical. In closed rooms without additional aerosol sources, a mean unattached fraction fp of the potential alpha energy of 0.096 was obtained at a mean aerosol particle concentration of 6100 cm-3 and at a mean equilibrium factor F of 0.30. This mean fp value is about three times higher than the value used in the literature for the radiation exposure calculation of the human public. In closed rooms with additional aerosol sources (cigarette smoke, heating systems, aerosols from a burning candle), the aerosol particle concentrations ranged up to 10(6) cm-3 and the attachment rates, X, increased up to 1000 h-1. The fp values sometimes decreased below the detection limit of 0.005, and the F values increased to as high as 0.77. In the ambient atmosphere in the vicinity of Goettingen, a mean unattached fraction fp of 0.02 and a mean aerosol particle concentration of 3.4 x 10(4) cm-3 were measured at 1 m above the ground. The mean equilibrium factor F was determined to be 0.7.A

  8. Improving the drug delivery characteristics of graphene oxide based polymer nanocomposites through the “one-pot” synthetic approach of single-electron-transfer living radical polymerization

    International Nuclear Information System (INIS)

    Gao, Peng; Liu, Meiying; Tian, Jianwen; Deng, Fengjie; Wang, Ke; Xu, Dazhuang; Liu, Liangji; Zhang, Xiaoyong; Wei, Yen

    2016-01-01

    Graphical abstract: The PEGylated graphene oxides with high water dispersibility, good biocompatibility as well as high drug loading capability were fabricated via “one-pot” SET-LRP. - Highlights: • Surface modification of graphene oxide with polymers. • One-pot single-electron-transfer living radical polymerization. • Improving drug delivery characteristics. • The synthetic approach is rather simple, universal and effective. - Abstract: Graphene oxide (GO) based polymer nanocomposites have attracted extensive research interest recently for their outstanding physicochemical properties and potential applications. However, surface modification of GO with synthetic polymers has demonstrated to be trouble for most polymerization procedures are occurred under non-aqueous solution, which will in turn lead to the restacking of GO. In this work, a facile and efficient “one-pot” strategy has been developed for surface modification of GO with synthetic polymers through single-electron-transfer living radical polymerization (SET-LRP). The GO based polymer nanocomposites were obtained via SET-LRP in aqueous solution using poly(ethylene glycol) methyl ether methacrylate (PEGMA) as the monomer and 11-bromoundecanoic acid as the initiator, which could be effectively adsorbed on GO through hydrophobic interaction. The successful preparation of GO based polymer nanocomposites was confirmed by a series of characterization techniques such as 1 H nuclear magnetic resonance, Fourier transform infrared spectroscopy, thermogravimetric analysis, transmission electron microscopy and X-ray photoelectron spectroscopy. The resultant products exhibit high water disperisibility, excellent biocompatibility and high efficient drug loading capability, making these PEGylated GO nanocomposites promising candidates for biomedical applications.

  9. Improving the drug delivery characteristics of graphene oxide based polymer nanocomposites through the “one-pot” synthetic approach of single-electron-transfer living radical polymerization

    Energy Technology Data Exchange (ETDEWEB)

    Gao, Peng; Liu, Meiying; Tian, Jianwen; Deng, Fengjie [Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031 (China); Wang, Ke [Department of Chemistry and the Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing 100084 (China); Xu, Dazhuang [Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031 (China); Liu, Liangji [Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330006 (China); Zhang, Xiaoyong, E-mail: xiaoyongzhang1980@gmail.com [Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031 (China); Wei, Yen, E-mail: weiyen@tsinghua.edu.cn [Department of Chemistry and the Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing 100084 (China)

    2016-08-15

    Graphical abstract: The PEGylated graphene oxides with high water dispersibility, good biocompatibility as well as high drug loading capability were fabricated via “one-pot” SET-LRP. - Highlights: • Surface modification of graphene oxide with polymers. • One-pot single-electron-transfer living radical polymerization. • Improving drug delivery characteristics. • The synthetic approach is rather simple, universal and effective. - Abstract: Graphene oxide (GO) based polymer nanocomposites have attracted extensive research interest recently for their outstanding physicochemical properties and potential applications. However, surface modification of GO with synthetic polymers has demonstrated to be trouble for most polymerization procedures are occurred under non-aqueous solution, which will in turn lead to the restacking of GO. In this work, a facile and efficient “one-pot” strategy has been developed for surface modification of GO with synthetic polymers through single-electron-transfer living radical polymerization (SET-LRP). The GO based polymer nanocomposites were obtained via SET-LRP in aqueous solution using poly(ethylene glycol) methyl ether methacrylate (PEGMA) as the monomer and 11-bromoundecanoic acid as the initiator, which could be effectively adsorbed on GO through hydrophobic interaction. The successful preparation of GO based polymer nanocomposites was confirmed by a series of characterization techniques such as {sup 1}H nuclear magnetic resonance, Fourier transform infrared spectroscopy, thermogravimetric analysis, transmission electron microscopy and X-ray photoelectron spectroscopy. The resultant products exhibit high water disperisibility, excellent biocompatibility and high efficient drug loading capability, making these PEGylated GO nanocomposites promising candidates for biomedical applications.

  10. A living cell quartz crystal microbalance biosensor for continuous monitoring of cytotoxic responses of macrophages to single-walled carbon nanotubes

    Directory of Open Access Journals (Sweden)

    Marx Kenneth A

    2011-01-01

    Full Text Available Abstract Background Numerous engineered nanomaterials (ENMs exist and new ENMs are being developed. A challenge to nanotoxicology and environmental health and safety is evaluating toxicity of ENMs before they become widely utilized. Cellular assays remain the predominant test platform yet these methods are limited by using discrete time endpoints and reliance on organic dyes, vulnerable to interference from ENMs. Label-free, continuous, rapid response systems with biologically meaningful endpoints are needed. We have developed a device to detect and monitor in real time responses of living cells to ENMs. The device, a living cell quartz crystal microbalance biosensor (QCMB, uses macrophages adherent to a quartz crystal. The communal response of macrophages to treatments is monitored continuously as changes in crystal oscillation frequency (Δf. We report the ability of this QCMB to distinguish benign from toxic exposures and reveal unique kinetic information about cellular responses to varying doses of single-walled carbon nanotubes (SWCNTs. Results We analyzed macrophage responses to additions of Zymosan A, polystyrene beads (PBs (benign substances or SWCNT (3-150 μg/ml in the QCMB over 18 hrs. In parallel, toxicity was monitored over 24/48 hrs using conventional viability assays and histological stains to detect apoptosis. In the QCMB, a stable unchanging oscillation frequency occurred when cells alone, Zymosan A alone, PBs alone or SWCNTs without cells at the highest dose alone were used. With living cells in the QCMB, when Zymosan A, PBs or SWCNTs were added, a significant decrease in frequency occurred from 1-6 hrs. For SWCNTs, this Δf was dose-dependent. From 6-18 hrs, benign substances or low dose SWCNT (3-30 μg/ml treatments showed a reversal of the decrease of oscillation frequency, returning to or exceeding pre-treatment levels. Cell recovery was confirmed in conventional assays. The lag time to see the Δf reversal in QCMB plots

  11. A living cell quartz crystal microbalance biosensor for continuous monitoring of cytotoxic responses of macrophages to single-walled carbon nanotubes

    Science.gov (United States)

    2011-01-01

    Background Numerous engineered nanomaterials (ENMs) exist and new ENMs are being developed. A challenge to nanotoxicology and environmental health and safety is evaluating toxicity of ENMs before they become widely utilized. Cellular assays remain the predominant test platform yet these methods are limited by using discrete time endpoints and reliance on organic dyes, vulnerable to interference from ENMs. Label-free, continuous, rapid response systems with biologically meaningful endpoints are needed. We have developed a device to detect and monitor in real time responses of living cells to ENMs. The device, a living cell quartz crystal microbalance biosensor (QCMB), uses macrophages adherent to a quartz crystal. The communal response of macrophages to treatments is monitored continuously as changes in crystal oscillation frequency (Δf). We report the ability of this QCMB to distinguish benign from toxic exposures and reveal unique kinetic information about cellular responses to varying doses of single-walled carbon nanotubes (SWCNTs). Results We analyzed macrophage responses to additions of Zymosan A, polystyrene beads (PBs) (benign substances) or SWCNT (3-150 μg/ml) in the QCMB over 18 hrs. In parallel, toxicity was monitored over 24/48 hrs using conventional viability assays and histological stains to detect apoptosis. In the QCMB, a stable unchanging oscillation frequency occurred when cells alone, Zymosan A alone, PBs alone or SWCNTs without cells at the highest dose alone were used. With living cells in the QCMB, when Zymosan A, PBs or SWCNTs were added, a significant decrease in frequency occurred from 1-6 hrs. For SWCNTs, this Δf was dose-dependent. From 6-18 hrs, benign substances or low dose SWCNT (3-30 μg/ml) treatments showed a reversal of the decrease of oscillation frequency, returning to or exceeding pre-treatment levels. Cell recovery was confirmed in conventional assays. The lag time to see the Δf reversal in QCMB plots was linearly SWCNT

  12. Implications of Intensive Mothering Beliefs for the Well-Being of Full-Time Employed Mothers of Infants: Moderating Effects of Childcare Satisfaction and Workplace Flexibility

    Science.gov (United States)

    Walls, Jill K.

    2010-01-01

    Hays (1996) was the first to describe the construct of intensive mothering beliefs, yet little empirical research exists to substantiate its prevalence among contemporary mothers and its implications for their lives. The purpose of this study was to examine the construct of intensive mothering beliefs and its longitudinal associations with…

  13. Cellular organization and spectral diversity of GFP-like proteins in live coral cells studied by single and multiphoton imaging and microspectroscopy

    Science.gov (United States)

    Salih, Anya; Cox, Guy C.; Larkum, Anthony W.

    2003-07-01

    Tissues of many marine invertebrates of class Anthozoa contain intensely fluorescent or brightly coloured pigments. These pigments belong to a family of photoactive proteins closely related to Green Fluorescent Protein (GFP), and their emissions range from blue to red wavelengths. The great diversity of these pigments has only recently been realised. To investigate the role of these proteins in corals, we have performed an in vivo fluorescent pigment (FP) spectral and cellular distribution analyses in live coral cells using single and multi-photon laser scanning imaging and microspectroscopy. These analyses revealed that even single colour corals contain spectroscopically heterogeneous pigment mixtures, with 2-5 major colour types in the same area of tissue. They were typically arranged in step-wise light emission energy gradients (e.g. blue, green, yellow, red). The successive overlapping emission-excitation spectral profiles of differently coloured FPs suggested that they were suited for sequential energy coupling. Traces of red FPs (emission = 570-660 nm) were present, even in non-red corals. We confirmed that radiative energy transfer could occur between separate granules of blue and green FPs and that energy transfer was inversely proportional to the square of the distance between them. Multi-photon micro-spectrofluorometric analysis gave significantly improved spectral resolution by restricting FP excitation to a single point in the focal plane of the sample. Pigment heterogeneity at small scales within granules suggested that fluorescence resonance energy transfer (FRET) might be occurring, and we confirmed that this was the case. Thus, energy transfer can take place both radiatively and by FRET, probably functioning in photoprotection by dissipation of excessive solar radiation.

  14. Politics, doctors, assisted reproductive technologies & religion: Transgenerational understandings and experiences of single motherhood in Spain.

    Science.gov (United States)

    Bravo-Moreno, Ana

    2017-10-01

    The aim is to achieve a transgenerational view of single motherhood in Spain, to look at which contexts it arises in, how it changes with the introduction of assisted reproduction, and how the role of religion in Spanish society permeates medical practice and affects the lives of women patients. I examine single motherhood and investigate two interconnected themes: (a) being a mother and being mothered are both permeated with sociocultural, political, religious, economic and psychological significance; (b) Spain led Europe in multiple births due to assisted reproduction, thus ethical conflicts and patient rights are analyzed.

  15. Chronic renal disease: the mother's experience.

    Science.gov (United States)

    MacDonald, H

    1995-01-01

    In a qualitative study, mothers (N = 4) of children with chronic renal disease were asked to share the meaning they assigned to their child's illness. The central theme of "uncertainty" emerged from the data and was described in three phases: (a) finding out, (b) learning to live with chronic illness, and (c) worries and dreams about the future. In this article phase three, labeled "learning to live with chronic illness," is presented.

  16. 9592 THE EXPERIENCES OF HIV-POSITIVE MOTHERS ...

    African Journals Online (AJOL)

    Mimi

    In Swaziland, 41.1% of pregnant women live with HIV, while only 32% of Swazi mothers (including HIV negative mothers) currently practice exclusive breastfeeding among infants less than six months of age. The rate of exclusive breastfeeding decreases with an increase in the infant's age, as only 17% of infants aged four ...

  17. Brazilian Mothers' Socialization Goals: Intracultural Differences in Seven Brazilian Cities

    Science.gov (United States)

    Seidl-de-Moura, Maria Lucia; Lordelo, Eulina; Vieira, Mauro Luis; Piccinini, Cesar Augusto; Siqueira, Jose de Oliveira; Magalhaes, Celina Maria Colino; Pontes, Fernando Augusto Ramos; Salomao, Nadia Maria; Rimoli, Adriana

    2008-01-01

    The present study aimed to investigate Brazilian mothers' socialization goals. The participants in the study were 349 primiparous mothers, whose ages ranged from 17 to 47 years (mean = 26.6 years), who had children aged between 1 and 48 months (mean = 16.4 months). The families were living in seven different cities representing each of the five…

  18. The experiences of HIV-positive mothers breastfeeding exclusively ...

    African Journals Online (AJOL)

    In Swaziland, 41.1% of pregnant women live with HIV, while only 32% of Swazi mothers (including HIV negative mothers) currently practice exclusive breastfeeding among infants less than six months of age. The rate of exclusive breastfeeding decreases with an increase in the infant's age, as only 17% of infants aged four ...

  19. Mothe'rs' Health Services Utilization and Health Care Seeking ...

    African Journals Online (AJOL)

    expenditures for infants during the first year of life. Methods: The study was longitudinal community based study consisted of a cohort of 8273 live births, their mothers and families between September 1992 and October 1994 in rural and urban settings in Jimma, Illubabor and Kefecho Zones, Southwest Ethiopia. Mothers ...

  20. Influence of living status (single vs. paired) and centrifugation with colloids on the sperm morphology and functionality in the clouded leopard (Neofelis nebulosa).

    Science.gov (United States)

    Tipkantha, W; Thuwanut, P; Morrell, J; Comizzoli, P; Chatdarong, K

    2016-12-01

    The objectives of the present study were to evaluate sperm characteristic of captive clouded leopards in Thailand and examine the structural and functional properties of sperm after selection with the single-layer centrifugation (SLC) method. Twenty-two ejaculates from 11 captive clouded leopards (four housed with access to a female in estrus, and seven housed singly) were collected and assessed for semen traits during 2013 to 2015. Twelve fresh ejaculates were chosen from seven males, and each was divided between two sperm preparation methods; (1) simple washing and (2) SLC. Cryopreservation was performed after semen preparation. Sperm qualities after selections including motility, progressive motility, sperm motility index, viability, acrosome integrity, DNA integrity, and morphology were evaluated in fresh, chilled, and frozen-thawed samples. In addition, sperm functionality after cryopreservation was tested by heterologous IVF using domestic cat oocytes. Sperm motility in the ejaculates was 52.5% to 91.3% (76.8 ± 2.0%, mean ± standard error). A high proportion of morphologically abnormal sperm (63.9 ± 2.0%) was observed, with the major abnormality being tightly coiled tail (13.5 ± 0.5%). An interesting observation was that males housed together with a female had a significantly higher proportion of sperm with intact acrosome (47.9 ± 3.4% and 38.4 ± 2.8%) and lower proportion of sperm with bent midpiece and droplet (7.1 ± 0.6% and 10.2 ± 0.5%) than the males living singly. The sperm motility index, intact acrosome, and sperm with normal tail in the fresh and chilled semen samples were improved by the SLC. In the postthawed semen, the SLC selected higher numbers of viable sperm (34.1 ± 2.2% and 27.9 ± 1.8%), sperm with intact acrosome (31.2 ± 2.1% and 24.3 ± 2.2%), and sperm with normal tail (34.2 ± 2.7% and 24.3 ± 2.7%) than simple washing. Also, the proportion of sperm with tightly coiled tail was lower in the SLC

  1. Prayer and meditation among Danish first time mothers

    DEFF Research Database (Denmark)

    Prinds, Christina; Hvidtjørn, Dorte; Skytthe, Axel

    2016-01-01

    among first time mothers conducted during the summer 2011. All Danish women who gave birth before the 32nd pregnancy week (n = 255), and double the number of mothers who gave birth at full term (n = 658) in 2010 were included (total n = 913). The questionnaire consisted of 46 overall items categorized...... or education.ConclusionsIn this explorative study we found specific practices of existential meaning-making through prayer and/or meditation among first time mothers, living in a very secularized context. Yet we know only little about character or importance of these practices among mothers, and hardly...

  2. Teen Mothers' Experience of Intimate Partner Violence: A Metasynthesis.

    Science.gov (United States)

    Bekaert, Sarah; SmithBattle, Lee

    2016-01-01

    Pregnant and parenting teens suffer higher rates of intimate partner violence (IPV) than older mothers. This qualitative metasynthesis explores teen mothers' experience with IPV during pregnancy and postpartum. Organized by the metaphor of a web, findings highlight how pervasive violence during childhood contributes to teen pregnancy and the risk of IPV as violence is normalized. The web constricts through the partner's control as violence emerges or worsens with pregnancy. Young mothers become increasingly isolated, and live with the physical and psychological consequences of IPV. Trauma-informed nursing practice is needed to support teen mothers in violent intimate relationships to spin a new web.

  3. Improving the drug delivery characteristics of graphene oxide based polymer nanocomposites through the "one-pot" synthetic approach of single-electron-transfer living radical polymerization

    Science.gov (United States)

    Gao, Peng; Liu, Meiying; Tian, Jianwen; Deng, Fengjie; Wang, Ke; Xu, Dazhuang; Liu, Liangji; Zhang, Xiaoyong; Wei, Yen

    2016-08-01

    Graphene oxide (GO) based polymer nanocomposites have attracted extensive research interest recently for their outstanding physicochemical properties and potential applications. However, surface modification of GO with synthetic polymers has demonstrated to be trouble for most polymerization procedures are occurred under non-aqueous solution, which will in turn lead to the restacking of GO. In this work, a facile and efficient ;one-pot; strategy has been developed for surface modification of GO with synthetic polymers through single-electron-transfer living radical polymerization (SET-LRP). The GO based polymer nanocomposites were obtained via SET-LRP in aqueous solution using poly(ethylene glycol) methyl ether methacrylate (PEGMA) as the monomer and 11-bromoundecanoic acid as the initiator, which could be effectively adsorbed on GO through hydrophobic interaction. The successful preparation of GO based polymer nanocomposites was confirmed by a series of characterization techniques such as 1H nuclear magnetic resonance, Fourier transform infrared spectroscopy, thermogravimetric analysis, transmission electron microscopy and X-ray photoelectron spectroscopy. The resultant products exhibit high water disperisibility, excellent biocompatibility and high efficient drug loading capability, making these PEGylated GO nanocomposites promising candidates for biomedical applications.

  4. Development of TV003/TV005, a single dose, highly immunogenic live attenuated dengue vaccine; what makes this vaccine different from the Sanofi-Pasteur CYD™ vaccine?

    Science.gov (United States)

    Whitehead, Stephen S

    2016-01-01

    Dengue is caused by four serotype-distinct dengue viruses (DENVs), and developing a multivalent vaccine against dengue has not been straightforward since partial immunity to DENV may predispose to more severe disease upon subsequent DENV infection. The vaccine that is furthest along in development is CYD™, a live attenuated tetravalent vaccine (LATV) produced by Sanofi Pasteur. Although the multi-dose vaccine demonstrated protection against severe dengue, its overall efficacy was limited by DENV serotype, serostatus at vaccination, region and age. The National Institute of Allergy and Infectious Diseases has developed the LATV dengue vaccines TV003/TV005. A single dose of either TV003 or TV005 induced seroconversion to four DENV serotypes in 74-92% (TV003) and 90% (TV005) of flavivirus seronegative adults and elicited near-sterilizing immunity to a second dose of vaccine administered 6-12 months later. The important differences in the structure, infectivity and immune responses to TV003/TV005 are compared with CYD™.

  5. Striatal and extrastriatal imaging of dopamine D2receptors in the living human brain with [ 123I[epidepride single-photon emission tomography

    International Nuclear Information System (INIS)

    Kuikka, J.T.; Aakerman, K.K.; Hiltunen, J.; Bergstroem, K.A.; Raesaenen, P.; Vanninen, E.; Halldin, C.; Tiihonen, J.

