WorldWideScience

Sample records for sealed uv sensitive

  1. Sensitivity analysis overlaps of friction elements in cartridge seals

    Directory of Open Access Journals (Sweden)

    Žmindák Milan

    2018-01-01

    Full Text Available Cartridge seals are self-contained units consisting of a shaft sleeve, seals, and gland plate. The applications of mechanical seals are numerous. The most common example of application is in bearing production for automobile industry. This paper deals with the sensitivity analysis of overlaps friction elements in cartridge seal and their influence on the friction torque sealing and compressive force. Furthermore, it describes materials for the manufacture of sealings, approaches usually used to solution of hyperelastic materials by FEM and short introduction into the topic wheel bearings. The practical part contains one of the approach for measurement friction torque, which results were used to specifying the methodology and precision of FEM calculation realized by software ANSYS WORKBENCH. This part also contains the sensitivity analysis of overlaps friction elements.

  2. UV sensitivity of various solid state detectors

    International Nuclear Information System (INIS)

    Knezevic, Zeljka; Ranogajec-Komor, Maria; Miljanic, Saveta

    2008-01-01

    Full text: The light sensitivity is an important characteristic of solid state passive dosimeters used in individual, clinical and environmental dosimetry. Light sensitivity stands for the response directly induced by visible or UV light in a fully annealed material. For the above mentioned applications a negligible light sensitivity is an advantage. However, high light sensitivity and linear response allows the use of detectors as UV dosimeters. For this purpose various TL detectors and the glass element of the RPL dosemeter type SC-1 were systematically investigated after exposure to UV light (254 and 366 nm) as a function of time. The following solid state detectors were investigated relative to TLD-100: Li 2 B 4 O 7 :Cu,Ag,P LiF:Mg,Cu,P, LiF:Mg,Cu,Si, Al 2 O 3 :C and the glass element of RPL dosimeter. UV irradiations were performed with Camag UV lamp at 254 nm and at 366 nm. The illumination times were 5, 10 and 20 minutes. Day light illumination was also carried out at room temperature over time period of several hours up to 2 weeks. The UV light response of each detector was compared to the response obtained after irradiation with 137 Cs. Al 2 O 3 :C, showed high light sensitivity; after 10 minutes illumination with 254 nm UV light the response was equivalent to 130 mGy 137 Cs gamma irradiation. The 254 nm UV response of LiF:Mg,Cu,P (GR-200 A), as well as TLD-700H and Li 2 B 4 O 7 :Cu,Ag,P were proportional to the time of illumination. The responses after 10 min UV illumination were equivalent to 0.001 mGy, 0.01 mGy and 0.1 mGy 137 Cs gamma irradiation, respectively. The complete SC-1 RPL dosimeter is insensitive to light because the glass element is encapsulated in light protected holder throughout the automatic evaluation process following the annealing (irradiation, preheat, readout). The responses of the previously annealed glass element after 20 min illumination with 254 nm and 366 nm UV light were equivalent to 45μSv and 3 μSv of 137 Cs gamma

  3. Sealed two-electrode photoelectrochemical cell based on nanocrystalline TiO2 analyzed as a UV sensor

    International Nuclear Information System (INIS)

    Forcade, Fresnel; Gonzalez, Bernardo; Maqueda, Ma. de la Luz; Curbelo, Larisa; Vigil, Elena; Jennings, James R.; Dunn, Halina; Wang, Hongxia; PeteR, Lauri M.

    2008-01-01

    Potentialities as UV sensor of a sealed two-electrode photoelectrochemical cell (PEC) based on nanocrystalline TiO 2 are analyzed. Ultraviolet component of solar light is responsible for a number of skin disorders and diseases. An inexpensive and simple UV sensor would be convenient to measure the UV intensity been exposed to. Nanocrystalline TiO 2 is a rather inexpensive material, innocuous and very stable which is intensively studied at the present moment because of its possible applications in dye-sensitized solar cells, photocatalysis, electrochromics, etc. The method for obtaining the sensor and its structure are described. Different TiO 2 layer structures for the photoelectrode are studied. Important parameters, such as, spectral response, external quantum efficiency, current vs light intensity and current-voltage curve are presented. Results show prospective for the implementation of this type of sensor. (Full text)

  4. Expression of UV-irradiated adenovirus in normal and UV-sensitive Chinese hamster ovary cells

    International Nuclear Information System (INIS)

    Rainbow, A.J.

    1985-01-01

    The chinese hamster ovary (CHO) cell mutants UV-20, UV-24, and UV-41 are abnormally sensitive to UV and harbour various defects lin their ability to repair cellular DNA. This study has examined the expression of UV-irradiated AD2 in these cells. HCR of UV-irradiated Ad2, as measured by viral structural antigen (Vag) formation or progeny production, was found to be similar for the normal and the UV-sensitive CHO strains. UV-irradiation of Ad2 (1200 J/m/sup 2/) resulted in a delay of Vag expression of 18 hours in normal human fibroblasts, which is thought to reflect the time required for removal of UV-induced lesions from the DNA before viral DNA synthesis can proceed. However, a similar UV-irradiation of Ad2 did not result in a delay of Vag expression for infection of CHO cells, suggesting that UV-induced lesions in Ad2 DNA do not inhibit its replication in CHO cells. These results indicate a fundamental difference in the processing of UV-irradiated AD2-DNA in CHO as compared to human cells

  5. Improving radiochromic film's sensitivity by wrapping it with UV phosphor

    International Nuclear Information System (INIS)

    Geso, Moshi; Ackerly, Trevor; Patterson, William

    2004-01-01

    The main advantage of radiochromic-film dosimeters is the coupling of rapid full planar-acquisition, high-spatial resolution and dose linearity. Their main limitation, however, is their low radiation sensitivity. This precludes their application to measuring doses below a few Gy. Radiochromic films are sensitive to ultraviolet radiation. In this note the results of exposing radiochromic films to x rays through an UV imaging cassette, which converts and amplifies x rays to UV radiation, are presented. These results indicate a clear increase (around 50%) in radiochromic film's sensitivity to MV x rays (6, 10, and 18 MV) when exposed through the UV phosphor

  6. Thermoluminescence sensitivity of ulexite after UV irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Topaksu, M., E-mail: mats@cu.edu.tr [Cukurova University, Arts-Sciences Faculty, Physics Department, 01330 Adana (Turkey); CIEMAT, Av. Complutense 22, 28040 Madrid (Spain); Correcher, V. [CIEMAT, Av. Complutense 22, 28040 Madrid (Spain); Garcia-Guinea, J. [Museo Nacional de Ciencias Naturales, (CSIC), C/José Gutiérrez Abascal 2, 28006 Madrid (Spain)

    2015-04-15

    Highlights: •UV-induced thermoluminescence emission of Turkish ulexite was studied. •There are three groups of components. •The UV exposures were performed at controlled temperatures. -- Abstract: The effects of UV radiation on the thermoluminescence (TL) properties of natural materials, in contrast to synthetic materials, have been scarcely studied. We report on the UV-induced thermoluminescence emission of a Turkish ulexite (NaCaB{sub 5}O{sub 6}(OH){sub 6}·5H{sub 2}O) that displays very complex TL glow curves, with at least three groups of components peaked at 130–140 °C, 240 °C and, 340 °C, wherein the last group is weaker. Such emission could be associated with structural changes in the lattice as well as alkali self-diffusion processes. The UV exposure performed at controlled temperatures (at room temperature (RT), 50 °C and 100 °C) produced a (i) different evolutions of the intensities of each maximum, which are directly related to the controlled thermal treatment; (ii) different intensity ratios among the groups of components; (iii) different activation energies (E{sub a}) (1.13 eV for RT, 0.99 eV for 50 °C and 0.49 eV for 100 °C) calculated using the initial rise method; and (iv) similar scattering values (12.4%, 8.2% and 12.8%), which were not a function of the controlled temperature. The thermal stability tests conducted on this borate at different temperatures, based on the T{sub stop} protocol, confirm the presence of a continuum in the distribution of the trap system with progressively increasing E{sub a} (from 0.60 to 0.90 eV)

  7. Bovine ocular squamous cell carcinoma: UV sensitivity in lymphocytes

    Energy Technology Data Exchange (ETDEWEB)

    Lavin, M.F.; Jennings, P.A.; Hughes, D.J. (Queensland Univ., Brisbane (Australia))

    1982-05-01

    Increased sensitivity to UV light has been demonstrated in Phytohaemagglutinin stimulated lymphocytes from normal and tumour-bearing Hereford cattle when compared to lymphocytes from other breeds. Trypan blue exclusion and inhibition of DNA synthesis were used to determine cell viability. The results obtained from time course and radiation dose experiments demonstrate biphasic survival kinetics. This is indicative of at least two separate cell populations, exhibiting differential sensitivity to UV. The increased sensitivity to UV observed in Herefords may reflect a general sensitivity to UV or alternatively a different cellular constitution in the mitogen stimulated cultures. DNA repair synthesis, measured in the presence of hydroxyurea, was of similar levels in cell cultures from Herefords and one of the control breeds.

  8. Bovine ocular squamous cell carcinoma: UV sensitivity in lymphocytes

    International Nuclear Information System (INIS)

    Lavin, M.F.; Jennings, P.A.; Hughes, D.J.

    1982-01-01

    Increased sensitivity to UV light has been demonstrated in Phytohaemagglutinin stimulated lymphocytes from normal and tumour-bearing Hereford cattle when compared to lymphocytes from other breeds. Trypan blue exclusion and inhibition of DNA synthesis were used to determine cell viability. The results obtained from time course and radiation dose experiments demonstrate biphasic survival kinetics. This is indicative of at least two separate cell populations, exhibiting differential sensitivity to UV. The increased sensitivity to UV observed in Herefords may reflect a general sensitivity to UV or alternatively a different cellular constitution in the mitogen stimulated cultures. DNA repair synthesis, measured in the presence of hydroxyurea, was of similar levels in cell cultures from Herefords and one of the control breeds. (author)

  9. UV-sensitivity and repair of UV-damage in Salmonella of wild type

    International Nuclear Information System (INIS)

    Kondratiev, Y.S.; Brukhansky, G.V.; Andreeva, I.V.; Skavronskaya, A.G.

    1977-01-01

    The UV-sensitivity of wild type Salmonella strains has been compared to that of wild type E.coli and its UV-sensitive mutants. Many wild type Salmonella strains are 4-5 times more sensitive than wild type E.coli and their inactivation curve is similar to that for E.coli with a mutation in the polA gene. Alkaline sucrose gradient centrifugation has shown a deficiency of these strains in normal excision repair of UV-damaged DNA. This deficiency is not a Salmonella genus feature because one strain as resistant as wild type E.coli was found. This resistant strain showed normal excision repair in alkaline sucrose gradient centrifugation experiments. The possible influence of plasmids and mutations in repair genes on the ability of Salmonella to repair UV-damaged DNA is discussed. (orig.) [de

  10. UV-sensitivity and repair of UV-damage in Salmonella of wild type

    Energy Technology Data Exchange (ETDEWEB)

    Kondratiev, Y S; Brukhansky, G V; Andreeva, I V; Skavronskaya, A G [Akademiya Meditsinskikh Nauk SSSR, Moscow. Inst. Ehpidemiologii i Mikrobiologii

    1977-12-01

    The UV-sensitivity of wild type Salmonella strains has been compared to that of wild type E.coli and its UV-sensitive mutants. Many wild type Salmonella strains are 4-5 times more sensitive than wild type E.coli and their inactivation curve is similar to that for E.coli with a mutation in the polA gene. Alkaline sucrose gradient centrifugation has shown a deficiency of these strains in normal excision repair of UV-damaged DNA. This deficiency is not a Salmonella genus feature because one strain as resistant as wild type E.coli was found. This resistant strain showed normal excision repair in alkaline sucrose gradient centrifugation experiments. The possible influence of plasmids and mutations in repair genes on the ability of Salmonella to repair UV-damaged DNA is discussed.

  11. Seals

    International Nuclear Information System (INIS)

    Welsher, R.A.G.

    1982-01-01

    An aperture through a biological shield is sealed by a flexible sheath having a beading at one end located on an annular member slidable in the aperture such that the beading bears in sealing engagement against the sides of the aperture. The annular member is retained by a retractable latch and can be rejected by pushing it out of the aperture using a replacement annular member with a replacement sheath thereon to butt against the annular member to be rejected. The replacement annular member may be mounted on a tubular device having an outer co-axial member for operating the latch when the replacement annular member butts against the annular member to be rejected. Applications include effecting a seal between a remote handling equipment and a wall through which the equipment extends. (author)

  12. Fabrics Protect Sensitive Skin from UV Rays

    Science.gov (United States)

    2009-01-01

    Late Johnson Space Center engineer Dr. Robert Dotts headed a team to develop cool suits for children suffering from life-threatening sun sensitivities. Dotts hoped to develop ultraviolet-blocking technology in a fabric that -- unlike in a bulky space suit -- could remain comfortable, light, and breathable in the sun and heat. The team worked with SPF 4 US LLC (SPF) of Madison, Wisconsin to design ultraviolet-blocking cool suits, which protect sun-sensitive patients and enable them to experience life outdoors safely. Using knowledge gained during the NASA collaboration, SPF created an entire line of ultraviolet-blocking apparel.

  13. Plasmid-associated sensitivity of Bacillus thuringiensis to UV light

    International Nuclear Information System (INIS)

    Benoit, T.G.; Wilson, G.R.; Bull, D.L.; Aronson, A.I.

    1990-01-01

    Spores and vegetative cells of Bacillus thuringiensis were more sensitive to UV light than were spores or cells of plasmid-cured B. thuringiensis strains or of the closely related Bacillus cereus. Introduction of B. thuringiensis plasmids into B. cereus by cell mating increased the UV sensitivity of the cells and spores. Protoxins encoded by one or more B. thuringiensis plasmids were not involved in spore sensitivity, since a B. thuringiensis strain conditional for protoxin accumulation was equally sensitive at the permissive and nonpermissive temperatures. In addition, introduction of either a cloned protoxin gene, the cloning vector, or another plasmid not containing a protoxin gene into a plasmid-cured strain of B. thuringiensis all increased the UV sensitivity of the spores. Although the variety of small, acid-soluble proteins was the same in the spores of all strains examined, the quantity of dipicolinic acid was about twice as high in the plasmid-containing strains, and this may account for the differences in UV sensitivity of the spores. The cells of some strains harboring only B. thuringiensis plasmids were much more sensitive than cells of any of the other strains, and the differences were much greater than observed with spores

  14. A UV sensitive integrated micromegas with timepix readout

    NARCIS (Netherlands)

    Melai, J.; Breskin, Amos; Cortesi, Marco; Bilevych, Y.; Bilevych, Yevgen; Fransen, Martin; van der Graaf, Harry; Visschers, Jan; Blanco Carballo, V.M.; Salm, Cora; Schmitz, Jurriaan

    2010-01-01

    This article presents a detector system consisting of three components, a CMOS imaging array, a gaseous-detector structure with a Micromegas layout, and a UV-photon sensitive CsI reflective photocathode. All three elements have been monolithically integrated using simple post-processing steps. The

  15. UV- and gamma-radiation sensitive mutants of Arabidopsis thaliana

    International Nuclear Information System (INIS)

    Jiang, C.Z.; Yen, C.N.; Cronin, K.; Mitchell, D.; Britt, A.B.

    1997-01-01

    Arabidopsis seedlings repair UV-induced DNA damage via light-dependent and -independent pathways. The mechanism of the ''dark repair'' pathway is still unknown. To determine the number of genes required for dark repair and to investigate the substrate-specificity of this process we isolated mutants with enhanced sensitivity to UV radiation in the absence of photoreactivating light. Seven independently derived UV sensitive mutants were isolated from an EMS-mutagenized population. These fell into six complementation groups, two of which (UVR1 and UVH1) have previously been defined. Four of these mutants are defective in the dark repair of UV-induced pyrimidine [6-4] pyrimidinone dimers. These four mutant lines are sensitive to the growth-inhibitory effects of gamma radiation, suggesting that this repair pathway is also involved in the repair of some type of gamma-induced DNA damage product. The requirement for the coordinate action of several different gene products for effective repair of pyrimidine dimers, as well as the nonspecific nature of the repair activity, is consistent with nucleotide excision repair mechanisms previously described in Saccharomyces cerevisiae and nonplant higher eukaryotes and inconsistent with substrate-specific base excision repair mechanisms found in some bacteria, bacteriophage, and fungi. (author)

  16. Ultraviolet /UV/ sensitive phosphors for silicon imaging detectors

    Science.gov (United States)

    Viehmann, W.; Cowens, M. W.; Butner, C. L.

    1981-01-01

    The fluorescence properties of UV sensitive organic phosphors and the radiometric properties of phosphor coated silicon detectors in the VUV, UV, and visible wavelengths are described. With evaporated films of coronene and liumogen, effective quantum efficiencies of up to 20% have been achieved on silicon photodiodes in the vacuum UV. With thin films of methylmethacrylate (acrylic), which are doped with organic laser dyes and deposited from solution, detector quantum efficiencies of the order of 15% for wavelengths of 120-165 nm and of 40% for wavelengths above 190 nm have been obtained. The phosphor coatings also act as antireflection coatings and thereby enhance the response of coated devices throughout the visible and near IR.

  17. Damage to UV-sensitive cells by short UV in photographic flashes

    International Nuclear Information System (INIS)

    Menezes, S.; Monteiro, C.

    1996-01-01

    Light emitted by electronic photographic flash units is shown to damage bacteria and human skin fibroblasts deficient in repair systems, with survival curves very similar to those produced by 254 nm short UV. The lesions induced by these flashes are as photorepairable by the photolyase enzyme as those induced by 254 nm UV and result in equivalent survival rates. Biological dosimetry performed with microorganisms highly sensitive to UV (Escherichia coli K12 AB2480, deficient in excision and recombinational-dependent repair systems and Bacillus subtilis UVSSP spores, deficient in excision and in a specific spore repair process) revealed that each 1 ms flash of light from the photographic unit used in this work contained the equivalent of 0.25 J m -2 of 254 nm UV, when measured at a distance of 7.0 cm. This dose of UV was found to be lethal to both repair-deficient E. coli bacteria and repair-deficient human skin fibroblasts obtained from xeroderma pigmentosum donors, as well as mutagenic in B/r wild-type and HCR-mutant bacteria. (Author)

  18. Experiment of solidifying photo sensitive polymer by using UV LED

    Science.gov (United States)

    Kang, Byoung Hun; Shin, Sung Yeol

    2008-11-01

    The development of Nano/Micro manufacturing technologies is growing rapidly and in the same manner, the investments in these areas are increasing. The applications of Nano/Micro technologies are spreading out to semiconductor production technology, biotechnology, environmental engineering, chemical engineering and aerospace. Especially, SLA is one of the most popular applications which is to manufacture 3D shaped microstructure by using UV laser and photo sensitive polymer. To make a high accuracy and precision shape of microstructures that are required from the diverse industrial fields, the information of interaction relationship between the photo resin and the light source is necessary for further research. Experiment of solidifying photo sensitive polymer by using UV LED is the topic of this paper and the purpose of this study is to find out what relationships do the reaction of the resin have in various wavelength, power of the light and time.

  19. Presence of UV-endonuclease sensitive sites in daughter DNA of UV-irradiated mammalian cells

    International Nuclear Information System (INIS)

    D'Ambrosio, S.; Setlow, R.B.

    1978-02-01

    Asynchronous Chinese hamster cells were irradiated with 10 Jm -2 uv radiation and 0.25 to 4 hours later pulse-labeled with [ 3 H]thymidine. Cells synchronized by shaking off mitotic and G 1 cells were irradiated in either the G 1 -phase or S-phase of the cell cycle and pulse-labeled with [ 3 H]thymidine in the S-phase. After a 12 to 14 hour chase in unlabeled medium, the DNA was extracted, incubated with Micrococcus luteus uv-endonuclease and sedimented in alkaline sucrose. The number of endonuclease sensitive sites decreased as the time between uv irradiation and pulse-labeling of daughter DNA increased. Further, there were significantly less endonuclease sensitive sites in the daughter DNA from cells irradiated in the G 1 -phase than in the S-phase. These data indicate that very few, if any, dimers are transferred from parental DNA to daughter DNA and that the dimers detected in daughter DNA may be due to the irradiation of replicating daughter DNA before labeling

  20. Sensitivity Analysis on LOCCW of Westinghouse typed Reactors Considering WOG2000 RCP Seal Leakage Model

    International Nuclear Information System (INIS)

    Na, Jang-Hwan; Jeon, Ho-Jun; Hwang, Seok-Won

    2015-01-01

    In this paper, we focus on risk insights of Westinghouse typed reactors. We identified that Reactor Coolant Pump (RCP) seal integrity is the most important contributor to Core Damage Frequency (CDF). As we reflected the latest technical report; WCAP-15603(Rev. 1-A), 'WOG2000 RCP Seal Leakage Model for Westinghouse PWRs' instead of the old version, RCP seal integrity became more important to Westinghouse typed reactors. After Fukushima accidents, Korea Hydro and Nuclear Power (KHNP) decided to develop Low Power and Shutdown (LPSD) Probabilistic Safety Assessment (PSA) models and upgrade full power PSA models of all operating Nuclear Power Plants (NPPs). As for upgrading full power PSA models, we have tried to standardize the methodology of CCF (Common Cause Failure) and HRA (Human Reliability Analysis), which are the most influential factors to risk measures of NPPs. Also, we have reviewed and reflected the latest operating experiences, reliability data sources and technical methods to improve the quality of PSA models. KHNP has operating various types of reactors; Optimized Pressurized Reactor (OPR) 1000, CANDU, Framatome and Westinghouse. So, one of the most challengeable missions is to keep the balance of risk contributors of all types of reactors. This paper presents the method of new RCP seal leakage model and the sensitivity analysis results from applying the detailed method to PSA models of Westinghouse typed reference reactors. To perform the sensitivity analysis on LOCCW of the reference Westinghouse typed reactors, we reviewed WOG2000 RCP seal leakage model and developed the detailed event tree of LOCCW considering all scenarios of RCP seal failures. Also, we performed HRA based on the T/H analysis by using the leakage rates for each scenario. We could recognize that HRA was the sensitive contributor to CDF, and the RCP seal failure scenario of 182gpm leakage rate was estimated as the most important scenario

  1. Sensitivity Analysis on LOCCW of Westinghouse typed Reactors Considering WOG2000 RCP Seal Leakage Model

    Energy Technology Data Exchange (ETDEWEB)

    Na, Jang-Hwan; Jeon, Ho-Jun; Hwang, Seok-Won [KHNP Central Research Institute, Daejeon (Korea, Republic of)

    2015-10-15

    In this paper, we focus on risk insights of Westinghouse typed reactors. We identified that Reactor Coolant Pump (RCP) seal integrity is the most important contributor to Core Damage Frequency (CDF). As we reflected the latest technical report; WCAP-15603(Rev. 1-A), 'WOG2000 RCP Seal Leakage Model for Westinghouse PWRs' instead of the old version, RCP seal integrity became more important to Westinghouse typed reactors. After Fukushima accidents, Korea Hydro and Nuclear Power (KHNP) decided to develop Low Power and Shutdown (LPSD) Probabilistic Safety Assessment (PSA) models and upgrade full power PSA models of all operating Nuclear Power Plants (NPPs). As for upgrading full power PSA models, we have tried to standardize the methodology of CCF (Common Cause Failure) and HRA (Human Reliability Analysis), which are the most influential factors to risk measures of NPPs. Also, we have reviewed and reflected the latest operating experiences, reliability data sources and technical methods to improve the quality of PSA models. KHNP has operating various types of reactors; Optimized Pressurized Reactor (OPR) 1000, CANDU, Framatome and Westinghouse. So, one of the most challengeable missions is to keep the balance of risk contributors of all types of reactors. This paper presents the method of new RCP seal leakage model and the sensitivity analysis results from applying the detailed method to PSA models of Westinghouse typed reference reactors. To perform the sensitivity analysis on LOCCW of the reference Westinghouse typed reactors, we reviewed WOG2000 RCP seal leakage model and developed the detailed event tree of LOCCW considering all scenarios of RCP seal failures. Also, we performed HRA based on the T/H analysis by using the leakage rates for each scenario. We could recognize that HRA was the sensitive contributor to CDF, and the RCP seal failure scenario of 182gpm leakage rate was estimated as the most important scenario.

  2. The enhanced UV-sensitivity of Escherichia coli uvr A crp strain

    International Nuclear Information System (INIS)

    Skavronskaya, A.G.; Aleshkin, G.I.

    1979-01-01

    Mutations in genes cya and crp do not affect the UV cell sensitivity of Escherichia coli of wild type in relation to repairs of UV-injuries and UV induced mutations yield. Mutations in gene crp (protein defect of catabolitic activator - cap) result in UV sensitivity decrease of E. coli uvrA strain, imperfect as to the first stage of excision repairs not decreasing the quantity of revertants, induced by the UV-light

  3. Ringed Seal Search for Global Optimization via a Sensitive Search Model.

    Directory of Open Access Journals (Sweden)

    Younes Saadi

    Full Text Available The efficiency of a metaheuristic algorithm for global optimization is based on its ability to search and find the global optimum. However, a good search often requires to be balanced between exploration and exploitation of the search space. In this paper, a new metaheuristic algorithm called Ringed Seal Search (RSS is introduced. It is inspired by the natural behavior of the seal pup. This algorithm mimics the seal pup movement behavior and its ability to search and choose the best lair to escape predators. The scenario starts once the seal mother gives birth to a new pup in a birthing lair that is constructed for this purpose. The seal pup strategy consists of searching and selecting the best lair by performing a random walk to find a new lair. Affected by the sensitive nature of seals against external noise emitted by predators, the random walk of the seal pup takes two different search states, normal state and urgent state. In the normal state, the pup performs an intensive search between closely adjacent lairs; this movement is modeled via a Brownian walk. In an urgent state, the pup leaves the proximity area and performs an extensive search to find a new lair from sparse targets; this movement is modeled via a Levy walk. The switch between these two states is realized by the random noise emitted by predators. The algorithm keeps switching between normal and urgent states until the global optimum is reached. Tests and validations were performed using fifteen benchmark test functions to compare the performance of RSS with other baseline algorithms. The results show that RSS is more efficient than Genetic Algorithm, Particles Swarm Optimization and Cuckoo Search in terms of convergence rate to the global optimum. The RSS shows an improvement in terms of balance between exploration (extensive and exploitation (intensive of the search space. The RSS can efficiently mimic seal pups behavior to find best lair and provide a new algorithm to be

  4. Isolation of uv-sensitive variants of human FL cells by a viral suicide method

    International Nuclear Information System (INIS)

    Shiomi, T.; Sato, K.

    1979-01-01

    A new method (viral suicide method) for the isolation of uv-sensitive mutants is described. Colonies of mutagenized human FL cells were infected with uv-irradiated Herpes simplex viruses and surviving ones which seemed to be deficient in host cell reactivation (HCR) were examined for their uv sensitivity. Nineteen of 238 clones examined were sensitive to uv irradiation at the time of the isolation. After recloning, four of these clones have been studied and two (UVS-1 and UVS-2) of them are stable in their uv sensitivity for 4 months in culture. uv sensitivity of UVS-1, UVS-2, and the parental FL cells are as follows: the extrapolation numbers (n) are 2.2, 2.1, and 1.8 and mean lethal doses (DO) are 2.9, 3.7, and 7.8 J/m 2 for UVS-1, UVS-2, and the parental FL cells, respectively. They are no more sensitive than FL cells to x-irradiation. The ability of HCR in UVS-2 cells is apparently lower than that in FL cells, whereas UVS-1 cells are the same as FL cells in the ability

  5. Quantum bit commitment with cheat sensitive binding and approximate sealing

    Science.gov (United States)

    Li, Yan-Bing; Xu, Sheng-Wei; Huang, Wei; Wan, Zong-Jie

    2015-04-01

    This paper proposes a cheat-sensitive quantum bit commitment scheme based on single photons, in which Alice commits a bit to Bob. Here, Bob’s probability of success at cheating as obtains the committed bit before the opening phase becomes close to \\frac{1}{2} (just like performing a guess) as the number of single photons used is increased. And if Alice alters her committed bit after the commitment phase, her cheating will be detected with a probability that becomes close to 1 as the number of single photons used is increased. The scheme is easy to realize with present day technology.

  6. Uv Laser Excitation for Ultra-Sensitive Photoluminescent Dosimetry

    Energy Technology Data Exchange (ETDEWEB)

    Kastner, J.; Eggenberger, D.; Longnecker, A. [Argonne National Laboratory, Argonne, IL (United States); King, D.; Schutt, D. [Radiation Laboratory, University of Notre Dame, South Bend, IN (United States)

    1967-03-15

    The factor which has limited the sensitivity of photoluminescent dosimetry has been the ''pre-dose'' background which is stimulated during readout by the usual continuous ultra-violet (UV) exposure. The signal-to-noise ratio has only been partially optimized by the selective choice of filters and optical geometry. A microdosimetric system has been conceived and investigated which is potentially capable of sensing extremely low radiation doses (of the order of microrads). This system depends on the little-known fact that the decay time for the visible luminescence, which is a measure of the absorbed dose, is at least ten times longer than the decay of the indistinguishable visible fluorescence (to UV) which is an inherent characteristic of unexposed silver phosphate glasses. The system consists of UV, 3500A, laser beam, with a Pockels cell so that it has complete cut-off in intensity in the order of nanoseconds, and gating circuitry to open the visible light-sensing photomultiplier at a sufficient time delay to prevent it from sensing the ultra-violet or the pre-dose fluorescence which decays within the order of 100 nanoseconds. In this way the signal-to-noise ratio can be vastly improved upon that obtainable by optical means. With this system the authors were easily able to measure quantitatively one milliroentgen of cobalt-60 exposure. They are of the opinion that further improvement in this system should enable them to do track visualization and/or in vivo biological microdosimetry with a spatial resolution of the order of ten microns. (author)

  7. Different action of MMS and EMS in UV-sensitive strains of Aspergillus nidulans.

    Science.gov (United States)

    Babudri, N; Politi, M G

    1989-05-01

    The repair of methyl methanesulfonate (MMS) and ethyl methanesulfonate (EMS) damages has been investigated in the fungus Aspergillus nidulans. 4 UV-sensitive mutants, namely uvsB, uvsD, uvsF and uvsH have been tested for their sensitivity and mutability to the above-mentioned agents. The results obtained show that: (1) uvsB and uvsD mutants are no more sensitive than the wild-type strain to the lethal action of EMS. In contrast, they are more sensitive to MMS; (2) uvsF and uvsH mutants are more sensitive than the wild type to EMS at 37 degrees C but not at 20 degrees C. However, they are more sensitive than the wild type to MMS at 37 degrees C as well as at 20 degrees C; (3) the mutation frequencies after treatment with either MMS or EMS plotted against survival are not altered in the UV-sensitive strains compared to the wild-type strain. From these data it may be concluded that the repair of lethal lesions induced by ethylating and methylating agents is under the control of different pathways. Furthermore the mutants tested are not involved in the mutagenic process.

  8. UV laser ablation of silicon carbide ring surfaces for mechanical seal applications

    Science.gov (United States)

    Daurelio, Giuseppe; Bellosi, Alida; Sciti, Diletta; Chita, Giuseppe; Allegretti, Didio; Guerrini, Fausto

    2000-02-01

    Silicon carbide ceramic seal rings are treated by KrF excimer laser irradiation. Surface characteristics, induced by laser treatment, depend upon laser fluence, the number of laser pulses, their energy and frequency, the rotation rate of the ring and the processing atmosphere. It was ascertained that silicon carbide has to be processed under an inert atmosphere to avoid surface oxidation. Microstructural analyses of surface and cross section of the laser processed samples showed that the SiC surface is covered by a scale due to the melting/resolidification processes. At high fluence there are no continuous scales on the surfaces; materials is removed by decomposition/vaporization and the ablation depth is linearly dependent on the number of pulses. Different surface morphologies are observed. The evolution of surface morphology and roughness is discussed with reference to compositions, microstructure and physical and optical properties of the ceramic material and to laser processing parameters. Preliminary results on tribological behavior of the treated seals are reported.

  9. Semi-conservative synthesis of DNA in UV-sensitive mutant cells of Chinese hamster after UV-irradiation

    International Nuclear Information System (INIS)

    Vikhanskaya, F.L.; Khrebtukova, I.A.; Manuilova, E.S.

    1985-01-01

    A study was made of the rate of semi-conservative DNA synthesis in asynchronous UV-resistant (clone V79) and UV-sensitive clones (VII and XII) of Chinese hamster cells after UV-irradiation. In all 3 clones studied, UV-irradiation (5-30 J/m 2 ) induced a decrease in the rate of DNA synthesis during the subsequent 1-2 h. In the resistant clone (V79) recovery of DNA synthesis rate started after the first 2 h post-irradiation (5 J/m 2 ) and by the 3rd hour reached its maximum value, which constituted 70% of that observed in control, non-irradiated cells. The UV-sensitive mutant clones VII and XII showed no recovery in the rate of DNA synthesis during 6-7 h post-irradiation. The results obtained show that the survival of cells is correlated with the ability of DNA synthesis to recover after UV-irradiation in 3 clones studied. The observed recovery of UV-inhibited DNA synthesis in mutant clones may be due to certain defects in DNA repair. (orig.)

  10. The Fly Sensitizing Pigment Enhances UV Spectral Sensitivity While Preventing Polarization-Induced Artifacts

    Directory of Open Access Journals (Sweden)

    Marko Ilić

    2018-02-01

    Full Text Available Microvillar photoreceptors are intrinsically capable of detecting the orientation of e-vector of linearly polarized light. They provide most invertebrates with an additional sensory channel to detect important features of their visual environment. However, polarization sensitivity (PS of photoreceptors may lead to the detection of polarization-induced false colors and intensity contrasts. Most insect photoreceptors are thus adapted to have minimal PS. Flies have twisted rhabdomeres with microvilli rotated along the length of the ommatidia to reduce PS. The additional UV-absorbing sensitizing pigment on their opsin minimizes PS in the ultraviolet. We recorded voltage from Drosophila photoreceptors R1–6 to measure the spectral dependence of PS and found that PS in the UV is invariably negligible but can be substantial above 400 nm. Using modeling, we demonstrate that in R1–6 without the sensitizing pigment, PS in the UV (PSUV would exceed PS in the visible part of the spectrum (PSVIS by a factor PSUV/PSVIS = 1.2–1.8, as lower absorption of Rh1 rhodopsin reduces self-screening. We use polarimetric imaging of objects relevant to fly polarization vision to show that their degree of polarization outdoors is highest in the short-wavelength part of the spectrum. Thus, under natural illumination, the sensitizing pigment in R1–6 renders even those cells with high PS in the visible part unsuitable for proper polarization vision. We assume that fly ventral polarization vision can be mediated by R7 alone, with R1–6 serving as an unpolarized reference channel.

  11. Postirradiation properties of a UV-sensitive variant of CHO

    Energy Technology Data Exchange (ETDEWEB)

    Wood, R.D.; de Veciana, M.; Presson-Tincknell, B. (California Univ., Berkeley (USA). Lawrence Berkeley Lab.)

    1982-08-01

    A UV-hypersensitive mutant of Chinese hamster ovary (CHO) cells, termed 43-3B, has been used in a comparative study with the wild type CHO in order to determine the involvement of repair in several postirradiation phenomena. 43-3B has the same growth rate and chromosome number as the wild type CHO-9. It is hypersensitive to UV irradiation. 43-3B shows only about 17% of the UV-stimulated unscheduled DNA repair synthesis of CHO-9 as measured by autoradiography. When breaks in supercoiled chromatin are measured after UV by the nucleoid sedimentation method, the mutant appears to be capable of carrying out only limited incision. A much reduced ability to recover control rates of semiconservative DNA synthesis after UV irradiation was observed in the repair-deficient 43-3B cell line, suggesting that the removal of UV-induced replication blocks by excision repair is the most important factor in allowing recovery of UV-inhibited DNA synthesis. Recovery of colony-forming ability between fractionated UV exposures was observed in the wild type CHO-9, but little recovery was seen in 43-3B. This indicates that excision repair capability can also be important in split-fluence recovery.

  12. Diversity in UV sensitivity and recovery potential among bacterioneuston and bacterioplankton isolates.

    Science.gov (United States)

    Santos, A L; Lopes, S; Baptista, I; Henriques, I; Gomes, N C M; Almeida, A; Correia, A; Cunha, A

    2011-04-01

    To assess the variability in UV-B (280-320 nm) sensitivity of selected bacterial isolates from the surface microlayer and underlying water of the Ria de Aveiro (Portugal) estuary and their ability to recover from previous UV-induced stress. Bacterial suspensions were exposed to UV-B radiation (3·3 W m⁻²). Effects on culturability and activity were assessed from colony counts and (3) H-leucine incorporation rates, respectively. Among the tested isolates, wide variability in UV-B-induced inhibition of culturability (37·4-99·3%) and activity (36·0-98·0%) was observed. Incubation of UV-B-irradiated suspensions under reactivating regimes (UV-A, 3·65 W m⁻²; photosynthetic active radiation, 40 W m⁻²; dark) also revealed diversity in the extent of recovery from UV-B stress. Trends of enhanced resistance of culturability (up to 15·0%) and enhanced recovery in activity (up to 52·0%) were observed in bacterioneuston isolates. Bacterioneuston isolates were less sensitive and recovered more rapidly from UV-B stress than bacterioplankton isolates, showing enhanced reduction in their metabolism during the irradiation period and decreased culturability during the recovery process compared to bacterioplankton. UV exposure can affect the diversity and activity of microbial communities by selecting UV-resistant strains and alter their metabolic activity towards protective strategies. © 2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology.

  13. Expression of UV-Sensitive Parapinopsin in the Iguana Parietal Eyes and Its Implication in UV-Sensitivity in Vertebrate Pineal-Related Organs

    Science.gov (United States)

    Wada, Seiji; Kawano-Yamashita, Emi; Koyanagi, Mitsumasa; Terakita, Akihisa

    2012-01-01

    The pineal-related organs of lower vertebrates have the ability to discriminate different wavelengths of light. This wavelength discrimination is achieved through antagonistic light responses to UV or blue and visible light. Previously, we demonstrated that parapinopsin underlies the UV reception in the lamprey pineal organ and identified parapinopsin genes in teleosts and frogs of which the pineal-related organs were reported to discriminate light. In this study, we report the first identification of parapinopsin in the reptile lineage and show its expression in the parietal eye of the green iguana. Spectroscopic analysis revealed that iguana parapinopsin is a UV-sensitive pigment, similar to lamprey parapinopsin. Interestingly, immunohistochemical analyses using antibodies specific to parapinopsin and parietopsin, a parietal eye green-sensitive pigment, revealed that parapinopsin and parietopsin are colocalized in the outer segments of the parietal eye photoreceptor cells in iguanas. These results strongly suggest that parapinopsin underlies the wavelength discrimination involving UV reception in the iguana parietal eye. The current findings support the idea that parapinopsin is a common photopigment underlying the UV-sensitivity in wavelength discrimination of the pineal-related organs found from lampreys to reptiles. PMID:22720013

  14. Expression of UV-sensitive parapinopsin in the iguana parietal eyes and its implication in UV-sensitivity in vertebrate pineal-related organs.

    Science.gov (United States)

    Wada, Seiji; Kawano-Yamashita, Emi; Koyanagi, Mitsumasa; Terakita, Akihisa

    2012-01-01

    The pineal-related organs of lower vertebrates have the ability to discriminate different wavelengths of light. This wavelength discrimination is achieved through antagonistic light responses to UV or blue and visible light. Previously, we demonstrated that parapinopsin underlies the UV reception in the lamprey pineal organ and identified parapinopsin genes in teleosts and frogs of which the pineal-related organs were reported to discriminate light. In this study, we report the first identification of parapinopsin in the reptile lineage and show its expression in the parietal eye of the green iguana. Spectroscopic analysis revealed that iguana parapinopsin is a UV-sensitive pigment, similar to lamprey parapinopsin. Interestingly, immunohistochemical analyses using antibodies specific to parapinopsin and parietopsin, a parietal eye green-sensitive pigment, revealed that parapinopsin and parietopsin are colocalized in the outer segments of the parietal eye photoreceptor cells in iguanas. These results strongly suggest that parapinopsin underlies the wavelength discrimination involving UV reception in the iguana parietal eye. The current findings support the idea that parapinopsin is a common photopigment underlying the UV-sensitivity in wavelength discrimination of the pineal-related organs found from lampreys to reptiles.

  15. Expression of UV-sensitive parapinopsin in the iguana parietal eyes and its implication in UV-sensitivity in vertebrate pineal-related organs.

    Directory of Open Access Journals (Sweden)

    Seiji Wada

    Full Text Available The pineal-related organs of lower vertebrates have the ability to discriminate different wavelengths of light. This wavelength discrimination is achieved through antagonistic light responses to UV or blue and visible light. Previously, we demonstrated that parapinopsin underlies the UV reception in the lamprey pineal organ and identified parapinopsin genes in teleosts and frogs of which the pineal-related organs were reported to discriminate light. In this study, we report the first identification of parapinopsin in the reptile lineage and show its expression in the parietal eye of the green iguana. Spectroscopic analysis revealed that iguana parapinopsin is a UV-sensitive pigment, similar to lamprey parapinopsin. Interestingly, immunohistochemical analyses using antibodies specific to parapinopsin and parietopsin, a parietal eye green-sensitive pigment, revealed that parapinopsin and parietopsin are colocalized in the outer segments of the parietal eye photoreceptor cells in iguanas. These results strongly suggest that parapinopsin underlies the wavelength discrimination involving UV reception in the iguana parietal eye. The current findings support the idea that parapinopsin is a common photopigment underlying the UV-sensitivity in wavelength discrimination of the pineal-related organs found from lampreys to reptiles.

  16. Sensitivity of pathogenic and free-living Leptospira spp. to UV radiation and mitomycin C

    International Nuclear Information System (INIS)

    Stamm, L.V.; Charon, N.W.

    1988-01-01

    The habitats for the two major Leptospira spp. differ. The main habitat of L. biflexa is soil and water, whereas L. interrogans primarily resides in the renal tubules of animals. We investigated whether these two species, along with L. illini (species incertae sedis), differ with respect to their sensitivity to UV radiation. The doses of UV resulting in 37, 10 and 1% survival were determined for representive serovars from each species. L. interrogans serovar pomona was 3.0 to 4.8 times more sensitive to UV than the other Leptospira species under the 37, 10, and 1% survival parameters. In comparison to other bacteria, L. interrogans serovar pomona is among the most sensitive to UV. In a qualitative UV sensitivity assay., L. interrogans serovars were found to be in general more sensitive than L. biflexa serovars. All three species were found to have a photoreactivation DNA repair mechanism. Since organisms that are resistant to UV are often resistant to the DNA cross-linking agent mitomycin C, we tested the relative sensitivity of several Leptospira serovars to this compound. With few exceptions, L. biflexa and L. illini serovars were considerably more resistant to mitomycin C than the L. interrogans serovars. The mitomycin C sensitivity assay could be a useful addition to current characterization tests used to differentiate the Leptospira species

  17. Sensitivity of pathogenic and free-living Leptospira spp. to UV radiation and mitomycin C

    Energy Technology Data Exchange (ETDEWEB)

    Stamm, L.V.; Charon, N.W.

    1988-03-01

    The habitats for the two major Leptospira spp. differ. The main habitat of L. biflexa is soil and water, whereas L. interrogans primarily resides in the renal tubules of animals. We investigated whether these two species, along with L. illini (species incertae sedis), differ with respect to their sensitivity to UV radiation. The doses of UV resulting in 37, 10 and 1% survival were determined for representive serovars from each species. L. interrogans serovar pomona was 3.0 to 4.8 times more sensitive to UV than the other Leptospira species under the 37, 10, and 1% survival parameters. In comparison to other bacteria, L. interrogans serovar pomona is among the most sensitive to UV. In a qualitative UV sensitivity assay., L. interrogans serovars were found to be in general more sensitive than L. biflexa serovars. All three species were found to have a photoreactivation DNA repair mechanism. Since organisms that are resistant to UV are often resistant to the DNA cross-linking agent mitomycin C, we tested the relative sensitivity of several Leptospira serovars to this compound. With few exceptions, L. biflexa and L. illini serovars were considerably more resistant to mitomycin C than the L. interrogans serovars. The mitomycin C sensitivity assay could be a useful addition to current characterization tests used to differentiate the Leptospira species.

  18. Cyclobutyl pyrimidine dimer accumulation in relation to UV-B sensitivity in mung bean cultivars

    International Nuclear Information System (INIS)

    He Junmin; Wang Ruibin; Meng Zhaoni

    2006-01-01

    The purpose of the experiment is to reveal the relationship between cyclobutyl pyrimidine dimers (CPDs) accumulation and UV-B sensitivity in mung bean cultivars. Two mung bean cultivars (Phaseolus raditus L. cv. Qindou -2 0 and Zhonglü -1 ) were grown in greenhouse with treatment or without treatment of UV-B radiation (0.4 W * m -2 ) for four days. The UV-B-induced CPDs in mung bean DNA were quantified by enzyme-linked immunosorbent assay (ELISA) with specific monoclonal antibody and the relationship between CPDs accumulation and the UV-B sensitivity of mung bean seedlings were discussed. The UV-B-induced inhibition of the biomass and net photosynthetic rate of the primary leaves of cultivar Zhonglü -1 were respectively lower than that of cultivar Qindou -2 0, so that the cultivar Zhonglü -1 was more tolerant to UV-B than the cultivar Qindou -2 0. Meanwhile, the cultivar Zhonglü -1 had lower CPD accumulation and susceptibility to CPD induction, higher photorepair capacity and same dark repair capacity as compared with the cultivar Qindou -2 0. Different UV-B sensitivities between two mung bean cultivars may be mainly caused by the differences in CPD accumulation, which are caused by the different susceptibility to CPD induction and the different photorepair capacities. In addition, the different susceptibility to CPD induction between two mung bean cultivars is related to the different levels of UV-absorbing compounds

  19. Isolation of uv-sensitive variants of CHO-Kl by nylon cloth replicaplating

    International Nuclear Information System (INIS)

    Stamato, T.D.; Waldren, C.A.

    1977-01-01

    Techniques are described which permit the identification and isolation of uv-sensitive variants from mutagenized populations of Chinese hamster ovary (CHO) cells. Identification is based on the observation that within two days after receiving a dose of approximately 240 ergs/mm 2 of uv irradiation, most of the cells in a colony of CHO detach from the surface of a plastic tissue culture dish. At a lower dose of uv, which does not kill or detach a significant number of parental cells, uv-sensitive colonies are killed and become detached. Thus a clear plaque is produced in a lawn of unirradiated parental cells, marking the site occupied by a sensitive colony. Live cells from such sensitive colonies have been recovered from a nylon cloth replica prepared prior to irradiation and characterized. One uv-sensitive variant (CHO-UV-1) is indistinguishable from parental cells in x-ray resistance, chromosome number, generation time, and duration of the phases of the cell cycle

  20. Sensitization to UV-induced apoptosis by the histone deacetylase inhibitor trichostatin A (TSA)

    International Nuclear Information System (INIS)

    Kim, Myoung Sook; Baek, Jin Hyen; Chakravarty, Devulapalli; Sidransky, David; Carrier, France

    2005-01-01

    UV-induced apoptosis is a protective mechanism that is primarily caused by DNA damage. Cyclobutane pyrimidine dimers (CPD) and 6-4 photoproducts are the main DNA adducts triggered by UV radiation. Because the formation of DNA lesions in the chromatin is modulated by the structure of the nucleosomes, we postulated that modification of chromatin compaction could affect the formation of the lesions and consequently apoptosis. To verify this possibility we treated human colon carcinoma RKO cells with the histone deacetylase inhibitor trichostatin A (TSA) prior to exposure to UV radiation. Our data show that pre-treatment with TSA increased UV killing efficiency by more than threefold. This effect correlated with increased formation of CPDs and consequently apoptosis. On the other hand, TSA treatment after UV exposure rather than before had no more effect than UV radiation alone. This suggests that a primed (opened) chromatin status is required to sensitize the cells. Moreover, TSA sensitization to UV-induced apoptosis is p53 dependent. p53 and acetylation of the core histones may thus contribute to UV-induced apoptosis by modulating the formation of DNA lesions on chromatin

  1. Isolation of UV-sensitive mutants of mouse L5178Y cells by a cell suspension spotting method

    International Nuclear Information System (INIS)

    Shiomi, T.; Hieda-Shiomi, N.; Sato, K.

    1982-01-01

    We have isolated 56 UV-sensitive mutant clones from a mouse L51 T/t line of L5178Y cells by a cell suspension spotting method. Five mutants have also been isolated from L51 T/t and L5178Y cells by the method reported by Thompson and coworkers. We divided the mutants into two groups, highly sensitive and moderately sensitive mutants, according to their sensitivity to UV irradiation. Fifty-eight mutants were highly sensitive and three were moderately sensitive to UV. The reconstruction experiments indicate that more than 90% of highly sensitive mutants were recovered by the cell suspension spotting method. Frequencies of recovered mutants highly sensitive to UV increased with increasing dose of mutagens. Recovered mutant frequency reached 10(-2) after treatment with 1.5 micrograms/ml of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) (survival 0.2%). Eight UV-sensitive mutants were divided into four complementation groups. These mutants were 2-6 times more sensitive to UV than parental L51 T/t cells in terms of D37 (dose required to reduce survival to 37%). Four representative UV-sensitive mutants which are classified into different complementation groups were examined for their sensitivity to killing by UV, 4-nitroquinoline-1-oxide (4NQO), mitomycin C (MMC), X-rays, and MNNG. All four classes of mutants were found to be cross-sensitive to UV, 4NQO, and MMC, but not sensitive to X-rays and MNNG

  2. Thymidine kinase deficient human cells have increased UV sensitivity in their capacity to support herpes simplex virus but normal UV sensitivity for colony formation

    International Nuclear Information System (INIS)

    Rainbow, A.J.

    1989-01-01

    A thymidine kinase deficient (tk - ) and two thymidine kinase proficient (tk + ) human cell lines were compared for UV sensitivity using colony-forming ability as well as their capacity to support the plaque formation of herpes simplex type 1 (HSV-1).The tk - line (143 cells) was a derivative of one of the tk + lines (R970-5), whereas the other tk + line (AC4 cells) was a derivative of the 143 cells obtained by transfection with purified sheared HSV-2 DNA encoding the viral tk gene. 143, R970-5 and AC4 cells showed a similar UV sensitivity for colony-forming ability. In contrast, the capacity to support HSV-1 plaque formation immediately (within 1 h) afte UV-irradiation was reduced to a greater extent in the 143 cells compared to the R970-5 and AC4 cells. Capacity curves for plaque formation of the HSV-1: KOS wild-type (tk + ) strain were similar to those for the HSV-1: PTK3B mutant (tk - ) strain were similar to those for the HSV-1: PTK3B mutant (tk - ) strain in the 3 cell strains, indicating that the viral tk gene does not influence the ability of HSV-1 to form plaques in UV-irradiated compared to unirradiated human cells. Cellular capacity for HSV-1 plaque formation was found to recover in both tk - and tk + cells for cultures infected 24 h after UV-irradiation. These results suggest that repair of UV-damaged DNA takes place to a similar extent in both tk - and tk + human cells, but the kinetics of repair are initially slower in tk - compared to tk + human cells. (author). 33 refs.; 3 figs.; 1 tab

  3. Sensitivity Analysis of Core Damage from Reactor Coolant Pump Seal Leakage during Extended Loss of All AC Power

    Energy Technology Data Exchange (ETDEWEB)

    Park, Da Hee; Kim, Min Gi; Lee, Kyung Jin; Hwang, Su hyun; Lee, Byung Chul [FNC Technology Co. Ltd., Yongin (Korea, Republic of); Yoon, Duk Joo; Lee, Seung Chan [Korea Hydro and Nuclear Power Co. Ltd., Daejeon (Korea, Republic of)

    2015-10-15

    In this study, in order to comprehend the Fukushima accident, the sensitivity analysis was performed to analyze the behavior of Reactor Coolant System (RCS) during ELAP using the RELAP5/MOD3.3 code. The Fukushima accident was caused by tsunami resulted in Station Black Out (SBO) followed by the reactor core melt-down and release of radioactive materials. After the accident, the equipment and strategies for the Extended Loss of All AC Power (ELAP) were recommended strongly. In this analysis, sensitivity studies for the RCP seal failure of the OPR1000 type NPP were performed by using RELAP5/MOD3.3 code. Six cases with different leakage rate of RCP seal were studied for ELAP with operator action or not. The main findings are summarized as follows: (1) Without the operator action, the core uncovery time is determined by the leakage rate of RCP seal. When the leakage rate per RCP seal are 5 gpm, 50 gpm, and 300 gpm respectively, the core uncovery time are 1.62 hr, 1.58 hr, and 1.29 hr respectively. Namely, If the leakage rate of RCP seal was much bigger, the uncover time of core would be shorter. (2) In case that the cooling by SG secondary side was performed using the TDAFP and SG ADV, the core uncovery time was significantly extended.

  4. UV and gamma-ray sensitivity of meiosis-deficient mutants in Podospora anserina

    International Nuclear Information System (INIS)

    Simonet, J.M.

    1976-01-01

    Two mutants, mei1 and mei2, were isolated by screening for deficiencies occurring in the meiotic process. The sensitivity of mei1 and mei2 mutant strains to UV irradiation showed a significant increase as compared with that of the wild-type stock, hwhereas the sensitivity to γ-rays remained unchanged. The double-mutant strains were no more sensitive than each single mutant. The data indicate that both mei1 and mei2 loci are probably involved in the same pathway of excision-repair of UV-induced lesions

  5. Mutagenesis at the ad-3A and ad-3B loci in haploid UV-sensitive strains of Neurospora crassa. Pt. 2

    International Nuclear Information System (INIS)

    Serres, F.J. de

    1980-01-01

    UV-induced inactivation and induction of mutations at the ad-3A and ad-3B loci of Neurospora crassa have been compared among 7 different UV-sensitive strains and a standard wild-type strain. The 7 strains show varying degrees of sensitivity to UV-induced inactivation, with the relative sensitivity being: uvs-2 > uvs-3 > uvs-4 > uvs-6 > upr-1 > uvs-5 > uvs-1. Studies on the induction of ad-3 mutants by UV show that the 2 excision-repair deficient mutants uvs-2 and upr-1 exhibit enhanced ad-3 mutant frequencies, while uvs-4 and uvs-5 exhibit reduced ad-3 mutant frequencies, and uvs-3 completely eliminates UV mutagenesis. The ad-3 mutation-induction curves obtained with uvs-1 or uvs-6 are not significantly different from that found with the wild-type strain. (orig.)

  6. Meiotic UV-sensitive mutant that causes deletion of duplications in neurospora

    International Nuclear Information System (INIS)

    Newmeyer, D.; Galeazzi, D.R.

    1978-01-01

    The meiotic-3 (mei-3) mutant of Neurospora crassa has several effects: (1) when homozygous, it almost completely blocks meiosis and ascospore formation, (2) it is sensitive to uv, (3) its growth is inhibited by histidine, and (4) it increases the instability of nontandem duplications. This was shown for duplications produced by five different rearrangements and was demonstrated by two different criteria. The effects on meiosis and duplication instability are expressed strongly at 25 0 ; the effects on sensitivity to uv and to histidine are expressed strongly at 38.5 0 but only slightly at 25 0 . Nevertheless, all four effects were shown to be due to a single gene. Mei-3 is not allelic with previously reported uv-sensitive mutants. Two other results were obtained that are not necessarily due to mei-3: (1) a cross involving mei-3 produced a new unlinked meiotic mutant, mei-4, which is not sensitive to uv or histidine, and (2) a burst of several new mutants occurred in a different mei-3 stock, including a partial revertant to mei-3. Mei-3 has previously been shown to cause frequent complete loss of a terminal duplicate segment, beginning exactly at the original rearrangement breakpoint. Possible mechanisms are discussed by which a uv-sensitive mutant could cause such precise deletions

  7. Highly Sensitive Detection of UV Radiation Using a Uranium Coordination Polymer.

    Science.gov (United States)

    Liu, Wei; Dai, Xing; Xie, Jian; Silver, Mark A; Zhang, Duo; Wang, Yanlong; Cai, Yawen; Diwu, Juan; Wang, Jian; Zhou, Ruhong; Chai, Zhifang; Wang, Shuao

    2018-02-07

    The accurate detection of UV radiation is required in a wide range of chemical industries and environmental or biological related applications. Conventional methods taking advantage of semiconductor photodetectors suffer from several drawbacks such as sophisticated synthesis and manufacturing procedure, not being able to measure the accumulated UV dosage as well as high defect density in the material. Searching for new strategies or materials serving as precise UV dosage sensor with extremely low detection limit is still highly desirable. In this work, a radiation resistant uranium coordination polymer [UO 2 (L)(DMF)] (L = 5-nitroisophthalic acid, DMF = N,N-dimethylformamide, denoted as compound 1) was successfully synthesized through mild solvothermal method and investigated as a unique UV probe with the detection limit of 2.4 × 10 -7 J. On the basis of the UV dosage dependent luminescence spectra, EPR analysis, single crystal structure investigation, and the DFT calculation, the UV-induced radical quenching mechanism was confirmed. Importantly, the generated radicals are of significant stability which offers the opportunity for measuring the accumulated UV radiation dosage. Furthermore, the powder material of compound 1 was further upgraded into membrane material without loss in luminescence intensity to investigate the real application potentials. To the best of our knowledge, compound 1 represents the most sensitive coordination polymer based UV dosage probe reported to date.

  8. Abnormal sensitivity of some Cockayne's syndrome cell strains to UV- and γ-rays

    International Nuclear Information System (INIS)

    Deschavanne, P.J.; Chavaudra, N.; Fertil, B.; Malaise, E.P.

    1984-01-01

    Cockayne's syndrome (CS) is a rare autosomal recessive genetic disease. Using a colony assay in vitro, we studied the sensitivity of 5 CS cell strains and two normal ones to UV- and γ-irradiation. The 5 CS strains appear to be UV-hypersensitive but the sensitivity varies widely from one strain to another. Hypersensitivity to γ-rays has been reported for 4 out of the 5 CS cell strains investigated. Repair of potentially lethal damage (PLD) after UV- and γ-irradiation was investigated by using unfed plateau cell cultures. Under these conditions, control cells show a great capacity to repair PLD. The two CS strains, which are hypersensitive to both UV- and γ-irradiation, exhibit no or only little PLD repair after treatment. The response of CS cell strains after γ-irradiation suggests a genetic heterogeneity. Three complementation groups are described in CS cells when dealing with UV radiosensitivity. However, variations in γ-ray sensitivity are reported for cells within the same UV complementation group. (Auth.)

  9. Human chromosome 9 can complement UV sensitivity of xeroderma pigmentosum group A cells

    International Nuclear Information System (INIS)

    Ishizaki, Kanji; Sasaki, Masao S.; Ikenaga, Mituo; Nakamura, Yusuke

    1990-01-01

    A single human chromosome derived from normal human fibroblasts and tagged with the G418 resistance gene was transferred into SV40-transformed xeroderma pigmentosum group A (XP-A) cells via microcell fusion. When chromosome 1 or 12 was transferred, UV sensitivity of microcell hybrid cells was not changed. By contrast, after transferring chromosome 9,7 of 11 reipient clones were as UV-resistant as normal human cells. Four other clones were still as UV-sensitive as the parental XP-A cells. Southern hybridization analysis using a polymorphic probe, pEKZ19.3, which is homologous to a sequence of the D9S17 locus on chromosome 9, has confirmed that at least a part of normal human chromosome 9 was transferred into the recipient clones. However, amounts iof UV-induced unscheduled DNA synthesis in the UV-resistant clones were only one-third of those in normal human cells. These results indicate that a gene on chromosome 9 can confer complementation of high UV sensitivity of XP-A cells although it is still possible that 2 or more genes might be involved in the defective-repair phenotypes of XP-A. (author). 20 refs.; 3 figs.; 1 tab

  10. [Genetic control of the sensitivity of Aspergillus nidulans to mutagenic factors. VII. Inheritance of cross-sensitivity to different mutagenic factors by uvs-mutants].

