Genome assortment, not serogroup, defines Vibrio cholerae pandemic strains

Energy Technology Data Exchange (ETDEWEB)

Brettin, Thomas S [Los Alamos National Laboratory; Bruce, David C [Los Alamos National Laboratory; Challacombe, Jean F [Los Alamos National Laboratory; Detter, John C [Los Alamos National Laboratory; Han, Cliff S [Los Alamos National Laboratory; Munik, A C [Los Alamos National Laboratory; Chertkov, Olga [Los Alamos National Laboratory; Meincke, Linda [Los Alamos National Laboratory; Saunders, Elizabeth [Los Alamos National Laboratory; Choi, Seon Y [SEOUL NATL. UNIV.; Haley, Bradd J [U. MARYLAND; Taviani, Elisa [U. MARYLAND; Jeon, Yoon - Seong [INTL. VACCINE INST. SEOUL; Kim, Dong Wook [INTL. VACCINE INST. SEOUL; Lee, Jae - Hak [SEOUL NATL. UNIV.; Walters, Ronald A [PNNL; Hug, Anwar [NATL. INST. CHOLERIC ENTERIC DIS.; Colwell, Rita R [U. MARYLAND

2009-01-01

Vibrio cholerae, the causative agent of cholera, is a bacterium autochthonous to the aquatic environment, and a serious public health threat. V. cholerae serogroup O1 is responsible for the previous two cholera pandemics, in which classical and El Tor biotypes were dominant in the 6th and the current 7th pandemics, respectively. Cholera researchers continually face newly emerging and re-emerging pathogenic clones carrying combinations of new serogroups as well as of phenotypic and genotypic properties. These genotype and phenotype changes have hampered control of the disease. Here we compare the complete genome sequences of 23 strains of V. cholerae isolated from a variety of sources and geographical locations over the past 98 years in an effort to elucidate the evolutionary mechanisms governing genetic diversity and genesis of new pathogenic clones. The genome-based phylogeny revealed 12 distinct V. cholerae phyletic lineages, of which one, designated the V. cholerae core genome (CG), comprises both O1 classical and EI Tor biotypes. All 7th pandemic clones share nearly identical gene content, i.e., the same genome backbone. The transition from 6th to 7th pandemic strains is defined here as a 'shift' between pathogenic clones belonging to the same O1 serogroup, but from significantly different phyletic lineages within the CG clade. In contrast, transition among clones during the present 7th pandemic period can be characterized as a 'drift' between clones, differentiated mainly by varying composition of laterally transferred genomic islands, resulting in emergence of variants, exemplified by V.cholerae serogroup O139 and V.cholerae O1 El Tor hybrid clones that produce cholera toxin of classical biotype. Based on the comprehensive comparative genomics presented in this study it is concluded that V. cholerae undergoes extensive genetic recombination via lateral gene transfer, and, therefore, genome assortment, not serogroup, should be used to

Neisseria meningitidis serogroup A capsular polysaccharide acetyltransferase, methods and compositions

Energy Technology Data Exchange (ETDEWEB)

Stephens, David S [Stone Mountain, GA; Gudlavalleti, Seshu K [Kensington, MD; Tzeng, Yih-Ling [Atlanta, GA; Datta, Anup K [San Diego, CA; Carlson, Russell W [Athens, GA

2011-02-08

Provided are methods for recombinant production of an O-acetyltransferase and methods for acetylating capsular polysaccharides, especially those of a Serogroup A Neisseria meningitidis using the recombinant O-acetyltransferase, and immunogenic compositions comprising the acetylated capsular polysaccharide.

46 CFR 30.10-45 - Ocean-TB/O.

Science.gov (United States)

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Ocean-TB/O. 30.10-45 Section 30.10-45 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS GENERAL PROVISIONS Definitions § 30.10-45 Ocean—TB/O. Under this designation shall be included all tank vessels normally navigating the waters of any ocean or...

A tool based on Ligation Detection Reaction-Universal Array (LDR-UA) for the characterization of VTEC by identification of virulence-associated and serogroup-specific genes.

Science.gov (United States)

Lauri, Andrea; Castiglioni, Bianca; Morabito, Stefano; Tozzoli, Rosangela; Consolandi, Clarissa; Mariani, Paola

2011-02-01

Verocytoxigenic Escherichia coli (VTEC) are zoonotic pathogens whose natural reservoir is represented by ruminants, particularly cattle. Infections are mainly acquired by consumption of undercooked contaminated food of animal origin, contact with infected animals and contaminated environment. VTEC O157 is the most frequently isolated serogroup from cases of human disease, however, other VTEC serogroups, such as O26, O111, O145 and O103, are increasingly reported as causing Hemolytic Uremic Syndrome (HUS) worldwide. The identification of VTEC is troublesome, hindering the development of effective prevention strategies. In fact, VTEC are morphologically indistinguishable from harmless E. coli and their pathogenic potential is not strictly dependent on the serogroup, but relies on the presence of a collection of virulence genes. We developed a diagnostic tool for VTEC based on the Ligation Detection Reaction coupled to Universal Array (LDR-UA) for the simultaneous identification of virulence factors and serogroup-associated genes. The method includes the investigation of 40 sites located in 13 fragments from 12 genes (sodCF1/F2, adfO, terB, ehxA, eae, vtx1, vtx2, ihp1, wzx, wbdI, rfbE, dnaK) and was evaluated by performing a trial on a collection of 67 E. coli strains, both VTEC and VT-negative E. coli, as well as on 25 isolates belonging to other related species. Results of this study showed that the LDR-UA technique was specific in identifying the target microorganism. Moreover, due to its higher throughput, the LDR-UA can be a valid and cheaper alternative to real time PCR-based (rt-PCR) methods for VTEC identification. Copyright © 2010 Elsevier Ltd. All rights reserved.

Evaluation of a multiplex real-time PCR method for detecting Shiga toxin-producing Escherichia coli in beef and comparison to the FSIS microbiology laboratory guidebook method

Science.gov (United States)

The “top-six” non-O157 STEC (O26, O45, O103, O111, O121, and O145) most frequently associated with outbreaks and cases of food-borne illnesses have been declared as adulterants in beef by the USDA Food Safety and Inspection Service (FSIS), and regulatory testing for these serogroups in beef began in...

The prevalence of O serogroups of Escherichia coli strains causing acute urinary tract infection in children in Iran

Directory of Open Access Journals (Sweden)

Fatemeh Emamghorashi

2011-01-01

Full Text Available The aim of present study was to determine the prevalence of O serogroups of Escherichia coli (E. coli strains that cause community-acquired urinary tract infections (UTI in children. In this study, 96 children with UTI referred to two Jahrom University-affiliated Hospitals in Iran were enrolled, during the period from August 2005 to August 2006. Drug sensitivity was tested by disk diffusion method and serotyping done by slide agglutination method. A total of 96 E. coli strains were isolated from urine samples of the study children whose age ranged from one month to 14 years. Cystitis was diagnosed in 49.2% and pyelonephritis in 50.8% of the study patients. Maximum drug resistance was seen with ampicilin (80.2% and the least with imipenem (1.1%. The most common type of O antigen was O1 (12.2%. There was significant correlation between the presence of O antigens and sensitivity to nalidixic acid and gentamicin (P < 0.05. This is the first report of E. coli serotyping in children with UTI from the south of Iran and their relation to antibiotic resistance and clinical presentation. Further studies from other parts of Iran and on other serotypes are recommended.

2,6-Diaminopyridinium bis(4-hydroxypyridine-2,6-dicarboxylato-κ3O2,N,O6ferrate(III dihydrate

Directory of Open Access Journals (Sweden)

Andya Nemati

2008-10-01

Full Text Available The reaction of iron(II sulfate heptahydrate with the proton-transfer compound (pydaH(hypydcH (pyda = pyridine-2,6-diamine; hypydcH2 = 4-hydroxypyridine-2,6-dicarboxylic acid in an aqueous solution led to the formation of the title compound, (C5H8N3[Fe(C7H3NO52]·2H2O. The anion is a six-coordinated complex with a distorted octahedral geometry around the FeIII atom. Extensive intermolecular O—H...O, N—H...O and C—H...O hydrogen bonds, involving the complex anion, (pydaH+ counter-ion and two uncoordinated water molecules, and π–π [centroid-to-centroid distance 3.323 (11 Å] and C—O...π [O–centroid distance 3.150 (15 Å] interactions connect the various components into a supramolecular structure.

Fatal case of hemolytic-uremic syndrome in an adult due to a rare serogroup O91 Entero hemorrhagic Escherichia coli associated with a Clostridium difficile infection. More than meets the eye

Directory of Open Access Journals (Sweden)

Thomas Guillard

2015-08-01

Full Text Available Hemolytic-uremic syndrome due to enterohemorrhagic Escherichia coli, belonging to serogroup O91 has rarely been described. We report here a case of post-diarrheal HUS due to EHEC O91 in an elderly patient for whom diagnosis was delayed given a previously diagnosed C. difficile infection. This case highlights the usefulness of Shiga-toxin detection.

Inactivation of shiga toxin-producing Escherichia coli in lean ground beef by gamma irradiation

Science.gov (United States)

Non-O157 serovars of Shiga Toxin-producing Escherichia coli (STEC) are now responsible for over 60% of STEC induced illnesses. The majority of illnesses caused by non-O157:H7 STEC have been due to serogroups O26, O121, O103, O45, O111, and O145, “the big/top six”, which are now considered adulterant...

Biochemical characteristics, serogroups, and virulence factors of aeromonas species isolated from cases of diarrhoea and domestic water samples in Chennai

Directory of Open Access Journals (Sweden)

Alavandi S

2003-01-01

Full Text Available PURPOSE: The objective of the present study was to delineate the differences between the clinical and environmental Aeromonas species with respect to their biochemical characteristics, serogrouping and virulence factors, in order to find a phenotypic marker of enteropathogenicity. METHODS: A total of 55 Aeromonas spp. inclusive of 19 isolates from cases of diarrhoea, and 36 from water samples comprising, 10 isolates of A. hydrophila, 21 isolates each of A. sobria, and A. caviae, two isolates of A. jandaei and one isolate of A. veronii were subjected to analysis of their biochemical characteristics, serogrouping, and virulence factors. RESULTS: Among the differences recorded in the biochemical characteristics in the three major species, the most striking characteristic was fermentation of lactose, which was observed in all the 11 A. caviae isolates recovered from water samples. None of the 10 clinical isolates of A. caviae tested fermented lactose. The clinical Aeromonas isolates belonged to seven typable serogroups, O:13, O:14, O:16, O:21, O:27, O:32 and O:35. The environmental isolates belonged to eight different serogroups, such as, O:3, O:11, O:14, O:16, O:18, O:28, O:64 and O:78 and were predominated by serotypes O:18 and O:64. Among the virulence factors tested, 89% of the environmental isolates produced b haemolysin, while only 62.3% of clinical isolates were able to do so. There was no significant difference between the clinical and environmental aeromonads with respect to their enterotoxigenicity in suckling mice in vivo, cytotoxicity in vitro in Vero cell monolayers, and ability to produce siderophores. CONCLUSION: Efforts to delineate the differences between the clinical and environmental Aeromonas spp. did not reveal significant difference between them. However, difference was observed with respect to their ability to produce b haemolysin, wherein, higher percentage of environmental isolates was haemolytic. The results also suggest

Immunochemical characterization of the O antigens of two Proteus strains, O8-related antigen of Proteus mirabilis 12 B-r and O2-related antigen of Proteus genomospecies 5/6 12 B-k, infecting a hospitalized patient in Poland.

Science.gov (United States)

Drzewiecka, Dominika; Shashkov, Alexander S; Arbatsky, Nikolay P; Knirel, Yuriy A

2016-05-01

A hospitalized 73-year-old woman was infected with a Proteus mirabilis strain, 12 B-r, isolated from the place of injection of a blood catheter. Another strain, 12 B-k, recognized as Proteus genomospecies 5 or 6, was isolated from the patient's faeces, which was an example of a nosocomial infection rather than an auto-infection. Serological investigation using ELISA and Western blotting showed that strain 12 B-k from faeces belonged to the Proteus O2 serogroup. Strain 12 B-r from the wound displayed cross-reactions with several Proteus O serogroups due to common epitopes on the core or O-specific parts of the lipopolysaccharide. Studies of the isolated 12 B-r O-specific polysaccharide by NMR spectroscopy revealed its close structural similarity to that of Proteus O8. The only difference in 12 B-r was the presence of an additional GlcNAc-linked phosphoethanolamine residue, which creates a putative epitope responsible for the cross-reactivity with Pt. mirabilis O16. The new O-antigen form could appear as a result of adaptation of the bacterium to a changing environment. On the basis of the data obtained, we suggest division of the O8 serogroup into two subgroups: O8a for strains of various Proteus species that have been previously classified into the O8 serogroup, and O8a,b for Pt. mirabilis 12 B-r, where 'a' is a common epitope and 'b' is a phosphoethanolamine-associated epitope. These findings further confirm serological and structural heterogeneity of O antigens of Proteus strains isolated lately from patients in Poland.

The evolutionary divergence of Shiga toxin-producing Escherichia coli is reflected in clustered regularly interspaced short palindromic repeat (CRISPR) spacer composition.

Science.gov (United States)

Yin, Shuang; Jensen, Mark A; Bai, Jiawei; Debroy, Chitrita; Barrangou, Rodolphe; Dudley, Edward G

2013-09-01

The Shiga toxin-producing Escherichia coli (STEC) strains, including those of O157:H7 and the "big six" serogroups (i.e., serogroups O26, O45, O103, O111, O121, and O145), are a group of pathogens designated food adulterants in the United States. The relatively conserved nature of clustered regularly interspaced short palindromic repeats (CRISPRs) in phylogenetically related E. coli strains makes them potential subtyping markers for STEC detection, and a quantitative PCR (qPCR)-based assay was previously developed for O26:H11, O45:H2, O103:H2, O111:H8, O121:H19, O145:H28, and O157:H7 isolates. To better evaluate the sensitivity and specificity of this qPCR method, the CRISPR loci of 252 O157 and big-six STEC isolates were sequenced and analyzed along with 563 CRISPR1 and 624 CRISPR2 sequences available in GenBank. General conservation of spacer content and order was observed within each O157 and big-six serogroup, validating the qPCR method. Meanwhile, it was found that spacer deletion, the presence of an insertion sequence, and distinct alleles within a serogroup are sources of false-negative reactions. Conservation of CRISPR arrays among isolates expressing the same flagellar antigen, specifically, H7, H2, and H11, suggested that these isolates share an ancestor and provided an explanation for the false positives previously observed in the qPCR results. An analysis of spacer distribution across E. coli strains provided limited evidence for temporal spacer acquisition. Conversely, comparison of CRISPR sequences between strains along the stepwise evolution of O157:H7 from its O55:H7 ancestor revealed that, over this ∼7,000-year span, spacer deletion was the primary force generating CRISPR diversity.

Pathogenic Potential to Humans of Bovine Escherichia coli O26, Scotland

Science.gov (United States)

Rosser, Tracy; Allison, Lesley J.; Courcier, Emily; Evans, Judith; McKendrick, Iain J.; Pearce, Michael C.; Handel, Ian; Caprioli, Alfredo; Karch, Helge; Hanson, Mary F.; Pollock, Kevin G.J.; Locking, Mary E.; Woolhouse, Mark E.J.; Matthews, Louise; Low, J. Chris; Gally, David L.

2012-01-01

Escherichia coli O26 and O157 have similar overall prevalences in cattle in Scotland, but in humans, Shiga toxin–producing E. coli O26 infections are fewer and clinically less severe than E. coli O157 infections. To investigate this discrepancy, we genotyped E. coli O26 isolates from cattle and humans in Scotland and continental Europe. The genetic background of some strains from Scotland was closely related to that of strains causing severe infections in Europe. Nonmetric multidimensional scaling found an association between hemolytic uremic syndrome (HUS) and multilocus sequence type 21 strains and confirmed the role of stx2 in severe human disease. Although the prevalences of E. coli O26 and O157 on cattle farms in Scotland are equivalent, prevalence of more virulent strains is low, reducing human infection risk. However, new data on E. coli O26–associated HUS in humans highlight the need for surveillance of non-O157 enterohemorrhagic E. coli and for understanding stx2 phage acquisition. PMID:22377426

Prevalence of Vibrio cholerae O1 serogroup in Assam, India: A hospital-based study.

Science.gov (United States)

Sharma, Ajanta; Dutta, Bornali Sarmah; Rasul, Elmy Samsun; Barkataki, Dipa; Saikia, Anjanamoyee; Hazarika, Naba Kumar

2017-09-01

Although cholera remains to be an important public health problem, studies on reliable population-based estimates of laboratory confirmed cholera in endemic areas are limited worldwide. The aim of this hospital-based study was to evaluate the prevalence of Vibrio cholerae serogroup in Assam, India, during 2003-2013. Stool samples/rectal swabs were collected from acute watery diarrhoea (AWD) cases during 2003-2013 and processed by standard microbiological procedures. Antibiotic sensitivity test was done following the Clinical and Laboratory Standards Institute guidelines. Year-wise epidemiological trend of cholera was analyzed. Cholera contributed to 3.93 per cent of AWD cases. In Assam, cholera was found to be more prevalent in the rural areas (6.7%) followed by the tea gardens (5.06%), urban slum (1.9%) and urban areas (1.4%). Highest proportion of cholera (13.7%) was observed in 0-10 yr age group. Of them, 11.5 per cent belonged to 0-5 yr age group. V. cholerae O1 El Tor serotype Ogawa was the predominant isolate. Multiple drug-resistant isolates of V. cholerae O1 Ogawa were reported in the study. Emergence of resistance amongst V. cholerae towards many antibiotics is a matter of concern. Hence, continuous surveillance for diarrhoeal disorders is necessary to control the future outbreaks of cholera in this region.

Detection and isolation of the "Top 7" Shiga toxin-producing Escherichia coli in ground beef: comparison of the Rapidfinder kits to the USDA microbiology laboratory guidebook method

Science.gov (United States)

Shiga toxin-producing E. coli (STEC) O157:H7 and serogroups O26, O45, O103, O111, O121, and O145 are often referred to as the “top 7” STEC, and these have been declared as adulterants in beef by the USDA Food Safety and Inspection Service (FSIS). The aim of this work was to compare the methods des...

Cross-sectional study to estimate the prevalence of enterohaemorrhagic Escherichia coli on hides of market beef cows at harvest.

Science.gov (United States)

Schneider, L G; Stromberg, Z R; Lewis, G L; Moxley, R A; Smith, D R

2018-03-25

Cattle hides are an important source of enterohaemorrhagic Escherichia coli (EHEC) carcass contamination at slaughter. Seven EHEC serogroups are adulterants in raw, non-intact beef: EHEC O26, O45, O103, O111, O121, O145 and O157. The objective of this study was to estimate the probability for hide contamination with EHEC among US market beef cows at slaughter and to test the effects of season and geographic region on prevalence of hide contamination. Hides (n = 800) of market cows were swabbed at slaughter immediately after exsanguination, prior to hide removal. Cows were sampled from two geographically distinct beef packing plants during four seasons of 2015. Cattle source was categorized by northern or southern region. Samples were tested for EHEC by a molecular screening assay. The effects of region, season and their interaction on the probability of hide contamination by each EHEC serogroup were tested in separate multilevel multivariable logistic regression models, accounting for the random effect of clustering by plant. Statistical significance was set α = .05. Of 800 total samples, at least one EHEC was detected on 630 (79%) hides. Enterohaemorrhagic E. coli O26 was detected on 129 (16%) of all hides sampled, EHEC O45 on 437 (55%), EHEC O103 on 289 (36%), EHEC O111 on 189 (24%), EHEC O121 on 140 (18%), EHEC O145 on 171 (21%) and EHEC O157 on 89 (11%). Detection of EHEC O26 and EHEC O121 was associated with season. Season and region were associated with detecting EHEC O45 and EHEC O157. Season-by-region interactions were associated with the outcome of detecting EHEC O103, EHEC O111 and EHEC O145. Season, region of origin and the interaction of these factors affect hide contamination of market beef cattle at slaughter by EHEC, and each serogroup responds to these factors uniquely. © 2018 Blackwell Verlag GmbH.

26 CFR 301.6501(o)-3 - Partnership items.

Science.gov (United States)

2010-04-01

... 26 Internal Revenue 18 2010-04-01 2010-04-01 false Partnership items. 301.6501(o)-3 Section 301... § 301.6501(o)-3 Partnership items. (a) Partnership item defined. For purposes of section 6501(o) (as it..., and § 301.6511(g)-1, the term “partnership item” means— (1) Any item required to be taken into account...

Escherichia coli O26 IN RAW BUFFALO MILK: PRELIMINARY RESULTS

Directory of Open Access Journals (Sweden)

A. Rella

2013-02-01

Full Text Available Escherichia coli O26 is considered to be one of the most important food-borne pathogen. In this study, 120 buffalo milk samples collected in Lazio and in Apulia regions were tested for the presence of E. coli O26. One buffalo milk sample (0,8% tested positive for E. coli O26; the isolate was positive at the verocytotoxicity test and it showed resistance properties to different antimicrobial classes. These preliminary results highlight the need to monitor the foods of animal origin used for production and eaten by a wide range of persons, respect VTEC organism.

Poly[(6-carboxypicolinato-κ3O2,N,O6(μ3-pyridine-2,6-dicarboxylato-κ5O2,N,O6:O2′:O6′dysprosium(III

Directory of Open Access Journals (Sweden)

Xu Li

2009-11-01

Full Text Available In the title complex, [Dy(C7H3NO4(C7H4NO4]n, one of the ligands is fully deprotonated while the second has lost only one H atom. Each DyIII ion is coordinated by six O atoms and two N atoms from two pyridine-2,6-dicarboxylate and two 6-carboxypicolinate ligands, displaying a bicapped trigonal-prismatic geometry. The average Dy—O bond distance is 2.40 Å, some 0.1Å longer than the corresponding Ho—O distance in the isotypic holmium complex. Adjacent DyIII ions are linked by the pyridine-2,6-dicarboxylate ligands, forming a layer in (100. These layers are further connected by π–π stacking interactions between neighboring pyridyl rings [centroid–centroid distance = 3.827 (3 Å] and C—H...O hydrogen-bonding interactions, assembling a three-dimensional supramolecular network. Within each layer, there are other π–π stacking interactions between neighboring pyridyl rings [centroid–centroid distance = 3.501 (2 Å] and O—H...O and C—H...O hydrogen-bonding interactions, which further stabilize the structure.

Aqua(4-hydroxypyridine-2,6-dicarboxylato(1,10-phenanothrolinecopper(II 4.5-hydrate

Directory of Open Access Journals (Sweden)

Hossein Aghabozorg

2008-01-01

Full Text Available The title compound, [Cu(C7H3NO5(C12H8N2(H2O]·4.5H2O or [Cu(hypydc(phen(H2O]·4.5H2O (phen is 1,10-phenanthroline and hypydcH2 is 4-hydroxypyridine-2,6-dicarboxylic acid, was obtained by the reaction of copper(II nitrate hexahydrate with the proton-transfer compound (phenH2(hypydc in aqueous solution. Both the cationic and the anionic fragments of the proton-transfer compound are involved in complexation. Each CuII atom has a distorted octahedral geometry. It is hexacoordinated by three O atoms and three N atoms, from one phen fragment (as bidentate ligand, one (hypydc2− unit (as tridentate ligand and a water molecule. In the crystal structure, O—H...O and C—H...O hydrogen bonds, and π–π stacking interactions [centroid-to-centroid distance 3.5642 (11 Å] between the phen ring systems, contribute to the formation of a three-dimensional supramolecular structure.

Prevalent serogroups and antibiotic sensitivity of Neisseria gonorrhoeae

Directory of Open Access Journals (Sweden)

Aggarwal S

1992-01-01

Full Text Available One hundred and thirty two cases clinically labeled as acute gonorrhoea were investigated for gonococcal etiology. Smears were positive in 110 (83.3% cases and among these N. gonorrhoeae could be identified in 102 (77.3% cases by culture method. Strains were examined for serogrouping by monoclonal GC test which utilizes the principle of co-agglutination and detects the antigens of outer membrane protein. 96(94.1% strains belonged to serogroup W II/III, showing it to be the major serogroup circulating in the community. The strains were tested for sensitivity against 7 antibiotics. The largest proportion (30.4% of strains were resistant to penicillin (MIC>O. 125 IU/ml. Resistance to cotrimoxazole, erythromycin, cephalaxin and tetracycline was noted as 18.6, 17.6, 7.8 and 5.8 percent respectively. Strains showing resistance concurrently to two or more drugs were observed. All restrains were sensitive to gentamicin and norfloxacin. None of the strains was penicillinase producer.

Beyond the neutron drip line: The unbound oxygen isotopes ²⁵O and ²⁶O

DEFF Research Database (Denmark)

Caesar, C.; Simonis, J.; Adachi, T.

2013-01-01

The very neutron-rich oxygen isotopes 25O and 26O are investigated experimentally and theoretically. The unbound states are populated in an experiment performed at the R3B-LAND setup at GSI via proton-knockout reactions from 26F and 27F at relativistic energies around 442 and 414 MeV/nucleon, res...

Investigação genética da surdez hereditária: mutação do gene da Conexina 26 Genetic investigation of hereditary deafness: connexin 26 gene mutation

Directory of Open Access Journals (Sweden)

Paula Michele da Silva Schmidt

2009-01-01

Full Text Available Nos últimos anos houve grande progresso na localização de genes associados à deficiência auditiva hereditária, possibilitando diagnósticos cada vez mais precisos e precoces. Mutações no gene da Conexina 26 (GJB2 - Cx26 causam deficiência auditiva. Pela facilidade e benefício do rastreamento de mutações no gene GJB2, o teste genético está se tornando um importante recurso na saúde pública. O objetivo foi realizar pesquisa bibliográfica sobre a mutação do gene da Conexina 26 e sua influência na audição. Foi realizado um levantamento bibliográfico por meio de busca eletrônica utilizando os descritores: perda auditiva, genética, triagem genética, Conexina 26, nas bases de dados MEDLINE, SciELO e LILACS, desde a década de 90 até os dias atuais. Concluiu-se que a mutação 35delG da Conexina 26 está potencialmente vinculada a alguns casos de perda auditiva não esclarecida. A pesquisa desta mutação poderia ser incluída na bateria de exames de investigação etiológica da surdez indeterminada, uma vez que esclarece a etiologia de alguns casos e a sua identificação possibilita o aconselhamento genético.In the last few years, great progress has been made in the search for genes associated to hereditary hearing impairment, allowing more precise and earlier diagnosis. Connexin 26 gene mutations (GJB2 - Cx26 cause hearing impairment. Due to the easiness and benefits of the screening of mutations on the gene GJB2, genetic testing is becoming an important resource in public health. The aim of the present study was to conduct a literature research about the mutation of the Connexin 26 gene and its influence in hearing. It was carried out a literature review through electronic search using the keywords: hearing loss, genetics, genetic screening, and Connexin 26, at the databases MEDLINE, SciELO and LILACS, from the 90s to the present days. The results indicate that the 35delG mutation of Connexin 26 is potentially associated

Effect of severe weather events on the shedding of Shiga toxigenic Escherichia coli in slaughter cattle and phenotype of serogroup O157 isolates.

Science.gov (United States)

Stanford, Kim; Reuter, Tim; Bach, Susan J; Chui, Linda; Ma, Angela; Conrad, Cheyenne C; Tostes, Renata; McAllister, Tim A

2017-09-01

High-event periods (HEPs) occur sporadically when beef carcasses and meat have episodes of acute contamination with Shiga toxin-producing Escherichia coli (STEC). In this study, severe weather events were investigated as catalysts for HEPs based on PCR and isolate prevalence of seven E. coli serogroups in slaughter cattle feces. Winter ambient temperatures with daily means 10.5oC warmer or 12.3°C colder than seasonal norms (-10.4°C) most altered STEC shedding. Fecal samples yielded increased proportions (P  10 min and one also had strong biofilm-forming potential. However, this isolate lacked eae and stx genes. Severe weather can influence STEC shedding, particularly of O157, and could possibly trigger HEPs. However, our data suggest that it is unlikely for isolates to carry virulence genes and possess phenotypes capable of evading post-harvest microbiological interventions. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

YERSINIA PSEUDOTUBERCULOSIS, SEROGROUP O:1A, INFECTION IN TWO AMAZON PARROTS (AMAZONA AESTIVA AND AMAZONA ORATRIX) WITH HEPATIC HEMOSIDEROSIS.

Science.gov (United States)

Galosi, Livio; Farneti, Silvana; Rossi, Giacomo; Cork, Susan Catherine; Ferraro, Stefano; Magi, Gian Enrico; Petrini, Stefano; Valiani, Andrea; Cuteri, Vincenzo; Attili, Anna-Rita

2015-09-01

Necropsies were conducted on a female blue-fronted Amazon (Amazona aestiva) and a female yellow-headed Amazon (Amazona oratrix) that died after depression, ruffled feathers, diarrhea, and biliverdin in the urine. Gross and microscopic examinations revealed multifocal necrosis in the liver, spleen, lungs, kidneys, intestines, and heart caused by acute bacteremia. Yersinia pseudotuberculosis, serogroup O:1a, was isolated by culturing from the visceral lesions in the liver, intestines, and spleen. Virulence gene analysis showed the presence of the inv gene and the complete pathogenicity island: IS100, psn, yptE, irp1, irp2 ybtP-ybtQ, ybtX-ybtS, and int asnT-Int. Histopathologic findings and chemical analysis also demonstrated hepatic hemosiderosis. As has been demonstrated in other species, hemosiderosis may predispose Amazona spp. to systemic infection with Y. pseudotuberculosis after enteric disease.

Biofilm formation, antimicrobial susceptibility, serogroups and virulence genes of uropathogenic E. coli isolated from clinical samples in Iran

Directory of Open Access Journals (Sweden)

Elahe Tajbakhsh

2016-04-01

Full Text Available Abstract Background Uropathogenic Escherichia coli O- Serogroups with their virulence factors are the most prevalent causes of UTIs. The present research performed to track common uropathogenic E.coli serogroups, antibiotic resistance pattern of strains and prevalence of virulence genes in isolations having the ability to constitute biofilm. Methods In this research 130 E.coli isolation from patients having UTI symptoms were collected and antimicrobial resistance pattern was performed by Kirby-Bauer method. Polymerase chain reaction was done using primer pairs to identify common serogroups of uropathogenic E.coli and studying virulence genes in isolations creating biofilm. Results Among 130 E.coli isolates, 80 (61.53 % were able to make biofilm that 15 isolates (18.75 % indicated strong reaction, 20 (25 % of medium and 45 (56.25 % of weak biofilm reaction. Among isolations creating biofilm, the highest resistance reported to Ampicillin (87.5 % and the lowest to Nitrofurantoin (3.75 %. The frequency of fimH, pap, sfa and afa genes in isolations having the ability to create strong biofilm reported 93.33 %, 86.66 %, 86.66 % and 66.66 %, respectively. Conclusions The findings indicated the importance of virulence genes in serogroups producing uropathogenic E.coli biofilm. It is recommended that strains producing biofilm before antibiotic use should be studied.

Outbreak of Vibrio cholerae serogroup O1, serotype Ogawa, biotype El Tor strain--La Huasteca Region, Mexico, 2013.

Science.gov (United States)

Díaz-Quiñonez, Alberto; Hernández-Monroy, Irma; Montes-Colima, Norma; Moreno-Pérez, Asunción; Galicia-Nicolás, Adriana; Martínez-Rojano, Hugo; Carmona-Ramos, Concepción; Sánchez-Mendoza, Miroslava; Rodríguez-Martínez, José Cruz; Suárez-Idueta, Lorena; Jiménez-Corona, María Eugenia; Ruiz-Matus, Cuitláhuac; Kuri-Morales, Pablo

2014-06-27

On September 2 and 6, 2013, Mexico's National System of Epidemiological Surveillance identified two cases of cholera in Mexico City. Rectal swab cultures from both patients were confirmed as toxigenic Vibrio cholerae serogroup O1, serotype Ogawa, biotype El Tor. Pulsed-field gel electrophoresis and virulence gene amplification (ctxA, ctxB, zot, and ace) demonstrated that the strains were identical to one another but different from strains circulating in Mexico previously. The strains were indistinguishable from the strain that has caused outbreaks in Haiti, the Dominican Republic, and Cuba. The strain was susceptible to doxycycline, had intermediate susceptibility to ampicillin and chloramphenicol, was less than fully susceptible to ciprofloxacin, and was resistant to furazolidone and trimethoprim-sulfamethoxazole. An investigation failed to identify a common source of infection, additional cases, or any epidemiologic link between the cases. Both patients were treated with a single, 300-mg dose of doxycycline, and their symptoms resolved.

Isolation and characterization of non-O157 Shiga toxin-producing Escherichia coli from beef carcasses, cuts and trimmings of abattoirs in Argentina

Science.gov (United States)

Brusa, Victoria; Restovich, Viviana; Galli, Lucía; Teitelbaum, David; Signorini, Marcelo; Brasesco, Hebe; Londero, Alejandra; García, Diego; Padola, Nora Lía; Superno, Valeria; Sanz, Marcelo; Petroli, Sandra; Costa, Magdalena; Bruzzone, Mariana; Sucari, Adriana; Ferreghini, Marcela; Linares, Luciano; Suberbie, Germán; Rodríguez, Ricardo

2017-01-01

Several foods contaminated with Shiga toxin-producing Escherichia coli (STEC) are associated with human diseases. Some countries have established microbiological criteria for non-O157 STEC, thus, the absence of serogroups O26, O45, O103, O104, O111, O121, and O145 in sprouts from the European Union or ground beef and beef trimmings from the United States is mandatory. While in Argentina screening for O26, O103, O111, O145 and O121 in ground beef, ready-to-eat food, sausages and vegetables is mandatory, other countries have zero-tolerance for all STEC in chilled beef. The aim of this study was to provide data on the prevalence of non-O157 STEC isolated from beef processed in eight Argentinean cattle slaughterhouses producing beef for export and local markets, and to know the non-O157 STEC profiles through strain characterization and genotypic analysis. Samples (n = 15,965) from 3,205 beef carcasses, 9,570 cuts and 3,190 trimmings collected between March and September 2014 were processed in pools of five samples each. Pools of samples (n = 3,193) from 641 carcasses, 1,914 cuts and 638 trimming were analyzed for non-O157 STEC isolation according to ISO/CEN 13136:2012. Of these, 37 pools of carcasses (5.8%), 111 pools of cuts (5.8%) and 45 pools of trimmings (7.0%) were positive for non-O157 STEC. STEC strains (n = 200) were isolated from 193 pools of samples. The most prevalent serotypes were O174:H21, O185:H7, O8:H19, O178:H19 and O130:H11, and the most prevalent genotypes were stx2c(vh-b) and stx2a/saa/ehxA. O103:H21 strain was eae-positive and one O178:H19 strain was aggR/aaiC-positive. The prevalence of non-O157 STEC in beef carcasses reported here was low. None of the non-O157 STEC strains isolated corresponded to the non-O157 STEC serotypes and virulence profiles isolated from human cases in Argentina in the same study period. The application of microbiological criteria for each foodstuff should be determined by risk analysis in order to have a stringent

Isolation and characterization of non-O157 Shiga toxin-producing Escherichia coli from beef carcasses, cuts and trimmings of abattoirs in Argentina.

Directory of Open Access Journals (Sweden)

Victoria Brusa

Full Text Available Several foods contaminated with Shiga toxin-producing Escherichia coli (STEC are associated with human diseases. Some countries have established microbiological criteria for non-O157 STEC, thus, the absence of serogroups O26, O45, O103, O104, O111, O121, and O145 in sprouts from the European Union or ground beef and beef trimmings from the United States is mandatory. While in Argentina screening for O26, O103, O111, O145 and O121 in ground beef, ready-to-eat food, sausages and vegetables is mandatory, other countries have zero-tolerance for all STEC in chilled beef. The aim of this study was to provide data on the prevalence of non-O157 STEC isolated from beef processed in eight Argentinean cattle slaughterhouses producing beef for export and local markets, and to know the non-O157 STEC profiles through strain characterization and genotypic analysis. Samples (n = 15,965 from 3,205 beef carcasses, 9,570 cuts and 3,190 trimmings collected between March and September 2014 were processed in pools of five samples each. Pools of samples (n = 3,193 from 641 carcasses, 1,914 cuts and 638 trimming were analyzed for non-O157 STEC isolation according to ISO/CEN 13136:2012. Of these, 37 pools of carcasses (5.8%, 111 pools of cuts (5.8% and 45 pools of trimmings (7.0% were positive for non-O157 STEC. STEC strains (n = 200 were isolated from 193 pools of samples. The most prevalent serotypes were O174:H21, O185:H7, O8:H19, O178:H19 and O130:H11, and the most prevalent genotypes were stx2c(vh-b and stx2a/saa/ehxA. O103:H21 strain was eae-positive and one O178:H19 strain was aggR/aaiC-positive. The prevalence of non-O157 STEC in beef carcasses reported here was low. None of the non-O157 STEC strains isolated corresponded to the non-O157 STEC serotypes and virulence profiles isolated from human cases in Argentina in the same study period. The application of microbiological criteria for each foodstuff should be determined by risk analysis in order to have a

Prevalence of Avian Pathogenic Escherichia coli (APEC Clone Harboring sfa Gene in Brazil

Directory of Open Access Journals (Sweden)

Terezinha Knöbl

2012-01-01

Full Text Available Escherichia coli sfa+ strains isolated from poultry were serotyped and characterized by polymerase chain reaction (PCR and amplified fragment length polymorphism (AFLP. Isolates collected from 12 Brazilian poultry farms mostly belonged to serogroup O6, followed by serogroups O2, O8, O21, O46, O78, O88, O106, O111, and O143. Virulence genes associated were: iuc 90%, fim 86% neuS 60%, hly 34%, tsh 28%, crl/csg 26%, iss 26%, pap 18%, and 14% cnf. Strains from the same farm presented more than one genotypic pattern belonging to different profiles in AFLP. AFLP showed a clonal relation between Escherichia coli sfa+ serogroup O6. The virulence genes found in these strains reveal some similarity with extraintestinal E. coli (ExPEC, thus alerting for potential zoonotic risk.

Optimized enrichment for the detection of Escherichia coli O26 in French raw milk cheeses.

Science.gov (United States)

Savoye, F; Rozand, C; Bouvier, M; Gleizal, A; Thevenot, D

2011-06-01

Our main objective was to optimize the enrichment of Escherichia coli O26 in raw milk cheeses for their subsequent detection with a new automated immunological method. Ten enrichment broths were tested for the detection of E. coli O26. Two categories of experimentally inoculated raw milk cheeses, semi-hard uncooked cheese and 'Camembert' type cheese, were initially used to investigate the relative efficacy of the different enrichments. The enrichments that were considered optimal for the growth of E. coli O26 in these cheeses were then challenged with other types of raw milk cheeses. Buffered peptone water supplemented with cefixim-tellurite and acriflavin was shown to optimize the growth of E. coli O26 artificially inoculated in the cheeses tested. Despite the low inoculum level (1-10 CFU per 25 g) in the cheeses, E. coli O26 counts reached at least 5.10(4) CFU ml(-1) after 24-h incubation at 41.5 °C in this medium. All the experimentally inoculated cheeses were found positive by the immunological method in the enrichment broth selected. Optimized E. coli O26 enrichment and rapid detection constitute the first steps of a complete procedure that could be used in routine to detect E. coli O26 in raw milk cheeses. © 2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology.

Synthesis of ceramic powders of La9,56 (SiO4)6O2,34 and La9,8Si5,7MgO,3O26,4 by modified sol-gel process

International Nuclear Information System (INIS)

Lira, Sabrina Lopes; Paiva, Mayara Rafaela Soares; Misso, Agatha Matos; Elias, Daniel Ricco; Yamagata, Chieko

2012-01-01

Lanthanum silicate oxyapatite materials are promising for application as electrolyte in solid oxide fuel cells because of high ionic conductivity at temperatures between 600 deg C and 800 deg C. In this work, oxyapatites with the composition La 9,56 (SiO 4 ) 6 O 2,34 , and La 9,8 Si 5,7 Mg 0,3 O 26,4 were synthesized by using the sol-gel method, followed by precipitation. Initially, the gel of silica was synthesized from sodium silicate solution, by acid catalysis using lanthanum and magnesium chloride solution. Then, the La and Mg hydroxides were precipitated with NaOH in the gel. The powders were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and measurements of specific surface area. The crystalline oxyapatite phase of La 9,56 (SiO 4 ) 6 O 2,34 , and was La 9,8 Si 5,7 Mg 0,3 O 26,4 obtained by calcination at 900 deg C for 2 and 1h respectively (author)

Enterobacterial repetitive intergenic consensus sequences and the PCR to generate fingerprints of genomic DNAs from Vibrio cholerae O1, O139, and non-O1 strains.

OpenAIRE

Rivera, I G; Chowdhury, M A; Huq, A; Jacobs, D; Martins, M T; Colwell, R R

1995-01-01

Enterobacterial repetitive intergenic consensus (ERIC) sequence polymorphism was studied in Vibrio Cholerae strains isolated before and after the cholera epidemic in Brazil (in 1991), along with epidemic strains from Peru, Mexico, and India, by PCR. A total of 17 fingerprint patterns (FPs) were detected in the V. cholerae strains examined; 96.7% of the toxigenic V. cholerae O1 strains and 100% of the O139 serogroup strains were found to belong to the same FP group comprising four fragments (F...

Differentiating non-0157:H7 STEC serogroups from ground beef plated on agar media by hyperspetral imaging

Science.gov (United States)

Introduction: The development of an assay to detect and confirm a positive non-O157:H7 isolate is challenging when mixed morphologically results are obtained from the serogroups growing on Rainbow agar. Rainbow agar is only claimed by the manufacturer to be very specific for E.coli O157:H7 strain...

Deterioração controlada para avaliar o potencial fisiológico de sementes de beterraba Controlled deterioration to evaluate the physiological potential of beetroot seeds

Directory of Open Access Journals (Sweden)

Josué B Silva

2012-09-01

Full Text Available Sementes de hortaliças quase sempre apresentam elevado valor comercial, motivo pelo qual devem ter o potencial fisiológico eficientemente avaliado. O teste de deterioração controlada é um dos recomendados para avaliação do vigor de sementes pequenas, como as de beterraba. O objetivo do trabalho foi determinar o melhor procedimento para a condução de referido teste em sementes de beterraba e sua relação com a emergência de plântulas em campo. Foram utilizados sete lotes de sementes da cultivar Top Tall Early Wonder, adquiridos no comércio. Após lavagem em água corrente, as sementes foram submetidas aos testes de germinação, velocidade de germinação, envelhecimento acelerado, emergência de plântulas em campo, velocidade de emergência e deterioração controlada (temperaturas de 41 e 45ºC, teores de água de 22, 24 e 26% e períodos de exposição de 12, 24 e 36 horas. As combinações 41ºC/24%/12 h, 41ºC/24%/36 h, 41ºC/26%/36 h e 45ºC/24%/24 h apresentaram potencial para uso na avaliação do vigor de sementes de beterraba. No entanto, sob as combinações de 41°C/24%/12 h ou 45°C/24%/24 h reduziu-se o tempo de condução do teste.Seeds of vegetable crop species always present high commercial value, so the physiological potential must be efficiently evaluated. The controlled deterioration is a recommended test to evaluate seed vigor of small-seeded vegetable crops, such as beetroot seeds. This research was run in order to determine the best controlled deterioration test procedures for beetroot seeds and its relationship with field seedling emergence. Seven commercial seed lots of the cultivar Top Tall Early Wonder were used. After washing in running water, the seeds were tested for germination, speed of germination, accelerated aging, seedling field emergence, emergence rate and controlled deterioration (41 and 45°C, moisture contents of 22, 24 and 26% and exposure times of 12, 24 and 36 hours. The combination of 41�

Enterobacterial repetitive intergenic consensus sequences and the PCR to generate fingerprints of genomic DNAs from Vibrio cholerae O1, O139, and non-O1 strains.

Science.gov (United States)

Rivera, I G; Chowdhury, M A; Huq, A; Jacobs, D; Martins, M T; Colwell, R R

1995-08-01

Enterobacterial repetitive intergenic consensus (ERIC) sequence polymorphism was studied in Vibrio Cholerae strains isolated before and after the cholera epidemic in Brazil (in 1991), along with epidemic strains from Peru, Mexico, and India, by PCR. A total of 17 fingerprint patterns (FPs) were detected in the V. cholerae strains examined; 96.7% of the toxigenic V. cholerae O1 strains and 100% of the O139 serogroup strains were found to belong to the same FP group comprising four fragments (FP1). The nontoxigenic V. cholerae O1 also yielded four fragments but constituted a different FP group (FP2). A total of 15 different patterns were observed among the V. cholerae non-O1 strains. Two patterns were observed most frequently for V. cholerae non-01 strains, 25% of which have FP3, with five fragments, and 16.7% of which have FP4, with two fragments. Three fragments, 1.75, 0.79, and 0.5 kb, were found to be common to both toxigenic and nontoxigenic V. cholerae O1 strains as well as to group FP3, containing V. cholerae non-O1 strains. Two fragments of group FP3, 1.3 and 1.0 kb, were present in FP1 and FP2 respectively. The 0.5-kb fragment was common to all strains and serogroups of V. cholerae analyzed. It is concluded from the results of this study, based on DNA FPs of environmental isolates, that it is possible to detect an emerging virulent strain in a cholera-endemic region. ERIC-PCR constitutes a powerful tool for determination of the virulence potential of V. cholerae O1 strains isolated in surveillance programs and for molecular epidemiological investigations.

Phase relations, crystal structure, and phase transformation of In_1_−_xNb_1_−_xTi_2_xO_4 (0 ≤ x < 0.45) in In_2O_3–Nb_2O_5–TiO_2 system

International Nuclear Information System (INIS)

Su, Liumei; Fan, Xing; Cai, Gemei; Liu, Huashan; Jin, Zhanpeng

2015-01-01

Phase relations, crystal structures, and phase transformation of In_1_−_xNb_1_−_xTi_2_xO_4 (0 ≤ x < 0.45) in In_2O_3–Nb_2O_5–TiO_2 ternary system were investigated for the first time. A number of samples with different compositions were prepared by a solid-state reaction method, and phase assembles were analyzed by powder X-ray diffraction (XRD), scanning electron microscopy (SEM), and electron probe micro-analysis (EPMA). Five three-phase regions, ten two-phase regions, and six single-phase solid solutions were determined in this system. The solid solution of In_1_−_xNb_1_−_xTi_2_xO_4 (0 ≤ x < 0.45) is composed of both ordered monoclinic wolframite-type structure (0 ≤ x < 0.35) and disordered orthorhombic α-PbO_2 type structure (0.35 < x < 0.45). Driving force for composition-driven phase transformation in In_1_−_xNb_1_−_xTi_2_xO_4 (0 ≤ x < 0.45) stems from the ordering of cations. The ever reported compound InNbTiO_6 with an orthorhombic α-PbO_2 type structure was amended to be a monoclinic wolframite-type structure. Present investigations will be useful for the whole ceramic community working with In_2O_3–Nb_2O_5–TiO_2 ternary system as well as for the development of functional materials. - Highlights: • Phase relations of In_2O_3–Nb_2O_5–TiO_2 ternary system were constructed. • Crystal structures of a novel solid solution In_1_−_xNb_1_−_xTi_2_xO_4 were determined. • Crystal structure of InNbTiO_6 was amended to be a wolframite-type structure. • Composition-driven phase transformation of In_1_−_xNb_1_−_xTi_2_xO_4 was investigated.

[Virulence markers of Escherichia coli O1 strains].

Science.gov (United States)

Makarova, M A; Kaftyreva, L A; Grigor'eva, N S; Kicha, E V; Lipatova, L A

2011-01-01

To detect virulence genes in clinical isolates of Escherichia coli O1 using polymerase chain reaction (PCR). One hundred and twenty strains of E.coli O1 strains isolated from faeces of patients with acute diarrhea (n = 45) and healthy persons (n = 75) were studied. PCR with primers for rfb and fliC genes, which control synthesis of O- and H- antigens respectively, was used. Fourteen virulence genes (pap, aaf, sfa, afa, eaeA, bfpA, ial, hly, cnf, stx1, stx2, lt, st, and aer) were detected by PCR primers. K1-antigen was determined by Pastorex Meningo B/E. coli O1 kit (Bio-Rad). rfb gene controlling O-antigen synthesis in serogroup O1 as well as fliC gene controlling synthesis of H7 and K1 antigens were detected in all strains. Thus all E. coli strains had antigenic structure O1:K1 :H-:F7. Virulence genes aafl, sfa, afa, eaeA, bfpA, ial, hly, cnf, stx1, stx2, lt, and st were not detected. All strains owned pap and aer genes regardless of the presence of acute diarrhea symptoms. It was shown that E. coli O1:KI:H-:F7 strains do not have virulence genes which are characteristic for diarrhea-causing Escherichia. In accordance with the presence of pap and aer genes they could be attributed to uropathogenic Escherichia (UPEC) or avian-pathogenic Escherichia (APEC). It is necessary to detect virulence factors in order to determine E. coli as a cause of intestinal infection.

Optical properties of BaO added bioactive Na2O-CaO-P2O5 glasses

Science.gov (United States)

Edathazhe, Akhila B.; Shashikala, H. D.

2018-04-01

This paper deals with the effect of BaO addition on the optical properties of bioactive Na2O-CaO-P2O5 glasses for biomedical optics applications. The phosphate glasses with composition (26-x)Na2O-xBaO-29CaO-45P2O5 (x = 0, 5, 10, 15 mol%) have been prepared by melt-quenching technique at 1100°C. The refractive index of glasses increased with BaO content. The optical band gap and Urbach energy of synthesized glasses were derived from the optical absorption spectra by using UV-Visible spectrometer. The addition of 5 mol% of BaO increased the band gap energy of glasses due to the formation of ionic cross-links in the glass structure. The defect and interstitial bonds formation in theglasses decreased with BaO additions as indicated by reductions in the Urbach energy values. No such variations in the band gap and Urbach energy values of glasses were observed with BaO content from 5 to 15 mol%. The molar and oxide ion polarizability values were calculated from the band gap and molar volume of glasses. The increase in the calculated optical basicity and metallization criteria of glasses supported the rise in band gap energy values with BaO additions. As the melting temperature of glasses decreased from 1200 to 1100°C, the refractive index increased as supported by the measured density values. The band gap energy is not changed with melting temperature. The Urbach energy decreased with decrease in melting temperature in case of BaO-free Na2O-CaO-P2O5 glasses, whereas it increased in case of BaO added glasses due to the role of BaO as modifying oxide.

Sequence Variations in the Flagellar Antigen Genes fliCH25 and fliCH28 of Escherichia coli and Their Use in Identification and Characterization of Enterohemorrhagic E. coli (EHEC O145:H25 and O145:H28.

Directory of Open Access Journals (Sweden)

Lothar Beutin

Full Text Available Enterohemorrhagic E. coli (EHEC serogroup O145 is regarded as one of the major EHEC serogroups involved in severe infections in humans. EHEC O145 encompasses motile and non-motile strains of serotypes O145:H25 and O145:H28. Sequencing the fliC-genes associated with the flagellar antigens H25 and H28 revealed the genetic diversity of the fliCH25 and fliCH28 gene sequences in E. coli. Based on allele discrimination of these fliC-genes real-time PCR tests were designed for identification of EHEC O145:H25 and O145:H28. The fliCH25 genes present in O145:H25 were found to be very similar to those present in E. coli serogroups O2, O100, O165, O172 and O177 pointing to their common evolution but were different from fliCH25 genes of a multiple number of other E. coli serotypes. In a similar way, EHEC O145:H28 harbor a characteristic fliCH28 allele which, apart from EHEC O145:H28, was only found in enteropathogenic (EPEC O28:H28 strains that shared some common traits with EHEC O145:H28. The real time PCR-assays targeting these fliCH25[O145] and fliCH28[O145] alleles allow better characterization of EHEC O145:H25 and EHEC O145:H28. Evaluation of these PCR assays in spiked ready-to eat salad samples resulted in specific detection of both types of EHEC O145 strains even when low spiking levels of 1-10 cfu/g were used. Furthermore these PCR assays allowed identification of non-motile E. coli strains which are serologically not typable for their H-antigens. The combined use of O-antigen genotyping (O145wzy and detection of the respective fliCH25[O145] and fliCH28[O145] allele types contributes to improve identification and molecular serotyping of E. coli O145 isolates.