    1997-01-01

    The iodine-123 labelled ligand benzamide epidepride was evaluated as a probe for in vivo imaging of striatal and extrastriatal dopamine D 2 receptor sites in the human brain. Four healthy males were imaged with a high-resolution single-photon emission tomography scanner. Striatal radioactivity peaked at 3 h after injection. The specific binding in the striatum was 0.91 ±0.03 at 3 h and this ratio steadily increased with time. Extrastriatal radioactivity was highest in the thalamus, in the midbrain and in the temporal cortex, and peaked at 45-60 min after injection of tracer. A smaller amount of radioactivity was found in the parietal, frontal and occipital cortices. Two radioactive metabolites were observed, of which one was more lipophilic than the parent compound. The radiation burden to the patient was 0.035 mSv/MBq (effective dose equivalent). The preliminary results showed that [ 123 I[epidepride can be used for imaging striatal and extrastriatal dopamine D 2 receptor sites in the living human brain. (orig.). With 5 figs., 1 tab

  6. Comparison of Irrational Believes between Mothers of Severe or Profound Mentally Handicapped Children with Healthy Children Mothers

    Directory of Open Access Journals (Sweden)

    Behrouz Hivadi

    2007-10-01

    Full Text Available Objective: The purpose of present research was the comparison of mothers irrational believes with severe or profound mentally handicapped child and mothers with normal child from 6 to14 years old in Tehran city. Materials & Methods: This study was an analytical, cross – sectional and comparative (case – control research. From mothers with severe or profound mentally handicapped child who had refered to Tehran welfare services centers, 80 mothers were selected by regular randomized sampling from two rehabilitation centers and 80 mothers with normal child were selected for peering with the group of testimonial from schools areas of east, west, south, north and center of Tehran, through multi - stage cluster sampling in for variables of: age of mothers, educational levels, the location of living and the number of children. They answered to questionnaire of irrational believes of jons (IBT. Analysis of data was done by descriptive and infringing statistics methods (Independent T test, U Mann Whitney, Chi-square and fisher. Results: The findings showed that: there are significantly differences in total irrational believes and irrational believes of blame proneness, frustration reactive, anxious over concern, problem avoiding and dependency, perfectionism between two groups of mothers (P<0/05. There was no significant difference in irrational believes between mothers who had mental handicap daughter and mothers who had mental handicap son (P=0/314. There was no significantly difference between two groups of mothers in four believes of demand for approval (P=0/737, high-self expectation (P=0/126, emotional irresponsibility (P=0/727, helplessness for change (p=0/283. Conclusion: Irrational believes and many its sub scales. In mothers of severe or profound mental handicap children were more than mothers with normal child. But believes of demand for approval, high self expectation, emotional irresponsibility, helplessness for change in mothers with

  7. Perception of the mothering role of women who live in a context of drugs and violence Percepción del papel materno de mujeres que viven en un contexto de drogas y violencia Percepção do papel materno de mulheres que vivem em um contexto de drogas e violência

    Directory of Open Access Journals (Sweden)

    María Carmen Bernal Roldán

    2005-12-01

    Full Text Available The number of women involved with drugs is increasing, and the same is true for violence against them. Many of these women are mothers. This study aimed to investigate how mothers of children between one month and six years old who are living in a context of drugs and violence and are receiving treatment for drug addiction interpret their experience as mothers. This article is part of a multicenter study in Colombia and Brazil and discusses the results of interviews with 6 Colombian mothers. Focused ethnography was used and the interviews were submitted to latent content analysis. Violence was present in the participants' socio-cultural context and drugs consumption in their family of origin. These aspects possibly induced them to become addicted to drugs and influenced their mothering role. Overall, the mothers expressed ambivalence about their role in that they idealized being mothers but were aware that they were addicted mothers.El número de mujeres implicadas con las drogas está aumentando y e la violencia contra ellas también. Muchas de estas mujeres son madres. El objetivo de este estudio fue conocer la manera como las mujeres que tienen hijos entre un mes y cinco años, viven en un contexto de drogas y violencia, y están recibiendo tratamiento para su drogadicción, interpretan su experiencia como madres; este trabajo individual y multicéntrico entre países, Colombia y Brasil, expone los resultados individuales de Colombia; se entrevistaron seis mujeres. Se usó el método de foco etnográfico y las entrevistas fueron sometidas a análisis de contenido latente. Se encontró presente la violencia desde el contexto sociocultural de las participantes y el consumo de drogas en el entorno de la familia de origen, aspectos que posiblemente indujeron drogadicción en ellas e influyeron en el papel maternal de este grupo de mujeres, quienes lo expresan como ambivalente, entre la idealización del ser madre y el ser madre en la adicci

  8. Teen Mothers' Mental Health.

    Science.gov (United States)

    SmithBattle, Lee; Freed, Patricia

    2016-01-01

    Psychological distress is common in teen mothers. High rates of distress are attributed to teen mothers' childhood adversities and the challenges of parenting in the context of chronic stress, cumulative disadvantage, and limited social support. We describe the prevalence of psychological distress in teen mothers; what is known about its origins and impact on mothers and children; factors that promote teen mothers' mental health and resilience; and the many barriers that make it difficult to obtain traditional mental healthcare. We also briefly review the few studies that test interventions to improve teen mothers' mental health. Because barriers to traditional mental health treatment are ubiquitous and difficult to remedy, the second article in this two-part series calls for nurses in healthcare settings, schools, and home visiting programs to screen pregnant and parenting teens for adverse childhood experiences and psychological distress, and to integrate strength-based and trauma-based principles into their practice. Creating a supportive setting where past traumas and psychological distress are addressed with skill and sensitivity builds upon teen mothers' strengths and their aspirations to be the best parents they can be. These approaches facilitate the long-term health and development of mother and child.

  9. Like mother, like daughter

    Indian Academy of Sciences (India)

    Lawrence

    LIKE MOTHER, LIKE DAUGHTER. 309 balancing her dual identities as a scientist and a mother. I did not appreciate these issues until much later when I faced gender based discrimination myself. One great source of inspiration during my. PhD. years was Rafael Sorkin, with whom I worked on a paper on quantum diffusion.

  10. Effects of home-based play-assisted stimulation on developmental performances of children living in extreme poverty: a randomized single-blind controlled trial.

    Science.gov (United States)

    Worku, Berhanu Nigussie; Abessa, Teklu Gemechu; Wondafrash, Mekitie; Lemmens, Johan; Valy, Jan; Bruckers, Liesbeth; Kolsteren, Patrick; Granitzer, Marita

    2018-02-05

    Children living with foster families in a resource-limited setting such as Ethiopia are at risk of developmental problems. It is not yet clear whether intensive home-based developmental stimulation assisted by play can reduce these problems. The main objective of this study was to examine the effects of play-assisted intervention integrated into basic services on the developmental performance of children living with foster families in extreme poverty. A randomized single-blind (investigator) controlled trial design was used. The study was conducted in Jimma, South West Ethiopia. Using computer-generated codes, eligible children of 3-59 months in age were randomly allocated to intervention (n = 39) and control (n = 39) groups at a 1:1 ratio. Children in the intervention group received home-based play-assisted stimulation in addition to the basic services provided to children in both groups. The intervention consisted of an hour of play stimulation conducted during a weekly home visit over the course of six months. Personal-social, language, fine and gross motor outcomes were assessed using Denver II-Jimma, and social-emotional outcome was obtained using an adapted Ages and Stages Questionnaire: Social-Emotional (ASQ: SE). Information about sociodemographic characteristics was collected using a structured questionnaire. Anthropometric methods were used to determine nutritional status. The effects of the intervention on the abovementioned outcomes over the study period and group differences in change over time were examined using Generalized Estimating Equations (GEE). Statistically significant intervention effects were found for language (P = 0.0014), personal-social (P = 0.0087) and social-emotional (P size = 0.34) and social-emotional (effect size = - 0.603) benefits from the play-assisted stimulation had already been observed for the children in the intervention group. For language, the intervention effect depended on the child's sex

  11. Understanding childhood asthma in focus groups: perspectives from mothers of different ethnic backgrounds

    Directory of Open Access Journals (Sweden)

    McKenzie Sheila

    2001-09-01

    Full Text Available Abstract Background Diagnosing childhood asthma is dependent upon parental symptom reporting but there are problems in the use of words and terms. The purpose of this study was to describe and compare understandings of childhood 'asthma' by mothers from three different ethnic backgrounds who have no personal experience of diagnosing asthma. A better understanding of parents' perceptions of an illness by clinicians should improve communication and management of the illness. Method Sixty-six mothers living in east London describing their ethnic backgrounds as Bangladeshi, white English and black Caribbean were recruited to 9 focus groups. Discussion was semi-structured. Three sessions were conducted with each ethnic group. Mothers were shown a video clip of a boy with audible wheeze and cough and then addressed 6 questions. Sessions were recorded and transcribed verbatim. Responses were compared within and between ethnic groups. Results Each session, and ethnic group overall, developed a particular orientation to the discussion. Some mothers described the problem using single signs, while others imitated the sound or made comparisons to other illnesses. Hereditary factors were recognised by some, although all groups were concerned with environmental triggers. Responses about what to do included 'normal illness' strategies, use of health services and calls for complementary treatment. All groups were concerned about using medication every day. Expectations about the quality of life were varied, with recognition that restrictions may be based on parental beliefs about asthma, rather than asthma itself. Conclusion Information from these focus groups suggests mothers know a great deal about childhood asthma even though they have no personal experience of it. Knowledge of how mothers from these ethnic backgrounds perceive asthma may facilitate doctor – patient communication with parents of children experiencing breathing difficulties.

  12. Mothering differently: narratives of mothers with intellectual disability whose children have been compulsorily removed.

    Science.gov (United States)

    Mayes, Rachel; Llewellyn, Gwynnyth

    2012-06-01

    Despite the frequency with which mothers with intellectual disability have their children removed, little theoretical or empirical work has understood the mothers' perspectives on this. A few studies have reported mothers' feelings of grief and loss and their sense of powerlessness in the child protection system. This qualitative study explores the daily life narratives of 7 mothers with intellectual disability following the involuntarily removal of their children. For most mothers, having a child removed was not a one-off experience. The serial nature of the experience yielded 3 different narratives, lived out in different ways. In some cases, women told a different narrative for each of their removed children. All women remained focused on their children in care. The multiple and varied narratives of mothers with intellectual disability who have children in care suggest that their support needs may differ from each other and over time. How their support needs might best be met remains an unanswered question. Further research is also needed to identify any adverse health and social consequences for mothers with children in care as well as the effects on their children.

  13. African-American homeless and low-income housed mothers: comparison of parenting practices.

    Science.gov (United States)

    Koblinsky, S A; Morgan, K M; Anderson, E A

    1997-01-01

    The child-rearing practices of homeless and low-income housed mothers of preschool children in Head Start were compared. Overall, homeless mothers provided less learning and academic stimulation, less variety in social and cultural experiences, less warmth and affection, and a less positive physical environment for their children than did housed mothers. Mothers in both living arrangements provided more language stimulation to daughters than to sons. Implications of the findings for working with homeless families are discussed.

  14. The health and socio-economic circumstances of British lone mothers over the last two decades

    DEFF Research Database (Denmark)

    Shouls, S; Whitehead, M; Burström, B

    1999-01-01

    This article examines the trends in the socio-economic circumstances and health of lone mothers compared to couple mothers from 1979 to 1995 in Great Britain using secondary analysis of data from General Household Survey and covering 9,159 lone mothers and 51,922 couple mothers living in private...... households. The main measures are self perceived general health, limiting long-standing illness, poverty and working status....

  15. Knowledge and belief of nursing mothers on nutritional ...

    African Journals Online (AJOL)

    This study was designed to determine the knowledge, attitudes and practices of nursing mothers with respect to food restriction during acute diarrhoea in Ibadan, South West Nigeria. This single round cross-sectional study adopted a purposive sampling procedure to select 250 nursing mothers aged between 17 and 45 ...

  16. Which factors are most predictive for live birth after in vitro fertilization and intracytoplasmic sperm injection (IVF/ICSI) treatments? Analysis of 100 prospectively recorded variables in 8,400 IVF/ICSI single-embryo transfers.

    Science.gov (United States)

    Vaegter, Katarina Kebbon; Lakic, Tatevik Ghukasyan; Olovsson, Matts; Berglund, Lars; Brodin, Thomas; Holte, Jan

    2017-03-01

    To construct a prediction model for live birth after in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) treatment and single-embryo transfer (SET) after 2 days of embryo culture. Prospective observational cohort study. University-affiliated private infertility center. SET in 8,451 IVF/ICSI treatments in 5,699 unselected consecutive couples during 1999-2014. A total of 100 basal patient characteristics and treatment data were analyzed for associations with live birth after IVF/ICSI (adjusted for repeated treatments) and subsequently combined for prediction model construction. Live birth rate (LBR) and performance of live birth prediction model. Embryo score, treatment history, ovarian sensitivity index (OSI; number of oocytes/total dose of FSH administered), female age, infertility cause, endometrial thickness, and female height were all independent predictors of live birth. A prediction model (training data set; n = 5,722) based on these variables showed moderate discrimination, but predicted LBR with high accuracy in subgroups of patients, with LBR estimates ranging from 40%. Outcomes were similar in an internal validation data set (n = 2,460). Based on 100 variables prospectively recorded during a 15-year period, a model for live birth prediction after strict SET was constructed and showed excellent calibration in internal validation. For the first time, female height qualified as a predictor of live birth after IVF/ICSI. Copyright © 2016 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  17. Needs of Children Experiencing Homelessness who are Living in Shelters: A Qualitative Investigation of Perceptions of Care Workers to Inform Music Therapy Clinical Practice

    OpenAIRE

    Greta Jean Yates; Michael Joseph Silverman

    2016-01-01

    On a single night in January in 2014 there were 194,000 children living with their families in shelters in the United States (National Center on Family Homelessness, 2015). A typical family experiencing homelessness consists of a single mother with two to three children. Children experiencing homelessness are more likely to face academic, social, and emotional problems compared to children in poverty. As there is currently a dearth of peer reviewed publications related to music therapy with c...

  18. Live-cell time-lapse imaging and single-cell tracking of in vitro cultured neural stem cells - Tools for analyzing dynamics of cell cycle, migration, and lineage selection.

    Science.gov (United States)

    Piltti, Katja M; Cummings, Brian J; Carta, Krystal; Manughian-Peter, Ayla; Worne, Colleen L; Singh, Kulbir; Ong, Danier; Maksymyuk, Yuriy; Khine, Michelle; Anderson, Aileen J

    2018-01-15

    Neural stem cell (NSC) cultures have been considered technically challenging for time-lapse analysis due to high motility, photosensitivity, and growth at confluent densities. We have tested feasibility of long-term live-cell time-lapse analysis for NSC migration and differentiation studies. Here, we describe a method to study the dynamics of cell cycle, migration, and lineage selection in cultured multipotent mouse or human NSCs using single-cell tracking during a long-term, 7-14 day live-cell time-lapse analysis. We used in-house made PDMS inserts with five microwells on a glass coverslip petri-dish to constrain NSC into the area of acquisition during long-term live-cell imaging. In parallel, we have defined image acquisition settings for single-cell tracking of cell cycle dynamics using Fucci-reporter mouse NSC for 7 days as well as lineage selection and migration using human NSC for 14 days. Overall, we show that adjustments of live-cell analysis settings can extend the time period of single-cell tracking in mouse or human NSC from 24-72 h up to 7-14 days and potentially longer. However, we emphasize that experimental use of repeated fluorescence imaging will require careful consideration of controls during acquisition and analysis. Copyright © 2017. Published by Elsevier Inc.

  19. Reintegration of young mothers

    Directory of Open Access Journals (Sweden)

    Miranda Worthen

    2012-08-01

    Full Text Available Young mothers seeking reintegration after periods of time spent livingwith fighting forces and armed groups face exclusion and stigmarather than the support they and their children badly need.

  20. Mothers with intellectual disabilities

    OpenAIRE

    Kolarič, Sandra

    2015-01-01

    For the theoretical part of this master's thesis foreign literature and finished foreign researches were studied. In this part of the thesis the characteristics of mothers with intellectual disabilities; factors, which influence the success of carrying out their mother role; and the rights of people with intellectual disabilities as parents, all based on Slovene legislation are included. We listed reasons for limiting reproduction for women with intellectual disabilities and issues concerning...

  1. Single cell analysis of yeast replicative aging using a new generation of microfluidic device.

    Directory of Open Access Journals (Sweden)

    Yi Zhang

    Full Text Available A major limitation to yeast aging study has been the inability to track mother cells and observe molecular markers during the aging process. The traditional lifespan assay relies on manual micro-manipulation to remove daughter cells from the mother, which is laborious, time consuming, and does not allow long term tracking with high resolution microscopy. Recently, we have developed a microfluidic system capable of retaining mother cells in the microfluidic chambers while removing daughter cells automatically, making it possible to observe fluorescent reporters in single cells throughout their lifespan. Here we report the development of a new generation of microfluidic device that overcomes several limitations of the previous system, making it easier to fabricate and operate, and allowing functions not possible with the previous design. The basic unit of the device consists of microfluidic channels with pensile columns that can physically trap the mother cells while allowing the removal of daughter cells automatically by the flow of the fresh media. The whole microfluidic device contains multiple independent units operating in parallel, allowing simultaneous analysis of multiple strains. Using this system, we have reproduced the lifespan curves for the known long and short-lived mutants, demonstrating the power of the device for automated lifespan measurement. Following fluorescent reporters in single mother cells throughout their lifespan, we discovered a surprising change of expression of the translation elongation factor TEF2 during aging, suggesting altered translational control in aged mother cells. Utilizing the capability of the new device to trap mother-daughter pairs, we analyzed mother-daughter inheritance and found age dependent asymmetric partitioning of a general stress response reporter between mother and daughter cells.

  2. Immunogenicity and protective efficacy of a single-dose live non-pathogenic Escherichia coli oral vaccine against F4-positive enterotoxigenic Escherichia coli challenge in pigs.

    Science.gov (United States)

    Fairbrother, John Morris; Nadeau, Éric; Bélanger, Louise; Tremblay, Cindy-Love; Tremblay, Danielle; Brunelle, Mélanie; Wolf, Regina; Hellmann, Klaus; Hidalgo, Álvaro

    2017-01-05

    Enterotoxigenic Escherichia coli strains expressing F4 (K88) fimbriae (F4-ETEC) are one of the most important causes of post-weaning diarrhea (PWD) in pigs. F4, a major antigen, plays an important role in the early steps of the infection. Herein, the efficacy of a live oral vaccine consisting of a non-pathogenic E. coli strain expressing F4 for protection of pigs against PWD was evaluated. Three blinded, placebo-controlled, block design, parallel-group confirmatory experiments were conducted, using an F4-ETEC PWD challenge model, each with a different vaccination-challenge interval (3, 7, and 21days). The pigs were vaccinated via the drinking water with a single dose of the Coliprotec® F4 vaccine one day post-weaning. Efficacy was assessed by evaluating diarrhea, clinical observations, intestinal fluid accumulation, weight gain, intestinal colonization and fecal shedding of F4-ETEC. The immune response was evaluated by measuring serum and intestinal F4-specific antibodies. The administration of the vaccine resulted in a significant reduction of the incidence of moderate to severe diarrhea, ileal colonization by F4-ETEC, and fecal shedding of F4-ETEC after the heterologous challenge at 7 and 21days post-vaccination. The 7-day onset of protection was associated with an increase of serum anti-F4 IgM whereas the 21-day duration of protection was associated with an increase of both serum anti-F4 IgM and IgA. Significant correlations between levels of serum and intestinal secretory anti-F4 antibodies were detected. Maternally derived F4-specific serum antibodies did not interfere with the vaccine efficacy. The evaluation of protection following a challenge three days after vaccination showed a reduction of the severity and the duration of diarrhea and of fecal shedding of F4-ETEC. The 7-day onset and the 21-day duration of protection induced by Coliprotec® F4 vaccine administered once in drinking water to pigs of at least 18days of age were confirmed by protection

  3. After the Baby: Work-Family Conflict and Working Mothers' Psychological Health

    Science.gov (United States)

    Marshall, Nancy L.; Tracy, Allison J.

    2009-01-01

    This study examines work and family characteristics and depressive symptomatology among over 700 working mothers of infants. Working mothers in poorer quality jobs, as well as working mothers who were single or whose infant's health was poorer than that of other infants, reported greater depressive symptomatology. The effect of job quality on…

  4. Stigma and Discrimination: Perspectives from Mexican and Puerto Rican Mothers of Children with Special Needs

    Science.gov (United States)

    McHatton, Patricia Alvarez; Correa, Vivian

    2005-01-01

    The purpose of this study was to investigate the experiences of stigma-related discrimination in 50 Puerto Rican and Mexican single mothers of young children with disabilities. Mothers were interviewed on issues related to raising a child with disabilities. This was a preliminary exploration of the stories mothers told specifically related to…

  5. Living with Stepparents

    Science.gov (United States)

    ... Sometimes spouses die and husbands or wives are forced to start over. Despite all the pain of ... on this topic for: Kids Kids Talk About: Marriage and Divorce (Video) Living With a Single Parent ...

  6. Health Seeking Behavior among Mothers of Sick Children.

    Science.gov (United States)

    Shrestha, P D

    2015-01-01

    Infant and under-five mortality rate in Nepal are 46 and 54 deaths per 1,000 live births, respectively. These mortality indicates, one in every 22 Nepalese children dies before reaching age 1, and one in every 19 does not survive to his or her fifth birthday. Delay in seeking appropriate care and not seeking any care contributes to the large number of child deaths. Existing interventions could prevent many deaths among children if they are presented at health facility and timely care. A descriptive research was carried out in Lele VDC, ward no.7, Lalitpur. The objective of this study was to find out health seeking behavior among mothers of sick children. Non probability, purposive sampling method was used. Sample size was 102 mothers who had sick children from 0 to 59 months. A set of semi structured questionnaire was used to obtain the data. The mean age of the respondent was 25.8 years and child was 29 months. Respondents' children who suffered with pneumonia, diarrhoea and malnutrition were 64(62.7%),29(28%), 9(8.8%) respectively. Majority 84(81.4%) mothers had sought treatment and among them 58(69%) sought treatment from health facility whereas 26(31%) sought treatment from traditional healer. There was significant relationship between education of the mother(p=0.05), sex of the child (p=0.004), type of sickness of children (p=0.001) of the mother and health seeking behaviour of mothers. However occupation of the mothers for seeking treatment (p=0.66) and treatment seeking at first (p=0.82) were not significant. So there was no relationship between occupation of the mothers and health seeking behaviour. Majority of the mothers sought treatment from health facility, yet around one fourth went at traditional healers. Education of the mother, sex of the child, sickness of child and mother's awareness are the factors affecting health seeking behavior of the mothers.

  7. A vivência da maternidade de mães de crianças com autismo La vivencia de la maternidad en madres de niños con autismo The living of maternity with autist children mothers

    Directory of Open Access Journals (Sweden)

    Luciane Najar Smeha

    2011-03-01

    con diagnóstico de autismo.The study aimed at understanding how mothers of children with autism experience motherhood. Participants included four women with children who have autism diagnosis. Data were obtained through semi-structured interviews and analyzed individually as Qualitative Textual Analysis. The results point out that this experience is a challenging experience. These women will not pursue careers, social life and personal relationships in favor of maternal care. Some feelings arise with such uncertainty, sadness and helplessness. We conclude that it is necessary to enable these mothers a space where they can be heard, share experiences and relieve their distress. Finally it is emphasized that psychology can work as a network of support to these women and contribute to the prevention of mental health in families that have a component with a diagnosis of autism.

  8. HIV POSITIVE MOTHERS; ISSUES AND CONCERNS: A QUALITATIVE STUDY

    OpenAIRE

    Manjunatha; Revathi Devi; Arpitha; Chandrakumar

    2014-01-01

    OBJECTIVES: To get an insight into: 1. Perceived health needs and demands of positive mothers. 2. Constraints and barriers in accessing health services. 3. Impact of positive status on social and economic entitlements.4. Role of positive network in educating and empowering mothers. METHODOLOGY: Type of study; Qualitative research using focus group discussions (FGD). Study Setting; “AnandaJoythi Network of People Living with HIV/AIDS®”- A positive network at Mysore. Period ...

  9. Patterns of partnership formation among lone mothers in Russia

    OpenAIRE

    Cordula Zabel

    2008-01-01

    This study examines the determinants of partnership formation among lone mothers in Russia, using data from the Russian Generations and Gender Survey (GGS) and the Education and Employment Survey (EES). The central research question is whether difficult economic circumstances pressure lone mothers to enter new partnerships sooner than they would under other circumstances, limiting their freedom of choice of type of living arrangement. The empirical results show that while occupation influence...