    Science.gov (United States)

    Evseeva, G V; Kameneva, S V

    1977-01-01

    To study the inheritance of the sensitivity to UV, X-rays, methylmethanesulphonate (MMS), nitrosoguanidine (NG) and nitrous acid (NA) in five uvs mutants of Aspergillus nidulans, having multiple sensitivity to these factors, the sensitivity of recombinants obtained from crossing uvs mutants with uvs+ strain, resistant to all the factors analysed, and uvs leads to uvs+ revertants is investigated. Four uvs mutants (15, 17, 19 and 26) are found to have a nomogenic control of sensitivity to different mutagens. In one mutant (uvs11) the sensitivity to five factors is controlled by two non-linked mutations, one of them determining the sensitivity to UV, NG, NA, and the other--to X-rays and MMC. Phenotypic manifestations of uvs mutations is modified by cell genotype, both chromosomal and cytoplasmic factors being responsible for the modification. Phenotypic modification of uvs mutation results in the change to some (but not to all) mutagenic factors. It suggests, that not the product of uvs gene, but some other components of the reparation complex are modified. Otherwise, reparation of different DNA damages can be carried out by a single enzyme acting in different reparation complexes.

  11. Spontaneous mutation rate in Chinese hamster cell clones differing in UV-sensitivity

    International Nuclear Information System (INIS)

    Manuilova, E.S.; Bagrova, A.M.; Moskovskij Gosudarstvennyj Univ.

    1983-01-01

    The spontaneous rate of appearance of mutations to 6-mercaptopurine (6 MP) resistence in the cells of CHR2 and CHs2 clones dofferent in sensitivity to lethal and matagenous effect of UV-rays, is investigated. Increased UV-sensitivity of CHs2 clone is caused by the violation of postreplicative DNA reparation. It is established that the purity of spontaneously occuring mutations in both clones turns out to be similar, i.e. (1.5-1.8)x10 -5 for the cell pergeneration. It is shown that the effect of postreplicative DNA reparation in the cells of chinese hamster is not connected with the increase of spontaneous mutation ability. The problem on the possible role of reparation in the mechanism of appearance of spontaneous and induced mutations in the cells of Chinese hamster with increased UV-sensitivity is discussed

  12. UV beam shaper alignment sensitivity: grayscale versus binary designs

    Science.gov (United States)

    Lizotte, Todd E.

    2008-08-01

    What defines a good flat top beam shaper? What is more important; an ideal flat top profile or ease of alignment and stability? These are the questions designers and fabricators can not easily define, since they are a function of experience. Anyone can generate a theoretical beam shaper design and model it until it is clear that on paper the design looks good and meets the general needs of the end customer. However, the method of fabrication can add a twist that is not fully understood by either party until the beam shaper is actually tested for the first time in a system and also produced in high volume. This paper provides some insight into how grayscale and binary fabrication methods can produce the same style of beam shaper, with similar beam shaping performance; however provide a result wherein each fabricated design has separate degrees of sensitivity for alignment and stability. The paper will explain the design and fabrication approach for the two units and present alignment and testing data to provide a contrast comparison. Further data will show that over twenty sets of each fabricated design there is a consistency to the sensitivity issue. An understanding of this phenomenon is essential when considering the use of beam shapers on production equipment that is dedicated to producing micron-precision features within high value microelectronic and consumer products. We will present our findings and explore potential explanations and solutions.

  13. Sensitivity to UV radiation in early life stages of the Mediterranean sea urchin Sphaerechinus granularis (Lamarck)

    Energy Technology Data Exchange (ETDEWEB)

    Nahon, Sarah [UPMC Univ Paris 06, UMR 7621, LOBB, Observatoire Oceanologique, F-66651, Banyuls/mer (France); CNRS, UMR 7621, LOBB, Observatoire Oceanologique, F-66651, Banyuls/mer (France); Castro Porras, Viviana A. [UPMC Univ Paris 06, UMR 7621, LOBB, Observatoire Oceanologique, F-66651, Banyuls/mer (France); Pruski, Audrey M. [UPMC Univ Paris 06, UMR 7621, LOBB, Observatoire Oceanologique, F-66651, Banyuls/mer (France); CNRS, UMR 7621, LOBB, Observatoire Oceanologique, F-66651, Banyuls/mer (France); Charles, Francois [UPMC Univ Paris 06, UMR 7621, LOBB, Observatoire Oceanologique, F-66651, Banyuls/mer (France); CNRS, UMR 7621, LOBB, Observatoire Oceanologique, F-66651, Banyuls/mer (France)], E-mail: charles@obs-banyuls.fr

    2009-03-01

    The sea urchin Sphaerechinus granularis was used to investigate the impact of relevant levels of UV-B radiation on the early life stages of a common Mediterranean free spawning benthic species. Sperm, eggs and embryos were exposed to a range of UV radiation doses. The resulting endpoints were evaluated in terms of fertilisation success, development and survival rates. Above a weighted UV radiation dose of 0.0029 kJ m{sup -2}, fertilisation capability of irradiated sperm decreased rapidly. The exposure of the eggs to 0.0175 kJ m{sup -2} and more led to delayed and inhibited development with ensuing embryonic morphological abnormalities. One-day old larvae remained strongly sensitive to UV radiation as shown by the 50% decrease of the larval survival rate for a dose of 0.025 kJ m{sup -2} UVR. The elevated sensitivity of embryos to experimental UVR went along with a lack of significant amount of sunscreen compounds (e.g., mycosporine-like amino acids) in the eggs. The present results demonstrated that gamete viability and embryonic development may be significantly impaired by solar UV radiation in S. granularis, compromising in this way the reproduction of the species. Unless adaptive behavioural reproductive strategies exist, the influence of ambient UV radiation appears as a selective force for population dynamics of broadcast spawners in the shallow benthic Mediterranean environment.

  14. Sensitivity to UV radiation in early life stages of the Mediterranean sea urchin Sphaerechinus granularis (Lamarck)

    International Nuclear Information System (INIS)

    Nahon, Sarah; Castro Porras, Viviana A.; Pruski, Audrey M.; Charles, Francois

    2009-01-01

    The sea urchin Sphaerechinus granularis was used to investigate the impact of relevant levels of UV-B radiation on the early life stages of a common Mediterranean free spawning benthic species. Sperm, eggs and embryos were exposed to a range of UV radiation doses. The resulting endpoints were evaluated in terms of fertilisation success, development and survival rates. Above a weighted UV radiation dose of 0.0029 kJ m -2 , fertilisation capability of irradiated sperm decreased rapidly. The exposure of the eggs to 0.0175 kJ m -2 and more led to delayed and inhibited development with ensuing embryonic morphological abnormalities. One-day old larvae remained strongly sensitive to UV radiation as shown by the 50% decrease of the larval survival rate for a dose of 0.025 kJ m -2 UVR. The elevated sensitivity of embryos to experimental UVR went along with a lack of significant amount of sunscreen compounds (e.g., mycosporine-like amino acids) in the eggs. The present results demonstrated that gamete viability and embryonic development may be significantly impaired by solar UV radiation in S. granularis, compromising in this way the reproduction of the species. Unless adaptive behavioural reproductive strategies exist, the influence of ambient UV radiation appears as a selective force for population dynamics of broadcast spawners in the shallow benthic Mediterranean environment

  15. Hypermutability of a UV-sensitive aphidicolin-resistant mutant of Chinese hamster fibroblasts

    International Nuclear Information System (INIS)

    Liu, P.K.; Chang, C.; Trosko, J.E.

    1982-01-01

    An ultraviolet light (UV)-sensitive thymidine auxotroph of Chinese hamster V79 cells that exhibits pleiotropic effects such as a high level of deoxycytidine triphosphate, slow growth, sensitivity to cytidine, and high frequencies of site-specific bromodeoxyuridine-dependent chromosomal aberrations was selected by its resistance to aphidicolin. The UV-induced mutability of this mutant and one of its revertants, which retains some of the phenotypes listed above, was studied in 3 mutation assay systems. The results showed that the mutant was hypermutable for ouabain and diphtheria-toxin-resistant mutations compared to wild-type V79 cells at the same UV dose or the same survival level. The mutant exhibits a delayed expression of maximal frequency of induced 6-thioguanine-resistant mutants. When maximal frequencies are compared at the same UV dose, the mutant also has higher mutation frequencies at the hypoxanthine-guanine phosphoribosyl transferase locus. The revertant was similar to the wild-type in UV sensitivity and mutability. (orig./AJ)

  16. UV-sensitive photoreceptor protein OPN5 in humans and mice.

    Science.gov (United States)

    Kojima, Daisuke; Mori, Suguru; Torii, Masaki; Wada, Akimori; Morishita, Rika; Fukada, Yoshitaka

    2011-01-01

    A variety of animal species utilize the ultraviolet (UV) component of sunlight as their environmental cues, whereas physiological roles of UV photoreception in mammals, especially in human beings, remain open questions. Here we report that mouse neuropsin (OPN5) encoded by the Opn5 gene exhibited an absorption maximum (λmax) at 380 nm when reconstituted with 11-cis-retinal. Upon UV-light illumination, OPN5 was converted to a blue-absorbing photoproduct (λmax 470 nm), which was stable in the dark and reverted to the UV-absorbing state by the subsequent orange light illumination, indicating its bistable nature. Human OPN5 also had an absorption maximum at 380 nm with spectral properties similar to mouse OPN5, revealing that OPN5 is the first and hitherto unknown human opsin with peak sensitivity in the UV region. OPN5 was capable of activating heterotrimeric G protein Gi in a UV-dependent manner. Immuno-blotting analyses of mouse tissue extracts identified the retina, the brain and, unexpectedly, the outer ears as the major sites of OPN5 expression. In the tissue sections of mice, OPN5 immuno-reactivities were detected in a subset of non-rod/non-cone retinal neurons as well as in the epidermal and muscle cells of the outer ears. Most of these OPN5-immuno-reactivities in mice were co-localized with positive signals for the alpha-subunit of Gi. These results demonstrate the first example of UV photoreceptor in human beings and strongly suggest that OPN5 triggers a UV-sensitive Gi-mediated signaling pathway in the mammalian tissues.

  17. A UV-sensitive human clonal cell line, RSa, which has low repair activity

    International Nuclear Information System (INIS)

    Suzuki, N.; Fuse, A.

    1981-01-01

    The repair activity of a human transformed cell line, RSa, which was found to be highly sensitive to the lethal effects of 254 mm far-ultraviolet radiation, was compared with that of HeLa cells by evaluating the range of UV-induced incorporation of [methyl- 3 H]thymidine ([ 3 H]dThd) or 5-[6- 3 H]bromodeoxyuridine ([ 3 H]BrdUrd) into deoxyribonucleic acid. Direct scintillation counting was used for measuring the extent of unscheduled DNA synthesis (UDS) in UV-irradiated cells, which were treated with hydroxyurea or with arginine deprivation. More quantitative measurements were made by using the density labeling and equilibrium centrifugation method for assaying repair replication. All the amounts of UDS and repair replication in RSa cells were markedly below those in HeLa cells. The possible relationships of the low repair activity to abnormally high UV sensitivity in RSa cells are discussed. (orig.)

  18. UV-sensitive optical sensors based on ITO-gallium phosphide heterojunctions

    Energy Technology Data Exchange (ETDEWEB)

    Malik, Oleksandr; Hidalga-Wade, F. Javier de la; Zuniga-Islas, Carlos; Abundis Patino, Jesus H. [National Institute for Astrophysics, Optics, and Electronics (INAOE), Puebla (Mexico)

    2010-04-15

    Design and characteristics of wide-band UV sensors based on ITO/GaP heterostructures are discussed. Such sensors have perfect electrical parameters and high UV-visible sensitivity in comparison with surface-barrier structures using a semi-transparent thin metal film as an electrode. Many applications require UV sensors with an effective rejection of visible radiation and a wide temperature operating interval. For this aim, the theoretical modelling of extreme selective optical sensors with a double Ag/ITO thin film on the GaP surface, in which the thin silver film serves as a narrow bandpass filter at 320 nm, has been conducted. With this modelling the optimal thickness combination for the silver and ITO films was found for the maximum rejection of the sensitivity to visible radiation (copyright 2010 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  19. Comparative sensitivity of harbour and grey seals to several environmental contaminants using in vitro exposure

    International Nuclear Information System (INIS)

    Dufresne, M.M.; Frouin, H.; Pillet, S.; Lesage, V.; De Guise, S.; Fournier, M.

    2010-01-01

    In this study, we investigated the effects of cadmium chloride (CdCl 2 ), mercury chloride (HgCl 2 ), methylmercury chloride (CH 3 HgCl), and PCBs on lymphocyte proliferation in phocids. PBMCs isolated from harbour and grey seals were exposed in vitro to varying concentrations of contaminants. A reduction of viability occurred when cells were exposed to 10 -4 M HgCl 2 or CH 3 HgCl or to 50 ppm of Aroclor 1254. In both grey and harbour seals, T-lymphocyte proliferation was suppressed when their cells were incubated with 5 x 10 -5 M CdCl 2 or 10 -4 M HgCl 2 . An inhibition of proliferation occurred with CH 3 HgCl from 10 -6 M in grey seals and from 10 -5 M in harbour seals. In grey seals, Aroclor 1254 reduced lymphocyte proliferation at 15 ppm. In both harbour and grey seals, CH 3 HgCl was ten times more immunotoxic that HgCl 2 . From IC 50 , chemicals were ranked in terms of toxicity as followed: CH 3 HgCl > CdCl 2 > HgCl 2 > Aroclor 1254.

  20. Seals and sealing handbook

    CERN Document Server

    Flitney, Robert K

    2014-01-01

    Seals and Sealing Handbook, 6th Edition provides comprehensive coverage of sealing technology, bringing together information on all aspects of this area to enable you to make the right sealing choice. This includes detailed coverage on the seals applicable to static, rotary and reciprocating applications, the best materials to use in your sealing systems, and the legislature and regulations that may impact your sealing choices. Updated in line with current trends this updated reference provides the theory necessary for you to select the most appropriate seals for the job and with its 'Failur

  1. Occurrence and elimination of sites sensitive to UV-endonuclease in UV-irradiated E. coli cells

    Energy Technology Data Exchange (ETDEWEB)

    Kleibl, K; Sedliakova, M [Slovenska Akademia Vied, Bratislava (Czechoslovakia). Vyskumny Ustav Onkologicky

    1979-01-01

    The occurrence and elimination of sites sensitive to the endonucleolytic action of crude extract from M. luteus (Es sites) were studied in both the parental and daughter DNA of E. coli B/r Hcr/sup +/ irradiated either with lethal fluence only (LF) or with inducing and lethal fluence (IF+LF); after the lethal fluence protein synthesis could either take place or it was inhibited by chlorampehnicol (CAP). The data obtained showed that in the wild type UV-irradiated cells Es sites could be eliminated from their DNA molecules either through pyrimidine dimer excision or through the modification of dimers on replication. It appears that DNA repair takes place most efficiently in cells irradiated with IF+LF and postincubated with CAP; in these conditions cells are supplied with inducible proteins, and enough time for DNA repair is provided before the division of irradiated cells is resumed.

  2. Sensitivity of Vibrio cholerae cells to lethal and mutagenic effect of UV-irradiation mediated by plasmids

    International Nuclear Information System (INIS)

    Tiganova, I.G.; Evdokimova, N.M.; Aleshkin, G.I.

    1988-01-01

    The effect of UV-irradiation on Vibrio cholerae cells and its changes mediated by the plasmid R245 have been studied. Vibrio cholerae strains 569B and RV31 have been shown to be considerably more sensitive to lethal effect of UV-irradiation as compared with Escherichia coli and Salmonella typhimurium cells. Highly toxigenic strain 569B and practically atoxigenic strain RV31 have the same UV-sensitivity. Lethla effect of UV-irradiation on Vibrio cholerae cells is incresed when the irradiated cells are plated on enriched media. UV-induction of mutations was not registered in plasmidless strains of Vibrio cholerae. Plasmid R245 increase UV-resistance of vibrio cells and makes them UV-mutable

  3. Enhancing the far-UV sensitivity of silicon CMOS imaging arrays

    Science.gov (United States)

    Retherford, K. D.; Bai, Yibin; Ryu, Kevin K.; Gregory, J. A.; Welander, Paul B.; Davis, Michael W.; Greathouse, Thomas K.; Winter, Gregory S.; Suntharalingam, Vyshnavi; Beletic, James W.

    2014-07-01

    We report our progress toward optimizing backside-illuminated silicon PIN CMOS devices developed by Teledyne Imaging Sensors (TIS) for far-UV planetary science applications. This project was motivated by initial measurements at Southwest Research Institute (SwRI) of the far-UV responsivity of backside-illuminated silicon PIN photodiode test structures described in Bai et al., SPIE, 2008, which revealed a promising QE in the 100-200 nm range as reported in Davis et al., SPIE, 2012. Our effort to advance the capabilities of thinned silicon wafers capitalizes on recent innovations in molecular beam epitaxy (MBE) doping processes. Key achievements to date include: 1) Representative silicon test wafers were fabricated by TIS, and set up for MBE processing at MIT Lincoln Laboratory (LL); 2) Preliminary far-UV detector QE simulation runs were completed to aid MBE layer design; 3) Detector fabrication was completed through the pre-MBE step; and 4) Initial testing of the MBE doping process was performed on monitoring wafers, with detailed quality assessments. Early results suggest that potential challenges in optimizing the UV-sensitivity of silicon PIN type CMOS devices, compared with similar UV enhancement methods established for CCDs, have been mitigated through our newly developed methods. We will discuss the potential advantages of our approach and briefly describe future development steps.

  4. Repair of closely opposed cyclobutyl pyrimidine dimers in UV-sensitive human diploid fibroblasts

    International Nuclear Information System (INIS)

    Lam, L.H.; Reynolds, R.J.

    1986-01-01

    An enzyme-sensitive site assay has been used to examine the fate of closely opposed pyrimidine dimers in fibroblasts from individuals afflicted with various genetic disorders that confer increased cellular sensitivity to UV radiation. The disappearance of bifilar enzyme-sensitive sites was found to be normal in cells from individuals with Fanconi's anemia, Cockayne's syndrome, dyskeratosis congenita and the variant form of xeroderma pigmentosum. The rate of bifilar enzyme-sensitive site removal in XP cells assigned to complementation group C was reduced by an amount similar to that observed for the repair of isolated dimers. Our results indicate that the initiation of repair at closely opposed dimers is slow in XP-C cells but normal in all other cells examined. (Auth.)

  5. A UV-resistant mutant without an increased repair synthesis activity, established from a UV-sensitive human clonal cell line

    International Nuclear Information System (INIS)

    Suzuki, N.

    1984-01-01

    When cells of a human clonal cell line, RSa, with high sensitivity to UV lethality, were treated with the mutagen, ethyl methanesulfonate, a variant cell strain, UVr-1, was established as a mutant resistant to 254-nm far-ultraviolet radiation (UV). Cell proliferation studies showed that UVr-1 cells survived and actively proliferated at doses of UV-irradiation that greatly suppressed the proliferation of RSa cells. Colony-formation assays also confirmed the increased resistance of UVr-1 cells to UV. The recovery from a UV-induced inhibition in DNA synthesis, as [methyl- 3 H]thymidine uptake into cellular DNA, was more pronounced in UVr-1 cells than in RSa cells. Nevertheless, there was no significant difference in the activity of UV-induced DNA repair synthesis in either cell line, as estimated by the extent of unscheduled DNA synthesis and DNA repair replication. These characteristics of UVr-1 cells are discussed in the light of a previously reported UV-resistant variant, UVr-10, which had an increased DNA repair synthesis activity. (Auth.)

  6. Contribution of a caffeine-sensitive recombinational repair pathway to survival and mutagenesis in UV-irradiated Schizosaccharomyces pombe

    International Nuclear Information System (INIS)

    Gentner, N.E.; Werner, M.M.; Hannan, M.A.; Nasim, A.

    1978-01-01

    Cells of wild-type Schizosacharomyces pombe exposed to UV radiation in either G1 or G2 phase show enhanced inactivation of colony-forming ability if plated in the presence of caffeine. This UV-sensitization by caffeine is abolished in both G1 an G2 phase cells by the radlmutation; since both caffeine and the radl mutation markedly reduce recombinational events, this suggests that a recombinational repair process is active in cells irradiated either in G1 or G2 phase. Caffeine-sensitive repair begins immediately and is completed before resumption of DNA synthesis. Caffeine-sensitive repair of UV-damage in G1 cells displays a considerable lag and then occurs concomitantly with DNA synthesis. UV-induced mutagenesis was examined in wild-type and rad mutants using a forward mutation system. Rad mutants which show higher UV-induced mutation rates than wild-type retain the recombinational mechanism. In contrast, rad strains which are relatively UV-immutable compared to wild-type do not possess the caffeine-sensitive UV-repair process. The recombinational process therefore may be the major pathway responsible for UV-induced mutation. (orig./AJ) [de

  7. UV-B-inducible and temperature-sensitive photoreactivation of cyclobutane pyrimidine dimers in Arabidopsis thaliana

    International Nuclear Information System (INIS)

    Pang, Qishen; Hays, J.B.

    1991-01-01

    Removal of cyclobutane pyrimidine dimers (CBPDs) in vivo from the DNA of UV-irradiated eight-leaf seedlings of Arabidopsis thaliana was rapid in the presence of visible light (half-life about 1 hour); removal of CBPDs in the dark, presumably via excision repair, was an order of magnitude slower. Extracts of plants contained significant photolyase in vitro, as assayed by restoration of transforming activity to UV-irradiated Escherichia coli plasmids; activity was maximal from four-leaf to 12-leaf stages. UV-B treatment of seedlings for 6 hours increased photolyase specific activity in extracts twofold. Arabidopsis photolyase was markedly temperature-sensitive, both in vitro (half-life at 30C about 12 minutes) and in vivo (half-life at 30C, 30 to 45 minutes). The wavelength dependency of the photoreactivation cross-section showed a broad peak at 375 to 400 nm, and is thus similar to that for maize pollen; it overlaps bacterial and yeast photolyase action spectra

  8. Improvement of thermal stability of UV curable pressure sensitive adhesive by surface modified silica nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Pang, Beili; Ryu, Chong-Min; Kim, Hyung-Il, E-mail: hikim@cnu.ac.kr

    2013-11-01

    Highlights: • Silica nanoparticles were modified to carry the vinyl groups for photo-crosslinking. • Acrylic copolymer was modified to have the vinyl groups for photo-crosslinking. • Strong and extensive interfacial bondings were formed between polymer and silica. • Thermal stability of PSA was improved by forming nanocomposite with modified silica. -- Abstract: Pressure sensitive adhesives (PSAs) with higher thermal stability were successfully prepared by forming composite with the silica nanoparticles modified via reaction with 3-methacryloxypropyltrimethoxysilane. The acrylic copolymer was synthesized as a base resin for PSAs by solution polymerization of 2-EHA, EA, and AA with AIBN as an initiator. The acrylic copolymer was further modified with GMA to have the vinyl groups available for UV curing. The peel strength decreased with the increase of gel content which was dependent on both silica content and UV dose. Thermal stability of the composite PSAs was improved noticeably with increasing silica content and UV dose mainly due to the strong and extensive interfacial bonding between the organic polymer matrix and silica.

  9. Behaviour of UV-sensitive mutants of Proteus mirabilis to repair incision breaks

    International Nuclear Information System (INIS)

    Stoerl, K.; Mund, C.

    1977-01-01

    In U.V.-sensitive mutants of P. mirabilis with the phenotype HCR, REC and EXR single-strand breaks appeared immediately after UV-irradiation. The behaviour of REC- and EXR-mutants was similar to the wildtype. The number of incision breaks observed by sedimentation analysis in these strains was very low. They could be joined during the excision repair process. From the ability of REC- and EXR-strains to rejoin most of the induced single-strand breaks it can be concluded that these strains have approximately the same capacity for excision repair as the wildtype. HCR-mutants of P. mirabilis produced single-strand breaks after UV-irradiation in contrast to HCR-mutants of E. coli. Therefore we suggest that HCR-mutants of P. mirabilis are not completely inhibited in the incision step. The single-strand breaks introduced in the DNA at the beginning of the repair process were not rejoined during further incubation. Experiments with toluenized cells led to the same results. The newly synthesized daughter DNA-strands of UV-irradiated HCR-mutants were of low molecular weight in comparison with those from unirradiated control cells during the repair period. This result is in agreement with the incapability of HCR-mutants to remove the pyrimidine dimers from the parental template strand. (author)

  10. Mutagenesis at the ad-3A and ad-3B loci in haploid UV-sensitive strains of Neurospora crassa. Pt. 3

    International Nuclear Information System (INIS)

    Schuepbach, M.E.; Serres, F.J. de

    1981-01-01

    γ-ray-induced inactivation and induction of mutations at the ad-3A and ad-3B loci of Neurospora crassa have been compared among 6 different UV- sensitive strains and a standard wild-type strain. The 6 strains show varying degrees of sensitivy to γ-ray-induced inactivation, with the relative sensitivy at 37% survival being uvs-6 > upr-1 > uvs-2 UE uvs-3 > wild-type > uvs-5 > uvs-4. Studies on the induction of ad-3 mutants by γ-rays show that when the dose-response curves (expressed in terms of ad-3 mutants among the surving colonies) of the UV-sensitive strains are compared with wild-type, the excision-repair-deficient mutants uvs-2 and upr-1 exhibit enhanced ad-3 mutant frequencies, uvs-3 exibits reduced ad-3 mutant frequencies whereas both uvs-4 and uvs-5 show lower mutant frequencies than wild-type. (orig.)

  11. γ-irradiation resistance and UV-sensitivity of extremely thermophilic archebacteria and eubacteria

    International Nuclear Information System (INIS)

    Kopylov, V.M.; Bonch-Osmolovskaya, E.A.; Svetlichnyi, V.A.; Miroshnichenko, M.L.; Skobkin, V.S.

    1993-01-01

    Cells of extremely thermophilic sulfur-dependent archebacteria Desulfurococcus amylolyticus Z533 and Thermococcus stelleri K15 are resistant to γ-irradiation. These archebacteria survive γ-irradiation at a dose of up to 5 kGy but are no longer viable after 8-9 kGy. Comparison of the survival profiles showed that archebacteria are 12 to 25 times more resistant to γ-irradiation at moderate doses (LD 50 and LD 90 ) than E. coli K12 but are 2 to 2.5 times more sensitive than D. radiodurans. γ-irradiation at a dose of 1 to 2.5 kGy killed extremely thermophilic anaerobic eubacteria Thermotoga maritima 2706 and Thermodesulfobacterium P. All extreme thermophiles studied were more sensitive to UV-irradiation than E. coli

  12. Seals and sealing handbook

    CERN Document Server

    Flitney, Robert K

    2007-01-01

    Wherever machinery operates there will be seals of some kind ensuring that the machine remains lubricated, the fluid being pumped does not leak, or the gas does not enter the atmosphere. Seals are ubiquitous, in industry, the home, transport and many other places. This 5th edition of a long-established title covers all types of seal by application: static, rotary, reciprocating etc. The book bears little resemblance to its predecessors, and Robert Flitney has re-planned and re-written every aspect of the subject. No engineer, designer or manufacturer of seals can afford to be without this uniq

  13. Comparison of the reversibility of loci pet23 and lys2 after UV irradiation in the standard and UV-sensitive strains of Saccharomyces

    International Nuclear Information System (INIS)

    Vlckova, V.; Kovacova, V.

    1984-01-01

    Reversibility of the respiration-deficient locus pet23 and auxotrophic locus lys2 was followed in the standard (RAD1) and UV sensitive (rad1 to 2) strains of Saccharomyces cerevisiae, both after identical doses of UV radiation and at identical survival. By comparison of reversibility after treatment with identical doses of UV radiation a much higher reversibility of both loci in strain rad1 to 2 could be detected. A comparison of reversibility of the loci at identical survival of both strains showed that the reversibility of the pet23 locus is much higher in strain rad1 to 2, whereas reversibility of the lys2 locus is roughly identical in the two strains. Thus, the function of gene RAD1 in repair processes is apparently associated with ''error-free'' repair, both at low and high doses of ultraviolet radiation. (author)

  14. Sensitivity analysis on the interfacial drag in SPACE code to simulate UPTF separate effect test about loop seal clearance phenomenon

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Sukho; Lim, Sanggyu; You, Gukjong; Park, Youngsheop [Korea Hydro and Nuclear Power Company, Ltd., Daejeon (Korea, Republic of)

    2014-05-15

    The nuclear thermal hydraulic system code known as SPACE (Safety and Performance Analysis CodE) was developed and its V and V (Verification and Validation) have been conducted using well-known SETs (Separate Effect Tests) and IETs (Integral Effect Tests). At the same time, the SBLOCA (Small Break Loss of Coolant Accident) methodology in accordance with Appendix K of 10CFR50 for the APR1400 (Advanced Power Reactor 1400) was developed and applied to regulatory body for licensing in 2013. Especially, the SBLOCA methodology developed using SPACE v2.14 code adopts inherent test matrix independent of V and V test to show its conservatism for important phenomena. In this paper, the predictability of SPACE code for UPTF (Upper Plenum Test Facility) test simulating loop seal clearance of SBLOCA important phenomena and the related sensitivity analysis are introduced.

  15. Terbium fluorescence as a sensitive, inexpensive probe for UV-induced damage in nucleic acids

    International Nuclear Information System (INIS)

    El-Yazbi, Amira F.; Loppnow, Glen R.

    2013-01-01

    Graphical abstract: -- Highlights: •Simple, inexpensive, mix-and-read assay for positive detection of DNA damage. •Recognition of undamaged DNA via hybridization to a hairpin probe. •Terbium(III) fluorescence reports the amount of damage by binding to ssDNA. •Tb/hairpin is a highly selective and sensitive fluorescent probe for DNA damage. -- Abstract: Much effort has been focused on developing methods for detecting damaged nucleic acids. However, almost all of the proposed methods consist of multi-step procedures, are limited, require expensive instruments, or suffer from a high level of interferences. In this paper, we present a novel simple, inexpensive, mix-and-read assay that is generally applicable to nucleic acid damage and uses the enhanced luminescence due to energy transfer from nucleic acids to terbium(III) (Tb 3+ ). Single-stranded oligonucleotides greatly enhance the Tb 3+ emission, but duplex DNA does not. With the use of a DNA hairpin probe complementary to the oligonucleotide of interest, the Tb 3+ /hairpin probe is applied to detect ultraviolet (UV)-induced DNA damage. The hairpin probe hybridizes only with the undamaged DNA. However, the damaged DNA remains single-stranded and enhances the intrinsic fluorescence of Tb 3+ , producing a detectable signal directly proportional to the amount of DNA damage. This allows the Tb 3+ /hairpin probe to be used for sensitive quantification of UV-induced DNA damage. The Tb 3+ /hairpin probe showed superior selectivity to DNA damage compared to conventional molecular beacons probes (MBs) and its sensitivity is more than 2.5 times higher than MBs with a limit of detection of 4.36 ± 1.2 nM. In addition, this probe is easier to synthesize and more than eight times cheaper than MBs, which makes its use recommended for high-throughput, quantitative analysis of DNA damage

  16. The impact of short-term UV irradiation on grains of sensitive and tolerant cereal genotypes studied by EPR.

    Science.gov (United States)

    Kurdziel, Magdalena; Filek, Maria; Łabanowska, Maria

    2018-05-01

    UV irradiation has ionisation character and leads to the generation of reactive oxygen species (ROS). The destructive character of ROS was observed among others during interaction of cereal grains with ozone and was caused by changes in structures of biomolecules leading to the formation of stable organic radicals. That effect was more evident for stress sensitive genotypes. In this study we investigated the influence of UV irradiation on cereal grains originating from genotypes with different tolerance to oxidative stress. Grains and their parts (endosperm, embryo and seed coat) of barley, wheat and oat were subjected to short-term UV irradiation. It was found that UV caused the appearance of various kinds of reactive species (O 2 -• , H 2 O 2 ) and stable radicals (semiquinone, phenoxyl and carbon-centred). Simultaneously, lipid peroxidation occurred and the organic structure of Mn(II) and Fe(III) complexes become disturbed. UV irradiation causes damage of main biochemical structures of plant tissues, the effect is more significant in sensitive genotypes. In comparison with ozone treatment, UV irradiation leads to stronger destruction of biomolecules in grains and their parts. It is caused by the high energy of UV light, facilitating easier breakage of molecular bonds in biochemical compounds. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  17. A compromised yeast RNA polymerase II enhances UV sensitivity in the absence of global genome nucleotide excision repair.

    Science.gov (United States)

    Wong, J M; Ingles, C J

    2001-02-01

    Nucleotide excision repair is the major pathway responsible for removing UV-induced DNA damage, and is therefore essential for cell survival following exposure to UV radiation. In this report, we have assessed the contributions of some components of the RNA polymerase II (Pol II) transcription machinery to UV resistance in Saccharomyces cerevisiae. Deletion of the gene encoding the Pol II elongation factor TFIIS (SII) resulted in enhanced UV sensitivity, but only in the absence of global genome repair dependent on the RAD7 and RAD16 genes, a result seen previously with deletions of RAD26 and RAD28, yeast homologs of the human Cockayne syndrome genes CSB and CSA, respectively. A RAD7/16-dependent reduction in survival after UV irradiation was also seen in the presence of mutations in RNA Pol II that confer a defect in its response to SII, as well as with other mutations which reside in regions of the largest subunit of Pol II not involved in SII interactions. Indeed, an increase in UV sensitivity was achieved by simply decreasing the steadystate level of RNA Pol II. Truncation of the C-terminal domain and other RNA Pol II mutations conferred sensitivity to the ribonucleotide reductase inhibitor hydroxyurea and induction of RNR1 and RNR2 mRNAs after UV irradiation was attenuated in these mutant cells. That UV sensitivity can be a consequence of mutations in the RNA Pol II machinery in yeast cells suggests that alterations in transcriptional programs could underlie some of the pathophysiological defects seen in the human disease Cockayne syndrome.

  18. The sensitivity of sunflower (Helianthus annuus L. plants to UV-B radiation is altered by nitrogen status

    Directory of Open Access Journals (Sweden)

    Inês Cechin

    2018-02-01

    Full Text Available ABSTRACT: Interaction effects between nitrogen and UV-B radiation were studied in sunflower (Helianthus annuus L. variety IAC-Iarama plants grown in a greenhouse under natural photoperiod conditions. Plants were irradiated with 0.8W m-2 (control or 8.0W m-2 (+UV-B of UV-B radiation for 7h per day. The plants were grown in pots containing vermiculite and watered with 70% of full strength nitrogen-free Long Ashton solution, containing either low (42.3ppm or high (282ppm nitrogen as ammonium nitrate. High nitrogen increased dry matter of stem, leaves and shoot, photosynthetic pigments and photosynthesis (A without any alteration in stomatal conductance (gs nor transpiration (E while it reduced the intercellular CO2 (Ci concentration, and malondialdehyde (MDA content. High UV-B radiation had negative effects on dry matter production, A, gs and E with the effects more marked under high nitrogen, whereas it increased Ci under high nitrogen. Activity of PG-POD was reduced by high UV-B radiation under low nitrogen but it was not changed under high nitrogen. The UV-B radiation increased the MDA content independently of nitrogen level. Results indicate that the effects of UV-B radiation on sunflower plants are dependent of nitrogen supply with high nitrogen making their physiological processes more sensitive to UV-B radiation.

  19. Positive selection of a duplicated UV-sensitive visual pigment coincides with wing pigment evolution in Heliconius butterflies

    Science.gov (United States)

    Briscoe, Adriana D.; Bybee, Seth M.; Bernard, Gary D.; Yuan, Furong; Sison-Mangus, Marilou P.; Reed, Robert D.; Warren, Andrew D.; Llorente-Bousquets, Jorge; Chiao, Chuan-Chin

    2010-01-01

    The butterfly Heliconius erato can see from the UV to the red part of the light spectrum with color vision proven from 440 to 640 nm. Its eye is known to contain three visual pigments, rhodopsins, produced by an 11-cis-3-hydroxyretinal chromophore together with long wavelength (LWRh), blue (BRh) and UV (UVRh1) opsins. We now find that H. erato has a second UV opsin mRNA (UVRh2)—a previously undescribed duplication of this gene among Lepidoptera. To investigate its evolutionary origin, we screened eye cDNAs from 14 butterfly species in the subfamily Heliconiinae and found both copies only among Heliconius. Phylogeny-based tests of selection indicate positive selection of UVRh2 following duplication, and some of the positively selected sites correspond to vertebrate visual pigment spectral tuning residues. Epi-microspectrophotometry reveals two UV-absorbing rhodopsins in the H. erato eye with λmax = 355 nm and 398 nm. Along with the additional UV opsin, Heliconius have also evolved 3-hydroxy-DL-kynurenine (3-OHK)-based yellow wing pigments not found in close relatives. Visual models of how butterflies perceive wing color variation indicate this has resulted in an expansion of the number of distinguishable yellow colors on Heliconius wings. Functional diversification of the UV-sensitive visual pigments may help explain why the yellow wing pigments of Heliconius are so colorful in the UV range compared to the yellow pigments of close relatives lacking the UV opsin duplicate. PMID:20133601

  20. Cockayne's syndrome: correlation of clinical features with cellular sensitivity of RNA synthesis to UV irradiation

    International Nuclear Information System (INIS)

    Lehmann, A.R.; Thompson, A.F.; Harcourt, S.A.

    1993-01-01

    Cockayne's syndrome (CS) is a rare autosomal recessive disorder with dwarfism, mental retardation, and otherwise clinically heterogeneous features. In cultured CS fibroblasts, the failure of RNA synthesis to recover to normal rates after UV-C irradiation provides a useful and relatively simple diagnostic test. We have measured post-UV-C RNA synthesis in 52 patients for whom a clinical diagnosis of CS was considered a possibility. Twenty-nine patients showed the defect characteristic of CS cells, and 23 had a normal response. We have attempted to correlate the cellular diagnosis with the different clinical features of the disorder. Clinical details of the patients were obtained from referring clinicians in the form of a questionnaire. Our results show that, apart from the cardinal features of dwarfism and mental retardation, sun sensitivity correlated best with a positive cellular diagnosis. Pigmentary retinopathy, gait defects, and dental caries were also good positive indicators, although several patients with a positive cellular diagnosis did not have these features. (Author)

  1. A ΔdinB mutation that sensitizes Escherichia coli to the lethal effects of UV- and X-radiation

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Mei-Chong W.; Franco, Magdalena; Vargas, Doris M. [Department of Biological Sciences, San Jose State University, San Jose, CA 95192 (United States); Hudman, Deborah A. [Department of Microbiology and Immunology, A.T. Still University, Kirksville College of Osteopathic Medicine, Kirksville, MO 63501 (United States); White, Steven J. [Department of Biological Sciences, San Jose State University, San Jose, CA 95192 (United States); Fowler, Robert G., E-mail: rfowler@sjsu.edu [Department of Biological Sciences, San Jose State University, San Jose, CA 95192 (United States); Sargentini, Neil J. [Department of Microbiology and Immunology, A.T. Still University, Kirksville College of Osteopathic Medicine, Kirksville, MO 63501 (United States)

    2014-05-15

    Highlights: • We describe Δ(dinB-yafN)883(::kan), a novel dinB allele, referred to as ΔdinB883, a deletion that sensitizes E. coli cells to UV irradiation. • This UV radiation sensitivity is most acute in the early logarithmic phase of culture growth. • This UV radiation sensitivity is completely dependent upon a functional umuDC operon. • Sequencing reveals ΔdinB883 retains the proximal 161 nucleotides, i.e., 54 amino acids, of the wild-type sequence. • The ΔdinB883 mutant is hypothesized to produce a peptide of 83 amino acids, DinB883, that compromises UmuDC function. - Abstract: The DinB (PolIV) protein of Escherichia coli participates in several cellular functions. We investigated a dinB mutation, Δ(dinB-yafN)883(::kan) [referred to as ΔdinB883], which strongly sensitized E. coli cells to both UV- and X-radiation killing. Earlier reports indicated dinB mutations had no obvious effect on UV radiation sensitivity which we confirmed by showing that normal UV radiation sensitivity is conferred by the ΔdinB749 allele. Compared to a wild-type strain, the ΔdinB883 mutant was most sensitive (160-fold) in early to mid-logarithmic growth phase and much less sensitive (twofold) in late log or stationary phases, thus showing a growth phase-dependence for UV radiation sensitivity. This sensitizing effect of ΔdinB883 is assumed to be completely dependent upon the presence of UmuDC protein; since the ΔdinB883 mutation did not sensitize the ΔumuDC strain to UV radiation killing throughout log phase and early stationary phase growth. The DNA damage checkpoint activity of UmuDC was clearly affected by ΔdinB883 as shown by testing a umuC104 ΔdinB883 double-mutant. The sensitivities of the ΔumuDC strain and the ΔdinB883 ΔumuDC double-mutant strain were significantly greater than for the ΔdinB883 strain, suggesting that the ΔdinB883 allele only partially suppresses UmuDC activity. The ΔdinB883 mutation partially sensitized (fivefold) uvrA and uvr

  2. A flavonoid mutant of barley (Hordeum vulgare L.) exhibits increased sensitivity to UV-B radiation in the primary leaf

    International Nuclear Information System (INIS)

    Reuber, S.; Bornman, J.F.; Weissenböck, G.

    1996-01-01

    The aim of the present investigation was to define the role of soluble flavonoids as UV-B protectants in the primary leaf of barley (Hordeum vulgare L.). For this purpose we used a mutant line (Ant 287) from the Carlsberg collection of proanthocyanidin-free barley containing only 7% of total extractable flavonoids in the primary leaf as compared to the mother variety (Hiege 550/75). Seven-day-old leaves from plants grown under high visible light with or without supplementary UV-B radiation were used for the determination of UV-B sensitivity. UV-B-induced changes were assessed from parameters of chlorophyll fluorescence of photosystem II, including initial and maximum fluorescence, apparent quantum yield, and photochemical and non-photochemical quenching. A quartz fibre-optic microprobe was used to evaluate the amount of potentially harmful UV-B (310 nm radiation) penetrating into the leaf as a direct consequence of flavonoid deficiency. Our data indicate an essential role of flavonoids in UV-B protection of barley primary leaves. In leaves of the mutant line grown under supplementary UV-B, an increase in 310nm radiation in the mesophyll and a strong decrease in the quantum yield of photosynthesis were observed as compared to the corresponding mother variety. Primary leaves of liege responded to supplementary UV-B radiation with a 30% increase in the major flavonoid saponarin and a 500% increase in the minor compound lutonarin. This is assumed to be an efficient protective response since no changes in variable chlorophyll fluorescence were apparent. In addition, a further reduction in UV-B penetration into the mesophyll was recorded in these leaves

  3. Bactericidal activity under UV and visible light of cotton fabrics coated with anthraquinone-sensitized TiO2

    KAUST Repository

    Rahal, Raed; Le Bé chec, Mickaë l; Guyoneaud, Ré my; Pigot, Thierry; Paolacci, H.; Lacombe, Sylvie M.

    2013-01-01

    . The experimental method allowed the accurate quantification of bacteria survival on photoactive surfaces and films under UVA and UV-free visible irradiation. Cotton fabrics coated with TiO2, anthraquinone or anthraquinone-sensitized TiO2 display a significant

  4. Sensitivity of strains of Escherichia coli differing in repair capability to far UV, near UV and visible radiations

    International Nuclear Information System (INIS)

    Webb, R.B.; Brown, M.S.

    1976-01-01

    In stationary phase, strains of Escherichia coli deficient in excision (B/r Hcr) or recombination repair (K12 AB2463) were more sensitive than a repair proficient strain (B/r) to monochromatic near-ultraviolet (365nm) and visible (460 nm) radiations. The relative increase in sensitivity of mutants deficient in excision or recombination repair in comparison to the wildtype, was less at 365 nm than at 254 nm. However, a strain deficient in both excision and recombination repair (K12 AB2480) showed a large, almost equal, increase in sensitivity over mutants deficient in either excision or recombination repair at 365 nm and 254 nm. All strains tested were highly resistant to 650 nm radiation. Action spectra for lethality of strains B/r and B/r Hcr in stationary phase reveal small peaks or shoulders in the 330 to 340, 400 to 410 and 490 to 510 nm wavelength ranges. The presence of 5 micro g/ml acriflavine (an inhibitor of repair) in the plating medium greatly increased the sensitivity of strain B/r to radiation at 254, 365 and 460 nm, while strains E.coli B/r Hcr and K12 AB2463 were sensitized by small amounts. At each of the wavelengths tested, acriflavine in the plating medium had at most a small effect on E.coli K12 AB2480. Acriflavine failed to sensitize any strain tested at 650 nm. Evidence supports the interpretation that lesions induced in DNA by 365 nm and 460 nm radiations play the major role in the inactivation of E.coli by these wavelengths. Single-strand breaks (or alkali-labile bonds), but not pyrimidine dimers are candidates for the lethal DNA lesions in uvrA and repair proficient strains. At high fluences lethality may be enhanced by damage to the excision and recombination repair systems. (author)

  5. Sensitivity of strains of Escherichia coli differing in repair capability to far uv, near uv and visible radiations

    Energy Technology Data Exchange (ETDEWEB)

    Webb, R B; Brown, M S [Argonne National Lab., Ill. (USA)

    1976-11-01

    In stationary phase, strains of Escherichia coli deficient in excision (B/r Hcr) or recombination repair (K12 AB2463) were more sensitive than a repair proficient strain (B/r) to monochromatic near-ultraviolet (365nm) and visible (460 nm) radiations. The relative increase in sensitivity of mutants deficient in excision or recombination repair in comparison to the wildtype, was less at 365 nm than at 254 nm. However, a strain deficient in both excision and recombination repair (K12 AB2480) showed a large, almost equal, increase in sensitivity over mutants deficient in either excision or recombination repair at 365 nm and 254 nm. All strains tested were highly resistant to 650 nm radiation. Action spectra for lethality of strains B/r and B/r Hcr in stationary phase reveal small peaks or shoulders in the 330 to 340, 400 to 410 and 490 to 510 nm wavelength ranges. The presence of 5 micro g/ml acriflavine (an inhibitor of repair) in the plating medium greatly increased the sensitivity of strain B/r to radiation at 254, 365 and 460 nm, while strains E.coli B/r Hcr and K12 AB2463 were sensitized by small amounts. At each of the wavelengths tested, acriflavine in the plating medium had at most a small effect on E.coli K12 AB2480. Acriflavine failed to sensitize any strain tested at 650 nm. Evidence supports the interpretation that lesions induced in DNA by 365 nm and 460 nm radiations play the major role in the inactivation of E.coli by these wavelengths. Single-strand breaks (or alkali-labile bonds), but not pyrimidine dimers are candidates for the lethal DNA lesions in uvrA and repair proficient strains. At high fluences lethality may be enhanced by damage to the excision and recombination repair systems.

  6. Genetic control of near-UV (300-400 nm) sensitivity independent of the recA gene in strains of Escherichia coli K12

    International Nuclear Information System (INIS)

    Tuveson, R.W.; Jonas, R.B.

    1979-01-01

    Stationary cells of isogenic pairs of Escherichia coli K12 strains presumably differing only in the recA function, were inactivated with near-UV (300-400 nm) radiation. Based on near-UV inactivation kinetics, the strains can be divided into two discrete categories in which near-UV sensitivity does not necessarily correlate with far-UV sensitivity conferred by two different recA alleles. Lack of overlap between near-UV and far-UV (recA) sensitivity can be explained by assuming that a different chromosomal gene (nur) controls near-UV sensitivity. Support for this hypothesis came from a mating experiment in which four selected recombinants, isogenic with respect to auxotrophic markers, were identified exhibiting all four possible combinations of far-UV (recA1 vs recA + ) and near-UV sensitivity (nur vs nur + ). Transduction with phase P1 showed that introduction of the recA1 allele into a recA + recipient did not affect the near-UV sensitivity of the recipient. Additional matings together with transduction experiments suggested that the nur gene is located at a position on the E. coli linkage map clearly separable from recA (minute 58). (author)

  7. The EGFR family of receptors sensitizes cancer cells towards UV light

    DEFF Research Database (Denmark)

    Petersen, Steffen B.; Neves Petersen, Teresa; Olsen, Birgitte

    2008-01-01

    A combination of bioinformatics, biophysical, advanced laser studies and cell biology lead to the realization that laser-pulsed UV light stops cancer growth and induces apoptosis. We have previously shown that laser-pulsed UV (LP-UV) illumination of two different skin-derived cancer cell lines both...... bridges. The EGF receptor is often overexpressed in cancers and other proliferative skin disorders, it might be possible to significantly reduce the proliferative potential of these cells making them good targets for laser-pulsed UV-light treatment. The discovery that UV light can be used to open...... disulphide bridges in proteins upon illumination of nearby aromatic amino acids was the first step that lead to the hypothesis that UV light could modulate the structure and therefore the function of these key receptor proteins. The observation that membrane receptors (EGFR) contained exactly the motifs...

  8. Responses of phylloplane yeasts to UV-B (290-320 nm) radiation: interspecific differences in sensitivity

    International Nuclear Information System (INIS)

    Gunasekera, T.S.; Paul, N.D.; Ayres, P.G.

    1997-01-01

    The sensitivity to UV-B (290–320 nm) radiation of common phylloplane yeasts from two contrasting UV-B environments was compared in the laboratory using mixtures of white light (PAR: 400–700 nm) and UV-B radiation from artificial lamp sources. Sporidiobolus salmonicolor, Rhodotorula mucilaginosa and Cryptococcus sp., the dominant yeasts on leaves of tea (Camellia sinensis), were isolated in Sri Lanka (SL), while Sporidiobolus sp. and Bullera alba, dominant on faba bean (Vicia faba), were isolated in the U.K. Dose responses were determined separately for each yeast. UV-B reduced colony forming units (due to cell mortality or inactivation) and colony size (due to reduced multiplication) of all yeasts. The LD 50 values and doses causing 50% reduction of cells per colony were higher for SL isolates than U.K. isolates. Results indicated that each yeast is somewhat vulnerable to UV-B doses representative of its natural habitat. The relative insensitivity of SL isolates was shown when SL and U.K. isolates were irradiated simultaneously with the same dose of UV-B. Of the two U.K. yeasts, B. alba was significantly more sensitive than Sporidiobolus sp. to UV-B. Except for R. mucilaginosa from SL, all yeasts demonstrated some photorepair in the presence of white light. White light provided relatively little protection for the U.K. isolate of Sporidiobolus sp. although it allowed increased colony size. The spectral responses of Sporidiobolus sp. (U.K.) and of B. alba (U.K.) were broadly similar. Wavelengths longer than 320 nm had no measurable effect on colony forming units. However, colony survival was significantly reduced at 310 nm and all shorter wavebands. No colonies were counted at 290 nm or below. (author)

  9. Interspecies complementation analysis of xeroderma pigmentosum and UV-sensitive Chinese hamster cells

    International Nuclear Information System (INIS)

    Stefanini, M.; Keijzer, W.; Westerveld, A.; Bootsma, D.

    1985-01-01

    Complementation analysis was performed 24 h after fusion of UV-sensitive CHO cells (CHO 12 RO) with XP cells of complementation groups A, B, C, D, F and G. The parental cells are characterized by low levels of unscheduled DNA synthesis (UDS). In all combinations, the UDS levels observed in heterokaryons were higher than those in parental mutant cells, clearly indicating cooperation of human and Chinese hamster repair functions. In heterokaryons of CHO 12 RO with XP-A and XP-C cells, the UDS values reached about the normal human level, whereas in heterokaryons with XP-B, XP-D and XP-F, UDS was restored at a level approaching that in wild-type CHO cells. The results obtained after fusion of CHO cells with two representative cell strains from the XP-G group, XP 2 BI and XP 3 BR, were inconsistent. Fusion with XP 3 BR cells yielded UDS levels ranging from wild-type Chinese hamster to normal human, whereas fusion with XP 2 BI cells resulted in a slight increase in UDS which even after 48 h remained below the level found in wild-type CHO cells. The occurrence of complementation in these interspecies heterokaryons indicates that the genetic defect in the CHO 12 RO cells is different from the defects in the XP complementation groups tested

  10. Properties of a novel radiophotoluminescent readout system using a cw modulated UV laser diode and phase-sensitive technique

    International Nuclear Information System (INIS)

    Zhao, C.; Kurobori, T.; Miyamoto, Y.; Yamamoto, T.

    2011-01-01

    We have proposed and constructed a novel readout system for measuring a dose-dependent radiophotoluminescence (RPL) signal of a silver-activated phosphate glass dosimeter. The present reader consists of a modulated continuous-wave (cw) ultraviolet (UV) laser diode at 375 nm as an excitation and a phase-sensitive technique using a lock-in amplifier. Preliminary results using a home-made reader are compared with those of the conventional technique based on a combination of a pulsed UV N 2 laser excitation at 337 nm and a photon counting system.

  11. Glass sealing

    Energy Technology Data Exchange (ETDEWEB)

    Brow, R.K.; Kovacic, L.; Chambers, R.S. [Sandia National Labs., Albuquerque, NM (United States)

    1996-04-01

    Hernetic glass sealing technologies developed for weapons component applications can be utilized for the design and manufacture of fuel cells. Design and processing of of a seal are optimized through an integrated approach based on glass composition research, finite element analysis, and sealing process definition. Glass sealing procedures are selected to accommodate the limits imposed by glass composition and predicted calculations.

  12. Inhibition of charge recombination for enhanced dye-sensitized solar cells and self-powered UV sensors by surface modification

    Energy Technology Data Exchange (ETDEWEB)

    Chu, Liang, E-mail: chuliang@njupt.edu.cn [Advanced Energy Technology Center, Nanjing University of Posts and Telecommunications (NUPT), Nanjing 210046 (China); Wuhan National Laboratory for Optoelectronics (WNLO)-School of Physics, Huazhong University of Science and Technology (HUST), Wuhan 430074 (China); Qin, Zhengfei; Liu, Wei [School of Materials Science and Engineering (SMSE), Nanjing University of Posts and Telecommunications (NUPT), Nanjing 210046 (China); Ma, Xin’guo, E-mail: maxg2013@sohu.com [Hubei Collaborative Innovation Center for High-efficiency Utilization of Solar Energy, Hubei University of Technology, Wuhan 430068 (China)

    2016-12-15

    Graphical abstract: Inhibition of charge recombination was utilized to prolong electrode lifetime in dye-sensitized solar cells (DSSCs) and self-powered UV sensors based on TiO{sub 2}-modified SnO{sub 2} photoelectrodes. The electrochemical impedance spectroscopy and open-circuit voltage decay measurements indicated that the electron lifetime was significantly prolonged in DSSCs after TiO{sub 2} modification. And in self-powered UV sensors, the sensitivity and response time were enhanced. - Highlights: • The surface modification to inhibit charge recombination was utilized in photovoltaic devices. • Inhibition of charge recombination can prolong electrode lifetime in photovoltaic devices. • Enhanced DSSCs and self-powered UV sensors based on SnO{sub 2} photoelectrodes were obtained by TiO{sub 2} modification. - Abstract: The surface modification to inhibit charge recombination was utilized in dye-sensitized solar cells (DSSCs) and self-powered ultraviolet (UV) sensors based on SnO{sub 2} hierarchical microspheres by TiO{sub 2} modification. For DSSCs with SnO{sub 2} photoelectrodes modified by TiO{sub 2}, the power conversion efficiency (PCE) was improved from 1.40% to 4.15% under standard AM 1.5G illumination (100 mW/cm{sup 2}). The electrochemical impedance spectroscopy and open-circuit voltage decay measurements indicated that the charge recombination was effectively inhibited, resulting in long electron lifetime. For UV sensors with SnO{sub 2} photoelectrodes modified by TiO{sub 2} layer, the self-powered property was more obvious, and the sensitivity and response time were enhanced from 91 to 6229 and 0.15 s to 0.055 s, respectively. The surface modification can engineer the interface energy to inhibit charge recombination, which is a desirable approach to improve the performance of photoelectric nanodevice.

  13. Sealing device

    Science.gov (United States)

    Garcia-Crespo, Andres Jose

    2013-12-10

    A sealing device for sealing a gap between a dovetail of a bucket assembly and a rotor wheel is disclosed. The sealing device includes a cover plate configured to cover the gap and a retention member protruding from the cover plate and configured to engage the dovetail. The sealing device provides a seal against the gap when the bucket assemply is subjected to a centrifugal force.

  14. Photoproduct formation and repair capacity in a mutant of Bacillus cereus 569 producing UV-sensitive spores

    International Nuclear Information System (INIS)

    Weinberger, S.; Evenchik, Z.; Hertman, I.; Bar-Ilan Univ., Ramat-Gan

    1982-01-01

    A mutant of Bacillus cereus 569 UV sensitive in both vegetative and sporal stages was isolated by N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis followed by selection on mitomycin C. The UV-sensitive mutant designated as B. cereus 2422 exhibited normal content of dipicolinic acid (DPA) and resistance to X-rays and ethyl methanesulphonate. The photoproduct type and amount, induced by a given UV dose, was similar in either cells or spores of both the mutant 2422 and the wild-type ancestor. The mutant 2422 excised cyclobutane thymine dimers only to a limited extent (20%) as compared with 80% removal in the wild type. Removal of a spore-specific photoproduct (TDHT) during germination proceeded to a similar extent in B. cereus 2422 and the wild-type parent. However, under growing conditions, an additional removal of the TDHT was observed only in the wild-type strain. Liquid holding recovery occurred in irradiated wild-type cells, but not in mutant cells. Spontaneous revertants were isolated that regained UV resistance simultaneously in both the vegetative and sporal stage. (orig./AJ)

  15. Effect of 5-bromouracil and 5-bromo-2-deoxyuridine in combination with 8-azaadenine on UV sensitivity of bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Jacob, H.E. (Akademie der Wissenschaften der DDR, Jena. Zentralinstitut fuer Mikrobiologie und Experimentelle Therapie); Golovinsky, E. (Bylgarska Akademiya na Naukite, Sofia)

    1983-01-01

    The presence of 5-bromouracil (BU) as well as 5-bromo-2-deoxyuridine (BUdR) in the cultivation media of bacteria results in the distinct increase of UV sensitivity. With the nucleic acid base analogue 8-azaadenine (8-AA) a similar effect was confirmed, however, not so pronounced. The combined action of BU or BUdR and 8-AA on Escherichia coli, Proteus mirabilis, Bacillus subtilis and Bacillus cereus was investigated. The sensitization effect of BUdR does not increase if 8-AA is present additionally during cultivation. On the contrary, a decrease of sensibilization occurs. This may be caused by the protective effect of the adenine derivative against UV irradiation, if it is present in the cell, but not incorporated into the DNA.

  16. Effect of 5-bromouracil and 5-bromo-2-deoxyuridine in combination with 8-azaadenine on UV sensitivity of bacteria

    International Nuclear Information System (INIS)

    Jacob, H.E.; Golovinsky, E.

    1983-01-01

    The presence of 5-bromouracil (BU) as well as 5-bromo-2-deoxyuridine (BUdR) in the cultivation media of bacteria results in the distinct increase of UV sensitivity. With the nucleic acid base analogue 8-azaadenine (8-AA) a similar effect was confirmed, however, not so pronounced. The combined action of BU or BUdR and 8-AA on Escherichia coli, Proteus mirabilis, Bacillus subtilis and Bacillus cereus was investigated. The sensitization effect of BUdR does not increase if 8-AA is present additionally during cultivation. On the contrary, a decrease of sensibilization occurs. This may be caused by the protective effect of the adenine derivative against UV irradiation, if it is present in the cell, but not incorporated into the DNA. (author)

  17. Mechanical seals

    CERN Document Server

    Mayer, E

    1977-01-01

    Mechanical Seals, Third Edition is a source of practical information on the design and use of mechanical seals. Topics range from design fundamentals and test rigs to leakage, wear, friction and power, reliability, and special designs. This text is comprised of nine chapters; the first of which gives a general overview of seals, including various types of seals and their applications. Attention then turns to the fundamentals of seal design, with emphasis on six requirements that must be considered: sealing effectiveness, length of life, reliability, power consumption, space requirements, and c

  18. Free-swimming northern elephant seals have low field metabolic rates that are sensitive to an increased cost of transport

    DEFF Research Database (Denmark)

    Maresh, Jennifer L; Simmons, Samantha E; Crocker, Daniel E

    2014-01-01

    locomotion, elephant seals had low energy requirements (106.5±28.2 kJ kg−1 day−1), approaching or even falling below predictions of basal requirements. Seals responded to a small increase in locomotion costs by spending more time resting between dives (149±44 s) compared with matched control treatments (102......Widely ranging marine predators often adopt stereotyped, energy-saving behaviours to minimize the energetic cost of transport while maximizing energy gain. Environmental and anthropogenic disturbances can disrupt energy balance by prompting avoidance behaviours that increase transport costs...