Use of Heavy Water (D2O in Developing Thermostable Recombinant p26 Protein Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Equine Infectious Anemia Virus Infection

Directory of Open Access Journals (Sweden)

Harisankar Singha

2014-01-01

Full Text Available Thermostabilizing effect of heavy water (D2O or deuterium oxide has been demonstrated previously on several enzymes and vaccines like oral poliovirus vaccine and influenza virus vaccine. In view of the above observations, effect of heavy water on in situ thermostabilization of recombinant p26 protein on enzyme-linked immunosorbent assay (ELISA for serodiagnosis of equine infectious anemia virus (EIAV infection was investigated in the present study. The carbonate-bicarbonate coating buffer was prepared in 60% and 80% D2O for coating the p26 protein in 96-well ELISA plate and thermal stability was examined at 4°C, 37°C, 42°C, and 45°C over a storage time from 2 weeks to 10 months. A set of positive serum (n=12 consisting of strong, medium, and weak titer strength (4 samples in each category and negative serum (n=30 were assessed in ELISA during the study period. At each time point, ELISA results were compared with fresh plate to assess thermal protective effect of D2O. Gradual increase in the stabilizing effect of 80% D2O at elevated temperature (37°C < 42°C < 45°C was observed. The 80% D2O provides the thermal protection to rp26 protein in ELISA plate up to 2 months of incubation at 45°C. The findings of the present study have the future implication of adopting cost effective strategies for generating more heat tolerable ELISA reagents with extended shelf life.

[Detection of putative polysaccharide biosynthesis genes in Azospirillum brasilense strains from serogroups I and II].

Science.gov (United States)

Petrova, L P; Prilipov, A G; Katsy, E I

2017-01-01

It is known that in Azospirillum brasilense strains Sp245 and SR75 included in serogroup I, the repeat units of their O-polysaccharides consist of five residues of D-rhamnose, and in strain SR15, of four; and the heteropolymeric O-polysaccharide of A. brasilense type strain Sp7 from serogroup II contains not less than five types of repeat units. In the present work, a complex of nondegenerate primers to the genes of A. brasilense Sp245 plasmids AZOBR_p6, AZOBR_p3, and AZOBR_p2, which encode putative enzymes for the biosynthesis of core oligosaccharide and O-polysaccharide of lipopolysaccharide, capsular polysaccharides, and exopolysaccharides, was proposed. By using the designed primers, products of the expected sizes were synthesized in polymerase chain reactions on genomic DNA of A. brasilense Sp245, SR75, SR15, and Sp7 in 36, 29, 23, and 12 cases, respectively. As a result of sequencing of a number of amplicons, a high (86–99%) level of identity of the corresponding putative polysaccharide biosynthesis genes in three A. brasilense strains from serogroup I was detected. In a blotting-hybridization reaction with the biotin-labeled DNA of the A. brasilense gene AZOBR_p60122 coding for putative permease of the ABC transporter of polysaccharides, localization of the homologous gene in ~120-MDa plasmids of the bacteria A. brasilense SR15 and SR75 was revealed.

Photocatalytic degradation of textile dye direct orange 26 by using CoFe2O4/Ag2O

International Nuclear Information System (INIS)

Azhdari, F.; Mehdipour Ghazi, M.

2016-01-01

The magnetic and recyclable nanoparticles of CoFe 2 O 4 were synthesized by a reverse co-precipitation process. Sonication was used to couple the CoFe 2 O 4 surface with Ag 2 O. The characteristics and optical properties of the catalyst were studied by powder X-ray diffraction, UV–visible reflectance spectroscopy and scanning electron microscopy analyses. Pure CoFe 2 O 4 and CoFe 2 O 4 /Ag 2 O were utilized to determine the visible light photo catalytic degradation of Direct Orange 26. The effects of p H, the initial concentration of catalyst and initial dye concentration on the photo catalytic process were investigated. It was found that the presence of Ag 2 O remarkably improved the photo catalytic adsorption capacity and degradation efficiency of CoFe 2 O 4 /Ag 2 O when compared with the pure CoFe 2 O 4 . Moreover, due to the magnetic behavior of CoFe 2 O 4 , these coupled nanoparticles can be easily separated from the aqueous solution by applying an external magnetic field. The prepared Ag 2 O-modified CoFe 2 O 4 exhibited much higher (about 40%) photo catalytic activity than the unmodified one. The results showed that the loading of the Ag 2 O significantly improved the photo catalytic performance of the CoFe 2 O 4 in which the Ag 2 O acted as a charge carrier to capture the delocalized electrons.

Fabrication of 45 degrees template grain boundary junctions using a CaO lift-off technique

NARCIS (Netherlands)

IJsselsteijn, R.P.J.; Terpstra, D.; Flokstra, Jakob; Rogalla, Horst

1994-01-01

45 degrees grain boundary junctions have been made using (100) MgO substrates, a CeO2 template layer and an YBa2Cu3O7 top layer. To minimize the damage to the MgO surface, which will occur if the CeO2 is structured using ion milling, the CeO2 layer has been structured using the CaO lift-off

OmpU as a biomarker for rapid discrimination between toxigenic and epidemic Vibrio cholerae O1/O139 and non-epidemic Vibrio cholerae in a modified MALDI-TOF MS assay

NARCIS (Netherlands)

Paauw, A.; Trip, H.; Niemcewicz, M.; Sellek, R.; Heng, J.M.E.; Mars-Groenendijk, R.H.; Jong, A.L. de; Majchrzykiewicz-Koehorst, J.A.; Olsen, J.S.; Tsivtsivadze, E.

2014-01-01

Background Cholera is an acute diarrheal disease caused by Vibrio cholerae. Outbreaks are caused by a genetically homogenous group of strains from serogroup O1 or O139 that are able to produce the cholera toxin. Rapid detection and identification of these epidemic strains is essential for an

Misidentification of Vibrio cholerae O155 isolated from imported shrimp as O serogroup O139 due to cross-agglutination with commercial O139 antisera

DEFF Research Database (Denmark)

Dalsgaard, A.; Mazur, J.; Dalsgaard, Inger

2002-01-01

. The strain contained two plasmids, in contrast to other O139 strains, which normally do not contain plasmids. The characteristics of the strain led to further agglutination testing with other antisera that are not commercially available, and the strain was found to agglutinate O155 antiserum in repeated...... was isolated from imported raw frozen shrimp. The toxigenicity of the strain was analyzed, and the results of a polymerase chain reaction showed that the V. cholerae strain did not contain the virulence genes ctx, tcp9, and zot, which are normally found in V. cholerae O1 and O139. The strain was resistant...... to colistin and spectinomycin. The high susceptibility of the strain to antimicrobial agents was confirmed by the lack of an SXT element, a self-transmissible, chromosomal genetic element that is normally present in O139 strains and encodes resistance to sulfonamides, trimethoprim, and streptomycin...

K4Nb6O17·4.5H2O: A novel dual functional material with quick photoreduction of Cr(VI) and high adsorptive capacity of Cr(III)

International Nuclear Information System (INIS)

Ma, Yuli; Liu, Xiaoqing; Li, Yang; Su, Yiguo; Chai, Zhanli; Wang, Xiaojing

2014-01-01

Graphical abstract: A well crystalline K 4 Nb 6 O 17 ·4.5H 2 O with a wide layer spacing possesses an excellent disposal performance for chromium species of Cr(VI) and Cr(III) as well as the superior recyclability due to its high stability and convenient regeneration process. - Highlights: • A nano-sheet K 4 Nb 6 O 17 ·4.5H 2 O with a large layer spacing was synthesized. • K 4 Nb 6 O 17 ·4.5H 2 O showed a superior photoreduction of Cr(VI) in an acidic solution. • The sample showed a high adsorption capacity of Cr(III) in a near neutral solution. • K 4 Nb 6 O 17 ·4.5H 2 O regenerated conveniently by immersing in a KOH solution. • A complete removal of chromium species was retained after recycling five times. - Abstract: A series of orthorhombic phase K 4 Nb 6 O 17 ·4.5H 2 O was synthesized via a hydrothermal approach. When presented in an acidic pH range, K 4 Nb 6 O 17 ·4.5H 2 O showed a strong ability in quick reduction from Cr(VI) to Cr(III). The resulted Cr(III) ions were removed by an effective adsorption through simply adjusting the solution pH from strong acidity to near neutrality, owing to the sample's unique nano-sheet structure with a wide layer spacing. The Cr(III) ions adsorbed onto samples were released again for reusing by eluting with 1 mol L −1 HCl solution, and K 4 Nb 6 O 17 ·4.5H 2 O regenerated by immersing in a KOH solution. The reduction efficiency of Cr(VI) was still up to 98% after irradiation for 60 min, and the removal efficiency of Cr(III) ions was as high as 83% even after five cycles. Therefore, K 4 Nb 6 O 17 ·4.5H 2 O is clearly demonstrated to be an excellent dual functional material with quick photoreduction of Cr(VI) and high adsorptive capacity of Cr(III). The relevant materials reported herein might be found various environment-related applications

4,5-Di-O-Caffeoylquinic Acid from Ligularia fischeri Suppresses Inflammatory Responses Through TRPV1 Activation.

Science.gov (United States)

Kim, Yiseul; Kim, Jung Tae; Park, Joonwoo; Son, Hee Jin; Kim, Eun-Young; Lee, Young Joo; Rhyu, Mee-Ra

2017-10-01

Ligularia fischeri (Ledeb.) Turcz., a perennial plant native to northeastern Asia, has long been used as folk remedies for the alleviation of inflammatory symptoms. We investigated whether the extract of L. fischeri (LFEx) and caffeoylquinic acid (CQA) derivatives, the pharmacologically active ingredients identified from L. fischeri, regulate inflammation via a transient receptor potential vanilloid 1 (TRPV1)-mediated pathway. Changes in intracellular Ca 2+ levels to the LFEx and trans-5-O-CQA, 3,4-di-O-CQA, 3,5-di-O-CQA, and 4,5-di-O-CQA were monitored in TRPV1-expressing human embryonic kidney cell HEK 293T. LFEx and 4,5-di-O-CQA (EC 50  = 69.34 ± 1.12 μM) activated TRPV1, and these activations were significantly inhibited by ruthenium red, a general blocker of TRP channels, and capsazepine, a specific antagonist of TRPV1. 4,5-Di-O-CQA has been determined having antiinflammatory effect under hypoxic conditions by detecting the expression of cyclooxygenase-2 (COX-2), a representative inflammatory marker, and cellular migration in human pulmonary epithelial A549 cells. 4,5-Di-O-CQA suppressed COX-2 expression and cell migration, and this inhibition was countered by co-treatment with capsazepine. This study provides evidence that L. fischeri is selective to inflammatory responses via a TRPV1-mediated pathway, and 4,5-di-O-CQA might play a key role to create these effects. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

Whole genome PCR scanning reveals the syntenic genome structure of toxigenic Vibrio cholerae strains in the O1/O139 population.

Directory of Open Access Journals (Sweden)

Bo Pang

Full Text Available Vibrio cholerae is commonly found in estuarine water systems. Toxigenic O1 and O139 V. cholerae strains have caused cholera epidemics and pandemics, whereas the nontoxigenic strains within these serogroups only occasionally lead to disease. To understand the differences in the genome and clonality between the toxigenic and nontoxigenic strains of V. cholerae serogroups O1 and O139, we employed a whole genome PCR scanning (WGPScanning method, an rrn operon-mediated fragment rearrangement analysis and comparative genomic hybridization (CGH to analyze the genome structure of different strains. WGPScanning in conjunction with CGH revealed that the genomic contents of the toxigenic strains were conservative, except for a few indels located mainly in mobile elements. Minor nucleotide variation in orthologous genes appeared to be the major difference between the toxigenic strains. rrn operon-mediated rearrangements were infrequent in El Tor toxigenic strains tested using I-CeuI digested pulsed-field gel electrophoresis (PFGE analysis and PCR analysis based on flanking sequence of rrn operons. Using these methods, we found that the genomic structures of toxigenic El Tor and O139 strains were syntenic. The nontoxigenic strains exhibited more extensive sequence variations, but toxin coregulated pilus positive (TCP+ strains had a similar structure. TCP+ nontoxigenic strains could be subdivided into multiple lineages according to the TCP type, suggesting the existence of complex intermediates in the evolution of toxigenic strains. The data indicate that toxigenic O1 El Tor and O139 strains were derived from a single lineage of intermediates from complex clones in the environment. The nontoxigenic strains with non-El Tor type TCP may yet evolve into new epidemic clones after attaining toxigenic attributes.

Butane-1,4-diammonium bis(pyridine-2,6-dicarboxylato-κ3O2,N,O6cadmate(II dihydrate

Directory of Open Access Journals (Sweden)

Najmeh Firoozi

2008-10-01

Full Text Available In the title compound, (C4H14N2[Cd(C7H3NO42]·2H2O, the CdII ion is coordinated by four O atoms [Cd—O = 2.2399 (17–2.2493 (17 Å] and two N atoms [Cd—N = 2.3113 (15 and 2.3917 (15 Å] from two tridentate pyridine-2,6-dicarboxylato ligands in a distorted octahedral geometry. The uncoordinated water molecules are involved in O—H...O and N—H...O hydrogen bonds, which contribute to the formation of a three-dimensional supramolecular structure, along with π–π stacking interactions [centroid–centroid distances of 3.5313 (13 and 3.6028 (11 Å between the pyridine rings of neighbouring dianions].

Study of 24Mg(18O,16O)26Mg reaction at Esub(180)=50MeV including scattering in entrance and exit channel

International Nuclear Information System (INIS)

Bernas, M.; Pougheon, F.; Roy-Stephan, M.; Berg, G.P.A.; Kernforschungsanlage Juelich G.m.b.H.; Berthier, B.; Le Fevre, J.P.; Wildenthal, B.H.

1980-01-01

Cross section angular distributions of 24 Mg( 18 O, 16 O) 26 Mg reaction at 50 MeV incident energy leading to the first four excited states in 26 Mg and the elastic and inelastic scattering for 18 O + 24 Mg at 50 MeV and 16 O + 26 Mg at 56 MeV have been measured in order to investigate the reaction mechanism of the two-nucleon transfer reaction including the analysis of incident and exit channels. The measured angular distributions were analyzed in the framework of the EFR-DWBA. Two neutron transfer amplitudes were determined using the 2s-1d shell model amplitudes calculated by Chung and Wildenthal. The large experimental cross section in contrast to the smallness of the direct transfer amplitude and the peculiar shape of the measured angular distribution of the lowest 2 1 + state suggests that this level is mainly excited by two-step processes. It is shown that a CCBA calculation describes successfully the angular distributions of the ground state and the 2 1 + state in 26 Mg

Propane-1,3-diammonium bis[aquachlorido(4-hydroxypyridine-2,6-dicarboxylato-κ3O2,N,O6mercurate(II] tetrahydrate

Directory of Open Access Journals (Sweden)

Hossein Aghabozorg

2008-08-01

Full Text Available The reaction of mercury(II chloride dihydrate, propane-1,3-diamine and 4-hydroxypyridine-2,6-dicarboxylic acid in a 1:1:1 molar ratio in aqueous solution, resulted in the formation of the title compound, (C3H12N2[Hg(C7H3NO5Cl(H2O]2·4H2O or (pnH2[Hg(hypydcCl(H2O]2·4H2O (where pn is propane-1,3-diamine and hypydcH2 is 4-hydroxypyridine-2,6-dicarboxylic acid. The metal atom is coordinated by one chloride group, one water molecule cis to the chloride ligand and one (hypydc2− ligand. The coordinated water molecule is almost perpendicular to the plane of the aromatic ring of (hypydc2−. The geometry of the resulting HgClNO3 coordination can be described as distorted square-pyramidal. This structure also contains propane-1,3-diammonium (site symmetry 2 as a counter-ion and four uncoordinated water molecules. There is a wide range of non-covalent interactions consisting of hydrogen bonding [of the types O—H...O, N—H...O and C—H...O, with D...A ranging from 2.548 (5 to 3.393 (6 Å] and ion pairing.

Water sources as reservoirs of Vibrio cholerae O1 and non-O1 strains in Bepanda, Douala (Cameroon): relationship between isolation and physico-chemical factors.

Science.gov (United States)

Akoachere, Jane-Francis Tatah Kihla; Mbuntcha, Christelle Kwedjeu Pulcherie

2014-07-30

Cholera has been endemic in Douala since 1971. Most outbreaks start from Bepanda, an overcrowded neighbourhood with poor hygiene and sanitary conditions. We investigated water sources in Bepanda as reservoirs of Vibrio cholerae, the causative agent of cholera, determined its antibiotic susceptibility and some physico-chemical characteristics that could maintain the endemicity of this organism in Bepanda. Three hundred and eighteen water samples collected from 45 wells, 8 taps and 1 stream from February to July 2009 were analyzed for V. cholerae using standard methods. Isolates were characterized morphologically, biochemically and serologically. The disc diffusion technique was employed to investigate antibiotic susceptibility. Differences in prevalence of organism between seasons were analysed. Correlation strength and direction of association between physico-chemical parameters and occurrence of V. cholerae was analyzed using the Kendall tau_b non-parametric correlation. This was further confirmed with the forward-stepwise binary logistic regression. Eighty-seven (27.4%) samples were positive for V. cholerae. Isolation was highest from wells. The organism was isolated in the rainy season and dry season but the frequency of isolation was significantly higher (χ2 = 7.009, df = 1, P = 0.008) in the rainy season. Of the 96 confirmed V. cholerae isolates, 32 (33.3%) belonged to serogroup O1 and 64 (66.6%) were serogroup non-O1/non-O139. Isolates from tap (municipal water) were non-O1/non-O139 strains. Salinity had a significant positive correlation with isolation in the dry season (+0.267, P = 0.015) and rainy season (+0.223, P = 0.028). The forward-stepwise method of binary logistic regression indicated that as pH (Wald = 11.753, df = 1), P = 0.001) increased, odds of isolation of V. cholerae also increased (B = 1.297, S.E = 0.378, Exp(B) = 3.657). All isolates were sensitive to ciprofloxacin and ofloxacin. Multi-drug resistance was predominant among the non-O1/non-O

Sequence Variations in the Flagellar Antigen Genes fliC H25 and fliC H28 of Escherichia coli and Their Use in Identification and Characterization of Enterohemorrhagic E. coli (EHEC) O145:H25 and O145:H28

Science.gov (United States)

Beutin, Lothar; Delannoy, Sabine; Fach, Patrick

2015-01-01

Enterohemorrhagic E. coli (EHEC) serogroup O145 is regarded as one of the major EHEC serogroups involved in severe infections in humans. EHEC O145 encompasses motile and non-motile strains of serotypes O145:H25 and O145:H28. Sequencing the fliC-genes associated with the flagellar antigens H25 and H28 revealed the genetic diversity of the fliC H25 and fliC H28 gene sequences in E. coli. Based on allele discrimination of these fliC-genes real-time PCR tests were designed for identification of EHEC O145:H25 and O145:H28. The fliC H25 genes present in O145:H25 were found to be very similar to those present in E. coli serogroups O2, O100, O165, O172 and O177 pointing to their common evolution but were different from fliC H25 genes of a multiple number of other E. coli serotypes. In a similar way, EHEC O145:H28 harbor a characteristic fliC H28 allele which, apart from EHEC O145:H28, was only found in enteropathogenic (EPEC) O28:H28 strains that shared some common traits with EHEC O145:H28. The real time PCR-assays targeting these fliC H25[O145] and fliC H28[O145] alleles allow better characterization of EHEC O145:H25 and EHEC O145:H28. Evaluation of these PCR assays in spiked ready-to eat salad samples resulted in specific detection of both types of EHEC O145 strains even when low spiking levels of 1–10 cfu/g were used. Furthermore these PCR assays allowed identification of non-motile E. coli strains which are serologically not typable for their H-antigens. The combined use of O-antigen genotyping (O145wzy) and detection of the respective fliC H25[O145] and fliC H28[O145] allele types contributes to improve identification and molecular serotyping of E. coli O145 isolates. PMID:26000885

Adaptação transcultural preliminar do Children's Eating Attitude Test (Cheat para o idioma português The preliminary transcultural adaptation of the Children's Eating Attitude Test (Cheat for portuguese

Directory of Open Access Journals (Sweden)

Mariana Loureiro Barillari

2011-12-01

Full Text Available OBJETIVOS: traduzir e adaptar o instrumento "Children's Eating Attitude Test" (Cheat para o idioma português, avaliar a equivalência semântica e a compreensão verbal. MÉTODOS: envolveu quatro etapas: (1 tradução, (2 retradução, (3 revisão técnica e avaliação semântica, (4 pré-teste - avaliação da compreensão verbal por especialistas em transtorno alimentar. RESULTADOS: as 26 questões foram traduzidas e adaptadas para o idioma português. A versão final constou dos mesmos 26 itens apresentando-se de forma clara e de fácil compreensão (valores médios superior a 4,5 - máximo 5,0. CONCLUSÕES: o instrumento encontra-se traduzido e adaptado para o idioma português demonstrando bons resultados no processo de adaptação transcultural. São necessárias análises de validade externa, equivalência de mensuração e reprodutibilidade.OBJECTIVES: to translate and adapt the "Children's Eating Attitude Test" (Cheat to Portuguese and evaluate the semantic equivalence and verbal comprehension. METHODS: the process involved four stages: (1 translation, (2 back-translation, (3 technical review and semantic evaluation, (4 pre-test - evaluation of verbal comprehension by specialists in eating disorders. RESULTS: the 26 questions were translated and adapted for Portuguese. The final version contained the same 26 items presented in a clear and easy-tounderstand format (average score higher than 4.5 - maximum 5.0. CONCLUSIONS: the tool has been translated and adapted for Portuguese and the transcultural adaptation process was a success. There is still a need for analyses of external validity, measurement equivalence and reproducibility.

Preparation and characterization of Bi26-2xMn2xMo10O69-d and Bi26.4Mn0.6Mo10-2yMe2yO69-d (Me = V, Fe Solid Solutions

Directory of Open Access Journals (Sweden)

Z. A. Mikhaylovskaya

2017-09-01

Full Text Available Single phase samples of bismuth molybdate, Bi26Mo10O69, doped with Mn on the bismuth sublattice and V, Fe on the molybdenum sublattice were found to crystallize in the triclinic Bi26Mo10O69 structure at low doping levels and in the monoclinic Bi26Mo10O69 structure - at higher dopant concentration. The assumption that all Mn ions have an oxidation state of +2 was confirmed by means of magnetic measurement results analysis using Curie-Weiss law. Conductivity was investigated using impedance spectroscopy. The conductivity of Bi26.4Mn0.6Mo9.6Fe0.4O69-d was 1.2*10-2 S*cm-1 at 973 K and 2.2*10-4 S*cm-1 at 623 K, and the conductivity of Bi26.4Mn0.6Mo9.2V0.8O69-d was 2.2*10-3 S*cm-1 at 973 K and 2.2*10-5 S*cm-1 at 623 K.

Evaluation of a multiplex real-time PCR method for detecting shiga toxin-producing Escherichia coli in beef and comparison to the U.S. Department of Agriculture Food Safety and Inspection Service Microbiology laboratory guidebook method.

Science.gov (United States)

Fratamico, Pina M; Wasilenko, Jamie L; Garman, Bradley; Demarco, Daniel R; Varkey, Stephen; Jensen, Mark; Rhoden, Kyle; Tice, George

2014-02-01

The "top-six" non-O157 Shiga toxin-producing Escherichia coli (STEC) serogroups (O26, O45, O103, O111, O121, and O145) most frequently associated with outbreaks and cases of foodborne illnesses have been declared as adulterants in beef by the U.S. Department of Agriculture Food Safety and Inspection Service (FSIS). Regulatory testing in beef began in June 2012. The purpose of this study was to evaluate the DuPont BAX System method for detecting these top six STEC strains and strains of E. coli O157:H7. For STEC, the BAX System real-time STEC suite was evaluated, including a screening assay for the stx and eae virulence genes and two panel assays to identify the target serogroups: panel 1 detects O26, O111, and O121, and panel 2 detects O45, O103, O145. For E. coli O157:H7, the BAX System real-time PCR assay for this specific serotype was used. Sensitivity of each assay for the PCR targets was ≥1.23 × 10(3) CFU/ml in pure culture. Each assay was 100% inclusive for the strains tested (20 to 50 per assay), and no cross-reactivity with closely related strains was observed in any of the assays. The performance of the BAX System methods was compared with that of the FSIS Microbiology Laboratory Guidebook (MLG) methods for detection of the top six STEC and E. coli O157:H7 strains in ground beef and beef trim. Generally, results of the BAX System method were similar to those of the MLG methods for detecting non-O157 STEC and E. coli O157:H7. Reducing or eliminating novobiocin in modified tryptic soy broth (mTSB) may improve the detection of STEC O111 strains; one beef trim sample inoculated with STEC O111 produced a negative result when enriched in mTSB with 8 mg/liter novobiocin but was positive when enriched in mTSB without novobiocin. The results of this study indicate the feasibility of deploying a panel of real-time PCR assay configurations for the detection and monitoring of the top six STEC and E. coli O157:H7 strains in beef. The approach could easily be adapted

Synthesis of ceramic powders of La{sub 9,56} (SiO{sub 4}){sub 6}O{sub 2,34} and La{sub 9,8}Si{sub 5,7}Mg{sub O,3}O{sub 26,}4 by modified sol-gel process; Sintese de pos ceramicos de La{sub 9,56} (SiO{sub 4}){sub 6}O{sub 2,34} e La{sub 9,8}Si{sub 5,7}Mg{sub O,3}O{sub 26,}4 por processo sol-gel modificado

Energy Technology Data Exchange (ETDEWEB)

Lira, Sabrina Lopes; Paiva, Mayara Rafaela Soares; Misso, Agatha Matos; Elias, Daniel Ricco; Yamagata, Chieko, E-mail: yamagata@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (CCTM/IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Centro de Ciencia e Tecnologia de Materiais

2012-07-01

Lanthanum silicate oxyapatite materials are promising for application as electrolyte in solid oxide fuel cells because of high ionic conductivity at temperatures between 600 deg C and 800 deg C. In this work, oxyapatites with the composition La{sub 9,56}(SiO{sub 4}){sub 6}O{sub 2,34}, and La{sub 9,8}Si{sub 5,7}Mg{sub 0,3}O{sub 26,4} were synthesized by using the sol-gel method, followed by precipitation. Initially, the gel of silica was synthesized from sodium silicate solution, by acid catalysis using lanthanum and magnesium chloride solution. Then, the La and Mg hydroxides were precipitated with NaOH in the gel. The powders were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and measurements of specific surface area. The crystalline oxyapatite phase of La{sub 9,56}(SiO{sub 4}){sub 6}O{sub 2,34}, and was La{sub 9,8}Si{sub 5,7}Mg{sub 0,3}O{sub 26,4} obtained by calcination at 900 deg C for 2 and 1h respectively (author)

Study of some direct reactions at 00 in 18O+26Mg and 18O+12C systems

International Nuclear Information System (INIS)

Payet, J.

1984-01-01

Direct transfer reactions induced by a 18 O beam on 12 C and 26 Mg targets, have been studied at the MP tandem (Orsay) with an experimental set up giving the possibility to have angular distributions up to 0 0 . Transfer reactions to discrete levels were analysed in the D.W.B.A. framework. This analysis confirms the interest as a spectroscopic tool of this 0 0 measurements. The ( 18 O, 16 O) and ( 18 O, 20 Ne) reactions were observed up to 40 MeV excitation energy. A diffractionnal model, including the one step direct transfer hypothesis, gives a qualitative agreement with the angular distributions and the energy spectra. A parametrization of a specific small angles development of the transfer amplitudes does not give good results with physical values for the parameters. 32 refs [fr

The absence of a flagellum leads to altered colony morphology, biofilm development and virulence in Vibrio cholerae O139

Science.gov (United States)

Watnick, Paula I.; Lauriano, Crystal M.; Klose, Karl E.; Croal, Laura; Kolter, Roberto

2010-01-01

Summary Throughout most of history, epidemic and pandemic cholera was caused by Vibrio cholerae of the serogroup O1. In 1992, however, a V. cholerae strain of the serogroup O139 emerged as a new agent of epidemic cholera. Interestingly, V. cholerae O139 forms biofilms on abiotic surfaces more rapidly than V. cholerae O1 biotype El Tor, perhaps because regulation of exopolysaccharide synthesis in V. cholerae O139 differs from that in O1 El Tor. Here, we show that all flagellar mutants of V. cholerae O139 have a rugose colony morphology that is dependent on the vps genes. This suggests that the absence of the flagellar structure constitutes a signal to increase exopolysaccharide synthesis. Furthermore, although exopolysaccharide production is required for the development of a three-dimensional biofilm, inappropriate exopolysaccharide production leads to inefficient colonization of the infant mouse intestinal epithelium by flagellar mutants. Thus, precise regulation of exopolysaccharide synthesis is an important factor in the survival of V. cholerae O139 in both aquatic environments and the mammalian intestine. PMID:11136445

A Emenda Constitucional 45 e a questão do acesso à justiça The Constitutional Amendment 45 and the acess to the justice

Directory of Open Access Journals (Sweden)

Ludmila Ribeiro

2008-12-01

Full Text Available A proposta deste artigo é discutir em que medida a Emenda Constitucional 45 pode (ou não alterar o cenário atual de acesso à justiça através da alteração de algumas normas relativas ao funcionamento do judiciário brasileiro. Este estudo se insere na seara denominada "direito e segurança jurídica" na medida em que pretende verificar de que forma alterações legais podem o escopo e o tipo de acesso que a população possui ao judiciário. Para tanto, além de uma revisão sobre o processo de constituição e reforma do sistema judiciário brasileiro, sobre os pressupostos teóricos da questão do acesso à justiça, este artigo revisou outras pesquisas nacionais sobre cada um dos temas escolhidos para esta abordagem no âmbito dessa emenda. As conclusões apontam para o fato de que várias das mudanças previstas pela EC/45 poderiam ser materializadas pelo simples cumprimento da legislação extraordinária já existente. Nesse sentido, a grande alteração instituída pela Emenda 45 foi a constituição do Conselho Nacional de Justiça como órgão destinado a avaliar o trabalho das cortes estaduais de justiça.This article analysis the 45th Amendment to the Brazilian Constitution, focusing on the possibilities that this rule has in enlarge the access to the justice in this country. This study is related to the area "right and legal security" because it is going to verify if this lawful alteration is able to improve the access that the people has to the Brazilian judiciary. Therefore, beyond a revision about the Constitution and reforms of the Brazilian Judicial System and the theoretical framework related to the access to the justice, this article overhauled other researches about the routine and organizational structure of the judiciary state courts. The results aim that, despite the 45th Constitutional Amendment intends to changes the judiciary system as a role; some of its disposition those could be done through the fulfillment of

Typing of O26 enterohaemorrhagic and enteropathogenic Escherichia coli isolated from humans and cattle with IS621 multiplex PCR-based fingerprinting.

Science.gov (United States)

Mainil, J G; Bardiau, M; Ooka, T; Ogura, Y; Murase, K; Etoh, Y; Ichihara, S; Horikawa, K; Buvens, G; Piérard, D; Itoh, T; Hayashi, T

2011-09-01

This study evaluated a typing method of O26:H11 enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) based on the variation in genomic location and copy numbers of IS621. Two multiplex PCRs, targeting either the left (5') or right (3') IS/chromosome junction of 12 IS621 insertion sites and one PCR specific of another truncated copy, were developed. Thirty-eight amplification profiles were observed amongst a collection of 69 human and bovine O26:H11 EHEC and EPEC. Seventy-one per cent of the 45 EHEC and EPEC with identical IS621 fingerprints within groups of two, three or four isolates had >85% pulsed field gel electrophoresis (PFGE) profile similarity, including four groups of epidemiologically related EHEC or EPEC, while most of the groups had identical IS621 fingerprints and PFGE profiles. The IS621 fingerprinting and the PFGE are complementary typing assays of EHEC and EPEC; though, the former is less discriminatory. The IS621 printing method represents a rapid (24 h) first-line surveillance and typing assay, to compare and trace back O26:H11 EHEC and EPEC during surveys in farms, multiple human cases and outbreaks. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology. No claim to Belgian Government works.

Classification of non-O157 shiga toxin-producing escherichia coli(STEC) serotypes with hyperspectral microscope imaging

Science.gov (United States)

Non-O157 Shiga toxin-producing Escherichia coli (STEC) strains such as O26, O45, O103, O111, O121 and O145 are recognized as serious outbreak to cause human illness due to their toxicity. A conventional microbiological method for cell counting is laborious and needs long time for the results. Since ...

Theoretical and experimental study of resonant 3d X-ray photoemission and resonant L3M45M45 Auger transition of PdO

International Nuclear Information System (INIS)

Uozumi, Takayuki; Kotani, Akio

2000-01-01

The observed 3d X-ray photoemission spectra (XPS), resonant 3dXPS (RXPS) at the L 3 edge and resonant L 3 M 45 M 45 Auger electron spectra (RAES) of 4d transition metal oxide PdO are successfully analyzed by means of an impurity Anderson model. The importance of Pd 4d-O 2p hybridization effect is especially emphasized in the interpretation of observed spectra. It causes charge transfer satellites in 3dXPS and L 3 M 45 M 45 RAES and mainly determines the structure of resonance enhancements in 3dRXPS. From the analysis of spectra, 4d-2p charge transfer energy Δ, 4d correlation energy U dd and 4d-2p transfer integral pdσ are estimated to be 5.5 eV, 4.5 eV and 2.1 eV, respectively. The character of the insulating energy gap PdO is also theoretically investigated: PdO is classified as an intermediate-type insulator due to the strong 4d-2p hybridization. (author)

Biocompatibility property of 100% strontium-substituted SiO2 -Al2 O3 -P2 O5 -CaO-CaF2 glass ceramics over 26 weeks implantation in rabbit model: Histology and micro-Computed Tomography analysis.

Science.gov (United States)

Basu, Bikramjit; Sabareeswaran, A; Shenoy, S J

2015-08-01

One of the desired properties for any new biomaterial composition is its long-term stability in a suitable animal model and such property cannot be appropriately assessed by performing short-term implantation studies. While hydroxyapatite (HA) or bioglass coated metallic biomaterials are being investigated for in vivo biocompatibility properties, such study is not extensively being pursued for bulk glass ceramics. In view of their inherent brittle nature, the implant stability as well as impact of long-term release of metallic ions on bone regeneration have been a major concern. In this perspective, the present article reports the results of the in vivo implantation experiments carried out using 100% strontium (Sr)-substituted glass ceramics with the nominal composition of 4.5 SiO2 -3Al2 O3 -1.5P2 O5 -3SrO-2SrF2 for 26 weeks in cylindrical bone defects in rabbit model. The combination of histological and micro-computed tomography analysis provided a qualitative and quantitative understanding of the bone regeneration around the glass ceramic implants in comparison to the highly bioactive HA bioglass implants (control). The sequential polychrome labeling of bone during in vivo osseointegration using three fluorochromes followed by fluorescence microscopy observation confirmed homogeneous bone formation around the test implants. The results of the present study unequivocally confirm the long-term implant stability as well as osteoconductive property of 100% Sr-substituted glass ceramics, which is comparable to that of a known bioactive implant, that is, HA-based bioglass. © 2014 Wiley Periodicals, Inc.

Meningococcal polysaccharide A O-acetylation levels do not impact the immunogenicity of the quadrivalent meningococcal tetanus toxoid conjugate vaccine: results from a randomized, controlled phase III study of healthy adults aged 18 to 25 years.

Science.gov (United States)

Lupisan, Socorro; Limkittikul, Kriengsak; Sosa, Nestor; Chanthavanich, Pornthep; Bianco, Véronique; Baine, Yaela; Van der Wielen, Marie; Miller, Jacqueline M

2013-10-01

In this study, we compared the immunogenicities of two lots of meningococcal ACWY-tetanus toxoid conjugate vaccine (MenACWY-TT) that differed in serogroup A polysaccharide (PS) O-acetylation levels and evaluated their immunogenicities and safety in comparison to a licensed ACWY polysaccharide vaccine (Men-PS). In this phase III, partially blinded, controlled study, 1,170 healthy subjects aged 18 to 25 years were randomized (1:1:1) to receive one dose of MenACWY-TT lot A (ACWY-A) (68% O-acetylation), MenACWY-TT lot B (ACWY-B) (92% O-acetylation), or Men-PS (82% O-acetylation). Immunogenicity was evaluated in terms of serum bactericidal activity using rabbit complement (i.e., rabbit serum bactericidal activity [rSBA]). Solicited symptoms, unsolicited adverse events (AEs), and serious AEs (SAEs) were recorded. The immunogenicities, in terms of rSBA geometric mean titers, were comparable for both lots of MenACWY-TT. The vaccine response rates across the serogroups were 79.1 to 97.0% in the two ACWY groups and 73.7 to 94.1% in the Men-PS group. All subjects achieved rSBA titers of ≥1:8 for all serogroups. All subjects in the two ACWY groups and 99.5 to 100% in the Men-PS group achieved rSBA titers of ≥1:128. Pain was the most common solicited local symptom and was reported more frequently in the ACWY group (53.9 to 54.7%) than in the Men-PS group (36.8%). The most common solicited general symptoms were fatigue and headache, which were reported by 28.6 to 30.3% and 26.9 to 31.0% of subjects, respectively. Two subjects reported SAEs; one SAE was considered to be related to vaccination (blighted ovum; ACWY-B group). The level of serogroup A PS O-acetylation did not affect vaccine immunogenicity. MenACWY-TT (lot A) was not inferior to Men-PS in terms of vaccine response and was well tolerated.

Bacteriemia por Vibrio cholerae no-O1, no-O139 en un paciente en hemodiálisis crónica Non-O1, non-O139 Vibrio cholerae bacteremia in a chronic hemodialysis patient

Directory of Open Access Journals (Sweden)

Mariela S. Zárate

2011-06-01

Full Text Available Vibrio cholerae no-O1, no-O139 es un agente poco frecuente como causal de bacteriemias y no hay informes que documenten su presencia en pacientes en hemodiálisis crónica. Se describe el caso de una paciente en hemodiálisis crónica que presentó un cuadro de sepsis, por lo cual inició un tratamiento con vancomicina y ceftacidima. Al cabo de seis horas y media de incubación en el sistema BACT/ALERT de hemocultivo, se evidenció la presencia de bacilos curvos gram negativos, posteriormente identificados como Vibrio cholerae mediante pruebas bioquímicas convencionales y el uso de los kits API 20 NE y VITEK 2. La evaluación del serogrupo y de la presencia de factores de patogenicidad, realizada en el laboratorio de referencia, determinó que el microorganismo hallado pertenecía al serogrupo no-O1, no-O139. No se detectó la toxina de cólera, tampoco el factor de colonización ni la toxina termoestable. El aislamiento presentó sensibilidad frente a ampicilina, trimetoprima-sulfametoxazol, ciprofloxacina, tetraciclina, ceftacidima y cefotaxima por el método de difusión con discos y por VITEK 2. La paciente cumplió 14 días de tratamiento con ceftacidima endovenosa, con evolución favorable.Non-O1, and non-O139 Vibrio cholerae is an infrequent cause of bacteremia. There are no reports of such bacteremia in chronic hemodialysis patients. This work describes the case of a chronic hemodialysis patient that had an episode of septicemia associated with dialysis. Blood cultures were obtained and treatment was begun with vancomycin and ceftazidime. After 6.5 hours of incubation in the Bact/Alert system there is evidence of gram-negative curved bacilli that were identified as Vibrio cholerae by conventional biochemical tests, API 20 NE and the VITEK 2 system. This microorganism was sent to the reference laboratory for evaluation of serogroup and virulence factors and was identified as belonging to the non-O1 and non-O139 serogroup. The cholera

Interface role in the enhanced photocatalytic activity of TiO2-Na0.9Mg0.45Ti3.55O8 nanoheterojunction

Directory of Open Access Journals (Sweden)

Ze-Qing Guo

2017-02-01

Full Text Available TiO2-Na0.9Mg0.45Ti3.55O8 (TiO2-NMTO nanocomposites were synthesized via a simple hydrothermal method. TiO2 nanoparticles were loaded on NMTO nanosheets with well matched lattices. The TiO2-NMTO nanoheterojunctions enjoyed high photodegradative ability for a RhB pollutant. The photoinduced electron-hole pairs were separated effectively by the TiO2-NMTO nanoheterojunctions, which were directly observed by surface potential measurements with a scanning Kelvin probe microscopy. The photogenerated electrons accumulate at interface due to the high density of interface states, and holes remain TiO2 and NMTO particles, other than they migrate from one part to another in heterojunctions by comparing the surface potentials under illumination with different wavelengths.

Synthesis and characterization of 64SiO2-26CaO-5P2O5-5CuO bioactive composition for the growth of hydroxyapatite layer by XRD, Raman and pH studies

Science.gov (United States)

Kaur, Pardeep; Singh, K. J.

2016-05-01

Bioactive sample with the nominal composition of 64SiO2-26CaO-5P2O5-5CuO has been prepared in the laboratory by using the sol-gel technique. The bioactivity of the prepared sample has been analyzed by using the Tris Simulated Body Fluid which has also been prepared in the laboratory. XRD and Raman techniques have been employedto probe the formation of hydroxyapatite layer. pH studies has also been undertaken to check the acidic/non-acidic behavior of sample. Growth of hydroxyapatite layer has been observed after one day on the surface of the sample. Moreover, sample has been observed to be non-acidic in nature.

Crystal structure of [bis(2,6-diisopropylphenyl phosphato-κO]tris(methanol-κOlithium methanol monosolvate

Directory of Open Access Journals (Sweden)

Mikhail E. Minyaev

2015-05-01

Full Text Available Crystals of the title compound, [Li{OOP(O-2,6-iPr2C6H32}(CH3OH3]·CH3OH or [Li(C24H34O4P(CH3OH3]·CH3OH, have been formed in the reaction between HOOP(O-2,6-iPr2C6H32 and LiOH in methanol. The title compound is of interest as it represents the first reported crystal structure of the family of lithium phosphate diesters. The {Li(CH3OH3[O2P(O-iPr2C6H32]} unit displays the Li atom in a slightly distorted tetrahedral coordination environment and exhibits one intramolecular O—H...O hydrogen bond between a coordinating methanol molecule and the terminal non-coordinating O atom of the phosphate group. The unit is connected with two non-coordinating methanol molecules through two intermolecular O—H...O hydrogen bonds, and with a neighbouring unit through two other O—H...O interactions. These intermolecular hydrogen bonds lead to the formation of infinite chains along [100]. There are no significant interactions between the chains.

Adaptação e validação de construto da Escala de Satisfação no Trabalho Adaptation and construct validation of Job Satisfaction Scale

Directory of Open Access Journals (Sweden)

Maria do Carmo Fernandes Martins

2006-12-01

Full Text Available O objetivo deste estudo foi revisar, validar e apurar a fidedignidade da Escala de Satisfação no Trabalho, composta por 116 itens agrupados em 14 fatores válidos com fidedignidade insatisfatória. A revisão semântica manteve 113 itens, aplicados a 600 trabalhadores-estudantes, sendo 51% mulheres, com nível de escolaridade média e superior, com idade média de 26 anos. Análises dos componentes principais e scree plot indicavam haver 7 a 12 componentes. A fatoração dos eixos principais com rotações Varimax e Promax reduziu a escala para 45 itens agrupados em seis fatores: "satisfação com suporte organizacional", "satisfação com utilidade social da organização e do trabalho", "satisfação com relacionamento afetivo no trabalho", "satisfação com reconhecimento profissional", "insatisfação com inadequação da chefia e falta de oportunidades" e "insatisfação com sobrecarga de trabalho" com alfas de 0,86, 0,81, 0,80, 0,82, 0,85 e 0,81. A estrutura do construto confirmou-se afetiva e multideterminada. Esta conclusão corroborou achados da área.The purpose of this study was revise semantic contents, validating and establishing the reliability of the Job Satisfaction Scale, formed by 116 items and grouped in 14 valid factors with unsatisfactory reliability. The semantic revision retained 113 applied to 600 workers-students, 51% women, at nocturnal schools of medium and superior scholarship with average age of 26 years old. Analyses of the principal components and scree plot pointed 7 to 12 components. The principal axis factoring (Varimax and Promax rotation reduced the scale to 45 items grouped in 6 factors: "satisfaction with organizational support", "satisfaction with social utility of the organization and of work", "satisfaction with affective relationship at work", "satisfaction with professional recognition", "insatisfaction with inadequacy of the working opportunities" and "insatisfaction with working overload". Cronbach

Misfit dislocations of anisotropic magnetoresistant Nd0.45Sr0.55MnO3 thin films grown on SrTiO3 (1 1 0) substrates

International Nuclear Information System (INIS)

Tang, Y.L.; Zhu, Y.L.; Meng, H.; Zhang, Y.Q.; Ma, X.L.

2012-01-01

Nd 0.45 Sr 0.55 MnO 3 is an A-type antiferromagnetic manganite showing obvious angular-dependent magnetoresistance, which can be tuned by misfit strain. The misfit strain relaxation of Nd 0.45 Sr 0.55 MnO 3 thin films is of both fundamental and technical importance. In this paper, microstructures of epitaxial Nd 0.45 Sr 0.55 MnO 3 thin films grown on SrTiO 3 (1 1 0) substrates by pulsed laser deposition were investigated by means of (scanning) transmission electron microscopy. The Nd 0.45 Sr 0.55 MnO 3 thin films exhibit a two-layered structure: a continuous perovskite layer epitaxial grown on the substrate followed by epitaxially grown columnar nanostructures. An approximately periodic array of misfit dislocations is found along the interface with line directions of both 〈1 1 1〉 and [0 0 1]. High-resolution (scanning) transmission electron microscopy reveals that all the misfit dislocations possess a〈1 1 0〉-type Burgers vectors. A formation mechanism based on gliding or climbing of the dislocations is proposed to elucidate this novel misfit dislocation configuration. These misfit dislocations have complex effects on the strain relaxation and microstructure of the films, and thus their influence needs further consideration for heteroepitaxial perovskite thin film systems, especially for films grown on substrates with low-symmetry surfaces such as SrTiO 3 (1 1 0) and (1 1 1), which are attracting attention for their potentially new functions.

Salmonella O48 Serum Resistance is Connected with the Elongation of the Lipopolysaccharide O-Antigen Containing Sialic Acid

Directory of Open Access Journals (Sweden)

Aleksandra Pawlak

2017-09-01

Full Text Available Complement is one of the most important parts of the innate immune system. Some bacteria can gain resistance against the bactericidal action of complement by decorating their outer cell surface with lipopolysaccharides (LPSs containing a very long O-antigen or with specific outer membrane proteins. Additionally, the presence of sialic acid in the LPS molecules can provide a level of protection for bacteria, likening them to human cells, a phenomenon known as molecular mimicry. Salmonella O48, which contains sialic acid in the O-antigen, is the major cause of reptile-associated salmonellosis, a worldwide public health problem. In this study, we tested the effect of prolonged exposure to human serum on strains from Salmonella serogroup O48, specifically on the O-antigen length. After multiple passages in serum, three out of four tested strains became resistant to serum action. The gas-liquid chromatography/tandem mass spectrometry analysis showed that, for most of the strains, the average length of the LPS O-antigen increased. Thus, we have discovered a link between the resistance of bacterial cells to serum and the elongation of the LPS O-antigen.

Caracterización de aislamientos de Vibrio cholerae no-O1, no-O139 asociados a cuadros de diarrea Characterization of Vibrio cholerae non-O1 and non-O139 isolates associated with diarrhea

Directory of Open Access Journals (Sweden)

S. González Fraga

2009-03-01

Full Text Available La infección por Vibrio cholerae, el agente causal del cólera, se trasmite al hombre por ingestión de agua y alimentos contaminados. Aunque son los serogrupos O1 y O139 los que habitualmente se asocian al cólera epidémico, los aislamientos de otros serogrupos también son causales de gastroenteritis e infecciones extra-intestinales. Durante el período 2003-2005, se investigó la presencia de V. cholerae en la materia fecal de niños con diarrea atendidos en el Hospital del Niño Jesús, Tucumán. Se recuperaron 34 aislamientos de V. cholerae no-O1, no-O139. Se determinaron sus perfiles de virulencia por PCR, la sensibilidad a los antimicrobianos y la diversidad genética por electroforesis en campo pulsado. Se obtuvieron ocho perfiles de virulencia, aunque ningún aislamiento fue positivo para la toxina colérica ni para la toxina termoestable. Cuatro aislamientos fueron positivos para el sistema de secreción de tipo tres. El 17,6% de los aislamientos fueron resistentes o de sensibilidad intermedia a ampicilina y el 5,9% fueron resistentes a trimetoprima-sulfametoxazol. Los aislamientos resultaron muy diversos: se hallaron 27 patrones distintos en 29 aislamientos tipificables por electroforesis en campo pulsado. A pesar de su baja incidencia, V. cholerae continúa siendo un agente causal de diarrea en niños, los que se ven afectados por una amplia variedad de cepas circulantes.Vibrio cholerae, etiologic agent of cholera, is transmitted to humans by ingestion of contaminated food or water. Even though serogroups O1 and O139 are the ones usually associated to epidemic cholera, isolates from other serogroups also cause gastroenteritis and extraintestinal infections. During the period 2003-2005, presence of V. cholerae in stools was investigated in children with diarrhea that seaked assistance at the Niño Jesús Hospital in Tucumán. Thirty four isolates of V. cholerae non-O1, non-O139 were recovered. We characterized the isolates studying

Characterization of free lytic bacteriophages isolated from compost against O145 Shiga toxin-producing Escherichia coli (STEC) as a potential biocontrol agent

Science.gov (United States)

Shiga toxin-producing E. coli (STEC), one of the most prevalent foodborne pathogens, are notorious for hemolytic uremic syndrome (HUS) and causing high mortality among children and the elder population after infection. Besides O157 STEC, non-O157 STEC—particularly serogroup O145—is commonly associat...

Composite (La0.45Nd0.25)Sr0.3MnO3/5CuO materials for magnetic refrigeration applications

Science.gov (United States)

El Maalam, K.; Balli, M.; Habouti, S.; Dietze, M.; Hamedoun, M.; Hlil, E.-K.; Es-Souni, M.; El Kenz, A.; Benyoussef, A.; Mounkachi, O.

2018-03-01

In this work, the magnetocaloric properties of (La0.45Nd0.25)Sr0.3MnO3 (LNSMO)-based composites are studied. The structural, microstructural, magnetic and magnetocaloric properties of LNSMO and LNSMO/5CuO samples were investigated aiming to particularly clarify the secondary phase (CuO) role in driving the magnetocaloric behavior. The main phase LNSMO crystallizes in a rhombohedral R-3C (1 6 7) configuration. The XRD patterns of composite samples show both perovskite LNSMO and monoclinic Tenorite CuO structures. The microstructural analysis unveils that the CuO phase is mainly present in the grain boundaries and segregates region. On the other hand, it was found that the magnetocaloric effect could be significantly enhanced by adding a small amount of CuO (5% weight ratio). For a magnetic field changing from 0 to 1.5 T, the corresponding isothermal entropy change was found to be 2.55 J/kg K for the LNSMO/5CuO composite while it is only about 1.1 J/kg K for the mother material LNSMO. Our finding should inspire and open new ways for the enhancement of the magnetocaloric effect in manganites-based materials.

Bioactivity of gel-glass powders in the CaO-SiO2 system: a comparison with ternary (CaO-P2O5-SiO2) and quaternary glasses (SiO2-CaO-P2O5-Na2O).

Science.gov (United States)

Saravanapavan, Priya; Jones, Julian R; Pryce, Russell S; Hench, Larry L

2003-07-01

Bioactive glasses react chemically with body fluids in a manner that is compatible with the repair processes of the tissues. This results in the formation of an interfacial bond between the glasses and living tissue. Bioactive glasses also stimulate bone-cell proliferation. This behavior is dependent on the chemical composition as well as the surface texture of the glasses. It has been recently reported that gel-derived monolith specimens in the binary SiO2 - CaO are bioactive over a similar molar range of SiO2 content as the previously studied ternary CaO-P2O5-SiO2 system. In this report, the preparation and bioactivity of the binary gel-glass powder with 70 mol % SiO2 is discussed and its bioactivity is compared with the melt-derived 45S5 (quaternary) Bioglass and sol-gel-derived 58S (ternary) bioactive gel-glass compositions. Dissolution kinetic parameters K(1) and K(2) were also computed based on the silicon release for all glass powders. It was shown that the simple two-component SiO2-CaO gel-glass powder is bioactive with comparable dissolution rates as the clinically used melt-derived 45S5 Bioglass powder and extensively studied sol-gel-derived 58S gel-glass powder. Copyright 2003 Wiley Periodicals, Inc.