  10. A Proposed Classification System and Therapeutic Strategy for Chyle Leak After Laparoscopic Living-Donor Nephrectomy: A Single-Center Experience and Review of the Literature.

    Science.gov (United States)

    Ng, Zi Qin; He, Bulang

    2018-04-01

    Chyle leak or chylous ascites remains a rare complication after laparoscopic living-donor nephrectomy. Its cause and management have not been well elucidated in the literature. Thus, the aim of this study was to review the incidence of chyle leak/chylous ascites after laparoscopic living-donor nephrectomy in our institute and in the literature to propose a classification system with its associated treatment strategy. In this retrospective review of laparoscopic living-donor nephrectomy patients from January 2005 to April 2016, we identified patients with chyle leak/chylous ascites along with the care performed. A proposed classification system based on our experience and literature is described. Chylous leak developed in 4 donors (2.25%). Of the 4 donors, 3 were treated nonoperatively with diet modification and subcutaneous octreotide injection. One patient required surgical intervention after not responding to second-line therapy with total parenteral nutrition. Chyle leak/chylous ascites after laparoscopic living-donor nephrectomy is rare, but a delayed diagnosis may lead to morbidity secondary to malnutrition and immunosuppression. Meticulous surgical dissection is essential to seal the lymphatic tubes during laparoscopic living-donor nephrectomy. The proposed classification system provides a practical and tailored guide to management based on the drainage volume of chyle leak and a guide to the earlier identification of refractory cases.

  11. The Chinese Tiger Mother

    OpenAIRE

    Jacek Hołówka

    2011-01-01

    In 2010 a book by Amy Chua: Battle Hymn of the Tiger Mother was published and it sparked a broad discussion among pedagogues and the open society about the factors determining educational success. Chua forms a simple and provocative thesis – the Chinese mothers are the best in the world because they don’t spoil their children, quickly introduce them into the adult culture, have high expectations of them, they are brusque and cold but they teach their children how to survive and be competitive...

  12. Detection and analysis of human serum albumin nanoparticles within phagocytic cells at the resolution of individual live cell or single 3D multicellular spheroid

    Energy Technology Data Exchange (ETDEWEB)

    Afrimzon, Elena; Zurgil, Naomi; Sobolev, Maria; Shafran, Yana [Bar-Ilan University, The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome (Israel); Langer, Klaus; Zlatev, Iavor [Westfälischen Wilhelms-Universität Münster, Institut für Pharmazeutische Technologie und Biopharmazie (Germany); Wronski, Robert; Windisch, Manfred [QPS Austria GmbH (Austria); Briesen, Hagen von [Fraunhofer Institute for Biomedical Engineering IBMT, Department of Cell Biology & Applied Virology (Germany); Schmidt, Reinhold [Medical University of Graz, Department of Neurology (Austria); Pietrzik, Claus [University Medical Center of the Johannes Gutenberg University of Mainz, Institute of Pathobiochemistry (Germany); Deutsch, Mordechai, E-mail: motti.jsc@gmail.com [Bar-Ilan University, The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome (Israel)

    2015-12-15

    Since nanoparticles (NPs) have shown great potential in various biomedical applications, live cell response to NPs should be thoroughly explored prior to their in vivo use. In the current study, live cell array (LCA) methodology and unique cell-based assays were used to study the interaction of magnetite (HSA-Mag NP) loaded human serum albumin NPs with phagocytic cells. The LCA enabled cell culturing during HSA-Mag NP accumulation and monolayer or spheroid formation, concomitantly with on-line monitoring of NP internalization. These platforms were also utilized for imaging intercellular links between living cells preloaded with HSA-Mag NP in 2D and 3D resolution. HSA-Mag NP uptake by cells was quantified by imaging, and analyzed using time-resolved measurements. Image analysis of the individual cells in cell populations showed accumulation of HSA-Mag NP by promonocytes and glial cells in a dose- and time-dependent manner. High variability of NP accumulation in individual cells within cell populations, as well as cell subgroups, was evident in both cell types. Following 24 h interaction, uptake of HSA-Mag NP was about 10 times more efficient in glial cells than in activated promonocytes. The presented assays may facilitate detection and analysis of the amount of NPs within individual cells, as well as the rate of NP accumulation and processing in different subsets of living cells. Such data are crucial for estimating predicted drug dosage delivered by NPs, as well as to study possible mechanisms for NP interference with live cells.

  13. Effects of single parenthood on educational aspiration and student disengagement in Korea

    Directory of Open Access Journals (Sweden)

    Hyunjoon Park

    2008-05-01

    Full Text Available The recent rapid increase in divorce, along with its distinctive cultural and welfare environments for single-parent families, makes Korea an interesting case for examining effects of single parenthood on children's education. Using data from Korean 9th and 12th graders, I compare the levels of educational aspiration and student disengagement between students with two parents and those with a single parent, distinguishing divorced single fathers, widowed single fathers, divorced single mothers, and widowed single mothers. Logistic regression analyses show that students with a divorced single parent, regardless of gender of the parent, are much less likely to aspire to four-year university education and more likely to be disengaged than their counterparts with two parents. The effects of widowhood disappear once control variables are held constant. Lower household income among single-parent families explains in part the poorer educational outcomes of their children. Parent-child interaction is another important mediating factor for the effect of single fatherhood but not for single motherhood. The relevance of the extended family system and distinctive features of post-divorce living arrangements in Korea is discussed to understand the effects of single parenthood.

  14. Our Mother Corn.

    Science.gov (United States)

    Mathers, Sherry; And Others

    Developed to provide an understanding of the magnitude of the role of corn, referred to as Mother Corn in the cultures of the Seneca, Pawnee, and Hopi tribes, the student text provides information on the tribes' basic lifestyles and the way they grew and used corn in three different parts of the United States. The section on the origin of corn…

  15. Counselling sessions increased duration of exclusive breastfeeding: a randomized clinical trial with adolescent mothers and grandmothers

    Science.gov (United States)

    2014-01-01

    Background Considering that adolescent mothers may be more vulnerable to discontinuing exclusive breastfeeding (EBF) before 6 months and that their mothers may exert a negative influence on this practice, this study was conducted with the objective of evaluating the efficacy of breastfeeding counselling for adolescent mothers and their mothers in increasing EBF duration. Methods A clinical trial was performed in 323 adolescent mothers with newborns and their mothers randomized in four groups: (1) not living with mother, without intervention; (2) not living with mother, with intervention; (3) living with mother, without intervention, (4) living with mother, with intervention. The intervention consisted of five counselling sessions directed to mother and grandmother, in the maternity hospital and on follow-up. Information about feeding practices during the newborn’s first six months of life was collected monthly by telephone. Intervention’s efficacy was measured through Cox regression and comparison of exclusive breastfeeding medians and survival curves for the different groups. Results The intervention increased the duration of EBF by67 days for the group which included grandmothers (HR = 0.64; CI 95% = 0.46-0.90) and 46 days for the group which did not include grandmothers (HR = 0.52; CI 95% = 0.36-0.76). Conclusions Counselling sessions in the first four months of children’s lives proved to be effective in increasing EBF duration among adolescent mothers. Trial registration ClinicalTrials.gov NCT00910377. PMID:25033743

  16. The experiences of older mothers following the return of an adult child home.

    Science.gov (United States)

    Schwarts, Yehudit; Ayalon, Liat

    2015-04-01

    The present study examines the experience of co-residence of older mothers with their adult children who have returned home, as seen from the mothers' perspective. The population of the study consisted of 14 women between the ages of 58 and 74, whose sons and daughters aged 30 to 40 had come to live with them. The study is a qualitative one, conducted on the basis of in-depth, semi-structured interviews with the mothers. The data were analyzed using constant comparisons. The analysis of the interviews yielded four main themes: a) the mother's perception of the parental role; b) the mother's perception of the returning son or daughter; c) the mother's perception of living together with the adult child; and d) the emotional ramifications arising from co-residence. The differences among the mothers interviewed allowed for the distinction of three types: (1) the mother as rescuer (2) the ambivalent mother and (3) the involved mother. The study sheds light on this late stage of the mother-child relationship, points to the complexity of the phenomenon, and offers insights for professionals working with clients in such situations. Copyright © 2015 Elsevier Inc. All rights reserved.

  17. Inventing atomic resolution scanning dielectric microscopy to see a single protein complex operation live at resonance in a neuron without touching or adulterating the cell.

    Science.gov (United States)

    Agrawal, Lokesh; Sahu, Satyajit; Ghosh, Subrata; Shiga, Takashi; Fujita, Daisuke; Bandyopadhyay, Anirban

    2016-12-01

    A substantial ion flow in a normally wet protein masks any other forms of signal transmission. We use hysteresis and linear conduction (both are artifacts) as a marker to precisely wet a protein, which restricts the ionic conduction (hysteresis disappears), and at the same time, it is not denatured (quantized conductance and Raman spectra are intact). Pure electric visualization of proteins at work by eliminating the screening of ions, electrons, would change the way we study biology. Here we discuss the technical challenges resolved for imaging a protein or live cell using nonlinear dielectric response (spatial distribution of conductance, capacitance and phase, GCP trio). We electromagnetically triggered electrical, mechanical, thermal and ionic resonant vibrations in a protein. During resonant oscillations, we imaged the protein using resonant scanning tunneling microscopy of biomaterials (Brestum) and during ionic firing we imaged live what happens inside an axon core of a neuron by using our atomic scale scanning dielectric microscopy (Asadim). Both Asadim and Brestum are housed in a homebuilt scanning tunneling microscope (bio-STM) and a special micro-grid developed by us (patent JP-5187804) for fractal supercomputing. We found the trick to turn a membrane transparent and see inside without making any physical contact. We image live that a protein molecule adopts a unique configuration for each resonance frequency, - thus far unknown to biology. "Membrane alone fires" is found to be wrong after a century, micro-neuro-filaments communicate prior to firing to decide its necessity and then regulate it suitably. We introduce a series of technologies e.g., fractal grid, point contact, micro THz antenna, to discover that from atomic structure to a living cell, the biomaterials vibrate collectively.

  18. Mental health among single and partnered parents in South Korea.

    Directory of Open Access Journals (Sweden)

    Kyoung Ae Kong

    Full Text Available This study compares the mental health of single parents relative to partnered parents and assesses the contribution of the social and demographic factors to this difference, examining the gender difference in it.We analyzed 12,024 single and partnered subjects, aged 30-59 years, living with children, aged 0-19 years, drawn from the 4th, 5th, and 6th Korea National Health and Nutrition Examination Survey (KNHANES dataset in South Korea conducted from 2007-2013. Mental health was evaluated by self-reported questionnaires including depressive mood for recent two weeks, presence of suicidal ideation, and the Korean version of the Alcohol Use Disorder Identification Test. Covariates included age, physical illness, socioeconomic status (family income, recipient of national basic livelihood guarantees, educational level, house ownership, job, and residential area, family structure, and support (co-residence of another adult. Multiple logistic regression was carried out and the explained fractions of each covariate was calculated.Single parents had significantly poorer mental health than their partnered counterparts, with odds ratio (OR of 2.02 (95% confidence interval (CI 1.56-2.63 for depressive symptoms, 1.69 (95% CI 1.27-2.25 for suicidal ideation, and 1.74 (95% CI 1.38-2.20 for any of the three mental health statuses (suspicious depression, suicidal ideation, and alcohol dependence after controlling for the covariates. The odds of depressive symptoms (OR = 3.13, 95% CI 2.50-3.93 and suicidal ideation (OR = 2.50, 95% CI 1.97-3.17 among both single fathers and mothers were higher than partnered parents. However, the odds of alcohol dependence were 3.6 times higher among single mothers than partnered mothers (OR = 3.58, 95% CI 1.81-7.08 and were 1.4 times greater among single fathers than partnered fathers (OR = 1.35, 95% CI 0.81-2.25. Socio-economic status explained more than 50% (except for substance use disorders of the poorer mental health in

  19. The impact of a breast cancer diagnosis in young women on their relationship with their mothers.

    Science.gov (United States)

    Ali, Asma; Fergus, Karen; Wright, Frances C; Pritchard, Kathleen I; Kiss, Alex; Warner, Ellen

    2014-02-01

    For young women with breast cancer mothers might either be a source of support or of increased stress, depending on the premorbid relationship and on the psychological effect of the daughter's diagnosis on her mother. To examine the effects of a breast cancer diagnosis on young women's relationships with their mothers and the possible support needs of these mothers from the daughters' perspective. We developed and pre-tested a self-administered questionnaire on 10 survivors of breast cancer diagnosed ≤ age 40. Then, consecutive recurrence-free young women diagnosed with breast cancer were asked to complete the modified questionnaire. Of 110 daughters approached from July/11 to May/12, 90 (82%) participated. In 11 cases (13%), the daughters had turned to their mothers before approaching anyone else. Of the 83 daughters who disclosed their diagnosis to their mothers, 76 (92%) stated their mothers had been emotionally and/or practically supportive, and 43 (54%) reported that the breast cancer diagnosis had a favorable impact on their relationship with their mothers. Of the 35 employed mothers, 26 had taken time off from work to support their daughters. Nineteen mothers, eight of whom had been living in a different country, had slept over or moved in with daughters during their treatment. Twenty-two daughters believed their mothers felt responsible to some extent for their developing breast cancer. Fifty-nine daughters indicated that health care professionals could help mothers by providing information pamphlets, education sessions, and linking to support groups. Mothers are an important source of support for young daughters with breast cancer, with the mother-daughter relationship frequently becoming closer after the diagnosis. However, the practical and emotional burden on mothers appears to be high. Future studies should address the effects of a breast cancer diagnosis in young daughters from the mothers' perspective, and the benefit of formal supports for these

  20. Comportamentos e práticas educativas maternas em famílias de mães solteiras e famílias nucleares Comportamientos y prácticas educativas maternas en familias de madres solteras y familias nucleares Maternal behavior and childrearing practices in single-mother families and two-parent families

    Directory of Open Access Journals (Sweden)

    Angela Helena Marin

    2007-04-01

    -Whitney no reveló discrepancias significativas entre las familias para las categorías examinadas. Los resultados sugieren que las configuraciones familiares investigadas no afectan, necesariamente, los comportamientos y las prácticas educativas maternas, ni mucho menos los comportamientos infantiles.Eventual differences in maternal behavior, childrearing practices and children’s behaviors in single-mother and two-parent families are analyzed. Fourteen families, 7 constituted by single mothers (mother and child and 7 by married mothers (mother, father and child, selected in hospitals and health units of Porto Alegre RS Brazil, participated in the study. The families were matched according to age, education and socioeconomic level. Using a protocol with several categories an observation session of the family at lunchtime in the participants’ home, when the children were 30-36 months old, was used to examine the maternal behavior and childrearing practices, as well as the children’s behaviors. Mann-Whitney test indicated no significant differences between single and married mothers for the categories examined. Results suggest that the family structure investigated do not necessarily affect maternal behaviors, childrearing practices and children’s behaviors.

  1. Girl Talk: A Qualitative Study of Girls Talking about the Meaning of Their Lives in an Urban Single-Sex Elementary School

    Science.gov (United States)

    Ridenour, Carolyn S.; Hassell Hughes, Sheila

    2016-01-01

    The suburban-urban achievement gap (diminishing until the 1980s) has stopped its narrowing trend, and single-sex schools are proliferating as a reform model, especially in urban areas. In this study researchers interviewed eight elementary school girls (in an all-girls school) three times over 2 years, and the resulting 23 transcripts were…

  2. Breast milk reduces the risk of illness in children of mothers with cholera

    DEFF Research Database (Denmark)

    Qureshi, Katja; Mølbak, Kåre; Sandström, Anita

    2006-01-01

    BACKGROUND: A protective effect of breastfeeding against cholera has been demonstrated in areas endemic of cholera. To assess the protection offered by breast milk from mothers living in an area that had been free from cholera for 7 years, we investigated mothers with cholera and their children....../mL). CONCLUSIONS: The protective effect of breast milk against cholera is not confined to endemic areas. Lactating mothers with cholera should receive supportive care to continue breastfeeding....

  3. Application of ureterorenoscope and flexible ureterorenoscope lithotripsy in removing calculus from extracorporeal living donor renal graft: a single-center experience.

    Science.gov (United States)

    Lin, Chun-Hua; Zhang, Zuo-Fu; Wang, Jiahui; Yu, Lu-Xin; Wang, Wen-Ting; Shi, Lei; Lin, Xiang-Nan

    2017-11-01

    Here, we reported our clinical application of ureterorenoscope (URS) and flexible URS lithotripsy in stone removal on 10 cases of excised living donor kidney graft. After the extraction of donor kidney by retroperitoneal laparoscopy, the donor graft was perfused with 4 °C HCA solution. Calculus between 2-4 mm were removed intact with lithotomy forceps under direct vision of URS. Larger calculi of >4 mm were fractured with flexible URS combining holmium laser lithotripsy. Fragments of the calculus were extracted with basket extractor and lithotomy forceps. All operations were successful. The operation time was 14-31 min (average 21.2 ± 6.3 min). The kidneys were then transplanted to the recipients using routine procedure. The transplanted kidneys functioned well after transplantation. Gross hematuria resolved 1-4 d after operation (average 2.6 ± 0.9 d). The transplanted kidneys functioned well without early complications such as functional recovery delay and acute graft rejection. The donors and recipients were followed for 12 months. The size of the transplanted kidneys was normal and new stones or urinary obstruction was not seen upon urinary color Doppler ultrasound examination. In conclusion, we believe it is feasible, safe and effective to use URS or flexible URS combining holmium laser lithotripsy on extracorporeal living donor kidney.

  4. Mothers' experiences of coping with adolescent substance abuse: a phenomenological inquiry.

    Science.gov (United States)

    Groenewald, Candice; Bhana, Arvin

    2017-08-01

    Adolescent substance abuse significantly impacts on the lives of mothers. This paper explores the coping responses of mothers whose adolescents have been admitted to treatment for substance abuse. A multiple case study design was adopted and the data were collected through one-on-one interviews facilitated by the Lifegrid. Interpretative phenomenological analysis was used to interrogate the data. Coping emerged as a complex construct in our analysis. The mothers' used problem-focused and emotion-focused coping in different combinations of withdrawing, tolerating and engaged coping responses. The mothers' coping responses were also influenced by individual and relational factors like subjective distress and the mother-adolescent relationship. We argue that research and practice initiatives should consider using a multidimensional perspective of parental coping that recognises the heterogeneity of mothers' coping responses. Impact statement: The current paper offers new insights into the complexities of mothers' coping responses to adolescent substance abuse.

  5. Assisted Living

    Science.gov (United States)

    Assisted living is for adults who need help with everyday tasks. They may need help with dressing, bathing, ... don't need full-time nursing care. Some assisted living facilities are part of retirement communities. Others are ...

  6. Mothers talk about their children with schizophrenia: a performance autoethnography.

    Science.gov (United States)

    Schneider, B

    2005-06-01

    This article presents the script for a performance autoethnography that explores how mothers of adults with schizophrenia talk about their children. The script draws on my own experience as a mother of a person with schizophrenia, interviews I conducted with mothers of people with schizophrenia, and written accounts by mothers of people with schizophrenia. Autoethnography refers to an approach to social science research in which the experience of the researcher is recognized as a salient part of the research process. Autoethnography typically uses non-traditional genres, such as this script, for presenting research to address ethical and political problems in the representation of research. These genres allow researchers to both break with and continue the ethnographic tradition of representing the lives and experiences of others.

  7. [Mothers and food advertising directed at children: perceptions and experiences].

    Science.gov (United States)

    Castronuovo, Luciana; Gutkowski, Patricia; Tiscornia, Victoria; Allemandi, Lorena

    2016-01-01

    The objective of this study is to analyze how food advertising is perceived by mothers from different socioeconomic sectors of the Metropolitan Area of Buenos Aires, Argentina. Between May and November 2015, eight focus groups were conducted with the participation of 49 mothers of different education levels living in the study area. The results show how the purchasing decisions of mothers are influenced by the requests of their children, which are in turn prompted by food advertising and promotion. The study also shows how food advertising and promotion are combined with other environmental factors (greater supply of food products, "more demanding" children) that affect the decision-making process of mothers regarding their children's nutrition and foster the consumption of certain unhealthy products. This situation was observed in all the focus groups, without differences among education levels.

  8. EXPERIENCES OF MOTHERS ACCOMPANYING HOSPITALIZED CHILDREN UNIT IN PEDIATRIC CLINIC

    Directory of Open Access Journals (Sweden)

    Luciano Marques dos Santos

    2014-05-01

    Full Text Available OBJECTIVE: To understand the experience of mothers accompanying hospitalized children in the Pediatric Clinic of a hospital in the interior of Bahia. METHODS: Descriptive, exploratory and qualitative study which complied with Resolution 196/96 and was developed in the Pediatric Unit, in the period between September and December 2009 through semi-structured interviews with eleven accompanying mothers. RESULTS:The illness and hospitalization occurr abruptly and generate the need for reorganization of family dynamics to meet the demands of the family members. As the accompanying mother will understand the dynamics of health workers, they stay calmer and try to understand the disease, care and her responsabilities. CONCLUSIONS: The accompanying mother needs to be recognized as a constant in the lives of sick children and within their family context, so that their actions and reactions child during hospitalization.

  9. Support for breastfeeding mothers.

    Science.gov (United States)

    Britton, C; McCormick, F M; Renfrew, M J; Wade, A; King, S E

    2007-01-24

    There is extensive evidence of the benefits of breastfeeding for infants and mothers. In 2003, the World Health Organization (WHO) recommended infants be fed exclusively on breast milk until six months of age. However, breastfeeding rates in many developed countries continue to be resistant to change. To assess the effectiveness of support for breastfeeding mothers. We searched the Cochrane Pregnancy and Childbirth Group's Trials Register (January 2006), MEDLINE (1966 to November 2005), EMBASE (1974 to November 2005) and MIDIRS (1991 to September 2005). Randomised or quasi-randomised controlled trials comparing extra support for breastfeeding mothers with usual maternity care. Two authors independently assessed trial quality and extracted data. We have included 34 trials (29,385 mother-infant pairs) from 14 countries. All forms of extra support analysed together showed an increase in duration of 'any breastfeeding' (includes partial and exclusive breastfeeding) (relative risk (RR) for stopping any breastfeeding before six months 0.91, 95% confidence interval (CI) 0.86 to 0.96). All forms of extra support together had a larger effect on duration of exclusive breastfeeding than on any breastfeeding (RR 0.81, 95% CI 0.74 to 0.89). Lay and professional support together extended duration of any breastfeeding significantly (RR before 4-6 weeks 0.65, 95% 0.51 to 0.82; RR before 2 months 0.74, 95% CI 0.66 to 0.83). Exclusive breastfeeding was significantly prolonged with use of WHO/UNICEF training (RR 0.69, 95% CI 0.52 to 0.91). Maternal satisfaction was poorly reported. Additional professional support was effective in prolonging any breastfeeding, but its effects on exclusive breastfeeding were less clear. WHO/UNICEF training courses appeared to be effective for professional training. Additional lay support was effective in prolonging exclusive breastfeeding, while its effects on duration of any breastfeeding were uncertain. Effective support offered by professionals and

  10. Psychosocial adjustment process of mothers caring for young men with traumatic brain injury: focusing on the mother-son relationship.

