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Sample records for seed vigour imaging

  1. Seed vigour and crop establishment: extending performance beyond adaptation.

    Science.gov (United States)

    Finch-Savage, W E; Bassel, G W

    2016-02-01

    Seeds are central to crop production, human nutrition, and food security. A key component of the performance of crop seeds is the complex trait of seed vigour. Crop yield and resource use efficiency depend on successful plant establishment in the field, and it is the vigour of seeds that defines their ability to germinate and establish seedlings rapidly, uniformly, and robustly across diverse environmental conditions. Improving vigour to enhance the critical and yield-defining stage of crop establishment remains a primary objective of the agricultural industry and the seed/breeding companies that support it. Our knowledge of the regulation of seed germination has developed greatly in recent times, yet understanding of the basis of variation in vigour and therefore seed performance during the establishment of crops remains limited. Here we consider seed vigour at an ecophysiological, molecular, and biomechanical level. We discuss how some seed characteristics that serve as adaptive responses to the natural environment are not suitable for agriculture. Past domestication has provided incremental improvements, but further actively directed change is required to produce seeds with the characteristics required both now and in the future. We discuss ways in which basic plant science could be applied to enhance seed performance in crop production. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  2. Dependence of the legume seeds vigour on their maturity and method of harvest

    Directory of Open Access Journals (Sweden)

    Stanisław Grzesiuk

    2014-01-01

    Full Text Available Several methods were used to study 'the vigour and viability of legume seeds (Pisum sativum L. cv. Hamil, Piston arvense L. cv. Mazurska and Lupinus luteus L. cv. Tomik harvested at three main stages of seed repening (green, wax and full. The seeds were tested immediately after harvest (series A and after two weeks of storage in pods (series B. It was found that: 1 the vigour of ripening legume seeds increases with maturation; 2 post-harvest storage in pods increases the degree of ripeness and. consequently. vigour; 3 seeds attain full vigour later than full viability; 4 seed leachate conductivity method gives erroneous results in assessing the vigour of immature seeds: 5 full vigour of maturing seeds of various degrees of ripeness can be determined by simultaneous application of both biological (eg. seedling growth analysis, VI and biochemical (e.g. total dehydrogenase activity methods.

  3. A systems genetics study of seed quality and seedling vigour in Brassica rapa

    NARCIS (Netherlands)

    Basnet, R.K.

    2015-01-01

    Summary

    Seed is the basic and most critical input for seed propagated agricultural crops: seed quality and seedling vigour determine plant establishment, growth and development in both natural and agricultural ecosystems. Seed quality and seedling vigour are mainly

  4. Genetic dissection of seed vigour traits in maize (Zea mays L.) under ...

    Indian Academy of Sciences (India)

    YONG SHI

    ity, reflects potential seed germination, seedling growth, seed ... Keywords. recombinant inbred line population; seed vigour; quantitative trait locus analysis; cold tolerance. ..... nent youth fund of Henan province, Corn Industry Technology Sys-.

  5. Physical methods for seed vigourization: advantages and challenges in seed technology

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    Susana eAraújo

    2016-05-01

    Full Text Available In the context of seed technology, the use of physical methods for increasing plant production offers advantages over conventional treatments based on chemical substances. The effects of physical vigourization treatments in seeds can be now addressed at multiple levels, ranging from morpho-structural aspects to changes in gene expression and protein or metabolite accumulation. Among the physical methods available, magneto-priming and irradiation with microwaves or ionizing radiations are the most promissory pre-sowing seed treatments. Magneto-priming is based on the application of magnetic fields and described as an eco-friendly, cheap, non-invasive technique with proved beneficial effects on seed germination, vigour and crop yield. Ionizing radiations, as gamma-rays and X-rays, have been widely regarded as a powerful tool in agricultural sciences and food technology. Gamma-rays delivered at low dose have showed to enhance germination percentage and seedling establishment, acting as an actual ‘priming’ treatment. Different biological effects have been observed in seeds subjected to microwaves and X-rays but knowledge about their impact as seed vigourization agent or stimulatory effects on germination need to be further extended. Ultraviolet (UV radiations, namely UV-A and UV-C have shown to stimulate positive impacts on seed health, germination and seedling vigour. For all mentioned physical treatments, extensive fundamental and applied research is still needed to define the optimal dose, exposition time, genotype- and environment-dependent irradiation conditions. Electron paramagnetic resonance (EPR has an enormous potential in seed technology not fully explored to monitor seed vigourization treatments and/or identifying the best suitable irradiation dose or time-point to stop the treatment. The present manuscript describes the use of physical methods for seed vigourization, while providing a critical discussion on the constraints and

  6. Effect of the vigour and of the fungicide treatment in the germination and sanity tests of soybean seeds

    OpenAIRE

    Gomes, Delineide Pereira; Barrozo, Leandra Matos; Souza, Apolyana Lorraine; Sader, Rubens [UNESP; Silva, Gilvania Campos [UNESP

    2009-01-01

    Soybeans seeds with different levels of vigour, treated and or no with fungicide were submitted to the test of germination in the sand and roll substrates of paper and the test of sanity. In test of germination, there were only significant differences between treatments that used soybeans with high vigour and with low vigour, regardless of the presence or absence of fungicide treatment, in the two substrates. The soybeans with low vigour no treated provided the largest percentages of seed con...

  7. Seed vigour tests for predicting field emergence of maize under severe conditions

    OpenAIRE

    García de Yzaguirre, Álvaro; Lasa Dolhagaray, José Manuel

    1989-01-01

    [EN] With 40 to 50 different seed vigour tests available, appropiate procedures for choosing the best single test or combination the best predictors of seedling emergence of maize (Zea Mays L.) under severe conditions. Thirteen vigour tests and various field emergence trials were performed on six inbred lines and two commercial hybrids. The best single predictors of field emergence were identified by calculating simple correlation coefficients. The calculation of the geometric mean of the res...

  8. Use of tetrazolium (TTC, Germ's and greenhouse plant emergences methods for testing seed vigour of selected ornamental plant species

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    Roman Hołubowicz

    2013-12-01

    Full Text Available In the years 1996-1997 the experiments were carried out on methods to investigate seed vigour of tassel flower (Amaranthus caudatus L., sand pink (Dianthus chinensis L., babies' breath (Gypsophila elegans M.B., sweet pea (Lathyrus odorathus L., African marigold (Tagetes erecta L. and zinnia (Zinnia elegans Jasq.. The main goals of this research were to specify conditions for accelerated ageing (AA of the seeds of a few selected ornamental plant species and to choose the most appropriate methods for their seed vigour evaluation in the laboratory and greenhouse conditions. All used in the experiments seeds came from the commercial seed lots from Polish seed company. Evaluation was carried out on the seed samples with high and low vigour. The latter ones were received through subjecting the seed samples to AA, i.e. by placing them in 100% relative humidity (RH at 44°C, except African marigold-at 42°C, in the darkness and keeping them for 144, 88, 100, 48, 72 and 72 hours, respectively. The tested seed vigour estimated methods included the Germ's method, the 2,3,5-triphenyl tetrazoilum chloride (TTC method and the test of plant emergences in the greenhouse. The high vigour seeds samples were used as a check. The Germ's method was found to be useful to evaluate sand pink, babies' breath and African marigold seed vigour, whereas the TTC method was found to be suitable for vigour evaluation of sand pink, babies' breath and zinnia. At present stage of our knowledge about seed vigour, the plant emergences in the greenhouse method was found to be the best for evaluation of seed vigour of tassel flower, sand pink, babies' breath, sweet pea and zinnia. It is reasonable to combine a few methods of seed vigour evaluation for ornamental plant species.

  9. Studies on the Vigour of Soybean Seeds : II. Varietal Differences in Seed Coat Quality and Swelling Components of Seed during Moisture Imbibition

    OpenAIRE

    Mugnisjah, Wahju Qamara; Shimano, Itaru; Matsumoto, Shigeo; 島野, 至; 松本, 重男

    1987-01-01

    Laboratory experiment was conducted to elucidate the physiological factor determining the association of seed size and seed coat quality with varietal differences in seed vigour, and to pursue an alternative on the mechanism of varietal differences in seed resistance to field weathering. Results of this study revealed that seed polymer change (seed volume change minus seed weight change during moisture imbibition) was the physiological factor determining the association of seed size and seed ...

  10. Effect of gamma radiation on seed germination and seedling vigour in cowpea [Vigna unguiculata (L.) Walp.

    International Nuclear Information System (INIS)

    Thimmaiah, S.K.; Mahadevu, P; Srinivasappa, K.N.; Shankara, A.N.

    1998-01-01

    Cowpea [Vigna unguiculata (L.) Walp.) is regarded as hardy and one of the important tropical legumes. The plants respond differently to mutagenic treatments. Ionizing radiations affect a wide range of physiological and biochemical activities of plants. The purpose of this paper is to report the effect of gamma radiation on seed germination and seedling vigour of two important cowpea varieties viz., KBC-1 and TVX-994-02E in M 1 generation under laboratory conditions. (author)

  11. Studies on germination and vigour of cabbage seeds = [Aspecten van de kieming van zaden van witte kool en savoiekool

    NARCIS (Netherlands)

    Liou, T.D.

    1987-01-01

    The effects of commercial storage of cabbage seeds on the germination of seeds and the emergence and growth of seedlings have been studied. Progressive ageing of seeds caused loss of seed vigour which resulted in poor emergence and growth of seedlings and the formation of abnormal

  12. ACCELERATED AGING TEST IN DETERMINING THE VIGOUR OF SUNFLOWER SEEDS WITH AND WITHOUT PERICARP

    Directory of Open Access Journals (Sweden)

    K. R. Ducatti

    2014-09-01

    Full Text Available The standard germination test alone is insufficient to attest the quality of seeds, making necessary correlation’s with vigour tests, to determine more accurately the physiological potential of a seeds lot. The accelerated aging test is an option for determine the vigour and consists in submits seeds to high temperatures and humidity, for different periods of time, has not yet standardized. The objective of this work was to analyze the efficiency of the accelerated aging test in the assessment of the effect of a lot of sunflower seed, by three periods of aging (48, 56 and 72 hours in 42 °C temperature, in seeds with and without pericarp (manually removed and relate the results with electrical conductivity test and germination first count. The experimental design was a completely randomized design and the comparison of averages made using Tukey's test at 5% probability. The results showed that standard germination, electrical conductivity and germination first count, the seeds without pericarp showed better performance. In relation to the accelerated aging, only in the period of 72 hours of aging there was no significant difference between the treatments. In this way, the appropriate period to identify differences in force between the treatments was 72 hours, which showed a positive correlation with the germination first count and electrical conductivity.

  13. Quantitative Trait Locus Analysis of seed germination and seedling vigour in Brassica rapa reveals QTL hotspots and epistatic interactions

    NARCIS (Netherlands)

    Basnet, R.K.; Duwal, A.; Tiwari, D.N.; Xiao, D.; Monakhos, S.; Bucher, J.; Visser, R.G.F.; Groot, S.P.C.; Bonnema, A.B.; Maliepaard, C.A.

    2015-01-01

    The genetic basis of seed germination and seedling vigour is largely unknown in Brassica species. We performed a study to evaluate the genetic basis of these important traits in a B. rapa doubled haploid population from a cross of a yellow-seeded oil-type yellow sarson and a black-seeded

  14. Genetic dissection of seed vigour traits in maize (Zea mays L.) under ...

    Indian Academy of Sciences (India)

    subjected to cold stress and frequently exhibit poor early- season vigour that ... Yong Shi, Guohui Li and Zhiqiang Tian contributed equally to this work. Yu82 × Shen137 is .... Other vigour-related traits within the three parental lines exhibited no ...

  15. Genetic dissection of seed vigour traits in maize (Zea mays L.) under ...

    Indian Academy of Sciences (India)

    YONG SHI

    measurements, the seeds of each genotype were divided into three portions, each consisting of 50 seeds, ... ance (ANOVA) using the general linear model procedure of the statistical software SPSS 12.0. Descriptive ... the phenotypic variation, with five QTL accounting for more than 10% of the total phenotypic variation.

  16. Germination and vigour of primed asparagus seeds Germinação e vigor de sementes de aspargo osmoticamente condicionadas

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    Mário Lúcio de Carvalho Bittencourt

    2005-08-01

    Full Text Available The osmotic conditioning or priming is considered a promising technique to speed up germination and to improve seed performance. Four seed lots of asparagus, Mary Washington cultivar, were primed at 25°C for seven or 14 days using PEG 6000 at -1.0 or -1.2 MPa, or sea water at -3.3 MPa; or for three days in distilled water. The physiological quality of the seeds was evaluated by standard germination, first count germination, speed of seedling emergence, and germination percentage and seedling fresh and dry weights after controlled deterioration test. Primed seeds presented higher germination speed, independently of their initial physiological quality. Beneficial effects of priming on germination and vigour were more expressive in the seed lot of low physiological quality. Priming in PEG 6000 at -1.0 MPa for 14 days was the most beneficial treatment to improve asparagus seeds performance.O condicionamento osmótico ou priming tem sido considerado uma técnica promissora para acelerar a germinação e melhorar o desempenho das sementes. Sementes de quatro lotes de aspargo, cv. Mary Washington, foram condicionadas em PEG 6000 a -1,0 e -1,2 MPa, por 7 e 14 dias, em água do mar a -3,3 MPa, por 7 e 14 dias e em água destilada por 3 dias, em BOD a 25°C. Sementes não condicionadas foram usadas como testemunha. A qualidade fisiológica das sementes foi avaliada pela germinação, primeira contagem de germinação, velocidade de emergência e pela porcentagem de geminação e pesos da matéria fresca e seca das plântulas obtidas no teste de deterioração controlada (45°C/24 horas. O experimento foi instalado no delineamento inteiramente casualizado com quatro repetições e oito tratamentos, sendo as médias comparadas pelo teste de Duncan a 5%. Sementes de aspargo condicionadas osmoticamente apresentaram maior velocidade de germinação, independentemente de sua qualidade fisiológica inicial. Efeitos benéficos do condicionamento osmótico, na

  17. Survival and vigour of ultra-dry seeds after ten years of hermetic storage

    NARCIS (Netherlands)

    Hong, T.D.; Ellis, R.H.; Astley, D.; Pinnegar, A.E.; Groot, S.P.C.; Kraak, H.L.

    2005-01-01

    Seeds of carrot, groundnut, lettuce, oilseed rape and onion were stored hermetically in laminated aluminium foil packets in four environments (dry or ultra-dry moisture contents combined factorially with temperatures of 20 degrees C or -20 degrees C), replicated at several sites. After ten years'

  18. Conservação e vigor de sementes de pau-de-balsa (Ochroma pyramidale Conservation and vigour of balsawood seeds (Ochroma pyramidale

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    Antonio Moçambite Pinto

    2004-01-01

    Full Text Available Ochroma pyramidale, Bombacaceae, conhecida popularmente como pau-de-balsa, é utilizada para construção de jangadas, balsas, salva-vidas, bóias, brinquedos e na fabricação de papel e celulose. O objetivo deste estudo foi definir um método de acondicionamento de sementes de O. pyramidale, visando a conservação da viabilidade e vigor destas para sua utilização e comercialização em épocas de baixa produção. Sementes de O. pyramidale foram embaladas em sacos de papel tipo kraft e sacos de plástico (0,10 mm e armazenadas em ambiente de laboratório (22ºC e 65% U.R., câmara úmida (5ºC e 86% U.R. e câmara seca (15ºC e 40% U.R.. A percentagem de germinação, teor de água e vigor das sementes foram avaliados no início e após períodos de armazenamento. Todos os tratamentos testados foram favoráveis para manutenção do vigor das sementes por 120 dias de armazenamento. As melhores condições de armazenamento para manter a viabilidade por até 400 dias foram: sacos de papel (76,5% de germinação e sacos plásticos (65,5% de germinação em câmara seca, e sacos plásticos em condições de laboratório (63,5% de germinação.Balsawood (Ochroma pyramidale, Bombacaceae is used for construction of rafts, floats, life-savers, buoys, toys and for paper and cellulose production. The objective of this study was to determine a seed storage method for O. pyramidale to conserve seed viability and vigour for use and commercialization during seasonal shortages. Seeds were put in paper (Kraft and plastic bags (0.10 mm, and stored in three environmental conditions: laboratory (22ºC and 65% relative humidity, humid chamber (5ºC and 86% RH and dry chamber (15ºC and 40% RH. Germination percentage, moisture content and vigour of seeds were evaluated at the beginning of the experiment and after the storage periods. All treatments maintained seed vigour for 120 days of storage. The best storage conditions to maintain seed viability for a 400

  19. The Variability of Seed Viability and Seed Vigour of Mindi (Melia azedarachlinn. from Several Populations in The Community Forest of West Java

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    Yulianti Bramasto

    2016-12-01

    Full Text Available The procurement of good quality seeds for the development of mindi (Melia azedarach L. in the community forest is absolutely necessary. The purpose of this research is to investigate the diversity of the viability and seedling vigor of mindi from different populations with various treatments of dormancy breaking. The sample materials were mindi seeds that were collected from 6 locations (6 seed lots or seed sources which were located in the community forest of West Java. There are 10 treatments of dormancy breaking that were applied in this study. The experiment design was Randomized Complete Design (RAL. The breaking dormancy  was focused to the delignification of hard seed coat.  Results showed that the most effective dormancy breaking for mindi seed was soaking in consentrated Sulfuric Acid (H2SO4 as long as 30 to 45 minutes. The first count for germination of mindi was on the 16 day and the final count was on the 31 day.The average value of germination percentage (DB for all seed sources was 40 %, while the highest which is 60% was mindi from Sumedang seed source. The highest value of germination rate that is 6,543%/etmal was seed  from Sumedangand seed from Gambung was the lowest (1,400 %/etmal. Keywords : community forest, Melia azedarach L., variation, viability, vigor.

  20. Teste de lixiviação de potássio para avaliação do vigor de sementes de soja [Glycine max (L.Merril] Potassium leaching test for the evaluation of soybean seed vigour

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    D.C.F.S. Dias

    1995-12-01

    Full Text Available O presente trabalho teve como objetivo principal investigar a possibilidade de se obter indicações rápidas sobre a qualidade fisiológica de sementes de soja através do teste de lixiviação de potássio, cuja eficiência foi avaliada comparativamente as informações fornecidas por outros métodos considerados adequados para a determinação do vigor. Para tanto, utilizou-se quatro lotes de sementes de soja dos cultivares IAC-8 e IAC-15 que foram submetidos aos testes de germinação, primeira contagem de germinação, envelhecimento artificial, condutividade elétrica e determinação do grau de umidade. Além destes foram conduzidos estudos de lixiviação de potássio utilizando-se amostras de sementes não danificadas e de sementes fisicamente puras. A quantidade de potássio lixiviado foi avaliada em fotômetro de chama após 60, 90, 120 e 150 minutos de embebição a 30ºC. As avaliações feitas aos 60, 90 e 120 minutos mostraram-se adequadas para a identificação de lotes com diferentes níveis de vigor, constituindo-se em um método simples e rápido para a avaliação da qualidade fisiológica das sementes.This work was performed to investigate the possibilities of providing rapid indications on the physiological quality of seeds through the potassium leaching test. Four lots of soybean (Glycine max (L. Merrill seeds, cultivars IAC-8 and IAC-15 were submitted to germination, first count, accelerated aging, electrical conductivity and seed moisture tests. In addition, studies on potassium leaching were conducted with samples of selected non damaged seeds and with pure seeds. The amount of leached potassium was evaluated in a flame photometer after a 60, 90, 120 and 150 minute imbibition at 30°C. The evaluations after 60, 90 and 120 minutes were suitable for the identification of lots with different levels of vigour, thus proving to be a simple and rapid method for seed vigour evaluation.

  1. Efeito do tratamento com fontes de zinco e boro na germinação e vigor de sementes de milho Effects of maize seed treated with zinc and boron sources on germination and vigour

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    N.D. Ribeiro

    1994-12-01

    Full Text Available Foi conduzido um experimento no Departamento de Fitotecnia da Universidade Federal de Santa Maria, RS, no período de março a novembro de 1992, com o objetivo de verificar o efeito da aplicação de fontes de zinco e boro em sementes de milho tratadas ou não com defensivos agrícolas. Adotou-se o esquema fatorial 3x2x6, em delineamento inteiramente casualizado, com quatro repetições. Os tratamentos constaram de avaliações em três épocas (logo após o tratamento, quatro e oito meses depois, utilização de sementes de milho com e sem tratamento fítossanitário, combinados com seis fontes de zinco e boro (testemunha, Zn-Biocrop, B-Biocrop, E-Orgânico, Zn-Biocrop + B-Biocrop e Zn-Biocrop + B-Orgânico, nas doses únicas de 2,50g Zn e de 0,15g B/kg de sementes. Os resultados obtidos mostram que a aplicação da fonte Zn-Biocrop não prejudica a germinação e o vigor, pelo período de oito meses de armazenamento. O tratamento de sementes com boro (B-Biocrop e B-Orgânico diminui a germinação e o vigor.The experiment was carried during the period of march 1992 to november 1992, to study the effects of maize seed treatment with zinc, boron and pesticides, on the germination and vigour during storage. The experimental design was a complete randomized 3x2x6 fatorial with four replicátions. The treatments were three storage periods (zero, four and eight month, pesticides treatments with or without, and six sources of zinc and boron (control, Zn-Biocrop, B-Biocrop, Organic-B, Zn-Biocrop -I- B-Biocrop and Zn-Biocrop + Organic-B, in the dose 2.50g of the Zn and 0.l5g of the B/kg of seeds. The results show that Zn-Biocrop maintain high germination and vigour for eight month of storage. The boron treatment (B-Biocrop and Organic-B showed a low germination and vigour.

  2. Testes de envelhecimento acelerado e deterioração controlada na avaliação do vigor de aquênios de girassol Accelerated aging and controlled deterioration seeds vigour tests for sunflower

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    Madelon Rodrigues Sá Braz

    2008-10-01

    Full Text Available Os testes de vigor têm sido desenvolvidos para diferenciar os lotes de sementes devido às limitações impostas pelo teste de germinação. O objetivo do trabalho foi analisar a eficiência dos testes de envelhecimento acelerado e deterioração controlada na avaliação do vigor de lotes de aquênios de girassol. Para tanto, cinco lotes de aquênios (sementes com o pericarpo de girassol da cultivar "Catissol 01" foram submetidos à determinação do grau de umidade e à avaliação da qualidade fisiológica, empregando os testes de germinação e vigor (primeira contagem, teste de frio, condutividade elétrica, emergência de plântulas, índice de velocidade de emergência, envelhecimento acelerado pelos sistemas tradicional e com solução saturada de NaCl a 42°C por 48, 72, 96 horas e deterioração controlada com teor inicial de água de 15, 20, 25% por 48, 72, 96 horas a 42°C. Os resultados permitiram concluir que o teste de envelhecimento acelerado com solução saturada de NaCl por 96 horas foi eficiente para classificar os lotes de aquênios de girassol. As combinações de 20% de água nos aquênios por 72 horas de exposição e de 25% de água nos aquênios por 48 e 72 horas de exposição permitem classificar os lotes de aquênios de girassol.The vigour tests were developed to detect differences of seeds lots owing to limitations the germination test. This study was conducted to compare different procedures of the accelerated aging and controlled deterioration tests to evaluate sunflowers seed vigour. Five lots of sunflower achenes (seeds with pericarp cv. Catissol 01 were submitted to the water content test and to the evaluation of physiological quality using by germination and vigour test (first count, cold test, electrical conductivity, seedling emergence and speed of emergence index, accelerated aging with tradicional system and satured solution of NaCl, at 42°C for 48, 72, 96 hours and controlled deterioration with 15, 20

  3. Sorghum [Sorghum bicolor (L.) Moench] Seed Quality as Affected by ...

    African Journals Online (AJOL)

    ... bicolor (L.) Moench seeds subjected to different field cultural management practices. ... Germination and vigour tests indicated that seed selection time did not ... In relation to this, variety E1291 showed better seed vigour, viability and yield ...

  4. Hybrid vigour and gene action for two quantitative traits of castor ...

    African Journals Online (AJOL)

    Five homozygous lines of castor plant, namely RS1-Om, RN1-Om, RTl-2m, RSl- Owm, and RNl- Omb, were crossed to raise F1, F2, BC1, and BC2 generations. The hybrids were tested for hybrid vigour for two metric traits, viz; number of pods per plant and seed yield per plant. Highly significant hybrid vigour was detected ...

  5. Image analysis in the evaluation of the physiological potential of maize seeds1

    Directory of Open Access Journals (Sweden)

    Crislaine Aparecida Gomes Pinto

    Full Text Available The Seed Analysis System (SAS is used in the image analysis of seeds and seedlings, and has the potential for use in the control of seed quality. The aim of this research was to adapt the methodology of image analysis of maize seedlings by SAS, and to verify the potential use of this equipment in the evaluation of the physiological potential of maize seeds. Nine batches of two maize hybrids were characterised by means of the following tests and determinations: germination, first count, accelerated ageing, cold test, seedling emergence at 25 and 30ºC, and speed of emergence index. The image analysis experiment was carried out in a factorial scheme of 9 batches x 4 methods of analysis of the seedling images (with and without the use of NWF as substrate, and with and without manual correction of the images. Images of the seedlings were evaluated using the average lengths of the coleoptile, roots and seedlings; and by the automatic and manual indices of vigour, uniformity and growth produced by the SAS. Use of blue NWF afffects the initial development of maize seedlings. The physiological potential of maize seeds can be evaluated in seedlings which are seeded on white paper towels at a temperature of 25 °C and evaluated on the third day. Image analysis should be carried out with the SAS software using automatic calibration and with no correction of the seedling images. Use of SAS equipment for the analysis of seedling images is a potential tool in evaluating the physiological quality of maize seeds.

  6. on seed germination and growth of Garcinia kola

    African Journals Online (AJOL)

    SARAH

    2016-07-31

    Jul 31, 2016 ... Parameters related to seed germination and seedlings vigour was evaluated. Results indicated that substrate do not affect seed germination and plant vigour. However ..... Annual plant reviews California, USA, pp. 50-. 6.7.

  7. The effect of high dilutions of Pulsatilla nigricans on the vigour of soybean seeds subjected to accelerated aging=O efeito de altas diluições de Pulsatilla nigricans no vigor de sementes de soja submetidas ao envelhecimento acelerado

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    Bruno Reis

    2012-04-01

    Full Text Available The aim of this study was to evaluate the effect of high dilutions of Pulsatilla nigricans in dinamisations 6, 12, 18, 24 and 30 CH on the vigour of soybean seeds subjected to accelerated aging. The experiment was conducted according to a randomised design with 6 treatments and 10 replicates. The treatments consisted of dinamisations 6, 12, 18, 24 and 30 CH and a distilled-water control. After the treatments, the seeds were subjected to accelerated aging (48h at 42ºC in a growth chamber (25 ± 2ºC. The study evaluated the germination, the length of primary roots and shoots, the fresh weight of roots and shoots and the enzymatic activity of peroxidase (POX-EC1.11.1.7. The variables were analysed by ANOVA, and the means were compared using the Scott-Knott test (p = 0.05. The germination and the fresh weight of roots and shoots of the seedlings treated with Pulsatilla nigricans were higher than the water control, except that CH 30 did not significantly increase the fresh weight of shoots. The dinamisations 6, 24 and 30 CH produced a lower primary root length compared with the control. The dinamisations 12, 18 and 30 CH yielded a greater length of shoots. The total length of seedlings was reduced by the high dilutions 6 and 24 CH.O objetivo do presente trabalho foi de avaliar o efeito de altas diluições de Pulsatilla nigricans nas dinamizações 6, 12, 18, 24 e 30 CH, no vigor de sementes de soja submetidas ao envelhecimento acelerado. O experimento foi conduzido em delineamento inteiramente casualizado com 6 tratamentos e 10 repetições. Os tratamentos foram constituídos das dinamizações 6, 12, 18, 24 e 30 CH e testemunha com água destilada. Após aplicação dos tratamentos, as sementes foram submetidas ao teste de envelhecimento acelerado (48h a 42ºC, em câmara de crescimento (25 ± 2ºC. Avaliou-se a germinação, o comprimento da raiz primária e da parte aérea, massa fresca da raiz e da parte aérea e atividade enzimática da

  8. Approaches to the indirect evaluation of germination and vigour

    Directory of Open Access Journals (Sweden)

    Matthews S.

    1998-01-01

    Full Text Available In comparisons of six seed lots of different F1 hybrid cultivars of cauliflower with similarly high laboratory germinations (above 90% separation in germination was achieved after controlled deterioration (C.D. at 24% moisture content (m.c. and 45 0C for 24 hours. This measure of vigour was related to the position of the lots on the seed survival curve and was highly predictive of the longevity of the lots when stored at 15% m.c. and 20 0C for 12 and 16 weeks. When each seed lot was deteriorated at 24% m.c. for increasing times (from 0 to 36 hours a reduction in the subsequent percentage germination was seen, which, using probit transformed percentages, was significantly and linearly related to the leakage of electrolytes into seed soak water over 24 hours. The case is made for an approach to the indirect evaluation of germination and vigour using C.D. followed by measurements of leakage that could be more discerning and rapid than the present laboratory germination test.

  9. Seed germination and seedling vigour of italian ryegrass, cocksfoot and timothy following harvest and storage Germinação de sementes e o vigor de plantas jovens de azevem italiano, dactilis e timóteo após a colheita e o armazenamento

    Directory of Open Access Journals (Sweden)

    Rade Stanisavljevic

    2011-12-01

    Full Text Available During post-harvest maturation, different species vary in the length of dormancy breaking or germination increases. Seed dormancy and slow seedling development often limit establishment of forage grass stands. Seed germination and seedling vigour of Italian ryegrass (Lolium italicum A. Braun, Synonym Lolium multiflorum L., cocksfoot (Dactylis glomerata L. and timothy (Phleum pretense L. were observed after harvest and storage. After harvest in June, seeds were stored under standard storage conditions and sampled every 30 days after harvest (DAH, up to 270 DAH, and then every 60 days up to 990 DAH. At each date, seeds were tested for final germination percentage and for seedling vigour traits. Timothy seeds had a maximum germination (88% and the best seedlings vigour at 90 DAH, which implies that early autumn (September-October is the best sowing period for freshly harvested seeds of timothy. Timothy seed germination was poor from 270 DAH (73%. The best germination and vigour of Italian ryegrass and cocksfoot seedlings were between 270 and 330 DAH, which equates to spring sowing time (March-April in the succeeding year. Cocksfoot and Italian ryegrass seeds maintained satisfactory germination levels up to 630 DAH (81% and 810 DAH (81%, respectively. The data can serve for the determination of a proper storage duration management between harvest and sowing of the tested species under ambient conditions of south-eastern Europe.Durante o armazenamento, diferentes espécies comportam-se diferentemente quanto à dormência e crescimento de plântulas. A dormência o desenvolvimento lento das plântulas limitam a época do plantio das gramíneas forrageiras plurianuais. A germinação de sementes e o crescimento das plântulas de azevem italiano (Lolium italicum A. Braun sin. Lolium multiflorum L., Dactilis (Dactilis glomerata L. e de timoteo (Phleum pratense L. foi pesquisada durante a maturação pós-colheita e a silagem. Após a colheita, em junho

  10. Viability Prediction of Ricinus cummunis L. Seeds Using Multispectral Imaging

    DEFF Research Database (Denmark)

    Olesen, Merete Halkjær; Nikneshan, Pejman; Shrestha, Santosh

    2015-01-01

    The purpose of this study was to highlight the use of multispectral imaging in seed quality testing of castor seeds. Visually, 120 seeds were divided into three classes: yellow, grey and black seeds. Thereafter, images at 19 different wavelengths ranging from 375–970 nm were captured of all the s...

  11. Use of multispectral images and chemometrics in tomato seed studies

    DEFF Research Database (Denmark)

    Shrestha, Santosh; Deleuran, Lise Christina; Gislum, René

    During the production of tomato seeds, green tomatoes are normally discarded before seed extraction irrespective of their maturity stage. Studies indicate that seeds from green tomatoes may reach be able to reach full germination capacity. Thus the potential of multispectral imaging for non......-destructive discrimination of seeds based on their germination capacity was investigated. A total of 840 seeds extracted from green and red tomatoes were divided into two sets; a training set and a test set consisting of 648 and 192 seeds respectively. Each set consisted of 96 seeds from green tomatoes. The multispectral...... images of the seeds were captured and normalized canonical discriminant analysis was used to analyse the images. Germination tests were performed and seeds that subsequently germinated were recorded as viable. The viable seeds were classified with 99% and 98% accuracy for the training and test set...

  12. Soybean seed viability and changes of fatty acids content as ...

    African Journals Online (AJOL)

    The characteristics of soybean seed chemical composition are related to specific processes occurring in seed during storage. These changes lead to seed aging during storage and affect seed vigour and content of fatty acids. In order to reveal severity of their influence, the following vigour tests were applied: Standard ...

  13. Image fusion techniques in permanent seed implantation

    Directory of Open Access Journals (Sweden)

    Alfredo Polo

    2010-10-01

    image fusion for permanent seed implantation.

  14. INFLUENCE OF TEMPERATURE AND RELATIVE HUMIDITY ON GRAIN MOISTURE, GERMINATION AND VIGOUR OF THREE WHEAT CULTIVARS DURING ONE YEAR STORAGE

    Directory of Open Access Journals (Sweden)

    Ivica Strelec

    2010-12-01

    Full Text Available Changes in grain moisture, germination and vigour of three wheat cultivars packed in paper bags and stored for one year under four different conditions of environmental temperature and relative humidity (RH were investigated. During the first ninety days of storage significant reduction in grain moisture content of 4, 2.5 and 0.9 %, respectively, under 40 °C, 25 °C and 4 °C and RH of 45 % occurred. Subsequently grain moisture remained constant until the end of storage. Seeds of examined cultivars lost their germination ability and vigour only under elevated storage temperatures. Germination and vigour loss after one year of storage differed between cultivars being higher for seeds kept under 40°C, RH = 45% (35-85 % and 55-94 %, respectively, than under 25°C, RH = 45 % (10-20 % and 15-22 %, respectively. Obtained data indicate significant influence of storage conditions on moisture content, germination and vigour changes during storage of wheat seeds, as well as varietal dependence of seed viability.

  15. Viability and vigour of ageing winter wheat grains

    Directory of Open Access Journals (Sweden)

    Stanisław Grzesiuk

    2014-01-01

    Full Text Available The viability and vigour of ageing winter wheat caryopses of the cvs. Grana and Jana were tested. Viability was determined on the basis of germination capacity and rate, and vigour on the basis of the over-all activity of hydrogenases in the sprouts, exudate conductometry, analysis of sprout growth, oxygen uptake and mitochondrial protein content in the sprouts. What is called energy (or rate of germination and over-all dehydrogenase activity in embryos and sprouts and the electroconductivity of exudates were found to be very good measures of the vigour of ageing caryopses. The latter two indices of vigour should be determined at a strictly defined moment of swelling and germination. Good measures of caryopse vigour are also respiration during swelling and at the beginning of germination and mitochondrial protein content in the sprouts or seedlings. There is a high correlation between the vigour of ageing grain and its bioenergetic indices.

  16. Vigour Health: the ASC-Series launch in Canada

    OpenAIRE

    Danielsen, David M.; Fredriksen, Joakim; Nordli, Espen; Nøstberg, Bjørn R.

    2008-01-01

    This report is part of the authors’ bachelor degree in International Marketing and analyzes the Canadian vitamins and dietary supplements (VDS) industry. Based on this analysis, the authors will be proposing how Vigour can enter the market. Vigour Health was founded in 2006 by Omar Paulsen Bekheet and John Ivar Andre and the goal was to introduce a new lifestyle concept that should be inventive in its approach. Vigour utilizes raw materials from the northern parts of Norway, an...

  17. Teste de frio e envelhecimento acelerado na avaliação de vigor de sementes de feijão-frade Evaluation of the cowpea seeds vigour through of the coldtest and accelerated aging test

    Directory of Open Access Journals (Sweden)

    Josiane Marlle Guiscem

    2010-12-01

    Full Text Available Este trabalho teve como objetivo avaliar o vigor de sementes de feijão-caupi ou feijão-frade por meio dos testes de frio e de envelhecimento acelerado. Utilizou-se as seguintes cultivares de feijão-frade: BRS Guariba, BR 17 Gurguéia, BRS Marataoã, Quarentão e Vinagre. O experimento foi conduzido na Universidade Estadual do Maranhão onde se utilizou para o teste de envelhecimento acelerado as temperaturas e tempo de exposição: 41ºC, 42ºC 43ºC e 45ºC durante 48 h e 42ºC durante 72 h e para o teste de frio: 10ºC, 13ºC e 15ºC com 3, 4 e 5 dias de exposição. Os resultados revelaram que o teste de envelhecimento acelerado com 43ºC/48 h e para o de frio 10º /3 dias foram os mais adequados e que os testes de envelhecimento 42ºC /48 h e de frio 10ºC/3dias se correlacionaram entre si coeficiente de correlação de 0,97%. Pelos resultados conclui-se que os testes estudados apresentaram sensibilidade para identificar diferenças no vigor de sementes de feijão-frade.Aiming to evaluate the efficiency of different methods of accelerated aging and cold test to determine the physiological quality of seed of five cultivars of cowpea: BRS Guariba, BR 17 Gurguéia, BRS Marataoã, Quarentão e Vinagre. The experiment was conducted at the laboratory of University State of Maranhão - UEMA. In the accelera-ted aging test were used the following temperature and time of exposure: 41ºC, 42°C , 43°C and 45°C for 48 hours and 42 ° C for 72 hours. In cold test were used 10ºC, 13°C and 15ºC for 3, 4 and 5 days of exposure. Tests showed that the accelerated aging test of the combination of 43ºC/48 hours and for cold test the combination 15ºC/ 5 days were the most appropriate for the evaluation of the potential of physiological cultivars of cowpea seed. The accelerated aging tests 42°C/48 hours and of cold test 15ºC/3 days were those that had greater consistency in the separation of lots of cultivars of seed cowpea because of correlation

  18. Use of the software Seed Vigor Imaging System (SVIS® for assessing vigor of carrot seeds

    Directory of Open Access Journals (Sweden)

    José Luís de Marchi

    Full Text Available ABSTRACT Seed vigor has traditionally been evaluated by physiological, biochemical and stress tolerance tests. More recently, with the use of computerized image analysis, objective information has become accessible in a relatively short period of time, with less human interference. The aim of this study was to verify the efficiency of computerized seedling image analysis by Seed Vigor Imaging System (SVIS® to detect differences in vigor between carrot (Daucus carota L. seed lots as compared to those provided by traditional vigor tests. Seeds from seven lots from the Brasilia cultivar were subjected to a germination test, first count of germination, speed of germination, accelerated aging with saline solution and seedling emergence; furthermore, a vigor index, growth index and uniformity index were determined by the Seed Vigor Imaging System (SVIS® during four evaluation periods. The results obtained by the computerized seedling analysis (vigor index and growth index show that SVIS® is efficient in assessing carrot seed vigor.

  19. Vigour evaluation for genetics and breeding in rose

    NARCIS (Netherlands)

    Yan, Z.; Dolstra, O.; Hendriks, T.; Prins, T.W.; Stam, P.; Visser, P.B.

    2005-01-01

    Breeding of cut and pot rose cultivars for efficient production under low-energy conditions in greenhouses will be facilitated by understanding the inheritance of vigour. To get insight into the genetic variation of vigour-related traits, a diploid rose population was employed for an evaluation

  20. Genetics of early growth vigour in lentil (Lens culinaris Medik.)

    Indian Academy of Sciences (India)

    Rapid early growth vigour, 70–75 days to flowering. Figure 1. Frequency distributions of early growth vigour based on seedling length in parents (DPL15, ILL7663 and ILL6002) and F2 populations derived from two crosses (DPL15 × ILL7663; DPL15 × ILL6002) in lentil. 324. Journal of Genetics, Vol. 92, No. 2, August 2013 ...

  1. Imbibition of wheat seeds: Application of image analysis

    Science.gov (United States)

    Lev, Jakub; Blahovec, Jiří

    2017-10-01

    Image analysis is widely used for monitoring seeds during germination, and it is often the final phase of germination that is subjected to the greatest attention. However, the initial phase of germination (the so-called imbibition) also exhibits interesting behaviour. This work shows that image analysis has significant potential in the imbibition. Herein, a total of 120 seeds were analysed during germination tests, and information about seed size and shape was stored and analysed. It was found that the imbibition can be divided into two newly defined parts. The first one (`abrupt imbibition') consists mainly of the swelling of the seed embryo part and lasts approximately one hour. The second one, referred to as `main imbibition', consists mainly of spatial expansion caused by imbibition in the other parts of the seed. The results presented are supported by the development of seed cross area and shape parameters, and by direct observation.

  2. GARDEN CRESS GERMINABILITY AND SEEDLING VIGOUR AFTER TREATMENT WITH PLANT EXTRACTS

    Directory of Open Access Journals (Sweden)

    Miroslav Lisjak

    2015-12-01

    Full Text Available The usage of biologically active and environmentally friendly compounds has increasingly important role in the primary food production. This study was conducted in order to examine the impact of five commercial plant extracts on the seed vigour of garden cress (Lepidium sativum L.. The applied plant extracts significantly increased the germination. The highest fresh weight of seedlings, and also the lowest dry matter accumulation were observed in the treatment KE-plantasalva® without the sea salt addition. Equisetum extract inhibited the root elongation and resulted in the highest percentage of dry matter accumulated in seedlings, but also the lowest fresh weight.

  3. Image-Analysis Based on Seed Phenomics in Sesame

    Directory of Open Access Journals (Sweden)

    Prasad R.

    2014-10-01

    Full Text Available The seed coat (testa structure of twenty-three cultivated (Sesamum indicum L. and six wild sesame (s. occidentale Regel & Heer., S. mulayanum Nair, S. prostratum Retz., S. radiatum Schumach. & Thonn., S. angustifolium (Oliv. Engl. and S. schinzianum Asch germplasm was analyzed from digital and Scanning Electron Microscopy (SEM images with dedicated software using the descriptors for computer based seed image analysis to understand the diversity of seed morphometric traits, which later on can be extended to screen and evaluate improved genotypes of sesame. Seeds of wild sesame species could conveniently be distinguished from cultivated varieties based on shape and architectural analysis. Results indicated discrete ‘cut off values to identify definite shape and contour of seed for a desirable sesame genotype along with the con-ventional practice of selecting lighter colored testa.

  4. Effects of elevated CO2 and temperature on seed quality

    DEFF Research Database (Denmark)

    Hampton, John G; Boelt, Birte; Rolston, M P

    2013-01-01

    production on three seed quality components: seed mass, germination and seed vigour. In response to elevated CO2, seed mass has been reported to both increase and decrease in C3 plants, but not change in C4 plants. Increases are greater in legumes than non-legumes, and there is considerable variation among...... species. Seed mass increases may result in a decrease of seed nitrogen (N) concentration in non-legumes. Increasing temperature may decrease seed mass because of an accelerated growth rate and reduced seed filling duration, but lower seed mass does not necessarily reduce seed germination or vigour. Like...

  5. High speed measurement of corn seed viability using hyperspectral imaging

    Science.gov (United States)

    Ambrose, Ashabahebwa; Kandpal, Lalit Mohan; Kim, Moon S.; Lee, Wang-Hee; Cho, Byoung-Kwan

    2016-03-01

    Corn is one of the most cultivated crops all over world as food for humans as well as animals. Optimized agronomic practices and improved technological interventions during planting, harvesting and post-harvest handling are critical to improving the quantity and quality of corn production. Seed germination and vigor are the primary determinants of high yield notwithstanding any other factors that may play during the growth period. Seed viability may be lost during storage due to unfavorable conditions e.g. moisture content and temperatures, or physical damage during mechanical processing e.g. shelling, or over heating during drying. It is therefore vital for seed companies and farmers to test and ascertain seed viability to avoid losses of any kind. This study aimed at investigating the possibility of using hyperspectral imaging (HSI) technique to discriminate viable and nonviable corn seeds. A group of corn samples were heat treated by using microwave process while a group of seeds were kept as control group (untreated). The hyperspectral images of corn seeds of both groups were captured between 400 and 2500 nm wave range. Partial least squares discriminant analysis (PLS-DA) was built for the classification of aged (heat treated) and normal (untreated) corn seeds. The model showed highest classification accuracy of 97.6% (calibration) and 95.6% (prediction) in the SWIR region of the HSI. Furthermore, the PLS-DA and binary images were capable to provide the visual information of treated and untreated corn seeds. The overall results suggest that HSI technique is accurate for classification of viable and non-viable seeds with non-destructive manner.

  6. Contribution of seedling vigour and anoxia/hypoxia-responsive genes to submergence tolerance in Vietnamese lowland rice (Oryza sativa L.

    Directory of Open Access Journals (Sweden)

    Hien Thi Thu Vu

    2016-09-01

    Full Text Available A direct-seeded rice cultivation system has been widely adopted in Asian countries. Optimum germination and vigorous seedling growth under submergence are key traits for the practice of direct seeding. We studied the post-germination seedling vigour in Vietnamese lowland rice accessions based on three bio-parameters, shoot elongation growth under five-day submergence in water-filled test-tubes, seedling recovery rate five days after transferring submerged seedlings to pots with soil and seedling survival rate 21 days after sowing seeds in nursery beds and immediate incubation under submergence. A large diversity was found in seedling vigour thus estimated among the accessions. Significantly high correlations were observed among all three bio-parameters, verifying the contribution of seedling vigour to the manifestation of submergence tolerance at this critical stage of rice development. To examine the roles of anoxia/hypoxia-responsive genes, the expression of 17 candidate genes was studied by reverse transcription polymerase chain reaction (RT-PCR and compared between selected vigorous and non-vigorous groups of accessions. Transcripts of all but two genes showed marked accumulation in submerged seedlings. No differences, however, were found between the two contrasting groups. The observed common and coordinate expression of anoxia/hypoxia-induced genes suggests that they might assume roles in attaining baseline tolerance against submergence stress. It was also suggested that some unknown genetic factors are operating in determining cultivar/genotype-specific levels of submergence tolerance as assessed by post-germination seedling vigour.

  7. Image seedling analysis to evaluate tomato seed physiological potential

    Directory of Open Access Journals (Sweden)

    Vanessa Neumann Silva

    Full Text Available Computerized seedling image analysis are one of the most recently techniques to detect differences of vigor between seed lots. The aim of this study was verify the hability of computerized seedling image analysis by SVIS® to detect differences of vigor between tomato seed lots as information provided by traditionally vigor tests. Ten lots of tomato seeds, cultivar Santa Clara, were stored for 12 months in controlled environment at 20 ± 1 ºC and 45-50% of relative humidity of the air. The moisture content of the seeds was monitored and the physiological potential tested at 0, 6 and 12 months after storage, with germination test, first count of germination, traditional accelerated ageing and with saturated salt solution, electrical conductivity, seedling emergence and with seed vigor imaging system (SVIS®. A completely randomized experimental design was used with four replications. The parameters obtained by the computerized seedling analysis (seedling length and indexes of vigor and seedling growth with software SVIS® are efficient to detect differences between tomato seed lots of high and low vigor.

  8. A virtual seed file: the use of multispectral image analysis in the management of genebank seed accessions

    DEFF Research Database (Denmark)

    Adsetts Edberg Hansen, Michael; R. Hay, Fiona; Carstensen, Jens Michael

    2015-01-01

    We present a method for multispectral seed phenotyping as a fast and robust tool for managing genebank accessions. A multispectral vision system was used to take images of the seeds of 20 diverse varieties of rice (approximately 30 seeds for each variety). This was followed by extraction of feature...

  9. Seedling imaging analysis and traditional tests to assess okra seed vigor

    OpenAIRE

    Kikuti,Ana Lúcia Pereira; Marcos-Filho,Júlio

    2013-01-01

    Seed vigor testing is an important component of quality control programs adopted by seed industry. The software Seed Vigor Imaging System (SVIS) has been successfully used for seed vigor assessment in different species. The objective of this research was to verify the SVIS efficiency to assess okra seed vigor in comparison to other vigor tests used for this species. Five seed lots of 'Clemson Americano' and four of 'Santa Cruz' were submitted to germination (speed and percentage), cold germin...

  10. A role for seed storage proteins in Arabidopsis seed longevity

    NARCIS (Netherlands)

    Nguyen, Thu-Phuong|info:eu-repo/dai/nl/328228818; Cueff, Gwendal; Hegedus, Dwayne D; Rajjou, Loïc; Bentsink, Leónie|info:eu-repo/dai/nl/241338735

    2015-01-01

    Proteomics approaches have been a useful tool for determining the biological roles and functions of individual proteins and identifying the molecular mechanisms that govern seed germination, vigour and viability in response to ageing. In this work the dry seed proteome of four Arabidopsis thaliana

  11. Crop physiological analysis of seed quality variation in common bean (Phaseolus vulgaris L.)

    NARCIS (Netherlands)

    Muasya, R.M.

    2001-01-01

    Keywords : Physiological maturity, harvest maturity, earliness, common bean, Phaseolus vulgaris L., morphological markers, variation, moisture content, dry weight, viability, vigour, electrical conductivity, tetrazolium, seed lot, seed

  12. The Design and Development of Test Platform for Wheat Precision Seeding Based on Image Processing Techniques

    OpenAIRE

    Li , Qing; Lin , Haibo; Xiu , Yu-Feng; Wang , Ruixue; Yi , Chuijie

    2009-01-01

    International audience; The test platform of wheat precision seeding based on image processing techniques is designed to develop the wheat precision seed metering device with high efficiency and precision. Using image processing techniques, this platform gathers images of seeds (wheat) on the conveyer belt which are falling from seed metering device. Then these data are processed and analyzed to calculate the qualified rate, reseeding rate and leakage sowing rate, etc. This paper introduces t...

  13. Research on tomato seed vigor based on X-ray digital image

    Science.gov (United States)

    Zhao, Xueguan; Gao, Yuanyuan; Wang, Xiu; Li, Cuiling; Wang, Songlin; Feng, Qinghun

    2016-10-01

    Seed size, interior abnormal and damage of the tomato seeds will affect the germination. The purpose of this paper was to study the relationship between the internal morphology, seed size and seed germination of tomato. The preprocessing algorithm of X-ray image of tomato seeds was studied, and the internal structure characteristics of tomato seeds were extracted by image processing algorithm. By developing the image processing software, the cavity area between embryo and endosperm and the whole seed zone were determined. According to the difference of area of embryo and endosperm and Internal structural condition, seeds were divided into six categories, Respectively for three kinds of tomato seed germination test, the relationship between seed vigor and seed size , internal free cavity was explored through germination experiment. Through seedling evaluation test found that X-ray image analysis provide a perfect view of the inside part of the seed and seed morphology research methods. The larger the area of the endosperm and the embryo, the greater the probability of healthy seedlings sprout from the same size seeds. Mechanical damage adversely effects on seed germination, deterioration of tissue prone to produce week seedlings and abnormal seedlings.

  14. Automated matching of corresponding seed images of three simulator radiographs to allow 3D triangulation of implanted seeds

    Science.gov (United States)

    Altschuler, Martin D.; Kassaee, Alireza

    1997-02-01

    To match corresponding seed images in different radiographs so that the 3D seed locations can be triangulated automatically and without ambiguity requires (at least) three radiographs taken from different perspectives, and an algorithm that finds the proper permutations of the seed-image indices. Matching corresponding images in only two radiographs introduces inherent ambiguities which can be resolved only with the use of non-positional information obtained with intensive human effort. Matching images in three or more radiographs is an `NP (Non-determinant in Polynomial time)-complete' problem. Although the matching problem is fundamental, current methods for three-radiograph seed-image matching use `local' (seed-by-seed) methods that may lead to incorrect matchings. We describe a permutation-sampling method which not only gives good `global' (full permutation) matches for the NP-complete three-radiograph seed-matching problem, but also determines the reliability of the radiographic data themselves, namely, whether the patient moved in the interval between radiographic perspectives.

  15. Short-lag spatial coherence beamforming of photoacoustic images for enhanced visualization of prostate brachytherapy seeds

    OpenAIRE

    Lediju Bell, Muyinatu A.; Kuo, Nathanael; Song, Danny Y.; Boctor, Emad M.

    2013-01-01

    Prostate brachytherapy, administered by implanting tiny radioactive seeds to treat prostate cancer, currently relies on transrectal ultrasound imaging for intraoperative visualization of the metallic seeds. Photoacoustic (PA) imaging has been suggested as a feasible alternative to ultrasound imaging due to its superior sensitivity to metal surrounded by tissue. However, PA images suffer from poor contrast when seeds are distant from the light source. We propose a transperineal light delivery ...

  16. Investigation of radical locations in various sesame seeds by CW EPR and 9-GHz EPR imaging.

    Science.gov (United States)

    Nakagawa, K; Hara, H

    2015-01-01

    We investigated the location of radical in various sesame seeds using continuous-wave (CW) electron paramagnetic resonance (EPR) and 9-GHz EPR imaging. CW EPR detected persistent radicals (single line) for various sesame seeds. The EPR linewidth of black sesame seeds was narrower than that of the irradiated white sesame seeds. A very small signal was detected for the white sesame seeds. Two-dimensional (2D) imaging using a 9-GHz EPR imager showed that radical locations vary for various sesame seeds. The paramagnetic species in black sesame seeds were located on the seed coat (skin) and in the hilum region. The signal with the highest intensity was obtained from the hilum part. A very low-intensity image was observed for the white sesame seeds. In addition, the 2D imaging of the irradiated white sesame seeds showed that free radicals were located throughout the entire seed. For the first time, CW EPR and 9-GHz EPR imaging showed the exact location of radical species in various sesame seeds.

  17. Effect of seed treatment with milk powder and mustard flour in control of common bunt (Tilletia tritici) in wheat and stem smut (Urocystis occulta) in rye

    OpenAIRE

    Borgen, Anders; Kristensen, Lars

    2001-01-01

    In field trials mustard flour was able to control seed borne infection by common bunt (Tilletia tritici) in wheat without decreasing the germination vigour of the treated seeds. Full control of common bunt by coating the seeds with milk powder could only be achieved at doses which reduced germination vigour of the seeds. Mustard flour can be recommended as a seed treatment in organic agriculture while a treatment based on milk powder should be developed in combination with biological control....

  18. Acute serotonin depletion releases motivated inhibition of response vigour.

    Science.gov (United States)

    den Ouden, Hanneke E M; Swart, Jennifer C; Schmidt, Kristin; Fekkes, Durk; Geurts, Dirk E M; Cools, Roshan

    2015-04-01

    The neurotransmitter serotonin has long been implicated in the motivational control of behaviour. Recent theories propose that the role of serotonin can be understood in terms of an interaction between a motivational and a behavioural activation axis. Experimental support for these ideas, however, has been mixed. In the current study, we aimed to investigate the role of serotonin (5HT) in behavioural vigour as a function of incentive motivation. We employed dietary acute tryptophan depletion (ATD) to lower the 5HT precursor tryptophan during the performance of a speeded visual discrimination task. Feedback valence and feedback probability were manipulated independently and cued prior to target onset. On feedback trials, fast correct responses led to either reward or avoidance of punishment, while slow or incorrect responses led to reward omission or punishment. We show that behavioural responding is inhibited under high incentive motivation (i.e. high-feedback probability) at baseline 5HT levels and that lowering these leads to behavioural disinhibition, while leaving accuracy unaffected. Surprisingly, there were no differential effects of motivational valence, with 5HT depletion releasing behavioural inhibition under both appetitive and aversive motivation. Our findings extend current theories on the role of 5HT in behavioural inhibition by showing that reductions in serotonin lead to increased behavioural vigour only if there is a motivational drive to inhibit behaviour at baseline.

  19. Raman Hyperspectral Imaging for Detection of Watermelon Seeds Infected with Acidovorax citrulli

    OpenAIRE

    Lee, Hoonsoo; Kim, Moon S.; Qin, Jianwei; Park, Eunsoo; Song, Yu-Rim; Oh, Chang-Sik; Cho, Byoung-Kwan

    2017-01-01

    The bacterial infection of seeds is one of the most important quality factors affecting yield. Conventional detection methods for bacteria-infected seeds, such as biological, serological, and molecular tests, are not feasible since they require expensive equipment, and furthermore, the testing processes are also time-consuming. In this study, we use the Raman hyperspectral imaging technique to distinguish bacteria-infected seeds from healthy seeds as a rapid, accurate, and non-destructive det...

  20. Treatments for improving seed germination in eggplant and related species

    OpenAIRE

    Gisbert, Carmina; Prohens, J.; Nuez, Fernando

    2011-01-01

    7 pages, 2 figures, 1 table, 19 references. Trabajo incluido en el Capítulo: Seed Genetics and Biotechnology, an Seed Germination and Vigour, presentado al Fith International Symposium on Seed, Transplant and Stand Establishment of Horticultural Crops, celebrado del 27/09 al 1/10 de 2009, en Murcia y Almería, España.

  1. Multispectral fluorescence imaging technique for discrimination of cucumber (Cucumis Sativus) seed viability

    Science.gov (United States)

    In this study, we developed a nondestructive method for discriminating viable cucumber (Cucumis sativus) seeds based on hyperspectral fluorescence imaging. The fluorescence spectra of cucumber seeds in the 420–700 nm range were extracted from hyperspectral fluorescence images obtained using 365 nm u...

  2. A study on image reconstruction for seed localization for permanent prostate brachytherapy

    International Nuclear Information System (INIS)

    Hong, Ju Young; Rah, Jeong Eun; Suh, Tae Suk

    2007-01-01

    This study was to design and fabricate a phantom for prostate cancer brachytherapy to validate a developed program applying a 3-film technique, and to compare it with the conventional 2-film technique for determining the location of an implanted seed. The images were obtained from overlapped seeds by randomly placing a maximum of 63 seeds in the interior-posterior (AP) position and at -30 .deg. to 30 .deg. at 15 .deg. intervals. Images obtained by use of the phantom were applied to the image processing procedure, and were then processed into the development program for seed localization. In this study, cases were set where one seed overlapped, where two seeds overlapped and where none of the three views resolved all seeds. The distance between the centers of each seed to the reference seed was calculated in a prescribed region. This distance determined the location of each seed in a given band. The location of the overlapped seeds was compared with that of the 2-film technique. With this program, the detection rate was 92.2% (at ± 15 .deg. ), 94.1% (at ± 30 .deg.) and 70.6% (compared to the use of the 2-film technique). The overlaps were caused by one or more than two seeds that overlapped; the developed program can identify the location of each seed perfectly. However, for the third case the program was not able to resolve the overlap of the seeds. This program can be used to improve treatment outcome for the brachytherapy of prostate cancer by reducing the number of errors in the process of reconstructing the locations of perfectly overlapped seeds

  3. Automatic seed picking for brachytherapy postimplant validation with 3D CT images.

    Science.gov (United States)

    Zhang, Guobin; Sun, Qiyuan; Jiang, Shan; Yang, Zhiyong; Ma, Xiaodong; Jiang, Haisong

    2017-11-01

    Postimplant validation is an indispensable part in the brachytherapy technique. It provides the necessary feedback to ensure the quality of operation. The ability to pick implanted seed relates directly to the accuracy of validation. To address it, an automatic approach is proposed for picking implanted brachytherapy seeds in 3D CT images. In order to pick seed configuration (location and orientation) efficiently, the approach starts with the segmentation of seed from CT images using a thresholding filter which based on gray-level histogram. Through the process of filtering and denoising, the touching seed and single seed are classified. The true novelty of this approach is found in the application of the canny edge detection and improved concave points matching algorithm to separate touching seeds. Through the computation of image moments, the seed configuration can be determined efficiently. Finally, two different experiments are designed to verify the performance of the proposed approach: (1) physical phantom with 60 model seeds, and (2) patient data with 16 cases. Through assessment of validated results by a medical physicist, the proposed method exhibited promising results. Experiment on phantom demonstrates that the error of seed location and orientation is within ([Formula: see text]) mm and ([Formula: see text])[Formula: see text], respectively. In addition, the most seed location and orientation error is controlled within 0.8 mm and 3.5[Formula: see text] in all cases, respectively. The average process time of seed picking is 8.7 s per 100 seeds. In this paper, an automatic, efficient and robust approach, performed on CT images, is proposed to determine the implanted seed location as well as orientation in a 3D workspace. Through the experiments with phantom and patient data, this approach also successfully exhibits good performance.

  4. Pepper seed germination assessed by combined X-radiography and computer-aided imaging analysis

    International Nuclear Information System (INIS)

    Dell'Aquila, A.

    2007-01-01

    A lot of pepper seeds having 87% germination were subjected to X-ray inspection using a non lethal dose of radiation. Seeds with less than 2.7% (on the basis of total seed area) of free space area, i.e. the spaces between embryo and endosperm, were classified as highly viable seeds (97-100% germination) with the lowest level of abnormal seedlings. Seeds X-ray classified as good were subjected to a computerised image analysis to study seed imbibition and radicle elongation. The patterns of seed area increase, chosen as the most accurate indicator of seed swelling, resembled the triphasic curve of water uptake. The first phase was completed at 9 h followed by a second phase that varied widely in time until completion of germination between 52 and 96 h. The proportion of seeds with radicle protrusion between 52-56 h and 64-72 h assessed with the image analysis was significantly higher than that recorded using a conventional germination test. In addition, the rate of increase of seed area during the third phase of imbibition, mostly due to protrusion of the radicle tip and its growth, was highly correlated with the corresponding radicle elongation rate

  5. seeds

    African Journals Online (AJOL)

    Owner

    peptidohydrolase (8.0%) from mung bean seedlings. (Baumgartner and Chrispeels, 1977), EP-HG (4.5%) from horse gram seedlings ( Rajeswari, 1997), acidic protease (15%) from germinating winged-bean seeds. (Usha and Singh, 1996) and EP-1 (1.6%) from barley seedlings and GA3-induced cysteine protease (3.38%).

  6. Demonstration of Clean Particle Seeding for Particle Image Velocimetry in a Closed Circuit Supersonic Wind Tunnel

    National Research Council Canada - National Science Library

    McNiel, Charles M

    2007-01-01

    The purpose of this research was to determine whether solid carbon dioxide (CO2) particles might provide a satisfactory, and cleaner, alternative to traditional seed material for Particle Image Velocimetry (PIV...

  7. Multispectral imaging as a potential tool for seed health testing of spinach (Spinacia oleracea L.)

    DEFF Research Database (Denmark)

    Olesen, M. Halkjaer; Carstensen, Jens Michael; Boelt, B.

    2011-01-01

    Seed health tests are time consuming and require substantial training for characterization of pathogenic fungi on seed. A new approach to use a multispectral vision system for identifying surface properties of different fungal infections has been tested in spinach (Spinacia oleracea L.) at Aarhus...... University. Our study demonstrates that multispectral imaging with wavelengths ranging from 395-970 nm can be used to distinguish between uninfected spinach seeds and seeds infected with Verticillium spp., Fusarium spp., Stemphylium botryosum, Cladosporium spp. and Alternaria alternata. Analytical separation...... based on mean pixel intensity, Canonical Discriminant Analysis (CDA) and classification by Jeffries-Matusita (JM) distance illustrates that a combination of Near Infrared spectra (NIR) and Visual spectra (VIS) is able to identify uninfected seeds from infected seeds ranging from 80-100%. Classification...

  8. Plant response to sunflower seeds to osmotic conditioning

    Directory of Open Access Journals (Sweden)

    Camila Santos Barros de Morais

    2014-10-01

    Full Text Available The aim of this study was to evaluate the effect of seeds osmotic conditioning in seedlings emergence and plants performance of sunflower. Three lots of seeds sunflower (Catissol, was submited to osmotic conditioning with polyethylene glycol solution, –2,0 MPa in aerated system, under 15 ºC for 8 hour and then was evaluated for germination tests and vigour. Under filed conditions was conducted emergency evaluations of seedling, plants development as well as the productivity and seeds quality, and the accumulation of nutrients in the seeds. The osmotic conditioning improve the survival of seedling, the dry matter mass to aerial part of plants from 60 days after sowing and oil content, in lots with low seeds physiological quality. The osmotic conditioning not increase the seeds yield but promotes the vigour of seeds produced, regardless of the lot used for sowing seeds.