Disfunção sexual: Avaliação de mulheres durante o terceiro trimestre gestacional

Directory of Open Access Journals (Sweden)

Ana Eliza Rios de Araújo Mathias

2015-08-01

Full Text Available Introdução: Disfunção sexual (DS é caracterizada pela falta, excesso, desconforto e/ou dor no desenvolvimento do ciclo da resposta sexual, se manifestando de forma persistente ou recorrente. Objetivo: Determinar a prevalência das disfunções sexuais femininas em gestantes de baixo risco no terceiro trimestre e verificar sua associação com fatores de risco. Métodos: É um estudo transversal composto por 102 gestantes de baixo risco durante o terceiro trimestre gestacional e com vida sexual ativa, que estiveram em acompanhamento nos postos de saúde das cidades de Petrolina (PE e Juazeiro (BA entre setembro de 2013 e março de 2014. Para isso, utilizou-se uma ficha de dados pessoais, com características sociodemográficas e clínicas e o questionário Female Sexual Function Index (FSFI. Foram calculadas prevalências e possíveis associações por meio do teste do χ2. Em todas as análises foi adotado o nível de significância de p˂0,05. Intervalos de confiança (IC95%, quando determinados, foram exatos. Resultados: Do total da amostra, 45,1% apresentaram disfunção sexual com taxa média de função sexual feminina de 26,15. Os menores escores de domínios foram em desejo e excitação. Ocorreu ainda associação de disfunção sexual com paridade. Conclusão: O presente estudo evidenciou uma taxa moderada de mulheres com disfunção sexual no terceiro trimestre gestacional, mostrando uma diminuição significativa em todos os domínios do FSFI. Além disso, constatou-se diferença estatisticamente significativa na associação de disfunção sexual com a variável paridade. Contudo, não foi encontrada diferença significativa na disfunção sexual com a variável idade, índice de massa corpórea, escolaridade e renda familiar.

Ilyukhinite (H3O,Na)14Ca6Mn2Zr3Si26O72(OH)2 • 3H2O, a New Mineral of the Eudialyte Group

Science.gov (United States)

Chukanov, N. V.; Rastsvetaeva, R. K.; Rozenberg, K. A.; Aksenov, S. M.; Pekov, I. V.; Belakovsky, D. I.; Kristiansen, R.; Van, K. V.

2017-12-01

A new eudialyte-group mineral, ilyukhinite, ideally (H3O,Na)14Ca6Mn2Zr3Si26O72(OH)2 · 3H2O, has been found in peralkaline pegmatite at Mt. Kukisvumchorr, Khibiny alkaline pluton, Kola Peninsula, Russia. It occurs as brownish orange, with vitreous luster anhedral grains up to 1 mm across in hydrothermally altered peralkaline rock, in association with aegirine, murmanite, albite, microcline, rhabdophane-(Ce), fluorite, sphalerite and molybdenite. The Mohs hardness is 5; cleavage is not observed. D meas 2.67(2), D calc 2.703 g/cm3. Ilyukhinite is optically uniaxial (-): ω = 1.585(2), ɛ = 1.584(2). The IR spectrum is given. The average chemical composition of ilyukhinite (wt %; electron microprobe, ranges given in parentheses; H2O determined by gas chromatography) is as follows: 3.07 (3.63-4.43) Na2O, 0.32 (0.28-0.52) K2O, 10.63 (10.26-10.90) CaO, 3.06 (2.74-3.22) MnO, 1.15 (0.93-1.37) FeO, 0.79 (0.51-0.89) La2O3, 1.21 (0.97-1.44) Ce2O3, 0.41 (0.30-0.56) Nd2O3, 0.90 (0.77-1.12) TiO2, 10.94 (10.15-11.21) ZrO2, 1.40 (0.76-1.68) Nb2O5, 51.24 (49.98-52.28) SiO2, 1.14 (0.89-1.37) SO3, 0.27 (0.19—0.38) Cl, 10.9(5 )H2O,-0.06-O = C1, total is 98.27. The empirical formula is H36.04(Na3.82K0.20)(Ca5.65Ce0.22La0.14Nd0.07)(Mn1.285Fe0.48)(Zr2.645Ti0.34)Nb0.31Si25.41S0.42Cl0.23O86.82. The crystal structure has been solved ( R = 0.046). Ilyukhinite is trigonal, R3 m; a = 14.1695(6) Å, b = 31.026(1) Å, V = 5394.7(7) Å3, Z = 3. The strongest XRD reflections [ d, Å (I, %) ( hkl)] are 11.44 (82) (101), 7.09 (70) (110), 6.02 (44) (021), 4.371 (89) 205), 3.805 (47) (303, 033), 3.376 (41) (131), 2.985 (100) (315, 128), 2.852 (92) (404). Ilyukhinite was named in memory of Vladimir V. Ilyukhin (1934-1982), an outstanding Soviet crystallographer. The type specimen of ilyukhinite has been deposited in the collection of the Natural History Museum, University of Oslo, Norway.

The Mobilome; A Major Contributor to Escherichia coli stx2-Positive O26:H11 Strains Intra-Serotype Diversity

Directory of Open Access Journals (Sweden)

Sabine Delannoy

2017-09-01

Full Text Available Shiga toxin-producing Escherichia coli of serotype O26:H11/H- constitute a diverse group of strains and several clones with distinct genetic characteristics have been identified and characterized. Whole genome sequencing was performed using Illumina and PacBio technologies on eight stx2-positive O26:H11 strains circulating in France. Comparative analyses of the whole genome of the stx2-positive O26:H11 strains indicate that several clones of EHEC O26:H11 are co-circulating in France. Phylogenetic analysis of the French strains together with stx2-positive and stx-negative E. coli O26:H11 genomes obtained from Genbank indicates the existence of four clonal complexes (SNP-CCs separated in two distinct lineages, one of which comprises the “new French clone” (SNP-CC1 that appears genetically closely related to stx-negative attaching and effacing E. coli (AEEC strains. Interestingly, the whole genome SNP (wgSNP phylogeny is summarized in the cas gene phylogeny, and a simple qPCR assay targeting the CRISPR array specific to SNP-CC1 (SP_O26-E can distinguish between the two main lineages. The PacBio sequencing allowed a detailed analysis of the mobile genetic elements (MGEs of the strains. Numerous MGEs were identified in each strain, including a large number of prophages and up to four large plasmids, representing overall 8.7–19.8% of the total genome size. Analysis of the prophage pool of the strains shows a considerable diversity with a complex history of recombination. Each clonal complex (SNP-CC is characterized by a unique set of plasmids and phages, including stx-prophages, suggesting evolution through separate acquisition events. Overall, the MGEs appear to play a major role in O26:H11 intra-serotype clonal diversification.

The Mobilome; A Major Contributor to Escherichia coli stx2-Positive O26:H11 Strains Intra-Serotype Diversity.

Science.gov (United States)

Delannoy, Sabine; Mariani-Kurkdjian, Patricia; Webb, Hattie E; Bonacorsi, Stephane; Fach, Patrick

2017-01-01

Shiga toxin-producing Escherichia coli of serotype O26:H11/H- constitute a diverse group of strains and several clones with distinct genetic characteristics have been identified and characterized. Whole genome sequencing was performed using Illumina and PacBio technologies on eight stx2 -positive O26:H11 strains circulating in France. Comparative analyses of the whole genome of the stx2 -positive O26:H11 strains indicate that several clones of EHEC O26:H11 are co-circulating in France. Phylogenetic analysis of the French strains together with stx2 -positive and stx -negative E. coli O26:H11 genomes obtained from Genbank indicates the existence of four clonal complexes (SNP-CCs) separated in two distinct lineages, one of which comprises the "new French clone" (SNP-CC1) that appears genetically closely related to stx -negative attaching and effacing E. coli (AEEC) strains. Interestingly, the whole genome SNP (wgSNP) phylogeny is summarized in the cas gene phylogeny, and a simple qPCR assay targeting the CRISPR array specific to SNP-CC1 (SP_O26-E) can distinguish between the two main lineages. The PacBio sequencing allowed a detailed analysis of the mobile genetic elements (MGEs) of the strains. Numerous MGEs were identified in each strain, including a large number of prophages and up to four large plasmids, representing overall 8.7-19.8% of the total genome size. Analysis of the prophage pool of the strains shows a considerable diversity with a complex history of recombination. Each clonal complex (SNP-CC) is characterized by a unique set of plasmids and phages, including stx -prophages, suggesting evolution through separate acquisition events. Overall, the MGEs appear to play a major role in O26:H11 intra-serotype clonal diversification.

Structure, spectra and thermal, mechanical, Faraday rotation properties of novel diamagnetic SeO2-PbO-Bi2O3-B2O3 glasses

Science.gov (United States)

Chen, Qiuling; Su, Kai; Li, Yantao; Zhao, Zhiwei

2018-06-01

Faraday rotation diamagnetic glass has attracted research attentions in photonics, sensing and magneto optical devices due to their high refractive index, wide transmittance in UV and Fourier transform infrared (FT-IR) range and temperature independent Faraday rotation. Selenite modified heavy metal oxides glasses with composition of xSeO2-(10-x) B2O3-45PbO-45Bi2O3 (x = 0, 1, 5 and 10mol%) and 15%SeO2-40%PbO-45%Bi2O3 have been fabricated by melt-quenching method in present study. The influence of SeO2 on glass forming ability, thermal, mechanical properties and Faraday rotation were evaluated through X-ray Diffraction (XRD), Fourier transforms infrared spectra (FT-IR), Raman, X-ray photoelectron spectroscopy (XPS), differential scanning calorimetry (DSC), Vicker's hardness and Verdet constant measurements. XRD spectra reveal that the good vitrification was achieved for glass with SeO2 amounts ≤10% even without B2O3. FT-IR, Raman and XPS spectra ascertain the existence of characteristic vibration of SeO4, SeO3, PbO4, BiO3 and BO3 units. The incorporation of SeO2 increases the connectivity of glassy network by increasing the Tg, thermal stability and mechanical hardness. The small band gap, high polarizable Se4+ ions and isolated SeO3 units contribute to Faraday rotation improvement.

Bis(2,6-dihydroxybenzoato-κ2O1,O1′(nitrato-κ2O,O′bis(1,10-phenanthroline-κ2N,N′cerium(III

Directory of Open Access Journals (Sweden)

Hongxiao Jin

2011-01-01

Full Text Available The mononuclear title complex, [Ce(C7H5O32(NO3(C12H8N22], is isostructural to other related lanthanide structures. The Ce atom is in a pseudo-bicapped square-antiprismatic geometry formed by four N atoms from two chelating 1,10-phenanthroline (phen ligands and by six O atoms, four from two 2,6-dihydroxybenzoate (DHB ligands and the other two from a nitrate anion. π–π stacking interactions between phen and DHB ligands [centroid–centroid distances = 3.513 (3 and 3.762 (2 Å] and phen and phen ligands [face-to-face separation = 3.423 (7 Å] of adjacent complexes stabilize the crystal structure. Intramolecular O—H...O hydrogen bonds are observed in the DHB ligands.

Isolation of 4,5-O-Dicaffeoylquinic Acid as a Pigmentation Inhibitor Occurring in Artemisia capillaris Thunberg and Its Validation In Vivo

Directory of Open Access Journals (Sweden)

Nadia Tabassum

2016-01-01

Full Text Available There is a continual need to develop novel and effective melanogenesis inhibitors for the prevention of hyperpigmentation disorders. The plant Artemisia capillaris Thunberg (Oriental Wormwood was screened for antipigmentation activity using murine cultured cells (B16-F10 malignant melanocytes. Activity-based fractionation using HPLC and NMR analyses identified the compound 4,5-O-dicaffeoylquinic acid as an active component in this plant. 4,5-O-Dicaffeoylquinic acid significantly reduced melanin synthesis and tyrosinase activity in a dose-dependent manner in the melanocytes. In addition, 4,5-O-dicaffeoylquinic acid treatment reduced the expression of tyrosinase-related protein-1. Significantly, we could validate the antipigmentation activity of this compound in vivo, using a zebrafish model. Moreover, 4,5-O-dicaffeoylquinic acid did not show toxicity in this animal model. Our discovery of 4,5-O-dicaffeoylquinic acid as an inhibitor of pigmentation that is active in vivo shows that this compound can be developed as an active component for formulations to treat pigmentation disorders.

Isolation of 4,5-O-Dicaffeoylquinic Acid as a Pigmentation Inhibitor Occurring in Artemisia capillaris Thunberg and Its Validation In Vivo.

Science.gov (United States)

Tabassum, Nadia; Lee, Ji-Hyung; Yim, Soon-Ho; Batkhuu, Galzad Javzan; Jung, Da-Woon; Williams, Darren R

2016-01-01

There is a continual need to develop novel and effective melanogenesis inhibitors for the prevention of hyperpigmentation disorders. The plant Artemisia capillaris Thunberg (Oriental Wormwood) was screened for antipigmentation activity using murine cultured cells (B16-F10 malignant melanocytes). Activity-based fractionation using HPLC and NMR analyses identified the compound 4,5-O-dicaffeoylquinic acid as an active component in this plant. 4,5-O-Dicaffeoylquinic acid significantly reduced melanin synthesis and tyrosinase activity in a dose-dependent manner in the melanocytes. In addition, 4,5-O-dicaffeoylquinic acid treatment reduced the expression of tyrosinase-related protein-1. Significantly, we could validate the antipigmentation activity of this compound in vivo, using a zebrafish model. Moreover, 4,5-O-dicaffeoylquinic acid did not show toxicity in this animal model. Our discovery of 4,5-O-dicaffeoylquinic acid as an inhibitor of pigmentation that is active in vivo shows that this compound can be developed as an active component for formulations to treat pigmentation disorders.

Mineralization of volatile organic compounds (VOCs) over the catalyst CuO-Co3O4-CeO2 and its applications in industrial odor control

KAUST Repository

Somekawa, Shouichi; Hagiwara, Toshiya; Fujii, Kyoko; Kojima, Masayuki; Shinoda, Tsutomu; Takanabe, Kazuhiro; Domen, Kazunari

2011-01-01

Volatile organic compounds (VOCs) present at ppm levels were decomposed over the catalyst CuO-Co3O4-CeO2 (Cu:Co:Ce = 10:45:45 in mol) in an attempt to scale up for industrial odor control. In addition to enhancing the catalytic activity, CuO-Co3O4 and CeO2 helped, respectively, to maintain the strength of the pelleted catalysts and inhibit their sintering. Using toluene as a VOC model compound, kinetic analysis of the total oxidation to carbon dioxide was conducted. The odor emitted from paint-drying processes could be eliminated effectively using CuO-Co3O4-CeO2 (Cu:Co:Ce = 10:45:45) pelleted catalysts (188 ml) in a large-scale system. © 2011 Elsevier B.V. All rights reserved.

Mineralization of volatile organic compounds (VOCs) over the catalyst CuO-Co3O4-CeO2 and its applications in industrial odor control

KAUST Repository

Somekawa, Shouichi

2011-12-01

Volatile organic compounds (VOCs) present at ppm levels were decomposed over the catalyst CuO-Co3O4-CeO2 (Cu:Co:Ce = 10:45:45 in mol) in an attempt to scale up for industrial odor control. In addition to enhancing the catalytic activity, CuO-Co3O4 and CeO2 helped, respectively, to maintain the strength of the pelleted catalysts and inhibit their sintering. Using toluene as a VOC model compound, kinetic analysis of the total oxidation to carbon dioxide was conducted. The odor emitted from paint-drying processes could be eliminated effectively using CuO-Co3O4-CeO2 (Cu:Co:Ce = 10:45:45) pelleted catalysts (188 ml) in a large-scale system. © 2011 Elsevier B.V. All rights reserved.

Framework 'interstitial' oxygen in La10(GeO4)5-(GeO5)O2 apatite electrolyte

International Nuclear Information System (INIS)

Pramana, S.S.; White, T.J.

2007-01-01

Oxygen conduction at low temperatures in apatites make these materials potentially useful as electrolytes in solid-oxide fuel cells, but our understanding of the defect structures enabling ion migration is incomplete. While conduction along [001] channels is dominant, considerable inter-tunnel mobility has been recognized. Using neutron powder diffraction of stoichiometric 'La 10 (GeO 4 ) 6 O 3 ', it has been shown that this compound is more correctly described as an La 10 (GeO 4 ) 5- (GeO 5 )O 2 apatite, in which high concentrations of interstitial oxygen reside within the channel walls. It is suggested that these framework interstitial O atoms provide a reservoir of ions that can migrate into the conducting channels of apatite, via a mechanism of inter-tunnel oxygen diffusion that transiently converts GeO 4 tetrahedra to GeO 5 distorted trigonal bipyramids. This structural modification is consistent with known crystal chemistry and may occur generally in oxide apatites. (orig.)

Detection of ctx gene positive non-O1/non-O139 V. cholerae in shrimp aquaculture environments.

Science.gov (United States)

Madhusudana, Rao B; Surendran, P K

2013-06-01

Water and post-larvae samples from black tiger (Penaeus monodon) shrimp hatcheries; pond water, pond sediment and shrimp from aquaculture farms were screened for the presence of V. cholerae. A V. cholerae-duplex PCR method was developed by utilizing V. cholerae species specific sodB primers and ctxAB genes specific primers. Incidence of V. cholerae was not observed in shrimp hatchery samples but was noticed in aquaculture samples. The incidence of V. cholerae was higher in pond water (7.6%) than in pond sediment (5.2%). Shrimp head (3.6%) portion had relatively higher incidence than shrimp muscle (1.6%). All the V. cholerae isolates (n = 42) belonged to non-O1/non-O139 serogroup, of which 7% of the V. cholerae isolates were potentially cholera-toxigenic (ctx positive). All the ctx positive V. cholerae (n = 3) were isolated from the pond water. Since, cholera toxin (CT) is the major contributing factor for cholera gravis, it is proposed that the mere presence of non-O1/non-O139 V. cholerae need not be the biohazard criterion in cultured black tiger shrimp but only the presence of ctx carrying non-O1/non-O139 V. cholerae may be considered as potential public health risk.

Anomalias branquiais: revisão de 23 casos

Directory of Open Access Journals (Sweden)

Rogério Aparecido Dedivitis

Full Text Available A anomalia branquial é a doença congênita mais comum vista pelo cirurgião de cabeça e pescoço. Vinte e três pacientes portadores de anomalias branquiais tratados no período de 1994 a 1997 foram revisados. O diagnóstico clínico foi documentado por ultra-sonografia e comprovado pelo exame histopatológico. Encontramos seis anomalias de primeiro arco (26%; 16 do segundo arco (69,5%; e uma do terceiro (4,5%. Não houve anomalia do quarto arco. Após um seguimento que variou de três a 43 meses, não houve caso de recidiva. A prevalência de anomalias do segundo arco é compatível com a literatura, porém, foi alta a incidência do primeiro arco. O emprego da técnica cirúrgica adequada, com a ressecção de todo o trajeto fistuloso, mostrou-se eficaz.

Crescimento do feijão caupi submetido a inoculação com rizóbio e irrigação com água salina

Directory of Open Access Journals (Sweden)

Jailma Ribeiro de Andrade

2012-10-01

Full Text Available A fixação biológica do nitrogênio (FBN é caracterizada pela conversão do nitrogênio gasoso (N2 em nitrogênio amoniacal (NH4, forma disponível às plantas. A cultura do feijão caupi gera em média 1.055.334 empregos, o potencial de suprimento alimentar é de 26.486.755 pessoas e o valor da produção é de R$ 643,09 milhões. Objetivou-se com esse trabalho avaliar parâmetros de crescimento no Feijão caupi irrigado com água salina. O experimento foi conduzido em casa de vegetação da Universidade Federal de Campina Grande (UFCG. Os tratamentos foram constituídos de diferentes níveis de salinidade da água de irrigação (1,5; 3,0; 4,5 e 6,0 dS m-1, sendo cada unidade experimental representada por uma planta/vaso, com capacidade de 1,5 Kg de solo. Os resultados obtidos foram submetidos às análises de variância, pelo teste de Tukey a 1 e 5% de significância. O acréscimo da salinidade afeta o número de folhas, diâmetro do caule e área foliar. Quanto ao desenvolvimento da cultura, todas as variáveis são afetadas após os 30 DAE.

Propane-1,2-diammonium bis(pyridine-2,6-dicarboxylato-κ3O,N,O′nickelate(II tetrahydrate

Directory of Open Access Journals (Sweden)

Mohammad Ghadermazi

2008-07-01

Full Text Available The reaction of nickel(II nitrate hexahydrate, propane-1,2-diamine and pyridine-2,6-dicarboxylic acid in a 1:2:2 molar ratio in aqueous solution resulted in the formation of the title compound, (C3H12N2[Ni(C7H3NO42]·4H2O or (p-1,2-daH2[Ni(pydc2]·4H2O (where p-1,2-da is propane-1,2-diamine and pydcH2 is pyridine-2,6-dicarboxylic acid. The geometry of the resulting NiN2O4 coordination can be described as distorted octahedral. Considerable C=O...π stacking interactions are observed between the carboxylate C=O groups and the pyridine rings of the (pydc2− fragments, with O...π distances of 3.1563 (12 and 3.2523 (12 Å and C=O...π angles of 95.14 (8 and 94.64 (8°. In the crystal structure, a wide range of non-covalent interactions, consisting of hydrogen bonding [O—H...O, N—H...O and C—H...O, with D...A distances ranging from 2.712 (2 to 3.484 (2 Å], ion pairing, π–π [centroid-to-centroid distance = 3.4825 (8 Å] and C=O...π stacking, connect the various components to form a supramolecular structure.

Revisão de 45 casos de tuberculose miliar em adultos - estudos clínicopatológicos

Directory of Open Access Journals (Sweden)

Adrelirio José Rios Gonçalves

1972-04-01

Full Text Available Os autores apresentem uma revisão de 45 casos de tuberculose viiliar em adultos, sendo que 42 foram selecionados de urn total de 4.000 necropsias. Uma correlação clínico-patológica é feita senão nitidamente evidenciado c baixo grau de suspeita clinica da doenca e a relativa freqüência desta infecção em Hospitais Gerais conseqüente à mimetização com outras patologias. Uma classificação histovatológica da condição é feita, assim como os principais órgãos atingidos são assinalados. Discussão a respeito dos mecanismos patogênicos é tentada. Finalizam com uma revisão a respeito dos problemas gerais envolvidos na tuberculose miliar.

Subsolidus phase relations of Bi2O3-Nd2O3-CuO

International Nuclear Information System (INIS)

Sun Yezhou

1997-01-01

The subsolidus phase relations of the Bi 2 O 3 -Nd 2 O 3 -CuO ternary system and its binary systems along with crystallographic parameters of the compounds were investigated by X-ray powder diffraction and differential thermal analysis. The room temperature section of the phase diagram of the Bi 2 O 3 -Nd 2 O 3 -CuO system can be divided into two diphase regions and six triphase regions. No ternary compound was found. There exist two solid solutions (α, β) and a compound Bi 0.55 Nd 0.45 O 1.5 in the (Bi 2 O 2 ) 1-x (Nd 2 O 3 ) x system. Both solid solution α (0.05≤x≤0.30) and β (0.53≤x≤0.73) belong to the rhombohedral system (R3m). The lattice parameters represented by a hexagonal cell are a=3.9832(4), c=27.536(5) A for Bi 0.8 Nd 0.2 O 1.5 (α phase) and a=3.8826(3), c=9.727(1) A for Bi 0.4 Nd 0.8 O 1.5 (β phase). The Bi 0.55 Nd 0.45 O 1.5 compound crystallizes in a face-centered cubic (f.c.c.) lattice with a=5.5480(2) A. (orig.)

Improved traceability of Shiga-toxin-producing Escherichia coli using CRISPRs for detection and typing.

Science.gov (United States)

Delannoy, Sabine; Beutin, Lothar; Fach, Patrick

2016-05-01

Among strains of Shiga-toxin-producing Escherichia coli (STEC), seven serogroups (O26, O45, O103, O111, O121, O145, and O157) are frequently associated with severe clinical illness in humans. The development of methods for their reliable detection from complex samples such as food has been challenging thus far, and is currently based on the PCR detection of the major virulence genes stx1, stx2, and eae, and O-serogroup-specific genes. However, this approach lacks resolution. Moreover, new STEC serotypes are continuously emerging worldwide. For example, in May 2011, strains belonging to the hitherto rarely detected STEC serotype O104:H4 were identified as causative agents of one of the world's largest outbreak of disease with a high incidence of hemorrhagic colitis and hemolytic uremic syndrome in the infected patients. Discriminant typing of pathogens is crucial for epidemiological surveillance and investigations of outbreaks, and especially for tracking and tracing in case of accidental and deliberate contamination of food and water samples. Clustered regularly interspaced short palindromic repeats (CRISPRs) are composed of short, highly conserved DNA repeats separated by unique sequences of similar length. This distinctive sequence signature of CRISPRs can be used for strain typing in several bacterial species including STEC. This review discusses how CRISPRs have recently been used for STEC identification and typing.

Survival of Antibiotic Resistant and Antibiotic Sensitive Strains of E. coli O157 and E. coli O26 in Food Matrices.

OpenAIRE

Walsh, Ciara; Duffy, Geraldine; Blair, I. S.; McDowell, D. A.

2006-01-01

Escherichia coli O157:H7 or E. coli O26, which were AS (antibiotic sensitive), AR (laboratory created antibiotic resistant mutants), or naturally MAR (multi-antibiotic resistant), were inoculated into laboratory media, yoghurt or orange juice and their growth/survival monitored during enrichment at 37 °C or storage at 4 °C. The strains were also inoculated into minced beef and their thermal inactivation (D-values) examined at 55 °C, with and without a prior heat shock at 48 °C. The growth kin...

ZnO-PbO-B2O3 glasses as gamma-ray shielding materials

DEFF Research Database (Denmark)

Singh, H.; Singh, K.; Gerward, Leif

2003-01-01

Values of the gamma-ray mass-attenuation coefficient, the photon mean free path (MFP), the effective atomic number and the effective electron density have been determined experimentally for xZnO.2xPbO.(1-3x)B2O3 (x = 0.1-0.26) glasses at photon energies 511, 662, 1173 and 1332 keV and compared wi...... with theoretical data. The specific volume of the glasses has been derived from density measurements and studied as a function of composition. It is pointed out that these glasses have potential applications in radiation shielding.......Values of the gamma-ray mass-attenuation coefficient, the photon mean free path (MFP), the effective atomic number and the effective electron density have been determined experimentally for xZnO.2xPbO.(1-3x)B2O3 (x = 0.1-0.26) glasses at photon energies 511, 662, 1173 and 1332 keV and compared...

The hybrid nanostructure of MnCo2O4.5 nanoneedle/carbon aerogel for symmetric supercapacitors with high energy density

Science.gov (United States)

Hao, Pin; Zhao, Zhenhuan; Li, Liyi; Tuan, Chia-Chi; Li, Haidong; Sang, Yuanhua; Jiang, Huaidong; Wong, C. P.; Liu, Hong

2015-08-01

Current applications of carbon-based supercapacitors are limited by their low energy density. One promising strategy to enhance the energy density is to couple metal oxides with carbon materials. In this study, a porous MnCo2O4.5 nanoneedle/carbon aerogel hybrid nanostructure was synthesized by assembling MnCo2O4.5 nanoneedle arrays on the surface of channel walls of hierarchical porous carbon aerogels derived from chitosan for the supercapacitor application. The synthetic process of the hybrid nanostructure involves two steps, i.e. the growth of Mn-Co precursors on carbon aerogel by a hydrothermal process and the conversion of the precursor into MnCo2O4.5 nanoneedles by calcination. The carbon aerogel exhibits a high electrical conductivity, high specific surface area and porous structure, ensuring high electrochemical performance of the hybrid nanostructure when coupled with the porous MnCo2O4.5 nanoneedles. The symmetric supercapacitor using the MnCo2O4.5 nanoneedle/carbon aerogel hybrid nanostructure as the active electrode material exhibits a high energy density of about 84.3 Wh kg-1 at a power density of 600 W kg-1. The voltage window is as high as 1.5 V in neutral aqueous electrolytes. Due to the unique nanostructure of the electrodes, the capacitance retention reaches 86% over 5000 cycles.Current applications of carbon-based supercapacitors are limited by their low energy density. One promising strategy to enhance the energy density is to couple metal oxides with carbon materials. In this study, a porous MnCo2O4.5 nanoneedle/carbon aerogel hybrid nanostructure was synthesized by assembling MnCo2O4.5 nanoneedle arrays on the surface of channel walls of hierarchical porous carbon aerogels derived from chitosan for the supercapacitor application. The synthetic process of the hybrid nanostructure involves two steps, i.e. the growth of Mn-Co precursors on carbon aerogel by a hydrothermal process and the conversion of the precursor into MnCo2O4.5 nanoneedles by

Caracterização e relacionamento antigênico de três novos Bunyavirus no grupo Anopheles A (Bunyaviridae dos arbovirus Characterization and antigenic relationship of three new Bunyavirus in the Anopheles A serogroup (Bunyaviridae of arboviruses

Directory of Open Access Journals (Sweden)

Jorge Fernando Soares Travassos da Rosa

1992-06-01

Full Text Available São descritos o isolamento e a caracterização de três novos arbovirus isolados na região da Usina Hidro-Elétrica de Tucuruí (UHE-TUC. Os três novos arbovirus pertencem ao grupo Anopheles A(ANA, gênero Bunyavirus (família Bunyaviridae. Os vírus Tucuruí (TUC, Caraipé (CPE e Arumateua (ART são relacionados entre si e com o vírus Trombetas (TBT, formando dentro do grupo ANA um complexo chamado Trombetas. Os arbovirus TUC, CPE e ART foram obtidos a partir de lotes de mosquitos Anopheles (Nyssorhynchus sp capturados em Tucuruí, nas proximidades da usina hidrelétrica de Tucuruí, Estado do Pará, nos meses de fevereiro, agosto e outubro de 1984, respectivamente. Até o final de 1990 os vírus TUC, CPE e ART foram isolados 12, 32 e 28 vezes respectivamente, sempre na região da UHE-TUC, exceção feita ao vírus TUC, do qual se obteve uma amostra procedente de Balbina, onde também foi construída uma hidroelétrica. Até o presente, esses vírus só foram isolados a partir de mosquitos do grupo An. (Nys. principalmente, a partir das espécies An. (Nys. nuneztovari e An. (Nys. triannulatus também consideradas vetores secundários da malária na Amazônia Brasileira. Testes sorológicos executados com soros humanos e de diversas espécies de animais silvestres foram negativos, com exceção de um soro de um carnívoro de espécie Nasua nasua que neutralizou a amostra TUC em títulos de 2.6 índice logaritmico de neutralização (ILN.The isolation and characterization of three new viruses obtained from the Tucuruí hydroelectric dam region is repeated. These three agents belong to the Anopheles A serogroup, genus Bunyavirus, Bunyaviridae. The Tucuruí (TUC, Caraipe (CPE and Arumateua (ART viruses have close relationships with each other and with Trombetas (TBT virus, an Anopheles A virus previously isolated in the Amazon Region of Brazil. These viruses form the "Trombetas complex". TUC, CPE and ART viruses were obtained from pools of

Avaliação do padrão coliformes a 45ºC e comparação da eficiência das técnicas dos tubos múltiplos e Petrifilm EC na detecção de coliformes totais e Escherichia coli em alimentos Coliforms at 45ºc and comparison of most probable number method and Petrifilm EC for enumeration of total coliforms and Escherichia coli of foods

Directory of Open Access Journals (Sweden)

M.P. Silva

2006-06-01

Full Text Available Coliformes fecais são definidos como coliformes capazes de fermentar a lactose com produção de gás em 48 h a 45ºC. Escherichia coli, juntamente com algumas cepas de Enterobacter e Klebsiella, podem apresentar essas características. Entretanto, apenas a presença de Escherichia coli em alimentos indica contaminação fecal por ser encontrada em grande quantidade no trato gastrointestinal do homem e animais de sangue quente, não sendo isolada normalmente em outros nichos. A denominação clássica de coliformes fecais foi alterada para coliformes a 45ºC, na Resolução nº 12 da Agência Nacional de Vigilância Sanitária. O objetivo deste trabalho foi avaliar a presença de E. coli entre os coliformes a 45ºC e comparar a eficiência das técnicas dos tubos múltiplos e Petrifilm EC na detecção de coliformes totais e E. coli em queijo Minas, lingüiça frescal, hortaliças e fubá. Petrifilm EC mostrou-se mais sensível na detecção de E. coli em relação ao método de tubos múltiplos, o qual apresentou resultados falso-negativos ou contagens subestimadas de E. coli, principalmente para amostras de alimentos de origem animal. Petrifilm EC foi o mais eficiente e prático, sendo um método alternativo adequado para a enumeração de coliformes totais e E. coli em alimentos.Fecal coliforms are defined as coliforms that ferment lactose with gas production within 48 hours at 45ºC. Escherichia coli and some strains of Enterobacter and Klebsiella show these same characteristics. However, only the presence of E. coli can indicate fecal contamination of foods, because it is abundantly found in the intestinal tract of all warm-blooded animals, including the humans, and it is not usually found in others niches. The classic denomination of fecal coliforms was changed to coliforms at 45ºC in the Resolution nº 12/2001 of the National Surveillance Agency of Brazil. The objectives of this study were to analyse the presence of E. coli among the

TX 2000: total reflection and 45o energy dispersive x-ray fluorescence spectrometer

International Nuclear Information System (INIS)

Pasti, F.; Torboli, A.; Valdes, M.

2000-01-01

This equipment, developed by Ital Structures, combines two kinds of energy dispersive X-ray fluorescence techniques, the first using total reflection geometry and the second conventional 45 o geometry. The equipment is completely controlled by a PC and to reach the condition of total reflection is very easy because it is enough to load the file with the right position for the corresponding energy. In this apparatus we used an x-ray tube with an alloy anode of Mo/W with a long fine focus at 2200 W. To monochromatize the x-ray beam while choosing, for example, the Mo K alpha or W L alpha or a piece of white spectrum of 33 keV, we use a highly reflective multilayer made of Si/W with 2d = 45.5 A o . The detector used in the equipment is a lithium drifted silicon detector (Si(Li)) with an excellent energy resolution of 135 eV at 5.9 keV and 1000 cps. We developed two programs written in Windows 95, 98 and NT for a 32 bit microprocessor. The first one is called TYACQ32 and has the following functions: first, complete control of the hardware, second automatic alignment of the TX 2000 spectrometer and third acquisition of spectra. The second program is EDXRF32. This is a program to accomplish spectrum and quantitative analysis for TXRF and EDXRF 45 o degrees analysis. (author)

Redetermination of the cubic struvite analogue Cs[Mg(OH26](AsO4

Directory of Open Access Journals (Sweden)

Matthias Weil

2009-01-01

Full Text Available In contrast to the previous refinement from photographic data [Ferrari et al. (1955. Gazz. Chim. Ital. 84, 169–174], the present redetermination of the title compound, caesium hexaaquamagnesium arsenate(V, revealed the Cs atom to be on Wyckoff position 4d instead of Wyckoff position 4b of space group Foverline{4}3m. The structure can be derived from the halite structure. The centres of the complex [Mg(OH26] octahedra and the AsO4 tetrahedra (both with overline{4}3m symmetry are on the respective Na and Cl positions. The building units are connected to each other by O—H...O hydrogen bonds. The Cs+ cations (overline{4}3m symmetry are located in the voids of this arrangement and exhibit a regular cuboctahedral 12-coordination to the O atoms of the water molecules. The O atom bonded to As has 2mm site symmetry (Wyckoff position 24f and the water-molecule O atom has m site symmetry (Wyckoff position 48h.

ESTUDO DA COBERTURA VEGETAL/USO DA TERRA NOS ANOS DE 1987 E 2007 NO NÚCLEO DE DEGRADAÇÃO/DESERTIFICAÇÃO DE SÃO RAIMUNDO NONATO - PIAUÍ

Directory of Open Access Journals (Sweden)

Cláudia Maria Sabóia de AQUINO

2012-07-01

Full Text Available A cobertura vegetal é um componente de grande relevância para o equilíbrio ambiental, uma vez que assegura a proteção dos solos contra a erosão hídrica. Esta é recomendada pela ONU como um indicador para fins de avaliação do processo de desertificação. Considerando que aproximadamente 45,3% do estado do Piauí apresentam suscetibilidade a desertificação, este trabalho objetivou analisar de forma espaço-temporal, com o emprego de técnicas de sensoriamento remoto (detecção de mudanças, a dinâmica da cobertura vegetal/uso da terra no Núcleo de São Raimundo Nonato, para fins de avaliar a degradação/desertificação. Os resultados mostraram que houve decréscimo significativo da classe de cobertura vegetal/uso da terra do tipo agricultura+solos exposto. Esta decresceu de 31,1% em 1987 para 26,8% em 2007. Já na classe caatinga arbustiva densa, constatou-se incremento de 24,9% para 39,3%. Os resultados sugerem o aumento da proteção oferecida pela cobertura vegetal que em 1987 era de 15,7% passou para 17,5% em 2007, com consequente diminuição do processo de degradação/desertificação na área de estudo.

Pathogenicity of Vibrio anguillarum serogroup O1 strains compared to plasmids, outer membrane protein profiles and siderophore production

DEFF Research Database (Denmark)

Pedersen, K.; Gram, Lone; Austin, D.A.

1997-01-01

The virulence of 18 strains of Vibrio anguillarum serogroup 01 was compared to plasmid content, expression of siderophores and outer membrane proteins. All strains, irrespective of plasmid content, produced siderophores and inducible outer membrane proteins under iron-limited conditions. Only str...

Hydrothermal synthesis and crystal structure of a new molybdenum oxide compound with manganese-o-phen subunit: [Mn(o-phen)(H2O)MoO4]·H2O (o-phen=o-phenanthroline)

International Nuclear Information System (INIS)

Zhang Quanzheng; Lu Canzhong; Yang Wenbin; Chen Shumei; Yu Yaqin; He Xiang; Yan Ying; Liu Jiuhui; Xu Xinjiang; Xia Changkun; Wu Xiaoyuan; Chen Lijuan

2004-01-01

A new one-dimensional molybdenum oxide compound with manganese-o-phen subunit: [Mn(o-phen)(H 2 O)MoO 4 ]·H 2 O (1) (o-phen=o-phenanthroline) was synthesized by the hydrothermal reaction of Na 2 MoO 4 ·2H 2 O, MnSO 4 ·H 2 O, oxalic acid, o-phenanthroline (o-phen) and water. Its structure was determined by elemental analyses, ESR spectrum, TG analysis, IR spectrum and single-crystal X-ray diffraction. Compound 1 crystallizes in triclinic system, space group P-1 with a=7.0401(2) A, b=10.4498(2) A, c=10.5720(2) A, α=73.26(7) deg., β=83.34(8) deg., γ=77.33(9) deg., V=725.5089(0) A 3 , Z=2, and R 1 =0.0322 for 2337 observed reflections. Compound 1 exhibits one-dimensional chain structure. The chains are linked up via hydrogen bonding to 2D layers, which are further assembled through π-π stacking interactions to a 3D supermolecular structure

Altas habilidades/superdotação: mitos e dilemas docentes na indicação para o atendimento

Directory of Open Access Journals (Sweden)

Sonia Maria Lourenço de Azevedo

Full Text Available O baixo quantitativo de indicação de alunos com altas habilidades/superdotação apontado pelos professores de um Município da periferia do Rio de Janeiro motivou a investigação das possíveis dificuldades encontradas pelos professores nesse processo. Os sujeitos desta pesquisa são 52 professores divididos em dois grupos: 26 professores que indicaram alunos para o programa de alunos com altas habilidades/ superdotação da Secretaria Municipal de Educação desse Município (grupo 1, e 26 professores que não indicaram alunos (grupo 2. Os fatores utilizados nesta pesquisa foram: (a perfil sociodemográfico dos respondentes; (b levantamento de possíveis elementos da representação social desse professor por meio dos termos indutores: altas habilidades/superdotação e educação especial; (c três dilemas baseados na teoria de julgamento moral e nos mitos referentes a altas habilidades/superdotação. conclui-se que, em ambos os grupos, há fortes indícios de que os mitos acerca das altas habilidades/superdotação influenciam a indicação/não indicação desses alunos, e que esses mitos devem ser discutidos junto aos professores de modo a facilitar a indicação

Poly[bis(μ-2,6-dimethylpyridinium-3,5-dicarboxylato-κ2O3:O5copper(II

Directory of Open Access Journals (Sweden)

Guang-Feng Hou

2008-12-01

Full Text Available In the title coordination polymer, [Cu(C9H8NO42]n, the Cu atom, located on a twofold rotation axis, is four coordinate in a distorted square-planar environment. Each 2,6-dimethylpyridinium-3,5-dicarboxylate anion bridges two Cu atoms, forming a two-dimensional coordination polymer. A three-dimensional supramolecular network is built from N—H...O hydrogen bonds involving the pyridinium NH and the carboxyl COO groups.

Structural and magnetic properties of SiO{sub 2}-CaO-Na{sub 2}O-P{sub 2}O{sub 5} containing BaO-Fe{sub 2}O{sub 3} glass-ceramics

Energy Technology Data Exchange (ETDEWEB)

Leenakul, W.; Kantha, P.; Pisitpipathsin, N. [Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200 (Thailand); Rujijanagul, G.; Eitssayeam, S. [Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200 (Thailand); Materials Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai 50200 (Thailand); Pengpat, K., E-mail: kamonpan.p@cmu.ac.th [Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200 (Thailand); Materials Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai 50200 (Thailand)

2013-01-15

The incorporation method was employed to produce bioactive glass-ceramics from the BaFe{sub 12}O{sub 19}-SiO{sub 2}-CaO-Na{sub 2}O-P{sub 2}O{sub 5} glass system. The ferrimagnetic BaFe{sub 12}O{sub 19} was first prepared using a simple mixed oxide method, where the oxide precursors of 45S5 bioglass were initially mixed and then melted to form glass. The devitrification of Na{sub 3}Ca{sub 6}(PO{sub 4}){sub 5} and Fe{sub 3}O{sub 4} was observed in all of the quenched glass samples. The glass samples were then subjected to a heat treatment schedule for further crystallization. It was found that the small traces of BaFe{sub 12}O{sub 19} phases started to crystallize in high BF content samples of 20 and 40 wt%. These samples also exhibited good magnetic properties comparable to that of other magnetic glass-ceramics. The bioactivity of the BF glass-ceramics improved with increasing BF content as was evident by the formation of bone-like apatite layers on the surface of all of the glass-ceramics after soaking in SBF for 14 days. The results support the use of these bioactive glass-ceramics for hyperthermia treatment within the human body. - Highlights: Black-Right-Pointing-Pointer BF addition improves the magnetic property and bioactivity of 45S5 bioglasses. Black-Right-Pointing-Pointer Bioglass-ceramics exhibited soft magnetic properties with Mr=14.850 emu/g. Black-Right-Pointing-Pointer Magnetic property can be enhanced by crystallization of BF in 45S5 bioglasses.

Selective phenol methylation to 2,6-dimethylphenol in a fluidized bed of iron-chromium mixed oxide catalyst with o-cresol circulation.

Science.gov (United States)

Zukowski, Witold; Berkowicz, Gabriela; Baron, Jerzy; Kandefer, Stanisław; Jamanek, Dariusz; Szarlik, Stefan; Wielgosz, Zbigniew; Zielecka, Maria

2014-01-01

2,6-dimethylphenol (2,6-DMP) is a product of phenol methylation, especially important for the plastics industry. The process of phenol methylation in the gas phase is strongly exothermic. In order to ensure good temperature equalization in the catalyst bed, the process was carried out using a catalyst in the form of a fluidized bed - in particular, the commercial iron-chromium catalyst TZC-3/1. Synthesis of 2,6-dimethylphenol from phenol and methanol in fluidized bed of iron-chromium catalyst was carried out and the fluidization of the catalyst was examined. Stable state of fluidized bed of iron-chromium catalyst was achieved. The measured velocities allowed to determine the minimum flow of reactants, ensuring introduction of the catalyst bed in the reactor into the state of fluidization. Due to a high content of o-cresol in products of 2,6-dimethylphenol synthesis, circulation in the technological node was proposed. A series of syntheses with variable amount of o-cresol in the feedstock allowed to determine the parameters of stationary states. A stable work of technological node with o-cresol circulation is possible in the temperature range of350-380°C, and o-cresolin/phenolin molar ratio of more than 0.48. Synthesis of 2,6-DMP over the iron-chromium catalyst is characterized by more than 90% degree of phenol conversion. Moreover, the O-alkylation did not occur (which was confirmed by GC-MS analysis). By applying o-cresol circulation in the 2,6-DMP process, selectivity of more than 85% degree of 2,6-DMP was achieved. The participation levels of by-products: 2,4-DMP and 2,4,6-TMP were low. In the optimal conditions based on the highest yield of 2,6-DMP achieved in the technological node applying o-cresol circulation, there are 2%mol. of 2,4-DMP and 6%mol. of 2,4,6-TMP in the final mixture, whereas 2,4,6-TMP can be useful as a chain stopper and polymer's molar mass regulator during the polymerization of 2,6-DMP.

Disorder influence on the magnetic properties of La0.55Sr0.45MnO3/SrTiO3 superlattices

International Nuclear Information System (INIS)

Haberkorn, N.; Sirena, M.; Guimpel, J.; Steren, L.B.

2004-01-01

The structural and physical properties of La 0.55 Sr 0.45 MnO 3 /SrTiO 3 superlattices grown by magnetron sputtering are studied. Two deposition temperatures and different mismatched substrates and buffer layers were used. The structure was determined by refinement through X-ray diffraction pattern fitting. The results indicate 1 unit cell (u.c.) interdiffusion at the interfaces and a 1 u.c. layer thickness fluctuation, i.e. roughness. In-plane hysteresis loops show the expected ferromagnetic behavior, while the perpendicular-to-plane hysteresis loops show anomalies which could evidence magnetic domain size inhomogeneities and stress

Impression management e o desempenho organizacional: o caso português

OpenAIRE

Borges, Maria de Fátima Ribeiro

2011-01-01

O estudo pretende avaliar o impacte do desempenho organizacional na adopção de estratégias de impression management. O estudo apresenta uma análise de conteúdo de 45 cartas do Presidente do Conselho de Administração, contidas nos relatórios e contas anuais de 2010, das empresas Portuguesas não financeiras. Os resultados indicam que o desempenho organizacional não influencia a adoção de estratégias de impression management. Consistente com a teoria psicológica social de im...

Biomass reduction of Salvinia molesta exposed to copper sulfate pentahydrate (CuSO4.5H2O)

OpenAIRE

Barros,João Pedro Alves de Azevedo; Henares,Matheus Nicolino Peixoto

2015-01-01

Copper in the aquatic ecosystem may remain adsorbed or be incorporated into the biomass and undergo biomagnification causing unwanted effects to aquatic macrophyte communities. This study evaluated the biomass reduction of Salvinia molesta (Mitchell) exposed to copper sulphate pentahydrate (CuSO4.5H2O) under laboratory conditions. Approximately 20.5 g of fresh mass (FM) of S. molesta (0.74 g dry matter, DM) were placed in glass tanks with different concentrations (n = 3) of CuSO4.5H2O as foll...

Consumer preferences, internal color and reduction of shigatoxigenic Escherichia coli in cooked hamburgers.

Science.gov (United States)

Røssvoll, Elin; Sørheim, Oddvin; Heir, Even; Møretrø, Trond; Olsen, Nina Veflen; Langsrud, Solveig

2014-02-01

The aim of this study was to relate consumer preferences and preparation of hamburgers to color change, internal temperature and reduction of shigatoxigenic Escherichia coli (STEC) serogroups O157 and the "Big Six" (O26, O45, O103, O111, O121, O145) under two ground beef packaging scenarios: 75% O2 MAP and vacuum. 75% O2 MAP hamburgers cooked to 60 °C core temperature appeared done and showed less internal red color (lower a*) than corresponding vacuum hamburgers. Similar STEC reduction (<4 log10) was found for both hamburgers at core temperatures ≤ 66 °C. In a representative survey (N=1046) most consumers reported to judge hamburger doneness by the color and many preferred undercooked hamburgers. Premature browning of 75% O2 MAP hamburgers represents a risk of foodborne illness, when considering consumers' food handling practices. The risk is even greater if such ground beef is prepared by consumers who prefer undercooked hamburgers and judge doneness by color. © 2013.

Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples

Science.gov (United States)

Krüger, Alejandra; Lucchesi, Paula M. A.; Sanso, A. Mariel; Etcheverría, Analía I.; Bustamante, Ana V.; Burgán, Julia; Fernández, Luciana; Fernández, Daniel; Leotta, Gerardo; Friedrich, Alexander W.; Padola, Nora L.; Rossen, John W. A.

2015-01-01

The Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed that most carried either stx1a or stx2a subtypes. Interestingly, stx2a-positive O26:H11 rarely isolated from cattle in other countries showed to be an important proportion of O26:H11 strains circulating in cattle and food in our region. Seventeen percent of the isolates harbored more than one gene associated with antimicrobial resistance. In addition to stx, all strains contained the virulence genes eae-β, tir, efa, iha, espB, cif, espA, espF, espJ, nleA, nleB, nleC, and iss; and all except one contained ehxA, espP, and cba genes. On the other hand, toxB and espI genes were exclusively observed in stx2-positive isolates, whereas katP was only found in stx1a-positive isolates. Our results show that O26:H11 STEC strains circulating in Argentina, including those isolated from humans, cattle, and meat products, present a high pathogenic potential, and evidence that cattle can be a reservoir of O26:H11 strains harboring stx2a. PMID:26539413

Fauna helmintológica de ovinos provenientes da microrregião de Jaboticabal, estado de São Paulo, Brasil

Directory of Open Access Journals (Sweden)

Willian Giquelin Maciel

2014-03-01

Full Text Available Avaliou-se a prevalência e a contagem parasitária das diferentes espécies de helmintos de ovinos provenientes da microrregião de Jaboticabal, região Noroeste de São Paulo. Para tanto, foram utilizados 66 animais naturalmente infectados, de quatro a 36 meses de idade, criados em regime extensivo. Os resultados necroscópicos revelaram a presença de sete gêneros e 12 espécies, com a seguinte prevalência e a média de parasitismo: Haemonchus contortus: 100,0% (2947,2; Trichostrongylus colubriformis: 90,9% (3048,8; Cooperia curticei: 56,0% (256,5; Oesophagostomum columbianum: 48,4% (36,0; Cooperia punctata: 30,3% (94,5; Trichostrongylus axei: 22,7% (26,5; Strongyloides papillosusi: 19,6% (83,0; Haemonchus contortus (L4: 7,5% (17,2; Cooperia pectinatai: 10,6% (12,9; Trichuris ovis: 10,6% (0,6; Cooperia spatulata 4,5% (0,3; Capillaria bovis: 4,5% (0,1. A carga parasitária média foi de 6.524,7 helmintos por animal. Haemonchus contortus (Adultos e L4 e Trichostrongylus colubriformis corresponderam a 45,4% e 46,7% da carga parasitária média total, respectivamente. Pode-se concluir que as duas espécies de helmintos mais abundantes e importantes da microrregião de Jaboticabal/São Paulo foram Trichostrongylus colubriformis e Haemonchus contortus, sendo que essas duas espécies perfizeram 92,1% da distribuição percentual dos helmintos recolhidos de todos os animais. Tais resultados demonstram a importância em se realizar um monitoramento das contagens de ovos por grama de fezes (OPG dos rebanhos desta região, quando o método FAMACHA for empregado em uma determinada propriedade, uma vez que este método de controle, geralmente, não permite diagnosticar os danos/sinais clínicos desencadeados nos animais pelo T. colubriformis, em função de essa espécie não possuir hábito de hematofagismo sobre os hospedeiros.

Application of WGS data for O-specific antigen analysis and in silico serotyping of Pseudomonas aeruginosa isolates

DEFF Research Database (Denmark)

Thrane, Sandra Wingaard; Taylor, Véronique L.; Lund, Ole

2016-01-01

aeruginosa serotyper (PAst) program, which enabled in silico serotyping of P. aeruginosa isolates using WGS data. PAst has been made publically available as a web-service, and aptly facilitate high-throughput serotyping analysis. The program overcomes critical issues such as the loss of in vitro typeability...... often associated with P. aeruginosa isolates from chronic infections, and quickly determines the serogroup of an isolate based on the sequence of the O-specific antigen (OSA) gene cluster. Here, PAst analysis of 1649 genomes resulted in successful serogroup assignments in 99.27% of the cases...

Ecologia de Tropidurus torquatus (Sauria: Tropiduridae) da Restinga de Guriri, São Mateus, ES

OpenAIRE

TEIXEIRA, R. L.; GIOVANELLI, M.