    Science.gov (United States)

    Fumiyo, Ishikawa; Sumie, Suzuki; Akiko, Okumiya; Yasuko, Shimizu

    2009-10-01

    This study focuses on the mother-son relationship of 13 mothers caring for single sons between the ages of 15 and 30 years who have higher brain dysfunction due to a traumatic brain injury. We aimed to elucidate the psychosocial adjustment process of mothers for 5 years following the injury to facilitate the social rehabilitation of both mother and son. Data obtained through a semistructured interview method were analyzed using a modified grounded theory approach. After 56 concepts were identified, 18 categories and then 8 core categories were semantically created. The 8 core categories were (a) avoid contact with son, (b) support son based on mother's own desire, (c) support son alone without external support, (d) realize mother's care limits and seek external support sources, (e) request and utilize external support, (f) support and work toward son's independence while monitoring his interactions with others, (g) mother considers own feelings and reenters society, and (h) strive for continued care of son with mother's acquired energy and efficient management skills. These were grouped into the following five stages: (a) avoidance, (b-c) closed, (d) support seeking, (e-f) withdrawal, and (g-h) reconstruction.

  11. The Design of Simple Bacterial Microarrays: Development towards Immobilizing Single Living Bacteria on Predefined Micro-Sized Spots on Patterned Surfaces.

    Directory of Open Access Journals (Sweden)

    Nina Bjørk Arnfinnsdottir

    Full Text Available In this paper we demonstrate a procedure for preparing bacterial arrays that is fast, easy, and applicable in a standard molecular biology laboratory. Microcontact printing is used to deposit chemicals promoting bacterial adherence in predefined positions on glass surfaces coated with polymers known for their resistance to bacterial adhesion. Highly ordered arrays of immobilized bacteria were obtained using microcontact printed islands of polydopamine (PD on glass surfaces coated with the antiadhesive polymer polyethylene glycol (PEG. On such PEG-coated glass surfaces, bacteria were attached to 97 to 100% of the PD islands, 21 to 62% of which were occupied by a single bacterium. A viability test revealed that 99% of the bacteria were alive following immobilization onto patterned surfaces. Time series imaging of bacteria on such arrays revealed that the attached bacteria both divided and expressed green fluorescent protein, both of which indicates that this method of patterning of bacteria is a suitable method for single-cell analysis.

  12. Detection of the free living amoeba Naegleria fowleri by using conventional and real-time PCR based on a single copy DNA sequence.

    Science.gov (United States)

    Régoudis, Estelle; Pélandakis, Michel

    2016-02-01

    The amoeba-flagellate Naegleria fowleri is a causative agent of primary amoebic meningoencephalitis (PAM). This thermophilic species occurs worldwide and tends to proliferate in warm aquatic environment. The PAM cases remain rare but this infection is mostly fatal. Here, we describe a single copy region which has been cloned and sequenced, and was used for both conventional and real-time PCR. Targeting a single-copy DNA sequence allows to directly quantify the N. fowleri cells. The real-time PCR results give a detection limit of 1 copy per reaction with high reproducibility without the need of a Taqman probe. This procedure is of interest as compared to other procedures which are mostly based on the detection of multi-copy DNA associated with a Taqman probe. Copyright © 2015 Elsevier Inc. All rights reserved.

  13. Assisted Living

    Science.gov (United States)

    ... it, too. Back to top What is the Cost for Assisted Living? Although assisted living costs less than nursing home ... Primarily, older persons or their families pay the cost of assisted living. Some health and long-term care insurance policies ...

  14. Working Class Mothers and School Life: Exploring the Role of Emotional Capital

    Science.gov (United States)

    Gillies, Val

    2006-01-01

    This paper explores the emotional resources generated by working class mothers to support their children at school. Analysis of material from qualitative interview research with a range working class mothers will focus on specific accounts of children's school lives to reveal how situated meanings can clash with institutional expectations. By…

  15. A Study on the Mothers of Roma Children Who Are a Risk Group

    Science.gov (United States)

    Orçan, Maide; Çiçekler, Canan Yildiz; Ari, Ramazan

    2014-01-01

    This study aims to define Roma mothers' views regarding their children's education and their tendency towards crime. The study is based on an illustrative case study method. The participants of this study are mothers of Roma children between the ages of 4 and 6 who live in Konya, Turkey--more specifically in the Yenimahalle neighborhood in the…

  16. Role of Special Olympics for Mothers of Adult Athletes with Intellectual Disability

    Science.gov (United States)

    Weiss, Jonathan A.

    2008-01-01

    The role of Special Olympics in the lives of mothers of adult athletes was examined. Forty-six mothers participated in a longitudinal study, completing a parenting stress questionnaire, a measure of their child's maladaptive behavior, and a survey of athlete involvement in Special Olympics at two time periods, 42 months apart. Results confirm that…

  17. "Mommy That's the Exit.": Empowering Homeless Mothers to Support Their Children's Daily Literacy Experiences

    Science.gov (United States)

    Di Santo, Aurelia; Timmons, Kristy; Pelletier, Janette

    2016-01-01

    The present study examined how a six-week family literacy programme contributed to supporting the efforts 12 mothers living in a residential shelter were making to foster their preschool children's literacy development. We compared pre- and post-programme interviews to explore whether the mothers applied the literacy strategies discussed in the…

  18. Child Abuse Potential among Mothers of Substance-Exposed and Nonexposed Infants and Toddlers

    Science.gov (United States)

    Hogan, Tina M. Smarsh; Myers, Barbara J.; Elswick, R. K., Jr.

    2006-01-01

    Objective: The purpose of this longitudinal study was to determine whether there were differences in child abuse potential among mothers who were nonusers, drug users who accepted treatment, and drug users who rejected offers of treatment, over the first 2 years of their children's lives. Method: Participants were mothers of 140 infants,…

  19. Black Immigrant Mothers in Palm Beach County, Florida, and Their Children's Readiness for School

    Science.gov (United States)

    Rich, Lauren; Spielberger, Julie; D'Angelo, Angela Valdovinos

    2012-01-01

    This report compares the circumstances and characteristics of Black immigrant mothers in Palm Beach County, Florida, to those of Latina immigrant and Black native-born mothers, focusing on those living in distressed areas. The study also compares the early developmental outcomes of their children. When controlling for parental and child…

  20. Results of minimally invasive surgical treatment of allograft lithiasis in live-donor renal transplant recipients: a single-center experience of 3758 renal transplantations.

    Science.gov (United States)

    Sarier, Mehmet; Duman, Ibrahim; Yuksel, Yucel; Tekin, Sabri; Demir, Meltem; Arslan, Fatih; Ergun, Osman; Kosar, Alim; Yavuz, Asuman Havva

    2018-02-26

    Allograft lithiasis is a rare urologic complication of renal transplantation (RT). Our aim is to present our experience with minimally invasive surgical treatment of allograft lithiasis in our series of live-donor renal transplant recipients. In a retrospective analysis of 3758 consecutive live-donor RTs performed in our center between November 2009 and January 2017, the results of minimally invasive surgery for the treatment of renal graft lithiasis diagnosed at follow-up were evaluated. Twenty-two (0.58%) patients underwent minimally invasive surgery for renal graft lithiasis. The mean age was 41.6 years, and duration between RT and surgical intervention was 27.3 months (range 3-67). The mean stone size was 11.6 mm (range 4-29). Stones were located in the urethra in 1, bladder in 2, ureter in 9, renal pelvis in 7 and calices in 3 patients. Surgical treatment included percutaneous nephrolithotomy in 1, cystoscopic lithotripsy in 3, flexible ureteroscopic lithotripsy in 6 and rigid ureteroscopic lithotripsy in 12 patients. No major complications were observed. One patient (4.5%) who underwent flexible ureteroscopy developed postoperative urinary tract infection. All patients were stone-free except two (9%) patients who required a second-look procedure after flexible ureteroscopic lithotripsy for residual stones. Stone recurrence was not observed in any patient during a mean follow-up duration of 30.2 months (range 8-84). Renal transplant lithiasis is uncommon and minimally invasive surgical treatment is rarely performed for its treatment. Endourological surgery may be performed safely, effectively and with a high success rate in these patients.

  1. The potential role of mother-in-law in prevention of mother-to-child transmission of HIV: a mixed methods study from the Kilimanjaro region, northern Tanzania

    Directory of Open Access Journals (Sweden)

    Leshabari Sebalda

    2011-07-01

    Full Text Available Abstract Background In the Kilimanjaro region the mother-in-law has traditionally had an important role in matters related to reproduction and childcare. The aim of this study was to explore the role of the mothers-in-law in prevention of mother-to-child transmission (PMTCT service utilization and adherence to infant feeding guidelines. Methods The study was conducted during 2007-2008 in rural and urban areas of Moshi district in the Kilimanjaro region of Tanzania. Mixed methods were used and included focus group discussions with mothers-in-law, mothers and fathers; in-depth interviews with mothers-in-law, mothers, fathers and HIV-infected mothers, and a survey of 446 mothers bringing their four-week-old infants for immunisation at five reproductive and child health clinics. Results The study demonstrated that the mother-in-law saw herself as responsible for family health issues in general and child care in particular. However she received limited trust, and couples, in particular couples living in urban areas, tended to exclude her from decisions related to childbearing and infant feeding. Mothers-in-law expected their daughters-in-law to breastfeed in a customary manner and were generally negative towards the infant feeding methods recommended for HIV-infected mothers; exclusive replacement feeding and exclusive breastfeeding. Conclusions Decreasing influence of the mother-in-law and increasing prominence of the conjugal couples in issues related to reproduction and child care, reinforce the importance of continued efforts to include male partners in the PMTCT programme. The potential for involving mothers-in-law in the infant feeding component, where she still has influence in some areas, should be further explored.

  2. Factors influencing Vitamin A supplementation among mothers of ...

    African Journals Online (AJOL)

    Background: Vitamin A supplementation is one of the best-proven and most costeffective interventions to improve vitamin A status and save children's lives. Objective: To assess factors affecting practices and utilisation of Vitamin A supplementation services among mothers with children below five years attending Mbagathi ...

  3. Health and Safety Intervention with First-Time Mothers

    Science.gov (United States)

    Culp, Anne McDonald; Culp, R. E.; Anderson, J. W.; Carter, S.

    2007-01-01

    A health education program was evaluated which used child development specialists as home visitors and served a population of first-time mothers living in rural communities. The evaluation compared health and safety outcomes between intervention and control groups. The research staff, separate from the intervention staff, collected data in the…

  4. Living Technology

    DEFF Research Database (Denmark)

    2010-01-01

    This book is aimed at anyone who is interested in learning more about living technology, whether coming from business, the government, policy centers, academia, or anywhere else. Its purpose is to help people to learn what living technology is, what it might develop into, and how it might impact...... our lives. The phrase 'living technology' was coined to refer to technology that is alive as well as technology that is useful because it shares the fundamental properties of living systems. In particular, the invention of this phrase was called for to describe the trend of our technology becoming...... increasingly life-like or literally alive. Still, the phrase has different interpretations depending on how one views what life is. This book presents nineteen perspectives on living technology. Taken together, the interviews convey the collective wisdom on living technology's power and promise, as well as its...

  5. Single-molecule tracking in live Vibrio cholerae reveals that ToxR recruits the membrane-bound virulence regulator TcpP to the toxT promoter.

    Science.gov (United States)

    Haas, Beth L; Matson, Jyl S; DiRita, Victor J; Biteen, Julie S

    2015-04-01

    Vibrio cholerae causes the human disease cholera by producing a potent toxin. The V. cholerae virulence pathway involves an unusual transcription step: the bitopic inner-membrane proteins TcpP and ToxR activate toxT transcription. As ToxT is the primary direct transcription activator in V. cholerae pathogenicity, its regulation by membrane-localized activators is key in the disease process. However, the molecular mechanisms by which membrane-localized activators engage the transcription process have yet to be uncovered in live cells. Here we report the use of super-resolution microscopy, single-molecule tracking, and gene knockouts to examine the dynamics of individual TcpP proteins in live V. cholerae cells with < 40 nm spatial resolution on a 50 ms timescale. Single-molecule trajectory analysis reveals that TcpP diffusion is heterogeneous and can be described by three populations of TcpP motion: one fast, one slow, and one immobile. By comparing TcpP diffusion in wild-type V. cholerae to that in mutant strains lacking either toxR or the toxT promoter, we determine that TcpP mobility is greater in the presence of its interaction partners than in their absence. Our findings support a mechanism in which ToxR recruits TcpP to the toxT promoter for transcription activation. © 2014 John Wiley & Sons Ltd.

  6. The Impact of Maternal Employment and Family Form on Children's Sex-Role Stereotypes and Mothers' Traditional Attitudes.

    Science.gov (United States)

    MacKinnon, Carol E.; And Others

    1984-01-01

    Examined the effects of divorce and maternal employment status on mother's and children's sex-role attitudes in 60 families. Results showed working mothers, irrespective of marital status, were more liberal than nonworking mothers. Children of single-parent families appeared to be more liberal in their sex-role orientation. (JAC)

  7. Tongue-tie and breastfeeding in newborns-mothers' perspective.

    Science.gov (United States)

    Riskin, Arieh; Mansovsky, Michal; Coler-Botzer, Tzviya; Kugelman, Amir; Shaoul, Ron; Hemo, Miri; Wolff, Leslie; Harpaz, Sarit; Olchov, Zhana; Bader, David

    2014-11-01

    This study explored clinical implications of tongue-tie (TT) on breastfeeding from the mothers' perspective and evaluated the assistance provided. This was a single-center observational study using a structured survey. All newborns with TT born in 2005-2010 were identified; two controls without TT were assigned for each. Mothers were interviewed using a uniform structured questionnaire regarding breastfeeding experience, challenges, lactation consultation, and frenotomy, if performed. One hundred eighty-three TT mothers and 314 controls were interviewed. Although the overall rates of breastfeeding problems in the first month were similar (59% vs. 52%, respectively), TT mothers reported significantly more problems with latching, prolonged breastfeeding, and infant's exhaustion during feedings, but not pain or sore nipples. Breastfeeding rates at 6 months were similar. TT mothers more frequently used pumped breastmilk to supplement breastfeeding. Significantly more TT mothers sought consultation after discharge, and a significantly greater proportion of them felt that lactation consultation helped. Eighty-seven percent of the mothers were aware of their children's TT, yet only 50% associated it with breastfeeding problems. Of the TT infants at 2 years of age or older, 11.9% were reported to have speech problems. The possibility of frenotomy was mentioned to 69% of mothers, and it was performed in 35% of cases. Procedure satisfaction was generally poor, except for when done to solve breastfeeding problems. TT infants had significantly more breastfeeding problems in the first month, but similar rates and durations of breastfeeding. Early diagnosis and lactation consultation may assist mother-infant dyads substantially. Mothers whose infants underwent frenotomies for breastfeeding more frequently found the procedure alleviated breastfeeding problems.

  8. Adolescent response to having an HIV-infected mother.

    Science.gov (United States)

    Murphy, Debra A; Roberts, Kathleen Johnston; Herbeck, Diane M

    2013-01-01

    In this study, late adolescents/early adults whose mothers were living with HIV (MLH) were interviewed in order to explore their perceptions of what it had been like for them to grow up under the shadow of their mothers' illness. Adolescents were asked to describe what the difficult aspects of growing up with an HIV-positive mother were as well as what, if any, were the more rewarding aspects. Interviews were conducted in 2009-2010 with a random sample of 40 adolescents being followed up in a longitudinal assessment study. All study participants were English or Spanish speaking. Mean age was 18.9 years (SD = 1.9); 67.5% were Latino; 27.5% African-American; and 5% other/multiracial. Results revealed that growing up with an HIV-positive mother had both challenges as well as rewards. On the challenge side, adolescents mentioned six main issues: (1) disappointment regarding mothers' missing of childhood activities and events; (2) worry about mothers' health; (3) worry about the possibility of mothers' death; (4) increased burden of adult responsibilities/caregiving; (5) feelings of secrecy/stigma associated with HIV/AIDS; and (6) need to self-monitor behavior and communication to avoid maternal stress. On the positive side, adolescents mentioned three main rewarding aspects of growing up with an HIV-positive mother: increased closeness in the mother-child relationship; fostering of positive personality traits (e.g., resilience, gratitude, open-mindedness); and "perks" accorded to HIV-affected families.

  9. Promoting Teen Mothers' Mental Health.

    Science.gov (United States)

    Freed, Patricia; SmithBattle, Lee

    2016-01-01

    In this second article in a two-part series, we call for the integration of strengths-based and trauma-informed care into services for teen mothers. Nurses working with teen mothers in health clinics, schools and home visiting programs can play a pivotal role in promoting their mental health. Many teen mothers have high levels of psychological distress and histories of adverse experiences that cannot be ignored, and cannot solely be addressed by referral to mental health services. Nurses must be prepared to assess for trauma and be open to listening to teen mothers' experiences. Principles of strengths-based and trauma-informed care are complementary and can be integrated in clinical services so that teen mothers' distress is addressed and their strengths and aspirations are supported. Potential screening tools, interviewing skills and basic strategies to alleviate teen mothers' distress are discussed.

  10. Generation of a rabbit single-chain fragment variable (scFv) antibody for specific detection of Bradyrhizobium sp. DOA9 in both free-living and bacteroid forms.

    Science.gov (United States)

    Vu, Nguyen Xuan; Pruksametanan, Natcha; Srila, Witsanu; Yuttavanichakul, Watcharin; Teamtisong, Kamonluck; Teaumroong, Neung; Boonkerd, Nantakorn; Tittabutr, Panlada; Yamabhai, Montarop

    2017-01-01

    A simple and reliable method for the detection of specific nitrogen-fixing bacteria in both free-living and bacteroid forms is essential for the development and application of biofertilizer. Traditionally, a polyclonal antibody generated from an immunized rabbit was used for detection. However, the disadvantages of using a polyclonal antibody include limited supply and cross-reactivity to related bacterial strains. This is the first report on the application of phage display technology for the generation of a rabbit recombinant monoclonal antibody for specific detection and monitoring of nitrogen-fixing bacteria in both free-living form and in plant nodules. Bradyrhizobium sp. DOA9, a broad host range soil bacteria, originally isolated from the root nodules of Aeschynomene americana in Thailand was used as a model in this study. A recombinant single-chain fragment variable (scFv) antibody library was constructed from the spleen of a rabbit immunized with DOA9. After three rounds of biopanning, one specific phage-displayed scFv antibody, designated bDOA9rb8, was identified. Specific binding of this antibody was confirmed by phage enzyme-linked immunosorbent assay (phage ELISA). The phage antibody could bind specifically to DOA9 in both free-living cells (pure culture) and bacteroids inside plant nodules. In addition to phage ELISA, specific and robust immunofluorescence staining of both free-living and bacteroid forms could also be observed by confocal-immunofluorescence imaging, without cross-reactivity with other tested bradyrhizobial strains. Moreover, specific binding of free scFv to DOA9 was also demonstrated by ELISA. This recombinant antibody can also be used for the study of the molecular mechanism of plant-microbe interactions in the future.

  11. Single Molecule Imaging Deciphers the Relation between Mobility and Signaling of a Prototypical G Protein-coupled Receptor in Living Cells.

    Science.gov (United States)

    Veya, Luc; Piguet, Joachim; Vogel, Horst

    2015-11-13

    Lateral diffusion enables efficient interactions between membrane proteins, leading to signal transmission across the plasma membrane. An open question is how the spatiotemporal distribution of cell surface receptors influences the transmembrane signaling network. Here we addressed this issue by studying the mobility of a prototypical G protein-coupled receptor, the neurokinin-1 receptor, during its different phases of cellular signaling. Attaching a single quantum dot to individual neurokinin-1 receptors enabled us to follow with high spatial and temporal resolution over long time regimes the fate of individual receptors at the plasma membrane. Single receptor trajectories revealed a very heterogeneous mobility distribution pattern with diffusion constants ranging from 0.0005 to 0.1 μm(2)/s comprising receptors freely diffusing and others confined in 100-600-nm-sized membrane domains as well as immobile receptors. A two-dimensional representation of mobility and confinement resolved two major, broadly distributed receptor populations, one showing high mobility and low lateral restriction and the other showing low mobility and high restriction. We found that about 40% of the receptors in the basal state are already confined in membrane domains and are associated with clathrin. After stimulation with an agonist, an additional 30% of receptors became further confined. Using inhibitors of clathrin-mediated endocytosis, we found that the fraction of confined receptors at the basal state depends on the quantity of membrane-associated clathrin and is correlated to a significant decrease of the canonical pathway activity of the receptors. This shows that the high plasticity of receptor mobility is of central importance for receptor homeostasis and fine regulation of receptor activity. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  12. Bound by Children: Intermittent Cohabitation and Living Together Apart

    Science.gov (United States)

    Cross-Barnet, Caitlin; Cherlin, Andrew; Burton, Linda

    2011-01-01

    In this article, we examine variations in low-income mothers' patterns of intermittent cohabitation and the voluntary and involuntary nature of these unions. Intermittent cohabitation involves couples living together and separating in repeating cycles. Using Three-City Study ethnographic data, we identified 45 low-income mothers involved in these…

  13. Treatment period and medical care costs to achieve the first live birth by assisted reproductive technology are lower in the single embryo transfer period than in the double embryo transfer period: a retrospective analysis of women younger than 40 years of age.

    Science.gov (United States)

    Kawahara, Shunsuke; Ueda, Akane; Nakahori, Takashi; Honda, Tetsuro

    2017-04-01

    It was examined whether the single embryo transfer policy makes the treatment period longer for couples to achieve their first live birth by assisted reproductive technology. This study retrospectively analyzed women who started assisted reproductive technology at younger than 40 years of age in the authors' organization. The treatment periods for couples to achieve the first live birth by assisted reproductive technology, between the women who started assisted reproductive technology from 2004 to 2009 (the double embryo transfer period group, n=250), in which the double embryo transfer was predominant, and the women who started assisted reproductive technology from 2010 to 2015 (the single embryo transfer period group, n=298), in which the single embryo transfer was predominant, were compared. The age at the start of assisted reproductive technology, pregnancy rate per embryo transfer, and rate of women who achieved a live birth by assisted reproductive technology per number of women who tried assisted reproductive technology were all significantly higher in the single embryo transfer period group. Among the women who achieved a live birth by assisted reproductive technology, the incidence of multiple births and severe ovarian hyperstimulation syndrome, the treatment period, and medical care costs needed to achieve the first live birth were all significantly lower in the single embryo transfer period group. In the single embryo transfer period group, those women who were younger than 40 years of age achieved their first live birth by assisted reproductive technology more safely, quickly, and reasonably.