  9. Raman Hyperspectral Imaging for Detection of Watermelon Seeds Infected with Acidovorax citrulli.

    Science.gov (United States)

    Lee, Hoonsoo; Kim, Moon S; Qin, Jianwei; Park, Eunsoo; Song, Yu-Rim; Oh, Chang-Sik; Cho, Byoung-Kwan

    2017-09-23

    The bacterial infection of seeds is one of the most important quality factors affecting yield. Conventional detection methods for bacteria-infected seeds, such as biological, serological, and molecular tests, are not feasible since they require expensive equipment, and furthermore, the testing processes are also time-consuming. In this study, we use the Raman hyperspectral imaging technique to distinguish bacteria-infected seeds from healthy seeds as a rapid, accurate, and non-destructive detection tool. We utilize Raman hyperspectral imaging data in the spectral range of 400-1800 cm -1 to determine the optimal band-ratio for the discrimination of watermelon seeds infected by the bacteria Acidovorax citrulli using ANOVA. Two bands at 1076.8 cm -1 and 437 cm -1 are selected as the optimal Raman peaks for the detection of bacteria-infected seeds. The results demonstrate that the Raman hyperspectral imaging technique has a good potential for the detection of bacteria-infected watermelon seeds and that it could form a suitable alternative to conventional methods.

  10. BrachyView: Combining LDR seed positions with transrectal ultrasound imaging in a prostate gel phantom.

    Science.gov (United States)

    Alnaghy, S; Cutajar, D L; Bucci, J A; Enari, K; Safavi-Naeini, M; Favoino, M; Tartaglia, M; Carriero, F; Jakubek, J; Pospisil, S; Lerch, M; Rosenfeld, A B; Petasecca, M

    2017-02-01

    BrachyView is a novel in-body imaging system which aims to provide LDR brachytherapy seeds position reconstruction within the prostate in real-time. The first prototype is presented in this study: the probe consists of a gamma camera featuring three single cone pinhole collimators embedded in a tungsten tube, above three, high resolution pixelated detectors (Timepix). The prostate was imaged with a TRUS system using a sagittal crystal with a 2.5mm slice thickness. Eleven needles containing a total of thirty 0.508U 125 I seeds were implanted under ultrasound guidance. A CT scan was used to localise the seed positions, as well as provide a reference when performing the image co-registration between the BrachyView coordinate system and the TRUS coordinate system. An in-house visualisation software interface was developed to provide a quantitative 3D reconstructed prostate based on the TRUS images and co-registered with the LDR seeds in situ. A rigid body image registration was performed between the BrachyView and TRUS systems, with the BrachyView and CT-derived source locations compared. The reconstructed seed positions determined by the BrachyView probe showed a maximum discrepancy of 1.78mm, with 75% of the seeds reconstructed within 1mm of their nominal location. An accurate co-registration between the BrachyView and TRUS coordinate system was established. The BrachyView system has shown its ability to reconstruct all implanted LDR seeds within a tissue equivalent prostate gel phantom, providing both anatomical and seed position information in a single interface. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

  11. Assessment of pesticide coating on cereal seeds by near infrared hyperspectral imaging

    Directory of Open Access Journals (Sweden)

    Ph. Vermeulen

    2017-01-01

    Full Text Available Classical chromatographic methods, such as ultra performance liquid chromatography (UPLC, are used as reference methods to assess seed quality and homogeneous pesticide coating of seeds. These methods have some important drawbacks since they are time consuming, expensive, destructive and require a substantial amount of solvent, among others. Near infrared (NIR spectroscopy seems to be an interesting alternative technique for the determination of the quality of seed treatment and avoids most of these drawbacks. The objective of this study was to assess the quality of pesticide coating treatment by near infrared hyperspectral imaging (NIR-HSI by analysing, on a seed-by-seed basis, several seeds simultaneously in comparison to NIR spectroscopy and UPLC as the reference method. To achieve this goal, discrimination—partial least squares discriminant analysis (PLS-DA—models and regression—partial least squares (PLS—models were developed. The results obtained by NIR-HSI are compared to the results obtained with NIR spectroscopy and UPLC instruments. This study has shown the potential of NIR hyperspectral imaging to assess the quality/homogeneity of the pesticide coating on seeds.

  12. Image analysis of moving seeds in an indented cylinder

    DEFF Research Database (Denmark)

    Buus, Ole; Jørgensen, Johannes Ravn

    2010-01-01

    inspection in seed cleaning equipment. A prototype of an indented cylinder will be constructed. To make it more dynamic, the cylinder itself will be manufactured using 3D printing technology. The input will come either from 3D scans of existing cylinders or by defining their topology using parametric B...

  13. Multispectral imaging as a potential tool for seed health testing of spinach (Spinacia oleracea L.)

    DEFF Research Database (Denmark)

    Olesen, M. Halkjaer; Carstensen, Jens Michael; Boelt, B.

    2011-01-01

    University. Our study demonstrates that multispectral imaging with wavelengths ranging from 395-970 nm can be used to distinguish between uninfected spinach seeds and seeds infected with Verticillium spp., Fusarium spp., Stemphylium botryosum, Cladosporium spp. and Alternaria alternata. Analytical separation...... using only NIR gave a separation of 26-88% between uninfected and Fusarium spp. infected seeds. Alternaria alternata and Fusarium spp. could be distinguished from each other and from Cladosporium spp., Verticillium spp. and Stemphylium spp. Separation of Cladosporium spp., Verticillium spp....... and Stemphylium spp. needs further development before practical application....

  14. Development of non-destructive sorting technique for viability of watermelon seed by using hyperspectral image processing

    Energy Technology Data Exchange (ETDEWEB)

    Bae, Hyun Jin; Seo, Young Wook; Lohumi, Santosh; Park, Eun Soo; Cho, Byoung Kwan [Biosystems Machinery Engineering, Chungnam National University, Daejeon (Korea, Republic of); Kim, Dae Yong [Logistics institude, CJ Korea Express, Seoul (Korea, Republic of)

    2016-02-15

    Seed viability is one of the most important parameters that is directly related with seed germination performance and seedling emergence. In this study, a hyperspectral imaging (HSI) system having a range of 1000 –2500 nm was used to classify viable watermelon seeds from nonviable seeds. In order to obtain nonviable watermelon seeds, a total of 96 seeds were artificially aged by immersing the seeds in hot water (25°C) for 15 days. Further, hyperspectral images for 192 seeds (96 normal and 96 aged) were acquired using the developed HSI system. A germination test was performed for all the 192 seeds in order to confirm their viability. Spectral data from the hyperspectral images of the seeds were extracted by selecting pixels from the region of interest. Each seed spectrum was averaged and preprocessed to develop a classification model of partial least square discriminant analysis (PLS-DA). The developed PLS-DA model showed a classification accuracy of 94.7% for the calibration set, and 84.2% for the validation set. The results demonstrate that the proposed technique can classify viable and nonviable watermelon seeds with a reasonable accuracy, and can be further converted into an online sorting system for rapid and nondestructive classification of watermelon seeds with regard to viability.

  15. Development of non-destructive sorting technique for viability of watermelon seed by using hyperspectral image processing

    International Nuclear Information System (INIS)

    Bae, Hyun Jin; Seo, Young Wook; Lohumi, Santosh; Park, Eun Soo; Cho, Byoung Kwan; Kim, Dae Yong

    2016-01-01

    Seed viability is one of the most important parameters that is directly related with seed germination performance and seedling emergence. In this study, a hyperspectral imaging (HSI) system having a range of 1000 –2500 nm was used to classify viable watermelon seeds from nonviable seeds. In order to obtain nonviable watermelon seeds, a total of 96 seeds were artificially aged by immersing the seeds in hot water (25°C) for 15 days. Further, hyperspectral images for 192 seeds (96 normal and 96 aged) were acquired using the developed HSI system. A germination test was performed for all the 192 seeds in order to confirm their viability. Spectral data from the hyperspectral images of the seeds were extracted by selecting pixels from the region of interest. Each seed spectrum was averaged and preprocessed to develop a classification model of partial least square discriminant analysis (PLS-DA). The developed PLS-DA model showed a classification accuracy of 94.7% for the calibration set, and 84.2% for the validation set. The results demonstrate that the proposed technique can classify viable and nonviable watermelon seeds with a reasonable accuracy, and can be further converted into an online sorting system for rapid and nondestructive classification of watermelon seeds with regard to viability

  16. Non-destructive evaluation of bacteria-infected watermelon seeds using visible/near-infrared hyperspectral imaging.

    Science.gov (United States)

    Lee, Hoonsoo; Kim, Moon S; Song, Yu-Rim; Oh, Chang-Sik; Lim, Hyoun-Sub; Lee, Wang-Hee; Kang, Jum-Soon; Cho, Byoung-Kwan

    2017-03-01

    There is a need to minimize economic damage by sorting infected seeds from healthy seeds before seeding. However, current methods of detecting infected seeds, such as seedling grow-out, enzyme-linked immunosorbent assays, the polymerase chain reaction (PCR) and the real-time PCR have a critical drawbacks in that they are time-consuming, labor-intensive and destructive procedures. The present study aimed to evaluate the potential of visible/near-infrared (Vis/NIR) hyperspectral imaging system for detecting bacteria-infected watermelon seeds. A hyperspectral Vis/NIR reflectance imaging system (spectral region of 400-1000 nm) was constructed to obtain hyperspectral reflectance images for 336 bacteria-infected watermelon seeds, which were then subjected to partial least square discriminant analysis (PLS-DA) and a least-squares support vector machine (LS-SVM) to classify bacteria-infected watermelon seeds from healthy watermelon seeds. The developed system detected bacteria-infected watermelon seeds with an accuracy > 90% (PLS-DA: 91.7%, LS-SVM: 90.5%), suggesting that the Vis/NIR hyperspectral imaging system is effective for quarantining bacteria-infected watermelon seeds. The results of the present study show that it is possible to use the Vis/NIR hyperspectral imaging system for detecting bacteria-infected watermelon seeds. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  17. Sample Preparation of Corn Seed Tissue to Prevent Analyte Relocations for Mass Spectrometry Imaging.

    Science.gov (United States)

    Kim, Shin Hye; Kim, Jeongkwon; Lee, Young Jin; Lee, Tae Geol; Yoon, Sohee

    2017-08-01

    Corn seed tissue sections were prepared by the tape support method using an adhesive tape, and mass spectrometry imaging (MSI) was performed. The effect of heat generated during sample preparation was investigated by time-of-flight secondary mass spectrometry (TOF-SIMS) imaging of corn seed tissue prepared by the tape support and the thaw-mounted methods. Unlike thaw-mounted sample preparation, the tape support method does not cause imaging distortion because of the absence of heat, which can cause migration of the analytes on the sample. By applying the tape-support method, the corn seed tissue was prepared without structural damage and MSI with accurate spatial information of analytes was successfully performed. Graphical Abstract ᅟ.

  18. Sample Preparation of Corn Seed Tissue to Prevent Analyte Relocations for Mass Spectrometry Imaging

    Science.gov (United States)

    Kim, Shin Hye; Kim, Jeongkwon; Lee, Young Jin; Lee, Tae Geol; Yoon, Sohee

    2017-08-01

    Corn seed tissue sections were prepared by the tape support method using an adhesive tape, and mass spectrometry imaging (MSI) was performed. The effect of heat generated during sample preparation was investigated by time-of-flight secondary mass spectrometry (TOF-SIMS) imaging of corn seed tissue prepared by the tape support and the thaw-mounted methods. Unlike thaw-mounted sample preparation, the tape support method does not cause imaging distortion because of the absence of heat, which can cause migration of the analytes on the sample. By applying the tape-support method, the corn seed tissue was prepared without structural damage and MSI with accurate spatial information of analytes was successfully performed.

  19. Measurement of the density profile of pure and seeded molecular beams by femtosecond ion imaging

    Energy Technology Data Exchange (ETDEWEB)

    Meng, Congsen [LaserLaB Amsterdam, VU University Amsterdam, de Boelelaan 1083, 1081 HV Amsterdam (Netherlands); Department of Physics, National University of Defense Technology, Changsha 410073 (China); Janssen, Maurice H. M. [LaserLaB Amsterdam, VU University Amsterdam, de Boelelaan 1083, 1081 HV Amsterdam (Netherlands)

    2015-02-15

    Here, we report on femtosecond ion imaging experiments to measure the density profile of a pulsed supersonic molecular beam. Ion images are measured for both a molecular beam and bulk gas under identical experimental conditions via femtosecond multiphoton ionization of Xe atoms. We report the density profile of the molecular beam, and the measured absolute density is compared with theoretical calculations of the centre line beam density. Subsequently, we discuss reasons accounting for the differences between measurements and calculations and propose that strong skimmer interference is the most probable cause for the differences. Furthermore, we report on experiments measuring the centre line density of seeded supersonic beams. The femtosecond ion images show that seeding the heavy Xe atom at low relative seed fractions (1%-10%) in a light carrier gas like Ne results in strong relative enhancements of up to two orders of magnitude.

  20. Simplified quality control of radioactive seeds in sterile cartridge for prostate brachytherapy using an imaging plate and a collimator

    International Nuclear Information System (INIS)

    Saze, T.; Miyoshi, H.; Maezawa, H.; Kubota, M.; Furutani, S.; Nishitani, H.; Kawaguchi, Y.; Nakayama, S.; Ito, S.; Nishizawa, K.

    2008-01-01

    Image analyzer system and collimator has been successfully applied to calibrate simultaneously multiple 125 I seeds in a sterile cartridge. Seeds within the cartridge were placed on an imaging plate, and the imaging plate irradiated. To remove scatter radiation, and improve spatial resolution of seed images, this study used a specially designed collimator. The irradiated imaging plate was scanned using an image analyzer system, and radioactivity intensities of seed images were given in counts. Counts could be translated to profiles, and each seed within the cartridge was analyzed. It is observed that a good correlation between counts and total radioactivity of the seeds within the cartridge. Thus, using a least-squares line, it was possible to calibrate a seed with unknown apparent activity. By analyzing the profiles, it was possible not only to detect a mis calibrated seed in the cartridge from its relative difference in counts, but also to identify its position in the cartridge. Using an imaging analyzer system, all seeds in a cartridge could be calibrated in a sterile environment. (author)

  1. Alleviate Seed Ageing Effects in Silybum marianum by Application of Hormone Seed Priming

    Directory of Open Access Journals (Sweden)

    Seyed Ata SIADAT

    2015-09-01

    Full Text Available Most of the medicinal plants have seed dormancy or poor seed germination. This is due to their natural compounds or their morphological structure. Existence of such mechanisms makes the seeds able to endure harsh environments, stressful conditions or natural ageing. Different seed enhancement treatments were proposed in order to improve seed germination. In this study, it was examined Silybum marianum seed longevity (0, 48 and 72 hour of ageing and the response to seed priming when using different concentrations of cytokinin (50, 200, 350 and 500 ppm and different duration of treatment (8, 12 and 24 hour. Results revealed that ageing could be successfully alleviated using seed priming. Seed ageing significantly affected seed germination and vigour. Application of cytokinin considerably improved seed vigour in aged and non aged seeds. The most effective seed priming treatment was with 500 ppm cytokine, with the duration of 24 hours. In conclusion, it can be recommend the used of plant growth regulators like cytokine, as a good priming agent, to recover losses of seed quality and improve germination characteristics.

  2. Characterization biometric and morphophysiological of Peach rootstock seeds using images of their seedling vigor.

    Science.gov (United States)

    Souza, Aline Das Graças; Smiderle, Oscar Jose; Bianchi, Valmor Joao

    2018-04-26

    This study aimed to evaluate the efficiency of using the computerized imaging seed analysis system (SAS) in the biometric and morphophysiological characterization of seeds and the initial growth of seedlings from peach rootstocks. The experimental design was completely randomized with five replicates of 20 seeds. The variables analyzed were degree of seed humidity, length and width of seeds measured by SAS technology and manual measurements, mean germination time, germination percentage, radicle length and width, taproot length, length of the aerial part and taproot/aerial part ratio. The highest seed length, germination percentage (100%) and lower germination time (11.3), were obtained with the cv. Capdeboscq while, 'Tsukuba 1', 2' and 3' had intermediate seedlings length, varying from 1.55 to 1.65 cm with mean germination times between 14.5 and 18.0 days and average germination percentage of 96%. The computerized analysis of images is fast and efficient for biometric evaluations such as seed width and length, as well as initial growth of peach tree seedlings. The cvs Capdeboscq, Flordaguard and Tsukuba 2 presented greater radicle width, length and a mean taproot/aerial part ratio equal to 2, as well as higher number of adventitious roots, which indicated a strong positive correlation between radicle length, taproot length and initial seedling growth. The continuity of the research will certainly allow the development of reliable procedures for other species, besides allowing the identification of wider alternatives for the use of this system for the expansion of knowledge in the areas of physiology and evaluation of the physiological potential of seeds. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  3. The vigour of glasshouse roses : scion - rootstock relationships : effects of phenotypic and genotypic variation

    NARCIS (Netherlands)

    Vries, de D.P.

    1993-01-01

    Glasshouse roses commonly are combination plants, consisting of a scion variety and a rootstock of different genotypes. In this study, various environmental and genotypic factors have been investigated that influence the vigour of rootstocks and scion varieties, separately and in graft

  4. Strategies to control tree vigour and optimise fruit production in 'Conference' pears

    NARCIS (Netherlands)

    Maas, F.M.

    2008-01-01

    The ban on the use of chlormequat (CCC) in pear orchards in 2001 forced Dutch pear growers to look for alternative methods to control tree vigour and stimulate flower bud development and fruit production. Root pruning and trunk notching have become the major growth retarding methods. In addition to

  5. Irritable bowel syndrome and symptom severity: evidence of negative attention bias, diminished vigour, and autonomic dysregulation.

    Science.gov (United States)

    Phillips, Kristy; Wright, Bradley J; Kent, Stephen

    2014-07-01

    To determine if cognitive processing, and subjective and physiological responses to stress and relaxation differed between an irritable bowel syndrome (IBS) group and control group. How these variables relate to the severity of IBS symptoms was also determined. Twenty-one IBS participants and 20 controls provided cognitive (attention and processing), subjective (perceived stress and vigour), and physiological (heart rate, blood pressure, and skin conductance) data during a relaxation and stress phase. Logistic regression analyses determined which variables are related to the IBS group and hierarchical linear regression assessed how the variables are related to the severity of IBS symptoms. Subjective and cognitive factors (drowsiness at baseline, total vigour, and reduced Stroop colour-naming accuracy for negative words) are significantly related to IBS, χ2 (3, N=41)=23.67, pself-schema, as well as perceived low vigour were important in categorising IBS. Low subjective vigour and reduced physiological reactivity to both relaxation and stress conditions were associated with IBS severity, suggestive of illness-related allostatic load. Copyright © 2014 Elsevier Inc. All rights reserved.

  6. Non-destructive quality evaluation of pepper (Capsicum annuum L.) seeds using LED-induced hyperspectral reflectance imaging

    Science.gov (United States)

    In this study, we develop a viability evaluation method for pepper (Capsicum annuum L.) seed based on hyperspectral reflectance imaging. The reflectance spectra of pepper seeds in the 400–700 nm range are collected from hyperspectral reflectance images obtained using blue, green, and red LED illumin...

  7. Electron beam irradiation: laboratory and field studies of cowpea seeds

    International Nuclear Information System (INIS)

    Srinivasan, K.; Chauhan, S.K.; Prasad, T.V.; Pramod, R.; Verma, V.P.; Petwal, V.; Dwivedi, J.; Bhalla, S.

    2015-01-01

    Cowpea (Vigna unguiculata) rich in protein and vitamins is emerging as one of the most important food legumes to tackle malnutrition. Pulse beetles (Callosobruchus chinensis and C. maculatus) are the pests of economic importance causing enormous losses during storage. Although various pest management strategies exist for the control of these pests, environmental concerns necessitate developing ecofriendly strategies. Electron beam (EB) irradiation has the potential to be a viable, non-chemical, residue-free strategy for management of pulse beetles during storage, but higher doses affect seed germination and viability. Hence, the present investigation was taken up to analyse the dosage effect of the irradiation on seed attributes of cowpea. Healthy cowpea seeds were irradiated with low energy electrons at different doses viz., 180, 360, 540, 720, 900, 1080, 1260, 1440 and 1620 Gy at 500 keV using the EB Accelerator facility at Raja Ramanna Centre for Advanced Technology, Indore. EB irradiated seeds were tested for physiological viz., germination, seedling vigour and vigour index and biochemical parameters viz., electrical conductivity of seed leachate, seed viability/tetrazolium test and dehydrogenase activity. Germination and vigour of the irradiated seeds were evaluated as per the ISTA Rules (ISTA, 1996). Vigour index was calculated as the product of germination percentage and seedling vigour. About 3,000 irradiated seeds from each dose were grown in the field at the Experimental farm, National Bureau of Plant Genetic Resources, New Delhi. Seeds harvested from 1500 individual plants of M 1 generation from each dose (50 seeds from each plant individually) were sown in next season and observed for chlorophyll mutations, if any. Results revealed that doses upto 1080 Gy (88%) did not affect the germination of cowpea seeds drastically as compared to untreated seeds (98%). Lower doses viz., 180 and 360 Gy had no impact on vigour components while higher doses (1080 Gy

  8. Using hyperspectral imaging to determine germination of native Australian plant seeds.

    Science.gov (United States)

    Nansen, Christian; Zhao, Genpin; Dakin, Nicole; Zhao, Chunhui; Turner, Shane R

    2015-04-01

    We investigated the ability to accurately and non-destructively determine the germination of three native Australian tree species, Acacia cowleana Tate (Fabaceae), Banksia prionotes L.F. (Proteaceae), and Corymbia calophylla (Lindl.) K.D. Hill & L.A.S. Johnson (Myrtaceae) based on hyperspectral imaging data. While similar studies have been conducted on agricultural and horticultural seeds, we are unaware of any published studies involving reflectance-based assessments of the germination of tree seeds. Hyperspectral imaging data (110 narrow spectral bands from 423.6nm to 878.9nm) were acquired of individual seeds after 0, 1, 2, 5, 10, 20, 30, and 50days of standardized rapid ageing. At each time point, seeds were subjected to hyperspectral imaging to obtain reflectance profiles from individual seeds. A standard germination test was performed, and we predicted that loss of germination was associated with a significant change in seed coat reflectance profiles. Forward linear discriminant analysis (LDA) was used to select the 10 spectral bands with the highest contribution to classifications of the three species. In all species, germination decreased from over 90% to below 20% in about 10-30days of experimental ageing. P50 values (equal to 50% germination) for each species were 19.3 (A. cowleana), 7.0 (B. prionotes) and 22.9 (C. calophylla) days. Based on independent validation of classifications of hyperspectral imaging data, we found that germination of Acacia and Corymbia seeds could be classified with over 85% accuracy, while it was about 80% for Banksia seeds. The selected spectral bands in each LDA-based classification were located near known pigment peaks involved in photosynthesis and/or near spectral bands used in published indices to predict chlorophyll or nitrogen content in leaves. The results suggested that seed germination may be successfully classified (predicted) based on reflectance in narrow spectral bands associated with the primary metabolism

  9. Seed Hydropriming and Smoke Water Significantly Improve Low-Temperature Germination of Lupinus angustifolius L.

    Directory of Open Access Journals (Sweden)

    Agnieszka Płażek

    2018-03-01

    Full Text Available Seed imbibition under cold temperature is dangerous when dry seeds have relatively low water content. The aim of this study was to investigate germination of 20 lines/cultivars of narrow-leaf lupine at 7 °C (cold and 13 °C (control under the influence of smoke water and following seed hydropriming for 3 h at 20 °C. The efficacy of individual treatments was examined with regard to seed protection during low-temperature germination. Based on seed germination, vigour at cold was evaluated four days after sowing by means of hypocotyl length, the studied lines/cultivars were divided into three groups with low, high and very high germination rates. Germination vigour correlated with cell membrane permeability, dehydrogenase activity and abscisic acid (ABA content and was analysed in the seeds one day after sowing. Gibberellin content did not correlate with germination vigour. The seeds of weakly germinating lines/cultivars had the highest cell permeability and ABA content as well as the lowest amylolytic activity at both studied temperatures. Additionally, the vigour of weakly germinating seeds at 7 °C correlated with dehydrogenase activity. Three-hour hydropriming was the most effective for seed germination under cold due to reduced cell membrane permeability and ABA level. Stimulating effects of smoke water on germination under cold could be explained by enhanced dehydrogenase activity.

  10. Quality control of system of imaging for rectal ultrasound for implants seed prostate low rate

    International Nuclear Information System (INIS)

    Luquero Llopis, N.; Ferrer Gracia, C.; Huertas Martinez, C.; Huerga Cabrerizo, C.; Corredoira Silva, E.; Serrada Hierro, A.

    2013-01-01

    In this work, the objective is the evaluation of the image system used in implants of prostate of low rate held at our hospital, for maximum control on the placement of the seeds in the patient and therefore carried out dosimetry. (Author)

  11. Computer image analysis of seed shape and seed color for flax cultivar description

    Czech Academy of Sciences Publication Activity Database

    Wiesnerová, Dana; Wiesner, Ivo

    2008-01-01

    Roč. 61, č. 2 (2008), s. 126-135 ISSN 0168-1699 R&D Projects: GA ČR GA521/03/0019 Institutional research plan: CEZ:AV0Z50510513 Keywords : image analysis * cultivar description * flax Subject RIV: EA - Cell Biology Impact factor: 1.273, year: 2008

  12. Identification of {sup 192}Ir seeds in localization images using a novel statistical pattern recognition approach and a priori information

    Energy Technology Data Exchange (ETDEWEB)

    Bird, William F; Chaney, Edward L; Coggins, James M

    1995-07-01

    Purpose / Objective: Manual labeling of individual {sup 192}Ir seeds in localization images for dosimetry of multi-strand low-dose-rate (LDR) implants is labor intensive, tedious and prone to error. The objective of this investigation is to develop computer-based methods that analyze digitized localization images, improve dosimetric efficiency, and reduce labeling errors. Materials and Methods: {sup 192}Ir localization films were digitized with a scanned-laser system and analyzed using Multiscale, Geometric, Statistical Pattern Recognition (MGSPR), a technique that recognizes and classifies pixels in gray-scale images based on their surrounding, neighborhood geometry. To 'teach' MGSPR how to recognize specific objects, a Gaussian-based mathematical filter set is applied to training images containing user-labeled examples of the desired objects. The filters capture a broad range of descriptive geometric information at multiple spatial scales. Principled mathematical analysis is used to determine the linear combination of filters from a large base set that yields the best discrimination between object types. Thus the sensitivity of the filters can be 'tuned' to detect specific objects such as{sup 192} Ir seeds. For a given pixel, the output of the filter is a multi-component feature vector that uniquely describes the pixel's geometric characteristics. Pixels with similar geometric attributes have feature vectors that naturally 'cluster', or group, in the multidimensional space called 'feature space'. After statistically quantifying the training-set clusters in feature space, pixels found in new images are automatically labeled by correlation with the nearest cluster, e.g., the cluster representing {sup 192}Ir seeds. One of the greatest challenges in statistical pattern recognition is to determine which filters result in the best labeling. Good discrimination is achieved when clusters are compact and well isolated from one another in feature space. The filters used in

  13. Identification of 192Ir seeds in localization images using a novel statistical pattern recognition approach and a priori information

    International Nuclear Information System (INIS)

    Bird, William F.; Chaney, Edward L.; Coggins, James M.

    1995-01-01

    Purpose / Objective: Manual labeling of individual 192 Ir seeds in localization images for dosimetry of multi-strand low-dose-rate (LDR) implants is labor intensive, tedious and prone to error. The objective of this investigation is to develop computer-based methods that analyze digitized localization images, improve dosimetric efficiency, and reduce labeling errors. Materials and Methods: 192 Ir localization films were digitized with a scanned-laser system and analyzed using Multiscale, Geometric, Statistical Pattern Recognition (MGSPR), a technique that recognizes and classifies pixels in gray-scale images based on their surrounding, neighborhood geometry. To 'teach' MGSPR how to recognize specific objects, a Gaussian-based mathematical filter set is applied to training images containing user-labeled examples of the desired objects. The filters capture a broad range of descriptive geometric information at multiple spatial scales. Principled mathematical analysis is used to determine the linear combination of filters from a large base set that yields the best discrimination between object types. Thus the sensitivity of the filters can be 'tuned' to detect specific objects such as 192 Ir seeds. For a given pixel, the output of the filter is a multi-component feature vector that uniquely describes the pixel's geometric characteristics. Pixels with similar geometric attributes have feature vectors that naturally 'cluster', or group, in the multidimensional space called 'feature space'. After statistically quantifying the training-set clusters in feature space, pixels found in new images are automatically labeled by correlation with the nearest cluster, e.g., the cluster representing 192 Ir seeds. One of the greatest challenges in statistical pattern recognition is to determine which filters result in the best labeling. Good discrimination is achieved when clusters are compact and well isolated from one another in feature space. The filters used in this study are

  14. Influence of vineyard peach selections on vigour and initial yield in peach and nectarine

    Directory of Open Access Journals (Sweden)

    Zec Gordan

    2013-01-01

    Full Text Available Seedlings (progenies of eight selected vineyard peach genotypes obtained by self and open pollination were examined in this study during three consecutive years. Two progenies from unselected vineyard peach genotypes with different geographic origin were used as a standard. Peach cv. Autumn Glo and nectarine cv. Stark Red Gold were grafted on F1 generation of eight vineyard peach selections and two standard progenies. Analysis of variance indicated statistically significant differences in vigour and fruit productivity between fruit trees grafted on different progenies of vineyard peach selections. A high correlation was found between rootstock vigour and fruit production of grafted cultivars. In addition, the principal component analysis made it possible to establish similar groups of rootstocks, depending on its influence to vigour, productivity and indexes of efficiency of grafted peach and nectarine cultivar. The most promising rootstocks for those two cultivars were PSK and 7S because grafted AG and SRG have high fruit weight, initial yields and very satisfactory rootstock, scion and canopy efficiency. [Projekat Ministarstva nauke Republike Srbije, br. TR 31063

  15. Physiological changes and sHSPs genes relative transcription in relation to the acquisition of seed germination during maturation of hybrid rice seed.

    Science.gov (United States)

    Zhu, Li-Wei; Cao, Dong-Dong; Hu, Qi-Juan; Guan, Ya-Jing; Hu, Wei-Min; Nawaz, Aamir; Hu, Jin

    2016-03-30

    During the production of early hybrid rice seed, the seeds dehydrated slowly and retained high moisture levels when rainy weather lasted for a couple of days, and the rice seeds easily occurred pre-harvest sprouting (PHS) along with high temperature. Therefore it is necessary to harvest the seeds before the PHS occurred. The seeds of hybrid rice (Oryza sativa L. subsp. indica) cv. Qianyou No1 that harvests from 19 to 28 days after pollination (DAP) all had high seed vigour. The seed moisture content at 10 DAP was 36.1%, and declined to 28.6% at 19 DAP; the contents of soluble sugar and total starch increased significantly with the development of seeds. The soluble protein content, the level of abscisic acid (ABA) and gibberellin (GA3 ), and ascorbate peroxidase (APX) activity continued to decrease from 10 DAP to 19 DAP. The seeds at 19 DAP had the highest peroxidase (POD) activity and lowest catalase (CAT) activity while the superoxide dismutase (SOD) activity had no significant difference among the different developing periods. The relative expressions of genes 64S Hsp18.0 and Os03g0267200 transcripts increased significantly from 10 to 19 DAP, and then decreased. However, no significant change was recorded in soluble protein, sugar and GA3 after 16 DAP, and they all significantly correlated with seed viability and vigour during the process of seed maturity. The seeds of hybrid rice Qianyou No1 had a higher viability and vigour when harvested from 19 DAP to 28 DAP, the transcription levels of 64S Hsp18.0 and Os03g0267200 increased significantly from 10 DAP to 19 DAP and the highest value was recorded at 19 DAP. The seeds could be harvested as early as 19 DAP without negative influence on seed vigour and viability. © 2015 Society of Chemical Industry.

  16. Alleviate Seed Ageing Effects in Silybum marianum by Application of Hormone Seed Priming

    Directory of Open Access Journals (Sweden)

    Seyed Ata SIADAT

    2015-09-01

    Full Text Available Most of the medicinal plants have seed dormancy or poor seed germination. This is due to their natural compounds or their morphological structure. Existence of such mechanisms makes the seeds able to endure harsh environments, stressful conditions or natural ageing. Different seed enhancement treatments were proposed in order to improve seed germination. In this study, it was examined Silybum marianum seed longevity (0, 48 and 72 hour of ageing and the response to seed priming when using different concentrations of cytokinin (50, 200, 350 and 500 ppm and different duration of treatment (8, 12 and 24 hour. Results revealed that ageing could be successfully alleviated using seed priming. Seed ageing significantly affected seed germination and vigour. Application of cytokinin considerably improved seed vigour in aged and non aged seeds. The most effective seed priming treatment was with 500 ppm cytokine, with the duration of 24 hours. In conclusion, it can be recommend the used of plant growth regulators like cytokine, as a good priming agent, to recover losses of seed quality and improve germination characteristics.

  17. Experimental evaluation of an online gamma-camera imaging of permanent seed implantation (OGIPSI) prototype for partial breast irradiation

    International Nuclear Information System (INIS)

    Ravi, Ananth; Caldwell, Curtis B.; Pignol, Jean-Philippe

    2008-01-01

    Previously, our team used Monte Carlo simulation to demonstrate that a gamma camera could potentially be used as an online image guidance device to visualize seeds during permanent breast seed implant procedures. This could allow for intraoperative correction if seeds have been misplaced. The objective of this study is to describe an experimental evaluation of an online gamma-camera imaging of permanent seed implantation (OGIPSI) prototype. The OGIPSI device is intended to be able to detect a seed misplacement of 5 mm or more within an imaging time of 2 min or less. The device was constructed by fitting a custom built brass collimator (16 mm height, 0.65 mm hole pitch, 0.15 mm septal thickness) on a 64 pixel linear array CZT detector (eValuator-2000, eV Products, Saxonburg, PA). Two-dimensional projection images of seed distributions were acquired by the use of a digitally controlled translation stage. Spatial resolution and noise characteristics of the detector were measured. The ability and time needed for the OGIPSI device to image the seeds and to detect cold spots was tested using an anthropomorphic breast phantom. Mimicking a real treatment plan, a total of 52 103 Pd seeds of 65.8 MBq each were placed on three different layers at appropriate depths within the phantom. The seeds were reliably detected within 30 s with a median error in localization of 1 mm. In conclusion, an OGIPSI device can potentially be used for image guidance of permanent brachytherapy applications in the breast and, possibly, other sites

  18. Osmopriming on Sesbania virgata (CAV. PERS (Fabaceae seeds

    Directory of Open Access Journals (Sweden)

    Tathiana Elisa Masetto

    2013-12-01

    Full Text Available This study evaluated the effect of the osmopriming on germination and vigour of Sesbania virgata seeds. Seeds were chemically scarified in concentrated sulphuric acid for 40 minutes and put to germinate either directly or after being submitted to osmopriming, drying and accelerated aging. Osmopriming was carried out with polyethylene glycol solutions (PEG 8000 at the following osmotic potentials-0.2; -0.4; -0.6 and -0.8 MPa for 12, 24 and 48 hours. After osmopriming, seeds were dried in silica gel until the initial moisture content was reached, and then submitted to the accelerated aging (48 h/100% RH. The effects of osmopriming and accelerated aging were evaluated through germination test, first counting germination and germination speed index. The osmopriming, followed or not by accelerated aging, positively influenced germination and vigour of Sesbania virgata seeds.

  19. Non-Destructive Quality Evaluation of Pepper (Capsicum annuum L. Seeds Using LED-Induced Hyperspectral Reflectance Imaging

    Directory of Open Access Journals (Sweden)

    Changyeun Mo

    2014-04-01

    Full Text Available In this study, we developed a viability evaluation method for pepper (Capsicum annuum L. seeds based on hyperspectral reflectance imaging. The reflectance spectra of pepper seeds in the 400–700 nm range are collected from hyperspectral reflectance images obtained using blue, green, and red LED illumination. A partial least squares–discriminant analysis (PLS-DA model is developed to classify viable and non-viable seeds. Four spectral ranges generated with four types of LEDs (blue, green, red, and RGB, which were pretreated using various methods, are investigated to develop the classification models. The optimal PLS-DA model based on the standard normal variate for RGB LED illumination (400–700 nm yields discrimination accuracies of 96.7% and 99.4% for viable seeds and nonviable seeds, respectively. The use of images based on the PLS-DA model with the first-order derivative of a 31.5-nm gap for red LED illumination (600–700 nm yields 100% discrimination accuracy for both viable and nonviable seeds. The results indicate that a hyperspectral imaging technique based on LED light can be potentially applied to high-quality pepper seed sorting.

  20. Use of partial least squares discriminant analysis on visible-near infrared multispectral image data to examine germination ability and germ length in spinach seeds

    DEFF Research Database (Denmark)

    Shetty, Nisha; Olesen, Merete Halkjær; Gislum, René

    2012-01-01

    Because of the difficulties in obtaining homogenous germination of spinach seeds for baby leaf production, the possibility of using partial least squares discriminant analysis (PLS-DA) on features extracted from multispectral images of spinach seeds was investigated. The objective has been...... to discriminate between different seed sizes, as well as to predict germination ability and germ length. Images of 300 seeds including small, medium, and large seeds were taken, and the seeds were examined for germination ability and germ length. PLS-DA loadings plots were used to reduce the multidimensional...

  1. Functionalised alginate flow seeding microparticles for use in Particle Image Velocimetry (PIV).

    Science.gov (United States)

    Varela, Sylvana; Balagué, Isaac; Sancho, Irene; Ertürk, Nihal; Ferrando, Montserrat; Vernet, Anton

    2016-01-01

    Alginate microparticles as flow seeding fulfil all the requirements that are recommended for the velocity measurements in Particle Image Velocimetry (PIV). These spherical microparticles offer the advantage of being environmentally friendly, having excellent seeding properties and they can be produced via a very simple process. In the present study, the performances of alginate microparticles functionalised with a fluorescent dye, Rhodamine B (RhB), for PIV have been studied. The efficacy of fluorescence is appreciated in a number of PIV applications since it can boost the signal-to-noise ratio. Alginate microparticles functionalised with RhB have high emission efficiency, desirable match with fluid density and controlled size. The study of the particles behaviour in strong acid and basic solutions and ammonia is also included. This type of particles can be used for measurements with PIV and Planar Laser Induced Fluorescence (PLIF) simultaneously, including acid-base reactions.

  2. Locations of radical species in black pepper seeds investigated by CW EPR and 9GHz EPR imaging.

    Science.gov (United States)

    Nakagawa, Kouichi; Epel, Boris

    2014-10-15

    In this study, noninvasive 9GHz electron paramagnetic resonance (EPR)-imaging and continuous wave (CW) EPR were used to investigate the locations of paramagnetic species in black pepper seeds without further irradiation. First, lithium phthalocyanine (LiPC) phantom was used to examine 9GHz EPR imaging capabilities. The 9GHz EPR-imager easily resolved the LiPC samples at a distance of ∼2mm. Then, commercially available black pepper seeds were measured. We observed signatures from three different radical species, which were assigned to stable organic radicals, Fe(3+), and Mn(2+) complexes. In addition, no EPR spectral change in the seed was observed after it was submerged in distilled H2O for 1h. The EPR and spectral-spatial EPR imaging results suggested that the three paramagnetic species were mostly located at the seed surface. Fewer radicals were found inside the seed. We demonstrated that the CW EPR and 9GHz EPR imaging were useful for the determination of the spatial distribution of paramagnetic species in various seeds. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Optimum harvest maturity for Leymus chinensis seed

    Directory of Open Access Journals (Sweden)

    Jixiang Lin

    2016-06-01

    Full Text Available Timely harvest is critical to achieve maximum seed viability and vigour in agricultural production. However, little information exists concerning how to reap the best quality seeds of Leymus chinensis, which is the dominant and most promising grass species in the Songnen Grassland of Northern China. The objective of this study was to investigate and evaluate possible quality indices of the seeds at different days after peak anthesis. Seed quality at different development stages was assessed by the colours of the seed and lemmas, seed weight, moisture content, electrical conductivity of seed leachate and germination indices. Two consecutive years of experimental results showed that the maximum seed quality was recorded at 39 days after peak anthesis. At this date, the colours of the seed and lemmas reached heavy brown and yellow, respectively. The seed weight was highest and the moisture content and the electrical conductivity of seed leachate were lowest. In addition, the seed also reached its maximum germination percentage and energy at this stage, determined using a standard germination test (SGT and accelerated ageing test (AAT. Thus, Leymus chinensis can be harvested at 39 days after peak anthesis based on the changes in parameters. Colour identification can be used as an additional indicator to provide a more rapid and reliable measure of optimum seed maturity; approximately 10 days after the colour of the lemmas reached yellow and the colour of the seed reached heavy brown, the seed of this species was suitable for harvest.

  4. Automatic detection of the macula in retinal fundus images using seeded mode tracking approach.

    Science.gov (United States)

    Wong, Damon W K; Liu, Jiang; Tan, Ngan-Meng; Yin, Fengshou; Cheng, Xiangang; Cheng, Ching-Yu; Cheung, Gemmy C M; Wong, Tien Yin

    2012-01-01

    The macula is the part of the eye responsible for central high acuity vision. Detection of the macula is an important task in retinal image processing as a landmark for subsequent disease assessment, such as for age-related macula degeneration. In this paper, we have presented an approach to automatically determine the macula centre in retinal fundus images. First contextual information on the image is combined with a statistical model to obtain an approximate macula region of interest localization. Subsequently, we propose the use of a seeded mode tracking technique to locate the macula centre. The proposed approach is tested on a large dataset composed of 482 normal images and 162 glaucoma images from the ORIGA database and an additional 96 AMD images. The results show a ROI detection of 97.5%, and 90.5% correct detection of the macula within 1/3DD from a manual reference, which outperforms other current methods. The results are promising for the use of the proposed approach to locate the macula for the detection of macula diseases from retinal images.

  5. Application of image processing to calculate the number of fish seeds using raspberry-pi

    Science.gov (United States)

    Rahmadiansah, A.; Kusumawardhani, A.; Duanto, F. N.; Qoonita, F.

    2018-03-01

    Many fish cultivator in Indonesia who suffered losses due to the sale and purchase of fish seeds did not match the agreed amount. The loss is due to the calculation of fish seed still using manual method. To overcome these problems, then in this study designed fish counting system automatically and real-time fish using the image processing based on Raspberry Pi. Used image processing because it can calculate moving objects and eliminate noise. Image processing method used to calculate moving object is virtual loop detector or virtual detector method and the approach used is “double difference image”. The “double difference” approach uses information from the previous frame and the next frame to estimate the shape and position of the object. Using these methods and approaches, the results obtained were quite good with an average error of 1.0% for 300 individuals in a test with a virtual detector width of 96 pixels and a slope of 1 degree test plane.

  6. Burnout, vigour, big five personality traits and social support in a sample of police officers

    Directory of Open Access Journals (Sweden)

    Gerrit J. Louw

    2014-08-01

    Research purpose: The purpose of the study was to obtain an understanding of a police officer’s need to reduce the effects of prolonged stressors and to utilise personal and social sources to mitigate the effects of negative stressors. Motivation for the study: Burnout causes negative health consequences for police officers. Those who are affected reflect withdrawal behaviour through operational disruption or lower productivity in the work context. Research design, approach and method: The research has a non-experimental design in the quantitative tradition with a cross-sectional data-collection method. The availability sample (N = 505 included participants from different demographic backgrounds. A survey was used to measure all constructs at a single point in time. Main findings: Neuroticism and a lack of family support predicted burnout whilst conscientiousness and emotional stability predicted vigour. This was followed by significant other, which predicted physical strength, friend support which predicted emotional energy and family support which predicted cognitive liveliness. Practical/managerial implications: The study provides markers for selection purposes and calls on police management to be aware of the value of social support in the prevention of burnout whilst encouraging supervisor support in operational designs. Contribution/value-add: The study adds to the body of knowledge on the role of personality and social support in burnout and vigour in police officers.

  7. SU-D-201-06: Random Walk Algorithm Seed Localization Parameters in Lung Positron Emission Tomography (PET) Images

    Energy Technology Data Exchange (ETDEWEB)

    Soufi, M [Shahid Beheshti University, Tehran, Tehran (Iran, Islamic Republic of); Asl, A Kamali [Shahid Beheshti University, Tehran, Iran., Tehran, Tehran (Iran, Islamic Republic of); Geramifar, P [Shariati Hospital, Tehran, Iran., Tehran, Tehran (Iran, Islamic Republic of)

    2015-06-15

    Purpose: The objective of this study was to find the best seed localization parameters in random walk algorithm application to lung tumor delineation in Positron Emission Tomography (PET) images. Methods: PET images suffer from statistical noise and therefore tumor delineation in these images is a challenging task. Random walk algorithm, a graph based image segmentation technique, has reliable image noise robustness. Also its fast computation and fast editing characteristics make it powerful for clinical purposes. We implemented the random walk algorithm using MATLAB codes. The validation and verification of the algorithm have been done by 4D-NCAT phantom with spherical lung lesions in different diameters from 20 to 90 mm (with incremental steps of 10 mm) and different tumor to background ratios of 4:1 and 8:1. STIR (Software for Tomographic Image Reconstruction) has been applied to reconstruct the phantom PET images with different pixel sizes of 2×2×2 and 4×4×4 mm{sup 3}. For seed localization, we selected pixels with different maximum Standardized Uptake Value (SUVmax) percentages, at least (70%, 80%, 90% and 100%) SUVmax for foreground seeds and up to (20% to 55%, 5% increment) SUVmax for background seeds. Also, for investigation of algorithm performance on clinical data, 19 patients with lung tumor were studied. The resulted contours from algorithm have been compared with nuclear medicine expert manual contouring as ground truth. Results: Phantom and clinical lesion segmentation have shown that the best segmentation results obtained by selecting the pixels with at least 70% SUVmax as foreground seeds and pixels up to 30% SUVmax as background seeds respectively. The mean Dice Similarity Coefficient of 94% ± 5% (83% ± 6%) and mean Hausdorff Distance of 1 (2) pixels have been obtained for phantom (clinical) study. Conclusion: The accurate results of random walk algorithm in PET image segmentation assure its application for radiation treatment planning and

  8. SU-D-201-06: Random Walk Algorithm Seed Localization Parameters in Lung Positron Emission Tomography (PET) Images

    International Nuclear Information System (INIS)

    Soufi, M; Asl, A Kamali; Geramifar, P

    2015-01-01

    Purpose: The objective of this study was to find the best seed localization parameters in random walk algorithm application to lung tumor delineation in Positron Emission Tomography (PET) images. Methods: PET images suffer from statistical noise and therefore tumor delineation in these images is a challenging task. Random walk algorithm, a graph based image segmentation technique, has reliable image noise robustness. Also its fast computation and fast editing characteristics make it powerful for clinical purposes. We implemented the random walk algorithm using MATLAB codes. The validation and verification of the algorithm have been done by 4D-NCAT phantom with spherical lung lesions in different diameters from 20 to 90 mm (with incremental steps of 10 mm) and different tumor to background ratios of 4:1 and 8:1. STIR (Software for Tomographic Image Reconstruction) has been applied to reconstruct the phantom PET images with different pixel sizes of 2×2×2 and 4×4×4 mm 3 . For seed localization, we selected pixels with different maximum Standardized Uptake Value (SUVmax) percentages, at least (70%, 80%, 90% and 100%) SUVmax for foreground seeds and up to (20% to 55%, 5% increment) SUVmax for background seeds. Also, for investigation of algorithm performance on clinical data, 19 patients with lung tumor were studied. The resulted contours from algorithm have been compared with nuclear medicine expert manual contouring as ground truth. Results: Phantom and clinical lesion segmentation have shown that the best segmentation results obtained by selecting the pixels with at least 70% SUVmax as foreground seeds and pixels up to 30% SUVmax as background seeds respectively. The mean Dice Similarity Coefficient of 94% ± 5% (83% ± 6%) and mean Hausdorff Distance of 1 (2) pixels have been obtained for phantom (clinical) study. Conclusion: The accurate results of random walk algorithm in PET image segmentation assure its application for radiation treatment planning and

  9. SU-E-J-233: Effect of Brachytherapy Seed Artifacts in T2 and Proton Density Maps in MR Images

    Energy Technology Data Exchange (ETDEWEB)

    Mashouf, S [Sunnybrook Odette Cancer Centre, Toronto, Ontario (Canada); University of Toronto, Dept of Radiation Oncology, Toronto, Ontario (Canada); Fatemi-Ardekani, A [Sunnybrook Odette Cancer Centre, Toronto, Ontario (Canada); Sunnybrook Research Institute, Toronto, Ontario (Canada); Song, W [Sunnybrook Odette Cancer Centre, Toronto, Ontario (Canada); University of Toronto, Dept of Radiation Oncology, Toronto, Ontario (Canada); Sunnybrook Research Institute, Toronto, Ontario (Canada)

    2015-06-15

    Purpose: This study aims at investigating the influence of brachytherapy seeds on T2 and proton density (PD) maps generated from MR images. Proton density maps can be used to extract water content. Since dose absorbed in tissue surrounding low energy brachytherapy seeds are highly influenced by tissue composition, knowing the water content is a first step towards implementing a heterogeneity correction algorithm using MR images. Methods: An LDR brachytherapy (IsoAid Advantage Pd-103) seed was placed in the middle of an agar-based gel phantom and imaged using a 3T Philips MR scanner with a 168-channel head coil. A multiple echo sequence with TE=20, 40, 60, 80, 100 (ms) with large repetition time (TR=6259ms) was used to extract T2 and PD maps. Results: Seed artifacts were considerably reduced on T2 maps compared to PD maps. The variation of PD around the mean was obtained as −97% to 125% (±1%) while for T2 it was recorded as −71% to 24% (±1%). Conclusion: PD maps which are required for heterogeneity corrections are susceptible to artifacts from seeds. Seed artifacts on T2 maps, however, are significantly reduced due to not being sensitive to B0 field variation.

  10. Non-destructive evaluation of bacteria-infected watermelon seeds using Vis/NIR hyperspectral imaging

    Science.gov (United States)

    It is needed to minimize the economic loss by sorting infected seeds from healthy seeds before seeding. However, current methods of detecting infection seeds such as seedling grow-out, enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), and real-time polymerase chain reaction...

  11. Burnout, vigour, big five personality traits and social support in a sample of police officers

    Directory of Open Access Journals (Sweden)

    Gerrit J. Louw

    2014-08-01

    Full Text Available Orientation: Burnout occurs as a result of prolonged job stress, which is a phenomenon prevalent amongst police officers in South Africa. Whilst some suffer from burnout, others elude the pathological effects of burnout and execute their duties vigorously under the same circumstances. Research purpose: The purpose of the study was to obtain an understanding of a police officer’s need to reduce the effects of prolonged stressors and to utilise personal and social sources to mitigate the effects of negative stressors. Motivation for the study: Burnout causes negative health consequences for police officers. Those who are affected reflect withdrawal behaviour through operational disruption or lower productivity in the work context. Research design, approach and method: The research has a non-experimental design in the quantitative tradition with a cross-sectional data-collection method. The availability sample (N = 505 included participants from different demographic backgrounds. A survey was used to measure all constructs at a single point in time. Main findings: Neuroticism and a lack of family support predicted burnout whilst conscientiousness and emotional stability predicted vigour. This was followed by significant other, which predicted physical strength, friend support which predicted emotional energy and family support which predicted cognitive liveliness. Practical/managerial implications: The study provides markers for selection purposes and calls on police management to be aware of the value of social support in the prevention of burnout whilst encouraging supervisor support in operational designs. Contribution/value-add: The study adds to the body of knowledge on the role of personality and social support in burnout and vigour in police officers.

  12. Impact of selenium enrichment on seed potato tubers

    Directory of Open Access Journals (Sweden)

    M. TURAKAINEN

    2008-12-01

    Full Text Available The aim of this study was to investigate the effect of Se enrichment on the growth of sprouts and growth vigour of seed potatoes (Solanum tuberosum L. stored for 2 to 8 months. Our results showed that Se did not affect the duration of dormancy. At the high addition levels (0.075 and 0.9 mg kg-1 quartz sand, Se had some positive effects on the growth of sprouts. The peak sprouting capacity was reached after 8 months of storage. The highest Se enrichment of tubers had some positive effect on the free putrescine content in sprouts. However, the better growth of sprouts was not consistent with the growth vigour of the seed tubers and yield produced. Selenium had no significant effect on the malondialdehyde (MDA or on the concentration of soluble sugars and starch. No significant effect of added Se on the early growth, stem and tuber numbers and yield parameters was observed. Irrespective of the level of Se added, the highest yield was harvested from plants produced with seed tubers stored for 6 months. Our results indicate that Se had some positive effects on the growth of sprouts, but it had no consistent effect on the growth vigour of seed tubers.;

  13. Improvement of lettuce seedling vigour after far red irradiation of aged achenes

    International Nuclear Information System (INIS)

    Górski, T.

    1993-01-01

    Far red treatment of aged achenes of lettuce (Lactuca sativa L.) during 4 days after inhibition usually reduced the percentage of seedlings with developmental abnormities. This effect was retained also in redried seeds. The field test yielded a greater number of plants emerging from treated than from control seeds. The results are similar to seed invigoration by other factors inhibiting an immediate germination of imbibed seeds

  14. Some agricultural aspects of seed longevity (literature review

    Directory of Open Access Journals (Sweden)

    Roman Hołubowicz

    2013-12-01

    Full Text Available There has been a vast and numerous literature concerning seed longevity. Most of these works however has focused solely on theoretical and biological aspects of this problem. Some works although deal with practical problems of seed storage. vigour or deterioration. have had relatively little connection with agricultural crops. practical farming or gardening. Therefore, there has been a need to look at this problem from the seednian's and farmer's point of view. The paper comments on how long seeds of agricultural crops species can keep their longevity, how long it is economically reasonable to store them. the effect of the seeds chemical composition on their longevity, how seeds storage conditions can be modified in order to eventually improve their longevity and environment circumstances influence on the final seed longevity. The paper contains a synthetic summary of expected seed longevity of the most important agricultural species and many examples of long longevity of agricultural species.

  15. Development of Auto-Seeding System Using Image Processing Technology in the Sapphire Crystal Growth Process via the Kyropoulos Method

    Directory of Open Access Journals (Sweden)

    Churl Min Kim

    2017-04-01

    Full Text Available The Kyropoulos (Ky and Czochralski (Cz methods of crystal growth are used for large-diameter single crystals. The seeding process in these methods must induce initial crystallization by initiating contact between the seed crystals and the surface of the melted material. In the Ky and Cz methods, the seeding process lays the foundation for ingot growth during the entire growth process. When any defect occurs in this process, it is likely to spread to the entire ingot. In this paper, a vision system was constructed for auto seeding and for observing the surface of the melt in the Ky method. An algorithm was developed to detect the time when the internal convection of the melt is stabilized by observing the shape of the spoke pattern on the melt material surface. Then, the vision system and algorithm were applied to the growth furnace, and the possibility of process automation was examined for sapphire growth. To confirm that the convection of the melt was stabilized, the position of the island (i.e., the center of a spoke pattern was detected using the vision system and image processing. When the observed coordinates for the center of the island were compared with the coordinates detected from the image processing algorithm, there was an average error of 1.87 mm (based on an image with 1024 × 768 pixels.

  16. Germination of red alder (Alnus rubra) seed from several locations in its natural range

    Energy Technology Data Exchange (ETDEWEB)

    Elliott, D.M.; Taylor, I.E.P.

    1981-01-01

    Tests were carried out on stratified, pre-soaked or untreated seeds. Germination of all provenances and treatments was more than 90%, except for one provenance showing physiological dormancy in which germination was low, but increased significantly with stratification and presoaking; dormancy could be broken completely by chilling at 0 degrees C for 5 days. Significant interactions were found between provenances and treatments for both germination % and seed vigour (as measured by germination value).

  17. Study on Development of Non-Destructive Measurement Technique for Viability of Lettuce Seed (Lactuca sativa L) Using Hyperspectral Reflectance Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Ahn, Chi Kook; Cho, Byoung Kwan [College of Agriculture and Life Science, Chungnam National University, Daejeon (Korea, Republic of); Mo, Chang Yeon [National Acadamy of Agricultural Science, Daejeon (Korea, Republic of); Kim, Moon S. [Environmental Microbial and Food Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Washington (United States)

    2012-10-15

    In this study, the feasibility of hyperspectral reflectance imaging technique was investigated for the discrimination of viable and non-viable lettuce seeds. The spectral data of hyperspectral reflectance images with the spectral range between 750 nm and 1000 nm were used to develop PLS-DA model for the classification of viable and non-viable lettuce seeds. The discrimination accuracy of the calibration set was 81.6% and that of the test set was 81.2%. The image analysis method was developed to construct the discriminant images of non-viable seeds with the developed PLS-DA model. The discrimination accuracy obtained from the resultant image were 91%, which showed the feasibility of hyperspectral reflectance imaging technique for the mass discrimination of non-viable lettuce seeds from viable ones.

  18. Study on Development of Non-Destructive Measurement Technique for Viability of Lettuce Seed (Lactuca sativa L) Using Hyperspectral Reflectance Imaging

    International Nuclear Information System (INIS)

    Ahn, Chi Kook; Cho, Byoung Kwan; Mo, Chang Yeon; Kim, Moon S.

    2012-01-01

    In this study, the feasibility of hyperspectral reflectance imaging technique was investigated for the discrimination of viable and non-viable lettuce seeds. The spectral data of hyperspectral reflectance images with the spectral range between 750 nm and 1000 nm were used to develop PLS-DA model for the classification of viable and non-viable lettuce seeds. The discrimination accuracy of the calibration set was 81.6% and that of the test set was 81.2%. The image analysis method was developed to construct the discriminant images of non-viable seeds with the developed PLS-DA model. The discrimination accuracy obtained from the resultant image were 91%, which showed the feasibility of hyperspectral reflectance imaging technique for the mass discrimination of non-viable lettuce seeds from viable ones.

  19. NEW TECHNIQUES FOR HIGH-CONTRAST IMAGING WITH ADI: THE ACORNS-ADI SEEDS DATA REDUCTION PIPELINE

    International Nuclear Information System (INIS)

    Brandt, Timothy D.; Turner, Edwin L.; McElwain, Michael W.; Grady, C. A.; Abe, L.; Brandner, W.; Feldt, M.; Henning, T.; Carson, J.; Egner, S.; Golota, T.; Guyon, O.; Hayano, Y.; Hayashi, S.; Ishii, M.; Goto, M.; Hashimoto, J.; Hayashi, M.; Iye, M.; Hodapp, K. W.

    2013-01-01

    We describe Algorithms for Calibration, Optimized Registration, and Nulling the Star in Angular Differential Imaging (ACORNS-ADI), a new, parallelized software package to reduce high-contrast imaging data, and its application to data from the SEEDS survey. We implement several new algorithms, including a method to register saturated images, a trimmed mean for combining an image sequence that reduces noise by up to ∼20%, and a robust and computationally fast method to compute the sensitivity of a high-contrast observation everywhere on the field of view without introducing artificial sources. We also include a description of image processing steps to remove electronic artifacts specific to Hawaii2-RG detectors like the one used for SEEDS, and a detailed analysis of the Locally Optimized Combination of Images (LOCI) algorithm commonly used to reduce high-contrast imaging data. ACORNS-ADI is written in python. It is efficient and open-source, and includes several optional features which may improve performance on data from other instruments. ACORNS-ADI requires minimal modification to reduce data from instruments other than HiCIAO. It is freely available for download at www.github.com/t-brandt/acorns-adi under a Berkeley Software Distribution (BSD) license.

  20. Selective Seed Abortion Affects the Performance of the Offspring in Bauhinia ungulata

    Science.gov (United States)

    MENA-ALÍ, JORGE I.; ROCHA, OSCAR J.

    2005-01-01

    • Background and Aims Under the microgametophytic competition hypothesis, a non-random pattern of seed abortion is expected, in which only the most vigorous seeds reach maturity. In a previous study, it was found that Bauhinia ungulata (Fabaceae) exhibits a pattern of seed abortion dependent on the position of the ovule within the ovary; ovules located in the stylar half of the fruit, close to the point of entry of pollen tubes to the ovary, have a low probability of seed abortion, whereas ovules in the basal half of the fruit are aborted with a high probability. • Methods A series of experimental fruits was generated, in which ovules from either the stylar (treatments 1 and 2) or the basal (treatments 3 and 4) half of fruits were destroyed, to evaluate whether these patterns of selective seed abortion have an effect on the vigour of the offspring in B. ungulata. • Key Results Only 53 % of the seed from control fruits germinated. Seed set in fruits from treatments 1 and 2 showed a significantly lower (33–43 %) percentage of germination; the germination of seeds from fruits in treatments 3 and 4 (49–51 %) did not differ from control seeds. In addition, it was found that the differences in vigour of the offspring are not random with respect to the position of the ovule in the pod. • Conclusions The overall performance of the seeds correlated with their likelihood of maturation. Seeds located at the basal half of the treatment fruits showed lower values of vigour than seeds located on the stylar half. The differences were more marked for early measures of fitness. PMID:15749749

  1. THE SEEDS DIRECT IMAGING SURVEY FOR PLANETS AND SCATTERED DUST EMISSION IN DEBRIS DISK SYSTEMS

    Energy Technology Data Exchange (ETDEWEB)

    Janson, Markus; Brandt, Timothy D. [Department of Astrophysical Sciences, Princeton University, NJ 08544 (United States); Moro-Martin, Amaya [Department of Astrophysics, CAB (INTA-CSIC), Instituto Nacional de Tecnica Aerospacial, Torrejonde Ardoz, E-28850 Madrid (Spain); Usuda, Tomonori; Kudo, Tomoyuki; Egner, Sebastian [Subaru Telescope, 650 North Aohoku Place, Hilo, HI 96720 (United States); Thalmann, Christian [Astronomical Institute ' ' Anton Pannekoek' ' , University of Amsterdam, Science Park 904, 1098-XH Amsterdam (Netherlands); Carson, Joseph C. [Department of Physics and Astronomy, College of Charleston, 58 Coming Street, Charleston, SC 29424 (United States); Goto, Miwa [Universitaets-Sternwarte Muenchen, Ludwig-Maximilians-Universitaet, Scheinerstr. 1, D-81679 Munich (Germany); Currie, Thayne [Department of Astronomy and Astrophysics, University of Toronto, 50 St. George Street, M5S 3H4 Toronto, ON (Canada); McElwain, M. W. [Exoplanets and Stellar Astrophysics Laboratory, Code 667, Goddard Space Flight Center, Greenbelt, MD 2071 (United States); Itoh, Yoichi [Nishi-Harima Astronomical Observatory, Center for Astronomy, University of Hyogo, 407-2 Nishigaichi, Sayo, Hyogo 679-5313 (Japan); Fukagawa, Misato [Department of Earth and Space Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043 (Japan); Crepp, Justin [Department of Physics, University of Notre Dame, 225 Nieuwland Science Hall, Notre Dame, IN 46556 (United States); Kuzuhara, Masayuki; Hashimoto, Jun; Kusakabe, Nobuhiko [National Astronomical Observatory of Japan, 2-21-1 Osawa, Mitaka, Tokyo 181-8588 (Japan); Abe, Lyu [Laboratoire Lagrange, UMR7239, University of Nice-Sophia Antipolis, CNRS, Observatoire de la Cote d' Azur, F-06300 Nice (France); Brandner, Wolfgang; Feldt, Markus, E-mail: janson@astro.princeton.edu [Max Planck Institute for Astronomy, Koenigstuhl 17, D-69117 Heidelberg (Germany); and others

    2013-08-10

    Debris disks around young main-sequence stars often have gaps and cavities which for a long time have been interpreted as possibly being caused by planets. In recent years, several giant planet discoveries have been made in systems hosting disks of precisely this nature, further implying that interactions with planets could be a common cause of such disk structures. As part of the SEEDS high-contrast imaging survey, we are surveying a population of debris-disk-hosting stars with gaps and cavities implied by their spectral energy distributions, in order to attempt to spatially resolve the disk as well as to detect any planets that may be responsible for the disk structure. Here, we report on intermediate results from this survey. Five debris disks have been spatially resolved, and a number of faint point sources have been discovered, most of which have been tested for common proper motion, which in each case has excluded physical companionship with the target stars. From the detection limits of the 50 targets that have been observed, we find that {beta} Pic b-like planets ({approx}10 M{sub jup} planets around G-A-type stars) near the gap edges are less frequent than 15%-30%, implying that if giant planets are the dominant cause of these wide (27 AU on average) gaps, they are generally less massive than {beta} Pic b.