1999-01-01

Aspectos da ecologia de Tropidurus torquatus foram estudados mensalmente na restinga de Guriri, São Mateus, Norte do Espírito Santo, de fevereiro de 1996 a março de 1997. Foram coletados 108 exemplares com arma de pressão, sendo 45 machos, 56 fêmeas e 7 exemplares cujo sexo não foi identificado. O comprimento rostro-anal no total dos exemplares variou de 26,0 a 101,8 mm (média = 61,7 mm; DP = 15,1). O peso no total dos exemplares variou de 0,8 a 39,6 g (média = 14,6 g; DP = 10,4). Em freqüênc...

Crystal structures of ZnCl2·2.5H2O, ZnCl2·3H2O and ZnCl2·4.5H2O

Directory of Open Access Journals (Sweden)

Erik Hennings

2014-12-01

Full Text Available The formation of different complexes in aqueous solutions is an important step in understanding the behavior of zinc chloride in water. The structure of concentrated ZnCl2 solutions is governed by coordination competition of Cl− and H2O around Zn2+. According to the solid–liquid phase diagram, the title compounds were crystallized below room temperature. The structure of ZnCl2·2.5H2O contains Zn2+ both in a tetrahedral coordination with Cl− and in an octahedral environment defined by five water molecules and one Cl− shared with the [ZnCl4]2− unit. Thus, these two different types of Zn2+ cations form isolated units with composition [Zn2Cl4(H2O5] (pentaaqua-μ-chlorido-trichloridodizinc. The trihydrate {hexaaquazinc tetrachloridozinc, [Zn(H2O6][ZnCl4]}, consists of three different Zn2+ cations, one of which is tetrahedrally coordinated by four Cl− anions. The two other Zn2+ cations are each located on an inversion centre and are octahedrally surrounded by water molecules. The [ZnCl4] tetrahedra and [Zn(H2O6] octahedra are arranged in alternating rows parallel to [001]. The structure of the 4.5-hydrate {hexaaquazinc tetrachloridozinc trihydrate, [Zn(H2O6][ZnCl4]·3H2O}, consists of isolated octahedral [Zn(H2O6] and tetrahedral [ZnCl4] units, as well as additional lattice water molecules. O—H...O hydrogen bonds between the water molecules as donor and ZnCl4 tetrahedra and water molecules as acceptor groups leads to the formation of a three-dimensional network in each of the three structures.

Tellurium sulfates from reactions in oleum and sulfur trioxide: syntheses and crystal structures of TeO(SO_4), Te_4O_3(SO_4)_5, and Te(S_2O_7)_2

International Nuclear Information System (INIS)

Logemann, Christian; Bruns, Joern; Schindler, Lisa Verena; Zimmermann, Vanessa; Wickleder, Mathias S.

2015-01-01

The reaction of K_2TeO_4 with fuming sulfuric acid (65 % SO_3) in sealed glass ampoules at 250 C led to colorless single crystals of TeO(SO_4) [triclinic, P anti 1, Z = 8, a = 819.89(3) pm, b = 836.95(4) pm, c = 1179.12(5) pm, α = 82.820(2) , β = 70.645(2) , γ = 81.897(2) , V = 753.11(6) x 10"6 pm"3]. A horseshoe type [Te_4O_3] fragment is the basic motif in the layer structure of the compound. The [Te_4O_3] moieties are linked to infinite chains by further oxide ions. Monomeric [Te_4O_3] horseshoes are found in the crystal structure of Te_4O_3(SO_4)_5 [trigonal, P3_221, Z = 3, a = 859.05(2) pm, c = 2230.66(7) pm, V = 1425.61(6) x 10"6 pm"3], which was obtained from TeO_2 and fuming sulfuric acid (65 % SO_3) at 200 C as colorless single crystals. By switching to neat SO_3 as reaction medium colorless crystals of Te(S_2O_7)_2 [P2_1/n, Z = 4, a = 1065.25(3) pm, b = 818.50(2) pm, c = 1206.27(3) pm, β = 102.097(1) , V = 1028.40(5) x 10"6 pm"3] form when ortho-telluric acid, H_6TeO_6, is used as the tellurium source. The compound was reported previously, however, obviously with a wrong crystallographic description. In the crystal structure the tellurium atoms are coordinated by two chelating disulfate ions. Further Te-O contacts link the [Te(S_2O_7)_2] units to an extended network. (Copyright copyright 2015 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

Synthesis of O-serogroup specific positive controls and real-time PCR standards for nine clinically relevant non-O157 STECs.

Science.gov (United States)

Conrad, Cheyenne C; Gilroyed, Brandon H; McAllister, Tim A; Reuter, Tim

2012-10-01

Non-O157 Shiga toxin producing Escherichia coli (STEC) are gaining recognition as human pathogens, but no standardized method exists to identify them. Sequence analysis revealed that STEC can be classified on the base of variable O antigen regions into different O serotypes. Polymerase chain reaction is a powerful technique for thorough screening and complex diagnosis for these pathogens, but requires a positive control to verify qualitative and/or quantitative DNA-fragment amplification. Due to the pathogenic nature of STEC, controls are not readily available and cell culturing of STEC reference strains requires biosafety conditions of level 2 or higher. In order to bypass this limitation, controls of stacked O-type specific DNA-fragments coding for primer recognition sites were designed to screen for nine STEC serotypes frequently associated with human infection. The synthetic controls were amplified by PCR, cloned into a plasmid vector and transferred into bacteria host cells. Plasmids amplified by bacterial expression were purified, serially diluted and tested as standards for real-time PCR using SYBR Green and TaqMan assays. Utility of synthetic DNA controls was demonstrated in conventional and real-time PCR assays and validated with DNA from natural STEC strains. Copyright © 2012 Elsevier B.V. All rights reserved.

Microstructure and mechanical properties of in situ TiC and Nd2O3 particles reinforced Ti-4.5 wt.%Si alloy composites

International Nuclear Information System (INIS)

Zhang, Xinjiang; Li, Yibin; Song, Guangping; Sun, Yue; Peng, Qingyu; Li, Yuxin; He, Xiaodong

2011-01-01

Highlights: → (TiC + Nd 2 O 3 )/Ti-4.5 wt.%Si composites were in situ synthesized. → The phase components and microstructures of the composites were investigated. → In situ reinforcements improve the mechanical properties of the matrix alloy. -- Abstract: (TiC + Nd 2 O 3 )/Ti-4.5 wt.%Si composites were in situ synthesized by a non-consumable arc-melting technology. The phases in the composites were identified by X-ray diffraction. Microstructures of the composites were observed by optical microscope and scanning electron microscope. The composite contains four phases: TiC, Nd 2 O 3 , Ti 5 Si 3 and Ti. The TiC and Nd 2 O 3 particles with dendritic and near-equiaxed shapes are well distributed in Ti-4.5 wt.%Si alloy matrix, and the fine Nd 2 O 3 particles exist in the network Ti + Ti 5 Si 3 eutectic cells and Ti matrix of the composites. The hardness and compressive strength of the composites are markedly higher than that of Ti-4.5 wt.%Si alloy. When the TiC content is fixed as 10 wt.% in the composites, the hardness is enhanced as the Nd 2 O 3 content increases from 8 wt.% to 13 wt.%, but the compressive strength peaks at the Nd 2 O 3 content of 8 wt.%.

Synergy effects in mixed Bi2O3, MoO3 and V2O5 catalysts for selective oxidation of propylene

DEFF Research Database (Denmark)

Nguyen, Tien The; Le, Thang Minh; Truong, Duc Duc

2012-01-01

% Bi2Mo3O12 and 78.57 mol% BiVO4), corresponding to the compound Bi1-x/3V1-xMoxO4 with x = 0.45 (Bi0.85V0.55Mo0.45O4), exhibited the highest activity for the selective oxidation of propylene to acrolein. The mixed sample prepared chemically by a sol–gel method possessed higher activity than...

[Two Outbreaks of Yersinia enterocolitica O:8 Infections in Tokyo and the Characterization of Isolates].

Science.gov (United States)

Konishi, Noriko; Ishitsuka, Rie; Yokoyama, Keiko; Saiki, Dai; Akase, Satoru; Monma, Chie; Hirai, Akihiko; Sadamasu, Kenji; Kai, Akemi

2016-01-01

Although the number of outbreaks caused by Yersinia enterocolitica has been very small in Japan, 4 outbreaks were occurred during the 2 years between 2012 and 2013. We describe herein 2 outbreaks which were examined in Tokyo in the present study. Outbreak 1: A total of 39 people (37 high school students and 2 staff) stayed at a hotel in mountain area in Japan had experienced abdominal pain, diarrhea and fever in August, 2012. The Y. enterocolitica serogroup O:8 was isolated from 18 (64.3%) out of 28 fecal specimens of 28 patients. The infection roots could not be revealed because Y. enterocolitica was not detected from any meals at the hotel or its environment. Outbreak 2: A total of 52 students at a dormitory had diarrhea and fever in April, 2013. The results of the bacteriological and virological examinations of fecal specimens of patients showed that the Y. enterocolitica serogroup O:8 was isolated from 24 fecal specimens of 21 patients and 3 kitchen staff. We performed bacteriological and virological examination of the stored and preserved foods at the kitchen of the dormitory to reveal the suspect food. For the detection of Y. enterocolitica, food samples. together with phosphate buffered saline (PBS) were incubated at 4 degrees C for 21 days. Then, a screening test for Y. enterocolitica using realtime-PCR targeting the ail gene was performed against the PBS culture. One sample (fresh vegetable salad) tested was positive on realtime-PCR. No Y. enterocolitica was isolated on CIN agar from the PBS culture because many bacteria colonies other than Y. enterocolitica appeared on the CIN agar. After the alkaline-treatments of the culture broth or the immunomagnetic beads concentration method using anti-Y. enterocolitica O:8 antibodies, Y. enterocolitica O:8 which was the same serogroup as the patients' isolates was successfully isolated from the PBS culture. The fresh vegetable salad was confirmed as the incrimination food of this outbreak.

Produção de forragem e produção animal em pastagem com duas disponibilidades de forragem associadas ou não à suplementação energética Effects of forage availability and energy supplementation on herbage accumulation rate and animal yield

Directory of Open Access Journals (Sweden)

Alcides Pilau

2005-08-01

Full Text Available Neste experimento, avaliou-se o efeito de duas disponibilidades de forragem, 1.200 e 1.500 kg/ha de matéria seca (MS, e do uso de suplementação energética sobre a produção de forragem e a produção animal em pastagem de aveia (Avena strigosa Schreb + azevém (Lolium multiflorum Lam. Foram utilizadas 90 novilhas da raça Charolês e suas cruzas com Nelore, com peso vivo (PV de 164 kg no início do pastejo, submetidas às seguintes combinações: DFB - disponibilidade de forragem baixa; DFA - disponibilidade de forragem alta; DFBS - disponibilidade de forragem baixa + suplementação energética; DFAS - disponibilidade de forragem alta + suplementação energética. O suplemento fornecido foi grão de sorgo moído, na proporção de 0,7% do PV. As variáveis estudadas foram produção de forragem (PF, carga animal (CA e ganho de peso vivo por área (GPA. A PF não foi influenciada pelo manejo da pastagem e pela suplementação aos animais. O acúmulo de forragem diário estimado foi de 45,53 kg/ha de MS. A CA na DFA, média de 862 kg/ha de PV, apresentou pouca variação no decorrer do ciclo de pastejo. Em DFAS, DFB e DFBS, a carga animal foi extremamente variável e, a partir de 26/08, foi maior na DFBS. A utilização de 1.200 kg/ha de MS não afetou o GPA, enquanto a suplementação aos animais proporcionou aumento de 59,4% em relação ao uso exclusivo da pastagem.The trial was conducted to evaluate two forage availabilities (1.200 and 1.500 kg/ha of dry matter [DM] and energy supplementation of winter pasture on herbage and animal yields. The pasture was a mixture of oat (Avena strigosa Schreb + Italian ryegrass (Lolium multiflorum Lam. Ninety Charolais and Charolais crossbred Nellore heifers, with initial live weight of 164 kg, were submitted to the following treatments: LFA - low forage availability; HFA - high forage availability; LFAS - low forage availability + supplementation; HFAS - high forage availability + supplementation

Genetic structure of Neisseria meningitidis serogroup C epidemic strains in South Brazil Estrutura genética de cepas epidêmicas de Neisseria meningitidis sorogrupo C do Sul do Brasil

Directory of Open Access Journals (Sweden)

Claudio Tavares Sacchi

1995-08-01

Full Text Available In the present study we report the results of an analysis, based on serotyping, multilocus enzyme electrophoresis (MEE, and ribotyping of N. meningitidis serogroup C strains isolated from patients with meningococcal disease (MD in Rio Grande do Sul (RS and Santa Catarina (SC States, Brazil, as the Center of Epidemiology Control of Ministry of Health detected an increasing of MD cases due to this serogroup in the last two years (1992-1993. We have demonstrated that the MD due to N.meningitidis serogroup C strains in RS and SC States occurring in the last 4 years were caused mainly by one clone of strains (ET 40, with isolates indistinguishable by serogroup, serotype, subtype and even by ribotyping. One small number of cases that were not due to an ET 40 strains, represent closely related clones that probably are new lineages generated from the ET 40 clone referred as ET 11A complex. We have also analyzed N.meningitidis serogroup C strains isolated in the greater São Paulo in 1976 as representative of the first post epidemic year in that region. The ribotyping method, as well as MEE, could provide useful information about the clonal characteristics of those isolates and also of strains isolated in south Brazil. The strains from 1976 have more similarity with the actual endemic than epidemic strains, by the ribotyping, sulfonamide sensitivity, and MEE results. In conclusion, serotyping with monoclonal antibodies (C:2b:P1.3, MEE (ET 11 and ET 11A complex, and ribotyping by using ClaI restriction enzyme (Rb2, were useful to characterize these epidemic strains of N.meningitidis related to the increased incidence of MD in different States of south Brazil. It is mostly probable that these N.meningitidis serogroup C strains have poor or no genetic corelation with 1971-1975 epidemic serogroup C strains. The genetic similarity of members of the ET 11 and ET 11A complex were confirmed by the ribotyping method by using three restriction endonucleases

RELAÇÃO ENTRE O ESTADO PSICOSSOCIAL DO CUIDADOR INFORMAL E O TEMPO DE CUIDADO DOS IDOSOS DA REGIÃO CENTRO DE PORTUGAL

Directory of Open Access Journals (Sweden)

Ricardo Pocinho

2017-06-01

Full Text Available O papel desempenhado pela família e cuidadores no tratamento dos idosos é essencial para a promoção e manutenção da sua qualidade de vida, garantindo a sua manutenção do domicílio por um período de tempo mais alargado. Objetivo: Compreender a existência da sobrecarga do cuidador informal e os fatores associados (apoio disponíveis e perfil, bem como estudar o estabelecimento relacional entre o tempo e a sobrecarga. Metodologia: Um questionário de dados sociodemográficos e QASCI (Questionário de Avaliação da Sobrecarga do Cuidador Informal aplicado a 30 cuidadores da região centro de Portuga. Resultados: São caraterísticas do perfil do cuidador, 45 anos, casado, existência de coabitação e razões relacionadas com o dever e a ligação emocional subjacente.

Hierarchical CNFs/MnCo2O4.5 nanofibers as a highly active oxidase mimetic and its application in biosensing

Science.gov (United States)

Gao, Mu; Lu, Xiaofeng; Nie, Guangdi; Chi, Maoqiang; Wang, Ce

2017-12-01

Recently, much attention has been paid on the nanomaterial-based artificial enzymes due to their tunable catalytic activity, high stability and low cost compared to the natural enzymes. Different from the peroxidase mimics which have been studied for several decades, nanomaterials with oxidase-like property are burgeoning in the recent years. In this paper, hierarchical carbon nanofibers (CNFs)/MnCo2O4.5 nanofibers as efficient oxidase mimics are reported. The products are synthesized by an electrospinning technique and an electrochemcial deposition process in which the CNFs are used as the working electrode where MnCo2O4.5 nanosheets deposit on. The resulting binary metal oxide-based nanocomposites exhibit a good oxidase-like activity toward the oxidations of 3,3‧,5,5‧tetramethylbenzi-dine (TMB), 2,2‧-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) salt and o-phenylenediamine (OPD) without exogenous addition of H2O2. The system of CNFs/MnCo2O4.5-TMB can be used as a candidate to detect sulfite and ascorbic acid via a colorimetric method with a high sensitivity. This work provides the efficient utilization and potential applications of binary metal oxide-based nanocomposites with oxidase activities in biosensors and other biotechnologies.

A doença meningocócica na região de Sorocaba, São Paulo, Brasil, no período de 1999 a 2008 Meningococcal disease in the Sorocaba region, São Paulo State, Brazil, 1999-2008

Directory of Open Access Journals (Sweden)

Miriam Vannucchi Leme

2012-12-01

Full Text Available O objetivo do estudo foi descrever a ocorrência da doença meningocócica notificada no Departamento Regional de Saúde XVI, Sorocaba, São Paulo, Brasil, no período de 1999 a 2008. Em Sorocaba, a incidência anual da doença foi de cerca de dois casos por 100 mil habitantes, com aumento entre 2006 e 2008. As incidências anuais foram maiores entre 0 a 4 anos de idade. A letalidade no período foi de 21,8%, maior nas faixas etárias de 0 a 4 anos (26,4%, na qual se deu a maior incidência da doença, e com idade superior a 30 anos (28%. A confirmação diagnóstica foi laboratorial em 71% dos casos (cultura em 45,3% e por critérios clínico-epidemiológicos em 22%. O sorogrupo B ocorreu em 45,7%; o C, em 47,3%; o W135, em 3,7%; e o Y, em 1,5% dos casos identificados, com predomínio do sorogrupo B, entre 1999 e 2003, e do C, entre 2004 e 2008. Os fenótipos B:4,7:P1.19,15 e C:23:P1.14-6 predominaram. Os resultados reforçam a necessidade de acompanhamento, de forma regional, da tendência da doença para a detecção de surtos precocemente e monitoramento de cepas circulantes.The objective of this study was to describe the occurrence of meningococcal disease reported to the Regional Health Department in Sorocaba, São Paulo State, Brazil, from 1999 to 2008. Annual incidence of the disease was two cases per 100,000 inhabitants, with an increase from 2006 to 2008. Annual incidence rates were highest in the 0 to 4 year age bracket. Case-fatality was 21.8%, higher in the 0 to 4 year age bracket (26.4%, which also showed the highest incidence of the disease, and in the over 30-year age bracket (28%. Diagnosis was confirmed by laboratory test in 71% of cases (culture in 45.3% and by clinical and epidemiological criteria in 22%. Serological groups were B in 45.7%, C in 47.3%, W135 in 3.7%, and Y in 1.5% of the identified cases, with a predominance of B from 1999 to 2003 and C from 2004 to 2008. The most frequent phenotypes were B:4,7:P1.19,15 and C

Efeito do pH na adsorção-dessorção de chumbo em Latossolos brasileiros

Directory of Open Access Journals (Sweden)

M. A. P. Pierangeli

2001-06-01

Full Text Available Reações de adsorção-dessorção de chumbo em solos são influenciadas por atributos de superfície dos colóides dos solos e pela composição da solução do meio. Este estudo avaliou o efeito do pH sobre a adsorção-dessorção de chumbo em Latossolos brasileiros. Amostras do horizonte A de cada solo, suspensas em Ca(NO32 5 mmol L-1, foram tituladas com HNO3 7 mmol L-1 ou solução saturada de Ca(OH2, para que fosse atingido o valor de pH estipulado em cada experimento (4,5, 5,5 e 6,5. Atingido o pH de equilíbrio, as amostras foram equilibradas com Pb(NO32, com vistas em obter uma concentração final de 0,15 mmol L-1 (relação solo:solução 1:100; força iônica 15 mmol L-1, por um período de 72 h. A dessorção foi realizada em Ca(NO32 5 mmol L-1, pH 5,5. O aumento do pH de 4,5 para 6,5 causou aumento da adsorção de até 16,7 vezes, um aumento médio de 2,9 vezes de pH 4,5 para 5,5; 1,4 vez de pH 5,5 para 6,5 e 4,2 vezes de pH 4,5 para 6,5. A relação Pb adsorvido/Pb adicionado foi, em média, de 0,33 para pH 4,5; 0,75 para pH 5,5 e 0,94 para pH 6,5. A fração média de Pb dessorvido (Pb dessorvido/Pb adsorvido decresceu de 0,36 a pH 4,5, para < 0,06 a pH 6,5. O efeito dos atributos do solo sobre a adsorção-dessorção de chumbo decresceu quando o pH aumentou, evidenciado por uma maior diferenciação na quantidade adsorvida pelos solos em valores mais baixos de pH. A adsorção de Pb foi positivamente correlacionada (e geralmente a fração dessorvida foi negativamente correlacionada com área superficial específica, CTC a pH 7,0, teores de caulinita, hematita, Fe2O3 extraídos pelo ditionito-citrato-bicarbonato de sódio e oxalato ácido de amônio e SiO2 e Fe2O3 extraídos pelo ataque sulfúrico. O fato de considerável fração de chumbo permanecer adsorvida em pH 4,5 mostra a reduzida disponibilidade deste metal em Latossolos, mesmo em baixos valores de pH.

Teores de cálcio em dietas para codornas japonesas no terço final de postura (45 a 57 semanas de idade Levels of calcium in diets for Japanese quails in the third end of posture (45 - 57 weeks of age

Directory of Open Access Journals (Sweden)

C.H.R. Costa

2010-10-01

Full Text Available Verificou-se o efeito dos teores de cálcio (Ca na dieta sobre o desempenho e a qualidade dos ovos de codornas japonesas durante o terço final do ciclo de produção. Foram utilizadas 350 codornas, com 313 dias de idade, distribuídas em 35 unidades experimentais de 10 aves. O delineamento foi inteiramente ao acaso, composto de cinco níveis de Ca - 2,2; 2,6; 3,0; 3,4; 3,8% -, com sete repetições. O aumento do Ca na dieta melhorou linearmente a conversão por massa e por dúzia de ovos, melhorou a espessura de casca e influenciou o peso da gema e da casca dos ovos, além da porcentagem da casca. Observou-se redução nos teores de P, Ca e Mg na casca com o aumento dos níveis de Ca da dieta. Para os demais parâmetros, não houve efeito do teor de Ca da dieta. Considerando-se a importância da conversão alimentar, em termos econômicos e de desempenho, o teor de 3,8% de Ca na dieta mostrou-se satisfatório em atender às demais variáveis relacionadas com a qualidade dos ovos. Dieta que contenha 3,8% de Ca, correspondendo ao consumo diário de 982mg por ave, melhora a conversão alimentar e a qualidade da casca dos ovos de codornas japonesas no terço final do ciclo de produção (45 a 57 semanas de idade.The effect of calcium (Ca levels in the diet on performance and egg quality of Japanese quail during the final third of the production cycle was evaluated. A total of 350 313-day-old quails was used. They were distributed in 35 experimental units of 10 birds. The design was completely randomized, composed of five levels of Ca (2.2, 2.6, 3.0, 3.4 and 3.8%, with seven replications. The increase of Ca in the diet linearly improved the feed conversion per dozen eggs, improved the shell thickness, and influenced the weight of yolk and egg shell as well the percentage of shell. There was reduction in levels of P, Ca, and Mg in the shell with increasing levels of Ca in the diet. For other parameters, no effect of calcium levels in the diet was

Investigations of structural, morphological and optical properties of Cu:ZnO/TiO2/ZnO and Cu:TiO2/ZnO/TiO2 thin films prepared by spray pyrolysis technique

Directory of Open Access Journals (Sweden)

M.I. Khan

Full Text Available The aim of this research work is presented a comparison study of Cu:ZnO/TiO2/ZnO (Cu:ZTZ and Cu:TiO2/ZnO/TiO2 (Cu:TZT thin films deposited by spray pyrolysis technique on FTO substrates. After deposition, these films are annealed at 500 °C. XRD confirms the anatase phase of TiO2 and Hexagonal wurtzite phase of ZnO. SEM shows that Cu:TZT has more porous surface than Cu:ZTZ and also the root mean square (RMS roughness of Cu:TZT film is 48.96 and Cu:ZTZ film is 32.69. The calculated optical band gaps of Cu:TZT and Cu:ZTZ thin films are 2.65 eV and 2.6 eV respectively, measured by UV–Vis spectrophotometer. This work provides an environment friendly and low cost use of an abundant material for highly efficient dye sensitized solar cells (DSSCs. Keywords: Multilayer films, ZnO, TiO2, Cu

A study of Love wave devices in ZnO/Quartz and ZnO/LiTaO3 structures

International Nuclear Information System (INIS)

Chang, R.-C.; Chu, S.-Y.; Hong, C.-S.; Chuang, Y.-T.

2006-01-01

Love wave devices are very promising for sensing applications because of high sensitivity. In this paper, ZnO thin films doped with lithium (Li) and magnesium (Mg) were deposited on the 42 o 45' ST-cut quartz and 36 o YX-LiTaO 3 substrates by RF magnetron sputtering technique. XRD, SEM, and AFM measurements investigated characteristics of the films. Under different conditions such as doping content, layer thickness, and substrate temperature, the phase velocity, temperature coefficient of frequency, electromechanical coupling coefficient and sensitivity of Love wave devices in ZnO/Quartz and ZnO/LiTaO 3 structures are presented. The maximum sensitivities of ZnO/Quartz and ZnO/LiTaO 3 are much higher than the SiO 2 /Quartz and SiO 2 /LiTaO 3 structures reported

Study of the reversible water vapour sorption process of MgSO4.7H2O and MgCl2.6H2O under the conditions of seasonal solar heat storage

NARCIS (Netherlands)

Ferchaud, C.; Zondag, H.A.; Veldhuis, J.B.J.; Boer, de R.

2012-01-01

The characterization of the structural, compositional and thermodynamic properties of MgSO4.7H2O and MgCl2.6H2O has been done using in-situ X-ray Diffraction and thermal analyses (TG/DSC) under practical conditions for seasonal heat storage (Tmax=150°C, p(H2O)=13 mbar). This study showed that these

6-Peroxo-6-zirconium crown and its hafnium analogue embedded in a triangular polyanion: [M6(O2)6(OH)6(gamma-SiW10O36)3]18- (M = Zr, Hf).

Science.gov (United States)

Bassil, Bassem S; Mal, Sib Sankar; Dickman, Michael H; Kortz, Ulrich; Oelrich, Holger; Walder, Lorenz

2008-05-28

We have synthesized and structurally characterized the unprecedented peroxo-zirconium(IV) containing [Zr6(O2)6(OH)6(gamma-SiW10O36)3]18- (1). Polyanion 1 comprises a cyclic 6-peroxo-6-zirconium core stabilized by three decatungstosilicate units. We have also prepared the isostructural hafnium(IV) analogue [Hf6(O2)6(OH)6(gamma-SiW10O36)3]18- (2). We investigated the acid/base and redox properties of 1 by UV-vis spectroscopy and electrochemistry studies. Polyanion 1 represents the first structurally characterized Zr-peroxo POM with side-on, bridging peroxo units. The simple, one-pot synthesis of 1 and 2 involving dropwise addition of aqueous hydrogen peroxide could represent a general procedure for incorporating peroxo groups into a large variety of transition metal and lanthanide containing POMs.

Fluência digital e uso de ambientes virtuais: caracterização de alunos de enfermagem

Directory of Open Access Journals (Sweden)

Priscila Berenice Costa

2011-12-01

Full Text Available O objetivo do estudo foi caracterizar o perfil de licenciandos de enfermagem, identificar a fluência digital, o conhecimento, a habilidade e o interesse no uso de ambientes virtuais de aprendizagem. Trata-se de uma pesquisa quantitativa, exploratório-descritiva realizada com 51 licenciandos de enfermagem por meio de questionário. Dos participantes do estudo 51 (100% afirmam ter conhecimento em informática, sendo que 26 (49% indicam um nível intermediário; 47 (92% fazem uso diário da Internet; 51 (100% navegam em redes sociais e possuem e-mail; 51(100% utilizam MSN e 32 (62,7% Skype; 41 (82% acessam Chats, 33 (64,7% Fóruns de discussão e 22 (43% Blogs; 33 (64,7% utilizam frequentemente o Moodle e 26 (51% o COL; e a grande maioria (45- 88,2% relatou interesse no uso de ambientes virtuais de aprendizagem. Os alunos foram considerados fluentes digitais e apresentaram conhecimento, habilidade e expressivo interesse no uso de ambientes virtuais de aprendizagem em sua formação acadêmica.

Study of the reversible water vapour sorption process of MgSO{sub 4}.7H{sub 2}O and MgCl{sub 2}.6H{sub 2}O under the conditions of seasonal solar heat storage

Energy Technology Data Exchange (ETDEWEB)

Ferchaud, C.J.; De Boer, R. [Eindhoven University of Technology, Department of Mechanical Engineering, Eindhoven (Netherlands); Zondag, H.A.; Veldhuis, J.B.J. [ECN Biomass and Energy Efficiency, Petten (Netherlands)

2012-08-15

The characterization of the structural, compositional and thermodynamic properties of MgSO{sub 4}.7H{sub 2}O and MgCl{sub 2}.6H{sub O} has been done for seasonal heat storage using in-situ X-ray Diffraction and thermal analyses (TG/DSC) under practical conditions for seasonal heat storage (T{sub max} = 150C, p(H{sub 2}O)=13 mbar). This study showed that these two materials release heat after a dehydration/hydration cycle with energy densities of 0.38 GJ/m{sup 3} for MgSO{sub 4}.7H{sub 2}O and 0.71 GJ/m{sup 3} MgCl{sub 2}.6H{sub 2}O. The low heat release found for MgSO{sub 4}.7H2O is mainly attributed to the amorphization of the material during the dehydration performed at 13 mbar which reduces its sorption capacity during the rehydration. MgCl{sub 2}.6H{sub 2}O presents a high energy density which makes this material interesting for the seasonal heat storage in domestic applications. This material would be able to fulfil the winter heat demand of a passive house estimated at 6 GJ with a packed bed reactor of 8.5 m{sup 3}. However, a seasonal heat storage system based on the water vapour sorption process in MgCl{sub 2}.6H{sub 2}O should be carefully set with a restricted temperature of 40C for the hydration reaction to avoid the liquefaction of the material at ambient temperature which limits its performances for long term storage.

Phase formation in K2O(K2CO3)-CdO-MoO3 system

International Nuclear Information System (INIS)

Tsirenova, G.D.; Tsybikova, B.A.; Bazarova, Zh.G.; Solodovnikov, S.F.; Zolotova, E.S.

2000-01-01

Phase formation in K 2 O(K 2 CO 3 )-CdO-MoO 3 system are studied by the methods of x-ray diffraction, thermal and crystal optical analyses. Three potassium-cadmium molybdates are detected: K 4 Cd(MoO 4 ) 3 with a new structure, alluodite-like K 4-2x Cd 1+x (MoO 4 ) 3 (0.26≤x≤0.38 at 470 Deg C) and K 4 CdMo 4 O 15 of K 4 MnMo 4 O 15 type. First of them decomposes in solid phase at 580 Deg C, and others melt incongruently at 720 and 515 Deg C correspondingly. It is established that K 4-2x Cd 1+x (MoO 4 ) 3 compound undergoes phase transition of the second type in the temperature interval of 500-550 Deg C. Phase diagram of quasibinary cross section K 2 MoO 4 -CdMoO 4 is plotted [ru

Electric Properties of Pb(Sb1/2Nb1/2)O3 PbTiO3 PbZrO3 Ceramics

Science.gov (United States)

Kawamura, Yasushi; Ohuchi, Hiromu

1994-09-01

Solid-solution ceramics of ternary system xPb(Sb1/2Nb1/2)O3 yPbTiO3 zPbZrO3 were prepared by the solid-state reaction of powder materials. Ceramic, electric, dielectric and piezoelectric properties and crystal structures of the system were studied. Sintering of the system xPb(Sb1/2Nb1/2)O3 yPbTiO3 zPbZrO3 is much easier than that of each end composition, and well-sintered high-density ceramics were obtained for the compositions near the morphotropic transformation. Piezoelectric ceramics with high relative dielectric constants, high radial coupling coefficient and low resonant resistance were obtained for the composition near the morphotropic transformation. The composition Pb(Sb1/2Nb1/2)0.075Ti0.45Zr0.475O3 showed the highest dielectric constant (ɛr=1690), and the composition Pb(Sb1/2Nb1/2)0.05Ti0.45Zr0.5O3 showed the highest radial coupling coefficient (kp=64%).

Desempenho de cultivares de cebola em cultivo orgânico e tipos de solo no Vale do São Francisco Evaluation of onion cultivars under organic cultivation in two soil types in the São Francisco Valley, Brazil

Directory of Open Access Journals (Sweden)

Nivaldo D Costa

2008-12-01

Full Text Available Com o objetivo de avaliar a produtividade de cultivares de cebola em cultivo orgânico no Vale do São Francisco, conduziram-se dois experimentos, de maio a outubro de 2005, nos Campos Experimentais de Bebedouro, Petrolina-PE, e Mandacaru, Juazeiro-BA, em ARGISSOLO e VERTISSOLO respectivamente. O delineamento experimental utilizado foi em blocos ao acaso, utilizando-se dezoito e quatorze cultivares de cebola, respectivamente e quatro repetições. Em ARGISSOLO a produtividade total de bulbos variou de 13,52 a 39,52 t/ha. A produtividade comercial oscilou de 7,45 a 38,32 t/ha, sobressaindo-se como mais produtivas as cultivares Brisa IPA-12 (38,32 t/ha e São Paulo (35,86 t/ha que não evidenciaram diferenças significativas entre si, assim como as cultivares Botucatu-150 (26,41 t/ha e Pira Ouro (26,37 t/ha, e menos produtivas as cultivares Conquista (7,45 t/ha e Crioula Alto Vale (7,81 t/ha. Em VERTISSOLO a produtividade total de bulbos variou de 6,87 a 24,68 t/ha. Sobressairam-se com produtividade comercial as cultivares Texas Grano PRR (21,56 t/ha e IPA-10 (17,50 t/ha, que não diferiram entre si. As cultivares Crioula Alto Vale, CNPH-6348, CNPH- 6436 e CNPH-6206 não tiveram produção comercial de bulbos.In this research were evaluated the yield of onion cultivars under organic growth in the São Francisco Valley. Two field trials were evaluated, one at the Experimental Farm Station of Embrapa Tropical Semi-Arid, in Petrolina, in an ultisol type and the other at the Experimental Farm Station of Mandacaru, in Juazeiro, in a vertisol type, from February to October of 2005. The experimental design was of randomized complete blocks, with 18 and 14 cultivars, respectively, and four replications. In the ultisol, the total yield of bulbs ranged from 13.52 to 39.52 t/ha. The commercial yield ranged from 7.45 to 38.32 t/ha where the cultivars Brisa (38.32 t/ha and São Paulo (35.86 t/ha showed the highest yield without statistical difference

Diversity of Shiga Toxin-Producing Escherichia coli (STEC) O26:H11 Strains Examined via stx Subtypes and Insertion Sites of Stx and EspK Bacteriophages

Science.gov (United States)

Bonanno, Ludivine; Loukiadis, Estelle; Mariani-Kurkdjian, Patricia; Oswald, Eric; Garnier, Lucille; Michel, Valérie

2015-01-01

Shiga toxin-producing Escherichia coli (STEC) is a food-borne pathogen that may be responsible for severe human infections. Only a limited number of serotypes, including O26:H11, are involved in the majority of serious cases and outbreaks. The main virulence factors, Shiga toxins (Stx), are encoded by bacteriophages. Seventy-four STEC O26:H11 strains of various origins (including human, dairy, and cattle) were characterized for their stx subtypes and Stx phage chromosomal insertion sites. The majority of food and cattle strains possessed the stx1a subtype, while human strains carried mainly stx1a or stx2a. The wrbA and yehV genes were the main Stx phage insertion sites in STEC O26:H11, followed distantly by yecE and sbcB. Interestingly, the occurrence of Stx phages inserted in the yecE gene was low in dairy strains. In most of the 29 stx-negative E. coli O26:H11 strains also studied here, these bacterial insertion sites were vacant. Multilocus sequence typing of 20 stx-positive or stx-negative E. coli O26:H11 strains showed that they were distributed into two phylogenetic groups defined by sequence type 21 (ST21) and ST29. Finally, an EspK-carrying phage was found inserted in the ssrA gene in the majority of the STEC O26:H11 strains but in only a minority of the stx-negative E. coli O26:H11 strains. The differences in the stx subtypes and Stx phage insertion sites observed in STEC O26:H11 according to their origin might reflect that strains circulating in cattle and foods are clonally distinct from those isolated from human patients. PMID:25819955

Estudo de 26 casos de Paracoccidioidomicose avaliados no Serviço de Otorrinolaringologia da Fundação Oswaldo Cruz (FIOCRUZ A study of 26 cases of Paracoccidioidomycosis evaluated at the Oswaldo Cruz Foundation (FIOCRUZ Otorhinolaryngology Service

Directory of Open Access Journals (Sweden)

Francisco X. Palheta-Neto

2003-10-01

Full Text Available A Paracoccidioidomicose (PCM é uma micose profunda sistêmica causada por um fungo dimorfo, de aspecto microscópico similar a roda de leme de navio, o Paracoccidioides brasiliensis (Pb. A PCM apresenta distribuição geográfica restrita ao continente americano, acomete sobretudo indivíduos do sexo masculino, sem predileção por raça. Seu quadro clínico é bastante variado. OBJETIVO: Realizar análise epidemiológica de 26 casos de PCM avaliados no Setor de Otorrinolaringologia da Fundação Oswaldo Cruz - FIOCRUZ. FORMA DE ESTUDO: Clínico retrospectivo. MATERIAL E MÉTODO: Estudou-se retrospectivamente 26 prontuários de pacientes com manifestações otorrinolaringológicas da PCM atendidos no período de outubro de 1998 a setembro de 2001. Avaliou-se: sexo, idade, profissão, grau de escolaridade, se etilistas e/ou tabagistas, sintomatologia, local das lesões, presença de co-infecção, exames complementares realizados e opção terapêutica. RESULTADOS: 23 (88,5% pacientes eram do sexo masculino e 3 (11,5% do sexo feminino, com idade entre 32 e 72 anos. 11 (42,3% pacientes eram agricultores e 21 (80,8% tabagistas e/ou etilistas. Os locais mais comumente atingidos foram a epiglote e a parede nasal lateral. Utilizaram-se derivados sulfamídicos para o tratamento de 14 (53,9% pacientes e imidazólicos em 12 (46,1% casos. DISCUSSÃO: Lesões pulmonares podem ser confundidas com tuberculose, devido a suas semelhanças clínicas e radiográficas. A desnutrição pode predispor quadros clínicos de extrema gravidade. CONCLUSÃO: A anamnese detalhada dos pacientes portadores de PCM revela importantes aspectos, como procedência e hábitos pessoais. A atuação interdisciplinar, sobretudo de médicos e dentistas, colabora para um precoce diagnóstico e adequado acompanhamento do doente.Paracoccidioidomycosis (PCM is an acute systemic mycosis caused by a dimorphous fungus, Paracoccidioides brasiliensis (Pb, whose appearance under the

Organic-inorganic hybrid supramolecular assemblies based on isomers [H{sub x}As{sub 2}Mo{sub 6}O{sub 26}]{sup (6-x)-} clusters

Energy Technology Data Exchange (ETDEWEB)

Zhao, Zhi-Feng [College of Material Science and Engineering, Heilongjiang University of Science and Technology, Harbin (China); Su, Zhan-Hua; Cong, Bo-Wen; Zhao, Wen-Qi; Ma, Xiu-Juan [School of Chemistry and Chemical Engineering, Harbin Normal University, Harbin (China)

2017-08-16

The arsenomolybdates [H{sub 2}As{sub 2}Mo{sub 6}O{sub 26}(H{sub 2}O)].(H{sub 2}biyb){sub 2}.2H{sub 2}O (1) and [H{sub 3}As{sub 2}Mo{sub 6}O{sub 26}].(H{sub 3}pt){sub 2} (2) [biyb = 1,4-bis(imidazol-1-ylmethyl)benzene, pt = 4'-(3{sup ''}-pyridyl)-2,3':6'3{sup ''}-terpyridine] were synthesized via hydrothermal method. The structures of the compounds were characterized by single-crystal X-ray diffraction analyses, elemental analyses, IR spectroscopy, and TG analysis. Compounds 1 and 2 exhibit two isomeric forms of [H{sub x}As{sub 2}Mo{sub 6}O{sub 26}]{sup (6-x)-}. The structure of 1 is constructed from the B-type [H{sub 2}As{sub 2}Mo{sub 6}O{sub 26}(H{sub 2}O)]{sup 4-} polyanions and free biyb ligands via weak interactions to form 3D supramolecular framework with a {3.4.5"3.6}{3.4"3.5"2}{3.5.6}{sup 2}{3.5"2}{sup 2} topology structure. In compound 2, the A-type [H{sub 3}As{sub 2}Mo{sub 6}O{sub 26}]{sup 3-} clusters are surrounded by pt ligands through hydrogen bond interactions forming 3D supramolecular framework with a {4"3.6"3}{sup 2}{4"6.6"6.8"3} topology structure. The electrochemical behaviors, electrocatalytic and photocatalytic activities of 1 and 2 are detected. (copyright 2017 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

45 CFR 99.26 - Unsponsored written material.

Science.gov (United States)

2010-10-01

... 45 Public Welfare 1 2010-10-01 2010-10-01 false Unsponsored written material. 99.26 Section 99.26 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION PROCEDURE FOR HEARINGS FOR THE CHILD CARE AND DEVELOPMENT FUND Hearing Procedures § 99.26 Unsponsored written material. Letters...

45 CFR 1174.26 - Non-Federal audit.

Science.gov (United States)

2010-10-01

... 45 Public Welfare 3 2010-10-01 2010-10-01 false Non-Federal audit. 1174.26 Section 1174.26 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS AND THE....26 Non-Federal audit. (a) Basic rule. Grantees and subgrantees are responsible for obtaining audits...

45 CFR 1183.26 - Non-Federal audit.

Science.gov (United States)

2010-10-01

... 45 Public Welfare 3 2010-10-01 2010-10-01 false Non-Federal audit. 1183.26 Section 1183.26 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS AND THE....26 Non-Federal audit. (a) Basic rule. Grantees and subgrantees are responsible for obtaining audits...

45 CFR 602.26 - Non-Federal audit.

Science.gov (United States)

2010-10-01

... 45 Public Welfare 3 2010-10-01 2010-10-01 false Non-Federal audit. 602.26 Section 602.26 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION UNIFORM... Requirements § 602.26 Non-Federal audit. (a) Basic rule. Grantees and subgrantees are responsible for obtaining...

Prevalência dos HPV 16, 18, 45 e 31 em mulheres com lesão cervical Prevalence of HPV 16, 18, 45 and 31 in women with cervical lesions

Directory of Open Access Journals (Sweden)

Denise Rocha Pitta

2010-07-01

Full Text Available OBJETIVO: avaliar a prevalência dos HPV 16, 18, 31 e 45 em amostras de raspado cervical de mulheres com alterações celulares e/ou colposcopia sugestiva de lesão de alto grau ou lesão de baixo grau persistente submetidas à conização. MÉTODOS: Foram incluídas 120 mulheres. A análise histológica dos cones cervicais revelou 7 casos de cervicite, 22 de NIC1, 31 de NIC2, 54 de NIC3 e 6 carcinomas invasores. Foram analisadas as amostras de raspado cervical coletadas antes da conização para a presença do DNA-HPV por PCR com os primers de consenso, PGMY09/11. As amostras positivas para DNA de HPV foram testadas para presença do HPV16, 18, 31 e 45 utilizando-se primers tipo específico para esses HPV. RESULTADOS: O DNA-HPV foi detectado em 67,5% das mulheres. O HPV 16 (40% foi o tipo mais prevalente na maioria das lesões, seguido dos HPV 31 (13,3%, 45 (13,3% e 18 (4,1%. Infecções múltiplas ocorreram em 15% dos casos e as infecções por outros tipos de HPV foram detectadas em 14% da amostra. CONCLUSÕES: as infecções pelos HPV 16 e 18 nem sempre ocorrem de maneira solitária (infecção única, estando associadas a outros tipos de HPV em diversas ocasiões.PURPOSE: to determine the prevalence of HPV 16, 18, 31 and 45 in cervical screening samples of women with cellular changes and/or colposcopy suggestive of persistent high grade or low grade lesion who were submitted to conization. METHODS: a total of 120 women were included in the study. Histological analysis of the cervical cones revealed 7 cases of cervicitis, 22 of CIN1, 31 of CIN2, 54 of CIN3, and 6 invasive carcinomas. The cervical screening samples were analyzed before conization for the presence of HPV-DNA by PCR using the consensus primers PGMY09/11. HPV-DNA-positive samples were tested for the presence of HPV16, 18, 31 and 45 using type-specific primers for these HPV. RESULTS: HPV-DNA was detected in 67.5% of the studied women. HPV 16 (40% was the most prevalent type in

Ocorrência de parasitos gastrintestinais em fezes de gatos das cidades de São Paulo e Guarulhos

Directory of Open Access Journals (Sweden)

Alessandra Mara Alves Ragozo

2002-01-01

Full Text Available Amostras de fezes foram colhidas de 138 gatos, de idade, sexo e raça variadas, capturados nas ruas das cidades de São Paulo e Guarulhos, para determinação da presença de parasitos gastrintestinais. Os animais encontravam-se alojados individualmente no Centro de Controle de Zoonoses dos Municípios de São Paulo (107 gatos e Guarulhos (31gatos. As fezes foram colhidas individualmente e processadas pela técnica de flutuação em solução de sacarose (d=1,203g/cm³. Dentre os protozoários o agente mais freqüente foi o Cystoisospora felis, em 36 gatos (26,09%, seguido pelo Cystoisospora rivolta, em 34 (24,64%, pelo Cryptosporidium parvum, em dois (1,45% e pelo Sarcocystis spp. em um animal (0,72%. Dentre os helmintos, Toxocara cati foi o de maior ocorrência, com 39 gatos positivos (28,26%, seguido pelo Ancylostoma spp., com 12 gatos positivos (8,70% e pelo Platynosomun fastosum em dois gatos (1,45%. A presença de proglotes de Dipylidium caninum foi observada em duas amostras, quando da colheita nas gaiolas, dado este certamente subestimado, uma vez que o encontro de cápsula ovígera em fezes é bastante raro, sendo o diagnóstico feito pela observação de proglotes nas fezes frescas. Os parasitos estavam presentes em infecções múltiplas em 25 animais (18,12% sendo as ocorrências mais comuns T.cati com Cystoisospora spp. e T.cati com Ancylostoma spp., ambas com 7,97% de ocorrência (11 amostras.

Comparative study of phase structure and dielectric properties for K0.5Bi0.5TiO3-BiAlO3 and LaAlO3-BiAlO3

International Nuclear Information System (INIS)

Hou, Yudong; Zheng, Mupeng; Si, Meiju; Cui, Lei; Zhu, Mankang; Yan, Hui

2013-01-01

In this work, two perovskite-type compounds, K 0.5 Bi 0.5 TiO 3 and LaAlO 3 , have been selected as host material to incorporate with BiAlO 3 using a solid-state reaction route. The phase evolution and dielectric properties for both systems have been investigated in detail. For the K 0.5 Bi 0.5 TiO 3 -BiAlO 3 system, it is interesting to find that when using Bi 2 O 3 , Al 2 O 3 , K 2 CO 3 , and TiO 2 as starting materials, the formed compounds are K 0.5 Bi 0.5 TiO 3 -K 0.5 Bi 4.5 Ti 4 O 15 and Al 2 O 3 only plays a dopant role. There are two distinct dielectric peaks appearing in the patterns of temperature dependence of dielectric constant, corresponding to the phase-transition points of perovskite-type K 0.5 Bi 0.5 TiO 3 and Aurivillius-type K 0.5 Bi 4.5 Ti 4 O 15 , independently. In comparison, using Bi 2 O 3 , Al 2 O 3 , and La 2 O 3 as starting materials, the pure perovskite phase LaAlO 3 -BiAlO 3 can be obtained. Compared to the inherent paraelectric behavior in LaAlO 3 , the diffuse phase-transition phenomena can be observed in the LaAlO 3 -BiAlO 3 binary system, which corresponds well to the Vogel-Fulcher (VF) relationship. Moreover, compared to pure LaAlO 3 , the synthesized LaAlO 3 -BiAlO 3 compound shows enhanced dielectric properties, which are promising in application as gate dielectric materials. (copyright 2013 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

O ingresso na universidade após os 45 anos: um evento não-normativo El ingreso a la universidad después de los 45 años: un evento no-normativo Admittance to the university after age 45: a non-normative event

Directory of Open Access Journals (Sweden)

Denise Maria dos Santos Paulinelli Raposo

2008-03-01

Full Text Available Examinam-se neste estudo as razões que levam à decisão de ingressar na universidade na idade adulta, um evento não dependente de gradação por idade, denominado pela perspectiva do curso de vida de evento não-normativo (non-normative event. Os 40 participantes (27 F e 13 M, alunos de uma universidade particular do Centro-Oeste, com idades entre 45 e 60 anos, responderam a seis questões de uma entrevista estruturada, cujas respostas foram preenchidas pela primeira autora. Os resultados indicaram que os respondentes vivenciaram na vida adulta um período de estabilidade e que, embora conscientes das expectativas do seu ambiente quanto ao tempo e à seqüência das transições do ciclo de vida, avaliaram seus recursos pessoais, selecionaram seus objetivos e otimizaram suas capacidades de reserva antes de ingressar no ensino superior. Os resultados são discutidos à luz da perspectiva do curso de vida.Se examinan en este estudio las razones que llevan a la decisión de ingresar a la universidad en la edad adulta, un acontecimiento no dependiente de gradación por edad, denominado por la perspectiva del curso de vida de evento no-normativo (non-normative event. Los 40 participantes (27 M y 13 H, alumnos de una universidad particular de la región centro-oeste brasileño, con edades entre 45 y 60 años, contestaron a seis preguntas de una encuesta estructurada cuyas respuestas fueron rellenadas por la primera autora. Los resultados indicaron que los respondientes vivenciaron en la vida adulta un periodo de estabilidad y que, aunque conscientes de las expectativas de su ambiente cuanto al tiempo y a la secuencia de las transiciones del ciclo de vida, evaluaron sus recursos personales, seleccionaron sus objetivos y optimizaron sus capacidades de reserva antes de ingresar en la enseñanza superior. Los resultados son discutidos a la luz de la perspectiva del curso de vida.The reasons for admittance to the university after age 45, a non

Structural and physical properties of the NaxCoO2·yH2O superconducting system

International Nuclear Information System (INIS)

Shi, Y G; Li, J Q; Yu, H C; Zhou, Y Q; Zhang, H R; Dong, C

2004-01-01

The structural features and physical properties of Na x CoO 2 and Na x CoO 2 ·yH 2 O materials have been investigated. The Na x CoO 2 -yH 2 O samples, in general, undergo superconducting transitions at around 3.5 K. Energy dispersive x-ray analyses suggest that our samples have average compositions of Na 0.65 CoO 2 for the parent compounds and Na 0.26 CoO 2 ·yH 2 O for the superconducting oxyhydrates. Transmission electron microscopy observations reveal a new superstructure with wave vector q = in the parent material. This superstructure becomes very weak in the superconducting samples. Electron energy loss spectra analyses show that the Co ions have valence states of around +3.3 in Na 0.65 CoO 2 and around +3.7 in Na 0.26 CoO 2 -yH 2 O

Degradation mechanism of alachlor during direct ozonation and O(3)/H(2)O(2) advanced oxidation process.

Science.gov (United States)

Qiang, Zhimin; Liu, Chao; Dong, Bingzhi; Zhang, Yalei

2010-01-01

The degradation of alachlor by direct ozonation and advanced oxidation process O(3)/H(2)O(2) was investigated in this study with focus on identification of degradation byproducts. The second-order reaction rate constant between ozone and alachlor was determined to be 2.5+/-0.1M(-1)s(-1) at pH 7.0 and 20 degrees C. Twelve and eight high-molecular-weight byproducts (with the benzene ring intact) from alachlor degradation were identified during direct ozonation and O(3)/H(2)O(2), respectively. The common degradation byproducts included N-(2,6-diethylphenyl)-methyleneamine, 8-ethyl-3,4-dihydro-quinoline, 8-ethyl-quinoline, 1-chloroacetyl-2-hydro-3-ketone-7-acetyl-indole, 2-chloro-2',6'-diacetyl-N-(methoxymethyl)acetanilide, 2-chloro-2'-acetyl-6'-ethyl-N-(methoxymethyl)-acetanilide, and two hydroxylated alachlor isomers. In direct ozonation, four more byproducts were also identified including 1-chloroacetyl-2,3-dihydro-7-ethyl-indole, 2-chloro-2',6'-ethyl-acetanilide, 2-chloro-2',6'-acetyl-acetanilide and 2-chloro-2'-ethyl-6'-acetyl-N-(methoxymethyl)-acetanilide. Degradation of alachlor by O(3) and O(3)/H(2)O(2) also led to the formation of low-molecular-weight byproducts including formic, acetic, propionic, monochloroacetic and oxalic acids as well as chloride ion (only detected in O(3)/H(2)O(2)). Nitrite and nitrate formation was negligible. Alachlor degradation occurred via oxidation of the arylethyl group, N-dealkylation, cyclization and cleavage of benzene ring. After O(3) or O(3)/H(2)O(2) treatment, the toxicity of alachlor solution examined by the Daphnia magna bioassay was slightly reduced. 2009 Elsevier Ltd. All rights reserved.