  14. Prognos is of living donor liver transplantation performed during different periods in infants with biliary atresia: a single-center experience with 101 cases

    Directory of Open Access Journals (Sweden)

    HUA Xiangwei

    2014-01-01

    Full Text Available ObjectiveTo compared the prognosis between infants with biliary atresia (BA who underwent living donor liver transplantation (LDLT during 2006-2009 and 2010-2012 in the Department of Liver Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine and to evaluate the prognos is of LDLT performed during different periods. MethodsA retrospective analysis was performed on the clinical data of 101 infants with BA who underwent LDLT from October 2006 to December 2012. These patients were divided into 2006-2009 group and 2010-2012 group according to the year the procedure was performed. The two groups were compared in terms of preoperative general data, intraoperative status, and treatment outcome. The Kaplan-Meier method was used for survival analysis; measurement data were analyzed by t test, and enumeration data were analyzed by Fisher exact probability test and chi-square test. ResultsThe preoperative general data showed no significant difference between the two groups. Compared with the 2006-2009 group, the 2010-2012 group had significantly less intraoperative blood loss (t=2.05, P=0.04, a significantly shorter cold ischemia time of donor liver (t=3.25, P<0.01, and significantly lower incidence of postoperative biliary complications (χ2=4.27, P=0.04, pulmonary infection (χ2=4.47, P=0.03, and acute rejection (P=0.03. The 2010-2012 group had significantly improved survival compared with the 2006-2009 group; the 1 and 2 year cumulative survival rates for the 2010-2012 group were 884% and 88.4%, respectively, versus 84.4% and 75% for the 2006-2009 group. ConclusionThe development of surgical techniques, improvement in perioperative management, and standardized follow-up can significantly increase the success rate of surgery and survival rate and reduce postoperative complications in BA patients.

  15. Mothers' perceptions of factors influencing violence in schools.

    Science.gov (United States)

    Kandakai, T L; Price, J H; Telljohann, S K; Wilson, C A

    1999-05-01

    This study investigated mothers' perceptions of factors contributing to school violence. Of 345 mothers, 225 (65%) from urban public schools and 120 (35%) from suburban public schools, significant differences in perceptions of school violence were found on the enabling factors subscale for school location. Urban school mothers were significantly more likely than suburban mothers to attribute violence problems at their child's school to the lack of dress codes, violent messages in rap music, and poor parent/teacher communication. Significant differences in perceptions of school violence were found on the reinforcing factor subscale for school location, income, family structure, and race. Mothers of low- and middle-income, single parents, and African Americans were much more optimistic about the possibility that violence prevention programs for students, parents, and teachers would work well to stop or reduce school violence than were higher-income, married, and White mothers. These mothers also were more likely to believe it was acceptable for their child to fight at school than were their counterparts.

  16. Children's emotional and behavioral problems and their mothers' labor supply.

    Science.gov (United States)

    Richard, Patrick; Gaskin, Darrell J; Alexandre, Pierre K; Burke, Laura S; Younis, Mustafa

    2014-01-01

    It has been documented that about 20% of children and adolescents suffer from a diagnosable mental or addictive disorder in the United States. The high prevalence of children's emotional and behavioral problems (EBP) might have a negative effect on their mothers' labor market outcomes because children with EBP require additional time for treatment. However, these children may require additional financial resources, which might promote mothers' labor supply. Previous studies have only considered chronic conditions in analyzing the impact of children's health on parental work activities. Moreover, most of these studies have not accounted for endogeneity in children's health. This article estimates the effects of children's EBP on their mothers' labor supply by family structure while accounting for endogeneity in children's health. We used the 1997 and 2002 Child Development Supplements (CDS) to the Panel Study of Income Dynamics (PSID). We used probit and bivariate probit models to estimate mothers' probability of employment, and tobit and instrumental variable tobit models to estimate the effects of children's EBP on their mothers' work hours. Findings show negative effects of children's EBP on their married mothers' employment and on their single mothers' work hours. © The Author(s) 2014.

  17. An exploratory study of the ways in which mothers keep their infants occupied

    Directory of Open Access Journals (Sweden)

    A. Botha

    1992-09-01

    Full Text Available The mother child relationship can help or hinder the social, emotional and intellectual development of the infant. Research has shown that the interaction between mother and child can affect the child’s cognitive development. Research has shown that mothers from the lower socio-economic groups do not stimulate their babies optimally and that this may affect the children negatively. In this study 86 underprivileged mothers from two different cultural backgrounds were asked to describe the ways in which they kept their infants occupied during the first year of their infants’ lives. The differences between the two groups are discussed and recommendations are made.

  18. Cohabitation and children's living arrangements: New estimates from the United States.

    Science.gov (United States)

    Kennedy, Sheela; Bumpass, Larry

    2008-01-01

    This paper uses the 1995 and 2002 waves of the National Survey of Family Growth to examine recent trends in cohabitation in the United States. We find increases in both the prevalence and duration of unmarried cohabitation. Cohabitation continues to transform children's family lives, as children are increasingly born to cohabiting mothers (18% during 1997-2001) or later experience their mother's entry into a cohabiting union. Consequently, we estimate that two-fifths of all children spend some time in a cohabiting family by age 12. Because of substantial missing data in the 2002 NSFG, we are unable to produce new estimates of divorce or of children's time in single-parent families. Nonetheless, our results point to the steady growth of cohabitation and to the evolving role of cohabitation in U.S. family life.

  19. Teenage Mothers Today: What We Know and How It Matters.

    Science.gov (United States)

    Mollborn, Stefanie

    2017-03-01

    Over the past two decades, births to U.S. teenagers have fallen and no longer follow overall fertility patterns. Yet the unique challenges faced by teenage mothers and their families justify continued research. Across disciplines, newer work has furthered our understanding of teenage motherhood today. In this article, I highlight four areas of progress: processes of selection into teenage motherhood, the broader consequences of teenage childbearing beyond the socioeconomic realm, heterogeneity of effects, and the application of life course principles. Emerging societal trends such as complex family structures, a stalled recovery from the recession for families of low socioeconomic status, and a rapidly evolving political environment for reproductive health care continue to challenge the lives of teenage mothers. Given that the consequences for teenagers of becoming mothers may change, continued research is needed. Shifts in policy to favor supporting teenage mothers and addressing the causes of both teenage pregnancy and social disadvantage may help improve the lives of these mothers and their families.

  20. Teenage Mothers Today: What We Know and How It Matters

    Science.gov (United States)

    Mollborn, Stefanie

    2016-01-01

    Over the past two decades, births to U.S. teenagers have fallen and no longer follow overall fertility patterns. Yet the unique challenges faced by teenage mothers and their families justify continued research. Across disciplines, newer work has furthered our understanding of teenage motherhood today. In this article, I highlight four areas of progress: processes of selection into teenage motherhood, the broader consequences of teenage childbearing beyond the socioeconomic realm, heterogeneity of effects, and the application of life course principles. Emerging societal trends such as complex family structures, a stalled recovery from the recession for families of low socioeconomic status, and a rapidly evolving political environment for reproductive health care continue to challenge the lives of teenage mothers. Given that the consequences for teenagers of becoming mothers may change, continued research is needed. Shifts in policy to favor supporting teenage mothers and addressing the causes of both teenage pregnancy and social disadvantage may help improve the lives of these mothers and their families. PMID:28533814

  1. The effect of social support around pregnancy on postpartum depression among Canadian teen mothers and adult mothers in the maternity experiences survey.

    Science.gov (United States)

    Kim, Theresa H M; Connolly, Jennifer A; Tamim, Hala

    2014-05-07

    Postpartum depression (PPD) is a mood disorder that affects 10-20 percent of women, and can begin any time during first year after delivery lasting for months. Social support may decrease risk of depression during pregnancy for women. However, literature shows that the amount of social support received during and after pregnancy is different for teen mothers and adult mothers. This study examined the effects of social support received during and after pregnancy on PPD among Canadian women and identified if the relationship was different for teen mothers compared to adult mothers. The study was based on secondary analysis of the Maternity Experiences Survey. A total of 6,421 women with singleton live births, aged 15 years and older were analyzed. Teen mothers were identified as 15-19 years old and adult mothers were identified as 20 years and older. The main outcome of the study was PPD, which was evaluated using the Edinburg Postnatal Depression Scale. The main independent variable was social support received during pregnancy and after birth. Logistic regression was computed to assess the relationship between social support and PPD after adjusting for confounding variables and age as an interaction term. Adjusted Odds Ratios and 95% Confidence Intervals were reported. PPD was experienced by 14.0% among teen mothers and 7.2% among adult mothers (p teen mothers reported receiving more support during pregnancy and after birth than adult mothers (p teen compared to adult mothers. Both teen and adult mothers were approximately five times more likely to experience PPD if they received no support or minimal support after the birth of the baby (95% CI, 3.51-7.36). Receiving social support especially after birth is important for mothers of all ages to reduce the risk of PPD.

  2. Safeguarding nutritional status of adolescent mothers

    International Nuclear Information System (INIS)

    Calderon de la Barca, A.M.; Casanueva, E.; Bolanos, A.V.; Caire Juvera, G.; Valencia, M.E.; Roman Perez, R.

    1999-01-01

    The Baby-Friendly Hospital Initiative (BFHI) has been introduced in Mexico in order to increase breast feeding. The Mexican health care system may be contributing negatively to the nutritional status of adolescent mothers and their infants due to the reduction of baby formulas which were previously supplied as a benefit. The aim of this study is to evaluate breast-milk production, the quantity and quality of protein and changes in body composition in adolescent and adult mothers in order to design the right ways to help mother and infants fulfill their requirements or obtain support to apply to the BFHI program. We propose to measure breast-milk intake and total body water by deuterium kinetics using infrared spectroscopy. Protein concentration and composition will be measured by electrophoresis. Adolescent and adult mothers who are breast-feeding exclusively and living in two different urban regions, northwest and central Mexico, will be dosed with deuterium oxide; saliva and breast milk samples will be taken. Currently, both of the working groups are standardized. A sublimation system for separation of water and D 2 O of the saliva samples under reduced pressure was designed and it has a 100% of D 2 O recovery. The setting up of conditions for D 2 O determination using infrared spectroscopy was achieved and calibration curves of absorbance of sequentially diluted D 2 O were obtained with an r=0.9948. Total body water was measured in 12 women after 1, 3, 6, 9 and 14 days of the D 2 O dose. Zero-time values obtained by extrapolation were similar to those directly obtained after 4 h equilibrium. In addition, 50 women (under inclusion criteria) have signed consent letters to participate and part of the study is currently underway. For 1998, we intend to have 40 women and their infants with both dosing periods analyzed, as well as the analysis of breast milk composition. (author)

  3. [Psychomotor development in offspring of mothers with post partum depression].

    Science.gov (United States)

    Podestá L, Loreto; Alarcón, Ana María; Muñoz, Sergio; Legüe C, Marcela; Bustos, Luis; Barría P, Mauricio

    2013-04-01

    Postpartum depression (PPD) has adverse effects on psychomotor development of the offspring. To evaluate the relationship between PPD and psychomotor development in children aged 18 months, consulting in primary care. Cross-sectional study with 360 infants and their mothers. Children had their psychomotor evaluation at l8 months and mothers completed the Edinburgh Postnatal Depression Scale at 4 and 12 weeks postpartum. The prevalence of both PPD and psychomotor alteration was estimated. The association between PPD and psychomotor alteration, including confounding variables, was estimated through logistic multiple regression analysis. The prevalence of PPD and psychomotor alteration was 29 and 16%, respectively Mothers with PPD had twice the probability of having an offspring with psychomotor alteration (Odds ratio = 2.0, confidence intervals = 1.07-3.68). This probability was significantly higher among single mothers or those with an unstable partner. PPD has a detrimental impact on psychomotor development of children.

  4. Evaluation of the single-item self-rating adherence scale for use in routine clinical care of people living with HIV.

    Science.gov (United States)

    Feldman, B J; Fredericksen, R J; Crane, P K; Safren, S A; Mugavero, M J; Willig, James H; Simoni, J M; Wilson, I B; Saag, M S; Kitahata, M M; Crane, H M

    2013-01-01

    The self-rating scale item (SRSI) is a single-item self-report adherence measure that uses adjectives in a 5-point Likert scale, from "very poor" to "excellent," to describe medication adherence over the past 4 weeks. This study investigated the SRSI in 2,399 HIV-infected patients in routine care at two outpatient primary HIV clinics. Correlations between the SRSI and four commonly used adherence items ranged from 0.37 to 0.64. Correlations of adherence barriers, such as depression and substance use, were comparable across all adherence items. General estimating equations suggested the SRSI is as good as or better than other adherence items (p's <0.001 vs. <0.001-0.99) at predicting adherence-related clinical outcomes, such as HIV viral load and CD4(+) cell count. These results and the SRSI's low patient burden suggest its routine use could be helpful for assessing adherence in clinical care and should be more widespread, particularly where more complex instruments may be impractical.

  5. [Single coronary artery originating from the left pulmonary artery of a "truncus arteriosus communis" in a living 39 year-old-patient (author's transl)].

    Science.gov (United States)

    D'Alessandro, L C; Di Lorenzo, M

    1976-01-01

    Observation of a single coronary artery with origin from left pulmonary artery in a 39 year-old man who was brought to the operating table for correction of a persistent truncus arteriosus is discussed. The anomaly is expectional and has never been described intra vitam. The authors explain the possible formal genesis on the basis of the most accepted embriogenetic theories and they analyze the special physiopathological behaviour which allowed the long survival and the good physical state of the patient which is still only slightly reduced. In this case a precedent cerebral abscess and frequent hemophtysis indicated the surgical treatment of the truncus arteriosus which was nor performed because of the finding of the coronary anomaly not previously seen with angiography. The authors emphasize the necessity of an accurate pre-operative study of the coronary tree in all patients with persistent truncus arteriosus, to avoid the possibility that anomalies of the origin and course of the coronary arteries can make radical surgical treatment difficult or impossible.

  6. In Their Own Words: The Health and Sexuality of Immigrant Women with Infibulation Living in Switzerland

    Directory of Open Access Journals (Sweden)

    Michela Villani

    2016-11-01

    Full Text Available Female genital mutilation (FGM is a significant public health problem. It is estimated that around 14,700 women affected by FGM live in Switzerland, primarily among women with a history of migration. Our qualitative research investigated the sexual health of immigrant women living with FGM in Switzerland, describing their own perception of health, reproductive life and sexuality. We conducted semi-structured, in-depth interviews with a group of eight immigrant women of sub-Saharan origin living in Switzerland with Type III FGM (infibulation. Seven of the women were from Somalia and one was from the Ivory Coast. All of the Somali women were mothers and married (two separated, and the Ivorian woman was a single mother. The women in our study reported a low level of sexual satisfaction and reproductive health. They affirmed their desire to improve, or at least change, their condition. Although they rarely talk with their husbands about sexual subject matter, they would like to include them more and improve dialogue. Specific socio-sexual management is recommended when caring for immigrant women living with FGM in order to respond to their specific health care needs. Multidisciplinary approaches may be able to offer more comprehensive health care, including facilitated communication to improve dialogue between women and health care professionals, and eventually between women and their husbands in discussing sexual subject matter.

  7. Mother-offspring associations in northern muriquis, Brachyteles hypoxanthus.

    Science.gov (United States)

    Tolentino, Karynna; Roper, James J; Passos, Fernando C; Strier, Karen B

    2008-03-01

    Maternal care of offspring is ubiquitous among primates, but its duration varies across species due to factors such as dispersal patterns and social dynamics, which influence opportunities for and potential benefits of maternal investment in older offspring, respectively. We examined mother-offspring associations in wild northern muriquis (Brachyteles hypoxanthus), in which males are philopatric, females typically disperse before puberty, and social relationships among and between males and females are egalitarian. Associations were systematically recorded between ten mothers, each with two-six offspring in the study group, and all group members from August 2003-May 2004 at the RPPN-Feliciano Miguel Abdala in Minas Gerais, Brazil. Infants of both sexes received similarly high percentages of their mothers' association time. Mothers without infants also maintained strong associations with their youngest juvenile sons. Mothers did not spend consistently more time associating with either juvenile or adult sons than daughters. Our finding of non-preferential associations between muriqui mothers and their older male offspring suggests that extended maternal investment in offspring may be of minimal value in their egalitarian society compared with its value for species living in hierarchical societies.

  8. Intimate partner violence. Mothers' perspectives of effects on their children.

    Science.gov (United States)

    Lemmey, D; McFarlane, J; Willson, P; Malecha, A

    2001-01-01

    Intimate partner violence not only affects adults but also the children living within that "war zone." The present study expands our understanding about how children are affected when they observe violence in their own homes, as reported by their mothers. This descriptive study was conducted to describe mothers' perspectives of the impact of the violence on their children. A consecutive sample of 72 mothers attempting to file assault charges were interviewed in a private room by a registered nurse and were asked to describe the effect of witnessing intimate partner violence on their child's behavior. Each response was written verbatim by the interviewer. A majority (72%) of the mothers reported negative behaviors in their children that they believed were as a result of witnessing their mother's violent experiences. The most common negative traits were distress-indicating behaviors such as sleep disturbances, clinging, and fretful behaviors followed by problems with the abuser, problems in school, and problems with mother. Because intimate partner violence affects children, health care providers should become familiar with behaviors indicative of this problem. To promote the well being and development of children, recommendations for assessment and intervention for women experiencing intimate partner violence are discussed.

  9. The effect of Transcendental Meditation on self-efficacy, perceived stress, and quality of life in mothers in Uganda.

    Science.gov (United States)

    Goldstein, Leslee; Nidich, Sanford I; Goodman, Rachel; Goodman, David

    2018-03-01

    Vulnerable women living in poverty in Uganda, who are primarily single, illiterate mothers, face high levels of physical and psychological stress. Our study assessed the impact of the Transcendental Meditation® (TM®) technique on self-efficacy, perceived stress, and mental and physical quality of life of these women. This single-blind controlled study involved eighty-one women who were assigned to either practice of the Transcendental Meditation program (n = 42) or wait-list (delayed start) control group (n = 39). Participants learned the Transcendental Meditation program over five sessions, then practiced at home for twenty minutes twice a day, and attended twice monthly group meetings over a three-month period. The primary outcome measure was self-efficacy using the General Self-Efficacy Scale (GSES). Perceived stress using Cohen's Perceived Stress Scale, and physical and mental quality of life using subscales of the Medical Outcomes Survey (MOS, HIV version) were secondary outcome measures. Significant improvements were shown in self-efficacy (p stress (p 88%. This is the first controlled study to demonstrate the effect of TM in the daily lives of mothers living in impoverished conditions. Further questionnaires were administered to participants at 8 months and at 36 months with questions about changes they may have experienced in their daily life since starting TM. "Yes", "No" self-reported answers suggested that the women experienced improved health, improved relationships with others, and increased employment rates. These findings taken as a whole have important implications for developing self-efficacy, improving mental and physical quality of life, and reducing stress in the lives of these vulnerable women.

  10. Safety and immunogenicity of single-dose live oral cholera vaccine strain CVD 103-HgR in healthy adults age 18-45.

    Science.gov (United States)

    McCarty, James M; Lock, Michael D; Hunt, Kristin M; Simon, Jakub K; Gurwith, Marc

    2018-02-01

    The attenuated recombinant Vibrio cholerae O1 vaccine strain CVD 103-HgR, re-developed as PXVX0200, elicits a rapid serum vibriocidal antibody (SVA) response and protects against cholera diarrhea in volunteer challenge studies. We performed a phase 3, placebo controlled, double blind, multi-center study to further assess the safety, immunogenicity, and lot-to-lot consistency of PXVX0200. Adult volunteers 18-45 years of age were randomized 8:1 to receive a single dose of 1 × 10 9 CFU of PXVX0200 from three production lots or saline placebo. Immunogenicity endpoints included SVA and anti-cholera toxin (CT) antibody levels on days 1, 11, 29, 91 and 181. Safety was assessed by comparing solicited signs and symptoms on days 1-8, unsolicited adverse events through day 29 and serious adverse events through day 181. A total of 3146 participants were enrolled, including 2795 vaccine and 351 placebo recipients. The SVA seroconversion rates at day 11 were 94% and 4% in the PXVX0200 and placebo recipients, respectively (P < .0001). Cumulative SVA seroconversion occurred among 96% of vaccine recipients. PXVX0200 SVA GMTs peaked on day 11 and remained significantly higher than placebo through day 181 while the fold-rise over baseline in PXVX0200 anti-CT antibody was significantly greater than placebo at every post-vaccination time point. Most reactogenicity was mild and resolved within 1-3 days with headache and diarrhea more frequently reported in PXVX0200 recipients. There were no differences in unsolicited adverse events and no study-related serious adverse events. Immunogenicity and safety endpoints were equivalent between the three production lots. PXVX0200 is immunogenic and well tolerated across multiple production lots. Clinicaltrials.gov NCT02094586. Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.

  11. Healthy Living

    Science.gov (United States)

    ... Kids Health Kids Environment Kids Health Menu Topics Environment & Health Healthy Living Pollution Reduce, Reuse, Recycle Science – How It Works ... Scientist Coloring Science Experiments Stories Lessons Topics Expand Environment & Health Healthy Living Pollution Reduce, Reuse, Recycle Science – How It Works ...

  12. Assisted Living

    Science.gov (United States)

    ... may also be higher than in other supported-living environments. Adult Foster Care Foster care homes generally provide ... board, and some help with activities of daily living. This is provided by ... more home-like environment. Regulations for foster care vary by state, and ...

  13. [Pregnant women and mothers using alcohol, tobacco and illegal drugs].

    Science.gov (United States)

    Nechanská, B; Mravčík, V; Sopko, B; Velebil, P

    2012-10-01

    - almost 2/3 of mothers using addictive substances were unmarried or didn't live in permanent partnership and more than 82% of mothers-users have lower education (primary or secondary school without a diploma). The association between substance use and induced and spontaneous abortions was observed only in smokers. Serious complications of pregnancy were associated with all monitored addictive substances - in mothers-smokers, a probability of serious complications were about 40 %, in users of illicit drugs about 13 % and in alcohol users about 5 % higher as compared to nonusers. Substance use showed almost no association with problems during childbirth. Alcohol and illegal drugs use increased probability of complications in puerperium. Health status of foetus/newborn was negatively significantly altered mainly in those born to mothers-smokers in almost all observed characteristics. Mothers alcohol use during pregnancy was associated primarily with the overall health status of foetus immediately after delivery, congenital anomalies, stillbirth or need for treatment of newborn in the theatre. Infants of mothers using addictive substances had higher probability of consequent hospitalization after discharge from the neonatal department, transfer to infant home and death of infant. Association between complications during pregnancy, delivery and puerperium and health status of newborns and substance use of mothers during pregnancy was found mainly in cigarette smoking. Alcohol use was found significant in some (but serious) health problems of mothers and newborns. The association between illegal drugs and monitored indicators wasn't found. Following the results of this work, criteria for reporting of illegal drug use in mothers during pregnancy should be improved.