  2. Determination of Seed Soundness in Conifers Cryptomeria japonica and Chamaecyparis obtusa Using Narrow-Multiband Spectral Imaging in the Short-Wavelength Infrared Range

    Science.gov (United States)

    Matsuda, Osamu; Hara, Masashi; Tobita, Hiroyuki; Yazaki, Kenichi; Nakagawa, Toshinori; Shimizu, Kuniyoshi; Uemura, Akira; Utsugi, Hajime

    2015-01-01

    Regeneration of planted forests of Cryptomeria japonica (sugi) and Chamaecyparis obtuse (hinoki) is the pressing importance to the forest administration in Japan. Low seed germination rate of these species, however, has hampered low-cost production of their seedlings for reforestation. The primary cause of the low germinability has been attributed to highly frequent formation of anatomically unsound seeds, which are indistinguishable from sound germinable seeds by visible observation and other common criteria such as size and weight. To establish a method for sound seed selection in these species, hyperspectral imaging technique was used to identify a wavelength range where reflectance spectra differ clearly between sound and unsound seeds. In sound seeds of both species, reflectance in a narrow waveband centered at 1,730 nm, corresponding to a lipid absorption band in the short-wavelength infrared (SWIR) range, was greatly depressed relative to that in adjacent wavebands on either side. Such depression was absent or less prominent in unsound seeds. Based on these observations, a reflectance index SQI, abbreviated for seed quality index, was formulated using reflectance at three narrow SWIR wavebands so that it represents the extent of the depression. SQI calculated from seed area-averaged reflectance spectra and spatial distribution patterns of pixelwise SQI within each seed area were both proven as reliable criteria for sound seed selection. Enrichment of sound seeds was accompanied by an increase in germination rate of the seed lot. Thus, the methods described are readily applicable toward low-cost seedling production in combination with single seed sowing technology. PMID:26083366

  3. Effect of drought stress on growth, yield and seed quality of tomato (lycopersicon esculentum L.)

    International Nuclear Information System (INIS)

    Pervez, M.A.; Ayub, C.M.

    2009-01-01

    Plant growth is seriously affected by abiotic stresses such as drought, salinity or temperature. Drought is one of the most important limiting factors for agricultural crops and vegetable production in particular all around the world. Drought stress during vegetative or early reproductive growth usually reduces yield by reducing the number of seeds, seed size and seed quality. To assess the effect of drought stress on seed yield, seed quality and growth of tomato, the experiment was conducted in green house in plastic pots at Pen-y-Fridd field station, University of Wales, Bangor, U.K. during 2003-2004. Tomato cv. Moneymaker was used as a test crop. There were four treatments i.e. early stress (when first truss has set the fruits), middle stress (when fruits in first truss were fully matured and started changing their colour), late stress (when fruits on first truss were ripened fully), whereas in control no stress was imposed. Analysis of data regarding various attributes (fruit weight and shoot dry weight per plant, number of seeds per fruit, total number of seeds and seed weight per plant and vigour of seed) showed that drought stress had non-significant effect on vigour, quality and yield of tomato seed. Plant height, number of leaves and number of fruits per plant showed significant results toward drought stress signifying drought effects on growth of tomato. (author)

  4. WE-AB-BRA-11: Improved Imaging of Permanent Prostate Brachytherapy Seed Implants by Combining an Endorectal X-Ray Sensor with a CT Scanner

    International Nuclear Information System (INIS)

    Steiner, J; Matthews, K; Jia, G

    2016-01-01

    Purpose: To test feasibility of the use of a digital endorectal x-ray sensor for improved image resolution of permanent brachytherapy seed implants compared to conventional CT. Methods: Two phantoms simulating the male pelvic region were used to test the capabilities of a digital endorectal x-ray sensor for imaging permanent brachytherapy seed implants. Phantom 1 was constructed from acrylic plastic with cavities milled in the locations of the prostate and the rectum. The prostate cavity was filled a Styrofoam plug implanted with 10 training seeds. Phantom 2 was constructed from tissue-equivalent gelatins and contained a prostate phantom implanted with 18 strands of training seeds. For both phantoms, an intraoral digital dental x-ray sensor was placed in the rectum within 2 cm of the seed implants. Scout scans were taken of the phantoms over a limited arc angle using a CT scanner (80 kV, 120–200 mA). The dental sensor was removed from the phantoms and normal helical CT and scout (0 degree) scans using typical parameters for pelvic CT (120 kV, auto-mA) were collected. A shift-and add tomosynthesis algorithm was developed to localize seed plane location normal to detector face. Results: The endorectal sensor produced images with improved resolution compared to CT scans. Seed clusters and individual seed geometry were more discernable using the endorectal sensor. Seed 3D locations, including seeds that were not located in every projection image, were discernable using the shift and add algorithm. Conclusion: This work shows that digital endorectal x-ray sensors are a feasible method for improving imaging of permanent brachytherapy seed implants. Future work will consist of optimizing the tomosynthesis technique to produce higher resolution, lower dose images of 1) permanent brachytherapy seed implants for post-implant dosimetry and 2) fine anatomic details for imaging and managing prostatic disease compared to CT images. Funding: LSU Faculty Start-up Funding

  5. WE-AB-BRA-11: Improved Imaging of Permanent Prostate Brachytherapy Seed Implants by Combining an Endorectal X-Ray Sensor with a CT Scanner

    Energy Technology Data Exchange (ETDEWEB)

    Steiner, J; Matthews, K; Jia, G [Louisiana State University, Baton Rouge, LA (United States)

    2016-06-15

    Purpose: To test feasibility of the use of a digital endorectal x-ray sensor for improved image resolution of permanent brachytherapy seed implants compared to conventional CT. Methods: Two phantoms simulating the male pelvic region were used to test the capabilities of a digital endorectal x-ray sensor for imaging permanent brachytherapy seed implants. Phantom 1 was constructed from acrylic plastic with cavities milled in the locations of the prostate and the rectum. The prostate cavity was filled a Styrofoam plug implanted with 10 training seeds. Phantom 2 was constructed from tissue-equivalent gelatins and contained a prostate phantom implanted with 18 strands of training seeds. For both phantoms, an intraoral digital dental x-ray sensor was placed in the rectum within 2 cm of the seed implants. Scout scans were taken of the phantoms over a limited arc angle using a CT scanner (80 kV, 120–200 mA). The dental sensor was removed from the phantoms and normal helical CT and scout (0 degree) scans using typical parameters for pelvic CT (120 kV, auto-mA) were collected. A shift-and add tomosynthesis algorithm was developed to localize seed plane location normal to detector face. Results: The endorectal sensor produced images with improved resolution compared to CT scans. Seed clusters and individual seed geometry were more discernable using the endorectal sensor. Seed 3D locations, including seeds that were not located in every projection image, were discernable using the shift and add algorithm. Conclusion: This work shows that digital endorectal x-ray sensors are a feasible method for improving imaging of permanent brachytherapy seed implants. Future work will consist of optimizing the tomosynthesis technique to produce higher resolution, lower dose images of 1) permanent brachytherapy seed implants for post-implant dosimetry and 2) fine anatomic details for imaging and managing prostatic disease compared to CT images. Funding: LSU Faculty Start-up Funding

  6. Enhanced ULtrasound Visualization of Brachytherapy Seeds by a Novel Magnetically Induced Motion Image Method

    National Research Council Canada - National Science Library

    McAleavey, Stephen A

    2006-01-01

    ... to determine the sensitivity of torque to geometry. We have created a finite element model of a prototype coil configuration for seed vibration and demonstrated the ability of this coil configuration to steer the magnetic field in a manner suitable...

  7. Spectral CT with monochromatic imaging and metal artifacts reduction software for artifacts reduction of ¹²⁵I radioactive seeds in liver brachytherapy.

    Science.gov (United States)

    Yang, Qiuxia; Peng, Sheng; Wu, Jing; Ban, Xiaohua; He, Mingyan; Xie, Chuanmiao; Zhang, Rong

    2015-11-01

    To investigate the optimal monochromatic energy for artifacts reduction from (125)I seeds as well as image improvement in the vicinity of seeds on monochromatic images with and without metal artifacts reduction software (MARS) and to compare this with traditional 120-kVp images, so as to evaluate the application value of gemstone spectral imaging for reducing artifacts from (125)I seeds in liver brachytherapy. A total of 45 tumors from 25 patients treated with (125)I seed brachytherapy in the liver were enrolled in this study. Multiphasic spectral computed tomography (CT) scanning was performed for each patient. After a delay time of 15 s of portal vein phase, a traditional 120-kVp scan was performed, focusing on several planes of (125)I seeds only. The artifact index (AI) in the vicinity of seeds and the standard deviation (SD) of the CT density of region of interest in the outside liver parenchyma were calculated. Artifact appearance was evaluated and classified on reconstructed monochromatic S and 120-kVp images. Image quality in the vicinity of seeds of three data sets were evaluated using a 1-5 scale scoring method. The Friedman rank-sum test was used to estimate the scoring results of image quality. The greatest noise in monochromatic images was found at 40 keV (SD = 27.38, AI = 206.40). The optimal monochromatic energy was found at 75 keV, which provided almost the least image noise (SD = 10.01) and good performance in artifact reduction (AI = 102.73). Image noise and AI reduction at 75 keV was decreased by 63.44 and 50.23%, compared with at 40 keV. Near-field thick artifacts were obvious in all 45 lesions, in 120-kVp images, and 75-keV images, but basically reduced in 75 keV MARS images and artifacts completely invisible in 7 lesions. The number of diagnosable images (score ≥3) was significantly more in the 75-keV MARS group (28/45), and the 75-keV group (22/45) than in the 120-kVp group (11/45) (p improve image quality, even to a state of being

  8. Dynamic multiphoton imaging of acellular dermal matrix scaffolds seeded with mesenchymal stem cells in diabetic wound healing.

    Science.gov (United States)

    Chu, Jing; Shi, Panpan; Deng, Xiaoyuan; Jin, Ying; Liu, Hao; Chen, Maosheng; Han, Xue; Liu, Hanping

    2018-03-25

    Significantly effective therapies need to be developed for chronic nonhealing diabetic wounds. In this work, the topical transplantation of mesenchymal stem cell (MSC) seeded on an acellular dermal matrix (ADM) scaffold is proposed as a novel therapeutic strategy for diabetic cutaneous wound healing. GFP-labeled MSCs were cocultured with an ADM scaffold that was decellularized from normal mouse skin. These cultures were subsequently transplanted as a whole into the full-thickness cutaneous wound site in streptozotocin-induced diabetic mice. Wounds treated with MSC-ADM demonstrated an increased percentage of wound closure. The treatment of MSC-ADM also greatly increased angiogenesis and rapidly completed the reepithelialization of newly formed skin on diabetic mice. More importantly, multiphoton microscopy was used for the intravital and dynamic monitoring of collagen type I (Col-I) fibers synthesis via second harmonic generation imaging. The synthesis of Col-I fibers during diabetic wound healing is of great significance for revealing wound repair mechanisms. In addition, the activity of GFP-labeled MSCs during wound healing was simultaneously traced via two-photon excitation fluorescence imaging. Our research offers a novel advanced nonlinear optical imaging method for monitoring the diabetic wound healing process while the ADM and MSCs interact in situ. Schematic of dynamic imaging of ADM scaffolds seeded with mesenchymal stem cells in diabetic wound healing using multiphoton microscopy. PMT, photo-multiplier tube. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. Evaluation of some engineering properties of cucumber (Cucumis sativus L. seeds and kernels based on image processing

    Directory of Open Access Journals (Sweden)

    Amir Hossein Mirzabe

    2017-12-01

    Full Text Available In this study, a method based on digital image processing was employed to investigate effect of the moisture content on gravimetrical and frictional properties of the cucumber seeds and kernels. This research indicated that the application of visual machines and the image processing could be a rapid method that enables accurate measurement of the dimensional parameters of two varieties of this product meticulously. The length, width, and thickness of the seeds of Rashid variety ranged from 6.40 to 9.07, 2.91 to 4.21 and 0.65 to1.50 mm, respectively; the corresponding value of Negin variety ranged from 6.89 to 9.07, 2.49 to 4.21, and 0.69 to 1.68 mm, respectively. Kernel and shell ratios of the Rashid variety were found to be 69.811 and 30.189%, respectively; the corresponding values of the Negin variety were found to be 72.727 and 27.273%, respectively. Despite the bulk densities of the two varieties of cucumber seeds and kernels that decreased with the moisture content, true density and porosity of the two varieties of cucumber seeds and kernels increased. As the moisture content increased from 5.04 to 21.12% (d.b, the angle of the static friction of the seeds of Negin variety increased from 30.39° to 37.18°, 28.83° to 34.99°, 24.37° to 30.04°, and 15.77° to 19.57° for wood, rubber, iron, and galvanized, respectively,; the corresponding value of the Negin variety increased from 21.12° to 27.16°, 24.43° to 31.41°, 19.90° to 25.59° and 14.09° to 18.12° as the moisture content increased from 5.02 to 21.02% (d.b. Keywords: Image processing, Physical and mechanical properties, Cucumber

  10. Optimum timing for image-based dose evaluation of 125I and 103Pd prostate seed implants

    International Nuclear Information System (INIS)

    Yue Ning; Chen Zhe; Peschel, Richard; Dicker, Adam P.; Waterman, Frank M.; Nath, Ravinder

    1999-01-01

    Purpose/Objective: Image-based dose evaluation of permanent brachytherapy implants for prostate cancer is important for optimal patient management after implantation. Because of edema caused by the surgical procedure in the implantation, if the dose evaluation is based on the images obtained too early after implantation, dose coverage will usually be underestimated. Conversely, if the images are obtained too late, the dose coverage will be overestimated. This study uses a biomathematical model to simulate edema and its resolution on 29 patients, so that the optimum time to obtain image scans and perform dose evaluation can be investigated and estimated. Methods and Materials: Edema of a prostate and its resolution has been shown to follow an exponential function V(t) = V(0)(1 + ΔV[e -0.693t/Te - 1]) where ΔV is the initial relative increase in the prostate volume due to edema (and is related to edema magnitude), and T e (edema half-life) is the time for the edema to decrease by half in volume. In this study, edema was simulated by increasing the volume of preimplant prostate (obtained from ultrasound volume study) to a given magnitude of edema. Similarly, the locations of planned seeds were changed to their corresponding locations in the edematous prostate proportionally. The edema was then allowed to resolve according to the exponential function. The correct dose distribution was calculated by taking into account the dynamic variations of the prostate volume, seed locations, and source strengths with respect to time. Dose volume histograms (DVHs) were then generated from this dose distribution. The conventional postimplant DVHs, which assume the prostate volume and seed locations are as in the image scans and constant in time, were also calculated based on the simulated image scans for various days postimplantation. The conventional DVHs of prostate on various days after implantation were compared to the DVH calculated assuming dynamic conditions. The optimum

  11. SU-E-J-215: Towards MR-Only Image Guided Identification of Calcifications and Brachytherapy Seeds: Application to Prostate and Breast LDR Implant Dosimetry

    Energy Technology Data Exchange (ETDEWEB)

    Elzibak, A; Fatemi-Ardekani, A; Soliman, A; Mashouf, S; Safigholi, H; Ravi, A; Morton, G; Song, WY [Sunnybrook Health Sciences Centre, University of Toronto, Toronto, Ontario (Canada); Han, D [Sunnybrook Health Sciences Centre, University of Toronto, Toronto, Ontario (Canada); University of California, San Diego, La Jolla, CA (United States)

    2015-06-15

    Purpose: To identify and analyze the appearance of calcifications and brachytherapy seeds on magnitude and phase MRI images and to investigate whether they can be distinguished from each other on corrected phase images for application to prostate and breast low dose rate (LDR) implant dosimetry. Methods: An agar-based gel phantom containing two LDR brachytherapy seeds (Advantage Pd-103, IsoAid, 0.8mm diameter, 4.5mm length) and two spherical calcifications (large: 7mm diameter and small: 4mm diameter) was constructed and imaged on a 3T Philips MR scanner using a 16-channel head coil and a susceptibility weighted imaging (SWI) sequence (2mm slices, 320mm FOV, TR/ TE= 26.5/5.3ms, 15 degree flip angle). The phase images were unwrapped and corrected using a 32×32, 2D Hanning high pass filter to remove background phase noise. Appearance of the seeds and calcifications was assessed visually and quantitatively using Osirix (http://www.osirix-viewer.com/). Results: As expected, calcifications and brachytherapy seeds appeared dark (hypointense) relative to the surrounding gel on the magnitude MRI images. The diameter of each seed without the surrounding artifact was measured to be 0.1 cm on the magnitude image, while diameters of 0.79 and 0.37 cm were measured for the larger and smaller calcifications, respectively. On the corrected phase images, the brachytherapy seeds and the calcifications appeared bright (hyperintense). The diameter of the seeds was larger on the phase images (0.17 cm) likely due to the dipole effect. Conclusion: MRI has the best soft tissue contrast for accurate organ delineation leading to most accurate implant dosimetry. This work demonstrated that phase images can potentially be useful in identifying brachytherapy seeds and calcifications in the prostate and breast due to their bright appearance, which helps in their visualization and quantification for accurate dosimetry using MR-only. Future work includes optimizing phase filters to best identify

  12. Influence of harvesting and processing methods on organic viability of soybean seed

    Directory of Open Access Journals (Sweden)

    Đukanović Lana

    2000-01-01

    Full Text Available Organic viability of soybean seed for three soybean varieties - elite (Bosa, ZPS 015 and Nena depending on methods of manipulation with seeds during harvesting and processing phase were determined in this paper. Trial was conducted in Zemun Polje during 1999; manual and mechanized harvesting or processing methods were applied. Seed germination was tested using ISTA methods (Standard method and Cold test. Following parameters were evaluated: germination viability, germination, rate-speed of emergence, length of hypocotile and main root Rate-speed of emergence was based on number of emerged plants per day. Length of hypocotile or root and percent of germination determined vigour index. Based on obtained results it maybe concluded that methods of seed manipulation during harvesting or processing phase were influenced on soybean seed quality parameters evaluated. Ways of seed manipulation - methods evaluated were influenced organic viability of soybean seed by decreasing germination viability, total germination and length of main root.

  13. Cover cropping in Vitis vinifera L. cv. Manto Negro vineyards under Mediterranean conditions: effects on plant vigour, yield and grape quality

    Directory of Open Access Journals (Sweden)

    Alícia Pou

    2011-12-01

    Significance and impact of the study: This study showed that the use of specific cover crops in vineyards under Mediterranean climates helps to reduce vegetative vigour. Nevertheless, yield reduction and slight quality improvement suggest that cover crops should be adjusted in order to reduce competition for water and thus prevent these negative effects of water scarcity.

  14. 14CO2-fixation and nitrate reductase activity in vivo in relation to hybrid vigour in maize

    International Nuclear Information System (INIS)

    Balasubramanian, V.; Shanthakumari, P.; Sinha, S.K.

    1977-01-01

    Dry matter accumulation in maize shoots, leaf area, 14 CO 2 -fixation and nitrate reductase activity in vivo were measured in the field grown heterotic hybrid CM 400x CM 300 and its inbred parents CM 300 and CM 400 from seedling to maturity. Rates of dry matter accumulation and leaf area development were higher in the hybrid during the initial vegetative phase than in the inbreds. The hybrid had more absolute level of 14 CO 2 -fixation and nitrate reductase activity, although the rates of these processes on unit weight basis were not higher than those of inbreds. It is concluded that the rapid development of leaf area in hybrids during the early stages of vegetative growth is probably important for hybrid vigour. (author)

  15. Role of nano-range amphiphilic polymers in seed quality enhancement of soybean and imidacloprid retention capacity on seed coatings.

    Science.gov (United States)

    Adak, Totan; Kumar, Jitendra; Shakil, Najam A; Pandey, Sushil

    2016-10-01

    Nano-size and wide-range solubility of amphiphilic polymers (having both hydrophilic and hydrophobic blocks) can improve uniformity in seed coatings. An investigation was carried out to assess the positive effect of amphiphilic polymers over hydrophilic or hydrophobic polymers as seed coating agents and pesticide carriers. Amphiphilic polymers with 127.5-354 nm micelle size were synthesized in the laboratory using polyethylene glycols and aliphatic di-acids. After 6 months of storage, germination of uncoated soybean seeds decreased drastically from 97.80 to 81.55%, while polymer-coated seeds showed 89.44-95.92% germination. Similarly, vigour index-1 was reduced from 3841.10 to 2813.06 for control seeds but ranged from 3375.59 to 3844.60 for polymer-coated seeds after 6 months. The developed imidacloprid formulations retained more pesticide on soybean seed coatings than did a commercial formulation (Gaucho(®) 600 FS). The time taken for 50% release of imidacloprid from seed coatings in water was 7.12-9.11 h for the developed formulations and 0.41 h for the commercial formulation. Nano-range amphiphilic polymers can be used to protect soybean seeds from ageing. Formulations as seed treatments may produce improved and sustained efficacy with minimum environmental contamination. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  16. Measurement of the density profile of pure and seeded molecular beams by femtosecond ion imaging

    NARCIS (Netherlands)

    Meng, C.; Janssen, M.H.M.

    2015-01-01

    Here, we report on femtosecond ion imaging experiments to measure the density profile of a pulsed supersonic molecular beam. Ion images are measured for both a molecular beam and bulk gas under identical experimental conditions via femtosecond multiphoton ionization of Xe atoms. We report the

  17. The Moving Group Targets of the SEEDS High-contrast Imaging Survey of Exoplanets and Disks: Results and Observations from the First Three Years

    NARCIS (Netherlands)

    Brandt, T.D.; et al., [Unknown; Thalmann, C.

    2014-01-01

    We present results from the first three years of observations of moving group (MG) targets in the Strategic Exploration of Exoplanets and Disks with Subaru (SEEDS) high-contrast imaging survey of exoplanets and disks using the Subaru telescope. We achieve typical contrasts of ~105 at 1'' and ~106

  18. Using of a combined approach by biochemical and image analysis to develop a new method to estimate seed maturity stage for Bordeaux area grapevine

    Directory of Open Access Journals (Sweden)

    Amélie RABOT

    2017-03-01

    Full Text Available Aim: The importance of phenolic maturity (depending on tannins and anthocyans of the grape is crucial at harvest and determines the final quality of wine. The work presented here aims to characterize the evolution of phenolic maturity of seeds for 3 varieties combining macroscopic analysis and biochemical analyzes of these tannins at phenological stages of interest. Methods and results: Using R software for macroscopic analyzes have been shown that the color varies dramatically (from green to dark brown in the two months between the bunch closure and maturity. Biochemical analysis (HPLC measurement shows that tannins in seeds are increasing from bunch closure to early veraison and decrease after this step until maturity. Conclusion: All together these results have shown that the color variation is correlated to the tannins content in the seeds. Significance of the study: Nowadays, no easy ways of prediction of phenolic maturity are known. The aim of this work is to use these results (usually considered independently to have an knowledge of seed level of phenolic maturity necessary without biochemical analysis to establish a date of great harvest for the most favorable conditions for the extraction of tannins required for the organoleptic quality of a wine .The originality of this work is to use the combined visual seeds and its biochemical composition in tannins (correlation established by CPA. Forward, these results will help to develop a decision support tool based on simply system to acquiring seed image easily usable by winemakers.

  19. Detection of cucumber green mottle mosaic virus-infected watermelon seeds using short wave infrared (SWIR) hyperspectral imaging system

    Science.gov (United States)

    The cucurbit diseases caused by cucumber green mottle mosaic virus (CGMMV) have led to a serious problem to growers and seed producers because it is difficult to prevent spreading through causal agent of seeds. Conventional detection methods for infected seed such as a biological, serological, and m...

  20. Visualisation of abscisic acid and 12-oxo-phytodienoic acid in immature Phaseolus vulgaris L. seeds using desorption electrospray ionisation-imaging mass spectrometry

    Science.gov (United States)

    Enomoto, Hirofumi; Sensu, Takuya; Sato, Kei; Sato, Futoshi; Paxton, Thanai; Yumoto, Emi; Miyamoto, Koji; Asahina, Masashi; Yokota, Takao; Yamane, Hisakazu

    2017-02-01

    The plant hormone abscisic acid (ABA) and the jasmonic acid related-compound 12-oxo-phytodienoic acid (OPDA) play crucial roles in seed development, dormancy, and germination. However, a lack of suitable techniques for visualising plant hormones has restricted the investigation of their biological mechanisms. In the present study, desorption electrospray ionisation-imaging mass spectrometry (DESI-IMS), a powerful tool for visualising metabolites in biological tissues, was used to visualise ABA and OPDA in immature Phaseolus vulgaris L. seed sections. The mass spectra, peak values and chemical formulae obtained from the analysis of seed sections were consistent with those determined for ABA and OPDA standards, as were the precursor and major fragment ions observed in tandem mass spectrometry (MS/MS) imaging. Furthermore, the precursor and fragment ion images showed similar distribution patterns. In addition, the localisation of ABA and OPDA using DESI-IMS was confirmed using liquid chromatography-MS/MS (LC-MS/MS). The results indicated that ABA was mainly distributed in the radical and cotyledon of the embryo, whereas OPDA was distributed exclusively in external structures, such as the hilum and seed coat. The present study is the first to report the visualisation of plant hormones using IMS, and demonstrates that DESI-IMS is a promising technique for future plant hormone research.

  1. A modified Seeded Region Growing algorithm for vessel segmentation in breast MRI images for investigating the nature of potential lesions

    Science.gov (United States)

    Glotsos, D.; Vassiou, K.; Kostopoulos, S.; Lavdas, El; Kalatzis, I.; Asvestas, P.; Arvanitis, D. L.; Fezoulidis, I. V.; Cavouras, D.

    2014-03-01

    The role of Magnetic Resonance Imaging (MRI) as an alternative protocol for screening of breast cancer has been intensively investigated during the past decade. Preliminary research results have indicated that gadolinium-agent administrative MRI scans may reveal the nature of breast lesions by analyzing the contrast-agent's uptake time. In this study, we attempt to deduce the same conclusion, however, from a different perspective by investigating, using image processing, the vascular network of the breast at two different time intervals following the administration of gadolinium. Twenty cases obtained from a 3.0-T MRI system (SIGNA HDx; GE Healthcare) were included in the study. A new modification of the Seeded Region Growing (SRG) algorithm was used to segment vessels from surrounding background. Delineated vessels were investigated by means of their topology, morphology and texture. Results have shown that it is possible to estimate the nature of the lesions with approximately 94.4% accuracy, thus, it may be claimed that the breast vascular network does encodes useful, patterned, information, which can be used for characterizing breast lesions.

  2. Real-time imaging of {sup 35}S-sulfate uptake in a rape seed plant

    Energy Technology Data Exchange (ETDEWEB)

    Nakanishi, T.M.; Yamawaki, M.; Ishibashi, H.; Tanoi, K. [Tokyo Univ. (Japan). Lab. of Radioisotope Plant Physiology

    2011-07-01

    We present real-time images of {sup 35}S-sulfate uptake in a rapeseed plant visualized by the system we developed. In the leaves of rapeseed plants, {sup 35}S accumulated in higher amounts and more rapidly in the more developed leaves. This real-time imaging system can be used to visualize the movement of both {sup 35}S and {sup 32}P in the same plant. In the pods of rapeseed, images of {sup 35}S show that {sup 35}S accumulated mostly in the terminal parts; on the other hand {sup 32}P, when applied as {sup 32}P-phosphoric acid, accumulated in the middle part of the pods. (orig.)

  3. Electron beam irradiation: a novel technology to enhance the quality of soybean seeds

    International Nuclear Information System (INIS)

    Bhalla, Shashi; Srinivasan, K.; Singh, Subadas; Thakur, Manju; Sharma, S.K.; Pramod, R.; Dwivedi, J.; Bapna, S.C.

    2010-01-01

    Soybean seeds, rich in protein and oil, maintain their germinability only for short durations under ambient conditions. Loss of viability of stored seeds often hampers soybean production in harsh environments worldwide. Physiological factors favored by high temperature and high moisture content accelerate the seed deterioration in the tropics. Several chemical and physical treatments are being used to enhance quality. Irradiation is a novel technology for food preservation and is gaining importance all over the world. Low doses of irradiation bring about improvement in quality of food/seeds, which can be beneficial in several ways. Electron Beam (EB) irradiation is a new approach in this area. The objective of present study was to investigate the effect of EB irradiation in enhancing the quality of low vigour soybean seeds

  4. (Heckel) seeds

    African Journals Online (AJOL)

    UTILISATEUR

    Garcinia kola seeds to six different hormonal pre-germination treatments. This consisted of ... Thus, seed dormancy in this case is not a coat- imposed .... development of the cultivation of the species. The cause .... Hormonal regulation of seed ...

  5. Hybridization between crops and wild relatives: the contribution of cultivated lettuce to the vigour of crop-wild hybrids under drought, salinity and nutrient deficiency conditions.

    Science.gov (United States)

    Uwimana, Brigitte; Smulders, Marinus J M; Hooftman, Danny A P; Hartman, Yorike; van Tienderen, Peter H; Jansen, Johannes; McHale, Leah K; Michelmore, Richard W; van de Wiel, Clemens C M; Visser, Richard G F

    2012-10-01

    With the development of transgenic crop varieties, crop-wild hybridization has received considerable consideration with regard to the potential of transgenes to be transferred to wild species. Although many studies have shown that crops can hybridize with their wild relatives and that the resulting hybrids may show improved fitness over the wild parents, little is still known on the genetic contribution of the crop parent to the performance of the hybrids. In this study, we investigated the vigour of lettuce hybrids using 98 F(2:3) families from a cross between cultivated lettuce and its wild relative Lactuca serriola under non-stress conditions and under drought, salinity and nutrient deficiency. Using single nucleotide polymorphism markers, we mapped quantitative trait loci associated with plant vigour in the F(2:3) families and determined the allelic contribution of the two parents. Seventeen QTLs (quantitative trait loci) associated with vigour and six QTLs associated with the accumulation of ions (Na(+), Cl(-) and K(+)) were mapped on the nine linkage groups of lettuce. Seven of the vigour QTLs had a positive effect from the crop allele and six had a positive effect from the wild allele across treatments, and four QTLs had a positive effect from the crop allele in one treatment and from the wild allele in another treatment. Based on the allelic effect of the QTLs and their location on the genetic map, we could suggest genomic locations where transgene integration should be avoided when aiming at the mitigation of its persistence once crop-wild hybridization takes place.

  6. SU-F-T-40: Can CBCT Images Be Used for Volume Studies of Prostate Seed Implants for Boost Treatment?

    Energy Technology Data Exchange (ETDEWEB)

    Xu, H; Lee, S; Diwanji, T; Amin, P; Krudys, K; Guerrero, M [University of Maryland School of Medicine, Baltimore, MD (United States)

    2016-06-15

    Purpose: In our clinic, the planning CT is used for definitive and boost low-dose-rate (LDR) brachytherapy treatments to determine the ultrasound volume in the operating room (OR) at the time of the implant. While the CT overestimation of OR volume is known, a larger estimation discrepancy has been observed for boost treatments. A possible reason is the prostate size reduction during EBRT for boost patients. Since cone-beam CT (CBCT) is often used as routine imaging guidance of EBRT, this prostate volume change may be captured. This study investigates if CBCT taken during EBRT includes the volume change information and therefore beats CT in estimating the prostate OR volumes. Methods: 9 prostate patients treated with EBRT (45Gy in 1.8Gy per fractions to the whole pelvis) and I-125 seed implants (108Gy) were involved in this study. During EBRT, CBCT image guidance was performed on a weekly basis. For each patient, the prostate volumes on the first and the last available CBCT images were manually contoured by a physician. These volumes were then compared to each other and with the contoured volumes from the planning CT and from the ultrasound images in the OR. Results: The first and the last CBCT images did not show significant prostate volume change. Their average +/− standard deviation of prostate volumes were 24.4cc+/−14.6cc and 29.9cc+/−16.1cc, respectively (T-test p=0.68). The ratio of the OR volume to the last CBCT (0.71+/−0.21) was not significantly different from the ratio of OR volumes to the planning CT (0.61+/−0.13) (p=0.25). Conclusion: In this study, CBCT does not show significant prostate volume changes during EBRT. CBCT and CT volumes are quite consistent and no improvement of volume estimation using CBCT is observed. The advantage of CBCT as a replacement of CT for volume study of boost LDR brachytherapy is limited.

  7. Comparison of daily megavoltage electronic portal imaging or kilovoltage imaging with marker seeds to ultrasound imaging or skin marks for prostate localization and treatment positioning in patients with prostate cancer

    International Nuclear Information System (INIS)

    Serago, Christopher F.; Buskirk, Steven J.; Igel, Todd C.; Gale, Ashley A.; Serago, Nicole E.; Earle, John D.

    2006-01-01

    Purpose: To compare the accuracy of imaging modalities, immobilization, localization, and positioning techniques in patients with prostate cancer. Methods and Materials: Thirty-five patients with prostate cancer had gold marker seeds implanted transrectally and were treated with fractionated radiotherapy. Twenty of the 35 patients had limited immobilization; the remaining had a vacuum-based immobilization. Patient positioning consisted of alignment with lasers to skin marks, ultrasound or kilovoltage X-ray imaging, optical guidance using infrared reflectors, and megavoltage electronic portal imaging (EPI). The variance of each positioning technique was compared to the patient position determined from the pretreatment EPI. Results: With limited immobilization, the average difference between the skin marks' laser position and EPI pretreatment position is 9.1 ± 5.3 mm, the average difference between the skin marks' infrared position and EPI pretreatment position is 11.8 ± 7.2 mm, the average difference between the ultrasound position and EPI pretreatment position is 7.0 ± 4.6 mm, the average difference between kV imaging and EPI pretreatment position is 3.5 ± 3.1 mm, and the average intrafraction movement during treatment is 3.4 ± 2.7 mm. For the patients with the vacuum-style immobilization, the average difference between the skin marks' laser position and EPI pretreatment position is 10.7 ± 4.6 mm, the average difference between kV imaging and EPI pretreatment position is 1.9 ± 1.5 mm, and the average intrafraction movement during treatment is 2.1 ± 1.5 mm. Conclusions: Compared with use of skin marks, ultrasound imaging for positioning provides an increased degree of agreement to EPI-based positioning, though not as favorable as kV imaging fiducial seeds. Intrafraction movement during treatment decreases with improved immobilization

  8. EPR imaging and HPLC characterization of the pigment-based organic free radical in black soybean seeds.

    Science.gov (United States)

    Nakagawa, Kouichi; Maeda, Hayato

    2017-02-01

    We investigated the location and distribution of paramagnetic species in dry black, brown, and yellow (normal) soybean seeds using electron paramagnetic resonance (EPR), X-band (9 GHz) EPR imaging (EPRI), and HPLC. EPR primarily detected two paramagnetic species in black soybean. These two different radical species were assigned as stable organic radical and Mn 2+  species based on the g values and hyperfine structures. The signal from the stable radical was noted at g ≈ 2.00 and was relatively strong and stable. Subsequent noninvasive two-dimensional (2D) EPRI of the radical present in black soybean revealed that the stable radical was primarily located in the pigmented region of the soybean coat, with very few radicals observed in the soybean cotyledon (interior). Pigments extracted from black soybean were analyzed using HPLC. The major compound was found to be cyanidin-3-glucoside. Multi-EPR and HPLC results indicate that the stable radical was only found within the pigmented region of the soybean coat, and it could be cyanidin-3-glucoside or an oxidative decomposition product.

  9. Genetic effects on seed quality in diallel crosses of popcorn

    Directory of Open Access Journals (Sweden)

    Pablo Diego Silva Cabral

    2013-12-01

    Full Text Available The failure to obtain the ideal stand is one of the causes of decreased crop yields, in this sense it is important to investigate the genetic effects related to seed quality. The aim of this study was to measure the general combining ability (GCA, the specific combining ability (SCA and the reciprocal effects (RE for popcorn seed quality in addition to evaluate the association between germination and vigor tests with field emergencein order to identify hybrids with better germination and vigour. Ten inbred lines were evaluated using a complete diallel cross with reciprocals. Seed quality was measured by germination tests (GT and by modified cold vigour tests (MCV. In the GT, the numbers of strong normal seedlings (SNS, weak normal (WNS, abnormal (AS and ungerminated seeds (UGS were counted. In the MCV, the numbers of normal seedlings (NPC, abnormal (ASC and ungerminated seeds (UGSC were counted, and the plants' dry matter (DM was measured. Analysis of variance for GCA, SCA and RE were significant for all variables. The quadratic components for SCA were higher than those for GCA for the SNS, AS, UGS, ASC and UGSC traits, which indicates higher significance for the non-additive effects. The most favourable GCA estimates, were found in lines P3 and L70. The best hybrids were P1xL70, P3xP6 and P8xL70. The RE results showed that L70 and P3 should be used as the female parent in the P1xL70 and P3xP6 hybrid crosses, respectively. The MCV was the test that was most strongly correlated with field emergence, with a magnitude of 0.667.

  10. Seed Size And Physiological Quality Of Three Cultivars Of Cotton

    Directory of Open Access Journals (Sweden)

    Luiz Cesar Lopes Filho

    2018-05-01

    Full Text Available Cotton is one of the most important crops, both in the national context and worldwide. Being that, its fiber, constitutes an important raw material for the textile industry. The process of improving cottonseeds, favoured cultivars with smaller seeds. The goal of this work was to evaluate three cultivars of cottonseeds with different sizes through vigour tests. The work was developed in the seeds laboratory and the post-harvest Laboratory of plant products of the Instituto Federal Goiano – Campus Rio Verde. Cottonseeds of 3 cultivars were used (FiberMax 913 GLT, FiberMax 910 and DeltaPine 1648 B2RF, obtained in the crop of 2014 in the city of Primavera do Leste, Mato Grosso. The experimental design was entirely randomized, with eight replications of 50 seeds. Determinations of moisture content and weight of a hundred seeds, germination test and germination velocity index, emergency test, and emergency speed index, electrical conductivity and accelerated aging were conducted. The dimensions of the size of the seeds (length, width and thickness were also determined, with the aid of digital caliper, and the weight of the seeds, with the aid of a balance of precision of resolution 0, 001g. The data was subjected to analysis of variance and the averages compared to the Tukey test at 5% probability. There was no clear relationship between the size of the seed and its physiological performance in assessed cultivars/batches, making it necessary more studies that can prove that type of association.

  11. Canopy structure and physiology related to rootstock vigour in early-ripening peach cultivar Flordastar

    International Nuclear Information System (INIS)

    Motisi, A.; Grutta, I.; Pernice, F.; Caruso, T.

    2005-01-01

    Canopy architectural and eco-physiological traits were measured on five-year-old early-ripening peach cv Flordastar trees grafted on GF 677 and MrS 2/5 rootstocks. Data are reported both on measurements performed directly on the trees, for branches and twigs characters, and on the fractal dimension (D), estimated by the 'box counting' method taken from digital images of Winter-dormant trees, adopted as an indicator of canopy complexity. Results are discussed in relation to the modification of the canopy microclimate as a consequence of the effects of rootstock on tree architecture and water consumption, the latter measured by using sap flow (HPV) probes. A lower degree of canopy complexity was observed in trees grafted onto MrS 2/5 and this, in turn, was related to a higher degree of aerodynamic contact of the tree with the atmosphere (expressed in terms of leaf boundary conductance) and to a higher solar radiation intensity along the canopy profile. These differences did not affect fruit quality in terms of size, red skin over-colour and soluble solid content. In MrS 2/5, the higher light availability at all levels along canopy profile was related to a moderate water deficit status, even under full-irrigation conditions, as evidenced by the lower stem water potential (below -1.3 MPa) and by a lower transpiration rate (about one-half of the values observed on GF 677). At tree-level, MrS 2/5 had a daily water consumption that, also in relation to the lower leaf area per tree, resulted as low as 25% of the values observed on GF 677. The latter, even carrying a significantly higher leaf area and higher water consumption, never showed apparent symptoms of water deficit [it

  12. Complementary method of analyzing the quality of the implant I-125 seeds for prostate brachytherapy using ultrasound imaging post-implant

    International Nuclear Information System (INIS)

    Jimenez Dominguez, M.; Carrasco Herrera, M.; Baeza Trujillo, M.; Herrador Cordoba, M.

    2011-01-01

    In this paper we propose a complementary method based on Longitudinal mode ultrasound images acquired the same day of surgery, at the end of the implant. This option will allow us to evaluate the dosimetry end of treatment with the patient in the same position he was planning and to the rectum and bladder just as full. This will permit the identification of bodies and the seeds of interest more easily and will have a reference with which to compare one month later, when the CT images can also detect whether there has been some migration.

  13. GmSALT3, which Confers Improved Soybean Salt Tolerance in the Field, Increases Leaf Cl– Exclusion prior to Na+ Exclusion but does not Improve Early Vigour under Salinity

    Directory of Open Access Journals (Sweden)

    Ying Liu

    2016-09-01

    Full Text Available Soil salinity reduces soybean growth and yield. The recently identified GmSALT3 (Glycine max salt Tolerance-associated gene on chromosome 3 has the potential to improve soybean yields in salinized conditions. To evaluate the impact of GmSALT3 on soybean performance under saline or non-saline conditions, three sets of near isogenic lines (NILs, with genetic similarity 95.6-99.3% between each pair of NIL-T and NIL-S, were generated from a cross between two varieties, 85-140 (salt-sensitive, S and Tiefeng 8 (salt-tolerant, T by using marker-assisted selection. Each NIL; 782-T, 820-T and 860-T, contained a common ~1000 kb fragment on chromosome 3 where GmSALT3 was located. We show that GmSALT3 does not contribute to an improvement in seedling emergence rate or early vigour under salt stress. However, when 12-day-old seedlings were exposed to NaCl stress, the NIL-T lines accumulated significantly less leaf Na+ compared with their corresponding NIL-S, while no significant difference of K+ concentration was observed between NIL-T and NIL-S; the magnitude of Na+ accumulation within each NIL-T set was influenced by the different genetic backgrounds. In addition, NIL-T lines accumulated less Cl– in the leaf and more in the root prior to any difference in Na+; in the field they accumulated less pod wall Cl– than the corresponding NIL-S lines. Under non-saline field conditions, no significant differences were observed for yield related traits within each pair of NIL-T and NIL-S lines, indicating there was no yield penalty for having the GmSALT3 gene. In contrast, under saline field conditions the NIL-T lines had significantly greater plant seed weight and 100-seed weight than the corresponding NIL-S lines, meaning GmSALT3 conferred a yield advantage to soybean plants in salinized fields. Our results indicated that GmSALT3 mediated regulation of both Na+ and Cl– accumulation in soybean, and contributes to improved soybean yield through maintaining a

  14. Oxyradical accumulation and rapid deterioration of soybean seeds due to field weathering.

    Science.gov (United States)

    Yadav, Sanjeev; Bhatia, V S; Guruprasad, K N

    2006-02-01

    The effect of field weathering on oxyradical accumulation and subsequent changes were studied in the seeds of soybean [Glycine max (L.) Merr.] cv. JS 71-05. Electron spin resonance (ESR) quantification of oxyradical revealed that field weathering plays an important role in acceleration of their accumulation. One week of weathering increased the accumulation of oxyradicals to almost 2-fold and triggered the deteriorative cascade, by enhancing the lipid peroxidation and membrane perturbation, leading to cell death in seed tissues and poor germinability and vigour of soybean seeds. Thus, the weather conditions at the time of physiological maturity to harvesting of crop are very crucial and the field weathering plays a critical role for the maintenance of seed quality.

  15. Effects of Cadmium Stress on Seed Germination, Seedling Growth and Seed Amylase Activities in Rice (Oryza sativa

    Directory of Open Access Journals (Sweden)

    Jun-yu HE

    2008-12-01

    Full Text Available Two rice varieties, Xiushui 110 with high cadmium (Cd tolerance and Xiushui 11 with low Cd tolerance were used to study the effects of Cd stress on seed germination, seedling growth and amylase activities. The low cadmium concentration had little effect on seed germination rate. However, cadmium stress could significantly inhibit plumule and radicle growth, especially for radicle growth. Germination index, vigour index, radicle length and amylase activities of Xiushui 11 decreased more significantly with the increasing cadmium level compared with Xiushui 110. The cadmium content in seedlings of Xiushui 11 was higher than that in Xiushui 110 when the cadmium concentration exceeded 5 μmol/L, which caused lower mitotic index in root tips and amylase activities, and more serious cadmium toxicity in Xiushui 11.

  16. Determination of total iron-reactive phenolics, anthocyanins and tannins in wine grapes of skins and seeds based on near-infrared hyperspectral imaging.

    Science.gov (United States)

    Zhang, Ni; Liu, Xu; Jin, Xiaoduo; Li, Chen; Wu, Xuan; Yang, Shuqin; Ning, Jifeng; Yanne, Paul

    2017-12-15

    Phenolics contents in wine grapes are key indicators for assessing ripeness. Near-infrared hyperspectral images during ripening have been explored to achieve an effective method for predicting phenolics contents. Principal component regression (PCR), partial least squares regression (PLSR) and support vector regression (SVR) models were built, respectively. The results show that SVR behaves globally better than PLSR and PCR, except in predicting tannins content of seeds. For the best prediction results, the squared correlation coefficient and root mean square error reached 0.8960 and 0.1069g/L (+)-catechin equivalents (CE), respectively, for tannins in skins, 0.9065 and 0.1776 (g/L CE) for total iron-reactive phenolics (TIRP) in skins, 0.8789 and 0.1442 (g/L M3G) for anthocyanins in skins, 0.9243 and 0.2401 (g/L CE) for tannins in seeds, and 0.8790 and 0.5190 (g/L CE) for TIRP in seeds. Our results indicated that NIR hyperspectral imaging has good prospects for evaluation of phenolics in wine grapes. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Near Infrared Microspectroscopy, Fluorescence Microspectroscopy, Infrared Chemical Imaging and High Resolution Nuclear Magnetic Resonance Analysis of Soybean Seeds, Somatic Embryos and Single Cells

    CERN Document Server

    Baianu, I C; Hofmann, N E; Korban, S S; Lozano, P; You, T; AOCS 94th Meeting, Kansas

    2002-01-01

    Novel methodologies are currently being developed and established for the chemical analysis of soybean seeds, embryos and single cells by Fourier Transform Infrared (FT-IR), Fourier Transform Near Infrared (FT-NIR) Microspectroscopy, Fluorescence and High-Resolution NMR (HR-NMR). The first FT-NIR chemical images of biological systems approaching one micron resolution are presented here. Chemical images obtained by FT-NIR and FT-IR Microspectroscopy are presented for oil in soybean seeds and somatic embryos under physiological conditions. FT-NIR spectra of oil and proteins were obtained for volumes as small as two cubic microns. Related, HR-NMR analyses of oil contents in somatic embryos are also presented here with nanoliter precision. Such 400 MHz 1H NMR analyses allowed the selection of mutagenized embryos with higher oil content (e.g. ~20%) compared to non-mutagenized control embryos. Moreover, developmental changes in single soybean seeds and/or somatic embryos may be monitored by FT-NIR with a precision ...

  18. ROADS CENTRE-AXIS EXTRACTION IN AIRBORNE SAR IMAGES: AN APPROACH BASED ON ACTIVE CONTOUR MODEL WITH THE USE OF SEMI-AUTOMATIC SEEDING

    Directory of Open Access Journals (Sweden)

    R. G. Lotte

    2013-05-01

    Full Text Available Research works dealing with computational methods for roads extraction have considerably increased in the latest two decades. This procedure is usually performed on optical or microwave sensors (radar imagery. Radar images offer advantages when compared to optical ones, for they allow the acquisition of scenes regardless of atmospheric and illumination conditions, besides the possibility of surveying regions where the terrain is hidden by the vegetation canopy, among others. The cartographic mapping based on these images is often manually accomplished, requiring considerable time and effort from the human interpreter. Maps for detecting new roads or updating the existing roads network are among the most important cartographic products to date. There are currently many studies involving the extraction of roads by means of automatic or semi-automatic approaches. Each of them presents different solutions for different problems, making this task a scientific issue still open. One of the preliminary steps for roads extraction can be the seeding of points belonging to roads, what can be done using different methods with diverse levels of automation. The identified seed points are interpolated to form the initial road network, and are hence used as an input for an extraction method properly speaking. The present work introduces an innovative hybrid method for the extraction of roads centre-axis in a synthetic aperture radar (SAR airborne image. Initially, candidate points are fully automatically seeded using Self-Organizing Maps (SOM, followed by a pruning process based on specific metrics. The centre-axis are then detected by an open-curve active contour model (snakes. The obtained results were evaluated as to their quality with respect to completeness, correctness and redundancy.

  19. Estabelecimento de plântulas e desempenho de plantas em resposta ao vigor dos aquênios de girassol Seedlings establishment and plants performance as affected by sunflower achenes vigour

    Directory of Open Access Journals (Sweden)

    Madelon Rodrigues Sá Braz

    2009-10-01

    Full Text Available O vigor das sementes pode influenciar o estabelecimento e o desempenho das plantas, mas o efeito na produção dessas plantas, principalmente, de cultivo anual, cuja colheita é feita na fase reprodutiva, não está completamente esclarecido. O objetivo do trabalho foi avaliar a influência da qualidade fisiológica de aquênios de girassol no estabelecimento das plântulas e no desempenho das plantas. Para tanto, foi instalado um experimento em campo, com três lotes de aquênios de girassol da cultivar 'Embrapa 122 V2000', com distintos níveis de vigor. A população de plantas foi avaliada aos 10, 20, 40, 60, 80 e 100 dias após a semeadura (DAS. Aos 20, 40, 60, 80 e 100DAS, em intervalo de 20 dias, foram avaliados a altura da planta, o número de folhas, o diâmetro de caule e a massa de matéria seca de parte aérea e total. Também aos 100DAS foram avaliados o número de aquênios por planta, a massa de 1000 aquênios, a produção de aquênios (g planta-1 e o rendimento (kg ha-1. Após dois e seis meses da colheita, os aquênios foram submetidos aos testes de germinação, de primeira contagem, de envelhecimento acelerado, de emergência de plântulas em areia, de comprimento de plântula e de condutividade elétrica. Os resultados permitiram concluir que houve efeito favorável do vigor dos aquênios na população de plantas até os 60DAS. A utilização de aquênios vigorosos de girassol favoreceu o desenvolvimento das plantas após 60DAS, e esse efeito persistiu até a produção, na ausência de diferença na população inicial.Seed vigour may influence the establishment and growth of plants. However, the effect on plants production mostly annual crop, which harvesting is done in the reproductive phase, is not clear. The aim of this research was to evaluate the influence of sunflower achenes physiological quality on the seedlings establishment and plant performance. An experiment was installed in the field, with three lots of

  20. Phosphorus Transport in Arabidopsis and Wheat: Emerging Strategies to Improve P Pool in Seeds

    Directory of Open Access Journals (Sweden)

    Mushtak Kisko

    2018-02-01

    Full Text Available Phosphorus (P is an essential macronutrient for plants to complete their life cycle. P taken up from the soil by the roots is transported to the rest of the plant and ultimately stored in seeds. This stored P is used during germination to sustain the nutritional demands of the growing seedling in the absence of a developed root system. Nevertheless, P deficiency, an increasing global issue, greatly decreases the vigour of afflicted seeds. To combat P deficiency, current crop production methods rely on heavy P fertilizer application, an unsustainable practice in light of a speculated decrease in worldwide P stocks. Therefore, the overall goal in optimizing P usage for agricultural purposes is both to decrease our dependency on P fertilizers and enhance the P-use efficiency in plants. Achieving this goal requires a robust understanding of how plants regulate inorganic phosphate (Pi transport, during vegetative growth as well as the reproductive stages of development. In this short review, we present the current knowledge on Pi transport in the model plant Arabidopsis thaliana and apply the information towards the economically important cereal crop wheat. We highlight the importance of developing our knowledge on the regulation of these plants’ P transport systems and P accumulation in seeds due to its involvement in maintaining their vigour and nutritional quality. We additionally discuss further discoveries in the subjects this review discusses substantiate this importance in their practical applications for practical food security and geopolitical applications.

  1. Qualidade fisiológica e sanitária de sementes de melão (Cucumis melo Physiological and health quality of melon (Cucumis melo seeds

    Directory of Open Access Journals (Sweden)

    Marlove Fátima Brião Muniz

    2004-06-01

    Full Text Available O objetivo do trabalho foi avaliar a eficiência de diferentes testes na identificação do nível de vigor e da qualidade sanitária de lotes de sementes de melão (Cucumis melo L.. Foram avaliados quatro lotes de sementes das cultivares Gaúcho e Carvalho, submetidas aos testes de primeira contagem de germinação, emergência em campo, deterioração controlada, envelhecimento acelerado e sanidade. Os resultados indicaram que os testes de deterioração controlada e envelhecimento acelerado apresentam sensibilidade suficiente para avaliação do potencial fisiológico de sementes de melão, mas os testes de avaliação de plântulas não mostraram sensibilidade suficiente para realizar uma estratificação dos lotes pelo vigor. Aspergillus spp. e Fusarium oxysporum foram encontrados associados às sementes.The objective of this work was to evaluate the efficiency of different vigour tests in the identification of vigour levels and health quality of melon (Cucumis melo L. seeds. Four seed lots of Gaúcho and Carvalho cultivars were evaluated. Seeds were submitted to the first germination counting, field emergence, controlled deterioration and aging, cold vigour and health tests. Results indicated that controlled deterioration and accelerated aging tests presented enough sensibility for evaluating the physiological potential of melon seeds. Tests of seedling evaluation did not show enough sensibility to identify hiht quality seed lots. Aspergillus ssp. and Fusarium oxysporum were detected associated with seeds.

  2. Seed quality in informal seed systems

    NARCIS (Netherlands)

    Biemond, P.C.

    2013-01-01

    Keywords: informal seed systems, seed recycling, seed quality, germination, seed pathology, seed health, seed-borne diseases, mycotoxigenic fungi, Fusarium verticillioides, mycotoxins, Vigna unguiculata, Zea mays, Nigeria.

    Seed is a crucial input for agricultural production.

  3. Cytokinins and Expression of SWEET, SUT, CWINV and AAP Genes Increase as Pea Seeds Germinate

    Directory of Open Access Journals (Sweden)

    Paula E. Jameson

    2016-12-01

    Full Text Available Transporter genes and cytokinins are key targets for crop improvement. These genes are active during the development of the seed and its establishment as a strong sink. However, during germination, the seed transitions to being a source for the developing root and shoot. To determine if the sucrose transporter (SUT, amino acid permease (AAP, Sugar Will Eventually be Exported Transporter (SWEET, cell wall invertase (CWINV, cytokinin biosynthesis (IPT, activation (LOG and degradation (CKX gene family members are involved in both the sink and source activities of seeds, we used RT-qPCR to determine the expression of multiple gene family members, and LC-MS/MS to ascertain endogenous cytokinin levels in germinating Pisum sativum L. We show that genes that are actively expressed when the seed is a strong sink during its development, are also expressed when the seed is in the reverse role of being an active source during germination and early seedling growth. Cytokinins were detected in the imbibing seeds and were actively biosynthesised during germination. We conclude that, when the above gene family members are targeted for seed yield improvement, a downstream effect on subsequent seed germination or seedling vigour must be taken into consideration.

  4. seed oils

    African Journals Online (AJOL)

    Timothy Ademakinwa

    processes, production of biodiesel, as lubricant and in deep-frying purposes. They could ... for its juice, nectars and fruit while its seeds are ... Malaysia. The fine seed powder was stored in a plastic container inside a refrigerator at between 4 o.

  5. seed flour

    African Journals Online (AJOL)

    ONOS

    2010-09-06

    Sep 6, 2010 ... and with a nice taste, used for cooking or as lamp oil. The fatty acid ... Pra seeds were obtained from a local market in Nakhon Si Thammarat. Page 2. Table 1. Proximate composition of pra seed flour. Constituent. Percentage ...

  6. High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.).

    Science.gov (United States)

    Thomas, C L; Graham, N S; Hayden, R; Meacham, M C; Neugebauer, K; Nightingale, M; Dupuy, L X; Hammond, J P; White, P J; Broadley, M R

    2016-04-06

    Root traits can be selected for crop improvement. Techniques such as soil excavations can be used to screen root traits in the field, but are limited to genotypes that are well-adapted to field conditions. The aim of this study was to compare a low-cost, high-throughput root phenotyping (HTP) technique in a controlled environment with field performance, using oilseed rape (OSR;Brassica napus) varieties. Primary root length (PRL), lateral root length and lateral root density (LRD) were measured on 14-d-old seedlings of elite OSR varieties (n = 32) using a 'pouch and wick' HTP system (∼40 replicates). Six field experiments were conducted using the same varieties at two UK sites each year for 3 years. Plants were excavated at the 6- to 8-leaf stage for general vigour assessments of roots and shoots in all six experiments, and final seed yield was determined. Leaves were sampled for mineral composition from one of the field experiments. Seedling PRL in the HTP system correlated with seed yield in four out of six (r = 0·50, 0·50, 0·33, 0·49;P emergence in three out of five (r = 0·59, 0·22, 0·49;P emergence, general early vigour or yield in the field. Associations between PRL and field performance are generally related to early vigour. These root traits might therefore be of limited additional selection value, given that vigour can be measured easily on shoots/canopies. In contrast, LRD cannot be assessed easily in the field and, if LRD can improve nutrient uptake, then it may be possible to use HTP systems to screen this trait in both elite and more genetically diverse, non-field-adapted OSR. © The Author 2016. Published by Oxford University Press on behalf of the Annals of Botany Company.

  7. Robotic seeding

    DEFF Research Database (Denmark)

    Pedersen, Søren Marcus; Fountas, Spyros; Sørensen, Claus Aage Grøn

    2017-01-01

    Agricultural robotics has received attention for approximately 20 years, but today there are only a few examples of the application of robots in agricultural practice. The lack of uptake may be (at least partly) because in many cases there is either no compelling economic benefit......, or there is a benefit but it is not recognized. The aim of this chapter is to quantify the economic benefits from the application of agricultural robots under a specific condition where such a benefit is assumed to exist, namely the case of early seeding and re-seeding in sugar beet. With some predefined assumptions...... with regard to speed, capacity and seed mapping, we found that among these two technical systems both early seeding with a small robot and re-seeding using a robot for a smaller part of the field appear to be financially viable solutions in sugar beet production....

  8. Updated Methods for Seed Shape Analysis

    Directory of Open Access Journals (Sweden)

    Emilio Cervantes

    2016-01-01

    Full Text Available Morphological variation in seed characters includes differences in seed size and shape. Seed shape is an important trait in plant identification and classification. In addition it has agronomic importance because it reflects genetic, physiological, and ecological components and affects yield, quality, and market price. The use of digital technologies, together with development of quantification and modeling methods, allows a better description of seed shape. Image processing systems are used in the automatic determination of seed size and shape, becoming a basic tool in the study of diversity. Seed shape is determined by a variety of indexes (circularity, roundness, and J index. The comparison of the seed images to a geometrical figure (circle, cardioid, ellipse, ellipsoid, etc. provides a precise quantification of shape. The methods of shape quantification based on these models are useful for an accurate description allowing to compare between genotypes or along developmental phases as well as to establish the level of variation in different sets of seeds.

  9. Detection of Watermelon Seeds Exterior Quality based on Machine Vision

    OpenAIRE

    Xiai Chen; Ling Wang; Wenquan Chen; Yanfeng Gao

    2013-01-01

    To investigate the detection of watermelon seeds exterior quality, a machine vision system based on least square support vector machine was developed. Appearance characteristics of watermelon seeds included area, perimeter, roughness, minimum enclosing rectangle and solidity were calculated by image analysis after image preprocess.The broken seeds, normal seeds and high-quality seeds were distinguished by least square support vector machine optimized by genetic algorithm. Compared to the grid...

  10. Seed regulations and local seed systems

    NARCIS (Netherlands)

    Louwaars, N.

    2000-01-01

    Seed regulations have been introduced in most countries based on the development of formal seed production. Concerns about seed quality and about the varietal identity of the seeds have commonly led to seed laws. However, formal regulations are often inappropriate for informal seed systems, which

  11. The Moving Group Targets of the Seeds High-Contrast Imaging Survey of Exoplanets and Disks: Results and Observations from the First Three Years

    Science.gov (United States)

    Brandt, Timothy D.; Kuzuhara, Masayuki; McElwain, Michael W.; Schlieder, Joshua E.; Wisniewski, John P.; Turner, Edwin L.; Carson, J.; Matsuo, T.; Biller, B.; Bonnefoy, M.; hide

    2014-01-01

    We present results from the first three years of observations of moving group (MG) targets in the Strategic Exploration of Exoplanets and Disks with Subaru (SEEDS) high-contrast imaging survey of exoplanets and disks using the Subaru telescope. We achieve typical contrasts of (is) approximately10(exp 5) at 1" and (is) approximately 10(exp 6) beyond 2" around 63 proposed members of nearby kinematic MGs. We review each of the kinematic associations to which our targets belong, concluding that five, beta Pictoris ((is) approximately 20 Myr), AB Doradus ((is) approximately 100 Myr), Columba ((is) approximately 30 Myr), Tucana-Horogium ((is) approximately 30 Myr), and TW Hydrae ((is) approximately 10 Myr), are sufficiently well-defined to constrain the ages of individual targets. Somewhat less than half of our targets are high-probability members of one of these MGs. For all of our targets, we combine proposed MG membership with other age indicators where available, including Ca ii HK emission, X-ray activity, and rotation period, to produce a posterior probability distribution of age. SEEDS observations discovered a substellar companion to one of our targets, kappa And, a late B star. We do not detect any other substellar companions, but do find seven new close binary systems, of which one still needs to be confirmed. A detailed analysis of the statistics of this sample, and of the companion mass constraints given our age probability distributions and exoplanet cooling models, will be presented in a forthcoming paper.

  12. Biochemical changes in hybrid pumpkin seeds at different stages of maturation

    Directory of Open Access Journals (Sweden)

    Patrícia Pereira da Silva

    Full Text Available ABSTRACT This study aimed to evaluate biochemical changes in seeds of the pumpkin hybrid, 'Jabras', from fruit harvested at different stages of maturation (15, 30, 45, 60 and 75 days after anthesis. Thirty fruit were harvested at each stage, with the seeds from 15 of the fruit being extracted immediately. The remaining 15 were stored for twenty days in plastic boxes and the seeds extracted after this period. After processing and drying the seeds, the following were determined: moisture content, germination, first count and antioxidant enzyme activity (peroxidase, ascorbate peroxidase, catalase and superoxide dismutase. Seeds from the fruit harvested at 30 DAA displayed low values for germination and vigour and high antioxidant enzyme activity, indicating that they were immature and that drying possibly caused damage to the system of cell membranes. The results obtained in this study demonstrated that analysis related to changes in the activity of enzymes during development and maturation of the seeds was effective in evaluating the physiological and biochemical changes in pumpkin seeds of the 'Jabras' cultivar.

  13. Quality control of system of imaging for rectal ultrasound for implants seed prostate low rate; Control de calidad del sistem de imagen por ecografia rectal para implantes de semillas de prostata de baja tasa

    Energy Technology Data Exchange (ETDEWEB)

    Luquero Llopis, N.; Ferrer Gracia, C.; Huertas Martinez, C.; Huerga Cabrerizo, C.; Corredoira Silva, E.; Serrada Hierro, A.