  19. Sensitivity of two ecotypes of Arabidopsis Thaliana (Cvi and Te) towards UV-B irradiation

    International Nuclear Information System (INIS)

    Velichkova, M.; Stanoeva, D.; Popova, A.

    2013-01-01

    he susceptibility of Arabidopsis thaliana towards the detrimental effect of UV-B irradiation was investigated using two ecotypes, Cvi and Te. The effect of UV-B treatment on primary photosynthetic reactions - energy interaction between the main pigment-protein complexes and oxygen evolution, was evaluated at low (4 0 C) and at room (22 0 C) temperature. UV-B-induced alterations of investigated photosynthetic reactions are better expressed at 22 0 C than at 4 0 C for Cvi. For Te ecotype the energy interaction was suppressed to higher extent at 22 0 C, while oxygen evolving activity was affected similarly at both temperatures. At low and room temperature, the energy interaction in the complex PSII-core antenna is affected stronger by UV-B treatment than the energy distribution between both photosystems, as revealed by fluorescence ratios of 77 K spectra. The results presented indicate that the Arabidopsis thaliana ecotype Cvi (Cape Verde Islands) is less affected by UV-B irradiation in respect to the investigated primary photosynthetic reactions than the ecotype Te (Finland)

  20. Security seal

    Science.gov (United States)

    Gobeli, Garth W.

    1985-01-01

    Security for a package or verifying seal in plastic material is provided by a print seal with unique thermally produced imprints in the plastic. If tampering is attempted, the material is irreparably damaged and thus detectable. The pattern of the imprints, similar to "fingerprints" are recorded as a positive identification for the seal, and corresponding recordings made to allow comparison. The integrity of the seal is proved by the comparison of imprint identification records made by laser beam projection.

  1. Seal arrangement

    International Nuclear Information System (INIS)

    Dempsey, J.D.

    1978-01-01

    A hydraulically balanced face type shaft seal is provided in which the opening and closing seal face areas retain concentricity with each other in the event of lateral shaft displacement. The seal arrangement is for a vertical high pressure pump, indented for use in the cooling system of a nuclear reactor. (Auth.)

  2. Association of mutator activity with UV sensitivity in an aphidicolin-resistant mutant of Chinese hamster V79 cells

    International Nuclear Information System (INIS)

    Liu, P.K.; Chang, C.; Trosko, J.E.

    1982-01-01

    The spontaneous mutation rates of an ultraviolet light (UV)-sensitive aphidicolin-resistant mutant (aphsup(r)-4-2) and its revertants have been determined by 2 techniques. By using the fluctuation analysis, the mutant and its thymidine (TdR)-prototrophic 'revertant' were found to exhibit elevated spontaneous mutation rates at the 6-thioguanine- and diphtheria-toxin-resistant loci. In contrast, the TdR-auxotrophic 'revertant' did not show this property. Similar results were obtained by the multiple replating technique. From these comparative studies and other previous characterizations, it appears that a single gene mutation is responsible for the following pleiotropic phenotype: slow growth, UV sensitivity, high UV-induced mutability, high frequency of site-specific bromodeoxyuridine (BrdU)-dependent chromosome breaks and enhanced spontaneous mutation rate. Recent studies indicate that the mutation may be on the gene for DNA polymerase α. The results further indicate that thymidine auxotrophy or imbalance in nucleotide pools is not necessarily associated with the mutator activity in mammalian cells. (orig.)

  3. Sensitivity of upper atmospheric emissions calculations to solar/stellar UV flux

    Directory of Open Access Journals (Sweden)

    Barthelemy Mathieu

    2014-01-01

    Full Text Available The solar UV (UltraViolet flux, especially the EUV (Extreme UltraViolet and FUV (Far UltraViolet components, is one of the main energetic inputs for planetary upper atmospheres. It drives various processes such as ionization, or dissociation which give rise to upper atmospheric emissions, especially in the UV and visible. These emissions are one of the main ways to investigate the upper atmospheres of planets. However, the uncertainties in the flux measurement or modeling can lead to biased estimates of fundamental atmospheric parameters, such as concentrations or temperatures in the atmospheres. We explore the various problems that can be identified regarding the uncertainties in solar/stellar UV flux by considering three examples. The worst case appears when the solar reflection component is dominant in the recorded spectrum as is seen for outer solar system measurements from HST (Hubble Space Telescope. We also show that the estimation of some particular line parameters (intensity and shape, especially Lyman α, is crucial, and that both total intensity and line profile are useful. In the case of exoplanets, the problem is quite critical since the UV flux of their parent stars is often very poorly known.

  4. Structural characteristics and UV-light enhanced gas sensitivity of La-doped ZnO nanoparticles

    International Nuclear Information System (INIS)

    Ge Chunqiao; Xie Changsheng; Hu Mulin; Gui Yanghai; Bai Zikui; Zeng Dawen

    2007-01-01

    La-doped ZnO nanoparticles were synthesized by sol-gel method starting from zinc acetate dihydrate, lanthanum sesquioxide, alcohol and nitric acid. The crystal structure and morphology of the nanoparticles were characterized by XRD, FESEM, respectively. The thermal decomposition behavior of the the ZnO-based xerogel was detected by TG-DSC. The results show that as-prepared nanoparticles with the hexagonal wurtzite contain the adsorbed water and some organic compounds below 300 o C, which is the key to the calcinations of the ZnO-based xerogel. Pure ZnO and La-doped ZnO thick film sensors were prepared and tested for specific sensitivity to alcohol and benzene with (and without) UV-light excitation. Among all, 10 at.%La-ZnO-based sensors are significantly sensitive to 100 ppm alcohol and 100 ppm benzene. There is an obvious enhancement of the gas-sensing performances with UV-light excitation. That is, the sensitivity to 100 ppm benzene rises twice. The observed sensitivity to alcohol and benzene could be explained with the surface adsorption theory and the conduction-band theory

  5. Fabrication and characterization of UV-emitting nanoparticles as novel radiation sensitizers targeting hypoxic tumor cells

    Science.gov (United States)

    Squillante, Michael R.; Jüstel, Thomas; Anderson, R. Rox; Brecher, Charles; Chartier, Daniel; Christian, James F.; Cicchetti, Nicholas; Espinoza, Sara; McAdams, Daniel R.; Müller, Matthias; Tornifoglio, Brooke; Wang, Yimin; Purschke, Martin

    2018-06-01

    Radiation therapy is one of the primary therapeutic techniques for treating cancer, administered to nearly two-thirds of all cancer patients. Although largely effective in killing cancer cells, radiation therapy, like other forms of cancer treatment, has difficulty dealing with hypoxic regions within solid tumors. The incomplete killing of cancer cells can lead to recurrence and relapse. The research presented here is investigating the enhancement of the efficacy of radiation therapy by using scintillating nanoparticles that emit UV photons. UV photons, with wavelengths between 230 nm and 280 nm, are able to inactivate cells due to their direct interaction with DNA, causing a variety of forms of damage. UV-emitting nanoparticles will enhance the treatment in two ways: first by generating UV photons in the immediate vicinity of cancer cells, leading to direct and oxygen-independent DNA damage, and second by down-converting the applied higher energy X-rays into softer X-rays and particles that are more efficiently absorbed in the targeted tumor region. The end result will be nanoparticles with a higher efficacy in the treatment of hypoxic cells in the tumor, filling an important, unmet clinical need. Our preliminary experiments show an increase in cell death using scintillating LuPO4:Pr nanoparticles over that achieved by the primary radiation alone. This work describes the fabrication of the nanoparticles, their physical characterization, and the spectroscopic characterization of the UV emission. The work also presents in vitro results that demonstrate an enhanced efficacy of cell killing with x-rays and a low unspecific toxicity of the nanoparticles.

  6. Printable UV personal dosimeter: sensitivity as a function of DoD parameters and number of layers of a functional photonic ink

    Science.gov (United States)

    Sousa, Felipe L. N.; Mojica-Sánchez, Lizeth C.; Gavazza, Sávia; Florencio, Lourdinha; Vaz, Elaine C. R.; Santa-Cruz, Petrus A.

    2016-04-01

    This work presents ‘intelligent papers’ obtained by functional inks printed on cellulose-sheets by DoD inkjet technology and their performance as a photonic device for UV-radiation dosimetry. The dosimeter operation is based on the photodegradation of the active part of a photonic ink, btfa (4,4,4-trifluoro-1-phenyl-1,3-butanedione) ligands in Eu(III) complex, as a function of the UV dose (Jcm-2), and the one-way device is read by the luminescence quenching of (5D0 → 7F2) Eu3+ transition after UV exposure of the printed paper. The printed dosimeter presented an exponential behavior, measured here up to 10 Jcm-2 for UV-A, UV-B and UV-C, and it was shown that the number of jetted layers could fit the dosimeter sensitivity.

  7. Printable UV personal dosimeter: sensitivity as a function of DoD parameters and number of layers of a functional photonic ink

    International Nuclear Information System (INIS)

    Sousa, Felipe L N; Mojica-Sánchez, Lizeth C; Vaz, Elaine C R; Santa-Cruz, Petrus A; Gavazza, Sávia; Florencio, Lourdinha

    2016-01-01

    This work presents ‘intelligent papers’ obtained by functional inks printed on cellulose-sheets by DoD inkjet technology and their performance as a photonic device for UV-radiation dosimetry. The dosimeter operation is based on the photodegradation of the active part of a photonic ink, btfa (4,4,4-trifluoro-1-phenyl-1,3-butanedione) ligands in Eu(III) complex, as a function of the UV dose (Jcm −2 ), and the one-way device is read by the luminescence quenching of ( 5 D 0  →  7 F 2 ) Eu 3+ transition after UV exposure of the printed paper. The printed dosimeter presented an exponential behavior, measured here up to 10 Jcm −2 for UV-A, UV-B and UV-C, and it was shown that the number of jetted layers could fit the dosimeter sensitivity. (paper)

  8. Complementation of the UV-sensitive phenotype of a xeroderma pigmentosum human cell line by transfection with a cDNA clone library

    International Nuclear Information System (INIS)

    Teitz, T.; Naiman, T.; Avissar, S.S.; Bar, S.; Okayama, H.; Canaani, D.

    1987-01-01

    In previous work, a xeroderma pigmentosum cell line belonging to complementation group C was established by transformation with origin-defective simian virus 40. We now report the complementation of the UV sensitivity of this cell line by gene transfer. A human cDNA clone library constructed in a mammalian expression vector, and itself incorporated in a lambda phage vector, was introduced into the cells as a calcium phosphate precipitate. Following selection to G418 resistance, provided by the neo gene of the vector, transformants were selected for UV resistance. Twenty-one cell clones were obtained with UV-resistance levels typical of normal human fibroblasts. All transformants contained vector DNA sequences in their nuclei. Upon further propagation in the absence of selection for G418 resistance, about half of the primary transformants remained UV-resistant. Secondary transformants were generated by transfection with a partial digest of total chromosomal DNA from one of these stable transformants. This resulted in 15 G418-resistant clones, 2 of which exhibited a UV-resistant phenotype. The other primary clones lost UV resistance rapidly when subcultured in the absence of G418. Importantly, several retained UV resistance under G418 selection pressure. The acquisition of UV resistance by secondary transformants derived by transfection of DNA from a stable primary transformant, and the linkage between G418 and UV resistances in the unstable primary transformants, strongly suggests that the transformants acquired UV resistance through DNA-mediated gene transfer and not by reversion

  9. Epidermal transmittance and phenolic composition in leaves of atrazine-tolerant and atrazine-sensitive cultivars of Brassica napus grown under enhanced UV-B radiation

    International Nuclear Information System (INIS)

    Olsson, L.C.; Veit, M.; Bornman, J.F.

    1999-01-01

    Experiments were conducted on the atrazine-tolerant mutant Stallion and the atrazine-sensitive cv. Paroll of Brassica napus L., which were grown under either visible light or with the addition of UV-B radiation (280–320 nm) for 15 days. The mutant has been shown to be sensitive to high levels of visible light as compared to the atrazine-sensitive cultivar and therefore we wished to determine plant response to UV-B radiation with respect to potential pigment changes, certain anatomical features, radiation penetration and partial photosynthesis. With regard to pigment changes, we were particularly interested in whether the compositional shift in flavonol pigments under enhanced UV-B radiation, previously suggested to favour increased antioxidant activity, is confined to the adaxial epidermis, which generally receives most UV-B radiation or whether the pigment shift is also inducible in the abaxial epidermis.As was to be expected, the penetration of UV-B radiation (310 nm) was lower in the UV-B-exposed plants, which was correlated with an increased amount of UV-screening pigments in the adaxial and abaxial epidermal layers. The main flavonoid glycosides showed the largest shift from kaempferol to quercetin as aglycone moiety in the adaxial epidermal layer. However, in the abaxial epidermal layer the hydroxycinnamic acid (HCA) derivatives and kaempferol glycosides were predominant. Penetration of 430 nm light was higher after UV-B exposure, and probably contributed to the fact that photosynthetic efficiency of photosystem II was unchanged or higher after UV-B exposure. UV-B radiation decreased leaf area in the atrazine-tolerant mutant only. Both cultivars showed an increased leaf thickness after UV-B exposure due to cell elongation mainly of the palisade tissue. This was especially evident in the mutant

  10. Improved analysis of Monascus pigments based on their pH-sensitive UV-Vis absorption and reactivity properties.

    Science.gov (United States)

    Shi, Kan; Chen, Gong; Pistolozzi, Marco; Xia, Fenggeng; Wu, Zhenqiang

    2016-09-01

    Monascus pigments, a mixture of azaphilones mainly composed of red, orange and yellow pigments, are usually prepared in aqueous ethanol and analysed by ultraviolet-visible (UV-Vis) spectroscopy. The pH of aqueous ethanol used during sample preparation and analysis has never been considered a key parameter to control; however, this study shows that the UV-Vis spectra and colour characteristics of the six major pigments are strongly influenced by the pH of the solvent employed. In addition, the increase of solvent pH results in a remarkable increase of the amination reaction of orange pigments with amino compounds, and at higher pH (≥ 6.0) a significant amount of orange pigment derivatives rapidly form. The consequent impact of these pH-sensitive properties on pigment analysis is further discussed. Based on the presented results, we propose that the sample preparation and analysis of Monascus pigments should be uniformly performed at low pH (≤ 2.5) to avoid variations of UV-Vis spectra and the creation of artefacts due to the occurrence of amination reactions, and ensure an accurate analysis that truly reflects pigment characteristics in the samples.

  11. Nozzle seal

    International Nuclear Information System (INIS)

    Herman, R.F.

    1977-01-01

    In an illustrative embodiment of the invention, a nuclear reactor pressure vessel, having an internal hoop from which the heated coolant emerges from the reactor core and passes through to the reactor outlet nozzles, is provided with sealing members operatively disposed between the outlet nozzle and the hoop. The sealing members are biased against the pressure vessel and the hoop and are connected by a leak restraining member establishing a leak-proof condition between the inlet and outlet coolants in the region about the outlet nozzle. Furthermore, the flexible responsiveness of the seal assures that the seal will not structurally couple the hoop to the pressure vessel

  12. Nozzle seal

    International Nuclear Information System (INIS)

    Walling, G.A.

    1977-01-01

    In an illustrative embodiment of the invention, a nuclear reactor pressure vessel, having an internal hoop from which the heated coolant emerges from the reactor core and passes through to the reactor outlet nozzles, is provided with sealing rings operatively disposed between the outlet nozzles and the hoop. The sealing rings connected by flexible members are biased against the pressure vessel and the hoop, establishing a leak-proof condition between the inlet and outlet coolants in the region about the outlet nozzle. Furthermore, the flexible responsiveness of the seal assures that the seal will not structurally couple the hoop to the pressure vessel. 4 claims, 2 figures

  13. Fast and Sensitive Solution-Processed Visible-Blind Perovskite UV Photodetectors.

    Science.gov (United States)

    Adinolfi, Valerio; Ouellette, Olivier; Saidaminov, Makhsud I; Walters, Grant; Abdelhady, Ahmed L; Bakr, Osman M; Sargent, Edward H

    2016-09-01

    The first visible-blind UV photodetector based on MAPbCl3 integrated on a substrate exhibits excellent performance, with responsivities reaching 18 A W(-1) below 400 nm and imaging-compatible response times of 1 ms. This is achieved by using substrate-integrated single crystals, thus overcoming the severe limitations affecting thin films and offering a new application of efficient, solution-processed, visible-transparent perovskite optoelectronics. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. Sensitivity of bacteria to photoactivated titanium dioxide in comparison with UV irradiation

    International Nuclear Information System (INIS)

    Kersters, Ilse; De Keyser, Tilly; Verstraete, Willy

    1998-01-01

    Titanium dioxide was used as a photocatalyst to generate hydroxyl radicals in a flowthrough water reactor. Experiments were performed with cultures of Aeromonas hydrophila AWWX1 and Pseudomonas fluorescens R 2 f to evaluate the disinfection capabilities of the reactor. Although a decrease in viable counts was observed with long-wavelength (λ=370 nm) irradiated TiO 2 pellets, direct UV 254 irradiation seems a superior technology for the disinfection of transparent potable water since the viable counts of the test strains declined stronger (2-5 logs) and faster (20x) in UV 254 -treated water than in photoactivated TiO 2 -treated water. Outdoor tests conducted in the summer noonday sun showed that the viable counts of Aeromonas hydrophila AWWX1 decreased strongly (ca 5 log units) in transparent and turbid water samples (750 NTU) exposed to natural sunlight (47,000 lux). The addition of TiO 2 to the solar irradiated waters did not influence the die-off of the strain. These observations indicate that the photocatalytic approach does not offer real prospects as an alternative technology for the disinfection of drinking water. (author)

  15. Ceramic Seal.

    Energy Technology Data Exchange (ETDEWEB)

    Smartt, Heidi A. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Romero, Juan A. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Custer, Joyce Olsen [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Hymel, Ross W. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Krementz, Dan [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Gobin, Derek [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Harpring, Larry [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Martinez-Rodriguez, Michael [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Varble, Don [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); DiMaio, Jeff [Tetramer Technologies, Pendleton, SC (United States); Hudson, Stephen [Tetramer Technologies, Pendleton, SC (United States)

    2016-11-01

    Containment/Surveillance (C/S) measures are critical to any verification regime in order to maintain Continuity of Knowledge (CoK). The Ceramic Seal project is research into the next generation technologies to advance C/S, in particular improving security and efficiency. The Ceramic Seal is a small form factor loop seal with improved tamper-indication including a frangible seal body, tamper planes, external coatings, and electronic monitoring of the seal body integrity. It improves efficiency through a self-securing wire and in-situ verification with a handheld reader. Sandia National Laboratories (SNL) and Savannah River National Laboratory (SRNL), under sponsorship from the U.S. National Nuclear Security Administration (NNSA) Office of Defense Nuclear Nonproliferation Research and Development (DNN R&D), have previously designed and have now fabricated and tested Ceramic Seals. Tests have occurred at both SNL and SRNL, with different types of tests occurring at each facility. This interim report will describe the Ceramic Seal prototype, the design and development of a handheld standalone reader and an interface to a data acquisition system, fabrication of the seals, and results of initial testing.

  16. Ceramic Seal

    International Nuclear Information System (INIS)

    Smartt, Heidi A.; Romero, Juan A.; Custer, Joyce Olsen; Hymel, Ross W.; Krementz, Dan; Gobin, Derek; Harpring, Larry; Martinez-Rodriguez, Michael; Varble, Don; DiMaio, Jeff; Hudson, Stephen

    2016-01-01

    Containment/Surveillance (C/S) measures are critical to any verification regime in order to maintain Continuity of Knowledge (CoK). The Ceramic Seal project is research into the next generation technologies to advance C/S, in particular improving security and efficiency. The Ceramic Seal is a small form factor loop seal with improved tamper-indication including a frangible seal body, tamper planes, external coatings, and electronic monitoring of the seal body integrity. It improves efficiency through a self-securing wire and in-situ verification with a handheld reader. Sandia National Laboratories (SNL) and Savannah River National Laboratory (SRNL), under sponsorship from the U.S. National Nuclear Security Administration (NNSA) Office of Defense Nuclear Nonproliferation Research and Development (DNN R&D), have previously designed and have now fabricated and tested Ceramic Seals. Tests have occurred at both SNL and SRNL, with different types of tests occurring at each facility. This interim report will describe the Ceramic Seal prototype, the design and development of a handheld standalone reader and an interface to a data acquisition system, fabrication of the seals, and results of initial testing.

  17. Electronic seal

    International Nuclear Information System (INIS)

    Musyck, E.

    1981-01-01

    An electronic seal is presented for a volume such as container for fissile materials. The seal encloses a lock for barring the space as well as a device for the detection and the recording of the intervention of the lock. (AF)

  18. Ferrules seals

    Science.gov (United States)

    Smith, J.L.

    1984-07-10

    A device is provided for sealing an inner tube and an outer tube without excessively deforming the tubes. The device includes two ferrules which cooperate to form a vacuum-tight seal between the inner tube and outer tube and having mating surfaces such that overtightening is not possible. 3 figs.

  19. Sealing devices

    International Nuclear Information System (INIS)

    Coulson, R.A.

    1980-01-01

    A sealing device for minimising the leakage of toxic or radioactive contaminated environments through a biological shield along an opening through which a flexible component moves that penetrates the shield. The sealing device comprises an outer tubular member which extends over a length not less than the maximum longitudinal movement of the component along the opening. An inner sealing block is located intermediate the length of the component by connectors and is positioned in the bore of the outer tubular member to slide in the bore and effect a seal over the entire longitudinal movement of the component. The cross-section of the device may be circular and the block may be of polytetrafluoroethylene or of nylon impregnated with molybdenum or may be metallic. A number of the sealing devices may be combined into an assembly for a plurality of adjacent longitudinally movable components, each adapted to sustain a tensile load, providing the various drives of a master-slave manipulator. (author)

  20. Encapsulation of ethylhexyl methoxycinnamate, a light-sensitive UV filter, in lipid nanoparticles.

    Science.gov (United States)

    Durand, L; Habran, N; Henschel, V; Amighi, K

    2010-01-01

    The aim of this study was to encapsulate ethylhexyl methoxycinnamate (EMC), a commonly used UVB filter, in a solid lipid matrix in order to obtain microparticles and then nanoparticles to reduce its photo-instability under UV light exposure. Glyceryl behenate, rice bran wax and ozokerite were investigated for encapsulating EMC. The suspensions of nanoparticles contained 70% encapsulated EMC (relative to the lipid mass). The absorbance level at 310 nm of suspensions containing nanoparticles was more than twice that of those containing microparticles. So, decreasing the size of particles improved the efficiency of light protection, regardless of the lipid material used. Moreover, free EMC presented a 30% loss of its efficiency after 2 h of irradiation, whereas the three NLC formulations showed a loss of absorbency between 10% and 21%. The in vitro cutaneous penetration test did not show a higher potential penetration for EMC contained in nanosuspensions compared to free EMC.

  1. arXiv Wilson lines and UV sensitivity in magnetic compactifications

    CERN Document Server

    Ghilencea, D.M.

    2017-06-07

    We investigate the ultraviolet (UV) behaviour of 6D N=1 supersymmetric effective (Abelian) gauge theories compactified on a two-torus (T$_{2}$) with magnetic flux. To this purpose we compute offshell the one-loop correction to the Wilson line state self-energy. The offshell calculation is actually necessary to capture the usual effective field theory expansion in powers of (∂/Λ). Particular care is paid to the regularization of the (divergent) momentum integrals, which is relevant for identifying the corresponding counterterm(s). We find a counterterm which is a new higher dimensional effective operator of dimension d=6, that is enhanced for a larger compactification area (where the effective theory applies) and is consistent with the symmetries of the theory. Its consequences are briefly discussed and comparison is made with orbifold compactifications without flux.

  2. UV-sensitivity of bromodeoxyuridine (BUdR)-substituted chromosomes in Chinese hamster cells

    International Nuclear Information System (INIS)

    Antoshchina, M.M.; Luchnik, N.V.

    1990-01-01

    Chinese hamster cells with chromosomes differently substituted for BUdR (TT-TT, TT-TB, TB-TB, TB-BB, where T is thymidine containing chromatid and B is BUdR substituted chromatid) were exposed to UV-light in phase G 2 and chromosome aberrations (mainly chromatid breaks) were analysed. Breaks frequency per chromosome was proportional to BUdR content. No breaks were found in TT-TT chromosomes. The frequency of breaks per TB chromatid was similar with TT-TB and TB-BB chromosomes. In TB-BB chromosomes, however, virtually no breaks occured in TB chromatids whereas in BB chromatids, their frequency was much higher than was expected

  3. Study of the UV-sensitivity of the morphological Salmonella typhimurium mutant

    Energy Technology Data Exchange (ETDEWEB)

    Sakanyan, V A; Dombrovskii, A M; Belokrysenko, S S; Levashev, V S [Vtoroj Moskovskij Gosudarstvennyj Meditsinskij Inst. (USSR)

    1975-05-01

    As regards sensitivity to ultraviolet radiation, the morphological mutant S. typhimurium LT2 WT ED 143 is similar to the ion-mutants E. coli K12. Data are presented on the sensitivity of the mutant and initial strains to ultraviolet radiation at various phases of growth, on the capacity for restoring the bacteriophages P22 and Felix O after irradiation and on the influence of various treatments after ultraviolet irradiation (incubation in minimum media and at 42/sup 0/ C) on the irradiated strains. The results of densitometry of the membrane proteins of the initial and mutant strains point to a connection between unusual morphology, the disruption of division and the enhanced sensitivity to ultraviolet radiation on one hand and the state of the membrane components of the bacterial cell on the other.

  4. Secondary seal effects in hydrostatic non-contact seals for reactor coolant pump shaft

    International Nuclear Information System (INIS)

    Fujita, T.; Koga, T.; Tanoue, H.; Hirabayashi, H.

    1987-01-01

    The paper presents a seal flow analysis in a hydrostatic non-contact seal for a PWR coolant pump shaft. A description is given of the non-contact seal for the reactor coolant pump. Results are presented for a distortion analysis of the seal ring, along with the seal flow characteristics and the contact pressure profiles of the secondary seals. The results of the work confirm previously reported findings that the seal ring distortion is sensitive to the o-ring location (which was placed between the ceramic seal face and the seal ring retainer). The paper concludes that the seal flow characteristics and the tracking performance depend upon the dynamic properties of the secondary seal. (U.K.)

  5. Mutagenesis at the ad-3A and ad-3B loci in haploid UV-sensitive strains of Neurospora crassa. Pt. 6

    International Nuclear Information System (INIS)

    De Serres, F.J.; Inoue, H.; Schuepbach, M.E.

    1983-01-01

    Genetic characterization of ad-3B mutants induced in wild-type and UV-sensitive strains has revealed qualitative differences between the spectra of genetic alterations at the molecular level. Ad-3B mutants induced in the two nucleotide excision-repair-deficient strains upr-1 and uvs-2 had significantly lower frequencies of nonpolarized complementation patterns and higher frequencies of noncomplementing mutants than ad-3B mutants induced in the wild-type strain in samples induced by either UV, #betta#-rays, 4NQO or MNNG. In these same samples ad-3B mutants induced in uvs-4, uvs-5 or uvs-6 did not differ significantly from those induced in the wild-type strain. After ICR-170 treatment, ad-3B mutants induced in the UV-sensitive strains did not differ significantly from those induced in wild-type. The comparisons in the present and previous studies demonstrate that the process of mutation-induction in the ad-3 region is under the control of other loci that not only alter mutant recovery quantitatively but also qualitatively. These data have important implications for comparative chemical mutagenesis, since the spectrum of genetic alterations produced by a given agent can be modified markedly as a result of defects in DNA repair. (orig./AJ)

  6. Seals, Concrete Anchors, and Connections

    Science.gov (United States)

    1989-02-01

    caulking compounds, nonhardening extruded tapes, nonhardening mastics, strippable spray coatings, pressure sensitive tapes, gaskets, adhesives, fabrics...films, etc. Although all of these materials may provide a seal, care must be taken when selecting a sealing material as to its chemical and...gaskets have performed satisfactorily. Another factor to be considered in the selection of gasketing material is its compatibility with both the

  7. Enhancement of Rayleigh scatter in optical fiber by simple UV treatment: an order of magnitude increase in distributed sensing sensitivity

    Science.gov (United States)

    Loranger, Sébastien; Parent, François; Lambin-Iezzi, Victor; Kashyap, Raman

    2016-02-01

    Rayleigh scatter in optical fiber communication systems has long been considered a nuisance as a loss mechanism, although applications have used such scatter to probe the fiber for faults and propagation loss using time domain reflectometry (OTDR). It is however only with the development of Frequency domain reflectometry (OFDR) and coherent-phase OTDR that Rayleigh scatter has been probed to its deepest and can now be used to measure strain and temperature along a fiber, leading to the first distributed sensing applications. However, Rayleigh scatter remains very weak giving rise to very small signals which limits the technique for sensing. We show here a new technique to significantly enhance the Rayleigh scatter signal by at least two orders of magnitude, in a standard optical fiber with simple UV exposure of the core. A study of various exposures with different types of fibers has been conducted and a phenomenological description developed. We demonstrate that such an increase in signal can enhance the temperature and strain sensitivity by an order of magnitude for distributed sensing with an OFDR technique. Such improved performance can lead to temperature/strain RMS noise levels of 6 mK and 50 nɛ for 1 cm spatial resolution in UV exposed SMF-28, compared to the typical noise level of 100 mK for the same spatial resolution in the similar unexposed fiber.

  8. Cockayne's syndrome: correlation of clinical features with cellular sensitivity of RNA synthesis to UV irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Lehmann, A.R.; Thompson, A.F.; Harcourt, S.A. (Medical Research Council, Brighton (United Kingdom). Cell Mutation Unit); Stefanini, Miria (Consiglio Nazionale delle Ricerche, Pavia (Italy). Ist. di Genetica Biochimica ed Evoluzionistica); Norris, P.G. (Addenbrooke' s Hospital, Cambridge (United Kingdom))

    1993-08-01

    Cockayne's syndrome (CS) is a rare autosomal recessive disorder with dwarfism, mental retardation, and otherwise clinically heterogeneous features. In cultured CS fibroblasts, the failure of RNA synthesis to recover to normal rates after UV-C irradiation provides a useful and relatively simple diagnostic test. We have measured post-UV-C RNA synthesis in 52 patients for whom a clinical diagnosis of CS was considered a possibility. Twenty-nine patients showed the defect characteristic of CS cells, and 23 had a normal response. We have attempted to correlate the cellular diagnosis with the different clinical features of the disorder. Clinical details of the patients were obtained from referring clinicians in the form of a questionnaire. Our results show that, apart from the cardinal features of dwarfism and mental retardation, sun sensitivity correlated best with a positive cellular diagnosis. Pigmentary retinopathy, gait defects, and dental caries were also good positive indicators, although several patients with a positive cellular diagnosis did not have these features. (Author).

  9. Fabrication of a microfluidic chip by UV bonding at room temperature for integration of temperature-sensitive layers

    Science.gov (United States)

    Schlautmann, S.; Besselink, G. A. J.; Radhakrishna Prabhu, G.; Schasfoort, R. B. M.

    2003-07-01

    A method for the bonding of a microfluidic device at room temperature is presented. The wafer with the fluidic structures was bonded to a sensor wafer with gold pads by means of adhesive bonding, utilizing an UV-curable glue layer. To avoid filling the fluidic channels with the glue, a stamping process was developed which allows the selective application of a thin glue layer. In this way a microfluidic glass chip was fabricated that could be used for performing surface plasmon resonance measurements without signs of leakage. The advantage of this method is the possibility of integration of organic layers as well as other temperature-sensitive layers into a microfluidic glass device.

  10. Bactericidal activity under UV and visible light of cotton fabrics coated with anthraquinone-sensitized TiO2

    KAUST Repository

    Rahal, Raed

    2013-06-01

    This study describes a method derived from ISO/TC 206/SC specifications to assess the bactericidal activity against a bacterial strain, Pseudomonas fluorescens, of various photocatalytic fabrics, under UVA and filtered visible light. The experimental method allowed the accurate quantification of bacteria survival on photoactive surfaces and films under UVA and UV-free visible irradiation. Cotton fabrics coated with TiO2, anthraquinone or anthraquinone-sensitized TiO2 display a significant bactericidal efficiency. TiO2-coated fabrics are very efficient against P. fluorescens after 4 h UVA irradiation (bacteria survival below the detection limit). Under UVA-free visible light, anthraquinone-sensitized TiO2 coated fabrics induced a significant bactericidal activity after 2 h irradiation, while anthraquinone alone-coated fabrics were not as efficient and TiO2 coated fabrics were almost inefficient. These results show that although exhibiting a weak n-π* band in the 350-420 nm range, anthraquinone is a good candidate as an efficient visible light photosensitizer. A synergy effect between anthraquinone and TiO2 was demonstrated. A possible reaction mechanism, involving a synergy effect for singlet oxygen formation with anthraquinone-sensitized TiO2 is proposed to account for these results. © 2012 Elsevier B.V. All rights reserved.

  11. A spirooxazine derivative as a highly sensitive cyanide sensor by means of UV-visible difference spectroscopy.

    Science.gov (United States)

    Zhu, Shaoyin; Li, Minjie; Sheng, Lan; Chen, Peng; Zhang, Yumo; Zhang, Sean Xiao-An

    2012-12-07

    A spirooxazine derivative 2-nitro-5a-(2-(4-dimethylaminophenyl)-ethylene)-6,6-dimethyl-5a,6-dihydro-12H-indolo[2,1-b][1,3]benzooxazine (P1) was explored as a sensitive cyanide probe. Different from conventional spiropyrans, P1 avoided locating the 3H-indolium cation and the 4-nitrophenolate anion in the same conjugated structure, which enhanced the positive charge of 3H-indolium cation so that the sensitivity and reaction speed were improved highly. UV-visible difference spectroscopy using P1 detection solution as a timely reference improved the measurement accuracy, prevented the error caused by the inherent absorption change of P1 solution with time. This enabled the "positive-negative alternative absorption peaks" in difference spectrum to be used as a finger-print to distinguish whether the spectral change was caused by cyanide. Benefiting from the special design of the molecular structure and the strategy of difference spectroscopy, P1 showed high selectivity and sensitivity for CN(-). A detection limit of 0.4 μM and a rate constant of 1.1 s(-1) were achieved.

  12. Sensitization by UV light of α-Al2O3:C polycrystalline detectors

    International Nuclear Information System (INIS)

    Meira B, L. C.; Rubio F, H.; Neres de A, E.; Santos, A.

    2014-08-01

    This paper describes an increase in sensitivity to gamma and beta radiation on α-Al 2 O 3 :C polycrystalline detector, which has been produced by a sol-gel process, following previous exposure to ultraviolet light. The increased sensitivity of the detector as a function of the exposure time and ultraviolet wavelength was studied. Since the main luminescent centers have emission peaks at different wavelengths, selective measurements of thermoluminescent emission intensity were done, in order to investigate the possible conversion of centers as a result of the exposition to ultraviolet light. Experimental results indicate that the nature and parameters of the luminescent centers in α-Al 2 O 3 :C sol-gel material can be very different of those in α-Al 2 O 3 :C single crystal. (author)

  13. Spectrometer sensitivity calibration in the extreme uv by means of branching ratios of magnetic dipole lines

    International Nuclear Information System (INIS)

    Denne, B.; Hinnov, E.

    1984-04-01

    Relative intensity measurements of various line pairs resulting from magnetic dipole transitions within the configurations s 2 p 2 and s 2 p 4 , in conjunction with calculated transition probabilities, have been used to determine the wavelength dependence of the sensitivity of a grazing incidence spectrometer, in the range 400 to 1000 A. Emissions from Cr XIX, Fe XXI, Ni XXI and XXIII, Cu XXIV, and Zr XXVII ions in PLT tokamak discharges were used for this purpose. Absolute sensitivity of the spectrometer at selected wavelengths had been determined by the traditional hydrogen, helium, carbon, and oxygen electric-dipole line pairs from the same discharges. Similar attempts to use transitions in the s 2 p 3 configurations in Cr XVIII, Zr XXVI, and Mo XXVIII ions resulted in significant discrepancies that are ascribed to uncertainties in the corresponding calculated transition probabilities

  14. [Temperature sensitivity of wheat plant respiration and soil respiration influenced by increased UV-B radiation from elongation to flowering periods].

    Science.gov (United States)

    Chen, Shu-Tao; Hu, Zheng-Hua; Li, Han-Mao; Ji, Yu-Hong; Yang, Yan-Ping

    2009-05-15

    Field experiment was carried out in the spring of 2008 in order to investigate the effects of increased UV-B radiation on the temperature sensitivity of wheat plant respiration and soil respiration from elongation to flowering periods. Static chamber-gas chromatography method was used to measure ecosystem respiration and soil respiration under 20% UV-B radiation increase and control. Environmental factors such as temperature and moisture were also measured. Results indicated that supplemental UV-B radiation inhibited the ecosystem respiration and soil respiration from wheat elongation to flowering periods, and the inhibition effect was more obvious for soil respiration than for ecosystem respiration. Ecosystem respiration rates, on daily average, were 9%, 9%, 3%, 16% and 30% higher for control than for UV-B treatment forthe five measurement days, while soil respiration rates were 99%, 93%, 106%, 38% and 10% higher for control than for UV-B treatment. The Q10s (temperature sensitivity coefficients) for plant respiration under control and UV-B treatments were 1.79 and 1.59, respectively, while the Q10s for soil respiration were 1.38 and 1.76, respectively. The Q10s for ecosystem respiration were 1.65 and 1.63 under CK and UV-B treatments, respectively. Supplemental UV-B radiation caused a lower Q10 for plant respiration and a higher Q10 for soil respiration, although no significant effect of supplemental UV-B radiation on the Q10 for ecosystem respiration was found.

  15. High-Sensitivity Semiconductor Photocathodes for Space-Born UV Photon-Counting and Imaging, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — Many UV photon-counting and imaging applications, including space-borne astronomy, missile tracking and guidance, UV spectroscopy for chemical/biological...

  16. A UV-prepared linear polymer electrolyte membrane for dye-sensitized solar cells

    Energy Technology Data Exchange (ETDEWEB)

    Imperiyka, M., E-mail: imperiyka@gmail.com [Faculty of Arts and Sciences, Kufra Campus, University of Benghazi, Al Kufrah (Libya); Ahmad, A.; Hanifah, S.A. [School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor (Malaysia); Polymer Research Center, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor (Malaysia); Bella, F. [Center for Space Human Robotics @Polito, Istituto Italiano di Tecnologia, Corso Trento 21, 10129 Torino (Italy); Department of Applied Science and Technology – DISAT, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino (Italy)

    2014-10-01

    The effects of LiClO{sub 4} and LiFS{sub 3}SO{sub 3} on poly(glycidyl methacrylate)-based solid polymer electrolyte and its photoelectrochemical performance in a dye sensitized solar cell consisting of FTO/TiO{sub 2}–dye/P(GMA)–LiClO{sub 4}–EC/Pt were investigated. The electrochemical stability of films was studied by cyclic voltammetry (CV). The highest ionic conductivities obtained were 4.2×10{sup −5} and 3.7×10{sup −6} S cm{sup −1} for the film containing 30 wt% LiClO{sub 4} and 25 wt% LiCF{sub 3}SO{sub 3}, respectively. The polymer electrolytes showed electrochemical stability windows up to 3 V and 2.8 V for LiClO{sub 4} and LiCF{sub 3}SO{sub 3}, respectively. The assembled dye-sensitized solar cell showed a sunlight conversion efficiency of 0.679% (J{sub sc}=3 mA cm{sup −2}, V{sub oc}=0.48 V and FF=0.47), under light intensity of 100 mW cm{sup −2}.

  17. Basis for slow growth on non-fermentable substrates by a saccharomyces cerevisiae mutant UV-sensitive for rho- production

    International Nuclear Information System (INIS)

    Crosby, B.; Colson, A.M.; Briquet, M.; Goffeau, A.; Moustacchi, E.

    1978-01-01

    The mutant uvsp 72 of Saccharomyces cerevisiae UV-sensitive for rho - production displays slower growth on media containing non-fermentable carbon sources such as glycerol or lactate. The slower growth on glycerol is not due to any deficiency in glycerol catabolism or mitochondrial oxidative phosphorylation. No modifications of the sensitivity to ethidium bromide of the mitochondrial ATPase activity could be detected. A mathematical model is presented which accounts for slower growth of uvsp 72 on the sole basis of the continuous and elevated rho - production in the mutant strain. This model, which estimates the rate of mutation from the rate of growth and vice versa, has been verified experimentally in the case of uvsp 72. The model has been generalised, so that it can be used for any microbial population subject to constant and high rates of any type of mutation providing that the mutant is stable, and either unable to grow or able to grow at this own rate different from that of the parental strain. (orig.) [de

  18. Caffeine sensitive repair and mutation induction in UV- or γ-ray-irradiated Dictyostelium discoideum

    International Nuclear Information System (INIS)

    Kanishi, Nobuji; Kinjo, Yasuhito; Watanabe, Makoto.

    1990-01-01

    It seems that certain kinds of chemical substances increase the distortion in molecules, change the high order microstructures of nuclei and chromosomes, and exert large variation to the function of repairing the damage of genes due to radiation and others, by coupling with DNA, protein or enzyme system. It has been well known that caffeine is one of such compounds, and by coupling with DNA, it increases the damage due to ultraviolet ray and gives the action of obstructing repair in addition to the action of inducing the abnormality of chromosomes and mutation. Dictyostelium discoideum has the simplest nuclear structure, and shows extremely high resistance to radiation by its high restoration ability. The authors have advanced the research by paying attention to its characteristics, and comparing the Dictyostelium discoideum as one model system with the lymphocyte system of higher animals. This time, the authors analyzed the characteristics of two kinds of sensitivity repair process of caffeine, and investigated into their relation with the occurrence of mutation. The experimental method and the results are reported. (K.I.)

  19. UV sensitivity of planktonic net community production in ocean surface waters

    Science.gov (United States)

    Regaudie-de-Gioux, Aurore; Agustí, Susana; Duarte, Carlos M.

    2014-05-01

    The net plankton community metabolism of oceanic surface waters is particularly important as it more directly affects the partial pressure of CO2 in surface waters and thus the air-sea fluxes of CO2. Plankton communities in surface waters are exposed to high irradiance that includes significant ultraviolet blue (UVB, 280-315 nm) radiation. UVB radiation affects both photosynthetic and respiration rates, increase plankton mortality rates, and other metabolic and chemical processes. Here we test the sensitivity of net community production (NCP) to UVB of planktonic communities in surface waters across contrasting regions of the ocean. We observed here that UVB radiation affects net plankton community production at the ocean surface, imposing a shift in NCP by, on average, 50% relative to the values measured when excluding partly UVB. Our results show that under full solar radiation, the metabolic balance shows the prevalence of net heterotrophic community production. The demonstration of an important effect of UVB radiation on NCP in surface waters presented here is of particular relevance in relation to the increased UVB radiation derived from the erosion of the stratospheric ozone layer. Our results encourage design future research to further our understanding of UVB effects on the metabolic balance of plankton communities.

  20. Caffeine sensitive repair and mutation induction in UV- or. gamma. -ray-irradiated Dictyostelium discoideum

    Energy Technology Data Exchange (ETDEWEB)

    Kanishi, Nobuji (Tokyo Metropolitan Research Lab. of Public Health (Japan)); Kinjo, Yasuhito; Watanabe, Makoto

    1990-01-01

    It seems that certain kinds of chemical substances increase the distortion in molecules, change the high order microstructures of nuclei and chromosomes, and exert large variation to the function of repairing the damage of genes due to radiation and others, by coupling with DNA, protein or enzyme system. It has been well known that caffeine is one of such compounds, and by coupling with DNA, it increases the damage due to ultraviolet ray and gives the action of obstructing repair in addition to the action of inducing the abnormality of chromosomes and mutation. Dictyostelium discoideum has the simplest nuclear structure, and shows extremely high resistance to radiation by its high restoration ability. The authors have advanced the research by paying attention to its characteristics, and comparing the Dictyostelium discoideum as one model system with the lymphocyte system of higher animals. This time, the authors analyzed the characteristics of two kinds of sensitivity repair process of caffeine, and investigated into their relation with the occurrence of mutation. The experimental method and the results are reported. (K.I.).

  1. The effect of caffeine on post-replication repair and survival in two L5178Y cell lines with different sensitivities to UV irradiation

    International Nuclear Information System (INIS)

    Walicka, M.; Beer, J.Z.; Koerner, I.; Malz, W.

    1978-01-01

    2 Strains of murine lymphoma L5178Y cells that varied from the point of view of sensitivity to UV irradiation (mean lethal doses: 3.6 and 8.5 J/m 2 for L5178Y-R and L5178Y-S cells, respectively) also differed with respect to sensitization by caffeine. L5178Y-S cells were sensitized to UV irradiation by 0.75 mM caffeine, whereas in the same conditions L5178Y-R cells were not sensitized. Sedimentation analysis of the newly synthesized DNA indicated UV-induced gap formation in L5178Y-S cells only. The subsequent gap filling was inhibited by caffeine. Exposure to UV irradiation induced no gaps in L5178Y-R cells. However, when caffeine was added immediately after irradiation, DNA with reduced molecular weight was found in irradiated cells of both strains after a 2-h chase. On the other hand, caffeine inhibited elongation of undamaged DNA strands in neither of the 2 cell strains. (Auth.)

  2. Effects of ultraviolet irradiation on the cell cycle in normal and UV-sensitive cell lines with reference to the nature of the defect in xeroderma pigmentosum variant

    International Nuclear Information System (INIS)

    Imray, P.; Mangan, T.; Saul, A.; Kidson, C.

    1983-01-01

    Analysis of the distribution of cells through the phases of the cell cycle by DNA flow cytofluorimetry has been utilized to investigate the effects of ultraviolet (UV) irradiation on cell-cycle progression in normal and UV-sensitive lymphoblastoid cell lines. In time-course studies only slight perturbation of DNA distribution was seen in normal cells, or UV-sensitive familial melanoma (FM) lines in the 48 h following irradiation. Xeroderma pigmentosum (XPA) excision-deficient cells showed a large increase in the proportion of cells in S phase 16-40 h post-irradiation. XP variant (XPV) cells were blocked in G 1 and S phases with the complete absence of cells with G 2 DNA content 16-28 h after irradiation. By 48 h post-irradiation the DNA distribution of XPA and XPV cells had returned to that of an unirradiated control. When colcemid was added to the cultures immediately after irradiation to prevent mitotic cells dividing and re-entering the cell cycle, progression through the first cycle after irradiation was followed. UV irradiation did not affect the rate of movement of cells out of G 1 into S phase in normal, FM or XPA cells. The proportion of cells in S phase was increased in UV-irradiated cultures in these cell types and the number of cells entering the G 2 +M compartment was reduced. (orig./AJ)

  3. Ultraporous Electron-Depleted ZnO Nanoparticle Networks for Highly Sensitive Portable Visible-Blind UV Photodetectors.

    Science.gov (United States)

    Nasiri, Noushin; Bo, Renheng; Wang, Fan; Fu, Lan; Tricoli, Antonio

    2015-08-05

    A hierarchical nano- and microstructured morphology for visible-blind UV photo-detectors is developed, which provides record-high milliampere photocurrents, nanoampere dark currents, and excellent selectivity to ultralow UV light intensities. This is a significant step toward the integration of high-performance UV photodetectors in wearable devices. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. Sensitive determination of trace mercury by UV-visible diffuse reflectance spectroscopy after complexation and membrane filtration-enrichment.

    Science.gov (United States)

    Yin, Changhai; Iqbal, Jibran; Hu, Huilian; Liu, Bingxiang; Zhang, Lei; Zhu, Bilin; Du, Yiping

    2012-09-30

    A simple, sensitive and selective solid phase reflectometry method is proposed for the determination of trace mercury in aqueous samples. The complexation reagent dithizone was firstly injected into the properly buffered solution with vigorous stirring, which started a simultaneous formation of nanoparticles suspension of dithizone and its complexation reaction with the mercury(II) ions to make Hg-dithizone nanoparticles. After a definite time, the mixture was filtered with membrane, and then quantified directly on the surface of the membrane by using integrating sphere accessory of the UV-visible spectrophotometer. The quantitative analysis was carried out at a wavelength of 485 nm since it yielded the largest difference in diffuse reflectance spectra before and after reaction with mercury(II).A good linear correlation in the range of 0.2-4.0 μg/L with a squared correlation coefficient (R(2)) of 0.9944 and a detection limit of 0.12 μg/L were obtained. The accuracy of the method was evaluated by the analysis of spiked mercury(II) concentrations determined using this method along with those determined by the atomic fluorescence mercury vapourmeter and the results obtained were in good agreement. The proposed method was applied to the determination of mercury in tap water and river water samples with the recovery in an acceptable range (95.7-105.3%). Copyright © 2012 Elsevier B.V. All rights reserved.

  5. Calibration of the OPAL jet chamber with UV laser beams. Measurement of the beam position with position-sensitive silicon diodes (PSD)

    International Nuclear Information System (INIS)

    Koch, J.

    1990-03-01

    The OPAL jet chamber is calibrated with tracks produced by UV laser beams. Lateral effect diodes are used for monitoring the laser beam location in the detector. These position sensitive detectors locate the point of impact in two dimensions by the charge division method. Measurements on several diodes were carried out in order to calibrate these devices and to investigate to observed pin-cushion distortion. Using the telegraphers equation suitable expressions were obtained for describing the observed behaviour. It was shown that the magnetic field of OPAL as well as the UV laser wavelength and puls duration had no influence on the position information. (orig.)

  6. The sensitivity of sunflower (Helianthus annuus L.) plants to UV-B radiation is altered by nitrogen status

    OpenAIRE

    Cechin, Inês; Gonzalez, Gisely Cristina; Corniani, Natália; Fumis, Terezinha de Fátima

    2018-01-01

    ABSTRACT: Interaction effects between nitrogen and UV-B radiation were studied in sunflower (Helianthus annuus L. variety IAC-Iarama) plants grown in a greenhouse under natural photoperiod conditions. Plants were irradiated with 0.8W m-2 (control) or 8.0W m-2 (+UV-B) of UV-B radiation for 7h per day. The plants were grown in pots containing vermiculite and watered with 70% of full strength nitrogen-free Long Ashton solution, containing either low (42.3ppm) or high (282ppm) nitrogen as ammoniu...

  7. Positive selection of a duplicated UV-sensitive visual pigment coincides with wing pigment evolution in Heliconius butterflies

    OpenAIRE

    Briscoe, Adriana D.; Bybee, Seth M.; Bernard, Gary D.; Yuan, Furong; Sison-Mangus, Marilou P.; Reed, Robert D.; Warren, Andrew D.; Llorente-Bousquets, Jorge; Chiao, Chuan-Chin

    2010-01-01

    The butterfly Heliconius erato can see from the UV to the red part of the light spectrum with color vision proven from 440 to 640 nm. Its eye is known to contain three visual pigments, rhodopsins, produced by an 11-cis-3-hydroxyretinal chromophore together with long wavelength (LWRh), blue (BRh) and UV (UVRh1) opsins. We now find that H. erato has a second UV opsin mRNA (UVRh2)—a previously undescribed duplication of this gene among Lepidoptera. To investigate its evolutionary origin, we scre...

  8. Estratégias para aumento de sensibilidade em espectrofotometria UV-VIS Strategies to increase sensitivity in UV-VIS spectrophotometry

    Directory of Open Access Journals (Sweden)

    Fábio R. P. Rocha

    2004-10-01

    Full Text Available Spectrophotometry is one of the most widespread analytical techniques due to its simplicity, reliability, and low-cost instrumentation for both direct measurements and coupled to other techniques or processes such as chromatography, electrophoresis and flow analysis. However, the application is often limited by sensitivity. This article describes some advances that greatly improve the performance of spectrophotometric measurements, especially in order to increase sensitivity, including the employment of liquid-core waveguides and solid-phase spectrophotometry.

  9. UV-Sensitivity of Shiga Toxin-Converting Bacteriophage Virions Φ24B, 933W, P22, P27 and P32

    Directory of Open Access Journals (Sweden)

    Sylwia Bloch

    2015-09-01

    Full Text Available Shiga toxin-converting bacteriophages (Stx phages are present as prophages in Shiga toxin-producing Escherichia coli (STEC strains. Theses phages can be transmitted to previously non-pathogenic E. coli cells making them potential producers of Shiga toxins, as they bear genes for these toxins in their genomes. Therefore, sensitivity of Stx phage virions to various conditions is important in both natural processes of spreading of these viruses and potential prophylactic control of appearance of novel pathogenic E. coli strains. In this report we provide evidence that virions of Stx phages are significantly more sensitive to UV irradiation than bacteriophage λ. Following UV irradiation of Stx virions at the dose of 50 J/m2, their infectivity dropped by 1–3 log10, depending on the kind of phage. Under these conditions, a considerable release of phage DNA from virions was observed, and electron microscopy analyses indicated a large proportion of partially damaged virions. Infection of E. coli cells with UV-irradiated Stx phages resulted in significantly decreased levels of expression of N and cro genes, crucial for lytic development. We conclude that inactivation of Stx virions caused by relatively low dose of UV light is due to damage of capsids that prevents effective infection of the host cells.

  10. Vertical profile of tropospheric ozone derived from synergetic retrieval using three different wavelength ranges, UV, IR, and microwave: sensitivity study for satellite observation

    Science.gov (United States)

    Sato, Tomohiro O.; Sato, Takao M.; Sagawa, Hideo; Noguchi, Katsuyuki; Saitoh, Naoko; Irie, Hitoshi; Kita, Kazuyuki; Mahani, Mona E.; Zettsu, Koji; Imasu, Ryoichi; Hayashida, Sachiko; Kasai, Yasuko

    2018-03-01

    We performed a feasibility study of constraining the vertical profile of the tropospheric ozone by using a synergetic retrieval method on multiple spectra, i.e., ultraviolet (UV), thermal infrared (TIR), and microwave (MW) ranges, measured from space. This work provides, for the first time, a quantitative evaluation of the retrieval sensitivity of the tropospheric ozone by adding the MW measurement to the UV and TIR measurements. Two observation points in East Asia (one in an urban area and one in an ocean area) and two observation times (one during summer and one during winter) were assumed. Geometry of line of sight was nadir down-looking for the UV and TIR measurements, and limb sounding for the MW measurement. The retrieval sensitivities of the ozone profiles in the upper troposphere (UT), middle troposphere (MT), and lowermost troposphere (LMT) were estimated using the degree of freedom for signal (DFS), the pressure of maximum sensitivity, reduction rate of error from the a priori error, and the averaging kernel matrix, derived based on the optimal estimation method. The measurement noise levels were assumed to be the same as those for currently available instruments. The weighting functions for the UV, TIR, and MW ranges were calculated using the SCIATRAN radiative transfer model, the Line-By-Line Radiative Transfer Model (LBLRTM), and the Advanced Model for Atmospheric Terahertz Radiation Analysis and Simulation (AMATERASU), respectively. The DFS value was increased by approximately 96, 23, and 30 % by adding the MW measurements to the combination of UV and TIR measurements in the UT, MT, and LMT regions, respectively. The MW measurement increased the DFS value of the LMT ozone; nevertheless, the MW measurement alone has no sensitivity to the LMT ozone. The pressure of maximum sensitivity value for the LMT ozone was also increased by adding the MW measurement. These findings indicate that better information on LMT ozone can be obtained by adding constraints

  11. Vertical profile of tropospheric ozone derived from synergetic retrieval using three different wavelength ranges, UV, IR, and microwave: sensitivity study for satellite observation

    Directory of Open Access Journals (Sweden)

    T. O. Sato

    2018-03-01

    Full Text Available We performed a feasibility study of constraining the vertical profile of the tropospheric ozone by using a synergetic retrieval method on multiple spectra, i.e., ultraviolet (UV, thermal infrared (TIR, and microwave (MW ranges, measured from space. This work provides, for the first time, a quantitative evaluation of the retrieval sensitivity of the tropospheric ozone by adding the MW measurement to the UV and TIR measurements. Two observation points in East Asia (one in an urban area and one in an ocean area and two observation times (one during summer and one during winter were assumed. Geometry of line of sight was nadir down-looking for the UV and TIR measurements, and limb sounding for the MW measurement. The retrieval sensitivities of the ozone profiles in the upper troposphere (UT, middle troposphere (MT, and lowermost troposphere (LMT were estimated using the degree of freedom for signal (DFS, the pressure of maximum sensitivity, reduction rate of error from the a priori error, and the averaging kernel matrix, derived based on the optimal estimation method. The measurement noise levels were assumed to be the same as those for currently available instruments. The weighting functions for the UV, TIR, and MW ranges were calculated using the SCIATRAN radiative transfer model, the Line-By-Line Radiative Transfer Model (LBLRTM, and the Advanced Model for Atmospheric Terahertz Radiation Analysis and Simulation (AMATERASU, respectively. The DFS value was increased by approximately 96, 23, and 30 % by adding the MW measurements to the combination of UV and TIR measurements in the UT, MT, and LMT regions, respectively. The MW measurement increased the DFS value of the LMT ozone; nevertheless, the MW measurement alone has no sensitivity to the LMT ozone. The pressure of maximum sensitivity value for the LMT ozone was also increased by adding the MW measurement. These findings indicate that better information on LMT ozone can be obtained by adding

  12. Sacrificial hydrogen generation from aqueous triethanolamine with Eosin Y-sensitized Pt/TiO2 photocatalyst in UV, visible and solar light irradiation.

    Science.gov (United States)

    Chowdhury, Pankaj; Gomaa, Hassan; Ray, Ajay K

    2015-02-01

    In this paper, we have studied Eosin Y-sensitized sacrificial hydrogen generation with triethanolamine as electron donor in UV, visible, and solar light irradiation. Aeroxide TiO2 was loaded with platinum metal via solar photo-deposition method to reduce the electron hole recombination process. Photocatalytic sacrificial hydrogen generation was influenced by several factors such as platinum loading (wt%) on TiO2, solution pH, Eosin Y to Pt/TiO2 mass ratio, triethanolamine concentration, and light (UV, visible and solar) intensities. Detailed reaction mechanisms in visible and solar light irradiation were established. Oxidation of triethanolamine and formaldehyde formation was correlated with hydrogen generation in both visible and solar lights. Hydrogen generation kinetics followed a Langmuir-type isotherm with reaction rate constant and adsorption constant of 6.77×10(-6) mol min(-1) and 14.45 M(-1), respectively. Sacrificial hydrogen generation and charge recombination processes were studied as a function of light intensities. Apparent quantum yields (QYs) were compared for UV, visible, and solar light at four different light intensities. Highest QYs were attained at lower light intensity because of trivial charge recombination. At 30 mW cm(-2) we achieved QYs of 10.82%, 12.23% and 11.33% in UV, visible and solar light respectively. Copyright © 2014 Elsevier Ltd. All rights reserved.

  13. Improved circumferential shaft seal

    Science.gov (United States)

    Ludwig, L. P.; Strom, T. N.

    1974-01-01

    Comparative tests of modified and unmodified carbon ring seals showed that addition of helical grooves to conventional segmented carbon ring seals reduced leakage significantly. Modified seal was insensitive to shaft runout and to flooding by lubricant.

  14. uvsI mutants defective in UV mutagenesis define a fourth epistatic group of uvs genes in Aspergillus.