(μ-Acetato-κ2O:O′[μ-2,6-bis({bis[(pyridin-2-yl-κNmethyl]amino-κN}methyl-4-methylphenolato-κ2O:O](methanol-κOdizinc bis(perchlorate

Directory of Open Access Journals (Sweden)

Biswanath Das

2014-04-01

Full Text Available The binuclear title complex, [Zn2(C33H33N6O(CH3COO2(CH3OH](ClO42, was synthesized by the reaction between 2,6-bis({[bis(pyridin-2-ylmethyl]amino}methyl-4-methylphenol (H-BPMP, Zn(OAc2 and NaClO4. The two ZnII ions are bridged by the phenolate O atom of the octadentate ligand and the acetate group. An additional methanol ligand is terminally coordinated to one of the ZnII ions, rendering the whole structure unsymmetric. Other symmetric dizinc complexes of BPMP have been reported. However, to the best of our knowledge, the present structure, in which the two ZnII ions are distinguishable by the number of coordinating ligands and the coordination geometries (octahedral and square-pyramidal, is unique. The dizinc complex is a dication, and two perchlorate anions balance the charge. The –OH group of the coordinating methanol solvent molecule forms a hydrogen bond with a perchlorate counter-anion. One of the anions is disordered over two sets of sites with an occupancy ratio of 0.734 (2:0.266 (2.

Effects of Ca doping and O deficiency on the charge distribution in the vicinity of a 45° [001] grain boundary in YBa2Cu3O7

KAUST Repository

Schwingenschlögl, Udo

2012-02-01

The charge redistribution at grain boundaries is critical for the applicability of high-T c superconductors in electronic devices, because it determines the transport across the material. We investigate the charge transfer and the alterations of the electronic states due to local doping of a normal-state 45°-tilted [001] grain boundary in YBa 2Cu 3O 7 by means of first-principles calculations. Considering Ca doping and O deficiency as prototypical modifications we demonstrate that the redistribution of the charge carriers in the CuO 2 planes displays a very complex spatial pattern, which deviates even qualitatively from the naive expectation. Copyright © EPLA, 2012.

45 CFR 92.26 - Non-Federal audit.

Science.gov (United States)

2010-10-01

... fiscal year, shall: (1) Determine whether State or local subgrantees have met the audit requirements of... 45 Public Welfare 1 2010-10-01 2010-10-01 false Non-Federal audit. 92.26 Section 92.26 Public... Financial Administration § 92.26 Non-Federal audit. (a) Basic rule. Grantees and subgrantees are responsible...

A longitudinal study of Escherichia coli O157 in cattle of a Dutch dairy farm and in the farm environment

NARCIS (Netherlands)

Schouten, J.M.; Graat, E.A.M.; Frankena, K.; Giessen, van de A.W.; Zwaluw, van der W.K.; Jong, de M.C.M.

2005-01-01

From July 1999 till November 2000, a longitudinal study was conducted on a dairy farm in The Netherlands to study within herd prevalence and types of verocytotoxin producing Escherichia coli (VTEC) of serogroup O157 over time, and determine environmental reservoirs and possible transmission routes.

PREPARATION, CHARACTERIZATION, ACTIVITY, DEACTIVATION, AND REGENERATION TESTS OF CoO-MoO/ZnO AND CoO-MoO/ZnO-ACTIVATED ZEOLITE CATALYSTS FOR THE HYDROGEN PRODUCTION FROM FUSEL OIL

Directory of Open Access Journals (Sweden)

Wega Trisunaryanti

2010-06-01

Full Text Available Preparation, characterization, activation, deactivation, and regeneration tests of CoO-MoO/ZnO and CoO-MoO/ZnO-Activated Zeolite (AZ catalysts for the hydrogen production using steam reforming of alcohols in fusel oil have been conducted. Both catalysts were prepared by impregnation of Co and Mo onto ZnO or ZnO-AZ powder then followed by calcination at 400 °C for 5 h under N2 stream. The BET method and pyridine adsorption were used for catalysts characterization. The study of activation, deactivation, and regeneration of catalysts were conducted by using steam reforming method in the semi flow reactor. The reaction condition were: weight ratio of catalysts/feed = 0.1, temperature: 450 °C, duration: 45 min. The gas product was trapped in a 250 mL vacuum pyrex bottle filled with 50 mL of 4 M NaOH solution and analyzed by GC with TCD system to determine H2 existance and HCl titration to determine CO2 produced during the process that was dissolved in NaOH solution. The results showed that CoO-MoO/ZnO-AZ catalyst produced higher gas conversion than CoO-MoO/ZnO catalyst. However, it had short catalyst lifetime due to its high amount of coke deposited during the process. The regeneration test could enhance the catalyst activity. The gas product consisted of H2 (14.70% and CO2 (24.41%.   Keywords: fusel oil, steam reforming, deactivation, regeneration, hydrogen production.

Glass formation and properties of glasses in V/sub 2/O/sub 5/-B/sub 2/O/sub 3/-P/sub 2/O/sub 5/ system

Energy Technology Data Exchange (ETDEWEB)

Sedmale, G P; Vajvad, Ya A; Arkhipova, S E; Laukmanis, L A

1987-01-01

The glass formation in the system V/sub 2/O/sub 5/-B/sub 2/O/sub 3/-P/sub 2/O/sub 5/ and the properties of the obtained glasses have been studied by methods including that of the mathematical design and the treatment of the obtained data on ECM. The glass formation region is limited by the molar content of V/sub 2/O/sub 5/ 30-80%, B/sub 2/O/sub 3/ 0-45%, P/sub 2/O/sub 5/ 20-65%. The chemical stability data show that at the molar content of V/sub 2/O/sub 5/ 45-50% the transfer of vanadium from the state of the modificator to the glass-forming agent takes place. For the studied glasses the electron mechanism of conductivity is the dominating one.

Molecular Characterization of Human Atypical Sorbitol-Fermenting Enteropathogenic Escherichia coli O157 Reveals High Diversity.

Science.gov (United States)

Kossow, Annelene; Zhang, Wenlan; Bielaszewska, Martina; Rhode, Sophie; Hansen, Kevin; Fruth, Angelika; Rüter, Christian; Karch, Helge; Mellmann, Alexander

2016-05-01

Alongside the well-characterized enterohemorrhagic Escherichia coli (EHEC) O157:H7, serogroup O157 comprises sorbitol-fermenting typical and atypical enteropathogenic E. coli (EPEC/aEPEC) strains that carry the intimin-encoding gene eae but not Shiga toxin-encoding genes (stx). Since little is known about these pathogens, we characterized 30 clinical isolates from patients with hemolytic uremic syndrome (HUS) or uncomplicated diarrhea with respect to their flagellin gene (fliC) type and multilocus sequence type (MLST). Moreover, we applied whole-genome sequencing (WGS) to determine the phylogenetic relationship with other eae-positive EHEC serotypes and the composition of the rfbO157 region. fliC typing resulted in five fliC types (H7, H16, H34, H39, and H45). Isolates of each fliC type shared a unique ST. In comparison to the 42 HUS-associated E. coli (HUSEC) strains, only the stx-negative isolates with fliCH7 shared their ST with EHEC O157:H7/H(-) strains. With the exception of one O157:H(-) fliCH16 isolate, HUS was exclusively associated with fliCH7. WGS corroborated the separation of the fliCH7 isolates, which were closely related to the EHEC O157:H7/H(-) isolates, and the diverse group of isolates exhibiting different fliC types, indicating independent evolution of the different serotypes. This was also supported by the heterogeneity within the rfbO157 region that exhibited extensive recombinations. The genotypic subtypes and distribution of clinical symptoms suggested that the stx-negative O157 strains with fliCH7 were originally EHEC strains that lost stx The remaining isolates form a distinct and diverse group of atypical EPEC isolates that do not possess the full spectrum of virulence genes, underlining the importance of identifying the H antigen for clinical risk assessment. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Solar light-facilitated oxytetracycline removal from the aqueous phase utilizing a H2O2/ZnWO4/CaO catalytic system

Directory of Open Access Journals (Sweden)

Pankaj Raizada

2017-09-01

Full Text Available A CaO-supported ZnWO4 nanocomposite (ZnWO4/CaO was successfully synthesized using a novel hydrothermal method and was characterized by scanning electron microscopy (SEM, tunnelling electron microscopy (TEM, X-ray diffraction (XRD, electron diffraction X-ray (EDX, Fourier transform infrared spectroscopy (FTIR and UV–visible (UV–vis spectral analysis. The ZnWO4/CaO composites exhibited rod-like morphologies with variable lengths from 45 nm to 147 nm and diameters from 26 nm to 36 nm. The catalytic efficiency of the synthesized ZnWO4/CaO composites was displayed for the photodegradation of oxytetracycline (OTC antibiotic from the aqueous phase. The synergistic degradation of OTC was investigated in the presence of H2O2 and ZnWO4/CaO. The rate of photodegradation followed pseudo-first-order kinetics. The antibiotic removal was strongly influenced by the catalyst loading, H2O2 concentration, pH and OTC concentration. Using a solar/H2O2/ZnWO4/CaO catalytic system, 85% COD removal was attained for OTC degradation in 210 min. The oxidative degradation occurred through hydroxyl radicals. The prepared nanocomposites possessed high recyclability and were easily separated from the aqueous solution by a simple sedimentation process.

Cathodoluminescence properties of SiO2:Pr3+and ZnO.SiO2:Pr3+ phosphor nanopowders

CSIR Research Space (South Africa)

Mhlongo, GH

2010-10-01

Full Text Available regardless of the incorporation of Pr3+ and nanocrystalline ZnO or annealing at 600 °C. The particles were mostly spherical and agglomerated as confirmed by Field Emission Scanning Electron Microscopy. Thermogravimetric analysis of dried gels performed... Science, vol. 45(19): 5228-5236 Cathodoluminescence properties of SiO2:Pr 3+and ZnO·SiO2:Pr 3+ phosphor nanopowders G. H. Mhlongo, O. M. Ntwaeaborwa, M. S. Dhlamini, H. C. Swart, K. T. Hillie ABSTRACT: The successful incorporation of Zn...

Uma Análise da Aplicação de Três Métodos Estatísticos para o Cálculo do Desvio Padrão da Direção do Vento na Região Tropical

Directory of Open Access Journals (Sweden)

Adaiana Francisca Gomes da Silva

2016-03-01

Full Text Available Resumo Neste trabalho é apresentada uma revisão sobre métodos para cálculo do desvio padrão da direção do vento horizontal (σθ. Três diferentes métodos clássicos encontrados na literatura [Verral e Williams, 1982 (VW, Ackermann, 1983 (ACK, e Yamartino, 1984 (YM] são avaliados a partir de intercomparação entre eles utilizando-se dados de vento obtidos por radiossondagens. Estas medidas foram realizadas a cada seis horas durante um período de 11 dias do mês de setembro de 2008 (estação seca da região Norte do NE do Brasil, com ventos alísios intensos. Os cálculos mostraram que o método de YM apresentou-se o mais confiável, sendo que os resultados de VW foram muito próximos de YM. Comparando os métodos dois a dois, as máximas diferenças foram de 29 graus entre ACK e YM e 26,3 graus entre ACK e VW, enquanto que entre YM e VW a máxima diferença foi de apenas 2,6 graus. Segundo a literatura revisada, YM é o método mais estável ao longo da variação de σθ, com erro de, no máximo, 2%.

Microstructural dependence on relevant physical-mechanical properties on SiO2-Na2O-CaO-P2O5 biological glasses.

Science.gov (United States)

Rajendran, V; Begum, A Nishara; Azooz, M A; el Batal, F H

2002-11-01

Bioactive glasses of the system SiO2-Na2O-CaO-P2O5 have been prepared by the normal melting and annealing technique. The elastic moduli, attenuation, Vickers hardness, fracture toughness and fracture surface energy have been obtained using the known method at room temperature. The temperature dependence of elastic moduli and attenuation measurements have been extended over a wide range of temperature from 150 to 500 K. The SiO2 content dependence of velocities, attenuation, elastic moduli, and other parameters show an interesting observation at 45 wt% of SiO2 by exhibiting an anomalous behaviour. A linear relation is developed for Tg, which explores the influence of Na2O on SiO2-Na2O-CaO-P2O5 bioactive glasses. The measured hardness, fracture toughness and fracture surface energy show a linear relation with Young's modulus. It is also interesting to note that the observed results are functions of polymerisation and the number of non-bridging oxygens (NBO) prevailing in the network with change in SiO2 content. The temperature dependence of velocities, attenuation and elastic moduli show the existence of softening in the glass network structure as temperature increases.

Synthesis and crystal structure of a new 2,6-dimethyl piperazine-1,4-diium perchlorate monohydrate: [C{sub 6}H{sub 16}N{sub 2}](ClO{sub 4}){sub 2} · H{sub 2}O

Energy Technology Data Exchange (ETDEWEB)

Mleh, C. Ben [Université de Carthage, Laboratoire de Chimie des Matériaux, Faculté des Sciences de Bizerte (Tunisia); Roisnel, T. [Université de Rennes I, Centre de Diffractométrie X, UMR 6226 CNRS, Unité Sciences Chimiques de Rennes (France); Marouani, H., E-mail: houda.marouani@fsb.rnu.tn [Université de Carthage, Laboratoire de Chimie des Matériaux, Faculté des Sciences de Bizerte (Tunisia)

2017-03-15

A proton transfer compound 2,6-dimethyl piperazine-1,4-diium perchlorate monohydrate was synthesized by slow evaporation at room temperature using 2,6-dimethyl piperazine as template. The asymmetric unit contains one organic dication, two crystal graphically independent perchlorate anions and one water molecule. Each organic entities is engaged in a large number of bifurcated and non-bifurcated N–H···O (O) and C–H···O hydrogen bonds with different species and enhanced the three dimensional supramolecular network. In addition, the diprotonated piperazine ring adopts a chair conformation with the methyl groups occupying equatorial positions.

A meningococcal NOMV-FHbp vaccine for Africa elicits broader serum bactericidal antibody responses against serogroup B and non-B strains than a licensed serogroup B vaccine.

Science.gov (United States)

Pajon, Rolando; Lujan, Eduardo; Granoff, Dan M

2016-01-27

Meningococcal epidemics in Sub-Sahara caused by serogroup A strains are controlled by a group A polysaccharide conjugate vaccine. Strains with serogroups C, W and X continue to cause epidemics. Protein antigens in licensed serogroup B vaccines are shared among serogroup B and non-B strains. Compare serum bactericidal antibody responses elicited by an investigational native outer membrane vesicle vaccine with over-expressed Factor H binding protein (NOMV-FHbp) and a licensed serogroup B vaccine (MenB-4C) against African serogroup A, B, C, W and X strains. Human Factor H (FH) transgenic mice were immunized with NOMV-FHbp prepared from a mutant African meningococcal strain containing genetically attenuated endotoxin and a mutant sub-family B FHbp antigen with low FH binding, or with MenB-4C, which contains a recombinant sub-family B FHbp antigen that binds human FH, and three other antigens, NHba, NadA and PorA P1.4, capable of eliciting bactericidal antibody. The NOMV-FHbp elicited serum bactericidal activity against 12 of 13 serogroup A, B, W or X strains from Africa, and four isogenic serogroup B mutants with sub-family B FHbp sequence variants. There was no activity against a serogroup B mutant with sub-family A FHbp, or two serogroup C isolates from a recent outbreak in Northern Nigeria, which were mismatched for both PorA and sub-family of the FHbp vaccine antigen. For MenB-4C, NHba was expressed by all 16 African isolates tested, FHbp sub-family B in 13, and NadA in five. However, MenB-4C elicited titers ≥ 1:10 against only one isolate, and against only two of four serogroup B mutant strains with sub-family B FHbp sequence variants. NOMV-FHbp has greater potential to confer serogroup-independent protection in Africa than the licensed MenB-4C vaccine. However, the NOMV-FHbp vaccine will require inclusion of sub-family A FHbp for coverage against recent serogroup C strains causing outbreaks in Northern Nigeria. Copyright © 2015 Elsevier Ltd. All rights

Verocytotoxin-producing Escherichia coli O26 in raw water buffalo (Bubalus bubalis) milk products in Italy.

Science.gov (United States)

Lorusso, Vanessa; Dambrosio, Angela; Quaglia, Nicoletta Cristiana; Parisi, Antonio; La Salandra, Giovanna; Lucifora, Giuseppe; Mula, Giuseppina; Virgilio, Sebastiano; Carosielli, Leonardo; Rella, Addolorata; Dario, Marco; Normanno, Giovanni

2009-08-01

Escherichia coli 026 is known as a verocytotoxin-producing E. coli (VTEC) organism that causes severe foodborne diseases such as hemorrhagic colitis and hemolytic uremic syndrome. Although cattle are the most important reservoir of VTEC, only a few reports on the role of water buffalo (Bubalus bubalis) as a reservoir of VTEC and on the presence of these organisms in their milk are available. However, in Southern Italy, where water buffalo are intensively reared, an outbreak of hemolytic uremic syndrome due to E. coli 026 has recently been reported, in which the consumption of typical dairy products was considered to be a common risk factor. The aims of this work were to assess the prevalence of E. coli O26 in raw water buffalo milk, to characterize the virulence gene profiles of the isolates, and to evaluate their phenotypic antimicrobial resistance pattern. Of 160 analyzed samples, 1 (0.6%) tested positive for E. coli O26, and the isolate showed the stx1+/stx2+/eae-/hlyA+ genotypic profile. The strain showed resistance against glycopeptides, macrolides, and penicillins. The presence of VTEC organisms in raw water buffalo milk could be considered to be a potential threat to consumers; however, the strict adherence to the processes used in the preparation of the most common buffalo dairy products could strongly mitigate the foodborne risk. To our knowledge, this article reports the first isolation and characterization of E. coli O26 VTEC in raw water buffalo milk.

Factor analysis of serogroups botanica and aurisina of Leptospira biflexa.

Science.gov (United States)

Cinco, M

1977-11-01

Factor analysis is performed on serovars of Botanica and Aurisina serogroup of Leptospira biflexa. The results show the arrangement of main factors serovar and serogroup specific, as well as the antigens common with serovars of heterologous serogroups.

ZnO-PbO-B2O3 glasses as gamma-ray shielding materials

International Nuclear Information System (INIS)

Singh, Harvinder; Singh, Kulwant; Gerward, Leif; Singh, Kanwarjit; Sahota, Hari Singh; Nathuram, Rohila

2003-01-01

Values of the gamma-ray mass-attenuation coefficient, the photon mean free path (MFP), the effective atomic number and the effective electron density have been determined experimentally for xZnO · 2xPbO · (1-3x)B 2 O 3 (x=0.1-0.26) glasses at photon energies 511, 662, 1173 and 1332 keV and compared with theoretical data. The specific volume of the glasses has been derived from density measurements and studied as a function of composition. It is pointed out that these glasses have potential applications in radiation shielding

Two new hybrid molybdenum arsenate derivative constructed from [As2Mo6O26]6- building: Synthesis, structural characterization and photocatalysis property

Science.gov (United States)

Zhang, Xiao; Luo, Xuan; Duan, Yuanling; Huang, Yanping; Zhang, Nanxi; Zhao, Liyan; Wu, Jie

2017-08-01

Two new inorganic-organic hybrid materials [Cu(enMe)2]2{(As2Mo6O26) [Cu(enMe)2]}·4H2O (1) and [As2Mo6(OH)2O24][Cu(H2O)2(phen)]2 (2) (enMe = 1,2'-propanediamine, phen = 1,10'-phenanthroline) based on [As2Mo6O26]6- building blocks, denoted as [As2Mo6], have been obtained by hydrothermal methods. 1 shows a 1-D straight chain structure constructed form [As2Mo6] building blocks and [Cu(enMe)2] complexes, and then extended to 3-D supramolecular network by lattice water via hydrogen bonds interactions. 2 exhibits a new 1-D covalent ribbon with large rectangular grids formed from [As2Mo6] building blocks connected by [Cu(H2O)2(phen)] complexes, then extended into 3-D supramolecular network via hydrogen bonds and π···π interactions. In additional, the photocatalytic activity for methylene blue degradation under visible-light irradiation of 2 was investigated.

Study on Dy0.45Ba0.05Sr0.5Co0.8Fe0.2O3-δ-Ce0.85Gd0.15O1.95 composite cathode material for intermediate temperature solid oxide fuel cell

Science.gov (United States)

Kautkar, Pranay R.; Acharya, Smita A.

2018-05-01

xDy0.45Ba0.05Sr0.5Co0.8Fe0.2O3-δ - xCe0.85Gd0.15O1.95 (x = 50 %) composite cathode supported on Ce0.85Gd0.15O1.95 (GDC15) electrolyte are studied for applications in IT-SOFCs. Results attribute that Dy0.45Ba0.05Sr0.5Co0.8Fe0.2O3-δ material is chemically compatible with Ce0.85Gd0.15O1.95 (GDC15). Rietveld refined X-ray diffraction patterns notify orthorhombic (space group:Pbnm) symmetry for Dy0.45 Ba0.05Sr0.5Co0.8Fe0.2O3-δ and fluorite type structure (space group: Fm-3m) symmetry for GDC15. The polarization resistance (Rp) of composite cathode reduces to the minimum value of 1.35 Ω cm2 at 650 °C in air. Area specific resistance (ASR) of composite cathode has found 0.67 Ω.cm2 at 650°C respectively. Result shows that the surface diffusion of the dissociative adsorbed oxygen at electrode/electrolyte interface on the composite cathode.

Comparação e correlação entre automedida, medida casual e monitorização ambulatorial da pressão arterial

Directory of Open Access Journals (Sweden)

Weimar Kunz Sebba Barroso de Souza

2011-08-01

Full Text Available FUNDAMENTO: A medida casual da pressão arterial (PA pelos profissionais de saúde está sujeita a uma grande variabilidade, sendo necessário buscar novos métodos que possam superar essa limitação. OBJETIVO: Comparar e avaliar a correlação entre os níveis de PA obtidos por meio da automedida da pressão arterial (AMPA com a medida casual e com a monitorização ambulatorial da pressão arterial (MAPA. MÉTODOS: Avaliamos hipertensos que realizaram as três metodologias de medida da PA com intervalo menor que 30 dias; as médias das pressões foram utilizadas para comparação e correlação. Foram empregados os aparelhos: OMRON 705 CP (medida casual, OMRON HEM 714 (AMPA e SPACELABS 9002 (MAPA. RESULTADOS: Foram avaliados 32 pacientes, 50,09% mulheres, idade média 59,7 (± 11,2 anos, média do IMC 26,04 (± 3,3 kg/m². Valores médios de pressão sistólica (PAS e pressão diastólica (PAD para a AMPA foram de 134 (± 15,71mmHg e 79,32 (± 12,38 mmHg. Na medida casual as médias da PAS e PAD foram, respectivamente, 140,84 (± 16,15mmHg e 85 (± 9,68 mmHg. Os valores médios da MAPA na vigília foram 130,47 (± 13,26 mmHg e 79,84 (± 9,82 mmHg para PAS e PAD, respectivamente. Na análise comparativa, a AMPA apresentou valores semelhantes aos da MAPA (p > 0,05 e diferentes da medida casual (p < 0,05. Na análise de correlação a AMPA foi superior à medida casual, considerando a MAPA como o padrão de referência nas medidas tensionais. CONCLUSÃO: A AMPA apresentou melhor comparação com a MAPA do que a medida casual e também se correlacionou melhor com a aquela, especialmente para a pressão diastólica, devendo ser considerada uma alternativa com baixo custo para o acompanhamento do paciente hipertenso.

Engajamento escolar: explicação a partir dos valores humanos

Directory of Open Access Journals (Sweden)

Patrícia Nunes da Fonsêca

Full Text Available Resumo O presente estudo objetivou conhecer em que medidas os valores humanos predizem o engajamento escolar. Participaram 338 estudantes, com idade média de 13,04 (DP=1,45, sendo a maioria do sexo masculino (52,4%, provenientes de escolas públicas (53,6% e do nono ano do ensino fundamental (26,3%. Estes responderam à Escala de Engajamento Escolar, o Questionário de Valores Básicos e perguntas demográficas. Utilizou-se o software estatístico SPSS para a realização de análises descritivas e inferenciais. Os resultados indicaram que os alunos que priorizam valores das subfunções interativa, normativa, suprapessoal, existência e realização dedicam-se na realização da tarefa acadêmica, ao contrário daqueles que priorizam os valores da subfunção experimentação. Ademais, constatou-se que as variáveis nível de escolaridade e natureza da instituição escolar influenciaram no engajamento escolar. Os resultados evidenciam a importância dos valores em ambiente acadêmico, mostrando a necessidade do desenvolvimento de projetos que possam estimulá-los nas escolas, sobretudo àqueles de orientação central e social.

Virulence factors of non-O1 non-O139 Vibrio cholerae isolated in Córdoba, Argentina Factores de virulencia de Vibrio cholerae no-O1 no-O139 aislados en Córdoba, Argentina

Directory of Open Access Journals (Sweden)

C. Bidinost

2004-12-01

Full Text Available V. cholerae non-O1 non-O139 serogroups isolated from clinical and environmental sources in Córdoba, Argentina, were analyzed for the presence and expression of virulence genes. Most of the strains studied contained the genes toxR and hlyA, but lacked ctxA, zot, ace, tcpA and stn. The culture supernatants were tested for hemolytic and cytotoxic activity. The enterotoxic potential of the strains was studied in a rabbit ileal loop assay and their genetic profiles were compared by PFGE. The environmental strains varied in their virulence phenotype and showed no-clonal relationships. The clinical strains were highly enterotoxic, hemolytic, proteolytic and showed indistinguishable PFGE profiles, although they differed in their cytotoxic activity. This is the first description, using cell culture and “in vivo” studies, of the virulence properties of non-O1 non-O139 V. cholerae from Argentina.En este trabajo se analizó la presencia y expresión de genes de virulencia en V. cholerae no-O1 no-O139 de origen clínico y ambiental, aislados en Córdoba, Argentina. La mayoría de las cepas estudiadas contiene los genes toxR y hlyA, pero no ctxA, zot, ace, tcpA y stn. Se analizó la actividad hemolítica y citotóxica de estas cepas en los sobrenadantes de cultivo, así como su potencial enterotóxico en ensayos de asa ileal ligada de conejo. Además, los aislamientos fueron comparados por sus perfiles genéticos en PFGE. Las cepas del medio ambiente mostraron variación en su fenotipo de virulencia y no mostraron relación clonal. Las cepas clínicas fueron muy enterotóxicas, hemolíticas, proteolíticas y mostraron perfiles indistinguibles de PFGE, aunque mostraron diferencias en su actividad citotóxica. En este trabajo se describen por primera vez, utilizando ensayos de cultivo celular e “in vivo”, propiedades de virulencia de V. cholerae no-O1 no-O139 aislados en Argentina.

In-vitro study of copper doped SiO2-CaO-P2O5 system for bioactivity and antimicrobial properties

Science.gov (United States)

Kaur, Kulwinder; Singh, K. J.; Anand, Vikas; Kaur, Harpreet; Arora, Daljit Singh

2015-08-01

Samples of the xCuO-(45-x)CaO-10P2O5-45SiO2 system (x varies from 0 to 4 mole%) have been synthesized for application as biomaterials to slow or inhibit the growth of living organisms (fungi and other pathogenic microorganisms) by the combination of sol-gel and co-precipitation processes. Prepared samples have been characterized by X-Ray Diffraction, Fourier Transform Infra-Red and Field Emission Scanning Electron Microscopy techniques before and after immersion in simulated body fluid. Antimicrobial activity of samples has been investigated against Staphylococcus aureus. Releasing of Cu2+and other ions in the simulated body fluid has been determined by Atomic Absorption Spectroscopy to ensure the use of prepared material as biomaterial with good antibacterial properties.

Sincronização do estro, indução da ovulação e fertilidade de ovelhas deslanadas após tratamento hormonal com gonadotrofina coriônica eqüina

Directory of Open Access Journals (Sweden)

Dias F.E.F.

2001-01-01

Full Text Available Utilizou-se a sincronização do estro com esponjas vaginais de 30mg de acetato de fluorogestona durante 12 dias para avaliar o efeito de diferentes doses de eCG sobre o desempenho reprodutivo de ovelhas. Na retirada das esponjas as ovelhas foram divididas em três grupos para receberem 0 (n=26, 200 (n=30 ou 400UI (n=30 de eCG. O estro foi detectado a cada 12h utilizando-se um rufião. As fêmeas foram inseminadas por laparoscopia 60h após a retirada das esponjas. Realizaram-se colheitas de sangue aos 5 e 18 dias pós-inseminação para dosagem de progesterona e determinação do número de ovulações e prenhezes, respectivamente. A fertilidade foi verificada por ecografia aos 60 dias e ao parto. Das 86 ovelhas 70,9% apresentaram estro. Essa porcentagem foi maior (P<0,05 nas fêmeas tratadas com eCG: 96,7% (400UI e 76,7% (200UI versus 34,6% (0 UI. O intervalo entre o final do tratamento e o início do estro foi maior (P<0,05 no grupo sem eCG, 54,7±6,3h versus 45,9±7,8h para 200UI e 40,4±10,3h para 400UI. Verificou-se menor (P<0,05 número de ovulações e prenhezes no grupo sem eCG. A não aplicação de eCG influiu negativamente no desempenho reprodutivo de ovelhas deslanadas.

Disorder influence on the magnetic properties of La{sub 0.55}Sr{sub 0.45}MnO{sub 3}/SrTiO{sub 3} superlattices

Energy Technology Data Exchange (ETDEWEB)

Haberkorn, N. E-mail: nhaberk@cab.cnea.gov.ar; Sirena, M.; Guimpel, J.; Steren, L.B

2004-05-01

The structural and physical properties of La{sub 0.55}Sr{sub 0.45}MnO{sub 3}/SrTiO{sub 3} superlattices grown by magnetron sputtering are studied. Two deposition temperatures and different mismatched substrates and buffer layers were used. The structure was determined by refinement through X-ray diffraction pattern fitting. The results indicate 1 unit cell (u.c.) interdiffusion at the interfaces and a 1 u.c. layer thickness fluctuation, i.e. roughness. In-plane hysteresis loops show the expected ferromagnetic behavior, while the perpendicular-to-plane hysteresis loops show anomalies which could evidence magnetic domain size inhomogeneities and stress.

Crystal structure of LaFe5Ge3O15 = LaFe5[GeO4][Ge2O7]O4

International Nuclear Information System (INIS)

Genkina, E.A.; Maksimov, B.A.; Mill, B.V.

1991-01-01

The authors have determined the structure of a new lanthanum-iron germanate LaFe 5 [GeO 4 ][GeO 4 ][Ge 2 O 7 ]O 4 (a = 18.040(4), b = 17.012(4), c = 7.591(1) angstrom, V = 2330.2(9) angstrom 3 , Z = 8, ρ t = 4.99 g/cm 3 , space ground Cmca, 1976 I hkl ≥ 3 σ(I), R = 4.5%). The compound is interesting because the framework simultaneously contains ortho- and diorthogroups of Ge and because of a classical set of coordination numbers (4,5,6) characteristic of trivalent iron within the composition of one structure. The coordination polyhedron of La has nine vertices

Análise da cadeia de produção de verduras em Ribeirão Preto, SP Analysis of the vegele productive chain in Ribeirão Preto, SP

Directory of Open Access Journals (Sweden)

Osvaldo M. Takayanagui

2006-04-01

Full Text Available Com o objetivo de avaliar o risco cumulativo de contaminação de hortaliças, investigou-se 45 cadeias produtoras. Foi detectada a presença de coliformes a 45ºC, Salmonella e/ou parasitas em 69% delas, em todas as etapas de produção. Ressalta-se a necessidade de se intensificar o controle de qualidade ao longo da cadeia produtiva.With the aim of assessing the cumulative risk of lettuce contamination, 45 production chains were investigated. The presence of thermotolerant coliforms, Salmonella and/or parasites was detected in 69% of these, in all steps of the production chain. Quality control in all steps of the lettuce production process should be intensified.

[Cytotoxic effect of Vibrio cholerae non-O1 on Vero cells].

Science.gov (United States)

Figueroa-Arredondo, P; García-Lozano, H; Gutiérrez-Cogco, L; Valdespino-Gómez, J L

1994-01-01

At the present time there is still in Mexico a diarrhoeal outbreak due to Vibrio cholerae O1. In INDRE we have isolated from the same outbreak last year (jan-apr), 70 strains of Vibrio cholerae Non-O1. These were isolated from patients with a diarrhoeal illness different from cholera. Patients were of different ages and sex, and from various geographic areas. The isolated strains were confirmed by serological agglutination test with polyclonal antisera, and they neither belong to O1 serogroup or O139. We assayed all the 70 strains in Vero cells, searching for cytotoxic effect, probably attributed to cholera toxin, or any other toxin. The strains were screened by PCR for cholera toxin gene detection, and negative results were obtained. We have found only one CT-producer strain, but it was a rough one so, we are not able to affirm that is not a V. cholerae O1 serotype. Vibrio cholerae Non-O1 strains, tested in Vero cells assay, produced cytotoxic effect within 24 h. It was found that 48/70 strains (66.6%), had cytotoxic activity, showing rounding and then lysis of cells. From our results we concluded that this cytotoxic effect, is not cholera toxin related, instead we propose it could be due to an unknown virulence factor, probably a different toxin in mexican Vibrio cholerae Non-O1 strains.

Scintillation and optical properties of TiO2-ZnO-Al2O3-B2O3 glasses and glass-ceramics

Science.gov (United States)

Usui, Yuki; Okada, Go; Kawaguchi, Noriaki; Masai, Hirokazu; Yanagida, Takayuki

2018-04-01

13TiO2-xZnO-17Al2O3-(70 - x)B2O3 (x = 17, 26, and 35) glasses were prepared by a melt-quenching method, and the obtained glass samples were heated at temperatures 30 °C above the glass transition temperature of corresponding glass in order to obtain glass-ceramics. The obtained glass-ceramic samples were confirmed to have anatase (x = 17) and rutile (x = 26 and 35) phases from X-ray diffraction analysis. Then, the scintillation and optical properties were evaluated and discussed the difference between the glass-ceramic and glass samples. In the scintillation spectra under X-ray irradiation, a broad emission peak was observed around 450 nm in all the samples, and the new peak around 500 nm appeared in the anatase-precipitated glass-ceramic. The intensities of the glass-ceramic samples were enhanced in comparison with the corresponding glasses because the glass-ceramics includes TiO2 crystallites with defect centers which act as effective emission centers. The scintillation decay curves of the glass and glass-ceramic samples were approximated by one and a sum of two exponential decay functions, respectively. The faster component of glass and glass-ceramic samples would be caused by the host emission, and the slower component of glass-ceramic sample would be ascribed to the emission of Ti3+.

Ab-initio study of the interfacial properties in ultrathin MgO films on O-rich FeO/Fe(001) surfaces

Energy Technology Data Exchange (ETDEWEB)

Jeon, Junjin; Yu, Byungdeok [University of Seoul, Seoul (Korea, Republic of)

2014-09-15

Using ab-initio simulations based on density functional theory, we systematically studied the interfacial properties of MgO films on O-rich FeO/Fe(001) surfaces with increasing number of MgO layers from one to three monolayers (MLs). The structural and the adhesion properties of the MgO/FeO/Fe(001) system were assessed and compared with those of simple MgO/Fe(001) interfaces. Our calculated results showed that the adhesion energy for MgO/FeO/Fe(001) was smaller than that for simple MgO/Fe(001). An analysis of the electronic structures and the charge rearrangements of the MgO/FeO/Fe(001) interfaces was also performed. The work functions of the MgO/FeO/Fe(001) systems upon the deposition of MgO films exhibited smaller decreases (0.45 - 0.67 eV) than those (1.43 - 1.74 eV) of the MgO/Fe(001) systems. In addition, the obtained work functions (3.77 - 3.99 eV) for MgO/FeO/Fe(001) were much larger than those (2.12 - 2.43 eV) for MgO/Fe(001).

Rapid and simple method by combining FTA™ card DNA extraction with two set multiplex PCR for simultaneous detection of non-O157 Shiga toxin-producing Escherichia coli strains and virulence genes in food samples.

Science.gov (United States)

Kim, S A; Park, S H; Lee, S I; Ricke, S C

2017-12-01

The aim of this research was to optimize two multiplex polymerase chain reaction (PCR) assays that could simultaneously detect six non-O157 Shiga toxin-producing Escherichia coli (STEC) as well as the three virulence genes. We also investigated the potential of combining the FTA™ card-based DNA extraction with the multiplex PCR assays. Two multiplex PCR assays were optimized using six primer pairs for each non-O157 STEC serogroup and three primer pairs for virulence genes respectively. Each STEC strain specific primer pair only amplified 155, 238, 321, 438, 587 and 750 bp product for O26, O45, O103, O111, O121 and O145 respectively. Three virulence genes were successfully multiplexed: 375 bp for eae, 655 bp for stx1 and 477 bp for stx2. When two multiplex PCR assays were validated with ground beef samples, distinctive bands were also successfully produced. Since the two multiplex PCR examined here can be conducted under the same PCR conditions, the six non-O157 STEC and their virulence genes could be concurrently detected with one run on the thermocycler. In addition, all bands clearly appeared to be amplified by FTA card DNA extraction in the multiplex PCR assay from the ground beef sample, suggesting that an FTA card could be a viable sampling approach for rapid and simple DNA extraction to reduce time and labour and therefore may have practical use for the food industry. Two multiplex polymerase chain reaction (PCR) assays were optimized for discrimination of six non-O157 Shiga toxin-producing Escherichia coli (STEC) and identification of their major virulence genes within a single reaction, simultaneously. This study also determined the successful ability of the FTA™ card as an alternative to commercial DNA extraction method for conducting multiplex STEC PCR assays. The FTA™ card combined with multiplex PCR holds promise for the food industry by offering a simple and rapid DNA sample method for reducing time, cost and labour for detection of STEC in

Photocatalytic degradation of Acephate, Omethoate, and Methyl parathion by Fe_3O_4@SiO_2@mTiO_2 nanomicrospheres

International Nuclear Information System (INIS)

Zheng, Lingling; Pi, Fuwei; Wang, Yifan; Xu, Hui; Zhang, Yinzhi; Sun, Xiulan

2016-01-01

Highlights: • An efficient photocatalyst Fe_3O_4@SiO_2@mTiO_2 with high magnetic response and large specific surface area was synthesized. • Photocatalytic efficiency of Fe_3O_4@SiO_2@mTiO_2 on Acephate, Omethoate, and Methyl Parathion was higher than TiO_2 P-25. • Possible photocatalytic degradation mechanisms for the Acephate, Omethoate, and Methyl Parathion were proposed. - Abstract: A novel magnetic mesoporous nanomicrospheres Fe_3O_4@SiO_2@mTiO_2 were synthetized and characterized by a series of techniques including FE-TEM, EDS, FE-SEM, PXRD, XPS, BET, TGA as well as VSM, and subsequently tested as a photocatalyst for the degradation of Acephate, Omethoate, and Methyl parathion under UV irradiation. The well-designed nanomicrospheres exhibit a pure and highly crystalline anatase TiO_2 layer, large specific surface area, and high-magnetic-response. Photocatalytic degradation of the three organophosphorus pesticides (OPPs) and the formation intermediates were identified using HPLC, TOC-V_c_p_n, IC, pH meter and GC–MS. Acephate, Omethoate, and Methyl parathion disappeared after 45 min, 45 min, and 80 min UV illumination, respectively. At the end of the treatment, the total organic carbon (TOC) of the OPPs was reduced 80–85%. The main mineralization products were SO_4"2"−, NO_3"− and PO_4"3"− and Omethoate additionally formed NO_2"−. Based on the results, we proposed the photocatalytic degradation pathways for Acephate, Omethoate, and Methyl parathion.

Testes de deterioração controlada e envelhecimento acelerado para avaliação do vigor em nabo forrageiro

Directory of Open Access Journals (Sweden)

Camila Santos Barros de Morais

Full Text Available O objetivo do trabalho foi verificar a eficiência de diferentes procedimentos para condução dos testes de envelhecimento acelerado e deterioração controlada visando avaliação da qualidade fisiológica de sementes de nabo forrageiro (Raphanus Sativus L. var oleiferus. Quatro lotes de sementes de nabo forrageiro da cultivar CATI AL 1000 foram caracterizados inicialmente pelos testes de germinação, primeira contagem, comprimento e massa de plântulas, condutividade elétrica, emergência em areia e campo e índice de velocidade de emergência em areia e campo. Em seguida, foram submetidos aos testes envelhecimento acelerado com ou sem solução saturada de NaCl sob temperatura de 42 e 45 ºC durante 36 e 48 horas, e aos testes de deterioração controlada com sementes pré-umedecidas a 15; 20 e 25% sob temperatura de 42 e 45 ºC durante 36 e 48 horas. O teste de envelhecimento acelerado (com solução de NaCl sob temperatura de 45 ºC durante 36 horas e o de deterioração controlada (com sementes pré-umedecidas a 20% sob temperatura de 45 ºC durante 36 horas, assim como o de emergência de plântulas em campo, foram eficientes na classificação dos lotes de sementes de nabo forrageiro em quatro distintos níveis de vigor.

Análise espaço temporal da evapotranspiração na área de preservação ambiental da Ilha de Santa Rita, Alagoas, Brasil

Directory of Open Access Journals (Sweden)

Admilson Penha Pacheco

2014-06-01

Full Text Available A evapotranspiração (ET é uma das principais componentes do ciclo hidrológico. Nesse contexto, este trabalho teve como objetivo analisar o comportamento espaço-temporal do Balanço de Energia e da Evapotranspiração Real Diária na Área de Preservação Ambiental da Ilha de Santa Rita, Alagoas, Brasil, tomando como subsídio as possíveis alterações no uso e cobertura do solo. A metodologia utilizada envolve o uso do algoritmo SEBAL, imagens do satélite Landsat 5 - TM e dados complementares de estação meteorológica localizada na área de estudo. A estimativa diária de ET24h real variou de 0 a 1 mm dia-1 no solo exposto e na cidade; a 6 mm dia-1, em corpos d'água e mangue, com um valor médio de 3,78 mm dia-1, 3,45 mm dia-1 e 4,75 mm dia-1, nos dias 03/09/2003, 26/08/2006 e 17/03/2011, respectivamente, em toda a área estudada. Os resultados por meio do algoritmo SEBAL (SURFACE ENERGY BALANCE ALGORITHMS FOR LAND podem ser utilizados em condições climáticas semelhantes às da Área de Preservação Ilha de Santa Rita/Alagoas. As distribuições de cada componente do balanço de energia: saldo de radiação, fluxo de calor no solo, sensível e latente, apresentaram influência significativa nos diferentes tipos de uso e cobertura do solo. Além disso, constataram-se diferenças evidentes entre os fluxos em zona rural e área urbana. A abordagem utilizada é muito adequada para uma exploração real de dados de satélite, podendo ser útil para estimar um número de parâmetros no contínuo solo-planta-atmosfera.

Produtividade de cultivares de alho na região paulista de Tietê Garlic cultivars yield in Tietê region, São Paulo State, Brazil

Directory of Open Access Journals (Sweden)

Paulo Espíndola Trani

2008-01-01

Full Text Available O custo elevado das sementes (bulbilhos de alho nobre e a vernalização podem aumentar a produtividade da cultura, mas dificultam seu cultivo por pequenos produtores, responsáveis pela maior parte da produção no Brasil. Assim, estudos de seleção de sementes de menor custo como os alhos comuns, feitos regionalmente, e de comparação com alho vernalizado quanto à produtividade, são fundamentais. O objetivo deste trabalho foi a avaliação da produtividade de quatro cultivares de alho comum não vernalizado (Assaí, Gigante de Curitibanos, Santa Catarina Branco e Santa Catarina Roxo, em comparação com alho chinês vernalizado Jonas (armazenamento dos bulbos a 4 ºC por 40 dias. O delineamento experimental foi em blocos casualizados com cinco repetições. O experimento foi realizado em área experimental da APTA-UPD de Tietê (SP, de março a setembro de 2005. A variedade mais produtiva foi Jonas, sem diferir de Santa Catarina Roxo, Santa Catarina Branco e Assaí 3702, com 8,4; 7,6; 7,4 e 6,9 t ha-1 respectivamente e também com as maiores massas médias de bulbos (30,5; 27,0; 26,3 e 24,6g respectivamente. Quanto ao diâmetro do bulbo, os alhos comuns, exceto Gigante de Curitibanos, produziram bulbos tamanho 4 (36-45 mm. Para produtividade e massa do bulbo, a cultivar Gigante de Curitibanos foi inferior a Jonas.The high cost of garlic seed and the refrigeration process may increase yield, but it is a barrier for small farmers who are responsible for the large Brazilian production. Therefore, research seeking low cost garlic seed, regionally made, and competition studies for yield between refrigerated and no refrigerated cultivars are fundamental. This work aimed to evaluate yield and bulb quality of four no refrigerated garlic varieties (Assaí 3702, Gigante de Curitibanos, Santa Catarina Branco and Santa Catarina Roxo and the refrigerated type Jonas (storage at 4º C for 40 days. The field trial was carried out during March

Atração e distração na publicidade externa dos candidatos à eleição em 2014

Directory of Open Access Journals (Sweden)

Sérgio Trein

2015-12-01

CÉSAR, Newton. Direção de arte em propaganda. São Paulo: Futura, 2004. COLLARO, Antonio Celso. Projeto Gráfico Teoria e Prática da Diagramação. São Paulo: Summus Editorial, 1987.  DONDIS, Donis A.. Sintaxe da linguagem visual. 2. ed. São Paulo: Martins Fontes, 2003.  HILLMAN, James. Cidade & alma. São Paulo: Studio Nobel, 1993. HURLBURT, Allen. Layout o design da página impressa. São Paulo: Nobel, 2002. MOLES, Abraham Antoine. O cartaz. São Paulo: Perspectiva, 1974. MÜLLER, Josef Brockmann. History of visual communication. Teufen: Nigli, 1971. PÈNINOU, Georges. Semiótica de la publicidad. Barcelona: Gustavo Gili, 1976.   Site consultado: Outdoor.com. Acessado em: 26 de março de 2015.   Disponível em:  Url: http://opendepot.org/2703/   Abrir em (para melhor visualização em dispositivos móveis - Formato Flipbooks: Issuu / Calameo

45 CFR 1157.26 - Non-Federal audit.

Science.gov (United States)

2010-10-01

... 45 Public Welfare 3 2010-10-01 2010-10-01 false Non-Federal audit. 1157.26 Section 1157.26 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS AND THE...-Federal audit. (a) Basic rule. Grantees and subgrantees are responsible for obtaining audits in accordance...

O que é Ciência da Informação?

Directory of Open Access Journals (Sweden)

Carlos Alberto Ávila Araújo

2014-12-01

Full Text Available Introdução: Apresenta um panorama da Ciência da Informação em três momentos. Inicialmente, seu surgimento e consolidação na década de 1960, como confluência de vários fatos: a distinção em relação à Arquivologia, à Biblioteconomia e à Museologia; a relação com a Documentação; a ocupação do espaço institucional da Biblioteconomia; as atividades dos primeiros “cientistas da informação”; as tecnologias da informação; e o uso da Teoria Matemática. Objetivo: Analisar a ampliação vivida nas décadas seguintes com o desenvolvimento de subáreas, das caracterizações do campo e da evolução do conceito de informação. Metodologia: Revisão de Literatura. Resultados: São apresentadas as tendências contemporâneas: as sistematizações da área, os três modelos para o estudo da informação e o desafio recente de diálogo com a Arquivologia, a Biblioteconomia e a Museologia. Conclusões: muitos processos e eventos aconteceram no campo da Ciência da Informação nestes últimos 45 anos. Diferentes subáreas foram formadas, conceitos formulados e reformulados, caracterizações problematizadas e discutidas, relações com outros campos de conhecimento tensionadas e concretizadas.

Características clínicas e citogenéticas da síndrome de Turner na região Centro-Oeste do Brasil Clinical and cytogenetic aspects of the Turner syndrome in the Brazilian Western region

Directory of Open Access Journals (Sweden)

Claudinéia de Araújo

2010-08-01

Full Text Available OBJETIVO: examinar a associação entre características citogenéticas e alterações clínicas em pacientes com síndrome de Turner (ST. MÉTODOS: Foram incluídas 42 pacientes. Os dados clínicos foram colhidos e registrados em formulário padronizado com entrevista do responsável e, quando possível, com a própria paciente, seguido de exame físico detalhado. A associação entre cariótipo e intercorrências clínicas foram examinadas pelo teste do χ2. RESULTADOS: Das pacientes, 64% tinham cariótipo 45,X; 26,2% 45,X/46,X; 7% 45,X/46Xi(Xq e 2,3% 45,X/46,X,Del(Xq. Independentemente do cariótipo, 100% das pacientes apresentaram baixa estatura. A implantação baixa dos cabelos foi mais frequente nas pacientes com cariótipo 45,X (p=0,03. Anomalias cardiovasculares (45%, otites (43%, disfunção da tireoide (33% e hipertensão arterial (26,6% foram as alterações clínicas mais frequentes e não mostraram correlação com o cariótipo. A avaliação de medidas antropométricas revelou correlação positiva entre a idade e o diâmetro da cintura e quadril (r=0,9; p=0,01. Trinta e uma pacientes (74% faziam ou tinham feito uso de medicamentos, sendo que hormônio de crescimento (43%, esteroides sexuais (30%, tiroxina (11,9% e oxandrolona (9,5% foram os mais utilizados. A comparação da idade da gestação no momento em que ocorreu o parto com dificuldade no aprendizado mostrou razão de prevalência de 1,71 (p>0,05. CONCLUSÃO: a implantação baixa dos cabelos é o estigma mais prevalente nas pacientes com cariótipo 45,X e as alterações clínicas mais comuns são as cardiovasculares, otites, hipertensão arterial e disfunções tireoidianas, porém não apresentam correlação com o cariótipo.PURPOSE: to examine the association between cytogenetic characteristics and clinical and epidemiological changes in patients with Turner syndrome (TS. METHODS: Forty-two patients were included. Data were collected using a standardized

SISTEMA CONFEA/CREA APROVA O CURSO SUPERIOR DE TECNOLOGIA EM GESTÃO AMBIENTAL DA UNISULVIRTUAL E CRIA O TÍTULO DE TECNÓLOGO EM GESTÃO AMBIENTAL NA LISTA DE TÍTULOS DO SISTEMA.

OpenAIRE

Henkes, Jairo Afonso

2015-01-01

O Confea (Conselho Federal de Engenharia e Agronomia) aprovou em Reunião Plenária de 25 e 26 de junho de 2015, a Criação e  Inclusão do Título de Tecnólogo em Gestão Ambiental na lista de Títulos Profissionais do Sistema Confea/Crea, e na mesma Sessão Plenária também aprovou o Registro do Curso Superior de Tecnologia em Gestão Ambiental da Unisul Virtual, no Sistema, sendo o primeiro curso EaD do Brasil a receber este registro, uma luta da Coordenação de Curso e da UnisulVirtual desde sua con...

Identification of 4,5-didemethyl-4-O-alpha-D-glucopyranosylsimmondsin and pinitol alpha-D-galactosides in jojoba seed meal (Simmondsia chinensis).

Science.gov (United States)

Van Boven, M; Leyssen, T; Busson, R; Holser, R; Cokelaere, M; Flo, G; Decuypere, E

2001-09-01

The isolation and identification of two pinitol alpha-D-galactosides from jojoba meal are described. The products were isolated by a combination of preparative HPLC on silica gel and TLC on amino silica gel and were identified by MS, NMR spectroscopy, and chemical derivatization as 5-O-(alpha-D-galactopyranosyl)-3-O-methyl-D-chiro-inositol or 5-alpha-D-galactopyranosyl-D-pinitol and 2-O-(alpha-D-galactopyranosyl)-3-O-methyl-D-chiro-inositol or 2-alpha-D-galactopyranosyl-D-pinitol. The same preparative HPLC method on silica gel allowed a new simmondsin derivative to be isolated and identified as 4,5-didemethyl-4-O-alpha-D-glucopyranosylsimmondsin mainly by NMR spectroscopy and high-resolution mass spectrometry.