    2013-07-01

    In this work, the objective is the evaluation of the image system used in implants of prostate of low rate held at our hospital, for maximum control on the placement of the seeds in the patient and therefore carried out dosimetry. (Author)

  14. 基于高光谱图像处理的大豆品种识别%Identification of soybean seed varieties based on hyperspectral image

    Institute of Scientific and Technical Information of China (English)

    谭克竹; 柴玉华; 宋伟先; 曹晓达

    2014-01-01

    Different soybean seed varieties have different components (oil, protein, fat etc.) content. Identification of soybean seed varieties is a critical factor that improves the quality of produced soybean. In this study, hyperspectral image technique was applied in order to classify soybean seeds based on their varieties. The spectral reflectance data was collected using the optical sensor system with spectral region of 1000-2500nm. Principal component analysis (PCA) was performed to reduce the dimensionality of the data and remove the redundancy. Scores of four PCs were used as input features in the classification algorithm. Four texture feature parameters (angular second moment, energy, entropy and correlation) were extracted from each feature image selected by PCA. For the extraction of specific features, four significant feature parameters were computed from the 16 characteristic variables. Artificial neural network (ANN) classifier was employed for classification using top selected features. The obtained average training accuracy rate was 97.50%and the average testing accuracy rate was 93.88%. Thus, the results confirmed that hyperspectral image technique in-conjunction with BP neural network could be useful for soybean seed varieties classification.%大豆组分(油,蛋白质,脂肪等)在不同的大豆品种间差异很大。对于提高大豆品质来说,大豆品种识别是一个关键因素。该文利用高光谱图像技术对不同的大豆品种进行识别。利用高光谱成像系统获取大豆样本1000~2500 nm范围的光谱反射数据;应用主成分分析法(PCA,principal component analysis)对获取到的光谱数据进行数据降维并去除冗余数据;在分类算法中将得分高的主成分值作为输入特征,通过PCA方法从每个特征图像中提取4个特征变量(能量、熵、惯性矩和相关性);对于具体特征提取,从16个特征变量中提取8个重要特征参数;根据选择的

  15. Studies on the impact of fluoride toxicity on germination and seedling growth of gram seed (Cicer arietinum L. cv. Anuradha

    Directory of Open Access Journals (Sweden)

    Mondal N.K.

    2012-04-01

    Full Text Available The influence of 0, 0.1 mM, 0.5 mM, 1.0 mM, 4.0 mM, 8.0 mM fluoride (F concentration on seed germination, seedling growth of gram seeds (cv. Anuradha was studied under laboratory condition. At the end of 15 days of treatment, significant reduction in root length, shoot length, dry weight, fresh weight, % of germination, protein content, catalase activity, tolerance index, vigour index, germination rate, germination relative index, mean daily germination were observed at increasing fluoride concentration. Total soluble sugar content, proline content, peroxidase activity, ascorbic acid oxidase activity, % DFC, % phytotoxicity of root and shoot increased along with gradual increment of F concentration. 4.0 mM F concentration was found to be most sensitive for gram seeds. At 8.0 mM F concentration germination occurred but plants were totally dried after completion of treatment period.

  16. Control age - and irradiation-induced seed deterioration in lettuce (Lactuca sativa L.) by hydration-dehydration treatments

    International Nuclear Information System (INIS)

    Punjabi, Bina; Basu, R.N.

    1982-01-01

    Hydration-dehydration treatment of stored lettuce seed (1-year-old, medium-vigour), greatly slowed down their deterioration during subsequent storage under accelerated and natural ageing conditions. Hydration-dehydration of seeds, before or soon after X- and γ-irradiation, considerably minimized the adverse effect of irradiation on the development of biological after-effects responsible for the fall in germinibility, especially the large reduction of root growth of seedlings. Pre- and post-irradiation treatments gave broadly similar effects. The results have been discussed in terms of a possible involvement of a cellular (biochemical) repair mechanism in the hydration phase and also on the basis of a physico-chemical control of free radical pathology in the aged and irradiated seed. (author)

  17. Seed biopriming with novel strain of Trichoderma harzianum for the control of toxigenic Fusarium verticillioides and fumonisins in maize

    DEFF Research Database (Denmark)

    Nayaka, S.Chandra; Niranjana, S.R.; Shankar, A.C. Uday

    2010-01-01

    Fusarium verticillioides is one of the most important fungal pathogens in maize causing both pre- and post-harvest losses and also capable of producing Fumonisins. In the present study attempts have been made for screening potential T. harzianum from native rhizosphere and to study its effect...... on Fusarium ear rot disease, fumonisin accumulation in different maize cultivars grown in India. Eight isolates of T. harzianum were isolated and T. harzianum isolate Th-8 exhibited better antifungal activity than carbendizim. Th-8 was formulated in different solid substrates like wheat bran, paddy husk...... of 10 g/kg. Treated seeds were subjected to evaluate F. verticillioides incidence, seed germination, seedling vigour and field emergence, yield, thousand seed weight and fumonisin production. It was found that the pure culture of T. harzianum was more effective in reducing the F. verticillioides...

  18. Control age - and irradiation-induced seed deterioration in lettuce (Lactuca sativa L. ) by hydration-dehydration treatments

    Energy Technology Data Exchange (ETDEWEB)

    Punjabi, B; Basu, R N [University Coll. of Agriculture, Calcutta (India)

    1982-04-01

    Hydration-dehydration treatment of stored lettuce seed (1-year-old, medium-vigour), greatly slowed down their deterioration during subsequent storage under accelerated and natural ageing conditions. Hydration-dehydration of seeds, before or soon after X- and ..gamma..-irradiation, considerably minimized the adverse effect of irradiation on the development of biological after-effects responsible for the fall in germinibility, especially the large reduction of root growth of seedlings. Pre- and post-irradiation treatments gave broadly similar effects. The results have been discussed in terms of a possible involvement of a cellular (biochemical) repair mechanism in the hydration phase and also on the basis of a physico-chemical control of free radical pathology in the aged and irradiated seed.

  19. Role of relative humidity in processing and storage of seeds and assessment of variability in storage behaviour in Brassica spp. and Eruca sativa.

    Science.gov (United States)

    Suma, A; Sreenivasan, Kalyani; Singh, A K; Radhamani, J

    2013-01-01

    The role of relative humidity (RH) while processing and storing seeds of Brassica spp. and Eruca sativa was investigated by creating different levels of relative humidity, namely, 75%, 50%, 32%, and 11% using different saturated salt solutions and 1% RH using concentrated sulphuric acid. The variability in seed storage behaviour of different species of Brassica was also evaluated. The samples were stored at 40 ± 2°C in sealed containers and various physiological parameters were assessed at different intervals up to three months. The seed viability and seedling vigour parameters were considerably reduced in all accessions at high relative humidity irrespective of the species. Storage at intermediate relative humidities caused minimal decline in viability. All the accessions performed better at relative humidity level of 32% maintaining seed moisture content of 3%. On analyzing the variability in storage behaviour, B. rapa and B. juncea were better performers than B. napus and Eruca sativa.

  20. PHYSIOLOGICAL QUALITY OF Araucaria angustifolia (Bertol. Kuntze SEEDS SUBMITTED TO DIFFERENT STORAGE CONDITIONS AND ESCARIFICATION

    Directory of Open Access Journals (Sweden)

    Álvaro Valente Caçola

    2010-08-01

    Full Text Available The seeds of Araucaria angustifolia (Bertol. Kuntze are recalcitrants and, therefore, they lose rapidly the viability after the harvest, limiting their use for nursery production of seedlings. This work was carried out to investigate the effects of cold storage duration, conservation method, and escarification on germination and vigour of seeds of Araucaria angustifolia. The seeds were cold stored (0-1oC/90-95% RH for 0, 60, 120, and 180 days, under normal air condictions (NA, modified atmosphere (MA, and controlled atmosphere (CA storage. For each cold storage duration, after removal from cold storage, the seeds were submitted or not to scarification (by cutting a small portion of the apical seed tegument, avoiding the damage of the endosperm, letting the germinate in a chamber with controlled environment, in plastic trays filled with vermiculite, for 60 days. The experiment followed the completely randomized factorial design (4x3x2, with four cold storage durations (0, 60, 120, and 180 days, three storage methods (NA, MA, and CA, with or without escarification, and four replicates. There was a good preservation of physiological quality for seeds submitted to different conservation methods in cold storage along the 180-day period. However, there was a faster germination and initial growth of seedlings for seeds left in cold storage for 60 days than in seeds assessed at harvest or left in cold storage for 120-180 days. The NA storage of seeds in perfurated plastic bags was as efficient as MA and CA storage to preserve seeds quality. Seeds scarification increased vigor and promoted seedlings initial growth of Araucaria angustifolia after being removed from cold storage.

  1. Pesticidal seed coats based on azadirachtin-A: release kinetics, storage life and performance.

    Science.gov (United States)

    Nisar, Keyath; Kumar, Jitendra; Arun Kumar, M B; Walia, Suresh; Shakil, Najam A; Parsad, Rajender; Parmar, Balraj S

    2009-02-01

    Infestation of seeds by pests during storage leads to deterioration in quality. Seed coating is an effective option to overcome the menace. Unlike synthetic fungicidal seed coats, little is known of those based on botanicals. This study aims at developing azadirachtin-A-based pesticidal seed coats to maintain seed quality during storage. Polymer- and clay-based coats containing azadirachtin-A were prepared and evaluated for quality maintenance of soybean seed during storage. Gum acacia, gum tragacanth, rosin, ethyl cellulose, hydroxyethyl cellulose, polyethyl methacrylate, methyl cellulose, polyethylene glycol, polyvinyl chloride, polyvinyl acetate, polyvinyl pyrrolidone and Agrimer VA 6 polymers and the clay bentonite were used as carriers. The time for 50% release (t(1/2)) of azadirachtin-A into water from the seeds coated with the different coats ranged from 8.02 to 21.36 h. The half-life (T(1/2)) of azadirachtin-A in the coats on seed ranged from 4.37 to 11.22 months, as compared with 3.45 months in azadirachtin-A WP, showing an increase by a factor of nearly 1.3-3.3 over the latter. The coats apparently acted as a barrier to moisture to reduce azadirachtin-A degradation and prevented proliferation of storage fungi. Polyethyl methacrylate, polyvinyl acetate and polyvinyl pyrrolidone were significantly superior to the other polymers. Azadirachtin-A showed a significant positive correlation with seed germination and vigour, and negative correlation with moisture content. Effective polymeric carriers for seed coats based on azadirachtin-A are reported. These checked seed deterioration during storage by acting as a barrier to moisture and reduced the degradation of azadirachtin-A.

  2. Testes de vigor em sementes de Crambe abyssinica Vigor tests of Crambe abyssinica seeds

    Directory of Open Access Journals (Sweden)

    Luiz Antonio Silva dos Santos

    2013-02-01

    Full Text Available O objetivo deste trabalho foi verificar a eficiência de testes de vigor na avaliação da qualidade fisiológica de sementes de crambe. Para isso, quatro lotes de sementes de crambe da cultivar 'FMS Brilhante' foram submetidos aos testes de germinação e vigor (primeira contagem de germinação, comprimento e massa seca de plântula, emergência de plântulas em areia e campo, condutividade elétrica, envelhecimento acelerado e deterioração controlada, em delineamento inteiramente casualizado com quatro repetições. Os testes de emergência de plântulas em campo e de deterioração controlada com sementes pré umedecidas a 15% e mantidas a 42°C/48 horas e a 25% a 45°C/36 horas são eficientes na classificação dos lotes de sementes de crambe em distintos níveis de vigor.The objective was to verify vigor tests efficiency in the evaluation the crambe seeds physiological quality. Four lots of crambe seeds of cultivar 'FMS Brilhante' were submitted to the following standard germination and vigour test (first germination count, length and dry mass of seedling, seedling emergence in sand and field, electrical conductivity, accelerated ageing and controlled deterioration. The experiment was conducted in a randomized complete block design for test, evaluating the lots effect. The seedling emergence in field test and the controlled deterioration test at pre-moistened seeds at 15% and maintained under 42°C/48h and at 25% under 45°C/36h are efficients classifying the crambe seeds lots in different vigour levels.

  3. PHYSIOLOGICAL AND SANITARY QUALITY OF DESICCATED AND STORED AZUKI BEAN SEEDS

    Directory of Open Access Journals (Sweden)

    CÁSSIO JARDIM TAVARES

    2016-01-01

    Full Text Available The objective of this research was to evaluate the effects of using different herbicides as desiccants in pre - harvest and the effects of storage on the physiological and sanitary quality of azuki bean seeds ( Vigna angularis Willd. The experiment was arranged in a randomized complete block design in a split plot scheme, with four replications. Four herbicides were tested: paraquat (400 g a.i. ha - 1 , glufosinate ammonium (400 g a.i. ha - 1 , glyphosate (720 g a.i. ha - 1 , flumioxazin (30 g a.i. ha - 1 and a control without herbicide application. In the subplots seed quality was tested in two evaluation periods: at harvest and six months after harvest. Desiccant was applied when the azuki beans were physiologically mature. We assessed the physiological and sanitary quality of the seeds using a vigour and seed health test. The use of glyphosate resulted in a higher incidence of abnormal seedlings and reduced size and weight of the seedlings. With paraquat and flumioxazin the physiological quality was maintained and there was reduced pathogen infestation in the seeds six months after harvest. Storage affected the physiological quality of the azuki bean seeds.

  4. Effect of Ovule Position within the Pod on the Probability of Seed Production in Bauhinia ungulata (Fabaceae)

    Science.gov (United States)

    MENA-ALÍ, JORGE I.; ROCHA, OSCAR J.

    2004-01-01

    • Background and Aims It has been claimed that ovules linearly ordered within a fruit differ in their probabilities of reaching maturity. This was investigated by studying the effect the position of an ovule within the pod has on seed abortion and seed production in Bauhinia ungulata. • Methods Fruits collected during the dry seasons of 1999, 2000 and 2001 were opened, and the number, position and status of each ovule within the fruit were recorded. A GLM model was used to assess the effects of population, tree identity and ovule position within the pod on ovule fertilization, seed abortion, seed damage and seed maturation in two populations of B. ungulata. • Key Results Nearly 30 % of the ovules were not fertilized in 1999; this percentage dropped to 5 % the following two years. Seed abortion (50 %) and seed damage (15 %) were the same every year during the study period. Only 15 % of the initial ovules developed into mature seeds in 1999; this value increased to 35 % in 2000 and 2001. However, seed survivorship was dependent on the position of the ovule within the pod; non-fertilized and early aborted ovules were found more often near the basal end of the ovary. The frequency of seed damage was not affected by position. Mature seeds were found mainly in the stylar half of fruits, where ovules are likely to be fertilized by fast pollen tubes. • Conclusions The pattern of seed production in B. ungulata is non-random but is dependent upon the position of the ovule within the pod. The results suggest that the seeds produced within a fruit might differ in their vigour. PMID:15596452

  5. Recent approaches for bell pepper seed vigor testing.

    OpenAIRE

    DIAS, M. A. N.; MONDO, V. H. V.; CICERO, S. M.

    2014-01-01

    Seed vigor is a major attribute in the determination of whether a seed lot can be commercialized. Different physiological tests are used to assess seed vigor and are generally based on different concepts, which could lead to variable interpretations, particularly when comparing lots presenting different vigor levels. This study was conducted to compare the different approaches used to evaluate bell pepper seed vigor and verify the applicability of the Seed Vigor Imaging System (SVIS) for this...

  6. Recent approaches for bell pepper seed vigor testing

    OpenAIRE

    Dias, Marcos Altomani Neves; Mondo, Vitor Henrique Vaz; Cicero, Silvio Moure

    2014-01-01

    Seed vigor is a major attribute in the determination of whether a seed lot can be commercialized. Different physiological tests are used to assess seed vigor and are generally based on different concepts, which could lead to variable interpretations, particularly when comparing lots presenting different vigor levels. This study was conducted to compare the different approaches used to evaluate bell pepper seed vigor and verify the applicability of the Seed Vigor Imaging System (SVIS) for this...

  7. seed oil

    African Journals Online (AJOL)

    Wara

    Neem seed oil from the neem tree (Azadiracta indica) finds wide usage one of which is its utilization for cosmetics particularly .... obtained which is higher than that of olive oil 17. mgKOH/g (Davine ... The skin tolerance of shea fat employed as ...

  8. Priming of pioneer tree Guazuma ulmifolia (Malvaceae seeds evaluated by an automated computer image analysis Condicionamento fisiológico de sementes da árvore pioneira Guazuma ulmifolia (Malvaceae avaliado por análise computadorizada de imagens

    Directory of Open Access Journals (Sweden)

    Pedro Henrique Santin Brancalion

    2010-01-01

    Full Text Available Direct seeding is one of the most promising methods in restoration ecology, but low field seedling emergence from pioneer tree seeds still reduces its large scale applicability. The aim of this research was to evaluate seed priming for the pioneer tree species Guazuma ulmifolia. Priming treatments were selected based on seed hydration curves in water and in PEG 8000 solution. Seeds were primed in water for 16 h and in Polyethylene glycol - PEG 8000 (-0.8 MPa for 56 and 88 h at 20ºC to reach approximately 30% water content. Half of the seed sample of each treatment was dried back to the initial moisture content (7.2%; both dried and non-dried primed seeds as well as the unprimed seeds (control were tested for germination (percentage and rate and vigor (electrical conductivity of seed leachates. Seedling emergence percentage and rate were evaluated under greenhouse conditions, while seedling length and uniformity of seedling development were estimated using the automated image analysis software SVIS®. Primed seeds showed the highest physiological potential, which was mainly demonstrated by image analysis. Fresh or dried primed seeds in water for 16 h and in PEG (-0.8 MPa for 56 h, and fresh primed seeds in PEG for 88 h, improved G. ulmifolia germination performance. It is suggested that these treatments were promising to enhance efficiency of stand establishment of this species by direct seeding in restoration ecology programs.A semeadura direta é um dos métodos mais promissores para a restauração ecológica, mas a baixa emergência de plântulas em campo a partir de sementes de árvores pioneiras ainda limita sua aplicabilidade em larga escala. Avaliou-se a resposta de sementes da espécie florestal pioneira Guazuma ulmifolia ao condicionamento fisiológico. Os tratamentos foram selecionados com base em curvas de hidratação em água e em solução osmótica de Polietilenoglicol - PEG 8000. As sementes foram condicionadas em água por 16 h

  9. Improving tomato seed quality- challenges and possibilities

    DEFF Research Database (Denmark)

    Shrestha, Santosh

    The thesis investigates the possibility of using single seed near-infrared (NIR) spectroscopy, multispectral imaging (MSI) and NIR hyperspectral imaging (NIR-HSI) in combination with chemometrics for rapid determination of the tomato seed quality. The results of the PhD study are compiled in four...... manuscripts (MS). These non-destructive methods show the potential of sorting tomato seeds as per their viability and varietal identity. The results are discussed in the context of possible contribution from these methods in the improvement of the seed quality in Nepal. In MS I, potential application of NIR...... spectroscopy in combination with chemometrics for prediction of tomato seed viability is demonstrated. The work in MS I also emphasises on identifying the important NIR spectral regions for the chemometric model that are relevant to the separation of viable and non-viable seeds. The NIR-HIS method was also...

  10. Prediction of canned black bean texture (Phaseolus vulgaris L.) from intact dry seeds using visible/near infrared spectroscopy and hyperspectral imaging data.

    Science.gov (United States)

    Mendoza, Fernando A; Cichy, Karen A; Sprague, Christy; Goffnett, Amanda; Lu, Renfu; Kelly, James D

    2018-01-01

    Texture is a major quality parameter for the acceptability of canned whole beans. Prior knowledge of this quality trait before processing would be useful to guide variety development by bean breeders and optimize handling protocols by processors. The objective of this study was to evaluate and compare the predictive power of visible and near infrared reflectance spectroscopy (visible/NIRS, 400-2498 nm) and hyperspectral imaging (HYPERS, 400-1000 nm) techniques for predicting texture of canned black beans from intact dry seeds. Black beans were grown in Michigan (USA) over three field seasons. The samples exhibited phenotypic variability for canned bean texture due to genetic variability and processing practice. Spectral preprocessing methods (i.e. smoothing, first and second derivatives, continuous wavelet transform, and two-band ratios), coupled with a feature selection method, were tested for optimizing the prediction accuracy in both techniques based on partial least squares regression (PLSR) models. Visible/NIRS and HYPERS were effective in predicting texture of canned beans using intact dry seeds, as indicated by their correlation coefficients for prediction (R pred ) and standard errors of prediction (SEP). Visible/NIRS was superior (R pred = 0.546-0.923, SEP = 7.5-1.9 kg 100 g -1 ) to HYPERS (R pred = 0.401-0.883, SEP = 7.6-2.4 kg 100 g -1 ), which is likely due to the wider wavelength range collected in visible/NIRS. However, a significant improvement was reached in both techniques when the two-band ratios preprocessing method was applied to the data, reducing SEP by at least 10.4% and 16.2% for visible/NIRS and HYPERS, respectively. Moreover, results from using the combination of the three-season data sets based on the two-band ratios showed that visible/NIRS (R pred = 0.886, SEP = 4.0 kg 100 g -1 ) and HYPERS (R pred = 0.844, SEP = 4.6 kg 100 g -1 ) models were consistently successful in predicting texture over a wide range of measurements. Visible

  11. Artocarpus heterophyllus seeds inhibits sexual competence but not fertility of male rats.

    Science.gov (United States)

    Ratnasooriya, W D; Jayakody, J R A C

    2002-03-01

    According to Ayurvedic literature of Sri Lanka, roasted seeds of Artocarpus heterophyllus Lam. (Family: Moraceae) has aphrodisiac activity. However, some reproductively active young men in rural areas of Sri Lanka claim that consumption of these seeds few hours prior to coitus disrupts sexual function. Because of these two conflicting claims, it was thought useful to scientifically investigate the effects of A. heterophyllus seeds on male sexual function and fertility. This was done using a seed suspension in 1% methylcellulose (SS) in rats. In a sexual behaviour study using receptive female rats, an oral administration of 500 mg/kg dose of SS markedly inhibited libido, sexual arousal, sexual vigour and sexual performance within 2 hr. Further, the treatment induced a mild erectile dysfunction. These antimasculine effects on sexual function was not evident 6 hr post treatment indicating rapid onset and offset of action. Further, these actions on the sexual behaviour was not due to general toxicity, liver toxicity, stress or reduction in blood testosterone level but due to marked sedative activity. In a mating study, SS failed to alter ejaculating competence and fertility. These results suggest that A. heterophyllous seeds do not have aphrodisiac action, at least, in rats.

  12. Influence Mf Nitrogen and Phosphorus on Yield and Seed Quality of French Bean

    Directory of Open Access Journals (Sweden)

    SS Kakon

    2015-12-01

    Full Text Available The experiment was conducted at the research field of Agronomy Division, Bangladesh Agricultural Research Institute (BARI, Joydebpur, Gazipur during Rabi (winter season of 2010-11 to 2011-12 to determine the optimum rate of nitrogen and phosphorus fertilizer on yield and seed quality of French bean. A randomized complete block design was followed with ten treatment combinations such as N0P0, N0 P44, N50 P44, N100 P44, N150 P44, N200 P44, N150P0, N150P22, N150P33 and N150P55. Number of pods and yield of French bean were significantly increased with the increase in N (150 and P (44 kg ha--1. Averaged over the years, maximum number of pod (9.45 plant-1 and seed yield (1563.33 kg ha-1 were obtained when N and P were applied at the rate of 150 and 44 kg ha-1, respectively while lowest yield to N0P0 treatment in both the years. The treatment (N150P44 kg ha-1 gave the highest seed yield which was 51.4 and 54.30% higher than the control. Quality in terms of germination percentage and vigour index of harvested seed was also significantly influenced by higher doses of N and P while the lowest seed quality from plants that received no fertilizer in both the years.

  13. Weed seed predation in organic and conventional fields

    DEFF Research Database (Denmark)

    Navntoft, Søren; Wratten, S.D.; Kristensen, Kristian

    2009-01-01

    Enhanced biological control of weed seeds may improve sustainability of agricultural production. Biological control due to seed predation may be higher in organic fields because organic production generally supports more seed predators. To investigate such a difference, weed seed predation...... University and in two of the fields used for estimating seed predation. Recording of predators had therefore limited overlap with seed predation assays but was expected to give important information on key seed predators in the region. The mean seed removal rate was 17% in organic fields compared with 10...... edges. Overall, there was no consistent effect of distance from the field edge. Vegetation had a significant influence on the predation rates, with maximum rates at a medium-dense plant cover. Based on the video images, birds were the most important seed predators. The higher weed seed predation rate...

  14. High-Resolution Magic-Angle-Spinning NMR and Magnetic Resonance Imaging Spectroscopies Distinguish Metabolome and Structural Properties of Maize Seeds from Plants Treated with Different Fertilizers and Arbuscular mycorrhizal fungi.

    Science.gov (United States)

    Mazzei, Pierluigi; Cozzolino, Vincenza; Piccolo, Alessandro

    2018-03-21

    Both high-resolution magic-angle-spinning (HRMAS) and magnetic resonance imaging (MRI) NMR spectroscopies were applied here to identify the changes of metabolome, morphology, and structural properties induced in seeds (caryopses) of maize plants grown at field level under either mineral or compost fertilization in combination with the inoculation by arbuscular mycorrhizal fungi (AMF). The metabolome of intact caryopses was examined by HRMAS-NMR, while the morphological aspects, endosperm properties and seed water distribution were investigated by MRI. Principal component analysis (PCA) was applied to evaluate 1 H CPMG (Carr-Purcel-Meiboom-Gill) HRMAS spectra as well as several MRI-derived parameters ( T 1 , T 2 , and self-diffusion coefficients) of intact maize caryopses. PCA score-plots from spectral results indicated that both seeds metabolome and structural properties depended on the specific field treatment undergone by maize plants. Our findings show that a combination of multivariate statistical analyses with advanced and nondestructive NMR techniques, such as HRMAS and MRI, enables the evaluation of the effects induced on maize caryopses by different fertilization and management practices at field level. The spectroscopic approach adopted here may become useful for the objective appraisal of the quality of seeds produced under a sustainable agriculture.

  15. Chlorophyll in tomato seeds: marker for seed performance?

    NARCIS (Netherlands)

    Suhartanto, M.R.

    2002-01-01

    Using Xe-PAM, laser induced fluorometry and high performance liquid chromatography we found that chlorophyll was present in young tomato (cv. Moneymaker) seeds and was degraded during maturation. Fluorescence microscopy and imaging showed that the majority of chlorophyll is located in the

  16. Organic leek seed production - securing seed quality

    DEFF Research Database (Denmark)

    Deleuran, Lise Christina; Boelt, Birte

    2011-01-01

    To maintain integrity in organic farming, availability of organically produced GM-free seed of varieties adapted to organic production systems is of vital impor-tance. Despite recent achievements, organic seed supply for a number of vegetable species is insufficient. Still, in many countries...... seeds. Tunnel production is a means of securing seed of high genetic purity and quality, and organic leek (Allium porrum L.) seed production was tested in tunnels in Denmark. The present trial focused on steckling size and in all years large stecklings had a positive effect on both seed yield...

  17. Organic Leek Seed Production - Securing Seed Quality

    DEFF Research Database (Denmark)

    Deleuran, L C; Boelt, B

    2011-01-01

    To maintain integrity in organic farming, availability of organically produced GM-free seed of varieties adapted to organic production systems is of vital impor-tance. Despite recent achievements, organic seed supply for a number of vegetable species is insufficient. Still, in many countries...... seeds. Tunnel production is a means of securing seed of high genetic purity and quality, and organic leek (Allium porrum L.) seed production was tested in tunnels in Denmark. The present trial focused on steckling size and in all years large stecklings had a positive effect on both seed yield...

  18. In vitro seed germination of economically important edible bamboo Dendrocalamus membranaceus Munro.

    Science.gov (United States)

    Brar, Jasmine; Anand, Manju; Sood, Anil

    2013-01-01

    An in vitro propagation protocol using mature seeds of D. membranaceus was successfully established. Scarcity of seeds in bamboos because of their long flowering periods and irregular seed set resulting in low viability and germination potential, motivated us to undertake the present study. The effects of sterilants, light conditions, exogenous application of plant growth regulators and temperature in overcoming germination barriers in ageing seeds of bamboo were studied. It was found that HgCl2 (0.1%) along with bleach (15%) was more effective in raising aseptic cultures. Dark conditions, high temperatures around 30 degrees C and soaking of seeds in GA3 solution (50 ppm) overnight stimulated high percent of seed germination with corresponding increase in shoot length (2.7 +/- 0.7 mm) and number of sprouts (2.1 +/- 0.7) per explants during culture initiation. 6-benzylaminopurine acted synergistically with kinetin to give optimum germination rate of 70 +/- 13.9% as compared to 63.13% when used individually. For prolonged maintenance of cultures, 2% sucrose was found to be suitable for promoting photomixotrophic micropropagation. Following this procedure, about 65% survival of plantlets could be achieved during hardening. Biochemically seeds consume starchy endosperm for emergence of radicle which is taken as a sign of germination as also evident from the present study. Loss of viability and vigour after a year was confirmed by Tetrazolium chloride test. Micropropagation protocol developed here will ensure regeneration of large number of plants in a relatively short time. Conclusively, in vitro propagation protocol developed in D. membranaceus using mature seeds as an explants is reported for the first time.

  19. Influence of hydrogel on germination of lettuce and onion seed at different moisture levels

    Directory of Open Access Journals (Sweden)

    Kateřina Pazderů

    2013-01-01

    Full Text Available The influence of Agrisorb (water solution 1, 3, 5 g/l on lettuce and onion seed germination was tested in different moisture conditions (30 ml and 15 ml of water in germination box. Variants with reduced water level germinated much more slowly (MGT parameter than standard variants, though differences in total germination at the end of the test were insignificant. Treated variants of lettuce seeds showed a statistically significant increase in germination energy (GE on the first day (GE1, both water levels, but a significant decrease on the second day (columns GE2, 15 ml. Higher doses of Agrisorb slowed lettuce seed germination (GE2, 30 ml, dose 5 g significantly, similarly see GE2 (15 ml, doses 1, 3, 5 g. This slowdown was apparent for GE3 (both water amount as well. A similar but insignificant effect was evident for onions. There was an influence of cultivar and seed vigour on sensitivity to water stress. The hydrogel application influenced germination of lettuce and onion seeds. Treated lettuce seeds germinated faster than non-treated control in the beginning of germination process. This effect was not recorded in case of slowly germinated onion seed lots. Although influence of Agrisorb was positive in the beginning, higher doses of hydrogel reduced germination energy of treated seed lots (for example GE2, GE4 of both crops in comparison with non-treated control. Higher doses of hydrogel caused longer MGT of lettuce and onion as well.

  20. PHYSIOLOGICAL MATURATION IN SEEDS OF SWEET SOGHUM FOR FOLIAR FERTILISATION WITH SILICATE

    Directory of Open Access Journals (Sweden)

    BRUNO FRANÇA DA TRINDADE LESSA

    2017-01-01

    Full Text Available The aim of this study was to evaluate physiological quality in seeds of sweet sorghum grown under semi-arid conditions, and to determine the age of physiological maturity of the seeds as a function of the foliar application of potassium silicate. The experiment was carried out at the Curu Valley Experimental Farm, in Pentecoste in the state of Ceará, during the rainy seasons of 2014 and 2015. The BRS 506 and BRS 511 varieties were used, under foliar fertilisation with potassium silicate at doses of 500, 1000 and 1500 mL.ha -1, in addition to the control lots (with no application; harvesting was at four periods, 30, 37, 44 and 51 days after full bloom (DAB. The percentage and speed of germination were evaluated, together with the accelerated ageing test and seedling growth. The seeds presented greater than 90% germination from 37 DAB, reaching high seedling vigour at 51 DAB. Foliar fertilisation with potassium silicate under the conditions of the experiment resulted in an increase in the physiological quality of the seeds. The BRS 506 and BRS 511 cultivars displayed the highest physiological quality between 49 and 53 DAF.

  1. Seed shape quantification in the order Cucurbitales

    Directory of Open Access Journals (Sweden)

    Emilio Cervantes

    2018-02-01

    Full Text Available Seed shape quantification in diverse species of the families belonging to the order Cucurbitales is done based on the comparison of seed images with geometric figures. Quantification of seed shape is a useful tool in plant description for phenotypic characterization and taxonomic analysis. J index gives the percent of similarity of the image of a seed with a geometric figure and it is useful in taxonomy for the study of relationships between plant groups. Geometric figures used as models in the Cucurbitales are the ovoid, two ellipses with different x/y ratios and the outline of the Fibonacci spiral. The images of seeds have been compared with these figures and values of J index obtained. The results obtained for 29 species in the family Cucurbitaceae support a relationship between seed shape and species ecology. Simple seed shape, with images resembling simple geometric figures like the ovoid, ellipse or the Fibonacci spiral, may be a feature in the basal clades of taxonomic groups.

  2. Composição química e mobilização de reservas em sementes de soja de alto e baixo vigor Chemical composition and reserve mobilization in soybean seeds with high and low vigor

    Directory of Open Access Journals (Sweden)

    Fernando Augusto Henning

    2010-01-01

    Full Text Available O estudo dos efeitos do vigor das sementes sobre os estádios iniciais do desenvolvimento das plântulas de soja é importante, já que compreendem o período de estabelecimento da cultura. Sementes de baixo vigor podem provocar reduções na velocidade de emergência e na produção de biomassa seca de plântulas, podendo afetar o estabelecimento da cultura. Este estudo teve por objetivo quantificar os componentes químicos e avaliar a capacidade de mobilização de reservas, na germinação de sementes de soja de alto e baixo vigor. Foram utilizadas três cultivares: BRS 243RR, BRS 246RR e CD 219RR. A caracterização do vigor foi por meio do teste de envelhecimento acelerado. Para determinação da composição química das sementes, foram quantificados os teores de proteínas, amido e açúcar solúvel, além da atividade da isoenzima alfa amilase. A capacidade de mobilização das reservas na germinação foi avaliada por meio da massa seca e do comprimento das plântulas. De acordo com os resultados desse trabalho, em sementes mais vigorosas há maiores conteúdos de proteínas solúveis, amido e açúcares solúveis, bem como maior capacidade de mobilização de reservas na germinação, resultando em plântulas de soja com melhor desempenho inicial.The study of vigour effect on the initial stages of soybean seeds development is important, once it includes the period of crop establishment. Seeds with low vigour may result in reduction of emergence rate and dry matter production, which may affect the crop establishment. The aims of this study were to quantify chemical components and to evaluate the capacity of seed reserve mobilization in germination of soybean seeds with high and low vigour. Three cultivars, BRS 243RR, BRS 246RR and CD 219RR were studied. The characterization of seed vigour was performed by the accelerated aging test. To determine seed chemical composition, soluble sugar, starch and soluble protein were quantified; in

  3. A Classification Method for Seed Viability Assessment with Infrared Thermography

    Directory of Open Access Journals (Sweden)

    Sen Men

    2017-04-01

    Full Text Available This paper presents a viability assessment method for Pisum sativum L. seeds based on the infrared thermography technique. In this work, different artificial treatments were conducted to prepare seeds samples with different viability. Thermal images and visible images were recorded every five minutes during the standard five day germination test. After the test, the root length of each sample was measured, which can be used as the viability index of that seed. Each individual seed area in the visible images was segmented with an edge detection method, and the average temperature of the corresponding area in the infrared images was calculated as the representative temperature for this seed at that time. The temperature curve of each seed during germination was plotted. Thirteen characteristic parameters extracted from the temperature curve were analyzed to show the difference of the temperature fluctuations between the seeds samples with different viability. With above parameters, support vector machine (SVM was used to classify the seed samples into three categories: viable, aged and dead according to the root length, the classification accuracy rate was 95%. On this basis, with the temperature data of only the first three hours during the germination, another SVM model was proposed to classify the seed samples, and the accuracy rate was about 91.67%. From these experimental results, it can be seen that infrared thermography can be applied for the prediction of seed viability, based on the SVM algorithm.

  4. Surface coating for prevention of metallic seed migration in tissues

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Hyunseok; Park, Jong In [Program in Biomedical Radiation Sciences, Department of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 151-742 (Korea, Republic of); Lee, Won Seok; Park, Min [Interdisciplinary Program in Bioengineering, Seoul National University College of Engineering, Seoul 151-742 (Korea, Republic of); Son, Kwang-Jae [Hanaro Applications Research, Korea Atomic Energy Research Institute, Daejeon 305-353 (Korea, Republic of); Bang, Young-bong [Advanced Institutes of Convergence Technology, Seoul National University, Suwon 443-270 (Korea, Republic of); Choy, Young Bin, E-mail: ybchoy@snu.ac.kr, E-mail: sye@snu.ac.kr [Interdisciplinary Program in Bioengineering, Seoul National University College of Engineering, Seoul 110-744 (Korea, Republic of); Department of Biomedical Engineering, Seoul National University College of Medicine, Seoul 110-744 (Korea, Republic of); Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, Seoul 110-744 (Korea, Republic of); Ye, Sung-Joon, E-mail: ybchoy@snu.ac.kr, E-mail: sye@snu.ac.kr [Program in Biomedical Radiation Sciences, Department of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 151-742 (Korea, Republic of); Advanced Institutes of Convergence Technology, Seoul National University, Suwon 443-270 (Korea, Republic of); Department of Radiation Oncology, Seoul National University Hospital, Seoul 110-744 (Korea, Republic of)

    2015-06-15

    Purpose: In radiotherapy, metallic implants often detach from their deposited sites and migrate to other locations. This undesirable migration could cause inadequate dose coverage for permanent brachytherapy and difficulties in image-guided radiation delivery for patients. To prevent migration of implanted seeds, the authors propose a potential strategy to use a biocompatible and tissue-adhesive material called polydopamine. Methods: In this study, nonradioactive dummy seeds that have the same geometry and composition as commercial I-125 seeds were coated in polydopamine. Using scanning electron microscopy and x-ray photoelectron spectroscopy, the surface of the polydopamine-coated and noncoated seeds was characterized. The detachment stress between the two types of seeds and the tissue was measured. The efficacy of polydopamine-coated seed was investigated through in vitro migration tests by tracing the seed location after tissue implantation and shaking for given times. The cytotoxicity of the polydopamine coating was also evaluated. Results: The results of the coating characterization have shown that polydopamine was successfully coated on the surface of the seeds. In the adhesion test, the polydopamine-coated seeds had 2.1-fold greater detachment stress than noncoated seeds. From the in vitro test, it was determined that the polydopamine-coated seed migrated shorter distances than the noncoated seed. This difference was increased with a greater length of time after implantation. Conclusions: The authors suggest that polydopamine coating is an effective technique to prevent migration of implanted seeds, especially for permanent prostate brachytherapy.

  5. The estimation of some wild flowers seed material from the natural-valuable meadow habitats

    Directory of Open Access Journals (Sweden)

    Janicka Maria

    2016-12-01

    Full Text Available The restoration of the species composition of the meadow habitats is often linked to the introduction of the typical species’ seeds. The effectiveness of that treatment requires getting the acquired detailed knowledge of the germination biology of peculiar species. Eight typical plant species of four non-forest habitats of the river valleys, representing the following types: Cnidion dubii (6440, Molinion (6410, Arrhenatherion (6510 and Festuco-Brometea (6210 were investigated. The diasporas were collected in the years 2014–2015 on the meadows of PLH 140016 protection area near Mniszew (Kozienice county, Mazovian voivodeship. The ability of seeds’ germination in the laboratory conditions was studied. It was stated that Cnidion and Molinion meadows’ species require pre-chilling to break the seeds’ dormancy, while the Arrhenatherion meadows and xerotermophilous swards do not require such treatment. The Allium angulosum, Plantago lanceolata, Achillea millefolium and Eryngium planum were characterized by high vigour and germination capability as well as the low share of dead seeds. The extremely drought in 2015 caused the decreasing in germination capability and increasing in the dead seeds’ share. The preliminary studies, presented in this paper, show that Cnidion and Molinion meadows may be most difficult to restore because of the necessity of breaking the seeds’ dormancy and higher sensitiveness for the unfavourable weather conditions during the seed ripening stage. The studies have practical significance for the works connected with the floristic diversity’ increasing of threatened communities and the restoration of the destroyed meadow habitats.

  6. Physiological potential of Oryza sativa seeds treated with growth regulators at low temperatures

    Directory of Open Access Journals (Sweden)

    Mara Grohs

    Full Text Available ABSTRACT The rapid and uniform establishment of rice crops is important for improving production. However, this condition is influenced by several factors, including the soil temperature when planting, which may delay seed germination and compromise the final stand. The aim of this study was to evaluate the behaviour of substances which have the effect of growth regulator when applied to the seeds of different rice cultivars under low-temperature conditions. The experiment was carried out in a completely randomised design with four replications in a bi-factorial scheme, where factor A was represented by the different products (gibberellic acid - AG3, tiamethoxam - TMX, Haf Plus® - HAF, and a control with water - TEST, and factor B by the irrigated rice cultivars (IRGA 424, IRGA 425, Puitá INTA CL, and Avaxi CL. In addition, the experiment was repeated at temperatures of 17 °C and 25 °C in order to simulate low-temperature conditions. The results showed that AG3 is effective in increasing seed vigour in the rice cultivars at both temperatures, with the AG3, TMX and HAF responsible for increasing germination percentage only at the temperature of 17 °C. The effect of the products is more pronounced at low temperatures, and is dependent on the cultivar; in cultivars which are sensitive to cold there is no effect from the products used.

  7. What Are Chia Seeds?

    Science.gov (United States)

    ... your diet? Chia seeds come from the desert plant Salvia hispanica , a member of the mint family. ... ancient Aztec diet. The seeds of a related plant, Salvia columbariae (golden chia), were used primarily by ...

  8. Seeds and Synergies

    International Development Research Centre (IDRC) Digital Library (Canada)

    'Seeds and Synergies presents inspiring evidence of change in practice and policy ... Seeds of inspiration: breathing new life into the formal agricultural research .... and Urban Development and Poverty Alleviation and Agricultural Commodity ...

  9. Seeds as biosocial commons

    NARCIS (Netherlands)

    Patnaik, Archana

    2016-01-01

    This research investigates and describes the conservation and use of Plant Genetic Resources (PGRs), especially seeds through processes of commonisation. Seeds form an important element for sustaining human life (through food production) and social relations (by maintaining agricultural

  10. Seed dispersal in fens

    NARCIS (Netherlands)

    Middleton, Beth; van Diggelen, Rudy; Jensen, Kai

    Question: How does seed dispersal reduce fen isolation and contribute to biodiversity? Location: European and North American fens. Methods: This paper reviews the literature on seed dispersal to fens. Results: Landscape fragmentation may reduce dispersal opportunities thereby isolating fens and

  11. Demonstration of a forward iterative method to reconstruct brachytherapy seed configurations from x-ray projections

    Energy Technology Data Exchange (ETDEWEB)

    Murphy, Martin J; Todor, Dorin A [Department of Radiation Oncology, Virginia Commonwealth University, Richmond VA 23298 (United States)

    2005-06-07

    By monitoring brachytherapy seed placement and determining the actual configuration of the seeds in vivo, one can optimize the treatment plan during the process of implantation. Two or more radiographic images from different viewpoints can in principle allow one to reconstruct the configuration of implanted seeds uniquely. However, the reconstruction problem is complicated by several factors: (1) the seeds can overlap and cluster in the images; (2) the images can have distortion that varies with viewpoint when a C-arm fluoroscope is used; (3) there can be uncertainty in the imaging viewpoints; (4) the angular separation of the imaging viewpoints can be small owing to physical space constraints; (5) there can be inconsistency in the number of seeds detected in the images; and (6) the patient can move while being imaged. We propose and conceptually demonstrate a novel reconstruction method that handles all of these complications and uncertainties in a unified process. The method represents the three-dimensional seed and camera configurations as parametrized models that are adjusted iteratively to conform to the observed radiographic images. The morphed model seed configuration that best reproduces the appearance of the seeds in the radiographs is the best estimate of the actual seed configuration. All of the information needed to establish both the seed configuration and the camera model is derived from the seed images without resort to external calibration fixtures. Furthermore, by comparing overall image content rather than individual seed coordinates, the process avoids the need to establish correspondence between seed identities in the several images. The method has been shown to work robustly in simulation tests that simultaneously allow for unknown individual seed positions, uncertainties in the imaging viewpoints and variable image distortion.

  12. Recent approaches for bell pepper seed vigor testing

    Directory of Open Access Journals (Sweden)

    Marcos Altomani Neves Dias

    2014-08-01

    Full Text Available Seed vigor is a major attribute in the determination of whether a seed lot can be commercialized. Different physiological tests are used to assess seed vigor and are generally based on different concepts, which could lead to variable interpretations, particularly when comparing lots presenting different vigor levels. This study was conducted to compare the different approaches used to evaluate bell pepper seed vigor and verify the applicability of the Seed Vigor Imaging System (SVIS for this purpose. The experiment was performed using four seed lots of bell pepper hybrids in two periods, and the evaluations involved germination, seedling emergence, seedling emergence speed index, saturated salt accelerated aging and image analysis. The use of seedling imaging analysis by SVISis a new approach for bell pepper seed vigor evaluation. Both the software and traditional tests provided sufficient information for seed vigor analysis, enabling the segregation of the analyzed seed lots. Thus, the new method fulfills the requirement for an efficient, rapid, cost-effective and standardized method that allows comparisons among laboratories and avoids human influence on the interpretation of the results. SVIS can be considered a feasible option to be included in quality assurance programs of the bell pepper seed industry.

  13. Seed development and carbohydrates

    NARCIS (Netherlands)

    Wittich, P.E.

    1998-01-01

    Seeds assure the plant the onset of a next generation and a way of dispersal. They consist of endosperm and an embryo (originating from gametophytic tissue), enveloped by a seed coat (sporophytic tissue). Plants generate different types of seeds. For instance, the endosperm may either be

  14. Effect of biologically treated petroleum sludge on seed germination and seedling growth of Vigna unguiculata (L. Walp. (Fabaceae

    Directory of Open Access Journals (Sweden)

    Jeyabalan Sangeetha

    2014-06-01

    Full Text Available The present investigation was carried out to study the response of different concentrations of treated petroleum sludge on seed germination, root and shoot length and tolerance of Vigna unguiculata (L. Walp. The biologically treated petroleum sludge with bacterial consortium showed 54.8% reduction in total petroleum hydrocarbons. Treated sludge was utilized with agricultural soil in known concentration for the assessment of growth of V. unguiculata. A remarkable absence of seed germination was observed at higher sludge concentration. The different concentrations of treated petroleum sludge showed severe decline on the length, weight and vigour index of the tested seedlings with increasing sludge concentrations. The results showed that the difference in rate of seed germination was significant among various concentrations. Under environmental stress condition, germination is the most critical phase of life cycle in crop plants. In this present study, the high oil content found to alter the osmotic relation between seed and water and thus reduce the amount of water absorbed. It was concluded that the concentration of nutrients and oil present in the treated sludge were toxic to the plant.

  15. Silicon improves seed germination and alleviates drought stress in lentil crops by regulating osmolytes, hydrolytic enzymes and antioxidant defense system.

    Science.gov (United States)

    Biju, Sajitha; Fuentes, Sigfredo; Gupta, Dorin

    2017-10-01

    Silicon (Si) has been widely reported to have beneficial effect on mitigating drought stress in plants. However, the effect of Si on seed germination under drought conditions is still poorly understood. This research was carried out to ascertain the role of Si to abate polyethylene glycol-6000 mediated drought stress on seed germination and seedling growth of lentil. Results showed that drought stress significantly decreased the seed germination traits and increased the concentration of osmolytes (proline, glycine betaine and soluble sugars), reactive oxygen species (hydrogen peroxide and superoxide anion) and lipid peroxides in lentil seedlings. The activities of hydrolytic enzymes and antioxidant enzymes increased significantly under osmotic stress. The application of Si significantly enhanced the plants ability to withstand drought stress conditions through increased Si content, improved antioxidants, hydrolytic enzymes activity, decreased concentration of osmolytes and reactive oxygen species. Multivariate data analysis showed statistically significant correlations among the drought-tolerance traits, whereas cluster analysis categorised the genotypes into distinct groups based on their drought-tolerance levels and improvements in expression of traits due to Si application. Thus, these results showed that Si supplementation of lentil was effective in alleviating the detrimental effects of drought stress on seed germination and increased seedling vigour. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  16. A Reliable Methodology for Determining Seed Viability by Using Hyperspectral Data from Two Sides of Wheat Seeds.

    Science.gov (United States)

    Zhang, Tingting; Wei, Wensong; Zhao, Bin; Wang, Ranran; Li, Mingliu; Yang, Liming; Wang, Jianhua; Sun, Qun

    2018-03-08

    This study investigated the possibility of using visible and near-infrared (VIS/NIR) hyperspectral imaging techniques to discriminate viable and non-viable wheat seeds. Both sides of individual seeds were subjected to hyperspectral imaging (400-1000 nm) to acquire reflectance spectral data. Four spectral datasets, including the ventral groove side, reverse side, mean (the mean of two sides' spectra of every seed), and mixture datasets (two sides' spectra of every seed), were used to construct the models. Classification models, partial least squares discriminant analysis (PLS-DA), and support vector machines (SVM), coupled with some pre-processing methods and successive projections algorithm (SPA), were built for the identification of viable and non-viable seeds. Our results showed that the standard normal variate (SNV)-SPA-PLS-DA model had high classification accuracy for whole seeds (>85.2%) and for viable seeds (>89.5%), and that the prediction set was based on a mixed spectral dataset by only using 16 wavebands. After screening with this model, the final germination of the seed lot could be higher than 89.5%. Here, we develop a reliable methodology for predicting the viability of wheat seeds, showing that the VIS/NIR hyperspectral imaging is an accurate technique for the classification of viable and non-viable wheat seeds in a non-destructive manner.

  17. Priming effects on seed germination in Tecoma stans (Bignoniaceae) and Cordia megalantha (Boraginaceae), two tropical deciduous tree species

    Science.gov (United States)

    Alvarado-López, Sandra; Soriano, Diana; Velázquez, Noé; Orozco-Segovia, Alma; Gamboa-deBuen, Alicia

    2014-11-01

    Successful revegetation necessarily requires the establishment of a vegetation cover and one of the challenges for this is the scarce knowledge about germination and seedling establishment of wild tree species. Priming treatments (seed hydration during a specific time followed by seed dehydration) could be an alternative germination pre-treatment to improve plant establishment. Natural priming (via seed burial) promotes rapid and synchronous germination as well as the mobilisation of storage reserves; consequently, it increases seedling vigour. These metabolic and physiological responses are similar to those occurring as a result of the laboratory seed priming treatments (osmopriming and matrix priming) applied successfully to agricultural species. In order to know if natural priming had a positive effect on germination of tropical species we tested the effects of natural priming on imbibition kinetics, germination parameters (mean germination time, lag time and germination rate and percentage) and reserve mobilisation in the seeds of two tree species from a tropical deciduous forest in south-eastern México: Tecoma stans (L Juss. Ex Kunth) and Cordia megalantha (S.F Blake). The wood of both trees are useful for furniture and T. stans is a pioneer tree that promotes soil retention in disturbed areas. We also compared the effect of natural priming with that of laboratory matrix priming (both in soil). Matrix priming improved germination of both studied species. Natural priming promoted the mobilisation of proteins and increased the amount of free amino acids and of lipid degradation in T. stans but not in C. megalantha. Our results suggest that the application of priming via the burial of seeds is an easy and inexpensive technique that can improve seed germination and seedling establishment of tropical trees with potential use in reforestation and restoration practices.

  18. Tratamentos pré-germinativos em sementes de Arachis pintoi Arachis pintoi seeds as affect by pre-germination

    Directory of Open Access Journals (Sweden)

    Claudia Antonia Vieira Rossetto

    2008-02-01

    Full Text Available Neste Trabalho objetivou-se avaliar o efeito dos tratamentos prévios na germinação e no vigor de sementes de Arachis pintoi Krapov. & W. C. Greg.. O delineamento experimental adotado foi inteiramente casualizado, em esquema fatorial (2 lotes x 7 tratamentos, com quatro repetições. Para isto, foram utilizados dois lotes comerciais de sementes com o pericarpo (frutos de Arachis pintoi, da cv. Amarillo, que estavam armazenados por seis e 12 meses. Por lote, foram empregados os tratamentos de remoção ou não do pericarpo, de quebra do pericarpo, de exposição dos frutos íntegros ao aquecimento a 45º C por 48 e 72 horas e à hidratação por 24 e 48 horas. Posteriormente, por tratamento, foi realizada a avaliação do grau de umidade, da germinação e do vigor (primeira contagem e emergência de plântulas. A remoção do pericarpo tornou as sementes mais vulneráveis à ação dos microrganismos. O aquecimento a 45º C por 48 e 72 horas propiciou a redução das sementes não germinadas. A hidratação por 48 horas favoreceu a germinação e o vigor das sementes de Arachis pintoi.The aim was to evaluate the effects of previous treatments in the Arachis pintoi Krapov. & W. C. Greg. seeds germination and vigour. A completely randomized design with four replication arranged in a factorial scheme (2 lots x 7 treatments, was used. For this, two commercial lots of seeds with intact pods of Arachis pintoi, cv. Amarillo, stored by six and 12 months, were used. In each lot, the treatments were employed through pod removal or not, the pod breakage and the exposition of intact pods at 45ºC for 48 and 72 hours and to the hidratation for 24 and 48 hours. Further, for treatment, it was performed the evaluation of water content, germination and vigor (first count of germination and seedling emergency. The pod removal became the seeds vulnerable to the action of microorganism. Heating at 45ºC for 48 and 72 hours caused reduction of the nom

  19. Hot seeding using large Y-123 seeds

    International Nuclear Information System (INIS)

    Scruggs, S J; Putman, P T; Zhou, Y X; Fang, H; Salama, K

    2006-01-01

    There are several motivations for increasing the diameter of melt textured single domain discs. The maximum magnetic field produced by a trapped field magnet is proportional to the radius of the sample. Furthermore, the availability of trapped field magnets with large diameter could enable their use in applications that have traditionally been considered to require wound electromagnets, such as beam bending magnets for particle accelerators and electric propulsion. We have investigated the possibility of using large area epitaxial growth instead of the conventional point nucleation growth mechanism. This process involves the use of large Y123 seeds for the purpose of increasing the maximum achievable Y123 single domain size. The hot seeding technique using large Y-123 seeds was employed to seed Y-123 samples. Trapped field measurements indicate that single domain samples were indeed grown by this technique. Microstructural evaluation indicates that growth can be characterized by a rapid nucleation followed by the usual peritectic grain growth which occurs when large seeds are used. Critical temperature measurements show that no local T c suppression occurs in the vicinity of the seed. This work supports the suggestion of using an iterative method for increasing the size of Y-123 single domains that can be grown

  20. Oil palm seed distribution

    Directory of Open Access Journals (Sweden)

    Durand-Gasselin Tristan

    2005-03-01

    Full Text Available For a tropical plant, the oil palm commodity chain has the peculiarity of possessing a major seed production sector for reasons that are primarily genetic. This seed sector has numerous original aspects. Breeders are also propagators and usually also distribute their seeds. Oil palm seeds are semi-recalcitrant: they display pseudo-dormancy. Achieving seed germination is difficult and requires lengthy treatments and special installations. This restriction greatly influences seed distribution and the role of the different stakeholders in the commodity chain. It was only once it had been discovered how the “sh” gene functioned, which controls shell thickness, and when it became necessary to produce “tenera” seeds derived from exclusively “dura x pisifera” crosses, that a true seed market developed. In addition it is difficult to organize seed distribution to smallholders. This is partly due to difficulties that the profession, or a State-run organization, has in controlling middlemen networks, and partly to the absence of any protective systems (UPOV, plant breeder certificate, etc. that generally oblige breeders to preserve and propagate parents in their own installations. In fact there are major inequalities in the access to seeds between agroindustry and smallholders. Another peculiarity of the oil palm seed market is the virtually total absence of guarantees for buyers: the quality of the research conducted by breeders, the seed production strategies necessary for transferring genetic progress, and the technical quality of production. The only guarantee today comes from the relations of confidence established year after year between breeders/distributors and growers. In this fields, research can lead to some proposals: molecular biology offers some interesting prospects for certifying seed quality and social science develop effective communication methods.

  1. Does the informal seed system threaten cowpea seed health?

    NARCIS (Netherlands)

    Biemond, P.C.; Oguntade, O.; Lava Kumar, P.; Stomph, T.J.; Termorshuizen, A.J.; Struik, P.C.

    2013-01-01

    Most smallholder farmers in developing countries depend on an informal Seed System (SS) for their seed. The informal SS is often criticized because farmer-produced seed samples are not tested for seed health, thus accepting the risk of planting infected seeds. Here we aimed at assessing the quality

  2. Armazenamento de sementes de carolina em diferentes temperaturas e embalagens Storage of carolina seeds in different temperature and packing

    Directory of Open Access Journals (Sweden)

    Camila de Oliveira

    2012-01-01

    Full Text Available O objetivo do presente trabalho foi determinar a embalagem e a temperatura adequadas para o armazenamento de sementes de carolina. As sementes foram armazenadas em sacos de papel e de plástico, mantidas em 0±2; 10±2; 20±2°C e 60 5% de umidade relativa do ar (UR e em temperatura ambiente (23,4±3,3°C e 68,7±9%UR. O teor de água, a germinação e o vigor foram determinados trimestralmente. Durante o armazenamento, o teor de água das sementes foi de aproximadamente 8,9%. A embalagem saco de plástico e a temperatura de 0°C são adequadas para o armazenamento das sementes de carolina.The objective of the present research was to determine the more adequated packing and temperature for storage of "carolina" seeds. The seeds were stored in paper and plastic packings and kept in 0±2; 10±2; 20±2°C and 60 5% air relative humidity (ARH and in ambient temperature (23,4±3,3°C and 68,7±9% ARH. Quarterly, the water seed content, germinative test and seed vigour were avaluated. During storage, the seed water content was approximately 8.9%. The plastic packing (plastic bag and tempertature of 0oC are adequated for storage of "carolina" seeds.

  3. combining high seed number and weight to improve seed yield

    African Journals Online (AJOL)

    ACSS

    ABSTRACT. Increasing seed size and seed weight is an important trait for trade, yield component and adaptation of chickpea ... determining yield or quality, and the development of rapid and ..... C.G. 1981. Control of seed growth in soybeans.

  4. Producing the target seed: Seed collection, treatment, and storage

    Science.gov (United States)

    Robert P. Karrfalt

    2011-01-01

    The role of high quality seeds in producing target seedlings is reviewed. Basic seed handling and upgrading techniques are summarized. Current advances in seed science and technology as well as those on the horizon are discussed.

  5. Efficiency of alfalfa seed processing with different seed purity

    OpenAIRE

    Đokić, Dragoslav; Stanisavljević, Rade; Terzić, Dragan; Milenković, Jasmina; Radivojević, Gordana; Koprivica, Ranko; Štrbanović, Ratibor

    2015-01-01

    The work was carried out analysis of the impact of the initial purity of raw alfalfa seed on the resulting amount of processed seed in the processing. Alfalfa is very important perennial forage legume which is used for fodder and seed production. Alfalfa seed is possible to achieve high yields and very good financial effects. To obtain the seed material with good characteristics complex machines for cleaning and sorting seeds are used. In the processing center of the Institute for forage crop...

  6. Heterosis and combining ability of seed physiological quality traits of single cross vs. three-way sorghum hybrids

    Directory of Open Access Journals (Sweden)

    Marisol Galicia-Juárez

    2017-04-01

    Full Text Available The aim of this research was to compare genetic parameters for traits related with seed germination and seedling vigour of single cross (SCH vs. three-way (TWH sorghum hybrids. The study was conducted in a sand bed under a greenhouse; 67 genotypes (four pairs of A- and B- lines, four R- lines, 13 SCH and 42 TWH were allocated in a randomized complete blocks experimental design with three replications. Statistical analysis included ANOVA’s and Student “t” tests, while Tukey test (p £ 0.05 was applied for mean comparisons. The R-lines showed better physiological seed quality attributes than the A- and B- lines. The seed quality and heterosis values of SCH vs. that of TWH did not show significant differences (p > 0.05 for any variable; however, heterobeltiosis of SCH was higher than that of TWY for normal seedlings, dry weight of plumule and seedling emergence rate. The A2- line and R14 restorer line showed the highest GCA values in both types of hybrids. No differences (p ≤ 0.05 were found between maternal and paternal effects of the A- and B- lines involved in the male sterile cross (female parent of the TWH, except for plumule length in Line 5.

  7. Paper (essay on seed

    Directory of Open Access Journals (Sweden)

    Mirić Mladen

    2013-01-01

    Full Text Available Based on detailed studies of the past of the agrarian thought of the world (evolution of agro-innovation, and within it, the relationship of man and seed, the author has selected key data for Table 1. In addition, more or less known folk sayings, proverbs, curses and allegories in which the seed is the key word have been collected. Then, religious books, folk art and literature works (sayings of prominent individuals and experts and observations of the author himself have been studied. According to the collected opus, it can be concluded that the vast importance of seed meaningfully entwined into all spheres of folk life and cultural heritage. Seed is directly tied to the following eight key (revolutionary milestones in the food and economic life of mankind: 1. the first and for the longest time, the seed used to be the main food of the people and the first food reserve; 2. Neolithic Revolution was simply caused by the sowing of seeds; 3. for the sake of sowing man began to develop more complex processing techniques; 4. everywhere and forever, especially since the late 15th century, the seed has been a carrier of (rescuing plants between Europe and the New World, that is, between continents; 5. seed was the first product that has been chemically treated since the mid-18th century; 6. standard operation procedures and quality are promoted on seed by which it became the first product to have prescribed (compulsory methods, but it also became a good whose quality has to be tested before sale; 7. hybrid seed is a 'perpetrator' of the green revolution in the mid-20th century and at last there is disputable seed of genetically modified organisms that are spreading with certain reactions. The author proposes that the United Nations Standard International Trade Classification includes a special section (division which would classify the seed for sowing, while beyond this Classification terms such as seed, plant seed should be replaced with non-seed

  8. Seed dormancy and germination.

    Science.gov (United States)

    Penfield, Steven

    2017-09-11

    Reproduction is a critical time in plant life history. Therefore, genes affecting seed dormancy and germination are among those under strongest selection in natural plant populations. Germination terminates seed dispersal and thus influences the location and timing of plant growth. After seed shedding, germination can be prevented by a property known as seed dormancy. In practise, seeds are rarely either dormant or non-dormant, but seeds whose dormancy-inducing pathways are activated to higher levels will germinate in an ever-narrower range of environments. Thus, measurements of dormancy must always be accompanied by analysis of environmental contexts in which phenotypes or behaviours are described. At its simplest, dormancy can be imposed by the formation of a simple physical barrier around the seed through which gas exchange and the passage of water are prevented. Seeds featuring this so-called 'physical dormancy' often require either scarification or passage through an animal gut (replete with its associated digestive enzymes) to disrupt the barrier and permit germination. In other types of seeds with 'morphological dormancy' the embryo remains under-developed at maturity and a dormant phase exists as the embryo continues its growth post-shedding, eventually breaking through the surrounding tissues. By far, the majority of seeds exhibit 'physiological dormancy' - a quiescence program initiated by either the embryo or the surrounding endosperm tissues. Physiological dormancy uses germination-inhibiting hormones to prevent germination in the absence of the specific environmental triggers that promote germination. During and after germination, early seedling growth is supported by catabolism of stored reserves of protein, oil or starch accumulated during seed maturation. These reserves support cell expansion, chloroplast development and root growth until photoauxotrophic growth can be resumed. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

  9. Developing eco-friendly biofungicide for the management of major seed borne diseases of rice and assessing their physical stability and storage life.