    Science.gov (United States)

    Chae, S K; Kafer, E

    1993-01-01

    Three UV-sensitive mutations of A. nidulans, uvsI, uvsJ and uvsA, were tested for epistatic relationships with members of the previously established groups, here called the "UvsF", "UvsC", and "UvsB" groups. uvsI mutants are defective for spontaneous and induced reversion of certain point mutations and differ also for other properties from previously analyzed uvs types. They are very sensitive to the killing effects of UV-light and 4-NQO (4-nitro-quinoline-N-oxide) but not to MMS (methylmethane sulfonate). When double- and single-mutant uvs strains were compared for sensitivity to these three agents, synergistic or additive effects were found for uvsI with all members of the three groups. The uvsI gene may therefore represent a fourth epistatic group, possibly involved in mutagenic repair. On the other hand, uvsJ was clearly epistatic with members of the UvsF group and fitted well into this group also by phenotype. The uvsA gene was tentatively assigned to the UvsC group. uvsA showed epistatic interactions with uvsC in all tests, and like UvsC-group mutants is UV-sensitive mainly in dividing cells. However, the uvsA mutation does not cause the defects in recombination and UV mutagenesis typical for this group.

  15. Inactivation of normal and mutant Neurospora crassa conidia by visible light and near-UV: role of 1O2, carotenoid composition and sensitizer location

    International Nuclear Information System (INIS)

    Thomas, S.A.; Sargent, M.L.; Tuveson, R.W.

    1981-01-01

    Inactivation of Neurospora crassa conidia from wild-type and mutant strains by visible and near-ultraviolet light was investigated in the presence and absence of photosensitizing dyes. Inactivation by near-UV was virtually unchanged by the presence of deuterium oxide or azide suggesting that, contrary to the situation with visible light and photosensitizing dyes, 1 O 2 is not involved in any substantial way in the formation of lethal lesions. Carotenoid deficient strains were similar to wild-type strains in sensitivity to near-UV inactivation which is consistent with 1 O 2 not being involved. Photodynamic inactivation of conidia by visible light occurred in the presence of methylene blue (MB), toluidine blue O (TB), or acridine orange (AO). Carotenoid deficient strains were more sensitive to such inactivation only when MB and TB were used. This suggests that MB and TB mediated damage involves the cell membrane where carotenoids are available for quenching, whereas AO mediated damage occurs in the nucleus sequestered from the protective influence of carotenoids. A newly isolated, lemon-yellow mutant exhibited sensitivities to photodynamic inactivation similar to other pure-white mutants. The sensitivity of this pigmented mutant is apparently related to insufficient unsaturation of the two coloured carotenoids produced by the mutant. (author)

  16. Influence of the UV radiation on the screen-printed pH-sensitive layers based on graphene and ruthenium dioxide

    Science.gov (United States)

    Pepłowski, A.; Grudziński, D.; Raczyński, T.; Wróblewski, G.; Janczak, D.; Jakubowska, M.

    2017-08-01

    Electrodes for measuring pH of the solution were fabricated by the means of screen-printing technology. Potentiometric sensors' layers comprised of composite with polymer matrix and graphene nanoplatelets/ruthenium (IV) oxide nanopowder as functional phase. Transceivers were printed on the elastic PMMA foil. Regarding potential application of the sensors in the wearable devices, dynamic response of the electrodes to changing ultraviolet radiation levels was assessed, since RuO2 is reported to be UV-sensitive. Observed changes of the electrodes' potential were of sub-millivolt magnitude, being comparable to simultaneously observed signal drift. Given this stability under varying UV conditions and previously verified good flexibility, fabricated sensors meet the requirements for wearable applications.

  17. Comparison of UV-Curing, Hotmelt, and Pressure Sensitive Adhesive as Roll-to-Roll Encapsulation Methods for Polymer Solar Cells

    DEFF Research Database (Denmark)

    Hösel, Markus; Søndergaard, Roar R.; Jørgensen, Mikkel

    2013-01-01

    The most distinct advantage of the polymer solar cell is the possibility for roll-to-roll (R2R) fabrication compatibility based on printing and coating processes. The R2R encapsulation is the last crucial process step in the manufacturing workflow and is evaluated in this study. Polymer solar cell...... modules are directly printed on barrier foil and encapsulated with the same barrier foil either on the backside or on both sides of the device. The three lamination methods comprise of UV-curable epoxy resin, hotmelt, and pressure sensitive adhesive (PSA). It is shown that a single sided encapsulation...... of the PSA method is the fastest method (in this study) it generates a considerable amount of waste (paper liner) and two lamination steps are required to achieve a sufficient lifetime. We conclude that UV-curing presents the largest advantages of all methods with a good upscaling potential and low embodied...

  18. DNA degradation, UV sensitivity and SOS-mediated mutagenesis in strains of Escherichia coli deficient in single-strand DNA binding protein: Effects of mutations and treatments that alter levels of exonuclease V or RecA protein

    International Nuclear Information System (INIS)

    Lieberman, H.B.; Witkin, E.M.

    1983-01-01

    Certain strains suppress the temperature-sensitivity caused by ssb-1, which encodes a mutant ssDNA binding protein (SSB). At 42 0 C, such strains are extremely UV-sensitive, degrade their DNA extensively after UV irradiation, and are defficient in UV mutability and UV induction of recA protein synthesis. We transduced recC22, which eliminates Exonuclease V activity, and recAo281, which causes operator-constitutive synthesis of recA protein, into such an ssb-1 strain. Both double mutants degraded their DNA extensively at 42 0 C after UV irradiation, and both were even more UV-sensitive than the ssb-1 single mutant. We conclude that one or more nucleases other than Exonuclease V degrades DNA in the ssb recC strain, and that recA protein, even if synthesized copiously, can function efficiently in recombinational DNA repair and in control of post-UV DNA degradation only if normal SSB is also present. Pretreatment with nalidixic acid at 30 0 C restored normal UV mutability at 42 0 C, but did not increase UV resistance, in an ssb-1 strain. Another ssb allele, ssb-113, which blocks SOS induction at 30 0 C, increases spontaneous mutability more than tenfold. The ssb-113 allele was transduced into the SOS-constitutive recA730 strain SC30. This double mutant expressed the same elevated spontaneous and UV-induced mutability at 30 0 C as the ssb + recA730 strain, and was three times more UV-resistant than its ssb-113 recA + parent. We conclude that ssb-1 at 42 0 C and ssb-113 at 30 0 C block UV-induced activation of recA protease, but that neither allele interferes with subsequent steps in SOS-mediated mutagenesis. (orig.)

  19. Hydrodynamic perception in true seals (Phocidae) and eared seals (Otariidae).

    Science.gov (United States)

    Hanke, Wolf; Wieskotten, Sven; Marshall, Christopher; Dehnhardt, Guido

    2013-06-01

    Pinnipeds, that is true seals (Phocidae), eared seals (Otariidae), and walruses (Odobenidae), possess highly developed vibrissal systems for mechanoreception. They can use their vibrissae to detect and discriminate objects by direct touch. At least in Phocidae and Otariidae, the vibrissae can also be used to detect and analyse water movements. Here, we review what is known about this ability, known as hydrodynamic perception, in pinnipeds. Hydrodynamic perception in pinnipeds developed convergently to the hydrodynamic perception with the lateral line system in fish and the sensory hairs in crustaceans. So far two species of pinnipeds, the harbour seal (Phoca vitulina) representing the Phocidae and the California sea lion (Zalophus californianus) representing the Otariidae, have been studied for their ability to detect local water movements (dipole stimuli) and to follow hydrodynamic trails, that is the water movements left behind by objects that have passed by at an earlier point in time. Both species are highly sensitive to dipole stimuli and can follow hydrodynamic trails accurately. In the individuals tested, California sea lions were clearly more sensitive to dipole stimuli than harbour seals, and harbour seals showed a superior trail following ability as compared to California sea lions. Harbour seals have also been shown to derive additional information from hydrodynamic trails, such as motion direction, size and shape of the object that caused the trail (California sea lions have not yet been tested). The peculiar undulated shape of the harbour seals' vibrissae appears to play a crucial role in trail following, as it suppresses self-generated noise while the animal is swimming.

  20. Effects of age and liquid holding on the UV-radiation sensitivities of wild-type and mutant Caenorhabditis elegans dauer larvae

    Energy Technology Data Exchange (ETDEWEB)

    Hartman, P S

    1984-01-01

    The dauer larva is a facultative developmental stage in the life cycle of the nematode Caenorhabditis elegans. Dauer larvae, which can survive under starvation for over 60 days, resume normal development when feeding is resumed. Wild-type (N2) and 4 radiation-sensitive (rad) mutant dauer larvae were tested for their abilities to develop into adults after UV-irradiation. The rad-3 mutant was over 30 times as sensitive as N2; rad-1, rad-2 and rad-7 mutants were not hypersensitive. Irradiation also delayed development in survivors. Wild-type dauer larvae did not differ in radiation sensitivity from 0 through 50 days of age. There was no liquid holding recovery (LHR); that is, survival did not increase when wild-type dauer larvae were held in buffer after irradiation. (orig.). 28 refs.; 4 figs.

  1. Inhibition and recovery of semiconservative DNA synthesis in normal and solar UV sensitive ICR 2A frog cell lines following the induction of non-dimer DNA damage by sunlamp UV > 315 nm

    Energy Technology Data Exchange (ETDEWEB)

    Rosenstein, B.S. (Brown Univ., Providence, RI (USA). Dept. of Radiation Medicine)

    1989-08-01

    Cultures of solar UV-sensitive cell lines DRP 36 and DRP 153, and of the parental ICR 2A cell line, were exposed to 150 kJ/m{sup 2} of sunlamp UV>315nm plus photoreactivating light, resulting in the induction primarily of non-dimer DNA damage. Following either 0, 3, 6, 12 or 24 h incubation, cultures were pulse-labelled with ({sup 3}H) thymidine, and the synthesis of different size classes of replicon intermediates measured using alkaline step elution assay. For all three cell lines tested, an immediate depression of low molecular weight DNA synthesis was observed, followed by inhibition of all size classes of replicon intermediates. Within 12 h following irradiation, recovery of DNA synthesis was observed, generally most apparent for low molecular weight DNA. The ICR 2A cells exhibited a nearly full recovery in all size classes of DNA synthesized by 24 h. A much smaller recovery of continued inhibition was primarily in the synthesis of full replicon size DNA, and most pronounced for DRP 36 cells. (author).

  2. Rotary plug seal

    International Nuclear Information System (INIS)

    Ito, Koji; Abiko, Yoshihiro.

    1981-01-01

    Purpose: To enable fuel exchange even upon failure of regular seals and also to enable safety seal exchange by the detection of the reduction in the contact pressure of a rotary plug seal. Constitution: If one of a pair of regular tube seals for the rotary plug is failed during ordinary operation of a FBR type reactor, the reduction in the contact pressure of the seal to the plug gibbousness is detected by a pressure gauge and a solenoid valve is thereby closed. Thus, a back-up-tube seal provided above or below the tube seal is press-contacted by way of argon gas to the gibbousness to enter into operation state and lubricants are supplied from an oil tank. In such a structure, the back-up-tube seal is operated before the failure of the tube seal to enable to continue the fuel exchange work, as well as safety exchange for the tube seal. (Moriyama, K.)

  3. Seal design alternatives study

    International Nuclear Information System (INIS)

    Van Sambeek, L.L.; Luo, D.D.; Lin, M.S.; Ostrowski, W.; Oyenuga, D.

    1993-06-01

    This report presents the results from a study of various sealing alternatives for the WIPP sealing system. Overall, the sealing system has the purpose of reducing to the extent possible the potential for fluids (either gas or liquid) from entering or leaving the repository. The sealing system is divided into three subsystems: drift and panel seals within the repository horizon, shaft seals in each of the four shafts, and borehole seals. Alternatives to the baseline configuration for the WIPP seal system design included evaluating different geometries and schedules for seal component installations and the use of different materials for seal components. Order-of-magnitude costs for the various alternatives were prepared as part of the study. Firm recommendations are not presented, but the advantages and disadvantages of the alternatives are discussed. Technical information deficiencies are identified and studies are outlined which can provide required information

  4. Mechanical seal assembly

    Science.gov (United States)

    Kotlyar, Oleg M.

    2001-01-01

    An improved mechanical seal assembly is provided for sealing rotating shafts with respect to their shaft housings, wherein the rotating shafts are subject to substantial axial vibrations. The mechanical seal assembly generally includes a rotating sealing ring fixed to the shaft, a non-rotating sealing ring adjacent to and in close contact with the rotating sealing ring for forming an annular seal about the shaft, and a mechanical diode element that applies a biasing force to the non-rotating sealing ring by means of hemispherical joint. The alignment of the mechanical diode with respect to the sealing rings is maintained by a series of linear bearings positioned axially along a desired length of the mechanical diode. Alternative embodiments include mechanical or hydraulic amplification components for amplifying axial displacement of the non-rotating sealing ring and transferring it to the mechanical diode.

  5. Mechanical Seal Assembly

    Energy Technology Data Exchange (ETDEWEB)

    Kotlyar, Oleg M.

    1999-06-18

    An improved mechanical seal assembly is provided for sealing rotating shafts with respect to their shaft housings, wherein the rotating shafts are subject to substantial axial vibrations. The mechanical seal assembly generally includes a rotating sealing ring fixed to the shaft, a non-rotating sealing ring adjacent to and in close contact with the rotating sealing ring for forming an annular seal about the shaft, and a mechanical diode element that applies a biasing force to the non-rotating sealing ring by means of hemispherical joint. The alignment of the mechanical diode with respect to the sealing rings is maintained by a series of linear bearings positioned axially along a desired length of the mechanical diode. Alternative embodiments include mechanical or hydraulic amplification components for amplifying axial displacement of the non-rotating sealing ring and transferring it to the mechanical diode.

  6. Mechanical seal program

    International Nuclear Information System (INIS)

    Lowery, G.B.

    1983-01-01

    The experimental plans and timing for completion of the mechanical seal program for both the slurry and transfer pumps are given. The slurry pump seal program will be completed by April 1984 with turnover of two seals in pumps to SRP Tank 15H. Transfer pump seal design will be released for plant use by May 1984. Also included are various other pump and seal related tests

  7. A simple and sensitive HPLC method for analysis of imipramine in human plasma with UV detection and liquid-liquid extraction: Application in bioequivalence studies.

    Science.gov (United States)

    Rezazadeh, Mahboubeh; Emami, Jaber

    2016-01-01

    High-performance liquid chromatography (HPLC) methods employing ultraviolet (UV) detector are not sufficiently sensitive to measure the low plasma concentrations following single oral dose of imipramine. Therefore, in the present study a simple, rapid and yet sensitive HPLC method with UV detection was developed and validated for quantitation of imipramine in human plasma samples. An efficient liquid-liquid extraction (LLE) of imipramine from plasma with the mixture of hexane/isoamyl alcohol (98:2) and back extraction of the drug in acidic medium concomitant with evaporation of organic phase allowed the use of UV detector to conveniently measure plasma levels of this compound as low level as 3 ng/ml. Separation was achieved on a μ-Bondapak C18 HPLC column using sodium hydrogen phosphate solution (0.01 M)/acetonitrile (60/40 v/v) at pH 3.5 ± 0.1 at 1.5 ml/min. Trimipramine was used as the internal standard for analysis of plasma samples. The retention times for imipramine and trimipramine were 4.3 and 5.2 min, respectively. Calibration curve was linear in the range of 3-40 ng/ml using human plasma with the average extraction recovery of 85 ± 5%. Imipramine was found to be stable in plasma samples with no evidence of degradation during three freeze-thaw cycles and three months storage at -70°C. The current validated method was finally applied in bioequivalence studies of two different imipramine products according to a standard two-way crossover design with a two weeks washout period.

  8. A uv-sensitive Chinese hamster lung fibroblast cell line (V79/UC) with a possible defect in DNA polymerase activity is deficient in DNA repair

    International Nuclear Information System (INIS)

    Creissen, D.M.; Hill, C.K.

    1991-01-01

    Studies of repair enzyme activities in a uv-sensitive cell line (V79/UC) derived from Chinese hamster V79 cells have revealed levels of total DNA polymerase that are about 50% of the levels in the parental cell line. There are a number of DNA polymerase inhibitors available which allow us to distinguish between the major forms of DNA polymerase (alpha, beta, gamma, and delta) identified in mammalian cells. Enzyme assays with these inhibitors indicate that the aphidicolin-sensitive DNA polymerase is defective in the V79/UC cell line. This could be either polymerase alpha or delta, or both. The V79/UC cells do not express resistance to aphidicolin in standard toxicity studies. However, when aphidicolin is added postirradiation in survival assays designed to measure the extent of inhibitable repair, V79/UC cells do not respond with the further decrease in survival seen in the parental line. Further evidence of a polymerase-dependent repair defect is evident from alkaline elution data. In this case the V79/UC cells show the appearance of single-strand breaks following uv irradiation in the absence of any added inhibitor. Cells of the V79/M12G parental line, on the other hand, show the appearance of single-strand breaks only when aphidicolin is present

  9. The feasibility of retrieving vertical temperature profiles from satellite nadir UV observations: A sensitivity analysis and an inversion experiment with neural network algorithms

    International Nuclear Information System (INIS)

    Sellitto, P.; Del Frate, F.

    2014-01-01

    Atmospheric temperature profiles are inferred from passive satellite instruments, using thermal infrared or microwave observations. Here we investigate on the feasibility of the retrieval of height resolved temperature information in the ultraviolet spectral region. The temperature dependence of the absorption cross sections of ozone in the Huggins band, in particular in the interval 320–325 nm, is exploited. We carried out a sensitivity analysis and demonstrated that a non-negligible information on the temperature profile can be extracted from this small band. Starting from these results, we developed a neural network inversion algorithm, trained and tested with simulated nadir EnviSat-SCIAMACHY ultraviolet observations. The algorithm is able to retrieve the temperature profile with root mean square errors and biases comparable to existing retrieval schemes that use thermal infrared or microwave observations. This demonstrates, for the first time, the feasibility of temperature profiles retrieval from space-borne instruments operating in the ultraviolet. - Highlights: • A sensitivity analysis and an inversion scheme to retrieve temperature profiles from satellite UV observations (320–325 nm). • The exploitation of the temperature dependence of the absorption cross section of ozone in the Huggins band is proposed. • First demonstration of the feasibility of temperature profiles retrieval from satellite UV observations. • RMSEs and biases comparable with more established techniques involving TIR and MW observations

  10. Nuclear reactor sealing system

    International Nuclear Information System (INIS)

    McEdwards, J.A.

    1983-01-01

    A liquid metal-cooled nuclear reactor sealing system is disclosed. The nuclear reactor includes a vessel sealed at its upper end by a closure head. The closure head comprises at least two components, one of which is rotatable; and the two components define an annulus therebetween. The sealing system includes at least a first and second inflatable seal disposed in series in an upper portion of the annulus. The system further includes a dip seal extending into a body of insulation located adjacent a bottom portion of the closure head. The dip seal comprises a trough formed by a lower portion of one of the components, and a seal blade pendently supported from the other component and extending downwardly into the trough. A body of liquid metal is contained in the trough which submerges a portion of the seal blade. The seal blade is provided with at least one aperture located above the body of liquid metal for providing fluid communication between the annulus intermediate the dip seal and the inflatable seals, and a body of cover gas located inside the vessel. There also is provided means for introducing a purge gas into the annulus intermediate the inflatable seals and the seal blade. The purge gas is introduced in an amount sufficient to substantially reduce diffusion of radioactive cover gas or sodium vapor up to the inflatable seals. The purge gas mixes with the cover gas in the reactor vessel where it can be withdrawn from the vessel for treatment and recycle to the vessel

  11. Inboard seal mounting

    Science.gov (United States)

    Hayes, John R. (Inventor)

    1983-01-01

    A regenerator assembly for a gas turbine engine has a hot side seal assembly formed in part by a cast metal engine block having a seal recess formed therein that is configured to supportingly receive ceramic support blocks including an inboard face thereon having a regenerator seal face bonded thereto. A pressurized leaf seal is interposed between the ceramic support block and the cast metal engine block to bias the seal wear face into sealing engagement with a hot side surface of a rotary regenerator matrix.

  12. High-sensitivity detection of cardiac troponin I with UV LED excitation for use in point-of-care immunoassay

    OpenAIRE

    Rodenko, Olga; Eriksson, Susann; Tidemand-Lichtenberg, Peter; Troldborg, Carl Peder; Fodgaard, Henrik; van Os, Sylvana; Pedersen, Christian

    2017-01-01

    High-sensitivity cardiac troponin assay development enables determination of biological variation in healthy populations, more accurate interpretation of clinical results and points towards earlier diagnosis and rule-out of acute myocardial infarction. In this paper, we report on preliminary tests of an immunoassay analyzer employing an optimized LED excitation to measure on a standard troponin I and a novel research high-sensitivity troponin I assay. The limit of detection is improved by fac...

  13. Acoustic resonance spectroscopy intrinsic seals

    International Nuclear Information System (INIS)

    Olinger, C.T.; Burr, T.; Vnuk, D.R.

    1994-01-01

    We have begun to quantify the ability of acoustic resonance spectroscopy (ARS) to detect the removal and replacement of the lid of a simulated special nuclear materials drum. Conceptually, the acoustic spectrum of a container establishcs a baseline fingerprint, which we refer to as an intrinsic seal, for the container. Simply removing and replacing the lid changes some of the resonant frequencies because it is impossible to exactly duplicate all of the stress patterns between the lid and container. Preliminary qualitative results suggested that the ARS intrinsic seal could discriminate between cases where a lid has or has not been removed. The present work is directed at quantifying the utility of the ARS intrinsic seal technique, including the technique's sensitivity to ''nuisance'' effects, such as temperature swings, movement of the container, and placement of the transducers. These early quantitative tests support the potential of the ARS intrinsic seal application, but also reveal a possible sensitivity to nuisance effects that could limit environments or conditions under which the technique is effective

  14. Nuclear waste vault sealing

    International Nuclear Information System (INIS)

    Gyenge, M.

    1980-01-01

    A nuclear waste vault must be designed and built to ensure adequate isolation of the nuclear wastes from human contact. Consequently, after a vault has been fully loaded, it must be adequately sealed off to prevent radionuclide migration which may be provided by circulating groundwater. Vault sealing entails four major aspects, i.e.: (a) vault grouting; (b) borehole sealing; (c) buffer packing; and (d) backfilling. Of particular concern in vault sealing are the physical and chemical properties of the sealing material, its long-term durability and stability, and the techniques used for its emplacement. Present sealing technology and sealing materials are reviewed in terms of the particular needs of vault sealing. Areas requiring research and development are indicated

  15. Reactor vessel sealing plug

    International Nuclear Information System (INIS)

    Dooley, R.A.

    1986-01-01

    This invention relates to an apparatus and method for sealing the cold leg nozzles of a nuclear reactor pressure vessel from a remote location during maintenance and inspection of associated steam generators and pumps while the pressure vessel and refueling canal are filled with water. The apparatus includes a sealing plug for mechanically sealing the cold leg nozzle from the inside of a reactor pressure vessel. The sealing plugs include a primary and a secondary O-ring. An installation tool is suspended within the reactor vessel and carries the sealing plug. The tool telescopes to insert the sealing plug within the cold leg nozzle, and to subsequently remove the plug. Hydraulic means are used to activate the sealing plug, and support means serve to suspend the installation tool within the reactor vessel during installation and removal of the sealing plug

  16. Fog seal guidelines.

    Science.gov (United States)

    2003-10-01

    Fog seals are a method of adding asphalt to an existing pavement surface to improve sealing or waterproofing, prevent further stone loss by holding aggregate in place, or simply improve the surface appearance. However, inappropriate use can result in...

  17. High-sensitivity detection of cardiac troponin I with UV LED excitation for use in point-of-care immunoassay

    DEFF Research Database (Denmark)

    Rodenko, Olga; Eriksson, Susann; Tidemand-Lichtenberg, Peter

    2017-01-01

    of an immunoassay analyzer employing an optimized LED excitation to measure on a standard troponin I and a novel research high-sensitivity troponin I assay. The limit of detection is improved by factor of 5 for standard troponin I and by factor of 3 for a research high-sensitivity troponin I assay, compared...... to the flash lamp excitation. The obtained limit of detection was 0.22 ng/L measured on plasma with the research highsensitivity troponin I assay and 1.9 ng/L measured on tris-saline-azide buffer containing bovine serum albumin with the standard troponin I assay. We discuss the optimization of time...

  18. Action of cis-dichlorobis (cyclopentylamine) platinum (2) (cis-PAD) on L5178Y cells of two strains inversely cross-sensitive to X-rays and UV-light. Part 3. Differential effects of caffeine

    International Nuclear Information System (INIS)

    Szumiel, I.

    1977-01-01

    Two strains of L5178Y murine lymphoma, inversely cross-sensitive to X-rays and UV-light, were shown previously to respond to treatment with an antitumour platinum complex, cis-PAD, in a similar manner as to UV. The difference in sensitivity to cis-PAD, found in L5178Y-R and L5178Y-S cells is not caused by a difference in ability to bind platinum complex. Enhancement of chromosomal damage and potentiation of lethal effect of cis-PAD by 0.75 mM caffeine were found in cis-PAD and UV-light-resistant L5178Y-S strain but not in cis-PAD and UV-light-sensitive L5178Y-R strain. These results suggest that the extreme sensitivity of L5178Y-R strain to cis-PAD and UV-light is caused to some extent by deficiency in a caffeine-sensitive post-replication repair system. (author)

  19. Hermetically Sealed Compressor

    Science.gov (United States)

    Holtzapple, Mark T.

    1994-01-01

    Proposed hermetically sealed pump compresses fluid to pressure up to 4,000 atm (400 MPa). Pump employs linear electric motor instead of rotary motor to avoid need for leakage-prone rotary seals. In addition, linear-motor-powered pump would not require packings to seal its piston. Concept thus eliminates major cause of friction and wear. Pump is double-ended diaphragm-type compressor. All moving parts sealed within compressor housing.

  20. Design of Matched Cladding Fiber with UV-sensitive Cladding for Minimization of Claddingmode Losses in Fiber Bragg Gratings

    DEFF Research Database (Denmark)

    Nielsen, Mads Lønstrup; Berendt, Martin Ole; Bjarklev, Anders Overgaard

    2000-01-01

    The effect on the Bragg-grating-induced cladding-mode coupling of varying the extent of the photosensitive region in a step-index fiber is analyzed. We introduce a figure of merit for the suppression of cladding-mode loss and compare different matched cladding fiber designs. It is found to be adv......The effect on the Bragg-grating-induced cladding-mode coupling of varying the extent of the photosensitive region in a step-index fiber is analyzed. We introduce a figure of merit for the suppression of cladding-mode loss and compare different matched cladding fiber designs. It is found...... to be advantageous to increase the extent of the photosensitive region. However, no significant improvement is obtained by extending the photosensitive region more than approximately 10 mu m into the cladding. This result is not in agreement with a simple analysis that neglects UV absorption, which suggests...... that the radius of the photosensitive region should be close to twice as large. (C) 2000 Academic Press....

  1. Increased sensitivity of UV-repair-deficient human cells to DNA bound platinum products which unlike thymine dimers are not recognised by an endonuclease extracted from Micrococcus luteus

    Energy Technology Data Exchange (ETDEWEB)

    Fraval, H N.A.; Rawlings, C J; Roberts, J J [Institute of Cancer Research, Royal Cancer Hospital, Pollards Wood Research Station, Chalfont St. Giles, Bucks, UK

    1978-07-01

    The response of human cells in culture to cis platinum (II) diammine dichloride (cis Pt(II)) induced DNA damage has been studied. The survival data, measured as a function of cis Pt(II) dose were similar in a normal cell line (Human foetal lung) compared to a UV-sensitive, thymine dimer excision repair-deficient cell line (Xeroderma pigmentatosum). However, there was a marked difference between the two cell lines when binding to DNA was plotted against dose of cis Pt(II) given for 1 h. When these findings were expressed as cell survival versus binding to DNA, a 4.1-fold difference between the slopes of the survival curves for the two cell lines was obtained. These findings are consistent with the notion that normal cells are able to excise cis Pt(II) induced damage from their genome and thus increase their ability to survive as compared to excision deficient cells. An endonuclease preparation from Micrococcus luteus is able to recognise UV damage in DNA, but did not recognise cis Pt(II) induced damage. These results possibly indicate differences in the pathways of repair of damage caused by the two agents.

  2. High-sensitivity detection of cardiac troponin I with UV LED excitation for use in point-of-care immunoassay.

    Science.gov (United States)

    Rodenko, Olga; Eriksson, Susann; Tidemand-Lichtenberg, Peter; Troldborg, Carl Peder; Fodgaard, Henrik; van Os, Sylvana; Pedersen, Christian

    2017-08-01

    High-sensitivity cardiac troponin assay development enables determination of biological variation in healthy populations, more accurate interpretation of clinical results and points towards earlier diagnosis and rule-out of acute myocardial infarction. In this paper, we report on preliminary tests of an immunoassay analyzer employing an optimized LED excitation to measure on a standard troponin I and a novel research high-sensitivity troponin I assay. The limit of detection is improved by factor of 5 for standard troponin I and by factor of 3 for a research high-sensitivity troponin I assay, compared to the flash lamp excitation. The obtained limit of detection was 0.22 ng/L measured on plasma with the research high-sensitivity troponin I assay and 1.9 ng/L measured on tris-saline-azide buffer containing bovine serum albumin with the standard troponin I assay. We discuss the optimization of time-resolved detection of lanthanide fluorescence based on the time constants of the system and analyze the background and noise sources in a heterogeneous fluoroimmunoassay. We determine the limiting factors and their impact on the measurement performance. The suggested model can be generally applied to fluoroimmunoassays employing the dry-cup concept.

  3. Investigation of positive shaft seals

    Science.gov (United States)

    Pfouts, J. O.

    1970-01-01

    Welded metal bellows secondary seals prevent secondary seal leakage with a minimum number of potential leak paths. High performance seal is obtained by controlling the potentially unstable seal-face movements induced by mechanical vibrations and fluid pressure pulsations.

  4. Circumferential shaft seal

    Science.gov (United States)

    Ludwig, L. P. (Inventor)

    1981-01-01

    A circumferential shaft seal comprising two sealing rings held to a rotating shaft by means of a surrounding elastomeric band is disclosed. The rings are segmented and are of a rigid sealing material such as carbon or a polyimide and graphite fiber composite.

  5. Seals in motion

    NARCIS (Netherlands)

    Brasseur, Sophie Marie Jacqueline Michelle

    2017-01-01

    The harbour seal Phoca vitulina and the grey seal Halichoerus grypus have been inhabitants of the Wadden Sea since millennia. Prehistoric findings indicate the presence of both species around 5000 BC. This changed dramatically in the mid Middle-Ages as around 1500 AC, the grey seal disappeared from

  6. Wafer-scaled monolayer WO{sub 3} windows ultra-sensitive, extremely-fast and stable UV-A photodetection

    Energy Technology Data Exchange (ETDEWEB)

    Hai, Zhenyin; Akbari, Mohammad Karbalaei [Ghent University Global Campus, Department of Applied Analytical & Physical Chemistry, Faculty of Bioscience Engineering, 119 Songdomunhwa-ro, Yeonsu-gu, Incheon 21985 (Korea, Republic of); Xue, Chenyang [Key Laboratory of Instrumentation Science and Dynamic Measurement of Ministry of Education, North University of China, Taiyuan, Shanxi 030051 (China); Xu, Hongyan [School of Materials Science and Engineering, North University of China, Taiyuan, Shanxi 030051 (China); Hyde, Lachlan [Melbourne Centre for Nanofabrication, Clayton, Victoria 3168 (Australia); Zhuiykov, Serge, E-mail: serge.zhuiykov@ugent.be [Ghent University Global Campus, Department of Applied Analytical & Physical Chemistry, Faculty of Bioscience Engineering, 119 Songdomunhwa-ro, Yeonsu-gu, Incheon 21985 (Korea, Republic of)

    2017-05-31

    Highlights: • Monolayer WO{sub 3}-based photodetectors were fabricated for the first time. • The device has ultrafast response time of ∼40 μs and responsivity of ∼0.329 A W{sup −1}. • The response time is 400-fold improvement over any other WO{sub 3} UV photodetectors. • The device has better characteristics than many 2D materials-based photodetectors. • This proposed strategy has great potential for commercialization of photodetectors. - Abstract: The monolayer WO{sub 3}-based UV-A photodetectors, fabricated by atomic layer deposition (ALD) technique at the large area of SiO{sub 2}/Si wafer, have demonstrated vastly improved functional capabilities: extremely fast response time of less than 40 μs and photoresponsivity reaching of ∼0.329 A W{sup −1}. Their ultrafast photoresponse time is at least 400-fold improvement over the previous reports for any other WO{sub 3}-based UV photodetectors that have ever been fabricated, and significantly faster than most of other photodetectors based on two-dimensional (2D) nanomaterials reported-to-date. Moreover, their measured long-term stability exceeds more than 200 cycles without any visible degradation. The ALD-deposited WO{sub 3} monolayer has also exhibited wider bandgap of 3.53 eV and the UV-A photodetector based on it is environmentally friendly, highly reliable, with excellent reproducibility and long-term stability. Thus, the shift to mono-layered semiconductors, which possess completely new quantum-confined effects, has the greatest potential in creating a new class of nano-materials, which in return windows new functional opportunities for various opto-electronic instruments built on semiconductor monolayer and, more importantly, can result in new strategy for fabrication highly-flexible, inexpensive and extremely-sensitive devices. This strategy also opens up the great opportunities for industrialization and commercialization of the photodetectors and other optoelectronic devices based on

  7. Functional characterization of spectral tuning mechanisms in the great bowerbird short-wavelength sensitive visual pigment (SWS1), and the origins of UV/violet vision in passerines and parrots.

    Science.gov (United States)

    van Hazel, Ilke; Sabouhanian, Amir; Day, Lainy; Endler, John A; Chang, Belinda S W

    2013-11-13

    One of the most striking features of avian vision is the variation in spectral sensitivity of the short wavelength sensitive (SWS1) opsins, which can be divided into two sub-types: violet- and UV- sensitive (VS & UVS). In birds, UVS has been found in both passerines and parrots, groups that were recently shown to be sister orders. While all parrots are thought to be UVS, recent evidence suggests some passerine lineages may also be VS. The great bowerbird (Chlamydera nuchalis) is a passerine notable for its courtship behaviours in which males build and decorate elaborate bower structures. The great bowerbird SWS1 sequence possesses an unusual residue combination at known spectral tuning sites that has not been previously investigated in mutagenesis experiments. In this study, the SWS1 opsin of C. nuchalis was expressed along with a series of spectral tuning mutants and ancestral passerine SWS1 pigments, allowing us to investigate spectral tuning mechanisms and explore the evolution of UV/violet sensitivity in early passerines and parrots. The expressed C. nuchalis SWS1 opsin was found to be a VS pigment, with a λmax of 403 nm. Bowerbird SWS1 mutants C86F, S90C, and C86S/S90C all shifted λmax into the UV, whereas C86S had no effect. Experimentally recreated ancestral passerine and parrot/passerine SWS1 pigments were both found to be VS, indicating that UV sensitivity evolved independently in passerines and parrots from a VS ancestor. Our mutagenesis studies indicate that spectral tuning in C. nuchalis is mediated by mechanisms similar to those of other birds. Interestingly, our ancestral sequence reconstructions of SWS1 in landbird evolution suggest multiple transitions from VS to UVS, but no instances of the reverse. Our results not only provide a more precise prediction of where these spectral sensitivity shifts occurred, but also confirm the hypothesis that birds are an unusual exception among vertebrates where some descendants re-evolved UVS from a violet type

  8. Two models of distribution of sites sensitive to the endonuclease from Micrococcus luteus in the DNA of UV irradiated Escherichia coli B/r Hcr-

    International Nuclear Information System (INIS)

    Kleibl, K.; Sedliakova, M.

    1984-01-01

    Cells prelabelled with 14 C-thymine and irradiated with 5 J/m 2 were at various intervals after UV labelled with 3 H-thymidine then treated with the extract from M. luteus and DNA was analyzed in alkaline sucrose gradients. Loss of endonuclease sensitive sites (Es sites) from the parental DNA and their occurrence in the daughter DNA were followed for at least three replication cycles. Data obtained indicate that about 50% of Es sites were lost during the first replication cycle but no additional loss was observed during subsequent cycles. Thus our data do not support a hypothesis that a half of the dimers are transferred from the parental into the daughter strands at each replication cycle. They rather indicate that dimers remain in situ and distortions accompanying dimers are distinguished either on the side of the parental or on the side of the daughter strands with an equal probability. (author)

  9. Electronic self-monitoring seal

    International Nuclear Information System (INIS)

    Campbell, J.W.

    1978-01-01

    The Electronic Self-Monitoring Seal is a new type of security seal which allows continuous verification of the seal's identity and status. The identity information is a function of the individual seal, time, and seal integrity. A description of this seal and its characteristics are presented. Also described are the use cycle for the seal and the support equipment for programming and verifying the seal

  10. Tamper tape seals

    International Nuclear Information System (INIS)

    Wright, B.W.; Undem, H.A.

    1994-07-01

    Tamper tapes are appealing for many applications due to their ease of use and relative robustness. Applications include seals for temporary area denial, protection of sensitive equipment, chain-of-custody audit trails, and inventory control practices. A next generation of adhesive tamper tapes is being developed that combines the best features of commercially available devices with additional state-of-the-art features in tamper indication, tamper-resistance, and counterfeit-resistance. The additional features are based on U.S. Department of Energy (DOE) research and development (R ampersand D) activities that were originally associated with preparations for the Strategic Arms Reduction Treaty (START). New features include rapid-set, chemical-cure adhesive systems that allow user-friendly application and layered levels of counterfeit-resistance based on unique open-quotes fingerprintclose quotes characteristics that can be accessed as desired

  11. Sensitization by UV light of α-Al{sub 2}O{sub 3}:C polycrystalline detectors

    Energy Technology Data Exchange (ETDEWEB)

    Meira B, L. C.; Rubio F, H.; Neres de A, E. [Centro de Desenvolvimento da Tecnologia Nuclear - CNEN, Laboratorio de Dosimetria Termoluminicente, Av. Antonio Carlos 6627, Campus UFMG, CEP 31270-901, Belo Horizonte (Brazil); Santos, A., E-mail: hrf@cdtn.br [Centro de Desenvolvimento da Tecnologia Nuclear - CNEN, Laboratorio de Microesferas Gel, Av. Antonio Carlos 6627, Campus UFMG, CEP 31270-901, Belo Horizonte (Brazil)

    2014-08-15

    This paper describes an increase in sensitivity to gamma and beta radiation on α-Al{sub 2}O{sub 3}:C polycrystalline detector, which has been produced by a sol-gel process, following previous exposure to ultraviolet light. The increased sensitivity of the detector as a function of the exposure time and ultraviolet wavelength was studied. Since the main luminescent centers have emission peaks at different wavelengths, selective measurements of thermoluminescent emission intensity were done, in order to investigate the possible conversion of centers as a result of the exposition to ultraviolet light. Experimental results indicate that the nature and parameters of the luminescent centers in α-Al{sub 2}O{sub 3}:C sol-gel material can be very different of those in α-Al{sub 2}O{sub 3}:C single crystal. (author)

  12. VAK III. Seals and sealing system

    International Nuclear Information System (INIS)

    d'Agraives, B.C.; Dal Cero, G.; Debeir, R.; Mascetti, E.; Toornvliet, J.; Volcan, A.

    1986-01-01

    This report presents the VAK III seals and sealing system, which have been used over a period of two years at the Kahl nuclear facility (Federal Republic of Germany), where field tests and feasibility studies were conducted in order to offer a possible solution for the sealing of LWR fuel assemblies. It has been prepared with the aim of an assessment study to be done at the IAEA. It gives all characteristics and technical descriptions for: the sealing principle, the seal construction, the operating tools, the data processing, the drawings, the publications related to that seal. The main points of progress are: the Strong Random Internal Defects (STRID) incorporated in the seals, allowing the obtention of a good signature stability; the Integrity Check on the Seal Status (broken or not) obtained through a decisive mechanical improvement: the Double Breakage Integrity Check (DOBRIC) and with a better ultrasonic evidence of that status; the provision of new function tools, allowing the performance of Identity Measurements in dry conditions (which means also at the manufacturer plant) or in deeper water (wet storage); the study and development of a new JRC VAK 45 Compact Instrument Box, in which all the measuring functions can be grouped and incorporating an autonomous Minicomputer offering to the Inspection the possibility of performing, on the spot, Correlation and Decision processes. The general benefit of such a feasibility study should be to convince the potential users that such a Safeguards Sealing System can be studied for slightly - or largely - different other applications, provided that the Basic and Operating Functions required to the system be clearly defined, possibly after a common agreement would be stated

  13. Reinforced seal component

    International Nuclear Information System (INIS)

    Jeanson, G.M.; Odent, R.P.

    1980-01-01

    The invention concerns a seal component of the kind comprising a soft sheath and a flexible reinforcement housed throughout the entire length of the sheath. The invention enables O ring seals to be made capable of providing a radial seal, that is to say between two sides or flat collars of two cylindrical mechanical parts, or an axial seal, that is to say between two co-axial axisymmetrical areas. The seal so ensured is relative, but it remains adequately sufficient for many uses, for instance, to ensure the separation of two successive fixed blading compartments of axial compressors used in gas diffusion isotope concentration facilities [fr

  14. The IRES electronic seal

    International Nuclear Information System (INIS)

    Autrusson, B.; Brochard, D.; Moreau, J.F.; Martin, J.C.

    2001-01-01

    In the framework of the French Support Program for the IAEA Safeguards, the 'Institut de Protection et de Surete Nucleaire' (IPSN), developed an electronic seal called Integrated and Reusable Electronic Seal (IRES) that enables independent verification by different inspectorates (IAEA, Euratom, and National Inspectorate). The seal can be remotely interrogated by radio frequency and integrated to other Containment/surveillance systems by serial line RS 485. Data are authenticated and the IRESMAG software manages in the seal reader all functionalities of the seal and records inspection data compatible with the IAEA's Seal Database. To perform this development, IPSN relies on industrial partners: SAPHYMO for the general architecture of the seal and the electronics, THALES for the authentication of data and the security of transmission. The main features of the IRES seal are the following: Interrogation by different inspectorate, allowing independent conclusions; Recording of events, including tampering, in a non-volatile memory; Authentication of data and enhanced security of the communication between the seal and the seal reader; Remote interrogation by an inspector or/and automatic for unattended systems or remote monitoring; Reusable after erasing the seal memory and replacement of the batteries

  15. Severe service sealing solutions

    International Nuclear Information System (INIS)

    Metcalfe, R.; Wensel, R.

    1994-09-01

    Successful sealing usually requires much more than initial leak-tightness. Friction and wear must also be acceptable, requiring a good understanding of tribology at the sealing interface. This paper describes various sealing solutions for severe service conditions. The CAN2A and CAN8 rotary face seals use tungsten carbide against carbon-graphite to achieve low leakage and long lifetime in nuclear main coolant pumps. The smaller CAN6 seal successfully uses tungsten carbide against silicon carbide in reactor water cleanup pump service. Where friction in CANDU fuelling machine rams must be essentially zero, a hydrostatic seal using two silicon carbide faces is the solution. In the NRU reactor moderator pumps, where pressure is much lower, eccentric seals that prevent boiling at the seal faces are giving excellent service. All these rotary face seals rely on supplementary elastomer seals between their parts. An integrated engineering approach to high performance sealing with O-rings is described. This is epitomized in critical Space Shuttle applications, but is increasingly being applied in CANDU plants. It includes gland design, selection and qualification of material, quality assurance, detection of defects and the effects of lubrication, surface finish, squeeze, stretch and volume constraints. In conclusion, for the severe service applications described, customized solutions have more than paid for themselves by higher reliability, lower maintenance requirements and reduced outage time. (author)

  16. The IRES electronic seal

    International Nuclear Information System (INIS)

    Gourlez, P.; Funk, P.; Brochard, D.; Moreau, J.F.; Martin, J.C.

    2001-01-01

    In the framework of the French Support Program for the IAEA Safeguards, the 'Institut de Protection et de Surete Nucleaire' (IPSN), developed an electronic seal called Integrated and Reusable Electronic Seal (IRES) that enables independent verification by different inspectorates (IAEA, Euratom, and National Inspectorate) Furthermore, a bilateral co-ordination between Euratom and French domestic safeguards takes place in some French facilities regarding a common approach concerning the seals especially in case of crisis situation. The seal can be remotely interrogated by radio frequency and integrated to other Containment/surveillance systems by serial line RS 485. Data are authenticated and the IRESMAG software manages in the seal reader all functionalities of the seal and records inspection data compatible with the IAEA's Seal Database

  17. High pressure shaft seal

    International Nuclear Information System (INIS)

    Martinson, A.R.; Rogers, V.D.

    1980-01-01

    In relation to reactor primary coolant pumps, mechanical seal assembly for a pump shaft is disclosed which features a rotating seal ring mounting system which utilizes a rigid support ring loaded through narrow annular projections in combination with centering non-sealing O-rings which effectively isolate the rotating seal ring from temperature and pressure transients while securely positioning the ring to adjacent parts. A stationary seal ring mounting configuration allows the stationary seal ring freedom of motion to follow shaft axial movement up to 3/4 of an inch and shaft tilt about the pump axis without any change in the hydraulic or pressure loading on the stationary seal ring or its carrier. (author)

  18. TL sensitivity constancy of quartz upon UV+(β,γ) irradiation cycle: An improvement on dating methodology

    International Nuclear Information System (INIS)

    Souza, J.H.

    1985-01-01

    Thermoluminescence (TL) sensivitivy of natural quartz (beaches, paleobeaches and fixed dunes) to Beta and Gamma rays has been studied in a temperature range between 250-400 0 C, before and after bleaching by solar irradiation. A first TL glow growth curve was obtained through Beta irradiation of 'as found' samples and a second glow growth curve was obtained by bleaching through solar irradiation the TL acquired either through an excitation Gamma dose or through environmental radiation to finally re-irradiate the samples under Beta rays. Experimental results showing the constancy of sensitivity, for doses of until about 10K rads, are the basis for a proposal to improve TL dating methodology expanding its present limits. (Author) [pt

  19. Flexible ring seal

    International Nuclear Information System (INIS)

    Abbes, Claude; Gournier, Andre; Rouaud, Christian; Villepoix, Raymond de.

    1976-01-01

    The invention concerns a flexible metal ring seal, able to ensure a perfect seal between two bearings due to the crushing and elastic deformation properties akin to similar properties in elastomers. Various designs of seal of this kind are already known, particularly a seal made of a core formed by a helical wire spring with close-wound turns and with high axial compression ratio, closed on itself and having the shape of an annulus. This wire ring is surrounded by at least one envelope having at rest the shape of a toroidal surface of which the generating circle does not close on itself. In a particular design mode, the seal in question can include, around the internal spring, two envelopes of which one in contact with the spring is composed of a low ductility elastic metal, such as mild steel or stainless steel and the other is, on the contrary, made of a malleable metal, such as copper or nickel. The first envelope evenly distributes the partial crushing of the spring, when the seal is tightened, on the second envelope which closely fits the two surfaces between which the seal operates. The stress-crushing curve characteristic of the seal comprises two separate parts, the first with a relatively sharp slope corresponds to the start of the seal compression phase, enabling at least some of these curves to reach the requisite seal threshold very quickly, then, beyond this, a second part, practically flat, where the stress is appreciably constant for a wide operating bracket [fr

  20. Near-UV sensitized 1.06 μm emission of Nd{sup 3+} ions via monovalent copper in phosphate glass

    Energy Technology Data Exchange (ETDEWEB)

    Jiménez, José A., E-mail: jose.jimenez@unf.edu [Department of Chemistry, University of North Florida, Jacksonville, FL 32224 (United States); Sendova, Mariana [Optical Spectroscopy & Nano-Materials Lab, New College of Florida, Sarasota, FL 34243 (United States)

    2015-07-15

    Monovalent copper ions effectively incorporated in Nd-containing phosphate glass by a single-step melt-quench method have been established as near-ultraviolet (UV) sensitizers of Nd{sup 3+} ions, resulting in a remarkable {sup 4}F{sub 3/2} → {sup 4}I{sub 11/2} emission at 1.06 μm. The spectroscopic data indicates an efficient energy conversion process. The Cu{sup +} ions first absorb photons broadly around 360 nm, and subsequently transfer the energy from the Stokes-shifted emitting states to resonant Nd{sup 3+} energy levels in the visible. Ultimately, the Nd{sup 3+} electronic excited states decay and the upper lasing state {sup 4}F{sub 3/2} is populated, leading to the enhanced emission at 1.06 μm. The characteristic features of the Cu{sup +} visible emission spectra and the reduced lifetime of the corresponding Cu{sup +} donor states indicate an efficient non-radiative transfer. The Cu{sup +}/Nd{sup 3+} co-doped phosphate glass appears suitable as solid-state laser material with enhanced pump range in the near-UV part of the spectrum and for solar spectral conversion in photovoltaic cells. - Graphical abstract: Display Omitted - Highlights: • Monovalent copper ions effectively stabilized in Nd{sup 3+}-containing phosphate glass. • Enhanced Nd{sup 3+} near-infrared emission observed upon the Cu{sup +} ions incorporation. • Cu{sup +} → Nd{sup 3+} non-radiative energy transfer efficiencies and likely energy transfer pathways evaluated. • Potential for solid-state lasers and solar spectral conversion suggested.

  1. Solvent-assisted self-assembly of fullerene into single-crystal ultrathin microribbons as highly sensitive UV-visible photodetectors.

    Science.gov (United States)

    Wei, Lang; Yao, Jiannian; Fu, Hongbing

    2013-09-24

    The size, shape, and crystallinity of organic nanostructures play an important role in their physical properties and are mainly determined by the self-assembling kinetics of molecular components often involving the solvent conditions. Here, we reported a kinetically controlled self-assembly of C60 assisted by the solvent carbon bisulfide (CS2) into single-crystal ultrathin microribbons of 2C60·3CS2, upon mixing the poor solvent isopropyl alcohol with a C60/CS2 stock solution. Surface energy calculations reveal that these microribbons represent a kinetically favored high-energy state as compared with the thermodynamically stable shape of prismatic rods. High-resolution transmission electron microscopy observations clarify that association of CS2 at the nucleation stage helps to guide and rigidify the formation of π-π stacking 1D chains of C60 through the surrounding CS2 cage-like structures, which further act as glue, boosting lateral assembly of as-formed 1D chains into untrathin 2D microribbon single crystals. Precise control over the thickness, width, and length of 2C60·3CS2 microribbons was achieved by manipulation of the growth kinetics through adjusting the solvent conditions. Upon heating to 120 °C, sublimation of CS2 components results in fcc C60 microribbons. We found that both microribbons of solvated monoclinic 2C60·3CS2 and pure fcc C60 exhibit highly sensitive photoconductivity properties with a spectral response range covering UV to visible. The highest on/off ratio of two-terminal photodetectors based on single ribbons reaches around 250, while the responsitivity is about 75.3 A W(-1) in the UV region and 90.4 A W(-1) in the visible region.

  2. Self-assembly of nitrogen-doped carbon nanoparticles: a new ratiometric UV-vis optical sensor for the highly sensitive and selective detection of Hg(2+) in aqueous solution.

    Science.gov (United States)

    Ruan, Yudi; Wu, Lie; Jiang, Xiue

    2016-05-23

    Water-soluble nitrogen-doped carbon nanoparticles (N-CNPs) prepared by the one-step hydrothermal treatment of uric acid were found to show ratiometric changes in their UV-vis spectra due to Hg(2+)-mediated self-assembly. For the first time, such a property was developed into a UV-vis optical sensor for detecting Hg(2+) in aqueous solutions with high sensitively and selectively (detection limit = 1.4 nM). More importantly, this novel sensor exhibits a higher linear sensitivity over a wider concentration range compared with the fluorescence sensor based on the same N-CNPs. This work opens an exciting new avenue to explore the use of carbon nanoparticles in constructing UV-vis optical sensors for the detection of metal ions and the use of carbon nanoparticles as a new building block to self-assemble into superlattices.

  3. The Effect of UV-Irradiation (under Short-Circuit Condition) on Dye-Sensitized Solar Cells Sensitized with a Ru-Complex Dye Functionalized with a (diphenylamino)Styryl-Thio phen Group

    International Nuclear Information System (INIS)

    Nonomura, K.; Xu, Y.; Marinado, T.; Hagberg, D.P.; Sun, L.; Boschloo, G.; Hagfeldt, A.; Rong Zhang, R.; Boschloo, G.; Hagfeldt, A.

    2009-01-01

    A new ruthenium complex, cis-di(thiocyanato)(2,2'-bipyridine-4,4'-dicarboxylic acid)(4,4'-bis (2-(5-(2-(4-diphenylaminophenyl)ethenyl) -thiophen-2-yl)ethenyl)-2,2'-bipyridine)ruthenium(II) (named E322) has been synthesized for use in dye-sensitized solar cells (DSCs). Higher extinction coefficient and a broader absorption compared to the standard Ru-dye, N719, were aimed. DSCs were fabricated with E322, and the efficiency was 0.12% initially. (4.06% for N719, as reference). The efficiency was enhanced to 1.83% by exposing the cell under simulated sunlight containing UV-irradiation at short-circuit condition. The reasons of this enhancement are (1) enhanceing electron injection from sensitizer to TiO 2 following a shift toward positive potentials of the conduction band of TiO 2 by the adsorption of protons or cations from the sensitizer, or from the redox electrolyte and (2) improving the regeneration reaction of the oxidized dye by the redox electrolyte by the dissolution of aggregated dye from the surface of TiO 2 following the treatment.

  4. Seals in nuclear reactors

    International Nuclear Information System (INIS)

    1979-01-01

    The seals described are for use in a nuclear reactor where there are fuel assemblies in a vessel, an inlet and an outlet for circulating a coolant in heat transfer relationship with the fuel assemblies and a closure head on the vessel in a tight fluid relationship. The closure head comprises rotatable plugs which have mechanical seals disposed in the annulus around each plug while allowing free rotation of the plug when the seal is not actuated. The seal is usually an elastomer or copper. A means of actuating the seal is attached for drawing it vertically into the annulus for sealing. When the reactor coolant is liquid sodium, contact with oxygen must be avoided and argon cover gas fills the space between the bottom of the closure head and the coolant liquid level and the annuli in the closure head. (U.K.)

  5. Opsin expression in Limulus eyes: a UV opsin is expressed in each eye type and co-expressed with a visible light-sensitive opsin in ventral larval eyes.

    Science.gov (United States)

    Battelle, Barbara-Anne; Kempler, Karen E; Harrison, Alexandra; Dugger, Donald R; Payne, Richard

    2014-09-01

    The eyes of the horseshoe crab, Limulus polyphemus, are a model for studies of visual function and the visual systems of euarthropods. Much is known about the structure and function of L. polyphemus photoreceptors, much less about their photopigments. Three visible-light-sensitive L. polyphemus opsins were characterized previously (LpOps1, 2 and 5). Here we characterize a UV opsin (LpUVOps1) that is expressed in all three types of L. polyphemus eyes. It is expressed in most photoreceptors in median ocelli, the only L. polyphemus eyes in which UV sensitivity was previously detected, and in the dendrite of eccentric cells in lateral compound eyes. Therefore, eccentric cells, previously thought to be non-photosensitive second-order neurons, may actually be UV-sensitive photoreceptors. LpUVOps1 is also expressed in small photoreceptors in L. polyphemus ventral larval eyes, and intracellular recordings from these photoreceptors confirm that LpUVOps1 is an active, UV-sensitive photopigment. These photoreceptors also express LpOps5, which we demonstrate is an active, long-wavelength-sensitive photopigment. Thus small photoreceptors in ventral larval eyes, and probably those of the other larval eyes, have dual sensitivity to UV and visible light. Interestingly, the spectral tuning of small ventral photoreceptors may change day to night, because the level of LpOps5 in their rhabdoms is lower during the day than during the night, whereas LpUVOps1 levels show no diurnal change. These and previous findings show that opsin co-expression and the differential regulation of co-expressed opsins in rhabdoms is a common feature of L. polyphemus photoreceptors. © 2014. Published by The Company of Biologists Ltd.

  6. Seals in nuclear reactors

    International Nuclear Information System (INIS)

    1979-01-01

    The aim of this invention is the provision of improved seals for reactor vessels in which fuel assemblies are located together with inlets and outlets for the circulation of a coolant. The object is to provide a seal arrangement for the rotatable plugs of nuclear reactor closure heads which has good sealing capacities over a wide gap during operation of the reactor but which also permits uninhibited rotation of the plugs for maintenance. (U.K.)

  7. Compliant seal development

    Science.gov (United States)

    Hendricks, Robert C.

    1993-10-01

    The compliant metallic seal combines the noncontact feature of the labyrinth seal, the low leakage of a mechanical seal, and the compliant nature of the brush seal. It consists of several thin metallic elements or leaves mounted within a ring which is press fit into the housing, and in form, sort of resembles a lip seal sections wiping the shaft. A second set of overlapping cover leaves are placed on top of the shaft riding leaves which reduces leakage and provides stiffness. The leaves can be straight or angle cut. The shaft riding fingers are designed with mismatched curvature to provide lift off similar to the Rayleigh lift pads in mechanical seals with leading edge clearances nearly twice those of the trailing edge as as shown by Fleming to be optimal for gas flows in convergent seal passages. Leading edge clearances range from 300 to 500 microinches. Balance pockets beneath the leaves provide fluid film feed to the 'Rayleigh lift' surface and the proper balance ratio (mechanical seal) when combined with the static pressure and film pressure. The leaves flex in the radial direction and accommodate thermomechanical behavior as well as axial motion and angular misalignment. In the static mode, there is a net closing force on the leaves. The seals were tested to 70 psi at speeds to 16,000 rpm or surface speeds to 330 fps and temperatures from ambient to 440 F. A slow cycle through the rig critical at 10,000 rpm induced a radial vibration response of 0.004 to 0.005 inch were accommodated by the seal. Preliminary performance data are encouraging demonstrating hydrodynamic liftoff and noncontacting operation at pressure and speeds typical of gas turbine engines. The leakage performance data are significantly better than commercial labyrinth and brush seals which should be expected as this design incorporates the features of the low leakage face or mechanical seal along with the flexibility of the brush configuration.

  8. Seal containment system

    International Nuclear Information System (INIS)

    Kugler, R.W.; Gerkey, K.S.; Kasner, W.H.

    1978-01-01

    An automated system for transporting nuclear fuel elements between fuel element assembly stations without contaminating the area outside the sealed assembly stations is described. The system comprises a plurality of assembly stations connected together by an elongated horizontal sealing mechanism and an automatic transport mechanism for transporting a nuclear fuel element in a horizontal attitude between the assembly stations while the open end of the fuel element extends through the sealing mechanism into the assembly station enclosure. The sealing mechanism allows the fuel element to be advanced by the transport mechanism while limiting the escape of radioactive particles from within the assembly station enclosure. 4 claims, 6 figures

  9. Metabolic reduction of phenylpropanoid compounds in primary leaves of rye (Secale cereale L.) leads to increased UV-B sensitivity of photosynthesis

    International Nuclear Information System (INIS)

    Reuber, S.; Leitsch, J.; Krause, G.H.; Weissenböck, G.

    1993-01-01

    The present study was undertaken in order to investigate the suitability of certain markers for UV plant response. In addition, we attempted to link the internal tissue distribution of specific UV-absorbing compounds to profiles of radiation gradients within intact primary rye leaves (Secale cereale L. cv. Kustro). Etiolated rye seedlings irradiated with low visible light (LL) and/or UV radiation were used to study enzyme activities of the two key enzymes, phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS), together with the tissue-specific accumulation of soluble phenylpropanoid products. Plants grown under relatively high visible light (HL) with or without supplementary UV-B radiation were used for further characterization. Apparent quantum yield and fluorescence quenching parameters were monitored to assess potential physiological changes due to UV-B exposure in HL-grown seedlings. A quartz fibreoptic microprobe was used to characterize the internal UV-B gradient of the leaf. The response of the phenylpropanoid metabolism to UV radiation was similar in primary leaves of both etiolated and HL-treated green plants. The epidermis-specific flavonoids together with CHS activity turned out to be suitable markers for assessing the effect of UV on the phenolic metabolism. The functional role of phenylpropanoid compounds was strongly implicated in protecting rye from UV-B radiation

  10. Simultaneous resolution of spectral and temporal properties of UV and visible fluorescence using single-photon counting with a position-sensitive detector

    International Nuclear Information System (INIS)

    Kelly, L.A.; Trunk, J.G.; Polewski, K.; Sutherland, J.C.