Quantum Cascade Laser Measurements of Line Intensities, N2-, O2- and Ar- Collisional Broadening Coefficients of N2O in the  3 Band Near 4.5  m

KAUST Repository

Es-sebbar, Et-touhami; Deli, Meriem; Farooq, Aamir

2016-01-01

This study deals with precise measurements of absolute line intensities, N2-, O2- and Ar- collisional broadening coefficients of N2O in the P-branch of the ν3 vibrational band near 4.5 μm. Collisional broadening coefficients of N2O-air are derived from the N2- and O2- broadening contributions by considering an ideal atmospheric composition. Studies are performed at room temperature for 10 rotational transitions over 2190-2202 cm-1 spectral range using a distributed-feedback quantum cascade laser. To retrieve spectroscopic parameters for each individual transition, measured absorption line shape is simulated within Voigt and Galatry profiles. The obtained results compare well with previous experimental data available in the literature: the discrepancies being less than 4% for most of the probed transitions. The spectroscopic data reported here are very useful for the design of sensors used to monitor the abundance of N2O in earth's atmosphere. © The Author(s) 2016.

Quantum Cascade Laser Measurements of Line Intensities, N2-, O2- and Ar- Collisional Broadening Coefficients of N2O in the  3 Band Near 4.5  m

KAUST Repository

Es-sebbar, Et-touhami

2016-04-19

This study deals with precise measurements of absolute line intensities, N2-, O2- and Ar- collisional broadening coefficients of N2O in the P-branch of the ν3 vibrational band near 4.5 μm. Collisional broadening coefficients of N2O-air are derived from the N2- and O2- broadening contributions by considering an ideal atmospheric composition. Studies are performed at room temperature for 10 rotational transitions over 2190-2202 cm-1 spectral range using a distributed-feedback quantum cascade laser. To retrieve spectroscopic parameters for each individual transition, measured absorption line shape is simulated within Voigt and Galatry profiles. The obtained results compare well with previous experimental data available in the literature: the discrepancies being less than 4% for most of the probed transitions. The spectroscopic data reported here are very useful for the design of sensors used to monitor the abundance of N2O in earth\\'s atmosphere. © The Author(s) 2016.

Epidemiological importance of humans and domestic animals as reservoirs of verocytotoxin-producing Escherichia coli

Directory of Open Access Journals (Sweden)

Lazić Srđan

2006-01-01

Full Text Available Background/Aim. A "new" pathogenic agent, verocytotoxin - producing Escherichia coli (VTEC emerged in the last 20 years, causing an increased number of sporadic cases, as well as of outbreaks of diarrhoeal diseases. Humans and animals can be infected with VTEC, but their epidemiological importance as a reservoir of this agent is not quite clear, especially in the Balkan region. Therefore, the aim of this study was to investigate the frequency of isolation of VTEC from the intestinal tract of humans and animals and to determine the serogroups of the isolated strains. Methods. A total of, 3 401 stool samples from humans and 2 660 samples from five different species of domestic animals were tested for the presence of this pathogen. Results. VTEC was isolated from 20 (0.6% humans stools and from 431 (16.2% animal fecal samples (p < 0.001. Only 15 (3.3% VTEC strains belonged to human infection-associated serogroups (O26, O55, O111, O128 and O 157, designated as enterohaemorrhagic E. coli (EHEC. The most known serogroup- O157 was identified in 6 (1.3% of the isolated VTEC strains; of them, 1 (5% was of human origin and 5 (1.2% were animal strains. Conclusion. This study revealed that domestic animals were a more important reservoir of VTEC than humans, and that the isolated VTEC strains rarely belonged to O157, as well as to other EHEC serogroups that might explain rare sporadic cases and the absence of epidemic occurrence of diarrhoeal diseases caused by VTEC in this geographic region.

Produção de leite de vacas alimentadas com alta proporção de forragem em dietas

Directory of Open Access Journals (Sweden)

Moreira V.R.

2003-01-01

Full Text Available Vinte e duas vacas primíparas e 26 multíparas da raça Holandesa foram distribuídas em três tratamentos em um delineamento inteiramente ao acaso. As dietas testadas consistiram de duas proporções forragem:concentrado, 55:45 (RCS e 75:25 (RCSH, para silagem de milho comum, e 75:25 (BMR para outra dieta baseada no híbrido bm3. Não houve interação entre tratamentos e ordem de lactação. A proporção silagem de alfafa:silagem de milho na porção forrageira da dieta foi de 47,7:53,3. A ingestão (kg/dia de matéria seca e de proteína bruta foi superior para BMR e RCS (19,5 e 19,5; 3,41 e 3,42, respectivamente em relação à ingestão para RCSH (17,6 e 3,14, enquanto que a de fibra em detergente neutro foi maior para BMR (6,61 e menor para RCSH e RCS (6,08 e 5,40, respectivamente. O consumo de fibra em detergente ácido (kg/dia foi maior para BMR e RCSH (4,88 e 4,73 e menor para RCS (4,02. A produção de leite foi superior para o tratamento com maior proporção de concentrados (35,7kg/vaca/dia, seguida pelo tratamento BMR (34,1kg/vaca/dia e finalmente por RCSH, com a menor produção (32,1kg/vaca/dia. O teor de gordura no leite foi superior nos tratamentos com alto conteúdo de forragem na dieta, enquanto que a porcentagem de proteína seguiu padrão oposto. O híbrido bm3, em dieta contendo alta proporção de forragem, foi eficiente em manter o nível de desempenho de vacas de alta produção em comparação à dietas com relações forragem:concentrado normal ou alta e baseadas em híbridos contendo diferente genética de milho.

Masculinização de larvas de tilápia do Nilo (Oreochromis niloticus por banhos de imersão e o andrógeno dissolvido em solução de dimetilsulfóxido (DMSO - DOI: 10.4025/actascianimsci.v26i2.1867 Masculinizaton of Nile tilápia fries (Oreochromis niloticus through bath immersion and androgen dissolved in dimethilsulfoxide (DMSO solution - DOI: 10.4025/actascianimsci.v26i2.1867

Directory of Open Access Journals (Sweden)

Fábio Meurer

2004-04-01

Full Text Available O experimento constituiu-se de dois ensaios. O primeiro objetivou determinar a concentração de dimetilsulfóxido não-prejudicial às larvas de tilápia do Nilo (Oreochromis niloticus L. para utilização em banhos de imersão, e o segundo avaliou a masculinização e o desempenho zootécnico das larvas submetidas à imersão em diferentes concentrações hormonais. Os animais foram imersos por 36 horas em recipientes de 0,5L, com 50 larvas. No primeiro ensaio, utilizaram-se as concentrações de 0,04%; 0,1%; 0,3%; 0,7% e 1,0% de dimetilsulfóxido, não apresentando diferença significativa (p>0,05 sobre resultados de sobrevivência entre os tratamentos. No segundo ensaio, utilizaram-se os tratamentos de 0,5; 1,5; 2,5; 3,5; 4,5 e 5,5mg de 17alpha-metiltestosterona.L-1, em solução de 0,04% de dimetilsulfóxido, verificando-se melhor resultado (p-1.This experiment was composed of two essays. The first one studied the deletrious effect of dimethylsulfoxide concentration on Nile tilapia (Oreochromis niloticus L. fries through bath immersion. The second assessed the sex reversal of male and the performance of Nile tilapia fries submitted to immersion in different hormone concentrations. The animals were immersed over 36 hours in recipients of 0.5L, with fifty fries. In the first essay dimethylsulfoxide concentrations of 0.04, 0.1, 0.3, 0.7 and 1.0% were utilized and there was no significant difference (p>0.05 in survival results between the treatments. In the second essay 0.5, 1.5, 2.5, 3.5, 4.5 and 5.5mg of 17alpha-metyltestosterone.L-1 were utilized in 0.04% dimethylsulfoxide solution, in which the best result (P-1

Magnetic and transport properties of Zn0.4Fe2.6O4 thin films with highly preferential orientation

International Nuclear Information System (INIS)

Lu, Z.L.; Zou, W.Q.; Liu, X.C.; Lin, Y.B.; Lu, Z.H.; Wang, J.F.; Xu, J.P.; Lv, L.Y.; Zhang, F.M.; Du, Y.W.

2007-01-01

Highly preferentially oriented Zn 0.4 Fe 2.6 O 4 thin films have been fabricated on Si, SrTiO 3 and ZrO 2 substrates, respectively, using RF magnetron sputtering. All the films show a large saturation magnetization of about 4.2μ B and low coercive field at 300 K and a spin (cluster) glass transition at about 60 K due to the non-magnetic Zn 2+ ions substitution. Moreover, the fairly high spin polarization of the carrier at 300 K has been confirmed by both the giant magnetoresistance and anomalous Hall coefficient measurements

CHARACTERIZATION OF COMMERCIALLY AVAILABLE ALKALI RESISTANT GLASS FIBER FOR CONCRETE REINFORCEMENT AND CHEMICAL DURABILITY COMPARISON WITH SrO-Mn2O3-Fe2O3-MgO-ZrO2-SiO2 (SMFMZS SYSTEM GLASSES

Directory of Open Access Journals (Sweden)

Göktuğ GÜNKAYA

2012-12-01

Full Text Available According to the relevant literature, the utilization of different kind of glass fibers in concrete introduces positive effect on the mechanical behavior, especially toughness. There are many glassfibers available to reinforce concretes. Glass fiber composition is so important because it may change the properties such as strength, elastic modulus and alkali resistance. Its most important property to be used in concrete is the alkali resistance. Some glasses of SrO–MgO–ZrO2–SiO2 (SMZS quaternary system, such as 26SrO, 20MgO, 14ZrO2, 40SiO2 (Zrn glass, have been found to be highly alkali resistant thanks to their high ZrO2 and MgO contents. Previous researches on these glasses with MnO and/or Fe2O3 partially replacing SrO have been made with the aim of improving the chemical resistance and decreasing the production cost.The main target of the present study, first of all, was to characterize commercially available alkali resistant glass fiber for concrete reinforcement and then to compare its alkali durability with those of the SrO-Mn2O3-Fe2O3-MgO-ZrO2-SiO2 (SMFMZS system glasses. For such purposes, XRF, Tg-DTA, alkali resistance tests and SEM analysis conducted with EDX were employed. According tothe alkali endurance test results it was revealed that some of the SMFMZS system glass powders are 10 times resistant to alkali environments than the commercial glass fibers used in this study.Therefore, they can be considered as alternative filling materials on the evolution of chemically resistant concrete structures.

Effect of MnO2 doping and temperature treatment on optical energy band gap properties in Zn-Bi-Ti-O varistor ceramics

International Nuclear Information System (INIS)

Ghazali, M. S. M.; Abdullah, W. R. W.; Zakaria, A.; Kamari, H. M.; Rizwan, Z.

2016-01-01

In this study, the optical band-gap energy ( Eg ) was investigated with respect to MnO 2 and sintering temperatures on ZnO based varistor ceramics. Eg of the ceramic (99-x) mol% ZnO + 0.5 mol% Bi 2 O 3 + 0.5 mol% TiO 2 + × MnO 2 where × = 0, 0.2, 0.4, 0.6 and 0.8 mol%, were determined using UV-Vis spectrophotometer. The samples was prepared through solid-state route and sintered at the sintering temperature from 1110, 1140 and 1170 °C for 45 and 90 min in open air. At no doping of MnO 2 , the values of Eg are 2.991 ± 0.001, 2.989 ± 0.001 eV for 45 and 90 min sintering time; respectively. Eg was decreased to 2.192 ± 0.001 eV at 1140 °C at 45 min sintering time. Similar result of Eg was observed at longer heat treatment. Further addition of dopant causing the Eg decreases rapidly to 2.099 and 2.106 ± 0.001 eV at 45 and 90 min sintering time; respectively. XRD analysis indicates that there is hexagonal ZnO and secondary phases, Zn 2 MnO 4 , Bi 4 Ti 3 O 12 and Zn 2 Ti 3 O 8 . The relative density of the sintered ceramics decreased or remain constant with the increase of MnO 2 concentration for 45 min sintering time, however, further prolong sintering time; the relative density decreases form 90.25 to 88.35%. This indicates the pores are increasing with the increase of heat treatment. The variation of sintering temperatures to the optical band gap energy of based ZnO varistor doped with MnO 2 due to the formation of interface states. (paper)

Diagnóstico da geração e da composição dos RCD de Fortaleza/CE Generation and composition diagnosis of C&D waste of Fortaleza/CE

Directory of Open Access Journals (Sweden)

Maria Elane Dias de Oliveira

2011-09-01

Full Text Available Este trabalho objetivou apresentar os resultados de um diagnóstico anual da geração e da composição dos resíduos de construção e demolição de Fortaleza. Durante 12 meses, foram colhidas informações das empresas coletoras e da prefeitura, tais como a quantidade e o preço do metro cúbico do entulho coletado e o destino dado a ele. Foram ainda coletadas amostras de resíduos de construção e demolição nos locais de descarte licenciados pela prefeitura. Os resultados apontam que os locais licenciados recebem em média 702 toneladas.dia-1, sendo que a composição desses resíduos de construção e demolição é, em média, de 65% de restos de argamassa, concreto e material cerâmico. Constatou-se também que grande parte desse tipo de resíduo é descartado ilegalmente, sendo que os dois principais aterros irregulares somam uma área aproximada de 26 hectares. Estima-se que esse sistema movimenta R$ 4,5 milhões anualmente e que quase metade desse montante é aportado somente pela Prefeitura Municipal.This article aimed to present the results of an annual diagnosis of generation and composition of Fortaleza’s construction and demolition waste. During 12 months CDW collection companies’ and the city hall’ information were researched, such as the amount and the price of the cubic meter of the collected wastes and the destiny given to them. Samples of construction and demolition waste were either collected at the licensed disposal places. The results point that licensed places receive 702 tons.day-1, on average, and the composition of this construction and demolition waste is made, on average, by 65% of mortar, concrete and ceramic material. It was also verified that great part of this waste is discarded illegally, and the two main irregular disposal sites have an approximate area of 26 hectares. It is estimated that system costs approximately R$4.5 million annually and almost half of this amount is paid only by

Anisotropy of the irreversibility field for Zr-doped $(Y,Gd)Ba_2Cu_3O_{7-x}$ thin films up to 45T

OpenAIRE

Tarantini, C.; Jaroszynski, J.; Kametani, F.; Zuev, Y. L.; Gurevich, A.; Chen, Y.; Selvamanickam, V.; Larbalestier, D. C.; Christen, D. K.

2012-01-01

The anisotropic irreversibility field B$_{Irr}$ of two $YBa_2Cu_3O_{7-x}$ thin films doped with additional rare earth (RE)=(Gd,Y) and Zr and containing strong correlated pins (splayed BaZrO$_{3}$ nanorods, and $RE_2O_3$ nanoprecipitates), has been measured over a very broad range up to 45T at temperatures 56 K

Shiga toxin-producing Escherichia coli isolated from chicken meat in Iran: serogroups, virulence factors, and antimicrobial resistance properties.

Science.gov (United States)

Momtaz, Hassan; Jamshidi, Alireza

2013-05-01

The aim of the current study was to determine the virulence factors, serogroups, and antibiotic resistance properties of Shiga toxin-producing Escherichia coli isolated from chicken meat samples. A total of 422 chicken meat samples were collected from 5 townships of Iran. Specimens were immediately transferred to the laboratory in a cooler with an ice pack. Samples were cultured, and the positive culture samples were analyzed by PCR assays. Finally, the antimicrobial susceptibility test was performed using the disk diffusion method in Mueller-Hinton agar. According to the results, out of 422 samples, 146 (34.59%) were confirmed to be E. coli positive and among E. coli-positive samples, 51 (34.93%) and 31 (21.23%) were from attaching and effacing E. coli (AEEC) and enterohemorrhagic E. coli (EHEC) subgroups, respectively. All of the EHEC-positive samples had all stx1, eaeA, and ehly virulence genes, whereas only 5 (9.80%) of AEEC subgroup had all stx1, stx2, and eaeA genes. As the data revealed, O157 was the most prevalent and O111 was the least prevalent strains in the Shiga toxin-producing E. coli (STEC) population. Among STEC strains, sulI and blaSHV had the highest and lowest incidence rate, respectively. There was a high resistance to tetracycline (76.82%), followed by chloramphenicol (73.17%) and nitrofurantoin (63.41%), but there was low resistance to cephalotine (7.31%) antibiotics in isolated strains. Results shows that the PCR technique has a high performance for detection of serogroups, virulence genes, and antibiotic resistance genes in STEC strains. This study is the first prevalence report of detection of virulence genes, serogroups, and antibiotic resistance properties of STEC strains isolated from chicken meat samples in Iran. Based on the results, chicken meat is one of the main sources of STEC strains and its virulence factors in Iran, so an accurate meat inspection would reduce disease outbreaks.

Assessment for the role of rare earth oxide in the R2O3 - RuO2 - Pt composite electrode

International Nuclear Information System (INIS)

Do Ngoc Lien; Nguyen Van Sinh

2004-01-01

Our work has showed several results related to assessment for the role of rare earth oxide in the R 2 O 3 - RuO 2 - Pt composite electrode. The precursor method was used for preparing composite electrode in the following forms: a- RuO 2 - Pt electrode b- La 2 O 3 (55%) - RuO 2 (45%) - Pt electrode c- CeO 2 (60%) - RuO 2 (40%) - Pt electrode By measurements of anodic polarization and cyclic potential for the types of a, b, c electrodes we can see that the La 2 O 3 (55%) - 45% RuO 2 - Pt electrode will be the best anodic electrode. It means that the partial replacement of ruthenium oxide by lanthanum oxide in composite oxide electrode will be an effective one. (author)

Uropathogenic Escherichia coli pathogenicity islands and other ExPEC virulence genes may contribute to the genome variability of enteroinvasive E. coli.

Science.gov (United States)

da Silva, Laís Cristina; de Mello Santos, Ana Carolina; Silva, Rosa Maria

2017-03-16

Enteroinvasive Escherichia coli (EIEC) may be the causative agent of part of those million cases of diarrhea illness reported worldwide every year and attributable to Shigella. That is because both enteropathogens have many common characteristics that difficult their identification either by traditional microbiological methods or by molecular tools used in the clinical laboratory settings. While Shigella has been extensively studied, EIEC remains barely characterized at the molecular level. Recent EIEC important outbreaks, apparently generating more life-threatening cases, have prompted us to screen EIEC for virulence traits usually related to extraintestinal pathogenic E. coli (ExPEC). That could explain the appearance of EIEC strains presenting higher virulence potential. EIEC strains were distributed mainly in three phylogroups in a serogroup-dependent manner. Serogroups O124, O136, O144, and O152 were exclusively classified in phylogroup A; O143 in group E; and O28ac and O29 in group B1. Only two serogroups showed diverse phylogenetic origin as follows: O164 was assigned to groups A, B1, C, and B2 (one strain each), and O167 in groups E (five strains), and A (one strain) (Table 1). Eleven of 20 virulence genes (VGs) searched were detected, and the majority of the 19 different VGs combinations found were serogroup-specific. Uropathogenic E. coli (UPEC) PAI genetic markers were detected in all EIEC strains. PAIs I J96 and II CFT073 were the most frequent (92.1 and 80.4%, respectively). PAI IV 536 was restricted to some serogroups from phylogroups A, B1 and E. PAI I CFT073 was uniquely detected in phylogroups B2 and E. A total of 45 (88%) strains presented multiple PAI markers (two to four). PAIs I J96 and II CFT073 were found together in 80% of strains. EIEC is a DEC pathovar that presents VGs and pathogenicity island genetic markers typically associated with ExPEC, especially UPEC. These features are distributed in a phylogenetic and serogroup-dependent manner

Relação entre a infusão contínua de dexmedetomidina e a fração expirada de sevoflurano monitorizada pelo índice bispectral

Directory of Open Access Journals (Sweden)

Magalhães Edno

2004-01-01

Full Text Available JUSTIFICATIVA E OBJETIVOS: A associação de agentes venosos à anestesia geral inalatória proporciona melhor qualidade de analgesia e hipnose. A dexmedetomidina é um agonista específico alfa2-adrenérgico com estas características e reconhecida por proporcionar estabilidade hemodinâmica. O objetivo do estudo foi avaliar o efeito da infusão contínua de dexmedetomidina na fração expirada de sevoflurano (FEsevo em anestesia geral, monitorizada pela análise bispectral do EEG (BIS. MÉTODO: Estudou-se, de modo prospectivo, 24 pacientes adultos, de ambos os sexos, estado físico ASA I e II, submetidos à cirurgias eletivas sob anestesia geral, monitorizados com ECG, PANI, SpO2, P ET CO2, FEsevo e BIS. Procedeu-se a indução venosa com alfentanil (30 µg.kg-1, tiopental (5 mg.kg-1 e vecurônio (0,1 mg.kg-1 e intubação traqueal. Iniciaram-se a administração de sevoflurano (manutenção do BIS entre 40 e 60, a ventilação controlada com volume corrente de 10 ml.kg-1 e a manutenção da P ET CO2 entre 35 e 45 mmHg. Na fase de manutenção da anestesia, após 60 minutos da indução anestésica (M60, foi iniciada a infusão contínua de dexmedetomidina em 2 fases: infusão inicial (1 µg.kg-1 em 20 minutos; e, infusão de manutenção (0,5 µg.kg-1.h-1. A concentração de sevoflurano foi ajustada para manter o valor de BIS entre 40 e 60. As variáveis de PA, FC, FEsevo, SpO2, P ET CO2 e BIS foram avaliadas nos momentos pré-indução (M-15, M15, M45, M75, M105 e M120. RESULTADOS: A associação da dexmedetomidina à anestesia geral com sevoflurano proporcionou redução significativa (p < 0,05 da FEsevo de M45 (1,604 ± 0,485 a M105 (1,073 ± 0,457 e de M45 (1,604 ± 0,485 a M120 (1,159 ± 0,475. As variáveis hemodinâmicas analisadas apresentaram diferenças estatisticamente significantes com p < 0,05, mas sem repercussões clínicas. CONCLUSÕES: A associação de dexmedetomidina em infusão contínua (0,5 µg.kg-1.h-1 à anestesia

Photoelectrochemical oxidation of ibuprofen via Cu_2O-doped TiO_2 nanotube arrays

International Nuclear Information System (INIS)

Sun, Qiannan; Peng, Yen-Ping; Chen, Hanlin; Chang, Ken-Lin; Qiu, Yang-Neng; Lai, Shiau-Wu

2016-01-01

Highlights: • A p–n junction material was synthesized to enhance photocatalytic ability. • Cu_2O-doped TiO_2 nanotube arrays works as a photoanode in a PEC system. • Recombination of photo-generated holes and electrons were greatly reduced. • Synergetic effect was quantified in PEC degradation. • Recombination of photogenerated holes and electrons was greatly enhanced. - Abstract: A p–n junction based Cu_2O-doped TiO_2 nanotube arrays (Cu_2O-TNAs) were synthesized and used as a working anode in a photoelectrochemical (PEC) system. The results revealed that the Cu_2O-TNAs were dominated by the anatase phase and responded significantly to visible light. XPS analyses indicated that with an amount of 24.79% Cu doping into the structure, the band gap of Cu_2O-TNAs was greatly reduced. SEM images revealed that the supported TiO_2 nanotubes had diameters of approximately 80 nm and lengths of about 2.63 μm. Upon doping with Cu_2O, the TiO_2 nanotubes maintained their structural integrity, exhibiting no significant morphological change, favoring PEC applications. Under illumination, the photocurrent from Cu_2O/TNAs was 2.4 times larger than that from TNAs, implying that doping with Cu_2O significantly improved electron mobility by reducing the rate of recombination of electron-hole pairs. The EIS and Bode plot revealed that the estimated electron lifetimes, τ_e_l, of TNAs and Cu_2O/TNAs were 6.91 and 26.26 ms, respectively. The efficiencies of degradation of Ibuprofen by photoelectrochemical, photocatalytic (PC), electrochemical (EC) and photolytic (P) methods were measured.

Microscopic fungi in the Atlantic Rainforest in Cubatão, São Paulo, Brazil Fungos microscópicos de Mata Atlântica em Cubatão, São Paulo, Brasil

Directory of Open Access Journals (Sweden)

Iracema Helena Schoenlein-Crusius

2006-09-01

Full Text Available This article presents a survey of fungi obtained from soil, water and mixed leaf litter samples taken from the Atlantic Rainforest in the municipality of Cubatão, in the State of São Paulo, during the years of 1993 to 1995. Using different techniques for the isolation of microscopic fungi, a total of 280 taxa was obtained (66 zoosporic fungi, 40 Mucorales, 45 Glomales, 125 anamorphs, three Ascomycota and one Basidiomycota, with 23 species being reported for the first time in Brazil.Este artigo apresenta o levantamento dos fungos obtidos de amostras de solo, água e folhedo mixto coletados da Mata Atlântica no município de Cubatão, estado de São Paulo, durante os anos de 1993 a 1995. Utilizando diferentes técnicas para isolamento de fungos microscópicos, um total de 280 táxons foram obtidos (66 fungos zoospóricos, 40 representantes de Mucorales, 45 de Glomales, 125 fungos anamorfos, três de Ascomycota e um representante de Basidiomycota, sendo 23 espécies reportadas pela primeira vez para o Brasil.

Efeito da idade e da suplementação oral com o acetato de DL-alfa-tocoferol sobre os níveis séricos de vitamina E e sobre o metabolismo oxidativo dos neutrófilos em bezerros

Directory of Open Access Journals (Sweden)

J. N. Costa

2006-03-01

Full Text Available Este trabalho teve por objetivo avaliar o efeito da idade e da suplementação oral com acetato de DL-alfa-tocoferol sobre os níveis séricos de vitamina E e sobre o metabolismo oxidativo dos neutrófilos de bezerros da raça Jersey do nascimento aos 45 dias de idade. Foram utilizados 14 bezerros divididos em dois grupos de sete animais cada. O grupo tratamento recebeu 2g de acetato de DL-alfa-tocoferol, por via oral, diariamente, até os 45 dias de vida e o controle não recebeu nenhum tipo de suplementação para esta vitamina. Foram colhidas amostras de soro sanguíneo ao nascimento, aos cinco, 10, 20, 30 e 45 dias de idade, para: determinação sérica da vitamina E e para a avaliação da função dos neutrófilos. Concluímos que a suplementação oral com acetato de DL-alfa-tocoferol, na dose de 2 g/dia/VO, do nascimento aos 45 dias de idade aumentou significativamente (p≤ 0,05 os níveis séricos de vitamina E em bezerros. Os menores percentuais de neutrófilos redutores foram detectados logo após o nascimento e os maiores aos 45 dias de idade. A suplementação com vitamina E não alterou significativamente a função oxidativa dos neutrófilos, mas os valores superiores obtidos para os animais do grupo tratado, para o teste estimulado, sugerem uma ação neutrófilica mais potente diante uma infecção natural nesses animais.

15,17-Di-2-propenylcalix[4]arene-25,26,27,28-tetrol: self-complexation and C-H...O interactions

NARCIS (Netherlands)

Harkema, Sybolt; van Loon, Jan-Dirk; van Loon, Jan-Dirk; Verboom, Willem; Reinhoudt, David

1998-01-01

The crystal structure of 15,17-di-2-propenylcalix[4]arene-25,26,27,28-tetrol [systematic name: 15,17-di-2-propenylpentacyclo[19.3.1.13,7.19,13.115,19]octacosa-1(25), 3,5,7(26),9,11,13 (27),15,17,19 (28),21,23-dodecaene-25,26,27,28-tetrol], C34H32O4, was determined. The calix[4]arene molecule shows a

Prevalência da exposição ao fumo ambiental do tabaco em casa e do tabagismo na população Portuguesa – o estudo INAsma

Directory of Open Access Journals (Sweden)

A.M. Pereira

2013-05-01

Full Text Available Resumo: Introdução: Neste estudo, pretendemos: 1 estimar a prevalência, na população portuguesa, da exposição ao fumo ambiental do tabaco em casa; 2 estimar a prevalência de tabagismo em Portugal; 3 identificar as características sociais e pessoais associadas ao tabagismo ou à exposição ao fumo ambiental do tabaco. Métodos: Estudo transversal consistindo na aplicação, à população, a nível nacional, de questionário telefónico. Completaram a entrevista 6 003 indivíduos. A exposição ao fumo ambiental do tabaco em casa foi definida como a exposição, em casa, ao fumo do tabaco de, pelo menos, um fumador atual. Por fumador entendeu-se um indivíduo com ≥ 15 anos que fumou, pelo menos, um cigarro por dia durante um período de um ano; um fumador atual fumou no último mês. Resultados: Referiram exposição ao fumo ambiental do tabaco em casa 26,6% dos participantes (IC 95%: 25,5-27,7. Viver num agregado familiar constituído por ≥ 4 pessoas (OR = 2,31; IC 95%: [1,81-2,96], ser fumador atual (OR = 7,29; IC 95%: [5,74-9,26] ou ter asma atual (OR = 2,06; IC 95%: [1,45-2,94] associaram-se positivamente à exposição ao fumo ambiental do tabaco. Na análise estratificada por sexo, o efeito da asma atual manteve-se apenas nas mulheres.Atualmente, 19,0% (IC 95%: 18,0-20,0 da população portuguesa é fumadora e 17,2% (IC 95%: 16,2-18,2 são ex-fumadores. A prevalência de fumadores atuais é mais elevada nos homens do que nas mulheres (26,5 versus 12,2%, p < 0,001. A probabilidade de ser um fumador atual foi maior nos homens, nas pessoas mais instruídas e nos indivíduos expostos, em casa, ao fumo ambiental do tabaco. Na análise estratificada por sexo, o efeito da escolaridade manteve-se apenas nas mulheres. Conclusão: A exposição ao fumo ambiental do tabaco em casa foi mais elevada do

In-vitro study of copper doped SiO{sub 2}-CaO-P{sub 2}O{sub 5} system for bioactivity and antimicrobial properties

Energy Technology Data Exchange (ETDEWEB)

Kaur, Kulwinder; Singh, K. J., E-mail: kanwarjitsingh@yahoo.com; Anand, Vikas [Department of Physics, Guru Nanak Dev University, Amritsar-143005 (India); Kaur, Harpreet; Arora, Daljit Singh [Department of Microbiology, Guru Nanak Dev University, Amritsar-143005 (India)

2015-08-28

Samples of the xCuO-(45-x)CaO-10P{sub 2}O{sub 5}-45SiO{sub 2} system (x varies from 0 to 4 mole%) have been synthesized for application as biomaterials to slow or inhibit the growth of living organisms (fungi and other pathogenic microorganisms) by the combination of sol-gel and co-precipitation processes. Prepared samples have been characterized by X-Ray Diffraction, Fourier Transform Infra-Red and Field Emission Scanning Electron Microscopy techniques before and after immersion in simulated body fluid. Antimicrobial activity of samples has been investigated against Staphylococcus aureus. Releasing of Cu{sup 2+}and other ions in the simulated body fluid has been determined by Atomic Absorption Spectroscopy to ensure the use of prepared material as biomaterial with good antibacterial properties.

Evaluation of mericon E. coli O157 Screen Plus and mericon E. coli STEC O-Type Pathogen Detection Assays in Select Foods: Collaborative Study, First Action 2017.05.

Science.gov (United States)

Bird, Patrick; Benzinger, M Joseph; Bastin, Benjamin; Crowley, Erin; Agin, James; Goins, David; Armstrong, Marcia

2018-05-01

QIAGEN mericon Escherichia coli O157 Screen Plus and mericon E. coli Shiga toxin-producing E. coli (STEC) O-Type Pathogen Detection Assays use Real-Time PCR technology for the rapid, accurate detection of E. coli O157 and the "big six" (O26, O45, O103, O111, O121, O145) (non-O157 STEC) in select food types. Using a paired study design, the assays were compared with the U.S. Department of Agriculture, Food Safety Inspection Service Microbiology Laboratory Guidebook Chapter 5.09 reference method for the detection of E. coli O157:H7 in raw ground beef. Both mericon assays were evaluated using the manual and an automated DNA extraction method. Thirteen technicians from five laboratories located within the continental United States participated in the collaborative study. Three levels of contamination were evaluated. Statistical analysis was conducted according to the probability of detection (POD) statistical model. Results obtained for the low-inoculum level test portions produced a difference between laboratories POD (dLPOD) value with a 95% confidence interval of 0.00 (-0.12, 0.12) for the mericon E. coli O157 Screen Plus with manual and automated extraction and mericon E. coli STEC O-Type with manual extraction and -0.01 (-0.13, 0.10) for the mericon E. coli STEC O-Type with automated extraction. The dLPOD results indicate equivalence between the candidate methods and the reference method.

Comparison of the fate of the top six non-O157 shiga-toxin producing Escherichia coli (STEC) and E. coli O157:H7 during the manufacture of dry fermented sausages.

Science.gov (United States)

Balamurugan, S; Ahmed, Rafath; Gao, Anli; Strange, Phil

2017-10-16

The study examined the relative fate of the top six non-O157 shiga-toxin producing Escherichia coli (STEC) and E. coli O157:H7 during the manufacture of dry fermented sausages (DFS). Three separate batches of sausages containing a five-strain cocktail for each serogroup and uninoculated control were manufactured and subjected to identical fermentation, maturation and dry curing conditions. Changes in physicochemical properties and inoculated STEC numbers were enumerated during the DFS production stages and log reduction and log reduction rates were calculated. Inoculation of very high concentrations (8logCFUg -1 ) of STEC in the sausage batter did not significantly (P>0.05) affect the changes in the pH, a w , moisture, protein, fat content compared to the uninoculated DFS. There was a significant (P0.05) from each other. These results indicate that the lethality of DFS production processes observed against E. coli O157:H7 would result in a similar inactivation of the top six non-O157 STEC. Crown Copyright © 2017. Published by Elsevier B.V. All rights reserved.

Effect of various SnO2 pH on ZnO/SnO2-composite film via immersion technique

Science.gov (United States)

Malek, M. F.; Mohamed, R.; Mamat, M. H.; Ismail, A. S.; Yusoff, M. M.; Rusop, M.

2018-05-01

ZnO/SnO2-composite film has been synthesized via immersion technique with various pH of SnO2. The pH of SnO2 were varied between 4.5 and 6.5. The optical measurements of the samples were carried out using Varian Cary 5000 UV-Vis spectrophotometer within the range from 350 nm to 800 nm at room temperature in air with a data interval of 1 nm. On the other hand, the optical photoluminescence properties were measured by a photoluminescence spectrometer (PL, model: Horiba Jobin Yvon - 79 DU420A-OE-325) using a He-Cd laser as the excitation source at 325 nm. These highly oriented ZnO/SnO2-composite film are potential for the creation of functional materials, such as the sensors, solar cells and etc.

Distribution of Leptospira serogroups in cattle herds and dogs in France.

Science.gov (United States)

Ayral, Florence C; Bicout, Dominique J; Pereira, Helena; Artois, Marc; Kodjo, Angeli

2014-10-01

A retrospective study was conducted to identify and describe the distribution pattern of Leptospira serogroups in domestic animals in France. The population consisted of cattle herds and dogs with clinically suspected leptospirosis that were tested at the "Laboratoire des Leptospires" between 2008 and 2011. The laboratory database was queried for records of cattle and dogs in which seroreactivity in Leptospira microagglutination tests was consistent with a recent or current infection, excluding vaccine serogroups in dogs. A total of 394 cattle herds and 232 dogs were diagnosed with clinical leptospirosis, and the results suggested infection by the Leptospira serogroup Australis in 43% and 63%, respectively; by the Leptospira serogroup Grippotyphosa in 17% and 9%, respectively; and by the Leptospira serogroup Sejroe in 33% and 6%, respectively. This inventory of infecting Leptospira serogroups revealed that current vaccines in France are not fully capable of preventing the clinical form of the disease. © The American Society of Tropical Medicine and Hygiene.

Travel-associated infections caused by unusual serogroups of Legionella pneumophila identified using Legionella BIOCHIP slides in Turkey and Iraq.

Science.gov (United States)

Kocazeybek, Bekir S; Yuksel, Pelin; Keskin, Dilek; Sheikh, Suhail; Habip, Zafer; Yavuzer, Serap Sahin; Caliskan, Reyhan; Altun, Yagız Meric; Kuskucu, Mert; Cengiz, Mahir; Dinc, Harika Oyku; Karakullukcu, Asiye; Ergin, Sevgi; Saribas, Suat; Yilmaz, Nail; Tokman, Hrisi Bahar

2016-01-01

Although Legionella pneumophila serogroup 1 is the common disease causing serogroup, rare serogroups can also may cause legionellosis. A 54-year-old male patient (index case) reported that he had been on a religious trip (for visiting, tomb of Ali, which is important for Shias) to Iraq with a large group (50 shia pilgrims from Kars city of Turkey) two weeks prior to admission. Due to civil war, the hotel where the patient stayed in Iraq lacked proper hygiene. A large number of people in the travel group were experiencing the same symptoms. Other five cases were 2 males (ages; 50, 45) and 3 females including the wife of the index case (ages; 50, 28, 27). The detection of L. pneumophila IgG and IgM was performed by anti-L. pneumophila Indirect Immunofluorescent IgM, IgG kit. Legionella 1 biochip/verification BIOCHIP slides were used for serogrouping in Euroimmun AG, Leubeck, Germany. In index case, L. pneumophila IgM was positive with a titer of 1/32 titer. IgG was negative with a 1/100 titer. Another case (28 year old female), had clinical symptoms identical to the index case. L. pneumophila IgM and IgG were positive with titers of 1/64 and 1/100, respectively. These two cases were diagnosed with Legionnaires' disease caused by L. pneumophila serogroup 12 (index case) and female (28-year-old) by serogroup 11. The other 4 cases were diagnosed with possible Pontiac fever caused by L. pneumophila serogroups 14 (wife of the index case), 4, and 6 whereas the serogroup of L. pneumophila detected in 27 years old female case could not be identified. A major limitation of this work is the absence of genotyping and the serogroup difference between index case and his wife who shared the same hotel. We suggest that this serogroup difference may be caused by (for men and women) sitting separately in Islamic rules. On the other hand, the movement of people in the context of mutual visits between countries or neighboring countries for tourism-related (i.e., for religious events

Design of flexible PANI-coated CuO-TiO2-SiO2 heterostructure nanofibers with high ammonia sensing response values

Science.gov (United States)

Pang, Zengyuan; Nie, Qingxin; Lv, Pengfei; Yu, Jian; Huang, Fenglin; Wei, Qufu

2017-06-01

We report a room-temperature ammonia sensor with extra high response values and ideal flexibility, including polyaniline (PANI)-coated titanium dioxide-silicon dioxide (TiO2-SiO2) or copper oxide-titanium dioxide-silicon dioxide (CuO-TiO2-SiO2) composite nanofibers. Such flexible inorganic TiO2-SiO2 and CuO-TiO2-SiO2 composite nanofibers were prepared by electrospinning, followed by calcination. Then, in situ polymerization of aniline monomers was carried out with inorganic TiO2-SiO2 and CuO-TiO2-SiO2 composite nanofibers as templates. Gas sensing tests at room temperature indicated that the obtained CuO-TiO2-SiO2/PANI composite nanofibers had much higher response values to ammonia gas (ca. 45.67-100 ppm) than most of those reported before as well as the prepared TiO2-SiO2/PANI composite nanofibers here. These excellent sensing properties may be due to the P-N, P-P heterojunctions and a structure similar to field-effect transistors formed on the interfaces between PANI, TiO2, and CuO, which is p-type, n-type, and p-type semiconductor, respectively. In addition, the prepared free-standing CuO-TiO2-SiO2/PANI composite nanofiber membrane was easy to handle and possessed ideal flexibility, which is promising for potential applications in wearable sensors in the future.

[Zn(phen)(O,N,O)(H2O)] and [Zn(phen)(O,N)(H2O)] with O,N,O is 2,6-dipicolinate and N,O is L-threoninate: synthesis, characterization, and biomedical properties.

Science.gov (United States)

Chin, Lee-Fang; Kong, Siew-Ming; Seng, Hoi-Ling; Tiong, Yee-Lian; Neo, Kian-Eang; Maah, Mohd Jamil; Khoo, Alan Soo-Beng; Ahmad, Munirah; Hor, Tzi-Sum Andy; Lee, Hong-Boon; San, Swee-Lan; Chye, Soi-Moi; Ng, Chew-Hee

2012-10-01

Two ternary Zn(II) complexes, with 1,10-phenanthroline (phen) as the main ligand and a carboxylate-containing ligand [dipicolinate (dipico) or L-threoninate (L-Thr)] as the subsidiary ligand, were prepared and characterized by elemental analysis, Fourier transform IR, UV, and fluorescence spectroscopy, X-ray diffraction, molar conductivity, and electrospray ionization mass spectrometry. X-ray structure analysis shows that both [Zn(phen)(dipico)(H(2)O)]·H(2)O (1) and [Zn(phen)(L-Thr)(H(2)O)Cl]·2H(2)O (2) have octahedral geometry about the Zn(II) atom. Both complexes can inhibit topoisomerase I, and have better anticancer activity than cisplatin against nasopharyngeal cancer cell lines, HK1 and HONE-1, with concentrations causing 50 % inhibition of cell proliferation (IC(50)) in the low micromolar range. Complex 2 has the highest therapeutic index for HK1. Both Zn(II) complexes can induce cell death by apoptosis. Changing the subsidiary ligand in the Zn(II) complexes affects the UV-fluorescence spectral properties of the coordinated phen ligand, the binding affinity for some DNA sequences, nucleobase sequence-selective binding, the phase at which cell cycle progression was arrested for treated cancer cells, and their therapeutic index.

Genetic Diversity of the fliC Genes Encoding the Flagellar Antigen H19 of Escherichia coli and Application to the Specific Identification of Enterohemorrhagic E. coli O121:H19

Science.gov (United States)

Beutin, Lothar; Delannoy, Sabine

2015-01-01

Enterohemorrhagic Escherichia coli (EHEC) O121:H19 belong to a specific clonal type distinct from other classical EHEC and major enteropathogenic E. coli groups and is regarded as one of the major EHEC serogroups involved in severe infections in humans. Sequencing of the fliC genes associated with the flagellar antigen H19 (fliCH19) revealed the genetic diversity of the fliCH19 gene sequences in E. coli. A cluster analysis of 12 fliCH19 sequences, 4 from O121 and 8 from non-O121 E. coli strains, revealed five different genotypes. All O121:H19 strains fell into one cluster, whereas a second cluster was formed by five non-O121:H19 strains. Cluster 1 and cluster 2 strains differ by 27 single nucleotide exchanges in their fliCH19 genes (98.5% homology). Based on allele discrimination of the fliCH19 genes, a real-time PCR test was designed for specific identification of EHEC O121:H19. The O121 fliCH19 PCR tested negative in 73 E. coli H19 strains that belonged to serogroups other than O121, including 28 different O groups, O-nontypeable H19, and O-rough:H19 strains. The O121 fliCH19 PCR reacted with all 16 tested O121:H19 strains and 1 O-rough:H19 strain which was positive for the O121 wzx gene. A cross-reaction was observed only with E. coli H32 strains which share sequence similarities in the target region of the O121 fliCH19 PCR. The combined use of O-antigen genotyping (O121 wzx) and the detection of O121 fliCH19 allele type contributes to improving the identification and molecular serotyping of EHEC O121:H19 motile and nonmotile strains and variants of these strains lacking stx genes. PMID:25862232

Temperature-programmed reaction of CO2 reduction in the presence of hydrogen over Fe/Al2O3, Re/Al2O3 and Cr-Mn-O/Al2O3 catalysts

International Nuclear Information System (INIS)

Mirzabekova, S.R.; Mamedov, A.B.; Krylov, O.V.

1996-01-01

Regularities in CO 2 reduction have been studied using the systems Fe/Al 2 O 3 , Re/Al 2 O 3 and Cr-Mn-O/Al 2 O 3 under conditions of thermally programmed reaction by way of example. A sharp increase in the reduction rate in the course of CO 2 interaction with reduced Fe/Al 2 O 3 and Re/Al 2 O 3 , as well as with carbon fragments with addition in CO 2 flow of 1-2%H 2 , has been revealed. The assumption is made on intermediate formation of a formate in the process and on initiating effect of hydrogen on CO 2 reduction by the catalyst. Refs. 26, figs. 10

Análise de tratamento anti-rábico humano pós-exposição em região da Grande São Paulo, Brasil

Directory of Open Access Journals (Sweden)

Rita C. M. Garcia

1999-06-01

Full Text Available INTRODUÇÃO: O tratamento de seres humanos expostos ao risco de infecção pelo vírus rábico ainda pode incluir a ocorrência de reações pós-vacinais indesejáveis, tanto de ordem local como geral. A análise sistemática dos informes epidemiológicos de pacientes submetidos a este tipo de tratamento poderá oferecer subsídios para a modificação desta situação. Foram analisados os registros de tratamento dessa zoonose visando à melhoria do seu controle. MÉTODOS: Foram analisadas através do programa Epi Info as fichas de investigação epidemiológica da raiva de 8.758 habitantes do Município de Osasco, SP (Brasil, atendidos no período de 1984 a 1994. A vacina utilizada foi do tipo Fuenzalida & Palacios. RESULTADOS: Constatou-se a existência de maior risco de exposição para os indivíduos do sexo masculino, com cinco a nove anos de idade. As agressões ocorreram com maior freqüência no domicílio da vítima e os cães foram os principais responsáveis. Dos cães e gatos envolvidos, respectivamente 51,0% e 73,2% não haviam sido imunizados contra a raiva. Nos pacientes com até nove anos de idade as localizações de lesão mais freqüentes foram: cabeça (36,6% e membros superiores (35,1%; quando a faixa etária ultrapassava os nove anos as áreas mais acometidas foram membros superiores (45,8% e membros inferiores (43,7%. Dos pacientes analisados, 26,5% já haviam recebido vacinação anti-rábica anterior e 90, 7% procurou a orientação médica em até cinco dias da agressão. Para 41,9% foi prescrita unicamente a vacinação e para 0,05 ‰ a soro-vacinação. CONCLUSÕES: Houve 11,7% de abandonos a tratamentos e 51,3% foram dispensados do mesmo em função da observação animal. Dos pacientes tratados com vacina ou soro-vacinação houve 0,25% de acidentes pós-vacinais, dos quais 0,3‰ do tipo neurológico. Os meses de março, julho, agosto e setembro foram os de maior procura.

Análise de tratamento anti-rábico humano pós-exposição em região da Grande São Paulo, Brasil

Directory of Open Access Journals (Sweden)

Garcia Rita C. M.

1999-01-01

Full Text Available INTRODUÇÃO: O tratamento de seres humanos expostos ao risco de infecção pelo vírus rábico ainda pode incluir a ocorrência de reações pós-vacinais indesejáveis, tanto de ordem local como geral. A análise sistemática dos informes epidemiológicos de pacientes submetidos a este tipo de tratamento poderá oferecer subsídios para a modificação desta situação. Foram analisados os registros de tratamento dessa zoonose visando à melhoria do seu controle. MÉTODOS: Foram analisadas através do programa Epi Info as fichas de investigação epidemiológica da raiva de 8.758 habitantes do Município de Osasco, SP (Brasil, atendidos no período de 1984 a 1994. A vacina utilizada foi do tipo Fuenzalida & Palacios. RESULTADOS: Constatou-se a existência de maior risco de exposição para os indivíduos do sexo masculino, com cinco a nove anos de idade. As agressões ocorreram com maior freqüência no domicílio da vítima e os cães foram os principais responsáveis. Dos cães e gatos envolvidos, respectivamente 51,0% e 73,2% não haviam sido imunizados contra a raiva. Nos pacientes com até nove anos de idade as localizações de lesão mais freqüentes foram: cabeça (36,6% e membros superiores (35,1%; quando a faixa etária ultrapassava os nove anos as áreas mais acometidas foram membros superiores (45,8% e membros inferiores (43,7%. Dos pacientes analisados, 26,5% já haviam recebido vacinação anti-rábica anterior e 90, 7% procurou a orientação médica em até cinco dias da agressão. Para 41,9% foi prescrita unicamente a vacinação e para 0,05 ‰ a soro-vacinação. CONCLUSÕES: Houve 11,7% de abandonos a tratamentos e 51,3% foram dispensados do mesmo em função da observação animal. Dos pacientes tratados com vacina ou soro-vacinação houve 0,25% de acidentes pós-vacinais, dos quais 0,3‰ do tipo neurológico. Os meses de março, julho, agosto e setembro foram os de maior procura.

Temperature-controlled formation of Anderson-type compounds and their conversion to [γ-Mo{sub 8}O{sub 26}]{sup 4-}-based variants using pendent ligands

Energy Technology Data Exchange (ETDEWEB)

Tian, Ai-xiang; Li, Ting-ting; Tian, Yan; Ni, Huai-ping; Ji, Xue-bin; Liu, Jia-ni; Liu, Guo-cheng; Ying, Jun [Bohai Univ., Jinzhou (China). Dept. of Chemistry

2017-10-01

By tuning the reaction temperature, two Anderson- and two [γ-Mo{sub 8}O{sub 26}]{sup 4-}-based compounds decorated by pendent organic ligands, [Cu{sup II}{sub 9}(bpz){sub 2}(pz){sub 2}(H{sub 2}O){sub 24}]-[H{sub 2}(Cr(OH){sub 5}Mo{sub 6}O{sub 19}){sub 4}].11H{sub 2}O (1), [Cu{sup II}(bpz){sub 2}(H{sub 2}O){sub 2}(γ-H{sub 4}-Mo{sub 8}O{sub 26})].2H{sub 2}O (2), [Cu{sup II}{sub 2}(tea){sub 2}(H{sub 2}O){sub 6}(HCr(OH){sub 6}Mo{sub 6}O{sub 18}){sub 2}]. 6H{sub 2}O (3) and [Ag{sup I}(bpz)(H{sub 2}O)(γ-H{sub 4}Mo{sub 8}O{sub 26}){sub 0.5}] (4) (bpz=4-butyl-1H-pyrazole, pz=1H-pyrazole, tea=2-[1,2,4]triazol-4-yl-ethylamine), have been hydrothermally synthesized and characterized by single-crystal X-ray diffraction analysis, IR spectra and elemental analyses. In compound 1, there are two kinds of tri-nuclear Cu{sup II} clusters induced by bpz and pz ligands, respectively. Four Anderson-type anions are linked by these tri-nuclear clusters to form a ''W''-type subunit. In compound 2, the [Cu(bpz){sub 2}(H{sub 2}O){sub 2}]{sup 2+} subunits connect the γ-Mo{sub 8} anions to construct a chain. The remaining two non-coordinated N donors in [Cu(bpz){sub 2}(H{sub 2}O){sub 2}]{sup 2+} further link two adjacent γ-Mo{sub 8} anions through Mo-N bonds. In compound 3, there exists a bi-nuclear Cu{sup II} cluster [Cu{sub 2}(tea){sub 2}(H{sub 2}O){sub 6}]{sup 4+}. The discrete bi-nuclear Cu{sup II} clusters and the CrMo{sub 6} anions link each other through abundant hydrogen bonding interactions. In compound 4, the [Ag(bpz)(H{sub 2}O)]{sup +} subunits connect γ-Mo{sub 8} anions to build a zigzag chain. The chains are further fused by other [Ag(bpz)(H{sub 2}O)]{sup +} cations to form a grid-like layer. There still exist Mo-N bonds in 4. We also have investigated the electrochemical and photocatalytic properties of 1-4.

Mobilização corporal para prevenção de úlceras por pressão: custo direto com pessoal

Directory of Open Access Journals (Sweden)

Antônio Fernandes Costa Lima

2015-10-01

Full Text Available RESUMOObjetivo:calcular o custo total médio (CTM relativo à mão de obra direta (MOD de profissionais de enfermagem para a mobilização corporal de pacientes visando à prevenção de úlceras por pressão.Método:estudo de caso quantitativo, exploratório-descritivo. Observou-se a realização de 656 mobilizações preventivas e calculou-se o custo multiplicando-se o tempo despendido pelos profissionais pelo custo unitário da MOD.Resultados:o CTM com MOD por Unidade correspondeu a: Clínica Médica R$ 5,38 por mudança de decúbito, R$ 5,26 por posicionamento em poltrona, R$ 5,55 por auxílio deambulação; Clínica Cirúrgica R$ 2,42 por mudança de decúbito, R$ 2,30 por posicionamento em poltrona, R$ 2,96 por auxílio deambulação e Unidade de Terapia Intensiva R$ 8,15 por mudança de decúbito, R$ 7,57 por posicionamentos em poltrona, R$ 15,32 por auxílio deambulação.Conclusão:o conhecimento gerado poderá subsidiar o gerenciamento de custos relacionados aos recursos humanos necessários ao cuidado de enfermagem eficiente e eficaz.