    Science.gov (United States)

    Naveenkumar, Ramasamy; Muthukumar, Arjunan; Sangeetha, Ganesan; Mohanapriya, Ramanathan

    2017-04-01

    Three plant oils (Cymbopogon citratus, Cymbopogon martini, and Pelargonium graveolens) were developed as EC formulations and tested for their physical stabilities. EC formulations (10EC, 20EC and 30EC) of C. citratus, C. martini and P. graveolens had emulsion stability, spontaneity property, heat and cold stability. EC formulated plant oils were screened against the major seed borne fungi of rice such as Curvularia lunata, Fusarium moniliforme, Bipolaris oryzae, and Sarocladium oryzae. The level of inhibition varied among the concentrations of EC formulations. Among the three EC formulations, that of C. citratus oil 30EC recorded 100% inhibition on the mycelial growth of test pathogens. In the blotter paper method, rice seeds treated with a formulation of C. citratus oil 30EC controlled the infection of C. lunata, F. moniliforme, B. oryzae and S. oryzae in rice seed to the tune of 66.0%, 60.4%, 66.0% and 69.1%, respectively. Seed soaking with formulation of C. citratus oil 30EC showed the highest percentage of normal seedlings, the lowest number of abnormal seedling and fresh ungerminated seeds when tested with the roll-towel method. Seed soaking with 30EC formulation of C. citratus oil increased seed germination, shoot length, root length and vigour of rice seedlings when tested with the plastic tray method. Transmission of pathogens from seed to seedling was reduced significantly by the 30EC formulation of C. citratus oil when tested with the plastic pot method. The effect of the storage life of the 30EC formulation of C. citratus oil showed that it had retained their antifungal effect till the end of the incubation period (120 days), and is able to inhibit the mycelial growth of all test pathogens to the 100% level. Copyright © 2017 Académie des sciences. Published by Elsevier Masson SAS. All rights reserved.

  10. Artificial Seeds and their Applications

    Indian Academy of Sciences (India)

    currently working on ... heterozygosity of seed, minute seed size, presence of reduced ... Advantages of Artificial or Synthetic Seeds over Somatic Embryos for Propagation .... hour gives optimum bead hardness and rigidity for the produc-.

  11. Seed thioredoxin h

    DEFF Research Database (Denmark)

    Hägglund, Per; Finnie, Christine; Yano, Hiroyuki

    2016-01-01

    , for example chloroplastic f- and m-type thioredoxins involved in regulation of the Calvin-Benson cycle. The cytosolic h-type thioredoxins act as key regulators of seed germination and are recycled by NADPH-dependent thioredoxin reductase. The present review on thioredoxin h systems in plant seeds focuses...

  12. Saving Seed Microbiomes

    NARCIS (Netherlands)

    Berg, Gabriele; Raaijmakers, J.M.

    2018-01-01

    Plant seeds are home to diverse microbial communities whose composition is determined by plant genotype, environment, and management practices. Plant domestication is now recognized as an important driver of plant-associated microbial diversity. To what extent and how domestication affects seed

  13. Measurements and simulations of seeded electron microbunches with collective effects

    Directory of Open Access Journals (Sweden)

    K. Hacker

    2015-09-01

    Full Text Available Measurements of the longitudinal phase-space distributions of electron bunches seeded with an external laser were done in order to study the impact of collective effects on seeded microbunches in free-electron lasers. When the collective effects of Coulomb forces in a drift space and coherent synchrotron radiation in a chicane are considered, velocity bunching of a seeded microbunch appears to be a viable alternative to compression with a magnetic chicane under high-gain harmonic generation seeding conditions. Measurements of these effects on seeded electron microbunches were performed with a rf deflecting structure and a dipole magnet which streak out the electron bunch for single-shot images of the longitudinal phase-space distribution. Particle tracking simulations in 3D predicted the compression dynamics of the seeded microbunches with collective effects.

  14. Rapid analysis of seed size in Arabidopsis for mutant and QTL discovery

    Directory of Open Access Journals (Sweden)

    Baldwin Samantha

    2011-02-01

    Full Text Available Abstract Background Arabidopsis thaliana is a useful model organism for deciphering the genetic determinants of seed size; however the small size of its seeds makes measurements difficult. Bulk seed weights are often used as an indicator of average seed size, but details of individual seed is obscured. Analysis of seed images is possible but issues arise from variations in seed pigmentation and shadowing making analysis laborious. We therefore investigated the use of a consumer level scanner to facilitate seed size measurements in conjunction with open source image-processing software. Results By using the transmitted light from the slide scanning function of a flatbed scanner and particle analysis of the resulting images, we have developed a method for the rapid and high throughput analysis of seed size and seed size distribution. The technical variation due to the approach was negligible enabling us to identify aspects of maternal plant growth that contribute to biological variation in seed size. By controlling for these factors, differences in seed size caused by altered parental genome dosage and mutation were easily detected. The method has high reproducibility and sensitivity, such that a mutant with a 10% reduction in seed size was identified in a screen of endosperm-expressed genes. Our study also generated average seed size data for 91 Arabidopsis accessions and identified a number of quantitative trait loci from two recombinant inbred line populations, generated from Cape Verde Islands and Burren accessions crossed with Columbia. Conclusions This study describes a sensitive, high-throughput approach for measuring seed size and seed size distribution. The method provides a low cost and robust solution that can be easily implemented into the workflow of studies relating to various aspects of seed development.

  15. Glioblastoma with spinal seeding

    International Nuclear Information System (INIS)

    Fakhrai, N.; Fazeny-Doerner, B.; Marosi, C.; Czech, T.; Diekmann, K.; Birner, P.; Hainfellner, J.A.; Prayer, D.

    2004-01-01

    Background: extracranial seeding of glioblastoma multiforme (GBM) is very rare and its development depends on several factors. This case report describes two patients suffering from GBM with spinal seeding. In both cases, the anatomic localization of the primary tumor close to the cerebrospinal fluid (CSF) was the main factor for spinal seeding. Case reports: two patients with GBM and spinal seeding are presented. After diagnosis of spinal seeding, both patients were highly symptomatic from their spinal lesions. Case 1 experienced severe pain requiring opiates, and case 2 had paresis of lower limbs as well as urinary retention/incontinence. Both patients were treated with spinal radiation therapy. Nevertheless, they died 3 months after diagnosis of spinal seeding. Results: in both patients the diagnosis of spinal seeding was made at the time of cranial recurrence. Both tumors showed close contact to the CSF initially. Even though the patients underwent intensive treatment, it was not possible to keep them in a symptom-free state. Conclusion: because of short survival periods, patients deserve optimal pain management and dedicated palliative care. (orig.)

  16. Glioblastoma with spinal seeding

    Energy Technology Data Exchange (ETDEWEB)

    Fakhrai, N.; Fazeny-Doerner, B.; Marosi, C. [Clinical Div. of Oncology, Dept. of Medicine I, Univ. of Vienna (Austria); Czech, T. [Dept. of Neurosurgery, Univ. of Vienna (Austria); Diekmann, K. [Dept. of Radiooncology, Univ. of Vienna (Austria); Birner, P.; Hainfellner, J.A. [Clinical Inst. for Neurology, Univ. of Vienna (Austria); Prayer, D. [Dept. of Neuroradiology, Univ. of Vienna (Austria)

    2004-07-01

    Background: extracranial seeding of glioblastoma multiforme (GBM) is very rare and its development depends on several factors. This case report describes two patients suffering from GBM with spinal seeding. In both cases, the anatomic localization of the primary tumor close to the cerebrospinal fluid (CSF) was the main factor for spinal seeding. Case reports: two patients with GBM and spinal seeding are presented. After diagnosis of spinal seeding, both patients were highly symptomatic from their spinal lesions. Case 1 experienced severe pain requiring opiates, and case 2 had paresis of lower limbs as well as urinary retention/incontinence. Both patients were treated with spinal radiation therapy. Nevertheless, they died 3 months after diagnosis of spinal seeding. Results: in both patients the diagnosis of spinal seeding was made at the time of cranial recurrence. Both tumors showed close contact to the CSF initially. Even though the patients underwent intensive treatment, it was not possible to keep them in a symptom-free state. Conclusion: because of short survival periods, patients deserve optimal pain management and dedicated palliative care. (orig.)

  17. Physalis peruviana seed storage

    Directory of Open Access Journals (Sweden)

    Cíntia L. M. de Souza

    2016-03-01

    Full Text Available ABSTRACT Physalis peruviana belongs to Solanaceae family and has a high nutritional and nutraceutical potential. The production is intended for fruit consumption and the propagation is mainly by seeds. This study aimed to evaluate the influence of priming on the kinetics of germination of P. peruviana seeds stored at different temperatures. The seeds were stored at 5 and 25 °C in a chamber saturated with zinc chloride solution and in liquid nitrogen (-196 °C. Every 4 months, the seeds were removed from storage for evaluation of germination and moisture content in the laboratory and emergence and development of seedlings in greenhouse. During the last evaluation at 16 months, the seeds under the same conditions were subjected to salt stress. The moisture content varied during the storage period, but was always higher for seeds kept at -196 ºC. These seeds kept high germination percentage in water until 16 months, regardless of the tested temperature; however, in salt solution the germination percentage was significantly reduced.

  18. Effects of seed fermentation method on seed germination and vigor ...

    African Journals Online (AJOL)

    The present study was conducted to examine the influence of Lagenaria siceraria seed fermentation method on seed germination and vigor. Three seed fermentation methods (fermented in ambient air, plastic bag stored in ambient or in plastic bag buried) were tested on two cultivars during two years. Seed germination and ...

  19. Prosopis Africana SEEDS (OKPEYE)

    African Journals Online (AJOL)

    User

    Keywords: Prosopis africana, okpeye seeds, thermal heat conductivity, specific heat capacity, thermal heat diffusivity, .... 2.3 Determination of Thermal Properties of Prosopis. Africana .... and the guard ring was filled with fiber glass at both the.

  20. Oil seed marketing prospects

    International Nuclear Information System (INIS)

    Ceroni, G.

    1992-01-01

    With its 100 million tonnes annual production, the American continent is by far the world's biggest producer of oil seed, followed by Asia - 52 million, and Europe - 27 million tonnes. The Italian and European Communities have the farming capacity to double their production, but international agreements currently prohibit such initiatives. After first providing a panorama of the world oil seed market, this paper discusses new reforms in European Communities internal agricultural policies which currently limit production. These reforms, intended to encourage the production of oil seed for use as an ecological automotive fuel alternative, call for an obligatory set-aside of 15% of producing farm-land in exchange for the compensatory removal of oil seed production limits

  1. Genetics and Forest Seed Handling

    DEFF Research Database (Denmark)

    Schmidt, Lars Holger

    2016-01-01

    High genetic quality seed is obtained from seed sources that match the planting site, have a good outcrossing rate, and are superior in some desirable characters. Non-degraded natural forests and plantations may be used as untested seed sources, which can sometimes be managed to promote outbreeding...... and increase seed production. Planted seed orchards aim at capturing large genetic variation and are planted in a design that facilitates genetic evaluation and promotes outbred seed production. Good seed production relies upon success of the whole range of reproductive events from flower differentiation...

  2. Tumour seeding after percutaneous cryoablation for hepatocellular carcinoma

    Science.gov (United States)

    Wang, Chun-Ping; Wang, Hong; Qu, Jian-Hui; Lu, Yin-Ying; Bai, Wen-Lin; Dong, Zheng; Gao, Xu-Dong; Rong, Guang-Hua; Zeng, Zhen; Yang, Yong-Ping

    2012-01-01

    AIM: To assess the rate and risk factors for tumour seeding in a large cohort of patients. METHODS: Over an 8-year period, 1436 hepatocellular carcinoma (HCC) patients with 2423 tumour nodules underwent 3015 image-guided percutaneous cryoablation sessions [1215 guided by ultrasonography and 221 by spiral computed tomography (CT)]. Follow-up CT or magnetic resonance imaging was performed every 3 mo. The detailed clinical data were recorded to analyse the risk factors for seeding. RESULTS: The median follow-up time was 18 (range 1-90) mo. Seeding was detected in 11 patients (0.76%) at 1-24 (median 6.0) mo after cryoablation. Seeding occurred along the needle tract in 10 patients and at a distant location in 1 patient. Seeded tumours usually showed similar imaging and histopathological features to the primary HCCs. Univariate analyses identified subcapsular tumour location and direct subcapsular needle insertion as risk factors for seeding. Multivariate analysis showed that only direct subcapsular needle insertion was an independent risk factor for seeding (P = 0.017; odds ratio 2.57; 95%CI: 1.47-3.65). Seeding after cryoablation occurred earlier in patients with poorly differentiated HCC than those with well or moderately differentiated HCC [1.33 ± 0.577 mo vs 11.12 ± 6.896 mo; P = 0.042; 95%CI: (-19.115)-(-0.468)]. CONCLUSION: The risk of seeding after cryoablation for HCC is small. Direct puncture of subcapsular tumours should be avoided to minimise seeding. PMID:23236233

  3. Seeds of impurity

    Directory of Open Access Journals (Sweden)

    Andrea Pavoni

    2015-06-01

    Full Text Available Ai Weiwei’s art installation Kui Hua Zi [Sunflower Seeds] took place between 2010 and 2011 in the gigantic Turbine Hall of the Tate Modern Gallery, in London. It consisted of 100 millions hand-crafted porcelain seeds made in Jingdezhen, China.1 An uneven surface to dive into, a haptic space of undulating vision, rustling steps, unusual horizontality, a meaningless quicksand where the separation between artwork and spectator is engulfed, the immunity of distant contemplation denied.

  4. Storage of sunflower seeds

    Directory of Open Access Journals (Sweden)

    Denise de Castro Lima

    Full Text Available The sunflower is among the top five crops in the world for the production of edible vegetable oil. The species displays rustic behavior, with an excellent edaphic and climatic adaptability index, being able to be cultivated throughout Brazil. Seed quality is the key to increasing production and productivity in the sunflower. The objective of this work was to monitor the viability of sunflower seeds with a view to their conservation when stored in different environments and packaging. The seeds were packed in paper bags, multilayered paper, black polyethylene and PET bottles; and stored for a period of twelve months in the following environments: dry cold room (10 ºC and 55% RH, the ambient conditions of Fortaleza, Ceará, Brazil (30-32 ºC and 75% RH, refrigerator (4 ºC and 38-43% RH and freezer (-20 ºC. Every three months, the water content of the seeds was determined and germination, accelerated ageing, speed of emergence index, and seedling dry weight were evaluated. The experimental design was completely randomized, in a scheme of split-lots, with four replications. It can be concluded that the natural environment is not suitable for the storage of sunflower seeds. Sunflower seeds remain viable for 12 months when stored in a dry cold room, refrigerator or freezer, irrespective of the type of packaging used.

  5. Seed dispersal in fens

    Science.gov (United States)

    Middleton, B.; Van Diggelen, R.; Jensen, K.

    2006-01-01

    Question: How does seed dispersal reduce fen isolation and contribute to biodiversity? Location: European and North American fens. Methods: This paper reviews the literature on seed dispersal to fens. Results: Landscape fragmentation may reduce dispersal opportunities thereby isolating fens and reducing genetic exchange. Species in fragmented wetlands may have lower reproductive success, which can lead to biodiversity loss. While fens may have always been relatively isolated from each other, they have become increasingly fragmented in modern times within agricultural and urban landscapes in both Europe and North America. Dispersal by water, animals and wind has been hampered by changes related to development in landscapes surrounding fens. Because the seeds of certain species are long-lived in the seed bank, frequent episodes of dispersal are not always necessary to maintain the biodiversity of fens. However, of particular concern to restoration is that some dominant species, such as the tussock sedge Carex stricta, may not disperse readily between fens. Conclusions: Knowledge of seed dispersal can be used to maintain and restore the biodiversity of fens in fragmented landscapes. Given that development has fragmented landscapes and that this situation is not likely to change, the dispersal of seeds might be enhanced by moving hay or cattle from fens to damaged sites, or by reestablishing lost hydrological connections. ?? IAVS; Opulus Press.

  6. Seed-borne pathogens and electrical conductivity of soybean seeds

    Directory of Open Access Journals (Sweden)

    Adriana Luiza Wain-Tassi

    2012-02-01

    Full Text Available Adequate procedures to evaluate seed vigor are important. Regarding the electrical conductivity test (EC, the interference in the test results caused by seed-borne pathogens has not been clarified. This research was carried out to study the influence of Phomopsis sojae (Leh. and Colletotrichum dematium (Pers. ex Fr. Grove var. truncata (Schw. Arx. fungi on EC results. Soybean seeds (Glycine max L. were inoculated with those fungi using potato, agar and dextrose (PDA medium with manitol (-1.0 MPa and incubated for 20 h at 25 °C. The colony diameter, index of mycelial growth, seed water content, occurrence of seed-borne pathogens, physiological potential of the seeds, measured by germination and vigor tests (seed germination index, cold test, accelerated aging and electrical conductivity, and seedling field emergence were determined. The contents of K+, Ca2+, and Mg2+ in the seed and in the soaking solution were also determined. A complete 2 × 4 factorial design with two seed sizes (5.5 and 6.5 mm and four treatments (control, seeds incubated without fungi, seeds incubated with Phomopsis and seeds incubated with Colletotrichum were used with eight (5.5 mm large seeds and six (6.5 mm large seeds replications. All seeds submitted to PDA medium had their germination reduced in comparison to the control seeds. This reduction was also observed when seed vigor and leached ions were considered. The presence of Phomopsis sojae fungus in soybean seed samples submitted to the EC test may be the cause of misleading results.

  7. Optimal matching for prostate brachytherapy seed localization with dimension reduction.

    Science.gov (United States)

    Lee, Junghoon; Labat, Christian; Jain, Ameet K; Song, Danny Y; Burdette, Everette C; Fichtinger, Gabor; Prince, Jerry L

    2009-01-01

    In prostate brachytherapy, x-ray fluoroscopy has been used for intra-operative dosimetry to provide qualitative assessment of implant quality. More recent developments have made possible 3D localization of the implanted radioactive seeds. This is usually modeled as an assignment problem and solved by resolving the correspondence of seeds. It is, however, NP-hard, and the problem is even harder in practice due to the significant number of hidden seeds. In this paper, we propose an algorithm that can find an optimal solution from multiple projection images with hidden seeds. It solves an equivalent problem with reduced dimensional complexity, thus allowing us to find an optimal solution in polynomial time. Simulation results show the robustness of the algorithm. It was validated on 5 phantom and 18 patient datasets, successfully localizing the seeds with detection rate of > or = 97.6% and reconstruction error of < or = 1.2 mm. This is considered to be clinically excellent performance.

  8. The importance of using certified seed

    OpenAIRE

    Bogdanović Sandra; Mladenov Velimir; Balešević-Tubić Svetlana

    2015-01-01

    Certified seed is produced from the seed of known genetic origin and genetic purity with controlled and tested production, processed and declared in accordance with the Law on Seeds. Production of certified seed is carried out under the supervision of the Ministry of Agriculture and Environmental Protection, by seed producers formally listed in the Seed Register. Seed is processed in registered seed processing centres and quality is tested in laboratories accredited for seed testing. The orga...

  9. [Effects of exogenous salicylic acid on seed germination and physiological characteristics of Coronilla varia under drought stress.

    Science.gov (United States)

    Ma, Le Yuan; Chen, Nian Lai; Han, Guo Jun; Li, Liang

    2017-10-01

    This research investigated the effects of different concentrations (0, 0.5, 1.0, 2.0 mmol·L -1 ) of salicylic acid on the seed germination and physiological characteristics of legume forage Coronilla varia (cultivar 'Lvbaoshi') under PEG-6000 (concentration 8% and 12%) simulated drought stress. The results showed that under drought stress, 0.5-1.0 mmol·L -1 salicylic acid significantly increased germination percentage, germination vigour, germination index, vitality index and bud length of C. varia. Under the stress of 12% PEG, the dry mass of C. varia seedlings processed by 1.0 mmol·L -1 salicylic acid was significantly higher than that under drought stress. 0.5-1.0 mmol·L -1 salicylic acid processing significantly increased proline, soluble protein content, the activities of catalase, peroxidase and superoxide dismutase of C. varia seedlings under drought stress, but cell electrolyte permeability, H2O2 content and O2 - · production rate of seedlings were significantly decreased. 1.0 mmol·L -1 salicylic acid produced the best results. When the concentration of salicylic acid was beyond 2.0 mmol·L -1 , no mitigation effect was observed on the seed germination and growth of seedlings under drought stress. It was concluded that salicylic acid at appropriate concentrations could effectively improve osmotic regulation, antioxidation and mitigate the damage of drought stress so as to promote the growth of C. varia seedlings.

  10. Complementary method of analyzing the quality of the implant I-125 seeds for prostate brachytherapy using ultrasound imaging post-implant; Metodo complementario de analisis de la calidad del implante de semillas de I-125 para braquiterapia de prostata mediante la adquisicion de imagenes ecograficas post-implante

    Energy Technology Data Exchange (ETDEWEB)

    Jimenez Dominguez, M.; Carrasco Herrera, M.; Baeza Trujillo, M.; Herrador Cordoba, M.

    2011-07-01

    In this paper we propose a complementary method based on Longitudinal mode ultrasound images acquired the same day of surgery, at the end of the implant. This option will allow us to evaluate the dosimetry end of treatment with the patient in the same position he was planning and to the rectum and bladder just as full. This will permit the identification of bodies and the seeds of interest more easily and will have a reference with which to compare one month later, when the CT images can also detect whether there has been some migration.

  11. Magnetic-seeding filtration

    International Nuclear Information System (INIS)

    Ying, T.Y.; Chin, C.J.; Lu, S.C.; Yiacoumi, S.

    1997-10-01

    Magnetic-seeding filtration consists of two steps: heterogeneous particle flocculation of magnetic and nonmagnetic particles in a stirred tank and high-gradient magnetic filtration (HGMF). The effects of various parameters affecting magnetic-seeding filtration (HGMF). The effects of various parameters affecting magnetic seeding filtration are theoretically and experimentally investigated. A trajectory model that includes hydrodynamic resistance, van der Waals, and electrostatic forces is developed to calculate the flocculation frequency in a turbulent-shear regime. Fractal dimension is introduced to simulate the open structure of aggregates. A magnetic-filtration model that consists of trajectory analysis, a particle build-up model, a breakthrough model, and a bivariate population-balance model is developed to predict the breakthrough curve of magnetic-seeding filtration. A good agreement between modeling results and experimental data is obtained. The results show that the model developed in this study can be used to predict the performance of magnetic-seeding filtration without using empirical coefficients or fitting parameters. 35 refs., 7 figs., 1 tab

  12. Molecular physiology of seeds

    International Nuclear Information System (INIS)

    Hajduch, M.

    2014-05-01

    Plant development is well described. However, full understanding of the regulation of processes associated with plant development is still missing. Present Dr.Sc. thesis advances our understanding of the regulation of plant development by quantitative proteomics analyses of seed development of soybean, canola, castor, flax, and model plant arabidopsis in control and environmentally challenged environments. The analysis of greenhouse-grown soybean, canola, castor, and arabidospis provided complex characterization of metabolic processes during seed development, for instance, of carbon assimilation into fatty acids. Furthermore, the analyses of soybean and flax grown in Chernobyl area provided in-depth characterization of seed development in radio-contaminated environment. Soybean and flax were altered by radio-contaminated environment in different way. However, these alterations resulted into modifications in seed oil content. Further analyses showed that soybean and flax possess alterations of carbon metabolism in cytoplasm and plastids along with increased activity of photosynthetic apparatus. Our present experiments are focused on further characterization of molecular bases that might be responsible for alterations of seed oil content in Chernobyl grown plants. (author)

  13. 7 CFR 201.33 - Seed in bulk or large quantities; seed for cleaning or processing.

    Science.gov (United States)

    2010-01-01

    ... quantities; seed for cleaning or processing. (a) In the case of seed in bulk, the information required under... seeds. (b) Seed consigned to a seed cleaning or processing establishment, for cleaning or processing for... pertaining to such seed show that it is “Seed for processing,” or, if the seed is in containers and in...

  14. The effect of plant growth-promoting rhizobacteria on asparagus seedlings and germinating seeds subjected to water stress under greenhouse conditions.

    Science.gov (United States)

    Liddycoat, Scott M; Greenberg, Bruce M; Wolyn, David J

    2009-04-01

    Plant growth-promoting rhizobacteria (PGPR) can have positive effects on vigour and productivity, especially under stress conditions. In asparagus (Asparagus officinalis L.) field culture, seeds are planted in high-density nurseries, and 1-year-old crowns are transplanted to production fields. Performance can be negatively affected by water stress, transplant shock, and disease pressure on wounded roots. PGPR inoculation has the potential to alleviate some of the stresses incurred in the production system. In this study, the effects of PGPR (Pseudomonas spp.) treatment were determined on 3-week-old greenhouse-grown seedlings and germinating seeds of 2 asparagus cultivars. The pots were irrigated to a predetermined level that resulted in optimum growth or the plants were subjected to drought or flooding stress for 8 weeks. The cultivars responded differently to PGPR: single inoculations of seedlings enhanced growth of 'Guelph Millennium' under optimum conditions and 'Jersey Giant' seedlings under drought stress. Seed inoculations with PGPR resulted in a positive response only for 'Guelph Millennium', for which both single or multiple inoculations enhanced plant growth under drought stress.

  15. Pancreatic cancer seeding of percutaneous needle tract

    Directory of Open Access Journals (Sweden)

    Qiao Zhou, MD

    2017-03-01

    Full Text Available A 65-year old African-American female presents with biliary ductal dilatation due to an obstructive pancreatic head mass. Percutaneous transhepatic cholangiogram performed and biliary drainage catheter placement for decompression of the biliary system. The patient had a Whipple procedure performed several months later. On follow up CT imaging, there was interval development and enlargement of a subcutaneous lesion by the right oblique muscles. Biopsy of this lesion revealed pancreatic adenocarcinoma from percutaneous seeding of the transhepatic needle tract.

  16. Characterization of seeds with different moisture content by photoacoustic microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Dominguez Pacheco, Arturo; Hernandez Aguilar, Claudia; Marinez Ortiz, Efrain [Instituto Politecnico Nacional, Sepi-Esime, Zacatenco. Unidad Profesional ' Adolfo Lopez Mateos' . Col. Lindavista. Mexico D.F., CP 07738 (Mexico); Cruz-Orea, Alfredo; Ayala-Maycotte, Esther, E-mail: fartur@hotmail.co [Departamento de Fisica, CINVESTAV - IPN, A. P. 14-740, Mexico D.F., C.P. 07360 (Mexico)

    2010-03-01

    Photoacoustic (PA) technique has important applications for material characterization and nondestructive evaluation of opaque solid materials. PA microscopy allows the acquisition of information of samples with inhomogeneous structures as agricultural seeds. A determining factor for seed safe storage is their moisture content. Seeds stored at high moisture content exhibit increased respiration, heating, and fungal invasion resulting in poor seed vigor and viability. Low moisture content, in the seed to be stored, is the best prevention for these problems. In this study, Photoacoustic Microscopy (PAM) was used to characterize seeds with different moisture content. In the PAM experimental setup the photoacoustic cell and its sensor, an electret microphone, are mounted on an x-y stage of mobile axes, with spatial resolution of 70 {mu}m. The excitation light source is a fiber coupled laser diode, at 650 nm wavelength, modulated in intensity at 1 Hz of frequency, by the reference oscillator of a lock-in amplifier. By using a microscope objective the laser beam was focused on the seed surface. The resolution was enough to obtain differences in the obtained images, which are dependent on the moisture content. This method, to study differences in the seed moisture content, is nondestructive and could be useful for a sustainable Agriculture.

  17. The Effect of Seed Priming and Transplanting on Morphological Characteristics, Yield and Yield Components of SuperSweet Corn

    Directory of Open Access Journals (Sweden)

    matin haghighi khah

    2017-08-01

    Full Text Available Introduction Corn (Zea mays L. is the third most important cereal crop in the world after wheat and rice (Lashkari et al., 2011. Corn production has been extended in to the whole world during the course of the last century due to its compatibility. It has more diversity compare to other cereal. Many corn types are cultivated, including field corn, ornamental corn, popcorn, sweet corn and several different supersweet corns. Sweet corn, considered a vegetable, is a special type of corn with particular characteristics, such as sweet taste, thin pericarp and endosperm with delicate texture, and high nutritional value. It is destined exclusively for human consumption, in fresh form or in processed foods, whereas the straw can be used for silage after harvest (Santos et al., 2014. Sweet corn seeds germinate slowly and exhibit poor seedling vigour. Poor germination in sweet corn has been attributed to low seed vigour and susceptibility to seed and soilborne diseases (Ratin et al., 2006. Seed priming is the one of efficient method to improve germination and emergence. In addition, transplanting provides optimal environmental conditions for seed germination and avoids planting seeds in disease-contaminated soil (Khalid et al., 2012. Materials and methods To investigate the effect of seed priming and transplanting on morphological characteristics, yield and yield components of supersweet corn a series of greenhouse and field experiments were conducted in a factorial based design on a randomized complete block in 2013. This experiment was conducted in the greenhouse to determine the best seed priming treatments. The treatments were hydro priming, Poly ethylene glycol (6000 -0.4 and -0.8 MPa, Sodium Sulphate 0.1 and 0.5%, Zinc Sulphate 1 and 0.5%, Copper Sulphate 0.1 and 0.5% and Control for 36 hours. After that the seeds washed by distillated water and dried back in laboratory conditions. Then treated seeds were sown in trays that contained by cocopeatand

  18. Improving Soil Seed Bank Management.

    Science.gov (United States)

    Haring, Steven C; Flessner, Michael L

    2018-05-08

    Problems associated with simplified weed management motivate efforts for diversification. Integrated weed management uses fundamentals of weed biology and applied ecology to provide a framework for diversified weed management programs; the soil seed bank comprises a necessary part of this framework. By targeting seeds, growers can inhibit the propagule pressure on which annual weeds depend for agricultural invasion. Some current management practices affect weed seed banks, such as crop rotation and tillage, but these tools are often used without specific intention to manage weed seeds. Difficulties quantifying the weed seed bank, understanding seed bank phenology, and linking seed banks to emerged weed communities challenge existing soil seed bank management practices. Improved seed bank quantification methods could include DNA profiling of the soil seed bank, mark and recapture, or 3D LIDAR mapping. Successful and sustainable soil seed bank management must constrain functionally diverse and changing weed communities. Harvest weed seed controls represent a step forward, but over-reliance on this singular technique could make it short-lived. Researchers must explore tools inspired by other pest management disciplines, such as gene drives or habitat modification for predatory organisms. Future weed seed bank management will combine multiple complementary practices that enhance diverse agroecosystems. This article is protected by copyright. All rights reserved.

  19. Seeds of confusion : the impact of policies on seed systems

    OpenAIRE

    Louwaars, N.P.

    2007-01-01

    Seed is basic to crop production. Next to its importance in production, food security and rural development, seed is a key element in many debates about technology development and transfer, biodiversity, globalisation and equity. The sustainable availability of good quality seed is thus an important development issue. This study deals with the impact different types of regulation have on how farmers access seed. I have analysed current regulatory frameworks in terms of their impact on differe...

  20. Magnetic-seeding filtration

    International Nuclear Information System (INIS)

    Depaoli, D.

    1996-01-01

    This task will investigate the capabilities of magnetic-seeding filtration for the enhanced removal of magnetic and nonmagnetic particulates from liquids. This technology appies to a wide range of liquid wastes, including groundwater, process waters, and tank supernatant. Magnetic-seeding filtration can be used in several aspects of treatment, such as (1) removal of solids, particularly those in the colloidal-size range that are difficult to remove by conventional means; (2) removal of contaminants by precipitation processes; and (3) removal of contaminants by sorption processes

  1. genetics and inheritance of seed dormancy inflicted by seed

    African Journals Online (AJOL)

    Mgina

    ABSTRACT. The study was undertaken to investigate the genetic mode of inheritance of dormancy imposed by the hull (seed coat) in rice seeds. Freshly harvested seeds of parents, F1 and F2 populations of a cross between a dormant cultivar Kisegese and non-dormant strain K2004 were used. Germination test of the ...

  2. Seeds of confusion : the impact of policies on seed systems

    NARCIS (Netherlands)

    Louwaars, N.P.

    2007-01-01

    Seed is basic to crop production. Next to its importance in production, food security and rural development, seed is a key element in many debates about technology development and transfer, biodiversity, globalisation and equity. The sustainable availability of good quality seed is thus an important

  3. Effects of seed collecting date and storage duration on seed ...

    African Journals Online (AJOL)

    The objective of this study was to determine the effect of seed collecting dates (5 to 6 times from mid-November to early January, 10 days intervals) and seed storage duration (4, 8, and 12 months) at room temperature on seed germination of four Artemisia species (Artemisia sieberi, A. diffusa, A. kupetdaghensis, and A.

  4. Cone and seed yields in white spruce seed production areas

    Science.gov (United States)

    John A. Pitcher

    1966-01-01

    The source of seed is an important consideration in the reforestation program on the National Forests in the North Central Region. Thirty-five seed production areas have been set up in the Region, along the lines proposed by the North Central Forest Experiment Station, to provide control of seed source. Red pine, white pine, shortleaf and loblolly pine, and white...

  5. seed germination and seedlings growth

    African Journals Online (AJOL)

    STORAGESEVER

    2007-12-17

    Dec 17, 2007 ... The role of 20E in plant physiology including seed germination is not studied. ..... GA3, ABA and CKs on lettuce Lactuca sativa seed germination are ..... Practical uses for ecdysteroids in mammals and humans: an update. J.

  6. Seed research for improved technologies

    OpenAIRE

    Bino,R.J.; Jalink,H.; Oluoch,M.O.; Groot,S.P.C.

    1998-01-01

    The production of high-quality seed is the basis for a durable a profitable agriculture. After production, seed is processed, conditioned, stored, shipped and germinated. For quality assurance, seed quality has to be controlled at all steps of the production chain. Seed functioning is accompanied by programmed transitions from cell proliferation to quiescence upon maturation and from quiescence to reinitiation of cellular metabolism upon imbibition. Despite the obvious importance of these con...

  7. (Lupinus albus) SEEDS

    African Journals Online (AJOL)

    user

    2010-08-08

    Aug 8, 2010 ... lupin samples indicated that lupins can be used as a raw material for various food ... lupin seeds can be utilized for milk and meat imitation products. ... estimated by multiplying the percentage of crude protein, crude fat and ...

  8. Seed for change

    NARCIS (Netherlands)

    Hassena Beko, Mohammed

    2017-01-01

    Ethiopia is an agrarian country where agriculture dominates the economy, and thus agriculture is considered as an engine of growth by the government. Seed as one of the agricultural technologies, in fact, a carrier of many technologies, is critical to increasing production, but the use of quality

  9. Managing Stress. Project Seed.

    Science.gov (United States)

    Muto, Donna; Wilk, Jan

    One of eight papers from Project Seed, this paper describes a stress management project undertaken with high school sophomores. Managing Stress is described as an interactive workshop that offers young people an opportunity to examine specific areas of stress in their lives and to learn effective ways to deal with them. The program described…

  10. Grape Seed Extract

    Science.gov (United States)

    ... Greece people have used grapes, grape leaves, and sap for health purposes. Grape seed extract was developed ... sharing research results, and educating the public. Its resources include publications (such as Dietary ... Department of Health & Human Services, National Institutes of Health, National Center for ...

  11. The SEED Initiative

    Science.gov (United States)

    Teich, Carolyn R.

    2011-01-01

    Committed to fulfilling the promise of the green economy, the American Association of Community Colleges (AACC) launched the Sustainability Education and Economic Development (SEED) initiative (www.theseedcenter.org) in October 2010. The project advances sustainability and clean energy workforce development practices at community colleges by…

  12. Dormancy in Plant Seeds

    NARCIS (Netherlands)

    Hilhorst, H.W.M.; Finch-Savage, W.E.; Buitink, J.; Bolingue, W.; Leubner-Metzger, G.

    2010-01-01

    Seed dormancy has been studied intensely over the past decades and, at present, knowledge of this plant trait is at the forefront of plant biology. The main model species is Arabidopsis thaliana, an annual weed, possessing nondeep physiological dormancy. This overview presents the state-of-the-art

  13. Magnetic-seeding filtration

    Energy Technology Data Exchange (ETDEWEB)

    DePaoli, D.W.; Tsouris, C. [Oak Ridge National Lab., TN (United States); Yiacoumi, Sotira

    1997-10-01

    Magnetic-seeding filtration is a technology under development for the enhanced removal of magnetic and non-magnetic particulates from liquids. This process involves the addition of a small amount of magnetic seed particles (such as naturally occurring iron oxide) to a waste suspension, followed by treatment with a magnetic filter. Non-magnetic and weakly magnetic particles are made to undergo nonhomogeneous flocculation with the seed particles, forming flocs of high magnetic susceptibility that are readily removed by a conventional high-gradient magnetic filter. This technology is applicable to a wide range of liquid wastes, including groundwater, process waters, and tank supernatants. Magnetic-seeding filtration may be used in several aspects of treatment, such as (1) removal of solids, particularly those in the colloidal size range that are difficult to remove by conventional means; (2) removal of contaminants by precipitation processes; and (3) removal of contaminants by sorption processes. Waste stream characteristics for which the technology may be applicable include (1) particle sizes ranging from relatively coarse (several microns) to colloidal particles, (2) high or low radiation levels, (3) broad-ranging flow rates, (4) low to moderate solids concentration, (5) cases requiring high decontamination factors, and (6) aqueous or non-aqueous liquids. At this point, the technology is at the bench-scale stage of development; laboratory studies and fundamental modeling are currently being employed to determine the capabilities of the process.

  14. Magnetic-seeding filtration

    International Nuclear Information System (INIS)

    DePaoli, D.W.; Tsouris, C.; Yiacoumi, Sotira.

    1997-01-01

    Magnetic-seeding filtration is a technology under development for the enhanced removal of magnetic and non-magnetic particulates from liquids. This process involves the addition of a small amount of magnetic seed particles (such as naturally occurring iron oxide) to a waste suspension, followed by treatment with a magnetic filter. Non-magnetic and weakly magnetic particles are made to undergo nonhomogeneous flocculation with the seed particles, forming flocs of high magnetic susceptibility that are readily removed by a conventional high-gradient magnetic filter. This technology is applicable to a wide range of liquid wastes, including groundwater, process waters, and tank supernatants. Magnetic-seeding filtration may be used in several aspects of treatment, such as (1) removal of solids, particularly those in the colloidal size range that are difficult to remove by conventional means; (2) removal of contaminants by precipitation processes; and (3) removal of contaminants by sorption processes. Waste stream characteristics for which the technology may be applicable include (1) particle sizes ranging from relatively coarse (several microns) to colloidal particles, (2) high or low radiation levels, (3) broad-ranging flow rates, (4) low to moderate solids concentration, (5) cases requiring high decontamination factors, and (6) aqueous or non-aqueous liquids. At this point, the technology is at the bench-scale stage of development; laboratory studies and fundamental modeling are currently being employed to determine the capabilities of the process

  15. [Study on seed testing for Salvia miltiorrhiza].

    Science.gov (United States)

    Dan, Hong-mei; Qi, Jian-jun; Zhou, Li-li; Li, Xian-en

    2008-09-01

    To establish a seed testing methods for Salvia miltiorrhiza. Referring to the International Seed Testing Rules made by ISTA and the Seed Testing for Crops (GB/T3543. 1-1995) issued by China. The seeds are selected by winnowing; the seed purity is about 50%-60%; 100 grain weight is used to determine the quality of the seed; the seed moisture content is determined by air drying, the drying hour is 3 h. Seed viability is tested by TFC method.

  16. Germination of red alder seed.

    Science.gov (United States)

    M.A. Radwan; D.S. DeBell

    1981-01-01

    Red alder seeds were collected from six locations throughout the natural range of the species. Each seed lot was obtained from a single tree, and the seeds were used to determine germination with and without stratification treatment. Irrespective of treatment, germination varied significantly (P

  17. Nest-mediated seed dispersal

    Science.gov (United States)

    Robert J. Warren; Jason P. Love; Mark A. Bradford

    2017-01-01

    Many plant seeds travel on the wind and through animal ingestion or adhesion; however, an overlooked dispersal mode may lurk within those dispersal modes. Viable seeds may remain attached or embedded within materials birds gather for nest building. Our objective was to determine if birds inadvertently transport seeds when they forage for plant materials to...

  18. Seed systems support in Kenya

    NARCIS (Netherlands)

    Munyi, Peter; Jonge, De Bram

    2015-01-01

    The threats of climate change and rising food prices have stirred renewed attention for seed and food security in Africa, inviting new thinking on the role of seed sector development in coping with these concerns. One conceptual framework that has gained attention is the Integrated Seed Sector

  19. Automated seeding-based nuclei segmentation in nonlinear optical microscopy.

    Science.gov (United States)

    Medyukhina, Anna; Meyer, Tobias; Heuke, Sandro; Vogler, Nadine; Dietzek, Benjamin; Popp, Jürgen

    2013-10-01

    Nonlinear optical (NLO) microscopy based, e.g., on coherent anti-Stokes Raman scattering (CARS) or two-photon-excited fluorescence (TPEF) is a fast label-free imaging technique, with a great potential for biomedical applications. However, NLO microscopy as a diagnostic tool is still in its infancy; there is a lack of robust and durable nuclei segmentation methods capable of accurate image processing in cases of variable image contrast, nuclear density, and type of investigated tissue. Nonetheless, such algorithms specifically adapted to NLO microscopy present one prerequisite for the technology to be routinely used, e.g., in pathology or intraoperatively for surgical guidance. In this paper, we compare the applicability of different seeding and boundary detection methods to NLO microscopic images in order to develop an optimal seeding-based approach capable of accurate segmentation of both TPEF and CARS images. Among different methods, the Laplacian of Gaussian filter showed the best accuracy for the seeding of the image, while a modified seeded watershed segmentation was the most accurate in the task of boundary detection. The resulting combination of these methods followed by the verification of the detected nuclei performs high average sensitivity and specificity when applied to various types of NLO microscopy images.

  20. 7 CFR 201.15 - Weed seeds.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Weed seeds. 201.15 Section 201.15 Agriculture... REGULATIONS Labeling Agricultural Seeds § 201.15 Weed seeds. The percentage of weed seeds shall include seeds of plants considered weeds in the State into which the seed is offered for transportation or...

  1. Vigor-S, a new system for evaluating the physiological potential of maize seeds

    Directory of Open Access Journals (Sweden)

    Danielle Otte Carrara Castan

    Full Text Available ABSTRACT: The refinement of vigor tests and the possibility of utilizing computer resources for the effective evaluation of the seed physiological potential have attracted considerable interest from research and seed technologists. The aim of this study was to evaluate the physiological potential of maize seeds using the newly-created Automated Analysis of Seed Vigor System (Vigor-S compared with other recommended seed vigor tests; two maize hybrids were used, each represented by seven seed lots. Germination and vigor (cold test, saturated salt accelerated aging, and field seedling emergence evaluations were conducted. For the evaluation of seed vigor with the use of seedling image analysis resources, two systems were compared: the Seed Vigor Imaging System (SVIS®, developed by Ohio State University, USA and the Vigor-S, resulting from collaboration between USP/ESALQ and EMBRAPA (Embrapa Instrumentation. Using these two systems, three day old seedlings were scanned and the images were analyzed. Similar results for the vigor index, uniformity of development, and seedling length were obtained. The computerized image analysis of seedlings using Vigor-S has advantages with respect to accuracy, speed, and the possibility of automatic application to a worksheet. It is a consistent alternative for the evaluation of maize seed vigor, and produces information compatible with that obtained by the accelerated aging test and SVIS®.

  2. Potencial fisiológico de sementes de soja e sua relação com a emergência das plântulas em campo = Soybean physiological seed potential and the relationship with seedlings emergence in field

    Directory of Open Access Journals (Sweden)

    Sandra Regina Pelegrinello Schuab

    2006-10-01

    Full Text Available Objetivou-se, nesse estudo, comparar os diferentes testes empregados na avaliação do vigor das sementes de soja e verificar a sua relação com a emergência das plântulas em campo. Assim, o vigor das sementes de soja de dez cultivares foi avaliado por meio dos seguintes testes: emergência das plântulas em campo; germinação; emergência dasplântulas em leito de areia; velocidade de emergência em areia; envelhecimento acelerado; frio modificado; tetrazólio (1-3 e 1-5 e condutividade elétrica. Foi possível observar que os testes de envelhecimento acelerado, germinação, tetrazólio e condutividade elétrica foram os que melhor se correlacionaram com a emergência em campo. Além disso, a interpretação dos resultados obtidos entre os testes de laboratório e de emergência em campo não deve considerar apenas a análise de correlação.The objective of this work was to compare different tests used to evaluate soybean seed vigour and to verify their relationship with seedlings emergence in field. Thus, the soybean seed vigour of ten cultivars was evaluated by the following tests: seedling emergence in field, standard germination, seedlings emergence in sand seedbed, speed of emergence in sand seedbed, accelerated aging, modified cold test, tetrazolium (1-3 and 1-5 and electrical conductivity. The accelerating aging, standard germination, tetrazolium and electrical conductivity tests showed higher correlations with field emergence. The results obtained from laboratory tests and seedlings emergence in field should not consider only the correlation analysis.

  3. Mechanical harvesting of pumpkin seeds

    OpenAIRE

    Sito, Stjepan; Ivančan, Stjepan; Barković, Edi; Mucalo, Ana

    2009-01-01

    One of the key problems in production technology of pumpkin seed for oil production is mechanized harvesting and losses of seed during mechanical harvesting. The losses of pumpkin seed during mechanical harvesting at peripheral velocity of 1.57 m/s (optimally adjusted machine) were 4.4% for Gleisdorf species, 5.2% for Slovenska species and 7.8% for pumpkin with husk. The higher average losses of pumpkin seed with husk were caused by tight connection of seed and pumpkin fruit.

  4. Imaging

    International Nuclear Information System (INIS)

    Kellum, C.D.; Fisher, L.M.; Tegtmeyer, C.J.

    1987-01-01

    This paper examines the advantages of the use of excretory urography for diagnosis. According to the authors, excretory urography remains the basic radiologic examination of the urinary tract and is the foundation for the evaluation of suspected urologic disease. Despite development of the newer diagnostic modalities such as isotope scanning, ultrasonography, CT, and magnetic resonsance imaging (MRI), excretory urography has maintained a prominent role in ruorradiology. Some indications have been altered and will continue to change with the newer imaging modalities, but the initial evaluation of suspected urinary tract structural abnormalities; hematuria, pyuria, and calculus disease is best performed with excretory urography. The examination is relatively inexpensive and simple to perform, with few contraindictions. Excretory urography, when properly performed, can provide valuable information about the renal parenchyma, pelvicalyceal system, ureters, and urinary bladder

  5. Irradiation effect on the seed vigor, SOD activity and MDA content in germinating seeds of yellow-seeded and black-seeded rape seed (Brassica napus L.)

    International Nuclear Information System (INIS)

    Han Jixiang; Hu Danhong; Liu Houli

    1993-01-01

    Seeds of a set of near-isogenic lines (Brassica napus L.) with different seed coat color from yellow to black were irradiated by 60 Co γ-rays of 150 krad. Seed vigor, superoxide dismutase (SOD) and malondialdehyde (MDA) in germinating seeds were analysed. In these characters, no significant difference between yellow-seeded lines (YLs) and black-seeded lines (BLs) showed before irradiation. But after irradiation, SOD activity in YLs was lower than that in BLs. While MDA content in YLs was obviously higher that that in DLs. As a result of irradiation, seed vigor of YLs was lower than that in BLs. these results indicated that the irradiation resistance of rape seed was related to the level of SOD as well as protective structure or substances in seed coat and that the radiosensitivity of YLs was higher than that of DLs

  6. Breeding for Grass Seed Yield

    DEFF Research Database (Denmark)

    Boelt, Birte; Studer, Bruno

    2010-01-01

    Seed yield is a trait of major interest for many fodder and amenity grass species and has received increasing attention since seed multiplication is economically relevant for novel grass cultivars to compete in the commercial market. Although seed yield is a complex trait and affected...... by agricultural practices as well as environmental factors, traits related to seed production reveal considerable genetic variation, prerequisite for improvement by direct or indirect selection. This chapter first reports on the biological and physiological basics of the grass reproduction system, then highlights...... important aspects and components affecting the seed yield potential and the agronomic and environmental aspects affecting the utilization and realization of the seed yield potential. Finally, it discusses the potential of plant breeding to sustainably improve total seed yield in fodder and amenity grasses....

  7. Analysis of Maize Seed Germs by Photoacoustic Microscopy and Photopyroelectric Technique

    Science.gov (United States)

    Pacheco, A. Domínguez; Aguilar, C. Hernández; Cruz-Orea, A.

    2013-05-01

    A knowledge about thermal parameters of structural components of maize seed is of great relevance in the seed technology practice. The objective of the present study was to determine the thermal effusivity of germs of maize ( Zea mays L.) of different genotypes by means of the photopyroelectric technique (PPE) in the inverse configuration and obtaining the thermal imaging of these samples by photoacoustic microscopy (PAM). Germs from crystalline maize (white pigment), semi-crystalline maize (yellow pigment), and floury maize (blue pigment) were used in this investigation. The results show differences between germs of maize seeds mainly in the values of their thermal effusivities. The thermal images showed minimum inhomogeneity of these seed germs. Characterizations of thermal parameters in seeds are important in agriculture and food production and could be particularly useful to define their quality and determine their utility. PPE and PAM can be considered as potential diagnostic tools for the characterization of agriculture seeds.

  8. Physiological quality and seed respiration of primed Jatropha curcas seeds

    Directory of Open Access Journals (Sweden)

    Micheli Angelica Horbach

    2017-11-01

    Full Text Available ABSTRACT Seed deterioration is a natural and irreversible process. Nevertheless, seed priming with water and antioxidants can minimize oxidative damage in oilseeds, resulting in attenuation of seed deterioration. The objective of this assay was to evaluate seed priming on respiratory activity of Jatropha curcas submitted to accelerated aging. Seeds from two provenances (Janauba and Pedro J. Caballero were submitted to three priming treatments (control, immersion in deionized water, and with 750 µmol L-1 of ascorbic acid and treated for accelerated aging at 41 °C for 72 h. The results showed that the priming of J. curcas seeds promoted tolerance to accelerated aging. Primed seeds, with ascorbic acid from Janauba and deionized water from Pedro J. Caballero, resulted in a higher percentage of normal seedlings, and increased germination speed index and seed respiration. The decline of physiological quality of J. curcas seeds after accelerated aging is directly associated with a reduction in respiratory activity that is related to seed moisture content.

  9. Empty seeds are not always bad: simultaneous effect of seed emptiness and masting on animal seed predation.

    Directory of Open Access Journals (Sweden)

    Ramón Perea

    Full Text Available Seed masting and production of empty seeds have often been considered independently as different strategies to reduce seed predation by animals. Here, we integrate both phenomena within the whole assemblage of seed predators (both pre and post-dispersal and in two contrasting microsites (open vs. sheltered to improve our understanding of the factors controlling seed predation in a wind-dispersed tree (Ulmus laevis. In years with larger crop sizes more avian seed predators were attracted with an increase in the proportion of full seeds predated on the ground. However, for abundant crops, the presence of empty seeds decreased the proportion of full seeds predated. Empty seeds remained for a very long period in the tree, making location of full seeds more difficult for pre-dispersal predators and expanding the overall seed drop period at a very low cost (in dry biomass and allocation of C, N and P. Parthenocarpy (non-fertilized seeds was the main cause of seed emptiness whereas seed abortion was produced in low quantity. These aborted seeds fell prematurely and, thus, could not work as deceptive seeds. A proportion of 50% empty seeds significantly reduced ground seed predation by 26%. However, a high rate of parthenocarpy (beyond 50% empty seeds did not significantly reduce seed predation in comparison to 50% empty seeds. We also found a high variability and unpredictability in the production of empty seeds, both at tree and population level, making predator deception more effective. Open areas were especially important to facilitate seed survival since rodents (the main post-dispersal predators consumed seeds mostly under shrub cover. In elm trees parthenocarpy is a common event that might work as an adaptive strategy to reduce seed predation. Masting per se did not apparently reduce the overall proportion of seeds predated in this wind-dispersed tree, but kept great numbers of seeds unconsumed.

  10. Seeds of the Future

    CERN Multimedia

    CERN. Geneva

    2015-01-01

    Five of the global issues most frequently debated today are the decline of biodiversity in general and of agrobiodiversity in particular, climate change, hunger and malnutrition, poverty and water. These issues are connected with each other, and should be dealt with as such. Most of our food comes from seeds (even when we eat meat, we indirectly eat plants, which come from seeds) and food affects our health. The evolution of plant breeding, the science which is responsible for the type and the diversity of seed that farmers plant, and hence for the diversity of food that we eat, helps us understand how agrobiodiversity has decreased. An agro-ecological model of agriculture could be solution to the most important problems affecting the planet, but is often criticized for not being able to produce enough food for a growing population casting doubts on whether food security and food safety can be compatible objectives. Participatory and evolutionary plant breeding, while benefiting from advances in molecular g...

  11. Physicochemical Evaluation of Seeds and Oil of Nontraditional Oil Seeds

    Directory of Open Access Journals (Sweden)

    Adam Ismail Ahmed

    2015-08-01

    Full Text Available The present work was conducted in the Laboratory of Biochemistry and Food science department, Faculty of Natural Resources and Environmental Studies, University of Kordofan, in order to evaluate some nontraditional oil seeds these are i.e. Marula (Sclerocarya birrea, Roselle (Hibiscus sabdariffa L. seeds and Christ’s thorn (Zizyphus spina-christi seeds. The seeds of the roselle and Christ’s thorn fruits were procured from Elobeid local market, North Kordofan State, while marula fruits were obtained from Elnuhod, West Kordofan State. The proximate composition of the seeds, cake and christ’s thorn pulp was done. Some chemical and physical properties were performed for the extracted oil. The results revealed that proximate composition of the seeds and cake differ statistically among the studied materials. Significant differences were observed among the oil extracted from these species; moreover, these oils differ significantly in color and viscosity only.

  12. Sunflower seed allergy

    Science.gov (United States)

    Ukleja-Sokołowska, Natalia; Gawrońska-Ukleja, Ewa; Żbikowska-Gotz, Magdalena; Bartuzi, Zbigniew; Sokołowski, Łukasz

    2016-01-01

    Sunflower seeds are a rare source of allergy, but several cases of occupational allergies to sunflowers have been described. Sunflower allergens on the whole, however, still await precise and systematic description. We present an interesting case of a 40-year-old male patient, admitted to hospital due to shortness of breath and urticaria, both of which appeared shortly after the patient ingested sunflower seeds. Our laryngological examination revealed swelling of the pharynx with retention of saliva and swelling of the mouth and tongue. During diagnostics, 2 months later, we found that skin prick tests were positive to mugwort pollen (12/9 mm), oranges (6/6 mm), egg protein (3/3 mm), and hazelnuts (3/3 mm). A native prick by prick test with sunflower seeds was strongly positive (8/5 mm). Elevated concentrations of specific IgE against weed mix (inc. lenscale, mugwort, ragweed) allergens (1.04 IU/mL), Artemisia vulgaris (1.36 IU/mL), and Artemisia absinthium (0.49 IU/mL) were found. An ImmunoCap ISAC test found an average level of specific IgE against mugwort pollen allergen component Art v 1 - 5,7 ISU-E, indicating an allergy to mugwort pollen and low to medium levels of specific IgE against lipid transfer proteins (LTP) found in walnuts, peanuts, mugwort pollen, and hazelnuts. Through the ISAC inhibition test we proved that sunflower seed allergen extracts contain proteins cross-reactive with patients’ IgE specific to Art v 1, Art v 3, and Jug r 3. Based on our results and the clinical pattern of the disease we confirmed that the patient is allergic to mugwort pollen and that he had an anaphylactic reaction as a result of ingesting sunflower seeds. We suspected that hypersensitivity to sunflower LTP and defensin-like proteins, both cross-reactive with mugwort pollen allergens, were the main cause of the patient’s anaphylactic reaction. PMID:27222528

  13. Tratamento fungicida de sementes de soja inoculadas com Colletotrichum truncatum Fungicide treatment of soybean seeds inoculated with Colletotrichum truncatum

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Pereira

    2009-12-01

    whitout polymer. Seed germination, cold test, seedling emergence and seed health test (blotter test were evaluated. Plants grown in pots were evaluated using length of aerial parts, dry matter of roots and aerial parts. The best control of C. truncatum was obtained when the seeds were treated with fludioxonil+mefenoxan and thiabendazole+thiram. These treatments also contributed to improve the physiological performance of the seeds. The soybean seeds vigour and the plants growth were reduced when the seeds are treated whit methyl thiophanate. The film-coating do not affect the physiological potential and the soybean seed health.

  14. Epitaxially grown polycrystalline silicon thin-film solar cells on solid-phase crystallised seed layers

    Energy Technology Data Exchange (ETDEWEB)

    Li, Wei, E-mail: weili.unsw@gmail.com; Varlamov, Sergey; Xue, Chaowei

    2014-09-30

    Highlights: • Crystallisation kinetic is used to analyse seed layer surface cleanliness. • Simplified RCA cleaning for the seed layer can shorten the epitaxy annealing duration. • RTA for the seed layer can improve the quality for both seed layer and epi-layer. • Epitaxial poly-Si solar cell performance is improved by RTA treated seed layer. - Abstract: This paper presents the fabrication of poly-Si thin film solar cells on glass substrates using seed layer approach. The solid-phase crystallised P-doped seed layer is not only used as the crystalline template for the epitaxial growth but also as the emitter for the solar cell structure. This paper investigates two important factors, surface cleaning and intragrain defects elimination for the seed layer, which can greatly influence the epitaxial grown solar cell performance. Shorter incubation and crystallisation time is observed using a simplified RCA cleaning than the other two wet chemical cleaning methods, indicating a cleaner seed layer surface is achieved. Cross sectional transmission microscope images confirm a crystallographic transferal of information from the simplified RCA cleaned seed layer into the epi-layer. RTA for the SPC seed layer can effectively eliminate the intragrain defects in the seed layer and improve structural quality of both of the seed layer and the epi-layer. Consequently, epitaxial grown poly-Si solar cell on the RTA treated seed layer shows better solar cell efficiency, V{sub oc} and J{sub sc} than the one on the seed layer without RTA treatment.

  15. Seed morphometric characteristics of European species of Elatine (Elatinaceae

    Directory of Open Access Journals (Sweden)

    Agnieszka Popiela

    2017-05-01

    Full Text Available Elatine L. contains ca. 25 small, herbaceous, annual species distributed in ephemeral waters in both hemispheres. All species are amphibious and characterized by a high degree of morphological variability. The importance of seed morphology in Elatine taxonomy has been emphasized by many authors. The degree of seed curvature and seed coat reticulation have been traditionally considered very important in recognizing individual species of this genus. Seed morphometric characteristics of 10 Elatine species, including all European native taxa, are provided on the basis of material from two or three populations of each species. A total of 24–50 seeds were studied from each population, altogether 1,260 images were used for the morphometric study. In total, six parameters were measured from SEM pictures: object surface area, profile specific perimeter (object circuit, rectangle of the object (a length, rectangle of the object (b width, angle of the seed curvature, and number of pits in the seed coat counted in the middle row. Our study shows that the range of morphological variation of seeds in European species of Elatine is great, both between the species and the populations. Discrimination analysis showed that all six traits significantly differentiate the populations studied (λ = 0.001, p < 0.001, and the greatest contributions were “number of pits”, “rectangle_a”, and “the angle curvature”. Multidimensional scaling based on a correlation matrix of Mahalanobis distance of the six features studied revealed the greatest similarity between the three populations of E. alsinastrum, E. macropoda, and E. hexandra. Regarding interspecific differences, a Kruskal–Wallis tests showed that, in many cases, lack of statistically significant differences between species relative to the studied seed traits. If distinction of species is only based on seeds, especially if only a few seeds are evaluated, the following species pairs can be easily

  16. Correlação entre testes para avaliação da qualidade de sementes de girassol e emergência das plântulas em campo Correlation between sunflower seeds quality evaluation tests and seedling emergence in field

    Directory of Open Access Journals (Sweden)

    Madelon Rodrigues Sá Braz

    2009-10-01

    Full Text Available A capacidade do teste de germinação para estimar o potencial de emergência de plântulas no campo diminui à medida que as condições do ambiente são desfavoráveis. No entanto, diferentes testes de vigor têm sido utilizados para identificar os lotes de sementes que apresentam melhor desempenho nessas situações. O objetivo do trabalho foi correlacionar os resultados dos testes conduzidos no laboratório com a emergência das plântulas de girassol em campo. Para isso, cinco lotes de sementes de girassol da cultivar 'Embrapa 122 V2000' foram submetidos aos testes de germinação e vigor (primeira contagem, classificação de plântulas, teste de frio, condutividade elétrica, emergência de plântulas em areia e em campo, índice de velocidade de emergência, envelhecimento acelerado e deterioração controlada. Em condições adversas, a emergência das plântulas de girassol em campo apresentou maior correlação com o vigor, avaliado pelos testes de envelhecimento acelerado, de deterioração controlada e de condutividade elétrica.The germination capacity test to estimate the potential of seedlings emergence in the field decreases when the environmental conditions are unfavorable. However, different vigour tests have been used to identify the seed lots that have better perform in these situations. The objective of this research was to correlate the results of tests conducted in the laboratory with the emergence of sunflower seedlings in the field. For this, five lots of seeds sunflower cultivar 'Embrapa 122 V2000' were submitted to the germination and vigour tests (first count, classification of seedlings, cold test, electrical conductivity, seedling emergence in sand and in the field, emergence speed index, accelerated aging and controlled deterioration. Under adverse conditions, the seed vigour evaluated by accelerated aging, controlled deterioration and electrical conductivity presented higher correlation with sunflower plant

  17. In vivo monitoring of seeds and plant-tissue water absorption using optical coherence tomography and optical coherence microscopy

    Science.gov (United States)

    Sapozhnikova, Veronika V.; Kutis, Irina S.; Kutis, Sergey D.; Kuranov, Roman V.; Gelikonov, Grigory V.; Shabanov, Dmitry V.; Kamensky, Vladislav A.

    2004-07-01

    First experimental results on OCT imaging of internal structure of plant tissues and in situ OCT monitoring of plant tissue regeneration at different water supply are reported. Experiments for evaluating OCT capabilities were performed on Tradescantia. The investigation of seeds swelling was performed on wheat seeds (Triticum L.), barley seeds (Hordeum L.), long-fibred flax seeds (Linum usitatissimum L.) and cucumber seeds (Cucumis sativus L.). These OCT images correlate with standard microscopy data from the same tissue regions. Seeds were exposed to a low-intensity physical factor-the pulsed gradient magnetic field (GMF) with pulse duration 0.1 s and maximum amplitude 5 mT (4 successive pulses during 0.4 s). OCT and OCM enable effective monitoring of fast reactions in plants and seeds at different water supply.

  18. Reducing the random seed effect on segmentation by applying an edge-preserving filter

    NARCIS (Netherlands)

    Addink, E.A.

    2012-01-01

    In region-growing segmentation algorithms random seed locations are used (reference). To ensure that repeating the segmentation will produce the same result, the seed locations are following a fixed random pattern. Empirical studies show that when the image that is subjected to the segmentation is

  19. Pathogenic mycoflora on carrot seeds

    Directory of Open Access Journals (Sweden)

    Bogdan Nowicki

    2013-12-01

    Full Text Available Altogether 300 seed samples were collected during 9 years in 8 regions of Poland and the fungi Were isolated and their pathogenicity to carrot seedlings was examined. Alternaria rudicina provcd to be the most important pathogen although. A. alternata was more common. The other important pathogens were Fusarium spp., Phoma spp. and Botrytis cinerea. The infection of carrot seeds by A. radicina should be used as an important criterium in seed quality evaluation.

  20. Maturation of sugar maple seed

    Science.gov (United States)

    Clayton M., Jr. Carl; Albert G., Jr. Snow; Albert G. Snow

    1971-01-01

    The seeds of a sugar maple tree (Acer saccharum Marsh.) do not mature at the same time every year. And different trees mature their seeds at different times. So time of year is not a reliable measure of when seeds are ripe. Better criteria are needed. In recent studies we have found that moisture content and color are the best criteria for judging when sugar maple...