    1995-01-01

    A new fluorescence spectrometer has been assembled at the U9B beamline of the National Synchrotron Light Source to allow simultaneous multiwavelength and time-resolved fluorescence detection, as well as spatial imaging of the sample fluorescence. The spectrometer employs monochromatized, tunable UV and visible excitation light from a synchrotron bending magnet and an imaging spectrograph equipped with a single-photon sensitive emission detector. The detector is comprised of microchannel plates in series, with a resistive anode for encoding the position of the photon-derived current. The centroid position of the photon-induced electron cascade is derived in a position analyzer from the four signals measured at the corners of the resistive anode. Spectral information is obtained by dispersing the fluorescence spectrum across one dimension of the detector photocathode. Timing information is obtained by monitoring the voltage divider circuit at the last MCP detector. The signal from the MCP is used as a ''start'' signal to perform a time-correlated single photon counting experiment. The analog signal representing the position, and hence wavelength, is digitized concomitantly with the start/stop time difference and stored in the two-dimensional histogramming memory of a multiparameter analyzer

  11. Improved cryogenic shaft seals

    Science.gov (United States)

    Gillon, W. A., Jr.; Tellier, G. F.

    1976-01-01

    Seals are designed for use with liquid propellant ball valves at temperatures ranging from -400 F to 130 F and 8,000 psig. Seals are capable of sustaining 90 degree rotation, with substantial amount of lateral and axial play, caused by large pressure loads and differential thermal contraction.

  12. Sealed radioactive sources toolkit

    International Nuclear Information System (INIS)

    Mac Kenzie, C.

    2005-09-01

    The IAEA has developed a Sealed Radioactive Sources Toolkit to provide information to key groups about the safety and security of sealed radioactive sources. The key groups addressed are officials in government agencies, medical users, industrial users and the scrap metal industry. The general public may also benefit from an understanding of the fundamentals of radiation safety

  13. Anti-Adhesion Elastomer Seal Coatings for Ultraviolet and Atomic Oxygen Protection

    Science.gov (United States)

    De Groh, Henry C., III; Puleo, Bernadette J.; Waters, Deborah L.; Miller, Sharon K.

    2015-01-01

    Radiation blocking sunscreen coatings have been developed for the protection of elastomer seals used in low-Earth-orbit (LEO). The coatings protect the seals from ultraviolet (UV) radiation and atomic oxygen (AO) damage. The coatings were developed for use on NASA docking seals. Docking seal damage from the UV and AO present in LEO can constrain mission time-line, flight mode options, and increases risk. A low level of adhesion is also required for docking seals so undocking push-off forces can be low. The coatings presented also mitigate this unwanted adhesion. Greases with low collected volatile condensable materials (CVCM) and low total mass loss (TML) were mixed with slippery and/or UV blocking powders to create the protective coatings. Coatings were applied at rates up to 2 milligrams per square centimeter. Coated seals were exposed to AO and UV in the NUV (near-UV) and UV-C wavelength ranges (300 to 400 nanometers and 254 nanometers, respectively). Ground based ashers were used to simulate the AO of space. The Sun's UV energy was mimicked assuming a nose forward flight mode, resulting in an exposure rate of 2.5 megajoules per square meter per day. Exposures between 0 and 147 megajoules per square meter (UV-C) and 245 megajoules per square meter (NUV) were accomplished. The protective coatings were durable, providing protection from UV after a simulated docking and undocking cycle. The level of protection begins to decline at coverage rates less than 0.9 milligrams per square centimeter. The leakage of seals coated with Braycote plus 20 percent Z-cote ZnO sunscreen increased by a factor of 40 after moderate AO exposure; indicating that this coating might not be suitable due to AO intolerance. Seals coated with DC-7-16.4 percent Z-cote ZnO sunscreen were not significantly affected by combined doses of 2 x 10 (sup 21) atoms per square AO with 73 megajoules per square meter UV-C. Unprotected seals were significantly damaged at UV-C exposures of 0.3 megajoules per

  14. Rotary shaft seal

    International Nuclear Information System (INIS)

    Langebrake, C.O.

    1984-01-01

    The invention is a novel rotary shaft seal assembly which provides positive-contact sealing when the shaft is not rotated and which operates with its sealing surfaces separated by a film of compressed ambient gas whose width is independent of the speed of shaft rotation. In a preferred embodiment, the assembly includes a disc affixed to the shaft for rotation therewith. Axially movable, non-rotatable plates respectively supported by sealing bellows are positioned on either side of the disc to be in sealing engagement therewith. Each plate carries piezoelectric transducer elements which are electrically energized at startup to produce films of compressed ambient gas between the confronting surfaces of the plates and the disc. Following shutdown of the shaft, the transducer elements are de-energized. A control circuit responds to incipient rubbing between the plate and either disc by altering the electrical input to the transducer elements to eliminate rubbing

  15. Sealing a conduit end

    International Nuclear Information System (INIS)

    Mentz, R.M.

    1993-01-01

    An apparatus for sealing or blocking conduits, such as the primary nozzles of a nuclear steam generator is described. It includes an annular bracket sealingly attached to the open end of the nozzle, the bracket having a plurality of threaded holes therein. Mounted atop the bracket is a generally circular nozzle dam for covering the opening. Interposed between the nozzle dam and the bracket is an extrusion-resistant seal member having a plurality of apertures therethrough for receiving each bolt. The seal member is configured to resist extrusion by having laminated layers of differing hardnesses, so that the seal member will not laterally extrude away from each bolt in a manner that enlarges the aperture surrounding each bolt as the nozzle dam is bolted to the bracket. (author)

  16. Reactor vessel sealing plug

    International Nuclear Information System (INIS)

    Dooley, R.A.

    1986-01-01

    An apparatus is described for sealing a cold leg nozzle of a nuclear reactor pressure vessel from a remote location comprising: at least one sealing plug for mechanically sealing the nozzle from the inside of the reactor pressure vessel. The sealing plug includes a plate and a cone assembly having an end part receptive in the nozzle, the plate being axially moveable relative to the cone assembly. The plate and cone assembly have confronting bevelled edges defining an opening therebetween. A primary O-ring is disposed about the opening and is supported on the bevelled edges, the plate being guidably mounted to the cone assembly for movement toward the cone assembly to radially expand the primary O-ring into sealing engagement with the nozzle. A means is included for providing relative movement between the outer plate and the cone assembly

  17. Brush seal performance measurement system

    OpenAIRE

    Aksoy, Serdar; Akşit, Mahmut Faruk; Aksit, Mahmut Faruk; Duran, Ertuğrul Tolga; Duran, Ertugrul Tolga

    2009-01-01

    Brush seals are rapidly replacing conventional labyrinth seals in turbomachinery applications. Upon pressure application, seal stiffness increases drastically due to frictional bristle interlocking. Operating stiffness is critical to determine seal wear life. Typically, seal stiffness is measured by pressing a curved shoe to brush bore. The static-unpressurized measurement is extrapolated to pressurized and high speed operating conditions. This work presents a seal stiffness measurement syste...

  18. In vitro study of cytotoxicity by U.V. radiation and differential sensitivity in combination with alkylating agents on established cell systems

    International Nuclear Information System (INIS)

    Ramudu, K.

    1991-01-01

    The effect of U.V. radiation or alkylating agents, such as actinomycin-D, cycloheximide and mitomycin-C (MMC), was studied on CHO, BHK and HeLa cells. U.V. radiation caused DNA ssb and dsb and were prevented by cycloheximide and actinomycin-D. MMC is known to be cytotoxic in CHO/BHK cells by forming free radical generation. MMC in combination with U.V. radiation enhanced DNA ssb ampersand dsb in these cell types. However, HeLa cells were insensitive to U.V. radiation. This insensitivity to U.V. radiation could be ascribed to the presence of glutathione transferase which is absent in CHO/BHK cell line

  19. Reactor cavity seal ring

    International Nuclear Information System (INIS)

    Hankinson, M.F.

    1986-01-01

    A hydrostatic seal is described for sealing an annular gap between two flat substantially horizontal coplanar surfaces comprising, in combination: a generally flat annular plate of a width sufficient to span a gap between two surfaces: compressible annular sealing means disposed on the bottom surface of the flat annular plate for sealingly engaging the two flat surfaces in response to a downward force exerted on the plate; and fastening means, distributed along the center line of the plate, for releasably fastening the plate in a position to span the gap to be sealed and exert a downward force on the plate, each fastening means including a pair of elongated members of a size to fit into the gap to be sealed, means for mounting the members on the bottom surface of the plate so that at least a portion of each member is radially moveable in a direction toward a respective one of the vertical side surfaces defining the gap to be sealed to engage same and so that the plate is moveable relative to the members in a downward direction in response to hydrostatic pressure applied to the upper surface of the plate when the members are engaging the vertical side surfaces of an annular gap, and an actuating means, mounted on the plate for movement therewith in response to hydrostatic pressure, for radially moving the members, the actuating means extending through a bore in the plate to the upper surface of the plate

  20. SEALING SIMULATED LEAKS

    Energy Technology Data Exchange (ETDEWEB)

    Michael A. Romano

    2004-09-01

    This report details the testing equipment, procedures and results performed under Task 7.2 Sealing Simulated Leaks. In terms of our ability to seal leaks identified in the technical topical report, Analysis of Current Field Data, we were 100% successful. In regards to maintaining seal integrity after pigging operations we achieved varying degrees of success. Internal Corrosion defects proved to be the most resistant to the effects of pigging while External Corrosion proved to be the least resistant. Overall, with limitations, pressure activated sealant technology would be a viable option under the right circumstances.

  1. Characterization of curing behavior of UV-curable LSR for LED embedded injection mold

    Science.gov (United States)

    Tae, Joon-Sung; Yim, Kyung-Gyu; Rhee, Byung-Ohk; Kwak, Jae B.

    2016-11-01

    For many applications, liquid silicone rubber (LSR) injection molding is widely used for their great design flexibility and high productivity. In particular, a sealing part for a mobile device such as smartphone and watch has been produced by injection molding. While thermally curable LSR causes deformation problem due to a high mold temperature, UV-curable LSR can be molded at room temperature, which has advantages for over-molding with inserts of temperature-sensitive materials. Ultraviolet light-emitting diodes (UV LEDs) have advantages such as a longer service life, a lower heat dissipation, and smaller size to equip into the mold than conventional halogen or mercury UV lamps. In this work, rheological behavior of UV-curable LSR during curing process was analyzed by UV LEDs available in the market. UV-LEDs of various wave lengths and intensities were tested. The steady shear test was applied to find the starting time of curing and the SAOS was applied to find the ending time of curing to estimate processing time. In addition, the hardness change with irradiation energy was compared with the rheological data to confirm the reliability of the rheological test.

  2. Cover-gas seals: 11-LMFBR seal-test program

    International Nuclear Information System (INIS)

    Steele, O.P. III; Horton, P.H.

    1977-01-01

    The objective of the Cover Gas Seal Material Development Program is to perform the engineering development required to provide reliable seals for LMFBR application. Specific objectives are to verify the performance of commercial solid cross-section and inflatable seals under reactor environments including radiation, to develop advanced materials and configurations capable of achieving significant improvement in radioactive gas containment and seal temperature capabilities, and to optimize seal geometry for maximum reliability and minimal gas permeation

  3. Rotatable seal assembly

    International Nuclear Information System (INIS)

    Garibaldi, J.L.; Logan, C.M.

    1982-01-01

    An assembly is provided for rotatably supporting a rotor on a stator so that vacuum chambers in the rotor and stator remain in communication while the chambers are sealed from ambient air, which enables the use of a ball bearing or the like to support most of the weight of the rotor. The apparatus includes a seal device mounted on the rotor to rotate therewith, but shiftable in position on the rotor while being sealed to the rotor as by an oring. The seal device has a flat face that is biased towards a flat face on the stator, and pressurized air is pumped between the faces to prevent contact between them while spacing them a small distance apart to avoid the inflow of large amounts of air between the faces and into the vacuum chambers

  4. UV Radiation and the Skin

    Directory of Open Access Journals (Sweden)

    Timothy Scott

    2013-06-01

    Full Text Available UV radiation (UV is classified as a “complete carcinogen” because it is both a mutagen and a non-specific damaging agent and has properties of both a tumor initiator and a tumor promoter. In environmental abundance, UV is the most important modifiable risk factor for skin cancer and many other environmentally-influenced skin disorders. However, UV also benefits human health by mediating natural synthesis of vitamin D and endorphins in the skin, therefore UV has complex and mixed effects on human health. Nonetheless, excessive exposure to UV carries profound health risks, including atrophy, pigmentary changes, wrinkling and malignancy. UV is epidemiologically and molecularly linked to the three most common types of skin cancer, basal cell carcinoma, squamous cell carcinoma and malignant melanoma, which together affect more than a million Americans annually. Genetic factors also influence risk of UV-mediated skin disease. Polymorphisms of the melanocortin 1 receptor (MC1R gene, in particular, correlate with fairness of skin, UV sensitivity, and enhanced cancer risk. We are interested in developing UV-protective approaches based on a detailed understanding of molecular events that occur after UV exposure, focusing particularly on epidermal melanization and the role of the MC1R in genome maintenance.

  5. Core disruptive accident margin seal

    International Nuclear Information System (INIS)

    Golden, M.P.

    1979-01-01

    An apparatus for sealing the annulus defined within a substantially cylindrical rotatable riser assembly and plug combination of a nuclear reactor closure head is described. The apparatus comprises an inflatable sealing mechanism disposed in one portion of the riser assembly near the annulus such that upon inflation the sealing mechanism is radially actuated against the other portion of the riser assembly thereby sealing the annulus. The apparatus further comprises a connecting mechanism which places one end of the sealing mechanism in fluid communication with the reactor cover gas so that overpressurization of the reactor cover gas will increase the radial actuation of the sealing mechanism thus enhancing sealing of the annulus

  6. Self-acting shaft seals

    Science.gov (United States)

    Ludwig, L. P.

    1978-01-01

    Self-acting seals are described in detail. The mathematical models for obtaining a seal force balance and the equilibrium operating film thickness are outlined. Particular attention is given to primary ring response (seal vibration) to rotating seat face runout. This response analysis reveals three different vibration models with secondary seal friction being an important parameter. Leakage flow inlet pressure drop and affects of axisymmetric sealing face deformations are discussed. Experimental data on self-acting face seals operating under simulated gas turbine conditions are given. Also a spiral groove seal design operated to 244 m/sec (800 ft/sec) is described.

  7. Sealing arrangement for radioactive material

    International Nuclear Information System (INIS)

    Gray, I.L.S.; Sievwright, R.W.T.; Elliott, J.C.

    1993-01-01

    A sealing arrangement for hermetically sealing two mating surfaces comprises two seals arranged to lie between the surfaces. Each seal provides hermetic sealing over a respective different temperature range and lie serially along the surfaces between the regions to be isolated. A main seal integrity test arrangement is provided in the form of a port and passage. This allows for the introduction of a fluid into or the evacuation of a region between the two seals to detect a leak. The port is also provided with at least two test port seals which seal with a plug. The plug is also provided with a test port to allow the integrity of the test port seal to be tested. (UK)

  8. Nuclear instrumentation cable end seal

    International Nuclear Information System (INIS)

    Cannon, C.P.; Brown, D.P.

    1979-01-01

    An improved coaxial end seal for hermetically sealed nuclear instrumentation cable exhibiting an improved breakdown pulse noise characteristic under high voltage, high temperature conditions is described. A tubular insulator body has metallized interior and exterior surface portions which are braze sealed to a center conductor and an outer conductive sheath. The end surface of the insulator body which is directed toward the coaxial cable to which it is sealed has a recessed surface portion within which the braze seal material terminates

  9. UV/EB curable psa's

    International Nuclear Information System (INIS)

    Glotfelter, C.A.

    1995-01-01

    The author describe both water-based and 100% solids UV/EB curable PSA's (Pressure Sensitive Adhesives) and their properties. A new acrylate monomer, ethoxylated nonyl phenol acrylate, has great utility in the formulation of water-based PSA's

  10. Pressure Actuated Leaf Seals for Improved Turbine Shaft Sealing

    Science.gov (United States)

    Grondahl, Clayton

    2006-01-01

    This presentation introduces a shaft seal in which leaf seal elements are constructed from slotted shim material formed and layered into a frusto-conical assembly. Limited elastic deflection of seal leaves with increasing system pressure close large startup clearance to a small, non-contacting, steady state running clearance. At shutdown seal elements resiliently retract as differential seal pressure diminishes. Large seal clearance during startup and shutdown provides a mechanism for rub avoidance. Minimum operating clearance improves performance and non-contacting operation promises long seal life. Design features of this seal, sample calculations at differential pressures up to 2400 psid and benefit comparison with brush and labyrinth seals is documented in paper, AIAA 2005 3985, presented at the Advanced Seal Technology session of the Joint Propulsion Conference in Tucson this past July. In this presentation use of bimetallic leaf material will be discussed. Frictional heating of bimetallic leaf seals during a seal rub can relieve the rub condition to some extent with a change in seal shape. Improved leaf seal rub tolerance is expected with bimetallic material.

  11. Upgrading inflatable door seals

    International Nuclear Information System (INIS)

    Sykes, T.M.; Metcalfe, R.; Welch, L.A.; Josefowich, J.M.

    1997-01-01

    Inflatable door seals are used for airlocks in CANDU stations. They have been a significant source of unreliability and maintenance cost. A program is underway to improve their performance and reliability, backed by environmental qualification testing. Only commercial products and suppliers existed in 1993. For historical reasons, these 'existing products' did not use the most durable material then available. In hindsight, neither had they been adapted nor optimized to combat conditions often experienced in the plants-sagging doors, damaged sealing surfaces, and many thousands of openings and closings per year. Initial attempts to involve the two existing suppliers in efforts to upgrade these seals were unsuccessful. Another suitable supplier had therefore to be found, and a 'new,' COG-owned seal developed; this was completed in 1997. This paper summarizes its testing, along with that of the two existing products. Resistance to aging has been improved significantly. Testing has shown that an accident can be safely withstood after 10 years of service or 40,000 openings-closings, whichever comes first. AECL's Fluid Sealing Technology Unit (FSTU) has invested in the special moulds, test fixtures and other necessary tooling and documentation required to begin commercial manufacture of this new quality product. Accordingly, as with FSTU's other nuclear products such as pump seals, the long-term supply of door seals to CANDU plants is now protected from many external uncertainties-e.g., commercial products being discontinued, materials being changed, companies going out of business. Manufacturing to AECL's detailed specifications is being subcontracted to the new supplier. FSTU is performing the quality surveillance, inspection, testing, and customer service activities concomitant with direct responsibility for supply to the plants. (author)

  12. Materials for water pump mechanical seals

    International Nuclear Information System (INIS)

    Brousse, P.

    1992-01-01

    In view of the continually increasing power ratings of conventional and nuclear power plants and the related reliability and safety problems, plant builders have had to develop seal systems compatible with current water pump performances. In 1970, EDF/R and DD was already concerned by this problem. It soon became obvious that the nature of the materials used for the friction surfaces was decisive for seal durability. Exceptional loads (transients, high vibration levels, etc...) hasten aging. To begin with, friction surfaces consisted of a hard material (tungsten carbide) mated with a soft material (carbon). Resistance was unpredictable and not compatible with industrial requirements. Tests performed on the EDF/R and DD test benches evidenced the same types of degradation. The mechanical seal manufacturers then began to use ceramic materials (silicon carbide), which raised high expectations. Unfortunately, these were recent materials and their manufacturing process was not thoroughly understood. Hopes were soon dashed in many applications, including that of mechanical seals. Fluctuating results were obtained over the next few years. The raw material suppliers made progress, especially with regard to reducing fragility. On a parallel, the mechanical seal manufacturers initiated comparative tests on the friction resistance of materials. It has also been established that ceramics have to be stringently supervised at all levels: part design, inspection, assembly, use. EDF has much insisted that mechanical seal suppliers guarantee the constant quality of their products. EDF/R and DD has systematically tested new sensitive devices, under normal and exceptional conditions, prior to their installation at the plants. At the present time, the silicon carbides proposed by the mechanical seal suppliers are entirely satisfactory. The carbon mating surface was far less problematic. The required reliability was obtained by replacing resin binder carbons by the more resistant

  13. Shaft seal assembly and method

    Science.gov (United States)

    Keba, John E. (Inventor)

    2007-01-01

    A pressure-actuated shaft seal assembly and associated method for controlling the flow of fluid adjacent a rotatable shaft are provided. The seal assembly includes one or more seal members that can be adjusted between open and closed positions, for example, according to the rotational speed of the shaft. For example, the seal member can be configured to be adjusted according to a radial pressure differential in a fluid that varies with the rotational speed of the shaft. In addition, in the closed position, each seal member can contact a rotatable member connected to the shaft to form a seal with the rotatable member and prevent fluid from flowing through the assembly. Thus, the seal can be closed at low speeds of operation and opened at high speeds of operation, thereby reducing the heat and wear in the seal assembly while maintaining a sufficient seal during all speeds of operation.

  14. High sensitivity but normal DNA-repair activity after UV irradiation in Epstein-Barr virus-transformed lymphoblastoid cell lines from Chediak-Higashi syndrome

    International Nuclear Information System (INIS)

    Tanaka, H.; Orii, T.

    1980-01-01

    We established lymphoblastoid cell lines from 2 children with Chediak-Higashi syndrome (CHS), 2 xeroderma pigmentosum (XP) patients and control donors after transformation of peripheral lymphocytes by Epstein-Barr virus (EBV). We used these lymphoblastoid cell lines to investigate repair activity after ultraviolet irradiation. Cell survival of both CHS lymphoblastoid cell lines after irradiation by UV and treatment by 4-nitroquinoline 1-oxide (4NQO) fell between those of the XP and control cells lines. Unscheduled DNA synthesis of CHS cells after UV irradiation occured at rates similar to those of control cells. (orig.)

  15. UV Tanning Equipment | Radiation Protection | US EPA

    Science.gov (United States)

    2017-08-07

    Sun lamps and tanning equipment emit ultraviolet (UV) rays. People who are exposed to UV rays over a long period of time are more likely to develop skin cancer. People with light skin are in more danger because their skin is more sensitive to UV rays.

  16. Pool gateway seal

    International Nuclear Information System (INIS)

    Starr, J.A.; Steinert, L.A.

    1983-01-01

    A device for sealing a gateway between interconnectable pools in a nuclear facility comprising a frame supporting a liquid impermeable sheet positioned in a u-shaped gateway between the pools. An inflatable tube carried in a channel in the periphery of the frame and adjoining the gateway provides a seal therebetween when inflated. A restraining arrangement on the bottom edge of the frame is releasably engagable with an adjacent portion of the gateway to restrict the movement of the frame in the u-shaped gateway upon inflation of the tube, thereby enhancing the seal. The impermeable sheet is formed of an elastomer and thus is conformable to a liquid permeable supportive wall upon application of liquid pressure to the side of the sheet opposite the wall

  17. Action of cis-dichlorobis (cyclopentylamine) platinum (2) (cis-PAD) on L5178Y cells of two strains inversely cross-sensitive to X-rays and UV-light. Part 1. Cytotoxicity

    International Nuclear Information System (INIS)

    Szumiel, I.

    1977-01-01

    The response to cis-PAD, an antitumour platinum complex, was studied in two strains of murine lymphoma L5178Y cross-sensitive to X-rays and UV light. Dose-survival relationship, DNA synthesis, formation of chromatid aberrations, progression through the cell cycle, and growth and viability changes after 1 h cis-PAD treatment at 37 0 C were examined and compared with respective effects of X-rays and UV-light. In both strains studied, cis-PAD causes immediate inhibition of progression through the cell cycle, reduced rate of DNA synthesis, delayed appearance of chromatid aberrations and delayed death. However, there is a marked difference in sensitivity to cis-PAD between L5178Y-S strain (D 0 ca.5.8 μg/ml) and L5178Y-R strain (D 0 ca. 2.5μg/ml). In both strains a close resemblance was found between dose-survival relationship after cis-PAD and UV-light treatment, respectively. (author)

  18. HMSRP Hawaiian Monk Seal Master Identification Records (seal)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains records of all individually identified Hawaiian monk seals since 1981. These seals were identified by PSD personnel and cooperating scientists...

  19. Acute exposure to UV-B sensitizes cucumber, tomato, and Arabidopsis plants to photooxidative stress by inhibiting thermal energy dissipation and antioxidant defense

    International Nuclear Information System (INIS)

    Moon, Yu-Ran; Lee, Min-Hee; Chung, Byung-Yeoup; Kim, Jin-Hong; Tovuu, Altanzaya; Lee, Choon-Hwan; Park, Youn-Il

    2011-01-01

    The purpose of this study was to characterize a change in Non-photochemical quenching (NPQ) upon exposure to ultraviolet-B (UV-B), the xanthophyll cycle-dependent and -independent NPQs were compared in Cucumis sativus, Lycopersicum esculentum, and Arabidopsis thaliana leaves. The xanthophyll cycle-dependent NPQ was dramatically but reversibly suppressed by UV-B radiation. This suppression was correlated more strongly with a marked decrease in photosynthetic electron transport rather than changes in xanthophyll cycle enzymes such as violaxanthin de-epoxidase and zeaxanthin epoxidase. Accordingly, the UV-B-induced suppression of NPQ cannot be attributed to changes in expressions of violaxanthin de-epoxidase (VDE) and zeaxanthin epoxidase (ZEP). However, suppression of the xanthophyll cycle-dependent NPQ could only account for the 77 K fluorescence emission spectra of thylakoid membranes and the increased level of 1 O 2 production, but not for the decreased levels of hydroxyl radical O 2 - production and H 2 O 2 scavenging. These results suggest that a gradual reduction of H 2 O 2 scavenging activity as well as a transient and reversible suppression of thermal energy dissipation may contribute differentially to increased photooxidative damages in cucumber, tomato, and Arabidopsis plants after acute exposure to UV-B radiation. (author)

  20. Dye filled security seal

    International Nuclear Information System (INIS)

    Wilson, D.C.

    1982-01-01

    A security seal for providing an indication of unauthorized access to a sealed object includes an elongate member to be entwined in the object such that access is denied unless the member is removed. The elongate member has a hollow, pressurizable chamber extending throughout its length that is filled with a permanent dye under greater than atmospheric pressure. Attempts to cut the member and weld it together are revealed when dye flows through a rupture in the chamber wall and stains the outside surface of the member

  1. Enhancement of UV photodetector properties of ZnO nanorods/PEDOT:PSS Schottky junction by NGQD sensitization along with conductivity improvement of PEDOT:PSS by DMSO additive

    Science.gov (United States)

    Dhar, Saurab; Majumder, Tanmoy; Chakraborty, Pinak; Mondal, Suvra Prakash

    2018-04-01

    Schottky junction ultraviolet (UV) photodetector was fabricated by spin coating a hole conducting polymer, poly 3,4-ethylenedioxythiophene: polystyrene sulfonate (PEDOT:PSS) on hydrothermally grown zinc oxide (ZnO) nanorod arrays. The UV detector performance was significantly improved two step process. Firstly, ZnO nanorods were modified by sensitizing N doped grapheme quantum dots (NGQDs) for better photoresponce behavior. Afterwards, the junction properties as well as photoresponse was enhanced by modifying electrical conductivity of PEDOT:PSS layer with organic solvent (DMSO). Our NGQD decorated ZnO NRs/DMSO-PEDOT:PSS Schottky junction device demonstrated superior external quantum efficiency (EQE ˜ 90063 %) and responsivity (Rλ˜247 A/W) at 340 nm wavelength and -1V external bias. The response and recovery times of the final photodetector device was very fast compared to GQD as well as NGQD modified and pristine ZnO nanorod based detectors.

  2. Cost saving synergistic shaft seal

    Science.gov (United States)

    Ludwig, L. P.; Strom, T. N.

    1976-01-01

    Segmented carbon rings, used to replace elastomeric seal lip, provide resistance to high temperatures generated in lubricating film. Machining and close manufacturing tolerances of conventional segmented seal are avoided by mounting segmented rings in elastomeric flex section.

  3. Magnetically Actuated Seal, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — FTT proposes development of a magnetically actuated dynamic seal. Dynamic seals are used throughout the turbopump in high-performance, pump-fed, liquid rocket...

  4. Magnetically Actuated Seal, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — FTT proposes development of a magnetically actuated dynamic seal. Dynamic seals are used throughout the turbopump in high-performance, pump-fed, liquid rocket...

  5. Continuous improvement of pump seals

    International Nuclear Information System (INIS)

    Wong, W.; Eyvindson, A.; Rhodes, D.B.

    2003-01-01

    Pump seal reliability continues to be an area needing improvement and ongoing vigilance. Methods have been developed for identifying and assessing factors relating to seal performance, selecting the most relevant ones for a specific station, and then focusing on the most significant aspects and how to improve. Discussion invariably addresses maintenance practices, seal design, monitoring capabilities, operating conditions, transients, and pump and motor design. Success in reliability improvement requires ongoing dialogue among the station operators, pump manufacturers and seal designers. AECL CAN-seals lead the nuclear industry in reliability and seal life. They effectively save operators millions of dollars in outage time and person-rem. This paper describes some of the significant developments in AECL's ongoing program in seal R and D, as well as recent new installations following the most demanding seal qualification programs to date. (author)

  6. Intratracheal Seal Disc

    DEFF Research Database (Denmark)

    Christiansen, Karen J; Moeslund, Niels; Lauridsen, Henrik

    2017-01-01

    . The device consisted of an intratracheal silicone seal disc fixated by a cord through the stoma to an external part. At day 14, computed tomography (CT) was performed before the device was extracted. With the pulling of a cord, the disc unraveled into a thin thread and was extracted through the stoma. At day...

  7. GOLD PRESSURE VESSEL SEAL

    Science.gov (United States)

    Smith, A.E.

    1963-11-26

    An improved seal between the piston and die member of a piston-cylinder type pressure vessel is presented. A layer of gold, of sufficient thickness to provide an interference fit between the piston and die member, is plated on the contacting surface of at least one of the members. (AEC)

  8. Shaft Seal Compensates for Cold Flow

    Science.gov (United States)

    Myers, W. N.; Hein, L. A.

    1985-01-01

    Seal components easy to install. Ring seal for rotating or reciprocating shafts spring-loaded to compensate for slow yielding (cold flow) of sealing material. New seal relatively easy to install because components preassembled, then installed in one piece.

  9. Built-in potential shift and Schottky-barrier narrowing in organic solar cells with UV-sensitive electron transport layers.

    Science.gov (United States)

    Li, Cheng; Credgington, Dan; Ko, Doo-Hyun; Rong, Zhuxia; Wang, Jianpu; Greenham, Neil C

    2014-06-28

    The performance of organic solar cells incorporating solution-processed titanium suboxide (TiOx) as electron-collecting layers can be improved by UV illumination. We study the mechanism of this improvement using electrical measurements and electroabsorption spectroscopy. We propose a model in which UV illumination modifies the effective work function of the oxide layer through a significant increase in its free electron density. This leads to a dramatic improvement in device power conversion efficiency through several mechanisms - increasing the built-in potential by 0.3 V, increasing the conductivity of the TiOx layer and narrowing the interfacial Schottky barrier between the suboxide and the underlying transparent electrode. This work highlights the importance of considering Fermi-level equilibration when designing multi-layer transparent electrodes.

  10. Fiber Optic Safeguards Sealing System

    Science.gov (United States)

    1978-01-01

    8217 or trade names does not constitute an official indorsement or approval of the use thereof. Destroy this report when it is no longer needed. Do not...an intergrity check of a seal than to photograph the seal’s fingerprints and to match positive/negative overlays. The seal identification time and

  11. Ultrasonic dip seal maintenance system

    International Nuclear Information System (INIS)

    Poindexter, A.M.; Ricks, H.E.

    1978-01-01

    Disclosed is a system for removing impurities from the surfaces of liquid dip seals and for wetting the metal surfaces of liquid dip seals in nuclear components. The system comprises an ultrasonic transducer that transmits ultrasonic vibrations along an ultrasonic probe to the metal and liquid surfaces of the dip seal thereby loosening and removing those impurities

  12. Radial lip seals, thermal aspects

    NARCIS (Netherlands)

    Stakenborg, M.J.L.; van Ostaijen, R.A.J.; Dowson, D.

    1989-01-01

    In this paper the influence of temperature on tne seal-snarc contact is studied, using coupled temperature-stress FEH analysis. A thermal network model is used to calculate the seal-shaft contact temperature for steady-state and transient conditions. Contact temperatures were measured under the seal

  13. Cover gas seals: FFTF-LMFBR seal test program

    International Nuclear Information System (INIS)

    Kurzeka, W.; Oliva, R.; Welch, T.S.; Shimazaki, T.

    1974-01-01

    The objectives of this program are to: (1) conduct static and dynamic tests to demonstrate or determine the mechanical performance of full-size (cross section) FFTF fuel transfer machine and reactor vessel head seals intended for use in a sodium vapor-inert gas environment, (2) demonstrate that these FFTF seals or new seal configurations provide acceptable fission product and cover gas retention capabilities at Clinch River Breeder Reactor Plant (CRBRP) operating environmental conditions other than radiation, and (3) develop improved seals and seal technology for the CRBRP to support the national objective to reduce all atmospheric contaminations to low levels

  14. Failure analysis and seal life prediction for contacting mechanical seals

    Science.gov (United States)

    Sun, J. J.; He, X. Y.; Wei, L.; Feng, X.

    2008-11-01

    Fault tree analysis method was applied to quantitatively investigate the causes of the leakage failure of mechanical seals. It is pointed out that the change of the surface topography is the main reasons causing the leakage of mechanical seals under the condition of constant preloads. Based on the fractal geometry theory, the relationship between the surface topography and working time were investigated by experiments, and the effects of unit load acting on seal face on leakage path in a mechanical seal were analyzed. The model of predicting seal life of mechanical seals was established on the basis of the relationship between the surface topography and working time and allowable leakage. The seal life of 108 mechanical seal operating at the system of diesel fuel storage and transportation was predicted and the problem of the condition monitoring for the long-period operation of mechanical seal was discussed by this method. The research results indicate that the method of predicting seal life of mechanical seals is feasible, and also is foundation to make scheduled maintenance time and to achieve safe-reliability and low-cost operation for industrial devices.

  15. Effect of uvs1, uvs2 and xrs mutations on the radiosensitivity and the induced mitotic recombination frequency in diploid yeast cells

    International Nuclear Information System (INIS)

    Suslova, N.G.; Fedorova, I.V.; Zheleznyakova, N.Yu.

    1975-01-01

    The influence of the loci of radiosensitivity uvs1, uvs2, and xrs in the homozygous state at the diploid level on the sensitivity to UV and ionizing radiation and induced mitotic recombination was studied in the yeast Sacch. cerevisiae. Hypersensitivity to UV irradiation was detected in the diploids uvs2 uvs2 xrs xrs in comparision with the corresponding control. The diploid uvs1 uvs1 uvs2 uvs2 does not differ in UV sensitivity from the diploid uvs1 uvs1 UVS2 UVS2. These facts demonstrate that the uvs1 and uvs2 mutations, on the one hand, and the xrs mutations, on the other, normally control different pathways of elimination of UV-induced damages. It was shown that the diploid uvs2 uvs2 xrs3 xrs3 is far more sensitive to the lethal action of x rays than the control diploid UVS2 UVS2 xrs3 xrs3. Consequently, the mutations uvs2 and xrs3 block different modes of repair of damages induced by ionizing radiation. In all the double-mutant diploids, the frequency of mitotic recombination induced by UV rays increases sharply in comparison with that of the radioresistant diploids UVS UVS XRS XRS and the UV-sensitive diploids uvs2 uvs2 XRS XRS. Possible causes of the observed phenomenon are discussed. It was established that in a diploid homozygous for the loci uvs2 xrs5, the frequency of mitotic recombination induced by x rays increases extremely sharply. This fact confirms the hypothesis that the gene product of the locus uvs2 participates in the repair of DNA after the action of ionizing radiation. (author)

  16. Refrigeration system with clearance seals

    International Nuclear Information System (INIS)

    Holland, N. J.

    1985-01-01

    In a refrigeration system such as a split Stirling system, fluid seals associated with the reciprocating displacer are virtually dragless clearance seals. Movement of the displacer relative to the pressure variations in the working volume of gas is retarded by a discrete braking element. Because it is not necessary that the brake providing any sealing action, the brake can be designed for greater durability and less dependence on ambient and operating temperatures. Similarly, the clearance seal can be formed of elements having low thermal expansion such that the seal is not temperature dependent. In the primary embodiments the braking element is a split friction brake

  17. Hermetic Seal Leak Detection Apparatus

    Science.gov (United States)

    Kelley, Anthony R. (Inventor)

    2013-01-01

    The present invention is a hermetic seal leak detection apparatus, which can be used to test for hermetic seal leaks in instruments and containers. A vacuum tight chamber is created around the unit being tested to minimize gas space outside of the hermetic seal. A vacuum inducing device is then used to increase the gas chamber volume inside the device, so that a slight vacuum is pulled on the unit being tested. The pressure in the unit being tested will stabilize. If the stabilized pressure reads close to a known good seal calibration, there is not a leak in the seal. If the stabilized pressure reads closer to a known bad seal calibration value, there is a leak in the seal. The speed of the plunger can be varied and by evaluating the resulting pressure change rates and final values, the leak rate/size can be accurately calculated.

  18. Regenerator cross arm seal assembly

    Science.gov (United States)

    Jackman, Anthony V.

    1988-01-01

    A seal assembly for disposition between a cross arm on a gas turbine engine block and a regenerator disc, the seal assembly including a platform coextensive with the cross arm, a seal and wear layer sealingly and slidingly engaging the regenerator disc, a porous and compliant support layer between the platform and the seal and wear layer porous enough to permit flow of cooling air therethrough and compliant to accommodate relative thermal growth and distortion, a dike between the seal and wear layer and the platform for preventing cross flow through the support layer between engine exhaust and pressurized air passages, and air diversion passages for directing unregenerated pressurized air through the support layer to cool the seal and wear layer and then back into the flow of regenerated pressurized air.

  19. Sealing wells with gel

    Energy Technology Data Exchange (ETDEWEB)

    Lopez, E C

    1967-10-01

    A new system is being used in Mexico to temporarily plug producing wells. The temporary seal is a gel with a catalyst. The use of this temporary plug allows gas-lift wells to be taken off production in order to carry out emergency repairs. The gel solidifies by the action of the catalyst to a high temperature (70 - 150/sup 0/C). By locating the bottom of the tubing at the top of the production interval, the gel material will go into the permeable formation, and immediately set. When the gel has solidified, it seals off the horizon that must not be stimulated, and leaves the others exposed to the acid action. When the treatment is finished, the gel, by action of the catalyst, is liquefied and removed from the formation, being produced with the oil.

  20. Radioactive waste sealing container

    International Nuclear Information System (INIS)

    Tozawa, S.; Kitamura, T.; Sugimoto, S.

    1984-01-01

    A low- to medium-level radioactive waste sealing container is constructed by depositing a foundation coating consisting essentially of zinc, cadmium or a zinc-aluminum alloy over a steel base, then coating an organic synthetic resin paint containing a metal phosphate over the foundation coating, and thereafter coating an acryl resin, epoxy resin, and/or polyurethane paint. The sealing container can consist of a main container body, a lid placed over the main body, and fixing members for clamping and fixing the lid to the main body. Each fixing member may consist of a material obtained by depositing a coating consisting essentially of cadmium or a zinc-aluminum alloy over a steel base

  1. Electromagnetic shaft seal

    International Nuclear Information System (INIS)

    Takahashi, Kenji.

    1994-01-01

    As an electromagnetic shaft seal, there are disposed outwarding electromagnetic induction devices having generating power directing to an electroconductive fluid as an object of sealing, and inwarding electromagnetic induction device added coaxially. There are disposed elongate rectangular looped first coils having a predetermined inner diameter, second coils having the same shape and shifted by a predetermined pitch relative to the first coil and third coil having the same shape and shifted by a predetermined pitch relative to the second coil respectively each at a predetermined inner diameter of clearance to the outwarding electromagnetic induction devices and the inwarding electromagnetic induction device. If the inwarding electromagnetic induction device and the outwarding electromagnetic induction device are operated, they are stopped at a point that the generating power of the former is equal with the sum of the generating power of the latter and a differential pressure. When three-phase AC is charged to the first coil, the second coil and the third coil successively, a force is generated in the advancing direction of the magnetic field in the electroconductive fluid by the similar effect to that of a linear motor, and the seal is maintained at high reliability. Moreover, the limit for the rotational angle of the shaft is not caused. (N.H.)

  2. Spectroscopic (FT-IR, FT-Raman and UV-Visible) investigations, NMR chemical shielding anisotropy (CSA) parameters of 2,6-Diamino-4-chloropyrimidine for dye sensitized solar cells using density functional theory.

    Science.gov (United States)

    Gladis Anitha, E; Joseph Vedhagiri, S; Parimala, K

    2015-02-05

    The molecular structure, geometry optimization, vibrational frequencies of organic dye sensitizer 2,6-Diamino-4-chloropyrimidine (DACP) were studied based on Hartree-Fock (HF) and density functional theory (DFT) using B3LYP methods with 6-311++G(d,p) basis set. Ultraviolet-Visible (UV-Vis) spectrum was investigated by time dependent DFT (TD-DFT). Features of the electronic absorption spectrum in the UV-Visible regions were assigned based on TD-DFT calculation. The absorption bands are assigned to transitions. The interfacial electron transfer between semiconductor TiO2 electrode and dye sensitizer DACP is due to an electron injection process from excited dye to the semiconductor's conduction band. The observed and the calculated frequencies are found to be in good agreement. The energies of the frontier molecular orbitals (FMOS) have also been determined. The chemical shielding anisotropic (CSA) parameters are calculated from the NMR analysis, Stability of the molecule arising from hyperconjugative interactions and charge delocalization has been analyzed using natural bond orbital (NBO) analysis. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Closure and Sealing Design Calculation

    International Nuclear Information System (INIS)

    T. Lahnalampi; J. Case

    2005-01-01

    The purpose of the ''Closure and Sealing Design Calculation'' is to illustrate closure and sealing methods for sealing shafts, ramps, and identify boreholes that require sealing in order to limit the potential of water infiltration. In addition, this calculation will provide a description of the magma that can reduce the consequences of an igneous event intersecting the repository. This calculation will also include a listing of the project requirements related to closure and sealing. The scope of this calculation is to: summarize applicable project requirements and codes relating to backfilling nonemplacement openings, removal of uncommitted materials from the subsurface, installation of drip shields, and erecting monuments; compile an inventory of boreholes that are found in the area of the subsurface repository; describe the magma bulkhead feature and location; and include figures for the proposed shaft and ramp seals. The objective of this calculation is to: categorize the boreholes for sealing by depth and proximity to the subsurface repository; develop drawing figures which show the location and geometry for the magma bulkhead; include the shaft seal figures and a proposed construction sequence; and include the ramp seal figure and a proposed construction sequence. The intent of this closure and sealing calculation is to support the License Application by providing a description of the closure and sealing methods for the Safety Analysis Report. The closure and sealing calculation will also provide input for Post Closure Activities by describing the location of the magma bulkhead. This calculation is limited to describing the final configuration of the sealing and backfill systems for the underground area. The methods and procedures used to place the backfill and remove uncommitted materials (such as concrete) from the repository and detailed design of the magma bulkhead will be the subject of separate analyses or calculations. Post-closure monitoring will not

  4. TECHNOLOGY ROADMAPPING FOR IAEA SEALS.

    Energy Technology Data Exchange (ETDEWEB)

    HOFFHEINS,B.; ANNESE,C.; GOODMAN,M.; OCONNOR,W.; GUSHUE,S.; PEPPER,S.

    2003-07-13

    In the fall of 2002, the U.S. Support Program (USSP) initiated an effort to define a strategy or ''roadmap'' for future seals technologies and to develop a generalized process for planning safeguards equipment development, which includes seals and other safeguards equipment. The underlying objectives of the USSP include becoming more proactive than reactive in addressing safeguards equipment needs, helping the IAEA to maintain an inventory of cost-effective, reliable, and effective safeguards equipment, establishing a long-term planning horizon, and securing IAEA ownership in the process of effective requirements definition and timely transitioning of new or improved systems for IAEA use. At an initial workshop, seals, their functions, performance issues, and future embodiments were discussed in the following order: adhesive seals, metal seals, passive and active loop seals, ultrasonic seals, tamper indicating enclosures (including sample containers, equipment enclosures, and conduits). Suggested improvements to these technologies focused largely on a few themes: (1) The seals must be applied quickly, easily, and correctly; (2) Seals and their associated equipment should not unduly add bulk or weight to the inspectors load; (3) Rapid, in-situ verifiability of seals is desirable; and (4) Seal systems for high risk or high value applications should have two-way, remote communications. Based upon these observations and other insights, the participants constructed a skeletal approach for seals technology planning. The process begins with a top-level review of the fundamental safeguards requirements and extraction of required system features, which is followed by analysis of suitable technologies and identification of technology gaps, and finally by development of a planning schedule for system improvements and new technology integration. Development of a comprehensive procedure will require the partnership and participation of the IAEA. The

  5. Mitoxantrone Loaded Superparamagnetic Nanoparticles for Drug Targeting: A Versatile and Sensitive Method for Quantification of Drug Enrichment in Rabbit Tissues Using HPLC-UV

    Directory of Open Access Journals (Sweden)

    Rainer Tietze

    2010-01-01

    Full Text Available In medicine, superparamagnetic nanoparticles bound to chemotherapeutics are currently investigated for their feasibility in local tumor therapy. After intraarterial application, these particles can be accumulated in the targeted area by an external magnetic field to increase the drug concentration in the region of interest (Magnetic-Drug-Targeting. We here present an analytical method (HPLC-UV, to detect pure or ferrofluid-bound mitoxantrone in a complex matrix even in trace amounts in order to perform biodistribution studies. Mitoxantrone could be extracted in high yields from different tissues. Recovery of mitoxantrone in liver tissue (5000 ng/g was 76±2%. The limit of quantification of mitoxantrone standard was 10 ng/mL ±12%. Validation criteria such as linearity, precision, and stability were evaluated in ranges achieving the FDA requirements. As shown for pilot samples, biodistribution studies can easily be performed after application of pure or ferrofluid-bound mitoxantrone.

  6. Shaft and tunnel sealing considerations

    International Nuclear Information System (INIS)

    Kelsall, P.C.; Shukla, D.K.

    1980-01-01

    Much of the emphasis of previous repository sealing research has been placed on plugging small diameter boreholes. It is increasingly evident that equal emphasis should now be given to shafts and tunnels which constitute more significant pathways between a repository and the biosphere. The paper discusses differences in requirements for sealing shafts and tunnels as compared with boreholes and the implications for seal design. Consideration is given to a design approach for shaft and tunnel seals based on a multiple component design concept, taking into account the requirements for retrievability of the waste. A work plan is developed for the future studies required to advance shaft and tunnel sealing technology to a level comparable with the existing technology for borehole sealing

  7. Sealed radioactive source management

    International Nuclear Information System (INIS)

    2005-01-01

    Sealed radioactive sources have been used in a wide range of application in medicine, agriculture, geology, industry and other fields. Since its utilization many sources have become out of use and became waste but no proper management. This has lead to many accidents causing deaths and serious radiation injuries worldwide. Spent sources application is expanding but their management has seen little improvements. Sealed radioactive sources have become a security risk calling for prompt action. Source management helps to maintain sources in a good physical status and provide means of source tracking and control. It also provides a well documented process of the sources making any future management options safe, secure and cost effective. Last but not least good source management substantially reduces the risk of accidents and eliminates the risk of malicious use. The International Atomic Energy Agency assists Member States to build the infrastructure to properly manage sealed radioactive sources. The assistance includes training of national experts to handle, condition and properly store the sources. For Member States that do not have proper facilities, we provide the technical assistance to design a proper facility to properly manage the radioactive sources and provide for their proper storage. For Member States that need to condition their sources properly but don't have the required infrastructure we provide direct assistance to physically help them with source recovery and provide an international expert team to properly condition their sources and render them safe and secure. We offer software (Radioactive Waste Management Registry) to properly keep a complete record on the sources and provide for efficient tracking. This also helps with proper planning and decision making for long term management

  8. Sealing of rock fractures

    International Nuclear Information System (INIS)

    Pusch, R.; Erlstroem, M.; Boergesson, L.

    1985-12-01

    The major water-bearing fractures in granite usually from fairly regular sets but the extension and degree of connectivity is varying. This means that only a few fractures that are interconnected with the deposition holes and larger water-bearing structures in a HLW repository are expected and if they can be identified and cut off through sealing it would be possible to improve the isolation of waste packages very effectively. Nature's own fracture sealing mechanisms may be simulated and a survey of the involved processes actually suggests a number of possible filling methods and substances. Most of them require high temperature and pressure and correspondingly sophisticated techniques, but some are of potential interest for immediate application with rather moderate effort. Such a technique is to fill the fractures with clayey substances which stay flexible and low-permeable provided that they remain physically and chemically intact. It is demonstrated in the report that effective grouting requires a very low viscosity and shear strength of the substance and this can be achieved by mechanical agitation as demonstrated in this report. Thus, by superimposing static pressure and shear waves induced by percussion hammering at a suitable frequency, clays and fine-grained silts as well as cement can be driven into fractures with an average aperture as small as 0.1 mm. Experiments were made in the laboratory using concrete and steel plates, and a field pilot test was also conducted under realistic conditions on site in Stripa. They all demonstrated the practicality of the 'dynamic injection technique' and that the fluid condition of the grouts yielded complete filling of the injected space to a considerable distance from the injection point. The field test indicated a good sealing ability as well as a surprisingly high resistance to erosion and piping. (author)

  9. Low-Torque Seal Development

    Science.gov (United States)

    Lattime, Scott B.; Borowski, Richard

    2009-01-01

    The EcoTurn Class K production prototypes have passed all AAR qualification tests and received conditional approval. The accelerated life test on the second set of seals is in progress. Due to the performance of the first set, no problems are expected.The seal has demonstrated superior performance over the HDL seal in the test lab with virtually zero torque and excellent contamination exclusion and grease retention.

  10. Production of sealed sources

    International Nuclear Information System (INIS)

    Bandi, L.N.

    2016-01-01

    Radioisotope production has been an ongoing activity in India since the sixties. Radioisotopes find wide-ranging applications in various fields, including industry, research, agriculture and medicine. Board of Radiation and Isotope Technology, an industrial unit of Department of Atomic Energy is involved in fabrication and supply of wide variety of sealed sources. The main radioisotopes fabricated and supplied by BRIT are Cobalt-60, Iridium-192. These isotopes are employed in industrial and laboratory irradiators, teletherapy machines, radiography exposure devices, nucleonic gauges. The source fabrication facilities of BRIT are located at Rajasthan Atomic Power Project Cobalt-60 Facility (RAPPCOF), Kota, Radiological Laboratories Group (RLG) and High Intensity Radiation Utilization Project (HIRUP) at Trombay

  11. SEAL FOR HIGH SPEED CENTRIFUGE

    Science.gov (United States)

    Skarstrom, C.W.

    1957-12-17

    A seal is described for a high speed centrifuge wherein the centrifugal force of rotation acts on the gasket to form a tight seal. The cylindrical rotating bowl of the centrifuge contains a closure member resting on a shoulder in the bowl wall having a lower surface containing bands of gasket material, parallel and adjacent to the cylinder wall. As the centrifuge speed increases, centrifugal force acts on the bands of gasket material forcing them in to a sealing contact against the cylinder wall. This arrangememt forms a simple and effective seal for high speed centrifuges, replacing more costly methods such as welding a closure in place.

  12. Advanced High Temperature Structural Seals

    Science.gov (United States)

    Newquist, Charles W.; Verzemnieks, Juris; Keller, Peter C.; Rorabaugh, Michael; Shorey, Mark

    2002-10-01

    This program addresses the development of high temperature structural seals for control surfaces for a new generation of small reusable launch vehicles. Successful development will contribute significantly to the mission goal of reducing launch cost for small, 200 to 300 pound payloads. Development of high temperature seals is mission enabling. For instance, ineffective control surface seals can result in high temperature (3100 F) flows in the elevon area exceeding structural material limits. Longer sealing life will allow use for many missions before replacement, contributing to the reduction of hardware, operation and launch costs.

  13. Borehole sealing method and apparatus

    International Nuclear Information System (INIS)

    Hartley, J.N.; Jansen, G. Jr.

    1977-01-01

    A method and apparatus is described for sealing boreholes in the earth. The borehole is blocked at the sealing level, and a sealing apparatus capable of melting rock and earth is positioned in the borehole just above seal level. The apparatus is heated to rock-melting temperature and powdered rock or other sealing material is transported down the borehole to the apparatus where it is melted, pooling on the mechanical block and allowed to cool and solidify, sealing the hole. Any length of the borehole can be sealed by slowly raising the apparatus in the borehole while continuously supplying powdered rock to the apparatus to be melted and added to the top of the column of molten and cooling rock, forming a continuous borehole seal. The sealing apparatus consists of a heater capable of melting rock, including means for supplying power to the heater, means for transporting powdered rock down the borehole to the heater, means for cooling the apparatus and means for positioning the apparatus in the borehole. 5 claims, 1 figure

  14. Thermo-Mechanical Test of Seal System in Flexible Pipe End Fittings

    DEFF Research Database (Denmark)

    Banke, Lars

    1999-01-01

    are driven radially into the barrier layer and supported by the surrounding steel casing. In order to verify the integrity of the concept the seal system is subjected cyclic pressure and temperature variations to simulate the service conditions.The aim of the testing is to demonstrate the sensitivity...... of the seal system geometry and its tolerances necessary to maintain a tight seal. The test is carried out in a purpose built autoclave, in which the seal system can be tested while undergoing variations in pressure and temperature.The paper will present a study on the importance of the geometry of the gasket...... and the inner liner. The inner and outer diameter of the gasket are varied to see the effectiveness of the seal mechanism. The effect of varying the width of the gasket as well as the surface roughness of the components in the seal system is analysed. Finally, it is investigated how the seal system is affected...

  15. Suppression of the UV-sensitive phenotype of Escherichia coli recF mutants by recA(Srf) and recA(Tif) mutations requires recJ+

    International Nuclear Information System (INIS)

    Thoms, B.; Wackernagel, W.

    1988-01-01

    Mutations in recA, such as recA801(Srf) (suppressor of RecF) or recA441(Tif) (temperature-induced filamentation) partially suppress the deficiency in postreplication repair of UV damage conferred by recF mutations. We observed that spontaneous recA(Srf) mutants accumulated in cultures of recB recC sbcB sulA::Mu dX(Ap lac) lexA51 recF cells because they grew faster than the parental strain. We show that in a uvrA recB+ recC+ genetic background there are two prerequisites for the suppression by recA(Srf) of the UV-sensitive phenotype of recF mutants. (i) The recA(Srf) protein must be provided in increased amounts either by SOS derepression or by a recA operator-constitutive mutation in a lexA(Ind) (no induction of SOS functions) genetic background. (ii) The gene recJ, which has been shown previously to be involved in the recF pathway of recombination and repair, must be functional. The level of expression of recJ in a lexA(Ind) strain suffices for full suppression. Suppression by recA441 at 30 degrees C also depends on recJ+. The hampered induction by UV of the SOS gene uvrA seen in a recF mutant was improved by a recA(Srf) mutation. This improvement did not require recJ+. We suggest that recA(Srf) and recA(Tif) mutant proteins can operate in postreplication repair independent of recF by using the recJ+ function

  16. Joint seal in tank

    International Nuclear Information System (INIS)

    Colquhoun, J.; White, G.V.

    1981-01-01

    A seal for a joint or gap between edges of adjacent wall sections (e.g. of concrete) of a liquid-containing vessel, such as a nuclear reactor cooling pond, comprises a sheet metal strip having longitudinally-extending edge parts, secured to the respective vessel-section edges, and a central part which is longitudinally corrugated to provide sufficient flexibility to accommodate slight relative movements between the vessel-section edges (e.g. due to thermal expansions). The edges of the sheet metal of the strip are turned in so that the edge parts of the strip are formed as generally U-section channels. These accommodate longitudinally extending securing bars which are bolted to the vessel wall sections by bolts which pass through the bars, through the free-edged wall of the channel section and through a longitudinally extending resilient seal pad compressed between that wall of the channel section and the vessel wall section to which it is secured. The other wall of the channel section (integral with the corrugated central part of the strip) has access windows through which the bolts are inserted and tightened, the windows being then closed off in liquid-tight manner by welding closure caps over them. (author)

  17. Development of a sensitive determination method for benzotriazole UV stabilizers in enviromental water samples with stir bar sorption extraction and liquid desorption prior to ultra-high performance liquid chromatography with tandem mass spectrometry.

    Science.gov (United States)

    Montesdeoca-Esponda, Sarah; del Toro-Moreno, Adrián; Sosa-Ferrera, Zoraida; Santana-Rodríguez, José Juan

    2013-07-01

    Benzotriazole UV stabilizers are emerging compounds used in personal care products and can enter surface water after passing through wastewater treatment plants without being removed. Because these analytes are strongly hydrophobic, there is an environmental risk of accumulation in solid matrices and magnification through the trophic chain. In this work, a method based on stir bar sorption extraction with liquid desorption is presented for the extraction of benzotriazole UV stabilizers from water samples. Stir bar sorptive extraction was combined with ultra-high performance LC with MS/MS detection. All important factors affecting the stir bar sorptive extraction procedure are discussed, and the optimized method was applied to seawater and wastewater samples from Gran Canaria Island, providing good selectivity and sensitivity with LODs and limits of quantification in the range of 18.4-55.1 and 61.5-184 ng/L, respectively. Recoveries between 68.4-92.2% were achieved for the more polar compounds, whereas the recoveries were lower for the two less polar compounds, most likely due to their strong absorption into the polydimethylsiloxane stir bar phase that does not allows the complete desorption. The repeatability studies gave RSDs of between 6.45 and 12.6% for all compounds in the real samples. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. In Vitro Repair of UV-Irradiated Micrococcus luteus Bacteriophage N1 Transfecting DNA 1

    Science.gov (United States)

    Mahler, Inga; George, Jeanne; Grossman, Lawrence

    1974-01-01

    Calcium-treated UV-sensitive, host cell reactivation− strains of Micrococcus luteus are infected with UV-irradiated N1 DNA. In strains lacking UV endonuclease, in vitro treatment of the irradiated DNA results in transfection enhancement. PMID:4823319

  19. Repository Closure and Sealing Approach

    International Nuclear Information System (INIS)

    A.T. Watkins

    2000-01-01

    The scope of this analysis will be to develop the conceptual design of the closure seals and their locations in the Subsurface Facilities. The design will be based on the recently established program requirements for transitioning to the Site Recommendation (SR) design as outlined by ''Approach to Implementing the Site Recommendation Baseline'' (Stroupe 2000) and the ''Monitored Geologic Repository Project Description Document'' (CRWMS M andO 1999b). The objective of this analysis will be to assist in providing a description for the Subsurface Facilities System Description Document, Section 2 and finally to document any conclusions reached in order to contribute and provide support to the SR. This analysis is at a conceptual level and is considered adequate to support the SR design. The final closure barriers and seals for the ventilation shafts, and the north and south ramps will require these openings to be permanently sealed to limit excessive air and water inflows and prevent human intrusion. The major tasks identified with closure in this analysis are: (1) Developing the overall subsurface seal layout and identifying design and operational interfaces for the Subsurface Facilities. (2) Summarizing the general site conditions and general rock characteristic with respect to seal location and describing the seal selected. (3) Identify seal construction materials, methodology of construction and strategic locations including design of the seal and plugs. (4) Discussing methods to prevent human intrusion

  20. Face-Sealing Butterfly Valve

    Science.gov (United States)

    Tervo, John N.

    1992-01-01

    Valve plate made to translate as well as rotate. Valve opened and closed by turning shaft and lever. Interactions among lever, spring, valve plate, and face seal cause plate to undergo combination of translation and rotation so valve plate clears seal during parts of opening and closing motions.