  14. Adolescent Mothers' Adjustment to Parenting.

    Science.gov (United States)

    Samuels, Valerie Jarvis; And Others

    1994-01-01

    Examined adolescent mothers' adjustment to parenting, self-esteem, social support, and perceptions of baby. Subjects (n=52) responded to questionnaires at two time periods approximately six months apart. Mothers with higher self-esteem at Time 1 had better adjustment at Time 2. Adjustment was predicted by Time 2 variables; contact with baby's…

  15. Literacy and the Mother Tongue

    Science.gov (United States)

    Literacy Work, 1974

    1974-01-01

    Reviewing the situation of literacy in the mother tongue, the article reports on projects in: (1) Africa--Mali and Nigeria, (2) the Amazonian jungle of Peru in Latin America, and (3) Papua, New Guinea. Psychological, sociological, and educational advantages of the mother tongue are discussed. (MW)

  16. How low-income mothers with overweight preschool children make sense of obesity.

    Science.gov (United States)

    Hughes, Cayce C; Sherman, Susan N; Whitaker, Robert C

    2010-04-01

    Epidemiologic and qualitative studies have found that most mothers with overweight preschool children do not think their children are overweight. This might present a challenge for clinicians who wish to address obesity in young children. To understand mothers' perceptions of their overweight children's weight, we conducted semistructured interviews with 21 mothers of overweight preschool children enrolled in Kentucky's Special Supplemental Nutrition Program for Women, Infants, and Children. Although these mothers did not label their children as overweight, they were worried about children's weight, particularly as it related to their emotional well-being. These worries about obesity were reflected in three central tensions that shaped the way mothers perceived their children's weight and informed maternal feeding strategies: (a) nature vs. nurture, (b) medical authority vs. lived experience, and (c) relieving immediate stress vs. preventing long-term consequences. Acknowledging mothers' concerns and tensions might help clinicians communicate more effectively with them about obesity.

  17. An Exploration of Jamaican Mothers' Perceptions of Closeness and Intimacy in the Mother-Child Relationship during Middle Childhood.

    Science.gov (United States)

    Burke, Taniesha; Kuczynski, Leon; Perren, Sonja

    2017-01-01

    Research on Jamaican mother-child relationships has had a limited focus on authoritarian parenting styles and selected discipline practices such as corporal punishment. This study examined Jamaican mothers' experiences of closeness and connectedness with their children to provide a holistic perspective on Jamaican-parent-child relationships. Thirty mothers (17 middle class and 13 lower class) living in Kingston and St. Andrew, Jamaica, participated in a 1-h to 1.5-h semi-structured, open-ended interview regarding their 8- to 12-year-old children. Thematic analyses indicated that mothers experienced closeness through intimate interactions (e.g., shared projects, shared physical affection, mutuality, and child self-disclosure) and parent-child nurturance. Both mothers and children were active in creating contexts for closeness. Mothers also reported experiences that temporarily damaged their connection with their children. The findings suggest that the construct of parent-child intimacy may be useful in teasing out the psychological meanings and interpersonal processes of parent-child relatedness in cultural research.

  18. Parenting stress and social support in hearing mothers of deaf and hearing children: a longitudinal study.

    Science.gov (United States)

    Lederberg, Amy R; Golbach, Traci

    2002-01-01

    This longitudinal study investigated the impact of child deafness on mothers' stress, size of social networks, and satisfaction with social support. Twenty-three hearing mothers of deaf children and 23 hearing mothers of hearing children completed a series of self-report questionnaires when their children were 22 months, 3, and 4 years old. When children were 22 months, more mothers of deaf children reported pessimism about their children's achieving self-sufficiency and concerns about their children's communication abilities than did mothers of hearing children. When their children were 3 and 4 years old, mothers of deaf and hearing children did not differ in their reports of general parenting stress, as measured by the Parenting Stress Index (PSI). Likewise, mothers' ratings of satisfaction with social support were not affected by child deafness, nor did they change developmentally. Mothers of deaf and hearing children did differ in the types of support networks utilized. Mothers of deaf 22-month-olds reported significantly larger professional support networks, while mothers of hearing children reported significantly larger general support networks across all child ages. Mothers' feelings of stress and satisfaction with social support were very stable across the 2 years examined. The results suggest that most mothers of deaf children do not feel a high level of general parenting stress or dissatisfaction with their lives and support networks. However, mothers of deaf children are likely to feel stress in areas specific to deafness. In addition, because parenting stress was highly stable, special efforts should be made to intervene when mothers of deaf children are expressing high levels of stress.

  19. Like mother, like daughter

    Indian Academy of Sciences (India)

    Lawrence

    using Casteism to justify Racism, or the other way round. I chose to be a scientist. I chose to live and work in India. I chose to have a child and a family. If the Scientific establishment chooses to discriminate against others like me, they are losing about half the brains that this country produces. This is a natural re- source India ...

  20. Coresidence and Geographic Proximity of Mothers and Adult Children in Stepfamilies

    Science.gov (United States)

    Seltzer, Judith A.; Yahirun, Jenjira J.; Bianchi, Suzanne M.

    2013-01-01

    Children who live with or near a parent provide more care and receive more help from parents than geographically distant children. Stepfamily ties may be weaker than ties between biological kin, but little is known about the geographic proximity of step- versus biological kin. The authors used data from the Health and Retirement Study (N = 13,239 mothers and 45,675 biological and stepchildren) to show that stepchildren and stepmothers are less likely to live together, less likely to live nearby, and less likely to move closer than biological children and mothers. When mothers have only stepchildren, they are less likely to have a coresident child or a child nearby than mothers with both step- and biological children. Coresidence and geographic proximity are lower in stepfamilies formed after divorce than after widowhood. The findings are consistent with a legacy of conflict and strain and the likely competing needs of biological and stepmothers. PMID:25364039

  1. Single live cell TGF-β signalling imaging: breast cancer cell motility and migration is driven by sub-populations of cells with dynamic TGF-β-Smad3 activity.

    Science.gov (United States)

    Luwor, Rodney B; Hakmana, Dulani; Iaria, Josephine; Nheu, Thao V; Simpson, Richard J; Zhu, Hong-Jian

    2015-02-22

    Metastasis is a process where only a small subset of cells is capable of successfully migrating to and propagating at secondary sites. TGF-β signalling is widely known for its role in cancer metastasis and is associated with cell migration in whole cell populations. We extend these findings by investigating the role of TGF-β signalling in promoting migration and motility by imaging the signalling activity in live, individual MDA-MB-231 cancer cells utilizing a novel Smad3 Td-Tomato reporter adenovirus. Here we find that not all MDA-MB-231 cancer cells have similar TGF-β mediated Smad3 transcription activity and display at least two distinct migratory populations. Importantly, Smad3 activity was significantly higher within migratory cells compared to non-migrated cells in wound healing and transwell assays. Furthermore, time-lapse experiments showed that MDA-MB-231 cells displaying Smad3 activity moved faster and a greater distance compared to cells not displaying Smad3 reporter activity. Interestingly, despite being more motile than cells with undetectable levels of Smad3 activity, high Smad3 activity was detrimental to cell motility compared to low and medium level of Smad3 activity. We have developed a method enabling real-time visualization of TGF-β signalling in single live cells. Breast cancer cell motility and migration is driven by sub-populations of cells with dynamic TGF-β-Smad3 activity. Those sub-populations may be responsible for tumor invasion and metastasis.

  2. Under-five mortality among mothers employed in agriculture: findings from a nationally representative sample

    Directory of Open Access Journals (Sweden)

    Rajvir Singh

    2015-01-01

    Full Text Available Background. India accounts for 24% to all under-five mortality in the world. Residence in rural area, poverty and low levels of mother’s education are known confounders of under-five mortality. Since two-thirds of India’s population lives in rural areas, mothers employed in agriculture present a particularly vulnerable population in the Indian context and it is imperative that concerns of this sizeable population are addressed in order to achieve MDG4 targets of reducing U5MR to fewer than 41 per 1,000 by 2015. This study was conducted to examine factors associated with under-five mortality among mothers employed in agriculture.Methods. Data was retrieved from National Family Household Survey-3 in India (2008. The study population is comprised of a national representative sample of single children aged 0 to 59 months and born to mothers aged 15 to 49 years employed in agriculture from all 29 states of India. Univariate and Multivariate Cox PH regression analysis was used to analyse the Hazard Rates of mortality. The predictive power of child mortality among mothers employed in agriculture was assessed by calculating the area under the receiver operating characteristic (ROC curve.Results. An increase in mothers’ ages corresponds with a decrease in child mortality. Breastfeeding reduces child mortality by 70% (HR 0.30, 0.25–0.35, p = 0.001. Standard of Living reduces child mortality by 32% with high standard of living (HR 0.68, 0.52–0.89, 0.001 in comparison to low standard of living. Prenatal care (HR 0.40, 0.34–0.48, p = 0.001 and breastfeeding health nutrition education (HR 0.45, 0.31–0.66, p = 0.001 are associated significant factors for child mortality. Birth Order five is a risk factor for mortality (HR 1.49, 1.05–2.10, p = 0.04 in comparison to Birth Order one among women engaged in agriculture while the household size (6–10 members and ≥ 11 members is significant in reducing child mortality in comparison to ≤5 members

  3. Maternal distress explains the relationship of young African American mothers' violence exposure with their preschoolers' behavior.

    Science.gov (United States)

    Mitchell, Stephanie J; Lewin, Amy; Rasmussen, Andrew; Horn, Ivor B; Joseph, Jill G

    2011-02-01

    Adolescent mothers and their children are particularly susceptible to witnessing or directly experiencing violence. Such violence exposure predicts maternal distress, parenting, and child behavior problems. The current study examined how mothers' depressive symptoms, aggression, harsh disciplinary practices, and home environment independently explain the association between mothers' violence exposure and children's externalizing and internalizing behavior, controlling for their children's violence exposure. Data were collected from 230 African American mothers living in Washington, DC who gave birth as adolescents and whose children were 3 to 5 years old. Path analysis revealed that the effect of mothers' experienced violence on children's externalizing and internalizing behavior was mediated by mothers' depressive symptoms and aggression. However, neither harsh discipline nor stimulation in the home environment acted as significant mediators, and there were no direct or indirect effects of mothers' witnessed violence on child behavior. This study builds on previous work by identifying an association between maternal violence exposure and children's behavior, independent of children's own violence exposure that is explained by mothers' increased distress but not their parenting. These findings suggest that a potential means of preventing behavior problems in minority children born to adolescent mothers is to identify mothers who have been directly exposed to violence and treat their depressive symptoms and aggressive behaviors.

  4. Parenting under the influence: the effects of opioids, alcohol and cocaine on mother-child interaction.

    Science.gov (United States)

    Slesnick, Natasha; Feng, Xin; Brakenhoff, Brittany; Brigham, Gregory S

    2014-05-01

    Nearly 20% of adults receiving treatment for a substance use disorder live with their minor children (Stanger et al., 1999) and women in drug use treatment are twice as likely as men to have children in their household (Wechsberg et al., 1998). Parental drug use impacts the family through reduced family resources such as money and food, and researchers consistently note parenting deficits among substance users (Solis, Shadur, Burns, & Hussong, 2012). Little is known about differences in parenting and mother-child interaction among mothers with different drugs of choice or among mothers of older children, between 8 and 16 years. This study reports the findings from a sample of treatment seeking opioid, alcohol and cocaine using mothers and their 8-16-year-old child. Findings from a mother-child observational task and self-reported parenting measure indicated less undermining autonomy and higher mother maternal acceptance among opioid compared to alcohol addicted mothers. African American mothers were observed to have fewer negative interactional behaviors than Whites and both African American mothers and children self-reported higher firm control and maternal acceptance. Overall, mothers appeared to struggle with effective discipline with older versus younger children. Findings offer useful information to clinicians seeking to effectively tailor their interventions to women and children who present with different drugs of abuse, race/culture and developmental stage of child. Copyright © 2014 Elsevier Ltd. All rights reserved.

  5. Support for teenage mothers: a qualitative study into the views of women about the support they received as teenage mothers.

    Science.gov (United States)

    de Jonge, A

    2001-10-01

    To gain insight into the support teenage mothers received during pregnancy, birth and their child's pre-school years and young women's perceptions of the usefulness of a support group for teenage mothers. Most qualitative studies have focused on teenage mothers around the time of the birth of their first child. For this study, women were recruited several years after the birth (median 8.5 years), so that they would have had time to reflect on the support they had received. The qualitative method of semi-structured interviews was chosen to obtain in-depth information and to allow teenage mothers' own views to be heard. Ten individual interviews and one paired interview were undertaken. Recruitment was difficult because taking part in research was not a priority for many of the women. The study confirmed the strong link between deprivation and teenage pregnancy found in other studies, and suggested that mental health problems in teenage mothers may be more difficult to detect. Teenage women need more information on mental health and on services available to them. The fear, expressed by some of the women in this study, of becoming different from other women in their social network should be considered by health workers when establishing intervention programmes. Professional bodies of health workers should lobby government to provide a minimum standard of living and sufficient child-care to combat deprivation. Former teenage mothers should be involved in the recruitment, planning and implementation stages of research and interventions. Health professionals should be aware that mental health problems in teenage mothers may be particularly difficult to detect. Key community health workers or a support group may provide information on services, mental health and education facilities available that would benefit teenage mothers. A support group may also give emotional support.

  6. Healthy living

    Science.gov (United States)

    ... the bones, heart, and lungs, tones muscles, improves vitality, relieves depression, and helps you sleep better. Talk ... has a problem with alcohol. Many people whose lives have been affected by alcohol get benefit from ...

  7. Bachelor Living

    Science.gov (United States)

    Germer, Sondra

    1974-01-01

    Male high school students in a Bachelor Living Class observed methods of child care including bottle feeding, spoon feeding, changing diapers, and method of holding. The purpose was for the students to grasp a better understanding of child development. (EK)

  8. Live Well

    Science.gov (United States)

    ... Syndicated Content Podcasts Slide Sets Act Against AIDS Live Well Language: English Español (Spanish) Recommend on Facebook Tweet Share Compartir Being aware of your overall health beyond HIV can ...

  9. Living PSA

    International Nuclear Information System (INIS)

    Evans, M.G.K.

    1997-01-01

    The aim of this presentation is to gain an understanding of the requirements for a PSA to be considered a Living PSA. The presentation is divided into the following topics: Definition; Planning/Documentation; Task Performance; Maintenance; Management. 4 figs

  10. THE BEING MOTHER: EXPECTATIONS OF PRIMIGESTAS

    Directory of Open Access Journals (Sweden)

    Ana Paula Valasques Barretto

    2009-12-01

    Full Text Available The experience of the gestation is privilege of the women, only they can and live deeply this only moment of its lives. Amongst the factors that had contributed for the construction of this work they are: the predominant look on the physical aspects during the period of pregnancy on the part of some professionals, the increasing number of pregnant young women; amongst others. This study has qualitative boarding. Objective to know the expectations concerning the maternity in primigestas registered in cadastre in the Service of Prenatal of a Basic Unit of Health of the city of Jequié/BA; to identify the performance of the family in the support to the gestation of the primigestas; beyond inquiring if the primigestas are prepared or it does not stop exerting the maternity. The procedure used for collection of the information moved for the Committee of Ethics in Research of the southwestern State University of the Bahia, as number of protocol 029/2006. The technique used for the production of data was the half structuralized interview, having as guiding instrument the form, that contend data for to the identification of the informers and specific questions the thematic one. The citizens had consisted of 15 primigestas pregnant,registered in cadastre in the Service of Prenatal, as participation criterion all would have to be living deeply its first pregnancy, abstaining themselves the carriers of history of just-been born abortion or death of. The analysis was given by means of the technique of analysis of content of Bardin, of where categories and undercategories had emerged. The results allow to reflect on what the primigestas wait while the Being mother; point the importance of the inquiry of the emotional aspects that permeate the gestation, designating with respect to the necessity of bigger studies concerning this thematic one; e also reflects concerning the familiar support and of the preparation that these women carry through to exert the

  11. Motherhood in adolescents’ lives: Implications for action

    Directory of Open Access Journals (Sweden)

    Magaly Nóblega Mayorga

    2009-06-01

    Full Text Available Based on the findings from two qualitative studies, this article analyzes motherhood’s mean­ings for those young women living in poverty. One study explored motherhood’s meanings attributed by 25 mothers from 18 to 20 years old who had a pregnancy during adolescence. The other study examined social representations about the characteristics of early mother­hood in 24 non-parents men and women teenagers, in order to understand the culture in which the young mothers live. Results suggest that motherhood represents a positive change for most young women and it structures their identity. The implications of these findings for primary and secondary prevention in this field are discussed.

  12. Chicago Mothers on Finding and Using Child Care during Nonstandard Work Hours

    Science.gov (United States)

    Stoll, Marcia; Alexander, David; Nicpon, Christine

    2015-01-01

    Few issues confound child care policy more than the fact that very large numbers of mothers work evenings, overnight, or weekend hours when fewer child care programs operate. The authors interviewed 50 single Chicago mothers with nontraditional work hours about their experiences finding and using child care. Participants' responses addressed…

  13. Lived Lives: A Pavee Perspective

    Science.gov (United States)

    Malone, Kevin M; McGuinness, Seamus G; Cleary, Eimear; Jefferies, Janis; Owens, Christabel; Kelleher, Cecily C

    2017-04-13

    Background: Suicide is a significant public health concern, which impacts on health outcomes. Few suicide research studies have been interdisciplinary. We combined a psychobiographical autopsy with a visual arts autopsy, in which families donated stories, images and objects associated with the lived life of a loved one lost to suicide. From this interdisciplinary research platform, a mediated exhibition was created ( Lived Lives ) with artist, scientist and families, co-curated by communities, facilitating dialogue, response and public action around suicide prevention. Indigenous ethnic minorities (IEMs) bear a significant increased risk for suicide. Irish Travellers are an IEM with social and cultural parallels with IEMs internationally, experiencing racism, discrimination, and poor health outcomes including elevated suicide rates (SMR 6.6). Methods: An adjusted Lived Lives exhibition, Lived Lives: A Pavee Perspective manifested in Pavee Point, the national Traveller and Roma Centre. The project was evaluated by the Travelling Community as to how it related to suicide in their community, how it has shaped their understanding of suicide and its impacts, and its relevance to other socio-cultural contexts, nationally and internationally. The project also obtained feedback from all relevant stakeholders. Evaluation was carried out by an international visual arts research advisor and an independent observer from the field of suicide research. Results: Outputs included an arts-science mediated exhibition with reference to elevated Irish Traveller suicide rates. Digital online learning materials about suicide and its aftermath among Irish Travellers were also produced. The project reached its target audience, with a high level of engagement from members of the Travelling Community. Discussion: The Lived Lives methodology navigated the societal barriers of stigma and silence to foster communication and engagement, working with cultural values, consistent with an adapted

  14. "Mothering through Islam": Narratives of Religious Identity in London

    Directory of Open Access Journals (Sweden)

    Louise Ryan

    2013-04-01

    Full Text Available This paper draws upon research with mothers of diverse Muslim backgroundsin London to explore how these women use ‘conservative’ interpretations ofIslamic beliefs and practices to underpin their parenting strategies. In particularthe paper looks at how mothers use religion as a frame to make sense of andgive meaning to their experiences and encounters in Britain. We suggest thatthe women use Islam in four key ways: (i as a framework for teaching theirchildren right and wrong, (ii as a means of protecting children from the ‘moral’dangers of British society, (iii as an authoritative voice that reinforces parentingand (iv as a means of critiquing specific aspects of both the traditional andBritish culture in which they live and daily negotiate their different cultural andreligious belonging. In attempting to instil religious values in their London-basedchildren, these mothers have to negotiate the hostility that Islam increasinglyprovokes in British society’s public arenas.

  15. Troubling Mothers: Introduction

    Directory of Open Access Journals (Sweden)

    Imogen Tyler

    2010-01-01

    Full Text Available This panel explored a range of classed figurations of the maternal. It had two primary aims: firstly, to explore the relationship between social class and 'the maternal' and secondly to foreground the relative absence of psycho-social or psychoanalytic theoretical work on what Diane Reay has called 'The Psychic Landscape of Social Class' (2005. The data explored in this panel primarily originates from 'social spheres', popular and sensationalist media, art practice and 'everyday life'. It aimed to engage with analysis of these mediums and materials, and to initiate debate about the ways in which visible class representations and invisible class relations structure not only 'maternal publics' and also our most intimate, personal and 'interior' sense of ourselves as 'maternal subjects', as well as the ways in which 'visceral aversions, recognition, abjection and the markings of taste constitute a psychic economy of social class [that] contributes powerfully to the ways we are, feel and act' (Reay, 2005, p. 911. Four speakers presented their work on mothers, and three of these talks have been developed for publication in this issue.

  16. Robust and Balanced Immune Responses to All 4 Dengue Virus Serotypes Following Administration of a Single Dose of a Live Attenuated Tetravalent Dengue Vaccine to Healthy, Flavivirus-Naive Adults.