  1. Citricoccus zhacaiensis B-4 (MTCC 12119) a novel osmotolerant plant growth promoting actinobacterium enhances onion (Allium cepa L.) seed germination under osmotic stress conditions.

    Science.gov (United States)

    Selvakumar, Govindan; Bhatt, Ravindra M; Upreti, Kaushal K; Bindu, Gurupadam Hema; Shweta, Kademani

    2015-05-01

    The water potential of rhizospheric soil is a key parameter that determines the availability of water, oxygen, and nutrients to plants and microbes. Recent global warming trends and erratic precipitation patterns have resulted in the emergence of drought as a major constraint of agricultural productivity. Though several strategies are being evaluated to address this issue, a novel approach is the utilization of microbes for alleviation of drought stress effects in crops. Citricoccus zhacaiensis B-4 is an osmotolerant actinobacterium isolated from banana rhizosphere on mannitol supplemented medium (-2.92 MPa osmotic potential). This isolate expressed plant growth promotion traits viz, IAA, GA3 production, phosphate, zinc solubilization, ACC deaminase activity and ammonia production under PEG induced osmotic stress and non-stress conditions. Under in vitro osmotic conditions, biopriming with the actinobacterium improved the percent germination, seedling vigour and germination rate of onion seeds (cv. Arka Kalyan) at osmotic potentials up to -0.8 MPa. Considering its novelty, osmotolerance and plant growth promoting traits, biopriming with C. zhacaiensis is suggested as a viable option for the promotion of onion seed germination under drought stressed environments.

  2. Within canopy distribution of cotton seed N

    Science.gov (United States)

    Whole cotton seeds can be an important component of dairy rations. Nitrogen content of the seed is an important determinant of the feed value of the seed. Efforts to increase the seed value as feed will be enhanced with knowledge of the range and distribution of seed N within the cotton crop. This s...

  3. Grau de umidade e temperatura na conservação de sementes de café Moisture content and temperature on the preservation of coffee seeds

    Directory of Open Access Journals (Sweden)

    DANIEL FELIPE DE OLIVEIRA GENTIL

    2001-01-01

    Full Text Available O presente trabalho foi realizado com o objetivo de verificar as influências do grau de umidade e da temperatura de armazenamento na manutenção da qualidade de sementes de Coffea arabica L. Sementes com 51%, 41%, 34%, 23%, 16% e 10% de água, acondicionadas em sacos de polietileno e mantidas sob temperaturas de 30 °C, 20 °C e 10 °C, durante 48 semanas de armazenamento, foram submetidas a avaliações periódicas do grau de umidade, da germinação, do vigor e da sanidade. Foi constatado que as reduções do grau de umidade até 10% e da temperatura até 10 °C são favoráveis à manutenção da qualidade fisiológica das sementes, e que umidade próxima a 23% favorece o estabelecimento de Penicillium sp. e de Aspergillus sp. nas sementes.The aim of this work was to verify the influences of the moisture content and storage temperature in the preservation of Coffea arabica L. seeds. Seeds with 51%, 41%, 34%, 23%, 16% and 10% moisture content were packed in polyethylene bags and stored at temperatures of 30 °C, 20 °C and 10 °C for forty-eight weeks, and submitted to periodic evaluations of moisture content, germination, vigour and sanity. Decreases in moisture content until 10% and temperature until 10 °C were favorable to the maintenance of seed physiological quality. Seed moisture content around 23% favor the establishment of Penicillium sp. and Aspergillus sp.

  4. 19 CFR 10.57 - Certified seed potatoes, and seed corn or maize.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Certified seed potatoes, and seed corn or maize... Provisions Potatoes, Corn, Or Maize § 10.57 Certified seed potatoes, and seed corn or maize. Claim for classification as seed potatoes under subheading 0701.10.00, as seed corn (maize) under subheading 1005.10...

  5. Seed dormancy and seed longevity: from genetic variation to gene identification

    NARCIS (Netherlands)

    Nguyen, T.P.

    2014-01-01

    Seed dormancy and seed longevity are the most important survival traits in the soil seed bank. Both traits are induced during seed maturation and evolved to assure seed survival during environmental conditions that cannot support the regular course of life. Seed dormancy is related to the timing of

  6. Why high seed densities within buried mesh bags may overestimate depletion rates of soil seed banks

    NARCIS (Netherlands)

    Mourik, van T.A.; Stomph, T.J.; Murdoch, A.J.

    2005-01-01

    1. Estimates of seed bank depletion rates are essential for modelling and management of plant populations. The seed bag burial method is often used to measure seed mortality in the soil. However, the density of seeds within seed bags is higher than densities in natural seed banks, which may elevate

  7. Heirloom biodynamic seeds network rescue, conservation and multiplication of local seeds in Brazil

    OpenAIRE

    Jovchelevich, Pedro

    2014-01-01

    Structuring a network organic and biodynamic seed involving farmers in the central- southern Brazil. Training, participatory breeding, edition of publications, fairs of exchange seeds, a processing unit and assessment of seed quality, commercial seed multiplication with emphasis on vegetables. This network has garanteed the autonomy of farmers in seed production and enriched agrobiodiversity through exchanges of seed.

  8. Seed drill depth control system for precision seeding

    DEFF Research Database (Denmark)

    Kirkegaard Nielsen, Søren; Munkholm, Lars Juhl; Lamandé, Mathieu

    2018-01-01

    acting on the drill coulters, which generates unwanted vibrations and, consequently, a non-uniform seed placement. Therefore, a proof-of-concept dynamic coulter depth control system for a low-cost seed drill was developed and studied in a field experiment. The performance of the active control system...... depth control system this variability was reduced to±2 mm. The system with the active control system operated more accurately at an operational speed of 12 km h−1 than at 4 km h−1 without the activated control system.......An adequate and uniform seeding depth is crucial for the homogeneous development of a crop, as it affects time of emergence and germination rate. The considerable depth variations observed during seeding operations - even for modern seed drills - are mainly caused by variability in soil resistance...

  9. High-Throughput Scoring of Seed Germination.

    Science.gov (United States)

    Ligterink, Wilco; Hilhorst, Henk W M

    2017-01-01

    High-throughput analysis of seed germination for phenotyping large genetic populations or mutant collections is very labor intensive and would highly benefit from an automated setup. Although very often used, the total germination percentage after a nominated period of time is not very informative as it lacks information about start, rate, and uniformity of germination, which are highly indicative of such traits as dormancy, stress tolerance, and seed longevity. The calculation of cumulative germination curves requires information about germination percentage at various time points. We developed the GERMINATOR package: a simple, highly cost-efficient, and flexible procedure for high-throughput automatic scoring and evaluation of germination that can be implemented without the use of complex robotics. The GERMINATOR package contains three modules: (I) design of experimental setup with various options to replicate and randomize samples; (II) automatic scoring of germination based on the color contrast between the protruding radicle and seed coat on a single image; and (III) curve fitting of cumulative germination data and the extraction, recap, and visualization of the various germination parameters. GERMINATOR is a freely available package that allows the monitoring and analysis of several thousands of germination tests, several times a day by a single person.

  10. Practical considerations for maximizing heat production in a novel thermobrachytherapy seed prototype

    Energy Technology Data Exchange (ETDEWEB)

    Gautam, Bhoj; Warrell, Gregory; Shvydka, Diana; Ishmael Parsai, E., E-mail: e.parsai@utoledo.edu [University of Toledo Medical Center, 3000 Arlington Avenue, MS1151, Toledo, Ohio 43614 (United States); Subramanian, Manny [BEST Medical International, Inc., 7643 Fullerton Road, Springfield, Virginia 22153 (United States)

    2014-02-15

    Purpose: A combination of hyperthermia and radiation in the treatment of cancer has been proven to provide better tumor control than radiation administered as a monomodality, without an increase in complications or serious toxicities. Moreover, concurrent administration of hyperthermia and radiation displays synergistic enhancement, resulting in greater tumor cell killing than hyperthermia and radiation delivered separately. The authors have designed a new thermobrachytherapy (TB) seed, which serves as a source of both radiation and heat for concurrent brachytherapy and hyperthermia treatments when implanted in solid tumors. This innovative seed, similar in size and geometry to conventional seeds, will have self-regulating thermal properties. Methods: The new seed's geometry is based on the standard BEST Model 2301{sup 125}I seed, resulting in very similar dosimetric properties. The TB seed generates heat when placed in an oscillating magnetic field via induction heating of a ferromagnetic Ni–Cu alloy core that replaces the tungsten radiographic marker of the standard Model 2301. The alloy composition is selected to undergo a Curie transition near 50 °C, drastically decreasing power production at higher temperatures and providing for temperature self-regulation. Here, the authors present experimental studies of the magnetic properties of Ni–Cu alloy material, the visibility of TB seeds in radiographic imaging, and the ability of seed prototypes to uniformly heat tissue to a desirable temperature. Moreover, analyses are presented of magnetic shielding and thermal expansion of the TB seed, as well as matching of radiation dose to temperature distributions for a short interseed distance in a given treatment volume. Results: Annealing the Ni–Cu alloy has a significant effect on its magnetization properties, increasing the sharpness of the Curie transition. The TB seed preserves the radiographic properties of the BEST 2301 seed in both plain x rays and CT

  11. Practical considerations for maximizing heat production in a novel thermobrachytherapy seed prototype

    International Nuclear Information System (INIS)

    Gautam, Bhoj; Warrell, Gregory; Shvydka, Diana; Ishmael Parsai, E.; Subramanian, Manny

    2014-01-01

    Purpose: A combination of hyperthermia and radiation in the treatment of cancer has been proven to provide better tumor control than radiation administered as a monomodality, without an increase in complications or serious toxicities. Moreover, concurrent administration of hyperthermia and radiation displays synergistic enhancement, resulting in greater tumor cell killing than hyperthermia and radiation delivered separately. The authors have designed a new thermobrachytherapy (TB) seed, which serves as a source of both radiation and heat for concurrent brachytherapy and hyperthermia treatments when implanted in solid tumors. This innovative seed, similar in size and geometry to conventional seeds, will have self-regulating thermal properties. Methods: The new seed's geometry is based on the standard BEST Model 2301 125 I seed, resulting in very similar dosimetric properties. The TB seed generates heat when placed in an oscillating magnetic field via induction heating of a ferromagnetic Ni–Cu alloy core that replaces the tungsten radiographic marker of the standard Model 2301. The alloy composition is selected to undergo a Curie transition near 50 °C, drastically decreasing power production at higher temperatures and providing for temperature self-regulation. Here, the authors present experimental studies of the magnetic properties of Ni–Cu alloy material, the visibility of TB seeds in radiographic imaging, and the ability of seed prototypes to uniformly heat tissue to a desirable temperature. Moreover, analyses are presented of magnetic shielding and thermal expansion of the TB seed, as well as matching of radiation dose to temperature distributions for a short interseed distance in a given treatment volume. Results: Annealing the Ni–Cu alloy has a significant effect on its magnetization properties, increasing the sharpness of the Curie transition. The TB seed preserves the radiographic properties of the BEST 2301 seed in both plain x rays and CT images

  12. Clinical application and validation of an iterative forward projection matching algorithm for permanent brachytherapy seed localization from conebeam-CT x-ray projections

    Energy Technology Data Exchange (ETDEWEB)

    Pokhrel, Damodar; Murphy, Martin J.; Todor, Dorin A.; Weiss, Elisabeth; Williamson, Jeffrey F. [Department of Radiation Oncology, School of Medicine, Virginia Commonwealth University, Richmond, Virginia 23298 (United States)

    2010-09-15

    Purpose: To experimentally validate a new algorithm for reconstructing the 3D positions of implanted brachytherapy seeds from postoperatively acquired 2D conebeam-CT (CBCT) projection images. Methods: The iterative forward projection matching (IFPM) algorithm finds the 3D seed geometry that minimizes the sum of the squared intensity differences between computed projections of an initial estimate of the seed configuration and radiographic projections of the implant. In-house machined phantoms, containing arrays of 12 and 72 seeds, respectively, are used to validate this method. Also, four {sup 103}Pd postimplant patients are scanned using an ACUITY digital simulator. Three to ten x-ray images are selected from the CBCT projection set and processed to create binary seed-only images. To quantify IFPM accuracy, the reconstructed seed positions are forward projected and overlaid on the measured seed images to find the nearest-neighbor distance between measured and computed seed positions for each image pair. Also, the estimated 3D seed coordinates are compared to known seed positions in the phantom and clinically obtained VariSeed planning coordinates for the patient data. Results: For the phantom study, seed localization error is (0.58{+-}0.33) mm. For all four patient cases, the mean registration error is better than 1 mm while compared against the measured seed projections. IFPM converges in 20-28 iterations, with a computation time of about 1.9-2.8 min/iteration on a 1 GHz processor. Conclusions: The IFPM algorithm avoids the need to match corresponding seeds in each projection as required by standard back-projection methods. The authors' results demonstrate {approx}1 mm accuracy in reconstructing the 3D positions of brachytherapy seeds from the measured 2D projections. This algorithm also successfully localizes overlapping clustered and highly migrated seeds in the implant.

  13. Clinical application and validation of an iterative forward projection matching algorithm for permanent brachytherapy seed localization from conebeam-CT x-ray projections.

    Science.gov (United States)

    Pokhrel, Damodar; Murphy, Martin J; Todor, Dorin A; Weiss, Elisabeth; Williamson, Jeffrey F

    2010-09-01

    To experimentally validate a new algorithm for reconstructing the 3D positions of implanted brachytherapy seeds from postoperatively acquired 2D conebeam-CT (CBCT) projection images. The iterative forward projection matching (IFPM) algorithm finds the 3D seed geometry that minimizes the sum of the squared intensity differences between computed projections of an initial estimate of the seed configuration and radiographic projections of the implant. In-house machined phantoms, containing arrays of 12 and 72 seeds, respectively, are used to validate this method. Also, four 103Pd postimplant patients are scanned using an ACUITY digital simulator. Three to ten x-ray images are selected from the CBCT projection set and processed to create binary seed-only images. To quantify IFPM accuracy, the reconstructed seed positions are forward projected and overlaid on the measured seed images to find the nearest-neighbor distance between measured and computed seed positions for each image pair. Also, the estimated 3D seed coordinates are compared to known seed positions in the phantom and clinically obtained VariSeed planning coordinates for the patient data. For the phantom study, seed localization error is (0.58 +/- 0.33) mm. For all four patient cases, the mean registration error is better than 1 mm while compared against the measured seed projections. IFPM converges in 20-28 iterations, with a computation time of about 1.9-2.8 min/ iteration on a 1 GHz processor. The IFPM algorithm avoids the need to match corresponding seeds in each projection as required by standard back-projection methods. The authors' results demonstrate approximately 1 mm accuracy in reconstructing the 3D positions of brachytherapy seeds from the measured 2D projections. This algorithm also successfully localizes overlapping clustered and highly migrated seeds in the implant.

  14. 7 CFR 201.50 - Weed seed.

    Science.gov (United States)

    2010-01-01

    ... REGULATIONS Purity Analysis in the Administration of the Act § 201.50 Weed seed. Seeds (including bulblets or... sieve are considered weed seeds. For wild onion and wild garlic (Allium spp.) bulblets classed as inert...

  15. Seeding and planting upland oaks

    Science.gov (United States)

    1989-01-01

    Oaks can be planted or seeded in uplands to: (1) afforest old fields, strip-mined areas, or other areas devoid of trees, and (2) supplement natural reproduction within existing forests. Planting is usually more successful than direct seeding. But even under good conditions survival and growth of planted oak has been considerably poorer than with conifers and other...

  16. STORAGE OF Handroanthus umbellatus SEEDS

    Directory of Open Access Journals (Sweden)

    Cibele Chalita Martins

    2014-09-01

    Full Text Available http://dx.doi.org/10.5902/1980509815725Seed storage under controlled environmental conditions represents one of the most important lines of research to be applied on short-lived forest species as Handroanthus. The present research aimed to identify the most suitable seed storage conditions and longevity behavior of Handroanthus umbellatus seeds subject to the following storage treatments: packaging permeable paper bags under a no-controlled laboratory temperature and humidity (control and multiwall semipermeable bag at temperatures of -18 ºC, 1 ºC and 25 ºC. Seeds were dried to 6.3% of water content. Stored seeds were evaluated every three months until 24 months for water content, germination percentage and vigor utilizing first counting test. Seeds of T. umbellata are orthodox, with low longevity under natural conditions, once they remain viable for less than 5 months. The best conditions of seed preservation of these species were obtained by storage at -18° C in multiwall bags. Under these conditions physiological seed quality remains unchanged for a 24-month period.

  17. Uganda Early Generation Seed Study

    NARCIS (Netherlands)

    Mastenbroek, A.; Ntare, Bonny

    2016-01-01

    One of the major bottlenecks limiting farmers’ access to good quality seed for food crops in Uganda is the shortage of early generation seed (EGS - breeder and foundation) to produce sufficient quantities of certified and/or quality declared) to satisfy the needs of farmers. A national study was

  18. Insecticide seed treatments for sugarbeet

    Science.gov (United States)

    Pest feeding and vectoring of viruses cause serious problems in sugarbeet production worldwide. In order to ameliorate pest and disease problems on sugarbeet, two seed treatments, Poncho Beta (60 g a.i. clothianidin + 8 g a.i. beta-cyfluthrin/100,000 seed) and Cruiser Tef (60 g a.i. thiamethoxam + 8...

  19. Orthodox seeds and resurrection plants

    NARCIS (Netherlands)

    Costa, Maria Cecília Dias; Cooper, Keren; Hilhorst, Henk W.M.; Farrant, Jill M.

    2017-01-01

    Although staple crops do not survive extended periods of drought, their seeds possess desiccation tolerance (DT), as they survive almost complete dehydration (desiccation) during the late maturation phase of development. Resurrection plants are plant species whose seeds and vegetative tissues are

  20. Efficient computation of spaced seeds

    Directory of Open Access Journals (Sweden)

    Ilie Silvana

    2012-02-01

    Full Text Available Abstract Background The most frequently used tools in bioinformatics are those searching for similarities, or local alignments, between biological sequences. Since the exact dynamic programming algorithm is quadratic, linear-time heuristics such as BLAST are used. Spaced seeds are much more sensitive than the consecutive seed of BLAST and using several seeds represents the current state of the art in approximate search for biological sequences. The most important aspect is computing highly sensitive seeds. Since the problem seems hard, heuristic algorithms are used. The leading software in the common Bernoulli model is the SpEED program. Findings SpEED uses a hill climbing method based on the overlap complexity heuristic. We propose a new algorithm for this heuristic that improves its speed by over one order of magnitude. We use the new implementation to compute improved seeds for several software programs. We compute as well multiple seeds of the same weight as MegaBLAST, that greatly improve its sensitivity. Conclusion Multiple spaced seeds are being successfully used in bioinformatics software programs. Enabling researchers to compute very fast high quality seeds will help expanding the range of their applications.

  1. Kauri seeds and larval somersaults

    DEFF Research Database (Denmark)

    Dupont, Steen Thorleif

    2012-01-01

    The trunk morphology of the larvae of the kauri pine (Agathis) seed infesting moth Agathiphaga is described using conventional, polarization, and scanning electron microscopy. The pine seed chamber formed by the larva is also described and commented on. The simple larval chaetotaxy includes more ...

  2. Banking Wyoming big sagebrush seeds

    Science.gov (United States)

    Robert P. Karrfalt; Nancy Shaw

    2013-01-01

    Five commercially produced seed lots of Wyoming big sagebrush (Artemisia tridentata Nutt. var. wyomingensis (Beetle & Young) S.L. Welsh [Asteraceae]) were stored under various conditions for 5 y. Purity, moisture content as measured by equilibrium relative humidity, and storage temperature were all important factors to successful seed storage. Our results indicate...

  3. Vigor of sunflower and soybean aging seed

    OpenAIRE

    Tatić M.; Balešević-Tubić S.; Ðorđević V.; Miklič V.; Vujaković M.; Ðukić V.

    2012-01-01

    Seed aging and deterioration affect seed vigor and viability. The characteristics of the chemical composition of oil crops seed are related to specific processes occurring in the seed during storage. This study was performed to examine the changes in seed vigor of different sunflower and soybean genotypes under controlled and conventional (uncontrolled) conditions of natural aging for six and twelve months. Obtained results show that the degree of seed dama...

  4. Plasma treatment of Seeds: effect on growth, spores and bacterial charge

    Science.gov (United States)

    Ambrico, P. F.; Simek, M.; Morano, M.; Ambrico, M.; Minafra, A.; Prukner, V.; de Miccolis Angelini, R. M.; Trotti, P.

    2016-09-01

    We report on the effect of low temperature plasma treatment on tomato, basil and tobacco commercial seeds. Seeds were treated in filtered ambient air volume, surface and plasma jet DBD at atmospheric pressure Sterile agar substrate, supplemented with a nutrient and vitamin mixture, was used to allow seeds germination in sterilized sealed plastic containers. The seeds were stored in controlled environmental condition (T = 26C, cycle of 14hrs light/10hrs dark condition). Since all the procedure was performed under sterile conditions, only bacteria and fungi carried by seeds could grow. Plasma treatment significantly reduced the presence of bacterial contamination, while some fungi could resist at shortest exposures Seeds germination was then followed by time lapse photography in sterile water on 3MM Whatman paper in a closed container. The effect of plasma treatment was a faster germination time of seeds and emergence of cotyledons, able to start photosynthesis in seedlings.The plasma treated seeds were also sow in a soil/peat moss mixture. Plants were cultivated for about 40 days, showing that plasma induced a faster growth in length and weight with respect to untreated seeds.Furthermore the effect of plasma on seeds surface was studied by SEM imaging. We acknowledge `SELGE' (Puglia) and TACR (TA03010098).

  5. Three-dimensional seed reconstruction from an incomplete data set for prostate brachytherapy

    International Nuclear Information System (INIS)

    Narayanan, Sreeram; Cho, Paul S; MarksII, Robert J

    2004-01-01

    Intra-operative dosimetry in prostate brachytherapy requires 3D coordinates of the implanted, radioactive seeds. Since CT is not readily available during the implant operation, projection x-rays are commonly used for intra-operative seed localization. Three x-ray projections are usually used. The requirement of the current seed reconstruction algorithms is that the seeds must be identified on all three projections. However, in practice this is often difficult to accomplish due to the problem of heavily clustered and overlapping seeds. We have developed an algorithm that permits seed reconstruction from an incomplete data set. Instead of all three projections, the new algorithm requires only one of the three projections to be complete. Furthermore, even if all three projections are incomplete, it can reconstruct 100% of the implanted seeds depending on how the undetected seeds are distributed among the projections. The method utilizes the principles of epipolar imaging geometry and pseudo-matching of the undetected seeds. The algorithm was successfully applied to a large number of clinical cases where seeds imperceptibly overlap in some projections

  6. Wheat seed system in Ethiopia: Farmers' varietal perception, seed sources, and seed management

    NARCIS (Netherlands)

    Bishaw, Z.; Struik, P.C.; Gastel, van A.J.G.

    2010-01-01

    Knowledge and information on farmers' perception and its influence on adoption of modern wheat varieties, awareness and source of new wheat production technology, wheat seed sources, and on-farm seed-management practices remain sporadic in Ethiopia. This study was conducted to understand the

  7. Farmer’s seed sources and seed quality: 2. seed health

    NARCIS (Netherlands)

    Bishaw, Z.; Struik, P.C.; Gastel, van A.J.G.

    2013-01-01

    The study assessed the health quality of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) seed samples collected from formal and informal sector in Ethiopia and Syria. In Ethiopia, several seed-borne fungi were found on wheat samples: Cochliobolus sativum, Fusarium avenaceum, F.

  8. Size, physiological quality, and green seed occurrence influenced by seeding rate in soybeans

    Directory of Open Access Journals (Sweden)

    André Sampaio Ferreira

    2017-05-01

    Full Text Available The seeding rate influences the intraspecific competition, which might affect the development and quality of seeds in soybean. However, the impact of seeding rate on the physical and physiological qualities of soybean seeds needs to be better elucidated. This study aimed to evaluate the effects of soybean plant density on the seed size as well as the effects of the interaction between the plant density and seed size on the seed mass, green seed occurence, and physiological seed quality. The experiments were carried out in the growing seasons of the years 2013/14 and 2014/15 in a Latossolo Vermelho distroférrico, under a randomized complete block design, using the NK 7059 RR cultivar with six replications. Four plant densities (150, 300, 440, and 560 thousand viable seeds ha–1 were evaluated. After the classification of seeds into four sizes, using a set of sieves, a 4 ×4 factorial scheme was used for the statistical analysis of the four plant densities and four seed sizes. The seed samples were evaluated for the seed mass, green seed percentage, germination, and vigor. Under thermal and water stress during seed development, an increase in the seeding rate led to a reduction in the green seed occurrence and an increase in the seed size and mass. However, in the absence of thermal and water stress, the seed size and mass were not altered by the seeding rate and, there was no occurrence of green seeds.

  9. Wheat and barley seed systems in Ethiopia and Syria

    NARCIS (Netherlands)

    Bishaw, Z.

    2004-01-01

    Keywords: Wheat,Triticumspp., Barley,Hordeumvulgare L., Seed Systems, Formal Seed Sector, Informal Seed Sector, National Seed Program, Seed Source, Seed Selection, Seed Management, Seed Quality,

  10. Detection and correction of patient movement in prostate brachytherapy seed reconstruction

    Science.gov (United States)

    Lam, Steve T.; Cho, Paul S.; Marks, Robert J., II; Narayanan, Sreeram

    2005-05-01

    Intraoperative dosimetry of prostate brachytherapy can help optimize the dose distribution and potentially improve clinical outcome. Evaluation of dose distribution during the seed implant procedure requires the knowledge of 3D seed coordinates. Fluoroscopy-based seed localization is a viable option. From three x-ray projections obtained at different gantry angles, 3D seed positions can be determined. However, when local anaesthesia is used for prostate brachytherapy, the patient movement during fluoroscopy image capture becomes a practical problem. If uncorrected, the errors introduced by patient motion between image captures would cause seed mismatches. Subsequently, the seed reconstruction algorithm would either fail to reconstruct or yield erroneous results. We have developed an algorithm that permits detection and correction of patient movement that may occur between fluoroscopy image captures. The patient movement is decomposed into translational shifts along the tabletop and rotation about an axis perpendicular to the tabletop. The property of spatial invariance of the co-planar imaging geometry is used for lateral movement correction. Cranio-caudal movement is corrected by analysing the perspective invariance along the x-ray axis. Rotation is estimated by an iterative method. The method can detect and correct for the range of patient movement commonly seen in the clinical environment. The algorithm has been implemented for routine clinical use as the preprocessing step for seed reconstruction.

  11. VIGOR TESTS ASSOCIATION AS AN ALTERNATIVE FOR PRECISE AND EFFICIENT ASSESSMENT OF MAIZE SEED QUALITY

    Directory of Open Access Journals (Sweden)

    MARCOS ALTOMANI NEVES DIAS

    2015-01-01

    Full Text Available This study aimed to associate two concepts of seed vigor testing, the aging and seedling growth using image analysis, providing a feasible and time-saving way to evaluate maize seed vigor in large scale. For this purpose, five seed lots with different vigor levels from two single hybrids were used. The seeds were characterized by moisture content, germination, seedling emergence, seedling emergence speed index, accelerated aging and cold tests. The treatments were composed by varying the accelerated aging periods (0, 24, 48, 72 and 96h and seedling growth periods (48 and 72h, before submitting the seedlings to image analysis for seedling root length measurement. Considering the results obtained, the adaptation of accelerated aging test by reducing the aging duration from 96h to 48h, and the replacement of the germination test by seedling root length measurement using image analysis could be considered a potential tool for maize seed vigor assessment. The treatments composed of 48h of seed aging followed by seedlings root length measurements using image analysis provided reliable data, compared to traditional vigor tests and it could be considered an efficient and timesaving approach, associating two different concepts of seed vigor analysis.

  12. A system for generating virtual seeds

    Directory of Open Access Journals (Sweden)

    Sako Y.

    1998-01-01

    Full Text Available Seed analysts need to identify seeds, and seed catalogs are used as a reference to accomplish this task. Conventional seed catalogs supply two-dimensional photographs and hand-drawn diagrams. In this study, a new, three-dimensional representation of seeds is developed to supplement these traditional photographs and drawings. QuickTime VR is a promising method for viewing three-dimensional objects on a computer screen. It permits manipulation of an object by rotating and viewing it from any pre-specified angle at an interactive speed, allowing the viewer the sense of examining a hand-held object. In this study, QuickTime VR object movies of seeds were created as interactive "movies" of seeds that can be rotated and scaled to give the viewer the sensation of examining actual seeds. This approach allows the examination of virtual seeds from any angle, permitting more accurate identification of seeds by seed analysts.

  13. Visualizing tissue molecular structure of a black type of canola (Brassica) seed with a thick seed coat after heat-related processing in a chemical way.

    Science.gov (United States)

    Yu, Peiqiang

    2013-02-20

    Heat-related processing of cereal grains, legume seeds, and oil seeds could be used to improve nutrient availability in ruminants. However, different types of processing may have a different impact on intrinsic structure of tissues. To date, there is little research on structure changes after processing within intact tissues. The synchrotron-based molecular imaging technique enables us to detect inherent structure change on a molecular level. The objective of this study was to visualize tissue of black-type canola (Brassica) seed with a thick seed coat after heat-related processing in a chemical way using the synchrotron imaging technique. The results showed that the chemical images of protein amides were obtained through the imaging technique for the raw, wet, and dry heated black type of canola seed tissues. It seems that different types of processing have a different impact on the protein spectral profile in the black type of canola tissues. Wet heating had a greater impact on the protein α-helix to β-sheet ratio than dry heating. Both dry and wet heating resulted in different patterns in amide I, the second derivative, and FSD spectra. However, the exact differences in the tissue images are relatively difficult to be obtained through visual comparison. Future studies should focus on (1) comparing the response and sensitivity of canola seeds to various processing methods between the yellow-type and black-type of canola seeds; (2) developing a sensitive method to compare the image difference between tissues and between treatments; (3) developing a method to link images to nutrient digestion, and (4) revealing how structure changes affect nutrient absorption in humans and animals.

  14. Suggestions on technical guide of implantation of radioactive seeds

    International Nuclear Information System (INIS)

    Wang Zhongmin; Huang Gang; Lv Zhongwei; Liu Jianjun; Chen Kemin; Chen Yongde

    2009-01-01

    Implantation of radioactive seeds is an effective therapeutic option for the treatment of malignant tumors. With the development of imaging technique and the use of treatment planning system (TPS) it has been more and more employed in clinical settings. The technique has been widely practiced in various malignant tumors, such as prostate cancer, lung caner, pancreatic cancer, hepatocarcinoma, etc. In order to standardize the clinical application of this technology, the authors propose some suggestions concerning the management of radioactive seeds, the indications and contraindications as well as the method of operation as a technical guidance. (authors)

  15. Suggestions on technical guide of implantation of radioactive seeds

    Energy Technology Data Exchange (ETDEWEB)

    Zhongmin, Wang; Gang, Huang; Zhongwei, Lv; Jianjun, Liu [Department of Radiology, Luwan Branch of Ruijin Hospital, School of Medicine, Shanghai Jiaotong Univ., Shanghai (China); Kemin, Chen; Yongde, Chen

    2009-09-15

    Implantation of radioactive seeds is an effective therapeutic option for the treatment of malignant tumors. With the development of imaging technique and the use of treatment planning system (TPS) it has been more and more employed in clinical settings. The technique has been widely practiced in various malignant tumors, such as prostate cancer, lung caner, pancreatic cancer, hepatocarcinoma, etc. In order to standardize the clinical application of this technology, the authors propose some suggestions concerning the management of radioactive seeds, the indications and contraindications as well as the method of operation as a technical guidance. (authors)

  16. Qualidade fisiológica de sementes de pitangueira submetidas a diferentes procedimentos de secagem e substratos - Parte 2 Physiological quality of surinam cherry seeds submitted to different procedures of drying and substrates - Part 2

    Directory of Open Access Journals (Sweden)

    Lúcia H. de M. Sena

    2010-04-01

    Full Text Available Conduziu-se este trabalho com o objetivo de determinar o procedimento de secagem e substratos ideais para avaliar a visibilidade e o vigor das sementes e crescimento inicial das plântulas de pitangueira (Eugenia uniflora L.. Utilizou-se o delineamento inteiramente casualizado, em esquema fatorial 2 x 4 (2 procedimentos de secagem: à sombra e ao sol; 4 substratos: Areia, vermiculita, pó-de-coco e papel toalha, com quatro repetições de 25 sementes cada uma e se avaliaram os seguintes parâmetros: tempo médio de germinação, comprimento da raiz e do epicótilo e massa seca da plântula. O experimento foi conduzido em germinador tipo BOD, regulado a temperatura constante de 25 ºC e regime de luz contínua. O procedimento de secagem à sombra e o substrato vermiculita podem ser recomendados para testar o vigor dessa espécie.This study had as its objective the determination of ideal procedure of drying and the substrate to evaluate the vigour and the initial growth of surinam cherry seedlings (Eugenia uniflora L.. The used design was entirely randomized in factorial scheme 2 x 4 (2 procedures of drying: shade and sun; 4 substrates: sand, vermiculite, coconut fiber and paper towels with four replications of 25 seeds each. The following parameters were also analyzed: average time of germination, length of primary root and epicotyl and dry weight of the seedling. The experiment was conducted in BOD regulated at constant temperature of 25 ºC and regime of continuous light. The shade method of drying and the vermiculite substrate can be recommended to test the vigour of this specie.

  17. Seed selection by earthworms : chemical seed properties matter more than morphological traits

    OpenAIRE

    Clause, J.; Forey, E.; Eisenhauer, N.; Seal, C.E.; Soudey, A.; Colville, L.; Barot, Sébastien

    2017-01-01

    Aims : The passage of seeds through the earthworm gut potentially damages seeds, altering seed and seedling performances depending on seed traits. This work was conducted to study to what extent chemical and morphological seed traits determine the seed attractiveness for earthworms. Methods : We tested seed selection via the ingestion and digestion of 23 grassland plant species spanning a range of 14 morphological and chemical traits by two common earthworm species: the anecic Lumbricus te...

  18. Effect of seed treatment with static magnetic field (SMF) and low dose gamma radiation (GR) on grain yield of aerobic rice

    International Nuclear Information System (INIS)

    Kumar, Dinesh; Anand, Anjali; Singh, Bhupinder

    2014-01-01

    Aerobic rice cultivation is gaining popularity as it demands less water. However, poor germination of rice is an important issue in this situation. Seed pretreatment with static magnetic field (SMF) and gamma radiation (GR) at prescribed dose is known to influence the germination, seedling vigour and and yield of many crops. There is a possibility to improve the crop establishment under aerobic situation by physical seed treatment with static-magnetic field (SMF) and gamma radiation (GR) prior to sowing. Hence, a field experiment was conducted at the Indian Agricultural Research Institute, New Delhi during kharif 2012 and 2013 to study the effect of SMF and GR-treated seeds on growth and yield of aerobic rice. The five seed treatments were: SMF 50 mT for 2 hrs, SMF 100 mT for 2 hrs, GR 0.0025 kGy, GR 0.10 kGy and an untreated control. The experiment was laid out in a Randomized Block Design with four replications. Crop (variety 'Pusa Basmati-1121') was direct seeded on 25 th and 24 th June during 2012 and 2013, respectively at a spacing of 25 cm. Treatments GR 0.0025 kGy, SMF (50 mT) and SMF (100 mT) resulted in a significant improvement in grain yield of rice over control and GR dose (0.10 kGy) during both the years. Averaged across two years the grain yield increase by treating the rice seeds with GR 0.0025 kGy, SMF (50 mT) and SMF (100 mT) was 20.1, 17.6 and 14.5%, respectively over the control. Increase in GR dose (0.10 kGy ) was not effective in improving the yield, and was found to be similar to control. It is therefore concluded that treatment of rice seeds either with GR (0.0025 kGy) or SMF (50 mT) holds a great promise in increasing the grain yield of aerobic rice. (author)

  19. Effects of seed fermentation method on seed germination and vigor ...

    African Journals Online (AJOL)

    BERTIN

    2013-11-27

    Nov 27, 2013 ... methods (fermented in ambient air, plastic bag stored in ambient or in plastic bag buried) were tested ... fruits into plastic bag that was exposed in ambient air in the field; SFD, seeds ..... Concepts and technologies of selected.

  20. Improving the traditional sesame seed planting with seed pelleting

    African Journals Online (AJOL)

    PRECIOUS

    2009-11-16

    Nov 16, 2009 ... 1Department of Agricultural Machinery, Faculty of Agriculture, Adnan Menderes University, Turkey. 2Fethiye ... the pelleted sesame seeds improved the yield significantly ... Sesame holds a special importance in the world's oil.

  1. Laser treatment of radish seed

    International Nuclear Information System (INIS)

    Kartalov, P.; Nidal, T.

    1987-01-01

    Trials were conducted in unheated plastic greenhouses in 1985-1986 to test the effect of laser treatment on radish seed. Seed of cv Saxia was irradiated with helio-neon laser of 632.8 Nm wave length at: 2-, 4- and 6-fold irradiation. Results showed that plants of all variants emerged almost simultaneously. The root mass was greatest for plants obtained from 4-fold irradiated seed. Treatment enhanced root production in 1985, and in 1986 4-fold irradiation boosted yield by 15%

  2. Seed cryopreservation of Fraxinus angustifolia Vahl

    African Journals Online (AJOL)

    Università

    2013-04-17

    control) and liquid nitrogen-treated seeds were .... 76.0 ± 4.0 (NT in GA3), with an intermediary value of 70.0. ± 5.0% (Cryo seeds in water .... in November, suggesting a loss of dormancy during seed maturation. Furthermore, seed ...

  3. Storage requirements for sugar maple seeds

    Science.gov (United States)

    Harry W. Yawney; Clayton M., Jr. Carl

    1974-01-01

    Sugar maple seeds, collected from three trees in northern Vermont, were stored at four temperatures (18, 7, 2, and -10ºC) in combination with four seed moisture contents (35, 25, 17, and 10 percent). Seed moisture content and storage temperature significantly affected keeping ability, and these factors were highly interrelated. Seeds from all trees kept best...

  4. 7 CFR 948.6 - Seed potatoes.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Seed potatoes. 948.6 Section 948.6 Agriculture... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE IRISH POTATOES GROWN IN COLORADO Order Regulating Handling Definitions § 948.6 Seed potatoes. Seed potatoes or seed means any potatoes...

  5. 7 CFR 201.57 - Hard seeds.

    Science.gov (United States)

    2010-01-01

    ... REGULATIONS Germination Tests in the Administration of the Act § 201.57 Hard seeds. Seeds which remain hard at the end of the prescribed test because they have not absorbed water, due to an impermeable seed coat... percentage of germination. For flatpea, continue the swollen seed in test for 14 days when germinating at 15...

  6. Brassica rapa L. seed development in hypergravity

    NARCIS (Netherlands)

    Musgrave, M.E.; Kuang, A.; Allen, J.; Blasiak, J.; van Loon, J.J.W.A.

    2009-01-01

    Previous experiments had shown that microgravity adversely affected seed development in Brassica rapa L. We tested the hypothesis that gravity controls seed development via modulation of gases around the developing seeds, by studying how hypergravity affects the silique microenvironment and seed

  7. Evaluation of Lettuce Genotypes for Seed Thermotolerance

    Science.gov (United States)

    Thermoinhibition of lettuce (Lactuca sativa L.) seed germination is a common problem associated with lettuce production. Depending on lettuce cultivars, seed germination may be inhibited when temperatures exceed 28oC. The delay or inhibition of seed germination at high temperatures may reduce seedli...

  8. Analysis of seed quality in NS sunflower hybrid seed processed between 2010 and 2014

    Directory of Open Access Journals (Sweden)

    Jokić Goran

    2015-01-01

    Full Text Available This paper analyzed the processed seed of five sunflower hybrid seed developed at the Institute of Field and Vegetable Crops in Novi Sad. The cultivars were Rimi PR, Duško, NS Dukat, Sumo 1 PR and Sremac. The analysis was conducted on seed lots processed between 2010 and 2014 and involved the following parameters: seed purity percentage, 1000-seed weight, germination energy, germination, seed moisture, number of weed seeds per 1000 grams of seed. The results of the study produced the following average values: seed purity - 99.72%, 1000-seed weight - 67.59g, germination energy - 88.2%, germination - 91.8%, seed moisture - 8.3%. There were not found weeds seeds as well as pathogens on the seed samples, these values are all within the legally prescribed limits.

  9. Autoradiography for iodine-125 seeds

    International Nuclear Information System (INIS)

    Alberti, W.; Divoux, S.; Pothmann, B.; Tabor, P.; Hermann, K.P.; Harder, D.

    1993-01-01

    To study the interior design of model 6702 and 6711 iodine-125 seeds, contact autoradiographs were performed using mammography film. Improved resolution was obtained using a pin-hole camera with a hole of 0.1 mm x 0.1 mm. With these techniques, qualitative determination of the relative activity distribution within each seed was possible. The number of the activated resin spheres and the positions of the centers of these spheres can be exactly determined. A model calculation shows that variations in the arrangement of the activated spheres within a seed have a moderate influence on the dose distribution at source distances below 10 mm. Knowing the exact source configuration may be useful when comparing dose calculations with measured data for model 6702 125 I seeds which are currently employed in ophthalmic plaque and implant therapy of other tumors. 16 refs., 5 figs., 2 tabs

  10. Ethylene, seed germination, and epinasty.

    Science.gov (United States)

    Stewart, E R; Freebairn, H T

    1969-07-01

    Ethylene activity in lettuce seed (Lactuca satina) germination and tomato (Lycopersicon esculentum) petiole epinasty has been characterized by using heat to inhibit ethylene synthesis. This procedure enabled a separation of the production of ethylene from the effect of ethylene. Ethylene was required in tomato petioles to produce the epinastic response and auxin was found to be active in producing epinasty through a stimulation of ethylene synthesis with the resulting ethylene being responsible for the epinasty. In the same manner, it was shown that gibberellic acid stimulated ethylene synthesis in lettuce seeds. The ethylene produced then in turn stimulated the seeds to germinate. It was hypothesized that ethylene was the intermediate which caused epinasty or seed germination. Auxin and gibberellin primarily induced their response by stimulating ethylene production.

  11. Lack of caching of direct-seeded Douglas fir seeds by deer mice

    International Nuclear Information System (INIS)

    Sullivan, T.P.

    1978-01-01

    Seed caching by deer mice was investigated by radiotagging seeds in forest and clear-cut areas in coastal British Columbia. Deer mice tend to cache very few Douglas fir seeds in the fall when the seed is uniformly distributed and is at densities comparable with those used in direct-seeding programs. (author)

  12. Seed mass and mast seeding enhance dispersal by a neotropical scatter-hoarding rodent

    NARCIS (Netherlands)

    Jansen, P.A.; Bongers, F.J.J.M.; Hemerik, L.

    2004-01-01

    Many tree species that depend on scatter-hoarding animals for seed dispersal produce massive crops of large seeds at irregular intervals. Mast seeding and large seed size in these species have been explained as adaptations to increase animal dispersal and reduce predation. We studied how seed size

  13. Chloroxyanion residue on seeds and sprouts after chlorine dioxide sanitation of alfalfa seed

    Science.gov (United States)

    The effects of a 6-h chlorine dioxide sanitation of alfalfa seed (0, 50, 100, and 200 mg/kg seed) on total coliform bacteria, seed germination, and on the presence of chlorate and perchlorate residues in seed rinse, seed soak, and in alfalfa sprouts was determined. Chlorate residues in 20000 ppm cal...

  14. Moringa Seed Oils

    Directory of Open Access Journals (Sweden)

    Joana O. Ilesanmi

    2010-01-01

    Full Text Available This study was conducted to determine the effects of neem (Azadirachta indica A. Juss and moringa (Moringa oleifera seed oils on the storability of cowpea grain. Cowpea samples were treated with various concentrations (0.5, 1.0, and 1.5 mL/200 g cowpea of pure neem and moringa oils and their mixtures in ratios of 1:1, 1:2, and 1:3. The treated cowpea samples were stored for 180 days. Data were collected every 30 days on number of eggs laid, total weevil population, and percentage of uninfested grains and analysed statistically. Significantly different means were compared using LSD at <.05. Increasing oil concentration resulted in better cowpea protection, for example, in oviposition where the control had 6513 eggs, only 8 eggs were recorded in pure neem oil-treated sample at 0.5 mL/200 g. Generally, better results were obtained with higher oil concentrations either in their pure forms or mixtures. The control had a total weevil population of 4988, while most treated samples had none. The control samples had 0% uninfested grains, while 73–94% of uninfested grains were observed in treated samples after 6 months of storage. Therefore, mixture of the oils at 1.5 mL/200 g can be effectively used to store cowpea.

  15. Diamond Synthesis Employing Nanoparticle Seeds

    Science.gov (United States)

    Uppireddi, Kishore (Inventor); Morell, Gerardo (Inventor); Weiner, Brad R. (Inventor)

    2014-01-01

    Iron nanoparticles were employed to induce the synthesis of diamond on molybdenum, silicon, and quartz substrates. Diamond films were grown using conventional conditions for diamond synthesis by hot filament chemical vapor deposition, except that dispersed iron oxide nanoparticles replaced the seeding. This approach to diamond induction can be combined with dip pen nanolithography for the selective deposition of diamond and diamond patterning while avoiding surface damage associated to diamond-seeding methods.

  16. Experimental dosimetry of Ho-166 bioglass seed polymer-protected

    Energy Technology Data Exchange (ETDEWEB)

    Nogueira, Luciana B.; Campos, Tarcisio P.R. [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Dept. de Engenharia Nuclear. Programa de Pos-Graduacao em Ciencias e Tecnicas Nucleares

    2011-07-01

    This study aims to develop experimental dosimetry of Ho-166 bio glass seed for brachytherapy studies using GAFCHROMIC EBT2 radio chromium films. The methodology consists of placement of radio chromium films in a compressed breast phantom, along with bio glass polymer-protected seeds of [Si: Ca: Ho] and [Si: Ca: Ho: Zr]. The bio glass seeds were encapsulated with polyvinyl alcohol, before being activated and used in the study. The bio glass seeds were introduced into the breast phantom, along with radio chromium films for a period of 2 hours. After the exposure time, radio chromium films were removed from phantom and digitized for analysis in ImageDIG 2.0 program, which quantifies the intensity of RGB (Red, Green, Blue). The dose calculation was evaluated by Monte Carlo technique. Experimental and theoretical data were used to calibrate the dose distribution. The results were plotted on graphs and dose iso curves were obtained. As conclusion it is possible to perform dosimetry in Ho-166 seed brachytherapy using radio chromium films, limited to a short exposure time and small activity. (author)

  17. Experimental dosimetry of Ho-166 bioglass seed polymer-protected

    International Nuclear Information System (INIS)

    Nogueira, Luciana B.; Campos, Tarcisio P.R.

    2011-01-01

    This study aims to develop experimental dosimetry of Ho-166 bio glass seed for brachytherapy studies using GAFCHROMIC EBT2 radio chromium films. The methodology consists of placement of radio chromium films in a compressed breast phantom, along with bio glass polymer-protected seeds of [Si: Ca: Ho] and [Si: Ca: Ho: Zr]. The bio glass seeds were encapsulated with polyvinyl alcohol, before being activated and used in the study. The bio glass seeds were introduced into the breast phantom, along with radio chromium films for a period of 2 hours. After the exposure time, radio chromium films were removed from phantom and digitized for analysis in ImageDIG 2.0 program, which quantifies the intensity of RGB (Red, Green, Blue). The dose calculation was evaluated by Monte Carlo technique. Experimental and theoretical data were used to calibrate the dose distribution. The results were plotted on graphs and dose iso curves were obtained. As conclusion it is possible to perform dosimetry in Ho-166 seed brachytherapy using radio chromium films, limited to a short exposure time and small activity. (author)

  18. Qualidade fisiológica de sementes de pitangueira submetidas a diferentes procedimentos de secagem e substratos - Parte 1 Physiological quality of surinam cherry seeds submitted to different procedures of drying and substrates - Part 1

    Directory of Open Access Journals (Sweden)

    Lúcia H. de M. Sena

    2010-04-01

    Full Text Available Propôs-se, no presente trabalho, determinar o procedimento de secagem e substratos ideais para avaliar a viabilidade e o vigor de sementes de pitangueira (Eugenia uniflora L.. O delineamento experimental adotado foi o inteiramente casualizado, em arranjo fatorial 2 x 4 (2 procedimentos de secagem: à sombra e ao sol; 4 substratos: Areia, vermiculita, pó-de-coco e papel toalha com quatro repetições de 25 sementes cada uma. Caracterizou-se a curva de absorção de água pela semente e se avaliaram os seguintes parâmetros: germinação (%, primeira contagem de germinação (%, índice de velocidade de germinação e sementes mortas (%. O experimento foi conduzido em germinador do tipo BOD, regulado a temperatura constante de 25 ºC e regime de luz contínua. Nas condições em que o trabalho foi realizado, a secagem à sombra e o substrato vermiculita podem ser recomendados para avaliar a qualidade fisiológica, porque proporcionaram maior germinação e desenvolvimento inicial das plântulas de pitangueira.The present work had as its objective to determine the ideal procedure of drying and the substrate to evaluate the viability and vigour of surinam cherry seeds (Eugenia uniflora L.. The experiment was developed in a completely randomized design in factorial scheme 2 x 4 (2 procedures of drying: shade and sun; 4 substrates: sand, vermiculite, coconut fiber and paper towels with four replications of 25 seeds each. The water absortion curve was characterized and the following parameters were analyzed: germination percentage, first germination count (%, germination speed index and dead seeds (%. The experiment was conducted in a BOD germinator regulated at constant temperature of 25 ºC and regime of continuous light. In the conditions where the work was carried out, the drying under shade and vermiculite may be recommended to evaluate the vigour because they provided the best germination and initial development of surinam cherry seedlings.

  19. Seed governance. From seed aid to seed system security in fragile areas

    NARCIS (Netherlands)

    Rietberg, P.I.; Gevers, H.; Hospes, O.

    2014-01-01

    Intergovernmental agencies and development organizations, including Cordaid, consider interventions directed at seed security of utmost importance to support smallholders recovering from conflict situations and disasters, and to contribute to revitalisation of local agricultural production and food

  20. Seed loss in prostate brachytherapy. Operator dependency and impact on dosimetry

    Energy Technology Data Exchange (ETDEWEB)

    El-Bared, Nancy; Sebbag, Natanel; Beliveau-Nadeau, Dominic; Hervieux, Yannick; Larouche, Renee; Taussky, Daniel; Delouya, Guila [Centre hospitalier de l' Universite de Montreal - Hopital Notre-Dame, Departement de Radio-Oncologie, Montreal, QC (Canada)

    2016-05-15

    The aim of our study was to review seed loss and its impact on dosimetry as well as the influence of the treating physician on seed loss and dosimetry in patients treated with prostate brachytherapy using permanent loose {sup 125}I implant. We analyzed 1087 consecutive patients treated by two physicians between July 2005 and April 2015 at a single institution. Pelvic fluoroscopic imaging was done 30 days post implant and a chest X-ray when seed loss was observed. Seed loss occurred in 19.4 % of patients: in 20.0 % of implants done by the most experienced physician and in 17.2 % by the less experienced physician (p = 0.4) and migration to the thorax occurred in 5.9 % (6.9 vs. 2.2 %, p = 0.004). The mean seed loss rate was 0.57 % [standard deviation (SD) 1.39] and the mean rate of seeds in the thorax was 0.14 % (SD 0.65). The most experienced physician had a higher mean number of seeds lost: 0.36 versus 0.25 (p = 0.055), and a higher mean number of seed migration to the thorax: 0.1 versus 0.02 (p < 0.001). When at least one seed was lost, a decrease of 4.2 Gy (p < 0.001) in the D90 and a decrease of 3.5 % (p = 0.002) in the V150 was observed. We found a significant decrease in V150 and D90 with the occurrence of seed loss. Furthermore, we found a difference in seed migration among the physicians demonstrating that seed loss is operator dependant. (orig.) [German] Wir analysierten den Prozentsatz des Seed-Verlusts sowie den Einfluss von Arzterfahrung und Seed-Abgang auf die Dosimetrie bei Patienten, die mit einer Prostata-Brachytherapie mit permanent beweglichen {sup 125}I-Implantaten behandelt wurden. Eingeschlossen in diese Studie wurden alle zwischen Juli 2005 und April 2015 an unserem Krankenhaus von zwei Aerzten konsekutiv behandelten 1087 Patienten. Anhand fluoroskopischer Bilder wurden noch vorhandene Seeds 30 Tage nach dem Eingriff gezaehlt. Bei unvollstaendiger Seed-Anzahl wurde ein Thorax-Roentgenbild angefertigt. In 19% der Patienten ging mindestens ein

  1. Phenolics in the seed coat of wild soybean (Glycine soja) and their significance for seed hardness and seed germination.

    Science.gov (United States)

    Zhou, San; Sekizaki, Haruo; Yang, Zhihong; Sawa, Satoko; Pan, Jun

    2010-10-27

    Hardseededness in annual wild soybean (Glycine soja Sieb. Et Zucc.) is a valuable trait that affects the germination, viability, and quality of stored seeds. Two G. soja ecotypes native to Shandong Province of China have been used to identify the phenolics in the seed coat that correlate with the seed hardness and seed germination. Three major phenolics from the seed coat were isolated and identified as epicatechin, cyanidin 3-O-glucoside, and delphinidin 3-O-glucoside. Of the three phenolics, only the change of epicatechin exhibited a significant positive correlation with the change of hard seed percentages both under different water conditions during seed development and under different gas conditions during seed storage. Epicatechin also reveals a hormesis-like effect on the seed germination of G. soja. Epicatechin is suggested to be functionally related to coat-imposed hardseededness in G. soja.

  2. Equilibrium relative humidity as a tool to monitor seed moisture

    Science.gov (United States)

    Robert P. Karrfalt

    2010-01-01

    The importance of seed moisture in maintaining high seed viability is well known. The seed storage chapters in the Tropical Tree Seed Manual (Hong and Ellis 2003) and the Woody Plant Seed Manual (Bonner 2008a) give a detailed discussion and many references on this point. Working with seeds in an operational setting requires a test of seed moisture status. It is...

  3. Anatomy guided automated SPECT renal seed point estimation

    Science.gov (United States)

    Dwivedi, Shekhar; Kumar, Sailendra

    2010-04-01

    Quantification of SPECT(Single Photon Emission Computed Tomography) images can be more accurate if correct segmentation of region of interest (ROI) is achieved. Segmenting ROI from SPECT images is challenging due to poor image resolution. SPECT is utilized to study the kidney function, though the challenge involved is to accurately locate the kidneys and bladder for analysis. This paper presents an automated method for generating seed point location of both kidneys using anatomical location of kidneys and bladder. The motivation for this work is based on the premise that the anatomical location of the bladder relative to the kidneys will not differ much. A model is generated based on manual segmentation of the bladder and both the kidneys on 10 patient datasets (including sum and max images). Centroid is estimated for manually segmented bladder and kidneys. Relatively easier bladder segmentation is followed by feeding bladder centroid coordinates into the model to generate seed point for kidneys. Percentage error observed in centroid coordinates of organs from ground truth to estimated values from our approach are acceptable. Percentage error of approximately 1%, 6% and 2% is observed in X coordinates and approximately 2%, 5% and 8% is observed in Y coordinates of bladder, left kidney and right kidney respectively. Using a regression model and the location of the bladder, the ROI generation for kidneys is facilitated. The model based seed point estimation will enhance the robustness of kidney ROI estimation for noisy cases.

  4. The Seed Proteome Web Portal

    Directory of Open Access Journals (Sweden)

    Marc eGalland

    2012-06-01

    Full Text Available The Seed Proteome Web Portal (SPWP; http://www.seedproteome.com/ gives access to information both on quantitative seed proteomic data and on seed-related protocols. Firstly, the SPWP provides access to the 475 different Arabidopsis seed proteins annotated from 2 dimensional electrophoresis (2DE maps. Quantitative data are available for each protein according to their accumulation profile during the germination process. These proteins can be retrieved either in list format or directly on scanned 2DE maps. These proteomic data reveal that 40% of seed proteins maintain a stable abundance over germination, up to radicle protrusion. During sensu stricto germination (24 h upon imbibition about 50% of the proteins display quantitative variations, exhibiting an increased abundance (35% or a decreasing abundance (15%. Moreover, during radicle protrusion (24 h to 48 h upon imbibition, 41% proteins display quantitative variations with an increased (23% or a decreasing abundance (18%. In addition, an analysis of the seed proteome revealed the importance of protein post-translational modifications as demonstrated by the poor correlation (r2 = 0.29 between the theoretical (predicted from Arabidopsis genome and the observed protein isoelectric points. Secondly, the SPWP is a relevant technical resource for protocols specifically dedicated to Arabidopsis seed proteome studies. Concerning 2D electrophoresis, the user can find efficient procedures for sample preparation, electrophoresis coupled with gel analysis and protein identification by mass spectrometry, which we have routinely used during the last 12 years. Particular applications such as the detection of oxidized proteins or de novo synthetized proteins radiolabeled by [35S]-methionine are also given in great details. Future developments of this portal will include proteomic data from studies such as dormancy release and protein turnover through de novo protein synthesis analyses during germination.

  5. Report on seed born diseases in organic seed and propagation material

    OpenAIRE

    Micheloni, C.; Plakolm, G.; Schärer, H.

    2007-01-01

    The key questions which will be addressed in this report are: • Are seed born diseases an important factor that prevents seed companies from producing organic seeds and organic farmers from using them? • Which seed treatments are available in organic farming? Which treatments are or will be acceptable? To which degree are they effective? • Are the thresholds for seed born diseases different among Member States? Can this cause unfair competition among farmers and seed producers? • ...

  6. Physiological quality of vegetable soybean seeds produced with different fertilization and storage for twelve months = Potencial fisiológico de sementes de soja-hortaliça produzidas com diferentes adubações e armazenadas por doze meses

    Directory of Open Access Journals (Sweden)

    Juliana Maria Espíndola Lima

    2013-04-01

    Full Text Available Abstract - The vegetable soybean (Glycine max (L. Merrill has characteristics that permit utilization in food as a vegetablewhen the seeds are still immature (stage R6 and occupy 80-90% of the cavity filling the pod. The objective of this study was to evaluate the physiological potential of seeds of two vegetable soybean genotypes produced with different fertilization and stored for 12 months. In seed production field was used in the experimental design of randomized blocks with 5x2 factorial, five fertilization (conventional, intermediate, alternative, manipueira and rice hulls and two periods of evaluation (at 12 months of storage. Evaluations conducted under laboratory conditions included mass of a hundred seeds, vigor and physiological quality considering germination, germination first count, electrical conductivity, potassium leaching, sand emergence and emergence rate of seedlings. The seeds of both vegetable soybean genotype were stored in polyethylene terephthalate bottles for 12 months, as to assess physical and physiological quality under storage. Seeds of BR9452273 produced with carbonized ricehusk showed better quality at harvest and presented greater reduced of vigour after 12 months of storage. BRS 258 cultivar conventional fertilization showed seeds of less physiological quality and higher reduced the vigour after 12 months of storage. The two vegetable soybean cultivars have quality reduced during storage of seed of twelve months in bottles of polyethylene terephthalate. = Resumo – A soja-hortaliça (Glycine max (L. Merrill apresenta características que permitem utilização na alimentação humana como hortaliça, quando as sementes estão ainda imaturas (estádio R6 e ocupam 80 a 90% do preenchimento da cavidade da vagem. Objetivou-se com este trabalho avaliar o potencial fisiológico das sementes de dois genótipos de soja-hortaliça produzidas com diferentes adubações e armazenadas por 12 meses. O delineamento

  7. Envelhecimento acelerado e deterioração controlada em sementes de amendoim Accelerated aging and controlled deterioration of peanut seeds

    Directory of Open Access Journals (Sweden)

    Claudia Antonia Vieira Rossetto

    2004-08-01

    Full Text Available Pesquisas têm sido desenvolvidas visando determinar os procedimentos para a avaliação de diferenças no potencial fisiológico de lotes de sementes, com destaque aos testes de vigor. O objetivo deste trabalho foi estudar o envelhecimento acelerado e a deterioração controlada para avaliação do vigor de sementes de amendoim (Arachis hypogaea. Quatro lotes de sementes de amendoim da cultivar Tatu foram submetidos ao teste de envelhecimento acelerado, em caixas tipo gerbox, com temperatura de 42ºC ou 43ºC, por 48 horas e 72 horas, com e sem o emprego de solução saturada de NaCl, e ao teste de deterioração controlada, com teor de água de 15% e 20%, a 40ºC ou 45ºC, por 48 horas. O teor de água, a germinação e sanidade das sementes foram determinados. Pelos testes de germinação e de emergência, não houve diferença significativa de desempenho entre os quatro lotes. No teste de envelhecimento acelerado com solução salina, o período de 72 horas a 42ºC é suficiente para avaliar o potencial fisiológico das sementes. No teste de deterioração controlada, a combinação de 15% de teor de água nas sementes e 48 horas em banho maria a 45ºC é eficiente para detectar diferenças de vigor entre os lotes.Researches have been developed aiming to determine the procedures for physiological potential seeds evaluation with emphasis to vigour tests. The objective was to study the controlled deterioration and the accelerated aging in peanut (Arachis hypogaea seeds vigour. Four lots of peanut seeds were submitted to the ageing accelerated test, at 42ºC or 43ºC, for 48 hours and 72 hours, with or without the use of saturated solution of NaCl, and controlled deterioration test, with 15% and 20% water content, at 40ºC or 45ºC, for 48 hours. The evaluation of water content, germination and health test was accomplished. There was no different performance among the four lots by the germination and emergency tests. In the ageing accelerated

  8. Trafkintu: seed curators defending food sovereignty

    Directory of Open Access Journals (Sweden)

    Nastassja Nicole Mancilla Ivaca

    2014-10-01

    Full Text Available This paper examines the resurgence of Trafkintu, an ancient Mapuche ritual of seed trade; now as a folk-communication practice of resistance, against neoliberal transformations in farming that threaten food sovereignty of rural communities in southern Chile. Drawing onparticipant observation and semi-structured interviews with peasant and Mapuche women involved in these practices, we show that seed curators women act as agents that revalue the localness [lo local] through a process of resignification of Trafkintu, this time linking it tofood self-sufficiency. In addition, they build networks between indigenous and peasant communities as a resistance strategy. However, this resurgence of Trafkintu becomes ambivalent as its new symbolic expression is being appropriated by local mainstreampoliticians, for electoral purposes, to promote an image of 'concern about popular culture'. That is, a tool of resistance, on the one hand, and a kind of political folk-marketing, on the other.

  9. Influence of Fungicides Application and Seed Processing on Sunflower Seed Quality

    OpenAIRE

    Šimić, Branimir; Svitlica, Brankica; Ćosić, Jasenka; Andrić, Luka; Rozman, Vlatka; Postić, Jelena; Liović, Ivica

    2009-01-01

    The aim of the research was to determine influence of methods of seed processing and application of fungicides on mass of 1000 seeds, intergrowth energy and seed germination of sunflower hybrids ‘Favorit’ and ‘Apolon’ in 2005 and 2006. Selected untreated seeds of both sunflower hybrids (control) had a minimal mass of 1000 seeds (55-59 g), intergrowth energy (18-37 %) and seed germination (39-52 %). In both years of research maximal seed quality, mass of 1000 seeds (67-69 g), intergrowth energ...