  1. Research on seal control systems for international nuclear safeguard and the vulnerability assessment on the seals

    International Nuclear Information System (INIS)

    Zhang Hongjian; Liu Tianshu; Cao Fangfang; Xu Chunyan

    2014-01-01

    Safeguard seals, also called Tamper-indicating devices (TIDs), are widely used to detect tampering or unauthorized entry in the international safeguard and security systems, Seal control systems consist of seal implementing plan, seal development and the vulnerability assessment on tbe seals, effective implementing procedures and methods of the seals. The vulnerability assessment contents of safeguard seals, thermo-shrinked film seals being as an example, and seals control systems in the implementation program are researched. The seal control systems discuss task assignment, seals management flow and seals program data flow to promote applying effectively seals. The vulnerability assessment program of seals studies assurance level to some different tampering techniques and measures. The researches must promote utilizing seals effectively for nuclear security, non-proliferation of nuclear weapons, radioactive waste management, and the nuclear material accounting and control. (authors)

  2. Fast and sensitive HPLC/UV method for cefazolin quantification in plasma and subcutaneous tissue microdialysate of humans and rodents applied to pharmacokinetic studies in obese individuals.

    Science.gov (United States)

    Palma, Eduardo Celia; Laureano, João Victor; de Araújo, Bibiana Verlindo; Meinhardt, Nelson Guardiola; Stein, Airton Tetelbom; Dalla Costa, Teresa

    2018-04-14

    Antimicrobial prophylactic dosing of morbidly obese patients may differ from normal weighted individuals owing to alterations in drug tissue distribution. Drug subcutaneous tissue distribution can be investigated by microdialysis patients and animals. The need for cefazolin prophylactic dose adjustment in obese patients remains under discussion. The paper describes the validation of an HPLC-UV method for cefazolin quantification in plasma and microdialysate samples from clinical and pre-clinical studies. A C 18 column with an isocratic mobile phase was used for drug separation, with detection at 272 nm. Total and unbound cefazolin lower limit of quantitation was 5 μg/mL in human plasma, 2 μg/mL in rat plasma, and 0.5 and 0.025 μg/mL in human and rat microdialysate samples, respectively. The maximum intra- and inter-day imprecisions were 10.7 and 8.1%, respectively. The inaccuracy was <9.7%. The limit of quantitation imprecision and inaccuracy were < 15%. Cefazolin stability in the experimental conditions was confirmed. Cefazolin plasma concentrations and subcutaneous tissue penetration were determined by microdialysis in morbidly obese patients (2 g i.v. bolus) and diet-induced obese rats (30 mg/kg i.v. bolus) using the method. This method has the main advantages of easy plasma clean-up and practicability and has proven to be useful in cefazolin clinical and pre-clinical pharmacokinetic investigations. Copyright © 2018 John Wiley & Sons, Ltd.

  3. Airfoil seal system for gas turbine engine

    Science.gov (United States)

    None, None

    2013-06-25

    A turbine airfoil seal system of a turbine engine having a seal base with a plurality of seal strips extending therefrom for sealing gaps between rotational airfoils and adjacent stationary components. The seal strips may overlap each other and may be generally aligned with each other. The seal strips may flex during operation to further reduce the gap between the rotational airfoils and adjacent stationary components.

  4. High pressure mechanical seal

    Science.gov (United States)

    Babel, Henry W. (Inventor); Anderson, Raymond H. (Inventor)

    1996-01-01

    A relatively impervious mechanical seal is formed between the outer surface of a tube and the inside surface of a mechanical fitting of a high pressure fluid or hydraulic system by applying a very thin soft metal layer onto the outer surface of the hard metal tube and/or inner surface of the hard metal fitting. The thickness of such thin metal layer is independent of the size of the tube and/or fittings. Many metals and alloys of those metals exhibit the requisite softness, including silver, gold, tin, platinum, indium, rhodium and cadmium. Suitably, the coating is about 0.0025 millimeters (0.10 mils) in thickness. After compression, the tube and fitting combination exhibits very low leak rates on the order or 10.sup.-8 cubic centimeters per second or less as measured using the Helium leak test.

  5. Inhibitory effects of ambient levels of solar UV-A and UV-B radiation on growth of cucumber

    International Nuclear Information System (INIS)

    Krizek, D.T.; Mirecki, R.M.; Britz, S.J.

    1997-01-01

    The influence of solar UV-A and UV-B radiation at Beltsville, Maryland, on growth and flavonoid content in four cultivars of Cucumis sativus L. (Ashley, Poinsett, Marketmore, and Salad Bush cucumber) was examined during the summers of 1994 and 1995. Plants were grown from seed in UV exclusion chambers consisting of UV-transmitting Plexiglas, lined with Llumar to exclude UV-A and UV-B, polyester to exclude UV-B, or cellulose acetate to transmit UV-A and UV-B. Despite previously determined differences in sensitivity to supplemental UV-B radiation, all four cultivars responded similarly to UV-B exclusion treatment. After 19–21 days, the four cultivars grown in the absence of solar UV-B (polyester) had an average of 34, 55, and 40% greater biomass of leaves, stems, and roots, respectively, 27% greater stem height, and 35% greater leaf area than those grown under ambient UV-B (cellulose acetate). Plants protected from UV-A radiation as well (Llumar) showed an additional 14 and 22% average increase, respectively, in biomass of leaves and stems, and a 22 and 19% average increase, respectively, in stem elongation and leaf area over those grown under polyester. These findings demonstrate the extreme sensitivity of cucumber not only to present levels of UV-B but also to UV-A and suggest that even small changes in ozone depletion may have important biological consequences for certain plant species. (author)

  6. Turbine and Structural Seals Team Facilities

    Data.gov (United States)

    Federal Laboratory Consortium — Seals Team Facilities conceive, develop, and test advanced turbine seal concepts to increase efficiency and durability of turbine engines. Current projects include...

  7. Crack Growth Properties of Sealing Glasses

    Science.gov (United States)

    Salem, Jonathan A.; Tandon, R.

    2008-01-01

    The crack growth properties of several sealing glasses were measured using constant stress rate testing in 2% and 95% RH (relative humidity). Crack growth parameters measured in high humidity are systematically smaller (n and B) than those measured in low humidity, and velocities for dry environments are approx. 100x lower than for wet environments. The crack velocity is very sensitivity to small changes in RH at low RH. Confidence intervals on parameters that were estimated from propagation of errors were comparable to those from Monte Carlo simulation.

  8. Leak detection of KNI seals

    International Nuclear Information System (INIS)

    Baranyai, G.; Peter, A.; Windberg, P.

    1990-03-01

    In Unit 3 and 4 of the Paks Nuclear Power Plant, Hungary, KNI type seals are used as lead-throughs with conical nickel sealing rings. Their failure can be critical for the operation of the reactor. An Acoustical Leak Detection System (ALDS) was constructed and tested for the operational testing of the seals. Some individual papers are presented in this collection on the calibration and testing of the ALDS intended to be placed on the top of the reactor vessels. The papers include simulation measurements of Unit 3 of NPP, laboratory experiments, evaluation of measurements, and further development needs with the ALDS. (R.P.) 50 figs.; 19 tabs

  9. Silicone foam for penetration seal

    International Nuclear Information System (INIS)

    Hoshino, Yoshikazu

    1986-01-01

    In nuclear power plants or general buildings, it is very important to form a fire-resistant seal around cables, cable trays and conduits passing through a wall or a floor. Rockwool, asbestos, glasswool and flame-retarded urethane foam have so far been used for these purposes. However, they were not satisfactory in sealing property, workability and safety. The silicone foam newly developed, ''TOSSEAL'' 300, has cleared these defects. It has now come to be used for fire resistant seal in nuclear power plants. (author)

  10. Utilization of O4 Slant Column Density to Derive Aerosol Layer Height from a Space-Borne UV-Visible Hyperspectral Sensor: Sensitivity and Case Study

    Science.gov (United States)

    Park, Sang Seo; Kim, Jhoon; Lee, Hanlim; Torres, Omar; Lee, Kwang-Mog; Lee, Sang Deok

    2016-01-01

    The sensitivities of oxygen-dimer (O4) slant column densities (SCDs) to changes in aerosol layer height are investigated using the simulated radiances by a radiative transfer model, the linearized pseudo-spherical vector discrete ordinate radiative transfer (VLIDORT), and the differential optical absorption spectroscopy (DOAS) technique. The sensitivities of the O4 index (O4I), which is defined as dividing O4 SCD by 10(sup 40) molecules (sup 2) per centimeters(sup -5), to aerosol types and optical properties are also evaluated and compared. Among the O4 absorption bands at 340, 360, 380, and 477 nanometers, the O4 absorption band at 477 nanometers is found to be the most suitable to retrieve the aerosol effective height. However, the O4I at 477 nanometers is significantly influenced not only by the aerosol layer effective height but also by aerosol vertical profiles, optical properties including single scattering albedo (SSA), aerosol optical depth (AOD), particle size, and surface albedo. Overall, the error of the retrieved aerosol effective height is estimated to be 1276, 846, and 739 meters for dust, non-absorbing, and absorbing aerosol, respectively, assuming knowledge on the aerosol vertical distribution shape. Using radiance data from the Ozone Monitoring Instrument (OMI), a new algorithm is developed to derive the aerosol effective height over East Asia after the determination of the aerosol type and AOD from the MODerate resolution Imaging Spectroradiometer (MODIS). About 80 percent of retrieved aerosol effective heights are within the error range of 1 kilometer compared to those obtained from the Cloud-Aerosol Lidar with Orthogonal Polarization (CALIOP) measurements on thick aerosol layer cases.

  11. Utilization of O4 Slant Column Density to Derive Aerosol Layer Height from a Spaceborne UV-Visible Hyperspectral Sensor: Sensitivity and Case Study

    Science.gov (United States)

    Park, Sang Seo; Kim, Jhoon; Lee, Hanlim; Torres, Omar; Lee, Kwang-Mog; Lee, Sang Deok

    2016-01-01

    The sensitivities of oxygen-dimer (O4) slant column densities (SCDs) to changes in aerosol layer height are investigated using the simulated radiances by a radiative transfer model, the linearized pseudo-spherical vector discrete ordinate radiative transfer (VLIDORT), and the Differential Optical Absorption Spectroscopy (DOAS) technique. The sensitivities of the O4 index (O4I), which is defined as dividing O4 SCD by 10(exp 40) sq molecules cm(exp -5), to aerosol types and optical properties are also evaluated and compared. Among the O4 absorption bands at 340, 360, 380, and 477 nm, the O4 absorption band at 477 nm is found to be the most suitable to retrieve the aerosol effective height. However, the O4I at 477 nm is significantly influenced not only by the aerosol layer effective height but also by aerosol vertical profiles, optical properties including single scattering albedo (SSA), aerosol optical depth (AOD), particle size, and surface albedo. Overall, the error of the retrieved aerosol effective height is estimated to be 1276, 846, and 739 m for dust, non-absorbing, and absorbing aerosol, respectively, assuming knowledge on the aerosol vertical distribution shape. Using radiance data from the Ozone Monitoring Instrument (OMI), a new algorithm is developed to derive the aerosol effective height over East Asia after the determination of the aerosol type and AOD from the MODerate resolution Imaging Spectroradiometer (MODIS). About 80% of retrieved aerosol effective heights are within the error range of 1 km compared to those obtained from the Cloud-Aerosol Lidar with Orthogonal Polarization (CALIOP) measurements on thick aerosol layer cases.

  12. The numbers of ringed seals (Phoca hispida in Baffin Bay and associated waters

    Directory of Open Access Journals (Sweden)

    Michael C S Kingsley

    1998-06-01

    Full Text Available The size of the population of ringed seals (Phoca hispida inhabiting Baffin Bay and associated waters was estimated by two methods. An approximate model of the energetics of the polar bear (Ursus maritimus estimated an energetic need of about 16,000 MJ/bear per year. Modelled estimates of the energetic yield of a ringed seal population showed that a stable standing population of 140-170 ringed seals per bear would be needed to provide that much energy, assuming that all mortalities were due to polar bear predation. This result was sensitive to assumptions about the Field Metabolic Rate (FMR of the bears and the energetic yield of individual ringed seals, but less sensitive to assumptions about relative incidence of predation on different age classes of seal or the age structure of the polar bear population. Estimated sizes of polar bear populations in Baffin Bay and associated waters (total about 4,025, and of the standing population needed to support an estimated hunter kill of 100,000 yielded a population estimate of, very roughly, 1.2 million ringed seals. Estimates of ice areas and of the density of hauled out seals from aerial surveys were used to generate another approximate figure for the ringed seal population, which was about the same. The density of seals in the pack-ice area of Baffin Bay, which is imperfectly known, has a large influence on the latter estimate.

  13. Seal coat binder performance specifications.

    Science.gov (United States)

    2013-11-01

    Need to improve seal coat binder specs: replace empirical tests (penetration, ductility) with : performance-related tests applicable to both : unmodified and modified binders; consider temperatures that cover entire in service : range that are tied t...

  14. Alaska Harbor Seal Glacial Surveys

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Floating glacial ice serves as a haul-out substrate for a significant number (10-15%) of Alaskan harbor seals, and thus surveying tidewater glacial fjords is an...

  15. Coupled processes in repository sealing

    International Nuclear Information System (INIS)

    Case, J.B.; Kelsall, P.C.

    1985-01-01

    The significance of coupled processes in repository sealing is evaluated. In most repository designs, shaft seals will be located in areas of relatively low temperature perturbation, in which case the coupling of temperature with stress and permeability may be less significant than the coupling between stress and permeability that occurs during excavation. Constitutive relationships between stress and permeability are reviewed for crystalline rock and rocksalt. These provide a basis for predicting the development of disturbed zones near excavations. Field case histories of the degree of disturbance are presented for two contrasting rock types - Stripa granite and Southeastern New Mexico rocksalt. The results of field investigations in both rock types confirm that hydraulic conductivity or permeability is stress dependent, and that shaft seal performance may be related to the degree that stresses are perturbed and restored near the seal

  16. Northern Fur Seal Food Habits

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains food habits samples, usually scats, collected opportunistically on northern fur seal rookeries and haulouts in Alaska from 1987 to present....

  17. Design considerations for mechanical face seals

    Science.gov (United States)

    Ludwig, L. P.; Greiner, H. F.

    1980-01-01

    Two companion reports deal with design considerations for improving performance of mechanical face seals, one of family of devices used in general area of fluid sealing of rotating shafts. One report deals with basic seal configuration and other with lubrication of seal.

  18. Seals Research at AlliedSignal

    Science.gov (United States)

    Ullah, M. Rifat

    1996-01-01

    A consortium has been formed to address seal problems in the Aerospace sector of Allied Signal, Inc. The consortium is represented by makers of Propulsion Engines, Auxiliary Power Units, Gas Turbine Starters, etc. The goal is to improve Face Seal reliability, since Face Seals have become reliability drivers in many of our product lines. Several research programs are being implemented simultaneously this year. They include: Face Seal Modeling and Analysis Methodology; Oil Cooling of Seals; Seal Tracking Dynamics; Coking Formation & Prevention; and Seal Reliability Methods.

  19. DNA damage caused by UV- and near UV-irradiation

    International Nuclear Information System (INIS)

    Ohnishi, Takeo

    1986-01-01

    Much work with mutants deficient in DNA repair has been performed concerning UV-induced DNA damage under the condition where there is no artificial stimulation. In an attempt to infer the effects of solar wavelengths, the outcome of the work is discussed in terms of cellular radiation sensitivity, unscheduled DNA synthesis, and mutation induction, leading to the conclusion that some DNA damage occurs even by irradiation of the shorter wavelength light (270 - 315 nm) and is repaired by excision repair. It has been thought to date that pyrimidine dimer (PD) plays the most important role in UV-induced DNA damage, followed by (6 - 4) photoproducts. As for DNA damage induced by near UV irradiation, the yield of DNA single-strand breaks and of DNA-protein crosslinking, other than PD, is considered. The DNA-protein crosslinking has proved to be induced by irradiation at any wavelength of UV ranging from 260 to 425 nm. Near UV irradiation causes the inhibition of cell proliferation to take place. (Namekawa, K.)

  20. Sealing Failure Analysis on V-Shaped Sealing Rings of an Inserted Sealing Tool Used for Multistage Fracturing Processes

    Directory of Open Access Journals (Sweden)

    Gang Hu

    2018-06-01

    Full Text Available The inserted sealing tool is a critical downhole implement that is used to balance the downhole pressure in multistage fracturing operations and prevent fracturing fluid from overflow and/or backward flow. The sealing ring of an inserted sealing tool plays an important role in downhole sealing since a sealing failure would ail the fracturing operation. In order to improve the sealing performance and reduce the potential fracturing failures, this research aims to investigate the influence of V-shaped sealing ring geometries on sealing performance. Constitutive experiments of rubber materials were carried out and the parameters of the constitutive relationship of rubber materials were obtained. A two-dimensional axisymmetric model considering the sealing ring has been established and influences are investigated with considerations of various system parameters and operating conditions. It is found that the stresses concentrated at the shoulder and inner vertex of the sealing ring have direct impact on the damage of the sealing rings under operational conditions. Moreover, the sealing interference, among several other factors, greatly affects the life of the sealing ring. A new design of the sealing ring is suggested with optimized geometric parameters. Its geometric parameters are the edge height of 5 mm, the vertex angle of 90°–100°, and the interference of 0.1 mm, which show a better performance and prolonged operation life of the sealing ring.

  1. Sealed can of spent fuel

    International Nuclear Information System (INIS)

    Suzuki, Yasuyuki.

    1976-01-01

    Object: To provide a seal plug cover with a gripping portion fitted to a canning machine and a gripping portion fitted to a gripper of the same configuration as a fuel body for handling the fuel body so as to facilitate the handling work. Structure: A sealed can comprises a vessel and a seal plug cover, said cover being substantially in the form of a bottomed cylinder, which is slipped on the vessel and air-tightly secured by a fastening bolt between it and a flange. The spent fuel body is received into the vessel together with coolant during the step of canning operation. Said seal plug cover has two gripping portions, one for opening and closing the plug cover of the canning machine as an exclusive use member, the other being in the form of a hook-shaped peripheral groove, whereby the gripping portions may be effectively used using the same gripper when the spent fuel body is transported while being received in the sealed can or when the fuel body is removed from the sealed can. (Kawakami, Y.)

  2. Reusable, tamper-indicating seal

    International Nuclear Information System (INIS)

    Ryan, M.J.

    1978-01-01

    A reusable, tamper-indicating seal is comprised of a drum confined within a fixed body and rotatable in one direction therewithin, the top of the drum constituting a tray carrying a large number of small balls of several different colors. The fixed body contains parallel holes for looping a seal wire therethrough. The base of the drums carries cams adapted to coact with cam followers to lock the wire within the seal at one angular position of the drum. A channel in the fixed body, visible from outside the seal, adjacent the tray constitutes a segregated location for a small plurality of the colored balls. A spring in the tray forces colored balls into the segregated location at one angular position of the drum, further rotation securing the balls in position and the wires in the seal. A wedge-shaped plough removes the balls from the segregated location, at a different angular position of the drum, the wire being unlocked at the same postion. A new pattern of colored balls will appear in the segregated location when the seal is relocked

  3. Labyrinth Seal Flutter Analysis and Test Validation in Support of Robust Rocket Engine Design

    Science.gov (United States)

    El-Aini, Yehia; Park, John; Frady, Greg; Nesman, Tom

    2010-01-01

    High energy-density turbomachines, like the SSME turbopumps, utilize labyrinth seals, also referred to as knife-edge seals, to control leakage flow. The pressure drop for such seals is order of magnitude higher than comparable jet engine seals. This is aggravated by the requirement of tight clearances resulting in possible unfavorable fluid-structure interaction of the seal system (seal flutter). To demonstrate these characteristics, a benchmark case of a High Pressure Oxygen Turbopump (HPOTP) outlet Labyrinth seal was studied in detail. First, an analytical assessment of the seal stability was conducted using a Pratt & Whitney legacy seal flutter code. Sensitivity parameters including pressure drop, rotor-to-stator running clearances and cavity volumes were examined and modeling strategies established. Second, a concurrent experimental investigation was undertaken to validate the stability of the seal at the equivalent operating conditions of the pump. Actual pump hardware was used to construct the test rig, also referred to as the (Flutter Rig). The flutter rig did not include rotational effects or temperature. However, the use of Hydrogen gas at high inlet pressure provided good representation of the critical parameters affecting flutter especially the speed of sound. The flutter code predictions showed consistent trends in good agreement with the experimental data. The rig test program produced a stability threshold empirical parameter that separated operation with and without flutter. This empirical parameter was used to establish the seal build clearances to avoid flutter while providing the required cooling flow metering. The calibrated flutter code along with the empirical flutter parameter was used to redesign the baseline seal resulting in a flutter-free robust configuration. Provisions for incorporation of mechanical damping devices were introduced in the redesigned seal to ensure added robustness

  4. Effects of UV radiation on freshwater metazooplankton

    International Nuclear Information System (INIS)

    Tartarotti, B.

    1999-06-01

    There is evidence that fluxes of solar ultraviolet-B radiation (UV-B, 290-320 nm) are increasing over wide parts of the earth's surface due to stratospheric ozone depletion. UV radiation (290-400 nm) can have damaging effects on biomolecules and cell components that are common to most living organisms. The aim of this thesis is to gain a more thorough understanding of the potential impacts of solar radiation on freshwater metazooplankton. To detect UV-vulnerability in zooplankton populations dominating the zooplankton community of two clear-water, high mountain lakes located one in the Austrian Alps and another in the Chilean Andes, the survival of two copepod species was studied. The organisms were exposed to a 10- to 100-fold increase in UV-B radiation compared to those levels found at their natural, maximum daytime distribution. Both species vertically migrate and are pigmented. UV-absorbing compounds with a maximum absorption at ∼334 nm were also detected. Cyclops abyssorum tatricus, a common cyclopoid copepod species of Alpine lakes, was highly resistant to UV-B radiation and no significant lethal effect was observed. The calanoid copepod Boeckella gracilipes, frequent in Andean lakes, had a mortality ∼5 times higher in the treatment receiving full sunlight than in the UV-B excluded treatment (3.2 %) only when exposed for 70 h. The resistance of B. gracilipes was higher than that reported in the literature for the same species suggesting the existence of intraspecific differences in UV sensitivity. Survival, fecundity and development of the zooplankton community of a clear-water, high elevation Andean lake (33 o S) were studied with mesocosms experiments after prolonged UV exposure (48 days). When exposed to full sunlight, the population of the cladoceran Chydorus sphaericus and the rotifer Lepadella ovalis were strongly inhibited by UV-B, whereas both species were resistant to UV-A radiation. Conversely, UV-B radiation had no effect on the survival of the

  5. Mutational synergism between p-fluorophenylalaline and UV in Coprinus lagopus

    International Nuclear Information System (INIS)

    Talmud, P.J.

    1977-01-01

    The amino acid analogue p-fluorophenylalanine (PFP) is mutagenic to Coprinus lagopus due to its incorporation into proteins. Spontaneous mutations, PFP and UV mutagenesis and PFP/UV synergism have been studied in a UV resistant strain and in two complementing UV sensitive mutant strains. By comparison to the UV resistant strain, one UV sensitive strain shows normal spontaneous mutations, 1.4% PFP-induced mutations and 50-fold UV mutagenesis. The second UV sensitive strain has 19-fold spontaneous mutation frequency and slightly elevated UV mutagenesis. In all 3 strains the PFP/UV synergism is comparable (4-5 times the arithmetic expected). The results indicate that PFP mutagenesis is due to the incorporation of PFP into enzymes normally functioning in the organism but which also participate in UV repair mechanisms. A model is proposed for UV repair which is based on a PFP sensitive excision repair system of at least two enzymes, an alternative 'error-proof' pathway which is not susceptible to PFP and an 'error-prone' pathway which is responsible for UV mutagenesis and is susceptible to PFP as shown by the PFP/UV synergism. Because PFP is given before UV treatment, this implies a UV inducible cofactor and a PFP sensitive enzyme which only functions after UV activation

  6. Spray sealing: A breakthrough in integral fuel tank sealing technology

    Science.gov (United States)

    Richardson, Martin D.; Zadarnowski, J. H.

    1989-11-01

    In a continuing effort to increase readiness, a new approach to sealing integral fuel tanks is being developed. The technique seals potential leak sources by spraying elastomeric materials inside the tank cavity. Laboratory evaluations project an increase in aircraft supportability and reliability, an improved maintainability, decreasing acquisition and life cycle costs. Increased usable fuel volume and lower weight than conventional bladders improve performance. Concept feasibility was demonstrated on sub-scale aircraft fuel tanks. Materials were selected by testing sprayable elastomers in a fuel tank environment. Chemical stability, mechanical properties, and dynamic durability of the elastomer are being evaluated at the laboratory level and in sub-scale and full scale aircraft component fatigue tests. The self sealing capability of sprayable materials is also under development. Ballistic tests show an improved aircraft survivability, due in part to the elastomer's mechanical properties and its ability to damp vibrations. New application equipment, system removal, and repair methods are being investigated.

  7. Sensitizing pigment in the fly

    International Nuclear Information System (INIS)

    Vogt, K.; Kirschfeld, K.

    1983-01-01

    The sensitizing pigment hypothesis for the high UV sensitivity in fly photoreceptors (R1-6) is further substantiated by measurements of the polarisation sensitivity in the UV. The quantum yield of the energy transfer from sensitizing pigment to rhodopsin was estimated by electrophysiological measurements of the UV sensitivity and the rhabdomeric absorptance (at 490 nm) in individual receptor cells. The transfer efficiency is >=0.75 in receptors with an absorptance in the rhabdomeres of 0.55-0.95. This result suggests that the sensitizing pigment is bound in some way to the rhodopsin. A ratio of two molecules of sensitizing pigment per one rhodopsin is proposed. (orig.)

  8. Seal Related Development Activities at EG/G

    Science.gov (United States)

    Greiner, Harold F.

    1991-01-01

    Seal related development activities including modeling, analysis, and performance testing are described for several current seal related projects. Among the current seal related projects are the following: high pressure gas sealing systems for turbomachinery; brush seals for gas path sealing in gas turbines; and tribological material evaluation for wear surfaces in sealing systems.

  9. Radioactive material package seal tests

    International Nuclear Information System (INIS)

    Madsen, M.M.; Humphreys, D.L.; Edwards, K.R.

    1990-01-01

    General design or test performance requirements for radioactive materials (RAM) packages are specified in Title 10 of the US Code of Federal Regulations Part 71 (US Nuclear Regulatory Commission, 1983). The requirements for Type B packages provide a broad range of environments under which the system must contain the RAM without posing a threat to health or property. Seals that provide the containment system interface between the packaging body and the closure must function in both high- and low-temperature environments under dynamic and static conditions. A seal technology program, jointly funded by the US Department of Energy Office of Environmental Restoration and Waste Management (EM) and the Office of Civilian Radioactive Waste Management (OCRWM), was initiated at Sandia National Laboratories. Experiments were performed in this program to characterize the behavior of several static seal materials at low temperatures. Helium leak tests on face seals were used to compare the materials. Materials tested include butyl, neoprene, ethylene propylene, fluorosilicone, silicone, Eypel, Kalrez, Teflon, fluorocarbon, and Teflon/silicone composites. Because most elastomer O-ring applications are for hydraulic systems, manufacturer low-temperature ratings are based on methods that simulate this use. The seal materials tested in this program with a fixture similar to a RAM cask closure, with the exception of silicone S613-60, are not leak tight (1.0 x 10 -7 std cm 3 /s) at manufacturer low-temperature ratings. 8 refs., 3 figs., 1 tab

  10. Simulation of the noise transmission through automotive door seals

    CERN Document Server

    Hazir, Andreas

    2016-01-01

    Andreas Hazir is investigating the door seal contribution to the interior noise level of production vehicles. These investigations contain experimental contribution analyses of real production vehicles and of academic test cases as well as the development of a simulation methodology for noise transmission through sealing systems and side windows. The simulations are realized by coupling transient computational aeroacoustics of the exterior flow to nonlinear finite element simulations of the structural transmission. By introducing a linear transmission model, the setup and computational costs of the seal noise transmission are significantly reduced, resulting in the feasibility of numerical contribution analyses of real production vehicles. Contents Contribution Analyses of Production Vehicles Acoustic Excitation versus Aeroacoustic Excitation Development of a Simulation Methodology Sensitivity Analysis of Noise Transmission Simulations Target Groups Researchers and students in the field of automotive engineer...

  11. Mechanical seal with textured sidewall

    Energy Technology Data Exchange (ETDEWEB)

    Khonsari, Michael M.; Xiao, Nian

    2017-02-14

    The present invention discloses a mating ring, a primary ring, and associated mechanical seal having superior heat transfer and wear characteristics. According to an exemplary embodiment of the present invention, one or more dimples are formed onto the cylindrical outer surface of a mating ring sidewall and/or a primary ring sidewall. A stationary mating ring for a mechanical seal assembly is disclosed. Such a mating ring comprises an annular body having a central axis and a sealing face, wherein a plurality of dimples are formed into the outer circumferential surface of the annular body such that the exposed circumferential surface area of the annular body is increased. The texture added to the sidewall of the mating ring yields superior heat transfer and wear characteristics.

  12. Turbine interstage seal with self-balancing capability

    Science.gov (United States)

    Mills, Jacob A; Jones, Russell B; Sexton, Thomas D

    2017-09-26

    An interstage seal for a turbine of a gas turbine engine, the interstage seal having a seal carrier with an axial extending seal tooth movable with a stator of the engine, and a rotor with a seal surface that forms the interstage seal with the seal tooth, where a magnetic force produced by two magnets and a gas force produced by a gas pressure acting on the seal carrier forms a balancing force to maintain a close clearance of the seal without the seal tooth contacting the rotor seal surfaces during engine operation. In other embodiments, two pairs of magnets produce first and second magnetic forces that balance the seal in the engine.

  13. EBR-II rotating plug seal maintenance

    International Nuclear Information System (INIS)

    Allen, K.J.

    1986-01-01

    The EBR-II rotating plug seals require frequent cleaning and maintenance to keep the plugs from sticking during fuel handling. Time consuming cleaning on the cover gas and air sides of the dip ring seal is required to remove oxidation and sodium reaction products that accumulate and stop plug rotation. Despite severely limited access, effective seal cleaning techniques have removed 11 800 lb (5 352 kg) of deposits from the seals since 1964. Temperature control modifications and repairs have also required major maintenance work. Suggested seal design recommendations could significantly reduce maintenance on future similar seals

  14. Self-acting and hydrodynamic shaft seals

    Science.gov (United States)

    Ludwig, L. P.

    1973-01-01

    Self-acting and hydrodynamic seals are described. The analytical procedures are outlined for obtaining a seal force balance and the operating film thickness. Particular attention is given to primary ring response (seal vibration) to rotating seat face runout. This response analysis revealed three different vibration modes. Proposed applications of self-acting seals in gas turbine engines and in rocket vehicle turbopumps are described. Also experimental data on self-acting face seals operating under simulated gas turbine conditions are given; these data show the feasibility of operating the seal at conditions of 345 newtons per square centimeter (500 psi) and 152 meters per second (500 ft/sec) sliding speed.

  15. Sealed source peer review plan

    International Nuclear Information System (INIS)

    Feldman, Alexander; Leonard, Lee; Burns, Ron

    2009-01-01

    Sealed sources are known quantities of radioactive materials that have been encapsulated in quantities that produce known radiation fields. Sealed sources have multiple uses ranging from instrument calibration sources to sources that produce radiation fields for experimental applications. The Off-Site Source Recovery (OSR) Project at Los Alamos National Laboratory (LANL), created in 1999, under the direction of the Waste Management Division of the U.S. Department of Energy (DOE) Albuquerque has been assigned the responsibility to recover and manage excess and unwanted radioactive sealed sources from the public and private sector. LANL intends to ship drums containing qualified sealed sources to the Waste Isolation Pilot Plant (WIPP) for disposal. Prior to shipping, these drums must be characterized with respect to radiological content and other parameters. The U. S. Environmental Protection Agency (EPA) requires that ten radionulcides be quantified and reported for every container of waste to be disposed in the WIPP. The methods traditionally approved by the EPA include non-destructive assay (NDA) in accordance with Appendix A of the Contact-Handled Transuranic Waste Acceptance Criteria for the Waste Isolation Pilot Plant (DOE, 2002) (CH WAC). However, because of the nature and pedigree of historical records for sealed sources and the technical infeasibility of performing NDA on these sources, LANL proposes to characterize the content of these waste drums using qualified existing radiological data in lieu of direct measurement. This plan describes the process and documentation requirements for the use of the peer review process to qualify existing data for sealed radiological sources in lieu of perfonning radioassay. The peer review process will be performed in accordance with criteria provided in 40 CFR (section) 194.22 which specifies the use of the NUREG 1297 guidelines. The plan defines the management approach, resources, schedule, and technical requirements

  16. Genetics Home Reference: UV-sensitive syndrome

    Science.gov (United States)

    ... rays in sunlight. Even a small amount of sun exposure can cause a sunburn in affected individuals. In addition, these individuals can ... Resources (2 links) Merck Manual Home Health Edition: Sunburn World Health Organization: Sun Protection General Information from ...

  17. Índice UV

    Science.gov (United States)

    Información general sobre el Índice UV que proporciona un pronóstico del riesgo esperado de sobreexposición a la radiación ultravioleta (UV) del sol. El índice UV va acompañado de recomendaciones para protegerse del sol.

  18. uv dye lasers

    International Nuclear Information System (INIS)

    Abakumov, G.A.; Fadeev, V.V.; Khokhlov, R.V.; Simonov, A.P.

    1975-01-01

    The most important property of visible dye lasers, that is, continuous wavelength tuning, stimulated the search for dyes capable to lase in uv. They were found in 1968. Now the need for tunable uv lasers for applications in spectroscopy, photochemistry, isotope separation, remote air and sea probing, etc. is clearly seen. A review of some recent advances in uv dye lasers is reviewed

  19. Knife-edge seal for vacuum bagging

    Science.gov (United States)

    Rauschl, J. A.

    1980-01-01

    Cam actuated clamps pinch bagging material between long knife edge (mounted to clamps) and high temperature rubber cushion bonded to baseplate. No adhesive, tape, or sealing groove is needed to seal edge of bagging sheet against base plate.

  20. HMSRP Hawaiian Monk Seal Crittercam video

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This project investigates foraging behavior of Hawaiian monk seals by conducting telemetry studies. During these studies, live seals are instrumented with dive...

  1. Film riding seals for rotary machines

    Science.gov (United States)

    Bidkar, Rahul Anil; Sarawate, Neelesh Nandkumar; Wolfe, Christopher Edward; Ruggiero, Eric John; Raj Mohan, Vivek Raja

    2017-03-07

    A seal assembly for a rotary machine is provided. The seal assembly includes multiple sealing device segments disposed circumferentially intermediate to a stationary housing and a rotor. Each of the segments includes a shoe plate with a forward-shoe section and an aft-shoe section having one or more labyrinth teeth therebetween facing the rotor. The sealing device includes a stator interface element having a groove or slot for allowing disposal of a spline seal for preventing segment leakages. The sealing device segment also includes multiple bellow springs or flexures connected to the shoe plate and to the stator interface element. Further, the sealing device segments include a secondary seal integrated with the stator interface element at one end and positioned about the multiple bellow springs or flexures and the shoe plate at the other end.

  2. Legacy HMSRP Hawaiian Monk Seal Ultrasound Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Ultrasound images for measuring the condition of juvenile seals at Laysan Island during 2009-2010, collected when seals were handled as part of the De-Worming Project

  3. HMSRP Hawaiian Monk Seal Telemetry Tag Deployments

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This project investigates foraging behavior of Hawaiian monk seals by conducting telemetry studies. During these studies, live seals are instrumented with dive...

  4. Legacy HMSRP Hawaiian Monk Seals Observers

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set documents observers that have collected monk seal data as part of the ongoing monk seal population assessment efforts by PSD personnel and cooperating...

  5. HMSRP Hawaiian Monk Seal Handling Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains records for all handling and measurement of Hawaiian monk seals since 1981. Live seals are handled and measured during a variety of events...

  6. Recent advances in magnetic liquid sealing

    International Nuclear Information System (INIS)

    Raj, K.; Stahl, P.; Bottenberg, W.; True, D.; Martis, G.; Zook, C.

    1979-01-01

    In this paper recent work in design and testing of two special magnetic liquid seals extending the state-of-the-art of ferrofluidic sealing is discussed. These custom seals are a moving belt edge seal and an exclusion seal. The first seal provides a hermetic barrier to solid particulates expected to be present in enclosed nuclear environments. The second seal is used on a magnetic disk drive spindle and reduces the particulate contaminants in the memory disk pack area by up to three orders of magnitude. In addition, bearing life in the spindle is found to be doubled due to reduction of operating temperature. The fundamentals of magnetic fluid sealing are presented in terms of magnetic circuit design and physical properties of ferrofluids

  7. HMSRP Hawaiian Monk Seal Argos Location Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This project investigates foraging behavior of Hawaiian monk seals by conducting telemetry studies. During these studies, live seals are instrumented with satellite...

  8. HMSRP Hawaiian Monk Seal Entanglement data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The data set contains records of all entanglements of Hawaiian monk seals in marine debris. The data set comprises records of seals entangled by derelict fishing...

  9. Seal Apparatus and Methods to Manufacture Thereof

    Science.gov (United States)

    Richard, James A. (Inventor)

    2013-01-01

    In some implementations, apparatus and methods are provided through which a dynamic cryogenic seal is manufactured. In some implementations, the seal includes a retainer and a spring-seal assembly, the assembly being comprised of a main spring housing and fluorine-containing polymer seals. In some implementations, either a radial seal, or an axial (or "piston seal") is provided. In some implementations, methods of manufacturing the dynamic cryogenic seal are also provided. In some implementations, the methods include assembling the components while either heated or cooled, taking advantage of thermal expansion and contraction, such that there is a strong interference fit between the components at room temperature. In some implementations, this process ensures that the weaker fluorine-containing polymer seal is forced to expand and contract with the stronger retainer and spring and is under constant preload. In some implementations, the fluorine-containing polymer is therefore fluidized and retained, and can not lift off.

  10. Pre-sealing risk analysis

    International Nuclear Information System (INIS)

    Ensminger, D.A.; Hough, M.E.; Oston, S.G.

    1980-01-01

    This report describes studies of accidents involving high-level radioactive waste before sealing the waste into a repository. The report summarizes work done in this area during Fiscal Year 1978 and supplements previous work. Models of accident probability, severity, and consequences are refined and extended

  11. Dampers for Stationary Labyrinth Seals

    Science.gov (United States)

    El-Aini, Yehia; Mitchell, William; Roberts, Lawrence; Montgomery, Stuart; Davis, Gary

    2011-01-01

    Vibration dampers have been invented that are incorporated as components within the stationary labyrinth seal assembly. These dampers are intended to supplement other vibration-suppressing features of labyrinth seals in order to reduce the incidence of high-cycle-fatigue failures, which have been known to occur in the severe vibratory environments of jet engines and turbopumps in which labyrinth seals are typically used. A vibration damper of this type includes several leaf springs and/or a number of metallic particles (shot) all held in an annular seal cavity by a retaining ring. The leaf springs are made of a spring steel alloy chosen, in conjunction with design parameters, to maintain sufficient preload to ensure effectiveness of damping at desired operating temperatures. The cavity is vented via a small radial gap between the retaining ring and seal housing. The damping mechanism is complex. In the case of leaf springs, the mechanism is mainly friction in the slippage between the seal housing and individual dampers. In the case of a damper that contains shot, the damping mechanism includes contributions from friction between individual particles, friction between particles and cavity walls, and dissipation of kinetic energy of impact. The basic concept of particle/shot vibration dampers has been published previously; what is new here is the use of such dampers to suppress traveling-wave vibrations in labyrinth seals. Damping effectiveness depends on many parameters, including, but not limited to, coefficient of friction, mode shape, and frequency and amplitude of vibrational modes. In tests, preloads of the order of 6 to 15 lb (2.72 to 6.8 kilograms) per spring damper were demonstrated to provide adequate damping levels. Effectiveness of shot damping of vibrations having amplitudes from 20 to 200 times normal terrestrial gravitational acceleration (196 to 1,960 meters per square second) and frequencies up to 12 kHz was demonstrated for shot sizes from 0.032 to

  12. Reactor coolant pump seals: improving their performance

    International Nuclear Information System (INIS)

    Pothier, N.E.; Metcalfe, R.

    1986-06-01

    Large CANDU plants are benefitting from transient-resistant four-year reliable reactor coolant pump seal lifetimes, a direct result of AECL's 20-year comprehensive seal improvement program involving R and D staff, manufacturers, and plant designers and operators. An overview of this program is presented, which covers seal modification design, testing, post-service examination, specialized maintenance and quality control. The relevancy of this technology to Light Water Reactor Coolant Pump Seals is also discussed

  13. Development of simplified rotating plug seal structure

    International Nuclear Information System (INIS)

    Ueta, M.; Ichimiya, M.; Kanaoka, T.; Sekiya, H.; Ueda, S.; Ishibashi, S.

    1991-01-01

    We studied a compact and simplified rotating plug seal structure and conducted experiments for key elements of the concept such us the mechanical seal structure and sodium deposit prevention system. Good characteristics were confirmed for the mechanical seal structure, which utilizes an elastomer seal and thin lathe bearing. Applicability of the density barrier concept was also confirmed as the sodium deposit prevention system. This concept can be applied to actual plants. (author)

  14. Tamper-indicating devices and safeguards seals evaluation test report. Volume 2

    Energy Technology Data Exchange (ETDEWEB)

    Horton, P.R.V.; Waddoups, I.G.

    1995-08-01

    Volume I was based on a survey and an evaluation of seals that are used as tamper-indicating devices at DOE facilities. For that evaluation, currently available seals were physically and environmentally evaluated under two broad categories: handling durability and tamper resistance. Our study indicated that the environmental testing had no negative effects on the results of the mechanical tests. In Volume II, we evaluate some loop, fiber optic loop, and pressure-sensitive seals that are not used at DOE facilities. However, we continue to focus on qualities required by DOE: durability and tamper resistance. The seals are comparatively rated, and recommendations are made for using currently available seals and new tamper-indicating device technology.

  15. Tamper-indicating devices and safeguards seals evaluation test report. Volume 2

    International Nuclear Information System (INIS)

    Horton, P.R.V.; Waddoups, I.G.

    1995-08-01

    Volume I was based on a survey and an evaluation of seals that are used as tamper-indicating devices at DOE facilities. For that evaluation, currently available seals were physically and environmentally evaluated under two broad categories: handling durability and tamper resistance. Our study indicated that the environmental testing had no negative effects on the results of the mechanical tests. In Volume II, we evaluate some loop, fiber optic loop, and pressure-sensitive seals that are not used at DOE facilities. However, we continue to focus on qualities required by DOE: durability and tamper resistance. The seals are comparatively rated, and recommendations are made for using currently available seals and new tamper-indicating device technology

  16. Piston rod seal for a Stirling engine

    Science.gov (United States)

    Shapiro, Wilbur

    1984-01-01

    In a piston rod seal for a Stirling engine, a hydrostatic bearing and differential pressure regulating valve are utilized to provide for a low pressure differential across a rubbing seal between the hydrogen and oil so as to reduce wear on the seal.

  17. 19 CFR 113.25 - Seals.

    Science.gov (United States)

    2010-04-01

    ... signatures of principal and surety, if individuals, and the corporate seal shall be affixed close to the... law of the state in which executed. However, when the charter or governing statute of a corporation requires its acts to be evidenced by its corporate seal, such seal is required. ...

  18. Ergonomics and safety of manual bag sealing.

    NARCIS (Netherlands)

    Groot, M.D. de; Bosch, T.; Eikhout, S.M.; Vink, P.

    2005-01-01

    A variety of seals is used to close bags. Each seal has advantages and disadvantages. For shop assistants sealing bags could be a repetitive physically demanding action. Opening and closing the bags again can cause some discomfort or annoyance for consumers. Besides, it is an activity which can

  19. Improved sealing for in-core systems

    International Nuclear Information System (INIS)

    Dunford, S.

    1989-01-01

    The in-core instrumentation sealing nozzles designed by Framatome have three mechanical seals in series instead of the one traditional seal, and are pressurized by simply tightening up the nozzle covers. They have been installed from the start on all Framatome PWRs, as well as having been backfitted on Belgium and Yugoslavian units and chosen for the Chinese Qinshan plant. (author)

  20. Comparative study on two different seal surface structure for reactor pressure vessel sealing behavior

    International Nuclear Information System (INIS)

    Chen Jun; Xiong Guangming; Deng Xiaoyun

    2014-01-01

    The seal surface structure is very important to reactor pressure vessel (RPV) sealing behavior. In this paper, two 3-D RPV sealing analysis finite models have been established with different seal surface structures, in order to study the influence of two structures. The separation of RPV upper and lower flanges, bolt loads and etc. are obtained, which are used to evaluate the sealing behavior of the RPV. Meanwhile, the comparative analysis of safety margin of two seal surface structural had been done, which provides the theoretical basis for RPV seal structure design optimization. (authors)

  1. Sealing performance of a magnetic fluid seal for rotary blood pumps.

    Science.gov (United States)

    Mitamura, Yoshinori; Takahashi, Sayaka; Kano, Kentaro; Okamoto, Eiji; Murabayashi, Shun; Nishimura, Ikuya; Higuchi, Taka-Aki

    2009-09-01

    A magnetic fluid (MF) for a rotary blood pump seal enables mechanical contact-free rotation of the shaft and, hence, has excellent durability. The performance of a MF seal, however, has been reported to decrease in liquids. We have developed a MF seal that has a "shield" mechanism and a new MF with a higher magnetization of 47.9 kA/m. The sealing performance of the MF seal installed in a rotary blood pump was studied. Under the condition of continuous flow, the MF seal remained in perfect condition against a pressure of 298 mm Hg (pump flow rate: 3.96 L/min). The seal was also perfect against a pressure of 170 mm Hg in a continuous flow of 3.9 L/min for 275 days. We have developed a MF seal that works in liquid against clinically used pressures. The MF seal is promising as a shaft seal for rotary blood pumps.

  2. An Electrochromatography Chip with Integrated Waveguides for UV Absorbance Detection

    DEFF Research Database (Denmark)

    Gustafsson, Omar; Mogensen, Klaus Bo; Ohlsson, Pelle Daniel

    2008-01-01

    A silicon-based microchip for electrochromatographic separations is presented. Apart from a microfluidic network, the microchip has integrated UV-transparent waveguides for detection and integrated couplers for optical fibers on the chip, yielding the most complete chromatography microchip to date...... to the waveguides. The entire oxidized silicon microchip structure is sealed with a glass lid. Reversed phase electrochromatographic separation of three neutral compounds is demonstrated using UV absorbance detection at 254 nm. Baseline separation of the analytes is achieved in less than two minutes....

  3. Amphibious hearing in spotted seals (Phoca largha): underwater audiograms, aerial audiograms and critical ratio measurements.

    Science.gov (United States)

    Sills, Jillian M; Southall, Brandon L; Reichmuth, Colleen

    2014-03-01

    Spotted seals (Phoca largha) inhabit Arctic regions that are facing both rapid climate change and increasing industrialization. While little is known about their sensory capabilities, available knowledge suggests that spotted seals and other ice seals use sound to obtain information from the surrounding environment. To quantitatively assess their auditory capabilities, the hearing of two young spotted seals was tested using a psychophysical paradigm. Absolute detection thresholds for tonal sounds were measured in air and under water over the frequency range of hearing, and critical ratios were determined using octave-band masking noise in both media. The behavioral audiograms show a range of best sensitivity spanning four octaves in air, from approximately 0.6 to 11 kHz. The range of sensitive hearing extends across seven octaves in water, with lowest thresholds between 0.3 and 56 kHz. Critical ratio measurements were similar in air and water and increased monotonically from 12 dB at 0.1 kHz to 30 dB at 25.6 kHz, indicating that the auditory systems of these seals are quite efficient at extracting signals from background noise. This study demonstrates that spotted seals possess sound reception capabilities different from those previously described for ice seals, and more similar to those reported for harbor seals (Phoca vitulina). The results are consistent with the amphibious lifestyle of these seals and their apparent reliance on sound. The hearing data reported herein are the first available for spotted seals and can inform best management practices for this vulnerable species in a changing Arctic.

  4. Amphibious hearing in ringed seals (Pusa hispida): underwater audiograms, aerial audiograms and critical ratio measurements.

    Science.gov (United States)

    Sills, Jillian M; Southall, Brandon L; Reichmuth, Colleen

    2015-07-01

    Ringed seals (Pusa hispida) are semi-aquatic marine mammals with a circumpolar Arctic distribution. In this study, we investigate the amphibious hearing capabilities of ringed seals to provide auditory profiles for this species across the full range of hearing. Using psychophysical methods with two trained ringed seals, detection thresholds for narrowband signals were measured under quiet, carefully controlled environmental conditions to generate aerial and underwater audiograms. Masked underwater thresholds were measured in the presence of octave-band noise to determine critical ratios. Results indicate that ringed seals possess hearing abilities comparable to those of spotted seals (Phoca largha) and harbor seals (Phoca vitulina), and considerably better than previously reported for ringed and harp seals. Best sensitivity was 49 dB re. 1 µPa (12.8 kHz) in water, and -12 dB re. 20 µPa (4.5 kHz) in air, rivaling the acute hearing abilities of some fully aquatic and terrestrial species in their respective media. Critical ratio measurements ranged from 14 dB at 0.1 kHz to 31 dB at 25.6 kHz, suggesting that ringed seals--like other true seals--can efficiently extract signals from background noise across a broad range of frequencies. The work described herein extends similar research on amphibious hearing in spotted seals recently published by the authors. These parallel studies enhance our knowledge of the auditory capabilities of ice-living seals, and inform effective management strategies for these and related species in a rapidly changing Arctic environment. © 2015. Published by The Company of Biologists Ltd.

  5. Transmission of UV-irradiance into nectarine fruit

    International Nuclear Information System (INIS)

    Blanke, M.M.

    1996-01-01

    With the global depletion of the ozone layer, leaves and fruits are increasingly exposed to UV-irradiance on the tree. Some fruits are additionally exposed postharvest to artificial germicidal W-irradiance, leading to a cumulative effect. This paper examines the transmission of UV-light (200-400 nm) by the peel of ripe nectarine fruit using UV/VIS spectrophotometry to aid understanding of UV-effects and assess the sensitivity of the peel to UV wavelengths. Yellow peel of nectarine fruit transmitted less than 0.1 % in the UV-C range of 220 to 280 nm. With longer wavelenghts, UV-light transmission increased slowly from 0.4 % at 284 nm to 1.6 % at 320 nm and, in the UV-A region, progressively from 1.9 % at 330 nm to a maximum of 13 % of incident irradiance at 400 nm. Red peel of nectarine fruit transmitted less than 0.1 % of UV-C and UV-B light, but up to 0.9 % of incident UV-A light at 400 nm. Conversely, UV-absorption of nectarine peel decreased with longer wavelengths. Hence, fruit parenchyma is more affected by UV-irradiance at wavelengths above ca. 280 nm and underneath yellow than underneath red peel

  6. Exposure of harbour seals Phoca vitulina to Brucella in declining populations across Scotland.

    Science.gov (United States)

    Kershaw, Joanna L; Stubberfield, Emma J; Foster, Geoffrey; Brownlow, Andrew; Hall, Ailsa J; Perrett, Lorraine L

    2017-09-20

    Since 2000 there has been a major decline in the abundance of Scottish harbour seals Phoca vitulina. The causes of the decline remain uncertain. The aim of this study was to establish the extent to which the seals in the regions of greatest decline have been exposed to Brucella, a bacterial pathogen that causes reproductive failure in terrestrial mammalian hosts. Tissues from dead seals collected between 1992 and 2013 were cultured for Brucella (n = 150). Serum samples collected from live capture-released seals (n = 343) between 1997 and 2012 were tested for Brucella antibodies using the Rose Bengal plate agglutination test (RBT) and a competitive enzyme-linked immunosorbent assay (cELISA). In total, 16% of seals cultured had Brucella isolated from one or more tissues, but there were no pathological signs of infection. The cELISA results were more sensitive than the RBT results, showing that overall 25.4% of seals were seropositive, with the highest seroprevalence in juveniles. As there was no evidence of either a higher seroprevalence or higher circulating antibody levels in seropositive animals in the areas with the greatest declines, it was concluded that Brucella infection is likely not a major contributing factor to recent declines. However, the consistently high proportion of seals exposed to Brucella indicates possible endemicity in these populations, likely due to B. pinnipedialis, which has demonstrated a preference for pinniped hosts. Importantly, given the close proximity between seals, humans and livestock in many areas, there is the potential for cross-species infections.

  7. Turbine Seal Research at NASA GRC

    Science.gov (United States)

    Proctor, Margaret P.; Steinetz, Bruce M.; Delgado, Irebert R.; Hendricks, Robert C.

    2011-01-01

    Low-leakage, long-life turbomachinery seals are important to both Space and Aeronautics Missions. (1) Increased payload capability (2) Decreased specific fuel consumption and emissions (3) Decreased direct operating costs. NASA GRC has a history of significant accomplishments and collaboration with industry and academia in seals research. NASA's unique, state-of-the-art High Temperature, High Speed Turbine Seal Test Facility is an asset to the U.S. Engine / Seal Community. Current focus is on developing experimentally validated compliant, non-contacting, high temperature seal designs, analysis, and design methodologies to enable commercialization.

  8. Effect of UV irradiation on the apoptosis and necrosis of Jurkat cells using UV LEDs

    Science.gov (United States)

    Inada, Shunko A.; Amano, Hiroshi; Akasaki, Isamu; Morita, Akimichi; Kobayashi, Keiko

    2009-02-01

    Phototherapy is a very effective method for treating most of the incurable skin diseases. A fluorescent light bulb is used as a conventional UV light source for this type of therapy. However, infrared radiation from the light source sometimes causes serious problems on patient's health. In addition, the normal part of the skin is irradiated when a large fluorescent light bulb is used. Moreover, a conventional UV irradiation system is heavy and has a short lifetime and a high electrical power consumption. Therefore, a new UV light source for solving the problems of phototherapy is required. To realize low-power-consumption, lightweight and long-lifetime systems, group III nitride-based UV-A1 light-emitting diodes (LEDs) were investigated. We examined the UV LED irradiation of Jurkat cell, which is a tumor cell and more sensitive to UV light than a healthy cell. The numbers of apoptotic and necrotic cells were confirmed to be the same using a UV LED and a conventional lamp system. The UV LED showed the possibility of realizing a new UV light source for phototherapy.

  9. Double angle seal forming lubricant film

    Science.gov (United States)

    Ernst, William D.

    1984-01-01

    A lubricated piston rod seal which inhibits gas leaking from a high pressure chamber on one side of the seal to a low pressure chamber on the other side of the seal. A liquid is supplied to the surface of the piston rod on the low pressure side of the seal. This liquid acts as lubricant for the seal and provides cooling for the rod. The seal, which can be a plastic, elastomer or other material with low elastic modulus, is designed to positively pump lubricant through the piston rod/seal interface in both directions when the piston rod is reciprocating. The capacity of the seal to pump lubricant from the low pressure side to the high pressure side is less than its capacity to pump lubricant from the high pressure side to the low pressure side which ensures that there is zero net flow of lubricant to the high pressure side of the seal. The film of lubricant between the seal and the rod minimizes any sliding contact and prevents the leakage of gas. Under static conditions gas leakage is prevented by direct contact between the seal and the rod.

  10. Air riding seal with purge cavity

    Science.gov (United States)

    Sexton, Thomas D; Mills, Jacob A

    2017-08-15

    An air riding seal for a turbine in a gas turbine engine, where an annular piston is axial moveable within an annular piston chamber formed in a stator of the turbine and forms a seal with a surface on the rotor using pressurized air that forms a cushion in a pocket of the annular piston. A purge cavity is formed on the annular piston and is connected to a purge hole that extends through the annular piston to a lower pressure region around the annular piston or through the rotor to an opposite side. The annular piston is sealed also with inner and outer seals that can be a labyrinth seal to form an additional seal than the cushion of air in the pocket to prevent the face of the air riding seal from overheating.

  11. Insecurity of imperfect quantum bit seal

    International Nuclear Information System (INIS)

    Chau, H.F.

    2006-01-01

    Quantum bit seal is a way to encode a classical bit quantum mechanically so that everyone can obtain non-zero information on the value of the bit. Moreover, such an attempt should have a high chance of being detected by an authorized verifier. Surely, a reader looks for a way to get the maximum amount of information on the sealed bit and at the same time to minimize her chance of being caught. And a verifier picks a sealing scheme that maximizes his chance of detecting any measurement of the sealed bit. Here, I report a strategy that passes all measurement detection procedures at least half of the time for all quantum bit sealing schemes. This strategy also minimizes a reader's chance of being caught under a certain scheme. In this way, I extend the result of Bechmann-Pasquinucci et al. by proving that quantum seal is insecure in the case of imperfect sealed bit recovery

  12. Development of the seal for nuclear material

    International Nuclear Information System (INIS)

    Lu Feng; Lu Zhao; Zhao Yonggang; Zhang Qixin; Xiao Xuefu

    2000-01-01

    Two kinds of double cap metallic seal and an adhesive seal are developed for the purpose of the accounting for and control of nuclear material. Two kinds of double cap metallic seal are made of stainless steel and copper, respectively and the self-locked technique is used. The number and the random pattern are carved out side and in side of a cap, respectively, for the seal. The random pattern carved inside of a cap for seal is taken a picture using numeral camera and memorized in computer. Special software is developed for verification of the random pattern memorized in computer. The adhesive seal is made of special adhesive paper for purpose of security, and a special pattern guarded against falsification is printed on seal paper using ultraviolet fluorescent light technique

  13. Comets in UV

    Science.gov (United States)

    Shustov, B.; Sachkov, M.; Gómez de Castro, A. I.; Vallejo, J. C.; Kanev, E.; Dorofeeva, V.

    2018-04-01

    Comets are important "eyewitnesses" of Solar System formation and evolution. Important tests to determine the chemical composition and to study the physical processes in cometary nuclei and coma need data in the UV range of the electromagnetic spectrum. Comprehensive and complete studies require additional ground-based observations and in situ experiments. We briefly review observations of comets in the ultraviolet (UV) and discuss the prospects of UV observations of comets and exocomets with space-borne instruments. A special reference is made to the World Space Observatory-Ultraviolet (WSO-UV) project.

  14. Core disruptive accident margin seal

    International Nuclear Information System (INIS)

    Garin, J.; Belsick, J.C.

    1978-01-01

    Disclosed is an apparatus for sealing the annulus defined between a substantially cylindrical rotatable first riser assembly and plug combination disposed in a substantially cylindrical second riser assembly and plug combination of a nuclear reactor system. The apparatus comprises a flexible member disposed between the first and second riser components and attached to a metal member which is attached to an actuating mechanism. When the actuating mechanism is not actuated, the flexible member does not contact the riser components thus allowing the free rotation of the riser components. When desired, the actuating mechanism causes the flexible member to contact the first and second riser components in a manner to block the annulus defined between the riser components, thereby sealing the annulus between the riser components

  15. Transport of multiassembly sealed canisters

    International Nuclear Information System (INIS)

    Quinn, R.D.; Lehnert, R.A.; Rosa, J.M.

    1992-01-01

    A significant portion of the commercial spent nuclear fuel in dry storage in the US will be stored in multiassembly sealed canisters before the DOE begins accepting fuel from utilities in 1998. This paper reports that it is desirable from economic and ALARA perspectives to transfer these canisters directly from the plant to the MRS. To this end, it is necessary that the multiassembly sealed canisters, which have been licensed for storage under 10CFR72, be qualified for shipment within a suitable shipping cask under the rules of 10CFR71. Preliminary work performed to date indicates that it is feasible to license a current canister design for transportation, and work is proceeding on obtaining NRC approval

  16. Hermetically sealed superconducting magnet motor

    Science.gov (United States)

    DeVault, Robert C.; McConnell, Benjamin W.; Phillips, Benjamin A.

    1996-01-01

    A hermetically sealed superconducting magnet motor includes a rotor separated from a stator by either a radial gap, an axial gap, or a combined axial and radial gap. Dual conically shaped stators are used in one embodiment to levitate a disc-shaped rotor made of superconducting material within a conduit for moving cryogenic fluid. As the rotor is caused to rotate when the field stator is energized, the fluid is pumped through the conduit.