    Science.gov (United States)

    Kirkpatrick, Beth D; Durbin, Anna P; Pierce, Kristen K; Carmolli, Marya P; Tibery, Cecilia M; Grier, Palmtama L; Hynes, Noreen; Diehl, Sean A; Elwood, Dan; Jarvis, Adrienne P; Sabundayo, Beulah P; Lyon, Caroline E; Larsson, Catherine J; Jo, Matthew; Lovchik, Janece M; Luke, Catherine J; Walsh, Mary C; Fraser, Ellen A; Subbarao, Kanta; Whitehead, Steven S

    2015-09-01

    The 4 serotypes of dengue virus, DENV-1-4, are the leading cause of arboviral disease globally. The ideal dengue vaccine would provide protection against all serotypes after a single dose. Two randomized, placebo-controlled trials were performed with 168 flavivirus-naive adults to demonstrate the safety and immunogenicity of a live attenuated tetravalent dengue vaccine (TV003), compared with those of a second tetravalent vaccine with an enhanced DENV-2 component (TV005), and to evaluate the benefit of a booster dose at 6 months. Safety data, viremia, and neutralizing antibody titers were evaluated. A single dose of TV005 elicited a tetravalent response in 90% of vaccinees by 3 months after vaccination and a trivalent response in 98%. Compared with TV003, the higher-dose DENV-2 component increased the observed frequency of immunogenicity to DENV-2 in the TV005 trial. Both the first and second doses were well tolerated. Neither vaccine viremia, rash, nor a significant antibody boost were observed following a second dose. A single subcutaneous dose of TV005 dengue vaccine is safe and induces a tetravalent antibody response at an unprecedented frequency among vaccinees. A second dose has limited benefit and appears to be unnecessary. Studies to confirm these findings and assess vaccine efficacy will now move to populations in regions where DENV transmission is endemic. NCT01072786 and NCT01436422. © The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  17. Between the Estate and the State: Struggling to Be a "Good" Mother

    Science.gov (United States)

    Vincent, Carol; Ball, Stephen J.; Braun, Annette

    2010-01-01

    The paper draws on an ESRC-funded study of 70 families in two London locations. It focuses on a set of contemporary and contradictory political discourses that work on and through the lives of working-class mothers and thereby create tensions and impossibilities within their lives. We illustrate the ways in which these women are positioned by and…

  18. Will Education in the Mother Tongue Contribute to the Increase of Digital Literacies?

    Science.gov (United States)

    Joaquim, Guerra

    2017-01-01

    We live undoubtedly in a digital era in which younger people have more technological knowledge and use technology more than older people who mostly must adapt their lives and practices, learning how, why, and when to use digital tools. In schools, this gap is visible if we compare the curricula for teaching the Portuguese mother tongue and…

  19. Using Social Justice Group Work with Young Mothers Who Experience Homelessness

    Science.gov (United States)

    Coker, Angela D.; Meyer, Dixie; Smith, Rachelle; Price, Amber

    2010-01-01

    Research indicates (e.g., Bassuk et al., 1997) that women and children make up a large percentage of the total number of individuals living without permanent shelter. This article represents a reflective analysis of our work as group facilitators who conducted a series of personal growth groups for young mothers living in a residential facility…

  20. Being a mother of preterm multiples in the context of socioeconomic disadvantage: perceived stress and psychological symptoms.

    Science.gov (United States)

    Baptista, Joana; Moutinho, Vanessa; Mateus, Vera; Guimarães, Hercília; Clemente, Fátima; Almeida, Sara; Andrade, Maria Agostinha; Dias, Clara Paz; Freitas, Alice; Martins, Carla; Soares, Isabel

    2017-11-07

    This study aimed to examine the differences between mothers of preterm multiples and mothers of preterm singletons regarding perceived stress and maternal psychological symptoms, and to explore the putative adverse amplified effect of socioeconomic disadvantage. Ninety-five mothers of 1-year-olds born preterm participated in this cross-sectional study. Data collection was carried out in two public hospitals from Northern Portugal. To assess maternal perceived daily stress and psychological symptoms, mothers completed two questionnaires. Mothers reported on socioeconomic factors, including family poverty, parent unemployment, and low education, and two groups of family socioeconomic disadvantage were created. A child medical risk index was calculated. Results indicated that mothers of preterm multiples reported higher levels of stress than mothers of preterm singletons. Moreover, and specifically regarding psychological functioning, mothers of preterm multiples reported more symptoms than mothers of preterm singletons, but only when living in a context of socioeconomic adversity. The results of the present study have important implications for practice. Mothers of preterm multiples are at higher risk to present mental health difficulties, in comparison to mothers of singletons, especially when exposed to socioeconomic adversities. The development of psychosocial intervention programs and public policies are of decisive importance in helping mothers of multiples adjust to parenthood. Copyright © 2017 Sociedade Brasileira de Pediatria. Published by Elsevier Editora Ltda. All rights reserved.

  1. Mothers in the workforce.

    Science.gov (United States)

    Glezer, H

    1988-08-01

    child care. 4) Financial reasons are the main reasons given by women for their return to the workforce. 5) Cost and availability of child care is an important issue in determining women's workforce participation after the birth of a child. 6) 60% of women do not return to the workforce after childbirth; 90% of these women intend to do so in the future. 7) For the majority of women who give birth, motherhood emerges as their most important role in life. Almost 1/2 the women agree that a woman should give up her job if it inconveniences her husband and children. 8) 2/3 of working women can establish just as warm and secure a relationship as non-working mothers, compared with 4 out of 10 non-working mothers.

  2. Single-Molecule Visualization of Living Polymerization

    Science.gov (United States)

    2014-02-18

    77 (2011). 11 D. P. Allen , M. M. Van Wingerden & R. H. Grubbs. Well-defined silica-supported olefin metathesis catalysts. Org Lett 11, 1261-1264...magnet position), we can also probe how mechanical tension affects the catalytic kinetics of the polymerization. When the magnets are positioned far...the setup include: two rectangular NdFeB magnets mounted on a rotatable base controlled by a motor via a timing belt , collimated LED illumination

  3. Feeding a child with mealtime challenges: A mother's work.

    Science.gov (United States)

    Winston, Kristin

    2015-01-01

    The literature suggests that at least 25% of all children and up to 80% of children with developmental disabilities have some type of feeding and/or eating difficulty. Currently there is limited information in the literature related to mothers who are mothering young children with difficulties in the area of feeding and eating. Specifically there is little information available regarding how mothering a young child with difficulties in feeding and eating impacts participation in the work of mothering. This paper discusses a piece of the qualitative portion of a mixed methods study. The qualitative portion of the study sought to examine the lived experience of mothers who were mothering a young child with feeding concerns. Five women between the ages of 26 and 41 years of age agreed to participate in this portion of the study. All of the women were married and were mothering a child between 12 and 36 months experiencing concerns related to feeding. Phenomenological methods were used to complete the qualitative portion of this study. Each woman participated in one interview in her own home that lasted between one and two hours. Five themes emerged from the data analysis, feeling supported, seeking support, the range of emotions/feelings, dealing with the system, and the complexity of feeding. Two of the themes are discussed in this paper in detail as they relate to maternal work. Data from this study were analyzed in relationship to maternal work. Findings highlight the difficulty of negotiating daily mealtime occupations and support the connection between mealtime occupations and maternal work as outlined by Ruddick.

  4. Mother-Child Interaction and Early Language Skills in Children Born to Mothers with Substance Abuse and Psychiatric Problems.

    Science.gov (United States)

    J Haabrekke, Kristin; Siqveland, Torill; Smith, Lars; Wentzel-Larsen, Tore; Walhovd, Kristine B; Moe, Vibeke

    2015-10-01

    This prospective, longitudinal study with data collected at four time points investigated how maternal psychiatric symptoms, substance abuse and maternal intrusiveness in interaction were related to early child language skills. Three groups of mothers were recruited during pregnancy: One from residential treatment institutions for substance abuse (n = 18), one from psychiatric outpatient treatment (n = 22) and one from well-baby clinics (n = 30). Maternal substance abuse and anti-social and borderline personality traits were assessed during pregnancy, postpartum depression at 3 months, maternal intrusiveness in interaction at 12 months, and child language skills at 2 years. Results showed that the mothers in the substance abuse group had the lowest level of education, they were younger and they were more likely to be single mothers than the mothers in the two other groups. There was a significant difference in expressive language between children born to mothers with substance abuse problems and those born to comparison mothers, however not when controlling for maternal age, education and single parenthood. No group differences in receptive language skills were detected. Results further showed that maternal intrusiveness observed in mother-child interaction at 12 months was significantly related to child expressive language at 2 years, also when controlling for socio-demographic risk factors. This suggests that in addition to addressing substance abuse and psychiatric problems, there is a need for applying treatment models promoting sensitive caregiving, in order to enhance child expressive language skills.

  5. Stigma stories: four discourses about teen mothers, welfare, and poverty.

    Science.gov (United States)

    Kelly, D M

    1996-06-01

    This study uses a pragmatic model of discourse theory to analyze more than 700 articles about adolescent mothers published in the Canadian printed media in 1980-92. The introduction notes that feminist research has challenged the view that adolescent motherhood is caused by and perpetrates poverty and that a strong social stigma is still associated with teen pregnancy. After describing the methodology and theoretical framework used in this analysis, academic research on adolescent mothers, welfare, and poverty is criticized for using teen motherhood as a conventional scapegoat which allows the structural causes of poverty to be ignored. Discourses about teenage mothers are then described as a "stigma contest." Thus, discussion centers on 1) the bureaucratic notion that the "wrong" girls are keeping their babies, 2) the conservative framework which holds that an unwed teenager who relies on welfare and refuses to give her baby up for adoption (having properly rejected abortion) serves as the epitome of a "wrong family," and 3) oppositional discourse which provides a "wrong society" framework and is articulated in the alternative media. A "stigma-is-wrong" framework is then provided by the self-interpretation of the teen mothers who hold that the right to choose is essential and that it is inappropriate to stigmatize any choice. The bureaucratic viewpoint is the most common winner in this media contest and helps to frame the public debate and public policy about teenage motherhood and, thus, profoundly influences the daily lives of young mothers and their children by perpetuating negative stereotypes.

  6. Mothers' labor market choices and child development outcomes in Chile.

    Science.gov (United States)

    Reynolds, Sarah A; Fernald, Lia C H; Behrman, Jere R

    2017-12-01

    This paper examines associations between labor market participation of Chilean mothers and the cognitive, language, and socio-economic development of their children. Using a nationally-representative sample of 3-year-old children, we test if mothers' work intensity in the two previous years is associated with child development outcomes; data were collected in 2010 when children were one year old, and again in 2012, when they were three years old. We find that children who were three years old with mothers who worked for higher fractions of their children's lives in the previous two years perform significantly better on all tests (cognitive, language, socio-emotional) than children whose mothers had worked less, while controlling for baseline test performance. These main effects did not remain significant with the inclusion of a wide range of socio-economic, demographic control variables, however. Our results were similarly null when using an IV analysis or a propensity score matching approach. We provide descriptive information on theoretical pathways by which maternal work may influence child development. Though several of these pathways (e.g. preschool, toys, maternal stress) seem to be associated with both maternal work and child development outcomes, the pathways are not sufficiently strong to generate an association between maternal work and child development. We conclude that Chilean mothers' employment in early childhood generally does not have an effect on child development.

  7. Lateralization of mother-infant interactions in wild horses.

    Science.gov (United States)

    Karenina, Karina; Giljov, Andrey; Malashichev, Yegor

    2018-03-01

    The manifestation of behavioural lateralization has been shown to be modified by environmental conditions, life experiences, and selective breeding. This study tests whether the lateralization recently found in feral domestic horse (Equus caballus) is evident in undomesticated horses. Mother-offspring interactions were investigated in Przewalski's horse (E. ferus przewalskii) living in their natural habitat in Mongolia. Lateral position preferences during mare-foal spontaneous reunions were used as a behavioural marker of visual lateralization. Preferences were separately assessed for foals' approaches to their mothers and mares' approaches to their foals. Preference to keep the mother in the visual field of the left eye was found in various types of foals' behaviour. In slow travelling, Przewalski's foals showed stronger preference for the left eye use than feral horse foals. Population-level left-eye bias was also found in mothers approaching their foals. Our results indicate right-hemispheric dominance for control of mother-offspring interactions in Przewalski's horses, similar to what has been reported for other mammals including humans. Benefits conferred by the lateralized social processing of and responding to social stimuli may explain that the left-lateralized social behaviour is a robust trait of equine behaviour, not modified by domestication or specific environmental conditions of the population. Copyright © 2018 Elsevier B.V. All rights reserved.

  8. [Factors affecting young mothers' social and family relations after pregnancy].

    Science.gov (United States)

    Maranhão, Thatiana Araújo; Gomes, Keila Rejane Oliveira; Silva, José Mário Nunes da

    2014-05-01

    This study aimed to analyze factors affecting social and family relations of young mothers in the two-year postpartum period. This was a cross-sectional study of 464 young mothers in Teresina, Piauí State, Brazil, who gave birth during the first four months of 2006 in six maternity hospitals. Data were collected from May to December 2008 after identifying the young women in the maternity hospital records. Multivariate analysis used multinomial logistic regression. Married young women (including those in common-law marriages) were 80% less likely to have negative relations with their partners. Participants 20 to 22 years of age related 2.4 times better with their mothers than those 17 to 19 years of age. Young women not attending school showed 97% higher odds of negative changes in relations with friends, and Catholics were 50% less likely to have worse relations with friends following childbirth. Measures are needed to orient individuals living with young mothers (especially their partners and mothers) concerning the importance of support in this phase of life, particularly encouraging them to stay in school.

  9. Elderly Mothers of Adult Children with Intellectual Disability: An Exploration of a Stress Process Model for Caregiving Satisfaction

    Science.gov (United States)

    Kim, Go-en; Chung, Soondool

    2016-01-01

    Background: This study examines the utility of Pearlin's caregiving stress model for understanding the caregiving satisfaction of elderly mothers of adult children with intellectual disability. Methods: Mothers living in Seoul, Kyonggi, and Incheon who were 55 years of age or older and providing care for adult children with intellectual disability…

  10. Breastfeeding in Depressed Mother-Infant Dyads.

    Science.gov (United States)

    Field, Tiffany; Hernandez-Reif, Maria; Feijo, Larissa

    2002-01-01

    Interviewed depressed and non-depressed mothers on their breastfeeding practices and perceptions of their infants' feeding behavior. Found that, compared to non-depressed mothers, depressed mothers breast fed less often, stopped breastfeeding earlier, and scored lower on a breastfeeding confidence scale. Mothers who breastfed rather than bottle…

  11. Significant interactions between maternal PAH exposure and single nucleotide polymorphisms in candidate genes on B[ a ]P-DNA adducts in a cohort of non-smoking Polish mothers and newborns.

    Science.gov (United States)

    Iyer, Shoba; Wang, Ya; Xiong, Wei; Tang, Deliang; Jedrychowski, Wieslaw; Chanock, Stephen; Wang, Shuang; Stigter, Laura; Mróz, Elzbieta; Perera, Frederica

    2016-11-01

    Polycyclic aromatic hydrocarbons (PAH) are a class of chemicals common in the environment. Certain PAH are carcinogenic, although the degree to which genetic variation influences susceptibility to carcinogenic PAH remains unclear. Also unknown is the influence of genetic variation on the procarcinogenic effect of in utero exposures to PAH. Benzo[ a ]pyrene (B[ a ]P) is a well-studied PAH that is classified as a known human carcinogen. Within our Polish cohort, we explored interactions between maternal exposure to airborne PAH during pregnancy and maternal and newborn single nucleotide polymorphisms (SNPs) in plausible B[ a ]P metabolism genes on B[ a ]P-DNA adducts in paired cord blood samples. The study subjects included non-smoking women ( n = 368) with available data on maternal PAH exposure, paired cord adducts, and genetic data who resided in Krakow, Poland. We selected eight common variants in maternal and newborn candidate genes related to B[ a ]P metabolism, detoxification, and repair for our analyses: CYP1A1 , CYP1A2 , CYP1B1 , GSTM1 , GSTT2 , NQO1 , and XRCC1 . We observed significant interactions between maternal PAH exposure and SNPs on cord B[ a ]P-DNA adducts in the following genes: maternal CYP1A1 and GSTT2 , and newborn CYP1A1 and CYP1B1 . These novel findings highlight differences in maternal and newborn genetic contributions to B[ a ]P-DNA adduct formation and have the potential to identify at-risk subpopulations who are susceptible to the carcinogenic potential of B[ a ]P. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  12. [Woman and mother in prison, a difficult reality].

    Science.gov (United States)

    Castello, Christine

    2015-01-01

    Prison is a difficult and violent environment. Women prisoners are often in situations of frailty, social and emotional misery. Mothers who have a child under the age of 18 months are able to live with them in dedicated wings. However, although prison conditions have improved with time, discussion is needed around the development of alternatives to incarceration, such as electronic tagging. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  13. Acculturation of Pacific mothers in New Zealand over time: findings from the Pacific Islands Families study.

    Science.gov (United States)

    Schluter, Philip J; Tautolo, El-Shadan; Paterson, Janis

    2011-05-12

    The epidemiological investigation of acculturation has often been hampered by inconsistent definitions and measurement, and methodological short-comings. Adopting a bi-directional model, with good theoretical and psychometric properties, this study aimed to describe the temporal, ethnic and socio-demographic influences of acculturation for a group of Pacific mothers residing in New Zealand. Pacific mothers of a cohort of Pacific infants born at a large tertiary hospital in South Auckland in 2000 were interviewed at 6-weeks, 4-years and 6-years postpartum. At each measurement wave a home interview lasting approximately 90 minutes was conducted with each mother. Adapting the General Ethnicity Questionnaire, two scales of acculturation were elicited: one measuring New Zealand cultural orientation (NZAccult) and one measuring Pacific Islands cultural orientation (PIAccult). Acculturation scores were standardised and analysed using random intercept polynomial and piecewise mixed-effects regression models, accounting for the longitudinal nature of the repeated measured data. Mothers who immigrated to New Zealand and those who lived their lives in New Zealand were investigated separately. Overall, 1276 Pacific mothers provided 3104 NZAccult and 3107 PIAccult responses over the three measurement waves. Important and significant differences were observed in both bi-directional acculturation measures between the two maternal groups studied. New Zealand cultural orientation increased, on average, linearly with years lived in New Zealand both for immigrant mothers (0.013 per year, 95% CI: 0.012, 0.014), after adjusting for maternal age, and for mothers who lived their lives in New Zealand (0.008 per year, 95% CI: 0.06, 0.010). Immigrant mothers maintained their Pacific cultural orientation for, on average, 12 years before it began to linearly decrease with each year lived in New Zealand thereafter (-0.009 per year, 95% CI: -0.010, -0.008), after adjusting for maternal age

  14. Acculturation of Pacific mothers in New Zealand over time: findings from the Pacific Islands Families study

    Directory of Open Access Journals (Sweden)

    Schluter Philip J

    2011-05-01

    Full Text Available Abstract Background The epidemiological investigation of acculturation has often been hampered by inconsistent definitions and measurement, and methodological short-comings. Adopting a bi-directional model, with good theoretical and psychometric properties, this study aimed to describe the temporal, ethnic and socio-demographic influences of acculturation for a group of Pacific mothers residing in New Zealand. Methods Pacific mothers of a cohort of Pacific infants born at a large tertiary hospital in South Auckland in 2000 were interviewed at 6-weeks, 4-years and 6-years postpartum. At each measurement wave a home interview lasting approximately 90 minutes was conducted with each mother. Adapting the General Ethnicity Questionnaire, two scales of acculturation were elicited: one measuring New Zealand cultural orientation (NZAccult and one measuring Pacific Islands cultural orientation (PIAccult. Acculturation scores were standardised and analysed using random intercept polynomial and piecewise mixed-effects regression models, accounting for the longitudinal nature of the repeated measured data. Mothers who immigrated to New Zealand and those who lived their lives in New Zealand were investigated separately. Results Overall, 1276 Pacific mothers provided 3104 NZAccult and 3107 PIAccult responses over the three measurement waves. Important and significant differences were observed in both bi-directional acculturation measures between the two maternal groups studied. New Zealand cultural orientation increased, on average, linearly with years lived in New Zealand both for immigrant mothers (0.013 per year, 95% CI: 0.012, 0.014, after adjusting for maternal age, and for mothers who lived their lives in New Zealand (0.008 per year, 95% CI: 0.06, 0.010. Immigrant mothers maintained their Pacific cultural orientation for, on average, 12 years before it began to linearly decrease with each year lived in New Zealand thereafter (-0.009 per year, 95% CI: -0

  15. Preterm delivery among people living around Portland cement plants

    International Nuclear Information System (INIS)

    Yang, C.-Y.; Chang, C.-C.; Tsai, S.-S.; Huang, H.-Y.; Ho, C.-K.; Wu, T.-N.; Sung, F.-C.

    2003-01-01

    The Portland cement industry is the main source of particulate air pollution in Kaohsiung city. Data in this study concern outdoor air pollution and the health of individuals living in communities in close proximity to Portland cement plants. The prevalence of delivery of preterm birth infants as significantly higher in mothers living within 0-2 km of a Portland cement plant than in mothers living within 2-4 km. After controlling for several possible confounders (including maternal age, season, marital status, maternal education, and infant sex), the adjusted odds ratio was 1.30 (95% I=1.09-1.54) for the delivery of preterm infants for mothers living close to he Portland cement plants, chosen at the start to be from 0 to 2 km. These data provide further support for the hypothesis that air pollution can affect he outcome of pregnancy

  16. When mothers leave their children behind.

    Science.gov (United States)

    Schen, Cathy R

    2005-01-01

    Psychiatry has studied the effect on children of separation from their mothers or primary caregivers, but has not given equal attention to the effect on mothers of separation from their children. This article examines the current literature on separation from the mother's perspective. Following a review of the literature on mothers' attachment behaviors, as evidenced by separation from their very young children due to ordinary circumstances, attention will turn to specific populations of mothers enduring separation from their children in situations of hardship: mothers with mental illness, homeless mothers, mothers in prison, and two groups of working mothers-immigrant mothers and deployed navy mothers. Separation can be experienced as temporary, bringing on anxiety, or may involve a mother's choice between her child's safety and her own wish to keep the child near her, causing a conflict in the mother's feelings. In other situations, separation may be involuntary and long-lasting, inducing symptoms of depression, despair, and grief, all of which are characteristic of loss. The particular conditions of the separation-such as choice, control, and ongoing communication between mother and child-can mitigate the impact of the separation and transform it from a total to a partial loss. Three clinical cases of mothers forced to separate from their children in extreme circumstances are examined, with recommendations for treatment.

  17. Effects of regularly consuming dietary fibre rich soluble cocoa products on bowel habits in healthy subjects: a free-living, two-stage, randomized, crossover, single-blind intervention

    Directory of Open Access Journals (Sweden)

    Sarriá Beatriz

    2012-04-01

    Full Text Available Abstract Background Dietary fibre is both preventive and therapeutic for bowel functional diseases. Soluble cocoa products are good sources of dietary fibre that may be supplemented with this dietary component. This study assessed the effects of regularly consuming two soluble cocoa products (A and B with different non-starch polysaccharides levels (NSP, 15.1 and 22.0% w/w, respectively on bowel habits using subjective intestinal function and symptom questionnaires, a daily diary and a faecal marker in healthy individuals. Methods A free-living, two-stage, randomized, crossover, single-blind intervention was carried out in 44 healthy men and women, between 18-55 y old, who had not taken dietary supplements, laxatives, or antibiotics six months before the start of the study. In the four-week-long intervention stages, separated by a three-week-wash-out stage, two servings of A and B, that provided 2.26 vs. 6.60 g/day of NSP respectively, were taken. In each stage, volunteers' diet was recorded using a 72-h food intake report. Results Regularly consuming cocoa A and B increased fibre intake, although only cocoa B significantly increased fibre intake (p Conclusions Regular consumption of the cocoa products increases dietary fibre intake to recommended levels and product B improves bowel habits. The use of both objective and subjective assessments to evaluate the effects of food on bowel habits is recommended.