Aplicação da radiação gama na desinfestação de rações a base de grãos para alimentação de animais domésticos

OpenAIRE

Amanda Cristina Ramos Koike

2009-01-01

O presente trabalho teve como objetivos realizar um levantamento para identificar a entomofauna associada a rações na cidade São Paulo/SP bem como avaliar o efeito da radiação gama em rações para alimentação de animais domésticos infestadas por pragas. Amostras de 20 lojas Pet Shop de diferentes regiões da cidade São Paulo/SP foram submetidas a triagens de 1 e 45 dias para coleta de insetos com auxilio de aspirador entomológico e peneiras de diferentes granulometrias. As espécies Sitophilus z...

Resolution of 1-n-butyl-3-methyl-3-phospholene 1-oxide with TADDOL derivatives and calcium salts of O,O'-Dibenzoyl-(2R,3R)- or O,O'-di-p-toluoyl-(2R,3R)-tartaric acid.

Science.gov (United States)

Bagi, Péter; Fekete, András; Kállay, Mihály; Hessz, Dóra; Kubinyi, Miklós; Holczbauer, Tamás; Czugler, Mátyás; Fogassy, Elemér; Keglevich, György

2014-03-01

The resolution methods applying (-)-(4R,5R)-4,5-bis(diphenylhydroxymethyl)-2,2-dimethyldioxolane ("TADDOL"), (-)-(2R,3R)-α,α,α',α'-tetraphenyl-1,4-dioxaspiro[4.5]decan-2,3-dimethanol ("spiro-TADDOL"), as well as the acidic and neutral Ca(2+) salts of (-)-O,O'-dibenzoyl- and (-)-O,O'-di-p-toluoyl-(2R,3R)-tartaric acid were extended for the preparation of 1-n-butyl-3-methyl-3-phospholene 1-oxide in optically active form. In one case, the intermediate diastereomeric complex could be identified by single-crystal X-ray analysis. The absolute P-configuration of the enantiomers of the phospholene oxide was also determined by comparing the experimentally obtained and calculated CD spectra. © 2014 Wiley Periodicals, Inc.

Facile synthesis of core-shell Cu2O@ ZnO structure with enhanced photocatalytic H2 production

Science.gov (United States)

Zhang, Yong-Hui; Jiu, Bei-Bei; Gong, Fei-Long; Lu, Kuan; Jiang, Nan; Zhang, Hao-Li; Chen, Jun-Li

2018-05-01

Core-shell Cu2O@ZnO composites were synthesized successfully based on a one-pot hydrothermal method in the presence of dioctyl sulfosuccinate sodium salt (AOT) surfactant. The Cu2O can be converted to rough core-shell Cu2O@ZnO structure by adjusting the amount of zinc powder added. The as-synthesized Cu2O@ZnO composites exhibited excellent photocatalytic activity and the amount of H2 generated using these composites was 4.5-fold more than that produced with Cu2O cubes. A possible photocatalytic mechanism for the Cu2O@ZnO composites with enhanced photocatalytic activity could be the separation by ZnO of the effective charge carriers.

Dependence of Photocatalytic Activity of TiO2-SiO2 Nanopowders

Directory of Open Access Journals (Sweden)

M. Riazian

2014-10-01

Full Text Available Structural properties and chemical composition change the photocatalytic activity in TiO2-SiO2 nanopowder composite. The SiO2-TiO2 nanostructure is synthesized based on sol–gel method. The nanoparticles are characterized by x-ray fluorescents (XRF, x- ray diffraction (XRD, tunneling electron microscopy (TEM, field emission scanning electron microscopy (FE-SEM, UV-vis. Spectrophotometer and furrier transmission create infrared absorption (FTIR techniques. The rate constant k for the degradation of methylen blue in its aqueous solution under UV irradiation is determined as a measure of photocatalytic activity. Dependence between photocatalytic activity and SiO2 content in the composite is determined. Rate constant k is found dependent on the content of SiO2 in the composite that calcined at 900 oC. The addition of low composition SiO2 to the TiO2 matrix (lower than 45% enhances the photocatalytic activity due to thermal stability and increasing in the surface area. The effects of chemical compositions on the surface topography and the crystallization of phases are studied.

Analgesia preemptiva com dipirona versus ibuprofeno na fotocoagulação a laser da retina

Directory of Open Access Journals (Sweden)

Artur Macaferri Del Santo

2016-02-01

Full Text Available RESUMO Objetivo: Comparar a efetividade da dipirona versus o ibuprofeno para a redução da dor na fotocoagulação da retina (FR. Método: Foi realizado um estudo controlado, duplo cego e randomizado englobando trinta e quatro pacientes com retinopatia diabética separados em dois grupos. Grupo A recebeu 1000mg de dipirona e o grupo B recebeu 600mg de ibuprofeno. A dor foi avaliada pela escala visual analógica. Resultado: A média do escore da dor nos grupos A e B foi de 5,2 ± 2.6 e 4,5 ± 1,4, respectivamente. Não houve diferença estatística entre os grupos (p=0,34. O mesmo foi observado quanto à analise entre dor leve, moderada e grave (p=0,09. Os grupos foram semelhantes quanto à idade e sexo. Conclusão: Os medicamentos foram equivalentes ou equipotentes em reduzir a dor ocular durante a fotocoagulação a laser da retina.

Genetic characterization of antibiotic resistance in enteropathogenic Escherichia coli carrying extended-spectrum beta-lactamases recovered from diarrhoeic rabbits.

Science.gov (United States)

Poeta, P; Radhouani, H; Gonçalves, A; Figueiredo, N; Carvalho, C; Rodrigues, J; Igrejas, G

2010-05-01

A total of 52 Escherichia coli strains isolated from diarrhoeic rabbits were investigated for their enteropathogenic E. coli (EPEC) pathotype by PCR amplification of eae and bfp virulence genes. A total of 22 EPEC isolates were identified, serotyped and studied for antibiotic resistance and screened for the detection of extended-spectrum beta-lactamases (ESBLs). The EPEC isolates belonged to three serogroups (O26, O92 and O103). The most common serogroup (O103:K-:H2) was observed among 17 EPEC strains, the O92:K-serogroup in three isolates (the antibiotic sensitive ones) and the remaining O26:K-serogroup in two isolates (the ESBLs isolates). Resistances to ampicillin and tetracycline were the most frequent and detected followed by resistance to nalidixic acid, streptomycin, trimethoprim-sulphamethoxazole, cefoxitin, gentamicin and ciprofloxacin. All the isolates were sensitive for amikacin, ceftazidime, aztreonam, imipenem, chloramphenicol, tobramycin and amoxicillin + clavulanic acid. Two isolates recovered from two adult animals showed an intermediate susceptibility to cefotaxime, and a positive screening test for ESBL was demonstrated in both. The bla(TEM) gene was demonstrated in the majority of ampicillin-resistant isolates. The aac(3)-II or aac(3)-IV genes were detected in the four gentamicin-resistant isolates. In addition, the aadA gene was detected in 60% of streptomycin-resistant isolates. The tet(A) or tet(B) genes were identified in all tetracycline-resistant isolates. A total of nine EPEC isolates showed the phenotype SXT-resistant, and the sul1 and/or sul2 and/or sul3 genes were detected in all of them. Our findings showed that the molecular detection by the eae and bfp genes by PCR followed by serotyping is useful for monitoring trends in EPEC infections of rabbits allowing the identification of their possible reservoirs. The detection of genes involved in the resistance to antibiotics of different families in a relatively high proportion of faecal E

Crescimento inicial e absorção de zinco pelo milho em função do modo de aplicação e fontes do nutriente

Directory of Open Access Journals (Sweden)

C. A. Rosolem

1998-03-01

Full Text Available Freqüentemente, têm-se obtido aumentos na produção de grãos de milho em resposta ao Zn, em solos brasileiros. O objetivo do presente trabalho foi comparar fontes e modos de aplicação de Zn à cultura do milho. O experimento foi desenvolvido em casa de vegetação, em vasos de polietileno com capacidade de 10 litros, utilizando-se amostras de um Latossolo Vermelho-Escuro de textura média. Aplicou-se calcário para se atingir 70% de saturação do solo por bases. Os tratamentos consistiram da aplicação de zinco como óxido, sulfato, EDTA e lignossulfonado, na semente (90 g ha-1, no sulco de semeadura (5,3 kg ha-1 e incorporado (5,3 kg ha-1. As plantas foram colhidas 45 dias após a emergência. A aplicação de zinco via semente é eficiente no fornecimento de Zn para o crescimento das plantas até os 45 dias. A incorporação, independentemente da fonte, e o zinco aplicado como EDTA e lignossulfonado proporcionam maior disponibilidade do nutriente ao milho. O crescimento do sistema radicular é prejudicado quando há muito Zn disponível na zona de crescimento. A dose de 5,3 kg ha-1 de zinco como EDTA ou lignossulfonado, quando aplicada no sulco de semeadura, é fitotóxica ao milho.

Cool-cultivated red leaf lettuce accumulates cyanidin-3-O-(6″-O-malonyl)-glucoside and caffeoylmalic acid.

Science.gov (United States)

Becker, Christine; Klaering, Hans-Peter; Kroh, Lothar W; Krumbein, Angelika

2014-03-01

Cultivating lettuce in greenhouses at low temperatures improves its CO2-balance and may increase its content of flavonoid glycosides and phenolic acids. We cultivated 5weeks old red leaf lettuce seedlings at 20/15°C (day/night) or 12/7°C until plants reached comparable growth stages: small heads were harvested after 13 (warm) and 26 (cool)days, while mature heads were harvested after 26 (warm) or 52 (cool)days. Additionally, some plants were cultivated first cool then warm and vice versa (39days). Cool-cultivated small heads had higher concentrations of cyanidin-3-O-(6″-O-malonyl)-glucoside and caffeoylmalic acid than warm-cultivated ones but we detected no differences concerning quercetin and luteolin glycosides or di-O-caffeoyltartaric and 5-O-caffeoylquinic acid. Regarding mature heads, there were only differences concerning cyanidin-3-O-(6″-O-malonyl)-glucoside. We therefore suggest that only cyanidin-3-O-(6″-O-malonyl)-glucoside was truly responsive to temperatures alone. Previously reported contrasting effects may rather be due to comparison of different growth stages or interactive effects with radiation. Copyright © 2013 The Authors. Published by Elsevier Ltd.. All rights reserved.

O-serotyping of Escherichia coli Strains isolated from Patients With Urinary Tract Infection in Southeast of Iran

Directory of Open Access Journals (Sweden)

Ahmad Rashki

2014-11-01

Full Text Available Background: Uropathogenic Escherichia coli (UPEC O-serogroups with their phylogenetic background are the most prevalent causes of urinary tract infections (UTIs. Objectives: The association of O types with phylogenetic background was assessed among E. coli isolates collected from patients with UTI. Patients and Methods: In this study, 186 patients with UTI, referred to two hospitals affiliated to Zabol University of Medical Sciences in southeast of Iran, were enrolled during January to July 2013. Phylogenetic groups and serotyping were performed using multiplex-PCR method. Results: A total of 100 E. coli strains were isolated from the urine samples. The most common types of O antigens were O2 (16.43%, O6 (16.43% and O18 (13.69%. The phylogenetic analysis showed that 63 O-antigen-positive isolates were mainly segregated from the phylogenetic group B2 (56% and the substantial prevalence (30% belonged to the phylogenetic group D. Conclusions: This was the first report of E. coli serotyping in patient with UTI from southeast of Iran as well as investigation of their relation with phylogenetic pattern by multiplex-PCR. Further studies from other parts of Iran and on other serotypes are recommended.

Recuperação da desnutrição em ratos mediante rações adicionadas ou não de suplemento alimentar e de vitaminas e minerais durante o período de crescimento

Directory of Open Access Journals (Sweden)

Guzmán-Silva Maria Angélica

2004-01-01

Full Text Available OBJETIVOS: O propósito deste estudo foi analisar experimentalmente a eficiência da suplementação alimentar da dieta do Município de Quissamã, RJ, em recuperar a desnutrição durante o período de crescimento. MÉTODOS: Foram utilizados 42 Rattus norvegicus, desmamados ao 26º dia e induzidos à desnutrição por 21 dias (ração hipoprotéica 2%, ad libitum. Os animais foram distribuídos em sete grupos, alimentados com suas respectivas dietas, todas isoprotéicas (10% e isoenergéticas (350Kcal/100g. Foi registrado o peso dos animais e, após o sacrifício, foram coletados e pesados fígado, rins, baço e intestino. RESULTADOS: O ganho ponderal médio no 28º dia do controle suplemento alimentar (75,33g foi significativamente inferior aos demais grupos, e o do controle suplemento alimentar e vitaminas+minerais foi o mais elevado (213,17. Quase todos os grupos diferenciaram-se estatisticamente no peso médio de fígado, rins e baço; os órgãos do controle suplemento alimentar apresentaram o menor peso (respectivamente 3,34g; 0,97g; 0,24g sendo significativamente inferior aos demais grupos. O controle suplemento alimentar e vitaminas+minerais apresentou o maior peso médio do fígado (13,85g. O Controle vitaminas+minerais apresentou o maior peso médio dos rins (1,88g e do baço (0,87g. O número de placas de Peyer/animal variou de nove a treze, sem diferenças entre os grupos; o tamanho das placas de Peyer do controle suplemento alimentar (2,6mm foi significativamente inferior aos demais grupos. O Quissamã suplemento alimentar apresentou as maiores placas de Peyer (4,4mm. O controle suplemento alimentar apresentou sempre valores inferiores para todos os parâmetros estudados comparativamente, já que não foram adicionados à sua ração vitaminas e minerais. CONCLUSÃO: A dieta de Quissamã, RJ experimentalmente alcança as necessidades mínimas para promover a recuperação da desnutrição, sendo desnecessária a adição de

Padrão de distribuição da calda produzida pela ponta de pulverização do tipo jato plano (8002 em função do espaçamento entre bicos Spray distribution pattern of flat fan tip (8002 at different spacing between nozzles

Directory of Open Access Journals (Sweden)

A.L.S. Lacerda

2001-12-01

Full Text Available O presente trabalho foi desenvolvido no laboratório de Engenharia do Departamento de Engenharia Rural da Escola Superior de Agricultura "Luiz de Queiroz" (USP/ESALQ, Piracicaba-SP, com o objetivo de estudar o padrão de distribuição da ponta de pulverização do tipo jato plano (leque 8002, em função das distâncias entre os bicos. Para isso, foram colocados 10 bicos sobre uma mesa de prova, um de cada vez, a uma altura fixa de 45 cm, nos espaçamentos de 10 a 80 cm. Os volumes coletados em cada uma das canaletas serviram para calcular o coeficiente de variação e iniciar o estudo do espaçamento adequado. Após os testes realizados, pôde-se concluir que o bico tipo jato plano 8002 mostrou melhor distribuição em espaçamento de 50 cm, com menor coeficiente de variação (6,88% a uma altura fixa de 45 cm e pressão regulada a 279,3 Kpa, registrada no manômetro.This work was carried out at the Department of Agricultural Engineering, Escola Superior de Agricultura "Luiz de Queiroz" (USP/ESALQ, Piracicaba-SP, Brazil. The objective of this research was to study the spray pattern of the flat fan tip (8002 at different spacings between nozzles. Thus, ten nozzles were placed on a tilted foil, one at a time, at a fixed height of 45 cm, with spacings varying from 10 to 80 cm. Nozzle output was collected separately from each nozzle and used to calculate the variation coefficient and determine the more appropriate spacing between nozzles. It was concluded that, 50 cm-spaced flat fan tips (8002 showed the best spray pattern with a smaller variation coefficient at a fixed height of 45 cm and pressure regulated at 279.3 kPa registered in the manometer.

Synthesis of 5-O-α- and -β-D-glucopyranosyl-D-glucofuranose and 5-O-α-D-glucopyranosyl-D-fructopyranose (leucrose)

NARCIS (Netherlands)

Vliegenthart, J.F.G.; Heeswijk, W.A.R. van; Wassenburg, F.R.

1978-01-01

Reaction of 1,2-O-cyclopentylidene-α-D-glucofuranurono-6,3-lactone (2) with 2,3,4,6-tetra-O-acetyl-α-D-glucopyranosyl bromide (1) gave 1,2-O-cyclopentylidene- 5-O-(2,3,4,6-tetra-O-acetyl-α-D-glucopyranosyl)-α-D-glucofuranurono-6,3-lactone (3, 45%) and

Hydrothermal synthesis and structural analysis of new mixed oxyanion borates: Ba11B26O44(PO4)2(OH)6, Li9BaB15O27(CO3) and Ba3Si2B6O16

Science.gov (United States)

Heyward, Carla; McMillen, Colin D.; Kolis, Joseph

2013-07-01

Several new borate compounds, Ba11B26O44(PO4)2(OH)6 (1), Li9BaB15O27(CO3) (2), and Ba3Si2B6O16 (3) were synthesized containing other hetero-oxyanion building blocks in addition to the borate frameworks. They were all prepared under hydrothermal conditions and characterized by single crystal and powder X-ray diffraction, and IR spectroscopy. Crystal data: For 1; space group P21/c, a=6.8909 (14) Å, b=13.629 (3) Å, c=25.851 (5) Å, β=90.04 (3)°; For 2; space group P-31c, a=8.8599 (13) Å, c=15.148 (3) Å; For 3; space group P-1, a=5.0414 (10) Å, b=7.5602 (15) Å, c=8.5374 (17) Å, α=77.15 (3)°, β=77.84 (3)°, γ=87.41 (3)° for 3. Compounds 1 and 2 contain isolated oxyanions [PO4]3- and [CO3]2- respectively, sitting in channels created by the borate framework, while structure 3 has the [SiO4]4- groups directly bonded to the borate groups creating a B-O-Si framework.

Syntheses, structures, and properties of Ag4(Mo2O5)(SeO4)2(SeO3) and Ag2(MoO3)3SeO3

International Nuclear Information System (INIS)

Ling Jie; Albrecht-Schmitt, Thomas E.

2007-01-01

Ag 4 (Mo 2 O 5 )(SeO 4 ) 2 (SeO 3 ) has been synthesized by reacting AgNO 3 , MoO 3 , and selenic acid under mild hydrothermal conditions. The structure of this compound consists of cis-MoO 2 2+ molybdenyl units that are bridged to neighboring molybdenyl moieties by selenate anions and by a bridging oxo anion. These dimeric units are joined by selenite anions to yield zigzag one-dimensional chains that extended down the c-axis. Individual chains are polar with the C 2 distortion of the Mo(VI) octahedra aligning on one side of each chain. However, the overall structure is centrosymmetric because neighboring chains have opposite alignment of the C 2 distortion. Upon heating Ag 4 (Mo 2 O 5 )(SeO 4 ) 2 (SeO 3 ) looses SeO 2 in two distinct steps to yield Ag 2 MoO 4 . Crystallographic data: (193 K; MoKα, λ=0.71073 A): orthorhombic, space group Pbcm, a=5.6557(3), b=15.8904(7), c=15.7938(7) A, V=1419.41(12), Z=4, R(F)=2.72% for 121 parameters with 1829 reflections with I>2σ(I). Ag 2 (MoO 3 ) 3 SeO 3 was synthesized by reacting AgNO 3 with MoO 3 , SeO 2 , and HF under hydrothermal conditions. The structure of Ag 2 (MoO 3 ) 3 SeO 3 consists of three crystallographically unique Mo(VI) centers that are in 2+2+2 coordination environments with two long, two intermediate, and two short bonds. These MoO 6 units are connected to form a molybdenyl ribbon that extends along the c-axis. These ribbons are further connected together through tridentate selenite anions to form two-dimensional layers in the [bc] plane. Crystallographic data: (193 K; MoKα, λ=0.71073 A): monoclinic, space group P2 1 /n, a=7.7034(5), b=11.1485(8), c=12.7500(9) A, β=105.018(1) V=1002.7(2), Z=4, R(F)=3.45% for 164 parameters with 2454 reflections with I>2σ(I). Ag 2 (MoO 3 ) 3 SeO 3 decomposes to Ag 2 Mo 3 O 10 on heating above 550 deg. C. - Graphical abstract: A view of the one-dimensional [(Mo 2 O 5 )(SeO 4 ) 2 (SeO 3 )] 4- chains that extend down the c-axis in the structure of Ag 4 (Mo 2 O 5 )(SeO 4

A multiple antibiotic and serum resistant oligotrophic strain, Klebsiella pneumoniae MB45 having novel dfrA30, is sensitive to ZnO QDs

Directory of Open Access Journals (Sweden)

Chakrabarti Pinak

2011-05-01

Full Text Available Abstract Background The aim of this study was to describe a novel trimethoprim resistance gene cassette, designated dfrA30, within a class 1 integron in a facultatively oligotrophic, multiple antibiotic and human serum resistant test strain, MB45, in a population of oligotrophic bacteria isolated from the river Mahananda; and to test the efficiency of surface bound acetate on zinc oxide quantum dots (ZnO QDs as bactericidal agent on MB45. Methods Diluted Luria broth/Agar (10-3 media was used to cultivate the oligotrophic bacteria from water sample. Multiple antibiotic resistant bacteria were selected by employing replica plate method. A rapid assay was performed to determine the sensitivity/resistance of the test strain to human serum. Variable region of class 1 integron was cloned, sequenced and the expression of gene coding for antibiotic resistance was done in Escherichia coli JM 109. Identity of culture was determined by biochemical phenotyping and 16S rRNA gene sequence analyses. A phylogenetic tree was constructed based on representative trimethoprim resistance-mediating DfrA proteins retrieved from GenBank. Growth kinetic studies for the strain MB45 were performed in presence of varied concentrations of ZnO QDs. Results and conclusions The facultatively oligotrophic strain, MB45, resistant to human serum and ten antibiotics trimethoprim, cotrimoxazole, ampicillin, gentamycin, netilmicin, tobramycin, chloramphenicol, cefotaxime, kanamycin and streptomycin, has been identified as a new strain of Klebsiella pneumoniae. A novel dfr gene, designated as dfrA30, found integrated in class 1 integron was responsible for resistance to trimethoprim in Klebsiella pneumoniae strain MB45. The growth of wild strain MB45 was 100% arrested at 500 mg/L concentration of ZnO QDs. To our knowledge this is the first report on application of ZnO quantum dots to kill multiple antibiotics and serum resistant K. pneumoniae strain.

Gestão Ambiental Urbana: o caso da Prefeitura Municipal de Ipiranga-PR

Directory of Open Access Journals (Sweden)

Arquimedes Szezerbicki

2005-06-01

Full Text Available O presente estudo, dentro da área da Gestão Ambiental da  Engenharia de Produção, está situado na subárea Políticas Ambientais. O objetivo é discutir como o poder público da cidade de Ipiranga–PR vem executando um projeto de Aterro Controlado e como as condições de vida da população urbanas do município são alteradas. Para a consecução do objetivo proposto, além de pesquisa bibliográfica e  documental, foram realizadas observações sistemáticas para a composição do corpus documental da pesquisa. O método empregado foi o indutivo. Verificou-se que o município de Ipiranga adotou um modelo de aterro controlado, reduzindo seu custo operacional. As pesquisas realizadas indicaram que a vida útil projetada dos aterros é de 26,5 anos. Houve uma economia  considerável na construção do sistema, dispensando a construção de sistemas mais complexos. Conclui-se que a idealização do projeto de construção dos aterros controlados do tipo trincheiras de pequenas dimensões, beneficia tanto a população quanto o meio ambiente, visando à economia do município.

Avaliação de diferentes programas de restrição alimentar para matrizes pesadas

OpenAIRE

Carneiro, Paulo Roberto de Oliveira [UNESP

2013-01-01

Em matrizeiros é comum a utilização de regimes de restrição alimentar para o controle de peso das aves. Objetivou-se com este trabalho avaliar os efeitos que diferentes programas de restrição alimentar têm sobre matrizes de corte. Foram utilizadas 832 matrizes pesadas Cobb 500® com 31 dias distribuídas em 32 boxes, contendo 26 aves cada, seguindo um delineamento em blocos casualizados com 4 tratamentos (T1 – (controle) restrição diária, T2 - restrição “skip-a-day”, T3 - restrição 4/3 e T4 - r...

Caracterização de Requeijão Marajoara e Minas Frescal produzidos com leite de búfalas no Estado do Pará, Brasil

Directory of Open Access Journals (Sweden)

Ruth Helena Falesi Palha de Moraes Bittencourt

2013-09-01

Full Text Available Trinta amostras de queijo tipo Requeijão Marajoara e trinta de Minas Frescal, elaborados com leite bubalino foram analisadas, visando a caracterizar os teores dos principais componentes e características físico-químicas. Os queijos foram obtidos de estabelecimentos industriais localizados nos municípios de Salvaterra, Ilha de Marajó e Moju, nordeste do Estado do Pará. Os dados obtidos foram analisados por meio de Análise Descritiva, utilizando o pacote estatístico SAS®. Para o Requeijão Marajoara, os resultados médios foram: pH de 5,15 (±0,13; acidez 0,62% (±0,08; umidade 43% (±1,64; gordura 31,57% (±3,13; gordura no extrato seco (GES 55,84% (±4,38; proteína 34,59% (±1,68; e cinzas 2,45% (±0,47. Para o Minas Frescal, o pH foi de 6,26 (±0,69, acidez 0,15% (±0,14, umidade 60,99% (±2,58, gordura 25,09% (±1,68, GES 62,95% (±4,38, proteína 11,06% (±1,65 e as cinzas 2,09% (±0,32. A variação entre os macronutrientes e as características físico-químicas nas amostras estudadas revelou falta de padronização na tecnologia empregada no processamento, o que pode estar relacionada à inexistência de legislações específicas para a elaboração desses derivados com leite bubalino. Conclui-se que é fundamental o estabelecimento de critérios de processamento e requisitos legais, objetivando a obtenção de produtos padronizados, com consequente agregação de valor.

Temporal associations between national outbreaks of meningococcal serogroup W and C disease in the Netherlands and England: an observational cohort study.

Science.gov (United States)

Knol, Mirjam J; Hahné, Susan J M; Lucidarme, Jay; Campbell, Helen; de Melker, Hester E; Gray, Stephen J; Borrow, Ray; Ladhani, Shamez N; Ramsay, Mary E; van der Ende, Arie

2017-10-01

Since 2009, the incidence of meningococcal serogroup W disease has increased rapidly in the UK because of a single strain (the so-called original UK strain) belonging to the hypervirulent sequence type-11 clonal complex (cc11), with a variant outbreak strain (the so-called 2013 strain) emerging in 2013. Subsequently, the Netherlands has had an increase in the incidence of meningococcal serogroup W disease. We assessed the temporal and phylogenetic associations between the serogroup W outbreaks in the Netherlands and England, and the historical serogroup C outbreaks in both countries. For this observational cohort study, we used national surveillance data for meningococcal serogroup W and serogroup C disease in the Netherlands and England for the epidemiological years (July to June) 1992-93 to 2015-16. We also did whole genome sequencing and core genome multilocus sequence typing (1546 loci) on serogroup W disease isolates from both countries for surveillance years 2008-09 to 2015-16. We used Poisson regression to compare the annual relative increase in the incidence of serogroup W and serogroup C between both countries. In the Netherlands, the incidence of meningococcal serogroup W disease increased substantially in 2015-16 compared with 2014-15, with an incidence rate ratio of 5·2 (95% CI 2·0-13·5) and 11% case fatality. In England, the incidence increased substantially in 2012-13 compared with 2011-12, with an incidence rate ratio of 1·8 (1·2-2·8). The relative increase in the Netherlands from 2014-15 to 2015-16 was 418% (95% CI 99-1248), which was significantly higher than the annual relative increase of 79% (61-99) per year in England from 2011-12 to 2014-15 (p=0·03). Cases due to meningococcal serogroup W cc11 (MenW:cc11) emerged in 2012-13 in the Netherlands. Of 29 MenW:cc11 cases found up to 2015-16, 26 (90%) were caused by the 2013 strain. For both the current serogroup W outbreak and the historical serogroup C outbreak, the increase in incidence

Avaliação do papel do sistema canabidiol em um modelo de lesão renal por isquemia/reperfusão em animais

Directory of Open Access Journals (Sweden)

Rodrigo Zon Soares

2015-12-01

Full Text Available RESUMO Objetivo: Investigar os efeitos da administração de canabidiol em um modelo de isquemia/reperfusão renal em animais. Métodos: Foi induzida uma lesão renal, por meio de 45 minutos de isquemia renal seguida por reperfusão. Administrou-se canabidiol (5mg/kg imediatamente após a reperfusão. Resultados: A isquemia/reperfusão aumentou os níveis de interleucina 1 e fator de necrose tumoral, o que foi atenuado pelo tratamento com canabidiol. Além disso, o canabidiol foi capaz de diminuir o dano oxidativo de lipídios e proteínas, mas não os níveis de nitrito/nitrato. A lesão renal após isquemia/reperfusão pareceu ser independente da expressão dos receptores canabidiol-1 e canabidiol-2, já que não houve aumento significante desses receptores após a reperfusão. Conclusão: O tratamento com canabidiol teve um efeito protetor contra a inflamação e o dano oxidativo em um modelo de isquemia/reperfusão renal. Esses efeitos parecem não ocorrer via ativação dos receptores canabidiol-1/canabidiol-2.

Fractal formation of a Y-Ba-Cu-O thin film on SrTiO3

International Nuclear Information System (INIS)

Chow, L.; Chen, J.; Desai, V.; Sundaram, K.; Arora, S.

1989-01-01

Fractal formation has been observed after thermal annealing of the rf-sputtered Y-Ba-Cu-O thin film on SrTiO 3 substrate. Through energy-dispersive x-ray analysis, it was found that the composition of the fractal was YBa 2 Cu 3 O x and the surrounding film composition wasY 2 Ba 2 Cu 3 O x . The fractal dimensions D ranging from 1.26 to 1.65 were obtained using the standard sandbox method with different thresholds

Lunar mare TiO2 abundances estimated from UV/Vis reflectance

Science.gov (United States)

Sato, Hiroyuki; Robinson, Mark S.; Lawrence, Samuel J.; Denevi, Brett W.; Hapke, Bruce; Jolliff, Bradley L.; Hiesinger, Harald

2017-11-01

The visible (400-700 nm) and near-infrared (700-2800 nm) reflectance of the lunar regolith is dominantly controlled by variations in the abundance of plagioclase, iron-bearing silicate minerals, opaque minerals (e.g., ilmenite), and maturation products (e.g., agglutinate glass, radiation-produced rims on soil grains, and Fe-metal). The same materials control reflectance into the near-UV (250-400 nm) with varying degrees of importance. A key difference is that while ilmenite is spectrally neutral in the visible to near-infrared, it exhibits a diagnostic upturn in reflectance in the near-UV, at wavelengths shorter than about 450 nm. The Lunar Reconnaissance Orbiter Wide Angle Camera (WAC) filters were specifically designed to take advantage of this spectral feature to enable more accurate mapping of ilmenite within mare soils than previously possible. Using the reflectance measured at 321 and 415 nm during 62 months of repeated near-global WAC observations, first we found a linear correlation between the TiO2 contents of the lunar soil samples and the 321/415 nm ratio of each sample return site. We then used the coefficients from the linear regression and the near-global WAC multispectral mosaic to derive a new TiO2 map. The average TiO2 content is 3.9 wt% for the 17 major maria. The highest TiO2 values were found in Mare Tranquillitatis (∼12.6 wt%) and Oceanus Procellarum (∼11.6 wt%). Regions contaminated by highland ejecta, lunar swirls, and the low-TiO2 maria (e.g., Mare Frigoris, the northeastern units of Mare Imbrium) exhibit very low TiO2 values (2.6 Ga), whereas only medium to high TiO2 values (average = 6.8 wt%, minimum = 4.5 wt%) are found for younger mare units (<2.6 Ga).

Componentes não-integrantes da carcaça de novilhos não-castrados ou castrados terminados em confinamento e abatidos aos 16 ou 26 meses de idade Non-carcass components of castrate and non-castrated cattle finished in feedlot and slaughtered at 16 or 26 months of age

Directory of Open Access Journals (Sweden)

Fernando Kuss

2008-10-01

Full Text Available Foram avaliados os componentes não-integrantes da carcaça de novilhos terminados em confinamento e abatidos aos 16 (superjovem ou 26 (jovem meses de idade. A dieta foi formulada com 50% de volumoso e 50% de concentrado e continha 11,8% de proteína bruta e 2,83 Mcal de energia digestível por kg de matéria seca. Animais superjovens apresentaram maior rendimento de corpo vazio (92,39 versus 89,76% para os jovens, como resultado de seu menor conteúdo gastrintestinal (35,23 versus 53,46 kg para os jovens. Animais não-castrados apresentaram maior peso de cabeça (13,84 versus 12,35 kg, patas (11,12 versus 8,96 kg e couro (46,44 versus 37,71 kg em comparação aos castrados, o que está relacionado ao seu maior peso corporal (541,26 versus 445,47 kg. Observou-se influência da interação categoria x condição sexual sobre o peso absoluto dos órgãos vitais (coração, fígado e pulmões e dos componentes do trato gastrintestinal. O peso total de órgãos vitais e do trato gastrintestinal foi maior nos animais não-castrados, mas deixou de ser significativo quando ajustado para peso de corpo vazio (PCV e de abate (PA. Animais superjovens apresentaram maior peso absoluto das gorduras interna (25,91 versus 20,13 kg e de toalete (13,96 versus 10,98 kg. A castração dos animais resultou em maior participação de gordura interna calculada em relação ao peso de corpo vazio e ao peso de abate.The non-carcass components of castrated and non-castrated cattle (sex condition finished in feedlot and slaughtered at 16 (super young or 26 (young months of age (animal category were evaluated. The diet was formulated to contain 11.8% of CP and 2.83 Mcal/kg DM of DE with 50:50 forage to concentrate ratio (%MS. Super young animals showed higher of empty body dressing percentage (92.39 versus 89.76%, as a result of their lower gastrointestinal content (35.23 versus 53.46 kg as compared to the young animals. Non-castrate animals showed higher head weight (13

Elastic scattering of 16O on 28Si between 45.0 and 73.5 MeV

International Nuclear Information System (INIS)

Shkolnik, V.; Dehnhard, D.; Kubono, S.; Franey, M.A.; Tripp, S.; Artz, J.L.; Weber, D.J.

1977-01-01

Angular distributions of 16 O elastically scattering by Si isotopes were analyzed by the optical model. Differential cross sections are shown for all isotopes at 60 MeV, and for 28 Si at energies from 45 to 63 MeV. The potentials found are discussed in some detail; the best fits were produced by surface-transparent potentials. 3 figures, 2 tables

Efficient Inverted ITO-Free Organic Solar Cells Based on Transparent Silver Electrode with Aqueous Solution-Processed ZnO Interlayer

Directory of Open Access Journals (Sweden)

Zhizhe Wang

2017-01-01

Full Text Available Efficient inverted organic solar cells (OSCs with the MoO3 (2 nm/Ag (12 nm transparent cathode and an aqueous solution ZnO electron extraction layer processed at low temperature are investigated in this work. The blend of low bandgap poly[[4,8-bis[(2-ethylhexyloxy]benzo[1,2-b:4,5-b′]dithiophene-2,6-diyl][3-fluoro-2-[(2-ethylhexylcarbonyl]thieno[3,4-b]thiophenediyl

Efeito de diferentes níveis de ingestão de energia sobre a produção e viabilidade de embriões em novilhas e vacas Effect of different levels of energy intake on production and viability of embryos in heifers and cows

Directory of Open Access Journals (Sweden)

Luiz Paulo Rigolon

2003-12-01

Full Text Available O objetivo do trabalho foi estudar o efeito do nível de ingestão de matéria seca (IMS/% do PV sobre ganho de peso, produção e viabilidade de embriões em novilhas mestiças e vacas Nelore. No primeiro experimento foram utilizadas 27 novilhas cruzadas ( ½ Nelore x ½ Simental distribuídas em três tratamentos e nove repetições: 1,2; 1,6 e 2,6% de IMS/dia em relação ao peso vivo, respectivamente. Os animais foram submetidas à superovulação com FSH e a coleta dos embriões realizada sete dias após a inseminação artificial, através de lavagem uterina. Este procedimento foi repetido por mais duas vezes com intervalos de 45 dias. No segundo experimento foram usadas 21 vacas Nelore de três anos, distribuídas em três tratamentos: 1,0; 1,8 e 2,6% de IMS em relação ao peso vivo. Após 30 dias os animais foram sincronizados com implante auricular de norgestomet e superovulados com FSH, sendo a coleta dos embriões realizada sete dias após a inseminação artificial, através de lavagem uterina. O peso vivo final, ganho médio diário e peso de carcaça foram maiores para os animais alimentados com 2,6% de IMS. O nível de IMS e o período de coleta não tiveram influência sobre o número de corpos lúteos, número de estruturas totais transferíveis e degeneradas e ovócitos, tanto para novilhas como para vacas.This work was carried out to study the effect of dry matter feed intake level (DMI/% BW, weight gain, production and viability of embryos in cross-breed heifers so does production and viability of embryonic structures in Nellore cows. During the first trial, 27 cross-breed (½ Nelore x ½ Simental heifers were used divided in 3 treatments and 9 replications: 1.2%; 1.6% and 2.6% of DMI/day in relation to body weight, respectively. The heifers were synchronized and after nine days these animals were superovulated using FSH. The embryos were collected seven days after the artificial insemination by uterus flushing. This

Four Sporadic Pediatric Cases of Yersinia enterocolitica O:8 Infection in a Rural Area of Japan.

Science.gov (United States)

Minami, Kisei; Yasuda, Ryu; Terakawa, Runa; Koike, Yumi; Takeuchi, Koichi; Higuchi, Tsukasa; Horiuchi, Ayaka; Kubota, Noriko; Hidaka, Eiko; Kawakami, Yoshiyuki

2017-03-24

In the spring of 2015, we experienced a cluster of 4 sporadic cases of yersiniosis in children in Nagano prefecture, a rural area of Japan. Two patients developed appendicitis-like episodes; one had acute gastroenteritis, and the other had bacteremia associated with liver abscess. The causative agent of these infections was Yersinia enterocolitica serogroup O:8. None of the patients had an underlying illness, and all have recovered completely. The patients were neither socially nor geographically related to each other. These 4 consecutive cases suggest that Y. enterocolitica O:8 has spread substantially in the middle part of Japan, and that this virulent strain might be more common than previously reported in our country.

High-Performance 2.6 V Aqueous Asymmetric Supercapacitors based on In Situ Formed Na0.5 MnO2 Nanosheet Assembled Nanowall Arrays.

Science.gov (United States)

Jabeen, Nawishta; Hussain, Ahmad; Xia, Qiuying; Sun, Shuo; Zhu, Junwu; Xia, Hui

2017-08-01

The voltage limit for aqueous asymmetric supercapacitors is usually 2 V, which impedes further improvement in energy density. Here, high Na content Birnessite Na 0.5 MnO 2 nanosheet assembled nanowall arrays are in situ formed on carbon cloth via electrochemical oxidation. It is interesting to find that the electrode potential window for Na 0.5 MnO 2 nanowall arrays can be extended to 0-1.3 V (vs Ag/AgCl) with significantly increased specific capacitance up to 366 F g -1 . The extended potential window for the Na 0.5 MnO 2 electrode provides the opportunity to further increase the cell voltage of aqueous asymmetric supercapacitors beyond 2 V. To construct the asymmetric supercapacitor, carbon-coated Fe 3 O 4 nanorod arrays are synthesized as the anode and can stably work in a negative potential window of -1.3 to 0 V (vs Ag/AgCl). For the first time, a 2.6 V aqueous asymmetric supercapacitor is demonstrated by using Na 0.5 MnO 2 nanowall arrays as the cathode and carbon-coated Fe 3 O 4 nanorod arrays as the anode. In particular, the 2.6 V Na 0.5 MnO 2 //Fe 3 O 4 @C asymmetric supercapacitor exhibits a large energy density of up to 81 Wh kg -1 as well as excellent rate capability and cycle performance, outperforming previously reported MnO 2 -based supercapacitors. This work provides new opportunities for developing high-voltage aqueous asymmetric supercapacitors with further increased energy density. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Epidemiology of serogroup B invasive meningococcal disease in Ontario, Canada, 2000 to 2010

Directory of Open Access Journals (Sweden)

Dang Vica

2012-08-01

Full Text Available Abstract Background Invasive meningococcal disease (IMD caused by serogroup B is the last major serogroup in Canada to become vaccine-preventable. The anticipated availability of vaccines targeting this serogroup prompted an assessment of the epidemiology of serogroup B disease in Ontario, Canada. Methods We retrieved information on confirmed IMD cases reported to Ontario’s reportable disease database between January 1, 2000 and December 31, 2010 and probabilistically-linked these cases to Public Health Ontario Laboratory records. Rates were calculated with denominator data obtained from Statistics Canada. We calculated a crude number needed to vaccinate using the inverse of the infant ( Results A total of 259 serogroup B IMD cases were identified in Ontario over the 11-year period. Serogroup B was the most common cause of IMD. Incidence ranged from 0.11 to 0.27/100,000/year, and fluctuated over time. Cases ranged in age from 13 days to 101 years; 21.4% occurred in infants, of which 72.7% were Conclusions Although rare, the proportion of IMD caused by serogroup B has increased and currently causes most IMD in Ontario, with infants having the highest risk of disease. Although serogroup B meningococcal vaccines are highly anticipated, our findings suggest that decisions regarding publicly funding serogroup B meningococcal vaccines will be difficult and may not be based on disease burden alone.

Características das fibras musculares de cordeiros nascidos de ovelhas recebendo suplementação proteica no terço inicial da gestação Characteristics of muscle fibers of lambs born from sheep given protein suplementation in the intial third of pregnancy

Directory of Open Access Journals (Sweden)

Graziela Aparecida Santello

2010-10-01

Full Text Available Foram utilizadas 80 ovelhas Santa Inês, com 47,49 ± 3,76 kg de peso vivo, cobertas com reprodutores Dorper e alimentadas com concentrado com 15,20; 22,48; 26,52 e 35,65% de proteína bruta (PB no terço inicial de gestação. As ovelhas, durante o dia, permaneceram em pastagem de capim-aruana (Panicum maximum cv. Aruana. Ao nascimento, foram realizadas biópsias no músculo Semitendinosus esquerdo de cordeiros machos, para avaliar o número e o diâmetro das fibras musculares. Na fase de terminação, os cordeiros foram distribuídos em dois grupos e alimentados com uma dieta controle, contendo 16,18% de proteína bruta (PB e 79,64% nutrientes digestíveis totais (NDT, sem grão de girassol; ou com uma dieta contendo 9,10% de grãos de girassol (15,03% de PB e 78,96% de NDT. Não houve efeito da alimentação materna sobre o peso dos cordeiros ao nascimento (4,82 kg, aos 30 dias de idade (9,69 kg e ao desmame (15,38 kg. O sexo influenciou o peso dos cordeiros ao nascimento e ao desmame. O desempenho dos cordeiros na fase de terminação não foi influenciado pela dieta fornecida nessa fase, nem pelo sexo nem pelo manejo alimentar da mãe durante a gestação. Não houve diferença no número (2813,45 nem no diâmetro (13,16 μm das fibras musculares ao nascimento. O teor de PB de dietas para ovelhas no terço inicial da gestação pode ser reduzido, pois essa redução não influencia no diâmetro e número de fibras musculares.It was used 80 Santa Inês ewes with average body weight 47.79 ± 3.76 kg, mated with Dorper rams and fed concentrated diet with 15.20; 22.48; 26.52 and 35.65% crude protein (CP on the initial third of pregnancy. Sheep were kept on aruana grass (Panicum maximum cv. Aruana pasture during the day. At birth, biopsy on the left Semitendinosus muscle were done on male lambs to evaluate the number and diameter of muscular fibers. During finishing phase, lambs were distributed in two groups and fed control diet with 16

ESTUDO DA COBERTURA VEGETAL/USO DA TERRA NOS ANOS DE 1987 E 2007 NO NÚCLEO DE DEGRADAÇÃO/DESERTIFICAÇÃO DE SÃO RAIMUNDO NONATO - PIAUÍ

OpenAIRE

Cláudia Maria Sabóia de AQUINO; José Antonio Pacheco de ALMEIDA; José Gerardo Beserra de OLIVEIRA

2012-01-01

A cobertura vegetal é um componente de grande relevância para o equilíbrio ambiental, uma vez que assegura a proteção dos solos contra a erosão hídrica. Esta é recomendada pela ONU como um indicador para fins de avaliação do processo de desertificação. Considerando que aproximadamente 45,3% do estado do Piauí apresentam suscetibilidade a desertificação, este trabalho objetivou analisar de forma espaço-temporal, com o emprego de técnicas de sensoriamento remoto (detecção de mudanças), a dinâmi...

Uptake of TiO2 Nanoparticles into C. elegans Neurons Negatively Affects Axonal Growth and Worm Locomotion Behavior.

Science.gov (United States)

Hu, Chun-Chih; Wu, Gong-Her; Hua, Tzu-En; Wagner, Oliver I; Yen, Ta-Jen

2018-03-14

We employ model organism Caenorhabditis elegans to effectively study the toxicology of anatase and rutile phase titanium dioxide (TiO 2 ) nanoparticles (NPs). The experimental results show that nematode C. elegans can take up fluorescein isothiocyanate-labeled TiO 2 NPs and that both anatase and rutile TiO 2 NPs can be detected in the cytoplasm of cultured primary neurons imaged by transmission electron microscopy. After TiO 2 NP exposure, these neurons also grow shorter axons, which may be related to the detected impeded worm locomotion behavior. Furthermore, anatase TiO 2 NPs did not affect the worm's body length; however, we determined that a concentration of 500 μg/mL of anatase TiO 2 NPs reduced the worm population by 50% within 72 h. Notably, rutile TiO 2 NPs negatively affect both the body size and worm population. Worms unable to enter the L4 larval stage explain a severe reduction in the worm population at TiO 2 NPs LC 50 /3d. To obtain a better understanding of the cellular mechanisms involved in TiO 2 NP intoxication, DNA microarray assays were employed to determine changes in gene expression in the presence or absence of TiO 2 NP exposure. Our data reveal that three genes (with significant changes in expression levels) were related to metal binding or metal detoxification (mtl-2, C45B2.2, and nhr-247), six genes were involved in fertility and reproduction (mtl-2, F26F2.3, ZK970.7, clec-70, K08C9.7, and C38C3.7), four genes were involved in worm growth and body morphogenesis (mtl-2, F26F2.3, C38C3.7, and nhr-247), and five genes were involved in neuronal function (C41G6.13, C45B2.2, srr-6, K08C9.7, and C38C3.7).

Photocatalytic degradation of Acephate, Omethoate, and Methyl parathion by Fe{sub 3}O{sub 4}@SiO{sub 2}@mTiO{sub 2} nanomicrospheres

Energy Technology Data Exchange (ETDEWEB)

Zheng, Lingling, E-mail: zllyslngj@126.com [State Key Laboratory of Food Science and Technology, School of Food Science of Jiangnan University, School of Food Science Synergetic Innovation Center of Food Safety and Quality Control, Wuxi, Jiangsu 214122 (China); Pi, Fuwei; Wang, Yifan [State Key Laboratory of Food Science and Technology, School of Food Science of Jiangnan University, School of Food Science Synergetic Innovation Center of Food Safety and Quality Control, Wuxi, Jiangsu 214122 (China); Xu, Hui [Key Laboratory of Healthy & Intelligent Kitchen System Integration, Ningbo, Zhejiang 315336 (China); Zhang, Yinzhi [State Key Laboratory of Food Science and Technology, School of Food Science of Jiangnan University, School of Food Science Synergetic Innovation Center of Food Safety and Quality Control, Wuxi, Jiangsu 214122 (China); Sun, Xiulan, E-mail: sxlzzz@jiangnan.edu.cn [State Key Laboratory of Food Science and Technology, School of Food Science of Jiangnan University, School of Food Science Synergetic Innovation Center of Food Safety and Quality Control, Wuxi, Jiangsu 214122 (China)

2016-09-05

Highlights: • An efficient photocatalyst Fe{sub 3}O{sub 4}@SiO{sub 2}@mTiO{sub 2} with high magnetic response and large specific surface area was synthesized. • Photocatalytic efficiency of Fe{sub 3}O{sub 4}@SiO{sub 2}@mTiO{sub 2} on Acephate, Omethoate, and Methyl Parathion was higher than TiO{sub 2} P-25. • Possible photocatalytic degradation mechanisms for the Acephate, Omethoate, and Methyl Parathion were proposed. - Abstract: A novel magnetic mesoporous nanomicrospheres Fe{sub 3}O{sub 4}@SiO{sub 2}@mTiO{sub 2} were synthetized and characterized by a series of techniques including FE-TEM, EDS, FE-SEM, PXRD, XPS, BET, TGA as well as VSM, and subsequently tested as a photocatalyst for the degradation of Acephate, Omethoate, and Methyl parathion under UV irradiation. The well-designed nanomicrospheres exhibit a pure and highly crystalline anatase TiO{sub 2} layer, large specific surface area, and high-magnetic-response. Photocatalytic degradation of the three organophosphorus pesticides (OPPs) and the formation intermediates were identified using HPLC, TOC-V{sub cpn}, IC, pH meter and GC–MS. Acephate, Omethoate, and Methyl parathion disappeared after 45 min, 45 min, and 80 min UV illumination, respectively. At the end of the treatment, the total organic carbon (TOC) of the OPPs was reduced 80–85%. The main mineralization products were SO{sub 4}{sup 2−}, NO{sub 3}{sup −} and PO{sub 4}{sup 3−} and Omethoate additionally formed NO{sub 2}{sup −}. Based on the results, we proposed the photocatalytic degradation pathways for Acephate, Omethoate, and Methyl parathion.

Alteração da temperatura radicular externa durante o preparo para contenção intra-radicular

Directory of Open Access Journals (Sweden)

FERREIRA Cláudio Maníglia

1999-01-01

Full Text Available O objetivo deste trabalho foi comparar in vitro a alteração de temperatura na superfície radicular externa durante o preparo para contenção intra-radicular utilizando 4 técnicas de remoção da guta-percha: condensadores aquecidos, brocas de Peeso, Gates-Glidden e Largo, em 40 dentes pré-molares inferiores (unirradiculares tratados endodonticamente. O experimento foi realizado em uma câmara com temperatura controlada em torno de 26°C. Para a remoção da guta-percha do interior dos canais, foram calculados dois terços do comprimento da raiz, deixando em torno de 4 mm de obturação apical. As medições da temperatura radicular externa foram realizadas com um termopar acoplado a um multímetro e efetuadas em 3 regiões: cervical (em torno de 3 mm abaixo da junção cemento-esmalte, média e apical. A análise de variância indicou diferenças significantes (p < 0,01 entre as regiões e as técnicas utilizadas. O teste complementar de Tukey indicou que os condensadores aquecidos produziram o maior aumento de temperatura dentre as técnicas, e a broca de Peeso dentre as brocas, seguida pela de Largo e de Gates-Glidden. Dentre as regiões, a apical apresentou a maior alteração de temperatura em todas as técnicas avaliadas.

Emerging types of Shiga toxin-producing E. coli (STEC O178 present in cattle, deer and humans from Argentina and Germany

Directory of Open Access Journals (Sweden)

Angelika eMiko

2014-06-01

Full Text Available More than 400 serotypes of Shiga toxin-producing Escherichia coli (STEC have been implicated in outbreaks and sporadic human diseases. In recent years STEC strains belonging to serogroup O178 have been commonly isolated from cattle and food of bovine origin in South America and Europe. In order to explore the significance of these STEC strains as potential human pathogens, 74 German and Argentinean E. coli O178 strains from animals, food and humans were characterized phenotypically and investigated for their serotypes, stx-genotypes and forty-three virulence-associated markers by a real-time PCR-microarray. The majority (n=66 of the O178 strains belonged to serotype O178:H19. The remaining strains divided into O178:H7 (n=6, O178:H10 (n=1 and O178:H16 (n=1. STEC O178:H19 strains were mainly isolated from cattle and food of bovine origin, but one strain was from a patient with hemolytic uremic syndrome (HUS. Genotyping of the STEC O178:H19 strains by pulsed-field gel electrophoresis revealed two major clusters of genetically highly related strains which differ in their stx-genotypes and non-Stx putative virulence traits, including adhesins, toxins and serine-proteases. Cluster A-strains including the HUS-strain (n=35 carried genes associated with severe disease in humans (stx2a, stx2d, ehxA, saa, subAB1, lpfAO113, terE combined with stx1a, espP, iha. Cluster B-strains (n=26 showed a limited repertoire of virulence genes (stx2c, pagC, lpfAO113, espP, iha. Among O178:H7 strains isolated from deer meat and patients with uncomplicated disease a new STEC variant was detected that is associated with the genotype stx1c/stx2b/ehxA/subAB2/espI/[terE]/espP/iha. None of the STEC O178 strains was positive for locus of enterocyte effacement (LEE- and nle-genes. Results indicate that STEC O178:H19 strains belong to the growing group of LEE-negative STEC that should be considered with respect to their potential to cause diseases in humans.