  17. Fabrication of sealed radiation sources

    International Nuclear Information System (INIS)

    Mars, Jean.

    1977-01-01

    The description is given for fabricating a sealed radiation source, consisting in depositing on a metal substrate a thin active coat of a radioelement, termed first coat, submitting this coated substrate to an oxidation treatment in order to obtain on the first coat an inactive coat of an oxide of the metal, termed second coat, and depositing a coat of varnish on this second inactive coat [fr

  18. Seals for sealing a pressure vessel such as a nuclear reactor vessel or the like

    International Nuclear Information System (INIS)

    Bruns, H.J.; Huelsermann, K.H.

    1975-01-01

    A description is given of seals for sealing a pressure vessel such as a nuclear reactor vessel, steam boiler vessel, or any other vessel which is desirably sealed against pressure of the type including a housing and a housing closure that present opposed vertical sealing surfaces which define the sides of a channel. The seals of the present invention comprise at least one sealing member disposed in the channel, having at least one stop face, a base portion and two shank portions extending from the base portion to form a groove-like recess. The shank portions are provided with sealing surfaces arranged to mate with the opposed vertical pressure vessel sealing surfaces. A shank-spreading wedge element also disposed in the channel has at least one stop face and is engaged in the groove-like recess with the sealing member and wedge element stop face adjacent to each other

  19. UV exposure in cars.

    Science.gov (United States)

    Moehrle, Matthias; Soballa, Martin; Korn, Manfred

    2003-08-01

    There is increasing knowledge about the hazards of solar and ultraviolet (UV) radiation to humans. Although people spend a significant time in cars, data on UV exposure during traveling are lacking. The aim of this study was to obtain basic information on personal UV exposure in cars. UV transmission of car glass samples, windscreen, side and back windows and sunroof, was determined. UV exposure of passengers was evaluated in seven German middle-class cars, fitted with three different types of car windows. UV doses were measured with open or closed windows/sunroof of Mercedes-Benz E 220 T, E 320, and S 500, and in an open convertible car (Mercedes-Benz CLK). Bacillus subtilis spore film dosimeters (Viospor) were attached to the front, vertex, cheeks, upper arms, forearms and thighs of 'adult' and 'child' dummies. UV wavelengths longer than >335 nm were transmitted through car windows, and UV irradiation >380 nm was transmitted through compound glass windscreens. There was some variation in the spectral transmission of side windows according to the type of glass. On the arms, UV exposure was 3-4% of ambient radiation when the car windows were shut, and 25-31% of ambient radiation when the windows were open. In the open convertible car, the relative personal doses reached 62% of ambient radiation. The car glass types examined offer substantial protection against short-wave UV radiation. Professional drivers should keep car windows closed on sunny days to reduce occupational UV exposure. In individuals with polymorphic light eruption, produced by long-wave UVA, additional protection by plastic films, clothes or sunscreens appears necessary.

  20. Steam Turbine Flow Path Seals (a Review)

    Science.gov (United States)

    Neuimin, V. M.

    2018-03-01

    Various types of shroud, diaphragm, and end seals preventing idle leak of working steam are installed in the flow paths of steam turbine cylinders for improving their efficiency. Widely known labyrinth seals are most extensively used in the Russian turbine construction industry. The category of labyrinth seals also includes seals with honeycomb inserts. The developers of seals with honeycomb inserts state that the use of such seals makes it possible to achieve certain gain due to smaller leaks of working fluid and more reliable operation of the system under the conditions in which the rotor rotating parts may rub against the stator elements. However, a positive effect can only be achieved if the optimal design parameters of the honeycomb structure are fulfilled with due regard to the specific features of its manufacturing technology and provided that this structure is applied in a goal-seeking manner in the seals of steam and gas turbines and compressors without degrading their vibration stability. Calculated and preliminary assessments made by experts testify that the replacement of conventional labyrinth seals by seals with honeycomb inserts alone, due to which the radial gaps in the shroud seal can be decreased from 1.5 to 0.5 mm, allows the turbine cylinder efficiency to be increased at the initial stage by approximately 1% with the corresponding gain in the turbine set power output. The use of rectangular-cellular seals may result, according to estimates made by their developers, in a further improvement of turbine efficiency by 0.5-1.0%. The labor input required to fabricate such seals is six to eight times smaller than that to fabricate labyrinth seals with honeycomb inserts. Recent years have seen the turbine construction companies of the United States and Germany advertising the use of abradable (sealing) coatings (borrowed from the gas turbine construction technology) in the turbine designs instead of labyrinth seals. The most efficient performance of

  1. UV-induced effects

    NARCIS (Netherlands)

    Liebsch, M.; Spielmann, H.; Pape, W.; Krul, C.; Deguercy, A.; Eskes, C.A.M.

    2005-01-01

    Regulatory requirements: According to the current Notes for Guidance of the Scientific Committee on Cosmetic Products and Non-Food Products (SCCNFP), cosmetic ingredients and mixtures of ingredients absorbing UV light (in particular UV filter chemicals used, for example, to ensure the light

  2. Molecular Engineering of UV/Vis Light-Emitting Diode (LED)-Sensitive Donor-π-Acceptor-Type Sulfonium Salt Photoacid Generators: Design, Synthesis, and Study of Photochemical and Photophysical Properties.

    Science.gov (United States)

    Wu, Xingyu; Jin, Ming; Xie, Jianchao; Malval, Jean-Pierre; Wan, Decheng

    2017-11-07

    A series of donor-π-acceptor-type sulfonium salt photoacid generators (PAGs) were designed and synthesized by systematically changing electron-donating groups, π-conjugated systems, electron-withdrawing groups, and the number of branches through molecular engineering. These PAGs can effectively decompose under UV/Vis irradiation from a light-emitting diode (LED) light source because of the matching absorption and emitting spectra of the LEDs. The absorption and acid-generation properties of these sulfonium salts were elucidated by UV/Vis spectroscopy and so forth. Results indicated that the PAG performance benefited from the introduction of strong electron-donating groups, specific π-conjugated structures, certain electron-withdrawing groups, or two-branched structures. Most sulfonium salts showed potential as photoinitiators under irradiation by a wide variety of UV and visible LEDs. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. Comparison of Sensitivity to Photoinhibition and UV-B Stress between Developing and Mature Leaves of Red Pepper (Capsicum annuum L.) Plants from Control and Gamma-Irradiated Seeds

    International Nuclear Information System (INIS)

    Kim, J.H.; Baek, M.H.; Chung, B.Y.; Kim, J.S.; Lee, Y.B.

    2004-01-01

    The stress-resistance of red pepper (Capsicum annuum L. cv. Yeomyung and Joheung) plants from the seeds irradiated with low doses of gamma-radiation (2, 4, 8, and 16 Gy) was examined under the conditions of photoinhibition and UV-B stress. To induce photoinhibition, the leaves acclimated overnight with a dim light were exposed to a photon flux density (PFD) of 200 μmol · m-² · s-¹ at 25℃ for 1 h. Then, they were further exposed to the same PFD with supplementary UV-B irradiance at 25℃ for 3 h (UV-B stress). The gamma-irradiation stimulated the early growth of the plants in both cultivars at 2, 4, and 8 Gy

  4. Design optimization of seal structure for sealing liquid by magnetic fluids

    International Nuclear Information System (INIS)

    Liu Tonggang; Cheng Yusheng; Yang Zhiyi

    2005-01-01

    The durability of the magnetic fluid seal clearly decreases when sealing another liquid because of the interface instability caused by the applied magnetic field and the velocity difference of the two liquids. With an intention to establish a stable interface during sealing liquid, a new magnetic fluid seal was developed in this paper. The parameters of the structure were optimized by a simulation apparatus. And the magnetic fluid seal designed based on the optimum parameters shows good performance and long life for sealing lubricating oil

  5. Vacuum sealing with a spiral grooved gas dynamic seal

    International Nuclear Information System (INIS)

    Sawada, Tadashi

    1979-01-01

    Gas dynamic seals with rectangular spiral grooves are studied theoretically taking the effects of sidewalls of the grooves and the effects of gas compressibility into account, and slip boundary conditions are employed. The results are compared with the existing experimental data and the validity of the theory is confirmed over a wide pressure range except for the extremely low pressures. Suggestions are made regarding the choice of the geometrical dimensions, i.e., aspect ratio, helix angle, clearance parameter and groove width ratio. (author)

  6. Mechanisms of uv mutagenesis in yeast

    International Nuclear Information System (INIS)

    Lawrence, C.W.; Christensen, R.; Schwartz, A.

    1982-01-01

    The uv mutagenesis in yeast depends on the function of the RAD6 locus, a gene that is also responsible for a substantial fraction of wild-type resistance, suggesting that this eukaryote may possess a misrepair mechanism analogous to that proposed for Escherichia coli. The molecular mechanism responsible for RAD6 repair or recovery is not yet known, but it is different from either excision or recombination-dependent repair, processes carried out by the other two main repair pathways in yeast. RAD6-dependent mutagenesis has been found to have the following characteristics. It is associated at best with only a small fraction of RAD6-dependent repair, the majority of the sensitivity of rad6 mutants being due to their lack of nonmutagenic repair. SRS2 metabolic suppressors restore a substantial fraction of uv resistance to rad6 mutants but do not restore their uv mutability. Strains containing mutations at loci (rev, umr) that are probably more directly involved in mutagenesis are only mildly sensitive, and there is a poor correlation between their sensitivity and mutational deficiency. The uv mutagenesis appears to require a large number of gene functions, perhaps ten or more. Where examined in detail, these genes have been found to be concerned in the production of only a specific range of mutational events, not all of them. Mating experiments have shown that a substantial fraction, probably 40% or more, of uv-induced mutations are untargeted, that is, occur in lesion-free regions of DNA. The uv irradiation, therefore, produces a general reduction in the normally high fidelity with which DNA is replicated on undamaged templates. It does not appear to be necessary for the causal lesion to be present in the same chromosome as the mutation it induces. The reduction in fidelity may be the consequence of the production of a diffusible factor in uv-irradiated cells, but definite evidence supporting this proposal has not yet been obtained

  7. Actively controlled shaft seals for aerospace applications

    Science.gov (United States)

    Salant, Richard F.

    1995-07-01

    This study experimentally investigates an actively controlled mechanical seal for aerospace applications. The seal of interest is a gas seal, which is considerably more compact than previous actively controlled mechanical seals that were developed for industrial use. In a mechanical seal, the radial convergence of the seal interface has a primary effect on the film thickness. Active control of the film thickness is established by controlling the radial convergence of the seal interface with a piezoelectric actuator. An actively controlled mechanical seal was initially designed and evaluated using a mathematical model. Based on these results, a seal was fabricated and tested under laboratory conditions. The seal was tested with both helium and air, at rotational speeds up to 3770 rad/sec, and at sealed pressures as high as 1.48 x 10(exp 6) Pa. The seal was operated with both manual control and with a closed-loop control system that used either the leakage rate or face temperature as the feedback. The output of the controller was the voltage applied to the piezoelectric actuator. The seal operated successfully for both short term tests (less than one hour) and for longer term tests (four hours) with a closed-loop control system. The leakage rates were typically 5-15 slm (standard liters per minute), and the face temperatures were generally maintained below 100C. When leakage rate was used as the feedback signal, the setpoint leakage rate was typically maintained within 1 slm. However, larger deviations occurred during sudden changes in sealed pressure. When face temperature was used as the feedback signal, the setpoint face temperature was generally maintained within 3 C, with larger deviations occurring when the sealed pressure changes suddenly. the experimental results were compared to the predictions from the mathematical model. The model was successful in predicting the trends in leakage rate that occurred as the balance ratio and sealed pressure changed

  8. Quantification of biologically effective environmental UV irradiance

    Science.gov (United States)

    Horneck, G.

    To determine the impact of environmental UV radiation on human health and ecosystems demands monitoring systems that weight the spectral irradiance according to the biological responses under consideration. In general, there are three different approaches to quantify a biologically effective solar irradiance: (i) weighted spectroradiometry where the biologically weighted radiometric quantities are derived from spectral data by multiplication with an action spectrum of a relevant photobiological reaction, e.g. erythema, DNA damage, skin cancer, reduced productivity of terrestrial plants and aquatic foodweb; (ii) wavelength integrating chemical-based or physical dosimetric systems with spectral sensitivities similar to a biological response curve; and (iii) biological dosimeters that directly weight the incident UV components of sunlight in relation to the effectiveness of the different wavelengths and to interactions between them. Most biological dosimeters, such as bacteria, bacteriophages, or biomolecules, are based on the UV sensitivity of DNA. If precisely characterized, biological dosimeters are applicable as field and personal dosimeters.

  9. Guide to optimized replacement of equipment seals

    International Nuclear Information System (INIS)

    Gleason, J.F.

    1990-03-01

    A reevaluation of current scheduled replacement intervals of polymeric seals in plant equipment can achieve significant benefits. Information is provided which has the potential for increasing replacement intervals based on better information on how seals have performed through unique nuclear industry tests to qualify equipment, improved elastomers and increased knowledge of the failure mechanisms and related performance. The research was performed by reviewing applications of elastomeric seals in nuclear plants and practice associated with defining seal replacement intervals in the nuclear power and other industries. Performance indicators and how they predict degradation of seals were evaluated. Guidelines and a flow chart for reevaluating seal replacement intervals are provided. 29 refs., 38 figs., 8 tabs

  10. Seal analysis technology for reactor pressure vessel

    International Nuclear Information System (INIS)

    Zheng Liangang; Zhang Liping; Yang Yu; Zang Fenggang

    2009-01-01

    There is the coolant with radiation, high temperature and high pressure in the reactor pressure vessel (RPV). It is closely correlated to RPV sealing capability whether the whole nuclear system work well or not. The aim of this paper is to study the seal analysis method and technology, such as the pre-tensioning of the bolt, elastoplastic contact and coupled technology of thermal and structure. The 3 D elastoplastic seal analysis method really and generally consider the loads and model the contact problem with friction between the contact plates. This method is easier than the specialized seal program and used widely. And it is more really than the 2 D seal analysis method. This 3 D elastoplastic seal analysis method has been successfully used in the design and analysis of RPV. (authors)

  11. Folded membrane dialyzer with mechanically sealed edges

    Energy Technology Data Exchange (ETDEWEB)

    Markley, F.W.

    A semipermeable membrane is folded in accordion fashion to form a stack of pleats and the edges are sealed so as to isolate the opposite surfaces of the membrane. The stack is contained within a case that provides ports for flow of blood in contact with one surface of the membrane through channels formed by the pleats and also provides ports for flow of a dialysate through channels formed by the pleats in contact with the other surface of the membrane. The serpentine side edges of the membrane are sealed by a solidified plastic material, whereas effective mechanical means are provided to seal the end edges of the folded membrane. The mechanical means include a clamping strip which biases case sealing flanges into a sealed relationship with end portions of the membrane near the end edges, which portions extend from the stack and between the sealing flanges.

  12. Executive summary and general conclusions of the rock sealing project

    International Nuclear Information System (INIS)

    Pusch, R.

    1992-06-01

    The Stripa Rock Sealing Project logically followed the two first Stripa research phases dealing with canister-embedment and plugging of excavations in repositories. The major activities in the third phase were: * Literature review and interviews for setting the state of art of rock fracture sealing. * Pilot field and lab testing applying a new effective 'dynamic' grouting technique. * Development of a general grout flow theory. * Investigation of physical properties and longevity of major candidate grouts. * Performance of 4 large-scale tests. The literature study showed that longevity aspects limited the number of potentially useful grout materials to smectitic clay and cement. The pilot testing showed that fine-grained grouts can be effectively injected in relatively fine fractures. The theoretical work led to a general grout flow theory valid both for grouting at a constant, static pressure with non-Newtonian material properties, and for 'dynamic' injection with superimposed oscillations, yielding Newtonian material behavior. The investigation of physical properties of candidate grouts with respect to hydraulic conductivity, shear strength, sensitivity to mechanical strain, as well as to chemical stability, showed that effective sealing is offered, and that any rock can have its bulk conductivity reduced to about 10 -10 m/s. The field tests comprised investigation of excavation-induced disturbance and attempts to seal disturbed rock, and in separate tests, grouting of deposition holes and a natural fine-fracture zone. Considerable disturbance of nearfield rock by blasting and stress changes, yielding an increase in axial hydraulic conductivity by 3 and 1 order of magnitude, respectively, was documented but various factors, primarily debris in the fractures, made grouting of blasted rock ineffective. Narrow fractures in deposition holes and in a natural fracture zone were sealed rather effectively. (au)

  13. Management of disused sealed sources

    International Nuclear Information System (INIS)

    Lukauskas, D.; Skridaila, N.

    2003-01-01

    The report presents the requirements on management of disused sealed sources in Lithuania; disused sealed source disposal facilities; performed safety analysis and planed repository safety improvements. The requirements on pre-disposal management of Disused Sealed Sources (DSS) are presented. The requirements on disposal of short lived VLLW and LILW (A, B and C classes) radioactive waste approved in 2002-2003. Generic Waste Acceptance Criteria for Near Surface Disposal, P-2003-01 approved in 2003. Requirements on disposal of Low and intermediate level long lived waste do not exist (D and E classes). Requirements for the disposal of disused sealed sources (F class) do not exist. Disposal method for the F class - Near Surface or Deep geological repository, depending on the waste acceptance criteria. Only one repository for institutional radioactive waste exist in Lithuania - Maisiagala repository. It is near surface RADON type disposal facility, built in 1963 and closed in 1988. It was constructed of the monolithic reinforced concrete with the dimensions 5 m x 15 m x 3 m, the thickness of the sidewalls is about 0.25 m and the thickness of the bottom is about 0.2 m. The overall volume is about 200 m 3 . At time of closure only three fifths of the volume had been filled. The empty two fifths of the vault were filled with concrete, then with sand, then with the concrete (0.01 m), hot bitumen and the 0.05 m asphalt layers. Monolithic concrete that was covered with bitumen and 0.05 m thick layer of asphalt closed the vault. Sand layer the thickness of which was not less than 1.2 m formed the cap. Disused radioactive sources embedded in a biological shielding were buried together with their shielding, the sources without the shielding were buried in two stainless steel containers. The total activity of buried radioactive nuclides is 3.42.10 -15 Bq (calculated according the documentation). There are some uncertainties about the inventory: from 1963 to 1973. After the

  14. Sealing method and sealing device for radioactive waste containing vessel

    International Nuclear Information System (INIS)

    Ishiwatari, Koji; Otsuki, Akira

    1998-01-01

    A radioactive waste-containing body is hoisted down into a strong-material vessel opened upwardly, and a strong-material lid is hoisted down to the opening of the strong-material-vessel and welded. The strong material vessel is hoisted up and loaded on a corrosion resistant-material bottom plate placed horizontally. A corrosion resistant-material vessel having one opening end and having a corrosion resistant-material flange on the other end and previously agreed with the strong material-vessel main body is hoisted up by a hoisting device having an inserting device so that the opening of the corrosion resistant vessel is directed downwardly. The corrosion resistant vessel is press-fitted to the outside of the strong material-vessel by the inserting device while being heated by a preheater to shrink. Subsequently, the lower end of the corrosion resistant-material vessel and the corrosion resistant-material bottom plate are welded to constitute a corrosion resistant-material vessel. Then, the radioactive waste containing body can be sealed in a sealing vessel comprising the strong-material vessel and the corrosion resistant-material vessel. (N.H.)

  15. Comment on 'Quantum string seal is insecure'

    International Nuclear Information System (INIS)

    He Guangping

    2007-01-01

    An attack strategy was recently proposed by Chau [Phys. Rev. A 75, 012327 (2007)], which was claimed to be able to break all quantum string seal protocols. Here it will be shown that the attack cannot obtain nontrivial information and escape the detection simultaneously in a class of quantum string seal, including the one proposed by He [Int. J. Quantum Inf. 4, 677 (2006)]. Thus it is insufficient to conclude that all quantum string seals are insecure

  16. A seal analyzer for testing container integrity

    International Nuclear Information System (INIS)

    McDaniel, P.; Jenkins, C.

    1988-01-01

    This paper reports on the development of laboratory and production seal analyzer that offers a rapid, nondestructive method of assuring the seal integrity of virtually any type of single or double sealed container. The system can test a broad range of metal cans, drums and trays, membrane-lidded vessels, flexible pouches, aerosol containers, and glass or metal containers with twist-top lids that are used in the chemical/pesticide (hazardous materials/waste), beverage, food, medical and pharmaceutical industries

  17. Ceramic blade with tip seal

    Science.gov (United States)

    Glezer, B.; Bhardwaj, N.K.; Jones, R.B.

    1997-08-05

    The present gas turbine engine includes a disc assembly defining a disc having a plurality of blades attached thereto. The disc has a preestablished rate of thermal expansion and the plurality of blades have a preestablished rate of thermal expansion being less than the preestablished rate of thermal expansion of the disc. A shroud assembly is attached to the gas turbine engine and is spaced from the plurality of blades a preestablished distance forming an interface there between. Positioned in the interface is a seal having a preestablished rate of thermal expansion being generally equal to the rate of thermal expansion of the plurality of blades. 4 figs.

  18. The Sealed Tube Neutron Generator

    International Nuclear Information System (INIS)

    Tunnell, L.N.; Beyerle, A.; Durkee, R.; Headley, G.; Hurley, P.

    1992-01-01

    A Sealed Tube Neutron Generator (STNG) has been designed and tested at Special Technologies Laboratories (STL) in Santa Barbara, California. Unlike similar tubes that have been used for years in other applications, e.g., by the oil well logging industry, the present device was designed primarily to be part of the Associated Particle Imaging (API) system. Consequently, the size and quality of the neutron spot produced by the STNG is of primary importance. Results from initial measurements indicate that performance goals are satisfied

  19. Upgrading primary heat transport pump seals

    International Nuclear Information System (INIS)

    Graham, T.; Metcalfe, R.; Rhodes, D.; McInnes, D.

    1995-01-01

    Changes in the operating environment at the Bruce-A Nuclear Generating Station created the need for an upgraded Primary Heat Transport Pump (PHTP) seal. In particular, the requirement for low pressure running during more frequent start-ups exposed a weakness of the CAN2 seal and reduced its reliability. The primary concern at Bruce-A was the rotation of the CAN2 No. 2 stators in their holders. The introduction of low pressure running exacerbated this problem, giving rapid wear of the stator back face, overheating, and thermocracking. In addition, the resulting increase in friction between the stator and its holder increased stationary-side hysteresis and thereby changed the seal characteristic to the point where interseal pressure oscillations became prevalent. The resultant increased hysteresis also led to hard rubbing of the seal faces during temperature transients. An upgraded seal was required for improved reliability to avoid forced outages and to reduce maintenance costs. This paper describes this upgraded 'replacement seal' and its performance history. In spite of the 'teething' problems detailed in this paper, there have been no forced outages due to the replacement seal, and in the words of a seal maintenance worker at Bruce-A, 'it allows me to go home and sleep at night instead of worrying about seal failures.' (author)

  20. Seal for an object containing nuclear fuel

    International Nuclear Information System (INIS)

    Scheuerpflug, W.; Nentwich, D.

    1977-01-01

    This seal which cannot be counterfeited, specially for sealing nuclear objects, e.g. fuel rods, not only makes any damage which has taken place obvious, but makes identification according to a key possible. For this purpose a minimum number of 'particles' or small bodies, which are identical but of different permeability, are fixed inside a short tube during 'loading' of the seal in a certain or an accidental sequence. The sequence of the spheres, which represents a key, can only be determined by special electromagnetic measuring equipment. On first opening the seal, this key sequence is irrevocably destroyed. (HP) [de

  1. Quick-sealing design for radiological containment

    International Nuclear Information System (INIS)

    Rampdla, D.S.; Speer, E.

    1991-01-01

    This patent describes a quick-sealing assembly and method for forming an adhesive seal on opposite sides of a mechanical seal for a flexible containment bag of the type used for working with radioactively contaminated objects. The assembly includes an elongated mechanical fastener having opposing engaging members affixed at a predetermined distance from each of the elongated edges, with an adhesive layer formed between the mechanical fastener and the elongated edge such that upon engagement of the mechanical fastener and adhesive layers to opposing containment fabric, a neat triple hermetic seal is formed

  2. Quick-sealing design for radiological containment

    International Nuclear Information System (INIS)

    Rampolia, D.S.; Speer, E.

    1990-01-01

    This patent describes a quick-sealing assembly and method for forming an adhesive seal on opposite sides of a mechanical seal for a flexible containment bag of the type used for working with radioactively contaminated objects. The assembly includes an elongated mechanical fastener having opposing engaging members affixed at a predetermined distance from each of the elongated edges, with an adhesive layer formed between the mechanical fastener and the elongated edge such that upon engagement of the mechanical fastener and adhesive layers to opposing containment fabric, a neat triple hermetic seal is formed

  3. Reusable tamper-indicating security seal

    International Nuclear Information System (INIS)

    Ryan, M.J.

    1981-01-01

    A reusable tamper-indicating mechanical security seal for use in safeguarding nuclear material has been developed. The high-security seal displays an unpredictable, randomly selected, five-digit code each time it is used. This five digit code serves the same purpose that the serial number does for conventional non-reusable seals - a unique identifier for each use or application. The newly developed reusable seal is completely enclosed within a seamless, tamper-indicating, plastic jacket. The jacket is designed to reveal any attempts to penetrate, section or to chemically remove and replace with a counterfeit for surreptitious purposes

  4. Ductile alloys for sealing modular component interfaces

    Science.gov (United States)

    Marra, John J.; Wessell, Brian J.; James, Allister W.; Marsh, Jan H.; Gear, Paul J.

    2017-08-08

    A vane assembly (10) having: an airfoil (12) and a shroud (14) held together without metallurgical bonding there between; a channel (22) disposed circumferentially about the airfoil (12), between the airfoil (12) and the shroud (14); and a seal (20) disposed in the channel (22), wherein during operation of a turbine engine having the vane assembly (10) the seal (20) has a sufficient ductility such that a force generated on the seal (20) resulting from relative movement of the airfoil (12) and the shroud (14) is sufficient to plastically deform the seal (20).

  5. Quick-sealing design for radiological containment

    Science.gov (United States)

    Rampolia, Donald S.; Speer, Elmer

    1990-01-01

    A quick-sealing assembly and method for forming an adhesive seal on opposite sides of a mechanical seal for a flexible containment bag of the type used for working with radioactively contaminated objects. The assembly includes an elongated mechanical fastener having opposing engaging members affixed at a predetermined distance from each of the elongated edges, with an adhesive layer formed between the mechanical fastener and the elongated edge such that upon engagement of the mechanical fastener and adhesive layers to opposing containment fabric, a neat triple hermetic seal is formed.

  6. Properties of Sealing Materials in Groundwater Wells

    DEFF Research Database (Denmark)

    Köser, Claus

    pellets as sealing material in groundwater wells. The way and the pattern, in which bentonite pellets are deposited, have been shown to have an effect on the swelling pressure of the bentonite seal. During the transport phase of pellets from the terrain to a given sedimentation depth, a sorting process......) into densities for clay/water systems has been developed. This method has successfully been used to evaluate e.g., macroporosity, homogenization of the bentonite seal during the hydration of water, hydraulic conductivity and the creation of channels in the bentonite seals. Based on the results obtained...

  7. Bingham Sealing and Application in Vacuum Clamping

    Science.gov (United States)

    Yao, S. M.; Teo, Wee Kin; Geng, Zunmin; Turner, Sam; Ridgway, Keith

    2011-12-01

    Vacuum clamping is extensively used in shell machining. In this paper a Bingham Sealing (BS) is presented and formulized based on Bingham plastic performance. The sealing capability of BS is evaluated in various cases. A new Bingham plastic is developed and the yield stress is measured. The performances of "O"ring sealing and BS with the developed Bingham plastic are compared to the static experiment. In this experiment the same vacuum is achieved and the distortion of the blade with BS is better than that with "O" ring sealing.

  8. Bingham Sealing and Application in Vacuum Clamping

    International Nuclear Information System (INIS)

    Yao, S M; Teo, Wee Kin; Geng Zunmin; Turner, Sam; Ridgway, Keith

    2011-01-01

    Vacuum clamping is extensively used in shell machining. In this paper a Bingham Sealing (BS) is presented and formulized based on Bingham plastic performance. The sealing capability of BS is evaluated in various cases. A new Bingham plastic is developed and the yield stress is measured. The performances of Or ing sealing and BS with the developed Bingham plastic are compared to the static experiment. In this experiment the same vacuum is achieved and the distortion of the blade with BS is better than that with 'O' ring sealing.

  9. UV disinfection of water

    International Nuclear Information System (INIS)

    Skipperud, E.; Johansen; Myhrstad, J.A.

    1978-01-01

    UV radiation has been found to have advantages over chloration for the disinfection of water. New regulations for dietary conditions on Norwegian ships introduced in 1974 led to increased use of UV disinfection, and this has in the following years spread to waterworks. The present article is based on a study to determine possible limitation. The nature of the injuries to the microorganisms is first discussed, together with repair mechanisms. A table is given showing the energy required for 90 and 100 percent inactivation of a number of microorganisms. Some other factors affecting UV inactivation are briefly mentioned. (JIW)

  10. Ceramic/metal seals. [refractory materials for hermetic seals for lighium-metal sulfide batteries

    Science.gov (United States)

    Bredbenner, A. M.

    1977-01-01

    Design criteria are discussed for a hermetic seal capable of withstanding the 450 C operating temperature of a lithium-metal sulfide battery system. A mechanical seal consisting of two high strength alloy metal sleeves welded or brazed to a conductor assembly and pressed onto a ceramic is described. The conductor center passes through the ceramic but is not sealed to it. The seal is effected on the outside of the taper where the tubular part is pressed down over and makes contact.

  11. Hydrodynamic simulation of X-UV laser-produced plasmas

    International Nuclear Information System (INIS)

    Fajardo, M.; Zeitoun, P.; Gauthier, J.C.

    2004-01-01

    With the construction of novel X-UV sources, such as V-UV FEL's (free-electron lasers), X-UV laser-matter interaction will become available at ultra-high intensities. But even table-top facilities such as X-UV lasers or High Harmonic Generation, are starting to reach intensities high enough to produce dense plasmas. X-UV laser-matter interaction is studied by a 1-dimensional hydrodynamic Lagrangian code with radiative transfer for a range of interesting X-UV sources. Heating is found to be very different for Z=12-14 elements having L-edges around the X-UV laser wavelength. Possible absorption mechanisms were investigated in order to explain this behaviour, and interaction with cold dense matter proved to be dominant. Plasma sensitivity to X-UV laser parameters such as energy, pulse duration, and wavelength was also studied, covering ranges of existing X-UV lasers. We found that X-UV laser-produced plasmas could be studied using table-top lasers, paving the way for future V-UV-FEL high intensity experiments. (authors)

  12. 30 CFR 75.337 - Construction and repair of seals.

    Science.gov (United States)

    2010-07-01

    ... sealed areas. (b) Prior to sealing, the mine operator shall— (1) Remove insulated cables, batteries, and other potential electric ignition sources from the area to be sealed when constructing seals, unless it... after April 18, 2008, the seal at the lowest elevation shall have a corrosion-resistant, non-metallic...

  13. Albinistic common seals (Phoca vitulina) and melanistic grey seals (Halichoerus grypus) rehabilitated in the Netherlands

    NARCIS (Netherlands)

    Osinga, Nynke; 't Hart, Pieter; Vader, Pieter C. van Voorst

    2010-01-01

    The Seal Rehabilitation and Research Centre (SRRC) in Pieterburen, The Netherlands, rehabilitates seals from the waters of the Wadden Sea, North Sea and Southwest Delta area. Incidental observations of albinism and melanism in common and grey seals are known from countries surrounding the North Sea.

  14. High thermal expansion, sealing glass

    Science.gov (United States)

    Brow, R.K.; Kovacic, L.

    1993-11-16

    A glass composition is described for hermetically sealing to high thermal expansion materials such as aluminum alloys, stainless steels, copper, and copper/beryllium alloys, which includes between about 10 and about 25 mole percent Na[sub 2]O, between about 10 and about 25 mole percent K[sub 2]O, between about 5 and about 15 mole percent Al[sub 2]O[sub 3], between about 35 and about 50 mole percent P[sub 2]O[sub 5] and between about 5 and about 15 mole percent of one of PbO, BaO, and mixtures thereof. The composition, which may also include between 0 and about 5 mole percent Fe[sub 2]O[sub 3] and between 0 and about 10 mole percent B[sub 2]O[sub 3], has a thermal expansion coefficient in a range of between about 160 and 210[times]10[sup [minus]7]/C and a dissolution rate in a range of between about 2[times]10[sup [minus]7] and 2[times]10[sup [minus]9]g/cm[sup 2]-min. This composition is suitable to hermetically seal to metallic electrical components which will be subjected to humid environments over an extended period of time.

  15. Safeguards sealing systems for Zebra

    International Nuclear Information System (INIS)

    Ingram, G.; Jamieson, G.R.

    1983-01-01

    A relatively simple design has been produced for safeguards seals to be applied throughout the fuel containing areas at Zebra. It is based on the use of wire seals and regular Inspector surveillance. The application of the system would allow an Inspector to establish to a high degree of confidence that significant quantities of fuel had not been diverted during an intensive experimental programme. It would add about 5% to the time required for experiments, and careful planning would reduce this value. The inspection effort required to witness element movements during the experimental programme would average about 2 hours per day, with a further 2 hours spent each week on NDA of the fuel exposed. The Safeguards Inspector would require to spend about 25% of his time in the reactor area and would have ample time to deal with the relatively small number of fuel movements taking place in the storage area and with his duties elsewhere in the plant. During a core change, full-time inspection effort would be required for about 6 weeks each year. (author)

  16. Alfalfa seedlings grown outdoors are more resistant to UV-induced DNA damage than plants grown in a UV-free environmental chamber

    International Nuclear Information System (INIS)

    Takayanagi, Shinnosuke; Trunk, J.G.; Sutherland, J.C.; Sutherland, B.M.

    1994-01-01

    The relative UV sensitivities of alfalfa seedlings grown outdoors versus plants grown in a growth chamber under UV-filtered cool white fluorescent bulbs have been determined using three criteria: (1) level of endogenous DNA damage as sites for the UV endonuclease from Micrococcus luteus, (2) susceptibility to pyrimidine dimer induction by a UV challenge exposure and (3) ability to repair UV-induced damage. We find that outdoor-grown plants contain approximately equal frequencies of endogenous DNA damages, are less susceptible to dimer induction by a challenge exposure of broad-spectrum UV and photorepair dimers more rapidly than plants grown in an environmental chamber under cool white fluorescent lamps plus a filter removes most UV radiation. These data suggest that plants grown in a natural environment would be less sensitive to UVB-induced damage than would be predicted on the basis of studies on plants grown under minimum UV. (author)

  17. The uvsI gene of Aspergillus nidulans required for UV-mutagenesis encodes a homolog to REV3, a subunit of the DNA polymerase zeta of yeast involved in translesion DNA synthesis.

    Science.gov (United States)

    Han, K Y; Chae, S K; Han, D M

    1998-07-01

    Defects in the uvsI gene of Aspergillus nidulans resulted in high UV sensitivity and reductions of spontaneous and UV-induced reversion of certain alleles, uvsl;uvsA double mutants exhibited high methyl methane sulfonate (MMS)-sensitivity in contrast to the slight sensitivity of the component single mutants. Using such a double mutant as recipient, a clone complementing uvsI501 has been isolated from a chromosome III specific library. The deduced amino acid sequence from the 1.1-kb sequenced region, a part of the 5.2-kb DNA fragment showing uvsI-complementing activity, had a 62% identity with REV3 of yeast. Disruptants of the cloned gene demonstrated the same level of sensitivity to UV light as uvsI and failed to complement uvsI501 in heterozygous diploids.

  18. A magnetic fluid seal for rotary blood pumps: effects of seal structure on long-term performance in liquid.

    Science.gov (United States)

    Mitamura, Yoshinori; Takahashi, Sayaka; Amari, Shuichi; Okamoto, Eiji; Murabayashi, Shun; Nishimura, Ikuya

    2011-03-01

    A magnetic fluid (MF) seal enables mechanical contact-free rotation of the shaft and hence has excellent durability. The performance of an MF seal, however, has been reported to decrease in liquids. We developed an MF seal that has a "shield" mechanism, and a new MF with a higher magnetization of 47.9 kA/m. The sealing performance of the MF seal installed in a rotary blood pump was studied. Three types of MF seals were used. Seal A was a conventional seal without a shield. Seal B had the same structure as that of Seal A, but the seal was installed at 1 mm below liquid level. Seal C was a seal with a shield and the MF was set at 1 mm below liquid level. Seal A failed after 6 and 11 days. Seal B showed better results (20 and 73 days). Seal C showed long-term durability (217 and 275 days). The reason for different results in different seal structures was considered to be different flow conditions near the magnetic fluid. Fluid dynamics near the MF in the pump were analyzed using computational fluid dynamics (CFD) software. We have developed an MF seal with a shield that works in liquid for >275 days. The MF seal is promising as a shaft seal for rotary blood pumps.

  19. AlN ceramics as a detector for UV exposure

    International Nuclear Information System (INIS)

    Trinkler, L.; Berzina, B.; Boetter-Jensen, L.; Christensen, P.; Palcevskis, E.

    1999-01-01

    AlN-Y 2 O 3 ceramics is proposed for application in the field of UV detection and dosimetry. Both thermoluminescence (TL) and optically stimulated luminescence (OSL) signals from the material have been studied after exposure to UV light. AlN-Y 2 O 3 ceramics demonstrates very high sensitivity to UV light over a broad spectral region. The TL is characterized by a linear dose dependence over a large range. The fading rate of the UV-induced TL and OSL signals on storage at room temperature is lower than in the case of exposure to ionizing irradiation. (au)

  20. Cover gas seals. 11 - FFTF-LMFBR seal-test program, January-March 1974

    International Nuclear Information System (INIS)

    Kurzeka, W.; Oliva, R.; Welch, F.

    1974-01-01

    The objectives of this program are to: (1) conduct static and dynamic tests to demonstrate or determine the mechanical performance of full-size (cross section) FFTF fuel transfer machine and reactor vessel head seals intended for use in a sodium vapor - inert gas environment, (2) demonstrate that these FFTF seals or new seal configuration provide acceptable fission product and cover gas retention capabilities at LMFBR Clinch River Plant operating environmental conditions other than radiation, and (3) develop improved seals and seal technology for the LMFBR Clinch River Plant to support the national objective to reduce all atmospheric contaminations to low levels

  1. Phocine Distemper in German Seals, 2002

    Science.gov (United States)

    Wohlsein, Peter; Beineke, Andreas; Haas, Ludwig; Greiser-Wilke, Irene; Siebert, Ursula; Fonfara, Sonja; Harder, Timm; Stede, Michael; Gruber, Achim.D.; Baumgärtner, Wolfgang

    2004-01-01

    Approximately 21,700 seals died during a morbillivirus epidemic in northwestern Europe in 2002. Phocine distemper virus 1 was isolated from seals in German waters. The sequence of the P gene showed 97% identity with the Dutch virus isolated in 1988. There was 100% identity with the Dutch isolate from 2002 and a single nucleotide mismatch with the Danish isolate. PMID:15200869

  2. Electron beam selectively seals porous metal filters

    Science.gov (United States)

    Snyder, J. A.; Tulisiak, G.

    1968-01-01

    Electron beam welding selectively seals the outer surfaces of porous metal filters and impedances used in fluid flow systems. The outer surface can be sealed by melting a thin outer layer of the porous material with an electron beam so that the melted material fills all surface pores.

  3. Earlier pupping in harbour seals, Phoca vitulina

    NARCIS (Netherlands)

    Reijnders, P.J.H.; Brasseur, S.M.J.M.; Meesters, H.W.G.

    2010-01-01

    The annual reproductive cycle of most seal species is characterized by a tight synchrony of births. Typically, timing of birth shows little inter-annual variation. Here, however we show that harbour seals Phoca vitulina from the Wadden Sea (southeast North Sea) have shortened their yearly cycle,

  4. Performance evaluation of chip seals in Idaho.

    Science.gov (United States)

    2010-08-01

    The intent of this research project is to identify a wide variety of parameters that influence the performance of pavements treated via chip seals within the State of Idaho. Chip sealing is currently one of the most popular methods of maintenance for...

  5. 39 CFR 2.4 - Seal.

    Science.gov (United States)

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Seal. 2.4 Section 2.4 Postal Service UNITED STATES... Secretary of State, and is required by 39 U.S.C. 207 to be judicially noticed. The Seal shall be in the... description of the Postal Service emblem is described at 39 CFR 221.7. ...

  6. Floating seal system for rotary devices

    Science.gov (United States)

    Banasiuk, H.A.

    1983-08-23

    This invention relates to a floating seal system for rotary devices to reduce gas leakage around the rotary device in a duct and across the face of the rotary device to an adjacent duct. The peripheral seal bodies are made of resilient material having a generally U-shaped cross section wherein one of the legs is secured to a support member and the other of the legs forms a contacting seal against the rotary device. The legs of the peripheral seal form an extended angle of intersection of about 10[degree] to about 30[degree] in the unloaded condition to provide even sealing forces around the periphery of the rotary device. The peripheral seal extends around the periphery of the support member except where intersected by radial seals which reduce gas leakage across the face of the rotary device and between adjacent duct portions. The radial seal assembly is fabricated from channel bars, the smaller channel bar being secured to the divider of the support member and a larger inverted rigid floating channel bar having its legs freely movable over the legs of the smaller channel bar forming therewith a tubular channel. A resilient flexible tube is positioned within the tubular channel for substantially its full length to reduce gas leakage across the tubular channel. A spacer extends beyond the face of the floating channel near each end of the floating channel a distance to provide desired clearance between the floating channel and the face of the rotary device. 5 figs.

  7. EXPERIMENTAL STUDY OF MAGNETIC FLUID SEAL

    Directory of Open Access Journals (Sweden)

    V. G. Bashtovoi

    2006-01-01

    Full Text Available Dependences of critical pressure drop, being held by magnetic fluid seal, on time in a static state and shaft rotation velocity in dynamics have been experimentally determined. The significant influence of particles’ redistribution in magnetic fluid on static parameters of magnetic fluid seal has been established.

  8. Molybdenum sealing glass-ceramic composition

    International Nuclear Information System (INIS)

    Eagan, R.J.

    1976-01-01

    A glass-ceramic composition is described having low hydrogen and helium permeability properties, along with high fracture strength, and a thermal coefficient of expansion similar to that of molybdenum. The composition is adaptable for hermetically sealing to molybdenum at temperatures between 900 and about 950 0 C to form a hermetically sealed insulator body

  9. Sealing a Loosely Fitting Valve Assembly

    Science.gov (United States)

    Goff, L.; Tellier, G.

    1986-01-01

    Double-ring seal avoids expense of remachining or redesigning valve parts. Mating fittings on valve sealed by pair of rings - one O-ring and backup ring. Backup ring fills relatively large gap between parts. Prevents softer O-ring from being pushed into and through gap.

  10. 19 CFR 101.7 - Customs seal.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Customs seal. 101.7 Section 101.7 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY GENERAL PROVISIONS § 101.7 Customs seal. (a) Design. According to the design furnished by the Department...

  11. Dura Seal recommendations for nuclear power plants

    International Nuclear Information System (INIS)

    Anon.

    1980-01-01

    Reactor systems (BWR, PWR and Candu) are briefly reviewed with reference to the pumping services encountered in each system, to indicate the conditions imposed on mechanical seals for nuclear power plant liquid handling equipment. A description of the Dura Seals used in each service is included. (U.K.)

  12. Sealing Occlusal Dentin Caries in Permanent Molars

    DEFF Research Database (Denmark)

    Qvist, Vibeke; Borum, Mette Kit; Møller, Kirsten Dynes

    2017-01-01

    The purpose of this study was to investigate the possibility of postponing restorative intervention of manifest occlusal caries in young, permanent dentition by non-invasive sealing. This RCT-designed study included 521 occlusal lesions in 521 patients aged 6 to 17 y. Based on clinical and radiog......The purpose of this study was to investigate the possibility of postponing restorative intervention of manifest occlusal caries in young, permanent dentition by non-invasive sealing. This RCT-designed study included 521 occlusal lesions in 521 patients aged 6 to 17 y. Based on clinical...... longevity of sealings and restorations until retreatments, and 3) compare effectiveness of sealings and restorations to halt caries progression in sealed lesions and beneath restorations. Furthermore, we aimed to identify factors influencing longevity and the effectiveness of sealings and restorations.......001). The median survival time for sealings not replaced by restorations was 7.3 y (CI, 6.4 y to NA). Survival of sealings was increased in patients with low caries risk and/or excellent oral hygiene, second molars compared with first molars, and lesions not extending the middle one-third of the dentin. Survival...

  13. Machine Vision Inspection of Polymeric Traypack Seal Areas

    National Research Council Canada - National Science Library

    Coburn, John

    2002-01-01

    .... Since entrapped matter can lead to open seals, defects, and seal anomalies, a method of measurement for seal area contamination is useful in quantifying effects of filler changes, line speeds, and product formulations...

  14. Sealing of boreholes using natural, compatible materials: Granular salt

    International Nuclear Information System (INIS)

    Finley, R.E.; Zeuch, D.H.; Stormont, J.C.; Daemen, J.J.K.

    1994-01-01

    Granular salt can be used to construct high performance permanent seals in boreholes which penetrate rock salt formations. These seals are described as seal systems comprised of the host rock, the seal material, and the seal rock interface. The performance of these seal systems is defined by the complex interactions between these seal system components through time. The interactions are largely driven by the creep of the host formation applying boundary stress on the seal forcing host rock permeability with time. The immediate permeability of these seals is dependent on the emplaced density. Laboratory test results suggest that careful emplacement techniques could results in immediate seal system permeability on the order of 10 -16 m 2 to 10 -18 m 2 (10 -4 darcy to 10 -6 ). The visco-plastic behavior of the host rock coupled with the granular salts ability to ''heal'' or consolidate make granular salt an ideal sealing material for boreholes whose permanent sealing is required

  15. Active Infrared Thermography for Seal Contamination Detection in Heat-Sealed Food Packaging

    Directory of Open Access Journals (Sweden)

    Karlien D’huys

    2016-11-01

    Full Text Available Packaging protects food products from environmental influences, assuring quality and safety throughout shelf life if properly performed. Packaging quality depends on the quality of the packaging material and of the closure or seal. A common problem possibly jeopardizing seal quality is the presence of seal contamination, which can cause a decreased seal strength, an increased packaging failure risk and leak formation. Therefore, early detection and removal of seal contaminated packages from the production chain is crucial. In this work, a pulsed-type active thermography method using the heated seal bars as an excitation source was studied for detecting seal contamination. Thermal image sequences of contaminated seals were recorded shortly after sealing. The detection performances of six thermal image processing methods, based on a single frame, a fit of the cooling profiles, thermal signal reconstruction, pulsed phase thermography, principal component thermography and a matched filter, were compared. High resolution digital images served as a reference to quantify seal contamination, and processed thermal images were mapped to these references. The lowest detection limit (equivalent diameter 0.60 mm was obtained for the method based on a fit of the cooling profiles. Moreover, the detection performance of this method did not depend strongly on the time after sealing at which recording of the thermal images was started, making it a robust and generally applicable method.

  16. Sealed ion accelerator tubes (survey)

    International Nuclear Information System (INIS)

    Voitsik, L.R.

    1985-01-01

    The first publications on developing commercial models of small-scale sealed accelerator tubes in which neutrons are generated appeared in the foreign press in 1954 to 1957; they were very brief and were advertising-oriented. The tubes were designed for neutron logging of oil wells instead of ampule neutron sources (Po + Be, Ra + Be). Later, instruments of this type began to be called neutron tubes from the resulting neutron radiation that they gave off. In Soviet Union a neutron tube was developed in 1958 in connection with the development of the pulsed neutron-neutron method of studying the geological profile of oil wells. At that time the tube developed was intended, in the view of its inventors, to replace standard isotope sources with constant neutron yield. A fairly detailed survey of neutron tubes was made in the studies. 8 refs., 8 figs

  17. PWR thermocouple mechanical sealing structure

    International Nuclear Information System (INIS)

    Shen Qiuping; He Youguang

    1991-08-01

    The PWR in-core temperature detection device, which is one of measures to insure reactor safety operation, is to monitor and diagnose reactor thermal power output and in-core power distribution. The temperature detection device system uses thermocouples as measuring elements with stainless steel protecting sleeves. The thermocouple has a limited service time and should be replaced after its service time has reached. A new sealing device for the thermocouples of reactor in-core temperature detection system has been developed to facilitate replacement. The structure is complete tight under high temperature and pressure without any leakage and seepage, and easy to be assembled or disassembled in radioactive environment. The device is designed to make it possible to replace the thermocouple one by one if necessary. This is a new, simple and practical structure

  18. UV inactivation: Combined effects of UV radiation and xenobiotics in two strains of Saccharomyces

    International Nuclear Information System (INIS)

    Lochmann, E.R.; Lochmann, G.

    1997-01-01

    The effects of eight chemicals on the inactivation rate of ultraviolet radiation on the colony building capabilities of two strains of Saccharomyces cervisae - a wild type strain and a mutant deficient in excision repair - were studied. The insecticide methoxychlor, the herbicide 2,4-dichlorophenoxyacetic acid, the fungicide pentachlorophenol and its metabolite tetrachlorohydroquinone, as well as the chemicals acrylonitrile and 2,3-dichloro-1-propene have no significant impact on the effects of UV radiation in Saccharomyces cerevisae. Depending on the concentration, trichloroethylene increases the sensitivity to UV radiation. The herbicide paraquat provides efficient protection against UV radiation at concentrations where a toxic effect cannot be observed even without UV. The results were rather similar for both strains. (orig.) [de

  19. Study of UV-mutagenesis in Bacillus subtilis

    International Nuclear Information System (INIS)

    Lotareva, O.V.; Filippov, V.D.

    1974-01-01

    The sensitivity of Bac. subtilis to the inactivating and mutagenic effects of UV-mutants has been determined: uvr, which does not extract pyrimidine dimers from damaged DNA; recsub(x), which exhibits a reduced activity of ATP-dependent DNAase; poll, which is devoid of DNA polymerase, and wild strains (DT). The sensitivity of these strains to the inactivating effects of UV rays increases in the order: DT<= recsub(x) << uvr < poll, and UV mutability in the order: DT = rec(sub(x) < poll<< uvr. A comparison of UV mutagenesis in Bac. subtilis and E. coli suggests the hypothesis that the mechanisms of UV mutation formation are similar in these two organisms. (author)

  20. Susceptibility of Campylobacter jejuni and Yersinia enterocolitica to UV radiation

    Energy Technology Data Exchange (ETDEWEB)

    Butler, R.C.; Lund, V.; Carlson, D.A.

    1987-02-01

    Two enteric pathogens, Campylobacter jejuni and Yersinia enterocolitica serogroup O:3, together with Escherichia coli, were investigated for susceptibility to UV radiation at 254 nm. The UV dose required for a 3-log reduction (99.9% inactivation) of C. jejuni, Y. enterocolitica, and E. coli was 1.8, 2.7, and 5.0 mWs/cm2, respectively. Using E. coli as the basis for comparison, it appears that C. jejuni and Y. enterocolitica serogroup O:3 are more sensitive to UV than many of the pathogens associated with waterborne disease outbreaks and can be easily inactivated in most commercially available UV reactors. No association was found between the sensitivity of Y. enterocolitica to UV and the presence of a 40- to 50-megadalton virulence plasmid.

  1. Susceptibility of Campylobacter jejuni and Yersinia enterocolitica to UV radiation

    International Nuclear Information System (INIS)

    Butler, R.C.; Lund, V.; Carlson, D.A.

    1987-01-01

    Two enteric pathogens, Campylobacter jejuni and Yersinia enterocolitica serogroup O:3, together with Escherichia coli, were investigated for susceptibility to UV radiation at 254 nm. The UV dose required for a 3-log reduction (99.9% inactivation) of C. jejuni, Y. enterocolitica, and E. coli was 1.8, 2.7, and 5.0 mWs/cm2, respectively. Using E. coli as the basis for comparison, it appears that C. jejuni and Y. enterocolitica serogroup O:3 are more sensitive to UV than many of the pathogens associated with waterborne disease outbreaks and can be easily inactivated in most commercially available UV reactors. No association was found between the sensitivity of Y. enterocolitica to UV and the presence of a 40- to 50-megadalton virulence plasmid

  2. Study on sealing of boreholes

    International Nuclear Information System (INIS)

    1987-01-01

    A bibliographical research on the problem of the backfilling and sealing of boreholes, shafts and tunnels for radioactive waste disposal has been carried out. Various materials - both natural and artificial - like clay, industrial cement, polymer concrete, geothermical and magnesium cement have been examined. Their main physico-chemical and durability characteristics have been examined. The problem of the interaction between the sealing and the geological environment has been also dealt. The final subject discussed in the bibliography is the damage caused to the host formation by the excavation of shafts and tunnels. The laboratory tests have been performed on a natural clay and other types of material (cement grout, cement grout with expansive additive, cement mortar and remoulded clay) which have been used as plug materials. The main conclusions obtained from the tests are the following: - The permeability of the cement is lower than the permeability of the clay; - no adhesion was observed between clay and cement mortar, with or without expansive additive, when cured under different ambient conditions, but without any application of load; - When curing took place under load, good adhesion was observed between the clay and the cement mortar; - The flow of water in a specimen consisting of a clay core surrounded by remoulded clay is larger than in the natural clay. These results seem to be caused by the different permeabilities of the remoulded and undisturbed clay and not to depend on flow at the contact between the two materials. A remote instrumentation package for the in situ evaluation of the performance of a plug, has been developed. In order to get rid of the uncertainty associated with the infiltration of the cables through the plug a wireless data transmission system, based on acoustic waves, has been developed

  3. Ocean climate and seal condition

    Directory of Open Access Journals (Sweden)

    Crocker Daniel E

    2005-03-01

    Full Text Available Abstract Background The condition of many marine mammals varies with fluctuations in productivity and food supply in the ocean basin where they forage. Prey is impacted by physical environmental variables such as cyclic warming trends. The weaning weight of northern elephant seal pups, Mirounga angustirostris, being closely linked to maternal condition, indirectly reflects prey availability and foraging success of pregnant females in deep waters of the northeastern Pacific. The aim of this study was to examine the effect of ocean climate on foraging success in this deep-diving marine mammal over the course of three decades, using cohort weaning weight as the principal metric of successful resource accrual. Results The mean annual weaning weight of pups declined from 1975 to the late 1990s, a period characterized by a large-scale, basin-wide warm decadal regime that included multiple strong or long-duration El Niños; and increased with a return to a cool decadal regime from about 1999 to 2004. Increased foraging effort and decreased mass gain of adult females, indicative of reduced foraging success and nutritional stress, were associated with high ocean temperatures. Conclusion Despite ranging widely and foraging deeply in cold waters beyond coastal thermoclines in the northeastern Pacific, elephant seals are impacted significantly by ocean thermal dynamics. Ocean warming redistributes prey decreasing foraging success of females, which in turn leads to lower weaning mass of pups. Annual fluctuations in weaning mass, in turn, reflect the foraging success of females during the year prior to giving birth and signals changes in ocean temperature cycles.

  4. A hybrid floating brush seal (HFBS) for improved sealing and wear performance in turbomachinery applications

    Science.gov (United States)

    Lattime, Scott Byran

    A conceptually new type of seal has been developed for gas turbine applications which dramatically reduces wear and leakage associated with current labyrinth and brush seal technologies. The Hybrid Floating Brush Seal (HFBS) combines brush seal and film riding face seal technologies to create a hybrid seal that allows both axial and radial excursions of the sealed shaft, while simultaneously eliminating interface surface speeds (friction and heat) between the rotor and the brush material that characterize standard brush seal technology. A simple test rig was designed to evaluate feasibility of the HFBS under relatively low pressures and rotational speeds (50psig, 5krpm). A second test stand was created to study the effects of centrifugal force on bristle deflection. A third test facility was constructed for prototype development and extensive room temperature testing at moderate pressures and fairly high rotational speeds (100psig, 40krpm). This test rig also allowed the evaluation of the HFBS during axial movement of a rotating shaft. An analytical model to predict the effects of centrifugal force on the bristles of a rotating brush seal was developed. Room temperature analysis of the HFBS proved successful for relatively high operating rotational velocities at moderate pressures with very acceptable leakage rates for gas turbine engines. Brush seals were able to track rotor speeds up to 24krpm while maintaining sealing integrity. The HFBS's ability to function under axial shaft displacement and synchronous dynamic radial loading was also proven successful. Hydrodynamic performance of the face seal was proven to provide adequate stiffness and load carrying capacity to keep the brush seal from contacting the face seal at pressure drops across the brush of up to 100psi. Leakage performance over standard brush seal and labyrinth technology was quite dramatic. The HFBS showed its sealing advantage using much higher radial interference between the rotor and the bristle

  5. Does the seal licensing system in Scotland have a negative impact on seal welfare?

    Directory of Open Access Journals (Sweden)

    Laetitia Nunny

    2016-08-01

    Full Text Available This study examined the licensing system that permits seal shooting in Scotland, which was established under Part 6 Conservation of Seals of the Marine (Scotland Act 2010. Four approaches were used: data were collated and analyzed from both the Scottish Government and Scottish Marine Animal Stranding Scheme; a survey was sent to current license holders and informal interviews were conducted with key stakeholder types. Between February 2011 and the end of October 2015, 1229 gray seals and 275 common seals were reported shot under license to the Scottish Government. The numbers of seals reported as shot has reduced year-on-year since the licensing system was put in place. While some license holders, notably fish farms, were using some non-lethal forms of deterrent to reduce seal-related damage, these were often used alongside seal shooting. Of the seals reported as shot to the Scottish Government, only a small percentage were also reported to the Scottish Marine Animal Stranding Scheme, despite this being a licensing requirement. Only 2.3% of the shot gray seals and 4.5% of the shot common seals were necropsied. There is evidence from these necropsies that some seals had not died instantly or had not been shot in the manner recommended by the Scottish Seal Management Code of Practice. These preliminary results show that more carcasses need to be recovered and necropsied if the welfare implications of current seal shooting practice are to be properly assessed. The current legislation does not specify closed seasons to protect breeding seals and thirty-five per cent of necropsied seals were pregnant gray seals. Seals have also been shot during their lactation periods when pups are dependent on their mothers. This raises significant welfare concerns. The re-introduction of closed seasons specific to each species of seal is recommended along with greater effort to deploy non-lethal methods. Independent assessment of the number of seals being killed

  6. Feasibility of using microencapsulated phase change materials as filler for improving low temperature performance of rubber sealing materials.

    Science.gov (United States)

    Tiwari, Avinash; Shubin, Sergey N; Alcock, Ben; Freidin, Alexander B; Thorkildsen, Brede; Echtermeyer, Andreas T

    2017-11-01

    The feasibility of a novel composite rubber sealing material to improve sealing under transient cooling (in a so-called blowdown scenario) is investigated here. A composite of hydrogenated nitrile butadiene rubber (HNBR) filled with Micro Encapsulated Phase Change Materials (MEPCM) is described. The fillers contain phase change materials that release heat during the phase transformation from liquid to solid while cooling. This exotherm locally heats the rubber and may improve the function of the seal during a blowdown event. A representative HNBR-MEPCM composite was made and the critical thermal and mechanical properties were obtained by simulating the temperature distribution during a blowdown event. Simulations predict that the MEPCM composites can delay the temperature decrease in a region of the seal during the transient blowdown. A sensitivity analysis of material properties is also presented which highlights possible avenues of improvement of the MEPCMs for sealing applications.

  7. Analysis on the Viscous Pumping in a Magnetic Fluid Seal Under a Rotating Load and the Seal Design

    OpenAIRE

    長屋, 幸助; 大沼, 浩身; 佐藤, 淳

    1990-01-01

    This paper discusses effects of viscous pumping in a magnetic fluid seal under a rotating load. The Reynolds equation was presented for the seal based on magnetic fluid mechanics, and the expressions for obtaining pressures in the seal, eccentricities of the rotating shaft due to the viscous pumping and seal pressures were given. Numerical Calculations were carried out for some sample problems, and the effect of magnetic flux densities on the pressure in the seal and the seal pressures were c...