  18. A single dose of any of four different live attenuated tetravalent dengue vaccines is safe and immunogenic in flavivirus-naive adults: a randomized, double-blind clinical trial.

    Science.gov (United States)

    Durbin, Anna P; Kirkpatrick, Beth D; Pierce, Kristen K; Elwood, Daniel; Larsson, Catherine J; Lindow, Janet C; Tibery, Cecilia; Sabundayo, Beulah P; Shaffer, Donna; Talaat, Kawsar R; Hynes, Noreen A; Wanionek, Kimberli; Carmolli, Marya P; Luke, Catherine J; Murphy, Brian R; Subbarao, Kanta; Whitehead, Stephen S

    2013-03-15

    Dengue virus (DENV) causes hundreds of millions of infections annually. Four dengue serotypes exist, and previous infection with one serotype increases the likelihood of severe disease with a second, heterotypic DENV infection. In a randomized, placebo-controlled study, the safety and immunogenicity of 4 different admixtures of a live attenuated tetravalent (LATV) dengue vaccine were evaluated in 113 flavivirus-naive adults. Serum neutralizing antibody levels to all 4 dengue viruses were measured on days 0, 28, 42, and 180. A single dose of each LATV admixture induced a trivalent or better neutralizing antibody response in 75%-90% of vaccinees. There was no significant difference in the incidence of adverse events between vaccinees and placebo-recipients other than rash. A trivalent or better response correlated with rash and with non-black race (P vaccine viremia. TV003 induced a trivalent or greater antibody response in 90% of flavivirus-naive vaccinees and is a promising candidate for the prevention of dengue. Race was identified as a factor influencing the infectivity of the LATV viruses, reflecting observations of the effect of race on disease severity in natural dengue infection.

  19. An automated image analysis framework for segmentation and division plane detection of single live Staphylococcus aureus cells which can operate at millisecond sampling time scales using bespoke Slimfield microscopy

    Science.gov (United States)

    Wollman, Adam J. M.; Miller, Helen; Foster, Simon; Leake, Mark C.

    2016-10-01

    Staphylococcus aureus is an important pathogen, giving rise to antimicrobial resistance in cell strains such as Methicillin Resistant S. aureus (MRSA). Here we report an image analysis framework for automated detection and image segmentation of cells in S. aureus cell clusters, and explicit identification of their cell division planes. We use a new combination of several existing analytical tools of image analysis to detect cellular and subcellular morphological features relevant to cell division from millisecond time scale sampled images of live pathogens at a detection precision of single molecules. We demonstrate this approach using a fluorescent reporter GFP fused to the protein EzrA that localises to a mid-cell plane during division and is involved in regulation of cell size and division. This image analysis framework presents a valuable platform from which to study candidate new antimicrobials which target the cell division machinery, but may also have more general application in detecting morphologically complex structures of fluorescently labelled proteins present in clusters of other types of cells.

  20. Experience of dealing with moral responsibility as a mother with cancer.

    Science.gov (United States)

    Elmberger, Eva; Bolund, Christina; Lützén, Kim

    2005-05-01

    This study explored how women with a diagnosis of cancer (lymphoma) deal with moral concerns related to their responsibility as parents. Ten women with cancer and who had children living at home were interviewed. The interviews were analysed according to the constant comparative method used in grounded theory. In order to provide a focus for the analysis, the ethics of care and the concept of mothering were used as sensitizing concepts. The core concept 'experience of dealing with moral responsibility of being a parent with cancer by redefining oneself as a mother was identified. The processes involved were: interrupted mothering; facing the life-threatening illness and children's reactions; striving to be a good mother; attempting to deal with moral responsibility; and coming to terms with being a mother.

  1. Study of knowledge rate of parturient mothers about breast feeding adventages

    Directory of Open Access Journals (Sweden)

    mahnaz mardani

    2008-02-01

    - knowledge was 31. In other words, mothers who have more children, have more knowledge. There is significant relationship between the mother’s education and her knowledge which means the higher educated the mother was the more knowledge she had. The mothers who worked outside of home had more knowledge compared to those whom didn’t. In this research, it was shown that there isn any sinificant relationship between family income and the mothers knowledge. However there has been a reasonable relationship between mothers educations and their knowledge. More education, more knowledge about the importance of breast – feeding. Among working mothers, we can see that their knowledge is more than housewives. Mothers living in cities have more knowledge more than whom live in villages. Conclusion: According to findings, the amount of knowledge is relatively good, but still, most of them have not required knowledge about children nourishing, espetially those mothers whom are young and those whom live in villages. Therefore, we must pay more attention to mothers who live in villages.

  2. Teenage Pregnancy: Impact of the Integral Attention Given to the Pregnant Teenager and Adolescent Mother as a Protective Factor for Repeat Pregnancy

    Directory of Open Access Journals (Sweden)

    Maria José Carvalho Sant'Anna

    2007-01-01

    Full Text Available The purpose of this study was to evaluate the impact of the integral attention to the health of pregnant adolescents and adolescent mothers, having follow-up from the Integral Support Program for the Pregnant Teen (ISPPT, with the intention to determine quality of life and prevent repeat pregnancy. A prospective study comprised 85 adolescents attended by the ISPPT between January 2002 and June 2006 who participated in meetings during pregnancy with a multidisciplinary team that provided orientation concerning family planning, self-esteem, pregnancy prevention, motivation to continue education and/or work, and evaluate the postpartum mother-child relationship. The following were analyzed: education level, marital status, contraceptive use, thoughts and attempts at abortion, repeat pregnancy. This study was approved by the Human Research Ethics Committee. The Epi-Info v6.0b software was used for data and result evaluation using the means and the chi-squared test. The mean age of the adolescents was 15.7 years, 3.52% had repeat pregnancy within a mean follow-up of 23 months after childbirth, the mean education level was 8.1 years, 30.5% dropped out of school, with 79.4% occurring before pregnancy, 64.6% used no contraceptives, 68.3% were single, and 81.3% had a positive role model. One year after birth, 67.5% studied, 50% worked, 55.1% lived with the partner, 77% correctly used contraceptives, every child lived with their mothers and their vaccinations were up to date. The results demonstrate that the global attention given to the health of adolescent mothers and pregnant adolescents is a protective factor for pregnancy relapse and quality of life.

  3. Teenage pregnancy: impact of the integral attention given to the pregnant teenager and adolescent mother as a protective factor for repeat pregnancy.

    Science.gov (United States)

    Sant'Anna, Maria José Carvalho; Carvalho, Kepler Alencar Mendes; Melhado, Amanda; Coates, Verônica; Omar, Hatim A

    2007-02-09

    The purpose of this study was to evaluate the impact of the integral attention to the health of pregnant adolescents and adolescent mothers, having follow-up from the Integral Support Program for the Pregnant Teen (ISPPT), with the intention to determine quality of life and prevent repeat pregnancy. A prospective study comprised 85 adolescents attended by the ISPPT between January 2002 and June 2006 who participated in meetings during pregnancy with a multidisciplinary team that provided orientation concerning family planning, self-esteem, pregnancy prevention, motivation to continue education and/or work, and evaluate the postpartum mother-child relationship. The following were analyzed: education level, marital status, contraceptive use, thoughts and attempts at abortion, repeat pregnancy. This study was approved by the Human Research Ethics Committee. The Epi-Info v6.0b software was used for data and result evaluation using the means and the chi-squared test. The mean age of the adolescents was 15.7 years, 3.52% had repeat pregnancy within a mean follow-up of 23 months after childbirth, the mean education level was 8.1 years, 30.5% dropped out of school, with 79.4% occurring before pregnancy, 64.6% used no contraceptives, 68.3% were single, and 81.3% had a positive role model. One year after birth, 67.5% studied, 50% worked, 55.1% lived with the partner, 77% correctly used contraceptives, every child lived with their mothers and their vaccinations were up to date. The results demonstrate that the global attention given to the health of adolescent mothers and pregnant adolescents is a protective factor for pregnancy relapse and quality of life.

  4. Assessment of nutritional status, cognitive development, and mother-child interaction in Central American refugee children

    OpenAIRE

    Laude Monica

    1999-01-01

    A cross-sectional study was conducted between July and December 1992 to assess the nutritional status, cognitive development, and mother-child interactions in a group of 153 Nicaraguan refugee children living in Costa Rica. Nutritional status was assessed using anthropometric indices. Cognitive development was assessed with the Bayley Scale of Mental Development. Mother-child interaction was assessed with the Nursing Child Assessment Teaching Scale and Caldwell's Home Observation and Measurem...

  5. Asian American mothers' perception of their children's weight: a comparison with other racial/ethnic groups in Los Angeles.

    Science.gov (United States)

    Nobari, Tabashir Z; Wang, May-Choo; Whaley, Shannon E

    2015-01-01

    While mother's perception of child's weight is important for the success of early childhood obesity prevention programs, few studies have examined that of Asian Americans. Our study examined their perception and compared it to that of mothers of other racial/ethnic groups. Cross-sectional study of 2,051 randomly selected mothers of children aged 2-5 years living in Los Angeles County who were enrolled in the Special Supplemental Nutrition Program for Women Infants and Children (WIC). The primary outcome was mother's perception of child's weight. We found that Asian American mothers were 2.12 (95% CI: 1.27-3.54) times as likely as Hispanic mothers to accurately perceive their children's weight, adjusting for child's age, sex and birthweight, and mother's age and education. However, this relationship disappeared after adjusting for mother's BMI. We did not find differences in perception of child's weight among non-Hispanic White, non-Hispanic Black and Hispanic mothers. It appears that Asian American mothers' increased accurate perception of child's weight status can be partially explained by their lower prevalence of obesity. Our findings suggest that early childhood obesity prevention programs should consider the weight status of mothers.

  6. Ethnic differences in mothering qualities and relations to academic achievement.

    Science.gov (United States)

    Caughy, Margaret O'Brien; Mills, Britain; Owen, Margaret Tresch; Dyer, Nazly; Oshri, Assaf

    2017-10-01

    Although qualities of mothering behavior have been consistently linked with children's academic outcomes, mothers from different ethnic groups may emphasize different dimensions with their children. The present investigation aims to evaluate and compare the dimensionality of mothering in low-income African American (n = 151) and Mexican American (n = 182) mothers during early childhood and its predictive utility for children's academic achievement. Video-recorded mother-child interactions with children at 2½ and 3½ years of age were rated using 6 mothering quality items from a widely used global rating system. A bifactor measurement model of these 6 items yielded a general sensitive support factor and a specific intrusive-insensitive factor. The bifactor model fit the data significantly better at both time points than either a single-factor or a 2-factor model. Invariance testing supported the stability of the measurement model across the 2 time points. Invariance testing by ethnicity indicated differences in factor loadings as well as mean levels of the specific factor of intrusive-insensitivity. The specific factor reflecting intrusive-insensitive mothering at age 2½ years was associated with poorer subsequent reading achievement for African American but not Mexican American children, suggesting the specific factor reflected qualitatively different parenting constructs for the 2 ethnic groups. Critical examination of what constitutes more optimal parenting yielded both similar and dissimilar characteristics and their relations across culturally different groups of families. Such knowledge should contribute to the development of more effective interventions for ethnically diverse families. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

  7. Single Parenthood Impact on Street Children in Ibadan Metropolis ...

    African Journals Online (AJOL)

    FIRST LADY

    According to local laws of different nations or regions, single parenting is a two way streets, the custodial parent could be either that mother or the father, because of the role nature have bestowed upon women, most times, mothers are given custody of the children. Single parenthood Impact on Street Children in Ibadan ...

  8. Gendered Expectations? Reconsidering Single Fathers' Child-Care Time

    Science.gov (United States)

    Hook, Jennifer L.; Chalasani, Satvika

    2008-01-01

    We take a fresh look at an important question in the sociology of gender and family: Do single fathers "mother"? We add to the theoretical debate by proposing that single fathers face competing interactional pressures, to simultaneously act like mothers and men. Using nationally representative data from the American Time Use Survey 2003-2006 (N =…

  9. Vivendo no método canguru a tríade mãe-filho-família Viviendo en el método canguru la tríade madre-hijo-familia Living the mother-child-family triad in the kangaroo method

    Directory of Open Access Journals (Sweden)

    Laise Conceição Caetano

    2005-08-01

    con el hijo en el método canguro o con la familia.This study focuses on the experience of families in the Kangaroo Mother Method (KM and aims to understand the functioning of the family dynamics and to apprehend the family transformations as a result of their experience in the method. The study used Symbolic Interactionism as a theoretical and Grounded Theory as a methodological reference framework. Data revealed the following phenomena: having an unexpected evolution and outcome of pregnancy, which is a phase in which the parents go through a pregnancy and suffer due to its premature interruption; coping with the prematurity of the child, in which the parents get into the world of prematurity and face the need to act for the child's survival; and living with the decision and the experience together with the child, which reveals the possibility of staying in hospital in the KM or not. These phenomena led to the central category: weighing the risks and benefits between staying with the child in the kangaroo method or with the family.

  10. [To be a woman in Africa. On the danger of being a mother. Mortality].

    Science.gov (United States)

    Sow, F

    1994-10-01

    Losing their life while giving birth is a risk that too many women face. More than 33% of all maternal deaths each year occur in Africa, which has less than 12% of the world's population. 30-60% of deaths among African women aged 15-44 are pregnancy-related. Africa has the highest global maternal mortality rate. Its rural areas have an even greater maternal mortality rate. Common causes of maternal death are hemorrhaging, infections, anemia, vascular-renal problems, and abortion complications. In Asia and Africa, girls receive a lower quality and quantity of food than boys. They are also taken for medical care later and when in a more serious state. The lack of attention directed to medical care in early childhood is extended to adolescence, when girls are exposed to the risks of pregnancy and premature births. The culture protects young single mothers less than young wives. Single mothers hide their pregnancy and avoid medical visits. Others try to terminate the pregnancy. In developing countries, 25% of pregnancies are terminated. Menopausal women in Africa also face health risks (e.g., uterine cancer). Few underequipped health centers, lack of personnel, and the relative high cost of medical fees contribute to high maternal mortality rates. In Burkina Faso in 1985, there was only one gynecologist, one midwife, and one maternity hospital for every 225,000, 6250, and 69,230 women of reproductive age, respectively. Access to quality care is still a luxury. The recent devaluation of the CFA franc and the total destabilization of the zaire only exacerbates the awful status of women's lives in Africa. Priorities should be: ensuring prevention and treatment of obstetrical problems, increasing information on pregnancy risks, making family planning services accessible, and improving the quality of care. The most important priority is to let women have control over their own bodies, sexuality, and fertility. An inalienable right of women is to not have to die during

  11. Anxiety levels in mothers of children with specific learning disability

    Directory of Open Access Journals (Sweden)

    Karande S

    2009-01-01

    Full Text Available Background : Parents of children with specific learning disability (SpLD undergo stress in coping with their child′s condition. Aim : To measure the levels of anxiety and find out the cause of anxiety in mothers of children with SpLD at time of diagnosis. Settings and Design : Prospective rating-scale and interview-based study conducted in our clinic. Materials and Methods : One hundred mothers of children (70 boys, 30 girls with SpLD were interviewed using the Hamilton anxiety rating scale (HAM-A and a semi-structured questionnaire. Detailed clinical and demographic data of mothers were noted. Statistical Analysis : Chi-square test or unpaired student′s t-test was applied wherever applicable. Results : The mean age of mothers was 40.14 years (±SD 4.94, range 25.07-54.0, 73% belonged to upper or upper middle socioeconomic strata of society, 67% were graduates or postgraduates, 58% were full-time home-makers, and 33% lived in joint families. Levels of anxiety were absent in 24%, mild in 75%, and moderate in 1% of mothers. Their mean total anxiety score was 5.65 (±SD 4.75, range 0-21, mean psychic anxiety score was 3.92 (±SD 3.11, range 0-13, and mean somatic anxiety score was 1.76 (±SD 2.05, range 0-10. Their common worries were related to child′s poor school performance (95%, child′s future (90%, child′s behavior (51%, and visits to our clinic (31%. Conclusion : Most mothers of children with SpLD have already developed mild anxiety levels by the time this hidden disability is diagnosed. These anxieties should be addressed by counseling to ensure optimum rehabilitation of these children.

  12. Lively package

    International Nuclear Information System (INIS)

    Jaremko, G.

    1997-01-01

    Progress on the Lloydminster Heavy Oil Interpretive Centre, sponsored by the Lloydminster Oilfield Technical Society and expected to open in late 1998, was discussed. Some $150,000 of the $750,000 budget is already in the bank, and another $150,000 is in the pipeline. The Centre will be added to an existing and well-established visitor's site. It is reported to contain a lively and imaginatively-designed exhibit package, and promises to become a combination of educational tool and tourist attraction for the town of Lloydminster, Saskatchewan, in the heart of heavy oil country

  13. Factors Which Affect the Depression of Mothers with Cerebral Palsy Child

    Directory of Open Access Journals (Sweden)

    Zahra Ahmadizadeh

    2014-12-01

    Full Text Available Objectives: The high number of cerebral palsy, the severe disability of this disorder, depression in disabilities, and long lasting effects of the child`s disability on the family especially mothers are the common issues. The purpose of this study was investigating the level of depression in mothers with cerebral palsy children and its related factors. Methods: This caused comparative study was carried out in Tehran, in 2011. Two groups of mothers with 4 to 12 years old healthy and cerebral palsy children were randomly selected to participate in this study. Beck questionnaire was used to evaluate mothers’ depression level. The relationship between variables was investigated by independent T-test and Pearson’s correlation. Results: Sixty mothers with cerebral palsy children and sixty mothers with normal children as control group were participated in the study. Mean and standard deviation of age were 33.79±6.02 in mothers and 7.11±2.71 in children. Depression of mothers with cerebral palsy child was significantly higher than control group and there was a significant correlation between depression of mothers with cerebral palsy children, and increasing caring time, dependency in activity of daily living and children`s gross and fine movements ability. Discussion: Although depression was higher in mothers with cerebral palsy children, the depression level of these mothers was affected by some factors related to the child issue. In order to decrease undesirable effects of having a cerebral palsy child, it is necessary to emphasize on children`s abilities to achieve maximum evolution potential and provide physical and mental protections for their mothers.

  14. Analysing the Experience of Motherhood Among Adolescents Living With HIV

    OpenAIRE

    Gabriela Cássia Ritt; Marco Daniel Pereira; Daniela Centenaro Levandowski

    2017-01-01

    AimAdolescent motherhood is considered a condition of vulnerability that can be further complicated by the presence of HIV infection, but little is known about how adolescent mothers experience this process. The aim of this study was to analyse the experience of motherhood among adolescents living with HIV.MethodSeven mothers (15-21 years) recruited in specialized services in Porto Alegre/Brazil, whose babies’ ages ranged from four to six months, were interviewed. Interviews were tape-recorde...

  15. Bound by Children: Intermittent Cohabitation and Living Together Apart

    OpenAIRE

    Cross-Barnet, Caitlin; Cherlin, Andrew; Burton, Linda

    2011-01-01

    In this article, we examine variations in low-income mothers' patterns of intermittent cohabitation and the voluntary and involuntary nature of these unions. Intermittent cohabitation involves couples living together and separating in repeating cycles. Using Three-City Study ethnographic data, we identified 45 low-income mothers involved in these arrangements, 18 of whom resided with their children's fathers occasionally while saying that they were not in a cohabiting relationship. We term su...

  16. Child adoption: The psycho trauma of an adolescent single mother ...

    African Journals Online (AJOL)

    Journal of Health and Visual Sciences. Journal Home · ABOUT · Advanced Search · Current Issue · Archives · Journal Home > Vol 4, No 3 (2002) >. Log in or Register to get access to full text downloads.

  17. Food Insecurity and Depression among Single Mothers in Kolokuma ...

    African Journals Online (AJOL)

    Prof

    economic pressure and that both are positively related to parent emotional and behavioural problems (depression). These parental problems are related to harsh and inconsistent parenting and parental conflicts, and this affects the child's emotional and other behavioural problems (Conger & Conger, 2002). Thus, economic ...

  18. "My mother would worry every single time I went out..."

    DEFF Research Database (Denmark)

    Pedersen, Bodil Maria

    2008-01-01

    Personal accounts of young ethnic majority and minority women indicate that their perspectives and the personal meanings of sexualised coercion they have experienced are developed through their participation in and across diverse ethnically informed communities of practice.  A simplifying...... categorisation into ethnic groups is in danger of over-emphasising differences, as well as of overlooking common connections to underlying social/societal meanings of sexualised coercion....

  19. Depressed mothers' infants are less responsive to faces and voices.

    Science.gov (United States)

    Field, Tiffany; Diego, Miguel; Hernandez-Reif, Maria

    2009-06-01

    A review of our recent research suggests that infants of depressed mothers appeared to be less responsive to faces and voices as early as the neonatal period. At that time they have shown less orienting to the live face/voice stimulus of the Brazelton scale examiner and to their own and other infants' cry sounds. This lesser responsiveness has been attributed to higher arousal, less attentiveness and less "empathy." Their delayed heart rate decelerations to instrumental and vocal music sounds have also been ascribed to their delayed attention and/or slower processing. Later at 3-6 months they showed less negative responding to their mothers' non-contingent and still-face behavior, suggesting that they were more accustomed to this behavior in their mothers. The less responsive behavior of the depressed mothers was further compounded by their comorbid mood states of anger and anxiety and their difficult interaction styles including withdrawn or intrusive interaction styles and their later authoritarian parenting style. Pregnancy massage was effectively used to reduce prenatal depression and facilitate more optimal neonatal behavior. Interaction coaching was used during the postnatal period to help these dyads with their interactions and ultimately facilitate the infants' development.

  20. Mothering on Facebook: Exploring the Privacy/Openness Paradox

    Directory of Open Access Journals (Sweden)

    Charlotte Chalklen

    2017-05-01

    Full Text Available To post or not to post photographs of children? Is it worth commenting on potentially controversial topics such as vaccinations and breastfeeding in a public forum or even a private group? Facebook offers mothers of young children a range of affordances that were unimaginable in a pre–social networking site (SNS era, but at the same time presents a new set of dilemmas surrounding parenting in the digital domain. The SNS activity of parents brings the lives of children into online spaces in a way that builds community and social capital, but at the same time creates ethical tensions and raises a series of contemporary quandaries. Drawing upon data from a mixed-methods study, including an online survey of 117 mothers and semi-structured interviews with 17, this article examines Australian mothers’ complex concerns around Facebook and the often-uneasy balance between the need for privacy and the benefits of openness, especially in relation to sharing information about children. The findings show that while issues around privacy were one of the most commonly cited downsides to Facebook use, mothers are becoming increasingly adept at negotiating ways to protect their privacy while enjoying the benefits of openness. These negotiation tactics demonstrate a strong awareness in mothers of young children of the social–political implications of parenting in the digital domain.