  10. Neutron irradiation of seeds 2

    Energy Technology Data Exchange (ETDEWEB)

    1968-10-01

    The irradiation of seeds with the fast neutron of research reactors has been hampered by difficulties in accurately measuring dose and in obtaining repeatable and comparable results. Co-ordinated research under an international program organized by the FAO and IAEA has already resulted in significant improvements in methods of exposing seeds in research reactors and in obtaining accurate dosimetry. This has been accomplished by the development of a standard reactor facility for the neutron irradiation of seeds and standard methods for determining fast-neutron dose and the biological response after irradiation. In this program various divisions of the IAEA and the Joint FAO/IAEA Division co-operate with a number of research institutes and reactor centres throughout the world. Results of the preliminary experiments were reported in Technical Reports Series No. 76, ''Neutron Irradiation of Seeds''. This volume contains the proceedings of a meeting of co-operators in the FAO/IAEA Neutron Seed Irradiation Program and other active scientists in this field. The meeting was held in Vienna from 11 to 15 December 1967. Refs, figs and tabs.

  11. Neutron irradiation of seeds 2

    International Nuclear Information System (INIS)

    1968-01-01

    The irradiation of seeds with the fast neutron of research reactors has been hampered by difficulties in accurately measuring dose and in obtaining repeatable and comparable results. Co-ordinated research under an international program organized by the FAO and IAEA has already resulted in significant improvements in methods of exposing seeds in research reactors and in obtaining accurate dosimetry. This has been accomplished by the development of a standard reactor facility for the neutron irradiation of seeds and standard methods for determining fast-neutron dose and the biological response after irradiation. In this program various divisions of the IAEA and the Joint FAO/IAEA Division co-operate with a number of research institutes and reactor centres throughout the world. Results of the preliminary experiments were reported in Technical Reports Series No. 76, ''Neutron Irradiation of Seeds''. This volume contains the proceedings of a meeting of co-operators in the FAO/IAEA Neutron Seed Irradiation Program and other active scientists in this field. The meeting was held in Vienna from 11 to 15 December 1967. Refs, figs and tabs

  12. Effect of Gold Marker Seeds on Magnetic Resonance Spectroscopy of the Prostate

    Energy Technology Data Exchange (ETDEWEB)

    Hossain, Murshed, E-mail: Murshed.Hossain@fccc.edu [Department of Radiation Oncology, Fox Chase Cancer Center, Philadelphia, PA (United States); Schirmer, Timo [Global MR Applied Science Laboratory, GE Healthcare, Munich (Germany); Richardson, Theresa; Chen, Lili; Buyyounouski, Mark K.; Ma Changming [Department of Radiation Oncology, Fox Chase Cancer Center, Philadelphia, PA (United States)

    2012-05-01

    Purpose: Magnetic resonance stereoscopic imaging (MRSI) of the prostate is an emerging technique that may enhance targeting and assessment in radiotherapy. Current practices in radiotherapy invariably involve image guidance. Gold seed fiducial markers are often used to perform daily prostate localization. If MRSI is to be used in targeting prostate cancer and therapy assessment, the impact of gold seeds on MRSI must be investigated. The purpose of this study was to quantify the effects of gold seeds on the quality of MRSI data acquired in phantom experiments. Methods and Materials: A cylindrical plastic phantom with a spherical cavity 10 centimeters in diameter wss filled with water solution containing choline, creatine, and citrate. A gold seed fiducial marker was put near the center of the phantom mounted on a plastic stem. Spectra were acquired at 1.5 Tesla by use of a clinical MRSI sequence. The ratios of choline + creatine to citrate (CC/Ci) were compared in the presence and absence of gold seeds. Spectra in the vicinity of the gold seed were analyzed. Results: The maximum coefficient of variation of CC/Ci induced by the gold seed was found to be 10% in phantom experiments at 1.5 T. Conclusion: MRSI can be used in prostate radiotherapy in the presence of gold seed markers. Gold seeds cause small effects (in the order of the standard deviation) on the ratio of the metabolite's CC/Ci in the phantom study done on a 1.5-T scanner. It is expected that gold seed markers will have similar negligible effect on spectra from prostate patients. The maximum of 10% of variation in CC/Ci found in the phantom study also sets a limit on the threshold accuracy of CC/Ci values for deciding whether the tissue characterized by a local spectrum is considered malignant and whether it is a candidate for local boost in radiotherapy dose.

  13. Effect of Gold Marker Seeds on Magnetic Resonance Spectroscopy of the Prostate

    International Nuclear Information System (INIS)

    Hossain, Murshed; Schirmer, Timo; Richardson, Theresa; Chen, Lili; Buyyounouski, Mark K.; Ma Changming

    2012-01-01

    Purpose: Magnetic resonance stereoscopic imaging (MRSI) of the prostate is an emerging technique that may enhance targeting and assessment in radiotherapy. Current practices in radiotherapy invariably involve image guidance. Gold seed fiducial markers are often used to perform daily prostate localization. If MRSI is to be used in targeting prostate cancer and therapy assessment, the impact of gold seeds on MRSI must be investigated. The purpose of this study was to quantify the effects of gold seeds on the quality of MRSI data acquired in phantom experiments. Methods and Materials: A cylindrical plastic phantom with a spherical cavity 10 centimeters in diameter wss filled with water solution containing choline, creatine, and citrate. A gold seed fiducial marker was put near the center of the phantom mounted on a plastic stem. Spectra were acquired at 1.5 Tesla by use of a clinical MRSI sequence. The ratios of choline + creatine to citrate (CC/Ci) were compared in the presence and absence of gold seeds. Spectra in the vicinity of the gold seed were analyzed. Results: The maximum coefficient of variation of CC/Ci induced by the gold seed was found to be 10% in phantom experiments at 1.5 T. Conclusion: MRSI can be used in prostate radiotherapy in the presence of gold seed markers. Gold seeds cause small effects (in the order of the standard deviation) on the ratio of the metabolite's CC/Ci in the phantom study done on a 1.5-T scanner. It is expected that gold seed markers will have similar negligible effect on spectra from prostate patients. The maximum of 10% of variation in CC/Ci found in the phantom study also sets a limit on the threshold accuracy of CC/Ci values for deciding whether the tissue characterized by a local spectrum is considered malignant and whether it is a candidate for local boost in radiotherapy dose.

  14. Milk line as an indicator of the harvesting time of three hybrid seeds of corn (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Célio Trzeciak dos Santos

    2005-03-01

    Full Text Available The experiment was carried out to evaluate the time for harvesting seeds of three hybrids of corn (Zea mays L. at their best physiological quality and the use of the milk line as an indicator of the physiological seed maturity. Single hybrids CD 1723 and CD 5501 and double hybrid OC 705 seeds were collected for 59 days, every four days, starting 23 days after female flowering. Seed dry weight, moisture content, germination, vigour (cold and accelerated aging tests, black layer formation and seed milk line development were analysed. The harvesting time started 47 days after female flowering with high physiological quality of the seeds identified by a joint analysis of those seven characteristics of them. The milk line at stage 4 proved to be the best indicator of the time to harvest corn seeds for maximum physiological quality, because different stages of milk line development in corn seeds could be easily identified in the filed without any special equipment.O experimento foi conduzido durante o ano agrícola de 1996/97 em área experimental da Cooperativa de Desenvolvimento Econômico e Tecnológico (Coodetec, em Cascavel, Paraná, com o objetivo de avaliar o efeito da época de colheita de três híbridos de milho (Zea mays L. na qualidade fisiológica das sementes e o uso da linha de solidificação do endosperma como indicativo da maturidade fisiológica das sementes. O plantio foi realizado em 30 de setembro de 1996. Amostras de sementes dos híbridos simples CD 1723 e CD 5501, bem como do híbrido duplo OC 705, foram colhidas em intervalos de quatro dias, durante 59 dias, iniciando-se a colheita no vigésimo terceiro dia após o florescimento feminino. As características avaliadas nas sementes foram o acúmulo de matéria seca, o conteúdo de umidade, a germinação, o vigor (pelos testes de frio e de envelhecimento acelerado, a formação da camada preta e o desenvolvimento da linha de solidificação do endosperma. A colheita dos tr

  15. [Dynamics of seed rain of Tripterygium hypoglaucum and soil seed bank].

    Science.gov (United States)

    Zhang, Zhi-Wei; Wei, Yong-Sheng; Liu, Xiang; Su, Shu; Qu, Xian-You; Wang, Chang-Hua

    2017-11-01

    Tripterygium hypoglaucum is an endangered species in arid areas of Xiannvshan Chongqing, China. The dynamic characteristics of seed rain and soil seed bank of T. hypoglaucum were studied in this paper.Results showed that T. hypoglaucum years of mature seeds distribution number up to October; the seed rain occurred from the last ten-day of September to in the first ten-day of November and the peak of scattered seed rain concentrated in the October.The numbers of soil seed bank at 2-5 cm soil layer,mainly concentrated in the 1.5-3.5 m range. T. hypoglaucum seeds to the wind as a force for transmission, the transmission ability is strong, but in the process of natural reproduction, full mature seed rate is low, the soil seed bank seeds seed short-lived factors these were unfavorable for the natural reproduction of T. hypoglaucum population. Copyright© by the Chinese Pharmaceutical Association.

  16. Factors influencing upon the incidence of seed migration in I-125 seed transperineal prostate implantation

    International Nuclear Information System (INIS)

    Itami, Jun; Onishi, Kayoko; Kanemura, Mikio

    2005-01-01

    Transperineal I-125 seed brachytherapy for prostate cancer is rapidly expanding in Japan. Seed migrations to lung and abdomen are well known complication in the seed brachytherapy. The rate of incidence and the predisposing factors were studied. From April 2004 through January 2005, 36 patients underwent transperineal I-125 seed brachytherapy for prostate cancer. In all patients loose I-125 seeds were inserted with Mick applicator according to modified peripheral loading pattern. One day, 1 week, and 1 month after the procedure, posteroanterior and lateral chest X-rays and abdominal X-ray were performed. Abdominal and chest seed migrations were seen in 11 (30.6%) and 14 (38.9%) patients, respectively. In total, 20 patients (55.6%) showed seed migrations. Forty-two I-125 seeds migrated out of 2,508 implanted seeds. Most of the migrations were seen until 1 month after the procedure. The preplanned number of the extraprostatic seeds had a statistically significant influence upon the incidence of seed migration. Seed migration is not a rare phenomenon in transperineal I-125 seed brachytherapy for prostate cancer. To confirm seed migration, X-ray examinations 1 month after the procedure are suited. At the preplanning, the number of extraprostatic seeds should be limited to minimal to decrease the incidence of seed migration. In future, the introduction of linked I-125 seeds is preferred. (author)

  17. Ash (Fraxinus excelsior) seed quality in relation to seed ...

    African Journals Online (AJOL)

    Jane

    2011-07-18

    Jul 18, 2011 ... bottles were seal-locked and kept in the accelerated ageing cham- ber at three ... Hundred (100) seeds of each treatment were performed in plastic ..... by heat stress: the activation state of Rubisco as a limiting factor in.

  18. Effects of seed fermentation method on seed germination and vigor ...

    African Journals Online (AJOL)

    BERTIN

    2013-11-27

    Nov 27, 2013 ... high, suggesting that wet soil is necessary for an on farm reliable evaluation of seed fermentation method in the .... with tap water, and sundried in ambient air until attaining 6 to 7% moisture. ..... arabica in Brazil. Int. J. Food ...

  19. Nutrient composition of Dacryodes edulis seed and seed coat mixture

    Directory of Open Access Journals (Sweden)

    C.U. OGUNKA-NNOKA

    2017-07-01

    Full Text Available This study investigated the nutrient composition of D. edulis seed and seed coat mixture. Qualitative and quantitative phytochemicals, proximate, and vitamin compositions were evaluated using standard methods. Saponins were very high, alkaloids, flavonoids, and tannins were high, while terpenoids were low, and glycosides, aldehydes, and steroids were absent. The quantitative phytochemical determination followed the order; saponin > kaempferol > rutin > catechin > tannin > sapogenin > lunamarine > phenol > ribalinidine > anthocyanin > oxalate > phytate. For the proximate composition, carbohydrates had the highest concentration, followed by lipids and fibre, while, protein concentration was the lowest. Vitamin E (5.42 mg/100g, vitamin C (3.24 mg/100g, and vitamin A (2.84 mg/100g were the highest occurring constituent vitamins while vitamin B12 (0.035 mg/100g and vitamin B2 (0.075 mg/100g were the least occurring vitamins. This study has shown the rich phytochemical composition of D. edulis seed and seed coat mixture while showing deficiencies in proteins, distinct vitamins, and ash contents.

  20. The role of seed priming in improving seed germination and ...

    African Journals Online (AJOL)

    win7

    2013-11-13

    Nov 13, 2013 ... As a result, fresh and dry weights of shoot and root were improved. Seed priming alleviated the inhibitory effect of salt stress on germination and seedling growth of maize under salt stress. ... Salinization is more spreading in irrigated lands because of inappropriate management of irrigation and drainage.

  1. Seed producer cooperatives in the Ethiopian seed sector and their role in seed supply improvement: A review

    NARCIS (Netherlands)

    Sisay, D.T.; Verhees, F.J.H.M.; Trijp, van J.C.M.

    2017-01-01

    The role of seed producer cooperatives (SPCs) in the Ethiopian seed sector and their contribution to seed supply improvement have received attention from researchers, policymakers, and development partners. However, limited work has been done in reviewing and documenting their involvement in the

  2. Forest Seed Collection, Processing,and Testing

    DEFF Research Database (Denmark)

    Schmidt, Lars Holger

    2016-01-01

    This chapter pertains to the techniques of capturing the best genetic quality seeds a seed source can produce at the optimal time of high physiological maturity and maintaining these qualities throughout the handling processes, all at a minimum cost. Different collection and processing techniques...... apply to different species, seed types, situations, and purposes. Yet the collection and processing toolbox contains a number of “standard” methods for most of these groups. Records and documentation help in evaluating “best practice” for future method improvement, and it helps in linking offspring...... to seed source. Conditions are set for short- and long-term seed storage by their inert storability physiology. The potential storage life of seed may for some robust “orthodox” species be several decades, while no available storage conditions can maintain viability for sensitive “recalcitrant” seed. Seed...

  3. Chemical Composition of Kapok (Ceibapentandra) Seed and ...

    African Journals Online (AJOL)

    -5 years. ... with a sharp steel knife from which healthy seeds were selected. ... Total carbohydrate was determined by difference. .... resistance to microbial attack of plant tissue (Taiz and. Zeiger, 1991 ... Maintenance of soybean seed quality in ...

  4. Inheritance of egusi seed type in watermelon.

    Science.gov (United States)

    Gusmini, G; Wehner, T C; Jarret, R L

    2004-01-01

    An unusual seed mutant in watermelon (Citrullus lanatus var. lanatus) has seeds with a fleshy pericarp, commonly called egusi seeds. The origin of the phenotype is unknown, but it is widely cultivated in Nigeria for the high protein and carbohydrate content of the edible seeds. Egusi seeds have a thick, fleshy pericarp that appears during the second to third week of fruit development. We studied the inheritance of this phenotype in crosses of normal seeded Charleston Gray and Calhoun Gray with two plant introduction accessions, PI 490383w and PI 560006, having the egusi seed type. We found that the egusi seed type is controlled by a single recessive gene, and the symbol eg was assigned. Copyright 2004 The American Genetic Association

  5. Seed technology training in the year 2000

    Directory of Open Access Journals (Sweden)

    McDonald M.B.

    1998-01-01

    Full Text Available Seed quality will remain the centerpiece of successful agricultural programs in the year 2000. As new changes occur in agriculture driven by advancements in biotechnology, seed enhancement technologies, a more diverse seed user clientele, and communication technologies, successful seed companies will require a knowledgeable and informed workforce to assure high seed quality. A new approach to seed technology training is professed that relies on the establishment of a three-institution consortium to achieve this objective. Advantages of the consortium are identified that emphasize the unique strengths of each institution, their geographic advantages representing major climactic/agricultural zones in the world, and differing approaches to seed technology training that are facilitated by increasing ease of global communication. This may be a better way to conduct seed technology training in the year 2000.

  6. Analysis of Seed Potato Systems in Ethiopia

    NARCIS (Netherlands)

    Hirpa, A.; Meuwissen, M.P.M.; Tesfaye, A.; Lommen, W.J.M.; Oude Lansink, A.G.J.M.; Tsegaye, A.; Struik, P.C.

    2010-01-01

    This study aimed to analyze the seed potato systems in Ethiopia, identify constraints and prioritize improvement options, combining desk research, rapid appraisal and formal surveys, expert elicitation, field observations and local knowledge. In Ethiopia, informal, alternative and formal seed

  7. Seed improvement by selection and invigoration Seleção e envigoramento como técnicas para o aprimoramento do desempenho de sementes

    Directory of Open Access Journals (Sweden)

    A.A. Powell

    1998-01-01

    Full Text Available The basis of differences in seed quality are described for two groups of crops, grain legumes and vegetables and approaches to the improvement of quality are proposed. In grain legumes the major factors affecting seed quality are imbibition damage, seed ageing and their interaction. It is proposed that use of seed vigour tests, specifically the electrical conductivity test, to identify the incidence of imbibition damage and ageing allows the selection of seed lots for sale and for use under different field conditions. Alternatively in grain legume species in which there is a genotypic influence on predisposition to imbibition damage the approach to seed improvement may lie through selection in breeding programmes. Thus identification of testa characteristics that favour slow imbibition would enable selection for these characteristics. Seed ageing is also the major cause of reduced seed quality in vegetable species, leading to slow and asynchronous germination. An approach to their seed improvement has been the development of seed invigoration treatments based on seed hydration. The principle of these treatments is described and several treatments outlined, with emphasis being given to aerated hydration, a treatment completed within 36h. The physiological basis of improvement by invigoration is discussed.Neste trabalho, são descritas diferenças básicas na qualidade de sementes de leguminosas e hortaliças, e propostos procedimentos para a melhoria de seu desempenho. Os principais fatores que afetam a qualidade de sementes de leguminosas são as injurias durante a embebição, o envelhecimento e a interação entre esses fatores. Considera-se que a utilização de testes de vigor, principalmente o de condutividade elétrica, para a identificação de injurias durante a embebição e o grau de envelhecimento, permite a seleção de lotes para a comercialização e uso sob diferentes condições de campo. A alternativa, em leguminosas, onde h

  8. Effects of seed and seedling predation by small mammals on ...

    African Journals Online (AJOL)

    Seed predation reduced seedling recruitment from seeds planted in March 1986 in mature fynbos, but ... Seed predation did not significantly reduce seedling recruitment from seed planted in July, August and ... AJOL African Journals Online.

  9. Use of linear discriminant function analysis in seed morphotype ...

    African Journals Online (AJOL)

    Use of linear discriminant function analysis in seed morphotype relationship study in 31 ... Data were collected on 100-seed weight, seed length and seed width. ... to the Mesoamerican gene pool, comprising the cultigroups Sieva-Big Lima, ...

  10. Challenges and opportunities for quality seed potato availability and ...

    African Journals Online (AJOL)

    ACSS

    Key words: Quality seed potato, seed system and challenges ... systems, inadequate seed production and ... informal sources of seed potato, despite ..... Directorate General for International. Co-operation (DGIC), Brussels,. Belgium. 1540pp.

  11. Efficient chaining of seeds in ordered trees

    OpenAIRE

    Allali, Julien; Chauve, Cédric; Ferraro, Pascal; Gaillard, Anne-Laure

    2010-01-01

    International audience; We consider here the problem of chaining seeds in ordered trees. Seeds are mappings between two trees Q and T and a chain is a subset of non overlapping seeds that is consistent with respect to postfix order and ancestrality. This problem is a natural extension of a similar problem for sequences, and has applications in computational biology, such as mining a database of RNA secondary structures. For the chaining problem with a set of m constant size seeds, we describe...

  12. Composition of jojoba seeds and foliage

    Energy Technology Data Exchange (ETDEWEB)

    Verbiscar, A.J.; Banigan, T.F.

    1978-01-01

    The desert shrub jojoba (Simmondsia chinensis) may be browsed by cattle. The seeds have about 50% oil but the extracted meal is at present unsuitable for feeding. Simmondsin, the most prevalent toxin, is present in seed, 2.3%, and in husks, leaves and twigs. Seeds contained another toxin, Simmondsin 2'-ferulate. The contents of oil, protein, carbohydrate and amino acids in seed are tabulated. 13 references.

  13. Drug Plant Seed Viability Preservation by Cryoconservation

    Directory of Open Access Journals (Sweden)

    Alexandra Sh. Dodonova

    2013-01-01

    Full Text Available The article considers the possibility of cryopreservation of seeds of several species of drug plants in Central Kazakhstan – Tanacetum ulutavicum, Niedzwedzkia semiretschenskia, Rhaponticum carthamoides. To increase the amount of viable seeds after liquid nitrogen freezing, we used different defrosting temperatures, deposited seeds with different moisture contents and used different containers for cryopreservation. Recommendations, concerning conditions of cryopreservation of seeds of these drug plant species were developed, basing on the obtained results.

  14. Training and research in seed technology. No quality seeds without skilled staff

    NARCIS (Netherlands)

    Groot, S.P.C.; Hilhorst, H.W.M.

    2016-01-01

    The seed industry in the Netherlands is the largest exporter of horticultural seeds, reaching farmers in every country of the world. High seed quality is one of the key factors of this success. Maintaining and increasing the level of seed qualtiy requires skilled staff, innovation and collaboration

  15. How seed orchard culture affects seed quality: experience with the southern pines

    Science.gov (United States)

    James P. Barnett

    1996-01-01

    Tree improvement programs have influenced significantly the quality of southern pine seeds produced when compared to collections from native stands. Seed orchard management practices such as fertilization can increase seed size and reduce seed dormancy. These result in the need for less complex pregermination treatments. Repeated cone collections from the same clones...

  16. Consistent individual differences in seed disperser quality in a seed-eating fish

    NARCIS (Netherlands)

    Pollux, Bart J.A.

    2017-01-01

    Animal-mediated seed dispersal (zoochory) is considered to be an important mechanism regulating biological processes at larger spatial scales. To date, intra-specific variation in seed disperser quality within seed-dispersing animals has not been studied. Here, I employed seed feeding trials to

  17. Securing Access to Seed: Social Relations and Sorghum Seed Exchange in Eastern Ethiopia

    NARCIS (Netherlands)

    McGuire, S.

    2008-01-01

    Access to seed is crucial for farming, though few studies investigate household-level access in the informal `farmer seed systems¿ which still supply most seed in poor countries. This paper uses empirical data of seed exchange practices for sorghum in eastern Ethiopia to analyze how social

  18. seed germination and seedlings growth

    African Journals Online (AJOL)

    STORAGESEVER

    2007-12-17

    Dec 17, 2007 ... however, abscisic acid (ABA) can counter this effect by keeping seeds ... Tris-HCl buffer at pH 8.8, with constant current set at 20 mA/gel. The gels were ..... on plants is rarely studied and the importance of 20E in the life cycle ...

  19. The Seed Is the Law

    Science.gov (United States)

    Antone, Eileen M.

    2005-01-01

    Since humanities arise from a specific place and from the people of that place, this article will focus on Peacemaker's revolutionary teachings about the seed of law. Long before the people from across the ocean arrived here on Turtle Island (North America) there was much warfare happening. According to John Mohawk (2001, para. 1), an Iroquoian…

  20. Seed production for fuel oils

    International Nuclear Information System (INIS)

    Mosca, G.

    1992-01-01

    With the aim of assessing commercialization prospects for vegetable oils to be used as diesel fuel alternatives, this paper provides maps indicating regional production quantities for soybean, rape and sunflower seeds in Italy. It then tables and discusses the results of energy input-output analyses carried out for rape and soybean oil production

  1. Hormones and tomato seed germination

    NARCIS (Netherlands)

    Liu, Y.

    1996-01-01

    Using GA- and ABA-deficient mutants, exogenous gibberellins (GAs), abscisic acid (ABA) and osmoticum, we studied the roles of GAs and ABA in the induction of cell cycle activities, internal free space formation and changes in water relations during seed development and imbibition in tomato. First of

  2. Spiny hopsage fruit and seed morphology

    Science.gov (United States)

    Nancy L. Shaw; Emerenciana G. Hurd; Marshall R. Haferkamp

    1996-01-01

    Rangeland seedings of spiny hopsage (Gruyia spinosa [Hook.] Moq.) may be made with either bracted utricles or seeds. Problems have resulted from inconsistent use of terminology describing these 2 structures and the fact their germination and seedling emergence is not the same with similar environmental conditions and seeding techniques. We examined...

  3. Galactinol as marker for seed longevity

    NARCIS (Netherlands)

    Souza Vidigal, De D.; Willems, L.A.J.; Arkel, van J.; Dekkers, S.J.W.; Hilhorst, H.W.M.; Bentsink, L.

    2016-01-01

    Reduced seed longevity or storability is a major problem in seed storage and contributes to increasedcosts in crop production. Here we investigated whether seed galactinol contents could be predictive forseed storability behavior in Arabidopsis, cabbage and tomato. The analyses revealed a positive

  4. Seed dormancy and germination : light and nitrate

    NARCIS (Netherlands)

    Hilhorst, H.W.M.

    1990-01-01

    One of the most important aspects of the life cycle of seed plants is the formation and development of seeds on the motherplant and the subsequent dispersal. An equally important element of the survival strategy is the ability of seeds to prevent germination in unfavorable

  5. Dormancy cycling in seeds: mechanisms and regulation

    NARCIS (Netherlands)

    Claessens, S.M.C.

    2012-01-01

    The life cycle of most plants starts, and ends, at the seed stage. In most species mature seeds are shed and dispersed on the ground. At this stage of its life cycle the seed may be dormant and will, by definition, not germinate under favourable conditions (Bewley, 1997).

    Seasonal

  6. Seed coat darkening in Cowpea bean

    Science.gov (United States)

    Seed coat of cowpea bean (Vigna unguiculata L. Walp) slowly browns to a darker color during storage. High temperature and humidity during storage might contribute to this color change. Variation in browning rate among seeds in a lot leads to a mixture of seed colors creating an unacceptable product...

  7. 7 CFR 947.12 - Seed potatoes.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Seed potatoes. 947.12 Section 947.12 Agriculture... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE IRISH POTATOES GROWN IN MODOC AND... Definitions § 947.12 Seed potatoes. Seed potatoes means and includes all potatoes officially certified and...

  8. 7 CFR 946.12 - Seed potatoes.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Seed potatoes. 946.12 Section 946.12 Agriculture... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE IRISH POTATOES GROWN IN WASHINGTON Order Regulating Handling Definitions § 946.12 Seed potatoes. Seed potatoes means and includes all...

  9. Running title: Water distribution in chickpea seeds

    African Journals Online (AJOL)

    agriphy20

    2012-07-24

    Jul 24, 2012 ... molecular mobility of cellular water in magnetically exposed seeds as compared to unexposed seeds. Analysis of ... protrusion takes place through the seed coat and absorption .... directly related to water activity (aw) of the cell water. (Gambhir ..... plants, including photosynthesis, respiration and enzymatic ...

  10. Strategies for Seed Propagation of Native Forbs

    Science.gov (United States)

    Susan E. Meyer

    2006-01-01

    Native forbs are an increasingly important component of container production for many public and private nurseries. Propagators are often called upon to grow species with unknown requirements. A systematic approach is required to obtain plants from seeds of these species, beginning with determining what is a propagule and evaluating seed quality. Next, seed dormancy...

  11. Inheritance of fresh seed dormancy in groundnut

    African Journals Online (AJOL)

    SERVER

    2008-02-19

    Feb 19, 2008 ... Seed dormancy has been defined as the failure of an intact, viable seed to ... expected ratio of 3 dormant : 1 non-dormant seeds in F2 generations of crosses of ICGV 87378 and ICGV ... ICGV 87378 x ICGV 86158. 163. 0. 163.

  12. Spinach seed quality - potential for combining seed size grading and chlorophyll flourescence sorting

    DEFF Research Database (Denmark)

    Deleuran, Lise Christina; Olesen, Merete Halkjær; Boelt, Birte

    2013-01-01

    might therefore improve the establishment of spinach for producers. Spinach seeds were harvested at five different times (H1, H2, H3, H4 and H5) starting 3 weeks before estimated optimum harvest time. The harvested seeds were sorted according to chlorophyll fluorescence (CF) and seed size. Two harvest.......5–3.25 mm size seeds had germinated on day 3 than both their larger and smaller counterparts at the later time of harvest (H4). Seeds with a diameter below 2.5 mm displayed the lowest MGT. Commercially, the use of chlorophyll fluorescence (CF)-sorted seeds, in combination with seed size sorting, may provide...

  13. [Effects of illumination and seed-soaking reagent on seed germination of Solanum nigrum].

    Science.gov (United States)

    Yang, Chuan-Jie; Wei, Shu-He; Zhou, Qi-Xing; Hu, Ya-Hu; Niu, Rong-Cheng

    2009-05-01

    To explore a rapid seed germination method for hyperaccumulator Solanum nigrum, a germination experiment with different illumination and seed-soaking treatments was conducted in constant temperature box and greenhouse, with filter as burgeon base. Under illumination, the germination rate was about 5 times high of that without illumination (P seed germination of S. nigrum. All test seed-soaking reagents could significantly improve the germination rate of S. nigrum (P seeds treated with H2O2 had the shortest germination time. The germination rate of seeds soaked but without cleaning was 2-3 times as high as that of seeds soaked and cleaned with water.

  14. The biomechanics of seed germination.

    Science.gov (United States)

    Steinbrecher, Tina; Leubner-Metzger, Gerhard

    2017-02-01

    From a biomechanical perspective, the completion of seed (and fruit) germination depends on the balance of two opposing forces: the growth potential of the embryonic axis (radicle-hypocotyl growth zone) and the restraint of the seed-covering layers (endosperm, testa, and pericarp). The diverse seed tissues are composite materials which differ in their dynamic properties based on their distinct cell wall composition and water uptake capacities. The biomechanics of embryo cell growth during seed germination depend on irreversible cell wall loosening followed by water uptake due to the decreasing turgor, and this leads to embryo elongation and eventually radicle emergence. Endosperm weakening as a prerequisite for radicle emergence is a widespread phenomenon among angiosperms. Research into the biochemistry and biomechanics of endosperm weakening has demonstrated that the reduction in puncture force of a seed's micropylar endosperm is environmentally and hormonally regulated and involves tissue-specific expression of cell wall remodelling proteins such as expansins, diverse hydrolases, and the production of directly acting apoplastic reactive oxygen. The endosperm-weakening biomechanics and its underlying cell wall biochemistry differ between the micropylar (ME) and chalazal (CE) endosperm domains. In the ME, they involve cell wall loosening, cell separation, and programmed cell death to provide decreased and localized ME tissue resistance, autolysis, and finally the formation of an ME hole required for radicle emergence. Future work will further unravel the molecular mechanisms, environmental regulation, and evolution of the diverse biomechanical cell wall changes underpinning the control of germination by endosperm weakening. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  15. Improving seed potato quality in Ethiopia: a value chain perspective

    NARCIS (Netherlands)

    Hirpa, A.; Gielen-Meuwissen, M.P.M.; Lommen, W.J.M.; Oude Lansink, A.G.J.M.; Tsegaye, A.; Struik, Paul

    2016-01-01

    In Ethiopia, use of low-quality seed potatoes by the majority of potato growers is
    associated with underdevelopment of the seed potato value chains. Three seed potato systems are present in Ethiopia: the informal seed system, the alternative seed system and the formal seed system. This chapter

  16. Evaluation of seed production of scots pine ( Pinus sylvestris L ...

    African Journals Online (AJOL)

    This research was carried out to investigate seed production in a 13 years-old scots pine (Pinus sylvestris L.) clonal seed orchard, including 30 clones. Eight of cone and seed traits as number of fertile and infertile scales, cone volume, cone number, filled and empty seed number, seed efficiency and 1000 seed weight were ...

  17. WE-A-17A-11: Implanted Brachytherapy Seed Movement Due to Transrectal Ultrasound Probe-Induced Prostate Deformation

    Energy Technology Data Exchange (ETDEWEB)

    Liu, D; Usmani, N; Sloboda, R [Cross Cancer Institute, Edmonton, Alberta (Canada); University of Alberta, Edmonton, Alberta (Canada); Meyer, T; Husain, S; Angyalfi, S [Tom Baker Cancer Centre, Calgary, Alberta (Canada); University of Calgary, Calgary, Alberta (Canada); Kay, I [Canterbury District Health Board, Christchurch (New Zealand)

    2014-06-15

    Purpose: To characterize the movement of implanted brachytherapy seeds due to transrectal ultrasound probe-induced prostate deformation and to estimate the effects on prostate dosimetry. Methods: Implanted probe-in and probe-removed seed distributions were reconstructed for 10 patients using C-arm fluoroscopy imaging. The prostate was delineated on ultrasound and registered to the fluoroscopy seeds using a visible subset of seeds and residual needle tracks. A linear tensor and shearing model correlated the seed movement with position. The seed movement model was used to infer the underlying prostate deformation and to simulate the prostate contour without probe compression. Changes in prostate and surrogate urethra dosimetry were calculated. Results: Seed movement patterns reflecting elastic decompression, lateral shearing, and rectal bending were observed. Elastic decompression was characterized by anterior-posterior expansion and superior-inferior and lateral contractions. For lateral shearing, anterior movement up to 6 mm was observed for extraprostatic seeds in the lateral peripheral region. The average intra-prostatic seed movement was 1.3 mm, and the residual after linear modeling was 0.6 mm. Prostate D90 increased by 4 Gy on average (8 Gy max) and was correlated with elastic decompression. For selected patients, lateral shearing resulted in differential change in D90 of 7 Gy between anterior and posterior quadrants, and increase in whole prostate D90 of 4 Gy. Urethra D10 increased by 4 Gy. Conclusion: Seed movement upon probe removal was characterized. The proposed model captured the linear correlation between seed movement and position. Whole prostate dose coverage increased slightly, due to the small but systematic seed movement associated with elastic decompression. Lateral shearing movement increased dose coverage in the anterior-lateral region, at the expense of the posterior-lateral region. The effect on whole prostate D90 was smaller due to the subset

  18. Multivariate NIR studies of seed-water interaction in Scots Pine Seeds (Pinus sylvestris L.)

    OpenAIRE

    Lestander, Torbjörn

    2003-01-01

    This thesis describes seed-water interaction using near infrared (NIR) spectroscopy, multivariate regression models and Scots pine seeds. The presented research covers classification of seed viability, prediction of seed moisture content, selection of NIR wavelengths and interpretation of seed-water interaction modelled and analysed by principal component analysis, ordinary least squares (OLS), partial least squares (PLS), bi-orthogonal least squares (BPLS) and genetic algorithms. The potenti...

  19. Influence the Rubber Seed Type and Altitude on Characteristic of Seed, Oil and Biodiesel

    OpenAIRE

    Salni Salni; Poedji Loekitowati Hariani; Hanifa Marisa Hanifa

    2017-01-01

    This research studies the influence of the type of rubber seed that is superior and local, altitude plant in South Sumatra province to the characteristic of seed, oil and biodiesel (methyl ester). Rubber plants planted from local rubber seed by seeds seedlings and superior rubber seed by selected clones. In the study, rubber plants planted at a different altitude, namely in Banyuasin district (18 m above sea level), Prabumulih District (176 m above sea level) and Lahat District (627 m above s...

  20. Influence of the Rubber Seed Type and Altitude on Characteristic of Seed, Oil and Biodiesel

    OpenAIRE

    Salni, Salni; Hariani, Poedji Loekitowati; Hanifa, Hanifa Marisa

    2017-01-01

    This research studies the influence of the type of rubber seed that is superior and local, altitude plant in South Sumatra province to the characteristic of seed, oil and biodiesel (methyl ester). Rubber plants planted from local rubber seed by seeds seedlings and superior rubber seed by selected clones. In the study, rubber plants planted at a different altitude, namely in Banyuasin district (18 m above sea level), Prabumulih District (176 m above sea level) and Lahat District (627 m above s...

  1. Operator-free, film-based 3D seed reconstruction in brachytherapy

    International Nuclear Information System (INIS)

    Todor, D.A.; Cohen, G.N.; Amols, H.I.; Zaider, M.

    2002-01-01

    In brachytherapy implants, the accuracy of dose calculation depends on the ability to localize radioactive sources correctly. If performed manually using planar images, this is a time-consuming and often error-prone process - primarily because each seed must be identified on (at least) two films. In principle, three films should allow automatic seed identification and position reconstruction; however, practical implementation of the numerous algorithms proposed so far appears to have only limited reliability. The motivation behind this work is to create a fast and reliable system for real-time implant evaluation using digital planar images obtained from radiotherapy simulators, or mobile x-ray/fluoroscopy systems. We have developed algorithms and code for 3D seed coordinate reconstruction. The input consists of projections of seed positions in each of three isocentric images taken at arbitrary angles. The method proposed here consists of a set of heuristic rules (in a sense, a learning algorithm) that attempts to minimize seed misclassifications. In the clinic, this means that the system must be impervious to errors resulting from patient motion as well as from finite tolerances accepted in equipment settings. The software program was tested with simulated data, a pelvic phantom and patient data. One hundred and twenty permanent prostate implants were examined (105 125 I and 15 103 Pd) with the number of seeds ranging from 35 to 138 (average 79). The mean distance between actual and reconstructed seed positions is in the range 0.03-0.11 cm. On a Pentium III computer at 600 MHz the reconstruction process takes 10-30 s. The total number of seeds is independently validated. The process is robust and able to account for errors introduced in the clinic. (author)

  2. Operator-free, film-based 3D seed reconstruction in brachytherapy

    Energy Technology Data Exchange (ETDEWEB)

    Todor, D.A.; Cohen, G.N.; Amols, H.I.; Zaider, M. [Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, NY (United States)

    2002-06-21

    In brachytherapy implants, the accuracy of dose calculation depends on the ability to localize radioactive sources correctly. If performed manually using planar images, this is a time-consuming and often error-prone process - primarily because each seed must be identified on (at least) two films. In principle, three films should allow automatic seed identification and position reconstruction; however, practical implementation of the numerous algorithms proposed so far appears to have only limited reliability. The motivation behind this work is to create a fast and reliable system for real-time implant evaluation using digital planar images obtained from radiotherapy simulators, or mobile x-ray/fluoroscopy systems. We have developed algorithms and code for 3D seed coordinate reconstruction. The input consists of projections of seed positions in each of three isocentric images taken at arbitrary angles. The method proposed here consists of a set of heuristic rules (in a sense, a learning algorithm) that attempts to minimize seed misclassifications. In the clinic, this means that the system must be impervious to errors resulting from patient motion as well as from finite tolerances accepted in equipment settings. The software program was tested with simulated data, a pelvic phantom and patient data. One hundred and twenty permanent prostate implants were examined (105{sup 125}I and 15{sup 103}Pd) with the number of seeds ranging from 35 to 138 (average 79). The mean distance between actual and reconstructed seed positions is in the range 0.03-0.11 cm. On a Pentium III computer at 600 MHz the reconstruction process takes 10-30 s. The total number of seeds is independently validated. The process is robust and able to account for errors introduced in the clinic. (author)

  3. Seed yield and physiological quality of soybean in different environmentsProdução e qualidade fisiológica de sementes de soja em diferentes ambientes de cultivo

    Directory of Open Access Journals (Sweden)

    Cristiano Lemes da Silva

    2012-12-01

    Full Text Available The objective of this study was to evaluate the physiological quality and seed yield of soybean cultivars produced in Campo Mourão and Guarapuava, Paraná State, and Guaíra in the São Paulo State. Seeds of nine cultivars from trials network of the Fundação Pró-Sementes were used for analysis. The experimental design was in randomized blocks in factorial scheme 3 x 9 (cultivars x environments, with three replications. The evaluation of seed quality was performed using the germination and vigor tests, and through the accelerated aging and Tetrazolium tests. The results showed that the physiological quality and seed yield were influenced by the sowing environment. Soybeans seeds from the region of Guarapuava-PR had higher germination, vigour and viability, compared the regions of Campo Mourão-PR and Guaíra-SP. The mechanical and moisture damage were mainly causes for the decrease in germination, vigor and viability of seeds at all regions evaluated. The cultivars CD 214 RR, BRS 246 and BRS 255 RR showed high yield and seed quality. O objetivo deste estudo foi avaliar a qualidade fisiológica e o rendimento de sementes de diferentes cultivares de soja produzida nos municípios de Campo Mourão e Guarapuava, Estado do Paraná e, Guaíra no Estado de São Paulo. Sementes de nove cultivares, provenientes da rede de ensaios da Fundação Pró-Sementes, foram utilizadas para as análises. O delineamento experimental foi em blocos ao acaso, em esquema fatorial 9 x 3 (cultivares x ambientes, com 3 repetições. A avaliação da qualidade da semente foi realizada pelo teste de germinação e vigor, e pelos testes de Envelhecimento Precoce e Tetrazólio. Verificou-se que a qualidade fisiológica e o rendimento das sementes foi influenciada pelo ambiente de cultivo. Sementes de soja provenientes da região de Guarapuava-PR apresentam maior germinação, vigor e viabilidade, em comparação as regiões de Campo Mourão-PR e Guaíra-SP. Os danos mec

  4. [Procedure of seed quality testing and seed grading standard of Prunus humilis].

    Science.gov (United States)

    Wen, Hao; Ren, Guang-Xi; Gao, Ya; Luo, Jun; Liu, Chun-Sheng; Li, Wei-Dong

    2014-11-01

    So far there exists no corresponding quality test procedures and grading standards for the seed of Prunus humilis, which is one of the important source of base of semen pruni. Therefor we set up test procedures that are adapt to characteristics of the P. humilis seed through the study of the test of sampling, seed purity, thousand-grain weight, seed moisture, seed viability and germination percentage. 50 cases of seed specimens of P. humilis tested. The related data were analyzed by cluster analysis. Through this research, the seed quality test procedure was developed, and the seed quality grading standard was formulated. The seed quality of each grade should meet the following requirements: for first grade seeds, germination percentage ≥ 68%, thousand-grain weight 383 g, purity ≥ 93%, seed moisture ≤ 5%; for second grade seeds, germination percentage ≥ 26%, thousand-grain weight ≥ 266 g, purity ≥ 73%, seed moisture ≤9%; for third grade seeds, germination percentage ≥ 10%, purity ≥ 50%, thousand-grain weight ≥ 08 g, seed moisture ≤ 13%.

  5. Myrmecochory and short-term seed fate in Rhamnus alaternus: Ant species and seed characteristics

    Science.gov (United States)

    Bas, J. M.; Oliveras, J.; Gómez, C.

    2009-05-01

    Benefits conferred on plants in ant-mediated seed dispersal mutualisms (myrmecochory) depend on the fate of transported seeds. We studied the effects of elaiosome presence, seed size and seed treatment (with and without passage through a bird's digestive tract) on short-term seed fate in Rhamnus alaternus. In our study, we define short-term seed, or initial, seed fate, as the location where ants release the seeds after ant contact with it. The elaiosomes had the most influence on short-term fate, i.e. whether or not seeds were transported to the nest. The workers usually transported big seeds more often than small ones, but small ants did not transport large seeds. Effect of seed size on transport depended on the ant species and on the treatment of the seed (manual extraction simulating a direct fall from the parent plant vs. bird deposition corresponding to preliminary primary dispersal). Probability of removal of elaiosome-bearing seeds to the nest by Aphaenogaster senilis increased with increasing seed weight.

  6. Trade and Transfer of Tree Seed

    DEFF Research Database (Denmark)

    Schmidt, Lars Holger

    2016-01-01

    testing records. Genetic quality is documented as documents on origin or seed source. New types of tree planting by smallholders imply special problems in distribution and supply systems since production systems for tree seeds have large areas while many consumers have small space for planting....... A centralized forest seed supply contains large central units with good facilities for production and procurement but is far from seed users. Alternative decentralized systems with many small producers may have problems meeting high standards of seed quality and dealing with central regulations....

  7. Stimulation of lettuce seed germination by ethylene.

    Science.gov (United States)

    Abeles, F B; Lonski, J

    1969-02-01

    Ethylene increased the germination of freshly imbibed lettuce (Lactuca sativa L. var. Grand Rapids) seeds. Seeds receiving either red or far-red light or darkness all showed a positive response to the gas. However, ethylene was apparently without effect on dormant seeds, those which failed to germinate after an initial red or far-red treatment. Carbon dioxide, which often acts as a competitive inhibitor of ethylene, failed to clearly reverse ethylene-enhanced seed germination. While light doubled ethylene production from the lettuce seeds, its effect was not mediated by the phytochrome system since both red and far-red light had a similar effect.

  8. GERMINATION STUDIES ON Tabebuia impetiginosa Mart. SEEDS

    Directory of Open Access Journals (Sweden)

    Edvaldo Aparecido Amaral da Silva

    2004-06-01

    Full Text Available Seed germination and seedling production of native forest tree species are an important step in ex situ conservation programs and in the reforestation with ecological purposes. Therefore, understanding seed germination and its regulation is mandatory for the complete success of the conservation programs and revegetation techniques. Thus, morphological studies, temperature requirements for seed germination and its control by gibberellins (GAs were studied in Tabebuia impetiginosa (“ipê-roxo” seeds. The best temperature for germination under constant light was 30oC. The imbibition of T. impetiginosa seeds followed the common triphasic pattern, with most of the seeds attaining phase II at 24 hours and phase III at 72 hours of imbibition. Visible germination, as radicle elongation, started at 30 hours in water-imbibed seeds and at 24 hours in GA-imbibed seeds. Seeds imbibed in Paclobutrazol, an inhibitor of GA biosynthesis, failed to germinate. However, application of exogenous gibberellins overcame inhibition and allowed germination, suggesting that GAs are regulators of Tabebuia impetiginosa seed germination. The results suggested that germination in Tabebuia impetiginosa seeds is controlled by elongation of the radicle and gibberellins may play an important role in regulating it. The possible role of gibberellins is discussed.

  9. [Seed quality test methods of Paeonia suffruticosa].

    Science.gov (United States)

    Cao, Ya-Yue; Zhu, Zai-Biao; Guo, Qiao-Sheng; Liu, Li; Wang, Chang-Lin

    2014-11-01

    In order to optimize the testing methods for Paeonia suffruticosa seed quality, and provide basis for establishing seed testing rules and seed quality standard of P. suffruticosa. The seed quality of P. suffruticosa from different producing areas was measured based on the related seed testing regulations. The seed testing methods for quality items of P. suffruticosa was established preliminarily. The samples weight of P. suffruticosa was at least 7 000 g for purity analysis and was at least 700 g for test. The phenotypic observation and size measurement were used for authenticity testing. The 1 000-seed weight was determined by 100-seed method, and the water content was carried out by low temperature drying method (10 hours). After soaking in distilled water for 24 h, the seeds was treated with different temperature stratifications of day and night (25 degrees C/20 degrees C, day/night) in the dark for 60 d. After soaking in the liquor of GA3 300 mg x L(-1) for 24 h, the P. suffruticos seeds were cultured in wet sand at 15 degrees C for 12-60 days for germination testing. Seed viability was tested by TlC method.

  10. Is seed conditioning essential for Orobanche germination?

    Science.gov (United States)

    Plakhine, Dina; Ziadna, Hammam; Joel, Daniel M

    2009-05-01

    Parasitic Orobanchaceae germinate only after receiving a chemical stimulus from roots of potential host plants. A preparatory phase of several days that follows seed imbibition, termed conditioning, is known to be required; thereafter the seeds can respond to germination stimulants. The aim of this study was to examine whether conditioning is essential for stimulant receptivity. Non-conditioned seeds of both Orobanche cumana Wallr. and O. aegyptiaca Pers. [syn. Phelipanche aegyptiaca (Pers.) Pomel] were able to germinate in response to chemical stimulation by GR24 even without prior conditioning. Stimulated seeds reached maximal germination rates about 2 weeks after the onset of imbibition, no matter whether the seeds had or had not been conditioned before stimulation. Whereas the lag time between stimulation and germination response of non-conditioned seeds was longer than for conditioned seeds, the total time between imbibition and germination was shorter for the non-conditioned seeds. Unlike the above two species, O. crenata Forsk. was found to require conditioning prior to stimulation. Seeds of O. cumana and O. aegyptiaca are already receptive before conditioning. Thus, conditioning is not involved in stimulant receptivity. A hypothesis is put forward, suggesting that conditioning includes (a) a parasite-specific early phase that allows the imbibed seeds to overcome the stress caused by failing to receive an immediate germination stimulus, and (b) a non-specific later phase that is identical to the pregermination phase between seed imbibition and actual germination that is typical for all higher plants.

  11. Seed counting system evaluation using arduino microcontroller

    Directory of Open Access Journals (Sweden)

    Paulo Fernando Escobar Paim

    2018-01-01

    Full Text Available The development of automated systems has been highlighted in the most diverse productive sectors, among them, the agricultural sector. These systems aim to optimize activities by increasing operational efficiency and quality of work. In this sense, the present work has the objective of evaluating a prototype developed for seed count in laboratory, using Arduino microcontroller. The prototype of the system for seed counting was built using a dosing mechanism commonly used in seeders, electric motor, Arduino Uno, light dependent resistor and light emitting diode. To test the prototype, a completely randomized design (CRD was used in a two-factorial scheme composed of three groups defined according to the number of seeds (500, 1000 and 1500 seeds tested, three speeds of the dosing disc that allowed the distribution in 17, 21 and 32 seeds per second, with 40 repetitions evaluating the seed counting prototype performance in different speeds. The prototype of the bench counter showed a moderate variability of seed number of counted within the nine tests and a high precision in the seed count on the distribution speeds of 17 and 21 seeds per second (s-1 up to 1500 seeds tested. Therefore, based on the observed results, the developed prototype presents itself as an excellent tool for counting seeds in laboratory.

  12. Interactions between seed traits and digestive processes determine the germinability of bird-dispersed seeds.

    Science.gov (United States)

    Kleyheeg, Erik; Claessens, Mascha; Soons, Merel B

    2018-01-01

    Waterbirds disperse a wide range of plant seeds via their guts, promoting biotic connectivity between isolated habitat patches. However, the intensity of digestive forces encountered by seeds, and therefore their potential to survive digestive tract passage, varies within and between waterbird species. Here, we investigate under controlled conditions how the interaction between seed traits and digestive strategies affect the germinability of seeds following waterbird-mediated dispersal. We exposed seeds of 30 wetland plant species to the main digestive processes in the dabbling duck digestive system: mechanical, chemical and intestinal digestion. These were simulated by 1) a pressure test and scarification treatment, 2) incubation in simulated gastric juice, and 3) incubation in intestinal contents of culled mallards (Anas platyrhynchos). We evaluated their separate and combined effects on seed germination, and identified the role of seed size and seed coat traits in resisting the digestive forces. Seeds were generally resistant to separate digestive processes, but highly sensitive to a combination. Resistance to mechanical break-down was reduced by up to 80% by chemical pre-treatment, especially for seeds with permeable coats. Scarified seeds were 12-17% more vulnerable to chemical and intestinal digestive processes than undamaged seeds. Large seeds and seeds with thin, permeable coats were particularly sensitive to chemical and intestinal digestion. These results indicate that efficient digestion of seeds requires multiple digestive processes. The gizzard, responsible for mechanical digestion, plays a key role in seed survival. Omnivorous birds, which have relatively light gizzards compared to pure herbivores or granivores, are thus most likely to disperse seeds successfully. Regardless of digestive strategy, small seeds with tough seed coats are most resistant to digestion and may be adapted to endozoochorous dispersal by waterbirds.

  13. Interactions between seed traits and digestive processes determine the germinability of bird-dispersed seeds

    Science.gov (United States)

    Soons, Merel B.

    2018-01-01

    Waterbirds disperse a wide range of plant seeds via their guts, promoting biotic connectivity between isolated habitat patches. However, the intensity of digestive forces encountered by seeds, and therefore their potential to survive digestive tract passage, varies within and between waterbird species. Here, we investigate under controlled conditions how the interaction between seed traits and digestive strategies affect the germinability of seeds following waterbird-mediated dispersal. We exposed seeds of 30 wetland plant species to the main digestive processes in the dabbling duck digestive system: mechanical, chemical and intestinal digestion. These were simulated by 1) a pressure test and scarification treatment, 2) incubation in simulated gastric juice, and 3) incubation in intestinal contents of culled mallards (Anas platyrhynchos). We evaluated their separate and combined effects on seed germination, and identified the role of seed size and seed coat traits in resisting the digestive forces. Seeds were generally resistant to separate digestive processes, but highly sensitive to a combination. Resistance to mechanical break-down was reduced by up to 80% by chemical pre-treatment, especially for seeds with permeable coats. Scarified seeds were 12–17% more vulnerable to chemical and intestinal digestive processes than undamaged seeds. Large seeds and seeds with thin, permeable coats were particularly sensitive to chemical and intestinal digestion. These results indicate that efficient digestion of seeds requires multiple digestive processes. The gizzard, responsible for mechanical digestion, plays a key role in seed survival. Omnivorous birds, which have relatively light gizzards compared to pure herbivores or granivores, are thus most likely to disperse seeds successfully. Regardless of digestive strategy, small seeds with tough seed coats are most resistant to digestion and may be adapted to endozoochorous dispersal by waterbirds. PMID:29614085

  14. Evaluation of Wheat (Triticum aestivum, L. Seed Quality of Certified Seed and Farm-Saved Seed in Three Provinces of Iran

    Directory of Open Access Journals (Sweden)

    Khazaei Fardin

    2016-06-01

    Full Text Available The objective of this study was to study the seed quality aspects of wheat (Triticum aestivum L. and the extent of weed seed contamination present in wheat seeds produced in different regions of Iran. Four districts (cities, each including 12 fields (six certified seed fields and six farm-saved seed fields, were selected in each regions (provinces. One kilogram of the wheat seed sample was collected from each field for analysis in the laboratory. Wheat seeding was commonly done by farm-saved seed sourced from within the farm due to the high costs of certified seeds purchased from outside sources, followed by the low seed quality. The use of a farm-saved seed resulted in a higher germination rate and a lower mean time to germination compared with another system. The more positive temperatures experienced by mother plants could decrease the number of normal seedling and seedling length vigor index. Generally there was virtually no difference about physiological quality between certified seed and farm-saved seed sector that is related to lower quality of certified seed. The certified produced seeds had the lower number of weed seed, species and genus before and after cleaning. The highest seed purity and 1000 seed weight was obtained from the certified seed production system. The need for cleaning the farm-saved seed samples before sowing is one of the important findings of this survey.

  15. Testes de vigor em sementes de alface Seed vigor tests for lettuce seeds

    Directory of Open Access Journals (Sweden)

    Ana Lúcia P Kikuti

    2012-03-01

    Full Text Available O vigor da semente tem sido avaliado por meio de testes fisiológicos, bioquímicos e de tolerância a estresse. Mais recentemente, com o uso da análise computadorizada de imagens de plântulas, podem ser obtidas informações objetivas, em período relativamente curto, com menor interferência humana. O objetivo nesta pesquisa foi identificar testes eficientes para avaliação do vigor de sementes de alface, com ênfase para a análise de imagens digitais de plântulas. Sementes de 12 lotes (seis da cultivar Vanda e seis da Vera foram avaliadas por meio de testes de germinação, germinação a baixa temperatura (15ºC, emergência de plântulas e envelhecimento acelerado tradicional e com solução salina (NaCl 40% saturada, a 41ºC/48 h e 41ºC/72 h. Utilizou-se, também, o sistema de análise computadorizada de imagens de plântulas (SVIS®, desenvolvido pela Ohio State University (EUA, que forneceu dados referentes ao índice de vigor, uniformidade e crescimento de plântulas. Os testes foram realizados a cada três meses, durante 12 meses de armazenamento, sob condições ambiente. Com base nos resultados obtidos, concluiu-se que os testes de envelhecimento acelerado com uso de solução saturada de NaCl, a 41ºC por 48 e 72 horas e de velocidade de germinação, e os parâmetros obtidos com o software SVIS, índice de crescimento de plântulas e comprimento da raiz primária, são eficientes na avaliação do vigor de lotes de sementes de alface.The seed vigor is evaluated by traditional tests and more recently by seedling imaging analysis to obtain objective information within a short period of time. The objective of the study was to verify the accuracy of accepted vigor tests in comparison to an automated computer imaging system for seedling evaluation (SVIS® in order to assess lettuce seed vigor. Six seed lots each of two cultivars (Vanda and Vera were evaluated in the germination, cold germination (15ºC, seedling emergence and

  16. Primary Occipital Ewing’s Sarcoma with Subsequent Spinal Seeding

    Directory of Open Access Journals (Sweden)

    Ali Alqahtani

    2017-01-01

    Full Text Available Ewing’s sarcoma is a primary bone cancer that mainly affects the long bones. This malignancy is particularly common in pediatric patients. Primary cranial involvement accounts for 1% of cases, with occipital involvement considered extremely rare. In this case study, primary occipital Ewing’s sarcoma with a posterior fossa mass and subsequent relapse resulting in spinal seeding is reported. A 3-year-old patient presented with a 1-year history of left-sided headaches, localized over the occipital bone with progressive torticollis. Computed tomography (CT imaging showed a mass in the left posterior fossa compressing the brainstem. The patient then underwent surgical excision followed by adjuvant chemoradiation therapy. Two years later, the patient presented with severe lower back pain and urinary incontinence. Whole-spine magnetic resonance imaging (MRI showed cerebrospinal fluid (CSF seeding from the L5 to the S4 vertebrae. Primary cranial Ewing’s sarcoma is considered in the differential diagnosis of children with extra-axial posterior fossa mass associated with destructive permeative bone lesions. Although primary cranial Ewing’s sarcoma typically has good prognosis, our patient developed metastasis in the lower spine. Therefore, with CNS Ewing’s sarcoma, screening of the entire neural axis should be taken into consideration for early detection of CSF seeding metastasis in order to decrease the associated morbidity and mortality.

  17. Primary Occipital Ewing's Sarcoma with Subsequent Spinal Seeding.

    Science.gov (United States)

    Alqahtani, Ali; Amer, Roaa; Bakhsh, Eman

    2017-01-01

    Ewing's sarcoma is a primary bone cancer that mainly affects the long bones. This malignancy is particularly common in pediatric patients. Primary cranial involvement accounts for 1% of cases, with occipital involvement considered extremely rare. In this case study, primary occipital Ewing's sarcoma with a posterior fossa mass and subsequent relapse resulting in spinal seeding is reported. A 3-year-old patient presented with a 1-year history of left-sided headaches, localized over the occipital bone with progressive torticollis. Computed tomography (CT) imaging showed a mass in the left posterior fossa compressing the brainstem. The patient then underwent surgical excision followed by adjuvant chemoradiation therapy. Two years later, the patient presented with severe lower back pain and urinary incontinence. Whole-spine magnetic resonance imaging (MRI) showed cerebrospinal fluid (CSF) seeding from the L5 to the S4 vertebrae. Primary cranial Ewing's sarcoma is considered in the differential diagnosis of children with extra-axial posterior fossa mass associated with destructive permeative bone lesions. Although primary cranial Ewing's sarcoma typically has good prognosis, our patient developed metastasis in the lower spine. Therefore, with CNS Ewing's sarcoma, screening of the entire neural axis should be taken into consideration for early detection of CSF seeding metastasis in order to decrease the associated morbidity and mortality.

  18. Noahs Ark of the seeds

    International Nuclear Information System (INIS)

    Tristan, R. M.

    2015-01-01

    The so-called 'vault of the end of the world', located in the archipelago Arctic Svalbard, brings together more than 860,000 samples seeds from around the world. The purpose of your existence -like the of thousands of other banks of its kind in all the planet - is to preserve the genetic diversity of crops, most of them are now disappearing. An international treaty approved by the United Nations, and in force since 2004, allowed the construction of this 'Sanctuary' where more than one hundred countries maintain their own banks of seeds, freezing them to 18 degrees below zero to lower its biological activity. (Author)

  19. An Experimental Study on the aerodynamic and aeroacoustic performances of Maple-Seed-Inspired UAV Propellers

    Science.gov (United States)

    Hu, Hui; Ning, Zhe

    2016-11-01

    Due to the auto-rotating trait of maple seeds during falling down process, flow characteristics of rotating maple seeds have been studied by many researchers in recent years. In the present study, an experimental investigation was performed to explore maple-seed-inspired UAV propellers for improved aerodynamic and aeroacoustic performances. Inspired by the auto-rotating trait of maple seeds, the shape of a maple seed is leveraged for the planform design of UAV propellers. The aerodynamic and aeroacoustic performances of the maple-seed-inspired propellers are examined in great details, in comparison with a commercially available UAV propeller purchased on the market (i.e., a baseline propeller). During the experiments, in addition to measuring the aerodynamic forces generated by the maple-seed-inspired propellers and the baseline propeller, a high-resolution Particle Image Velocimetry (PIV) system was used to quantify the unsteady flow structures in the wakes of the propellers. The aeroacoustic characteristics of the propellers are also evaluated by leveraging an anechoic chamber available at the Aerospace Engineering Department of Iowa State University. The research work is supported by National Science Foundation under Award Numbers of OSIE-1064235.

  20. Nuclear microprobe studies of elemental distributions in dormant seeds of Burkea africana

    Science.gov (United States)

    Witkowski, E. T. F.; Weiersbye-Witkowski, I. M.; Przybyłowicz, W. J.; Mesjasz-Przybyłowicz, J.

    1997-07-01

    Seed nutrient stores are vital post-germination for the establishment of seedlings in harsh and unpredictable environments. Plants of nutrient-poor environments allocate a substantial proportion of total acquired nutrients to reproduction (i.e. seeds). We propose that differential allocation of mineral resources to specific seed tissues is an indication of a species germination and establishment strategy. Burkea africana Hook is a leguminous tree typical of broad-leaved nutrient-poor savannas in southern Africa. Elemental distributions in dormant B. africana seed structures were obtained using the true elemental imaging system (Dynamic Analysis) of the NAC Van de Graaff nuclear microprobe. Raster scans of 3.0 MeV protons were complemented by simultaneous BS and PIXE point analyses. Mineral nutrient concentrations varied greatly between seed tissues. Elevated levels of metals known to play an important role as plant enzyme co-factors were found in the seed lens and embryonic axis. Distributions of most of these metals (Ca, Mn, Fe and Zn, but not K or Cu) were positively correlated with embryonic P distribution, and probably represent phytin deposits. The distribution of metals within seed structures is 'patchy' due to their complexation with P as electron-dense globoid phytin crystals, which constrains the interpretation of PIXE point analyses.

  1. Adaptive Ridge Point Refinement for Seeds Detection in X-Ray Coronary Angiogram

    Directory of Open Access Journals (Sweden)

    Ruoxiu Xiao

    2015-01-01

    Full Text Available Seed point is prerequired condition for tracking based method for extracting centerline or vascular structures from the angiogram. In this paper, a novel seed point detection method for coronary artery segmentation is proposed. Vessels on the image are first enhanced according to the distribution of Hessian eigenvalue in multiscale space; consequently, centerlines of tubular vessels are also enhanced. Ridge point is extracted as candidate seed point, which is then refined according to its mathematical definition. The theoretical feasibility of this method is also proven. Finally, all the detected ridge points are checked using a self-adaptive threshold to improve the robustness of results. Clinical angiograms are used to evaluate the performance of the proposed algorithm, and the results show that the proposed algorithm can detect a large set of true seed points located on most branches of vessels. Compared with traditional seed point detection algorithms, the proposed method can detect a larger number of seed points with higher precision. Considering that the proposed method can achieve accurate seed detection without any human interaction, it can be utilized for several clinical applications, such as vessel segmentation, centerline extraction, and topological identification.