  8. UV-induced structural changes in chromatin

    International Nuclear Information System (INIS)

    Lang, H.; Zimmer, C.; Vengerov, Yu.Yu.

    1985-01-01

    UV-induced structural alterations of chromatin were studied by means of CD, electron microscopic, and gel electrophoretic measurements. The results indicate that chromatin undergoes serious structural changes after irradiation even at very low fluences. In the low fluence range the structural transitions from the higher ordered chromatin structure to the unfolded state occur without detectable changes in the content of histone H1 and of the core histones. Histone H1 disappears only at fluences above 10 kJ/m 2 . Furthermore, DNA in chromatin is much more sensitive against UV-irradiation and shows a higher degree of strand scission relative to free DNA. While fragmentation in free DNA occurs at fluences above 15 kJ/m 2 , it occurs even at 5.5 kJ/m 2 in the case of chromatin. The biological meaning of the observed UV-induced structural alterations of chromatin is discussed. (author)

  9. Photoreactivation of UV-irradiated Legionella pneumophila and other Legionella species

    International Nuclear Information System (INIS)

    Knudson, G.B.

    1985-01-01

    Shortwave UV light was assessed as a feasible modality for the control of Legionnaires disease bacterium in water. The results of this study show that Legionella pneumophila and six other Legionella species are very sensitive to low doses of UV. However, all Legionella species tested effectively countered the germicidal effect of UV when subsequently exposed to photoreactivating light

  10. UV Fluorescence Photography of Works of Art : Replacing the Traditional UV Cut Filters with Interference Filters

    Directory of Open Access Journals (Sweden)

    Luís BRAVO PEREIRA

    2010-09-01

    Full Text Available For many years filters like the Kodak Wratten E series, or the equivalent Schneider B+W 415, were used as standard UV cut filters, necessary to obtain good quality on UV Fluorescence photography. The only problem with the use of these filters is that, when they receive the UV radiation that they should remove, they present themselves an internal fluorescence as side effect, that usually reduce contrast and quality on the final image. This article presents the results of our experiences on using some innovative filters, that appeared available on the market in recent years, projected to adsorb UV radiation even more efficiently than with the mentioned above pigment based standard filters: the interference filters for UV rejection (and, usually, for IR rejection too manufactured using interference layers, that present better results than the pigment based filters. The only problem with interference filters type is that they are sensitive to the rays direction and, because of that, they are not adequate to wide-angle lenses. The internal fluorescence for three filters: the B+W 415 UV cut (equivalent to the Kodak Wratten 2E, pigment based, the B+W 486 UV IR cut (an interference type filter, used frequently on digital cameras to remove IR or UV and the Baader UVIR rejection filter (two versions of this interference filter were used had been tested and compared. The final quality of the UV fluorescence images seems to be of a superior quality when compared to the images obtained with classic filters.

  11. Design and development of face seal type sealing plug for advanced heavy water reactor

    International Nuclear Information System (INIS)

    Bansal, S.; Bhattacharyya, S.; Patel, R.J.; Agrawal, R.G.; Vaze, K.K.

    2005-09-01

    Advanced Heavy Water Reactor is a vertical pressure tube type reactor having light water as its coolant and heavy water as moderator. Sealing plug is required to close the pressure boundary of main heat transport system of the reactor by preventing escape of light water/steam From the coolant channel. There are 452 coolant channels in the reactor located in square lattice pitch. Sealing plug is located at the top of each coolant channel (in the top end fitting). Top end fitting is having a stepped bore to create a sealing face. Sealing plug is held through its expanded jaws in a specially provided groove of the end fitting. The plug was designed and prototypes were manufactured considering its functional importance, intricate design and precision machining requirements. Sealing plug consists of about 20 components mostly made up of precipitation hardening stainless steel, which is suitable for water environment and meets other requirements of strength and resistance to wear and galling. Seal disc is a critical component of the sealing plug as it is the pressure-retaining component. It is a circular disc with protruded stem. One face of the seal disc is nickel plated in the peripheral area that creates the sealing by abutting against the sealing face provided in the end fitting. The typical shape and profile of seal disc provides flexibility and allows elastic deformation to assist in locking of sealing plug and creating adequate seating force for effective sealing. Design and development aspects of the sealing plug have been detailed out in this report. Also results of stress analysis and experimental studies for seal disc have been mentioned in the report. Stress analysis and experimental testing was required for the seal disc because high stresses are developed due to its exposure to high pressure and temperature environment of Main Heat Transport system. Hot testing was carried out to simulate the reactor-simulated condition. The performance was found to be

  12. Sealing glasses for titanium and titanium alloys

    Science.gov (United States)

    Brow, Richard K.; McCollister, Howard L.; Phifer, Carol C.; Day, Delbert E.

    1997-01-01

    Barium lanthanoborate sealing-glass compositions are provided comprising various combinations (in terms of mole-%) of boron oxide (B.sub.2 O.sub.3), barium oxide (BaO), lanthanum oxide (La.sub.2 O.sub.3), and at least one other oxide selected from the group consisting of aluminum oxide (Al.sub.2 O.sub.3), calcium oxide (CaO), lithium oxide (Li.sub.2 O), sodium oxide (Na.sub.2 O), silicon dioxide (SiO.sub.2), or titanium dioxide (TiO.sub.2). These sealing-glass compositions are useful for forming hermetic glass-to-metal seals with titanium and titanium alloys having an improved aqueous durability and favorable sealing characteristics. Examples of the sealing-glass compositions are provided having coefficients of thermal expansion about that of titanium or titanium alloys, and with sealing temperatures less than about 900.degree. C., and generally about 700.degree.-800.degree. C. The barium lanthanoborate sealing-glass compositions are useful for components and devices requiring prolonged exposure to moisture or water, and for implanted biomedical devices (e.g. batteries, pacemakers, defibrillators, pumps).

  13. Numerical Investigation of the Effect of Radial Lip Seal Geometry on Sealing Performance

    Science.gov (United States)

    Tok, G.; Parlar, Z.; Temiz, V.

    2018-01-01

    Sealing elements are often needed in industry and especially in machine design. With the change and development of machine technology from day to day, sealing elements show continuous development and change in parallel with these developments. Many factors influence the performance of the sealing elements such as shaft surface roughness, radial force, lip geometry etc. In addition, the radial lip seals must have a certain pre-load and interference in order to provide a good sealing. This also affects the friction torque. Researchers are developing new seal designs to reduce friction losses in mechanical systems. In the presented study, the effect of the lip seal geometry on sealing performance will be examined numerically. The numerical model created for this purpose will be verified with experimental data firstly. In the numerical model, shaft and seal will be modeled as hyper-elastic in 2D and 3D. NBR (Nitrile Butadiene Rubber) as seal material will be analyzed for the rotating shaft state at constant speed by applying a uniform radial force.

  14. Tamper-indicating quantum optical seals

    Energy Technology Data Exchange (ETDEWEB)

    Humble, Travis S [ORNL; Williams, Brian P [ORNL

    2015-01-01

    Confidence in the means for identifying when tampering occurs is critical for containment and surveillance technologies. Fiber-optic seals have proven especially useful for actively surveying large areas or inventories due to the extended transmission range and flexible layout of fiber. However, it is reasonable to suspect that an intruder could tamper with a fiber-optic sensor by accurately replicating the light transmitted through the fiber. In this contribution, we demonstrate a novel approach to using fiber-optic seals for safeguarding large-scale inventories with increased confidence in the state of the seal. Our approach is based on the use of quantum mechanical phenomena to offer unprecedented surety in the authentication of the seal state. In particular, we show how quantum entangled photons can be used to monitor the integrity of a fiber-optic cable - the entangled photons serve as active sensing elements whose non-local correlations indicate normal seal operation. Moreover, we prove using the quantum no-cloning theorem that attacks against the quantum seal necessarily disturb its state and that these disturbances are immediately detected. Our quantum approach to seal authentication is based on physical principles alone and does not require the use of secret or proprietary information to ensure proper operation. We demonstrate an implementation of the quantum seal using a pair of entangled photons and we summarize our experimental results including the probability of detecting intrusions and the overall stability of the system design. We conclude by discussing the use of both free-space and fiber-based quantum seals for surveying large areas and inventories.

  15. Reactor coolant pump seal leakage monitoring

    International Nuclear Information System (INIS)

    Stevens, D.M.; Spencer, J.W.; Morris, D.J.; James, W.; Shugars, H.G.

    1986-01-01

    Problems with reactor coolant pump seals have historically accounted for a large percentage of unscheduled outages. Studies performed for the Electric Power Research Institute (EPRI) have shown that the replacement of coolant pump seals has been one of the leading causes of nuclear plant unavailability over the last ten years. Failures of coolant pump seals can lead to primary coolant leakage rates of 200-500 gallons per minute into the reactor building. Airborne activity and high surface contamination levels following these failures require a major cleanup effort and increases the time and personnel exposure required to refurbish the pump seals. One of the problems in assessing seal integrity is the inability to accurately measure seal leakage. Because seal leakage flow is normally very small, it cannot be sensed directly with normal flow instrumentation, but must be inferred from several other temperature and flow measurements. In operating plants the leakage rate has been quantified with a tipping-bucket gauge, a device which indicates when one quart of water has been accumulated. The tipping-bucket gauge has been used for most rainfall-intensity monitoring. The need for a more accurate and less expensive gauge has been addressed. They have developed a drop-counter precipitation sensor has been developed and optimized. The applicability of the drop-counter device to the problem of measuring seal leakage is being investigated. If a review of system specification and known drop-counter performance indicates that this method is feasible for measuring seal leak rates, a drop-counter gauge will be fabricated and tested in the laboratory. If laboratory tests are successful the gauge will be demonstrated in a pump test loop at Ontario Hydro and evaluated under simulated plant conditions. 3 references, 2 figures

  16. Vortex Shedding in the Wake Induced by a Real Elephant Seal Whisker

    Science.gov (United States)

    Turk, Jodi; Omilion, Alexis; Zhang, Wei; Kim, Jeong-Jae; Kim, Jeong-Ju; Choi, Woo-Rak; Lee, Sang-Joon

    2017-11-01

    Biomimicry has been adopted to create innovative solutions in a vast range of applications. One such application is the design of seal-whisker-inspired flow sensors for autonomous underwater vehicles (AUVs). In dark, cramped, and unstable terrain AUVs are not able to maneuver using visual and sonar-based navigation. Hence, it is critical to use underwater flow sensors to accurately detect minute disturbances in the surroundings. Certain seal whiskers exhibit a unique undulating three-dimensional morphology that can reduce vortex induced vibrations (VIVs) if the major axis of the whisker cross-section is aligned to the inflow. This allows the seal to precisely track prey fish upstream using solely their whiskers. The current study aims to understand the effect of a real seal whisker's morphology on the vortex shedding behavior. Despite extensive studies of wake induced by scaled whisker-like models, the vortex shedding in the wake of a real seal whisker is not well understood. A series of experiments are conducted with a high-speed Particle Imaging Velocimetry (PIV) system in a water channel to examine the vortex shedding downstream from a smooth whisker and an undulating whisker at a Reynolds number of a few hundred. Results of the vortex shedding induced by real seal whiskers can provide insights on developing high-sensitivity underwater flow sensors for AUVs and other whisker-inspired structures.

  17. A graphite oxide (GO)-based remote readable tamper evident seal

    International Nuclear Information System (INIS)

    Cattaneo, A; Marchi, A N; Farrar, C R; Mascareñas, D D L; Bossert, J A; Gupta, G; Mohite, A; Dumont, J H; Purdy, G M; Guzman, C; Haaker, A; Miller, K A

    2015-01-01

    This paper presents a prototype of a remotely readable graphite oxide (GO) paper-based tamper evident seal. The proposed device combines the tunable electrical properties offered by reduced graphite oxide (RGO) with a compressive sampling scheme. The benefit of using RGO as a tamper evident seal material is the sensitivity of its electrical properties to the common mechanisms adopted to defeat tamper-evident seals. RGO’s electrical properties vary upon local stress or cracks induced by mechanical action (e.g., produced by shimming or lifting attacks). Further, modification of the seal’s electrical properties can result from the incidence of other defeat mechanisms, such as temperature changes, solvent treatment and steam application. The electrical tunability of RGO enables the engraving of a circuit on the area of the tamper evident seal intended to be exposed to malicious attacks. The operation of the tamper evident seal, as well as its remote communication functionality, is supervised by a microcontroller unit (MCU). The MCU uses the RGO-engraved circuitry to physically implement a compressive sampling acquisition procedure. The compressive sampling scheme provides the seal with self-authentication and self-state-of-health awareness capabilities. The prototype shows potential for use in low-power, embedded, remote-operation non-proliferation security related applications. (paper)

  18. The impact of glide phases on the trackability of hydrodynamic trails in harbour seals (Phoca vitulina).

    Science.gov (United States)

    Wieskotten, S; Dehnhardt, G; Mauck, B; Miersch, L; Hanke, W

    2010-11-01

    The mystacial vibrissae of harbour seals (Phoca vitulina) constitute a highly sensitive hydrodynamic receptor system enabling the seals to detect and follow hydrodynamic trails. In the wild, hydrodynamic trails, as generated by swimming fish, consist of cyclic burst-and-glide phases, associated with various differences in the physical parameters of the trail. Here, we investigated the impact of glide phases on the trackability of differently aged hydrodynamic trails in a harbour seal. As fish are not easily trained to swim certain paths with predetermined burst-and-glide phases, the respective hydrodynamic trails were generated using a remote-controlled miniature submarine. Gliding phases in hydrodynamic trails had a negative impact on the trackability when trails were 15 s old. The seal lost the generated trails more often within the transition zones, when the submarine switched from a burst to a glide moving pattern. Hydrodynamic parameter analysis (particle image velocimetry) revealed that the smaller dimensions and faster decay of hydrodynamic trails generated by the gliding submarine are responsible for the impaired success of the seal tracking the gliding phase. Furthermore, the change of gross water flow generated by the submarine from a rearwards-directed stream in the burst phase to a water flow passively dragged behind the submarine during gliding might influence the ability of the seal to follow the trail as this might cause a weaker deflection of the vibrissae. The possible ecological implications of intermittent swimming behaviour in fish for piscivorous predators are discussed.

  19. UV Catalysis, Cyanotype Photography, and Sunscreens

    Science.gov (United States)

    Lawrence, Glen D.; Fishelson, Stuart

    1999-09-01

    This laboratory experiment is intended for a chemistry course for non-science majors. The experiment utilizes one of the earliest photographic processes, the cyanotype process, to demonstrate UV catalysis of chemical reactions. In addition to making photographic prints from negatives, the process can be used to test the effectiveness of sunscreens and the relative efficacy of the SPF (sun protection factor) rating of sunscreens. This is an inexpensive process, requiring solutions of ammonium ferric citrate and potassium ferricyanide, with options to use hydrogen peroxide and ammonium hydroxide solutions. Students can prepare their own UV-sensitized paper with the indicated chemicals and watch the photographic image appear as it is exposed to sunlight or fluorescent UV lamps in a light box designed for use in this experiment. The laboratory experiment should stimulate discussion of UV catalysis, photographic processes and photochemistry, sunscreens, and UV damage to biological organisms. The chemicals used are relatively nontoxic, and the procedure is simple enough to be used by groups of diverse ages and abilities.

  20. Design, construction and monitoring of sealing work

    International Nuclear Information System (INIS)

    Fischle, W.R.; Schwieger, K.

    1986-01-01

    By flooding a worked-out mine, it was possible to test the sealing work on a full scale. The aim is to erect an impermeable, statically secure seal requiring no maintenance. Apart from testing measuring equipment, data were to be obtained for later safety calculations. The measurements are also used to check the computer programs. After a dry period of over a year, flooding started at the beginning of 1985 and so did the testing of the seal and the measuring equipment in caustic soda solution. (orig./PW) [de

  1. Magnetic fluid seals for DWDM filter manufacturing

    International Nuclear Information System (INIS)

    Li Zhixin

    2002-01-01

    Performance requirements of rotary magnetic fluid seals for thin film dense wavelength division multiplexing filter manufacturing are discussed. High speed, large diameter, tight tolerance for rotating accuracy and motion control are some of the technical challenges in this application. Analytical methods for studying these technical issues and experimental data are presented. Innovative design approaches are also discussed. It is shown that using the most advanced magnetic fluid technology and seal design technology, magnetic fluid seals can provide satisfactory performance in this challenging new application

  2. Pilot cryo tunnel: Attachments, seals, and insulation

    Science.gov (United States)

    Wilson, J. F.; Ware, G. D.; Ramsey, J. W., Jr.

    1974-01-01

    Several different tests are described which simulated the actual configuration of a cryogenic wind tunnel operating at pressures up to 5 atmospheres (507 kPa) and temperatures from -320 F (78K) to 120 F (322K) in order to determine compatible bolting, adequate sealing, and effective insulating materials. The evaluation of flange attachments (continuous threaded studs) considered bolting based on compatible flanges, attachment materials, and prescribed bolt elongations. Various types of seals and seal configurations were studied to determine suitability and reusability under the imposed pressure and temperature loadings. The temperature profile was established for several materials used for structural supports.

  3. Compliant Glass Seals for SOFC Stacks

    Energy Technology Data Exchange (ETDEWEB)

    Chou, Yeong -Shyung [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Choi, Jung-Pyung [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Xu, Wei [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Stephens, Elizabeth V. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Koeppel, Brian J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Stevenson, Jeffry W. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Lara-Curzio, Edgar [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2014-04-30

    This report summarizes results from experimental and modeling studies performed by participants in the Solid-State Energy Conversion Alliance (SECA) Core Technology Program, which indicate that compliant glass-based seals offer a number of potential advantages over conventional seals based on de-vitrifying glasses, including reduced stresses during stack operation and thermal cycling, and the ability to heal micro-damage induced during thermal cycling. The properties and composition of glasses developed and/or investigated in these studies are reported, along with results from long-term (up to 5,800h) evaluations of seals based on a compliant glass containing ceramic particles or ceramic fibers.

  4. Infiltrating/sealing proximal caries lesions

    DEFF Research Database (Denmark)

    Martignon, S; Ekstrand, K R; Gomez, J

    2012-01-01

    This randomized split-mouth controlled clinical trial aimed at assessing the therapeutic effects of infiltration vs. sealing for controlling caries progression on proximal surfaces. Out of 90 adult students/patients assessed at university clinics and agreeing to participate, 39, each with 3...... differences in lesion progression between infiltration and placebo (P = 0.0012) and between sealing and placebo (P = 0.0269). The study showed that infiltration and sealing are significantly better than placebo treatment for controlling caries progression on proximal lesions. No significant difference...

  5. Development of a simple, sensitive and inexpensive ion-pairing cloud point extraction approach for the determination of trace inorganic arsenic species in spring water, beverage and rice samples by UV-Vis spectrophotometry.

    Science.gov (United States)

    Gürkan, Ramazan; Kır, Ufuk; Altunay, Nail

    2015-08-01

    The determination of inorganic arsenic species in water, beverages and foods become crucial in recent years, because arsenic species are considered carcinogenic and found at high concentrations in the samples. This communication describes a new cloud-point extraction (CPE) method for the determination of low quantity of arsenic species in the samples, purchased from the local market by UV-Visible Spectrophotometer (UV-Vis). The method is based on selective ternary complex of As(V) with acridine orange (AOH(+)) being a versatile fluorescence cationic dye in presence of tartaric acid and polyethylene glycol tert-octylphenyl ether (Triton X-114) at pH 5.0. Under the optimized conditions, a preconcentration factor of 65 and detection limit (3S blank/m) of 1.14 μg L(-1) was obtained from the calibration curve constructed in the range of 4-450 μg L(-1) with a correlation coefficient of 0.9932 for As(V). The method is validated by the analysis of certified reference materials (CRMs). Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. UV Deprivation Influences Social UV Preference in Juvenile Sticklebacks

    Directory of Open Access Journals (Sweden)

    Ricarda Modarressie

    2015-05-01

    Full Text Available Social aggregations occur in many different animal taxa and mainly result from non-random assortment. Investigating factors that shape and maintain the composition of social aggregations are among others a main topic for understanding ecological speciation processes. Aggregation decisions are mediated by olfactory and visual cues, which in many animals are extended into the UV part of the electromagnetic spectrum. Here, we were interested in developmental plasticity of social preferences with respect to UV radiation in aquatic organisms. Specifically, we tested whether different lighting environments with respect to UV wavelengths during early life stages influence the shoaling preference in juvenile threespine sticklebacks (Gasterosteus aculeatus. Family (full-sibling groups were split and reared under UV-lacking (UV- and UV-present (UV+ lighting conditions. Subsequent shoal choice experiments, in which test fish from both rearing conditions could simultaneously choose between a shoal seen behind a UV-blocking (UV- and a shoal seen behind a UV-transmitting (UV+ filter, revealed a significant effect of lighting condition during rearing on association preference. Test fish that had been deprived of UV spent significantly more time near the UV- shoal compared to the test fish reared under full-spectrum lighting conditions. The results are discussed with respect to plasticity of the visual system and environmental lighting conditions.

  7. Effect of bar sealing parameters on OPP/MCPP heat seal strength

    Directory of Open Access Journals (Sweden)

    2007-11-01

    Full Text Available The effect of bar sealing parameters on the heat seal strength of oriented polypropylene (OPP/metallic cast polypropylene (MCPP laminate film was investigated. Based on the results obtained from the parametric study, a bar sealing process window was developed. All points drop within the process window are combinations of platen temperature and dwell time that produce acceptable heat seal. Optimum combinations are indicated by the lower border of the window. The plateau initiation temperature, Tpi of OPP/MCPP laminate film used in the present study occurred before the final melting temperature, Tmf of the sealant material. The highest achievable heat seal strength was at the plateau region, and the corresponding failure modes were delaminating, tearing or combine failure modes (delaminating and tearing. Minimum pressure level of 1.25 bars is necessary to bring the laminate interface into intimate contact in order to effect sealing.

  8. Aerial surveys of seals at Rødsand seal sanctuary and adjacent haul-out sites

    DEFF Research Database (Denmark)

    Teilmann, J.; Dietz, R.; Edren, Susi M.C.

    This report describes the preliminary results of aerial surveys at Rødsand seal sanctuary, southeast Denmark and adjacent seal haul-out sites in southwestern Baltic. The work was carried out in connection with studies of potential effects of the Nysted offshore wind farm. Rødsand seal sanctuary...... is a part of seal management area 4, and the area is believed to hold a more or less closed population with little exchange to other areas. Although the harbour seal is relatively stationary there may be movements between the haul-out sites in the area. A possible reaction to disturbance from...... the construction and operation of the wind farm may be that the seals use other haul-out sites to a higher extend....

  9. Investigating the sealing capacity of a seal system in rock salt (DOPAS project)

    Energy Technology Data Exchange (ETDEWEB)

    Jantschik, Kyra; Moog, Helge C.; Czaikowski, Oliver; Wieczorek, Klaus [Gesellschaft fuer Anlagen- und Reaktorsicherheit (GRS) gGmbH, Braunschweig (Germany)

    2016-11-15

    This paper describes research and development work on plugging and sealing repositories, an issue of fundamental importance for the rock salt option which represents one of the three European repository options, besides the clay rock and the crystalline rock options. The programme aims at providing experimental data needed for the theoretical analysis of the long-term sealing capacity of concrete- based sealing materials. In order to demonstrate hydro-mechanical material stability under representative load scenarios, a comprehensive laboratory testing programme is carried out. This comprises investigation of the sealing capacity of the combined seal system and impact of the so-called excavation-damaged zones (EDZ) as well as investigation of the hydro-chemical long-term stability of the seal in contact with different brines under diffusive and advective conditions. This paper presents experimental approaches and preliminary results from laboratory investigations on salt concrete and combined systems as obtained to date.

  10. UV spectroscopy. Ch. 16

    International Nuclear Information System (INIS)

    Stevens, Eugene S.

    1991-01-01

    The use of synchrotron radiation (SR) in the ultraviolet (UV) region by biophysics and biophysical chemists continues to increase as access improves and as awareness of its unique combination of properties grows. SR is continuously tunable, intense (even in the UV region), and pulsed. Pulse characteristics are comparable to those of picosecond lasers, but have the advantage of being independent of wavelength. Drawbacks are the continuous, but slow, decay of intensity arising from loss of particles in the ring, and the intrinsic limitation in pulse repetition rate. The particular combination of features that makes SR a superior light source depends upon the application. Spectroscopic techniques based on absorption and some techniques based on emission exploit its intensity and continuous tunability; time resolved techniques make use of its pulse characteristics. This chapter is a status report on two of the major biological applications of UV SR, circular dichroism and time-resolved fluorescence, covering mainly the published literature of the last five years. For the present purpose, the term UV is understood to include the vacuum ultraviolet region to 100 nm. (author). 83 refs.; 4 figs.; 6 tabs

  11. Experimental analysis of axial and radial stress distribution in soft materials used for petrochemical valve stem sealing package

    Science.gov (United States)

    Ripeanu, R. G.; Ispas, A.; Ispas, D.

    2017-02-01

    Paper presents experimental results obtained by authors regarding pressure values and distribution, by using a special pressure sensitive paper, type FujiFilm and adequate hardware equipment and a soft program FPD810 Win. Was obtained the proper axial force related for different materials in order to respect the valves sealing demands. Were showed that, pressure maps obtained depends of the construction of sealing package, density of the preformed band rings and corrected sealing replacing on location in case of square braided cord used.

  12. Influence of the stack length on the stresses and temperatures at the location of a borehole seal

    International Nuclear Information System (INIS)

    Beemsterboer, C.J.J.; Prij, J.

    1993-02-01

    This report deals with a numerical analysis to determine the sensitivity of the thermomechanical loading of the borehole seal with respect to the length of the stack of canisters. The analysis deals with the mechanical loads (stresses, deformation and temperature) caused by the rock pressure at the location of the borehole seal and by the heat producing canisters in the borehole. The aim of the analysis is to obtain insight in the temperature and stress load on the borehole seal and to define the distance above which these loads can be neglected

  13. Troubleshooting ProSeal LMA

    Directory of Open Access Journals (Sweden)

    Bimla Sharma

    2009-01-01

    Full Text Available Supraglottic devices have changed the face of the airway management. These devices have contributed in a big way in airway management especially, in the difficult airway scenario significantly decreasing the pharyngolaryngeal morbidity. There is a plethora of these devices, which has been well matched by their wider acceptance in clinical practice. ProSeal laryngeal mask airway (PLMA is one such frequently used device employed for spontaneous as well as controlled ventilation. However, the use of PLMAat tunes maybe associated with certain problems. Some of the problems related with its use are unique while others are akin to the classic laryngeal mask airway (eLMA. However, expertise is needed for its safe and judicious use, correct placement, recognition and management of its various malpositions and complications. The present article describes the tests employed for proper confirmation of placementto assess the ventilatooy and the drain tube functions of the mask, diagnosis of various malpositions and the management of these aspects. All these areas have been highlighted under the heading of troubleshooting PLMA. Many problems can be solved by proper patient and procedure selection, maintaining adequate depth of anaesthesia, diagnosis and management of malpositions. Proper fixation of the device and monitoring cuff pressure intraopera-tively may bring down the incidence of airway morbidity.

  14. Rapid Sampling from Sealed Containers

    International Nuclear Information System (INIS)

    Johnston, R.G.; Garcia, A.R.E.; Martinez, R.K.; Baca, E.T.

    1999-01-01

    The authors have developed several different types of tools for sampling from sealed containers. These tools allow the user to rapidly drill into a closed container, extract a sample of its contents (gas, liquid, or free-flowing powder), and permanently reseal the point of entry. This is accomplished without exposing the user or the environment to the container contents, even while drilling. The entire process is completed in less than 15 seconds for a 55 gallon drum. Almost any kind of container can be sampled (regardless of the materials) with wall thicknesses up to 1.3 cm and internal pressures up to 8 atm. Samples can be taken from the top, sides, or bottom of a container. The sampling tools are inexpensive, small, and easy to use. They work with any battery-powered hand drill. This allows considerable safety, speed, flexibility, and maneuverability. The tools also permit the user to rapidly attach plumbing, a pressure relief valve, alarms, or other instrumentation to a container. Possible applications include drum venting, liquid transfer, container flushing, waste characterization, monitoring, sampling for archival or quality control purposes, emergency sampling by rapid response teams, counter-terrorism, non-proliferation and treaty verification, and use by law enforcement personnel during drug or environmental raids

  15. HMSRP Hawaiian Monk Seals on Social Media

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — As social media platforms develop, they potentially provide valuable information for wildlife researchers and managers. NOAA’s Hawaiian Monk Seal Research Program...

  16. Multi-Canister overpack sealing configuration

    International Nuclear Information System (INIS)

    SMITH, K.E.

    1998-01-01

    The Spent Nuclear Fuel (SNF) position regarding the Multi-Canister Overpack (MCO) sealing configuration is to initially rely on an American Society of Mechanical Engineers (ASME) Section III Subsection NB code compliant mechanical closure/sealing system to quickly and safely establish and maintain full confinement of radioactive materials prior to and during MCO fuel drying activities. Previous studies have shown the mechanical seal to be the preferred closure method, based on dose, cost, and schedule considerations. The cost and schedule impacts of redesigning the mechanical closure to a welded shield plug do not support changing the closure system. The SNF Project has determined that the combined mechanical/welded closure system meets or exceeds the regulatory requirements to provide redundant seals while accommodating key safety and schedule limitations that are unique to K Basins fuel removal effort

  17. HMSRP Hawaiian Monk Seal Fisheries Interactions data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The data set contains records of all documented hookings and/or entanglements of Hawaiian monk seals with actively fished gear, both commercial and recreational. The...

  18. Temporary fire sealing of penetrations on TFTR

    International Nuclear Information System (INIS)

    Hondorp, H.L.

    1981-02-01

    The radiation shielding provided for TFTR for D-D and D-T operation will be penetrated by numerous electrical and mechanical services. Eventually, these penetrations will have to be sealed to provide the required fire resistance, tritium sealability, pressure integrity and radiation attenuation. For the initial hydrogen operation, however, fire sealing of the penetrations in the walls and floor is the primary concern. This report provides a discussion of the required and desirable properties of a temporary seal which can be used to seal these penetrations for the hydrogen operation and then subsequently be removed and replaced as required for the D-D and D-T operations. Several candidate designs are discussed and evaluated and recommendations are made for specific applications

  19. HMSRP Hawaiian Monk Seal Microsatellite Genotypes

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Currently ~2,400 Hawaiian monk seal specimens have been analyzed genetically, providing genotypes at 18 microsatellite loci. These data are organized by individual,...

  20. HMSRP Hawaiian Monk Seal Survival Factors

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains records of survival factors recorded by PSD personnel and cooperating scientists as part of the ongoing monk seal population assessment...

  1. Cask systems development program seal technology

    International Nuclear Information System (INIS)

    Madsen, M.M.; Humphreys, D.L.; Edwards, K.R.

    1991-01-01

    General design or test performance requirements for radioactive materials (RAM) packages are specified in Title 10 of the US Code of Federal Regulations Part 71 (10 CFR 71). Seals that provide the containment system interface between the packaging body and the closure must function in both high- and low-temperature environments under dynamic and static conditions. Experiments were performed to characterize the performance of several seal materials at low temperatures. Helium leak tests on face seals were used to compare the materials. Materials tested include butyl, neoprene, ethylene propylene, fluorosilicone, silicone, Eypel, Kalrez, Teflon, fluorocarbon, and Teflon/silicone composites. Results show that the seal materials tested, with the exception of silicone S613-60, are not leak tight (1 x 10 -7 std cm 3 /s) at manufacturer low-temperature ratings

  2. Magnetic vector field tag and seal

    Science.gov (United States)

    Johnston, Roger G.; Garcia, Anthony R.

    2004-08-31

    One or more magnets are placed in a container (preferably on objects inside the container) and the magnetic field strength and vector direction are measured with a magnetometer from at least one location near the container to provide the container with a magnetic vector field tag and seal. The location(s) of the magnetometer relative to the container are also noted. If the position of any magnet inside the container changes, then the measured vector fields at the these locations also change, indicating that the tag has been removed, the seal has broken, and therefore that the container and objects inside may have been tampered with. A hollow wheel with magnets inside may also provide a similar magnetic vector field tag and seal. As the wheel turns, the magnets tumble randomly inside, removing the tag and breaking the seal.

  3. Concepts for backfilling and sealing of shafts

    International Nuclear Information System (INIS)

    Pierau, B.

    1990-01-01

    The disposal site is situated at a depth of 1000 to 1200 meters. It is covered by very thick cretatious mudstone layers forming the main barrier against the spread of radioactively contaminated water into the biosphere. Because of the excavation works and the resulting stress redistributions, the material surrounding the shafts is probably broken up, which leads to increased permeability in comparison with the intact rock. It is planned to backfill the shafts with an insoluble mineral mixture including a fine fraction necessary to achieve the sealing required. The joints and cracks in the brocken-up surrounding material are believed to be sealed by themselves due to swelling of the mudstone. Some strata of the mudstone contain more than 20% of smektite, a swelling clay mineral. Those regions, where the broken-up zone cannot be considered sure to self-seal due to swelling, are planned to be sealed by pressure grouting using clay suspension. (orig./HP) [de

  4. Valve seat pores sealed with thermosetting monomer

    Science.gov (United States)

    Olmore, A. B.

    1966-01-01

    Hard anodic coating provides a smooth wear resistant value seating surface on a cast aluminum alloy valve body. Vacuum impregnation with a thermosetting monomer, diallyl phthalate, seals the pores on the coating to prevent galvanic corrosion.

  5. HMSRP Hawaiian Monk Seal Survey Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains records of Hawaiian monk seal and green turtle sightings in the Northwestern Hawaiian Islands (NWHI) since 1982 at Lisianski Island, and since...

  6. Fuel cell cassette with compliant seal

    Science.gov (United States)

    Karl, Haltiner, Jr. J.; Anthony, Derose J.; Klotzbach, Darasack C.; Schneider, Jonathan R.

    2017-11-07

    A fuel cell cassette for forming a fuel cell stack along a fuel cell axis includes a cell retainer, a plate positioned axially to the cell retainer and defining a space axially with the cell retainer, and a fuel cell having an anode layer and a cathode layer separated by an electrolyte layer. The outer perimeter of the fuel cell is positioned in the space between the plate and the cell retainer, thereby retaining the fuel cell and defining a cavity between the cell retainer, the fuel cell, and the plate. The fuel cell cassette also includes a seal disposed within the cavity for sealing the edge of the fuel cell. The seal is compliant at operational temperatures of the fuel cell, thereby allowing lateral expansion and contraction of the fuel cell within the cavity while maintaining sealing at the edge of the fuel cell.

  7. HMSRP Hawaiian Monk Seal Tag Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains records for all tags applied to Hawaiian monk seals since 1981. These tags were applied by PSD personnel and cooperating scientists as part of...

  8. Management of spent sealed sources in Indonesia

    International Nuclear Information System (INIS)

    Wisnubroto, D.S.

    2002-01-01

    This paper describes the effort of the Center for Development of Radioactive Waste Management (CDRWM) to develop and implement activities in maintaining and improving the safety of spent sealed radiation sources and the security of radioactive materials over their life cycle. There is a wide variety of uses of radiation sources and radioactive materials in Indonesia, while the CDRWM plan to cover all spent radiation sources. Primary consideration is given to sealed radiation sources with relatively high levels of radioactivity which might necessitate interventional measures should control over them be lost. The policy of the Government of Indonesia for spent radiation sources is, whenever possible, spent sealed sources should be returned to the supplier. CDRWM has a general principle that sealed sources should not be removed from their holders, or the holders physically modified (except for Ra-226 needles, smoke detector and lighting preventer). (author)

  9. Inflatable Module Seal Interface Development and Testing

    Data.gov (United States)

    National Aeronautics and Space Administration — Develop a repeatable low permeable sealing interface evaluating O-ring, RTV bond and flowed RTV bond methods. Advanced Bladder materials (ArmorFlex, Nanoclay, etc)...

  10. HMSRP Hawaiian Monk Seal Photo Identification Database

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This photo collection contains identification and other images and video of Hawaiian monk seals taken by PSD personnel and cooperating scientists as part of the...

  11. Epoxy resins used to seal brachytherapy seed

    International Nuclear Information System (INIS)

    Ferreira, Natalia Carolina Camargos; Ferraz, Wilmar Barbosa; Reis, Sergio Carneiro dos; Santos, Ana Maria Matildes dos

    2013-01-01

    Prostate cancer treatment with brachytherapy is recommended for patients with cancer at an early stage. In this treatment, small radioactive seeds are implanted directly in the prostate gland. These seeds are composed at least of one radionuclide carrier and an X-ray marker enclosed within a metallic tube usually sealed by laser process. This process is expensive and, furthermore, it can provoke a partial volatilization of the radionuclide and change the isotropy in dose distribution around the seed. In this paper, we present a new sealing process using epoxy resin. Three kinds of resins were utilized and characterized by scanning electron microscopy (SEM), energy dispersive X ray (EDS) and by differential scanning calorimetry (DSC) after immersion in simulated body fluid (SBF) and in sodium iodine solution (NaI). The sealing process showed excellent potential to replace the sealing laser usually employed. (author)

  12. Crashworthy sealed pressure vessel for plutonium transport

    International Nuclear Information System (INIS)

    Andersen, J.A.

    1980-01-01

    A rugged transportation package for the air shipment of radioisotopic materials was recently developed. This package includes a tough, sealed, stainless steel inner containment vessel of 1460 cc capacity. This vessel, intended for a mass load of up to 2 Kg PuO 2 in various isotopic forms (not to exceed 25 watts thermal activity), has a positive closure design consisting of a recessed, shouldered lid fastened to the vessel body by twelve stainless-steel bolts; sealing is accomplished by a ductile copper gasket in conjunction with knife-edge sealing beads on both the body and lid. Follow-on applications of this seal in newer, smaller packages for international air shipments of plutonium safeguards samples, and in newer, more optimized packages for greater payload and improved efficiency and utility, are briefly presented

  13. HMSRP Hawaiian Monk Seal Necropsy Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains information on Hawaiian monk seal gross necropsy (in some cases only field notes or minimal information) and histopathology results beginning...

  14. Onboard Acoustic Recording from Diving Elephant Seals

    National Research Council Canada - National Science Library

    Fletcher, Stacia

    1996-01-01

    The aim of this project was to record sounds impinging on free-ranging northern elephant seals, Mirounga angustirostris, a first step in determining the importance of LFS to these animals as they dive...

  15. Risks of increased UV-B radiation: higher plants

    International Nuclear Information System (INIS)

    Rau, W.; Hofmann, H.

    1994-01-01

    The question pursued within the Bavarian climate research programme (BayFORKLIM) in the present context was as follows: Does the fact that UV-B radiation increases with growing site elevation mean that the low sensitivity of predominantly alpine plants compared with that of lowland plants is attributable to their different genetic constitution, possibly as a result of selective pressure and/or de alpine species have a greater capacity to develop protective mechanisms? Pairs and triplets of species belonging to the same genus but occuring at different site elevations were grown from seeds in a greenhouse that is, without UV-B. In order to determine their capacity to adapt to UV-B radiation, some of the plants were additionally exposed to UV-B for 5-6 weeks prior to sensitivity testing. Sensitivity was tested by exposing the plants to additional UV-B of different intensities in test chambers. Visible damage, ranging from light bronzing or yellowing to withering, served as an assessment criterion. Levels of UV-B absorbing substances (phenylpropane species, usually flavonoids) were also measured in these plants. The results obtained permit the following conclusions: The greater UV-B resistance of alpine species compared with that of lowland species of the same genus is not attributable to their genetic constitution but rather to their superior adaptability. Superior resistance is in part due to a greater accumulation of UV-B absorbing substances. Distinct differences in sensitivity between different genera could lead to population shifts within ecosystems as a result of increased UV-B radiation. (orig./KW) [de

  16. Sealing apparatus for airfoils of gas turbine engines

    Science.gov (United States)

    Jones, R.B.

    1998-05-19

    An improved airfoil tip sealing apparatus is disclosed wherein brush seals are attached to airfoil tips with the distal ends of the brush seal fibers sealingly contacting opposing wall surfaces. Embodiments for variable vanes, stators and both cooled and uncooled turbine blade applications are disclosed. 17 figs.

  17. 21 CFR 864.9750 - Heat-sealing device.

    Science.gov (United States)

    2010-04-01

    ... and Blood Products § 864.9750 Heat-sealing device. (a) Identification. A heat-sealing device is a device intended for medical purposes that uses heat to seal plastic bags containing blood or blood... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Heat-sealing device. 864.9750 Section 864.9750...

  18. The southern elephant seal Mirounga leonina at Gough Island

    African Journals Online (AJOL)

    haul-out in December coincided with the elephant seal moulting season, the elephant seals were found on the vegetated areas whilst the majority of fur seals hauled out onto the boulder beach. No interactions between the two species were recorded. Potential predators of elephant seals, i.e. the killer whales Orcinus orca ...

  19. Application of radioisotope technique for investigation of pumps seals

    International Nuclear Information System (INIS)

    Antoszewski, B.; Zorawska, A.

    1988-01-01

    Radioisotope method of measuring the wear of rings of the face mechanical seals, bush in soft seals, and leakage in double mechanical seals is described. The examples are given. It is found that radioisotope technique can be used for investigation of the seals. (author)

  20. Cover-gas-seal component development: dynamic inflatable-plug seal improvement

    International Nuclear Information System (INIS)

    Horton, P.H.

    1977-01-01

    This report documents the 1) radial compliance and 2) low friction coating tests conducted on the CRBRP Rotating Plug Inflatable Seals per test plan N707TR810014. Test results show that narrowing the seal blade from 0.25 to 0.12 in. will effectively reduce dynamic drag from 30 to 20 lb/ft under nominal conditions and will increase seal radial compliance from 0.12 to 0.30 in. without an unacceptable rise in dynamic drag. Tests also demonstrated that application of a teflon coating to the seal wear surface reduced breakaway drag by 25% based on results of comparison dwells

  1. Detection of seal contamination in heat-sealed food packaging based on active infrared thermography

    Science.gov (United States)

    D'huys, Karlien; Saeys, Wouter; De Ketelaere, Bart

    2015-05-01

    In the food industry packaging is often applied to protect the product from the environment, assuring quality and safety throughout shelf life if properly performed. Packaging quality depends on the material used and the closure (seal). The material is selected based on the specific needs of the food product to be wrapped. However, proper closure of the package is often harder to achieve. One problem possibly jeopardizing seal quality is the presence of food particles between the seal. Seal contamination can cause a decreased seal strength and thus an increased packaging failure risk. It can also trigger the formation of microchannels through which air and microorganisms can enter and spoil the enclosed food. Therefore, early detection and removal of seal-contaminated packages from the production chain is essential. In this work, a pulsed-type active thermography method using the heat of the sealing bars as an excitation source was studied for detecting seal contamination. The cooling profile of contaminated seals was recorded. The detection performance of four processing methods (based on a single frame, a fit of the cooling profile, pulsed phase thermography and a matched filter) was compared. High resolution digital images served as a reference to quantify contamination. The lowest detection limit (equivalent diameter of 0.63 mm) and the lowest processing time (0.42 s per sample) were obtained for the method based on a single frame. Presumably, practical limitations in the recording stage prevented the added value of active thermography to be fully reflected in this application.

  2. Anodizing And Sealing Aluminum In Nonchromated Solutions

    Science.gov (United States)

    Emmons, John R.; Kallenborn, Kelli J.

    1995-01-01

    Improved process for anodizing and sealing aluminum involves use of 5 volume percent sulfuric acid in water as anodizing solution, and 1.5 to 2.0 volume percent nickel acetate in water as sealing solution. Replaces process in which sulfuric acid used at concentrations of 10 to 20 percent. Improved process yields thinner coats offering resistance to corrosion, fatigue life, and alloy-to-alloy consistency equal to or superior to those of anodized coats produced with chromated solutions.

  3. Simulation of leakage through mechanical sealing device

    Science.gov (United States)

    Tikhomorov, V. P.; Gorlenko, O. A.; Izmerov, M. A.

    2018-03-01

    The procedure of mathematical modeling of leakage through the mechanical seal taking into account waviness and roughness is considered. The percolation process is represented as the sum of leakages through a gap between wavy surfaces and percolation through gaps formed by fractal roughness, i.e. the total leakage is determined by the slot model and filtration leakage. Dependences of leaks on the contact pressure of corrugated and rough surfaces of the mechanical seal elements are presented.

  4. UV induced thermoluminescence in rare earth oxide doped phosphors: possible use for UV dosimetry

    International Nuclear Information System (INIS)

    Yeh, S.-M.; Su, C.-S.

    1996-01-01

    UV induced thermoluminescent (TL) phenomena in some phosphors doped with rare earth oxides (Gd 2 O 3 :Eu, Gd 2 O 3 :Tb, Gd 2 O 3 :Dy and Y 2 O 3 :EU) have been investigated. Gd 2 O 3 :Eu and Y 2 O 3 :Eu have been found to possess prominent TL phenomena. A stable high temperature glow peak has been found at 345 o C in the cubic (C type) crystalline structure of Gd 2 O 3 :Eu. A more stable high temperature glow peak has also been found at about 380 o C in Y 2 O 3 :Eu. The sensitivity is high enough to be used as UV sensors. TL phenomena in Gd 2 O 3 :Tb and Gd 2 O 3 :Dy have also been investigated, but their TL intensities are much weaker than that of Gd 2 O 3 :Eu or Y 2 O 3 :Eu. On the other hand, all glow peaks of Gd 2 O 3 :Tb and Gd 2 O 3 :Dy are unstable at room temperature, therefore, Gd 2 O 3 :Tb and Gd 2 O 3 :Dy are not suitable for use as UV detectors. According to the above properties, the C type (cubic) crystalline structure of the Gd 2 O 3 :Eu phosphor seems to possess the potential of being the TL material for UV measurement. The position of the high temperature glow peak depends on the total UV exposure. It locates at about 380 o C when this phosphor was irradiated by 302 nm UV at 2.4 mJ.cm -2 exposure, but it shifts to 345 o C at 19.2 mJ.cm -2 or higher exposure. The response curves of this phosphor for various wavelengths, e.g. 253.7 nm, 302 nm, and 365 nm, were also measured. This phosphor is sensitive enough to measure background UV radiations, such as sunlight, bulb light etc. (author)

  5. Biobased Nanoparticles for Broadband UV Protection with Photostabilized UV Filters

    NARCIS (Netherlands)

    Hayden, D.R.|info:eu-repo/dai/nl/412640694; Imhof, A.|info:eu-repo/dai/nl/145641600; Velikov, K. P.|info:eu-repo/dai/nl/239483472

    2016-01-01

    Sunscreens rely on multiple compounds to provide effective and safe protection against UV radiation. UV filters in sunscreens, in particular, provide broadband UV protection but are heavily linked to adverse health effects due to the generation of carcinogenic skin-damaging reactive oxygen species

  6. 77 FR 20773 - Endangered and Threatened Species; Proposed Threatened Status for Subspecies of the Ringed Seal

    Science.gov (United States)

    2012-04-06

    ... sensitive or protected information. NMFS will accept anonymous comments (enter ``N/A'' in the required fields if you wish to remain anonymous). Attachments to electronic comments will be accepted in Microsoft...-2010-0258. By visiting the Internet at: http://alaskafisheries.noaa.gov/protectedresources/seals/ice...

  7. Analysis of ATLAS LTC-04R Test for Loop Seal Reformation Phenomena using RELAP5

    Energy Technology Data Exchange (ETDEWEB)

    Lim, Sang-Gyu; Kim, Dae-Hun; Kim, Han-Gon [KHNP CRI, Daejeon (Korea, Republic of)

    2016-10-15

    The loop seal reformation issue was selected to be the analysis topic of the DSP-04 based on the technical discussion between the participants and the operating agencies (KAERI and KINS) and domestic experts meetings. After that, KAERI performed LTC-04R test which is 4 inch top-slot cold-leg break test using ATLAS facility in December 27, 2015. KHNP CRI, as a participant of the DSP-04, performed the blind calculation and open calculation using RELAP5/Mod3.3 patch 3. This paper deals with the results of open calculation for ATLAS LTC-04R test. The results of several sensitivity analyses such as the critical flow modeling sensitivity and break flow system modeling sensitivity will be discussed. Several possible factors in the loop seal reformation behavior are examined in the sensitivity analysis. Heat loss modeling, fine break system modeling, fine loop seal nodalization and off-take modeling are not significant factor in the loop seal reformation. Still critical flow model and discharge coefficient are dominant factors. Based on the ATLAS LTC-04R, Ransom-Trapp model shows better prediction in the break flow than the Henry-Fauske model.

  8. Tamper-indicating devices and safeguards seals evaluation test report

    International Nuclear Information System (INIS)

    Horton, P.R.V.; Waddoups, I.G.

    1993-12-01

    Sandia National Laboratories was asked to evaluate the seals used as tamper-indicating devices (TIDs) at DOE facilities. Initially, a survey determined what seal manufacturers were being used and what similar seal types were available. Once the required specifications for TIDs were defined, a test plan measured the currently available seals against the requirements. Environmental and physical type tests stressed the seals under two broad categories: (1) handling durability and (2) tamper resistance. Results of the testing provide comparative ratings for the various seals, recommendations for using currently available seals, and a new tamper-indicating technology

  9. Metal and elastomer seal tests for accelerator applications

    International Nuclear Information System (INIS)

    Welch, K.M.; McIntyre, G.T.; Tuozzolo, J.E.; Skelton, R.; Pate, D.J.; Gill, S.M.

    1989-01-01

    The vacuum system of the Alternating Gradient Synchrotron (AGS) at Brookhaven National Laboratory has more than a thousand metal vacuum seals. Also, numerous elastomer seals are used throughout the AGS to seal large beam component chambers. An accelerator upgrade program is being implemented to reduce the AGS operating pressure by x100 and improve the reliability of the vacuum system. This paper describes work in progress on metal and elastomer vacuum seals to help meet those two objectives. Tests are reported on the sealing properties of a variety of metal seals used on different sealing surfaces. Results are also given on reversible sorption properties of certain elastomers. 16 refs., 6 figs., 4 tabs

  10. Reactor coolant pump shaft seal stability during station blackout

    International Nuclear Information System (INIS)

    Rhodes, D.B.; Hill, R.C.; Wensel, R.G.

    1987-05-01

    Results are presented from an investigation into the behavior of Reactor Coolant Pump shaft seals during a potential station blackout (loss of all ac power) at a nuclear power plant. The investigation assumes loss of cooling to the seals and focuses on the effect of high temperature on polymer seals located in the shaft seal assemblies, and the identification of parameters having the most influence on overall hydraulic seal performance. Predicted seal failure thresholds are presented for a range of station blackout conditions and shaft seal geometries

  11. Reactor coolant pump shaft seal stability during station blackout

    Energy Technology Data Exchange (ETDEWEB)

    Rhodes, D B; Hill, R C; Wensel, R G

    1987-05-01

    Results are presented from an investigation into the behavior of Reactor Coolant Pump shaft seals during a potential station blackout (loss of all ac power) at a nuclear power plant. The investigation assumes loss of cooling to the seals and focuses on the effect of high temperature on polymer seals located in the shaft seal assemblies, and the identification of parameters having the most influence on overall hydraulic seal performance. Predicted seal failure thresholds are presented for a range of station blackout conditions and shaft seal geometries.

  12. HFCVD Diamond-Coated Mechanical Seals

    Directory of Open Access Journals (Sweden)

    Raul Simões

    2018-05-01

    Full Text Available A mechanical seal promotes the connection between systems or mechanisms, preventing the escape of fluids to the exterior. Nonetheless, due to extreme working conditions, premature failure can occur. Diamond, due to its excellent properties, is heralded as an excellent choice to cover the surface of these devices and extend their lifetime. Therefore, the main objective of this work was to deposit diamond films over mechanical seals and test the coated seals on a water pump, under real working conditions. The coatings were created by hot filament chemical vapor deposition (HFCVD and two consecutive layers of micro- and nanocrystalline diamond were deposited. One of the main difficulties is the attainment of a good adhesion between the diamond films and the mechanical seal material (WC-Co. Nucleation, deposition conditions, and pre-treatments were studied to enhance the coating. Superficial wear or delamination of the film was investigated using SEM and Raman characterization techniques, in order to draw conclusions about the feasibility of these coatings in the WC-Co mechanical seals with the purpose of increasing their performance and life time. The results obtained gave a good indication about the feasibility of this process and the deposition conditions used, with the mechanical seals showing no wear and no film delamination after a real work environment test.

  13. Resistance of human and mouse myeloid leukemia cells to UV radiation

    International Nuclear Information System (INIS)

    Poljak-Blazi, M.; Osmak, M.; Hadzija, M.

    1989-01-01

    Sensitivity of mouse bone marrow and myeloid leukemia cells and sensitivity of human myeloid leukemia cells to UV light was tested. Criteria were the in vivo colony-forming ability of UV exposed cells and the inhibition of DNA synthesis during post-irradiation incubation for 24 h in vitro. Mouse bone marrow cells irradiated with a small dose of UV light (5 J/m 2 ) and injected into x-irradiated animals did not form hemopoietic colonies on recipient's spleens, and recipients died. However, mouse leukemia cells, after irradiation with higher doses of UV light, retained the ability to form colonies on the spleens, and all recipient mice died with typical symptoms of leukemia. In vitro, mouse bone marrow cells exhibited high sensitivity to UV light compared to mouse myeloid leukemia cells. Human leukemia cells were also resistant to UV light, but more sensitive than mouse leukemia cells. (author)

  14. Design and research of seal structure for thermocouple column assembly

    International Nuclear Information System (INIS)

    Rao Qiqi; Li Na; Zhao Wei; Ma Zhigang

    2015-01-01

    The new seal structure was designed to satisfy the function of thermocouple column assembly and the reactor structure. This seal structure uses the packing graphite ring and adopts the self-sealing principle. Cone angle is brought to the seal face of seal structure which is conveniently to assembly and disassembly. After the sealing principle analysis and stress calculation of graphite ring which adopt the cone angle, the cone angle increases the radial force of seal structure and improves the seal effect. The stress analysis result shows the seal structure strength satisfies the regulation requirement. The cold and hot function test results shows the sealing effect is good, and the design requirement is satisfied. (authors)

  15. Application study of magnetic fluid seal in hydraulic turbine

    International Nuclear Information System (INIS)

    Yu, Z Y; Zhang, W

    2012-01-01

    The waterpower resources of our country are abundant, and the hydroelectric power is developed, but at present the main shaft sealing device of hydraulic turbine is easy to wear and tear and the leakage is great. The magnetic fluid seal has the advantages of no contact, no wear, self-healing, long life and so on. In this paper, the magnetic fluid seal would be used in the main shaft of hydraulic turbine, the sealing structure was built the model, meshed the geometry, applied loads and solved by using MULTIPHYSICS in ANSYS software, the influence of the various sealing structural parameters such as tooth width, height, slot width, sealing gap on the sealing property were analyzed, the magnetic fluid sealing device suitable for large-diameter shaft and sealing water was designed, the sealing problem of the hydraulic turbine main shaft was solved effectively which will bring huge economic benefits.

  16. A dynamic analysis of rotary combustion engine seals

    Science.gov (United States)

    Knoll, J.; Vilmann, C. R.; Schock, H. J.; Stumpf, R. P.

    1984-01-01

    Real time work cell pressures are incorporated into a dynamic analysis of the gas sealing grid in Rotary Combustion Engines. The analysis which utilizes only first principal concepts accounts for apex seal separation from the crochoidal bore, apex seal shifting between the sides of its restraining channel, and apex seal rotation within the restraining channel. The results predict that apex seals do separate from the trochoidal bore and shift between the sides of their channels. The results also show that these two motions are regularly initiated by a seal rotation. The predicted motion of the apex seals compares favorably with experimental results. Frictional losses associated with the sealing grid are also calculated and compare well with measurements obtained in a similar engine. A comparison of frictional losses when using steel and carbon apex seals has also been made as well as friction losses for single and dual side sealing.

  17. Mechanical seal having a double-tier mating ring

    Science.gov (United States)

    Khonsari, Michael M.; Somanchi, Anoop K.

    2005-09-13

    An apparatus and method to enhance the overall performance of mechanical seals in one of the following ways: by reducing seal face wear, by reducing the contact surface temperature, or by increasing the life span of mechanical seals. The apparatus is a mechanical seal (e.g., single mechanical seals, double mechanical seals, tandem mechanical seals, bellows, pusher mechanical seals, and all types of rotating and reciprocating machines) comprising a rotating ring and a double-tier mating ring. In a preferred embodiment, the double-tier mating ring comprises a first and a second stationary ring that together form an agitation-inducing, guided flow channel to allow for the removal of heat generated at the seal face of the mating ring by channeling a coolant entering the mating ring to a position adjacent to and in close proximity with the interior surface area of the seal face of the mating ring.

  18. Performances of Magnetic Fluid Seal and Application to Turbopumps

    OpenAIRE

    北洞, 貴也; 黒川, 淳一; 宮副, 雄貴; 林, 正悦

    1994-01-01

    A magnetic fluid shaft seal can achieve zero-leakage and operate stably against shaft vibration, but the sealing pressure is very low. In order to improve the pressure performance of a magnetic fluid seal and apply it to a turbopump, the seal pressure characteristics are studied theoretically and experimentally. The Poisson equation for magnetic vector potential is solved by FEM, and the seal performances are determined by use of the Bernoulli equation. The validity of the theory is confirmed...

  19. Seroprevalence of Antibodies against Seal Influenza A(H10N7) Virus in Harbor Seals and Gray Seals from the Netherlands.

    Science.gov (United States)

    Bodewes, Rogier; Rubio García, Ana; Brasseur, Sophie M; Sanchez Conteras, Guillermo J; van de Bildt, Marco W G; Koopmans, Marion P G; Osterhaus, Albert D M E; Kuiken, Thijs

    2015-01-01

    In the spring and summer 2014, an outbreak of seal influenza A(H10N7) virus infection occurred among harbor seals (Phoca vitulina) off the coasts of Sweden and Denmark. This virus subsequently spread to harbor seals off the coasts of Germany and the Netherlands. While thousands of seals were reported dead in Sweden, Denmark and Germany, only a limited number of seals were found dead in the Netherlands. To determine the extent of exposure of seals in the Netherlands to influenza A/H10N7 virus, we measured specific antibody titers in serum samples from live-captured seals and seals admitted for rehabilitation in the Netherlands by use of a hemagglutination inhibition assay and an ELISA. In harbor seals in 2015, antibodies against seal influenza A(H10N7) virus were detected in 41% (32 out of 78) pups, 10% (5 out of 52) weaners, and 58% (7 out of 12) subadults or adults. In gray seals (Halichoerus grypus) in 2015, specific antibodies were not found in the pups (n = 26), but in 26% (5 out of 19) of the older animals. These findings indicate that, despite apparent low mortality, infection with seal influenza A(H10N7) virus was geographically widespread and also occurred in grey seals.

  20. Standardization of UV LED measurements

    Science.gov (United States)

    Eppeldauer, G. P.; Larason, T. C.; Yoon, H. W.

    2015-09-01

    Traditionally used source spectral-distribution or detector spectral-response based standards cannot be applied for accurate UV LED measurements. Since the CIE standardized rectangular-shape spectral response function for UV measurements cannot be realized with small spectral mismatch when using filtered detectors, the UV measurement errors can be several times ten percent or larger. The UV LEDs produce broadband radiation and both their peaks or spectral bandwidths can change significantly. The detectors used for the measurement of these LEDs also have different spectral bandwidths. In the discussed example, where LEDs with 365 nm peak are applied for fluorescent crack-recognition using liquid penetrant (non-destructive) inspection, the broadband radiometric LED (signal) measurement procedure is standardized. A UV LED irradiance-source was calibrated against an FEL lamp standard to determine its spectral irradiance. The spectral irradiance responsivity of a reference UV meter was also calibrated. The output signal of the reference UV meter was calculated from the spectral irradiance of the UV source and the spectral irradiance responsivity of the reference UV meter. From the output signal, both the integrated irradiance (in the reference plane of the reference meter) and the integrated responsivity of the reference meter were determined. Test UV meters calibrated for integrated responsivity against the reference UV meter, can be used to determine the integrated irradiance from a field UV source. The obtained 5 % (k=2) measurement uncertainty can be decreased when meters with spectral response close to a constant value are selected.