Analysis on the energetics, magnetism and electronic properties in a 45° ZnO grain boundary doped with Gd

KAUST Repository

Devi, Assa Aravindh Sasikala; Roqan, Iman S.

2018-01-01

The structural stability and magnetic properties of a grain boundary (GB) formed by aligning two ZnO single crystals oriented at an angle of 45° is investigated by density functional theory, using generalized gradient approximation (GGA) and taking

Crystal structure of bis-(μ-3-nitro-benzoato)-κ3O,O':O;κ3O:O,O'-bis-[bis-(3-cyano-pyridine-κN1)(3-nitro-benzoato-κ2O,O')cadmium].

Science.gov (United States)

Hökelek, Tuncer; Akduran, Nurcan; Özen, Azer; Uğurlu, Güventürk; Necefoğlu, Hacali

2017-03-01

The asymmetric unit of the title compound, [Cd 2 (C 7 H 4 NO 4 ) 4 (C 6 H 4 N 2 ) 4 ], contains one Cd II atom, two 3-nitro-benzoate (NB) anions and two 3-cyano-pyridine (CPy) ligands. The two CPy ligands act as monodentate N(pyridine)-bonding ligands, while the two NB anions act as bidentate ligands through the carboxyl-ate O atoms. The centrosymmetric dinuclear complex is generated by application of inversion symmetry, whereby the Cd II atoms are bridged by the carboxyl-ate O atoms of two symmetry-related NB anions, thus completing the distorted N 2 O 5 penta-gonal-bipyramidal coordination sphere of each Cd II atom. The benzene and pyridine rings are oriented at dihedral angles of 10.02 (7) and 5.76 (9)°, respectively. In the crystal, C-H⋯N hydrogen bonds link the mol-ecules, enclosing R 2 2 (26) ring motifs, in which they are further linked via C-H⋯O hydrogen bonds, resulting in a three-dimensional network. In addition, π-π stacking inter-actions between parallel benzene rings and between parallel pyridine rings of adjacent mol-ecules [shortest centroid-to-centroid distances = 3.885 (1) and 3.712 (1) Å, respectively], as well as a weak C-H⋯π inter-action, may further stabilize the crystal structure.

Investigation of gamma ray shielding, structural and dissolution rate properties of Bi2O3-BaO-B2O3-Na2O glass system

Science.gov (United States)

Dogra, Mridula; Singh, K. J.; Kaur, Kulwinder; Anand, Vikas; Kaur, Parminder; Singh, Prabhjot; Bajwa, B. S.

2018-03-01

In the present study, quaternary system of the composition (0.45 + x) Bi2O3-(0.25 - x) BaO-0.15 B2O3-0.15 Na2O (where 0 ≤ x ≤ 0.2 mol fraction) has been prepared by using melt-quenching technique for investigation of gamma ray shielding properties. Mass attenuation coefficients and half value layer parameters have been determined experimentally at 662 keV by using 137Cs source. It has been found that experimental results of these parameters hold good agreement with theoretical values. The density, molar volume, XRD, FTIR, Raman and UV-visible studies have been used to determine structural properties of the prepared glass samples. Dissolution rate of the samples has also been measured to check their utility as long term durable glasses.

Excesso de ferro sobre o crescimento e a composição mineral em Eugenia uniflora L.

Directory of Open Access Journals (Sweden)

Gládis de Oliveira Jucoski

2016-12-01

Full Text Available RESUMO O ferro, embora micronutriente essencial, quando em excesso pode causar redução no crescimento e, consequentemente, na produtividade das plantas. O objetivo do presente trabalho foi avaliar a absorção, o acúmulo, a distribuição do Fe e suas consequências sobre o crescimento e composição mineral de plantas jovens de Eugenia uniflora L.. As plantas foram expostas às concentrações de Fe 0,045 (controle, 1,0 e 2,0 mM, aplicado na forma de FeEDTA, em solução nutritiva de Hoagland, pH 5,0, durante 15; 30 e 45 dias e, então, foram avaliados o número de folhas, a altura da parte aérea, o comprimento da raiz primária, a massa seca de raízes, caule e folhas e os teores de clorofila, carotenóides totais e minerais. Plantas expostas aos tratamentos com Fe 1,0 e 2,0 mM apresentaram aumento nos teores deste elemento em folhas, caule e raízes em relação às plantas-controle, especialmente aos 45 dias de exposição. Sob esta condição, as plantas exibiram sintomatologia típica de toxidez de Fe, caracterizada por bronzeamento foliar, escurecimento das raízes, redução no número de folhas, na altura da parte aérea, no comprimento da raiz principal, na produção de massa seca e nos teores de pigmentos cloroplastídicos. O excesso de Fe modificou a partição da biomassa e promoveu redução nos teores de P, Zn, Cu e Mn, especialmente nas raízes. Além de um efeito direto do Fe em excesso, a desordem nutricional resultante pode estar associada aos efeitos restritivos sobre o crescimento vegetativo inicial das plantas de Eugenia uniflora L..

Tumor de colisão de pele do pescoço

Directory of Open Access Journals (Sweden)

Helma Maria Chedid

Full Text Available O tumor de colisão ou tumor misto é uma neoplasia maligna de pele, relacionada à exposição solar e com índices de incidência de até 1,5%. Apresenta comportamento clínico peculiar, em relação às demais neoplasias malignas de pele e com diagnóstico histológico, caracterizado pela colisão entre um carcinoma basocelular e um carcinoma epidermóide, ou seja, duas neoplasias com histologias distintas e interface nítida entre ambas. O caso relatado foi de paciente do sexo masculino, 73 anos, com duas lesões cervicais de crescimento progressivo nos últimos meses. O tratamento realizado foi cirúrgico, com exame histológico demonstrando a presença de carcinoma de células escamosas contíguo ao carcinoma de células basais. O acometimento preferencial ocorre em homens de pele clara, na quinta ou sexta décadas de vida. Sua localização mais comum é na cabeça e pescoço, principalmente na parte central da face. O carcinoma basoescamoso é diagnóstico diferencial, definido através de critérios histológicos distintos, uma vez que ambas neoplasias apresentam comportamento clínico semelhante. Os índices de recidiva local variam de 12% a 45%, enquanto que é baixo na recidiva regional, de aproximadamente 7,5%. Os principais fatores prognósticos são o gênero do paciente, margens cirúrgicas, infiltração perineural e status linfonodal. O tratamento de escolha é a ressecção, sendo a radioterapia indicada na sua adjuvância e lesões irressecáveis. A recidiva local é o principal fator limitante na sobrevida livre de doença que apresenta resultados pobres.

Crystal structure of ethylenedioxytetrathiafulvalene-4,5-bis(thiolbenzoic acid 0.25-hydrate

Directory of Open Access Journals (Sweden)

Yuanyuan Zhang

2017-09-01

Full Text Available In the title compound (systematic name: 4,4′-{[2-(5,6-dihydro-[1,3]dithiolo[4,5-b][1,4]dioxin-2-ylidene-1,3-dithiole-4,5-diyl]bis(sulfanediyl}dibenzoic acid 0.25-hydrate, C22H14O6S6·0.25H2O, the tetrathiafulvalene (TTF core adopts a boat conformation, where the central S2C=CS2 plane makes dihedral angles of 31.34 (4 and 26.83 (6°, respectively, with the peripheral S2C=CS2 and S2C2O2 planes. In the crystal, the benzoic acid molecules are linked via O—H...O hydrogen bonds, forming inversion dimers with R22(8 motifs. The dimers are linked through weak C—H...O hydrogen bonds into a chain structure along [-101]. The chains stack along the a axis through S...S and S...C short contacts, forming layers parallel to the ac plane.

Photoelectrochemical oxidation of ibuprofen via Cu{sub 2}O-doped TiO{sub 2} nanotube arrays

Energy Technology Data Exchange (ETDEWEB)

Sun, Qiannan [College of Environment and Energy, South China University of Technology, Guangzhou (China); Peng, Yen-Ping, E-mail: yppeng@thu.edu.tw [Department of Environmental Science and Engineering, Tunghai University, Taichung, Taiwan (China); Chen, Hanlin [College of Environment and Energy, South China University of Technology, Guangzhou (China); Chang, Ken-Lin [School of Environmental Science and Engineering and Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou, 51006 China (China); Qiu, Yang-Neng; Lai, Shiau-Wu [Department of Environmental Science and Engineering, Tunghai University, Taichung, Taiwan (China)

2016-12-05

Highlights: • A p–n junction material was synthesized to enhance photocatalytic ability. • Cu{sub 2}O-doped TiO{sub 2} nanotube arrays works as a photoanode in a PEC system. • Recombination of photo-generated holes and electrons were greatly reduced. • Synergetic effect was quantified in PEC degradation. • Recombination of photogenerated holes and electrons was greatly enhanced. - Abstract: A p–n junction based Cu{sub 2}O-doped TiO{sub 2} nanotube arrays (Cu{sub 2}O-TNAs) were synthesized and used as a working anode in a photoelectrochemical (PEC) system. The results revealed that the Cu{sub 2}O-TNAs were dominated by the anatase phase and responded significantly to visible light. XPS analyses indicated that with an amount of 24.79% Cu doping into the structure, the band gap of Cu{sub 2}O-TNAs was greatly reduced. SEM images revealed that the supported TiO{sub 2} nanotubes had diameters of approximately 80 nm and lengths of about 2.63 μm. Upon doping with Cu{sub 2}O, the TiO{sub 2} nanotubes maintained their structural integrity, exhibiting no significant morphological change, favoring PEC applications. Under illumination, the photocurrent from Cu{sub 2}O/TNAs was 2.4 times larger than that from TNAs, implying that doping with Cu{sub 2}O significantly improved electron mobility by reducing the rate of recombination of electron-hole pairs. The EIS and Bode plot revealed that the estimated electron lifetimes, τ{sub el}, of TNAs and Cu{sub 2}O/TNAs were 6.91 and 26.26 ms, respectively. The efficiencies of degradation of Ibuprofen by photoelectrochemical, photocatalytic (PC), electrochemical (EC) and photolytic (P) methods were measured.

Detection of cell-associated or soluble antigens of Legionella pneumophila serogroups 1 to 6, Legionella bozemanii, Legionella dumoffii, Legionella gormanii, and Legionella micdadei by staphylococcal coagglutination tests.

OpenAIRE

Wilkinson, H W; Fikes, B J

1981-01-01

Current methods used for the detection of whole-cell isolates of Legionella or for the detection of Legionella soluble antigens are technically impractical for many clinical laboratories. The purpose of this study was to explore practical alternatives. The results showed that whole cell isolates of Legionella pneumophila serogroups 1 to 6, Legionella bozemanii, Legionella dumoffii, Legionella gormanii, and Legionella micdadei were identified specifically by a simple slide agglutination test o...

Microwave irradiated Ni–MnO{sub x}/C as an electrocatalyst for methanol oxidation in KOH solution for fuel cell application

Energy Technology Data Exchange (ETDEWEB)

Hameed, R.M. Abdel, E-mail: randa311eg@yahoo.com

2015-12-01

Graphical abstract: - Highlights: • Ni–MnO{sub x}/C had nickel nanoparticles with an average diameter of 4.5 nm. • Oxidation current density increased by 1.43 times at Ni–MnO{sub x}/C. • Ni–MnO{sub x}/C showed k{sub s} value of 3.26 × 103 cm{sup 3} mol{sup −1} s{sup −1}. • Adding MnO{sub x} to Ni/C lowered its phase angle and impedance values. - Abstract: Ni–MnO{sub x}/C electrocatalyst was synthesized by the reduction of nickel precursor salt on MnO{sub x}/C powder using NaBH{sub 4} and the deposition process was motivated with the aid of microwave irradiation. Finer nickel nanoparticles were detected in Ni–MnO{sub x}/C using transmission electron microscopy with a lower particle size of 4.5 nm compared to 6 nm in Ni/C. Cyclic voltammetry, chronoamperometry and electrochemical impedance spectroscopy (EIS) were applied to study the electrocatalytic activity of Ni–MnO{sub x}/C for methanol oxidation in 0.5 M KOH solution. The presence of 7.5 wt.% MnO{sub x} in Ni–MnO{sub x}/C enhanced the oxidation current density by 1.43 times. The catalytic rate constant of methanol oxidation at Ni–MnO{sub x}/C was calculated as 3.26 × 10{sup 3} cm{sup 3} mol{sup −1} s{sup −1}. An appreciable shift in the maximum frequency at the transition from the resistive to capacitive regions to a higher value in Bode plots of Ni–MnO{sub x}/C was shown when compared to Ni/C. It was accompanied by lowered phase angle values. The lowered Warburg impedance value (W) of Ni–MnO{sub x}/C at 400 mV confirmed the faster methanol diffusion rate at its surface.

Influência da irrigação intraperitoneal de solução salina em diferentes temperaturas na formação de aderências e prevenção de hipotermia após pneumoperitônio em ratos

Directory of Open Access Journals (Sweden)

Oswaldo Lourenço de Molla Neto

Full Text Available Este estudo experimental em ratos avalia a influência da irrigação da cavidade peritoneal com solução salina isotônica (0,9 %, em diferentes temperaturas, na formação de aderências peritoneais e prevenção de hipotermia após pneumoperitônio. Foram utilizados 80 ratos divididos em quatro grupos de 20 animais: grupo controle (G1 sem irrigação, grupos com irrigação a temperatura ambiente 22,0°C (G2, a 35,0°C (G3 e a 45,0°C (G4. A análise da hipotermia foi realizada através da monitorização da temperatura retal em três diferentes momentos: após a anestesia (T1, cinco minutos depois da insuflação de dióxido de carbono (T2 e cinco minutos após a irrigação com solução salina (T3. Os animais foram sacrificados no 28º dia de pós-operatório. Observaram-se aderências nos grupos com irrigação, sendo que, com salina à temperatura de 45,0ºC houve maior formação de aderências (30,0% , porém, esta diferença não foi significante. No G2 ocorreu uma queda significante na temperatura média retal quando comparada aos demais grupos, demonstrando que a hipotermia na cirurgia laparoscópica pode ser reduzida com o uso de solução salina aquecida.

Tris(2,2′-bipyridine-κ2N,N′cobalt(III bis[bis(pyridine-2,6-dicarboxylato-κ3O2,N,O6cobaltate(III] perchlorate dimethylformamide hemisolvate 1.3-hydrate

Directory of Open Access Journals (Sweden)

Irina A. Golenya

2012-10-01

Full Text Available In the title compound, [Co(C10H8N23][Co(C7H3NO42]2(ClO4·0.5C3H7NO·1.3H2O, the CoIII atom in the complex cation is pseudooctahedrally coordinated by six N atoms of three chelating bipyridine ligands. The CoIII atom in the complex anion is coordinated by two pyridine N atoms and four carboxylate O atoms of two doubly deprotonated pyridine-2,6-dicarboxylate ligands in a distorted octahedral geometry. One dimethylformamide solvent molecule and two water molecules are half-occupied and one water molecule is 0.3-occupied. O—H...O hydrogen bonds link the water molecules, the perchlorate anions and the complex anions. π–π interactions between the pyridine rings of the complex anions are also observed [centroid–centroid distance = 3.804 (3 Å].

Sputtered PdO Decorated TiO2 Sensing Layer for a Hydrogen Gas Sensor

Directory of Open Access Journals (Sweden)

Jeong Hoon Lee

2018-01-01

Full Text Available We report a sputtered PdO decorated TiO2 sensing layer by radiofrequency (RF sputtering methods and demonstrated gas sensing performance for H2 gas. We prepared sputtered anatase TiO2 sensing films with 200 nm thickness and deposited a Pd layer on top of the TiO2 films with a thickness ranging from 3 nm to 13 nm. Using an in situ TiO2/Pd multilayer annealing process at 550°C for 1 hour, we observed that Pd turns into PdO by Auger electron spectroscopy (AES depth profile and confirmed decorated PdO on TiO2 sensing layer from scanning electron microscope (SEM and atomic-force microscope (AFM. We also observed a positive sensing signal for 3, 4.5, and 6.5 nm PdO decorated TiO2 sensor while we observed negative output signal for a 13.5 nm PdO decorated one. Using a microheater platform, we acquired fast response time as ~11 sec and sensitivity as 6 μV/ppm for 3 nm PdO under 33 mW power.

Site selective, time and temperature dependent spectroscopy of Eu{sup 3+} doped apatites (Mg,Ca,Sr){sub 2}Y{sub 8}Si{sub 6}O{sub 26}

Energy Technology Data Exchange (ETDEWEB)

Jansen, T., E-mail: t.jansen@fh-muenster.de [Münster University of Applied Sciences, Stegerwaldstrasse 39, 48565 Steinfurt (Germany); Jüstel, T. [Münster University of Applied Sciences, Stegerwaldstrasse 39, 48565 Steinfurt (Germany); Kirm, M.; Mägi, H.; Nagirnyi, V.; Tõldsepp, E.; Vielhauer, S. [Institute of Physics, University of Tartu, W. Ostwald Str. 1, 50411 Tartu (Estonia); Khaidukov, N.M. [N. S. Kurnakov Institute of General and Inorganic Chemistry, 31 Leninskiy Prospekt, 119991 Moscow (Russian Federation); Makhov, V.N. [P.N. Lebedev Physical Institute, 53 Leninskiy Prospekt, 119991 Moscow (Russian Federation)

2017-06-15

This work concerns the optical properties of alkaline earth yttrium apatites according to the composition AE{sub 2}Y{sub 8}Si{sub 6}O{sub 26} (AE=Mg, Ca, Sr) doped with Eu{sup 3+}, which are materials of interest for LED applications. Using a multistep preparation route, which includes hydrothermal synthesis of precursors for solid state reaction, ceramic samples were prepared and their structural and optical properties characterised. More particularly, this work relates to site-selective spectroscopy, since the compounds comprise two distinguishable crystallographic sites within the host structure, where Eu{sup 3+} can be substituted. It also describes the temperature dependent photoluminescence, which thermal quenching temperature (T{sub 1/2}) for Sr{sub 2}Y{sub 8}Si{sub 6}O{sub 26}:Eu{sup 3+} and Ca{sub 2}Y{sub 8}Si{sub 6}O{sub 26}:Eu{sup 3+} is in the range of 561 K and 591 K respectively, whereas Mg{sub 2}Y{sub 8}Si{sub 6}O{sub 26}:Eu{sup 3+} shows bi-sigmoidal quenching behaviour in the range between 210 and 452 K.

Al2O3 Passivation Effect in HfO2·Al2O3 Laminate Structures Grown on InP Substrates.

Science.gov (United States)

Kang, Hang-Kyu; Kang, Yu-Seon; Kim, Dae-Kyoung; Baik, Min; Song, Jin-Dong; An, Youngseo; Kim, Hyoungsub; Cho, Mann-Ho

2017-05-24

The passivation effect of an Al 2 O 3 layer on the electrical properties was investigated in HfO 2 -Al 2 O 3 laminate structures grown on indium phosphide (InP) substrate by atomic-layer deposition. The chemical state obtained using high-resolution X-ray photoelectron spectroscopy showed that interfacial reactions were dependent on the presence of the Al 2 O 3 passivation layer and its sequence in the HfO 2 -Al 2 O 3 laminate structures. Because of the interfacial reaction, the Al 2 O 3 /HfO 2 /Al 2 O 3 structure showed the best electrical characteristics. The top Al 2 O 3 layer suppressed the interdiffusion of oxidizing species into the HfO 2 films, whereas the bottom Al 2 O 3 layer blocked the outdiffusion of In and P atoms. As a result, the formation of In-O bonds was more effectively suppressed in the Al 2 O 3 /HfO 2 /Al 2 O 3 /InP structure than that in the HfO 2 -on-InP system. Moreover, conductance data revealed that the Al 2 O 3 layer on InP reduces the midgap traps to 2.6 × 10 12 eV -1 cm -2 (compared to that of HfO 2 /InP, that is, 5.4 × 10 12 eV -1 cm -2 ). The suppression of gap states caused by the outdiffusion of In atoms significantly controls the degradation of capacitors caused by leakage current through the stacked oxide layers.

Comparação do padrão de floração e de visitação do meloeiro do tipo amarelo em Juazeiro-BA¹

Directory of Open Access Journals (Sweden)

Kátia Maria Medeiros de Siqueira

2011-10-01

Full Text Available O trabalho foi realizado em área experimental da UNEB, em Juazeiro-BA, durante os anos de 2010 e 2011, com o objetivo de descrever aspectos relativos à fenologia, à biologia floral e ao comportamento dos visitantes florais Apis mellifera do meloeiro do tipo amarelo, na região do Vale do São Francisco. Em novembro de 2010, registrou-se uma média de 22,7±2,6 dias de floração com emissão de flores exclusivamente masculinas até o segundo dia, e emissão de flores hermafroditas por 16 dias. Em maio de 2011, a média de dias de floração foi de 19,3±4,3, e com produção de flores exclusivamente masculinas até o quarto dia e emissão de flores hermafroditas por 20 dias. A média de flores por planta e a razão sexual foram de 96,2±4,2 e 1:16, em novembro de 2010, e de 67,1±17,7 e 1:19, em maio de 2011, respectivamente. As flores hermafroditas apresentaram maior volume de néctar do que as masculinas em ambos os períodos, sendo a causa provável do maior número de visitas recebidas por este tipo floral. O maior número de visitas ocorreu no período da manhã, com destaque para a coleta de pólen no horário das 7h às 11h.

Experience melting through the Earth's lower mantle via LH-DAC experiments on MgO-SiO2 and CaO-MgO-SiO2 systems

Science.gov (United States)

Baron, Marzena A.; Lord, Oliver T.; Walter, Michael J.; Trønnes, Reidar G.

2015-04-01

The large low shear-wave velocity provinces (LLSVPs) and ultra-low velocity zones (ULVZs) of the lowermost mantle [1] are likely characterized by distinct chemical compositions, combined with temperature anomalies. The heterogeneities may have originated by fractional crystallization of the magma ocean during the earliest history of the Earth [2,3] and/or the continued accretion at the CMB of subducted basaltic oceanic crust [4,5]. These structures and their properties control the distribution and magnitude of the heat flow at the CMB and therefore the convective dynamics and evolution of the whole Earth. To determine the properties of these structures and thus interpret the seismic results, a good understanding of the melting phase relations of relevant basaltic and peridotitic compositions are required throughout the mantle pressure range. The melting phase relations of lower mantle materials are only crudely known. Recent experiments on various natural peridotitic and basaltic compositions [6-8] have given wide ranges of solidus and liquidus temperatures at lower mantle pressures. The melting relations for MgO, MgSiO3 and compositions along the MgO-SiO2 join from ab initio theory [e.g. 9,10] is broadly consistent with a thermodynamic model for eutectic melt compositions through the lower mantle based on melting experiments in the MgO-SiO2 system at 16-26 GPa [3]. We have performed a systematic study of the melting phase relations of analogues for peridotitic mantle and subducted basaltic crust in simple binary and ternary systems that capture the major mineralogy of Earth's lower mantle, using the laser-heated diamond anvil cell (LH-DAC) technique at 25-100 GPa. We determined the eutectic melting temperatures involving the following liquidus mineral assemblages: 1. bridgmanite (bm) + periclase (pc) and bm + silica in the system MgO-SiO2 (MS), corresponding to model peridotite and basalt compositions 2. bm + pc + Ca-perovskite (cpv) and bm + silica + cpv in the

Airborne fungi in the region of Cubatão, São Paulo State, Brazil Fungos anemófilos da região de Cubatão, São Paulo, Brasil

Directory of Open Access Journals (Sweden)

Iracema H. Schoenlein-Crusius

2001-03-01

Full Text Available From March/1993 to March/1995, airborne fungi were isolated from the "Vale do Rio Moji" (highly affected by the air pollution caused by fertilizer industries, steel works, cement factories and chemical products industries, among others and from the "Vale do Rio Pilões" (less affected by the air pollution, both in the municipality of Cubatão, São Paulo State, Brazil, by exposing Petri dishes with potato dextrose agar medium, placed one meter from the ground, during 5 min. After 5 days of incubation at 20ºC, the colonies of the fungi were purified and identified, resulting in the identification of 39 taxa, three unidentified strains of Fusarium and non-sporulating cultures. A total of 28 taxa, one unidentified strain of Fusarium and non-sporulating fungi (71 records were isolated in the "Vale do Rio Moji" and 29 taxa, two unidentified strains of Fusarium and non-sporulating fungi (72 records in the "Vale do Rio Pilões. The airborne mycota of the higher polluted site presented 17 common, 12 rare and only one constant fungal species. In the other site, the airborne mycota was composed by 19 common, 10 rare and two constant fungal species. Among the obtained fungi, at least 12 taxa were reported as opportunistic fungi, 26 have been mentioned related to plant diseases and eight have been associated to allergy problems. The similarity Index of Sörensen between the mycotas, corresponding to 58%, may be considered low, and is probably justified by the air pollution, that mainly distinguish the two studied areas.De março/1993 a março/1995, foram isolados fungos anemófilos no Vale do Rio Moji (afetada pela poluição aérea causada por indústrias de fertilizantes, metalúrgicas, fábricas de cimento e indústrias de produtos químicos, entre outras e no Vale do Rio Pilões (área menos afetada pela poluição aérea, no município de Cubatão, Estado de São Paulo, Brasil, expondo placas-de-Petri, contendo meio de batata-dextrose-ágar, durante

Modelos de regressão multivariada empregando seleção de intervalos para a quantificação do biodiesel em blendas biodiesel/diesel

Directory of Open Access Journals (Sweden)

Marco Flôres Ferrão

2010-01-01

Full Text Available No presente trabalho foram analisados e comparados modelos de regressão multivariados por mínimos quadrados parciais porintervalo (iPLS e por mínimos quadrados parciais por exclusão (biPLS que selecionaram regiões do espectro mais adequadas,retirando informações não relevantes e otimizando o modelo de calibração, a fim de determinar a concentração de biodiesel emblendas de biodiesel/diesel a partir de dados obtidos por espectroscopia no infravermelho por reflectância total atenuada (HATRFTIR.Foram utilizadas 45 amostras de blendas biodiesel/diesel com concentrações de 8 a 30% de biodiesel e os espectros foramadquiridos em dois distintos espectrofotômetros e misturados aleatoriamente para a realização dos modelos, onde foram construídosmodelos para calibração utilizando 2/3 dos espectros das amostras obtendo assim os valores de RMSECV, e o restante dos espectrosforam empregados no conjunto de previsão, obtendo então os valores de RMSEP. Os dados espectrais foram autoescalados (AUTOou centrados na média (MEAN, com ou sem o emprego da correção multiplicativa de sinal (MSC. A utilização dos métodos deseleção das faixas espectrais aplicados aos espectros por ATR se mostrou viável para a quantificação do biodiesel nas blendas, sendoque a utilização da espectroscopia no infravermelho apresenta vantagens como à necessidade de pequena quantidade de amostra ebaixo tempo de análise, além de ser um procedimento não destrutivo e não gerador de resíduos, otimizando assim o processo emquestão.Abstract In the present work multivariate regressionmodels using interval partial least square (iPLS and backwardinterval partial least square (biPLS had been analyzed andcompared. iPLS and biPLS models had been developed todetermine the concentration of biodiesel in blends ofbiodiesel/diesel using infrared spectroscopy signals. 45samples with concentrations in range 8-30% of biodiesel, andtwo distinct spectrophotometers were

O dedo em baioneta e o uso de drogas ilícitas

OpenAIRE

Borini,Paulo

2001-01-01

A prevalência do "dedo em baioneta", sinal semiológico do distúrbio de déficit de atenção e hiperatividade (DHDA), foi determinada em pacientes do sexo masculino usuários de drogas ilícitas, internados em hospital psiquiátrico. Grupo controle, pareado quanto ao sexo, idade e classe social, foi selecionado da população geral. O sinal foi observado em 165/345 (47,8%) dos usuários de drogas e 13/50 (26%) dos controles, a diferença sendo estatisticamente significativa. Os portadores da anormalida...

Growth and characterization of ZnO{sub 1−x}S{sub x} highly mismatched alloys over the entire composition

Energy Technology Data Exchange (ETDEWEB)

Jaquez, M.; Ting, M. [Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (United States); Department of Mechanical Engineering, University of California, Berkeley, California 94720 (United States); Yu, K. M. [Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (United States); Department of Physics and Materials Science, City University of Hong Kong, Kowloon (Hong Kong); Hettick, M.; Javey, A. [Department of Electrical Engineering and Computer Sciences, University of California, Berkeley, California 94720 (United States); Joint Center for Artificial Photosynthesis, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (United States); Sánchez-Royo, J. F. [ICMUV, Instituto de Ciencia de Materiales, Universitat de València, P.O. Box 22085, 46071 Valencia (Spain); Wełna, M. [Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (United States); Department of Experimental Physics, Wrocław University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wrocław (Poland); Dubon, O. D. [Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (United States); Department of Materials Science and Engineering, University of California, Berkeley, California 94720 (United States); Walukiewicz, W., E-mail: w-walukiewicz@lbl.gov [Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (United States)

2015-12-07

Alloys from ZnO and ZnS have been synthesized by radio-frequency magnetron sputtering over the entire alloying range. The ZnO{sub 1−x}S{sub x} films are crystalline for all compositions. The optical absorption edge of these alloys decreases rapidly with small amount of added sulfur (x ∼ 0.02) and continues to red shift to a minimum of 2.6 eV at x = 0.45. At higher sulfur concentrations (x > 0.45), the absorption edge shows a continuous blue shift. The strong reduction in the band gap for O-rich alloys is the result of the upward shift of the valence-band edge with x as observed by x-ray photoelectron spectroscopy. As a result, the room temperature bandgap of ZnO{sub 1−x}S{sub x} alloys can be tuned from 3.7 eV to 2.6 eV. The observed large bowing in the composition dependence of the energy bandgap arises from the anticrossing interactions between (1) the valence-band of ZnO and the localized sulfur level at 0.30 eV above the ZnO valence-band maximum for O-rich alloys and (2) the conduction-band of ZnS and the localized oxygen level at 0.20 eV below the ZnS conduction band minimum for the S-rich alloys. The ability to tune the bandgap and knowledge of the location of the valence and conduction-band can be advantageous in applications, such as heterojunction solar cells, where band alignment is crucial.

Topotactic reduction of YBaCo2O5 and LaBaCo2O5: square-planar Co(I) in an extended oxide.

Science.gov (United States)

Seddon, James; Suard, Emmanuelle; Hayward, Michael A

2010-03-03

The low-temperature reduction of YBaCo(2)O(5) and LaBaCo(2)O(5) with NaH to form YBaCo(2)O(4.5) and YBaCo(2)O(4.25), respectively, demonstrates that the structures of anion-deficient materials formed by such topotactic reductions can be directed by the ordering and identity of the A-site cations. YBaCo(2)O(4.5) adopts a structure consisting of a corner-shared network of square-based pyramidal CoO(5) and distorted tetrahedral CoO(4) units. The structure of LaBaCoO(4.25) is more complex, consisting of an array of square-based pyramidal CoO(5), distorted tetrahedral CoO(4), and square planar CoO(4) units. Magnetic susceptibility and variable-temperature neutron diffraction data reveal that YBaCo(2)O(4.5) adopts a G-type antiferromagnetically ordered structure below T(N) approximately 280 K. LaBaCo(2)O(4.25) also adopts antiferromagnetic order (T(N) approximately 325 K) with ordered moments consistent with the presence of square-planar, low-spin, s = 0, Co(I) centers. A detailed analysis reveals that the different anion vacancy ordered structures adopted by the two REBaCo(2)O(5-x) phases are directed by the relative sizes and ordering of the La(3+) and Y(3+) cations. This suggests that ordered arrangements of A-cations can be used to direct the anion vacancy order in topotactically reduced phases, allowing the preparation of novel metal-oxygen networks containing unusual transition metal coordination environments.

Absolute rate constants for the reaction of CF3O2 and CF3O radicals with NO at 295 K

DEFF Research Database (Denmark)

Sehested, J.; Nielsen, O.J.

1993-01-01

Using a pulse radiolysis UV absorption technique and subsequent simulations of experimental NO2 and FNO absorption transients, rate constants for reaction between CF3O and CF3O2 radicals with NO were determined, CF3O2+NO-->CF3O+NO2 (3), CF3O+NO-->CF2O+FNO (5). k3 was derived to be (1.68+/-0.26)x10...

Efeito da rotação da cabeça na pressão intraocular em decúbito ventral: estudo randomizado

Directory of Open Access Journals (Sweden)

M. Nuri Deniz

2013-04-01

Full Text Available JUSTIFICATIVA E OBJETIVOS: A elevação da pressão intraocular (PIO, que diminui a pressão de perfusão do nervo óptico, é aumentada pelo posicionamento em decúbito ventral. O objetivo de nosso estudo foi comparar o efeito da rotação lateral da cabeça a 45º em decúbito ventral no aumento da PIO de olhos posicionados para cima e olhos posicionados para baixo em pacientes submetidos à nefrolitotomia percutânea (NLPC. MÉTODOS: Quarenta e cinco pacientes foram randomicamente alocados em dois grupos. A PIO dos pacientes foi registrada bilateralmente em posição supina antes do início da operação. Os pacientes foram posicionados em decúbito ventral. A cabeça foi posicionada sobre um apoio sem compressão externa direta em ambos os olhos. Os pacientes do Grupo I foram estritamente mantidos em pronação neutra, enquanto os pacientes do Grupo II foram posicionados em pronação com rotação da cabeça a 45º para o lado direito. No fim da operação, os pacientes foram reposicionados em decúbito dorsal e a PIO foi imediatamente medida. RESULTADOS: Não houve diferença entre os dados demográficos, na duração da cirurgia, perda de sangue e reposição de líquido dos pacientes. Os valores pós-cirúrgicos da PIO em decúbito ventral aumentaram significativamente em comparação com os valores pré-operatórios em ambos os grupos (p < 0,05. Após a cirurgia em decúbito ventral, os valores da PIO nos olhos posicionados para cima no Grupo II foram significativamente menores do que no Grupo I e nos olhos posicionados para baixo no Grupo II (p < 0,05. CONCLUSÃO: A posição em decúbito ventral aumenta a PIO. Nos pacientes posicionados em decúbito ventral com rotação lateral da cabeça a 45º, a PIO nos olhos posicionados para cima foi significativamente menor.

Effect of boron oxide addition on fibre drawing, mechanical properties and dissolution behaviour of phosphate-based glass fibres with fixed 40, 45 and 50 mol% P2O5.

Science.gov (United States)

Sharmin, Nusrat; Parsons, Andrew J; Rudd, Chris D; Ahmed, Ifty

2014-11-01

Previous studies investigating manufacture of phosphate-based glass fibres from glasses fixed with P2O5 content less than 50 mol% showed that continuous manufacture without breakage was very difficult. In this study, nine phosphate-based glass formulations from the system P2O5-CaO-Na2O-MgO-B2O3 were prepared with P2O5 contents fixed at 40, 45 and 50 mol%, where Na2O was replaced by 5 and 10 mol% B2O3 and MgO and CaO were fixed to 24 and 16 mol%, respectively. The effect of B2O3 addition on the fibre drawing, fibre mechanical properties and dissolution behaviour was investigated. It was found that addition of 5 and 10 mol% B2O3 enabled successful drawing of continuous fibres from glasses with phosphate (P2O5) contents fixed at 40, 45 and 50 mol%. The mechanical properties of the fibres were found to significantly increase with increasing B2O3 content. The highest tensile strength (1200 ± 130 MPa) was recorded for 45P2O5-16CaO-5Na2O-24MgO-10B2O3 glass fibres. The fibres were annealed, and a comparison of the mechanical properties and mode of degradation of annealed and non-annealed fibres were investigated. A decrease in tensile strength and an increase in tensile modulus were observed for the annealed fibres. An assessment of the change in mechanical properties of both the annealed and non-annealed fibres was performed in phosphate-buffered saline (PBS) at 37℃ for 28 and 60 days, respectively. Initial loss of mechanical properties due to annealing was found to be recovered with degradation. The B2O3-containing glass fibres were found to degrade at a much slower rate as compared to the non-B2O3-containing fibres. Both annealed and non-annealed fibres exhibited a peeling effect of the fibre's outer layer during degradation. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

Comparative genomic characterization of a Thailand-Myanmar isolate, MS6, of Vibrio cholerae O1 El Tor, which is phylogenetically related to a "US Gulf Coast" clone.

Science.gov (United States)

Okada, Kazuhisa; Na-Ubol, Mathukorn; Natakuathung, Wirongrong; Roobthaisong, Amonrattana; Maruyama, Fumito; Nakagawa, Ichiro; Chantaroj, Siriporn; Hamada, Shigeyuki

2014-01-01

The cholera outbreaks in Thailand during 2007-2010 were exclusively caused by the Vibrio cholerae O1 El Tor variant carrying the cholera toxin gene of the classical biotype. We previously isolated a V. cholerae O1 El Tor strain from a patient with diarrhea and designated it MS6. Multilocus sequence-typing analysis revealed that MS6 is most closely related to the U. S. Gulf Coast clone with the exception of two novel housekeeping genes. The nucleotide sequence of the genome of MS6 was determined and compared with those of 26 V. cholerae strains isolated from clinical and environmental sources worldwide. We show here that the MS6 isolate is distantly related to the ongoing seventh pandemic V. cholerae O1 El Tor strains. These strains differ with respect to polymorphisms in housekeeping genes, seventh pandemic group-specific markers, CTX phages, two genes encoding predicted transmembrane proteins, the presence of metY (MS6_A0927) or hchA/luxR in a highly conserved region of the V. cholerae O1 serogroup, and a superintegron (SI). We found that V. cholerae species carry either hchA/luxR or metY and that the V. cholerae O1 clade commonly possesses hchA/luxR, except for MS6 and U. S. Gulf Coast strains. These findings illuminate the evolutionary relationships among V. cholerae O1 strains. Moreover, the MS6 SI carries a quinolone-resistance gene cassette, which was closely related with those present in plasmid-borne integrons of other gram-negative bacteria. Phylogenetic analysis reveals that MS6 is most closely related to a U. S. Gulf Coast clone, indicating their divergence before that of the El Tor biotype strains from a common V. cholerae O1 ancestor. We propose that MS6 serves as an environmental aquatic reservoir of V. cholerae O1.

Crystallization behavior of Li2O-SiO2, Na2O-SiO2 and Na2O-CaO-SiO2 glasses; Li2O-SiO2, Na2O-SiO2, Na2O-CaO-SiO2 kei glass no kessho sekishutsu kyodo

Energy Technology Data Exchange (ETDEWEB)

Tsutsumi, K.; Otake, J.; Nagasaka, T.; Hino, M. [Tohoku University, Sendai (Japan)

1998-06-01

It has been known that crystallization of mold powder is effective on the disturbance of heat transfer between mold and solidified shell in production of middle carbon steel slabs in continuous casting process. But it has not yet been made clear which composition of mold powder is the most suitable for crystallization. The crystallization behavior of Li2O-SiO2, Na2O-SiO2 and Na2O-CaO-SiO2 glasses was observed by differential thermal analysis (DTA) and hot-thermocouple methods with DTA in the present work. As a result, addition of alkaline metal and alkaline earth metal oxides to SiO2 increased the critical cooling rate for glass formation in binary system of Li2O-SiO2 and Na2O-SiO2 and Li2O-SiO2 system crystallized easier than Na2O-SiO2 system. In ternary system of Na2O-CaO-SiO2, addition of Na2O hurried the critical cooling rate at CaO/SiO2=0.93 mass ratio, but the rate was almost constant in the composition range of more than 15 mass% Na2O. The slag of CaO/SiO2=0.93 made the rate faster than the slag of CaO/SiO2=0.47 at constant content of 10mass% Na2O. 17 refs., 10 figs., 3 tabs.

Heterogeneity in Induction Level, Infection Ability, and Morphology of Shiga Toxin-Encoding Phages (Stx Phages) from Dairy and Human Shiga Toxin-Producing Escherichia coli O26:H11 Isolates

Science.gov (United States)

Bonanno, Ludivine; Petit, Marie-Agnès; Loukiadis, Estelle; Michel, Valérie

2016-01-01

Shiga toxin (Stx)-producing Escherichia coli (STEC) bacteria are foodborne pathogens responsible for diarrhea and hemolytic-uremic syndrome (HUS). Shiga toxin, the main STEC virulence factor, is encoded by the stx gene located in the genome of a bacteriophage inserted into the bacterial chromosome. The O26:H11 serotype is considered to be the second-most-significant HUS-causing serotype worldwide after O157:H7. STEC O26:H11 bacteria and their stx-negative counterparts have been detected in dairy products. They may convert from the one form to the other by loss or acquisition of Stx phages, potentially confounding food microbiological diagnostic methods based on stx gene detection. Here we investigated the diversity and mobility of Stx phages from human and dairy STEC O26:H11 strains. Evaluation of their rate of in vitro induction, occurring either spontaneously or in the presence of mitomycin C, showed that the Stx2 phages were more inducible overall than Stx1 phages. However, no correlation was found between the Stx phage levels produced and the origin of the strains tested or the phage insertion sites. Morphological analysis by electron microscopy showed that Stx phages from STEC O26:H11 displayed various shapes that were unrelated to Stx1 or Stx2 types. Finally, the levels of sensitivity of stx-negative E. coli O26:H11 to six Stx phages differed among the 17 strains tested and our attempts to convert them into STEC were unsuccessful, indicating that their lysogenization was a rare event. PMID:26826235

Elevated O-GlcNAcylation stabilizes FOXM1 by its reduced degradation through GSK-3β inactivation in a human gastric carcinoma cell line, MKN45 cells.

Science.gov (United States)

Inoue, Yosuke; Moriwaki, Kazumasa; Ueda, Yasuhiro; Takeuchi, Toshihisa; Higuchi, Kazuhide; Asahi, Michio

2018-01-08

O-GlcNAcylation is a dynamic post-translational modification of cytonuclear proteins for intracellular signaling. Elevated O-GlcNAcylation is a general feature of cancer and contributes to cancer progression, and recent studies indicate the contribution to increasing incidence of various types of cancer in diabetic patients. However, the role of O-GlcNAcylation in tumor progression is not fully elucidated. Forkhead box M1 (FOXM1), a master mitotic transcription factor, has been implicated in all major hallmarks of cancer, and is wildly expressed in solid tumors. Given that FOXM1 expression was reported to be elevated in gastric cancer, we examined the effect of high glucose or an inhibitor of O-GlcNAc hydrolase, Thiamet G (TMG), on FOXM1 protein expression in a human gastric cancer cell line, MKN45 cells, and confirmed that FOXM1 protein level and the cell proliferation were upregulated. To investigate the molecular mechanisms by which FOXM1 protein expression is regulated by O-GlcNAcylation, the effect of high glucose and TMG on FOXM1 ubiquitination was examined in MKN45 cells. As a result, the ubiquitination and degradation of FOXM1 protein were both suppressed by high glucose and TMG treatment. However, the O-GlcNAcylation was not detected on FOXM1 but not on GSK-3β. High glucose and TMG treatment increased phospho-serine 9 GSK-3β, an inactive form, and the degradation of FOXM1 protein was suppressed by treatment of GSK-3β inhibitors in MKN45 cells. Taken together, we suggest that high glucose and elevated O-GlcNAcylation stabilize FOXM1 protein by its reduced degradation via GSK-3β inactivation in MKN45 cells, suggesting that the higher risk of gastric cancer in diabetic patients could be partially due to O-GlcNAcylation-mediated FOXM1 stabilization. Copyright © 2017 Elsevier Inc. All rights reserved.

Aleitamento materno e caracterização dos hábitos alimentares na primeira infância: experiência de São Tomé e Príncipe

Directory of Open Access Journals (Sweden)

Diana e Silva

2014-09-01

Full Text Available Objetivos: avaliação da amamentação, da diversificação e frequência alimentar, em crianças de São Tomé e Príncipe (STP. Métodos: trata-se de uma amostra constituída por 1285 crianças. O protocolo incluiu a prevalência do aleitamento materno exclusivo (AME e Total (AMT, início da diversificação alimentar (DA e um questionário de frequência alimentar. O tratamento estatístico foi efetuado no SPSS®. Os resultados foram apresentados de acordo com o total da amostra. Resultados: 45,5% são do sexo feminino e a média de idades 26±18 meses. 46,6% fez AME até aos 6 meses (média 5±2. A média de AMT foi 12±7 meses e o início da DA aos 6±3 meses (mediana=6, sendo as farinhas e a canja de peixe os primeiros alimentos oferecidos. 42% das crianças são incluídas na dieta familiar aos 7±3 meses (mediana=6. Elevada porcentagem de crianças nunca ingere: leite de vaca (74% ou iogurte (40%, contrariamente ao elevado consumo de óleo alimentar (34% e açúcar (33%. A idade média de iniciação da cerveja é aos 18±12 meses e vinho de palma 13±10 meses. Conclusões: observa-se uma elevada prevalência de AME e uma precoce introdução da DA. Embora existam recursos alimentares disponíveis, não há informação/ formação adequada para elaborar um plano alimentar saudável durante a infância.

Phase relations in the SiC-Al2O3-Pr2O3 system

International Nuclear Information System (INIS)

Pan, W.; Wu, L.; Jiang, Y.; Huang, Z.

2016-01-01

Phase relations in the Si-Al-Pr-O-C system, including the SiC-Al 2 O 3 -Pr 2 O 3 , the Al 2 O 3 -Pr 2 O 3 -SiO 2 and the SiC-Al 2 O 3 -Pr 2 O 3 -SiO 2 subsystems, were determined by means of XRD phase analysis of solid-state-reacted samples fabricated by using SiC, Al 2 O 3 , Pr 2 O 3 and SiO 2 powders as the starting materials. Subsolidus phase diagrams of the systems were presented. Two Pr-aluminates, namely PrAlO 3 (PrAP) and PrAl 11 O 18 (β(Pr) β-Al 2 O 3 type) were formed in the SiC-Al 2 O 3 -Pr 2 O 3 system. SiC was compatible with both of them. Pr-silicates of Pr 2 SiO 5 , Pr 2 Si 2 O 7 and Pr 9.33 Si 6 O 26 (H(Pr) apatite type) were formed owing to presence of SiO 2 impurity in the SiC powder. The presence of the SiO 2 extended the ternary system of SiC-Al 2 O 3 -Pr 2 O 3 into a quaternary system of SiC-Al 2 O 3 -SiO 2 -Pr 2 O 3 (Si-Al-Pr-O-C). SiC was compatible with Al 2 O 3 , Pr 2 O 3 and the Pr-silicates. The effect of SiO 2 on the phase relations and liquid phase sintering of SiC ceramics was discussed.

Revisão de 26 casos clínicos de duodeno-jejunite proximal em eqüinos (1996-2000

Directory of Open Access Journals (Sweden)

Fernandes Wilson Roberto

2003-01-01

Full Text Available Os dados de 26 eqüinos com duodeno-jejunite proximal (DJP, examinados no HOVET-FMVZ-USP entre dezembro de 1996 e novembro de 2000, foram revisados. Durante esse período, foram atendidos 1555 animais, dos quais 205 apresentavam distúrbios gastrintestinais (13,2%. Os casos de DJP representaram 1,7% do total de eqüinos atendidos. A idade, os achados clínico-laboratoriais e a evolução clínica foram comparados entre eqüinos sobreviventes (grupo 1 e eqüinos não sobreviventes (grupo 2. Vinte eqüinos (76,9% sobreviveram. Todos os animais foram submetidos exclusivamente a tratamento médico. A análise dos resultados foi feita através de comparação entre médias pelo teste t de Student com significância de 5%. Houve diferença significativa entre os dois grupos em relação aos seguintes parâmetros analisados: contagem total de leucócitos no sangue, creatinina sérica e freqüência cardíaca. A principal complicação nos animais recuperados foi laminite (30,8%.

Avaliação da satisfação dos pacientes submetidos à intervenção fisioterapêutica no município de Campo Maior, PI

Directory of Open Access Journals (Sweden)

Juscelino Rodrigues Gonçalves

Full Text Available INTRODUÇÃO: Buscar satisfazer as necessidades dos usuários de serviços de saúde consiste em uma forma de medir a qualidade da atenção oferecida pelos profissionais desse setor, ainda que a qualidade da assistência continue sendo uma grande preocupação na saúde. OBJETIVO: Avaliar a satisfação dos pacientes em tratamento fisioterapêutico em quatro clínicas do município de Campo Maior, Piauí. MATERIAIS E MÉTODOS: Foi realizado um estudo descritivo e transversal em uma amostra de 80 pacientes. A pesquisa consistiu na aplicação de um questionário contendo 20 perguntas objetivas para avaliação da satisfação dos serviços oferecidos pelas clínicas selecionadas. RESULTADOS: Dos 80 entrevistados, 55% eram do gênero feminino, 26,25% pertenciam à faixa etária de 40 a 51 anos (26,25%, 46,75% fizeram o ensino fundamental, 66,75% possuíam renda mensal de 1 a 3 salários minimos e 66,25% não disponibilizavam de planos de saúde. A maioria dos indivíduos foi encaminhada por médicos e considerou a clínica de fácil acesso; 55% utilizava transporte particular ou alugado para locomoção; e a indicação de terceiros foi o principal motivo de escolha do serviço (65%. Os pacientes, em grande parte, mostraram-se satisfeitos com o tempo de espera para consulta, atendimento e uso dos aparelhos e confirmaram haver ótima relação com o fisioterapeuta. Os entrevistados ainda relataram ótima limpeza, conforto e boas instalações e consideraram suas necessidades de tratamento atendidas. CONCLUSÃO: No geral, observou-se grande satisfação dos usuários dos serviços de fisioterapia do município de Campo Maior. Entretanto, outros estudos se fazem necessários, correlacionando o grau de satisfação aos dados socioeconômicos.

Facilitação Neuromuscular Proprioceptiva e o Alongamento Muscular como Modalidades Terapêuticas na Disfunção Temporomandibular

Directory of Open Access Journals (Sweden)

Luana Torres Monteiro Melo

2014-09-01

Full Text Available Introdução: O fisioterapeuta, integrante da equipe multidisciplinar, utiliza ampla variedade de aparelhos e recursos manuais no tratamento da disfunção temporomandibular (DTM, buscando recuperar a função do sistema estomatognático. A facilitação neuromuscular proprioceptiva (FNP promove o movimento funcional por meio da facilitação, inibição e relaxamento da musculatura. O alongamento muscular reduz as tensões musculares e libera os movimentos bloqueados. Objetivo: Comparar os benefícios das duas técnicas na melhoria funcional de pacientes portadores de DTM.  Métodos: Realizou-se estudo intervencionista, comparativo e cego, com 12 pacientes atendidas no Núcleo de Atenção Médica Integrada (NAMI, Fortaleza-Ceará, do sexo feminino, adultas e com diagnóstico de DTM, divididas aleatoriamente em dois grupos. O grupo A foi tratado com FNP (técnica de manter-relaxar e estabilização rítmica e o grupo B com alongamento muscular passivo. O tratamento foi realizado 2 vezes na semana, durante 5 semanas, totalizando 10 atendimentos com duração de 45 minutos. Resultados: Foi verificado que as pacientes de ambos os grupos tiveram melhora do quadro clínico, com redução significativa da dor em ambos os grupos (p < 0,05 e diminuição da dor à palpação muscular no grupo B (p < 0,05. Ao final do tratamento, não houve diferença na sintomatologia, amplitude de movimento e dor à palpação muscular entre os grupos. Conclusão: Os resultados mostraram que tanto a FNP como o alongamento muscular promoveram melhora do quadro álgico das pacientes, concluindo-se que ambas as técnicas podem ser acrescentadas aos tratamentos existentes para essa disfunção.

Vitreous System Ag2O –ZnO–B2O3 Action Against Gram Negative Bacteria

Directory of Open Access Journals (Sweden)

Razvan Stefan

2010-05-01

Full Text Available In this work the ability of the system xAg2O  (100-x [45ZnO55B2O3] oxide glasses to inhibit the growth of bacteria Eschierchia coli, Pseudomonas and Salmonella was investigated. Using the diffusimetric method there were measured the diameters of inhibition, in order to classify the sensitivity of gram negative bacteria to oxide compounds containing silver, for their use as filters. Vitreous samples were processed as powders with grain between 45 and 75 m and less than 45 m for a large of contact area with the microorgansms and to make possible the study of biological effect of grain addiction. Action of the investigated oxide system against the gram negative bacteria is strictly related to the presence of silver oxide in glass composition.