  2. The effect of inducing uniform Cu growth on formation of electroless Cu seed layer

    International Nuclear Information System (INIS)

    Lim, Taeho; Kim, Myung Jun; Park, Kyung Ju; Kim, Kwang Hwan; Choe, Seunghoe; Lee, Young-Soo; Kim, Jae Jeong

    2014-01-01

    The uniformity of Cu growth on Pd nanocatalysts was controlled by using organic additives in the formation of electroless Cu seed layers. Polyethylene glycol (PEG, Mw. 8000) not only reduced the deposition rate but also improved the uniformity of Cu growth on each Pd nanocatalyst during the seed layer formation. The stronger suppression effect of PEG on Cu than on Pd reduced the difference in the deposition rate between the two surfaces, resulting in the uniform deposition. Meanwhile, bis(3-sulfopropyl) disulfide degraded the uniformity by strong and nonselective suppression. The sheet resistance measurement and atomic force microscopy imaging revealed that the uniform Cu growth by PEG was more advantageous for the formation of a thin and smooth Cu seed layer than the non-uniform growth. The uniform Cu growth also had a positive influence on the subsequent Cu electrodeposition: the 60-nm-thick electrodeposited Cu film on the Cu seed layer showed low resistivity (2.70 μΩ·cm), low surface roughness (6.98 nm), and good adhesion strength. - Highlights: • Uniform Cu growth on Pd was achieved in formation of electroless Cu seed layer. • PEG addition to electroless bath improved the uniformity of Cu growth on Pd. • A thin, smooth and continuous Cu seed layer was obtained with PEG. • Adhesion strength of the Cu seed layer was also improved with PEG. • The uniformity improvement positively affected subsequent Cu electrodeposition

  3. Repair of Avascular Meniscus Tears with Electrospun Collagen Scaffolds Seeded with Human Cells.

    Science.gov (United States)

    Baek, Jihye; Sovani, Sujata; Glembotski, Nicholas E; Du, Jiang; Jin, Sungho; Grogan, Shawn P; D'Lima, Darryl D

    2016-03-01

    The self-healing capacity of an injured meniscus is limited to the vascularized regions and is especially challenging in the inner avascular regions. As such, we investigated the use of human meniscus cell-seeded electrospun (ES) collagen type I scaffolds to produce meniscal tissue and explored whether these cell-seeded scaffolds can be implanted to repair defects created in meniscal avascular tissue explants. Human meniscal cells (derived from vascular and avascular meniscal tissue) were seeded on ES scaffolds and cultured. Constructs were evaluated for cell viability, gene expression, and mechanical properties. To determine potential for repair of meniscal defects, human meniscus avascular cells were seeded and cultured on aligned ES collagen scaffolds for 4 weeks before implantation. Surgical defects resembling "longitudinal tears" were created in the avascular zone of bovine meniscus and implanted with cell-seeded collagen scaffolds and cultured for 3 weeks. Tissue regeneration and integration were evaluated by histology, immunohistochemistry, mechanical testing, and magentic resonance imaging. Ex vivo implantation with cell-seeded collagen scaffolds resulted in neotissue that was significantly better integrated with the native tissue than acellular collagen scaffolds or untreated defects. Human meniscal cell-seeded ES collagen scaffolds may therefore be useful in facilitating meniscal repair of avascular meniscus tears.

  4. Increasing seed size and quality by manipulating BIG SEEDS1 in legume species.

    Science.gov (United States)

    Ge, Liangfa; Yu, Jianbin; Wang, Hongliang; Luth, Diane; Bai, Guihua; Wang, Kan; Chen, Rujin

    2016-11-01

    Plant organs, such as seeds, are primary sources of food for both humans and animals. Seed size is one of the major agronomic traits that have been selected in crop plants during their domestication. Legume seeds are a major source of dietary proteins and oils. Here, we report a conserved role for the BIG SEEDS1 (BS1) gene in the control of seed size and weight in the model legume Medicago truncatula and the grain legume soybean (Glycine max). BS1 encodes a plant-specific transcription regulator and plays a key role in the control of the size of plant organs, including seeds, seed pods, and leaves, through a regulatory module that targets primary cell proliferation. Importantly, down-regulation of BS1 orthologs in soybean by an artificial microRNA significantly increased soybean seed size, weight, and amino acid content. Our results provide a strategy for the increase in yield and seed quality in legumes.

  5. Mycoflora in Exhumed Seeds of Opuntia tomentosa and Its Possible Role in Seed Germination

    Directory of Open Access Journals (Sweden)

    María Esther Sánchez-Coronado

    2011-01-01

    Full Text Available The funicular cover of the Opuntia tomentosa seed limits imbibition; germination occurs only when the funicle is weakened or the funicular valve is removed. We investigated the role of fungi in funicular weakening and seed germination. Seeds that had been either buried in one of two sites or stored in the laboratory were germinated with and without a valve. Disinfected or nondisinfected seeds and their naked embryos were cultivated on agar or PDA. None of the 11 identified fungal genera grew on the disinfected control seeds or the embryos. The mycoflora present on disinfected and nondisinfected exhumed seeds suggest that the fungal colonization occurred in the soil and differed between the burial sites. Exhumed seeds with and without a valve germinated in high percentages, whereas only the control seeds without a valve germinated. Scanning electron micrographs showed that the hyphae penetrated, cracked, and eroded the funicular envelope of exhumed seeds.

  6. Reduction of microbial contamination and improvement of germination of sweet basil (Ocimum basilicum L.) seeds via surface dielectric barrier discharge

    Science.gov (United States)

    Ambrico, Paolo F.; Šimek, Milan; Morano, Massimo; De Miccolis Angelini, Rita M.; Minafra, Angelantonio; Trotti, Pasquale; Ambrico, Marianna; Prukner, Václav; Faretra, Francesco

    2017-08-01

    Naturally contaminated basil seeds were treated by a surface dielectric barrier discharge driven in the humid air by an amplitude modulated AC high voltage to avoid heat shock. In order to avoid direct contact of seeds with microdischarge filaments, the seeds to be treated were placed at sufficient distance from the surface discharge. After treatment, the seeds were analyzed in comparison with control samples for their microbial contamination as well as for the capability of germination and seedling growth. Moreover, chemical modification of seed surface was observed through the elemental energy dispersive x-ray analysis and wettability tests. We found that treatment applied at 20% duty cycle (effective discharge duration up to 20 s) significantly decreases microbial load without reducing the viability of the seeds. On the other side, seedling growth was considerably accelerated after the treatment, and biometric growth parameters of seedlings (total length, weight, leaf extension) considerably increased compared to the controls. Interestingly, scanning electron microscopy images taken for the different duration of treatment revealed that seed radicle micropylar regions underwent significant morphological changes while the coat was substantially undamaged. Inside the seed, the embryo seemed to be well preserved while the endosperm body was detached from the epithelial tegument. A total of 9 different genera of fungi were recovered from the analyzed seeds. Scanning electron microscopy images revealed that conidia were localized especially in the micropylar region, and after plasma treatment, most of them showed substantial damages. Therefore, the overall effect of the treatment of naturally contaminated seeds by reactive oxygen and nitrogen species produced by plasma and the consequent changes in surface chemistry and microbial load can significantly improve seed vigor.

  7. Reduction of microbial contamination and improvement of germination of sweet basil ( Ocimum basilicum L.) seeds via surface dielectric barrier discharge

    International Nuclear Information System (INIS)

    Ambrico, Paolo F; Ambrico, Marianna; Šimek, Milan; Prukner, Václav; Morano, Massimo; De Miccolis Angelini, Rita M; Trotti, Pasquale; Faretra, Francesco; Minafra, Angelantonio

    2017-01-01

    Naturally contaminated basil seeds were treated by a surface dielectric barrier discharge driven in the humid air by an amplitude modulated AC high voltage to avoid heat shock. In order to avoid direct contact of seeds with microdischarge filaments, the seeds to be treated were placed at sufficient distance from the surface discharge. After treatment, the seeds were analyzed in comparison with control samples for their microbial contamination as well as for the capability of germination and seedling growth. Moreover, chemical modification of seed surface was observed through the elemental energy dispersive x-ray analysis and wettability tests. We found that treatment applied at 20% duty cycle (effective discharge duration up to 20 s) significantly decreases microbial load without reducing the viability of the seeds. On the other side, seedling growth was considerably accelerated after the treatment, and biometric growth parameters of seedlings (total length, weight, leaf extension) considerably increased compared to the controls. Interestingly, scanning electron microscopy images taken for the different duration of treatment revealed that seed radicle micropylar regions underwent significant morphological changes while the coat was substantially undamaged. Inside the seed, the embryo seemed to be well preserved while the endosperm body was detached from the epithelial tegument. A total of 9 different genera of fungi were recovered from the analyzed seeds. Scanning electron microscopy images revealed that conidia were localized especially in the micropylar region, and after plasma treatment, most of them showed substantial damages. Therefore, the overall effect of the treatment of naturally contaminated seeds by reactive oxygen and nitrogen species produced by plasma and the consequent changes in surface chemistry and microbial load can significantly improve seed vigor. (paper)

  8. Agrowaste: Phytosterol from Durian Seed

    OpenAIRE

    D. Mohd Nazrul Hisham; J. Mohd Lip; R. Suri; H. Mohamed Shafit; Z.Kharis; K. Shazlin; A. Normah; M.F. Nurul Nabilah

    2012-01-01

    Presence of phytosterol compound in Durian seed (Durio zibethinus) or known as King of fruits has been discovered from screening work using reagent test. Further analysis work has been carried out using mass spectrometer in order to support the priliminary finding. Isolation and purification of the major phytosterol has been carried out using an open column chromatography. The separation was monitored using thin layer chromatography (TLC). Major isolated compounds and pur...

  9. Preplanting irradiation of tomato seeds

    International Nuclear Information System (INIS)

    Maltseva, S.

    1976-01-01

    Seeds of the tomato varieties Pioneer-2, Drouzhba and Ace were treated prior to planting with Co 60 gamma rays in optimal doses of 2000 R and the varieties No 10 x Bison, Triumph and Extase with 1500 R. This treatment raised the germination energy and the plants started flowering and ripening earlier. The index of earliness was enhanced but the overall yield was equal to that of the control plants. (author)

  10. Proteomics of Rice Seed Germination

    Directory of Open Access Journals (Sweden)

    Dongli eHe

    2013-07-01

    Full Text Available Seed is a condensed form of plant. Under suitable environmental conditions, it can resume the metabolic activity from physiological quiescent status, and mobilize the reserves, biosynthesize new proteins, regenerate organelles and cell membrane, eventually protrude the radicle and enter into seedling establishment. So far, how these activities are regulated in a coordinated and sequential manner is largely unknown. With the availability of more and more genome sequence information and the development of mass spectrometry (MS technology, proteomics has been widely applied in analyzing the mechanisms of different biological processes, and proved to be very powerful. Regulation of rice seed germination is critical for rice cultivation. In recent years, a lot of proteomic studies have been conducted in exploring the gene expression regulation, reserves mobilization and metabolisms reactivation, which brings us new insights on the mechanisms of metabolism regulation during this process. Nevertheless, it also invokes a lot of questions. In this mini-review, we summarized the progress in the proteomic studies of rice seed germination. The current challenges and future perspectives were also discussed, which might be helpful for the following studies.

  11. PHO1 Exports Phosphate from the Chalazal Seed Coat to the Embryo in Developing Arabidopsis Seeds.

    Science.gov (United States)

    Vogiatzaki, Evangelia; Baroux, Célia; Jung, Ji-Yul; Poirier, Yves

    2017-10-09

    Seed production requires the transfer of nutrients from the maternal seed coat to the filial endosperm and embryo. Because seed coat and filial tissues are symplasmically isolated, nutrients arriving in the seed coat via the phloem must be exported to the apoplast before reaching the embryo. Proteins implicated in the transfer of inorganic phosphate (Pi) from the seed coat to the embryo are unknown despite seed P content being an important agronomic trait. Here we show that the Arabidopsis Pi exporters PHO1 and PHOH1 are expressed in the chalazal seed coat (CZSC) of developing seeds. PHO1 is additionally expressed in developing ovules. Phosphorus (P) content and Pi flux between the seed coat and embryo were analyzed in seeds from grafts between WT roots and scions from either pho1, phoh1, or the pho1 phoh1 double mutant. Whereas P content and distribution between the seed coat and embryo in fully mature dry seeds of these mutants are similar to the WT, at the mature green stage of seed development the seed coat of the pho1 and pho1 phoh1 mutants, but not of the phoh1 mutant, retains approximately 2-fold more P than its WT control. Expression of PHO1 under a CZSC-specific promoter complemented the seed P distribution phenotype of the pho1 phoh1 double mutant. CZSC-specific down-expression of PHO1 also recapitulated the seed P distribution phenotype of pho1. Together, these experiments show that PHO1 expression in the CZSC is important for the transfer of P from the seed coat to the embryo in developing seeds. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Application of 125I seed permanent plantation in osseous metastases

    International Nuclear Information System (INIS)

    Zhang Fujun; Wu Peihong; Lu Mingjian; Li Kui; Zhang Liang; Huang Jinhua; Fan Weijun; Zhao Ming; Gu Yangkui; Liu Jian; Wang Junjie

    2007-01-01

    Objective: To evaluate the value of 125 I permanent plantation in treating osseous metastases. Methods: Twenty-two patients with osseous metastases were accepted radioactive seeds 125 I permanent plantation. The curative effect was appraised according to the degree of ostalgia relieving and the changing of the radiology imaging in patients. Results: Accepted radioactive seeds 125 I permanent plantation, relief of pain was obtained and the effective rate is 91% (20/22). However none of the patients showed severe side-effect. Among 32 lesions in 22 cases followed-up by CT in 2 months, 4 obtained CR, 18 obtained PR, 10 NC and 0 PD. The responsive rate was 68.7%. Conclusion: 125 I permanent plantation procedure can be a safe and effective method in treating osseous metastases and obtaining good clinical effects with minimal damage and few complications. (authors)

  13. Image, Image, Image

    Science.gov (United States)

    Howell, Robert T.

    2004-01-01

    With all the talk today about accountability, budget cuts, and the closing of programs in public education, teachers cannot overlook the importance of image in the field of industrial technology. It is very easy for administrators to cut ITE (industrial technology education) programs to save school money--money they might shift to teaching the…

  14. Clinical application of permanent implantation of iodine 125 seeds for osseous metastases

    International Nuclear Information System (INIS)

    Li Jinxin; Zhang Qizhou; Li Guoliang

    2010-01-01

    Objective: To evaluate the clinical efficacy of permanent implantation of iodine 125 seeds in the treatment of osseous metastases. Methods: Radioactive iodine 125 seeds were implanted permanently in 32 lesions of 25 patients with osseous metastases of different origins. The ostalgia-relieving degree and the imaging alterations of the osseous metastasis lesions were observed. Results: The effective pain-relieving rate was 92% caused by permanent implantation of iodine 125 seeds. Of al 1 the patients, 15 patients achieved complete response, 8 patients obtained partial response (PR), and 2 patients had no change. The pain grade was decreased significantly after the treatment (P 125 I seeds has a definite effect on tumor metastasis and caused minimal damage and few complications. It is worthy of popularization in clinic. (authors)

  15. Seed Mediated Growth of Gold Nanoparticles Based on Liquid Arc Discharge

    International Nuclear Information System (INIS)

    Ashkarran, Ali Akbar

    2013-01-01

    We report studies on the growth of gold nanoparticles by a seed-mediated approach in solution. The synthetic method is adapted from one we published earlier (Ashkarran et al. Appl. Phys. A 2009, 96, 423). The synthesized gold nanoparticles were characterized by X-ray diffraction (XRD), dynamic light scattering (DLS), UV-Vis spectroscopy, optical imaging and atomic force microscopy (AFM). Optical absorption spectroscopy of the prepared samples at 15 A arc current in HAuCl 4 solution shows a surface plasmon resonance around 520 nm. It is found that sodium citrate acts as a stabilizer and surface capping agent of the colloidal nanoparticles. The intensity of the plasmonic peak of the prepared gold nanoparticles for 1 minute arc duration gradually increases due to seed mediation for up to 6 hours. The formation time of gold nanoparticles at higher seed concentrations is less than that at lower seed concentrations. (plasma technology)

  16. Radioactive seed immobilization techniques for interstitial brachytherapy

    International Nuclear Information System (INIS)

    Yan, K.; Podder, T.; Buzurovic, I.; Hu, Y.; Dicker, A.; Valicenti, R.; Yu, Y.; Messing, E.; Rubens, D.; Sarkar, N.; Ng, W.

    2008-01-01

    In prostate brachytherapy, seeds can detach from their deposited sites and move locally in the pelvis or migrate to distant sites including the pulmonary and cardiac regions. Undesirable consequences of seed migration include inadequate dose coverage of the prostate and tissue irradiation effects at the site of migration. Thus, it is clinically important to develop seed immobilization techniques. We first analyze the possible causes for seed movement, and propose three potential techniques for seed immobilization: (1) surgical glue, (2) laser coagulation and (3) diathermy coagulation. The feasibility of each method is explored. Experiments were carried out using fresh bovine livers to investigate the efficacy of seed immobilization using surgical glue. Results have shown that the surgical glue can effectively immobilize the seeds. Evaluation of the radiation dose distribution revealed that the non-immobilized seed movement would change the planned isodose distribution considerably; while by using surgical glue method to immobilize the seeds, the changes were negligible. Prostate brachytherapy seed immobilization is necessary and three alternative mechanisms are promising for addressing this issue. Experiments for exploring the efficacy of the other two proposed methods are ongoing. Devices compatible with the brachytherapy procedure will be designed in future. (orig.)

  17. Partial dehydration and cryopreservation of Citrus seeds.

    Science.gov (United States)

    Graiver, Natalia; Califano, Alicia; Zaritzky, Noemí

    2011-11-01

    Three categories of seed storage behavior are generally recognized among plant species: orthodox, intermediate and recalcitrant. Intermediate seeds cannot be stored in liquid nitrogen (LN) without a previous partial dehydration process. The water content (WC) of the seeds at the moment of immersion in LN must be regarded as the most critical factor in cryopreservation. The purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of Citrus seeds: C. sinensis (sweet orange), C. paradisi (grapefruit), C. reticulata (mandarin) in LN. To study the tolerance to dehydration and LN exposure, seeds were desiccated by equilibration at relative humidities between 11 and 95%. Sorption isotherms were determined and modeled; lipid content of the seeds was measured. Seed desiccation sensitivity was quantified by the quantal response model. Differential scanning calorimetry (DSC) thermograms were determined on cotyledon tissue at different moisture contents to measure ice melting enthalpies and unfrozen WC. Samples of total seed lipid extract were also analyzed by DSC to identify lipid transitions in the thermograms. The limit of hydration for LN Citrus seeds treatment corresponded to the unfrozen WC in the tissue, confirming that seed survival strictly depended on avoidance of intracellular ice formation. Copyright © 2011 Society of Chemical Industry.

  18. Electrophysiology of pumpkin seeds: Memristors in vivo.

    Science.gov (United States)

    Volkov, Alexander G; Nyasani, Eunice K; Tuckett, Clayton; Greeman, Esther A; Markin, Vladislav S

    2016-01-01

    Leon Chua, the discoverer of a memristor, theoretically predicted that voltage gated ion channels can be memristors. We recently found memristors in different plants such as the Venus flytrap, Mimosa pudica, Aloe vera, apple fruits, and in potato tubers. There are no publications in literature about the existence of memristors in seeds. The goal of this work was to discover if pumpkin seeds might have memristors. We selected Cucurbita pepo L., cv. Cinderella, Cucurbita maxima L. cv Warty Goblin, and Cucurbita maxima L., cv. Jarrahdale seeds for this analysis. In these seeds, we found the presence of resistors with memory. The analysis was based on cyclic voltammetry where a memristor should manifest itself as a nonlinear two-terminal electrical element, which exhibits a pinched hysteresis loop on a current-voltage plane for any bipolar cyclic voltage input signal. Dry dormant pumpkin seeds have very high electrical resistance without memristive properties. The electrostimulation by bipolar sinusoidal or triangular periodic waves induces electrical responses in imbibed pumpkin seeds with fingerprints of memristors. Tetraethylammonium chloride, an inhibitor of voltage gated K(+) channels, transforms a memristor to a resistor in pumpkin seeds. NPPB (5-Nitro-2-(3-phenylpropylamino)benzoic acid) inhibits the memristive properties of imbibed pumpkin seeds. The discovery of memristors in pumpkin seeds creates a new direction in the understanding of electrophysiological phenomena in seeds.

  19. Germination of beans and snap beans seed

    Directory of Open Access Journals (Sweden)

    Zdravković Milan

    2000-01-01

    Full Text Available The aim of this study was to investigate germination of good bean seed of the variety Galeb and the bad bean seed of the same variety. We were also interested in germination of bean and snap bean seed damaged by grain weevil, and in germination of the seed treated by freezing which was aimed at controlling grain weevil by cold. We also recorded the differences between bean and snap bean seed, which was or was not treated by freezing in laboratory conditions. This investigation was carried out by applying the two factorial block system. The obtained results were evaluated by the variance analysis and x2 test These results suggest that the bean seed of a bad fraction had low levels of germination, but still it was present. Although the seed of good appearance was carefully selected, germination was slightly lower than it should have been. The seed with the large amount of grain weevils performed a high level germination in laboratory conditions. There were no differences in germination between the seed injured by grain weevil either in beans or in snap beans. As for the seed treated or untreated by freezing, there also were no differences between beans and snap beans. .

  20. Local evolution of seed flotation in Arabidopsis.

    Directory of Open Access Journals (Sweden)

    Susana Saez-Aguayo

    2014-03-01

    Full Text Available Arabidopsis seeds rapidly release hydrophilic polysaccharides from the seed coat on imbibition. These form a heavy mucilage layer around the seed that makes it sink in water. Fourteen natural Arabidopsis variants from central Asia and Scandinavia were identified with seeds that have modified mucilage release and float. Four of these have a novel mucilage phenotype with almost none of the released mucilage adhering to the seed and the absence of cellulose microfibrils. Mucilage release was modified in the variants by ten independent causal mutations in four different loci. Seven distinct mutations affected one locus, coding the MUM2 β-D-galactosidase, and represent a striking example of allelic heterogeneity. The modification of mucilage release has thus evolved a number of times independently in two restricted geographical zones. All the natural mutants identified still accumulated mucilage polysaccharides in seed coat epidermal cells. Using nuclear magnetic resonance (NMR relaxometry their production and retention was shown to reduce water mobility into internal seed tissues during imbibition, which would help to maintain seed buoyancy. Surprisingly, despite released mucilage being an excellent hydrogel it did not increase the rate of water uptake by internal seed tissues and is more likely to play a role in retaining water around the seed.

  1. Selected aspects of tiny vetch [Vicia hirsuta (L. Gray S.F.] seed ecology: generative reproduction and effects of seed maturity and seed storage on seed germination

    Directory of Open Access Journals (Sweden)

    Magdalena Kucewicz

    2012-12-01

    Full Text Available Vicia hirsuta (L. Gray S.F. (tiny vetch is a common and persistent segetal weed. Tiny vetch seeds and pods reach different stages of maturity during the crop harvest season. Some seeds that mature before cereal harvest are shed in the field and deposited in the soil seed bank, while others become incorporated into seed material. The objective of this study was to describe selected aspects of tiny vetch seed ecology: to determine the rate of individual reproduction of vetch plants growing in winter and spring grain crops and to evaluate the germination of seeds at different stages of maturity, subject to storage conditions. The seeds and pods of V. hirsuta were sorted according to their development stages at harvest and divided into two groups. The first group was stored under laboratory conditions for two months. In the autumn of the same year, the seeds were subjected to germination tests. The remaining seeds were stored in a storeroom, and were planted in soil in the spring. The germination rate was evaluated after 8 months of storage. Potential productivity (developed pods and flowers, fruit buds was higher in plants fruiting in winter wheat than in spring barley. Vetch plants produced around 17-26% more pods (including cracked, mature, greenish-brown and green pods and around 25% less buds in winter wheat than in spring barley. Immature seeds were characterized by the highest germination capacity. Following storage under laboratory conditions and stratification in soil, mature seeds germinated at a rate of several percent. After storage in a storeroom, seeds at all three development stages broke dormancy at a rate of 72- 75%. The high germination power of tiny vetch seeds stored in a storeroom indicates that this plant can be classified as an obligatory speirochoric weed species.

  2. Impurities in alfalfa seed and their impact on processing technology

    OpenAIRE

    Đokić, Dragoslav; Stanisavljević, Rade; Marković, Jordan; Terzić, Dragan; Anđelković, Bojan

    2012-01-01

    The aim of this research was to determine relevant parameters and optimal alfalfa seed processing technology by a comparative analysis of two systems of machinery for processing natural alfalfa seed of different purity (59.0% and 71.0%) and with different content of impurities. The relevant parameters monitored during the test were: pure seed (%), weed seeds and seeds of other crops (%), inert matter (%), amount of processed seed (kg), seed processing time (min), consumption of steel powder (...

  3. Radio-guided occult lesion localisation using iodine-125 seeds ('ROLLIS') for removal of impalpable breast lesions: first Australian experience

    International Nuclear Information System (INIS)

    Taylor, Donna B.; Bourke, Anita G.; Westcott, Eliza

    2015-01-01

    Approximately one-third of breast cancers are impalpable and require pre-operative image-guided localisation. Hook-wire localisation (HWL) is commonly used but has several disadvantages. Use of a low-activity radioactive iodine-125 seed is a promising alternative technique used in the USA and the Netherlands. This pilot study describes the first use of this in Australia. In this prospective pilot study, 21 participants with biopsy-proven breast cancer underwent radio guided occult lesion localisation using iodine-125 seed(s) (ROLLIS) with insertion of a hook-wire for back up. Sentinel node biopsy was performed where indicated. Ease of hook-wire and seed insertion, duration of the procedure, dependence on the seed versus hook-wire during surgery, lesion location within the specimen, histopathology including size of radial margins, the ease of seed retrieval in pathology, and safe return of seeds for disposal were documented. Radiation dosimetry of staff was performed. All seeds were placed within 3.5 mm of the lesion. All lesions and seeds were removed. One participant needed re-excision for involved margins. Radiologists and surgeons both preferred ROLLIS. Surgeons were able to depend on the seed for localisation in all but one case. Sentinel node biopsy was successfully performed when required. Pathologists found seed retrieval quick and easy, with no detrimental effect on tissue processing. No radiation doses measurably above background were received by staff. ROLLIS is an easily learnt, safe and effective alternative technique to standard HWL.

  4. Effects of rodent species, seed species, and predator cues on seed fate

    Science.gov (United States)

    Sivy, Kelly J.; Ostoja, Steven M.; Schupp, Eugene W.; Durham, Susan

    2011-07-01

    Seed selection, removal and subsequent management by granivorous animals is thought to be a complex interaction of factors including qualities of the seeds themselves (e.g., seed size, nutritional quality) and features of the local habitat (e.g. perceived predator risk). At the same time, differential seed selection and dispersal is thought to have profound effects on seed fate and potentially vegetation dynamics. In a feeding arena, we tested whether rodent species, seed species, and indirect and direct predation cues influence seed selection and handling behaviors (e.g., scatter hoarding versus larder hoarding) of two heteromyid rodents, Ord's kangaroo rat ( Dipodomys ordii) and the Great Basin pocket mouse ( Perognathus parvus). The indirect cue was shrub cover, a feature of the environment. Direct cues, presented individually, were (1) control, (2) coyote ( Canis latrans) vocalization, (3) coyote scent, (4) red fox ( Vulpes vulpes) scent, or (5) short-eared owl ( Asio flammeus) vocalization. We offered seeds of three sizes: two native grasses, Indian ricegrass ( Achnatherum hymenoides) and bluebunch wheatgrass ( Pseudoroegneria spicata), and the non-native cereal rye ( Secale cereale), each in separate trays. Kangaroo rats preferentially harvested Indian ricegrass while pocket mice predominately harvested Indian ricegrass and cereal rye. Pocket mice were more likely to scatter hoard preferred seeds, whereas kangaroo rats mostly consumed and/or larder hoarded preferred seeds. No predator cue significantly affected seed preferences. However, both species altered seed handling behavior in response to direct predation cues by leaving more seeds available in the seed pool, though they responded to different predator cues. If these results translate to natural dynamics on the landscape, the two rodents are expected to have different impacts on seed survival and plant recruitment via their different seed selection and seed handling behaviors.

  5. Seed source, seed traits, and frugivore habits: Implications for dispersal quality of two sympatric primates.

    Science.gov (United States)

    Benítez-Malvido, Julieta; González-Di Pierro, Ana Ma; Lombera, Rafael; Guillén, Susana; Estrada, Alejandro

    2014-06-01

    • Premise of the study: Frugivore selection of fruits and treatment of seeds together with seed deposition site are crucial for the population dynamics of vertebrate-dispersed plants. However, frugivore species may influence dispersal quality differently even when feeding on the same fruit species and, while animals disperse some seeds, others simply fall beneath the parent plant.• Methods: In southern Mexico, we investigated to see if within-species seed traits (i.e., length, width, weight, and volume) and germination success differed according to seed source. For five tropical tree species we obtained ingested seeds from two sources, howler monkey (Alouatta pigra) and spider monkey (Ateles geoffroyi) feces; and noningested seeds from two sources, the ground and tree crowns (with predispersed seeds used as control).• Key results: A principal components' analysis showed that traits of seeds ingested by howler monkeys differed from other sources while seeds ingested by spider monkeys were similar to noningested seeds. Howlers consumed on average the larger seeds in Ampelocera hottlei, Brosimum lactescens, and Dialium guianense. Both primate species consumed the smaller seeds in Spondias mombin, while no seed trait differences among seed sources were found in Spondias radlkoferi. For all five tree species, germination rate was greatest for seeds ingested by howler monkeys.• Conclusions: For the studied plant species, seed ingestion by howler monkeys confers higher dispersal quality than ingestion by spider monkeys or nondispersal. Dispersal services of both primate species, however, are not redundant and may contribute to germination heterogeneity within plant populations in tropical forests. © 2014 Botanical Society of America, Inc.

  6. Yield and quality of white mustard seed depending on the seeding dates

    Directory of Open Access Journals (Sweden)

    Jevđović Radosav

    2000-01-01

    Full Text Available The effect of sowing dates and harvest dates on yield and quality of white mustard seed been investigated in this study. During the experiment two sowing dates have been tested the first one based on the crops in March, and the other one when the direct sowing was carried out in late May, after the harvest of camomile crops. The harvest of the seed crops in the first seeding date was performed on 1st July, and the second on 15th September. Seed fractioning extracted the following fractions: large (3mm, medium (2 mm and small (1mm. I he objective of the laboratory' experiments was to observe the effect of the seed fraction size on the most important parameters of its quality (germination energy, total germination and absolute mass according to the stipulated terms. Based on the data obtained in the survey, it can be concluded that the seed yield is significantly affected by the varied seeding dates. Earlier seeding date produced significantly increased seed yield (1920 kg/ha than the later seeding date (1160 kg/ha. According to the seed quality parameters, the best characteristics are found in the seed fraction of 3 mm, in both seeding and harvest dates.

  7. Efeito da idade e do repouso pós-colheita de frutos de pepino na semente armazenada Effect of fruit age and post-harvest period of cucumber on stored seeds

    Directory of Open Access Journals (Sweden)

    Claudio José Barbedo

    1999-05-01

    Full Text Available Para avaliar os efeitos da idade e do repouso pós-colheita de frutos de pepino (Cucumis sativus L. de duas cultivares, Pérola (espinhos escuros e Rubi (espinhos claros, sobre as alterações na qualidade fisiológica das sementes durante o armazenamento, conduziu-se um experimento em Botucatu, SP e Bandeirantes, PR, durante quatro anos. Os frutos foram colhidos desde os 15 ('Rubi' ou 20 ('Pérola' dias até 45 dias após a antese, permanecendo em repouso pós-colheita por 0, 5, 10 e 15 dias, antes da extração das sementes. Antes e após dois e três anos de armazenamento (sacos de papel em ambiente de laboratório, foram retiradas amostras para avaliação do potencial germinativo e do vigor. Os resultados revelaram que sementes imaturas, retiradas de frutos colhidos precocemente (até 30 dias após a antese e sem repouso pós-colheita, perderam totalmente a capacidade germinativa no segundo ano de armazenamento. Sementes que apresentavam valores iniciais de germinação mais elevados, as colhidas de frutos maduros (com ou sem repouso pós-colheita ou de imaturos (30 e 35 dias de idade com 10 a 15 dias de repouso pós-colheita, mantiveram valores acima de 70% após três anos de armazenamento.Seeds from two cucumber (Cucumis sativus L. cultivars, Pérola and Rubi, were evaluated in relation to physiological quality to verify the effects of age and postharvest period of fruits on seed storage potential. Fruits were harvested from 15 ('Rubi' or 20 ('Pérola' to 45 days after flowering and mantained in postharvest for 0, 5, 10, and 15 days before seeds extraction, at room conditions. Seeds were dried and stored in paper bags at room conditions for two and three years. Before and after the storage periods, samples were taken to germination and vigour tests. Results showed that immature seeds (extracted from immature fruits - 30 days after flowering without post-harvest period, did not germinate at second year. However, seeds with high

  8. Activity uniformity of Ir-192 seeds

    International Nuclear Information System (INIS)

    Ling, C.C.; Gromadzki, Z.C.

    1981-01-01

    A simple device that uses materials and apparatus commonly available in a radiotherapy department has been designed, fabricated and used in routine quality control relative to the activity uniformity of clinical Ir-192 seeds in ribbons. Detailed evaluation indicated that this system is easy to use and can yield relative activity measurements of individual Ir-192 seeds accurate to within 2%. With this device, activity uniformity of commercial Ir-192 seeds from two manufacturers has been assessed. For the seven shipments of Ir-192 seeds studied, the root mean square variations of individual seed strength from the average of each shipment ranged from 3.4 to 7.1%. Variation in seed activity by more than +- 10% from the average is not uncommon

  9. Commercial Scale Production of Mushroom Liquid Seeds

    International Nuclear Information System (INIS)

    Rosnani Abdul Rashid; Hassan Hamdani Hassan Mutaat; Mohd Meswan Maskom; Khairuddin Abdul Rahim

    2015-01-01

    Mushroom liquid seed production technology was developed by Malaysian Nuclear Agency (Nuclear Malaysia) in the late 1990s. Initially, the liquid seeds were used mainly in the solid state fermentation process for converting oil palm empty fruit bunch fibres into ruminant feed. Considering widespread problems encountered by mushroom growers from use of solid seeds, especially in cases of contaminant agents infecting cultivated bags and inconsistencies in yield, we diverted our focus to utilising liquid seeds as alternative inocula for mushroom cultivation. These problems provide us opportunities to look into the issues and address the problems faced by mushroom growers. However, the technology of producing liquid seed at laboratory scale needs to be primed for commercial production. This paper discusses developmental aspects of mushroom liquid seed at commercial scale for the advancement of the country's mushroom industry. (author)

  10. Chemical solution seed layer for rabits tapes

    Science.gov (United States)

    Goyal, Amit; Paranthaman, Mariappan; Wee, Sung-Hun

    2014-06-10

    A method for making a superconducting article includes the steps of providing a biaxially textured substrate. A seed layer is then deposited. The seed layer includes a double perovskite of the formula A.sub.2B'B''O.sub.6, where A is rare earth or alkaline earth metal and B' and B'' are different rare earth or transition metal cations. A superconductor layer is grown epitaxially such that the superconductor layer is supported by the seed layer.

  11. Effects of seed priming and water potential on seed germination and ...

    African Journals Online (AJOL)

    Administrator

    2011-05-23

    May 23, 2011 ... (without seed drying), primed and 12 h drying on seed germination of wheat .... completely with the lower half and the paper were rolled and placed ..... water stress and control of germination: a review. Plant Cell Environ.

  12. [Seed geography: its concept and basic scientific issues].

    Science.gov (United States)

    Yu, Shun-Li; Wang, Zong-Shuai; Zeren, Wangmu

    2010-01-01

    In this paper, a new concept 'seed geography' was provided, and its definition, research contents, and scientific issues were put forward. Seed geography is a newly developed interdisciplinary science from plant geography, seed ecology, and phytosociology, which studies the geographic variation patterns of seed biological traits as well as their relationships with environmental factors from macroscopic to microscopic, and the seed formation, development, and change trends. The main research contents would include geography of seed mass, geography of seed chemical components, geography of seed morphology, geography of seed cell biological characteristics, geography of seed physiological characteristics, geography of seed genetic characteristics, and geography of flower and fruit. To explore the scientific issues in seed geography would help us to better understand the long-term adaptation and evolution of seed characteristics to natural environments.

  13. [The research of Valeriana amurensis seed germination characteristics].

    Science.gov (United States)

    Liu, Juan; Yang, Chun-Rong; Jiang, Bo; Fang, Min; Du, Juan

    2011-10-01

    To study the effect of different treatments on the Valeriana amurensis seed germination rate. Used different chemical reagents and seed soakings on the routine germination test and the orthogonal test of the Valeriana amurensis seed, calculated the germination rate under different germination condition. Valeriana amurensis treated with different chemical reagends had different germination rate. The suitable immersion time could enhance Valeriana amurensis seed germination rate. Different treatment time, different disposal temperature, different germination temperature would have an impact on the Valeriana amurensis seed germination rate. In order to raise the Valeriana amurensis seed germination rate, use appropriate treatment on the seed before plant seeds; The seed growing must under suitable time and temperature.

  14. New design of experiment combined with UV-Vis spectroscopy for extraction and estimation of polyphenols from Basil seeds, Red seeds, Sesame seeds and Ajwan seeds

    Science.gov (United States)

    Mabood, Fazal; Gilani, Syed Abdullah; Hussain, Javid; Alshidani, Sulaiman; Alghawi, Said; Albroumi, Mohammed; Alameri, Saif; Jabeen, Farah; Hussain, Zahid; Al-Harrasi, Ahmed; Al Abri, Zahra K. M.; Farooq, Saima; Naureen, Zakira; Hamaed, Ahmad; Rasul Jan, M.; Shah, Jasmin

    2017-05-01

    New experimental designs for the extraction of polyphenols from different seeds including Basil seed, Red seed, Sesame seeds and Ajwan seeds were investigated. Four variables the concentration and volume of methanol and NaOH solutions as well as the temperature and time of extraction were varied to see their effect on total phenol extraction. The temperature was varied in the range from 25 °C to 200 °C while the time in the range from 30 to 200 minutes. Response surface methodology was used to optimize the extraction parameters. The estimation of polyphenols was measured through phenols reduction UV-Vis spectroscopic method of phosphotungstic-phosphomolybdic acids (Folin-Ciocalteu's reagent). Calibration curve was made by using tannic acid as a polyphenols standard in the concentration range from 0.1 to 10 ppm. The regression line obtained shows the value of correlation coefficient i.e. R = 0.930 and Root mean square error of cross validation (RMSEC) value of 0.0654. The Basil seeds were found containing the highest amount of total phenols i.e. 785.76 mg/100 g. While the Sesame seeds having the least amount i.e. 33.08 mg/100 g. The Ajwan seeds and the Red seeds are containing the medium amounts i.e. 379 mg/100 g and 220.54 mg/100 g respectively.

  15. Regulatory redox state in tree seeds

    Directory of Open Access Journals (Sweden)

    Ewelina Ratajczak

    2017-12-01

    Full Text Available Peroxiredoxins (Prx are important regulators of the redox status of tree seeds during maturation and long-term storage. Thioredoxins (Trx are redox transmitters and thereby regulate Prx activity. Current research is focused on the association of Trx with Prx in tree seeds differing in the tolerance to desiccation. The results will allow for better understanding the regulation of the redox status in orthodox, recalcitrant, and intermediate seeds. The findings will also elucidate the role of the redox status during the loss of viability of sensitive seeds during drying and long-term storage.

  16. New design of experiment combined with UV-Vis spectroscopy for extraction and estimation of polyphenols from Basil seeds, Red seeds, Sesame seeds and Ajwan seeds.

    Science.gov (United States)

    Mabood, Fazal; Gilani, Syed Abdullah; Hussain, Javid; Alshidani, Sulaiman; Alghawi, Said; Albroumi, Mohammed; Alameri, Saif; Jabeen, Farah; Hussain, Zahid; Al-Harrasi, Ahmed; Al Abri, Zahra K M; Farooq, Saima; Naureen, Zakira; Hamaed, Ahmad; Rasul Jan, M; Shah, Jasmin

    2017-05-05

    New experimental designs for the extraction of polyphenols from different seeds including Basil seed, Red seed, Sesame seeds and Ajwan seeds were investigated. Four variables the concentration and volume of methanol and NaOH solutions as well as the temperature and time of extraction were varied to see their effect on total phenol extraction. The temperature was varied in the range from 25°C to 200°C while the time in the range from 30 to 200minutes. Response surface methodology was used to optimize the extraction parameters. The estimation of polyphenols was measured through phenols reduction UV-Vis spectroscopic method of phosphotungstic-phosphomolybdic acids (Folin-Ciocalteu's reagent). Calibration curve was made by using tannic acid as a polyphenols standard in the concentration range from 0.1 to 10ppm. The regression line obtained shows the value of correlation coefficient i.e. R=0.930 and Root mean square error of cross validation (RMSEC) value of 0.0654. The Basil seeds were found containing the highest amount of total phenols i.e. 785.76mg/100g. While the Sesame seeds having the least amount i.e. 33.08mg/100g. The Ajwan seeds and the Red seeds are containing the medium amounts i.e. 379mg/100g and 220.54mg/100g respectively. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. Patogenic fungi associated with blue lupine seeds

    Directory of Open Access Journals (Sweden)

    Bogdan Nowicki

    2013-12-01

    Full Text Available Over 10% ofseeds harvested in 1991 and 1992 (50 samples, 400 seeds in each sample proved to be infested with various fungi. Fusarium spp. and Botrytis cinerea were the most common pathogens isolated. Fusarium avenaceum was the most common and highIy pathogenic species. Fusarium semitectum and F. tricinctum were highly pathogenic to lupin seedlings but they were the least common Fusarium isolated from seeds. Similarily, Sclerotinia sclerotiorum was isolated only from 0,2% seeds tested but this fungus was highly pathogenic to lupin seedlings. Some other fungi know as lupin pathogens (F. oxysporum, Stemphylium botryosum, Pleiochaeta setosa and Phomopsis leptostromiformis were also noted in tested seeds.

  18. Occurrence of root parsley pathogens inhabiting seeds

    Directory of Open Access Journals (Sweden)

    Bogdan Nowicki

    2013-12-01

    Full Text Available The studies on root parsley pathogens inhabiting seeds were conducted during 1981-1988 and in 1993. Filter paper method with prefreezing and keeping under light was used. Each test sample comprised 500 seeds. Pathogenicity of collected fungal isolates was tested following two laboratory methods. 238 seed samples were studied. 18 fungal species were found but only 7 proved to be important pathogens of root parsley. The most common inhabitants of root parsley seeds were Alternaria spp. A.allernata occurred on 74,8% of seeds but only a few isolates showed to be slightly pathogenic while A.petroselini and A.radicina were higly pathogenic and inhabited 11,4 and 4,2% of seeds, respectively. The second group of important pathogens were species of Fusarium found on 3,9% of seeds. F.avenaceum dominated as it comprised 48% of Fusarium isolates, the next were as follow: F.culmorum - 20%, F.equiseti - 15%, F.solani - 8%, F.oxysporum - 7% and F.dimerum -2%. Some fungi like Botrytis cinerea, Septoria petroselini and Phoma spp. inhabited low number of seeds, respectively O,4; 0,5 and 0,8%, but they were highly pathogenic to root parsley. The fungi: Bipolaris sorokiniana, Drechslera biseptata, Stemphylium botryosum and Ulocludium consortiale showed slight pathogenicity. They were isolated from 3,8% of seeds.

  19. Microscale Insight into Microbial Seed Banks.

    Science.gov (United States)

    Locey, Kenneth J; Fisk, Melany C; Lennon, J T

    2016-01-01

    Microbial dormancy leads to the emergence of seed banks in environmental, engineered, and host-associated ecosystems. These seed banks act as reservoirs of diversity that allow microbes to persist under adverse conditions, including extreme limitation of resources. While microbial seed banks may be influenced by macroscale factors, such as the supply of resources, the importance of microscale encounters between organisms and resource particles is often overlooked. We hypothesized that dimensions of spatial, trophic, and resource complexity determine rates of encounter, which in turn, drive the abundance, productivity, and size of seed banks. We tested this using >10,000 stochastic individual based models (IBMs) that simulated energetic, physiological, and ecological processes across combinations of resource, spatial, and trophic complexity. These IBMs allowed realistic dynamics and the emergence of seed banks from ecological selection on random variation in species traits. Macroscale factors like the supply and concentration of resources had little effect on resource encounter rates. In contrast, encounter rates were strongly influenced by interactions between dispersal mode and spatial structure, and also by the recalcitrance of resources. In turn, encounter rates drove abundance, productivity, and seed bank dynamics. Time series revealed that energetically costly traits can lead to large seed banks and that recalcitrant resources can lead to greater stability through the formation of seed banks and the slow consumption of resources. Our findings suggest that microbial seed banks emerge from microscale dimensions of ecological complexity and their influence on resource limitation and energetic costs.

  20. Inheritance of seed coat color in sesame

    Directory of Open Access Journals (Sweden)

    Hernán Laurentin

    2014-04-01

    Full Text Available The objective of this work was to determine the inheritance mode of seed coat color in sesame. Two crosses and their reciprocals were performed: UCLA37 x UCV3 and UCLA90 x UCV3, of which UCLA37 and UCLA90 are white seed, and UCV3 is brown seed. Results of reciprocal crosses within each cross were identical: F1 seeds had the same phenotype as the maternal parent, and F2 resulted in the phenotype brown color. These results are consistent only with the model in which the maternal effect is the responsible for this trait. This model was validated by recording the seed coat color of 100 F2 plants (F3 seeds from each cross with its reciprocal, in which the 3:1 expected ratio for plants producing brown and white seeds was tested with the chi-square test. Sesame seed color is determined by the maternal genotype. Proposed names for the alleles participating in sesame seed coat color are: Sc1, for brown color; and Sc2, for white color; Sc1 is dominant over Sc2.

  1. Evaluation of seed vigor tests for determinig alfalfa seed quality

    Directory of Open Access Journals (Sweden)

    hamidreza tavakoli kakhki

    2009-06-01

    Full Text Available In order to determine the best seed vigor test in laboratory which may provide high correlation between alfalfa seedling stablishment in the field, two experiment were conducted in field as well as in laboratory. Four alfalfa cultivars Bami, Ghareyonjeh, Hamadani and Yazdi were used. Different seed vigor tests including standard germination, germination in low tempretaure (5c , germination rate, osmotic stress, accelerated ageing and electrical conductivity tests were laid out in a randomized completely design (RCD with four replications in laboratory experiment‌. The second experiment was carried out in a randomized completely block design (RCBD and each treatment replicated four times‌. Results revealed significant differences (p≤ 0.01 among different cultivars for establishment percentage and rate. Results also showed that there were significant correlations between electrical conductivity test and establishment percentage (r= -0.65 , p ≤ 0.01 as well as establishment rate (r= -0.80 , p ≤ 0.01. Correlation coefficients for rate and emergence percentage were significant and positive (r= 0.91, p ≤ 0.01 . Stepwise method disclosed that the regression model (y=165.23 – 0.15 x r2=0.64 , (p ≤ 0.01 for predicting rate of emergence could be recommended. In this model x (dependent variable is value of electrical conductivity and y (independent variable is rate of emergence.

  2. Transcript profiles uncover temporal and stress-induced changes of metabolic pathways in germinating sugar beet seeds

    Directory of Open Access Journals (Sweden)

    Windhövel Andrea

    2008-12-01

    Full Text Available Abstract Background With a cultivation area of 1.75 Mio ha and sugar yield of 16.7 Mio tons in 2006, sugar beet is a crop of great economic importance in Europe. The productivity of sugar beet is determined significantly by seed vigour and field emergence potential; however, little is known about the molecular mechanisms underlying these traits. Both traits exhibit large variations within sugar beet germplasm that have been difficult to ascribe to either environmental or genetic causes. Among potential targets for trait improvement, an enhancement of stress tolerance is considered because of the high negative influence of environmental stresses on trait parameters. Extending our knowledge of genetic and molecular determinants of sugar beet germination, stress response and adaptation mechanisms would facilitate the detection of new targets for breeding crop with an enhanced field emergence potential. Results To gain insight into the sugar beet germination we initiated an analysis of gene expression in a well emerging sugar beet hybrid showing high germination potential under various environmental conditions. A total of 2,784 ESTs representing 2,251 'unigenes' was generated from dry mature and germinating seeds. Analysis of the temporal expression of these genes during germination under non-stress conditions uncovered drastic transcriptional changes accompanying a shift from quiescent to metabolically active stages of the plant life cycle. Assay of germination under stressful conditions revealed 157 genes showing significantly different expression patterns in response to stress. As deduced from transcriptome data, stress adaptation mechanisms included an alteration in reserve mobilization pathways, an accumulation of the osmoprotectant glycine betaine, late embryogenesis abundant proteins and detoxification enzymes. The observed transcriptional changes are supposed to be regulated by ABA-dependent signal transduction pathway. Conclusion This study

  3. Chemical Characteristics of Pumpkin Seed Tempeh From Soybean and Pumpkin Seeds

    OpenAIRE

    Pujilestari, Shanti; Sandrasari, Diny A; Marida, Rimmaria

    2017-01-01

    The aim of this research was to find the effect of the combination of soybean and pumpkin seeds on the chemical characteristics of pumpkin seed tempeh. The pumpkin seed tempeh samples were analyzed for its water, ash, protein, fat, carbohydrate and crude fiber. Meanwhile, support data were isoflavone and zinc. Sensory hedonic was conducted by 25 untrained panelists for selecting the best formulation of soybean and pumpkin seeds in tempeh. The result shows the formulation of soybean and pumpki...

  4. Seed Placement in Permanent Breast Seed Implant Brachytherapy: Are Concerns Over Accuracy Valid?

    Energy Technology Data Exchange (ETDEWEB)

    Morton, Daniel, E-mail: dmorton@bccancer.bc.ca [Department of Medical Physics, BC Cancer Agency, Centre for the Southern Interior, Kelowna, British Columbia (Canada); Department of Physics and Astronomy, University of Victoria, Victoria, British Columbia (Canada); Hilts, Michelle [Department of Medical Physics, BC Cancer Agency, Centre for the Southern Interior, Kelowna, British Columbia (Canada); Department of Physics and Astronomy, University of Victoria, Victoria, British Columbia (Canada); Batchelar, Deidre [Department of Medical Physics, BC Cancer Agency, Centre for the Southern Interior, Kelowna, British Columbia (Canada); Crook, Juanita [Department of Radiation Oncology, BC Cancer Agency, Centre for the Southern Interior, Kelowna, British Columbia (Canada)

    2016-07-01

    Purpose: To evaluate seed placement accuracy in permanent breast seed implant brachytherapy (PBSI), to identify any systematic errors and evaluate their effect on dosimetry. Methods and Materials: Treatment plans and postimplant computed tomography scans for 20 PBSI patients were spatially registered and used to evaluate differences between planned and implanted seed positions, termed seed displacements. For each patient, the mean total and directional seed displacements were determined in both standard room coordinates and in needle coordinates relative to needle insertion angle. Seeds were labeled according to their proximity to the anatomy within the breast, to evaluate the influence of anatomic regions on seed placement. Dosimetry within an evaluative target volume (seroma + 5 mm), skin, breast, and ribs was evaluated to determine the impact of seed placement on the treatment. Results: The overall mean (±SD) difference between implanted and planned positions was 9 ± 5 mm for the aggregate seed population. No significant systematic directional displacements were observed for this whole population. However, for individual patients, systematic displacements were observed, implying that intrapatient offsets occur during the procedure. Mean displacements for seeds in the different anatomic areas were not found to be significantly different from the mean for the entire seed population. However, small directional trends were observed within the anatomy, potentially indicating some bias in the delivery. Despite observed differences between the planned and implanted seed positions, the median (range) V{sub 90} for the 20 patients was 97% (66%-100%), and acceptable dosimetry was achieved for critical structures. Conclusions: No significant trends or systematic errors were observed in the placement of seeds in PBSI, including seeds implanted directly into the seroma. Recorded seed displacements may be related to intrapatient setup adjustments. Despite observed seed

  5. Seed abnormalities and associated mycoflora of rain- fed wheat ...

    African Journals Online (AJOL)

    Owner

    seeds with discoloured embryo (germ) (1.2 – 1.5%) and brush (0.25 – 1.25%) ends. Fusarium graminearum and Helminthosporium sativum were associated with all seeds, ... Key words: Fungi, seed health testing, seed discolouration, wheat. INTRODUCTION. Abnormality in seeds is a major constraint in crop production in ...

  6. Nutritional value OF Bottle Gourd ( Lagenaria siceraria ) Seeds ...

    African Journals Online (AJOL)

    Whole seeds, dehulled seeds and seed coats of bottle gourd seed (Lagenaria siceraria) were analysed for their proximate, amino acids and mineral compositions. The results of the analysis showed that, whole seed has highest content of moisture (17.5 0.21%) and ash (5.80 0.83%) while dehulled had highest amount ...

  7. Use of linear discriminant function analysis in seed morphotype ...

    African Journals Online (AJOL)

    Variation in seed morphology of the Lima bean in 31 accessions was studied. Data were collected on 100-seed weight, seed length and seed width. The differences among the accessions were significant, based on the three seed characteristics. K-means cluster analysis grouped the 31 accessions into four distinct groups, ...

  8. Ecological correlates of seed survival after ingestion by Fallow Deer

    NARCIS (Netherlands)

    Mouissie, AM; Van der Veen, CEJ; Veen, GF; Van Diggelen, R

    1. The survival and retention of seeds was studied by feeding known quantities of seeds of 25 species to four captive Fallow Deer (Dama dama L.). To test for ecological correlates, plant species were selected to represent large variation in seed size, seed shape, seed longevity and habitat

  9. 7 CFR 201.57a - Dormant seeds.

    Science.gov (United States)

    2010-01-01

    ... REGULATIONS Germination Tests in the Administration of the Act § 201.57a Dormant seeds. Dormant seeds are viable seeds, other than hard seeds, which fail to germinate when provided the specified germination..., or application of germination promoting chemicals. (b) The percentage of dormant seed, if present...

  10. Seed regulation: choices on the road to reform

    NARCIS (Netherlands)

    Tripp, R.; Louwaars, N.P.

    1997-01-01

    Major changes in national seed systems, including the rapid development of commercial seed enterprises, the growth of non-governmental organization (NGO) seed projects, and the concomitant decline of public sector seed provision, call for a re-examination of seed regulatory frameworks in developing

  11. Conference Proceedings: Seed Ecology III - The Third International Society for Seed Science Meeting on Seeds and the Environment - "Seeds and Change"; June 20-June 24, 2010; Salt Lake City, Utah, USA

    Science.gov (United States)

    Rosemary Pendleton; Susan Meyer; Bitsy Schultz

    2010-01-01

    Seed Ecology III was held in Salt Lake City, Utah in June 2010, sharing the latest research on all aspects of seed ecology. Our meeting was organized around the theme "Seeds and Change." We welcomed contributions in any area of seed ecology. Our agenda also aimed to create bridges between seed ecology and plant conservation, restoration ecology, and global...

  12. Factors affecting the density of Brassica napus seeds

    NARCIS (Netherlands)

    Young, L.; Jalink, H.; Denkert, R.; Reaney, M.

    2006-01-01

    Brassica napus seed is composed of low density oil (0.92 g.cm(-3)) and higher density solids (1.3-1.45 g.cm(-3)). Seed buoyant density may potentially be used to determine seed oil content and to separate seeds with different oil contents, however, we have found that seeds with the lowest buoyant

  13. Lipids in citrus sinensis seeds

    International Nuclear Information System (INIS)

    Hamid, S.; Liaquat, L.; Khalid, B.; Khan, J.I.

    2003-01-01

    The seed oil of citrus sinensis when subjected to different physicochemical tests showed moisture 13.2%, ash 7.5%, ester value 1.29%, free fatty acid 0.4%. iodine value 65.0% and protein value 6.0%. According to lipid analysis. the oil was classified into hydrocarbons. wax esters, sterol esters, triglycerides. free fatty acids, 1,3 and 1,2 diglycerides, alcohols, sterols, monoglycerides, phosphatidylethanolamines, phosphatidylcholines and lysophosphatidylethanolamines. The fatty acid (C/sub 12.0/ - C/sub 21.0/) composition of all lipid classes was determined with the help of thin layer and gas liquid chromatography. (author)

  14. Evaluation of the physiological quality of anadenenthera peregrina (L. Speg. seeds during storage Avaliação da qualidade fisiológica de sementes de Anadenanthera peregrina (L. Speg. durante o armazenamento

    Directory of Open Access Journals (Sweden)

    Daniel Santos Pinho

    2009-02-01

    Full Text Available The aim of this experiment was to evaluate the effect of storage temperature on the viability and vigour of A. peregrina seeds. Seeds of A. peregrina harvested in Viçosa-MG, in September 2005 were used. The seeds were stored in the Laboratory of Forest Seeds Analysis (LASF of the Universidade Federal de Viçosa (UFV, in a cold chamber, under approximately 5ºC over150 days. The seeds were stored under 20ºC for 150 days more. Seed moisture was determined after oven-drying at 105 ± 3ºC for 24 hours. The seeds were analyzed monthly using germination, tetrazolium and electrical conductivity tests and Osmopriming during 300 days, with four repetitions of 25 seeds. A. peregrina seeds kept a constant percentage of germination and viable seeds under the tetrazolium test for five months of storage, under either 5ºC and 20ºC. The electrical conductivity test showed significant differences in relation to the storage period, proving to be more sensitive than the standard germination test. Seed priming in PEG -0,4 MPa solution was not efficient in recovering the viability and vigor of the stored seeds at 5ºC and 20ºC.Este trabalho teve como objetivo avaliar o efeito da temperatura de armazenamento na viabilidade e vigor de sementes de angico (A. peregrina. As sementes utilizadas neste estudo foram colhidas na região de Viçosa, MG, em setembro de 2005, e armazenadas em câmara fria no Laboratório de Análise de Sementes Florestais (LASF da Universidade Federal de Viçosa (UFV por 150 dias, em uma temperatura de aproximadamente 5 ºC. A partir daí, as sementes foram armazenadas a aproximadamente a 20 ºC por mais 150 dias. O teor de água das sementes foi determinado após a secagem em estufa a 105 ± 3 ºC por 24 h. As sementes foram analisadas, mensalmente, pelo teste de germinação, teste de tetrazólio, teste de condutividade elétrica e osmocondicionamento, durante os 300 dias de armazenamento e com quatro repetições de 25 sementes. As

  15. Seed size, shape and vertical distribution in the soil : indicators of seed longevity

    NARCIS (Netherlands)

    Bekker, RM; Bakker, JP; Grandin, U; Kalamees, R; Milberg, P; Poschlod, P; Thompson, K; Willems, JH

    1998-01-01

    1. We investigated the vertical distribution of seeds in the soil, using data from nine studies in five European countries. We discovered significant correlations between seed shape and distribution in the soil. 2. The classification of the longevity of seeds of plant species has been improved by

  16. Proanthocyanidins in seed coat tegmen and endospermic cap inhibit seed germination in Sapium sebiferum.

    Science.gov (United States)

    Shah, Faheem Afzal; Ni, Jun; Chen, Jing; Wang, Qiaojian; Liu, Wenbo; Chen, Xue; Tang, Caiguo; Fu, Songling; Wu, Lifang

    2018-01-01

    Sapium sebiferum , an ornamental and bio-energetic plant, is propagated by seed. Its seed coat contains germination inhibitors and takes a long time to stratify for germination. In this study, we discovered that the S. sebiferum seed coat (especially the tegmen) and endospermic cap (ESC) contained high levels of proanthocyanidins (PAs). Seed coat and ESC removal induced seed germination, whereas exogenous application with seed coat extract (SCE) or PAs significantly inhibited this process, suggesting that PAs in the seed coat played a major role in regulating seed germination in S. sebiferum . We further investigated how SCE affected the expression of the seed-germination-related genes. The results showed that treatment with SCE upregulated the transcription level of the dormancy-related gene, gibberellins (GAs) suppressing genes, abscisic acid (ABA) biosynthesis and signalling genes. SCE decreased the transcript levels of ABA catabolic genes, GAs biosynthesis genes, reactive oxygen species genes and nitrates-signalling genes. Exogenous application of nordihydroguaiaretic acid, gibberellic acid, hydrogen peroxide and potassium nitrate recovered seed germination in seed-coat-extract supplemented medium. In this study, we highlighted the role of PAs, and their interactions with the other germination regulators, in the regulation of seed dormancy in S. sebiferum .

  17. Supplementing seed banks to rehabilitate disturbed Mojave Desert shrublands: where do all the seeds go?

    Science.gov (United States)

    DeFalco, Lesley A.; Esque, Todd C.; Nicklas, Melissa B.; Kane, Jeffrey M.

    2012-01-01

    Revegetation of degraded arid lands often involves supplementing impoverished seed banks and improving the seedbed, yet these approaches frequently fail. To understand these failures, we tracked the fates of seeds for six shrub species that were broadcast across two contrasting surface disturbances common to the Mojave Desert—sites compacted by concentrated vehicle use and trenched sites where topsoil and subsurface soils were mixed. We evaluated seedbed treatments that enhance soil-seed contact (tackifier) and create surface roughness while reducing soil bulk density (harrowing). We also explored whether seed harvesting by granivores and seedling suppression by non-native annuals influence the success of broadcast seeding in revegetating degraded shrublands. Ten weeks after treatments, seeds readily moved off of experimental plots in untreated compacted sites, but seed movements were reduced 32% by tackifier and 55% through harrowing. Harrowing promoted seedling emergence in compacted sites, particularly for the early-colonizing species Encelia farinosa, but tackifier was largely ineffective. The inherent surface roughness of trenched sites retained three times the number of seeds than compacted sites, but soil mixing during trench development likely altered the suitability of the seedbed thus resulting in poor seedling emergence. Non-native annuals had little influence on seed fates during our study. In contrast, the prevalence of harvester ants increased seed removal on compacted sites, whereas rodent activity influenced removal on trenched sites. Future success of broadcast seeding in arid lands depends on evaluating disturbance characteristics prior to seeding and selecting appropriate species and seasons for application.

  18. Evaluation of non-chemical seed treatment methods for control of Alternaria brassicicola on cabbage seeds

    NARCIS (Netherlands)

    Amein, T.; Wright, Al S.; Wikstrom, M.; Koch, E.; Schmitt, A.; Stephan, D.; Jahn, M.; Tinivella, F.; Gullino, M.L.; Forsberg, G.; Werner, S.; Wolf, van der J.M.; Groot, S.P.C.

    2011-01-01

    Due to the lack of foliar fungicide use, the organic production of Brassica seeds free of Alternaria spp. is difficult. Therefore, effective seed treatments certified for use in organic farming are needed to eradicate or at least effec­tively reduce the seed-borne inoculum. We here report results of

  19. Bulk YBCO seeded with 45°–45° bridge-seeds of different lengths

    International Nuclear Information System (INIS)

    Shi, Y-H; Durrell, J H; Dennis, A R; Cardwell, D A

    2013-01-01

    Single grain, (RE)BCO (rare earth–barium–copper oxide) bulk superconductors in large or complicated geometries are required for a variety of potential applications, such as in motors and generators and magnetic shielding devices. As a result, top, multi-seeded, melt growth has been investigated over the past 15 years in an attempt to enlarge the size of (RE)BCO single grains specifically for such applications. Of these multi-seeding techniques, so-called bridge-seeding provides the best alignment of two seeds in a single grain growth process. Here we report, for the first time, the successful growth of YBCO (yttrium–barium–copper oxide) using a special, 45°–45°, arrangement of bridge-seeds. The superconducting properties, including trapped field, of the multi-seeded YBCO grains have been measured for different bridge lengths of the 45°–45° bridge-seeds. The boundaries at the impinging growth front and the growth features of the top, multi-seeded surface and cross-section of the multi-seeded, samples have been analysed using optical microscopy. The results suggest that an impurity-free boundary between the two seeds of each leg of the bridge-seed can form when 45°–45° bridge-seeds are used to enlarge the size of YBCO grains. (paper)

  20. Estimating Cone and Seed Production and Monitoring Pest Damage in Southern Pine Seed Orchards

    Science.gov (United States)

    Carl W. Fatzinger; H. David Muse; Thomas Miller; Helen T. Bhattacharyya

    1988-01-01

    Field sampling procedures and computer programs are described for monitoring seed production and pest damage in southern pine seed orchards. The system estimates total orchard yields of female strobili and seeds, quantifies pest damage, determines times of year when losses occur, and produces life tables for female strobili. An example is included to illustrate the...